LOCUS       DATGFG010000001         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_685131, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000001 DATGFG010000000
VERSION     DATGFG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685131"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VKQ52_00005"
     CDS             complement(<1..241)
                     /locus_tag="VKQ52_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HLZ85578.1"
                     /translation="MAIVTITPAGLSREEVILLTGKYGKNEFSADKQGGFLRKLWDIA
                     REPMFVILIIACLLYFVLGEIKEGLLMAAAMVFVGA"
     gene            401..1492
                     /locus_tag="VKQ52_00010"
     CDS             401..1492
                     /locus_tag="VKQ52_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLZ85579.1"
                     /translation="MAENNSSAGNPGAIAKYLQIVLFTVVLLYLGRSLFIPLFFGLLA
                     ALLLYPVSHWLERRHWPRSLAISLLLSLLVLVFLALIGLLGMEMNTFIRDIPKISHRL
                     AQLADTIQNWIERTYGMARDTQAGWIDKMMADAGNSVSATLRSLLNSTASTVIMLVMV
                     PVYAALFLYHRGTFVQFVERLAGEELRPRIQLILRQSILSYFNYVKGTFFVYCIVGAL
                     NTIGLFALGIENALLYGMLTSFMMVIPYIGIFISASIPVSIALVTKDSAWYPVAVVAV
                     FAFIQYLESNVIFPRVVGAQLNLSTWATLVAIVAGTLLWGLSGMILFTPFLAILKIVS
                     DNIPEWQPLNILLSRSYGYKGKTAQPLLK"
     gene            1522..2100
                     /locus_tag="VKQ52_00015"
     CDS             1522..2100
                     /locus_tag="VKQ52_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLZ85580.1"
                     /translation="MNRRAYWMVNGITLYRIVAALAMVVVIFWGRWELFKWLLPISFL
                     TDAVDGYLARRYKVVSAVGATLDSIGDDLTVLVAFAGLIIYAPGFFKKEIIPVAVLGA
                     LYLTQTVMALIRYRKITSFHTYLAKMAAISQGVFLILTFFLPAVPLILFWMAAILTAL
                     DLVEETIMVLLLPVWRPDVKGLSAIWRKKPPE"
     gene            2158..>2717
                     /locus_tag="VKQ52_00020"
     CDS             2158..>2717
                     /locus_tag="VKQ52_00020"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HLZ85581.1"
                     /translation="MHTNTLSLRSGPKPAVFFMQQKKFILTAFLIGLSTLLTAGGPAQ
                     LVFSPTTIPDGLYGSAYKTQDLKVTGGKAPYSFSISGGSLPPGMSLSGAGVISGTPIA
                     AGAYSFAVTALDNTPGPGRLSGSQNYTLKVGQASLTITANDATATYGGAVPALTASYS
                     GFVNGDNISHLTTRPALSTTATSSSPA"
BASE COUNT          571 a          832 c          636 g          678 t
ORIGIN      
        1 tcgcgcccac aaataccatg gctgcggcca tcagcagtcc ctccttgatc tcgcccaaca
       61 caaaatagag caggcaggca atgatcaata ttacaaacat gggttccctg gcgatatccc
      121 acagcttccg gagaaatcct ccctgcttat cagccgaaaa ctcattctta ccatactttc
      181 cagtgaggag gattacctct tcccggctta atcccgcagg tgtaatagta acgatagcca
      241 tcgtgcgaag gttttacata tatcctacga gcgacatgat ggagatcatt gcaaaatatg
      301 atttgacgcc ttttctgaca agaatcatgt ggtccacgga tccttgtcat tccgggggcc
      361 cttcagccgg cctactttga ccccctataa cacgatctat atggccgaga acaattccag
      421 cgccgggaat ccgggcgcca ttgctaaata cctccagata gtcctgttca ccgtcgtgct
      481 tctttacctg ggaagatcgc tttttattcc tttgttcttc ggccttttgg ccgccctcct
      541 gctttatccc gtctctcact ggctggaaag aaggcattgg cccagaagcc ttgccatcag
      601 tttactgctt tccctgttgg tgctggtatt cctcgccctt ataggtctgc tgggaatgga
      661 aatgaacact tttatccggg acatacccaa aatatcccat cggctggccc agttggccga
      721 taccatccaa aactggatcg aacgtaccta cggtatggcc cgcgacaccc aggccggctg
      781 gatcgacaag atgatggccg atgccgggaa tagtgtaagc gccaccctca ggagcctgtt
      841 gaattccact gcctccaccg tcattatgct cgtaatggta cccgtctatg ccgccctctt
      901 tctataccac cggggcacat tcgttcagtt cgtggaacgg cttgccggtg aagaactgcg
      961 gccacgcata caactcatcc tgcgccagag cattctcagc tatttcaact atgtcaaagg
     1021 aaccttcttt gtctattgca tcgtcggcgc tctcaatacc atcggcctgt ttgcgctggg
     1081 tatcgaaaat gcgctgttat atggcatgct cacctccttt atgatggtca tcccctacat
     1141 cggcattttt atcagcgcct ccattcccgt ctccattgcg ctggtgacca aggactccgc
     1201 ctggtatcct gttgccgtcg tcgccgtttt tgcctttatc caataccttg aatcaaacgt
     1261 catctttccc agggtcgtcg gcgcccagct aaacctgagc acatgggcca ccctggtcgc
     1321 catcgtggcg gggaccctcc tgtggggtct gtccggcatg atcctgttca ccccctttct
     1381 tgccatcctg aagatcgtct ccgacaacat tcccgaatgg cagccattga atatcttatt
     1441 aagccgatcg tatgggtaca aggggaaaac cgctcaacca cttttaaaat gaatggcagt
     1501 agctttgcac caaatcatcg catgaaccgg cgcgcatact ggatggtcaa cggcattacc
     1561 ctttatcgta tcgtggcggc tctcgccatg gtagtggtca ttttttgggg ccgttgggag
     1621 ctattcaaat ggctgcttcc cataagcttt ctaactgacg ccgtggatgg ctacctcgcc
     1681 agaagataca aagtcgtcag tgcggtgggc gccaccctcg actcgatagg cgacgatctc
     1741 acggtcctgg tcgcctttgc cggccttatt atttatgcgc ccggcttctt caagaaggaa
     1801 attatccccg tggccgtact tggcgcatta tacctcaccc aaaccgtcat ggccctgatc
     1861 cgttaccgga aaataacgag cttccatacg tatcttgcaa agatggccgc catctcccag
     1921 ggcgtcttcc tgatcctcac cttctttttg cccgctgttc cccttattct gttctggatg
     1981 gccgccatcc ttacggcgct cgacctcgtg gaagagacga taatggtcct cctgctaccc
     2041 gtctggcggc ccgacgtaaa gggcctgtcg gccatttgga ggaagaaacc gccggagtaa
     2101 cggcaggccg aaaataaagg gaatgagaat tgcagtaggt taggttacta agcctatatg
     2161 catacaaaca ccctttccct acgcagtggt ccaaagccgg ctgtcttctt tatgcaacag
     2221 aagaagttca tccttacggc ctttttgata ggattgagca ctctgctgac tgccggcgga
     2281 cccgcgcaac tggtcttttc cccaacaacg attcccgatg ggctgtatgg ctcagcctat
     2341 aaaacccagg acctgaaggt caccggcggt aaagccccct attccttctc tatttcgggc
     2401 ggcagcctgc ctccgggcat gtccttatcc ggcgcagggg ttatttccgg cacacctatc
     2461 gctgccgggg cctattcatt tgccgttacc gccctggaca acaccccggg acccggccgg
     2521 ctttcgggct cccaaaacta taccctgaaa gtcgggcagg cctccctgac tattaccgcc
     2581 aacgatgcca ccgccaccta tggaggcgcg gttccggcgc tgacagcgtc ctacagcggc
     2641 tttgtcaacg gggacaatat ctcccatctg accacccggc cggcccttag cacgacggcc
     2701 acctcctcct cgccggc
//
LOCUS       DATGFG010000002         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_684990, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000002 DATGFG010000000
VERSION     DATGFG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684990"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..488)
                     /locus_tag="VKQ52_00025"
     CDS             complement(159..488)
                     /locus_tag="VKQ52_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85582.1"
                     /translation="MKKAILFSCILLAVASTTTKTYAQPPRIAIEVIIGSRPPTPNEA
                     QLMRAEESKHPNITRAMHDIDASIRALHDAPDDFGGHKGQAESDLHAAYISLRKALYF
                     RLYQDTH"
     gene            770..952
                     /locus_tag="VKQ52_00030"
     CDS             770..952
                     /locus_tag="VKQ52_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85583.1"
                     /translation="MNTREYIKMEGKEEGKEEATTAFVENLLKGSDFAVEKIAFLANV
                     SVEFVNKIKSQLNGTR"
     gene            1219..2394
                     /locus_tag="VKQ52_00035"
     CDS             1219..2394
                     /locus_tag="VKQ52_00035"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ85584.1"
                     /translation="MIKRRLEASIRDALKRSPSVALMGPRQVGKTTIALNISETMPSV
                     YLDLENRLDLEKVRDIASFHADNRDKLIILDEVQRLPEVFAPLRGIIDKERRKGNKFG
                     QFLFLGSASIDLLQQSSESLAGRIAYIELFPVDVLEYAADSIDKMHQLWLRGGFPESL
                     LAASESDSLDWRRDFIKTYLERDIPQLGPRIPAETLERFWTMLAHNQGAVLNASQLGR
                     SLEVSSVTVGRYLDLMADLLLVRRLKPWTFNIGKRLVRSPKVYIRDSGITHALLNIDT
                     YNNLLGHPVVGGSWEGFVIENIMSVAPSRVQPFYYGTPGGAEIDLILELSPTEKWAIE
                     IKRSSSPSVSKGFHVACDDIKPQQRYVVYSGKDRFSLGERITAISLSELMQEILSLS"
     gene            2529..>3304
                     /locus_tag="VKQ52_00040"
     CDS             2529..>3304
                     /locus_tag="VKQ52_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HLZ85585.1"
                     /translation="MKRIFTLLVLLLALTRAFPQLPPGAPGGSAGNGGRPGGGPASGH
                     IYGKLVDSAGKGIARASVLILKVMIDAGGKRKEMLVKGLTTQGNGDFSAEDLPVNTPL
                     KLKASAVGYTEWSQEITLHPPTTDKDLGNLALTAKASELQQVVVTASKPAMSVDMDKK
                     VFNVAKDIVSAGGTGLDVLKNVPSINVDIDGNVTLRGGSPQIMVDGKPTTLTLDEIPS
                     DAIESVEVITNPSAKYDASGGGAGILNIVLKKNRKSGYNG"
BASE COUNT          824 a          894 c          828 g          758 t
ORIGIN      
        1 ccgaaggaag gctaaaggag gcctttttat atcagttccg ggtgtcactc gaacattata
       61 cccagctgct cccaacccgg ggatagcgca acaaaaaagc cgtcccaccc gggacggcca
      121 ttctaccaaa tttcgtcagc cggatcaccg gcgttttgtt aatgcgtatc ctggtaaagc
      181 cggaaataaa gggctttgcg cagggagata taggctgcat gcaggtcgga ttcggcctgg
      241 cctttgtgac caccgaaatc atcaggggcg tcatgcaggg cccgtataga tgcgtcgatg
      301 tcgtgcatgg cccgcgtaat gttgggatgc ttggactctt cagcccgcat caactgggct
      361 tcgttcggag tgggcggccg gctcccgatg atcacctcga ttgcgattct gggcggctgt
      421 gcgtaggttt tggttgtagt agaagcaacc gccagcaaaa tgcacgagaa aaggatagct
      481 tttttcatag ctttttttaa gattggttaa aaaatgaaaa ttggatgttc gacagtcttt
      541 acccgggaga gtttaatccc cgggaaggca tttttcagaa aggagtacct gtaaacgagg
      601 catatgaatc atttatttga tgcaacccgc aaaattttcg caacccggta tcaggaacgg
      661 atgggttccc cggcgggcga atgaccgttc caaacttttt gaggaattac ctccggaaac
      721 aaatcgtaaa tttgataagg aaattaaaga aacagataaa gccggtgtta tgaatacacg
      781 tgaatatata aaaatggaag ggaaggaaga aggaaaggaa gaggcaacta cggcttttgt
      841 cgaaaaccta ttgaaggggt ccgactttgc tgtggagaag atcgccttcc tggctaatgt
      901 ctctgtggag ttcgtaaaca agatcaagag ccaattaaac gggaccaggt aggctacctg
      961 tcccttatct tatacgaccg ccggtaaccc cggcggtttt ttatttgatg cctgaaatct
     1021 tattgatcag aaacgccaga atagcccatc gatgcaaggc ccaaacaagc gcgatgaaaa
     1081 ataatgcagt gatgatgcaa aaaatgatgc ggaacccaag ggctaaaact ataaaaaatc
     1141 ataaataatt gatagtgact gatttgtgtc tattcaattt actctgcaaa ttttacattt
     1201 tgtgttaaat ttgcagagat gattaaaaga cgactcgaag catcaatccg tgacgcttta
     1261 aagcgtagtc cgtccgtggc cctgatggga ccgcgtcagg tgggcaaaac caccattgcc
     1321 ctgaatatat ccgaaaccat gccttctgtc tatttggacc ttgaaaatcg tcttgatctg
     1381 gaaaaggtcc gggacattgc ctccttccac gctgacaatc gcgacaagct gatcatcctc
     1441 gacgaagtcc agcgactacc ggaagtcttt gcgccacttc gcggtatcat cgataaagag
     1501 cgccgcaaag gaaataaatt cggacagttt ctgttccttg gatctgcttc cattgacttg
     1561 ctacaacaat ccagcgaatc ccttgccggc cgcattgcct acatcgaatt atttcccgtt
     1621 gacgtgctgg aatatgccgc ggacagcatc gataaaatgc accagttatg gttgcgaggc
     1681 ggttttcccg aaagcctttt ggcggcatct gaatccgaca gccttgactg gcgccgggat
     1741 tttatcaaaa cctatttaga gcgggatatt ccccagcttg gtcctcgcat tcccgccgaa
     1801 acccttgaac gtttctggac catgctggcg cataaccagg gggctgtact gaatgcgtcc
     1861 caattgggac gaagccttga agtttccagc gtgacggtgg gccgctatct tgatctaatg
     1921 gccgatctct tattggtcag acgcctcaag ccctggacgt tcaatatcgg caagcgtctg
     1981 gtccgttctc caaaagtcta tatccgggac agcggcatta ctcacgccct actgaatatt
     2041 gatacctaca ataatctttt ggggcaccct gtcgtcggtg gcagttggga aggttttgtc
     2101 attgaaaata ttatgtcagt ggctccttcc agggtgcaac ctttttacta cggcacacct
     2161 ggaggggcgg agatcgacct gatcctggaa ctttctccaa cggaaaaatg ggcgatcgag
     2221 atcaagcgca gctcgtcgcc gtccgtttcc aaagggtttc atgtggcctg cgacgatatc
     2281 aaaccacaac aacgttatgt ggtttattcc ggcaaggata ggttctcgct aggcgagcgg
     2341 atcacagcga tttcactatc cgaactaatg caggaaattc ttagtctgtc ttgatcagac
     2401 caattggcgc cgcaggcctt cctatccccc catccccact ccgcacactt ttcccggttg
     2461 accgcagact tttccgggtt actcgcaagc atccccaacg gtccctctcc actatctagt
     2521 tttgaggcat gaaacgcata tttaccctcc tggtcctcct cctcgcgcta acccgcgcct
     2581 tcccgcaact ccctcccggc gcccccggtg gctccgccgg caatggcggc cgccctggcg
     2641 gaggccccgc ctcgggccat atatatggaa aactggtcga ctcggccggc aagggtatcg
     2701 cccgtgcttc cgtgctgatc ctcaaagtga tgatcgacgc cggcggcaaa cgcaaagaaa
     2761 tgctggtaaa aggcctcacc acgcagggca acggcgactt ctccgccgaa gacctgcccg
     2821 tcaatacccc gctgaagctg aaagcatccg cggtcggcta caccgaatgg agccaggaga
     2881 tcaccctgca tccccccacc acggataaag acctcggcaa cctcgctctc acggcgaagg
     2941 ccagcgaact ccagcaggtg gtcgtcacgg cctccaaacc cgccatgtcc gtcgatatgg
     3001 ataaaaaggt cttcaacgtc gccaaggata tcgtcagcgc cggcggcacc ggtctcgatg
     3061 tcctcaagaa tgtccccagt atcaacgtgg acatcgacgg caacgtcacc cttcgcggcg
     3121 gctcgcccca gatcatggtg gacggcaagc ccaccaccct cacactggac gagatcccct
     3181 ccgatgccat cgaaagcgtg gaggtgatca ccaacccctc ggcaaaatac gatgcctccg
     3241 gcggcggcgc gggcatcctg aatatcgtcc tgaagaagaa ccgtaagtcc ggctataacg
     3301 gcag
//
LOCUS       DATGFG010000003         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1255700, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000003 DATGFG010000000
VERSION     DATGFG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255700"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VKQ52_00045"
     CDS             <1..1494
                     /locus_tag="VKQ52_00045"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ85586.1"
                     /translation="FSNGDGWLGNSYFSLPYGYAELLGDMVGNEGASNQLISQVNVPN
                     YYILDDGSKVTNSSWNKSTLRLNNTRAQTGAGYNPTYYQWLNMYALNNACNQVLVLIP
                     TIKFSGDTTTKANTLKAWCYWWKGYAYASIGSMYYSGLLIDTYGKTTDHYVVKDSILA
                     RSDYYYNQAATLLGSITNTSDYSTILGQLIPAFCQVGNGGVLTPAMWIRNINTMLARN
                     LLLNKLAPFVNGNPSAMISGGSMTAMSSSDWTNVLNLATNGIKQGDYVFTGRASSASG
                     FFTAAGGTVSALATGPNGSTTFKVGERFLQYFKTGDLRMSTNFNSNTKYNADFFTTRY
                     SMISGGTGTSGVYVYGDKTPGAYELFIAGSYEENALMLAEANIRLGNISQGLSYVDAI
                     RTYQGAGISAVSGTVTTLAGALQELVAERRVALAFRGLSYYDIRRWGWTYDISQGGGS
                     YHNTVFGTTLNTNVTINYNFLDYWDVPADESVLNPPGSGSAPVLNPN"
     gene            1520..>2001
                     /locus_tag="VKQ52_00050"
     CDS             1520..>2001
                     /locus_tag="VKQ52_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85587.1"
                     /translation="MTKIILLLIISTAGIRTLGQSGSAEGSRFDLMELFDATGKRFVN
                     PPSDIAGSPYLQQEWLKAILITDKGKRFENIRIRLDLEKQEIHFTAREGYEAVAPQGL
                     IKAFTLLDSSLDYPPGAEYQCGFPSVDGKTVFNFYQVLAGGKFMMLRAPYKTIVKHKG
                     "
BASE COUNT          507 a          566 c          480 g          448 t
ORIGIN      
        1 ttcagcaatg gcgacggctg gctcggaaac agctatttct cccttcccta cggttacgcc
       61 gaacttctcg gtgacatggt aggaaatgaa ggcgcttcaa accaattgat aagccaggtc
      121 aacgtgccca attactatat cctcgatgac ggttcgaaag tgaccaatag ttcctggaat
      181 aaatccacac tgcggctcaa caatacaagg gcccagacag gtgccggcta caaccccacc
      241 tattaccagt ggttgaatat gtacgcgctt aataatgcct gcaaccaggt attggtgttg
      301 atccctacca tcaaattctc gggagacaca acaacaaaag ccaataccct caaggcctgg
      361 tgctattggt ggaaagggta tgcctatgcc tccatcggct ccatgtacta ttccggtctg
      421 ctcatagata cttacggcaa gaccaccgat cactacgtcg ttaaagattc gatccttgcc
      481 cgatcggact actattacaa tcaggccgcc acactcctgg gctcgatcac caatacgtcg
      541 gattatagca cgatactggg acaactcata cctgctttct gccaggtagg caacggtggc
      601 gtcctgacac ccgccatgtg gatccgcaat atcaacacca tgctggcgcg caacctcctg
      661 ctgaacaaat tggccccctt tgtcaacggc aacccctccg ccatgatcag tggcggctct
      721 atgaccgcga tgagctcttc ggattggacc aatgtactca acctggcaac caatggtata
      781 aaacaaggag attatgtgtt taccggcagg gccagctcgg cttccggatt ctttaccgcg
      841 gcaggcggta ccgtgtccgc cctggccaca ggccccaatg gctccaccac cttcaaggtg
      901 ggagaacggt tcctccagta cttcaagaca ggagatcttc gcatgtcgac caatttcaat
      961 agcaacacga aatataatgc ggatttcttc accaccaggt acagtatgat cagcggtggc
     1021 accggtacgt caggggtcta tgtttatgga gataaaaccc cgggtgcata tgagctcttt
     1081 atagccggta gctacgaaga gaatgcgctg atgctggcag aggctaatat ccggctggga
     1141 aatatcagcc agggcttatc ctatgtagac gccatacgca cctatcaggg tgccggcatc
     1201 tccgccgttt ccggaacggt aaccacactt gccggcgcgc tgcaggaact ggttgccgaa
     1261 cgccgtgtcg cacttgcatt cagaggcctc tcttactacg atatcagacg ctggggctgg
     1321 acctatgata tctcccaggg aggcggtagc tatcacaaca ccgtttttgg aactacgctc
     1381 aacaccaacg ttaccatcaa ttacaacttc ctggactatt gggatgttcc ggccgacgaa
     1441 tcggttctta acccgccggg ctcgggaagc gcaccggtcc tgaaccctaa ttaaacacaa
     1501 accggatcgc tgattttcta tgacaaagat catccttctg ttgatcattt ccaccgcagg
     1561 catccgcacg ctgggccagt ccggctccgc ggaagggtcg cgctttgatc tgatggaact
     1621 gttcgacgcc acaggaaagc gattcgtcaa cccgccttcc gatattgccg ggtctcccta
     1681 ccttcagcag gaatggctca aggccatcct gatcaccgac aaagggaaaa gattcgaaaa
     1741 tatcaggata aggctggacc tcgaaaaaca agagatccat ttcaccgcca gggaaggata
     1801 cgaagccgtg gcgccgcagg gattgatcaa agcatttacc ctgctcgact cctccctgga
     1861 ctatcctccc ggcgccgaat atcaatgcgg gtttccatcc gtcgatggta aaacggtctt
     1921 taatttctac caggtattgg cgggaggaaa atttatgatg ctccgggctc cttacaaaac
     1981 catagtaaaa cataaaggtg a
//
LOCUS       DATGFG010000004         4475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_684334, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000004 DATGFG010000000
VERSION     DATGFG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684334"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VKQ52_00055"
     CDS             complement(<1..619)
                     /locus_tag="VKQ52_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ85588.1"
                     /translation="MPQKPVLSVVIPVYNEAENLPALLRDWQPVFRSTGAPYRVIVID
                     DGSRDDSLRLLRDIAADDPTLEIHTQPNAGHGAAILNGYHRSADVDWVFQIDSDHQLD
                     TAAFTRLWANRQDYDLLIAQRQEKNATAGRQRISNISRVMVRMLFGNTVSDVNSPYRL
                     MRGERLREALERIPPRSFAPNILLTAWFVRKKSRIFTTTIQNRKER"
     gene            complement(678..2078)
                     /locus_tag="VKQ52_00060"
     CDS             complement(678..2078)
                     /locus_tag="VKQ52_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85589.1"
                     /translation="MSSLSAQSIYKLQADSVKIFNCPDSAELILENHTQGVPGFLFNT
                     GNGRTQFRRGMSTAGRGLYVFGADTLNLAGTAWLQGGNAFGTTGMLGTLDNNPMDFYT
                     NGTQQGRLTGAGNWLLGTTADNGAKLQVAGPTYMKAGSVPSFAFVAAGESSGRSAVFG
                     DSALSPADYNHKGLQVNVFPDRATIASLSNVQSAAPANLSLNEAGGKVLVGTASDNGY
                     GATLQVNGNSYVGQQLQVGNFVPGSTVASSGASVITQGQVRATQGFNLREPAAHDNEF
                     IGLAAGGVGVALEISSVPLFSVGFVQGASSGTQVSIASGFNPTTGSGTVNVLDFTASQ
                     IQMLSGSNSITYNFLNLSPAVNQGTYGTGISRGLYINPNLGAAADWRSIETVRGDNRL
                     NTASGNTGIGLNSAPTSKLDVSGAQGYSQLRLRTTYTPTSSSDANGNVGDFSWDGSYF
                     YIKTPDGWKRSALTTF"
     gene            complement(2148..2732)
                     /locus_tag="VKQ52_00065"
     CDS             complement(2148..2732)
                     /locus_tag="VKQ52_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85590.1"
                     /translation="MGLFYRASEKELLESRNRIFVEMGIPALQEKGFRRSPFSTSAYG
                     RNHLKDFSYEFCRLTERSSLEIIAIYLSRRDRWIKIDLNIFQLSPELPSLDRLGGLNG
                     LQFGQPPDSLTKMRLRDDDYKGSPLFTALFRREHRLGRYYSKGGLQRGLKALGELIER
                     DMTNIDRFIRRWHELHKPLVVTWEGHPVAPADAS"
     gene            complement(2734..>4475)
                     /locus_tag="VKQ52_00070"
     CDS             complement(2734..>4475)
                     /locus_tag="VKQ52_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85591.1"
                     /translation="GNITTYLRNGSSATSSTNMDNMTYSYLTGTNKLDHITDAVTTYC
                     ATCNDITNQSPGNYQYDSIGEMTADNAAGIANISWTVYGKVASITKSGDTTIQFTYDP
                     EGNRISKAVIHAGDVSTTWYVRDAQGNILSTYTSGDPAVNGKDLTQIELDIYGTSRLG
                     TWKRNVDVEMMTGSDSIALPLLGSGDSLIFTRGNKLFELVNHLGNVLSTISDKRFGVS
                     FDDSTVAYYNPEIVSANDYYPFGMLQPGRTYTETGAGTYRFGFNGQEGDNEINGRGGA
                     YSAKFWEYDPRIGRRWNIDPKSEEIHDVSPYLCLGDNPVLHDDPNGDFWHIIAGAIIG
                     AVIDAGIEAGSQLIKHGSITSWKAIGGAAVQGAITGGVAAATGGASLAVSATTKVVIH
                     VAAAAAANVVGGTLNRAVQGKKTTLADVGIDALAGAGGELAGKAVSKVAKGAVDKLSN
                     QAKGKLGETLTQLKYAAKGYVRKGNAVVQTGRLTRVTREAEKAVYDHDFKQVITGKRL
                     TVESKFNTSQLNIRQRLAQSKVQTVGGLIVDRTNSAQVSHVAKAATSNTVTGGTESVK
                     QAVTGGSGSTKQQ"
BASE COUNT          938 a         1321 c         1153 g         1063 t
ORIGIN      
        1 accgttcctt cctgttttgt attgtggtcg taaatatcct gctttttttg cgtacaaacc
       61 aggccgtcag gaggatgttg ggggcaaagc tcctgggggg gatacgttcc agggcttccc
      121 ggagccgttc tccgcgcatc agccggtagg gggagttgac atcgctgacg gtgtttccga
      181 agagcatccg gaccatgacc cgggagatat tgctgatgcg ttgccgtccc gccgtggcat
      241 tcttttcctg gcgctgggcg atgaggagat catagtcctg gcggttggcc caaagacggg
      301 taaaggccgc cgtatccaat tgatggtcgg aatcgatctg gaagacccag tcgacatcag
      361 cactccggtg gtagccgttc aggatggcgg caccgtgacc ggcgtttggc tgggtgtgga
      421 tctcgagtgt ggggtcgtcg gccgctatgt cccgcaggag ccgcaggcta tcatcccggg
      481 aaccgtcatc gatgacgatc acccggtagg gcgcccccgt cgaccggaac accggttgcc
      541 agtccctcag cagcgccgga aggttctccg cttcgttata gaccgggatg acgacggata
      601 atacgggctt ttgcggcatg ccgcaagata gtgcttaaaa cagacatatg ccagccggcg
      661 cctccggcgc cgccacacta aaaagtggtc aatgccgaac gcttccagcc atcaggggtc
      721 ttgatataga aatagctccc atcccaggaa aaatcgccca cattcccatt ggcgtcggac
      781 gaggaggtgg gagtataggt tgtacgcagc ctcagttgcg aatagccctg tgcgccggac
      841 acatccaact tcgacgtcgg cgcgctgttc agcccgatac cggtattgcc ggaggcggtg
      901 ttcagccggt tgtcccctct cacggtctcg atcgagcgcc agtcggccgc cgcgccgagg
      961 tttggattga tgtacaggcc gcggctgatt cccgtgccat aggttccctg gttcacggcg
     1021 ggcgagaggt tgagaaaatt gtaggttatc gaattcgaac cgctgagcat ctggatctgg
     1081 gaggcggtga agtcaaggac attcaccgta ccgctgccgg ttgttggatt gaacccgctg
     1141 gcaatgctta cctgcgtgcc gctcgaggct ccctgtacaa atccgacgga gaacagcgga
     1201 acagagctga tctccagggc gacgcctacc ccacccgcgg caagaccgat aaattcattg
     1261 tcatgtgcgg cgggctcccg gaggttgaac ccttgagtcg cccgtacctg tccctgggtg
     1321 ataacgcttg cgcccgatga tgccacggtg ctgcccggga caaaattccc cacctgcagt
     1381 tgctggccca cataactgtt gccgttcacc tgtaaggtcg ccccatatcc attgtccgag
     1441 gccgttccca ccagcacctt accccccgcc tcattcagcg acagattggc cggcgccgca
     1501 ctctgcacat tagacagcga agcgatcgta gccctgtccg ggaatacatt tacctgcagc
     1561 cccttgtggt tgtaatccgc cggcgacagg gccgagtcgc caaacaccgc cgatcgcccc
     1621 gaggattcgc ccgccgccac aaaggcgaag gaggggaccg atcccgcctt catataggtg
     1681 ggaccggcca cctgcaattt cgcgccgtta tccgccgtgg tgcccagcaa ccagttgccg
     1741 gcgcccgtca gcctcccctg ctgggtgcca ttggtataga aatccatcgg attattatcc
     1801 agcgttccca gcatccctgt cgtgccgaag gcattgccgc cctgcagcca tgccgtcccc
     1861 gccaggttca gcgtgtctgc gccaaaaaca tacagccccc tccccgccgt actcatcccc
     1921 cgccggaact gcgtccggcc attgccggta ttgaacagga aacccggcac cccctgggta
     1981 tggttctcca ggatcagctc cgcggagtcc ggacagttga agatctttac actgtccgcc
     2041 tgaagcttgt aaatggactg ggcagagagg gaagacataa gggctataac gcaaaaggaa
     2101 atcaggaaag gtttcataaa atccaagact gataaataat aaaacgatta agatgcatca
     2161 gcgggagcga cagggtgtcc ttcccaggtc acaaccagcg gcttatgcaa ctcatgccat
     2221 cgccggataa aacgatcgat atttgtcata tctctttcga tcagttcgcc cagggctttc
     2281 agcccccgtt gcagacctcc tttgctatag tatcggccta accgatgctc tcttcgaaaa
     2341 agggcagtaa aaaggggcga ccctttatag tcatcgtcac gcaacctcat ttttgtaagt
     2401 gaatctggag gttgcccgaa ttgcaacccg ttcagaccgc ccaagcggtc taatgatggc
     2461 aattccggcg atagctgaaa aatattcaga tcgatcttta tccaacgatc acgtctcgat
     2521 aggtatatgg caatgatctc aagcgaagat ctctccgtca atctacaaaa ttcatagcta
     2581 aagtctttaa gatgattgcg gccatacgcg gatgtcgaaa atggggaccg cctgaatccc
     2641 ttctcttgaa gagcagggat ccccatttcg acgaaaatcc ggtttcttga ttccaacaat
     2701 tctttctcag atgcgcgata aaacaatccc atactactgc tgtttcgttg atcccgagcc
     2761 gccggtgaca gcctgtttta ctgattcggt tccaccggta actgtattcg atgttgccgc
     2821 cttcgcaaca tgacttacct gcgcactatt cgttctgtcc acgattaatc ctcccacggt
     2881 ttgtaccttg ctttgggcca ggcgttgcct gatattcagt tggctggtgt taaatttact
     2941 ttcgacggtc agtctctttc ctgtaattac ctgcttaaaa tcgtgatcat agactgcttt
     3001 ttcggcttcc cgggtcactc gggtcagtct tccggtttgc accactgcat tcccctttct
     3061 aacataacct tttgccgcat atttgagctg tgtaagcgtt tcccccaatt ttcctttggc
     3121 ttggtttgaa agtttatcga cggcgccctt tgctactttg ctcacagctt tacccgccaa
     3181 ctctcctccc gctccggcca gcgcatcgat gccgacatct gctaaagtcg tctttttccc
     3241 ctggaccgcc ctgttcagcg ttccccccac cacattggca gctgccgccg cagcgacatg
     3301 tataactact ttcgtggtag cagaaaccgc caggcttgct ccgccggttg ccgccgcaac
     3361 cccaccggtg atcgcgccct gcactgccgc accgccgatc gccttccaac tggtgatgga
     3421 gccgtgcttg ataagctggg aaccggcttc aatcccggcg tcgatcaccg caccaataat
     3481 tgcccctgca atgatatgcc agaaatcgcc attggggtca tcatgtaaca ccgggttatc
     3541 ccccagacac aaatacggcg aaacatcatg tatttcttcc gatttggggt cgatattcca
     3601 cctccgcccg atccgcggat cgtattccca gaacttggcg gaataggcgc ctcccctgcc
     3661 gttgatctcg ttatctccct cctggccgtt gaacccgaac cggtaggtgc ccgcaccggt
     3721 ctcggtgtat gttcttcccg gctgtaacat cccgaacgga tagtagtcat tggcacttac
     3781 gatctcggga ttataatagg caacggtgct gtcatcgaac gaaaccccga atcgcttgtc
     3841 actgatcgtc gacagaacat tcccaaggtg attcaccagt tcaaagagct tgttcccgcg
     3901 ggtaaagata agactatccc cgcttcccag caggggcagc gcgatgctgt ccgacccggt
     3961 catcatctcc acgtcgacat tccgtttcca ggtgccgagt cggcttgtcc catagatatc
     4021 caattctatt tgtgtaaggt cctttccgtt caccgccggg tcgccactgg tataagtgct
     4081 caggatattg ccctgtgcat cacgcacata ccaggtggtc gatacatccc ccgcgtggat
     4141 cacggccttg ctgatccggt taccctcggg atcataagtg aattggatgg tagtgtcgcc
     4201 ggatttggtg atgctggcga cctttccata caccgtccac gaaatattgg ctataccagc
     4261 tgcattatcg gcggtcattt ccccaatgga atcgtattgg taattgcccg gcgactgatt
     4321 ggtaatatca ttgcaggtag cgcaataggt cgtcaccgca tccgtgatgt gatccagctt
     4381 attcgtaccg gtcaggtagg aataagtcat gttatccata ttggtcgacg aagtagcact
     4441 gctgccgttt cgcaaatagg tggttatatt accat
//
LOCUS       DATGFG010000005         4325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_684002, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000005 DATGFG010000000
VERSION     DATGFG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684002"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VKQ52_00075"
     CDS             complement(<1..592)
                     /locus_tag="VKQ52_00075"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HLZ85592.1"
                     /translation="MPAPPTSGLSPYTGPWTENEMAHLLKRCLFGASIADIAWFKTKT
                     LPATIAELLTPATTSPPPPVKEYTTPSSALNPDNNIAQGTTWVNDPNTDGAVDSVRRA
                     SFKKWWMGVMVNQDRSLLEKMTLFWHNHFSTETNTVGNSQYVYKHHAMLRTNALGNFR
                     TLVLQVTTDPAMLVYLNGQYNTAAAPDENYGRELQEL"
     gene            complement(682..3402)
                     /locus_tag="VKQ52_00080"
     CDS             complement(682..3402)
                     /locus_tag="VKQ52_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HLZ85593.1"
                     /translation="MRRLSLLLAASFCTFITWGQQQTAYSILSVPGASGYTHIDTTGI
                     SILPSGRYCTPVGQTITLTHDPFGMATSPDGATTVTLHDGVFTIIDNATLANTRVPSY
                     DGSIRSPFSKGSFLGAAFLPDSRTVYLSGGDNGAVIGYDLSTLQPIDSISLNGPNPTD
                     TVNYQESFTSDLLYNAGNNELLVLDRGNFRLVRIDLATKKITASIHTGRQPFGLAISP
                     DHKTAFEANVGLYDYPLLPDITPENYNSKLIPFHPYGDNTPQSREGYTWKDRKIPGVG
                     DPLAPEAMSVYAIDLSTNTITDRYKPGHQIGQMVEGAEVVGGASPNSLAIGSRFAYVT
                     NATNDNISVIDYRNHRITATIPIHVDPAIDRYRGLLPFGLTLSKDEKTLYVALLGFNA
                     IAVIDLPTGRTTGLIPTGWGPTRVLLGKDEHDLYVISCRGYGAGPNGGAHFIRPVQGT
                     YIGDIQLGTFQKIALPDSAQLATYTRQAIANTFSKTPVADDGRNPLPPLQHIRKSPIE
                     YIVYITKENRSYDEVLGQLPTGKGDTTLARFGIDVNLIAKDKTLHHMNIAPNHLKIAK
                     QFAFSDNYYCDSDASIHGHHWMLGVIPNEWVEANSSVDKTANIFSKASGRRFPGSTGS
                     MDPEDYAERGGLWEALERSHISFYNFGEANETAHVREEWMDTATGASHAVMVPMQKAL
                     WSRTSHNYAGFNTNVPDQFRMEQFESEFKKMWLDGKHKMPRLITMQVPNDHTAHPRPA
                     DGYPFIHSYVADNDIAIGRILHFLSRTPYWKKMLVIITEDDPQGGVDHIDAHRSILMM
                     AGPWVKHGYTSHAHANFGSILKTIYTIFDVPYVNQYDATASLLQDFFTGKPDFRPYDL
                     VFPSTEVFDPQVAMKRYNKTIDWRKIEKGPEMDDEKEQREEHYRQQHGLN"
     gene            3464..>4325
                     /locus_tag="VKQ52_00085"
     CDS             3464..>4325
                     /locus_tag="VKQ52_00085"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ85594.1"
                     /translation="MEGAWQVLIDNFTILRKRDMRIYLTGNAVSLVGDWMQQTAQAWV
                     VWQLTHNATALGVVAFFSQLPFFLFGPWVGVISDRYDRRKILLVTQFLIMSFAFILAF
                     LVESQYLRLWHVYLLAFLLGVVSAFDVTAEQAFIADIAGEGNIRKAIALNNSLTQLTR
                     LVGPALSGYLIAQIGVAPSFWINGLSILVCIACLWAIHSRGVQSEAKGKGLQQFREGW
                     RFFIRHPLLRLIVIFAGVQAFFGLSTIQLLPAIASVVMKGDANTLGRLLGAAGAGALV
                     GILVVMPFVER"
BASE COUNT          748 a          916 c         1564 g         1097 t
ORIGIN      
        1 acagctcctg gagttcgcgg ccatagtttt cgtcgggggc ggcggcggtg ttgtactggc
       61 cgttgagata gacgagcatg gccgggtcgg tggtgacctg cagcaccagg gtccggaagt
      121 tgcccagggc attggtgcga agcatggcgt ggtgcttgta gacgtattgg gagttaccga
      181 ccgtattggt ctcggtggag aagtggttgt gccagaaaag ggtcattttt tcgagcaggc
      241 tgcggtcctg gttgaccatt acccccatcc accatttttt gaaggaagcc cgtctgacgc
      301 tgtcgacggc gccgtctgtg ttcgggtcat tgacccaggt ggttccctgg gcgatgttgt
      361 tgtcgggatt gagggcgctg gagggggtgg tatattcctt tacggggggt gggggagagg
      421 tggtggcggg ggtcagcaat tcggcgatgg tggccggtaa tgttttggtt ttgaaccagg
      481 cgatgtccgc gatgctggcg ccgaacaggc aacgtttgag gagatgagcc atctcatttt
      541 ccgtccaggg gccagtgtat ggtgataatc cgctggtggg tggtgcaggc atagcaaaag
      601 agattagcaa ttagccatta tgataccgtg ccgggttaga cccaggggat ggggaaaggt
      661 ttaaagaaag tataattttt cttaattgag gccgtgttgc tggcggtaat gttcttcgcg
      721 ttgttccttt tcatcgtcca tttcggggcc tttctctatt tttcgccaat cgatggtttt
      781 gttgtaccgt ttcatggcca cctgcgggtc gaagacttcc gtcgaaggaa agacgagatc
      841 gtatggccgg aagtccggtt tgccggtgaa gaagtcctgc aggaggctgg cggtggcgtc
      901 gtactggttg acgtagggga cgtcgaagat ggtgtagatc gttttcagga tggaaccgaa
      961 gttggcatgg gcgtgtgagg tgtagccatg ttttacccat gggccagcca tcatcaggat
     1021 gctgcggtgg gcatcgatgt ggtccactcc gccttgggga tcgtcttcgg ttatgatgac
     1081 cagcatcttt ttccaatagg gggtgcggga caggaaatgg aggatgcggc cgatggcgat
     1141 gtcgttgtcg gcgacatagg agtggataaa gggatagccg tccgcggggc gggggtgggc
     1201 ggtgtgatcg tttggcacct gcatcgtgat caggcggggc attttatgtt tgccgtccag
     1261 ccacattttt ttgaattccg actcgaactg ttccatgcgg aactggtcgg ggacatttgt
     1321 attgaagccg gcgtagttgt gactggtgcg ggaccaaagg gccttctgca tcggcaccat
     1381 tacggcgtgg gaggcgcctg tggccgtgtc catccattct tcccgtacgt gtgccgtttc
     1441 attcgcttcg ccgaaattat agaaggagat atgactgcgt tcgagggctt cccagagtcc
     1501 gccgcgttcg gcatagtctt ccgggtccat gctacccgtg gaaccgggga agcggcggcc
     1561 ggaggctttg gagaagatgt tcgccgtctt gtcgacgctg gaattggctt cgacccattc
     1621 gttggggata acgccgagca tccagtggtg gccgtgtatc gaggcgtcgc tatcacagta
     1681 atagttatcc gagaaggcga attgctttgc gatcttgaga tggttggggg cgatgttcat
     1741 gtggtggagg gtcttgtcct ttgcgatgag gttgacgtcg atgccgaaac gcgccagggt
     1801 ggtgtcgccc ttgccggtgg gcagctggcc cagcacttca tcatagctgc ggttctcctt
     1861 tgtgatgtag acgatgtatt cgatggggct tttgcggata tgctgcaggg gtgggagggg
     1921 attgcggccg tcgtcggcga caggggtctt tgaaaaggtg ttggcgatgg cctggcgggt
     1981 ataggttgcg agttgggctg aatcggggag cgcgatcttt tggaatgtcc ccagctggat
     2041 atcgccgatg taggttccct ggacggggcg gatgaaatgt gctcccccgt tggggccggc
     2101 gccatagccg cggcaggaga tgacgtagag atcatgttcg tctttaccca gcaatacgcg
     2161 ggtggggccc cagccggtcg ggatgagccc ggtggtcctg ccggtaggga ggtcgatgac
     2221 ggcgatggcg ttgaagccaa gcagggcgac atagagtgtc ttctcatctt tgctcagggt
     2281 cagtccgaag gggagcaggc cacggtagcg gtcgatggca gggtcgacgt ggatggggat
     2341 ggtagcggtg atgcggtgat tgcggtagtc gatgacggag atattatcgt ttgtggcgtt
     2401 ggtcacatag gcgaagcggc tgccgatcgc cagggagttc gggctggcgc cccctactac
     2461 ctcggcgcct tccaccatct gaccgatctg gtggccgggt ttgtagcgat cggtgatggt
     2521 gttggtggac aggtcgatgg catagacgct catggcttcg ggggcgaggg ggtcgcctac
     2581 gccggggatc tttctgtctt tccaggtgta accttctctc gactgcggag tattatcccc
     2641 atagggatga aaggggatga gtttgctgtt ataattctcc ggggtgatgt cgggcaggag
     2701 ggggtagtcg tagaggccga catttgcctc gaatgcggtt ttgtgatcgg ggctgatggc
     2761 gaggccgaag ggttgtcttc cggtgtggat ggaggcggtg atcttttttg tagcgagatc
     2821 gatcctgacg agacggaagt tgccgcgatc gaggaccagc agttcgttgt taccggcgtt
     2881 gtagagcagg tcggaggtga agctttcctg gtagttgacg gtatcggtgg ggtttggacc
     2941 attcagggag atggagtcga tgggttgcag ggtggagaga tcgtagccga tgaccgcgcc
     3001 gttgtcaccg ccgctgaggt agaccgttct ggagtcgggg aggaaggccg cgccgaggaa
     3061 ggagcctttc gagaaggggg agcggatgga accgtcgtaa gagggaaccc gggtattggc
     3121 cagggtggca ttgtcgatga tggtaaatac gccgtcgtgg agggtgacgg tggtagctcc
     3181 gtcgggggat gtggccatac cgaaggggtc gtgggtgagg gtgatcgttt ggccgacggg
     3241 tgtgcagtag cggccgctgg ggaggatgct gatgcccgtc gtatcgatat gggtgtagcc
     3301 ggaggcgccg ggcacggaga ggatggagta agctgtttgt tgctggcccc aggtgatgaa
     3361 ggtgcaaaaa gaggcggcta ggaggagtga caggcggcgc atggcgttat tattattggg
     3421 taaaaatagc gtacaattat taccttagga ttaaatcagc aaattggaag gcgcctggca
     3481 ggtattgatc gataatttta cgatcctccg caagcgggat atgcgtattt atctaaccgg
     3541 gaatgcggtt agtcttgtcg gagattggat gcaacagacg gcgcaggcct gggttgtctg
     3601 gcagttgacg cacaatgcga cggcgcttgg ggttgtcgct tttttctcgc agctgccgtt
     3661 ctttcttttt ggcccctggg tgggtgttat ttccgatcgg tacgaccggc gaaagatcct
     3721 gctggtgacg cagttcctga tcatgtcgtt tgcttttatc ctcgcttttc ttgtggagag
     3781 tcagtatttg cggttgtggc atgtttactt gctggccttt ttgcttgggg tggtgtctgc
     3841 gttcgatgtg acggcggagc aggcgtttat cgcggatatt gccggggagg ggaatatccg
     3901 caaggcgata gcgctgaaca attccctgac gcaactgacg cggcttgtcg ggccggctct
     3961 ttcgggctat ttgatcgcgc agatcggggt agccccttct ttttggatca acgggctgag
     4021 tatccttgtc tgtatcgcct gtctttgggc catacattcg cgtggggtgc agtcggaggc
     4081 gaaggggaag gggcttcagc agttcaggga ggggtggagg ttctttatcc ggcatccgct
     4141 tttgcgactg atcgtgatct ttgcgggggt gcaggctttt tttgggcttt cgaccattca
     4201 gttgttgccg gcgattgcgt cggtggtgat gaagggagat gcgaatacgc ttgggcgatt
     4261 gttgggtgcg gcgggggcgg gggcgctggt tgggatcctg gtggtgatgc cgtttgtcga
     4321 acgga
//
LOCUS       DATGFG010000006         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_683524, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000006 DATGFG010000000
VERSION     DATGFG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683524"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /gene="pulA"
                     /locus_tag="VKQ52_00090"
     CDS             <1..1560
                     /gene="pulA"
                     /locus_tag="VKQ52_00090"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HLZ85595.1"
                     /translation="KRPPLAHATDIILYELHIRDLSMDTASGIGHKGKFLGLTETGTR
                     SPQGEATGLDHIRELGVTHIHLLPSFDFGFVDEKQPNAGYNWGYGPLNYDVPEGSYST
                     DPYDGRVRIREFKQMVKTLHDKGLRVVLDVVYNHTADVADSRFTQFFPGYFYRHNPDG
                     SYSNATGCQNETASEKAMMRKFMVESVAYWANEYHLDGFRFDLMGVHDLATMNAISDT
                     LHKIDPTIFIYGEGWDAGRSPLPEEKRALKKNMRGIPGVAAFSDDIRDAIRGGMADLH
                     AGGFVSGEAKLAESIKFGIVAATRHPQIDYAQVNDSKSPWALEPSQAITYASCHDDNC
                     LWDRLQLSNPAASEADLIRMDKLAAAIVLTSQGVPFIYAGEEMLRTKKGVANSYNSPD
                     SINAIDWARKTRYKEVVTYYEALIALRRHHPAFRMPSAAAIRKHLQFLAMPDPSMIGY
                     VLKDHANGDNWKSILVYLNGGRQPQRVTLPPGDWVPVATEAEVREKGIGRPQTGVITV
                     APTAITIFYQL"
     gene            1772..>3546
                     /locus_tag="VKQ52_00095"
     CDS             1772..>3546
                     /locus_tag="VKQ52_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ85596.1"
                     /translation="MKRKRLLAGIAILFALTVSSHLHAQPKNISGTVTDARDGTPLAG
                     VSVVPKGASSGTTTASDGTFHLTVNARTRILEFSAVGFGSQEVPVGDGPVRVSLVAGT
                     SAALGEIVVIGYGTVKKKDLTGSIATVTVKDFQQGSITSPEQLISGKVAGVSITPGGG
                     QPGSGSTIRIRGGASLNASNDPLVVIDGVPLSSVNLQGINPNDIESMSVLKDAAATAI
                     YGSRASNGVIIITTKRGKAGKPVINFVPQVSASYNPKEYPVLSAGQFRTLVKTSGSAS
                     QAALLGTASTDWQKQIYQTAITSDNNLNVAGSFAGIPYRVSGEFLDQTGVLKTDNLKR
                     EALSISLTPLLFDKHLKIDLNLHGSNTDSRTANTGAIGNAVSFDPTQPVRSGKGGFGG
                     YFEWTTNDTTPNSLANRNPLAQLEQYNAQNHALRSFGNIQLDYKLHFLPDLHANLNLG
                     YDKAKYDGTTRVPAYAAQSYNAIPNLRGQNNQYYNNYSNKVLEFYLNYGKDLKSIASN
                     INLIAGYGYYDNYFHNKNYASISAANDTIPGTTPTYPSSFSEVTLESYYGRLIYTFHD
                     RYILMGSVRTDGSSRFGPKYRWGVFP"
BASE COUNT          843 a         1038 c          954 g          711 t
ORIGIN      
        1 aagcgtccgc cgctggcgca tgcaaccgat atcattctct atgagctcca tatccgggac
       61 ctttcgatgg ataccgcgtc gggcatcggg cacaagggga agtttctggg tttgacggag
      121 accggcaccc gaagccccca gggggaggcc acagggcttg atcatatccg ggagctggga
      181 gtcacgcata ttcacctgct gccctcgttc gacttcggct tcgtggatga aaagcaaccc
      241 aatgccgggt acaactgggg atatggtcct ttgaattatg atgtgccgga aggttcctat
      301 tccacggacc cctatgacgg ccgggtgcgg atacgcgaat tcaagcaaat ggtgaagacg
      361 ctgcatgata aggggctgcg ggtagtactg gacgtcgtgt ataatcacac cgcggatgtg
      421 gcggactccc ggtttaccca attctttcca gggtactttt accggcacaa cccggatggc
      481 agctattcga acgccacggg atgccagaac gaaaccgctt ccgaaaaagc catgatgcgg
      541 aaattcatgg tagagtcggt ggcgtactgg gcgaatgaat atcatctgga cgggttccgg
      601 ttcgacctca tgggggtaca cgacctggcg acgatgaatg cgatatcgga cacgctgcac
      661 aaaatagacc cgacaatctt tatttatgga gaaggatggg atgcgggtcg cagcccgttg
      721 ccggaggaga agcgggctct caaaaaaaat atgcgcggga tacccggggt cgcggcgttc
      781 agcgatgata tccgggatgc gatcaggggc ggcatggcgg atctgcatgc cgggggattt
      841 gtcagtggag aggcaaaatt ggcagagagc atcaaattcg ggatcgtcgc ggctacgcgg
      901 catccgcaga tcgactatgc gcaggtgaac gattcaaagt cgccctgggc gttggaaccg
      961 tcacaggcga tcacctatgc ctcctgccat gacgacaact gtctatggga ccggcttcag
     1021 ctttccaacc cggcggcaag tgaagccgac ctgatccgca tggataaact ggcggcggcc
     1081 atcgttctca cctcgcaagg ggtaccgttc atttatgcgg gcgaagaaat gctccgaacc
     1141 aagaaaggtg tggccaactc ctataattcg cccgattcca tcaacgcgat cgattgggcg
     1201 cggaaaaccc gctataagga ggtggtcacc tattatgaag ctctcatcgc tttgcggaga
     1261 catcacccgg cgttccggat gccctccgcg gcagcgataa gaaagcacct gcaattcctg
     1321 gctatgccgg acccctcgat gatcggttat gtcctaaagg accacgccaa tggggacaac
     1381 tggaagtcca ttttggtgta cctgaacgga gggcgccagc cgcaacgggt gaccctgcct
     1441 cccggggatt gggtgccggt ggctaccgaa gcggaggtgc gggaaaaggg tatcgggcgc
     1501 ccgcagacgg gcgtaatcac agtggctccg actgccatta ctatctttta ccagttataa
     1561 actttaaatt ttccagcgcc tcttgaaaaa tagaaaactg atagtcaatc aactgaaaaa
     1621 aacgatcgtg caattttcag tatttaattg taaacagcta ggaaaaggca ctaaaggaat
     1681 attcctttgt atcagataaa tttaactatt tgcttgttaa tgtgtaaaat ttacacatta
     1741 tcgattattc gccaactaat tgctcgcata tatgaaaaga aaacgactgc tggcaggcat
     1801 agcgatactg ttcgcgctta ccgtttcctc ccatctccac gcccagccaa agaacatttc
     1861 cgggacggtt accgacgccc gcgacggaac cccgctggct ggagtctccg ttgtgccgaa
     1921 gggcgcttca tccggcacta ccaccgcctc tgacgggacc tttcacctga ccgtcaacgc
     1981 ccgtacccgc atcctggagt tctcggctgt cgggtttgga agccaggaag tcccggtcgg
     2041 cgacggaccg gttcgcgtta gcctcgttgc cggaacatcg gccgccctcg gcgagatcgt
     2101 tgtcatcggc tatggtacgg taaagaagaa agacctcacg gggtctatcg ccaccgtcac
     2161 cgtcaaagat tttcagcagg gatcgatcac ctccccggaa caactcatct cgggtaaggt
     2221 ggccggcgtc tcgatcaccc cgggcggcgg gcaaccgggc tccggcagca ccatccgcat
     2281 ccgcggcggg gcctccctca acgccagtaa cgacccgctc gtcgtcatcg acggggtacc
     2341 cctgagttcg gtcaacctgc agggcatcaa ccccaacgac atcgaaagca tgtccgtgct
     2401 gaaagacgcc gcggcgaccg ccatctatgg ctcgcgggct tccaacggcg tcatcatcat
     2461 aacgaccaaa cggggcaagg ccggcaagcc ggtgatcaat ttcgttcccc aggtgtcggc
     2521 ctcgtacaat cccaaagaat atcccgtgct gtcggcaggg cagttcagga ccctggtgaa
     2581 gaccagcggt tccgcctccc aggccgccct cctgggcacg gccagcacgg actggcagaa
     2641 acagatctac cagacggcca tcaccagcga caacaatctc aatgttgccg gcagcttcgc
     2701 gggcatcccc taccgggtat ccggcgagtt cctcgaccag accggggtgc tgaagacgga
     2761 caatctcaaa cgcgaagcgc tgtccatcag cctcacccca ctgctcttcg acaaacatct
     2821 gaagatagat ctgaacctgc atggctccaa caccgattcc cgcaccgcca acacgggcgc
     2881 catcggcaat gccgtctcgt tcgatccgac ccaaccggtc cgttccggca agggtggttt
     2941 cggcggctat ttcgaatgga cgacaaacga tacgacgccc aactccctgg caaaccgcaa
     3001 tccgcttgcc cagttggagc aatacaacgc acagaaccac gcgctgcgga gcttcggaaa
     3061 tatccagctg gactacaaac tgcatttcct tcccgatctg cacgcgaatc tcaacctggg
     3121 ctacgacaag gccaaatatg acggcaccac ccgcgtcccg gcctatgccg cccagtccta
     3181 caatgccatt cccaacctcc ggggccagaa taaccagtac tacaacaatt attcgaacaa
     3241 ggtgctggaa ttctatctca actacgggaa agatctcaaa tccatcgcga gcaatatcaa
     3301 cctcatagca gggtatggat attacgataa ctactttcac aacaagaatt atgcgtccat
     3361 cagcgcggcc aatgacacga tcccgggaac gacacccacc tatcccagtt ccttcagcga
     3421 agtgaccctg gaatcctatt atggtcgcct gatctatacc tttcacgaca ggtatatcct
     3481 gatgggttcg gtaaggaccg acggttcttc gcggttcggc ccgaaatacc gctggggcgt
     3541 gtttcc
//
LOCUS       DATGFG010000007         7362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1253712, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000007 DATGFG010000000
VERSION     DATGFG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253712"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="VKQ52_00100"
     CDS             complement(<1..2359)
                     /locus_tag="VKQ52_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HLZ85597.1"
                     /translation="MNRIINISLVCLLPFLTRAQETQKLFLSGTGNDHTVDWKFFCTE
                     GRNSGKWTTIPVPSNWELQGFGKYNYGLDKDSVRGHEKGLYKYSFTVPAAWKGRVVDI
                     VFDGSMTDTEVKINGKSAGATHQGSFYRFRYNITGLLRYGSSNLLEVTVAKESTDHSV
                     NMAERHCDFWIFGGIYRPVYLEAMPVQHITSQAIDAKANGFFQASVHLSGITGADRLT
                     AQVYTLNGEKLGDAFSTKLYIGDTLAHLQANFTSPLTWTPETPHLYKVVYTLSKDNTA
                     IHTVEQRFGFRTVELREHDGIYVNGVKIKFKGICRHSFWPTSGRALSKALSLEDVGLI
                     KDMNMNAVRMSHYPPDDHFLDACDSLGVFVLDELTGWHHAYDTEVGSRLVKEMIEKDV
                     NHPSIVIWDNGNEGGFNFDLDPLFDKYDPQGRPLIHPWALFRHMDTQHYINYDYGNGT
                     YWHGHEVVFPTEFLHGLYDGGHGAGLYDFWELMWHEPLSAGGFLWDFSDEAVVRTDRN
                     GELDTDKDHGPDGILGPYREKEGSFFAVKEIWSPVRIQRREITPAFDGTFTVENRFFY
                     TNLHQCHFSWRLSHATTNPAGAAGEIASPDIRPGQIGTLALALPAHWQDFDILSLTAK
                     DPGGREIYTWTWPIAKPATIARRLVDTTGATAVKITQTDSLYTLNAAGITASFGKATG
                     MLRSIDNTKGAIPFTNGPTFTSGIATPQQVTTRMEGNTAILEAIYAKQSICQLLRWSM
                     YPSGWLKMEVHYFPPQYEYDLLGITFSYPEKGRDSVKAIRWLGNGP"
     gene            complement(2356..5610)
                     /locus_tag="VKQ52_00105"
     CDS             complement(2356..5610)
                     /locus_tag="VKQ52_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HLZ85598.1"
                     /translation="MTTRLTISLTLLLAAHLTALAWQHPAPVPGVEQPISPTAVTHVG
                     GFAGNRIEKNKDHYLKTFDIAKYVGLMETRTFTGWDWRQGEQPGKWLESAILSGDPGL
                     EKEARLMLGRLIRSQAPDGYIGITSPTVRTSEKPLGGMDAYELYFLQHALLTAYEQWK
                     DRQALTAAKRLGDYFVHYIGPGKAEFWPSPIHYPENREKTLKGTMHSDIAGHSIHYSW
                     EGTLLIDPMMRLYQLTGDKRYYGWCKWVVNSIDKWSGWDAYSNLDKVAAGTMPIDKIQ
                     PYVHAHTFQMNFLGLLRMYRVSGDSSYLRKVEGAWNDIASRQLYITGGVSVGEHYERD
                     FVKPLTGEMMETCATMSWMQLSQELLDIIGDPKYADAIERIQWNNVFAEQTIDGDANH
                     YFTPPNGYTPKGYYREPDCCMSSGERLLSMLPGFIYAGGAQDNFYINQFIPSTTTIGK
                     ITVIQQTNFPESGAITILLSAATPTKATINIRIPAWCTKPALAVNDQPVAVQPGTYAR
                     ITRSWRKGDKITLDLPLQLQWVRHDHYQQTADRKPYKTTPDDDAPYALVRGPVVYAVD
                     DIWYNGDTADFHRGWMDSVRYLLTSPAQLKSIPAPDSNILGPGYELTLMLSNGNKANL
                     PVYPFANIGKWYRDPAHKPDSNAAAWTYAIWLKGQPANNGALKMPHIFGDNMVLQREA
                     PVPVWGNARPGATVTVSFNDQRKTTTTDPQGHWRLTLDPLEASANPATLLVSSEDGSI
                     QYRNILVGEVWLCSGQSNMEYPMRRIKQPDTANNPDIRIFLVRNNYSKPDEKNIRRVW
                     DPAIGTPLRDFSAPAYFFAKELYATLHIPIGVISNAIPGSRIEPWLPDTGKFYTNIVH
                     PLAPYAIRGFCWYQGESNCFLSDTLAYAAKMTTLINSWRHLWETPAASFYLVQITPYE
                     YSKTTHSVFTEPAFWEAQQKALTLPHTGMIVTTDLVDSITQLHPMDKWTVGHRLALLA
                     LANDYHQQLTANSPTFQSMSIKGRTIQVKFINIGGGLVSHDGQAIDWFETADKNGKFT
                     PATATITGPDTVTLTAKNKPIAVRFGWSEAAQPNLYNKEGLPAMPFRASANQIK"
     gene            complement(5607..>7362)
                     /locus_tag="VKQ52_00110"
     CDS             complement(5607..>7362)
                     /locus_tag="VKQ52_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85599.1"
                     /translation="IVHYSGHTLSNPDYHHGQLTPAVGVHNMQVLRANREHPEPAEGS
                     GFTYNHAPMLAYWNNKFYLEYLSDRVGESVPPGQTLLLTSPDGYTWSRPIVIFPPYKI
                     PDGTTKEGHPGVAKDLYAVMHQRMGFYVSKHNRLLVLGFYGICLDAHDDPNDGKGIGR
                     VVREIRPDGSFGPIYFIHYNPAWNSKNTTYPFYTESKDKNFITDCNELMATPLMMMQW
                     VEETDRNDPLIPLHKDYKAFNFYHLPNGHVIGLWKNGLTGFSTDEGRTWSRVIRAPHI
                     VTSNAKIWGQRTSDGNYATVYNPSEFRWPLAISTSKDGLDYTNLLLVNGEISTARYGG
                     NYKNYGPQYTRGIEEGNGTPPDGNIWITYSMNKEDIWVSTIPAPVTDKVTEDVNDVFA
                     QMPKGKELEHWNTYSPLWAPVSITANGLELKDTDPYDFAMAERVFPVAKKMTAEFAVT
                     PAQADHGQLQIEFQDERNAPAVRLSFEPDGTLIAKAGARVRKLLAYKAGLSYTLKVSL
                     NTDTRMYTVNIDGKDVMTQLFFAPVESLGHIVFRTGEARHFPTADSPAEADTDQPNAG
                     TPVPAAIFTIQYLKTTKQ"
BASE COUNT         1367 a         1708 c         2480 g         1807 t
ORIGIN      
        1 aggggccgtt gcccagccag cggatggctt ttacggaatc ccggcctttc tccggatagg
       61 agaaggtgat gccgagcaga tcgtattcgt attgcggcgg gaagtaatgg acttccattt
      121 ttagccagcc gctggggtac atgctccatc gcagcaattg acagatgctt tgctttgcat
      181 agatagcttc cagtatcgcg gtattgccct ccatccgggt ggtcacctgt tgaggggtgg
      241 cgataccgga ggtgaaagtt ggtccattgg taaaggggat cgcacctttt gtgttgtcga
      301 tgcttcggag catgcccgtg gctttgccaa aagacgcggt gatcccggcg gcgtttagtg
      361 tatagaggga atcggtttgg gttattttta ccgctgttgc gcccgttgta tcgacgaggc
      421 ggcgggcgat ggttgcgggt tttgcgatcg gccaggtcca ggtgtagatc tcgcgtccgc
      481 cgggatcttt tgccgtcagc gagaggatat cgaagtcctg ccaatgagcg ggcagtgcga
      541 gggcgagggt tccgatctgg ccggggcgga tatccgggga cgcgatctcg cctgcggcgc
      601 ctgcgggatt ggttgtcgca tggctgagcc gccaggagaa atgacactgg tggaggttgg
      661 tatagaagaa ccggttctcg acggtgaacg taccgtcgaa tgccggggtg atctcgcggc
      721 gttggatgcg cacgggggac cagatctctt ttacggcaaa gaagctgcct tctttctcgc
      781 gatagggtcc gaggatgcca tctgggccat ggtccttatc ggtgtcgagt tcgccgttgc
      841 gatcggtgcg aacgacggcc tcgtcggaga agtcccagag gaagccgccc gcggataggg
      901 gttcgtgcca catcagctcc cagaaatcat aaagccccgc gccatgaccg ccgtcataga
      961 gaccatggag gaactccgtc gggaagacga cctcgtgtcc atgccagtat gttccgttgc
     1021 catagtcata gttgatatag tgttgggtat ccatgtggcg gaatagggcc cagggatgga
     1081 tcagcggccg gccctgggga tcgtatttat cgaacagggg atcgaggtcg aagttgaacc
     1141 cgccttcgtt gccattgtcc cagatgacga tggaggggtg gttgacgtct ttttcgatca
     1201 tctccttcac cagcctcgac cctacttccg tgtcataggc atgatgccag cctgtcaact
     1261 cgtccagtac aaagacaccg agggagtcgc aggcgtcgag gaaatgatcg tcgggcgggt
     1321 agtgggacat gcgtacggcg ttcatgttca tatcctttat caggccgacg tcttcgaggc
     1381 tcagggcttt gctcaaggcg cggccggagg tcggccagaa ggaatggcgg cagatgcctt
     1441 tgaattttat cttcaccccg ttgacataga tgccatcgtg ttcacgtagc tctacggtgc
     1501 gaaagccgaa ccgttgttcg accgtatgga tcgcggtgtt gtcttttgaa agggtataga
     1561 ccactttata caggtggggt gtctccgggg tccaggtgag cggagacgtg aaattggcct
     1621 gcaggtgagc gagggtatcg ccgatgtaga gttttgtgga gaaggcgtcg cccagttttt
     1681 caccgttgag ggtatagacc tgggccgtaa ggcggtcggc gcccgtgatg ccggaaagat
     1741 ggacggatgc ctggaagaag ccgtttgcct ttgcgtcgat ggcctggctt gtgatgtgct
     1801 ggacgggcat ggcttcgagg tagacggggc gatagatgcc gccgaagatc cagaaatcac
     1861 aatgccgttc ggccatgttc acggaatggt cggtcgattc ctttgctacg gttacctcga
     1921 ggaggttgga gctgccgtaa cggagaagac cggtgatgtt atagcgaaag cggtaaaagg
     1981 aaccctggtg ggtggcgccc gctgacttgc cgtttatctt tacctcggtg tcagtcatgc
     2041 tgccatcgaa gacgatatcg accacccggc ctttccaggc tgcgggaaca gtgaagctgt
     2101 atttgtagag gcctttctcg tgaccacgga cggaatcctt gtcgagtcca tagttgtatt
     2161 taccaaagcc ctgtagttcc cagttggagg gtacggggat ggtggtccat ttgccggaat
     2221 tgcgcccctc ggtacaaaag aatttccagt ctacggtgtg gtcgttaccg gtacccgaga
     2281 ggaagagctt ttgggtctcc tgtgcgcggg tgaggaaggg cagtaagcag acgagcgata
     2341 tgttgatgat ccggttcatt tgatttggtt ggcggaagcc cggaacggca tggcgggcaa
     2401 gccttctttg ttgtataaat tcggttgagc cgcttcactc cagccaaagc ggacggcgat
     2461 gggtttattc tttgccgtta gggtgaccgt atcggggccg gtgatagtcg ccgttgcggg
     2521 agtaaattta ccgtttttat ccgcagtttc gaaccagtcg atggcttggc cgtcatgact
     2581 gacaaggccg cctccgatat tgatgaattt tacttggatg gttcggcctt tgatgctcat
     2641 ggattggaag gttggggaat tggcggtcag ctgctgatga tagtcgtttg cgagggcgag
     2701 aagggcaagg cggtggccga cggtccattt atccatcggg tggagctggg ttatgctatc
     2761 taccagatcg gtggtaacga tcatgccggt gtggggcagg gtcagcgctt tttgctgggc
     2821 ttcccagaat gcgggttcgg taaataccga gtgggtggtt ttggaatatt cgtagggggt
     2881 tatttgaacc aggtagaacg aggcggcggg tgtttcccag aggtgccgcc aactgttgat
     2941 cagggtggtc atctttgccg cgtatgcgag ggtgtcactg agaaaacagt tcgattcacc
     3001 ctggtaccaa cagaagcctc tgatcgcata gggggcgagg gggtggacga tgttggtgta
     3061 aaatttcccg gtatccggga gccagggttc tatcctgctg ccggggatgg cgttggagat
     3121 gacgccaatg ggaatatgga gggtggcgta tagttccttt gcaaagaagt aagcgggggc
     3181 ggagaagtct cttaacggtg tgccgatcgc ggggtcccag acgcgacgga tatttttttc
     3241 gtcgggtttg ctgtagttgt tgcggacaag aaagatacgg atatcgggat tattggcggt
     3301 atccggttgt ttgatccggc gcatggggta ttccatattg gactggccgc tgcagagcca
     3361 gacctcgccg acgaggatgt tgcggtattg aatactgccg tcttcgctgc tgacgaggag
     3421 ggtggccggg ttagccgagg cttccagagg atcgagggtg aggcgccagt gtccttgcgg
     3481 atcggtggtg gtggtttttc gctgatcgtt gaacgacacg gtgacggttg ccccggggcg
     3541 ggcgttgccc cagacgggaa cgggggcttc gcgttgcagg accatattgt cgccaaagat
     3601 atggggcatt tttagcgcgc cgttgttggc gggttggcct ttcagccaga tggcataggt
     3661 ccaggcggcg gcattgctat cgggtttatg cgccgggtca cggtaccatt tgccgatatt
     3721 ggcgaagggg tagacgggaa gattggcctt gttgccgttg gacagcatca gggtgagttc
     3781 gtagccgggg ccgaggatat tgctatcggg ggccgggatg gactttagtt gtgccgggct
     3841 ggtaaggagg tagcgaacgc tatccatcca acccctgtga aagtcggcgg tatcgccatt
     3901 gtaccagata tcatccacag catagacgac ggggccgcgg acaagggcat agggagcgtc
     3961 gtcatcggga gtcgttttgt atggttttct gtcggcggtc tgttggtagt gatcatgtct
     4021 tacccattgc agttgcaggg ggaggtcgag ggtgatcttg tcacctttac gccagctgcg
     4081 ggtgatgcgg gcgtaggtac ccggctgaac ggcgacgggt tgatcgttga cggccagggc
     4141 cggcttggta caccaggcgg ggatacggat attgatggtc gctttggtgg gagttgccgc
     4201 gcttaagagg atcgtgatcg cgccggattc cgggaagttt gtttgctgga tgacggttat
     4261 cttaccgatg gtggttgtcg aggggatgaa ctggttgata tagaagttgt cttgcgcacc
     4321 gccggcatag atgaagcccg gtagcatcga cagcagccgt tcgccgctgc tcatgcagca
     4381 atcgggttcg cggtagtagc cctttggggt atagccgttt ggcggagtga aataatggtt
     4441 tgcgtcgccg tcgatcgttt gttcggcgaa gacattgttc cactggatgc gttcgatcgc
     4501 gtctgcatat tttgggtcgc cgataatgtc gagcagttcc tgtgagagtt gcatccagga
     4561 catcgtggcg caggtttcca tcatttcccc tgtcaggggt tttacaaaat cccgctcata
     4621 gtgttcaccg acgctgacgc caccggtgat atatagctga cgggaggcga tatcgttcca
     4681 ggcgccctcg acttttctca gataggagct gtcgccgctg acacgataca tccgcaacaa
     4741 cccgaggaag ttcatctgga aggtatgcgc atgtacgtag ggctggatct tgtcgatggg
     4801 catcgtgccg gcggccacct tgtcgagatt ggaataagcg tcccagccgc tccatttgtc
     4861 gatgctgttc accacccatt tgcaccagcc gtaatagcgt ttatcaccgg tgagctggta
     4921 aagccgcatc atggggtcga tgaggagggt cccttcccag gaataatgaa tgctatgtcc
     4981 ggcgatgtcg gagtgcatgg tgcctttcag cgtcttttcc ctgttctccg ggtagtggat
     5041 gggtgagggc cagaattcgg ccttgccggg accgatatag tgtacgaaat agtcgccgag
     5101 gcgtttggct gccgtcaggg cctgccggtc tttccattgt tcgtaggccg tcagcagggc
     5161 atgttgcagg aaatagagct cataggcgtc catgccgccg agtggttttt ccgaggtgcg
     5221 aacggtaggg ctggtgatgc cgatatagcc gtcgggggcc tgtgatctta tcaatctccc
     5281 gagcatgagg cgggcttctt tttcgagacc ggggtctccg ctcaggatcg cggattccag
     5341 ccatttgccg ggttgttccc cctggcgcca gtcccagccg gtgaaagtcc gggtctccat
     5401 gaggccgacg tatttggcga tgtcgaaggt cttgaggtaa tggtctttgt tcttttcgat
     5461 gcggttgccg gcgaagccgc cgacatgggt gacggccgtg ggggagatgg gttgttcgac
     5521 accggggaca ggtgccggat gctgccaggc gagggcagta agatgggcgg cgagcagcag
     5581 cgtaagcgat atggtgaggc gcgtcgtcat tgttttgtcg tcttcaggta ttggatggtg
     5641 aagatagcgg cggggacggg ggtgccggcg tttggctgat cggtatcggc ttccgcgggg
     5701 ctatctgccg ttggaaagtg ccgggcttcg ccggtgcgga agacgatgtg gccaaggctt
     5761 tccacggggg cgaagaagag ttgggtcatc acgtctttgc cgtcgatgtt gacggtatac
     5821 atccgggtgt ctgtattgag gctgaccttc agcgtatagg agagccctgc tttataggcg
     5881 agtaatttcc tgacgcgggc gccggccttg gcgatgagtg ttccatcggg ttcgaagctg
     5941 aggcgaacgg ccggagcatt gcgctcgtcc tggaattcaa tctgcagttg accatggtcg
     6001 gcctgggcgg gagtgacggc gaattccgcg gtcatttttt ttgcgacggg gaagacgcgt
     6061 tccgccatgg cgaagtcgta ggggtcggtg tcttttaatt ccaggccgtt ggcggtgatg
     6121 gagacggggg cccagagggg gctataggtg ttccagtgtt cgagttcctt tccttttggc
     6181 atttgggcga agacatcatt cacatcctct gttaccttgt cggtgacggg tgcggggatc
     6241 gtactgaccc agatatcctc cttgttcatg ctataggtga tccagatgtt cccatcgggc
     6301 ggggttccat tcccttcttc gatgccacgc gtgtattgcg gtccgtaatt cttatagtta
     6361 ccgccatagc gggccgtgga gatctcgccg ttcaccagca gcaggttggt gtagtcgaga
     6421 ccgtccttgc ttgttgatat ggccaggggc cagcggaatt cggaggggtt atagacggtg
     6481 gcatagttac cgtcggaggt gcgttgaccc cagatcttgg cgttgctggt aacgatgtgg
     6541 ggggcccgga tgacgcggga ccaggtgcgg ccttcgtcgg tactaaagcc cgtgagcccg
     6601 ttcttccaga ggccgatgac atggccgttt ggaaggtgat agaagttgaa ggccttgtaa
     6661 tctttgtgca ggggtatgag gggatcgttg cgatcggtct cttccaccca ttgcatcatc
     6721 atgagcggag tcgccatgag ttcgttacag tcggtgataa agttcttgtc tttgctttcc
     6781 gtataaaatg gataggtggt gttcttactg ttccaggcgg ggttgtaatg gatgaagtag
     6841 atagggccaa agctgccgtc aggcctgatc tcgcggacca cgcggccgat gcctttgcca
     6901 tcattgggat cgtcatgcgc atcgaggcag ataccgtaga agccgaggac cagcagccgg
     6961 ttgtgtttgg aaacatagaa gcccatacgc tggtgcatga cggcatagag gtcctttgca
     7021 acaccggggt gcccttcctt cgtggtaccg tcggggatct tgtaaggcgg aaaaatgaca
     7081 ataggcctcg accatgtgta gccgtcgggc gatgtgagca ggagggtctg accgggggga
     7141 acgctctccc ctaccctgtc gctaagatat tcgagataga atttgttgtt ccagtaagcg
     7201 agcatcgggg cgtggttgta ggtaaacccc gatccttcgg caggctcggg gtgctcgcgg
     7261 ttggcgcgga gcacctgcat attgtggacg ccgacggcag gggtgagttg tccgtgatga
     7321 tagtcggggt tggaaagggt gtgcccgcta taatgaacga tg
//
LOCUS       DATGFG010000008         4024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1484215, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000008 DATGFG010000000
VERSION     DATGFG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484215"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VKQ52_00115"
     CDS             <1..409
                     /locus_tag="VKQ52_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ85600.1"
                     /translation="RDNLLTIQSFRPFIFRLARNRVIDWYRKNESKKSYCTFYSENYG
                     AASVSVTDDLLFEEYYAIAMEAIGKLSSRQKQIFNLRHTEDLSLIEIAEKLRISVHAV
                     KKQLYQATRFVREYLHKHGEWLVSMPFLLFFGN"
     gene            457..1659
                     /locus_tag="VKQ52_00120"
     CDS             457..1659
                     /locus_tag="VKQ52_00120"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ85601.1"
                     /translation="MPTETTRELIFKIATGNYTNEELTSFLDAVKIMDKASFIEAYQL
                     LYEEINKYPAEAVTPGFQDQLERRLDLLEAEKEFLPGNLASAPPKNKPVLLRSKWWNY
                     AAAAAILLFSVAGYLLLTTTKKPPPVAAQPVKKDIPAGRQGAILTLSTGQRIVLDSAA
                     NGVLTQDAGVRVIRKDGELSYGGNADQVLYNQVVTPRGRKWQLTLSDGTKVWLNAASS
                     VRYPISFVGRERLVEVSGETYFEVAPDKNRPFKINIAGKGAIEVLGTRFDVNAYDDED
                     AIRTTLLEGRVKVTRGKNSQLLSPGQQAVLGNGTDGIKVIPEANIDEVMAWRNGRFIF
                     SDMDLKSIMRQLTRWYDVDVVYEGRVPEIRIGGIIHNDVYLSTVMEFLGENGVHYKIE
                     GKKITIMQ"
     gene            1923..>4024
                     /locus_tag="VKQ52_00125"
     CDS             1923..>4024
                     /locus_tag="VKQ52_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ85602.1"
                     /translation="MRITAIFILGVSLHVSANSFSQSITISAKKITIEKAFRLIEQQT
                     DFSFLWNESVLDKSRVISIDVKDAPLSTVLDICLQGMPLSYRIMNKVVLIQLTRVIRI
                     EDSIRSALHPLADVHGRVLNENGEPLAGATVVIRGTTITALTDEQGNFHINTGDQVNP
                     VLQISFVGYLTEQYPLRGRDNFTIRLRQDTRSLGDVVIVGYGEQRKRDVTGAISTVKS
                     EEIAKRPIVRVEQALQGTTPGVAVQSANGMPGTPLSVRIRGVNSITGSNEPLYVIDGY
                     IGANLASISPSDIESLEILKDASATAIYGSRASNGVVIITTKTGHDGAPLLNAGAWLQ
                     KDEMPKYLALMNAYDFARTVNTQIASTSTAPPAFTDAQLAGFKANPGTNWQKAATQKP
                     LVQNYEASISGGSPHSKYFFAFNYLDQPGLLLNQYYKRATLRSNLDFKVKDKLDLKFS
                     VVIAVPASRNTAYVGDSGDPFASAVLFDPTAPVRNPATGQLTIQSAYGTLSVSPVAQE
                     SNQQVDANSADVLGTGVIKYHLLKNLTFTSSNSYETTYGWNRQFFGLNTSQTVVNGVT
                     AGYAQANSTWFHALQTSNFLTYNLHLGDHALTLLGLFEYQKGTNQNINARSANLSSYA
                     LGYYNLGLGQSQQTTSGYSSSDLQSFMGRVNYSYKDKYLLTASIRDDGSSVLTKKYSA
                     FPSVALGWNIAREHFMDNS"
BASE COUNT         1051 a         1082 c         1026 g          865 t
ORIGIN      
        1 acgggataac ctgttgacta tccaatcttt ccggcctttt atttttcgcc tggccaggaa
       61 cagggtgatc gattggtaca gaaagaatga atcgaaaaaa agctattgca cattttatag
      121 tgaaaattac ggggccgcgt cggtttccgt aacagacgac ctactctttg aagaatacta
      181 cgccattgcg atggaggcaa tcggcaaact ctcctcccgc caaaagcaga tctttaacct
      241 ccggcatacc gaagaccttt ccctgatcga gatcgccgaa aagctgcgaa tcagcgtaca
      301 tgcggtgaaa aagcaactgt accaggccac ccggtttgta agggaatatc ttcacaagca
      361 tggagaatgg cttgtttcca tgcctttttt gctttttttt ggaaactgag gtatactttc
      421 tccctgtccg tacgaaccta tccataatac cgaattatgc caacagaaac gaccagggaa
      481 ctgatcttca aaattgccac cggaaattat acgaatgagg aattgacgtc ttttctggac
      541 gcggtaaaaa tcatggataa ggcttctttt atcgaagctt accagttgct ctacgaggaa
      601 atcaataaat atcccgctga agcagtaaca cccggcttcc aggaccagct ggaaaggcga
      661 ctcgatctac tggaggctga aaaggaattc ttaccaggaa acctggcatc ggcgcctcct
      721 aaaaacaaac cggttctgtt gagaagcaaa tggtggaatt atgccgctgc ggctgctatc
      781 ctcttgtttt ccgtggccgg gtaccttttg ttgactacga ctaaaaagcc cccacccgtg
      841 gcagcacaac cggtgaaaaa agacataccg gcaggacggc agggagcgat actcacttta
      901 tccactggtc aacggatcgt cctcgacagc gccgccaacg gagtattgac acaggatgcg
      961 ggggtgcgcg tgatccggaa agacggagaa ctttcttatg gcggaaacgc ggatcaggtc
     1021 ctgtacaacc aggtggttac tccaagggga agaaaatggc agttgacgtt gagcgatggc
     1081 acgaaggtct ggctaaacgc cgcatcttct gtccgttatc ctataagttt tgtgggcagg
     1141 gagcgtctgg tggaagtcag cggcgaaacc tactttgaag tggcccccga taaaaacagg
     1201 ccgttcaaaa taaatatcgc cggaaagggg gctatcgagg tgctgggcac ccgcttcgac
     1261 gtcaacgcct acgacgacga agatgcgata aggaccacgc tgctggaagg cagggtaaag
     1321 gtgacccggg gtaaaaacag ccagttgctc agcccggggc agcaggccgt actcggcaat
     1381 ggaacggacg gtatcaaggt catacccgag gccaatatag acgaagtgat ggcatggagg
     1441 aacggccgtt ttatcttcag cgatatggac ctgaagtcca tcatgcgcca actaacacgc
     1501 tggtatgatg tggacgtggt gtatgaaggc agggttccgg agatccggat cggcggcatt
     1561 attcacaacg acgtgtacct gtccaccgtc atggagttcc tgggcgagaa tggcgtacac
     1621 tataagatag aagggaaaaa gatcaccata atgcaataac ccaaacacca tcgggcggcg
     1681 acgccccatt ttataaccaa aaacaaacta tgggcaacta accaatttct aacgatgctc
     1741 atgcccaaaa agaaccggag ccagattgcg cctgaccccg ggagatcggg tcatgaatga
     1801 accactcggt atcaatcaga atgtttcact ttaaaaccca acctgacaaa agtatgaaat
     1861 gtactttaca atgcaaagcc gttcccggaa gaggctcatt gctaacgaaa ttgctgcttg
     1921 ttatgagaat cactgctatt tttatccttg gtgtctctct gcacgtgagt gccaatagct
     1981 tttctcaaag cattaccatt tctgccaaga aaattaccat cgaaaaggcc ttccggttga
     2041 tcgaacaaca gacggacttt tcttttctat ggaacgaaag tgtcctggac aaatcgcgcg
     2101 tcatctccat cgacgtaaag gacgctccat tatccacggt gctggatatc tgtcttcagg
     2161 gcatgccctt gtcctaccgg atcatgaaca aggtcgtgct catccaatta acccgggtca
     2221 tccggatcga agacagcatc cgctccgccc tgcacccgct ggccgatgtt catggaaggg
     2281 tcctgaatga aaatggcgaa cctctggcag gagcaacggt cgtgatcagg ggaacgacta
     2341 tcaccgccct gaccgacgag caagggaact ttcatataaa caccggtgac caggtcaacc
     2401 cggtgctgca aatcagcttc gtaggctatc ttacggaaca gtacccctta agagggcgcg
     2461 ataattttac gatcaggctc cggcaggaca cccgatcatt gggagatgtg gtgatcgttg
     2521 gttatggcga acaacggaaa agggatgtga cgggcgccat cagcaccgta aagtcggagg
     2581 agatcgccaa gcggccgatc gtaagagtcg agcaggcgct gcagggaacc acccccggag
     2641 tagccgttca gagcgccaac ggcatgccgg gaaccccgct gagcgtccgg atcaggggag
     2701 tgaactcgat caccggttcc aacgaacctc tttatgtgat cgacggctat atcggggcaa
     2761 atctcgcatc catcagccct tcggatatcg aatccctgga gatcctgaaa gatgcgtctg
     2821 ccacggctat ttatggttca agggcatcca acggggtcgt catcatcacc acgaagaccg
     2881 gccacgatgg ggctcccctc ctcaacgcag gcgcctggtt acagaaggat gaaatgccca
     2941 aatacctggc cctgatgaac gcatacgatt ttgccaggac cgtcaacacc cagatagcat
     3001 ccacttctac cgcaccgccg gcctttaccg acgcccagct ggccggcttt aaggccaacc
     3061 cgggaacgaa ctggcagaag gcggcgaccc agaagccgct ggttcagaat tatgaagcgt
     3121 ccatttccgg cggctcgccc cactcgaaat atttctttgc ctttaattac ctcgaccagc
     3181 cgggccttct cctgaaccaa tattataaaa gggctacttt acgatccaac ctggatttta
     3241 aggtgaagga taaattggac ctgaaattca gtgtggtcat cgccgtgccg gccagccgga
     3301 atacggccta tgtcggggat agcggagatc cgttcgcctc ggccgtattg ttcgatccca
     3361 cggcgcctgt caggaacccg gccaccgggc aactgaccat ccaatccgcc tatggcaccc
     3421 tgagcgttag cccggttgcc caggagagca accagcaggt ggacgcgaac tccgccgatg
     3481 tgctcggcac cggggtcatc aaatatcatc ttctcaagaa ccttaccttc acttcctcca
     3541 actcgtatga aacaacgtat ggatggaacc gccagttctt tgggctgaat accagccaga
     3601 ccgtggtcaa cggggtcacg gcggggtatg cccaggcaaa ttcaacctgg ttccatgccc
     3661 tgcagaccag taacttcctg acctataatc tgcacctggg cgatcacgcc cttacgctcc
     3721 tgggcctgtt cgagtaccag aaagggacca accagaacat caatgcccgg tcggcaaacc
     3781 tgtcctccta tgcgctggga tattataacc ttggcctggg ccagtcgcaa caaacgactt
     3841 cgggatattc ttccagcgat ctgcagtcat tcatgggaag ggtcaactat tcctataagg
     3901 acaaatacct gctgaccgcc agtatccggg acgacggttc ttccgtcctg accaaaaaat
     3961 acagcgcttt cccttccgtg gccctgggct ggaatatcgc ccgggagcat ttcatggata
     4021 actc
//
LOCUS       DATGFG010000009         3863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_456580, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000009 DATGFG010000000
VERSION     DATGFG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456580"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..585)
                     /gene="tssD"
                     /locus_tag="VKQ52_00130"
     CDS             complement(190..585)
                     /gene="tssD"
                     /locus_tag="VKQ52_00130"
                     /inference="COORDINATES: protein motif:HMM:NF037603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:1901998 - toxin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HLZ85603.1"
                     /translation="MSFVAKLRLDGEEINVLHCGFRFTQATDTTGKPTARPQGGNINL
                     LVESTGSTDLFDWMIKPTQQKSGAITFYRRDTMSKLKTLEFTDAVCIEYYETFDHTGD
                     TPMQIHLTISAHEIKLNDSEFSNNWPKLK"
     gene            complement(685..1077)
                     /gene="tssD"
                     /locus_tag="VKQ52_00135"
     CDS             complement(685..1077)
                     /gene="tssD"
                     /locus_tag="VKQ52_00135"
                     /inference="COORDINATES: protein motif:HMM:NF037603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:1901998 - toxin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HLZ85604.1"
                     /translation="MSFKAKLKVGGKELNVLGLHYSMNQETDPTGRPSSITRGGKIDI
                     TIESTGDTTFFEWMCNSFERKDGSIVFFKRDSDATLKELGFKEGYLVTYNENFDSTGS
                     NPVTESFTISAKEISMGTGKHINEWVGA"
     gene            complement(1222..2601)
                     /locus_tag="VKQ52_00140"
     CDS             complement(1222..2601)
                     /locus_tag="VKQ52_00140"
                     /inference="COORDINATES: protein motif:HMM:NF036663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5458 family protein"
                     /protein_id="HLZ85605.1"
                     /translation="MSQEASKTAEWQAKVQEAVKSIDKPAEVLDGGIKKLEKFGGFDL
                     LESAIEGVQQVSPERKARKKIFLEESAKKAERESLKKSLQWWSEILSSTDDLSEMVAA
                     CQERVDTADEVLKKNLGAAVRRTKELERSYRSVALFYKNTESTKVKNVSVINVEPEQL
                     KDLDNTRFIDAIQEELVANYDRLDMRNNYGLVVLPGYLGSNKVVEKWAKIAYENKAML
                     VTDFLDLEQPDDIMEEFEAANLTGGDNYRSNIVMTCNWLVGRGKFAEVGEEDDLYVPP
                     AGALAGKIYNTLMSQVVAGKKHGGMNEVDGVRFDLKKTEIAALEKMGLVPMVKEYGKV
                     MAFSAKTLFNGDNLGLQTYSVVRVFDYVAKVMMDFLNRRAFENFNAITRKDLNGQIVK
                     FLDSITGPNKLIETFSIKRFEQDNLDKTKIYLDIFMKPYFPAKTFVVRLEGQKGDDGT
                     EWSSEYAQD"
     gene            complement(2638..3093)
                     /locus_tag="VKQ52_00145"
     CDS             complement(2638..3093)
                     /locus_tag="VKQ52_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85606.1"
                     /translation="MAPEKYNIGGTVAENEAFEAFADIPQNRVLLAEKLTDNAPVKPV
                     IVDGLTTIEQVFEHFHPEVDMDFETEEGTTRQETLHFTNLGDFGIKGITAQSNFLNDL
                     TLKREQYLKMIKQLKTNKLLKQALADPAAKKGLLDSLHALIRELEEAGK"
     gene            complement(3279..>3863)
                     /locus_tag="VKQ52_00150"
     CDS             complement(3279..>3863)
                     /locus_tag="VKQ52_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLZ85607.1"
                     /translation="IRQKPYSVVLFDEIEKAHASVFDIFLQIMDEGKLHDKLGKEGDF
                     SNAVIIFTSNIGSQHVVDSFNKGLVPASADLMEIMSRHFRPEFLGRLTEIVPFAPMTG
                     EMVRNILRIQVKPLIAALDKQGIKLVLTDPAMDRLAQMGYTPQYGARPLGGVIRGQLR
                     RPLSRKLIAGEIGPGSIITLDAKPGGEISWDITS"
BASE COUNT          792 a          912 c         1089 g         1070 t
ORIGIN      
        1 gacccgataa tatcttttga agagtgaagg atggaggagt cgttgccatc cagggcttcc
       61 accgggcata cgacccggaa ctggtgatgg ctatagatgc tttgggatat gtggaatgaa
      121 gtgaccgggg ccagttcttt accatcgatc gcaatatggg cttggacaaa ctgtggcatg
      181 ggagatggtt tattttagct ttggccagtt gttcgaaaat tccgagtcgt tgagctttat
      241 ctcatgggcg cttatcgtga ggtggatctg catcggcgta tcgccggtgt ggtcgaaggt
      301 ctcataatat tcgatgcaga cggcgtcggt gaattcgagg gtctttagtt tgctcatcgt
      361 gtccctccgg tagaaggtga tcgctccgct cttctgctgg gtgggtttta tcatccagtc
      421 gaagagatcg gttgatcccg tgctttccac cagcagattg atatttcctc cctgcggcct
      481 tgcagttggt tttcctgtgg tatccgttgc ctgggtaaag cggaagccgc aatgcagtac
      541 attgatctct tccccatcca ggcgcaactt ggcgacaaat gacataaaaa atgaattagg
      601 tggtgcgcgg gcaatacgaa taagggggaa cggggacctg cgtcacccgt cccgtttcgg
      661 tactattgct atgtatgagg cggatcaggc gcctacccat tcattgatgt gtttcccggt
      721 gcccatcgag atctccttgg cgctgatggt gaaggattcc gtgacggggt tagaaccggt
      781 gctgtcgaaa ttctcattgt acgtgacgag atatccttcc ttgaagccca gttctttcag
      841 ggtagcgtca gagtcgcgtt tgaagaagac gatgctacca tcttttcttt caaaactgtt
      901 gcacatccat tcgaagaaag tggtatcccc ggtagattcg atggtaatgt cgatcttacc
      961 gccgcgggtg atggaagaag gacggcccgt gggatccgtc tcctggttca tggaataatg
     1021 aagcccaagt acattcagct ctttaccacc aacttttaat ttcgctttaa aagacataaa
     1081 aaaggattta aatgaaaaaa atggtattaa aaatgaaaat gattactacg gtcgggtacg
     1141 ggcaaaaggg ctgcgtggcg gctgcaaggg cagtcacccg gcgtcggaag atccggcatc
     1201 gaagggtagg tgaatttctg tttagtcctg tgcgtattcg gaagaccatt ccgtgccgtc
     1261 atcgcctttc tgtccttcca gtctgacgac aaaggtcttt gccgggaaat acggcttcat
     1321 gaaaatatcg agatagatct ttgtcttgtc aaggttgtcc tgctcgaagc gtttgatcga
     1381 aaacgtctcg atgagtttat tcggtccggt aatactatcc aggaatttta caatttgtcc
     1441 gttgaggtcc tttctcgtga tggcgttgaa attctcgaag gccctcctgt tgaggaagtc
     1501 catcatcact tttgccacat agtcgaagac gcgcaccacg gagtaggttt gcagcccgag
     1561 gttgtcgccg ttgaagaggg tcttcgcgga gaaggccatg acctttccat attcctttac
     1621 catcggcacc aggcccattt tttcgagtgc ggcgatctcg gtctttttga gatcgaagcg
     1681 aacgccgtcg acctcgttca taccgccgtg ttttttaccg gccacgacct gggacatgag
     1741 agtgttatag atcttgccgg cgagggcgcc cgcgggcgga acgtagaggt catcctcttc
     1801 gcctacctcg gcgaatttgc cccggcctac cagccagttg caggtcatga cgatattgct
     1861 gcggtaattg tcgccgccgg tgagattggc ggcttcgaac tcttccatga tgtcatcggg
     1921 ctgttcgaga tcgaggaagt cggtgacgag catggccttg ttctcgtagg cgatcttcgc
     1981 ccatttttcc accaccttgt tggaaccaag atagccgggg aggacgacta ggccatagtt
     2041 attccgcata tcgaggcggt cgtagttggc taccagttct tcctggatgg cgtcgatgaa
     2101 ccgggtgttg tcgaggtctt tcagttgctc gggttcgacg tttatgacgg agacattctt
     2161 gaccttcgtg ctttcggtgt tcttgtagaa gagcgcgacg gagcggtagg accgttcgag
     2221 ctcctttgtc ctgcggacgg cggcgccgag gttctttttg agtacttcgt cggcggtgtc
     2281 cactctttcc tggcaggcgg caaccatctc ggagagatcg tcggtactgg agaggatctc
     2341 gctccaccat tggagggatt ttttcaacga ctcacgttcg gccttcttgg cggattcttc
     2401 gagaaagatc tttttacggg ccttcctttc gggacttacc tgctggaccc cttcgatagc
     2461 ggattcgagc aggtcgaagc cgccgaattt ttccagcttt ttgatgcccc cgtccagtac
     2521 ttcggcgggc ttatcgatac tttttacggc ttcctggact ttggcctgcc attcggcagt
     2581 tttcgatgct tcctgagaca tggtaattag ttaggtgaat gatcagttgt acaaaagtta
     2641 tttaccggct tcctccagtt cacggatcag cgcatgaagc gagtcgagca gtcctttttt
     2701 ggctgcggga tcggccagcg cctgtttgag caacttgttg gtctttaact gtttgatcat
     2761 tttcaggtat tgctcgcgtt tgagcgtcag atcgttgagg aagttgcttt gggcggtaat
     2821 accttttata ccgaagtcgc cgaggttggt aaagtggagt gtttcctggc gggtggtgcc
     2881 ttcttcggtt tcgaaatcca tgtctacttc cgggtggaag tgttcgaaca cctgttcgat
     2941 ggtggtgagg ccgtctacaa tgacgggctt tacgggggca ttatccgtta gtttctcggc
     3001 aagcaagacc ctgttctgcg gtatgtcggc aaaggcttcg aatgcttcgt tctccgccac
     3061 ggtgcctcca atgttgtact tttctggagc catatatggg tttttatttt ttatgacgat
     3121 agtctaacca gacctgttga ggtgacagac cggggcagga atcgacaata ggttaatcgt
     3181 aggagaagaa aagctatgaa agcatgtcag gggatttaaa aatagacact tttatcgatt
     3241 aaaacaatcc ctctcctaca ttttatagtt aaataccatt aactggtaat atcccagctt
     3301 atttctccgc ctggttttgc atccagggtg atgatgcttc ccgggccgat ctcgccggcg
     3361 atgagctttc gggagagtgg ccgtctgagc tggccgcgga taacgccgcc cagcgggcgg
     3421 gcgccgtatt gcggggtgta gcccatttgc gccagcctgt ccatcgccgg atcggtgagg
     3481 accagtttta tcccttgttt gtccagcgct gcgataagcg gttttacctg gatgcgcagg
     3541 atattccgga ccatttcgcc cgtcatgggt gcgaagggta cgatctccgt caggcggccg
     3601 aggaattccg gccggaagtg acgggacatg atctccataa gatcggcgga ggcggggacg
     3661 aggcctttgt taaaggagtc cacgacatgc tggctgccga tattggaggt gaagatgatg
     3721 acggcgttcg agaagtcgcc ctccttaccc agtttatcat gcagtttccc ttcgtccatg
     3781 atctggagga agatgtcgaa caccgatgcg tgcgcttttt ctatctcgtc gaagaggacg
     3841 acggaatagg gtttctgccg gat
//
LOCUS       DATGFG010000010         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1483388, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000010 DATGFG010000000
VERSION     DATGFG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483388"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VKQ52_00155"
     CDS             <1..367
                     /locus_tag="VKQ52_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HLZ85608.1"
                     /translation="YAPVWVIATISLSLGIGTMIGWKRIVLTIGEKIGKEHLNYAQGA
                     SAELVAASTIGLSTWFGLPVSTTHVLSSGIAGAMVASNGTKNLNPSTLKAIAMAWVLT
                     LPVSILLALGLFLVFHLFV"
     gene            393..1214
                     /locus_tag="VKQ52_00160"
     CDS             393..1214
                     /locus_tag="VKQ52_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLZ85609.1"
                     /translation="MNKILVATDFSACASNAMEYAMELARVTGVEVVAIHAIGSFEGI
                     FNNTYNALYIEEYHNSKREALRKWAETFSEREAFRAVPVSSTCEVGSVSGVITKYIDA
                     NPVELLVMGTMGSTGIAGLFGSNASTMVEKTKTPTLIVPLESKFSADPVITLATDFST
                     TLSAGDVTALIELIKAFGSPKVTVVNVVEDAAWKTNAAGEAQVREIMPGTHLEFQYIK
                     EDSPTEGILHFVAASATDILCLVKRHHNVVYRIFNTSTVNKVLGKSIKAILVMHE"
     gene            1286..2446
                     /locus_tag="VKQ52_00165"
     CDS             1286..2446
                     /locus_tag="VKQ52_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85610.1"
                     /translation="MTRVLFPLFLFVTVAGMAQKKGVAGAGMAVRTVALRPEAWKFVD
                     GAVQFDDSAGVARMKIVNGRWFVQLKDMDFGDGTIEFDDLPTDPSFALFCFRWQDSLE
                     NECFYFRTTWATGHPYAMEAVQYTPIVKGVMYWDALPHYQTFSDFSVVKPNHVKMILS
                     GRQMRVWVNHMERPTLEVTRLEGNTTHGSLAFQGRHIISNLVVKPGQVEGISPVEGTD
                     PTVFDPRYLCHWQMTEPDTAGEDRQRANRLGIRGIPGDKAVWRPIDAERRGFVNLSRI
                     FGGTIPFGVPRTMWLKTTIHADAARTVQMRLGFLDDVSVFVNDRLIYVDKNAFGFPAV
                     KVLNGRLSIENATISIPLKAGDNVVMMGVGNNFYSWGIVARLDDLEGVMIER"
     gene            complement(2447..3247)
                     /locus_tag="VKQ52_00170"
     CDS             complement(2447..3247)
                     /locus_tag="VKQ52_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ85611.1"
                     /translation="MDTNYFDLMKTRFTSGRNFFGNFSDSVEQLVINQTLAAELGIKK
                     LETLSTVNFLGKKCAIIGIVEDMNFYGFEDKIGPMVFTTAKHTLTPFILVRINSQKIS
                     TTIRSIESFWTKVEPGFPLNYQFLNQSFQQLFSSYESLIKIFTLLTACALLIAFIGLF
                     GLTALITRQRSKEIAIRKIMGASVTQILKLMNGNFVKLSILANLIAWPAAHYLSHRWL
                     DNFAFRIALPISPFLIAVALSLALTIITVCIQTFKFATGSQVELLHDE"
BASE COUNT          776 a          707 c         1021 g          846 t
ORIGIN      
        1 ttatgcgccg gtgtgggtta tcgccacgat ctcgctttcc ctggggatcg ggacgatgat
       61 cggctggaaa cgtatcgtat tgactatcgg tgagaagatc gggaaagaac atctgaacta
      121 tgcgcagggg gccagtgcgg agctggttgc ggcgagcacg atcgggttga gtacctggtt
      181 cgggttgccg gtgagcacca cccatgttct gtcgagtggg atcgccgggg cgatggttgc
      241 ctccaacggt acgaagaacc taaaccccag cacgttaaag gcgattgcga tggcctgggt
      301 gctgaccctg ccggtgtcga tcctgctggc gctgggatta ttcctggttt tccatttgtt
      361 tgtttaaatc tcaacccgaa taaccatacg atatgaacaa gatacttgtt gccaccgact
      421 tttccgcctg tgcctcgaat gccatggaat acgcaatgga gctggcccgg gtcaccggcg
      481 tcgaagttgt cgccatccat gccatcggtt cctttgaagg catcttcaac aatacctata
      541 acgcgcttta tatcgaagaa taccataaca gtaaacggga ggcccttagg aagtgggcgg
      601 agacgttctc cgagcgggag gctttccggg cggtgccggt gtcgagtacc tgtgaagtag
      661 gttcggtgag cggggtgatc acgaaatata tcgacgccaa tccggtggaa ctgctggtga
      721 tgggaacgat gggatcgacg gggatcgccg ggctttttgg gagcaatgcc agtacgatgg
      781 tggagaagac gaagacccct accctgatcg ttccgttgga aagcaagttc tcggccgatc
      841 cggtgatcac gctggcgacg gatttttcga cgacgctgtc tgccggggat gttacggcgc
      901 ttattgaact gatcaaggcc tttgggtcgc cgaaggtgac cgtggtcaat gttgtcgagg
      961 atgcggcctg gaagaccaat gcggccgggg aagcgcaggt ccgggagata atgccgggga
     1021 cgcacctgga attccaatat ataaaagagg attcgccgac cgaagggata ctccattttg
     1081 tcgccgccag cgcgacggat atcctctgtc ttgtcaagcg gcatcacaat gtggtgtacc
     1141 gcatcttcaa caccagcacg gtgaacaagg tgttgggtaa gagtatcaag gctattctgg
     1201 tgatgcacga gtaaggttga acggatttga accgggtttg aaacctgttt tgccgtgatg
     1261 gcggccatat ttgaggaaaa aagctatgac aagagttctt tttcctcttt ttttatttgt
     1321 gactgtcgcc gggatggcgc agaagaaggg tgttgccggg gctggtatgg ccgtgcggac
     1381 cgtggctctg aggccggagg cctggaagtt tgtggacggg gcggtacagt ttgatgacag
     1441 tgcgggggtg gcgcggatga agatcgtgaa cgggcggtgg tttgtgcagt tgaaagacat
     1501 ggatttcggg gacgggacga tcgagtttga cgatttgccg acggacccgt cttttgcgtt
     1561 gttttgcttc cggtggcagg acagcctgga gaacgaatgc ttttatttcc ggaccacctg
     1621 ggccactggg catccctatg ccatggaggc ggtgcagtat acaccgatcg tcaagggcgt
     1681 gatgtattgg gatgccttac cgcattatca gaccttttcg gacttctcgg tggtgaagcc
     1741 caatcatgtg aagatgatcc tgtcgggcag gcagatgcgg gtgtgggtga atcatatgga
     1801 gcggccgacg ctggaggtta cccggttgga gggcaatacg acgcacggga gcctggcttt
     1861 ccaggggagg catatcattt ccaacctggt agtgaagccg gggcaggtgg agggcatttc
     1921 gccggtggag ggtaccgatc ctacggtttt cgatccgcgg tatctgtgtc attggcagat
     1981 gacggagccg gacacggcgg gggaggacag gcagagggct aaccggctgg ggatacgggg
     2041 gatacccggg gataaggcag tgtggaggcc gatcgatgcc gagcggcggg gttttgtgaa
     2101 tctgagccgg atctttggcg ggacgatccc ttttggcgtg ccgaggacga tgtggctgaa
     2161 gacgacgatc catgcggatg cggcccggac ggtgcagatg cgactcggtt ttttggatga
     2221 tgtttcggta tttgtcaatg acaggctgat ctatgtggat aagaatgctt tcggtttccc
     2281 ggcggtgaag gtgctgaatg gaaggttgtc gatcgagaat gcgacgattt ctatcccgtt
     2341 gaaggcgggt gacaatgtgg tgatgatggg ggtagggaat aatttttatt catgggggat
     2401 cgtggcgagg ctggatgacc tggagggggt gatgattgaa cggtagctac tcatcatgta
     2461 ataattccac ttgactgcct gttgcaaatt tgaaggtctg tatgcaaact gtgataattg
     2521 tcaaggcaag tgataacgca acggcgatca aaaacggact gatcggtaaa gcgatccgaa
     2581 aggcaaaatt gtccagccat cggtgagata ggtaatgtgc agcgggccag gcaattagat
     2641 tggctaatat ggataattta acgaaattcc cgttcatcaa tttcaaaatt tgcgttacgg
     2701 aagcccccat tatctttcga atcgcaattt cctttgaacg ctgccgggta attaaggctg
     2761 taagcccgaa tagaccaatg aacgcgataa gcagcgcaca tgcagtgagt agtgtaaata
     2821 ttttgatcaa actctcatag ctggaaaaca attgctggaa tgattgattt aagaattggt
     2881 aattcaaagg gaagccaggt tcgactttcg tccaaaaact ttcaatagat cgaattgttg
     2941 ttgaaatttt ttgggagttt attcgcacca aaataaaggg cgtcagggtg tgctttgcag
     3001 tggtgaatac cattggcccg attttgtcct caaatccata aaagttcata tcctctacaa
     3061 tccctatgat agcacacttc ttgccaagaa agttcacggt tgacagggtt tccaattttt
     3121 taatccccaa ttctgctgcc aacgtttggt tgattaccag ttgctccaca ctatctgaaa
     3181 aatttccaaa gaaatttctc ccagaggtaa acctggtttt cataaggtca aaatagttcg
     3241 tatccataca tagatactgg gtactatatt tgattccgtg ataattgatt ggaagtattg
     3301 ttctctcgtt agagtttcct atggtagaca cagtggaaac gctttggatt
//
LOCUS       DATGFG010000011         5559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_456087, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000011 DATGFG010000000
VERSION     DATGFG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456087"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VKQ52_00175"
     CDS             complement(<1..1264)
                     /locus_tag="VKQ52_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ85612.1"
                     /translation="MLSKSLTIAFRNLWRNKAISAIHVLGLALGIATCLLILLFIRHE
                     WSYDRYNDKGDRIVRVIFRGRMNGGEIKEANVMPPVAATLRKDFPEVEEATRLRANGI
                     HRISYGTKTFREDAMAFADSNFFRVFTLPLVKGDANTALVQPNTVVITQAVARKYFGN
                     DDPIGKVLTFTDDHAALTVTGVIGGVPANSHFHFDLFASMASLPEAKDPSWMTSNFFT
                     YLLLPAGYDAKKLEAKLPGVVDKYMGPQLQKEMGVSLTQFRASGNDIGFFLQPLTDIH
                     LHSDLTGDLSAPGDIRYVYIFGAVAVFMLLIACFNFMNLSTAGASKRAKEVGIRKVLG
                     SLRGQLIGQFLVESLLLTLTAMGMALVLVVLALPFFSRLAGHDLALGWSALPWLLPVL
                     ILFTLLTGLLAGAYPAFFLSAFRPVAVLK"
     gene            1761..2390
                     /locus_tag="VKQ52_00180"
     CDS             1761..2390
                     /locus_tag="VKQ52_00180"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HLZ85613.1"
                     /translation="MNLVTDIQYFPSIIFYKISYNFTHITIEQCETWQKMSFRNRCQI
                     TGAEGIIDLSIPLVNGRDQKSFTRDVKIADSLPWQARHWKTIVSCYNRSPWFEFYRDE
                     LEALYRRPFTFLVDWNLYCLEWSFGVLGMPRSIGLTGEFRKVYTPAEGVDMRGKLVPK
                     NREEWGSDTPRYRQVFEERIGFLPGLSILDLLFCEGKNAIRYIRSSTGG"
     gene            complement(2359..4104)
                     /locus_tag="VKQ52_00185"
     CDS             complement(2359..4104)
                     /locus_tag="VKQ52_00185"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HLZ85614.1"
                     /translation="MHLLTQSALLRALGWTLLNSLWQMGLLWLTWLALTRIFSQAASR
                     FRHGLALALLAFGALGSVVTFFSAYLFDDGGFGVSRAIGFGTGNRITLVLPYCSSLYL
                     LILGGLLIRYSHQYLYSRRLTRAGLSKIPAEFRVFVTSISRRMGIRPAVNVSLSALVD
                     VPMTMGFWKPVILFPVAMISQLSTQQVEAILVHELAHIRRNDYLLNFGVTVIGLLFFF
                     NPFTRMLIHQLQREREHCCDDEVLQFDYDPHAYVSALLSLARQHRQARVALAATGAGG
                     EQLLLQRARKILQQKRTDRRPGARPFILLFLTAAITVFSLSSPPKAVDRQQPALPQPA
                     AGLIGSKEMVFVTIVNTGAPAPAEPKHAAPRNSASRAGSIGHADRPADDQETAARLFA
                     EVASAGNRPGASSRGQTEANSGDQAGASSSDQADAGTLVLTQPATLTFLAPRAKRDNS
                     LGKTEDEDEDEQGPAADDVPPTENLVFVPNSSFSFKNIDTLRPEDRLTWIEESTEREI
                     RTQVEKLQRELGVQLELLRREEARARSLSNASQKQLKQLLNEQMLLQRDYLKKLDNLH
                     LRLKKATRRLTTVYI"
     gene            complement(4107..4487)
                     /locus_tag="VKQ52_00190"
     CDS             complement(4107..4487)
                     /locus_tag="VKQ52_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HLZ85615.1"
                     /translation="MSTKTIKPTESELEILQVLWEKDTASVREVHEELLKTKDAGYTT
                     TLKLMQIMFEKGLVSRDDSFKTHIYQAAVSKEKTQQHLLGKMINSLFGGSSTELVLQA
                     LGNHKASPAELEEIQQLLNNLKKQ"
     gene            complement(4566..5321)
                     /locus_tag="VKQ52_00195"
     CDS             complement(4566..5321)
                     /locus_tag="VKQ52_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85616.1"
                     /translation="MNPSSPVNSRFTTTVLLVLVIGCSPKMSPPPADNSSKPLSQNAD
                     VLVLDLNQPLPDNCRQIGKIKAFGGDSNCTYSGAVADAKAKARKMGGNVLKITGYRDP
                     GLGALCFQIKANVLYSADVQQLIAADQAKKDSVHRSKFGDHPDYAILYAYRPSGAGAL
                     IGYNLHLGDSVICRMKNSSKYEIRIYKEGQTTLWAKTEAKASIPLNVRFGEEYFVRCT
                     IQMGFFVGDPDLSLIEKGPGEAGYEGINDSKSR"
BASE COUNT         1260 a         1488 c         1576 g         1235 t
ORIGIN      
        1 ctttcagcac cgcgacggga cggaaggcgg aaaggaagaa ggcgggatag gcgccggcca
       61 gcagaccggt gagcagggta aaaaggatca gcaccggcag cagccaggga agagcgctcc
      121 aacccagcgc caggtcatgc ccggccagcc ggctgaagaa gggcagggcc agcactacca
      181 gcaccagggc catacccatc gccgtcaggg ttagcagcag ggactcgacc aggaactggc
      241 cgatcagctg cccgcgcagg gagcccaaca ccttgcgaat gccgacctcc tttgcccttt
      301 ttgacgcccc ggccgtgctc aggttcataa aattgaaaca cgcgatcagc aacatgaaca
      361 ccgccaccgc gccgaagatg tagacgtacc ggatatcccc gggcgccgaa agatcgcccg
      421 tcaggtcgga atgcaggtgg atatcggtca agggctgcag aaagaatccg atatcgttgc
      481 cgcttgccct gaactgggtc aggcttacgc ccatttcttt ttgcaattgg ggacccatgt
      541 atttatcgac caccccgggc agtttggcct cgagcttttt ggcatcgtat cccgcgggga
      601 gcaggagata ggtgaaaaaa ttggaagtca tccagctggg gtcctttgcc tccggcaggc
      661 tggccatgga ggcaaaaaga tcgaaatgga aatgcgagtt cgcgggtacc cccccgatca
      721 cgccggtcac ggtgagggcg gcatggtcat cggtaaacgt caacaccttc ccgatagggt
      781 cgtcattgcc aaaatatttc cgcgccaccg cctgcgtgat gacgacggtg ttgggttgca
      841 ccagggccgt attcgcatcc cctttcacca gcggcagcgt aaagacccgg aagaaattgg
      901 agtcggcaaa agccatcgca tcctcgcgga aggtctttgt accatagctg atgcggtgaa
      961 tcccgttggc acggagccgg gtggcttcct ctacctctgg aaaatccttc cggagggtag
     1021 cggcaacggg tggcatcaca ttcgcctctt ttatctcccc gccgttcatc cggccccgga
     1081 agatcacccg gacgatccgg tcgcccttgt cattgtaccg gtcgtaactc cactcatgac
     1141 ggataaaaag caggatcagt aaacaggtgg caatacccag ggcaagcccc aggacatgga
     1201 tggcggaaat ggctttgtta cgccaaaggt tccggaaggc gatggtcaat gacttgctta
     1261 gcatactagc tggttttgcc aattagacaa cagggttagg ttaaaggttg cagctttttc
     1321 aaaaaaagat ggcaggttct tccaaaaaga gggcagggtt ttcaagaacg atgataggtt
     1381 ttcccccaac tggatttact gtcagaatgg cattcacctc gtctccctgg ggcctcccgc
     1441 cggccccagg gggagggtag ggaggaggcc cgtggccatc ccttttttag ccgccggcga
     1501 tgcggccata gtttcgtatt tttattgagg tgccatttcg ctcaccaaaa accttgggaa
     1561 cccggccgtg gaacggtcga aaacgcccca aaatggcctg ctaatccaga tggtatataa
     1621 ataataacac taaataattg ctttttaatt aaatgtgatc gtttacttac gatttatatt
     1681 tagttatgtt cacccggttt ttgcatccaa taggccaaaa tcagaaaata caacaaaatt
     1741 gttttaatca acttgattta atgaacttag taactgacat acaatatttt ccatcgatta
     1801 tattctataa aatttcgtat aattttacac atatcactat tgagcaatgc gaaacctggc
     1861 aaaaaatgag ttttcgcaat cgctgccaga tcacgggcgc ggaagggatc atcgatctca
     1921 gcattccgct ggtcaatgga agggatcaga agagctttac aagggatgtc aaaattgcag
     1981 atagcttgcc ctggcaggcc cggcattgga agaccatcgt ctcctgttat aaccgttccc
     2041 cctggttcga attctaccgg gatgagctgg aagccctcta ccggagaccg ttcactttcc
     2101 tggtcgactg gaatttgtac tgcctggaat ggtccttcgg ggtgctggga atgccccgga
     2161 gcatcgggct taccggggag ttcaggaagg tctatacccc tgccgaaggg gtcgatatgc
     2221 gggggaaact agtgccaaag aaccgggaag aatgggggtc ggacacgccc cggtatcgcc
     2281 aggtattcga ggaacgcatc ggctttctgc cgggtctctc catcctggac ctgttgttct
     2341 gtgaggggaa aaatgcgatc agatatatac ggtcgtcaac cggcgggtag ctttctttag
     2401 ccgcaggtgc agattatcca gctttttcag gtagtcgcgc tggaggagca tttgttcgtt
     2461 cagcagttgt tttagctgtt tttgcgaggc attggaaagg ctgcgcgccc gggcctcctc
     2521 gcgccgcagc agttccagct ggacgcccag ctcccgctgc aatttctcca cctgggtcct
     2581 gatctcccgc tcggtggatt cttcgatcca ggtgagccgg tcttcaggcc ggagggtatc
     2641 tatattctta aaagagaagc tggaatttgg gacaaagaca aggttttccg ttggcgggac
     2701 atcgtcagcc gcgggcccct gctcgtcctc atcctcgtcc tcggttttgc cgagggagtt
     2761 gtcccgtttg gcgcggggcg cgaggaaggt aagggtggcg ggttgggtca ggacaagggt
     2821 gccggcatca gcctggtcac tggaactggc tcctgcctgg tcgccggaat tggcttcggt
     2881 ctggcctcgc gaactggccc ctggccgatt gccggcgctg gccacctcgg cgaatagccg
     2941 ggcggcggtc tcctggtcgt cggccgggcg atccgcatgt cctatcgatc cggcgcggga
     3001 ggcgctattc cgcggggcgg catgtttcgg ttctgccggg gccggggcgc cggtattcac
     3061 tatcgtgaca aagaccattt ccttcgagcc gatcaggccg gcggccggtt ggggcagggc
     3121 cggctgttgc cggtcgaccg cctttggcgg cgacgacagg ctgaacacgg tgatcgccgc
     3181 cgtcaggaat aacaggataa agggccgggc gccaggccgc cggtccgttc tcttctgctg
     3241 gaggatcttc cttgcgcgct gcagcaacag ttgttcgccg ccggcgccgg tggccgccaa
     3301 ggcgacacga gcctgccggt gctgccgggc cagggagagc agggccgaaa cataggcatg
     3361 cgggtcataa tcgaactgca aaacctcatc gtcgcagcaa tgctcccgct cccgttgcag
     3421 ctgatggatc agcatccggg taaacgggtt gaagaagaac agcagcccga tgaccgtgac
     3481 gccaaaattg agcaggtagt cattccgccg gatatgcgcc agctcatgga ccaggatcgc
     3541 ttccacctgc tgggtggaca gctggctgat catggccacc gggaaaagga tcaccggctt
     3601 ccaaaaaccc atcgtcatcg gaacatccac gagggcggag agactgacat tcaccgcggg
     3661 ccggataccc atccggcggc tgatcgaggt cacaaagacc ctgaactccg ccggtatctt
     3721 cgatagcccc gcccgggtaa gccggcggga atacagatat tgatgtgaat agcggatcag
     3781 caatccgccc agtataagaa ggtagagaga agaacaatag ggtaagacca gggtgatccg
     3841 gttccccgtc ccgaagccga tggcccggga aaccccaaag ccgccgtcgt caaaaagata
     3901 cgcggagaaa aaggtaacca ccgaacccag ggcgccaaag gccagcagcg ccagtgcaag
     3961 cccatgccgg aaacgggatg ccgcctggct gaagatccgg gtcagcgcca gccaggtgag
     4021 ccagagcagc cccatttgcc aaagactgtt caacaacgtc cagcccagcg ccctcagtaa
     4081 agcggattgg gtcaagaggt gcatctctat tgttttttca ggttatttaa cagctgttgt
     4141 atctcctcga gttcggcggg agaagccttg tgattaccca gggcctggag caccagttcc
     4201 gtggaggagc ctccgaagag ggaattgatc atcttgccca acaggtgttg ctgggtcttt
     4261 tccttgctga ccgcggcctg gtagatatgg gtcttgaagg agtcgtcccg gctgaccagt
     4321 cctttttcaa acatgatctg catcagcttc aacgtcgtgg tataaccggc atcctttgtc
     4381 ttcagcaatt cttcgtgcac ttccctgaca ctcgccgtat ccttctccca gaggacctgg
     4441 aggatctcca gctcactttc cgtgggtttg atcgtcttcg tcgacataca atagagattt
     4501 agcaaccaat ttacgacaag tttcgtaaat atgtgttaaa aaattgttta ttgccggaga
     4561 aaagatcagc gggattttga gtcatttata ccctcgtatc ctgcttcgcc cggccctttt
     4621 tcgatgagac tgagatccgg gtcgccaaca aagaacccca tctggatcgt gcatctcaca
     4681 aagtattctt caccgaatct gacatttagg gggatcgatg ccttggcttc cgttttggcc
     4741 caaagggttg tttgaccttc tttataaatc cggatctcgt acttgctgga attcttcatc
     4801 cgacagataa cggaatcgcc gaggtgcagg ttgtacccga tcagcgctcc cgcaccgctc
     4861 ggcctgtagg catataagat ggcatagtca ggatggtcgc cgaactttga ccgatgcacc
     4921 gaatccttct tagcctgatc ggctgcgatc aattgttgca catctgcgga gtataaaaca
     4981 ttcgctttga tctggaagca cagagcacca aggcccgggt ccctgtaacc ggtgattttt
     5041 aggacgtttc cgcccatttt acgggccttg gcctttgcat ccgcaaccgc cccgctgtag
     5101 gtacaatttg aatcgcctcc gaaggccttg atcttgccga tctgtctgca gttatcaggc
     5161 aaaggctgat tcaggtccag aaccagcaca tctgcgtttt ggctcagggg ctttgaagaa
     5221 ttatccgcag gcggcggact catcttcgga gagcatccaa taactaacac gagaagcacg
     5281 gttgttgtga aacgggaatt tacaggggaa gaagggttca tagattgagc aagatttggg
     5341 gggtattgct attgccggtt aaaagtatgc accggcagcc gtatctgaaa ggacctggct
     5401 gctaagcgtt ggttaagaac ctttatagcg cccggtcacg tacgggcaaa aaaaagcccc
     5461 gatgtggaaa caccgggact ttggtatttg gatctgatta gtgtcgtaaa tttaacctta
     5521 ggcctgctct cagcgagcct tgcgggcagt aaagataaa
//
LOCUS       DATGFG010000012         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_114058, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000012 DATGFG010000000
VERSION     DATGFG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114058"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VKQ52_00200"
     CDS             <1..224
                     /locus_tag="VKQ52_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85617.1"
                     /translation="CINIAFDEKGTQDLKEITGNPRYPYIAVVVANRLLAVAESQQQK
                     VRTGTLRIMLDNYSKEEMQEMMDEANKKR"
     gene            273..1946
                     /locus_tag="VKQ52_00205"
     CDS             273..1946
                     /locus_tag="VKQ52_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLZ85618.1"
                     /translation="MANNEFDAIVIGSGISGGMAAKELTEKGLKTIMLERGMDIVHIK
                     DYTEASKEAWDYPHRGERTQEMIKNYPALKRDYPLNERNLSWWASDQDSPYTEVKRFD
                     WFRGYHVGGRSLLWGRQTYRWSDLYFEENAKQGIGVDWPVRYKDIEPWYDHVEPFVGV
                     SGSKEGLPELPDSQFLPAMEMNCVEKDLAQRIKQHFGGKRKIIIGRCAHATVPLNGRQ
                     CQYRDKCWYGCPFGGYFSTQSVTLPAAMATGNLTLRPWSIVTQILYDKDKKRATGVEV
                     LDAQTMKTYTYKAKIVFLNASTLNSAWILMNSATDIWPDGLGSSSGELGHNVMDHHLG
                     VYAGGRVEGYLDKYTYGRRANGIYIPRFRNFFGDKRDYLRGFGYQGGADRGRGVEIAE
                     YSIGTQLKEAMSVPGEWNFGMGGFGETLPYHENKVTLDKTRKDKWGLNVLSMDCELKE
                     NELKMRKDMLDDAKEILEALGVKDINGGDDNPYLGRGIHEMGTARMGHDPKTSVLNKW
                     NQVWDAPNVFVTDGSFMTSAACQNPSLTYMAFTARAADHAVSELKKGNL"
     gene            2022..>3140
                     /locus_tag="VKQ52_00210"
     CDS             2022..>3140
                     /locus_tag="VKQ52_00210"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HLZ85619.1"
                     /translation="MPPVETKPGFANYKPAFPGQTRIAGAKTTTPYKVEKVAGKLGNP
                     FAIVAMPDGRLMVTLKSGTMEIHDKNGALVKKITGLPEVIYAGQGGLLDVAFDPNFTG
                     NKLIYWSYSEKYESGSVTAVAKGKLNEAEGKVDNISVIFRATPATRGNLQFGSRLVFD
                     KHGDLFVSLGEKFAPEVRVQAQQLNSCLGKIIRITTTGKPAAGNPFASQANARPEIIS
                     YGHRNPEGLDIDPATGELWESEFGPHGGDEINLIRPGRNYGWPVITYGLEYSGDKVGD
                     GIQQKAGMEQPVYYWDPVVSPSGICFYNGNAIPEWKGNLFVACLSGQHLDRLIIRNNK
                     VVGEERLLVDKDQRIRDVTYMNNMLFAITDDGDIYRIEK"
BASE COUNT          835 a          845 c          882 g          578 t
ORIGIN      
        1 cctgtataaa cattgctttt gacgaaaagg gaacacagga ccttaaagag ataaccggaa
       61 acccccgata cccatatatt gcggtggtcg tcgcaaatcg actattagcc gtggcggaga
      121 gtcaacaaca aaaagtaagg acgggtaccc ttagaattat gctggataac tatagcaagg
      181 aagagatgca ggaaatgatg gatgaagcaa acaaaaaacg gtaagccaaa aaaaatgcat
      241 tccggatgag aaatttgaaa tagatttatg ctatggcaaa caatgaattc gatgctatcg
      301 tcatcggctc cggcatcagc ggcgggatgg ccgcaaaaga actgaccgaa aaaggtctga
      361 aaacgatcat gctcgaacgc ggcatggaca tcgtacacat caaggattat accgaagcca
      421 gcaaggaagc ctgggattac ccccaccgcg gtgagcgcac ccaggagatg atcaagaact
      481 atcccgccct caaaagggac tacccgctga atgaaagaaa cctgtcctgg tgggctagcg
      541 accaggatag cccctatacc gaggtcaaac gattcgattg gttccggggt taccatgtcg
      601 gcgggcgctc gctcttgtgg ggccgccaga cctatcgctg gagcgacctg tatttcgaag
      661 aaaatgcgaa acagggcatc ggcgtcgact ggcccgtccg ctacaaggat atcgagccct
      721 ggtatgacca tgtcgaaccc tttgtaggcg tcagcggatc gaaggaaggc ctgccggaac
      781 tgcccgatag ccaattcctc ccggcaatgg aaatgaactg cgtggaaaaa gacctggccc
      841 agcggataaa acagcatttc ggcgggaagc ggaagatcat catcggccgc tgcgcgcatg
      901 ccaccgtgcc gctcaacggc agacagtgcc agtatcgcga caagtgctgg tatggctgtc
      961 ccttcggcgg atacttcagc acgcagtccg ttaccctgcc tgccgccatg gcgacgggta
     1021 atcttaccct ccggccctgg tccatcgtca cgcagatcct ctatgacaag gataaaaaac
     1081 gggccacggg cgtagaagtg ctggacgccc agacgatgaa aacctatacc tacaaggcaa
     1141 agatcgtctt tctgaacgcc tccacgctga acagcgcttg gatactgatg aactccgcca
     1201 ccgatatctg gccggatggc ctgggtagca gcagcggaga actgggccac aatgtaatgg
     1261 atcaccacct gggcgtctat gccggcggcc gggtcgaggg atacctggac aaatacacct
     1321 acgggcgaag ggccaacggt atctatattc cccggttccg gaactttttc ggcgataaac
     1381 gcgactatct gcggggtttt ggctaccagg gcggtgcaga ccggggtagg ggagtggaga
     1441 tcgccgaata ttccatcggg acccaactga aggaggcgat gtccgtgccg ggcgaatgga
     1501 atttcggcat gggcgggttt ggcgaaacgc tcccgtacca cgaaaacaaa gttaccctcg
     1561 acaagaccag gaaggacaaa tggggtctca acgtgctctc catggactgt gaattgaagg
     1621 aaaacgaatt gaagatgcgc aaggatatgc ttgatgacgc caaagagatc ctggaggccc
     1681 tgggggtcaa agacatcaac ggcggggatg ataatcccta tctcggccgc ggcatccacg
     1741 agatgggcac ggccaggatg ggtcatgatc caaagacttc ggtgctcaac aaatggaacc
     1801 aggtctggga tgcgccaaac gtatttgtca ccgacggctc ctttatgacc tcggcggcct
     1861 gtcaaaatcc ctccctgacc tatatggcct ttacggcacg cgccgccgac cacgccgtaa
     1921 gcgagttaaa gaaaggaaac ctctagtacc tttaaaaaac tcctcctatg cgggtaaaaa
     1981 ctgctgtttg cctcctggca ataataacat tggggcaaaa tttgcctccc gtcgaaacaa
     2041 aacccggatt cgccaattac aaaccggcat tcccggggca aaccaggatt gccggcgcca
     2101 aaacgaccac accctacaag gtggagaagg tcgccggtaa gctgggcaac cctttcgcca
     2161 tcgtagccat gcccgacggc cgcctgatgg tgaccctcaa atcgggtacg atggagatcc
     2221 atgataagaa tggcgcactc gtcaaaaaga tcaccggact gccggaggtg atctacgcag
     2281 gccagggcgg gctgctggac gtggccttcg atcccaactt caccggcaat aagctcatct
     2341 attggagcta ttcggagaaa tatgagtccg gaagcgtgac ggcggttgca aaagggaaat
     2401 tgaacgaggc cgaaggcaag gtcgacaaca tatcggtgat cttccgggcg acaccggcga
     2461 cacggggtaa cctgcaattt ggctcccggc tggtgtttga caaacacggc gaccttttcg
     2521 tcagcctcgg cgagaaattt gccccggaag tccgggtcca ggcgcagcag ttgaattcct
     2581 gcctgggaaa gatcatcaga ataaccacca caggcaaacc agccgcgggc aatccctttg
     2641 ccagccaggc aaacgcccgg ccggaaataa tctcctatgg ccaccgcaat ccggagggac
     2701 tggatatcga cccggccacc ggtgaactgt gggagagtga gttcgggccg cacggcggcg
     2761 acgaaataaa cctcatccgc cccggcagga actatggttg gccggtgatc acctatggcc
     2821 tcgaatactc cggcgacaaa gtcggcgacg gcatccagca aaaagcaggg atggaacagc
     2881 ccgtgtatta ttgggaccct gtcgtctcgc caagcggcat ctgcttttat aatgggaacg
     2941 ccatccccga atggaagggc aatctattcg ttgcctgcct tagcgggcag catctggacc
     3001 ggcttatcat ccgcaacaac aaggtggttg gcgaagagcg gctgctggta gacaaagacc
     3061 agcgtatccg cgacgtaacc tatatgaaca atatgctctt tgcgatcacg gatgacggag
     3121 atatctaccg gatcgaaaag
//
LOCUS       DATGFG010000013         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_455409, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000013 DATGFG010000000
VERSION     DATGFG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455409"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VKQ52_00215"
     CDS             complement(<1..348)
                     /locus_tag="VKQ52_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85620.1"
                     /translation="MTNKNDKAANYTAEIEVARSGRDVFNAITEQVVKWWGGKDLKGS
                     STKLNDEFVIHHPGAHYSKQKLVEIIPDKKVVWLVTESEMSWLEKDKHEWTNTKMIFE
                     ITVKGGKTLLHFTH"
     gene            complement(485..877)
                     /locus_tag="VKQ52_00220"
     CDS             complement(485..877)
                     /locus_tag="VKQ52_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85621.1"
                     /translation="MTAADYISNQELERQPILSSINAIILQEDKSVSQEVGPMMGKEM
                     ILYKEKGYFKYGLASVKNHISIHIMPIYGGSPLHEKYQKLLPSVRFQKGCLNFKNAAD
                     VPLQTISHLFADCAKVSIAAMMEKREKK"
     gene            complement(1008..2003)
                     /locus_tag="VKQ52_00225"
     CDS             complement(1008..2003)
                     /locus_tag="VKQ52_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLZ85622.1"
                     /translation="MNQSSQLSPGTATNRPSSLLLVLALLAVYIIWGTTYLANRFGLL
                     GMKPFILSTFRYALVAILLLAFAGIRRLSFPSWKNARVLAISGLLMLVGGSGLVVTGE
                     QYINSGATAVIIATEPLLFLLLDRRNRKTYSAGTLTGIALGFAGIFLFSRFTSTGGDA
                     LTGASSGLDVLKGTLLVLVSTVFWVAGTLYSRNQTASTASPVTNAAVQHIAGAIACVL
                     IAALRGDWKGFHPAQVTAAAWGGLAYLVVMGSLVAFMAFMWLVKVQPPAVVSTHTYVN
                     PIVAVLAGWMLANETIGLLQLAALVMVLLGVLLVQSRVRLKFFLGRRQPRCEPGS"
     gene            2170..2730
                     /locus_tag="VKQ52_00230"
     CDS             2170..2730
                     /locus_tag="VKQ52_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLZ85623.1"
                     /translation="MKKVIFAIHTTLDGVVDHMKIGPPDQETGEYFIRLTRDADTFLY
                     GRKIYQLMVPYWPDVAKNTSGQTTARTEFAQAFNAVDKIVVFSRSLDSAEGEKTRIVH
                     TGLHDEVLKLKQEQGKTIMTGGVTLASQLAELGLIDEYHFMVHPIIAGEGRRLFEGIN
                     LQEKLRLRLVESKTFTSGCVLLHYVK"
     gene            2883..3854
                     /locus_tag="VKQ52_00235"
     CDS             2883..3854
                     /locus_tag="VKQ52_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLZ85624.1"
                     /translation="MTTIKKIALGKKGPLVSKLGLGCMRMSSVWGGPGNDESESIATI
                     RMALDSGINFVNTGDFYGSGHNELLVGKAIKGRRDDAFISVKFGAIFYGSQWLGLDLR
                     PIAIKNFINYSLVRLGVETIDLYQPCRLDNVVPLEDVIGTVADLIKEGKVRYLGVSEI
                     DAEQLRKAHSIYPVTALEIGYSLADRQIEQKLLPTAKELGIGVVAFANTAEGLLTGEM
                     KAPLAAGDYHHHFTRFQGENLIRNLEKVDLLKRMAKEKGYTPTQLAIAWVNAQGDHIM
                     PLVSMSRRSRLPENIQAMEILLTPEELNTLNMHFSPGAIAGGTYLQR"
     gene            3922..>4164
                     /locus_tag="VKQ52_00240"
     CDS             3922..>4164
                     /locus_tag="VKQ52_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85625.1"
                     /translation="MPGVIFYSFLSTMRKEKVGFFEHNTLVLQVSGHFTLETTGQKIS
                     MRRGEMLLIRKNQLGEITKTPLPDEDYQTIVICLQED"
BASE COUNT         1057 a          952 c         1170 g          985 t
ORIGIN      
        1 atgcgtaaag tgaagcaggg ttttgcctcc tttcacagtt atttcaaaaa tcatttttgt
       61 atttgtccat tcgtgcttgt ctttttccag ccaactcatt tcgctttcag taacaagcca
      121 tacgactttt ttgtccggaa tgatctcaac caatttctgt tttgaataat gtgcgcccgg
      181 atggtgaata acaaattcat catttaattt ggtgctgctt cctttaaggt ctttgccacc
      241 ccaccatttt accacctgct ccgtgatagc gttgaaaaca tcccggccgg atctggcaac
      301 ctctatttcc gctgtgtaat ttgctgcttt gtcatttttg tttgtcattt ttactttttc
      361 agccaaggta gagcggcggc atcgataagt attgtataaa tcggaactcg acgaaacagt
      421 ttaaacctgc tctgggggga taataaaatt cagtgaaaaa tggtcgacct ggtaaatggt
      481 cattttactt tttctcccgt ttttccatca ttgcggcaat actcactttt gcgcagtcgg
      541 caaataggtg agagatggtt tgcaacggta cgtcagcggc attcttgaaa ttaaggcaac
      601 ctttttgaaa tcgcacggaa ggcagaagtt tttgatactt ttcgtgtagc ggagagccac
      661 cataaatcgg cataatgtga atggatatat gattttttac gctggcgagc ccatacttaa
      721 aatacccttt ctctttatat aggatcattt cctttcccat catggggccg acttcctggc
      781 tcacgctctt atcctcttgc agaataattg cgttaatgga cgacaagatc ggttgtcgct
      841 caagctcttg atttgaaata tagtcagccg cagtcatggt ctttgttcct attagcccaa
      901 atttaggcta tcttattgaa aactggtcgg cggacgcgag ggtttacgcg gcgtgggcgg
      961 ggcgcccacc ggcgggtgac gggggattcg ctgcccttgg gggctgccta gctgccgggc
     1021 tcgcagcggg gctggcgacg gccgaggaag aatttgaggc ggacgcggga ctgtaccagc
     1081 aggacgccga ggagcaccat gaccagggcc gcgagttgta gcaggccgat ggtttcattc
     1141 gccagcatcc agccggcgag gacggcgacg atggggttga cataggtgtg ggtgctgacg
     1201 acggcagggg gctgcacttt taccagccac ataaaggcca taaaggcgac gagcgagccc
     1261 atgacgacga ggtaggccag gccaccccaa gctgccgcgg tgacctgcgc ggggtgaaat
     1321 cccttccagt cgccccggag ggctgcgatg agtacgcagg cgatggcgcc ggcgatgtgc
     1381 tgtacggcgg cgttcgtgac gggggaggcg gtgctggcgg tctggttacg ggaatagagc
     1441 gtccccgcga cccagaacac ggtgctgacg aggacgagga gggttccctt cagcacgtcc
     1501 aggccgctgc tggcgccggt gagagcgtcg ccgccggtgg aggtgaagcg ggagaagagg
     1561 aagatgccgg cgaaaccaag ggcgatgccg gtgagggtgc cggcggaata ggttttcctg
     1621 ttgcgtctgt cgagcaacag gaagagcaac ggctcggtag cgatgatgac ggcggtagcg
     1681 ccggaattga tgtattgttc gccggtgacg acgaggccgg agccaccgac cagcatcagc
     1741 agaccgctga tggccaggac gcgggcgttc ttccacgagg ggaaggacag gcgacggatg
     1801 ccggcgaagg cgagcaggag gatggcgacc agggcatagc gaaacgtgga caggataaag
     1861 ggtttcatgc ccaggaggcc gaagcggttg gccaggtaag tagtgcccca gatgatgtag
     1921 acggcaagca gggcgagtac gagcaggaga cttgacggcc ggttggtagc ggtgcccggg
     1981 ctgagctggg atgactggtt catggtgctg gaatttatct tacacaaaac tagcgcgagt
     2041 ttccgttata tttatagtcc agaacctata attgaggtag tccaatcgat gtgtccctcc
     2101 gacatgaaat tcatttcgac gcagctttta tcgttgtaat gcacccaggg ggtataaata
     2161 taatgaaaaa tgaaaaaagt aatttttgca atccacacga ctttagatgg cgttgtggac
     2221 catatgaaaa ttggtccccc cgatcaagaa acgggggagt attttatccg cctcacacga
     2281 gatgccgata cattcctcta cgggcgtaaa atttatcaat tgatggttcc ctattggccc
     2341 gatgtcgcga aaaacacttc cgggcagaca acagcacgaa ccgaatttgc ccaggccttt
     2401 aacgccgtgg acaagattgt tgtattctca cgatcattgg acagcgctga aggagagaaa
     2461 acgaggatag tacacacggg ccttcacgac gaagtactta aattgaaaca agaacaaggt
     2521 aaaactatta tgacaggggg tgtaacgctt gcttcgcagc ttgcagagct tggtctgatc
     2581 gatgaatacc attttatggt tcacccgata attgcaggag aagggagacg gttgtttgaa
     2641 ggtattaacc tgcaggagaa attacgatta agacttgtcg agtcaaagac ttttacatca
     2701 ggatgtgtgc tgcttcatta cgtgaaatag tgcgagcaaa atctgtgcag gagccttgaa
     2761 gcggttaacc cgggcgcata tccggccggt gagccaccca gacaagtaaa ttgagctata
     2821 agagaaatcg gccatggggt ggaacctcga tctttgtgtc cttactccat aaaataacag
     2881 caatgaccac cattaaaaag atcgccttag gcaaaaaggg tccactcgtg tcaaaactcg
     2941 gtttaggctg tatgcgcatg tcctccgttt ggggcggccc ggggaatgac gaaagcgaaa
     3001 gtatagctac catccggatg gcattggaca gcggtattaa tttcgtgaac accggggact
     3061 tttatggcag cggccataat gagctgctgg tgggcaaagc catcaagggg agaagggatg
     3121 atgcctttat cagtgttaag tttggcgcga tcttttacgg cagtcaatgg ctgggactgg
     3181 acctgcgccc catcgccatt aagaacttca tcaactattc tttggtacgt ttgggcgtag
     3241 agaccattga tctttatcag ccatgcagac tggataatgt cgttccgctg gaagatgtga
     3301 taggaaccgt cgccgacctg atcaaagaag ggaaagtgcg ttacctcggg gtatctgaaa
     3361 tagacgcgga gcaacttcgc aaagcccata gcatatatcc tgtaacagca ttggaaatag
     3421 gctattcgtt ggccgatcgg cagatagaac agaaactcct ccccacggca aaggagctgg
     3481 gtatcggagt ggtagccttt gccaatacgg ccgaagggct gctcaccggg gagatgaaag
     3541 caccgcttgc ggcgggtgac taccaccatc acttcacgcg ttttcaggga gaaaatctga
     3601 tcaggaacct ggagaaagtt gacctattaa agaggatggc gaaggaaaag ggatataccc
     3661 cgacgcaatt ggcgatcgcc tgggtaaatg ctcagggcga tcatattatg ccattggtaa
     3721 gcatgagccg aagatcgcgg ctaccggaga atatacaagc catggagatc ctgttgactc
     3781 ctgaagagct gaataccctg aacatgcatt tttcgccggg cgcaatagcc gggggcacat
     3841 acctgcagcg atagataacc aactccctgg ccaaagccct aatttatata cctttgggta
     3901 gtgatgaatc caactgaaat aatgccggga gttatctttt actcttttct ttcgaccatg
     3961 cgaaaggaga aagtaggctt ctttgagcac aacaccctgg tattacaggt gtctggtcat
     4021 tttacgttgg aaactaccgg ccaaaaaatt tcgatgcgcc ggggagagat gttgctgata
     4081 cgaaaaaatc aactgggaga aattaccaag acacctctgc ccgacgagga ttatcaaacc
     4141 attgtcattt gcctgcagga agac
//
LOCUS       DATGFG010000014         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_455310, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000014 DATGFG010000000
VERSION     DATGFG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455310"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VKQ52_00245"
     CDS             <1..632
                     /locus_tag="VKQ52_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HLZ85626.1"
                     /translation="IKEGWKLLFDGKTTQGWHTYLRDTVGSKWQVKDGALIFDPSQPA
                     SGGGDIVTNDVYENFELHLEWRISKGGNSGIIFDVQEDPKYNATYLTGPEMQVLDNKD
                     ADDNKKQTHLAGCLYDMAGDSTVSKPRPVGEWNQVRLIQDKGHLTFWLNGIKTFEGQI
                     GSEQWNTMVAHSKFQNKAFSDFAKVAKGRIALQQHPGSSGWRNIKIRQL"
     gene            complement(647..1153)
                     /locus_tag="VKQ52_00250"
     CDS             complement(647..1153)
                     /locus_tag="VKQ52_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HLZ85627.1"
                     /translation="MKKTAFIFLFAGCLLLPASRSMAQGKTGCISMSEVIDAMPETKK
                     ADSTLAEYKATLEQQFEAYKAEYNEQANFLSSPDTAKYTHIQLDLKRKTLAELLSKLQ
                     GYDQEAGALFSQRRTALLLPIQKKAEDAIQQVSKENGYAFVFEKENLHIYPPSADILP
                     LVKKKLGL"
     gene            complement(1266..2627)
                     /locus_tag="VKQ52_00255"
     CDS             complement(1266..2627)
                     /locus_tag="VKQ52_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85628.1"
                     /translation="MKVGSIIALLLGFYAGPCAGQEAAAPGAGRLADPVVCRTDPSQS
                     YALYIPKKGNVTALPVVFFFDPHGAGSLPLNKYRSLAEAYGFILVGSNNSKNGNDWPT
                     TGQIWDRLYDDVLHRLGIDGHRIYVCGFSGGAKVASYVAIQHPGIRGVIAGGAALPDG
                     VSADNFPFSFTALAGEGDMNLTDIVATSNDLDKTRTRHRLIVFDGKHEWAPLAVMDQA
                     FAGWRLDAMRDGTAPRDNGFIDGYITRSKGRVAADIQAGHLIKAERECRFSANGVDGL
                     SAEANWFKQKGATLSADSRYRQQDQEQQSLFRKEEELKAVYRQQFQQGDNRYWQQTIK
                     DLDAKTAMATPEKGMYQRLLAYLSLAFYSISNQLITARRSDGARHFVELYKLADPTNS
                     EAWYFSGVLDVREGHIPQAESDLVKAVEEGFRDKQRLRNQPEFQKSFTPASFERIEGK
                     MHR"
     gene            2702..>3129
                     /locus_tag="VKQ52_00260"
     CDS             2702..>3129
                     /locus_tag="VKQ52_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85629.1"
                     /translation="MATKSTSAPVTALFLDIGGVMLTNGWDRRAREAAAMKFGLDIDE
                     LNDRHRMTFDTYEAGKLSLDEYLNRTVFCRQRPFTMEQFRAFMFEQSAAYPEMIDLVK
                     ALKTRHGLKIAVVNNEGRELNEHRIKTFRLNEFVDFFISS"
BASE COUNT          691 a          906 c          854 g          678 t
ORIGIN      
        1 aaataaagga aggctggaaa ttattgtttg acggcaaaac gacccagggc tggcacacct
       61 atctccggga tacggtaggc tcgaagtggc aggtgaagga cggcgccctg atcttcgatc
      121 cctctcaacc cgcaagcggc ggcggcgaca tcgtcaccaa cgacgtttac gagaatttcg
      181 agcttcacct ggaatggcgg atctcgaagg gcggcaacag cgggatcatc ttcgacgtac
      241 aggaagaccc caaatacaac gcaacctatc tcaccggacc ggagatgcag gtgctggata
      301 ataaagatgc ggatgacaac aaaaagcaaa cccatctcgc cggctgtctc tatgatatgg
      361 ccggggattc gaccgtctcc aaaccaaggc ccgtcggcga atggaaccag gtgcggctga
      421 tccaggacaa gggtcaccta acgttctggc tcaatggcat aaagaccttc gaaggccaga
      481 tcggcagcga acaatggaac acgatggtgg cccacagcaa attccagaac aaggcattca
      541 gcgattttgc aaaagtcgcc aaaggcagga tcgccttgca gcagcacccg ggctccagtg
      601 gctggcgcaa cataaagatc cgccagctct aacgccggtc tgccggttat aatcccaatt
      661 tctttttcac caacggaagg atatcggccg aaggcggata gatatgcagg ttttcctttt
      721 cgaaaacaaa ggcatagcca ttctcctttg agacctgctg gatcgcgtct tcggcctttt
      781 tctggatggg cagtaacagc gcggttcgtc tttgactgaa aagagcgccc gcttcctggt
      841 catacccctg cagtttgctg agtagttccg caagggtctt ccgtttcaga tcgagttgga
      901 tatgcgtata ttttgccgta tccggcgacg acaggaaatt cgcctgttca ttgtactccg
      961 ccttataggc ctcgaattgc tgctccagcg tcgccttgta ttccgccagg gtgctgtccg
     1021 ccttttttgt ttcgggcatg gcgtcgatga cctcgctcat gctgatacag ccggtcttgc
     1081 cctgcgccat cgaacgggaa gccggcaaca gcaggcagcc ggcaaataag aagataaaag
     1141 cagtcttttt catttggatg tttttctaaa aatacgaaga cattcgtatc ctaatccggc
     1201 gcgaactgct tttaacactt ttttgaattt ttgcgttgcg gtcaggtaat atgaaatacg
     1261 atgccttatc gatgcatttt cccctcgatc ctttcgaacg aggcaggggt aaaggacttc
     1321 tggaactctg gttgattcct cagccgctgc ttgtccctga acccctcttc cacggccttt
     1381 accaggtcgc tctccgcctg ggggatatgc ccctcccgga cgtcgagcac gccggagaaa
     1441 taccaggcct cgctgttggt ggggtcggcc aacttgtata gctccacgaa atgccgcgcg
     1501 ccgtcactgc gtcgggcagt gatgagctga ttgctgatgg aatagaacgc cagggagagg
     1561 taggccagca ggcgctggta catgcctttc tccggggtcg ccatcgcggt cttcgcatcc
     1621 aggtctttta tcgtctgctg ccagtaccgg ttgtctccct gctggaattg ttgccgatac
     1681 accgccttca gttcctcctc cttccggaac agcgattgct gctcctggtc ctgctgacgg
     1741 taccggctat ctgcggaaag ggtggcccct ttttgtttga accagttggc ttcggcgctc
     1801 agcccgtcga caccgttggc cgagaaccgg cattcccgct ccgcctttat gaggtggccc
     1861 gcctggatat cggcggcaac ccggcccttg ctccgggtaa tatatccgtc gataaatccg
     1921 ttgtccctgg gggcggtccc gtcccgcatg gcatccagcc gccagccggc aaaggcctgg
     1981 tccatcacgg cgaggggcgc ccattcgtgc ttcccatcga atacgatcag ccggtgccgc
     2041 gtccgggtct tgtcgagatc attgctggtc gcgacgatgt cggtgaggtt catatccccc
     2101 tcacccgcaa gcgcggtgaa gctgaacggg aaattgtccg cggaaacgcc atcgggcaac
     2161 gccgccccgc cggcaatgac acccctgata cccggatgtt ggatagccac atagctggcc
     2221 accttggcgc cgccggagaa cccgcagaca tagatccgat gaccatcgat ccccagccgg
     2281 tgaagcacat cgtcatagag ccggtcccag atctggcccg tggtcggcca gtcattcccg
     2341 ttcttcgaat tattgctgcc caccaggatg aacccgtagg cctcagccag ggatctgtat
     2401 ttattcaggg gaagcgagcc ggcaccatgc ggatcgaaaa aaaagacgac gggcagcgcc
     2461 gtcacattcc cttttttcgg gatgtataaa gcataggatt gcgaggggtc cgtccggcag
     2521 accaccgggt cggcaagcct ccccgccccg ggtgcggctg cctcctggcc tgcacaggga
     2581 ccggcgtaaa aacccagcag cagtgcgatg atcgatccaa ctttcatagc ctaaaattat
     2641 ggatgggggg cgggattgca aacgttttcc cgttaaattg cagcatctaa aatctacagc
     2701 aatggcaaca aaatcaacat cggcccccgt gacggcgttg ttcctggaca ttggcggcgt
     2761 aatgcttacc aacggatggg acaggcgtgc gcgtgaagcc gcggcaatga aattcggact
     2821 ggacatcgac gagctgaacg atcgccaccg catgaccttc gacacctacg aagccggcaa
     2881 gctaagcctc gatgagtacc tgaatcgaac ggtcttttgc aggcaacgcc ccttcaccat
     2941 ggaacagttc cgggcgttca tgttcgaaca atcggcggct tacccggaaa tgatcgacct
     3001 ggtcaaggcc ctgaagaccc gccacggatt gaagatcgcg gtggtgaaca acgaaggccg
     3061 ggaactgaac gaacaccgca tcaaaacgtt ccgcctcaac gaatttgtgg atttcttcat
     3121 ctcttcctg
//
LOCUS       DATGFG010000015         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1027008, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000015 DATGFG010000000
VERSION     DATGFG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027008"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VKQ52_00265"
     CDS             complement(<1..1021)
                     /locus_tag="VKQ52_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HLZ85630.1"
                     /translation="MREGSVMYLCPMDYFKDLTGLLKTEQAEDRRSYQALAESTTVAQ
                     RREAGLAWFPIAIRDTELGRGDYLTVEVERTSHQDLPHQLRFGAPAALFSNHDAGNDR
                     VEGTISFQGGNRLKLTLRTDELPEWSRNGRLGIDLLFDDNSYTEMFSALRQAAALMEK
                     KDEGRLVRVLAGQQAPVFDALAPVTLPSLNASQQTAVNRILAAEDLAIVHGPPGTGKT
                     TTLVQAIRALAGREKSPVLVVAPSNTAVDLLSERLSDTGLNVLRVGNPAKVSEQLQSL
                     TLDSRMAEHPSMKEVKRLKKKAGEFRDMAHKYKRNFGPAEREQRKALFDEARAILKEV
                     ERTEQF"
     gene            1087..1419
                     /locus_tag="VKQ52_00270"
     CDS             1087..1419
                     /locus_tag="VKQ52_00270"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ85631.1"
                     /translation="MRRDVFQAIADPTRREIIGMIAHRSLNVNAVAGNFDVSRTAIYK
                     HVKILTECGLIVVKQQGRERYYEARLEKLHEISDWVDQYRQFWIARFDALENYLSEIQ
                     PKNKKKGK"
     gene            complement(1482..2168)
                     /locus_tag="VKQ52_00275"
     CDS             complement(1482..2168)
                     /locus_tag="VKQ52_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85632.1"
                     /translation="MKNSILIALFCVGLLPAQAQKIVEKHLPFAANGFVSMNFQISDS
                     IRILTWKKNEVYVHSSIDVNDNQNNDDYTMVFDETGNTISVSGKLETEKIRRSRKDSS
                     GNHHYNGDCCCCCNWDTRIIHEVYIPENADFSVETINGNIIITGNTAEIKAHSISGYI
                     DLAIAPDRKAEVKMHTISGTMYSNIELPSASRGVRHVGGGSVTADLNGGGKRIDLETI
                     SGNIFFRKES"
     gene            complement(2188..2958)
                     /locus_tag="VKQ52_00280"
     CDS             complement(2188..2958)
                     /locus_tag="VKQ52_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HLZ85633.1"
                     /translation="MKKMKYSFLLVASLCIAGITHAQEYKIPVGDTKDAKLILEDFTG
                     DLPIEGYSGSEIVITTGHRFEQNERSKGLKPIYAAGTDNTGIGLSMEKNGNRILLHCL
                     LPITQSADYHLRVPENIALEITRECGKGGNTSVSNIKNEVDFKGCQDITLKTVTGPLV
                     ISTISGSVTVAFTEIAKDKPISISSVSGEVDVTLPAKTPFSLEMGTVSGNMYSDFDFP
                     SNNEMKRVGGGKLRTDFNGGGTAIRLHSVSGNVYLRKS"
     gene            complement(2999..>3324)
                     /locus_tag="VKQ52_00285"
     CDS             complement(2999..>3324)
                     /locus_tag="VKQ52_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85634.1"
                     /translation="VSYAEQMANPDQQVIDALFNSLNHDKNVNVRLAALYSLSRFADR
                     HIVRDSLVSSLSMQTEPIIQVVLINLLAEKRETKAIAPIKEIIINKKTLKEVKDAAQK
                     SLKVL"
BASE COUNT          644 a          887 c          964 g          829 t
ORIGIN      
        1 tgaactgctc ggtgcgttcc acctctttca ggatagcacg cgcttcgtcg aacagggcct
       61 tgcgttgttc gcgttccgcg gggccaaaat tccgtttgta tttatgcgcc atgtcccgga
      121 actccccggc cttcttcttt aaccgtttca cctccttcat cgaagggtgt tccgccatcc
      181 tgctgtccag ggtcagtgac tgcagttgtt cggacacctt ggcggggttg ccgacccgca
      241 ggacattcag cccggtgtcg gaaagccttt cactcagcag atcgacggcc gtgttgctgg
      301 gtgcgacgac cagcaccggg gacttttcgc gcccggccag tgcacggatg gcctgaacca
      361 gggtcgttgt cttccctgta ccgggtggcc catggacgat ggccaggtct tccgcggcga
      421 ggatgcggtt caccgcggtt tgttgagagg cattcagcga aggcagggtc acgggcgcca
      481 gcgcgtcgaa gacgggtgcc tgctgacccg cgaggacacg taccagccgg ccttcgtctt
      541 tcttttccat gagtgcggcg gcctggcgca gggcgctgaa catttccgta taactgttgt
      601 catcgaacaa caggtcgatg cccagcctgc cgttacgcga ccactcgggc agttcgtcgg
      661 tgcgcagggt cagctttagc cggttgccgc cctggaagga gatggtccct tcgacgcggt
      721 cgttgccggc atcatgattg gagaacagcg cggcaggggc gccaaaacgc agctgatgag
      781 gaaggtcctg gtggctggtg cgctccactt ccaccgtgag ataatcgccg cgacccagtt
      841 cggtgtcgcg gatggcgatg ggaaaccagg ccagaccggc ttcgcggcgc tgggctacgg
      901 tcgtcgactc cgccaacgcc tggtacgagc ggcggtcttc cgcctgttcc gttttcagga
      961 ggccggtcaa atccttgaaa taatccatcg ggcaaagata catcacgctc ccttcccgca
     1021 ttcgccggaa aaatttgtta atgccggtcc tatccgttaa tttgcaacca aaaggtttcc
     1081 ttttctatgc gaagagatgt atttcaggca atagccgacc ccacccgcag ggagatcatt
     1141 ggcatgatcg cccatcgatc ccttaacgtc aatgccgtag ccggcaattt cgatgtgagc
     1201 cgtaccgcga tctacaagca tgtgaagatc ctcacggaat gcgggctcat cgtcgtaaaa
     1261 caacagggcc gtgagcgtta ctacgaggcg cggctggaaa agctccatga gatctcggac
     1321 tgggtggacc agtaccgcca gttctggatt gcccgtttcg acgcgctcga aaattacctc
     1381 agcgaaatcc agccgaagaa taaaaaaaag ggtaaataaa aaaaggccgc cccgctgtac
     1441 cggaccggcc ttcaaccatt caaaaaataa gagccgacgg attaactttc tttcctgaag
     1501 aagatattcc cgctgatcgt ttccaggtcg atccgttttc cgccgccgtt gagatcggcc
     1561 gtgaccgagc cgccgccgac atgtctgacc ccgcggctgg cggagggcaa ttcgatattg
     1621 ctgtacatcg taccgctgat ggtatgcatc tttacttcgg ccttgcggtc gggagcgatg
     1681 gccaggtcga tatacccgct aatgctatgg gctttgatct cggccgtatt gcccgtgatg
     1741 atgatattgc cgttgatcgt ctccacggag aaatcggcat tctcggggat atacacttcg
     1801 tggatgatac gtgtgtccca gttgcagcag cagcaacagt cgccattgta gtgatggtta
     1861 cccgaggaat ccttacggct gcggcgtatt ttctccgtct ccagtttgcc ggagacactg
     1921 atcgtattcc ctgtttcatc aaagaccatc gtatagtcat cgttgttctg gttgtcgttg
     1981 acatcgatgg agctatggac atagacctcg ttctttttcc acgtgaggat gcggatcgaa
     2041 tcggagatct ggaagttcat cgagacaaag ccgttcgcgg cgaaggggag atgtttttcg
     2101 acgatcttct gcgcctgtgc ggggagcagc ccgacacaaa acagcgcgat aaggatacta
     2161 tttttcatat tcgttggttt gttgttgtta gcttttacgc aggtagacgt tcccgctgac
     2221 actatgcagc cgtatcgccg tgccgccgcc attgaagtcg gtccggagct tgccgccgcc
     2281 gacacgcttc atttcgttgt tggacgggaa atcgaaatcc gaatacatat tgccggatac
     2341 cgtccccatt tccaggctga aaggggtctt tgcgggcagg gtcacgtcta cctcgccgct
     2401 gacggaggag atcgagatgg gtttgtcttt ggctatctcg gtgaaggcga cggttacact
     2461 accgctgatg gtagagatca cgagcggacc ggttaccgtt ttgagcgtga tgtcctggca
     2521 acccttgaaa tcgacctcgt tcttgatatt cgagacggac gtgttgccgc ctttgccgca
     2581 ttcccgggtg atctcgaggg cgatattctc ggggacccgg aggtgatagt ccgcgctttg
     2641 cgtgatggga agcaggcagt gaagcaagat ccggttgccg ttcttttcca tggagaggcc
     2701 gataccggtg ttgtccgttc cggcggcata gatcggcttg agacccttgc tccgttcgtt
     2761 ctgttcgaag cggtggccgg tggtgatgac gatctcgctg ccgctgtatc cttcgatggg
     2821 cagatccccg gtaaaatctt ccaggatcaa tttggcgtct ttcgtgtccc cgacagggat
     2881 cttgtattcc tgtgcatggg tgattccggc gatacatagg gacgcgacga gcaggaagct
     2941 atacttcatt tttttcatgt tttactattt tattgggtta tgaaagggtt gccgcttctc
     3001 agagcacttt gaggcttttt tgtgcggcgt cttttacttc tttgagggtc ttcttattga
     3061 tgatgatctc ctttatcggt gcgatggcct ttgtctctct tttttccgcc agcaggttga
     3121 taagcaccac ctggatgatg ggttcggtct gcatactcag cgaagagacc agggaatccc
     3181 ggacgatgtg tctgtcggca aaccgggaaa ggctatagag ggcggccagc cgcacgttga
     3241 cattcttgtc gtggttgagg gaattgaaga gggcatcgat cacctgctga tcggggttgg
     3301 ccatctgttc ggcatagctc acgg
//
LOCUS       DATGFG010000016         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1367928, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000016 DATGFG010000000
VERSION     DATGFG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367928"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VKQ52_00290"
     CDS             <1..729
                     /locus_tag="VKQ52_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85635.1"
                     /translation="VFERSTPAGLPVLVDKVWGLVRRISTSAQVQPQEIQFDCDWTEH
                     TKTSYFSFLRQYRQRSGALLSSTIRLHQVKYPDRTGIPPVDHGVLMFYNMGNIDAGAG
                     SSIYDRSIAHRYTPSLRTYPLTLDLALPIFSWALQVRDGKVIQLLDKMNAGSFEKDTN
                     FIRTGPKGFSTRHAGFLKGYYFQENDAVKIESVSSGDLLEIVGEVNRHTNHRIRDLIF
                     FDLDRQNLLQYDKTLFKEILDHTD"
     gene            686..2944
                     /locus_tag="VKQ52_00295"
     CDS             686..2944
                     /locus_tag="VKQ52_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85636.1"
                     /translation="MTKPSLRKYWIILTSLCTVGTTIALACAGDWGPEYGTSNFTPEV
                     FVDSAYSPFFYSSQFYYGIGHDEAHITRWKYDNIGDWGDWLGKGVTSVEELDYLLDTA
                     DTATIDNAIDWLSGRRSTLPATLSAYHLFTNRNNKKVAAFFHYLHLAKKCETFSLTPI
                     LQPWERDSTKPQPHFDATTLNRQLQEGLNTAADAFLQQRYWFQLLRSQFFNGTPPETI
                     RLFDAYSPRFPHNRIWYRSLAYTAGAYYKQKQYSKANYYYSRVFDSCADLRTVAHYSF
                     HPQEEADWRGTLALCRNAGEKATLWQMLGVFYSDPQRAIARIYELDPRSDKLYLLLSR
                     AINASEQRFGKAAGELDDYMPPSPPDSANKSLLTLVTRIATAGNTSQPWAWQLAAGYL
                     NTLESRYAAATDWYKKAGTTVPHERLPQAQLRLLKMLNTLDAAHTIDHTLEQKLLPDI
                     QWLDSSHNDNGIRTNSAYEWLKKTMAAKYLLTGDRIRSECFVSTSAFYKDNHNVEALK
                     TFLSKPNKTPYEQLCTHLSSIQRGDLFEYQAVISCLDDHIDEALAAIKEADSAKATVL
                     PGNPFNARIVDCHDCDHQAAQKIKYSKLAFLQKLKELKDKIAAGSDVYTNAVLMGNAR
                     YNITHFGNARVFYECKVLGSDHSTPFTIDSAFRAPLTSMSTALKYYTLALSAATTDEQ
                     RAKCQYLLARCQRNEWYNTIFAKYDYEFNNGPEFLAWDGFKALKQYPNTNYYKEVLKE
                     CGYFCTYTQKNQ"
     gene            2946..3443
                     /locus_tag="VKQ52_00300"
     CDS             2946..3443
                     /locus_tag="VKQ52_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLZ85637.1"
                     /translation="MLHIGLISDTHHFLDEKVFTHFENCDEIWHAGDFGTIELARRLS
                     DFRPLKGVYGNIDGADIRAVYPEKLRWQSEGVKVFMTHIGGYPPRYNPTVKKELEANP
                     PGLFICGHSHILKVMYDEKLHCLHMNPGAAGRQGWHTVRTLIRFTIDGSNMKDCEVIE
                     LGPRG"
     gene            complement(3516..>3890)
                     /locus_tag="VKQ52_00305"
     CDS             complement(3516..>3890)
                     /locus_tag="VKQ52_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ85638.1"
                     /translation="KSNDQFKNKLILLAEDNVFNANIARRFITGWGAQLEVVVDGRQA
                     LEFVSRRKYDLILMDVQMPELDGFACTRKIRKHFKEVPILAITASPKNEIINEIMACG
                     MNDFVSKPFKPNELRSKLLEYL"
BASE COUNT          947 a         1353 c          851 g          739 t
ORIGIN      
        1 gtcttcgaaa gatcgacacc cgccggctta ccggtgctcg tcgataaagt atgggggctc
       61 gtccggcgta taagcacgtc cgcacaggta caaccacagg agatccagtt cgactgtgac
      121 tggaccgaac atacaaagac cagctatttc tccttcctgc gccaatacag gcagagatcg
      181 ggcgccctcc tttcctccac catccggctt caccaggtga aatatcccga tcgtaccggc
      241 atcccgcccg tcgatcacgg cgtactgatg ttctataaca tgggaaatat cgacgccggc
      301 gccggcagtt ccatctacga ccggtcgatc gcccatcgct ataccccctc cctccgcacc
      361 tatccgctga cactcgatct ggccctgccc atcttctcct gggcgctgca ggtccgcgac
      421 ggcaaggtca tccaattgct cgataagatg aatgccggtt cttttgaaaa agacactaat
      481 tttatccgga cgggaccaaa gggattctcc acccgccacg ccggcttcct caaagggtac
      541 tatttccagg agaacgacgc cgtcaagatc gaatcggtat cttccggcga tctgctggag
      601 atcgtaggcg aagtcaaccg tcacaccaat catcgtatcc gtgatcttat tttctttgac
      661 ctggaccgcc aaaacctcct gcaatatgac aaaaccctct ttaaggaaat actggatcat
      721 actgactagc ctctgcaccg tcggcacgac catcgcccta gcctgcgccg gcgactgggg
      781 tcccgaatac ggaacctcca acttcacccc cgaggtcttc gtggacagcg cctacagtcc
      841 ctttttctac tcctcccaat tctactatgg catcggccac gacgaagccc atatcacccg
      901 ttggaaatat gacaatatcg gcgactgggg tgactggctc ggcaagggcg tcaccagcgt
      961 cgaagaactg gattacctgc tggacaccgc cgataccgcc accatcgaca atgcgatcga
     1021 ctggctcagc ggccgccggt ctaccctccc cgccaccctc tccgcctacc acctcttcac
     1081 caaccgcaac aacaaaaagg tggcggcctt cttccactac ctccatctgg caaaaaaatg
     1141 cgaaaccttt tccctcactc ccatcctcca accctgggag cgggacagca caaaaccaca
     1201 accccatttc gacgcgacaa cactcaaccg gcaactacag gaaggtctca acaccgccgc
     1261 cgacgccttc ctccagcagc gctactggtt ccagctcctc cgaagccaat tcttcaacgg
     1321 cactccaccg gaaactatcc gcctcttcga cgcctacagc ccccggttcc cgcacaacag
     1381 gatctggtat cgcagcctcg cctacaccgc cggcgcatac tacaaacaaa aacaatacag
     1441 caaggccaac tattactata gccgcgtctt cgatagctgc gccgacctcc ggaccgtagc
     1501 ccactacagc ttccatccac aggaagaagc cgactggcgt ggcaccctcg ccctctgccg
     1561 caatgccgga gaaaaagcaa cgctctggca aatgctgggt gtcttctatt ccgatcccca
     1621 aagagccatc gcccgcatct atgaactgga cccccgttcg gataaactct accttctcct
     1681 gtcaagggcg atcaatgcct ccgaacaacg atttggcaaa gccgccgggg aattggatga
     1741 ctacatgccg ccgtcaccgc cggacagcgc caacaagtcc ctgcttaccc tggtcacccg
     1801 catcgccacg gcaggcaata cctcccaacc ctgggcctgg caactggccg cgggctacct
     1861 caacacgctc gaaagccggt atgccgccgc caccgactgg tacaaaaagg ccgggaccac
     1921 cgtcccccac gaacgcctgc cccaggcgca gctccgcctc cttaaaatgc tcaataccct
     1981 cgacgccgcc cacaccatcg accataccct ggagcaaaaa ctgctccccg acatccaatg
     2041 gctcgacagc agtcacaacg acaacggcat ccgcactaac tccgcctacg aatggctgaa
     2101 aaagacaatg gccgccaaat acctcctcac cggcgacagg atcaggtcgg aatgttttgt
     2161 ttccacctcc gccttttaca aggataacca taacgtcgaa gcgctgaaaa ccttcctctc
     2221 caaacccaac aagaccccgt acgaacaact ctgcacccat ctttcctcca tccaacgggg
     2281 agatcttttc gaataccagg ccgtcatcag ctgtctcgac gaccacatcg acgaggcgct
     2341 ggctgcaata aaagaggccg attccgcaaa ggccacggtc ctccccggca accccttcaa
     2401 cgcccggatc gtcgactgcc acgactgcga ccaccaggcc gcacaaaaga tcaaatacag
     2461 caaattagcc ttccttcaaa aactaaagga actgaaagac aagatcgccg ccggctccga
     2521 tgtctatacc aatgcggtcc tcatgggcaa cgcccggtac aacatcaccc acttcggcaa
     2581 cgcccgcgta ttctacgagt gcaaggtgct gggctcggat cacagcacgc cttttacgat
     2641 cgactccgcc ttccgtgccc cgctcaccag catgagcacc gccctgaaat actacactct
     2701 tgcactcagc gccgccacca ccgacgaaca acgcgccaaa tgccagtacc tgctcgccag
     2761 atgccagcgc aatgaatggt acaacacgat tttcgccaaa tacgactacg aattcaacaa
     2821 tggccccgaa ttcctcgcct gggatggttt caaagccctc aaacaatatc caaacaccaa
     2881 ctattacaag gaagtcctga aggaatgcgg ctacttctgt acctatactc aaaaaaatca
     2941 gtaacatgct gcacatcggc ctcatttccg acacccacca tttcctcgat gagaaggtct
     3001 ttacccactt tgagaactgc gatgagatct ggcacgccgg ggacttcggc acaatagagc
     3061 tggcacgccg cctgagtgac ttccgtccgc taaaaggagt ctatgggaat atcgatgggg
     3121 ccgatatccg cgcggtctac ccggaaaaac tgcgctggca atccgaaggt gtgaaggtct
     3181 ttatgaccca catcggtggc tatccacccc gctacaaccc caccgtcaaa aaagaactgg
     3241 aagccaaccc gcccggtctc ttcatctgcg ggcactccca catcctgaaa gtaatgtatg
     3301 atgaaaaact gcactgcctc cacatgaacc ccggagccgc cggccgacag ggctggcaca
     3361 ccgtccggac actcatccgc ttcaccatcg acggcagtaa tatgaaggat tgcgaggtca
     3421 tcgaactcgg cccccgcggc tgagacacac tgggcctgca ccaacctccc ggccctctac
     3481 accacgctgc gcgccccagc ctcggccgtc cgcctttaca ggtactccag caacttgctc
     3541 cgcagctcat tcggtttgaa gggcttgctc acgaaatcgt tcatcccgca ggccatgatc
     3601 tcattgatga tctcattctt cggcgatgcc gttatggcga ggatgggaac ctccttaaaa
     3661 tgtttccgga tcttccgcgt acaggcaaac ccatccagct cgggcatctg cacatccatc
     3721 aggatcaggt catacttccg gcgcgacacg aattccagcg cctgccggcc gtccacgacc
     3781 acctccagct gcgcccccca acccgtgatg aagcgccggg cgatattcgc attgaacaca
     3841 ttgtcctccg ccagcaggat cagtttattc ttaaactggt cattgctctt
//
LOCUS       DATGFG010000017         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1135124, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000017 DATGFG010000000
VERSION     DATGFG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135124"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VKQ52_00310"
     CDS             complement(<1..456)
                     /locus_tag="VKQ52_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85639.1"
                     /translation="MTIAAITENYSEEQLTGFFPGADIRWVILRKVEAVTGTGIGTGA
                     GMSTGVGTGAEVILDLDFTPEKQRCEALSRLQPALVFVNAVTPTLAEMGYPFVRINGW
                     PGFLERSMHELVAPDEAIALRVAELYTRLGRQYRLVPDEPGMISARILST"
     gene            548..928
                     /locus_tag="VKQ52_00315"
     CDS             548..928
                     /locus_tag="VKQ52_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLZ85640.1"
                     /translation="MENKIINTPNAPAPIGPYSQAVKTGNLLFISGQISIDPATGNIE
                     AKDIIAETHQVMHNLRAILTEAGTSFDNVVKTTILLTDMTFFADVNTVYGKYFTGNFP
                     ARETYAVKALPKNVNVEISMIASL"
     gene            complement(929..1459)
                     /locus_tag="VKQ52_00320"
     CDS             complement(929..1459)
                     /locus_tag="VKQ52_00320"
                     /inference="COORDINATES: protein motif:HMM:NF023096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease family protein"
                     /protein_id="HLZ85641.1"
                     /translation="MFIPGFLITLITFPGVIVHELAHQLFCRLYKIPVFKVVYIQMGN
                     PAGYVLHEAVKNKAQGILISVGPFIVNTLLGALISLPAALPVFTFHNAGPLDYLLIYL
                     GVSIAMHAFPSRGDANVIWHSMKESDTPLWVKIIGYPVVGLIYLGSIGSMFWLDLLYG
                     IGVAVFLPKMIISLLG"
     gene            complement(1484..2536)
                     /locus_tag="VKQ52_00325"
     CDS             complement(1484..2536)
                     /locus_tag="VKQ52_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85642.1"
                     /translation="MEENLPVEQPETVSAHDCANCGKPALEGDYPTALCKDCRDHFSR
                     LSVPPWIMVFAAGIGVILVFSLFTFPKNIALGVHLERGKNAMEKGNYYTAEKELRKAL
                     EKFPDNVEANGNLIIASFYNQDYETMAKLVKKLESVNIEDKALYSHISETLEKADRYF
                     PGDSINAFMKDHPRPADIDDSDWANYLGRNPEDCYAMMTYASAVFDKKDYVRCDSILQ
                     RVLKVDDTYFTALLLETSVKRELGDMEASLGYARRILAVNHESAYGMATEARTLLRLK
                     KDQPALELALRANSLRPNVIYIEASLVLAYHFNGRAGDRDALLKSARSQVADSTDREA
                     MQYVQDVMDHKEKFRD"
     gene            complement(2597..>3219)
                     /locus_tag="VKQ52_00330"
     CDS             complement(2597..>3219)
                     /locus_tag="VKQ52_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ85643.1"
                     /translation="DKICDLADRYDAIVMIDDSHSSGFLGKTGRGTHEHCGVMGRVDI
                     ITGTLGKALGGASGGFTSGRKEIIEMLRQRSRPYLFSNSLAPSITGASIAVLDMLSET
                     TALRDKLEYNTTYFRRKMTEAGFDIKPGSHPIVPIMLYDAVVAQNFAARLLEEDIYVI
                     GFFFPVVAKGQARIRVQLSAAHDQHHLDKAIAAFIKVGRELAVLKK"
BASE COUNT          764 a          975 c          794 g          686 t
ORIGIN      
        1 agtcgacaaa atacgtgcac tgatcatccc gggctcatcc ggaacaagcc ggtactgccg
       61 gccaagccgc gtgtagagtt ccgcaacacg caacgcgatc gcttcatcgg gcgccaccaa
      121 ctcatgcatg cttctctcga ggaagcccgg ccagccgttg atccggacaa aaggataacc
      181 catctccgcc agcgtcggcg tcaccgcatt gacaaacacc agcgcgggct gcaaccggct
      241 caacgcctcg caccgctgct tttcaggcgt gaaatccagg tcgagtatta cctccgcacc
      301 cgtacccaca cccgtactca tacccgcacc cgtacctata cccgtacccg tgaccgcttc
      361 taccttccgc aggatcaccc accggatatc cgcgcccgga aaaaatcccg tcaattgctc
      421 ttcactatag ttttcagtga tggctgcaat ggtcatggaa gatgtcgatt tgcaggcaaa
      481 tttagtcatt aaaatcccga caaaacaagc tttggcttct tggccgaaga cgccgatatt
      541 tgtttccatg gaaaacaaga tcatcaatac ccccaacgcc cccgccccca tcgggcccta
      601 tagccaggcc gtcaaaaccg gcaacctcct cttcatctcc ggccagatct ccatcgaccc
      661 ggccaccggc aacatcgaag caaaagacat catcgccgaa acccaccagg tgatgcacaa
      721 cctccgggcc atcctcaccg aagccggcac cagcttcgac aacgtcgtaa agaccaccat
      781 cctgctgacc gatatgacct tcttcgccga cgtgaacacc gtatacggca aatacttcac
      841 cggcaatttc cccgcacgcg agacctacgc ggtaaaagcc ctgccaaaaa atgtgaatgt
      901 ggaaataagc atgatcgcca gcctctgact agcccagcag gctgatgatc atcttgggaa
      961 ggaagaccgc caccccgatg ccatataaca ggtcgagcca gaacatactc ccgatcgaac
     1021 ccaggtagat caaccctacc accgggtaac caatgatctt tacccagagg ggcgtatcgc
     1081 tttccttcat cgaatgccag atcacattgg catcaccccg gctggggaag gcatgcatgg
     1141 caatggagac cccgagatag ataagcaggt agtccagcgg tcccgcgtta tgaaaagtaa
     1201 agacgggcag cgccgccggc agggagatca acgcccccaa tagggtattg acgataaaag
     1261 gaccgacgct gataaggatg ccctgtgcct tgttcttcac ggcctcatgc aacacatacc
     1321 ccgccgggtt acccatctgg atatagacga ccttgaacac ggggatcttg tacagccggc
     1381 agaacagctg atgcgctaat tcatgtacga tgacgccggg aaacgtgatc agcgtaatga
     1441 ggaatccggg aatgaacatg cgggttgttt tttatggttt gggttagtcc ctgaatttct
     1501 ccttgtgatc catcacatcc tggacatact gcatggcctc cctgtccgtg gagtcggcca
     1561 cctgcgaccg ggcgcttttc aacagggcat ccctgtcacc ggcgcggccg ttgaaatgat
     1621 aggcgaggac aagcgatgct tctatataga tcacattcgg ccggaggctg ttggccctga
     1681 gggccagctc cagcgcgggc tggtcttttt tcagccgcaa cagggtcctt gcctcggtag
     1741 ccatgccata ggccgattca tgattgaccg cgaggatcct ccgcgcatag cccagcgaag
     1801 cctccatatc ccccaactct cttttcacgc tcgtctccag taacaaggcg gtaaaatacg
     1861 tatcatccac cttcagcacc cgctgcaaaa tgctgtcaca gcggacatag tcctttttgt
     1921 caaagaccgc cgaggcatag gtcatcatcg cataacaatc ctccgggtta cgccccaggt
     1981 aattcgccca gtcggaatcg tcgatgtcgg ccggccgggg atggtccttc atgaaggcgt
     2041 tgatcgagtc gccgggaaaa taacgatccg ctttttccag ggtctccgag atatggctgt
     2101 aaagcgcctt gtcttcgata ttcaccgatt ccagtttctt caccagcttc gccatcgtct
     2161 cataatcctg gttataaaaa gacgcgatga tcaggttgcc attggcctcc acattgtccg
     2221 gaaacttttc cagcgccttt cgcaattcct tttcggcagt gtagtaattc cccttttcca
     2281 tggcattctt ccccctttcc aggtggacgc caagggcgat attctttggg aaagtaaaca
     2341 gcgagaagac caggatgacg ccgatcccgg cggcaaagac catgatccac gggggaaccg
     2401 aaagccggga aaaatggtcg cggcagtcct tgcacaaagc agtgggatag tcaccttcca
     2461 gcgccggctt gccacagttg gcacagtcat gcgcggatac ggtttcgggt tgttcgacag
     2521 gcaggttctc ctccatgggg ttttgtcaaa aaattgaaga aaaataattt tttgcctcaa
     2581 cccccacaag atggtattat ttcttcagaa ctgcaagctc cctgccgact tttataaagg
     2641 ccgcgatcgc cttgtccagg tgatgctgat cgtgagcagc gcttagctgt acccgaatgc
     2701 gggcctggcc ttttgctacc accgggaaga agaacccgat gacatagatg tcctcttcca
     2761 gcaagcgggc ggcaaagttt tgcgccacca cggcgtcata caacatgatc ggcactatag
     2821 gatgcgaacc cggtttgata tcgaaaccgg cctccgtcat cttccgcctg aagtaggtgg
     2881 tattgtattc gagtttatcc cgcagcgccg ttgtctcgct cagcatatcc agcaccgcga
     2941 tggaagcacc ggtgatggaa ggggccaggc tgttggagaa aagatagggc cggcttcgct
     3001 gccgcaacat ttcgatgatc tctttcctgc cgctggtgaa ccccccgctg gcgccgccca
     3061 gggccttgcc caaggttccc gttataatat cgacgcggcc catgaccccg caatgttcgt
     3121 gtgtcccgcg accggttttc cccagaaaac cggaagagtg ggaatcgtcg atcatgacaa
     3181 tcgcgtcata gcgatcggca agatcacaga tcttgtcca
//
LOCUS       DATGFG010000018         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1250888, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000018 DATGFG010000000
VERSION     DATGFG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250888"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /gene="ruvX"
                     /locus_tag="VKQ52_00335"
     CDS             complement(<1..214)
                     /gene="ruvX"
                     /locus_tag="VKQ52_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HLZ85644.1"
                     /translation="MARIMAIDYGLKRTGIAVTDPLQIIATGLTTVESPKLIPFLKEY
                     MKKETVELILIGEPKNWDDSDTHATPL"
     gene            complement(221..640)
                     /locus_tag="VKQ52_00340"
     CDS             complement(221..640)
                     /locus_tag="VKQ52_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLZ85645.1"
                     /translation="MAFRIFLDAGVLLDLTLKRETYPAARLLMEWAVKGRVQAYVTPS
                     VVQETGRRLREAYGPERAKELLLAILAEVQVIDTGHAVAVSALHSTMSNVDDALSYYT
                     ALHHKLDYFITRSKDLSVAATPVLPVCTPEAFLEHNI"
     gene            complement(630..824)
                     /locus_tag="VKQ52_00345"
     CDS             complement(630..824)
                     /locus_tag="VKQ52_00345"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HLZ85646.1"
                     /translation="MKERLNLTIDGALLDAIKAYAASKEMSVSELVESYFRHVTKPVQ
                     GKNILDLVDELGVPEVKDGV"
     gene            complement(859..1797)
                     /locus_tag="VKQ52_00350"
     CDS             complement(859..1797)
                     /locus_tag="VKQ52_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HLZ85647.1"
                     /translation="MSKVRSYLSLIRFSNTIFAMPFAMIGFALAVFGPKFDAAHLPDF
                     AFANNRVNVYYDGKPAWILFLLVIACMVFARSAAMAFNRWLDVEFDARNPRTAIREIP
                     RGIISKSSALRFVIFNCIAFIVCTWFINPLCFALSFVALAVVLGYSYTKRFTPLCHLV
                     LGVGLSLAPIGAYLAVTGRFAVLPILFSFTVIFWVSGFDIIYALQDEEFDRSQQLYSM
                     PSWLGKAKALRVSEFLHVLSTACVVAAGWYGEFGLWYWAGVAVFMGMLVYQHLLVKPN
                     DLSRVNLAFMTANGIASVVFAGFVIADLFVHNMRMI"
     gene            complement(1794..2342)
                     /locus_tag="VKQ52_00355"
     CDS             complement(1794..2342)
                     /locus_tag="VKQ52_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLZ85648.1"
                     /translation="MRKLGMDELHRKSVNEFRESEKTPVVVVLDNIRSMHNVGSVFRT
                     ADAFLIRAIYLCGYTPQPPHRDIHKTALGATETVEWKYFPSAVAAVNELKTGGYAVFA
                     VEQTEGSVPLPEFSAGLAGPSAVVFGNEVSGVGDEVLAVCDGAIEIPQWGMKHSLNIS
                     IAAGIVLWELVRERVEPNKIEL"
     gene            2379..>3213
                     /locus_tag="VKQ52_00360"
     CDS             2379..>3213
                     /locus_tag="VKQ52_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85649.1"
                     /translation="MRKQTCFRPAALSSVLALGLLLLSSGAMAQDEKIDMATIQKIRE
                     EGLNHSHVMDIAFHLTDASGNRLTASPGFNRAANWAKQQLTDWGLVGATLDPWGEFGK
                     GWELQKSYLALSAPYYKPLIAYPKAWCGGTNSPQSASVLLISATDSAGLERYKGQMAG
                     RILIPEINDTYQQSFKPDAGRYTDEQLDSMSKVVIRPVDTAELRRRRAGFNRGAQAFL
                     NTLRNEALKEGAVAILSESPRGHDGTIFVQGGGSYQPDAPANVLDMVITFEDYSTIVR
                     LL"
BASE COUNT          801 a         1105 c          723 g          584 t
ORIGIN      
        1 ccagcggcgt cgcatgcgtg tcactgtcat cccagttctt tggctccccg atcaggatca
       61 gctccaccgt ctcctttttc atgtattcct tcaaaaaggg gatcagcttc ggcgactcca
      121 ccgtcgtcag gccggtggca atgatctgca acggatcggt cacagcgatc cccgtacgct
      181 tgagcccata atctatcgcc atgatacgcg ccattcctta tcagatattg tgttccagaa
      241 aagcctcggg cgtacacacc ggcaacaccg gcgtcgccgc cacggacagg tccttgctgc
      301 gggtgataaa ataatccagt ttgtgatgca gggccgtata ataagacaag gcatcatcga
      361 cattgctcat cgtcgagtgc agcgcgctca ccgcaaccgc gtgcccggta tcgatcacct
      421 gcacctccgc aaggatcgcc agcagcaact cctttgcacg ctccggtcca tatgcttccc
      481 tcaacctccg ccccgtctcc tgtacgaccg acggcgtcac ataggcctgc acccgcccct
      541 tcaccgccca ctccatcagc agcctggcgg caggataggt ctcgcgcttc aacgtaaggt
      601 caagcaggac cccggcatcc aaaaagatcc taaacgccat ccttcacctc cgggactccg
      661 agttcatcca ccaggtcgag gatattcttc ccctgcaccg gcttcgtcac atgccggaaa
      721 tagctctcca ccagttcgga gacactcatc tccttgcttg cggcataagc ctttatagcg
      781 tccagcaacg caccgtcaat cgtaagattc aaacgctcct tcatagttgc ggtaatggcc
      841 gcaaatatac gcatattatt aaatcatgcg catattatgc acgaaaagat cggcgatcac
      901 aaagccggca aatacaacac tggcaatgcc attggccgtc ataaaagcaa ggttcacccg
      961 gctaaggtca ttgggtttga caagaagatg ttgatacaca agcattccca taaaaacggc
     1021 aacccccgcc caataccata gcccaaactc gccgtaccag cccgcagcca ccacacaagc
     1081 ggtgctcagc acatgcagga attccgaaac ccgcaacgcc ttcgctttcc ccagccagga
     1141 cggcatggaa tacagctgct ggctccggtc gaactcctca tcctgcaacg catagatgat
     1201 atcgaagccg ctgacccaaa agatcaccgt aaaagagaac aggatcggca ataccgcgaa
     1261 tcgtcccgtc accgccaggt aagcgccgat cggcgccagc gaaagcccca cccccaggac
     1321 aaggtggcat aaaggggtaa aacgcttcgt atagctatac cccagcacca ccgccagcgc
     1381 cacaaaggac agcgcaaaac agaggggatt gataaaccag gtacaaacga taaaggcaat
     1441 acaattaaaa atgacaaacc gcaacgcact gctcttcgaa atgatccccc gcggtatctc
     1501 acggatagcc gtacgcggat tccgcgcgtc gaactccaca tccagccagc ggttgaaggc
     1561 catggccgca ctccgggcaa agaccatgca ggcaatgacc agcaggaaca ggatccatgc
     1621 cggttttcca tcatagtaaa cattcacccg gttatttgca aaagcaaaat ccggcagatg
     1681 cgccgcatca aactttggcc cgaatacggc cagcgcaaac ccgatcatcg caaacggcat
     1741 cgcaaaaatg gtgttcgaaa accgaatcaa actcaaataa ctcctgactt tgctcataat
     1801 tctattttat tgggttccac ccgctcccgc accaactccc acaaaacgat ccccgcggct
     1861 atggaaatat tcaacgaatg cttcatcccc cattgcggga tctcgatggc cccgtcacac
     1921 accgccagga cctcatcccc caccccgctc acttcattac caaacacaac ggccgacggc
     1981 cccgccaaac ccgccgaaaa ctccggcaac ggcacactcc cctccgtctg ctccaccgca
     2041 aacaccgcat atccccccgt cttcaactcg ttaaccgccg ccaccgccga agggaaatac
     2101 ttccactcca ccgtctccgt agcgcccagc gccgtcttgt gaatgtcccg gtgcggcggc
     2161 tggggggtat acccgcaaag atagatcgcc cggatgagaa aggcatcggc cgtccggaag
     2221 acactcccca cattgtgcat gctccggata ttgtccagca cgaccaccac cggcgtcttt
     2281 tccgattccc ggaactcatt caccgatttc cggtgcagct catccatccc cagtttgcgc
     2341 atgcgcaaag gtaactacct ttaaaataaa aatcgctcat gagaaaacaa acctgcttcc
     2401 ggccggcggc cttgtcatcc gttcttgccc tggggctgct cctcctttcg tccggcgcca
     2461 tggcacagga tgagaaaata gacatggcca ccatccagaa gatccgggaa gaaggattga
     2521 accactccca cgtcatggac atcgccttcc acctcaccga cgccagcggc aaccgcctca
     2581 ccgcctcccc cggctttaac cgggccgcca actgggcaaa acagcagctt accgactggg
     2641 gactcgtcgg cgccaccctc gacccctggg gagagttcgg caagggatgg gaactgcaaa
     2701 aatcctatct ggccctcagc gcaccctatt ataaacccct catcgcctac ccgaaggcat
     2761 ggtgtggagg aaccaactcc ccgcaatccg cttccgttct cctcatcagt gcaaccgact
     2821 cggccgggct cgaaagatac aaaggccaga tggccggcag gatcctcatc cccgagatca
     2881 acgacaccta ccagcaaagc tttaaaccgg acgccggccg gtacacggac gaacaactgg
     2941 attcgatgtc aaaagtcgtc atacgccccg tcgatacggc cgaactgcgc cgccgccgcg
     3001 ccggcttcaa ccgcggcgcc caggccttcc tcaacaccct ccgcaacgaa gccctgaaag
     3061 aaggcgccgt cgccatcctc agcgagtcac cccgcggcca tgacgggacc atcttcgtcc
     3121 agggcggcgg ctcctatcag cccgatgccc cggcaaacgt cctcgacatg gtcatcacct
     3181 tcgaagacta ttccactatc gtccgcctcc tga
//
LOCUS       DATGFG010000019         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1481306, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000019 DATGFG010000000
VERSION     DATGFG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481306"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VKQ52_00365"
     CDS             <1..448
                     /locus_tag="VKQ52_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLZ85650.1"
                     /translation="RTWPMQHLTCCVGLDDATTENGCLYYVPGSQNWGLLDKPDLAGD
                     MDGLRQYLTEEQKSEFKPVPIQLKKGYATFHHPLMVHGSFSNSSPQARRAFVLNVFTD
                     GTESNTDEVLLKGVPPIKSGHRMEGQFFPLLYSPVQVSPAVVEGEI"
     gene            712..963
                     /locus_tag="VKQ52_00370"
     CDS             712..963
                     /locus_tag="VKQ52_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLZ85651.1"
                     /translation="MTISGLPRHFDKFDLQRLFNPFGWVDYTKIIIDPITGLSKGKGI
                     VEMKQDDHAKQAMAALQGKMLGTNPLNIKEAKDRGPKTW"
     gene            988..2148
                     /locus_tag="VKQ52_00375"
     CDS             988..2148
                     /locus_tag="VKQ52_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HLZ85652.1"
                     /translation="MCTRMVFAFLLVPIALLPAPGNIAWGQKKYTFERPAMGSPFTIT
                     VVADDSLAAARAAAAAFRLTDTLNGILSDYVDSSEINRLSATSGQGRFITVSPPLFDI
                     LRRAQEAARLSKGCYDITIGPVVRLWRKARKTGIPPTREQIAAALLHTGYRYLHLDTA
                     NHSVWLEKPGMQLDVGGLGKGFVAQAALRRIGEMGFSVVMVNAGGKIVTGLAPGTSEK
                     GVNGLGAGAGPGWIIGINAPGEKERLLPTLLSLREMAVATSGDIYQYMEWRGRRYSHI
                     IDPRTGIGLTRSRNVTAIAADGTTADWLATACSILTYRQALRLVRHFPGAALLITEMK
                     QGEIIQKSSINFKKYQLPDAPARPISSAYFFLRPDQPDPVSQERACTVFSAV"
     gene            2120..>2598
                     /locus_tag="VKQ52_00380"
     CDS             2120..>2598
                     /locus_tag="VKQ52_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLZ85653.1"
                     /translation="MHAQSFQPFNQAIPNSGVSFTMVPVPGNKTVDSFWMEEHETTYE
                     EYILFQDESQDHGPMPDGVTRPSPPYIDFTLGMGKKGGFPANSMSRFAAMMYCKWLYR
                     RTGVFYRLPTEAEWEYACRAGAGTSYPFGNDADSLPFYAWFAGNSDNRYHAVKQLKP"
BASE COUNT          586 a          689 c          744 g          579 t
ORIGIN      
        1 cagaacgtgg ccgatgcaac acctcacctg ctgtgttgga ctggacgacg ctaccaccga
       61 aaatggttgc ttatactatg ttcccggaag ccagaattgg ggcctgttgg acaaacccga
      121 cctggcaggg gatatggatg gcttacgaca atacctgacg gaggagcaaa agtcggaatt
      181 caagcctgtt cctatccaac tgaagaaggg ctacgcgacg tttcaccatc ccctgatggt
      241 tcatggctcc ttttccaact cgtcgcccca ggcaaggcgg gcttttgtgt tgaatgtgtt
      301 taccgatggc accgaatcca atacggacga agtgctgctt aaaggggtgc ccccaataaa
      361 atccggacat cggatggagg ggcagttctt tccgttatta tactccccgg tgcaggtgag
      421 ccctgcggtc gtcgaaggag aaatataaga ccgggatcat tcaaaaatat atttctcttt
      481 tattgctgca cctgagttct gcagtaaagg acgagctggt gctggtgtcg atgaaccgga
      541 agggaatgct gatcaaagat tttttcgcct taagcgtacg agttgggcta cgaagacagg
      601 aacctggagg atgtttagta ttgatataca atgttttgtg atggaaattg ttttgagaag
      661 gcgggagaat aggggtcggt gggaaaagct atatatattt gtcctatgcg aatgacaata
      721 tctggccttc cgcgccattt cgacaaattt gatcttcaac gacttttcaa cccatttggc
      781 tgggtggatt atacaaaaat catcatcgac cccatcaccg gcctgagcaa gggaaaaggg
      841 atcgttgaga tgaagcagga cgatcatgcg aaacaggcca tggctgcctt acagggcaaa
      901 atgttgggta cgaatccgct taatatcaaa gaagccaagg accgtggccc aaagacctgg
      961 taacgcttcc ggtttatttt ttcagggatg tgcactcgaa tggtttttgc ctttttactg
     1021 gtcccgatcg ccttgttgcc ggctcccggg aatattgcct gggggcagaa gaaatatacc
     1081 ttcgagcgac ccgccatggg gtcgcctttt accattaccg ttgtggccga tgactccctg
     1141 gccgcggcgc gagccgcggc agcggctttt cgactgacgg ataccctcaa cgggatactg
     1201 agtgactatg tagacagcag cgagatcaac cgtctcagcg ctaccagcgg ccagggccgg
     1261 ttcatcaccg tgtcgccgcc cttgttcgat atcctccggc gcgcgcagga ggcggcgcgg
     1321 ctgagcaagg ggtgttatga cattaccatt ggcccggtgg taaggctgtg gcgcaaggcg
     1381 cgcaagaccg ggatcccgcc aacccgggag cagatcgcgg ctgccttact gcacaccggc
     1441 taccgttacc tgcacctgga tacggcgaat cattccgtat ggctggagaa acccgggatg
     1501 cagctggatg tcggcggact ggggaagggg tttgtcgccc aggccgccct gcggcggatc
     1561 ggggaaatgg gcttttccgt cgtcatggtc aatgcgggtg gaaagatcgt gaccggcctg
     1621 gcgcctggga cgagtgagaa gggagtgaat gggctggggg ctggggcggg gccaggctgg
     1681 atcatcggga tcaacgcacc cggggagaaa gaacgcctgc tccctacgct gctatccctg
     1741 cgggagatgg ccgtcgccac ctccggcgat atttaccaat acatggaatg gaggggccgc
     1801 cgttattctc atatcatcga tccccggacg ggtatcggcc tcacacgcag ccggaatgtt
     1861 acggccatcg ccgccgacgg cactaccgcc gactggctgg caacggcctg cagtatcctc
     1921 acttaccgcc aggcgctgag actggtgcgg catttcccgg gtgcggcgct acttattacg
     1981 gaaatgaaac agggtgaaat aattcagaaa agttccatta actttaaaaa ataccagcta
     2041 cccgatgcgc ctgccagacc tatctcgtcc gcttattttt tcctccgccc tgatcagcct
     2101 gatcctgtca gccaggagcg tgcatgcaca gtcttttcag ccgtttaacc aggccattcc
     2161 caactccggg gtctctttta ccatggttcc ggtgcccggc aacaagaccg tcgactcctt
     2221 ttggatggaa gagcatgaga ctacctatga agagtatatc ctcttccagg acgaatcaca
     2281 ggatcatggt ccgatgcccg atggggtcac ccgccccagt ccgccctaca tcgatttcac
     2341 ccttggcatg ggcaagaaag gcggttttcc cgccaacagc atgagccgct ttgccgcgat
     2401 gatgtattgc aaatggctgt ataggagaac aggcgtcttc tatcggctgc cgacggaagc
     2461 ggagtgggaa tatgcctgcc gtgccggcgc agggacaagc tatccctttg ggaatgacgc
     2521 tgattcgctg ccgttctatg cctggtttgc cggcaacagc gataaccggt accatgccgt
     2581 caaacaacta aagcccaa
//
LOCUS       DATGFG010000020         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_453740, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000020 DATGFG010000000
VERSION     DATGFG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453740"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VKQ52_00385"
     CDS             complement(<1..980)
                     /locus_tag="VKQ52_00385"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HLZ85654.1"
                     /translation="MQDAYIVAGYRTAVGKSKRGKFRFYRPDDMAIDVIRGLLASVPA
                     LDAKRVDDVIVGNAVPEAEQGLQVGRIIAARAVGIHAPGVTVNRYCASGLETIAIATA
                     KIRSGMADCIIAGGTESMTMVPQAGWKTVPAYSIASEEPDYYLNMGLTAEAVAKEFKV
                     SREDQDQFSFDSHRKAINAIEKGYFKPGILPLTVEEVYVDEKGKRQKRTFTVDTDEGP
                     RADTSLDALAKLKPVFAAGGCITAGNSSQTSDGAAFVVVMGEKMMKELGLKPIARLVA
                     CASAGVHPRIMGIGPIEAVPRALRQAGMKLGSIDLVELNEAFASQALAVIR"
     gene            1068..2171
                     /locus_tag="VKQ52_00390"
     CDS             1068..2171
                     /locus_tag="VKQ52_00390"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HLZ85655.1"
                     /translation="MYKILRTLLFFFPAEGVHHFSMKGLKMLCNWGMMRRLVAWSCRP
                     EGKGAGNAATAGGTLERSVLGLRFSNPIGLGAGFDKNALYLRELEALGFGFVEIGTVT
                     PKPQAGNDQPRLFRLPQDQALINRMGFNNDGVEVIASRLKTWREVNGSGEGIRLIVGG
                     NIGKNKVTPNEEAWKDYALCYRALYPYVDYFVVNVSSPNTPGLRELQEKDALRRILVN
                     LQTVGAELSGAGAPRRPLLLKIAPDLSDGQLDDVVSLALEIGLDGLVATNTTISREGL
                     LTPAHRLEAIGAGGLSGAPLRERATEVVRYLTERSGGHIPVIASGGIFTAAHASEKLS
                     AGASLVQVWTGFIYEGPFITNRICRGLQDTGAF"
     gene            2191..2931
                     /locus_tag="VKQ52_00395"
     CDS             2191..2931
                     /locus_tag="VKQ52_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HLZ85656.1"
                     /translation="MHSLFTLDSLISLLTLCLLEIVLGIDNVIFVSLLIGRLPARRQL
                     DARRIWMIAGILVRSALLLGLGWLVTNGNKVLFSIATWGFGIRHLVMIAGGLFLLFKT
                     VEEIHEKLEGGPEEGAVVREGQGKTFGAIITQLILVDTVFSFDSIITAVGLAQKVAVM
                     ITAVILAMIVMFFFSQTIANFINRRPTLKILALSFLLMVGFSLLFEGLEPIHHSHIDK
                     GYIYFSMVFAFGVELLNTRAGKKNRGEK"
     gene            3131..3742
                     /locus_tag="VKQ52_00400"
     CDS             3131..3742
                     /locus_tag="VKQ52_00400"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HLZ85657.1"
                     /translation="MASTDARIDAYIAKSAEFARPILTHLRELVHKACPAVTETVKWG
                     MPFFEYDGSILANMAAFKQHCSFGFWNASQMKDAGSKLQVKDRGAMGNFDRLTALGDL
                     PADKAMLAYIKEAATLIEKGVKKPVAKKAPKADLPVPPALAAALKKNKKAQAAFEGFS
                     PSHRREYIEWITEARTDATRDKRIATTVEWLMEGKSRMWKYRK"
BASE COUNT          826 a         1150 c         1169 g          826 t
ORIGIN      
        1 cggataacgg ccagggcctg cgaggcaaaa gcctcgttca gttccacgag gtcgatactt
       61 cccaatttca tcccggcctg acgcagggcc ctgggaacgg cctcgatggg accgataccc
      121 atgatgcgcg ggtgaacgcc ggcgctggca caggccacca gccgggcgat gggttttagc
      181 cccagttcct tcatcatctt ctcgcccatg acgacgacaa aggcggcgcc atcgcttgtc
      241 tgggaagagt tgccggccgt gatacagccg ccggcggcaa aaacgggttt caatttggcg
      301 agggcgtcca gcgaagtgtc cgcccgtggg ccttcgtcgg tgtcgacagt aaaggtccgt
      361 ttctggcgtt tccctttttc atcgacatat acctcttcga cggtcagcgg aaggatcccg
      421 ggtttgaaat agcccttttc aatggcgttg atcgccttgc ggtgggagtc aaaggaaaac
      481 tggtcctggt cttcccggct taccttgaat tcctttgcga ccgcttcggc cgtgaggccc
      541 atattgagat aatagtccgg ttcttccgat gcgatggagt aggcgggcac cgttttccag
      601 ccggcctgcg gcaccatggt catgctttcc gttcccccgg cgatgataca atcggccatt
      661 cccgaccgga tcttggcggt agcgatggcg atcgtctcca gcccgctggc gcaatagcgg
      721 ttgacggtga cccccggagc atgaataccc acggcgcggg cagcgatgat ccgtcccacc
      781 tgcaaaccct gctcggcctc gggtacggca ttgcccacga taacatcgtc cacccgcttg
      841 gcatccaggg ccggcacgga agccagcagc cccctgatca catcgatggc catatcgtcc
      901 gggcgataga aacggaattt ccctcttttc gatttgccga ccgccgtgcg gtaaccggcg
      961 acaatataag cgtcctgcat gttctttgcg tttagttgtt tatgcaactg tcattctcaa
     1021 agataagatt ttgcatcgaa aaaggaaagt gaaatatctt cgcgcccatg tacaagatcc
     1081 tgcgcacgct actgttcttt ttccccgcgg agggggtcca tcatttttcc atgaaggggt
     1141 tgaaaatgtt gtgtaactgg gggatgatgc ggcgcctggt ggcctggagc tgccggccgg
     1201 aaggaaaggg ggccgggaat gcggcgaccg cagggggaac cctcgagcgg agtgtcctgg
     1261 ggctgcgctt ctccaatccc atcggcctgg gggccggctt cgataagaac gccctctatc
     1321 tgcgcgagct ggaagccctc ggttttgggt tcgtggaaat cggcacggtg accccaaagc
     1381 cccaggccgg caacgaccag ccgcggctgt tccgtctgcc ccaggatcag gcgttgatca
     1441 accggatggg gtttaataat gatggggtgg aggtgatcgc ctcccggcta aagacctggc
     1501 gggaggtcaa cggttccggc gaaggcatcc gcctgatcgt cgggggcaat atcgggaaga
     1561 ataaggtgac gccgaacgag gaggcgtgga aggattatgc cctttgttac cgggcgctct
     1621 atccctacgt agattatttc gtggtgaatg tcagctctcc gaatacgccg ggtttgcggg
     1681 aattgcagga gaaagacgcg ttgcggcgga tccttgtcaa cctgcagacc gtgggtgcgg
     1741 aattgtcggg ggccggggcg ccccggcggc cgctgctgct gaagatcgca cccgatctca
     1801 gtgatggcca gctggacgat gtcgtttccc tggccctcga gatcgggctc gatggactgg
     1861 tggctaccaa taccacgatc agccgcgagg ggttgctgac cccggctcac cggctggagg
     1921 cgatcggggc gggggggctc agcggcgcgc cgctgcgcga acgggcgacg gaggtagtgc
     1981 gctatctcac cgaacgttcg ggcgggcaca tcccggtgat cgcctcgggc ggcatcttca
     2041 ccgccgccca tgcgagcgag aaattatcgg cgggcgcctc gctggtgcag gtgtggacgg
     2101 gattcattta tgaggggccg tttattacca atcgcatctg ccgggggttg caggacaccg
     2161 gcgcctttta actctcttaa ctaaccttga atgcattcct tatttactct tgattccctg
     2221 atcagccttc ttaccctttg tttactggag atcgtgctgg gcatcgacaa cgtcatcttt
     2281 gtctccctgc tgatcggccg gctgccggcc cggcgacagc tggatgcccg ccgtatctgg
     2341 atgatcgccg gcatcctcgt acgcagcgcc ctgctactgg ggctgggctg gctcgttacc
     2401 aatggaaata aagtattgtt ctccatcgcc acctggggtt ttggtatccg ccacctggtc
     2461 atgatcgccg gcggactctt cctactgttc aagaccgtag aggagatcca tgaaaaactc
     2521 gaggggggcc ctgaagaggg ggccgtcgtc agggaaggac agggaaagac cttcggggcc
     2581 atcatcaccc agttgatcct ggtggatacc gtcttttcct tcgacagtat cattacggcc
     2641 gtgggactgg cgcaaaaggt ggccgtcatg atcacggcgg tgatcctggc catgatcgtc
     2701 atgttctttt tctcgcagac catcgccaat tttatcaacc gccgcccgac gctgaagatc
     2761 ctggcgcttt ccttcctgct gatggtcggc ttcagcctcc tgttcgaagg cctcgaaccc
     2821 atccatcact cgcatatcga caagggatat atctattttt cgatggtctt tgcattcggg
     2881 gtggagctgc tgaacacgcg ggccgggaaa aaaaatcgag gcgaaaaata gcggaaaata
     2941 tcatttttcg cctcgatacc caataatgtc tgtacacttc ggccaaggcc tcgtgtacga
     3001 accggattcc atccggttct ggccccaaca gcaaaagacc ccacacaaaa aaaccggggt
     3061 ccccacagaa aaagacccgg gaaaaaagtc ggggaaataa aatacattcg ggtaataaac
     3121 ccacccgtct atggcttcca ccgacgcacg tatcgacgcc tatatcgcga agtccgccga
     3181 attcgccaga cccatcctga cccatctgcg cgaactggtg cacaaggcct gtcccgccgt
     3241 caccgaaacg gtgaaatggg ggatgccctt cttcgagtac gatggcagca tcctggccaa
     3301 tatggccgcg ttcaaacaac attgcagctt cgggttctgg aatgcctcgc agatgaagga
     3361 tgccggcagc aaattacagg tgaaggacag gggcgccatg ggcaatttcg atcggctcac
     3421 cgccctcggg gacctgcccg ccgacaaggc catgctggcc tatataaagg aagccgccac
     3481 ccttatcgaa aaaggggtga aaaagccggt tgccaaaaaa gcgccaaagg cggatctgcc
     3541 ggtcccgccc gccctcgccg ccgcactgaa gaagaacaaa aaggcccagg ccgccttcga
     3601 gggcttctcc ccctcccacc gccgggaata tatcgagtgg atcacggagg ccaggacgga
     3661 cgccacccgg gacaagcgca tagccaccac cgtggaatgg ctgatggaag gcaagtcgag
     3721 gatgtggaaa taccggaagt agcgctaccg ggtataaggg gtttcccgaa ttaataatta
     3781 atttttccgt ttgcgtaagg ccaggtcggg acaaccccct atttttacaa cgcaatacat
     3841 tagtaagccc agcggacaac agattggtca tgcagtctgt tgccgcctgg cactagatct
     3901 cgggcctgca gccggaatca atccaggcac aacagggtgg ccgccacgat accggccgtc
     3961 tccgtacgca g
//
LOCUS       DATGFG010000021         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_111901, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000021 DATGFG010000000
VERSION     DATGFG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111901"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1299
                     /locus_tag="VKQ52_00405"
     CDS             79..1299
                     /locus_tag="VKQ52_00405"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HLZ85658.1"
                     /translation="MPGFELFGPEEQKEINDVMETGILMRYGFDGPRKGIWKSKELEA
                     AICSTLGCGYAQLTSSGTSALSTALACLGIGAGDEVIMPVFTFVASFESVLSVGAIPV
                     LTDVDETLTLAPDAVRRAITPRTKCVMPVHMCGAMADLDALARICKEHNLILLEDACQ
                     SFGARYKGRALGTVGHAGTFSFDFVKTVTCAEGGAVITNDKDLYIKCDGYTDHGHDHT
                     GVDRGADLHPFIGYNYRISELHAAIGLAQIKKLDRFLAIQKKNHGELKAILSTIPGIG
                     FRNIPDPEGDSHTFLSWFLPTEEITRAVIAELRQQNVLAGNFYWYDNNWHYFRKWDHL
                     RKAATLNNLNEDQRKTLLALNPEGFPASDRILGRCVSTAIGLTWTEEQTKEKGEKIVA
                     AVRKVLAREKAPVA"
     gene            complement(1259..>2439)
                     /locus_tag="VKQ52_00410"
     CDS             complement(1259..>2439)
                     /locus_tag="VKQ52_00410"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLZ85659.1"
                     /translation="FRMPKDTSKSAIFVFISTPPSIKTATGKRYYPGLSGLRAIAALT
                     VLFTHVDRLRRLSGMKPLIDPAFNSFIGGTAVTFFFVLSGFLITLLLLNEKAAEGTIR
                     IRSFLANRALRIWPLYYVTLVAGYVVSIFVLQETDADPVSNGFLLNLLLLPNVSFVLG
                     RLPDILIQLWSIGTEEQFYLVWPFFVKRLSAAGLIRVFIIILLFWVVARMPIQLLRVD
                     ADWFNAFLFRTRIDCMAIGGFAALLLFYQESSTGWWSGVYRVILRPLTGWVSALGFIF
                     LLVLSGRYHVSLYQLYALLFAIGCLRVTGLPVRWLEFAPLRYLGKISYGIYLLHHFAL
                     YFLFKVVLVHFHLPAAVIFFLAVLLTTGLAAFSYTFFESFFLKRKVRLRAPSPGPTLS
                     "
BASE COUNT          642 a          751 c          633 g          413 t
ORIGIN      
        1 aacaatgtcg aaagccggcc ggttccgctg cttttctcta actttggcac gaattagtta
       61 atctaaaaat ccttgtatat gcccggtttc gaattattcg gccctgaaga acaaaaagag
      121 ataaacgatg tcatggagac cggtatcctg atgcgatacg gtttcgacgg cccccgcaag
      181 gggatctgga aatcaaaaga gctcgaagcg gccatctgct ctacccttgg ctgcggctac
      241 gcccagctta cctccagcgg aacctccgcc ctgtccaccg cactcgcctg cctggggatc
      301 ggcgccggcg acgaggtcat catgccggta tttacgttcg tagccagctt cgaatccgtg
      361 cttagcgtgg gcgccatccc cgtccttacc gatgtcgacg aaacgcttac cctggcgccg
      421 gatgccgtcc gccgcgccat caccccccgc acgaaatgcg taatgcccgt gcacatgtgc
      481 ggcgccatgg ccgacctcga cgccctggcc cggatctgca aggaacacaa cctcatcctc
      541 ctcgaagacg cctgccagag ctttggcgcg cgatacaagg gccgcgccct gggaaccgtg
      601 ggccacgccg gaaccttctc ctttgacttc gtaaagaccg tcacctgcgc cgaaggcggc
      661 gccgtcatta ccaacgacaa agacctgtac ataaaatgcg atggctacac cgaccatggc
      721 catgaccaca ccggtgtcga ccgcggcgcc gatctccacc ccttcatcgg ctacaactat
      781 cgcatctccg aactgcacgc cgccatcggg ctggcacaga taaaaaaact cgaccgcttc
      841 ctcgccatcc agaaaaaaaa tcacggcgaa ctaaaggcca tcctctccac gatcccaggg
      901 atcgggttcc ggaacatccc cgaccccgag ggcgacagcc acaccttcct cagctggttc
      961 ctccccacag aagagatcac ccgcgccgtg atcgccgaac tgcggcagca gaatgtcctc
     1021 gcgggcaatt tctactggta cgacaataac tggcactatt tccgcaaatg ggatcatctt
     1081 cgcaaggcag cgaccctgaa taatttgaac gaagaccaac gcaagaccct gctggcctta
     1141 aacccggaag gcttccccgc cagcgaccgc atcctgggcc gttgcgtctc caccgctatc
     1201 ggcctcacct ggacggaaga acagacaaag gaaaaaggcg agaagatcgt cgcggccgtc
     1261 aggaaagtgt tggcccggga gaaggcgccc gtagcctgac cttgcgcttc aggaaaaaac
     1321 tctcgaaaaa agtatagctg aaggcggcca gcccggtcgt cagcaacacc gccagaaaga
     1381 agatcacggc agcaggcaaa tgaaagtgga ccagcactac tttaaagaga aaatacagcg
     1441 caaaatgatg gagcaggtat atcccatagg agatcttccc caggtaacgc agcggcgcga
     1501 attccagcca gcgtacaggc agtcccgtca cccggagaca gccgatggcg aataacaggg
     1561 catacagctg gtaaagactg acatgataac gcccgctcaa taccagcagg aaaataaagc
     1621 caagggccga tacccaaccc gtcaatggcc gcaggatcac ccggtacact ccgctccacc
     1681 agcccgtcga tgactcctgg tagaacaata gcagggccgc gaatcccccg atcgccatac
     1741 agtcgatccg cgtgcggaag agaaaggcat tgaaccaatc ggcatcgaca cgcaacagct
     1801 ggatgggcat cctcgccacc acccagaaga ggaggatgat gataaatacc cggatcagcc
     1861 cggcggccga caaccttttg acaaaaaatg gccagacaag atagaactgt tcttctgtcc
     1921 cgatggacca tagctggatc aggatgtccg gcagcctgcc aaggacgaac gagacattgg
     1981 gtaataaaag gaggtttaac aggaacccgt tactcaccgg atctgcatcg gtctcctgca
     2041 acacaaagat ggagaccaca taacccgcca ccagcgtgac ataataaagc ggccagatgc
     2101 gcagcgcccg gttggcaaga aaggatctta tgcggatggt cccctccgcc gccttttcat
     2161 tcagcagcaa cagggtgatc aggaatccgc tcaacacgaa aaaaaaggtg accgccgtcc
     2221 cgccgataaa agaattgaag gcgggatcga taagcggctt catcccggac agcctgcgca
     2281 ggcgatcgac atgcgtaaaa aggaccgtca gggcggcaat ggccctcagc ccggaaagcc
     2341 ccggatagta gcgcttgccg gtggccgtct ttatggatgg aggagtactg atgaagacaa
     2401 atatagccga ctttgatgta tctttcggca tgcgaaaaa
//
LOCUS       DATGFG010000022         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1596789, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000022 DATGFG010000000
VERSION     DATGFG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596789"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VKQ52_00415"
     CDS             complement(<1..282)
                     /locus_tag="VKQ52_00415"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HLZ85660.1"
                     /translation="MRYCLALDLQSDPALIAEYEQWHREVWPEIKASILDGGILQMEI
                     WRLENRLFMIMETGPEFGFEKKAAMDAANPIVQRWEELMWKYQVAIPGGR"
     gene            complement(279..1553)
                     /gene="fucP"
                     /locus_tag="VKQ52_00420"
     CDS             complement(279..1553)
                     /gene="fucP"
                     /locus_tag="VKQ52_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HLZ85661.1"
                     /translation="MSKNKFLLPFILVCTLFFLWAFLHNINGILIPHLKKVCQLSDTQ
                     SSLIDFAVYFGYFVIAIPAGLFSHRFGYKKGILCGLFLFAAGALLFLPAADSRNFVLF
                     LVALFVAASGATFLETVANPYAAHMGAPETSEQRLNFAQSFNGLGAVVAPYIGSTFIL
                     SGIEHSKSELDAMKAHGQLTAYLQQEASSIKPPYLVMAIVLVLVAIAFIFTKLPDVRE
                     EDGGGHGAGFSPKVLRIPHVRWAVIAEFFYVGAQVGVTSFFVRFSRFTAGIPEKEAGN
                     MLGAAMIGFMAGRFLGTFFMRYIRPARLLCLYATINIGLLLVALLAHGTIAVYAMMAI
                     PFFLSIMFPTIFALGIKDLGEETKMASSFLVMSIIGGAFTPVMMGLISDATGSIQVAY
                     IMPVVCMAFILYFGIAGHKIKPVPKTQTQAFA"
     gene            complement(1706..>2217)
                     /locus_tag="VKQ52_00425"
     CDS             complement(1706..>2217)
                     /locus_tag="VKQ52_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85662.1"
                     /translation="VVIGHSRLGKAAVWAGVSDKRFALVVSNESGEGGAALGKRDYGE
                     TIAIINDKFPWWFGSNYKQYGSDPAAMPTDQFMLLSLIAPRPLYVASAQGDQWSDPKG
                     EFLSAHLADPVYHLYDKKGLETDNMPPPEHPVGENIRYHIRNGKHDLTLYDWQQYMNF
                     ADQQFKPKK"
BASE COUNT          517 a          559 c          669 g          472 t
ORIGIN      
        1 gcggccaccg ggtattgcca cctggtattt ccacatcagc tcttcccagc gttggacgat
       61 ggggttggcg gcgtccatgg cggctttttt ttcgaagccg aattccggac ccgtctccat
      121 gatcatgaag agccggtttt cgagacgcca gatctccatc tgcaagatcc cgccatcgag
      181 gatgcttgct tttatttcgg gccagacctc gcgatgccac tgttcgtatt cggcgatcag
      241 ggccgggtcg gattggaggt cgagggcaag acagtatctc atgcgaaggc ctgagtttgg
      301 gttttgggga cgggttttat cttatgaccg gcgatgccga aatacaggat aaaggccata
      361 cacaccaccg gcatgatata ggctacctgg atgctgccag tcgcatctga gatgaggccc
      421 atcatcaccg gagtaaaggc tccgccgatg atcgacatca cgaggaagga ggaagccatc
      481 tttgtttctt cccccagatc tttgatcccc aaagcgaata tggtgggaaa cataatggac
      541 aggaagaagg gaatggccat catcgcatag accgcgatgg tcccatgcgc cagcaaggcg
      601 accaacaaca gcccgatatt gatagtcgca tacaggcata gcaaccgggc cggccggata
      661 tagcgcatga agaacgtgcc caggaaccgg cctgccataa aaccgatcat ggcggcgccc
      721 agcatatttc cggcctcttt ttccggaatc cctgctgtga agcgtgaaaa gcgaacaaag
      781 aagctggtca cgcccacctg ggcccctaca tagaagaatt ccgcgatcac cgcccaacgc
      841 acatgcggta tccggagcac cttcggcgag aacccggctc cgtgcccgcc gccgtcctct
      901 tccctgacat cgggcagctt tgtgaagata aaggctatgg cgacgaggac cagcacgatc
      961 gccatcacca ggtagggcgg ctttatcgaa ctcgcctcct gctgcagata ggccgtcaat
     1021 tggccatggg ctttcatggc atccagttcg gacttggaat gctcgatacc ggacagaatg
     1081 aaggtactgc cgatgtaggg ggcaaccact gcgcccagcc cgttgaacga ctgggcgaaa
     1141 ttgagccgtt gctccgatgt ctccggtgct cccatatggg cggcatatgg attggctact
     1201 gtttccagga aggttgcccc gctggccgca acaaacagcg ctaccaggaa caggacaaaa
     1261 ttgcgactat ccgccgccgg aaggaagagc aaggcgccgg cggcgaaaag gaagagacca
     1321 cagaggatcc ctttcttata accgaagcgg tgagagaaca gtccggcggg gatggcaatg
     1381 acgaagtagc cgaaatagac ggcgaagtcg atgagggagg actgggtgtc gctgagctgg
     1441 cagaccttct taagatgggg gatcagtatc ccgttgatgt tgtgtaaaaa ggcccataga
     1501 aagaaaagcg tacagaccag gatgaacgga agtaagaatt tgttttttga cattagcaag
     1561 cgtttaccgg aaggtcaaag aaaaggcatt ttgatgggta ataagacggg catgttatca
     1621 gagtattcag cttttttaac attatttggc gaatttaaca gatttcaagg aatattcacg
     1681 agcaggaggc agaaggcgac gggtctcatt ttttgggttt gaactgctgg tcggcgaaat
     1741 tcatatactg ctgccagtcg tagagggtga ggtcgtgttt gccattgcgg atatggtagc
     1801 ggatattctc ccccaccggg tgttcggggg gcggcatgtt atcggtttcg aggccttttt
     1861 tgtcatagag gtgatagacg ggatcggcga gatgggcgct gaggaattcg ccttttggat
     1921 cggaccactg gtcgccctgg gcgctggcca cgtagagtgg gcgcggggcg atgagcgaga
     1981 gcagcataaa ttgatccgtg ggcatggccg cgggatcact accgtattgt ttgtagttgg
     2041 agccgaacca ccacgggaat ttgtcgttga tgatggcgat ggtctcgccg tagtcgcgtt
     2101 tgccgagtgc ggcgccgcct tctcccgact cgttggagac taccagggcg aagcgtttgt
     2161 cgctgacgcc ggcccatacg gcagccttgc ccaggcggga atggccgatg acgacaa
//
LOCUS       DATGFG010000023         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_111646, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000023 DATGFG010000000
VERSION     DATGFG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111646"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1399
                     /locus_tag="VKQ52_00430"
     CDS             98..1399
                     /locus_tag="VKQ52_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HLZ85663.1"
                     /translation="MTKKTIAAPAGVGVAKKGSGRTGRLLVRMDPKADWKDILKGVDG
                     FVLERLRVGVIEASSAEQVRRMAAAKNSPFVVEEEERRLRKMPADVSGGAGSSGSLGG
                     GRSGGGSSGSGSSGSGRRGGGLPFGDSRVATWGVQAVNVLKSGFIGKGVKVAVLDTGF
                     DFTHVDFADRAIHRKSFIGRQAIDKDGHGTHCAGVAVGGRRGRGGARYGVASGARLYI
                     GKILDDAGEGSDGLALAGIEWALEKGCRVISMSFGASPEEGDGYSAIFESVAQTVMAH
                     RCLLIAATGNDSKRGRIAPVNHPANCPSVMAVGALTPAMKVAEFSCGGVGLAAAVGRG
                     LSSEATRGGRHVAGPGLDGGGQVDIAAPGDMILSAKVGGGYIVESGTSMAAPFVAGVA
                     ALLWEQYPGASAWEIWARLTQQAQRLALPASAVGAGLVWVR"
     gene            1426..1632
                     /locus_tag="VKQ52_00435"
     CDS             1426..1632
                     /locus_tag="VKQ52_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85664.1"
                     /translation="MHFIAKVEEGQRPNIRAIAESLKGMGVQVRRVMRMTGTITGDSG
                     SLTLGQVKIKGILSVEQDRRLGTK"
     gene            complement(1732..>2897)
                     /locus_tag="VKQ52_00440"
     CDS             complement(1732..>2897)
                     /locus_tag="VKQ52_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ85665.1"
                     /translation="LKGKLALGGKGSGLRSALVVGQFTISLFLIVGTLVVYRQLHFIQ
                     HRDPGYSRDQVLVIKDIDGIPHPEILKQRVLGLSGVANATLTNFLPTGSSRWHNWGTA
                     VTDTPRIVQTELWIVDHDYIPTMDMRLAAGRNFSREMSTDTSAIVINEAAARMFGIAA
                     QPLNKTIRYAAYLHGPADFTVIGVVKDFNFTSIRTAVMPLVLINRPADVPPGLNIRIN
                     AGHIPDVLASVRTLFAAYAPNKPFVYSFMEDDFDAVYKSEQRMGSVVIVLTALAIFIA
                     CLGLFGLAAFAAEQRAKEIGIRKVLGANIPSIVGLLSWDFARLIGLAILIAMPLAGWS
                     MHSWLGNFAYRTTITPWLFVAAAAIVLFIAALTTLFQSLKAAFVNPVETLRAE"
BASE COUNT          543 a          655 c         1132 g          567 t
ORIGIN      
        1 ggtggagagg cggtggtggg cggggagtag gagcttgggt ggcggcatgg cgatgtggcg
       61 gcaaaaaatt atgtttgtgt aattttgctg catttatatg acgaaaaaaa cgatagcggc
      121 gcctgcgggt gtgggtgtcg ccaaaaaagg atcggggcgg acgggaaggt tgctggtgcg
      181 gatggatccg aaggccgatt ggaaagatat tttgaaaggc gtggatgggt ttgtgttgga
      241 gcggttgcgc gtcggggtta tcgaggcctc gtcggcggag caggtgcggc ggatggcggc
      301 cgcgaagaat agtccgttcg tggtggagga ggaggagcgg cgcctgcgca agatgccggc
      361 ggacgtttcc ggtggggccg ggagttcggg cagtttaggc ggaggaagat cgggtggtgg
      421 gagttcgggc tcgggcagct cgggttccgg gaggcggggc ggcggtttgc cgtttggcga
      481 tagccgggtc gccacctggg gtgtgcaggc ggtgaatgtg ttgaagagcg ggttcatcgg
      541 gaaaggcgta aaggtggccg tgctggatac gggttttgac tttacgcatg tagactttgc
      601 cgacagggcg attcatcgca agtcatttat tggccggcag gccatcgaca aggacggtca
      661 tggcacgcac tgcgccggtg tggcggttgg cgggaggagg gggcggggcg gcgcccgata
      721 tggcgtagct tcgggggcgc ggttgtatat cggaaaaatt cttgatgatg cgggagaggg
      781 gtcggatggg ttggcgctgg cggggatcga gtgggcgttg gagaagggat gccgggtgat
      841 ctcgatgtcc tttggcgcgt cgcccgaaga aggcgatggc tattcggcca ttttcgagag
      901 cgtggcgcag acggtgatgg cgcatcgttg tttattgatc gcggcgacgg gtaatgatag
      961 taagcggggc aggatcgcgc cggtgaacca tccggcgaat tgtccgtcgg tgatggcggt
     1021 gggggcgctt accccggcga tgaaggtggc ggagttttcg tgcgggggtg tggggttggc
     1081 cgcggctgtc ggccggggat tgagtagcga ggcgacgagg ggtgggcggc atgtggcagg
     1141 gccggggctg gatggtggcg ggcaggtgga tatcgcggcg ccgggagata tgatattaag
     1201 tgcgaaggtg ggcggcggat atattgtcga gtcggggact tcgatggcgg cgccgtttgt
     1261 ggcgggagta gccgcgcttt tgtgggagca gtatcccggg gcgagtgcat gggagatctg
     1321 ggcgcgactg acgcagcagg cgcagcggct ggcgttgccg gcttcggcgg tgggggcggg
     1381 gttggtgtgg gtgaggtgag agatttactg taattttatc gggttatgca tttcatcgca
     1441 aaagtggaag aggggcagcg gccgaatatc cgggcgatag ccgaatcgct gaaggggatg
     1501 ggggtccagg tcaggcgcgt gatgcggatg acgggcacca tcacggggga cagcgggtcg
     1561 ctgacgttgg ggcaggtgaa gataaagggc atcctgtcgg tggagcagga ccggaggttg
     1621 gggacgaaat aggccttcgg gggaggcccc tcgggggtgc cttgcctgga tctcgcctgg
     1681 atgccggacc gccggaccga ccgaagatgt tgaggggcgg cagccctttt tttattccgc
     1741 gcgcagcgtc tccaccgggt tgacgaaggc ggccttcagc gattgaaaga gggtggtcag
     1801 ggccgcgatg aacaggacga tggccgcggc ggccacaaag agccagggcg tgatcgtcgt
     1861 acgatacgca aaattcccaa gccagctatg catcgaccac cccgccagcg gcatagcgat
     1921 gagtatggcc agcccgatca accgggcaaa atcccaggac aaaagaccca ctatcgacgg
     1981 tatgttggcg ccaagcacct tccggatgcc gatctccttt gcgcgttgtt ccgcggcaaa
     2041 ggcggcgagc ccaaagagcc cgagacaggc gataaagata gccagggccg taaggacgat
     2101 aacgacgctg cccatgcgct gctcactctt gtagacggca tcgaaatcat cttccatgaa
     2161 ggaatagaca aagggtttat ttggcgcata ggcggcaaag agcgttctca cgctcgccag
     2221 tacgtcgggg atatgaccgg cgttgatgcg gatattcagt cccggcggaa cgtcggccgg
     2281 ccggttgatc aggaccaggg gcataacggc ggtgcggata ctcgtgaaat tgaaatcttt
     2341 tacgacgccg atgacggtga agtcggcggg accatgcagg taagcggcat agcggatcgt
     2401 cttattcagc ggctgcgctg cgatgccgaa catccgggcg gctgcctcat tgataacgat
     2461 ggcgctggtg tcggtggaca tttccctgga gaaattgcga cctgctgcca ggcgcatgtc
     2521 catggtgggg atgtagtcgt ggtcgacgat ccataattct gtttggacga tcctgggtgt
     2581 gtcggtgacc gccgtccccc agttgtgcca gcggctggag cccgtgggca ggaaattggt
     2641 gagggtagca ttggcgacgc cggagaggcc gaggacgcgc tgcttcagga tctccggatg
     2701 ggggatccca tcgatatctt ttatgacgag cacctggtca cgcgagtagc cggggtcgcg
     2761 gtgctggatg aaatgcaatt gccggtagac gaccagggtc cccacgatga gaaagaggga
     2821 gatggtgaat tgcccgacga cgagggcgct gcgcagtccg ctgcccttgc cgccaagcgc
     2881 cagtttccct ttcagca
//
LOCUS       DATGFG010000024         3952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_795048, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000024 DATGFG010000000
VERSION     DATGFG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795048"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VKQ52_00445"
     CDS             <1..1722
                     /locus_tag="VKQ52_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ85666.1"
                     /translation="LPRVADSLCFQQFCYLGNGIFWVGSISGVFVYDGHHNTISRIDE
                     IPAEYSLNIAKLSTGSVLVTFYPADYYYFYFQQKLYKIPTGDHPELREIVSVREDGRG
                     RVWFATNSGLSVASREGIEAFCQGKEKSVYYYKYTSSEGLNQIEFNGGLNPSSAISGD
                     GYLGFNSMAGVFLFHQDSVRPTFPAGGIQLYSLGRSGGAVQVGSHLSLEHDNEGKVLQ
                     ASVPYYADRANLLLQYKMDDAPGAWTDIGADGRIAIPPMSHGGHTLSLRTRTGLGSRD
                     YLTKAVNIHVSELFFETPVFRILAGIVLFAICLFFGILIVRLRKEIRLKNDRLLEKNF
                     LLEEALDELKETITLKEKLIALILHDLKTPLYYQHVLLDQLNAADHFTSDDARRLFGD
                     LKTSSASVVKLTKDFLTWYSSQKDGFTVKRTRFEYEEVVRELFSVYSDIAGRKRITMR
                     CKCGGPEWLYTDKDLLEIILRNLLDNAIKYTTDGSITIALERKEDGCAIVVSDTGRGM
                     TADKIKQLEDYAQQPHHQSSETFGYRFIFSLAEKIGAGIRISSEPRKGTNVTIFIPGL
                     NEARLLA"
     gene            complement(1780..2433)
                     /locus_tag="VKQ52_00450"
     CDS             complement(1780..2433)
                     /locus_tag="VKQ52_00450"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HLZ85667.1"
                     /translation="MNTRSITFQVSPSIGTVSAEYFIPEASKCIVTLAHGAGAGMDHS
                     FMVALASALAEAGIATLRFNFPFTEHRKGRPDTPAVAHETIAAAITHAHGSFPSLPLF
                     AAGKSFGGRMTSQYLAAHARPEVKGIVFYGFPLHPSGKPSIERAEHLKAVKVKMLFLQ
                     GTKDELATWDLIESVCASLPKATLVKIEGANHMFKAGKKDVMALLVSATKEWVQKML"
     gene            2626..3318
                     /locus_tag="VKQ52_00455"
     CDS             2626..3318
                     /locus_tag="VKQ52_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85668.1"
                     /translation="MAGLLPGISGLAAISPGLTFLDIPLRLPLAIDLILVPGLFLLAY
                     MIILLVYASRRDGSTGFAQRLGAVFVGGFIVLFCTAFGFLLTYLMQDHLPLSVRRGMN
                     SFGISADLYLPSARYKTIHLQGNFIALLCFIIGIALCIRKIKAPPVMQKVAPLTREQR
                     MTPYERMMEEKRKTAPAAPKTPKTPKKPSPVQRKPSAAHTHNGHTRNLRTPCYNQPLL
                     SIEPEAVNYMPM"
     gene            complement(3394..>3952)
                     /locus_tag="VKQ52_00460"
     CDS             complement(3394..>3952)
                     /locus_tag="VKQ52_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85669.1"
                     /translation="GSFVTLAAQLLDLTATVADDHTVTLTWDVTGVTSLTAFHVQRST
                     DGIAFSDLGNVDVLTGQDRYTFVDRTAVTRVGFYRIQLDETGNAPLVSQVVAVRRSTD
                     GSSQVSLRSAGAGQRVLYIVSDRATRTSLAVIDASGRVCWTGGATLTPGDNYIPLTLP
                     ALSKGVYYLSLGWEGGLRKTTSFLE"
BASE COUNT          916 a         1167 c         1023 g          846 t
ORIGIN      
        1 ctccctcgcg tagccgacag cctttgcttc cagcaattct gttacctggg gaacggcata
       61 ttctgggtag gttccatttc gggcgtattt gtttacgacg gacaccacaa taccatcagc
      121 aggatcgatg agatccccgc cgaatactcc ctcaacatcg cgaaattgag caccggctct
      181 gtcctggtga ccttctatcc cgcggattat tattatttct attttcagca gaaattatac
      241 aaaataccca ccggggatca ccccgagttg cgggagatcg tctccgtgcg ggaggatgga
      301 cgcggcaggg tctggttcgc caccaattcg gggttgtccg tagcctcccg ggaagggatc
      361 gaagcctttt gccaggggaa agaaaaatcg gtctactact ataaatatac ttcaagcgag
      421 gggctcaacc agatcgagtt caacggcggg ctgaacccct ccagcgccat tagcggggac
      481 gggtatcttg gcttcaactc catggcaggc gtcttcctct tccaccagga ttccgtacgg
      541 ccaacctttc ctgcgggagg aatacagctg tattccctgg gcagatccgg cggtgcggtc
      601 caggttggca gccatctgtc cctggaacac gacaatgagg ggaaagtact ccaggcctcc
      661 gtcccctatt atgccgaccg ggcaaacctc ctgttacaat acaagatgga tgatgcaccc
      721 ggcgcctgga cggacatcgg cgccgacggc cggatcgcga tccctcccat gtcccatggg
      781 ggccacaccc tcagccttag gacgaggacg ggcctcggct cccgcgacta tctgacgaaa
      841 gcggtcaaca tccatgtgag cgaactcttt tttgagaccc ctgtattccg gatcctggcg
      901 ggcattgtac tttttgcgat ctgcctgttc tttggcatcc tcattgtccg gctgagaaaa
      961 gagatccggc tgaagaacga ccggcttctc gagaagaatt tcctcctcga agaggccctc
     1021 gatgagttga aagagaccat caccctcaag gagaaactca tcgcgctgat cctccacgat
     1081 ctgaaaacgc cattgtatta tcagcacgtg ctcctcgacc agctcaatgc ggccgatcat
     1141 ttcacaagcg atgacgccag acggttgttt ggggatctga agacctcgag cgcctccgtt
     1201 gtaaagctta caaaggattt cctcacgtgg tacagcagcc agaaggacgg gttcaccgtc
     1261 aaacggaccc ggttcgaata cgaagaggtc gtccgggaac tcttctccgt ctactccgat
     1321 atcgccggcc ggaaaaggat cacgatgcgt tgcaaatgcg gcggaccgga atggctgtat
     1381 accgacaagg atctcctcga gatcatcctc cggaatctgc tcgacaatgc tataaaatac
     1441 accacggatg gcagtatcac cattgcgctc gagcgaaagg aagatggatg cgccatcgtg
     1501 gtatccgaca ccggccgcgg catgacggcc gacaaaataa aacagctcga ggactatgcg
     1561 caacagccgc atcaccagtc ttccgagacc tttggctatc gctttatctt ctcactggcg
     1621 gaaaagatcg gcgccggcat ccggatatcc agcgagccgc gaaagggtac aaacgtgacc
     1681 atttttatcc ccgggcttaa tgaggcacga ctcctggctt aatgatgcac ggcgccgcgc
     1741 ccaatgatgc gcgatgccgc gcccaacgat gcaaggctct tatagcatct tctgcaccca
     1801 ctccttcgtc gcacttacca gcaacgccat cacatccttt ttccccgctt tgaacatatg
     1861 attagccccc tcgatcttca ccaacgttgc ctttggcagg gacgcacaca ccgactctat
     1921 cagatcccat gtggctaatt catctttcgt gccctgcaaa aaaagcatct tcacctttac
     1981 cgccttcaaa tgctccgccc gttcgatgga aggcttgccg gaagggtgta acgggaaccc
     2041 gtaaaaaacg atccccttca cctcgggccg ggcatgggcc gccaggtact gggaagtcat
     2101 ccgtcctcca aaggatttgc ccgcggcgaa aagcggcagg gagggaaatg atccatgcgc
     2161 atgggtaatg gccgccgcga tggtttcgtg cgccacggcg ggagtatccg gcctcccttt
     2221 cctgtgctcg gtgaaaggga aattgaaccg cagggtcgcg atacccgcct ccgccagcgc
     2281 cgaagccaac gctaccataa aggaatggtc catgcctgcg ccggccccat gcgccagcgt
     2341 cacgatgcat ttactcgctt cgggaatgaa atactcggcc gaaaccgtgc cgatggacgg
     2401 agaaacctga aatgtgatgg atcgggtgtt catactacca atggatataa ggtcaaatgt
     2461 atattttata ctggtataat atttgattcc ttttatgtag cggcagaata atgaccctct
     2521 ttacaaaatt attttatggc tgttcattcc gggcaaaggc cattccccgc agtggtcatt
     2581 gcatccattc tattgattat ttgcgttttc ccgggccaac aagggatggc gggtctcctt
     2641 cccgggatat ccgggctggc ggcgatcagt ccgggcctta ccttccttga tatcccgctc
     2701 cgcctgcctc ttgcgatcga tctgatcctg gtgccgggcc tttttctcct ggcttatatg
     2761 atcatactcc tggtctatgc ttcccggcgg gatgggtcca ccgggtttgc gcaacgattg
     2821 ggagctgttt ttgtgggagg atttattgta ttgttttgca cggcattcgg attcctgctc
     2881 acttatctca tgcaggatca tcttccgctg tccgttcgaa gaggaatgaa cagttttgga
     2941 ataagcgccg acctttatct tcccagcgca aggtataaaa ccatccacct gcaggggaat
     3001 tttatagcgc tgctatgctt tatcattggg atagcattgt gcatccggaa aataaaagcg
     3061 ccgccggtta tgcagaaagt agcgccattg acgcgtgaac agcggatgac gccctatgag
     3121 cggatgatgg aggagaagcg gaaaacggcg cccgccgccc caaaaacacc aaaaacccca
     3181 aaaaaaccat ccccggtcca aaggaagcca tccgccgccc atacccataa cggccatacc
     3241 cgaaacctgc gtactccgtg ctacaaccaa cccctgctga gcatcgagcc ggaagccgtc
     3301 aactatatgc cgatgtaggg cgccgcatca ctccgtaaaa gcatccgcat caccccgtaa
     3361 gagcatccgc atccctccgg aaaagcgtct gcatcactcc aggaaagaag tggtcttccg
     3421 caacccgccc tcccagccaa gagatagata ataaacgcct tttgaaagcg ccggcagcgt
     3481 cagcgggata tagttgtccc cgggagtaag ggtggccccg ccggtccagc aaacccggcc
     3541 cgacgcatct ataacagcga gggaagtccg cgtcgcccgg tcggagacta tatacaacac
     3601 ccgttgtccc gcaccggcgg agcgaagcga aacctggcta ctgccatccg tcgatcgccg
     3661 aacggcaacc acctggctca ccaggggcgc attccctgtc tcatcgagct gtatccggta
     3721 aaagcccacc cgggttacag ccgtccgatc gacgaacgta tagcgatcct ggccggtaag
     3781 gacatcgaca tttccgagat cagagaaggc aatcccatcg gtcgaacgct gaacatggaa
     3841 tgccgttaga gacgtgacgc cggtgacgtc ccatgtcagg gtcaccgtat gatcgtcggc
     3901 gaccgtggcg gtaagatcca gcagctgcgc cgccagcgtg acaaaactgc cg
//
LOCUS       DATGFG010000025         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1133752, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000025 DATGFG010000000
VERSION     DATGFG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133752"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VKQ52_00465"
     CDS             <1..1446
                     /locus_tag="VKQ52_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ85670.1"
                     /translation="NEFIDFKDEPLLPYQLSREGPALAKADVDGDGLEDVFFGGAIGQ
                     GGQLFLQTQDGHFQPAPSQPWTADTVYEEVNALFFDANGDGHPDLYIVSGGNEYADSS
                     AGYQDNLYLNDGKGHFTKAPADALPVMNSSKFAIAAGDFNHDGRIDLFIGGRGAPGSF
                     PLPSKSYLLRNDSHDGIVKFTDVTEAACPDLRLPGMVKAAVWSNLDDTQYPDLLIAGD
                     WMPPRLFRNDKGKLTDVSAAAGLANLDGMWSSITAADVDGDGRTDFILGNCGLNNQFR
                     ASAAQPLSIYVADFDDNGSLDPILCYYIQGKSYPMASRDELLDQIVSLRKKFNSYKAY
                     ADATIEDIFPQEKLAKAKVLHCNELASGILYNKGNGKFVFSPLPLPAQFSKIFGAILD
                     DFDGDGKKDILVSGNFFPYRTQLGQCDASLGILLKGPGPSFRPVDPAISGCYIGGDVR
                     GMVELKGPAGERWVIIAKNNDAVQVIKVNSR"
     gene            1443..>2229
                     /locus_tag="VKQ52_00470"
     CDS             1443..>2229
                     /locus_tag="VKQ52_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85671.1"
                     /translation="MKVIITILAAWLAIGLPATPAAAPTAAQHPATASNPSTTQHRAT
                     AATPAAAQQTTPATAPPSAIAQHPAPAQHPAAAQDPAEPLRRAEAAFTDIMVHDIFSP
                     PVASRIYLYTNIAAYETLVKAHPQQYSSLHGQVKGFPEIPAPKKTISFPVASVYAFLL
                     VGKKLVFSDSAMQDSIHAILGSLRAGAPDAAGYKASLEYGQQVAAAIIAWAETDQYKE
                     TRRLPRYRISRQKDKWIPTPPVYMAAVEPYWNRIRPVTLDSAGQ"
BASE COUNT          493 a          789 c          546 g          401 t
ORIGIN      
        1 aatgaattca tcgacttcaa ggacgaaccg ctgctgcctt accaactgtc ccgcgaaggc
       61 ccggcgctgg ctaaagcgga cgtcgatgga gatgggctgg aagatgtctt cttcggcggc
      121 gccatcggcc agggcggaca gctgttcctc cagacacaag acggtcattt ccaaccggcg
      181 ccgtcccaac cctggacagc ggataccgtc tatgaagagg tcaacgccct cttcttcgac
      241 gctaatggcg acggacatcc ggatctctac atcgtcagcg gcggcaacga atacgccgac
      301 tcctctgccg gctaccagga taacctctac ctcaacgacg gcaaaggcca tttcacaaaa
      361 gcacccgcgg acgccctccc tgtcatgaac agcagcaaat tcgccatcgc cgcaggcgat
      421 ttcaatcacg atggccgtat cgacctcttt atcggtggcc gcggcgcccc gggctccttc
      481 ccgcttccct caaaaagcta cctgctgcgc aatgactcgc acgacggcat agtcaaattc
      541 acggatgtga cggaagccgc ctgcccggac ctccgcctgc ccggcatggt aaaagccgcc
      601 gtctggtcga atctcgacga tacgcaatac cccgacctcc tcatcgccgg cgactggatg
      661 ccgccacgac tcttccgcaa cgacaaggga aaattaaccg atgtctccgc tgcagcggga
      721 ctcgccaacc tggacggcat gtggtcttcc atcaccgccg ccgatgtcga tggcgacggc
      781 cggacagact tcatcctggg caactgcggg ttgaacaatc aattcagggc ttccgccgcc
      841 cagccccttt ccatctacgt tgccgacttc gacgacaacg gctccctcga tccgatcctt
      901 tgctactata tccagggaaa gagctatccc atggcctccc gggacgaact actggaccag
      961 atcgtatccc tgagaaagaa attcaatagt tataaggcct atgccgacgc gaccatcgaa
     1021 gacattttcc cacaggaaaa gctggcgaag gccaaggttc tgcattgcaa cgaattggcc
     1081 tccggcatcc tctacaacaa gggcaacgga aaatttgtct tcagcccctt accgctgccc
     1141 gctcaattct caaagatctt cggcgccatc ctggacgatt tcgatggcga cggcaaaaag
     1201 gatatcctgg tgtcgggtaa tttctttccc tatcgtaccc aactgggcca atgcgatgcc
     1261 agcctgggca tactcctcaa agggccaggt ccatccttcc gccccgtgga ccctgccatc
     1321 agcggctgct acatcggcgg cgatgtccgc ggaatggtcg aactaaaggg gccggccggc
     1381 gaaagatggg tgatcatcgc aaagaacaac gatgccgtac aggtcataaa agtaaattcc
     1441 cgatgaaagt cattatcacg atcctggcag cctggttagc cattggcctc cccgccactc
     1501 ccgccgccgc ccccaccgca gcacaacacc ccgcaactgc gtcgaacccc tcaaccacgc
     1561 agcaccgcgc cactgccgcc actcccgccg cagcgcaaca aaccactccc gccaccgcgc
     1621 cgccctcagc cattgcgcaa caccccgccc ccgcgcaaca ccccgccgcc gcgcaggacc
     1681 ctgccgagcc cctacgccgc gcggaagcgg ccttcaccga tatcatggtc cacgacatct
     1741 tctcgccccc cgtggccagc cgaatctatc tgtacacgaa tattgcggct tatgagaccc
     1801 tcgttaaagc gcatccccaa caatactcct ccctccacgg ccaggtgaag ggcttccccg
     1861 agatccccgc acccaaaaag acgatatcct ttcccgtcgc ctccgtctat gccttcctgt
     1921 tagtaggtaa aaagctggtc ttttcggact ccgccatgca ggactccatc cacgcgatac
     1981 ttggctcgct ccgcgccggc gcccccgatg ccgccggcta caaggcttcg ctcgagtatg
     2041 gccagcaggt ggccgctgcc atcatcgcct gggcggaaac cgatcaatac aaggagaccc
     2101 gccggctgcc gcgctaccgt atctcccggc aaaaggacaa atggatcccg acaccccccg
     2161 tctatatggc ggccgtagaa ccctattgga acaggataag acccgtcacg ctcgacagtg
     2221 ccggccagt
//
LOCUS       DATGFG010000026         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1250376, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000026 DATGFG010000000
VERSION     DATGFG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250376"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VKQ52_00475"
     CDS             <1..1447
                     /locus_tag="VKQ52_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ85672.1"
                     /translation="YQQLDTNFYKTHSTGDLMSRMAEDVSRVRMYTGPAVMYLANLLV
                     TIGFSLFFMLRKDALLSLYVLTPLPILAITIYYVNTIIHKKSEHIQALLSNLTTNAQE
                     SYSGIRVIKSFVQEKAMLRFFSHNSEEYRKNAVNLAKVEAIYFPAMGLMIGLSTLLAI
                     MIGGLYVIYGKHNTDIGTITEFVIYITMLTFPVSAIGWVASMVQRASASQKRLNEFLD
                     TEPVIQNAPGANAMPLQGNISFQGVDFTYPNTGIHALADFNLEIRKGEKVVIIGRTGS
                     GKTTIAQLLLRMYDPDKGRVLIDGTDLRELNLQDLRRQVSYVPQDVFLISDTVSGNIR
                     FGLDHTDEDKIRTAARYASVDKEIDGFAHRYETMIGERGVTLSGGQKQRISIARGLIK
                     DPEIIVFDDCLSAVDAKTEKEILGNLYQYLQHKTAIIITHRIFSLFDFDRIVVLEEGR
                     IVESGTHQQLIALNGYYTYLYEQQQAENSPA"
     gene            1587..2024
                     /locus_tag="VKQ52_00480"
     CDS             1587..2024
                     /locus_tag="VKQ52_00480"
                     /inference="COORDINATES: protein motif:HMM:NF023109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HLZ85673.1"
                     /translation="MAYENNDKRMESVYSKRIRAGKRRTYFFDVRATRSNDYYLTITE
                     SRKKFNEDGYDRHKIFLYKEDFNKFLKALTEAVDYVKTELMPDFDFDAYNHDNVQENE
                     GGYQEAVSDDVIEEDVRPAAHTHTPTAGTHTPTPTTGEEVDKW"
     gene            complement(2124..>2715)
                     /locus_tag="VKQ52_00485"
     CDS             complement(2124..>2715)
                     /locus_tag="VKQ52_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85674.1"
                     /translation="FEGQEAEDPARKVGIAAFKVYYFATGQLQENAFPHKRFAERRDW
                     PHFDAAYFSGCAHAIRRSVFEEVGFYPEDFFYGMEEYDLSYRAIEAGYSIRYDDRVVV
                     LHKESPAGRLTPREKIRGMWVNKSKVAWKYLPLRFFYTTAFLWSLQYLKKSGWHVRGF
                     FRGWEDVNRIQATERRSPVGSAARNYLRRVKARLSY"
BASE COUNT          689 a          809 c          653 g          564 t
ORIGIN      
        1 ctatcagcag ttggatacca atttttacaa aacccatagc acgggcgatc tgatgagccg
       61 catggcggaa gacgtcagcc gcgtccgcat gtataccggt cccgcggtca tgtacctggc
      121 caacctcctc gtgaccatcg gcttcagcct cttcttcatg ctccgcaagg atgccctgct
      181 cagcctctat gtgctgacac ccctccccat cctcgccatc accatctact acgtcaacac
      241 catcatccat aaaaagagcg aacatatcca ggccctgctg agtaacctca ccaccaacgc
      301 ccaggaatcc tatagcggca tccgggtgat aaaatccttc gtccaggaga aggccatgct
      361 tcgcttcttc agccacaaca gcgaagaata ccggaagaac gccgtcaatc tcgccaaggt
      421 ggaagccatc tattttcccg ccatgggtct gatgatcggc ctcagtaccc tcctggccat
      481 catgatcggc gggctgtatg tcatctatgg caagcataac acggatatcg gcaccatcac
      541 cgagttcgtc atctatatca ccatgctcac cttccccgtc agcgccatcg gctgggtggc
      601 aagcatggtc caacgggctt ccgcctccca gaaacggctc aacgagttcc tcgataccga
      661 acccgttatc caaaacgccc ccggcgccaa tgcaatgccc ctacagggaa acatcagctt
      721 ccagggagtg gatttcacct atccgaatac aggtattcac gcactggcgg attttaatct
      781 ggagatccgg aaaggggaga aggtcgtcat catcggcagg accggcagcg gcaagaccac
      841 catcgcccaa ctcctgctac gcatgtatga ccccgacaaa ggcagggtac tgatagacgg
      901 taccgatctg cgggagctga acctccagga cctgcgtcgc caggtcagct atgtccccca
      961 ggacgtattc ctgatcagcg acaccgtctc gggcaatatc cggttcggac tcgaccatac
     1021 agacgaggac aagatccgca cggccgcccg ttacgccagc gtggacaagg agatcgacgg
     1081 cttcgcccat cgctacgaga ccatgatcgg cgaacggggt gtgacgctca gcggcggcca
     1141 gaaacaacgg atatccattg cccgcggcct tatcaaggac ccggagatca tcgtattcga
     1201 cgactgtctg tcggctgtcg acgctaagac tgaaaaggaa atacttggta atttatacca
     1261 atatctgcaa cataaaaccg cgataatcat tacccatcgc atcttttccc ttttcgattt
     1321 cgatcgcatc gtcgtgctgg aagagggccg gatcgtggaa tcgggcaccc accagcagtt
     1381 gatcgccctc aacgggtatt acacctatct ctatgagcag caacaggccg agaacagccc
     1441 ggcatagtgt ccggttccga tgcacacgca cggactacgt gtaaattggg cgtaaatagg
     1501 tgctataatt attttggcaa attttgctaa ttcgaaaaca atactatatt tgtgactgct
     1561 ttattagtaa ttaaaacaaa atcattgtgg cgtacgaaaa caacgacaaa cgaatggaaa
     1621 gcgtctacag caaaaggatt cgcgctggca aacgcaggac gtatttcttc gatgtgaggg
     1681 caacccggag taacgattac tatcttacta tcaccgaaag ccgtaagaaa tttaatgagg
     1741 atggttacga caggcacaag atcttcctgt acaaagagga cttcaacaag ttcctgaaag
     1801 cgttgactga ggcggtggat tatgtgaaaa ccgagctgat gccggatttc gacttcgacg
     1861 cctataacca cgataacgtc caggaaaatg aaggcggata ccaggaagca gtaagtgatg
     1921 atgtgatcga ggaggatgta cggcccgctg cgcatacgca tacgcccacc gccggcacgc
     1981 atacccccac tccgaccaca ggggaagaag ttgacaaatg gtaacccacc ctcaccggta
     2041 acgatactaa aaagacctcc tactttgtag ggggtttttt tatgcagtct tttttatgcg
     2101 gagtctttta gtcgatagcg tattcaataa ctcaatctcg ccttcacccg ccgcaggtaa
     2161 ttccgggcgg cgctgcccac cggcgaccgc cgctccgtcg cctgtatccg gttaacgtcc
     2221 tcccatcccc tgaaaaaacc gcggacatgc cagccgctct ttttcaggta ttgcagactc
     2281 cagagaaatg ccgtcgtgta aaaaaagcgc aacggcaggt acttccaggc caccttgctc
     2341 ttgttcaccc acatcccccg gatcttctcc cggggcgtca gccgccccgc cggggactcc
     2401 ttatgcagga caacgaccct gtcatcatac cggatcgaat aacccgcctc gatggcgcgg
     2461 tagctgagat catattcttc catcccataa aagaaatcct ccgggtaaaa cccaacctcc
     2521 tcgaacaccg aacgccggat ggcgtgagcg cagccggaaa aataggcggc atcgaaatgc
     2581 ggccagtccc tgcgttcggc aaaccgcttg tgcgggaagg cattctcctg cagttgcccc
     2641 gtggcaaaat aatacacctt gaaagccgcg atcccgacct tccgggccgg gtcttccgcc
     2701 tcctggcctt cgaaa
//
LOCUS       DATGFG010000027         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1024176, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000027 DATGFG010000000
VERSION     DATGFG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024176"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VKQ52_00490"
     CDS             complement(<1..199)
                     /locus_tag="VKQ52_00490"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HLZ85675.1"
                     /translation="MRRTILITTLGISLPAILLGQKQPLRLWYDKPARQWEETLPLGN
                     GRLGMTPDGGVTEEKVVLNDIT"
     gene            complement(223..1455)
                     /locus_tag="VKQ52_00495"
     CDS             complement(223..1455)
                     /locus_tag="VKQ52_00495"
                     /inference="COORDINATES: protein motif:HMM:NF027705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4998 domain-containing protein"
                     /protein_id="HLZ85676.1"
                     /translation="MKTIYKNNWIWLICCLTLWMDCTRKPTDYRSFLNGQEITYPGKI
                     INPNVATGNLRLLLTWMPSPDPSITKYVVYWNNYADSTIVTATSHSPSDTVKCLITNL
                     AEYSYTFFIYSYDNKGNRSVLTELDNAKVDGPIYQSGLHNRPLDPAKPAIVNPDSSAT
                     LYFLAPYDTINITTRIRWVRMAGDTVTNYLPPASDSIVVPLFKRGTKVLVQSSFLPNR
                     SAIDSFYTLSPDTAAPYKPIIYIMCDKSKFKPVNLPNDLVAGFGTTLPTIWDGNMQPR
                     GYPSVFHSDGSGTPPLPGTITMDMGQVYNSLAKVEETGRTCCHNPQEFEVWGIADTTG
                     AISPLLPSNSGWKADMTTRGWTLLGDIIRADDGVAPYDALLQSNPPPVRFLIVRFVSD
                     VDNSGYINLSQMTFWYLQ"
     gene            complement(1498..2763)
                     /locus_tag="VKQ52_00500"
     CDS             complement(1498..2763)
                     /locus_tag="VKQ52_00500"
                     /inference="COORDINATES: protein motif:HMM:NF027707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4959 domain-containing protein"
                     /protein_id="HLZ85677.1"
                     /translation="MQKIGKIICLSIVAGALALSQPGCKKTDGYNTVTSTDKTKPGPV
                     TNVKVVNFNGGAYITYSLPDSKNILYVQANYAINDKITRQTKSSYYSDSITVSGFAKS
                     QDYKVTLQVVSRAEVKSDSVVVTVHPDTPPYLLTLPTLKMQPDFGGVNLVALNKVKAD
                     IGIILITPNATTNRLEIINQDYTNADSVNISVRGYDTIPRQFGLYVTDAWGNISDTLF
                     ATIKPIYEVQMPKSGFSAFVLSSDVAVYDSYRGLQNLWDNNIKEPTYNTQQPIQPLVW
                     PAWMTFDMGLAAKLSRYTMVGRTGDNGPGEFMWGYGTPQTWVIWGRADVPVDEQMPDS
                     THLPLVGQATPGGWINMGEFYAPPKPSGLPSPQFTSADLAFWQKGFPFNFALTLPKVR
                     YIRFECLTNMGGTNNFFDINELTFFGDPR"
     gene            complement(2782..4707)
                     /locus_tag="VKQ52_00505"
     CDS             complement(2782..4707)
                     /locus_tag="VKQ52_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ85678.1"
                     /translation="MKIFIKLLFAGLPVLLVASCKKYLNVTPDNVATLSSSFSNANET
                     EAYLFGCYNAMQQLGDVRHNAGFTTSGEVIFPINLNDRTTLGGQGGDAGFTIMQGLQN
                     AANPLLNFWDGYNMGLNLWQSIRMCNTFLANVNTPLDLPAYQKARWVAEAKFLKAWYH
                     YWLIRMYGPIPIMDVALPINATSDQVRTPQQSVDSCFNYVVHLLDTAIAGLPPTIQNA
                     AAEQGRITSAIALAVKAEVLATQASPLFNGNPDYAGMKGKDGKPLFSASADPAKWQRA
                     LDACNAALTAANAAGATLYQLKLNGNVTHMSDSTKKLLTIQGAITDSWNPEQIWSLNF
                     QYNWQYMTAARVTADAAANVFAVYSNFSVPVAESELFYTNNGVPINEDITWDYANRYQ
                     LQKGDSAHVYYIKKGYTCPKGNFNRENRYYADVAFDGSIWFGAGNTDDNNPNYVNAVN
                     GFAAAPDALRINVTGYWAKKLVPWQSVFGQTSVNLNYMWPMIRLPALWLLYAECLNEV
                     NGPGAQAYQYIDMVRARAGLQGVQASWAAYSNNPNKPNTKDGLRQIIHQERRIELAFE
                     GQAGWDLRRWKELQTVLTGPIQGWNTNPGNNGRTVTGYYQLVNYYQPVFGLRNYIYPI
                     QTYDLLINPNLVQSLYW"
     gene            complement(4724..>5776)
                     /locus_tag="VKQ52_00510"
     CDS             complement(4724..>5776)
                     /locus_tag="VKQ52_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85679.1"
                     /translation="YPNPDVTWETAKKSNVAVEMTVAKDFNIIAELYHEYRYNILIPR
                     GYIPVSVGIESSAQANLQANLGTAYSTGVDVHVDYNRTLSKDLAITLLGNFTYSTNKV
                     GHLEEPQYPYSYLFHSGHPIDQQFGFIGERLFVDDKEAANSPTQSFSGPLPQGGDIKY
                     RDVNKDGVIDGYDRVPVGLPTTPEIIYGFGFSLKYKQFDLNAFFQGLARESFFLNSSA
                     QDDRYYGHYGTEPFVNNAQLLRAYANNHWSLENQNLYALWPRYSLYDNVNNDQQSSWW
                     LRDGSFMRLKSLEVGYTFPKKWTKHAYMDNARLYFSGLNLLTFSHFKLWDPEQAGQGF
                     GYPIQKVINVGLNLNF"
BASE COUNT         1229 a         1439 c         1651 g         1457 t
ORIGIN      
        1 gcgtgatatc gttgagcacg accttttctt ctgtcacgcc gccgtcaggc gtcatgccca
       61 ggcggccatt gcccagcggc agggtctctt cccactgtcg tgccggcttg tcgtaccaca
      121 ggcgcagggg ttgtttttgc ccaagcaata tagccggcaa ggagattcca agggtcgtga
      181 tcaggatggt tcttctcaaa atcataaagt aggttaattg gcttactgca ggtaccagaa
      241 ggtcatttgg cttagattga tatatcctga gttgtccacg tcactgacaa accggacgat
      301 gaggaatcgc acaggcggcg ggttgctctg caggagggcg tcatacggag ccacaccatc
      361 atctgcccgg ataatgtctc ccagtaaggt ccatcccctg gtggtcatat ccgctttcca
      421 tccgctgtta ctcggcagca gcgggctgat cgccccggtg gtatctgcga tgccccacac
      481 ttcgaattcc tgtgggttat ggcagcaggt acggcctgtc tcttctactt ttgccagcga
      541 attgtacacc tggcccatgt ccatggttat cgtgccaggt aagggcgggg tgccgctgcc
      601 atcggagtgg aacacactcg gataccccct gggctgcata tttccgtccc atatagtcgg
      661 cagtgttgta ccgaaaccgg ccaccaggtc gttcggaaga ttaaccggtt tgaacttgct
      721 cttgtcacac atgatgtata ttatgggctt atacggagcg gccgtgtccg gcgacagggt
      781 gtagaacgag tcgatcgcgc tccggttcgg gagaaaagaa gactggacca gtacctttgt
      841 tcccctctta aaaaggggca cgacgataga gtcactcgcc ggcggcagat aattggtcac
      901 cgtatccccg gccatcctca cccaccggat acgggtggtt atgttgatcg tatcgtatgg
      961 cgccaggaaa tagagggttg cggagctgtc gggattgacg atcgcgggtt tggcgggatc
     1021 gagcggccgg ttgtgcaatc ccgactgata gatcgggccg tccacttttg cgttgtccag
     1081 ttcggtcagg acactccggt tgcccttgtt atcatacgag taaataaaaa aggtgtacga
     1141 atattcggcc aggttggtga tcaggcattt caccgtgtcc gagggggaat gagaggtggc
     1201 ggtcacaatc gtggagtcgg catagttatt ccagtagacc acgtattttg ttatgctggg
     1261 atcggggctg ggcatccagg tgagcagcag gcgcaggttg ccggtagcga cgttcgggtt
     1321 gatgatctta ccgggatagg tgatctcctg gccattcaga aaagaccggt agtcggtggg
     1381 tttcctggtg cagtccatcc atagggtgag gcagcagatc agccagatcc agttattttt
     1441 ataaatcgtt ttcattgcaa tttatttagt ggtttatggc ggtgtggccc tgagggtcta
     1501 tcgcggatcg ccaaagaatg tcaattcatt tatgtcaaag aaattattcg tcccgcccat
     1561 gttcgtaagg cattcgaaac ggatgtaccg gactttgggt aatgtcaatg caaagttgaa
     1621 agggaatcct ttctgccaga aggccaggtc cgcggaagta aattgggggc tgggaaggcc
     1681 cgaaggtttg ggtggggcgt agaattcacc catattgatc cagccgccgg gtgtcgcctg
     1741 acccaccagg ggaaggtggg tgctgtccgg catctgttca tccaccggca cgtccgcccg
     1801 gccccagatg acccaggttt gcggcgtccc gtagccccac atgaactctc cggggccgtt
     1861 atcgcccgtc ctgcctacca tcgtataacg gctgagcttg gccgcaagac ccatatcaaa
     1921 ggtcatccag gcgggccata ccagcggctg gatcggttgc tgcgtgttat aggtgggctc
     1981 cttgatatta ttatcccata aattctgcag gccccgatag ctgtcataga ccgcgacatc
     2041 cgaagacaga acgaaagcac taaacccgga tttgggcatc tgcacttcat aaatgggctt
     2101 tatagtcgca aacagcgtgt cggagatgtt tccccatgcg tcggtgacat acaggccgaa
     2161 ctgtcgcggg atagtatcat atccccttac actgatattc acggaatcgg cattggtgta
     2221 atcctggttg atgatctcca gtctattggt cgtagcgttg ggagtgatca ggatgatgcc
     2281 gatatcggct tttaccttat taagcgcaac caggttgaca ccgccgaaat cgggctgcat
     2341 tttaagggta ggtagtgtta gcaggtaagg cggggtatcg ggatgtacgg tcaccaccac
     2401 ggagtcggac tttacttccg cgcggctgac gacctggagt gtcaccttat agtcctggct
     2461 ctttgcgaaa ccgcttacgg taatgctatc cgaatagtag gatgatttcg tctgccgggt
     2521 tattttatca ttgatggcat agttggcctg gacatagaga atattctttg aatcgggcaa
     2581 cgaatacgta atatacgcac cgccgttgaa gttgacgacc ttcacattgg taacgggtcc
     2641 cggcttggtc ttgtccgtag aggtaacggt gttgtaaccg tctgtttttt tacagccggg
     2701 ctggctcagg gccagggcac ccgcaactat tgataaacaa atgatcttac ctattttttg
     2761 cataaactgc gtttagtgtt tttaccaata aagactttgc acgaggttgg ggttgatcag
     2821 cagatcgtag gtctgtatcg gataaatata gttcctcagg ccaaacaccg gctggtagta
     2881 gttaaccaat tggtaataac cggtaacggt ccggccgttg ttaccggggt tggtgttcca
     2941 accctgtatg gggcctgtga gtacggtctg caattccttc catcgtctga ggtcccagcc
     3001 ggcctgtcct tcgaaggcaa gctctatccg tcgttcctgg tggatgatct gtcttagtcc
     3061 atcctttgta ttgggtttgt tcgggttgtt ggagtatgcc gcccaggaag cctgtacgcc
     3121 ctgcaggcct gctcttgccc tgaccatgtc gatgtattga taggcctggg cgccgggccc
     3181 gtttacctcg ttgagacatt cggcatacag gagccagagg gccgggaggc ggatcatcgg
     3241 ccacatataa ttcaggttta cgctcgtctg accaaaaacg gattgccagg ggaccagctt
     3301 ttttgcccaa tagccggtga cgttgatccg cagggcatcg ggcgccgccg cgaagccgtt
     3361 gacggcgttc acatagttgg ggttattgtc gtcggtattg ccggcgccga accagatact
     3421 cccgtcgaag gcgacatcgg catagtaccg gttctcgcga ttgaaattcc ctttcggaca
     3481 ggtgtatcct ttctttatat aataaacatg tgcactgtcc cctttttgca actggtaccg
     3541 gttcgcataa tcccaggtga tatcttcatt gatggggacg ccgttgttgg tatagaagag
     3601 ttccgattcg gcaaccggta cggagaaatt ggaatagacg gcgaatacgt tggcggccgc
     3661 atctgcggtg actcttgcgg ccgtcatgta ctgccagttg tattgaaagt tcagggacca
     3721 gatctgctcg gggttccagc tgtccgtaat ggccccctgg atggtcagga gctttttggt
     3781 gctgtcgctc atatgcgtca cattaccgtt gagtttcaac tgatacaggg tggcgccggc
     3841 agcatttgcg gcagtgaggg cggcattgca ggcatccagg gcccgctgcc attttgcggg
     3901 gtccgctgaa gccgaaaaga ggggtttccc atccttgccc ttcataccgg catagtcagg
     3961 attgccattg aacaggggac ttgcctgtgt agccagcacc tcggccttaa ccgccagggc
     4021 gatggcggag gtgatcctgc cctgttcggc ggccgcgttc tggatcgtcg gtggaagacc
     4081 ggcaatagcg gtatccagca ggtgaaccac ataattgaaa caggaatcca ccgactgctg
     4141 cggggtcctt acctgatcgg aggtggcatt gatcggcagg gcgacatcca tgatcgggat
     4201 cgggccatac atgcggatca gccagtaatg gtaccaggcc ttcaggaatt ttgcttcggc
     4261 gacccatctt gctttctggt aggccggcag gtccagcggg gtgttcacat tggccagaaa
     4321 agtattacac atccggatgg attgccagag gttcaggccc atattatacc cgtcccagaa
     4381 gttcagcagc gggttggcgg cgttctgcag tccctgcatg atcgtgaacc cggcgtctcc
     4441 gccttgtccg ccgagggtgg tccggtcgtt gagattgatg gggaagatca cttcgccgga
     4501 ggtagtaaac ccggcattat gccttacatc gcccagttgc tgcatggcat tataacagcc
     4561 aaagaggtaa gcttccgttt cgttggcgtt ggagaacgaa ctgcttagtg tagccacatt
     4621 gtcaggggtc acattcaaat attttttgca ggaagcgacc agtaagaccg ggaggcctgc
     4681 aaacaatagt tttatgaaga tcttcatgat tcttttttta gacttagaaa ttcaggttca
     4741 aaccgacatt gatcactttc tggatcggat agccgaatcc ctgtccggcc tgttccgggt
     4801 cccacagctt gaaatggcta aaggtcagga ggttcagccc gctgaaataa agccgggcat
     4861 tatccatata ggcatgcttt gtccatttct tcgggaaggt gtaccccacc tccagggatt
     4921 tcaatcgcat gaagctgccg tcccgcagcc accaggaact ctgctggtcg ttgttgacgt
     4981 tgtcgtaaag agaataccgc ggccaaaggg cgtacaggtt ctggttctcc aggctccagt
     5041 ggttattcgc gtaggccctt agcagttgcg cattgttgac gaatggctcg gttccataat
     5101 ggccataata tctatcgtcc tgcgccgagg agttcaggaa gaaagattct cttgccagcc
     5161 cctggaagaa ggcattcagg tcgaattgtt tgtacttcag cgaaaatccg aagccataga
     5221 tgatctccgg tgtggtgggc aggccaacgg gcaccctatc ataaccgtcg atgaccccat
     5281 ccttgttgac gtccctgtat ttgatatcac cgccctgggg cagggggccg ctgaaggatt
     5341 gggtcggtga attggcggct tccttgtcat ccacaaaaag ccgttcgccg atgaacccga
     5401 attgctgatc gatgggatgg cccgagtgga aaagatagga atagggatat tgcggttctt
     5461 cgaggtgacc gaccttattg gtcgaatagg tgaaattgcc cagcagggtg atggcaagat
     5521 cctttgatag tgttctatta taatcgacgt gcacatcgac acccgtcgaa taggccgtac
     5581 ccaggttggc ctgcaggttg gcctgagcgc tgctctcgat acccacggaa accgggatat
     5641 aaccgcgtgg gatcaagata ttgtaccggt attcatggta aagctcggcg ataatgttga
     5701 agtccttcgc taccgtcatt tccacggcca cattgctctt tttggccgtt tcccaggtaa
     5761 cgtccgggtt gggata
//
LOCUS       DATGFG010000028         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1023935, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000028 DATGFG010000000
VERSION     DATGFG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023935"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VKQ52_00515"
     CDS             complement(<1..483)
                     /locus_tag="VKQ52_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85680.1"
                     /translation="MLRTFATILFVCASAAAGLAQPFPSFEKKELLKGNDTLRYRIQY
                     PLGYDAMKKYPLILLMHGAGERGSDNEAQLTWGGSLFADSAVRAKYPAIVVFPQCAAT
                     DFWANLTYNPTDSIRFHMRSDTAARPSMGLVLQLLDELAAGGHVDTKRIYVGGLSMGG
                     M"
     gene            596..>3882
                     /locus_tag="VKQ52_00520"
     CDS             596..>3882
                     /locus_tag="VKQ52_00520"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HLZ85681.1"
                     /translation="MSIRIAINHRTTYQYDRQVSLSPHVFRLRPAVHSRTPIEAYSFK
                     VSPGDHFINWQQDPFGNFQARVVFPEKTTKLDIEVEVIAEMVVINPFDFFVEEDAQKF
                     PFTYKAILAGELVPYLETTESGPLLDQWMQRIDRSPKNIIDFLVYINQRVNQDIAYTL
                     RMEAGVQTCEETLTRALGSCRDSAWLLVQILRRLGLAARFVSGYLVQLRADVKSLDGP
                     SGPENDFTDLHAWTEVYIPGAGWVGMDPTSGLFAGEGHIPLACTPHYVSAAPVVGATD
                     KCETTFSFSNTVTRIHEDPRVTKPYSEEQWATIDATGYKVDEDLEAGDLRLTMGGEPT
                     FVSIDDMESAQWNTAADGSEKRLLAHDLIFRLRDRFGPEGMIHYGQGKWYPGEPLPRW
                     QYGLFWRKDGVPLWKNSRLIAHEKTCRGYTQGDAARFIQTLADHLGVSEENISPAYED
                     PVHLLWTEGKMPVNIDPARVNWKDPLERRTLYRLLDTGAGNPAGYTLPLKWDYQRGRW
                     KSAKWVLAREWVFLMPGNSPIGLRLPLDSLPLVAKEYEAQKVERSLFEELPPLGDYPS
                     LVNARYGQPAEPAAQMIAAQTFMPQTTARQGTMPSATQPQPGAHQFPTPPPRPQLTET
                     IKTALCAEVRDGILYIFLPPMDYLEHYLDAIACIEATAEKLNVPVRIEGYEPPRDYRI
                     ERLVVSPDPGVIEVNIQPANNWKELTRTIDTLYEQAFLSRLGTEKFMLDGRHTGTGGG
                     NHITIGGAKPADSPLLRRPDLLRSLITYWQHHPGLSYLFSSAFIGATSQAPRVDEGLS
                     EKLYELEIAFSQVPEGGFIPFYLVDRIFRHLLTDITGNTHRSEFCIDKLYSPDTSSGR
                     LGILEFRAFDMPPHRQMSLVQMLLIRALIAKFWKKPYKHGLVRWGTELHDKFLLPQFV
                     RHDLLEVVSDLNEAGYPFDISWFDPFVEFRFPRYGTVSLQGITMEIRMGIEPWNVLGE
                     EMSGSGTARYVDSSMEKLQVKLNGWNGDRYALLCNGRRVPLTATGVKEEFVCGIRYRA
                     WQPSSALHPTIGIDAPITFDIVDTWNSRSIGGCTYYVSHPGGRSYEAFPVNSNEAESR
                     RGNRFAVT"
BASE COUNT          830 a         1223 c         1075 g          754 t
ORIGIN      
        1 catgccgccc atggaaaggc cgccgacata aattcgtttg gtatccacat gcccgccggc
       61 cgccagctca tcgagcagtt gcagcaccag ccccatcgac ggcctggccg ccgtatccga
      121 gcgcatatgg aagcggatcg agtcggtggg gttataggtt aaattggccc agaaatccgt
      181 ggccgcgcac tgcgggaaca cgactatcgc cggatatttt gcgcgaaccg ccgaatccgc
      241 gaacaggctg ccgccccagg tgagctgtgc ctcgttgtcg ctgcctcttt cgcctgcccc
      301 atgcatcaac aggatcagcg ggtatttctt catcgcatcg taacccagcg ggtattggat
      361 gcggtaacgc agggtgtcgt tccctttcag gagctctttc ttttcgaagc tgggaaaggg
      421 ttgggcaaga cccgctgcgg cggaagcgca gacgaagagg atggtggcaa aggttctaag
      481 catcgggaat ggtttgtctg tccaagatag gcattccggg ggaacggcca tttcatgatt
      541 tttacctatc ttcagaagag attgctcgct attcaaccaa aacagccgcc accacatgtc
      601 gatccggata gccatcaatc acaggaccac ctaccaatac gaccggcagg tctccctctc
      661 gccgcatgtc ttccgcctga ggcccgccgt tcattcccgg acgcccatcg aagcctattc
      721 tttcaaggtg tctccaggag accattttat caattggcag caggacccct tcggcaattt
      781 ccaggcccgg gtggtcttcc cggagaaaac aacgaagctg gacatcgagg tcgaggtcat
      841 cgcggagatg gtcgtcatca atcccttcga cttctttgtc gaagaggatg cgcagaaatt
      901 tcccttcacc tacaaggcga tcctcgccgg agagctggtc ccctacctcg agacaacgga
      961 gagcggcccc ctgctggatc aatggatgca gcgcatcgac agaagcccca aaaacatcat
     1021 cgatttttta gtctacatca atcaacgggt gaaccaggat attgcgtaca ccctccgcat
     1081 ggaggccggt gtccaaacct gcgaagagac cctgaccagg gccctgggct cctgccggga
     1141 ctccgcctgg ctgctggtgc agatcctgcg gcggctggga ctggcggcaa ggttcgtctc
     1201 cggttacctg gtgcaactga gggccgatgt caaatcgctg gatggaccgt cgggaccgga
     1261 aaacgacttt accgatcttc atgcctggac ggaagtgtat atccccggcg ccggctgggt
     1321 cgggatggac cctacctcgg ggctgtttgc cggcgaaggc catatcccgc tggcctgtac
     1381 tccgcattat gtgagcgccg ctcccgtggt gggcgccacc gataaatgcg agaccacctt
     1441 ctccttttcc aataccgtga cgcgtatcca tgaagacccg cgggtgacca aaccctattc
     1501 ggaagaacaa tgggcgacca tcgatgccac cggctacaag gtggatgagg acctcgaagc
     1561 aggcgatctt cggctgacca tgggcgggga accgaccttt gtctccatcg acgacatgga
     1621 gtcggcgcaa tggaacacag ctgccgacgg atcggaaaaa cgactgttgg cccacgacct
     1681 tatcttccgc ctccgggatc gattcggacc cgaagggatg atccactacg ggcagggcaa
     1741 gtggtacccc ggcgaaccgc tgccccgctg gcaatatggc ctgttctggc ggaaggatgg
     1801 tgttccccta tggaagaaca gccgcctcat tgctcatgaa aaaacatgca ggggctatac
     1861 ccagggtgac gctgcccggt tcatccagac cctggccgac cacctgggag taagcgaaga
     1921 aaatatcagt cctgcctacg aagatcccgt tcacctttta tggacggaag ggaaaatgcc
     1981 cgtcaacatc gaccctgcca gggtcaactg gaaagatccg ctcgaacgcc ggaccctcta
     2041 ccggctactg gataccggtg cgggaaatcc tgccggctac accctgcccc tgaaatggga
     2101 ttatcagcgg ggccgatgga aaagcgcgaa gtgggtcctc gccagggagt gggttttcct
     2161 aatgccgggc aattccccga tcgggctgcg actgcccctg gactccctgc ccctcgtcgc
     2221 caaggagtac gaagcgcaaa aggtggaacg cagccttttc gaagagctgc cgcccttagg
     2281 cgattaccca tcgctcgtca acgcgcgcta tggacagccg gccgaacccg cggcgcaaat
     2341 gatcgcggcg caaaccttca tgccccaaac caccgcacga cagggcacca tgccatccgc
     2401 aacccagccc caaccaggag cacaccaatt ccccacgcca cccccgcgcc cgcaactgac
     2461 cgaaaccatc aagacggctc tctgcgcgga agtccgcgac ggcatcctct atatcttcct
     2521 cccgccgatg gactacctcg agcattacct cgacgctatc gcctgcatcg aagccacagc
     2581 ggagaaattg aatgtcccgg tgcgaatcga agggtacgaa ccgccccgcg actaccgcat
     2641 cgagcggctg gtcgtatccc ccgaccccgg cgtcatcgag gtcaatatcc aacccgccaa
     2701 caactggaag gaattgaccc ggaccatcga caccctctac gaacaggcct ttctatcccg
     2761 cctcggcacc gaaaaattca tgctggatgg ccggcatacc ggcaccggcg gcggcaacca
     2821 tatcaccatc ggtggcgcga aacccgccga cagccccctg ctgcggagac ccgatctgct
     2881 gcggagtctc atcacctact ggcagcatca tcccgggctc tcctatttgt tttccagcgc
     2941 cttcatcggc gccaccagcc aggcgccccg cgtggacgaa gggctcagcg aaaaactcta
     3001 tgagctggag atcgccttta gccaggtgcc cgaaggcggc tttatcccct tctatcttgt
     3061 cgaccgcatc ttccggcatc tgctgacgga tatcacgggc aacacccatc gcagtgagtt
     3121 ctgcatcgat aaactctatt cccccgacac ctcttcgggc cggctgggca tcctcgaatt
     3181 ccgcgccttc gatatgccgc cacaccggca gatgagcctg gtgcagatgc tgctcatccg
     3241 ggcgctgatc gcgaaatttt ggaagaagcc ctacaagcac gggctggtgc gctggggcac
     3301 cgagctgcac gataaattcc tgctgccgca atttgttcgc cacgatctgc tcgaagtggt
     3361 cagcgatctc aacgaagccg gctatccctt cgacatcagc tggttcgatc cctttgtgga
     3421 gtttcgcttt ccccgatatg gcaccgtctc cctgcagggt atcaccatgg agatccggat
     3481 gggtatcgag ccgtggaatg tgctgggaga agagatgtcc ggttccggca cggcccggta
     3541 tgtagattcc tccatggaaa aactgcaggt aaagctcaac ggctggaacg gcgatcgcta
     3601 tgcgctgctt tgcaatggcc ggcgcgttcc gctgacggca acgggagtga aggaagagtt
     3661 tgtctgcggc atccgctata gagcctggca gccgtcttcg gcgctgcatc ctaccatcgg
     3721 tatcgatgca ccgatcacct tcgatattgt ggacacctgg aacagccgga gcatcggtgg
     3781 ttgcacgtac tatgtgtctc atcccggcgg ccgtagctat gaggcctttc ccgtcaacag
     3841 caacgaggcg gaatccagaa ggggcaatcg attcgccgtc ac
//
LOCUS       DATGFG010000029         4061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1595821, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000029 DATGFG010000000
VERSION     DATGFG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595821"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VKQ52_00525"
     CDS             <1..627
                     /locus_tag="VKQ52_00525"
                     /inference="COORDINATES: protein motif:HMM:NF023179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HLZ85682.1"
                     /translation="PIGRHETYLAAGFQAEYYQSQLNVAGVSGAFGDQFDAYGPIEGA
                     PSADRLANGWSYGHFNFNAGVSLFSNGRNDKWYIGGSMMQVNQPYAYKIKSDSTRLKP
                     VISIQGGYKYTTKEEDEVGFYMSMSWEGPAYRHYYELIYTKAIPGIAGGAAVGLGLGY
                     RYNDALVPNVELRYQKLTLGILYDVNISTISASGIDRNGMELALKMDF"
     gene            638..2293
                     /locus_tag="VKQ52_00530"
     CDS             638..2293
                     /locus_tag="VKQ52_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ85683.1"
                     /translation="MKKVMIGLIGCSLLALTTARPQNSGRHAAGSHRPAADTTGQAKE
                     HTVITRDTLPPTTQDTMFRYRGDHDTTPAVKDTVISLADDAPAAPAVKKDTVTDPPKT
                     DPAKTDPPKTDAAKTDPKPADPANADSKTTPAEPIDTTAEDKAEQQKRELDRRWFIAP
                     LTKLQFQDFAMLEKNRKGYLSDANTLPFFQRGNASFAASAYKNLTNRLSISADIGLSF
                     GHATSNDVLISQTPSKTYNLLNAAIYYHLISPAYRLQPYVTVGFNDIINDKSYPTVPL
                     GIGAKFNARSIMVLGQVSYGQSISKNVASTTMYAVGIYIPIKSKKQKQLDDDDNSRLN
                     KKDKDEAKKKDTANKNNNGGVTNNIYITINMDSVLKAKGLLDDNGNPITAGGGRRNGN
                     GGDDGDDSESAVRGRRKKPFRSLGLDDFSDGDYRIDSLDGKPVIRFVVYFEFNEYGLT
                     SHAFGSIDKVISHLKRNGSEFSVEIKGYTDSVGTDQYNNMLSRKRAKMVLDYMNSRGV
                     PTELMKAKAYGSDNPVADNSDPNQAWLNRRAEIIVHRKDVAAQ"
     gene            2437..2670
                     /locus_tag="VKQ52_00535"
     CDS             2437..2670
                     /locus_tag="VKQ52_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85684.1"
                     /translation="MLNLYLNSLWGHLPSNIKIRLIDESGNFIDPLQVKEVDYLSRFI
                     NKCVVNKVYQKMLADRRSGVIEVYNDKVVVKEN"
     gene            complement(2718..3410)
                     /locus_tag="VKQ52_00540"
     CDS             complement(2718..3410)
                     /locus_tag="VKQ52_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ85685.1"
                     /translation="MLRAIAIDDEPIALDVVKALAAKVGFLELVAGYTDAFEAMKRLQ
                     QEKIDLIFLDIKMPDISGIEFLRSIPHPPMVIFTTAYSEHAVQSFELDAIDYLLKPFS
                     QSRFLKACNKAFELYELKRRNGGPAAPEQIFVKSGYGQVRIRLDDILYVQSTGNYMQF
                     VLREEKILSRLTMMEAEALLPSPAFIRIHRSFLVASGKVERIERDTVWIAGRELPVGA
                     GYAREIGKITGT"
     gene            complement(3457..>4061)
                     /locus_tag="VKQ52_00545"
     CDS             complement(3457..>4061)
                     /locus_tag="VKQ52_00545"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ85686.1"
                     /translation="EALGQSEADLQFLRNQINPHFLFNVLNTLYGTALQEDAPRTAGG
                     IQQLGDMMRFMLHENNQDKIPMSKEIEYLRNFIALQQLRTDSSPLIRIETAIDEVLPD
                     GMIAPMLLIPFVENAFKHGISLREPSWVNIRLNGEGKRILFEIRNSVHARQGTDPEKE
                     KSGIGLKNVLHRLNLVYPGRHSFFVNQDEREFFVQLMIEP"
BASE COUNT         1066 a         1281 c          914 g          800 t
ORIGIN      
        1 cccatcggtc gtcatgagac ctacctggcg gcaggcttcc aggccgaata ttatcagagc
       61 cagctcaatg tcgccggcgt aagcggagcc ttcggcgacc agttcgatgc ctatggcccg
      121 atcgaaggcg cacccagcgc cgatcgcctc gccaatggct ggtcctacgg ccacttcaac
      181 ttcaatgccg gtgtctccct cttcagcaat ggccgcaacg acaaatggta tatcggcggt
      241 tcgatgatgc aggttaacca accctacgcc tataagataa aatcggactc cacccgtctc
      301 aaacccgtga tcagcatcca gggcggctat aaatacacga caaaggaaga ggacgaagtc
      361 ggtttctata tgtcgatgag ctgggaaggc cccgcctatc gccactacta cgaactcatc
      421 tatacaaagg ccatccccgg tatcgccggc ggcgccgccg ttggcctcgg cctcggctac
      481 cgatacaatg acgccctcgt tcccaacgtt gaattacgat atcagaaact aacactagga
      541 atcctatacg acgtaaatat ttccaccatc agcgcatccg gtatcgaccg aaacggcatg
      601 gagctggcgc tgaaaatgga cttttaaaaa caatcccatg aagaaggtaa tgattggttt
      661 gattggttgc tctctgctgg cccttacaac ggcccggccg cagaattccg gccggcatgc
      721 cgccggttca caccggcccg ccgcagacac aaccggccag gccaaagagc acacggttat
      781 cacaagggat acgcttcccc cgactaccca ggataccatg tttcggtacc gcggcgacca
      841 tgatacgacc ccggcggtaa aggacaccgt catctccctc gcagatgatg cccccgccgc
      901 tcctgccgtg aaaaaggaca ccgtcaccga tcccccaaaa accgatccgg ccaagacgga
      961 tccgcccaaa acggatgcgg ccaaaacgga tcccaaaccc gccgatcccg cgaatgccga
     1021 tagcaaaaca acacccgccg aacctatcga taccacagcg gaagacaagg ccgaacaaca
     1081 aaaacgcgaa ctcgacagac gctggttcat agccccgctg acaaaactcc agttccagga
     1141 cttcgccatg ctggaaaaga accgcaaagg ctacctcagc gacgctaata ccctgccttt
     1201 cttccagcgc ggtaacgctt cctttgcagc ctcggcttat aagaacctga ccaaccgcct
     1261 ctctatcagc gccgacatcg gcctcagctt cggtcacgca accagcaacg acgtcctgat
     1321 cagccagacc ccatcaaaaa cctataacct gctcaacgca gcgatctatt atcacctgat
     1381 cagcccggcc tatcgcctcc aaccctacgt caccgttggg ttcaacgata tcatcaacga
     1441 caaatcctat cccaccgttc ccctgggcat cggcgccaag ttcaacgccc gcagcatcat
     1501 ggtcctggga caggtatcct atggccagag catctcgaag aatgtagcaa gcacgaccat
     1561 gtacgccgta gggatctata tccccatcaa aagcaaaaaa caaaaacaac tcgacgacga
     1621 cgataactcc cgccttaata agaaagacaa ggacgaagca aagaaaaaag acacggccaa
     1681 caagaacaac aacggcggcg tgaccaataa catctacatc accatcaata tggactccgt
     1741 cctgaaggca aaaggcctcc tcgacgacaa cggcaatccc atcactgccg gcggcggcag
     1801 aaggaacggc aatggcggcg acgacggcga tgatagcgag agcgccgtcc gcggccggcg
     1861 taaaaaaccc ttccgttccc tggggctcga cgacttcagt gatggcgact atcgcatcga
     1921 ctccctcgac ggtaagccgg tcatccggtt cgtcgtctac ttcgagttca acgaatatgg
     1981 cctcacctcc catgctttcg gcagcatcga caaggtgatc agccacctca aacgcaacgg
     2041 ctccgagttc tccgtggaga taaaggggta taccgactcg gttggcaccg atcagtacaa
     2101 taatatgctc tcccggaaaa gagccaaaat ggtgctggat tacatgaaca gccgcggcgt
     2161 ccctaccgaa ctcatgaagg caaaagccta tggtagcgac aaccccgttg cagacaacag
     2221 cgatccaaac caggcctggc tcaaccggcg cgcagagatc atcgtccacc ggaaagacgt
     2281 agccgcccag taacaaggcc ttccagggcc cacaccgccc ggataaccaa cgccgcccga
     2341 taacacatta aaataaaaaa tataacacaa taatttccca gttcgggagt tattgtgtta
     2401 tattgcgaca gtaccaagta aaaatcctta aatccaatgt taaacctgta cttaaactcc
     2461 ctgtggggac atcttccctc caacatcaag atccggctca tcgacgaatc cggcaatttt
     2521 atcgatccgc tccaggtaaa ggaagtcgac tatctttccc gcttcatcaa caaatgcgtt
     2581 gtcaataaag tatatcagaa aatgctggcc gaccgacgtt caggcgttat cgaagtgtat
     2641 aacgacaaag tcgtcgtaaa ggagaactaa taacagccct aaaagagaac tggcaacaaa
     2701 tacggccaaa gagcccctca ggttcccgtt atcttcccga tctcccttgc atagcccgcg
     2761 ccaacaggta gctcccggcc cgcgatccac acggtgtccc gctcgatcct ttctaccttc
     2821 ccgctggcta caagaaagct tctgtgaatt cgtataaaag ccggcgaagg cagcaatgcc
     2881 tctgcctcca tcatcgtcag ccgcgacagg atcttctcct cccgaagcac gaactgcatg
     2941 taattacccg tactctgcac atacaggata tcatccagcc ggatccgcac ctgcccatac
     3001 ccgctcttga caaagatctg ttccggcgcc gccgggcccc cattcctccg cttcagctca
     3061 tatagctcga atgccttgtt acaggctttt aaaaagcggc tttgactaaa tggcttcaac
     3121 aggtaatcta tcgcatccag ctcaaaactc tgcaccgcat gctcgctata ggccgtggta
     3181 aaaatgacca tcggcggatg cgggatgctc cgcaaaaact cgatccccga aatatccggc
     3241 atcttgatat ccaggaagat caggtcgatc ttctcctgct gtaaccgctt catcgcttca
     3301 aaagcatccg tatacccggc caccagctcc aggaacccaa ccttcgccgc cagcgccttc
     3361 accacatcca gcgctatcgg ctcatcgtct atcgctattg ctctcaacat attcgcaaga
     3421 taacgtatgg ttcgatcatc gatttccaaa ggtaacttat ggctcgatca tcaactgtac
     3481 aaagaattcc cgctcgtcct ggttgacaaa aaaggaatgg cgcccgggat agaccaggtt
     3541 gagccggtgc aacacatttt tcaaaccgat acccgacttt tccttctccg ggtcggtacc
     3601 ctgccgcgca tgaacactgt tccggatctc gaacaggatc cgcttcccct ccccattgag
     3661 ccggatattc acccaggaag gttcccgcag cgagatccca tgtttaaagg cattctccac
     3721 gaaggggatc agcagcatcg gggcgatcat cccatcgggc aggacctcat cgatcgccgt
     3781 ttcgatcctg atcagcggcg aggaatccgt cctcagctgc tgcaaggcga taaaattcct
     3841 caggtactcg atttccttac tcattggtat tttgtcttga ttgttttcgt gaagcatgaa
     3901 tcgcatcata tcccccaatt gctggatgcc acccgccgtc cgcggcgcgt cctcctgcag
     3961 ggccgtccca taaagcgtgt tcagcacatt gaaaaggaaa tgcgggttga tctggttgcg
     4021 caggaattgc aggtcggcct cggactgccc cagcgcctcc t
//
LOCUS       DATGFG010000030         8531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_112383, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000030 DATGFG010000000
VERSION     DATGFG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112383"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VKQ52_00550"
     CDS             <1..1324
                     /locus_tag="VKQ52_00550"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HLZ85687.1"
                     /translation="VVTLGSDRHRQDYLIAEGLLQPYEDGSPVSKKMEEMALARIRQL
                     SAHEIGHTLGLTHNFMASVKDRASVMDYPFPRFNLKPDGTIDLSEVYAKGIGGWDKRA
                     ILWGYADFPKGTDEDAALDKIIRETLKEGYKYIPDIGGMTHPLSNQWDDGVNPIDQLD
                     KLMTVRRHLLDNFSEKAIPQNAPMATLEEVLVPIYLLHRYQVEAVAKSVGGLYFTHAV
                     KNDGQEPTKMVDPAEQWRAFDALVNTITPEALILPDDLIRKIPPRPTGYPSTIEIFGG
                     HTGPTFDPTAVAEAAADNTLSYLLNPERAARLIEFQARDEHQPGLLPVLNKLIDKTWK
                     TPLKPGYKGELQLLVDNLALKHLLALAADPKNAENVRGEAMLTITDLSTWMGLQLVNA
                     EPRWKAALYFGLSQIGEFSKNPDKFIPEPALEMPPGAPIGMPDNEWAF"
     gene            1340..2554
                     /locus_tag="VKQ52_00555"
     CDS             1340..2554
                     /locus_tag="VKQ52_00555"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HLZ85688.1"
                     /translation="MRLLIFLWSLLFSCFVRGQSYYLFVGTYTNTGNLAGNPKLDSTG
                     SKGIYVFRFDATTGKATALSHTEGVCNPSYLAIAPGGNTLYACTESRMQKKGSVSAFR
                     FDRAGAQLQFINSVPSGGDNPAYVAVSNSGKWVAVANYTGGSLSVFPVGADGRLGFFA
                     ENIQHTGHGTNPDRQEKPHVHSVLFSPDQKSLYIQDLGLDKIMIEPIDESKDQPVGVG
                     GLPVIVSGTLTPPPGRSAMREPALMVGTMAGVGPRHLTFHPNGKFAYVIEEMGGSVDV
                     YRRNPNTGGLDSLQRIAAHPDTAKGPFRSSDIHVSPDGRFLYASNRAETTITTFAIDP
                     ATGLLRTLGYTLTMGQEPRNFTLDPSGQWLLVANQESDTIVVFRVDPKTGMLRSAGQT
                     LSVPRPTCLKME"
     gene            2831..3616
                     /locus_tag="VKQ52_00560"
     CDS             2831..3616
                     /locus_tag="VKQ52_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HLZ85689.1"
                     /translation="MALPRMIVNADDFGISNTVNRAILESMEIGLVTSTSIMANMPGF
                     EDALTLVHEHPILTGRIGVHLNLTEGHPLSVPILDCPTFCNDNGRFVYDRQRSLFSLT
                     RKGKDAVYEEMRTQLERVLAAGIEPTHLDSHHHLHTEWAIAPLVCRLGREYGIPRVRL
                     ARNMGQPSSLAKRVYKTIFNRWRLGNRRDFRNTDYFGDIEDMKIFLRRHNPGSKSIEI
                     MVHPLFDSEGGLVDLDRQNLYRQLGTILEKQPALCPSPTDPLQ"
     gene            3613..4812
                     /locus_tag="VKQ52_00565"
     CDS             3613..4812
                     /locus_tag="VKQ52_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ85690.1"
                     /translation="MKKLAIVTTHPIQYHVPWLIRLAEKRIAIKVFYTYEQSRNGTVY
                     DPGFGRDIQWDIPLLDGYEYEFVANTSKRPGLENFRGIVNPGLIGKIEAWQPDGLLVI
                     GWNYESHLKCIRHFYNRVPVYFRGDSVLLHERTGLRKLARRIFLTWVYHHVDYALYVG
                     TNNRSYFLRHGLKLSQLIFSPQAIDISRFARPQEDYAPLLRCWKEELGMPGGRLTVLF
                     AGKLTRVKNPGFVLDLAEKCSHLPIDFLLVGDGYLKTELQQRAANMPNVFFMGFQNQS
                     VMPAVYRMGDVYLMPSLSETWGMGINEAMACGIPVMASEQVGCATDLVLENKTGITFH
                     LGDVEKCREFLAALSIDRRRLTEMGACASTLIQFFSFSHIVDSVVRAMTEPVREPGRR
                     SLLNAAL"
     gene            4876..6684
                     /locus_tag="VKQ52_00570"
     CDS             4876..6684
                     /locus_tag="VKQ52_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ85691.1"
                     /translation="MKTFFGHRLINAIAIAIGAVLVIVVLSIWQYRRIRDAGVDLRHT
                     NQVIAQSQRVQGTALQFELNVKNFLLTGDSSFLDTAGHIAPMLSPRIDSLRALIADDP
                     GQRLRIDSLQFYVNGNREVMERAMRLSKGGEFGGAAALINAEARTGYAHRITAVIDQL
                     ETDQHRILDARRAANQDKASLFQWALWSLSAAVALLAVFIFRKMKLDLAGEKMVREEL
                     RENNHLLAEEVRRQTANLRSSDDKYRMLFNKSPLPKWIYDENTMRFLEVNDTAIRAYG
                     YSREEFLAMTIVDIRPPEDVGRLVEDVESIRNQPDAFRNSQWRHIKKNGEVVDVDVTA
                     HAIDFEGRRARMVVVNDITDRRRYEMTLQHLNADLARRATELAASNAELERFAYIASH
                     DLQEPLRMVSSFLQLLHKRYNGQLDAKADQYIHFAVDGAERMKALIMDLLEYSRVGSG
                     KETFGPVDTAVIIKEVGDIFREKIIAARAQVDIGDMPVVVGDKVQITQLFQNLLSNAL
                     KYHSDKPPVIRIGAKEEPGHWLFSVEDNGIGIDAQFFDKIFIIFQRLHNKSDFSGTGI
                     GLAICKKIVERHGGRIWVESLPEKGSTFYFTIHKKG"
     gene            6699..7127
                     /locus_tag="VKQ52_00575"
     CDS             6699..7127
                     /locus_tag="VKQ52_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ85692.1"
                     /translation="MQQIHILLVDDNEGDILLTKEALDDARIINRISIAYDGVDALRF
                     LRKQPKGPDMPDLILLDINLPRMDGTELLGIIKADPDLKRIPVIMLTTSSAEKDILAS
                     YDNYANCYITKPVDLDRFMDVVRTIEDFWISIVKLPKTVQ"
     gene            7128..>8531
                     /locus_tag="VKQ52_00580"
     CDS             7128..>8531
                     /locus_tag="VKQ52_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLZ85693.1"
                     /translation="MEGDIQPIHILVVEDNPGDLFLFKEFLHSTDLPVAEVRHAASLS
                     VAKQLLAIHDIDLLFLDLSLPDSFGLESYTQLQPLTQRVAVILLTGLNDTKIAMQALV
                     MGAQDYLVKGDFDEKLLGRAIRYSLERLRNLQYLRESEERYRELFNNNPMPMWVFDAK
                     TFRFLEANTAAVQHYGYTQEEFLQKTLADIRVDGNRQELLAEVEAIRHNRDGIKKGIL
                     QHVKKDGDIIFVDIAWHSISYKDQPAVLVLANDVTERIKLENELNEQRLNRQKQITEA
                     VILAQEKERTEIGKELHDNVNQILGASNLYINTAMTDEDMRQELLERSTELVSKAINE
                     IRKISKSLITPGLREIGLIESIEDVIDDMKLARADLAIELDLQNISEEQIEDRRKLTF
                     FRIVQEQLNNIVKHARATRVLIRLSMEGENIVVTVADNGVGFDVSRHRKGVGITNIIS
                     RTELFNGKVEIQSQPGEG"
BASE COUNT         1958 a         2543 c         2341 g         1689 t
ORIGIN      
        1 cgtcgtgacc cttggctccg accgccatcg ccaggactat ctcatcgccg aaggcctgtt
       61 gcagccatat gaggatggct ctccggtgtc gaagaagatg gaagaaatgg cccttgcacg
      121 catccgtcag ctgtccgcgc acgagatcgg gcataccctg gggcttaccc ataacttcat
      181 ggccagcgtt aaggaccgcg cttcggtgat ggactatccc ttcccgcgat tcaacctgaa
      241 acccgacggt accatcgatc tttccgaggt ctatgcaaaa ggcatcggcg gctgggacaa
      301 acgcgcgatc ctctggggat acgccgattt cccgaaaggg acggacgagg acgccgccct
      361 cgacaagatc atccgcgaga cgctgaagga aggctataaa tatattcccg acatcggcgg
      421 catgacgcat cccctgtcca accaatggga cgacggcgtg aacccgatcg atcagctgga
      481 taagctgatg accgttcgcc gccacctgct ggataatttc agcgagaaag ccatcccgca
      541 aaatgcgccc atggccacac tggaagaggt gctggtcccc atctacctgc tgcatcgcta
      601 ccaggtggaa gccgtcgcca agtcggtggg cgggttgtat ttcacacatg ccgtgaagaa
      661 tgacggccag gagcccacga aaatggtcga ccccgccgaa caatggcggg cctttgatgc
      721 gctggtcaat accatcaccc ccgaagcgct gatcctgccg gatgacctga tccggaagat
      781 cccgccgcgc ccgacaggat atccatcgac gatagagatc ttcggtggac acaccggacc
      841 cacattcgac ccgacggccg tggccgaggc ggcggcggac aatacgcttt cctacctgct
      901 gaatcccgag cgcgcggcaa ggcttatcga attccaggcc cgggatgaac accagccggg
      961 actgctgccc gtattgaata aattgatcga taaaacctgg aagacaccat tgaaaccggg
     1021 ctataaaggc gaattgcaac tcctcgtcga caacctggcg ctgaaacatc tgcttgccct
     1081 ggccgccgat ccaaagaatg cggagaacgt ccgtggcgag gccatgctga cgataaccga
     1141 tctttccacc tggatgggcc tgcagctggt gaacgccgaa ccccggtgga aggcggcgct
     1201 gtatttcggt ctgtcgcaga tcggggagtt ctcgaagaac cccgacaaat tcatccccga
     1261 gccggcgctg gaaatgccac ccggtgcgcc gatcggcatg ccggacaatg aatgggcgtt
     1321 ttaagataat aacacctcta tgcgtctgct tatcttcctg tggtccttgt tgttctcctg
     1381 cttcgtacgg ggccaatcct actatctttt tgtcggaacg tataccaaca ccggcaacct
     1441 cgcgggtaac cccaaattgg attccaccgg cagcaagggt atctatgtct tccggttcga
     1501 cgctaccacc ggtaaggcca ccgcgctaag ccacaccgaa ggggtttgta acccttccta
     1561 tctggccatc gcccccggcg gtaacacact ctatgcctgc acggagtcgc ggatgcagaa
     1621 aaaggggagc gtcagcgcct ttcgtttcga ccgcgccggc gcgcaactac agtttatcaa
     1681 cagcgttccc agcggcggcg acaatcccgc ctatgtcgcc gtgagcaact cgggcaaatg
     1741 ggtggcggtt gcgaattaca ccggcggcag cctttcggtc tttcccgtcg gtgcggacgg
     1801 ccggttagga ttttttgccg agaacatcca gcatacgggc catgggacga atcccgatcg
     1861 ccaggaaaaa ccccacgtcc attccgtcct cttttccccc gaccagaaat ctttgtatat
     1921 ccaggatctc ggattggata agatcatgat cgagccgatc gatgaaagta aggatcagcc
     1981 ggttggcgtc ggcggattac cggtgatcgt ctccggcacc ctgacaccgc cacccggccg
     2041 gtcggcgatg cgcgaaccgg cgctgatggt gggcacgatg gcaggcgtgg gcccccgtca
     2101 cctgaccttt catccgaatg ggaagttcgc ctatgtcatc gaggaaatgg gtggctccgt
     2161 ggacgtctat cgccgcaatc caaacacggg cgggctggac tcgctgcagc gtatcgcggc
     2221 gcatcccgat acggcaaaag gccccttccg cagttcggac atccatgtct cgcccgacgg
     2281 ccggtttctc tacgcgagca accgggcaga gaccacgatc actacctttg cgatcgaccc
     2341 ggcgaccgga ctcctccgaa cgctggggta tacgttgaca atgggccagg aaccccggaa
     2401 ttttacgctc gacccaagcg gccaatggct gctggtggcg aaccaggaaa gcgacacgat
     2461 cgtggttttc cgggtggatc caaagacggg aatgctccgg tcggcggggc aaaccctttc
     2521 cgtgccaagg cccacatgcc tgaagatgga gtaggcggac cccgcccggc caacagcccg
     2581 cccggccaac agttggagta actggctata aaccgctgat cctgggtgta ttttcaggcc
     2641 cctggggtct taattgcact gaacgataag tgtttatcat tattttttat acacattttg
     2701 ttatagttaa gagataagga cgttattttg ttgttaatag gaagggaacc gcttgcacca
     2761 cttcctctat tgctgacccc cgcaaccggc agacacccct gcttaccctg gccgatctga
     2821 agattttaat atggctttac ccagaatgat cgtgaacgcc gacgacttcg gcatcagcaa
     2881 tacggttaac cgggccatcc ttgagagtat ggaaattggg ctcgtgacaa gcacttcgat
     2941 aatggcgaat atgccgggtt tcgaagatgc ccttactctc gtgcatgaac acccgatcct
     3001 gaccggcagg atcggcgtac acctaaacct taccgagggc cacccattat cggttcccat
     3061 cctggattgc ccaacctttt gcaacgataa cggccgcttt gtctatgacc ggcagcgctc
     3121 gttgttcagc ctgacccgca aggggaaaga cgcggtgtat gaagagatgc gaacccagct
     3181 ggagcgggta ttggctgcgg ggatcgaacc gacgcacctg gattcgcacc accatctcca
     3241 cacggaatgg gcgatcgctc ccctggtgtg ccgcctgggc cgggaatacg gaattccgcg
     3301 cgtccggctc gcccggaaca tggggcagcc ctcaagcctt gccaaacggg tgtacaaaac
     3361 gatcttcaac cggtggcgcc tgggtaaccg gcgcgatttt cgcaatacgg attattttgg
     3421 cgatatcgag gacatgaaga tcttcctcag acgccacaac cccgggagca aaagcatcga
     3481 gatcatggtc catccacttt ttgactccga aggcggattg gtcgacctgg atcgccagaa
     3541 cctctatcgc caactgggga ctatcctgga aaaacaaccg gccctttgtc cttcacccac
     3601 tgaccctcta caatgaaaaa actggccatt gtcactaccc acccgattca ataccatgta
     3661 ccctggctta tccggctggc ggaaaaaagg attgccataa aggtctttta cacctatgag
     3721 cagtcccgca acggcacggt atacgacccc ggttttggca gggacatcca gtgggatatc
     3781 cccctgctcg atggctacga atatgaattc gtcgccaaca cctccaaacg gcccggtctc
     3841 gagaacttcc gcggtatcgt caatcccggc cttatcggga agatcgaggc ctggcagccc
     3901 gatggactgc tggtcatcgg ctggaactac gagagccacc tgaaatgcat ccggcatttt
     3961 tataaccggg tcccggtgta tttccgcggc gattccgtcc tgctgcacga gcgtacggga
     4021 ctgcgtaaac tggcccggcg catcttcctg acctgggtct atcatcatgt ggactacgcc
     4081 ctctatgtag ggaccaacaa tagatcctat tttcttcgtc atggcctgaa gctatcccag
     4141 ctcattttct ccccccaggc catcgacatc agccgtttcg ccaggccaca ggaagactac
     4201 gcacccctgc tccggtgctg gaaggaagaa ctgggtatgc ccgggggccg gctgaccgtg
     4261 cttttcgcgg ggaagctgac cagggtcaag aaccccgggt tcgtcctcga cctcgccgaa
     4321 aagtgcagcc acctccccat cgacttcctg ctggtgggcg atggctacct gaagaccgaa
     4381 ctgcaacaaa gggcggccaa tatgcccaat gtcttcttca tgggtttcca gaaccagagc
     4441 gtcatgcccg cggtctaccg gatgggcgat gtctacctca tgccttccct gagcgagacc
     4501 tggggcatgg ggatcaatga ggccatggcc tgcggcatcc cggtcatggc ttcggaacag
     4561 gtgggttgcg ccaccgatct cgtactcgag aataaaaccg gcatcacctt ccacctggga
     4621 gatgtggaga aatgccggga attcctcgcg gctttgagca tcgaccgtcg ccgactaacg
     4681 gagatgggtg cctgcgcctc gacgcttatc cagttcttct ccttttcgca tattgtcgac
     4741 agtgtggtcc gggccatgac ggaaccggta cgcgaaccgg gtcgccggag tttgttgaac
     4801 gcggcgttat aatacctgtt tttttagtat attacgtaag ttctacgcct gacccgcaac
     4861 caccgcttac caatcatgaa gacattcttt gggcacagac tgatcaatgc catcgctatt
     4921 gcgataggag cggtattagt gatcgtggtg ctttccatct ggcaataccg gcgcatccgg
     4981 gatgcgggtg tcgatctccg gcacaccaac caggtgatcg cccagtccca gcgggtacag
     5041 gggacggccc ttcaattcga actgaatgta aagaacttcc tgctgacggg tgacagcagt
     5101 ttcctcgata cggcgggcca tatcgccccc atgctatccc cacgcatcga tagcctccgg
     5161 gccctcatcg cggatgaccc tggccaacgc cttcggatcg actcgcttca gttttatgtc
     5221 aacggcaatc gggaggtgat ggaacgggcc atgcgtctca gcaagggtgg agaattcggg
     5281 ggcgccgccg ccctgataaa cgccgaagca cggacgggct atgctcaccg gataaccgcc
     5341 gtgatcgacc agctggaaac ggaccaacac cgcatcctcg atgcacgccg ggccgccaac
     5401 caggacaagg cctccctgtt tcagtgggca ttatggtcgc ttagcgcggc cgtggccctg
     5461 ctcgcggttt ttatcttccg gaagatgaag ctcgatctgg ccggggaaaa aatggtccgg
     5521 gaagagctcc gggagaacaa tcacctgctc gcggaggaag tacggcgaca gacggccaac
     5581 ctgcgatcct cggacgacaa atacaggatg ctttttaata agagccccct gcccaaatgg
     5641 atctacgatg agaacacgat gcggttcctg gaggtgaacg atacggctat ccgggcctat
     5701 ggctacagcc gggaggaatt cctggcgatg accattgtgg acatccggcc acccgaggac
     5761 gtcggccgcc tggtggagga cgtggaaagc atccggaacc agcccgacgc cttccggaac
     5821 agtcaatggc gtcatatcaa gaagaacggg gaggtggtcg acgtggatgt gaccgcgcac
     5881 gccatcgact tcgaaggccg gcgggcgcgc atggtggtgg tcaatgatat caccgatcgc
     5941 cgtcggtatg aaatgacctt gcagcacctc aacgccgatc ttgcccggcg ggcgaccgag
     6001 ctggccgcct ctaacgccga actcgaacgg tttgcctaca tcgcctcgca cgacctgcag
     6061 gaacccctcc gcatggtgag cagctttctc caactgctgc acaaaagata caacgggcag
     6121 ctggacgcca aggcggatca atatatccac tttgccgtgg atggcgccga acggatgaag
     6181 gccctcatca tggacctgct ggaatattcg cgggtgggtt cgggcaaaga gaccttcggc
     6241 cccgtggata cggccgtcat catcaaggag gtgggggata tcttccgcga gaagatcatc
     6301 gccgcccgcg cccaggtgga tatcggcgac atgccggtcg tggtggggga caaggtgcag
     6361 atcacccagt tgttccagaa cctgctgagc aacgcattga aataccattc ggacaaaccg
     6421 cccgtgatcc gcatcggggc aaaagaagaa cccgggcact ggctgttctc ggtggaagat
     6481 aacggcatcg gcatcgacgc gcaattcttt gacaagatct ttatcatctt tcagcggttg
     6541 cacaacaagt ccgacttttc gggcaccggc atcggcctgg ccatctgtaa gaagatcgtg
     6601 gaacgacatg gcggccgcat ctgggtcgag tcgttaccgg aaaaaggaag tacgttctat
     6661 ttcactattc ataaaaaagg gtaaccttat aaagttttat gcaacagatt cacattttac
     6721 tggtagatga caacgaaggg gatattctgc tgacaaaaga ggccctcgac gacgcgcgta
     6781 tcatcaacag gatctccatc gcctatgacg gggtggacgc cctccgcttc ctgcgtaaac
     6841 agccgaaggg gccggatatg cccgatctga tcttactcga catcaatctc ccgaggatgg
     6901 acgggacgga attgctgggg atcatcaaag ccgaccccga cctcaaacgt atcccggtga
     6961 tcatgctgac cacctcctcc gccgagaagg atatcctcgc ctcctatgac aattatgcca
     7021 attgttatat cacaaaaccc gtcgacctgg atcgctttat ggacgttgtc cggacgatcg
     7081 aggatttctg gatcagcatt gtcaaacttc caaaaaccgt acaatagatg gagggcgaca
     7141 ttcaaccgat acatatcctc gtggtcgagg acaacccggg agacctgttc cttttcaagg
     7201 aattcctgca tagcaccgat ctgcccgtag cggaggtccg ccacgccgca agcctgtcgg
     7261 tggcaaagca attgctggcg atccacgaca tcgatctcct gttcctcgac ctgtcccttc
     7321 ccgatagttt cggtcttgaa tcctataccc agctgcaacc ccttacccaa cgcgtcgccg
     7381 tgatcctgct caccgggctc aacgatacca agatcgccat gcaggccctc gtcatgggcg
     7441 cccaggacta tctcgtcaaa ggcgatttcg acgagaaact gctcggccgc gccatccgct
     7501 acagcctcga acgtctccgc aacctgcaat acctccgcga aagcgaggaa cgctaccgcg
     7561 agctcttcaa caacaacccc atgccgatgt gggtcttcga tgcaaagaca ttccgtttcc
     7621 tcgaggcgaa cacggcagcg gtacaacact acggctatac ccaggaagag ttcctgcaaa
     7681 agaccctggc cgatatccgc gttgacggca accgtcagga gctgctcgcc gaagtggaag
     7741 ccatccggca caatcgcgac ggcatcaaaa aaggcatcct gcaacacgta aagaaggatg
     7801 gagatatcat ctttgtcgat atagcctggc atagcatcag ctataaggac cagccggctg
     7861 tcctggtgct ggccaacgat gtaacggaaa ggatcaagct cgagaacgaa ctcaacgaac
     7921 aacgcctcaa ccgccagaag cagatcacgg aggccgtgat cctcgcgcag gagaaggagc
     7981 ggacggagat cgggaaggaa ctgcacgata acgtcaacca gatcctcggc gcgagcaatc
     8041 tctatatcaa caccgcgatg accgatgagg acatgcgcca ggagctgctg gaacgcagta
     8101 ccgaactggt aagcaaggcc atcaacgaga tcaggaagat ctccaaatcc ctcatcactc
     8161 ccggactgcg ggagatcggg ctgatcgagt ccatcgagga tgtcatcgat gacatgaagc
     8221 tcgccagggc cgatctggcg atcgagctcg acctgcagaa catctccgag gaacagatcg
     8281 aagacaggcg gaaactcacc ttctttcgca tcgtccagga acagttgaat aatatcgtaa
     8341 aacacgcccg cgccacccgc gtcctgatcc gcctctcgat ggaaggcgag aatatcgtcg
     8401 tcaccgtcgc ggacaacggg gtcggcttcg acgtcagccg gcaccggaaa ggagtgggca
     8461 tcactaatat catcagccgg acggagctct tcaacggcaa ggtcgagatc cagtcccaac
     8521 ccggcgaagg g
//
LOCUS       DATGFG010000031         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1479179, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000031 DATGFG010000000
VERSION     DATGFG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479179"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..821
                     /locus_tag="VKQ52_00585"
     CDS             219..821
                     /locus_tag="VKQ52_00585"
                     /inference="COORDINATES: protein motif:HMM:NF005754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HLZ85694.1"
                     /translation="MRILIIGGQGTIGKKVAATLSSRHDIIIAGRNSGDARVDIGSAA
                     SIGELFRKLHTIDACICTAGTGYYGEFDTMTEEHMQSGIRGKLLGQVNTVLIGKDYLT
                     EGGSFTLTSGIASEEPARNGTTVAMINGAINSFVLGAAQELKKGKRINAVSPGLVEDS
                     MERYGHLFPGYNAVPMARVVNGYLLSVEGWVNGRIIKVYS"
     gene            964..1584
                     /locus_tag="VKQ52_00590"
     CDS             964..1584
                     /locus_tag="VKQ52_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HLZ85695.1"
                     /translation="MQITAKHQLYRLSVLAAVAVLTSVACNNSDYKTSDNSSQPDSIV
                     GGANRSTTKDVAAQDSNAVQMAPRTSPATAVGPATAKTAPAKVAKRRFTSVTTPAAMA
                     AGGSSAPQFPGGQKALDNYVNDHVNYPQDAVDNDVSGVVRVSFVVDEKGRITKAKLIG
                     AQKVGDGLDEEALRVVSTMPAWKPATVHGKKVKTRLELPISFQVES"
     gene            1653..>2214
                     /locus_tag="VKQ52_00595"
     CDS             1653..>2214
                     /locus_tag="VKQ52_00595"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HLZ85696.1"
                     /translation="MTVAAIMAELKSLGDPQIKSILQKHGVREPLFGVRVADLKPIQK
                     KIKKDYQLAKDLYATGNADAMYLAGLIADDPKMTANDLRSWVRQALSQNISEYTVPWV
                     AAGSPQGYTLALEWIDAKEEHIAAAGWATLGAWVSLKPDGELDIPALKALLSRVAKAI
                     HTAPNRVRYTMNSFIVGVGAYVTPLTK"
BASE COUNT          528 a          656 c          612 g          418 t
ORIGIN      
        1 cgcctcgccc atgcgcaatt gctgttgcgg caaacaagcc agaaggtgtc ggacatcgcc
       61 atctcctgcg gcttcgaaag cgtctcccat ttcatccgca tcttccggaa agagtttggc
      121 gtcacccccc agtccctgcg agccgaaaaa gcgatgagtt gagctttttg cattacggcc
      181 tcccctgccg gcagggctag ctttgcaaaa aaagaatcat gagaattttg atcatcggtg
      241 gtcagggaac catcggaaaa aaagtggccg caacgctctc ttcacgccat gacatcatca
      301 tcgccggccg caatagcggc gacgcccggg tcgacatcgg ttccgccgca tccatcggcg
      361 agctgttccg gaaactgcat accatcgatg cctgcatctg caccgccggt acgggatact
      421 atggagaatt cgacaccatg accgaagagc atatgcaaag cggcatccgg ggaaaattgc
      481 tggggcaggt gaataccgtc ctcatcggta aggactatct caccgaaggc ggctccttta
      541 ccctcacctc cggcatcgcc tcggaagaac ccgcgcgcaa cggaacgacc gtagccatga
      601 tcaatggcgc gatcaacagc tttgtgctcg gcgcggcgca ggaattaaaa aagggaaaac
      661 gcatcaacgc ggtgagcccc ggcctcgtgg aagattccat ggagcgctat ggccacttgt
      721 tccccggcta taacgccgtg ccgatggcaa gggtcgtcaa tggctacctg ctgagtgtcg
      781 aaggctgggt gaatggcagg atcatcaagg tctattccta agccaggtcc taggcctggg
      841 cctggggaag aacttcccca ccccgccggg gcgctcttcc catcctgctg caggcgccct
      901 tttccccccg gctggcatcg tctttgaatc accatcggta tatgttcacc aaaaaaagga
      961 tttatgcaaa ttacagcaaa acaccaactt taccggttaa gtgtactcgc tgcggttgcc
     1021 gtactcacct cggtggcctg taataacagc gattataaga caagcgacaa ttccagccag
     1081 cccgatagca ttgtcggcgg cgccaataga tcgacgacaa aagatgtagc cgcacaggac
     1141 agcaatgccg tgcagatggc gccccggacc tcgcccgcga cggccgtagg accggccacg
     1201 gcgaaaaccg ccccggcgaa ggtcgcaaag agaaggttca cctccgtaac gacgcccgcg
     1261 gcgatggctg caggcggaag ctcggctccc caattccccg gcggccagaa agcgctcgat
     1321 aactatgtca acgatcatgt caactatccg caggacgcgg tggataacga cgtatccggc
     1381 gtggtacgtg tctcgttcgt agtggatgaa aaaggccgga tcacaaaggc aaaactgatt
     1441 ggcgcacaaa aggtaggcga tggtctcgac gaagaagccc tgcgggtggt cagcaccatg
     1501 cccgcctgga aacccgcgac cgtacatgga aagaaggtaa agactcgcct tgagttaccc
     1561 atctctttcc aagtggaaag ctgatgggct ttatcacctg gacacacacg aaggcctcca
     1621 cagttgtgga ggcttttttg tatcttcatc ctatgaccgt tgccgccatc atggccgaac
     1681 tcaagtcgct gggcgacccg cagataaaaa gcatcctgca aaagcacggt gtccgggaac
     1741 ccctattcgg tgtccgcgtg gccgatctga aaccgattca gaaaaagatc aaaaaggatt
     1801 atcagttggc caaggacctc tatgctacgg gcaatgcgga cgccatgtac ctggccgggc
     1861 tgatcgccga cgacccaaag atgacggcca atgatctgcg atcctgggtc cggcaggccc
     1921 tctctcaaaa tataagcgaa tataccgttc cgtgggtagc cgccggcagc ccgcagggct
     1981 ataccctcgc gctcgaatgg atcgatgcaa aggaagaaca tatcgccgcc gccggctggg
     2041 cgacactggg tgcctgggtc tcgctgaagc cggatggcga actggatatc ccggcattga
     2101 aggccctgtt atcccgcgtg gcaaaggcca tccacaccgc ccccaaccgc gtgcggtata
     2161 ccatgaatag ctttattgtc ggtgtaggcg cctatgtgac gccgctgacg aagg
//
LOCUS       DATGFG010000032         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_793563, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000032 DATGFG010000000
VERSION     DATGFG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793563"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..976
                     /locus_tag="VKQ52_00600"
     CDS             131..976
                     /locus_tag="VKQ52_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLZ85697.1"
                     /translation="MIARRKFLQTAGLGVVGLGMPALPALAGPAGKAGPSAMPLKMGI
                     AGYTFLHVPPEQGIAMMKRVGVEALSIKDFYLPLDSNEEVIQGVVGKFAAAGIRIYAA
                     GVIYMKTQAEVDRAFDYAKKLGTDLIIGVPNPELLGYTELKVKAYNIRVAIHNHGPED
                     KLYPSPVDVYDHIKGLDQRIGLCLDIGHAARAGADIVKVVADYGPRILDMHIKDLSVI
                     ARESRPIELGRGVLDIVGLVKALIRMRYAGYCSIEHEMDMNDPLPGIAESAGYFRGVQ
                     ATASH"
     gene            1226..2296
                     /locus_tag="VKQ52_00605"
     CDS             1226..2296
                     /locus_tag="VKQ52_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HLZ85698.1"
                     /translation="MIIDKNDLRDFQQVNLVANKWTYGPKTVDTTLQNAWGLTWSPTG
                     IAWVNSNGGHVSELYTAEGGIVRPGVRIPSPTDTMGGVPSGVVFSGGKGFALANDKQG
                     SLFLFVGEDGVLSGWNGAEGNNALRIKDRSATSIYKGLALASSGGANYIYATNFKTGK
                     IDVWDTTFAPVSMPFNDPGIPAGYAPFNIQAIGSWLFVTFAHQKPGSGDEMDGPGLGF
                     VDVFNTDGSFVRRFASHGPLNAPWGVTMAPAGFLEENDMSADNGKGNSGSNSNDHSKK
                     DVSVPVILVGNFGDGHINVFAEDGEFLGHLRSHSQEIVIDGLWALSFPPATATAIDPK
                     RLYFTAGPNDENDGLFGYLIKQ"
     gene            complement(2305..>2571)
                     /locus_tag="VKQ52_00610"
     CDS             complement(2305..>2571)
                     /locus_tag="VKQ52_00610"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HLZ85699.1"
                     /translation="AVLFWFLKKRDIFPRMFLWYIGILLSGRLMLLLLFTFIPIPAAL
                     DTYKYELTAHFIRTILYAAICVTYILQSGQVKSTFLEPFRERLR"
BASE COUNT          534 a          572 c          848 g          617 t
ORIGIN      
        1 gcagccgcat ggaggtggtg aacagccgca tggaggcggt ggaggtggag aacatcatca
       61 ccggtgagcg gctttttgtt tggtttaaat cagtatattc gaagggcccg tttttgcggg
      121 cccttttttt atgatagcga gaagaaaatt cttacagaca gccggcctgg gtgtggtcgg
      181 attggggatg ccggcgttgc cggctttggc ggggcctgcg gggaaggcgg ggccgtccgc
      241 gatgccgcta aagatgggga tcgcagggta tacctttttg catgttccgc cggaacaggg
      301 gattgcgatg atgaagcggg tgggggtcga ggcgttgtcc atcaaggatt tttatttgcc
      361 gctggatagc aatgaggagg tgatacaggg ggtagtgggt aaattcgccg cggcgggtat
      421 ccgcatctat gccgccgggg ttatttatat gaagacccag gccgaagtcg accgggcttt
      481 tgactatgcg aagaagttgg ggaccgacct catcatcggg gtgcccaacc cggaattact
      541 gggatatacg gagctgaagg taaaggccta taatatccgg gtggccattc acaaccatgg
      601 tcctgaggat aagctttatc cgtcgccggt ggatgtgtat gatcatatca aggggctcga
      661 ccagcgtatc gggctttgcc tggatatcgg gcatgcggcc cgggcgggcg ccgatatcgt
      721 gaaggtggtg gcggattatg ggccgcggat cctcgatatg catatcaagg acctctcggt
      781 catcgccagg gagtcgcgtc ccatcgagct tgggcgtggg gtgctggaca tcgttggact
      841 tgtaaaagca ttgatacgga tgcgatatgc gggctactgc agtatcgaac acgagatgga
      901 tatgaacgat cccctgccgg ggatcgcgga gtctgccggg tatttcagag gagtacaggc
      961 aaccgcctct cactaggctc cgtttgttct tcatacctgc tgaaacctgt gcgcacagga
     1021 gagctcccct tttccctttt tcttcttttt taaccagcga ttgcgttcgg caagcgctga
     1081 acggtcgcct atttatcaac tcactctaac tttaaaattg ctgattatga acacctttca
     1141 acgattacca gtacgactgc gcgttttttc gagtcgttgg ccgctgttgg tttgttgtgc
     1201 gctggcggtc gcttcctgcc ataaaatgat tatcgataaa aatgacctgc gggattttca
     1261 gcaggtgaat cttgtcgcca ataaatggac ctatggtccg aagacggtgg acacaacgct
     1321 gcagaatgcg tgggggctga cgtggtcgcc gaccggtatt gcatgggtca attcgaatgg
     1381 cggtcatgtc agtgaattgt acactgccga gggggggatc gtacgtccgg gggtgcggat
     1441 cccttcaccc accgatacga tgggcggggt gcccagcggg gtcgttttca gcggcggtaa
     1501 gggttttgcc cttgccaacg acaagcaggg ctcgcttttc ctgtttgtgg gggaggatgg
     1561 cgttctatcg ggctggaatg gggcggaggg caacaatgcc ctgcggatca aggaccggtc
     1621 ggcgacctcc atctataagg ggctggcact ggcgtccagt ggcggagcca attatatcta
     1681 tgccactaat ttcaagaccg gcaagatcga tgtgtgggat accacttttg ctcccgtgtc
     1741 gatgcctttt aatgatccgg gtattccggc ggggtatgcg ccgttcaata tccaggcgat
     1801 cggcagttgg ctgtttgtga cctttgccca tcagaaaccg ggcagtgggg atgaaatgga
     1861 tggcccgggc ctggggtttg tggatgtctt caatacggat ggttcctttg tcaggcgctt
     1921 tgcgtctcat gggccgctga atgccccctg gggtgtgacg atggcgccgg caggtttcct
     1981 ggaagagaat gatatgagtg cggacaatgg aaaagggaat agcgggagca acagtaatga
     2041 ccattctaag aaagatgtca gtgtgccggt gatcctcgtg gggaatttcg gggatgggca
     2101 tatcaatgta tttgccgagg atggtgaatt cctggggcat ctgcgtagtc atagccagga
     2161 gatcgttatc gacgggctct gggccttgtc tttcccgcct gccacggcga cggcgatcga
     2221 tcccaagcgg ctatatttta ccgcgggtcc caatgatgag aatgatggtc tttttggata
     2281 cctgatcaaa caatagaaca ggcgttagcg gaggcgttcg cgaaacggtt cgaggaaggt
     2341 gctttttacc tggccggatt ggaggatata ggtcacgcag atggcggcgt agaggatggt
     2401 gcggatgaag tgggcggtga gttcgtattt ataggtatcg agtgcggccg ggatggggat
     2461 gaaggtaaag agcaggagga gcatgaggcg gccggagagc aggatgccga tgtaccagag
     2521 gaacatccgg gggaagatat cccttttctt caggaaccag aagaggacgg c
//
LOCUS       DATGFG010000033         8362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1364388, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000033 DATGFG010000000
VERSION     DATGFG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364388"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VKQ52_00615"
     CDS             <1..412
                     /locus_tag="VKQ52_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLZ85700.1"
                     /translation="IKFFINSPGGVVTSGFIIYDTMKMLKSPVSTICMGLAASMGSIL
                     LSGGEKGRRFVYPLSEIMIHQPSLGGYMQGVSVDLEITAKQTRRVKDISARILAENCG
                     KKFDQVMKDFDRDYWMDAKEAMEYGIVDHIFEKS"
     gene            512..1747
                     /gene="clpX"
                     /locus_tag="VKQ52_00620"
     CDS             512..1747
                     /gene="clpX"
                     /locus_tag="VKQ52_00620"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HLZ85701.1"
                     /translation="MAKNTLHCSFCGRNRDEVKILIAGQEGHICENCVEHAQEIIQQE
                     LMIREDSAGSSSFKLTVKKPIEIKRILDEYVIGQDDAKKVLSVAVYNHYKRLQQKVVE
                     NEVEIEKSNIIMVGETGTGKTLLAKTIARLLNVPFAIVDATVFTEAGYVGEDVESILT
                     RLLQVCNYDVAAAERGIVYIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGTDVL
                     VPPQGGRKHPEQKLIKINTQNILFVCGGAFDGVDKIIARRINTNAIGFNVNKEQQEAA
                     KKNLLQYVNAPDLKSFGLIPELLGRLPVVTHLDPLDKPTLRSILTEPKNSLIRQYKKL
                     FELEGIQLSFDIEVLDFMVDKAVEYKLGARGLRSICESILTDAMFELPSAKEDKFHLD
                     LEYAQKKFDKSKMNLLKVA"
     gene            1751..1924
                     /locus_tag="VKQ52_00625"
     CDS             1751..1924
                     /locus_tag="VKQ52_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85702.1"
                     /translation="MQELIARLMAEGLTEDQAFKAVEIIKNFTKEKFPLFSGAIDKLF
                     DKYGPKTEEDFMP"
     gene            complement(1993..3135)
                     /locus_tag="VKQ52_00630"
     CDS             complement(1993..3135)
                     /locus_tag="VKQ52_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLZ85703.1"
                     /translation="MEFATDELTQQVGRTARDFANQYIRPYVTEWDENQTFPHEVFKE
                     LGRLGMMGVVVPEEYGGSGLSYLEYVTIIREISAVCGAVGLSLAAHNSLCTGHILAFG
                     NEHQKKKYLPLLSTGQALGAWGLTEANTGSDAGNMACTAVKEGDEWVINGTKNWITHG
                     ISGDIAVVVTRTGEPRTRNNATAFIVERGTPGFSGGKKENKLGMRASETAEMIFDHCR
                     VPDANRMGEVGDGFRQALAVLDGGRISIAALSLGIAKGAYEAALQYSKERHQFDQPIA
                     NFQGISFKLADMATELSAAELLTFQAADRKNKHLPVTREAAMAKYYASEVAVRVATDA
                     VQIFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKLVIAREVLKA"
     gene            3258..3488
                     /locus_tag="VKQ52_00635"
     CDS             3258..3488
                     /locus_tag="VKQ52_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85704.1"
                     /translation="MQDTFFFSQILTDVSLLKKWLKKGPAKGGPEGRRPKGRYKTQAP
                     RKAENESQMKGGKIEKRPRLRGAPEVGINHNI"
     gene            complement(3515..4210)
                     /locus_tag="VKQ52_00640"
     CDS             complement(3515..4210)
                     /locus_tag="VKQ52_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85705.1"
                     /translation="MRKIILILPGLLFLSAGVFAQTSVELIPTAGYTFANRTDFYNTY
                     GRISDGFSYGGSIKFNVNRSFGIEVLYSHMNTTTGLYYYGPDQTPVGQYNLKFDYIMA
                     GPIESFTIPNSTVRPFIGALLGAAIMTPDMSSGYSTDTRFAVGFQLGTNIYLSPRVGI
                     QLKAQLLSPVDGAGGGFFVSNYGGGGGIDTYSSIYQFSLSGGIIIGLGRVLPAQVYHP
                     ARRPRPRYYRYYY"
     gene            complement(4267..5043)
                     /locus_tag="VKQ52_00645"
     CDS             complement(4267..5043)
                     /locus_tag="VKQ52_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLZ85706.1"
                     /translation="MKNPPLKITFLGTGTSTGVPMVACPCEVCASTDARDKRLRSSIL
                     VQSPTTTIVVDTTPDFRFQMLREEIRRLDAVIFTHPHKDHLAGLDDVRAYNFFQHKAM
                     DLFANEMTQESIVREFPYAFADMKYPGIPDLRLIRIDKDPFTVGDITVIPIQVWHMKM
                     PVLGFRFGNFTYITDANRIDETEREKIRGSEQIVLNALRHEKHLSHFSLTEAIEEVRQ
                     LGVPRAWFTHVSHQLGRYADIAPTLPPGMQLAYDGLVLHA"
     gene            complement(5119..5400)
                     /locus_tag="VKQ52_00650"
     CDS             complement(5119..5400)
                     /locus_tag="VKQ52_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85707.1"
                     /translation="MMKYTQSTLDKIERILDETEYIVRYERGNFQSGYCILEQRKVVV
                     LNKFLQLEGRISTLIDIVPQLKINPDDLSPEVRKIYDDVVATAHQISES"
     gene            complement(5499..7061)
                     /locus_tag="VKQ52_00655"
     CDS             complement(5499..7061)
                     /locus_tag="VKQ52_00655"
                     /inference="COORDINATES: protein motif:HMM:NF020249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HLZ85708.1"
                     /translation="MPLAKILWVDDEIESLHSQILFLENKGYEVKALTNGFDAIDFVK
                     DNPVDVVLLDETMPGITGLETLAKIKEANQQIPVVLITKNETENLMDDAIGSQITDYL
                     IKPVNPNQVLLSLKKIIDNKRLVSEKTTTAYQQQFRNLFMALNSNPDYNEWMDIYKKL
                     IYWELEMEKSDSPEMREVLQSQKQEANTEFFKFLSKNYASWVHPRSSEGPIMSHSLFQ
                     FKVLPHVEKGTPTFFLLLDNLRIDQWKAIQPIFAESFRILEEDSFYSILPTATQYSRN
                     AIFSGLMPVDIEKQFPSQWKNDDEEGGKNLYEEEFFRAQLKRLKKDHLRFSYTKVLNY
                     QAGQDLVANIHNLLNNDLNIIVYNFVDMLSHARTEMEVLKELASDEISYRSITASWFE
                     HSPLHSALRKIADKKINLILATDHGSVRVKTPYKVIGDKQTTTNLRYKHGRNLNYEAK
                     EVLAFRDPKEAGLPVPTVNSSFIFARGDGFLCYPNNYNYYVNYYRNTFQHGGVSLEEM
                     IIPVIRMTSKGA"
     gene            7145..>8362
                     /locus_tag="VKQ52_00660"
     CDS             7145..>8362
                     /locus_tag="VKQ52_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLZ85709.1"
                     /translation="MAQSSRKIINDPVHGFITLDHPVLLNIVAHPYYQRLRRINQMAF
                     AHLVYPGAVHTRLHHSLGAYHLMGCAVTELQRKGFPISEEEALGVKIAILLHDIGHGP
                     FSHALENVLIRGVHHETLSLLIMKVLNEDLDGQLEMGINIFTGGYPKRFLHQLVSGQL
                     DMDRMDYLTRDSFFTGVSEGVIGYDRILKMLMVHKGELMVEEKAVYSIEKFLVARRLM
                     YWQVYLHKTVVSAEKMLVRILERARELIVMGTQVPSSSPVLDFFLQDRAEVLDAGANL
                     DKFCLLDDYDVMCTIKLWMFHSDTVLSLLSRSLIDRELLKVRFQATPFDENYVKELRE
                     RVATGLGIAREEARYFVFTGEAVNTTYNSMDEKINILFRDASVRDISEVDNALIQQTL
                     ASTVKKFYICHLNT"
BASE COUNT         1967 a         2095 c         2356 g         1944 t
ORIGIN      
        1 gatcaaattc ttcatcaaca gccccggcgg ggtcgttacc tccggcttta tcatctacga
       61 caccatgaag atgctgaaga gccccgtgag cacgatctgc atgggtctgg cagcctcgat
      121 ggggtcgatc ctgctcagtg gtggggaaaa aggccgtcgt tttgtctatc ccctgtccga
      181 gatcatgatc caccagccca gccttggcgg atacatgcag ggagtgagtg tagacctgga
      241 aatcacggca aaacaaaccc ggagagtaaa ggatatcagc gcccggatcc tggccgaaaa
      301 ttgcggcaag aagtttgacc aggtaatgaa ggatttcgac cgggattact ggatggatgc
      361 aaaagaggcg atggaatatg gcatcgtgga ccatattttt gagaagtcgt agcacaagtc
      421 gctgattagc aatggcctct tttttccacg ccactgtgag aatccaataa gttgggtaac
      481 tttgtagttc aaacctggat aattagctat aatggcaaaa aatacactgc attgttcttt
      541 ctgcgggcga aatagagacg aggtaaagat ccttattgcc gggcaggaag ggcatatttg
      601 cgaaaattgt gtggaacatg ctcaggagat cattcagcag gagctgatga tccgcgagga
      661 ttcggccggc agcagttcgt tcaaattaac cgtcaaaaag cctatcgaaa taaagcgtat
      721 cctggacgag tatgtcatcg gccaggacga tgcgaagaag gtattgtccg ttgccgttta
      781 caatcactac aagcgtctcc agcaaaaagt ggtggagaac gaggtggaga tcgaaaagag
      841 caatatcatc atggtcggtg agaccggtac cgggaagacc cttctcgcca agacgatcgc
      901 ccgcctgctg aatgttccct tcgccatcgt ggacgccacc gtctttaccg aagccggtta
      961 tgtcggggag gacgtggaaa gcatcctgac ccgcctctta caagtctgca actacgatgt
     1021 cgccgccgcc gaacggggga tcgtgtacat cgatgagatc gataagatcg cccggaaagg
     1081 cgacaatccc tccatcaccc gcgacgtcag cggggaaggc gtacagcagg gtctgctcaa
     1141 gctgctggaa ggtacggatg tacttgttcc gccccagggt ggccggaaac atcccgagca
     1201 gaagctgatc aagatcaata cgcaaaatat cctcttcgtc tgtggcggag cctttgacgg
     1261 cgtggacaag atcattgccc gccggatcaa taccaacgcc atcggcttta acgtcaataa
     1321 ggagcagcag gaagccgcca agaagaacct gcttcagtac gtgaatgcgc ccgacctgaa
     1381 atcgtttggg ttgatccccg agctgctggg ccggctgccc gtcgttaccc acctcgatcc
     1441 gctggacaag ccgacgctgc gttccatcct caccgaacca aagaactcgc tgatccgcca
     1501 gtacaagaaa ctgttcgaac tggaaggcat ccaattgagc ttcgacatcg aagtgctcga
     1561 tttcatggtg gacaaagccg tggaatacaa attgggcgcc cgtggactgc ggagcatctg
     1621 cgaaagcatt ctcaccgacg cgatgttcga actgccctcg gcaaaggagg acaaattcca
     1681 tctcgacctc gagtatgcgc agaagaagtt cgacaagagc aaaatgaatc tcctaaaagt
     1741 tgcctaacat atgcaggaat tgatcgcgcg gctgatggcc gaagggttga ccgaggacca
     1801 ggcctttaaa gccgtcgaaa tcatcaagaa cttcacaaaa gaaaaattcc ccctcttctc
     1861 aggagcgatc gacaaacttt tcgacaaata cgggccaaag acggaagaag acttcatgcc
     1921 atagcgccgg aggcgccttt ctgccttccc cagccggccg ggaggccggc ttcgcggagc
     1981 ccgcgcaaca gattacgctt tcaacacctc gcgggcgatg accagtttct ggatctcgct
     2041 ggtgccttcg ccgatggtgc agagtttgct gtcccggtag aatttctcca ccgggaaatc
     2101 cttggtatat ccatagccgc cgaagatctg gacggcatcg gtggcgacgc gcaccgctac
     2161 ttcgctggcg taatacttgg ccatggcggc ctccctggtc accggcaggt gcttgttctt
     2221 ccggtcggcg gcctggaagg tcaggagctc ggccgcggat agttcagtgg ccatatcggc
     2281 taatttgaag gagatgccct ggaaattggc gatcggctgg tcgaactggt ggcgttcttt
     2341 actatactgc agagcggctt cgtatgcgcc ttttgcaatg cccagcgaga gcgcggcgat
     2401 ggagatgcgc ccgccatcca gcacggcgag ggcctggcga aagccatcgc ccacttcccc
     2461 catacggttg gcatcgggaa cccggcagtg atcgaagatc atctcggccg tttcactggc
     2521 gcgcatgccc agtttattct cttttttccc gccgctgaat ccgggtgttc cccgttcgac
     2581 gataaaggcg gtggcattat tccgggtgcg gggttcgccg gtacgggtca caacgacggc
     2641 aatatcgccg ctgatgccat gggttatcca gttctttgtt ccgttgatca cccattcatc
     2701 cccttctttt acggccgtac aggccatatt gccggcatca ctgccggtat tggcttcggt
     2761 gagtccccag gcgccaaggg cctggccggt ggaaagcagg ggcaggtatt tctttttctg
     2821 gtgttcgttg ccaaaggcaa ggatatgccc ggtgcagagg gaattgtggg ccgcgaggct
     2881 aagcccgacg gcgccgcaga ccgcggaaat ctcccggatg atggttacgt attcgaggta
     2941 ggacaggccg gaaccgccat attcttcggg aacgacgacc cccatcatgc cgaggcggcc
     3001 taactcctta aaaacctcgt gcgggaaggt ctggttctcg tcccattccg ttacataggg
     3061 tctgatatac tggttagcaa aatcccgggc cgtacggccc acttgttggg taagttcatc
     3121 tgttgcaaat tccatctaac atgctttgtg ttcgcgcttt gcgttaaaag aacccggcaa
     3181 caaccttaca ggttattgcc gggaaaacac tggcaaacaa tcattatcgg taaaagcgtc
     3241 tcaaaagact aatctacatg caagatacat tctttttttc acaaatacta acagatgtta
     3301 gtttattgaa aaaatggcta aaaaaaggcc ccgctaaggg cgggccggag ggccgccgcc
     3361 cgaagggcag atataagacg caagccccgc gcaaagcgga aaatgaaagc cagatgaaag
     3421 ggggaaaaat agaaaaaagg ccccgcttgc gcggggcccc tgaggtaggg atcaaccaca
     3481 atatatgaga tcaagggtac cgtatgtcgg ttgtttaata ataataccgg taataccggg
     3541 gtctgggcct ccgggcggga tgatacacct gcgcggggag tacgcggccg agaccgataa
     3601 tgatcccgcc gctgagggag aactggtaga tcgacgagta ggtgtcgata ccgccgcccc
     3661 caccatagtt gctgacgaag aaaccgccgc cggcgccatc cacgggggac aggagctgcg
     3721 ccttcagctg gatgccgacc ctgggtgaaa ggtaaatatt cgttccgagc tggaagccca
     3781 ccgcaaagcg cgtatcggtg gaataacccg agctcatatc cggtgtcatg atggctgcac
     3841 caagcagggc gccgataaac ggccggacgg tggagttggg gatcgtgaag gattcgatgg
     3901 gaccggccat gatgtaatcg aacttaagat tgtattggcc caccggggtc tggtcgggtc
     3961 cgtaataata caggccggtg gtcgtattca tatgactata aagcacttcg atcccaaagc
     4021 tacggttgac attgaatttt atgctcccgc catagctgaa gccgtccgag atccggccat
     4081 atgtgttgta aaaatctgtc cggttggcaa aggtgtatcc ggcggtgggg atcagctcaa
     4141 cggaggtttg cgcaaacact ccggcggaca ggaagagcag cccgggcagg atcaggatta
     4201 tttttctcat acttccgatt ttatgaaggc tatagacaca agatcggccg caaggtttaa
     4261 tcgcgctcat gcatgtaaca ctaatccatc ataagccagt tgcattcccg gcggaagggt
     4321 aggcgcgatg tcggcatacc tgcccaactg atggctgaca tgggtgaacc aggcccgtgg
     4381 gacacccagc tgccgaactt cctcgatcgc ttcggtaagc gagaaatgcg aaagatgttt
     4441 ttcatgccgc agcgcgttga gaacgatctg ttcgctgccc ctgatctttt cgcgttcggt
     4501 ctcgtcgatg cggttggcgt cggtgatata ggtgaagttg ccaaaacgaa aaccgaggac
     4561 gggcatcttc atatgccaga cctggatcgg gatcaccgtg atatcgccga cggtgaacgg
     4621 gtccttgtcg atgcggatga ggcggagatc gggaatgccg ggatacttca tgtcggcgaa
     4681 agcatagggg aattccctta cgatggactc ctgggtcatc tcgttggcaa agaggtccat
     4741 cgccttatgc tgaaaaaaat tataggcccg gacatcatcc aggccggcca gatgatcctt
     4801 atggggatgg gtgaagatga cggcgtccag ccggcggatc tcttcccgca gcatctggaa
     4861 ccggaaatcc ggcgtggtat cgacgacgat ggtggtggtg ggagactgga caaggatgct
     4921 ggagcggagt ctcttgtccc tggcgtcggt ggaggcacat acttcacagg gacatgctac
     4981 catgggaacg cccgtacttg tgcccgtacc caggaaagtg atttttaatg gcgggttctt
     5041 cacgggggta aatttactaa atcaacggag aaaagcactt actaaatcga cggggaaaag
     5101 tatccgtaaa aaacaggcct agctctcgct gatctggtgg gccgtggcga ccacatcatc
     5161 atagatcttg cggacctcgg ggctgaggtc gtccgggttt atctttagtt gtgggacaat
     5221 atcgatcagc gtgctgatcc ggccctccaa ctgaaggaat ttattcagca ccactacttt
     5281 cctctgttcg aggatacagt acccgctctg gaaattaccc cgctcatagc gaacgatgta
     5341 ttccgtctcg tccaggatgc gctcgatctt atcgagcgta gattgtgtgt acttcatcat
     5401 agtatgcagg gataaaggta ctacaatacg gcgggagggg ggaatcggcc tattcgtttt
     5461 tatccacagg aaccggagtg atgtggaaag aataacattt aagccccctt actggtcatc
     5521 cggatcacgg gaatgatcat ttcctccagg ctgacgccgc catgctggaa agtattccgg
     5581 taatagttga catagtagtt atagttattg gggtagcaaa ggaacccatc gccccgggcg
     5641 aagataaagg aggaattgac cgtcggcacg ggcagaccgg cctccttcgg atcgcggaaa
     5701 gcaaggactt ccttggcctc ataattcaaa ttacgaccat gtttgtaccg caggttggtc
     5761 gtcgtctgtt tgtcgccgat caccttatag ggtgttttca cccggacgct gccgtgatcg
     5821 gtggccagga tgaggttgat cttcttgtcg gcgatcttcc gcagcgcgga atgcaatggc
     5881 gaatgttcga accagcttgc cgtgatactg cgataactga tctcgtcgct ggccagctcc
     5941 ttgagtactt ccatttcggt gcgggcatgg ctgagcatgt cgacaaaatt atagacgatg
     6001 atattgaggt cgttgttcag gagattgtgg atattggcca ccaggtcctg accggcctgg
     6061 taattcaaca ccttggtata ggagaaccgg agatgatctt tcttcaggcg cttgagctgg
     6121 gcgcggaaga attcctcctc ataaaggttc tttccgcctt cttcgtcgtc gttcttccac
     6181 tgggaaggga attgtttttc gatatccacc ggcatcaggc ccgagaagat ggcattgcgg
     6241 ctgtattgcg tggccgtcgg caggatactg taaaaactat cctcctcgag gatacgaaaa
     6301 ctctccgcga agatcggttg tatggccttc cattgatcga tgcgcaggtt atccagcagg
     6361 aggaagaagg tgggcgttcc cttttcgaca tggggaagga ccttgaactg aaaaagcgaa
     6421 tgggacatga tcggcccttc cgatgatctt ggatgcaccc agctggcata attcttcgag
     6481 aggaatttaa agaattcggt gttggcttcc tgtttctggc tttgcagcac ttcccgcatc
     6541 tcggggctat ccgatttctc catttccagc tcccagtaga tcagtttttt atagatgtcc
     6601 atccattcgt tgtagtctgg gttgctgttc agcgccatga agagattgcg aaactgctgc
     6661 tgataggcgg tggtggtctt ttccgatacc aggcgtttgt tgtcgatgat cttcttaagg
     6721 ctaagcagca cctggttggg attgacgggc ttgataagat agtccgtgat ctggctgccg
     6781 atagcatcgt ccatcagatt ctccgtctcg ttcttggtga tcagcacgac ggggatctgc
     6841 tgattggctt cttttatctt ggccagggtc tcgagaccgg tgatgccggg catggtttcg
     6901 tcgagcaata cgacatcgac gggattatcc tttacaaagt cgatggcatc gaagccattg
     6961 gtaagcgctt ttacttcgta tcctttgttt tccaggaaca ggatctggga gtgaaggctt
     7021 tcgatctcat catctaccca gagtattttg gctaatggca tatacccaca aactacaaac
     7081 tcctggccaa ttttagttac atttgacaat tgttttatcc cgttttaaca aaacagaaac
     7141 aaggatggcc caatcttcgc gtaagatcat caacgacccg gtccatggct ttattactct
     7201 cgatcatccg gtattgctga acattgtggc gcatccgtac taccagcggt tacgccggat
     7261 caaccaaatg gcttttgcgc atcttgtcta cccgggtgcg gtgcatacgc ggcttcatca
     7321 ttcgcttggc gcctatcacc tgatggggtg tgcggtgacc gagctgcaac gcaagggttt
     7381 tcccatcagc gaggaggaag cgctgggtgt aaagatcgcg atcctgttgc acgatatcgg
     7441 gcatggccct ttttcacatg ccctcgagaa tgtcctcatc cgcggcgtcc atcacgagac
     7501 gctttcgctg ctgatcatga aagtgctgaa cgaggacctg gacggacaat tggaaatggg
     7561 gatcaatatc tttacaggtg gttatccaaa acgattcctt caccagctgg tgtcgggaca
     7621 gctggatatg gatcggatgg attatctcac ccgggacagt ttttttacag gggtctccga
     7681 aggggtcatc ggctatgacc ggatcctgaa gatgttgatg gtgcacaagg gggagctgat
     7741 ggtggaagaa aaggccgtct actcgataga gaaattcctc gtcgcgcgcc ggctgatgta
     7801 ctggcaggtc tatctgcaca aaacggtggt cagcgcggag aagatgctgg tgcggatcct
     7861 ggaaagagcc cgggagctca tcgttatggg cacgcaggtg ccgtcctctt cccccgtgct
     7921 ggacttcttt ctgcaggacc gggccgaggt cctggatgca ggggcgaatt tggacaaatt
     7981 ctgcctgttg gatgactatg atgtcatgtg cacgataaag ctctggatgt tccattccga
     8041 cacggtgcta tccctgctat cccggagcct gatcgatcgc gaattactaa aagtgcggtt
     8101 ccaggcaact cccttcgatg aaaattacgt aaaggagttg agagagcggg tggcgacagg
     8161 gctgggcatc gccagggagg aggcccgcta ttttgtgttt acgggggagg ccgtcaatac
     8221 gacgtataat tcgatggatg agaagatcaa tatccttttc cgggacgcat cggtaaggga
     8281 tatttccgag gtggacaacg cactcatcca gcaaaccctg gcgagcacgg tgaaaaaatt
     8341 ttatatttgc catttgaata cc
//
LOCUS       DATGFG010000034         6542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_567586, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000034 DATGFG010000000
VERSION     DATGFG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567586"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VKQ52_00665"
     CDS             complement(<1..487)
                     /locus_tag="VKQ52_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85710.1"
                     /translation="MQLIKIARFFLLFLSCILVAAVCISQGLPIKPTRTISFSTDEGS
                     YMDVDISPDGKTLLFDLLGDLYTVPSTGGKAQQMTRGVALNLKPIWSPNGKNIAYISD
                     TSGSFHLNVIDIRRTFHRVLGKTEYPLEFQPDAVWTPDGNYLVYGEAIYGLAGGLLST
                     VA"
     gene            complement(588..1043)
                     /locus_tag="VKQ52_00670"
     CDS             complement(588..1043)
                     /locus_tag="VKQ52_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ85711.1"
                     /translation="MSDSSLKKRFLLIDDDTDDRELFSEALATVDPVIICDQATDGAE
                     AFARLRNNELSKPDLIFLDINMPVMNGWQFLTRLKSEDAYKHIPVIVYSTSSNQKDRR
                     IADDLGALCFITKPHAFRNLQHLLGVVVSHVHQKNFAAICPEVNALCVL"
     gene            complement(1036..4365)
                     /locus_tag="VKQ52_00675"
     CDS             complement(1036..4365)
                     /locus_tag="VKQ52_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ85712.1"
                     /translation="MTAIPEFLAGGGEMGQRIRDYDWAATPLGPVESWPQSLRTCVRI
                     MLTSRQPIWIGWGKELIKLYNDPYKAIVGGKHPKALGSPASVVWRDIWRHIGPMLTAV
                     MEKDEGIYVESELLIMERNGYPEETYYTFSYTPIPGDDGGTAGMICANTDDTDRIISQ
                     RQLRTLTDLGKRLADCGSYAEAIDRTILTLQENPRDFPFVLFYTLALGSATLTHSSDL
                     GIAESRVPDSIDLAGDSGEGRPGEAGGEGPLLRASGEERLVRAGSAEGLISAFRQAMA
                     TRQLQILEGLQDSMGRLPTGAWEVSPDKAIIQPMYRAGEKDPYGFLVTGVNPYRLPDE
                     KYTAFLSLVGDQVATSFADIHVLEVERRRAEALAEIDRAKTTFFSNISHEFRTPLTLL
                     ISPLEDLLAEPRPGEPGYDDGQRGKAGTDDPERGEAGTGDPERGKAEAGDSEGGKVGT
                     GDSERGKAGAGDAEQEAARRYRLGVAYRNALRMEKLVNTLLEFSRIEAGRLEGQFRRV
                     DIAALTIDLASSFRSAIEKAGMRLHVGARKIAGDVYVDVELWERIILNLVSNAFKYSQ
                     QGTISVEVRQEGGEIQVSVSDDGVGIPVEEQERIFDRFHRIENSEGRSQEGTGIGLAM
                     VRELVRLHQGTIGVISAVGVGSTFTVRIPIGVAHLPADRIISDPRGERRLRHADAFVQ
                     EAMMWVGGLDATDDGVGEGGGPQNGEARANGKGAGESPRYKVLLADDNADMREYVQRL
                     LSRQFTVITATDGEDAFAKMQEHHPDLLLSDAMMPRLDGFELLKRVRQSADLASTPVI
                     LLSARAGEEAKVEGLEAGADDYLVKPFAARELLARVDANIRIARDRSAAMKEYAEKLE
                     QAVRRRTQELRVLNNSLERSNEDLQQFAHVASHDLKEPVRKIRTFSGRLIDEYAQLLP
                     AEAQVFLKKIQQATARMYTMIDGVLNYSAINGSEQPIDTVDLNTVIDQVESDLELVIR
                     DRHATIQRGSLPCIEGAPVLLQQLFYNLLNNSLKFAKDAVNPLITIESRLTEGAGKRI
                     AELTMTDNGIGFDPGQAARIFETFVRLNTKDRYEGTGLGLSLCKKIVERHHGTITATG
                     VLGGGATFVIRLPLTQTERYV"
     gene            complement(4516..>6542)
                     /locus_tag="VKQ52_00680"
     CDS             complement(4516..>6542)
                     /locus_tag="VKQ52_00680"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HLZ85713.1"
                     /translation="LTSLTCLTAFSQSFIGYGYDNYSGVNGMILNPGTLADNKYKVNV
                     NLFSISALAGNNAYEMDRSKFFSLKYSHLTEGNGFYKANNSAYKYLYTNLDVLGPSAT
                     ITLTSKDAIGLTTRARMLGNEYNLGDPLFRLTGIANPAFYNINIVNRSLQAKATAFAE
                     AGVSYGRVLFKDDHAEIKLGITGKYIAGLGYGSLSSGPLLLNLDPTNYIANLQADVTA
                     QYSGNLDRLNGGASASDIIKHTGHGFGFDAGFVYELKGKDPNTSKLRLGVSITDIGSV
                     KYANSANSQQYTVNATGRNTQEFAEKSGESLDAYFTRLKDSGLIVSKGLKPSSTVSLP
                     TAIHVNVDYQIYKRLYINGDVLLNMLATTNPISPNYVTTFTATPRLEKKWVSIYSPVS
                     YSVNGQLNWGAGVRLGPLFVGSGSVLSNLFKNRIQAADAHIGLTIPIFQHHKNKEPKL
                     SDTAYKEKSMTNDRDGDGVVDAKDDCPDSAGPIKLIGCPDDDGDGVPNYKDKCPGVKG
                     SPNFQGCPAPDSDGDSVNDDDDKCPLVKGVASNYGCPPIKPEYVTTVKHAADRIFFVR
                     AKAVIEVVSFAELDRVVDILNEDSTLRLRIEGHTDSEGTDQRNQKLSDRRARAVYNYL
                     VFKGISPQRMDYAGFGSKKPLAPNDTPEGMAVNRRTEMILMNYPKEKSKQ"
BASE COUNT         1314 a         1907 c         1875 g         1446 t
ORIGIN      
        1 cagccaccgt tgacaataga ccaccggcaa gaccatatat agcctctcca taaacaagat
       61 aattaccatc cggtgtccac acagcgtccg gttgaaattc taaggggtat tcggttttcc
      121 ccaatactcg gtgaaatgtt ctcctaatgt cgatcacatt caaatgaaag gaccccgagg
      181 tgtcactgat gtaggcaatg tttttcccat tcggcgacca aatgggcttc agattaagag
      241 caacccctct tgtcatttgc tgagccttac ctccagtcga aggaactgta taaaggtctc
      301 caagtaaatc gaagagaagg gtcttcccat ccggtgaaat atcgacatcc atataactcc
      361 cttcatctgt agagaaagag atagtcctcg tgggcttaat aggcagaccc tgcgaaatac
      421 acacggctgc gactaatata catgaaagaa agagcaagaa gaaacgtgct atttttatca
      481 attgcatctc taaagttagt taaaagcacc tgtcgtgaca ctgcactcag ggggaatgtc
      541 tattcatacg agtaggtcac gattttaacc aatcacgaga acccttctca cagcacacaa
      601 agtgcattca cctccgggca aatagcggca aaattctttt gatgcacatg cgacaccacc
      661 acccccagca aatgctgcaa attgcggaac gcatgcggct tcgtaataaa acacaacgcc
      721 cccaggtcgt cggcgatgcg cctgtccttt tgattggaag aagtggagta tacgatgacg
      781 ggaatgtgtt tatacgcgtc ctcgctcttc agccgggtga ggaattgcca gccattcatc
      841 accggcatat tgatatcgag gaaaataaga tcgggcttgg agagctcatt gttacgaagc
      901 cgggcgaagg cttccgcgcc gtcggtggcc tggtcgcaga tgatgacggg gtccacggtg
      961 gcgagcgctt cggagaacaa ttcccgatcg tcggtgtcat cgtcgatcaa taaaaatcgt
     1021 ttcttcaaac tgctgtcaga catatctttc ggtttgagtc agtggaagcc ggatgacaaa
     1081 ggtcgcccct ccgcccagta cgccggtggc ggtgatcgtg ccatgatggc gttccacaat
     1141 ttttttgcaa agcgacaggc ccaatccggt tccctcgtag cggtccttgg tattcaggcg
     1201 cacaaaggtc tcgaagatcc gggccgcctg gccggggtca aacccgatgc cgttgtcggt
     1261 cattgtcagc tctgcaattc tcttgccagc cccttccgtc aacctcgact cgatggttat
     1321 cagggggtta acggcgtctt tagcaaattt tagggaatta ttcagcaggt tgtagaagag
     1381 ttgttgtaac agcaccggcg ccccttcgat gcagggcagg cttccgcgct ggatagttgc
     1441 atggcgatcg cggatgacga gttcgagatc gctttccacc tggtcgatga cggtgttgag
     1501 gtctaccgta tcgatgggtt gttcactacc gttgatagcc gagtaattga gcacgccgtc
     1561 gatcatcgta tacatgcggg cggtagcctg ctggatcttc ttcagaaaca cctgggcctc
     1621 cgcggggagg agttgggcat attcgtcgat caggcgaccg ctaaaggttc ttatcttccg
     1681 caccggctcc ttgaggtcat ggctcgcgac atgggcaaat tgctggagat cttcgttgct
     1741 acgttcgagt gagttgttga ggacgcgcag ctcctgtgtg cggcgccgca cggcctgttc
     1801 cagcttctcg gcatattcct tcatcgcggc gctgcggtcg cgggcgatcc ggatattggc
     1861 atcaacccgg gcgaggagct cacgggcggc aaagggcttg acaaggtaat catcggcgcc
     1921 ggcctcgagg ccttccactt tcgcctcttc ccccgcgcgc gccgagagca ggatgacggg
     1981 cgtggacgcc agatcggcgg attggcgcac ccgcttcagc aactcgaagc cgtcgagccg
     2041 cggcatcatc gcatcggaca ataacaggtc ggggtgatgc tcctgcatct tcgcaaacgc
     2101 gtcttctccg tcggtggcgg tgatgacggt gaattggcgc gagagcagcc gctgcacata
     2161 ttcgcgcatg tcggcgttgt cgtcggcgag aagaacttta taacgaggtg attcccccgc
     2221 ccccttccca ttcgcccggg cctccccatt ctgtgggccg cccccctccc cgacgccatc
     2281 atcggtcgcg tcgaggcccc ccacccacat catcgcctcc tgtacaaacg catccgcatg
     2341 ccgcaggcgc cgctctcctc gggggtcgga gatgatccgg tccgccggca ggtgagccac
     2401 cccgatagga atgcgcaccg tgaacgtcga acccacaccc accgcgctga tgacgccgat
     2461 cgtcccctgg tgcagccgga ccaattcccg caccatggcc agaccgatgc ccgtgccttc
     2521 ctggctgcgg ccttcgctgt tctcgatgcg atgaaaccgg tcgaagatcc gttcctgctc
     2581 ctctaccgga ataccaaccc cgtcgtcgct gacgctcacc tggatctcgc cgccctcctg
     2641 gcgcacctcg acgctgatgg tgccctgctg gctgtattta aaggcgttgc tgacaagatt
     2701 gaggatgatc cgctcccata attccacatc gacatataca tcaccggcga tcttccgcgc
     2761 cccgacgtgg agccgcatcc ccgccttttc gatggccgac cggaaactgc tcgcgaggtc
     2821 gatggtcagc gcggcgatat ccacccggcg gaactggcct tcgagccggc ccgcttctat
     2881 gcgcgagaat tcgaggaggg tgttgacgag cttctccatc cgcagggcat tgcgataggc
     2941 gactcccagg cgatagcggc gggcggcctc ctgttcggcg tcaccggccc cggcctttcc
     3001 tctttctgaa tctcctgtcc cgacctttcc tccttctgaa tccccagcct cggccttccc
     3061 tctttctgga tccccggtcc cggcctcccc tctttccgga tcgtcggttc cggccttccc
     3121 tcgttgcccg tcgtcgtacc ccggttcgcc gggccgcggc tcggcaagca ggtcttcgag
     3181 cgggctgatc agcagcgtca gcggggtacg aaattcatgg ctgatgttgg agaagaaggt
     3241 agtctttgcc cgatcgatct cagctagcgc ctcggcgcgc cggcgttcaa cctcgaggac
     3301 atggatatct gcgaagctgg tggccacctg gtcacccacc agcgaaagaa aggcggtgta
     3361 tttttcgtcg ggcagccggt aggggttgac gcccgtcacc aggaagccat aggggtcctt
     3421 ctccccggcc cggtacatcg gctggatgat cgccttatcc ggcgagacct cccaggcccc
     3481 ggtcggcagc cgccccatgc tgtcctgcaa cccttcgagg atctgcagtt ggcgcgtggc
     3541 catggcctgc cggaacgcgc tgatcagccc ctccgcactg ccggccctca ccagcctctc
     3601 ctcgccgctg gccctcaaca acggaccctc tccgccggcc tccccggggc ggccctcacc
     3661 gctatccccc gccaaatcga tgctatcggg aacccgggac tccgcgatcc ccaggtcgga
     3721 ggagtgcgta agtgtggcgc tgccaagggc caaggtataa aatagtacga acgggaaatc
     3781 ccgcggattc tcctgcaaag tcaggatggt ccggtcgatg gcctcggcat aactcccaca
     3841 gtcggcaagc cgcttgccca gatcggtcaa agtcctcaac tgccgctggc tgatgatgcg
     3901 atcggtgtca tcggtattgg cgcagatcat gcccgccgtg ccgccatcgt cgcccggaat
     3961 gggcgtatag ctaaaagtgt aataggtctc ttcgggatag ccgttgcgct ccatgatgag
     4021 gagctccgac tcgacataga tgccctcgtc cttttccatc accgcagtga gcatcgggcc
     4081 gatatgccgc cagatatccc gccacacgac ggaggccggg gagccgaggg cctttggatg
     4141 tttcccgccg acaatcgcct tgtaaggatc attgtagagt ttgatcagct cttttcccca
     4201 gccgatccag atgggctgac gggaggtaag catgatgcgg acgcaggtgc gaaggctctg
     4261 gggccaggac tccaccggtc ccaacggggt tgccgcccag tcgtaatcgc ggatacgctg
     4321 gcccatttcc ccaccacccg ccaagaactc cggaatcgcc gtcataaaag catatatagt
     4381 aaaattacgg gttccggcgg aatccggggg cggggggcaa aaaaagaggc ggcggacgag
     4441 gcggctccaa aaaaagaggc ctgactcttc ggggatggtc aggcctttct tatatgtagg
     4501 gctagtgggg tgcgcttatt gcttgctctt ttccttcggg tagttcatca ggatcatctc
     4561 cgtccttcgg ttcacggcca tcccctccgg cgtatcattc ggggcgagcg gcttcttact
     4621 gccaaacccg gcatagtcca tgcgctgggg gctgatcccc ttaaacacaa ggtaattata
     4681 aacagccctg gcgcggcgat cggaaagctt ctggttgcgc tggtcggtac cctcgctgtc
     4741 ggtatggcct tcgatccgga gccgcagcgt cgaatcctcg ttcaggatat ccaccacacg
     4801 gtcgagctcg gcgaaggaca ccacttcgat gacggccttg gcccgcacaa agaaaatacg
     4861 atcggcggcg tgtttcacgg tggtcacata ctcgggcttg atcggcggac agccataatt
     4921 gctggccacg cctttcacca gcggacattt atcatcgtcg tcattgacgc tgtcgccatc
     4981 gctgtcgggt gcgggacagc cctggaaatt gggcgagcct ttcacgccgg ggcatttatc
     5041 cttatagttg ggaacgccgt cgccatcatc atcgggacaa ccgatgagtt tgatgggacc
     5101 cgcactgtcg ggacagtcat cttttgcgtc aaccaccccg tcaccatcgc ggtcgttggt
     5161 catgctcttt tccttatagg ccgtatcgga gagcttcggc tccttgttct tatgatgctg
     5221 gaagatcggg atggtcagac cgatatgcgc gtccgcggcc tggatgcgat tcttgaataa
     5281 gttggaaagt acggatcccg acccgacaaa cagtggtccg aggcgaacac cggcgcccca
     5341 gttgagttgc ccgttgacgc tataggagac gggggagtag atgctcaccc atttcttctc
     5401 gaggcggggc gtggccgtaa acgtggtcac atagttgggg ctgataggat ttgtcgtagc
     5461 cagcatattc agcagcacgt cgccgttgat gtaaagccgt ttgtagatct ggtaatcgac
     5521 atttacatga atggccgtcg gcaggctcac cgtagaactc ggcttgaggc ccttggacac
     5581 gataaggccc gagtccttca ggcgagtgaa gtaagcatcc agggactcgc cgcttttctc
     5641 ggcaaactcc tgtgtgttcc ggccggtggc attgacggta tattgctggc tgttggcgga
     5701 attggcgtat ttgaccgagc cgatatccgt tatcgagaca cccagccgca gtttcgacgt
     5761 gttggggtcc ttgcccttca gttcgtatac aaaacccgcg tcgaagccaa agccatggcc
     5821 cgtgtgtttg atgatgtcgg aggcgctggc gccgccattc agccggtcga ggttaccgct
     5881 gtattgcgcg gtgacatccg cctggagatt ggcgatatag ttggtggggt cgaggttaag
     5941 gagcagaggt cccgaagaga gactgccata accaagaccg gcgatatact tacccgtgat
     6001 acccagcttg atctccgcgt ggtcatcctt gaacaggacg cggccatagg aaacgcccgc
     6061 ttccgcgaag gccgtggcct ttgcctgcag gctgcggttg acgatattga tattataaaa
     6121 ggcggggttc gcgataccgg tgaggcggaa caggggatcg ccaagattgt actcgttacc
     6181 cagcattcgg gcgcgcgtgg tgaggccgat ggcgtccttt gacgtgaggg tgatggtggc
     6241 cgagggcccg agcacatcga ggttggtata aagatatttg taagcgctgt tattggcctt
     6301 gtagaacccg ttgccttcgg tgagatggga atacttcagg ctgaagaact tgctgcggtc
     6361 catctcgtag gcattgttcc ccgcgagggc gctgatggag aacaggttga cattgacctt
     6421 gtacttattg tccgccaggg tgccggggtt gaggatcatt ccgttgacgc cggaataatt
     6481 gtcgtagcca tacccgataa aggattggga gaaagcagtc aggcaggtta aactggtcaa
     6541 ta
//
LOCUS       DATGFG010000035         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_567542, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000035 DATGFG010000000
VERSION     DATGFG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567542"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VKQ52_00685"
     CDS             <1..682
                     /locus_tag="VKQ52_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HLZ85714.1"
                     /translation="GLHFTEAIFASLPARGIRRGFVTLHVGAGTFLPVKAATLGGHIM
                     HTEFIEVGREFVLDLRATLQASRPVIAVGTTSARTLETLYWLGLKVSRSPGLPAEELV
                     VRQWDAYATNGDGAGSNGAGNDATNGHAVDAITALSILLQWMERQGIDRVITTTQLLI
                     TPGYRWKIVAGLVTNFHQPESTLLLLIASFIGEDWRKVYDYALEHGFRFLSYGDGCLL
                     LPQNPPAT"
     gene            679..1206
                     /locus_tag="VKQ52_00690"
     CDS             679..1206
                     /locus_tag="VKQ52_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HLZ85715.1"
                     /translation="MKSLAPLLLFLLLAAGVRSGIPLAGVRSTAVVRATHRDSIPRPV
                     GFVNDFEDLFTPGQKDTLEQMLHAFEQRTTIEIAVMTVDTSLTMSPNFDVFIRNTMNA
                     WGVGKKETNNGILVGISRTYRRIRIQNGYGIEKVLSNAETKKIIDEAFLPLFRKGRYY
                     EGVVNGLRALMQRLE"
     gene            complement(1258..2610)
                     /locus_tag="VKQ52_00695"
     CDS             complement(1258..2610)
                     /locus_tag="VKQ52_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ85716.1"
                     /translation="MKKIFPIIFVLISLSLIGSIYIQINWILTMLENKQEELRHKLVD
                     ATNYVSQELVAQRGGNPGKVLRLKPGLWRPSDPFTMELMRVPSVAERYTVEDITDKIR
                     KAFNLVGMKNSRFEFSVSSDPGGPGISFHPLELRSKNFLKEIEDTTNNLFAFMPLQAP
                     QNTDGDAVLPDEVLAVVVPDYKKMVLGEMRIMIGGAIFFTLVIIAAFYVTVHALVRQK
                     KLSEIKNDFINNMTHEFKTPLATISLAVDALRNEKVVQDRSKSEYFTGIIKEENKRMN
                     KQVETILQASLLDRQEQQLNLFPLHAHVIIQEAMENFHLQLEGKGGRYELQLNAKNDH
                     ILADEVHFMNIITNLIDNAVKYSKENLLIRITTHSTPRSLVIRIEDNGIGMSKETQRR
                     IFEKFYRAHTGNLHNVKGFGLGLSYVKTIVEAHQGKIKVDSVVGKGTNFTLEFPLKND
                     "
     gene            2863..>3159
                     /locus_tag="VKQ52_00700"
     CDS             2863..>3159
                     /locus_tag="VKQ52_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HLZ85717.1"
                     /translation="MESPAPGILLIADPFLKDPNFMRTVVFLCEHQDEGSFGFVLNKN
                     YEYTLDELVSGLDDLKKIPVYLGGPVQMDTIHFLHQYPDKITGGYEIIDGVFWGG"
BASE COUNT          692 a          860 c          859 g          748 t
ORIGIN      
        1 gggcctgcat tttaccgaag ccattttcgc ttcgctcccg gcccggggga tccggcgcgg
       61 ttttgtcacc ctccatgtcg gcgcaggaac ctttctgccg gtgaaggccg ccaccctcgg
      121 cggacatatc atgcataccg aattcatcga ggtcggcagg gaattcgtcc tcgatctccg
      181 ggccaccctg caggcctcca ggccggtgat cgccgtcggc accacctcgg cgcgaaccct
      241 ggaaaccctg tattggctgg ggcttaaagt ctcccgctcc cccggtctgc ccgccgaaga
      301 gctggtcgtg cggcaatggg acgcctatgc gacgaatggc gatggggccg gtagtaacgg
      361 ggctggcaac gatgcgacga atggccatgc cgtcgacgcc ataaccgcac tttcgatcct
      421 gctgcaatgg atggagcggc agggtatcga ccgggtgatc accacgacgc aattgctgat
      481 caccccgggc taccggtgga agatcgtcgc cgggctggtc acgaatttcc atcagccgga
      541 gtcgaccctc ctgctgctga tcgcctcctt tatcggggaa gactggcgaa aggtgtacga
      601 ctatgcgctg gaacacgggt tccgcttcct cagctacgga gatggatgtt tgcttttacc
      661 tcaaaatccg cccgccacat gaaaagcctg gcaccgctcc ttctttttct gctgctggcc
      721 gcgggcgtcc ggtcaggtat cccattggca ggcgtccgat caaccgctgt cgtccgggca
      781 actcaccgcg atagcatccc ccggcccgtc ggctttgtca atgactttga agacctcttc
      841 actcccggcc agaaagatac gctggagcag atgctccatg cattcgaaca acggacgacg
      901 atcgagatcg cggtgatgac ggtggacacc tccctgacga tgtcgcccaa cttcgatgtc
      961 tttatccgga atacgatgaa cgcatggggt gtggggaaaa aggaaaccaa taacggcatc
     1021 ctcgtcggca tttcaagaac gtaccggcgg atacgcatcc agaatggcta tggtatagaa
     1081 aaggtcctgt cgaacgcaga gacaaagaag atcatcgacg aggcattcct tcccctcttc
     1141 cggaaaggtc ggtattatga aggcgtggtg aatggactac gtgctttaat gcagcgactc
     1201 gagtgaaacc gcgatgaact cgcgatgatc ccacgatgat ccggggcgcc atccgttcta
     1261 atcgttcttc aacgggaact cgagggtgaa attggttccc ttgcccacca cgctatccac
     1321 ctttatcttt ccctggtgcg cctcgacgat ggtttttaca tagctgaggc ccagcccgaa
     1381 tccttttacg ttatgcaggt tacccgtatg tgcgcggtag aatttctcga agatccggcg
     1441 ttgggtctcc ttgctcatcc cgatgccatt gtcttcgatg cggatgacaa gactccgggg
     1501 agtgctatgg gtcgtgatcc ggatcaggag attctctttt gaatatttca ccgcgttgtc
     1561 gatgaggttg gtgatgatgt tcatgaaatg gacctcatcg gcgaggatat gatcattttt
     1621 tgcgttgagc tgcagttcat aacgtccgcc cttcccttcc agttggaggt gaaaattctc
     1681 catcgcctcc tggatgatga catgtgcatg caggggaaaa aggttgagct gttgttcctg
     1741 ccggtcgagg agcgaggcct ggaggatcgt ctccacctgt ttgttcatcc gcttgttctc
     1801 ttcctttatg atcccggtga agtactccga cttggagcga tcctgcacta ctttttcgtt
     1861 gcgcagggcg tccaccgcaa gggagatggt ggccagcggc gtcttgaact cgtgggtcat
     1921 gttgttgata aagtcgttct ttatctcgct gagttttttc tgccgtacca gggcgtgcac
     1981 cgtgacatag aacgccgcga tgatgaccag cgtgaagaag atggcgccgc cgatcatgat
     2041 gcgcatctcg ccaaggacca tcttcttata atcggggacg acgacggcca gcacctcatc
     2101 cggcagcacg gcatcgccat ccgtattttg aggcgcctgt aacggcatga aagcaaacag
     2161 gttatttgtc gtgtcctcta tttctttcag gaagttcttg gaacgaagtt caaggggatg
     2221 aaaactgatc ccggggccgc cggggtcgga agagaccgaa aattcaaacc ggctgttctt
     2281 catgcccacc aggttaaagg ccttgcgtat cttatcggtg atatcttcca ccgtgtaccg
     2341 ctcggcgacc gaaggcacgc gcatcagctc catggtaaag ggatcggaag gccgccaaag
     2401 acccggcttc agtctcagca ccttacccgg attgccgccg cgctgcgcca cgagttcctg
     2461 cgatacataa ttggtggcgt ccaccagctt atgccgcagt tcctcctgct tgttctccaa
     2521 catcgtcaag atccagttta tctggatata aatactgccg atcagggata gggagatcaa
     2581 aacaaaaatg atagggaaaa tttttttcaa acccatactt tttggtccat ctgcaaaatt
     2641 atcaaaagga tgaagggctg ccctgtttcg tctattcgac gagggcgatt taacgaagct
     2701 ttcacaaaga aaggggattt caggctaaaa aacgctatat ttagtatagg ggggcgccac
     2761 ccggggggcc ttcccgtaag atacgacgat gattgccatg gggaagactc attttttgcg
     2821 gaggcatttt gcaatatcgc aggattactt aatttagacg ctatggagag cccggcacca
     2881 ggcatattat tgatcgctga tccctttctg aaagatccca acttcatgcg aacggttgtg
     2941 ttcctatgcg agcaccagga cgaaggcagc ttcggattcg tcctcaataa gaactacgaa
     3001 tatacccttg acgaactggt cagcggactg gacgatctga aaaagatccc cgtctatctc
     3061 ggcggccccg tacagatgga taccatccat ttcctgcatc aatatcccga caagatcacc
     3121 ggcggatacg agatcatcga tggcgtattc tggggaggg
//
LOCUS       DATGFG010000036         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_339592, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000036 DATGFG010000000
VERSION     DATGFG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339592"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VKQ52_00705"
     CDS             complement(<1..1259)
                     /locus_tag="VKQ52_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HLZ85718.1"
                     /translation="MQLYPHNQRAYDNAVAIFRQHDKTCIIHPTGTGKAVIIAQFIQA
                     NPTARHLLLAPGEHIAAEVRKHVENTEFTFCTYNSRLSNQTFKSSRFDYIYLDEFHRV
                     GAGKWGPKVLKLLAANPDAKVLGTTATHIRFLDANRDMAFELFKGNIASYFSLNQSFL
                     QGILMAPKYINAIYSISQEYQRLEHRILQSQQEQKESLLMDLKARVIDWENSSGLDVI
                     LRKHLPPSRKKLIVFCASMKELKMAEETLTPILTSIFGSIVSLPIHSRFGREMNNRRL
                     ARFNEEDSLTKVLFTVDMVTEGLHGKDISTVILLRETNSPIIWYQQIGRCFSVGQREQ
                     PIILDLVNNFRNIQHVRFKTDYEEERAALVVHKEKGPYIRHEVQTTAVEFFDETQGIR
                     DILTSFESRIDGWMIRYRRVKAIYERQG"
     gene            complement(1479..2882)
                     /locus_tag="VKQ52_00710"
     CDS             complement(1479..2882)
                     /locus_tag="VKQ52_00710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLZ85719.1"
                     /translation="MKIAFHGAARTVTGSKHLLTLNNGKKYLLDCGMFQGMGAQTDVL
                     NRQWGFTPAEVDCLILSHAHIDHSGLIPKLVKDGFAGKIYCTPATRELTDVLLEDSAG
                     IQEDDIKYTNKKRKAEGLPYLQPLYTEEDAKRVTEHFVAVEYGSWFTIDEHVQLLYTD
                     AGHIIGSAAVSLRITEDGKETHLTFSGDVGRYKDVILRSPEVFPQADYILIESTYGNS
                     LHDPVETTPDMLLKWIQHTCLEKKGKLIIPAFSVGRTQELLFALNSLEEENRLPYLDY
                     YVDSPLSVAATEIVKNYPQYFNKQIQKVLETDKDPFAFRGLKYIKTVEESKLLNFRDT
                     PCVIISASGMADAGRVKHHINNNIGNSRNTILLVGYCEPHSLGGRLMAGAKEVHIFGV
                     LHEVHAEVGSIRSMSAHGDYEDLSQFLACQDPKKVKMLFIVHGEYEVQQAFQARLIKK
                     GFANVQIPELHSEHGLG"
     gene            3237..>4292
                     /locus_tag="VKQ52_00715"
     CDS             3237..>4292
                     /locus_tag="VKQ52_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85720.1"
                     /translation="MPFLFFCFFSGRSYAAPPGPDGLGDRHGSPGHALHEEPVKPTPR
                     RVGDRLYFTPACPIIAENFNETADVNGATSDPSSVGWYLDASKVPSAVYFAVKSHRIK
                     AQTLGGEGVWYSQVFNISGYTGIQVDAKISAEGSFTSSEYVKVSYILDGGPETLIGEY
                     TGNFGTPLVTSAQMTGSRVQIVIRLYNVSAGNNDYYIEQYDVFKEVGPCTVSGITVSA
                     AASNSGVLTCTNPSTTLTASTTASGTTTYSWTGPNSFTATGASVTATAAGTYTVTGTN
                     SAGTGTASVTVTSNTAAPAGVTATASGSLTCATNSVTLTGSSTTAGTTFAWTGPSGFT
                     ATGATATATAAGTYTLTA"
BASE COUNT          923 a         1027 c         1257 g         1085 t
ORIGIN      
        1 ccctgccgct cataaattgc ctttactctc ctatatctta tcatccatcc gtctatccgg
       61 ctttcgaagg aggttagtat atcccttatt ccctgtgttt catcgaaaaa ttcaactgca
      121 gtagtctgta cctcatggcg aatatatggt cctttctctt tatgaaccac cagggccgct
      181 ctctcttctt cgtaatcggt cttaaaccga acatgttgaa tatttctgaa attatttacc
      241 agatccagga ttatgggttg ttctctttgc cctacggaaa aacatcttcc gatctgctga
      301 taccagatga tcggcgagtt agtctcccgt agtaagatca ccgtggaaat gtcctttccg
      361 tgaaggcctt cggtaaccat atccaccgtg aaaagaactt ttgtcaggct atcttcttca
      421 ttgaatctcg cgagacgccg attattcatt tccctaccga atctactatg aataggcaag
      481 gaaacgatgc ttccgaaaat gcttgtgaga attggagtga gcgtttcctc ggccattttc
      541 aattccttca tggacgcaca aaaaacgatg agcttctttc tggaaggcgg cagatgtttt
      601 ctcaggataa catcgagacc gctgctgttc tcccaatcga tgacgcgggc cttcagatcc
      661 atgagcaggg actccttttg ttcttgttga gactggagga tccgatgttc caatcgttgg
      721 tactcctgtg agatggagta aatggcgttg atatattttg gcgccatcaa aatgccttgc
      781 aggaatgact ggttgaggga aaaataagaa gcgatattgc ccttgaacag ttcaaaagcc
      841 atgtcgcgat tggcatcgag gaatcggata tgagtggcgg tggtgccgag gactttggca
      901 tccgggttgg ctgcaagcaa tttgaggacc ttggggcccc attttcccgc tcctacgcgg
      961 tggaattcgt cgaggtagat atagtcgaag cgggaggatt tgaatgtttg gttggataag
     1021 cggctattat aggtacagaa ggtaaattcg gtgttttcga cgtgttttcg aacctcggcc
     1081 gcgatgtgtt caccgggggc gaggagcagg tggcgggcgg tggggttggc ctggatgaat
     1141 tgggcgatga tgacggcttt gccggtaccg gtgggatgga tgatgcaggt tttatcgtgt
     1201 tgccggaaga tggcgacggc gttgtcataa gcccgctggt tgtggggata aagttgcata
     1261 gggtaaagtt taatgttctg ctaaaataag aaaatcataa aatcccgggt tgcgatttat
     1321 caaaaaataa tcaggggcgg ggtaccctgc agcgatggcg caggaaggtg acccggaaga
     1381 gcgagggagg gcgcggctgc ggggggcgga cagttagccg aggcgtgcgt tggcgggtgc
     1441 gcggtgcggg gccccgtcag tttgcggggg gcggacagtt agccgaggcc gtgttcggaa
     1501 tgcaattcgg gtatctgcac gttagcgaat ccctttttga tcaggcgggc ctggaaggct
     1561 tgctgaacct cgtattcgcc atggacgata aaaagcattt tcaccttttt cgggtcctgg
     1621 caggcgagga actggctgag atcttcatag tctccgtggg cgctcatgct gcggatgctg
     1681 cccacctcgg cgtggacctc gtgcaagaca ccgaagatgt gcacttcctt cgcgccggcc
     1741 atgaggcggc cgccgaggga atggggttcg cagtacccga cgaggaggat ggtgttacgg
     1801 ctgttaccga tattgttgtt gatatggtgt ttgaccctac ccgcatcggc catgccgctg
     1861 gcggagatga tgacgcaggg cgtatcgcgg aagttgagca atttggactc ttccacggtc
     1921 ttgatatatt tcagcccacg gaaagcgaag gggtctttgt ccgtctcgag tactttttgg
     1981 atctgcttat tgaaatattg cgggtagttc ttgacgatct cggtagctgc gacgcttagt
     2041 gggctgtcga cataataatc gagatagggc aggcggtttt cttcttcgag ggaattgagg
     2101 gcaaagagaa gttcctgggt gcggccgacg ctgaaggcgg gtatgatgag tttgcctttc
     2161 ttttcgagac aggtatgctg gatccattta aggagcatat cgggtgtcgt ctccacggga
     2221 tcgtgaaggc tgttgccata ggtggattcg atcaggatat aatcggcctg gggaaatacc
     2281 tcgggcgaac ggaggatgac atccttataa cgaccgacgt cgccgctgaa ggtgaggtgg
     2341 gtctctttac cgtcctccgt aatccttaga gagacggcag cgctgccgat gatatggccc
     2401 gcatcggtgt agagcaactg gacatgttcg tcgatggtga accaggagcc atattccacc
     2461 gctacgaagt gttccgtcac cctcttagca tcttcttccg tgtagagggg ttgcagatag
     2521 ggcagcccct cggcctttct tttcttattg gtatacttaa tatcgtcctc ctggatgcct
     2581 gcactgtcct cgaggaggac gtcggtgagt tccctcgttg cgggagtgca gtatatcttg
     2641 ccggcgaatc catccttcac cagtttgggg atgagaccgc tgtgatcgat atgggcgtgg
     2701 gagaggatga ggcaatcgac ctcggcaggc gtaaagcccc attggcggtt gagcacatcg
     2761 gtctgggcgc ccataccctg gaacatacca cagtcgagca ggtatttttt accgttgttg
     2821 agcgtgagta gatgttttga gccggtgacc gtgcgggcgg cgccgtggaa agcgatcttc
     2881 atgtcgttgg attatcgggg taaatgtagg gaaaaaccgt ttataaaata atcatttctt
     2941 tactactcag attcctatta agcggatgcg agaagattca tcactttagg gttatatatt
     3001 tcctattctg tcataccgcg ggcattcctt ttccggcgcc acattgagac gttgtcggga
     3061 ttttcgcagt cacaaaaaaa tgtactttgc cttctcgttt atagtatata gttaccctta
     3121 tccttcaggc gccgcctgcc ggaaagcgag cttccgctac cggcgacata accccgtgaa
     3181 ctaaaccaat acatatgaac atttgtctac ctggaagaag gtgtaatatc cttattatgc
     3241 cgttcctttt cttttgcttt ttttcggggc ggtcgtacgc cgcgccgccg ggacccgatg
     3301 gtttgggcga caggcacggt tcgccgggac atgccctcca tgaagagccg gtgaagccca
     3361 cacccagaag ggttggcgac cggctgtatt ttacacctgc ctgtccaatc atcgcggaga
     3421 acttcaatga aaccgcagac gtcaatgggg caacgtcaga ccccagttcg gtgggatggt
     3481 atctcgatgc atcaaaggtg ccaagtgcag tgtattttgc cgtgaaatcc catcgtatca
     3541 aggcgcagac attaggcgga gagggcgtct ggtattccca ggtgttcaac atatccggct
     3601 atacgggcat tcaggtggat gcaaagatct ccgccgaagg atccttcacg agctcggaat
     3661 atgtgaaggt gtcgtatatc ctggatggcg gcccggagac gctgatcggg gagtacacag
     3721 ggaactttgg gactcccctt gtcacctcgg cgcagatgac gggaagcagg gtccagatcg
     3781 tcattcggtt gtacaatgtg tcggcgggga ataacgatta ttatatcgag cagtacgatg
     3841 tcttcaagga agtcgggcct tgtacggtgt cggggatcac ggtgtcggca gccgcgagta
     3901 attcgggggt gttgacttgt accaatcctt cgacgacgtt gaccgcctcc acgacggctt
     3961 ccgggaccac cacctatagt tggacaggcc ccaatagttt taccgccacc ggggcatctg
     4021 taacggcaac tgcagccgga acctatacgg tgacggggac caattcggcg ggcacaggga
     4081 cggcctctgt gacggtgacc tccaatacag cggcgccggc gggtgtgaca gcgacggctt
     4141 ccggctcgct gacttgtgcg accaacagtg taacgctgac gggcagctcg accactgcgg
     4201 ggacgacctt cgcctggacg ggtcccagcg gctttaccgc caccggcgct accgctacgg
     4261 ctaccgcggc gggcacctac acgctgacgg cg
//
LOCUS       DATGFG010000037         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_109623, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000037 DATGFG010000000
VERSION     DATGFG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109623"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..921)
                     /locus_tag="VKQ52_00720"
     CDS             complement(184..921)
                     /locus_tag="VKQ52_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ85721.1"
                     /translation="MLLKAVIVDDEPVARKVIKEYIEDIEWIELAGMAENPLKADALL
                     SGGQVQLLFLDINMPKLSGIEFLRASAGHPNQPMVIITTAYAEYALDGFELDVVDYLV
                     KPFSFERFLRACNKARSKYGERFPAERVPERDERADHFFVKCGSSLEKVLYDELEYVE
                     ALTNYIVLHTTGRQLIVYLTLKGVLGHLPAERFVKVHKSFIVNKDRIKSIRGNMLQLG
                     GAEIPVSQHYYDAAMQEILKDRVIKRE"
     gene            complement(890..1942)
                     /locus_tag="VKQ52_00725"
     CDS             complement(890..1942)
                     /locus_tag="VKQ52_00725"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ85722.1"
                     /translation="MKRHRIWISIGLWVGLYLCWITLFRDRVFTVTRTLTVQFCYLLF
                     VAVNYYAQLHYAIPRLLNRKKYLAFALFLPGAITLSALLRTPLAIWLQVSFFRPGSPA
                     PDTATVFRDSFLNIFIWVMCLVSIRLVLEKVWFRQYMDTMEKERIRNELDFLKAQFNP
                     HFLFNSINSIYGHIDRKNGKARNMLLSFSEMLRYQLYECDVDNIVIEKEIQYIRNYIA
                     LQQERKEENLHVQVDIADDVRGFTIAPLLLITFVENAFKYVSSYEDGENRVIISLRRE
                     GEQLLFRAYNTRDEGMVRLEMEKGGIGIANARRRLALQYPGKHQLQITDGGESFEVRL
                     HLNIKPHVVESRYSRR"
     gene            2041..2514
                     /locus_tag="VKQ52_00730"
     CDS             2041..2514
                     /locus_tag="VKQ52_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85723.1"
                     /translation="MKAATIHEIKQELQSLSPTRLSELCLRLAKFKKDNKELLTYLLF
                     EAADETTYIESIKKEIDEEFGGLPKPNLYLTKKSLRKVLRVTAKQIRYTGSPQAEVEL
                     LTYFLRKLKRSGIPYADSPVLVNLYRQQLKKVRAVIAGMHEDLQYDYLKELQGLD"
     gene            2533..>3055
                     /locus_tag="VKQ52_00735"
     CDS             2533..>3055
                     /locus_tag="VKQ52_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HLZ85724.1"
                     /translation="MNKRPAFFAALLFTVVTAGAQSPADRQKVEALLRQMTTDEKIGQ
                     MTQVTLGVVSAPEDGVLDPAALAKAVNEYKVGSILNVTNHALTVDQWRRVQTQIQDEA
                     KKTRLKIPVIYGLDGIHGQTYTLDATLFPQNIGMAATRNPELAAAVTKVTAKELRASG
                     VRWNFAPVLDCGRQ"
BASE COUNT          735 a          861 c          819 g          640 t
ORIGIN      
        1 ccgctttttg caatgccccg cggatggcga cggcgccgag ccggggtgct gaaaggtctt
       61 ttaacccacc gccaaaactg cctatcggcg tccttacggc cgcgacaatg acgacttctt
      121 tgttcatgac tttttttcgg attttgggca tgaagataag aataaaaact aacaattgtt
      181 agtctactcc cgtttgatga cacggtcctt caggatctcc tgcatcgccg catcataata
      241 gtgctggctg acggggattt ccgcccctcc cagttgcagc atattgcccc ggatactttt
      301 tatcctgtcc ttgttcacga taaagctctt gtggaccttg acgaaccgct ccgcgggcag
      361 gtgcccaagc acccccttca gcgtaagata aacgatcagc tgacggcccg tggtatgcag
      421 aacgatatag ttggtcagcg cttcgacata ctccagctcg tcgtacagca ctttctccag
      481 gctgcttccg catttgacaa agaaatgatc cgcccgctca tcccgctccg gcacccgttc
      541 ggcgggaaac cgctccccat acttgcttct tgccttattg caggcccgga gaaaacgttc
      601 gaaagagaag ggttttacca ggtagtcgac gacatccagt tcgaagccgt caagggcgta
      661 ttcggcatag gccgtggtga tgatgaccat gggttggtta ggatggcccg cggaggcacg
      721 aaggaactcg atgccgctga gttttggcat attgatatcg aggaacaaca gctgcacctg
      781 tcccccgctg agcagggcat ccgccttcag cgggttctcc gccataccgg ccagctcgat
      841 ccattcgata tcttcgatgt actcctttat taccttcctg gccaccggtt catcgtcgac
      901 tataacggct ttcaacaaca tgcggtttta tgtttaaatg taaacgcact tcaaaggact
      961 ccccgccatc ggtgatctgc aactggtgtt tacccggata ttgcagggca aggcggcgcc
     1021 gggcattggc gataccgatg cccccctttt ccatctcgag ccgcaccatc ccctcatccc
     1081 gggtgttgta ggcccggaac agcaattgtt ccccttccct tcgcagggaa atgataaccc
     1141 tgttctctcc gtcctcataa ctgctcacat acttgaaggc attctcgaca aaggtgatca
     1201 gcaacagggg ggcgatggta aacccccgga catcatcggc gatatccacc tggacatgaa
     1261 ggttctcctc cttccgttcc tgttgcaggg cgatataatt ccggatatac tggatctcct
     1321 tctcgatcac gatattgtcc acgtcgcatt cgtacagctg gtagcgcagc atctccgaaa
     1381 agctaagcag catattcctt gccttgccat tcttcctgtc gatatggcca taaatggaat
     1441 tgatggaatt gaaaaggaaa tgcggattga actgcgcctt caggaaatcg agctcgttgc
     1501 ggatgcgttc cttctccatg gtgtccatat actgcctgaa ccagaccttc tccagcacaa
     1561 ggcgtatcga aacaagacac atcacccaga taaagatatt cagaaagctg tcgcgaaaca
     1621 ccgtcgcggt atcgggagcg ggagagcccg gacggaaaaa ggacacctgc agccagatgg
     1681 ccaggggagt ccggagcagg gcgctcaggg tgatggcgcc cggcagaaaa agggcaaagg
     1741 cgaggtattt cttgcggttg agcagccggg gaatggcata atgcaactgg gcgtagtagt
     1801 tgacggcgac gaacaggaga tagcagaatt gcaccgtcag ggtccgggtg accgtaaaga
     1861 cccggtcgcg gaacagcgtg atccagcaga ggtacaggcc gacccagaga ccgatggata
     1921 tccagatacg atgtcgcttc attcgcccga tttgatgagg tactcataaa ggtaggagga
     1981 tcggcgggga aagaaagcgt ttttacacca aaggagcgaa aaccttgata acttgcggac
     2041 atgaaagcag cgaccattca cgagatcaaa caggagctac aatccctgtc ccctacccgg
     2101 ttgtcggaac tctgcctgcg gctggccaaa ttcaagaaag acaacaagga gctgctcacc
     2161 tacctgttgt tcgaggcggc ggatgagacg acttatatcg aatccataaa aaaggagatc
     2221 gatgaagagt ttggaggcct gccaaaaccc aatctttacc ttaccaagaa aagcctgcgg
     2281 aaggtgttgc gggtgacggc caaacagatc cgctacaccg gctcgccgca ggcggaggtg
     2341 gagttgctca cctatttcct ccggaaactg aaaagaagcg gcatccccta tgcggacagc
     2401 cccgtgctgg tcaatctcta ccggcagcag ttgaaaaaag tgagggcggt gatcgcggga
     2461 atgcacgaag accttcaata tgactatttg aaagagttgc aggggctgga ttgatcccct
     2521 acctttaacc tcatgaacaa acgccccgcc ttcttcgccg cccttctttt taccgttgta
     2581 acggccggcg cccagagccc ggccgatcgc caaaaagtgg aagccctcct caggcaaatg
     2641 accaccgatg aaaagatcgg ccagatgacc caggtgaccc tgggggtcgt ctccgccccg
     2701 gaggacgggg tcctggaccc cgcagcgctg gcaaaggcgg tgaacgaata caaggtgggc
     2761 tccatcctca acgttaccaa ccatgccctc acggtggacc agtggcggcg ggtgcagaca
     2821 cagatccagg acgaggcgaa aaagacccgg ctgaagatcc cggtgattta cggactcgac
     2881 ggcatccacg gacagaccta taccctggat gcgaccctgt tcccccagaa tatcggtatg
     2941 gccgcgacac gcaaccccga actggccgcc gcggtaacaa aggtcaccgc aaaggaattg
     3001 agggcctccg gggtacgctg gaatttcgca ccggtgctgg attgcggccg ccagc
//
LOCUS       DATGFG010000038         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_793064, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000038 DATGFG010000000
VERSION     DATGFG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793064"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VKQ52_00740"
     CDS             complement(<1..269)
                     /locus_tag="VKQ52_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85725.1"
                     /translation="MITQAICWTLLHSLWQGALAALVAGIIIHCTRRSRAVIRYNFLV
                     ADLLFFLMVAGCTFFYSLTPAHGGVAPVRSGAAAKPENEVFIRLMP"
     gene            complement(266..673)
                     /locus_tag="VKQ52_00745"
     CDS             complement(266..673)
                     /locus_tag="VKQ52_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HLZ85726.1"
                     /translation="MKSEKSRSTEKTEPTRSELEILQVLWQYGPNTVRFVNERLNEDK
                     REVQYASTLKLMQIMVDKRLLHRDESQVKHIYSPASEEYKTKSLLLDRFVDSLFNGSV
                     SSLMLHLLDNKKASQTELDAIRELLDKAKKDKK"
     gene            complement(715..>2249)
                     /locus_tag="VKQ52_00750"
     CDS             complement(715..>2249)
                     /locus_tag="VKQ52_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HLZ85727.1"
                     /translation="HWEDRGKINTLYESLPVIAFDGRQTIWASHPYRGVFRNPLSGPY
                     RQYGVAGGLPSLRNNYIVEWKNKILAATERGVYEYDPEADRFVPSAHFRPIFGDTSVE
                     YLHPDRDGNIWFVSDQRVGVIDYGKPSGAARYSVVYFPELKGQTVKGAPMIYPYDKEN
                     VFIGSNNGVFHLNYSRWLESAEKIRVLLGAVKAIGPRDSLIAGGYGGDTQAVVRLPNH
                     WNSFHFEYASPLFTQPNAVEYSYRLAGFDHDWSEWSARSEKDYTNLPQGQYRFQVRAR
                     DNLGNVSEPAGFSFIVNPAWYQSGWAYLAYLLLAGGLFYLVRRQQQRRLLLHQKKYQE
                     EQERQNYLHSLELDRKEKGLIALQNEKLAGELQFRNKELATVTMHLVERGGILSSIKE
                     ELTAVIRRLNVPNLSHEFKPVFRMISDTEKNDDDWNRFSLYFDEVHNNFLGTLKTKYP
                     QLSPTDLKLCAYLRLNLSSKEIAQLLNISLKGVEVSRYRLRKKLGLATEVNLTDFLGK
                     IS"
BASE COUNT          487 a          690 c          559 g          513 t
ORIGIN      
        1 ggcatcagcc ggataaaaac ctcgttctcc ggtttcgccg cagcgccact ccgcaccggc
       61 gccactccgc cgtgcgccgg agtcagcgag taaaagaagg tgcacccggc caccatcaga
      121 aagaacaaca ggtcggctac aaggaaatta taccggatga cggcacggct cctgcgcgta
      181 caatggatga taatccccgc cacaagggcg gcaagtgccc cctgccacaa agaatgcagc
      241 agtgtccagc agatcgcttg cgtgatcatt ttttatcttt tttggctttg tccaatagtt
      301 cccggatggc atccagctcc gtctgcgacg ctttcttgtt atccagcaaa tgcagcatca
      361 gactgctcac cgatccgtta aatagcgaat cgacaaaccg gtccaataac aggctcttgg
      421 tcttatattc ttcggaggcg ggactgtaaa tatgcttgac ctggctctcg tcccggtgta
      481 acaacctctt gtcgaccatg atctgcatca gcttcagcgt agaggcatac tgcacctccc
      541 gtttgtcttc gttcagccgt tcattgacaa agcggaccgt gttgggacca tactgccaca
      601 aaacctgcag gatctcgagc tctgatcgcg tgggctctgt cttttcggtg gatctcgact
      661 tttcggattt catggtccta aggtaggaaa cttttcctac tatccaaata aaatctagga
      721 aatttttcct aggaaatcgg taaggttcac ttccgtcgcc agtcccagct tcttccgcag
      781 ccggtaccgg ctcacctcca cccccttcag cgagatattc agcagctgcg cgatctcctt
      841 ggaagacagg ttcaagcgca gataagcaca cagtttcaga tccgtcgggc tcagctgtgg
      901 gtatttcgtc ttcagcgtcc ccaggaaatt gttatgcacc tcgtcgaagt agagcgaaaa
      961 ccggttccag tcatcatcat tcttctccgt atcgctgatc atccggaaca ccggcttgaa
     1021 ctcgtgcgac aggttcggca cattcaatcg cctgatcacg gccgtcaatt cttcctttat
     1081 actcgacagg atcccgccgc gttcgacaag atgcatggtc acggtcgcca gctccttgtt
     1141 cctgaactgg agctcgccgg ccaacttctc gttctgcaat gcgataagcc ccttctcctt
     1201 gcggtcgagc tccaggctat gcagatagtt ctggcgttcc tgttcttcct ggtatttctt
     1261 ctgatgcagg agcagccggc gctgttgctg ccgccgcaca agataaaaaa gcccccctgc
     1321 cagcagcaga tatgccagat aggcccagcc gctctggtac caggcggggt tcacgataaa
     1381 gctaaaaccg gccggttcgg atacgttccc gagattatcc cgcgcccgca cctggaagcg
     1441 gtattgcccc tgcggaaggt tggtgtagtc tttttccgat cgcgccgacc attccgacca
     1501 gtcatggtcg aaacccgcca gccggtagct gtactcgacg gcgttgggct gcgtaaaaag
     1561 cggtgaagca tattcaaaat gaaaggagtt ccaatgattc ggcagtcgca cgacagcctg
     1621 ggtatccccg ccgtacccgc cggcgataag actatcccga gggccgatag ccttcaccgc
     1681 cccgagcaac acccggatct tctcggccga ttccagccat cggctatagt tcagatggaa
     1741 caccccgtta ttcgatccga taaagacatt ctccttgtca tacgggtaga tcatcggtgc
     1801 gcccttcacc gtctgcccct tcagttccgg aaaatacacc actgaatacc gtgccgctcc
     1861 cgagggcttg ccatagtcga tgacccccac ccgctggtcg ctgacaaacc agatgttccc
     1921 atcccggtcc gggtggagat attccacgct ggtgtcgcca aagatgggcc tgaaatgggc
     1981 cgagggcaca aaccggtcgg cttccggatc atattcatat accccccgct cggtcgccgc
     2041 aaggatcttg ttcttccact ccactatata attattgcgc agcgagggca gccccccggc
     2101 aaccccatac tgccggtagg gccccgataa ggggtttcgg aagacgcccc ggtaaggatg
     2161 cgaggcccag atggtctgcc ggccgtcaaa agcgatcacc ggcagcgact catacagggt
     2221 attgatcttt ccccggtctt cccaatgac
//
LOCUS       DATGFG010000039         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1022492, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000039 DATGFG010000000
VERSION     DATGFG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022492"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VKQ52_00755"
     CDS             <1..1276
                     /locus_tag="VKQ52_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85728.1"
                     /translation="HQFLETAGVITGYDAQGLPIYNDFFLCFDPHERLYLNGNWQTDL
                     IPRLGLPPADQAEITRFLQQMEQFRLAKGADGRDAFTIPVDNSSKDATYTALDSLTME
                     QWLEQQQLRSAYLRWYVNYCMRDDFGTTAAQISAWTGIHYYASRKGKGANARHYDVLT
                     WPQGNGWLVEQLCRPIGDKLKPNALAIRIVPSAGGTAATHHPAAAHSPAEVHYYDTVA
                     HQVKALHAKHVIVAVPQFVANRLLPVEGERARIVKEEMHYTPWMVANLTVGNLVERTG
                     APLSWDNVLYGGESLGYVEATHQQIQQLKEKKVLTYYWPLTHTDPAAARQWAFQRKHA
                     DWVGDIVGELRKVHPDIEEQTENIDIMLWGHAMTQPRPGWIHGGHRQLLQESYSPNIH
                     FAHTDLAGISIFEEGFYQGIKAANKILGGPGA"
     gene            1273..2556
                     /locus_tag="VKQ52_00760"
     CDS             1273..2556
                     /locus_tag="VKQ52_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85729.1"
                     /translation="MITTPPTPGPSGAPTPPADTAPTTAPTSTSGAPPIPPIGPLPAD
                     THRQPWLGRPAIETIFILLPPFLSLAFIAAFPALFHDNGNIPDAWWVILILLIDVAHV
                     YSTLYRTYLDPVSFRRYRVPLLLIPLLGFIGGTLLYWWDGLLFWRVLAYLAVFHFIRQ
                     QYGFMRIYSRQELAPSTAPSAAAAHPSPVPRPSPSANRTPPRPSPSWARRIDTLTIYY
                     ATLYPLLYWHLGEPRHFNWFVDDDFLLFRSTAILQIATLFYWLVLAAWLIREAIQLIQ
                     TRHLNIPRVAILSGTILSWYFGIIYFNGDMAFTLLNVVSHGIPYMALIWIYGQRQVRP
                     TPLLQKVFSPYGILLFLGIIFLLAYLEEGLWDMTVWKEHVKLFSVFHLFGGRLHEDAL
                     IFVVPLLALPQLTHYIIDGFIWRSGGSARRRQQSN"
     gene            complement(2507..3421)
                     /locus_tag="VKQ52_00765"
     CDS             complement(2507..3421)
                     /locus_tag="VKQ52_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85730.1"
                     /translation="MPRILVAIVFAFSALSLTLPVCAQSTDGRGTPPPDGTPVQPTTS
                     LTGSGQQPVGQSAMGGTQPVVASRRPPEAPQSAEQIVAHHIEALGGKEKLQGLNSLYL
                     EGKAILPSGLEINTRSWRVYDRLFRQELSFGESHIIIVATPGKGWAASPRTGGEFKAL
                     SPEDLKALRVEIDPAGPLADYSQKGFKLEKAGVDTINGEPCYKVKVSCPSNHSITYYI
                     DENNWYVLRETRRGGGLVGCGSEITGWNGASDGSVTIDYADYKKGPGGYVLPYSITVS
                     GLGVPITIQKVEINSTVDVDGLSRPNAR"
BASE COUNT          717 a         1220 c          931 g          726 t
ORIGIN      
        1 ccatcaattc ctcgagacgg ccggcgtcat tacgggttac gatgcgcagg ggctgcccat
       61 ctataacgat ttttttcttt gcttcgatcc gcacgaacgg ctttatctga atggaaactg
      121 gcaaaccgat ctgatccccc gcctcggcct cccccccgca gaccaggccg agatcacgcg
      181 cttcctccag cagatggagc agttccggct cgcgaaaggc gccgacggcc gcgacgcgtt
      241 taccatcccc gtcgacaatt cgagcaagga tgcgacctac acggcactcg atagcctcac
      301 catggagcaa tggctcgagc agcagcagct tcggagcgcc tacctccgct ggtatgtcaa
      361 ctactgcatg cgcgacgact tcggcactac ggccgcgcag atcagtgcat ggaccggcat
      421 tcattattat gcctcgcgaa aggggaaggg cgccaatgcc cggcattacg acgtcctcac
      481 ctggccgcag gggaatggct ggctggtgga gcagctgtgc cggcccatcg gcgacaagct
      541 aaaacccaac gccctcgcca tccgcatcgt tccgtcagcg ggcggaaccg cggcgacgca
      601 tcatcccgca gcggcacatt cccccgcaga ggtccactat tacgacacgg tcgcacacca
      661 ggtaaaggcc ctccacgcga agcacgtcat cgtcgccgtt ccgcaattcg tcgccaaccg
      721 cctcctgccc gtcgaggggg aacgcgcccg cattgtgaag gaggagatgc actatacacc
      781 ctggatggtg gccaacctga cggtgggcaa tctcgtcgag cggacgggtg ctccgctgag
      841 ttgggacaac gtgttgtatg gcggcgagtc gctgggttat gtcgaggcga cgcaccagca
      901 gatccagcag ctaaaggaaa agaaggtgtt gacatattac tggccgctga cccataccga
      961 cccggcggcc gcgcggcaat gggccttcca gcggaagcat gccgactggg tgggggacat
     1021 cgttggcgaa ctgcggaagg tgcaccccga catcgaggag cagaccgaga acatcgacat
     1081 catgctctgg gggcatgcca tgacccagcc ccggccgggt tggatccacg gtggtcaccg
     1141 ccaactgctg caagaatcgt acagcccgaa tatccatttt gcgcacaccg acctggcggg
     1201 gatcagcatc ttcgaagagg gcttctatca gggcatcaag gccgccaaca aaattttggg
     1261 agggcccggc gcatgatcac cacaccacca acgcccggcc ccagcggcgc accgaccccg
     1321 ccggccgata ctgcaccaac caccgcgcca accagcacca gcggcgcacc ccccatccca
     1381 cccatcgggc ccctacccgc cgacacccac cgccaaccct ggctcgggcg ccccgctatc
     1441 gaaacgattt tcatcctcct gccccccttt ctgagccttg cctttatcgc agccttcccg
     1501 gcgctattcc acgacaacgg caatatcccc gacgcctggt gggtcatcct catcctgctg
     1561 atcgacgtcg cccacgtcta cagcacgctc taccgcacct acctcgaccc tgtgtccttc
     1621 cgccggtatc gtgtacccct gctcctcatc cccctgctcg gattcatcgg cggcacgctc
     1681 ctctattggt gggacggcct cttattctgg cgcgtcctcg cctatctcgc ggtatttcat
     1741 tttattcggc agcaatatgg atttatgcgc atctatagcc ggcaggagct tgcgccatcc
     1801 acggccccgt ctgcagcagc ggcacacccc tcacccgtgc cccgtcccag cccctcggcc
     1861 aatcgcaccc cgccccgccc ctcaccatcc tgggcccgcc gcatcgacac cctcaccatc
     1921 tactacgcca ccctctaccc cctgctctac tggcatctcg gcgagccacg gcatttcaac
     1981 tggtttgtcg acgacgactt cctgctgttc cgctcaacgg caatactaca gatcgccacc
     2041 ctcttctact ggctcgtcct cgccgcctgg ctcatccgcg aagccatcca actcatccag
     2101 acccgccatc tcaatatccc gcgcgtcgcg atcctgagcg gcaccatcct ctcctggtat
     2161 ttcggcatca tctatttcaa cggggacatg gcatttaccc tgctgaacgt cgtcagccac
     2221 ggcattccgt atatggcgct gatctggatc tacggccagc ggcaggtccg gccaacccct
     2281 ctattacaaa aagtcttcag cccctatggc atcctcctct tcctcggcat catcttcctc
     2341 ctcgcctatc tcgaagaagg cctctgggac atgaccgtct ggaaagagca cgtcaaactc
     2401 ttctccgtct tccacctctt cggcggccgg ctacacgagg acgcgctcat cttcgtggtc
     2461 ccgctcctcg cgctaccaca actgacgcac tatatcattg atggatttat ctggcgttcg
     2521 ggcggctcag cccgtcgacg tcaacagtcg aattgatctc taccttctgt atcgttatcg
     2581 ggacgcccag cccactaacg gtaatggaat agggcagtac atagccgccc ggaccttttt
     2641 tgtagtcggc atagtcgatg gtcacgctcc cgtcgctggc gccgttccag ccagtgatct
     2701 cgctgccgca gcccaccagg ccgccgcccc ggcgcgtctc gcgcaggaca taccagttgt
     2761 tctcatcgat gtaataggtg atggaatggt tggagggaca ggaaaccttc accttataac
     2821 agggctcgcc gttgatcgtg tcgaccccgg ccttttcgag cttgaaacct ttttggctgt
     2881 agtccgccag gggtccggca ggatctattt ctacccgcag tgccttcaag tcttccggag
     2941 acagggcctt gaactcaccg cccgtacggg gacttgccgc ccaccccttg ccgggcgtag
     3001 cgacgatgat gatatggctt tcgccaaaac taagctcctg gcgaaagagc cggtcataga
     3061 ccctccagct ccgggtattg atttccagtc cactggggag aatagccttt ccttcgagat
     3121 aaagggagtt gagcccctgc aatttctcct tcccgccaag ggcttcgata tgatgggcaa
     3181 cgatttgttc agcggactgc ggggcctccg gcgggcggcg gctggcgaca accggctggg
     3241 taccccccat ggcagattgg ccgacaggct gttggccact cccggtcaaa gaagtggttg
     3301 gctgaacagg ggttccgtcc ggcggaggag ttccccttcc atccgtggat tgcgcgcaca
     3361 cgggaagggt gagggatagg gcggaaaagg caaaaacaat cgcgacaagg atacgaggca
     3421 tatatgtagg gtttgacaat tctccaatat cctaaattta acgttatttc gcgttaaatt
     3481 attagttaat ctgatgcaaa ttcgtggcca actcgtcgat attctcgaac aaaagatcaa
     3541 accagtcgaa atcacgatca tagacggtat tatcgactcc atcacccccg tccc
//
LOCUS       DATGFG010000040         8128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_451136, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000040 DATGFG010000000
VERSION     DATGFG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451136"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3440
                     /locus_tag="VKQ52_00770"
     CDS             <1..3440
                     /locus_tag="VKQ52_00770"
                     /inference="COORDINATES: protein motif:HMM:NF036757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85731.1"
                     /translation="LVPLQMGFFDRFIAILYELLLSASGNIRDYYILAPLGTFMSYFY
                     QDKSRTYYVRPEMTDNGAFEFLYSDYEDLFMAILEQNTVTYNQIISTIPKNDKFFFMN
                     RFFNFYHPLVCYFMRTLFTQGIDGLMNRDTQLKGDVAYDTSTGKFDFKAYYKPTALVY
                     SGGKDPVTYPNALPNPVTDTTPGYPREDVDFDPLSGYALYNWELFFHAPLMIAERLDQ
                     NQQFEDADKWYRYIFNPTDTSPYASPDKFWVTKPFFINVNDKYTQQRIDNILLGVDAP
                     EQALVNDVTTWRNNPFQPHLIAQYRTVAYQKTTVMKYLNHLIAWGDYLFTQDTMESVN
                     EATQLYLLASQILGPKPQIIPPAYERPIYNYNQFKTLDALSNAMVDIENLMPLQTIKG
                     YDGITPQGKMPILQTLYFCLPANSQLLAYWDTIAQRLYNIRHCLNIQGVFAPLALFAP
                     PINPGLLVRAAAAGLDLGSILNDLNSPLPYYRFQTLVQKASELAGEVKSLGASLLSAL
                     EKKDAEALALLRSSQEISVQKAVMNVKTQQVTDAQDSLNNLTKQQELITIRQQYYQGL
                     INGGLSTGENVAIGLNTASTVIDAAIAIGYALSGGLKLVPNFTIGAAGFGGSPTVNAE
                     TGGSSFGNSAEDAVKTLQSIAAALDKGAALASTLAGYARRSDEWQYQLNLANKELEQI
                     AIQIAGAQVRLDIANTEVTNQQLQIDNAQATADFLNSKFTNEDLYSWMITQISTVYFQ
                     GYNLAYATAKKAEQCFRYELAIPETSYINFGYWDSLKKGLLSGELLTNDLKTMEMAYF
                     DLNQREYELTKHISLAQLDASALLMLKTTGDCWINLPETLFDLDYPGQYMRRIKSVSL
                     TIPCVTGPYTTISCTLTLNKNSVRTSSDASGTYARKMNSGVPADDPRFRDAVGSIQSI
                     AISSAQNDSGLFELNFRDERYLPFEGSGAISLWRLQLPSAIRQFDYDTISDVIVHVKY
                     TAREGGDALSLAASTNIKNAVNAMLVSQKDTGLFRLFDFRHEFPTEWYKFLNPVNPTD
                     QQVLSVNIGPERFPYFTQGYHIKITSIDLIAETPNPVSNVEMDLLSATFSPLKLLADG
                     VYGSLMHAPVASVNKTLPATDTTLKINYPMASSLPNTTPLTSANCTNLYGIVHYQLT"
     gene            3594..4157
                     /locus_tag="VKQ52_00775"
     CDS             3594..4157
                     /locus_tag="VKQ52_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLZ85732.1"
                     /translation="MKLQHPELVDLLQKAYSAEKAAAFAYQGHAASVKDKGEKAAIRQ
                     IEIDEWNHRREVLQLMQRYDVPVSRYYERRFHVVGKMISFSCHVIGWFMPFYFAGRLE
                     SGNVCEYFRMRQYFNSLGIKEHDKLLYDMGMKEKEHEIYFLGKIKTNKLLPFFERLFN
                     WGKKRSYNNIDLDKKYPVDESYEYCRK"
     gene            complement(4200..6128)
                     /locus_tag="VKQ52_00780"
     CDS             complement(4200..6128)
                     /locus_tag="VKQ52_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HLZ85733.1"
                     /translation="MDYFKRLLDLLKTEKDEDKQSYQALTAALSVSDRRANGLTWYPI
                     AIKDTEMSLGDYLIVELERTTHQEVAHSFRSGVSVMLFSNHDPAGDKLEGTVSYQSGN
                     MMKVALRTDELPDWSRDGKLGIDLLFDDNSYDEMQAALKLASSLREKSEEGRLIRVLT
                     GEQSPTSGDDQGAAKQISARLNPSQQAAVSKILSANELAIVHGPPGTGKTTTLVQAIK
                     ALIDRDHEQILVVAPSNTAVDLLSEKLSDEGLNVLRVGNPARVSERLLSLTLDSKMMA
                     HGSMKEVKKLRKRASEFRDMAQKYKRNFGPAEREQRKALFGEARSIIKEADRTEQFIT
                     DDLVSRAQVITATLVGANHYTIRGLRFGTVVIDEAGQALEPACWIPILKARKVIFAGD
                     HCQLPPTIKSAEAARGGLSTTLLEKCAALHPQSVVLLEEQYRMNEMIMGYSSSVFYDD
                     KLKAHGSVAHHSLFPGDKPLVFVDTAGCGYEERAEGISTGNPEEAAFLFRHLTRLVAE
                     IAEQGNGSGSREQGNFPSIAVISPYRLQIDLLKEQLRHSPTLQPYLDKISVNTIDSFQ
                     GQERDIVYIGMTRSNPDNKIGFLSDIRRMNVAMTRARKKLVVIGDSATLSQLPFYADF
                     ISYAEKHDAYQSAWEFVE"
     gene            6254..>8128
                     /locus_tag="VKQ52_00785"
     CDS             6254..>8128
                     /locus_tag="VKQ52_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ85734.1"
                     /translation="MARSSQVQVMGKRKLFFLGLLSLYASISAYPQATAPETTGSGAT
                     GISGTVKDAGDARPLGGVSIEVTGTKKGAVSNDDGRFSIRATAGDVLVFKMIGYEARQ
                     YKVTGPTGNLIITLRQDAKGLKDVVIIGYQTLARRNVTAAVTSIDPKAIADVPTPTLD
                     GLLQGRVAGLNVQNFSGEPGVRSSVVLRGNTSVSRAINNDATSPAGKASLARAISGPL
                     FVVDGVPQTTDDIAAINYGNGTNTDVLAGIPITDIESIDILKDASAAAIYGSRGANGV
                     IIIKTKRGIPGKPKVSLSTYHGFTERPTLDKVLIGAEETRAKMDLLQHYGGYNDLRNI
                     PMILTDTLNPAFNNANKYEDGVFQNGLVDNYSVSVTGGSEGVNYRYGLNYYNENGIIK
                     KSGLKRYSFNSNLGVRISPRLNISTQIRYYRLNRPRSISDITGGYTPFNGGYYASSRL
                     PSSTLYLSPESQAFIFGNANNQTDANVNNSLTISPNIEWRISDKWVFNTVISYEIANS
                     RRDAYTPGKYRLSGVGYASSFTDNSYNYLMSNTLQYTTSLAKYHHLNFLLGQNTEFHQ
                     YRATDAEADGIPNDQVSVVNVLNRNNSTAYSDLIESGIQSGFFRVNYDYKGRYLLSGV
                     F"
BASE COUNT         2010 a         2365 c         1930 g         1823 t
ORIGIN      
        1 acctcgtccc cctgcagatg ggattcttcg acaggtttat cgccatcctc tatgaactcc
       61 tgctttcggc aagcgggaat atccgcgatt attatatcct ggccccactt ggcacattca
      121 tgtcctattt ctatcaggac aagagccgca cctattatgt ccgccccgaa atgacggaca
      181 acggggcatt cgaattcctg tatagcgatt acgaagatct gttcatggcc atcctcgaac
      241 agaatactgt cacttacaat cagatcatca gcacgatacc gaagaatgac aagttctttt
      301 tcatgaaccg cttcttcaat ttctatcacc ccctcgtctg ttatttcatg cggacactgt
      361 tcacacaggg gatcgatgga ttgatgaaca gggacaccca gctcaagggc gatgtagctt
      421 atgataccag caccggtaaa ttcgatttca aagcctatta taagcctaca gccctcgtat
      481 acagtggcgg gaaggacccg gtcacctatc ccaacgcgct gcctaacccc gtaacggata
      541 ccaccccagg ctatcccagg gaagatgtgg acttcgatcc cctgtccggc tatgcattat
      601 ataattggga attgttcttc catgcacccc tgatgatcgc cgaaagactg gaccagaacc
      661 agcaattcga ggacgcggac aaatggtacc ggtatatatt caaccccacc gatacctcac
      721 cctatgcatc gcccgataaa ttctgggtga cgaaaccgtt cttcatcaac gtgaacgata
      781 aatataccca acaacggatc gataatatcc tgctgggcgt tgacgctccc gaacaggccc
      841 tggtcaacga cgtcaccacc tggcggaaca accccttcca gccccatctc attgcgcagt
      901 atcgtaccgt tgcctaccag aagacgacgg tgatgaaata cctcaatcac ctcatcgcct
      961 ggggagacta cctgttcaca caggatacga tggaatctgt caacgaagcg acccagcttt
     1021 acctgcttgc ttcccaaata ctgggaccaa agccgcagat catacctccc gcatacgagc
     1081 gtcccattta caattacaat cagttcaaga cgctggatgc cctgtccaat gcaatggtcg
     1141 atatagaaaa cctgatgccg ctgcagacga tcaaagggta cgatggcatt acaccacagg
     1201 gaaaaatgcc catcctgcaa acattatatt tctgcctgcc tgccaactcg cagctcctgg
     1261 cttattggga caccatcgca cagcggctat acaatatccg ccattgcctg aatatccagg
     1321 gcgtcttcgc accgctggcc ttgttcgctc caccgatcaa tcccggcctg cttgtacgcg
     1381 cggcagccgc cggtcttgat ttgggaagta tcctcaatga cctgaacagc ccgctccctt
     1441 attaccgttt ccagacactg gtccagaaag cttcggaact ggccggcgaa gtcaaatcac
     1501 ttggcgcatc gctgctgtcg gcattggaga agaaagatgc cgaagccctc gcattgctgc
     1561 ggtccagcca ggagatcagt gtgcagaaag ccgtcatgaa cgtaaagaca caacaagtaa
     1621 ctgacgccca ggattcgctg aacaacctga ccaaacaaca ggaactgatc accatccggc
     1681 agcaatacta ccagggtctg atcaatggag gtctgagcac cggtgagaat gtggcgatag
     1741 ggctcaatac tgcatccacg gtcatcgacg cagctattgc aatcggatat gcgttgtcgg
     1801 gaggcctcaa gctggtcccc aattttacga tcggagcggc aggtttcggc ggatctccga
     1861 cggtgaatgc tgaaacggga ggctccagct tcggtaattc agctgaagat gccgtcaaga
     1921 ccctgcaatc tatcgcggcc gccctcgata aaggcgccgc cctcgccagc accctggcag
     1981 ggtatgcccg tcgcagcgat gaatggcagt accagttaaa ccttgccaac aaggaactgg
     2041 aacagatcgc catccagatc gcaggcgccc aggtccggct cgacattgcc aataccgagg
     2101 tcaccaacca gcaattgcaa atcgacaatg cgcaggctac cgccgatttc ctcaatagca
     2161 aattcacgaa tgaggacctg tacagctgga tgatcacgca gatatctacc gtctacttcc
     2221 aggggtacaa cctggcctat gctactgcga agaaagcgga gcaatgcttc cgctatgagc
     2281 tggccatacc cgaaacctcc tacatcaact tcggttattg ggacagcctg aaaaagggat
     2341 tgctgagcgg ggaattattg accaatgacc tgaagacgat ggaaatggcc tattttgacc
     2401 tgaatcaacg ggaatacgaa ttgaccaaac atatctccct ggcccagctg gacgcttcgg
     2461 cactcctcat gctgaagacc accggggact gctggatcaa tttacctgaa acattattcg
     2521 acctcgacta tccgggacag tatatgcgca ggatcaaatc ggtaagtctc accataccct
     2581 gtgtgaccgg gccttacacc accatcagct gtaccctgac attgaataag aactccgtgc
     2641 ggaccagctc tgatgcctcc ggtacctatg cccgcaagat gaatagcggc gtgccggctg
     2701 acgatcccag gttcagggat gcagtcggct ccattcaatc catcgcaatc agcagtgccc
     2761 agaacgacag cggcctgttc gagctgaact tcagggatga aaggtacctg cctttcgaag
     2821 gatcaggcgc catcagcctc tggcgactgc agctcccgtc cgctatccgg caattcgatt
     2881 acgataccat cagtgacgta attgtacacg taaagtacac ggcaagggaa ggcggagacg
     2941 cgcttagcct cgctgcatct acgaatatca agaacgccgt caacgcgatg cttgtctcgc
     3001 agaaggatac gggactcttc cgtctgttcg atttccggca tgagttcccc acggaatggt
     3061 ataaattcct gaatccggtg aaccccaccg atcagcaggt actgtccgtc aatatcggcc
     3121 cggaacgctt cccttacttt acgcaaggct accatatcaa gatcacgtcc attgacctga
     3181 tcgcagagac gcccaacccg gtcagcaatg tggaaatgga cctgctgtcc gccaccttct
     3241 caccccttaa gctgctggca gatggtgtct acggcagtct catgcatgca ccggttgcct
     3301 ccgtcaacaa gacgcttccg gcaaccgata cgacgcttaa aatcaattat cccatggcca
     3361 gcagcttgcc gaataccacg ccgctgacca gcgcgaactg cacgaacctg tatggtattg
     3421 tccattacca gttgacgtga gaccctatac cggttggccc gaggcccccc ggaatatttc
     3481 cgcaaggatt tccggatcgc agcagggcag atcacaaagc agattatata aggccgccga
     3541 atttggcggc ctttgttttt agcattaagt ttatgctttc aaaacgaccc tttatgaagc
     3601 tccagcaccc ggaactcgta gacctcctgc aaaaagccta ttctgccgaa aaagccgccg
     3661 cctttgccta tcagggacat gccgcctccg taaaggacaa gggagaaaaa gccgctatcc
     3721 ggcaaatcga gattgatgaa tggaaccatc gcagggaagt attgcagctc atgcaacgct
     3781 acgacgtccc cgtttcccgg tattacgagc gcaggtttca tgtcgtcggg aaaatgattt
     3841 cctttagctg tcacgttatc gggtggttca tgccctttta ttttgccggc aggctggaaa
     3901 gcggcaatgt atgcgaatac ttccggatga ggcaatactt caattcactc gggatcaagg
     3961 aacacgacaa actgctatat gatatgggca tgaaggagaa ggagcatgaa atatatttcc
     4021 tgggtaagat caaaaccaac aagctccttc cgttcttcga acgcctcttc aactggggta
     4081 aaaagcgcag ctataacaat atcgatctgg acaagaaata tcctgtcgat gagtcttatg
     4141 agtattgcag gaaatgagct gcgtttattt ctcaagagtt ctgtcaggcc atgatctcct
     4201 cattccacaa attcccacgc actctggtat gcgtcatgtt tctcggcata actgatgaag
     4261 tccgcataga aaggcagctg tgaaagcgta gcgctgtccc cgatcaccac caacttcttg
     4321 cgggcgcgcg tcatcgccac attcatgcgg cggatatccg aaaggaagcc gatcttgtta
     4381 tccggattgc ttcgcgtcat gccgatatag acaatatcgc gctcctgccc ctggaaactg
     4441 tcgatcgtat tcacggaaat tttatccagg tatggctgaa gggtagggga gtggcgcagc
     4501 tgctccttca gcagatctat ctgcagcctg tatggagaga tcacggcgat tgacgggaaa
     4561 ttcccctgct cccggctccc ggatccattc ccctgctccg cgatctccgc aaccagcctc
     4621 gttaaatgcc tgaacaggaa ggccgcttct tccggattgc cggtactgat cccctccgcc
     4681 cgctcctcat aaccgcatcc agccgtatcc acaaatacca gcggcttgtc cccggggaac
     4741 agggaatgat gcgctaccga tccatgcgcc ttcagcttgt cgtcatagaa cactgaggaa
     4801 gaatagccca tgatcatttc attcatccgg tattgttctt ccagcaggac gaccgattgc
     4861 ggatgaaggg ccgcgcattt ttccagcagt gttgtgctca acccgccacg cgccgcttcc
     4921 gccgacttta tggtaggagg taattgacaa tggtctccgg caaagatcac cttcctggct
     4981 ttcaggatcg gtatccaaca ggcaggctca agcgcctgac cagcttcatc gatcaccacc
     5041 gtcccgaacc ggagcccccg tatcgtataa tgattggcgc ctaccagggt agccgtgatc
     5101 acctgtgccc tggataccag gtcgtcggtg ataaactgct ccgtcctgtc cgcctctttt
     5161 ataatgcttc gtgcttcccc gaaaagtgcc ttgcgctgtt cccgctccgc cggcccgaaa
     5221 ttccgtttgt atttttgcgc catatccctg aactcggatg ccctcttccg tagcttcttc
     5281 acttccttca tactcccgtg cgccatcatt ttactgtcca atgtcaatga caacaaccgc
     5341 tcggagacac gtgccggatt gcccacccga agcacattca ggccctcgtc ggacagcttc
     5401 tcgctcagca ggtccactgc cgtattactt ggcgccacca ccaggatttg ttcgtggtca
     5461 cggtcgatca gggctttgat cgcctgcacc aatgtcgttg tcttgccggt tcccggaggg
     5521 ccatgtacga tagccagctc attagcggaa aggatcttac tcaccgccgc ctgctgcgaa
     5581 ggatttagcc ttgcagaaat ttgtttcgcg gctccctggt catccccgga tgtgggcgat
     5641 tgctctcccg tcaataccct gatcagccga ccctcttcgg atttctctct gagcgaagaa
     5701 gccagcttaa gggcggcttg catctcatcg tagctgttgt catcgaacaa cagatcgata
     5761 cccagcttcc cgtccctgct ccaatcagga agctcgtccg tccgcaacgc gaccttcatc
     5821 atattcccgc tttgatagga gacggttcct tccagtttgt caccggccgg atcatgattg
     5881 gaaaataaca taacggaaac gccggaacgg aaagaatgtg cgacctcctg atgcgtagtt
     5941 cgttccagct ccacaatcag gtaatccccc aggctcatct cggtatcctt gatcgcgatg
     6001 ggataccatg tcaatccatt cgccctgcga tccgataccg atagtgccgc tgtcaatgcc
     6061 tgataagact gcttgtcctc atccttttcc gttttgagca aatccagtaa cctcttgaaa
     6121 taatccatcg gacaaaggtc ggaattttcc ctttaaagtc tgttaacaat ccgtttgaac
     6181 tctattgaca ggtttcaatt ctttgtcaat ttaacttagc catcgctatc cacgatcacc
     6241 aaaaactact gctatggcaa gatcttcaca ggtccaagta atgggcaagc ggaaattatt
     6301 tttccttggc cttctttctt tatacgcttc catcagtgca tatccgcagg cgacagcgcc
     6361 agagacgaca ggctccggcg ccaccggtat atccggcacc gtcaaagatg ccggtgatgc
     6421 caggccgctt ggcggtgtat ccatcgaggt tactggcacc aaaaaagggg ctgtctccaa
     6481 cgacgacggg agattctcca tcagggctac cgccggcgat gtgctcgttt ttaaaatgat
     6541 cggatatgaa gcgaggcaat acaaagtaac cggtcccacg ggcaacctga tcatcaccct
     6601 tagacaggat gcaaaagggc tgaaggacgt cgtcatcatc ggctatcaga cgctggcccg
     6661 ccgcaacgta actgcggcag ttacctcgat cgaccccaag gcgatcgcgg acgtgccgac
     6721 tcctacattg gacggactgc tgcaaggtcg tgtagccggc ctgaatgttc agaacttttc
     6781 cggcgaaccg ggtgtcagaa gttccgtcgt gctcagggga aatacctcgg tcagcagggc
     6841 catcaataat gacgcgacct ctccagcagg caaggccagc ctggccaggg cgatcagcgg
     6901 tcccctgttc gtggtagacg gcgttcccca gaccacggac gatatcgcag ccatcaatta
     6961 tggcaacggg accaatacgg atgtgctggc cggcatcccc atcaccgata tcgagagcat
     7021 agacatcctg aaggatgcct ccgccgccgc tatttacggg tccaggggag ccaatggcgt
     7081 catcatcatc aagaccaaaa gaggcatccc cggcaagccc aaggttagtc tgagtaccta
     7141 tcacggtttt accgagcggc cgacattgga taaagtgctc ataggagcgg aagagacccg
     7201 tgcaaagatg gacctgcttc agcattatgg cggatataac gatctccgga atatccccat
     7261 gatcctgacc gataccctta acccggcctt taacaacgcc aataaatatg aggacggcgt
     7321 cttccagaac ggattggtgg ataattacag cgtctccgtt acaggcggca gcgagggggt
     7381 caactatcgt tacggattga attattacaa tgagaacggc atcatcaaaa agtcgggcct
     7441 gaagcgatat agcttcaaca gtaacctggg cgtcaggata tccccccgtc tgaatatcag
     7501 cacgcagatc aggtattacc ggctcaaccg gccccggtcc atcagcgaca tcacgggagg
     7561 atatacccct tttaacggag gatattatgc ttccagccgc ctgccgtcct ccacactgta
     7621 cctgtcgcct gaaagccagg cgttcatatt cgggaacgca aacaatcaga ccgacgccaa
     7681 tgtgaataac agcctcacca tcagccccaa catcgaatgg cggatctccg ataaatgggt
     7741 attcaatacc gtgatctcct atgaaatagc caatagcagg agagatgcct atactcccgg
     7801 caaataccgc ctgagcggag tagggtatgc gagcagcttc accgataatt cctacaatta
     7861 cctgatgagc aataccctcc aatacactac cagcctggca aagtaccacc acttgaattt
     7921 cctgctgggc cagaacacgg aatttcacca ataccgcgcc acggacgcgg aagccgatgg
     7981 cattcccaat gaccaggtca gcgtcgtcaa cgtattgaac aggaacaact ccaccgctta
     8041 ttccgacctg atcgaaagcg gcatccaatc cggcttcttc cgggtcaact atgattacaa
     8101 gggacgttat ctcctttccg gcgtattc
//
LOCUS       DATGFG010000041         3944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_906607, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000041 DATGFG010000000
VERSION     DATGFG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906607"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VKQ52_00790"
     CDS             <1..492
                     /locus_tag="VKQ52_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85735.1"
                     /translation="YEIKNAQAMKIMDCLGAGGSFTEYYAMDFDADVVLMGHDGPGHI
                     DIAEGRTIVRPLQVYHGKVGSGLSVEMSVRKGPVTLLSVVEKKGGGLFLLMAEGESVD
                     GPILQIGNTNSRYKFPIGARNFVHRWNSYGPAHHCAVGVGHLGSRLEKFAALIDMEII
                     KVC"
     gene            520..1407
                     /locus_tag="VKQ52_00795"
     CDS             520..1407
                     /locus_tag="VKQ52_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ85736.1"
                     /translation="MINYYRYLPISEEDQHWGLSILNAGSTRIAADQEYPYRQHPSDH
                     YFTWNKGRVLDEYQVIYIVGGQGSFESTHIPETVVHSGTVILLFPGEWHRFRPAKGTG
                     WDEYWVGFRGEVADNLVKRKFFEASAALLPIGFSAELAGLFSAILDQTRTEQPGYQPV
                     ASGILLHLLGCVFSLNKQRSFEHAEEIRNRMEHAIMLLRDKIDEDIAIRDIAAQLNVS
                     YAWFRKWFKLYTGLAPHQYVIQLKIEKAKALLADSGNSIKGIAYQLRFEHPLYFSKLF
                     KEKVGVSPEGYRKNCLTRP"
     gene            1540..3006
                     /locus_tag="VKQ52_00800"
     CDS             1540..3006
                     /locus_tag="VKQ52_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85737.1"
                     /translation="MRRILTLTFLLIPFLSFSQSVEKNVYDPGDPTNGYYLAVRPRSG
                     SIRGTLLLLTSFTPPEALLSETRLHNVTYANDLLTVYVSLGKKICADTATINRINLMV
                     KHLTTTYSADTSRMALAGYSYAGGVALRYTELAYKNPSVCGFQPKAVFAIDAPVDLIG
                     LWHWCEREIKKNYFPGSVADGKILLDLMTKDIGAFPATPDTYQYLTPFNKDLVSSGNE
                     QSLVSIPLRLYYDADIQWQLANRRNSLYDTNIPDGSELINRLLLLGNARAEFVSAKMP
                     GVRSNGMRNPNSLSIVDEVDCIQWIRKALGIFDPNTWVPPYQYSAPAGWSIERFALPP
                     DFAPQITWKGVEDVRFHPGWGDSLSEGYWSYTYLWWIDGNPVIDAASLQQALQAYYTG
                     LVNRNIVPGKIPADKRIPTVATIKKIKPAEKDKETYTGVIRMLDYLAQRPMELNTIIH
                     VRVPQSSGKTVVLVGISPQPFSHPVWKTLDKIPADFAP"
     gene            3446..>3944
                     /locus_tag="VKQ52_00805"
     CDS             3446..>3944
                     /locus_tag="VKQ52_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLZ85738.1"
                     /translation="MENAKKPPFITGPQDGRNVSVVGDTYRILITGKETGGAFATIDM
                     LIPPGGGPGPHAHPNFHESFFVLDGEIEVKSEAGTYTARKGSFVHIPEGGIVHCFKNK
                     TDQTAHLLCIVVPSGLEEFFMEIGKPVAVGSFLAPPPMDEEAMKKILPIVEKYGQKLF
                     PPDYLG"
BASE COUNT          972 a         1072 c         1019 g          881 t
ORIGIN      
        1 tatgagataa agaatgcgca ggcgatgaag atcatggatt gtctgggtgc cggagggtct
       61 ttcaccgagt actatgccat ggatttcgac gccgatgtcg tattgatggg gcatgacggg
      121 ccggggcata tcgacattgc cgaaggccgg acgatcgtcc ggccgctgca ggtatatcat
      181 ggaaaagtgg gcagcgggct gtcggtggag atgtcggtga ggaaggggcc ggtgacgctg
      241 ctatccgtag tcgaaaaaaa ggggggcggt ttgttcctgc tgatggccga aggggaatcg
      301 gtagacggac caattttaca gatcggcaat accaatagcc ggtacaaatt cccgatagga
      361 gcaaggaatt tcgtccaccg ctggaatagc tacggccctg cccaccactg tgcggtcgga
      421 gtcgggcatc tgggctcccg gctggaaaag tttgcggcgt tgatagatat ggaaataata
      481 aaagtatgtt agatttaagg tccaaatgcg gcagcaacca tgatcaacta ctaccgatat
      541 ctgccaatca gcgaagagga tcaacactgg ggcttgtcga tcttgaatgc cggcagcacg
      601 cggatagccg ccgaccagga atatccctac cggcagcacc caagcgacca ttatttcacc
      661 tggaataagg gccgggtatt ggatgagtac caggtcatct acattgtcgg aggccagggt
      721 tcgttcgaat ccacccatat tccggagacg gtagtccatt ctggaacggt catcctgctt
      781 ttccccgggg aatggcaccg tttccggccg gccaaaggaa cgggctggga cgaatactgg
      841 gtcgggttca ggggtgaagt agcggacaac cttgtcaaac gaaaattttt tgaagcgtcc
      901 gcggcgttgc ttcccatcgg gttcagcgcg gagctcgcgg gactgttctc agcgatcctc
      961 gaccaaaccc gtacggagca gcccggctat caaccggttg cttcgggcat cctgcttcat
     1021 ctgctgggct gtgtcttttc tctgaacaaa cagcgatcct ttgagcacgc ggaggagata
     1081 aggaacagga tggaacacgc aatcatgctg ctgcgtgata agatcgacga agacatcgcc
     1141 atccgggata tagcggcgca actgaatgtc agctatgcct ggtttcgaaa atggttcaag
     1201 ctgtataccg gcctggcgcc gcatcaatat gtgatccagc tgaagatcga aaaagcaaaa
     1261 gccctgcttg ctgattccgg caattccatc aaagggatag cgtaccagct ccggttcgaa
     1321 catcccttgt atttctcaaa gctcttcaag gaaaaggtcg gtgtctcgcc ggagggctat
     1381 agaaagaatt gcctgacgcg gccttagaat cgaacaccga ttgtatcgaa accgcgcaaa
     1441 cagacacctt ggtcaagatt aacattttgg ttatcaacta tttaataaca ggcacattcc
     1501 ttgggcataa aagccgttct tcatctcaaa aatgagccta tgcgtcgaat ccttacactg
     1561 acctttcttt tgatcccctt cctctctttc tcgcaaagcg tggaaaagaa cgtgtatgac
     1621 cccggcgacc ccacgaatgg ctactacctc gcggtccgcc ctcgttccgg cagcataaga
     1681 ggtaccctgc tcctgctcac cagttttacc ccgccggagg cgctcttatc ggaaacacgc
     1741 ttgcacaacg tcacttatgc caacgatttg ctcaccgtat atgtatcgtt aggaaagaaa
     1801 atatgcgctg atacggcgac gatcaaccgc atcaacctca tggtgaagca tcttacaacc
     1861 acctattccg ccgacacttc ccggatggca ctggccgggt actcctatgc cggcggtgtt
     1921 gccctgcgct atacagaatt ggcgtataaa aacccttccg tatgcgggtt tcagccaaaa
     1981 gcggtctttg cgatagacgc gcccgttgac ctcatcggcc tctggcattg gtgcgaaagg
     2041 gaaataaaga aaaactactt cccgggttcg gtggcagatg gaaagatttt gctcgacctc
     2101 atgacaaagg atataggcgc gttccccgca acccctgaca cctatcaata tttgacgcct
     2161 ttcaacaagg atcttgtctc ctccggcaat gaacaaagcc tcgtaagtat accattaaga
     2221 ctgtactacg atgccgatat ccaatggcag ctcgccaacc gccggaacag tctgtacgac
     2281 accaatatcc ccgatgggtc ggaactgatc aaccgcctct tgctccttgg caacgcccgg
     2341 gcggaatttg tctcagccaa aatgccgggc gtccgtagca atggcatgcg caatcccaat
     2401 tcgctctcga tcgtcgatga agtggactgt atccaatgga tcaggaaggc attgggcatt
     2461 ttcgatccca atacctgggt gcccccttac cagtattccg cgccggccgg gtggtcgatc
     2521 gagcgctttg cgctgccccc cgacttcgcg ccgcagatca cctggaaagg tgtcgaggat
     2581 gtccgttttc atccggggtg gggcgactcg ctgagcgaag gctactggtc ctacacctac
     2641 ctgtggtgga tagacggcaa ccccgtgatc gacgcggcga gcctccaaca ggcactccag
     2701 gcctattaca cgggcctcgt gaacaggaac attgttcctg ggaagatccc ggcggacaaa
     2761 cgcattccca ccgttgccac tatcaaaaag ataaagccgg cagagaagga taaagaaacc
     2821 tacaccggcg ttattcgcat gctggactac ctggcccaac gtccgatgga attgaacacc
     2881 atcatccacg taagggtgcc tcagtcatcc gggaaaacgg ttgtcctggt cgggatctcc
     2941 ccgcagcctt ttagccaccc ggtttggaaa acgctggaca agatcccggc ggacttcgcg
     3001 ccataagttc ccattacggt ccctccccgc tttttctggg tgaaaactga tagggaatac
     3061 ttaatttcgt gcaactatgg taaaaaaatc agccaaatcc ccaaaagtaa aactggcaaa
     3121 gaagaaaaca acaacaagga aagcggtcaa acccgcgaaa gacgccagcc tcgtaaacga
     3181 acttaggttg gcggctaaag cttctgctat caggcggctc aataaatagc atgatcatct
     3241 gttgctcatc ggatcctttc ttcatagcta tttggcccct gacttagcag aaagctggtt
     3301 tgtctcttcg gtattgggtt tgtagacgcc caggcctccc tgtatttcct cctccaggcc
     3361 gatcggacta tttttacgcc taacccggtt atttttcctg gtcccattgc gtgaattttg
     3421 acttacataa atcaattcac gcatcatgga aaatgcaaaa aaacctccct ttataaccgg
     3481 cccccaggat gggcggaatg tttctgtggt cggggacacc tatcgcattc ttatcaccgg
     3541 caaggaaacc ggtggtgcat tcgctacgat cgacatgctc ataccacccg gtggcggccc
     3601 cggaccacac gcgcatccaa actttcacga atcctttttt gtgctcgacg gcgagataga
     3661 ggtaaagtcc gaagcgggaa cctacaccgc caggaaggga tcattcgtcc acatccccga
     3721 gggagggatc gtgcattgct tcaaaaacaa aacggaccag acggcccacc ttctttgtat
     3781 agtcgttccc tccggcctgg aggaattttt tatggagata ggtaaacccg tggccgtcgg
     3841 atctttcctc gcacccccgc cgatggatga agaggctatg aagaaaatac tgccgatcgt
     3901 cgagaagtac gggcagaaac tctttccccc ggactacctg ggat
//
LOCUS       DATGFG010000042         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1131270, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000042 DATGFG010000000
VERSION     DATGFG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131270"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VKQ52_00810"
     CDS             complement(<1..676)
                     /locus_tag="VKQ52_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HLZ85739.1"
                     /translation="MTNSKPTLYTALSPALLHLYDLNHAVVVIIDVLRATSTIAAALH
                     NGAKCVIPVDSVAKCIELGRQIDGITAGERDGMIAEGLEYGNSPFEYPREFIYNRTLV
                     LTTTNGTKLLHMALERGAGQIITGSFPNLRSVCDYLIAQNQNVVLGCAAWKDRINLED
                     MLFAGAVIHQVKNHFSINCDSSQIAESVYQEAKKDLFGFMQSKDASHYHRLMGFGLEK
                     DIRYCLT"
     gene            complement(740..1216)
                     /locus_tag="VKQ52_00815"
     CDS             complement(740..1216)
                     /locus_tag="VKQ52_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLZ85740.1"
                     /translation="MPTIHLTTFVAAPADRVFDLARSIDLHRKSMMHTDEQAIAGTTS
                     GLIGADETVTWKAKHLMKTRILKSKITSMDRPLSFTDEMVDGDFKSLRHEHHFKRIDN
                     GTLLIDLFHFETPYGGLGKLVNELFLTRYMKKLLEIRNQMIKEYAESEKWKFILDK"
     gene            complement(1332..>2258)
                     /gene="gcvT"
                     /locus_tag="VKQ52_00820"
     CDS             complement(1332..>2258)
                     /gene="gcvT"
                     /locus_tag="VKQ52_00820"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HLZ85741.1"
                     /translation="FIVKGEHALDLIQRVTTNDAAKLTNGKAQYSALTNEKGGIVDDL
                     IVYCLEENKAYMLVVNASNIEKDWKWISSHNTKHAELHNISDKTGLLAIQGPNATRIM
                     QPLTDIDILNLPYYTFAKGKFAGVDNVLISATGYTGAGGIEIYFEDKDGAAETIWNAI
                     FEVGAPQGLKPIGLGARDTLRLEMGYCLYGNDIDDTTSPLDAGLGWITKFTKDFTARP
                     ILETQKAAGPQQKLVGFEMLEKGIPRHDYEIKDFSGMVIGRVTSGTQSPSLGKAIGMG
                     YVRTAFSHIDTKIYIKVRDKLLQARVVKLPFN"
BASE COUNT          444 a          526 c          717 g          571 t
ORIGIN      
        1 gtgtgaggca gtagcggata tccttttcca gcccgaatcc catgaggcgg tgataatgtg
       61 aggcatcctt gctctgcata aacccaaaaa gatctttttt ggcctcctgg tagacggact
      121 cggcgatctg ggaggagtcg cagttgatgg agaaatggtt tttcacctgg tggatcacgg
      181 cgcccgcgaa gagcatgtct tcaaggttta tccggtcttt ccaggccgcg cagccgagca
      241 caacgttctg gttctgagcg atcaggtagt cgcagacgga ccggaggttg gggaaggagc
      301 cggtgatgat ctggccggcg ccccgctcga gggccatgtg cagcagcttt gtaccgttgg
      361 tggtggtgag aacgagggtc cggttgtaga tgaactccct ggggtattcg aagggggagt
      421 taccgtattc gaggccttcg gcgatcatcc cgtcccgttc gccggcggtg atgccgtcta
      481 tctggcggcc cagctcgatg catttggcga cgctgtccac cgggatcacg catttggccc
      541 cgttatgaag ggcggcggcg atggttgaag tggcgcggag gacgtcgatg atcaccacaa
      601 cggcatggtt cagatcgtat aaatgcaaca gggctgggga taaagccgta tataaagtcg
      661 gtttggaatt tgtcataata tgtgacggac gggcggtccg ttataggcga taaacgcaaa
      721 aatagggctt cgggcgcgtt tatttatcca aaatgaactt ccatttttcc gattcagcgt
      781 attccttgat catttgatta cggatctcca gcaatttttt catgtagcgg gtgaggaaaa
      841 gttcgtttac gagcttcccc aggccgccat agggtgtctc gaaatggaag agatcgatca
      901 gcagggtgcc gttgtcgatg cgtttgaagt gatgttcgtg ccggaggctt ttgaagtcgc
      961 cgtctaccat ttcgtcggtg aaggagagcg gccggtccat ggaagtgatc ttcgatttca
     1021 ggatgcgggt cttcatcagg tgtttcgcct tccaggtgac ggtttcgtcg gcgccgatga
     1081 gcccggaggt ggtgccggcg atcgcctgtt cgtcggtatg catcattgac ttacggtgga
     1141 gatcgatgct acgggcaaga tcaaagaccc ggtcggcggg agctgcgaca aaggtcgtca
     1201 gatgaatagt gggcatggcg tggcttgtta taaggcgttg gataatgcga caaggtagaa
     1261 tgccctttat gcaagaaaaa atcattccaa aaccggggga gccggtcggg gaatggccgg
     1321 gaccgaaaga tttagttgaa cgggagcttt actaccctgg cttgcaggag tttgtcccgg
     1381 accttgatat agatctttgt gtcaatgtgc gaaaaagctg tccggacata gcccatgccg
     1441 atggccttac cgagagaggg gctttgggtt ccggaggtta cccggccgat gaccatgccg
     1501 gagaagtcct tgatctcgta atcgtggcgg gggatgcctt tttcgagcat ttcgaaaccg
     1561 accagttttt gctggggtcc ggcggccttc tgtgtctcga ggatgggcct ggcggtgaaa
     1621 tcttttgtga atttggtgat ccagcccagc ccggcatcca ggggagaggt ggtatcgtcg
     1681 atgtcgttgc cgtagagaca atagcccatt tccagccgca gggtatcacg ggctccgagg
     1741 ccgatcggtt tcaggccctg gggggcgccc acttcgaaga tggcgttcca gatcgtttcc
     1801 gcggcgccgt ccttgtcctc gaaatagatc tcgatgcccc cggctccggt gtatcccgtg
     1861 gcgctgatca ggacattgtc cacgccggcg aatttaccct ttgcgaaagt atagtaggga
     1921 agattgagga tgtcgatatc ggtcaggggc tgcatgatac gggtggcgtt gggtccctgg
     1981 atggccagca ggccggtctt atcggagata ttgtgcagct cggcgtgttt tgtgttgtgg
     2041 gaggagatcc atttccagtc tttttcgata ttggaggcat tgacgacgag catataggcc
     2101 ttgttctctt ccaggcaata gacgatcagg tcgtctacga taccgccttt ttcgtttgtc
     2161 agggcgctgt actgggcctt tccatttgtg agttttgccg cgtcgttggt cgttacccgc
     2221 tggatcaggt cgagggcatg ttctcctttt acgatgaa
//
LOCUS       DATGFG010000043         9068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_338315, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000043 DATGFG010000000
VERSION     DATGFG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338315"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VKQ52_00825"
     CDS             complement(<1..183)
                     /locus_tag="VKQ52_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85742.1"
                     /translation="MKFRNFKMVGYVVYGRGSFNQLDEILAPQRKGDAPIIFLLDHYF
                     ENKPLARRIPLRGKDKL"
     gene            complement(240..1175)
                     /locus_tag="VKQ52_00830"
     CDS             complement(240..1175)
                     /locus_tag="VKQ52_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLZ85743.1"
                     /translation="MNQPIWGIDLGGTKIEGVILKSITDPTPIVRTRVDTEASKAYEH
                     IVSQIARLVAEMGSQSGLKPGAIGIGTPGVLDPLLQTMKNCNTVVLNGKPLKKDVEEV
                     LLLKTELANDANCFALAEAQWGIVRQSAPEARLVFGIIMGTGVGGGIVMDGKIWNGRH
                     GIGGEWGHNFLDESGGPCYCGKTGCVETVISGPATERFYKSLTGQPLKLKEIVARNRE
                     GSDPAATKTIERLCHFFGKGVSVITNLLDPDVIVVGGGVGNIDALYSGGLRELGRFIF
                     NNRVDVPLLKPSLGDSAGVFGAAALVADLHPVYPA"
     gene            complement(1288..2013)
                     /gene="kdsB"
                     /locus_tag="VKQ52_00835"
     CDS             complement(1288..2013)
                     /gene="kdsB"
                     /locus_tag="VKQ52_00835"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HLZ85744.1"
                     /translation="MKIIAMIPARYAATRFPAKLMQPLGEKTVIRHTYDNTRATGLFD
                     EVMVVTDSDIIYEEITRHGGKAKRSIRPHESGSDRIAEAVADMDVDIVVNVQGDEPFV
                     RREPLEQLLEVFRGEAGREVQVGSLVQVLREQRFIDDPNYVKVVLDKNNNALFFSRSV
                     VPYPRDPSAAVIYYEHIGVYAFRKQALLQFTAWPVSPLEAVEKIECLRYLENGVPLRM
                     VVTTYMGVEIDTPEDLIRAAKLL"
     gene            2126..3574
                     /locus_tag="VKQ52_00840"
     CDS             2126..3574
                     /locus_tag="VKQ52_00840"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HLZ85745.1"
                     /translation="MQTNELLNSYDVGAQTWDEMYAHQAVRTQYQGVVDFLQQMSIEE
                     LNKKEELAKRLFMSQGITFTVYSSGEGIEKIFPFDIIPRIITATEWNYIEAGIRQRLK
                     ALNLFLKDVYNNQFILKDGIVPVEMVYSCPHFLREMHGFPIPYDIYVHIAGIDLIRDQ
                     DGTFYILEDNLRTPSGVSYMLENREITKRIFPDLIPKNNVRTVTEYPNILHRNLVSLS
                     PRQTANPTIVLLTPGIYNSAYFEHTTLARLMGVELVEGRDLVVDNHRVYMKTTAGLQQ
                     VDVIYRRVDDGFLDPLVFQPDSMLGVSGIMSAYRKGNVAIDNAVGNGVADDKAIYAFV
                     PAMIRYYLNEEPILKNVPTYQLGNPEEREYVFANMNRMVVKKTNESGGYGMLMGHSAE
                     EEEIMRYKTEILKDPRQYIAQPTINLSSAPCYMNGRLKPRRVDLRPYALCGPDGIQIV
                     PGGLTRVALREGSLVVNSSQGGGSKDTWVLAG"
     gene            3610..4542
                     /locus_tag="VKQ52_00845"
     CDS             3610..4542
                     /locus_tag="VKQ52_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HLZ85746.1"
                     /translation="MLSRVADSLYWMSRYMERTDGILRMLKINYASSQDDIQEFSWKP
                     VLRIFSYLEEEQATSIAHQTRTVLQYMVTSKENTNSVLNIVTRARENARSVQDHITKE
                     LWQCLNDFYHTIRDEQLVRGLQKDDPVSILDILIRQGLLYYGITDITMARGEGYAFIN
                     IGKFLERGVQSADILDIKFSDLQYDLTHTDTTYWKYLLLSISGYELYLKTYRSGFDAR
                     NVVEQIVLNEDFPRSVIYSINQLQRYFERLKSDRNKDAYHKIDFMIGRIRSRVKYSTV
                     ETILRQGLHSYLNGVKEELYEVGNALNQHYFAYT"
     gene            4586..5551
                     /locus_tag="VKQ52_00850"
     CDS             4586..5551
                     /locus_tag="VKQ52_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HLZ85747.1"
                     /translation="MPRFNIHHITKYTYEGPVRDSANQIVLFPIKDEFQEILRHDLTV
                     TGEPVVEVYKDYYGNEVGSFALAEPHSSLIIDSRVEVITKQRPPLRDQAEKEAQWALL
                     EQIRWQVPYIDFLKQEQLASLSEVLESICPGKGRNQTPLEAAQQLRTYVYDQFKYIKG
                     VTSVESTLDEVWELKAGVCQDFAHMLLVFLRQIQIPARYVSGYICPNQNGMRGEGATH
                     AWIEAYIPFNGWVGLDPTNNCVTDDLHVRLAVGRSFSDCSPVRGTYKGTAGQMLEVGV
                     SVSYENGRTSEDAVTVLAPQPTVNGKDINSYRRYMEMMQQQQQQQ"
     gene            complement(5496..6386)
                     /locus_tag="VKQ52_00855"
     CDS             complement(5496..6386)
                     /locus_tag="VKQ52_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HLZ85748.1"
                     /translation="MESLVYYFQHIPSYQRSLILAGGLLFFWIIEGIVPMVRFRYHRV
                     RHAGLNLFFTLTTVIVNFAFASLIIRAADYSTAHHTGLLYLLPLPLWLFTLLGLLILD
                     FIGAWLIHWLHHQVKWLWKFHLVHHSDNWVDTTTANRHHPGESFFRAFFTLIAVIVAG
                     TPVWLVFLYQSLSALFSQFNHANLALPKWLDNVLAWVIVSPDMHKVHHHYRRPLTDTN
                     YGNIFSIWDRLFGTYRFVRDTKGLVYGIDTHPGEAEHNRMGALLAMPFQAYRPPTEEK
                     EETTVVVAAAASSPYTGDKN"
     gene            complement(6422..7537)
                     /gene="dnaN"
                     /locus_tag="VKQ52_00860"
     CDS             complement(6422..7537)
                     /gene="dnaN"
                     /locus_tag="VKQ52_00860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HLZ85749.1"
                     /translation="MKFIVSSSALLKQLQQISGVINANTVLPILEDFLFEVEKNKLTV
                     VATDLETVMKVHLEVEAKDTGKVCIPAKILIDSLKNIPDQPLTFNIDKHFAVELTSDN
                     GKYKVMGENPDNFPKEPASDETTSFTTTSSALVTAINKTLFAVSNDDLRPAMTGVFFE
                     MDKKGLQFVATDAHRLVRYKRKDVSCPKSDSLIVPKKPLNLLKSALPSNEDEITISYN
                     SNHLFVSHGTTQMSCRLIDARFPDYKVVIPADNPYKLVVGRNDFQSALRRVSIFSNKS
                     TNQVVLNIQGSELQLAAQDVDFSFEGTERMKCQYDGEDLAIAFNARFLIEMLASTDSD
                     EVRMELSTPTKAGIIKPTEEDENEEVLMLVMPLMLNS"
     gene            7673..8146
                     /locus_tag="VKQ52_00865"
     CDS             7673..8146
                     /locus_tag="VKQ52_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HLZ85750.1"
                     /translation="MRRTTLTPGQALQKARHYCGYQERCHQEVQEKLYSFGLRKPEVD
                     QILATLIEENYLNEERFALQYAGGHFRLKRWGRVRIRYQLKQKQVSEYCIKKALASID
                     QEEYEQTLLRLAEEKTQSLAVETNPFARKQKLQEYLLRKGYEPDRVAAVVGSAAQ"
     gene            8179..8988
                     /locus_tag="VKQ52_00870"
     CDS             8179..8988
                     /locus_tag="VKQ52_00870"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLZ85751.1"
                     /translation="MSSLTITTIQPDLVWEDKTANLQRLEKQILSIPDNTEIVVLPEM
                     FSTGFSMRPEALAESMEGPTLNWMRGIAARRKIILTGSVIIEEGGQYFNRLIWMLPNG
                     QFGYYDKRHRFAYAGENEHYTAGQKRLVASVKGWRVLLLVCYDLRFPVWSRQTPQDPE
                     APADSPLEYDLLLFVANWPERRSLAWKTLLQARAIENQAFVVGVNRVGNDGNNIYHSG
                     DSLIIDPLGEILYHGFGKEEVVTHTLQKERLEEVRQRFPFWRDADHFSIEP"
BASE COUNT         2217 a         2632 c         2341 g         1878 t
ORIGIN      
        1 gagcttgtct tttccccgca gcgggatccg gcgggcgagg ggcttattct cgaaatagtg
       61 gtccagcagg aagatgatcg gggcgtcgcc ctttcgctgg ggggccagga tctcgtccaa
      121 ctgattaaaa cttccccggc catatacgac atagccaacc atcttgaaat tccgaaactt
      181 catagtatat caaattgacc cgcaaaaata aggtatgttg atcaaaagcc cgccccagat
      241 caggcaggat acacaggatg caagtccgcc accagcgcgg cggccccgaa aacgccggcg
      301 ctatccccca gggaaggttt tagcagcggc acatccaccc ggttgttgaa gatgaaccgg
      361 cccagctccc gcagcccccc cgaatacagg gcgtcgatat tgccgacacc gccaccgacg
      421 acgatgacat cggggtcgag caggttggta ataacggaaa cgcccttgcc gaagaaatga
      481 caaaggcgct cgattgtctt tgtcgccgcc ggatcactgc cttcgcggtt ccgggcgacg
      541 atctctttta attttagcgg ttgcccggtg agactcttgt aaaagcgctc ggtggccgga
      601 ccggaaatga ccgtctcgac acaacccgtc ttgccgcaat aacagggacc acccgattca
      661 tccaggaaat tatgccccca ttccccgcca atgccatgac ggccgttcca gatctttcca
      721 tccatgacga tccctccgcc tacccccgtc cccatgatga tcccgaaaac aagccgcgct
      781 tccggtgcgg actggcgaac gataccccac tgggcttccg caagggcaaa gcagttggcg
      841 tcattggcta actccgtctt cagaagcagg acctcttcga catccttttt caggggcttt
      901 ccgttcagca caaccgtatt acagtttttc atggtctgca aaagaggatc aaggacaccc
      961 ggagtgccga tgccgatggc gccgggcttt agccccgact ggcttcccat ttcggcgaca
     1021 agccgggcta tctggctgac gatatgctca tatgccttgg atgcttccgt gtctactcgt
     1081 gtccggacga tgggagtagg gtcggtgatg gatttcaaaa tgaccccttc gatcttggtt
     1141 ccgcccaggt caattcccca gattggttga ttcatgttgc aggtagttct tgccaaaaaa
     1201 aaatcttcgc ggctaattta aggattcccc tttttagcat tttggataat ttttttttgc
     1261 aggacgcggg ccaatttttt gcaggaccta gagcaattta gcagcccgga tcaggtcttc
     1321 cggggtgtcg atctcgacgc ccatatacgt ggtaaccacc atccgcaggg gaacgccgtt
     1381 ctccaggtaa cgaagacact cgatcttctc caccgcctcc agcgggctca ccggccacgc
     1441 cgtgaactgc agcaaggcct gtttgcgaaa agcgtatacg cctatatgtt cataatagat
     1501 gacggctgcg gaagggtcgc gtggataggg gactacactg cggctaaaga acagggcgtt
     1561 attattctta tccagcacaa cctttacata attggggtcg tcgatgaacc gctgctcccg
     1621 caacacctgt acaagcgacc ccacctgcac ctcccggccc gcctccccac ggaatacctc
     1681 cagcagctgt tcgaggggct cccggcggac aaagggttca tctccctgga cattgacgac
     1741 gatatcgaca tccatatccg caacggcctc cgcgatccgg tcgctcccgc tctcatgcgg
     1801 ccggatgctc cgcttcgcct tcccgccatg acgggtgatc tcttcataaa tgatatcgct
     1861 gtccgtcacc accatcacct catcgaaaag acccgtggcc ctggtattgt cataagtatg
     1921 ccggatcacg gtcttttccc ccaggggctg cataagcttg gcgggaaaac gggtcgccgc
     1981 atagcgggcg gggatcatcg cgattatttt catggggacg aaaataacat tttctccttt
     2041 tttatatata tttagataac taacgcccgc ctgccaggaa ttttgcctcg aggacacaaa
     2101 cccagcacaa aaccaagact agcttatgca aaccaatgaa ctgctcaaca gctatgacgt
     2161 gggcgcccag acctgggacg agatgtatgc ccatcaggcc gttcgcaccc aataccaggg
     2221 cgtcgtggat ttcctgcaac agatgagcat cgaagagctc aacaaaaaag aagaactggc
     2281 taaacggctc tttatgagcc agggcatcac ctttaccgta tatagcagcg gcgaagggat
     2341 cgaaaagatc ttccccttcg atatcatccc ccgcatcatc accgccaccg aatggaacta
     2401 catcgaggcc ggcatccggc agcggctaaa agccctgaac ctctttttaa aggacgttta
     2461 caacaaccag tttatcctca aggatggcat cgtccccgtc gagatggtct acagctgccc
     2521 gcattttctc cgggaaatgc acggcttccc catcccctat gatatctatg tccatatcgc
     2581 cgggatcgac ctcatccggg accaggacgg gacattttat atcctcgaag acaacctccg
     2641 cacccccagc ggggtaagct atatgctcga gaaccgggag atcaccaaac gcatcttccc
     2701 cgacctgatc ccaaagaata atgtccgcac ggtaacagag taccccaata tcctccatcg
     2761 caacctggtg tccctttccc cccggcaaac ggcgaatccc accatcgtcc tgctgacacc
     2821 ggggatctat aactccgcct attttgaaca taccaccctc gcccggctga tgggagtcga
     2881 actggtggaa ggccgggacc tggtcgtgga taaccaccgg gtgtatatga aaaccaccgc
     2941 cggattgcaa caggtggatg tcatctatcg ccgggtggac gacggctttc tcgatccgct
     3001 cgtcttccag cccgatagca tgctgggcgt gtcgggcatc atgagcgcct accggaaagg
     3061 gaatgtcgcc atcgacaacg ccgtcggcaa cggggtagcg gatgacaagg ccatctatgc
     3121 ctttgtcccc gccatgatcc gctattatct caacgaggaa ccgatcctga agaatgtacc
     3181 cacctatcaa ttggggaacc ccgaagaacg ggaatatgta ttcgccaaca tgaaccgcat
     3241 ggtcgtaaaa aagacgaacg agagcggggg ctatggaatg ctgatgggac attcggcaga
     3301 ggaggaagag atcatgcggt ataaaacgga gatcctgaaa gacccccgcc agtatatcgc
     3361 ccagccgacc atcaatcttt cctccgcgcc ctgctacatg aatggccggc taaaaccccg
     3421 ccgcgtagac ctgcgcccct atgccctctg tggccccgat ggtatccaga tcgtccccgg
     3481 cggactgacc cgcgtggcgc tgcgcgaagg ctcgctcgtg gtgaactcct cccagggtgg
     3541 cggcagcaaa gacacctggg tgctggcggg ataatttttt ttcaaccctg taaaaccttt
     3601 caaagataca tgctgagtag agtagccgac agtttatatt ggatgagccg gtacatggaa
     3661 cgaacagatg gcatcctccg gatgctaaag atcaattacg catcctccca ggacgatatc
     3721 caggaatttt cctggaaacc cgtcctgcgc atcttctcct acctggaaga agagcaggcc
     3781 acttccattg cccatcagac ccgcaccgtg ttacagtata tggtcaccag caaggagaat
     3841 accaactccg tgctcaatat cgtcacccgg gcccgggaga acgcccgcag cgtccaggac
     3901 catatcacaa aagaactctg gcaatgcctg aacgattttt atcacaccat ccgcgatgaa
     3961 cagctggtcc gcggcctgca gaaagacgac cccgtctcca tcctcgatat cctcatccgc
     4021 cagggactcc tgtactatgg gatcaccgac attaccatgg cgcgcggcga aggctatgcc
     4081 tttatcaata tcggcaaatt cctcgagcgc ggcgtacagt cggcggatat cctcgatatc
     4141 aaattcagcg atctgcaata tgacctcaca cacaccgaca ccacctattg gaaatacctc
     4201 ctcctttcca tctcggggta tgagctctat ctaaagacct accgcagcgg cttcgacgcc
     4261 cgcaatgtag tggaacagat cgtgctgaac gaggatttcc cccggagcgt gatctactcc
     4321 atcaaccagc tccagcgcta tttcgaaagg ttgaagagcg atcgcaacaa ggacgcatac
     4381 cacaagatcg attttatgat cggccgcatc cgcagcaggg tgaagtattc caccgtcgaa
     4441 accatcctcc ggcagggact ccattcgtac ctcaacgggg taaaagaaga gctttatgag
     4501 gtagggaacg cccttaacca acactatttc gcgtacacct aaaacagacc ggcttcaata
     4561 aaacacctaa tttcgcgcct ccactatgcc ccgtttcaac atccaccata ttacgaaata
     4621 tacgtatgaa ggcccggtaa gggacagcgc caaccagatc gtgctgtttc cgataaagga
     4681 cgaattccag gagatcctca ggcatgacct gaccgttacc ggcgaacccg tggtagaggt
     4741 ctacaaagac tactatggta acgaagtggg ttccttcgcc cttgccgaac cccatagctc
     4801 cctgatcatc gactcccggg tcgaagtgat caccaagcag cgcccacccc tccgggacca
     4861 ggcggaaaag gaagcccaat gggccctgct cgaacagatc cgctggcagg tgccctatat
     4921 cgatttcctg aaacaggaac agctggcctc tctttccgaa gtgctggaaa gcatatgccc
     4981 gggcaagggc cgcaaccaga ccccgctcga agcggcacag caactacgaa cctacgtcta
     5041 cgaccagttc aaatatatca agggagtcac cagcgtcgag tcgaccctcg acgaggtctg
     5101 ggaactaaag gcgggcgtat gccaggactt tgcccatatg ctgctggtct tcctgcgcca
     5161 gatccagatc cccgcccgct atgtcagcgg gtatatctgc cccaaccaga atggaatgcg
     5221 cggggaagga gccacccatg cctggatcga agcctatatc cccttcaacg gctgggtcgg
     5281 gctcgatccg acaaacaatt gcgtgacgga cgatcttcac gtccggctcg ccgtgggccg
     5341 cagcttctcc gactgctcac ccgtcagggg aacctacaaa ggaacagccg gccagatgct
     5401 ggaggtcggc gtctccgtct cctacgagaa cggccggaca tcggaagatg ccgtaacggt
     5461 cctggctcca cagccaaccg tcaatggaaa agatattaat tcttatcgcc ggtatatgga
     5521 gatgatgcag cagcagcaac aacaacagta gtctcctcct tctcttccgt cggcgggcga
     5581 taggcctgga agggcattgc aagcagggcc cccatccggt tatgctccgc ctccccggga
     5641 tgcgtgtcga taccatagac aagccccttc gtatcacgga caaaacgata ggttccaaaa
     5701 agacggtccc agatcgaaaa gatattgcca taattggtat cggtcagggg tcgccggtag
     5761 tgatggtgaa ccttgtgcat atccggggat acgatcaccc aggccaggac attgtccagc
     5821 catttgggta aggcaaggtt ggcatggttg aactgcgaga acagcgcgga cagcgactgg
     5881 tagaggaata ccagccagac cggggtcccc gccacgatca cggcgatcag cgtaaaaaag
     5941 gcgcggaaaa aactttcccc gggatggtgc cggttggcgg tggtagtatc cacccagttg
     6001 tcgctgtgat gcaccaggtg gaatttccaa agccatttca cctggtggtg cagccagtgg
     6061 ataagccagg cgccgatgaa gtcgaggatc agcaacccca ggagcgtgaa cagccaaagc
     6121 ggtaacggca gcaggtagag caaaccggta tgatgagccg tgctgtagtc tgccgcccgg
     6181 atgatcagcg acgcaaaggc gaaattgacg atcaccgtgg tgagggtaaa aaagagattc
     6241 aaccccgcat gacgcacccg gtgatagcgg aaccgtacca tcggcacgat gccttcgatg
     6301 atccaaaaga atagaagacc tcccgcaagg atgaggctgc gttgatagga gggaatatgc
     6361 tggaaatagt agacgaggga ttccatatga ctgccgggct tccctccaat gtacgaaaaa
     6421 tttagctgtt aagcatcagc ggcatcacca gcatcaacac ctcttcgttc tcgtcctctt
     6481 cggtgggctt gatgatcccg gccttcgtcg gcgtggagag ctccatccgc acttcgtcgc
     6541 tgtcggtgga ggcaagcatc tcgatcagga agcgcgcatt gaaggcaatg gccaggtcct
     6601 ctccgtcgta ctggcatttc atccgctccg ttccttcaaa ggagaagtcg acatcctgcg
     6661 ccgccagctg cagttcactg ccctggatgt tcaggaccac ctggttggtg ctcttgttac
     6721 taaagatgct cacccggcgg agcgcactct gaaagtcgtt gcggcccacc actaatttgt
     6781 agggattgtc ggcggggatc accaccttat aatcggggaa ccgcgcgtcg atgagccggc
     6841 agctcatctg cgtcgtacca tggctgacaa agagatggtt gctgttatag ctgatggtga
     6901 tctcatcctc gttggaaggc agcgccgact tcaacaggtt gagcggcttc ttcgggacga
     6961 tgagtgaatc gctcttggga caggaaacat ccttccgctt gtagcgtacc aggcgatggg
     7021 catccgtggc gacgaactgc aatccctttt tgtccatttc gaagaagacc ccggtcatgg
     7081 ccggacgcag atcgtcattg ctgacggcga acagggtctt gttgatcgcc gttacaaggg
     7141 cggaagaagt cgtcgtaaaa gaagtggttt cgtcactggc cggctccttg gggaagttgt
     7201 cgggattctc acccatcacc ttatatttcc cattgtcgct ggtgagctcg acggcgaaat
     7261 gtttgtcgat attgaaagta agcggctgat cgggaatgtt tttcagcgaa tcgataagga
     7321 tcttggccgg gatacagacc tttcccgtat ccttcgcctc cacctccaaa tgcactttca
     7381 tgacggtttc caggtcggta gcaacaacgg ttaacttgtt cttttccacc tcgaagagga
     7441 agtcttccag gatgggtaaa acggtattcg cattgatcac accactaatc tgttgcagtt
     7501 gtttcagtaa tgccgacgag gaaacgataa acttcatgga atatatcttt gtggcgaaac
     7561 tactgatttt tgcgtaatag ccaagagatt aggaaaggcg gcaatgccga tgggatcaat
     7621 tgttaatttc ttgtgactaa actcctaatt tcacggctac caattgatta tgatgagaag
     7681 aacgacattg acccccggac aggccctcca aaaggcccgt cattactgcg gctaccagga
     7741 aagatgtcac caggaagtgc aggaaaaact ctattccttc gggctgcgga agccggaagt
     7801 ggatcagatc ctggcaaccc tgatcgaaga gaactatctc aatgaagagc ggtttgccct
     7861 ccagtacgcc ggcggtcatt tccggctcaa acgatgggga cgggtccgca tccgttacca
     7921 gctgaaacag aaacaggtat ccgaatattg tataaaaaag gccctggctt ccatcgacca
     7981 ggaagaatac gaacagacac tgctgcggct ggccgaagaa aagacgcaat ccctcgcggt
     8041 ggaaacaaat cccttcgccc ggaaacagaa actgcaggaa tacctgctca ggaaaggata
     8101 tgaacccgac cgggtagccg ccgtggtagg ctccgccgcc caatgaccgc agccgccaaa
     8161 taatgagtac attgcagtat gtcctcattg accattacga ccatccagcc cgacctggtc
     8221 tgggaagaca agaccgccaa ccttcaacgg ctggaaaaac agatcctttc catcccggac
     8281 aacaccgaga tcgtcgttct gccggagatg ttcagcacgg gctttagcat gcggcccgaa
     8341 gctcttgccg aatcgatgga aggacccacc ctaaactgga tgcgcgggat cgccgcccgg
     8401 cggaagatca tcctcaccgg cagcgtcatc atcgaagaag gaggacagta tttcaaccgg
     8461 ctgatctgga tgctgccaaa cgggcaattc ggctactatg acaaacgaca ccggtttgcc
     8521 tacgcgggcg agaacgaaca ctataccgcg ggacagaaac gactggtcgc ctccgtaaaa
     8581 ggctggcggg tgctgctgct cgtgtgctac gatctccgtt ttcccgtctg gagccggcag
     8641 accccgcagg accccgaagc ccctgcggat tcacccctcg aatacgatct cctcctcttt
     8701 gtcgccaact ggcccgaacg ccgctctctg gcctggaaaa cgcttctcca ggcccgcgcg
     8761 atcgagaacc aggccttcgt cgtgggtgtc aaccgcgtcg gcaacgacgg gaacaacatc
     8821 tatcatagcg gggactccct gatcatcgat cccctcgggg agatccttta tcatggattc
     8881 ggcaaagaag aggtggttac ccatacgctc caaaaggaaa gattggaaga ggtgcggcaa
     8941 cgcttcccct tttggcggga tgcggatcat ttctccatag aaccataaaa agaacccaaa
     9001 caagtacgct aaaacagacc gatgtcagca acagcattca tcgcccggga gatcttcacc
     9061 gggagcga
//
LOCUS       DATGFG010000044         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_566307, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000044 DATGFG010000000
VERSION     DATGFG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566307"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..599
                     /locus_tag="VKQ52_00875"
     CDS             120..599
                     /locus_tag="VKQ52_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLZ85752.1"
                     /translation="MAKYTLLINGKNTPIEADEKMPLLWVIREHVGLTGTKFGCGIAQ
                     CGACTVLLNGNPVRSCSIPASAVAGQPITTIEGLSPDNSHPVQKAWIAEQVPQCGYCQ
                     SGQILAAVALLNKTPHPTDENIDMAMQGNICRCGCYTRIRKAIKTASASLKPAATLK"
     gene            606..>2506
                     /locus_tag="VKQ52_00880"
     CDS             606..>2506
                     /locus_tag="VKQ52_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLZ85753.1"
                     /translation="MQPKYPARSRRQFITDAGLSGLALVLGAYLPGCTSNTQAPKVFN
                     ASGGALPQATELMTWISIDNTGKVSILNHRAEMGQGAWQVVPQMIAEELEVSLDQVNI
                     LFAPGNQKKYGSQVTGGSSTVRGSYKHLLHIGASAREMLTEAAAKKWDAKTSDCYAEN
                     GQIFHRPSGRSFTYGQLVEDAAKLQPPKDVQLKSRKDYKLVGKPLPRKDIPLKTNGAA
                     IFGIDKKLPGMLYAVVERSPRIRGKVKSIDDSAAKSIPGVRHVITVQRTVFDHLQDGV
                     AVVADNLWAAMEGRKQLKVEWDDTGFEHLSTEQLFARMHEDLKKPGLSDRTGGDFESA
                     WQKAPTKLEAVYEAPYQAHACMEPLNCIADVKEHSIDIWGPIQGPDWVQSDLSERMGI
                     PLDKVTVNMTFLGGGFGRKAFLDYPHEAAVISKAVKAPVQVVWTREDDITQGPFRPGA
                     VYACKAGLSKEGHVEAFQTKSATQNMNHENPGVDLHSYNGDTLDGLCKPYYDSFSNYR
                     FADIPTVSPIPVMWWRSVYASTNGFAYESFMDEMAHAAGKDPLEFRRQHLDNDRYQAL
                     IDQLITITSWNSRGKNQGWGVAITECFNSIVGEVVKVSRKPDGKIKIDKVTALMDCGW
                     YVNPDIIRA"
BASE COUNT          637 a          883 c          580 g          406 t
ORIGIN      
        1 tacatctggg acgaaaatat ccccctgccg caataacagg cgcaccaacc actcttataa
       61 ttttttatcg tggcttcaaa aaattcggta acttttctca tcccaaatac actaccgcca
      121 tggcaaagta taccctcctc ataaacggga agaacacacc catcgaagcc gacgaaaaaa
      181 tgcccctgct ctgggtgatc cgcgaacacg tcggcctcac agggacaaag ttcggctgcg
      241 gcatcgccca atgcggcgca tgcaccgttc tcctcaacgg caatcccgtc cgctcctgct
      301 cgattcccgc atccgccgtc gccggccagc ccatcaccac gatcgaaggt ctctccccgg
      361 acaattccca cccggtgcaa aaagcctgga tcgccgaaca ggtcccgcaa tgcggctact
      421 gccagagcgg ccagatcctc gccgccgtcg ccctcctgaa caaaacccca catcccaccg
      481 acgaaaatat cgacatggcc atgcagggta acatctgccg ctgcggctgc tacacacgca
      541 tccgcaaagc cataaaaaca gcctcagcct ccctcaaacc agccgccaca ctcaaataaa
      601 caattatgca acccaaatat cctgctcgat cccgccgcca attcataacg gacgccggcc
      661 tctccggcct ggcactcgtc ctcggcgcct atctgcccgg ctgcacaagt aacacccagg
      721 cgccaaaagt tttcaacgcc tccggcggcg ccctccccca ggccaccgaa ctgatgacct
      781 ggatctccat cgacaacacc ggtaaagtgt cgatactcaa ccatcgcgcg gaaatgggcc
      841 agggcgcctg gcaggtcgtc ccccagatga tcgccgaaga gctcgaagtg agcctcgacc
      901 aggtgaacat cctcttcgcg cccggcaacc aaaagaaata cggcagccag gtcaccggcg
      961 gcagctccac cgtccgcggt tcatacaaac atctcctcca tatcggcgcc tccgcacgcg
     1021 aaatgctcac cgaagccgcc gcaaaaaaat gggatgccaa aacaagcgac tgctatgcgg
     1081 agaacggcca gatcttccac agacccagcg gcagatcctt cacgtatggc caacttgtcg
     1141 aagatgccgc gaaactccag ccgccaaaag acgtacaact aaagtccaga aaagactata
     1201 agcttgtcgg caaaccgctc ccccgcaagg atatcccgct aaaaacaaac ggagcggcca
     1261 tcttcggcat cgacaaaaaa ctcccgggca tgctctatgc cgtcgtcgaa cgcagccccc
     1321 gcatccgcgg caaggtcaaa agcattgacg atagcgcagc aaaatccatc cccggcgtcc
     1381 gccacgtcat caccgtacaa cgcaccgtct tcgatcacct ccaggatggc gtcgccgtcg
     1441 tcgccgacaa cctctgggca gccatggaag gccgcaaaca actaaaagtg gaatgggatg
     1501 acaccggctt cgaacacctc agcaccgaac agctcttcgc gcgcatgcac gaagacctga
     1561 aaaagcccgg cctctccgac cgaaccggcg gcgatttcga gtccgcctgg caaaaagcgc
     1621 cgacaaaact ggaagccgtc tacgaagccc cctaccaggc ccacgcctgc atggaacccc
     1681 tcaactgcat cgccgacgta aaagaacatt ccatcgatat ctggggtccg atccagggcc
     1741 ccgactgggt ccagtccgat ctcagtgagc gcatgggcat tccgctcgat aaggtcaccg
     1801 tcaacatgac ctttcttggc ggcggcttcg gccggaaagc cttcctcgat tacccccacg
     1861 aagcagccgt catctcaaaa gccgtcaagg cccctgtcca ggtcgtctgg acccgcgaag
     1921 acgatatcac ccagggcccc ttccgccccg gcgccgtcta tgcctgcaag gccggtctta
     1981 gcaaggaagg ccatgtagaa gccttccaga cgaagagcgc cacccagaac atgaaccacg
     2041 aaaatcccgg cgtcgacctc cattcctata acggtgatac cctcgacggc ctctgcaaac
     2101 cctactacga ctccttctcc aattataggt tcgccgatat ccccaccgtc tcccccatcc
     2161 ccgtgatgtg gtggcgatcg gtctatgcct cgacaaacgg cttcgcctac gagagcttca
     2221 tggacgaaat ggcgcacgcc gcgggaaaag acccgctgga attccgccgg caacacctcg
     2281 acaacgaccg ctaccaggcc cttatcgacc agctgatcac catcaccagc tggaactccc
     2341 ggggaaagaa ccagggctgg ggcgtcgcca tcaccgaatg ctttaacagc atcgtcggcg
     2401 aagtggtaaa agtctcccgg aaacccgatg gaaaaataaa gatcgacaag gtcaccgccc
     2461 tcatggactg cggctggtac gtaaaccccg acatcatccg cgcaca
//
LOCUS       DATGFG010000045         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_566040, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000045 DATGFG010000000
VERSION     DATGFG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566040"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VKQ52_00885"
     CDS             complement(<1..664)
                     /locus_tag="VKQ52_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85754.1"
                     /translation="MKRILSILLFSGGWLFTQAQTIVLSAGSTKTLTIKSGTVFSADS
                     LVLTPSADVTLSGNTLTESPTPLAIAPNPSIKRVYTFSAQLVYSGTVQVYYNPATELN
                     GNTEANLEYTDSAVGGAWLPSATSTVNTSLHYVQQSLSAHSFIGVTAAGKATTLPLSL
                     ISFTGSWQGNSVPLRWVVEQTSEPVNFTVYSSTDASAWQVAGMVSGVPAAGTYTYDYT
                     DFG"
     gene            complement(686..1972)
                     /locus_tag="VKQ52_00890"
     CDS             complement(686..1972)
                     /locus_tag="VKQ52_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85755.1"
                     /translation="MRQIYLAAFMLLLSAALHAQVGIGTASPLSTLDVRGSISPGYRS
                     FSSSTTLTASDYTALFTGSSAATVTLPDATTCPGRIYCIKNFSATSPAPLVTIATASS
                     QKIDGAANWLLNETNETVTLSSDGANWEVFSSVAPSSAGGSWNEGGNGVAAVQNLGTT
                     TNYDLPFITNNTERMRITNGGYVGMGSTSFSANPEALLVYQNNSTSFNVIAGKGNLNN
                     YLQLNIQNKNSGTSASSDLVATADNGNETTNYVDLGMNSSGYSASGITGGADNAYLYS
                     AANDFVIGNSTSGKNLIFFTGGTATTNERMRIASGGNVGINNASPAATLDVAGTVKIG
                     TAGTALNSVIRFTNQSVTDNVAFNYNQTRTESLTLTGVSQYATVIVNPRSSLPTGLGI
                     AFSYASATNTVQVVLINASGSTLALGTVVFDVTVIQ"
     gene            complement(2001..>3347)
                     /locus_tag="VKQ52_00895"
     CDS             complement(2001..>3347)
                     /locus_tag="VKQ52_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ85756.1"
                     /translation="LRDCIEEVLQLFIGQAARTGVELLYDIDPEVPEQIRGDRKRLNQ
                     VLVNLVENAVRHTETGEITISAGMLSQPEKDELELCFEVRDTGTGIAADKMEQLFKGI
                     GVPEGADDVAGKPAGLGLIICKRLVESMRGFIQASSEAGEGSQFTFSIRVARSQQQAR
                     KTAAQQRTVFEGKRVLVVDGRAGRRAILVRRLEKLGLIPVAADSVRHALEILSRRPGT
                     DAVVIDGEAGEIGPLVDAARKLGPGIPVLAIGGSAAADGLAGRPADGSNGSGPANGLA
                     FDAVLARPVRQHLLSDQLLQLLTLPAGERVHSDAGKLLEDRFSGDHPLRILVAEDHPV
                     NQKLAMTVLTKLGYTPDLAGNGKEALDMVSHQNYDIILMDVQMPEMDGLEATRMIRLC
                     VQPQPVIIAMTANAMQGDRDRCLQAGMDDYISKPLELDELVAQLKKWSAVIREKIK"
BASE COUNT          699 a         1057 c          904 g          687 t
ORIGIN      
        1 gcccgaaatc ggtatagtca tacgtatagg ttccggctgc gggaactccc gaaaccatcc
       61 ccgccacctg ccaggccgaa gcatccgtac tgctgtagac ggtgaaattc accggctcac
      121 tcgtctgctc gaccacccat cgcagcggta cactgttgcc ctgccaggac cccgtaaagg
      181 agatcagcga aagcggcagc gttgtggcct tacccgccgc cgtcacgccg ataaaggaat
      241 gcgcggaaag actctgctgt acatagtgca gcgaggtgtt gacggtactg gtggccgatg
      301 gcagccaggc gccgcccacg gcggaatccg tatactccag gttggcctcg gtgttcccgt
      361 tcagttcggt cgccgggtta taatacacct gcaccgttcc cgaataaaca agctgcgccg
      421 aaaaagtata cacccgcttg atactcggat tgggcgcgat cgccaagggg gtaggggact
      481 ccgtcagggt gttgccggaa agcgttacat cggcggatgg cgtcagcacc aggctgtcgg
      541 cgctaaacac cgtcccggac ttgattgtca gcgtcttcgt gctgccggcc gaaaggacga
      601 tagtttgggc ctgcgtgaac agccagccgc cggaaaaaag cagtatagat aaaattcgtt
      661 tcatttcatt cggatttatt gtaccctatt ggatcaccgt aacatcaaaa accaccgttc
      721 caagcgccag cgtagaaccc gatgcattga tgagcaccac ctgcacggta ttcgtcgcgg
      781 acgcatagga aaaggcgatc cccaaacccg tcggcagcga actgcgggga ttgacgatca
      841 ccgtggcata ctggctgaca cccgtcaggg tcaggctctc cgttctcgtc tggttatagt
      901 tgaaggcgac attgtccgtc acactctggt tggtgaaccg gatcaccgaa ttgagcgccg
      961 tacccgccgt gccgatcttg accgtgcccg ccacatcgag ggtggccgcg gggctggcgt
     1021 tattgatacc cacattcccg cccgaagcga tccgcatccg ctcatttgtc gttgccgttc
     1081 cccccgtaaa gaagatgaga tttttgccgg acgtgctgtt gccgatgaca aaatcattgg
     1141 cggcgctgta gagatacgca ttgtcggcgc ccccggtgat cccgctggcg ctatagccgc
     1201 tcgaattcat cccgaggtcc acatagttgg tcgtctcatt cccgttgtcg gccgtggcca
     1261 caagatccga agaagcactg gtgccgctgt tcttgttctg gatattcaat tgcaggtaat
     1321 tgttcaggtt acccttgccg gcaatgacat tgaacgaagt ggaattattc tggtagacaa
     1381 gcagggcctc cgggttggcg gaaaaagacg ttgaacccat accgacataa ccgccattcg
     1441 tgatccgcat ccgctcggta ttattggtga taaagggcag gtcgtagtta gtggtagtgc
     1501 ccaggttctg caccgccgcc accccgttac ccccctcatt ccaggagccg ccggcggaag
     1561 agggcgccac cgagctgaag acctcccagt tggcgccgtc gctggacagc gttaccgtct
     1621 cattggtctc attcaataac cagttggcgg cgccatcgat cttctgcgat gaagccgtgg
     1681 cgatcgtcac cagcggcgcc ggtgaagtgg cgctgaaatt cttgatacaa tagatcctcc
     1741 ccggacaggt ggttgcatcc ggtaaggtaa cggtggccgc cgaactaccg gtaaataggg
     1801 ccgtatagtc ggaagccgta agggtggtgg aactactgaa cgaccggtaa ccgggcgata
     1861 tagagccccg gacatcgagt gtcgaaagcg gcgaagctgt gccgatgccc acctgggcat
     1921 gaagcgcggc ggaaagcagc aacataaaag cggcaaggta gatctgtctc ataaaagggt
     1981 atttcaattt ttgggtttta ctattttatt ttttcccgga tgaccgcgct ccatttcttc
     2041 agctgcgcga ccaattcatc cagttccagt ggtttgctga tataatcatc catccccgcc
     2101 tggagacaac gatcccggtc gccctgcatg gcattggccg tcatggcgat gatcaccggc
     2161 tgcggctgca cacaaaggcg gatcatccgc gtcgcctcga gcccatccat ctccggcatc
     2221 tgcacatcca tcaggatgat atcatagttc tgatggctca ccatatccag ggcttccttt
     2281 ccattacccg ccagatccgg ggtataaccc agtttggtca gcacggtcat ggctaatttc
     2341 tggttcaccg ggtgatcctc cgccaccagg atacgcaacg gatggtcccc ggagaaccgg
     2401 tcctccagca acttgcccgc atccgaatgg acacgctcgc cggcaggtag cgtcagcaac
     2461 tgcagcagct ggtcgctcag cagatgttgc ctcaccggcc gggccagtac ggcatcgaaa
     2521 gccaggccat tcgccggccc actgccattg gaaccatcgg ccgggcgccc ggccaaacca
     2581 tcggcagcag cagatccgcc gatcgccagc acggggatgc ccgggcccag cttcctggcc
     2641 gcatccacca acggcccgat ctcaccggct tccccatcga tgacaacggc atccgtcccc
     2701 ggacgccggg aaaggatctc cagcgcatgt cgcaccgaat ccgcggccac ggggataagc
     2761 cccagctttt ccaaacgccg cactaaaatt gcccggcgcc cggccctgcc gtccactacc
     2821 aacacacgct tcccttcgaa aacggtccgc tgctgggcgg ctgttttacg cgcctgctgc
     2881 tgactccgcg caacgcggat gctgaaggtg aactggctgc cctcgccggc ctcgctcgac
     2941 gcctggataa aaccccgcat gctttccacc agccgtttac agatgatcag gccgagacca
     3001 gccggcttgc ccgccacatc atcggcccct tccggaaccc cgatcccttt gaacagttgt
     3061 tccatcttat ccgcggcgat gcccgtgccc gtgtcccgga cctcgaaaca caattccagt
     3121 tcatccttct ccggttggct gagcatcccc gcactaatag tgatctctcc cgtttccgta
     3181 tgccggacgg cattctccac gagattcacc agcacctggt tcaaccgctt gcggtcaccc
     3241 ctgatctgct ccggaacctc ggggtcgata tcatacaaca actcgacacc cgttcgcgcc
     3301 gcctgcccga tgaaaagctg caacacctcc tcgatacaat cgcgcag
//
LOCUS       DATGFG010000046         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_449997, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000046 DATGFG010000000
VERSION     DATGFG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449997"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VKQ52_00900"
     CDS             <1..392
                     /locus_tag="VKQ52_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HLZ85757.1"
                     /translation="VPGYGHAVLRKTDPRFSAQMEFGKKHMPDDPLVRTVWNIYETVP
                     PILQSLGKIKNPWPNVDAHSGALLVHYGLVEYEFYTVLFGVSRALGVLASLCWDRSLG
                     FPLERPKSVTTELVKDWLKGEGEIWGE"
     gene            complement(601..1761)
                     /locus_tag="VKQ52_00905"
     CDS             complement(601..1761)
                     /locus_tag="VKQ52_00905"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF006003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HLZ85758.1"
                     /translation="MSTHRITKAIRIRADQTPEKEHSSPLYLTSSFCYEDAEEMRAVF
                     ADEKEGNIYSRFSNPTVQEFTDRLAALEGTETGFATASGMSAIVATFLTFLKKGDHLL
                     ACNAIFGSTHTIVTKYLPKYGIDYTYVGTDTSTWEAAIRPNTRMIFLETPTNPGLDIV
                     DLAAAGAIARKHNLIFNVDNCFATPIGQTPIDYGADLIVHSATKWLDGQGRVLGGAVL
                     GKKELIKEVYLFCRSTGPSLSPFNAWVLSRSLETLDVRMERHSSNALYLAQALESHPA
                     ISTLRYPLLPSHPQYDIARKQMKNGGGILCFELKGGLEAGRKFLDKLQMLSLTANLGD
                     TRSIASHPASTTHAKLTPEERATVGITPGLIRISVGLEHRDDILADIKNGLA"
     gene            complement(1790..2221)
                     /locus_tag="VKQ52_00910"
     CDS             complement(1790..2221)
                     /locus_tag="VKQ52_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLZ85759.1"
                     /translation="MIRIELDRVSGNFGFEAKDAAGHTVRLDSSPETGGADFGVRPMQ
                     MLLMGLGGCSGIDIVSILNKQRQTVEAFHMTIDGEREKGKEPTLWETVHIHYALKGAI
                     DPDKAKKACELSMDKYCSVAATLREARCKITWQVSVNGQTI"
     gene            2620..>2999
                     /locus_tag="VKQ52_00915"
     CDS             2620..>2999
                     /locus_tag="VKQ52_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85760.1"
                     /translation="MNYFTSEHPFALEAGGSLPGITVGYHTYGRLNADRSNVVWVCHA
                     LTANSDVARWWPGVVGEHGAIRPEDYFIVCANILGSCYGSSGPLGAGPGIGEHGVGRQ
                     EGAGSGQGEVPGAGGSAVVDLARVG"
BASE COUNT          517 a          620 c         1133 g          729 t
ORIGIN      
        1 tggttcccgg ttatggtcat gcggtgttgc ggaagaccga tccgcgtttc tccgcgcaga
       61 tggagtttgg caagaagcat atgccggacg atccgcttgt acggacggtg tggaatatct
      121 atgagacggt gccgccgata ctgcagtcgc ttggaaagat aaagaatccc tggcccaatg
      181 tggatgcgca tagcggtgcg ttgctggtgc attatgggtt ggtggagtat gagttctata
      241 ccgtattgtt cggggtgagc cgggcgcttg gggtgctggc gagcctttgc tgggaccggt
      301 cgttgggttt cccgctggag cggccgaagt cggttacgac ggagctggtg aaggattggt
      361 tgaagggaga aggcgagatc tggggggagt agccgaggac cgaggttgcc ggagggacgc
      421 ggctgtcgtg ggaggaccga ggttgcggtg cgaatgccgg atcagtggtc ggaaggacgc
      481 ggctatcgcc ggaggaccga ggttgtgaca ggtgaacgag gccctggcgg gagtgccggg
      541 gcggtggccg gaggacggtg ttgccgcgcg gcgtgatcga aaaaaagata ttgggttggc
      601 ttaggccaac ccatttttta tgtctgcaag gatgtcgtcg cggtgttcga ggcctacgga
      661 gatgcggatg aggccggggg tgatgccgac cgttgcgcgt tcttcggggg tgagttttgc
      721 gtgggtggtg cttgcggggt gggaggcgat gctgcgggtg tcaccgagat tggcggtgag
      781 ggagagcatc tgcagtttgt cgaggaattt cctgccggct tcgaggccgc cttttagttc
      841 aaagcagagg atgccgccgc catttttcat ttgtttccgg gcgatatcgt attgcgggtg
      901 gctggggagc agggggtagc ggagggtgga gatggcggga tggctctcga gggcctgggc
      961 gaggtagagg gcgttggagg aatgtctttc catccggacg tcgagggttt cgaggcttcg
     1021 gctgaggacc caggcgttga agggggagag ggagggcccg gtgctgcggc agaagagata
     1081 gacctccttt atcagttctt tttttccgag cacggcgccg ccgaggacgc ggccctggcc
     1141 gtcgagccat tttgttgccg agtggacgat gaggtcggcg ccgtagtcga tgggggtctg
     1201 gccgatgggg gtggcgaagc agttatcgac gttgaatata aggttgtgtt tgcgggcgat
     1261 ggcgccggcg gcggcgaggt cgacgatgtc gaggccgggg ttggttggtg tttcgaggaa
     1321 gatcatccgg gtattgggcc ggatggcggc ttcccaggtg gaggtatcgg tgccgacata
     1381 ggtgtagtcg atgccgtatt tgggaaggta ttttgtgacg atggtatggg tgctgccgaa
     1441 gatggcgttg caggcgagca ggtggtcgcc ttttttcagg aaggtgagga aggtggcgac
     1501 gatggcgctc atgccggagg cggtggcgaa gccggtctcg gtgccttcga gggctgcgag
     1561 gcgatcggta aattcctgga cggtggggtt gctgaagcgg ctatagatat tgccttcttt
     1621 ttcgtcggcg aagacggccc gcatttcttc ggcgtcttcg tagcagaagc tgctggtgag
     1681 gtagaggggg gaggagtgtt ctttttccgg ggtctggtcg gcgcggatgc ggatggcttt
     1741 tgtgatgcgg tgggtggaca tgctattttg cagttgggtg aagaataggt tatatggttt
     1801 ggccgttgac gctgacctgc caggtgatct tgcagcgggc ttcgcggagg gtggcggcca
     1861 cggagcagta tttgtccatg gacagttcgc aggctttttt tgccttatcg gggtcgatgg
     1921 cgcctttcag ggcgtaatgg atgtggacgg tttcccagag ggtgggttct tttccttttt
     1981 cgcgttcgcc gtcgatcgtc atatgaaagg cttctacggt ctgtctttgt ttgtttagga
     2041 tggacacgat atcgatgccg ctgcaaccgc cgaggcccat cagcagcatt tgcatggggc
     2101 gtaccccgaa gtcggcgccg cccgtttcgg ggctgctatc caggcgtacg gtatgtccgg
     2161 cggcgtcttt tgcctcgaaa ccaaagtttc cgctaaccct atccaattcg atcctgatca
     2221 taaatggaag atttttgcaa atttacgcat aaaccgccgg aggcggtttg tccgaagaca
     2281 gaaacaattg accgaagcga aagttgtgga cgagggcttg ccggtggcag gccaaagtcc
     2341 ttaatttatc ggaaatgccc tatttttttt gggaagggca tttccggatg aaatgcaagc
     2401 ccttattttg catcgaaatt tgggtggggg ccggaaccgg gcgagtccaa gaccgggccg
     2461 cgagtccaaa acgggtgggt tgcgagtcca gaccgggtga gcccggttag tacatgaggc
     2521 cgtaggccga aatgtaccga cattatgcgg gtcgcggcaa aaaattattt tgtggcaatt
     2581 ttttgccgcg agtcctattt gttcacgaga attggcttca tgaattattt tacatctgag
     2641 cacccttttg cgttagaggc gggtgggtct ttaccgggta ttacggtggg ttatcatact
     2701 tacgggcggc tgaatgcgga ccggagcaat gtggtatggg tatgtcatgc gttgacggcc
     2761 aattccgatg ttgcacgatg gtggccgggg gttgtcgggg agcatggggc gatccggccg
     2821 gaggattatt ttatcgtttg cgccaatatt cttggttcct gttatgggag ttcgggtccg
     2881 cttggggcgg gtccggggat tggggagcat ggagtggggc ggcaggaggg agccggcagt
     2941 gggcaagggg aggtgccggg ggccgggggg agtgcggttg tagatttggc gcgggtggg
//
LOCUS       DATGFG010000047         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1362500, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000047 DATGFG010000000
VERSION     DATGFG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362500"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VKQ52_00920"
     CDS             complement(<1..233)
                     /locus_tag="VKQ52_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85761.1"
                     /translation="MVSFPNCKINLGLSILRKRTDGYHDLSTVFYPLPVKDILEIIHT
                     PTPIHTPTPSPTPAFTAYGIPIPGDSTSNLCEKA"
     gene            complement(269..874)
                     /locus_tag="VKQ52_00925"
     CDS             complement(269..874)
                     /locus_tag="VKQ52_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HLZ85762.1"
                     /translation="MKKIELEIVALSHSITQTHSYAVVLGEVNGLRRLPIVIGGFEAQ
                     AIAVALEKMHPSRPLTHDLMKNFMSAFTIDLQEIIICDLQEGIFYSKLVCVSEHDTVE
                     IDSRTSDALALAVRFGCPIFTYEHILDSAGILMEDSSSKKKKTSPTSTVSTTDSGGTR
                     EDLKVLSMDDLTSLLNEVLEQEDYIRAIAIRDEINSRRKTR"
     gene            complement(959..1921)
                     /locus_tag="VKQ52_00930"
     CDS             complement(959..1921)
                     /locus_tag="VKQ52_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HLZ85763.1"
                     /translation="MSVFIFIDHSDGHVKKASLEALSYGAALATQLNTTAEGILLGTL
                     SSDPALLGKYGIKKIHQAAQESLNALDAQVYAKIIADLVGAAGESVLVFSNNLNGKAV
                     APRVAARLKAALVTGAIALPNTSNGFVVRKTVFSGKAFANISLNTPIKVIALNPNAYA
                     VKEGEGKAEITALAVSAPAPKVKVVSTTAATGHVPLTEAELVVSGGRGLKGPENWGMI
                     EELASLLGAATACSRPVADAHWRPHNEHVGQTGIAIAPNLYIAIGISGAIQHLAGVNR
                     SKVIVVINKDPEAPFFKAADYGIVGDAFEVVPKMIAAVKKLKGV"
     gene            complement(1953..2696)
                     /locus_tag="VKQ52_00935"
     CDS             complement(1953..2696)
                     /locus_tag="VKQ52_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HLZ85764.1"
                     /translation="MKILVCISKTPDTTAKIAFTNNNTTFSGDGVQWIINPYDEWYSL
                     VRAIELKEKDPSTAIHLITVGLADTEPIIRKALAIGGDEAIRVNLESQDPYTIAVQIA
                     EAAKSGGYDLIFTGKETIDYNGSAIGGMVAELLDLPYISLATKFELGGTTATVTREIE
                     GGEEVDEVALPLVVSCQKGVAEQRIPNMKGIMGARTKPLKVVEPVATQALTSIVSFEL
                     PPAKAGVKLVSADQPEELIRLLHEEARVF"
     gene            2800..3135
                     /locus_tag="VKQ52_00940"
     CDS             2800..3135
                     /locus_tag="VKQ52_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ85765.1"
                     /translation="MDRIEKLKEFLLADPNDSFVRHALALEYGKTGDVAMARKLFEEV
                     LERDPAAVGTYYHLGKLLEGIGETGLALSWYEKGMVAAKAAMASGGKSSALRAYNELR
                     AAYDELSEE"
     gene            3149..>4444
                     /gene="tilS"
                     /locus_tag="VKQ52_00945"
     CDS             3149..>4444
                     /gene="tilS"
                     /locus_tag="VKQ52_00945"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLZ85766.1"
                     /translation="MDLLQGFKDHIVKERLFAPGERLLLAVSGGLDSVVLAELCHRCG
                     FDLIIAHCNFQLRGEESVRDEEFVQRLGVRYGWEVVTGRFDTGEYAAANKVSIQVAAR
                     ELRYRWFGEFVGSGRVRWIVTAHHLDDNIETMLMNFFKGTGIAGLRGILPKQGNIVRP
                     LLFARKEELRAFAVEAGLEWVEDSSNESDKYTRNFFRHQLIPLVEQVYPAASANLAYN
                     LGRFREVEALYRQSVEGWKKKLLEYKGNEVHIPVEKLRKAAPLATIVYEIIEPYGFSP
                     QQVGEVMALMDSGSGRFVRARDFRILKNRNWLIITPLTEEQATNILVEEGRAAVSCAE
                     GVLRLARMDAAGMGALDQGPSVALLDAGKLSFPLLLRRWKAGDYFYPLGLRKKKKVAR
                     FLIDSKVSIAEKERVWVLEMDKKILWVIGRRIDDRWKVGT"
BASE COUNT          845 a          952 c         1549 g         1098 t
ORIGIN      
        1 gctttctcgc agaggttgct ggtgctgtca ccggggatgg ggatgccata ggcggtgaag
       61 gcgggagtgg gggagggagt gggagtgtgg atgggagtgg gagtgtggat gatctcgagg
      121 atatctttaa ccgggagggg atagaagacg gtggacagat cgtgataacc gtccgtgcgt
      181 ttgcgcagaa tgctcaggcc aagattgatc ttgcagttgg gaaaggatac catgctattg
      241 ggcggcgggc gttagtgtcg gtaacgggtc accgggtttt acgccggctg ttgatctcgt
      301 cgcggatggc gatggcgcgg atatagtctt cctgttccag gacctcgttt agcagcgagg
      361 tgagatcgtc catggataag actttcaggt cttcgcgtgt accaccgcta tctgtagtgg
      421 acaccgtgga agtgggactt gtttttttct ttttcgagga gctgtcttcc atcaggatac
      481 cggcgctatc gaggatatgt tcgtaggtga agatggggca gccgaagcgt acggcgagtg
      541 cgagggcgtc gctggtgcgg gaatcgatct cgacggtgtc gtgttcgctg acgcagacca
      601 gtttcgagta gaagatgcct tcctgcagat cgcagataat gatctcctgc agatcgatgg
      661 tgaaggcgga catgaaattc ttcatgaggt cgtgcgtgag ggggcggctg gggtgcattt
      721 tttccagggc gacggcaatt gcctgagcct caaacccccc gatcacgatg ggaagacgtc
      781 ttaggccgtt cacttccccc agcacaactg cataggaatg agtctgcgtg atgctatgtg
      841 acaaagccac tatttccagt tcgatcttct tcatatgaat aatatgcgaa actaacgttt
      901 ttttataaaa aaaagaccgc ctctggcgag gcggtttttt attttaattt ttaagacgtt
      961 agaccccctt caatttcttt accgccgcta tcattttggg caccacttcg aatgcatccc
     1021 ctacgatacc gtagtcggct gccttgaaga agggtgcttc ggggtctttg ttgataacaa
     1081 cgatgacctt gctccggttc actcccgcca ggtgttggat ggcgccggag atgccgatgg
     1141 cgatatacag gttgggtgca atggcgatgc cggtctggcc gacgtgttca ttatgcgggc
     1201 gccagtgggc gtctgcgaca ggtcggctgc aggctgtggc tgcgcccagg agggaggcca
     1261 gttcttcgat cattccccag ttttcagggc ctttgagccc ccgtccgccg ctgacgacca
     1321 gttcggcttc ggtgaggggg acatgacccg tagcggcggt ggtggacacc actttcactt
     1381 ttggggccgg tgcggagacg gcgagcgctg ttatttcggc cttgccttca ccttctttta
     1441 cggcgtaggc attggggttg agggcgatga ccttgatggg ggtgttcagg gagatgttgg
     1501 cgaaggcctt gccggagaat acggtctttc ttacgacgaa gccgttgctg gtgttgggca
     1561 gggcgatggc ccccgtgacg agggcggctt tcaggcgggc ggctacgcgt ggtgctacgg
     1621 ccttgccgtt taggttattg gagaacacca ggacgctttc tcctgcggcg ccgaccaagt
     1681 cggcgatgat ctttgcatat acctgggcgt cgagcgcgtt gaggctttcc tgtgcggcct
     1741 ggtggatctt ttttatgccg tacttaccca gaagggcggg atccgaagaa agggttccca
     1801 gaaggatccc ctccgcagta gtattgagtt gggtggcgag ggcggcaccg tagctcagcg
     1861 cttcgaggga agcttttttt acgtgaccgt cggaatgatc tatgaagatg aagactgaca
     1921 tggcaatttt tttttgaggt ttttctgtct cgttagaata cccgggcttc ttcgtgaagg
     1981 aggcggatga gttcttcggg ctggtcggcg gagaccagtt tcacgccggc cttggcgggc
     2041 ggcagctcga aggagacgat gctcgtgagc gcctgtgtcg cgacgggttc taccaccttc
     2101 aggggttttg tccgggcgcc catgatgcct ttcatattgg ggatgcgttg ttcggctaca
     2161 cctttctggc agctaacgac caggggcagg gctacttcgt cgacctcttc gccgccttcg
     2221 atctcgcggg tgacggtggc tgtggttccg ccaagctcga attttgtggc gagggagata
     2281 taggggaggt cgaggagttc ggccaccatg ccgccgatgg ccgagccgtt gtagtcgatg
     2341 gtctctttac ccgtgaaaat gaggtcatag ccgccgcttt ttgccgcttc ggctatctgt
     2401 acggcgatgg tataggggtc ctggctttcg aggttgacgc ggatggcttc gtcgccgccg
     2461 atggccaggg ccttgcggat aatgggttcg gtatccgcca agccgacggt gatcaggtga
     2521 atggccgtgg aaggatcttt ctctttcagt tcgatggccc tgacgaggga ataccattcg
     2581 tcataggggt tgatgatcca ttgcactccg tcgcccgaaa aggttgtatt attgttggtg
     2641 aaagcgattt tcgccgttgt gtccggcgtt ttactgatgc atactaagat cttcattatt
     2701 cgtgggtttt tgaaggaagt tgtttatgca actaaaacaa agataagcgt tgtccggtat
     2761 attcgggaat aatttatact tttttttgag ggggtattta tggacagaat cgagaaattg
     2821 aaggagttcc tgctggcgga cccaaacgac tcttttgtca ggcatgcgtt ggcattggag
     2881 tatggaaaga caggggatgt cgcgatggcg aggaagcttt tcgaggaggt gctggaacgt
     2941 gatccggcgg cggtgggaac ctattatcat ttggggaagc tgctggaggg gatcggggag
     3001 acggggctgg cgttgagctg gtatgagaag gggatggttg ccgcgaaggc ggcgatggca
     3061 tccggcggga agtcttctgc tttgcgggcc tataatgaat tgcgggcggc ctatgatgaa
     3121 ttgagtgaag aatgagtatt tttaccggat ggacctgcta cagggattta aggatcatat
     3181 tgtcaaggag agattgttcg cgccgggtga gcggttgttg ctggcggtga gcggcgggct
     3241 ggattccgtt gtgctggcgg aactctgtca ccggtgtggg ttcgatctta tcatcgcgca
     3301 ttgcaatttt cagctgcggg gggaggagag tgtgcgggat gaggaatttg tgcagcggtt
     3361 gggggtgcgg tatggatggg aggtagtgac ggggaggttc gataccgggg agtatgcggc
     3421 ggcgaacaag gtttcgatcc aggtggcggc cagggagctg cgttaccggt ggtttgggga
     3481 gttcgtcggg agtgggcggg tacggtggat cgtgacggcg catcatctcg acgacaatat
     3541 cgaaacgatg ctgatgaatt ttttcaaggg gacgggtatt gcgggcctgc gggggatatt
     3601 gccgaagcag ggcaatattg tccggccctt gttatttgcg cggaaggagg agctacgggc
     3661 ttttgccgtg gaggccggcc tggaatgggt ggaggacagt tcgaacgaat cggataaata
     3721 tacccgcaat tttttccggc accagcttat cccgctggtg gagcaggtgt atcccgcggc
     3781 gtctgccaac ctggcgtata accttggccg tttccgggag gtggaggcgt tgtaccggca
     3841 atcggtggag ggatggaaga aaaaactact cgagtataag ggtaatgagg tacatatccc
     3901 ggtggagaag ctgcggaagg cggcgccgct ggcgacgatc gtgtatgaga tcatcgagcc
     3961 ctatgggttc tcaccgcagc aggtggggga ggtgatggcg ttgatggaca gcggatcggg
     4021 gaggtttgtg cgggcgcggg acttccgcat cctgaagaac cggaactggc tgatcattac
     4081 gccgctgaca gaggagcagg cgacgaatat ccttgtcgag gaggggagag cggcggtgag
     4141 ttgtgcggag ggggtgttgc ggctggcgcg gatggatgcg gcggggatgg gtgcgctgga
     4201 ccaggggccg tcggtagcgt tgctggatgc ggggaagctg agttttccgt tgttgttgcg
     4261 ccggtggaag gcaggagatt atttttaccc gcttgggctg aggaagaaga agaaagtggc
     4321 ccgattcctt atcgacagca aggtatcgat tgctgagaaa gagcgggtat gggtgttgga
     4381 gatggataag aagatcctgt gggttatcgg ccggcggatc gacgaccgct ggaaggtggg
     4441 tacg
//
LOCUS       DATGFG010000048         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1476355, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000048 DATGFG010000000
VERSION     DATGFG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476355"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..254)
                     /locus_tag="VKQ52_00950"
     CDS             complement(57..254)
                     /locus_tag="VKQ52_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85767.1"
                     /translation="MTYLEVHSDRGVQRWRIQGLPAGKTEEDYLMRIEVLNLALAFFE
                     ENMSYAYDNPTVFIVHQSKFD"
     gene            complement(607..936)
                     /locus_tag="VKQ52_00955"
     CDS             complement(607..936)
                     /locus_tag="VKQ52_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85768.1"
                     /translation="MRIDEFDRIYRAGFLNLANVYLADYENGVFKLEMLNTLPVPRKV
                     VLTVYFHLVSSKELIPIEQLEKGVKAKLWKLCKPFTLGLNADGCRDIARCYLTLDHLL
                     AKKEAGE"
     gene            complement(933..1517)
                     /locus_tag="VKQ52_00960"
     CDS             complement(933..1517)
                     /locus_tag="VKQ52_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85769.1"
                     /translation="MLMATKKTFEKPTIDQLKAHMMEKMKDWPEAFCEYYAEKFWNSY
                     EKTGWRLSAGKGGPMKSWTAAFASNWKDLSYEIDRKKLAEAQAQEKARQARLIQREAK
                     LPATAVEIGAELVDGLMSEYMAHPTLVTKDRLAACYEWIKKNLRVKLSKEQTQIVIGI
                     AKDDEAKAKATVVEWVFEMLSCQGKTFKRLIEQV"
     gene            complement(1812..2399)
                     /locus_tag="VKQ52_00965"
     CDS             complement(1812..2399)
                     /locus_tag="VKQ52_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85770.1"
                     /translation="MISTLPQGLLTRRMNEINEMNTKAADRLLQKHGFDVEGNRARNT
                     TTRPSHKKKKAISKHRIQRLRVRERMKKSKWLVRQRQEADRSAGEKRIEVFLMENNIR
                     FVAEHSTTYCYSRKTNHLLFFDFWLPDHNMVIEFDGIHHFKPIYGDEKLAEQKYKDKT
                     KNQYCRKRGIRMLRIPCFVKDVEKAICIFFDRYGL"
     gene            complement(2416..>3184)
                     /locus_tag="VKQ52_00970"
     CDS             complement(2416..>3184)
                     /locus_tag="VKQ52_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HLZ85771.1"
                     /translation="GFGYLGMKTIVTDVVRENNQTYRLGWTEQCKDGSKMGVGMPEYL
                     LLFRKPPSDNNNSYADLPVLKSKERYTRSRWQIDAHGFARSNGNRGLTAEDLIGLEHK
                     AMFRIFKDYTLTTVYDFEHHVRIGEALEASGKLPTSFMLLQPQSWSEDVWTDITRMLT
                     LNGSQWSNGKEMHLCPMQFDIADRVIEQMSNPGDIVLDPFSGLGTVPYRAILLKRFGV
                     GVELNPSYFVDSATYCRAAEQEVNMPTLFDALENGAA"
BASE COUNT          703 a          911 c          672 g          898 t
ORIGIN      
        1 tcactttgac gaattcgaac tcctcccgcg tgtaagatgg tactaccccc atagctttaa
       61 tcgaatttgg attggtgtac gatgaaaact gtcggattat cataggcata ggacatattt
      121 tcttcaaaga aggccagcgc cagattcaac acctcaatcc tcattaaata atcctcctcc
      181 gttttacccg ccggcagccc ctgaatccgc caccgctgaa cgcctctatc gctgtgtact
      241 tccaaatagg tcattggtca ttttttacgt gtcgcgatac cttgtggccg tgctctactg
      301 cccaggcatg atgcgactcg acgtaatcgt tacacggggt gcaggatgct ttatgataca
      361 taggatcaag gactacatct tttgcgcggc cctttacatg gtgcacgcaa gtcgcaatat
      421 gggtacaaac cggggatcga atctcgcaga acggatgctc atccagaaag ggtccgatta
      481 tcttacccaa agaccgatat tccttcacct tcttcttcgt ccttggccga atgcggctcc
      541 atttcttttt agacttctta tcctttttac cggcctcgac cttcagcttc cggatattct
      601 cgatactcat tcccccgcct ccttcttcgc caacagatga tccagcgtta ggtaacatct
      661 ggctatatcc ctgcagccat cagcatttaa acccagtgtg aacggcttgc acaacttcca
      721 tagctttgcc ttcaccccct tctccagttg ttctatcggt atcagctctt tagatgaaac
      781 caaatggaaa tataccgtca gcacgacttt acggggtacc ggaagggtat tcagcatttc
      841 caatttaaac accccatttt catagtcggc cagataaaca ttggccaggt tcaagaagcc
      901 agcacggtaa atcctatcga actcatcgat cctcatactt gctcaattag tcgtttgaat
      961 gtcttaccct gacagctgag catttcgaac acccactcta caaccgtcgc tttagccttt
     1021 gcttcatcgt ctttcgcgat tccgatcaca atttgcgtct gctccttgct cagtttcacc
     1081 cgcaggttct tctttatcca ctcatagcag gccgccagcc gatcctttgt gaccaaggtc
     1141 gggtgagcca tatattccga catcagcccg tccaccaact ctgcgccgat ctccaccgcc
     1201 gtcgccggga gtttcgcttc ccgctggatc aacctggcct ggcgggcctt ctcttgggct
     1261 tgagcttcag ccagtttctt gcggtcgatc tcatagctga ggtccttcca attggatgca
     1321 aaggccgccg tccaagattt catcggaccg ccctttccgg ctgataggcg ccagcccgtt
     1381 ttttcatagc tgttccagaa cttttcggcg tagtattcac agaaggcctc cggccagtcc
     1441 ttcattttct ccatcatgtg cgccttgagc tggtctatcg ttggcttctc aaatgtcttt
     1501 tttgtggcca tcagcatttt gcttttgatt ggatattcat agtctatgta tactttctac
     1561 tctttattta agccccagat tggttgcttt cgagaacacc tttccctaaa agggtgatgg
     1621 taaaacatta acatctaccc ttccaggaaa ggcgcgtatt aagccccgag tcgtgtagct
     1681 cccattcgca ccccgccgct gaacggacgg cccagataag aatatccagg attcccctgt
     1741 cgcctcctta aacttcccct cttggggtaa acataggtgt gggtcattag ccccactgtc
     1801 cgacaggtaa cctataaacc gtatcgatcg aaaaaaatac agatcgcttt ctcgacatct
     1861 ttgacgaagc atggaattcg cagcatacgt atcccccgct tcctacagta ctggtttttg
     1921 gttttgtcct tgtacttctg ttcggccagt ttctcatcgc cgtaaatcgg cttaaaatgg
     1981 tgtatcccgt caaattcgat caccatgttg tggtcgggta gccagaaatc gaagaacaaa
     2041 aggtggttcg tcttccggct atagcaatat gtcgtgctgt gctcggccac aaaccggata
     2101 ttattctcca tcaaaaacac ctcgatcctc ttctcccccg cgctccggtc cgcttcctgc
     2161 cgctgtctga ccagccattt agacttcttc atcctctctc gcacgcgcag ccgttgtatc
     2221 ctatgcttgc tgatcgcctt cttctttttg tgactcggcc tagtcgttgt gttacgagcg
     2281 cggtttccct cgacgtcaaa gccgtgcttt tgaagtaacc tatcagctgc ttttgtattc
     2341 atttcgttta tttcgttcat tcgccgcgta agcaatcctt gcggcagggt tgaaatcatt
     2401 aagacccctt ttcaactaag ccgctccgtt ttctaacgcg tcaaacaacg tcggcatatt
     2461 cacttcctgt tccgccgccc tgcaatatgt cgcactgtcg acgaagtagg agggattcaa
     2521 ctccactcca acaccgaaac gctttagcaa gattgcacgg taaggcactg tgcccaagcc
     2581 gctgaagggg tccaacacga tatcccccgg attactcatt tgctcaatta ctcgatcggc
     2641 gatatcaaac tgcatagggc acaggtgcat ctccttccca ttactccatt gagacccatt
     2701 tagggtcagc atacgggtga tatcggtcca tacatcctcc gaccaacttt gcggctgaag
     2761 gagcataaac gaggtaggta gtttgccgga ggcttcgagc gcctcgccga tccgcacgtg
     2821 atgctcaaag tcatagacgg tggtcaatgt gtaatctttg aagatgcgaa acatagcctt
     2881 atgctcgaga ccgatgagat cctcggctgt caatccacgg ttgccgttgc tgcgagcgaa
     2941 accatgggca tcgatttgcc agcgggacct ggtgtatctt tccttcgact tcaggaccgg
     3001 caagtcagca taagaattgt tgttgtcaga aggtggtttt cggaatagga gcaaatactc
     3061 cggcataccc acgcccattt ttgagccatc cttacattgc tcggtccatc ccagccggta
     3121 agtctggtta ttctcccgta ccacatcagt gacaattgtt ttcataccca gatatccgaa
     3181 cccg
//
LOCUS       DATGFG010000049         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1592475, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000049 DATGFG010000000
VERSION     DATGFG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592475"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            533..694
                     /locus_tag="VKQ52_00975"
     CDS             533..694
                     /locus_tag="VKQ52_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85772.1"
                     /translation="MKNIVNELRGGDRCSIGKVDAIISEVSSRAKFDELFECLFNADY
                     LVTIRAADD"
     gene            complement(748..>3098)
                     /locus_tag="VKQ52_00980"
     CDS             complement(748..>3098)
                     /locus_tag="VKQ52_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ85773.1"
                     /translation="VCLLIVLYVTDELSYDGYNLNADRIYRVDADSYVNNTRFDAATI
                     SNFLGPTLVATYPKIQQMVRFRHPGDLLVRKGNDHVVEHHFTFADSTIFKVFTLPMIA
                     GDPNTALNNPHSIVIDESAARRYFSSTDVIGRTLEVGTDNIPLKITGVIRDMPEQSHF
                     HFSFIRPIRESNTFNDPDDNAWLANNHYTYILAQPGTTRAEVQKDVNAVVNLHIGRAI
                     QELVHTSMADLEKAGNHFRYRITPLTDIHLHSNKSYELEANGNIQFVYIFSVIAVLIL
                     LIACVNFMNLSTARSANRAKEVGIRKVAGSTKGHLIIQFLTESVLLSLFSLVLALGIA
                     VLLLPMLNQLAGKSLHPDVLFSGRFLPILLLLVLLVGCLAGSYPAFYLSSFQPIHVLK
                     GKVAAGFKSSWLRNSLVVFQFFISIGLIVSTLVIYRQLHYIRNKEVGFNRDQVLVIHD
                     TWSLGRDGTTNLRKNLLTLSGVTDATVTGDLPSVGGDQYSQPGWFRDASLDARKAIFM
                     TTLAVDDHYVPTLGMQIVKGRNFDFAQFPTDSTGIILNEAAVAMLGVKDPLHLIFWNK
                     PHGFPARPFHVVGVVKDFNYNSMHDQIRPLVMEVNTINWPGVAVRFHTNDVFSLVRQV
                     ESKFHAAKQGLPFSYSFMDNDFDKLYHADQQTGQIFITFAVLAILIACLGLFGLVTYA
                     AEQRTKEIGIRKVLGARVTGVVGLLSKDFTILVGIAALIAFPVSWWAMYKWLETFAYR
                     TEISWWIFLVAGAVALAIALLTVSIQTIRAALANPVKALRSE"
BASE COUNT          751 a          698 c          970 g          679 t
ORIGIN      
        1 agcttttgct ttgtctccga cgtatgggta gattctgatt ttcgacagga aaagatgacc
       61 aggaaaatgc ttgcggcgag taggagatat tttttcataa cttaaagttt gatcacaaat
      121 ctaacgtgtg tgagtgcaaa aaatttgcat cacgaaatat cgacgtcgct gcttacccat
      181 gctcaccttt ctcacgtcag gtatcctaat gggttatatt tatttgttca aaagaaactg
      241 cgcgatgata taaatgccaa aaccattttt tttagcacat cagtcttcct cttttttttg
      301 atcaaaaatt caggaaaact atcgctcgac aataaacgga gaacctggca gaactatatc
      361 gacccaaagg acccgctgcc agagcgggtg ccggctagaa tcacccatcg ggttgatttg
      421 tctcattgtt tcaaagcctg gcgctgaata gctttatcta aaacatgaaa aatatcaaaa
      481 gaagtttgct atttggcttt acaatggggc ttttgctgga gaaaaagctg gtatgaaaaa
      541 catagtcaat gaactgcggg gtggcgaccg ctgttccatc ggcaaagtgg atgcaataat
      601 ttccgaagta agcagccggg cgaaattcga tgagctgttc gaatgcctct ttaatgctga
      661 ttacctcgtg actatacggg ccgcggatga ttgataaacc cgctcctgga atttccacgc
      721 gaggtcacct tgttcaacat tcaccgatca ttcgctgcgg agggctttta cggggttagc
      781 gagggccgcg cggatggtct gaatactcac cgtcaatagg gcaatggcga gggcgacggc
      841 gccggccacg aggaagatcc accagctgat ctccgtacga taggcaaacg tctcgagcca
      901 tttgtacatg gcccaccacg agacggggaa ggcgataagc gcagcgatcc cgacaaggat
      961 cgtgaagtcc ttgctgagga gaccgacgac gccggtgacc cgggcgccga ggaccttgcg
     1021 gatcccgatc tccttggtgc gttgctcagc agcataggta acgaggccga aaagcccaag
     1081 gcaggcaatg aggatggcga ggacggcgaa ggtgatgaag atctggccgg tctgctgatc
     1141 ggcgtgatac aatttatcaa agtcgttatc catgaaggag tagctgaagg gcagcccctg
     1201 cttggcggca tggaacttac tttccacctg ccggaccagg ctgaagacat cattcgtgtg
     1261 gaagcgtacg gccacgccgg gccagttgat ggtgttgact tccatgacga gggggcggat
     1321 ctggtcgtgc atggaattat aattgaaatc ctttacgacg ccgacgacat gaaaggggcg
     1381 ggcggggaat ccgtgcggtt tattccagaa gatcaggtgg aggggatcct tcacgccgag
     1441 catcgctacg gcggcttcat tgaggatgat tccggtagag tcggtaggaa attgcgcgaa
     1501 gtcgaagttg cggcctttga cgatctgcat gccaagggtg gggacataat ggtcgtcgac
     1561 agcgagcgtg gtcatgaaga tcgccttcct ggcgtcgagc gaggcatccc ggaaccagcc
     1621 tggctgacta tactgatcgc cgccgacgga ggggaggtcg ccggtgacgg tggcgtcggt
     1681 gaccccggaa agggtgagca ggttcttgcg aagattggtc gtgccatccc ggccgagcga
     1741 ccaggtatca tgaatgacga gtacctgatc ccggttaaag ccgacttcct tatttcggat
     1801 atagtgcagc tggcgataga tgacgagggt gctgacgatg aggccaatag aaataaaaaa
     1861 ttgaaagacc accaggctgt tacggagcca gctgctcttg aagcctgcgg ccaccttccc
     1921 tttcaataca tgaatcggct gaaaggagga aaggtagaag gcgggataac tgcccgccag
     1981 acaaccgacc agcagcacca ggaggaggag gatgggcaga aagcggccgc tgaagagcac
     2041 gtcgggatgg agcgatttgc cggcaagctg attgagcatc ggtagcagga ggacggcaat
     2101 gccaagggcg aggacgaggg agaagaggct gagcaggacg gactcggtga gaaactggat
     2161 gatgaggtgc ccttttgtcg agccggcgac cttgcggatg cctacttcct ttgcgcggtt
     2221 ggcgctacgg gcggtgctga ggttcatgaa attgacgcag gcgatcagga ggataagtac
     2281 ggcgatgacg gagaagatgt aaacgaactg gatattgccg ttggcttcga gctcgtaaga
     2341 cttattggaa tggaggtgga tgtcggtgag gggggtgatg cggtaccgga aatgattacc
     2401 cgctttctcg aggtcggcca tcgacgtgtg gaccaattcc tggatcgccc ggccgatatg
     2461 gaggttcacg accgcgttta catccttttg gacctcggct cgcgtggtgc cgggttgggc
     2521 aaggatatag gtataatgat tattagctag ccaggcattg tcgtcgggat cattaaaggt
     2581 attggattcg cggatggggc gaataaagct gaaatgaaaa tgggactgct cgggcatgtc
     2641 ccggatgacc ccggtgatct tgaggggtat attgtcagta ccaacctcca gcgtgcgtcc
     2701 gatgacgtcg gtactgctga aatagcggcg ggcggcactt tcatcgatca cgatggaatg
     2761 agggttgttg agggcggtat tggggtcgcc ggcgatcatg ggcagggtga agaccttgaa
     2821 gatcgtcgag tcggcgaagg taaagtgatg ctcgacgacg tgatcgttgc ccttcctaac
     2881 cagcaggtcg cccgggtggc ggaatcggac catctgctgg atcttcggat aggtagcgac
     2941 gagggtgggg ccgagaaagt tggagatggt ggcggcgtcg aacctggtat tattgacgta
     3001 ggaatccgca tcgacccgat agatgcggtc ggcgttgagg ttgtagccgt cataactgag
     3061 ctcgtcggtg acgtacagga cgatcagcag gcagacac
//
LOCUS       DATGFG010000050         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_337178, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000050 DATGFG010000000
VERSION     DATGFG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337178"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..549
                     /locus_tag="VKQ52_00985"
     CDS             28..549
                     /locus_tag="VKQ52_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HLZ85774.1"
                     /translation="MQNKAKTAKESLIVMTELVLPNDTNVFGNLMGGRLMYWMDIAAA
                     LAAGKHCNAPVVTASVDNISFENPIKLGNVVHIEAKVSRAFNSSMEVHMKVWGEDHTQ
                     QYKYKSNEAYYTFVALDPNRRPRPVPELIAETEEEKKLYDGALRRRQLRLILGGKMKP
                     EEAGELKAFFIKS"
     gene            813..1499
                     /locus_tag="VKQ52_00990"
     CDS             813..1499
                     /locus_tag="VKQ52_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85775.1"
                     /translation="MNSQKQFINCCYLLLLLTLSTHPATAQDGPDQDSRLYNGYEYIR
                     NGTHAQGFPFFGIDSLQPGTLVYDGILHRDIAMEYDLVLDKLVIPDYTGKALISLISE
                     KVDRFTIGPHAFRYFPAQPTTSSLPKQGFYEELLGRDSVALLARREKKLVFPSSQDEQ
                     AKYEQSNSHFLLIGNRSARVADESSLLDMLKDKKDMLKKYIRDNHIRFKKDLENALIA
                     TTSYYLKLRN"
     gene            1501..>2361
                     /locus_tag="VKQ52_00995"
     CDS             1501..>2361
                     /locus_tag="VKQ52_00995"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ85776.1"
                     /translation="MHTRLHYILLYGLSLITGIQALGQEQKRVTGNFEGYTFSRLADR
                     LEALTGYHFYYDPTDLDSLSIDMTVNKATIPQILDHLFQNTDFHYAIDSTGRVFITRH
                     VAILTSLPRYLGGATRLQDSIKNILPAEQDQPERSGLKKVLVENQLLDIGNKAGRPGG
                     KATIAGYVRNDKTGEPIVGANLYTDTTTPAVTTDQFGYYSLTLPKGRHIIRISYAGMK
                     DTRRQINLHSDGKLDVDMQDAVTTLKTVIVSAEKTSNTQSVQMGVNRLNIKQIKQVPV
                     VFGETDVMKVV"
BASE COUNT          656 a          796 c          481 g          428 t
ORIGIN      
        1 atcgaaccat aggtctatat ttgcagtatg caaaataaag caaagacggc gaaagaatcc
       61 ctaattgtca tgacggagct cgttctcccc aatgatacca atgtgttcgg caacctcatg
      121 ggcggccgcc tcatgtactg gatggatatc gccgccgccc tggccgcggg taaacattgc
      181 aacgcccccg tcgtcacggc ctccgtcgac aacatctcct tcgaaaaccc catcaaacta
      241 ggaaatgtcg tgcacatcga agcaaaggtc agccgcgcat tcaacagttc catggaagtc
      301 catatgaagg tatggggcga agaccatacc cagcaataca aatataaaag caacgaagcc
      361 tactacacct tcgtagccct cgaccccaac cgccggcccc gccccgtccc cgaactcatc
      421 gccgaaacag aagaggaaaa aaagttatat gatggcgccc tccgccgccg ccaattgcgg
      481 ttgatcctcg gcgggaaaat gaaacccgaa gaagccggcg aactaaaagc cttcttcatc
      541 aaaagctgaa caacgcaaac actacccaac gcaaaaacct actcaaccca aacactaccc
      601 agcgccaaaa cctaccctgc gcaaaaacct accccaacac aatcacctac cccaacccga
      661 aagcccctgc gctcacgtcc caaaacaact gccgcagacc caaactttat ttcttaaaca
      721 cacccatttg ttctatattt agaaagcgta cattatacac aatggcgcgc ttctaatatc
      781 ctcccctcgt cacacgccaa agcttgtcat aaatgaattc ccaaaagcaa ttcatcaact
      841 gctgctatct gcttttactc ctgaccttgt caacccatcc ggccaccgcc caggacggcc
      901 cggatcagga cagccggctc tacaacggct atgaatatat ccgcaacggc acccacgcac
      961 aaggcttccc cttcttcggc atcgacagcc tgcaacccgg caccctcgtc tacgacggca
     1021 tccttcaccg ggatatcgcc atggaatacg atcttgtact cgataaactg gtcatccccg
     1081 actacacagg caaggccctg atcagcctca tcagcgaaaa ggtcgatcgg ttcaccatcg
     1141 gtccccatgc ctttcgctac ttccccgcac agccaaccac ttcatcactg ccaaaacaag
     1201 gtttttacga ggagctcctc ggccgggact ctgtcgcact cctcgcccgc cgcgagaaaa
     1261 agctcgtgtt cccctccagc caggacgagc aggcaaaata tgaacaaagc aattcccact
     1321 tcctgctcat cggtaaccgc tccgcccggg tggccgacga gtcatccctg ctcgacatgc
     1381 tcaaagacaa aaaggacatg ctgaaaaaat atatccgcga caaccatatc cgcttcaaaa
     1441 aagacctgga gaacgcactg atagcgacta cgtcgtatta cctgaaattg agaaactgac
     1501 atgcataccc gcctccatta tatcctgctc tatggccttt ccctcatcac cggcatacag
     1561 gcgctgggac aggaacagaa gcgggtcaca ggcaatttcg aaggatacac cttctcccgc
     1621 ctcgccgacc ggctggaagc gctcaccggc tatcatttct actacgaccc caccgatctc
     1681 gacagcctct ccatcgatat gaccgtcaac aaggcaacca tcccccagat cctcgatcat
     1741 ctcttccaga ataccgattt tcactacgcc atcgactcca ccggccgggt cttcatcacc
     1801 aggcacgtcg ccatcctcac ctccttgccc aggtatctcg gcggcgccac ccggctgcag
     1861 gattccataa agaacatcct tcccgcagaa caggaccagc cggaacggtc aggattgaaa
     1921 aaagtactcg tcgagaacca attactcgac atcgggaata aggccggccg gccgggcggc
     1981 aaagcaacca tcgccggcta tgtcagaaac gacaaaaccg gcgaaccgat cgtcggcgcc
     2041 aacctctaca ccgataccac gacacccgcc gtcacgaccg accagttcgg ctattactcc
     2101 cttaccctgc ccaaaggccg tcacatcatc cgcatcagct acgccgggat gaaagatacc
     2161 cgccgccaga tcaacctcca ctccgacggg aaactggacg tcgatatgca ggacgcggtc
     2221 accaccctga agaccgtcat cgtctccgca gaaaagacct cgaataccca aagcgtgcag
     2281 atgggagtta accggctcaa cataaaacaa ataaaacagg tgccggtcgt cttcggcgaa
     2341 accgacgtga tgaaagtcgt a
//
LOCUS       DATGFG010000051         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1019978, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000051 DATGFG010000000
VERSION     DATGFG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019978"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1168
                     /gene="sigJ"
                     /locus_tag="VKQ52_01000"
     CDS             263..1168
                     /gene="sigJ"
                     /locus_tag="VKQ52_01000"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HLZ85777.1"
                     /translation="MSMEPKAELFLEYKALLFSMAYNMLGNIDAAEDIVQDVFVKWME
                     TETEAVKNRKAFLVRMVTNASINHLTSARARREKYVGIWLPEPLPDSGTPAFITRIES
                     YHALSIGLLVLLEKLTPQERAIFLLKEIFSYDYDELAEIFGKTTDNCRQILKRAKEHL
                     GKDTRRFKVDMKVHERILSNFLEAVTAGSMEELIHLLKEDILLFADGGGAVFALQQQR
                     ITAFPKPIAGRENIVRVLLSLVPKFRTSVPDYRQETTLVNGLPTLVIYSGSTAVSLVA
                     LETDGEQIRNIYVQTNPDKLKHFKR"
     gene            1194..1667
                     /locus_tag="VKQ52_01005"
     CDS             1194..1667
                     /locus_tag="VKQ52_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85778.1"
                     /translation="MTQQTFLNLDLVLHITGFTMMAGIILADFAVSRRLNRYLITDKP
                     RAVSIMESVAGFPRLIGIGGAILLVTGIAMVVVFKGVVAQMLWFKIKMVIVLLVALNG
                     SVVLRRNSNRLKLMLQSNDDRQNGNILALKQRLGVYHGIELLLFLVIFVLSIFRF"
     gene            complement(1688..>2016)
                     /locus_tag="VKQ52_01010"
     CDS             complement(1688..>2016)
                     /locus_tag="VKQ52_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85779.1"
                     /translation="FYNGQPRMRKRQLGWRPDHRDHRKTLGQCRLHGLPADPAIGAKH
                     QDHTSLPAATPLPAAALPSATPLPSANTLPSAALPAAPLSANPVPSAATTPLPAATPL
                     PTGICE"
BASE COUNT          464 a          468 c          626 g          458 t
ORIGIN      
        1 gccggcgccc aggagctgtt tgtcatcctg gtaaggggcc aactcgttta cgggggtggt
       61 tttttggatc gtcatgctaa ggccggcaac ggctatgagc gtagctgcga cgatcgaaag
      121 tttcgggatc ttcatttttg agtgttttac aagttaagag ggtgtaaaag aagggcgata
      181 aaacaagatt tggagaaaaa gtgctttatg gaaatattat cattttgacc ctgtcacaaa
      241 taaaggggtt tatttgtctt atatgagtat ggaaccaaaa gcagagctat tccttgaata
      301 taaggccctt ctcttttcga tggcctataa catgctgggc aatatcgatg cggcggagga
      361 catcgttcag gatgtcttcg tcaagtggat ggagacggaa accgaagccg taaagaaccg
      421 taaggctttt cttgtacgga tggtcaccaa tgccagtatc aatcacctga ccagcgcccg
      481 ggccagaagg gaaaaatatg tcggtatctg gctgccggag cccctcccgg acagcggtac
      541 ccccgccttc atcacccgga tagaaagtta tcatgccctg tcgatcgggc tgctcgtgtt
      601 gctggagaaa cttaccccgc aggagcgggc cattttcctg ttaaaggaga tcttctccta
      661 tgattacgat gaattagcgg agatctttgg caagacgacc gataactgcc ggcaaatatt
      721 aaagcgggca aaggagcacc tgggcaagga tacccggcga ttcaaggtgg atatgaaggt
      781 gcacgagcgg atcctgagca atttcctgga agccgtcacg gccggcagta tggaagaact
      841 gatccacctg ctcaaggagg acattctttt gtttgccgat ggcggcgggg ctgtctttgc
      901 ccttcagcag cagcggatca cggctttccc gaagccgatc gccggccgcg agaatatcgt
      961 ccgcgtcctg ctcagtcttg tccccaaatt ccggacctcc gtcccggatt accgccagga
     1021 gaccacgctg gtgaacgggc tccctaccct tgtcatttat tccggaagca ctgccgtcag
     1081 ccttgtcgcc ctcgaaacgg acggggagca gatacgaaat atctatgtac agacgaaccc
     1141 agataaattg aagcatttta agaggtaacc gttcggattt aaacaccatt tatatgacac
     1201 aacaaacctt tctcaacctg gatctcgtat tgcatatcac gggctttacc atgatggcgg
     1261 ggatcatcct ggcggatttc gccgtcagca gaagactgaa tcgctatctg atcaccgaca
     1321 aaccaagggc tgtgagcatc atggaaagtg tggccgggtt cccgcgactg atcgggatcg
     1381 gcggggccat cctgcttgtc accggcatcg ccatggtcgt cgttttcaaa ggcgtcgtcg
     1441 cgcagatgct ctggttcaag atcaagatgg tgatcgtact gctcgtagcg ctgaacggat
     1501 cggtggtatt gcgaaggaac agcaatcgtc tgaagttgat gcttcagtcc aacgatgacc
     1561 gtcagaacgg aaatatcctg gcgctgaagc agcggcttgg cgtttatcac ggcatcgaac
     1621 tgttgttgtt ccttgtcatt tttgtactga gcattttccg gttttaatta tggaaaggaa
     1681 aaagtattta ttcgcagatg ccggtgggga ggggagtggc agcggggagg ggagtggtgg
     1741 cggcggaagg gacgggattg gcggagagag gggcggcggg gagcgcggcg gaagggaggg
     1801 tattggcgga ggggagagga gtggcggaag ggagagcggc ggcggggaga ggagtggcgg
     1861 cggggaggct cgtgtgatcc tggtgcttgg cgccaatggc gggatcggca ggcaggccgt
     1921 ggaggcggca ctggccgagg gtcttccggt gatcgcggtg gtccggacgc catccaagct
     1981 gtcgcttacg catccgcggt tgaccgttgt aaaagg
//
LOCUS       DATGFG010000052         4104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_790026, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000052 DATGFG010000000
VERSION     DATGFG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790026"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VKQ52_01015"
     CDS             <1..678
                     /locus_tag="VKQ52_01015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLZ85780.1"
                     /translation="EVPILCYHQVRDWKATDSKASKDYIMPPADLKAELKMLHDSGYH
                     TILPDQLYAYLTKGAALPSKPVMLTFDDTDEDQYTVAWPEMKKYGFKGVFFIMTISLN
                     KLPHYMTKDQVKELYAAGNVIGSHTWDHHNVKKYQGKDWEIQIDKPTKQLEAITGSPI
                     HYFAFPFGLWNRAALPELRKRGFIMAFQLADKMDPTDPLMTVRRIIVGGGWSLKTFNY
                     AIHRDFH"
     gene            complement(781..1959)
                     /locus_tag="VKQ52_01020"
     CDS             complement(781..1959)
                     /locus_tag="VKQ52_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLZ85781.1"
                     /translation="MSEKSGIYKKKIIRELYFAGNLSCSDISDRTSTSLPLATKMLGQ
                     LVEEKYVMPMGYAPSTGGRRPQSYSLRPESFFIVSVAMDQLSTRIVIRDMHQKQVTAI
                     RKFEFPLAAKDATPDRLAGLINEFILRSGIGSTQIAGIGIGMPGFVDSHEGINYSFLK
                     PAEKGLAQYLTEHTGIQTFIENDSGLIALAEFRFGLARDMRNVLVINLGWGVGLGMIL
                     NGEYFRGHNGFAGEFSHLPIYSNNKLCSCGKSGCLETEASLLELIAKAEEGIRNGRLT
                     SLSHQLPAKDPDMAAQAILSAAQDGDKFAVGLVSEAGYTIGRGIAILIHLLNPEMIVL
                     SGQGASAGKIWQAPIQQALNEHCIPRLASNTRIEVSALGNDAELIGAAALVVEGYYSA
                     "
     gene            complement(2245..3477)
                     /locus_tag="VKQ52_01025"
     CDS             complement(2245..3477)
                     /locus_tag="VKQ52_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HLZ85782.1"
                     /translation="MPAVKKYHFTLLLIPAIFLLSAHWKPPAPTPQDRTPNPYDTSAF
                     IGSAACQSCHKDIYAAYIHTAHYLDSRPAAEAFIKGSFKGDKGRFVLNEHMEVRMETK
                     AGRFYQTALLNGNPFESEAFGIVIGSGRKGQTYLYWDGNRLFQLPVSYFVPLDSWCNS
                     PGYPRNMVYFGKQVHGQCLECHGTHAKMAELADLGTVFDSTSILYGIDCERCHGPAAQ
                     HVAWQAAHPTERTAKYIINTRHLSRQQRLDACALCHSGFRDEKLPSFSFTVGDTLDNY
                     STATYDTSATAALDVHGNQYGLLTASKCFRLTDRLDCSSCHDPHVNEHAKMDVYSQRC
                     VNCHNGNSLHSCSFPPVRTMGLTNNCIDCHMPALPSNAITLQLSGQAQLVHDLVRTHR
                     VAIYPDATKAYLEKIKRR"
     gene            complement(3481..>4104)
                     /locus_tag="VKQ52_01030"
     CDS             complement(3481..>4104)
                     /locus_tag="VKQ52_01030"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HLZ85783.1"
                     /translation="DVYRTVNTLSDEQKAVANFWDCNPFAVTTEGHLSFATKKISPGG
                     HWIAIVTTVSRQQHRDIIETAAAYTITSIAIFDAFISCWDEKYRSNVVRPETYIDANI
                     DEGWRPLLQTPPFPEYTSGHSIVSMVSATVLATYFGEDCAFSDDTERQFGLPERHFVS
                     FLQAAQEAAVSRLYGGIHYRAAIEAGQVSGRQIGEWVLQKIKLKKAG"
BASE COUNT          863 a          998 c         1301 g          942 t
ORIGIN      
        1 gaagtgccga tcttgtgtta tcaccaggtg agggattgga aggcgaccga ttccaaggct
       61 tcaaaggatt atatcatgcc gccggccgat cttaaggcgg agctgaagat gttgcatgat
      121 agcggctatc atacgatctt acccgaccag ctatacgctt atttgacgaa gggggctgcg
      181 ttgccgagca agccggtgat gctgaccttc gacgacacgg atgaagacca gtataccgtt
      241 gcctggccgg agatgaagaa atatggcttc aagggggtgt tctttatcat gaccatcagc
      301 ctgaacaagc tgccccacta tatgacaaaa gaccaggtga aagagctgta cgccgccggc
      361 aatgtgatcg gcagtcatac gtgggaccat cataatgtaa agaaatacca gggtaaggat
      421 tgggagatac agatagacaa gccgaccaag cagctggaag cgataacggg ttcaccgatt
      481 cattattttg cttttccgtt cgggctgtgg aaccgcgcgg cgttgccgga gttgcgtaaa
      541 cgcggtttta tcatggcgtt ccagctggcg gataagatgg atccaaccga tccgttaatg
      601 actgtgcggc ggatcatcgt cggaggcgga tggagcctga agacgtttaa ttacgctatc
      661 catagagatt ttcattagag cggggacggg ggcgacgtga ggacgggggc cggggtgagc
      721 ggcgacgggg cgacgaccat ggaggtgaag acgggggcga cgtgagggcg acgacgatgg
      781 tcaggctgaa tagtagcctt ccaccaccaa tgcggcggca ccgatgagct ctgcgtcgtt
      841 tcccaatgcc gagacttcga tacgcgtatt ggaagccagg cgtggtatgc aatgttcatt
      901 caacgcctgc tggatgggcg cctgccagat ctttccggcg gatgcaccct gcccgctgag
      961 tacgatcatc tcgggattga ggagatgaat gagaatggcg atccctcttc cgatggtata
     1021 gccggcctcc gatacgaggc cgacggcaaa tttgtcaccg tcctgagcag ccgaaaggat
     1081 ggcctgggca gccatgtcgg ggtcctttgc cggcaactga tgactgagag atgtcaggcg
     1141 gccgttccgg ataccttctt cggcttttgc aatcaactcc agcagagagg cttcggtctc
     1201 aaggcagccg ctcttgccgc aactgcataa tttgttgttc gagtagatag ggagatggct
     1261 aaactctccc gcaaatccgt tgtgtccccg gaaatactcc ccattgagga tcatgcccag
     1321 tcccacaccc catcccaggt tgatgaccag gacgttcctc atgtcgcgcg ccagtccgaa
     1381 ccgaaactcc gccagggcga tgaggccgga gtcgttctcg ataaaggtct gaatgcccgt
     1441 gtgctcggta agatactgcg caagtccttt ttctgccggc ttcaggaagc tgtagttgat
     1501 cccttcgtgg gagtccacga aaccgggcat cccgatgccg atgccggcga tctgggtgct
     1561 tccgatgccg gaacgcagaa tgaactcgtt gatcaggccg gcgagccggt cgggtgtggc
     1621 gtctttggcg gccagcggga actcgaattt cctgatcgcc gtcacctgct tttggtgcat
     1681 atccctgatg acgatgcggg tggagagttg gtccatcgcg acactgacga taaagaagga
     1741 ttcggggcgg agggaatagc tctggggacg ccggccgccc gtagaaggtg cgtagcccat
     1801 gggcatcaca tatttctctt cgacgagctg gccgagcatt ttcgtagcca ggggaagact
     1861 ggtgctggta cgatcactga tatctgaaca ggataagttg ccggcaaaat acagttctct
     1921 gatgatcttc tttttgtaga tcccgctttt ctcactcata ggaaagtatg cccattcatc
     1981 ttggacgtgt aaagttactt ttttcccgtg agcattgatc tgatggggca gagattattc
     2041 gcctgcgtgc tgattttgtg catttccgtg cagctgctgc gcagcttcgt gcaggaactg
     2101 ttcattttcg tacaggtact gttcattttc gtacaggtgt tgttcacttg cacgcagggg
     2161 ttagagggag gtggcggcgc tggggcaatc agtggtgttg ggagcggtcg ttggtgttgg
     2221 ggcagtcagc ggtgttgagg gcagtcagcg gcgttttatt ttttcgaggt atgccttcgt
     2281 ggcatcggga tagatggcga cccgatgcgt gcgaacgagg tcatgtacga gttgcgcctg
     2341 gccggacagt tgcagggtga tcgcgttaga ggggagtgcc ggcatatgac aatcgatgca
     2401 attgttcgtc aggcccatcg ttctcacggg aggaaaggaa caggagtgca ggctgttgcc
     2461 gttgtggcaa ttcacacatc gctgtgagta aacgtccatt ttagcatgtt cattgacgtg
     2521 agggtcgtgg caggaggagc aatcgagccg gtcggtgagc cggaagcatt tgctggccgt
     2581 gagcaggccg tactggttgc catggacatc aagagcagcg gtggccgatg tgtcgtaggt
     2641 tgccgtggag tagttatcga gggtatctcc tacggtaaac gaaaaagagg gcaacttctc
     2701 gtcgcggaac cccgagtggc agagggcgca ggcgtcgagg cgttgctgac ggctgagatg
     2761 ccgggtgttg ataatatatt tggcggtacg ttcggtggga tgcgcggcct gccaggcgac
     2821 atgttgcgct gcggggccat ggcaacgttc gcaatcgatg ccataaagga tgctggtact
     2881 gtcgaacacc gtccccagat cggccaattc agccattttt gcatgtgttc catggcattc
     2941 caaacactgc ccgtggacct gcttgccgaa ataaaccata ttgcgcggat atccggggct
     3001 gttacaccag ctgtcgagcg gaacaaagta cgaaacgggc agttggaaaa gccggttccc
     3061 atcccaatat aaataggtct ggcccttgcg gccggacccg ataacgatgc cgaatgcttc
     3121 gctttcgaaa gggttgccgt tgagcagggc cgtctggtaa aagcggccgg cctttgtttc
     3181 catccgcact tccatgtgtt cattcaggac aaaccggcct ttgtcgcctt tgaaagagcc
     3241 tttgatgaag gcttcggcgg cgggccggga gtcgaggtaa tgggcggtgt ggatgtaggc
     3301 ggcataaata tccttgtgac aagactgaca ggcggcggat ccgatgaatg cagaagtatc
     3361 gtagggattg ggggttcggt cctgtggcgt cggggctggc ggtttccagt gggcgctgag
     3421 gagaaagatc gccggtatca gcaagagggt gaaatgatat tttttaaccg ccggcatgta
     3481 tcatcctgct ttttttagtt tgatcttttg cagcacccac tcccctatct ggcggccgga
     3541 gacctgtccc gcttcgatgg cggcccggta atggatgccg ccatacagcc tggagacggc
     3601 ggcttcctgc gcggcctgca ggaaagatac gaaatgtcgt tccggcagcc cgaattgccg
     3661 ctcggtgtcg tcgctgaaag cgcaatcctc cccgaaatat gtcgccagca ccgtagcgga
     3721 gaccatcgag acgatgctat gaccgctggt atactcaggg aaggggggcg tctggaggag
     3781 gggacgccag ccttcatcga tgttggcgtc tatataggtc tcgggacgga cgacgttgct
     3841 acggtatttc tcatcccagc aactgatgaa ggcatcgaag atggcgatgg acgtaatggt
     3901 ataggcggca gccgtctcta tgatgtcgcg gtgctgttgc cgggacaccg tcgtaacgat
     3961 ggcgatccag tggcccccgg gggagatctt tttggtggcg aagctcaggt ggccttccgt
     4021 ggtaacggcg aaggggttgc agtcccagaa attggcgacg gctttttgct catccgacaa
     4081 ggtgttgacg gtgcggtaga catc
//
LOCUS       DATGFG010000053         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1245716, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000053 DATGFG010000000
VERSION     DATGFG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245716"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VKQ52_01035"
     CDS             <1..399
                     /locus_tag="VKQ52_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85784.1"
                     /translation="FSPDGKTIYFDWQIPKGQTFYLGAVDVQTGAEKKYAMTRDEWSI
                     HFNVSSDEKLFCGDGGDAGQVAKAMDGRWIYLFHPDGDHFVSERLVNMKDQFYRLEPN
                     VHFSPDNKWVIFRANFEGKEEVYAVECKKS"
     gene            413..>2890
                     /locus_tag="VKQ52_01040"
     CDS             413..>2890
                     /locus_tag="VKQ52_01040"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HLZ85785.1"
                     /translation="MLHPVARSLVRSIRLLVLLTLPFLTRAQLQWPAITAQTKPWTRW
                     WWMGSAVNRRDLTFNLEAYHRAGLGGLELTPIYGVKGYENQFVDYLSPQWMDLLSYTL
                     QEAKRLDLGLDMSTGTGWPFGGGPLIDSTYACRELFVKSWTVPGGATLPDSMTISFRQ
                     EPYVHTDGRPTTVDQLKEPVFANKDLQALALFQVRFPDAAPLRSLMAYSSAGAILDIT
                     DKVDATGRLRWTAPAGGNWTLYGLFLGWHGKMVERAAPGAEGNVIDHFSATALRKYLS
                     RFDTAFAGHDLASLRCFFNDSYEVDDSRGQSNWTAGFFDAFRSHRGYDLREHLPALFG
                     RDTKENNGRVLCDYRETISDLLLEQFTKPWHDWAHERRALIRNQAHGSPANILDLYAA
                     SDIPETEGQDILRYKFATSAAHVTGKRLASAETVTWLNEHFLSTWSDVKKALDGFFLG
                     GVNHVFYHGTAYTPESDPFPGWLFYASVHFTPNDPSWPDFAALNQYVARCQSFLQQGA
                     PDNDILLYYPIYDSWSESGAGNALLKHYDRMDPEFTNTGFKECAEYLQSHGYAFDYVS
                     DKQLQGPLPAQYRTILLPDCRYLPTDALQRLINLEKQGITVLVYKDLPSGPPGLGDLP
                     QRTTAFRQLLAKNGFAVGADLNTLLSGARLRRETLVDDSLGFIRRTYGNGNVYFLVNK
                     GSRSFTGWAPLAVKFTAAGIFDPITGEKGLAQVRDNAVYLQLRPGESCLVETNVAPRG
                     PLFAYYEPAGNPIPLKGDWQLSFLSGGPSLPKPVTLTQLAPWTDKTFSGTAKYSISFP
                     RPAGAPTAWLLDLGRVVRNAEISLNGQY"
BASE COUNT          590 a         1030 c          725 g          545 t
ORIGIN      
        1 ttcagccccg acggcaagac catctatttc gactggcaaa tacccaaggg ccagaccttc
       61 tacctcggtg cggtggacgt ccaaacgggc gccgagaaaa aatatgccat gacccgcgac
      121 gaatggtcca tccatttcaa cgtctcctcc gacgagaaac tcttctgcgg cgacggcggg
      181 gatgccggcc aggtcgcaaa ggcaatggac ggccgctgga tctacctctt ccaccccgac
      241 ggtgatcact tcgtgtccga acgactggtg aacatgaagg atcaatttta tagactggag
      301 ccgaatgtgc atttttcccc ggataataaa tgggtcattt tccgtgctaa ctttgaaggg
      361 aaggaagaag tgtatgcagt agaatgcaaa aaatcataaa ccagttcaac ccatgctcca
      421 tccggtcgcc cgttcattgg tgaggtccat ccggctcctc gttcttttga ccctgccctt
      481 cctgacccgc gcccaactcc agtggcccgc catcacggcg cagacaaaac cctggacgcg
      541 ctggtggtgg atgggcagcg ccgtgaaccg ccgggatctc accttcaatc tggaggcata
      601 ccaccgcgcc ggcctgggcg gtctcgaact gacacccatc tacggcgtaa aagggtacga
      661 aaaccagttc gtggattatc tttctccgca gtggatggac ctcctttcct ataccctgca
      721 ggaagccaaa cgcctcgacc ttggcctcga tatgtccacc ggaacgggct ggccttttgg
      781 gggtggcccc ctcatcgatt ccacctatgc ctgccgggaa ctcttcgtaa aaagctggac
      841 cgtgccgggt ggcgccacgc tcccggactc catgaccatc agcttccgcc aggaacccta
      901 tgtccatacg gacggccgcc ccaccaccgt cgatcaactg aaagaacccg tattcgccaa
      961 taaagacctg caggccctgg ccctctttca ggtgcgcttt cccgacgccg cgcccctccg
     1021 gtccctgatg gcctattcct ccgccggggc gatcctcgac atcaccgata aggtggatgc
     1081 cacgggccgc cttcgctgga cggcgcccgc cggcggcaac tggaccctct acggcctctt
     1141 cctcggctgg catggaaaga tggtcgaacg tgcggcgcca ggtgccgaag ggaatgtcat
     1201 cgaccacttc tccgcgacgg cgctccgcaa atacctgagc cggttcgaca cggccttcgc
     1261 cgggcacgac ctggcatccc tccgctgctt cttcaacgac agctatgaag tggatgactc
     1321 ccgcggacag agcaactgga cggccggctt cttcgacgcc ttccgcagcc accgtggcta
     1381 cgatctccgt gaacacctgc ccgccctctt cggccgagac acaaaagaga acaacggccg
     1441 cgtcctctgc gactaccgcg agaccatctc cgacctgctg ctcgaacagt tcacaaaacc
     1501 ctggcacgac tgggcacatg agcggcgcgc cctcatccgc aaccaggccc acggctcccc
     1561 ggccaatatc ctcgacctct atgccgccag cgatattccc gagaccgaag gccaggacat
     1621 cctgcgttat aaattcgcca cctccgccgc ccacgtcacc ggcaaacgcc tggcctcggc
     1681 agaaacggtc acctggctca acgaacattt cctcagcacc tggagcgacg tgaaaaaagc
     1741 cctcgacggc ttcttcctcg gcggggtcaa ccatgtcttc tatcacggca ccgcctatac
     1801 accggaaagc gacccctttc cgggctggct cttttatgcg tccgtccact ttaccccgaa
     1861 cgacccttcc tggccggact tcgccgcgct gaaccaatac gtcgcccgct gccagtcctt
     1921 cctgcaacag ggcgccccgg acaacgatat cctgctctat taccccatct atgacagctg
     1981 gtctgaatcc ggtgccggta acgcgttgtt gaaacactac gaccgcatgg acccggagtt
     2041 caccaacact ggcttcaaag aatgcgcgga gtacctgcaa tcccacggct acgcctttga
     2101 ttatgtctcc gacaaacagt tgcaggggcc gcttccggct caatacagga ccatcctcct
     2161 gcccgactgc cgctacctcc cgaccgacgc cctccagcgg ctgatcaacc tcgaaaaaca
     2221 aggcatcacc gtcctcgtct ataaagatct cccatccggc cctcccggtc tcggcgatct
     2281 tccacaacgc accacggcct tccggcaact gttggccaaa aatggctttg ccgtcggcgc
     2341 cgatctcaac accctgctca gcggagcccg tctccgccgc gaaaccctcg tcgacgacag
     2401 cctcggcttc attcgccgca cctatggtaa cggtaacgtc tacttcctcg tcaacaaagg
     2461 ctcaagatcc ttcaccggct gggcgccgct tgcggtgaaa ttcaccgccg ccggtatctt
     2521 cgaccccatc accggcgaga aaggcctggc gcaggtgaga gacaacgccg tctatctgca
     2581 actgcggccc ggcgagtcct gtctcgtcga gaccaatgtc gccccacgcg gcccgctgtt
     2641 tgcctactat gaacccgccg gcaacccgat cccgttgaag ggcgactggc agctcagctt
     2701 cctctccggc ggcccctccc tgccaaagcc cgtcacgctg acccaactgg cgccctggac
     2761 cgataaaacc ttctccggca cggccaaata ttcgatcagc ttcccccgtc cggcgggtgc
     2821 acccactgcc tggctcctcg atctcggcag ggtcgtccgc aacgcggaga taagcctcaa
     2881 cggccaatac
//
LOCUS       DATGFG010000054         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1475051, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000054 DATGFG010000000
VERSION     DATGFG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475051"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1474)
                     /locus_tag="VKQ52_01045"
     CDS             complement(89..1474)
                     /locus_tag="VKQ52_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ85786.1"
                     /translation="MKRMSRTYIIFLLAAGMVLGACKKGFLSTLPSSSIVEPTTLANF
                     QSILDNTTVMGRTPVLGELSADNFYLPYSAWQALDPREYNAYVWATDIYQGQGQVEDW
                     DAPYTQVYYANTVLEQLPTVPIVTENAQQWAEEQGSALFLRAYAFYNIAQLFAPAYDT
                     NSAASDLGIPLRLHSDVKAPSVRASVAATYTQILSDLRQASALLSPEISLSYRNRPSK
                     PAALAMLARVYLSMRDYTHAGLYADSALQLYDSLIDYNTVSAAGVRPFTKLNAETIYQ
                     SNFLSYTQCLEANTYPNCIVDSLLYQSYAANDLRRILFYTINANGHPNIKFGYSGLAF
                     PFSGLATDECFLIRAECAARAGNTTAALNDLNTLLKNRWKTGSFVPVTAASPAEALDI
                     ILAERRKELAFRGIRWSDLRRLNKGGKNITLQRVLNGITSTLPPNSPLYTLPIPPDVL
                     NDNPGMQQNQR"
     gene            complement(1480..4920)
                     /locus_tag="VKQ52_01050"
     CDS             complement(1480..4920)
                     /locus_tag="VKQ52_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ85787.1"
                     /translation="MNYLLKSIGAASLLMQGIISTAQSLHGVTLMEEHASLLKVLGDI
                     RRQTGYLYFVDSVSLALASPISFSVTNAPVSVAMDSCVRDQPLFYRLTGTSIDVRPGG
                     LVRGRVVDQQGHPIAGASVRGIDVKYPSGTSTDDQGSFRLRLAGQRFHLVVSCIGYGS
                     REYDLTGNAVLLVTLQGKAGDLNEVIVSDGFQDIPRDRAAGSFGKVKREDLELRVLPS
                     LVDRLDGVIGSLLVNKNILAGTNQSAFTIRGRSTIFSNPDPLIVIDNFPYNGDLSNLN
                     PEDIESVTLLKDAAAASIWGTRAANGVVVIKTRQGKYNEHTRLSFSSSLTVGQKPNVY
                     YTPILSPPDFIQVEQYLFGQGFYDGRITSLLHPALSPVVEILLQQRQGSISAADASTQ
                     LNVLRGQDTRRELDRYYYQPSINQQYWLGLHGGSATNRFFLSAGLDKDRSGLVRNAYD
                     RITVNGSNTYSLIPKKLELTSSVAFTSSTTYNNNPGGIAVNYPYLKLADAAGNALAVP
                     FQLRMGYVDTAGQGRLLDWHYRPLDELRNANNATRLTDYRLDFGLRYSFGKGWQAQAL
                     YHYGQGSSDDQNLQSLATFYTRNLLNEFTQVDAARQYSYPVPIGGILDEYLDTYHSQN
                     GRLQLNYDHRFAADHDLHVLAGSDVQDVESRVKTSRVYGYDPTSQSGLPVSSYTTPYT
                     QYSGAGTQALIPYPDNNISGADHYLSYYANGGYQYRQRYTVTASGRIDQSNLFGVNTN
                     RKTVPLWSAGVAWELSREGFYPSDSRLSLLRLRLTDGYNGNVYKAVGGYTTVYATPGI
                     SSTYLNSYGAPTANITNPPNPDLRWEKVQVINAGLDFGTRDSLLEGSLDYYSKNGQGL
                     IGPEALDPTSGNTQYIANAANMITHGLDLTLRVHTSFGPVRWTSTILFNFVRDRVTRY
                     LAKAGTVATYLNQYFFNPLAGRPLYSVYALRWAGLDPKTGDPMGWLNGHPSENYAAIT
                     GSADFTTLLYKGPANPPFFGSWRNSFAWKQWGLSLGLTYKFGDFFTRPSIQYAALFNG
                     SSGHPDYDRRWQQPGDERHTYVPSMIYPASIDRDNFYGYADVLIEKGDFIRLQDLQLF
                     YDLPGKDHPKKPRPAVRFFVFANNIALLWTANKQGIDPDALNSLPNPRSVAVGCKMKL
                     "
     gene            complement(4917..>5353)
                     /locus_tag="VKQ52_01055"
     CDS             complement(4917..>5353)
                     /locus_tag="VKQ52_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HLZ85788.1"
                     /translation="LGDGSVLAILGDICKMHDELRILVYSSEPASVYRDSLRQEFGIR
                     YVSKNESASGTVREMIEFVQNQRPRNHARSEGGNGVFDGLAPREKEILPYLIQDWNSA
                     QIGEKFGVDPATIRGLKKRILSKTNARNNEDLRNLARFHNRP"
BASE COUNT         1207 a         1357 c         1642 g         1147 t
ORIGIN      
        1 attacttcat gatcaccact taccccaccg ttaaaacacc ttttattcgt taagtcaacg
       61 acattagtta gttcactcaa ttcccttttc atcgttgatt ctgctgcatt cccggattat
      121 cattaagcac atcgggtgga ataggtaagg tataaagggg gctgttgggc ggcagggtag
      181 aagttattcc gttcaatact cgttgcaggg tgatattctt tcctccttta ttgagccgtc
      241 gcagatccga ccatcggatg ccccggaagg cgagctcctt ccgtcgttcc gcaaggatga
      301 tatcgagggc ttctgccggg gaagccgccg tcacaggtac aaagctgccc gtcttccacc
      361 ggttcttcaa cagggtattg aggtcgttga gtgcagccgt ggtattgccg gctctggcag
      421 cgcattcggc ccggatgagg aaacattcgt cggtggccag cccgctgaag gggaaggcca
      481 gcccactgta gccgaattta atgttgggat ggccatttgc attgatggta tagaaaagaa
      541 tgcgtcgcag gtcattggca gcataggact gataaagtag ggaatcgacg atacagttgg
      601 ggtaggtgtt tgcctccagg cattgggtat aggagaggaa attggactgg tagatcgtct
      661 cggcattgag cttggtaaag gggcggacgc cggctgcgga tacggtattg taatcgataa
      721 gggaatcgta gagttgcaag gcactgtccg catacagtcc ggcatgggta tagtcccgca
      781 tactgaggta cacccttgcg agcatggcca gcgcggcagg tttcgaaggg cggttacggt
      841 aactgagtga gatctccggg gagagtaagg cgcttgcctg gcggagatcg gaaagtatct
      901 gggtataggt agccgccacg gaagcccgga cagaaggagc ttttacatcg gagtgaagcc
      961 gaagcgggat gcccaggtcg cttgcagcgg aattggtgtc ataagccgga gcgaataact
     1021 gggcgatatt ataaaaggcg taggcgcgaa gaaaaagggc gctgccctgc tcttcggccc
     1081 actgctgggc attctcggta acgatgggga cggtgggcag ttgttcaagg acggtgttgg
     1141 cataatagac ctgcgtgtaa ggggcatccc agtcttccac ctgtccctgc ccctggtaga
     1201 tgtccgtagc ccagacatag gcattgtact cgcggggatc gagggcctgc caggcggaat
     1261 aggggagata gaaattgtcg gcggagagtt cgcccaacac aggcgttcgt cccatgacag
     1321 tggtattgtc gaggatggac tgaaagttgg ccagggtagt aggttcaaca atggacgaac
     1381 tgggaagggt gctgagaaaa cccttcttac aagccccgag gaccatcccg gcagccagca
     1441 aaaaaatgat ataggttcgg ctcattcgtt tcataacgtt tataatttca ttttacatcc
     1501 cactgccaca cttctgggat tgggcaaact gtttaaggca tcggggtcga tgccctgttt
     1561 attggcagtc cagagcaggg caatattatt ggcgaagaca aagaaacgga cggcgggcct
     1621 gggctttttc gggtggtcct ttccgggcag gtcatagaag agctggaggt cctgcaggcg
     1681 gatgaagtcg cccttttcga tcagcacatc ggcatagcca taaaaattat ctctgtcgat
     1741 actggcgggg tagatcatgg aaggcacata agtgtgccgc tcgtcgcccg gctgctgcca
     1801 ccggcggtcg taatcgggat ggccggagct gccattgaac aacgcggcat actggatgga
     1861 gggacgggtg aagaagtcgc cgaacttata ggtaaggcca agggaaagcc cccactgctt
     1921 ccaggcgaag ctgttacgcc agctgccaaa gaaaggcggg ttggccggac ccttatagag
     1981 gagggtggta aagtcggccg agccggtgat ggcggcatag ttctcactgg gatggccgtt
     2041 gagccagccc atggggtcgc cggtctttgg gtcgaggcct gcccaccgca gggcatagac
     2101 ggagtagagc ggccttccgg cgaggggatt aaaaaagtac tgattgagat aagtagcaac
     2161 agtgccggcc tttgccaggt agcgggtcac cctgtcgcga acaaaattga acaggatagt
     2221 acttgtccat cggacgggtc caaaggaggt atggacccga agcgtaaggt cgaggccatg
     2281 agtgatcata ttggcggcat tggcgatata ctgggtattg ccggaggtcg ggtccaaagc
     2341 ttccggtccg atcagcccct gcccgttctt gctataatag tcgaggctgc cttccagcag
     2401 gctatccctg gtgccaaagt cgagcccggc attgatgacc tgcacctttt cccatcgcag
     2461 atcagggtta gggggattag tgatgttagc tgtaggggcg ccataagagt taagataggt
     2521 agaactgatt cccggagtag cataaacggt agtataccct cccacagctt tatagacatt
     2581 cccgttgtat ccatcggtca atctcaaccg cagcagggag agccggctgt ccgatggata
     2641 gaagccctcc cgggagagtt cccaggcgac ccctgccgac cagaggggca cggtcttccg
     2701 gttggtattg acgccaaaga gattggactg gtcgatgcgt ccgctggcgg tcacggtata
     2761 acgctgccgg tactgatagc cgccattggc ataataggac aggtaatgat ctgcgccgga
     2821 gatattattg tcaggatagg ggatgagggc ctgtgttccg gcaccggagt actgggtgta
     2881 aggggtggta taactgctca cgggcaggcc gctctgcgag gtcggatcgt agccatacac
     2941 gcgggacgtt tttaccctgc tctccacatc ctgcacatcc gacccggcca ggacgtgcag
     3001 gtcatggtca gcggcaaagc gatggtcata gtttagctgc aaccggccat tttgagaatg
     3061 gtaggtgtcg aggtactcat cgaggatgcc gccgatgggg acagggtagg agtactgcct
     3121 ggcggcatcc acctgggtga actcattgag gaggttacgg gtatagaagg tggcgaggct
     3181 ttgcaggttt tgatcatcgg aagacccctg tccatagtgg tagagggcct gggcctgcca
     3241 ccccttccca aacgaatacc gcaggccgaa gtcgaggcgg taatcggtaa ggcgggtcgc
     3301 gttattggca ttgcgcagtt catcgagggg ccggtaatgc cagtcgagca gacgcccctg
     3361 gccggcagta tccacatacc ccatccgcaa ctgaaagggg acggccaggg cattgccggc
     3421 ggcatcggcc agcttcaggt agggataatt gacggcgata cccccgggat tattgttata
     3481 ggtggtcgag gaagtaaagg cgacactcga ggtcagttcc agctttttag ggataagcga
     3541 ataggtatta ctgccgttaa cggtgatgcg gtcataggca ttgcgcacca gcccgctcct
     3601 gtccttgtcg aggccggcgg agagaaagaa gcggttggtg gcgctgccgc cgtgcaggcc
     3661 gagccagtac tgttgattga tgctgggctg gtaatagtag cggtcgagct cgcgccgggt
     3721 atcctgtccg cgcaggacat tcaattgcgt gctggcatcg gccgcggaga tcgacccctg
     3781 ccgttgctgc aggaggatct ccacgacggg cgagagggcc ggatgcagga ggctggtgat
     3841 acggccgtca tagaacccct gtccgaacag gtactgctcc acctggatga aatcgggcgg
     3901 cgacaggatg ggggtataat agacattggg tttttgcccc acggtcaggc tggaggagaa
     3961 ggagaggcgg gtatgttcat tatacttccc ctgcctggtc ttgatgacga caacgccatt
     4021 ggcggcgcgg gtaccccaga tggaagcggc ggcggcatcc ttgagcaggg tcaccgactc
     4081 gatatcctcg gggttgagat tgctgaggtc gccattataa gggaaattat cgatgacgat
     4141 aagcgggtcg ggattgctga agatggtgct gcggccgcgg atggtgaagg cggactgatt
     4201 ggtgccggca aggatattct tattgaccag cagactgccg atgacgccat ccagccggtc
     4261 gacgagggaa ggcaggacac ggagctcgag gtcttcccgc ttcaccttgc caaaggaccc
     4321 ggcggccctg tccctgggaa tatcctgaaa gccatcggag acgatcacct cgttcagatc
     4381 accggccttg ccctggaggg tgacgagcaa gacggcattg ccggtgagat catactcgcg
     4441 ggagccatag ccgatacaac tgacgacaag atggaaacgc tgaccggcca gccgcagacg
     4501 gaagctgccc tggtcatcgg tagaggtgcc ggaaggatac ttcacatcta tccctctgac
     4561 ggaagcgccg gcaatgggat gcccctgctg atcgaccacc cttccccgga caagcccccc
     4621 gggccggaca tcgatggaag tgccggtcag ccggtagaag agcggctgat cccggacaca
     4681 actatccatt gccacactca ccggtgcatt ggtaacggaa aaactgatgg gggaggccag
     4741 ggccagcgac accgaatcga caaaatagag ataccccgtc tgccgccgga tatcccccaa
     4801 caccttcaac aaagaagcat gctcctccat aagcgttacg ccgtgcaaag actgcgcagt
     4861 ggaaattata ccctgcatca gcaggcttgc cgccccgatc gatttgagaa gatagttcat
     4921 ggtctgttat ggaaccttgc caggtttctt aagtcttcat tattcctcgc attggtttta
     4981 ctcaggatcc gcttttttaa cccccggata gtagccggat caaccccgaa tttttctcct
     5041 atctgcgccg aattccaatc ctggatcaga taagggagaa tctctttttc ccttggcgca
     5101 agcccatcga ataccccatt tcccccttcg gaacgtgcgt gatttctggg acgttggttc
     5161 tgcacgaatt cgatcatctc ccggaccgtc ccggacgccg attcattctt ggaaacataa
     5221 cgtatcccaa actcctgccg caacgaatcc ctgtagacgc ttgcaggttc gctggaatag
     5281 accagtatcc gtaattcatc atgcattttg cagatatcac caaggatcgc cagcacactg
     5341 ccgtcgccaa gcg
//
LOCUS       DATGFG010000055         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_789590, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000055 DATGFG010000000
VERSION     DATGFG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789590"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VKQ52_01060"
     CDS             <1..313
                     /locus_tag="VKQ52_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85789.1"
                     /translation="DNSAAIKHKWQVTSINGEALRYVGQPADYFDFGNTDTLIEFVGG
                     RYDTLYYQLHSDGHTLDIHSFNGMVAYPGLAVKTLTNTQLIITGGRLNPPINIVDSLR
                     R"
     gene            343..1272
                     /locus_tag="VKQ52_01065"
     CDS             343..1272
                     /locus_tag="VKQ52_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ85790.1"
                     /translation="MYFTQLPDHTQPGFDEQSHFGRFKKHNMIFNARGSQAHCDKHVG
                     CLSFKTVLGGEEWYGIDGHHIAVRPGQFLVLNDDQPYSCRIPKGEPADVLSIFFKKEF
                     ARAIFHDASHNEETLLDDPANPGNNPVEFFQTLYSIEPELQTQLTAFIIHLETQGYDS
                     DAADEHLIFILRYLILTHRSQQTHTIRVDAQKPSTRLEIFKRLCIAKDLLHSSYKQPL
                     DLQTLSRNACLSVPQLIRQFRSVFHTTPHRYLVKLRLEHAAHLLTTTTLPVREITWQC
                     GFENPSAFCRAFRSAYNTQPQNFRDHASHTPIS"
     gene            1279..1551
                     /locus_tag="VKQ52_01070"
     CDS             1279..1551
                     /locus_tag="VKQ52_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HLZ85791.1"
                     /translation="MPTIHLLIKGKVQGVCYRASAKDQADRLSLTGWVRNTREGHVEI
                     TATGEPTALQQFTTWCRQGPDHAVVTSVETQNLPEKSFPSFNILRG"
     gene            1720..>3224
                     /locus_tag="VKQ52_01075"
     CDS             1720..>3224
                     /locus_tag="VKQ52_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ85792.1"
                     /translation="MSQLLAQNRTITGKITDDKGTPIPHASIFVKSNKPLFGTTAAQD
                     GSYSVSVPPTARTIVVSSVNFQPLELSIGDRGIINFTLTPVSSSLDEVVVTGYGTQKK
                     SANTASIVKVGGEKLEDRPFTSVDQMLQGASAGLQSSATTGQPGANQAIRIRGVGSFS
                     YGGAQPLYVIDGVQINGGDLANGNGTTTTGAGSFNINPSTNVLATLNSNDIESISILK
                     DAAATAIYGSRGANGVIVITTKSGKLGKAQFRFDAEVGQNSPILPPKNGLPLRKKDWI
                     NMLNEGMVNGGYPAATITSTLHSYGDTSNIDTDWMGLVTRKALQQQYNLSVSGGEGKL
                     KYFLSGGYFKQQGTTIATDLQRVTGDLKITYTATDRLTLTTKITAGDVIQNSALASSG
                     TSGGGGYFGNPNYVATTLRPTQNPFNADGTLNISTSNNYGFPSHYNPLFIAANDKRWL
                     KAFQGLANQSLNYKILEGLKYTSTMGLQYATDEEYQYNNPFHGDASGSSGEG"
BASE COUNT          830 a         1172 c          653 g          569 t
ORIGIN      
        1 agacaatagc gccgccataa aacataaatg gcaggtcacc tccatcaacg gagaagcgct
       61 cagatacgtc gggcaaccgg cggactattt tgacttcggc aacaccgaca cgctcatcga
      121 attcgtcggt ggccgttacg acaccctcta ctaccagctc catagcgacg gacacacact
      181 cgatatccac tcctttaacg ggatggtcgc ctatcccggc ctcgcggtaa aaacgctcac
      241 aaacacccaa ctgatcatca ccggcggccg attaaaccca cccatcaaca tcgtggactc
      301 cctccgccgc tgattgtaaa aagaattacc gagattagtg gtatgtattt cacccaacta
      361 cccgaccaca cccaaccagg tttcgacgaa cagtcgcatt tcggccggtt caagaaacat
      421 aatatgattt tcaacgcccg cggcagccag gcccattgcg acaaacacgt cggctgcctg
      481 tcttttaaga ccgtcctcgg cggcgaggaa tggtacggca tcgacggcca ccacatcgcc
      541 gtccggccgg gacagttcct tgtcctcaac gacgaccagc cctactcctg ccgcatccca
      601 aagggcgaac cggcagacgt cttatccatc ttctttaaaa aggaatttgc gcgcgccatc
      661 ttccacgacg ccagccataa cgaagaaacc ctgctcgacg atccggcaaa ccccggcaac
      721 aacccggtcg aattcttcca aaccctctat tcgatcgaac ccgaacttca aacccaactc
      781 actgccttta tcatccacct cgaaacccag ggatatgata gtgatgccgc ggacgagcat
      841 ctcatcttca tccttcgcta cctcatcctc acccaccggt cccaacaaac ccacaccatc
      901 agggtcgacg cacaaaaacc ctccacccgc ctcgagatct tcaaacgact ctgcatcgcc
      961 aaagacctcc tccattcctc ctataaacaa cctctcgatc tccaaaccct aagcaggaat
     1021 gcctgcctct ccgtccccca actgatccgt caattccgct ccgtcttcca cactactccc
     1081 caccgctacc tcgtaaaact ccggctggaa cacgccgcgc acctcctcac caccaccacc
     1141 ctccccgtcc gggagatcac ctggcaatgc ggcttcgaaa accccagcgc cttctgccgc
     1201 gccttcagat ccgcctacaa cacccaaccg caaaatttca gagaccacgc ctcccacact
     1261 ccaatatcct gatcatccat gccaaccatc cacctcctca taaaaggcaa agtccagggc
     1321 gtctgctacc gcgcctccgc aaaagatcag gccgaccgcc tctccctcac cggctgggtt
     1381 aggaacaccc gcgaaggcca cgtcgagatc accgccaccg gtgaacccac cgccctccaa
     1441 caattcacca cctggtgccg ccagggcccc gaccacgccg tcgtcaccag cgtcgaaacc
     1501 caaaacctcc ccgaaaaaag cttcccctcc ttcaacatcc tccgcggctg attcccggca
     1561 ttctacaaat atatttttta gaaaaatatg ttaggcggta gctaccgcgg tagtttttaa
     1621 atttattttt aacgctgtat tagatcttat ttaatcaaac gctaactgct acacatgaac
     1681 aaacacctac tgcttgtaat ggcatgcata tgcatgtctg tgagtcagtt attggctcag
     1741 aacaggacca taaccggcaa gatcactgac gacaaaggaa cacccattcc ccacgcatcc
     1801 atcttcgtaa agagcaacaa gcctctcttc ggcaccaccg ccgcacaaga tggttcctac
     1861 tcagtatctg ttccgcctac agcaaggacc atcgtggtct cctccgtcaa cttccagccc
     1921 ctggaactct ccatcggcga cagaggcatc atcaatttca ccctcacacc cgttagttcc
     1981 agcctcgacg aagtagtcgt aacgggatac ggtacccaga aaaaatcggc caacaccgcc
     2041 tccatcgtca aagtaggcgg cgaaaaactc gaagaccgcc ccttcacctc cgtcgaccag
     2101 atgctgcagg gtgcctcggc cggtctccaa tcctcggcta caaccggcca gcccggcgcc
     2161 aaccaggcca tccgcatccg gggtgtcggc agttttagct acggcggcgc ccaacccctc
     2221 tacgtcatcg acggagtcca gatcaacggc ggcgacctcg ccaacggcaa cggcaccacc
     2281 accaccggag caggaagctt taacatcaac cccagcacca acgtcctggc caccctcaac
     2341 tccaacgaca tcgaaagcat cagcatcctc aaggacgcag ccgccaccgc catctacggc
     2401 tcccgtggcg ccaacggcgt catcgtgatc acgacaaaaa gcggcaaact cggtaaggcc
     2461 cagttccggt tcgacgccga agtcggtcag aacagcccca tcctgccccc caagaacggc
     2521 ctgccgctgc gcaaaaaaga ctggatcaac atgctcaacg aagggatggt caatggcggc
     2581 tacccggcgg caaccatcac ctcgaccctc cactcctacg gcgatacttc caatatcgac
     2641 accgactgga tgggcctggt cacccgcaaa gccctgcaac aacaatacaa cctcagcgtt
     2701 tcaggcggcg aaggcaaact gaaatacttc ctctccggcg gctactttaa acaacagggc
     2761 accaccatcg ccaccgatct ccaacgggta accggcgacc tgaagatcac ctacacggcc
     2821 accgaccgcc tgaccctgac caccaagatc acggccggcg acgtcatcca gaactctgcc
     2881 ctcgcatcca gcggtacatc gggcggcggc ggttatttcg gtaaccccaa ctatgtagcc
     2941 accaccctac ggccaacaca gaacccgttc aacgccgatg gaaccctgaa catcagcacg
     3001 agcaacaact acggcttccc ctcgcactac aacccgctgt tcatcgccgc caacgacaaa
     3061 agatggctca aagccttcca gggcctcgcc aaccaatccc tgaactataa gatcctcgaa
     3121 gggctcaagt acaccagcac catgggcctc caatacgcca cggacgagga gtaccagtac
     3181 aacaacccct tccacggcga cgcatccggt tcttccggcg aagg
//
LOCUS       DATGFG010000056         5796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_448364, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000056 DATGFG010000000
VERSION     DATGFG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448364"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VKQ52_01080"
     CDS             <1..250
                     /locus_tag="VKQ52_01080"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85793.1"
                     /translation="VDVSKATGNRLSLEWLLRAYALFPDKEHFFSGNGSHFDRLAGSS
                     LLRQQLRDGLTEEQIRGSWEPALGNFKTIRKKYLLYAE"
     gene            240..1181
                     /gene="fmt"
                     /locus_tag="VKQ52_01085"
     CDS             240..1181
                     /gene="fmt"
                     /locus_tag="VKQ52_01085"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HLZ85794.1"
                     /translation="MPSEKREPRIVFMGTPEFAVASLDAILEAGFSVVAVVTAPDKPA
                     GRGMQLTESAVKKYAREKGLPILQPEKLRDPGFLERLRELRADLQIVVAFRMLPEVVW
                     NMPPMGTVNLHGSLLPQYRGAAPINWAVINGEKETGVTTFQLLHDIDTGSILLQDRFP
                     IADTDTAGDVHDRMMVIGARLLVRTIEGLAAGSLRSTPQENPAGLKHAPKIFAETGRI
                     DWTKPTVEVYNLIRGLSPFPAAHTSLDGKMLKIYRAEKDIHVLAPDVAPGVYDTDGKT
                     YLRFATADGYIRVTEIQLEGKKRMPVGDFLRGYRFNS"
     gene            complement(1171..2292)
                     /locus_tag="VKQ52_01090"
     CDS             complement(1171..2292)
                     /locus_tag="VKQ52_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLZ85795.1"
                     /translation="MRSQVTVLFLISFFFIKFAKSQTSNELTVYGQTGKLYLEHTVVP
                     KENWYSVGRLYNVSPKELAPFNHLALTQPLVIGQTLQIPLTAVNFSQNGQKTPAETLI
                     PVYHITQEKEWMYRISVNHNKVPIPTLEKWNNMNKDAVRSGMHLIVGYLKVKTALSAL
                     ATGGTSSPVAATARPATTPPPATTTPPAATPPAAATTTPPTTTPPATATPPATTAQTA
                     SATQTPTTAAAPTRSLAVKTTDSPIAKPASAPPTYKAPATTPGNPPANTPPPPHFDGG
                     VFKSEFLDNGKTISGTAGIFKSTSGWQDGKYYALMNNVAIGSIVKVTDAAGGKIVFVK
                     VLGQLPDMKESVGLTIRISNAAAAEMGQTGDRFSVQVSY"
     gene            complement(2335..3699)
                     /locus_tag="VKQ52_01095"
     CDS             complement(2335..3699)
                     /locus_tag="VKQ52_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85796.1"
                     /translation="MSSPLPKELLSSLEGLPGYDAAAFEAVHAAGAAPTSIRINPAKP
                     PVNLPANTTPVPWSSFGYYLETRPVFTFDPLFHAGTYYVQEASSMFLEQALRQTVDLS
                     RPLRVLDLCAAPGGKSTLLQSLLSPESLLVSNEVIRNRVHILQENMIKWGSANTVVTS
                     NDPRDFQRLENYFDVIVVDAPCSGSGLFRREPDAIDEWSPDNVQLCWQRQQRILADCW
                     PALRRDGILVYSTCSYSPEEDEHLLDWITTELDGAGCRLQTDPEWNIVETTGKLGAYG
                     YRFYPHRLKGEGFFIACVRKNEGSVFAPPRKSSHPGRPAKKEQEALHRWVHSETPLAF
                     FTHQEQIHAFPESRLADLSLLQNACYLKEAGLSLGQLSAKDFIPHHQLAMSTIVHPAV
                     TALPLTREQALQYLRKEELHPETTHRGWALVQYEHRNLGWIKVLPQRSNNYYPKEWRI
                     LKRE"
     gene            complement(3775..4569)
                     /locus_tag="VKQ52_01100"
     CDS             complement(3775..4569)
                     /locus_tag="VKQ52_01100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ85797.1"
                     /translation="MYSSLRLAQKYFRYWLTAANGKGHGIHSPFVFDFVTKVLNDKKP
                     YPAYDPIEGLRRRLLGDNTLLEVEDQGAGSAYAATRTRSIADIARRAAKPSRLGQLLH
                     RVALRYQPSTIIELGASLGLSAAYLASGAPAATLYTIEGAPAIAAAAERNLRSLHLPA
                     TILPGAFDDVLPGLLPTLPPVDLAFVDGNHRLDPTLRYFDLLLRHSANSSVLIFDDIH
                     WSAEMETAWATIKADPRVYLTIDLFFIGFVFRRDEFRVKQDFVIRF"
     gene            complement(4575..5078)
                     /locus_tag="VKQ52_01105"
     CDS             complement(4575..5078)
                     /locus_tag="VKQ52_01105"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HLZ85798.1"
                     /translation="MIISLIVAASTNNAIGKDNKLLWHLPNDMKYFRNTTWGMPVIMG
                     RKTYEALAGEPLPGRFNFVVTRQKDWQPASEKATVTSTLDEALRGAADTDCKEAFVIG
                     GGEIYALAMPIADKIYMTRVHAVLEGDTWFPPIDEKEWELTDNRDFPADDKHAYAYSF
                     QVWTRKK"
     gene            complement(5125..>5796)
                     /locus_tag="VKQ52_01110"
     CDS             complement(5125..>5796)
                     /locus_tag="VKQ52_01110"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF002497.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HLZ85799.1"
                     /translation="TKKLHLKSIIHELIWFLRGDTNIAYLKEHGVSIWDEWADADGEL
                     GPVYGKQWRSWEGANGKTVDQITQLIDQIKKNPDSRRLIVSAWNVADLPEMALMPCHA
                     LFQFYVAPDAEGKQRLSCQLYQRSADVFLGVPFNIASYALLTLMVAQVCGLEPGEFIH
                     TFGDVHLYSNHLEQARLQLTRSPYPLPTMKLDPAVKDIFQFRFEDFTLENYQSHPAIK
                     APVAV"
BASE COUNT         1124 a         1448 c         1929 g         1295 t
ORIGIN      
        1 ggtggatgtg agcaaggcga cgggcaaccg gttgtcgttg gaatggctgc taagggccta
       61 tgcgctgttc ccggacaagg aacatttttt tagcggcaac ggcagtcact tcgaccggct
      121 ggcgggttcc tccctattgc ggcagcagtt acgggatggg ctaaccgaag agcagatccg
      181 tggcagctgg gagccggcgc tcggtaattt taaaaccatt cgaaagaaat acctgttgta
      241 tgccgagtga aaagcgcgaa ccaaggatcg tttttatggg cacgcccgaa tttgccgtag
      301 ccagcctgga tgccatcctg gaggccggtt tctccgtggt ggccgtcgtg acggcgcccg
      361 ataaaccggc gggccgcggg atgcaactga cggaaagcgc cgtaaagaaa tatgcccgtg
      421 aaaaggggtt gccgatcctg cagccggaga aattgcggga cccggggttt ttggagcggt
      481 tgagggagct tcgcgccgac ctgcagatcg tcgtggcctt tcgcatgctg ccggaagtcg
      541 tatggaacat gccgccgatg gggaccgtca acctgcatgg gtcgttgtta ccgcagtacc
      601 gcggggcggc ccccatcaac tgggcggtga tcaacgggga aaaggagacc ggtgttacta
      661 cctttcaatt gctacacgat atcgacacgg ggagtatcct gttacaggat cgttttccca
      721 tcgcggatac cgatacggcg ggcgatgtac acgatcggat gatggttatc ggcgcccggc
      781 tgctcgtgcg cacgatcgag ggtctggcgg cgggtagtct gcgatcgacg ccacaggaga
      841 acccggcggg gttgaagcat gcgccgaaga tctttgccga aacggggcgc atcgactgga
      901 cgaagcctac cgtggaagta tataacctga tcaggggact atcgcccttc ccggctgccc
      961 atacgagcct ggacgggaaa atgctgaaga tctatcgcgc cgaaaaagac attcatgtgc
     1021 ttgcgccgga tgttgcaccg ggagtgtatg acaccgatgg gaaaacctat ctccggtttg
     1081 ccacggcgga tggctatatc cgggtgaccg agatccagct ggaaggcaag aagcggatgc
     1141 ctgtcgggga ttttctgcgc ggctatcggt ttaatagctg acctgtactg agaaccggtc
     1201 gcccgtctgc cccatctccg ctgccgctgc gttgctgatc cggatggtaa ggcctacgct
     1261 ctccttcata tcgggcagtt gtcccagtac ttttacaaaa acgattttac cgccggcggc
     1321 atccgtcact tttacgatgc tgccgatggc cacattgttc atgagggcat agtatttacc
     1381 gtcctgccag ccgctggtgc ttttgaagat gccggcggtg ccgctgatgg ttttaccgtt
     1441 gtcgagaaat tccgatttga atacgccgcc gtcgaagtgc ggaggtggcg gggtatttgc
     1501 cggcgggttg ccgggagttg tagcgggtgc tttgtatgta ggcggcgcgg atgccggttt
     1561 tgcgatgggt gaatcggtgg tttttaccgc gagggatctt gttggcgccg ctgcggtggt
     1621 gggggtttgc gtcgccgatg ccgtttgcgc cgtcgtggcc ggtggcgtcg cggtagccgg
     1681 tggtgtggtg gtgggtggtg tggtcgtggc ggcggcgggc ggcgtggcgg ctggcggtgt
     1741 tgttgttgcg ggcggcgggg tcgttgcggg gcgagcagtg gcggctacgg gagacgaggt
     1801 tccgccggtg gccagcgcgg acagggccgt tttgaccttc aggtaaccca cgatgagatg
     1861 cattccggag cggaccgcat ccttattcat attgttccac ttttccagtg tggggatggg
     1921 caccttgtta tggtttacac tgatgcgata catccattct ttttcctggg tgatatggta
     1981 tacggggatc agggtctcgg cgggggtttt ctggccgttc tgggaaaagt tcacggccgt
     2041 cagcgggatc tgcagggtct ggccgatcac caggggttgg gtcagcgcca gatgattgaa
     2101 gggggccagt tctttggggc tgacattgta cagccggccg acgctatacc agttctcctt
     2161 tggcaccacg gtgtgttcca ggtacaattt acctgtctgg ccgtagacgg tcaattcatt
     2221 ggatgtttgg gatttggcaa actttataaa aaaaaagctg atcagaaaaa gtacggtcac
     2281 ttgtgatcgc atagcattat ttttttatga cgttcgaagc aaagatgcgg ttttttattc
     2341 tcttttgagt atccgccatt ccttcggata gtaattgttc gagcgctggg gcaggacctt
     2401 gatccagccc aggttgcgat gctcatattg cacgagcgcc caaccgcggt gtgttgtctc
     2461 cggatgcagc tcctccttcc ggaggtattg cagcgcctgt tcacgcgtga ggggcagggc
     2521 ggtgaccgcg ggatgcacga tcgtgctcat cgccaactgg tggtggggga taaagtcctt
     2581 tgcggacagt tgccccaggg aaagtcccgc ctctttcagg tagcaggcgt tttgcaggag
     2641 ggacagatcg gccagccggg attccgggaa ggcatggatc tgctcctggt gggtaaagaa
     2701 tgcaagcggg gtttccgaat gcacccagcg gtgcagcgct tcctgttcct tcttagccgg
     2761 ccgcccggga tggctgctct tgcggggcgg ggcaaagacg cttccttcat tcttgcgtac
     2821 acaggcgatg aaaaagcctt cgcctttcag gcggtgagga tagaagcgat agccatacgc
     2881 cccaagttta cccgttgtct ccacgatatt ccattccgga tctgtttgca aacggcagcc
     2941 ggcgccatcc aattcggtgg tgatccagtc cagcagatgt tcgtcctctt cgggggaata
     3001 cgaacaggtg gaatagacca ggatgccgtc ccggcggagg gccggccagc aatcggccag
     3061 gatacgctgc tgccgctgcc agcagagttg cacgttatcc gggctccatt catcgatggc
     3121 atcgggctca cggcggaaga gaccgctccc gctgcagggc gcatcgacga cgatgacatc
     3181 gaaataattc tccagccgct ggaagtcacg ggggtcgttg ctggtgacga cggtattggc
     3241 gctcccccac ttgatcatgt tctcctgcag gatatgtacg cgattccgga tgacttcgtt
     3301 gcttacgagc aggctctccg gtgataaaag ggattgcaac agggtcgatt tgccgcccgg
     3361 cgccgcgcaa aggtcgagga cgcgcagggg acggctgaga tcgacggtct ggcgcagcgc
     3421 ctgttccagg aacatgctcg aggcttcctg gacataatac gttccggcat ggaagagcgg
     3481 gtcgaaggtg aagacgggac gggtttccag ataatagcca aaagaagacc agggcaccgg
     3541 tgttgtgttg gcgggcaggt ttaccggtgg tttggcgggg ttgatgcgga tggaggtggg
     3601 tgcggcgccc gcggcgtgca cggcttcgaa ggcggcggcg tcatagccgg gcaggccttc
     3661 gagggaagag agaagttctt ttggtagcgg agaggacaat cgatgcaaaa atattataaa
     3721 tcggcggttt ggtcaagaaa ggcgcggcgt agcacgcgga taaaaaaagt gttgtcagaa
     3781 ccggataaca aaatcctgct ttaccctgaa ttcgtcccgc cggaaaacaa agccgataaa
     3841 aaagagatcg atcgtcaggt atacgcgggg gtcagccttt atcgttgccc aggccgtttc
     3901 catttcggcg ctccagtgga tatcatcgaa aatgagaacg gagctgtttg ccgaatggcg
     3961 gagaaggagg tcgaaatagc gtaatgtggg gtccagccgg tgattgccat ccacgaaggc
     4021 gaggtcgaca ggcggcagcg tagggagtaa accgggcaac acgtcatcaa atgcgccggg
     4081 gaggatggtt gcgggaaggt ggagggatcg caggttgcgc tctgccgcgg cggcgatcgc
     4141 gggggcgcct tcgatggtgt acagggtcgc ggccggggcg ccggaagcca ggtaggcggc
     4201 agagagtccc agcgaagcgc cgagctcgat gatcgtggaa ggctgatagc gcagggcgac
     4261 gcgatggagc agttgcccca gccgggatgg ttttgccgcg cggcgcgcga tatcggcgat
     4321 gctccgggtc cgggtggctg cgtaggcgga gcccgcaccc tggtcttcca cttccaggag
     4381 ggtgttgtcg ccgagcaggc ggcggcggag tccttcgatg ggatcgtagg cgggatacgg
     4441 ctttttatcg ttcaggacct ttgttacgaa atcaaagaca aagggagagt ggattccgtg
     4501 ccccttcccg ttggccgcgg tgagccagta ccggaaatat ttttgtgcca gccggaggga
     4561 cgaatacatg ggcattattt tttccgcgtc catacctgga agctataggc ataggcatgt
     4621 ttgtcatcgg cgggaaaatc ccggttgtcg gtgagctccc attccttttc atcgatgggg
     4681 ggaaaccagg tatccccttc cagcacggcg tgtacccttg tcatatagat cttgtccgcg
     4741 atgggcatgg ccagtgcata gatctcgccg ccgccgatga caaaggcttc tttgcagtcg
     4801 gtgtccgcgg cgccccggag agcttcgtcc agggtggaag tgacggtagc cttttcagat
     4861 gcgggttgcc aatccttctg ccttgtgacc acgaagttga agcggccggg caagggttcg
     4921 cctgccaggg cctcataggt cttgcggccc atgatgaccg gcatccccca ggtggtattc
     4981 ctaaaatatt tcatgtcgtt cgggagatgc cagaggagtt tattatcctt accgatggca
     5041 ttattcgtag atgcggcgac gatgagggag atgatcatag ttttagctaa tgatgggcct
     5101 tcggccggcg agcctgtggc tcgcctagac agctaccggg gcttttatgg cgggatggga
     5161 ttggtaattt tcaagggtga agtcctcgaa ccggaactgg aagatatcct tcaccgcggg
     5221 atcgagtttc atcgtgggca agggataagg gctgcgggtg agttgcagcc ttgcctgttc
     5281 gagatgattg ctatacaggt gcacatcgcc aaaggtgtgg atgaattctc cgggctcgag
     5341 tccgcagacc tgggcgacca tcagggtcag cagggcatag gacgcgatgt tgaagggtac
     5401 cccgaggaag acatcggcgc tgcgttgata gagctggcaa ctgagccgtt gttttccttc
     5461 ggcgtcaggg gcgacataga actggaagag cgcatggcag ggcatgaggg ccatttcggg
     5521 cagatcggcg acattccagg cgctgacgat caggcggcgg ctgtcgggat tcttttttat
     5581 ctggtcgatg agctgggtga tctggtccac ggtctttccg ttagcgcctt cccagctgcg
     5641 ccattgcttg ccataaacgg gacccagttc tccgtcggcg tcggcccact cgtcccagat
     5701 gctgacgccg tgttccttca agtaagcgat gttggtatcg ccgcgcagga accagatcag
     5761 ctcgtggatg atcgacttga gatggagctt cttggt
//
LOCUS       DATGFG010000057         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1128328, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000057 DATGFG010000000
VERSION     DATGFG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128328"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..2681
                     /locus_tag="VKQ52_01115"
     CDS             321..2681
                     /locus_tag="VKQ52_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ85800.1"
                     /translation="MLHNYLKIAIRNLLRNKSFSAINIGGLAIGMASALLILLWVHNE
                     LSFDRWYPNTGRLYKLYNRDYFQGQLQAWPNTPKPLAPVVKKDYPAVEDVTRFRNGVF
                     LLTVGDTRLNSQGAYADSGFLAMFGFPLLKGDAAHALNSPKSIVLTQHLARTLFGNED
                     PMGRTVRVDSNNHFTVAAVLRDLPANTSFSFDYLLPWSFMMKLNTDNDQWGTNSVFTF
                     VLLKPGASQTAFDTQIKNITIDHSGHKETTQVFAQPMSRLHLYSKNENGVLVGDKIRI
                     VQLFALIAGFILLIACINFMNLYTARSEKRAREVGIRKVVGAVRGYLIGQFIGESIFI
                     AALSFALALLITHLTLDSFNQLVGKQLIIEFDNPLWWTFAAGFVVFTGLLAGSYPAFY
                     LSGFQPIKVLKGSFRKINALISTRKVLVVLQFTIAIVLIISTLIVRRQIQYAQDRDTG
                     YNRSNLIYTFSQGDIPKYYEAIKNDLLASGAVLSVNKSFNPITQHWSDSWGYSWDGST
                     PADKRVDFIHLSSDAGFVRTMGATLVAGRDIDVYQYPTDSTAVMLNEAAVRAMHVKDP
                     VGMTIRSDEQAHHVVGVLKDFVLESPYSAVAPMMIEGPTSFFQVIHMKLNPAWPAAAN
                     MAQVEKVFKRYNPQYPTPFIFVDDNYASKFKDEQQIGTMAALFAGLTIFISCLGLFAL
                     ATYMAENRVREIGVRKVLGASVTSITAMLAKDFVWLVLIAFLIAAPVAWLVMHKWLIG
                     YNYHSAIGWDIFALSGFLTLAIAVATVSYQAIRAAIANPVKALRSE"
     gene            complement(2698..>3462)
                     /locus_tag="VKQ52_01120"
     CDS             complement(2698..>3462)
                     /locus_tag="VKQ52_01120"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLZ85801.1"
                     /translation="EQVAEHVHLSPFHFQRMFREWAGVTPKQFLQYLTLGHAKQVLES
                     GATLSDAAFEAGLSGTGRLHDLFIKIEGMTPGEYKNGGEELPINYRFAESPFGPLLIA
                     STHKGVCYMAFADEGKAAALELLKEQFPNAQYHAARDAWQEEALAIFAQDWSQPREIK
                     LHLKGTPFQIKVWETLLKVPMGGLTTYAEVADSIQHPQGVRAVGSAVGANPVAFLIPC
                     HRVIRSNGQTGEYHWGGTRKQAILGWEAAHLETRVA"
BASE COUNT          720 a         1229 c          810 g          703 t
ORIGIN      
        1 ctggtggtgc atgcgccaat ggctcaacgg catcccgtat cacatcgaaa tgaactggtg
       61 gatcttcgcc ctcaccggtc tcctctccat cgtcatcgcc atcggcacgg ttggctacca
      121 ggcggtgcgt gcagccctta ccaatcccat cctcgtcctc cgcaccgatt gacccgccgc
      181 atcgctcatc ccggtccccc gcggcagcca attcgcacat cggctgtatc aaacccgaac
      241 acccgtcctc cagcaacaca aacaacaaat tgtcccccaa tgccctggca ttctggcacc
      301 ctcttcgctg cggtttcttt atgctacata actacctcaa aattgccatc cggaacctcc
      361 ttcgcaataa aagcttctcc gctatcaaca tcggcggact cgccatcggc atggccagcg
      421 ccctgctcat cctgctctgg gtccacaacg aactcagctt cgaccgctgg tatcccaata
      481 ccggccggct ctacaaactc tacaaccgcg attacttcca gggtcagctc caggcctggc
      541 ccaatacccc aaaacctctc gcccccgtcg tcaaaaaaga ctacccggcc gtcgaagacg
      601 ttacccgctt ccgcaatggc gtcttcctcc tcacggtcgg tgacacccgc ctcaactccc
      661 agggcgccta cgccgacagc gggttcctgg ccatgttcgg cttcccgctg ctgaaaggtg
      721 acgccgccca cgccctcaac agcccaaaaa gtatcgtcct cacccagcat cttgccagga
      781 ccctcttcgg caacgaagac cccatgggca ggaccgtccg ggtcgacagc aataaccatt
      841 tcaccgtcgc cgccgtcctc agggacctcc ccgccaacac ctctttcagc tttgactatc
      901 tccttccctg gtccttcatg atgaagctca acaccgacaa cgaccaatgg ggcaccaatt
      961 ccgtcttcac cttcgtattg ctgaaacccg gcgcttcaca gaccgcattc gacacgcaga
     1021 taaagaacat caccatcgac cactccgggc ataaagagac cacccaggtc ttcgcccagc
     1081 cgatgagccg cctgcacctg tactcgaaga acgagaatgg cgtccttgtc ggcgacaaga
     1141 tccgcatcgt ccagctcttt gccctcatcg ccggctttat cctgctcatt gcctgcatca
     1201 acttcatgaa tctgtacacg gcccgcagcg agaaacgcgc acgcgaagta ggtattcgca
     1261 aggtagtcgg cgccgttcgc ggttatctca tcgggcagtt catcggcgaa agcatcttca
     1321 ttgccgccct ctcctttgcc ctcgccctgc tcattaccca cctcaccctc gacagcttta
     1381 accagctcgt gggcaaacaa ctcatcatcg aattcgacaa cccgctctgg tggacctttg
     1441 ccgccggctt cgtcgtcttc accgggctgc tggccggcag ctacccggcc ttctatcttt
     1501 ccggcttcca acccatcaaa gtactgaaag ggtccttcag gaagatcaac gccctcatct
     1561 ccacccggaa ggtcctggtc gttctgcaat tcaccatcgc catcgtcctg atcatctcca
     1621 ccctgatcgt gagaagacag atccagtatg cgcaggatcg cgacaccggc tataaccgga
     1681 gtaacctgat ctataccttc agccaggggg atatcccgaa atattatgaa gcgataaaaa
     1741 acgacctcct tgcaagcggc gccgtactga gcgtaaacaa atcatttaac cccatcaccc
     1801 agcactggtc cgatagctgg ggctactcct gggacggcag cacgccggca gacaaacgcg
     1861 tcgacttcat tcacctgagc agtgatgccg gcttcgtccg gaccatgggc gcaaccctcg
     1921 tcgccggccg ggacatcgat gtctaccaat atccgacgga ctccaccgcc gtaatgctga
     1981 atgaggctgc cgtccgggcc atgcacgtaa aggaccccgt cggcatgacc attcgttcgg
     2041 atgaacaggc ccaccatgtc gtcggggtct taaaggattt cgtcctcgaa tccccctact
     2101 ccgcagtcgc cccgatgatg atcgaagggc cgaccagctt cttccaggtg atccatatga
     2161 aactgaatcc tgcctggccg gccgccgcca acatggccca ggtggaaaag gtcttcaaac
     2221 ggtataaccc gcagtacccc acgcctttca tcttcgtcga tgacaattac gccagtaaat
     2281 tcaaggatga acaacagata ggaaccatgg cggccctctt tgccgggctc accatcttta
     2341 tatcctgcct gggcctcttt gccctggcga cctatatggc cgagaaccgc gtccgggaga
     2401 tcggggtccg caaagttctc ggcgccagcg tcaccagcat caccgcgatg ctggcaaaag
     2461 attttgtgtg gctggtgctc atcgccttcc tgatcgccgc acccgttgcc tggctggtca
     2521 tgcacaaatg gctcatcggc tataactatc acagcgctat cggttgggac atcttcgccc
     2581 tctccggttt tctcaccctg gccatcgccg tcgccaccgt cagctaccag gcgatacggg
     2641 cagccatcgc caacccggta aaggcgctgc gcagcgaata atgggctttt gcttagccta
     2701 cgccacccgc gtttccaggt gcgccgcctc ccagccaagg atggcctgct tccgcgtacc
     2761 accccagtgg tactccccgg tctgtccatt gctccggatc acccggtgac agggaatcag
     2821 gaaagccacg ggattcgcgc ccaccgccga cccaacggcc cgcaccccct gcggatgctg
     2881 aatgctgtcg gccacctccg cataggtggt caacccaccc atcggaactt ttagcagggt
     2941 ctcccaaacc tttatctgga acggcgtccc cttcagatgc aacttgatct cccgcggctg
     3001 gctccaatcc tgcgcaaaga tcgccagcgc ctcctcctgc catgcatcgc gggcggcatg
     3061 gtactgtgca ttggggaact gttccttgag caactccagc gcggccgcct taccctcgtc
     3121 ggcaaacgcc atataacaaa cgcccttgtg cgtcgaggcg atcagcaacg gcccgaatgg
     3181 actctccgca aaccggtaat tgatcggcaa ctcttccccg ccgttcttgt attccccggg
     3241 cgtcatccct tctatcttga taaacaggtc atgcagccgc ccggtgcccg aaagccccgc
     3301 ctcgaaggcc gcgtcggaaa gcgtcgcccc cgactccagc acctgcttgg catgccccag
     3361 cgtcaggtac tgcaaaaact gcttcggcgt caccccggcc cactccctga acatccgctg
     3421 gaaatgaaag gggctcagat ggacatgctc ggccacctgc tc
//
LOCUS       DATGFG010000058         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1359783, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000058 DATGFG010000000
VERSION     DATGFG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359783"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2786)
                     /locus_tag="VKQ52_01125"
     CDS             complement(<1..>2786)
                     /locus_tag="VKQ52_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85802.1"
                     /translation="INVSTYYYILTVTNEVGESPASPEVNASPAAPVAVPAAPTGLTL
                     LAGDGLVRISWNDVPGADSYNLYWGTAAGVTPATGTKIPGISSPFLHEALVDGTTYNY
                     VLAAVNAIGEGAASAESNATPAYPVAPPAAPAGLAALAGNGMVTLSWNPVAGATSYNI
                     YWGTVPGVTVKSGNAITGSLNPKWKQTSRNLTAAQLRAAPEYFNQGAWDIIAGARYPQ
                     TMPYSAGLDALRTYLQQFNLPLWQLRQALLPTVGATPAQLLPVAAERFGMSKLYTDIT
                     TNPDFIPRNTAWNTGSQDPVNALVPVSPPFANPSQLSFITAANITYEQMLELLQMSWV
                     QGGLGVSVTGVNDLCMTSDESLSPAPLDARFLDRAHRFLKLWVATGYKMIQLDQLIPN
                     ALVGAGILDGAALIHIAAFRNLQDRTKLSFDQLLSFFQSLDTTIRRNAEGNIILPFYQ
                     QVYQNPSVSAIAPDPDLVAIGSGNPPVDPVLTHHLPAIQAALGISTGDANVLFSLTDD
                     TLTLANLSLMYRVSLLSKQTRLSIPQLLKMALLLNPAAGNFTAAIDGVFAGLASTDAF
                     LSQVVTLQQSGISIDALIYLTTPPASTTLATAIAAADTTVTVTDNSWFPASNFYIQIG
                     AETLLVTAVSGIQNNQWTVTRGQLGTVAAAVGAGALVTATAGWATSVQMTATDIASTL
                     SFVQKAVLTLLSAQTTLVSAITAAQTSFSVAADTGFPVAPFYVSVGAEIMLVTAAGGP
                     GNTNWTVTRGQLGTGPAAAAAGAAVVPAAGDVNGAVISVVAANAHNAAATGVSNDLAA
                     YLLNNLKVPGTGTPLINVLTDNGFINSNAALTSANFPAQFLALQLFDKCALIVRTIKL
                     VVSDLSWLFSHAAVYQGVDFSSLPVTVTQPAQNLNSLLSMILLVKLARLWTSAPPASP
                     VQSLYDIIGGVATGT"
BASE COUNT          580 a          768 c          889 g          549 t
ORIGIN      
        1 gtacccgttg caacgccgcc gatgatgtca tagaggcttt gaacaggcga agccgggggg
       61 gcgctggtcc agagccgggc cagctttacg agcaggatca tgctcaagag cgaattcaaa
      121 ttctgcgccg gctgggtgac ggtcaccggc agcgagctaa agtcgacacc ctggtagacg
      181 gcagcatggg agaaaagcca gctgagatcg ctgaccacca gctttattgt ccggacgatg
      241 agggcgcatt tatcgaaaag ctgcagagcg aggaattgtg ccgggaaatt ggcggatgtc
      301 agcgccgcgt tggaattgat aaatccattg tccgtaagca cattgatcag cggggtcccc
      361 gtaccgggca cttttaggtt attcagcaga taggctgcga ggtcgttcga cacccccgtg
      421 gcggccgcat tgtgcgcgtt agctgccact acggagataa cggctccatt gacatctccg
      481 gcagccggca ccacggcagc tcccgctgct gcggcggctg ggcccgtgcc cagctggccc
      541 cgggtcaccg tccagtttgt attccctggg ccgccggctg ccgttaccaa catgatttcg
      601 gccccaacag atacatagaa gggagccacc gggaaccccg tatcggccgc tacgctgaag
      661 ctcgtctggg cggccgtgat ggcagaaacc agcgtagttt gggcggacag cagcgtcaac
      721 accgccttct gaacaaagct aagcgtactg gcgatatccg ttgccgtcat ctgcacactg
      781 gtggcccacc ccgcggtcgc ggttaccaaa gcgcccgccc cgacagccgc agcaaccgtt
      841 cccaattgtc cccgggtcac cgtccattga ttgttctgta tcccggagac agcggtcacg
      901 aggagggttt ccgcccctat ctggatgtaa aaattggagg ccgggaacca gctgttgtcg
      961 gtgaccgtga ccgtcgtatc cgcggccgcg atggcggtgg ccagggtggt agacgccggt
     1021 ggggtcgtta aatagatgag ggcatcgata gagatgcctg actgttgaag cgtcaccacc
     1081 tgtgacagga acgcgtcggt tgacgcaaga ccggcgaata ctccgtcgat cgctgccgtg
     1141 aaatttcctg ccgcggggtt taacagcagg gccatcttca acagctgggg aatggaaagc
     1201 cgggtctgtt ttgaaagtag ggatacccgg tacatcaggc tcagattggc cagggtcagt
     1261 gtatcgtccg taagggagaa aaggacattg gcatcgccgg tggagatccc gagggctgcc
     1321 tggatagccg gcaggtggtg ggtcaggacg gggtcgacag gcggattgcc cgagccaatg
     1381 gccaccaggt cgggatccgg cgcaatggcg ctcaccgatg ggttttggta cacctgctga
     1441 taaaagggaa gaatgatatt cccttccgca ttcctgcgga tggtggtatc cagcgattgg
     1501 aagaagctga gcagctggtc aaagctaagc ttcgtcctgt cctgcaaatt tcggaaggca
     1561 gcaatatgga tcagcgccgc gccgtcgaga atgcccgcgc caaccaaagc gttgggaatc
     1621 aattgatcca gctggatcat tttgtagccc gtggcaaccc agagtttcag gaatcggtgc
     1681 gcccggtcga ggaagcgcgc atccaagggt gccggcgaaa gggactcgtc gctcgtcatg
     1741 cacagatcgt tgaccccggt gacagatacg ccgagtcctc cctgcaccca actcatctga
     1801 aggagctcga gcatttgttc ataggtgatg ttggcggcgg tgataaagga aagctgggat
     1861 ggattagcaa acgggggtga caccggtacc agtgcgttga cgggatcctg cgaacccgtg
     1921 ttccaggctg tattccgcgg aataaagtcg ggattggtag tgatgtcggt gtacaattta
     1981 gacatcccga atcgctctgc ggctacgggt aatagttggg ccggggtggc gcccacggtg
     2041 ggaaggagtg cctggcgaag ctgccacagc ggcaaattga actgttgaag gtaggtgcgc
     2101 agcgcatcga gaccggcgct atagggcata gtttgcggat acctggcacc cgcgatgata
     2161 tcccaggcgc cctggttgaa atattccggc gcagcccgca gctgggccgc cgtaaggttg
     2221 cgcgaagtct gcttccattt tggattgagt gaacccgtga tcgcatttcc cgacttaacc
     2281 gtcactccgg gaaccgtacc ccaatatatg ttatagctcg tcgccccggc aacggggttc
     2341 cagctcaggg taaccattcc atttccggca agcgccgcga gccccgcagg tgcggcagga
     2401 ggtgcaaccg ggtacgcagg tgttgcattc gactcggcag aagcggcccc ttcaccaatg
     2461 gcattcacgg cagcgagaac ataattgtat gtggtgccgt ctaccagggc ctcatgcagg
     2521 aaggggctgc tgatcccggg tatcttcgtt cctgtggccg gcgtgacgcc ggccgccgtc
     2581 ccccagtaga ggttgtagct gtcggcgccg ggcacatcgt tccagctgat gcggacaagg
     2641 ccgtcaccgg cgagaagcgt cagtcctgtg ggtgccgcag gaacagccac cggtgccgcc
     2701 ggcgaagcgt ttacttccgg cgaggcggga gactcaccca cttcattcgt tactgtgagg
     2761 atataatagt aggtggacac attgat
//
LOCUS       DATGFG010000059         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1127384, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000059 DATGFG010000000
VERSION     DATGFG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127384"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VKQ52_01130"
     CDS             <1..367
                     /locus_tag="VKQ52_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HLZ85803.1"
                     /translation="ELLCILQNVIQRYEPLFKTGFIAESPEKLGRIGEYYTASYGQNP
                     FPYKRCNAPWVSTVIEADGTVRPCFFLPPMGNIHDTPLPEILNGPGAIAFRRSLDIDK
                     NDTCRRCVCYLNLPPRARL"
     gene            364..1845
                     /locus_tag="VKQ52_01135"
     CDS             364..1845
                     /locus_tag="VKQ52_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85804.1"
                     /translation="MTPTLPLRRVLPVLAVYMILSALGLYTHELFLDEAHHFLLARDS
                     ATLTDLYYNARYDGHPRLWHTLLFFITHVLTPNPIAMQVLQGLISAAVAFVFLRYAPF
                     TLPIKIGILFGYYFLFEYNILSRNYALGLLFLFLCCACLANGRRPLLTVGILVLLMCN
                     THIFFTFASIALYGYLFVEYAGERKLFTRPFLIFTGLFLLGFACALVQARVPQVDNVN
                     LTPVKPDKWLSAGNFFFAVFGLVRGWLPIPTANFFWNHFWLSEDRIGRTIGLLLFLCL
                     LLFPALPLRKKPKALLFYYTTVALLLLFFDVTQMTAARYFGMVFIFFLVAAWLSAEGR
                     PDALAGTGPVFRMGLYAILALQLIVGAFAFEQDLAKPFSQSRNTARYLQSLPSGEEIV
                     VDGYNSGPMLCAYLGSKVFYLTTGERGSFCVWKKSYFPTPRPGIGQELAQWPALQKLP
                     RFILVANRKLDTCQNPHFQLSALRFFEKSIEGENYYVYQVNTR"
     gene            1888..3351
                     /locus_tag="VKQ52_01140"
     CDS             1888..3351
                     /locus_tag="VKQ52_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLZ85805.1"
                     /translation="MDKLLFSHSYFMRFDPKQWATGQPYPPLATIGAAALMRGQGYKV
                     SLFDTMFEESAEGVIPHLSAQAPDFFIVYDDGFNYLTKMCLTNMREAAFRMISLAHQQ
                     GCIVIVSSSDSTDHYNAYLDKGADFVLLGEGEETLLELITAIRTGDDNFARINGLAWK
                     KHGLPVQTAARKVMKDLDHLPLPAWDLVDIEKYRRVWMDHHGYFSLNISTTRGCPFKC
                     NWCAKPIYGNRYNARSPRHVVGEIKMLKENYHFDHIWMCDDIFGLKPGWVSEFADLVE
                     QENLHFKFKIQSRADLLLQENYVRALARAGCENSWMGAESGSQKILDAMDKGTTVEQI
                     AEATQLLRRHGIRPSFFIQFGYPGETKEDIDMTIRMINRLLPDSLGISVSYPLPGTVF
                     YSRVKADLKEKTNWTDSDELKLMFHNTYPPAFYKQLHRYVHRSYGWHLALRQLGQLFL
                     QPARLTKRRLRLAASIIYQLPAAIIARQKLKKLAHDY"
BASE COUNT          686 a         1157 c          779 g          745 t
ORIGIN      
        1 cgaactactg tgcatcctgc aaaacgtcat ccagcgctac gagccccttt tcaagaccgg
       61 gttcatcgcc gaatccccgg aaaaactcgg caggataggg gagtactata ccgcctccta
      121 tggccagaac cccttcccat acaaacgctg caatgccccc tgggtctcca ccgtaataga
      181 agccgatggc accgtccgcc cctgtttctt ccttccgccg atgggcaata tccatgatac
      241 gcccctcccg gagatcctga acggccccgg ggcgatcgca ttcagaagat cactcgacat
      301 agacaagaac gatacctgcc ggcgatgtgt ctgctatctt aaccttcccc cccgcgcccg
      361 cctatgaccc caacgcttcc cctgcgccgg gtattgccgg tactggccgt ttatatgatc
      421 ctcagcgcgc tgggcctcta cacccacgag ctgttcctcg acgaggccca tcatttcctg
      481 ctggcccgcg acagcgccac gctcaccgat ctctactaca atgcccgtta tgacggacat
      541 ccccggctct ggcataccct cctcttcttt atcacccatg tcctgacccc gaatcccata
      601 gccatgcagg ttttgcaggg gctcatctcc gcggccgtcg cctttgtctt cctccggtac
      661 gcccccttta ccctccccat aaagatcggc atcctctttg gctactactt tctcttcgaa
      721 tacaatatcc tgagtcgcaa ctatgcgctg ggtctgctct tcctcttcct ttgctgcgca
      781 tgcctggcca acggccggcg gcccctgctc accgtcggca tcctcgtcct gctgatgtgc
      841 aacacccata tcttcttcac ctttgcctcc attgccctct acggctatct ttttgtcgaa
      901 tacgccgggg aaagaaaatt attcacccgc ccctttctta ttttcacggg gctgttcctc
      961 cttggcttcg catgcgccct cgtccaggcc cgcgtaccac aggtggacaa tgtcaacctg
     1021 accccggtaa aacccgataa atggctctcc gccggcaact tcttttttgc cgtcttcggc
     1081 cttgtcaggg gctggctgcc catcccgacc gccaattttt tctggaatca tttctggcta
     1141 agcgaagacc gcatcggacg caccatcggc ctcctgctct tcctttgcct cctgctcttc
     1201 cccgcgctcc ccctccggaa aaagcccaaa gcccttttat tttactatac caccgtggcg
     1261 ctgctcctgc ttttcttcga tgtcacacaa atgacggccg cccggtattt tggaatggtc
     1321 tttatcttct tcctcgtcgc cgcctggctc tccgccgaag gccggcccga cgcccttgcc
     1381 ggtacaggcc ccgtcttccg tatgggcctc tatgccatcc tggccctcca attgatcgtc
     1441 ggcgccttcg cattcgaaca ggacctcgcc aaacccttct cccagtcccg caacaccgcc
     1501 cgctatctcc aaagccttcc atccggcgag gagatcgtcg tagatggcta taactccggg
     1561 cccatgctct gcgcctacct gggcagcaag gtcttctacc tcaccaccgg cgaaaggggc
     1621 agcttctgcg tctggaagaa gtcctatttt cccactcccc ggcccggtat cggccaggaa
     1681 cttgcccagt ggcccgccct ccaaaagctg ccccggttca tcctggtcgc caatcggaag
     1741 ctggatacct gccaaaaccc tcattttcag ctttctgcat tgcgcttctt cgagaagagc
     1801 atcgaggggg agaactatta tgtctaccag gtaaataccc gctgataatc aggcatcccc
     1861 tgcgcggaat tgtccgttta tttcaacatg gataagctcc ttttttctca tagctatttc
     1921 atgcgtttcg atccgaagca atgggccacc ggccagccgt atcctcccct cgccaccatc
     1981 ggcgccgccg cgctcatgcg gggacagggc tacaaggtct ccctcttcga taccatgttc
     2041 gaagagagcg ccgaaggggt gatcccccac ctgtcggccc aggctccgga tttcttcatc
     2101 gtgtacgacg acggcttcaa ctatctgaca aagatgtgcc tcaccaatat gcgggaggcg
     2161 gccttccgca tgatcagcct ggcccatcaa caaggctgca tcgtcatcgt cagcagctcc
     2221 gactccaccg atcactacaa cgcctatctc gataaaggcg ccgacttcgt cctgctcggg
     2281 gaaggagaag agaccctgct cgaattgatc acggccatcc gtaccggcga cgacaacttc
     2341 gcccggatca acggcctggc ctggaaaaaa cacgggctgc ccgtccagac ggccgcaaga
     2401 aaggtgatga aggatctcga tcatctcccc ctgccggcct gggatcttgt cgatatcgaa
     2461 aaatatcgcc gggtatggat ggaccaccat ggctatttct ccctgaatat cagcacgacc
     2521 cgcggttgcc cctttaagtg caactggtgc gcaaaaccca tctacggcaa ccgctacaat
     2581 gcacgctctc cccggcacgt ggtcggcgaa ataaaaatgc tgaaggaaaa ttatcatttc
     2641 gatcatatct ggatgtgcga cgacattttc ggcctcaaac ccggatgggt cagtgaattt
     2701 gccgatctcg tcgaacagga gaacctgcac ttcaaattca agatccagtc ccgcgccgat
     2761 ctcctcctcc aggagaacta cgtccgggcc ctcgcccgcg ccggctgcga gaacagctgg
     2821 atgggcgccg aaagcggctc ccagaagatc ctcgacgcca tggacaaggg caccacggtg
     2881 gaacagatcg cagaagcgac ccaattgctg cgccggcacg gcatcagacc ctccttcttt
     2941 atccagttcg gctatcccgg ggagacaaag gaagatatcg atatgaccat ccgcatgatc
     3001 aaccgcctcc tgcccgacag cctcggcatc tccgtttcct accccctgcc gggaacggtt
     3061 ttttactctc gggtaaaggc cgacctgaag gaaaagacaa attggacgga ctccgacgaa
     3121 ttgaaactca tgtttcacaa cacctacccg ccggccttct ataagcagct gcaccggtat
     3181 gtccaccgta gctatggctg gcacctggcc ctccggcaac tcggtcagct cttcctccag
     3241 cccgcccggc tgacaaaacg aagactgcgg ctggcggctt ccatcatcta tcaactgccc
     3301 gcggccatca tcgcccggca gaaattgaaa aaactggcgc atgactacta gcgacgggat
     3361 caacgaa
//
LOCUS       DATGFG010000060         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_672829, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000060 DATGFG010000000
VERSION     DATGFG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672829"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VKQ52_01145"
     CDS             <1..580
                     /locus_tag="VKQ52_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ85806.1"
                     /translation="HCYDKLDNYAQARFYYRKASHLNPDDSKLYYKLACTYINEEQWG
                     QAVKQLEAAMRIHTMQPEYNLAMGECKMQLGEFKEAIQYFSNVVRIRPRNISSWEALI
                     RCLYNAEYFEEALEQVETAIRITGGKPLFVFYLSAVYFALGRSREGLLQLENAMAKAP
                     KLLKKLLELNPVILQYQPVVDIIARYKKNKSF"
     gene            763..1536
                     /locus_tag="VKQ52_01150"
     CDS             763..1536
                     /locus_tag="VKQ52_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HLZ85807.1"
                     /translation="MNFKLTQMPDRLEKPRQNGITMVMDKGLSIDEARNFLSVAHPHV
                     DIVKLGFGTSFVTPNLREKIEVYQSYGLPVYFGGTLFEAFLIRGQFEDYIAVCKDYGI
                     THMEVSDGSITIPHAEKCGYIEKLTKYGTVLSEVGSKDAAHIIPPYKWIELMSAELSA
                     GAAYVIAEAREAGNVGIYRGSGEVREGLVQEILTQIPAERILWEAPQKAQQLYFIELI
                     GCNVNLGNIAPTEVIAMEAMRVGLRGDTFHLFLDKDAAR"
     gene            1533..2288
                     /locus_tag="VKQ52_01155"
     CDS             1533..2288
                     /locus_tag="VKQ52_01155"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HLZ85808.1"
                     /translation="MRRYGLIGYPLTHSFSQKYFTDKFEREGIKDCVYTNFPISSIEE
                     LPGLLKDGSLCGLNVTIPYKQVVLPYLHGKSEVVEAIGACNCIRIDAGRLVGHNTDVV
                     GFEQSLLPKLGSHHRRALVLGTGGASKAVEYVLGKLGIAYRLVTRRPRPGTDDLSYEE
                     VDAALMASHTLIVNTTPLGMYPHVEECPSLPYEALGPGHYLFDLIYNPARTLFLRNGE
                     ARGAVVENGHEMLLLQAEESWRIWQEPTVSVPE"
     gene            complement(2332..2763)
                     /locus_tag="VKQ52_01160"
     CDS             complement(2332..2763)
                     /locus_tag="VKQ52_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLZ85809.1"
                     /translation="MPRTKLILPDHFSFTTHIPVRITDLNYGGHVGNDSILSIIHEVR
                     VQFLRYHGYQELDMAGVGLIMADVTIEFRSELFYGDTLRASIAAAEFSRVGFDLYYKL
                     EKLTGDKWIPVSNARTGMVCYDYKLKKIAGVPKEVINKLLS"
     gene            complement(2770..3381)
                     /gene="rdgB"
                     /locus_tag="VKQ52_01165"
     CDS             complement(2770..3381)
                     /gene="rdgB"
                     /locus_tag="VKQ52_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HLZ85810.1"
                     /translation="MSHSQTNSESRIRIIFATNNQHKVTEIQSAIGDKLEIISLAQAG
                     IDIDIPEPHDTLEANATEKSSTIHRLTGANCFSEDTGLEVDALDGEPGVLSARYAGED
                     KAFDKNIAKLLQKLGDNPNRQARFRTVISLIWDGREHLFEGISKGHILHAPDGAAGFG
                     YDPVFVPEGDTRSFARMTLEEKNRYSHRRKAADKLIAFLQTQI"
     gene            complement(3418..4236)
                     /locus_tag="VKQ52_01170"
     CDS             complement(3418..4236)
                     /locus_tag="VKQ52_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85811.1"
                     /translation="MLVNGQGAAHHMSLNNIELSGLIVGEWYGDSILVPSADPSALRP
                     VMPAAPTTTTATPTAVTPAPPTAPATTTATPTATTTAPSTAPASSTTTPVPPAPAPVT
                     ARPTPVTPAPAAPSPTPVTPPPTSVRPADASVQPTEPATYKFLGNHRRKVTIIVDSPG
                     TAFLPDDQLNFLTKILEACRMNIGDVAIVNHHTAPVLITPLRRQLQPVHILLFGVDPT
                     TIRLPINFPEFKLQPYDQCTYLSAPALQQLVGNSAESKLLKSKLWVCLKTLFDV"
BASE COUNT          919 a          941 c         1484 g         1083 t
ORIGIN      
        1 gcattgctat gataaattgg ataactatgc gcaggcccgg ttctattacc ggaaggcttc
       61 gcatctcaat cctgacgaca gcaagctgta ttataaattg gcttgtacct atatcaatga
      121 ggagcaatgg gggcaggcgg tgaagcaact ggaggccgcc atgcggatcc ataccatgca
      181 gcccgagtat aatttagcga tgggcgagtg caagatgcag cttggggagt tcaaggaggc
      241 cattcagtat ttttccaatg tggtgcggat ccggccccgg aatatctcca gctgggaggc
      301 gttgatccgt tgcctgtaca atgccgagta ttttgaggag gcgttggagc aggtggagac
      361 ggcgatccgg atcactgggg gtaaaccctt atttgtcttc tatctttctg ccgtatattt
      421 tgcccttggg cggagtcgcg aagggctttt gcaattggag aatgcgatgg caaaggcgcc
      481 aaagctgttg aagaaattac tggagttgaa tccggtgatc cttcaatacc agcccgtggt
      541 agacattatc gcgcggtata agaagaataa atcattctaa agaaccccga cagggtttga
      601 aggctaacca atgttttaat gatattctaa taatgttgca tacgaccgga caggttggta
      661 tgcgatagtt tcctattttt gcgccggtta ctaagaattt gcgcagtgag ggattagtgg
      721 ggagcaggta tataatgtta acagactcaa taaaagaaaa ggatgaattt caaactcacg
      781 cagatgccgg acaggctgga aaagccccgg cagaatggca ttaccatggt catggacaaa
      841 gggctcagca tcgatgaagc aaggaatttt ttgagtgtgg cgcatcccca cgtggacatc
      901 gtcaaattgg gttttggtac ttctttcgtg actcccaatc tccgggagaa gatcgaggtt
      961 taccagtctt acggccttcc ggtctatttt ggcggtactc tttttgaagc tttcctgatc
     1021 cgtgggcaat tcgaagatta tattgccgtc tgcaaggact atggcattac ccatatggag
     1081 gtgagcgatg gcagcatcac catcccgcat gccgagaaat gcgggtatat cgagaaactg
     1141 acgaagtacg gcacggtgct cagtgaggtg ggtagcaagg atgccgcgca tatcattccg
     1201 ccgtataaat ggatcgagct gatgtctgcc gaactgagcg cgggggccgc ctatgttatc
     1261 gccgaggcga gagaagcggg gaatgtgggg atctatcgtg gcagcggcga agtgcgggag
     1321 ggtcttgtgc aggagatact gacgcagatt cccgccgaac gcatcctgtg ggaagctccc
     1381 cagaaggcgc aacaattgta ttttatcgag ctgatcggct gtaatgtcaa cctcggcaat
     1441 atcgctccca cggaggtgat cgctatggag gccatgcgtg tcgggctgcg tggcgatacg
     1501 ttccatcttt tcctcgacaa agacgccgcg cgatgaggcg ctatgggctg atagggtatc
     1561 cgctgacgca ttctttttcg caaaagtatt ttacggataa gtttgaacgg gaggggataa
     1621 aagactgtgt ctatacgaat tttcctattt cgagtatcga agaattaccc ggactgttga
     1681 aggatggatc gctttgcgga ctcaatgtca cgatcccgta taagcaggtg gtgctgccct
     1741 atcttcatgg gaagagcgaa gtggtggagg ccatcggggc ttgtaactgc atacgcatcg
     1801 atgccgggcg gcttgtgggg cataataccg atgtggtggg gtttgagcag tcgttgctgc
     1861 cgaagctggg cagtcatcat cgccgggcgc tggtcctggg gacgggtggg gcttccaagg
     1921 ccgtggagta tgtgcttggg aagttgggga ttgcctatcg gctggtgacg cggcggcccc
     1981 ggccgggtac ggatgatctt tcttatgagg aggtggatgc ggcgctgatg gcatcgcata
     2041 cactgatcgt caatactacg ccgctgggga tgtatccgca tgtggaggag tgtccgtccc
     2101 tgccttatga ggcgcttggg ccggggcatt acctgttcga cctgatctat aacccggcga
     2161 ggacgctgtt cctgcggaac ggggaggcgc ggggggccgt ggtggagaat gggcatgaga
     2221 tgctgctcct gcaggcggag gagagttgga ggatatggca ggagccgacg gtttcggtgc
     2281 cagagtaacg gggattgacg cgggcgtggg tgatcgcagc gagatcgcag tctaggacag
     2341 caacttattg atcacttctt ttggaacgcc tgctattttc ttcaatttat agtcataaca
     2401 gaccatgccg gtgcgggcgt tgcttaccgg tatccacttg tcgccggtga gtttttccag
     2461 tttataatac agatcgaagc ctacgcggga gaattcggcg gcggcgatgg aagcgcggag
     2521 cgtgtcgcca tagaacagtt cgcttctgaa ctcgatggtg acatcggcca tgatgaggcc
     2581 tacgccggcc atgtccagtt cctggtagcc atgatagcgg aggaattgga cccttacctc
     2641 atgaatgatc gagaggatgg agtcgttgcc gacatgaccg ccatagttga ggtcggtgat
     2701 gcggaccggg atatgcgtag taaatgaaaa gtggtcaggc aggatcaatt tggttcttgg
     2761 catatgcaat catatttggg tttgtaggaa ggcgatgagt ttatccgcgg ctttccggcg
     2821 gtggctatag cgatttttct cctccagggt catccgggca aaggagcggg tgtcgccttc
     2881 ggggacgaag accgggtcgt agccaaagcc ggcggcgccg tcgggggcgt ggaggatatg
     2941 gcctttgctg atgccttcga agagatgttc ccggccatcc cagatgagcg agatgacggt
     3001 gcggaagcgg gcctggcggt tgggattgtc gccgagcttt tggagcagtt tggcgatgtt
     3061 cttgtcaaac gctttgtctt cgccggcata gcgggcgctg agtacgccgg gttcgccatc
     3121 gagggcgtcc acttcgaggc ccgtatcctc gctgaagcag ttggcgcctg tgagccggtg
     3181 gatggtggaa gacttttcgg ttgcgttcgc ctcgagggta tcatggggtt cggggatatc
     3241 gatatcgatg ccggcctgcg cgagactaat gatctcgagt ttgtcgccga tggcggactg
     3301 gatctcggtg accttgtgct ggttgttggt agcgaagatg atgcggatgc gggattcgct
     3361 gttagtttgg gagtgactca tacaggttgg ggatggttca tataggttgg gaatggctca
     3421 tacgtcgaat aaggttttca ggcagaccca gagtttgctt ttcaggagtt tgctttccgc
     3481 ggagttccca accagttgtt gcagtgcggg cgccgagaga taggtgcatt gatcataggg
     3541 ttgcagtttg aattccggga agttgatggg taaccggatg gtggttgggt cgaccccgaa
     3601 cagaaggata tgtacgggtt gcagctgccg gcgcaggggg gtgatgagga ccggcgccgt
     3661 atggtggttg acgatggcca cgtcgccgat gttcatccgg caggcttcga ggatcttcgt
     3721 gaggaagttg agctgatcgt cggggaggaa ggcggtgccg ggggagtcga cgatgatggt
     3781 gaccttgcgg cggtggttgc cgaggaattt ataggtggcg ggttccgttg gttggacgga
     3841 cgcgtcggcc ggcctgacgg atgtgggggg tggggtgacg ggtgtgggtg acggggcagc
     3901 gggtgcgggg gtgacgggtg tgggccttgc cgtgacgggt gcgggtgccg ggggtacggg
     3961 tgtagtggtg gatgatgcag gcgcagtgga tggcgcagtt gtggtggcgg ttggcgttgc
     4021 ggttgttgtc gccggggcag tgggtggcgc gggtgtgacg gcggttggcg ttgcggttgt
     4081 tgttgtcggc gcggcgggca tgacggggcg cagcgccgaa ggatcggcgg aggggacgag
     4141 gatggaatcg ccgtaccatt ccccaacgat aagaccggat agttcaatgt tgttgagact
     4201 catatgatgt gcggccccct gaccgtttac taacaattgt tagtttttga ttttgttctc
     4261 acaaagattg ttcgtttctt tgtgtcaaca aaatttaccc agtatgattg caaccctgga
     4321 catccaagtt acaaaaacgg ggaagagtaa agttagcgag atcgatttta acaatctccc
     4381 ttttggcaaa tattttaccg accatatgct ggaagtggat tatgcgg
//
LOCUS       DATGFG010000061         3990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_334786, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000061 DATGFG010000000
VERSION     DATGFG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334786"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..513
                     /locus_tag="VKQ52_01175"
     CDS             253..513
                     /locus_tag="VKQ52_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85812.1"
                     /translation="MKKQVVSMVKGFLFFTMPALCFAKYFFIEYGNVRLGQGYNFPSS
                     FLLAIGGWFLTGLRKGEGTGSRYPLVFATASGFQDSPTLVFR"
     gene            521..1960
                     /locus_tag="VKQ52_01180"
     CDS             521..1960
                     /locus_tag="VKQ52_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ85813.1"
                     /translation="MTTTPKHLFRLEVKRRSDRLMNYFLPGFFLLGFIFSVFYDTWGI
                     ALGVGGFSLLAFYGTRMILPESDLYQYVLSGVLGIFMAQYIYQMHGMFEMHFFAFIGS
                     AILITYQNWKLQIPILLVVLVHHAIFGYLQNTGYDKVYFTQLNYFELRTFVIHIGLSA
                     IIFFTCGLWSYQLSRYSEAQITQAIELGRIEQEALRVEEGRRQEAQRQSEALEKAVAQ
                     GKFEMAADVLHDIGNAVVGFGSYLVRVRRLQESSQTNRLQQLSELFRTERVSLVAALG
                     EARASAIATLLDGVSRMQRSNEEELGRSVTEQSGILARIEEILHIQRHYIQGREGQER
                     RPVNLGAVLGDCLAIQQAAMAKRGIEVHSDIDGEMPAIRGDQTKLMQVILRMLKNSIE
                     AMELSAGERRLSVRLAVRAGMLVLELEDTAPHLRVAGRETADGLGDKGLGWDSCRAIV
                     ESHGGVLTMAGEEKGSVTTLAFAVEGKVG"
     gene            complement(2011..>3990)
                     /locus_tag="VKQ52_01185"
     CDS             complement(2011..>3990)
                     /locus_tag="VKQ52_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ85814.1"
                     /translation="SSLGGNNIYSIACDRDNDLWLGAFGNCLDRYDPQTNGFRHYRHD
                     PANSNSLSSARIHTLLADAEGYLWVGTFDGGLDRFDKQTGIFTHYIHSDTRNSLSNNS
                     VNCICEDSRHDLWIGTANGLDRLDRQTGRFTTWRTTDGLPNNMIYGILEDKARNLWIS
                     TNNGIARFDRNTGAFRIFTSADGLQSNEFKPHSCTETATGRLYFGGVRGFNEFDPDSV
                     TIHPAGSPLVITGFQLFNREVPVSTGNLPTPLKKDITETDHLTLEYSNTVISFDFARL
                     NYYAPEKNRYAYQLEGFDTAWNDIGSRHMATYTRLDPGDYTLRIKTRGNNGDWDPAVK
                     SVAITINPPFWKTWWFRALLVLAIIGAVTGAHAIRLRSLQHQKNKLEDQVAKLLDRAV
                     AQGKHEMASEVLHDIGNAIIGFSSHVHRIKRLLETNNTQSLFSLAGFFRDHRAHFDKA
                     IGEQKTGAIVNLLEGIAKSQKEGHEEMNRAVTEQYNTTVRIQEILHIQRQYLTGQDSQ
                     ERKPIPLQKVISDATAMLSATFQKHGITVDFQPGNEPLVVKGDRTRLMQLLLNLLKNS
                     IEALHTTSGEKLISIRLCRRQDDLHIQIRDSGGGFDEDTAGRLFVKGFSTKPSGGGVG
                     LFQCRTILDSHNGNLVLASDGPGKGCLVTVIFQAA"
BASE COUNT          775 a          916 c         1355 g          944 t
ORIGIN      
        1 tcagggtatg atcggagacg tcgatggttg tcgcccctgt gaaggatcgg gcggcggcgg
       61 aaagggaacc cctgacgtcg agggtggaag tcggagaagc ggtgccgatg ccgacctgtg
      121 caagggtgga cagggaaagc aggcatccca gtaaaaaagt aaaatactgt ttcatatttc
      181 aggcgttatt ttagatggag gatgagaagg gaaagcaaca gggagaaaga agggaaaact
      241 aaaatacgac cattgaaaaa acaggtggtg tccatggtga aggggttttt attttttact
      301 atgccggctt tatgttttgc taagtacttt tttatagaat atggaaatgt gaggctgggg
      361 cagggctata acttccccag ttctttttta ttagctatag gtggctggtt tctgaccgga
      421 ctgcgaaaag gtgaaggaac cggtagtaga tatccattag tttttgctac ggcaagcggg
      481 ttccaagact cgcccactct tgtttttcgt tgataagggt atgacaacca ctccaaaaca
      541 tctgttccgg ctcgaagtga agcggcgatc ggaccggctg atgaattatt ttcttcccgg
      601 cttcttcctg ctgggtttca tcttttcggt attctatgac acctggggga ttgccctggg
      661 ggtaggcggg ttctcgctgc tggcgtttta tggaacccgg atgatcctgc cggagtccga
      721 tctataccag tatgtgctca gcggggtgct gggcattttt atggctcagt acatctacca
      781 gatgcatggg atgttcgaga tgcatttctt tgcctttatc gggagcgcca tcctgatcac
      841 gtaccagaac tggaaattgc agatcccgat cctgctggtg gtgctggtac atcatgccat
      901 ctttggttat ctgcaaaata cgggttatga caaggtgtat ttcactcaat tgaattattt
      961 cgagctgcgg acctttgtta ttcatatcgg gctttccgcg atcattttct ttacctgcgg
     1021 gctgtggagt taccagcttt ccaggtacag cgaggcgcag atcacgcaag ccattgaatt
     1081 gggccggatc gagcaggagg ccttgcgtgt cgaggagggc cgccggcagg aggcgcagcg
     1141 gcagtcggaa gctttggaaa aggcggtagc acagggaaag ttcgagatgg ctgccgatgt
     1201 cctgcacgat atcgggaatg ccgtggtggg attcgggtcc tacctggtgc gggtgcggcg
     1261 gctgcaggag agcagccaga cgaacaggtt gcagcaattg tcggagcttt tccggacgga
     1321 gcgggtttcc ctcgtcgccg cgcttggaga ggcgagggca agcgccatcg ccaccctgct
     1381 ggacggggtc agcaggatgc aacggagcaa cgaagaggaa ctgggccgtt cggtaaccga
     1441 acaatcgggc atccttgcgc ggatcgagga aatactgcac atccagcggc attatatcca
     1501 gggcagggag gggcaggagc ggcggccggt aaacctgggc gccgtccttg gcgattgcct
     1561 ggcgatacaa caggcggcca tggccaagcg gggtatcgag gtgcatagtg atattgacgg
     1621 ggagatgccg gccatcaggg gagatcagac caagctgatg caggtgatcc tgcggatgct
     1681 gaagaacagt atcgaggcga tggaattatc ggcgggcgag cggcggcttt cggtccggct
     1741 ggccgtaagg gccggaatgc tggtgctgga gttggaggat accgcccccc atttgcgcgt
     1801 tgcgggccgg gaaacggcgg atgggttggg ggataagggg ctgggttggg atagctgccg
     1861 ggcgatagtg gagagccatg ggggtgtgtt gaccatggcg ggtgaggaga agggatcggt
     1921 gacgacgctg gcgtttgcgg tcgaggggaa agtgggttga tcgccaggcg tttgcggacc
     1981 ggggagagtg ggttgatggg ctggggttgc tcaggcggcc tggaagatga ccgttacgag
     2041 gcagcccttg ccgggtccgt cgctggccag tacgagattg ccgttgtggc tgtcgaggat
     2101 ggtgcggcat tggaaaaggc cgacgccgcc gccggagggt tttgtcgaga atccttttac
     2161 gaagagccgg ccggccgtat cctcgtcgaa gccgccgccg ctgtcgcgga tctggatgtg
     2221 gaggtcgtcc tggcggcggc acaggcggat ggagatgagc ttttcccccg atgtcgtatg
     2281 cagtgcttcg atgctgttct tcaggagatt gagcagtaat tgcatgagcc ttgtccgatc
     2341 gccttttacg accaggggtt cattgccggg ttggaaatcg acggtgatgc cgtgcttctg
     2401 aaaggtggcc gacagcatcg cggtggcgtc gctgatgacc ttttgcaagg ggatcggttt
     2461 tctttcctgg gagtcctggc ccgtgaggta ttgccgctgg atgtggagga tctcctggat
     2521 cctcaccgta gtattatatt gttcggtgac ggcgcggttc atttcttcgt gaccttcttt
     2581 ttgtgatttg gcgatgcctt ccagaaggtt gacgatagcg cctgtcttct gctcgccgat
     2641 ggccttatcg aaatgggcgc ggtgatcgcg gaagaagccc gcaaggctga agaggctttg
     2701 ggtattgttt gtctccagca gtctttttat ccggtgaaca tggctgctga agccgatgat
     2761 ggcgttgccg atgtcatgca ggacctccga ggccatttcg tgtttgccct gggcgacggc
     2821 ccggtcgagc agttttgcga cctggtcttc gagtttgttc ttctggtgtt ggagcgatcg
     2881 gagccggatg gcgtgtgcgc ctgttacggc gccaattatg gccagcacga ggagggcccg
     2941 gaaccaccag gtcttccaga agggcgggtt gatcgtgatc gcaacgcttt tcaccgcagg
     3001 gtcccagtcg ccgttgttgc cgcgtgtttt tatcctgagg gtatagtcac cggggtcgag
     3061 gcgggtatag gtggccatat ggcggctgcc gatatcgttc caggccgtgt cgaagccttc
     3121 caactggtat gcataccggt tcttttccgg ggcgtagtag ttgagcctcg caaaatcgaa
     3181 ggagatgacg gtgttggaat attcgagggt cagatgatcg gtctcggtga tatctttttt
     3241 cagcggtgtt gggaggttac cggtagaaac gggcacttcc ctgttgaaca actggaaacc
     3301 cgtgatcacc aatggtgagc ccgcgggatg gatcgtgacg ctgtcggggt caaattcgtt
     3361 gaacccgcgg acgccgccga aatacaggcg gccggtggcg gtctccgtac aggaatgggg
     3421 tttgaactcg ttggattgca gaccatcggc cgacgtaaag atgcgaaagg cgccggtgtt
     3481 ccggtcgaac cgggcgatgc cgttgttcgt gctgatccag aggttgcggg ccttatcctc
     3541 caggatgcca tagatcatgt tgttgggtag tccatccgtc gtccgccagg tggtgaagcg
     3601 acccgtttgc cggtcgaggc gatcgaggcc gttggcggtg ccgatccaaa gatcatggcg
     3661 gctgtcctcg cagatgcagt tgacggaatt attgctgagg ctgttgcggg tgtcggagtg
     3721 gatataatgg gtgaagatgc cggtctgttt atcgaaccgg tcgagtccgc cgtcgaaggt
     3781 gccgacccac agataaccct cggcatcggc caggagggta tggatgcggg cgctgctcag
     3841 gctgttcgag ttggcaggat catgccgata gtgccggaaa ccatttgtct gcgggtcata
     3901 ccggtcgagg cagttgccga aggcgcccag ccagaggtcg ttgtcgcggt cgcaggcaat
     3961 ggagtaaata ttattgccac cgagggagct
//
LOCUS       DATGFG010000062         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1589811, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000062 DATGFG010000000
VERSION     DATGFG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589811"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VKQ52_01190"
     CDS             <1..363
                     /locus_tag="VKQ52_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLZ85815.1"
                     /translation="TVTATDTNNCKAIDSVLIKTICNSDNIFMPNTFSPNGDGVNDVF
                     YPRGKSLYNVQSLTVFNRWGQMVFQRSDFPANTQSMGWDGNFNGHPAPADAYVYIVEV
                     ICENAQVVAIHGTITLVR"
     gene            393..1427
                     /locus_tag="VKQ52_01195"
     CDS             393..1427
                     /locus_tag="VKQ52_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HLZ85816.1"
                     /translation="MRRHKLYSFLLLFLLAASARAQDIHFSQFYETPLLRNPSLAGIF
                     TGDYRIQTVFRDQWNSFADAYRTGSFNAEFKWPVGNQKDYFTTALQALYDKAGTAALT
                     TTEILPAINYHKSLSEQRNTYLSLGFMGGLVEKTLDLSKVTTNSQFNGTAYDPALPNG
                     ETILSPNVHYLDGSLGISFNTSYGPDNQNTMYLGFAYHHLNRPKNSFYKNPTIELDPK
                     IVFSGGLKTVVNDFSYFTLYADYSRQGPATETIGGVLYTYKLGDDPDNYPYAFTLGAM
                     LRWNDALIPVVKLEMNSFNVGLSYDVNVSRLNTVSNSRGGFELSLSYTGFLDRNQDRT
                     SSGKYHDPRF"
     gene            1455..1934
                     /locus_tag="VKQ52_01200"
     CDS             1455..1934
                     /locus_tag="VKQ52_01200"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLZ85817.1"
                     /translation="MSIPSIQVGQQAPDFTLYNSEKEKVTLSDYKGRNVLLLFFPQAF
                     TGTCTKELCSTRDNIALYNKANAQVFGISVDSVFTLARFKEDQQLNFPLLSDFNKTVS
                     AEYGTLYDTFVLDMRGVSKRSAFLIDKAGIVRYAEVLETASDLPDFAAIQKALVDLN"
     gene            2103..2405
                     /locus_tag="VKQ52_01205"
     CDS             2103..2405
                     /locus_tag="VKQ52_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ85818.1"
                     /translation="MTSIQAKSQARGPLSEPEHKVIKFYPNPAVSYITFELTKEQNKA
                     YTLQIFSFLGRKVKEVPDMADKKTVNLSDLTRGLYTFQLKDEDGRVTDSGIFQVNK"
BASE COUNT          656 a          772 c          496 g          525 t
ORIGIN      
        1 actgtaacgg cgacggatac caacaactgt aaggccatcg acagcgtcct gatcaaaacc
       61 atctgcaaca gcgacaacat ctttatgccg aatacctttt cgcccaatgg cgacggcgtt
      121 aacgacgtct tctacccacg cggcaaaagc ctgtataatg ttcagtccct gaccgtcttc
      181 aaccgctggg gacagatggt cttccaacgg agcgattttc cggccaacac ccagagcatg
      241 ggctgggatg gaaatttcaa cggccacccc gcaccggcgg acgcctatgt ctatatagtg
      301 gaagtgattt gcgagaacgc acaggtggta gccatccacg gaacaataac cctggtgcga
      361 tagctgttcg ataaaaccaa atagtattcc gattgagacg acataaatta tatagcttcc
      421 ttctcctctt cctcctggcc gcgtccgcac gcgcacagga tatccatttc tcccagttct
      481 atgaaacccc actcctgcga aacccttccc tggcagggat cttcacagga gactaccgca
      541 tccagaccgt cttccgcgac cagtggaaca gcttcgccga cgcctatcgc accggctcct
      601 tcaacgcgga gttcaaatgg cccgtcggca accagaaaga ttatttcacc accgcactgc
      661 aagccctcta cgataaagcc ggcaccgccg cccttaccac caccgagatc ctgccggcta
      721 tcaactatca taaatccctc agcgagcagc gaaatacata cctctccctt ggcttcatgg
      781 gcggcctggt ggaaaaaaca ctcgatctgt cgaaagtcac caccaacagc cagtttaacg
      841 gcaccgccta tgatccggcc ctcccaaacg gcgagaccat cctctctccc aacgtacact
      901 atctcgacgg tagcctcggc atcagcttca acacatcata cggccccgac aaccagaata
      961 cgatgtacct cggcttcgcc tatcaccacc tcaaccggcc aaagaactcc ttctataaga
     1021 accccaccat cgaactggac cccaagatcg ttttctccgg cggcctcaaa accgtcgtca
     1081 acgacttttc ctattttacc ctctacgccg actactcacg ccagggcccc gctaccgaaa
     1141 ccatcggcgg cgttctctat acctataaat tgggcgatga ccccgacaac tacccctatg
     1201 ccttcaccct cggcgccatg ctccgctgga acgatgccct catccccgtg gtgaaactgg
     1261 agatgaactc cttcaacgtc ggcctcagct atgacgtcaa cgtatcccgg ctcaacacgg
     1321 taagcaacag ccgcggcggc ttcgaattgt ccctctccta tacaggattt ctcgatcgta
     1381 accaggacag gaccagcagc gggaaatatc acgatccgag attttagcct acatttacgc
     1441 cctaaaataa acgtatgagc atcccatcca tccaggtagg ccagcaagcc cctgatttta
     1501 ctctctataa ttccgaaaaa gaaaaagtta ccctgagcga ctacaaaggc agaaacgtcc
     1561 tgctgctgtt cttcccccag gccttcaccg ggacatgtac aaaagagctc tgcagcaccc
     1621 gcgataacat cgccctctac aacaaggcca acgcccaggt gttcggcatc agcgtcgact
     1681 ccgtcttcac cctggccagg tttaaagaag accagcaact caacttcccc ctgctcagcg
     1741 attttaacaa aaccgtctcc gccgaatacg gcaccctcta cgacaccttc gtcctggaca
     1801 tgagaggtgt ctccaaaaga tcggccttcc tcatcgataa agccggtatc gttcgctatg
     1861 cggaagtcct ggaaaccgcc agcgacctcc ccgatttcgc cgcgatccag aaagccctgg
     1921 tcgatctcaa ctaattaata atcaattaaa tacaaaaagg ccggtcaacc cccggccttt
     1981 tattttgttg tattaatctc cgccctaatt tgtatctttg aaaagatatg actattttgg
     2041 aaccacgtgt aaaatatacc taaagtatga ggatttttta cattttgtta tccattattc
     2101 taatgacatc catccaggct aaaagccagg caaggggccc attgtccgag ccggaacata
     2161 aggtcataaa gttctatccg aaccctgcag tctcctatat caccttcgaa ttgaccaaag
     2221 aacaaaacaa agcctataca ctgcagatct tcagcttcct cggaaggaag gtgaaagaag
     2281 ttccggatat ggccgataaa aaaacggtca acctgagcga ccttacccgt ggcctctata
     2341 ccttccagct gaaggatgag gacggcaggg tcaccgactc aggcatcttc caggttaaca
     2401 agtaagaaaa gccactctga tatctccacg ataaccaccg caggtacag
//
LOCUS       DATGFG010000063         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_106119, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000063 DATGFG010000000
VERSION     DATGFG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106119"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..739
                     /gene="lepB"
                     /locus_tag="VKQ52_01210"
     CDS             71..739
                     /gene="lepB"
                     /locus_tag="VKQ52_01210"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLZ85819.1"
                     /translation="MKPLILTVLLPGLIFCLWVIGRVTHAFQFYRASTSSGEPALKPG
                     ELFFISNWKTPKRFDIIAYRMVIAVQGANTLVHRICGVPGDVVEIKGGTLFVNGKDVD
                     ACLRLKHIYKVSRRYAGSFGFKEEDAYTIPPYTDTLYIPLEDRTVREEQLPCTRYILP
                     AGLRDEEILKSYQKSWNQDNFGPLRVPPDRFFVLGDNRGKSLDSRYIGLIDQQKVVGT
                     VLWK"
     gene            complement(784..2052)
                     /locus_tag="VKQ52_01215"
     CDS             complement(784..2052)
                     /locus_tag="VKQ52_01215"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLZ85820.1"
                     /translation="MFRPILLFLLASQTFMTSQALPAAQTLPAAPAPTFRNGQWRALL
                     IRKDGNDIAFNFDVKDSAGKQILYLRNAGERILVDDITRDGDSLFIKFPFYESQLRAR
                     VTPEGNLEGVWLRHLAADWLTVPFKAFFGEAYRFANPPFGNGPIITGRWPAVFRSTTN
                     ADTTFRIGEFQQTGSRVTGTFLDAGGDLRYLEGSISGDSLRLSCFDGTHAYYFTAKID
                     GNNLTNGHYYAGLTAHETWTAHKDPNAKLEDQFAMTKWKKDMPFTFTFRDLDGNNVSL
                     SDPRFKNKVVIVQLMGSWCPNCMDETRFLSGFYNEYRNKGVEIIALAYERSTDFQRSQ
                     ASVRTFQQRFQVQYPMLIIGAAVGDPQRAEKTLPQIERIVNFPTTIFVGKSGHIEKIH
                     TGFSGPGTGQHYEDQKVEFYTLVNDLLAAK"
BASE COUNT          431 a          538 c          689 g          501 t
ORIGIN      
        1 tgctgaagaa gatcgaggta ttgctgcatg ggtgcaattt acgtatttta gggacgataa
       61 accgttgtac ttgaaacccc tcatcctgac agtcctccta ccgggcctta tcttctgcct
      121 ttgggtcatc ggccgggtga cccatgcatt tcaattttac cgggcgtcca cttcgtccgg
      181 agagcccgcc ttaaaacctg gcgaactctt ttttatctcg aactggaaaa ccccaaaacg
      241 gttcgatatc atcgcctatc ggatggtgat cgcagtgcag ggcgccaaca cgctggttca
      301 ccgcatctgc ggcgtccccg gggatgtcgt ggagataaaa ggcgggacgc tttttgtcaa
      361 cgggaaggac gtcgatgcgt gcctgcggtt aaagcatatt tacaaggtga gccggcgata
      421 cgcggggtct ttcgggttta aagaggagga tgcctatacg atcccgccat ataccgacac
      481 cctgtatatt ccgctggagg accgtaccgt tcgggaggag caattgccct gtacgcgtta
      541 tatccttccg gccgggctcc gggatgagga gatcctcaag agctatcaga agagctggaa
      601 ccaggataat tttgggcccc tccgcgtacc gccggacagg ttcttcgtcc ttggcgacaa
      661 ccgggggaaa tcgctggatt cccggtatat cgggttgatc gatcagcaga aagtggtggg
      721 tacggtgttg tggaaatagc cggtcgggcg attgggtggc tgatgccggc ggcggtggtg
      781 atgttatttc gccgcgagga gatcatttac gagggtgtag aattctactt tctgatcttc
      841 ataatgctga ccggtgcccg gaccgctgaa gccggtgtgg atcttttcga tgtgcccgct
      901 cttccctaca aagatcgtag tgggaaaatt gacgatccgt tcgatctggg gcagggtctt
      961 ttccgcgcgt tgcggatcgc cgacggccgc cccgatgatc agcatgggat actgcacctg
     1021 gaaacgttgc tggaaggtgc ggacgctggc ctggctgcgc tggaagtcgg tgctgcgttc
     1081 atacgcaagg gcgatgatct ccactccttt attgcgatat tcattgtaga atccgctgag
     1141 gaagcgggtc tcgtccatgc aattgggaca ccagctgccc atgagctgga cgatcacgac
     1201 cttgtttttg aaccgcgggt ccgacaagga gacgttgttg ccgtcgaggt cccggaaggt
     1261 gaaggtgaac ggcatgtctt tcttccattt tgtcatcgcg aactggtcct ccagttttgc
     1321 gttggggtcc ttatgtgccg tccaggtctc gtgggccgtg agaccggcgt agtagtggcc
     1381 gttggtcagg ttgttgccgt cgatctttgc cgtgaagtaa taagcgtgtg tcccgtcgaa
     1441 gcaggagagc cttagcgagt ccccgctgat gctaccttcg aggtagcgga ggtcgccgcc
     1501 ggcgtcgagg aaggtgccgg tgacgcggga accggtttgc tgaaactccc cgatgcggaa
     1561 ggtggtgtcc gcgttggtgg tggaacggaa aacggcgggc cagcgaccgg ttatgatggg
     1621 gccattgccg aacgggggat tcgcgaaccg gtaggcctcc ccgaaaaatg ccttgaaagg
     1681 aacggtcagc cagtcggccg ccaggtgccg gagccagacg ccttcgagat tgccttcggg
     1741 agtgacgcga gcccgcagtt gcgattcata gaaggggaat tttatgaaaa gcgagtcgcc
     1801 gtcccgggtg atatcatcca ccaggatgcg ttcgccggca ttgcggagat agagaatttg
     1861 tttgcccgca ctgtccttta catcgaaatt gaaggcgatg tcgttgccgt cctttcttat
     1921 cagcagggcg cgccattggc cgttgcgaaa ggtcggagca ggggcggccg gcagggtctg
     1981 ggcggccgga agggcctggg aggtcatgaa ggtttgcgag gccagcagga acaggaggat
     2041 cggtctgaac atagcgttaa ctcgttaagg gtgcaaggta acgaatttaa tcgatatcag
     2101 tctattaaat cagtagagtg ttcggcatac ccgggggccg gcctattcca ggttcccga
//
LOCUS       DATGFG010000064         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1358955, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000064 DATGFG010000000
VERSION     DATGFG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358955"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /gene="purL"
                     /locus_tag="VKQ52_01220"
     CDS             complement(<1..1299)
                     /gene="purL"
                     /locus_tag="VKQ52_01220"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HLZ85821.1"
                     /translation="MEITAKTAEQLRLTAEEFGLIQGKLGRTPNFTELCAFSAMWSEH
                     CSYKNSIKWLKTLPREGKKMLVKAGEENAGLMDIGNGHGVVFKIESHNHPSAIEPFQG
                     AATGVGGIHRDIFTMGARPIASLNSLRFGNIKEAKTQHLLSGVVHGIGHYGNCFGVPT
                     VGGEIYFEECYHTNPLVNAMSVGILKNGETISATAKGKGNPVIFVGSATGKDGIGGAS
                     FASANITGESTKELPAVQVGDPFQEKKLLEACLEVIHTGAVVGMQDMGAAGIICSTAE
                     MSAKGEVGMRIDLDRVPARQENMKAWELLLSESQERMLMVVEKGREAEVVGIFEKWDL
                     PAAAIGEVTDDGLLKFYMHGELEAEIPAHELVLGGGAPQYDRPYSEPGYLRQVKAFDA
                     AQVAVPEDLRAVAEQLIALPNIASKKWVAVQYDSTVGAANT"
     gene            1368..2522
                     /locus_tag="VKQ52_01225"
     CDS             1368..2522
                     /locus_tag="VKQ52_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLZ85822.1"
                     /translation="MAVNGRLAFVANTSWSIYKFRLYLIERLLEEGFAIFVLAPRDPY
                     TEKFEKLKGLTYVGLHHFHGKSISPVQDFLLYRELFRHYRAIRPQLIFHYTVKANLYG
                     TRAAARAGIPSVSVITGLGYTFAGKGWLQRGVRIGYRRELQKSTEVWFLNTDDRHIFT
                     SQHLVPPEKTFLLPGEGVDAGHFQPTPDEPATHKPQKEAVTFLLIGRLIRHKGIYEFV
                     QAAKQLQQQGLAVRCQLLGFFDDNNPVAIPREQVEEWTARKIVTYLGQTDDVRPFIAQ
                     ADCIVLPSYREGMPLSLLEGASMGKALIATDTAGCRAIVEEGVNGYLCREKDSHHLAT
                     KMAAYYHLPPAAKEQMGLEGRKLVLQRFTREIVAAVYLDKIKTLRATGHE"
BASE COUNT          542 a          840 c          612 g          532 t
ORIGIN      
        1 cgtattggcg gcgccaaccg tgctgtcgta ttgcacggcg acccatttct tcgaagcgat
       61 gttcggcagg gcgataagct gttccgcgac tgcccgcagg tcctccggca ccgccacctg
      121 tgcggcatca aacgccttca cctgccgcaa atacccgggt tcactgtacg gccggtcgta
      181 ctgcggcgca ccaccaccca gcaccagttc atgcgcaggg atctcggcct cgagttcgcc
      241 atgcatatag aatttgagca ggccgtcgtc cgttacttcc ccgatcgccg ccgccggcag
      301 atcccatttc tcgaagatcc ctaccacttc ggcctccctg cctttttcca cgaccatcag
      361 catccgctcc tggctttcgc tgagcaacag ctcccaggcc ttcatattct cctggcgcgc
      421 gggtacccgg tccaggtcga tccgcatgcc tacttctccc ttggcgctca tctcggcagt
      481 ggaacagatg atccccgcgg cgcccatatc ctgcatcccg acgacagcac cggtatggat
      541 cacctccagg caggcttcca gcaatttctt ctcctggaaa ggatcaccca cctgcacggc
      601 cggcagttcc ttcgtgctct cacccgtaat attggccgaa gcaaaagaag ctccgccgat
      661 accgtcctta cccgtggcgc tcccgacaaa aatcacggga ttgcccttac cctttgccgt
      721 ggccgagatc gtctccccgt tcttcaggat accgacgctc atggcattca ccagcgggtt
      781 cgtatgataa cactcttcga aatagatctc tcccccgacc gtcggcaccc cgaaacaatt
      841 gccgtaatgc ccgatgccat gtacgacacc cgacaacagg tgctgggtct tcgcttcctt
      901 tatattgccg aaacgcaggg aattcagcga ggcgatgggc cgggcgccca tggtaaagat
      961 atcgcggtgg atacccccca ccccggtagc cgccccctgg aaaggctcga tggccgaggg
     1021 atggttatgt gattcgatct tgaaaacgac tccgtgtcca ttgccgatat ccatcaaccc
     1081 ggcattctcc tccccggcct ttaccagcat cttctttccc tcccggggca gggtcttcag
     1141 ccacttgatc gagttcttat aactacagtg ctcgctccac atggcgctaa aagcgcataa
     1201 ttctgtaaaa ttgggtgtac ggcctagctt tccctggatc aaaccgaatt cctccgctgt
     1261 caatcggagc tgttcggccg tttttgcggt aatttccatg gccgcaaagg tacaatccgc
     1321 ttcccaatct tattgctttt tatggaaaat cagtacgttt gcctcttatg gcggtaaacg
     1381 ggagacttgc ctttgtagca aacaccagtt ggagtatcta caaattcagg ctttatctta
     1441 ttgaacgact gctggaagag gggtttgcca tctttgtgct ggcgccccgg gatccctata
     1501 cggagaaatt cgaaaagcta aaaggattaa cctacgtcgg actgcatcat tttcacggga
     1561 aaagcatctc ccccgtgcag gacttcctgc tctaccgcga actgttccgc cactaccgcg
     1621 cgataagacc gcagctcatc ttccattata ccgtaaaagc caacctgtac ggtacccggg
     1681 cagccgcccg cgccggcatc ccctccgtca gcgtcatcac gggccttggt tataccttcg
     1741 ccggaaaagg ctggttgcaa cgcggcgtca ggatcggcta tcggcgcgaa ctgcaaaaga
     1801 gcaccgaggt ctggttcctc aataccgacg accggcatat ctttacctcc caacacctcg
     1861 tcccgccgga aaagaccttt ctcctgccgg gcgaaggcgt ggacgccggt catttccagc
     1921 ccacacccga tgaacccgca acccataaac cccaaaaaga agccgtcacc ttcctgctca
     1981 tcggccggct gatccgccac aaggggatct atgaattcgt ccaggccgca aaacaactgc
     2041 aacaacaggg cctcgccgtc cgttgccagc tcctgggttt cttcgacgac aataatcccg
     2101 tcgccatccc gcgcgaacag gtagaggaat ggacggcccg caagatcgtt acctatctgg
     2161 gccagaccga cgatgtccgc cccttcatcg cacaggcgga ttgcatcgtt cttccctctt
     2221 accgcgaagg gatgcccctc agcctgctgg aaggagcaag catgggcaag gcgctgatcg
     2281 ccaccgacac cgccggctgc cgggccatcg tcgaagaagg cgtcaacggc tatctctgcc
     2341 gcgaaaaaga cagccaccac ctggcgacaa aaatggccgc ctattatcac cttccgcccg
     2401 ccgccaaaga acagatgggc ctcgaaggcc gtaagctcgt cctgcaacgg tttacccgcg
     2461 agatcgtggc agccgtctac ctcgacaaaa taaaaacact ccgggcaacc ggtcacgaat
     2521 aaacac
//
LOCUS       DATGFG010000065         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_104785, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000065 DATGFG010000000
VERSION     DATGFG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104785"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..489)
                     /locus_tag="VKQ52_01230"
     CDS             complement(52..489)
                     /locus_tag="VKQ52_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HLZ85823.1"
                     /translation="MSSVETTTTGHHEQPHFDTKPIWRTFWILLGITMVELMLATIHL
                     KTGFPNKYVLNGVYLILTCAKAFYIIAEFMHLRHEIKNMIMTIAVPALLFVWFIVAFL
                     WDGASYRELRNRYDQHHAEEYKVKVAPEGGESAPPAQKPGSPE"
     gene            complement(510..1184)
                     /locus_tag="VKQ52_01235"
     CDS             complement(510..1184)
                     /locus_tag="VKQ52_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HLZ85824.1"
                     /translation="MAQAIPAGQKWWSGGKSPFNVEYGKLMMWYFLMSDAFTFGAFLI
                     SYGSIRFASNGWPDPNEVFRAFPLMGGLHLPLVFVSLMTFILIMSSVTMVLAVAAGHA
                     GDQKKVTFYMILTIIGGLMFLSCQAWEWTHLHSEGAWWGRNPFPNADGSKASTNFSNF
                     FFTITGFHGFHVFSGVVINTIMLIKNLRGDFARRGHYLMIEKAGLYWHFVDLVWVFVF
                     TCFYLL"
     gene            complement(1231..1809)
                     /locus_tag="VKQ52_01240"
     CDS             complement(1231..1809)
                     /locus_tag="VKQ52_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HLZ85825.1"
                     /translation="MSELIMDIVNEQRKKIHPHKFTLWVGLGSIIMMFAGLTSAYVVK
                     RDQPGWTSFFIPRAFWYSTAVILISSLTIQMALKAFKDREMLRYRGLMSATAVLGIVF
                     VVLQWVGFRAIWDSGINFHGSGGGQFLYVIAGLHAVHVLGGIIPLVVMVGRAYATKLR
                     SYNSIPVEIMSTYWHFVDLLWLYLFIFFMAIK"
     gene            complement(1806..>2354)
                     /locus_tag="VKQ52_01245"
     CDS             complement(1806..>2354)
                     /locus_tag="VKQ52_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HLZ85826.1"
                     /translation="LAAALSAFSLFIYAFVYTPLKKVSAISVLVGAIPGSLPCLIGWA
                     AGNDDLSIGGWVLFALQFFWQFPHFWAIAWIAHKDYSTAGFKLLPSNEGPTKYSAIQA
                     IIYSLLLIPVGVLPYFLGGEHGGMSGILALVIVLAANLAMVAQCVRLYREMTVKAARR
                     VMFSSYIYLPVVLLSLLANKNV"
BASE COUNT          615 a          660 c          577 g          502 t
ORIGIN      
        1 gccttcggcg acggtccctt tttcatttat ttcggttctg cgtgttaaat cttactcggg
       61 cgatccgggc ttttgagcgg gaggtgcgga ttcaccgcct tcgggcgcca ctttcacctt
      121 gtattcctcg gcatgatgct gatcatagcg gttacgcaac tcccggtagg aggcgccatc
      181 ccacaggaag gcgacgataa accatacaaa gagcagcgcc ggcacggcga tcgtcatgat
      241 catattcttg atctcatgac gcaggtgcat gaactcggca ataatataga aggccttggc
      301 gcaggtgagg atcaggtaaa ccccattgag gacatatttg ttggggaatc ccgtcttcag
      361 gtgaatggta gccagcatca gctccaccat cgtgatcccc agcaggatcc agaatgtcct
      421 ccagatcggc ttggtatcga aatgcggttg ttcgtgatga cccgttgttg ttgtttcgac
      481 gctagacata taataatttt taattgagtt tagagtaagt agaagcaggt gaacacgaat
      541 acccatacca ggtcgacgaa gtgccagtac agaccggcct tctcgatcat caggtaatgg
      601 cctctccggg caaaatcccc gcggaggttc ttgatcagca tgatcgtatt gatgacgaca
      661 cccgagaata cgtggaagcc gtggaaaccc gtgatggtaa agaagaaatt gctaaaattg
      721 gtcgatgcct tcgatccgtc ggcgttgggg aaaggattcc ggccccacca ggcgccttca
      781 ctatgcagat gcgtccattc ccaggcctgg cagctaagga acatcaatcc gccgataatg
      841 gtcaggatca tataaaaggt cacctttttc tgatcacccg cgtgaccggc cgcaaccgca
      901 agcaccatgg tcaccgagga catgatcagg ataaaggtca tcaggctcac aaacacgagg
      961 ggcagatgca aaccacccat caacggaaag gcacgaaaca cttcattggg gtcgggccag
     1021 ccgttgctgg caaaccggat agaaccataa gagatcagga aagcaccgaa agtaaaggca
     1081 tcactcatca gaaaatacca catcatcagt ttcccatact ctacgttgaa gggactctta
     1141 ccgccgctcc accatttctg acctgccggt attgcttgtg ccatgttttg tttttagttg
     1201 atttgcaaat ttatttaaca atactgaaac ttattttatc gccataaaga aaatgaacag
     1261 gtacagccag agaagatcta caaaatgcca gtaggtgctc atgatctcca ccggtatact
     1321 attataactg cgcagcttcg tagcataggc ccttcctacc atcaccacca ggggaatgat
     1381 cccgcccagc acgtgtaccg catgaagccc tgcaatgaca taaaggaact gtccgccgcc
     1441 actcccatgg aaattgatgc ccgagtccca gatcgcccgg aagccgaccc attgcaacac
     1501 cacaaagacg atccccagta cggccgttgc actcatcagg cctcgatagc gcaacatttc
     1561 ccggtcctta aaagccttca gggccatctg gatggtaaga ctgctgatca ggatcaccgc
     1621 ggtcgaatac cagaaagccc tcgggataaa aaagctcgtc cacccgggct gatcccgctt
     1681 gaccacatag gcgctggtca gcccggcaaa catcatgatg atgcttccca atcccaccca
     1741 taacgtgaat ttatggggat gtattttctt tcgttgctcg ttcacaatat ccataattaa
     1801 ctcgctcaca catttttatt cgccagcagc gataataaca ccacaggcag atagatataa
     1861 ctactgaaca ttaccctcct cgcggccttc acggtcatct cccggtagag ccgcacacat
     1921 tgcgccacca tcgccaggtt ggccgccagc acgatcacca gggccaggat cccgctcatt
     1981 ccgccatgct cgccgcccaa aaaatagggc agcacgccca ccgggatcag caacagcgaa
     2041 tagatgatgg cctggatagc ggaatatttt gtcggtccct cattggacgg cagcaacttg
     2101 aaacccgccg tcgaatagtc cttgtgcgcg atccaggcga tcgcccagaa atgcgggaac
     2161 tgccagaaga actgcaacgc gaacaatacc cagcccccga tggagaggtc gtcattaccc
     2221 gccgcccagc cgatgaggca ggggaggctt ccgggtatgg cgcccaccag gacggagatg
     2281 gcgctcacct tcttcagcgg cgtatagaca aacgcgtaga tgaagaggct gaaagctgac
     2341 agtgccgccg cgag
//
LOCUS       DATGFG010000066         3623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1472823, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000066 DATGFG010000000
VERSION     DATGFG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472823"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..437)
                     /gene="panD"
                     /locus_tag="VKQ52_01250"
     CDS             complement(93..437)
                     /gene="panD"
                     /locus_tag="VKQ52_01250"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HLZ85827.1"
                     /translation="MDIEILKSKVHRAVITEANLNYVGSLTLDEDLMDAANMIENEKV
                     QIVNVNNGSRIETYLIKGKRGSGTCCLNGPAARQGAVGDIVIIISYAHMDFEEAKQFK
                     PRIVFPKEGNKL"
     gene            complement(527..1633)
                     /gene="panC"
                     /locus_tag="VKQ52_01255"
     CDS             complement(527..1633)
                     /gene="panC"
                     /locus_tag="VKQ52_01255"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HLZ85828.1"
                     /translation="MILFKQSADLATWLEKQRMAGRNIGFVPTLGALHEGHISLIAIS
                     KKSTSLTVCSIFVNPTQFNDPRDFQQYPITLEKDIPMLEKAGVDVLFLPAVAEMYPNG
                     TGGLETYDLGPLETLLEGKYRPGHFQGVCQVMRRLLEKVRPDHLFMGSKDYQQCMVVQ
                     RLLTIMGFPTTLHRCPIIREADGLAMSSRNLRLTPTQRTNATAIYRALTAIKNGFPAQ
                     PIPTLIANATRILEAAEFRVDYVSIADAQTLEPLGDLTTPAASAITTTSPSSSATTSP
                     PTTATATNTTSPPSSAATSPAASATTGPSSSARTAPALSAPPANTPSSPRPTTYSSEA
                     ATDVPPPPDGAVALIAAFQGDVRLIDNRVLSEPS"
     gene            1924..2736
                     /locus_tag="VKQ52_01260"
     CDS             1924..2736
                     /locus_tag="VKQ52_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HLZ85829.1"
                     /translation="MLQKKRILFIANEMSPYLELTEFAEIVNKLAIKSNDNGFEVRCI
                     MPRFGVVNERRHRLHEVVRLSGINVSVDNDDFPLQIKVASLPNARLQVYFLDNEDLFK
                     RKFIFHDENDKWFEDNGLRTVFYCKGALETVKKFGWPPDVIHCSGWMTGLIPLYLKTV
                     YKKEPVFAHSKCVFTIGQPTFKEKLGADFLKIANIHANIKDKDLEPYKEANNTALFRG
                     GATYADAITFGAEKVDKKLVEEFGKVRGRKVLSFNAESDLTDYLQLYMDLSK"
     gene            2831..>3623
                     /gene="metK"
                     /locus_tag="VKQ52_01265"
     CDS             2831..>3623
                     /gene="metK"
                     /locus_tag="VKQ52_01265"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLZ85830.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDHFLAFDPQSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQDIARDVIRRIGYTKSEYMFEANSCGIFSAIHEQSSDI
                     NQGVDRKKKEEQGAGDQGMMFGYASNETEDYMPLALDLAHKILLELAALRRENKSIKY
                     LRPDAKSQVTLEYDDNNKPVRIDAIVVSTQHDDFDTEAKMQARIKKDIIEILIPRVKA
                     KYKKYARLFNEQIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGG"
BASE COUNT          842 a          785 c         1097 g          899 t
ORIGIN      
        1 cttagactca accacacaaa aaacgctgcc agcgcagcaa aaaatacgta ttgcaggaag
       61 acgaaaattt ttttccgcat agccacgaca agttacagtt tatttccttc tttcggaaaa
      121 acgatacggg gtttgaactg ctttgcttct tcgaaatcca tgtgtgcgta agagatgatg
      181 atgacgatat cgcccactgc accctgcctt gcagcggggc cgttcaggca gcatgttccg
      241 gagccgcgtt ttccttttat gagataggtc tcgatacgtg agccgttgtt gacgtttacg
      301 atctgaactt tttcgttttc gatcatgttc gccgcatcca tcaggtcttc atcgagtgtg
      361 aggcttccta catagttgag gttggcttct gtaatgacgg cgcggtgaac ttttgatttc
      421 aggatctcga tatccatagg tgatttttga aagttgcttt ttgttttaaa agggcacgaa
      481 agtatacaat tttagcaaac gaaaggttcg gtcgggcgaa cggcgtttag ctcggttcgg
      541 acagaaccct gttatcgatg agccggacat ccccctggaa ggcggcgatg agagcgacgg
      601 cgccatcggg gggtggaggg acgtcggtgg ccgcctcgga tgagtaggtg gtggggcggg
      661 gcgaggatgg ggtgtttgcg ggtggagcgg agagggccgg ggcagttcgc gcggaggaag
      721 acgggccggt ggtcgcggat gcggcggggg aggttgctgc ggaggaaggt gggctggtag
      781 tgttggtcgc ggttgcagtt gtgggggggg aggttgtggc ggaggaagat gggctggtgg
      841 tggtgatcgc ggatgcggcg ggggtggtga ggtcgccgag gggttcgagg gtttgggcgt
      901 cggcgatgga tacgtagtcc acccggaatt ccgcggcttc gaggatgcgg gtggcgtttg
      961 cgatgagggt ggggatgggt tgggcgggga agccgttttt gatggccgtg agggcccggt
     1021 agatggcggt ggcgttggtg cgttgggtgg gcgtgaggcg gaggttgcgg ctgctcatgg
     1081 ccaggccgtc ggcctcgcgg atgatggggc agcggtggag ggtggtgggg aagcccatga
     1141 tggtcaggag ccgttgcacg accatgcatt gctgataatc cttgctgccc atgaagaggt
     1201 gatcggggcg tactttttcc aggagccggc gcatcacctg gcagaccccc tggaaatgcc
     1261 cgggccggta tttgccttcc aggagggtct cgagggggcc gagatcatag gtttcgaggc
     1321 cgccggtgcc attggggtac atttcggcga ccgcggggag aaagaggacg tcgacccctg
     1381 ctttttcgag catggggatg tctttttcca gggttatggg gtattgctga aaatctcttg
     1441 ggtcattgaa ctgggtaggg ttgacaaaga tgctgcaaac cgttagggat gtgctttttt
     1501 ttgatatagc aataagtgaa atatgtcctt catgaagggc gcccagggtg ggtacgaagc
     1561 cgatgttccg gccggccatg cgttgctttt cgagccaggt ggccaggtca gcggactgtt
     1621 tgaataaaat catgatttgg caattttggc aaaaaaggag caaaatataa ttttttgcca
     1681 aaacagggct aataacgggg cccggattcc atccggcctg gtgttttacg ctccaaaacc
     1741 ggctacagtg ccgattttac aataaaaatt gtacctttgc aaactatttc gatttgtttt
     1801 gcgcctttgg gttggtgtgc aaaataaccc ttcggcgcaa agcgatcaag gatggccttt
     1861 ggcgggcgag ccaacggctg ccttatccag ttaaggttcc tctcaacaat tattaaataa
     1921 ataatgctac aaaaaaagcg aattttattt attgccaacg aaatgtcgcc ttatttagag
     1981 ctgactgaat ttgcggagat cgttaataag ctggctatta aatcaaatga caatggattc
     2041 gaggtgcgat gcataatgcc gaggtttggt gtagtgaatg aaaggaggca tcggcttcat
     2101 gaagtggttc ggctttccgg gatcaatgtt tctgtggata atgatgattt cccgttgcag
     2161 atcaaagtag catcccttcc caatgcccgt ctccaggtgt atttcctgga caatgaggac
     2221 cttttcaagc gtaaattcat ctttcacgac gaaaatgata aatggtttga agacaatgga
     2281 ctgcgtaccg tcttctactg taagggcgcc ctggagaccg tgaagaagtt cggctggccg
     2341 cccgatgtga tccattgcag cggctggatg acggggttga tcccgcttta tctgaagacc
     2401 gtttataaga aggagcctgt ttttgcccac agcaaatgtg tatttaccat cgggcagccc
     2461 acctttaaag aaaaattggg ggcggatttc ctcaagatcg ctaacattca cgccaatatc
     2521 aaggataagg atctcgagcc ttataaggaa gccaataaca cggcgctctt ccgtggcggc
     2581 gccacctatg ccgatgccat tacctttggc gccgagaagg tggataagaa actggtggag
     2641 gagttcggca aggtccgcgg ccggaaggta ctctccttta atgcagagtc tgatttaaca
     2701 gattatttgc agttgtacat ggacctgagt aaataaccct aatgggttga atttttctgc
     2761 ggcaatccct tacctttgcc gcgtcagtta ttaaataact cattaatatt catcaaacct
     2821 tttaaaaaaa atgccttatt tattcacttc tgagagtgtt tccgaagggc atccggacaa
     2881 ggtggccgac cagatctccg atgcattgat cgatcatttc ctggctttcg atccccagtc
     2941 gaaagtcgct tgtgaaacgt tggtgacgac gggtcaggta gttctggccg gtgaagtaaa
     3001 atctaaggca tacctcgacg tacaggacat cgcccgggat gttatccggc gtatcggtta
     3061 caccaagagc gagtacatgt tcgaagccaa ttcctgtggt attttctccg ccatccatga
     3121 gcaatcctcc gatatcaacc agggtgttga ccggaagaag aaggaagagc agggtgcggg
     3181 cgaccagggg atgatgtttg ggtatgccag caatgaaacc gaggattata tgccgctggc
     3241 gctcgacctg gctcataaga tactgctcga actggctgcg ctgcgtcgcg agaacaagtc
     3301 gatcaagtat ttgcgtcctg atgccaagag ccaggttacg ctggaatatg acgacaacaa
     3361 caagccggtg aggatcgatg cgattgtcgt atctacccag cacgatgact ttgacaccga
     3421 agccaagatg caggccagga tcaagaagga tatcatcgag atcctgatcc cccgggtaaa
     3481 ggcaaaatac aagaaatacg cgcggttgtt caacgagcag atcaagtatc atatcaaccc
     3541 caccgggaag tttgttatcg gtggtccgca cggcgatacc gggcttaccg gccggaagat
     3601 catcgtcgat acctatggcg gca
//
LOCUS       DATGFG010000067         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_786529, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000067 DATGFG010000000
VERSION     DATGFG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786529"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VKQ52_01270"
     CDS             complement(<1..1023)
                     /locus_tag="VKQ52_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLZ85831.1"
                     /translation="MFQRITVVLLGIVLFGWWPAMAQNDVVKDDSASVFNRNRPDRVE
                     WFRTVGEGLFIHFGVDAQLGIVISHSLVGASDDYVNRYFHELPATFDPSRFDPRSIAR
                     LAKLAGMKYIVFTTKHHSGFCLWDTRTTAFNCMNTPCHRDMLKEYVEATRAEGLGVGL
                     YFSPEDFHFLYTHGLPINRGVPAFDAATRAEYDDYTRRQCGELMTNYGPIDVLFIDGD
                     PKDVVKKTCWDLQPGLLITRGAMQTPEQVLPGTRITDPWLSCITMGTAWQYQPTNERY
                     KSGTRLIGLAVEARAKGGSLLLNVGPKPDGELAAEQEDRLREMAAWYFVNHECMDSVN
                     SWVVPRE"
     gene            complement(1028..2572)
                     /locus_tag="VKQ52_01275"
     CDS             complement(1028..2572)
                     /locus_tag="VKQ52_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ85832.1"
                     /translation="MQKLNKGILLAAMAATVLTSCDKGYKELNINPDAVSTPTPEYVF
                     TLAEYEGAGYGGTYGSSAADFLLGTMQYTTSYNDVAGFGSKYINSQVQQTGNAFSAAY
                     PDEINELGIVIKAVKSDPAKINWLAEARIWRVYCFMRQTDLYGDIPYSQAGLGYDSSN
                     YKPVYDPQQTIYSNMLSELDAAAQSLDAGKSTFSAADLIYGGNTAQWKKFAYSLMLRL
                     GMRMTKVDPASAQTWVTKAIAGGVITQDADIAKMSYLKGGQTINENPLVLDLWNNDYI
                     AQNGQTNTEGGKYADTWISYLQSTNDPRLPIISVVWIGGKPDTTSSKQLGMSQDIPNK
                     IPANFVSFSEPNPATVLGLGSPKLLFTAAESYFLMTEAALRGWYNGATASSLYTLGIQ
                     AAMRHWSIVAGSAGTISQAAINTYVNNNTLVSGTLDQQMKQIYTQFWVSMFPDAQEVY
                     NNWRRTGYPALVPNNYPTNATGGQIFRRFEYPISEQSLNTVNYNAAIARQGADDLMTR
                     IWWDKQ"
BASE COUNT          527 a          756 c          756 g          611 t
ORIGIN      
        1 ttccctggga acgacccagg agttgacgct gtccatgcat tcgtggttga caaaatacca
       61 ggccgccatc tcgcgcaggc ggtcctcctg ttccgcggcc agttccccat cgggttttgg
      121 tccgacattc agcaggagcg agccgccttt tgcccgggct tccacggcca gcccgatcag
      181 gcgggttccg ctcttatacc gttcgttcgt cggctgatac tgccaggctg ttcccatcgt
      241 gatacaggag agccagggat cggtgatgcg ggtcccgggc aggacctgct cgggtgtttg
      301 catcgcgccc cgcgtgatga gcagacccgg ctggagatcc cagcaggtct tcttcaccac
      361 gtcctttgga tcgccgtcga tgaacagcac gtcgatgggg ccgtagttgg tcatcaattc
      421 cccgcactgc cgccgggtgt agtcgtcata ctccgcacgg gtggcggcgt cgaaagccgg
      481 caccccccga ttgataggca acccatgtgt gtaaaggaag tgaaaatctt ccggtgaaaa
      541 ataaagccct acccccaggc cttcggccct tgtcgcctcc acgtattcct tcagcatatc
      601 ccggtggcag ggcgtgttca tacaattgaa ggcggtggtc cgcgtatccc agagacaaaa
      661 accggagtga tgctttgtcg taaagacgat gtacttcatt cccgccagtt tcgccaatcg
      721 cgcgatgctc cggggatcga agcgggatgg gtcgaaggtg gccggcaatt catgaaaata
      781 gcggttcaca taatcatccg aagcgcccac cagggagtgg ctgatcacga tacccagttg
      841 ggcatccacc ccgaaatgga tgaagagccc ctcccccacc gtgcgaaacc attccacccg
      901 gtcgggccgg ttacggttga atacgctggc gctatcgtcc ttcaccacat cgttctgcgc
      961 cattgccggc caccagccga agagtacgat ccccaataac acaacggtta tcctctggaa
     1021 catgcggcta ttgtttgtcc caccagatac gcgtcatcag atcatccgcg ccctgacggg
     1081 cgatggccgc attatagttg acggtattca ggctttgttc ggagatagga tattcaaatc
     1141 tccggaagat ctgcccgccc gttgcattgg tcggatagtt gttggggaca agcgcgggat
     1201 agcccgtgcg ccgccagttg ttgtagactt cctgggcatc cgggaacata ctcacccaaa
     1261 actgggtata gatctgtttc atttgctgat cgagggttcc ggagacgagg gtgttgttat
     1321 tcacatacgt gttgatcgcc gcctgggaga tggtcccggc gctgcctgcg acgatggacc
     1381 aatgccgcat ggccgcctgg atccccagcg tatacaggga gctggccgtt gccccgttat
     1441 accagccccg cagggcagcc tcggtcatca ggaaatagga ttctgccgcc gtgaacaaca
     1501 gcttcggcga acccaggcca agcacggttg caggattggg ctcggaaaaa ctgacgaagt
     1561 tggcgggtat cttgttgggg atgtcttgcg acatgcccaa ttgtttggaa gaagtagtat
     1621 ccggtttccc gccgatccat accacggaga taatgggcag ccggggatca ttcgtcgatt
     1681 ggagatagct gatccaggtg tccgcgtatt tgcccccttc cgtattggtc tggccgttct
     1741 gggcgatgta gtcattgttc cagagatcca gaaccagggg gttctcattg atcgtctggc
     1801 cgcccttaag atagctcatc ttcgcgatgt cggcatcctg ggtgatcacc ccgcccgcga
     1861 tggcctttgt cacccaggtc tgggccgaag ccggatccac ctttgtcatc cgcatgccca
     1921 gccggagcat cagcgaatag gcaaacttct tccattgcgc tgtattaccg ccatagataa
     1981 gatcggcggc gctgaaggtg ctcttgccgg cgtcgagact ctgcgcggcc gcgtccagct
     2041 cggaaagcat gttggaatag atggtttgct gggggtcgta tacgggcttg taattggagg
     2101 aatcataccc caggcccgcc tgggaatacg ggatatcgcc ataaagatcg gtctgccgca
     2161 taaagcagta cacgcgccag atgcgggctt cggccagcca gttgatcttg gccggatcgg
     2221 acttcaccgc cttgatcacg atacccagct cattgatctc atcgggataa gcggcgctga
     2281 aggcattgcc cgtctgctgt acctggctgt tgatgtattt ggaaccaaac ccggccacat
     2341 cgttatagct ggtagtgtac tgcatggtgc ccagcaggaa gtcggcggcg ctggagccat
     2401 aagtgccgcc ataaccggct ccctcatatt ccgccagcgt gaacacatac tcgggtgtcg
     2461 gcgtgctcac ggcgtccggg tttatattga gctccttata tcctttgtcg caggatgtca
     2521 gcaccgtcgc cgccatcgcc gccaggagga ttcctttatt tagcttttgc attgtcattg
     2581 ttgttgtttt taaattgggc gatcctgttt agaatttaac ggcaaggtta agaccataac
     2641 ttctcgtgcg
//
LOCUS       DATGFG010000068         5700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1471907, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000068 DATGFG010000000
VERSION     DATGFG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471907"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VKQ52_01280"
     CDS             <1..1468
                     /locus_tag="VKQ52_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLZ85833.1"
                     /translation="YVLRRKQRVSGRFSRWLSRIFLQEKLYNWFGCLLLGIIAVGFGY
                     LLAKQVIVGMSLLAVVIGLCAVLPCLANGATALYFILIYNYFVYALPRLVGDNTLKVG
                     IGYDVLIVCGMLGFLFRRESLRQASNHLFRTPVMIWTVVLYLYICIEFFNPFAHSFAG
                     WYDTFRRTTESFLLFFLAYHVFNSWKRIDRFLLVLFWVTFLVALYGCIQQWHGLFSFE
                     MTYISKIKGTSLYYAGQIRKFSTTSGPTAFGMDMAATAVLYIILSINEHRRSRRYLYL
                     IGVIIMIVSSTYSGTRTSNIMLLAGLGVYVLLMADKKSTRIFAGVALFLLIAAIKMPF
                     YGSPTLNRFRSTFEGKHDESYLVREVNRKRIQPYIYSHPIGGGLGTTNAAGLAYNPGH
                     YLADFQTDDGYLKTALEIGSIGLLITCMLYFMVLRTGVSEYFNTSIERNKSVISACLA
                     CIFSFMLADLAQEGINEITNVAIYYPAIAILLRANYVT"
     gene            complement(1465..3186)
                     /locus_tag="VKQ52_01285"
     CDS             complement(1465..3186)
                     /locus_tag="VKQ52_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLZ85834.1"
                     /translation="MGDPKQIPNLNTNGVEANSYDAIVIGSGISGGWAAKELCEKGLK
                     TLVLERGRNVRHIKDYTTAFMAPWEFPHHLENTRKNKEEDPIQSGAYSESNKQFFVSD
                     REHPYIQEKPFNWIRGYQVGGRSLTWGRQCYRLSDLDFEANAKDGIAVDWPIRYKDIA
                     PWYDYVETFVGISGKPEGLSHLPDGVFLPPMEMTCIETEFAQKIKEKYADRIVTMGRV
                     ANLTQGWKGRGPCQYRNLCTRGCPFSGYFSSNAATLPAAELTGNMTLLPDSIVVEILY
                     DEQKRRASGVRVMNAHTKTITEYFARIIFLNASTIPTAAILLNSTSPAFPKGLGNSSE
                     QVGHNLMDHFTGSGAQGEYEGFADQYYSGRRPAGIYIPRFRNLGTSTARKDFIRGYGL
                     QGFGARLEGVEQLATLEGFGPGFRDKLIQPGPWYLWMGGWGETLPYYSNKVTLDTEKK
                     DRWGQPVIKIDFEYHENERAMQKDVEESTREMLERTGFKNINAYSHLQPGGSAVHEMG
                     TARMGRDPKTSVLNSFNQMHDVKNVFITDGSCMTSSATQNPSLTYMALTARACDYAVT
                     EMKKGNL"
     gene            complement(3193..3771)
                     /locus_tag="VKQ52_01290"
     CDS             complement(3193..3771)
                     /locus_tag="VKQ52_01290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HLZ85835.1"
                     /translation="MKRRTAIGRILLTGVIGAGAFSGYKWYEWNKSPDFGYLDTHRAT
                     LAALVQTILPPGPSIPGAADAGVHDYIIRIVKDCAGIHTANVFIDGLKDLAHYSRANY
                     TKEYETCTPEQQTAILKHFEEKGRPMDGILGKAENKFLGKAFFYQLRQYTVQGYCTSE
                     LGAGKGLAYVAIPGSFHGCIPKTQGQRAWATK"
     gene            complement(3773..4534)
                     /locus_tag="VKQ52_01295"
     CDS             complement(3773..4534)
                     /locus_tag="VKQ52_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ85836.1"
                     /translation="MKREKSIRPAGSYLLIALMLLTAFATQAQTESEPPRSPYTPPLS
                     KRPIRSLPIKDTGVEEKLVILALQNVAYDAAIRTVTLAHYKVKQEKNSWFDLLQLNSQ
                     FNDQSFKHQSTVGNVAYVYPKYFYGVSIPIGTILSKGGQVKAAKEGVKIAEDNQIEAA
                     LKLRTDVLSKYKGWRVSNSLVLLERQVADDIHAAFLQVEKRFNDGTVSIEAYSEASRN
                     YSAEMTKLLNYQLQADLQKLEIEQIIGVRLESVIN"
     gene            complement(4589..>5700)
                     /locus_tag="VKQ52_01300"
     CDS             complement(4589..>5700)
                     /locus_tag="VKQ52_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLZ85837.1"
                     /translation="DLDFDIQESTKYIDSLQAKINILRGTAAQDASKEVAVETLMKDA
                     EQANKDYLDAKKRYNDAIDYTSSSLNNFRQVVLGQPAIVPEPSKKILIVALAAVGSAI
                     TAILIILILAYLDSSVKTPRLFARVVNLKLISMINFMNLKQKTISELVTALDTSYDPK
                     DKQRHNVFRESLRKLRYEIEISGKKIFLFTSTKKGQGKTTIIQALAYSMSMSKKKILI
                     IDTNFCNNDLTVALEAPPILEKIVPNDRTDESLAEQIRNLSKDIGVDTVFIIGSEGGD
                     YTPSEILPRKHLLQHLKSLSHDYDFIFLEGPPLNDFSDSRELAQYVDGVIGIFSADQI
                     IKQIDKESLEFFQELNGKFSGAILNKVDLEMVNVS"
BASE COUNT         1033 a         1317 c         1754 g         1596 t
ORIGIN      
        1 ttatgtactc cgcagaaaac agcgggtcag cgggcgattc agccgttggc tatcgaggat
       61 ctttctccag gagaagctct acaattggtt cggctgcctg ttattaggga tcatcgcggt
      121 cgggtttggc tatcttcttg ccaagcaggt gatcgtcgga atgagccttt tggccgtggt
      181 gatcgggctt tgcgccgtac tgccttgtct ggccaatggg gcgacggccc tgtattttat
      241 cctgatctat aactactttg tttatgccct tccgcgactg gtgggagata atacgctaaa
      301 ggtcgggata gggtatgacg tgctgatcgt ctgcggcatg ctcggatttt tgttcaggag
      361 ggaatccctg cggcaggctt cgaatcatct gttccggacg ccggtgatga tctggaccgt
      421 cgtattgtac ctgtacatct gtattgaatt tttcaatccg tttgcccact cttttgccgg
      481 ctggtatgat actttccggc gtactaccga gagctttttg ctgttctttc ttgcttatca
      541 cgtttttaac agctggaaac ggatcgatcg attcctgctg gtgttgttct gggtcacgtt
      601 ccttgtcgca ctgtatggct gcatccagca gtggcatggg ttattctcct tcgagatgac
      661 ctatatcagc aagatcaagg gtacttcgct ttactatgcc gggcagatac gcaagttctc
      721 gaccacttcg ggtccgacgg cgtttggcat ggatatggcc gccactgcgg tgctatacat
      781 catcctgtcg ataaacgagc accggagatc gcgcagatat ctgtatctta tcggtgtgat
      841 catcatgatc gtttccagta cctattccgg tacgcggacc tcgaatatca tgttgcttgc
      901 ggggcttggg gtatatgtac tgttgatggc cgacaagaag tcgacgcgca tctttgccgg
      961 cgttgcgctt tttctgctga tagcggccat caagatgccc ttttatggaa gccctacgct
     1021 caaccggttt cgcagcactt tcgagggcaa gcatgatgaa tcctacctgg tgcgcgaggt
     1081 gaacaggaag cggatccagc cttatatcta ttctcacccg atcggcggag gtcttggtac
     1141 gacgaatgcc gcggggctag cctataatcc cggtcattac ctggccgatt ttcagacgga
     1201 tgacgggtat ctgaagacgg cgctcgagat cggctcgatt gggttgctga ttacctgtat
     1261 gctttatttt atggttttgc gaaccggggt gtctgagtat ttcaatacgt cgatcgagcg
     1321 gaacaaatcg gtgatatcgg cctgtcttgc gtgtatcttt tcctttatgc tggccgatct
     1381 ggcgcaggag ggtatcaacg agataaccaa tgtggcgatc tactatccgg cgatcgcgat
     1441 cctgttgagg gcgaattatg ttacctagag gtttcctttt ttcatttctg taacggcata
     1501 gtcgcatgcc cgggccgtca gggccatgta ggtgagcgat gggttttgtg tggcggagga
     1561 ggtcatgcag ctcccgtccg tgatgaagac gttctttacg tcatgcatct ggttgaagct
     1621 gttgaggacg gaggtcttcg ggtcgcggcc catgcgggcg gtgcccattt cgtggacggc
     1681 cgagccgccc ggctggaggt ggctataggc gttgatattt ttaaatcctg ttcgttccag
     1741 catttcccgg gtgctttcct ctacgtcctt ctgcatggcc cgttcgttct cgtggtattc
     1801 gaaatcgatc tttatcacgg gctgacccca tcgatccttt ttttcggtgt cgagggtcac
     1861 cttattggag taatagggca gggtctctcc ccagccgccc atccacaggt accaggggcc
     1921 tggctgtatt agtttgtccc ggaacccggg gccgaagcct tcgagtgtgg ccaattgttc
     1981 gacgccttcg agccgggcgc cgaatccctg gaggccatag ccgcggatga agtctttcct
     2041 ggcggtggaa gttccgagat tgcggaaccg ggggatgtag atgccggcgg gtctccggcc
     2101 ggagtagtat tgatcggcga agccttcgta ttcgccctgg gcgccgctgc cggtgaagtg
     2161 atccatgagg ttgtgtccca cctgttcgct ggaattcccg aggcctttag ggaaggcagg
     2221 ggatgtcgag ttcaggagga tcgcggcagt aggtatcgtc gatgcgttga gaaagatgat
     2281 cctggcgaaa tattccgtta tggttttggt gtgggcgttc atgaccctta ccccggaggc
     2341 gcggcgtttt tgttcgtcgt agaggatctc gacgacgatg gagtcgggca ggagggtcat
     2401 gtttccggtg agttctgcgg cgggcagggt ggctgcgttg ctgctgaagt agccgctgaa
     2461 agggcagccg cgggtgcaga gattgcggta ctggcagggg ccacggcctt tccagccctg
     2521 cgtgaggttg gctacgcggc ccatggtcac gatacggtcg gcatattttt cctttatctt
     2581 ttgtgcgaat tcggtctcga tgcaggtcat ctccattggc ggcaggaaga cgccgtcggg
     2641 caagtgggag agtccttcag gtttaccgct gatgcccacg aaggtttcga cgtagtcgta
     2701 ccagggggcg atatccttgt aacggatggg ccagtcgacg gctatcccgt cttttgcgtt
     2761 ggcttcgaag tcgaggtcgc tgaggcggta gcattgtctt ccccaggtga gggatctgcc
     2821 gcctacctgg taaccgcgga tccagttgaa gggtttctcc tggatatagg gatgctcccg
     2881 gtcgctgacg aagaactgtt tgttgctctc gctataggcc ccgctttgta tcgggtcttc
     2941 ttccttgttc tttcgggtgt tttcgaggtg gtgcggaaat tcccaggggg ccatgaaggc
     3001 cgtggtatag tcttttatat gccggacgtt gcgacctctt tccagtacca gggttttcag
     3061 gcctttttca cagagttctt ttgcggccca gccgccgctg atgcctgagc cgatgacgat
     3121 ggcgtcatag gaattggctt cgacgccatt tgtattgaga ttgggtattt gttttggatc
     3181 gcccatacgt gttcattttg ttgcccaagc tcgttgtccc tgcgtttttg ggatgcagcc
     3241 atgaaaacta ccggggatgg ctacgtaggc cagtcctttt cctgcgccca attccgatgt
     3301 gcagtagccc tgaacggtat attgccggag ttggtagaag aaggctttgc cgaggaattt
     3361 gttctcggct ttacccagga tgccgtccat ggggcggcct ttttcctcga aatgtttcag
     3421 gatagcggtt tgttgctccg gagtgcaggt ttcgtattct ttggtataat tagcccggct
     3481 atagtgggcg aggtctttta atccatcgat aaaaacgttg gcggtgtgga tcccggcgca
     3541 gtcttttacg atccggatga tatagtcgtg tacgcctgca tccgcggcgc cggggatcga
     3601 ggggccgggt ggcaggatag tctgtacgag ggcggccagg gtagcccggt gggtgtcgag
     3661 atagccgaag tcaggggatt tgttccattc gtaccatttg tacccagaaa aggcgccggc
     3721 gccgattaca ccggtgagca ggatgcggcc aatggctgtg cgtcttttca tgttagttga
     3781 tgaccgattc cagtcgtaca ccgatgatct gttcgatctc cagtttttgg aggtcggcct
     3841 gtaactgata attcaggagt ttggtcattt cggcggaata gttacgggag gcttcgctat
     3901 aggcttcgat cgagaccgtt ccgtcgttga accgcttctc cacctggagg aaagcggcgt
     3961 ggatatcatc ggccacctgt ctttcgagca atacgaggga gttggatacc cgccatcctt
     4021 tgtatttgga gaggacgtcg gtgcgtagtt ttagtgctgc ttcgatctgg ttatcctcgg
     4081 cgatctttac tccttccttg gcggctttta cctgaccgcc ttttgacagg atggtcccga
     4141 tggggatgga gacgccatag aagtatttgg gatatacata ggcgacattg ccaacggtcg
     4201 actggtgttt gaaggattgg tcattgaact gggaattgag ctgcagcagg tcgaaccagg
     4261 agttcttttc ctgttttacc ttgtaatgtg cgagggtgac ggtgcggata gcggcgtcgt
     4321 aggcgacgtt ttgaagagcc agtatgacca gtttctcctc cacgccggta tctttgatgg
     4381 ggagtgaccg gatggggcgt ttgcttaggg gtggggtgta gggcgagcgg ggtggctccg
     4441 actctgtctg tgcctgggtg gcgaaggcgg tgaggagcat aagagcgatc aacagataag
     4501 acccggcggg ccggatgctt ttttctctct tcatgagatg ggtgtttgac aagtaatata
     4561 cgggtttgcg tgaatcatta tttatggttc aggaaacgtt caccatttcc agatcaacct
     4621 tgttcagaat cgcgccactg aatttcccgt ttagttcttg gaagaattcc agggattcct
     4681 tgtcaatttg tttgatgatc tggtcggcgg agaagatacc gataacgcca tccacatatt
     4741 gtgccaattc acggctatcg gagaaatcat tcaggggcgg accttccagg aagatgaagt
     4801 cataatcgtg ggacaggctt ttgaggtgtt gaagcaggtg tttgcgtggg aggatctccg
     4861 atggggtata gtcaccccct tcggagccga tgatgaagac cgtatcgacg ccgatgtcct
     4921 tcgagaggtt tcttatctgc tcggccaggc tctcgtcggt gcgatcgttg gggacgatct
     4981 tctcgaggat gggtggcgct tccagtgcga cggtgaggtc gttattgcaa aagttggtat
     5041 cgatgatgag gatctttttc ttgctcatgc tcatgctgta ggcgagggcc tggatgatgg
     5101 tggtcttgcc ctggcctttt tttgtgctgg tgaacaggaa gatctttttg ccgctgatct
     5161 ctatttcgta tctcagttta cgaagggatt cgcggaagac gttgtgccgt tgtttatcct
     5221 tggggtcata ggaagtgtcg agggcggtga cgagttcgga gatggttttt tgtttcaggt
     5281 tcatgaaatt gatcatgctg atcagtttca gattgaccac ccgggcgaag agccggggtg
     5341 tcttaacgga ggagtcgaga taggcgagga tcaggatgat gaggatcgcc gtgattgccg
     5401 agccgacggc tgcgagtgca acgatcagta tttttttcga gggttcgggg acgatggcgg
     5461 gctggcccag caccacctgc cggaagttat tcagcgagga ggaggtgtag tcgatggcgt
     5521 cattgtagcg ctttttggca tcgagatagt ccttgttggc ctgttcggcg tccttcatca
     5581 gggtttcgac ggccacttcc ttcgatgcgt cctgggcggc ggtgcctctg aggatattga
     5641 tcttggcttg cagggagtcg atgtatttgg tgctttcctg gatatcgaaa tcgaggtcgc
//
LOCUS       DATGFG010000069         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1588310, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000069 DATGFG010000000
VERSION     DATGFG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588310"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VKQ52_01305"
     CDS             <1..751
                     /locus_tag="VKQ52_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ85838.1"
                     /translation="HDNDSGRILINATLAARLGIDPAKAPGLKLHSSYDSAKAFEIIG
                     VVKDFNFSSLHDAVSPFILIYDPGFPGNCTVTVSCNTSDYRSLLAQIAVIWRKTIADA
                     PFEYTFLDDTVRKQYEAEITLANIINVFTSLAILISALGLFGLAAFSAEQRSKEIGVR
                     KVLGATVTSVVRLLSTDFLKLVALALLISIPVSWWAMHKWLQGFAYQVPIRWWMFALS
                     GSLAVIIAFITVSFHTIRAANANPVRSLRSE"
     gene            complement(801..1610)
                     /locus_tag="VKQ52_01310"
     CDS             complement(801..1610)
                     /locus_tag="VKQ52_01310"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HLZ85839.1"
                     /translation="MTHFACPSFTKALVLLPMSLLASTTLVMAQYDSTDHRATEIYSP
                     VPPVVTPAATVGAPPSDAIVLFDGKNEDAWVRVADGAPADWIVSDGILTVNKKAGNIQ
                     TKQSFTNYQLHLEFRIPANITGEDQARGNSGLFLASTGKGDDGYELQILDNYNNKTYT
                     NGQVGSVYKQSIPLANPSRKPGEWSSYDVVWTAPVFNSDGTVASPAKVTAFLNGVLVQ
                     NGFELKGQTRYIGQPYYKMHGPSPIKLQAHGDKSEPISFRNIWVRPLEPLK"
     gene            1630..2535
                     /locus_tag="VKQ52_01315"
     CDS             1630..2535
                     /locus_tag="VKQ52_01315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ85840.1"
                     /translation="MNRQFRTNFLHSALACPLCGSTHTRARFFLPGSGVVYRCRECTV
                     EFISPQPDEIQLKKLYSESYYAAWGISGQQDNESTRAMKKATFRLRLALIRQYVPPGR
                     ILDIGCATGYFLEVAEEEGFKPFGVEFSAYAAARARLRFGAGAIFQGTLEQSFFPNNS
                     FMAITMSDLLEHVRSPSTTVTVAERLLNPTGVILIMTPDIGAPSRFLMGQRWTHYKPE
                     HLFYFNRRSITWLANRCGLQLEHFERSKKALNLSYLHTQFGVYRHWLLTPLVNMAWRL
                     APKNLRNRNFYLGIGEMAVILKKRA"
     gene            2592..>2995
                     /locus_tag="VKQ52_01320"
     CDS             2592..>2995
                     /locus_tag="VKQ52_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HLZ85841.1"
                     /translation="MQRLVMFSLSLLATAAFLSCTAQSPAPRVKVANGILEGVIEASG
                     VHSFKGIPFAAPPVGNLRWREPQPAVNWDGIRKADHFAAKAMQLPIFSDMQFRASGTS
                     EDCLYLNVWQPIDAGKKPLPVLVYFYGGGFVAG"
BASE COUNT          623 a          988 c          709 g          675 t
ORIGIN      
        1 ccacgacaac gactccggcc gcattctcat caatgccacc ctcgccgccc gtctcgggat
       61 cgatccggca aaagcccccg gcctcaagct ccattccagt tatgattcgg ccaaagcctt
      121 cgagatcatc ggcgtcgtaa aggacttcaa cttcagctcc ctgcacgatg ccgttagccc
      181 gttcatactc atttacgatc ccggtttccc cggcaactgc acagtcaccg tgagctgcaa
      241 tacatccgac taccgctcgc tgctggccca gatagccgtc atctggcgca agaccattgc
      301 cgatgccccc ttcgaataca cgttcctcga cgatacggtc cggaaacaat acgaggccga
      361 gatcaccctt gctaatatta tcaatgtctt tacgtcgctg gccatcctca tctcagccct
      421 cggcctcttc ggcctggccg ccttcagcgc agaacagcgc agcaaggaga tcggcgtccg
      481 taaagtcctc ggcgccaccg tcaccagcgt cgtccgcctc ctcagcaccg acttcctcaa
      541 actcgtcgcc ctcgccctgc tcatctccat ccctgtctcc tggtgggcca tgcacaaatg
      601 gctccagggc ttcgcctacc aggtcccgat ccgctggtgg atgttcgccc tctccggctc
      661 cctggccgtc atcatcgcat tcatcacggt cagcttccac accatccgcg ccgctaacgc
      721 caaccctgtc agaagtcttc gctcagaata aataaaaaaa gcccctgcgc aaacaggggc
      781 ttcactttct tatcatctct ctatttcaac ggctccagcg gccgcaccca gatattcctg
      841 aaactgatcg gctcgctctt atccccatgc gcctgcagct ttatcggcga aggcccatgc
      901 atcttgtaat acggctgccc gatatacctc gtttgcccct tcaactcaaa accgttctgc
      961 accaacactc cattgagaaa agccgtcacc ttcgccggcg aagccaccgt tccgtcggaa
     1021 ttgaacaccg gtgcggtcca gacgacgtcg tagctcgacc attcccccgg cttgcggctg
     1081 ggattggcca gcgggatgct ctgcttatac acgctaccca cttgcccgtt ggtataggtc
     1141 ttattgttgt agttatccag tatctgcagc tcgtacccat cgtcgccctt acccgtcgac
     1201 gcaaggaaca gaccgctgtt accccttgcc tgatcttccc ccgtaatatt ggccgggata
     1261 cggaattcca ggtgcagctg gtaattcgta aagctctgtt tggtttggat atttcccgct
     1321 ttcttgttga ccgtcagaat tccgtctgaa acgatccagt ccgccggcgc accatcggca
     1381 acccttaccc acgcgtcttc gttcttacca tcgaagagga cgatcgcgtc ggaaggtggg
     1441 gcgcccaccg ttgcagccgg cgtcaccacc ggcggaacgg ggctgtaaat ttcggtggcc
     1501 cggtgatccg tggaatcgta ctgggccatc accagggtag tgctcgccaa caggctcatt
     1561 ggtaaaagaa caagcgcttt ggtaaaggaa ggacaagcaa aatgagtcat aaatcaaatg
     1621 ttgttaagaa tgaaccgaca atttaggaca aattttttac attctgccct ggcctgtccg
     1681 ctttgcggtt cgacccatac gagggcccgt ttcttccttc ccgggagcgg cgtggtctat
     1741 cgttgtcgtg agtgcaccgt cgagttcatc tcgccgcagc ccgacgaaat tcagctaaaa
     1801 aaattgtatt ccgaaagcta ttatgccgcc tggggcatca gcggacaaca ggacaatgaa
     1861 agcacccgag cgatgaaaaa ggccaccttt cgccttcgcc tcgccctgat tcgacaatat
     1921 gtaccgccgg gtcgtatcct cgacatcgga tgcgccacgg gctacttcct ggaagtggca
     1981 gaagaagagg gtttcaagcc cttcggggtc gaattctctg cttatgccgc cgccagggcc
     2041 cgcctccgtt ttggagcggg ggcgatcttc cagggcacgc tggaacagtc cttctttccc
     2101 aacaactctt tcatggcgat aaccatgtcc gatcttctcg agcacgtccg ctctccctcc
     2161 acaaccgtta cggttgctga aaggttgcta aaccccacag gggtcatcct cattatgacc
     2221 ccggacatcg gtgccccgtc gcggttcctg atgggccagc gctggaccca ttacaaaccc
     2281 gaacatcttt tctacttcaa tcgccgtagc attacctggc tggccaaccg ctgtggtctc
     2341 cagttggagc acttcgaacg atcgaaaaaa gccttgaacc tcagctatct tcacacgcag
     2401 ttcggtgtct atcgccactg gcttcttact cccttggtta acatggcctg gcgcctggcg
     2461 cccaaaaacc tccgcaaccg gaatttctac ctcggcatcg gggagatggc ggtcatcttg
     2521 aaaaaaaggg cctgaccctc cagcgcccgt caaaaatgct ttaaattagg ggacatcaaa
     2581 ccctcctcca catgcaacgg cttgtcatgt tctctctctc tctgctggct acggcggcct
     2641 tcctctcctg caccgcacaa tcccccgctc cccgcgtcaa ggtcgccaac ggaatactcg
     2701 aaggggttat cgaagcaagc ggggtacact cgttcaaagg catccccttc gccgccccgc
     2761 ctgttggcaa ccttaggtgg cgtgaacccc agcccgccgt caactgggat ggcatacgca
     2821 aggccgacca cttcgctgca aaagccatgc aactccccat cttcagcgac atgcaattcc
     2881 gtgcctcggg caccagcgag gactgtctct atctgaacgt ctggcaaccc atcgacgccg
     2941 ggaagaaacc attgcccgta ctcgtctact tctatggggg tggctttgtc gccgg
//
LOCUS       DATGFG010000070         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1701670, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000070 DATGFG010000000
VERSION     DATGFG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701670"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..449)
                     /locus_tag="VKQ52_01325"
     CDS             complement(219..449)
                     /locus_tag="VKQ52_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HLZ85842.1"
                     /translation="MASNPTTPRALFILAATTCLLTACTTVKDYQKNRLNDSEMSLTN
                     RKVEKNEANFQSYREGSSGANGGKTGGGCGCN"
     gene            complement(455..1381)
                     /locus_tag="VKQ52_01330"
     CDS             complement(455..1381)
                     /locus_tag="VKQ52_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HLZ85843.1"
                     /translation="MRTVYKQACKLMGCCFETSAVTDSATQAAEAILAAEEEMRRIER
                     LLTTYDENSETALINRNAGEEPVRVSAETFRIIERSIRISELTSGAFDISYGSVDKSL
                     WNFDTSLQQLPDAATAKKMVRLINYRNILLDKENTTVLLKEKGMRIGFGGIGKGYAAE
                     RAKAVMREMGIDSGIVNASGDLAVWGLQPNGEPWRIGIVHPDAPDQAFCHLQVTNMAL
                     ATSGNYEKFAIIDGKKYSHTINPRTGLPVSGIKSVTIITPNAEIADAMATPVMIMGIA
                     AGIDMINQMKDIEAVLIDDDNQVFTSKNIHLS"
     gene            complement(1385..1825)
                     /locus_tag="VKQ52_01335"
     CDS             complement(1385..1825)
                     /locus_tag="VKQ52_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLZ85844.1"
                     /translation="MLKILLSILLLSASWTTEWHHDLREAENVAKNEHRLVLLNFSGS
                     DWCGPCIRMHKEVFESNDFRYLADSTLVLVNADFPRMSRNQLPAKQQQLNNAMADQYN
                     PKGKFPLTVLLTADGKVLRAWDGFPDGGAAAFTEQLHSVIAGNR"
     gene            complement(1977..2417)
                     /locus_tag="VKQ52_01340"
     CDS             complement(1977..2417)
                     /locus_tag="VKQ52_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLZ85845.1"
                     /translation="MSTRIKIAKADPAAYKAMMALDKYLETTRLSPTHKQLIKIRASQ
                     LNGCSYCIDKHTKEARQIGDTEQRIYALSAWRETPFFTPEERAVLAITEEITFIGNRV
                     SDKTYDEAAALFDEEYLAQLMMAAIIINAWNRIGVATAMSPQLP"
     gene            complement(2494..3081)
                     /locus_tag="VKQ52_01345"
     CDS             complement(2494..3081)
                     /locus_tag="VKQ52_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ85846.1"
                     /translation="MIVSDPYAPLFAHMRKFVALDEEEEAILAAGLRHKKIKKKELLL
                     KEAQVCNANYFVLRGCLRMYFIREDGVEQIIQFAIENWWITDFQSLDWHQPSRFNIQA
                     IEPAEIAILDRTVTPTLCARLPQLDHYLRLVVQRAFAAAQQKLFFIHTFSGEERYHHF
                     SNTFPQFVQRIPQYMVASYLGFTPEFVSKIKTRRK"
     gene            complement(3086..3664)
                     /locus_tag="VKQ52_01350"
     CDS             complement(3086..3664)
                     /locus_tag="VKQ52_01350"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ85847.1"
                     /translation="MPRTKSFDEEETLDKAKQVFWKKGYNGTPPQDILDGTGLSRSSL
                     YDTYGDKHTLFIRTLQRYRQCETASAIQYLDEAPYPGQAIRNIFNAAHDAFTTAMPRM
                     GCLMVNTLNEVAPHDTEVEAIVNENRQALEDAYARAIKRGQQQGEIATEQQPRALARF
                     LLNSLWGLTTNLKLGLDKKAADDIVIITLSVL"
     gene            3838..4602
                     /locus_tag="VKQ52_01355"
     CDS             3838..4602
                     /locus_tag="VKQ52_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ85848.1"
                     /translation="MANRTKIALVTGGSRGLGKNMALQLAGKGVDIILTYNSNKAEAE
                     KVVAQIRAMGRNAEAYQLDTTNIKSFDPFLKEVSAHLKKYTGSGNFDFLINNAGTAAH
                     HSIAETPEDVFDQLYHIHLKGVFFLTQKALPFLNDNGRVINISTGLARFTYPGFAAYA
                     SMKGAIEVFTRYLAKELGSRGITVNTIAPGLTETDFTRASLPTTEEGRKQVGSIFALG
                     RIGQPDDIGAAVTLICSEESRWITAQRIEVSGGQQL"
     gene            complement(4612..>4935)
                     /locus_tag="VKQ52_01360"
     CDS             complement(4612..>4935)
                     /locus_tag="VKQ52_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HLZ85849.1"
                     /translation="KNQMVIAITLLRQRNSLELQKEVTKTTNELLIKNSQLLKEGTIE
                     IAKESERGIVEIETLKKVNTDLIGTIEETIKIQADGSAKRRQAEGELVRLENDLKDKL
                     LSLKS"
BASE COUNT          926 a         1147 c         1597 g         1265 t
ORIGIN      
        1 tcccaccctg gatggcggag tgatctccgg tttgggtgta gtagctgctg acgaggttga
       61 tctcgtcgag ttttaatttc cgcggtttga atacagaggt gtcggccggc gttgtttggg
      121 aaaaggcatg gaggagcatc agatagaggc cgatgacggt aagacagatc tttctcatgt
      181 gttgtggatg ggtggtgtgg atgggttttc ttacgctctt agttacagcc gcagccgccg
      241 ccggttttgc cgccgttggc gccggaagag ccttcgcgat aggattggaa gttggcttcg
      301 ttcttttcga ccttgcggtt ggtgagggac atctctgagt cgttgagcct gtttttctgg
      361 tagtccttta ccgtcgtgca ggcggtgagg aggcaggtgg tggctgcgag gatgaagagg
      421 gcccgggggg tagttgggtt tgatgccatg ttttttagct taggtggata tttttggatg
      481 tgaatacctg gttgtcatcg tcgatgagca cggcttcgat gtccttcatc tggttgatca
      541 tatcgatgcc ggcggcgatg cccatgatca tcaccggtgt agccatggca tcggcgatct
      601 ccgcgtttgg cgtgataatg gtcacgcttt ttattccgct gaccggcagg ccggttcgtg
      661 gattgatggt gtgggaatat ttcttcccgt cgatgatcgc gaatttttca tagttgccgg
      721 aggtggccag ggccatattg gtcacctgca ggtggcagaa ggcctggtcg ggcgcatccg
      781 gatgaacgat gccgatgcgc cagggttcgc cgtttggctg gaggccccat acggccagat
      841 cgccggaggc gttgacgata ccgctgtcga tacccatctc ccgcatgacg gcttttgccc
      901 tctcggcggc gtagcccttc cctatcccgc cgaatccgat gcgcattcct ttttctttta
      961 gcaggaccgt cgtgttttct ttatccagca ggatattcct gtagttgata aggcgtacca
     1021 tcttcttcgc ggtggccgca tccggcagtt gttgcagcga ggtatcgaaa ttccacaggc
     1081 ttttatccac cgatccatag gaaatatcga atgcgccgct ggtcagttcg gagatacgga
     1141 tggagcgttc gatgatcctg aaggtctccg cgctgacgcg gacgggttct tcgcctgcgt
     1201 tgcggttgat cagggctgtt tcgctgttct cgtcataggt ggtgagcaat cgttcgatgc
     1261 gccgcatctc ttcctccgcg gcgaggatgg cttcggctgc ctgcgtggcg gaatccgtca
     1321 ccgcgctggt ctcgaagcag catcccatga gtttacaggc ttgtttatat actgtccgca
     1381 tgccttatcg gttgccggca atgacggaat gcaattgttc ggtaaaggct gccgctccgc
     1441 catcgggaaa accgtcccag gccctgagca cttttccgtc ggccgtcagg agtaccgtga
     1501 gcgggaattt tcctttcggg ttgtactgat cggccatggc attgttcaat tgttgctgtt
     1561 ttgccgggag ctggttcctg ctcattcgcg gaaagtccgc gttaaccagg acgagggtcg
     1621 agtcggcgag atagcgaaag tcattgcttt cgaagacctc tttatgcatc cggatacagg
     1681 gcccgcacca gtcggacccg gaaaagttga ggagtacgag gcggtgttcg tttttagcga
     1741 cattctcggc ttcccggaga tcgtgatgcc attcagtggt ccaggatgcg gagaggagca
     1801 gaatggataa gaggatttta agcattattg cgttgaattt tgaagttttc cctgttcttt
     1861 agggatacgc aaataaatta aaaagagttg ctatgccaag cggggaattt ttctttaaca
     1921 atgttgtaac gttaaaaagg gtgccgcccg gggaggtcat ggggccgttt gccggctcag
     1981 ggcagttggg ggctcatggc ggtggcgacg ccgatgcggt tccaggcatt gatgatgatg
     2041 gcggccatca tgagctgggc gaggtattct tcgtcgaaga gggcggcggc ttcgtcgtag
     2101 gttttgtcgg agacccggtt gccgatgaag gtgatctctt cggtgatggc caggacggcg
     2161 cgttcttcgg gggtgaagaa gggggtttcg cgccaggcgc tgagggcata gatccgttgt
     2221 tcggtgtccc cgatctggcg ggcttctttt gtatgtttgt cgatgcagta ggaacagccg
     2281 ttgagctggg aggcccggat ctttatgagc tgtttgtgtg tgggggagag acgggtcgtt
     2341 tcgaggtatt tgtcgagggc catcattgct ttgtaggcgg cggggtcggc ttttgcgatc
     2401 ttgatacgtg ttgacatgcg tttgtgtttt tgacaggaca aaattgggaa ggggccgggc
     2461 cgggaaaaat aatcatgatt aagaaaacgg gggttatttg cggcgggttt tgatcttgct
     2521 gacgaattcg ggggtgaagc cgaggtagga ggcgaccatg tactggggga tgcgctggac
     2581 gaattggggg aaggtgttgc tgaaatgatg atagcgttct tcgccggaga aggtgtggat
     2641 gaagaagagc ttttgctggg cggcggcgaa ggcgcgctgg acgacgaggc ggaggtagtg
     2701 gtcgagctgg ggcagacggg cgcagagcgt gggtgtgaca gtgcggtcga ggatcgcgat
     2761 ctcggcgggt tcgatggcct ggatgttgaa gcgggagggt tggtgccagt cgaggctttg
     2821 gaagtcggtg atccaccagt tctcgatggc gaactggatg atctgttcga caccgtcctc
     2881 gcggatgaag tacatgcgca ggcagccccg cagcacaaaa tagttggcgt tacagacctg
     2941 tgcttccttt agcaggagtt cctttttctt tatcttttta tgccgcagac ccgcggcgag
     3001 aatagcctct tcctcctcgt cgagggcgac gaatttccgc atgtgggcaa agagcggcgc
     3061 gtagggatcc gatacgatca cgtggttata atacagataa agttattatt acaatgtcgt
     3121 cagcggcctt tttgtcgagg ccgagcttca ggttggtggt caggccccac aggctgttca
     3181 acaggaaacg ggcaagggcg cggggttgtt gttccgttgc tatctctccc tgctgctggc
     3241 cacgttttat cgcacgggca taggcgtctt ccagcgcctg gcggttctca ttgacgatcg
     3301 cctccacttc ggtgtcatgg ggcgccactt cattcagcgt attgaccatg agacaaccca
     3361 tgcgtggcat ggcggtggtg aatgcatcgt gggcggcgtt gaagatattg cgaatggcct
     3421 gccccggata gggcgcttcg tccaggtatt ggatggcgga ggcggtctca cattgccggt
     3481 aacgttgcag ggtccttatg aacagggtgt gtttgtcgcc ataggtgtcg tagaggctgc
     3541 tccggctgag accggtgccg tcgagaatgt cctggggagg ggttccgtta tagccttttt
     3601 tccagaagac ctgtttggct ttgtccagcg tttcctcttc gtcgaatgat ttggtgcgcg
     3661 gcatttgcgg tttttgatgg ctacaaagct acaaattttg ggccgaagga tggcgggcgg
     3721 cggggaatgg cggagatggc cgggggcacg ggcaggctcg gggcaggcga aaatactgaa
     3781 gtggtcacca tgccggtgtc ggaaaagccg gcgacttttg tagcctaaat caattttatg
     3841 gcaaacagaa caaagatcgc attggtcacc ggtggcagca ggggcctggg aaagaatatg
     3901 gcgctgcagc ttgccgggaa aggagtggat attatcctga cctataacag taacaaggct
     3961 gaagcggaga aagtagtcgc gcagatcagg gcgatggggc gcaatgccga agcctatcaa
     4021 ttagatacga cgaatataaa atccttcgac ccctttctaa aagaagtatc ggcccatctg
     4081 aaaaagtata ccggaagcgg caatttcgac ttcctgatca ataacgccgg gacggccgcc
     4141 catcattcca tcgccgagac gccggaggat gtgttcgatc agctttacca tatccacctg
     4201 aaaggggtgt tcttcctcac ccagaaggcg ctgcccttcc tcaatgacaa cgggcgggtg
     4261 atcaatattt cgacgggatt ggcccggttt acttatcccg gttttgcggc ctatgcctcc
     4321 atgaagggag ctatcgaggt gtttacgcgt tatctcgcca aggagcttgg gtcgaggggg
     4381 ataacggtta atacgatcgc gcccgggctg acggagacgg attttaccag ggcgtcgctg
     4441 ccgaccaccg aggaaggccg gaagcaggta gggtccattt ttgcgcttgg ccggatcggg
     4501 cagcccgatg atatcggcgc cgcggtgacg ctgatctgca gcgaagagtc ccggtggatc
     4561 accgcccagc ggatcgaggt gtcgggcggg cagcagttgt agaccacgcc cttagctctt
     4621 taatgagagc agcttgtcct tgaggtcgtt ttcgagacgg acgagttcgc cttccgcctg
     4681 acgacgcttc gcgctgccgt cggcctggat cttgatggtc tcctcgatgg tgccgatgag
     4741 atcggtattc acctttttga gcgtttcgat ctcgacgata ccgcgttcgg attcctttgc
     4801 gatctcaatg gttccttcct tcaggagctg gctgttcttt atcagcagtt cgttcgtggt
     4861 ctttgtgacc tccttttgca gttccagcga gttgcgctgg cgcaggaggg ttatagcaat
     4921 gaccatctga ttttt
//
LOCUS       DATGFG010000071         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_900148, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000071 DATGFG010000000
VERSION     DATGFG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900148"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VKQ52_01365"
     CDS             complement(<1..1041)
                     /locus_tag="VKQ52_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85850.1"
                     /translation="MISLHLAIKNHLLFGRDYLFTYGPLGVLRCRYPIVVSKYVYLAS
                     DLYFLFILFTAFRLLIRRHFQAGAIVFLSVCVLICQYMNIDKWYFFLLLFFLAAYLSE
                     GGKLVYLLHAAVLSVICLYIKVNSGLVNIVFVLAFLHYALFTRKIRWAGYGLFLVGYG
                     ITLWLSAWLLHVDLPGYIEGSFQIMKDYQDAMYFPLWGRIGKIVGIGAGIILLLLFGC
                     YGIVAYHYWKVKRITTDTLDILFIYGMVALALFIWFKNAFVRGDSEHLFHFFQMAGPL
                     VLLLYLYAPDGPFRKRIQAGYWLILAVTVVVVNLLPTDLYPNSFRRLVDLSLAPEKVR
                     EVRTYFKGLAGYD"
     gene            complement(1309..2586)
                     /locus_tag="VKQ52_01370"
     CDS             complement(1309..2586)
                     /locus_tag="VKQ52_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HLZ85851.1"
                     /translation="MHRITLLLFVLCLGFVAPAQDLMGSLKNCASTVRSIEVGDTDFT
                     DLDGLRAAIGGARVVLLGEQTHGEGSTFQAKTRVIKFLHEKMGFDVLAFESGFYDCAR
                     IWENVEEGGTLSQEVIGSLFYMYATSVQMQPLFDYIQSNLHAAHPLTLTGFESQHTGV
                     KAKTQLFVDFERFLKKQLPDAVDENWELFRRISIATFGSRDYRPSIEEKARFFRKLEQ
                     LQRVLEDGSKVADSLTSSMGFWYQVVNSIESQTTRYWQMVTGNEVSVRDLQMAKNLIW
                     LAEKAYPGKKIIVWAHNVHISKGLSTLTIGNQQPQKGDNAFVPMGATIHRAFGNRAYV
                     IGFSGSEGSYMNYENDQIVTVAPRPAGTIEGVLAGAGYTYAFVDYRRASGPLRQPQQA
                     TLADFGNVRGIWPEVFDGMFFIARVWPVERMGK"
     gene            complement(2660..>2944)
                     /locus_tag="VKQ52_01375"
     CDS             complement(2660..>2944)
                     /locus_tag="VKQ52_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85852.1"
                     /translation="QRRDCLCGLLDAHLKDKVSFRRPSGGMAVWACFARKYPLKTVAA
                     EALRQGLFMYNGQQYNTHNTDYNALRLGFASLTEEELGEAMEILSGILSP"
BASE COUNT          813 a          906 c          652 g          573 t
ORIGIN      
        1 gtcatagccc gcaagcccct tgaaataggt gcgtacctcc cgcactttct ccggcgccag
       61 cgatagatcc accagccggc ggaagctgtt tggatacagg tcggtcggca acaggttcac
      121 tacaacaacg gtcaccgcaa ggataagcca gtagccggcc tggatccgct tccggaaagg
      181 cccatccggc gcatacaggt aaagcagaag aaccagcgga ccggccatct gaaaaaaatg
      241 gaacaggtgc tcggagtccc ccctgacaaa ggcgttctta aaccagatga acaaggccaa
      301 tgccaccatg ccatagatga acaggatatc cagcgtgtcg gtcgttatcc gctttacctt
      361 ccaataatga tacgccacga tcccataaca cccgaacagc aacagcagga tgatcccggc
      421 cccgatccct acgatcttcc caatcctccc ccatagcgga aaatacatcg catcctggta
      481 gtccttcatg atctggaaac ttccctcgat atagcccgga agatcgacat gcagtaacca
      541 ggcgcttagc cacaacgtga tcccataacc caccaggaac agcccatagc cggcccatcg
      601 tattttccgg gtaaaaagcg catagtgcag gaaagccagg acaaacacga tatttaccaa
      661 acccgaattc accttgatat aaagacagat cacggacaat acagcggcat gaaggagata
      721 aaccagcttg cccccctcac taagataggc ggcaagaaaa aaaagcagca ggaaaaaata
      781 ccacttgtcg atattcatgt actggcagat caggacgcac accgacagga acacgatcgc
      841 gccggcctgg aaatgccgcc tgatcaaaag ccggaaggcc gtgaacagga tgaaaagaaa
      901 ataaagatcc gaagccaggt aaacatattt ggatacgaca atgggatatc ggcatcgaag
      961 gaccccgagc ggaccatagg taaatagata atcccgtccg aaaagcaaat gattcttgat
     1021 cgccaggtga agagagatca tccaggatgg atcgagcccc gtttcgggta atgaaaaaaa
     1081 cgccggtggg aaaagtagaa aaataaaaat gataggaatg aaccaggcaa aaaaacgcct
     1141 ctttccgggg gataaagtct ggaacatgag atggtctgtt atgttcagct taaatataat
     1201 caattaaagc tgaaaaaagc atgccaactc cctccccgcc gcatatgcgc agtatcgcta
     1261 atatataaca cgcatgcatc tcaaaacctg agcgatttct ttgggaactt acttccccat
     1321 gcgctcaacc ggccacaccc tggcaataaa gaacatacca tcaaaaacct ccggccagat
     1381 cccccgcaca ttgccaaaat ccgccagcgt ggcctgctgc ggctgccgta atggccccga
     1441 cgcccgccgg taatccacaa aggcataggt atatcccgcc cccgccaaca ccccctcgat
     1501 cgttcccgcc ggccgcggcg ccacggtgac tatctggtca ttctcataat tcatatagct
     1561 gccttccgac cccgaaaatc cgataacata cgcccgattc ccaaaagccc gatggatcgt
     1621 ggcccccatc ggaacaaagg cattgtctcc cttctgcggc tgttggttgc cgatagtaag
     1681 cgtcgaaagc cccttggaga tatgtacatt atgcgcccac acaatgatct tttttccggg
     1741 ataagccttt tcggccagcc agatcaggtt cttcgccatc tgcagatccc gtacactcac
     1801 ttcgttgccg gtcaccatct gccagtacct tgtcgtctgc gactcgatac tgttcaccac
     1861 ctggtaccag aaccccatgc tgctcgtcag ggaatccgcc accttcgacc catcctccag
     1921 cacccgctgc aattgctcaa gtttccggaa gaaccgcgcc ttctcctcta tcgatggacg
     1981 ataatcccgc gaaccaaagg tcgcaatgct aatacggcga aacagctccc agttctcgtc
     2041 aacagcgtcg ggtaactgct tttttaaaaa ccgctcaaaa tccacgaata gctgcgtttt
     2101 agcctttaca cccgtatgct ggctctcaaa accggtgagc gtcagcgggt gggccgcatg
     2161 caggttcgac tggatatagt cgaacaaagg ctgcatctgg acactcgtag cgtacatata
     2221 gaaaagactg ccgatcacct cctgcgacaa cgttcctcct tcctcgacat tctcccagat
     2281 ccttgcacag tcatagaacc cgctctcgaa agcaagcaca tcgaaaccca tcttctcatg
     2341 caggaatttt atcacccggg tcttcgcctg gaaggtcgac ccttccccat gcgtctgctc
     2401 ccccagcagc acgacccgcg ccccgccgat cgccgcccgc agaccatcca gatcggtaaa
     2461 gtccgtatcc cccacctcga tcgaccgcac cgtagaagcg caatttttaa ggctgcccat
     2521 aagatcctgg gccggcgcca caaaacccag gcaaagaaca aatagtaaaa gtgtgatccg
     2581 atgcatacga ttgatgattt cagcagcgaa acaagcaact ccgccggagt ctgccaacac
     2641 ttcgtttgct aatatttagt taaggtgaaa ggatcccgga caatatctcc atcgcctcgc
     2701 ccaattcctc ctccgtcagg gaagcaaatc ccagccgcag ggcattataa tccgtattat
     2761 gggtattgta ctgctggccg ttatacataa acaacccctg ccgcaacgcc tcggccgcca
     2821 ccgtcttcag cgggtatttc cgggcgaaac aggcccacac cgccatcccc ccggaaggcc
     2881 gccggaaaga gaccttatcc ttcagatgcg catccagcaa cccgcacaaa cagtcccgtc
     2941 gctg
//
LOCUS       DATGFG010000072         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1125446, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000072 DATGFG010000000
VERSION     DATGFG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125446"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="VKQ52_01380"
     CDS             complement(<1..1783)
                     /locus_tag="VKQ52_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLZ85853.1"
                     /translation="MNSHLQEKDEMIPASHQQQGIWFHAHINSASYWNFIQKRAYKGF
                     LHIDAIQNALGIIISRHSALRTNFRLYDEKLYQVVNAHPSMSTIFHHVRFDRGDDVFM
                     AERVAEESIREEGYDPDLEHDPLIRFKVLEFGDTCFFLLTIHHIVTDAVSMQLFWDEL
                     IHYYNATVRGVQPERPLPVISYPAYSSEQEAGKRTAAYMQQEKYWNDLLSVDLPLLNL
                     GFYGSTAPAQLFHHETGIPPALTNAIRTLSLKKKILYSSVFQTAWFIFLHKYSNNTTL
                     AVGNVVNGRGFGKRNYKDVIGLFATRIVNIQKIAEEATFTDLLQSVNSGLLAGFQNSD
                     IPYESLVRHLNRSGSSGGASLFQASFNMIKESKEGSSFIGLEEYPIPQAGNGQIGDNQ
                     YEIGLSIRDTGSEVLLRLDVKCEESLRSLPGLLLRGYLQVLNDCVHAPATKISGIYLP
                     AGDQPGPVNEVNDPAAGEGRTVVDLFMEQVTRTPQAVALVSGQEAVTYRRLDERSNRI
                     AHRLGRLGVREGTPVGICLERGPEMIAGILGILKAAGAYVPIDVQYPVDRIGHILQDT
                     AAPVVLTSRNGNRALPPGYTGAVVRLDE"
     gene            complement(1796..>2366)
                     /locus_tag="VKQ52_01385"
     CDS             complement(1796..>2366)
                     /locus_tag="VKQ52_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85854.1"
                     /translation="HSNEIALPSEREEIAFMGRAMEMVMAGDYQLWSTFAFTRNGCQS
                     RYIEYTWRNQDMIAYGASSFGKIGNLNYQNLNNIQQYSDKVRNNAIPVYRTYQMSYKD
                     MIVKELLLCAVRLFSYKKAEFVDKFGFDYFDLIPDLIGDLVRKGYITADTEELVLTRQ
                     GILFADFVSKAIATAVKNALAGDTIGFTY"
BASE COUNT          528 a          647 c          605 g          586 t
ORIGIN      
        1 cttcgtccag ccggaccacc gccccggtgt agccgggcgg cagggccctg ttgccgttgc
       61 ggctggtgag caccaccggg gcggcggtat cctgcaggat atgcccgatg cgatcgacag
      121 ggtattggac atcgatcggc acataggcgc cggcggcctt gaggatgccg agaatgccgg
      181 caatcatctc cggcccccgt tcgagacaga tgcccaccgg cgttccctcc cttacgccga
      241 gtcgtccgag ccgatgggcg atccggttgg agcgttcatc caggcgacgg taggtgactg
      301 cctcctgccc ggaaaccagg gccaccgcct gcggcgtccg ggtgacctgt tccatgaata
      361 gatccaccac cgtcctgccc tctcccgctg caggatcatt tacctcgttc accgggcccg
      421 gctgatcgcc ggccggcaga tatatcccgc ttattttcgt cgcaggggca tggacacagt
      481 cgttcaaaac ctgtaagtac ccccgtaaca aaagaccagg caacgaccgc agggattcct
      541 cacactttac gtcgaggcgt aacaacacct cgcttcccgt atccctgatg gatagcccga
      601 tctcgtattg attatcgcca atctggccgt taccggcctg cgggatcggg tattcttcca
      661 ggccgataaa ggaactgcct tccttcgact cttttatcat gttgaatgag gcctggaaca
      721 acgaggctcc tcccgatgag ccgctcctgt tgagatgtct gaccagggat tcataaggga
      781 tatcgctgtt ttgaaaaccg gcaagcagcc ccgaattgac gctttgcaac aggtcggtga
      841 aggtagcttc ttccgctatt ttctgtatat tgacgatacg ggtggcaaaa agaccgatca
      901 catccttata gtttctcttt ccaaatcccc ggccattcac cacattacct acggccaggg
      961 tggtattatt cgaatatttg tgtaaaaaga tgaaccaggc cgtttgaaac acggaggaat
     1021 agaggatttt cttttttagg gataaggtcc ttatggcatt tgtcagtgcc ggggggatgc
     1081 cggtctcgtg atgaaaaagc tgcgcggggg ccgtgctccc gtaaaagccg agattcaaca
     1141 gcggcagatc cacggaaagc aggtcgttcc agtacttctc ctgctgcata taggcagccg
     1201 ttcttttgcc ggcttcctgt tcggaggagt atgccggata gctgatgacc ggcagcggac
     1261 gttccggctg caccccgcgc acggtcgcat tataataatg gatcagttca tcccagaaca
     1321 actgcatcga cacggcatcc gttacgatat gatgtatggt aaggagaaag aaacaggtat
     1381 cgccgaattc caataccttg aaccggatca acgggtcatg ctcaaggtcg ggatcgtatc
     1441 cttcttcccg tatcgattct tccgcaaccc tttccgccat gaacacatcg tctcccctgt
     1501 caaatcttac gtgatgaaaa atggtactca tcgaaggatg ggcatttacc acctggtata
     1561 acttttcatc atagagcctg aaattcgttc gcaaggcgga atgcctgctt atgatgatgc
     1621 caagggcgtt ttgtatggca tcgatatgca aaaacccctt gtacgctctt ttctgaataa
     1681 agttccaata cgatgcactg tttatatgcg catgaaacca tatgccctgt tgttgatggg
     1741 atgccgggat catttcatcc ttttcctgca gatgactatt catgtgtctg gacaattaat
     1801 acgtgaaccc gatggtgtcc ccggcaagag cattctttac agcggtggcg atggctttcg
     1861 atacaaaatc tgcgaacagg atgccctgcc tggtgagcac cagttcctcc gtgtccgcgg
     1921 tgatatagcc cttgcggaca aggtccccga taaggtctgg tatcaggtcg aaatagtcga
     1981 acccgaattt gtccacgaac tcggcttttt tgtaggagaa caggcgaaca gcgcagagga
     2041 gcagctcttt tacgatcatg tccttatagg acatctggta agtccggtac accggtatcg
     2101 cattattcct taccttgtcg gaatactgtt ggatgttatt caggttctga taattcagat
     2161 ttcctatttt cccgaaggaa gaagctccgt aagctatcat gtcctggttt ctccaggtgt
     2221 attcgatata ccggctctga caaccgttcc tggtaaaggc aaaggtgctc cataactgat
     2281 aatccccggc catcaccatt tccatggccc tgcccataaa agcgatctcc tcccgctcgg
     2341 atggcagggc gatctcgttg ctgtgc
//
LOCUS       DATGFG010000073         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_785465, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000073 DATGFG010000000
VERSION     DATGFG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785465"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..794)
                     /locus_tag="VKQ52_01390"
     CDS             complement(12..794)
                     /locus_tag="VKQ52_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85855.1"
                     /translation="MKSVFFILTAAAILAACTKTITPALNNAGSQVYIQGAVSDTAGP
                     YFVSIVRSVGFYDSSIYPGIAGAAVTITDSTTGQQDALTETSTGLYVTHTLTQGIPGH
                     TYLLNVRLNGTTYTASSTMPQPVPLDSVSFDFSDTTRIEAVANYQDPVHIINFYKYTM
                     TINGTVDKRFLTFEDRLSDGRYIHDRIDADTGEVKRYDVVRLSLVGIDSNVYTYLHEA
                     EQIAYNNAGLVSPANPQSNITGGCLGYFSAQTVSSKTGIARQ"
     gene            complement(800..>2509)
                     /locus_tag="VKQ52_01395"
     CDS             complement(800..>2509)
                     /locus_tag="VKQ52_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ85856.1"
                     /translation="DVNLYKGGMPASYGGRLASVEDIRMKDGNDQRLSGSGGIGLIAS
                     RLNLEGPIVKDKSSFIVSARRSYVDLFTPLASDTNVKHSKLYFYDVNLKANYSFDSKD
                     RIFLSAYFGKDVLSQRYKNGVDWGNQTATLRWNHVFSTRLFSNASLIFSNYNYNAQFA
                     SLGSNTYVRSGITDYHFKEDLNYYVSPHSKLDIGLDMTYHITQPGQSTTSGNSKFNDI
                     TLEKKYAMENAIYASNEWSPNTRFKLVYGVRLTQLAVLGPGNAYTYDQQGNILTKKYY
                     SGGKEIITYWNPEPRIAATYQLNNTSSVKLAYDRTVQNIHLLNNATTASPTNVYLPTS
                     NIVKPEISDQVSAGYYSYFHHRMYEFSTEVYYKEMQNQIDYANGVNLVGNNNIEAELL
                     FGKGRGYGWENYIHKTSGRLTGWVSYTLSRTERQIAGVNNGSWYPAVQDQTHSLSVVG
                     IYKYNRKWTFSADFVYNTGNPTTWPSGKYPVDGAPVYYYTARNDYRLPAYQRLDLGAT
                     LQLKKTPKFESDLNFSIYNAYGYRNPYTITFQQDPTNKLLTQVQQTTLFTFVPSITYN
                     FKF"
BASE COUNT          522 a          613 c          730 g          644 t
ORIGIN      
        1 gaaatggttg attattgcct tgcgatcccc gttttgctgc ttacagtctg ggcgctgaaa
       61 tagcccagac atccgccggt gatgttggac tggggattgg ccggggaaac caggcccgca
      121 ttgttgtagg cgatctgttc agcctcatgc aggtaggtat atacattact gtcgatccct
      181 accaggctaa gccggacgac atcatatctt tttacctcgc cggtatcggc gtcgatcctg
      241 tcatggatat accggccatc ggatagcctg tcctcgaagg tcagaaaacg tttgtccact
      301 gtcccgttga tcgtcatggt atacttataa aagttgatga tatgcaccgg atcctggtaa
      361 ttggcgacgg cctcgatcct ggtggtgtcg ctgaaatcaa agctaaccga gtccaacggc
      421 accggctgcg gcatggtgga agacgccgta taggtggtcc cgttgagccg cacattcaac
      481 agataggtat ggcccgggat gccttgcgtc agcgtatgtg ttacatagag acccgtgctt
      541 gtctccgtga gtgcatcctg ctgcccggtg gtggaatcgg tgatggtgac cgccgctccc
      601 gcgatgcccg ggtagatgct gctgtcatag aaaccgacag atctgacgat cgatacgaaa
      661 taaggccccg cggtatcgct caccgcaccc tgtatataaa cctgtgagcc ggcattattc
      721 agagcggggg tgatcgtctt ggtgcaggcg gcaaggatgg ctgctgccgt gaggatgaaa
      781 aatacgcttt tcatgatact taaaatttga aattataggt aatggaagga acgaaggtga
      841 acagggtcgt ctgctgcacc tgggtcagca gcttattggt tgggtcttgc tggaaggtga
      901 tcgtatatgg gttacgatag ccgtaagcgt tgtagatgga gaaattgaga tcgctttcaa
      961 actttggtgt cttcttcaac tggagggtcg ccccaagatc gaggcgctga taggcaggaa
     1021 gccggtaatc attgcgggcg gtgtaataat acaccggtgc gccgtccacg gggtatttgc
     1081 ccgaaggcca ggtggtgggg ttgccggtgt tatagacgaa gtccgcggaa aacgtccatt
     1141 tccggttgta tttatagatg cccaccaccg acagactatg ggtctggtcc tggactgccg
     1201 ggtaccagct gccattgttg accccggcga tctgtctttc ggtcctggat aacgtatagc
     1261 tgacccaccc ggtgagcctg ccggaggtct tgtggatata gttctcccag ccgtatcccc
     1321 ggccttttcc aaagagaagc tcggcctcta tattgttgtt gccgacgagg ttgacgccgt
     1381 tggcatagtc gatctggttt tgcatctcct tataatagac ctcggtggag aactcgtaca
     1441 tgcgatggtg gaaatagctg tagtagcccg ccgacacctg gtcggagatc tccggcttca
     1501 cgatattgct ggtcgggagg tagacattgg tcgggcttgc cgtcgtggca ttgttgagca
     1561 gatggatgtt ctgcaccgtc cggtcataag ccagcttgac tgagctggtg ttgttcaatt
     1621 ggtacgttgc cgctatccgg ggttcggggt tccaataggt gatgatctcc ttccctccgc
     1681 tgtaatactt ctttgtcagt atattaccct gctggtcata cgtataggca ttgcccggac
     1741 ccagtacggc cagttgcgtg agccggacgc catacacgag tttgaacctg gtgttgggag
     1801 accattcatt ggaagcgtaa atggcgttct ccatggcgta tttcttttcc agcgtgatat
     1861 cgttgaactt cgaattgcca ctggtagtgc tctgacccgg ttgtgtaatg tgataggtca
     1921 tgtccagtcc aatgtccagc ttgctatgag ggctgacata gtaattcagg tcctccttga
     1981 aatgataatc cgtgataccc gacctgacat aggtgttgga ccccaggctc gcgaattgcg
     2041 cgttatagtt gtaattgctg aagatgagcg aggcgttgga aaaaagccgg gtagagaaga
     2101 catggttcca gcgaagggtg gccgtttggt taccccagtc cacaccgttc ttgtatcgct
     2161 ggctcaacac atccttgccg aaataggcgg acaggaagat gcggtccttg ctgtcgaagc
     2221 tatagttggc cttcaggttg acatcgtaga aatagagctt gctgtgcttg acattggtat
     2281 cggaggccag tggggtgaag agatcgacat aactgcgccg ggcgcttacg atgaaggagc
     2341 tcttgtcctt tacgattggc ccctccaggt tgaggcggga tgcgatgagc ccgatgccgc
     2401 cgctgccgct cagccgctgg tcattcccgt ccttcatccg gatgtcttcc acggaggcca
     2461 gtcgcccgcc atagctggca ggcatgccgc ctttatacaa attcacatc
//
LOCUS       DATGFG010000074         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1124935, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000074 DATGFG010000000
VERSION     DATGFG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124935"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VKQ52_01400"
     CDS             <1..983
                     /locus_tag="VKQ52_01400"
                     /inference="COORDINATES: protein motif:HMM:NF045341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85857.1"
                     /translation="PRNHWMMINPGTSPENAPKAHGGSSLDAGTTMDNQMVFDIFSTV
                     IRAAEILQRHDAFIDTLRMLRSKLPVMHIGKYGQLQEWLEDVDDPNDHHRHISHLYGL
                     FPSNQISPYRTPELYAAARNTLVQRGDVSTGWSMGWKVNWWARMLDGNHAYKLIQTQL
                     SPLGVNPEGGGTYTNLFDAHPPFQIDGNFGCTSGITEMLMQSADGALFLLPALPDAWP
                     TGSIRGLRARGGFEIENIEWKDGRLTKVVVRSRLGGNLRLRAPNELHSSGGVRLRSAS
                     GINKNPFYQIEETPAPVISADAKLTVMKPKPTLLYDIATQAGTTYTFLSN"
     gene            999..>2921
                     /locus_tag="VKQ52_01405"
     CDS             999..>2921
                     /locus_tag="VKQ52_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HLZ85858.1"
                     /translation="MKLNCLVYGLFAGALAVMSAPGFSQTLPYQDPSLSTQARAKDLV
                     GRLTLEEKASLMLDESAAVPHLGIRKFNWWSEALHGLANNGNVTVFPEPIAMAASFDD
                     SLLFHVFDAVSDEVRARYNDARRQGLENKRFLSLSVWTPNINIFRDPRWGRGQETYGE
                     DPYLTSRMGMSVVRGLQGPQDTKYRKLYACAKHYAVHSGPEPERHVMNLYQVDPRDLW
                     ETYLPAFKALVTKADVREVMCAYQRLDDEPCCGNNRLLQDILRTDWGFKYMVVSDCGA
                     ISDFWQSHKVSSDAVHAAAKGTLAGTDVECGYNYAFKSLPAAVRKGLISEKEIDLHLM
                     RVMAGRFELGEMDPDSLVSWSKIPMSVVNSPEHQRLALDMARESMTLLQNKGSILPLA
                     NNIRKIAVIGPNADEAPVLWGNYNGTPVQTVTILQGIKAKLPRASIIYDRACDLTDQV
                     FIESVIAQTSIDGKPGIKATYWNNRTREGQPIATVEITSPLALTTAGQHPFATGVPLE
                     GFSAKYETVFKATKSGQLVFRVEGTGGNELKVNGVSLKRFGSWRANPNRVTLAVEEGK
                     EYRIEVDYTQREHWGANLKLDFGWEVPVSFDALINKLKDVDLVIFAGGISSRLEGEEM
                     PVDLPGFKGGDRTDIEL"
BASE COUNT          642 a          756 c          915 g          608 t
ORIGIN      
        1 accccaggaa ccattggatg atgatcaatc ccggcacttc cccggagaat gcgccgaagg
       61 cgcatggcgg ctcttcgttg gatgcgggga cgacaatgga taaccagatg gtcttcgata
      121 tcttcagcac cgtgatccgg gccgcggaga tcctgcaacg gcacgacgct tttatcgata
      181 cgctacgaat gctgcgcagc aagctaccgg tcatgcatat tggaaaatac ggccagctcc
      241 aggagtggct ggaggatgtg gatgatccca atgatcatca ccggcatatt tcgcacctgt
      301 acggtctttt cccttccaac cagatctcgc cttaccgtac gcctgaactg tacgccgcgg
      361 ccaggaatac cttggtccag cggggcgacg tctctacggg ttggagcatg ggctggaagg
      421 tgaactggtg ggcgcggatg ctggatggca atcatgctta taagctgatc cagacgcagc
      481 tttcgccatt gggggtgaac cctgagggcg ggggaaccta taccaacctg ttcgatgcgc
      541 atccgccatt ccagatcgac ggcaatttcg gctgcacctc ggggatcacg gagatgctca
      601 tgcaaagcgc ggatggcgcg ttgttcctgc tgcctgcgct gccggatgca tggccgacgg
      661 gcagcatccg cgggctgagg gcgagaggcg ggttcgagat cgagaatata gaatggaaag
      721 atggcaggct gacgaaggtg gtagtgcggt cccggctggg tggcaacctg cggttgcggg
      781 cgcctaacga gctgcattct tccggcgggg tccgtttgcg atccgcgtcc ggcatcaata
      841 agaacccttt ttatcaaata gaagaaacac ccgcgccggt tatttcggcc gatgcgaagc
      901 tgacggttat gaagccgaag ccgaccctgc tgtatgatat cgctacgcag gcgggtacta
      961 cctatacatt tttatcgaac taattattac agcttatcat gaaattgaat tgccttgttt
     1021 atggattgtt tgccggagcg ctggccgtaa tgagcgcgcc gggcttttct cagacattgc
     1081 cttaccagga tccgtcgctg agcacccagg cgcgggcaaa ggaccttgta ggccggctga
     1141 ccctggagga aaaggcgtcg ctgatgctgg atgaatcggc ggccgtgccc cacctgggga
     1201 tcaggaaatt taactggtgg agcgaagcgc tccatgggct ggccaataac gggaatgtga
     1261 ccgttttccc cgagccgatc gccatggcgg cctctttcga tgacagcctg ctgttccacg
     1321 ttttcgatgc cgtgtcggac gaggtgcggg cgcggtataa cgacgcccgc cggcagggat
     1381 tggagaataa gcgttttctc agtctttcgg tatggacgcc caatataaat attttccgtg
     1441 atccgcgctg gggccggggc caggagacct atggcgagga cccttatctc acttcccgta
     1501 tgggcatgtc cgtagtgcgc ggcctgcagg ggccgcagga caccaaatac aggaagctct
     1561 atgcgtgcgc caagcattat gccgtgcact ccggtccgga gccggagcgg catgtgatga
     1621 acctgtacca ggtggacccg cgcgatttgt gggagaccta cctgcccgcc ttcaaggcgt
     1681 tggtgacgaa agcggatgtc cgcgaggtca tgtgcgctta ccagcggctg gatgatgaac
     1741 cttgttgtgg aaataaccgc ttgttgcagg atatccttcg gaccgattgg ggatttaaat
     1801 acatggtcgt atcggactgc ggggccattt cggacttctg gcagagtcac aaagtatctt
     1861 cggacgccgt acacgcggcg gcgaagggca cactggccgg gacggatgtg gagtgcgggt
     1921 ataactatgc tttcaagagc ctgcccgcag ctgtgcgcaa ggggctgatc agtgaaaagg
     1981 agatcgacct ccacctgatg agggtgatgg cgggccgttt tgagttgggg gagatggacc
     2041 cggactcgct ggtatcctgg tcgaagattc cgatgtcggt ggtgaactct cccgagcacc
     2101 aacggctggc gctcgatatg gcgcgtgaat cgatgaccct gctgcaaaat aagggttcga
     2161 tcctgccact ggcaaataac attcgtaaga tcgcggtcat cggacccaat gcggatgagg
     2221 cgcccgtcct ctggggcaat tacaatggaa cgcccgttca aaccgtgaca atcctacaag
     2281 gcatcaaggc caaactccca cgggcaagta tcatttatga ccgtgcctgc gatctcacag
     2341 accaggtctt catcgaaagc gtgatcgcgc agacctcgat agatggcaaa ccgggaataa
     2401 aagctaccta ctggaacaac aggaccaggg aagggcagcc gatcgcgacg gtcgagatca
     2461 cttccccgtt ggctttgacg acggcgggac agcatccttt tgctacaggt gttcccctgg
     2521 aagggttttc cgcgaaatat gagacggtgt tcaaagcgac aaaaagcgga cagctggtat
     2581 tccgggtgga aggaacaggc ggtaatgagc tgaaagtgaa cggggtgtcg ctgaaaagat
     2641 tcggctcatg gcgagccaac cctaaccggg tgacgctggc ggtggaagag ggcaaagagt
     2701 accggatcga ggtcgactat acgcagcggg agcattgggg agctaacctg aaactggatt
     2761 tcggctggga ggtgcccgtg agcttcgatg cactgatcaa caaactgaaa gacgtagacc
     2821 tggtcatttt tgccgggggg atctcttccc ggctggaagg agaggaaatg ccggtcgacc
     2881 tgcccggttt caagggcggc gaccgtaccg atattgaatt g
//
LOCUS       DATGFG010000075         5013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_443649, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000075 DATGFG010000000
VERSION     DATGFG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443649"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VKQ52_01410"
     CDS             <1..256
                     /locus_tag="VKQ52_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HLZ85859.1"
                     /translation="GNGNSEKEQVWKMLQQILRIEGKPPAYDASDALAVAVCHFFQYR
                     KGEMLADAAKPAGKTTGAKGKKAGSWEAFIRQNPEKIRTR"
     gene            complement(267..656)
                     /locus_tag="VKQ52_01415"
     CDS             complement(267..656)
                     /locus_tag="VKQ52_01415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HLZ85860.1"
                     /translation="MTLFSRIIAGEIPSYKIAENDLFFAFLDIFPLREGHTLVVPKVE
                     VDKFFDMPDAYLTQLLVFARPIAQAIEKAFDCDRCGLSVVGLEVPHAHLHLVPIDTSD
                     DLNFTRHKLKLSPDQLKAAQEKILRHL"
     gene            complement(653..1126)
                     /gene="greA"
                     /locus_tag="VKQ52_01420"
     CDS             complement(653..1126)
                     /gene="greA"
                     /locus_tag="VKQ52_01420"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HLZ85861.1"
                     /translation="MSGFTYFTKDALDQLKAELQKLKTVDRQAASKAIAEAREKGDLR
                     ENAEYDAAKEAQGLLEAKIQKLEGDLANARVIDEGTIDTSKVSILTKVTVTNLTNKKQ
                     MTYHLVSEKEADLKVGKISVTSPIGKGLLGKHVGDVASVQVPAGNLQFKIENITV"
     gene            complement(1233..2156)
                     /locus_tag="VKQ52_01425"
     CDS             complement(1233..2156)
                     /locus_tag="VKQ52_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ85862.1"
                     /translation="MKKVILTAKVHDYLINTLTGKGYTVQYAPQITYEELKASLDGVE
                     GLIVTTRLKIDRAIIDGAPALRWIGRLGSGMELIDVEYAEARGILCVSSPEGNRNAVG
                     EQALGMLLGLMNRLFIAGAEVKEGKWIRDANRGTELSGKTVGIVGFGNAGGAFARVLA
                     GFDVTILAYDKYRFGFAEGNIREASLEQLARWADVISFHVPLTDETFHMAGDEFFNSL
                     EKKPWFINASRGKVHDTAALIRALANGKIAGAALDVLENEKLDTYTPEEREQLDWLLA
                     RPDVLITPHIAGYTHEAFLKMARVTLEKLGI"
     gene            complement(2173..2922)
                     /gene="rsmA"
                     /locus_tag="VKQ52_01430"
     CDS             complement(2173..2922)
                     /gene="rsmA"
                     /locus_tag="VKQ52_01430"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HLZ85863.1"
                     /translation="MYTLKKSLGQHFLRDENISRKIVAALREHPFTRLLEVGPGAGAL
                     TKYLLELEGIDLRAVELDVDKIQYLQATYPALQGKLVHASILDIEKPFAGKFTVIGNF
                     PYNISSQILFKMLDWKEDLETMTGMFQKEVAQRVAAKEGSKTYGVLSVLIQAFFEVQY
                     LFEVHEQCFNPPPKVKSAVIRLIPRPAPAFRDEKRFFLLVKTAFNQRRKTLRNAVRSL
                     FDEAALQDPFFDKRAEQLSVTDFARLTWKMR"
     gene            3027..4145
                     /gene="pdxA"
                     /locus_tag="VKQ52_01435"
     CDS             3027..4145
                     /gene="pdxA"
                     /locus_tag="VKQ52_01435"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLZ85864.1"
                     /translation="MSQPQQKPVIGISVGDVNGIGTEVIIKTFSDHRLLEMCTPLIFG
                     SNKLINFYKKSSPETSFNYQSTKDFSRVAPKQINIYTCWEEEIAIVPGQLTDVGGSYA
                     VKSLTAAVEALRDKKIQGLVTAPLHKKNTQSPSFDFTGHTPYLKKTFNVNDVLMLMVS
                     EGFRVGLVTEHVPVKDIAPLITREAILSKLTILRDSLVRDFGIDKPRIAVLGLNPHAG
                     DEGLVGREEEEIIKPAIKDAKHQMMVFGPYSADAFFARGYHNRFDAVLAMYHDQGLIP
                     FKSLAGSEGVNYTAGLPMVRTSPDHGTAFDIAGKNKADNTSFIAAIYACLDIIRNRQE
                     YDENRRNPLKKMGSVVLANAEDEKIDEAGRSTRRDLTF"
     gene            4441..>5013
                     /locus_tag="VKQ52_01440"
     CDS             4441..>5013
                     /locus_tag="VKQ52_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85865.1"
                     /translation="MKRNLLTGSLTVLSALLFQTLFAAPASTVDNFNSRPGVRLMEVK
                     GYLQGKCWFFSDFDINRSGWVPNIEGDGAMVSGTGASATERTGIYTPVLDLTAGTAVS
                     FSYSFNLPVKDRRWIRILAIDGNNNPVATLDSLELTGAESGKVYTYKHTISITGTYTL
                     CINYRGIGGSERIAIDELNIGAAPHYASTCM"
BASE COUNT         1161 a         1427 c         1307 g         1118 t
ORIGIN      
        1 cgggaacggt aattccgaga aagaacaggt ctggaagatg ctgcaacaga tcctgcgcat
       61 cgaggggaag ccgccggcct atgatgcctc cgatgccctg gccgtggcag tatgccactt
      121 ttttcaatac cgtaagggcg agatgctggc cgatgctgca aagccggccg gcaagaccac
      181 cggcgcaaag ggaaagaagg ccggcagctg ggaagcattt atccggcaga accccgaaaa
      241 gatccgcacc cgatgacata catcggctac aggtggcgga ggatcttctc ctgggcggct
      301 tttagctggt cgggactcag cttcaattta tgacgggtaa agttgaggtc atcgctggtg
      361 tcgatgggaa caagatgaag atgggcatgg ggtacttcca accctacaac ggagagcccg
      421 caacggtcgc agtcgaaggc tttctctatc gcctgcgcaa tgggccgggc aaataccagc
      481 agttgggtga gataagcatc cggcatatcg aagaatttat ccacttccac cttgggtacc
      541 accagggtat ggccttcccg gagaggaaag atatccagga aggcaaaaaa caggtcattc
      601 tcggcaatct tataggaagg gatttccccc gcgatgatcc tggagaagag tgtcacacgg
      661 ttatattttc aattttgaac tggaggttac cggcgggtac ctgcacactg gctacatcgc
      721 ccacatgctt gcccaggagt cccttaccga tgggggaggt gacggagatc ttacccacct
      781 tgaggtcggc ttctttctcc gatacaagat gataggtcat ctgttttttg ttggtgagat
      841 tggtgaccgt caccttggtg aggatggata ccttgctcgt gtcgatggtt ccttcatcga
      901 tgaccctcgc attggccagg tccccttcca gtttctgtat cttggcttcc agcaatccct
      961 gtgcttcctt ggcagcatca tattcggcat tttccctgag gtctcccttt tcgcgggctt
     1021 ccgcgatggc tttggatgcg gcctgacgat ccacggtctt caacttctgg agttcggcct
     1081 tcaactggtc caatgcgtcc ttagtgaaat aagtaaatcc actcataact cctcctttta
     1141 tttacaagag agaaaaaaaa tacaacgccc cggaaagatc caaggcattg cactagttcg
     1201 cttattatac ctgcaaaaat agggaaaaag gcctaaatcc cgagtttttc aagggtcacc
     1261 cgggccattt taagaaaagc ttcgtgggta tacccggcaa tatggggggt gatcaggacg
     1321 tccggccggg ccagcagcca gtccagttgt tcccgctcct ccggcgtata ggtatccagc
     1381 ttctcatttt ccagcacatc cagggccgcc ccggcgatct tgccgttcgc gagcgcccgt
     1441 atcaacgccg ccgtatcatg gaccttcccc cgggaggcat tgatgaacca gggcttcttt
     1501 tccagggagt taaaaaattc atccccggcc atatgaaagg tttcgtcggt gagcgggacg
     1561 tgaaagctga tgacatcggc ccacctggcc agttgctcca gggaggcttc ccggatattg
     1621 ccttcggcaa acccgaacct gtatttatcg taggccagga tcgtgacatc gaaaccggcc
     1681 aatacccggg caaaggcccc cccggcattg ccaaaaccga cgatccccac cgtcttgccg
     1741 cttagctccg ttccccggtt ggcatcacgg atccatttcc cctcttttac ctcggcgccg
     1801 gcgataaaaa gcctgttcat cagaccgagc aacatgccca gtgcctgctc tccaacggca
     1861 ttgcggttgc cttcgggact gctgacgcaa aggatccccc ttgcttccgc gtattcaacg
     1921 tcgatcagtt ccataccgct tcccaggcgg ccgatccacc ggagcgcggg cgcgccgtcg
     1981 atgatggccc ggtcgatctt caggcgggtc gtgacgatga gcccctcgac gccgtcgagc
     2041 gaggccttga gctcttcata ggtgatctgg ggcgcatact ggacggtata tcccttcccg
     2101 gtaagggtat tgataagata gtcgtgaacc tttgctgtaa gaatgacttt tttcattgta
     2161 tataaatatg gactaacgca ttttccaggt aagccgggca aagtccgtaa ccgataactg
     2221 ctcggcccgt ttgtcgaaga acggatcctg taaggcggct tcgtcaaaaa gggaccggac
     2281 ggcattgcgc agggtcttcc ggcgctggtt aaacgctgtt tttaccagca gaaagaaacg
     2341 cttctcatcc cggaacgccg gcgccggccg gggtatcagc cggataaccg cactctttac
     2401 ctttggaggc gggttgaaac attgttcgtg cacctcgaac aggtactgaa cctcaaaaaa
     2461 agcctggatc agtacgctga gcacgccata ggtcttgctg ccctctttcg ccgcgacccg
     2521 ttgggcaacc tccttctgga acataccggt catggtctcg aggtcctcct tccaatccag
     2581 cattttaaaa aggatctgcg aagagatatt gtaggggaaa ttgccgatca ccgtgaattt
     2641 gcccgcaaag ggcttttcga tatcgaggat actggcatgg accagtttcc cctgcagcgc
     2701 cggataggtg gcttgcaggt attggatctt atccacgtcc agctccaccg cccggagatc
     2761 gataccctcc agttcgagca ggtatttggt cagcgccccc gcgccaggtc cgacctccag
     2821 caaccgggtg aaggggtgct cgcgaagggc ggctacgatc ttccgggaaa tgttctcatc
     2881 ccggagaaag tgctggccaa gggatttttt aagggtgtac ataaagggct aaattacaat
     2941 tttcccggtt ggcggggcat cgccgccggc cggaggcatc cgactgcgca ataaatcgta
     3001 catttgaccc ctagaaaacc tagcgtatga gccaaccaca gcaaaaaccg gtgatcggga
     3061 tctccgtagg agacgtgaac gggataggaa cagaagtgat cattaagacc ttttccgacc
     3121 accggctgct ggagatgtgt accccgctga tcttcggctc gaacaaactc atcaatttct
     3181 acaagaaatc aagcccggag acgagtttca actaccagag cacaaaggat ttttcccgcg
     3241 tcgccccaaa acagatcaat atctacacct gctgggagga agagatcgcc atcgtgcccg
     3301 gccaattgac ggacgtgggt ggaagttatg ccgtcaaatc cctgacggcg gcggtggaag
     3361 ccctccggga caagaagatc cagggtcttg taacggcccc ccttcataag aagaatacac
     3421 aatcccccag cttcgatttt acgggtcata cgccctacct gaagaagacc ttcaatgtca
     3481 acgacgtgct catgttgatg gtatcggaag gcttccgggt cggcctggtc accgaacatg
     3541 ttcccgtaaa ggatatcgcc ccattgatca cccgcgaagc catcctctcg aagctgacga
     3601 tcctgcggga cagcctggta agggattttg gcatcgacaa accgcgcatc gccgtgctgg
     3661 ggctgaaccc gcatgcggga gacgaagggc tcgtcggcag ggaagaggaa gagatcatca
     3721 agcccgccat caaggatgcc aaacaccaga tgatggtgtt cgggccttat agcgccgatg
     3781 ccttctttgc ccgtggctac cacaaccgct tcgacgccgt gctggcgatg taccatgacc
     3841 aggggctgat ccctttcaaa tccctggccg gcagcgaagg ggtgaactat accgccgggc
     3901 tcccgatggt aaggacctcg ccggaccatg gcaccgcctt cgacatcgcg ggcaagaaca
     3961 aggcagataa cacctccttt atcgcggcta tctacgcctg tctcgatatc atccgcaacc
     4021 ggcaggagta tgacgagaac cgccgcaacc cgctcaagaa gatgggctcc gtcgttctgg
     4081 ccaacgccga agacgaaaag atcgacgaag cgggacgctc cacccggcgc gaccttactt
     4141 tttgaacatc ctctccatta ccggaggacc atcgataaac ccttgacagg caacaccttt
     4201 gtcagggatt gccttttttc ccggaggggg cttgcaaaaa cagaaactct gttctatctt
     4261 tgactcgtta cccactactg ggtataccac tccgaaatta caatctcccc gaaaaacaac
     4321 agtccatcat tcctccggtc gtcactgatc tcttaattta ttatagttcc ttcctttgaa
     4381 tagcccctgt gttaccaaac ggtctatcat ttaattaatc actctctgaa aattacaaag
     4441 atgaaaagga acttattgac aggctctctt actgtcctct ctgcacttct tttccaaaca
     4501 ctgtttgcag ctcccgcttc gactgtcgac aacttcaatt cccggcccgg ggtccggctg
     4561 atggaagtaa aagggtattt acagggtaaa tgctggttct tctccgattt tgatatcaac
     4621 cgcagcggct gggttcccaa tatcgaaggc gatggggcca tggtttccgg caccggcgcc
     4681 agcgctaccg aaagaacggg tatctatact cccgttctcg acctgacggc cggcacggcg
     4741 gtctcttttt cctatagctt caacctgccg gtaaaggatc gccgctggat caggatcctt
     4801 gccatcgacg gtaacaacaa ccccgtcgcc accctggaca gcctggaact gacgggcgcc
     4861 gaatccggca aggtctatac ctataaacat acgatcagta tcaccggaac gtatactctt
     4921 tgtatcaact accggggcat cggcggcagc gagcgtatcg ccatcgacga gctgaacatc
     4981 ggcgccgcac cgcactacgc gtccacctgc atg
//
LOCUS       DATGFG010000076         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1241040, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000076 DATGFG010000000
VERSION     DATGFG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241040"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1312)
                     /locus_tag="VKQ52_01445"
     CDS             complement(173..1312)
                     /locus_tag="VKQ52_01445"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HLZ85866.1"
                     /translation="MKLSTKLIHAGVEPDPSTGAIMTPIYQTSTYVQSAPGVHKGYEY
                     ARSQNPTRAALEAALAQIENGRHGLCFSSGVAASDAVLRLLVPGDEVIAANDMYGGTY
                     RLFTKIFEKTGIRFHFVNMQDANAIRPLINANTRLVWAETPTNPLMNVMDIAALAAIT
                     KEKGVLLCVDNTFASPYLQNPLDLGADIVLHSATKYLGGHSDVIHGCLVMNSDDLREK
                     LYFIQKSCGAVPGPQDCFLVLRGLKTLHVRMDRHCSNGEAIAHWLRSHPRVAQVYWCG
                     FEDHPGYAIAKKQMRGFGGMMSFTLKDDSLEAAKKVLSSTKLLALAESLGGVESLINH
                     PASMTHASIPREERIKNGLSDSLIRLSIGIEDVDDLKADLREAIG"
     gene            1594..>2016
                     /locus_tag="VKQ52_01450"
     CDS             1594..>2016
                     /locus_tag="VKQ52_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85867.1"
                     /translation="MTRRLLQKSLVIACCLFALVSGAFAATDLTISDKLLQAFHKTFP
                     DAQQVKWAETEDKYMVNFKQGDILTKVEYDKDGNFLNSLRYYTEKNLPVNLLCRLQKK
                     YADKKVFGVTELTTDASVEYYIKLEDAENWITVKSNVEI"
BASE COUNT          475 a          507 c          552 g          482 t
ORIGIN      
        1 aaaaaaaccg gcgatctcga tatcctgccg cctgttgtat tggtgcggga tgcttaccgg
       61 atcgttgagg ataaacgcgg ggcgattgta ttcggcgacc tttttgtcaa gaaaggcttg
      121 aagatcatcg gggcggactg agcccgcccg gggatcatcg gggcgggtgg atttaaccga
      181 tggcttcacg gaggtcggct tttagatcgt ccacgtcttc gatcccgatg ctcagccgga
      241 tgagcgagtc gctaaggccg ttctttattc tttcttcccg gggaatggag gcgtgggtca
      301 tgctggccgg gtggttgatc agcgactcta cgccgccaag gctctcggcg agggccagca
      361 gtttcgtgga ggacaacact ttctttgcgg cttcgaggct gtcatccttt aaggtaaagc
      421 tcatcatccc gccaaacccg cgcatctgtt ttttggcgat ggcataaccg ggatgatctt
      481 cgaagccgca ccagtagacc tgggcgaccc tgggatggct gcgaagccag tgagcgatgg
      541 cttcgccatt ggaacagtgg cgatccatcc ggacatggag ggtcttcagt ccgcgcagga
      601 cgagaaaaca atcctgggga ccgggaacgg cgccgcagct tttctggata aagtataatt
      661 tttcccgcag gtcatcgctg ttcatgacga ggcagccgtg gatcacgtcg ctatgtccgc
      721 ccaggtattt ggtcgcgcta tgcaggacga tgtcggcgcc caggtcaagc ggattctgca
      781 gataagggga ggcaaaagtg ttatcgacac agaggagaac ccctttttcc tttgttatgg
      841 cggccagcgc ggcgatatcc atgacattca tcagcgggtt ggtcggcgtc tccgcccaga
      901 cgagccgggt gttggcattg ataaggggac ggatggcatt cgcgtcctgc atattgacga
      961 aatggaaacg gataccggtc ttctcaaaaa ttttggtgaa cagccggtat gttccgccat
     1021 acatatcgtt tgcggcgatc acctcgtcgc cgggtaccag cagccggagc acggcatcgg
     1081 aagccgccac gccgctgctg aaacatagac cgtgccggcc attctctatc tgggcaagcg
     1141 cggcttccag cgcggcacgc gtggggttct gcgaacgggc gtattcatat cctttatgaa
     1201 cacccggtgc gctttgcaca taggtagagg tttggtaaat gggggtcatg atggcgccgg
     1261 tggatgggtc cggctcaaca cctgcatgta taagttttgt agagagtttc attcgataaa
     1321 gatacaaagt gaaaaccact cgtcccactt catccagttt aacaaaagct taacccccga
     1381 agtgcaacat gactcttacg gcttcgcgca tgttttcacc ggtttaaaaa gtggatttac
     1441 atcctttggt cgaaccccct ggcaggatgc tgtaaaccgc gccggacaag ggttgccgga
     1501 agtgtagaaa cgcttgactt cggtgttctt acactctagc tttacggtat caaacgaaac
     1561 ttgtttctca acatctttaa aaaaataaaa cgtatgacac gccgtctcct ccaaaaaagt
     1621 ttagtcatcg cttgctgcct tttcgctctt gtatcgggag cctttgcagc cacggatcta
     1681 accatcagcg ataaattatt acaggccttt cacaagacct tccccgatgc tcagcaggta
     1741 aaatgggcgg aaacggaaga caagtacatg gtcaatttca aacaagggga tatccttacc
     1801 aaggtggaat atgacaagga tggtaatttc ctcaactccc tgcgttatta cacggagaag
     1861 aatctccccg tcaacctcct ttgccgcctg caaaagaaat atgccgataa aaaagttttc
     1921 ggcgttaccg agctgacgac ggatgcatcc gttgaatatt atatcaaact ggaagatgcc
     1981 gagaattgga tcaccgttaa gtcaaatgtt gagatc
//
LOCUS       DATGFG010000077         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_559851, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000077 DATGFG010000000
VERSION     DATGFG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559851"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VKQ52_01455"
     CDS             <1..303
                     /locus_tag="VKQ52_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85868.1"
                     /translation="RLLSESYILWENSTYDRASPDPRFVDLVRTANSRVLTRMLYSPY
                     YYYAINAMEALTYMAATRQLTLAPALRDRIDRLRTPNLKTTDQQIIEKYRTALTAH"
     gene            complement(355..>2433)
                     /locus_tag="VKQ52_01460"
     CDS             complement(355..>2433)
                     /locus_tag="VKQ52_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ85869.1"
                     /translation="GFMADAGFFRLFTYHFIEGDARAALDQPSSMVVSEEIAHKLFGE
                     VPAVGKVIHVSSGINGDHDLTVRGVFRPMNVPSHIEANFFMSMRGGDFENVLRRQEND
                     FATNNMFVTYVKLRPGADPASLEGKFPAFTDKYAGKDLKSYGYTKRQFLVPVADLHLR
                     PDLNSSIAPTTSKTYLYILGSIAVFTLLIACINFMNLATARSAKRSSEVGVRKVLGAV
                     KRGLITQFLGESVLMSVIAFGLAWAFTVMLLPAFSMMTDRNLSFGLADHVFLLAGFLG
                     LAVFAGLLAGLYPAFYLSSFQPAKVLKGKFSNSLAAVSLRKGLVVFQFMISVTLIVST
                     VVINDQMRYLRDQDLGFNKSAQIVIPLRGAVAKQQDAALETEFRRNGQVMNVGGALYY
                     PGIINPSDNMMYREGEDPHLAKRVQLNFVDFDYARTLELATVAGRLFSREFTADTSGR
                     IILNESAAKNFGFATPQAAVGTRIKTDFQGQTTTFEVIGVVKDFHFEDLHVGVAPYAM
                     VYNRSRGSYLIVHTRSGDPGPVLSSLGAAWHKLDPNEPFEYSFLDEDFQKNYASEERL
                     SAMISYFTAMAILISCLGLFGLASFSAEQRIREIGIRKVLGASVAGIVLLLSKDFLKL
                     VAIAMLIASPVAWLIMHRWLQDFAYRVTIDWMVFLVTFTATLGITLVTIGFQAVRAGM
                     ANPVKSLKTE"
BASE COUNT          626 a          841 c          535 g          431 t
ORIGIN      
        1 cggctgctct ccgaatccta tattctctgg gaaaattcca cctatgaccg ggccagcccc
       61 gatccccgct ttgtggacct cgtccgcacg gccaatagcc gcgtactcac ccgaatgctc
      121 tattcccctt attattatta cgccatcaat gcgatggaag cgctcaccta tatggcagcc
      181 acccggcaat tgaccctggc ccccgccctc cgcgaccgga tcgaccggct gagaaccccc
      241 aacctcaaaa ccaccgacca acagatcatc gaaaaatacc gcaccgccct cacagcccac
      301 tagccgccag cgccatcacc ctcgcaaccc actaactacc gccggcccca atagctactc
      361 cgtcttcaaa ctcttgaccg ggttcgccat cccggccctc acggcctgga acccgatggt
      421 caccagcgtg atccccagcg tcgccgtgaa cgtcaccagg aaaaccatcc agtcgatcgt
      481 cacccggtaa gcaaaatcct gcaaccacct gtgcatgatc agccacgcca ccggcgaagc
      541 gatcagcatg gcgatagcca ccagcttcag gaaatccttc gacaacaaca gcacgatccc
      601 cgccacactc gcccccaata ccttgcggat accgatctcc cggatacgct gctccgcact
      661 gaaactggcc agcccaaaaa gcccaagaca ggaaatgagg atggccatcg ccgtaaaata
      721 gctgatcatc gccgaaagcc gttcttccga cgcataattc ttctgaaagt cttcgtcaag
      781 aaaagaatat tcaaaaggtt cattggggtc cagtttatgc caggccgcgc caagcgagga
      841 aagcaccggc cccggatcac cggaccgcgt atgcacgatc aggtaactgc cccgggaacg
      901 gttatatacc atcgcataag gcgcaacccc cacatgcagg tcctcgaaat gaaaatcctt
      961 caccaccccg atcacctcga aggtggttgt ctgcccctga aaatcggtct tgatccgcgt
     1021 acccaccgcc gcctgcggcg tggcaaaccc gaaattcttc gccgcactct cgttaaggat
     1081 gatccgcccg ctcgtatcgg ccgtaaactc ccgcgagaac aatcgccccg ccacagtggc
     1141 aagctccagc gtccgcgcat aatcaaagtc cacaaagttc agctgcaccc gcttggccag
     1201 gtgcggatcc tccccttccc ggtacatcat attgtcagaa ggattgatga tccccgggta
     1261 atacaaggcc ccacccacat tcatcacctg gccgttgcgc ctgaactccg tctccagcgc
     1321 cgcgtcctgc tgcttcgcca cggcgccccg caacgggatc acgatctgcg ccgacttatt
     1381 aaaaccaaga tcctggtccc gcaaataacg catctggtcg ttgatgacca ctgtcgaaac
     1441 gatcagcgtt accgagatca tgaactgaaa taccacaagc cccttccgca gcgacaccgc
     1501 cgccagcgaa ttggagaact tccccttcaa taccttcgcc ggctggaagg aagaaagata
     1561 aaaggccggg taaagccccg ccagcaaccc ggcaaaaact gccagcccaa gaaaacccgc
     1621 cagcaagaac acatgatccg cgagaccaaa cgaaagattc cggtccgtca tcatgctgaa
     1681 ggcgggcagc aacatcaccg taaaagccca ggccagtcca aaagcaataa cgctcatcag
     1741 gaccgactct cccaggaatt gcgtgatcag cccgcgcttc accgccccca acaccttgcg
     1801 gacacccacc tccgacgacc gctttgccga ccttgccgtc gccaggttca taaaattgat
     1861 acacgcgatc aataaggtaa ataccgctat cgaccccagt atatacaaat aggtcttgct
     1921 cgtagtgggc gcaatgctgc tgttcagatc cgggcgcaga tgaagatcgg ccactggcac
     1981 caggaactgc cgcttggtat aaccatacga cttcaggtcc ttacccgcat acttatcggt
     2041 aaatgccggg aacttgcctt ccagcgacgc cggatcggcg ccgggccgca gcttcacata
     2101 ggtcacaaac atattattcg tcgcaaaatc attctcctgg cgccggagaa cattctcaaa
     2161 atccccaccc cgcatactca taaaaaaatt ggcctcgata tgcgaaggca cgttcatcgg
     2221 ccggaagaca ccccgaacgg tgagatcatg gtctccattg atgccgctgc tcacatggat
     2281 caccttcccc accgccggaa cctcaccgaa cagcttatgc gcaatctcct ccgacaccac
     2341 catcgaagaa ggctggtcaa gggcagccct ggcatcaccc tcgataaaat gataggtaaa
     2401 aagccggaag aaaccagcgt ccgccataaa tcc
//
LOCUS       DATGFG010000078         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1586342, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000078 DATGFG010000000
VERSION     DATGFG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586342"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3865)
                     /locus_tag="VKQ52_01465"
     CDS             complement(<1..3865)
                     /locus_tag="VKQ52_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ85870.1"
                     /translation="MIKVLLSLRHRQLPPSLHFKELNDHIILEDSPFYVNDRLKEWVS
                     ENGQPRRAAVSSFGFSGTNAHVVIEEYANRSAGSAIDRSTNPANQANRPAGSAIVVLS
                     AKNAARLKEVMVNLLEHLSKYPGIDLHELAYTLQVGREAMAERWAAIVMDVNTLFLQL
                     TAFLEGQPGNFFAGNTRRDKTDPLTKEGARRPHNGQRDPDSVAGAWVKGEHVEWALLY
                     PGGKPGRISLPTYPFARERHWLPAADNKNNIPNKKEVKHVFVFTGQEYFLTDHLVLGE
                     KVLPGVAYIELVRQAASRSIPGITRIKEMNWLKPLLVKESPVTVYVSLLPLEEEYGYA
                     VYSVQREEEQIHCRGRLSRSSLAEPEKQTPAAIQSRLPDSMEGPACYDLFRQLGLQYG
                     KGFQGIKQLYYSGTEALSRISLPKRKVSFGASELESHATPSAGCILDPGILDSALQTV
                     AGLYLGKENISLALPFSAGEICIYKDLPETVWCYARISPAGSPGSKAMLCDIDLLNES
                     GEVLLRLTDFLALSPKRLARIKPGGSAEVQLYGSQWQESPPSMTGPVMGKDSSAPAMG
                     LDFLILLAGGVKDQADQLSGRLGRVVESIDGDTAAAYFTAVLAKVRQKVEQRSATQIL
                     VVCRQSDYLDYGFVSGLLKTANREQPAISGKVVIVEDAWMEDMGMLAGLLEGEMGRPE
                     EEVSYRQEKRWVKTWQAIPPARLSSGEGGCGIKEGGVYLITGGAGGLGLIFARHISQT
                     RDTRVILSGRGELSEERRVVVGAIRGAEYHRCDVSDRESVNNLITMIRSTAGRLDGVI
                     HSAGVIRDSFILKKTGAESESVLSAKISGTRHIDEATRSEGLDFMVLFSSMAAVTGNM
                     GQGDYASANAYMDNYAVWRNEQVSLGQRQGKTLSINWTLWEEGGMQMNAETARYLEQH
                     WGMQALPTAEGLCAFETLLRESLGQAMVCFGKERKMQGMQGGGGLSDSSAIETILIGL
                     CSELLKLNKADLDVEVNFTEYGVDSIMMMKMLNGMESRLAMTVDPSVIINYPTIRQLA
                     CYLSEEKAAVSARQHRQLRSRYEGPQAGAKEGSGKIAIIGMSCKLAGSDNCEEFWENL
                     RNGRDLIGETPVERWDPMRYYSGEGGLDKTYTTRGGFIRDAGLFDAKYFRISDRDAIS
                     MDPQQRIVLELTRELLSEAGYGREEISGSRTGVYIGAKDNNYVRNNYEFLPSGTHQHA
                     VVNNISNMIAARVSDFYNLKGSSEVIDTACSSSLVAIHRACEEIRSGRATMAIAGGIS
                     IMVDAFAHIGFSQAQVLSKDGRSYVF"
BASE COUNT          827 a         1293 c          798 g          960 t
ORIGIN      
        1 caaatacata gctcctccca tccttcgaca acacctgcgc ctggctgaac ccaatatggg
       61 caaacgcatc caccatgatc gatatccctc ccgctatcgc catcgtcgcc cggccgctcc
      121 ggatctcttc gcacgcccgg tggattgcca ccaacgaact cgaacaggcc gtatcgatca
      181 cttcactcga ccccttcaaa ttgtaaaaat cagatactcg tgccgcaatc atgttgctga
      241 tgttattcac taccgcatgc tgatgcgttc ctgacggtaa aaattcataa ttattccgta
      301 cataattatt gtccttcgct ccgatatata cgcccgtccg gcttccactg atctcctccc
      361 tgccgtatcc cgcttcactt aacaactccc gtgtcagctc caatacgatc cgctgctgag
      421 ggtccatcga aatagcatcc cggtcgctta tccggaaata cttcgcatcg aacaggcccg
      481 catccctgat aaaaccaccc cgcgtcgtat aggtcttatc caaaccccct tcccctgaat
      541 aatatctcat aggatcccat ctctccactg gcgtctcgcc aatcaggtcc cgcccattgc
      601 gcaggttctc ccaaaactcc tcacagttat cagatcctgc cagcttacac gacatcccga
      661 tgatcgctat cttcccgctc ccctctttag ctcctgcctg gggtccttca tagcggcttc
      721 ttagctgacg gtgctgcctg gctgataccg ccgccttctc ttctgacagg taacacgcca
      781 gctggcgtat cgtcggataa ttgatgatca ccgacgggtc caccgtcatt gctaatcggg
      841 actccattcc attcagcatc ttcatcatca tgatcgagtc cactccatat tccgtgaaat
      901 ttacctccac atccaggtcc gctttattca gcttcagcaa ctccgaacaa agcccgatca
      961 atatcgtttc gatcgccgaa ctatctgaca accctcctcc tccctgcatt ccctgcatct
     1021 tgcgctcctt tccgaaacat accatcgcct gacccaaaga ctccctcagc agggtctcaa
     1081 aagcacacag cccttcggct gtcggcagcg cctgcattcc ccagtgttgt tccaggtacc
     1141 gcgccgtttc cgcattcatc tgcatccccc cttcttccca taacgtccag ttgatgctca
     1201 gtgtcttgcc ctgtctttgc cccagcgaca cctgctcgtt ccgccaaacg gcatagttat
     1261 ccatatacgc attggctgat gcataatccc cctgccccat attccccgtt accgccgcca
     1321 tcgatgaaaa caataccatg aaatccagcc cttcggaccg cgtcgcctca tctatatgcc
     1381 gggtgcccga tatcttcgct gacagcacac tttctgattc cgctcccgtc tttttcaata
     1441 taaaactgtc ccggatcact cccgcactat ggatcactcc gtccagcctt cccgctgtgg
     1501 aacggatcat tgtaattaaa ttattcaccg actccctgtc gctcacatcg cagcgatggt
     1561 actccgctcc ccggatcgcc cctaccacaa cccgcctttc ctcactcagc tctccacggc
     1621 ccgacaggat cacacgcgta tccctggtct ggctgatatg acgcgcaaag atgagtccca
     1681 gcccgcctgc tcccccggta atcagataaa cccctccttc tttaatacca caaccccctt
     1741 cccctgaaga cagccgggcc ggggggatcg cctgccacgt cttaacccat cgcttctcct
     1801 gcctgtagct gacttcttct tccggccggc ccatttcccc ttccagtaat ccggccagca
     1861 tccccatatc ttccatccac gcatcttcca cgatcaccac cttgccgctg atcgcgggct
     1921 gctcccggtt tgccgtcttc agcaaccccg atacaaaacc atagtccagg taatccgatt
     1981 gccggcaaac caccagtatc tgcgtcgctg accgctgttc caccttctgt cttaccttgg
     2041 ccagcaccgc cgtaaaatag gccgctgcag tatccccatc gatcgattcc actacccgcc
     2101 ccaatcgccc gctcaactga tccgcctgat ccttcacgcc gccggccagc aggatcagaa
     2161 agtccaaccc cattgccgga gctgacgaat ctttccccat cacaggcccc gtcatggatg
     2221 gcggcgattc ctgccactga ctgccataca actgcacctc cgcgctccca cctggcttga
     2281 tccgcgcaag cctcttgggg gaaagcgcta ggaaatccgt cagccgcaat aacacttctc
     2341 ctgattcatt caacaaatcg atgtcgcaaa gcattgcctt gctgccaggg ctaccggcgg
     2401 gactgatacg ggcataacac cagaccgtct ccggcagatc cttgtaaatg caaatttcac
     2461 cggcactaaa ggggagtgca aggcttatat tttccttccc caggtacaga cctgcaacgg
     2521 tttgcagcgc gctgtcaagg atgcccggat ccagtatgca tccggcggaa ggagtcgcat
     2581 gactttccag ctcagaagcg ccgaaggaga cttttctttt cggcagggat atccgggaca
     2641 aggcttccgt tccgctgtaa tagagttgtt ttattccctg gaagcccttt ccatactgca
     2701 aacccaattg gcggaacaaa tcatagcatg ccggcccttc catggaatcc ggcaaacggc
     2761 tttggatagc cgccggggtc tgcttttccg gctccgccag cgaagagcgg ctcaacctgc
     2821 ccctgcaatg gatttgttct tcttctctct gaacactata aacggcataa ccatattctt
     2881 cttccaaagg cagcaggctg acatatactg tcaccggcga ttcctttacc agcagcggct
     2941 ttagccaatt catctctttt atccgggtga tgccgggtat gctcctgctc gctgcttgcc
     3001 gtaccagctc gatatatgca acgccgggca atactttttc acccaatacg agatgatccg
     3061 ttagaaaata ttcctgcccg gtgaatacaa agacatgctt cacctccttc ttattgggta
     3121 tattattttt attgtctgcc gcaggcagcc aatgcctttc ccgggcaaag ggataggttg
     3181 gcaaacttat cctgccaggt ttcccacccg gatacagcag ggcccattcg acatgctctc
     3241 cctttaccca tgcgcccgcc actgagtccg gatctctttg cccgttatgt ggtcgtctcg
     3301 caccctcttt cgtcaaggga tcggttttat ctctacgggt atttccagcg aaaaaattac
     3361 cgggctgccc ttcaaggaaa gcggttagct gcaggaagag tgtatttaca tccatgacga
     3421 tggccgccca tcgttcggcc attgcctccc gccctacctg taaggtatag gccagctcat
     3481 gcaggtcaat acccggatat ttcgaaaggt gttccagcaa attgaccatc acttccttca
     3541 accgggctgc attcttcgca gaaagtacga cgatcgcaga tccggccggc cggttcgctt
     3601 ggttcgccgg gttcgttgat cgatcaatgg ctgatccggc tgaccggttg gcatattcct
     3661 cgatgactac gtgtgcattc gtaccgctga acccaaagct gctcacggct gctcttctcg
     3721 gttggccatt ctccgaaacc cattccttca atcggtcatt tacataaaat gggctgtcct
     3781 ccaaaataat atgatcgttc aattccttga aatggagaga cgggggaagt tgccggtgcc
     3841 ggagggataa caatactttg atcattccgc atactcct
//
LOCUS       DATGFG010000079         5869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_101452, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000079 DATGFG010000000
VERSION     DATGFG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101452"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VKQ52_01470"
     CDS             complement(<1..429)
                     /locus_tag="VKQ52_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ85871.1"
                     /translation="MDSKEDKIKVMLFEDNSHLRESLTILIDSSDAFVCAAAFPDAGR
                     IISDITEYAPRLLLMDIEMPRISGIEASKLVKKTFPDLPILMLTAFDDDDRIFQSLCA
                     GGSGYLLKDSTPDQILDALMDTYQGASVLSPSVAKRVVRFF"
     gene            complement(451..2385)
                     /locus_tag="VKQ52_01475"
     CDS             complement(451..2385)
                     /locus_tag="VKQ52_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ85872.1"
                     /translation="MKYIFVLTGLFFTGFTGHGQSLSKQTVDSMANALRHSTSDSQKA
                     DLQAAISLQLAEAYPDSALRMARQGAAIALAAGAKTPYAKCLESVGWSYLRLEKNDSA
                     EYYMTQSISIFHSLHKDRDEAHVMVNLTTLYDYEENNEKLMEYLLKALALMEGLNDRL
                     GKAVAEQKIADCYMKQGLNEKAMPYLQEALQDFRELHQYAFLGAALNYQGQIYLAFKN
                     YDSAFISYREASQAYRQADFTAGEAAAAEEIANVFLEKSNRKLQHPYTDSALYYFQQS
                     YHIFSKAGRIVWQAYEQMYIGEVLILLKKYDEAARDLKEALQAFVTDKSPKFAYEATL
                     YLSKLYKETGDYKKAIDYLEQSGVYKDSMDATNRKETMANMFARYETDKKDKTIQLLN
                     AQKALADKELSRNKVIGRFSFSLVALIVALAVVLWKQVRIRHQLKEVQMRNQLAGDLH
                     DDIGSSLSSILLLSNMATRNQGGNDRKGALEKIGFTAREVMDKISDIVWTMNPQNDGG
                     TSIRERIEKLGLQVRDISSLQLTIAVSHRLEELKLPMDFRKNIFLICKEALHNIVKHA
                     EASCISLSIDLVHKDINLIIRDDGKGFDTTDHARGNGLGTMAQRAKACGGEFTIQSGR
                     GEGTVILVVLPVPHSRYIFS"
     gene            2543..3847
                     /locus_tag="VKQ52_01480"
     CDS             2543..3847
                     /locus_tag="VKQ52_01480"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLZ85873.1"
                     /translation="MKSIFNERFFLSITLLFLAGCKKSDPAAPSLAINGFSPSHGPEA
                     TTVVITGTGFSATPADDNVTLNNKTAMITSASSTQLVATVPRQAGTGKIVVTVHGKTL
                     EGGVFTYDYSDAQVTTLAGSGTIGTADGTGTAASFNSPGDLAIDANGNLYVADVGAST
                     IREVTPAGVVTTIAGGRIGFLDGASLNARFNGPNGLTIDAAGNLYVTDGSNNAIRKID
                     HAGNVTTIAGTGVQGNANGPAATAQFAYPDGLTMDGAGNIYVADHFSRIRKITTQGMV
                     STLTGMNTQGYLDGPLASAQFAVPRGMLFDSSGNLFVCDQANSLIRKITPAGNATTWA
                     GSTPGFADGSASAAQFNYPFAMATDSSGNLLVTDGQNNCIRMITPAGMVSTVAGNRTA
                     GYADGNKATTRFSGVTGIVVGKDGSIYVAEFLNHRIRKIVLE"
     gene            3871..5283
                     /locus_tag="VKQ52_01485"
     CDS             3871..5283
                     /locus_tag="VKQ52_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HLZ85874.1"
                     /translation="MHLQKLLKQHLLSACCLLLAGLCRGQGVGIGTTSPHASAQLDIT
                     STSKGVLLPRVSTAQRIAIANPAFGLLVFDTDKGSFYLFDGENWKAMIFGNDADIPPI
                     TRVPVNGSYPFETSSESVAISGNFAMGGNPFTRNDIINYGDGGQGEVYVFSKANNWTQ
                     TQLLQASDGASSTNNNFGFCIAMSGNLAVIGAPGTDIGANLRQGAAYVFSYTGGVWTQ
                     IQKLQAPGNTDGHFGFSVSTDGNTIAVGAPGNRAGGPGAGAVYTYIPNPLAGAGTPWI
                     LENTLTNAGGAAGDSLGTSVFLHNGWLVAGAPAANSGTGYACAYSQSGNNFVFAQVLL
                     SPSPSAHSRFGASVCVNGNPYIFIGSPGAGIAGGNNGAIDSYQLSGNWTNIIGPGPND
                     YPFYGGGSFIGLSADGGTMITSYYEASTNLRILEGLYGTTFFLLKVSEYNLANLYPLA
                     SDGYWIVYTTDGGFAFKNVQ"
     gene            5295..>5869
                     /locus_tag="VKQ52_01490"
     CDS             5295..>5869
                     /locus_tag="VKQ52_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85875.1"
                     /translation="MKLFYTLILSVLILPGVEAQNILFVPSGAALTTTGGVVITLQDM
                     DLQCDGTINQLPGNGTFVFNGTAGTGVSGVSRPLFDVLEIAKTGGAGISLNQSVNIGG
                     SVNFTSGLINLTGGNVYLQPAAFLKGESETSHITGTAGGYVEISGMLNAPTAVNPGNL
                     GAIITSTQNLGNTVIQRGHEPQNTGNGHSIAR"
BASE COUNT         1354 a         1616 c         1534 g         1365 t
ORIGIN      
        1 aaaaaatcga accactcttt ttgcaacaga cggggataat accgatgccc cctgataggt
       61 atccatcagc gcatcgagga tctgatcggg ggtggaatcc tttaacagat agccggaacc
      121 gccggcgcaa agcgactgaa agatccgatc gtcgtcgtca aaagccgtca gcatcaaaat
      181 cgggagatcg gggaaggtct tctttacgag tttgctggct tcgatgccgc tgatccgggg
      241 catttcaata tccatcaaca acagccgcgg agcatattcc gtaatgtccg aaatgatgcg
      301 gccggcatcc ggaaacgcag cggcgcagac aaaggcatcc gatgaatcga tcagtatcgt
      361 caacgattcg cgcagatggc tattatcctc gaataacatc actttgatct tgtcttcttt
      421 cgaatccatc ctccaaatta gtaattaaaa tcaggaaaat atataccgag aatgagggac
      481 cggcaagaca acaagaataa ccgtcccttc cccccgaccg gactgaatgg taaattcacc
      541 cccgcatgct tttgcccgct gcgccatcgt ccccagaccg tttccccggg cgtgatccgt
      601 cgtatcaaat cccttgccgt catccctgat gatcaggtta atatctttat gaaccaggtc
      661 gatggaaagg gatatacagg aggcctcggc atgtttgacg atgttgtgca gcgcttcctt
      721 gcagatgaga aagatatttt tcctaaagtc catgggcagc ttcagctctt ccagccggtg
      781 ggaaacagcg atggtcaatt gcagggaaga gatgtccctt acctgcagcc ccagtttttc
      841 tatccgctcc ctgatgctgg tgccaccatc attttgagga ttcatcgtcc agacgatgtc
      901 gctgatctta tccatgactt ccctggccgt aaaccctatt ttctcaaggg cgcctttccg
      961 gtcattccct ccctggttcc tggtagccat attgctcagc agcaggatgc tgctcaagga
     1021 gctgccgata tcatcatgca gatcgccagc cagctggttg cgcatttgca cttcttttaa
     1081 ttgatgccgg atacgaacct gcttccacag gacgaccgca agggcaacga taagcgcaac
     1141 gagggaaaaa gagaaacgcc cgatcacctt gttcctggac aattccttgt cggcaagcgc
     1201 cttctgcgcg ttgagcaatt gaatggtctt gtcttttttg tcggtttcat accgggcgaa
     1261 catattggcc atcgtttcct tccggttggt agcatccatc gaatccttat aaacgcctga
     1321 ctgttccaag taatcgatgg cttttttgta atcgcctgtc tctttataca acttgcttag
     1381 atagagggtc gcttcatagg caaactttgg cgatttgtca gttacaaaag cctgcaaggc
     1441 ctcctttagg tcccgggctg cctcgtcata cttcttcagc aaaataagga cctccccgat
     1501 atacatttgt tcgtaagcct gccatactat ccggcccgcc ttcgaaaaaa tatggtatga
     1561 ctgttgaaaa taatacaagg cagaatccgt gtaggggtgt tgtaatttcc tattgctttt
     1621 ttcaagaaat acgtttgcga tctcctcggc tgcggctgcc tcaccggctg taaaatccgc
     1681 ttgccggtaa gcctggctcg cctccctata gctgataaaa gcggaatcat aatttttgaa
     1741 tgccagatag atctggccct ggtagttaag cgctgcgccc agaaaggcat actggtgtaa
     1801 ttcccgaaaa tcctgcaaag cctcctgcag atagggcatt gctttttcat tcaagccctg
     1861 cttcatgtag cagtccgcaa tcttttgttc cgcaaccgct ttgcccaacc tgtcgtttaa
     1921 gccctccatc agcgccagcg ctttcagcaa atactccatt aatttttcat tattctcctc
     1981 gtaatcatac agggtggtga gatttaccat gacatgggct tcgtcgcgat ccttgtgaag
     2041 gctatgaaag atggagatgg actgggtcat gtaatattcc gcggagtcat ttttttccag
     2101 cctgagatag ctccacccga cggactccag gcatttggca tagggggttt ttgccccagc
     2161 tgcaagcgcg atggccgccc cttgtctggc catacgcagg gcgctgtccg gataggcttc
     2221 cgccagttgt agagaaatag cggcctgcaa atcggccttt tgggaatcgc tggtgctatg
     2281 ccgcagtgca ttagccatgg aatcgacggt ttgcttgctg aggctttggc catggccggt
     2341 gaaacccgtg aaaaatagtc ctgtgagtac aaaaatgtat ttcattaata aggtgtatag
     2401 agtcagggtg agcgaaatat agtaaaaaac cttcaactat ttctatccgg gtaccccctc
     2461 attctcggga ttgcctatcc gccggcaaat gttcactttt gtcggcagca atccaatatc
     2521 ggcaacctct aatccttatg caatgaaatc gatcttcaat gaacgcttct tcctgtcaat
     2581 aaccctgctt ttcctggctg gttgcaaaaa aagcgatccg gcggctccct ccctggccat
     2641 caacggcttc agcccctcgc acggccctga agccacgaca gtggtcatca ccggtaccgg
     2701 ctttagcgcc acgccggccg acgacaatgt taccctcaac aacaaaacag cgatgataac
     2761 cagcgccagc tccacgcagt tggtagcgac ggtgccccgg caggccggca cgggaaaaat
     2821 agtagtgacc gtccatggga aaacactgga gggaggtgtt ttcacctacg actattccga
     2881 tgcacaggtg acgaccctgg cgggttcagg cacgatcgga accgcagacg gcaccggtac
     2941 ggcagcgtcc ttcaacagcc ccggcgatct ggccatcgac gctaatggca acctgtatgt
     3001 agccgatgtg ggtgcgtcca cgatccggga agttacgcca gccggtgtag tcaccactat
     3061 tgccggaggg cgcatcgggt tcctggatgg cgccagcctg aatgcccggt tcaatggccc
     3121 gaacggattg accatcgatg ccgcgggtaa cctatacgtc accgacggaa gtaataatgc
     3181 catccggaag atcgatcacg ccggaaatgt taccaccatc gccggaacag gtgtgcaagg
     3241 taatgcgaac ggcccggcgg caacagctca atttgcctat cccgacggcc ttacgatgga
     3301 tggggccggg aacatctatg tggccgatca tttcagccgt atccggaaga taacgactca
     3361 ggggatggtt tccaccctga ccggcatgaa tacccaggga tatctcgacg gccctttggc
     3421 cagtgcgcag ttcgccgttc cccggggaat gctatttgat agcagcggca atctgtttgt
     3481 gtgtgaccag gcgaattcgc ttattcgtaa aattacaccg gccggaaatg ctaccacctg
     3541 ggcgggctcc actccgggat ttgcggacgg cagcgcttcg gctgcccaat tcaactatcc
     3601 ttttgccatg gcgacggact ccagcggcaa cctgctcgtc acggatgggc aaaataactg
     3661 tatccgcatg atcaccccgg ctggaatggt ttctaccgta gcgggtaatc ggacagccgg
     3721 gtatgccgac gggaataagg ccacgacccg gttctccgga gtgacgggca ttgtcgtagg
     3781 caaggacggg tccatttatg tagcggaatt tcttaaccat cggatccgga aaatagtgct
     3841 ggaataaaat tggtcaaatc aataactact atgcatcttc aaaagctact caaacaacac
     3901 ctcctttcag cctgctgtct tttgctggcc ggcctttgcc gggggcaggg tgtcggcatc
     3961 ggcacgacct cgccccatgc cagcgcccag ttggacatca ccagcaccag caaaggggtg
     4021 ctattgcccc gggtcagcac ggcccagcgc attgccattg ccaatcccgc gtttggtttg
     4081 ctggtctttg acacggacaa aggcagtttt tatcttttcg acggggagaa ctggaaggcg
     4141 atgatctttg ggaatgacgc cgatatcccg cccatcacac gcgtaccggt gaacggcagc
     4201 tatcctttcg aaacatcaag cgagagcgtg gcgatctccg gtaattttgc aatgggtggc
     4261 aatcccttca cccgtaatga tattatcaat tacggcgatg gcgggcaggg cgaggtgtat
     4321 gtcttcagca aggcaaataa ctggacccag acgcaattgc tacaggcttc ggatggagct
     4381 tccagcacga acaataattt cgggttttgc atcgcgatga gcggcaacct tgccgttatc
     4441 ggtgcgccgg gcaccgacat cggggccaat ctcaggcaag gcgccgccta tgtattcagc
     4501 tataccggcg gggtgtggac gcagatccag aaattgcagg ctccgggcaa taccgacggt
     4561 catttcgggt tcagtgtgag caccgatggt aataccatcg ccgtcggcgc accgggcaac
     4621 cgggccggcg ggccgggggc gggagccgtt tatacgtata ttcccaatcc cctggcgggc
     4681 gcgggcacac cctggatact ggaaaacacc ctcacgaatg caggcggtgc ggcgggcgac
     4741 agcctgggca cttctgtttt tctccacaac ggctggctgg tggcgggggc gcccgcagcc
     4801 aacagcggga caggttatgc ctgtgcatac agtcaaagcg gtaacaattt tgtctttgcc
     4861 caggtgctgc tgtctccatc gccttctgcc catagccgct tcggcgccag cgtttgcgtc
     4921 aatggtaacc cctatatctt tataggcagc ccgggagcag gcattgccgg tggaaacaat
     4981 ggcgccatag atagctatca gctttccggc aactggacaa acatcatcgg gcctggcccc
     5041 aacgattatc ccttttatgg cggcgggtcc tttatcgggc tcagcgccga tggcgggacg
     5101 atgatcacga gctactacga ggcgagtacg aacctccgca ttttggaagg gctctacggc
     5161 accacttttt tcctgctgaa agtaagcgaa tataatttgg ccaaccttta tccccttgct
     5221 tcggatggat actggattgt ctatactacc gatggggggt tcgcttttaa gaatgttcaa
     5281 taaacctttt gcggatgaaa ctattttata cgctgatctt atcggtgctt atcctgcccg
     5341 gcgtcgaggc acagaacatc ctgttcgtgc ccagcggagc ggcgctgacc acaacgggtg
     5401 gtgtggtcat taccttgcag gatatggacc tgcaatgcga cgggacgatc aaccaactgc
     5461 cggggaatgg aacattcgtt tttaacggaa ccgccggcac cggggtatcc ggcgtcagcc
     5521 ggcccctgtt cgatgtgttg gaaatagcca aaacgggtgg cgcaggcatc agcctgaacc
     5581 agtccgtcaa catcggaggg tctgtcaact tcacatcggg cctgatcaat ttaaccggag
     5641 gcaatgtata tctgcaaccc gccgctttcc tcaaagggga aagcgaaacc agccatatca
     5701 ccgggacggc aggtggttat gtcgagatca gcgggatgct caacgcaccg acggcagtca
     5761 atcccggcaa cctgggagcc atcatcacct ctacgcaaaa cctgggcaat acggtcatcc
     5821 agcgcggaca tgagccgcaa aacaccggca acggccatag catcgcccg
//
LOCUS       DATGFG010000080         5579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1469955, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000080 DATGFG010000000
VERSION     DATGFG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469955"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VKQ52_01495"
     CDS             complement(<1..400)
                     /locus_tag="VKQ52_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85876.1"
                     /translation="MNTDTTLDLNERHNLAIRIWHWTFFIVLTASLITVGLASTVFRT
                     RNNIALVQNQLRQKGVVVDQDQARAVAHEFNDKLWELHTWLGYILVFFLVARAIIEIA
                     GPGEERLRTRIKAALAFKPATVEQKGEQQHY"
     gene            complement(488..1378)
                     /locus_tag="VKQ52_01500"
     CDS             complement(488..1378)
                     /locus_tag="VKQ52_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ85877.1"
                     /translation="MKHYKTLHSLLVENGWPPPEHPLLAVVRCHKACTSGDREFTSDF
                     YMIGFKKLKSGVIRYGRTKYDHENGCMSFVRPRQVIEFIDLEFEEDAFLIFFHEDYLN
                     GHPLHSEIKKYGFFDYESSEALHLSPKEEKTVWELYFKIHAESTTNPDEYSRDIILTH
                     LDSILKYSQRFYKRQFINRTIISDHTVSKFNSILAGYFEQGQLETQGLPTVNYMASQL
                     NLSSRYLSDLLKEETGKTAIELIHIFLISEAKNLLKGPDHTVSETAYKLGFENLPYFS
                     RLFKKEVGLSPNQFRKQAMN"
     gene            complement(1454..2269)
                     /locus_tag="VKQ52_01505"
     CDS             complement(1454..2269)
                     /locus_tag="VKQ52_01505"
                     /inference="COORDINATES: protein motif:HMM:NF006114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ85878.1"
                     /translation="MQKVIFITGASRGFGKLWAEAFLKRGDKVAATVRNLDALADLKK
                     QYGNALLPIRLDVTDREASFAAIRQTKEHFGRIDVVINNAGYGLFGAIEETTEKEARD
                     QMEANFFGLLWVTQAALPIMRAQGSGHIIQLSSMLGVVTLPVLGIYNASKFAVEGLSE
                     TLATEVNGFGIHITLVEPNGFSTDWAGASAVQTHSIKEYDGVKAAFKEVASGDIWGKP
                     EATTGAILRLVDDPNPPLRLFLGKIALPLTKQVYTQKLATWEDWNEVATAAHG"
     gene            complement(2350..3258)
                     /locus_tag="VKQ52_01510"
     CDS             complement(2350..3258)
                     /locus_tag="VKQ52_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLZ85879.1"
                     /translation="MNVVSRFLLFLATVVLCHGITFSQSPDKKLEARLRPLIDTFHGV
                     AGVYVRNLRTGREVAINADTLFPTASIIKIPILVGIFDKIAQGAYTYEQPLVYRDSMA
                     RKGSGLMQFFKDSSITDLRTAISLMITNSDNTTAVWCERLAGGGVAINAWLEAHGFQN
                     TRLNNRTPGREANRQLYGWGQTTPREMARLVTMIRNGEVINPDVSQNMYRIMTHSFYD
                     EYALSAVPPFVQAASKQGMVDDSRSEAVLVNAPHGDYVFYIATKNNKDQRWVSDNEAW
                     RLARNVSALLWEYFEPRSPYKALPHL"
     gene            3437..4075
                     /locus_tag="VKQ52_01515"
     CDS             3437..4075
                     /locus_tag="VKQ52_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85880.1"
                     /translation="MICLKSLTTAGLCFLIAYRLPAQIGAQPGREVSDTLTKSRVSAL
                     LGSVSRKEFEDKAEKKTVQFTDYLKILCDKKASYEELDKAVEQAITLFVNEDAVVETS
                     SNNRNAVLRLRIRDYLKKVKQVQYDRIEVKWTHVQYVGDLKAGPDGNLYGTVSFEQEF
                     RGYRDGKLVYSDITVKHAIVVLKTFTKTVEGSSRRLWDVLLADIGVVATKSL"
     gene            4088..>5579
                     /locus_tag="VKQ52_01520"
     CDS             4088..>5579
                     /locus_tag="VKQ52_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ85881.1"
                     /translation="MENLRLKASGWGPFLSTGLLLSITLSGFSQTSIQDSISSRDASE
                     IRYKAEKLVRTELNELLNALSNSGYETQEVAESIHAAYSESRNRIFRDSLVTVEPDLN
                     PAVRSSAQSGDEPVGKYLKDIDILYRKSDLPTVQFSNIRCSPVKKNDNIYVKVYFQSH
                     FTGKSTVSDEDYAVMNRIAEISAGKEGNQWRLYIVRLAFFDPADTIGDVGNNMPIKYD
                     RLHVTGISGEAGEPAPGETGKGGEAKATPAQLAAALVDKGRMQENNRQYKEAIENYQG
                     ALRLDPGGNARLQPRIRELTNRYAMLSGLQAKYDAGYVKEALKDYTEAIRRSTGNSDY
                     YLGRGRCYEKLNEPKSLALAIKDYTQAYDLDHNNLAALRCRAELYARMGDNFKALTDY
                     IVYITVNRSDIAAYERKSEIHLLLKLNADAISDLDEALAVDPGAASVYYRKGLLLFDQ
                     NKEEKAWEDFDSARVHGLDSASRMAIEYNPLYKSALYKRGIARNGTG"
BASE COUNT         1270 a         1404 c         1635 g         1270 t
ORIGIN      
        1 ggtaatgctg ctgttccccc ttctgctcca ccgttgccgg cttaaaggcc agcgcagcct
       61 ttatcctagt ccgtagccgc tcttccccgg gaccggctat ttcaatgatg gcccgggcca
      121 ctaagaaaaa aacaagtata tacccgagcc aggtgtgcag ctcccatagt ttgtcgttga
      181 actcgtgggc gacggccctt gcctggtcct ggtcgaccac gacacctttt tgccggagtt
      241 ggttttgcac cagcgcgatg ttgttacgcg tacgaaatac ggtggaggcg aggccgacgg
      301 tgatgaggga tgccgtcaat acgataaaaa aggtccaatg ccagatacgg atggccaggt
      361 tatggcgctc gttcagatcc agggtagtgt cagtgttcaa gatgagccgt ttagggcgtc
      421 aagatagtaa attattcgcg gatgtcgcga gggtgcgggg cagccgggca agggggtcgg
      481 gccggcccta gttcatcgcc tgtttccgga actggttcgg gctgaggccg acctcctttt
      541 tgaacaggcg ggagaaatag ggcaggttct cgaatcccag tttgtaggcc gtctccgaaa
      601 ccgtgtggtc cggccccttc aggaggttct tcgcctccga gatgagaaag atatggatga
      661 gctcgatggc ggtcttcccc gtctcttcct ttagcagatc gctgagataa cgcgaggaga
      721 ggttcagctg cgaggccata tagttcaccg tgggcaggcc ctgggtctcg agctggccct
      781 gttcaaaata gccggcgagg atgctgttga atttggaaac ggtgtgatca gaaatgatcg
      841 tgcggttgat aaactgccgc ttgtagaacc gctgactgta tttcaaaata gagtcaaggt
      901 gcgtaagaat gatatcgcgg ctgtactcgt cggggttagt ggtggattcc gcgtggatct
      961 tgaaatataa ttcccagacg gtcttttctt ccttcggcga gagatgcagg gcttcactcg
     1021 attcatagtc gaaaaacccg tattttttga tctccgaatg aagaggatgg ccatttagat
     1081 aatcctcgtg aaagaagatc agaaaggcat cctcttcaaa ttcgagatct atgaattcga
     1141 tcacttgccg tggacggacg aaggacatgc atccattctc gtgatcatat ttcgtgcggc
     1201 catagcggat gacgccggat tttagtttct tgaacccgat catgtaaaaa tcactggtga
     1261 actcccgatc gcccgaggta caggctttat ggcagcggac cacggcgagc aggggatgct
     1321 cgggcggagg ccagccattt tcgaccaaca gcgaatggag agtcttatag tgtttcatgt
     1381 gcgttcaatt tacttattat tttccgtgtg cgggcgcggc aacctatgat ccacgtgcgg
     1441 ccgccgcaac ctattatccg tgtgcggccg tagcaacctc attccagtct tcccaggtgg
     1501 ccagtttctg ggtatagacc tgcttcgtaa gcggcagggc gatcttaccg aggaaaaggc
     1561 gaagcggcgg attaggatcg tccaccagcc ggaggatcgc acctgtggtc gcttcgggct
     1621 tgccccagat gtcgccggag gcaacctctt taaaagcggc ttttaccccg tcatattctt
     1681 ttatggaatg ggtttgaaca gcagaggccc cggcccagtc tgttgaaaaa ccattgggtt
     1741 ccaccagggt gatatggata ccaaaaccgt ttacctcggt ggcgagcgtc tcgctcagcc
     1801 cttcgactgc aaatttcgat gcattgtaaa ttcccagaac aggtagggtg actacaccca
     1861 acatgctgga caactggatg atgtgtccgc ttccctgtgc ccgcatgatc ggaagggccg
     1921 cctgggttac ccacaataag ccgaagaaat tggcctccat ctgatccctg gcctctttct
     1981 cggttgtctc ctcgatcgca ccgaacagac cataaccggc attattgata acaacatcta
     2041 tgcgaccgaa atgttctttt gtttgcctga tagcggcaaa gctggcttcc ctgtcggtga
     2101 cgtcgaggcg gatcggcagc agtgcattgc catattgctt tttgagatcg gcgagagcgt
     2161 cgaggttgcg gacagtagcg gctaccttat cgccgcgttt cagaaaggcc tcggcccata
     2221 atttcccgaa gccgcgggag gctccggtga tgaagatgac tttttgcatt ttgttctttt
     2281 ttatgggtca aaggtaccct cgcgccgggc catggtgttt gcacaaaagt cgggaaaact
     2341 gatacggatt tacaggtgcg gcagcgcctt gtaaggagaa cggggttcga agtattccca
     2401 cagcagcgcc gagacgttgc gggcgagccg ccaggcctca ttgtcggaaa cccagcgctg
     2461 atccttatta ttctttgtcg cgatatagaa aacatagtcg ccatgcggcg cattgacgag
     2521 tacggcctcg gaccgggagt cgtcgaccat cccctgcttc gaagccgcct gaacaaaggg
     2581 cggcacggca gacagcgcat attcatcata aaagctatgg gtcatgatcc tgtacatatt
     2641 ctggctgaca tcgggattga tgacctcccc gttgcggatc atcgtcacga gacgggccat
     2701 ctcgcggggg gtggtctgtc cccagccata gagttgccgg ttggcctcgc ggcccggcgt
     2761 ccggttgttc agccgcgtgt tttgaaagcc atgagcttcc agccaggcgt tgatggccac
     2821 accgccgccg gcgagccgtt cgcaccagac cgcagtggtg ttatcgctgt tggtgatcat
     2881 gagactgatg gccgtgcgca ggtcggtaat cgaactgtcc ttgaagaact gcatgagacc
     2941 cgatcccttt cttgccatgg agtcgcggta aacgaggggc tgctcatagg tgtacgcccc
     3001 ctgcgcgatc ttgtcgaaga tgccgacgag gatgggtatc ttaataatac tggctgtagg
     3061 aaagagggta tcggcattga tcgccacttc cctgcccgtc cggagattgc ggacatagac
     3121 gccggcgacg ccgtgaaagg tgtcgatcag cggccggaga cgggcctcca gttttttatc
     3181 cggagactgg gaaaacgtga tcccgtgaca aaggacaacc gttgccaaaa agagcaggaa
     3241 cctgcttact acattcatta acaagaaatt tgcttgtaat ttaggagata tttgccaaaa
     3301 tggcagatgg ggcccgcttt tgtggcccat aaattgattt tatacctcta ctacttccaa
     3361 tctgttaaag gtcagctccc gaccgtatcg taagagcttt acgccgtacc cgaagatccc
     3421 aatttcattt gtttctatga tctgtctgaa gtcattaacc acagcagggc tttgtttcct
     3481 catcgcctac cggcttcccg cccagattgg tgcacagccg gggagggagg tgagcgatac
     3541 cctcacaaag agccgggtca gcgccctgct ggggagcgtt tcccgcaagg agtttgagga
     3601 caaggcggag aagaagacgg tccagttcac ggattatctg aagatcctgt gtgataaaaa
     3661 ggcaagctat gaagaactgg acaaggcggt ggagcaggcg atcaccctgt tcgtgaacga
     3721 ggatgccgtg gtggagacct cgtcgaacaa ccggaatgcc gtcctccggc tccggatcag
     3781 ggattacctg aagaaggtga agcaggtgca gtatgaccgc atcgaagtaa agtggacgca
     3841 tgtacaatat gtgggtgatc tcaaggccgg tccggatgga aacttatatg gcactgtctc
     3901 gttcgagcag gagttcaggg gttaccggga cggtaaattg gtgtatagcg atatcaccgt
     3961 caagcatgct atcgtcgtac tcaaaacttt tacaaaaaca gtcgaggggt cgtcgcggag
     4021 gctttgggat gtgttgctgg ccgatatcgg cgtggtggcg accaaatcat tgtaattcgt
     4081 tgaaattatg gaaaatctcc ggttgaaggc aagtggttgg ggtccgttcc tgtcgacggg
     4141 actgttgctt tctatcaccc tatccgggtt ttcacaaacg tcgatacagg actctatttc
     4201 ctcccgggac gcctccgaga tccgctacaa ggcggaaaag ctggttcgaa cggagctcaa
     4261 cgaattattg aacgcgttgt ccaacagcgg ctacgagacg caggaggtag ccgaaagcat
     4321 ccatgccgcc tattccgaga gccgcaaccg catcttccgc gattcgctcg tgacggtgga
     4381 gcccgatctc aatcctgcgg tcaggagcag cgcccagtcc ggtgacgagc cggtggggaa
     4441 atacctgaag gatatcgata tcctttaccg gaagagtgat ctgccgaccg tccaattcag
     4501 caatatccgg tgcagtcccg tgaagaagaa tgacaatatc tacgtcaagg tgtatttcca
     4561 gtcgcatttt acgggcaaga gtacggtcag tgacgaggat tatgccgtca tgaaccggat
     4621 cgcggagata tccgccggga aggaggggaa tcagtggcgg ctgtatatcg tacggctcgc
     4681 ttttttcgat cccgcggata ccatcgggga cgtggggaat aatatgccga taaagtatga
     4741 tcgactgcat gtcaccggaa ttagcgggga ggcgggagaa cctgcgcccg gggaaaccgg
     4801 gaagggtggc gaggcaaagg caacaccggc ccagctggcg gcggcgctgg tcgataaggg
     4861 ccggatgcag gagaataacc ggcagtataa ggaagccatc gaaaattacc agggcgccct
     4921 ccggctcgat ccgggcggca atgcccgctt acagccccgg atcagggagc tgacgaatcg
     4981 gtatgccatg ctctcggggc tacaggcgaa atacgatgcg gggtatgtga aggaagcgtt
     5041 gaaggattat acggaggcga tcaggaggtc taccggcaat tccgattact atctcggacg
     5101 cggccgttgt tatgaaaaat tgaatgagcc caaatccctc gcgctggcga taaaggatta
     5161 cacccaggcc tatgatctcg accataataa cctggcggcc ctgcgttgcc gggcggagct
     5221 gtatgcccgt atgggagata atttcaaggc cctgacggat tatatcgttt acatcaccgt
     5281 caaccgaagc gatatcgccg cctatgaacg gaaatcggag atccatctcc tgctgaaatt
     5341 gaatgcggat gccatttccg acctggatga ggcgcttgcg gtcgaccccg gggccgcatc
     5401 ggtctattac cgaaaagggt tgctgttgtt cgatcagaac aaggaggaaa aggcctggga
     5461 ggatttcgac agcgcgcggg tgcatgggct ggacagcgcc agccgcatgg ctatcgagta
     5521 taatcctttg tacaagtcag cgctttacaa acggggtatc gcgcggaatg ggaccgggg
//
LOCUS       DATGFG010000081         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_101176, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000081 DATGFG010000000
VERSION     DATGFG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101176"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2845
                     /locus_tag="VKQ52_01525"
     CDS             <1..2845
                     /locus_tag="VKQ52_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ85882.1"
                     /translation="QRTEYRHRGPRSRPHRPRRSGVIGYGTRKKVNLTGAVSDITGAE
                     IAKSPVANISNALGGSMPGLIVNTRSGEPGADDAAFFIRGIGTLGSTAPLIVIDGIPD
                     RQGGFNRIDPQDIETFTVLKDATGAIYGARAANGVVLITTKRGISGKSQLSFNANASL
                     TQPTRVPKMLNSHDYAQSVNEYDALVGQQPTYTAAQLQKYQDGSDPLGYPNTNWWKTV
                     MRPWAFQTNDVMSLRGGSEKVRYYLSGQYLQQNSIYKGGSDFYTNKNVRANVDIQATP
                     TLRIGLDAHYRNEYRLTEGPQYGNAGDIFQELWSAYPYLVPRYPNGLVGVGIGGGPQN
                     SMVYVLNGDLGSSTYNYDFLQTKSSLSWDLDAITPGLRLDAYYAYDLFFYGYKGFNAQ
                     PPPAYSYNQATNSYIEVQSSIPPNLNDSTARTVDQLFNAKLGWQRKIGRSSLEAFVAY
                     EMSRETYTGLDAYKTGFLSNNVQELFAGSTIGATNNSGTIQTARQNYIGRLSYNYDDR
                     YLLDFNMREDGSPNFPKGKQYGFFPAVSGAWRISNESFFKSRVIDEFKVRGSWGQTGN
                     DAVSPFQYIQTYQLQAGQISQYLAAGYFYGANPSQVPGFVLGPTPNVNITWETATTTD
                     LGLDMRFLRNLTFDLDVFRSMRTHILVPPNEVVPLYTGISLPDENLGKVLSRGIEFQL
                     GWRKSPSKNFSYFINGNFTYAVNKVIYEAEPSTVPAYQRMTGHPVGSYLLYQAQGLYQ
                     DTATVNKTPHPLGSGPGDIRYADVNGDGKINGLDKVRTNLSSTPEIMYGFSFGGRYKW
                     FDATIFFQGQAAAQALLQPGGLNMAQQFYDGRWLHAGDNKYPRTFNGPTNATYGSNTY
                     QSTFWLLNDGFLRLKNVEIGYNFGSNGLFQRVKISSARIYISGNNLFSIDKWGPSFDP
                     ESPGTTSPGQVSGTNGRYYPQQRVLNLGLSVNF"
     gene            2868..>4231
                     /locus_tag="VKQ52_01530"
     CDS             2868..>4231
                     /locus_tag="VKQ52_01530"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ85883.1"
                     /translation="MNKLYMILGLAALTSTMSCHKVLDLQPTNSFSTNTVWTSPALVQ
                     VFVNEIYAESVLSFKDGGFAWGAQTDELFSNFNWCNENQYVQGQATPDNQKSSFPLNY
                     SSTINFWSTLYSTIQKTNVFFANIGQLDTLGNGAAIASMTAQVHFLRALCYFELLKRF
                     GGVPLITKVYTSTNNSYTESRASWDATANFILAEIAAALPGLQSSAPSGQEGVATTGA
                     ALALKSRLLLYSASPYFNTSNDMSRWQAAADAALAVINMSQYSLYGTSTTYGKIFTDF
                     FNPEVIFARVYGGVFEDRYNTVYRDLSPNGYNGYSAYNVLGQMVDAFQMKDGSAFSWS
                     NPTEAANPYQNRDPRLYADILTNGASFQGRPAQFWLGGLDSKLSALSPWNASKTGYTV
                     LKMVDSTYNFNVQPYSPAQWIVFRLSEIYLNYAEAEAQLGQTATALTYLNKIRERAGM
                     PDLTS"
BASE COUNT          984 a         1332 c         1082 g          833 t
ORIGIN      
        1 tcaacggaca gaataccgtc accgtggtcc tcgcagccgg ccgcaccgac ctcggcgaag
       61 tggtgtcatt ggttatggaa cacggaaaaa agtcaatctc accggtgccg taagcgatat
      121 caccggcgcc gagatcgcta aatcgccggt ggccaatatc tccaacgcac tgggcggctc
      181 catgccggga ctcatcgtca acacccgcag cggcgagccg ggcgcggatg acgcggcctt
      241 ctttatccgg ggcatcggca ccctgggcag cacggcgcca ctgatcgtga tcgacggcat
      301 ccccgaccgc cagggtggat tcaaccgcat cgatccgcag gacatcgaaa ccttcaccgt
      361 gttgaaagat gccaccggcg ccatctatgg agcacgggcg gccaacggcg tggtgctgat
      421 cacgacgaaa cgcggcatca gcggcaaatc gcagctgtcc ttcaacgcca acgcctcgct
      481 gacacaacct acccgggtgc ccaaaatgct caactcgcat gactacgccc aatccgtgaa
      541 cgaatatgac gcattggtcg gccagcaacc cacctataca gccgcgcaat tgcagaaata
      601 ccaggatggc tccgatcccc ttggctaccc gaataccaac tggtggaaga ccgtcatgcg
      661 accctgggcc ttccagacca atgatgtgat gtccctccgc ggcggcagcg aaaaggtgcg
      721 gtattatctc tccgggcaat acctgcaaca gaatagcata tacaaaggag gctctgattt
      781 ctataccaac aagaacgtcc gggccaatgt ggatatccag gccacgccca ccctgcgcat
      841 cggactggat gcccactacc ggaatgaata ccggctcacc gaagggccgc aatatgggaa
      901 tgcgggcgat atcttccagg aactctggag cgcctaccct tacctcgtgc cgcgctatcc
      961 caatggactc gtaggcgtcg gcatcggggg cggaccgcaa aacagtatgg tctatgtact
     1021 gaacggcgat ctcggcagtt ccacctataa ttacgatttc ctccagacaa aaagctccct
     1081 gtcctgggac ctcgatgcca tcacacccgg tctgcgcctg gatgcctatt atgcctatga
     1141 cctgttcttt tacggctata aggggttcaa tgcacagccc cctcccgcct atagctataa
     1201 tcaggcgacc aactcctaca tagaggtcca gtcctcgatc ccgcccaatc taaatgattc
     1261 cacagcgcgt accgtggacc agctctttaa tgcgaaactg ggctggcagc ggaagatcgg
     1321 ccgaagctcg ctggaagcat tcgtggccta tgagatgtcg cgcgagacat ataccggcct
     1381 cgatgcctac aagaccggct tcctcagcaa taacgttcag gaactgtttg ccggcagcac
     1441 catcggggcc acgaacaatt ccggaacaat acaaacagcc cgccagaatt acatcggccg
     1501 cctgtcatac aactatgacg accgctatct cctggacttc aacatgcgcg aggacggttc
     1561 gcccaacttc ccaaaaggga agcagtatgg cttcttcccc gcggtgtccg gcgcctggcg
     1621 tatctccaac gaatccttct tcaaatcccg ggtgatcgac gagttcaagg tacgcggcag
     1681 ctggggccag acggggaacg acgccgtgag cccgttccaa tacattcaaa cctatcagct
     1741 ccaggcggga cagatctccc aatacctggc agcgggttat ttttatggag ccaacccgtc
     1801 acaggtgccc ggcttcgtgc tggggccgac acccaacgtg aatatcacct gggaaacagc
     1861 gaccaccacc gacctcggac tggatatgcg tttcctccga aacctcacct tcgacctcga
     1921 tgtcttccgc agcatgcgga cccatatcct cgttccgccg aatgaagtgg tccccctcta
     1981 tactggcata agcctgccgg atgagaacct cggtaaggtg ctgagccggg gcatcgagtt
     2041 ccagctggga tggcgcaaaa gcccctcgaa aaacttctct tatttcataa acggtaactt
     2101 cacctacgcc gtcaacaagg tcatctatga agccgaaccc tctaccgtcc cggcctacca
     2161 gcggatgacg ggtcaccccg tgggctctta cctcctctac caggcgcagg gcctctacca
     2221 ggacacggca acggtgaaca aaacaccaca ccccctcggc tcgggccccg gcgatatccg
     2281 ctacgcggat gtaaatggag atggcaagat caacggcctg gataaggtgc ggaccaatct
     2341 ttcctccacg ccggagatca tgtacggatt cagcttcggc ggccggtaca aatggttcga
     2401 cgccaccatc ttcttccagg gacaggcagc cgcgcaggcc ctcctgcaac cgggcggtct
     2461 gaacatggcg caacagttct acgacggccg ctggctccat gccggcgaca acaaatatcc
     2521 ccgcactttt aacggaccga caaacgctac ctacggttcc aatacctacc agtcgacatt
     2581 ctggctcctg aacgacggct tcctgcggct gaagaacgtc gagattggct acaacttcgg
     2641 ttcgaacgga cttttccaac gggtaaaaat atcctcggcg cgtatctata tcagcggcaa
     2701 caatcttttc tcgatcgaca aatggggacc ctccttcgat cccgaatcgc cgggaacgac
     2761 ctctcccgga caggtctccg gaaccaacgg ccggtattac ccgcagcaac gggtgttgaa
     2821 cctgggcttg agcgtcaatt tttaagtact aacaaacaac gaccatcatg aacaagctat
     2881 atatgatcct tgggctggcg gcgctgacaa gcacaatgtc ctgccacaag gtattggatc
     2941 tccagccgac aaattccttt tccaccaaca cggtatggac cagccccgcc ctggtccagg
     3001 tgttcgtcaa cgagatctat gccgaatcgg tattgtcttt caaagacggc ggcttcgcct
     3061 ggggggcaca gaccgacgag ctcttcagca atttcaactg gtgcaacgag aaccagtacg
     3121 tccagggaca ggctactccc gacaatcaga aaagctcttt tccgctgaac tacagttcga
     3181 cgatcaattt ctggtccacc ctgtatagca ccatccagaa gaccaatgtg ttcttcgcaa
     3241 acatcgggca gctggatacc ctgggcaacg gtgcggccat cgcctcgatg acggcgcagg
     3301 tgcatttcct gcgggcactc tgttatttcg aactgttgaa gcgttttggc ggagtaccgc
     3361 tgatcaccaa agtctacacg tccaccaata acagctacac cgaaagccgg gcttcctggg
     3421 atgccacggc gaatttcatc ctggcggaaa tcgcggccgc cctgcccggt ctgcaatcat
     3481 ccgcgccttc gggtcaggaa ggggtggcca ccaccggcgc cgcgctggca ttgaagtcgc
     3541 gtctcctgct atactccgcc agcccctatt ttaatacgtc caacgacatg agccggtggc
     3601 aggccgccgc cgatgcggcc ctcgccgtca ttaacatgag tcaatattcg ctgtatggca
     3661 catccaccac ctacggaaag atctttacgg atttctttaa cccggaggtg atctttgcac
     3721 gcgtctacgg gggtgttttc gaggaccggt acaacaccgt ctatcgtgat ctcagtccca
     3781 acggctacaa cggatacagc gcgtacaatg tgctcgggca gatggtggac gccttccaga
     3841 tgaaagacgg ttcggccttc agctggagca atcccaccga agcggcgaat ccctatcaga
     3901 atcgtgatcc gcgcttgtac gccgacattc tcaccaacgg ggcctccttc cagggacggc
     3961 cggcacagtt ctggctgggt ggcctggatt ccaaactcag cgcactttca ccctggaacg
     4021 cctcgaaaac cggctacacc gtcctgaaga tggtggactc cacctataat ttcaacgtac
     4081 aaccctatag ccctgcccaa tggatcgtct tccggttatc ggagatctat ctcaactatg
     4141 cggaagcaga ggcccaactg ggacagacgg ctacggcgct gacgtatttg aacaagatca
     4201 gggaacgggc cgggatgccg gacctgacct c
//
LOCUS       DATGFG010000082         3972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_559143, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000082 DATGFG010000000
VERSION     DATGFG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559143"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VKQ52_01535"
     CDS             <1..246
                     /locus_tag="VKQ52_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85884.1"
                     /translation="AKPMDRRAVINGAVSGLIAIGGIIGIIFASEQWIPEIRALRDYT
                     LLVAMFVVMILLGIFISFISTHRSVVKYLKMKLDDLY"
     gene            377..685
                     /locus_tag="VKQ52_01540"
     CDS             377..685
                     /locus_tag="VKQ52_01540"
                     /inference="COORDINATES: protein motif:HMM:NF022741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HLZ85885.1"
                     /translation="MATTKKETSPAQKPAPKPAAAPAHRQQAPLFGKENYRWMLIGIV
                     LIALGLILMGGGKSKDPNVFNPKEIYSFTRITLAPILILGGFVVEIFAIFRKDKKESQ
                     "
     gene            692..1486
                     /locus_tag="VKQ52_01545"
     CDS             692..1486
                     /locus_tag="VKQ52_01545"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HLZ85886.1"
                     /translation="MTIIQAIILAVVEGLTEFLPISSTAHMKFTNPIIGVTSTPFSDM
                     FEIVIQLAAILAVVVVYYKKFFDFSRFNFYIKLIVAVIPAIIFGVLLKKHIDSALGNL
                     YFMAWVMVGGGVLFLFVDKWFTNPRIDDEQQVSNRNAFVIGCFQVLSILLPGFSRSAS
                     TIIGGMSQKLTRKAAAEFSFFLAVPTMLAASVKSFWDVHKEHPDVLSKENASLLGLSS
                     IVTFFVAIIVIRFFIGFLKKYGFKPWGYYRIIVGLVMLVLLWRGML"
     gene            1483..2952
                     /locus_tag="VKQ52_01550"
     CDS             1483..2952
                     /locus_tag="VKQ52_01550"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ85887.1"
                     /translation="MTQTASKRVINGWAMYDWANSVYNLVITTTFFPIYFTKATAHHF
                     RSDTIPFLGMRVNNSSLYSYALSAAYLVIALLYPVLTSIADTRGNKYRFMQFFCYMGA
                     LGCSALYLFDGSNVGLGVFCFILASMGYVGSLVFYNAYLPEIAAPEDQDRVSAKGFSY
                     GYIGSVLMQVLGFGLVMVMSDKPFLSLRLNFLLVGLWWAGFAQFTFAVLPRSNRQAAA
                     ATNVFKTGFAEVKKVYAEVKKLAVLKRYLRGFFFYSIGVQTVMTAATLFGSNLLKLDD
                     KKLIITIVLIQLVAIVGATWMSKLSARYGNIRVLMGVVVFWIMICISAYITAGQAENL
                     KPFHDKIAELQQQKEDLFAKKGQMTAEAFDRADADVEGELSRWQHDLEPHQEPIEYSF
                     YLLALGVGLVMGGIQSLSRSTYSKLMPPTKDTASYFSYYDLTEKISIVIGMFTFGFIN
                     QITGSMKNAIIFLIVFFIIGLAWLFSAQARQGRQPAPVI"
     gene            2964..3800
                     /locus_tag="VKQ52_01555"
     CDS             2964..3800
                     /locus_tag="VKQ52_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLZ85888.1"
                     /translation="MQLHTISTGFFKLDGGAMFGVVPKTLWNKVNPADENNLCTWAMR
                     CLLIEEGGKLILVDNGIGDKQDAKFFGHYHLHGDDTLDRSLAALGYHRSDITDVLLTH
                     LHFDHCGGSIVREGERLVPAFKNATYWSHARHWKWATAPNDREKASFLKENILPIEES
                     GQLRFVDEHKPVILPGLTVRMVNGHTDAMMLPQLQYKGKTLVFMADLLPSTGHFPIAW
                     VMAYDMFPLTSLQEKRSFLEEAAAGGYILFFEHDPRVECCIPEKTEKGIRPGAGFRLS
                     DL"
BASE COUNT          833 a         1140 c         1054 g          945 t
ORIGIN      
        1 gccaagccca tggaccggcg ggccgtgatc aacggagccg tcagcggact gatagcgatc
       61 ggcgggatca tcgggatcat ttttgcttcc gagcaatgga taccggagat ccgggccctg
      121 cgggactata ccctgctggt agccatgttc gtagtcatga tcctgctggg tatctttatc
      181 tcctttatca gcacccaccg cagcgtggtc aagtacctta aaatgaagtt ggacgacctg
      241 tactgagcgc tcgccgggtc accaccgaaa tttggcccgc tcgcgctcca ccgctaataa
      301 ttggccttcg gccggccgaa ccgtgttcgg ctttggaaga tttcctatat tgcagaccca
      361 aaatcatcct tttttcatgg caacgaccaa aaaagaaact tctcccgctc agaagcctgc
      421 ccccaagccc gcggcggctc ctgcccaccg ccagcaagcc cccttgttcg gcaaggagaa
      481 ctatcgctgg atgctcatcg gcatcgtcct tatcgccctg ggcctcatcc tgatgggcgg
      541 cgggaagagc aaggatccta atgtcttcaa tcccaaagag atatatagtt ttacccggat
      601 aacgctcgcc ccgatcctca tcctgggcgg ctttgtagtg gagatcttcg ccattttccg
      661 caaggacaag aaggaaagtc aataaggcat tatgactata atacaggcaa tcattctcgc
      721 ggtagtagaa ggactaacgg agtttttacc catatcctct acggctcaca tgaagtttac
      781 caacccgatc atcggggtta cctccactcc tttttcggac atgttcgaga tcgtgatcca
      841 gctggcggcg atactggccg tcgtggtagt ctactacaag aaattcttcg acttctcccg
      901 gttcaatttc tatataaaac tcattgtcgc cgttatcccg gcgatcatct tcggcgtcct
      961 gctgaaaaag catatcgact ccgcgctggg caacctgtat ttcatggcat gggtcatggt
     1021 cggcggcggt gttctcttcc tctttgtcga caaatggttt accaatcccc ggatcgatga
     1081 tgaacagcag gtatccaacc gtaatgcctt tgtcatcggc tgcttccagg tgctgtcgat
     1141 cctgctgccc gggtttagcc gcagcgcatc caccatcatc ggcgggatga gccagaagct
     1201 gacccgcaag gcggccgccg aattctcctt ctttctcgcc gtaccgacca tgctggccgc
     1261 ctcggtgaaa agcttctggg atgtccacaa agaacatccc gatgtcctga gcaaggagaa
     1321 tgcatcgctg ctcggcctga gcagcatcgt taccttcttt gtcgccatca tcgttatccg
     1381 gtttttcatc ggcttcctga aaaaatatgg tttcaaaccc tggggctact accggatcat
     1441 cgtcgggctc gtgatgctgg tcttactctg gaggggtatg ctatgaccca gacggcgtcc
     1501 aaacgggtta tcaacggttg ggccatgtat gactgggcca attcggtgta taatctcgtg
     1561 atcacgacca ccttcttccc gatctatttt acaaaagcga cagcccatca tttccgaagc
     1621 gacacgatcc ccttccttgg catgcgggtg aacaattcct ccctgtatag ctacgcgctg
     1681 tcagctgctt acctcgtgat cgccctgctc taccccgtcc ttacctcgat cgccgacaca
     1741 aggggaaaca agtaccggtt catgcagttc ttctgctata tgggcgcgct gggctgtagc
     1801 gccctctatc tttttgacgg cagcaatgtg gggcttggcg tcttttgctt tatcctggct
     1861 tcgatgggtt atgtcggcag ccttgtgttt tataacgcct atctgccgga gatcgccgcc
     1921 ccggaagacc aggacagggt cagcgccaaa ggcttctcct acggctatat cggcagcgtg
     1981 ctcatgcagg tgctgggctt tgggctggtc atggtgatgt cggataaacc ctttcttagc
     2041 ctccgcctca acttcctgct ggtgggtctg tggtgggcgg ggttcgccca attcaccttt
     2101 gctgttttac caaggtcgaa ccgccaggcc gccgccgcga ccaatgtctt taaaacgggg
     2161 ttcgcagaag tgaaaaaagt gtatgcggaa gtgaaaaagc tcgccgtgct caaacgctat
     2221 ctgcgcggtt tcttcttcta tagcatcggg gtacaaacgg tgatgacggc cgccaccctg
     2281 tttgggagta atctgttgaa gctcgatgac aaaaagctga tcatcacgat cgtcctgatc
     2341 cagctggttg ccatcgtcgg cgctacctgg atgagcaaac tctccgcccg ctacggtaat
     2401 atccgggtgc tgatgggcgt cgtcgtattc tggatcatga tctgtatctc ggcctatatc
     2461 accgccggcc aggccgagaa cctcaagccc ttccacgaca agatcgccga actgcaacag
     2521 cagaaggaag atctttttgc gaaaaaaggg cagatgacgg ccgaagcctt cgatcgggcc
     2581 gacgccgatg tcgaaggcga gctgtcgcgc tggcagcatg atctcgaacc ccaccaggag
     2641 ccgattgagt attcctttta cctgttggcg ctgggcgtgg gcctggtaat ggggggcatc
     2701 cagtccctta gccggagcac ctactccaag ctgatgccgc cgacaaagga tacggcctcc
     2761 tatttcagct attacgatct caccgagaag atctccatcg tgatcgggat gtttaccttt
     2821 gggttcatca accagatcac cggcagcatg aagaacgcca ttatcttcct gatcgtcttt
     2881 ttcatcatcg gtctggcctg gttattctcc gcgcaagccc ggcagggccg gcaaccggcg
     2941 ccagtcatct aagtttttgt cttatgcagc tgcacaccat ttctaccggc ttctttaaat
     3001 tggacggcgg ggccatgttt ggcgtcgtgc caaagaccct ctggaacaag gtcaaccccg
     3061 ccgacgagaa caatctttgc acctgggcca tgcgctgcct gctgatcgag gagggcggta
     3121 agctgatcct cgtggacaac ggcattggcg acaaacagga cgcaaaattc ttcggtcatt
     3181 atcacctgca tggcgacgac acgctcgacc ggtcgctggc ggcgctgggc tatcaccgaa
     3241 gcgatatcac cgatgtgctg ctcacccatc tgcatttcga ccattgcggc ggaagcattg
     3301 tccgggaagg cgaacggctg gtgccggcgt ttaaaaatgc cacgtattgg agccatgcgc
     3361 gacattggaa atgggcgaca gcacccaatg atcgtgagaa ggcttccttc ctgaaagaaa
     3421 atatcctgcc gatagaagaa agcggtcagc tgcggtttgt agatgagcac aagccggtca
     3481 tcctccccgg gcttacggtc cgcatggtga acgggcacac cgacgcgatg atgctgccgc
     3541 aactgcaata caagggtaag accctcgtgt tcatggccga tctcctgcct tccaccggtc
     3601 atttccctat cgcctgggta atggcctatg atatgttccc cctgaccagt ttacaggaaa
     3661 aacgcagctt tctcgaagag gcggcagcgg gtggctacat cctttttttc gagcacgatc
     3721 cccgggtgga atgctgtata ccggaaaaga cggagaaggg catccgcccc ggcgccggtt
     3781 tccggctgtc ggacctctag aacattcctg gctgctggcg ccggtttagg gcgttccctt
     3841 ccatttccat agcgtggcgg aagtctcccc atcgaggggt actgcctcat agtattgccg
     3901 gaggaaggcc tcccagtccg tatgcgcggt ctcgtcgagg aagaaccgcc gggcgttgga
     3961 gcaatcgatg at
//
LOCUS       DATGFG010000083         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_211858, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000083 DATGFG010000000
VERSION     DATGFG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211858"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VKQ52_01560"
     CDS             <1..379
                     /locus_tag="VKQ52_01560"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HLZ85889.1"
                     /translation="NGVWVCTFAEVKALAHVLRDGLIRIYTASKSQENKGDKMHLLYD
                     YLTSTEFSEQWKAIREGFLSMKQSIQKERDVMERLWKAREKQLEKVLLNAAHVRGSIE
                     GIAGKDAIDVQLLEDPDNDLLPE"
     gene            complement(404..1069)
                     /locus_tag="VKQ52_01565"
     CDS             complement(404..1069)
                     /locus_tag="VKQ52_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HLZ85890.1"
                     /translation="MALFYQHNINQETKLGIWYIEEPESFFLEKVPLKKDVSHPFKRL
                     QHLAGRYLLSYLFPEFPLEEIRIADTRQPFLASEKFHFSISHCGNYAAAIVSSNSRVG
                     VDIELISPRIERLAHKFLDEEEVHFFNEDYAHFLEQWGLRGRVFQEFLTLIWNAKEAI
                     FKWYGRGELDFRRHIKLEGSINLEGDWINLPFVFQKGKSIHLGVAGRIFEEQSLTLAW
                     LLT"
     gene            1164..1700
                     /gene="dcd"
                     /locus_tag="VKQ52_01570"
     CDS             1164..1700
                     /gene="dcd"
                     /locus_tag="VKQ52_01570"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HLZ85891.1"
                     /translation="MILADTRILEEMEKGTIKIEPYDRSCLGSNSYDVHLGKWLATYR
                     EHILDAKKHNEIEYFEIPEEGFVLYPHIFYLGVTQEYTETHAHVPFLEGKSSTGRLGI
                     DIHATAGKGDVGFCGNWTLEISVKQPVKVYKGMPVGQLIYFPVEGEIMEKYNQKKNAK
                     YNGQPNKPIESMMWKNSF"
     gene            complement(1697..>2254)
                     /locus_tag="VKQ52_01575"
     CDS             complement(1697..>2254)
                     /locus_tag="VKQ52_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HLZ85892.1"
                     /translation="EALMSIDPRFGYPSPAVKASFYSYLGDYLGFGGYYNPFTGEAQV
                     NTCQLPFTQPYTTCHEMGHQLGYARENEANFAGYLSARSSPDPAFRYSAYFDLWMYAT
                     GEMYSRDSVFVKDLVKQLHPAIRQDFRDLRAFSRKYANPLESRVWKLYGGYLRANRQP
                     KGMVTYTEVTAWLIAYAKNVGWDNL"
BASE COUNT          609 a          604 c          583 g          458 t
ORIGIN      
        1 caacggcgtc tgggtctgta cctttgccga ggtaaaagcc ctggcccatg tgttgcgcga
       61 cgggctgatc aggatctata cggcttccaa atcccaggag aacaagggcg acaaaatgca
      121 tctgctgtac gattacctca cgagcacgga attcagcgaa caatggaagg ccatccgcga
      181 agggttcctg tccatgaaac aatcgatcca aaaggagcgg gatgtgatgg agcgcctctg
      241 gaaggcccgg gagaagcagc tggaaaaagt actcctcaac gccgcccatg tccggggttc
      301 catcgaaggc atcgccggaa aggacgccat tgacgtgcaa ttgctggaag acccggataa
      361 cgatctgttg ccggaataac ccacccgaca taacacaccg gcatcaggtc agcagccagg
      421 ccagggtaag cgactgttct tcgaatatcc gccccgccac gcccaaatgg atgcttttcc
      481 ccttctgaaa gacgaacggc aggttgatcc agtcgccttc gaggttgatg ctgccttcca
      541 gctttatatg ccggcggaaa tccagctccc cccggccata ccacttgaag atggcctctt
      601 ttgcattcca gatcagcgta aggaattcct ggaatacgcg tccgcgcaac ccccattgtt
      661 cgaggaaatg cgcatagtcc tcgttgaaaa aatgcacctc ctcctcgtcg aggaatttgt
      721 gcgccagccg ctcgatccgg ggactgataa gctcgatatc cacccctacc ctgctgttgg
      781 agctgacgat cgccgcggca tagttcccgc aatgagaaat ggaaaaatga aatttctcgc
      841 tggccaggaa aggctgtctt gtatcggcga tcctgatctc ctccagtggg aactccggga
      901 aaagatagga caaaagatag cggccggcca gatgctgcaa tcgtttaaaa ggatgcgaaa
      961 cgtccttctt aaggggtacc ttctccagga aaaaggattc gggctcttcg atataccaaa
     1021 tccctaactt tgtctcctgg ttgatattat gttgataaaa cagggccatc tataaaatta
     1081 tattcacata ttcgcaatca agaaagtaaa caagaaagta aacaagaaag taaataagaa
     1141 agcaaactta accaaatttc aacatgatcc tagccgatac ccgtatattg gaagaaatgg
     1201 aaaaggggac gatcaagatc gagccttatg accggagctg cctgggaagc aattcctatg
     1261 atgtccacct gggaaaatgg ctggccacct accgggaaca tatcctggac gccaaaaagc
     1321 ataacgagat cgaatatttc gagatcccgg aggaagggtt cgtgttatat ccacacattt
     1381 tttacctggg agtcacccag gaatacaccg aaacccacgc ccatgtcccc ttcctcgaag
     1441 gcaagtcctc caccggccgg cttggcatcg atatccacgc cacggcgggc aagggcgacg
     1501 tcggtttctg cggcaactgg accctcgaga tctcggtcaa acaacccgta aaggtctata
     1561 agggcatgcc ggtagggcaa ttgatctatt tccccgtgga aggcgagatc atggaaaaat
     1621 ataatcagaa gaagaacgcc aaatacaacg gccagcccaa caagcccatc gagagcatga
     1681 tgtggaagaa cagcttttag agattgtccc agccgacatt tttggcatag gcgataagcc
     1741 aggccgtcac ctccgtatag gtaaccatcc cctttggctg acggttggcg cgcagatagc
     1801 cgccatacag cttccagacc cgggactcga ggggattggc atattttcgg gaaaaggccc
     1861 gcaggtcccg gaagtcctgc ctgatcgccg ggtgtaattg tttgacgaga tcctttacaa
     1921 aaacggaatc ccggctgtac atctcccccg tcgcatacat ccacagatcg aaataggctg
     1981 aataccggaa ggcagggtcc ggggatgagc gggccgacag gtagcccgcg aaattcgcct
     2041 cgttctcccg ggcataacca agttgatgcc ccatctcatg acaggtggtg tacggctggg
     2101 tgaacggcag ctgacaggtg ttcacctgcg cctcgccggt aaagggatta taataacccc
     2161 caaaccccag atagtctccc aggtagctat agaaggaagc tttaacagcc ggtgagggat
     2221 acccgaatcg tgggtcgatg gacataaggg cctc
//
LOCUS       DATGFG010000084         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_443868, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000084 DATGFG010000000
VERSION     DATGFG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443868"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VKQ52_01580"
     CDS             <1..234
                     /locus_tag="VKQ52_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85893.1"
                     /translation="GGTDPKFVGYSTPPNPNNRIIPASFDFHLQSGSPALGKGYTGTI
                     AGLPSGAPAPSNDIGAYVTDTSGGKGNRHAAGY"
     gene            333..1331
                     /locus_tag="VKQ52_01585"
     CDS             333..1331
                     /locus_tag="VKQ52_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HLZ85894.1"
                     /translation="MKKLIHLSLSLSLALVCRQASAQQYSFDKKISVPGDGGYDYMAI
                     DEANNHLFVSHGTSFNVIDLATEQPIATIDGLKGVHGIAIVNEVNKGFISDGKANAVV
                     VVDLTSFKIIKTISLTATDEDAIVYDPASKKVFVFNGDSKSTCVIDINTLELVKTIDL
                     GGGPEFAVSNGKGLLYNNIEDQNSLKVIDTKTLAVTTTWPLAPCGGPTGIAMDIPNQR
                     IFTGCRGNKGLSVLDAKTGTVITTLPIGAGVDAVVYDPSQKLIFASNGDATTTIIKQE
                     SADKYTVVQTLTTQPRAKTMAFDKKTKKIYLSAPQFEGNTRKIVPGTFAVYVYKPA"
     gene            1377..1841
                     /gene="msrB"
                     /locus_tag="VKQ52_01590"
     CDS             1377..1841
                     /gene="msrB"
                     /locus_tag="VKQ52_01590"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLZ85895.1"
                     /translation="MINWNDVLDYAGKGNPAPDRRVEKTDAEWKAILTPEQYRITRKK
                     GTEARFSGELCSRYEPGKYNCICCDTELFDSSIKFDSKTGWPSFTQPLKENVIKYIKD
                     TSFGMTRIEVECSVCDAHLGHVFPDGPPPSGLRYCINSESLKHVTAEAPADK"
     gene            1841..>2180
                     /locus_tag="VKQ52_01595"
     CDS             1841..>2180
                     /locus_tag="VKQ52_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ85896.1"
                     /translation="MDYKLTWRSLMKHKLFSFINIAGLSIGLSCCMLIVIYIYHELSY
                     DTNLPNVKRLYQVGGIFVTDGKEDRFPCSPAITAANLQQDFPEIEQTARMLTFSFFGE
                     YKTLLQYTRPD"
BASE COUNT          541 a          756 c          477 g          406 t
ORIGIN      
        1 ggtggcacag atcccaagtt cgtaggctat tcgactccgc ccaaccccaa caacaggatc
       61 atcccggctt ccttcgactt ccatctgcaa tccggctcgc ctgcccttgg caaaggctac
      121 accgggacca tcgccggcct gccttccggc gcgcctgccc ccagcaacga tatcggcgcc
      181 tatgtcaccg atacctccgg cggcaaaggc aatcgccacg ccgctggcta ctaaacgttc
      241 ttatctaccc gggcggcatc attaccgatg ccgccctttt tttcaccgca atcccgaatc
      301 cctacagttt ccccttttaa cattgttcca ttatgaaaaa gctcatccat ctctccctca
      361 gcctttccct ggccctcgtc tgccggcaag cgtccgcaca acaatacagc tttgacaaaa
      421 agatctccgt ccccggcgac ggcgggtatg actacatggc catcgacgag gcgaataacc
      481 acctcttcgt ctcccatgga acttctttca acgtcatcga cctggccacc gaacaaccca
      541 tcgccaccat cgacggtttg aaaggcgtac atggcatcgc catcgtaaac gaagtgaaca
      601 agggttttat cagcgacgga aaagccaatg ccgtcgtggt cgttgatctc actagcttca
      661 agatcatcaa gaccatctcc ctcaccgcaa ccgatgagga tgccatcgtc tacgaccccg
      721 cctccaaaaa ggtcttcgtc ttcaacggcg acagcaagag cacctgcgtc atcgatatca
      781 acaccctgga attggtaaag accatcgatc tcggcggcgg ccccgagttc gccgtatcca
      841 acgggaaagg acttctctat aacaatatcg aggaccagaa cagcctgaag gtcatcgaca
      901 caaaaaccct cgccgtaaca accacctggc cccttgcccc ctgcggcggc ccgacgggca
      961 tcgccatgga tatcccgaac cagcgcatct tcaccggctg tcgcggcaac aagggcctta
     1021 gcgtcctcga tgccaaaacg ggaacagtga tcaccaccct ccccatcggc gccggcgtcg
     1081 atgccgttgt atacgatccc tcccagaaac tgatcttcgc cagcaacggc gacgcaacga
     1141 cgaccatcat caaacaggag tccgccgaca aatacaccgt cgtccagacc ctgacgacgc
     1201 aaccccgcgc caaaaccatg gccttcgata aaaagacaaa aaagatctat ctgagcgcgc
     1261 cccaattcga agggaacacc cgcaagatcg tcccgggcac cttcgccgtc tatgtataca
     1321 aaccggcctg atgacctttt ttccgtaact tcaccatcgc cgcaaaacac gataccatga
     1381 tcaactggaa cgatgtcctc gactacgccg gcaaaggcaa cccggccccc gaccgccgcg
     1441 tcgaaaagac cgatgccgaa tggaaggcga tcctcacgcc cgaacaatat cgcatcaccc
     1501 gtaaaaaggg cacggaagcc cggttcagcg gtgaactctg cagccggtac gagccgggaa
     1561 agtacaactg catctgctgc gacaccgaac tcttcgattc ctccatcaaa ttcgattcga
     1621 aaacaggctg gcccagcttc acccaacccc ttaaggaaaa cgtcattaaa tacataaagg
     1681 acacctcctt cggcatgacc cgcatcgagg tggaatgcag cgtctgcgac gcccacctgg
     1741 gccatgtctt ccccgacggg ccgccgccaa gtggcctgcg ctactgcatc aattcggaat
     1801 ccctgaagca tgtcacggct gaagccccgg cagacaaata atggactaca aactcacctg
     1861 gcgcagcctg atgaagcaca aactgttctc cttcatcaac atcgccggcc tctccatcgg
     1921 cctgtcctgc tgcatgctga tcgtcatcta catttaccac gaactctcct atgacaccaa
     1981 cctcccgaac gtcaaacgcc tctaccaggt gggcggcatc ttcgtcaccg atggaaagga
     2041 agaccgcttc ccctgctccc cggccatcac cgccgccaac ctgcaacagg acttcccgga
     2101 gatcgaacag acggcaagga tgcttacctt tagtttcttc ggggaataca agactctcct
     2161 gcaatatacc cgccccgacg
//
LOCUS       DATGFG010000085         6788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1355130, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000085 DATGFG010000000
VERSION     DATGFG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355130"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VKQ52_01600"
     CDS             <1..655
                     /locus_tag="VKQ52_01600"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85897.1"
                     /translation="VKKQLKLIQLLDPPFDSKELNPGYIKGYLPGVRENGGQYTHAAV
                     WTLMAYAALGQREKLWPLLELIHPINHATDTAGVNTYKTEPYVMAADVYANESHKGMG
                     GWTWYTGSAGWMYQFVLESFAGLERRGPLLQLRPCFPLHWPSIYLAYRYGNSEYRITI
                     FQVSDGSASRWKMGDQQGDGNTIRLEDDGQSHSVEMYYAVLKNRTKSEFHNVKVIHP"
     gene            complement(669..1094)
                     /locus_tag="VKQ52_01605"
     CDS             complement(669..1094)
                     /locus_tag="VKQ52_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ85898.1"
                     /translation="MTEVSIVAGRENMDVAMVHRFLNEDSYWAKGISYELVDQSLANS
                     FCAGAFIDGRQVAFGRVITDYSTFGWFADFFVLPAHRGKGISKMLLSFIAEQPWYMRL
                     RRKMLNTRDAHGLYRQFQFQNLSSPANVLEIYNPTVHLQ"
     gene            1189..2604
                     /locus_tag="VKQ52_01610"
     CDS             1189..2604
                     /locus_tag="VKQ52_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLZ85899.1"
                     /translation="MKQEFLYIAISNNIARLIRTDALKAGDRLPSVRRLCREHGISMN
                     TAKRVYLELEGQSLIESKPQSGYFVTRTPQKKLPLPETSRPSHSSSNREPGELIRKVY
                     FTMGKKDFTHFSIGVPSDELLPLRGLNKEMMHATRQLQGGGTRYEEWQGNEKLRRIIA
                     ARSFSWGGSLHENDLVTTAGGMNALSFCMMALGKPGDTIAVESPVYPGILQLAVSLGL
                     KVLELPTHPNTGVEIEALKKALPRIDLCLLVTNFGTPLGSCMPDENKREVVALLAKQG
                     IPLIEDDLYGDLHFGDQRPKCCKAFDKQGTVLWCSSISKTLAPGYRVGWVAPGIYKEQ
                     IMKLKLIHAISSPTITQEVVAGFLKSGRYENHLRKMRRILQDNYHHYAGTIAGYFPEG
                     TKTSRPQGGLALWVELDKKIDTAELYDIAIKHKISIAPGRMFTLQDQFQNCMRLCIGL
                     PWSENLQHKLQLLGRLAKMIR"
     gene            2698..3135
                     /locus_tag="VKQ52_01615"
     CDS             2698..3135
                     /locus_tag="VKQ52_01615"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ85900.1"
                     /translation="MSKVSIYLNFEGNTEEAFNYYKSVFKTEFTAPLMRMKDVPPQPG
                     MPPLPEKEANAVMHVALPILGGTELQGTDMLESLGHKLKIGNNTTICLEPDSKEETDR
                     IYNALSAGGSDTMAPQDMFWGAYWGTCLDKFGIRWMFNFTYKK"
     gene            3606..5813
                     /gene="katG"
                     /locus_tag="VKQ52_01620"
     CDS             3606..5813
                     /gene="katG"
                     /locus_tag="VKQ52_01620"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HLZ85901.1"
                     /translation="METLTSTSKCPFHNGTLKPGAGGGTRNSDWWPNSLKLHILRQHA
                     SEFNPMGEKFDYAKEFRTLDLKGVKKDIFQLMTTSQDWWPADYGHYGPFFIRMAWHSA
                     GTYRIHDGRGGAGAGMQRFAPLNSWPDNANLDKARLLLWPVKKKYGRKISWADLMILA
                     GNCALESMGLKTFGFAGGRADVWEPQEDVYWGSETEWLGDKRYTGDRVLENPLGAVQM
                     GLIYVNPQGPNGKPDPLAAARDIRETFRRMAMNDYETVALIAGGHTFGKTHGAADADK
                     YVGREPAAATLEEQGLGWKNSFGKGNADDTITSGLEGAWTTTPTKWSNNFFENLFGFE
                     WELTKSPAGAHQWKPKAGAGANTVPDAHDPSKRHAPTMLTTDLSLRIDPAYEKVSRHF
                     YEHPDEFADAFARAWYKLTHRDMGPKARYLGPEVPAEELLWQDPVPAVTHPLIDAKDI
                     AALKDKVLSSGLSVSQLVSTAWASAATFRGSDKRGGANGARIRLAPQKYWEVNNPPQL
                     SKVLDTLSGIQQEFNKAQSGGKKVSLADLIVLAGCAGVEKAAKNAGLDVQAPFTPGRV
                     DASQEQTDVESFAVLEPAADGFRNYLGADAPASAEEMLIDKAQLLTLTAPEMTVLIGG
                     MRVLNTNFDQSAHGVLTNTPEALTNDFFLNLLDMGTTWKAASETMQVFEGRDRASGKI
                     KWTGTRVDLIFGSNSELRALAEVYGCEDSKEKFVQDFLAAWNKVMDLDRFDLV"
     gene            complement(5879..>6788)
                     /locus_tag="VKQ52_01625"
     CDS             complement(5879..>6788)
                     /locus_tag="VKQ52_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ85902.1"
                     /translation="YWNVYTGTPLTSPVNVRFFFNPADTLAARNAAKAFKTSSGAALM
                     SNLEWFKTVGNAFTPDSLTATPKANVKGATIMLAPVYGTKDGVNYAEFDGVTSFSGGT
                     GIYIVSNTMVVLPVGLASFTGRQVNTTVLLNWTTPPGAGNLRFEIGRSADGLDWETIG
                     QVSAAGNSANLASYSFTDMHPIGYPRDNYYRLALVDKDGRYSYSGVVLVRMSGDGAAV
                     PQLDRIVPNPFGSDMEIDCTLPAGGPVEVQLRDMAGVILMRREYTANKGENVFRLTGL
                     NGLAQGMYIVRVVQGGLATIGKTIKR"
BASE COUNT         1725 a         1907 c         1741 g         1415 t
ORIGIN      
        1 tgtcaaaaaa caactgaagc tgatccaact cctcgatccc cctttcgatt caaaagagct
       61 aaaccccggc tacataaagg gctatttgcc tggagtacgg gagaatggcg gccagtatac
      121 ccacgccgcg gtctggaccc tcatggccta tgccgcattg ggtcaacgcg agaaactatg
      181 gcccctgctg gaattgatcc atccgataaa ccatgccacc gacaccgccg gggtcaatac
      241 ctacaagacg gaaccgtatg taatggcagc cgatgtctat gcaaacgaat cgcataaggg
      301 catgggtggt tggacctggt acacggggtc ggcgggatgg atgtaccagt ttgttttgga
      361 atcctttgcc ggattggaaa ggcgaggtcc gcttttgcag ttgaggccct gcttcccgct
      421 tcactggcct tccatttacc tggcgtaccg ctatgggaat tccgaatacc gcatcaccat
      481 cttccaggtt tcggacggct ccgcctccag gtggaaaatg ggtgatcagc aaggtgacgg
      541 gaatacgata cgcctggaag acgacggcca aagccacagc gtcgaaatgt attatgcagt
      601 cctgaagaac aggacaaagt cagaatttca taatgtcaaa gtcattcatc cataaattcc
      661 ggaacaggtc attgtaaatg aacagtaggg ttatagatct ccaggacatt ggcagggctg
      721 gaaaggtttt gaaactgaaa ttgacgatat aacccatgag catctcttgt attcagcatc
      781 ttccttctca aacgcatgta ccagggctgt tctgctataa aggacaatag catcttggaa
      841 atacctttcc cacgatgcgc cggcaataca aagaaatcgg cgaaccaacc gaaggtagaa
      901 taatccgtga tcaccctccc gaatgcgacc tgccgcccgt cgataaaggc gcctgcacaa
      961 aaggaattgg ccagcgattg gtctaccagc tcataagaaa tgcctttggc ccagtaggag
     1021 tcttcattca gaaaacgatg taccatggca acatccatat tctccctgcc tgcaacaatg
     1081 cttacttctg tcatatgctt ttacgatttg tatacaaaaa taaacaaggg ggcgaaatga
     1141 gcacagatac agttttgtat ctttcaatag acacagttaa ctttacacat gaaacaggaa
     1201 tttctgtata tagccatctc gaacaacatc gccagactga tcagaactga cgcgctaaag
     1261 gccggcgacc gcctgccctc ggtcagaagg ctgtgccggg aacacggcat cagcatgaac
     1321 accgcaaaaa gagtatacct ggagctggaa ggccaatccc tgatcgagtc aaaaccccag
     1381 tccggatact ttgtaacccg gacaccccaa aagaagcttc ctcttccgga gaccagcagg
     1441 ccttcgcata gcagcagtaa tagggaaccc ggcgaattga tccggaaagt ctacttcacc
     1501 atgggcaaaa aagactttac tcatttttca atcggcgtgc cttcggatga gttactgccc
     1561 ctgcggggtt taaataagga aatgatgcac gccacccggc agctccaggg tggcggcacc
     1621 cggtatgagg aatggcaggg caatgaaaaa ttgcggcgga tcattgccgc ccgctccttc
     1681 tcctggggcg gaagcctgca tgaaaacgac ctggttacga cggcaggcgg gatgaatgcc
     1741 ctgtccttct gcatgatggc cctgggaaag cccggtgata cgatagcggt ggagagcccc
     1801 gtttacccgg gtattctgca actggccgta agcctgggtc tgaaggtttt ggagctgcct
     1861 acccatccaa acacaggtgt tgagatcgaa gcactgaaga aagcgcttcc ccggatcgat
     1921 ctctgcctcc tggtcaccaa cttcggtacg cccctgggaa gctgcatgcc cgatgaaaat
     1981 aagagagaag tagtggcgct gctggccaaa caggggatcc ctctcatcga agatgatctg
     2041 tacggcgatc tgcatttcgg agaccaacgg ccaaaatgtt gtaaggcatt tgacaaacag
     2101 ggaacggtgc tgtggtgcag ctccatttcc aaaaccctgg cgcccggata ccgtgtaggt
     2161 tgggtagcgc caggcatcta caaggaacag atcatgaaac taaaactgat ccatgccatc
     2221 tcctccccca ccataacgca ggaagtggtg gccggcttcc tgaaaagcgg ccgatacgag
     2281 aatcatcttc gaaagatgcg tcggatctta caggacaatt accaccatta tgccggtacc
     2341 atcgccggat attttccgga aggcaccaaa acaagcaggc cccagggtgg gcttgcctta
     2401 tgggtcgagc tcgacaaaaa gatcgatacc gcagagcttt acgatatcgc catcaaacac
     2461 aaaataagca ttgccccggg aaggatgttc accttgcagg atcaatttca aaactgtatg
     2521 cggctttgta ttggcttgcc atggtcggaa aacctgcagc ataaattgca actactgggt
     2581 agattggcga aaatgatccg gtaatgtcgg aatacccacc ataacttgtc ggaataacaa
     2641 cccctgcctt tcaaaaaaaa ccctcagctt tacagtatca cactaaaagc aaagcatatg
     2701 tcaaaagttt ctatttattt gaacttcgag ggcaacaccg aagaagcctt caactattac
     2761 aagtccgtgt tcaaaaccga atttaccgct cccctcatga gaatgaagga cgttccgccg
     2821 cagccgggaa tgcccccgct tcccgaaaaa gaggcgaacg ccgtcatgca cgtggcgctc
     2881 cccatccttg gtggaacgga actgcagggc accgacatgc tggaatccct cggccataag
     2941 ttgaagatcg ggaacaatac caccatctgc ctggagcccg acagcaagga agagaccgat
     3001 cgcatctata atgcgctgtc ggccggtggc tcggatacca tggctccgca ggatatgttc
     3061 tggggcgcgt attggggaac ctgtctcgac aagttcggca tccgctggat gtttaatttt
     3121 acctacaaga aataagaaag tcctcggact tcttccatct cccgtgatca gcggagggca
     3181 atacttcccc ctgctggtcg cggtgagcta tgaaacagct atacttttac ttcgtctgaa
     3241 gaaatgccat tacccaaata attataagaa gcatcgctgt ttgatatgcg ctccagaaag
     3301 cgcccatgcc attatcctta tcataatgca tcagactgac aaagatccct actatgggta
     3361 tccaatatat tagcttatta tgcataaacc aattactcct cccactcaaa gttacactaa
     3421 ttaccgatac cgatccgaag gaacgatagc ctccatggat ataaactgaa tgtagataga
     3481 tattatctat acaatcatca aaacaatcaa tttggccaat tgcctgcacc gcagtagctt
     3541 taactatcgc ccttgatagc cttcatccgg gaatatcaca ataaactcat ttcaatcaaa
     3601 agaaaatgga aacgctcaca tccacaagca aatgcccatt ccacaacgga acgctaaaac
     3661 ctggcgccgg cggcggcaca agaaacagcg actggtggcc caattccttg aaattgcaca
     3721 ttctccgtca acacgcttcg gaattcaacc cgatgggaga gaagttcgac tacgcaaaag
     3781 aattcaggac cctcgatctg aagggtgtaa aaaaggacat cttccaattg atgacgacct
     3841 cccaggattg gtggccggca gactacggtc attatggtcc ctttttcatc cggatggcct
     3901 ggcacagtgc gggtacctac cgcatccatg acggccgggg tggcgcaggc gccggtatgc
     3961 agcgctttgc tccgttgaat agctggccgg acaatgccaa tctcgacaaa gcccggttgc
     4021 tgctgtggcc ggtgaaaaag aagtatggaa ggaagatctc ctgggcagac ctgatgatcc
     4081 tcgccggcaa ctgcgccctc gagtcgatgg gcctcaagac ctttggtttt gccggcggac
     4141 gcgcggatgt ctgggagccg caggaagacg tctattgggg gtctgaaacg gagtggctgg
     4201 gcgacaaacg ctacaccggc gaccgtgtac tggagaatcc gcttggcgcc gtccagatgg
     4261 gcctgatcta tgtcaatccg caagggccca acgggaagcc cgatcccctg gccgcggccc
     4321 gcgacatccg cgagaccttc cgccggatgg ccatgaacga ttatgaaacc gtggccctca
     4381 tcgcaggtgg ccacaccttc ggtaagaccc atggggccgc cgatgccgac aaatacgtcg
     4441 gccgggaacc cgcggcagcc accctggaag agcagggcct tggctggaaa aacagcttcg
     4501 gcaagggcaa tgcagatgat acgatcacca gcggtctcga gggcgcatgg accacgaccc
     4561 cgacaaaatg gagcaacaac ttcttcgaaa acctgttcgg cttcgaatgg gagctcacca
     4621 aaagccccgc cggtgcacat caatggaaac caaaagccgg cgccggcgcc aataccgtgc
     4681 ccgatgcgca cgatccgtca aagcgtcacg caccaacgat gcttaccacc gatctctccc
     4741 tgcgcatcga cccggcctac gagaaggttt cccggcattt ctatgaacat ccggatgagt
     4801 tcgcagacgc ctttgcccgc gcctggtaca agctcaccca tcgtgatatg gggccgaagg
     4861 cccgttatct tgggccggaa gtgcctgccg aagagctgct ctggcaggac ccggtcccgg
     4921 ccgttaccca tcccctcatc gacgcaaaag atatcgctgc gctgaaggat aaggtattgt
     4981 cgagcgggct gtccgtgtcg caactggtat ctactgcctg ggcctcggcc gctactttcc
     5041 gggggtcgga taagcgcggc ggcgccaacg gcgcccgcat ccggctggct ccccaaaagt
     5101 actgggaggt aaacaaccca ccccagctgt ccaaagtatt ggatacgttg agcggcatcc
     5161 aacaggaatt caataaagcc caatccggtg gcaagaaagt ttcgctggcc gacctgatcg
     5221 tgctggccgg ctgcgccggg gtggagaaag cggcgaagaa tgcggggctg gacgtgcagg
     5281 cgccctttac gcccggtcgt gtcgacgctt cacaggagca gacggatgta gagtcattcg
     5341 ccgttcttga accggctgcc gacggtttcc gcaactacct tggcgccgat gctcctgcct
     5401 cggcggagga aatgctcatc gacaaggcgc aactgctgac actgaccgcg cccgagatga
     5461 cggtcctcat cggcggcatg cgggtgctga acacgaattt cgatcaatcc gcccatgggg
     5521 tccttaccaa taccccggaa gcgctgacca acgatttctt cctgaacctg ctcgatatgg
     5581 gcaccacctg gaaggccgcc tccgaaacca tgcaggtgtt cgaaggacgc gaccgggctt
     5641 ccggtaagat aaaatggaca ggcacccgtg tcgatctcat ctttggttcc aactccgaac
     5701 tgcgcgccct tgccgaagtg tacggatgcg aagactccaa agaaaaattt gtacaggact
     5761 ttttggccgc atggaataaa gtgatggatc ttgaccgttt cgacctggtc tgatctcccg
     5821 ctccgcgagt cataaatgaa tgcccccggt gaaccggggg cattttcgtg gaaccctatt
     5881 atcgcttaat cgtcttgcca atagttgcca atcccccttg cacgacccgc acgatataca
     5941 tcccctgcgc cagcccatta agccccgtca atctgaacac attttccccc ttattcgcgg
     6001 tatattcccg gcgcatcagg ataacaccgg ccatgtcccg gagctgtacc tccacaggac
     6061 cacctgccgg taaggtacag tcgatctcca tatcgcttcc aaaagggttg gggacgatcc
     6121 tgtccagctg cggaacggcc gcaccatcac cggacatcct caccaacaca acgcccgagt
     6181 agctgtaccg tccatcttta tccaccagcg ccaaccggta ataattatca cggggatagc
     6241 cgatgggatg catatccgta aagctgtagg atgcaagatt agcgctattg cctgccgcgc
     6301 tcacctggcc gatcgtttcc cagtcaagac catcggcgct gcgcccgatc tcaaaccgaa
     6361 ggttaccggc ccccggcggg gtcgtccagt taagcagcac agtagtattt acctgcctgc
     6421 cggtgaagga ggcaagtccg acgggcagta caaccatcgt attggacacg atgtaaatgc
     6481 cggttccccc ggaaaaactg gtaaccccat cgaactcggc atagttgacc ccgtcttttg
     6541 ttccatacac aggcgcaagc ataatcgtcg cgccctttac attggccttg ggtgtggctg
     6601 tcaggctgtc gggggtaaag gcattgccga ctgttttaaa ccactcgaga ttgctcatca
     6661 acgccgcccc gctgctggtc ttgaaggcct tcgccgcatt tcgggctgcc agcgtatcgg
     6721 cggggttgaa aaagaaacgg acattgaccg ggctggtcag gggggtgccg gtatacacat
     6781 tccagtag
//
LOCUS       DATGFG010000086         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1355016, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000086 DATGFG010000000
VERSION     DATGFG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355016"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1199)
                     /locus_tag="VKQ52_01630"
     CDS             complement(78..1199)
                     /locus_tag="VKQ52_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ85903.1"
                     /translation="MRTLRFLLQKELRQMRRDPAIWRILFLMPVIQLLILPLAADYEV
                     KNINLSVTDHDRSVYSQLLVRKVIASGYFRLTDYSPGFGTALRAVERDKADLILEIPP
                     RWDRDLIKDGSATLFIAVNAINGVKAGLGAAYLQGIIQDYNAEVRAKWLAPARMNPQP
                     VIEVQYSDWFNPHMNYRFFMVPGILVMLLTMIGSSFASNNIVREIELGTIEQINVSPI
                     RKVHFILGKLIPFWLLSMLILTVGLILARVVFGIVPLGHYATIYLFAGVYLLSILGLG
                     LLMSTYAQTQQQSMLVSFFITMIFNLMSGLYTPVESMPEWGQWIARLTPVSYFIQVMR
                     MVVLKGSGVADIVPHILAVLGFAVVFNGWAILNYKKRRG"
     gene            complement(1196..2302)
                     /locus_tag="VKQ52_01635"
     CDS             complement(1196..2302)
                     /locus_tag="VKQ52_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ85904.1"
                     /translation="MKQLLVFVRKEWYHIWRDRRTLLILIGMPIIQIVLYGFALTNEV
                     KNSNIAVLDAAQDASSIPLVQEIAASRYFHLLRYIHSPRDIEGVFRKGKIRMAIVIPR
                     DFRNDLLHTNHAQVQLIADASDPNTANTLTNYAVAIINTYQSSLNVDQSLPYSIHTQL
                     RMLYNPELKGAYNFVPGVMAMVLMLVCAMMTSISIVKEKEMGTMEVILVSPLKPFRIV
                     IAKTIPYLLISFVNIVTILLLSVYALDVPIAGSLVLLVAESLLFTGLSLSIGILISNS
                     TRSQQTAMFTSMMSLFLPTLIFSGFLFPVENMPVPLQVIANVVPAKWYFIIVKDVMIK
                     GAGFEAVWKETLVLAGMMTVLLGISIRNFKIRLT"
     gene            complement(2305..3045)
                     /locus_tag="VKQ52_01640"
     CDS             complement(2305..3045)
                     /locus_tag="VKQ52_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ85905.1"
                     /translation="MSETVISAKALTKRFAGFTAVDAISFDVRKGEIFGFLGANGAGK
                     TTAMRMLCGLSLPSSGEARVAGYDVYRQTELIKRNIGYMSQKFSLYEDLTVKENIEFY
                     GGIYRLSDARIREKIRELLEQLQLGDQANSLVRSLPLGWKQKLAFSIAIVHDPAIVFL
                     DEPTGGVDPIMRKEFWGMIYAAADRGITVFVTTHYMDEAEYCHRVSIMVDGRIEALDD
                     PGTLIRQHGAASMDEVFLQLARKATRKE"
     gene            complement(3042..>3482)
                     /locus_tag="VKQ52_01645"
     CDS             complement(3042..>3482)
                     /locus_tag="VKQ52_01645"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLZ85906.1"
                     /translation="TVMSEAKPLTRSVAQIEDQLRRAMVLNPVSGVVLTKYAMAGEVT
                     TPGKALYKIANLSVITLRVYVTGTQLSGLKIGQHLRVFADDGPVRYREYEGVITNIAD
                     KAEFTPKTIQTKEERADLVYAVKVHVKNDGYLKIGMYGEVKFTK"
BASE COUNT          822 a         1078 c          858 g          724 t
ORIGIN      
        1 acccggccgg cagcggcgga aatatgacct ttatcagggg aacaactgac tcttgagaag
       61 agaaggactg ccggcgatca gccccggcgt ttcttgtaat tgaggatcgc ccagccgttg
      121 aacactaccg cgaacccaag cacggcaagg atatgcggaa cgatatccgc caccccgctg
      181 cccttcagca ccaccatcct catcacctgt ataaaatagc tcaccggcgt cagccgcgcg
      241 atccactgcc cccattcggg catgctttcc accggggtgt acaagccact catcaggttg
      301 aagatcatag taatgaaaaa ggataccagc atcgactgct gctgcgtctg cgcataggtg
      361 gacatcagca gacccaaccc gagtatcgac aaaaggtaaa cacccgcgaa caaatagatc
      421 gtcgcatagt gacccagcgg tacgatcccg aataccaccc gcgcaaggat caacccgacc
      481 gtcaggatca gcatggacaa cagccagaag gggatcagct tcccaagaat gaaatgtacc
      541 ttccgtatcg ggctcacatt gatctgctcg atagtgccca attcgatctc ccggacgata
      601 ttattggacg cgaacgaaga cccgatcatc gtcaataaca tcaccaggat ccccgggacc
      661 ataaaaaacc ggtaattcat atgcgggttg aaccagtcgc tgtattgcac ctcgatcacc
      721 ggctgcgggt tcatcctcgc cggcgcaagc catttcgccc gcacctccgc attgtaatcc
      781 tggatgatgc cttgcaaata ggccgctccg agaccggcct tcaccccatt gatcgcattg
      841 acggcgataa acagcgtggc cgacccgtcc ttgataagat cacggtccca ccgcggcggg
      901 atctccagta taagatcggc cttgtcccgt tccaccgccc gcaaagccgt cccgaaaccg
      961 ggactgtagt ccgtgagccg gaaataaccc gaagcaatga ccttccgcac cagtaattgg
     1021 gaatacactg aacggtcgtg atccgttacc gaaagattga tgttctttac ctcgtagtcc
     1081 gcggcaaggg gaaggatcaa cagctggatc acaggcatca ggaaaaggat ccgccagatc
     1141 gccgggtcgc gccgcatctg gcgcaattcc ttctgcagca aaaaccggag cgttctcatg
     1201 tcagcctgat tttaaagttt cggatgctga tcccgagcaa cacggtcatc atccccgcca
     1261 gcaccagcgt ctccttccag accgcttcga accccgcacc cttgatcatg acatccttga
     1321 caatgataaa ataccatttg gcaggcacca cgttggcgat cacctgcagc ggcaccggca
     1381 tgttctccac cgggaacaga aacccgctga agataagcgt cggcagaaaa agactcatca
     1441 tcgaggtgaa catcgccgtc tgctggctcc gcgtggaatt ggagatcagg atgccgatgg
     1501 aaagggacaa acccgtgaac aacaggctct cggctaccag cagtacgagg ctgccggcaa
     1561 tcggcacgtc gagcgcatag acgctcagca gcaggatagt cacgatattg acaaaagaga
     1621 tcagcaggta tgggatcgtc ttggcgatga cgatacgaaa aggcttcaac ggcgatacga
     1681 ggatcacttc catcgtgccc atctcctttt cctttacgat cgaaatggac gtcatcatgg
     1741 cgcacaccag catcagcacc atcgccataa cgcccggtac aaaattgtag gcccctttca
     1801 gctccgggtt gtagagcatc cgcaactgcg tatggatcga atagggaagg gactggtcca
     1861 cgttcaggga cgactggtag gtattgatga tggcaaccgc gtaattggtc agggtattgg
     1921 ccgtattggg gtccgaagca tccgcgatca actgcacctg cgcatggttc gtatgcagca
     1981 ggtcgttcct gaaatcgcgc gggattacaa tggccattcg gatcttcccc ttccggaaca
     2041 ccccctcgat gtcgcgtgga ctgtggatat atcgcagcag gtgaaagtac cggctcgcgg
     2101 caatctcctg caccagcggg atcgacgagg catcctgggc cgcatccagc acggcgatat
     2161 tgctgttctt cacctcgttg gtcagggcaa acccgtaaag cacgatctgg atgatgggca
     2221 tgccgataag gatcagcaag gtccgccggt cgcgccagat atgataccac tcttttctta
     2281 cgaataccag cagctgtttc atgatcattc ttttcttgtt gccttcctgg caagttgaag
     2341 aaatacttca tccatcgaag ccgccccatg ttgccggatc agcgtcccgg ggtcgtcgag
     2401 ggcctcgatc ctcccgtcca ccatgatcga tacccggtgg caatactccg cctcatccat
     2461 atagtgtgtc gtaacaaaga ccgtgatccc ccggtcggca gcggcataga tcatccccca
     2521 gaattccttg cgcatgatcg ggtcgacacc ccccgtcggc tcatcgagaa aaacgatcgc
     2581 cggatcatgc acgatagcga tcgaaaaagc cagcttctgc ttccaaccca acggcaggct
     2641 ccgcaccagg ctgttcgcct ggtcccccag ttgcaactgt tccaacaatt cacggatctt
     2701 ttcccggatg cgcgcatcac tcaaccggta gatcccacca tagaattcaa tgttctcctt
     2761 cacggtgagg tcttcgtaca gggagaactt ctggctcata tacccgatat ttcgcttgat
     2821 caactcagtc tggcgataaa catcataccc ggcgaccctc gcctcccccg aactgggcaa
     2881 ggacaggccg cagagcatgc gcatggccgt cgtctttccc gccccattgg cgccgaggaa
     2941 cccgaagatc tcccccttcc gcacatcgaa ggaaatagca tccaccgccg tgaacccggc
     3001 aaatcgcttg gtcaatgctt ttgccgatat gaccgtctca ctcattttgt gaattttact
     3061 tccccgtaca tccctatttt gagatacccg tcattcttca catgaacctt gacggcatag
     3121 acaagatccg cccgctcctc cttcgtctgg atggtcttgg gcgtgaactc ggccttgtcc
     3181 gcgatattcg tgataactcc ctcatactcc cggtaacgca ccggaccatc gtcggcgaat
     3241 acgcggagat gctgcccgat cttcagcccg gacaactgcg ttcccgtgac atatacccgt
     3301 aacgtgatca ccgacagatt ggctatctta tacaaggcct tccccggcgt cgtcacctcg
     3361 ccggccatgg catatttggt cagcaccacc ccgctcaccg ggttcagcac catcgcccgc
     3421 cgtaactggt cctctatctg ggccaccgat cgggtcaggg gcttcgcttc actcatgacc
     3481 gt
//
LOCUS       DATGFG010000087         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_558068, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000087 DATGFG010000000
VERSION     DATGFG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558068"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VKQ52_01650"
     CDS             <1..232
                     /locus_tag="VKQ52_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLZ85907.1"
                     /translation="PTGAGDTFAGGFIGHLANTRDISFENMKTAIIVGSAMASFCVEK
                     FGPTRLKEITRADIDTRIRQFVDLVNFDIELV"
     gene            444..1436
                     /locus_tag="VKQ52_01655"
     CDS             444..1436
                     /locus_tag="VKQ52_01655"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HLZ85908.1"
                     /translation="MKVAVVGATGLVGTKMLQVLAERNFPVTELLPVASEKSIGKQVE
                     FKGKKYTVVSANDAIAAKPALALFSAGGSTSLEWAPKFAAAGITVVDNSSAWRMDPSK
                     KLVVPELNADALTPADKIIANPNCSTIQMVVALNPLHKKYKIKRIVVSTYQSVTGTGV
                     KAVTQLMNERKGVSGEMAYKYPIDLNAIPQIDVFLDNGYTKEEMKMVHETKKIMRDDT
                     IRVTATTVRIPVMGGHSEAVNVEFENEFDLAEVRSLLSKAPGVVVVDDPSTQQYPMPK
                     DAHERDEVFVGRLRRDETQAKTLNMWVVSDNLRKGAATNAVQIAEYLNANKMLG"
     gene            complement(1478..2140)
                     /locus_tag="VKQ52_01660"
     CDS             complement(1478..2140)
                     /locus_tag="VKQ52_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HLZ85909.1"
                     /translation="MLQVSTLKFLKDLKKNNNKPWFEKNKSVYLDAKEDMDHFVEQVI
                     DGFGKTDPEIATLHPKDCTYRIYRDVRFSKDKTPYKIHMGAYLNKGGKKVPTAGYYLH
                     LEPGKNMAGGGLWMPQPPELNKVRQEIDYNFSEWTKILGDRNFKKVFPGGLEMEEMLS
                     RPPKGYEDDNPAIEYLKLKSFIITRDFSDAEVQSKTFLKEIIKTFTAMKGFVYFMNLS
                     LE"
     gene            complement(2185..2748)
                     /locus_tag="VKQ52_01665"
     CDS             complement(2185..2748)
                     /locus_tag="VKQ52_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HLZ85910.1"
                     /translation="MIRKLTGEVWKPLQFPGWKQLRKKYALSSMGRVASYSEDVLADG
                     KLLNGSLTTGYKTLNLHRPGNNGTLYIHREIARLFLPKAAPKNKYVIHVNHNKLDNAS
                     RNLKWASLEEMIDHQQKSPAKIAYKKVQANRTVGLKLTANQVKSIKKTLSSKNRQLTI
                     KKLAEKYNVSEMTMYRIKSGENWGRIK"
     gene            complement(2814..>3152)
                     /locus_tag="VKQ52_01670"
     CDS             complement(2814..>3152)
                     /locus_tag="VKQ52_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HLZ85911.1"
                     /translation="SVYDFFHNTDNLHLLKDLESLGLNVQSQKSKASSGGNLDGQTFL
                     FTGTLSRLKRSDAEAAVETNGGKLLSGVSSKLNYLIVGEDAGSKLEKAKKIPSIQILT
                     EDEFIKLLNI"
BASE COUNT          738 a          833 c          782 g          799 t
ORIGIN      
        1 tcccacgggc gccggcgaca cctttgccgg tgggtttatc gggcacctcg ccaatacccg
       61 cgatatctcc tttgaaaata tgaagacggc gatcatcgtc ggatcggcca tggcctcctt
      121 ctgcgtggag aagtttggtc ccacccgcct gaaagagatc acccgcgccg atatcgacac
      181 ccggatcagg caattcgtcg acctggtcaa ctttgatata gaactggttt aagccgcgcc
      241 ttcgacccaa aaacatttgt tttatttacc gttgatctct atatttgccc cgcaatgcaa
      301 ataatgaaac atacaaagca gattaagaag agcctcccac agcgggacag gatctgatcg
      361 ttttgtatgt caagatatca ggagccttcc cgcaacggga aggctctttt gttttgcgaa
      421 catttataac caaaataata tcaatgaaag ttgcagtcgt cggcgccacc ggactggtcg
      481 gcaccaaaat gttacaggtc ttggcagaga ggaatttccc cgtcaccgaa ttgctccccg
      541 tagcatccga gaaatccatc gggaagcagg tcgagttcaa ggggaagaaa tataccgtcg
      601 ttagcgccaa tgatgcgatt gcagcaaagc cggcattagc cctcttttcg gccggcggca
      661 gcacctccct cgaatgggcg ccaaagttcg ccgccgccgg tatcacggtc gtggacaatt
      721 ccagcgcatg gcgcatggac cccagtaaaa agctggtcgt tcccgaactc aacgccgatg
      781 cccttacgcc cgcggacaag atcatcgcca accccaactg ctctactatc cagatggtag
      841 tggcgctgaa ccccctgcac aaaaagtata aaataaaacg gatcgtggtc tccacctacc
      901 agagcgtgac cggaacgggc gtgaaggccg tcacccaact gatgaacgaa cggaagggcg
      961 tcagcggcga aatggcgtat aaatatccca tcgacctgaa tgccatcccc cagatcgacg
     1021 tcttcctgga caacggctat acaaaggaag aaatgaagat ggtccatgag acaaagaaga
     1081 tcatgcgcga cgataccatc cgcgtgacgg caaccaccgt ccgcatcccc gtcatgggcg
     1141 gccacagcga ggcggtgaac gtggaattcg agaacgagtt cgatctggcg gaggtccgct
     1201 ccctgctgag caaggcgccc ggcgtggtcg tcgtcgacga cccctccacc cagcaatacc
     1261 ccatgccaaa agatgcgcac gaacgggacg aggtcttcgt aggacgactc agaagggacg
     1321 agacccaggc aaagaccctc aatatgtggg tagtaagcga caatctccgg aaaggcgccg
     1381 ccaccaacgc ggtgcagatc gccgagtacc tgaacgccaa caaaatgctt ggataaaaaa
     1441 aaggcccccg gtttccccgg gggcctctta ctatgcttta ctcgagactt agattcataa
     1501 aataaacaaa gcccttcatt gccgtaaacg tctttattat ttctttcagg aaggtttttg
     1561 actggacttc ggcgtccgaa aaatcccggg taatgatgaa acttttcaat ttgaggtact
     1621 cgatggcggg attatcgtct tcatacccct tgggcggtcg cgaaagcatc tcctccattt
     1681 caaggccccc gggaaacacc ttcttgaaat tccggtcgcc aaggatcttc gtccattcgg
     1741 aaaaattata gtctatctcc tgccggacct tgttcaattc cggaggctgg ggcatccaca
     1801 aaccgccgcc tgccatgttc ttacccggct ccaggtgcag gtaataaccg gcggtaggta
     1861 ctttcttgcc gcccttgttg aggtaagctc ccatgtggat cttgtagggt gttttgtcct
     1921 tggagaagcg gacatcccgg tagatgcggt aggtacaatc ctttggatgt agggttgcta
     1981 tttcaggatc tgtctttcca aacccatcga tcacctgctc tacaaaatgg tccatgtcct
     2041 cttttgcatc caggtaaaca gacttgttct tttcaaacca gggcttattg ttgttcttct
     2101 taaggtcttt aaggaatttg agcgtggaga cttgcagcat agtttcggtt tctctgtctt
     2161 tgttccagtc tgacgtcgga cggtctattt gatcctgccc cagttttcgc cactcttgat
     2221 ccgatacatg gtcatttcgc tgacattgta cttttcagcc agtttcttga tggtaagctg
     2281 tcggttcttg ctggataagg tttttttgat ggacttcacc tggttggcgg tgagcttaag
     2341 tccaacggta cggttggcct gaactttttt gtacgcgatc ttcgcaggac tcttctgctg
     2401 gtgatcgatc atctcttcca gggatgccca cttcagattc ctggaagcat tgtccagttt
     2461 gttgtgattc acatggatca catacttgtt cttgggagct gcctttggca ggaaaagccg
     2521 ggcgatctct cgatggatat agagagtgcc gttgttgccg ggccggtgaa gattcagggt
     2581 cttgtaaccg gtcgtaaggc tgccgttcag caatttgccg tcggctagca catcttccga
     2641 gtagctggca acgcgaccca tggaagaaag tgcgtatttt tttcggagtt gtttccagcc
     2701 gggaaactgt aaaggtttcc acacttcgcc ggtgagtttt ctaatcatgc ctgtcaattt
     2761 tcttaaagtt acgaaaaaga atttcgaatt accagtaacg taagaaaata ttattatatg
     2821 tttaggagct tgataaactc gtcctctgtg agtatttgaa ttgacggtat ctttttggcc
     2881 ttttccagct tgctgcctgc atcctccccg acgatcaggt agttcaactt actgctgaca
     2941 ccgctcagta gcttccctcc gttggtctcc accgccgctt ccgcgtcgga tcgcttcaac
     3001 cggctaaggg tgccggtgaa taagaacgtt tgcccatcca ggttgccccc gctgcttgcc
     3061 ttggatttct ggctctgtac attcaggcca agggattcca ggtccttcaa caggtgcagg
     3121 ttatcggtat tatgaaagaa atcatacacg ct
//
LOCUS       DATGFG010000088         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1468367, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000088 DATGFG010000000
VERSION     DATGFG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468367"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1411)
                     /locus_tag="VKQ52_01675"
     CDS             complement(50..1411)
                     /locus_tag="VKQ52_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HLZ85912.1"
                     /translation="MNYISTMDWYVITNPETLDTPALIVFPERVRANIRLAIEMVGDP
                     ARLRPHVKTHKSPDLTRLQLAAGITRFKCATIAEAEMLALEGAPDVLLAYQPIGPKVE
                     RFATLIRKYPKTAFSCLIDNPDAAAAMAKTFAAANLTVPVYLDINVGMDRTGIAPGDE
                     AIQLYRMATQLDGIDPVGLHAYDGHIRDTDLAERTRKCDEAFAKVVALRKNIAALANT
                     PHPAPASAAASPTAPPASASPTASARSGLSLASPASSSASGAPTAAASPPTKPAASPT
                     ASATSASPTSLKIIAGGSPTFPVHARRPAIAEGGAQTEPESVQCSPGTFVYWDKGYGD
                     AFPDQGFQPAALVVARVISLRGDTRLCLDLGHKSIAPENEISRRVFFLNGPELKPVGQ
                     SEEHLVVEAGPGHNYRIGDIFYGVPFHICPTVAQYDHAYTVESGRVTGAWKNIARDRV
                     LTI"
     gene            complement(1398..1868)
                     /locus_tag="VKQ52_01680"
     CDS             complement(1398..1868)
                     /locus_tag="VKQ52_01680"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLZ85913.1"
                     /translation="MSNHTPEQNLAALNLTLPPAPAPLGVYKPCLIDGKYLYVSGHGP
                     LRDDKTLITGRVGQDLDADQGKLAARQVGLAILATVKGHIGSLDKVKRVIKTLGMVGC
                     TSDFERHPFVINGCSELFVQVWGPDNGVGVRSAVGMGSLPGNIPVEIEVIFELH"
BASE COUNT          386 a          501 c          765 g          460 t
ORIGIN      
        1 gttagccgag gggggcaagt tggggtttgt ttttgagggt acggggggat cagatggtga
       61 gaacgcggtc gcgggcgatg ttcttccacg cgccggtgac gcggccggat tcgacggtgt
      121 atgcatggtc gtattgggcg acggtcgggc agatgtggaa gggtactcca tagaagatat
      181 cgccgatccg atagttgtgg cccgggccgg cttcaaccac caggtgttct tcgctttggc
      241 cgacgggttt cagttcgggg ccgttgagga agaagacgcg acgcgagatc tcgttctcgg
      301 gagcgatgga tttatgaccg aggtcgaggc agagccgggt gtcgccgcgt aaggagatga
      361 cgcgggctac gacgagggcg gcgggttgga agccctggtc ggggaaggcg tcaccgtagc
      421 ctttgtccca gtagacgaag gttccgggac tgcattgcac agactcgggc tccgtctgcg
      481 ccccaccctc ggcgattgcg gggcgcctgg catgcaccgg gaaggtgggg gagccgccag
      541 caatgatctt tagcgatgtg ggtgaggcag acgtggcgga ggcagttggt gaggcagcgg
      601 gtttggtggg tggcgatgcg gcagcagttg gcgctccgga ggcagatgac gatgcgggtg
      661 aggcaagaga taacccagac ctggcggaag cagttggtga ggctgaggcg ggcggcgcag
      721 ttggcgatgc ggcggcggat gccggggcgg gatggggtgt attggcgagg gccgcgatgt
      781 ttttgcgcag ggccacgact tttgcgaagg cttcgtcaca tttgcgggtg cgttcggcga
      841 ggtcggtatc acggatatgc ccatcgtatg cgtggaggcc gaccgggtcg atgccgtcaa
      901 gttgggtcgc catccggtag agttggatcg cctcgtcacc gggtgctata ccggtgcggt
      961 ccatccccac atttatatca agatataccg gaacggtaag gttcgccgcc gcgaaggttt
     1021 ttgccatggc ggctgcggcg tcggggttgt cgatgagaca ggagaatgcg gttttgggat
     1081 attttcttat gagcgtcgcg aagcgttcta ctttcgggcc gatgggttgg taggcaagca
     1141 aaacgtcggg tgcgccttcg agggcgagca tttcggcttc cgcgatggtg gcgcatttga
     1201 agcgggtgat gcccgcggcc agctggagac gggtgaggtc ggggcttttg tgcgtcttga
     1261 cgtgtggccg cagacgcgcc ggatcaccga ccatctcgat ggccaggcgg atgtttgccc
     1321 ttactctttc cgggaagacg ataagcgcgg gggtgtccag cgtttcggga tttgttatga
     1381 cataccaatc catggtgcta atgtagttca aaaataactt ctatttccac gggaatattg
     1441 ccggggaggg agcccatgcc gacggcgctg cgcaccccta ccccattgtc gggtccccag
     1501 acctggacga agagttcgct gcagccattg atgacgaaag ggtggcgttc gaagtcggaa
     1561 gtgcagccga ccatgccgag ggtttttatc acgcgtttta ccttatcgag actgccgatg
     1621 tggcctttga cggtagcgag gatggcaaga ccgacctgac gggccgcgag ctttccctgg
     1681 tcggcatcga gatcctgtcc gacgcggccg gtgatcagcg ttttgtcgtc gcgtagcggg
     1741 ccatgaccgg acacatacag gtacttacca tcgatgagac agggcttata cacgccgagc
     1801 ggagccggtg cgggtggaag ggttaggttg agggcggcga ggttttgttc gggtgtgtgg
     1861 ttgctcattt tttcttattg tcgcttgtgc agaaaatgga agcgaatgta gtatttttag
     1921 tcaatctaac gacaatgcta taattatgaa aaagacaacc cgcagaaatt ttatcggccg
     1981 cagtgcgctt gcgcttggcg cggcgtttgg ccttggacag ttgcctgccc ttgcaagggc
     2041 atcgatggct gacgccggtg gccgacggga aggtccggga gacatcggca ataccggtct
     2101 ccgaagagaa gg
//
LOCUS       DATGFG010000089         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1369727, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000089 DATGFG010000000
VERSION     DATGFG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369727"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1332
                     /locus_tag="VKQ52_01685"
     CDS             196..1332
                     /locus_tag="VKQ52_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ85914.1"
                     /translation="MRNSLQWIGFMSVAVSLSLVSCGDSKPAGPQAPPPTPVDVYTVA
                     SGKATYFDSYPGTATPLNQVDIKPQVSGNIIGIYFKDGQEVKKGQRLYEIDQQQYKAA
                     VEAAEANVDVARANLAKAQQDADRYQELSKQDAIAKQTLDHQIADLEASKRQLAAAQA
                     NVASVATNLKYSVIFSPFNGTIGISQVKVGTSVYPQTLLNSVSTDDPMAVDIAIDQAE
                     IPKFTRFFGKSVKPKDSLFTAVLPDGTIYSYPGSLYLLDRSVDPTTGTLKARITFPNP
                     KNELKAGLTTNIRVKHDAGDSSLLIPYKAVVDQLGESFVFIIENGHALQRKVTLGTKI
                     ADKIVVKSGVKAGEQVVINGVQKLRDSSAVAVGAPKAPAGSTGK"
     gene            1368..>2985
                     /locus_tag="VKQ52_01690"
     CDS             1368..>2985
                     /locus_tag="VKQ52_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ85915.1"
                     /translation="MKSSNIFLRRPVTAIVISLVILLVGGLAILNMPISQYPDITPPT
                     VQITGNFVGADAQTVEQTTTTAIETQVNGVPGIAYMQSNSANNGTSQITATLNIGADP
                     NIVALDIQNRVGIATPTLPQQVQKLGITTLKRNPAILMLVAVYSPHHTHSIPFMDNYA
                     NIYLRDILLRTPGVGDVFSRADNFSMRVWLKTDKLAQLGLTANDVINAVNEQNIQVAA
                     GSTGAPPQNNAQAFEFTIFTNSRLTTEQQFGDIVVRSNPQQNSIVYLKDVARITLGKV
                     SYAANSFVDGDRSSYLLVYQAPGSNALATAEGVYKALADAKKYFPADLNYVVPFESVS
                     VVKVSIADVVQTLFIALGLVTLVVFLFLQNWRSTLIPVLAIPVAIIGTFIFFIPLGFT
                     INTLTMFGFVLAIGIVVDDAIVVVEAVQHYIDTEHLSPKEATARAIADISGPVIAIAL
                     ILAAVFVPVGFIPGIVGRLYQQFAITIAISVLISAFVALSLTPALCMLLLKEHRVGKK
                     ARGLNHLFYLFNNWFDRFSLRYTIGVKGWIK"
BASE COUNT          697 a          887 c          783 g          618 t
ORIGIN      
        1 ttcaatacca tcgagctgca gtaccgctcg ggtgtcaagg cctatctcga actgatcacg
       61 gccgaaacgg atctgcgtac ggcgcagtcc aattacagca atgcgctcta cctcgtgctg
      121 agcagcaagc tggatgttca aaaagcattg ggtagcataa cttattaatc acacttggat
      181 aaattatctt tttatatgag aaatagctta caatggattg gttttatgtc ggtcgccgtt
      241 tctttgagcc ttgtctcctg cggagacagt aaacccgccg ggccgcaggc gcccccgccc
      301 acgccggtag acgtctacac cgtcgcttca ggcaaggcca cgtacttcga ttcctacccc
      361 ggtacggcaa cacccctgaa ccaggtggat atcaaaccgc aggtgtccgg caatatcatc
      421 ggcatctatt ttaaggatgg gcaggaagta aagaaaggac agcggctgta tgagatagat
      481 cagcaacaat ataaggccgc cgtggaggct gccgaggcca atgtggatgt agccagggcc
      541 aacctggcca aggcgcagca ggatgcggac cgttaccagg aattgtccaa acaggacgcc
      601 atcgccaaac agaccctcga ccatcaaata gccgatctcg aagccagcaa gcgccagttg
      661 gcggctgccc aggccaatgt ggccagcgtg gcgaccaacc ttaaatactc ggtgatcttt
      721 tcccccttca acggaaccat cggcatctcg caggtaaagg tcgggacatc cgtttacccg
      781 caaaccctgt tgaactcggt gagcaccgat gaccccatgg ccgtcgatat cgccatcgac
      841 caggcggaga tcccgaaatt cacgcgcttc tttggaaaga gtgtaaagcc aaaggactcg
      901 ctctttacgg ccgtattgcc cgatgggacg atctacagct accctggttc gctctattta
      961 ctggacaggt cggtcgatcc aaccacggga acactgaagg caaggatcac cttccccaat
     1021 ccgaagaatg aactcaaggc cggcttgacc actaacatcc gggtaaaaca tgatgccggc
     1081 gacagcagcc tgctcatccc ttacaaggcc gtcgttgacc agctggggga aagctttgtg
     1141 ttcatcatcg agaatggtca cgcgctgcaa cgcaaggtta cgctgggcac caagatagcc
     1201 gataagatag tagtcaaaag cggagtcaag gccggcgaac aggtggtcat caatggggtg
     1261 caaaagcttc gcgacagcag cgctgtagcg gtgggtgcgc ccaaagcacc cgcgggcagt
     1321 acaggaaagt aagataagag aggatctaac tcaaaaaata acttggaatg aagtcttcaa
     1381 atatatttct caggaggccg gtgacagcca tcgttatctc gctggtgatc ctgctggtgg
     1441 gaggcctggc tatcctgaac atgccgatca gccaatatcc ggatattact ccgccgaccg
     1501 tacagatcac cggcaacttc gtcggcgcag atgcccagac ggtggaacag acgacgacca
     1561 cagcgatcga gacccaggtg aatggggtgc cagggatcgc ctatatgcag agcaacagcg
     1621 ccaacaacgg cacctcgcag atcacagcaa ccctcaacat tggagccgac ccgaatatcg
     1681 tggccctgga tattcaaaac cgggtgggta ttgccactcc gacgctgccg cagcaggttc
     1741 agaaactcgg gatcaccacc ctcaagcgga accctgccat cctcatgctg gtggctgttt
     1801 attcgccaca ccatacgcat agcatcccct ttatggacaa ctatgccaat atctatctgc
     1861 gggatatcct ccttcgtaca ccaggggtag gggacgtgtt cagccgcgcc gataacttca
     1921 gtatgcgcgt ctggctcaag accgataagc tggcgcagct gggtctcacc gccaacgacg
     1981 tgatcaacgc cgtgaatgaa cagaacatcc aggtggccgc cggttccacc ggtgcgccgc
     2041 cgcagaacaa cgctcaggcc ttcgagttca ccatattcac caacagccgg ctgaccacgg
     2101 aacagcagtt cggcgatatc gtggtccgga gcaaccccca acagaactcc atcgtgtacc
     2161 tcaaggatgt ggcccggatc acactcggta aggtgagcta tgctgccaac tccttcgtgg
     2221 acggcgaccg ctcttcctat ctcctggtgt accaggctcc gggcagcaac gcccttgcca
     2281 cggccgaggg ggtatataag gccctcgcgg acgcaaagaa atacttcccc gcagacttaa
     2341 actacgtggt tcccttcgag tcggtatccg tcgtgaaggt ttccatcgcc gatgttgtcc
     2401 aaaccctctt catcgccctt ggactggtta ccctggtcgt gttcctcttc ctccagaact
     2461 ggcggtccac actgatcccg gtgctcgcga tccccgtagc gatcatcgga accttcatct
     2521 tcttcatccc cctcggcttc accatcaata ccctgacgat gttcggtttt gtgctggcca
     2581 tcgggatcgt ggtggatgac gccatcgtgg ttgtggaggc cgtgcagcac tatatcgata
     2641 cggaacatct gtccccaaaa gaagcgacag cccgggccat cgccgatatt tccggccccg
     2701 ttatcgccat tgcgctgatc ctggcggccg tgttcgttcc cgtaggattt atcccgggta
     2761 tcgtcggccg gctctaccag cagtttgcca tcaccatcgc catctcggtg ctgatctcgg
     2821 cattcgtggc gctctccctg acacccgcgc tctgtatgct gctgttgaag gagcaccggg
     2881 tgggaaaaaa ggcccggggg ctcaaccacc ttttctatct cttcaacaac tggttcgacc
     2941 gtttcagtct ccggtatacc atcggggtca agggctggat caagc
//
LOCUS       DATGFG010000090         6987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1353699, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000090 DATGFG010000000
VERSION     DATGFG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353699"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1463)
                     /locus_tag="VKQ52_01695"
     CDS             complement(54..1463)
                     /locus_tag="VKQ52_01695"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ85916.1"
                     /translation="MNRKSFLRNSSMAALALAASPAANALPNETTHTTPQKNTPAKTT
                     PLHFAGPKVRMALIGTGLRGQGHLGLLLRRDDVDLVAICDIDDYMLTSAKNAITRSGK
                     KMPQVYTGNPYAWQDLLKQQQLDGVIIATPWEWHKPMIIGAIEAGVKYVATEVILGIT
                     LQDHWDVVKAAEKYNANVMMLENVCYRRDVMAVLNMVRQGLFGEIVHLQGGYQHNLQG
                     IKFNNGQPNGGAGCEFGEKAFSEARWRTEHSVHRNGDLYPTHGIGPLAHYVEINRGNV
                     FTTLCSFSTKSRGLHDYVVRHCGENHPSAKVKFKLGDIVTTSINCANGETILLQHDTN
                     LPRPYSLGFRVQGTNGLWMDVAKGIYVEGQSKEDEWDPAKTWLDKYDHPLWKRWNNEA
                     EGAGHGGMDFFVIHAFVESIKRKTPTPQDVYDAAAWSAITPLSEQSIELGNATVPFPD
                     FTGGQWMYRKPTFALNDEY"
     gene            1597..2106
                     /locus_tag="VKQ52_01700"
     CDS             1597..2106
                     /locus_tag="VKQ52_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85917.1"
                     /translation="MERQDMGVREVKSGISVVAVIERLALREFRTIRASRSFGFDWMQ
                     YRSLEVYKLKILPDNTIAGLICLIDHSVEAENAIEIECLEVSKENVGASKRFDGIAGS
                     LIAFACRESFKRGHEGFVFLRPKTGLIQHYTEAYGFRHFPLKTVSRPEGIMVADGRAA
                     ANLIKLFLE"
     gene            2117..2644
                     /locus_tag="VKQ52_01705"
     CDS             2117..2644
                     /locus_tag="VKQ52_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85918.1"
                     /translation="MRPAKKSERKSVAEEIKEALVAPGSLKGEGRKEMLESFRAIRKK
                     ITEQYTVEDEFVTRFLQLKFLIEDYVSSDVFSDRYFFGYFLKEYISRQEKKNKEFAWE
                     IDIDPAELSLVINRHRRPTDKLIFRLDIHSNKNFPAQLWFKVLEKDRAIELLQDSSII
                     DREKKHVKRKLSFSF"
     gene            complement(2641..3102)
                     /locus_tag="VKQ52_01710"
     CDS             complement(2641..3102)
                     /locus_tag="VKQ52_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ85919.1"
                     /translation="MASLIIVRPATTADIPTLLRFEQSVIAAERPFDPTLKENPVYYD
                     LPALIASPDTQLLVAELDGAIIGSGYARIEKANPMYKHSHHAYIGFMYVDPGHRGKGI
                     NQLIIDGLRQWAHGRGITELRLEVYYDNEAAIRAYEKAGFTRLWIQMRTPV"
     gene            complement(3128..4051)
                     /locus_tag="VKQ52_01715"
     CDS             complement(3128..4051)
                     /locus_tag="VKQ52_01715"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ85920.1"
                     /translation="MPINHPLQPIALTPYGHLQTLTENRRIFNLRNCELNVFETNETS
                     YSVPLTFGDFVITSMVRGKKIMHLAGKPAFDYLPGETVIVPANETMVIDFPDACSDAP
                     TQCIALAVDSAYIRDTVDYLNDHYNDTDEAHNWQLHFDQYHFHNDTDVTELIDKLIRI
                     CTSANAGKNIFADLSLKELLIRILQSQNLLLQSEEAGKSSNSSRLHFVLQYIHEHLTE
                     RIPVDTLSRKAYLSRNIFFKWFKEQFGLTPLEYITRERIRLARRLLADEKNTIGQVSL
                     QCGFSDVNYFVRAFKKSEGLTPGAYQDICRK"
     gene            4184..5719
                     /locus_tag="VKQ52_01720"
     CDS             4184..5719
                     /locus_tag="VKQ52_01720"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLZ85921.1"
                     /translation="MSTSATAAAPVSNLAVRPSFKEKYDHFIGGKWVAPDSGEYFDNI
                     SPIDGKVFTQAARGNARDIEKAIDAAHTAFPLWSKTPAAYRSNLLLKIAQIVEENLEK
                     LAVIETIDNGKPIRETRAADLPLVIDHFRYYAGVIRAEEGTVSELDETTVSINIHEPI
                     GVVGQIIPWNFPLLMATWKMAPALAAGCCVVMKPAEQTPTSIMCLMELIGDILPPGVL
                     NIVTGFGPEAGKPLASSPRVQKVAFTGETTTGRLIMQYASENLIPVTMELGGKSPNIF
                     FESVADADDEFLDKAVEGAVMFALNQGEVCTCPSRILVHEKIYDQFMNRVIQRTKAVK
                     MGNPLDPTVMMGAQASEDQYNKILSYLEIGKQEGAQVLCGGEAFHQNSGLEHGFYIQP
                     TLFKGNNKMRIFQEEIFGPVSCVTTFGSTEEAIAIANDTLYGLGAGVWTRDAHELYTV
                     PRAIQAGRVWGNCYHAYPAHAPFGGYKKSGFGRETHKMMLNHYRQTKNMLISYSKAKL
                     GFF"
     gene            5719..6093
                     /locus_tag="VKQ52_01725"
     CDS             5719..6093
                     /locus_tag="VKQ52_01725"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HLZ85922.1"
                     /translation="MSVPRIISTGKAEELIDRLRQMHGPLMFHQSGGCCDGSQPMCFA
                     DGEFRTGGSDVCLGEVYGCRFFMSLDQFEYWKHTQLILDVTPGRGSSFSLEIPLGVRF
                     LIRSRVFTEEELGSVFPVTKHP"
     gene            complement(6064..6477)
                     /locus_tag="VKQ52_01730"
     CDS             complement(6064..6477)
                     /locus_tag="VKQ52_01730"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ85923.1"
                     /translation="MSTQLHAYLNFNGRCREAMTFYQQCLGGDLVLQKIAESPMAAQM
                     PSEAGANILHSSLTRNGVLLMMGSDMMGANLKQGNSVVLCLDCSSDEEINTFFKKLSA
                     GGEIKMPLHQSFWGPTYGELTDKFGMLWMFSYRKN"
     gene            complement(6493..6927)
                     /locus_tag="VKQ52_01735"
     CDS             complement(6493..6927)
                     /locus_tag="VKQ52_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85924.1"
                     /translation="METYHLDHDIRLCCIPAKSFPDGVMAAFQQIMDIFPDQQGKRRL
                     FGVSWPDGKGSLVYKAAVEESYKGEAEKLGMDTYLLKSGEYLSTVVHNFMDDIPSIGN
                     AFRQLVEAPGVHPNTVGVEEYVSTTDVRCMVPMFMETAPSHN"
BASE COUNT         1474 a         1564 c         2214 g         1735 t
ORIGIN      
        1 aaaggccccc gggaatgccc gggagcctga tgccggatgg acggaaatct tgcttaatat
       61 tcgtcgttca gggcgaaagt cggtttgcgg tacatccact ggcccccggt gaagtcgggg
      121 aaggggacgg ttgcgttacc caattcaatg gactgttcgc tgagcggggt gatggcgctc
      181 catgccgcgg cgtcgtagac gtcctgtggt gtcggggtct ttcgttttat agattcgaca
      241 aaggcgtgga tgacgaagaa atccattccg ccatgaccgg cgccttccgc ttcgttgttc
      301 cagcgtttcc acagcgggtg atcgtatttg tccagccagg tctttgcagg atcccattca
      361 tcttcctttg actggccttc cacgtagata ccttttgcaa catccatcca aaggccgttg
      421 gtaccctgga cgcggaagcc gagggagtag ggccggggca ggttggtgtc gtgttgcagg
      481 aggatggtct cgccgttggc gcaatttatc gaggtggtga cgatgtcgcc taatttgaat
      541 tttactttag cgcttgggtg gttctcgccg cagtgtctga cgacgtagtc gtggaggccg
      601 cgggattttg tcgagaagga gcagagggtg gtaaagacat taccgcgatt gatctccaca
      661 taatgcgcca gggggccgat gccgtgtgtc ggatagagat cgccgtttct gtgaacggag
      721 tgttccgttc gccagcgggc ttcggagaag gccttctcgc cgaactcgca gccggcgccg
      781 ccattgggct ggccgttatt gaattttatt ccctggaggt tgtgctgata gccgccctgg
      841 aggtggacga tctcgccgaa cagcccctgc cgcaccatat tgagaacggc catgacatcc
      901 cgccggtagc agacgttctc cagcatcatg acattcgcgt tgtatttttc cgctgctttt
      961 acgacatccc aatgatcctg cagggtgatg ccgaggatga cttcggtggc aacatatttc
     1021 acgccggctt ctatggcgcc gatgatcatg ggtttatgcc attcccaggg agtggcgatg
     1081 atgacgccgt ccagttgttg ttgcttgagc agatcctgcc aggcgtaggg attgccggtg
     1141 tagacctggg gcattttttt gccggatctt gtgatggcgt tctttgcgct ggtgagcatg
     1201 tagtcgtcga tatcgcagat ggcaacgagg tctacgtcgt cgcggcggag caggagaccg
     1261 agatgtccct ggccgcggag accggtgccg atgagggcca tcctgacctt tggacccgcg
     1321 aagtggaggg gagtagtttt tgcgggggta ttcttttgtg gggtggtgtg ggttgtttcg
     1381 ttggggagtg cgttagcggc ggggcttgcc gcgagggcga gggctgccat gctgctgttg
     1441 cggagaaatg attttctatt catggtgggc ggctgttttc atcaaactta aaggattcgg
     1501 gagggatttg ctggcgcaaa cgattgctta aaagtggatt ttgccatttt caatattggc
     1561 aaataatttt acaaatttgt aaattttcgt atcttagtgg aaagacagga tatgggtgtg
     1621 cgagaggtga agagcgggat atccgtagtg gcggttatcg aaagattggc attgagggag
     1681 tttaggacca tccgtgcgag ccggtcattc gggtttgatt ggatgcaata tcgatccctg
     1741 gaggtatata agctgaagat attaccggat aatacaattg cggggttgat ctgcctgatc
     1801 gaccattcgg tcgaggccga aaatgcgata gagatcgaat gccttgaggt gagtaaggag
     1861 aatgttgggg cgagcaagcg atttgatggg attgccggtt cccttattgc ctttgcctgc
     1921 cgggagtcgt ttaaacgggg acatgaggga tttgtgtttt taagacctaa gacgggttta
     1981 atacaacatt ataccgaggc gtatggcttt cgacattttc ctttgaagac ggtttcccgg
     2041 ccggagggaa taatggtggc ggatgggcgg gcggctgcaa atttgataaa actattctta
     2101 gaataaattg gagtgtatga gaccagcaaa aaagagcgaa cgcaagagtg ttgcggagga
     2161 gataaaggag gcactggtgg cgcccgggtc cctgaagggg gaggggagga aggaaatgtt
     2221 ggaatcgttc agggcgataa ggaaaaagat cacggagcaa tataccgttg aagatgaatt
     2281 tgttaccagg ttcttacaat tgaagttcct gatcgaggat tatgtatcat ccgatgtctt
     2341 ttccgaccgt tatttttttg gttatttttt aaaggaatat atctcccggc aggaaaagaa
     2401 aaataaggag tttgcctggg agatcgatat tgatcccgcc gagttgagct tggttatcaa
     2461 ccggcataga aggccaaccg ataaattgat ctttcggttg gatattcatt cgaataagaa
     2521 ttttcctgct caattgtggt ttaaagtgct tgagaaggat cgggcaatcg agttattgca
     2581 agacagcagt atcatcgatc gcgagaagaa gcatgtgaag cggaaactaa gcttttcgtt
     2641 ttagacgggg gttcgcattt ggatccagag gcgggtgaag ccggcttttt cgtaggcgcg
     2701 gatggcggct tcgttgtcgt agtagacttc gaggcggagc tcggtgatgc cgcggccatg
     2761 agcccattgg cgaaggccgt cgatgatgag ctggttaatg cctttgccgc ggtgaccggg
     2821 atcgacatac atgaagccga tataggcgtg gtgggaatgt ttgtacatcg ggttggcctt
     2881 ttcgatgcgc gcgtagccgg agccgatgat ggcgccgtcg agttcggcga cgaggagttg
     2941 ggtatcgggt gaggcgatga gggcgggaag atcgtagtag acagggttct ccttcagggt
     3001 ggggtcgaag ggtctttccg cggcgatgac gctttgttcg aagcgaagga gtgtggggat
     3061 atcggccgtg gttgccggcc ggacgatgat gagtgatgcc atccgtaaag atagtgataa
     3121 ccgcgggtta tttgcggcag atgtcctgat aggcgccggg ggtgaggcct tcggattttt
     3181 tgaaggcgcg gacgaaataa tttacatcgc tgaagccgca ttgaaggctt acctggccga
     3241 tggtgttctt ttcatcggcc agcaggcggc gggcgaggcg gatgcgttcg cgggtgatgt
     3301 attccagtgg tgtcaggccg aattgttcct taaaccattt aaagaagatg ttacggctga
     3361 ggtaggcctt gcggctgagc gtgtccacgg ggatacgttc ggtgaggtgt tcgtggatgt
     3421 attgcagcac gaagtgcagg cggctgctgt tgctgctctt gccggcctct tcggactgca
     3481 gcagcaggtt ctggctttgg aggatgcgga tgagcaattc cttcaggctc agatcggcga
     3541 agatgttctt gccggcgttg gcgctggtgc agatgcgaat gagcttgtcg atcagctccg
     3601 tcacatcggt gtcgttgtgg aaatgatatt gatcgaagtg tagttgccag ttgtgggcct
     3661 cgtccgtatc gttgtagtga tcattgagat aatcaaccgt atcacggata taggcgctgt
     3721 ccaccgcgag ggcgatgcat tgggtgggag catccgagca ggcgtcggga aagtcgatga
     3781 ccatggtctc gtttgcgggg acgatgacgg tctctcccgg cagatagtcg aatgcgggtt
     3841 ttccggccag atgcatgatc ttttttcccc ggaccatact ggtgatgacg aagtcgccga
     3901 aggtgagggg gacactatag ctggtctcgt tggtttcgaa gacgttgagc tcgcagttcc
     3961 gcaggttgaa gatacgccgg ttctccgtca gggtctggag atgcccatag ggagtgaggg
     4021 caatcggctg gagtggatgg ttgatcggca tggcaagcgg cggttgagat acaagttacc
     4081 gattttggta cgattgtgct ataaataaat acaaaagttt gtgccctacc gtggttttgg
     4141 gtggaatttt aagcattcaa tcacgcctaa aattgcttta gccatgagta cgtctgcaac
     4201 ggcggctgcc ccggtgtcca acctggcagt acggccttcc tttaaagaaa aatacgatca
     4261 ttttatcggt ggtaaatggg tggcacccga cagcggggaa tatttcgata atatctcccc
     4321 gatcgacggc aaggtcttta cgcaggctgc ccggggcaat gcccgggaca tcgagaaggc
     4381 catcgatgcg gcccatacgg catttccgct gtggagcaag actcccgcgg cataccggag
     4441 taatttgctg ttgaagatag cgcagatcgt cgaggagaac ctggagaaac ttgcggtcat
     4501 cgagacgatc gacaacggca aaccgattcg tgagacccgg gccgccgatc tgccgctcgt
     4561 gatcgatcat ttccggtatt atgcgggagt gatccgtgcc gaagaaggga cggtcagtga
     4621 gcttgatgaa acgacggtga gtatcaatat ccacgagccg atcggggtag ttggacagat
     4681 catcccctgg aacttccccc tgctgatggc tacctggaag atggcgccgg ccctggcggc
     4741 gggttgttgt gtggtgatga agccggccga gcagacgccc accagcatca tgtgcctgat
     4801 ggagctcatt ggggatattt tgccgcccgg ggtgctgaat atcgtgacgg gctttgggcc
     4861 tgaggcgggc aaaccccttg cttcctcgcc ccgtgtacag aaggtggcct ttaccggcga
     4921 gactactacg ggcaggctga tcatgcaata tgcgtccgag aacctcatcc ccgtgacgat
     4981 ggaactgggt ggcaagagtc ccaatatctt cttcgagtcg gtggccgatg cggatgatga
     5041 attccttgac aaggccgtgg aaggcgcagt gatgttcgcg ctcaaccagg gcgaggtttg
     5101 cacctgtccc agccggatcc ttgtacatga gaagatctat gaccagttca tgaacagggt
     5161 catccagcgg accaaggccg tcaagatggg taacccgctc gaccccacgg tgatgatggg
     5221 tgcgcaggct tcggaggatc aatacaataa gatccttagc tacctggaga tcggcaagca
     5281 ggaaggcgcc caggtgttat gcgggggcga ggccttccac cagaacagcg ggctggaaca
     5341 tggtttttat atccaaccga cgctgttcaa ggggaataac aagatgcgca tcttccagga
     5401 agagatcttt gggccggtga gctgtgtcac cacctttggg tcgacggaag aggcgatcgc
     5461 gattgccaac gatactttat atgggcttgg cgcaggggta tggacccggg atgcgcatga
     5521 gctgtatacc gttccgagag cgatccaggc gggtcgggtc tggggcaact gttaccacgc
     5581 ctatccggcg catgcgccat tcggcggata caaaaaatcg ggatttggcc gggaaacgca
     5641 caagatgatg ctgaaccatt accggcagac caagaacatg ttgatctcct atagtaaagc
     5701 caaattaggt ttcttctaat gtcagttccc cgtatcatat ctaccgggaa ggcggaggaa
     5761 ttgatcgacc ggttgcggca aatgcatggc cctttgatgt tccatcagag cggcggatgt
     5821 tgtgacggat cgcaaccgat gtgttttgcg gacggcgaat tcagaaccgg cggcagtgat
     5881 gtgtgccttg gtgaggtcta tggctgccgg ttctttatgt cgctggatca attcgaatac
     5941 tggaagcata cgcaactgat cctggatgtc acccctggcc ggggcagcag tttttccctg
     6001 gagatcccgc ttggggtccg tttcctgatc cgcagccggg tgttcacgga agaagagctg
     6061 gggtcagttt ttcctgtaac taaacatcca tagcattccg aacttatccg tcagttcgcc
     6121 gtaggtgggg ccccagaagc tttgatgcag gggcattttt atttccccgc cggcggaaag
     6181 ttttttgaag aaggtgttta tctcttcgtc gctgctgcag tcgaggcaga gtacgacgga
     6241 atttccctgt ttcagattgg cgcccatcat gtcggagccc atcatcagca ggaccccgtt
     6301 acgggtaagg ctgctgtgga ggatattggc cccggcttcg gagggcatct gtgccgccat
     6361 gggcgactcg gctatttttt gcaggaccag gtcgccaccg aggcattgct ggtaaaaggt
     6421 catcgcttcg cggcagcggc cgttgaaatt gaggtaggcg tggagttgtg tcgacatggc
     6481 catgcgcttt tattagttat gggaaggcgc cgtctccatg aacatgggga ccatgcagcg
     6541 gacgtcggtg gtgcttacat attcttccac gccgacggta ttgggatgga cgccaggggc
     6601 ttcgaccaat tgccggaagg cattgccgat ggaggggatg tcatccatga aattgtgtac
     6661 cacggtgctc aggtattcgc cacttttcag gaggtaggtg tccattccta atttttcggc
     6721 ttctccttta tagctttctt cgacggcggc tttgtagacg aggctgcctt ttccgtccgg
     6781 ccaggagaca ccgaacaggc gccgtttgcc ctgttgatcg gggaagatgt ccataatctg
     6841 ctgaaacgcg gccattacgc cgtcggggaa gctctttgcc gggatgcagc agagacggat
     6901 gtcgtggtca aggtgatagg tttccattac cggttgattt ttataagaca aagctaaatt
     6961 ctctttacgg ctttggaaat gtgtgaa
//
LOCUS       DATGFG010000091         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_98882, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000091 DATGFG010000000
VERSION     DATGFG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98882"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="VKQ52_01740"
     CDS             <1..2085
                     /locus_tag="VKQ52_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLZ85925.1"
                     /translation="PGDVGQSLEKITAAIKAKKLRWKDIDARVKKVLYAKYRYGLAHW
                     QPVSKVHLTADLNAGITEMRRRVAVDAMTVLRAEPGVFPLRASAEAANPAGMQDLVGT
                     GLSVARKKEKKIAYIGMGLAEDNAFSRRMRANYNAHVYFFDYDLDSVKAAAALELLSN
                     RYEAIVIGMHGYARFPANNFAISRPAAWLLHDLQQKEKAITFFFGNPYAIRNSCDART
                     IVACYEDDSVTQTVAADLLNGKFTARGHLPVTVCEEWKAGAGVIGPERLLPVVDPVKL
                     GMDTAKLQKIDAICQEAIDKKATPGCVVLVAKDGKIAYEKAFGYMTYDQTEPVYPETI
                     YDMASCTKICATTMAVMKLYDEGRLDVQKTLGDYLPWVKGSDKDSLKLWDILLHQAGL
                     VPDVVFYKEIVDSSVAQDPLPGYFTPIPDPVHGVRVAEKMYLRTDWLDTMNLRIVQSK
                     LLPRKYIYSDNDFIFLGKVVEAITGMTLDQYVKKTYYDPLGMTVAGFRPRERFPLGRI
                     APTADEPIFRHQLIRGDVHDPGAAMFGGVAGHAGLFSDAYDLALLEQMLLNGGTMNGI
                     SFLKPSTIGFFTAYHSDISRRALGFDKPEKDNAHRKEPYPCLSASPETFGHTGFTGTC
                     VWVDPKYDLIYIFLSNRVNPVENNPRLSGLSIRGRIQETVYQSMGVDMGASTKEPAPP
                     EKKKKRKKCCAM"
     gene            complement(2086..2844)
                     /gene="nth"
                     /locus_tag="VKQ52_01745"
     CDS             complement(2086..2844)
                     /gene="nth"
                     /locus_tag="VKQ52_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HLZ85926.1"
                     /translation="MTRKQRFQFVLDYFSKHNPDAATELLYDSPYQLLVAVILSAQCT
                     DKRVNMTTPAIFQQYPDAASLARASFDELFPYIRSISYPNNKTKHLIGMAQMLMTKFG
                     GEVPLTVEELVQLPGVGRKTANVITSVVDNQPNMAVDTHVFRVAARIGLTVNAKTPLA
                     TEKQLLEFIPREMVHVAHHWLILHGRYVCVARNPKCPECGLQQACKYFQKNFGTPAAD
                     TTRLPVLKTRARATAKSGPAVKAATTPSKRATKR"
     gene            complement(2904..3143)
                     /locus_tag="VKQ52_01750"
     CDS             complement(2904..3143)
                     /locus_tag="VKQ52_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85927.1"
                     /translation="MTLFTPEDLLRYLYKESSPELTVAIESALKEDWMLREKLEVLRS
                     SVTELDTVIVSPRMEVIQRVMNYARKTAVQETVQH"
     gene            complement(3323..>3554)
                     /locus_tag="VKQ52_01755"
     CDS             complement(3323..>3554)
                     /locus_tag="VKQ52_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HLZ85928.1"
                     /translation="DSRDLITSHFRERAELQKLERSKKYYEQQIAATKQELEQLKTNP
                     AVLEKYAREKYLMKRDNEDLFVIRDDNAPAKH"
BASE COUNT          753 a          842 c         1131 g          828 t
ORIGIN      
        1 cccggggatg taggtcaaag cctggagaag ataacggcgg cgataaaagc gaaaaaactt
       61 cgttggaagg atattgatgc ccgggtgaaa aaagtgctct atgccaaata ccggtatggt
      121 cttgcgcatt ggcaacctgt cagcaaggtg catcttaccg ccgatctcaa tgcgggcatc
      181 acggagatgc ggcgccgggt ggcggtggat gcgatgaccg tgctgcgggc ggaaccgggt
      241 gtcttcccgc tgcgggcgag cgcggaagcg gccaatcccg caggcatgca ggacctcgtg
      301 ggtacgggtt tgtcggtggc caggaagaaa gagaagaaga tcgcctatat cgggatgggc
      361 ctggcggagg acaatgcctt tagccgccgc atgcgggcca actataatgc ccatgtgtat
      421 ttttttgact acgatctcga cagcgtcaag gctgccgcgg cgctggagct gctgagcaac
      481 cgctatgagg cgatcgtgat cggcatgcat ggctatgccc gatttcccgc gaacaacttc
      541 gccatcagcc ggcctgccgc ctggctgctg cacgacctgc agcaaaagga aaaggcgatc
      601 actttctttt ttgggaatcc ctatgcgatc aggaacagtt gcgatgcgcg gacgatcgtc
      661 gcctgttatg aggatgattc ggtgacgcag accgttgccg ccgatctgct gaatggaaaa
      721 tttaccgccc gggggcatct tcccgtgacg gtgtgtgaag aatggaaggc cggtgcgggt
      781 gtgatcgggc ccgaacggct gctgcccgtg gtcgacccgg tgaagctggg gatggatacg
      841 gcaaagctgc agaagataga tgccatctgc caggaggcga tcgacaagaa agcgacgccg
      901 ggttgtgtgg tattggtggc aaaggacggc aagatcgcct atgaaaaggc ctttggctat
      961 atgacctatg accagacgga gcctgtctat cccgagacga tctatgacat ggcgtcctgt
     1021 acaaagatct gtgcgacgac gatggcggtg atgaagctct atgacgaggg ccgtctcgat
     1081 gtgcagaaga cgcttggcga ctatctgccc tgggtgaagg gcagtgataa ggactcgtta
     1141 aagctctggg atatcctgct gcaccaggcg gggcttgttc cggatgtcgt cttttataag
     1201 gagatagtcg acagcagtgt ggcgcaggat ccgttgcccg ggtattttac gcccatcccc
     1261 gacccggtgc atggtgtgcg ggtggccgag aagatgtatc tgcgtaccga ctggctggat
     1321 acgatgaacc tgcggatcgt ccagagcaag ctgctgccga gaaaatatat ctacagcgac
     1381 aacgatttta ttttcctggg gaaggtggtg gaagcgatca ccgggatgac gctggaccag
     1441 tatgtaaaaa agacctatta tgatccgttg gggatgacgg ttgcgggctt caggccccgg
     1501 gagcggttcc cgctggggcg gatcgcgccg acggccgatg agcctatctt ccggcatcag
     1561 cttatccgcg gggatgtaca tgatcccggt gcggcgatgt tcggcggtgt cgccggtcat
     1621 gccgggctct tcagcgacgc ctatgatctg gcgctgctgg agcagatgct gctcaatggg
     1681 gggacgatga atgggatcag cttcctgaaa ccttcgacca tcggcttctt tacggcgtat
     1741 cacagtgata tcagccggcg cgcactgggt ttcgacaagc ctgagaagga caatgcgcat
     1801 cgcaaggaac cctatccctg tctttcggca tcgccggaga cctttgggca taccgggttt
     1861 acgggcacct gtgtatgggt ggacccgaaa tatgatctta tctatatctt cctttccaac
     1921 cgggtgaatc cggtagagaa caatccgcgt ctttcggggc taagtatccg cgggcggatc
     1981 caggagacgg tctatcagtc gatgggggtg gatatggggg cttcgacaaa ggagccggcg
     2041 ccgccggaga agaagaagaa gcggaagaaa tgctgcgcga tgtagtcatc tttttgttgc
     2101 gcgtttggag ggcgttgtgg cggctttgac ggcgggcccg gatttggctg ttgcgcgcgc
     2161 gcgcgtcttc aggacgggga ggcgggtggt atcggctgcg ggtgtgccga agtttttctg
     2221 aaagtatttg caggcctgct gcagtccgca ttcggggcat ttggggttgc gggcgacgca
     2281 gacatagcgt ccgtggagga tgagccagtg gtgggcgacg tgcaccattt cgcgggggat
     2341 gaattccagc agttgttttt cggtggcgag gggggtcttt gcgttgacgg taaggccgat
     2401 gcgggccgct acgcggaaga catgcgtatc gacggccata ttgggctggt tgtcgacaac
     2461 ggaggtgatg acgttggctg ttttgcggcc gacgcccggc agctggacca gctcttcgac
     2521 ggtgaggggg acctcgccac caaatttggt catgagcatt tgcgccatgc cgatgaggtg
     2581 tttcgtcttg ttgttgggat aggagatgct cctgatgtag gggaataatt cgtcgaagct
     2641 ggccctggcc agtgatgcgg catccgggta ctgttggaaa atagccggag tggtcatgtt
     2701 gacccgttta tccgtgcatt gtgcggacag gatcaccgct accagcaatt ggtaggggct
     2761 gtcgtacaat agctcagtgg ctgcatcagg gttatgctta ctgaaatagt ccagtacgaa
     2821 ctgaaaccgt tgcttcctag tcattagagt cggtcattgg aaatgatcgt aatgaccgcg
     2881 aatgtacggg aaatggcgca gggttagtgc tgtacagtct cctgcactgc cgttttcctg
     2941 gcatagttca taacgcgctg gataacttcc atgcgggggg agacgataac cgtatctaat
     3001 tctgtgaccg aagatcgcaa aacctccaat ttttcacgca gcatccagtc ttcctttagc
     3061 gcagactcta tcgctaccgt taattccggg gaagactctt tatacaaata ccgcaaaaga
     3121 tcctccggtg taaagagtgt cataggcaaa accatttaat gtcctaataa atatccgaaa
     3181 gcagtaaaag tgaaacgtcc gttatgaaaa cggtatttgg gtactgttat tgtccggtaa
     3241 agcaattatt aacttttccc ggggcctgac ggggcaagcg cttagctata aagggggcga
     3301 gaattgtgta aacgggtgaa aatcaatgtt tggcaggcgc gttgtcatca cggatcacaa
     3361 agagatcctc attgtctctt ttcataagat atttctcccg ggcatatttt tcgagtacgg
     3421 ccgggttggt cttcagctgt tcgagttcct gtttggtggc ggcgatctgc tgttcgtagt
     3481 atttcttgct tctctcgagt ttttgcagtt cggcccgctc ccggaaatgg ctcgtgatca
     3541 gatcccggga gtcg
//
LOCUS       DATGFG010000092         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1010922, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000092 DATGFG010000000
VERSION     DATGFG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010922"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VKQ52_01760"
     CDS             <1..238
                     /locus_tag="VKQ52_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85929.1"
                     /translation="KFTPLPSYGSYFQLYKYDRISDEPDKDFAIRITKEYGVASIPVS
                     AFYQSGKDDRVVRFCFAKKAETLEKAAERLQKIK"
     gene            244..852
                     /locus_tag="VKQ52_01765"
     CDS             244..852
                     /locus_tag="VKQ52_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HLZ85930.1"
                     /translation="MKIDTLVFDLGGVLIDWNPEYLYNKIIPDEKERRWFLSTICTPD
                     WNEQQDAGRSLQEATDLLVTKYPEHEKAIRAYYGRWKEMLGGPIHETVEIFRELKTRG
                     RLKLYALTNWSAETFPVALELYDFLHWFDGRLVSGEEKVRKPFPEIYKLLMDRFAIDP
                     QHAIYVDDNIRNVLPARELGFVGIHFRTPALFREELKKLGVL"
     gene            complement(877..>3044)
                     /gene="feoB"
                     /locus_tag="VKQ52_01770"
     CDS             complement(877..>3044)
                     /gene="feoB"
                     /locus_tag="VKQ52_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HLZ85931.1"
                     /translation="PLTFAPAIGRCSHPSLKKNTLHIALVGNPNSGKSSLFNALTGLN
                     QKVGNFPGVTVDKKTGVSELAPGLSVNIVDLPGTYSLYPKRGDEWVTYKVLLNQDPAV
                     KADMVVLLVDASNLKRNLLFCSQIIDLKIPVVVALTMMDLAAQKGIEIDVDGLERELG
                     IPVVPVNPRKNKGIPQLKKIVELRARQLSMAPPRAFIGNQALATAAISDVKKLFPEIS
                     DYTAIHYLINHEHFDFTEDLQTRIEAIELAHSFNHTRIQADEILQRYGRIKQIMQHTV
                     SEPDPLEKTLFTERLDKLFLDRTWGYIILLGVLFLLFQSVFWIAQFPMDAIDWTFSQL
                     GGWLGHFLPEAWWSDLLINGLLAGLSGILVFVPQIMILFGLITVLEDTGYMARISFLT
                     DKLMRKVGLNGKSVMPMISGFACAVPAIMSARNIENRKERLLTILITPLMSCSARLPV
                     YTMLIALVIPRKEVLGFFSLQGLVMMGLYMLGLLMAMIVSYVAKWFIHLKEKSFFILE
                     LPIYRAPRWNNVVVTMINKAKIFVVDAGKVIMVISLILWALSSFGPGRRSEVAAQYEQ
                     QIQQHPDQAPALKKEKQAALLENSYAGRLGKVVEPVIRPLGYDWKIGIALITSFAARE
                     VFVGTMATLYSVEGGKDADQQTLRQRMQSARREDGTPVYTLASGISLMIFYVLAMQCM
                     STLAVVRRETRSWKWPGIQLAYMTGLAYIMSLIVYQLLK"
BASE COUNT          649 a          784 c          945 g          666 t
ORIGIN      
        1 gaaatttacg cccctaccct cctatggcag ctatttccag ttgtataaat acgaccggat
       61 cagcgacgaa cccgataagg atttcgccat ccggatcaca aaggaatatg gagtggcctc
      121 gatcccggtg tcggcctttt accagtcggg aaaggacgac cgggtggtcc gcttctgttt
      181 tgcgaagaag gcggagacgc tggaaaaggc ggcggaacga ctgcaaaaaa taaaatgata
      241 cgtatgaaga tagataccct tgtcttcgac ctgggcggcg tgctcatcga ctggaacccg
      301 gaatatctct ataacaagat catccccgac gaaaaggagc ggcgatggtt tctttcgacg
      361 atctgtaccc cggactggaa cgagcaacag gatgccggaa ggagcctgca ggaggctacg
      421 gacctgctgg tgacaaaata tcccgaacac gaaaaggcga tccgcgccta ctatggccgc
      481 tggaaggaga tgctgggcgg accgatccat gaaacggtcg agatcttccg ggagctgaag
      541 acccggggcc ggctgaagct atatgccctc accaactgga gcgcggagac cttccccgtg
      601 gcgctggagt tgtatgattt cctgcattgg tttgacggga ggctggtgtc gggagaggaa
      661 aaggtgcgga agcccttccc ggagatctat aaactactta tggaccggtt tgccatcgat
      721 ccccaacacg ctatttatgt cgacgataat atccgcaatg tgctgcccgc ccgggagctt
      781 gggtttgtcg ggatccactt ccggacaccg gcattgttca gggaagagtt gaagaagctg
      841 ggggtattgt gatcatgtgg gagatgccct gttttgctat ttcagcaact gatatacgat
      901 caggctcatt atataggcca gcccagtcat ataggcgagc tggatgcccg gccatttcca
      961 gctgcgggtc tcgcggcgga ccaccgcgag ggtgctcata cactgcatgg ccaatacgta
     1021 gaagatcatc aggctgatgc cgctggcaag ggtatatacc ggggtgccgt cctcccggcg
     1081 ggccgactgc atccgctggc gcagggtctg ctggtcggcg tccttgccgc cttcgacact
     1141 gtagagggtg gccatcgtgc cgacaaagac ttcccgggcg gcgaaggagg tgatcagggc
     1201 gataccgatc ttccagtcat aacccagggg gcggatgacg ggctccacta cttttcccag
     1261 cctgccggca taggaattct cgaggagggc ggcctgtttc tcctttttca gggcgggcgc
     1321 ctggtcggga tgctgttgga tctgttgttc atattgggcg gccacctctg aacggcggcc
     1381 ggggccgaag gagctcaggg cccagaggat caggctgatg accatgatca cctttccggc
     1441 gtccacgacg aagatctttg ccttgttgat catcgtgacg acgacattgt tccagcgggg
     1501 cgcccggtag atgggcagtt cgaggatgaa aaagcttttt tccttcagat gaatgaacca
     1561 tttggcgaca tagctgacga tcatcgccat cagcaatccc agcatataca ggcccatcat
     1621 caccagtccc tgcagactga aaaaacccag gacctccttg cgggggatga ccagcgcgat
     1681 caacatggta tagaccggca ggcgggcgct acagctcatc aatggggtga tgaggatggt
     1741 gagcagccgc tccttgcggt tctcgatgtt acgggcgctc atgatcgccg gaacggcgca
     1801 ggcgaagccg ctgatcatag gcatgacgct tttgccgttg agccccacct ttcgcatgag
     1861 tttatcggtg agaaagctga tccgggccat atagccggta tcttccagga cggtgatcag
     1921 cccgaaaagg atcatgatct gcgggacgaa caccagtatc ccgctgagac cggcaagcag
     1981 gccgttgatc agcaggtcgc tccaccaggc ttcgggaagg aaatggccca gccagccacc
     2041 cagctgcgag aaggtccagt cgatggcgtc catggggaac tgggcgatcc agaaaacgct
     2101 ttggaagagc aggaagagta ccccgagcag gatgatatat ccccaggttc tgtcgaggaa
     2161 cagcttgtcg agccgttcgg taaaaagggt cttttccagg ggatcgggtt cggataccgt
     2221 atgctgcatg atctgtttga tccggccgta gcgctgaagg atctcgtccg cctggatacg
     2281 ggtgtggtta aagctatggg ccagttcgat cgcttcgatc cgcgtctgga ggtcttcggt
     2341 aaagtcgaaa tgctcgtggt tgatcaggta gtggatggct gtgtagtcgc tgatctcggg
     2401 gaacagtttt tttacgtcgc taatggcggc ggtggcgagg gcctggttgc cgataaaggc
     2461 ccgtggcggg gccatgctga gttggcgggc ccgtaattcg acgatctttt tcagctgggg
     2521 gatgcccttg ttcttccgcg gattgacggg gactacgggg atgcccagtt cccgttcgag
     2581 cccgtcgaca tcgatctcga tgcctttttg cgccgccagg tccatcatcg tcagggcgac
     2641 cacgacgggt attttgagat cgatgatctg gctgcagaaa aggagattcc gtttgaggtt
     2701 gctggcgtcc acgagcagga cgaccatatc ggccttaacg gcggggtcct ggttgagcaa
     2761 gaccttgtag gtcacccatt catcgccccg ttttgggtag aggctgtagg ttcccggcag
     2821 gtcgacgata ttgacggaga gaccgggggc taattcactg acacccgttt ttttgtcaac
     2881 cgtaaccccg ggaaaattgc cgactttctg gttcaggccc gtgagggcgt tgaacaggga
     2941 acttttcccg ctgttggggt ttcctacgag cgcgatatgg agggtattct ttttcaaact
     3001 gggatggcta caccggccaa tggcgggggc aaaggtaagt ggat
//
LOCUS       DATGFG010000093         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_895931, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000093 DATGFG010000000
VERSION     DATGFG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895931"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="VKQ52_01775"
     CDS             complement(<1..1960)
                     /locus_tag="VKQ52_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85932.1"
                     /translation="MQFHQFRLKSRYFLWIFSGIFFFSGALKAQVNTVEFGKNRLQFK
                     KFKWQYYQTTNFNTYFNQGGQDLGKFVLQLAEKELPGIEKFVEYGLQRRANIVIYNTY
                     NDMEQSNIGLNLDWQTTGGITKLVNNKMVVYYNGDHNNLRIQIRQGIARILVENILFG
                     DDLGEFAANQALLDLPQWLTDGYIEYAAENWNTVLDDQLKSSMLSGRYKNFYQFAYER
                     PNLAGHAFWYYLADKYKKENVTYFLYLSRVYRNLNNASLRICKKKFKEVLKDFMAEEE
                     EKYEKDLRGRRNFPKGTVSVTEDINDHKDFFHFSPNPVPKSQTYAVVEYNRGKYTVTL
                     HDNYIFTKVLLKNGVRSLQEQINPHYPLLAWDPKGTRLACIYWDRGKTRLFVYDVVRK
                     YKLINQDLPDFQQVQDFKYMLNNNTLILSAVRNGQSDIYVYDIEKQSYRQITNDIYDD
                     LDATFIAFPGKTGIIYSSNRPSAHAVSKDTVLPSNYHYNIFLVDNWNDNEGKQVSQLT
                     YLKYGNARYPMQYNSNHFTFVSDENGIANRYAGFFHTQRAGLDTVYKIGDELLHNPMP
                     SDLDSTLKAYNRQQPDTSYVVSLSNDSAYVFPITNYQSSLVETKSAGDNGQVSEVRQE
                     GDIKFLYKLKVDDAALAKRNITARMTDYR"
     gene            2041..2679
                     /locus_tag="VKQ52_01780"
     CDS             2041..2679
                     /locus_tag="VKQ52_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLZ85933.1"
                     /translation="MLTVKSFAFNPVQENTYVVSDEKDACCIIDPGCYFGNERTALKE
                     YIEQQGLSPIFLLNTHCHLDHVFGNKFVHDTWDLPLHLHEKEVPVLAAAPEWGLDWGM
                     PFENYRGELIFLTPGRSVTLGDHRLDILFLPGHSPGSIGFYNAAQGFLIGGDVLFREG
                     IGRTDFPGGDHATLLESIHTQLWPLPDDTIVYPGHGEPTTIGWERAHNPFLK"
     gene            complement(2733..4271)
                     /locus_tag="VKQ52_01785"
     CDS             complement(2733..4271)
                     /locus_tag="VKQ52_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLZ85934.1"
                     /translation="MSSIKKLAGETVWYGVSSIVAKFIVQLLTPYLTAKFRGTPEFGQ
                     MSLVYAAISFINIGVLFGLDYAYFRYINKKETSGDLYSTLLLSLVSSTMLITTATILF
                     RGPIATVLDVGGHPGYITLSALMIAFDALSALPFARLRHEGRPRKYALIRISGIILYV
                     GLVFFFLSACPRLLKTHPNSIIALIYQPRYGYVGYVLLANVIQNIFQCVLLSPFLKGI
                     HWTFNVRLWRQVMAYSLPLTVAGFGGMINETFDRIMLDWRLPHANNYAAYQVGIYSGC
                     YKLSLLITVFVQAFRMGAEPFFFRQSVEESAQRTYARVLKFFVILVCGMFLFVMMYVD
                     ILKYFIADHSMWVGLKVVPILLFANMFLGIYYNLSIWYKLSANTRSGAYITLIGAAIT
                     LVVNYVFIPSFGYMASAWATFLCYGSMMVISYVWGQRVYPVPYAWKKLLAYMVIVALL
                     YFVYLGLTGLWESRIYRLAVGTVLLAGYVLFILRVERREFRRLPVIGKYLGGTRPATA
                     ADAA"
BASE COUNT         1125 a         1258 c         1037 g          956 t
ORIGIN      
        1 tccggtaatc ggtcatcctt gccgtgatat tgcgcttggc aagcgccgca tcatccacct
       61 tgagcttata aaggaatttg atatcgccct cctgccgcac ctcactcacc tgcccgttgt
      121 cccccgcgct ctttgtctcg accaggctgc tctggtagtt ggtgatcggg aagacatacg
      181 ccgaatcatt ggagagcgac accacatagg aagtgtccgg ctgctgcctg ttataagcct
      241 tcaatgtgga atcaagatcg ctgggcatcg ggttgtgcaa caactcatcc ccgatcttgt
      301 acaccgtatc aagcccggca cgctgcgtat ggaagaaacc ggcatagcgg ttggcgatcc
      361 cattctcgtc actcacaaac gtaaagtgat tggaattgta ctgcatcggg taacgcgcat
      421 tcccatactt cagatacgta agctgggaca cctgcttccc ttcattatcg ttccagttat
      481 cgacaagaaa gatattgtaa tgatagttgg aaggcaatac ggtatccttg ctgacggcat
      541 gggccgacgg gcggttcgag gaatagatga tccccgtctt gcccggaaag gcgataaagg
      601 tcgcatccag gtcatcatag atatcgttcg tgatctgccg gtaactctgt ttctcgatat
      661 catagacata gatatccgac tggccattgc gcacggcgct caggatcagc gtattgttgt
      721 tcagcatgta cttgaagtcc tgcacctgct gaaaatccgg cagatcctgg ttgatcagct
      781 tgtacttccg tacaacatca tagacaaaaa gcctggtctt ccccctgtcc caatagatac
      841 aggccagcct cgttcccttg gggtcccagg caagcagggg atagtgagga ttgatctgct
      901 cctgcagcga acgcaccccg ttcttcagca ataccttggt aaaaatataa ttgtcatgca
      961 gcgtgaccgt atacttcccc cggttgtact ccaccaccgc ataggtctgg ctcttgggta
     1021 ccgggttggg gctgaaatgg aagaaatcct tatggtcgtt gatgtcctcg gtcaccgaaa
     1081 ccgtcccctt ggggaaattc cgccgcccgc ggaggtcctt ctcatacttt tcctcctcct
     1141 ccgccataaa gtcctttaac acctccttga acttcttctt gcagatccgc agcgaagcat
     1201 tgttcaggtt gcggtacacc cgcgataaat aaaggaaata cgtgacattt tccttcttgt
     1261 acttgtcggc aagataatac cagaaggcat gacccgcgag attgggccgc tcataggcaa
     1321 actggtagaa attcttgtat cgcccggaca gcatcgacga tttcaactga tcgtccagca
     1381 ccgtattcca gttctccgcc gcatactcga tatacccatc cgtaagccac tgcggcaggt
     1441 cgagcagggc ctggttggcc gcaaattccc cgagatcgtc gccaaaaagg atgttctcca
     1501 cgaggatgcg ggcgatcccc tgccggatct ggatgcgaag attgttgtgg tccccgttgt
     1561 aatagaccac catcttgtta ttcactaatt tggtgatccc ccccgtcgtc tgccagtcca
     1621 ggttaagccc gatattggac tgctccatgt cattgtacgt gttgtaaatg acaatattgg
     1681 cccggcgctg caacccgtat tccacgaatt tctcgatgcc cggaagctct ttttccgcca
     1741 gctgcaggac aaatttgccc agatcctgcc ccccctggtt aaagtaggta ttgaaattgg
     1801 tcgtctggta gtactgccat ttgaacttct tgaattgcag gcggtttttg ccgaattcca
     1861 ccgtattcac ctgggccttg agggccccgg aaaagaagaa gatgcctgaa aaaatccaaa
     1921 gaaaatagcg tgacttcaac ctgaactgat gaaattgcat acctgctttc ctttaaaggt
     1981 tttaaagtta taacaatccg acctaccggt gaaattactt tatatattaa attatgcgct
     2041 atgctaaccg taaagtcctt cgcgttcaat cccgtgcaag agaacacata cgtggtatct
     2101 gatgaaaagg atgcctgctg catcatcgat ccgggctgtt atttcggcaa cgaacgcacc
     2161 gcattaaagg aatatattga gcagcagggg ctaagcccaa tattcctgct taacacccat
     2221 tgccacctcg atcatgtttt tggaaataag ttcgttcacg acacctggga cttacccctc
     2281 catctccacg aaaaagaagt acccgtcctg gccgccgccc ccgaatgggg cctcgattgg
     2341 ggcatgccct tcgaaaacta ccggggcgaa ttgatcttcc tcacccccgg ccgctccgtg
     2401 accctcgggg accatcgcct ggacatcctc ttcctgcccg gacactcccc cggcagcatc
     2461 ggcttctata acgcggcaca gggcttcctc atcggcggcg acgtcctctt ccgggaaggc
     2521 atcggccgca ccgacttccc cggcggcgac cacgccaccc tgctggagag catccatacc
     2581 cagctctggc cactgcccga cgacaccatc gtctaccccg gacacggcga acccacgacg
     2641 attggatggg agcgggcgca taatccattc ctgaaatgac agcgacccac aagcccgctc
     2701 acctcgcgaa gcgaggtcat cattggcccc ggttaggctg cgtcagcagc cgtagccggg
     2761 cgggtaccgc ctaaatactt cccgatcacg ggcagcctcc ggaactccct ccgctccacc
     2821 cggaggataa aaaggacata accggccagc aggaccgtac ccaccgccaa ccggtagatc
     2881 cggctctccc agagacccgt cagcccgaga taaacaaaat acagcaacgc cacgatcacc
     2941 atgtacgcca gtagcttctt ccaggcatag ggaacaggat acacccgctg cccccataca
     3001 taggaaatga ccatcatgct gccataacaa aggaacgtcg cccaggcgga agccatatag
     3061 ccaaaggaag ggatgaagac ataattcacc accagcgtga tcgcagcccc gataagggtg
     3121 atatacgcgc cggaacgggt attggcagaa agtttatacc agatggaaag attgtagtag
     3181 atgccaagga acatattggc aaaaagcagg atcggcacca ccttcaaccc gacccacatc
     3241 gaatggtcgg cgataaaata cttcaggata tcgacataca tcatcacgaa aaggaacatc
     3301 ccgcatacaa gaataacaaa gaacttcaac acccgggcat aggtccgctg cgccgactct
     3361 tccacactct ggcggaaaaa gaaaggctcg gcccccatcc ggaaagcctg cacaaagacg
     3421 gtgatcagca acgatagttt ataacatccg ctgtaaatac ccacctggta ggccgcataa
     3481 ttgttcgcat gcggcagccg ccagtccagc atgatccggt cgaaggtctc attgatcatc
     3541 cccccaaaac ccgccaccgt aaggggtagg gaataggcca tcacctgccg ccacaaccgc
     3601 acgttgaagg tccagtggat acccttcagg aagggcgaca acaacacaca ttgaaagata
     3661 ttctggatga cattggccag taacacatac cccacatagc catacctggg ctgataaata
     3721 agcgcgataa tactgttcgg atgggtcttt aacagccgcg gacacgccga caggaaaaag
     3781 aagacaagcc cgacatacaa aatgatcccc gaaatccgga taagcgcata tttccgcggc
     3841 ctcccctcat gccgcagcct cgcgaacggc agcgccgaca gggcatcgaa ggcgatcatc
     3901 agggcgctaa gggtgatata cccggggtga ccacccacat cgaggaccgt ggctatcgga
     3961 ccacgaaaaa gaatggtcgc cgtggtgatc agcatagtac tggacaccag cgatagcagc
     4021 agcgtcgaat aaagatcacc cgacgtctct tttttattga tataccggaa atacgcgtag
     4081 tcaagaccga acaacacccc gatattgata aaggaaatgg cagcatagac aaggctcatc
     4141 tgcccaaact cgggcgtgcc tctgaacttc gcggtcaggt aaggggtcag cagctgaacg
     4201 atgaatttgg cgacaatgga actcacccca taccagaccg tctcacccgc taatttttta
     4261 atactgctca aatcattgtt ttatcgctgc aaaattatct tagaacgagg atatcagcgt
     4321 ctttatttcg agatcgaaaa taagtgttaa cacttagatt caaaggatct cctcct
//
LOCUS       DATGFG010000094         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_332688, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000094 DATGFG010000000
VERSION     DATGFG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332688"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VKQ52_01790"
     CDS             <1..373
                     /locus_tag="VKQ52_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85935.1"
                     /translation="QNNMAYAKVQNKAGNYITPSIASVTAAANIQIPADTKVSLTNTD
                     AADGYPISSFSWVIIYKEQKYSDRTLDRATRLVKLLSWMIHDGQQYSSALTYAPLSPA
                     AVSAGDAVLKSVTFDGKPILQ"
     gene            403..1389
                     /gene="pstC"
                     /locus_tag="VKQ52_01795"
     CDS             403..1389
                     /gene="pstC"
                     /locus_tag="VKQ52_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HLZ85936.1"
                     /translation="MDTSVENGTITRSELPLRVITSSPGAIARAKARAKRSFRQIRTE
                     NVFKRTLMIMGLLMIVLVLGICLTLAWQSRLSISTLGFKYLWSKVWDPVQDIYGAWPF
                     LLGTLLTSFLALIISIPFSISIAIFLGEYHPKGWLPNFLKNAVELIAAVPSVIYGFWG
                     LAVLVPIVRSFEASIGVAPIGVGIFSSSLILAVMVIPYAASLGRSMITLVPSHLKEAA
                     YGLGATRWEVIRSVVLPHTKSGLFAGILLSLGRALGETMAVTMVIGNTSLIPKSIFAP
                     GNTMASVIANEFTEADKPVYLSALIELGLVLFLVTVVINLIGKKIIDRFTNE"
     gene            1394..2266
                     /gene="pstA"
                     /locus_tag="VKQ52_01800"
     CDS             1394..2266
                     /gene="pstA"
                     /locus_tag="VKQ52_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HLZ85937.1"
                     /translation="MDGKNIKLRLGKSYGFQWLIYILAFACLLPLLAILFYIVRAGIT
                     KINWHFLVNIPKPVGESGGGIANALVGSTLIILVASVIAIPIGIMAGVYLSENRRSRL
                     AYWCRLSTDILQGVPSIVVGIVAYFWLVRPWDIGFIHHKGGFSALSGSVALAIMMLPI
                     IVRSTEETLKLLPDSLKEAALALGMPFHRVIFKVIVPVGISGILSGVMLSIARITGET
                     APLLFTAFGNPYLSVNIGKPMQSLPLLIFNYATSPYTDWLNLAWGASLILLIWVLFLN
                     IFTKVITRKWKVQL"
     gene            2248..>2854
                     /locus_tag="VKQ52_01805"
     CDS             2248..>2854
                     /locus_tag="VKQ52_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HLZ85938.1"
                     /translation="MESTIIKSRNFNASFGSNHVVKNVNIDIPRNHIIAVMGPSGCGK
                     TTYLRCINRMHELIPGATSNGEMRLNEEDVYQMHPIFVRLKIGMVFQRPNPFPNLSIY
                     DNVIAGYKLNGIKLPKAERDEIVERSLTKVAMWKEVKDSLQKKGSFLSGGQQQRLCIA
                     RTVAMEPEVILFDEPTSALDPISTSTVEELFHELKANYTLII"
BASE COUNT          579 a          837 c          756 g          682 t
ORIGIN      
        1 tcaaaacaac atggcgtatg ccaaggttca gaacaaggcc ggcaactata tcacccccag
       61 catcgccagc gtaactgctg ccgcgaatat ccagatcccc gccgatacaa aggtatccct
      121 gaccaacacg gatgctgccg atggatatcc catctccagc ttctcctggg tcattatcta
      181 caaggagcag aaatatagcg accgtacgct tgaccgcgct acccgcctcg tgaagctcct
      241 ttcctggatg atccatgacg gtcagcagta cagcagcgca ctgacctatg ccccgctctc
      301 tccggctgcc gtcagcgccg gggacgcggt tttgaaatcg gtcacttttg atggaaagcc
      361 tatcttgcag taatatgtta atgtgtaact gaatagctat ttatggatac atctgtagag
      421 aatgggacga ttacccgcag cgagctaccg ctgcgggtaa ttacttcttc ccccggcgcg
      481 atcgcaaggg caaaagcgcg ggcaaaacgc tctttccggc aaatacgcac ggaaaatgtt
      541 tttaagcgga cgttgatgat catgggtctg ctcatgatcg tgctggtgct gggtatctgt
      601 ctgaccctgg cctggcagtc ccgtctgtcg atctcgacgc tgggcttcaa atacctttgg
      661 agcaaggtgt gggaccccgt ccaggatatt tacggcgcct ggcctttttt gctgggaacc
      721 ctgctcactt ccttcctggc gctgatcatc tccatcccgt tctccatttc gatcgccatt
      781 ttcctggggg aataccatcc caagggctgg ctgcccaatt tcctgaagaa tgcggtagag
      841 ctcatcgccg ccgtgccttc ggtaatttat ggtttctggg ggctggccgt gctggtgccc
      901 attgtccgga gcttcgaggc cagcatcggt gtggcgccca tcggcgtcgg gatcttcagc
      961 tcttcgctga tcctggccgt catggtcatc ccctatgccg cctcgctggg ccgcagcatg
     1021 atcaccctcg tcccttcgca tttgaaggag gcggcttatg ggcttggcgc cacccgctgg
     1081 gaggtgatcc ggagtgtagt gctgccgcat accaagtcgg gtttgtttgc cggcatcctt
     1141 ctttcactcg gtcgtgcatt gggagaaacg atggccgtca cgatggtcat cgggaatacc
     1201 agcctgatcc ccaagagcat ctttgcgccg gggaacacca tggcgagcgt tattgccaat
     1261 gaattcacgg aggcggacaa gccggtatac ctgtcggcgc tgatcgaact cgggctcgtg
     1321 ctcttcctgg tcaccgtcgt gatcaatctc atcggcaaga aaatcatcga tcgcttcacc
     1381 aacgaataac gttatggacg gaaaaaatat aaaactgcgg cttggcaaga gctatggctt
     1441 ccagtggttg atctatatcc ttgcctttgc ctgtcttctc ccgctgctgg ccatcctttt
     1501 ctatatcgtc agggcgggca tcaccaagat caactggcat tttcttgtca atattcccaa
     1561 gcccgtcggc gagtccggcg gcggcattgc caacgccctt gtcggcagta ccctcatcat
     1621 ccttgtcgcc agcgtcatcg cgatccccat cgggatcatg gccggcgttt atctcagcga
     1681 gaaccgcagg agccggctgg cttactggtg ccggctgtcg acggatatcc tgcagggtgt
     1741 accctccatc gtcgtcggta tcgtcgccta tttctggctg gtgcggccct gggatattgg
     1801 attcatccat cacaaggggg gcttctctgc cctttccggc agcgtggccc tggcgatcat
     1861 gatgttgccc atcatcgtgc ggtcgaccga ggagacgctg aaactgttgc cggattcgct
     1921 gaaagaggcc gcgctggccc ttggtatgcc ctttcaccgg gtgatcttca aggtcatcgt
     1981 gccggtaggc atcagcggca tcctctccgg cgtcatgctt tcgatagccc gcatcaccgg
     2041 cgagaccgca cccctgttgt ttacggcatt cgggaaccct tatctgtccg tcaatatcgg
     2101 caagcccatg caaagcctgc ccctgcttat cttcaactac gccaccagcc cttacacgga
     2161 ttggttgaac ctggcctggg gcgcatcgct gatcctcttg atctgggtct tattcctaaa
     2221 catctttacc aaggttataa ctcgaaaatg gaaagtacaa ttataaagag caggaacttc
     2281 aacgcttcgt ttggtagcaa tcacgtcgtc aagaatgtaa atatcgatat cccccgcaat
     2341 catatcatcg ccgtgatggg cccttccggc tgtggtaaga ccacctatct gcgctgtatc
     2401 aaccggatgc acgagcttat ccccggcgcg acctccaacg gggaaatgag gctcaatgag
     2461 gaggacgttt accagatgca tcccatcttc gtccggctca agatcggcat ggtcttccag
     2521 cgcccgaacc cctttcccaa cctgagcatc tacgataatg tcattgccgg ttacaagctg
     2581 aacggcatca aattgccgaa ggccgaacgg gatgagatcg tggagcggtc gctcaccaag
     2641 gtggccatgt ggaaggaagt aaaggattcg ctgcaaaaga agggatcgtt cctgtccggt
     2701 ggccagcagc aacggctttg tatcgcgcgc accgtggcca tggagccgga ggtgatcctt
     2761 ttcgacgagc ctacttcggc gctggacccc atctccacct ctacggtgga ggagctgttc
     2821 catgagctga aggccaacta taccctcatc atcg
//
LOCUS       DATGFG010000095         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_780968, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000095 DATGFG010000000
VERSION     DATGFG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780968"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..667
                     /gene="leuD"
                     /locus_tag="VKQ52_01810"
     CDS             62..667
                     /gene="leuD"
                     /locus_tag="VKQ52_01810"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HLZ85939.1"
                     /translation="MSASIDKITSTTVPVNIDNVDTDQIIPARFLKATSRDGFGKNLF
                     RDWRYNPDGSPKKDFILNNPTYSGKILVAGKNFGCGSSREHAAWAIKDAGFDVVISSF
                     FADIFKNNALNNFLLPVTVSEDFLQKIFAAIEKDPGTTLSIDLAAQTITLAGRTEPFE
                     INDYKKTCLLNGYDDIDFLLSIRPEIEAFEQQHAATSNAMQ"
     gene            698..1813
                     /gene="leuB"
                     /locus_tag="VKQ52_01815"
     CDS             698..1813
                     /gene="leuB"
                     /locus_tag="VKQ52_01815"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HLZ85940.1"
                     /translation="MQKNIVVIEGDGIGPEVTRQAIKVLDAVAEQGGHEFTYTYCLMG
                     ACAIDKTGNPLPDETIEAALNSDAILFGAIGHPKYDNDPTAKVRPEQGLLKLRKSLQL
                     FANIRPVTTYPALQHLSPLKAKILEGVDFIIFRELTGGIYFGKKETNAEGTQASDDCV
                     YTREEIERITHLAFKKAQQRRKKLTLVDKANVLETSRLWRRVVQEIAGQYPDVKVDFL
                     FVDNAAMQIILNPKQFDVILTENMFGDIISDEASVISGSLGLLPSASIGKGSALFEPI
                     HGSYPQAAGKDIANPLGSILSAAMLLDHFGLHTEANRIREAANWTLQNGFVTKDIDPI
                     NFYFTSTIGELISDYVSGKTPGDIHRDNIELRKSTII"
     gene            2055..>2503
                     /locus_tag="VKQ52_01820"
     CDS             2055..>2503
                     /locus_tag="VKQ52_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HLZ85941.1"
                     /translation="MASYYNEHTVLWLDGTILKASETKTDLYGQSLHYGYSVFEGIRS
                     YRTAAGKTKIFKAVEHYDRLQRSAAALNMPYRYTTAELINATYDVLERNNLQDAYIRP
                     VVYGPANMSFNPNTESFTVIQTWEMAPFLGEKLLRVMTSSFQRPNPRG"
BASE COUNT          667 a          839 c          554 g          443 t
ORIGIN      
        1 gccgtcgccg ccatcacagg cgaagtagcc gatatccgtg aatacatcta atcccaaaac
       61 catgtctgca tccatcgata aaataacatc caccaccgta ccggtcaaca tcgacaacgt
      121 cgacaccgac cagatcatcc ccgcccgctt cctcaaggcc accagccgcg acgggttcgg
      181 caagaacctc ttccgggact ggcgctacaa cccggacgga agccccaaaa aggatttcat
      241 cctcaacaac ccgacctata gcggaaagat cctggtagcc ggcaagaact tcggctgcgg
      301 ctccagccgg gaacacgccg cctgggccat caaggacgca ggcttcgacg tcgtcatcag
      361 cagcttcttc gccgacatct tcaaaaacaa cgccctcaat aatttcctcc tgccggtcac
      421 cgtcagcgaa gactttctcc aaaagatctt cgccgccatc gaaaaagacc ccgggacgac
      481 cctttccatc gacctcgcag cccagaccat caccctcgcc gggagaacgg agcccttcga
      541 gatcaacgac tataaaaaga cctgcctgct caatggatat gacgacatcg acttcctgct
      601 gagcatccgg cccgagatcg aagcattcga acaacaacac gcggcaacaa gcaatgccat
      661 gcaataaaat tataccctta cccaatcaac taaatctatg caaaagaaca tcgtagtcat
      721 cgaaggtgac ggcatcgggc ccgaagtgac ccgccaggcg ataaaggtcc tggacgccgt
      781 agccgaacaa ggcggtcacg agtttaccta cacgtactgc ctgatggggg cctgcgccat
      841 cgacaagacc ggcaaccccc ttcccgacga gaccatcgaa gccgcgctca acagcgacgc
      901 catcctcttc ggcgcgatcg gccaccccaa atacgataac gaccccacgg caaaggtccg
      961 ccccgaacag ggcctgctca aactgcggaa atccctgcag ctgtttgcca acatccgccc
     1021 cgtgaccacc tacccggccc tgcagcatct ttcccccctg aaggcaaaga tcctcgaagg
     1081 cgtggacttc atcatcttcc gcgaactcac cggcggtatc tacttcggga aaaaagagac
     1141 caacgccgaa ggcacacagg catccgacga ctgcgtctac acccgcgaag agatcgaacg
     1201 catcacacat ctcgccttca aaaaggcgca acaacgccgc aagaagctga ccctcgtcga
     1261 caaagccaat gtcctggaaa cttcccgcct ctggcgcagg gtcgtccagg agatcgccgg
     1321 ccagtacccc gacgtaaagg tcgatttcct cttcgtcgac aacgcagcca tgcagatcat
     1381 cctcaatcca aaacaattcg acgtcatcct gacggaaaat atgttcggcg atatcatcag
     1441 cgacgaagcc agcgtcatca gcggaagcct gggcctcctg ccctccgcct ccatcggcaa
     1501 agggtcggcc ctcttcgagc ccatccacgg ctcctacccg caggccgccg gcaaagacat
     1561 cgccaacccc ctgggcagca tcctctccgc agccatgctg ctggaccatt tcggactgca
     1621 caccgaagcg aaccggatac gggaagcagc caactggacc ctgcagaacg gcttcgtgac
     1681 aaaagatatc gatcccatca atttctattt cacgtccacc atcggcgagc tcatcagcga
     1741 ctacgtcagc ggtaagacgc cgggagacat ccatcgggat aatatcgagt tgagaaagtc
     1801 gacgatcatt taagaaagtc gcaaaaaagt tctttgtatt ttcaattcgt cggagtattt
     1861 tcgcaaaaga aacatcctcc gtgaacaaac gagtagtaaa tatcaccaag cagattctag
     1921 ccgcaattat tattgtggta gtggtgatta ttattccggc tctgggagga gggtggtaac
     1981 ttaaataaaa taaagtaaaa gataccagcg gcccgcctcc ccaaagagac gggccgcttt
     2041 tttaattgca tcttatggca tcatattata acgaacacac agtcctctgg ctcgacggca
     2101 cgatcctcaa agcctcggaa acaaagaccg acctctacgg ccagtccctt cactacggct
     2161 actccgtatt cgaaggcatc cgctcctatc gcaccgccgc cggaaagaca aagatcttca
     2221 aggccgtcga acactacgac cgcctccagc gctccgccgc ggccctcaac atgccctacc
     2281 gctacaccac cgccgaactc ataaacgcca cctatgacgt cctcgaacgc aacaacctcc
     2341 aggacgctta catccgcccc gtcgtatacg gcccggccaa tatgagcttc aaccccaata
     2401 cggagtcttt caccgtcatc cagacctggg aaatggcgcc cttcctcggc gaaaaactcc
     2461 tcagggtaat gacctcctcc ttccaacggc ccaatcccag ggg
//
LOCUS       DATGFG010000096         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_780721, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000096 DATGFG010000000
VERSION     DATGFG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780721"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2203)
                     /locus_tag="VKQ52_01825"
     CDS             complement(<1..>2203)
                     /locus_tag="VKQ52_01825"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HLZ85942.1"
                     /translation="CIKVKFTDRQTGFILNNNGDLFKTTDQGGHWQRTDHFSRVLCMD
                     IAYSTGVIAGDSGVAYMSHDHGVSWSAVKTGTTDALSMISIVSADTLFLGSTTGNLYE
                     TTDGGNSWSALTCQHQINCTWFVDSKTGFAGGPGSLILKTVDGGVTWQQKEQSNSFPS
                     GILAIQFLNAQTGYALREYYDVQVTHDGGNTWATYNGKYQMTAIDMVDATVGYIAGEN
                     GAICQTTDGGMTWNPVIPSNGFKDGYQLYSLHFISADTGFIVGLAGRILKTVDGGTTW
                     NRYSPTYLPITAVSFPTPAVGYAADWNYTYKTTDSGRSWVPLSLTTGTSYASQSRFEQ
                     AHFINADTGFFVSNGYFQLHTTTDGGQTWTASNPAVYSWDDAPRLCFVDSRNGFLSVT
                     NYNTGLILRTRDGGLSWTQAWATGSGQPYYLGRVSFADTLTGFGIESQNLYKTADGGT
                     SWQPVYSPAPGGQYTTLTDVCFTDKQNGFIGNSGDGLAVTHDGGSSWATVSTPYTSGG
                     GINAIRFFNGKIGYFTDGNQFGPAGYGNIYRTLDGGVTWRLDMNFGGTGIGFTPDSNV
                     VVNDFAGGLLKTGIRAGRADSLSTGLGDDCNKDLSAIVSVPIGEVDSIAFEIVAADGS
                     IQRVAASPASVSNGRATCTAATPPLKPDVAYAVRLRFVENGVAWYGDPARFVASNLPR
                     PSIYDSSGVLRSSADSGNQWFLNGAAIANAVRPEYTPKATGRYSVQVTRGGC"
BASE COUNT          478 a          721 c          575 g          429 t
ORIGIN      
        1 gacatccgcc ccgggtgacc tgcacgctat accggcccgt cgccttcggc gtatactccg
       61 gacgtaccgc attggcgatg gccgcgccat tgaggaacca ttgattgccg ctgtccgcgg
      121 aggatctcag cacacccgaa ctgtcataga tggaaggccg ggggagatta ctggctacaa
      181 accgcgccgg atcgccgtac catgctaccc cgttctccac aaaccgcagc cgcaccgcat
      241 acgcgacatc cggcttcagc ggcggtgtag ccgccgtaca ggtagcccgg ccattgctca
      301 cgctcgccgg cgacgccgcc acccgctgga tactgccgtc ggccgccaca atctcgaagg
      361 cgatgctgtc cacttcgccg atcggcacac tgacaatggc ggagagatcc ttgttgcagt
      421 catcccccag gcccgtgctc agcgaatccg cgcgtcctgc gcgaatgccg gtcttcagca
      481 acccgccggc aaaatcattc accaccacat tgctgtcagg cgtaaagccg atacccgtgc
      541 cgccaaaatt catatccagc cgccacgtca caccaccatc aagcgtccgg tagatattcc
      601 catagcccgc gggaccaaat tggttcccat cggtaaaata accgatcttt ccgttgaaga
      661 agcggatcgc attgatcccg ccccccgagg tataaggcgt agacacagtt gcccaactgc
      721 tgccgccgtc atgcgtaacg gccagcccat ccccgctgtt cccgataaac ccattctgct
      781 tatcggtaaa acagacatcg gtaagtgtgg tatactgccc cccgggagcc ggcgaataca
      841 ccggctgcca gctcgtaccc ccgtcagccg tcttgtaaag gttctgcgat tcaatgccaa
      901 agccagtcag cgtatccgcg aaagacaccc ggccaagata gtacggctgc ccgctacccg
      961 tcgcccaggc ttgggtccag ctaagcccgc cgtccctcgt ccggaggata agcccggtat
     1021 tataattggt gacggagaga aatccgttcc tgctatccac aaaacaaagg cgcggcgcat
     1081 catcccagga ataaacagcc ggattactgg ccgtccaggt ctgcccgcca tccgtagtgg
     1141 tatgcagctg gaaatagcca ttggatacaa agaaccccgt gtcggcattg ataaaatgcg
     1201 cctgctcgaa acggctctgg ctggcatagc tcgtacccgt cgtcaggctc agcggaaccc
     1261 agctgcgacc gctgtccgtc gtcttatacg tatagttcca gtccgccgca tagccgacgg
     1321 caggcgtcgg gaaagacacc gcggtgatcg gcagataggt cggggaatac cggttccagg
     1381 tcgtgccgcc atccaccgtc ttcaggatcc ggcctgccag cccgacgata aacccggtgt
     1441 ccgccgatat aaaatgcagc gaatacaact ggtagccatc tttgaaccca ttggagggga
     1501 tcaccgggtt ccaggtcatc cccccatctg tcgtctggca gatagccccg ttctcgcccg
     1561 caatataccc caccgtcgca tcgaccatat cgatcgccgt catctggtat ttcccattgt
     1621 aggtagccca ggtattgccg ccgtcatggg tcacctgcac atcgtaatac tcgcgcaggg
     1681 cataaccggt ctgcgcattc aggaactgta tcgccaggat gcccgaggga aaactattcg
     1741 actgctcctt ctgctgccag gtcactccgc catccaccgt cttcaggatc agcgatcccg
     1801 gtcccccggc aaaaccggtc ttcgaatcga cgaaccaggt acaattgatc tggtgctgac
     1861 aggtcagggc cgaccagctg ttgccgccat ccgtcgtctc atagagattg ccggtagtgc
     1921 tgccaaggaa cagggtgtcg gcactgacga tgctgatcat cgaaagggca tcggtggtcc
     1981 cggtcttcac cgccgaccac gacaccccgt ggtcatggct catatacgcc accccgctat
     2041 ccccggcaat gaccccggtg gaataggcga tatccataca aagcacgcgg gaaaaatgat
     2101 ccgttcgctg ccaatgaccg ccctggtcgg tggtcttgaa cagatcgcca ttattgttga
     2161 ggataaaacc ggtctggcgg tcggtaaatt ttactttgat aca
//
LOCUS       DATGFG010000097         4346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_439603, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000097 DATGFG010000000
VERSION     DATGFG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439603"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VKQ52_01830"
     CDS             <1..212
                     /locus_tag="VKQ52_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85943.1"
                     /translation="EEVVRKTLKTINDKLIEFKTQFAGRDMQDYIAMVVLWYATQPAE
                     EGGMGVNPAVLDALARMEQQLDKVL"
     gene            373..1938
                     /gene="rny"
                     /locus_tag="VKQ52_01835"
     CDS             373..1938
                     /gene="rny"
                     /locus_tag="VKQ52_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HLZ85944.1"
                     /translation="MTENVIVVIVGLVALVVGVFVGKVLFKKNVQGKVDEAERQAKKL
                     VADAQTAAENLKKEKLLEAKEKFVQLKAEHDKEVLEKNRRANDAESRIKQKEQGINQK
                     LENLDKQAKDNEAIKENLNRQIEVVSMKRTELEKHQEEHIRRLEKIAGLTAEEAKSQL
                     VESLRQEAQSRALALQQEIIDDAKQKANKEARKIIIQSIQRTAAEQAIENSITVFNLE
                     SDEIKGQIIGREGRNIRAIEAATGVDLIVDDTPEAIVLSSFDPLRREIARLSLQRLVS
                     DGRIHPARIEEIVEKTRKQIEEQVMEIGERTVIELGIHGLHKELVRIVGKMRFRSSYG
                     QNLLMHSREVANLCGIMAAELGMNPKLAKRAGLLHDIGKVPDEETELSHALLGMKLAE
                     KYGENPAVVNAIGAHHDEVEMQYVIAPIIQACDAISGARPGARREIMQQYLQRIKDLE
                     NLALAYPGVEKAYAIQAGRELRVIVEADKVTDVDSDKLSFEIAQKIQTEMTYPGQIKV
                     TVIREKRAVNVAR"
     gene            1997..2359
                     /gene="rplS"
                     /locus_tag="VKQ52_01840"
     CDS             1997..2359
                     /gene="rplS"
                     /locus_tag="VKQ52_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLZ85945.1"
                     /translation="MNAAVAFVHEQLTGKKEFPKFKAGDNITVNYKIIEGNKERIQSF
                     KGDVIKRQGTGHTACFTVRKISDGVGVERTFPLFSPNIESIDLNKVGRVRRAKLYFQR
                     SRSGKSARIKEKRMATEA"
     gene            2454..2684
                     /locus_tag="VKQ52_01845"
     CDS             2454..2684
                     /locus_tag="VKQ52_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HLZ85946.1"
                     /translation="MTTFLISMPGGSEWVLIILAVLVLFGGRKIPEFMRGLGKGIREF
                     NDAKNNVKKELEDGMNEKDTKPASPASTPSQQ"
     gene            2717..4120
                     /gene="gatA"
                     /locus_tag="VKQ52_01850"
     CDS             2717..4120
                     /gene="gatA"
                     /locus_tag="VKQ52_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HLZ85947.1"
                     /translation="MQDYHKALRDRTASCEEAVLHYLTAIDRQQHLNAYIRVYGEEAL
                     AKARLLDQRRREGRPMGKLHGVVIGLKDVLAYKDHPLTASSHILQDFVSIYNATVVEK
                     MLAEEAILIGHLNCDEFAMGSSNENSAYGKVLNARGSDRVPGGSSGGSAVAVQAGLCM
                     ASLGSDTGGSVRQPADFCGILGLKPTYGRVSRYGLIAYASSFDQVGIFALSVGDIGLL
                     LEVIAGEDDYDSTVKQEAAPVWEETPARKYRIAYFREALDHPGLDPEIRERILERTAE
                     WREAGHTVEAIDFHLLDYIVPAYYVLTTAEASSNLARFDGVRYGYRTKDPGLELTEFY
                     KRTRSEGFGREVKRRILLGTFVLSAGYYDAYFTKAQQVRKLVVDRTNLIFKDFDIIIL
                     PTAPTTAWRIGEKMDDPISMYLADIYTVYANLTGIPGISLPLFGHTNGMPFGIQAMTS
                     RFNELSLLQFSHQMMQQ"
BASE COUNT         1125 a         1180 c         1170 g          871 t
ORIGIN      
        1 acgaagaggt ggtcagaaag accctcaaga cgatcaacga taagttgatc gagttcaaaa
       61 cccagttcgc gggccgggat atgcaggact atatcgccat ggtggtcctg tggtacgcca
      121 cccagccggc ggaagaagga ggcatgggcg tcaatcccgc ggtactggat gccctcgctc
      181 gcatggaaca gcagttggac aaggttttat agcggctctt tgcgtggaac atcccatttt
      241 tggagtcgat ttactatctt cgccaactaa cgaaaaagac ggaataggaa tccgtcggaa
      301 tttttattat tcatacaatc atgacactca atatgaatca tatgattgtg aacgctttaa
      361 atacaaaatt caatgacaga gaacgttatt gtcgtcatag taggactcgt cgccctggtt
      421 gtaggggtct ttgttggtaa ggttctgttc aaaaagaatg tccagggaaa agtcgacgaa
      481 gccgaacggc aggccaaaaa gctggtagcg gacgcccaga cagccgccga gaacctgaaa
      541 aaggaaaaat tactggaagc aaaggaaaaa tttgtacaac taaaggcgga gcacgacaag
      601 gaagtgctgg aaaagaaccg gcgagccaat gacgcggagt cccggatcaa gcagaaagaa
      661 cagggtatca atcagaaatt agagaatctc gacaagcagg ccaaggataa tgaggccatc
      721 aaggaaaacc tcaaccgcca gatcgaagtc gtcagcatga aacgcaccga actggaaaag
      781 caccaggaag aacatatccg ccgcctcgaa aagattgccg ggctcaccgc cgaggaggct
      841 aagtcgcagc tggtggaaag cctgcggcag gaagcccaga gccgggccct tgcgctgcag
      901 caggagatca tcgacgacgc caagcagaaa gcgaataaag aagcccggaa gatcatcatc
      961 cagagcatcc agcgtaccgc cgccgaacag gccatcgaga attcgatcac tgtcttcaac
     1021 ctggaaagtg acgagatcaa gggccagatc atcggccgtg aaggtcgtaa tatccgggcc
     1081 atcgaagccg cgacgggtgt ggacctcatc gtggatgaca cccccgaggc catcgttctt
     1141 tcctcttttg accccctgcg cagggagatc gcccgccttt cactccagcg actggtatcc
     1201 gacggccgta tccacccggc gaggatcgag gagatcgtcg aaaagacccg gaaacagatc
     1261 gaggaacagg tgatggagat cggtgaacgt acggttatcg aactgggtat ccacggttta
     1321 cataaagagc tggtacgcat cgtcggaaag atgcgcttcc gctcctccta tggtcagaac
     1381 ctgctgatgc acagccggga agtggccaac ctctgcggga tcatggccgc ggaactgggg
     1441 atgaacccca agctggccaa gcgggccggc ttattgcacg atatcggaaa ggttcccgac
     1501 gaggagaccg aattgagcca cgccctgctg gggatgaagc tggcggagaa gtatggagag
     1561 aatcccgccg tcgtcaacgc gatcggcgcc caccacgacg aagtggagat gcagtatgtc
     1621 atcgccccga tcatccaggc ctgtgacgcc atcagcggcg cccgtcccgg agcccggcgg
     1681 gagatcatgc agcaatacct gcaacggata aaagacctcg aaaacctggc cctggcctat
     1741 cccggtgtcg aaaaagccta tgccatccag gccggccgcg agctccgggt catcgtggaa
     1801 gccgataagg tgaccgatgt cgacagcgac aagctgagct tcgagatcgc tcagaagatc
     1861 cagacggaaa tgacctaccc cggccagatc aaggtgacgg tcatccggga aaaacgcgcc
     1921 gtcaacgtag cacggtaaaa aactttgttt tttacctaaa agcttattac ctttgctgtc
     1981 cttaaaaaaa tgagttatga acgctgctgt agcttttgta cacgagcaac tgacgggaaa
     2041 gaaagagttt ccgaagttca aagccggtga taacattacc gtcaactata agatcatcga
     2101 aggaaacaag gagcgtatcc aaagcttcaa aggcgacgtg atcaagcgcc agggtacagg
     2161 ccacacagcc tgtttcaccg tccgtaagat ctccgacggc gtcggcgtag agcgcacctt
     2221 ccccctcttc tcccccaata tcgagtcgat cgacctgaat aaggttggcc gcgttcgccg
     2281 tgcaaaactg tacttccagc gcagccgcag cggtaagagc gcccgtatca aggaaaaaag
     2341 aatggctacc gaagcgtagt gtaaacagga tattaacaaa aagggccgca aatcacggcc
     2401 cttttttagt tttatatagt acctttacag ctcatctttt aaagactttg attatgacta
     2461 cttttttgat ctcgatgccc ggtggtagtg agtgggtgct gatcatcctg gccgttctcg
     2521 tattgttcgg cggacggaag atccccgaat tcatgcgcgg cctgggtaag ggtatccgtg
     2581 agtttaacga tgccaagaac aatgtgaaga aggaattgga agacggtatg aatgaaaaag
     2641 acaccaaacc cgccagcccc gccagcactc cttcccagca ataaaacctg gacgccggct
     2701 gtttacgttt cactccatac aagactatca taaagcctta agggacagga ctgcttcttg
     2761 cgaagaggcg gtccttcact atttaacggc tatcgaccgg cagcagcatc tcaacgccta
     2821 tatccgcgtg tacggcgagg aagccctcgc aaaggcacgg ctgctggacc agcgacgcag
     2881 ggaaggccgg cccatgggaa aactccatgg cgtggtcatc ggcttaaagg atgtcctggc
     2941 ctataaggac catccgctta ccgcctcctc tcatatcctg caggattttg tctccatcta
     3001 caacgctacg gtcgtcgaaa agatgctcgc cgaagaggcg atcctcatcg gccacctcaa
     3061 ctgtgacgaa tttgccatgg ggtcgagcaa tgagaactcc gcctatggca aggtcctcaa
     3121 tgcccggggc agcgaccggg tgcccggcgg ctcctccggc ggcagcgcgg ttgccgtcca
     3181 ggccgggctc tgcatggcca gcctgggaag cgataccggg gggtcggtcc gccagcctgc
     3241 cgatttctgc ggcatcctgg ggctaaagcc cacctatggc cgcgtctccc gctacgggct
     3301 aatcgcctat gcctcctctt tcgaccaggt cggcatcttc gcgctttctg tcggggatat
     3361 cggccttctc ctggaggtca tcgccggcga agacgactac gacagcacgg taaagcagga
     3421 ggctgcaccc gtatgggagg aaacccccgc ccggaaatac cggatcgcgt atttcaggga
     3481 agcactggac cacccgggcc tcgacccgga gatccgggag cgcatcctgg aacgcaccgc
     3541 cgaatggcgg gaggcggggc acaccgtaga agccatcgac ttccacctgc tcgactatat
     3601 cgttccggcc tattatgtgc tgacgacggc ggaagcctcc tccaatctcg cccggttcga
     3661 cggggtccga tatggctacc ggacaaaaga tcccgggctt gaattaacag agttttacaa
     3721 acgaacccgg agcgaaggct tcggacggga ggtcaagcga cgtatcttat tgggtacctt
     3781 cgtattgagt gcagggtact acgatgccta cttcaccaag gcccagcagg tgcgtaaatt
     3841 ggtggtcgat cgaaccaacc tgatattcaa agacttcgat attattatcc ttccaacggc
     3901 ccccaccacg gcctggcgca tcggcgagaa gatggacgac cccatttcga tgtatctcgc
     3961 tgatatatat acagtttacg ccaatctcac cgggattccc ggcatctccc taccactttt
     4021 cgggcataca aatggcatgc cttttggtat ccaggctatg acgagtcgat ttaatgagct
     4081 atctttgctt cagttctcgc atcagatgat gcaacaataa agtcctacga aaatcctact
     4141 ctatgtgact gagagatgtg aagctgattg acttttctac tctcccgtat tccgtttcct
     4201 atgaaagatt gtagacgccg ataacggcgg cttaccatct ttaaacgttg gataatgtac
     4261 aacaaaaggc ttttattgtt aggcatcacc atcattggct caggatggat gcgccctacc
     4321 cccgctgaca tggcgaccct gccaca
//
LOCUS       DATGFG010000098         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_327096, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000098 DATGFG010000000
VERSION     DATGFG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327096"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VKQ52_01855"
     CDS             <1..284
                     /locus_tag="VKQ52_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLZ85948.1"
                     /translation="PQQLLGMVRDIQLVDLDRKDECCGFGGTFCVAEEAVSVKMGKDR
                     VADHVHHGAEFITGSDMSCLMHLEGILRRQKSPVQVKHIAEILNSKITG"
     gene            281..1660
                     /locus_tag="VKQ52_01860"
     CDS             281..1660
                     /locus_tag="VKQ52_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HLZ85949.1"
                     /translation="MSSHHHKDHAALAAAFNKDESRVDWHDQTLWWIRQKRDKAAASV
                     PDWEVLRDNASQIKDHVLSNLSEYLRQFEEEATRNGIVVHWAADATEHNDIVLSILRN
                     HKVDKMVKSKSMLTEECHLNEFLHDNGVEVVDTDLGERVVQLAEEPPSHIVLPCIHWK
                     KEEIGELFHKHLGTPAGNADPQFLTHAARLHLREKFLTRRVALTGVNFAVAETGEFVV
                     CTNEGNADMGAHLADVHIACMGIEKLIPQRRHLGVFLRLLARSATGQPTTTYSSHFRK
                     PRQGQEMHIVLVDNGRSLHLGRKDFRNSLKCIRCAACFNTCPVYRRSGGHSYHTAVAG
                     PIGSILSPNLDRKKYSDLPFASTLCGSCSNVCPVKIDIHEQLYKWRQVIVQEGNAPAA
                     KTAAMKAMTWVLSNPGVYHTAGRTGRWFMLNVPFTVKNKLNAWYLQRELPEPPKQSFG
                     EWYKKNKRS"
     gene            1657..>2063
                     /locus_tag="VKQ52_01865"
     CDS             1657..>2063
                     /locus_tag="VKQ52_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85950.1"
                     /translation="MSSRDKILDTVKANQPEPRELPPHHTGPAALSDLTPQFIKVLES
                     IGGAAFLVSGYDRVAMILREQFPDANRIITSCPQLTAFAAASSAAAALSAATPADPHS
                     FENVDLAILPAHFGVAENGACWLTEAQMLDRVLP"
BASE COUNT          482 a          676 c          538 g          367 t
ORIGIN      
        1 aaccgcaaca gctcctcggc atggtcagag acatccaact cgtcgacctc gaccggaagg
       61 acgaatgctg cggctttggc gggacgttct gcgtggccga agaagcggta tcggtaaaga
      121 tgggaaagga tagggtagcc gaccacgtcc atcacggggc cgagttcatc accggctcgg
      181 acatgagctg tctgatgcat ctcgaaggca tcctccgccg gcaaaaaagc cctgtacagg
      241 taaaacacat cgcagagata ttaaacagca agatcacggg atgagcagtc accatcataa
      301 agaccacgcc gctctggccg cggcattcaa taaagacgaa tcccgggtcg actggcacga
      361 ccagaccctt tggtggatcc gccagaaacg ggacaaggcc gccgcttcgg tgcccgactg
      421 ggaagtgttg agagacaacg catcgcagat caaggaccac gtcctgtcca acctcagtga
      481 gtatctccgg cagttcgaag aagaggccac ccgcaacggc atcgtcgtac actgggcggc
      541 cgatgcgacg gagcacaatg atatcgtcct ttccattctg cggaatcaca aggtcgataa
      601 gatggtcaaa agcaaatcga tgctcaccga ggaatgccac ctgaacgagt tcctccatga
      661 caatggcgtc gaggtagtcg ataccgatct gggggagcgc gtcgtccagc tggccgaaga
      721 gccgcccagc cacatcgttc tgccctgtat ccactggaag aaggaagaga ttggcgaact
      781 gtttcacaaa cacctcggca ccccggcggg caacgccgat ccgcaattcc tgacccatgc
      841 ggcccgcctc cacctgcgcg aaaaattcct cacccgccgc gtagccctca ccggggtaaa
      901 tttcgccgtc gcggagacgg gggaattcgt cgtctgtacc aatgaaggca atgccgatat
      961 gggcgctcat ctcgcggatg tccatatcgc ctgtatgggc atcgagaaac tgatccccca
     1021 gcgacgccat ctcggcgtct tcctgcgcct gcttgcccgc agcgccacgg gacagccgac
     1081 aaccacttac tccagccatt ttcgcaaacc gcggcagggc caggaaatgc atatcgttct
     1141 cgtcgataac ggccgcagcc tgcacctggg ccggaaggac ttccgcaatt cgttaaaatg
     1201 tatccgctgt gccgcctgct tcaacacctg cccggtgtac cggcgaagcg gagggcatag
     1261 ctatcacacc gctgttgcgg gtcccatcgg gtcgatcctt tcacccaacc tggataggaa
     1321 aaaatattcc gatctgccct ttgccagcac cctctgcgga tcatgctcca acgtctgccc
     1381 ggtgaagatc gatatccacg agcagctcta taaatggcgg caggtcatcg tccaggaagg
     1441 taatgcgccg gccgcaaaga ccgccgccat gaaggcgatg acatgggtat tgtcgaaccc
     1501 cggagtgtat catacagccg gcaggacggg acgctggttc atgctgaacg tgccgttcac
     1561 ggtaaagaac aaactcaatg cctggtatct ccagcgtgaa ttgccggagc ctccaaaaca
     1621 atcctttggg gaatggtata aaaaaaataa aagatcatga gcagccgcga taagatcctt
     1681 gacaccgtca aagcaaacca gcccgaaccc cgcgagctgc cgccccacca caccgggccc
     1741 gccgcactat ccgatctcac cccccaattc atcaaggtcc tcgaatccat cggcggcgcc
     1801 gccttcctgg tatccggtta cgatcgcgtc gccatgatcc tccgcgaaca attccccgat
     1861 gccaatcgca tcattacgag ctgcccccaa ctcaccgcct tcgccgccgc ctcatccgcc
     1921 gccgccgcgc tttccgccgc tactccggct gacccccact ccttcgaaaa cgtcgacctc
     1981 gccatcctcc ccgcacactt cggcgtagcc gagaacggcg cctgctggct caccgaagca
     2041 cagatgctcg accgcgtcct ccc
//
LOCUS       DATGFG010000099         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_446731, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000099 DATGFG010000000
VERSION     DATGFG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446731"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VKQ52_01870"
     CDS             <1..456
                     /locus_tag="VKQ52_01870"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ85951.1"
                     /translation="VLPANASVNDNLDFQYDFRNMYSTLLSNWLCVDDTGMENIMFKK
                     YDALPIVNDSACHLGSATPTLAGNTLLSNYPNPFRNTTTLFFKSSGGRVTIRLADTSG
                     QWLQTLVDTDYPAGDYSIVFDGSRLSNGIYYAHFQNMSAHQVRAMSRIR"
     gene            complement(512..1786)
                     /locus_tag="VKQ52_01875"
     CDS             complement(512..1786)
                     /locus_tag="VKQ52_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HLZ85952.1"
                     /translation="MVSRRGFLKGSGLALFGVGLMGGIPGFMAEAAAREKRFLPYKRK
                     RVLVCIFQRGAMDGLMAVTPFSDTYLQAARPTLSLPAVASGRGSGLIDLDGRWGLHPN
                     MKAFEPLFRDGRLAIVHGIGSPNNTRSHFDAQDYMESGTPFNKGTASGWLNRAVGLLG
                     HDALTPFTAVSLTSALPRSFYGDHPSVAISNLQDFAIQLRGNPAAANVAAKSFEELYD
                     QTSAGLLHDAGKESFEAMKILQQADLRNYRAAGGAVYPNSPLGNSLRQIAQLIKMDVG
                     LEVAFTESNGWDTHFNQGTDNGIFARNVTDLSNSISAFWRDLDVYQDDVTVMTMTEFG
                     RTVHQNGTGGTDHGRGSCNFILGNNVAGGRVHGDVPMLAAENLEDGRDLPVTTDFRSV
                     FSAVADEHLKIADDKVLFPDWAGKKAGVMKPA"
     gene            complement(1797..>3661)
                     /locus_tag="VKQ52_01880"
     CDS             complement(1797..>3661)
                     /locus_tag="VKQ52_01880"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HLZ85953.1"
                     /translation="AVVAIGGWAFSRGGTPPGYRFPDANERRESYPFPWHQAGLSERQ
                     AAAHLVSRFTYGATPGEVDAVVQMGLENWFARQLEGSLPDDSLEGRLSRYDALELSNA
                     EVANAYPRNGQVVRMAIRDGVIDKDSVKVDRKEYREVIQNYMQQKGLKREQDLFRQFF
                     CQKILRATYSGNQLQEVMTDFWFNHFNVSITKNDCAEFIPAYERDVIRPNALGRFTEL
                     LLKTAKSPAMLYYLDNFISSAPLPENQQTMNVSMPMDSTGQAVLKARRARGLNENYAR
                     EVMELHTLGVDGGYTQQDVTEAARVLTGWGVYPMGEFARGGGAAQLNRFTDAQIAHRG
                     FVHDGDFFFNANRHDKGEKVVLGHVFPAGGGYEEAVTLLHMLAHQPATAKFVCRKIAV
                     RFVSDEPPASLVDKMTRTFLDKNGDIRQVLLTMVESPEFWSPSALREKTKSPFELAIG
                     AVRGLNGDIRQPMPLYNWVTRMGERKYYYQAPTGFPDRGTYWINTGSLLNRMNFGLAL
                     AGGRVPGVKIDLLALNGGHEPESAEAALAIYGKIILPERDLAATMKRLAPMLNDKGLA
                     EKVNQAAEPGVGAMADAPGKTMGDVSGSVRQDESDQLLAQAVGILIGSPEFQRR"
BASE COUNT          808 a         1334 c          813 g          706 t
ORIGIN      
        1 gtcctgcccg ccaacgcctc ggttaacgac aacctcgatt tccaatatga tttccggaat
       61 atgtactcca ccctgctaag caattggctt tgcgtagatg acacgggaat ggagaacatc
      121 atgttcaaga agtacgacgc gctgcccatc gtaaacgaca gcgcctgcca cctcggctcc
      181 gccaccccca ccctcgccgg caataccctc ctgtccaatt atcccaaccc cttccgcaat
      241 acgacaaccc tcttcttcaa atcctccggc ggccgcgtca ccatccgcct cgccgatacc
      301 agcggtcaat ggctgcaaac cctggtggat accgactacc ccgccggtga ttattccatc
      361 gtattcgacg gcagccgcct gagtaacggc atctattatg cgcacttcca gaacatgagc
      421 gcccaccagg taagggccat gtcccggata agataagtcc cgggctgcca cccgacaccc
      481 cgacagccac cccgaagccc ggccggcccc tctacgccgg tttcatcacg cctgccttct
      541 tccccgccca gtcggggaac aacaccttgt catcggcgat cttcagatgc tcatccgcca
      601 ccgcgctgaa cacactccgg aaatccgtcg tcaccggcag atcacggcca tcctcgagat
      661 tctccgccgc aagcatgggg acatccccat gcaccctgcc cccggcaaca ttattcccaa
      721 gaataaaatt gcaactgcca cgcccatgat cggtcccgcc cgtcccattc tgatgcaccg
      781 tccgcccaaa ctccgtcatc gtcatcaccg tcacatcatc ctggtaaaca tcgagatcac
      841 gccagaatgc gctaatacta ttgctcagat cggtcacatt gcgggcaaaa atgccattgt
      901 ccgtgccctg gttgaaatgc gtatcccaac cattggattc cgtgaacgcc acctccaacc
      961 cgacatccat cttgatcagc tgcgctatct gccggagcga attcccaagc ggactgttgg
     1021 gatacacggc gccacccgcc gcccggtaat tccgcagatc ggcctgttgc aggatcttca
     1081 tcgcctcgaa gctctccttc cccgcgtcat gcaacaaccc cgccgaggtc tgatcataga
     1141 gttcctcaaa actcttcgcc gccacattcg cggcagcagg atttccccgc agctggatcg
     1201 caaaatcctg caggttgctg atggccacgg acggatgatc cccataaaaa gaacgcggca
     1261 acgccgacgt aagactcacc gccgtaaagg gcgtcagcgc atcatgccca agcaaaccca
     1321 cggccctgtt caaccagccg ctggccgtcc ctttattgaa cggcgtgccg gactccatat
     1381 aatcctgcgc atcgaaatga ctgcgggtgt tattgggcga gccgatccca tgaacgatcg
     1441 ccagacgacc atcccggaac aacggctcga atgccttcat attgggatgc aaaccccaac
     1501 gaccatcgag atcgataagg ccgctcccac gcccgctggc aacggccggc aacgacagcg
     1561 tcggccgcgc cgcctgcaga taggtatccg aaaaaggcgt caccgccatc aacccatcca
     1621 tcgccccccg ctggaagata cataccagca cacgcttccg cttatagggc aaaaatcgct
     1681 tctccctggc cgcggcctcc gccataaaac ccgggatacc acccatcagg cctacgccaa
     1741 aaagcgccag accactcccc ttcaaaaaac ctcttctcga taccataact gtgattttat
     1801 cttctttgaa attccggtga cccgataagg atgcctaccg cctgcgccag cagttggtcg
     1861 gactcatcct gccggacact cccggacaca tctcccatcg tcttcccggg cgcatcggcc
     1921 atcgccccaa ctcctggttc cgccgcctgg ttcaccttct ccgcaagacc cttgtcattc
     1981 agcatcggcg ccagccgctt catcgtcgcg gcaagatcac gctcgggcaa aatgatcttg
     2041 ccatagatcg ccagcgccgc ttccgcactc tccggctcat gcccaccgtt caatgccagc
     2101 agatcgatct tcacccccgg aaccctccca cctgccagcg ccagtccaaa attcatccgg
     2161 ttcaacaagg aacccgtatt gatccagtac gttccccgat cgggaaagcc cgtcggcgcc
     2221 tggtaataat atttccgctc tcccatccgc gtcacccaat tgtacagggg catcggctgc
     2281 cggatatccc cattcaatcc acgcaccgcg ccgatggcca gctcgaacgg cgacttcgtc
     2341 ttctcccgca gggccgaagg actccagaat tccggcgact ccaccatcgt cagcaacacc
     2401 tgccggatat ccccattctt atccaaaaag gtcctggtca ttttatccac aagcgacgcc
     2461 ggcggctcat cactgacaaa tcgcaccgcg atcttccggc agacgaactt cgccgtagcg
     2521 ggctgatgcg ccagcatatg caacagggtg accgcttcct catagccgcc acccgcggga
     2581 aagacatgcc ccaacaccac cttctctcct ttgtcatgcc ggttggcatt gaaaaagaaa
     2641 tccccgtcat gcacaaaacc ccggtgcgcg atctgcgcat ccgtaaaccg gttcagctgc
     2701 gctgcgcccc caccgcgggc aaactcaccc atcgggtaaa caccccaacc cgtcaacacc
     2761 cgcgccgcct cggtgacatc ctgctgcgta taaccgccat cgaccccaag cgtatgcaac
     2821 tccatcacct cccgcgcata gttctcgttg agcccccgcg ccctccgcgc cttcagcacc
     2881 gcctgccccg tggaatccat cggcattgat acattcatcg tttgctggtt ctccggcaac
     2941 ggggcgctgc tgatgaaatt atcgagataa tacaacatcg ccggcgactt ggccgtcttc
     3001 agcaataatt cagtaaagcg gccaagcgca ttcggccgga tcacatcccg ctcgtatgcc
     3061 ggaataaatt ccgcacaatc attcttggta atggagacat taaaatggtt gaaccagaaa
     3121 tccgtcatca cttcctgcag ctggttcccc gaataggtag cccggaggat cttctgacaa
     3181 aagaactgcc ggaaaagatc ctgctcccgc ttcaacccct tctgctgcat ataattttgt
     3241 atcacctccc ggtattcctt gcggtcgact ttcaccgaat ccttatcgat caccccatcc
     3301 cggatcgcca tccgcaccac ttgcccgttg cggggatagg cgttcgccac ctccgcattg
     3361 ctcagctcga gggcatcata ccggctcaac cgcccctcga gcgagtcatc cggcagcgac
     3421 ccttccagct gccgggcaaa ccagttctcc agccccatct gcaccaccgc atccacctcg
     3481 ccgggcgtcg ccccatacgt gaaccggctc accaggtgcg ccgccgcctg ccgctccgaa
     3541 agcccggcct gatgccaggg gaatgggtag gattcccgtc tttcattagc gtccggaaac
     3601 cgataacccg gcggagtccc cccacgggaa aacgcccatc cccctattgc cacaacggca
     3661 a
//
LOCUS       DATGFG010000100         6715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1009396, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000100 DATGFG010000000
VERSION     DATGFG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009396"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VKQ52_01885"
     CDS             <1..242
                     /locus_tag="VKQ52_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HLZ85954.1"
                     /translation="RDQEFTIKGVERKTLVAQLEALILMITSTLNALTPEQMEADYPI
                     LFDGEKRPVNYLVVQLLAHLNYHLGQVNYLRRILE"
     gene            506..1315
                     /locus_tag="VKQ52_01890"
     CDS             506..1315
                     /locus_tag="VKQ52_01890"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HLZ85955.1"
                     /translation="MRKITDKDYSLPVIEALSPIESFETGANRPLLIRGVDAAGNKGD
                     YVVKFLGAERMSTEACMRELLAAFIAAQMEIQVVNPVIVNISQDFVDLLKGNSAWQYA
                     SKSLGYNYGSEYIKRHSIIIPAQDLNHHQLYYAQTIFAFDVTIQNPDRTIEKPNMLTE
                     GTEIVIYDHELAFSFVMDIFPNPKPWELRQADLEWIGRHCLLPKIKGKEYDFEEFSNR
                     FNNLDENFWAIARSLIPKEWLSDQFDRIKQHFSAICNNKDAFIIELKKLMS"
     gene            1312..2154
                     /locus_tag="VKQ52_01895"
     CDS             1312..2154
                     /locus_tag="VKQ52_01895"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HLZ85956.1"
                     /translation="MITIQYQILRYLPDRVSGEFVNLGIVAFDSSSRSLKSKFINRIG
                     NISSFFPNINSRYLIKSIKTIQEELEKFSARLKSEFIFTQVSSIEEVTRATLPKDDSS
                     LVFSEIKKTLDVSVDATVSDLFPRLVSAHLMDEEDDEIRRDKEVWTKVYKKHFDDYGI
                     SSHLSSHRVKTKNDELEFDKAWKNGVWNCFESVSFNLTRTDSIKNKVYKWVGKLDELG
                     SAHESMHIYLLSVLPTDNSELDRFITKKIKEKATDNVRIELVSEDEIERIAKKIKKEI
                     DKHS"
     gene            complement(2335..4383)
                     /gene="fusA"
                     /locus_tag="VKQ52_01900"
     CDS             complement(2335..4383)
                     /gene="fusA"
                     /locus_tag="VKQ52_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLZ85957.1"
                     /translation="MKRLYRRRNIGIMAHVDAGKTTVTERLLYYTGLTHQLGNVDDGN
                     TIMDTDAQEAQRGITISSAAITTHWNYAGSRYPITIIDTPGHVDFTAEVERSLRVLDG
                     AVAVFCARSGVQSQSETVWHQADRYAIPRLVLINKMDRQGADFQRVVTEIRDKLQANA
                     LPVQLPIGAEDAFRGVIDLVTLQAFIWDSEDGKAYEMTDIPEALLPSAQQARERLLEE
                     LALVEVRLFEQYALDPAGITVEAIRDALRRATIDRQIVPVFCAAAYRNKGVQPVLDAI
                     VQYLPAPEDMPFIDATGAAAGPGSPFAGLVFKIVTNDYTGKLCMVRVYAGTLQAGDSV
                     WNSRTGKKVRVNRLLRVMADRYEPVDRLEAGDIGALLGVKETRTGDTLSDPDHPMTLE
                     AMNFPEPMIGYAIEARSAKESVKLGEALSKLLDEDPTLAVEVDTASGQTILKGMGELH
                     LDIVLEKLRSDHQVEVNRGQPQIAYKEVFTRSVSQKATFKKQNGGLGNFAEIEFELSP
                     RPDGLPGLEFLDEIRGGAIPREFIASVQKGFATAMHTGVLAGYPVESMRVRLFDGDIH
                     DKDSHTLDFEQVAQIGFKAAAAQAGAKLLEPIMCVDVTLPEEYTGSVTGDLTRRRGMI
                     TSVERQAAAQRIQAAVPLSELFGYITALRTLTAGRASASMRFERYAIAPEQVSKAMIA
                     "
     gene            4902..5240
                     /locus_tag="VKQ52_01905"
     CDS             4902..5240
                     /locus_tag="VKQ52_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HLZ85958.1"
                     /translation="MSDIQIITDALVKFRDDRQWAQFHDSKNLALAISIEAAELNELF
                     LWKEGAGAEEVDKTRLKEELADIFTFCFLLADKHKLDVKEIILDKIRLNEKKYPVEKA
                     RGTAKKYNEL"
     gene            5264..>6715
                     /locus_tag="VKQ52_01910"
     CDS             5264..>6715
                     /locus_tag="VKQ52_01910"
                     /inference="COORDINATES: protein motif:HMM:NF021375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA helicase domain-containing protein"
                     /protein_id="HLZ85959.1"
                     /translation="MIVYQKNKEGFSNDVLTNDIDGIIIRAIKSSTGKNIAASEVNAF
                     RQSLHYMDTVLHDKDIPDDCGISIEYHIPCTSKRIDFLITGSDGDKENLIIVELKGWS
                     EAWLTERDGMVDTRFQGRVSRTTHPSYQAWSYAALLQAFNATIEEESIYVQPCAYLHN
                     YAPDEVLTNEFYNYYTDKAPLFLKPDAVQLRAFIKRFMKYGDRKNLIIRIENGKIRPS
                     KSLADSLRSIMDGNKEFIMIDDQKAVYEEALYLAAHSNEKTKNVLIVEGGPGTGKSVV
                     AINLLVELNRRGMMAQYVTKTSAPREVYFDKLSPDKKMPELRNLFVSSGSFISAPKNS
                     VAALIVDEAHRLTEKTGFLKMGDNQVKEIINTAPFSVFFIDEDQRVHISDYAESWIIE
                     EWAKKLGAGVTKLSLSSQFRCCGSDGYLAWLDNLLQIRETANETFQSADFKYDFRVVD
                     SPTELRDLIFEKNKINNKARLVAGYCWDWKSAKN"
BASE COUNT         1754 a         1692 c         1701 g         1568 t
ORIGIN      
        1 accgcgacca ggagtttaca ataaaagggg ttgaaaggaa aaccctcgtt gcccagctgg
       61 aagcactgat cctgatgatc accagtacac tgaacgcact gacgccggag caaatggagg
      121 ccgattaccc catactgttc gacggtgaga aaaggccggt caactacttg gtggtgcagt
      181 tgttggccca tttaaactac catctggggc aggtgaatta tctccgccgc attctagaat
      241 agccggcaac gaaaagacct cctcccaaaa cgccgtgata tatccgaaca gctccgacga
      301 gggcgccgcc gcttgaattc gctgcgcggc tgcctgttgt tccactgatg taatcatacc
      361 gcgccggccg gtgagatccc ctgtcaccga tccggtatat tcttattgcc ggtagacgag
      421 tacgaaagcc aatcgctagg taagtattaa aaaatcgacg cgtttgattt cgttatattt
      481 gtttaaccca atgaaaaaat gctgtatgag gaaaatcact gataaagact attcgttacc
      541 ggtaatagag gcattaagcc ctatcgaatc ttttgagacc ggggcgaatc gacccctttt
      601 aataaggggt gtagatgctg ccggtaacaa gggtgattat gtagtaaaat tcctaggtgc
      661 cgagcgcatg tccaccgaag cctgtatgcg tgaattattg gccgctttta ttgcggctca
      721 aatggaaatc caggtggtta atccagttat tgtcaatata tcccaagatt ttgtcgatct
      781 cctaaaaggg aatagcgcgt ggcaatatgc aagtaagagc ctggggtaca actacggttc
      841 ggagtatata aaaagacact ctataattat accggctcag gatctgaacc accaccagct
      901 atactatgct cagaccattt ttgccttcga tgtaactatt caaaacccag atagaactat
      961 cgaaaaacct aatatgctta cggaaggaac ggagattgtg atctacgacc atgaactggc
     1021 cttcagcttc gtgatggata tatttcccaa cccaaaacca tgggaactgc ggcaagctga
     1081 cctggaatgg atcggccggc actgcctctt gcccaaaatc aagggcaaag agtacgattt
     1141 cgaggaattt tcaaacaggt ttaataactt agatgaaaat ttctgggcaa tcgccaggag
     1201 cttgattccc aaggagtggc tttcagacca gtttgaccgg atcaaacaac atttctccgc
     1261 aatttgtaat aataaagacg cgtttatcat agaacttaaa aaattgatgt catgatcacc
     1321 attcagtatc agattttacg atatctgccg gacagagtat ccggggaatt tgtgaatctt
     1381 ggcattgtcg cttttgattc ctcttcccgg tcgctaaaat ccaaattcat taacagaatt
     1441 gggaatattt ctagtttctt tcccaatatt aatagcaggt atcttataaa atccataaaa
     1501 acgatccagg aggaactgga gaaattttct gccaggctca aatctgaatt tatatttacc
     1561 caagttagtt ctatcgaaga agtaacaagg gccacattgc caaaggacga tagctccctg
     1621 gtcttttcag aaattaaaaa gacacttgac gtttcagtcg atgccaccgt aagtgaccta
     1681 tttccacgct tggtaagtgc acaccttatg gatgaagagg atgatgaaat tagacgagac
     1741 aaggaagttt ggaccaaggt ttacaaaaag catttcgatg actatgggat ttccagccat
     1801 ttatcctctc atagagtaaa aacaaagaac gacgagctgg aatttgataa agcatggaaa
     1861 aacggcgttt ggaactgctt tgaaagcgtt tcattcaacc tgacccggac agattcaata
     1921 aaaaacaagg tctataaatg ggttggtaaa ttagatgaac ttggcagcgc gcatgaatca
     1981 atgcatattt atcttttaag cgttctccca accgataatt ccgaattgga caggtttatt
     2041 accaagaaaa taaaggagaa agcaacagat aatgttagga ttgagttggt ttcagaagat
     2101 gaaattgagc gtattgccaa gaaaattaaa aaagaaatcg acaagcattc gtaagtggct
     2161 gggaccactt acggacatcg gattctcctg gttcagggtc cgcttgtcga tatcgatgcc
     2221 cattcggtgc cccttagcca ctggttctcc ggcaagaaaa gacctccccc gaagggaagg
     2281 tcttggttat taacgcggtc gccggcccgt gctcgggctt tctccggtcc atattcaggc
     2341 aatcatcgcc ttactcactt gttccggcgc tatcgcatat cgctcgaatc gcatcgaagc
     2401 cgacgccctt ccggcggtca aggtccgcag cgccgtgata tacccgaaca gctccgacaa
     2461 gggcaccgcc gcttgaattc gctgcgcggc cgcctgtcgc tccactgacg taatcatacc
     2521 ccgccggcgg gtgaggtccc ctgtcaccga tccggtatat tcttccggaa gggtaacatc
     2581 cacacacatg atcggttcca gcaatttggc accggcctgt gcggccgctg ctttgaaacc
     2641 gatctgcgcc acctgttcga aatccagggt gtgggaatcc ttgtcgtgga tatcgccgtc
     2701 gaacagtcgc acccgcatgg attcgaccgg gtaacccgcc aacacacccg tatgcatcgc
     2761 cgtcgcaaaa cctttttgca cagaggcaat gaactcccgg gggatggctc cgccacgaat
     2821 ctcgtccaga aactccaatc ccggcagtcc gtccggtcgc ggcgaaagtt cgaactcgat
     2881 ctccgcgaag ttccccaatc cgccattctg ctttttaaag gtcgccttct gcgacacgct
     2941 gcgcgtgaag acttccttat aggcgatctg cggctgacca cggttcactt ccacctggtg
     3001 atcgctacgc agcttctcca acacgatgtc caggtgcagc tcgcccatgc ctttcaggat
     3061 ggtctgtccc gacgccgtgt cgacttccac ggccagggtc gggtcctcgt cgagcagctt
     3121 cgacagcgcc tcgcccagtt tcacactttc ctttgccgat cgggcttcga tggcgtaacc
     3181 gatcatgggc tcggggaagt tcatcgcttc caatgtcatg ggatggtcag ggtccgacag
     3241 ggtgtccccc gttcgggttt ccttcacgcc caggagcgcg ccgatgtccc cggcttcgag
     3301 acgatctacc ggttcatacc gatccgccat gactcgcagc aaccggttca cccggacctt
     3361 cttacccgtc cggctattcc acacactgtc gccagcctgc agggtgccgg catagacgcg
     3421 taccatgcat agcttgcccg tataatcgtt ggtcacgatc ttgaacacta ggcccgcgaa
     3481 ggggcttccc ggtccggccg cggctccggt ggcgtcaata aaaggcatat cctcgggcgc
     3541 cggaagatat tgcacgatcg catccagcac aggctgcaca cctttgttcc ggtaagccgc
     3601 ggcacagaaa acggggacga tttgacgatc gatggtagcc cttcgcaaag catcccgtat
     3661 cgcttccacg gtaatccccg caggatccag cgcatactgc tcgaatagtc gcacctcgac
     3721 aagcgctaat tcttccagga gtcgctcccg tgcctgttgg gccgaaggca gcagcgcttc
     3781 cgggatgtcg gtcatctcat acgccttgcc atcctcacta tcccaaataa aggcttgcag
     3841 ggtgacgagg tcgataacac ccctgaacgc atcctctgcg ccgatgggta gctgcacggg
     3901 gagggcgttc gcctgcagct tatcgcggat ctccgtgacc acacgttgga aatcggcgcc
     3961 ttgccggtcc attttattga tgaggaccag gcgcgggatg gcataccgat cggcctgatg
     4021 ccataccgtc tcggactgtg actggacccc cgagcgcgcg caaaaaacgg cgacggcccc
     4081 gtcgagcacc cgcagcgatc gctctacctc ggcggtaaag tctacgtgac cgggggtgtc
     4141 gataatcgtg atcggatacc ggctgccggc gtagttccaa tgcgtcgtga tggccgcgga
     4201 ggaaatggtg atgccccgtt gcgcctcctg ggcgtcggtg tccatgatgg tattgccatc
     4261 gtcgacattt cccagttggt gtgtcaggcc tgtgtaataa aggaggcgtt cggtcacggt
     4321 cgtcttgccc gcgtctacgt gcgccatgat gccaatattt cgtcgtcgat atagtcgttt
     4381 cataaatctt gcattgataa atgtataaaa aaacctcccg ctcggggagg tcgcagcaat
     4441 aggatgccag gccacctacc ctatgggtat gggtgaacta taaaatcgcc cttgaacgcg
     4501 ctaataacgt gttgcatatt gctgctttga aaatgatgac ttggtctaac tatgggatgg
     4561 aaggggaggc aaactaacct ctcgaaggag acgggaatgc aattgcctcc ccgtctgaaa
     4621 taaagacgag ccgggtactg gcaaggattg cctggacggc gctgacggcc ggtgtaccaa
     4681 ggtgatatgt gaaggttctg agcatgaagg tggctgttag ccgggtgcaa aggtagaaag
     4741 aaaataatac aaagcaaatc tttgggcaaa atccgcagaa tagacccctt gaaaccaggg
     4801 caccgcccct tattttttcg tcttagaaag ctagaagctc ctcgatctac aaaagcttgg
     4861 caatattccg atttttgaat accttaaacc aaatagctat catgtctgac attcaaataa
     4921 ttacagatgc attagtaaaa ttccgggacg accggcagtg ggcgcaattt cacgactcga
     4981 agaacctggc gctggcgatc tccatcgagg ccgccgagtt gaatgagttg ttcttatgga
     5041 aagagggtgc cggcgcggaa gaggtagata agacccggtt gaaagaggaa ctggcggaca
     5101 tttttacatt ctgtttcttg ctggccgaca aacacaaatt ggatgtcaag gagatcatcc
     5161 tggacaagat ccggcttaac gaaaaaaaat accccgttga aaaggcccgg ggcaccgcaa
     5221 aaaaatacaa cgaactctaa aatctgcagc tgaaatctta tcaatgatcg tctaccaaaa
     5281 gaacaaggaa ggatttagca acgatgtcct gacaaacgat atagatggca tcatcatcag
     5341 ggccatcaaa tcctctaccg gcaagaatat tgctgccagt gaggtgaatg cattccggca
     5401 atccctgcac tacatggata ccgtccttca cgacaaagac attccggatg actgtggcat
     5461 ctcgatagaa taccatattc cctgtacttc gaaacggatc gattttctta tcaccggctc
     5521 tgacggcgat aaagaaaacc tgatcatagt ggagttgaaa ggctggtcgg aagcctggtt
     5581 gacggaaaga gatggaatgg tcgatactcg gtttcaaggg agggtcagcc ggactacgca
     5641 tccctcctat caggcatggt cctatgcggc attgctacaa gcctttaacg cgaccatcga
     5701 ggaagaatct atatatgtcc agccatgcgc ctatctgcac aactatgcgc cggacgaggt
     5761 actgaccaat gaattttaca actactatac ggataaggct ccgctcttcc tcaaaccgga
     5821 cgctgtccag ttgcgggcgt tcatcaagcg attcatgaaa tatggggata gaaagaattt
     5881 gatcatccgg atcgagaacg ggaagatcag gccctcgaaa agcctggccg atagtcttcg
     5941 gtcgattatg gatggaaaca aagagtttat catgatcgac gaccagaagg cggtgtatga
     6001 ggaagccctc tacctggcgg cacattccaa tgaaaagact aagaatgtcc tcatagtgga
     6061 gggcggccct ggtaccggta aatcggtagt tgccatcaat ctcctggtgg agctcaaccg
     6121 tcggggcatg atggcgcaat atgttacaaa gacaagcgcc ccccgggaag tctattttga
     6181 caagttaagt ccggataaaa agatgccgga actgcggaac ctgtttgtca gttcgggctc
     6241 cttcatatcg gcgccgaaaa actcggttgc cgcgcttatt gtcgatgagg cccatcgcct
     6301 gacggaaaag accgggtttt tgaagatggg ggacaaccag gtgaaggaga tcattaacac
     6361 ggcgccgttt tcggtgtttt tcatcgatga ggatcagcgg gtgcatatca gtgattatgc
     6421 ggaaagctgg atcatcgagg aatgggcaaa aaagcttggg gctggcgtta ccaaactcag
     6481 cttgtcctcg caattccgtt gctgcggatc ggatggctat ctggcctggt tggacaatct
     6541 tctccagatc cgcgagacgg ccaatgagac cttccaatca gccgatttca aatacgattt
     6601 ccgggttgtg gatagcccga cggaacttcg ggatctgatc ttcgaaaaaa acaaaataaa
     6661 caacaaggca aggctggtgg cgggctactg ctgggattgg aaaagtgcca agaat
//
LOCUS       DATGFG010000101         5788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_894393, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000101 DATGFG010000000
VERSION     DATGFG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894393"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5337
                     /locus_tag="VKQ52_01915"
     CDS             <1..5337
                     /locus_tag="VKQ52_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ85960.1"
                     /translation="STGKKDTAALRQHITRTLPDYMMPAAFVWLDELPHTTSGKIDKR
                     ALPKPEIKRPELTVLYKAPATKTETILATLWATLLQLDKVGAEDNFFELGGNSLVALK
                     TVAALQQQHGLDLPITKLYQFPTIKAVAAWLDGGQKARIRLPKDHGRRNTGTPGNDDI
                     AIIGMAGRFPGASTIEELWTLLKEGKETVSFFSKEELDASIPAGIRNDPDYVAARGII
                     DGADTFDAGFFGINPRLGELMDPQQRIFLEIAWEALERTGYLPGHYSGSIGVFAGSGN
                     NSYYLNNVIPNKELVDKVGGFQVMTYNEKDYVASRTAYVLNLKGPAVSVHSGCSTSLL
                     AIAQAVDSLRKGQCDIALAGGASITAPIRSGHIYQEGAMLSRDGHCRPFDAEAQGTTF
                     SDGAGIVLLKNRQQAEKDGDTIFALIKGTGVNNDGGGKGSFTAPSAEGQAGAIAMAIA
                     EAGIDASSISYIEAHGTATPLGDPIEIEGLQMAFGEQEKRQYCALGSIKSNMGHLTAA
                     AGVAGLIKTTLALWHRQLPPSINFNSANPAIDFGQTPFFVNTTFRDWQAAGTRRAGVS
                     SFGVGGTNVHVILEEAPQPVIPASTSARPLQLISWSARTPKSREAYGQRLAAFAGDTN
                     ATGDRLADIAYTLHTTRADFAARRFILASDEKDLIRQLQQPEPAPSTAAILDEARADI
                     VFLFPGQGSQQVNMGRELYRQEPVFRQAVDECAQLLAPIMGEDIREILYPQQDTPDTA
                     ARINNTLYAQPALFVIEYALSCLWKSWGITPSAFAGHSIGEFVAAHLAGVFSLKDGLQ
                     LIATRGRMMAEQPGGGMLSIRAPHKSIIPLLPDNLSVAAVNSPELCVVSGPTGAITAF
                     STLLETQGIQNKLLYTSHAFHSAMMDPVVGAFRQLVASIPLNLPRLPIASTATGKWLT
                     DTEATSPDYWAGHLRTTVLFSDAITTLLDNGNRLMLEVGPGKVASVLVRQHRTAKPAI
                     AISSLDAEEGTSQYHAILKALGQLWLNGIRPDWKAFYTGRQHRRLDLPAYAFDRQPAW
                     INPPQAQPQTQHPTIPTPSLPSLPNSTTENLTKPMRTTDLLRKVKEILEDASGIDLQT
                     ADPAAGFVELGLDSLLLTQVALNLKKSFNLPITFRQLNEEYGSLNLLVSYLDATLPPD
                     PVPSQAQPLPQAQAPQQAAPVTYASAQYNGAVIGNNTTVIGLISQQIQLLAQQIALLQ
                     GGTPAPAPAPAAIPTSAPFIATPQALAAPTFTPAPVNGKAKTETDLTAEEIAEHKKPF
                     GATAKIDKQARELDSRQQRFLDDLVSRYTTKTASSKAYTAQHRSYMADPRVVTGFKPQ
                     TKEIVYPIVVNRSKGSRLWDLDGNEYIDALNGFGSNMLGYQPDFIVRAMQDQIEKGYE
                     IGPQHELAGPVSKLICEFTGFDRAALCNTGSEAVLGAMRIARTVTGRSTIVAFAGSYH
                     GIVDEVIVRGSKKLKSYPAAPGIMPEAVQNMLILDYGTDESLRIIKERAHELAAVLVE
                     PVQSRRPEFQPVEFLQQLRKITENAGTCLIFDEVITGFRMHPGGAQALFGVKADLGTY
                     GKVIGAGVSIGVIAGKKLFMNALDGGDWKYGDNSTPEAGVTYFAGTFVRHPLALAASK
                     ASLEYMKAKGPALQQSLTEKVKYLADSCNAICQRHSLPLFVAQFGSLWKLKWKEEILY
                     SELLFTLMRDKGIHILDGFPCFLTEAHSRQDVDTIIEKFGESIDQLIDAGFLQSTNTN
                     PTVLNKNHGTKSEIKVDETPLIPGARLGRDKDGNPAWFINDPDRPGKYLQVN"
     gene            5349..>5788
                     /locus_tag="VKQ52_01920"
     CDS             5349..>5788
                     /locus_tag="VKQ52_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLZ85961.1"
                     /translation="MDHSTNDLLPVEFDPFSGPEILRVLPAIEPQQEIWISCRLGGED
                     ANRSYNESVSLRLNGPFDQPAMEHALRDLIARHEALRSAFSADGRKMIVFRELPANLY
                     FEDLSTRQPDEQQAFIDSFSKTEARTAFDLLTGPLFRMALFRLG"
BASE COUNT         1343 a         2122 c         1385 g          938 t
ORIGIN      
        1 agcaccggga aaaaagatac cgccgcactc cggcaacaca tcacccggac cctgcccgac
       61 tacatgatgc ccgcagcctt tgtctggctc gacgagctcc cccacaccac cagcgggaag
      121 atcgacaaac gcgccctccc aaaacccgag atcaaacgcc cggaactcac cgttctttat
      181 aaggccccgg ccacaaagac cgaaaccatc ctcgccaccc tctgggctac cctcctccaa
      241 ttggacaagg taggcgccga agacaacttc ttcgagctgg gcggcaattc cctcgtagcc
      301 ctgaagaccg tggccgccct gcaacaacaa cacggcctcg acctgcccat taccaaacta
      361 taccaattcc ccaccataaa agccgtagcc gcctggctgg acggcggaca gaaagcccgc
      421 atccgcctgc cgaaagacca cggccggcgc aataccggca cccccggcaa cgacgacatc
      481 gccatcatcg gcatggccgg ccgcttcccc ggagcctcca ccatcgaaga attgtggacc
      541 ctcctgaaag aaggaaaaga gaccgtctcc ttcttctcga aagaagaact ggacgcctcc
      601 atccccgccg gcatccgcaa cgacccggac tatgtcgccg cccgcgggat catcgacggg
      661 gccgacacct tcgacgccgg cttcttcggc atcaaccccc ggctcggcga actgatggac
      721 ccccaacaac ggatcttcct ggaaatagcc tgggaggccc tcgaaagaac cggctacctc
      781 cccggccact attcgggctc catcggcgtc ttcgccggct cgggaaataa cagctactat
      841 ctcaataacg tcatcccaaa taaagaactc gtcgataaag tagggggctt ccaggtgatg
      901 acctacaatg aaaaagatta tgttgccagc cggacggcct atgtcctcaa cctgaaaggc
      961 cccgccgtca gcgtccactc cggctgctcc acctccctgc tggccatcgc ccaggccgtg
     1021 gacagcctcc gcaaaggcca gtgcgatatc gctcttgcag gcggcgcctc catcaccgcc
     1081 cccatccgca gcggccatat ctaccaggaa ggcgcgatgc tcagccgcga cggccactgc
     1141 cgccccttcg acgccgaggc ccagggcacc acctttagcg acggcgccgg catcgtcctg
     1201 ctaaagaaca ggcaacaggc cgaaaaggac ggcgacacca tcttcgccct gataaaaggc
     1261 acgggcgtca acaacgacgg cggcggaaaa ggcagcttca ccgccccaag cgcggaagga
     1321 caggcgggcg ccatcgccat ggccatcgcc gaagccggca tcgacgcctc ctccatcagc
     1381 tacatcgaag cccatggcac cgcaacaccc ctgggcgacc ccatcgagat cgaaggcctc
     1441 cagatggcct tcggcgaaca ggaaaaacga caatattgcg ccctcggctc catcaaaagc
     1501 aatatgggcc acctcaccgc agccgcgggc gtcgcaggcc tcatcaaaac aacactggcg
     1561 ctctggcacc ggcagctgcc cccctccatc aactttaact cggcaaaccc ggccatcgac
     1621 ttcggccaaa cgcccttctt cgtcaacacc accttccgcg attggcaggc cgccggcaca
     1681 agaagggcag gcgtcagctc ctttggcgta ggcgggacaa atgtccatgt catcctcgaa
     1741 gaagcgccgc agcccgtcat tcccgcttct acatccgcca ggccccttca actcatttcc
     1801 tggtccgcca ggacaccaaa aagccgcgaa gcctacggcc agcgactcgc cgcctttgcc
     1861 ggcgacacca atgccaccgg cgatcgcctc gcggacatcg cctataccct ccataccacc
     1921 cgcgccgact tcgccgcccg ccgattcatc ctggccagtg acgaaaaaga cctgatccgg
     1981 caactgcaac aacccgaacc ggccccgtca accgccgcca tcctcgacga agcacgcgcc
     2041 gatatcgttt tcctcttccc gggacaaggc tcccaacagg tgaacatggg tcgtgaactc
     2101 taccggcagg aacccgtctt ccggcaggca gtagacgaat gcgcccaatt gctcgccccc
     2161 atcatgggag aagacatccg tgagatcctc tacccgcaac aggatacccc ggacaccgcc
     2221 gcccgcatca acaacaccct ctacgcccaa cccgctctct tcgtcataga atacgccctc
     2281 tcctgcctct ggaaaagctg ggggatcacc ccctccgcct tcgccggaca cagcatcggg
     2341 gaattcgtcg ccgcccacct cgccggcgtc ttcagtctga aggacggcct tcagctgatc
     2401 gctacccgcg gccgcatgat ggcggaacaa cccggcggcg gcatgctcag catccgcgcc
     2461 ccgcacaaaa gcatcatccc cctgctaccc gacaatctct cggttgctgc cgtcaacagt
     2521 cccgaactct gcgtggtatc cggtcccacc ggcgcgatca ccgccttttc caccctgctc
     2581 gaaacccagg gcatccagaa taaactgctg tacaccagcc atgccttcca ttcggccatg
     2641 atggaccccg tcgtcggagc cttccgccaa ctggtagcat ccatcccgct aaacctgccc
     2701 cggctgccca tcgcatccac cgccaccggt aaatggctca ccgacacgga agccaccagc
     2761 ccggactact gggcgggtca cctgcggacg accgttctct tctccgacgc catcacaacc
     2821 ctgctggaca acggcaacag gctgatgctc gaggtcggcc ccggcaaggt ggcctccgtc
     2881 ctcgtccgcc agcaccgcac cgccaaaccc gccatcgcca tcagcagcct cgacgccgaa
     2941 gaaggcacct cccagtacca cgccatcctg aaggccctcg gtcagctctg gctcaacggc
     3001 atccggcccg actggaaagc gttttacacc ggccggcaac accgccgcct ggacctgccc
     3061 gcctacgcct tcgaccggca acccgcctgg ataaacccgc cacaggccca accacaaacc
     3121 caacatccaa ccataccaac accatcgctt ccgtccctac ccaactctac aaccgaaaat
     3181 ttgactaaac ccatgcgtac aacagacctt ctccggaaag tgaaagagat cctggaagat
     3241 gcttccggaa tcgatttaca aaccgccgac ccggccgccg gcttcgtcga gctaggactg
     3301 gactccctcc tgctcaccca ggtggccctc aacctcaaaa aaagcttcaa cctgcccatc
     3361 accttccggc agctgaacga agaatacggc agcctcaacc tcctcgtctc ctatctcgac
     3421 gccaccctgc cccccgaccc ggtaccatca caggcccaac ccctgccaca ggcccaggcg
     3481 ccccaacagg cggccccggt cacctatgct tcggcgcaat acaacggcgc cgtcatcggt
     3541 aacaatacca cggtgatcgg cctcatctcc cagcagatcc aattgctggc ccaacaaata
     3601 gccctgctcc agggcggcac acccgcaccg gcgccggccc cggcggccat acccacatcg
     3661 gcccccttca tcgccacgcc acaggccctg gccgccccca cctttacacc cgcgcccgta
     3721 aacggaaagg caaagacgga aaccgacctc acggccgaag aaatagccga acataaaaaa
     3781 ccctttggcg ccaccgcaaa gatcgataaa caggcccgcg aactcgattc ccgccagcag
     3841 cgcttcctcg acgacctggt aagccgctat acgacaaaga ccgcatccag caaagcctac
     3901 accgcccagc atcgctccta tatggccgat ccccgggtgg tcaccggctt caaaccccag
     3961 acaaaagaga tcgtctaccc catcgtcgtc aaccgctcga aaggcagccg cctctgggac
     4021 ctcgatggca acgaatacat cgacgccctc aacggctttg gctccaatat gctcggctac
     4081 caacccgatt tcatcgtcag ggcgatgcag gaccagatcg aaaaaggcta tgagatcggc
     4141 ccccagcacg agctggccgg ccccgttagc aaactcatct gcgaattcac cggcttcgac
     4201 cgcgccgccc tctgtaacac cggctccgaa gccgtcctgg gcgccatgcg aatagcccga
     4261 acggtcaccg gccgcagcac catcgtcgcc ttcgcaggct cctatcacgg catcgtcgat
     4321 gaggtcatcg tccgtggatc gaaaaaattg aagtcctacc cggccgcccc gggcattatg
     4381 ccggaggcgg tacaaaatat gcttatcctt gattatggga cggacgaaag cctccgtatc
     4441 ataaaagaac gcgcccacga actggccgcc gtcctcgtcg agcccgtcca aagccgtcgc
     4501 cccgaattcc aacctgtgga atttctgcaa caactgcgta agatcacgga gaatgcgggc
     4561 acatgcctca tctttgacga agtgatcacc ggtttccgga tgcaccccgg cggcgcccag
     4621 gccctcttcg gcgtaaaggc cgacctggga acctatggca aggtcatcgg cgccggcgtc
     4681 tcgatcgggg tcatcgcagg taaaaaactg tttatgaatg cattagacgg cggagactgg
     4741 aaatatggcg acaactccac accggaagcg ggagtaacgt attttgcagg tacctttgta
     4801 cgacacccgc tggcgctggc cgcctcaaaa gcctccctcg aatacatgaa ggcgaaaggc
     4861 ccggccctcc agcagtccct tacagaaaag gtaaaatacc tggccgattc ctgcaatgcc
     4921 atctgccagc gacatagcct gccgctgttc gtagcacagt ttggttctct ctggaaactc
     4981 aagtggaaag aagagattct ctatagcgaa ctgctcttca ccctcatgcg ggataaaggc
     5041 atccatatcc tcgatggttt cccctgtttc ctcactgaag cgcatagccg ccaggatgtg
     5101 gacaccatca tcgaaaaatt cggagaaagc atcgaccagc tcatcgacgc cggctttctc
     5161 caaagcacca acaccaatcc aacggtcctc aataaaaacc acgggacgaa aagcgaaata
     5221 aaagtcgacg aaacaccgtt gattcccggc gcaagattag gccgggacaa agacggtaac
     5281 cccgcctggt ttataaacga tcctgatcgc cccgggaagt atttgcaagt gaactaaaac
     5341 gatgacaatt ggaccactct actaacgacc ttttgccggt tgaatttgac cccttctccg
     5401 gacctgagat ccttcgggta ttgcctgcca ttgagccgca acaggagatc tggatctcct
     5461 gccgcctcgg cggcgaggac gccaaccgtt cttacaacga atcggtatcc ctccgcctga
     5521 acggcccctt cgaccagccg gcgatggagc acgccctccg ggatcttatc gcccgccatg
     5581 aagccttgcg ctcggccttc agcgccgacg gccgcaaaat gatcgtcttc cgtgaactgc
     5641 cggccaacct gtatttcgaa gacctctcca cgcgccagcc cgacgaacaa caggccttta
     5701 tcgacagctt ctcgaaaaca gaagcccgga cggccttcga tctgctgacc ggccccctct
     5761 tccggatggc cctgttccgg ctggggaa
//
LOCUS       DATGFG010000102         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1236395, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000102 DATGFG010000000
VERSION     DATGFG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236395"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VKQ52_01925"
     CDS             complement(<1..460)
                     /locus_tag="VKQ52_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85962.1"
                     /translation="MKKACLFIALLTQLGLHAQKIIDISYTQDNQGRYIFTANNKAYC
                     TYVLHIDFPTLTNLNSDHPLPYDVELKPGTSRLLVLTSLDKTKDTRINYKSGNRKGLL
                     NPVVNPNFTYLLPTTPGQETQAYRIPNTKGAASVTGGQDSGYSVRLRAKPG"
     gene            565..2490
                     /locus_tag="VKQ52_01930"
     CDS             565..2490
                     /locus_tag="VKQ52_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HLZ85963.1"
                     /translation="MPAIKLLSAPALLIAAFGFASCNQGASTASGGAESSVTKTADGY
                     TYRDLNKNGKLDVYEDGRQPVDARVEDLLKQMSLEEKAGLLFINGARVNDDGSVGDKP
                     AKGMFAFAPNALKLVQQQKMNHFNLWAVPSPAALAKWYNSMQRYLQDSTRLGIPMTIA
                     SDPRNHFASNVFAMSANTFSQWCEPLGLGAIGDSVLTRQFADVSRREYLAVGIREALH
                     PQVDLATEPRWPRISGTFGEDAQLTARMAKAYILGYQGDKLDSNGVACMTKHFSGGGP
                     QKEGLDPHFPFQKGQVYPGNNFNYHLLPFEAAFAVHTAAIMPYYGVPKGQTSEDVGFS
                     FNKDIITGLLRDKYHYDGVVCTDWGLVTDANMGGMVWPARAWGVEKLSREDRVKKILD
                     AGVDQFGGENCPEIVVQLVKGGRLTEARIDSSARRLLRQKFELGLFDNPFVDETKVGQ
                     VVGNPGSLKAGEASQRRAMTLLKNQDKVLPLAPGKLKIYVRNVDSAVAAQYGTVVDKP
                     EKADIAILRLKTPFVPVETPIMIAKMFHHGDLDFKGKQKDSILQLLKTVPTIVDIYLD
                     RPAVIPEISAKAKGLLADFGASDAAVLDVIFGKDKPGGHLPIELPSSMDAVRAQKEDV
                     PYDSRNPLYKFGFGLSY"
     gene            complement(2493..>2906)
                     /locus_tag="VKQ52_01935"
     CDS             complement(2493..>2906)
                     /locus_tag="VKQ52_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85964.1"
                     /translation="RPLEKTTGELKTKVMGFFGARGGGGASLVVDGPRSPARLKAGSD
                     IRFAIKLGSMMDPSSLIKLYRFDAQKKTREAPANGDSKHLVDCNIKKSGSDVYLLIPA
                     SRLTPGEYGFQNMMMVNGSGMGHMSYTFFAFGVDQ"
BASE COUNT          563 a          696 c          968 g          679 t
ORIGIN      
        1 ccccgggttt tgcgcggagc cgtacggaat aaccgctgtc ctggccaccc gtgaccgaag
       61 ctgcgccctt tgtattgggg atgcggtagg cctgggtctc ctggcccggg gtggtgggga
      121 gcaggtaggt gaagttgggg ttgacgacgg ggttgaggag gcccttgcgg ttgccgcttt
      181 tatagttgat ccttgtatcc tttgtcttgt cgagggaggt gagaacgagg agcctgcttg
      241 tcccgggttt tagctcgaca tcgtagggga ggggatggtc ggagttgagg ttggtcaggg
      301 tggggaagtc gatatgcagg acgtacgtac aataggcctt attgttcgcg gtgaagatgt
      361 agcggccctg gttgtcctgg gtatagctga tatcgatgat cttttgggcg tggaggccta
      421 gctgtgtgag gagggcgata aacaggcatg cctttttcat attgtgggga ttggttttcg
      481 gctaaaggta ggaagttagg ctgaaaatgt ttttggcgaa ttgctattta tgcatacttt
      541 gtgtcatact aacatattgc tgctatgcct gccataaaac ttctttccgc cccggcgttg
      601 ctgatcgcgg cgtttgggtt tgcctcctgt aaccagggcg cttctacagc ttcgggtggc
      661 gccgaatcct ccgtcacgaa gacggcggat ggatatacct accgggatct taacaagaat
      721 ggaaaattgg atgtgtatga ggatggccgc cagcctgtcg atgcgcgggt ggaggacctg
      781 ttgaagcaga tgagtctcga agagaaagcg gggctgctgt tcatcaatgg ggcgcgggtg
      841 aatgacgacg ggtccgtcgg ggataagcct gcgaagggga tgtttgcgtt tgcgcccaat
      901 gccctgaagc tggtgcagca gcaaaagatg aatcatttca atctctgggc cgtgccttcg
      961 ccggcggcgc tggcgaagtg gtacaacagc atgcagcgct acctgcagga ttcgacgcgg
     1021 ctgggtatcc cgatgacgat cgcttcggac ccgcgcaatc acttcgccag taatgtcttt
     1081 gccatgtcgg ccaatacttt ttcgcagtgg tgtgagcctc ttggattagg ggccatcggg
     1141 gattctgttt tgacgcggca gtttgcggat gtctcgcgcc gggaatatct ggccgtcggt
     1201 atccgtgagg ccttgcatcc ccaggtggac ctggcgacgg agccgcgctg gccgcggatc
     1261 agcggaacct tcggcgagga tgcccagctt acggcgcgga tggcgaaggc gtatatcctg
     1321 ggttaccagg gggacaaact ggattccaac ggtgtggcct gtatgacgaa gcatttctcc
     1381 ggcggcggtc cgcagaagga agggctcgat ccccattttc ctttccagaa ggggcaggta
     1441 tatcccggga acaattttaa ttatcacctg ctcccgttcg aggcggcttt tgcggtgcat
     1501 accgcggcca tcatgcccta ctatggtgtt ccgaaagggc agacgagcga ggatgtcggg
     1561 tttagcttca acaaggatat catcaccggg ctgttgcggg ataaatatca ttatgatggc
     1621 gtggtctgta cggactgggg gcttgtcacc gatgccaata tgggcgggat ggtgtggccg
     1681 gcccgggcct ggggtgtgga gaagttgtcg cgggaggaca gggtcaaaaa gatcctcgac
     1741 gcgggtgtgg accagtttgg cggggagaac tgcccggaga tcgtggtgca actggtgaag
     1801 ggtgggcgcc tcaccgaggc gaggatcgat tccagcgcga ggcggctttt gcggcagaag
     1861 ttcgagctgg ggctttttga caatcccttt gtcgatgaga cgaaggtcgg ccaggtggtc
     1921 ggcaaccccg gatcgctcaa ggccggggag gcttcgcagc gccgggcgat gacgctgttg
     1981 aagaaccagg ataaggtgtt gccactggcg ccgggcaaac taaagatcta tgtgcggaat
     2041 gtcgattcgg ccgttgcggc gcaatatggt acggtggtcg acaagcctga gaaggcggat
     2101 attgccattc tgcggttgaa gacgcctttt gtgccggtgg agacaccgat catgatcgcg
     2161 aagatgttcc atcatggcga cctcgatttt aagggaaaac aaaaggatag catcctgcag
     2221 ttgttgaaga cggtaccgac gatcgtcgat atctacctgg accggccggc cgttatcccg
     2281 gagatcagcg cgaaggcgaa gggtttgctc gcggatttcg gggcgagcga tgcggccgtg
     2341 ctggatgtca tttttggcaa ggacaagcct ggcgggcatc tgcctatcga actgccttcc
     2401 tccatggatg cggtgcgggc gcagaaggag gatgtgccat atgacagcag gaacccgtta
     2461 tacaagttcg ggtttggatt aagttattga tcttattgat cgacgccaaa ggcgaagaag
     2521 gtatagctca tatgccccat accggagcca ttgaccatca tcatgttctg aaacccatat
     2581 tctcccggtg tcagccggct tgccgggatc agcaggtaga catcgcttcc ggacttcttg
     2641 atgttgcagt cgaccaggtg tttgctgtcc ccgttggccg gggcttcacg ggtcttcttt
     2701 tgcgcatcga accggtagag cttgatgagc gaggaagggt ccatcatcga gccgagcttt
     2761 atggcaaacc ggatgtcgct gccggctttt aaccgggcgg gcgagcgtgg gccgtctacg
     2821 acgaggctgg cgccgccgcc gccgcgcgca ccgaagaagc ccattacttt cgtttttagc
     2881 tcaccggtag tcttctccag cggcct
//
LOCUS       DATGFG010000103         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_96446, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000103 DATGFG010000000
VERSION     DATGFG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96446"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2760)
                     /locus_tag="VKQ52_01940"
     CDS             complement(<1..>2760)
                     /locus_tag="VKQ52_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ85965.1"
                     /translation="LEVVDHILYTAGGNSLYRYDPAANTYTRVWEFPADGHTPDEIWT
                     LKNDGNSALLMGTINGRVLTWDIKTSRLLQEPLADSLGILQYGTIYDLYVGAAGYKWI
                     ATAKGGIGVIDPQRSRFHTFSHLPGIAGSPADFVTSFVETPDSNLWIGTEGNGILVYN
                     RRTEHYTRFRTRTAGSKQFPGDKVSNMCLDQGAHIWAAEQSAGIDRIDPATGHTDHYY
                     CNRTAAGLESNWVWMIYATRSNDCWATTLRQVSLMGALYRFDRAANRFTVFDTSLSDV
                     FTLYEDRQGTLWGGNLAQLIAIDRVGRHHRYYPIGFSVRCIYEDKAGRLWLGAEGGGL
                     LLFDRQRNTVIARYTTDEGLCNNTVNSILEDSAGSLWLSTANGLSRFNPATTRFRNYF
                     EADGLQSNEFIYNAAAALRSGEFAFGGIKGFNLFDPLTIRETGNMPGLRLTGITVNGT
                     PIEKDPSLVSKWGADQVEELRVPYDKAFLSFDFTALEFSAPQEIDYAYFMDGWDRKWT
                     DAGHQRKAAYTHLGEGHYLFRVRSSNTEGKWNPAEIRLSITVLPPWYRTAWAYAFYAL
                     VIGALLVIYRLYKARQARLKYEIAVAKHNADFFTNISHEFRTPLTLIINPAKDLLKKA
                     AAKDPASDPGAGLEHAELNVIHRNARRMLSLVDQLLLFRKADSGADRLIPTRLNMIDL
                     AREVYLSFAQQARAKDIRYEFNCDRDSIEACADREKVEIILFNLVSNALKYTPPGGAV
                     SLSIAETAGRIEFRVTDSGPGIPAAAGDQIFGRFYQAADTTTRPKPGFGIGLYLARQF
                     AVAHKGELSYTSEVGKGATFTLRLPKGTAQPGDTPPPYTGGQTSQLFQELVTADMPEE
                     SPDPAQQAPETLAPLVDEKRSVLIIDDDQAMRNYIGSIFRESMTIYEAADGAEGLRLT
                     LEHLPD"
BASE COUNT          455 a          705 c          994 g          606 t
ORIGIN      
        1 gtcgggcagg tgttcgaggg tgaggcggag gccttcagcg ccgtcggctg cttcgtagat
       61 ggtcatggat tcccggaaga tggagccgat gtaattgcgc atggcctggt cgtcgtcgat
      121 gatgagcacg gagcgtttct cgtcgacgag gggcgccagg gtttcgggcg cctgttgtgc
      181 gggatcgggg gattcctcgg gcatatcggc ggtgaccagt tcctggaaca gttggctggt
      241 ctggccaccg gtgtagggtg gcggggtatc gccgggctgt gctgttcctt ttggcagccg
      301 aagggtgaag gtcgcgcctt tgcccacttc acttgtgtaa gacagctctc ctttatgggc
      361 aaccgcaaat tgccgggcga ggtagaggcc gatgccgaac cctggttttg gccgggttgt
      421 cgtgtcggcg gcctggtaga agcgcccgaa gatctggtca ccggcggcgg cggggatccc
      481 ggggccgcta tccgtcacgc ggaattcgat ccggccggcg gtttcggcga tggagaggga
      541 gacggcgccg ccggggggtg tatatttcag ggcgttggat acgaggttga agaggatgat
      601 ctctactttt tcccgatcgg cacaggcttc gatggaatcc ctgtcgcagt tgaattcgta
      661 gcggatatcc tttgcacgcg cctgttgcgc gaagctgagg tagacttccc gggcgaggtc
      721 gatcatgttc agccgggtgg ggatcaggcg gtcggcgccg ctgtctgctt tccggaacag
      781 gaggagctgg tcgacgaggc tgagcatccg gcgggcgttg cgatggatga cgttgagctc
      841 cgcgtgttcg aggcctgccc cggggtcgga ggcggggtcc ttagcggcag ccttcttcag
      901 gaggtctttt gccggattaa tgatcagggt aagcggggtg cggaattcgt gagagatatt
      961 cgtgaagaag tccgcgttgt gttttgccac ggcgatctca tatttcaggc gggcctgccg
     1021 ggccttgtag agccggtaga tcaccagcag ggcgccaatg acgagggcgt aaaaggcata
     1081 ggcccaggcg gtgcggtacc agggtggaag aacggtgatg ctcaaccgga tctccgccgg
     1141 attccatttc ccttccgtat tggagcttct tacccggaac aggtagtgac cttccccgag
     1201 atgggtgtag gcggctttgc gctggtggcc ggcatcggtc catttcctat cccacccatc
     1261 catgaagtag gcgtagtcga tctcctgcgg cgcggagaat tcgagcgcgg tgaagtcgaa
     1321 ggagaggaag gctttgtcat aggggacgcg gagttcttcc acctggtcgg cgccccattt
     1381 gctgacgagg gaggggtctt tttcgatggg ggtgccgttg accgttatgc cggtgagccg
     1441 gagacccggc atgttgcccg tttcgcggat ggtaagcggg tcgaagaggt tgaacccttt
     1501 tatcccgccg aaggcgaact ccccggagcg cagggcagcg gcggcgttgt agatgaattc
     1561 attgctttgc aggccgtccg cttcgaagta gttgcgaaac cgggttgtcg ccggattgaa
     1621 tcgggagagg ccgttggcgg tgctcagcca gagggaaccg gcgctatcct ccaggatgga
     1681 gttgacggtg ttgttacaga gcccttcgtc ggtggtatag cgggcgatga cggtgttccg
     1741 ttgccggtcg aagagcagca ggccgccgcc ttcggcgccc agccagagcc tgccggcctt
     1801 atcttcatag atgcagcgca ccgagaaacc gatgggatag tagcgatgat gccgtcctac
     1861 cctatcgatg gcgatgagct gcgccagatt gccgccccat agcgtgccct gtctgtcttc
     1921 atagagggtg aacacgtccg acaggctggt gtcgaatacc gtaaagcggt tggccgcccg
     1981 gtcgaagcgg tagagggcgc ccatgaggct tacctggcgg agggtggtcg cccagcagtc
     2041 gttgctgcga gtagcataga tcatccagac ccagttgctt tccaggccgg cggccgttct
     2101 gttgcaataa tagtgatcgg tgtggccggt ggcggggtcg atccggtcga tgccggctga
     2161 ttgttcagcc gcccagatat gggcgccctg gtcgagacac atgttggaca ctttgtcgcc
     2221 ggggaactgt ttgctgccgg cggtgcgggt ccggaagcgg gtgtaatgtt ctgtccggcg
     2281 gttgtatacc aggatgccgt tgccttcggt gccgatccat aggttactgt cgggtgtttc
     2341 gacgaaggag gtgacgaagt cggcgggact gccggcgatg ccggggaggt gggagaaggt
     2401 atgaaagcgg ctgcgttggg ggtcgatgac gcctataccg ccttttgcgg tggcgatcca
     2461 tttgtatccc gcggcgccga cgtacaaatc gtagatggtg ccatattgca ggatgccgag
     2521 gctatcggcc agcggttcct gcaggaggcg acttgtcttt atgtcccagg tgaggacgcg
     2581 gccgttgatg gtccccatca ggagggcgct gttgccgtca tttttcagcg tccagatctc
     2641 gtccggcgtg tggccgtcgg cggggaattc ccatacccgc gtataggtgt tggccgcggg
     2701 gtcataccgg tagagggagt tgccgccggc ggtgtagagg atgtggtcga ccacctcgag
//
LOCUS       DATGFG010000104         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_910882, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000104 DATGFG010000000
VERSION     DATGFG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910882"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2130
                     /locus_tag="VKQ52_01945"
     CDS             <1..>2130
                     /locus_tag="VKQ52_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85966.1"
                     /translation="SLSSIPVPQGSKSRSGIGRQVTKFYTARDFPVYYSNTGLDPGTN
                     MEAHDASTTNFFYKYAFDARALSQGFLVATNDMHGKILSEASYADNDTTVRVNYKQYY
                     YRNTGVNGLNETFPFVSSKQGGAITQGNMGIDIELMTDTREFIVKSSGLEVQGQADLY
                     PVIFPIWLPFIWPVTANSENDYRAVTTTKVINYHGVLDSIVVFDKGSKVSTRNLLYDG
                     ETGQVIATRTINEFDQPVYSTSYPAWWAYDGMGPAYQNTGLRYSSPINFTDGRLASNN
                     VDVSQLVSGDELLITTPGATPPGCLSIASSEVQIWVMDLNKNNAPSFPAATPNFIFID
                     KSGNPYNNANVTGIRVIRSGRRNMLEDKVATVTSLASPIVTNGSVQQLSMGASSAVLH
                     ATAVEYSEKWQTDKDEIGTFSSVYDQNSCTSSLVQDCNGQPEKTINPYRKGLLGTFRV
                     SRSLVFYGHRNDSLITQNVNLSSDGWLARFGLYWDFNGGGLVPNTSNPLWMESDRVNR
                     VNAQGLQLETRNALGIYTAAQYGFNKTLPLAVTNNSPFSHAAYAGFEDYGFNQGIDGF
                     QPYPCSTEPIDFAHMGNVQVVPTDGMNFRAHTGKYVLQLNQGSVGTVQLPITPGDVLN
                     YNLSFGTTTTQSFVNPGVNSSFSPATGGSLSYSNASTSATGTTVSTGMDAWQSAHTGF
                     SFNWDGYIVINTKGTYHFPQSCSSSYNL"
BASE COUNT          496 a          570 c          601 g          463 t
ORIGIN      
        1 tcgttatcga gcattccagt gccgcaggga tcgaagtccc ggtcgggtat cggcaggcag
       61 gtgacgaaat tctataccgc cagggatttt cccgtctatt acagcaatac gggcctggat
      121 cccggaacca atatggaagc gcatgatgcc tccaccacca atttctttta caagtacgcg
      181 tttgacgccc gggcgctttc ccagggattc ctggtggcga caaacgatat gcacggtaag
      241 atcctgtcgg aggcttccta tgccgacaac gacaccacgg tgcgggtcaa ttataagcag
      301 tactactatc ggaacacggg ggtcaatggg ttgaacgaga ccttcccttt tgtttcgtca
      361 aagcagggtg gggccattac ccaggggaat atgggcatcg atatagagct gatgaccgac
      421 acacgggagt tcatcgtaaa gagcagtgga ctggaagtcc aggggcaggc agacctttat
      481 cccgtgatct ttcctatctg gttgccattc atctggcctg taaccgcgaa cagcgagaac
      541 gattaccggg ccgtcacgac gacgaaggtg atcaactatc atggcgtgtt ggacagtatc
      601 gtggtattcg ataaggggag caaggtatcg accaggaacc tgctgtatga cggcgagaca
      661 gggcaggtta tcgcaacgcg gacgatcaat gagttcgatc agcccgtata ttcgacttct
      721 tatcctgcgt ggtgggctta tgacgggatg gggccggcct atcagaacac gggtctgcgg
      781 tattccagtc caataaattt cacggacgga agattggcca gcaacaatgt cgacgtgtcc
      841 cagctggtga gcggtgatga actgctgatc accacccccg gggcgacgcc gccaggttgt
      901 ctgtcgattg cttcgtcgga agtccagatc tgggtgatgg acctgaacaa gaacaatgcg
      961 ccttctttcc cggcggcaac ccccaatttc atttttatcg acaagtccgg gaatccgtat
     1021 aataacgcca atgtcaccgg tatccgggtc atccgaagcg gccggcggaa tatgctggag
     1081 gacaaggtgg ctacggtgac aagcctggcc agcccgatag tgaccaacgg ctccgtgcaa
     1141 cagctttcga tgggcgcctc cagtgcggtg cttcatgcca cggcggtaga atacagcgag
     1201 aagtggcaga ccgacaagga cgagatcggc acgttcagtt cggtatacga tcagaacagc
     1261 tgtacgagct cactggtgca ggactgcaac gggcaaccgg agaaaaccat caatccttac
     1321 cggaaagggt tgctgggcac attccgcgtc tcccgcagcc tggtatttta tggtcaccgg
     1381 aatgactccc tcatcaccca gaatgtgaac ctatccagtg acggctggct tgccaggttc
     1441 ggcctatatt gggatttcaa cggcggaggc ctggtcccga atacctccaa tcccttatgg
     1501 atggagagtg accgcgtgaa ccgggtgaat gcgcaggggc tacagctgga gacccggaat
     1561 gcactcggga tctatacggc ggcgcaatat ggattcaaca agaccctgcc gttggctgtc
     1621 accaataatt cgccattttc ccatgcggca tatgcgggat tcgaagatta tggcttcaac
     1681 cagggcatcg atggatttca gccctatccc tgttcgaccg agccgatcga ttttgcgcat
     1741 atgggcaatg tgcaggtggt gcctacggat gggatgaact tccgggcgca tacgggtaaa
     1801 tacgtgctgc agctgaacca gggcagtgtc gggaccgttc agctcccgat caccccaggc
     1861 gatgtgttga actacaattt atccttcgga accacaacga cgcagtcatt tgtcaacccg
     1921 ggggtcaatt cctcgttttc acccgccacc ggcggcagcc tcagctactc aaacgccagt
     1981 acttccgcca ccggcactac ggtgagtacg ggcatggatg catggcaaag cgcgcacacg
     2041 ggattctctt tcaattggga tggttatatc gttatcaaca cgaaggggac ctaccatttt
     2101 ccccagtcgt gtagttcaag ctacaacctt
//
LOCUS       DATGFG010000105         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_554183, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000105 DATGFG010000000
VERSION     DATGFG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554183"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VKQ52_01950"
     CDS             complement(<1..324)
                     /locus_tag="VKQ52_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ85967.1"
                     /translation="MRKNQLQMALSILSILFSLTVFSQTPLTLTGTVRQSVTKDRVPA
                     VSVTVKGTSIGTFTDDRGNFKLAITQQPPYILVFSSIGYETQEVSVSSAGTPVTVDFV
                     PSSTLG"
     gene            474..1400
                     /locus_tag="VKQ52_01955"
     CDS             474..1400
                     /locus_tag="VKQ52_01955"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF009251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HLZ85968.1"
                     /translation="MKQLQFPHQTNFYRGKVRDVYTISDKWLVMIASDRISAFDVILP
                     RPIPYKGQVLNQIAAYMLKATSDICPNWLADTPAPNVAIGHKCTPFRVEMVVRGNLTG
                     HAARTYAAGKRELCGVHLPEGMKENDYFPTPIITPSTKAEVGHDEDISREEIIRRGIV
                     NETDCAQLEKYTLALFARGRELAAKRGLILVDTKYEFGTIGDTVYLMDEIHTPDSSRY
                     FYAKGFEERQAKGEKQQQLSKEFVREWLIANNFMGKEGQTVPVMTDEWVDTISKRYIE
                     LYEQVIGEPFRPQPLSDEETYQRVLYSLGNLG"
     gene            complement(1555..2994)
                     /locus_tag="VKQ52_01960"
     CDS             complement(1555..2994)
                     /locus_tag="VKQ52_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ85969.1"
                     /translation="MNKAAKSFVRFRGYVWNIGYRASLGSYERRKLGIFNFMNFLALS
                     AGIVVPIIGLFNKGHFPSTIWLVAGAPALISACVLLANHYQRYEFARLLYFTLYPLVT
                     TLSYALRIDAGIGFFFVLYGVLSVFFLQSIYNIVFSFTLSMACYFLVAVIWKDYDYSL
                     EKHNFYFYIFNHLLAVFFIFYGLLLIKNENTRYQNQTRDKNWQLRRSNLKILRQKADI
                     SGKAALLEQQTHQLTELNSLKNKLFSIIAHDLKAPLYALRNLFRNVQLYDLPGDEIKI
                     LIPEVVNELTYTTGLMENLLQWAKSQMQAESVRPQLLDIARITKEVLQLLRLQAEAKH
                     IYINSKIERPVYVYADRDMINLVLRNLLSNAIKFTPEEGSIWIEAREMRSHIEVLVQD
                     TGMGIPPEGLRKLMDAHYYSTRGTVGEAGTGLGLMLCKEFLSRNGGQMRIESEPGKGS
                     TVSFTLPKGGAAPNESGAGDVAPKDRADA"
BASE COUNT          871 a         1055 c          804 g          676 t
ORIGIN      
        1 cccaagcgtg gaagaaggca caaaatccac cgtcaccggg gtgcccgcac tggagacact
       61 cacctcctgc gtttcatagc cgatcgacga aaagacaagg atatacggcg gctgctgcgt
      121 aatggccagc ttgaaattcc cccggtcgtc cgtgaacgtg ccgatggaag tacccttgac
      181 ggtcacactc acagccggca cacggtcctt cgtcaccgat tgccggacag taccggtaag
      241 cgtaagcggg gtttgtgaaa agacggttag ggaaaaaagg atagaaagga tggacaaggc
      301 catctgcagt tggttctttc tcattgcgca agttttcgtt taaaatgacc ttcggccagc
      361 ggacctacgg ttcgttggta agcgatgtgt attactaaaa tacacattgt aatgttgacc
      421 tgaaaattta attttgcgcg ctaaggggac agaccgcccc gcaatcgatc aatatgaagc
      481 aacttcaatt cccccatcag acgaatttct atcgcggaaa ggtgcgcgat gtctatacca
      541 tctccgacaa atggctggtt atgatcgcat ccgatcgcat ctcggcattc gatgtcatcc
      601 tgccgcgccc catcccctac aaaggccagg tgctcaacca gatcgccgcc tatatgctga
      661 aggccaccag cgacatctgc cccaactggc tggcggatac ccccgccccg aatgtggcca
      721 tcggccacaa atgcacaccg tttcgggtag agatggtcgt caggggtaac cttaccggtc
      781 atgcggcacg cacctacgcc gccggaaagc gcgaactttg cggcgtacac ctccccgaag
      841 gaatgaaaga aaatgactac ttccccaccc ccatcatcac cccctccaca aaggccgagg
      901 tcggccacga tgaggacatc agccgggaag agatcatccg ccgcggcatc gtcaacgaga
      961 ccgattgcgc ccaactggaa aaatacacgc ttgccctctt cgcccgcggc cgggaactcg
     1021 cggcaaaacg cggcctcatc ctcgtggaca cgaaatatga gttcggaacc atcggcgaca
     1081 ccgtctacct catggacgag atccacaccc ccgactcgtc acggtatttc tatgcaaaag
     1141 gcttcgaaga gcggcaggca aaaggagaga aacaacaaca gctgagcaag gaattcgtga
     1201 gggaatggct catcgccaac aattttatgg gaaaagaagg ccagacggta ccggtcatga
     1261 cggacgaatg ggtcgacacc atcagcaagc gctatatcga actatacgaa caggtcatcg
     1321 gcgaaccctt ccggccccaa ccgctcagcg acgaggagac ctaccagcgc gtcctctact
     1381 ccctgggcaa cctgggctaa cgcgcctccg gcgccatatc ccgccgcccc aatacttcct
     1441 atccacgggt gccggccaat catcgactgg caccccaccg gcatcatcgg ttcatcggct
     1501 cgccgccgcc ccctacgcat cccccgggtc ctccggcacc atatcccgcc gcccctatgc
     1561 atcggcccgg tccttcggcg ccacatcccc cgccccactc tcattcggcg cggcaccgcc
     1621 cttcggcagg gtgaacgaga ccgtactgcc cttccccggc tcgctctcga tccgcatctg
     1681 cccgccatta cgggaaagga actccttgca aagcatcagc cccaaccccg tcccggcctc
     1741 gccgaccgtc ccccgggtgc tgtaataatg ggcatccatc agcttccgta aaccttcagg
     1801 ggggataccc atacccgtat cctgcaccag cacctcgata tgcgaccgca tctcccgcgc
     1861 ctcgatccag atggagccct cctcgggcgt gaacttgatc gcattggaaa gaagattccg
     1921 caacaccagg ttgatcatgt ccctgtcggc atacacatac accggccgct cgatcttgct
     1981 gttgatataa atatgcttgg cctccgcctg caaccgcagc agctgcaaca cctccttcgt
     2041 gatccgcgca atatccagca gctgtggccg caccgactcc gcctgcatct ggctcttcgc
     2101 ccactgcaac aggttctcca taagccccgt cgtataggta agctcgttga ccacttccgg
     2161 gatcaggatc ttgatctcat cccctggcag atcatacaat tgcacattgc ggaacaggtt
     2221 ccgcaacgca tacagcgggg ctttaaggtc atgcgcgatg atggaaaaaa gcttgttctt
     2281 aagagaattc aactccgtca gctgatgggt ctgctgctcc agcaacgccg ccttacccga
     2341 gatatccgcc ttctgccgca ggatcttcag attgctccgc cgcaattgcc agttcttgtc
     2401 ccgcgtctgg ttctggtaac gcgtattctc attcttgatc agcaacaaac cgtagaaaat
     2461 aaagaatacg gcaagcaggt gattgaaaat atagaaatag aaattatgct tctccaggct
     2521 gtaatcgtaa tccttccaga tcaccgccac aagaaaataa caggccatcg aaagcgtaaa
     2581 ggaaaaaacg atattgtaga tgctctgcag gaagaacacc gaaagcacgc cataaaggac
     2641 aaagaagaac ccgatccccg catcgatccg caacgcataa ctcagggtcg tgaccagcgg
     2701 ataaagcgta aaatacaaaa gccgggcaaa ctcgtatcgc tggtagtggt tggccagtag
     2761 cacacaggca ctgatcagcg cgggagcgcc ggcgacaagc cagatagtgg acgggaaatg
     2821 acccttgttg aaaagaccga tgatggggac tacgataccg gcagacagcg ccagaaaatt
     2881 catgaagttg aaaatgccca gtttccgtcg ctcatagctc ccaagcgaag cccggtagcc
     2941 aatgttccag acatatcccc ggaaccggac gaaacttttt gctgccttat tcatggtcag
     3001 agccttggag tttagtttta agggttacaa accaggggac gggcattgac ggtttagcta
     3061 ctaagaaaaa gattataaac gtaaaaaata cacgttttgt tggatcaaat ctatctcatt
     3121 ttggctgaaa caaaaactac tatacattct tcctttttct acatcatctc ccccgtccag
     3181 gacaaacccc taccccttcc gtctttttta tccccggaaa cgtccttcca tggccgcttt
     3241 taaccgaaaa ttggtccaaa taggacgcat ccggcacaaa aacacagtag aaaaaactcc
     3301 tatatcatcg ggccaaaaaa cgacaccggg ttaaaaaaga ccgggccgga gtgctcatcc
     3361 cctatccctc accgccaccc gccaccttcg catctccctc ggccgc
//
LOCUS       DATGFG010000106         6709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_438112, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000106 DATGFG010000000
VERSION     DATGFG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438112"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1291)
                     /locus_tag="VKQ52_01965"
     CDS             complement(44..1291)
                     /locus_tag="VKQ52_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85970.1"
                     /translation="MKKGFLPLMVLLFVYADIVSGQTTRQPALIVMNNGDTLHGWIDY
                     KGWERNPGVISFRYDSLSSNSTTYGVADLRYFEIASVEAYERATIKQEVPGLATPQGL
                     VGQGAGADSLIAKTVFLRLLVKGNRISLYKWNENKECFYIRETGGDLEELVLQINSND
                     NTIAPIPLYKGTLKIHAVKYDLASLSPAIDNAGYDEHDLTKIVTSINGASNSVLYTTY
                     VIHNKVHWFVGGGAGVSGLTISGDKSYLGQIAFKKTVVAPYVLLGFDYLFSNGVGPWG
                     MRLELAYSSASYKATGTATETSLDHVTYIFQQNNFAPSAYVLYHFFNQPRLKIYCAVG
                     VGANFASYPKNTYQETFPESRTIDNYADLSSFWLSGNLKAGVIFNRKIEAGIVGVYGN
                     YSKEVVYSFDPHTYLFWVGYKFN"
     gene            1510..2088
                     /locus_tag="VKQ52_01970"
     CDS             1510..2088
                     /locus_tag="VKQ52_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HLZ85971.1"
                     /translation="MGKATPSAKPDLRYWWVFLLTGILLILVGCLAFYHPVVTGIVIG
                     LSLGIVVILLGIAHIAFALSNRHLPHWSWHLVMGFIDLLFGAVLLPYPDITVVVLPFL
                     VGFWFLFRGISVVLYAFTLKHFATTGWGWFLGGGIFIAAFALFIIYFPSLGYLSIVAW
                     TAIALIVTGISNILLALRLKNRKQDSEQTLIM"
     gene            2140..2982
                     /locus_tag="VKQ52_01975"
     CDS             2140..2982
                     /locus_tag="VKQ52_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLZ85972.1"
                     /translation="MRTLLILTDFSESSFRAAEYGCSLAGILHIQRIILYHSYSTVIP
                     TTDIPVSDVKTNQQIYLESMQALALLQDRLRTQVDPAVTIELLSEDVFLPEGIDRLCR
                     KKAVDLVVLGASGSSGFERAVMGSTTARLLADPCVPLLIVPAETPIGRGIKTIAFAAD
                     LRNIPHLPVVQLYLFLDALRAEICVVNVEPSADEGKYFPEKKQALLDLHHLLDKYHPS
                     FYDVEGEDVADSILAFADGHKASLIIVVPGTHHYLSSIFHKTVSKRLAYRSTVPLLAL
                     SAAK"
     gene            complement(3027..3950)
                     /locus_tag="VKQ52_01980"
     CDS             complement(3027..3950)
                     /locus_tag="VKQ52_01980"
                     /inference="COORDINATES: protein motif:HMM:NF039394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HLZ85973.1"
                     /translation="MKRSLFLAGMLLPALTYAQNTDSSVRGKFITTAPRLGGITVTDL
                     VTPVKANGNVFTMQAPVVDIGIPLYKDLSPEHATVVKTGIRYEGLFLSNEKNIGGSSF
                     HSLTVPLLYSYSLTRTTSLSLIGLATVASDFKRTIEGEDIQYMAGVRVGFRPGKTLRW
                     GITLTYVSNYSGRFLLPIPDIDWTISPKWSLTGVVPARASLKYALSRTQSLGATVGFT
                     GSMYRLNDGERGQYLHFRQNSGGLLYERRLGQRWKFTLLAGHTFMQRLGTFNIGQKVS
                     FDRFDKLNDRVPNVSYRQNSFVFQGGLSWQF"
     gene            complement(3964..4419)
                     /locus_tag="VKQ52_01985"
     CDS             complement(3964..4419)
                     /locus_tag="VKQ52_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLZ85974.1"
                     /translation="MSSNNAPLFRPLMIFQIGSLVQKMRRECDIIFREKEFPLEMDQI
                     PVLLALYYRFPDGASQQEIGEQLQRDKASINRTITFLVRNDIVNVVQDDTNKRRNRVG
                     LTATGKKLAMQAENVLEKFDSFLSATFTAEEKVRLDDLLQKLIATVNSL"
     gene            complement(4673..5092)
                     /locus_tag="VKQ52_01990"
     CDS             complement(4673..5092)
                     /locus_tag="VKQ52_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ85975.1"
                     /translation="MTNKITPYLLLADDDPDDQELLAHSFLERNPTAEIRYFSDGRDV
                     LTFLNHCPTNELPVVMLLDYKMPIVTGGEVLAAIKDNPRLKEIPKFVWSTSNNTQYSE
                     ECLKRGALEYFVKPSDVAHMEEIVACLTRIFRLRSSA"
     gene            5304..6392
                     /locus_tag="VKQ52_01995"
     CDS             5304..6392
                     /locus_tag="VKQ52_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLZ85976.1"
                     /translation="MDHSAQDNYYHKMIEEVIDYAILMLDANGIIQNWNRGAQRIKGY
                     TEQEIVGRHFHIFYPPEDQAAGLPERLLAEARLNGRAMHEGWRVRKDGTRFWGSIVIT
                     ALHDESGAILGFSKVTRDLTERKAAEDKLLQNARQLEVQNRELQQFAYAAAHDMKEPL
                     RKILFYYSAILAGGETLDADKQRAYLERSADAARRMQGLIDDLLTFTKVVDVVDSFQP
                     VDLNGIAAEVLQFYQDTIDSISATVVVGDLPVLNGIPFQLRQLFLNLIGNSLKYRDSI
                     RPLKIEISSTPPNGFRHISVKDNGIGFHQAHAGKIFQMFERLHGREKYAGTGIGLSIC
                     RKIMDIHNGQIMAHGQPGEGAVFDLYFP"
     gene            complement(6380..>6709)
                     /locus_tag="VKQ52_02000"
     CDS             complement(6380..>6709)
                     /locus_tag="VKQ52_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ85977.1"
                     /translation="FLREVLAHIEENMGNPDLSVGELCQRFHASRSTFYKRLLLLTGK
                     TPVEFIRHIRLKRAAELLEKSQLTIAEIAYTVGFNNPKYFTQYFKEEFGCIPTAWRRD
                     RLFPYGK"
BASE COUNT         1613 a         1929 c         1605 g         1562 t
ORIGIN      
        1 gaattcagct atcgattttt tcatcacacc gacgaaggcc aagtcagtta aacttatacc
       61 ccacccaaaa caaatacgtg tgcggatcaa aactataaac tacctccttg gaatagttcc
      121 cataaacccc gactatgccc gcttcaatct tcctgttaaa gataactcct gcctttaaat
      181 tccctgatag ccaaaaactg ctcagatcag cgtaattatc tatcgtccga ctctcgggga
      241 aagtctcctg ataggtattt ttggggtaag aggcaaaatt agcacctaca cccaccgcac
      301 aataaatttt tagtctcggc tggttaaaga aatgatacaa cacatatgcg ctgggtgcga
      361 aattattttg ttggaagatg taggtcacgt ggtcaaggct ggtttcagtg gcagtaccag
      421 tagccttata agatgccgag gagtaggcca gttccagccg catgccccag gggcccaccc
      481 cattcgaaaa aagatagtcg aatcccagca acacgtaagg tgctaccacg gtttttttaa
      541 aggcgatctg acccaggtaa gacttgtctc cggaaatagt caaccccgaa actccggcac
      601 cgcctcctac gaaccagtga accttgttgt ggataacgta tgtcgtataa agaacgctgt
      661 tcgatgcacc gtttatgctg gttacgatct ttgtgagatc atgttcatca tatccggcgt
      721 tgtcgatagc aggcgacaag gaggcaagat catatttgac ggcatggatt ttcagtgtac
      781 ccttatacaa aggaatgggc gcaatagtgt tgtcatttga gttgatctgc aggaccaatt
      841 cttccaaatc accgcccgtt tcccggatat aaaagcactc tttattctca ttccacttat
      901 aaaggcttat tcggttgcct ttgaccaaaa ggcgcaaaaa tacggttttt gcaatgaggc
      961 tatcggctcc cgcaccctga ccaacgagtc cctgaggagt cgccaaaccg ggcacctcct
     1021 gctttatggt cgcccgctcg tatgcttcaa ccgaagcgat ctcaaaataa cgcagatctg
     1081 ccacgccgta agtggtggag ttggaggaaa gcgaatcgta acggaacgag atgacgcctg
     1141 gattgcgctc ccatccctta taatcgatcc acccatgcag ggtatctcca ttattcatta
     1201 cgataagcgc cggttgacgg gtcgtttggc ctgaaacaat gtcagcgtaa acaaaaagca
     1261 ataccatcag cggtaaaaat ccttttttca tggtctatga tttatggcga ttttggggat
     1321 gctcagtact ataccggcga aaatggcgtg ggaaccgcaa gggaattgct caaataccta
     1381 ctaaacaatg gtcggattaa ccaatgcgaa ccctgattct ttaaggaagt ttaaaccccg
     1441 aatatagcta aaactgccaa ctggtgcata tggcacaggc tttgccgcat gtatgataaa
     1501 ttaaccccta tgggcaaggc tactccatcg gccaaaccgg acctgaggta ctggtgggtg
     1561 ttcctcctga cgggcatcct gctcatactg gtgggatgcc tcgccttcta ccaccctgtc
     1621 gtcaccggaa tcgtcatagg cctgtccctg ggtatcgtcg tcattctttt aggcatcgcc
     1681 cacatcgcgt ttgccctgag caaccggcac ctgccgcact ggagctggca cctcgttatg
     1741 ggatttatcg atctgctctt cggcgccgtc ctgcttccct atccggacat tacagtggtc
     1801 gtcctgccct tcctcgtcgg cttttggttc ctgttccgcg ggatctccgt ggttctctac
     1861 gccttcacct taaagcattt tgccaccacg ggctggggct ggttcctggg cggcggcatc
     1921 ttcatcgcgg cctttgcctt gttcatcatc tattttccct cgcttggcta tctcagcatc
     1981 gtggcgtgga ccgcgatagc cctcatcgtc accggcatct ccaatatcct gctggccttg
     2041 cggttgaaaa atagaaaaca ggatagcgaa cagaccctta ttatgtaagc ctgccgatag
     2101 gcggcagctc ctggcacaat tatttcacaa cgataacata tgagaaccct gctgatctta
     2161 acggactttt ccgaatcctc cttccgggcc gccgagtacg gctgctccct ggccggcatc
     2221 ctgcatattc aaaggatcat cctctatcat tcctattcca ccgtcatccc aaccacggac
     2281 atcccggtgt ccgacgtaaa aacaaaccaa cagatctatc tcgaaagcat gcaggccctg
     2341 gcgctcctgc aggaccgcct gagaacccag gtcgaccctg ccgtcaccat cgaactcctg
     2401 tccgaggacg tcttcttacc cgaagggatc gaccggctat gcaggaaaaa agcggtcgac
     2461 ctggtcgtcc tgggcgcctc cggcagctcc gggttcgaaa gggcggtaat gggcagcacc
     2521 acggcccggc tcctggctga cccctgtgta cccctgttga tcgtaccggc ggaaacaccc
     2581 atcgggcgcg ggataaagac catcgctttc gccgccgatc tcaggaatat cccccacctg
     2641 cccgttgtcc agttatacct gttcctggac gcgctcaggg cggagatctg cgtagtgaac
     2701 gtagaacctt cggcggacga agggaagtat tttcccgaaa agaaacaggc cctgctcgat
     2761 ctgcatcacc tgctggataa gtatcatccc tctttctatg acgtggaagg cgaggacgtc
     2821 gccgatagca tcctcgcctt tgccgacggc cacaaagcat cgctgatcat cgtcgttccc
     2881 ggtacacacc actacctgtc ctcgatcttt cataaaacgg tctccaaacg cctcgcctac
     2941 cgttcgaccg ttcccctgct ggcgctttcc gccgcgaaat gaagcgctcc cgccgggaaa
     3001 caaagcgctc tccgccgcga aatagctcaa aactgccagc tgagcccacc ctggaacaca
     3061 aacgagttct gccggtaaga gacgttggga acccggtcat tcagcttgtc gaaccggtcg
     3121 aacgaaactt tctggccgat attgaaggtc cccagccgtt gcataaaggt atgcccggcc
     3181 aacagcgtaa atttccagcg ctgccccagc ctgcgttcat acagcagccc accgctgttt
     3241 tgccgaaaat gcaggtattg accccgctca ccgtcattca accggtacat gcttccggtg
     3301 aaccctacag tggcccccag ggattgcgtc ctcgataaag catacttcaa cgacgccctc
     3361 gccggcacaa cgcccgtcag gctccacttt gggctgatcg tccagtcgat atcgggaatc
     3421 ggcagcagga acctgccgga gtaattactg acatacgtaa gcgtaatacc ccaccggagc
     3481 gtcttgccgg gacgaaaccc cacccgcacc ccggccatat attggatatc ctccccttcg
     3541 atggtgcgct tgaagtcgga ggcgacggtt gccagcccga tcagcgacag agaagtcgtc
     3601 cgcgtaaggg aataactata gagcaacggg accgtgaggg aatgaaaaga ggagccgccg
     3661 atattttttt cattggaaag aaataatcct tcataccgga tacccgtctt taccaccgtc
     3721 gcatgctccg gcgaaagatc cttatacagg gggatcccga tgtctaccac cggcgcctgc
     3781 atcgtaaaca cattcccgtt tgcctttaca ggagtgacaa gatcggtgac ggtaatccca
     3841 cccagccttg gagcggtggt gataaatttt cctcggacac tgctgtcggt gttttgcgca
     3901 taggtgaggg caggcaatag catgcccgcc aaaaaaagac tccttttcat ttcggggatt
     3961 tgtttacaaa gaattgactg ttgcgattag tttttgcaat agatcatcaa gccgcacctt
     4021 ctcttcggcc gtaaaggtcg cagagaggaa cgaatcgaat ttttcaagca cgttttcggc
     4081 ctgcatcgct agttttttcc cggtcgcggt caatccgacc cggtttcttc gtttattggt
     4141 atcgtcctgg acgacgttga caatatcgtt cctgaccaaa aaagtaatgg tcctgttgat
     4201 ggacgccttg tcccgttgca gctgctcccc gatctcctgc tgcgaggccc catccggaaa
     4261 gcggtaatac agggcgagaa gcaccggtat ctggtccatc tccaggggaa attccttctc
     4321 ccggaagatg atatcacatt cccgccgcat cttctggacc agcgaaccga tctggaagat
     4381 catcagggga cggaacagcg gcgcattatt cgaactcatc cggcaaagat actgcttttg
     4441 ttgatatatc aaccatttga gcagaaaaaa tccattttta ccccaaaaat cctcatcgcc
     4501 tgtgaaggat aaaacgccaa aacaacattc caaggataaa ccccaatata agcgcgacaa
     4561 aaactccttt aacagtatac ttcgatccca aatttacaat tatgataacc cgttagtatt
     4621 aaataattat gccaaattcc gcacccgaat tatgccaaat tccgcacccg aattacgccg
     4681 aactccgcaa ccggaagatc cgcgtaagac aggcgacgat ctcttccata tgggccacat
     4741 cgctgggctt gacaaagtat tccagggcgc cccgcttgag acattcctcg ctgtactggg
     4801 tgttattgga agtactccag acaaacttgg ggatctcttt gagccgggga ttgtccttga
     4861 tcgccgccag cacctccccg ccggtgacga tcggcatttt ataatccagc aacatgacaa
     4921 caggcaattc gttcgtcgga caatggttca ggaaggtcag tacatcccgc ccatcactga
     4981 aataccggat ctcggcagtc gggttgcgct caagaaagga gtgggccaat aattcctggt
     5041 catccgggtc atcatcggcc agtaggaggt atggggttat tttattggtc atgacgggtg
     5101 ttcctcgcgg gtcgaactgc taagatacca aataaacatg cccggattcg atccttcttt
     5161 gtttagcttt tttgtctggt tttgggtaaa aatttgcaca gtttgcggta cgatccggcc
     5221 aaaatccagc cgtcctgtct cctcctgtca ccccccgtcc ggctttttaa ctggcacggt
     5281 tttcctgcca ttttcccttg actatggatc attctgccca ggacaactat tatcacaaga
     5341 tgatcgaaga ggtgattgac tatgccatcc tcatgttgga tgccaacggc attattcaaa
     5401 actggaatcg cggcgcccaa cggatcaaag gatacaccga acaagaaatc gtcggccggc
     5461 attttcatat cttctatccg ccggaagacc aggcagcagg tttgcccgag cgcctgctgg
     5521 ctgaagccag gctcaacggc cgggccatgc acgagggctg gcgtgtccgg aaggatggaa
     5581 ccaggttctg gggaagcatc gtcattacag cgctccacga cgagtccggc gccatcctcg
     5641 ggttctcaaa ggtgacaagg gatctcaccg aacgaaaggc cgcggaagac aaattattgc
     5701 agaatgcccg gcaactggag gtccagaacc gcgagctcca gcaattcgcc tacgccgccg
     5761 cccacgatat gaaagaaccg ctgcgtaaga tcctcttcta ttactcggcc atcctggcgg
     5821 gcggggaaac cctggatgcg gacaaacaac gcgcatatct ggaacggtcg gccgatgccg
     5881 cccggcggat gcaggggctc atcgatgacc tgctcacctt taccaaagtg gtggatgtcg
     5941 tggatagctt ccagcctgtt gacctcaatg gcattgccgc cgaggtcctg caattctacc
     6001 aggataccat cgatagtatc agcgccacgg tcgtcgtagg cgatcttccc gtcctgaatg
     6061 gcatcccctt ccagctacgg cagctgttcc tcaacctgat cggtaattca ctaaaatacc
     6121 gggatagcat ccggccatta aagatcgaga tctcctccac ccccccaaat ggcttccgtc
     6181 atatttcggt taaggacaac ggcatcggct tccaccaggc ccacgcggga aagatcttcc
     6241 agatgttcga gcgcctgcac ggccgcgaaa aatatgccgg caccggcatc ggcttatcga
     6301 tctgccgcaa aataatggat atccataacg gccagatcat ggctcatggc cagccgggcg
     6361 aaggcgccgt cttcgatctc tattttccat agggaaacaa ccggtcccgc cgccaggcgg
     6421 tgggaatgca cccaaactcc tctttaaaat actgcgtaaa atatttcgga ttattgaacc
     6481 ccaccgtata cgcgatctcc gcgatcgtga gctggctctt ctccagcaac tccgcggccc
     6541 gcttcagccg gatatgccgg ataaactcca ccggtgtctt ccccgtcaat aacagcagcc
     6601 gcttgtagaa ggtactccgg ctcgcatgga atcgctgaca aagctcgccg accgacagat
     6661 ccgggtttcc catattctcc tcgatatgcg caagcacctc ccgcaggaa
//
LOCUS       DATGFG010000107         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_437420, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000107 DATGFG010000000
VERSION     DATGFG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437420"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VKQ52_02005"
     CDS             <1..690
                     /locus_tag="VKQ52_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85978.1"
                     /translation="SIFFLPDSYPADVNFSLKPGPIIRNFIAVFTVPQFLIYVFVQAL
                     AFAGLFAYVSGSPVVFMDIFHVDKKIYGWIFAFLSVAFIGLSQFNSLLLRRYTSQQII
                     RVALTAQVVVAGVFWGCAMAGLLSLGVTIGFLFLFLTCLGFTNPNAAALAMAPFAKNA
                     GTASSLLGALQLGIGALASAGVSAFADGTVRPMVAVIAVTSVMALGILVLGRKQPVVV
                     EAGDLGPASGH"
     gene            complement(717..1220)
                     /locus_tag="VKQ52_02010"
     CDS             complement(717..1220)
                     /locus_tag="VKQ52_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85979.1"
                     /translation="MTRIVTLLLIVLVFSAAPISAQQIRFKTYTDSQHKFSIDIPDSW
                     QIKYNKRKGGYIAVPTASEEKVSIDSCHGGIIFRLRFYKPGLKHLHGEKIRGLNWTGI
                     YHSDACGYTCKGDILYGAKGEYQYRGACQYLYFWNNDLTVCIRSNAISFEEAVRKRMI
                     ASFRFFK"
     gene            complement(1282..2001)
                     /locus_tag="VKQ52_02015"
     CDS             complement(1282..2001)
                     /locus_tag="VKQ52_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ85980.1"
                     /translation="MKTVLILGANSDTAKEALALFVEKGYQVIAASRSLKEIDAFIVD
                     RHLPEDKVITRFFDATAFCDHAAFYAGLPAKPNIVLYAAGFQVTNDEALLSWEGSYRM
                     MNVLYAGAVSILNIIVTDGANKNLERIVGLSSLSGVRGRKSNFIYGSTKAAFTTYLAG
                     LRQYLAPRKVSVNAIIAGYINSKLTAHLDLPKNLLMEPAFIARHIVGAGNSFTIVPGW
                     KWKIIYRILKLLPENLVAKLP"
     gene            complement(2116..2802)
                     /gene="rpiA"
                     /locus_tag="VKQ52_02020"
     CDS             complement(2116..2802)
                     /gene="rpiA"
                     /locus_tag="VKQ52_02020"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HLZ85981.1"
                     /translation="MPDYKQQAAQEAAKLIQPGHAIGLGAGSTMTHLITAIKDTPGLV
                     QTLTVASSSFATRQTLLQQGFNVKDSGWLSRLDYYFDGCDQFDRRLTALKSGGGVHTA
                     EKVLAAMADTFVLVGDAAKRVDRLSNTYPLVVEVIPEALAYVSARLKQYFNPTRSELR
                     LSDKKDGAVITERGNFLIDNWFDSFPEPGILNDRVTTIPGILEHSLFYNMAHKAITAG
                     PDGMMVYTKN"
     gene            complement(2994..>3347)
                     /locus_tag="VKQ52_02025"
     CDS             complement(2994..>3347)
                     /locus_tag="VKQ52_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HLZ85982.1"
                     /translation="PTDLHNYHFTIKYDRPVVITEFGGDALAGFHADPDTRWSEEYQE
                     ALYTNQLTMLSGITALRGITPWILADFRSPRRQHPIYQNFWNRKGLISEQGVKKRAFF
                     TLKNFYDTIAKKYAE"
BASE COUNT          592 a          719 c         1147 g          889 t
ORIGIN      
        1 agcatctttt ttcttcccga tagttacccg gcggatgtca atttcagctt gaagccgggg
       61 ccgatcatcc ggaattttat tgccgttttt accgttccgc aattcctgat ctatgttttc
      121 gttcaggcgc tggcgtttgc gggtttgttt gcctatgtgt cggggtcgcc ggtggtcttt
      181 atggatatct tccatgtcga taagaagatt tatggatgga tctttgcctt tttatcggtg
      241 gcgttcatcg ggctgagcca gttcaatagt ctgctgcttc gccgttatac gagccagcag
      301 atcatccggg tggccctgac ggcgcaggta gtggtggcgg gggtattctg gggttgtgcg
      361 atggccgggc tgcttagcct gggggtaacg atcgggttcc tttttctttt cctcacctgt
      421 cttggtttta ccaatccgaa tgcggccgcg ctggcgatgg cgccgtttgc gaagaatgcg
      481 gggacggctt cgtcgttatt gggcgccctg caattgggga tcggggccct ggcctccgcg
      541 ggggtaagtg cgtttgccga tgggacggtg cggccgatgg tggcggttat tgcggtgact
      601 tcggtgatgg cgctggggat attggtgttg gggcggaagc agccggtggt ggtggaggca
      661 ggggacctag gcccggcgag tgggcattag gcggcgttgt tcgtgagggc tatcggtcat
      721 ttaaagaaac gaaagctggc gatcattctt ttgcggacgg cttcttcgaa ggatatggcg
      781 tttgaccgga tacaaacggt tagatcatta ttccagaaat agagatactg gcaagcacct
      841 ctatattgat attccccttt tgcaccatac aaaatatcgc ctttgcacgt atatccgcaa
      901 gcatcggaat ggtagatacc cgtccagtta agtcctctta ttttttctcc atgcagatgt
      961 tttaacccag gcttatagaa cctcagccgg aatatgatgc ctccatgaca ggagtcgatc
     1021 gatacttttt cctccgatgc ggtagggacg gcaatataac cgccctttct tttgttatac
     1081 tttatctgcc aggagtcggg gatatcgatc gagaatttgt gttggctatc ggtataggtt
     1141 ttaaaccgga tctgctgggc ggagattggc gctgcagaga aaacgaggac gatcaggagc
     1201 aatgtgacga tgcgggtcat agtcaggggg ttagtgtgag gcgtggattc cgatgggcgt
     1261 tgagctgcga gctgtggccg gttacgggag tttcgccacg aggttttcgg gaaggagttt
     1321 caggatgcgg taaattatct tccatttcca gccggggacg atggtgaagc tgttgccggc
     1381 gccgacgata tggcgggcga taaaggcggg ttccatgagg aggtttttgg gcaggtcgag
     1441 atgggcagtg agcttgctgt tgatatagcc ggcgatgatg gcgttgacgg agaccttgcg
     1501 gggcgcgaga tattgacgga ggccggcgag ataagtagtg aaggccgcct ttgtgctgcc
     1561 ataaatgaaa ttgcttttgc ggccgcggac gcccgaaagg gaagagaggc cgacgatccg
     1621 ttcgaggttt ttgttggcgc cgtcagtgac gatgatattg aggatggaga ctgcgccggc
     1681 atagagtacg ttcatcatgc gatagctgcc ctcccaacta aggagggctt cgtcatttgt
     1741 aacctggaag ccggcggcgt agaggacgat attgggtttt gcgggcaggc cggcatagaa
     1801 agcagcgtga tcgcagaagg ccgtggcgtc gaagaagcgg gtgatgacct tgtcttcagg
     1861 aagatggcgg tccacgatga aggcgtcgat ctctttcagg ctgcgggagg cggcgatgac
     1921 ctggtagccc ttttccacaa agagggcgag ggcttctttg gctgtgtcgg agttggcgcc
     1981 gaggatgagg acggttttca tgcgcaatgt ttttggcttt cgggaggttt ttttgctttg
     2041 ggagatgtct tttgctttcg gagatttttt gctttgggga gatgtctttt gctttcggga
     2101 gatgtttttc gggctttagt tttttgtgta taccatcatt ccgtcggggc cggcggtgat
     2161 ggccttgtgc gccatattat agaacaacga gtgttcgagg atgccgggga tcgtagtgac
     2221 gcgatcgttg agtatcccgg gttcggggaa gctgtcgaac cagttgtcga tgaggaaatt
     2281 tcccctttcg gtgatgacgg cgccgtcctt tttgtcgctg agccggagtt cggagcgcgt
     2341 cgggttgaag tattgcttta gcctggcgga tacgtacgcc agggcttcgg ggatgacctc
     2401 gacgacgagt gggtaggtgt tgctaaggcg atcgacgcgt ttggccgcat cgccgacgag
     2461 aacgaacgta tcggccatgg cggcgaggac cttttccgcc gtatggacgc cgccgccgct
     2521 tttgagggcg gtgagccggc ggtcgaactg gtcgcagccg tcaaagtaat agtccagccg
     2581 ggagagccag ccggagtcct ttacgttgaa gccttgctgc aggagggtct ggcgggtggc
     2641 gaaggaggag ctggccacgg tgagcgtttg cacgagaccg ggggtgtctt ttatcgccgt
     2701 gatgagatgg gtcatggtgg agccggcgcc gagaccgatg gcgtggccgg gttggatgag
     2761 tttggcggct tcctgggctg cctgttgttt atagtcgggc atggttattt gttggttagt
     2821 ttgttggttg gttagtttgg tgtttgggtt tctcggctgg gccggatcgg gatgtccggt
     2881 ccggcgcggc gcattccctt gctttggctt tttggatcgt tggagtcaac catgcgaggc
     2941 accttgcccg gcatcccgac accggcctgg tccaagagga ggcaagggtt attctattca
     3001 gcatactttt ttgcgatggt gtcgtaaaag ttttttagtg tgaagaaggc gcgctttttt
     3061 acgccctgct cggagatgag gcccttgcgg ttccagaaat tctggtagat gggatgctgg
     3121 cggcgggggc tgcggaagtc cgcgaggatc cagggggtta tgccgcggag ggctgtgatt
     3181 cccgacagca tggtgagctg gttggtatag agggcctcct ggtattcttc gctccagcgg
     3241 gtgtcggggt cggcgtggaa gccggcgagg gcgtcgccac cgaactcggt gatgacgacg
     3301 ggccgatcgt atttgatggt aaaatgatag ttgtgcagat cggtggg
//
LOCUS       DATGFG010000108         3099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_437244, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000108 DATGFG010000000
VERSION     DATGFG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437244"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /gene="rmuC"
                     /locus_tag="VKQ52_02030"
     CDS             complement(<1..1228)
                     /gene="rmuC"
                     /locus_tag="VKQ52_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HLZ85983.1"
                     /translation="MAGLTIAGILGFLLLGALIGFVLTWFWARARHAESRAAASAELT
                     LARAANKALEEKLQGQTREIEDLNRKFSTEFENLANRILETKAERFTQLNKANLEALL
                     NPLGKTITEFREQVNKVYDAESKERFSLGVKVRELAQLNQQLSEDAKNLTRALKGEAK
                     TQGRWGEVILESILERSGLRKGEEFFMEYQLYDAEGRPLRNEAKGSRMRPDAIIKYPD
                     DRHVIIDAKVSLNAFVRFVETSDAEQQKLELAAHVRAIKEHIHDLNAKAYDDYHKTLD
                     FVMMFIPSEAAYIAALQGDADLWNYAYDRRILLINPTNLIVSLKLIVDLWKREYQNLN
                     AQAIADRGAKLYDKFAGFLKNLQGVGQALDTAQNSYQDAWKQLSTGKDNLVSQAIKLK
                     NLGVKTKSELPLNLPEP"
     gene            1286..1699
                     /locus_tag="VKQ52_02035"
     CDS             1286..1699
                     /locus_tag="VKQ52_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85984.1"
                     /translation="MSSKVFFYCLKVWLTSVVLGPVIFWCWEIPDILTLSSFLAYLGI
                     AILYGLVFSLLSFLLLWAGIAFLYRRGWPLTRQRLAAACWGLTLTLVPFLVLFARADS
                     VMQPDSVHLSLSYLFPILVGIFVYQWPGRRPRTER"
     gene            1703..2272
                     /locus_tag="VKQ52_02040"
     CDS             1703..2272
                     /locus_tag="VKQ52_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ85985.1"
                     /translation="MELLREHIGKIVALTDGQFDYFLAHFQRQSFKKGQMIIGEGDRV
                     DKEYFVLSGCLKSFFVNDEAKMYILQFSMPTWWASDYNALYNGTRASINIDCITDAEV
                     LSITNSDREKLCSELHPIEHFFRWRTNRGYVATQKRLLSLMDNDAKARYEELLKMYPE
                     LYNIVPKHLIAAYLGVSRETLSRLYHAAK"
     gene            2278..>3099
                     /locus_tag="VKQ52_02045"
     CDS             2278..>3099
                     /locus_tag="VKQ52_02045"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HLZ85986.1"
                     /translation="MWNDTKIAKLLGVEYPIFQGPFGGNLSTVTLAAMVSNLGGIGGY
                     GAYTNTPDEIVKVNTELRKTTNKPYNINLWVSDNDVPAGSVTEEELQRVKRLFQPYFE
                     ELGIPLPGKPLPFSSRFENQVEVILDVRPPVFSFMFGTPSAAILEQCRRAGIVTVGAA
                     TTLDEAVVLESAGVDAIIASGFEAGGHRPSFLAPAEQSLTGTFVLLQQIKEKVQTPVI
                     AAGGIATGRGIAAALALGADAVQVGTAFLACEESNAPAIHREMLFGAESRYTVLTR"
BASE COUNT          564 a          834 c          921 g          780 t
ORIGIN      
        1 ccggttcggg taagttcaac gggagttcgc tttttgtttt cacaccaagg tttttgagct
       61 ttatcgcctg ggacacgagg ttgtccttgc cggtgctgag ttgtttccag gcatcctggt
      121 agctgttctg cgcggtgtcg agggcctggc cgacaccctg caggtttttc aggaagccgg
      181 cgaatttgtc gtagagtttg gcgccacggt cggcgatggc ctgggcgttg aggttctggt
      241 attcgcgctt ccagagatcg acgatgagct tgagggagac gatcaggttg gtagggttga
      301 tgaggaggat acggcggtcg taggcgtagt tccagagatc ggcgtcgccc tgcagggcgg
      361 cgatgtaggc ggcctcgctt gggatgaaca tcatcacgaa atcgagggtc ttgtgatagt
      421 cgtcgtaggc ctttgcgttg agatcgtgga tatgttcctt tatcgcgcgg acatgcgccg
      481 ccagttcgag cttttgctgt tcggcgtcac tggtctcgac aaagcggacg aaggcgttga
      541 gggaaacctt ggcatcgatg atgacatgcc ggtcgtcggg gtatttgatg atcgcatcgg
      601 ggcgcatcct gcttcctttc gcttcgtttc gcaagggcct gccttccgca tcgtacagct
      661 ggtattccat aaagaactcc tctccttttc gcaggccgga cctttccagg atggattcga
      721 ggatcacttc gccccagcgg ccctgggtct tggcttcccc cttgagcgct cttgtgagat
      781 tctttgcgtc ctcgcttagt tgctggttca gctgggcgag ctctctaacc tttacgccga
      841 gggagaaccg ctccttcgat tcggcgtcgt agaccttgtt gacctgttcg cggaactcgg
      901 ttatcgtttt gcccagcggg ttcaggaggg cctccaggtt ggccttgttc agctgggtga
      961 accgttcggc ctttgtttcc aggatgcggt tggccaggtt ctcgaattcc gtgctgaact
     1021 tcctgttcag gtcttcgatc tcccgggtct gcccctgcag tttttcttcc agcgccttgt
     1081 tagcggcccg ggccagggtc agttcggctg aggcggctgc ccgggattcg gcatgccggg
     1141 cccttgccca aaaccaggtg agcacgaagc cgatcagcgc acccagcagg aggaagccga
     1201 ggatccccgc tattgttaat cccgccatct gtattggttt atttattata tttagtcaga
     1261 ctacaagaag acttaaagat acattatgtc ctcaaaagta ttcttttact gtctgaaggt
     1321 ctggctgacc agcgtagtgc tgggtcccgt catcttttgg tgctgggaga tccccgatat
     1381 tcttaccctt tcttcctttc tcgcttatct gggtatcgcg atcctgtatg ggctcgtctt
     1441 ctccctgctc agttttttgt tgttatgggc cggtatcgcc tttctttatc gccggggctg
     1501 gccgcttacc cggcaacggc tggcggcggc ctgttggggg cttacactga cgctggtgcc
     1561 cttcctggtc ctgttcgcca gggcggattc cgtcatgcag ccggacagtg tccatctttc
     1621 gctgagttat ctatttccga tcctggtcgg tatcttcgtg taccagtggc cgggcagaag
     1681 gccccgcacg gaaagataga ctatggaact attacgggaa catatcggca agatagtcgc
     1741 gctcaccgac gggcaattcg actatttcct ggctcatttt caacgccaat ccttcaagaa
     1801 aggacagatg atcatcggtg aaggcgacag ggtggacaag gaatattttg tgctctccgg
     1861 ctgtctgaag tccttttttg tcaacgacga ggcgaagatg tatatcctcc agttctccat
     1921 gcctacgtgg tgggcctcgg actacaacgc gctttataac gggacgcgcg cgagcatcaa
     1981 tatcgactgt atcacggacg ccgaggtgtt gtccatcacc aatagcgacc gggaaaagct
     2041 atgcagcgag ctgcatccca tcgaacattt tttccgctgg cggaccaaca gggggtatgt
     2101 ggccacacaa aaacgacttt tgtcccttat ggataacgac gcgaaggccc gttatgaaga
     2161 gctgctcaaa atgtatcccg agctctacaa tattgttccc aaacatctta ttgccgccta
     2221 tctcggggtt tcgagggaaa cgctgagcag gctttatcac gccgccaaat agacccgatg
     2281 tggaatgata ccaaaatagc gaaactcctc ggtgtggagt acccgatctt ccaggggcct
     2341 tttggcggta atctttcgac ggtgacgttg gcggccatgg tctccaatct cggtgggatc
     2401 ggcggttatg gcgcttatac caacaccccg gatgagatcg tcaaggtcaa caccgaactg
     2461 cggaagacca cgaacaagcc ctacaatatc aacctatggg tatccgacaa cgatgttccc
     2521 gcggggagcg tcacggagga ggaactgcag cgggttaagc ggctgttcca gccgtatttc
     2581 gaggagttgg ggatcccgct gcccgggaag cccttgcctt tttcctcccg tttcgagaac
     2641 caggtggagg tgatcctgga tgtccggcca cccgttttta gctttatgtt cggcactcct
     2701 tcggccgcca tcctggaaca atgccggcgg gcgggtatcg tgaccgtcgg agcggccact
     2761 acgctggacg aggcggtagt tctcgagtcg gcgggggtag atgcgatcat cgcctcgggg
     2821 ttcgaggcgg gtggccaccg gccctcgttc cttgcccccg cggagcagtc gctcaccggg
     2881 acctttgtac tgttgcaaca gataaaggag aaggttcaga cgcccgtgat cgcggcgggt
     2941 gggatcgcga caggcagggg gatcgcggcg gcgcttgcgc ttggcgccga tgccgtacag
     3001 gtgggaacgg cctttctcgc ctgcgaagaa tccaatgccc cggccattca ccgggaaatg
     3061 ctttttggcg cggagtcgcg ctatacggtc ctgacgcgg
//
LOCUS       DATGFG010000109         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_437159, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000109 DATGFG010000000
VERSION     DATGFG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437159"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VKQ52_02050"
     CDS             <1..1008
                     /locus_tag="VKQ52_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ85987.1"
                     /translation="FDAGMLVMTDASNDLDHNYPALKNDLLRTGLVTSVTRATAPATD
                     LYSWTGVDDWQGKNPGETLGMATVGITDDYFSTLGMQIIKGRDFAKGMTQDSADVILN
                     EAAVKRLRFKEPLNQVIFWNDRKKIRVIGVVRDALMRSPFSLPEPIFFVYNPYWSRSI
                     MYRLSPRADAGTALSTIAGIFNRYNPAYPFQYHFADEQYAAKFGQEMLIGKLSGIFAT
                     LAILISCLGLFGLAAYTAQQRTREIGIRKILGATDGQVLVLISREFVLLVFFSCMVAS
                     PVAYYFMQGWLRAYYYRTTITADVFIESAAAALLITILTIGFQAIRAVRLNPVDSLRT
                     E"
     gene            complement(1016..1738)
                     /locus_tag="VKQ52_02055"
     CDS             complement(1016..1738)
                     /locus_tag="VKQ52_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLZ85988.1"
                     /translation="MNPDYSKKATSQQIRERFDKDTERFSNLETGQQTTIDAPLTMEL
                     CTEAARYANPDATELLDIGCGAGNYTLKMLGKIPDLNCVLNDLSMPMLEKAKERVSPH
                     TKGTVTVIQDDMRNLHLPDNHFDIILAAATLHHLRDDKDWESMFTKVYKALKPGGSFW
                     VSDFITHHSEIITRLFEDKYSGYLETLGGPEYRQKVLDYVQYEDTPRSLNFQLALLAK
                     VGFSKIDILHKNACFGAFGAIK"
     gene            complement(1892..2626)
                     /locus_tag="VKQ52_02060"
     CDS             complement(1892..2626)
                     /locus_tag="VKQ52_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ85989.1"
                     /translation="MTTTAQLLTGKRVMLLGGSSGIGLATAKKAAAEGARVVIVSGNR
                     HRVDQALKELPAGSEGYAVDLSKEEKIRDFFSSTGRFDHLVYSAGENLVLSPVAQTEI
                     RQAQEFFTIRFWGAFAAVKYAGPLINEGGSVGLTSGTASIRPGAGWSVASAICGAMEG
                     FVRALAVELSPIRVNCVVPGVIRTPLWDSMGPLERDELFHQVSRSSLVKRVGHPDDVA
                     EGFLYLMKQRNGTGQSLLIDGGATLV"
     gene            complement(2699..3277)
                     /locus_tag="VKQ52_02065"
     CDS             complement(2699..3277)
                     /locus_tag="VKQ52_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ85990.1"
                     /translation="MESLITYIMSLRPLTGEEKTEITSHFQAGHFPEGDYLFRGGRVC
                     TKLFFIVSGVVRLVAVNDKGVEVTHYFVGENQFCTNLASFTNETVAEDSIQCCSPAVI
                     LTIQKSQLLALYRKLPFMEDLIGQVNQQRLLEKIRLKNIYSGEDSAGRYRLFLSEQPE
                     IAGRVPLSHVASYLNITPQSLSRIRRQMKSSI"
     gene            3527..>3802
                     /locus_tag="VKQ52_02070"
     CDS             3527..>3802
                     /locus_tag="VKQ52_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85991.1"
                     /translation="MLDELSYDRFNTQADRIYRIDEQVKFGDFNYNGTEVPAVMGPGF
                     AKDFSQIAGYTRLKYNSNVIIRKGGENIRESKAAYADSSLFDVFTLEI"
BASE COUNT          903 a         1041 c          942 g          916 t
ORIGIN      
        1 tttgacgccg gcatgctggt catgaccgat gccagcaacg acctcgacca caactatccc
       61 gctttgaaga acgatctgtt acgcactggc ctggtaacca gcgttacccg ggcgacagcc
      121 cctgctaccg atctgtactc atggacaggt gtcgacgact ggcagggaaa gaaccccggg
      181 gaaacacttg gcatggcgac tgtaggcata accgacgact atttttcgac actgggcatg
      241 cagatcatca agggcagaga ttttgccaaa gggatgaccc aggactcggc cgacgtgatc
      301 cttaacgagg cggcggtcaa acgactgcgt ttcaaagagc ccctgaacca ggtcattttc
      361 tggaacgaca gaaaaaagat ccgcgtgatc ggcgtcgtca gggatgcgtt gatgcggtct
      421 ccattttcat tgccggaacc gatatttttc gtgtacaacc cctactggtc gagaagtatc
      481 atgtaccggc tgtcgccccg ggcagatgca ggtacagctt tgtcgaccat cgccggcatt
      541 ttcaatcggt acaaccccgc ttatcccttt caataccatt ttgccgacga gcaatatgcc
      601 gcgaagttcg gccaggagat gctcatcggt aagttatccg ggatattcgc gactctcgcc
      661 attttgatat cctgcctggg cctctttggc cttgccgcct atacggccca gcagcgaacc
      721 agggaaatcg gcatccgtaa gatccttgga gctaccgatg gacaggtgct ggtgctgatc
      781 tcgagggagt tcgtattgct ggtcttcttt agttgcatgg tggcctcgcc ggtggcgtac
      841 tatttcatgc agggctggct gcgggcctat tattaccgaa caaccatcac agcagatgta
      901 ttcatcgaat ccgccgccgc tgccctcttg attaccatac tcaccatcgg tttccaggcc
      961 atacgggcgg tgcgattgaa tcccgtcgac agcctcagga cggaataggg aaccgctatt
     1021 ttatcgcgcc aaacgcacca aagcacgcgt tcttgtgtaa tatatctatt ttactgaatc
     1081 caaccttcgc cagcaaagca agctgaaaat tcagcgatcg gggagtgtcc tcatactgta
     1141 catagtccag tactttctga cggtattcgg ggccccccag cgtttccaaa taaccgctat
     1201 acttatcctc gaatagcctg gtgatgatct cggagtgatg cgtgataaag tcggacaccc
     1261 aaaagctgcc tcctggtttg agcgctttat atactttagt gaacatcgat tcccagtcct
     1321 tgtcatcgcg caggtggtgc agggtggccg cagcgagaat aatgtcaaaa tgattgtccg
     1381 gcaggtgcag gtttcgcatg tcatcctgga ttacagtaac cgtacccttc gtatggggcg
     1441 agaccctctc ctttgctttt tccaacatag gcatgctcaa atcattcaac acgcagttca
     1501 ggtcggggat cttgcccaac atcttcaggg tgtaattacc cgcgccgcaa ccgatgtcca
     1561 gcagctccgt ggcatccggg ttggcatacc ttgccgcttc cgtgcacagc tccatggtta
     1621 gcggtgcgtc gatggtggtt tgctggccgg tttcgaggtt ggaaaaacgt tcagtgtcct
     1681 tgtcgaatct ctccctgatc tgttggctgg tcgctttttt tgaataatcc gggttcatac
     1741 caaaactttg atttggtgaa gttaccgggt ttagccaaat ttgaaagtat caatttcatc
     1801 atgtgttgat actctacagg tatggaacta agatcgaatt ggaaaataaa cccgactggc
     1861 ctgggagtca tcccggccag ggccggacag gctataccag cgtcgctccg ccgtctataa
     1921 ggagcgactg tcccgttccg ttacgctgct tcatcaggta gagaaatcct tccgctacgt
     1981 catccggatg gccgactctt ttaaccaggg aactcctgct cacctggtga aaaagctcat
     2041 cccgctccag cggccccata ctgtcccaga gcggcgtcct gatcactccc ggcaccacac
     2101 agttcacccg tatcggtgaa agttctacgg ccaacgcccg gacaaaaccc tccatcgccc
     2161 cgcaaatagc ggaagccacc gaccaaccgg cgccaggccg gatactggcc gtgccgcttg
     2221 tcaatccgac ggatcctcct tcgttgatca gcggaccggc atatttcact gcggcaaaag
     2281 ctccccagaa gcggatcgta aaaaactcct gcgcctgtct tatctctgtt tgggccaccg
     2341 gcgaaaggac caggttttcc cctgccgaat acaccaggtg gtcaaagcga cccgtggaag
     2401 aaaaaaaatc cctgatcttt tcctctttgc tcaagtcaac tgcatagccc tcgctgcctg
     2461 ccggcaattc tttcagggcc tggtccaccc gatggcggtt gccggagaca atcacgacgc
     2521 gggcaccctc cgcggccgcc ttcttcgcgg tcgccaatcc aattccggaa ctgccgccaa
     2581 gcagcataac cctcttccct gttaacaatt gtgctgttgt agtcatagct cgtctgtttt
     2641 agggcaaagg tcctgagata gagcgagccg gcgattacca aatggtaaat agtggaacct
     2701 atatggacga tttcatctgc cggcgaatcc tgcttaaaga ctggggcgta atattcaggt
     2761 aggaggccac atggctcagt ggaacacgac cggcaatctc cggctgctcg ctgagaaaga
     2821 ggcggtagcg gccggccgaa tcctcaccgg aatagatatt cttcagccgg atcttttcca
     2881 gcaaccgctg ctgattcacc tggccgatca ggtcttccat gaagggaagt ttcctgtaaa
     2941 gcgccaatag ctgcgacttt tgaatcgtca aaatcactgc ggggctgcaa cactggatgc
     3001 tgtcttcggc gacagtctcg tttgtaaaac ttgccaggtt ggtacagaac tgattttcgc
     3061 cgacaaaata atgggtcacc tcgacgcctt tatcgttgac ggccaccagc cggacaacac
     3121 cggaaacgat aaagaaaagc ttagtgcaca cacgaccgcc cctgaaaaga taatcgcctt
     3181 ccgggaaatg accggcctga aaatgtgagg tgatctccgt cttttcctct ccggtgagtg
     3241 gcctgaggga cataatatag gtaatcagcg attccatgtc accccacaat ttaagcccat
     3301 attgaataac gatcacgtaa aaaagcatac catttgtgta accatatgtc gggcacatcc
     3361 gtccaatccg ttttttacct gacaaatact aataacacca gctcttatga ttaaaaacta
     3421 tttcaaaacg gcctggcgga gcttgaggaa gaacaaaacc ttcagcattc tgaatatcat
     3481 aggattatcg attggcgtgg cttgttccct tttaatcgct ttatatgtgc tggatgagtt
     3541 aagctacgac aggttcaata ctcaggctga tcgtatttac cggatcgatg aacaggtgaa
     3601 atttggcgat ttcaattaca atggaacaga ggtgcccgcg gtaatgggac ccggttttgc
     3661 aaaggatttt agccagatcg cgggatacac ccgcttgaaa tataactcca atgtcattat
     3721 ccggaaaggc ggcgagaaca tccgggagag caaggccgcc tatgccgact cctcgttatt
     3781 cgacgttttt accctggaaa tc
//
LOCUS       DATGFG010000110         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1463674, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000110 DATGFG010000000
VERSION     DATGFG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463674"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..>2181)
                     /locus_tag="VKQ52_02075"
     CDS             complement(117..>2181)
                     /locus_tag="VKQ52_02075"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HLZ85992.1"
                     /translation="KLVVDVAASPFFTPGMSALDVLERSPGISIDPMTSLISLNGRAG
                     TVVYINGRHSYLSGQDLLNYLRGLPAGTLDQIEIMSQPPAKYDAAGSAGVINIIFKKN
                     QADGVYGSVTASGLYGFYFRTRDNLLLDWRKDKVNINFSYGVTDNKGFTDQHMLSSFR
                     TDYGIPFSQYQDNFTSTITTPVSHTPLLSVDYQASKTTSLGANLTGLFTNSHSVTDGP
                     ILLYDSLGRLVQHTDFMSGTRNPVAHYGINLNLQQKLDKKGREISADADYFYYHSPGS
                     QYSDNYSHLADGSAQAPYLVNGDIPSQVDIYAFKADYDQPLRLGKGAKLEAGVKSSYV
                     RTDNDARYTQYDTTLGKWQPDAGLTNHFIYTENINAAYLTVSKELNKKWSAEIGVRGE
                     QTISKGDERVQESRFSRDYFQLFPTAYIKYSPNGNHDFNLSYGRRMDRPNYQDLNPFR
                     FVLNQYSVREGNPALQPEYVNNFEFSYNYKSDITVWLDYIKFDNLFARVIKSEGQGND
                     WITVQTKENVAFRRNIFFFLFYHKALVKWWNVNWLTGLINARLSDPANTGDQADQITV
                     FRLQMGNQFSLGGGWSVDLRGDYISRRLEGIRIYALPSGSISAGVNKKVLKNKGTVTA
                     FVNDIFDQFRPGQTSEAAGFVMKTTNHPETRYLTLTFNYRFGKQRQQRQWNAGSEDEQ
                     RRVNF"
BASE COUNT          512 a          649 c          515 g          505 t
ORIGIN      
        1 gcccgtatgc tgaaaggcga cgtccgctat cgcttcgtta tcgacatcgc aacgatcaaa
       61 tagaacatgc acccgaaaga atgataaagc cccggtctga cagccggggt tttttattag
      121 aaattcacgc gccgctgctc atcctccgaa cccgcattcc actgccgttg ctgccgctgc
      181 tttccaaaac gataattgaa ggtcagcgta agataacggg tctccggatg gttcgtcgtc
      241 ttcataacaa aacccgccgc ctcactggtc tgccccgggc ggaactggtc aaaaatatca
      301 ttcacaaagg ccgtcacggt ccccttgttc ttcaacacct ttttgttgac ccccgcagaa
      361 atggaccccg acggcagcgc gtaaatgcgg atcccctcca gccgccggga aatataatcc
      421 ccccgcaggt caacgctcca tcctcccccc aacgaaaact ggttacccat ctgaagccgg
      481 aacaccgtta tctgatccgc ctgatcgccc gtattggccg ggtccgaaag cctggcgttg
      541 atcaggcccg tgagccaatt gacattccac cactttacca gcgccttgtg ataaaagagg
      601 aagaaaaaga tattccggcg gaatgcaaca ttttccttcg tctggaccgt gatccaatca
      661 ttcccctggc cctcgctttt tatcacccgc gcaaagagat tgtcgaattt gatataatcc
      721 agccagacgg tgatgtcgct cttgtaattg taggagaact cgaaattgtt gacatattcc
      781 ggctgcagcg cgggattccc ttcccggaca ctatattgat tgaggacaaa ccggaaggga
      841 ttcaggtcct ggtaattggg ccggtccatc cgccgcccgt agctgaggtt aaaatcatgg
      901 ttcccgtttg gcgaatactt gatataggca gtagggaaca gctggaaata atcccggctg
      961 aaccggctct cctgtacccg ttcatccccc ttcgagatag tttgttcccc ccgcaccccg
     1021 atctccgcgc tccatttctt gttcagttcc ttgctgacgg tcagataggc ggcattgata
     1081 ttctccgtgt atataaaatg attcgtaagc ccggcatccg gttgccactt gcccagcgtc
     1141 gtatcatact gagtataccg cgcatcatta tccgttcgca catagctgct cttgacccct
     1201 gcttccaatt ttgccccctt gccaagccgc agcggctgat catagtctgc cttgaacgca
     1261 tagatatcca cctgcgaagg gatatcgcca ttcacgagat acggcgcctg cgccgacccg
     1321 tcggccagat gagaataatt gtccgaatac tgactgcccg gactatgata ataaaaataa
     1381 tcggcatcgg cgctgatctc ccggcccttc ttatccaact tctgctgcag gttcaggttg
     1441 atcccatagt gcgccacggg attcctggtc ccactcataa aatccgtatg ctggacaagc
     1501 cgccccaggc tgtcatacag caggatcggc ccatcggtaa cactatggct gttggtgaac
     1561 aatccggtaa gatttgcgcc cagactcgtg gtctttgaag cctggtaatc caccgaaagc
     1621 aagggggtat gcgagaccgg ggtcgtgatg gtcgaagtga aattatcctg gtattgcgag
     1681 aacgggatcc cgtagtcggt gcgaaacgaa ctcagcatat gctgatcggt aaagccctta
     1741 ttatccgtca ccccatacga aaaattgata ttcaccttat ccttgcgcca atccagcagc
     1801 agattatccc gggtcctgaa ataaaaccca taaagcccgc tggcggtgac actgccgtac
     1861 accccatccg cctggttctt tttaaagata atattgatca cacccgcact ccccgcagca
     1921 tcgtatttgg caggcggctg cgacatgatc tcgatctgat ccagcgtgcc cgccggaagg
     1981 cctcgcaggt aattcagcaa atcctgaccc gacaggtagc tatgtctccc attgatatag
     2041 acaacagtac cggccctgcc gttcaggctg atcaggctgg tcatcggatc gatgctgatg
     2101 cccgggctcc gctccagcac atccagggcc gacatcccgg gagtaaaaaa gggcgaagcc
     2161 gctacatcca ccaccagctt g
//
LOCUS       DATGFG010000111         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_891822, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000111 DATGFG010000000
VERSION     DATGFG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891822"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2207)
                     /locus_tag="VKQ52_02080"
     CDS             complement(<1..2207)
                     /locus_tag="VKQ52_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ85993.1"
                     /translation="MFRNYWKVAVRSLLKRKGFTLINILGLATGMAVCLLILLFVRHE
                     LSYDQWETNGDRIYRVVLERKYPERSTSYAIIPFSIGPAIRREFPEVEEATGLGDFTG
                     TDAMLVRIGDRRFEERNVLTVDSTFFRVFPMNLLQGDAATALEKPGMAVLSEGTAVRL
                     YGSVADAMGKEFETDAQQRFRVTGVCGDLPENSHLKFNMLLTVTSFPFARDVNYTGFS
                     TYTYLLLRPNVSAAALEARLPQIVKKYVAGDIARNFGMSFERFTAAGNGYHYYLQPLQ
                     DIHLDSDLEAELKPNGSRRAVTIFGIIAAFILGIAGINFINLSTARSVERAKEVGIRK
                     TFGSEQRSLVFQFLLESVLVSGLSVLLAVVLVVVALPFFNRLSGEELRAASLLTPVTI
                     PLLLAFGGVVGLVAGLYPAFVLSSFQPIKVLKGKFQSNKYGTALRNGLVVFQFAISIV
                     LIICTIVVNRQMGYMLGDKLGFSKDHVIEIERSDLLAAKTLAFRNELAGIAGVGKVGG
                     GSSMPGDGGFFGISWQPVGSRESMTGRGIVVDASFAETLGLSMKEGRFFSRDYPTDSL
                     AVVLNEKAVQALGLKEPVIGARLRTTSEFLNPKPDSFYTYTVVGVVHDFHYQSLHQAI
                     NPLVFTNASKFKDVTGVTAVRIKGDDFAPAIAAIEQKWRQFVPDHPFHYSFLDQTLAA
                     QYQGEQTLQRIFTVFSALAIFIACIGLLGLAAYATQQRIREICIRKVLGAGMGT"
     gene            complement(2227..>3469)
                     /locus_tag="VKQ52_02085"
     CDS             complement(2227..>3469)
                     /locus_tag="VKQ52_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLZ85994.1"
                     /translation="PVCLTRGIGRRHQLTFRNIPLDQEERRGREGRALRQDSALHVGQ
                     NLLLTAFDQREMSAGQLFYTMGSVFAPDTTGGLTGAQSITGFFKAKKAPVYGCLKGSF
                     DKPNDVYVDGRQYSHINPQQAGYNWLTVELHHWKMFDGRMSEGLLYKPENFDSTKKYP
                     IIFYFYERDTRTRYDYIAPQPVRASINIAYFVSNGYLVFDPNIYYKTGQPGEDAYNSV
                     ISAAKYLSRFKWVDTTKMGLQGHSWGGYQVAYLVTRTHSFAAAEAGAPVANMTSAYGG
                     IRWETGISRQFQYERSQSRIGSTLWQRPDLYLKNSPLFRADKVTTPLLMMHNDADGAV
                     PWYQGIEYFSALRRLGKKAWLIEYNGEGHGLTERRDRKDWSIRLSQFFGYYLKGEHPP
                     RWMVEGVPATLKGIDWGLTAE"
BASE COUNT          762 a         1165 c          868 g          674 t
ORIGIN      
        1 gtccccattc ccgcgcccaa caccttccgg atacagatct cccggatacg ctgctgtgtg
       61 gcataggcgg caaggcccag caacccgata caggcaataa agatagccag tgccgaaaac
      121 accgtgaaaa tgcgctgcag cgtctgctcc ccctggtatt gcgccgccag ggtctgatcg
      181 agaaaactat aatgaaaggg atgatccggc acgaactgcc gccacttctg ctcgatcgcc
      241 gcgatggccg gcgcaaaatc atcccccttt atccgcaccg ccgttacccc cgttacatcc
      301 ttgaacttcg acgcattcgt aaaaacaagc gggttgatcg cctggtgcag gctttgataa
      361 tgaaaatcat gaaccacccc caccacggta taggtgtaaa aagaatccgg cttcgggttc
      421 aggaactcac tcgtggtcct gagccgcgca ccgatcaccg gttccttcaa cccaagggcc
      481 tgcactgctt tttcattcag caccacggca agggaatcgg tgggatagtc cctggagaag
      541 aagcgtccct ccttcatgga taacccaagc gtctcggcaa aagaggcgtc cacgacaata
      601 cccctccccg tcatcgattc ccgcgatccc accggctgcc aggagatccc gaaaaagccc
      661 ccgtcgcccg gcatgctcga cccgcccccc accttcccga cacccgcaat acccgccagc
      721 tcattgcgga aggcaagcgt cttggcggca agcagatccg aacgctcgat ctcgatgaca
      781 tggtccttgg aaaaaccaag cttatcccca agcatatacc ccatctgccg gttgacaacg
      841 atggtacaga tgatcagtac gatagatata gcaaactgga aaacgacaag cccgttacgc
      901 aacgcggttc catatttgtt ggattggaac ttccccttca ataccttgat gggctgaaag
      961 gacgacagca caaacgccgg gtaaagccct gcaacaagtc ccacgacacc accaaaagcc
     1021 aaaagcagcg ggatcgtcac cggcgtcagc aggctcgccg ccctaagctc ctcccccgaa
     1081 agccggttga agaagggaag cgccaccact accagcacga cagccagcag gacgctaagc
     1141 ccgctcacca gcaccgattc cagcaggaac tggaagacaa gactccgctg ctccgaacca
     1201 aaggtcttgc ggatacccac ctccttggcc cgttcaaccg atcgtgcagt ggacagattg
     1261 ataaaattga tccccgcgat ccccaggata aaagcggcga tgatcccaaa aatggtaacg
     1321 gcccgccggc tcccgttcgg tttgagctcc gcctccaggt ccgaatcgag atggatatcc
     1381 tgcagcggct gcaggtagta gtgatacccg ttccctgctg cggtgaaccg ctcgaagctc
     1441 attccgaaat tgcgggcgat atcccccgcc acatactttt tgacaatctg cggcagtcgc
     1501 gcctccagcg ccgcagccga cacattcggt cgcaaaagaa gataggtata ggtggaaaaa
     1561 ccggtatagt tgacatcccg tgcaaatgga aagctcgtca ccgtaagcag catgttgaat
     1621 ttcagatggg agttctccgg cagatcgccg caaaccccgg ttacccggaa acgttgctgc
     1681 gcatccgtct cgaactcctt tcccatcgcg tccgccaccg acccatacag ccggaccgcc
     1741 gtaccctcgc tgagcaccgc catccccggc ttctccagcg ccgtggccgc atccccctga
     1801 agcaggttca tcgggaagac ccggaagaag gtactgtcca ccgtcagcac attccgttcc
     1861 tcgaaacgcc ggtcgccgat ccgcaccagc atcgcatccg tccccgtgaa atcccccagc
     1921 cccgttgctt cctccacttc cgggaactcc ctccggatgg ccggacctat cgaaaaaggg
     1981 attatggcat aggacgtcga ccgctcggga tacttccgct ccagcaccac ccggtatatc
     2041 ctgtccccgt tcgtctccca ctggtcgtag ctcagctcat gccggacaaa caacaggatc
     2101 agcaggcaca cggccatccc tgtggccagc ccgagaatat tgataagggt aaaacccttc
     2161 ctcttcagca ggctgcggac agccaccttc cagtagtttc tgaacatatc gatagttttt
     2221 gttttcctat tccgccgtca atccccaatc gatccccttc agcgtcgccg gcaccccttc
     2281 caccatccag cgcgggggat gctccccctt caggtagtag ccgaagaact gtgatagccg
     2341 tatcgaccag tccttccggt cccgccgctc cgtcagccca tgcccttccc cgttatactc
     2401 gatcagccag gccttcttgc caagacggcg aagcgcggag aagtattcga tcccctggta
     2461 ccacggcacc gccccatcgg catcgttgtg catcatcagc aggggcgtcg tcaccttgtc
     2521 ggcccggaac aggggggagt tcttcaggta gagatccggc cgctgccaga gtgtgctgcc
     2581 gatacggctc tgcgatctct catactggaa ctgccggctg atccccgtct cccagcggat
     2641 acccccataa gcgctggtca tgttcgccac cggcgcaccc gcctcggccg cggcaaaaga
     2701 atgcgtccgc gtcacgagat aggccacctg gtagccgccc cagctatgcc cctgcaggcc
     2761 catcttcgtc gtgtccaccc atttgaaacg cgagagatat ttcgccgccg agatcaccga
     2821 attatacgcg tcttcccccg gctgccccgt cttgtagtag atattcgggt cgaacaccag
     2881 gtaaccgttg ctgacgaaat aagcgatatt gatcgaggcc ctcaccggtt gcggcgcgat
     2941 ataatcatac cgcgtccggg tatcccgctc gtagaagtaa aagatgatcg ggtatttctt
     3001 cgtgctgtcg aaattctcgg gcttgtacaa caaaccctcg ctcatccgcc cgtcgaacat
     3061 cttccagtga tgcagttcca ccgtcagcca gttatagccc gcctgctgcg ggttgatatg
     3121 gctgtattgc cgcccatcca catagacatc attgggcttg tcaaaactcc ccttgagaca
     3181 gccgtacacc ggcgccttct tcgccttgaa gaacccggta atgctctgcg ccccggtaag
     3241 cccgcccgtc gtgtcggggg cgaatacgct tcccatcgtg tagaagagct gtcccgccga
     3301 catctcccgc tggtcgaagg cggtcagcag gaggttctgc cccacatgca gcgcggagtc
     3361 ctggcggagg gccctcccct ctcttcccct gcgttcctcc tggtcgagcg gaatattgcg
     3421 gaaggtgagc tgatgccgcc tgccgattcc gcgggtcagg cacacgggc
//
LOCUS       DATGFG010000112         6701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_205354, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000112 DATGFG010000000
VERSION     DATGFG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205354"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VKQ52_02090"
     CDS             complement(<1..748)
                     /locus_tag="VKQ52_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ85995.1"
                     /translation="MNSSPDPPACSRPSCLIVDDEPVARRGLAEDLAAMGLVSVEGMA
                     ADAIQARVLLEAAPVDILFLDIEMPGINGLEFLRGLRTKPLVILVTAWPQYALNGYEY
                     GVVDYIVKPVPLERLRAACEKAVEQHRLRRGGERRHQDPGSGYPLPGSDQLTPGSGAP
                     NLGSGQLFPGSGQHQPQAGSGQTHLFLKVDGAYERVAVADILYVEAANNYIRVHTAHR
                     KLMIYLSLKGMEEQLPPGNFIQVHKSFLVAK"
     gene            complement(745..1746)
                     /locus_tag="VKQ52_02095"
     CDS             complement(745..1746)
                     /locus_tag="VKQ52_02095"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ85996.1"
                     /translation="MSRHPRLRYYLLFTGCFLVFWFLFKFGGILNPVKALTSTALDTI
                     LLTGVLVATVEWLIPAFFYKAKYVRFGCWLGVLLFLGGSINIIGQLSIQGMSLFTYRA
                     SLARYKDHFFYWFWSDLVAGSYFMLAVIALAGFAIRLAFDRLRSELEVLKGQTNPHFI
                     FNALNTIYYTIDGSNRHARELTESFASLLRYQLYECDLPAVPIEKEMRSIHDYIRLQA
                     QRTADTVTIECSGLDQLSGFSVPPHLLMPLVENCFKHVSRFPDKPNFIELQVSRENGT
                     FLFQARNSFDATRPAGATGIGLTLTRKRLALLGKDQGSLVTAPVDGSFSTMLKLRVI"
     gene            1869..2303
                     /locus_tag="VKQ52_02100"
     CDS             1869..2303
                     /locus_tag="VKQ52_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ85997.1"
                     /translation="MQKLHTFPTLPFAIHLPSSLKSTDMFEEILNTVKQHLAENPEVA
                     NAIPAEQQEAIHNEIAAHVTNAMTPPAAGAAGGGDLLGGLLGKLESAIGSGNPIANAV
                     EGGLVASLTSKFGLSPAVTGAIAASLPGLMQKLMHKQTTTAV"
     gene            complement(2365..3468)
                     /locus_tag="VKQ52_02105"
     CDS             complement(2365..3468)
                     /locus_tag="VKQ52_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HLZ85998.1"
                     /translation="MPKKSTTPNIHALPATPNISALPATPNLSAPSNSLTMPRQFIPF
                     LVLAAVLIAGPAPVRAQQAVPVIFDTDMGPDYDDVGAITMLHAYADSGKVKILATMAS
                     NRYEGVAAVLNVFNTYFHRPDLPIGVPGGNAPDIRDKQHWTDSILANYPHAINHNTDA
                     TYAVPLYRKVLAAQPDHSVVIITVGFLTNLADLLETNPDEYSPLDGVQLVKKKVKLLV
                     SMAGTFPRGKEFNVTSDISAALAVSKDWPTPIIYSGFEIGRRIHTGLPLVNNAKIQHS
                     PVKDVFRISLPQSSEDAQGRMSWDETAVFVAVNGYSPWYSLRHGHMLVDTYGSDTWQD
                     SVDEHERQAHLVEALPASTVQQVINKLMMHQPQ"
     gene            complement(3480..4418)
                     /gene="rbsK"
                     /locus_tag="VKQ52_02110"
     CDS             complement(3480..4418)
                     /gene="rbsK"
                     /locus_tag="VKQ52_02110"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HLZ85999.1"
                     /translation="MSTSILVAGSSNTDMVVKTASLPAPGETVLGGRFFMNPGGKGAN
                     QAVAAARLGGAVALIAKLGDDIFGVQALEHFKKEDIDCRYIRIDKHAASGVALITVDE
                     KGENSIAVAPGANALLEPGDIVRAREAIHAAKIILTQLEIPLPTVEKLVDLAAEAGKR
                     VILNPAPARDLPGDLLSQLFLITPNESEAAILTGIPITDLDSATQAATALAARGVPFV
                     IITLGRNGALVWDGKTATHLPAPPVTVVDTTAAGDVFNGALAVALAEGMSITEAAIFA
                     NHAAALSVTRLGAQSSAPTHAETAAFIEQTRRKPRP"
     gene            complement(4546..5280)
                     /locus_tag="VKQ52_02115"
     CDS             complement(4546..5280)
                     /locus_tag="VKQ52_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86000.1"
                     /translation="MRTPSALLAAIALPAALALLPAASATAQPANHTLAAFSIWKPKE
                     GQAALFEAGYKRHLQWHVSAGDRWNWYAWFILSGPRSGQFVDATFGHAWADLDHRVDP
                     AGDGADNSLHTEPFAEFLTGYKLIRLPFSDVEDSTLLGTRYMRMLTIDVSNAAAATTI
                     IAAAAAALRQKLPGQKLLAYEKLDGGGLGQLVLLLGVNTWDDYGKLAGWQDELSDIDH
                     RNARPVITAITAETLVFRRDMSEPKN"
     gene            5416..6225
                     /locus_tag="VKQ52_02120"
     CDS             5416..6225
                     /locus_tag="VKQ52_02120"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ86001.1"
                     /translation="MIRFRQYIPRRIAHVVKSIWYLELVSETPVYEEEIVPDGHHELI
                     FYLHGRSHHRVRDGAWTQEPGALLASQTLQSHTLRMPAGSKLYGIRFYPHTLYPLLGV
                     PLHLLGHVMLPLDDLFTGDGLAACIDEDVPATFRRLETHLEALLRSAAMDGAGYQYVD
                     HSVRRMLATNGTASIRELIAMSGVTAKHYDELFKKYVGITPKCLCSILKFNTVIHYKN
                     RFPAKTLTECAYEAGYYDQSHLIKSFYQWVGASPGQYFHSQSAISDLFAAL"
     gene            6295..6471
                     /locus_tag="VKQ52_02125"
     CDS             6295..6471
                     /locus_tag="VKQ52_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86002.1"
                     /translation="MDIFEQVAEMRVEEKTWEFVTNLLTHTQHPVNEIALLAGVSEDF
                     VKEVKKSLRKRKKG"
BASE COUNT         1326 a         1791 c         2187 g         1397 t
ORIGIN      
        1 gtttggcgac gagaaaggat ttgtgcacct ggatgaagtt cccgggcggc agctgttcct
       61 ccattccttt cagcgacaga tagatcatca gcttcctgtg tgccgtgtgt acccggatat
      121 agttgtttgc ggcttcgaca tacaggatat cggccacggc gacgcgttcg tatgcgccgt
      181 caactttgag aaacagatgg gtttgcccgg aaccggcttg cggttgatgt tggccggaac
      241 cagggaacag ttggccggaa ccaagattcg gagctccgga accaggggtc agttggtcag
      301 aaccagggag cggatagccg gaaccgggat cctggtggcg ccgttctcct ccccggcgca
      361 gccgatgctg ttccaccgct ttttcacagg cggcccgcag gcgttccaac ggcaccggtt
      421 tgacgatata atccaccacc ccatattcat atccattcag tgcgtattgc ggccacgccg
      481 tgaccaggat gaccaggggc tttgtcctga ggccgcgcag gaattcgagc ccgttgatcc
      541 cgggcatttc gatgtccaaa aagaggatgt ccacgggggc cgcctcgagc aggacccgcg
      601 cctggatggc atcggcagcc atcccctcca cggagacgag ccccatcgcc gccagatcct
      661 ccgcaaggcc gcgtctggct accggttcgt catcgactat cagacaagag ggccgcgaac
      721 aggcaggtgg atcagggctc gaattcatat gacccggagt tttagcattg ttgaaaaaga
      781 gccgtccacg ggagccgtga ccagcgaacc ctgatccttt cccaggaggg ccagacgttt
      841 acgggtaagg gtaagcccga tgccggtggc gccggcgggc cgggtggcgt cgaagctgtt
      901 gcgcgcctgg aacaggaagg tgccgttttc gcggcttacc tgcaactcga tgaaattggg
      961 tttgtcaggg aagcgcgata catgcttgaa gcaattctcc accaggggca tcaggagatg
     1021 cggcggaacg gagaagccac tgagctggtc gagaccggag cattcgatgg taacggtatc
     1081 ggctgtacgc tgggcctgca accggatata gtcgtggata gagcgcattt ccttttcgat
     1141 cgggacggcg ggcaggtcgc attcgtacag ctggtaccgc agcagggagg caaagctctc
     1201 ggtgagctcc cttgcgtggc gattcgaccc gtcgatggta tagtagatgg tgttcagcgc
     1261 attgaagata aagtgcgggt tggtctgccc cttcaggacc tcgagctcgg accggagccg
     1321 gtcgaaggcc agccggatgg caaagccggc cagcgcgata acggccagca tgaaatacga
     1381 gcctgccacg aggtcggacc agaaccagta gaagaaatga tccttgtatc gggcgaggct
     1441 tgcccgatag gtaaagagac tcatgccctg tatggacagc tgcccgatga tattgatgct
     1501 cccaccaaga aagaggagga caccgagcca gcacccgaag cggacgtatt tggccttgta
     1561 aaagaaagcc gggatcagcc attccaccgt tgccacgagg acgcccgtga gcaggatcgt
     1621 atcgagggct gtcgatgtga gggctttcac cgggttgagg atgccaccga atttgaagag
     1681 gaaccagaat acaaggaagc agccggtgaa aaggagataa tagcggaggc ggggatgcct
     1741 ggacatatgg gcttgttctc ccaaggtaag acaaaaaaga ttgtcgtaaa cggccgcgcc
     1801 ggcagattta tgcaagaggt cgtgtttacg gggtaagtgg tgaaatgttt ttggggttga
     1861 tttgcattat gcaaaaactt catacctttc ccacccttcc tttcgccatc catcttccgt
     1921 catcgctaaa atctaccgat atgttcgaag agatcttaaa caccgtaaaa cagcaccttg
     1981 ccgagaatcc ggaggtagct aatgccattc ccgccgagca acaggaagcc atccacaacg
     2041 agatcgccgc gcatgtgacc aatgccatga cgccgcctgc cgccggggcc gcgggaggtg
     2101 gagatttgct gggcggtctg ctcgggaaac tggagtctgc catcggatcg ggtaatccca
     2161 tcgccaatgc cgtggaaggt gggctggtag ccagcctgac gagcaagttc ggtctgtcgc
     2221 ccgcggtcac cggcgccatt gccgcttcgc ttccggggct gatgcaaaag ctgatgcaca
     2281 agcaaaccac cactgccgtg taagcctccc ttttgtagaa gactgtttag ctgtcttact
     2341 gcggcaggtg actattcagc cattttactg cggctggtgc atcatcagct tgttgatcac
     2401 ctgctgtacg gtgctcgcgg ggagggcctc gacgagatgg gcctgtcgtt cgtgttcgtc
     2461 gacggaatcc tgccaggtgt cactgccata ggtgtcgacg agcatatggc catgtcgcag
     2521 gctgtaccag ggggaataac cgttgacggc gacgaatacg gccgtctcgt cccagctcat
     2581 ccggccctgc gcgtcctcgg atgactgcgg caggctgatg cggaagacat ccttcacggg
     2641 gctatgttga attttagcgt tgttgaccag cgggaggccg gtgtgtattc tcctgccgat
     2701 ctcgaatcca ctatagataa tgggcgtcgg ccagtccttc gatacggcga gggccgccga
     2761 gatatcgctc gtcacgttga attccttgcc ccgcgggaag gtacccgcca tggacacgag
     2821 gagctttacc tttttcttta ccagttggac cccgtcgagg ggggagtatt cgtcggggtt
     2881 tgtctcgagg agatcggcga gatttgtaag gaatccgacg gtgatgatga cgacgctgtg
     2941 gtcgggctgc gccgcgagga ccttacggta aaggggaacc gcatacgtcg catcggtgtt
     3001 gtggttgatc gcgtgcgggt agttggcaag gatgctgtcc gtccagtgtt gtttgtcgcg
     3061 gatatcgggt gcgttgccgc cggggacgcc gatcggcaga tcggggcggt ggaaataggt
     3121 gttgaagaca ttcagcacgg cggcgacgcc ctcatagcga ttgctcgcca tcgtcgcgag
     3181 gatcttgacc ttgccgctgt cggcatacgc gtggagcatg gtgatcgccc ccacatcgtc
     3241 atagtcgggc cccatatcgg tgtcgaagat gacgggcacg gcttgctggg cgcggacagg
     3301 ggccgggccg gcgatgagga cggcggcgag gacgagaaac gggatgaatt gccttggcat
     3361 ggtcagcgaa ttcgatggag cagacaagtt gggcgtggcc ggtagggcgg agatgttggg
     3421 ggtggccggc agggcatgaa tgttgggcgt ggtagacttc ttcggcatac aaaacgattt
     3481 tagggacgcg gcttgcgccg ggtttgttcg ataaaggccg ctgtttcggc atgggtggga
     3541 gcagaggatt gggcgccaag ccgcgtcacc gacagggcgg cggcatggtt tgcaaatata
     3601 gcggcttctg tgatactcat cccttcggcc agggcgacgg ccagggcgcc gttgaaaaca
     3661 tcgccggcgg cggtggtgtc gacgacggtg accggtggcg cgggaaggtg ggtcgcggtc
     3721 ttcccgtccc agacgagggc gccatttcgc cccagcgtga tgatgacgaa ggggacgccg
     3781 cgggccgcga gtgccgttgc cgcctgcgtt gccgaatcga gatcggtgat ggggatgccg
     3841 gtgaggatgg cggcttcgct ctcgttcggg gtgatgagga acagttggct gagcaagtcg
     3901 ccgggaaggt cccgcgccgg ggcggggttg aggatcaccc gcttgccggc ctccgccgcg
     3961 aggtccacca gcttttccac ggtgggtagc gggatctcca gctgcgtcag gatgatcttc
     4021 gccgcgtgga tcgcttcgcg ggcccggacg atatcgccgg gttcgagcag ggcgttggcg
     4081 ccgggcgcca ccgcaatgga gttctcgcct ttttcgtcga cggtgatgag cgccacgccc
     4141 gaggcagcgt gtttatcgat gcggatatac cggcaatcga tatcctcttt tttgaaatgt
     4201 tcgagagcct ggacgccgaa gatatcgtcg cccagttttg cgatcagggc cacggccccg
     4261 cccagccggg cggctgctac ggcctggttg gcgccttttc ccccggggtt cataaagaag
     4321 cggccgccga ggacggtctc cccgggtgcc ggcaggcttg ccgtcttgac caccatatcg
     4381 gtattgctgc taccggcgac aaggatggat gtgctcacgc gaaaggaatg tagcgtctaa
     4441 gctacaaatt tatgctcgaa accggatgga atccggtttg tacgcgaggc cccgggccga
     4501 agcggaccga cattgtgaag ggggagccaa aaaattatat ttccgctaat tttttggttc
     4561 actcatatcc cttcggaaca cgagggtctc cgcggtgatg gcggtgatga cgggcctggc
     4621 gttgcggtga tcgatgtcgg agagctcgtc ctgccagccg gcgagtttgc cgtagtcgtc
     4681 ccaggtgttg acgccgagga ggaggacgag ttggccgagt ccgccgccgt cgagtttttc
     4741 ataggcgaga agtttttggc cggggagttt ttgacggagg gccgccgccg ccgctgctat
     4801 tattgtggtg gcggcggccg cattgctgac gtcgatggtg agcatgcgca tataccgcgt
     4861 gccgaggagg gtactatcct cgacgtcgga gaagggcagg cggatcagct tgtatcccgt
     4921 gaggaattcg gcgaagggtt cggtgtggag gctgttgtcg gcgccgtcgc ccgcgggatc
     4981 gacgcggtgg tcgaggtcgg cccaggcatg accgaaggtg gcgtcgacga attggccgct
     5041 gcgcgggccg gataggatga accaggcgta ccagttccac cgatcgccgg cactgacgtg
     5101 ccattgcaga tggcgtttat agcctgcttc gaagagggcg gcctggcctt cctttggttt
     5161 ccagatggag aaggcggcga gggtgtggtt tgcgggctgc gccgttgcgg aggcggcagg
     5221 caggagggcg agggcggcgg gcagcgcgat cgcggcgagg agggcggatg gtgttctcat
     5281 cgtttttttg cggcaaagct atcccgtcga cggcgcgaaa aattgtaaaa aaatgaagtg
     5341 gcggcctacg cggcaattgg gtaaattcgc tgctatgcgc agcgtcctcc accggggagt
     5401 cgcgtgcttt cgcctatgat ccgcttccgg caatatatcc cccgaaggat cgcccacgtg
     5461 gtgaaatcca tctggtacct ggaactcgtc agcgagacgc cggtgtacga ggaggagatc
     5521 gtccctgacg gtcaccacga actgatcttt tatctccacg gccgcagcca ccatcgcgtc
     5581 cgcgacggcg catggaccca ggagccgggg gccctgctcg cgagccagac gctgcaaagc
     5641 cacacgctgc gcatgcccgc gggctcgaag ctctatggta tccgtttcta tccccatacg
     5701 ctctatcccc tgctgggtgt accgctgcac ctgctcggcc acgtcatgct gccgctggat
     5761 gatctgttca ccggcgacgg actggccgcc tgtatcgacg aggatgtgcc ggccaccttc
     5821 cgccgcctcg aaacccattt ggaggcgctg ttgcggtccg cggcaatgga cggtgcgggg
     5881 tatcagtatg tcgaccattc ggtgagaagg atgctggcca ccaacgggac ggcgtcgatc
     5941 agggagctga tcgccatgag cggggtcacg gccaagcact atgacgaact gttcaagaaa
     6001 tatgtcggca tcacgccgaa atgcctttgc tccattctga aattcaatac cgtcattcat
     6061 tacaagaacc gcttccccgc gaagacgctc accgagtgcg cctatgaggc agggtattat
     6121 gaccagtccc acctgatcaa atccttttat cagtgggttg gcgcgtcgcc ggggcagtac
     6181 tttcattcgc agtcggcgat cagtgatctg tttgcggcgt tgtagggaca tttcctatct
     6241 ttgtagcctg cctgcttttc caacctacgt tatatcattc aaaaaacgaa taccatggat
     6301 attttcgaac aagtggcaga gatgagggta gaggaaaaaa cgtgggagtt tgtgactaat
     6361 ctgctcacgc atacacaaca tccggtaaat gagattgctt tactagccgg agtttcggaa
     6421 gattttgtga aggaggtgaa gaagagtctc aggaagagga agaaaggttg atttatatta
     6481 tataatattg aaggccgcct ggtggatcca ggcggctttt tgctttttat atggcggggg
     6541 acgccctact ccgccttcag acttttcacc ggattcgtca gcgccgcctt gatcgtttgg
     6601 aggctcaccg tcgccagcgc gatcagcacc gccgcgaggc cggccgcgag gaagacccac
     6661 cactggatcg agatgcgata cgcgaagttg cgcagccagt g
//
LOCUS       DATGFG010000113         6849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1116497, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000113 DATGFG010000000
VERSION     DATGFG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116497"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="VKQ52_02130"
     CDS             complement(<1..1914)
                     /locus_tag="VKQ52_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ86003.1"
                     /translation="MKKLFTSHGRLVAMLASILFFSLTAHAQVGNPVTGTVLDEKGAP
                     LQGVSVTVQGSTTGTVTDSLGRFTIHVTRGATLVFSYIKYGTQEVPYTNQKTLGITMT
                     PTTTSMNDVVVIGYGTQRKATLTGAVAQIGGAEIVTTKNENIENMLTGKVPGLQILQN
                     TSEPGDFANSISIRGMGNPLIVIDGVQIPDFSVTGGNGDNNVGSSNILTRLDPNDIES
                     ISVLKDASASVYGVKAANGVILITTKKGKSGSLQLSYSGTYGWQVPSGLPKPVNATQY
                     MTLVNQQALHNSNGGILVYDTADFNAYRNGTKKSTDWYDAVFKKHAMQQQHNLTATGG
                     SENTTYLLSAGYTDQDGLLTTNDLNYKRYNVRSNVTSRIGKRITVNLNLSGIMDQKNA
                     PATSFWWTTRETWRELPTQTIYANSTAPYYTNGIVDGGNPVAFENSNIVGYSTQNNIF
                     LNGAISIDYKFPFIDGLTLRALYSYNDQIQDNKLYQKSFNLYTYDAATSTYGATLNGS
                     PSYVQRQYYHYPKNTDQLSVSYAHNFGGIHNVSALLLMEGNEQSADNFAAYRQLSIPV
                     DQIIAGNSALQNATQDGGNTTLYQYATNSFVGRATYDFKGRYLAEFSFRDDKSSKFAP
                     GQGWGFFPSGSVGW"
     gene            complement(2031..4622)
                     /locus_tag="VKQ52_02135"
     CDS             complement(2031..4622)
                     /locus_tag="VKQ52_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86004.1"
                     /translation="MLVKVAKIILLLTLSFPILITNAQGLQFKSEDSLLTQRTSYHVF
                     NSPAPVFHDRFSIAFDLSLWDNANLGYVLNLADKDNSYSLSYLYTNGAGNLNFSIDSK
                     SNKLSIPLPAAFLKKNNWFRVRIDFLLKEDKVIIRIDGNTWQADHLGFKDDMPANLVF
                     GKNQYYTEVPNMAIRDLEIGNGRKSYHFPLNEWSGNTVHDSNGESTGYVENPLWLING
                     SYFWRPVYSQSAKNVAGLNFDPLDQDLFIFTQDSLVTYDPASKRAMAAAYANKIPVNM
                     VLGKSIFNARENKCYIYELFDVAKGAPSVAALNMDRNNLKWSIVGKDALPYQLHHHNI
                     FYDSRQDQFFLFGGYGHYSYHNAFLRYNDTTDRWEHTGFKGDTITPRFFAASGPSDNP
                     DELLLFGGYGNESGSQVVGGKQYYDLYRINLKDHSVKKCWQITPHNEVFVPANNLILS
                     GDKKYFYALCYPHELAATELKLYKFSLSDGSYEVVSAAIPMASLRIETDVNLFYSHKT
                     DEFFCTVQEFSDRNSSTIKVYSLTSPPVSTATYLASLRPTKKPGMAFIFYLFPVFLVV
                     SAALIWFFFGRKKNKRITQTPAQQPAAPPAAEPQAALAAPPLPASPLPAIERNRNAVY
                     LLGEFLAFDRKGKNITHLFSPKIKQLFVLILLHSTGRNGTTSNSPGGTGAGIGSRKIS
                     AKLWPEKDPAKTKNIKGVTFNHLRNIISDIEGIELIFQNDNYSFTIAEPFFCDYCILA
                     GPRQNPETFPDHFRLMTRGPLLQDMPDALMDDFKSSYEERLTTQLLPELKNKYESGDY
                     KWALEIAKLILGMDPFNEEVLKYQLKSYRKWKGIEYSRRAYVQFTQDYERSLGVAYHV
                     SFDKIVQ"
     gene            4783..>6849
                     /gene="ppsA"
                     /locus_tag="VKQ52_02140"
     CDS             4783..>6849
                     /gene="ppsA"
                     /locus_tag="VKQ52_02140"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HLZ86005.1"
                     /translation="MTSYIQKFMGTGMADVAIVGGKNASLGEMLTALSSKGIRVPDGF
                     ATTAEAFRHFLDHNELPARLGVLMSSLDRKSYSNLAEVGAEARKLLYCADMPDDLGHA
                     IAKAYAELCGNGPDSVAVRSSATAEDLPQASFAGQHESFLHISGETAVIDAVQMCFAS
                     LYTDRAIKYREDNGFAHEKVALSAGVQRMVNADKGCSGVCFTLEPESGFRDLIHIAGV
                     WGLGENIVQGEVTPDEFLVFKPLLGKVKNPVIRRTLGAKQMMMIHGTANTGTRHTVNI
                     ETPVALRDRFVLEDDELMQLAQWALLIEGHYGKPMDIEWVKDGNTGELFILQARPETV
                     QSRRNPYLLTEYAVSAKGACLAKGEAVGKNIISGKARVLDSPAEAGQLQGGEVIVTDM
                     TSPDWDPVLKKAAAIVTNKGGRTSHASIVARELGVPAVVGTGDATWKIKDGDLITVSC
                     CEGGTGFIYEGKAEWKEATSDLRKNTLPETVKPMLIVGDPDKAFELSFYPAAGVGLMR
                     MEFIITNFIGVHPMALAHFDTLKAGPVKEKISALTHGYADKSRFFIDRLSEGIAMIAA
                     AFYPREVILRTSDFKTNEYAGLLGGTGFEPGEENPMLGFRGASRYYSPLYKDGFRLEC
                     AAIQRVREDMGLTNLKVMIPFCRTVEEGEKVVALMGEYGLKRGADGLEIYVMAEIPSN
                     VILAEEF"
BASE COUNT         1376 a         1554 c         2286 g         1633 t
ORIGIN      
        1 ccagcccacc gaccccgatg gaaagaagcc ccaaccctgc cccggcgcaa atttggatga
       61 tttgtcatcg cggaagctga actccgccag atatcttcct ttgaaatcat aggtagcccg
      121 gcccacgaag ctattggtgg catactggta gagggtcgtg ttaccgccgt cctgtgtggc
      181 gttttgcagg gcgctgttgc cggcgatgat ctggtctacg gggatggaga gctgccggta
      241 ggcggcgaag ttgtcggcgc tttgttcgtt gccttccatc aggagcagcg cggagacgtt
      301 gtggataccg ccgaagttgt gggcatagct gaccgacagc tggtcggtgt tcttcgggta
      361 gtgatagtac tggcgctgga catagctggg ggaaccgttg agcgtagcgc cgtaggtgct
      421 tgtcgcggcg tcataggtgt acaggttgaa ggatttctgg tagagcttgt tgtcctggat
      481 ctgatcgttg tagctataga gcgcccggag ggtcaggcca tcgataaagg gaaacttgta
      541 gtcgatgctg atggcgccgt tgaggaagat attgttctgg gtgctatagc ccacgatgtt
      601 ggagttctcg aaggcgacgg ggttaccgcc atcgacgatg ccattggtat aatagggagc
      661 ggtactgttg gcgtagatgg tttgggtcgg cagctcgcgc caggtctcgc gggtggtcca
      721 ccagaaggag gtggcgggcg cgttcttctg gtccatgatg ccgctgaggt tcaggttcac
      781 cgtgatgcgt ttgccgatgc ggctggtgac attggaacgg acgttatagc gtttgtagtt
      841 gaggtcgttg gtggtgagga ggccgtcctg gtcggtgtag cccgcgctca gcaggtaggt
      901 ggtgttctcc gagccgccgg tagcggtgag gttatgctgc tgctgcatgg cgtgcttctt
      961 aaacacggcg tcgtaccagt cggtgctctt ttttgtgccg ttgcgatagg cgttgaagtc
     1021 cgcggtatcg tagaccagga tgccgccatt ggagttgtgg agcgcctgct ggttgacgag
     1081 ggtcatgtac tgggtggcgt tgacgggttt tggcaggccc gagggtacct gccagccata
     1141 ggttccggag taggaaagtt gcagtgatcc cgattttcct tttttggtgg tgatcaggat
     1201 gaccccattg gcggccttga ccccgtatac cgaggcgctg gcgtccttca gcaccgagat
     1261 gctttcgatg tcgttggggt cgaggcgggt caggatattg cttgagccga cattgttgtc
     1321 gccgttgccg ccggtgacgg agaaatcggg gatctgcacc ccgtcgatga cgatcaacgg
     1381 gttacccatg ccccggatgg agatgctgtt ggcaaaatcc cccggttcgg aggtgttttg
     1441 caggatctgc agaccgggaa ccttacccgt gagcatgttc tcgatgttct cgttcttagt
     1501 ggtgacgatc tccgcgcccc cgatctgggc gacggcgccg gtgagggtag cctttcgctg
     1561 ggtaccgtag ccgatgacca ccacatcgtt catgctggtg gtggtcgggg tcatggtgat
     1621 gccgagggtc ttctggttgg tgtagggaac ctcctgggtg ccatatttaa tatagctgaa
     1681 gaccagggtt gcgccgcggg tcacgtggat ggtgaatctt cccagggagt cggtgacggt
     1741 gccggtggtg gagccttgta ccgtaacgga aacgccctgg aggggggcgc ctttttcgtc
     1801 cagtaccgtt ccggtcacgg ggttgccgac ctgggcatga gcagtcaatg aaaagaataa
     1861 gatgctggca agcatcgcga caagccggcc gtggcttgta aataatttct tcataagagt
     1921 tttagtttgt tgttttaaaa ctcttcaatg gtaagggcgg ccggtttaaa aaatgggtta
     1981 aaaggggggt taacgggtga atatatttgg ttaaccctat gaggatcatc ctactgcacg
     2041 atcttgtcga agctgacgtg ataggcgacg ccgagcgacc gttcgtagtc ctgggtgaac
     2101 tggacatagg cccggcggga gtattcgatg cctttccatt tccggtagct tttcagctgg
     2161 tatttgagca cctcttcgtt gaaggggtcc atgcccagga tcagtttggc tatttcgagt
     2221 gcccatttat agtcgccgga ttcgtatttg tttttcagtt cggggaggag ctgggtcgtc
     2281 agccgctctt cgtaggagga tttgaaatcg tccatcaggg catccggcat gtcctggagc
     2341 agggggccgc gggtcatcag ccggaaatgg tcgggaaagg tctcggggtt ctggcgtggc
     2401 ccggccagga tacagtaatc gcagaagaag ggttcggcga tggtaaagga ataattgtcg
     2461 ttctggaaga tgagttcgat gccttcgata tcgctgatga tgttccggag atgattgaag
     2521 gtgacccctt tgatgttctt tgttttggcg gggtcctttt ccggccagag tttggcggag
     2581 atcttccggg agccgatgcc ggcgcccgtg ccgccggggc tgtttgaagt cgtgccgttc
     2641 cggccggtgc tgtgcaggag gatgaggacg aagagctgtt tgatcttcgg gctgaagaga
     2701 tgggtgatat tttttccctt ccggtcgaag gcgaggaatt cgccgagcag gtagaccgca
     2761 ttccggttcc tttcgatggc ggggagtggc gaggcgggaa gcggtggggc cgcgagggcg
     2821 gcctggggtt cggctgcggg tggcgccgct ggttgctgcg cgggggtttg ggtgatcctt
     2881 ttattttttt tccgtccgaa gaaaaaccag atgagcgctg ccgatacgac gaggaagacc
     2941 gggaacaggt agaaaataaa ggccatcccc ggttttttgg tgggtcgcag ggaagcaaga
     3001 taggtggcgg tgctcaccgg gggtgatgtc agggaataaa cttttatggt ggagctgttc
     3061 cgatcgctga attcctgtac ggtgcaaaag aactcgtcgg tcttgtggct gtagaagagg
     3121 ttgacgtccg tttcgattcg cagggaggcc atggggatgg ctgcgcttac cacctcgtag
     3181 gacccgtcgc tgagcgagaa tttatacaat ttcagttctg tcgccgccag ttcgtgcgga
     3241 tagcacaggg catagaaata ttttttatcg ccggagagga tgaggttatt cgccgggacg
     3301 aagacctcgt tatggggggt tatctgccag cattttttga cggagtggtc tttcaggttg
     3361 atccggtaca gatcatagta ctgttttccg ccgaccacct ggctccccga ctcgttgcca
     3421 tagccgccga agagcaggag ctcgtcgggg ttatccgagg gaccgctggc agcgaagaac
     3481 cgcggggtta tcgtatcccc tttaaagccg gtgtgctccc agcggtcggt ggtgtcatta
     3541 taccgcagga aggcgttgtg atagctatag tgtccgtagc cgccgaagag gaagaactgg
     3601 tcctgccgcg aatcatagaa tatattatgg tgatgcagct ggtaaggcag ggcgtccttg
     3661 ccgacgatgc tccattttaa attattcctg tccatgttca gcgcggcgac ggagggggct
     3721 ccctttgcca catcgaataa ttcatagata taacatttat tttcccgggc attgaagatg
     3781 ctcttaccca ataccatgtt gacgggtatc ttgttggcat aggccgctgc catcgcccgt
     3841 ttggatgcgg ggtcataggt aaccagtgag tcctgcgtga agatgaatag atcctgatcg
     3901 aggggatcga aattcaggcc ggcgacattt ttggcggatt ggctgtatac cggtctccag
     3961 aagtaggagc cgttgatcag ccagaggggg ttctcgacgt agccggtact ctcgccgttg
     4021 ctgtcgtgaa ccgtgttacc actccattcg ttgagcggga agtgatagct ctttcgaccg
     4081 ttgccgatct ccagatcgcg gatggccata ttgggaactt cggtatagta ttgattcttc
     4141 ccaaagacga ggttggcggg catatcgtct ttgaatccca ggtgatcggc ctgccaggtg
     4201 ttgccatcga tgcggataat gaccttgtcc tccttcagca ggaaatcgat ccttaccctg
     4261 aaccagttgt tctttttgag gaaggccgcg ggcaggggaa tggagagttt attgcttttg
     4321 ctgtcgatgc tgaagttgag gttgccggcg ccgttggtat acaggtagct gaggctatag
     4381 gaattgtcct tgtcggcgag gttgaggaca taaccaagat tggcgttgtc ccagagggaa
     4441 agatcgaagg caatggagaa gcgatcgtga aagaccggcg ccggggaatt gaaaacgtga
     4501 taggaagtgc gttgcgtcag caggctgtcc tccgacttga attgcagtcc ctgggcgttg
     4561 gtgatcagga taggaaagga tagggtcaaa agcagtataa tcttggctac cttcacaagc
     4621 atgtcgttat atttcaaagg attacaaccg ttcggcgcgc cggtccggcg caggatccca
     4681 ctactaaatt agagtttttt gagtaaaatc aaacccgggg gtgacccccg tcatccgtgg
     4741 gggaacgtgt ctgggataat ttggagccca aaatcaataa atatgacttc ctatattcaa
     4801 aaattcatgg gcacgggtat ggcggacgtc gcgatcgtcg gcggcaagaa cgcttccctt
     4861 ggagagatgc tgaccgcgct atcgtcgaaa ggcatccggg tgcccgatgg ttttgcgacg
     4921 acggcggagg ctttccggca ctttctcgac cacaatgagc tacctgccag gttaggggtg
     4981 ctaatgagca gtcttgaccg gaaaagctat tccaatcttg cggaggtggg cgcggaggcg
     5041 cgaaagcttc tgtattgcgc cgatatgcct gacgacctgg gccatgcgat agcgaaggcc
     5101 tatgcggagc tgtgcgggaa tggcccggat tcggtggcgg tgcggagcag cgccacggcg
     5161 gaggatcttc cccaggcgag ctttgcgggg cagcatgagt cttttttgca tatcagcggc
     5221 gagaccgcgg tgatcgatgc cgtccagatg tgttttgctt cgttgtatac ggaccgggcg
     5281 atcaaatacc gggaggacaa tggttttgcc catgaaaagg tggctttgtc ggccggtgtg
     5341 caacggatgg tgaatgcgga caaggggtgc agcggggtct gttttacgct ggagccggaa
     5401 tcaggatttc gcgatcttat tcatatagcg ggggtatggg gtctgggaga gaatatcgtg
     5461 cagggcgaag tgacccccga tgaatttctt gttttcaaac ccttactggg aaaagtgaag
     5521 aacccggtga tccgtcgaac gctgggagcg aagcaaatga tgatgatcca tggtacagcg
     5581 aatacgggaa cccggcacac cgtgaatatc gaaacgcctg tggccctgcg tgaccgtttt
     5641 gtgctggagg acgatgagct tatgcagctg gcccaatggg cattgctgat cgagggtcac
     5701 tatggcaagc ccatggatat cgaatgggta aaggacggga acacgggcga gctttttatc
     5761 ctgcaggcgc ggccggagac ggtgcagtcg agacggaacc cttacctgct gacggagtat
     5821 gccgtatcgg ccaagggcgc ctgcctggcg aagggcgagg cggtagggaa gaatatcatt
     5881 tcgggcaagg cgagggtgct ggatagtccc gcggaggcag gacaattgca ggggggagaa
     5941 gtcatcgtca ccgatatgac cagtcccgac tgggatcctg tgttgaagaa ggcggccgcc
     6001 atcgtgacga acaagggtgg aaggacgagt catgcgtcga tcgtggcgcg tgagctgggg
     6061 gtgccggcgg ttgtgggaac gggcgatgcc acctggaaga tcaaggatgg ggatttgatc
     6121 acggtctcct gttgcgaagg aggcacgggg tttatctatg aaggaaaggc tgagtggaag
     6181 gaggctacct ccgacctgcg aaagaataca ctgccggaaa cggtaaagcc gatgctgatc
     6241 gtcggtgatc ccgacaaggc gttcgaactt tctttttatc cggctgccgg cgtggggctg
     6301 atgcggatgg aattcatcat taccaatttt atcggggtac atcccatggc gctggcgcat
     6361 ttcgacacgc tgaaggcggg tcctgtaaag gagaagatct cggcgctcac gcatgggtat
     6421 gcggataagt cacgcttttt tatcgaccgg ctgtctgagg gaattgcgat gatcgcggcc
     6481 gctttttatc ccagggaggt gatcctgcgg accagtgatt tcaagacgaa cgaatatgcg
     6541 gggctgttgg gcggcacggg tttcgagccc ggggaagaga acccgatgtt ggggttccgg
     6601 ggcgcgtcgc ggtattatag cccgctatac aaagacgggt tccggttgga atgcgcggcc
     6661 atccaaagag tccgggagga catggggctt accaacctga aggtgatgat cccgttctgc
     6721 cggacggtgg aggaaggtga gaaggtggtc gccctgatgg gagagtacgg tctgaagcgg
     6781 ggagcggacg gactggagat ctacgtaatg gcggagatcc cgagcaatgt catcctggcg
     6841 gaggaattc
//
LOCUS       DATGFG010000114         3770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1473249, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000114 DATGFG010000000
VERSION     DATGFG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473249"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VKQ52_02145"
     CDS             complement(<1..393)
                     /locus_tag="VKQ52_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86006.1"
                     /translation="MHFKTFLLAGLCLAAGLSSRATATIANLAALRSYTYTPGNESVF
                     VQGYATVDDGGQGFFAWDAASGQADNDGTVIQVGSTSGRWMRIYSGAMSVKWFGAKGD
                     SVTNDQPAISHALNAALNFQTDLFLPGGI"
     gene            535..1275
                     /locus_tag="VKQ52_02150"
     CDS             535..1275
                     /locus_tag="VKQ52_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ86007.1"
                     /translation="MSTLDQKVVLVTGASGGIGAGISRQIAKAGAKVIVHYAGRKEPA
                     DKVVEAVKAAGSDAIAIQADVRNAGEIKQLFTAAIAKFGRIDAVVNNAGIMITKFIKD
                     FTDEEFIDQMNINVTGAFFVLREAMAKVADNGVILNISTSVNRIPVPTYGPYGATKSA
                     IEHLTKVAAKEVGRGIRVNAVSPGPVNTELYTKGKTPEMIARQAALSPWNRVGEVDDI
                     APVIVFMLSDDAHWISGQIIPVNGAMVS"
     gene            1331..2083
                     /locus_tag="VKQ52_02155"
     CDS             1331..2083
                     /locus_tag="VKQ52_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ86008.1"
                     /translation="MNTLKGKVALITGSARGLGKAIAERYAALGADIVLNYSRDRIAA
                     EETLSNIKAMGAKAIAIQGDVSKVGDIERLFDEAIAAFGKIDIVVSNAGIEMVETPVT
                     KFTEEQFDRVFSINAKGSYFTMQQAAKHVTDKGRIIYIASSTTSFPVPGMAVYGGSKT
                     TPRYLVDVLSKEIGHRGVTVNSIIPFAVDHAGIFVDPKAYPELRKQLLDSCPMGRLAE
                     VEDVANAAEFFASDLSSFISGQHLLVNGGATN"
     gene            complement(2244..2729)
                     /locus_tag="VKQ52_02160"
     CDS             complement(2244..2729)
                     /locus_tag="VKQ52_02160"
                     /inference="COORDINATES: protein motif:HMM:NF025124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ86009.1"
                     /translation="MFLRVYDDKLELWNPGRLPEELTIEKLKERHSSYPRNPHIAEVF
                     FKAGYIKAWGRGTTNIINECLTAGLPEPIFEEDQGGVRITFLKDIYTKDFLDQLGINS
                     RQIQAILFVKQNGYITNRQYQLEYQVSDRTALRDLDNLVTRDILVKTGDKKGSRYLLK
                     K"
     gene            2832..>3770
                     /locus_tag="VKQ52_02165"
     CDS             2832..>3770
                     /locus_tag="VKQ52_02165"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HLZ86010.1"
                     /translation="MNQRYYSLDVFRGATVAFMILVNNQAGASYTPLDHAPWHGLTPT
                     DLVFPFFLFAVGNALAFVMPRFEEKGNAYFLQKVFRRTLLIFVINVLLNWFPFVHWSH
                     DHLTLTGWTWTNDNGNLAGVRVMGVLARIALAYCGAALIVYYAKLRGAFFISGCLLIA
                     YWALCLFLGDPADPYSLNGYFGTAIDKAVFGPEHMYHGEGVAFDPEGLASTIPAIVSV
                     ILGYFAGTYIRLKGKTYEMLSHLLVAAFILLFAGYCWGSIFPINKKIWTSSYVFVAAG
                     WALATLSLLIYFIEFRNSTGSWSKFFDVFGKNPLFIF"
BASE COUNT          872 a         1206 c          870 g          822 t
ORIGIN      
        1 gatcccacca ggcaggaata aatcggtttg gaaattcagg gctgcattca gcgcatggga
       61 aattgccggc tggtcattgg tcacgctatc gcctttcgca ccgaaccatt tcacggacat
      121 cgctccgcta tagatgcgca tccatctccc ggatgtcgac cccacctgta tcacggttcc
      181 atcgttgtcg gcctggccgc tggcagcatc ccaggcaaaa aacccctgtc cgccatcgtc
      241 gacggtggca taaccctgaa caaagacgga ttcattcccg ggggtgtacg tataactccg
      301 taaggcggcg agattggcga tcgtggcggt tgcccgggaa gaaaggccgg cggcaagaca
      361 aagcccggcc aggaggaagg ttttgaagtg catagtataa ggttgttgtt gactcaattt
      421 attaaatttt cggtaaaccg gttctttaaa ttatataact gatcctataa ttcccgtcct
      481 tcccccggcc cgtccccata gtagctttgt cttatcaaac aaaaacaaag aactatgagc
      541 acgctagatc aaaaagtagt actggtcacc ggcgcctcgg gcggcatcgg tgccgggatc
      601 tcccgccaga tcgcaaaggc cggggcaaag gtgatcgttc attatgcagg tcgtaaggaa
      661 cccgccgata aagtagtcga agccgtaaaa gccgccggca gcgacgccat cgccatccag
      721 gccgacgttc gcaacgccgg tgagataaaa cagctcttca cggccgcgat agccaaattc
      781 ggccgcatag acgccgtcgt taacaacgcc gggatcatga tcacaaaatt cataaaggac
      841 ttcaccgatg aagaattcat cgatcaaatg aacatcaacg ttacgggcgc tttcttcgtg
      901 cttcgcgaag ccatggcgaa agtggccgac aacggcgtca tcctcaacat ctcgacaagc
      961 gtcaaccgca ttcccgttcc cacctacggt ccctatggcg cgacaaaatc cgccatcgaa
     1021 cacctgacaa aggtggccgc caaagaagta ggccgcggca ttcgcgtcaa cgccgtctcg
     1081 cccggccccg tcaataccga attgtataca aaaggcaaga cccccgaaat gatcgcccgc
     1141 caggccgctc tctcgccctg gaatcgggta ggggaggtcg acgacatcgc acccgtcatc
     1201 gtcttcatgc tctccgatga cgcacactgg atcagcggcc agatcatccc ggtcaatggc
     1261 gcaatggtat cataagaaca gtcaatcctt ctatcaacaa caatctgcaa caacaataaa
     1321 aattacgatc atgaacacct tgaaaggaaa agttgccctt atcacaggat cagcaagagg
     1381 ccttggcaaa gccatcgccg aacggtatgc cgccctcggc gccgatatcg tcctcaacta
     1441 ttcccgcgac aggatcgccg ccgaggagac cctcagcaat ataaaagcca tgggcgcaaa
     1501 ggccatcgcc atccagggcg acgtcagtaa ggtcggggac atcgaaaggc tcttcgacga
     1561 ggcgatcgcc gcctttggta aaatcgacat cgtcgtctcc aatgccggta tcgaaatggt
     1621 cgaaaccccc gtgacgaagt ttacggaaga acaattcgac cgggtcttct ccatcaacgc
     1681 aaagggctcc tatttcacga tgcaacaggc cgccaaacac gtcaccgaca aaggccggat
     1741 catctacatc gcttccagca ccacctcctt ccccgtaccc ggtatggccg tctatggcgg
     1801 cagcaaaacg acaccccgct acctcgtaga cgttctctcc aaggagatcg gtcaccgcgg
     1861 cgtcaccgtc aactccatca tccccttcgc ggtcgaccat gcgggtatct ttgtcgatcc
     1921 caaagcctac cccgaactcc gcaagcaact gctcgactcg tgtcccatgg gccgcctcgc
     1981 cgaggtggaa gacgtcgcca acgccgccga attcttcgcc agcgacctct cctccttcat
     2041 ctccggccag cacctcctcg tcaacggcgg cgccaccaac taacccctct ccaactgatc
     2101 ccgcgccaac taacgccgca ccaactaacc cggccccaac tgatcctacc cctcaccagc
     2161 cccccaaacc aactgcccca cagcccaacg cgatcccggc ccattgccgg gatcgccaca
     2221 tatccgccac atatccgcca tgatcatttc ttcaataagt aacgactgcc ttttttgtcg
     2281 cctgttttaa ccaggatatc ccgcgtaacg aggttatcca ggtctctcag cgcggttcgg
     2341 tcagatacct ggtattccag ttgatactgg cgattggtaa tataaccatt ttgcttgacg
     2401 aaaaggatgg cttgtatctg ccggctattg atcccaagtt gatccaaaaa atcttttgta
     2461 taaatgtctt tcagaaaggt tatcctgacg cctccttggt cttcctcaaa tatcggctct
     2521 ggtagaccgg cggtcaggca ttcgttgatt atattagtag tcccccttcc ccaggctttg
     2581 atatagccgg ctttgaagaa tacttccgca atatggggat ttcggggata ggaggaatgc
     2641 cgttctttga gtttttcaat ggtgagctcc tccggtagcc ggccggggtt ccataattcc
     2701 aatttatcgt cgtatactct caggaatatc catgatttaa agtgagcaaa ggtaggattt
     2761 tggcaaggtc tttaaggtcg caaaacgcga ccttaaagat gcagtccgga gaatttcttt
     2821 atcttcaacc gatgaaccaa cgctactact ccctggacgt cttccgtggc gctaccgtcg
     2881 ccttcatgat cctcgtcaac aaccaggcgg gcgcctccta caccccgctc gaccacgcgc
     2941 cctggcacgg cctgaccccc accgacctcg tcttcccctt cttcctcttc gccgttggta
     3001 acgccctcgc cttcgtcatg cctcgcttcg aagaaaaagg caacgcttac ttcctccaaa
     3061 aggtcttccg ccgcaccctc ctgatctttg tgatcaacgt cctgctcaac tggttcccct
     3121 tcgtccactg gagccacgac cacctcaccc tcaccggctg gacctggacg aacgacaacg
     3181 gcaacctcgc cggcgtccgg gtcatgggcg tccttgcacg catcgccctg gcctactgcg
     3241 gcgccgccct catcgtctac tacgcaaaac tcaggggcgc cttctttatc agcggctgtc
     3301 tcctcatcgc ctactgggcc ctctgcctgt tcctgggcga tcccgccgat ccctatagcc
     3361 tcaatggcta cttcggcacc gccatcgaca aagccgtctt cggtcccgaa catatgtacc
     3421 acggggaagg cgtcgccttc gaccccgaag gtctcgcgag taccataccc gcgatcgtca
     3481 gcgtgatcct gggatatttt gccggaacct atatccgact gaaagggaaa acatatgaaa
     3541 tgctttccca tcttctcgta gcggccttta tcctcctttt cgccggctat tgctggggca
     3601 gcatcttccc catcaacaaa aagatctgga caagcagcta tgtattcgtc gccgccggct
     3661 gggcgctggc gaccctgtcc ctcctcatct acttcatcga atttcgcaat agcaccggtt
     3721 cctggagcaa attcttcgac gtcttcggca agaaccccct ctttatcttc
//
LOCUS       DATGFG010000115         4940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_891032, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000115 DATGFG010000000
VERSION     DATGFG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891032"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2998
                     /gene="secDF"
                     /locus_tag="VKQ52_02170"
     CDS             <1..2998
                     /gene="secDF"
                     /locus_tag="VKQ52_02170"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HLZ86011.1"
                     /translation="ESAMETKAKTQVSRLFPQSAAQKYPGNKEEQAAYQDSIDQAFNA
                     RQKKLLDSTKDIKITPWGTTYQKAKESELLLGLDLQGGISVTMDVALDGLIKGLANNS
                     RDVRLVQALNLAIQKKQTQSGNLIDLFAGSYKEINPTGKLAPLFSNSNRNKISYDASD
                     ATVISYLHEQAIAAMKQTYQILDNRINQFGVAQPSINLDETKGIITVELAGATDPDRV
                     RKYLQSSANLQFWEVYNLGELSQSIQNADKTLQNYLNGVSNVSKADTTTGHTDSTKTA
                     KADSAKSKDTANQNPLMRLVHFLEPTQDQKTGQPHFYPQLGVARLQDTGLISSYLNNP
                     IVRNQFPQDLKFLWGKQNADEDGKLSNVLDLYAIKTVPGTDKAKIEGSIIENATQDFD
                     PTTGGVVVEMSMNKQAAKVWADMTTKNVSKPIAIVLDNIVYSAPNVSEPITGGQSRIT
                     MGSGRNSNQTITEAQDLANILKSGKLDAPARIVSETVVGPTLGKAAVNGGMMAFGISF
                     LVIFTLMLVYYNSAGWVANCALILNLLFTIGVLSALHATLTAPGIAGLVLTVGMAVDT
                     NVIIFERIKEELTRGKSYPIAVKDGYRRSLAPVLDAHVTTLLTALILFYFGLGPVLGF
                     ATTQILGILLSLFCGILVSRLITDFYTNKNRHFNYFTPLSRRIFKHASFKFIEYRKVA
                     YGISVVVLILGVGSFFNGFREGVEFQGGRSYIVNFHKPVQQEEVRVALQKTLTGTNPE
                     IKTYGSTEQLDITTDYKVKETGSAADSSVLFTTFQGLKPFLPAGLSYQEFTSSKYLQG
                     NVKVEPSISEELKSGAVKATIFALIVIFLYIFIRFRDWRYSLGTIIALLHDVFVTLAV
                     FSFLKSVVPFTLEIDQHFIAAVLTVIGFSMNDTVIVFDRIREDSKLMKGATNGEIINK
                     AVNDTLSRTIMTSLTVFLTLLILFLVGGEVTRGFAFAMLIGVITGTYSSIFVAAPFLV
                     DFAKGRPLGEAKEAAKPAVKTKKVTA"
     gene            complement(3283..3645)
                     /locus_tag="VKQ52_02175"
     CDS             complement(3283..3645)
                     /locus_tag="VKQ52_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86012.1"
                     /translation="MKKILLIVNLLFAFAGFTFAQTTPPKTKDAKTKPTTTATTKPAT
                     TATTKTPTTAASTTKPATTAAGPTKKDGTPDMRYKANKDAAAKPAGPTKKDGTPDMRY
                     KANKDAAKDKDAKSKKTN"
     gene            3852..>4940
                     /locus_tag="VKQ52_02180"
     CDS             3852..>4940
                     /locus_tag="VKQ52_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ86013.1"
                     /translation="MRSSTLKWIVLSATLLAAIVVTVQLYWLKKVYSFEQRQFNLNVV
                     KSIRGLFEDLEMNDAPGTSLQQLIDAPENNYFSFKADTIPPKDSLVFYIQDEFVDFGV
                     LTDCKLGAYSAADKKFVYEEFIPAPASRYAGVKPVTQELPVYDNAKGYDHILLYFPHR
                     QEYILEQMDFWIVSTVVLFLALIGLAASLFYFYRQKFLTEIQKDFVNNFTHEFKTPLA
                     VMKIAADVLSQESIVHQPDRLERYSNIIRNQTEHLQGQVERLLRTASIESKDLPIRKE
                     DVDPQLLIEQALGKVQPLIEQKKAKIEVKMEEVVGNGRVHADKAHLELAVVNLLENAL
                     KYSNEPHIVVETGQLEDDFYISVKDNGVG"
BASE COUNT         1181 a         1422 c         1275 g         1062 t
ORIGIN      
        1 tgaatccgcg atggagacaa aggcgaaaac acaggtgtcg aggctgttcc cgcagtcggc
       61 tgctcaaaag tatcccggca ataaagagga gcaggcggcc taccaggata gcatcgacca
      121 ggcttttaac gcccggcaga agaaattgtt ggatagcacc aaggatatca agatcacccc
      181 ctggggaacc acctatcaga aagcaaagga aagcgagtta ctgctcggcc tcgatctgca
      241 gggcggtatc agcgttacca tggacgtcgc cctggacgga ctcatcaagg gtctggccaa
      301 caactcccgc gacgtccggc tggtgcaggc gctgaacctc gccatccaga agaaacagac
      361 ccaaagcggc aacctcatcg atctgtttgc cgggtcgtat aaggagatca accccaccgg
      421 taagctcgct ccgctgttct ccaacagcaa ccgcaataag ataagctatg acgcttccga
      481 tgccaccgtc atcagctacc tgcatgagca ggccatcgcc gccatgaagc agacctacca
      541 gatcctcgac aatcgtatca accagttcgg ggttgcgcag ccaagcatca acctcgatga
      601 aacgaaaggc atcatcaccg tggaactggc cggcgccacc gaccccgacc gcgttcgcaa
      661 atacctgcag tccagtgcca acctccaatt ctgggaagta tataatcttg gcgaactgtc
      721 gcaatccatc cagaatgccg acaagaccct gcagaactat ctcaacgggg tcagcaatgt
      781 ctcaaaggcc gataccacca ctggccatac cgactcgaca aagacggcga aggcggactc
      841 cgcaaagagc aaggatacag ccaatcagaa cccgctgatg cgcctggtgc atttcctgga
      901 acccacacag gaccagaaga cgggtcagcc gcatttttat ccgcagctgg gagtagcccg
      961 cctgcaggac accggcctga tcagcagcta cctgaacaac ccgatcgtcc gcaaccagtt
     1021 cccgcaggac ctcaaattcc tctggggcaa acagaacgcg gatgaggacg gcaagctcag
     1081 caatgtactc gacctgtatg cgataaagac cgtgcccggc accgataagg caaagatcga
     1141 agggtccatc atcgagaacg ccacccagga cttcgatccc accaccggtg gtgtagtggt
     1201 cgagatgagc atgaacaaac aggcagcgaa ggtctgggcc gatatgacca ccaagaatgt
     1261 cagcaagccc atcgccatcg tcctcgataa tatcgtttat tccgctccta atgtaagcga
     1321 accgatcacc ggcggccagt cccgcatcac catgggcagc ggccgcaact ccaatcaaac
     1381 catcaccgag gcgcaggacc ttgccaacat cctgaagtcg ggtaagctgg atgcccccgc
     1441 caggatcgta tcggaaacgg ttgtcggtcc caccctgggt aaggcagccg tcaacggcgg
     1501 catgatggcc ttcggcatct ctttcctcgt catcttcacg ctgatgctgg tgtattacaa
     1561 ttcggcggga tgggtggcca actgcgccct gatcctcaac ctgctgttca ccatcggcgt
     1621 cctcagcgcc ctgcacgcca ccctgacggc tcccggtatc gccggtctcg tcctcacggt
     1681 gggtatggcc gtcgacacca acgttatcat cttcgaacgt ataaaggaag agctcacgcg
     1741 cggcaagagc tacccgatag cggtgaagga tggctacaga cgatcgctgg cccctgtcct
     1801 tgatgcgcac gtcacaactc tcttgaccgc cctgatcctg ttctactttg gcctcgggcc
     1861 ggtacttggc ttcgccacca cgcaaatact gggtattttg ctctctttat tctgcggaat
     1921 tctggtatcc cggcttatca ccgacttcta taccaacaag aacaggcact tcaactactt
     1981 caccccgctg tcccgccgca ttttcaagca tgcttccttc aagttcatcg agtaccggaa
     2041 agtggcgtac ggcatttccg tcgtcgtcct gatcctgggt gtcggctcct tcttcaacgg
     2101 attcagggaa ggcgtcgaat tccagggcgg ccgcagctat atcgtcaact tccacaaacc
     2161 ggtacaacag gaagaagtgc gggttgccct tcagaagacc ctcaccggca ccaatcccga
     2221 gatcaagacc tatggtagta ccgagcagct ggatatcacc accgactata aggtcaagga
     2281 aaccggttcg gcggccgact ccagcgtgct gttcacgacc ttccagggcc tgaagccctt
     2341 cctgcccgcg ggtctcagct accaggaatt cacttcctcc aaatacctgc agggcaacgt
     2401 aaaggttgag ccgagcattt cagaggaact gaagtcgggc gcggtgaagg ccaccatctt
     2461 tgcactgatc gttatcttcc tgtatatctt catccgattc cgcgactggc gctattccct
     2521 gggaacgatc atcgcgctgc tgcacgacgt attcgtcacg ctggcagtgt tctccttcct
     2581 gaaaagcgta gtacccttca cgctggagat cgaccagcac tttatcgcgg ccgtgctgac
     2641 cgttatcggt ttctcgatga atgacacggt tatcgtattc gaccggatcc gcgaggacag
     2701 taagctgatg aagggcgcca ccaatggcga gatcatcaac aaggcggtga acgatacgct
     2761 gagccgtacg atcatgacct cgctgacggt gttcctcacc ctccttatcc tcttcctcgt
     2821 tggtggagag gtcacccgcg gctttgcctt tgcgatgctg atcggggtga tcaccggaac
     2881 ctattcctcc atcttcgttg cggcgccgtt cctcgtagac tttgccaagg gccgtcccct
     2941 gggtgaagcg aaggaagccg caaaaccggc cgtcaagaca aaaaaagtaa ccgcctgata
     3001 aacggcggcc acaaataaag aacacaaaaa acccggaggt gctctccggg ttttttattt
     3061 attcactaat gatgtgcggg tcgatgcgca gactggtgcg aatgatgcac cacgtgatgc
     3121 cgttgatgat ggaccacgcg gcggtggtga acaggatgat gcgattgtgc catggaaaaa
     3181 cttgtcatac cgataaatac tactgcagcc aataagaatt tcttcatgtt gttgatttta
     3241 acaggcggaa aatagcccta acaatattag caaaaaaagt gcctagtttg tcttcttgga
     3301 tttagcatcc ttatccttgg cggcatcctt gttggcctta tagcgcatgt cgggagtgcc
     3361 atccttcttc gtcggccctg ccggctttgc ggcagcgtcc ttatttgcct tgtaccgcat
     3421 gtcgggggtg ccgtcctttt tcgtgggacc ggctgcagtg gtcgccggct tcgtggtaga
     3481 ggcggccgtc gtcggggtct tcgtcgtagc ggtcgttgcg ggcttggtcg tagccgtcgt
     3541 cgtgggcttg gtcttggcat cctttgtctt cggaggcgtg gtctgggcga aagtaaatcc
     3601 ggcaaaggca aacaacaaat tgacaatcaa gagaatcttt ttcataaacc gggtgttttc
     3661 tggtgaaaga atagagaaat acgtggtgaa tttattcaaa tgaatcggtg taacgaaaaa
     3721 aaatcctcgt ttattgctgt aggaacaggc agttgtatat tagaaagata gccgatattt
     3781 ggctatggcc ttaaccacta cttaactatt ttcctggtat actaagccca aagctcagca
     3841 attttgcatc catgcgttca tcaaccttaa aatggatcgt cctttccgcc accctgctcg
     3901 cagcgatcgt ggtgaccgtc cagttatact ggttgaaaaa agtctattcg ttcgaacaac
     3961 gacagttcaa tctcaacgtg gtaaagagca tccgggggct tttcgaggac ctggaaatga
     4021 acgacgctcc gggaacgagc ctccagcagc tgatcgacgc cccggagaac aattatttct
     4081 ccttcaaggc ggataccatc ccgcccaagg actccctggt gttctacata caggacgagt
     4141 tcgtggactt tggggtattg accgattgca agctgggcgc ctacagcgcc gcggacaaga
     4201 agttcgtgta cgaggaattc atccccgccc cggcctcccg ctatgccggg gtcaaacccg
     4261 tcacccagga gctgccggtg tacgacaatg caaagggata tgaccatatt ctcctgtatt
     4321 tccctcaccg gcaagagtat atcctggaac agatggactt ctggatcgtc agtaccgtcg
     4381 tgctctttct tgcccttatc gggctggccg ccagtctctt ttatttctat cgccagaaat
     4441 tcctcacgga gatccagaag gacttcgtga acaattttac ccatgagttc aagacaccgc
     4501 tggccgtgat gaagatcgcc gcggacgtcc tttcccagga gtcgatcgtc caccagcccg
     4561 accggctcga acggtattcc aatatcatcc gaaatcaaac ggaacacctt cagggccagg
     4621 tggaacgcct cctccggacg gcctccatcg aatccaagga cctgcccatc cgcaaggaag
     4681 atgtcgatcc gcagctgctg atcgaacagg cccttggaaa ggtgcagccg ctgatcgagc
     4741 aaaagaaggc gaagatcgag gtgaagatgg aagaagtggt cggtaatggc cgggtacatg
     4801 ctgataaggc ccatctcgaa ctggccgtgg tcaacctcct ggagaacgcc ctcaaatact
     4861 cgaacgagcc ccatatcgtg gtcgagacgg gacaactgga agacgacttc tatatctccg
     4921 tcaaggacaa tggggtcggc
//
LOCUS       DATGFG010000116         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_776203, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000116 DATGFG010000000
VERSION     DATGFG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776203"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1069
                     /locus_tag="VKQ52_02185"
     CDS             182..1069
                     /locus_tag="VKQ52_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HLZ86014.1"
                     /translation="MAIASMPAEKATPPTTAKPITVDRRSKLSYQEFKNEYLIPRKPV
                     IITDATAAWKASAWTPQFFRERYPNKMLTTDQGEMRMEDFIEAITNNSDKPGPFLREQ
                     PLKDVFPDLVDHVQPTPPYVQPNWLSGKYLVGSVTRRLNRESYIEVNFCGRRIFPYLH
                     IDDLGVHAFITQHYGDKEIVIYPPDQEPFLYRKPGSRFSEIVDVDHPDLEKYPLFAKA
                     TATRVVVKAGESAFMPSGWWHTTRVPGTSLSTVISLSNSSNWPDLVDYLQSNSYRPRL
                     FSFYAMYLRSVGFIKSRLS"
     gene            1157..2794
                     /locus_tag="VKQ52_02190"
     CDS             1157..2794
                     /locus_tag="VKQ52_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86015.1"
                     /translation="MPPITGTSPLQKGYKKFYIIGCSLLLLLAFIQCYSAASGIHWFF
                     DPDYYRDMSCVQQNLYGNFGKDPNYLGAYLWYNPLLTSLETLIVKITGLPLNLAFAKA
                     GIYLNLLAPVTFTLMLATLFDWRIAAAGLLSYLFLSSGNHYTFDAATYSPWLYPGSFT
                     QSIFYLNIIFCYKAFTTQKYPCFLALGAMLGISFLGHTAPTLLIILILVILQTGNLIK
                     SFREKDYAAVRRYIGQGAIVAGLFIVFAMPLLYYIVGKYRLHFINRMPFEFVEPLFIF
                     RNLPTLLRMNFSIAALIALTGVVWAVFNIKQPVIRKIIFAWPLLAIFLFVYSTVVSSL
                     DRNYNIHLPGTVPAFHYFFYLKAAQSVFYGFGLVFLLQLLFAKLRSLQVPRTREAFFI
                     ITILLCAIVYFPFYRNRQDFVFYRTRNLQMDKDTDRISTYNYILKNVPSDKVILCEEN
                     TSIFPVMATARKMVSSGITYSNPYVDFWQRENARNSMLSYLKTGQPTAAKQLFDTYQV
                     NFILLSNAVFGDPATLSGLDSRLVYKNDSYSLYCIKM"
     gene            2820..>3941
                     /locus_tag="VKQ52_02195"
     CDS             2820..>3941
                     /locus_tag="VKQ52_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLZ86016.1"
                     /translation="MTTPNRRYWLHSGFLSLMINFQNLLFGFGSFYFLVRELDKHHYG
                     IWSLFIATTTIFDSARSGMVQNALIKYLSDHSKKEHPAILTASFYLSGGLMLLCMIAN
                     IGLAGYLAHIWHDQGLINLFYAFNIVYLLQGILYQFQWIEQANLSFKGVLITSIIRQG
                     GFFTYVVLAYFLRWPLSLISLVWVQAGCAFVAMLIQYLFVADELVLAKKPDLAWVKKL
                     FNYGKYVFGTNISGILTGSINQMMLGAIVSADTAGAFNVAVRITTLTEVPTNALGTIV
                     FPQSAKRFAADGKDAIKYLYEKSVGTLLAILIPSLGFLFLFPGFVVHVIAGNQYPETI
                     PVIRLTVLYCLLTPFTRLFGTIMDSIGKPRLNFIIIVLFT"
BASE COUNT          903 a         1324 c          764 g          950 t
ORIGIN      
        1 tggcgaggat caccagtttc tcatgcaaac tccgttccct gttagtggta ttttcattct
       61 tgctcataaa cgaacccgtt cattatcaat aatgcggtcg gaaaaatacc ggattacccc
      121 ggtaaaaagg tataaaactt cataaaatta tgtatatctt ggcaataatt ataacgtgat
      181 tatggcaatc gcaagtatgc ctgctgaaaa ggcaacaccc cctaccaccg ccaaaccgat
      241 caccgttgac cggcgaagca agctctctta ccaggaattc aaaaatgaat acctgatacc
      301 caggaaaccc gttatcataa ccgatgccac tgccgcctgg aaagcctccg cctggactcc
      361 ccaattcttc agggaaagat atccgaataa gatgctcacg accgatcagg gcgaaatgag
      421 aatggaagac ttcatcgaag caatcacaaa taatagcgac aaaccgggtc ccttccttcg
      481 cgaacagccc ctgaaagacg tcttccccga cctggtggac catgtacagc cgactccgcc
      541 ctatgtccaa ccgaactggc tgagcggtaa gtatctcgtc ggctccgtta cccgccgcct
      601 caaccgcgag tcgtatatcg aagtaaattt ctgcggtcgc agaattttcc catacctgca
      661 catcgacgac ctgggcgtcc acgcctttat cacccagcac tacggtgaca aagagatcgt
      721 catctatccg ccggaccagg aacccttcct ctaccggaaa cccggttccc gtttctcgga
      781 aatcgtcgac gtcgaccatc cggacctcga aaaatatcca ctcttcgcaa aagccaccgc
      841 cacccgcgtc gtcgtaaaag caggtgaaag cgccttcatg ccatcaggct ggtggcatac
      901 cacccgcgta cccggcactt cactgagcac cgtcatcagc ctgtccaatt cctccaactg
      961 gccggatctc gtagactatt tgcaaagtaa tagctaccgg ccccgccttt tttccttcta
     1021 tgctatgtac ctccgcagcg tcggatttat aaaatcccgg ctgagctgac ccgctgatcc
     1081 tcgcccgcca accatccaaa atcaatatcc tgcgatcaga accgcggtat tcgattttgt
     1141 atttaaacta ccccccatgc cccccatcac cgggaccagt cctttgcaaa aaggatataa
     1201 aaaattttat atcatcggct gctccctgct gctcctcctc gcattcattc aatgctattc
     1261 cgcggcatcc ggcatacatt ggttcttcga cccggactac taccgcgata tgtcctgcgt
     1321 ccagcaaaat ctctacggca attttggcaa agatcccaac tacctcggcg cctatctctg
     1381 gtacaatccc ctgctcacaa gtctcgagac cctcatcgta aagatcacgg gactgcccct
     1441 gaacctggcc tttgcgaaag ccggcatcta cctcaacctc ctcgcacccg tgaccttcac
     1501 cctcatgctg gccaccctct tcgattggcg catcgccgcc gccggcttac tgtcctatct
     1561 cttcctttcc tccggcaacc actatacctt cgacgcagcc acctattccc cctggctcta
     1621 ccctgggtcc tttacccaat ccattttcta tttgaatatt atcttctgct acaaagcctt
     1681 taccactcaa aaatatccct gtttcctggc cctcggcgcc atgctcggca tcagcttcct
     1741 cggccacacc gcgccgaccc tgctgatcat cctcatcctc gtgatcctcc agacaggcaa
     1801 tctcataaaa agcttccgcg aaaaagacta tgcagccgta agaagataca tcgggcaggg
     1861 tgccatcgtt gccgggctgt tcatcgtctt cgccatgccc ctgctgtatt atatcgtcgg
     1921 caaataccgc ctccatttta tcaatcgcat gcccttcgag ttcgttgagc ccctgttcat
     1981 cttccgtaac ctccccaccc tgctccggat gaacttctcc atcgccgccc tcatcgccct
     2041 caccggcgtg gtctgggccg ttttcaacat aaaacaaccc gtgatccgca agatcatctt
     2101 cgcctggccg ctgctcgcca tttttctgtt cgtctattcc actgtcgtca gctccctcga
     2161 caggaactat aatatccacc tgcccggcac cgtccccgcc ttccactatt ttttctacct
     2221 gaaagccgcc cagtccgttt tctacggctt tggccttgtc ttcctcctgc agctcctctt
     2281 cgccaaattg cgttccctgc aggtacccag gacacgggaa gccttcttca tcatcaccat
     2341 cctgctctgc gcgatcgtct acttcccctt ctaccgcaac cggcaggatt ttgtcttcta
     2401 ccggacccgg aacctgcaga tggataaaga caccgaccgg atcagtacct ataactacat
     2461 cctgaagaac gtcccgtcgg ataaagtgat cctctgcgaa gagaacacca gcatcttccc
     2521 cgtgatggcc accgcaagaa aaatggtctc cagcgggatc acctattcca acccctatgt
     2581 ggatttctgg cagcgcgaaa atgcgcgcaa tagtatgttg tcttacctga agaccggaca
     2641 acccaccgcc gcaaaacaat tattcgatac ctaccaggta aacttcatcc tgttatcaaa
     2701 cgccgtattt ggtgatccag cgacactttc cggtcttgac agcaggctcg tctataaaaa
     2761 tgattcgtac tcgctatatt gcataaaaat gtagcccttc ggaaacgcag gctgacacca
     2821 tgactactcc taatcgccgt tattggcttc attccgggtt cctgagcctg atgatcaatt
     2881 tccaaaatct gctattcggc tttggaagtt tctatttcct cgtccgcgaa ctcgataagc
     2941 atcactatgg catctggtcc ctcttcatcg ctaccacgac gatcttcgac tctgcccgca
     3001 gcggcatggt ccagaatgcc ctcatcaaat acctgtccga ccattccaaa aaagaacacc
     3061 cggccatcct caccgcttcc ttctacctga gcggcggact catgctgctc tgcatgatcg
     3121 ccaacatcgg cctcgcaggc tacctggccc atatctggca cgaccaggga ctgatcaacc
     3181 tgttctacgc cttcaatatc gtctacctcc tgcagggcat cctctaccag ttccagtgga
     3241 tcgaacaggc caatctcagc ttcaagggcg tactcatcac cagcatcatc cgccagggcg
     3301 gcttcttcac ctacgtcgtg ctggcctact tcctccgctg gcccctatcc ctgatcagcc
     3361 tcgtctgggt acaagccggc tgcgcattcg tagccatgct catccaatac ctcttcgtcg
     3421 ccgacgaact ggtcctcgcc aaaaaaccgg acctcgcctg ggtaaaaaaa ctcttcaact
     3481 atggcaaata tgtattcggc acgaacatca gcggtatcct caccggctcc atcaaccaga
     3541 tgatgctcgg cgccatcgta tcggccgaca cggccggcgc atttaatgtc gccgtccgta
     3601 tcaccaccct caccgaagtc cctaccaacg ccctgggcac gatcgtgttt ccccaaagcg
     3661 ccaaacgctt tgccgccgac ggcaaagacg caataaaata cctgtacgaa aaatccgtcg
     3721 ggaccctcct cgccatcctc atcccatccc tcggcttcct gttcctcttc cccggattcg
     3781 tggtccatgt catcgcaggg aaccaatacc cggaaaccat ccccgtcatc cggctcaccg
     3841 ttctctattg cctgcttacc ccctttaccc ggctctttgg caccatcatg gattccatcg
     3901 gtaaacccag gttgaatttt atcatcatcg tcctcttcac t
//
LOCUS       DATGFG010000117         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1346514, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000117 DATGFG010000000
VERSION     DATGFG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346514"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VKQ52_02200"
     CDS             <1..895
                     /locus_tag="VKQ52_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86017.1"
                     /translation="FQTEAGVVDPATGQAALETMRELYGNIDKKLFHANPIAVAEYMS
                     TTDDYWYCPFAYGYSNYSRDGYARHRLEYADLVFFDPTTNQNQTNQISPNPTPTNPSP
                     TPPTSPAAVHDQAGVGMPWLAHRMANSNHPKSSGNRIRATDGDVPTRPSEELQPAATA
                     KPHREPRRLRSTIGGTGLSISATSRHPTECLAFAQWIVSPECQSTFYTEHGGQPGHRQ
                     AWLNEKTNRLTHNYFYTVLPAMERGYMRPRYHGYLYFQDHGGDPIREYLLGNKDCRST
                     LQCLDQLYHESLKKNPTTQTV"
     gene            892..2034
                     /locus_tag="VKQ52_02205"
     CDS             892..2034
                     /locus_tag="VKQ52_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLZ86018.1"
                     /translation="MNQLPLNGLTVLEFCQYLSGPSAGLRLADLGARVIKIERPGTGD
                     ACRHLSIKNLWVDGDSLLFHTINRNKESFTADLKSPEGLKNIKKLIGTADVLTHNFRP
                     GVMEAAGLGFEDIKALNPRLIYGAISGYGTEGPWKDRPGQDLLLQSMSGLAYTTGNEK
                     DNPMPFGLSIADSLCGAQLVQGILAALIRRGKTGQGALVEISMMESLLDFQFEFLTTY
                     HSSGQLPRRSKQSNGNPLLSAPYGIYATADGHIAIAMVNIPQLAQAVDSAALAAYSQD
                     QAFSHRDEIKGLLAQHLTAYPTKYWLERLHAEDMWAMEVLDWRTLTAQEGYKILQMEQ
                     AVTTTSGKRVITTRCPIRIDGQRLFSEKPAPALGQDNEKIVTELSK"
     gene            2039..3187
                     /locus_tag="VKQ52_02210"
     CDS             2039..3187
                     /locus_tag="VKQ52_02210"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLZ86019.1"
                     /translation="MDLLNDIIVVDFSQFLSGPSAGLRLADMGARVIKIERPGIGDIC
                     RQLYVSDTRIEGESTIFHAINRNKQSYAADLKDKHDLDKIKKLIEKADIVLHNFRPGV
                     MERIGLDYATVKAIKPDIIYGEVSGYGAEGPWKDLPGQDLLVQALSGLTWLSNNRDER
                     PTPMGVSVVDILAGTYLTQGILAALYRRGISGQSALVQVSMMEAILDFQFEVLTCHYN
                     DGRQLPVRSRVSNGNAYIPAPYGIYKTREGYLSLAMGDILKLAALLECQPLGQFSDSA
                     DWFPRRDEIKAILAQHLLLRTADEWLAILQPADIWCAKVMDYHDLLQQLGYKVLNMEL
                     KVKTSNGLSIRTTRCPIRVDGKIAVSEKGAPLLGEHNALIDAQFGLVK"
     gene            3213..>3476
                     /locus_tag="VKQ52_02215"
     CDS             3213..>3476
                     /locus_tag="VKQ52_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86020.1"
                     /translation="MKEKLVDTHIREELIDTHSKEELVDTHIHVWNFDQADYPWLKGD
                     TSILNRSYDLSGLEPQRSRAGVTSGVLVQAANNPGDTAWMVRTA"
BASE COUNT          853 a         1210 c          836 g          577 t
ORIGIN      
        1 cttccagacg gaagcaggcg tcgtcgaccc cgccaccggc caggccgccc tcgaaacgat
       61 gcgcgaactc tacggcaaca tcgacaaaaa actattccac gcaaacccga tcgccgtcgc
      121 cgaatatatg agcactaccg atgactactg gtactgcccc ttcgcctacg gctattccaa
      181 ctattcccgc gacggctatg cccgccatcg cctcgaatat gcggatcttg tcttcttcga
      241 tccaacaact aaccaaaacc aaactaacca aatctcacct aatccaaccc caactaaccc
      301 atccccaact cccccaacat cccccgccgc tgtccacgac caggccggtg tcgggatgcc
      361 ctggctggcg catcggatgg ctaactccaa ccatccaaaa agctcaggta acagaatccg
      421 cgcgacagac ggggacgtcc cgacccggcc cagcgaagaa ctccaacccg ccgcaacagc
      481 aaaaccccac cgggaacctc gtcgtctccg cagcaccatc ggcggcaccg gcctctccat
      541 ctccgcgacc agcagacacc ccaccgaatg cctcgccttc gcgcaatgga tcgtatcacc
      601 cgaatgccag tccaccttct ataccgaaca cggcggacaa cccggccacc ggcaggcctg
      661 gctcaacgaa aaaaccaacc gcctcaccca taactatttc tataccgtac tccccgccat
      721 ggaacggggc tacatgcgcc cccgctacca cggctacctc tatttccagg accacggcgg
      781 cgacccgatc cgcgaatacc ttctcgggaa caaagattgc cgcagcacgc tgcaatgcct
      841 cgaccagctg taccacgaaa gcctgaaaaa aaacccaacc acacaaacag tatgaaccag
      901 cttcctctca atgggctcac ggtactggaa ttctgccaat acctctccgg accctcggca
      961 ggtctccgcc tcgccgacct cggcgcccgg gttataaaga ttgagcgacc gggtacaggt
     1021 gacgcctgcc gccacctctc gatcaaaaat ctctgggtcg acggcgactc cctgctcttt
     1081 cataccatca accgcaacaa ggaaagcttc accgccgatc tcaaatcacc cgaaggcctg
     1141 aaaaacataa aaaaactcat cggcaccgcc gacgtcctca cccataactt ccgccccggc
     1201 gtcatggaag ccgccggcct cggcttcgaa gacataaaag ccctgaaccc ccgcctcatc
     1261 tacggcgcca tcagcggcta tggcacggaa ggcccctgga aggaccgccc gggccaggac
     1321 ctgctcctcc aatccatgag cggcctggcc tataccaccg gcaatgaaaa agacaacccc
     1381 atgcccttcg gcctctccat cgccgacagc ctttgcgggg cacaactcgt ccaggggatc
     1441 ctggccgccc tcatccgccg cggcaagacg ggtcagggcg cactggtcga gatcagcatg
     1501 atggagtccc tgctcgattt tcaattcgaa ttccttacca cctaccatag cagcgggcaa
     1561 ctgccgcgac gcagcaaaca aagcaacggc aatcccctgc tcagcgcacc ctacggcatc
     1621 tacgccacgg ccgacggcca tatcgccatc gccatggtca acatcccgca gctggcccag
     1681 gcagtcgatt cggccgctct cgccgcctac agccaggacc aggcctttag ccatcgcgac
     1741 gagataaagg gcctgctggc gcaacatttg acggcatacc ctacaaaata ctggctcgaa
     1801 cggctgcacg cggaggacat gtgggccatg gaagtgctcg actggcgcac cctgacggcc
     1861 caggaaggct acaagatcct ccagatggag caggcggtca ccacgacaag cggaaaacgg
     1921 gtcatcacca cccgctgccc cattcgcatc gacggccaac ggctcttctc ggagaaaccc
     1981 gccccggcgc tgggacagga taatgaaaag atcgtaaccg aactatcaaa gtaactaaat
     2041 ggatctccta aacgatatca tcgtcgtcga cttcagccag ttcctctccg gaccctccgc
     2101 cggcctccgc ctcgcagaca tgggcgcccg cgtgatcaag atcgaacggc ccggcatcgg
     2161 cgatatctgc cggcagctct acgtctcgga cacccgcatc gaaggcgaat cgaccatctt
     2221 ccatgcgatc aaccgcaaca aacaaagcta tgccgccgac ctcaaagaca agcacgatct
     2281 cgacaagata aaaaagctca ttgaaaaggc agacatcgtc ttacacaact tccgtcccgg
     2341 tgtcatggaa cgcatcggcc tcgactacgc aacggtaaaa gcaataaagc ccgatatcat
     2401 ctatggcgag gtcagtggct atggcgcgga aggcccctgg aaagacctcc cggggcagga
     2461 cctcctggtc caggcgctct cggggctcac ctggctgagc aacaaccgcg acgaacgacc
     2521 gacgcccatg ggagtctccg tcgtcgatat cctcgccgga acctacctga cccagggcat
     2581 cctcgcggcc ctgtatcgcc gggggatcag cgggcaaagc gccctcgtcc aggtaagcat
     2641 gatggaagcc atcctcgact tccagttcga agtgctcacc tgccattaca atgacggccg
     2701 gcaactgccc gtgagaagcc gcgtcagcaa cggcaatgcc tatatcccgg ccccctatgg
     2761 catctacaag acccgtgaag gctacctgtc gctggccatg ggcgatatcc tgaaactggc
     2821 cgcattgctc gaatgccagc ccctggggca gttctccgac agcgcagact ggttcccccg
     2881 tcgcgacgaa ataaaggcca tcctggccca acacctcctg ctccgtaccg ccgacgaatg
     2941 gctcgccatc ctgcagcccg ccgacatctg gtgcgcaaag gtgatggatt accacgatct
     3001 gctccagcaa ctgggctata aagttctgaa tatggaacta aaggtaaaga cgagcaacgg
     3061 cctttccatc cgtaccaccc gctgcccgat ccgtgtcgac ggcaaaatag ccgtatccga
     3121 gaaaggcgca cccctgctgg gcgaacacaa tgccctaatc gatgcgcaat tcgggctggt
     3181 aaagtaattt gctgtcctaa acatgcgcca ttatgaagga aaaattggtc gatacgcata
     3241 tcagggaaga attgatcgat acgcatagca aggaagaatt ggtcgataca catatccatg
     3301 tatggaattt cgatcaggcg gactacccct ggttgaaagg agacaccagt atcctgaacc
     3361 gttcctatga tctgtccggg ctcgaaccgc aacgatcacg cgccggcgtc acctccggcg
     3421 tcctcgtcca ggccgctaat aatcccgggg acacggcctg gatggtccgg acagcc
//
LOCUS       DATGFG010000118         4301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1005214, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000118 DATGFG010000000
VERSION     DATGFG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005214"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VKQ52_02220"
     CDS             <1..703
                     /locus_tag="VKQ52_02220"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HLZ86021.1"
                     /translation="IEFLISLGGDGTLLDTVALVRDKNIPVLGINFGRLGFLASIGRE
                     ELTTAVTALVNRTFVVDKRSLIHLDANRPLFGDVPYGLNEFAIHKTDTSPMIKIHTYL
                     NGEFLNTYWADGLIVATPTGSTGYSLSCGGPVVFPDSTSFVITPVAPHNLNVRPIVVP
                     DNNIISFEVEGRAENFICVLDSRKEIVDKQVQLAVRSEAFTLSLIRLNENNFLQTLRG
                     KLSWGLDTRNADGKF"
     gene            762..1607
                     /locus_tag="VKQ52_02225"
     CDS             762..1607
                     /locus_tag="VKQ52_02225"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HLZ86022.1"
                     /translation="MKKLLFGALVLLATLSASAQEQAIVHEGEFGFSAGAAQYFGDLN
                     PNPRFNTPNLALGGFFRKQFGNYVAVRIAAQYAFLAYSDKYNSYNEFMYRRNLSFNTN
                     IWELALMGDFNFFKFVPGSEYYRFTPYVTFGIGAFNYDPYTYYQGQKVYLRSLGTEGQ
                     GSSLYPARKPYGSMAVCFPLGVGVKYSLNRRMSLGFEVLYRFTTTDYIDDVSTTYAPD
                     ARAQFLPNGQPTLWYALQDRSYETGEPIGIKGRQRGYSNQKDSYITGQVTLAFNLTSY
                     KCPSY"
     gene            1777..2550
                     /locus_tag="VKQ52_02230"
     CDS             1777..2550
                     /locus_tag="VKQ52_02230"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HLZ86023.1"
                     /translation="MDVLKEKIDVKKLPRHIAIIMDGNGRWAQEKGQDRLFGHFHGVE
                     SVRDIVEGCAELGIGYLTLYAFSTENWDRPAYEVTGLMELLVDTIRKEVETLNKNNIR
                     LHVIGDMSMLPDYASLELKEALEFTAGNTGLNLVMALSYSSRWELTEAVRSLARDVQA
                     GRLDPDAITQDTIQQYLSTRDFPDPELMIRTSGEYRISNFLLYQLAYAELYFTSVRWP
                     DFRKKNLYEAILDFQGRERRFGKTSAQIQQDLMAPGSTF"
     gene            2679..>4301
                     /locus_tag="VKQ52_02235"
     CDS             2679..>4301
                     /locus_tag="VKQ52_02235"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HLZ86024.1"
                     /translation="MTDSTKKTSITDSLKTDTVKPAASDTTPHNISIDPDLVALASGK
                     NPPREYVIAGIKISGTKYLDESLLTSISGLTVGDKVTIPGGDNFSKAIQNLWKQNLFA
                     NISVYFTRLVGNSVYVEIDVTERPRLGNFYFKGPGVKKGDADDLTTKTGLIKGRVVTE
                     NMKRSAIQYIRKYYADKGYEDAQVRTTEARDPVVQNSEILTFYIDKGPKVRIYQIDFF
                     GNQAIREAKLKKQLKDTKEQSRMTIYPPDNQTPYGPHDSVTLRQYLNNWGFLSYHATK
                     DFMDPYFRFKMFSSAKFSASKFEDDKQKVVSYYNSLGYRDAVIVADTHYTDKRVRSRL
                     YVDVKVDEGHKYYFGNISWKGNTKYSDSILNLILGIHKGDVYNLDILNKKLGKQLSAE
                     GGDISGLYTDDGYLFFHVDPVETSVYNDTIDFEIRMVEGPQATIKNVTISGNDKTNEY
                     VIRRELFTIPGDKFSRTDLIRSQRQIANLGFFNQEKIQPGINPNQDDGTVDINWTVEE
                     KSGNQLELSAGWGGGIGLTGTVGITFANFSIKN"
BASE COUNT         1056 a         1115 c         1138 g          992 t
ORIGIN      
        1 catcgagttc ctgatcagcc ttgggggtga tggcaccctg ctcgatacgg tggcgcttgt
       61 ccgtgacaag aatatcccgg tgctggggat caacttcgga agattgggat tcctggccag
      121 tatcggccgg gaagagctga ccacggccgt tacggcgctg gtgaaccgaa cctttgtcgt
      181 ggataagcgt tcccttatcc atctggatgc caaccggcct ttgtttggcg atgtgccgta
      241 tggcctgaac gaatttgcca tccacaagac cgatacttcc ccgatgatca agatccatac
      301 ctacctcaac ggggagttcc tgaacaccta ttgggcggat gggttgatcg tcgccacgcc
      361 cacgggctcc accggttatt cgctgagctg cgggggacca gttgtgttcc cggattccac
      421 gagttttgtc atcacgccgg tggcgccgca caaccttaat gtccggccga tcgtagtacc
      481 ggataataat attatttctt ttgaggtgga aggccgtgcc gagaacttta tctgcgtcct
      541 cgattcccgg aaggagatcg tggacaagca ggttcaattg gctgtgcgga gtgaagcttt
      601 taccctaagc ctgatccggc tgaatgagaa caatttcctc caaaccctga gaggtaaact
      661 ttcctggggg ctggatacga gaaatgcaga tggcaaattt taacaaatcg tgtaaacgaa
      721 agggccggcc ctcttcgttt tgtataattc cactttttat catgaaaaag ctattattcg
      781 gcgccctggt acttctggca accttatctg cttcggcaca ggaacaggcc atcgttcacg
      841 agggcgaatt cggcttttcg gcgggggcgg cgcaatattt tggcgatctg aacccgaatc
      901 cgcggttcaa tacgccgaac ctggccctgg gcggcttttt caggaagcag ttcgggaatt
      961 atgtggcggt ccggatcgcc gcgcagtacg catttctggc gtattccgat aaatacaact
     1021 cctataacga gttcatgtac cgccggaatc tgagctttaa tacgaatatc tgggagctgg
     1081 cgctgatggg ggatttcaat ttcttcaagt tcgttccggg cagtgaatat taccggttca
     1141 ccccctatgt tacgtttggg atcggcgctt tcaattatga tccctatacg tattaccagg
     1201 ggcaaaaggt ttacttgcgc tcactcggta ccgagggaca gggctcgtcg ctttaccccg
     1261 ctcggaagcc ctatgggagc atggccgtat gcttcccgct tggcgtaggg gtcaagtaca
     1321 gccttaaccg ccgaatgagc ctggggtttg aagttttgta ccggtttacg accaccgact
     1381 atatcgacga tgtcagcacg acttatgcgc ctgatgcccg ggcccaattc ctgcccaatg
     1441 gacagccgac cctttggtat gccctgcagg accggtctta tgagaccggg gagccgattg
     1501 ggatcaaggg gcgtcagcgc ggatattcaa accaaaagga cagctatatc accggtcagg
     1561 tgaccctcgc ctttaacctg acatcctaca aatgcccaag ttattaacag cagggccgaa
     1621 cggagttcgg ccggccgaag gccagacgta ggccaaaaat cggccgggcg acttttaaca
     1681 actgtccggc cgattttcca gtattccccg gttcaaaagc cctatctttg tgccccaaaa
     1741 accggggaag agcccggctt taatcaaaaa gcctctatgg atgtgctgaa ggaaaagatc
     1801 gatgtaaaga agctgccccg ccatattgct atcattatgg atgggaatgg tcgctgggcc
     1861 caggaaaagg gccaggaccg gctattcggt cattttcatg gagttgagag cgttcgggat
     1921 atcgtggaag gctgtgccga actgggtatc ggctacctga cgctctatgc gttctccacg
     1981 gagaactggg accggccggc ctatgaggtc accgggctga tggagctgct ggtggacact
     2041 atccggaagg aagtagagac cctgaacaag aacaatatcc ggctgcacgt catcggggat
     2101 atgagcatgc tcccggatta tgcgagcctg gagttaaagg aagcgctcga gtttacggcc
     2161 ggcaacaccg ggctcaacct ggtgatggcc cttagctaca gcagccgctg ggaactgacc
     2221 gaagcggtcc gcagcctggc ccgtgacgta caggccggcc ggctggatcc cgatgccatt
     2281 acacaggaca ccatacaaca atacttgtct acgcgcgatt ttcccgatcc ggaattaatg
     2341 atccgtacca gcggcgagta tcgtattagt aattttctgc tatatcaatt agcttatgcc
     2401 gaattatact tcaccagtgt tcgttggcct gacttccgca agaaaaacct ctacgaagcg
     2461 atcctcgatt ttcagggaag agaaaggcgg tttggcaaga cgagtgctca aattcaacag
     2521 gatttgatgg ctcccggctc cactttttaa caaagacttt gtataaatcc ttttaaattg
     2581 ccgctctaaa aaccgactgg atgcaacgaa gatcgctgat ttgcgttttt atattgacca
     2641 cttactgctt actaaactcg ctcgctgctc tagcccaggt gaccgattct acaaagaaaa
     2701 catccataac cgattccctc aagaccgaca ccgtcaaacc tgctgcatcc gatactactc
     2761 ctcacaacat ctccattgat cctgatctgg ttgcacttgc aagtggcaag aacccacccc
     2821 gggaatacgt gatcgccggg atcaagatct ccggtacaaa atatctcgac gagtcgcttc
     2881 ttacctccat ttccgggctt actgttggtg ataaggtaac tattcccgga ggagacaatt
     2941 tcagcaaggc gattcagaat ctatggaagc aaaacctgtt cgccaatatc tccgtttact
     3001 ttacccgact ggttggcaat agcgtgtatg tggagatcga cgttacggaa cgtccccgcc
     3061 tcggcaattt ttatttcaag ggccccggtg taaagaaggg cgatgcggac gatcttacga
     3121 ccaagaccgg tcttatcaag ggccgggtgg tcacggagaa catgaagcgt tcggccatcc
     3181 aatacatccg gaaatactat gcggataagg gttatgagga tgcccaggtt cgtacaacgg
     3241 aagcccgcga ccccgtggta cagaactcgg agatccttac tttttacatc gataagggtc
     3301 caaaagttcg catttaccag atcgattttt ttggcaacca ggctatcagg gaagcgaaac
     3361 tgaagaaaca gctgaaggat accaaggagc agagccggat gacgatctat ccgcccgaca
     3421 accaaacacc atacggaccg catgactcgg taacgctgcg gcaatacctg aacaactggg
     3481 gtttcctgtc ctaccatgct acaaaggatt tcatggatcc ttatttccgg ttcaagatgt
     3541 tcagctcggc caagttcagc gccagtaagt tcgaggatga taagcagaaa gtggtatcct
     3601 actataactc tctcggctat cgcgacgccg ttatcgtggc cgatacgcat tataccgaca
     3661 agcgggtgag aagccggctg tatgtggatg tgaaggtcga cgaagggcac aaatattatt
     3721 tcgggaacat tagctggaag gggaacacca aatattccga ctccatcctc aacctgatcc
     3781 tgggtatcca caaaggggat gtctacaatc tcgacatcct gaacaagaag ttgggaaaac
     3841 agctctccgc ggaaggcggc gacatcagcg ggctgtatac ggatgacgga tacctgttct
     3901 tccatgtgga ccctgtagag acctctgtat ataacgatac gatcgacttc gagatccgca
     3961 tggtggaggg tccgcaggcg acgatcaaga acgtcaccat ttcgggcaac gacaagacca
     4021 acgaatatgt gatccggcgg gaactcttta ctattcccgg ggataagttc agccggaccg
     4081 acctgatccg aagccagcgg cagatcgcca acctgggttt cttcaaccag gaaaagatcc
     4141 agccggggat caacccgaac caggatgacg gaaccgtgga catcaactgg accgtcgaag
     4201 agaaatcggg taaccagctg gagctgtctg ccggctgggg cggcggtatc ggccttacgg
     4261 gtacggtggg gatcaccttt gccaatttct cgataaagaa c
//
LOCUS       DATGFG010000119         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1114925, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000119 DATGFG010000000
VERSION     DATGFG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114925"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VKQ52_02240"
     CDS             <1..1205
                     /locus_tag="VKQ52_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86025.1"
                     /translation="AFTSPSLANPDLKWETTLSRNIGLDATLFNNRFGFTVDLYSNKT
                     HNLLVNTPIPTNTGYTSQLQNVGSTTNKGVEVQFTATVLQRRAFQWSANFNISFNQNK
                     VTSLGPQLSYLQNSGWAGSGNPADYIIKVGQPVGAMWGLKSDGFYKTSDFDYNPSTRV
                     YTPKKGMVNDASITASTPMPGSLKYKSIDGDSLVTNNDRTIIGNAQPKFFGGLTQTFD
                     YHGFDASIFINFQYGNKVYNYNKLEFSSGYTPGANLMGFMKNRWHTVDANGNVVEGLN
                     STGQVVGASPDSLNRLNAGAKYWIPIVGSSSTTFQSNSWAVEDASFIRINNITLGYTL
                     PRSIVSKLKIARLRVYATVNNVAVITGYSGYDPEVSTRLSTPVTPGVDYSAYPRSRSY
                     VAGVNVTF"
     gene            1228..>2167
                     /locus_tag="VKQ52_02245"
     CDS             1228..>2167
                     /locus_tag="VKQ52_02245"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86026.1"
                     /translation="MQHKTPILRITALLLAVVFLLVGSCKKYLEPAAISSFSPDYVFS
                     NVQNAQKAVLGAYQDLEGDNGYGIRISMYFSYDNDEMMGIHQIGDNDRGDIAHYNASA
                     GNAQLFSPWNQLYQGIERANICIYNIPKMTLYTTGTPTQIGELKRLYGEALTLRAQYY
                     FELIRNWGDVPAQWVPSSQLTNFFLAKTDRDTIYNHLLDDLLQAESLVPWRTDVPSLG
                     DVLNDERITLGAVKGLRARIALYRGGYSLRRAGSIYGQTMARPADYKTYYQIAHDECQ
                     QIMARPDEHTLNPSFNTVFKGSIDAHTTDPVGEIIFR"
BASE COUNT          528 a          752 c          485 g          402 t
ORIGIN      
        1 cggccttcac atcgccttcc ctggcgaacc ccgacctgaa atgggaaacc accctctccc
       61 gcaacatcgg cctggacgct accctgttca acaaccggtt cggcttcacc gtagacctgt
      121 atagcaacaa aacacacaac ctcctggtca atacgcccat ccccaccaac accggttata
      181 ccagccagtt gcagaacgtc ggctccacca ccaacaaagg ggtggaagta cagttcaccg
      241 ccactgtcct gcaacgccgg gccttccagt ggagcgccaa cttcaacatc tccttcaacc
      301 agaacaaggt caccagcctc ggtccgcagc tttcctacct ccagaactcc ggctgggccg
      361 gcagcggcaa ccccgccgac tacatcataa aagtgggcca gcccgtcggc gccatgtggg
      421 gactgaaatc ggatggcttc tataagacca gcgacttcga ctataacccg agcacccgcg
      481 tctatacgcc aaagaaaggg atggtcaacg acgccagcat caccgcttcg acccccatgc
      541 ccggttccct caaatacaaa agtatcgatg gcgactccct cgtcacgaac aacgaccgta
      601 ccatcatcgg caacgcgcaa ccgaaattct tcggcggcct gacccaaacc ttcgattacc
      661 acgggttcga cgccagcatc tttatcaatt tccaatacgg caacaaggtc tataactaca
      721 ataagctgga attctccagc ggttataccc ctggtgccaa cctcatgggc ttcatgaaga
      781 accgctggca taccgtggac gccaacggta atgtcgtcga aggcctcaat tccaccggtc
      841 aggtggtcgg agcctccccg gatagcctga acaggctgaa cgcgggcgca aaatactgga
      901 tacccattgt tggctccagc agcaccacct tccagtccaa ttcctgggcc gtggaagacg
      961 cctcctttat ccggatcaac aatatcacac tcggctacac cctgccgcga tccatcgtca
     1021 gtaagctgaa gatcgcccgc ctgcgcgtct acgcaacggt gaacaatgtg gccgtgataa
     1081 cgggttacag cggctacgat ccggaagtaa gcacccgcct cagcacaccc gtcacgcccg
     1141 gcgtcgacta ttccgcttat ccccgcagcc gcagctatgt cgccggcgta aacgtaacct
     1201 tctaaacaga caataaacaa actctatatg cagcataaaa cacccattct cagaataact
     1261 gcgctcctgc tggcggttgt attcctcctg gtgggttcat gtaaaaaata cctcgagccc
     1321 gccgccatct cctccttcag tcccgactac gtcttcagca atgtgcagaa cgcccaaaaa
     1381 gccgtgctcg gcgcctacca ggacctcgag ggcgacaatg gctacggcat ccgcatcagc
     1441 atgtactttt cctatgacaa tgacgagatg atggggatac accagatcgg cgacaacgac
     1501 cgtggcgata tcgcccacta caatgccagt gccggcaacg cccagctctt ctctccctgg
     1561 aaccagctct accagggaat cgaacgcgcc aatatctgca tctacaacat cccgaagatg
     1621 acgctctaca ccaccgggac tccgacgcag atcggcgagc tcaaacggct atatggcgag
     1681 gcccttaccc ttcgcgcaca gtactacttc gagctgatcc gcaactgggg cgatgtaccc
     1741 gcccaatggg tgccctcttc ccaactgacc aatttcttcc tggccaagac cgatcgcgac
     1801 acgatctata atcatctgct cgacgatctc ctccaggccg aaagcctcgt cccctggcgc
     1861 accgatgtac cttcccttgg cgacgtcctc aatgacgaac ggatcacgct cggtgcagta
     1921 aaaggcctcc gcgcccgcat cgccctctat cgcggcggct actccttacg ccgcgccggc
     1981 agcatctacg gccagaccat ggcccgcccc gcggactata aaacgtatta ccagatcgcc
     2041 cacgacgaat gccaacagat catggcccgc cccgatgaac atacgctcaa tcccagtttc
     2101 aacaccgtat tcaaaggctc catcgacgcc cataccaccg accccgtggg agagatcata
     2161 ttccgcg
//
LOCUS       DATGFG010000120         4838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_92540, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000120 DATGFG010000000
VERSION     DATGFG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92540"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VKQ52_02250"
     CDS             <1..1379
                     /locus_tag="VKQ52_02250"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HLZ86027.1"
                     /translation="QAAESESLAFYLQALMHLGKEEESSRKAFVQRCWASMNKEERFV
                     FNKLITGGFRIGISQQTVVNALAKTAGAEAAVVAHRISGDWDPATTPFAALLSGEGAG
                     SEVSRPYPFYLAYALEGELVGLGEPGEWQAEWKWDGIRGQLIRRGDVLVVWSRGEELM
                     TEKFPEYQDFISRLPVGTVLDGEIIGLGSRPEVGAGAAEQGSGGMAAGLPFVVLPFAA
                     LQTRIGRKNITKKHLHEVPVGFIAYDLLEFGGEDWRSRPLAERRAQLENVVAAAGHPL
                     LPLSPVIVVDDWGQLATAREGARERGSEGIMLKRRSSPYQVGRKRGDWWKWKIDPLVI
                     DAVLVYAQKGHGRRSNLYTDYTFAVRDGDQLITFTKAYSGLTDKELAEVDAFVKKNSL
                     EKFGPVRTVKPELVFEIAFEGIAASSRHKSGVALRFPRINRWRRDKTPGDIDTLEALK
                     KMLEFYGK"
     gene            1393..2760
                     /locus_tag="VKQ52_02255"
     CDS             1393..2760
                     /locus_tag="VKQ52_02255"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86028.1"
                     /translation="MKKMMVLIALGAICVAFTTVLRMQQEDDPVPVPASPQRSGDAAK
                     GYAYLTTGDYIKSGIPYNYFFLGFPKNPANYLHRDSLNAVVPHEYTAVKAANGEIVVA
                     PNCLECHAQVFDGKLYVGLGNSMIDFTDRRKLNPRAAGMVENMLAKTDAKKYDAALPF
                     LRAMKVISGQLYTEVRGVNSADRLADLLVAHRDPQTLRWSNEPVIDVSGRVVPTDTPP
                     WWLLKKKHAMFYNGFGRGDFGRFLMASNLLTVSDSSEAREVDGHFNDVLAYIYSIQPP
                     KYPRPVDAALARKGGVLFVQYCSKCHGGEYPNLLIPESTIRTDSLLYKSNYQSPQFIA
                     WFNKSWFAQGDHPARLEPFDGYIAPPLDGIWITAPYLHNGSVPTLDGVLNSRLRPTYW
                     SRDFDHPQYDYEHVGWKYTREDSARGTTVYNTTLPGYGNYGHTFGDRLSAQERKAVIE
                     YLKTL"
     gene            complement(2770..3390)
                     /locus_tag="VKQ52_02260"
     CDS             complement(2770..3390)
                     /locus_tag="VKQ52_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86029.1"
                     /translation="MRNTLFLIAVILSFSACTTYQYVTFDSSGLQKDKQQLLTLENDT
                     MRVAYSFSGPGGNVTLTVFNKTSQPIYLDWTRSSLIRNDQSFTLRRRDTEIIPPQTSI
                     SDVMLNLNDEGGGIPRLNIPDSAHSRKYHYPSGGWIKYKAVDYTEAGSPIRLKAWLTF
                     LFGPGGSTDLTMTHSFYIGEVMHSGVYAGGFGPYHDPGDMLYVWYQ"
     gene            3441..>4838
                     /locus_tag="VKQ52_02265"
     CDS             3441..>4838
                     /locus_tag="VKQ52_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLZ86030.1"
                     /translation="MLLKETIGYRIIHEWMNTAGRTPFAFQEETWEHILAGRSGLVNA
                     PTGCGKTFSVFLGALIHFINTHPDDYLTRSGSGLQLLWVSPLRALAKDIGRAMEEVIR
                     GLGMRWQVGIRNGDTEMSERQKQKRRMPEVLIITPESLHLLLAQKGYPEVFQHVRIIA
                     VDEWHELLGSKRGVQVELALARIVWLKGGAVSVIGISATIGNLEQAKEVLLSSLRGVA
                     GNVAAGSEAAGDAAAAAGEANVRAQDFIVRAMIDKPIDVESILPDEIENYPWAGHLGL
                     KLIHKVIPILEKSRTTLIFINTRGMSETWYQSLLNVAPDLAGALALHHGSIDMELRNW
                     VEDALHEGKLKAVVCTASLDLGVDFRPVETVIQVGSPKGVARFLQRAGRSGHQPGQVS
                     KIYFLPTHSLELMEAAALKSAMAEGLIESREPMLLCFDVLIQYLSTLAISDGFYPGVL
                     FPELRSTFCYSEMTEE"
BASE COUNT          989 a         1161 c         1691 g          997 t
ORIGIN      
        1 cccaggcggc ggagagcgag tccctggcct tttatcttca ggccttgatg cacctgggca
       61 aggaggaaga gtcttccagg aaagcatttg tgcagcggtg ctgggcatcc atgaacaagg
      121 aagaaagatt tgtcttcaat aaactgatca cgggtggatt ccggatcggc atctcgcagc
      181 agaccgtggt caatgccctg gcgaagacgg ccggggccga ggcggcggtt gtcgctcacc
      241 ggatcagcgg agactgggac cctgcgacaa cgccctttgc ggccttgctg agcggcgagg
      301 gggcgggcag cgaagtgtcg aggccctacc ctttctacct ggcctatgcg ctggaagggg
      361 agttggtggg gcttggcgag cccggcgagt ggcaggccga gtggaaatgg gacggcatcc
      421 ggggacagct gatccggagg ggcgatgtgc tggtggtctg gagccgcggc gaggagctga
      481 tgacggagaa gttcccggag taccaggatt ttatatcccg gctgccggtg ggtacggtgc
      541 tcgatggcga gatcatcggg ctggggagcc ggccggaggt tggtgccggg gccgcggagc
      601 agggatccgg cgggatggcc gcgggcctgc cgtttgttgt tctgcctttt gccgcattgc
      661 agacgcgcat cggccgcaag aatatcacca aaaagcacct gcacgaggtt ccggttggat
      721 ttatcgccta cgatctgctg gagtttggag gcgaagactg gcggagccgg ccgctggcgg
      781 agcggagggc acagctggag aatgtggtgg ctgcggcggg gcatccgctg ttaccgttgt
      841 cgcccgtcat cgtcgttgac gactgggggc agctggcgac ggcccgggaa ggcgcacgcg
      901 aacggggcag cgaggggatc atgctcaaac gcagatcatc gccataccag gtggggcgga
      961 aacgggggga ctggtggaaa tggaagatcg atccgctggt gatcgatgcc gtgctggtct
     1021 atgcgcagaa gggacacggg cggcggagca acctgtatac ggattatact tttgcggtga
     1081 gggacgggga tcagctgata acgtttacca aggcctattc cgggctcacc gacaaggagt
     1141 tggccgaggt ggatgccttt gtaaagaaaa attcgctgga gaaattcgga ccggtgcgga
     1201 ctgtaaagcc cgaacttgtc tttgagatcg ccttcgaggg gatcgccgct tcttcgcggc
     1261 ataaatcggg tgtcgcgctg cgtttcccaa ggatcaaccg gtggcgcagg gataagacgc
     1321 cgggggatat cgatacgctg gaggccttga agaagatgct cgagttctat gggaagtagg
     1381 taaatttacg gtatgaagaa aatgatggtg ctgatcgcgc tcggggcaat ttgtgttgcg
     1441 tttaccaccg tgctgcgaat gcaacaggaa gatgacccgg tgcccgtgcc ggccagtccg
     1501 caacgaagcg gcgatgccgc gaaagggtat gcctacctga cgacgggcga ctatatcaag
     1561 agcggtatcc catacaatta tttctttctc gggttcccga agaatccggc caattatctg
     1621 caccgggaca gcctgaacgc cgtggttccg catgagtata ccgcggtgaa ggcggcaaac
     1681 ggcgagatcg tggtggcgcc caactgtctc gaatgtcatg cgcaggtgtt cgatggcaaa
     1741 ttgtatgtgg ggctggggaa ctcgatgatc gactttaccg atcggcggaa gctgaatccg
     1801 cgggcggcgg ggatggtgga gaatatgctg gcgaagacgg acgcgaagaa atacgatgcc
     1861 gccctgcctt ttctgcgggc gatgaaggtg atcagcgggc agttgtatac cgaggtgcgg
     1921 ggggtgaatt cggcggacag gctggcagat ctgctcgtgg cccatcgtga tccgcagacc
     1981 ctgcgctgga gtaacgagcc ggtgatcgat gtatcggggc gcgtggtgcc gacggatacg
     2041 ccgccctggt ggctgctgaa gaaaaagcac gcaatgttct ataacgggtt cgggcggggt
     2101 gatttcgggc gattcctgat ggcgtccaac ctgctgacgg tgagcgacag ttcggaggcg
     2161 agggaggtcg atgggcattt caacgatgtg ctggcctata tctattcgat ccagccgccg
     2221 aaatatcccc ggcccgtcga tgcggccctg gccagaaagg gcggggtgct ttttgtacaa
     2281 tactgctcca aatgtcatgg cggcgagtat cccaacctgc tgatcccgga atccaccata
     2341 cggaccgact cgcttttgta taaaagcaat tatcagagcc cgcaattcat cgcgtggttc
     2401 aataagagct ggtttgcaca aggggatcat ccggcgcggc tggagccctt tgacggctat
     2461 atcgcgccgc cgctggacgg tatctggatc acggcgccct acctgcataa cggcagcgta
     2521 cccacgctgg atggagtgtt gaacagcagg ctgcgcccca cttactggtc gcgggatttc
     2581 gatcatccgc aatatgatta tgagcatgta ggctggaagt atacacgcga ggacagtgcg
     2641 aggggaacga ccgtatacaa caccacgttg ccgggttacg ggaattatgg ccatactttt
     2701 ggcgaccggc ttagcgcgca ggaacggaag gccgtgatcg agtacctgaa gaccttgtag
     2761 ccgtttgggc tattggtacc agacgtagag catatcaccg gggtcatgat aggggccaaa
     2821 cccgccggca tagacgccgc tatgcattac ttccccgatg tagaaggagt gggtcatggt
     2881 cagatcggtg cttccccccg ggccgaaaag gaaggtgagc caggccttta accggatcgg
     2941 cgatcctgcc tcggtgtaat ccaccgcctt gtattttatc catccgccgc tggggtagtg
     3001 atacttgcgg gaatgggcgg agtcggggat gttcagccgg ggtatcccgc cgccttcgtc
     3061 gttgaggttg agcatcacat ccgagatgct ggtctgtggc gggatgatct cggtatccct
     3121 gcggcggagc gtaaaggact ggtcgttgcg gataagcgaa ctccgggtcc agtcgagata
     3181 gatgggctgg cttgtcttat tgaaaacggt cagggtgaca ttgccgcccg gtccgctgaa
     3241 actataggcg acgcgcatgg tgtcgttctc cagcgtcagg agttgttgtt tgtccttttg
     3301 tagcccggag gagtcgaagg tgacatactg ataggtggta caggcggaga aggagagtat
     3361 cacggcgata aggaataggg tgtttcgcat gaggaatctt tttgttaaag taataaagag
     3421 aacgttgggt ttcagttaaa atgctattaa aagagacgat aggataccgg ataatccatg
     3481 agtggatgaa tacggcgggg cgaaccccgt ttgccttcca ggaggagacg tgggagcata
     3541 tattggcggg gcgatcgggg cttgtcaatg cgccgacggg ctgcgggaag actttttcgg
     3601 tgttcctggg ggcgctgatc cattttatca atacacatcc cgatgattat ctgacgagaa
     3661 gcggcagcgg gttgcaactg ttatgggtat cgccgttgcg cgcgctcgcc aaggatatcg
     3721 gccgggcgat ggaggaggtg atcaggggac ttgggatgcg ctggcaggtg gggatccgca
     3781 atggagatac ggagatgagt gaacggcaga agcaaaagcg gcggatgccg gaggtgttga
     3841 tcattacccc ggagagcctg catctgctgc tggcgcagaa ggggtatccg gaagtgtttc
     3901 agcatgtacg gatcatcgcc gtggatgaat ggcatgaatt gctcgggagc aagcgtgggg
     3961 tacaggtgga gctggcgctg gcgcggatcg tgtggctgaa gggcggtgcc gtatccgtca
     4021 tcgggatcag tgcgacgatc ggcaatctcg aacaggcgaa agaagtgttg ctttcctcct
     4081 tgcgcggagt ggcgggcaat gtagcggcgg gcagcgaggc ggcaggggat gccgcggcgg
     4141 cggccggcga agcgaatgtg cgcgcgcaag attttattgt cagggcgatg atcgacaagc
     4201 cgatcgatgt ggagtcgatc ctgcccgatg agatcgagaa ttatccctgg gccgggcacc
     4261 tggggttgaa gctgatccat aaggtgatcc ccatcctgga gaagagccgg accacgctga
     4321 tctttatcaa tacgcggggg atgagcgaga cctggtatca gtccctgttg aatgttgcgc
     4381 cggacctggc gggtgcgctc gccctgcatc atgggtcgat cgatatggag ttacggaatt
     4441 gggtcgagga cgcgttgcac gaggggaagc tgaaggcggt ggtctgtacg gccagtctcg
     4501 atcttggggt cgatttccgg ccggtggaga ccgtgatcca ggtgggtagt ccgaaggggg
     4561 tcgcgcgttt tctgcaacgg gcgggacgta gcgggcacca gccggggcag gtgagcaaga
     4621 tctatttctt gcccacgcat tcgctggagc tgatggaggc ggcggcgctg aaatcggcga
     4681 tggcggaagg attgatcgag agccgggagc cgatgctcct ctgtttcgac gtgctgatcc
     4741 aatacctgag cacgctggcc atctcggatg ggttctatcc cggggtgttg ttccccgaac
     4801 tgcgatccac gttttgctat agtgagatga cggaggag
//
LOCUS       DATGFG010000121         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1025349, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000121 DATGFG010000000
VERSION     DATGFG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025349"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..742
                     /locus_tag="VKQ52_02270"
     CDS             74..742
                     /locus_tag="VKQ52_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HLZ86031.1"
                     /translation="MKSKTTTPLPRHSLADAVVGRLQQQLSLGVYKAGDKLPSEPELM
                     IEFGVGRSTIREAVRILANIGLVTVRQGSGTLVEAQRGIAEPLQQRLRRADAAHLDEV
                     RQLLEIKIAEKAALLRTRKDITKMKGLLEERKTAAEAGDTEGAIRSDIRFHVAMAEAS
                     RNDILADLYRSVAEQMGRHFRQTYLTTESFIETQQLHEQLLQSIISQDPKKAWQYAAK
                     ITAH"
     gene            753..2018
                     /locus_tag="VKQ52_02275"
     CDS             753..2018
                     /locus_tag="VKQ52_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ86032.1"
                     /translation="MSDTMTQSPATAVADTTTQKDAVVQKTVFSILLAISFSHLLNDT
                     MQSLIPSTYPLLKKSLNLDFSQLGLVTFSFQLTASLLQPFVGLYTDKKPQPYSLAAGM
                     CFTLAGLILLSRAGTFPLVLVAVALVGVGSSIFHPEASRLAYLASGGRRGMAQSVFQL
                     GGNAGASLGPLLAARIVSPYGQSNIIWFGFFALVAIGVLLKTGGWYSRNLGQRRKKAA
                     PAISPETRPGLPRTKVAIALFILLVLIFSKYFYMSSMSSYYTFFLIDKFHVSVQTSQM
                     MLFLFLFAVAAGTLIGGPVGDRIGRKYVIWFSILGVAPFTLLLPYANLFFTGVLSVCI
                     GLILASAFSAILVYAQELVPGKVGLIAGLFFGLAFGMGGIGAAVLGRLADRTSIDHVF
                     HLCAWLPLLGLLTGFLPDTSKPPRNRDGF"
     gene            complement(2041..2238)
                     /locus_tag="VKQ52_02280"
     CDS             complement(2041..2238)
                     /locus_tag="VKQ52_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86033.1"
                     /translation="MKKFFFVAAIAAFAACNNSGADTKAADSTKMASDSTKMSADSSK
                     MAMDSSKMAADTTKKDTATKK"
     gene            2447..3010
                     /locus_tag="VKQ52_02285"
     CDS             2447..3010
                     /locus_tag="VKQ52_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ86034.1"
                     /translation="MRTEINEQKLLEGLAHNDRKAIEAIYKQHYNMVQSLILSNNGYP
                     DDARDIFQEAMIVLYEKARSGSFELNSQLKTYLYSVCRRLWLKRLNQQQRFSSEVETL
                     EETVPVEEELERHEQRNKDFQVMEQSLRGLGEPCKSLLEAYYLEKRSMVEIAGDFGYT
                     NADNAKNQKYKCLMRLKKIFSQSKKNS"
     gene            3031..>3752
                     /locus_tag="VKQ52_02290"
     CDS             3031..>3752
                     /locus_tag="VKQ52_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLZ86035.1"
                     /translation="MQDAALLDAVERYLRGEMLPEEKVFFEHIRKTNPEVDQMVVDHA
                     AFLNQLTQYGETRRLKTQLDDVYQQLSREGQIEHPRPIAKVVTLWKKYKRTVFVAASI
                     AGITALFISGLISLVTPKVSQSSIDRLSRQLNDQAQKLKTVDSKLNNAVAASTTTFTR
                     GGTGFLVDGKGYLLTNAHVVKNATVVEVQNTLGAYQARIIQLDRQADLALLKIEDTSY
                     HAFNGLPYGINRGGADLGEELFT"
BASE COUNT          905 a         1174 c          880 g          793 t
ORIGIN      
        1 cgcggccccg aaaattccat ttttccattt cccggggagt ttttaccttt gctccaagtc
       61 atcagatgat acgatgaaat cgaaaacaac tactcctctt ccgcgccatt ccctcgccga
      121 tgccgtcgtc ggcaggcttc agcaacagct gtccctcggc gtctataaag caggcgataa
      181 actcccctcc gaacccgaac tgatgatcga gttcggcgta ggccgcagca ccatccggga
      241 agcagtccgg atactggcca acatcggcct ggtaaccgta cgccagggat caggcaccct
      301 ggtggaagca caacgcggca tcgccgaacc cctgcagcaa cgcctccgcc gggccgacgc
      361 cgcccatctc gatgaagtgc ggcaattgct ggaaataaag atcgcggaaa aagcagccct
      421 gctccgtaca agaaaggata tcacaaaaat gaaaggtctg ctggaggaaa gaaagacagc
      481 cgccgaggcc ggcgacaccg aaggcgcgat ccgcagcgac atccggttcc atgtcgccat
      541 ggcggaagcc tcccgtaacg atatcctcgc cgatctctac cgcagcgtcg ccgaacagat
      601 gggccgccat ttccggcaga cctatctcac cacggagagc tttatcgaga cccagcagtt
      661 acacgaacag ttattacaaa gcattatcag ccaggacccg aaaaaagcct ggcagtacgc
      721 ggcaaagatc accgcacatt aacttttata ccatgtcgga cacaatgacc caatccccgg
      781 caacagccgt tgccgacacc accacacaaa aagacgccgt cgtacaaaaa acggtattct
      841 ccatcctgct cgcgatcagc ttctcgcacc tgctcaacga tacgatgcag tccctcatcc
      901 cctccaccta tccgctgctg aaaaaatcac tcaacctcga ctttagccaa ctgggcctgg
      961 tcaccttcag cttccagctc acggcctccc tcctgcaacc cttcgtgggc ctctacaccg
     1021 acaaaaaacc acaaccctat tccctcgcgg ccgggatgtg ctttaccctg gccggactga
     1081 tcctgctctc gagagcgggt accttccccc tcgtactggt agccgtcgcc ctggtaggcg
     1141 tcggatcctc catcttccat cccgaagcct cccggctcgc ctacctcgca tcaggcggcc
     1201 ggcgcggcat ggcccagtcg gttttccaac tgggcggtaa cgccggcgct tccctcggac
     1261 cgctgctggc cgcccgtatc gtttccccct atggccagtc caatatcatc tggttcggct
     1321 tcttcgccct ggtggccatc ggcgttctcc tgaaaacagg cggctggtat agccgcaatc
     1381 tcggccagcg ccgcaaaaaa gcagcacccg ccatatcccc cgaaacccgg cccggcctcc
     1441 cccggacaaa agtggccatc gccctcttta tcctgctcgt gctcatcttc tccaaatact
     1501 tctacatgtc cagcatgagc agctattata cctttttcct tatcgataaa ttccacgtgt
     1561 ccgtccagac ctcgcagatg atgctcttcc tcttcctgtt tgccgtggcg gcggggacgc
     1621 tcatcggcgg acccgtcggt gatcgcatcg ggcgtaagta tgtcatctgg ttctcgatcc
     1681 tcggggtcgc ccccttcacc ctcctcctcc cctatgccaa cctcttcttc accggcgtac
     1741 tctccgtctg tatcgggctc atcctcgcct cggccttctc cgccatcctc gtctatgccc
     1801 aggaactggt ccccggaaaa gtagggctca tcgccggcct cttcttcggc ctcgcctttg
     1861 gcatgggcgg catcggagct gccgtcctgg gacgcctggc cgaccggaca agcatcgacc
     1921 acgtcttcca cctctgtgcc tggctccccc tcctcggcct gctcaccggc tttctcccag
     1981 acacgtcaaa gccgccccgg aaccgggacg gcttctaacg attgcttgcc atataaaaac
     2041 ttacttcttg gtagcggtgt ctttcttcgt agtgtcagca gccatcttgg aagaatccat
     2101 agccatcttg gaagaatcag cgctcatctt ggtggaatca gaagccatct tagtggaatc
     2161 agcggcctta gtgtcagcac cggagttgtt acaagcggcg aaagcggcaa tagcggctac
     2221 aaaaaagaac tttttcattt gattttttga ttttgcgtta attgaatggt ttcgtggtta
     2281 atactacccg ggaaaaaaag taacccgcaa taatcaatgt tttttgcata ttttttttac
     2341 taatacgtat gggctggctt ttgaggtcca aaagtgtttt ttttgaattt tttcccattc
     2401 ctttgggtta cgaatggtta atttgggtat taaatatagc acaagcgtga ggacagagat
     2461 caacgagcaa aagttactcg aaggactggc acataacgac cgtaaggcca tcgaagctat
     2521 ctacaaacag cactataata tggtgcaatc cctgatcctc agcaacaacg gctaccccga
     2581 cgatgcccgc gacattttcc aggaagccat gatcgtactg tatgaaaagg caagatccgg
     2641 ctcctttgaa ttaaatagtc agctaaagac ctacctctac tccgtatgcc gccggctctg
     2701 gctcaaacgc ctgaaccagc agcaaagatt ctcctccgaa gtggagactt tggaagagac
     2761 agtcccggtg gaagaggaac tggagcgaca cgaacagcgg aacaaagact tccaggtcat
     2821 ggaacagtcc ctaagaggcc tcggcgagcc ctgtaaaagc ctgctggagg catactacct
     2881 ggaaaaaaga agcatggtcg agatagctgg cgattttggg tataccaatg ccgacaacgc
     2941 aaagaaccag aagtataaat gcctgatgcg gttgaaaaaa atattcagtc agagtaaaaa
     3001 gaattcgtag atcaaaaata agtgagtaag atgcaagatg ctgccctatt agacgccgtc
     3061 gaacgctacc tccgcgggga aatgcttccc gaagagaaag tgtttttcga acatatccgg
     3121 aagaccaacc cggaagtcga ccagatggtg gtcgatcatg ctgcctttct gaaccagctc
     3181 acccagtacg gcgagacccg ccgcctgaag acacagctgg acgatgtata ccagcaactg
     3241 tcccgcgaag gccagatcga acacccccgc cccatcgcca aagtggtgac gctctggaaa
     3301 aaatacaaac gtaccgtttt cgtcgccgcc tccatcgccg gcattaccgc cctctttatc
     3361 agcggtctca tctcactggt tacaccaaag gtcagccagt cgagtatcga ccggttaagc
     3421 cgccagctca acgaccaggc gcaaaaactg aaaaccgtag acagcaaact gaacaacgcc
     3481 gtggccgcct cgaccactac cttcacccgc gggggcaccg gctttctcgt ggacggtaaa
     3541 ggctacctgc tcaccaacgc acatgtagta aagaacgcca ccgtcgtcga agtgcaaaac
     3601 accctcggag cataccaggc ccgcatcatc cagcttgacc gccaggccga tctcgccctg
     3661 cttaagatag aagacacctc ctatcatgcc ttcaacggcc tgccctacgg tatcaacagg
     3721 ggaggcgccg atctcggtga agaactcttt ac
//
LOCUS       DATGFG010000122         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_203067, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000122 DATGFG010000000
VERSION     DATGFG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203067"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1291)
                     /gene="selD"
                     /locus_tag="VKQ52_02295"
     CDS             complement(164..1291)
                     /gene="selD"
                     /locus_tag="VKQ52_02295"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HLZ86036.1"
                     /translation="MRKYCSGRRNHWQQQQTSIDKHSMETTSMEIKLTQYSHGAGCGC
                     KISPAILDKILHSELPVAADPRLLVGNDKRDDAAVWDMGNGSALISTTDFFMPIVDDA
                     YDFGRIASANAISDVYAMGGRPVLAIAILGWPVDKLPPGIAQRVLEGARAICAEAGIS
                     LAGGHSIDCPEPVFGLAVNGIVPVHRIKRNSTAVEGCRLYLTKALGVGVLSTAQKRGL
                     LQPEDAAIALKSMVSLNKLGETLGQLEGVKAMTDVTGFGLLGHLTEMCEGSGLSATIE
                     FGKVPVIPSLSGYLAQGCIPGGTQRNWASYGSKIKLRSDEQKYTLADPQTSGGLLIAV
                     TPDAAPELEQVLRQHGLPAANCIPFGRLRAQDEESLIEVVE"
     gene            complement(1230..2279)
                     /gene="mnmH"
                     /locus_tag="VKQ52_02300"
     CDS             complement(1230..2279)
                     /gene="mnmH"
                     /locus_tag="VKQ52_02300"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HLZ86037.1"
                     /translation="MTRRITIAEWQALPDRPPVADVRTPAEFAHGHIPGAFNIPLFTN
                     EQRVEVGITYKQKGKEAAILLGFDLTGSKWSGFIHQALGMAPQKKIAVHCWRGGMRSA
                     AMAWALDLYGFEVFLIQGGYKSYRRWVLQQMEQPWRLSVVGGMTGSGKTRILAQLHRL
                     GEQVVDLEDLAQHRGSTYGTLNRLVQPTQEQFENNLAAQLSAMDPLLPIWIEDESQNI
                     GKCLLPKPFWTQLRNAILFDLQVPLECRVAALVEEYGGLDRDFLVECTERISRRVGPE
                     QTKHAVAAIREGRIPDFIRLVLVYYDKTYQKGLKSRDTARIIELTLAGGDPLPNAEVL
                     LRAAQPLATTTDLHR"
BASE COUNT          447 a          679 c          722 g          558 t
ORIGIN      
        1 caacaccgcg ggggcagaat ccagacaacc tgatcgcgat gttattatcc caaacatcgt
       61 gctaaaacgc gaaaaccttt ctgtataagc gatttaggcg gttcgccggc atgaagtcca
      121 tggaactatt tactccagcg gaaaaacatt ttgtttgttg catttattca accacttcga
      181 tcaggctctc ttcgtcttgc gccctgagcc ggccaaacgg gatgcagttg gccgcgggga
      241 ggccgtgctg acgtaacacc tgttccagtt cgggcgccgc atcgggtgta acggcgatca
      301 gcagtccccc gctcgtttgc ggatcggcaa gggtatattt ctgttcgtcc gaacggagtt
      361 ttattttgct cccatagctt gcccagttgc gctgggtgcc gccggggata cagccttgcg
      421 ccaggtagcc cgatagggag gggatcaccg gcaccttgcc gaactcaatc gtcgccgaga
      481 ggccgctgcc ctcgcacatt tcggtcagat ggccaagcag gccgaagccg gtgacatcgg
      541 tcatggcctt tacgccttcg agctgaccga gggtctcgcc cagtttattc aagctcacca
      601 tgcttttcag ggcgatggcg gcgtcttcgg gttgaagcag gcctcttttc tgcgccgtgg
      661 aaaggacgcc gacgcccagg gcctttgtca ggtataggcg gcagccttcc actgccgttg
      721 agttacgttt tatccgatgg acaggaacga tgccgttcac ggcgagaccg aagacgggtt
      781 cggggcagtc gatgctatgg ccaccggcca ggctgatccc cgcttcggca caaatggccc
      841 ttgccccttc cagcactcgt tgggctatcc cgggcggtag cttgtccacg ggccagccaa
      901 ggatggcgat cgccagtacc ggccggccac ccatcgcata tacgtcgctg atagcattgg
      961 ccgaagcgat acggccgaaa tcgtaggcgt cgtccacgat cggcataaag aaatccgtcg
     1021 tcgagataag ggcggagcca ttccccatat cccatacggc tgcgtcatcc cgtttatcat
     1081 tgccgacaag caggcgcgga tcggcggcaa cgggtagttc gctgtggagg atcttatcga
     1141 ggatggccgg gctgatctta catccgcaac cggccccatg tgagtattgt gtcagtttta
     1201 tttccatgct tgttgtttcc atgctgtgtt tatcgatgga ggtctgttgt tgttgccagt
     1261 ggttgcgccg cccggagcaa tacttccgca ttggggagcg ggtcgccgcc ggcaagggtt
     1321 agttctatga tccttgctgt atcccgcgat ttcaagcctt tctgataggt tttatcgtag
     1381 tataccaata cgagacggat aaaatccggg atgcggcctt cccggatggc ggctaccgcg
     1441 tgtttggtct gttccggtcc cacccggcgg ctgatgcgtt cggtgcattc gaccaggaaa
     1501 tcgcggtcca gtccgccgta ctcttccacg agggcagcta cgcggcattc cagcggcacc
     1561 tgcagatcga agagaatggc gttgcgcagt tgggtccaga agggctttgg caggagacat
     1621 ttcccgatgt tctggctttc gtcttcgatc cagatgggga ggagggggtc cattgccgag
     1681 agttgggccg ccaggttgtt ctcgaactgt tcctgtgtcg gttgcactag ccggttgagt
     1741 gtgccatagg tggagccacg gtgctgggcc aggtcctcca ggtcaaccac ctgctcgccg
     1801 agccggtgga gttgtgccag tatccgggtc ttgcccgagc ccgtcatgcc gcccaccacc
     1861 gagagccgcc agggttgttc catttgttgc agcacccacc gccggtaact tttatatccg
     1921 ccctggatca ggaagacctc gaagccatag aggtcgaggg cccaggccat cgcggcactt
     1981 ctcattcctc cgcgccagca gtgcacggcg atctttttct gtggtgccat cccgagcgcc
     2041 tggtgaataa agccggacca tttcgacccg gtgagatcga agcccagcag gatggcggct
     2101 tccttacctt tttgtttgta ggtgattccc acctccaccc gctgttcgtt cgtgaagagg
     2161 gggatgttga acgcacccgg gatatggcca tgggcgaatt ccgcgggggt gcggacgtcg
     2221 gccaccgggg gacggtccgg gagcgcttgc cattcggcta tggtgatcct gcgagtcata
     2281 ctgggtgccg gcggtggccg gggggtaaat ataagatgaa tgggggaatc cagtatattt
     2341 agccttattc ctaaaaaact tcttctatga atgagcaagc agtcgtcttt aagaccatcc
     2401 ccgtcg
//
LOCUS       DATGFG010000123         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_92236, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000123 DATGFG010000000
VERSION     DATGFG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92236"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VKQ52_02305"
     CDS             <1..452
                     /locus_tag="VKQ52_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86038.1"
                     /translation="FEKYLPEMYDVFAADKDKFNSPEEKKALEHIYPLCTNISIDFGI
                     MEKADNVYVIPSSFGWSDLGTWNSAYENLEKDYLGNAVAGDNVVVIDAVRCMVHAPND
                     KLLLLQGLDDFIVVDTKDVLLICKREKEQEIKEYVAEIKRNKGDQYL"
     gene            482..1216
                     /locus_tag="VKQ52_02310"
     CDS             482..1216
                     /locus_tag="VKQ52_02310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLZ86039.1"
                     /translation="MTPHPLHSVLFLDIETVSQYPAFENVPEEWKALWRHKAENLIRD
                     KPALTPEEIYGRAAIYAEFGKIICISVGLVSGAGTEKKISIKSFSGDDERTLLDQFSA
                     MLGKWSTPNQKYDSQRYLCAHNGKEFDFPYLCRRMIIHGLPIPSLLNIAGKKPWEIAH
                     IDTMELWKFGDYKSFVSLNLLAHVLGIPTPKDDIDGSQVGEAYWSRHELTRIVEYCQK
                     DVITVAQVYLRMHGETAISPANIEIR"
     gene            1273..>2153
                     /locus_tag="VKQ52_02315"
     CDS             1273..>2153
                     /locus_tag="VKQ52_02315"
                     /inference="COORDINATES: protein motif:HMM:NF006569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HLZ86040.1"
                     /translation="MSSLAAELGAVNLGQGFPDFPMSSELIALVNEAMNNGFNQYAHM
                     NGLPSLREVLAEKVGYLYGTKIDPGSQITITPGGTYAIYTALTAVLRPGDEVIVFEPA
                     YDSYIPNITVNGAIPVRIDLRFPDYKIDWEEVRRRISPRTRMIMLNSPHNPTGAVLDA
                     SDMEALHSIVRGTDIFILSDEVYEHLIFDDIPHQSILRYPALLERSFVCFSFGKVYHC
                     TGWKLGYCISAPDLMAEFRKVHQFNCFSCHTPSQVALSKYLTDKETFLSLSGFMQAKR
                     DYFRRLMESTRFSPLPS"
BASE COUNT          561 a          515 c          537 g          540 t
ORIGIN      
        1 cttttgaaaa gtacctgccg gagatgtatg acgtttttgc tgcagataag gacaaattca
       61 attccccgga agaaaagaag gccctcgaac atatttaccc tctctgcact aatatttcca
      121 ttgatttcgg gatcatggaa aaggccgaca atgtttatgt cattccctcc tcattcggat
      181 ggagcgatct gggcacctgg aacagcgcgt acgaaaacct tgaaaaagac tacctgggca
      241 atgccgttgc cggagataac gtcgtagtga tcgatgccgt cagatgcatg gtacatgctc
      301 ccaatgataa attgctcctt ttacaggggc tggatgattt tatcgtggta gataccaagg
      361 atgttctgtt gatctgcaaa agagagaaag agcaggagat caaagaatat gtggcggaaa
      421 tcaaacgaaa caagggagat cagtatcttt aaggaacaga accaaaagtt ttttcctttc
      481 tatgactcct catccgctcc atagtgtctt attcctggac atagaaacag tgtcgcaata
      541 cccggcgttt gaaaatgtac cggaagaatg gaaagcccta tggcggcata aggcggaaaa
      601 cctcatcagg gacaagcccg cgcttacccc ggaagaaata tatggtcgcg cggcgatata
      661 cgctgaattt ggcaagatca tttgcatcag cgtcggactc gtctcaggcg ccgggacgga
      721 gaagaagatc tcgatcaagt ctttttccgg ggatgatgag cgaacgctcc tcgatcagtt
      781 tagcgcaatg ctcggtaaat ggtcgacgcc gaatcagaaa tacgatagcc agaggtactt
      841 gtgtgcgcac aacgggaaag aattcgactt tccttatctg tgccgccgga tgatcataca
      901 tggactgccg attccttccc tgctgaatat tgccggtaaa aagccctggg aaatcgccca
      961 tatagatacc atggagcttt ggaaattcgg agattacaaa agttttgtct ccttgaacct
     1021 gctggcccat gttctgggta taccgacccc caaagacgat atagatggca gccaggtagg
     1081 tgaagcttac tggtccaggc atgagctgac acgcatcgtt gaatattgcc agaaagatgt
     1141 aatcactgtg gcacaggtct acctccggat gcatggagaa accgccatat cacctgcaaa
     1201 tatagagatt agatgagccg tccttcaatg caaagcaagc ttccacaggt tggaacgacg
     1261 atatttactg tcatgagctc tctcgctgcg gagttgggcg cagtgaacct gggacaaggt
     1321 tttccggact tccctatgag cagtgagctg atcgcgcttg tgaatgaggc catgaacaat
     1381 ggattcaatc aatatgccca tatgaacgga ttgccgtccc tccgggaagt gctagcggaa
     1441 aaggtaggct acttatatgg cacgaagatc gatcccggat cgcagatcac cattacaccc
     1501 ggcggcactt atgcaattta taccgctttg acggctgtgt tgcggcccgg agacgaagtc
     1561 atcgtttttg aacctgccta tgacagctat ataccgaaca tcacggtcaa cggtgctatc
     1621 ccggtaagga tcgaccttcg cttcccggac tataagatcg attgggaaga agtccgccgc
     1681 aggatctccc ctcgtacccg catgatcatg ttgaatagtc cgcacaatcc tactggagcg
     1741 gtgcttgatg cgtcggatat ggaggcatta cattcgatcg tccggggaac ggatattttc
     1801 atcctgtctg acgaagttta tgagcacctc atctttgatg acattcccca tcagtccata
     1861 ttgaggtatc cggcgttgct ggaaagaagt tttgtatgtt tttccttcgg caaggtctat
     1921 cactgtaccg gctggaaact tggttattgc atatctgccc cggacctgat ggccgagttc
     1981 aggaaagtgc accagttcaa ttgcttcagt tgccatacac cttcccaggt ggccctgtca
     2041 aaatacctga cggataagga gacctttctt tctctatcgg gttttatgca ggcgaagagg
     2101 gattatttcc ggcgcctgat ggagtccacc cgcttttctc ccctaccgtc tta
//
LOCUS       DATGFG010000124         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1701913, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000124 DATGFG010000000
VERSION     DATGFG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701913"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..838
                     /locus_tag="VKQ52_02320"
     CDS             125..838
                     /locus_tag="VKQ52_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86041.1"
                     /translation="MKTLIKPSVIVLAAVLACTTLYAQSADEIVTKHVAAIGGKEAVD
                     NVKTLYIESDVDVAGNNAPSITWIVNGKGYKNEVDFNGSKIQQCVTDKGGWMLNPMMG
                     QSSAQPMSDDQLKAMKGQISVGGPLYDYAGKGNKIESLGQDTADYKIKLTTPAAVAVT
                     FYINKKTYLIDKTVSNATIQGQEMETTVIFSDYRKLDGGYVLNFGQQVTLPMYTLNIT
                     HKKVEVNKPMDPTIFDMPK"
     gene            complement(953..1900)
                     /locus_tag="VKQ52_02325"
     CDS             complement(953..1900)
                     /locus_tag="VKQ52_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86042.1"
                     /translation="MTISGFTMVRNATKLYYPIKQAISSILPIVDEFVVALGDCDPDD
                     RTLQEIQSIGSDKIRIIHTVWDLDKFPKGMEHAHQTDIAKSACKGDWLFYVQSDEVVH
                     EDDLPKIRQRCEELLYDESIDGILFKYRHFWGDYDHYITSHAWYANEIRIVRNDKEIH
                     SWESAQSFRRIPEFDGVHYREKKGTYKLKVARVDAYIYHYGWVRPPQYMQKKRKAFAT
                     IHSGKTRAEEYFRKQEEEFDFGNLNQLSIYQDTHPGVMQERIRLFNWADQLERRRFRQ
                     PVVRHKHERLKYRMLTFVEQNFLGGRQIMCFKNYRLVSR"
     gene            complement(2549..>3075)
                     /gene="trmD"
                     /locus_tag="VKQ52_02330"
     CDS             complement(2549..>3075)
                     /gene="trmD"
                     /locus_tag="VKQ52_02330"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HLZ86043.1"
                     /translation="GGGAGMVMMAEPLANAIEALSQGGAFDEIIYLTPDGERLNQKTA
                     NGLSLMGRLLLICGHYKGIDQRIRDKYVTREISIGDYVLSGGELAAAILVDTIGRLIP
                     GVLNDETSALFDSFQDNLLAPPVYSRPAEWRGMRVPEILLSGDPRKIEDWRHEQSLQR
                     TRQRRPDLLEGEED"
BASE COUNT          768 a          746 c          800 g          761 t
ORIGIN      
        1 tctctttttg catagaaata aggtttaaat gaaaggttag gttaatttta tgaaaggtaa
       61 caccctgcag gcaaatattg gctaagtttg ttgtatggta aatatatttt ataaaaaaaa
      121 atacatgaaa acactcatta aaccatccgt cattgtacta gcggccgtcc ttgcctgcac
      181 tactctgtat gctcaatcgg ctgacgagat cgtcaccaag catgtagctg ccatcggtgg
      241 caaggaagct gtagataatg taaagacgtt atatatcgaa agtgatgtgg atgtggcagg
      301 caataacgca ccgagtatta cctggatcgt caacggcaag ggctataaga acgaggtaga
      361 cttcaacggc agtaagatcc agcaatgcgt caccgacaag ggtggctgga tgctcaatcc
      421 gatgatggga cagagctcgg cgcaacccat gtccgatgat cagttgaagg cgatgaaggg
      481 gcagatcagt gtgggcggac cgctgtatga ctacgccgga aaaggcaata agatcgaatc
      541 cctcgggcag gataccgcag attataagat caagctgacg acgcctgcgg cggtggcggt
      601 gaccttctat atcaacaaga agacctatct catcgacaag accgtcagca acgccaccat
      661 ccagggccag gaaatggaga cgacggttat tttttccgac tatcggaaac tggacggggg
      721 atatgtcctt aactttggtc agcaggtgac cctgccgatg tataccctta acataaccca
      781 taagaaagtt gaggtaaata aaccgatgga tcctactata tttgatatgc ccaagtaaat
      841 cccgcgagtc gacctatccc aataagaaga agagccgttg tataaattac agcggctctt
      901 cgtttatgta ggaaaccgtc cagtggttta ttggtggaac gccgagacgt ctctaccggc
      961 tgacgaggcg atagttctta aagcacatga tctgtcttcc tcccaaaaaa ttctgctcga
     1021 cgaaggtcag catccggtat ttcagccgtt cgtgtttgtg acggacgaca ggctggcgga
     1081 aacggcgccg ttcgagctgg tcggcccaat tgaaaagccg gatacgttcc tgcatgacac
     1141 cgggatgggt atcctggtag atcgacaact ggttcagatt gccaaagtcg aattcctctt
     1201 cctgcttccg gaaatattcc tcggcgcggg tcttgccgct atggatcgtg gcgaaggcct
     1261 tgcgtttctt ctgcatatac tggggcgggc ggacccagcc gtaatgatag atgtaggcat
     1321 ctaccctggc gaccttcagc ttgtaggtgc cttttttctc ccggtagtgg accccgtcga
     1381 actccggtat ccggcggaag gactgggccg actcccagga gtgaatttcc ttgtcattac
     1441 ggacgatcct gatctcattg gcgtaccagg cgtgggaagt gatatagtgg tcataatccc
     1501 cccagaaatg ccggtacttg aaaaggatgc cgtctatcga ctcgtcgtac aatagctctt
     1561 cgcagcgttg acgaatcttt ggcagatcat cctcgtgtac cacctcgtcg ctctggacat
     1621 aaaaaagcca gtcgcccttg caggcggact tggcgatatc ggtctggtgg gcatgctcca
     1681 tccccttggg aaacttatct aagtcccaga cagtgtggat gatacgtatt ttatccgagc
     1741 cgatgctctg gatctcctgg agggtacggt cgtcggggtc gcaatcccca agcgcaacga
     1801 caaactcgtc aacgatgggg aggatagagg aaatagcctg tttgatcggg taatacagct
     1861 tggttgcgtt acgcaccatg gtaaagccgc tgatagtcat agccgttttt tttgtgtttc
     1921 aagtttcaat taggagtaga agactactat tctacataga cgaacaattt ccgtgcaggg
     1981 ttggaccaaa aagattaaga gaagattaaa cgctgatagg cggagaattg tataaaggcc
     2041 agtggtttaa caaatggata actggtaatt aacgtgttaa taccgcaaaa ggcccggtaa
     2101 ataaggggtt tgggagacat taaaaatcga ttaaaaaatt tgtgaaaatt aggctggggc
     2161 atttagcagc ctgcagcgag gtttctgcaa gggtattttc aaggggttcc gggtacaatt
     2221 cctatgtgga taaactcgaa ttggtatagt tttcgccttt tagaggtagg ctgccaatca
     2281 gttcattagc cgttaaacct tttcttttat cctgttttat ccagaacgga ccacgggtcc
     2341 gttggccgaa ggcccatttt tagccatttt gacgccgtgg ctaattttgc taagagccac
     2401 tgggcgtcaa aatcaaaatc cgttccttat gagaaagacg atcgttatca acacgctcct
     2461 tatcctgggg tactatctta tcttgcagct gttgatctgg gtggcgatga cattacgata
     2521 agagtcgact cgaatacaga cccaaggatc aatcctcttc tccctccagc agatcgggcc
     2581 ttcgttgcct ggtacgctgg agggattgtt catgcctcca gtcttcgatc tttcgcgggt
     2641 cgccgctaag caggatctcc ggcacccgca tcccccgcca ctccgccggg cgtgagtata
     2701 cgggcggggc cagcaaattg tcctggaagg agtcgaacag ggccgaggtc tcgtcattga
     2761 gcaccccggg gatcagtctc ccgatcgtat cgacaaggat ggccgccgcc agttcgcccc
     2821 cactgagcac ataatccccg atcgagatct ccctggtcac atatttgtcc ctgatccgct
     2881 ggtcgatgcc cttgtaatgc ccgcagatga gtaagagtct acccatcagc gaaaggccgt
     2941 tggcggtctt ttggttgagc cgttcgccgt ccggggtcag ataaatgatc tcgtcgaatg
     3001 cgccaccctg tgagagcgct tcgatcgcat tggccagcgg ttcggccatc atcaccatac
     3061 ccgccccccc gccat
//
LOCUS       DATGFG010000125         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1576582, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000125 DATGFG010000000
VERSION     DATGFG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576582"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VKQ52_02335"
     CDS             <1..225
                     /locus_tag="VKQ52_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86044.1"
                     /translation="GGMGRGLPGQNNANPLPLPVPKVAHAVGILAGNSSGLALLQDGT
                     VMGWGGDIVSAREGGDYRRTWTPVVMTRRY"
     gene            338..871
                     /locus_tag="VKQ52_02340"
     CDS             338..871
                     /locus_tag="VKQ52_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86045.1"
                     /translation="MKKAPKLTLAFLLSLAIPAVVLSQPARILRDSGLRKLSVFIGKW
                     KAESTIDANHKDKISAVYTCRWSDNGRYMIADQLVNNNGKETNNLSIYNYNADKGDYT
                     LSLVGIPGVQPFSIGVQARGDTLFYNSEYTDNGKKMYNRTLNIFASPTSYIYKVQFSD
                     DGVTWRTDGEGSARKMP"
     gene            complement(876..>2030)
                     /locus_tag="VKQ52_02345"
     CDS             complement(876..>2030)
                     /locus_tag="VKQ52_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ86046.1"
                     /translation="LGVVYYNLKRTLEAEKAFLNAIQRNGSLFDSWLGLGNVYENLDR
                     YEEAENAYKQAYQLDDQSTLPLIKLARHFHYRMEKYDEAEKTYRRILELDKFLYEEWS
                     DLGDLYQYELQKYSEAEQAYEKAIEIKPGWPDPWSGLGDLYQNYFGRYQEAEHKYKKA
                     IELQPDWEQPWAGLGDLYQEHFNKYPEAEHAYKKAIDLDPSFDDCWHRLGMLYQNHMG
                     KFMEAEQAYEKALELDPDNDAAKHNLIFLWRDKLNKPIQAKDLFKSLERSADIDDTYF
                     LNEALFGYYEKNIGLAEGSLSNALAATNGSLSPDSIDDWYRSASVIIRLGFSESIQRV
                     FEDTGYDAIYKPFYIAIKALAEKDGDLFLNSIAAEVREPAMTIMKLIESC"
BASE COUNT          470 a          505 c          506 g          549 t
ORIGIN      
        1 gggggaatgg gccggggctt gccggggcag aacaacgcaa atcctttacc gttgccggtt
       61 cccaaggtgg cacatgcggt ggggatcctg gccgggaata gttcgggcct tgctcttttg
      121 caggacggca ccgttatggg ctggggggga gatattgtgt cggcgcgcga aggcggcgac
      181 tatcggcgga catggacacc cgtagtgatg acgagacggt attgaggcgg gattgaggcg
      241 gtattgaggc ggccttgcca ccctcctttg tttcctcgcg gaacaatgcg tcaattcatc
      301 ggttgctttt ttgtatcttg tatgacaagc acaaactatg aaaaaagccc ccaaactaac
      361 ccttgccttt cttttatccc tggcgattcc cgccgtcgtc ctcagtcagc ctgccaggat
      421 actgagggac tccggtctgc gaaaactcag cgttttcatc ggcaaatgga aggccgaaag
      481 caccatcgac gccaaccaca aggacaagat ctccgccgtg tatacctgcc ggtggagcga
      541 taacggccgg tacatgatcg cggaccaact cgtcaacaac aatggtaagg agacgaataa
      601 tctctccatt tataattaca atgccgataa gggtgattat acgctttcgc tggtgggcat
      661 cccgggcgtg cagccgttta gcatcggcgt ccaggcaagg ggcgataccc tcttctacaa
      721 cagtgagtat accgacaatg ggaagaagat gtacaatcgg acgttgaata tctttgcctc
      781 gcccacaagc tatatctaca aggtccaatt ctcggacgat ggggtcacct ggcgcacgga
      841 tggggaagga tcggcgagga agatgccgtg agaattcagc aactctcaat cagcttcatg
      901 atagtcatag ccggctcccg gacttcggct gcaatgctgt tcaggaaaag atcgccgtct
      961 ttctccgcca gcgcttttat ggcgatgtag aaaggtttat agattgcatc ataaccggta
     1021 tcttcaaata cgcgctggat gctttccgaa aaaccgagcc ggataatgac cgaggcagat
     1081 cggtaccagt cgtcgatgga atccggggat aagctgccat tggttgcggc cagggcgttg
     1141 cttaaggaac cctctgccag tccaatgttt ttttcatagt agccaaataa tgcctcgttt
     1201 aaaaagtaag tgtcatctat atctgctgag cgttcaaggg atttgaatag atctttggcc
     1261 tggatgggtt tatttaattt atcccgccag aggaaaatga ggttatgctt agccgcatca
     1321 ttgtctggat ccaattccaa cgccttttca tatgcttgct ccgcttccat gaatttgccc
     1381 atatgatttt gatatagcat acctaagcga tgccagcaat cgtcgaatga cgggtctaga
     1441 tcgatggcct ttttgtaggc atgctcggcc tccggatatt tgttgaagtg ttcctggtat
     1501 agatcgccaa gtccggccca gggttgttcc caatccggtt ggagctctat cgcctttttg
     1561 tatttatgtt ctgcttcctg atacctgcca aaatagtttt gataaagatc gcctagtcca
     1621 ctccagggat caggccaacc cggttttatt tctatggcct tttcgtaggc ctgttctgcc
     1681 tcggaatatt tttgaagctc gtattgatac aggtctccta aatcactcca ttcttcatac
     1741 aggaatttgt ctagttctag tatcctccgg tatgtctttt ccgcttcatc atacttttcc
     1801 attcgatagt gaaaatgcct cgccaatttt atcaggggca acgtcgactg atcatccaac
     1861 tgataggcct gcttgtacgc gttctcagcc tcctcatacc tgtctagatt ctcgtataca
     1921 tttcctaagc caagccagga gtcaaatagc gacccattcc gttggatggc gttcagaaag
     1981 gccttttcag cctccagtgt ccttttcagg ttataataaa ctactcctaa
//
LOCUS       DATGFG010000126         3933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1460347, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000126 DATGFG010000000
VERSION     DATGFG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460347"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1823
                     /locus_tag="VKQ52_02350"
     CDS             147..1823
                     /locus_tag="VKQ52_02350"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HLZ86047.1"
                     /translation="MGKDNFVIGVDYGTDSVRSLLVNAADGSEIASAVFFYPRWKDGL
                     YCNPAENIWRQHPKDYIEGLEHTIKACLQQAGPAAAACVRAISVDTTGSTPVAVDETG
                     TPLGLLPAFENNPNALFVLWKDHSSVKEAAAINAHAATFDVNYLQFVGGIYSSEWFWA
                     KLLHILRMDESVRKACHSWVEHCDWIPFLLTGGRHAAELKRGVCAAGHKALWAAEFNG
                     FPPDEFFSSLDPLLKGFTTRLSKTTYTSDKPAGRLSPEWAGRLGLPADVIVGVGAFDA
                     HMGAVGGQIEPYHLSKVMGTSTCDMLVAPLKDVEGLLIKGICGQVPGSVIPGMMGMEA
                     GQSAFGDAYAWFRNLLAWPLQLLNIPGADPATSSAVTDAIIDKIIPELSRQAANLSPE
                     PGGELALDWFNGRRTPDANQLLKGAITGLHLASDAPRVFRSIVEATCFGARTIVDRFI
                     SEGVPVKGLIGLGGVAKKSPFIMQMMADVMNRPLRIHSSEQTCALGAAMFAATAAGLY
                     PRVEEAMAAIGQGFDATFTPDPARAAIYELRLRQYQQLGQFVEHTTKDYE"
     gene            1816..2514
                     /locus_tag="VKQ52_02355"
     CDS             1816..2514
                     /locus_tag="VKQ52_02355"
                     /inference="COORDINATES: protein motif:HMM:NF006047.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HLZ86048.1"
                     /translation="MSKYNDIQEAAYEANRQLPKLGLVLFTFGNVSAADRDLGVFAIK
                     PSGVPYSDLTPDKMVIVDFEGKTVKGKLRPSSDTLTHAVLYKHWEKIGGIAHTHSTYA
                     TAWAQSQRDIPIFGTTHADHNTVDIPCAPPMTDEMILGDYEYQTGFQILNAFRDKGLS
                     YEEVEMVLIGNHAPFTWGKTAEKAVYNSAVLESVAQMALLTEQIRKDAPRLKDSLIKK
                     HYERKHGPHSYYGQ"
     gene            2571..>3933
                     /gene="araA"
                     /locus_tag="VKQ52_02360"
     CDS             2571..>3933
                     /gene="araA"
                     /locus_tag="VKQ52_02360"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF002795.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HLZ86049.1"
                     /translation="MDLKKFEVWFVTGSQHLYGEETLKQVAAHSQTIAKALNDTPQIP
                     VKVVFRPTVKTPEEIYHTCLEANTTQNCIGIIAWMHTFSPAKMWIGGLRTLQKPLVHL
                     HTQFNRDIPWGEIDMDFMNLNQSAHGDREFGFMMSRMRLDRKVVTGHWQDPDTLARLG
                     AWTRAAAGWHDWQGAKFVRFGDNMRQVAVTDGDKVEAEMQFGYSVNTWGVGDLVASIN
                     ATPDSAVDTLVKEYEEKYTLADTLRKGKAQHNSLREAAKIELGIKTFLEQGGFKGFTD
                     TFEDLHGMVQLPGIAAQRLMAAGYGFAGEGDWKTAALVRAMKVMGSGLPGGNSFMEDY
                     TYHFEPGNNLVLGAHMLEICESIANGPVSCEIHPLGIGGKADPVRLVFNSAAGPALNA
                     SIVDMGNRFRLLVNEVEAVAPTHPLPKLPVARVLWKPYPDMTTGCTAWIIAGGAHHTG
                     YSQN"
BASE COUNT          945 a         1432 c          911 g          645 t
ORIGIN      
        1 catcccgcag aattacaacc ttttcgacgc accacccact ctcacagaaa caaatcccac
       61 ccccaacaaa tattttttaa aaaacaagaa attaaatgtt attttgacaa gtcttcacag
      121 aaaaaagacc atctccaacc agccatatgg gaaaagataa ctttgtcata ggtgtggatt
      181 acggcacgga ctccgtccga tccctcctcg taaacgccgc cgacggcagc gagatcgcct
      241 ccgccgtctt tttttacccc cgctggaagg acgggctcta ttgcaaccca gcggagaaca
      301 tctggcgaca gcaccccaag gattatatcg aaggcctcga acatacgata aaagcctgtc
      361 tgcaacaagc cggacccgcc gcggccgctt gcgtaagggc catctccgtg gacaccaccg
      421 gctcgacacc cgtggccgtc gacgagacag gtacccccct gggcctgctg cccgccttcg
      481 aaaacaaccc gaacgccctc ttcgtcctct ggaaagacca tagctccgta aaagaggccg
      541 ccgccatcaa cgcccacgcc gccaccttcg acgtcaacta cctgcagttc gtcggcggca
      601 tctattcctc ggaatggttc tgggccaagc tgctgcatat ccttcgaatg gacgaatccg
      661 ttagaaaagc ctgccactcc tgggtggaac actgtgactg gatccccttc ctcctcacgg
      721 gcggccgcca cgccgcagag ctgaaacgcg gcgtctgcgc agccggccac aaagccctct
      781 gggcagcaga attcaatgga ttccctcctg acgaattctt ctcctccctc gacccgctgc
      841 taaaaggctt caccacccgg ctgtccaaaa cgacgtatac ttccgacaaa cccgctggcc
      901 gcctgtcccc cgaatgggcc ggacgcctgg gtctccctgc cgatgtcatc gtcggcgtcg
      961 gcgcattcga cgcgcatatg ggcgccgtag gcggacagat cgaaccctac cacctcagta
     1021 aggtcatggg cacctccacc tgcgacatgc tcgtcgcccc cctcaaagac gtagaaggcc
     1081 tcctcataaa aggcatctgc ggccaggtgc cgggctccgt catcccgggc atgatgggca
     1141 tggaagccgg ccagtccgcc ttcggcgacg cctacgcctg gttccgtaac ctcctcgcct
     1201 ggcccctgca actcctgaac atccccggcg ccgatccggc cacctcatcc gccgtcaccg
     1261 acgccattat agataagatc atccccgaac taagccgcca ggccgccaac ctctcccccg
     1321 aacccggcgg agaactggcc ctcgactggt tcaacggccg gcgcaccccc gacgccaacc
     1381 aactgctgaa aggcgccatc accggcctcc acctggcgag cgacgccccc cgcgtattcc
     1441 gctccatcgt ggaagccacc tgcttcggcg cccgcaccat cgtcgatcgc ttcatcagcg
     1501 aaggcgtccc cgtaaaaggc ctcatcggcc tcggcggcgt agcaaaaaaa tcgcccttca
     1561 tcatgcagat gatggccgac gtcatgaacc ggcccctccg catccactcc tccgaacaaa
     1621 cctgcgccct cggcgcagcc atgttcgccg ccaccgccgc cggcctctac cccagggtag
     1681 aagaagcaat ggccgccatc ggccagggct tcgacgccac cttcaccccc gatccggccc
     1741 gcgccgccat ctatgagctg cgcctccgcc aataccagca actcggacaa ttcgtagaac
     1801 atacaacaaa agactatgag taagtacaac gatatacagg aagcagccta tgaggctaat
     1861 cgccagcttc ccaaactggg cctcgtcctc ttcaccttcg gcaacgtcag cgccgccgac
     1921 cgcgacctgg gcgtcttcgc cataaaacca agcggcgttc cctatagcga cctcaccccg
     1981 gataagatgg tcatcgtcga cttcgagggc aagaccgtca agggtaaact ccgcccatcc
     2041 tccgataccc tcacccacgc cgtcctctac aaacactggg aaaagatcgg gggcatcgcc
     2101 cacacccatt ccacctacgc caccgcctgg gcccaatccc aacgtgacat ccccatcttc
     2161 ggcaccaccc acgccgacca caatacggtc gacatcccct gcgccccgcc gatgacggac
     2221 gagatgatcc tcggcgacta cgaataccaa accggcttcc agatcctgaa cgccttccgc
     2281 gacaaaggcc tcagctacga ggaagtagaa atggtcctca tcggtaacca cgcccccttc
     2341 acctggggga aaaccgccga aaaagccgtc tataacagcg ccgtcctcga atcggtcgcc
     2401 caaatggccc tcctgaccga gcagatccgc aaagacgccc ccaggctgaa agattccctg
     2461 ataaaaaaac attacgaaag gaaacacggc ccacactctt attacggaca atagctgata
     2521 atcttccacc ccaagcacac catcaaaaaa gtacaaccat taaaaaagat atggacttaa
     2581 aaaaattcga ggtctggttc gtcaccggca gccagcacct ctacggagaa gaaacactaa
     2641 aacaggtggc cgcacactcc caaaccatcg cgaaagccct gaacgacacc ccacagatcc
     2701 cggtaaaggt cgtcttccgc ccaaccgtaa aaaccccgga agagatctac cacacctgcc
     2761 tcgaagccaa tactacccaa aactgcatcg gcatcatcgc ctggatgcac accttctccc
     2821 ccgccaaaat gtggatcggc ggcctccgca ccctccaaaa accccttgtc cacctgcaca
     2881 cccagttcaa ccgcgatatc ccctggggcg agatcgacat ggacttcatg aatctcaacc
     2941 aaagcgccca tggcgaccgc gaattcgggt tcatgatgtc ccggatgcgc ctcgatcgta
     3001 aagtagtcac gggccattgg caggatcccg acaccctcgc ccgcctcggc gcctggaccc
     3061 gcgccgccgc cggctggcac gactggcagg gcgctaaatt cgtccgcttc ggcgacaaca
     3121 tgcgccaggt agccgtcacc gatggcgata aagtagaagc cgaaatgcaa ttcggctatt
     3181 ccgtcaacac ctggggcgta ggagacctcg tcgcctccat caacgccacc cccgacagcg
     3241 ccgtcgatac cctcgtaaaa gaatacgaag agaaatatac gcttgccgat accctccgca
     3301 aaggcaaggc gcaacacaac tcactccgcg aagccgcaaa gatcgaactg ggaataaaga
     3361 ccttcctcga acagggcggc ttcaaaggat ttaccgacac cttcgaagac cttcacggca
     3421 tggtccagtt gcccggcatc gcagcccaac gcctcatggc cgccgggtac ggcttcgccg
     3481 gtgagggcga ctggaaaaca gctgccctcg tccgcgccat gaaagtaatg ggcagcggac
     3541 tacccggcgg caactccttc atggaagact atacctacca cttcgaaccg ggaaataacc
     3601 tggtcctcgg cgcccatatg ctcgagatct gcgaatccat cgccaacggc cccgtctcct
     3661 gcgagatcca ccccctcggc atcggcggca aagccgatcc ggtcagactg gtcttcaact
     3721 ccgccgccgg cccggccctc aacgcctcca tcgtcgacat gggcaaccgc ttccgcctcc
     3781 tcgtaaacga agtagaagcc gtagcgccaa cccacccact gccaaaactc cccgtcgccc
     3841 gcgtcctctg gaaaccctac cccgacatga ccacaggctg cacagcctgg atcatcgccg
     3901 gcggcgccca tcacaccgga tacagccaga acc
//
LOCUS       DATGFG010000127         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_773933, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000127 DATGFG010000000
VERSION     DATGFG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773933"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2605
                     /locus_tag="VKQ52_02365"
     CDS             <1..2605
                     /locus_tag="VKQ52_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HLZ86050.1"
                     /translation="PVKLQYYQLSMMDVARAVKANNNDVGGRKLDRGDMSYIIRGLGY
                     VKSREDLENIAVGNNKGFPIRVKDLGSVQMGGDLRLGIFDQNGQGEVVGGIVVMRYGE
                     NADKVIEAVKKKMADVQRGLPAGVAFRVAYDRSELIKAAVDNVKGKLVEEMIIVSVIV
                     LLFLLDLRSALSILIQIPITIATSFILLNAFGLSSNIMSLTGIALAIGVIVDNGIIMS
                     ENAYRHLAEEQNALPGGGSLPGATRMEVIRRACIQVSRGVFFSTVIIITSFLPVFLLT
                     GQEGKLFHPLAYTKTFILLVDAVLVVTLAPVLISFFMRGRFRGEGANPVNRVLERIYE
                     RVVRAGMRVRWVTLGLNVLALAVCVPLVLGLGREFMPPLDEGTILFMPVSLPDISNAE
                     IKRLLQVQDKIIASQPEVSNVLGKAGRASTATDNSPISMVETIVLLKPKAQWRPGVTR
                     DSIIRELNSKLQIPGVTNGWTQPIINRINMLTTGIRTDVGVKVYGQNLDTINVLAQQI
                     RTALNGIPGVKDLYVDPITGGKYLDIVIKREEIGRYGLTVDDVNSIVESALGGMKLTT
                     TVEGRQRFSVNARYGQDFRDNLTALRRLQVPTATMGPVPLEAVADIRLAEGPPMINSE
                     NAALRGTVLFNVRDRDLAGTVKEAQERLDKSLAGLPKGYYIEWSGEYENLIRSERTLT
                     WVLPVVLLIIFGAMYFAFRSAREAFFSLISIPFALIGGALAIYFWHVHLSVAVAVGFI
                     ALFGLAVETGIVMVIYLNDAMAQLVAAKGNSRETISRSHLREAVIAGAAKRLRPKLMT
                     VSVSLFALIPILWSNGVGSDVMKPIVLPMIGGVLTSATHILLVTPLIFLMTKEYELRK
                     YGRIEIMEAKH"
BASE COUNT          565 a          668 c          937 g          571 t
ORIGIN      
        1 ccccgtgaag ctccagtact accagttgtc gatgatggac gtcgcgcggg cggtgaaggc
       61 gaacaacaac gacgtaggag gcaggaaact tgaccgggga gatatgtcct atatcatccg
      121 tggactcgga tacgtgaaga gccgcgaaga cctggagaat attgccgtgg ggaataacaa
      181 aggcttcccc atacgggtaa aggatcttgg gtccgtgcag atggggggcg atctccggct
      241 gggcatcttt gaccagaatg gccaggggga ggtggtgggc ggcatcgtgg taatgcgcta
      301 cggggaaaat gcggacaagg tcatcgaggc cgtgaagaag aagatggcgg atgtgcagcg
      361 ggggctgccg gcaggggtgg ccttccgggt ggcctatgat cgcagcgaat tgatcaaggc
      421 ggcggtggac aatgtgaagg gaaagctggt ggaggagatg atcatcgtgt cggtgatcgt
      481 gctgctcttc ctgctggatc tgcgaagtgc gctaagtatc cttatccaga taccgatcac
      541 gattgcgacg agcttcatcc tgctaaatgc attcggattg tcctccaata ttatgtcgct
      601 gaccgggatc gcgctggcga tcggcgtgat cgtggacaat gggatcatca tgtcggagaa
      661 tgcgtaccgt cacctggcgg aggaacagaa cgcgttaccg ggcgggggct cattgccggg
      721 cgccacgcga atggaagtga tccggcgggc ctgtatccag gtgtccaggg gggtgttctt
      781 ttcgactgtc atcatcatca cctccttttt gccggtgttc ctgctgacgg gacaggaagg
      841 caagctgttt cacccgctgg cgtatacgaa gacttttatc ctgcttgtgg acgcggtgct
      901 ggtggtgacg ctggccccgg tgctgatctc cttctttatg cgggggcgct tccgggggga
      961 aggagccaac ccggtcaatc gggtgctgga gcggatatat gagcgagtgg ttcgtgcggg
     1021 tatgcgcgtt cgctgggtaa cgctgggtct aaacgtcctg gcgctggcgg tctgcgtgcc
     1081 gctggtgctg ggcctgggcc gggagttcat gccgccattg gacgaaggga ccatcctctt
     1141 tatgccggta agtctgccgg acatatccaa cgcggaaatt aagcgcctgc tgcaggtgca
     1201 ggataagatc atcgcgtcgc agccggaggt gtcgaacgtc ttgggaaagg cgggccgggc
     1261 cagcacggcg acggacaatt ccccgatcag catggtggag acgatcgtgc tgctgaaacc
     1321 aaaagcgcaa tggcggcccg gagtgaccag ggacagcatc atccgggagc tgaacagcaa
     1381 gttacagatc ccgggggtga caaatgggtg gacccagccg atcatcaacc ggatcaatat
     1441 gctgacgacg gggatacgga cggatgtagg agtgaaggtc tacggtcaga atctggatac
     1501 catcaacgtc cttgcacagc agatccggac cgccttaaac ggtattccgg gggtgaagga
     1561 cttatatgta gacccgatca cgggcggaaa gtatctggat attgtcataa agcgggaaga
     1621 gatcggccgg tacggattga ccgtggacga tgtgaatagt atcgtcgaga gcgctcttgg
     1681 cgggatgaag ctgacgacga cggtggaagg ccggcagcga ttctcggtga atgcgcggta
     1741 cgggcaggat ttccgggata acctgacggc gctcagacgg ttgcaggtac ctaccgcaac
     1801 gatgggcccg gtaccgctgg aagcggtggc ggacatccgc ctggcggaag ggccgccgat
     1861 gatcaattcg gagaacgcgg ctttgcgggg aacggtgctg ttcaatgtca gggaccgcga
     1921 cctggccgga accgtaaagg aggcgcagga gcggctggat aagtcgcttg ccggactgcc
     1981 gaaggggtat tacatcgagt ggagcgggga gtacgagaac ctgatccgca gcgagcggac
     2041 acttacctgg gtactgccgg tggtgctgtt gatcatcttc ggggcaatgt atttcgcgtt
     2101 ccggtcggca cgcgaggcat tcttttcatt gatctcgata ccctttgcgc tgattggcgg
     2161 agcactggcg atctatttct ggcatgttca cttgtccgtt gccgtagcgg tgggcttcat
     2221 cgcgctattc ggcctcgccg tagagacggg gatcgtgatg gtgatctacc tgaacgatgc
     2281 gatggcgcag ctggtggcgg ccaagggcaa ttcacgggaa acgatcagcc ggtctcacct
     2341 gcgggaggcg gtgatcgccg gagcggcgaa gcggcttcgg cccaagctga tgacagtgtc
     2401 ggtgtcgctc tttgcgctga tcccgatcct gtggagcaat ggggtaggca gcgacgtaat
     2461 gaagccgatc gtcctgccga tgatcggcgg tgtgctgacc tcggctacac atatcctgct
     2521 ggtgacgcca ctcatctttt tgatgacaaa ggagtacgaa ctgcgcaagt atggcaggat
     2581 tgaaataatg gaggcaaaac attaatacca tgacactaaa aataattttt tgtttggcgg
     2641 gcctgctggg cgtgctttct gcccgggcgc agacgacgat ggggctggac agcatttttt
     2701 cggtcatcgg ccgcagccat cccgcggtgc gttcttcgga t
//
LOCUS       DATGFG010000128         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1113587, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000128 DATGFG010000000
VERSION     DATGFG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113587"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VKQ52_02370"
     CDS             <1..245
                     /locus_tag="VKQ52_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86051.1"
                     /translation="NCQLGYNLGPAALSRLRIASMRIFVSAQNVFTITKYSGLDPEMT
                     TSANAASANEGPRALNIDWGTYPAARTITVGANINF"
     gene            263..1786
                     /locus_tag="VKQ52_02375"
     CDS             263..1786
                     /locus_tag="VKQ52_02375"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86052.1"
                     /translation="MRIKNLQLFLPVVLLLAGAGCKKILKQEVQGSYTPGNFFTSDAN
                     AQQAVNEAYKFLSFNSGATNAIWVLGDLASDDAIKGGSSIGDQPDFQAVNQFNILTTN
                     AAVEAVWKNYYNGVFACNVALDGLTGNKQVSAGMQQQGIAQAQFLRAYYYFILTTCYG
                     NIPLHLHVETAQEAQTPALPQDTIFARIEQDCIAAAAVLPVTVPSTQLGMATRGAALA
                     LLAKVYLFHNDLANHYALASQMAEQVEGMGYQLTNNYFDNFSAAAKDNTEAIFSVNHL
                     AGTLGIGNELNVWFAPRSPAPTNGYGFFLPTQSLVSNYESAPGGEPDPRLDYTIARVG
                     HNYFDLAYDPTWSSTGYNCKKMCQPLSEVPAATKNVGTVNYEALRLADILLIDAEALN
                     ESGQSAAALAPLNKVRARARNSYLYDNSLPGAPAVPAGLLPDVTVTDQTQLRDIIRRE
                     RRSELALEFHRWFDLIRYGKAYATAALSATAPNFNYDTNKWFPIPQSERDANPNLFK"
     gene            1812..>2400
                     /locus_tag="VKQ52_02380"
     CDS             1812..>2400
                     /locus_tag="VKQ52_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86053.1"
                     /translation="MRISNYILYLAFAGGLLAATASCKKNSGSPATYTTDKTVLNAVI
                     DSLTNVFNHSVEGTKPGTYSVGAREALDSAIGLAREVSSSNAFTQQQVNNAVNSLRNA
                     GVDFSNHLLQQVSAANLVAYWTFSGNAKDSSGNGHNGTLQTGWTGSSATTAVDGGTLP
                     VLVADRFDRAGMAYYFNNGATIDVPYSGALNPQSFT"
BASE COUNT          539 a          689 c          694 g          478 t
ORIGIN      
        1 agaactgcca attgggctat aacctgggac cggctgccct gagccggctc aggatcgctt
       61 cgatgcgcat cttcgtcagc gcgcagaacg tcttcaccat caccaaatac tcgggcctgg
      121 acccggagat gacgaccagc gccaatgcgg cctctgccaa tgagggaccc agggcgctga
      181 atatcgattg gggtacctac cccgccgcga gaacgatcac cgtgggcgca aacattaatt
      241 tctaacggat aactatattg tcatgcgaat aaaaaatctg caattgtttt taccggtggt
      301 actgctgttg gccggcgccg gttgcaagaa gatcctaaaa caggaggtgc agggcagcta
      361 tacacccggc aacttcttta cctccgatgc caatgcgcag caggccgtga acgaggccta
      421 taaatttctt tcgttcaatt cgggtgctac caatgccatc tgggtgctgg gtgatctggc
      481 ctcggatgat gccatcaagg gtggcagcag catcggggac cagcccgatt ttcaggcggt
      541 gaaccaattc aatatcctca cgacgaacgc ggcggtggaa gccgtctgga agaactatta
      601 caacggcgtc tttgcctgta acgtggccct cgacgggctg acgggcaata agcaggtgtc
      661 ggccggcatg cagcagcagg gcatcgccca ggcgcagttt ctccgggcct attattattt
      721 catcctcacc acctgttatg gcaatattcc cctgcacctc catgtagaga cggcacagga
      781 agcacagaca cccgccttac cgcaggacac gatctttgcc cggatcgaac aggattgcat
      841 tgccgccgct gccgtactgc cggttaccgt accctcgacc caactgggaa tggccaccag
      901 gggcgcggcg ttggccctgt tggcaaaggt ctacctgttt cacaacgatc tggcgaatca
      961 ttatgccctg gcgtcgcaga tggcggagca ggtggaggga atgggatatc agctgaccaa
     1021 taactatttc gacaatttca gcgccgcagc gaaggacaat acggaagcca tcttctccgt
     1081 caaccatctc gccggcacgc tgggcatcgg caacgagctg aacgtctggt tcgcgccccg
     1141 gtcgcccgcg cctaccaatg ggtacggatt cttcctgccg acccagagcc tggtgtctaa
     1201 ttatgagtcg gcgccgggtg gagaaccgga tccgcggctg gactatacca tcgccagggt
     1261 gggacataac tatttcgacc ttgcgtatga tcccacctgg agcagtacgg gatacaattg
     1321 taagaaaatg tgccagcccc tttcggaagt tccggcggcg accaagaatg tcggcacggt
     1381 gaattatgag gcgctccgcc tggcggatat cctgctgatc gatgcggagg cgttgaatga
     1441 gagcggccag tccgcggcgg cgctggcgcc cctgaacaag gtgagggcga gggccaggaa
     1501 ttcgtacctg tacgataaca gcctgcccgg cgctcctgct gtcccggcgg gtctgttgcc
     1561 ggatgtgacg gttaccgatc agacccaact gcgggatatc atccgcaggg aaagaaggtc
     1621 tgagctggcg ctggaattcc accggtggtt cgatctcatc cgctacggca aggcgtatgc
     1681 cacggcggcg ctttccgcca ccgcgccgaa tttcaactat gatactaaca aatggttccc
     1741 cattccgcaa agcgaaaggg acgccaatcc gaatcttttc aaataacggg ttcacaaaaa
     1801 acatccgaag tatgcgtatc tccaactata tactatacct ggcatttgcc gggggactgc
     1861 tggcggcgac ggcgtcctgt aagaaaaata gcggttcgcc cgcgacctat accaccgata
     1921 agacggtctt gaacgcagtg atcgattcat taaccaatgt cttcaatcat tccgtggagg
     1981 gcaccaagcc cgggacctat tccgtcggcg cccgcgaggc cctcgattcc gcgatagggc
     2041 ttgccaggga agtgagcagc agcaatgcct tcacccagca acaggtgaat aatgccgtga
     2101 acagcctccg gaatgccggg gtggacttca gcaatcactt gctgcagcag gtgtcggcgg
     2161 cgaacctggt ggcctactgg acctttagcg gcaatgcaaa ggactcttcc ggcaacgggc
     2221 ataacggtac actgcaaacg ggatggacgg gcagcagcgc gactacggcg gtggatgggg
     2281 gaacgcttcc cgtgctggtg gcagaccggt tcgacagggc ggggatggcg tattatttca
     2341 acaacggcgc gacgatcgat gtgccgtata gcggcgccct caatccccag agcttcacca
//
LOCUS       DATGFG010000129         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1688910, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000129 DATGFG010000000
VERSION     DATGFG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688910"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2034
                     /locus_tag="VKQ52_02385"
     CDS             <1..>2034
                     /locus_tag="VKQ52_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ86054.1"
                     /translation="NIFVRGVTSFGNTDPLYIIDGVPGGLHDLNANDIESLQVLKDAG
                     AASIYGVRGSNGVIIITTKRGKSGRSTISYDGYYGTQQPLPGNPFHLLNSQELANALW
                     KAEIQSGQVAANGFPTSQQYGNGATPVLPDYITPGGAHEGDASVNPALYNVDYSKGNI
                     YQITRANKQGTNWFAQLFKSAPIQSHTLSMSGGAEKSTYYFSLGYFNQQGTLLETYLK
                     RYSAKINTTFNVKKNIRVGENAYIFFKSNPQIAYFSENVINYDYREQPIIPVYDIKGN
                     YAGSNGPELGNSPNPVANQLRTANNRGYNWDIFGNVWGEVDFLKHFTVRTSFGGTIDN
                     NYGYQYTYHTYENAENNGSNGFLEFAGYNSSWTWTNTLNYTNTFGDHSLHLLGGTEAI
                     QYSGRNIFGSRINYFNDDPSLQVLSAGSPLGQVNGSNANRASLSSYFGKAEYAFKGRY
                     LLGATVRRDGASPFGPDKQYGVFPAFSGGWQISDEEFMKPVTFVNFLKLRGSWGKMGS
                     YANVPPSNSYTQYSSTPGNSNYDINGTSSGTVQGFYNSSIGSLVTGWEEDKITNIGLD
                     AVVLNNKLDLSLEYYQKKINGLLFQDQTSALIGGAAPPNVNIGDVKNNGFDFSAIYHG
                     AVNRDFKYNATLIFTTYKSDVVSIPGAGGYFESGNTRIGNFVRNSVGHTISAF"
BASE COUNT          507 a          494 c          549 g          484 t
ORIGIN      
        1 gcaatatttt tgtccggggc gttacctctt ttggcaatac cgatccgctg tatattattg
       61 acggagtgcc gggtggtctt catgacctca acgccaatga tatcgagtcc ctgcaagtat
      121 tgaaggatgc gggcgccgcg tccatttacg gggttcgggg ttccaatggg gttatcatta
      181 tcaccacgaa acgggggaaa tcgggccgga gcacgatcag ctacgatggc tactatggca
      241 ctcagcagcc cttacccggc aaccctttcc atttgctgaa ttcccaggag ctggccaacg
      301 cactctggaa ggcagaaatc cagtcgggac aggtggctgc caatgggttt cccacttcac
      361 agcaatacgg gaacggggct acgccggtgt tgcctgatta tattacgcct ggtggggctc
      421 atgagggaga tgcatcggtg aatcccgccc tgtataatgt ggattacagc aaggggaaca
      481 tctaccagat caccagggcc aataagcagg gcaccaactg gttcgcgcag ttattcaaat
      541 cggcgccgat ccaaagccat accttgtcga tgagcggcgg ggctgaaaag tcgacttact
      601 atttctcgct ggggtatttc aaccagcagg gcacgctgct ggagacttat ctcaaaaggt
      661 attccgccaa gatcaatacc accttcaacg taaagaaaaa tatccgggtc ggggagaatg
      721 cctatatttt cttcaagagc aatccgcaga tcgcgtactt cagcgaaaat gtcatcaact
      781 atgattaccg ggagcagccg atcatcccgg tgtatgacat caagggcaac tatgcgggtt
      841 ccaatggtcc ggagttgggg aattcgccca atcctgtggc caatcagctg cgtacggcca
      901 ataacagggg atataactgg gatatctttg gaaatgtctg gggcgaggtt gattttctga
      961 aacattttac cgtcagaacc agctttggcg gaacgatcga caataactac gggtaccagt
     1021 acacttacca tacgtatgaa aatgccgaaa ataacggatc aaacgggttc ctggagttcg
     1081 caggctacaa cagcagctgg acctggacga acacgttgaa ttacacgaac accttcggcg
     1141 atcatagcct acatctcctg ggtggtacgg aggcaatcca gtattccgga aggaatatat
     1201 ttggcagccg tatcaactat ttcaatgacg atccttccct gcaggttctt tcggccggtt
     1261 cgcccctggg ccaggtgaac ggcagcaacg ccaaccgggc gtcgttgagc tcctatttcg
     1321 gaaaagcgga atatgccttt aaagggcgct accttttagg ggcgacggtc agaagggacg
     1381 gcgcttcgcc ttttggtccg gacaaacaat atggcgtttt cccggccttc tccggaggct
     1441 ggcaaatttc cgacgaggaa tttatgaagc cggttacttt tgtcaacttc ctgaagttgc
     1501 ggggcagctg gggaaagatg ggatcctatg cgaatgtgcc gcctagtaac tcttacacgc
     1561 agtactcttc gacgccgggt aattccaatt acgatattaa cggaacaagc agcggcacgg
     1621 tacagggttt ctacaattct tccatcggca gccttgtcac gggttgggag gaggataaga
     1681 tcaccaatat cggtttggat gccgtggtgc tgaataacaa actggatctg agcctcgaat
     1741 attatcagaa gaagatcaat ggtcttttgt tccaggatca gacttcggcg ctgataggcg
     1801 gcgcggcgcc gccgaatgtg aatatcggtg acgtaaaaaa caatggattt gacttttctg
     1861 cgatttatca tggagcagtg aaccgggact ttaaatacaa cgccaccctg atctttacca
     1921 cttacaagag tgatgtggta agcatacccg gggcgggtgg gtattttgaa tcggggaata
     1981 cgcggatcgg caattttgtc cggaattccg ttggccatac catcagcgcc tttt
//
LOCUS       DATGFG010000130         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1230434, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000130 DATGFG010000000
VERSION     DATGFG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230434"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2212
                     /locus_tag="VKQ52_02390"
     CDS             <1..2212
                     /locus_tag="VKQ52_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ86055.1"
                     /translation="SIEYNAGLDLLLVSTHTEGFYFLRRDHFRIDGWSPMVQDRMTRH
                     LFGPMALRNGKEILTDKFIFTPAGGLTPLKGSGPVWQRCLFVDTHDGVWAAQHTIPRK
                     LNGAMDPVAEFPALDANVVDYAEEDKGNLYCLTERSLWRLDSGVFSRVGIPPPGDEQQ
                     KNETMAFVGGHRLWIASTNGMVEYDPEKKAAHPVPELAGTHVRAIHVCRDGSVLIGTY
                     GEGYYYYFHQHFFRMPMDKNNFLITAHCFLEDRRGYIWIPCNKGLFKVPKADMDAWCM
                     DGNNQLYYYYYGRQDGLKTNEFNGGFNASGVITPEGFVSLLSMKGMVCFYTDSLGTDF
                     PQGSIDMVHLEIDGKSAARTDTINLSAGYNSLVLEISCPYLGNRNNLYLQYNLSGLSD
                     EWKEMPEDGMLNLSRLAPGTYTLRVRKVNGFGKNNYQYRQWNIIVPRYFYKTGWFLLL
                     AGLALLVLLIVLVQLRLKLIEKQKEIRIKAENLKDTVVRLEETIEKLQHSEQALLRTN
                     KQREKLISLVIHDLRSPLRFLTMLAGDLHDNQGGFSPEEMKDRTYWVKKGAQDIYNFS
                     EDFLLWVTSQKDNFKISKRLFFVRPLLQEIYDFYLEQVLQKGNRLSYEADETLQVYSD
                     PHLVITIIRNLTDNANKYTTGGDIRIEARRKGNELLIVVADTGRGMSPKQAAAFLGEG
                     NLDNVKSGSQLGHKFIVDLTRRLNGTLSIDTGEKTGTTVTLRIPMEPAADTGAA"
     gene            complement(2219..2485)
                     /locus_tag="VKQ52_02395"
     CDS             complement(2219..2485)
                     /locus_tag="VKQ52_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ86056.1"
                     /translation="MDSILKKTYTSYHRLNFLFKSIHGMTISEYIRNKRIEKSKEMIA
                     DNVKSISQIAYEIGYSSISNYILAFKKVYQITPGKYKKQIDSLA"
     gene            complement(2501..3142)
                     /locus_tag="VKQ52_02400"
     CDS             complement(2501..3142)
                     /locus_tag="VKQ52_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86057.1"
                     /translation="MKSQDPDQEKTIEALHWQVLSLPVGLRAAVLRSSADRLELPLPE
                     FNEKGYLLTFINYKGDLRIKAGRRPEGCEQPASPGMGLMSVADRPMVFRAGSPISFAA
                     LVLFIPAEWVDLFLGSNPQFGKIEQLLGEEPNILTCRLGRRQLSLLSSALTVLQDKHQ
                     HLLSTQNDALALLEYFLCNRHRSLLGVNPTSDIDNEDDLSLIREVESYISEYY"
     gene            complement(3189..>3696)
                     /locus_tag="VKQ52_02405"
     CDS             complement(3189..>3696)
                     /locus_tag="VKQ52_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ86058.1"
                     /translation="ATLVWPITFKYRLILNDFMTEIVQHPFRELFTIRNISVLIEYLL
                     QQFFVMDKLSKEGNALSIQDTHSLSRVEQHLCLHYKKEFPGIEKLSRISAMSPTSLKT
                     KFKKYYGETLFGYYQKNKLEHARKLLDSKIPVKVVATEIGYSNPSHFTLAFKKEYGFS
                     PWHYLNPH"
BASE COUNT          848 a          962 c         1036 g          850 t
ORIGIN      
        1 ctctattgaa tacaacgccg gtctcgacct cctcctggtc tccactcata cggaaggatt
       61 ttattttctt cgccgggatc atttccggat cgacggctgg tccccaatgg tacaggatag
      121 gatgacgcgg cacctttttg gtcccatggc gctgcgcaac ggaaaggaga tccttaccga
      181 taaattcatt ttcacgccgg ccggcgggct tacgccgctg aagggaagcg gtcctgtctg
      241 gcagcgctgt ttgtttgtcg atacccatga cggggtctgg gcggcccaac ataccattcc
      301 acggaaacta aacggcgcga tggatccggt ggcggaattc cccgccctcg atgccaatgt
      361 cgtcgactat gccgaagagg acaagggcaa tttgtattgt cttaccgagc gatcgttatg
      421 gcgattggat tccggggtct tcagtcgggt gggtatcccg ccccccgggg atgaacagca
      481 gaagaatgaa accatggcct ttgtcggcgg ccaccggttg tggatcgctt cgacaaacgg
      541 gatggtcgaa tacgatccgg aaaagaaagc ggcgcatccc gtcccggaac tggcgggcac
      601 gcatgtccgg gccatccatg tctgccggga tggatcggtg cttatcggaa cgtacggaga
      661 aggctattac tattattttc atcagcattt cttccggatg ccgatggata agaacaactt
      721 cctgatcacg gcgcattgtt tcctcgaaga tcgccgggga tatatctgga tcccctgtaa
      781 caagggcctg ttcaaggttc ccaaggccga tatggacgcc tggtgcatgg atgggaacaa
      841 ccagttgtac tactactatt atggacgcca ggacgggtta aagaccaatg aattcaacgg
      901 cggattcaac gcgagtggag tgatcacccc ggaaggattc gtttcgctcc tgtccatgaa
      961 aggtatggtc tgtttctata ccgactccct cgggaccgat tttccccagg gttccatcga
     1021 tatggtccat ctcgagatcg acggcaagtc cgcggcgcgg accgatacga tcaacctgtc
     1081 ggccggttat aacagcctcg tactggagat ctcctgtccc tatctcggca accggaacaa
     1141 tctctatctc caatacaatt tgtccgggct cagcgacgag tggaaggaga tgcccgagga
     1201 cggaatgctc aacctgagcc ggctggcgcc cgggacctat accctgcggg tccgcaaggt
     1261 gaacgggttt ggcaagaaca attaccagta ccgccaatgg aacatcattg tcccgcgcta
     1321 tttctacaag acgggctggt tcctgctcct ggcagggctg gccctgctgg tcctgctcat
     1381 cgtgctggtg cagctgcggc tgaagctgat cgaaaaacag aaggagatca ggataaaagc
     1441 ggagaatttg aaggacaccg ttgtccggct cgaagagacc atcgagaaat tacaacactc
     1501 cgagcaggcc ctgctccgga ccaataaaca gcgtgaaaaa ctgatctcgc ttgtcatcca
     1561 tgacctgcgt tcccccctgc gcttcctgac gatgctcgcc ggcgacctgc atgataacca
     1621 gggcggtttt tcgccggagg aaatgaagga tcgcacctat tgggtaaaaa aaggcgcaca
     1681 ggatatttat aatttctccg aggatttcct gctttgggtg accagtcaga aggataattt
     1741 caagatcagc aaacgtctct tcttcgtccg gccgctcctg caggagatct atgattttta
     1801 cctggaacag gtgttgcaga aagggaaccg gctttcgtat gaagccgatg agacattaca
     1861 ggtctactcc gatccccacc tcgtgatcac catcatccgc aacctgacgg acaatgccaa
     1921 caaatacacc accggggggg atatccgcat cgaggcaaga cggaaaggga atgagctgct
     1981 gatcgtcgtt gccgacaccg gccggggtat gagcccgaag caggccgcgg ccttcctggg
     2041 cgaaggcaac ctggacaatg tcaaaagcgg cagtcaactg gggcataagt tcatcgtcga
     2101 tcttacccgg cggctgaacg gaacgctttc catcgacacc ggggaaaaga ccggcaccac
     2161 ggtgaccctg cgtatcccga tggaaccggc cgccgatacc ggcgccgcct gatgcggctc
     2221 aggccaggct gtcgatctgt ttcttatact tgcccggcgt gatctggtac actttcttaa
     2281 aggcgaggat ataattactg atgctcgaat accctatctc ataagcgatc tggctgatcg
     2341 acttgacgtt gtccgcgatc atctcctttg acttctcgat ccttttgttc cggatatatt
     2401 ccgagatggt catcccatgg atggacttga agaggaaatt cagccggtgg tagctggtgt
     2461 aggtcttctt caggatggaa tccactgtaa aggtatcgga tcaatagtat tccgaaatat
     2521 aggactccac ctcccggata agactcaggt cgtcctcatt gtcgatgtcg gaggttggat
     2581 tgacgcccag caggctccgg tgccggttac aaagaaaata ttccagcagg gccagcgcat
     2641 cgttctgcgt gctcagcaga tgctggtgtt tgtcctgcag gacggtcaat gccgaagaga
     2701 gcagggataa ctgccttctg cccaggcggc aggtgaggat attgggctct tcccccagca
     2761 gctgttcgat cttcccgaac tgggggttac tgccaaggaa cagatcgacc cattccgccg
     2821 gaatgaacag gacaagggcg gcaaaagaga tggggctgcc tgcccggaat accatcggcc
     2881 ggtcggcaac gctcataaga cccatgccgg gactggccgg ttgttcgcag ccctcgggcc
     2941 ggcggcccgc cttgatacgg agatcgcctt tgtaattgat gaacgtgaga agatagcctt
     3001 tttcgttgaa ctcgggaagg ggaagctcca atcggtctgc cgatgatcgc agtactgcgg
     3061 cccgcaggcc aaccggtagg gacaatacct gccaatgcaa agcctcgatg gtcttttcct
     3121 ggtcaggatc ctgggatttc atagtcatgt atttcggggg ttagagtgaa gggtcagttg
     3181 gagaggtctc aatgggggtt gagataatgc cagggggaga acccgtattc cttcttgaag
     3241 gccagcgtga aatgggaagg attggaatag ccgatctcag tggctaccac ttttaccggg
     3301 atcttgctat ccagtagttt ccgggcatgt tccagtttgt tcttctggta atatccaaac
     3361 agggtctcac cgtaatattt cttgaatttg gtcttgagac tcgtggggct cattgccgaa
     3421 atgcgtgaca gtttctcgat accggggaat tcttttttat aatgcaggca aagatgctgc
     3481 tcgacacggg aaaggctatg ggtgtcctgg atactgagcg cattaccctc cttgcttagt
     3541 ttatccatta caaagaactg ctgcagaagg tattcgatca ggaccgagat attgcggata
     3601 gtaaacagct cccggaaagg gtgctggacg atctccgtca tgaaatcgtt caggatcagc
     3661 ctgtatttaa acgtgatcgg ccagacaagc gttgcg
//
LOCUS       DATGFG010000131         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1575659, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000131 DATGFG010000000
VERSION     DATGFG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575659"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2516
                     /locus_tag="VKQ52_02410"
     CDS             <1..2516
                     /locus_tag="VKQ52_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86059.1"
                     /translation="GAVQVTIDPANPQIVYADLWAGRQGPWENGAWNGPNSGLFKSTD
                     GGTTWRQLTQGLPTPAQGLSRIGFCIAPSDPSRLYATVEAGANGGIYRSDDAGESWKK
                     MNSDQRFWGRGDDFAEVKADPKNKDIVYTADVVVWKSTDGGQSWDAFRGAPGGDDYHR
                     LWINPDHPEIMLIACDQGAIVTVNGGQTFSSWYNQPTAQFYHVSTDNAFPYNVYGGQQ
                     ESGSIGIASRGNDGEVTFREWHPVGVEEYGYIAADPLDPDIIYGGKITRFDKRTGQVQ
                     NIAPEAIRSGKYRFLRTAPVLFSPTDAHTLFFAGNVLFKTSDGGHSWQVISPDLSRAA
                     WEIPASVGIYSTDALKKMPRRGVIYTVAPSPVDGQTIWAGTDDGLIHVTHDGGKTWNN
                     VTPPGIPDWTKISLIDAGHSDAATAYAAVNGIRVDDMHPHIYKTHDGGRTWTAIVNGL
                     PDDPINVVREDPHHKGLLMAGSERCVYISFDDGAGWQPLRLNMPATSIRDLVFKDADV
                     VVGTHGRSFWILDDIAPLRQLSAAARADAVLYKPEPAIRVRWDMNTDTPLPQEEPAGE
                     NPPDGAIIDYYLQANATTPVTLDIMDATGRLVRHYSSADTLYTIPDVNIPQYWIRPQQ
                     VLSAHAGPHRFCWDLHYQPLNVPASYPISAVYGNTAPVATSPWVMPGAYTLKLSVNGK
                     AYTQQLMVRMDPRVKTPASALQQQYTLAMEAYKGRQRAMTALERVRRLRTHIATALSS
                     ASGDRTTALQQLDAKAAALEGNARRGRGMPAAADNGAKSFSQLQNDYAAVFGILEEAD
                     LPPTVQAEAALQATAQAAHSAETAFTALQHEAVTLNIAL"
     gene            2513..>2722
                     /locus_tag="VKQ52_02415"
     CDS             2513..>2722
                     /locus_tag="VKQ52_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86060.1"
                     /translation="MNYRLTVLALLSPVFLLAQKKPVGPAPASPDSLFYAPLKYRLIG
                     PFRGGRADAVSGSLTNKHTFYFGATG"
BASE COUNT          546 a          874 c          811 g          491 t
ORIGIN      
        1 ccggcgccgt gcaggtgacc atcgacccgg cgaaccccca gatcgtgtat gccgatctct
       61 gggcaggacg ccagggtccc tgggagaacg gcgcctggaa cggccccaac agcgggctgt
      121 tcaaatccac ggacggggga accacgtggc ggcagctgac gcagggactt cccaccccgg
      181 cgcagggcct cagccgtatc ggcttctgta tcgccccctc cgatccgtcg cggttatacg
      241 ccaccgttga agcgggcgcc aatggcggca tctatcgcag cgatgacgca ggcgaatcct
      301 ggaaaaagat gaacagcgac cagcggttct ggggccgtgg ggacgatttt gccgaggtaa
      361 aggcggaccc gaagaataag gatatcgtct atacggccga cgtggtcgtc tggaagtcca
      421 cggacggcgg ccagtcctgg gatgccttcc gcggtgcacc cggcggggac gattatcacc
      481 gcctctggat caatcccgat cacccggaga tcatgctcat cgcctgcgac cagggcgcga
      541 tcgttaccgt caacggcgga cagacctttt cctcctggta taaccagcct acggcccagt
      601 tctatcatgt gagcacggac aacgcctttc cctataatgt atatggcggc cagcaggaga
      661 gcggctccat cggtatcgcc tcccggggta atgacggtga agtaaccttc cgggaatggc
      721 atcccgtggg agtggaagaa tatggctata tcgccgcgga cccccttgac ccggatatca
      781 tttacggcgg gaagatcacc cgctttgaca aacgcacggg gcaggtgcag aatatcgcac
      841 ccgaggcgat acgcagcggc aagtatcgct tcctgcggac ggcgccggtg ctgttctcac
      901 cgaccgatgc gcatacgctt tttttcgcgg ggaacgtgct tttcaaaaca agtgacggcg
      961 gccattcctg gcaggtgatc agccccgacc tgagccgcgc cgcctgggag atcccggcaa
     1021 gcgtcggcat ctacagcacc gacgcgctga agaagatgcc ccgccggggt gtcatctata
     1081 cggtggcgcc ttcccccgtg gatgggcaga ccatctgggc gggaaccgat gacgggctga
     1141 tccacgtcac ccatgacggc ggaaagacct ggaacaatgt cacgcccccc gggatacccg
     1201 actggacgaa gatcagcctg atcgacgccg gccacagcga cgcggctacg gcctatgccg
     1261 ccgtcaatgg tatccgggtg gatgatatgc acccgcatat ctataagacc catgatggcg
     1321 gacgcacctg gacggccatc gtcaacggtc tgcccgacga tcccatcaac gtggtaagag
     1381 aggacccgca ccacaagggg ctgctgatgg cgggctcgga gcgctgtgtc tatatctctt
     1441 ttgacgatgg cgccggctgg cagccgctgc ggttgaatat gccggcgacc tctatccgcg
     1501 acctggtctt caaggatgcg gatgtggtgg tggggacgca tggccgctcg ttctggatcc
     1561 tcgatgatat cgctccgctg cggcagttgt ctgctgccgc cagggccgat gcggtgctgt
     1621 acaaacccga accggcgatc cgcgtccgct gggatatgaa taccgacacc ccgctgccgc
     1681 aggaagagcc cgcgggtgag aacccgccgg atggcgccat catcgattat tatctgcagg
     1741 cgaacgccac gacgccggtg acgctggata tcatggatgc caccggcaga ctggtccggc
     1801 attattccag cgccgacacc ctgtatacca ttcccgatgt caatatcccc caatactgga
     1861 tccggccgca acaagtactg tcggcccacg ccggcccgca tcgcttttgc tgggacctcc
     1921 actaccagcc gctgaacgtg ccggcctcct atccgatcag tgcggtgtac ggcaataccg
     1981 ctcccgtagc tacttccccc tgggtaatgc ccggcgccta taccctcaaa ctttctgtca
     2041 acggaaaggc atatacgcag caattgatgg tgcggatgga tccgcgggtg aagacgccgg
     2101 cgtcggccct gcaacagcaa tatactctcg cgatggaggc ctataaggga agacagcgcg
     2161 ccatgacggc cctggaaagg gtccgccggc tgaggacgca tatcgccacg gccctgtctt
     2221 cggcatccgg cgaccggacg acggcgctgc aacagctcga tgctaaagcc gcggcactcg
     2281 aaggcaatgc ccgccggggc aggggaatgc ctgcggcggc ggacaacggt gccaaaagct
     2341 tctcccaatt gcaaaacgac tatgccgccg tcttcggcat cctggaagag gcggatctcc
     2401 cgcctaccgt tcaggctgaa gcggcattac aggcgaccgc acaggcggcg catagcgccg
     2461 agaccgcgtt caccgcactg caacacgaag ccgtcacttt aaacatcgca ttatgaacta
     2521 tcgtcttacc gtcctcgcac tcctgtcgcc ggtgtttctc cttgcacaga aaaagcccgt
     2581 tggccccgca ccggcttccc ccgattcgct tttttacgcc ccgttgaaat accggctcat
     2641 cggccccttc cggggtggcc gggccgacgc cgtatcgggc agtcttacca ataaacatac
     2701 tttttacttt ggcgccacgg gc
//
LOCUS       DATGFG010000132         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_201200, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000132 DATGFG010000000
VERSION     DATGFG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201200"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VKQ52_02420"
     CDS             complement(<1..882)
                     /locus_tag="VKQ52_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86061.1"
                     /translation="MIKNWLLTAIRNMRKNRTASAINIFGLTVGLSSCLLIALFIRHE
                     LSYDDFEVKGPRIARVIMEYRFNGGGDIQRGNFTSTKVATTFKRVFPEVESAVRMTER
                     PRIISYKDKLFTEDRFMFADSSFFLAFSMPLIRGDAVTALSGPKKVVLTESTARRYFG
                     AEEPLGKILRIGTDSVDYEVTGLMADCPSNSQFKFDFLASFSSLYANQDETYWDANYG
                     TFLLLRNPSDLARLQAKIGGFMVKEMQGQGAYVNFLLEPFMRIHLYSEFVGFEPGTSI
                     VYIYILAGVALLILVIAG"
     gene            1308..2486
                     /locus_tag="VKQ52_02425"
     CDS             1308..2486
                     /locus_tag="VKQ52_02425"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ86062.1"
                     /translation="MKLFVPTTVLPILCLLMTRPCPAQISTGPANPGTAADVSLAGSS
                     FTWNTPNGALSAGGSSATTAAILTAYTGTTDYLQLTNFGFAIPTGSTIDGIQANVTHM
                     ASGLSLTIGILTVTGSVTDNIVQLVGPGVTSVNKASGASWPTSNTVASYGSSSDGWGT
                     TVWTAAMVNSPGFGVAVSANMNGNTLLGVDLIPGAAIDLVTINITYSLPVTLPVQLDQ
                     WAVVRQGDVDVLRWQAGADAIPASTQASGAPAEFIVERSANSSDWSDLATIAASSGLR
                     LYTYTDEEPFANGPTYYRLRLHSEGRPDSWSTIQVLAAKQVQHPVINVYPNPFHNTVN
                     VSAADPFTRLTLKDRQGATLWVKEFSGGANKAQLPAAGLPQGLYFLTIDGATYKMMKE
                     "
BASE COUNT          568 a          840 c          733 g          597 t
ORIGIN      
        1 gccagcgatg acgaggatca gcaacgccac ccccgccaat atatagatgt agacgatact
       61 cgtccccggc tcgaatccca cgaactccga atagagatgg atacgcatga acggctcgag
      121 caggaaattc acataggccc cctgtccctg catctctttt accatgaacc caccgatctt
      181 ggcctgcaac cgtgccaggt cggacgggtt gcgcaacagc aggaaggtgc cgtaattggc
      241 gtcccagtag gtctcgtcct ggttcgcgta cagggaggag aaggaggcca ggaagtcgaa
      301 cttgaactgc gaattcgacg ggcagtccgc catcagtccc gtcacctcgt aatcgacgct
      361 gtccgtaccg atgcgtaaga tcttccccag gggctcctcc gccccaaaat acctccgcgc
      421 cgtggactcc gtcagcacca cttttttcgg tcccgacagg gccgtgaccg catctccccg
      481 gatcagcggc atactgaagg ccaggaagaa actcgagtcg gcgaacatga accggtcttc
      541 ggtaaagagc ttatccttat aggagatgat ccgcggccgt tcggtcatcc gcaccgccga
      601 ctccacctcc gggaacaccc gtttaaaggt ggtcgccacc ttggtgctgg taaaattgcc
      661 gcgctggata tcgccgcccc cgttgaaccg gtactccatg atcacccggg cgatgcgggg
      721 tccttttacc tcgaaatcgt catagctgag ctcgtggcgg ataaaaaggg cgatgagcag
      781 gcagctggac agccccaccg tcagcccgaa aatgttgata gcagaggcgg tgcggttctt
      841 ccgcatattg cggatagcag tcaaaagcca gtttttgatc ataatccaaa cagttttagc
      901 aggtttttgt agtttttatt ctaatgctgt gtagtattta atttagtcga atgccgctag
      961 caaagggtag gtgtttaccc ctaaatcgta tttctacaag cgcgcgcaat tttgattcca
     1021 gcaaaaatcg tcgtccttgc agtggaattc atacagacta tcgtttagac catcctcagt
     1081 agcatctctt aaaaaactgg cagccatcca ggactggtaa caatcccagt agtagtagta
     1141 gcaatcccag ggctcaactc ttaccggcgc ccctgaccag gcccggccgt tccttataac
     1201 aaccgtctca ccggcctgtc cgcttacccg accaggtgat tcatttattc cggggctttc
     1261 ccctggacgc atgacccgta tctgaagtta cccattaatc catatatatg aaacttttcg
     1321 taccaaccac tgtgctcccg atcctatgtc tgctgatgac cagaccgtgc cctgcccaga
     1381 tcagtaccgg tcctgccaat cccgggacgg cggccgatgt ttctcttgcc ggatcttcgt
     1441 tcacctggaa taccccgaat ggtgcgctgt cagcgggcgg atcttccgcc accaccgcag
     1501 ccatcctgac ggcgtatacc gggacgaccg actatctgca actcacgaat ttcggctttg
     1561 ccattcctac cggctcgacc atcgacggta tccaggccaa tgtcacgcat atggcctccg
     1621 ggcttagcct gacgattgga atactgacgg tcaccggttc ggttacagac aatattgtac
     1681 agttggtcgg tccgggtgtc acgtccgtca acaaggccag cggcgcgagc tggccgacga
     1741 gcaataccgt agcgtcctat ggctcgtcgt cggatgggtg ggggacgacc gtgtggaccg
     1801 ccgcgatggt caattccccc ggtttcgggg tggccgtatc ggcgaacatg aacggcaaca
     1861 cgctgctggg ggtggacctc atccccggag ctgctatcga tctggttacg atcaacatca
     1921 cctacagcct ccccgtcacc ctgcccgtgc aactggatca atgggcggtg gtccgccagg
     1981 gggatgtcga tgtccttcgg tggcaggcag gggcggatgc gatacccgcc tcgactcagg
     2041 cctccggcgc gccggcggag tttattgtcg agcgtagcgc gaactcatcg gactggagtg
     2101 atctggcgac gattgccgca tcctccggcc tgcggttata tacttacacc gatgaagaac
     2161 ccttcgccaa tgggcccacg tattaccggt tgcggttgca cagtgagggc cgtccggaca
     2221 gctggtcgac gatccaggtc cttgcggcaa agcaggtcca gcaccctgtg atcaacgtat
     2281 atcctaatcc ctttcataat acggtcaacg tctccgcggc ggatcccttc acccgtctca
     2341 ccctaaagga cagacagggt gcgacccttt gggtgaagga attctccggc ggggccaaca
     2401 aagcgcagct gccggccgcc ggcctgcccc aggggctcta cttcctgacc atcgacggag
     2461 caacttataa aatgatgaag gagtgatcgg gcgatccgcg gtccgcgatc ggggctgatc
     2521 gatcatcgca aacttacctt ggtagcccgc ttgcgggctc ctaccatggc ctctgggatc
     2581 aagcgatccg gcgttgtcta gcgccggata ttttttttgc atggctttcc tccgggctgc
     2641 cgctccgctc cctaacgcgg cgccttcagc ccccaccact cgccgccccg caaatttctc
     2701 tcttattcat acgacgcctg cggcgtctgc cggggaga
//
LOCUS       DATGFG010000133         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_320219, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000133 DATGFG010000000
VERSION     DATGFG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320219"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VKQ52_02430"
     CDS             <1..503
                     /locus_tag="VKQ52_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HLZ86063.1"
                     /translation="FLLNSPWLIAMKLKIISNQAAKERILTFFFGKQSLSRFEQYSEA
                     FSREALPELIRPKALTEIGRLREAGATIVIVSASPENWIKQWAARTGADLIATRLGVY
                     TDDKGEPRLTGRIDGANCHGEEKVNRIRQRFALPDYMEIYTYGDTSGDKPMLQLGTAS
                     FFRPFR"
     gene            536..1723
                     /locus_tag="VKQ52_02435"
     CDS             536..1723
                     /locus_tag="VKQ52_02435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ86064.1"
                     /translation="MDFVIVGLQAWYFELGSNCKNIALELSKHHRVLYINMPLDRRTI
                     WQKKDDPHIARHLHLIRKGHTEVVQLGPNLWNYSPTRVIESLNWLPSTFLFERFNRIN
                     NRRLAADIRKATAILGFKEYILFNDNDIFRSFHLKELLKPKLYIYYSRDNLLAVPYWR
                     KHGRTLEPLHIAKADIGVANSIYLADYLRQYNPNSYYIGQGCSLSVFNPNTTYPVPED
                     LKNIPHPIIGYVGAIVSLRIDIRIILEIARSRPDWHIVMVGPEDEFFAGSELHSLPNV
                     HFLGRKELPTLPAYIAHCDVCINPQLVNGMTIGNYPLKIDEYLAMGKPVVATRTATMK
                     LFEDHTYLADSPGDYVPLVEKALAEDNAGLQAKRIGFANEHTWENCLKEFYHAISKHP
                     LHE"
     gene            1716..>2771
                     /locus_tag="VKQ52_02440"
     CDS             1716..>2771
                     /locus_tag="VKQ52_02440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLZ86065.1"
                     /translation="MNNRRDDRILSLILVFGFLLRVIICFFTQLPNLHRDSYEYYGQA
                     DTLLAGGYTNFFPNGYPLIMAIAKAIAGTNSITLLLWVNITFSTLSIWFIYDIGKKVF
                     GNPSIALLAAAILAIFPSQLNYVRWLTTEVPTAFFLLGAYFFYYRRRYFRSGIFFALA
                     VFIRTNIAPIPIIILAVRAIRDKKLPWRLLAGVALPLMMVGSYCYWKTGEFSIAGNNQ
                     INILYAVTAKGDYLNLTLNREHPEINTTGKAVKMYIDHMKNDPLEFISQKGANFWELW
                     GFYPSSARGTRGMGSRILIGAGNSFLLLFGLAGWWIHRHSLQSNLLILPFVTVTFIHT
                     ILFALTRYTYPVEPFMIL"
BASE COUNT          666 a          896 c          601 g          608 t
ORIGIN      
        1 gattcctgct caatagcccc tggctcatag ccatgaagct aaagatcatt tccaaccagg
       61 cagccaaaga gcggatcctg acgttctttt tcggaaaaca atccttatcc cgcttcgaac
      121 agtatagcga agccttctcc cgggaagccc tgccggaact catccgccca aaagcgctga
      181 cggagatcgg tcggctccgg gaagcaggcg ccaccatcgt catcgtttcc gcctccccgg
      241 aaaactggat aaaacaatgg gccgcccgga caggcgccga tctcatcgca acccggctcg
      301 gcgtctacac cgacgacaaa ggagaaccca gactgaccgg ccgcatcgat ggcgccaact
      361 gtcatggaga agaaaaggtc aacaggatcc gccaacgatt tgctctgcct gactacatgg
      421 aaatatacac ctatggggat accagtggtg ataaaccaat gctgcaattg ggaacggctt
      481 cctttttcag accctttcgc tgaaaaaccg cccatcgcag ccaaaaaact aaactatgga
      541 ttttgtaatc gtaggtcttc aggcctggta ttttgaatta ggcagcaact gcaagaatat
      601 tgccctggaa ctatccaaac accaccgggt cttgtacatc aatatgccgc tcgaccggcg
      661 gaccatctgg caaaaaaaag acgaccccca tatcgccaga cacctccatc tgatccggaa
      721 aggtcacacg gaagtcgtcc aactcggccc caatctctgg aactactccc ccacccgcgt
      781 catcgaatcc cttaactggc taccctctac cttcctgttc gaacgcttca accggatcaa
      841 caatcgccgc ctggccgccg acatccggaa agcaacggct atcctcggct tcaaagagta
      901 tatcctcttc aacgacaacg atatcttccg ctccttccac ctgaaggaac tgctgaaacc
      961 aaaactgtat atctattata gccgggataa cctgctcgcg gtgccttact ggcgcaaaca
     1021 cggcagaacc ctcgaacccc tccacatcgc aaaagccgat atcggcgtag ccaattccat
     1081 ctatctggcc gactatctcc ggcaatataa ccccaacagc tattatatcg gccagggttg
     1141 ctccctgtcc gtcttcaacc caaacacgac ctaccccgtt ccggaggatc tgaaaaacat
     1201 tccccatccc atcatcggct acgtaggcgc gatcgtcagc ctgcgcatcg atatccggat
     1261 catcctcgaa atagcccgct cgagacccga ctggcatatt gtaatggtag gtccggaaga
     1321 cgagttcttc gccggcagcg aacttcattc ccttccgaat gttcacttcc tgggcagaaa
     1381 agagcttccc acccttccgg cctatattgc ccattgcgat gtctgtataa atccgcaact
     1441 cgtcaatggc atgaccattg gcaactatcc cctgaagatc gatgagtacc tggccatggg
     1501 taagcccgtc gtcgccaccc gcaccgccac catgaaatta ttcgaagacc atacctatct
     1561 cgcagacagc cccggcgatt atgttccgct cgttgaaaag gccctcgccg aagacaatgc
     1621 cggtcttcag gcaaaaagga tcggcttcgc aaacgaacat acctgggaga attgcctgaa
     1681 agaattctat cacgccatct cgaaacatcc tctccatgaa taaccggagg gatgatagga
     1741 tcctctccct catcctcgta ttcggtttcc tcctgcgggt gatcatctgc ttctttaccc
     1801 aactgcccaa tctgcaccgg gacagctatg aatattacgg ccaggccgat accctgctgg
     1861 ccggcggcta tacgaacttc tttcccaacg gctatcccct gatcatggcc atcgcgaagg
     1921 cgatcgcagg tacaaacagc attaccctcc tgctatgggt caacatcacc ttctccacgc
     1981 tgtccatctg gtttatctac gacatcggca aaaaggtatt cgggaacccg tccatcgccc
     2041 tgttggccgc cgctatcctc gcgatcttcc cctcccagct aaactatgtc cgatggctca
     2101 cgaccgaggt tcccaccgca ttttttctcc ttggcgccta tttcttctac taccggagac
     2161 gatatttccg gagcggcatc ttcttcgccc ttgccgtctt catccggaca aacatcgccc
     2221 cgattcccat catcatcctg gccgttcgcg ccatccggga caaaaagctc ccctggcgcc
     2281 tgctggccgg agtcgcccta cccctgatga tggtcggttc ttattgttac tggaagacgg
     2341 gagagttttc catcgccggt aataaccaga tcaatatcct ctatgccgta accgcaaagg
     2401 gcgactacct caacctcacc ctcaaccgcg aacatcccga gatcaatacc accggcaaag
     2461 cggtgaaaat gtacatcgat catatgaaga acgatcccct ggaattcatc agtcagaaag
     2521 gggcaaattt ctgggagctc tggggattct atccctcctc cgccaggggc acccgcggga
     2581 tggggtcacg catcctgatc ggcgcgggta attctttcct gctgctgttc ggcctcgccg
     2641 gatggtggat ccacagacat tcgctgcagt ccaacctgct gatcctgccc tttgtcacgg
     2701 tcaccttcat ccacacgatc ctattcgccc tgacacggta cacctacccc gtggagccat
     2761 tcatgatcct g
//
LOCUS       DATGFG010000134         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_666184, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000134 DATGFG010000000
VERSION     DATGFG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666184"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..472)
                     /gene="yidD"
                     /locus_tag="VKQ52_02445"
     CDS             complement(233..472)
                     /gene="yidD"
                     /locus_tag="VKQ52_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HLZ86066.1"
                     /translation="MRVVLMVLRILSLPLIALIKIYQWVISPAIGPRCRFTPSCSHYA
                     VEALKKHGLFKGGWLSIRRISRCHPWGGHGIDPVP"
     gene            complement(469..957)
                     /locus_tag="VKQ52_02450"
     CDS             complement(469..957)
                     /locus_tag="VKQ52_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HLZ86067.1"
                     /translation="MAKRATWKKAEKLKSRKHIERVFREGKSFSLFPYKVVFLLGPVR
                     AAAGEERMVAGEERRVAGEERMVGGEDRTVPQRAQFSALQAGFGASSRNFRKAVDRNR
                     IKRLGREAYRLQKAPLLDQLTRQGRSMAIFFIYIGKELPDYPTVTQKIGVALQKLIRE
                     TA"
     gene            complement(1016..1171)
                     /gene="rpmH"
                     /locus_tag="VKQ52_02455"
     CDS             complement(1016..1171)
                     /gene="rpmH"
                     /locus_tag="VKQ52_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HLZ86068.1"
                     /translation="MKRTFQPHRRRRKTVHGFRKRMQTANGRKVLASRRAKGRHKLTV
                     SDESKLK"
     gene            complement(1339..2238)
                     /locus_tag="VKQ52_02460"
     CDS             complement(1339..2238)
                     /locus_tag="VKQ52_02460"
                     /inference="COORDINATES: protein motif:HMM:NF039704.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HLZ86069.1"
                     /translation="MRKAIFLSIALLAGLASCKKETSIENGNTLSGNFTARIDGVQWS
                     ASGTKEGASILGGTINITGISADNKEISITIADSVAGVYTLNQTSASYGAYADIDSSD
                     LYAFATNQGSDTAQAGGSVTITEIDPVGKTISGTFAFKVYRDIDSRQKTITSGIFTKI
                     PYVTSLPATASSDTLRASIDGKAWVAVNIEATSSAGQLTIVGASSDASQTIGLLMPAT
                     AKAGTYPLDGSNPSYLGVYTVLANASSSGFASTKGTLTITQNNTSTSRISGSFQFTAA
                     DPTGASTATHVISSGIFSVYYGQ"
     gene            complement(2246..3067)
                     /gene="murI"
                     /locus_tag="VKQ52_02465"
     CDS             complement(2246..3067)
                     /gene="murI"
                     /locus_tag="VKQ52_02465"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HLZ86070.1"
                     /translation="MDNPIGVFDSGYGGLTVLKELVGRLPQYDYIYLGDNARAPYGNR
                     SFETVYQYTLQSVQWLFDQGCPLVILACNTASAKALRTIQQLDLPRMAPGNRVLGVIR
                     PTTEVVGQYSRSGKVGVLATRGTVLSESYPIEIAKFFPEVEVFQQPCPLWVPLIENNE
                     HDKPGADYFVKTYIDQLLARCAEIDTVVLACTHYPLLKDKIAAQLPKGSTLISQGEIV
                     AGSLAGYLRRHTEIESRCSKGGRVRFYTTDSTEDFDNHASIFYGHGLRSTHVLLT"
     gene            complement(3113..>3456)
                     /locus_tag="VKQ52_02470"
     CDS             complement(3113..>3456)
                     /locus_tag="VKQ52_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HLZ86071.1"
                     /translation="AQNFEDMKREYMEKDSLLNSKDTIKYTKAQLELKKREISDMLIK
                     LQGWQQQAQQLYQQKQQDLIQPIQKKAVDAVQTVAKENGYTYVLNKEILLTSPPADDL
                     LPLVKKKLGIK"
BASE COUNT          739 a         1022 c          910 g          785 t
ORIGIN      
        1 caaatagccc tgcacataca gccccgaacg aacagcccgg cacgtacggc cggaacagca
       61 cgaataaccc cgcacgaaca ccctcgctcg tacaacccgg cacgtgcagt cccgcgcgaa
      121 taaccctcca catacagccc ggcccataca gcaccgcacg aacatccccg cacgccatag
      181 cgccgccgcg gacccatcct tgcctacagc gccggctccc tatttgccgg tctcaaggca
      241 cagggtcgat cccatgccca ccccagggat gacatcggga gatccgccgg atactaagcc
      301 agccgccttt gaacaggcca tgttttttca acgcctcgac agcatagtgg gagcaactgg
      361 gtgtgaaccg gcaacggggc ccaatcgccg gcgagataac ccattgatag atcttgatca
      421 gggcgatcag gggaaggctt aagattcgca ataccatcaa taccaccctc atgcggtttc
      481 ccttattaat ttctgcaaag ctacgccgat cttctgggtt accgtcgggt agtccggcaa
      541 ctccttcccg atataaataa agaaaatggc catcgagcgg ccttgtcggg tcaattgatc
      601 cagcaggggg gccttttgca gacggtaggc ctcccggccc agccgcttga tccggtttcg
      661 gtcgacggcc tttctaaaat tccggctgct ggccccaaaa ccagcctgca aagcggaaaa
      721 ctgcgcccgc tgtgggaccg tccgatcttc ccccccgacc atcctttctt cccccgccac
      781 cctcctttct tcccccgcga ccattcgctc ttcccccgcc gcagcccgaa cgggacccag
      841 tagaaaaacc accttatagg ggaaaagcga aaaactcttc ccttcccgga acacccgctc
      901 gatgtgcttc cggcttttca atttctccgc cttcttccag gtagccctct ttgccataac
      961 gaaaaatagc ttcggacgat ccgaagctat tggcttataa tagattgtat aaattctact
     1021 tcagtttcga ttcgtcagaa acggtcagtt tgtgacgacc ctttgcacgg cgactggcca
     1081 gtactttacg tccgttggcg gtttgcatcc gcttgcggaa gccatgaacc gtcttgcggc
     1141 gacgtctgtg tggttgaaat gtacgcttca tttaaaaaat ttttacgagt tcgatatcta
     1201 atgccggtcg cttccgcggc cgctttttct tataccacgc ttcggggcac catccccgcc
     1261 gccggtgaaa gggagtgcga aattagggaa aattcgggaa aggtggaaaa aattggggaa
     1321 aaaccacgcc cggaacacct attgtccata atagaccgaa aagatcccac tactgatcac
     1381 atgcgtcgcc gtactggcgc ccgtggggtc cgccgccgta aactggaaac tgccacttat
     1441 ccggctggtg ctcgtattgt tctgcgtgat cgtaagcgtt ccctttgtcg aggcaaaccc
     1501 gctggacgag gcattggcga ggaccgtata gacacccagg tacgaggggt tggagccatc
     1561 gaggggatag gttcccgcct tcgccgtggc cggcatcagc aggccaatgg tctgactggc
     1621 atcggaactg gcgccgacaa tggtcagctg acccgcggag gacgtagcct cgatattgac
     1681 agccacccag gccttcccat cgatgctggc ccgcagggta tccgaggaag cggtcgccgg
     1741 caacgaagtg acatagggaa ttttggtaaa gatcccactg gtgatggtct tttgcctgga
     1801 gtcgatgtcc cggtacacct tgaaggcaaa ggttccggat atcgtcttgc cgacgggatc
     1861 gatctcggtg atggtcacac tcccgcccgc ctgggccgta tcgctgccct ggttggtggc
     1921 aaaggcataa agatcggagg agtcgatgtc ggcataggcc ccataggaag ccgaggtttg
     1981 gttgagggta tacactccgg cgacagaatc ggcgatggtg atgctgatct ccttattgtc
     2041 cgcgctgatc ccggtgatgt ttatcgttcc cccgaggatg ctcgcccctt ccttcgttcc
     2101 cgaggccgac cattggacgc cgtcgatcct ggccgtgaaa ttgccggaca gggtattgcc
     2161 gttctcgata ctagtctcct tcttacagga ggcaaggccg gcgagaagcg cgatcgaaag
     2221 gaagatggct tttctcatat ctgaatcagg ttaatagtac atgggtcgat cgcagtccat
     2281 gcccgtaaaa gatactcgca tggttatcaa aatcttcggt cgaatccgtc gtataaaagc
     2341 gtacccgtcc ccccttgctg caccggcttt cgatctccgt atgacgtcgc aaatagcccg
     2401 ccaaactgcc cgctacgatc tctccctgcg aaataagggt gctgcccttg ggtaattggg
     2461 ccgcgatctt gtcctttagc aacggataat gtgtacaggc caggacaacg gtgtctattt
     2521 cggcgcaacg cgcaaggagc tgatcgatgt aggttttgac aaaatagtcc gccccgggtt
     2581 tatcgtgctc gttattctct attaacggaa cccatagggg gcagggttgc tggaagacct
     2641 ccacttccgg gaaaaatttt gcgatctcga tcgggtagga ctccgaaagg acggtacccc
     2701 gggtcgccag caccccgacc ttacccgacc ggctatattg accaaccacc tccgtggtgg
     2761 gccggatgac ccccaggacc cggttcccgg gcgccatccg cgggagatcc agctgctgga
     2821 tggtccgcaa cgccttggcc gaagcggtat tacaggccag gatcaccagg gggcagccct
     2881 ggtcgaaaag ccattggacg ctttgcaggg tatactggta taccgtctcg aacgaccggt
     2941 tgccgtaggg cgcccgcgcg ttgtcgccaa gatagatata atcgtactgc ggcagccggc
     3001 caaccagctc cttcagaacc gtaagcccgc catagcccga gtcaaatacg ccgatgggat
     3061 tatccataca actagaacgc cggggtgggc cccggcgttt gtttgaaagt ttttacttga
     3121 tacccagttt cttcttcacc agtggaagca gatcgtctgc aggcggcgag gtcagcagga
     3181 tctccttgtt cagtacatac gtatacccgt tctcttttgc aacggtctgt acggcatcga
     3241 cagccttctt ttggatgggc tggatgagat cctgttgttt ctgctggtac agttgctgcg
     3301 cctgttgctg ccagccctgc aatttgataa gcatgtcgct gatctctctt ttcttcagct
     3361 ccagctgagc cttggtgtat ttgatcgtat cttttgaatt taaaaggctg tccttctcca
     3421 tgtactcccg cttcatgtct tcgaaattct gagcca
//
LOCUS       DATGFG010000135         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_431873, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000135 DATGFG010000000
VERSION     DATGFG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431873"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="VKQ52_02475"
     CDS             complement(<1..1771)
                     /locus_tag="VKQ52_02475"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HLZ86072.1"
                     /translation="MRIIPFLLSLILTTGLIVCLNRQWGGLKKTPPFGQFLSPQHGFW
                     QNAEPVNESYDGNVSLPGLKGKGDVYFDENLIPHVFAASQTDACYIQGYLHARFRLWQ
                     MEFQTFAAAGRVSEFLGPGEDNAYIKYDRSMRRLGMVTAAKAALGEMEKDSVVKADCD
                     AYTAGVNAYISQLKASELPLEYKLLDYSPEPWSNLKTSLFVKYMAYDLAGHDNDFEMT
                     NAKSFFSKEDFAKLYPEIQDSADPIIPKRTRFLPPAIAPKAPPTVDSLYLNHPMTVTA
                     AESKPDRDNGSNNWVVSGKKTSSGMPILCNDPHLSTNLPAIWYQMQIHTPTYNVYGVS
                     FPGAPYVIIGFNDRCAWGITNAERDVRDYYAIKFKDETRKEYWFNGKWEPAEQRVDTI
                     LVKGGVPFYDTVATTVFGPVMYDPTFPGFSDEGTTRSYAVHWKPAEPSDEIRTFSKFQ
                     SMHNYSEYKEAILTFKSPGQNFAFASKDSVIAIWQQGDFPAKWKEQGRFVMPGEDSTY
                     LWQGSIPQQENPTMLLDNEDNGRGFVSSANQLPVDTTYPYYLGIDFPPYRGLIINRRL
                     SSMNFITPADMMSLQTSNYDVFAE"
     gene            complement(2006..>2649)
                     /locus_tag="VKQ52_02480"
     CDS             complement(2006..>2649)
                     /locus_tag="VKQ52_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ86073.1"
                     /translation="RTVFVVTESVFSMDGDRAPLREMVALCRRYGAHLIVDEAHATGV
                     VGVVGEGLVQQLGLQEECFARIHTFGKAVGCHGAVVLGSVVLRDYLVNFSRSFIYTTA
                     LPPASVRAIAVAYALLPELGAERAQLQRLIGRWQAAPVGYRRLDSQTPIQVVIVPGND
                     AVRALAGRLQDAGLDVRPILYPTVPKGGERLRIVLHSFNTAEEVDRLTGLLQQ"
BASE COUNT          595 a          836 c          660 g          558 t
ORIGIN      
        1 tctccgcaaa gacatcgtaa ttgctcgtct gcagcgacat catgtcggca ggcgtgataa
       61 agttcatgct gctgagccgc cggttgatga tcagtccgcg atagggcgga aaatcgatcc
      121 cgagataata gggatacgtc gtgtccaccg gtaactggtt cgcactgctg acaaaacccc
      181 ggccattatc ctcgttatcc agcagcatcg tcgggttctc ctgctgcgga atgctcccct
      241 gccacaaata cgtactatcc tcacccggca taacaaaacg cccctgctcc ttccatttgg
      301 ccgggaagtc cccctgctgc cagatggcga tcacactgtc cttggaggca aatgcaaaat
      361 tctgccccgg gctcttaaag gtcaggatgg cctccttata ctccgagtaa ttgtgcatgg
      421 actggaattt gctaaaggtc ctgatctcat ccgatggctc cgccggcttc cagtgaacgg
      481 cataactccg cgtcgtaccc tcatcggaaa agccgggaaa ggtggggtcg tacataacgg
      541 gaccgaatac agtcgtcgcc accgtgtcat agaacgggac tccccccttc accaggatcg
      601 tatccacccg ctgctcagcc ggctcccatt tcccgttgaa ccagtattcc ttccgggtct
      661 catccttgaa tttgatggca taatagtccc gcacatcccg ctctgcattg gtgatccccc
      721 aggcacaccg gtcattgaac ccgatgatga catagggggc ccccggaaag ctgaccccat
      781 atacattgta agtgggcgta tggatctgca tctggtacca gatggccggc agattggtgc
      841 tgagatgggg gtcgttgcaa aggatcggca tcccgctgct cgtcttcttc ccgctgacga
      901 cccagttatt gctgccatta tcccggtccg gcttcgactc cgccgccgtg accgtcatcg
      961 ggtgattgag atacaggctg tccaccgtgg gcggcgcctt gggcgcaatg gcaggcggca
     1021 ggaaccgcgt cctcttcggg atgattggat ccgcactgtc ctggatctcg gggtatagct
     1081 tggcaaaatc ctccttgctg aagaagctct tcgcattggt catttcgaaa tcgttgtcat
     1141 gaccggcaag atcataggcc atatacttga caaaaagcga ggtcttgagg ttcgaccagg
     1201 gctcggggga atagtcgagc agtttgtact cgagcggaag ctcgctggcc ttcaactggc
     1261 tgatataggc attgaccccg gcggtataag catcacaatc cgccttgacg acgctgtcct
     1321 tctccatctc gccaagcgcg gccttcgcgg ccgtcaccat ccccagccgg cgcatactcc
     1381 ggtcgtattt tatgtaggca ttatcctccc ccgggccaag gaactcactc acccgccccg
     1441 ccgcggcaaa ggtctggaac tccatctgcc agagcctgaa tctggcgtgc agatacccct
     1501 ggatataaca ggcatccgtc tggctggcgg caaaaacatg tgggatcagg ttctcgtcga
     1561 aatagacgtc cccctttccc tttagcccgg gcaacgatac attcccgtca taactttcat
     1621 tcaccggctc ggcattctgc cagaacccgt gctgggggct aaggaactgc ccaaaaggag
     1681 gcgtcttctt cagcccgccc cattgccggt ttaggcaaac gatcagtccg gtagtcagga
     1741 taagggaaag gagaaagggg ataattctca tgccgctggt attaactggt attaatcggc
     1801 taaaaatagg gtatttttcc aacttccatt gactgccgga tgaaccatcg cttcattggc
     1861 cgaaggccat tcaaaattta ccacaaaatg gtattgccag agtccatact tcatctcatt
     1921 tttgcattgg taaaaacaac gtactcttta agttagttca ggtggttcag gtagatgaat
     1981 aggccggtct cagagatgag ccggcttatt gttgtaatag acccgtaagc cggtccacct
     2041 cctccgcggt attgaaggaa tgcagcacga tccgcaaccg ctcacccccc ttcggaaccg
     2101 tagggtacag tatcggccgc acatccaaac ccgcgtcctg caaccgcccc gccaacgccc
     2161 ggacggcgtc attccccggc acgatcacca cctggatagg ggtctgactg tccagccgcc
     2221 ggtagccgac aggagccgcc tgccagcgcc cgataagccg ttgcaactgc gcccgctcgg
     2281 cccccaactc cggcaacaac gcataggcga cggcgatagc ccgcacactc gcgggtggca
     2341 acgccgtggt atagatgaac gatcgcgaaa aattcaccag gtaatcccgc aacaccacac
     2401 tgccgagcac cacggcccca tgacaaccca ccgccttccc aaacgtatgg atgcgcgcaa
     2461 aacattcctc ctgtaatccc aactgctgca caagcccctc ccccacaaca ccgacaaccc
     2521 ccgtcgcatg cgcttcgtcg acgatcaaat gcgccccata ccgccggcaa agcgccacca
     2581 tctcccgcaa cggcgcacga tccccatcca tcgagaacac cgattccgtc accacaaaaa
     2641 cagtccgcc
//
LOCUS       DATGFG010000136         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_319993, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000136 DATGFG010000000
VERSION     DATGFG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319993"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VKQ52_02485"
     CDS             complement(<1..1000)
                     /locus_tag="VKQ52_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLZ86074.1"
                     /translation="MDINCTEKELFVFDKIARAAATLGAPAYLIGGFVRDKLIGRPTK
                     DADIVCVGDGIALAHKVAESLRPKPSVSYFKNFGTAQIKYEDWEIEFVGARKESYRSE
                     SRNPEVEPGTLEDDQLRRDFTINAMAISLNAADYGKLIDPFEGGKDLDTKLIRTPLDP
                     LKTFSDDPLRMLRAIRFATQLNFTIAEPTLRAIGEDAPRIKIVSQERITDELNKIILS
                     PIPSIGFDLLYKTGLLKIIFPQMVDLAGAEYIDGMGHKDNFYHTLQVLDNISRNTTDL
                     WLRWSAILHDIGKPATKKFEKEHGWTFHGHEVVGGRMVPKIFTRLKLPLNEKMRFVR"
     gene            complement(1047..2141)
                     /locus_tag="VKQ52_02490"
     CDS             complement(1047..2141)
                     /locus_tag="VKQ52_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLZ86075.1"
                     /translation="MQKFLVIQTAFIGDVVLATALIEKLHAFYPDTRIDFLVRKGNEG
                     LLTGHPYLNEVLIWDKKQNKLKNLFHLTRHIRKQHYDKLINVQRFAATGLLTAFSGAK
                     ETIGFDKNPLSRFFTHRIPHIVSAPTRATAPTSATAHTPGTPPTATTHTSAATLHEID
                     RNQTLIVAFTDAKPAKPRLYPTAADEERVKPYKAKPYITISPASVWFTKQYPKERWAS
                     FIRQLPADLIVYLLGAPTDRELCQSIFAENPSPLVVDLSGQLTFLQSTALLRDAAMNY
                     VNDSAPMHFASAVNAPVTAVYCSTIPAFGFGPLSDKSYIVEIAEPLDCRPCGLHGYKA
                     CPRGHFNCAYKIQDQQLLQTLITSHPKGAR"
     gene            complement(2151..2852)
                     /locus_tag="VKQ52_02495"
     CDS             complement(2151..2852)
                     /locus_tag="VKQ52_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86076.1"
                     /translation="MDNPWHRRIGGLALLLLAAFASYGQATKKSVFEHPDNEYGNSIY
                     KIFGISRAIPIKQGYIVTKMADTLWGYIDMNKFDISEKLRQVAILPFGKQNRSDIRIV
                     QLDSIDYISTKPQQLADSGRQEYMPVDGAMWRLVGRSACVRICGRYGILNSDNSYDLI
                     VNEAMVMFSCNHRIEIPVAGYLSLHSDLYFFAKFVRQRYGTNIAPRDLKHKDPIQVIL
                     AKENERLRMHLGKTS"
     gene            complement(2991..>3392)
                     /locus_tag="VKQ52_02500"
     CDS             complement(2991..>3392)
                     /locus_tag="VKQ52_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HLZ86077.1"
                     /translation="VLMADKRDILKYTSQPDLRIFDFLDTVQKPTTIIYEGAIGLAPN
                     LVNADGTIAIRLVDDPFCRHLIKRFRKPLVSTSANISGEPAPAFYREIDPRIRTGVDY
                     IVEHRRDDETPREPSAIIQWNPNGPPTVIRP"
BASE COUNT          626 a          732 c         1185 g          849 t
ORIGIN      
        1 tgcggacgaa ccgcattttt tcgttgagcg ggagcttgag cctggtgaag atctttggca
       61 ccatacgacc gccgacgacc tcatgaccgt ggaatgtcca gccatgctct ttttcgaatt
      121 tctttgtcgc cggcttgccg atatcatgca ggatagccga ccagcgcagc cagagatcgg
      181 tggtgttccg gcttatgttg tcgaggacct gcagggtatg gtagaaatta tccttgtgcc
      241 ccatcccatc gatgtattcc gctcccgcca ggtcgaccat ctgggggaag atgatcttca
      301 ggaggcctgt tttataaagc aggtcgaagc caatggaggg tatggggcta aggatgatct
      361 tgttgagttc atcggtgatc cgctcctgtg aaacgatctt tatccggggt gcgtcttcgc
      421 cgatggcccg gagggtgggt tctgcgatgg taaagttcaa ttgcgtggcg aagcggatgg
      481 cccggagcat acgcagcggg tcgtcgctga aggtcttcag ggggtccagc ggggtgcgga
      541 tgagctttgt gtcgagatct ttccctcctt cgaaggggtc gatgagtttg ccatagtcgg
      601 cggcattgag gctgatggcc atggcgttga tggtaaagtc gcggcgaagc tggtcgtcct
      661 ccagggtgcc gggttcgacc tcgggattgc gggattcgga ccggtagctc tccttacggg
      721 cgccgacgaa ttctatttcc cagtcctcgt actttatctg ggcggtgccg aagttcttga
      781 aatacgatac ggagggtttt ggtcttaggg attcggctac cttatgcgca agggcgatgc
      841 cgtcgcccac acagacgatg tccgcgtctt ttgtagggcg gccgatgagt ttgtcgcgga
      901 cgaagccgcc gatcaggtag gccggggccc ccagggtagc ggcagcccgg gcgatcttat
      961 cgaagacgaa caattccttt tctgtacagt tgatgtccat agttgtgagc cggtcaggcg
     1021 ccggctttgc cggaaggttg ccggcgttag cgggcccctt tggggtgcga agttattaaa
     1081 gtttgcagaa gttgctggtc ctgaatttta taagcgcaat taaagtgtcc gcgggggcag
     1141 gccttatagc catggagccc gcaggggcgg cagtcgaggg gttcggcgat ctcgacgata
     1201 tagcttttat cgcttagggg gccgaatccg aaggcaggga tggtggaaca gtagaccgcg
     1261 gtgacggggg cgttgacggc ggaggcgaag tgcatcgggg cggagtcgtt gacatagttc
     1321 atcgcggcgt cgcgaaggag ggcggtggat tgaagaaagg tgagttggcc ggagaggtcg
     1381 accaccaggg gtgacgggtt ctccgcgaaa atgctttggc agagttcgcg gtccgtcggg
     1441 gcgccgagaa gatagacgat cagatccgcg gggagttgtc gtatgaagct cgcccatctt
     1501 tcctttgggt attgttttgt gaaccagacg gaggcgggtg agatggtgat atagggtttt
     1561 gctttgtagg gttttaccct ttcttcgtct gcagctgtag gatagagccg gggtttggcg
     1621 ggtttggcgt cggtgaaggc cacgatgagg gtctggttgc gatcgatctc atggagggtc
     1681 gcggcggatg tgtgggtggt ggcagttggt ggggtgccgg gtgtgtgggc ggtggcggat
     1741 gtgggtgcgg tggcgcgagt cggggcgctg acgatatgcg ggatgcggtg ggtgaagaag
     1801 cggctgaggg ggttcttgtc gaagccgatg gtctcttttg cgccggagaa ggcggtgagg
     1861 aggcccgtgg cggcgaagcg ctggacgttg atgagtttgt cgtagtgttg ttttcttatg
     1921 tggcgcgtga gatggaagag gttcttcagt ttgttttgtt ttttgtccca gatgaggacc
     1981 tcgttgaggt agggatggcc ggtgaggagg ccttcgttgc ctttgcgtac gaggaagtcg
     2041 atccgggtgt cggggtaaaa cgcgtgaagc ttttcgatga gggccgtggc gaggaccacg
     2101 tcgccgatga aggcggtttg tataaccagg aatttttgca cggggcaaat ttagctggtt
     2161 tttccgaggt gcatgcgaag ccgttcattt tctttagcga ggataacctg tatcgggtct
     2221 ttgtgtttca gatcgcgagg ggcgatgttt gttccatagc gctgacggac aaatttggcg
     2281 aagaagtata ggtctgagtg gagggagagg tatcctgcca cagggatctc tattcgatgg
     2341 ttgcaggaga acataaccat ggcttcgtta acaatcaggt cgtagctatt gtcactgttg
     2401 agaatgccgt accttccgca gatgcgtacg caggcgctcc tgcccacgag acgccacatg
     2461 gcgccatcaa ccggcatata ctcctggcgt cctgaatccg ccaattgctg gggtttggtg
     2521 ctgatataat cgatggaatc caattggacg atgcgtatat cgctccgatt ttgtttgccg
     2581 aagggcagga tggccacctg gcgcagtttt tcgctgatat cgaatttatt catgtcgatg
     2641 tatccccaca acgtgtccgc catcttcgta acgatataac cttgttttat aggaattgcc
     2701 cggcttatgc cgaatatctt gtaaatggaa ttgccatatt cgttgtcggg gtgctcaaat
     2761 accgacttct ttgtggcctg cccgtaagag gcaaaggcgg cgaggagcag taaggcgagg
     2821 ccgcctattc ttctatgcca ggggttatcc atgcgctgaa attacgcgag ggcggcaggg
     2881 atgtcaagca cttttgcgta gtttaatcgg ggtttaaccg gaaattaatc cctttttcat
     2941 gcgtgggccg gccgggtatg ccggtgcgga tgacgagggg atgcgggtga ttagggtcgg
     3001 atgaccgtgg gcgggccgtt ggggttccat tggataatag ccgagggttc gcggggtgtt
     3061 tcgtcgtcgc ggcgatgttc gacgatatag tcgacgccgg tgcggatgcg gggatcgatc
     3121 tcgcggtaga aggcgggggc gggttccccg gagatattcg cggaggtgga gacgaggggt
     3181 ttgcggaagc gttttatcag atgccggcag aaggggtcgt cgacgaggcg aatggcgatg
     3241 gtgccgtcgg cattgacgag gttgggcgcg aggccgatgg ccccttcata gataatagtc
     3301 gtgggttttt gcacggtgtc gaggaagtcg aagatgcgga gatcgggttg gcttgtatat
     3361 ttcaggatgt cgcgtttgtc ggccatcagc ac
//
LOCUS       DATGFG010000137         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1458475, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000137 DATGFG010000000
VERSION     DATGFG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458475"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3116
                     /locus_tag="VKQ52_02505"
     CDS             <1..3116
                     /locus_tag="VKQ52_02505"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HLZ86078.1"
                     /translation="FFIILFGDNPHTAARGVNFMSAIASGLSILFLFWTITHFARKLV
                     MGGHTQAGGAPSYNPDAEPDSRQTFTILAAGVVGALAYTFCDSFWYSAVEGEVYASSA
                     FFTALVFWAILKWEHNADKPGSDKWIIFIFYMMGLSIGVHLLNLLTIPAIVMVYYFKR
                     FRPTPWGTIIAFLIGCLITGVVQVVVIQYTIKGAGAFDIFFVNSFNTPFFTGFGFYFG
                     LLAALLIWGLLLKNETFTATRIRIWIAAFVLIIMIPVISNSTGGGRFLKLLIGLGAAW
                     VAGYFIKKDALPKFKIALWCFAFMLLGYSTYFTTLIRSNADPGVDMYNVDNPVSLVGY
                     LSRDQYGDWPILYGPDFQDRAPRVDAGPLYVKGKGKYEVAGKLYGSDWGNTPSSHIFP
                     RMWDGGNDRGQVDCYRFFGGVADGENPSMVDNIRYFFKYQNGVMFLRYFMWNFSGKQN
                     DLQGFGTNPRDGNFITGISFIDNLMYGDQGSMPDSIRKNNKSYNRMFLLPFILGLIGL
                     VYQASRNKGDFWVTGLLFFFTGFAIVVYLNQAGYQPRERDYAYAGACYAFAIWIGLGV
                     IWVKEFLEKAAFKGKADMANYAAAGLCFLAVPVLMGSQEWDDHDRSKKTLARDIGRDY
                     LESCPPNAILFSFGDNDTYPLWYAQEVEGIRPDVRVMNYSLLGTDWYINQLRYKVNES
                     GPTDVMFTPEQIQGNTRDAVVINALNGFDQNKYYDLYDVLKNVVGSDDSRYQTQSEEG
                     DVYNFLPTRKLSVPVDSSLVRTNGTVMPGDSIVHELHLDIGKNKNYLFKNELAVLAII
                     AANKWQRPICFNSTYELEDLGLSKYIRQDGLAGRLVPVESKNTNFGFYNNTVAYNNMM
                     TKFGYGNAADPGVYYDEENRRHLNTLRAAHAQLALSLIEAGQKDSARNLLEHFDKNVY
                     EPNFPYGMTSNRGNQHDRISATFLAACYQSGDLALAQKVAASLKKDLNQQMRYYASLG
                     ENMTNEQLAINAQMALQGKGGNLSERQGGSIAQDILSTYQMLLQLEDWDRQYGHAKPS
                     GVQQPAK"
BASE COUNT          718 a         1071 c          778 g          673 t
ORIGIN      
        1 gcttctttat tattctcttt ggtgacaacc cgcataccgc cgcccgcggc gtcaacttca
       61 tgagcgccat cgccagcggc ctttccatcc tcttcctgtt ctggaccatc acccactttg
      121 cacgcaaact cgtgatgggt ggccacacac aggccggcgg cgccccttcc tacaatcctg
      181 acgccgaacc cgacagccgt cagaccttta ccatactggc cgccggtgtc gtcggcgctc
      241 ttgcctatac cttttgcgac tccttctggt atagcgccgt cgaaggggag gtatatgcct
      301 cctccgcctt cttcaccgcc ctcgtcttct gggccatcct gaaatgggaa cacaacgcgg
      361 acaaacccgg ctccgataaa tggatcatct tcatcttcta tatgatgggc ctctccatcg
      421 gcgtccacct gctcaacctg ctgaccatcc ccgccatcgt gatggtctac tacttcaaac
      481 gtttccgccc caccccctgg ggcaccatca tcgccttcct catcggctgt ttgatcacgg
      541 gcgtcgtcca ggtcgtcgtc atccaataca cgatcaaggg cgccggcgcc ttcgatatct
      601 tctttgtcaa ctccttcaat acccccttct tcacgggctt cggattctat ttcggcctcc
      661 tggcggccct cctcatctgg gggctcctcc tgaagaatga aacgttcacg gccacccgca
      721 tccgtatctg gatcgccgcc ttcgtattga tcatcatgat cccggtgatc agcaattcca
      781 ccggcggcgg ccgtttccta aagctcctca tcgggctggg agccgcgtgg gtagccggct
      841 attttataaa aaaggacgcc ctgcccaaat tcaagatcgc gctctggtgc ttcgccttca
      901 tgctcctggg ctactccacc tatttcacga ccctcatccg cagtaacgcc gatcccggcg
      961 tggatatgta caacgtggac aaccccgtga gcctcgtcgg ctacctcagc cgcgaccagt
     1021 atggcgactg gcccattctc tacggccccg acttccagga ccgcgccccg cgcgtcgacg
     1081 ccggcccact gtatgtaaaa ggaaaaggca aatacgaagt ggccggaaag ctctatggca
     1141 gcgactgggg taacaccccc tcctcccata tcttcccacg catgtgggac ggtggtaacg
     1201 atcgcggcca ggtcgactgc taccgcttct tcggcggcgt tgccgacggc gagaacccgt
     1261 ccatggtgga caacatccgc tatttcttca aataccagaa cggcgtgatg ttcctccgct
     1321 acttcatgtg gaacttctcg gggaaacaga acgacctcca gggcttcggc accaaccccc
     1381 gcgacggcaa ctttatcacc ggtatttcct ttatcgataa cctgatgtat ggcgatcagg
     1441 gcagtatgcc cgactccatc cggaagaaca acaaatccta taaccggatg ttcctcctgc
     1501 cctttatcct cgggctgatc gggctggtct accaggcctc ccggaataag ggggacttct
     1561 gggtgacggg attgctcttc ttctttaccg gtttcgccat cgtcgtctat ctcaaccagg
     1621 ccggctacca gccacgcgaa cgggattacg cctacgcagg cgcctgctat gccttcgcca
     1681 tctggatcgg cctcggtgtc atctgggtaa aagagttcct ggaaaaagca gccttcaagg
     1741 gaaaagcgga catggccaac tacgccgccg cgggcctctg cttcctggcc gtacccgtgc
     1801 tgatgggcag ccaggaatgg gacgaccacg atcgcagcaa aaagaccctc gcccgggaca
     1861 tcggccgcga ctacctcgaa tcctgcccgc caaacgccat cctcttctcc tttggcgata
     1921 acgacaccta ccccctctgg tacgcccagg aagtggaagg catccgcccc gacgtccgcg
     1981 tcatgaacta tagcttattg ggcaccgact ggtatatcaa ccagttacgt tataaagtaa
     2041 acgaaagcgg gcccaccgac gtgatgttta cacccgaaca gatccagggg aacacccggg
     2101 acgccgtcgt catcaacgcg ctcaacggct tcgaccagaa caaatactac gatctctacg
     2161 atgtcctgaa gaacgtggtc ggcagcgatg actcgagata ccagacccag tccgaagagg
     2221 gcgatgtata caatttcctc cccacccgca aactctccgt tcccgtggac tccagcctgg
     2281 tccgcaccaa cggaaccgtg atgccggggg atagcatcgt ccacgaactc catctcgata
     2341 tcggcaagaa caagaactac ctgttcaaga atgagctggc cgtcctcgcc atcatcgccg
     2401 caaacaaatg gcaacgaccc atctgcttca actccaccta tgagctggaa gacctcggcc
     2461 tgtccaaata tatccgccag gacggactgg ccggccgcct cgtacccgtg gagagtaaga
     2521 acaccaactt cgggttctac aacaacacgg tggcgtacaa caacatgatg accaaattcg
     2581 gttatgggaa tgctgctgac cccggcgtct actacgacga ggagaaccgc cgccatctca
     2641 ataccctgcg cgcggcccac gcccagctgg ccctcagcct tatcgaggca ggccaaaagg
     2701 attccgcccg caatctcctg gaacatttcg acaagaacgt ctacgaaccc aatttcccct
     2761 atgggatgac gtccaaccgc ggcaaccagc acgaccgcat ttccgcgacc ttccttgcgg
     2821 cctgctatca gtccggcgat ctcgcgctcg cccaaaaggt ggccgcatcc ctgaaaaagg
     2881 acctcaacca gcagatgcgc tactacgctt cgctcggtga gaacatgacc aacgaacaat
     2941 tggccataaa cgcccaaatg gccctgcagg gcaaaggcgg gaacctctcc gagcgccagg
     3001 gagggtccat cgcacaggat atcctcagta cttaccagat gctgctgcaa ctggaggact
     3061 gggatcgaca atacggccac gccaaaccct caggagttca acagcctgct aaataagaca
     3121 acaaaaaccc gcttccgagc gggtttttta gtgcccggac ggacccaacc atttcattga
     3181 ataagttgtt tttaatctgt aaattgtagc acatgatagg cttccacttc aagaattatg
//
LOCUS       DATGFG010000138         9310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_657368, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000138 DATGFG010000000
VERSION     DATGFG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657368"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..2785
                     /locus_tag="VKQ52_02510"
     CDS             410..2785
                     /locus_tag="VKQ52_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86079.1"
                     /translation="MIKNYLKIAWRNLIKSKVFSFINIFGLTIGITVCGMIFLFIMNE
                     FSFDNFHKNGEDIYRVMRSFDANKPAAPWLSGPYEPALLNDFRGEIKQAVRVMPDNDL
                     VSYKNRPFNEKNVYIVDSNFFTFFSFPLLKGDPATALRDENSVVLTEATAKRIFGNED
                     PMGKVIDWNQDRKLTVTGIARDVPGNSHLNFDIVIPMARLYNQPFFKVWINNNHFLYV
                     QLDKQANRASIERRFPAFMDKYMGPTMKQTGYKFALSMIPLKDVYFGGDSAFDNVRHG
                     SKKVVYVFLSIAVLILLIACINFMNLSTIRAAERSKEVGLRKVMGALRKTLAGQFIGE
                     SLLLAVISCVLSIGLLQLLMPLYSDLLGYPLSVPYASGYIYAFLIGTIVVVGLLAGSY
                     PALILSGFSPIEALKGKLKLGKGGSLFRQTLVVVQFSISVFLITGTVIITRQMSFIKN
                     QDLGYNQEQSLIIPLDNRDIGNNMDRFKKDLQANSNIASVSTMSGEPGGFFDVHTFDV
                     EGQDNKVWKGRTEFADFEFVKTLGLKIIAGRDLSSQFPTDTVSAVLLNRMAAADLGWT
                     PQQAIGKWIKNTVRDSLRRRVVGVVEDFNYTSLKENIEPLVISPSMDRRVVLVRLKPG
                     NLQNNVALVKKAYAGVAPAYPFEYTFLDQKFDLLYRKDIRQQTILSIFAGLAIVIACL
                     GLFGLASFTAVKRTKEIGVRKVLGSSVQNIILLLSKDLLKPVLLATLIAVPVAYFVMH
                     GWLQNFAYRTGLQWWIFALAAGITVAIALATVSFRAVKAAMANPTKSLRSE"
     gene            3077..3319
                     /locus_tag="VKQ52_02515"
     CDS             3077..3319
                     /locus_tag="VKQ52_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86080.1"
                     /translation="MADIPATAANSPNLFLSGTKLSNAPITLGPDPLYGNMQYSITDC
                     RDAPGTWNIINNTGGTPAVNPLTDTDITEVLPYFNL"
     gene            complement(3304..3981)
                     /locus_tag="VKQ52_02520"
     CDS             complement(3304..3981)
                     /locus_tag="VKQ52_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86081.1"
                     /translation="MKKNTITILACFALSVVQGQMKTVLPAYVILKSLEFPINKYYND
                     EYYIKIEKQLSDESLQKIAKEVRLKRAIDSHVGATYHFVQDNKVVCILKDNFKDIKGY
                     RCFRNDTASFSLEKMLDSIRLKTYRSLPDSILGYWSSASNAFVGLMIIYKTGAGWTEQ
                     NYFANGVEGYSRKLEVKELDGKKYFCFKTDGDPGYYFINEYGDIEFYADSSGFKMAFY
                     NTYYRLK"
     gene            complement(4043..6403)
                     /locus_tag="VKQ52_02525"
     CDS             complement(4043..6403)
                     /locus_tag="VKQ52_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86082.1"
                     /translation="MFRNYFKTAWRNLVKNKFYSLINMAGLTAGLAIGILILLWVEDE
                     RSFDSFHKKAADIYRMELFGGTGASKQILSMTVAPMGPLAKKELPEVTEQVRMTSNNY
                     ATLYKYHDKSFADQRAMFTDPSFFTLFDFPLVEGNPELPFPDAHSVVITRTTARKFFG
                     DEPALGKVIGTDNNDNYTVSGVINDLPLNTSIQFDMLMPMTLMLQKWQESRIDLNHDF
                     NSYMYITFLQLKHGVSPAGLGARLNQVHLRNKPEDTDADYLLLPLNKMHLYNADGSDA
                     GAQTVTIFMIIALLILAIACINYVNLSTARAMLRSKEVSMRKIVGAAKIQLFLQFVVE
                     TALLFLFSAVLAVVLVRLAMPLFNQLAGKQLVFNIGSARMWTVILCTIGATLVASSIY
                     PALLLSSFNPLKALKSKLTISRGDAAFRKVLVVIQFASSVILIIGTLVIYRQLNYIRH
                     KDLGYDRSHVFSFSMRNMRPAYETAKGKLLGLTGVQGVTASTNTIIDLRGISGDNDWD
                     GKGADQTFIVHPIAVDKNFLSFFKMQLAEGEGFTGTNADSTHFLLNETAIKEIGLSHP
                     IGKRFRLGKTNGTIVGVVKDFHFGSMKQKIAPAIFYSLPSWWLNRMYVRTTGENAADA
                     IRAAAALFKQYNADYPFSYAFLDESFNQLYQSEEREGTLFFYFAAIAIMISCLGLFAL
                     AAYATHLRFREIGIRKVLGASVPGIITLLAKDFVRLVLLGILIAVPIAWYAMDRWLAA
                     FAFRTDLGWPVFAVAGGITLAVALMTISFQAVKAAAANPVISLKTE"
     gene            complement(6469..8235)
                     /locus_tag="VKQ52_02530"
     CDS             complement(6469..8235)
                     /locus_tag="VKQ52_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86083.1"
                     /translation="MKIVNITPHAMSGETHQDSNPNIAVNPRNPNQIVISVLTPDPNN
                     GPNAPLYVSIDGGDTWALRGIVPGNGPEGTLGITVKYGQSSNELYAGVLSGIDSSSTI
                     LRTTNPVAGTVMTVLNSQQVGGKQQLYVAATTVASGPDAGKDRLYVGMNDISGPGGQG
                     EETSGQTSIIRQTMDARAAVPVFSSISLDVRPKNLGALQDLIPRQNGPEVRPAVHRDG
                     TVYAVFYSWKSWADSGVDWSSVSSDVVIVRDDNWGQSANPYGSLIDGVDSLAGQRIAI
                     GVPLLINDVIAQERMGGALAIAVDPGNSSIVYVAWGEQPDDPDTGQTPVALKLHLVRS
                     TDRGQTWSNPDLLTIGNGKNPGLAVNDRGQIGFLYQQFTGSDADGPDSHQRWETHLAF
                     SADGVHWSDTLLARVPADMPIRQFFPYIGDYADIMAVGNTFYGVFCANNTPSLPNFPQ
                     GVHYQRNHNFATATLSDLHGVPVAASIDPFFFKTEFIPPALQMPSLNSVGIILIILFG
                     IINDGGGVYIDGSGHIHIVGPGNPGPVWEYLVSLAEYRLATASNTKAGLEMQKLALQN
                     VIDLANAQIAQINNQFGQVRGE"
     gene            complement(8361..>9310)
                     /locus_tag="VKQ52_02535"
     CDS             complement(8361..>9310)
                     /locus_tag="VKQ52_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ86084.1"
                     /translation="YGTLPMPERANIRYWDLPTGSRIAFTLIPAKGVKKPYPIIYLQG
                     GPGGSVSEGLIRIMTPFSEDGFDVYVYDQIGSGWSGRLADIREYTAGRHKRDLEAIVQ
                     ATGAEEVILIGQSWGAILAALYAADNPGRVAKMILTGPGPLPPFHPELAKLPAPDSLH
                     LREPYYTNHQGNEEANNLRTRTMEMLATSFGKKLASDREADDFACYLGSKVNRSTVCD
                     TSLIEHTQPRGGAGFYVQIMTVFSCRSLPDPRPKLRNSPIPILVLKGQCDNQKWGFTN
                     EYLQLFPHHRLVVIPDAGHGIYGEQPERYLAAIKEFLKE"
BASE COUNT         2228 a         2554 c         2348 g         2180 t
ORIGIN      
        1 cagtatcccg aagaggacca gacatagttt acaataatcg acagccgcca gggcgaaagc
       61 tctttgcagg ctattgagta ataggttgcc ggcaacggta aaaaaggatc ccatgtccaa
      121 aaagcgccag gctataggcc aggatgggca tacgcagatc ataatccccg ggtgtttgta
      181 tcaccctcct cccaagaccc cagcggcctg caggggcccc ttacgatcgc aggaaaccgt
      241 ttttgttacg gcaaaaccgc acggctactg tatcgaaatc gaacaaaagg gtgcaaaacc
      301 acaaatcaat tagctgattg gtaaatgttt acagaagcgg catgaacttt agtgcttgga
      361 atatctctcg acaacgatac ccacaaccgg cccgtccata aaaaattcta tgataaaaaa
      421 ttatctgaaa atcgcctgga gaaacctgat aaagagcaag gtcttttcct ttatcaatat
      481 tttcggcctg acgatcggca tcaccgtttg cgggatgatc ttcctgttca tcatgaacga
      541 gtttagtttt gacaacttcc acaagaacgg ggaagatatc taccgagtca tgcggtcctt
      601 tgatgccaac aaacccgcag cgccctggct ttcagggccc tacgagcccg cattgctcaa
      661 tgacttccgc ggcgagatca aacaggccgt ccgcgtcatg ccggacaacg acctggtttc
      721 ttataagaac cgccccttca atgaaaagaa cgtctacatc gtggacagca actttttcac
      781 ctttttctct tttccgctgc tcaagggaga cccggccacc gcactcaggg acgagaacag
      841 cgttgtattg acggaggcca ctgcaaaaag gatctttggc aacgaagacc ccatgggaaa
      901 agtgatcgat tggaaccagg accggaagtt gacggtcacc ggcattgcca gggatgtacc
      961 gggcaactcc cacctgaatt tcgacatcgt gatcccgatg gcccgtttgt acaatcagcc
     1021 ctttttcaag gtctggatca acaacaacca ttttctatac gtgcaactgg ataagcaggc
     1081 caaccgggca agcatagaaa gacgttttcc ggcctttatg gataaataca tggggccgac
     1141 catgaagcag acaggctaca aattcgcact ctcgatgatc cccctgaaag acgtttattt
     1201 tgggggtgat tctgcctttg acaatgtaag acacggcagc aaaaaagtag tctatgtctt
     1261 tttgagcatc gctgtgttga tcctgctcat cgcctgcatc aattttatga acctgtcgac
     1321 catccgcgcc gccgaacgca gtaaggaagt aggcctgcgc aaggtgatgg gcgcgctccg
     1381 caaaacattg gccgggcaat tcatcggcga gtcgctgttg cttgccgtca tctcctgcgt
     1441 gctatcgata ggcctgctcc aattgctgat gccgctgtac agcgacctgc tcgggtaccc
     1501 gctcagcgta ccctatgcct cggggtacat ttacgctttt ctcatcggca ccatcgtggt
     1561 cgtgggcctg ctggccggta gctatcccgc cctcattctt tcggggttct cgcccatcga
     1621 ggcattaaaa ggtaagctaa agctggggaa aggcggctcc ctcttccggc agaccctggt
     1681 cgtcgtacag ttcagcatat cggtgttcct catcacagga acggtgatca tcaccaggca
     1741 gatgagtttc ataaagaacc aggacctcgg gtataaccag gaacaatccc tgatcatccc
     1801 gctggacaac cgtgacatcg gcaacaacat ggacaggttc aaaaaggacc tccaggccaa
     1861 cagcaacatc gcctccgtat ccacaatgtc cggcgaaccc ggcggcttct tcgacgttca
     1921 cacgtttgat gtcgaaggac aggataataa agtttggaaa ggaaggacgg aattcgccga
     1981 tttcgaattt gtcaagaccc tgggtctgaa gatcatcgcc ggccgggatc tctcctcgca
     2041 attcccgacg gataccgtat cggccgtgtt gctgaaccgg atggcggcgg cggacctcgg
     2101 ttggacgccg cagcaggcca tcgggaaatg gatcaagaat acggtgcggg acagcctgcg
     2161 ccgcagggtt gtgggcgtag tggaggattt caattatacc tcgctcaaag agaatatcga
     2221 gccgctggtg atctcgccaa gcatggacag aagggtagtc ctcgtgcggc taaagccagg
     2281 caacctgcaa aacaacgtgg cgctggtgaa aaaagcttat gccggtgtag cacccgctta
     2341 cccgtttgaa tacaccttct tagaccagaa attcgacctg ctctaccgga aagatatccg
     2401 ccagcagacc atcctcagca tcttcgcagg gctggcgatc gttatcgcct gcctggggct
     2461 ttttgggctg gcatcgttca ccgccgtaaa acggacaaaa gagatcggtg tgcggaaagt
     2521 gctgggctcg tcggtacaaa atattatcct gctgttgtcg aaggatctgc tgaaacccgt
     2581 cctgttggcc accctcattg ccgtccccgt cgcttatttc gttatgcatg gctggctgca
     2641 aaattttgca taccgcaccg ggctgcaatg gtggatcttt gcattggcgg cagggataac
     2701 tgtcgccatc gccctggcca cggtcagctt cagggcggtg aaagcggcga tggccaatcc
     2761 gaccaaaagc ctgcgaagcg agtagcgtcc gaatccattc atggctgtct gtttgcattg
     2821 atggctatct gtttaccttg aaagcacgga ttgtttttgt cttataatta acattatgtt
     2881 aaatacaaat cctggcaact accagtcccc atcccatggt atttttccgc cctatccttt
     2941 cggcgatgca taccctaata caggagaaga tcaacattgc aacgcaggat gaaaagaacc
     3001 agcagaattt gatccagaat gctcaggacg tacatttacc agtgggctca acataaaaat
     3061 atctgccgtt tccattttgg cggatatccc cgcaacggcc gccaattctc cgaatctctt
     3121 tttgtccggc acaaagctct ccaatgcgcc cattacgctg gggcccgatc cgctatacgg
     3181 gaacatgcaa tacagcataa cggattgcag ggatgcaccc ggcacatgga atattatcaa
     3241 taacaccggc ggcacaccgg cagtcaatcc ccttaccgac acggatatca ccgaagtatt
     3301 gccttatttt aacctgtaat aagtattata aaatgccatt ttaaaccctg atgaatcggc
     3361 ataaaattcg atatctccat attcattgat aaagtaatat ccgggatcgc catcggtctt
     3421 aaaacagaag tattttttcc cgtcaagctc ttttacctcc agttttcggg aatacccttc
     3481 taccccattg gcgaaatagt tttgttctgt ccaacctgcc cctgtcttat aaatgatcat
     3541 cagtcccacg aaagcgttgg acgcactgga ccaatagccc aagatgctgt cgggtaggct
     3601 tcgataggtt ttcaacctga tggagtcaag cattttttcc aggctgaatg atgccgtgtc
     3661 attcctgaaa caacggtatc cttttatgtc cttgaaattg tccttcagta tacagacaac
     3721 cttgttgtct tgtacaaaat gatatgttgc gccgacatgg ctgtcaatag cgcgctttaa
     3781 ccggacctcc tttgctatct tttgcaacga ttcgtcactt agttgtttct ctattttaat
     3841 ataatattca tcgttatagt atttgttaat gggaaattcc agggatttta aaatcacgta
     3901 agcaggcaga accgttttca tttgcccttg cacgacagat agggcaaaac acgcgagaat
     3961 ggtaattgta ttttttttca taagggggat catatttctt atcacgttaa atactatttc
     4021 tgaatcgcaa aatcaagtaa gctcattcgg tcttcaggct tataaccggg tttgccgcgg
     4081 cagctttcac cgcctggaaa ctgatcgtca tgagagcgac cgccagcgtt atccctcccg
     4141 ctacggcaaa taccggccaa cccaggtcgg tccggaaggc aaaggctgcc agccaccggt
     4201 ccatcgcata ccaggcgatc ggcacggcga tcaggatacc cagcagcacc agccgcacaa
     4261 aatccttcgc cagcagcgtg ataattccgg gcacactcgc tcccagcacc ttgcggatac
     4321 cgatctcccg gaatcgcaaa tgcgtcgcat aggcagccag agcaaaaagc cccaggcagg
     4381 agatcatgat cgctatggca gcaaaataaa agaacagggt cccctcccgc tcttcagact
     4441 ggtacagctg gttgaaagac tcgtcaagga acgcatagct gaagggatag tcggcattat
     4501 attgtttgaa aagtgccgct gcggcccgga tggcgtcggc ggcattctca cccgtggtcc
     4561 ggacatacat ccggttcagc caccagctgg gcaagctgta gaagatcgcc ggcgctatct
     4621 tctgcttcat cgatccgaag tggaaatcct ttaccactcc gacaatcgtc ccgttggtct
     4681 tcccaaggcg gaaccgcttc ccgatgggat gtgaaagccc gatctccttt atcgccgtct
     4741 cgttcagcag gaaatgcgtg gagtcggcat tcgtgccggt gaagccctcc ccttccgcca
     4801 actgcatctt gaaaaaggat agaaaatttt tgtctactgc gatgggatgg acaataaatg
     4861 tttgatccgc ccccttccca tcccagtcgt tatccccgct gatccctctc aggtcaatga
     4921 tcgtgttcgt agatgcggtc actccctgca cccccgtcaa cccgagcaat tttccctttg
     4981 ccgtttcata agcaggacgc atattccgca ttgagaagga aaagacatgg ctcctgtcat
     5041 accccaggtc cttatggcgg atatagttca gctgccggta gatcaccagg gttccgatga
     5101 tcaggatcac cgaagaagcg aattgtatca ccaccagcac cttacggaaa gcggcatcgc
     5161 ccctgctgat agtcagcttg ctcttcagcg cctttaacgg gttgaaagag gaaagcaaca
     5221 aagccgggta gatgctggac gccaccagcg tagcaccgat cgtgcaaagg atcaccgtcc
     5281 acatccgcgc gctgccgatg ttgaagacca gctgttttcc ggccagctgg ttgaacaagg
     5341 gcatcgccag ccgcaccaac accactgcca gcactgccga gaagagaaat aacaacgccg
     5401 tctccacgac gaattgcagg aagagctgga tctttgccgc gccaacgatc ttgcgcatgc
     5461 tcacctcctt gctgcgtaac atcgccctgg ccgtcgacag gttgacatag ttgatgcagg
     5521 cgatggccag tatcagcagg gcaatgatca taaaaatagt aaccgtttgc gccccggcgt
     5581 ccgatccgtc ggcattgtac aaatgcattt tattcagcgg caggagcagg taatccgcat
     5641 ccgtatcttc gggcttgttc cgcaggtgca cctggttcag cctggcgccg aggcccgcag
     5701 gagatactcc atgcttcagt tgcaggaagg tgatgtacat gtaggagttg aagtcatggt
     5761 tgagatcgat cctggattcc tgccactttt gcagcatcag cgtcatcggc atcagcatgt
     5821 cgaattggat gctcgtgttc agcgggagat cattgatcac cccgctcacg gtataattgt
     5881 cattgttatc cgtgccgatc accttgccca gcgccggctc gtccccaaag aatttccttg
     5941 ccgttgtccg cgtgatcacc accgaatgcg catcgggaaa aggcagttcc ggattccctt
     6001 ccaccagtgg gaaatcgaat agggtgaaga atgaaggatc ggtgaacatc gcccgctgat
     6061 ccgcgaacga tttatcgtgg tatttgtaga gggtggcgta attattggaa gtcatgcgca
     6121 cctgctccgt cacctccggc aactcctttt tggccagtgg ccccatcggc gccaccgtca
     6181 tactcaatat ctgtttgctt gcccccgtcc caccgaaaag ctccatgcgg tagatatccg
     6241 ccgccttctt atgaaagcta tcgaagctgc gctcgtcttc cacccaaagc aggatcagaa
     6301 tgccgatggc cagacccgcc gtgagtccgg ccatattgat cagcgaatag aatttattct
     6361 tcaccaggtt gcgccaggcg gtcttgaaat agttccgaaa catagggaaa gtttgggtag
     6421 gataaatata ttcccttttc ccgaaaaaac cgcctgttct tcaaccgatt attcaccccg
     6481 tacctgacca aactggttat tgatctgcgc aatttgtgca ttggccagat cgatgacatt
     6541 ctgcaaagcc agcttctgca tttccaaccc ggctttggta ttcgatgccg tcgccagccg
     6601 gtattccgcc aggctgacga gatattccca cacggggccg ggattgccgg gtcctactat
     6661 atggatatga cccgagccgt cgatataaac tcctcccccg tcgtttatta taccgaacaa
     6721 aatgatgagg attatgccta cgctattcag cgatggcatc tgtaaagccg gtggaatgaa
     6781 ctccgtctta aaaaagaaag ggtctatgga cgcggcgacc ggaacaccat gaagatcgga
     6841 taaggtcgct gtagcaaaat tatggtttcg ttgatagtgt accccctgcg gaaagtttgg
     6901 caaagaaggg gtattattgg cacagaatac gccatagaag gtatttccta ccgccatgat
     6961 atcggcataa tcgcctatat acgggaagaa ttggcgtatc ggcatgtcag cagggaccct
     7021 ggccagcagc gtgtcgctcc aatggacgcc atctgcagaa aacgcaaggt gcgtttccca
     7081 acgctggtgg ctatcgggac cgtcggcatc ggagcccgta aattgttgat aaagaaagcc
     7141 gatttgtccc ctgtcattta cagccaaccc gggattcttg ccattgccaa tagtcagcag
     7201 atccgggttc gaccaggtct gtcccctgtc ggtggacctg acgagatgca atttcagggc
     7261 taccggcgtt tggccggtgt cgggatcatc tggctgctcc ccccaggcaa catatacgat
     7321 cgagctattc cccggatcga cggcaatcgc caatgcgccc cccatccgct cctgagcgat
     7381 aacatcattg atcagaagtg gcacaccgat ggcaatgcgc tgaccagcca ggctatccac
     7441 accatcgatg agtgacccgt atggattggc ggactggccc caattatcat cccttacgat
     7501 cactacatcg ctggagacac tactccaatc gacacccgag tcggcccagg atttccaact
     7561 atagaagacc gcataaaccg tcccgtccct gtgaacggcc ggtcgtacct ccggcccgtt
     7621 ctggcgggga atcagatcct gaagagcgcc gaggtttttc ggccggacgt ccaggctgat
     7681 gctgctgaac acggggacag cggcccgggc gtccattgtc tgcctgataa tggatgtctg
     7741 gccggaagtc tcctcgcctt gcccaccggg cccgctgata tcattcatgc cgacatagag
     7801 cctgtctttc cctgcatccg gcccactggc aacggttgtg gcagccacat agagctgctg
     7861 cttaccacct acttgttgag aattcaatac ggtcatcact gttccggcaa ccggattcgt
     7921 tgtccgcagg atggtcgatg acgaatcgat accgctcagt acgccggcat agagctcatt
     7981 gctgctttgc ccatatttaa cggtgatacc cagtgtaccc tcgggcccgt tgcctggaac
     8041 gatacccctc agcgcccagg tatctccccc gtcgatagaa acatacagcg gcgcattggg
     8101 cccgttattg gggtccggcg tcaacaccga aataacgatc tgattggggt ttctgggatt
     8161 tacggcaata ttggggttgc tgtcctggtg ggtttccccg ctcatagcgt gtggagtgat
     8221 attgacgatc ttcatggaaa aggtctaagg ggttaaaaat agaaaggtga aattcaagga
     8281 acataaatgt agcacacccg taattaagag caaataccct tttgtgggta tgcacctccc
     8341 aatttggagc ggagaggccc ctactccttc aagaactctt tgatcgccgc caaataccgc
     8401 tccggctgct cgccatagat cccatgaccg gcgtccggga taacgacgag ccggtgatgc
     8461 ggaaagagct gcaggtattc attcgtaaat ccccatttct gattgtcgca ctgacctttc
     8521 agcacaagga tgggtatcgg agagttcctg agctttggcc gcgggtcggg caggctgcgg
     8581 cagctgaata ccgtcatgat ctggacatag aagcctgccc caccccgggg ctgtgtgtgt
     8641 tcgatcaggg acgtgtcgca gaccgtggat cgattgacct tgctgcccag gtaacaggca
     8701 aaatcgtcgg cttccctgtc ggaggcgagt tttttaccaa agctggtcgc cagcatttcc
     8761 atggtgcggg tgcgaagatt gtttgcttcc tcgttcccct ggtgattggt gtaataaggt
     8821 tcgcgcaggt gcaggctgtc cggggcaggg agctttgcga gttccggatg aaaaggtggt
     8881 agcggtccgg gaccggtaag gatcatcttt gccacacggc cgggattatc ggcggcatag
     8941 agtgcggcca ggatggcgcc ccaggactgg ccgatgagaa tcacctcctc cgcccctgtt
     9001 gcctgcacga tggcttcgag atctcttttg tggcggccgg ccgtgtactc ccggatatcg
     9061 gccagccggc cggaccagcc gctgccaatc tggtcatata catacacatc gaacccgtct
     9121 tcagaaaatg gcgtcatgat ccggatcagc ccttcactaa ccgaaccgcc cggtccaccc
     9181 tgcaaataga tgatgggata tggcttcttt acgcctttcg cggggatcag cgtaaacgcg
     9241 atacgggagc ccgtagggag atcccaataa cggatattcg cccgttcggg cattggcaac
     9301 gtgccatagg
//
LOCUS       DATGFG010000139         5644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_792941, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000139 DATGFG010000000
VERSION     DATGFG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792941"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2067
                     /locus_tag="VKQ52_02540"
     CDS             <1..2067
                     /locus_tag="VKQ52_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86085.1"
                     /translation="TVLDPSVDIWGFYNGSANGSTGLMPQLYYTNGSTNFPIYRVWDF
                     YGNRASNGQAAQANILTKITYPTGGYRQFTYEGNTVLASGDFFQYHPDPNYMAARNFN
                     QTNFSNNGSSVPAMQQLFTVNSYFGVAKLYYTLNNIGVFCSTYTVNIMQLTNPNDMTG
                     GVQLISFANSPSQNFVLANGYYRVDVYVNDYNCTMGGISGTWNECTLDNATVTTPYGT
                     YSKYVHNAGGVRVKQIQDYDPVTGNIYTTSYNYKMYSTDSTLTSGLLASPVNLLTLQN
                     DASCGCQYYKLGPGSSYPLSLDGGSYVVYPEVRTIDSAKGWTDELFTYMADIPPSTFP
                     APPPEDNSFARGRLQIQNTYTQNGTLLKKTVNNWDYGMGAYQNAVRSKAYWFHTISES
                     FTYREYPDEQNELPTMGDCTDYSVGLPPVVLGESIDSLYSPVGAQVFRNDYAYYVNNG
                     MFFLQKKIAYVNNNKTKEETYKYAFNLNSAFSFGLSSTEQSMKSTLLGKNYLLPLEVV
                     DSIRTGSGSPSFLNGSKYVFGTFNANSAIHLSQLRSYTSPVDSMVMNFTTFDGKGNLS
                     EQYKTGDVNTAYLWGYRGVYVVAKVVGSSYSTVSGLVNLAVLNNPSSDAAMQAELNKI
                     RTGLAGTAAQITTYTYSPGYGMTSSTDPSCMTTYFDYDLFGRLQDIRDQNKNIIKRYT
                     YKMNNP"
     gene            2072..2611
                     /locus_tag="VKQ52_02545"
     CDS             2072..2611
                     /locus_tag="VKQ52_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86086.1"
                     /translation="MKRYSTFLWLLLTVYAGYSQSTGGVRDLKGQSIPLDSVPSKKLL
                     IVILPAAPDTALAGQLLRFEHRHAQQVAILCILGQGAPVLAVDTNASGYGRLQSAGIW
                     LTTGMGAGDSAANIRQSVLKYLSNKSRNRQVDHFAEGSKYFLSEKGRLFGQLGKNTSL
                     DNPVADYLVQTKVPGEDRF"
     gene            2632..>5644
                     /locus_tag="VKQ52_02550"
     CDS             2632..>5644
                     /locus_tag="VKQ52_02550"
                     /inference="COORDINATES: protein motif:HMM:NF039454.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6443 domain-containing protein"
                     /protein_id="HLZ86087.1"
                     /translation="MRRLIMILLASELVITARSQDASVFTPPAYGTSLNNYIRSWEAV
                     APDTAAADFTTTTALTHSRMTTQYFDGLGRPIQTVFRNGSYPSGGSAVDLVAAQVYDA
                     YGREQRKFLPFAASSYGGNSSITDGGFKLNPFQQQQNFYSSTNGASPIYGQGETYFYS
                     KTEFEPSPLDRVVGSYAAGDNWVHGGRGLTSGYWVNTSSDSVWVWTVTNSGSPDVFDS
                     YSATSFYPSGELYKTITADENGKQIIEFKDREGNVVLKKVQLTAAVDAGSGKGPTGWL
                     CTYYVYDSVNLLRAVIQPAGVQLLQANSWNLNALNGTILKEQCFRYEYDGRRRMTMKQ
                     VPGAAVVYMVYDGRDLPVMSQDGNLRAQGKWLCTIYDAVDRPVMTALISYSGTRSQLQ
                     STVTGQTGTFGTGSVSVSGQSAATVPATLTFSTPTTGDWYASTEIDLSTNFSAGVDSD
                     FSATIVSSGSVMQSSGSVQVSNNPLPSGTSPDILTVTYYDNYNWVSGSGLGSTFEMPY
                     SPEFVTNYNASPDYAQPVTASYQTTGLATGTMARVLGTTNQYVYTVTFYDDHHRVIQT
                     RQVNYTGQTDVQTTQYSFDGKPLRVYLNHQEASTAVDKHAVMTRISYDAERRESSVWK
                     NIDGATSDQLIDSMQYDALGEVRVKLLGKDPVTGNSLDSLVYDHNIRGWMLGVNRNFV
                     GGTTKHYFGMELGYDKASSIAGTTYSNPAFNGNIAGTVWKSAGDGVDRKYDFTYDAVN
                     RFLGATYVDNESGSGWNTNKMDFSVTGLSYDANGNILTMQQKGFKVGAPTGVIDSLHY
                     GYEYGSASNKLTQVTDGANDTASLLGDFHYKGAKADSDYRYDNNGNLAMDNNKGIDSI
                     AYNYLNLPQQIHIHGKGNILYTYDAAGNKLQKQIIDSLSGLATTTLYMTGGFQYQRQA
                     PISNPSGGTDTLQFFGHEEGRARWAFHRHLAGDTAYGWEYDFMERDHLGNTRVLLTQE
                     RDTAQYMATMEPQYRATEDALFYNIDSTSYAA"
BASE COUNT         1429 a         1507 c         1426 g         1282 t
ORIGIN      
        1 accgtcctgg acccgtcggt cgatatctgg ggattttata atggttccgc caatggaagc
       61 acaggcctca tgccccaact ctactatacg aacggttcca ccaattttcc catttacaga
      121 gtctgggatt tttacggcaa ccgcgcctcc aacgggcagg ctgcccaagc aaatatcctg
      181 acgaagatca cctatcccac aggcggatat agacaattta cgtatgaagg caatacggtg
      241 ctggccagcg gggatttctt ccagtaccat cccgatccta actatatggc tgcccgcaac
      301 ttcaaccaaa ctaatttttc caataatggc tcttccgtcc ccgccatgca gcaactcttt
      361 acggtgaaca gttatttcgg cgttgccaag ctatattata cactcaataa catcggcgta
      421 ttctgctcaa cctatacagt aaatatcatg cagcttacca atccgaatga tatgaccggg
      481 ggagtacaat tgatcagctt cgccaattca ccctcgcaga atttcgtgct tgccaatggc
      541 tactaccggg tcgatgtata tgtgaatgac tacaattgta ccatgggcgg gatttccggc
      601 acctggaacg aatgtacgct ggataatgcc accgtgacta cgccttatgg cacctactcc
      661 aaatatgtgc acaatgccgg cggcgtccga gtcaaacaga tccaggacta tgaccccgtc
      721 acggggaaca tctatacgac aagctataac tataagatgt atagtacgga ctccacgctc
      781 accagcgggc tgctcgcttc acccgtcaat ctgctcaccc tgcagaacga cgcttcctgt
      841 ggttgccaat actataaatt ggggcctggc agcagctatc ctctttccct ggatggtggc
      901 tcttacgtag tctaccccga agtgcggact atcgacagtg ccaaaggttg gacggatgaa
      961 ctgtttacct acatggcaga tattcctccc agcacttttc cagcccctcc cccggaggac
     1021 aacagcttcg cccggggacg cctgcaaatc caaaatacgt acacccagaa tggcaccttg
     1081 cttaagaaga ccgtgaataa ctgggattat gggatgggtg cgtatcagaa tgccgtgcgc
     1141 tcaaaagcct actggtttca taccatttcc gagagtttta cttatcgtga atatcctgac
     1201 gagcagaatg agcttcctac tatgggagat tgtaccgatt attccgttgg tctgccgcct
     1261 gtggtcctgg gtgaaagtat cgattcgctg tattcccctg ttggagccca ggttttccga
     1321 aacgattatg cctattacgt caataatggg atgttcttcc tgcaaaagaa gattgcctac
     1381 gtcaacaaca acaagacaaa ggaagagacc tataaatatg cttttaacct caatagcgcg
     1441 ttcagttttg gcctctcctc caccgagcaa tccatgaagt ctaccctgct tggtaaaaac
     1501 tatctcttgc cactggaggt cgtcgactcc attcgaactg gcagcggcag ccccagtttt
     1561 ctaaacggtt ccaaatatgt attcggaacg ttcaatgcta acagtgccat tcacctctct
     1621 caactgcgat cctacaccag tcctgtcgat tccatggtca tgaattttac cacctttgac
     1681 ggcaagggca atctgtcgga acaatataag acaggtgacg tgaacaccgc ctatctctgg
     1741 ggatatcggg gcgtctatgt ggtagcgaaa gtcgtgggaa gcagttatag cacggtgtcc
     1801 gggttggtga acctggctgt cctaaacaac ccctccagtg acgcggcgat gcaggccgag
     1861 ttgaacaaga tccgaactgg ccttgccggc acagcggcgc aaatcacgac ttatacgtat
     1921 tcacccggct atggcatgac atcgagtacc gacccttcgt gtatgaccac ctacttcgat
     1981 tacgatctct tcggccgctt gcaggatatc cgggatcaaa ataagaacat aataaaacgg
     2041 tatacctaca aaatgaataa cccctaagga aatgaaacgc tattctacat tcttatggct
     2101 gctgctgacc gtttatgccg gctacagcca gtccaccggc ggagtaaggg acctgaaagg
     2161 acaatcgatc cccctggaca gtgttcccag taaaaagctg ctgatcgtca tcttaccagc
     2221 ggcacccgat acagcgctgg ccggccaact cctccggttt gaacacagac atgcccagca
     2281 agtggccatc ctgtgtatcc tcggccaggg ggcgccggtt ttggcggtcg acaccaatgc
     2341 cagtggctat ggccgccttc agtcggccgg catctggctc acgactggaa tgggtgccgg
     2401 cgattccgcc gcaaatattc gccaaagcgt gcttaaatac ctttcgaaca aaagcaggaa
     2461 ccggcaggtg gatcattttg cagagggcag taaatatttc ctaagtgaaa aagggcggtt
     2521 attcgggcag ttgggaaaaa atacttcgct cgacaatcct gtagccgact acctcgtgca
     2581 aacgaaagtg cccggagaag atcgattttg acatactgaa aatttgtata catgcgcaga
     2641 ttgataatga tcctattggc ttcggagttg gtgatcaccg ccagatccca ggatgcatct
     2701 gtgttcacgc cgccggcata tggtacatcc ctgaacaatt atatccgaag ctgggaagcc
     2761 gtggcgcccg ataccgcagc cgctgacttc accaccacga cggccctgac ccattcccgc
     2821 atgacgaccc aatattttga tggactcggc cggccgatcc aaacagtatt tcgaaatggc
     2881 tcttatccct ccggcggctc ggcggtagat ctggtagccg cgcaggtata tgatgcgtat
     2941 ggccgggagc agcggaaatt tctccccttc gcggccagca gctacggagg gaactcctct
     3001 attactgacg gcggctttaa gctcaatccg tttcagcaac aacagaattt ctatagcagc
     3061 accaatgggg caagtcctat ctatggccag ggagagacct acttttattc gaaaacggaa
     3121 ttcgaaccgt cgcccctcga cagggtcgtc ggcagctacg cggcgggtga caactgggta
     3181 catggtggga ggggcctgac ctccggctat tgggtgaata cttccagtga ctcagtttgg
     3241 gtctggacag taaccaacag cggctctccg gatgtcttcg acagttactc agccacttcc
     3301 ttttatccct caggagaact gtataagact attaccgcgg atgagaacgg caaacaaatt
     3361 atcgaattca aggaccggga aggcaatgtg gtgttaaaaa aagtgcagct cacggcggct
     3421 gtcgacgccg gttcagggaa agggccaacg ggctggctct gcacctatta tgtctatgac
     3481 tctgtgaact tgctcagggc cgtgatccag ccggctggtg tgcagctctt gcaagccaat
     3541 agctggaatc tcaacgcatt gaacgggacg atcctgaagg aacaatgttt ccgatacgaa
     3601 tatgacggaa gacggcggat gaccatgaag caggtgccgg gtgcagccgt tgtatatatg
     3661 gtttacgacg gccgcgacct tccggttatg agccaggacg gcaacctccg ggcacagggg
     3721 aagtggcttt gcaccattta tgacgctgtc gatcgccccg ttatgaccgc tctcatttct
     3781 tatagtggca cccggagcca gttgcagtct acggttacgg gacagaccgg taccttcggc
     3841 acgggctccg tttctgtcag cggtcaatcg gcagccactg ttccagccac actcaccttt
     3901 tccaccccca cgaccggaga ttggtatgca agtaccgaga tcgacttatc caccaacttt
     3961 tcagcggggg ttgacagtga tttcagcgcc accattgtca gtagcggttc agtcatgcag
     4021 tccagcggat cggtccaggt atcgaacaat ccattgcctt ccggcacatc tccagatatc
     4081 ctgacggtca cctattatga caattacaat tgggtaagtg gcagcgggct gggttctacg
     4141 ttcgagatgc cttattcccc cgaattcgtt accaactaca atgcctcccc tgactatgcc
     4201 cagccagtaa cggccagcta ccaaaccacc gggctggcga cgggcacaat ggcaagagtg
     4261 ttgggcacca ctaaccagta tgtatacacc gtcactttct atgacgacca tcatcgggta
     4321 atccagaccc ggcaggtaaa ttatacaggt cagaccgatg tccagacgac gcaatattca
     4381 ttcgacggca aacccctccg ggtatatctt aaccaccagg aagccagcac tgcggttgat
     4441 aagcatgcgg tcatgacgag gatatcctat gacgccgaaa gacgggaaag ctccgtgtgg
     4501 aaaaatatcg atggggcaac ctcggaccaa ctgatcgaca gtatgcaata tgatgccctt
     4561 ggggaagtaa gggtaaagct cctcggcaag gacccggtaa cgggcaatag cctcgacagc
     4621 ctggtctacg accacaatat ccgcggctgg atgctgggcg tcaatcggaa tttcgtcggt
     4681 ggtacgacga agcattattt cgggatggag ctcgggtatg ataaagcatc cagcattgcc
     4741 gggacaacct atagtaatcc cgcttttaat ggcaatattg cgggcactgt atggaagagt
     4801 gcgggcgatg gggtcgatag gaaatacgac ttcacctacg atgccgtcaa ccggttcttg
     4861 ggtgccacct atgttgacaa cgaaagcggg agcggctgga atacgaataa gatggatttt
     4921 agtgtcaccg ggctaagcta tgatgcaaat ggcaatatcc ttactatgca gcagaagggg
     4981 ttcaaggttg gggctccgac gggggtgatc gattcattgc attatggcta tgaatatggt
     5041 agcgctagca acaagctaac tcaggtgacc gatggcgcca acgatacagc gagtctgctg
     5101 ggagattttc attacaaagg cgccaaggca gattcagact accgttatga caataacggc
     5161 aatctcgcca tggacaacaa caagggaatc gacagcatag cgtataacta tctgaacctg
     5221 ccacagcaga tccacattca cgggaaaggg aatatcctct atacctatga tgcagcgggc
     5281 aacaagttgc agaagcagat catcgacagc ctatcaggtc tggcgacaac taccctgtac
     5341 atgactggtg gattccaata ccagcggcag gcgccgatca gcaatccctc cggcggcaca
     5401 gataccttgc aattcttcgg gcacgaagag ggtagggcgc gatgggcctt ccatcgacat
     5461 cttgccgggg ataccgccta cggctgggag tacgatttta tggagcggga tcacctcgga
     5521 aatacccgag tgttgctgac gcaggaacgg gatacggcgc aatacatggc gaccatggaa
     5581 ccgcaatacc gcgctactga agatgcttta ttctataaca tcgattcgac aagctatgcg
     5641 gctt
//
LOCUS       DATGFG010000140         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_319628, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000140 DATGFG010000000
VERSION     DATGFG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319628"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="VKQ52_02555"
     CDS             <1..1736
                     /locus_tag="VKQ52_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HLZ86088.1"
                     /translation="LCDRIGQLVNEKIIEGIAHINNESNKREGTRIVIDLKRDAVANI
                     VINQLYKHSELQTSYGINNVALVKGRPKTLNIRELISEFVEFRMDVVTRRTKFELKEA
                     EKKAHILQGYLIALDHLDEVIALIRASNTPEVAKENLINAGWGLDELQAKAILELRLQ
                     RLTGMERDKIRAEYDDLMTLIAYLKELLGDEGKRFEVIRKELQEVKDKFGDERKTEIT
                     YLDNEVSIKDLIREEDVVITISHLGYIKRTSATEYRQQRRGGRGAMGGRTREEDFIEH
                     LFVASTHHTMLFFTEKGRCYWLNVYEIPEGEKNSKGRAIQNLIQIPPDDKVRAIVDVK
                     DLKDEAFVNSHHIVLCTKRGTIKKTSLEEFSRPRQTGVNAITIMEGDMLLEARLTDGN
                     CEILMAIKSGRAIRFEEEKVRPTGRGAIGVAGIEVDDANDEVIGMICVNREDKTRTVL
                     VVSEKGYGKRTAIDEYRITNRGGKGVKTISVTEKTGKLVGILDVTEAEDLMITCRSGV
                     TIRMSVAGVSEQGRATQGVKLIRLDESDEIAAITKLDKLEEENNGNGILPEGAAPQDP
                     ISPDPEPAPEA"
     gene            1790..>2456
                     /locus_tag="VKQ52_02560"
     CDS             1790..>2456
                     /locus_tag="VKQ52_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86089.1"
                     /translation="MRRLFLTGFLAMVGMGLFAQSVDKANDLLKAGKITDAKTQIDGA
                     LASDKNQKIAASWYAKLKVYNAIAANDQLRAQFPDARDQAFDALKKYVDLDEKDKKLL
                     LLQMDGYKPINEIYQGYFQVGANDYNTGKYDDALKNFTGALAASGYMNTKGWTKLPID
                     TTSTLYAGISAEKANKKDTAAIYYGRLADAKIASINGTNMIDIYKWLVDYYNTRKDAA
                     NTEK"
BASE COUNT          694 a          701 c          647 g          414 t
ORIGIN      
        1 cgctttgcga ccgtatcggc caactggtca acgaaaagat cattgaaggc atcgcgcata
       61 tcaataacga atccaataag cgggaaggta cccgtatcgt catcgacctg aaaagggatg
      121 ccgtcgccaa catcgtcatc aaccagctct ataagcacag cgagctccag acctcctacg
      181 gcatcaacaa cgtagccctg gtcaaggggc ggccaaagac cctgaatatc cgggagctga
      241 tcagcgaatt cgtcgagttc cggatggatg tcgtcacccg ccggacgaaa ttcgaactga
      301 aggaagccga gaaaaaggcc catatcctcc agggctatct catcgccctg gaccatctcg
      361 acgaggtgat cgccctcatc cgggcctcca acaccccgga agtagcaaag gaaaacctca
      421 tcaacgcggg ctggggcctc gacgagctgc aggcaaaggc catcctcgaa ctgcgcctcc
      481 aacgccttac gggcatggaa cgcgacaaga tccgcgcgga atacgacgat ctgatgacac
      541 ttatcgccta cctgaaagaa ctcctgggtg acgaaggcaa acgcttcgag gtgattcgca
      601 aggagctcca ggaagtaaag gataagttcg gcgacgaacg caagaccgag atcacctatc
      661 tcgataacga ggtcagcata aaggacctga tccgggaaga agatgtggtc atcaccatct
      721 cccacctggg ctatataaaa cgcacctccg ccaccgaata ccgccagcag cggcgcggcg
      781 gccgcggcgc catgggcggt cgcacaaggg aagaggactt tatcgaacac ctcttcgtgg
      841 cctccaccca ccatacgatg ctcttcttta cggagaaggg ccggtgctac tggctcaatg
      901 tctacgagat acccgaaggc gagaagaaca gcaagggcag ggccatccag aacctgatcc
      961 agataccccc ggacgataag gttcgcgcca tcgtggatgt aaaagacctg aaggacgaag
     1021 cctttgtcaa cagccatcat atcgtgctct gtacaaaacg gggcactatc aagaagacct
     1081 ccctcgagga attcagccgc ccgcggcaga ccggcgtcaa cgccatcacc atcatggaag
     1141 gcgatatgct tctcgaagcc cggcttacgg acggcaactg cgagatcctc atggccataa
     1201 aaagcggccg ggccatccgc ttcgaggaag aaaaagtacg ccccaccggc cgcggcgcca
     1261 tcggcgtagc cggtatcgag gtcgacgatg caaatgacga ggttattggc atgatctgtg
     1321 tcaataggga agataaaacc cggacggtgc tggtggtcag cgaaaaaggg tatggtaagc
     1381 gcaccgccat cgacgaatac cggatcacca accggggagg aaagggtgta aaaaccatta
     1441 gtgttaccga gaaaaccggt aaattagtgg gcattctgga cgtcaccgag gccgaagacc
     1501 ttatgatcac ctgtagaagc ggcgtcacca tccgcatgtc ggtggccggc gtcagcgaac
     1561 aaggccgggc tacccagggt gtcaaactca tccggctgga tgagagcgat gagatcgccg
     1621 ctatcaccaa actggataaa ttggaggaag agaacaatgg taacggcatc cttcccgaag
     1681 gcgccgctcc ccaggatcct atctctcccg atcctgaacc ggccccggaa gcataagtcg
     1741 aaagacaaca gaccagagac attgtacaat taaataaaca tttaaaaaaa tgagaagact
     1801 cttcctgact ggattccttg ccatggttgg tatgggcctg ttcgctcaaa gtgtcgacaa
     1861 ggcaaatgac ctgttaaagg ccggtaagat cacggatgcc aagacgcaga tcgatggcgc
     1921 cctggcttcc gataagaacc agaagatcgc cgcatcgtgg tatgccaaat taaaggtata
     1981 caacgcgata gccgcaaacg atcagttacg tgcgcaattc ccggatgccc gcgatcaggc
     2041 attcgatgcc ctcaagaaat atgtcgacct cgacgaaaag gataagaagc tcctgctcct
     2101 ccagatggac ggctacaaac cgataaacga gatctaccag ggatatttcc aggtgggcgc
     2161 caacgactac aacaccggca agtatgacga cgccctgaag aacttcaccg gcgccctcgc
     2221 cgcctccggc tacatgaata ccaaaggctg gaccaagctc cccatcgaca ccacctcgac
     2281 gctttacgcc gggatctccg ccgaaaaagc caacaagaag gataccgccg ccatctacta
     2341 cggcaggctg gccgatgcaa agatcgcctc gatcaacggt acgaatatga tcgacatcta
     2401 caaatggctg gtagactatt acaacacaag gaaggatgcg gccaacaccg agaaat
//
LOCUS       DATGFG010000141         6921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_319622, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000141 DATGFG010000000
VERSION     DATGFG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319622"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VKQ52_02565"
     CDS             <1..919
                     /locus_tag="VKQ52_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86090.1"
                     /translation="QKGEEEKLAGAIHQVREEDPTVLLETSQELRQTIIHCQGELHLS
                     VIKWKLEHFSKNLRASFSRPRIPYRETIRKPVQSSYRHKKQSGGAGQFGEVYMQVEPW
                     FEGMPDPQGLSVRGREEFQLDWGGKLVFLNCIVGGAIDQRFLPSILKGVMEKMQEGPL
                     TGSHVRDVRVIVFDGKMHPVDSNDISFKIAGMMAFKQAFKQADPQILEPIYAVTVSCP
                     DEQTGGIISDLQTRRAILEGMSTEGHFNKIIARVPLAEMHDYYSSLRSLTQGRAKFRM
                     KFHEYAAVPSEIQRRLIDEYSRMAMHQEA"
     gene            complement(991..1650)
                     /locus_tag="VKQ52_02570"
     CDS             complement(991..1650)
                     /locus_tag="VKQ52_02570"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HLZ86091.1"
                     /translation="MPDPTLPGSIRPAAAGLSGFDVNAPLTSAEAQNFKNAGYSFCIR
                     YLPRTAGLLQNNLTNAEALVILDAGLALMAVQHVALPGWQPNTNLGTLYGNFAATYAK
                     QVVRLPPGMNIWCDLEGVAPGTPPQEVIAYCQAWYYSVHTAGYIPGIYVGYDTMLSPE
                     QLYNDISFQHYWQAYNGPDVATRGFQLIQETEKTLNGIPFDPDLTKTDNMGDSALWLS
                     L"
     gene            complement(1761..2561)
                     /locus_tag="VKQ52_02575"
     CDS             complement(1761..2561)
                     /locus_tag="VKQ52_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ86092.1"
                     /translation="MTHGRSPYTSAFYTWKGASAVFYSSCLTPFHRHSAIQLILDTQQ
                     NFRLRTKNGEWNTYRSLIIKENVVHQLDTNHSVQLIIYLEPATEIAQAISAGYLHKSE
                     VYSPQLNIFHFADSNQLQQALLRSNPSLVEALMHRILTHLSQKIGITGRDERVMRVEQ
                     MISTTAPAEISMRLLAAEVCLSESRLRALFRDVTGIPLYRYILWNKIRFATNRIMAGD
                     SVNGAAMEAGFADGSHYHKMMVQMFGMSPSTFLKENLLNDFVVCERVK"
     gene            2683..3528
                     /locus_tag="VKQ52_02580"
     CDS             2683..3528
                     /locus_tag="VKQ52_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLZ86093.1"
                     /translation="MKISLFLLSFFVVCSVLAYGQMAIPTPISRELTPVRGHYKALAS
                     ISPKPGYINKGLDSVKFPFDKAMAEEVLSLKPHYLRAVSLDDLRVPAPPANSSEQTRA
                     ELDYLLALQHNRSAEDVRSSLYMSNVHESPGDVGQTIGYWVGLQQLPMTDTLFANVSR
                     DADFFLWSIKFRYCRPRPYMLDARIHDLEESRAASYPGGHVTYAYIYAYIYQELAPQF
                     TDLFIQKAYAMAHARELIGVHYPSDSEGSRVLARTLVNLLFQNEQFLRDFEKARQEWV
                     SKKYR"
     gene            complement(3580..6042)
                     /locus_tag="VKQ52_02585"
     CDS             complement(3580..6042)
                     /locus_tag="VKQ52_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ86094.1"
                     /translation="MSDNQFRSALRFLARHKGYTGVNILGLSLGLCACLVIYNIVNYE
                     FSFDRSCPDRDRIYRIGSRIQEDNGNGALTEGYGETIPAPAPDALRAGVPLIETLAAY
                     YPYNVSITIPQAGDQNRPGATGPVRLQPTGTNPFTGTTILAGKDYFDIFPYTWLAGSP
                     AQSLSRPYSVVLTASRARDYFGDMPPSRYINRELIYDDSLHVRVSGIIRDYEGNTDFP
                     FTDFISFPTISHSFLKDTYRHDQWRFSLGVPGIQAFVKLSRNANPRQTAALFDGLLQR
                     HIATDAFLRLLKLSMVLQPLGDVHFNEAYDHDGIRKANRPALYGLIGAAVFILLLAIV
                     NFINLSTAQSLQQAKDIGIRRILGATRRRLILRSLTETAILTTLSAIIALAFLRPVMA
                     LFRNYLPEGLPFNPFSPANLLFTAGSSVVTTFLAGLYPATSLVRLQSTRTVQPQITQK
                     FTLRRVLIVFQFSISLLFIIASLVVGRQINYMLDADMGFNTGAVVTITDFDAPPQQLR
                     VFARQAAGIPGVQEYTMQGHSPAGMARMEFPMQLDNRTDNSLEVSLQAGDQRFIPFYH
                     MRLLAGRNLLSGDSLREFIINDTYRKALGFSKPADAIGHTLTWQGKTLPIVGVVADFH
                     TSSLHTPIPALTIARVANLDNSLGLRITLSNHQTLPRLETLWKSLLPDQPFTYTFLDD
                     SIARLYKRDQQLSWLVGISTVVTIFVSCIGLLGLTLFVVERKKKEISIRKVLGAGIAD
                     IVFLLNKEFMVLVGIALVVASPVAFLGMHRWLQNFAYRITISWWIFVLAGLITLTISI
                     LTISLRVIRAALINPTDNLRSE"
BASE COUNT         1592 a         1604 c         2155 g         1570 t
ORIGIN      
        1 acaaaagggt gaggaggaga aactggccgg ggccatccac caggttcggg aggaagaccc
       61 gaccgtgctg ttggagacct cgcaggagct gcggcagacg atcattcact gccaggggga
      121 attacatctg tcggtgatca aatggaagct ggagcatttc tccaaaaatc tccgcgccag
      181 tttcagccgg ccgcgtatcc cttaccggga gacgatacgt aagccggtgc agtccagcta
      241 tcgccacaaa aaacaatcgg ggggagcggg gcagttcggg gaagtatata tgcaggtgga
      301 gccctggttc gagggcatgc ccgatccgca gggtctctcg gtacgtggcc gggaggagtt
      361 ccagcttgac tggggcggga agctcgtatt cctcaactgt atcgtcggtg gcgccatcga
      421 tcaacggttc ctgccttcta tcctcaaggg tgtcatggaa aaaatgcagg aaggtccgct
      481 tacgggctcg catgtccggg atgtccgggt gatcgtgttc gatggcaaga tgcatccggt
      541 cgattccaat gatatctcct ttaagatcgc ggggatgatg gcttttaagc aggctttcaa
      601 acaggcggac ccgcagatac tcgaacccat ctatgcggtg acggtgtcct gtccggacga
      661 gcagaccggc ggcatcatca gcgacctgca gacgcgccgg gccatcctgg agggtatgag
      721 tacggagggc catttcaaca agatcatcgc aagggttccg ctggcggaaa tgcatgatta
      781 ttactcgtcg ctgcggagcc ttacgcaggg ccgggcgaag ttccggatga aattccatga
      841 atatgcggcg gtgccgtcgg agatccagcg ccggctgatc gatgaatata gccggatggc
      901 gatgcaccag gaggcgtgaa cgtccaggct gctgagcgcc gggaggttgc ggggcgccgg
      961 gaggttgatc gcgtgatgcg gcattttcga tcagagtgag agccacagag ccgagtcgcc
     1021 catattatcg gtctttgtca ggtcgggatc gaaggggatg ccattcagcg tcttttcggt
     1081 ctcctggatc agctgaaatc cgcgggtggc tacgtcgggt ccgttgtatg cctgccaata
     1141 atgctggaac gagatatcgt tatataattg ctcgggagag agcatcgtat catagcctac
     1201 atagataccc gggatatatc ccgcggtatg gaccgaataa taccaggcct gacaataggc
     1261 tattacctcc tgcggagggg tgccgggggc gacaccttcg aggtcacacc aaatattcat
     1321 gccgggtggc agccgtacta cctgttttgc gtaggttgcc gcaaaattcc catagagggt
     1381 tcccaggttg gtgttgggct gccagccggg aagtgctacg tgctgcaccg ccatcagcgc
     1441 caggcccgca tccaggataa cgagggcttc ggcattggtt agattatttt gcaggaggcc
     1501 ggccgttcgc ggcaggtagc ggatgcaaaa cgaatatcct gcattcttga aattctgtgc
     1561 ttccgcggag gtcaggggtg cattgacgtc gaatccggat aaaccggcgg ccgcgggccg
     1621 tatgcttcct gggagcgttg gatcgggcat aaacaagtgg aattaggttc aacccaattt
     1681 accgctattt gaaggttccg aggaaggggg aaaacaccta ttttccgcaa cgcgccgatc
     1741 ggtttaaagg tgctgatcgt ttatttaact ctttcacata cgacgaaatc gttcagcaga
     1801 ttctctttta ggaacgtcga ggggctcatg ccgaacatct gcaccatcat tttatggtag
     1861 tggctgccat cggcaaaccc cgcttccatc gcagcgccat tgaccgagtc tccggccata
     1921 atgcggttgg tggcgaatct tatcttgttc cacaggatat accggtataa ggggataccc
     1981 gttacgtccc ggaataaagc ccgcaacctg ctttccgaga gacagacctc cgcggcaagc
     2041 aggcgcattg aaatttccgc aggagctgtg gtcgagatca tctgttctac ccgcatgacg
     2101 cgctcgtctc tccctgtaat accaattttc tgcgataaat gggtgagtat ccgatgcatc
     2161 agggcctcca ccagggaagg attggagcgt agcaatgcct gctgcagctg gttggagtcc
     2221 gcaaaatgaa aaatattcaa ctgaggggaa tatacctcgc ttttatggag gtacccggcg
     2281 cttattgcct gagcgatctc tgttgccggc tccaggtaga tgatcaattg cacgctgtgg
     2341 ttggtgtcca actggtgaac gacattttcc tttatgatca ggcttctata ggtgttccat
     2401 tccccgttct ttgtccgaag cctgaaattt tgctgcgtgt ccaatatcag ttgtatggca
     2461 ctgtgccggt gaaaaggggt aagacaggag gaatagaaaa cggcggaggc acccttccat
     2521 gtgtaaaagg cagaagtata gggacttctt ccgtgcgtca tgacatagct tttaacaaaa
     2581 ggtagttatt atttacaact tttaagaacc ggtggtcaaa gtcagttatt tcttacaggt
     2641 tttgtttccc ggtgatggat aattttataa aaaacctggc atatgaagat ctccttattc
     2701 ctcctgtcct tctttgtcgt ctgctcggtc cttgcctacg ggcaaatggc catcccaact
     2761 cctatttccc gggagctgac acctgtccgt ggccattaca aggcactggc ctctataagt
     2821 ccaaaaccgg ggtacatcaa taaagggttg gacagcgtaa aatttccctt tgataaggcg
     2881 atggcggaag aagtgcttag cctgaagccc cattatctca gggccgtctc tttggacgat
     2941 cttcgggtac cggcgccacc tgccaacagt tcggagcaaa cccgcgccga attagactat
     3001 ctgttagcct tacaacacaa ccgatcggcg gaagatgtaa ggtcgagcct gtatatgtcg
     3061 aatgtgcatg aaagcccggg tgatgtcggg cagacgatcg gttattgggt aggcctacaa
     3121 caacttccca tgacagacac gctgttcgcc aatgtatcgc gcgatgccga tttttttctc
     3181 tggtcgataa aattcaggta ttgccgtccc aggccctaca tgctggatgc gaggatccat
     3241 gacctggagg aatcccgggc agcctcttat cccggcggtc atgtcaccta tgcttatatc
     3301 tatgcctata tttaccagga gctggccccc caattcaccg atctctttat tcaaaaagca
     3361 tatgcaatgg cgcatgcgcg cgagctgatc ggcgtgcact atcccagcga ctccgaaggt
     3421 tcgagggtat tggcaaggac actggtcaac ctgcttttcc agaatgagca attcctgcgt
     3481 gatttcgaga aggccaggca ggaatgggtg tcgaaaaaat acagataggg gtttatccct
     3541 tagggcaagt tagtgcaagg cgatatttgc aggaactttt tattctgaac ggaggttgtc
     3601 cgtaggattg atgagggccg cgcggatgac acgcaggctg atggtgagga tgctgatcgt
     3661 aagggtgatc agaccggcca gcacgaagat ccaccaggag atagtaatgc gataggcgaa
     3721 gttttgcagc cagcggtgca tgcccaggaa agcgacaggg gatgcgacga cgagggcgat
     3781 ccccaccagc accataaatt ccttattcaa taagaagacg atgtcggcaa tgccggcgcc
     3841 cagcaccttg cggatgctga tctctttctt tttgcgttcg actacgaaga gggtgagtcc
     3901 gaggaggccg atgcaggaga cgaagatggt aacaacggta gatatcccta cgagccagga
     3961 cagctgctga tcccttttgt agagccgggc gatgctgtca tcgaggaagg tataggtgaa
     4021 gggctgatcg gggaggagac ttttccagag ggtctccagc cggggcagcg tttgatggtt
     4081 gctcagcgtg atgcggaggc ctaggctgtt gtccaggttc gcgacccggg caatggtaag
     4141 ggcggggata ggcgtatgca gcgaactggt gtggaagtcc gccacgacgc cgacgatggg
     4201 aagggtcttg ccctgccagg tgagggtatg gccgatggcg tcggccggtt ttgaaaagcc
     4261 aagggctttc ctataggtgt cgttgatgat gaattctctt aagctgtcac cggacaagag
     4321 attgcggcct gccagcaggc gcatgtgata gaacgggatg aaccgctgat cgccggcctg
     4381 taggctgacc tcgagagaat tgtcggtgcg gttgtcgagt tgcatcggga attccattct
     4441 cgccatgccg gcgggggaat ggccctgcat cgtatattcc tgtacgcccg gaatgccggc
     4501 cgcctgccgg gcaaagacgc ggagctgttg cggcggggcg tcgaagtcgg tgatggtaac
     4561 cacggcgccg gtgttgaagc ccatgtcggc gtcgagcatg tagttgatct ggcggccgac
     4621 gacgagggag gcgatgatga acagtaaaga gatcgagaac tggaagacga tgaggacgcg
     4681 gcggagggtg aatttttggg tgatttgcgg ctgcacggtg cgggtggatt gcaggcggac
     4741 gaggcttgtg gccggataga gccccgcgag aaaggtggtg acgacactgc ttccggccgt
     4801 gaagaggaga ttggcggggc tgaaggggtt aaaagggagg ccttccggga ggtagttgcg
     4861 gaaaagcgcc attaccgggc ggaggaacgc cagggcgata atggcagata gcgtggtgag
     4921 gatggccgtc tccgtcagcg acctcagtat gagccggcgg cgggtggcgc cgagaatgcg
     4981 gcggatgccg atgtcctttg cctgttgcag ggactgagcc gtagacaggt tgataaagtt
     5041 gacgatcgcc agcaataaga tgaataccgc ggcgccgatg aggccgtaga gggcaggccg
     5101 gtttgccttg cggataccgt catggtcata ggcttcgttg aagtgaacgt cgccgagggg
     5161 ctgaaggacc atgctgagtt tgagcagccg caagaaggcg tcagtggcga tgtgacgttg
     5221 caataagccg tcgaacaggg ccgcagtctg cctggggttg gcgtttcttg agagttttac
     5281 gaaagcctga atgcccggaa cgccgagact gaagcgccac tggtcgtggc ggtatgtgtc
     5341 tttcaggaag ctgtggctga tggttgggaa ggagatgaag tcagtgaaag ggaagtcggt
     5401 gttgccttca tagtccctga tgatgccgga gacgcggaca tgcagggagt cgtcgtaaat
     5461 gagctcgcgg ttgatgtatc gggacggggg catatcgccg aagtagtcgc gggcgcggct
     5521 tgcggtgagt accacgctat agggacggct tagggattgg gcagggctgc cggcgagcca
     5581 ggtgtagggg aagatgtcga agtagtcctt gccggcaagg atggtggtgc cggtgaaggg
     5641 gttggtaccg gtgggctgga gccgaacggg accggtggcg ccgggtcggt tttggtcgcc
     5701 ggcttgaggg atggtaatgc tgacgttata gggataatag gcggcaaggg tttcgatgag
     5761 ggggaccccg gcacggaggg cgtcgggagc cggggcgggg atggtttcgc cgtagccttc
     5821 ggtgagggcg ccgttgccgt tgtcttcctg gatgcggctg ccgatgcgat agatccggtc
     5881 gcggtcggga caggagcggt caaagctgaa ctcgtagttg acgatattat agataaccag
     5941 gcaggcgcaa agacccaggc tcagccccag gatgttgacg ccggtatagc ccttgtgacg
     6001 ggcgaggaag cgcagggcgg agcggaactg gttgtcggac atagttctgg ttggttgcgt
     6061 agtggttgaa tgccggtggg cggccgggtt atgggccgac ggttactgcg gctgtccgtg
     6121 agatgcggga cgggaaggaa tccatagaat tcttggtaga tataaagacc aaaaagttta
     6181 tctatgggca aatttttgat taacaagtgt taagaacttg ggttgcattt tttgcggtcg
     6241 aaatttgtgc tgccttctta tgtgaatttc ttccccttac gccagataaa tcaaggcgat
     6301 ttgggtttca aacatgaaat atcacagcga ccaggaattc ccctttagag tacgacattt
     6361 ttaatggcaa gtttaaattc accactgagc ctctatggtg ctgaatatta ctacctatcc
     6421 tgtttatggc attcgtcatt gctttagtgt gggcgttaag cagatgggtg ctccctgcaa
     6481 tcggtgtcaa gtggctgtcc attattcact ggccggaatt cctgagacct agaaaagaaa
     6541 gacacccgtg atccagccca gaccagtccc accatcaatc ccaactagga taataagaaa
     6601 ggggaaagct atgcaatctt ttttgatggt tccgcagaaa ttgcaagcaa tgacgcaaaa
     6661 tatgcggaat aaaaagcgat aatatagtaa tgaaaaatat atattttcac gttgaaagac
     6721 ggcagattgt cgggtgtaat tatagaaggg cgaaccaatc cataataagg cgtatgggaa
     6781 caacctccct tggtcagtgg gcaataatgc ctcaaaaaat aggcgataat tactgaccag
     6841 gatagttaaa aattatctca atgccgtagt ggaattgatg aaagccataa aatcacggac
     6901 gaaaccgagt gcctatggat g
//
LOCUS       DATGFG010000142        10051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_431052, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000142 DATGFG010000000
VERSION     DATGFG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431052"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /gene="argS"
                     /locus_tag="VKQ52_02590"
     CDS             <1..1240
                     /gene="argS"
                     /locus_tag="VKQ52_02590"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HLZ86095.1"
                     /translation="ATPESTGIKGDHFVGDYYVRFGEEHKKQMAELLAAGKDEKTAEK
                     EAPIMKAAQQMLVDWEAGKPDVIELWKKMNSWVYAGFDETYRRIGSDFDKTYYESNTY
                     LLGKELVDQGLAKGVFVKQDDGSVIIDLTADGLDQKIVQRRDGTAVYITQDIGLAVKK
                     YEEFHSGQSIYVVGDEQNYHFKVLKLICQKLGLPSADGIFHLSYGMVELPTGRMKTRE
                     GTVVDADDILDEMIKEAERKTKELGKTDDFTLDELEDLYDIIGLGGLKFFLLRVDPKK
                     RMLFNPEESIDLHGYTATFIQYAYARIRSILRKEQFSGHPAEDPFTALLPLEKALTIT
                     LEKFPSVLEQACMEMSPSVISAYAYQLAQTYNSFYAEHSIANAETQEKKTLRLQLCQL
                     TATTLRNAMNILGIRVPERM"
     gene            complement(1293..3248)
                     /gene="glgB"
                     /locus_tag="VKQ52_02595"
     CDS             complement(1293..3248)
                     /gene="glgB"
                     /locus_tag="VKQ52_02595"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HLZ86096.1"
                     /translation="METSTRQPYEEVHFVDSTRPVWNYSLFTEEDVRNFQQGTHYRLY
                     ELFGSHRVTVLGRTGHYFAVWAPNATAVSVIGNFNDWAPDRHPLFVRLERSGIWEGFI
                     PDIPAGESYKYHIKGFNGGTLDKGDPYCNFWEMRPKTASITWELDFPWRDGEWMQVRK
                     QHNALDAPWSIYEIHLASWMRPDPNDEERYNTYDQLRELLVPYVKKMGFTHVELMPVM
                     EHPFDGSWGYQGTGYYAPTSRFGDPQGFMALVDAFHQEGIGVILDWVPSHFPYDYHGL
                     YLFDGANTYEYADMRKGFHPDWNSYIFNYSRGEVKSFLISNARFWLDKFHVDGIRVDA
                     VSSMLKLNYSRKAGGWEPNEFGGDGNLEAIAFIKDLNATVYRDFPDVQTIAEEATDWP
                     GVSRPTYAGGLGFGMKWMMGWMHDTLDYFKLDPVHRRHHQDKFTFSMMYYYDENFVLP
                     LSHDEVVHGKSPMLYKMPGDDWQKFANLRLLYTYMLTHPGGKLLFMGDEFGATTEWNY
                     KTELPWHLLQYDSHRLLQDCVRDLNGLLRGEPALYENQFTIYGFEWVDLRHRDETVIV
                     YRRKGRDAAGDLLVILNMTPVVRRDWKVYARGKEAWKEIFNSDDRKYWGTGDTYNPDI
                     PVRLVDESDGVYELTVHLPPLAGIVLK"
     gene            3382..6177
                     /locus_tag="VKQ52_02600"
     CDS             3382..6177
                     /locus_tag="VKQ52_02600"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ86097.1"
                     /translation="MPRLTALLSICLLSCMTGMAQSSHISGAIADTTEKKPLANGSVL
                     LLRPTDSILVRHTRTGSNGGFQLNVPPGHYILLVSYPSYADYVDTLFVKDTGLLRLPP
                     IGLELKSKLLETVVVSGNKGAVHIKGDTTEFNADSFRTQAGASVEDLLKKLPGIQVDK
                     NGKITAQGEAVKKVLVDGEEFFGDDPTLVTQNLRADMVDKVQVYDKKSEQATFTGIDD
                     GQREKTINLKLKNDKKMGYFGRLTAGVGTDGYYDEQLMANYFKKKEKIAAYGIISNTG
                     KTGLNWNERDTYGQSFADNLDVDQNTGSISFNSTANFDELDGWSGQYEGQGKPSVKTG
                     GLHFNNKWADDNLSLNGNYKYMQLNVAGNSETNTESILPGSVFYNNQTQNFKNQITRH
                     TADGNYEVKFDSTSSLKIQATGGADSKTTFNQYHSDFLASDSSLINQNDRTTTTAGDK
                     KIVNSYSLWRKKLPKGRTVSVAVRENFTDDKSTGWLYSKTQFYQNGASSYDSLVDQQK
                     EFHTVTTLIDSKATFTQPLSKASFLVANYGVVINNSHSNRDSYNKGYGGKYSLLDSLF
                     SNDYKYNVFTNRGGLGYTMIKKKFRFALGSDVGFTQFDQHDLVADTSGHRHFVNWYPN
                     ANFTWSITNMRRLGFNYNGSTVQPQVTQIQPIYTNENPLNITIGNPTLKPKFVSNLRV
                     FFNDYKVLTDRGIWTGINYSFTQNDIANSVTVDPNTGKQVNQYINVHGDYRVSGYFNY
                     SFKWPKPDLHFNVNANVNHNGNVSYINGLANKTTSGTYSLGTGIYKSKEKKYDFGVQF
                     NANYTESSSTINSGISTNYWTWQIQPEVNLFLPLKFQIHSDADINLRQKTPVFTTNNN
                     VMLVNAWIGKKFLKNDALLIKASVNDLLNQNVGFNRNVNSNFITQNTYNTIRRYFMFS
                     VVWNFTKAGTPMPQQRN"
     gene            6189..6959
                     /locus_tag="VKQ52_02605"
     CDS             6189..6959
                     /locus_tag="VKQ52_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HLZ86098.1"
                     /translation="MTRKLLSYALLLLLAAPGDGAWAQGNPIFLSHGRIEFQRSVNVY
                     AQMQQRMEDGDESWVDLMKKMSSHFRNSYFDLYFTGNKCLYKPGRESDDKQPFFFWQP
                     PAQDNIIFSDLEAKKGISRKNVFEEGFLIEDSLRQIKWKITDETRNIAGFACRRANAI
                     IMDSIYVVAFYTDEILAEGGPESFTGLPGMILGISLPHNHVSWFATKVETVNVTEAQV
                     APPLKGKKVNNTGLLQSVQPSMKDWGKGGRQFIETIML"
     gene            complement(6970..8886)
                     /locus_tag="VKQ52_02610"
     CDS             complement(6970..8886)
                     /locus_tag="VKQ52_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLZ86099.1"
                     /translation="MRKLFILVAGCCLVCGWVSGQPGKPNFTGGPSLADLRRGANFTG
                     SPQPQIYPTNWWVGMQHSTIQVIIRGDRVGKATGVTVQYPGVTVTGWRRAANPHYLIL
                     HMRIGAEAKPGTVTIGLTGVGSVDFGLETRRKGNGTAYAQGVTAADFIYFLMPDRWSN
                     GDPGNDRVPGMRDQSLNRDSIFLRHGGDLQGVLNHLDYLQQLGVTALWMTPVIENDMP
                     DRTEHGYAFTDHYTIEPRLGGAAMYRKLSDALHQRGMKLIQDAVYNHVGLYHWLVQDP
                     PDSDWLHRWPVYTQTNYRGEPVMDPHASAGDRKLSSDGWFTREMPDLNQDNPGLAEFL
                     IQHAIWCVESFGVDAWRIDTYMYNSLSFMNRCNEALLKEYPKLTMFGEAWIMGTAKQA
                     YFARNNLVVPFKSNLPGVLDFQCLFNGIAPAVKDTSAGGGGLNRLYQTLSDDFLYQDP
                     SANVIFLDNHDMTRWFSQTGEDVAAQRMGLEWLLTERGIPQLYYGTEIMTKGVDNPDG
                     LVRLDFPGGWAGDERSAFTGAGLGADEKSIQDLVKKLGQFRLHSSALRYGRMMQYVPT
                     GPLYVYFRFDANQTILCAMNTANGPVTVDFARFSERTKGFKEGVDVVTGERHPLDQPA
                     RLPGRTMWVLELQR"
     gene            complement(8898..>10051)
                     /locus_tag="VKQ52_02615"
     CDS             complement(8898..>10051)
                     /locus_tag="VKQ52_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ86100.1"
                     /translation="GRRKPYFLTGALLSAIALILLPNSGAMAGLIPALWIGAIFVMVM
                     DASFNVAMEPFRALVADNLPDSQRAKGFAIQAFLIGVGAVGGSYMTSLLAWLGFSQAP
                     GPNGIADNIKYSFYIGATIFILAILVTMFFSKEYPPEEYERYHGHAEEKKGKAGLGEI
                     GRDFLKMPKTMKQLGLVQFFSWFAMFSMWVFTTDAVASHVFGLQGDYAHTKAYNQAGD
                     IVGNAFAIYNGVGALYALCLTPMARRLGRKMTHAISLLAGGIGLISIFFAHGAWGLYF
                     AMVGVGLAWASILAMPYVILSGCIPAGKMGIYMGIFNFFITLPQIVNGLFGGWIVKHL
                     YGNQSIYSIVLAGFCLIAAAISVLFVYDGGAIRIQQEKAGFYAAERTAS"
BASE COUNT         2469 a         3102 c         2485 g         1995 t
ORIGIN      
        1 cgccaccccc gaaagcaccg gcatcaaggg cgaccacttc gtcggcgact actatgtccg
       61 ctttggcgag gaacacaaaa aacagatggc cgaactcctc gctgccggca aggacgagaa
      121 gacggccgaa aaagaagcgc ccatcatgaa ggccgcccag caaatgctcg tcgactggga
      181 agccggaaaa cccgatgtca tcgaactctg gaagaaaatg aatagctggg tctacgccgg
      241 cttcgatgaa acgtatcgcc gcatcggcag cgacttcgac aagacctact acgaaagcaa
      301 tacctacctg ctcggcaagg aactggtcga ccagggcctg gcaaaagggg tttttgtcaa
      361 acaggacgac ggcagcgtga tcatcgatct gacggccgac ggtctcgatc agaagatcgt
      421 tcagcgccgg gacgggacgg cggtctatat cacccaggac atcggcctgg ccgtaaaaaa
      481 atacgaggag tttcactccg gccaaagcat ctatgtggtc ggcgacgaac agaactatca
      541 cttcaaggtg ctaaagctca tctgccagaa actgggtctg ccttccgccg acggtatctt
      601 ccatctcagc tacggcatgg tcgaactccc cacgggccgg atgaagaccc gggaagggac
      661 cgtggtcgac gcggatgata tcctcgatga gatgataaaa gaagcggaac gaaaaacaaa
      721 ggaactgggg aagacggacg atttcacgct cgacgaactg gaagacctgt acgatatcat
      781 cggtctcggc ggcctgaaat tcttcctcct gcgcgtggac cccaaaaaga ggatgctctt
      841 caacccggaa gaatccatcg atctgcatgg gtataccgct acctttatcc aatatgccta
      901 tgcgcggatc cgcagcatcc tgcggaaaga gcagttctcc ggccatcccg ccgaggaccc
      961 gtttaccgcc ctcctgcccc tcgaaaaagc gctgacgata accctggaga aattcccgtc
     1021 cgtcctggaa caggcctgca tggaaatgag cccttcggtg atctccgcct atgcctacca
     1081 gttagcgcag acctataatt ctttttatgc ggagcacagc atcgccaatg ccgaaacgca
     1141 ggagaagaag acccttcgcc tccagctctg ccaactgacg gccaccaccc tgcgcaacgc
     1201 gatgaacatc ctcggcatcc gcgtcccgga aaggatgtag gccggcgaag cggaacggat
     1261 gctggccggc ggcccggccc gcccgtcgta gcttatttca ggacgatccc ggccagcggc
     1321 ggcagatgga cggtcagttc gtagacccca tcactctcat ccaccagtcg cacgggaata
     1381 tcggggttat aggtatcccc cgtcccccag tatttccggt catcgctgtt gaagatctcc
     1441 ttccacgcct ccttgccccg cgcatagacc ttccagtccc ggcgcacgac aggcgtcata
     1501 ttgagaatga ccaggagatc tcccgccgca tcccggccct tccggcgata gacgatcacc
     1561 gtctcgtccc ggtgacggag gtccacccac tcgaacccat aaatagtgaa ctggttctca
     1621 tataaagccg gctctccccg caggaggccg ttgagatcgc gcacacaatc ctgcaacagg
     1681 cggtgcgaat catattgcaa caggtgccac ggtagctccg ttttatagtt ccactccgtc
     1741 gtcgccccga actcatcgcc catgaacaac agtttcccgc ccgggtgcgt cagcatatag
     1801 gtatacaaaa gccgcaggtt ggcgaacttc tgccagtcat ctcccggcat cttatagagc
     1861 atcggcgact taccatgcac cacctcgtca tgactcaacg gcaatacaaa attctcgtca
     1921 tagtaataca tcatgctaaa ggtgaacttg tcctggtgat gccggcggtg cacggggtcc
     1981 agcttgaaat agtccagcgt gtcatgcatc cagcccatca tccatttcat cccgaaacca
     2041 agacctcccg cataggtggg ccggcttact cccggccagt ccgtagcctc ctcggcaatg
     2101 gtctgcacat cggggaaatc ccggtacacc gtggcattga ggtccttgat aaaggcgatc
     2161 gcttccaggt tgccatcgcc cccgaactca ttgggctccc agcctcccgc cttccgcgaa
     2221 tagttcagct tcagcatcga actcaccgcg tcaaccctga tcccatccac atggaacttg
     2281 tccagccaga accgtgcgtt gctgatcagg aaggatttca cctccccccg gctgtaattg
     2341 aagatatagc tgttccagtc ggggtggaac ccctttcgca tatcggcata ctcataggta
     2401 ttggcgccgt cgaagagata aagtccgtga tagtcgtacg ggaaatgcga gggcacccag
     2461 tccaggatca ccccgatgcc ttcctggtga aaggcatcca ccagcgccat aaagccctgg
     2521 gggtcgccaa accgcgaagt aggcgcataa taccccgttc cctggtagcc ccagctcccg
     2581 tcgaacggat gctccatcac gggcatcagc tccacatgcg tgaaccccat cttcttcaca
     2641 tacgggacca gcagctcccg caactggtca taggtgttgt accgctcttc gtcattgggg
     2701 tccggccgca tccaggacgc caggtggatc tcgtaaatac tccagggcgc atccagggca
     2761 ttatgctgct ttcgcacctg catccactcc ccgtcccgcc agggaaaatc cagctcccag
     2821 gtgatggatg ccgtcttggg ccgcatctcc cagaaattac aatatggatc acccttgtcc
     2881 agggtcccgc cattaaaccc cttgatatga tacttatacg attcaccggc aggaatatcc
     2941 gggataaagc cttcccagat gcccgaacgc tccagccgca cgaagagagg atgccggtcg
     3001 ggtgcccagt cattgaaatt gccgatcacg gacaccgcgg tcgcattggg cgcccagaca
     3061 gcaaagtaat gccccgtcct gccaagcacc gtcacccggt gactgccgaa caattcataa
     3121 agccggtaat gtgtaccctg ttgaaaatta cgaacgtcct cctcggtaaa cagagaataa
     3181 ttccacaccg gtcgagtgga atccacgaaa tgcacttcct catacggctg gcgggttgat
     3241 gtttccaaga tttttttttt gtgatgtatg gaatgcaaca gttttgtact cctactgtct
     3301 ttaacgtaaa tttaagggat cgcccttgca tatccttcat tttgaatcta atttcaggca
     3361 cctaatcgag aaaacatcct tatgcctaga ctgactgccc ttctgtccat atgcctgttg
     3421 tcttgcatga ccgggatggc ccagtcttca catatttccg gcgcgatcgc cgacaccacc
     3481 gagaaaaagc cgcttgccaa tggctccgtt ctcctccttc gacccaccga cagcatcctc
     3541 gtccgtcata cccgtaccgg ttccaacggc ggttttcaac tgaacgtacc ccccggtcac
     3601 tatatcctcc tggtcagcta cccctcctat gccgactatg tcgatacact cttcgtaaaa
     3661 gataccggcc tcctccgcct cccccccatc gggctggaac tgaaaagcaa actcctcgag
     3721 accgtcgtcg tcagcggcaa taaaggcgcc gtccacatca agggcgatac caccgaattc
     3781 aacgccgaca gcttccgcac ccaggccggc gcctccgtcg aagacctcct gaagaaactc
     3841 ccaggtatcc aggtggacaa gaacggaaag atcaccgccc agggcgaagc cgtaaaaaag
     3901 gtactcgtcg acggcgagga gttctttggc gacgacccca ccctcgtgac gcaaaacctg
     3961 cgcgccgata tggtcgataa agtacaggtc tatgataaga aaagcgaaca ggccaccttc
     4021 acgggcattg acgacggaca gcgcgaaaag accatcaacc tgaaactgaa gaacgataag
     4081 aagatggggt acttcgggcg gctgaccgcc ggcgtgggca ccgacggcta ttatgacgaa
     4141 cagctcatgg ccaattactt taagaaaaaa gagaagatcg ccgcctatgg gatcatctcc
     4201 aacaccggca agaccggtct caactggaac gaacgcgaca cctatggcca gagcttcgcg
     4261 gacaacctgg atgtcgatca gaacaccggc tccataagct ttaattcgac ggccaatttt
     4321 gacgaactcg acggctggtc gggccaatac gaaggccagg gcaaacccag cgtaaagacc
     4381 ggcggactgc atttcaataa taaatgggcg gacgacaacc tctccctcaa cggcaactac
     4441 aagtatatgc agttgaatgt cgcgggcaac agcgaaacca ataccgagag catcctgccg
     4501 ggttctgtct tttacaacaa ccagacccag aatttcaaaa accagatcac gcgtcatact
     4561 gccgacggca actacgaagt gaagttcgat tccacctcct cactgaagat ccaggccacc
     4621 ggcggcgccg atagcaagac cactttcaat cagtatcact cggattttct cgccagcgac
     4681 agcagtctga tcaatcagaa cgaccggaca accaccaccg ccggcgacaa gaaaatagta
     4741 aacagctact cgctctggcg aaagaagctg ccaaaaggcc gaacggtgtc cgtggccgtc
     4801 cgcgagaatt tcaccgatga caaatccacc ggctggctat actccaagac ccaattctat
     4861 cagaatggag catcctccta cgactcgctg gtcgaccagc aaaaggagtt ccatacggtt
     4921 actaccctca tcgattccaa agcgacattc acccagcctc tgtcaaaagc ctccttcctc
     4981 gtggccaact acggtgtggt gatcaataac agccattcga accgcgattc gtataacaag
     5041 ggctatggcg gcaagtacag ccttctcgat tccctcttca gcaacgacta taaatacaat
     5101 gtattcacca acagaggcgg tcttgggtat accatgataa aaaagaagtt ccggtttgcc
     5161 ctgggttccg atgtcgggtt cacccagttc gatcagcacg acctggtggc ggataccagc
     5221 ggtcaccgcc atttcgtgaa ttggtaccct aatgccaact tcacctggtc gatcaccaat
     5281 atgcggcgtc tgggcttcaa ttataacggc agcaccgtcc aaccccaggt aacccagatc
     5341 cagcccatct ataccaatga gaacccgctg aatatcacca tcggcaatcc taccctcaag
     5401 cccaaattcg tcagcaatct cagggtgttc ttcaacgatt ataaggtgct cacggatcgc
     5461 ggcatctgga cgggcatcaa ctattccttt acccagaatg acatcgccaa cagcgttacc
     5521 gttgacccca acaccggtaa acaggtgaac cagtacatca acgtacacgg tgactaccgg
     5581 gtcagcggtt atttcaacta ttccttcaaa tggccaaaac cagatctgca ttttaatgtc
     5641 aatgccaacg tcaatcataa tggtaatgtc agctatatca atggactggc caataagaca
     5701 accagcggca cctatagtct tggcaccggt atctataaat cgaaggaaaa gaaatacgac
     5761 ttcggggttc agttcaacgc aaactacacc gagagctcga gcacgatcaa cagcgggatc
     5821 tccaccaact actggacctg gcagatccaa cccgaagtca acctgttcct gccgctgaag
     5881 ttccagatcc actcggatgc cgatatcaac cttcgccaga aaacgccggt cttcaccacc
     5941 aacaacaacg tgatgctggt caatgcctgg atcggcaaga agttcctgaa gaacgatgcc
     6001 ctgctgatca aggcctccgt caatgatctg ctcaaccaga acgtcggctt caaccgcaat
     6061 gtgaacagca acttcatcac ccagaacact tataacacga tcaggcgcta ctttatgttc
     6121 tccgtagtct ggaattttac aaaggcagga acacccatgc cgcaacaaag aaattaatgc
     6181 atcacgatat gacacgcaaa ctcctttctt acgctctttt gctgcttctc gcggcccccg
     6241 gcgatggggc ctgggcgcag ggaaatccca tttttctctc gcatggccgt atcgaatttc
     6301 aacgcagcgt gaatgtctac gcccagatgc agcagcggat ggaagacggc gatgaaagtt
     6361 gggtcgatct gatgaaaaaa atgtccagcc atttccggaa ctcctatttc gatctctatt
     6421 tcaccggcaa caaatgcctg tataagcccg gccgcgagag cgacgacaag cagcccttct
     6481 ttttctggca gccacccgcc caggataata tcatcttctc cgatctggaa gcaaaaaagg
     6541 gcatcagccg gaagaatgtg tttgaagagg gattcctgat agaggacagt ctccggcaga
     6601 taaaatggaa gatcacagac gagacccgga atatcgccgg cttcgcctgc cgccgcgcga
     6661 atgccattat catggactcg atctatgtgg tagccttcta caccgatgag atcctggcgg
     6721 aaggcggacc ggagtccttt accggcttgc cgggaatgat cctcggaatt tcccttcctc
     6781 acaatcatgt ctcctggttt gctacaaagg tggagaccgt caatgtcacc gaagcccagg
     6841 tggcaccccc gctaaaaggt aagaaggtca acaacaccgg actcctgcaa agcgtccagc
     6901 ccagtatgaa agattgggga aaaggagggc gacagtttat agaaacgata atgctgtagt
     6961 cgccgtgtct taccgttgca gctccagcac ccacatggtc cggccgggca gtcgcgcggg
     7021 ctggtcgagc ggatgccgct cccccgtcac cacatcgaca ccctccttaa aacccttcgt
     7081 ccgttcgcta aaccgcgcaa aatccaccgt taccgggcca ttcgccgtat tcatcgcgca
     7141 aaggatcgtt tgattcgcat caaaccggaa atagacatag agcggacccg tcggcacata
     7201 ttgcatcatc ctgccatagc gcagcgccga cgaatgcagc cgaaactgcc ccagcttctt
     7261 caccagatcc tgtatactct tctcgtcggc ccccaggccc gcgccggtga atgcactgcg
     7321 ttcatcaccc gcccacccgc cggggaaatc gagccgcaca agcccatccg ggttatcgac
     7381 cccctttgtc atgatctccg ttccataata gagctggggg atgccgcgtt cggtaagcag
     7441 ccattccagc cccatccgct gggccgccac gtcttccccg gtttgcgaga accatctcgt
     7501 catatcatgg ttgtcgagaa agataacatt ggccgagggg tcctggtaga ggaagtcatc
     7561 gctcagggtc tggtacaacc ggttaagacc gccgccgccg gcggaagtat ccttcaccgc
     7621 gggggcgatc ccattgaaaa gacactggaa atccagcacc ccggggagat tgcttttgaa
     7681 agggacgaca aggttattcc gcgcgaaata ggcctgtttg gccgtgccca tgatccaggc
     7741 ctcgccaaac atcgtcagct tcgggtattc cttcagcagg gcctcattac accggttcat
     7801 aaacgacagg ctgttgtaca tataggtgtc gatccgccag gcgtcgaccc caaagctctc
     7861 cacacaccag atggcatgct ggatcaaaaa ctcggctaac ccggggttgt cctggttgag
     7921 atcgggcatc tcccgcgtga accagccatc gctcgacaat ttccggtcgc cggcactggc
     7981 atgcgggtcc atcacgggtt ctccgcggta gttcgtctgt gtatacacgg gccaccggtg
     8041 caaccagtcc gaatcgggcg ggtcctgaac aagccagtga taaagcccga catggttgta
     8101 caccgcatcc tggatcagct tcatcccccg ctggtgcagg gcatccgaca gtttccggta
     8161 catcgccgcg ccgccaagcc gcggctcgat ggtataatga tcggtgaaag catacccgtg
     8221 ttccgtcctg tcgggcatat cgttctcgat caccggcgtc atccacaacg ccgtcacccc
     8281 cagctgctgc aggtaatcga ggtggttgag gacaccctgg agatcgccgc catgccgcag
     8341 gaaaatagaa tccctgttaa gggactggtc gcgcatcccg ggtacccggt cattgcccgg
     8401 atcgccatta ctccagcggt ccggcatcag gaaataaatg aagtcggccg cggtgactcc
     8461 ctgcgcatag gccgtcccgt tacccttccg ccttgtctcc agcccaaaat cgacgctccc
     8521 cacgccggtc agcccgatgg tcacggtccc gggcttcgcc tccgccccaa ttcgcatatg
     8581 caggatcagg taatggggat tagccgcccg gcgccagcct gtcaccgtca ccccggggta
     8641 ctgtactgtt acccccgtcg ccttccctac cctgtcgcca cggatgatca cctgtatcgt
     8701 ggaatgttgc attcccaccc accaattcgt aggatagatt tgcggctggg ggcttccggt
     8761 aaaattagcc cctcggcgca aatccgccaa tgatgggcct ccggtaaaat tgggtttccc
     8821 gggctgtccg gatacccacc cgcacaccag gcaacagccg gcgacaagaa tgaacaactt
     8881 tcgcataatg gatgaattta agaggccgtg cgctccgccg catagaatcc ggctttctcc
     8941 tgctggatgc ggatagcgcc gccatcatag acgaatagca ccgaaatcgc cgcggcgatc
     9001 aggcagaacc cggccaggac gatggaatag atcgactggt tgccataaag atgtttgacg
     9061 atccagccgc caaaaagtcc gttcacgatc tgcggcaggg tgatgaagaa gttgaagatc
     9121 cccatataga tccccatctt gcccgcgggg atacaaccgc tcaggatcac gtagggcatc
     9181 gccaggatgc tcgcccaggc cagcccaacg ccaaccatcg caaaatataa gccccatgcc
     9241 ccatgcgcaa agaagatcga gatcagcccg atacccccgg ctaacaggga aatcgcatgt
     9301 gtcatcttcc ggcccagcct ccgcgccatc ggcgtcaggc agagcgcata gagggccccc
     9361 accccattgt agatcgcaaa ggcgttaccg acgatatccc cggcctggtt ataggccttc
     9421 gtatgcgcat agtccccctg taacccgaat acatgcgagg ccacggcgtc ggtggtgaat
     9481 acccacatgc tgaacatcgc aaaccaactg aagaactgca ccagcccaag ctgcttcatc
     9541 gtcttcggca ttttcaaaaa atcccggccg atctctccca gcccggcctt ccctttcttt
     9601 tcctccgcat ggccatggta acgctcgtat tcctccggcg gatattcctt cgaaaagaac
     9661 atagtcacga ggatggccag gataaagatc gtggcgccga tgtaaaagga gtatttgata
     9721 ttgtccgcga tcccgttggg cccgggcgcc tggctaaacc ccagccaggc aagcaggctg
     9781 gtcatataag acccgcccac ggcgccgacg ccgatgagaa aggcctggat ggcaaaaccc
     9841 ttggcccgct ggctgtccgg caggttatcc gccaccagtg cccggaaagg ctccatcgca
     9901 acattaaagg aggcgtccat caccatgaca aaaatggcgc cgatccacaa cgcagggatc
     9961 agcccggcca tggccccgga attgggcagc aggatcagcg caatggcaga taaaagggcg
    10021 cccgtgagaa aatacggttt gcgccttccc a
//
LOCUS       DATGFG010000143         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_795357, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000143 DATGFG010000000
VERSION     DATGFG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795357"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VKQ52_02620"
     CDS             complement(<1..676)
                     /locus_tag="VKQ52_02620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ86101.1"
                     /translation="MAPQTPLLTIIMPTFGAEKTLQESLDSVLRQDFRDWELLIMDGG
                     STDGTLGLARGAAGKDTRIRVFSEKDKGVYDAMNKGIHQARGEWLYFLGSDDQLHDDK
                     VLSTVLSMPDTVRYDLLYGNVVGASYKGVYDGVFDYTKLLGKNLSHQAAFYRLTLFRR
                     LGDYDLRYKAYADWEFNIRCFSAEGVRIRYIDVIVARFGADGLSSRHDVPFLKQVLFP
                     AKLRLLP"
     gene            complement(652..1578)
                     /locus_tag="VKQ52_02625"
     CDS             complement(652..1578)
                     /locus_tag="VKQ52_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86102.1"
                     /translation="MSVVLTNLSNRLYGESRLRLNASAKRWGIEEVRSYDWEELASTA
                     FYRDNRGILEQPTGMGYWLWKPYIILEALNAIPDGDIVVYADSGVEIIASLDPLFGIC
                     CQENPILLFGNGDFPNSMWTKRDCFILMDCDREAYWKAPQCDAAFCIFQRCETSIRFV
                     TEWLNYCRDARILTDSPNTCGRRNLPDFIAHRRDQSVLSLMAAKYRLPLFRMPTQFGN
                     HYKTYACREEGEFNCVNQYRQRQVGYYAPIPYYNSPYFQLLDHHRGQNGDGGGTVKKN
                     NREPFSFVLRIVRKRYTRWLNTFDLWRHKHHS"
     gene            complement(1580..>2257)
                     /locus_tag="VKQ52_02630"
     CDS             complement(1580..>2257)
                     /locus_tag="VKQ52_02630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ86103.1"
                     /translation="FARSYFQYGVPGFLKPVINKPIGNAPTYSDVMALLARRYLQRVN
                     YLEIGVSVGKNFFQMLNALDNAQFSAFDIEEINPVLAKKLSPGPRLEWDTPTGSIKKN
                     KSSLSSYTYRDKKVGYLSGDVWDVNSWSKLEGNRFNLVFSDALHTPKAILFEFEMLVK
                     YRLLDDRFVIVWDDLVGKMKNSFFKIIRKYNKVYQIKDIYLLEINGWVGENEGPHSVG
                     IISNFAF"
BASE COUNT          527 a          631 c          605 g          494 t
ORIGIN      
        1 ccggcagcaa ccgaagcttc gcggggaaca gcacctgctt aaggaagggg acatcgtgcc
       61 gggaactgag accgtcggcg ccaaaacggg cgacgatgac atcgatatac cggatgcgga
      121 ccccttcagc cgaaaagcag cggatattga attcccagtc ggcataggct ttataacgca
      181 gatcataatc ccccaaccgc cggaacagcg tcagccggta aaacgcggcc tggtgggaaa
      241 ggttcttacc aagcagtttg gtatagtcga atacgccgtc atacaccccc ttgtaactgg
      301 cgccgacgac gttgccatat agcagatcat accggaccgt atccggcatc gaaagaacgg
      361 tactcaatac cttgtcatcg tgcagctggt cgtcgctgcc aagaaagtac agccactccc
      421 cacgggcctg gtgtatcccc ttattcatcg catcgtagac gcccttgtcc ttttcggaga
      481 agaccctgat gcgcgtatcc ttcccggccg ccccccgggc aaggccaagg gtcccgtctg
      541 tcgacccgcc atccatgatg agcagctccc aatcccggaa atcctggcgc aatacgctgt
      601 ccaaagactc ctgcagtgtc ttttcggccc cgaaagtggg catgataatc gtcaggagtg
      661 gtgtttgtgg cgccatagat cgaaggtgtt caaccaacgc gtgtatcgct tgcgaacgat
      721 ccgcagaaca aaagagaagg gctcccggtt gttttttttg accgtaccgc ctccatcccc
      781 gttctgtccg cgatgatgat ccagcaattg aaaatagggt gaattataat agggtatcgg
      841 cgcatagtaa cctacctgcc gttgccggta ctggttgacg caattgaact cgccctcctc
      901 gcggcaggca taggtcttgt agtggttgcc aaactgggta ggcatccgga acaacggaag
      961 ccggtatttc gccgccatca gcgagagcac ggactggtcc ctccggtggg caataaagtc
     1021 gggaagattg cggcggccgc aggtattggg gctgtccgtg aggatacggg catcccggca
     1081 atagttcagc cattccgtaa cgaaccggat actggtctca cagcgctgga agatacagaa
     1141 ggcggcatca cactgcgggg ctttccagta ggcttcccgg tcgcaatcca tcaggataaa
     1201 acagtcccgc tttgtccaca tggaattggg aaaatccccg ttgccgaaaa gaaggatggg
     1261 attctcctgg cagcagatgc cgaacagtgg gtccagcgac gctatgatct ccacgccgct
     1321 gtccgcgtaa acgacgatat ccccatcggg gatggcgttc aatgcttcga ggatgatgta
     1381 gggcttccag agccaatagc ccattcccgt cggctgttcc agtatcccgc gattatcccg
     1441 gtaaaaagca gtggacgcga gttcttccca atcgtatgac cgcacctcct ctatccccca
     1501 tcttttcgcc gatgcgttca ggcggagccg gctctcacca tacaggcggt tggaaagatt
     1561 ggtcagtacg acggacatgc tagaaggcga aatttgagat gatgcctacc gaatgcggac
     1621 cctcgttctc ccccacccag ccatttatct ccagcaggta gatatccttt atctggtaga
     1681 ctttattgta tttccggatg atcttgaaaa aggaattctt catcttgccg acgagatcgt
     1741 cccagacaat gacaaacctg tcatcgagca gccggtattt gaccagcatc tcgaattcga
     1801 acaagatggc ctttggcgta tggagggcgt ccgagaaaac caggttgaac cggttgcctt
     1861 ccagcttcga ccagctgttg acatcccaga cgtctcccga gagatagccg acctttttgt
     1921 ccctgtaggt atagctgctg agggagctct tgttcttctt gatggaaccg gtgggtgtat
     1981 cccattccag ccggggcccc ggcgacaatt ttttggccag cacgggattg atctcttcga
     2041 tgtcgaaagc cgagaactgg gcattatcca gcgcattcag catctggaag aagttcttgc
     2101 cgaccgagac gccgatctcg aggtaattca ctcgctggag ataacgcctg gccagcaggg
     2161 ccatcacatc ggaatacgtg ggtgcattcc cgatgggttt attgatcacg ggctttaaga
     2221 aacccggtac gccatattga aaataagaac gcgcaaa
//
LOCUS       DATGFG010000144         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_547146, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000144 DATGFG010000000
VERSION     DATGFG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547146"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2350
                     /gene="mfd"
                     /locus_tag="VKQ52_02635"
     CDS             <1..2350
                     /gene="mfd"
                     /locus_tag="VKQ52_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HLZ86104.1"
                     /translation="LIRDLKTLEAKKYTLFLFAENPRQLERLHSIFQDLKAEIPFTPI
                     AHSIHEGFIDEDLKIACFTDHQIFQRYHKYKVKQAYNKNKAITLRTLRELQPGDYVTH
                     IDHGVGVYSGLQKIEANGRLQEAVRIIYKDSDILYVNINSLHKISKYTGKEGTVPKVN
                     KLGSDAWQKLKEKTKTKVKEIAFDLIKLYAQRKAEIGFQHSPDNYMQTELEASFIYED
                     TPDQGKATADVKKDMEAPSPMDRLVCGDVGFGKTEVAIRAAFKTCCDGKQAAILVPTT
                     ILAFQHYKTFSDRLKDFPVKVDFVNRFKSAKEKKETLKQLEAGQTEIIIGTHALLGKE
                     VKFKNLGLLVIDEEQKFGVAHKEKIKTLRTHVDCLTLTATPIPRTLQFSLMGARDLSI
                     INTPPPNRQPIQTELHGYNEDFIRDAIYYETERGGQVFFIYNRVQGLAEMSALIQGLC
                     PDLSIGYAHGQMEGNDLENRILSFIDRKFDVLICTNIVESGVDIPNVNTIIVNNAHHF
                     GLSDLHQLRGRVGRGNRKAFCYLLAPSLATLPDDSRKRLQTLEQHSDLGSGFQIAMRD
                     LDIRGAGNMLGGEQSGFMVDIGFETYQKILDEAIKELKRTEFRELFKEEISKQEDYVQ
                     DCTIDTDLEILIPDSYVESITERLSLYTRLDNCDSEDELQQFHAELIDRFGPIPPQVE
                     DLFDTVRIRKLAVSLGFEKLILKEDTMRCYFIDRPDSPYFESETFHLILEYLQKQTNK
                     ARLKQAGKHFLLVVDDVRSMADLLGFLQRMSKFALAPRALMG"
     gene            2427..2780
                     /locus_tag="VKQ52_02640"
     CDS             2427..2780
                     /locus_tag="VKQ52_02640"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HLZ86105.1"
                     /translation="MPEEYPLTTPTSDERTLGILSHILAICPGIGIFGPLVIWLIKKD
                     ESQFVSENAKESLNFQLTMIIAFIISGILMVVLIGFFLFWILGILNIILVIIATIKAS
                     ENKIYRYPFNLRLIK"
     gene            complement(2787..3239)
                     /locus_tag="VKQ52_02645"
     CDS             complement(2787..3239)
                     /locus_tag="VKQ52_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ86106.1"
                     /translation="MYRKYNNSMALRMIDHGTKEYQQMVNLRNEILRKPLGLLFTKEE
                     LDREKDDILMGAFEDDRLLGCCMLSRMDAITVRLRQMAVPNNMQGKGIGRALMIFAEN
                     IARDLGYKKLCMHARKTAVGFYQKLGYGITGDEFVEVTIPHYIMEKAL"
     gene            3380..3616
                     /locus_tag="VKQ52_02650"
     CDS             3380..3616
                     /locus_tag="VKQ52_02650"
                     /inference="COORDINATES: protein motif:HMM:NF002148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLZ86107.1"
                     /translation="MSDIATRVKKIIVDKLGVEEAEVTNEASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDEQAETITTVGQAIAYLEEHAK"
     gene            3830..>4796
                     /locus_tag="VKQ52_02655"
     CDS             3830..>4796
                     /locus_tag="VKQ52_02655"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLZ86108.1"
                     /translation="MQLKRVVVTGIGALTPLGNNTQDYWKGLLNGVSGAGSITLFDAA
                     KFKTRFACELKGFDPLNYLDRKEARKVDRYTQIALAASDEAVKDAAIDKDTMNPDRIG
                     VVFASGIGGLITFQEEVINFAKGDGTPRFNPFFIPKMILDIAAGHISMRHGFRGPNFA
                     VTSACASSTNAIMEAFNLIRLGMADILISGGSEAVVSEAGVGGFNAMKALSERNDDPA
                     TASRPYDKDRDGFVMGEAAGVLVLEELEHAKARGARIYCEILGCGATADAYHMTAPHP
                     EGLGAKNVMIAALRDAHMRPEDIDYINTHGTSTPLGDTAEIKAITD"
BASE COUNT         1259 a         1473 c         1129 g          935 t
ORIGIN      
        1 cctcatccgt gacctgaaga ccctcgaagc aaaaaaatac accctcttcc tcttcgccga
       61 gaacccgcgg caactggaac gcctccatag catcttccag gacctgaaag ccgagatccc
      121 gttcaccccc atcgcccatt ccatccatga gggattcatc gacgaagacc tgaaaatagc
      181 ctgcttcacc gaccaccaga tcttccagcg ttatcataaa tacaaggtca aacaggcgta
      241 taataagaac aaggccatca ccctccgcac cctgcgggaa ttgcagcccg gcgactacgt
      301 cacccatatc gaccatggcg taggcgtata cagcggcctg caaaagatcg aggccaacgg
      361 ccgcctgcag gaggcggtgc gtatcatcta taaggatagc gacatcctct atgtcaacat
      421 caactccctg cacaaaatat ccaaatacac cgggaaggaa gggacggtgc caaaggtcaa
      481 caagctcggc agcgacgcct ggcaaaaact aaaggaaaaa acaaagacaa aggtcaagga
      541 gatcgccttc gacctcataa aactctacgc ccagcgcaag gcggagatag gctttcagca
      601 cagcccggac aattacatgc agacggaact ggaagcctcc tttatctacg aggacacgcc
      661 cgaccaggga aaggctaccg ccgacgtcaa aaaagacatg gaagccccat cccccatgga
      721 tcgcctcgtc tgcggcgacg tcggcttcgg aaaaacagaa gtggcgataa gggccgcctt
      781 caagacctgc tgcgatggca aacaggccgc catcctcgtc cccacgacca tcctggcctt
      841 ccagcactat aagaccttta gcgatcgact caaggacttc cccgtcaaag tcgattttgt
      901 caatcggttc aaatccgcaa aagagaagaa agagaccctg aaacaactgg aagccggcca
      961 gaccgaaatc atcatcggca cccacgccct gctcggaaaa gaagtgaagt tcaagaacct
     1021 cggcctcctc gtcatcgacg aagaacaaaa gttcggcgtg gcccacaagg aaaagataaa
     1081 aacgctccgc acccacgttg actgcctcac cctgacggct acgcctatcc cccggacact
     1141 ccagttcagc ctgatgggcg cccgcgacct cagcatcatc aataccccgc cgccaaaccg
     1201 gcaacccatc cagaccgaat tgcacgggta taatgaggac ttcatccgcg acgccatcta
     1261 ttacgaaacg gaacgcggcg gacaggtgtt ctttatctac aaccgggtac agggactggc
     1321 cgaaatgtcc gccctcatcc agggcctctg ccccgatctc agcatcggct acgcccacgg
     1381 acaaatggaa ggcaacgatc tcgaaaaccg catcctcagc tttatcgatc ggaaatttga
     1441 tgtcctcatc tgcacaaata tcgtggagag cggcgtggat atccccaacg tcaataccat
     1501 catcgtcaac aacgcccatc atttcggcct tagcgacctc caccagctcc ggggtagggt
     1561 aggacgcggc aacaggaagg cattctgcta cctcctcgcc ccctccctgg ccaccctgcc
     1621 cgacgactcg cgcaaacggc tccaaaccct ggaacaacac agcgatctcg gcagtggctt
     1681 ccagatcgcc atgcgcgacc tcgatatccg cggcgccggc aacatgctcg gcggggaaca
     1741 aagcggcttc atggtcgaca tcggtttcga gacctatcag aagatcctcg acgaggcgat
     1801 aaaagaattg aaacgcaccg aattccgcga actcttcaag gaagagatct caaaacagga
     1861 agactatgtc caggactgca ccatcgacac cgacctggag atcctcatcc cagacagcta
     1921 cgtggaaagc atcaccgaaa gactgtccct ctatacccgc ctggacaact gcgacagcga
     1981 agatgagctg cagcagttcc acgctgaact gatcgaccgc tttggcccca tccccccaca
     2041 ggtggaagac ctgttcgata ccgtccgcat ccggaaactg gccgtaagtc tgggattcga
     2101 aaaactgatc ctcaaagagg acacgatgcg ctgctatttt atcgaccggc ccgattcacc
     2161 ctacttcgaa tccgaaacct tccacctcat cctcgaatac ctgcaaaaac agaccaacaa
     2221 ggcccggcta aaacaggccg gcaaacactt cctcctggtg gtcgacgacg tccggtcgat
     2281 ggccgatctc ctcggcttcc tccagcggat gagcaaattc gccctcgccc cccgggccct
     2341 catgggataa atgtttgacc ttacaagaca taaaaatttg caaaaaggat actatattta
     2401 aggagtaaag ccaaaaaact actaccatgc cggaagaata ccccctcacc acccccacca
     2461 gcgacgagag aacgctgggc atcctctccc atatcctggc gatatgcccc ggaataggca
     2521 tcttcggccc cctcgtgatc tggctgataa aaaaggatga atcgcaattc gtaagtgaga
     2581 atgcaaaaga atccctcaac tttcaactga ccatgatcat cgcctttatc atcagcggta
     2641 tcctgatggt cgtgctcatc ggcttcttcc tgttctggat cctgggcatc ctcaatatca
     2701 tcctggtgat catcgcaacg atcaaggcca gcgagaacaa gatctatcgc tatcccttca
     2761 acctgcggct gataaaatag aaagccttaa agcgccttct ccataatata atgtggaatg
     2821 gtaacctcga cgaactcatc ccccgtgatg ccatagccga gcttctgata aaagccgacc
     2881 gcggttttcc gcgcgtgcat acagagtttc ttgtaaccca ggtcacgggc gatattctcg
     2941 gcaaagatca tcagggcgcg cccgataccc ttaccctgca tattgttggg gacagccatc
     3001 tgccgcagac ggaccgtgat cgcgtccatc cgggaaagca tacaacaacc caatagcctg
     3061 tcatcttcga atgcacccat caggatatcg tccttctccc ggtccaactc ctccttggta
     3121 aagagtaacc ccagcggttt acgcaatatt tcattgcgca gattcaccat ctgctggtac
     3181 tccttggtcc catgatctat catcctaagc gccatgctgt tgttatattt tctatacacc
     3241 gggccggtga ttggcccagg ccccggtccg gggaggccga agttatttaa atttactaat
     3301 tccgggctgt aaatctgttt tttgttctgt tagtcggaaa ataatacttt tgcatccgta
     3361 taactaaaat ttaatcaaaa tgtcagacat cgctacaagg gttaagaaga ttattgttga
     3421 caagctcggg gttgaggaag ctgaagttac caatgaggct tctttcacca atgatctggg
     3481 tgcagactct ctggacaccg tcgaattgat catggaattc gagaaagagt tcaacatctc
     3541 cattccggac gaacaggccg agacgatcac taccgtcggt caggccatcg cttacctgga
     3601 agagcacgca aaataagcat tggctgcaca acgttgttct gggttcaccg caataaactc
     3661 gtttgatccg ccttttcaag cattttggct tagaaggcgg attttatcct taacataccg
     3721 gagccactgg gtggctcgtc cgaaggacag acattagccg ttttgctgga atcgggctaa
     3781 ttttctgcaa agcacgattc cagcacaaaa ttcctgggaa aagtccacta tgcaattgaa
     3841 aagagttgtt gtaacgggca ttggtgccct caccccacta ggtaacaata cgcaggatta
     3901 ctggaaggga ttgctaaatg gcgtttccgg tgctggttct attaccctgt tcgacgcggc
     3961 gaaattcaaa acccgcttcg cctgtgaact aaaaggcttc gaccccctga actacctcga
     4021 cagaaaagag gcccggaagg tcgaccgcta tacgcagatc gccctcgcag ccagcgacga
     4081 agcggtaaaa gatgccgcca tcgacaagga caccatgaac cccgaccgca tcggcgtcgt
     4141 tttcgccagc ggcatcggcg gcctgatcac cttccaggaa gaggtgatca attttgccaa
     4201 gggcgacggg accccccgct tcaacccttt cttcattccc aagatgatcc tggacatcgc
     4261 ggcaggccat atctcgatgc gacatggctt ccgcggcccc aacttcgccg tgacgagcgc
     4321 ctgcgcctcc tctaccaatg ccatcatgga agcctttaac ctcatccgcc tcgggatggc
     4381 cgacatcctg atcagcggcg gctccgaagc cgtcgtcagc gaggccgggg tagggggctt
     4441 caacgccatg aaagccctga gtgaacgcaa cgacgatccc gcaacagcca gccggcccta
     4501 cgacaaggac cgcgacggct tcgtcatggg agaggccgcc ggcgtactgg tgctcgaaga
     4561 actggaacac gccaaagccc gcggcgcccg gatctattgc gagatcctgg gttgcggcgc
     4621 caccgccgac gcctaccaca tgaccgcacc ccaccccgaa gggctgggcg ccaagaatgt
     4681 catgatcgcc gccctgcgcg acgcccatat gagaccggag gacatcgact atatcaacac
     4741 ccacggtacg tccacccctc tcggggacac cgcggagata aaggccatca ccgatg
//
LOCUS       DATGFG010000145        11894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_199748, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000145 DATGFG010000000
VERSION     DATGFG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199748"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VKQ52_02660"
     CDS             <1..357
                     /locus_tag="VKQ52_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86109.1"
                     /translation="LRTFPMGGGSFGAVKGEFPAFTLNGDGVFTAYIGFLANTDPGFT
                     TNILFQVFVYNPFDAPKIPQAVISITKSFSGGFLPVRADLSAFRNQPIKICVLVTSQP
                     PSRGAAAYWLAPTITY"
     gene            complement(383..709)
                     /locus_tag="VKQ52_02665"
     CDS             complement(383..709)
                     /locus_tag="VKQ52_02665"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ86110.1"
                     /translation="MTAQTLDVFQVIADPSRRQMLQLLSKDSMTINALAENFDMSRPA
                     VSKHIKIMYNAGFISIRDIGRERHCTLKQDGFNELQDFINYFDKFWASKLKKLETILN
                     NKAKTK"
     gene            complement(706..1116)
                     /locus_tag="VKQ52_02670"
     CDS             complement(706..1116)
                     /locus_tag="VKQ52_02670"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLZ86111.1"
                     /translation="MQEEIRQTWLFKQSPQEVWEFLTKPELLEQWLGKTDFQPIVGHK
                     FRFVSPYGNDSFCEVLEVTPFAKLSFSWQKRSAKGNTPFNSKVVWTLVPKENGTELQL
                     VHNGFTALEDLAGHEKGWTTCLKQLEELLNTITS"
     gene            complement(1160..1984)
                     /locus_tag="VKQ52_02675"
     CDS             complement(1160..1984)
                     /locus_tag="VKQ52_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HLZ86112.1"
                     /translation="MELFVFVNDKLVPASQASLLISDLAIQRGYGVFDFFKTLDHAPV
                     YLEEHLDRFFYSASRLRLEMGKSREELKGLIGELLAKNRIADSGVRLELTGGYSTDGY
                     TPARPNLVISQQPLTAPITAELPKSIRLITYPHTRQLPDIKTIDYLMAIWLQPLIREK
                     GADDVLYHQDGVLAECPRSNFFLVTAGGTLVTPARDILKGITRMKLLETAREKIKVEV
                     RDLRINELRTAKEAFITSTTKHVLPVTAIDGVAVGDGQVGPVARWLSEELYRLARG"
     gene            complement(1989..3116)
                     /locus_tag="VKQ52_02680"
     CDS             complement(1989..3116)
                     /locus_tag="VKQ52_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HLZ86113.1"
                     /translation="MRISFMVLLAFLFWIPAGAQNKRARELGLRIGVLEPGEWDAITD
                     VPGVKVGQTTLNRGDSVHTGVTAILPYDGNIFQQKVPAAIFVGNGFGKLAGSTQVMEL
                     GNLETPVVLTNTLGVATAIQAVVEYTLREKGNENVFSVNAVVGETNDGSLNDIRGMHV
                     RKEDVWSALTSAKTGPVEEGCVGAGTGTVCFGFKGGIGTASRKLPASLGGYTVGVLVQ
                     TNFGGVLEIDGAPVGKELGRFAFSDKLKNPVDGSCMMVVATDAPLDSRNLGRLAKRAF
                     MGLAKTGGIAANGSGDYVVAFSTNDSLRIPHQPTGSLLRRAELDNEAVSPLFMAAIEA
                     TEEAIINSLLAAKTTKGKTQTVEALPMETVLPILRKYHRLK"
     gene            complement(3141..4298)
                     /locus_tag="VKQ52_02685"
     CDS             complement(3141..4298)
                     /locus_tag="VKQ52_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ86114.1"
                     /translation="MRMILPAACLWALTGAAQSVTPAVDKRFVKHILTNEFVSEGVAI
                     ADVNRDGKPDIFAGAYWFEAPNWTRHALAPEKHYSPATEFSNSFLDYSMDVNQDGWPD
                     LVRISLPGEEAVWYENPGKKPGYWPMHPLLTNCGNESPAFVDVDGDGRPDLLCNDPVA
                     KEMIWLKSPVVKGDTVWKRYVIARGAGAPGTNRYTHGLGLIDMNRDGRKDVVITKGWW
                     ECPADPRTPDWVFHPAGLGEDCSQIYALDAKGDGALSLVSSSAHRYGIWWHEKVDTGW
                     VHHTLFSGVSETHALALADVNGDGHPDLVTGKRYFAHNGEDPGAYDPATLYWFEFHPG
                     ANPTWIPHLVDGDSGVGLQVLVQDLDGNGLPDIIVANKKGVFFFEQVYVGS"
     gene            4349..5185
                     /locus_tag="VKQ52_02690"
     CDS             4349..5185
                     /locus_tag="VKQ52_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ86115.1"
                     /translation="MLLRTACCLLLLLTGYTNHAQTIHYGDNNAAGHYLNTRGIRLYY
                     ETYGKGDPLLLLHMNGESMKVFYNQIPWFSQYYRVIAVDSCAHGKSVDASDSLSFEEM
                     ADDFNALCDSLHIDSCYVIGWSDGGISALLLALRHPEKIRKMVVSGPNLWPDTTGVVP
                     YIFHLMERTADSLHHKGSLTAAEKNLLKTTELDLYQPHITLEQLHAIKCPTMVVGGDH
                     DAIPSLHLWQMAQNIPQSYLWIVPNSGHSVVIYKKAQFNPLVNDFFRKPYRRIEGMDV
                     MK"
     gene            5309..5512
                     /locus_tag="VKQ52_02695"
     CDS             5309..5512
                     /locus_tag="VKQ52_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86116.1"
                     /translation="MDQSKENQKIKNPPSNEQQKKDNKKAGQQQKNKPNREANRPPDK
                     KEQDETDESLRENMGSGKRQDDN"
     gene            complement(5551..6015)
                     /locus_tag="VKQ52_02700"
     CDS             complement(5551..6015)
                     /locus_tag="VKQ52_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86117.1"
                     /translation="MNANKFLLFLLLLSGEAVLAQHGGDQSVPRSVQRNFQRDYPEAG
                     DARWSSSGKEWHADFTDHSRYDRGEMVAHYDQTGRHVDSHIPYDRNDVPNPVIHHVER
                     KYPGGSDYSYTRIERPGAQAVFQVGLSLNGRHTNKYVDDQGRERSYQDHHHY"
     gene            complement(6099..6953)
                     /locus_tag="VKQ52_02705"
     CDS             complement(6099..6953)
                     /locus_tag="VKQ52_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLZ86118.1"
                     /translation="MKPGGIVVVTSANAATARMLIPRLRERGWHVIGLLRHPADVGAD
                     ETIADWTHAAAAKEAFGRADVILHLSGDANARNRAAYRAANYLTTKLVAENVAREKQQ
                     RIIYLSYANASVTERNHYLHYKGAAEKLLLSTGKEVVIFRCPVILDAPGAVSRIDELF
                     IAKKGRAVPVIGDGRQKMRPVYRGDVVEAILAAMQRGRMGIYALSGPEEITVNELVRL
                     ANRDPRVRMRHIPGWLAFLLSRVVPGLSATFVDLLLRHSNSVYDPGAYREFGVKATPV
                     SRLYEQLG"
     gene            7052..7645
                     /locus_tag="VKQ52_02710"
     CDS             7052..7645
                     /locus_tag="VKQ52_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86119.1"
                     /translation="MKTFVKLPALILSLYPLFTGCSGTPSQARTTPPVAKKLHVKVDS
                     LTREPQVTNIVSGNPRSQITRVVSYSYLDVEDYEEDTFTRAPSFQMMPGYARFLWKYD
                     PHPKVVLTKKQIRRLMAIISVPKSYKKIQSTCYSPRNCFCFYNAKKEIIGYYQVCFEN
                     SRLYCVPEFNGSESGTLSESGAKRLRNFCLSAGITVR"
     gene            complement(7647..8414)
                     /locus_tag="VKQ52_02715"
     CDS             complement(7647..8414)
                     /locus_tag="VKQ52_02715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLZ86120.1"
                     /translation="MLTVCCFIVCCRLAAQPIEWPNHKRAAIVLTYDDALKSQLDTAV
                     PQLRRAGFKATFFLTSDIDSQSIPRWRAVAREGFELANHTLYHPCPNGEDNPVSSVEY
                     TPYKMVREIEVMNHFLFAVDGRSGRTYAYPCAETTAGGKDYVDTLRRYGVVKYARVGG
                     DTSAVITDLAHLDPLRVPSLGLEDSTSAEMIISFVKSVQQRGGMGIIMFHGIGGDYIT
                     TSSRVHQALLDYLVENRRSIWVTTFQQAMDFVVKRTR"
     gene            complement(8423..9502)
                     /locus_tag="VKQ52_02720"
     CDS             complement(8423..9502)
                     /locus_tag="VKQ52_02720"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HLZ86121.1"
                     /translation="MENRGLLFIPDISGFTRFVNETDIEHSRLIIQELLETLINTNSF
                     GLEISEIEGDAILFYKFGEVPKMEALFKQVEAMFLEFHKHLIAYDRSRFCQCKACRSV
                     VGLTLKIVTHYGEFTGYNVKTFNKLIGRDIIVAHQLLKNDIDQHEYWLVTDSLAPEKP
                     LSGPEPWMEWSSSVKQTDSGEIPFHYTQLGELKNELEPDPFPVSEFARRTKVLSVTRE
                     YDTDIITLFHATGDFSFRHRWQEGVRKVEEISHWLPRVGARSRFIMEDRETVIRSSSY
                     SYRPDHIEFSETDEGTQDLHYFTLEALDPARSRLTIDFYRNKSLLGGLLFDLTRKKKL
                     EDAYQRSLANIEGLLKELPSFAMEL"
     gene            9562..11148
                     /locus_tag="VKQ52_02725"
     CDS             9562..11148
                     /locus_tag="VKQ52_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HLZ86122.1"
                     /translation="MKDTFILYILLVFIILLLVMAAQKLRIAAPLLLVAGGLLLSWVP
                     ALSHIQIDPELIFLVFLPPLLYEAAWDISWKEMWRWRRVISVFAILIVILTSGVVALV
                     SWAWIPGFTLALGFLLGGIVSPPDAISATSVMRMVRVPKRLTHILEGESLLNDASSLV
                     IYRFALAAVATGRFVFHEALGSFFLVIIMGVATGILIGLAYYAIHRWLPTNPDIDIVL
                     ALTAPYVMYLTAESMHFSGVLSVVCGSLFLSTRNYQYLSHRSRLRGANVWTTIGFVLN
                     GLIFMLIGLELPEIIRQLGAVSLSQAIIYGTGITLLLIVARMLCTFSASAFTVFISRY
                     ITTADNRPGLKGPILMGWAGMRGVVSLAAALSIPYTLPGGAAFPQRNLILFITFVVIV
                     LTLLVQGLSLPALIRRVNMKDPDHDLTPEEQDLQVRRQLTEFGLDFLDENFPGRIDTN
                     PLLHQLRSRLCTDEEQLHRQSPETQEEYKHIYLKLLKKQRQYLQQLNRSIATDEEIIR
                     KYQSILDLEQEKLICLYEPR"
     gene            complement(11145..>11894)
                     /locus_tag="VKQ52_02730"
     CDS             complement(11145..>11894)
                     /locus_tag="VKQ52_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLZ86123.1"
                     /translation="YRQGASDPVLIAPETYTLDQHFLDRPGNEEIQLDLLKDYRNNVK
                     MYPLFHDYFRQYQPPLLAVWGNRDPYFLPAGAEAYKKDNPNATVKFYDTGHFALETHA
                     AEIGQDVLGFLQKVYPATAGPHPADVIVRESPFSVKETIDRLQDFLQQQGVTIYARID
                     QQKELDKVGLALAPLEYLLFGNPKAGGPVMQENPVAAIALPLKVIAWEDRDKKVWVAY
                     CSGDSVAGQFGISARAAAPLHLDGLIKKILG"
BASE COUNT         2684 a         3837 c         2751 g         2622 t
ORIGIN      
        1 ctgcgcactt ttccgatggg tggtggctcc tttggcgctg ttaagggcga gttcccggca
       61 tttacgctca atggagacgg cgtttttacg gcctatatag ggtttctggc caataccgac
      121 cctggcttta ccaccaatat cctgttccag gtatttgtct acaatccgtt cgatgcgcca
      181 aagattcccc aggcggtcat cagcattaca aaaagcttta gcggcggttt tctccccgtt
      241 cgagccgacc tctccgcctt tcggaaccag ccgataaaga tctgtgtgct cgtcaccagc
      301 cagcccccct ccaggggcgc ggcagcctat tggctcgccc cgacgataac ttactgatga
      361 cccggagccg tgttttgatc tttcattttg tctttgcctt gttgttcagg atggtttcca
      421 attttttcag tttgctcgcc cagaatttat caaagtagtt gatgaaatcc tgcaattcat
      481 taaaaccgtc ctgctttaag gtgcaatggc gttctctgcc gatgtctcga atagaaatga
      541 agccggcatt gtacatgatt tttatatgtt tggaaaccgc gggacggctc atgtcgaagt
      601 tttccgctaa agcgtttatc gtcatactgt cttttgaaag cagctgcaac atttgtcttc
      661 ggcttgggtc ggcaattacc tggaaaacgt caagcgtttg tgctgtcatg atgtgatagt
      721 gtttaatagt tcttcgagtt gctttaagca ggtggtccag cctttttcgt gaccggcaag
      781 atcttccaat gcagtgaaac cattatgtac taattgcaat tccgttccgt tctctttcgg
      841 aacaagcgtc cacacaactt ttgaattgaa aggggtattg cctttagccg atcttttttg
      901 ccacgaaaag gaaagtttgg caaatggcgt tacttccagc acttcgcaga aggaatcatt
      961 gccgtacgga ctgacgaaac ggaatttgtg tcccacgatc ggctgaaaat ctgttttccc
     1021 cagccattgt tccaatagtt caggtttggt tagaaattcc caaacttctt gtggggattg
     1081 cttgaaaagc cacgtttgcc taatttcttc ctgcatggta ggtaaaataa aagttactca
     1141 aatatacgta actttaaagt taccccctgg ctaaccggta caactcctca ctcaaccacc
     1201 tcgccaccgg ccccacctgt ccgtcgccaa ccgccacccc atcgattgcc gtcaccggca
     1261 ggacatgctt cgtcgtactc gtaataaagg cttctttcgc cgttcgcaac tcgttgatcc
     1321 tcaaatcccg cacctccacc tttatttttt ccctggccgt ctccaatagc ttcatccgtg
     1381 tgatgccttt caggatatcc cgcgccggcg tgacaagcgt ccccccggct gtgacaagaa
     1441 agaaattcga ccttgggcat tccgcgagca ccccgtcctg gtggtaaagc acatcatccg
     1501 cccccttctc ccgtatcagg ggctgcagcc agatcgccat caggtaatcg atcgtcttga
     1561 tatccggcaa ctgccgcgta tgcggatagg tgatcagccg gatactcttt ggcaactccg
     1621 cggtgatcgg cgccgtcaac ggttgttggc tgatcacgag attaggtcgt gccggtgtat
     1681 acccgtcggt cgaatatcct cccgtaagct ccagccgcac ccccgaatcc gcgatcctgt
     1741 tctttgccag caactcgccg atcaacccct tcaattcctc ccggctcttc cccatctcca
     1801 gccggaggcg ggaagcggaa taaaaaaaac ggtccagatg ttcctccagg taaaccggcg
     1861 catgatccag cgtcttgaaa aaatcgaaaa caccatatcc ccgctgtatg gccagatcgc
     1921 tgatcagcag cgaagcctgg ctggcgggaa caagcttgtc attgacaaaa acaaacagct
     1981 ccatattatt atttcaaccg gtgatatttc ctcaatatcg gcaatacagt ttccatcggc
     2041 agcgcttcta cggtctgtgt cttccccttc gtcgtctttg cggcaagaag cgaattgatg
     2101 atcgcctctt ccgtggcctc gatagccgcc atgaataaag gggataccgc ctcattatcc
     2161 aactcggccc ggcggagcag cgaacccgtc ggctgatgcg ggatgcgcag cgaatcattc
     2221 gtcgagaaag cgaccacata gtccccgctt ccgttggcgg cgattccccc tgtctttgca
     2281 agacccataa aggcccgctt cgcgagacgc ccgagattcc ggctatcgag gggcgcgtcg
     2341 gtggcaacca ccatcataca gctgccgtct acgggattct tcaatttgtc gctgaaggca
     2401 aaccgcccca gttccttacc cacgggtgcg ccatcgatct ccagcactcc gccaaagttc
     2461 gtttgcacga gcacacctac cgtatacccg ccaagcgatg ccggcagttt tcgggaagcg
     2521 gtgccgatac cccccttaaa gccaaaacat accgttcccg tccccgcgcc gacacaaccc
     2581 tcctccaccg gtcccgtctt cgccgaggtg agcgcactcc acacatcctc tttccggaca
     2641 tgcatccccc ggatatcgtt cagggagcca tcgttcgtct ctcccacgac ggcattcacg
     2701 gagaatacgt tctcgttccc tttctcccgc aaggtatact ccaccacggc ctggatggcg
     2761 gtggctacgc ccagggtatt ggtcagcact accggtgtct caagattgcc gagctccatc
     2821 acctgcgtcg acccggccaa tttgccaaac ccgttgccga caaagatcgc cgccggcact
     2881 ttctgctgga agatattccc gtcatagggg aggatggcgg ttactcctgt atgcaccgaa
     2941 tcgccccggt tcagggtcgt ctgccccacc ttgacacccg gtacatccgt aatggcgtcc
     3001 cattcgcccg gctccaacac cccgatccgc aaccccaatt cccgcgcccg cttattttgt
     3061 gctccggccg ggatccaaaa aaggaaagca agaagaacca taaacgatat tctcataaca
     3121 gccaaatttg tcgcaatgaa ctacgagccg acgtaaacct gctcaaaaaa gaacactccc
     3181 ttcttattgg caacgatgat atccggcaaa ccattgccgt cgaggtcctg caccagcacc
     3241 tgtaacccaa cccccgaatc accgtccacc aggtgcggta tccaggtcgg atttgccccg
     3301 ggatggaact cgaaccagta caatgttgcc ggatcatatg cacccgggtc ctccccgtta
     3361 tgcgcaaaat aacgcttccc cgtcactaga tcgggatggc cgtcgccatt gacatccgcc
     3421 agggccagcg catgcgtctc cgagaccccg gaaaagaggg tatgatgcac ccaccccgta
     3481 tcaaccttct catgccacca gatcccatac cggtgggcag aactgctgac aagactcagg
     3541 gctccgtcgc ccttcgcatc cagcgcatag atctgcgaac agtcctcgcc aagccccgcc
     3601 ggatgaaaca cccagtcggg cgttcgcgga tcggccggac attcccacca tcccttcgtg
     3661 atcaccacat cctttcgtcc atccctgttc atatcgataa gccccaggcc atgcgtatac
     3721 cggttggtcc ccggcgcccc cgctcccctg gcgatcacat aacgtttcca gaccgtatcc
     3781 cccttcacca ccggcgactt caaccagatc atctccttcg ccacaggatc gttgcaaagc
     3841 aggtccggcc ggccgtcacc atcgacatcc acaaaggcgg gcgactcatt cccgcagttg
     3901 gtcagcagcg gatgcatcgg ccaatacccc ggcttcttcc cgggattctc ataccacacg
     3961 gcctcctctc ccggcagact gatcctgacc aggtcgggcc aaccatcctg gttgacatcc
     4021 atgctataat ccaaaaagga attgctgaac tccgtagccg gtgaataatg tttttccggc
     4081 gcaagggcat gccgggtcca gttcggcgcc tcgaaccaat acgctcctgc aaaaatgtcc
     4141 ggcttcccgt cccggttcac atcggcgatg gccaccccct cggacacgaa ttcattggtc
     4201 aggatatgtt ttacaaatcg tttgtcgaca gccggcgtaa cgctctgcgc agccccggtc
     4261 agcgcccata gacaggcggc gggaaggatc attcgcataa ggaaaagtaa taaaaaagcg
     4321 ctaatattag tgttacaaaa atcccgccat gctactgcgc accgcctgct gcctgcttct
     4381 cctgcttaca gggtatacca accatgccca gacgatccac tacggcgaca ataacgccgc
     4441 aggccattac cttaacacgc gcggtatccg cctctactat gaaacgtatg ggaaaggcga
     4501 tccgctcctg ctgcttcaca tgaacggcga atccatgaag gtcttctaca accagatccc
     4561 ctggttctcc caatattacc gcgtcatcgc ggtcgacagc tgcgcccatg gaaaatcggt
     4621 ggatgcaagc gactcgctca gcttcgaaga gatggccgac gacttcaacg ccctctgcga
     4681 cagcctccac atcgatagct gctatgtcat cggctggagc gatggcggca tcagcgccct
     4741 gctcctggca ttgcgccacc cggaaaaaat aaggaagatg gtcgtctccg gccccaacct
     4801 ctggcctgac accaccggcg tggtgcccta tatctttcac cttatggaac gcacggcgga
     4861 cagcctccat cacaagggct ctctcaccgc cgcagaaaag aacctgctaa agacgaccga
     4921 gctcgacctc taccagccac acatcaccct cgaacagctg cacgccataa aatgtccgac
     4981 catggtcgtc ggcggcgatc acgatgccat cccctccctc cacctctggc agatggccca
     5041 aaacatcccc cagtcctacc tctggatcgt tcccaactcc gggcactccg tcgtcatcta
     5101 caaaaaggcc cagttcaatc cgctcgtaaa cgatttcttc cgcaaaccct accgcaggat
     5161 cgaaggtatg gatgttatga agtgactcgc cgcgccatcc gccccaaatc ttccccaccg
     5221 gcaacggcca cattcgtctt gcccattcat aatcaattcg atactacaca ggcaggagaa
     5281 ttgcgtcgct actcaaaacg ctccgatcat ggaccagtct aaagaaaacc aaaaaataaa
     5341 aaacccgcct tccaacgaac aacaaaagaa ggacaataag aaggccggcc agcaacaaaa
     5401 aaataaaccg aacagggaag ccaatcgtcc gccggataag aaagagcagg acgaaaccga
     5461 cgaaagcctc cgtgagaaca tgggcagcgg caaacgccag gacgacaatt agccgcctat
     5521 ccccatccca tccggtcaat ccgcgtccgg tcaataatga tgatgatcct ggtagcttct
     5581 ttctcttccc tgatcatcta catatttgtt ggtgtgtctc ccattcagac tcagacccac
     5641 ctggaatacc gcctgtgcgc caggcctttc gatccgggta taggaatagt cgctgccacc
     5701 gggatacttc ctctctacat gatggatcac ggggttggga acatcgttcc ggtcatacgg
     5761 gatatgggaa tcgacatgac ggccggtctg gtcgtagtgg gcaaccattt ccccacggtc
     5821 gtagcggcta tgatccgtaa aatcggcatg ccattctttg ccggaggaac tccagcgagc
     5881 gtccccggct tccggataat cccgctgaaa attacgttgt accgatcgcg gaaccgactg
     5941 atcgcccccg tgctgtgcca ggacagcctc accgctcagc agcagaagga aaagaaggaa
     6001 tttgttcgca ttcataatag tagattttaa gtgtaagagt agttgcatga gtaggagaga
     6061 gaaatccatg ccagcatcca aaccgatcct ccaactaatc atcccaactg ctcatacaac
     6121 ctgcttacag gcgtagcctt caccccaaac tcccggtacg caccgggatc gtaaacgcta
     6181 ttgctatgcc ggagcaacag atccacaaag gtggccgaaa gcccgggaac cacccggctc
     6241 aacaaaaaag ccagccatcc ggggatatgt cgcatccgca cccgcggatc ccggtttgcc
     6301 agccggacaa gctcgttgac ggtgatctcc tccggtccgg acaaggcata gatacccatc
     6361 ctcccccgtt gcatcgcggc gaggatcgcc tccaccacat ccccccggta caccgggcgc
     6421 atcttctgcc ggccatcgcc gatcaccggc accgcccgcc ctttcttcgc gataaaaagc
     6481 tcatcgatcc gcgaaaccgc ccccggtgca tccaggatca ccgggcaccg gaagatgacc
     6541 acctccttac ccgtcgataa caataatttt tccgccgctc ccttatagtg caggtaatga
     6601 ttcctctccg taacactcgc attggcataa ctcaggtaaa tgatccgctg ttgcttttcc
     6661 cgcgcaacat tttcggccac cagcttcgtg gtaaggtaat tggcggcgcg gtaagcggcc
     6721 ctgttcctcg cattggcatc gcccgacaaa tgaaggatga catccgcgcg gccaaacgcc
     6781 tcctttgccg ccgccgcatg tgtccaatcg gcgatggtct catctgcccc gacatccgcc
     6841 gggtgccgca atagcccgat gacatgccat cccctctccc gcaaccgggg gatcagcatt
     6901 cgcgccgtcg cagcattggc actggtaacg acaacaatac cacctggctt cataatggga
     6961 ttttaaacaa agctaactag taattttttt taggttgcat agcattttgc gaaaaaaatt
     7021 cgttattgga attgccaaag tcttcctccg catgaaaacc ttcgtaaaac tacctgccct
     7081 catcctatcc ctatacccct tatttacagg gtgttccggc accccgtccc aggccaggac
     7141 taccccgccg gtggccaaaa aactacatgt aaaagtcgat agcctcaccc gcgaacccca
     7201 ggtcaccaat atcgtcagcg gtaatccccg ctcacagatc acccgggtgg tcagctattc
     7261 ctatctcgac gtcgaagact acgaagaaga taccttcacc cgcgccccct ctttccagat
     7321 gatgccgggc tatgcccgct ttctctggaa atatgatcca cacccaaaag tggtgctgac
     7381 aaaaaagcag atccgaagac tgatggcgat catcagtgtc ccaaagtcgt ataagaagat
     7441 acaatcgact tgctactcgc cgcgcaattg cttctgcttc tataatgcaa aaaaggagat
     7501 catcggctat taccaggtct gcttcgagaa cagcagactc tattgcgttc cggaattcaa
     7561 tggaagcgaa tccggcactc ttagcgaaag cggcgcaaag cgcttgcgga atttctgcct
     7621 ctcggccggc atcaccgtgc gatgacctac cgcgttctct ttaccacaaa atccatcgcc
     7681 tgctgaaagg tcgtgaccca gatcgatcgc ctgttctcga caagataatc cagcagggcc
     7741 tggtgcaccc gggaagaagt agtgatgtag tccccgccga tgccatggaa cataatgatc
     7801 cccatgcctc cccgttgctg cacgcttttt acgaaggaga tgatcatctc ggcgctggtg
     7861 ctgtcctcca gcccgagcga cggcacccgc aacggatcga gatgcgcaag atcggtgatc
     7921 accgcactcg tatccccgcc gacccgcgca tatttgacca ccccataacg gcgtaacgtg
     7981 tccacataat ccttgccgcc ggcggtcgtc tccgcacagg gataggcata ggtgcgacca
     8041 ctcctgccat ccaccgcgaa caggaaatga ttcatcacct cgatctcccg caccattttg
     8101 taaggagtgt actccaccga cgacacgggg ttatcctcgc cgttgggaca ggggtgatac
     8161 aaggtgtgat tcgccagttc gaaaccttcg cgggccaccg cccgccaccg ggggatggac
     8221 tggctatcga tatcgctggt caaaaaaaag gtcgccttga aacccgcccg ccggagctgc
     8281 ggaacggccg tatccaactg cgacttcaac gcatcgtcat aggtcaatac gatggccgcc
     8341 ctcttatggt tcggccattc gatcggctgt gccgccagcc ggcagcagac gataaaacaa
     8401 cataccgtta gcattctctt tatcataact ccatcgcaaa actcggtaat tccttcaaca
     8461 acccctcgat attggccaaa ctccgctgat aagcgtcctc cagcttcttc ttcctggtga
     8521 gatcgaacag caggccccct aacaggctct tgttccggta aaaatcgatg gtgagccggg
     8581 accgggccgg atccagcgcc tcgagcgtaa aataatgcag gtcctgcgtc ccttcatccg
     8641 tctcgctgaa ttcgatatga tccgggcgat aggagtagct gctgcttcgg ataacagtct
     8701 ccctgtcctc catgataaac cggctgcgag cccccacccg cggcagccaa tgactgatct
     8761 cctccacctt cctgaccccc tcctgccagc gatgccggaa actaaaatcg ccggtggcat
     8821 gaaacaaggt gatgatatcc gtatcatatt cccgggtgac ggacaacacc tttgtccgtc
     8881 gcgcaaactc ggaaaccggg aacgggtccg gctcgagctc attcttcaat tcgccaagct
     8941 gcgtataatg gaacgggatc tcccccgaat ccgtctgctt gacactgctg ctccattcca
     9001 tccatggctc cggaccgctt aacggtttct ccggggcaag actatccgtc accagccagt
     9061 attcgtgctg gtcgatatca ttcttcagca attggtgcgc cacgatgata tccctgccga
     9121 taagcttgtt gaacgttttg acattataac cggtgaactc cccgtagtgc gtgacgatct
     9181 tcaatgtcag cccgaccacc gaccggcagg ccttgcactg gcagaaccgg cttcggtcat
     9241 aggcgatcag gtgtttgtga aactccagga acatcgcctc cacctgcttg aacagggcct
     9301 ccattttcgg gacttcgccg aacttgtaaa ataaaatagc atctccttcg atctccgaga
     9361 tctcgagtcc aaagctattc gtgttgatca gcgtctccag cagctcctgg atgataaggc
     9421 ggctgtgttc gatatccgtc tcgttcacga atcgggtgaa tccgctgata tccgggataa
     9481 aaagcaaccc cctgttttcc attttttttc tttaaagtta ctatatcttt agaaagtgcc
     9541 ggcggcgaaa acctcccgaa tatgaaagac acatttatcc tttacatcct cctcgtcttt
     9601 atcatccttc tgctcgtgat ggcggcccag aaactcagga tcgccgcccc actgctactg
     9661 gtagcgggtg gcctcctgct tagctgggtc cctgcgttat cccatatcca gatcgacccg
     9721 gaactgatct tcctggtctt tctcccaccc ctcctctatg aagccgcatg ggacatctcc
     9781 tggaaggaaa tgtggcgctg gcgacgcgtc atcagcgtct tcgccattct catcgtgatc
     9841 ctcacctccg gcgtcgtcgc cctggtatcc tgggcatgga tccccggttt cacccttgcg
     9901 ctcggattcc tgctgggggg catcgtatcc ccacccgacg ccatctccgc cacctccgtc
     9961 atgcggatgg tccgcgtacc caaacggctg acccatatcc tcgaaggcga aagcctgtta
    10021 aacgatgcca gcagcctcgt tatctaccgc tttgcactgg ccgcggttgc caccggccgc
    10081 ttcgtcttcc acgaagccct tggcagcttc ttcctcgtca tcatcatggg cgtggcgaca
    10141 ggcatcctca tcggccttgc ctactatgcc atccaccgct ggctcccgac aaaccccgat
    10201 atcgacatcg tccttgcact caccgctccc tacgtcatgt acctcacggc ggaatccatg
    10261 cacttttccg gcgtcctatc cgtggtctgc ggctccctct tcctctcgac ccgcaactac
    10321 cagtacctca gccaccgcag ccgcctccgt ggcgccaacg tctggacaac catcggcttc
    10381 gtcctcaacg gcctcatctt tatgctcatc gggctcgaac tgccggagat catccggcaa
    10441 ctgggcgccg tcagcctttc ccaggccatc atctatggca cgggcatcac cctgctcctg
    10501 atcgtcgccc gcatgctctg caccttcagc gcttcggcat tcactgtatt catcagccgt
    10561 tatatcacca ccgccgacaa ccggcccggt ctgaaaggcc cgattctcat gggctgggca
    10621 ggcatgcgcg gcgtcgtctc cctggccgcg gccctctcca tcccctacac tctccccggc
    10681 ggagccgcct ttccccagcg caacctcatc ctcttcatca ccttcgtggt catcgtcctc
    10741 accctgctgg tgcaggggct aagccttccg gcactgatcc gccgggtgaa tatgaaagac
    10801 ccggatcacg acctcacccc tgaagaacag gacctgcagg tgcgccggca actcaccgaa
    10861 ttcggcctcg acttcctcga cgagaatttc ccgggcagga tcgacaccaa ccccctcctg
    10921 caccaactgc gcagccggct ctgcaccgac gaagagcaac ttcatcgcca aagccccgaa
    10981 acccaggaag aatacaaaca catctacctt aaactcctga aaaaacaacg gcagtacctc
    11041 caacaactca accgctcgat cgccacagat gaagagatta tcaggaaata ccagtcgatc
    11101 ctcgacctcg agcaggaaaa actcatctgc ctctacgaac cccgttaacc aagtatcttt
    11161 ttgatcagtc catcgagatg caaaggggcc gccgccctgg cggagatgcc aaactgtccg
    11221 gcaacggaat ctcccgaaca ataggcaacc cataccttct tatcccggtc ctcccatgcg
    11281 atcaccttca gcggtaacgc gatcgctgcc accgggttct cctgcatcac cgggccaccc
    11341 gccttcgggt tcccaaaaag caggtattcc agcggcgcca gcgccagacc caccttgtcc
    11401 agttccttct gctggtcgat ccgcgcatag atggtgactc cctgctgctg caaaaagtcc
    11461 tgtagccggt cgatcgtctc ctttacggaa aaaggactct ccctgacgat cacatcggcc
    11521 gggtggggac cagccgtcgc gggatacacc ttttgcaaaa aaccgagcac gtcctgcccg
    11581 atctccgccg catgggtctc taacgcaaaa tgccccgtat catagaattt caccgtggca
    11641 ttcggattat cctttttata tgcctccgcc ccggccggga gaaaataagg atcccggttc
    11701 ccccagacgg ccagcagcgg cggctggtat tgccggaaat aatcatgaaa aagcgggtac
    11761 atcttcacgt tattccggta atccttcagc aggtcaagct ggatctcctc gttccccggc
    11821 cggtcgagaa aatgctggtc cagtgtatag gtctccgggg cgatcagcac cggatcactc
    11881 gctccctgcc ggta
//
LOCUS       DATGFG010000146         7243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_656265, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000146 DATGFG010000000
VERSION     DATGFG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656265"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..630
                     /locus_tag="VKQ52_02735"
     CDS             193..630
                     /locus_tag="VKQ52_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86124.1"
                     /translation="MEKAMGMKSISFRADEDLASQLEELYESKGGGAERIIPGFMTIR
                     KYTLHELQGVFTKEEFAALVAVQNGLLLDPKVQANGKMLNAHLEFSETYDRNISGNRA
                     ILNELLEKINNLTAAQVYFLQDEIEIFWRTGKETLEKFISRFA"
     gene            complement(743..824)
                     /locus_tag="VKQ52_02740"
     tRNA            complement(743..824)
                     /locus_tag="VKQ52_02740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            1160..3400
                     /locus_tag="VKQ52_02745"
     CDS             1160..3400
                     /locus_tag="VKQ52_02745"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HLZ86125.1"
                     /translation="MDTVAAIPKYNLTEDQEKKLILREYRALLRSLKPKLKAGDKELL
                     RIAFEMAAEAHKTMRRKSGEPYILHPLAVARICSEEIGLGVRSTICALLHDTVEDTDI
                     TLEDIEREFSGEIARIVDGLTKISNVVDVNASRQAENFKKILLTLTDDPRVILIKLAD
                     RLHNMRTLDSMKREKQLKIASETVYVYAPLAHRMGLYTIKTELEDLAMKYLEPEEYRE
                     IARKLSETKRERSRYINEFIKPLKDKLDKAGGFDFDIYGRPKSIHSIWNKMRKKGVAF
                     EEVYDLFAIRVILNSPPEKEKEDCWKVYSMITDEYNPSPERLRDWLSNPKSNGYEALH
                     TTVMGPQGKWVEVQIRSKRMNEIAEKGLAAHWKYKEGAADESRFDKWFQQIREVLNTQ
                     DNDSIDFLQDFKTSFLAEEIYVYTPKGDVKMLPVSSTALDFAFSIHSAIGVQCIGAKV
                     NHKLVPISHKLRSGDQVEIITSSKQKPNEDWLAFVVTAKAKSKIKDALKEEKRKIADE
                     GKYIVQRRLENFGAAYNQHNIDILTVFYKLNSSLDFFYQVSVRNIDLKELKEFQVLGD
                     RLEPPKPAKPAEVRPADGAHAVPRKDTELIIFGESSDRIVYTLANCCKPIPGDDVFGF
                     VSVGKGLTIHRTNCPNAAKLMANYGHRVVKTKWAKNKEISFLTGIRIVGMDDVGVINK
                     ITNLISGELKLNIAALTIEAKDGLFEGNIRIYVHDKEELDELVLRLKQLNGINTVVRY
                     DTETAS"
     gene            3577..4842
                     /locus_tag="VKQ52_02750"
     CDS             3577..4842
                     /locus_tag="VKQ52_02750"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HLZ86126.1"
                     /translation="MVDVILGLQWGDEGKGKIVDYFAPRYDLIARFQGGPNAGHTLYV
                     NDKKIVLHQIPSGIFHEKTLNLIGNGVVLDPVTLKRECESVAAFGVDYRKNLYIAQRT
                     HLILPTHRALDKASEMSKGQEKIGSTLKGIGPAYMDKTGRNGLRVGDLLDKSFTTQYI
                     KLRLKHQKLLDSLNFHEDLSEWEEEFFEALEFMRQFKIVNGEYFINDKLSQGKKVLAE
                     GAQGSMLDVDFGTFPFVTSSNTISAGVCTGLGVAPQQIRDVIGVSKAYCTRVGGGPFP
                     TELEDATGEELRRVGNEFGATTGRPRRCGWIDGVALKFACMINGVTKIVMTKADVLDA
                     FGELKICTAYQVNGETTNEIPFQMNKMKIEPQLQTFAGWKTDTTAITKYDKLPEKMRE
                     YVQFINKYLGVRIAYISNGPGRDQLIENL"
     gene            4935..5396
                     /locus_tag="VKQ52_02755"
     CDS             4935..5396
                     /locus_tag="VKQ52_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86127.1"
                     /translation="MAPKSDKEGKGSKKSAPQPKETPEKPSSKAKKPADEDDDDDDLD
                     NDDDAPKAASKKPASKAASKSKKEDDDDDDDEGEDYDEVDEWEKVEEEEEWDPDFDEF
                     DIPKSKTKKSTGGNTSKKTTKGDEDDLGLDEDFKDLDLFNDSGFEEEEDDF"
     gene            5496..6719
                     /locus_tag="VKQ52_02760"
     CDS             5496..6719
                     /locus_tag="VKQ52_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HLZ86128.1"
                     /translation="MDSHSYESSPPGFECLLAAGAIDTFTATAGDAFSGLQKWAAGRE
                     DWLFGHFAYDLTAETEGVTGPAADPIGFPDLFFFIPIHLIRLEKNSITIECLEADPET
                     IWWQISEAAPAEPVTPAPAKPVTPAPAPPIAFTPRFTRDEYLSTVDALRQHILRGDCY
                     EINFCQEFFAQPVTIDPYATWCALSQASPNPFAAFYRVGERYLLCASPERYLKKTGDT
                     LFSQPIKGTAPRQQQDPAADDRQREALYHSGKDRAENVMVVDLVRNDLARICIPGSVR
                     VAELYGIYPFPQVFQMISTVTGEMLPGLDWTDAIRASFPMGSMTGAPKHSVARLIARY
                     ERSRRGLFSGAVGYVTPNGDFDFNVVIRSLLYNRESRYLSYQVGSGITFYSDPAAEYE
                     ECLVKAEGIRKALGH"
     gene            complement(6766..>7243)
                     /locus_tag="VKQ52_02765"
     CDS             complement(6766..>7243)
                     /locus_tag="VKQ52_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86129.1"
                     /translation="RSGSRSFWDLERYLPAESRAHVKKFIGTQYMFEGQGSVTTLTRQ
                     EATEQLSGSAMYVFHRDLTAKELTTARTIAVTGKYHSSVIAKYTLLDSASFARYNPDF
                     DKIMASSNNSYDLKLPADKMDLFIANKYDILNESAQLLAGQDTIENAAGRVVAINK"
BASE COUNT         1845 a         1928 c         1943 g         1527 t
ORIGIN      
        1 attggtacaa gatagcttga atatcggttt caggtgcgcg gaccggcggc atgcgtcgcc
       61 ggccttaccc tctcatccgg tttacgacgt caggcgatca caaataatta atcttgaaaa
      121 aatatttaaa aaatcttgaa aatatatttg ttaattattt aaaatttcct tatcttttgc
      181 taattaattt ttatggaaaa agctatggga atgaaaagta tcagctttcg cgctgatgaa
      241 gacctcgcat cgcaattgga agaattgtat gaaagtaagg gtggaggggc cgagaggatc
      301 atcccgggct ttatgaccat tcggaaatat accttacatg aacttcaggg ggtttttaca
      361 aaagaggaat ttgcggcgct tgttgccgtt caaaacggat tgcttctcga tcccaaagtc
      421 caggcaaatg ggaaaatgtt aaatgcccac ctggaatttt cggaaacata tgatcgcaat
      481 atttccggca accgcgcgat attgaacgag ttacttgaaa aaatcaataa cttaacggcg
      541 gcgcaagttt atttcctcca ggatgaaatt gaaattttct ggcgcaccgg aaaggagacc
      601 ttggaaaaat tcataagcag atttgcttag gccaaggact acaacttcat attagttctt
      661 tgaaatactc cctctgcaaa agttgccagg aaatgggaat ggccaaaaaa taaaaggctc
      721 accgaagtga accttttaaa agtgcgggcg ataggggtcg aacctacatg gattgctccg
      781 gcagatccta agtctgctgc gtctgccaat ttcgccacgc ccgcattacc aattaagtct
      841 cttcaagcgt cttccagcaa attcccagca aatttggata aacagccgaa gatcgccaaa
      901 tatcaatttt ctatcgcctt agttctcaac gtccagcgac gaccgacgga aacggccaat
      961 tcaaaaccga ccagatccta agtctagtgc gtctgccaat ttcgccacgc ccgcgacaga
     1021 attaccggaa atgccctttg tgaaaaaccg gatatttcct gcaaagatcg gttgtccgaa
     1081 gcctcctccc ggggagccgg gtccttttgg agaaggcgtg caaaactagg tctttttttt
     1141 gtaatttgtg cgtatagata tggatactgt cgctgctata ccgaaataca atcttacgga
     1201 agaccaggag aaaaagctta ttctacgcga gtaccgtgcc ctccttcgtt cgttgaagcc
     1261 caagctgaaa gcgggcgata aggagctgct ccgtatcgcc tttgaaatgg ccgccgaggc
     1321 gcacaagacg atgcgccgca aaagcggcga gccctatatt ctgcatcccc tggctgtggc
     1381 caggatctgt tcggaagaga tcgggcttgg ggtccggtcg accatctgcg ccctcctgca
     1441 cgatacggtg gaagatacgg atatcaccct cgaggatatc gaaagggagt tcagcggcga
     1501 gatcgcccgc atcgtggatg ggttgacaaa gatctccaat gtggtggacg tcaacgcctc
     1561 ccgccaggcg gagaatttca agaagatcct gctgacgctg acggatgacc ccagggtcat
     1621 attgatcaaa ctggccgacc ggctgcacaa catgcggacc ctggacagta tgaagcgcga
     1681 gaagcagctg aagatagcct ccgaaaccgt ttatgtatat gcgccgcttg cccaccggat
     1741 gggtctttat acgatcaaga ccgaactgga ggacctggcg atgaaatacc tcgaacccga
     1801 ggaataccgg gagatagccc gcaaactgtc ggagacgaag cgggaacggt cgcggtatat
     1861 caacgagttt atcaagcccc tgaaggacaa gctggacaag gcgggcggct ttgatttcga
     1921 tatctatggc cggcccaaga gcatccactc catctggaac aagatgcgga agaagggcgt
     1981 cgcgttcgaa gaagtatatg atctttttgc catccgggtc atcctcaact ctccgccgga
     2041 aaaggaaaag gaggactgct ggaaggtgta ttccatgatc accgacgaat acaacccctc
     2101 acccgaacgg ctgcgggact ggcttagcaa tcccaaatcc aatggttacg aggccctgca
     2161 taccacggtg atgggtccgc aggggaaatg ggtggaggtg cagatccgct ccaaaaggat
     2221 gaacgagatc gccgaaaagg gcctcgccgc ccactggaaa tacaaggagg gcgccgccga
     2281 cgaaagccgc ttcgacaaat ggttccagca gatcagggag gtgctcaata cccaggataa
     2341 cgactctatc gatttcctgc aggacttcaa gaccagcttt cttgccgaag agatctatgt
     2401 ctatacgccg aagggcgatg tcaagatgct gccggtgagc tcgacggccc ttgactttgc
     2461 gttctcgatc cacagcgcca tcggggtgca atgcatcggt gcaaaagtga accataaatt
     2521 ggttcccatc agccacaagc tgcgcagcgg cgaccaggtg gagatcatca cctccagcaa
     2581 gcaaaagcca aatgaggact ggcttgcctt cgtcgtcacg gccaaggcga agagcaagat
     2641 caaggatgcc ctcaaggagg aaaaaagaaa gatcgccgac gaggggaaat atatcgttca
     2701 gcggcggctc gagaatttcg gcgccgccta caaccagcat aatatcgata tcctgacggt
     2761 cttctataaa ttgaactcct ccctcgattt cttttaccag gtttcggtca ggaatatcga
     2821 tctgaaggag ctgaaggaat tccaggtgct gggcgaccgg ctcgaaccgc ccaaaccggc
     2881 caaaccggca gaggttcgcc cggcggatgg cgcgcatgcg gttccccgca aggataccga
     2941 actcatcatc ttcggcgaga gcagtgaccg gatcgtctat accctggcca attgctgcaa
     3001 gccgatcccg ggggacgatg tctttggctt cgtctccgtc ggcaagggcc ttaccatcca
     3061 ccggaccaac tgccccaatg ccgccaagct catggccaat tatggacatc gggtcgtgaa
     3121 gaccaaatgg gccaagaaca aggagatatc cttcctcacc ggtatccgga tcgtggggat
     3181 ggacgatgtg ggagttatca acaagatcac caatctcatt tccggagagc tgaaactgaa
     3241 tatcgccgcg cttaccatcg aggcgaagga cgggcttttc gagggaaata tccgcatcta
     3301 tgtacatgat aaggaggaac tcgacgaact ggtcttgcgg ctaaaacagc tcaacggcat
     3361 caataccgtg gtgcgatatg ataccgagac ggcaagttag gttctgccgg gagttaccgg
     3421 aattcgcgca attttttttt aaagccggac ttcacctatt tttgccgctt caaagaaaag
     3481 gcgggccttt tggccgaagg ccgacgatta gccctgttgt gcggcggggc tggttttggg
     3541 gaaagacacg ccgcgcaact aaaatcactc aagtaaatgg ttgatgtaat cttaggcctc
     3601 cagtgggggg atgaaggaaa aggaaagata gtggattatt ttgctccccg gtatgacctg
     3661 atagcccggt tccagggcgg tcctaatgcg ggtcatactc tctacgtaaa cgacaagaag
     3721 atcgttctgc atcaaatacc ctccggcatc ttccatgaaa agaccctgaa cctcatcggc
     3781 aacggcgttg ttctggaccc cgtcaccctc aagcgggaat gcgagtcggt ggctgctttc
     3841 ggcgtcgact accggaagaa cctctacatc gcccaacgga cccatctcat tctgccgacc
     3901 caccgtgccc tcgacaaggc ttcagaaatg tcaaagggac aggaaaagat cggcagcacc
     3961 ctgaagggca tcggccctgc ctatatggac aagacgggaa gaaacggcct cagggtaggg
     4021 gatttgctgg acaaaagctt taccacgcaa tatataaagc tcaggctcaa acatcagaaa
     4081 ctgctcgaca gtctcaactt ccacgaggat ctctcggaat gggaagaaga gttcttcgaa
     4141 gcgcttgagt tcatgcggca gttcaagatc gtcaacggcg aatattttat caacgataag
     4201 ctctcgcagg gtaagaaggt cctggcggaa ggcgcccagg gcagcatgct cgacgtcgat
     4261 ttcggcactt tcccctttgt cacctcttcc aataccatct ccgcgggtgt ctgtaccggc
     4321 ctgggtgtag ccccccagca gatccgcgat gtcattgggg tcagcaaggc ctattgcacc
     4381 cgggtcggcg gcggtccctt ccccacggag ctggaagacg ctacgggcga ggaattgcgc
     4441 agggtgggca acgagtttgg cgctaccacg gggcggccgc gccgttgcgg gtggatcgac
     4501 ggtgtcgcgc tgaaattcgc ctgcatgatc aatggggtga ccaaaattgt catgacaaag
     4561 gcggatgtcc tggacgcttt cggcgaattg aaaatatgca ccgcctacca ggtgaacggc
     4621 gaaacgacca acgagatccc cttccagatg aacaagatga agatcgagcc acagttacag
     4681 acctttgcag ggtggaaaac ggataccacc gccatcacca aatatgataa actgcccgaa
     4741 aagatgcgtg aatacgtgca attcattaac aaatacctgg gagtcaggat cgcgtatatt
     4801 tccaacggtc cgggccgtga tcaactcata gaaaacctgt aactttcgtc agggaaacgg
     4861 tctaaaaatt aattttaaaa aaatttggcg gattaaaaac ttcgccgaaa atttacataa
     4921 caatcttatt ggacatggca ccaaaatctg ataaggaggg aaagggttca aagaaatccg
     4981 cgcctcaacc aaaggaaact ccggagaaac catcatcgaa agccaagaag ccggcggacg
     5041 aggatgatga tgatgacgat ctggacaacg atgatgatgc gccaaaggcc gcgtcaaaaa
     5101 aacctgcatc caaagccgcg tcgaaatcca agaaggagga cgatgacgat gatgatgacg
     5161 agggagagga ctatgacgaa gtggatgaat gggaaaaggt agaggaagaa gaggaatggg
     5221 accctgattt cgacgagttc gatatcccca aatccaaaac gaaaaaaagc accggtggca
     5281 acacctccaa gaaaacgacc aagggggatg aggatgacct cggccttgat gaagatttca
     5341 aggacctcga ccttttcaac gacagcggat tcgaggaaga agaagatgat ttttaagaag
     5401 tgaaaaggac ccacctttca tttcccatca ccgatcatga acgtataaag cgacaaatgt
     5461 tgacatgggc cttcccattc aacatttgtt gttttttgga tagccactcc tacgaatcct
     5521 cccccccggg ttttgaatgc ctgcttgccg ccggggccat cgacaccttt accgccacag
     5581 ccggcgatgc tttttccggc ctgcaaaaat gggccgccgg ccgggaggac tggctctttg
     5641 ggcattttgc ctatgacctc actgccgaaa cggagggcgt caccggtccc gccgccgacc
     5701 cgatcggttt tcccgacctc ttctttttca tccccatcca tctcatccgg ctggaaaaga
     5761 acagcatcac catcgaatgc ctggaggctg atcccgagac catctggtgg cagatcagcg
     5821 aggcggcgcc cgccgaaccg gtaacgccgg caccggccaa accggtaacg ccggcaccgg
     5881 cccctcccat cgcctttacg ccccggttta cccgggatga atatctttcc acggtcgacg
     5941 cgctacggca gcatatcctg cgcggggact gctacgagat caatttctgc caggagttct
     6001 tcgcacagcc ggtgacgatc gatccctatg ccacctggtg cgccctgagc caggcttcgc
     6061 ccaacccctt tgccgccttt taccgggtgg gtgagcggta tcttctttgc gccagccccg
     6121 aacggtattt gaaaaagacc ggggacaccc ttttctccca gccgataaag ggcacggccc
     6181 cgcgccaaca acaggatccc gccgccgacg accgccagcg cgaggcgctc tatcacagcg
     6241 ggaaggatcg tgccgagaac gtgatggtgg tcgatcttgt ccgcaatgac ctggcccgga
     6301 tctgcatccc cggctccgtc cgcgtggcag agctctatgg catctatccc ttcccccagg
     6361 tgtttcaaat gatctcgacg gtcacgggtg aaatgcttcc cggtctggac tggaccgacg
     6421 ccatccgcgc cagctttccc atggggtcga tgacgggggc gccaaagcac agtgtcgccc
     6481 ggctcatcgc ccggtacgag cgctcccgcc gcggactctt ttccggggcg gtcggttatg
     6541 tcacccccaa cggcgacttc gacttcaatg tcgtcatccg cagcctgctc tacaaccggg
     6601 agagccgcta cctgtcctac caggtgggat cggggatcac cttttacagc gaccccgcgg
     6661 ccgaatacga ggaatgcctg gtcaaggcgg agggcatccg caaggcattg gggcattaaa
     6721 aaaggggccc ggaggccccc tgtcggtatc taagtatcaa aaaaactact tgttgatggc
     6781 tactacgcgg ccggcggcgt tctcaatggt atcctgaccc gccagcaact gtgccgattc
     6841 attgaggata tcgtacttgt tggcgatgaa cagatccatc ttgtccgcgg gcagcttcag
     6901 atcgtagctg ttattgctgc tggccatgat cttgtcgaag tccgggttat accgggcaaa
     6961 gctggcgctg tccagcaggg tatatttcgc gatcacggag gaatgatatt ttccggtaac
     7021 ggcgatggtc cgggccgtgg tcagctcctt cgcggtcaga tcgcggtgga agacatacat
     7081 ggccgacccc gataactgtt cggttgcctc ttgccgggtg agtgtcgtga cactgccctg
     7141 gccttcgaac atatactggg tgccgatgaa tttctttaca tgggcgcggg attcggcggg
     7201 caggtatcgc tccagatccc agaaactgcg gctgccgctg cga
//
LOCUS       DATGFG010000147         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_771669, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000147 DATGFG010000000
VERSION     DATGFG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771669"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VKQ52_02770"
     CDS             <1..1473
                     /locus_tag="VKQ52_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HLZ86130.1"
                     /translation="DVARRRVISPPLKARLGGVSDICFSPDGKSLAVAYTRDAVAIWD
                     VDSLRTKGNLFNLEGAPMCVAFSPDGRWLAIGSGYGKNKALTLTDRANGNTRNLPMDA
                     EETQLNKLAFSPNGKILAAGRSDGKIMLWDMKGFHELRGPALLSGAGTIYGLCFDPGS
                     RLLAIGTQNKGLYTWEIDRPDPSAHFDTKRSIMDLAFIDEDNIAAVLYDSTVTVLSTQ
                     GLRPQPVLKDLVNDVMTCISLTADNRTVVLGRDRGDIWFREETSDHRISSRMLPVRLD
                     HGIKDLAIIAGSNILAVAVKDSTIRFLDVRTGREKHPPIRMDDDISFITLSHNERIVA
                     VGMNSGSIILLDAGTFQPLGNSLEGHAKVINFLRFSPDDRYLASASYDRMVYIWDVAT
                     LKPSGLPLLAHQGQVLTLAFSADGRMLASGGADNYINLWDVGKHARFASAFQANAPVK
                     KLAFSADGKYLYSAGDDRQIFRWTLNPQDWLTAAADIANN"
     gene            1485..1946
                     /locus_tag="VKQ52_02775"
     CDS             1485..1946
                     /locus_tag="VKQ52_02775"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HLZ86131.1"
                     /translation="MSTSTSTSRGATACFAFDVFISYRQLEPDLPWVRNILAPALEAP
                     GLRVCIDYRDFRLGRPVIREMERGVETSKFTLAILSPAYLESGFTELENILAQSLGLE
                     KAQRRLICMMYQPCRPRLGIRSAIWMDMTDQEKFTANLPGLIRELHSSPDE"
     gene            complement(1999..2544)
                     /locus_tag="VKQ52_02780"
     CDS             complement(1999..2544)
                     /locus_tag="VKQ52_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86132.1"
                     /translation="MNKALAVAILLLAGLGLYVYNSRRGEVKKPPPPPPPDDIADRIS
                     RDKVRAVVEKYFESLEADMEQFNIHILYSFQFDLQDLKQPYARQVQPIASLTQLGLSA
                     AEARAGKAVLIEKARAIIQGRPLDWGSSLIFLLDLSNIQSRLSALQKLYARITDKPGA
                     DSQIQKLRQAKEELISRYRTV"
     gene            complement(2541..>4070)
                     /locus_tag="VKQ52_02785"
     CDS             complement(2541..>4070)
                     /locus_tag="VKQ52_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86133.1"
                     /translation="TDLSKLGDYLSSEGRYTPFVSHVFVEVDNPMLKDLIVVDTPGLN
                     DPNELRSTLTTNFINRSSAVVYLFFSTSPLGSPDVEFIDRHLSSVSPSKIVFGMSRCD
                     LVKDVRTILEYIEDNLRTIPELRERNLLRDSKVYPVSTLAARIGQKQRAGETLTPEEQ
                     FFVRKIPRTLIDDGGLMEPFLAAIEKHIMEDKAASILDSAYQRIREVCSARIDGISGE
                     LGQLEMKVRDLQLSKEELRHKIGKTKEARLSTTDAIDEFAKRKDRVTLDISDRLSAGK
                     ESVLLGARATYSTWLAGQKVDAALKLTSFQINSILVEQITRNINKSLADHAFRELEAF
                     QSEIKQSLRDKVQAIFPVKRWEFLFTPIISIRQIIDEVLRETRDWSASLEELRVMTWG
                     LFTNKRASKINLEEGVYRFIDHTVTLFFDNLRTRINVQLLGFFEKLATDILQLLNDYE
                     NQLQGLDAEELDKTDNLETARARMELLSGHLERFGRSYASFESSIAAKWRIYNAQKPG
                     L"
BASE COUNT          839 a         1148 c         1182 g          901 t
ORIGIN      
        1 gatgtggccc ggcgccgggt gatctctcct ccgctgaagg cccggctcgg aggggtgagc
       61 gatatttgtt tcagccccga cggcaaatca ttggcggtgg cctatacgcg ggatgcggtt
      121 gccatttggg atgtggacag ccttcggaca aagggtaacc tcttcaacct ggagggggct
      181 cccatgtgcg tcgccttcag cccggacggc aggtggttgg ccattggaag cggttacggg
      241 aaaaataaag cgcttaccct aacggaccgc gccaacggaa ataccagaaa cctgccgatg
      301 gacgctgagg aaacccagct gaataagctg gccttcagcc ccaacgggaa gatcctcgcc
      361 gccggaagat ccgacggcaa gatcatgctg tgggatatga agggctttca cgagctgaga
      421 ggccccgccc tcttgtccgg tgcgggtacc atctacggac tttgcttcga tccgggaagc
      481 cggctgctgg ccatcggcac gcagaataag ggcctctaca cctgggagat cgaccggccg
      541 gatccttcag cgcacttcga tacgaagcgc agcatcatgg acctggcctt catcgatgag
      601 gacaacatcg cggcggtgct ctacgattcc acggtcaccg ttctgagtac gcagggcctg
      661 cggccgcagc ccgtactcaa agacctggtc aacgacgtca tgacctgtat ttccctgacg
      721 gcggacaacc ggaccgtggt cctcggaagg gaccggggag atatctggtt ccgggaggaa
      781 accagcgatc accgcatatc ttcccggatg ctgccggtga gactggacca tgggatcaag
      841 gacctggcga tcattgcggg cagcaatatc ctcgcggtcg cggtcaagga cagcaccatc
      901 cggttcctgg acgtccgtac gggcagggaa aagcatccac cgatacggat ggacgacgat
      961 atctctttta tcaccctgag ccacaacgag cgcatcgtgg ccgtgggcat gaacagcggc
     1021 tccatcatcc tgctggatgc agggaccttc cagccgctgg ggaattccct ggaagggcat
     1081 gccaaagtca tcaattttct gcggttcagc ccggacgacc gctacctggc ttccgccagt
     1141 tacgaccgga tggtctatat ctgggatgtt gcaacgctca agcccagcgg cctgccatta
     1201 ttggcgcacc agggacaggt gctgacattg gcctttagcg cagacggtcg gatgctggcc
     1261 tccgggggag cggataatta tatcaatctc tgggacgtcg ggaaacatgc ccggtttgcc
     1321 tcggccttcc aagcgaacgc cccggtaaaa aagctggcct tcagcgcgga tggcaagtac
     1381 ctgtattccg ccggagacga caggcagatc ttccggtgga ccctcaaccc gcaggactgg
     1441 ctgacagctg ccgcggatat tgccaacaat taaataaagg atatatgagc acatctacga
     1501 gcacatcgcg cggagccacc gcctgttttg cgttcgatgt tttcatcagc tatcggcagc
     1561 tggagccgga tctcccctgg gtgaggaata tcctggctcc cgccctcgaa gcgccgggcc
     1621 tgcgggtctg catcgactac cgcgacttcc gcctgggaag acccgtgatc agggaaatgg
     1681 aaaggggggt cgagacgagt aagtttacgc tggccatcct ctctccggcc tacctggaaa
     1741 gcggcttcac cgagttggag aatatccttg cccaatcgct gggcctggaa aaagcccagc
     1801 ggcggttgat ctgcatgatg taccaacctt gccgtccccg tctcggcatc cggtccgcga
     1861 tatggatgga tatgaccgac caggagaaat ttaccgccaa cttgcccggg ctgatccggg
     1921 aattgcattc gtcgccggat gaatgatcac ccttgcgtgg cctgtgccgg ccatcaaacg
     1981 ggttctgcgc cggcggcatc aaacggtcct gtaccggctg atcaattcct ccttcgcctg
     2041 ccggagcttt tggatttgcg aatcggcgcc tggcttatcg gtgatccggg catagagctt
     2101 ctgcaaggcc gagagcctgg attgaatgtt tgaaagatcg agcaggaaga tgaggctcga
     2161 gccccagtcg agcggccggc cctggatgat cgcgcgggcc ttctcgatga gaacggcctt
     2221 gcctgcccgg gcttccgcgg cgctcaggcc aagttgcgta agtgatgcaa tgggttgcac
     2281 ctggcgcgcg tagggctgct tgaggtcctg caggtcgaac tgaaagctgt aaaggatatg
     2341 gatgttgaat tgctccatgt cggcctcgag actttcgaaa tacttttcga cgaccgcgcg
     2401 caccttgtcc cgggaaatgc ggtcggcgat atcatcgggc gggggcggcg gcggcggctt
     2461 tttcacttcc ccgcggcggc tgttataaac atagagtccg aggccggcca gcaggagaat
     2521 ggctacggct aaagctttat tcataagcca ggtttttgtg cattgtatat tcgccatttc
     2581 gcggcgatgg acgattcaaa agatgcataa gaccgtccga atctttccag gtgcccggaa
     2641 agaagctcca tgcgggccct ggcggtctcc agattgtcgg tcttgtccaa ctcctccgca
     2701 tccaggccct gaagctggtt ttcataatcg ttcaacagct gcagaatgtc ggttgccagc
     2761 ttttcgaaga acccgagcag ctggacattg atcctggtcc tgagattgtc gaagaaaagc
     2821 gttacggtat ggtcgatgaa ccggtaaaca ccttcttcca ggttgatctt cgaagctctt
     2881 ttgttggtga agagccccca ggtcattacc cgcagctctt ccagggatgc ggaccagtcc
     2941 cttgtctccc tgagcacttc atcgatgatc tgcctgatgg aaataatcgg tgtgaacagg
     3001 aattcccagc gttttacggg aaatattgct tgcaccttgt ctcgcagcga ttgcttgatc
     3061 tcgctctgga aagcttccag ctcccggaag gcgtggtcgg cgagcgactt gttgatattt
     3121 ctcgtgatct gctccaccaa tatggaattg atctggaagc tggtgagctt taatgccgca
     3181 tccaccttct ggccggccag ccaggtggaa tatgttgccc ttgctcccag cagaacggac
     3241 tctttgccgg ccgacagcct gtcggaaatg tcgagcgtaa cccggtcttt ccttttggcg
     3301 aattcgtcga tcgcatcggt ggtggaaagc ctcgcctctt ttgttttccc gatcttatgc
     3361 cggagctctt ctttcgacag ctgcaaatcc ctgaccttca tctccagttg tcccagttcc
     3421 ccggagatgc cgtcgatcct ggcagagcat acctccctga tccgctgata cgcgctatcc
     3481 aggatcgacg ccgccttatc ttccatgata tgcttctcga tggcagccag gaatggttcc
     3541 atgaggccgc catcgtcgat gagggtcctt ggtatctttc tcacaaagaa ttgctcttcc
     3601 ggcgtcaggg tttctcctgc ccgctgtttt tggccgatgc gggccgccag ggtggaaaca
     3661 ggataaacct tactatccct gagcaggttc cgttccctaa gctccggtat ggttctcagg
     3721 ttatcctcga tatattccag gatagtccgt acatctttga caagatcgca gcggctcatg
     3781 ccaaaaacga tcttcgaagg ggacacggag gaaagatgcc tatcgatgaa ttccacgtcg
     3841 gggctgccca gcggagaggt ggagaagaac aggtatacca cggccgacga ccggttgata
     3901 aaattggtcg tgagcgtgga tcgcagctca ttgggatcgt tgaggccggg cgtgtccacg
     3961 acgataagat ccttcagcat ggggttgtcg acctccacaa aaacgtgcga cacgaatggc
     4021 gtgtacctgc cttcgctgct gagatagtct ccgagcttcg aaagatcagt
//
LOCUS       DATGFG010000148         5282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_318788, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000148 DATGFG010000000
VERSION     DATGFG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318788"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1628
                     /locus_tag="VKQ52_02790"
     CDS             267..1628
                     /locus_tag="VKQ52_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HLZ86134.1"
                     /translation="MVYKVIGLMSGSSLDGLDIAYVELQEQHSTNRQAPRTWGYQILQ
                     TACYPYPTEWKRRLAAAPGLPALDYQLLHAEYGHYLGERVLQFIEAHGLHYRVQLIAS
                     HGHTGFHDPARRMTAQLGDGAAIAATTGINVVSDLRAMDVALGGQGAPIVPIGEALLL
                     PGYEFFLNLGGIANVSRHGRGPGGADVSATHGSVGGAGVSAMQASGGPTGAAATQTSS
                     GGADAPATHRFVAFDVCPANRVLNALAADLGLEYDAEGALAAGGRVDAALLDRLNALP
                     YYGMPYPKSLANEFGTETVLPMLRQAGLSTSDALRTYVEHIAEQVGRAVGLLGWEAGA
                     AGLNPDAAEARPEAVAAERPAAGVAAMESVAWAAGVAAKMLVTGGGAHNGFLIDRLRE
                     HLAVRGVELVVPEPQLVDYKESLVMALIGVLRWREEKNVLASVTGARRDSIGGAVWIG
                     QEA"
     gene            complement(1662..2672)
                     /locus_tag="VKQ52_02795"
     CDS             complement(1662..2672)
                     /locus_tag="VKQ52_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin SocA
                     domain-containing protein"
                     /protein_id="HLZ86135.1"
                     /translation="MKSPITGKEMKRMLKDDVLVFRKEEFPILYHYYLCEDSGEEFTD
                     GALDNLNVIQAYNQYRSRYNLPFPDEIKEIREQYGVSATKMAEILGFGVNVYRHYESG
                     EVPNISNARLIHVAKDPAEFKKLVLASNVLNGNALGNTIKKIDELIASHSDFNAVGLP
                     KYLMTGLKNGKASIFTGYRTPNLDRLLEMIVYFTKECRPWKTKLNKLLFYADFGHYRK
                     TGYSISGAEYIAIQMGPVPLNFSSIFEYAAMHDDVDIAYQEFQNGGIGESFKPHPNRE
                     FNVNLFEDAECLTLKEVMNRFRDVSTADIIQLSHEEAAWSENFNDKRKISYEYAFKMV
                     NA"
     gene            complement(2990..3118)
                     /locus_tag="VKQ52_02800"
     CDS             complement(2990..3118)
                     /locus_tag="VKQ52_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86136.1"
                     /translation="MQQQKRIAKKRMNIDFKVDLLKICLNPNEKNCDPGRGGGITA"
     gene            3850..4878
                     /locus_tag="VKQ52_02805"
     CDS             3850..4878
                     /locus_tag="VKQ52_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLZ86137.1"
                     /translation="MQYTTLGNTGLIVSRLSFGAMTFTAGNKSFATIYKTEAAVADEM
                     VGRALEAGVNFFDTADAYAGGESESILGQALKSRRAEVVITTKVGNRTGTGLIQSGLS
                     RRHILWSIDQSLRRLGTDYVDVYLAHKEDPHTPLEESLEALNEVVRSGKARYIGFSNW
                     SAWRVAAAMELQRANGWAPFTHGQMHYSLLIRDIERDLLQVNQRYGLGLTVWSPLVSG
                     FLSGKYTRENMADKDNRMSGFDIMPFDREFGFRLVDRLRDIAGRHKASVAQTAIAWLL
                     AKKAVSSVIVGASKPHQLDDNLGAANVVLGVDEVTDLDALTMPAAVYPNWFGELVLDP
                     QVKEALAK"
     gene            complement(4935..>5282)
                     /locus_tag="VKQ52_02810"
     CDS             complement(4935..>5282)
                     /locus_tag="VKQ52_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HLZ86138.1"
                     /translation="TAVHFIIATENFTRFSDARKLICYCGVAPFKHESGSSIRGKTRV
                     SHLANKKLKCLLTMAAICAVRWDEDLKNKYQQKVAEGKPKMSVLNIIRAKLVERLFAV
                     IKRQSKYEIRAAA"
BASE COUNT         1155 a         1268 c         1547 g         1312 t
ORIGIN      
        1 cgtggtcttt ttacaggcgc tgaagaggag gaggcccgca gcggcgaggg ccagaacgcg
       61 ggtggaagta aatttgtttt gcatctcgct aggtttgggg caaaggaccg ccatggctgt
      121 taagggaacg ttaaggcggc atgaacgctg gattaattaa cgtggcgggc cgggagtcgg
      181 tgccaggaga tcggccatcg ggtggaggtt ggccttacga ggagtttgta gggtggcttt
      241 gcgtggcgca ttgtattttt gcccccatgg tctacaaagt catcggtctt atgagcggca
      301 gttccctcga cggattggat attgcctatg tcgaattgca ggagcagcac agcacgaatc
      361 gtcaggcgcc ccggacatgg ggttaccaga tcttacagac ggcctgttat ccctatccca
      421 cggaatggaa gcggcggctg gcggcggccc cgggactgcc ggcgctggat taccagttgc
      481 tgcatgcgga gtatgggcat tatctcgggg agcgggtgtt gcaattcatc gaggcgcatg
      541 ggttgcatta tcgggtgcag ctgatcgcga gtcatggtca taccggtttt catgatcctg
      601 cgcggcggat gacggcgcag ctgggggatg gggcggccat tgcggcgacg acggggatca
      661 atgtggtgag cgatctccgc gccatggatg tggcccttgg cgggcagggt gcgccgatcg
      721 taccgattgg ggaggcgttg ttgttgccgg ggtatgaatt ctttttgaat ttggggggga
      781 ttgccaatgt gtcgaggcat ggacgtgggc cgggcggagc cgatgtgtcc gcgacgcacg
      841 gtagtgtcgg tggggctggt gtttccgcga tgcaagccag cggtggtcca accggcgccg
      901 ccgcgacgca aaccagcagc ggcggagccg atgcgcccgc tacgcataga ttcgtggcct
      961 ttgacgtgtg tccggccaac agggtgctga atgcattggc cgcggatctg gggctggagt
     1021 atgatgcaga gggggcgctg gcggcgggtg gccgggtgga tgcggcgttg ctggaccggc
     1081 tgaatgcgtt accctattat ggaatgcctt atccgaagtc gctggcgaat gagttcggta
     1141 cggagaccgt gttgccgatg ttgcggcagg ccgggctttc gacgtccgat gcgttgcgca
     1201 cgtatgtgga gcatatcgcg gagcaggtgg gccgggccgt cgggctgttg ggctgggagg
     1261 ccggggcggc agggctcaac ccggacgctg ctgaagccag gccggaggca gtggcagcgg
     1321 agaggccggc ggcgggagtt gcggcgatgg aatccgtcgc gtgggcggct ggggttgcgg
     1381 cgaagatgct ggtcaccggt ggcggggcgc acaacggttt tcttatcgac cggttgcggg
     1441 agcatttagc ggtgcgcggt gtggagctgg ttgtgccgga gccgcagctg gtggactata
     1501 aggagtcgct ggtgatggcg ctgatcggcg tgctgcgctg gcgcgaggag aagaatgtgc
     1561 tggcgtcggt gacgggtgcg aggcgcgata gtatcggcgg cgcggtgtgg atcgggcagg
     1621 aggcgtgagg gccggagcac tgttacagcc ctttggcttt tttatgcatt caccatctta
     1681 aaagcatact cgtaactgat cttcctttta tcattgaagt tctccgacca ggccgcttcc
     1741 tcgtggctaa gttgaatgat gtccgcagtc gacacgtccc taaaacggtt catgacctcc
     1801 ttcaatgtta agcattccgc atcctcaaat aaattcacgt tgaactcccg gttagggtga
     1861 ggcttaaagg attcgccaat gccgccattt tgaaactcct ggtaggcaat atccacgtca
     1921 tcatgcattg ctgcatattc gaaaatgctg ctgaagttca aaggaaccgg acccatttgg
     1981 atcgcgatat attcagcgcc gctaatgcta tatcccgtct tccgataatg cccgaagtct
     2041 gcatagaaga gcagtttatt caacttcgtt ttccagggcc tgcattcctt tgtgaaatac
     2101 acgatcatct ccagcaaccg atccaggtta ggagtacggt acccggtaaa aatactggcc
     2161 ttcccgttct tcaaacccgt catgaggtat ttgggcaaac cgaccgcgtt aaaatcggag
     2221 tgactggcaa ttaactcgtc aatcttcttt atggtattac caagggcatt cccattcaga
     2281 acattcgacg ccagtaccaa ttttttaaac tccgcagggt ccttagctac atggatcagc
     2341 cgggcgttgg aaatgttggg cacttctccg ctttcgtaat gccggtatac atttaccccg
     2401 aaacccaata tctccgccat cttcgtcgcg ctaaccccat actgttcccg gatctctttt
     2461 atctcatccg ggaagggcag gttataccgg ctacggtatt gattataagc ctggatcacg
     2521 tttaggttat ccaacgctcc gtcggtgaat tcctcccccg aatcttcaca taaataataa
     2581 tgatagagaa tcggaaattc ctcctttcta aataccagga cgtcgtcttt cagcattctc
     2641 ttcatctctt ttccagtgat aggacttttc atataaacat ctatttaaac tgaaatcgca
     2701 ttggactgcc cgcttcgtga aaagacaggc agactacagg ggtacccgta gctcctatcg
     2761 agaattttat atataactct acgccctcat agtccgtacc gaatactgca actactttcc
     2821 acggatattt ttcgtccggg tccgggcctc cataataatc ttgtgccttt aaattatcaa
     2881 tgatctccct ctgggtcgct tcaaaattcc gaagtccgcc aaggtttggg catttttaaa
     2941 tcgttgattc atgaacaaga tcccctcttt taggatccgt accttcatct catgcagtaa
     3001 ttcctccgcc tctgccaggg tcgcaatttt tttcatttgg attcaggcaa atttttaaaa
     3061 gatcgacttt aaagtcgata ttcattcttt ttttggcaat tcgtttttgt tgttgcattt
     3121 gaacgatttt agataatgtt caactttata gttgcaaata tatgcctttt tcttaaatcc
     3181 cgaaaattta cgctattttt tgaaaaaatg tataaccttg aatattaata tattatcgct
     3241 tttagggccg ccccatgtcc tcgaatgaag tggtgctcgt tgggagaagt aatcttcctt
     3301 cggacttcat gttcgaatcg acttcttctt cgaaatgaat atgagggaag tagaggattg
     3361 gaggacgcaa aatgcgtcct ccaaagaaga taggcaaggg ctgcgatatg ccccattttg
     3421 ctttactgag caagggatta ccatgctatc ctgcgtgttg aacagtgaaa gggccatagc
     3481 ggtgaatatc caagtcatcc gagtgtttgc aaagatgcgg gagatgctgt tgaccacaag
     3541 gatattttga tcaaactcga gcaaatggaa ggaacactgt tccaacagga tgttcgaatg
     3601 aacaagcacg aagaggatat acaggcgaaa taacccgaca agagcccgaa gtttggaagt
     3661 cacaatttgc gacctccaaa aaagttaatt ataattttat tattggatgg atggcgggaa
     3721 tacgataata atagtctgtt tatcgggccc gtcgatacgc ggggagtagg tcggaaaatt
     3781 cctaacttca tcctatgaac gatcgaatgg aaaggacgca ggaacgatcg acatataaat
     3841 cttttaacta tgcaatacac aacactgggc aacacgggtc ttatcgtatc ccggctctct
     3901 tttggggcca tgaccttcac ggcgggtaac aagagtttcg cgaccattta taaaaccgag
     3961 gcggctgttg ccgatgagat ggtggggcgg gcactcgagg cgggggtcaa tttcttcgac
     4021 acggccgacg cctatgcggg cggggagtcc gagagcatcc tcggtcaggc attgaagagc
     4081 cgccgcgccg aggtggtcat cacgacaaaa gtgggtaacc gaacgggcac aggactgatc
     4141 cagtccggcc tgagcaggcg gcatattcta tggtccatag accagagcct ccggcgcctc
     4201 gggacggatt atgtcgatgt ttacctggcc cataaagagg acccgcacac gccgctggaa
     4261 gagtcgcttg aggcccttaa cgaagtcgtt cgcagcggta aggcccgcta tatcggtttc
     4321 tccaattgga gcgcctggcg ggttgcggcg gcgatggaat tacagcgggc caacggctgg
     4381 gcgccgttca cgcatggaca aatgcattat tcgctgctga tccgggatat cgaacgcgac
     4441 ctgttgcagg tgaaccagcg ctatggcctc ggcctcaccg tatggagccc gctggtgagc
     4501 ggctttctat cgggcaagta tacgcgggaa aatatggccg ataaggataa tcggatgtcg
     4561 ggcttcgata tcatgccctt cgaccgggag tttgggttcc ggctggtgga ccgcctgcgg
     4621 gacatcgccg gtcgccacaa ggcttctgtc gcacaaacgg cgatcgcctg gctgctggcg
     4681 aagaaggccg tgtccagcgt gatcgtgggt gcatcaaagc cccatcagct ggacgataat
     4741 ctcggcgccg ccaacgtggt gctgggcgtg gatgaggtga cggacctcga cgccctgacc
     4801 atgcccgccg cggtgtaccc gaattggttt ggggaattgg tgctggaccc gcaggtgaag
     4861 gaagcgttgg ccaaatagcc gatgggcccg tcgccgttga atggagcgta ttctaagtta
     4921 aaaccaaatt ttttttaggc tgcggccctg atctcgtatt tggattgcct tttgatcaca
     4981 gcaaagagac gctccaccag ttttgcccgg atgatgttga ggacactcat tttgggtttt
     5041 ccttctgcga ccttttgctg gtatttgttt tttagatctt catcccatcg gacagcgcag
     5101 atggcggcca tggtcagcaa gcatttgagc ttcttattag ccaggtgact gactctggtt
     5161 ttaccccgga tgctgctgcc agactcatgt ttgaagggtg caacgccaca gtagcagatc
     5221 agttttcggg catcggagaa gcgggtaaag ttctctgtgg cgatgataaa atgaacagcc
     5281 gt
//
LOCUS       DATGFG010000149         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1456718, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000149 DATGFG010000000
VERSION     DATGFG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456718"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..>2003
                     /locus_tag="VKQ52_02815"
     CDS             290..>2003
                     /locus_tag="VKQ52_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ86139.1"
                     /translation="MKKMLLLLLFVASLWRSSAQGQMILVNGKVTDETGKPLAGATVT
                     IKGTNISATTDETGVFHINTGNIVKPVFTISFVGYESFDYSYKGNPNFSIALHQDTRT
                     LGDVIVIGYGVQRKRDVTGATAEVKAAEIVKRPIVRVEEALQGTVPGVAVQSVNGMPG
                     AGLAVRIRGTASINNNNDPLYVIDGQIGGDISTISPNDIESIEVLKDASATAIYGSRG
                     STGVVIITTKLGREGKARIDINPWIKKEMIAKELPLMNAYQFAQATNAQYATTGQPPA
                     FSASQLQAFQSNPGTDWQHALQDKPWVQNYQVSVSGGSNDVKYMVSYLHIDQPGLILN
                     QWYRSDVARANFLIKASDRINLQFNLTGSLPHNRNTQYQGDITDPFAEAYAWDPTSPI
                     RDANGNFILESSHAALGINPIAQQTNGLNDNSGTNVTGTGIVTVRIIKGLTFTSNNSY
                     SNSSNINRQLYGPGTSTYLANSTNGFALQNSTYGHSFQNSNYFTYKGNWGDHDLTVTA
                     LYEQSSGTSYNNQSKATNLSSYSLGYWNLGLGATQNTSSGYSSQALQSYMGRVEYKFR
                     DRYLL"
BASE COUNT          515 a          691 c          432 g          365 t
ORIGIN      
        1 ctctggcaat atcaactccc caacgccggc ttcgccaccc ccatcaccta tgagatcgac
       61 ggcacccaat acatcgtcat cgcggcaggc gggggtaggg gactccccac cggcggtaac
      121 tacctcgcct tcgccctccc acactaaaaa aataccccct caccaatccc ctcgaataca
      181 accccgaccc tcgccgctga aaaaaatctt ctattaataa ggaagttaat cgataattaa
      241 agcctccgtt aaccccgccc acgttctttt gtacccaaaa cattgctgta tgaaaaaaat
      301 gctgctcctg ctgctcttcg tggcaagcct ttggagatcg tccgcccaag gtcagatgat
      361 cctggtcaac ggtaaggtca ccgacgaaac cggcaaaccc ctcgccggcg ccaccgtcac
      421 cataaaaggt actaacatct ccgccaccac cgacgagacc ggcgtcttcc acatcaacac
      481 cggcaacatc gtcaaaccgg tcttcaccat ctcctttgta ggctatgaaa gcttcgacta
      541 ctcctataaa ggcaatccca acttctccat cgcactccac caggacaccc gcaccctcgg
      601 cgacgtcatc gtcatcggtt atggggtcca acgcaaacgc gacgtcaccg gcgccactgc
      661 cgaagtaaaa gccgccgaga tcgttaaacg ccccatcgtc cgggtggaag aagctctgca
      721 gggtaccgtt cccggcgtcg ccgtccaaag cgtgaacggt atgcccggcg ccggcctcgc
      781 cgtccgcatc cgcggcaccg cctccataaa taataacaac gaccccctct acgtcatcga
      841 cggccagatc ggcggcgaca tctccaccat ctcgccaaat gacatcgaat ccatagaggt
      901 ccttaaagac gcctccgcca ccgccatcta tggctcccgc ggctccaccg gcgtcgtcat
      961 catcacgaca aaactcggga gggaaggcaa ggcccgcatc gacataaacc cgtggataaa
     1021 gaaagaaatg atcgccaaag agctccccct catgaacgcc taccagttcg cgcaggccac
     1081 caacgcccaa tacgctacca ccggccagcc tcccgccttc agcgcatccc aactccaggc
     1141 ctttcaatca aaccccggca ccgactggca acacgccctc caggataaac cctgggtcca
     1201 gaactaccag gtctccgtat ccggcggcag caacgatgtc aaatacatgg tgtcctatct
     1261 gcatatcgac cagccggggc tgatcctcaa tcaatggtat cgcagcgacg tagcccgtgc
     1321 caacttcctc ataaaggcca gcgaccggat caacctccag ttcaatctca ccggctcgct
     1381 tccccacaac cggaatacgc agtaccaggg agatatcacc gatcctttcg ccgaagccta
     1441 cgcctgggac ccaacctctc ccatccggga tgccaacggc aatttcatcc tggaatcttc
     1501 gcatgccgca ctgggcatca accccatcgc ccagcagacc aatggactca acgacaatag
     1561 cgggacgaat gtgacgggga cgggtatcgt cacggtgcgg atcataaagg gattgacctt
     1621 cacgtcgaac aacagttatt ccaatagttc gaatatcaac cgtcagctct atggacccgg
     1681 caccagcacc tatctggcca actcgacaaa cggcttcgcc ctccagaact cgacatatgg
     1741 acacagcttc cagaacagta attattttac atacaagggg aattggggcg accatgatct
     1801 cacggttacc gccctctacg aacagtccag cggcacaagc tataacaacc aaagcaaggc
     1861 aaccaacctg tcctcctatt cgctgggtta ctggaacctc ggactgggcg ctacacaaaa
     1921 caccagttcc gggtattctt cacaggccct tcaatcgtat atgggcaggg tggaatacaa
     1981 gttccgggac aggtatctcc tca
//
LOCUS       DATGFG010000150         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1227557, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000150 DATGFG010000000
VERSION     DATGFG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227557"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VKQ52_02820"
     CDS             complement(<1..808)
                     /locus_tag="VKQ52_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLZ86140.1"
                     /translation="MSSNLTIGKQEIHFLRDKIMTRYASLVGYDVRQFERGLTGTISI
                     YEKIEEVVGDYLEDESNVKRYMVFLKDSPLKLQLRKWAESGDVERKRYLRDWRSFLSA
                     SVLRKLLFYGRTESSQAYKEGVITAFYLYAHQDRQDFLQENPVPVAPERGGTIPPVRP
                     PAEPEHGSTMPVFTQEKVFLLRRLQENNSIDPNRPTLVFDGESHVLNRGNVDPENLTI
                     TSKAQAEIVFVDGEWWLEDKSVMRTTFIQVNKRTKIAKGDIIVLGNRRFLF"
     gene            complement(924..2312)
                     /locus_tag="VKQ52_02825"
     CDS             complement(924..2312)
                     /locus_tag="VKQ52_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86141.1"
                     /translation="MRKTFVFLLLMTGLCGVGRTQDMVMTQKQIKVGNSLREAQQFDN
                     SEEYLLRGLANAQHLHSQYWEAVADEYLGLLYRDLKQETNSSRYLNNALRLYTAIGMS
                     LSARVVRDILDEVSERGAGIYGGVEIGAKGVKFSVVRAWRSNGGLSFQSLRSGTNNVG
                     VISLTDNAIHDAAMVVRAFMDTLEHNSTPIPDNQIFIAVSSGVKQELDRHPGTEVKLL
                     NAIAAVAPGKTIGILTPGMEAKLTIKGIVPSNYLLRSTTMDIGSGNTKGGYFTSRTEF
                     DSLSIPWGTGTLANKLNGKSDVPGYARQFFNDSIRNNPNNPLTEELQRRSAFLDRRYA
                     FFSGGIVWVLVTYLYPEKAKEDVVEFREGDVDRFLQLVSTDAEKLRNPDLTKITDEAV
                     MQAANTDIAGTRQFNTTQLIAGAVILKGIMAEMNRVAPKKNFYFLRNGVTGWITGYIV
                     NQIAEGYSAKTD"
     gene            complement(2344..>3237)
                     /locus_tag="VKQ52_02830"
     CDS             complement(2344..>3237)
                     /locus_tag="VKQ52_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLZ86142.1"
                     /translation="KTAGVTPPQYRAPELWKNPSRGQEALTATDIWAFGASIYEMITG
                     DMPFGDLGGRMQMSDPYMRPLPAPWPEELNGILVRCLDREPGARPTAAMLEKWAGRWV
                     ETGKWKMDEMGTVMPWPAGAGGAAGADGSGGAAGVGGGVVGGATVRGAAGADARGADA
                     GGANISGTDAGGSDAGGSGVGGSGKRKSVFRVVAMAAVVGVVAVLVYLYVGWTSKPKP
                     PMGSSFGGGDSVRTAARVPAANKRDSADSVRRSAADNVRRSSLDSAARAKKGPDKPGV
                     AHQGKKDKGPAGPRLDSVKQW"
BASE COUNT          654 a         1240 c          673 g          670 t
ORIGIN      
        1 cgaacaaaaa ccgcctgttc cccaacacga tgatatctcc cttcgcgatc ttcgtccgct
       61 tgttcacctg gatgaacgtc gtccgcatca cactcttatc ctccagccac cactccccat
      121 cgacaaacac gatctccgcc tgcgccttac tggtgatcgt cagattctcc gggtccacat
      181 tcccccggtt caatacatgc gactccccgt caaagacaag cgtaggccgg ttcggatcga
      241 tggagttatt ctcctgcaac cgccgcagca ggaatacctt ctcctgggtg aacaccggca
      301 tcgtactgcc atgctccggc tcagccggcg gccgcaccgg cggtatcgtc ccaccccgct
      361 ccggcgccac cggtaccggg ttctcctgca gaaaatcctg ccggtcctgg tgcgcataca
      421 ggtaaaaagc agtgatgacc ccctccttat acgcctgcga tgactcggta cgcccataaa
      481 aaagcagctt ccgcaacaca ctcgccgaca aaaaactccg ccagtcacgc agataccgct
      541 ttcgctccac atcacccgac tccgcccatt tccgcaactg cagcttcagc gggctatcct
      601 tcaaaaaaac catataccgc tttacattgc tctcatcctc caggtaatca cccactacct
      661 cctcgatctt ttcatagata ctgatggtcc ccgtcaaacc ccgctcgaat tgccggacat
      721 catagcccac cagcgatgca tatcgggtca ttatcttatc ccgcaaaaaa tggatctcct
      781 gttttccaat cgtaagattc gatgacatat tttagttgaa tggttttaaa acagtacacg
      841 gccggcttcg ctgcgctcgc cggccccatt cgccggccgc tcggaccagc cacacccgcc
      901 ggccgcgccc ccctgccgcc ccctcaatcc gtcttcgccg aatacccctc cgcaatctgg
      961 ttcacgatat accccgtgat ccaccccgtc accccgttcc gcaggaaata aaaattcttc
     1021 ttgggcgcca cccggttcat ctccgccatg atccccttca ggatcaccgc cccggcaatc
     1081 agctgtgtcg tgttgaactg ccgcgtcccc gcaatatccg tattcgccgc ctgcatcacc
     1141 gcctcatccg tgatcttcgt caggtcggga ttgcgtagct tctccgcatc cgtactcacc
     1201 agctgcagga accggtccac atccccctcc cggaactcga caacatcctc cttcgccttc
     1261 tccggataca ggtaggtcac cagcacccag acgatcccgc cggaaaagaa cgcatatcgc
     1321 ctgtcaagaa acgcactcct gcgctgaagt tcctccgtca aaggattatt cgggttattc
     1381 cggatactgt cgttgaagaa ctgccgcgca tacccgggca catcactctt cccgttcagc
     1441 ttatttgcca gcgtccccgt cccccagggt atcgacagcg agtcgaattc ggtccggctg
     1501 gtaaaatagc ctcccttcgt attcccgctc ccgatatcca tcgtcgtact ccgcaatagg
     1561 taattcgacg gcacgatccc cttgatcgtc agcttcgcct ccatccccgg cgtcaggata
     1621 ccgatagtct tccccggcgc caccgcggca atcgcattca gtagcttcac ctccgtcccc
     1681 ggatgcctgt ccagctcctg cttgaccccg ctgctgacgg caatgaatat ctggttgtcg
     1741 gggatgggcg tggaattatg ctcgagcgta tccataaagg cccgcaccac catcgccgca
     1801 tcatgaatgg cattatccgt caggctgatg acccccacat tattggtccc gctgcgcaac
     1861 gattggaaac tgagcccgcc attgctccgc caggccctga ccaccgagaa ctttaccccc
     1921 ttcgccccga tctcgacccc gccatatatc cccgcccccc gctcggaaac ctcatccagg
     1981 atatcccgca ccacccgcgc actaagactc atcccgatag ccgtgtatag tcgcaacgca
     2041 ttgttcagat accgcgaaga attggtttcc tgtttcagat cccggtacaa caaccccagg
     2101 tactcatcgg ccaccgcctc ccagtattga ctatgaagat gctgcgcatt cgccagccca
     2161 cgcagcaggt attcctcgct gttatcgaat tgctgagcct cacgcagact gttgcccacc
     2221 ttgatctgct tctgcgtcat caccatgtcc tgcgttcgcc cgactccgca caaaccggtc
     2281 atcaaaagaa ggaaaacaaa tgtttttcgc atagaaggtt tttaaaaatg aaaaatgtat
     2341 ggttcaccac tgttttaccg agtcgagcct gggacccgca ggccccttgt cctttttccc
     2401 ctggtgggct acccccggtt tatccggccc cttcttcgcc ctggccgcac tatccagcga
     2461 cgaccgtctt acattatccg ccgccgaccg tcgcacgcta tccgccgaat cccttttatt
     2521 cgccgccgga accctggccg cggtcctgac tgaatcaccc ccgccaaaag aactccccat
     2581 cggcggcttc ggcttcgatg tccaacccac atacaggtac accagcaccg ccaccacccc
     2641 gacgacagcc gccatcgcca ctacccggaa aaccgacttc ctcttcccgg atcccccaac
     2701 acccgaaccg ccggcatccg aaccgccggc atccgtccca cttatattcg cccccccggc
     2761 atccgcaccc ctcgcatcgg cacccgccgc gccccgaacc gtcgcacccc caactacacc
     2821 tcctccaaca cccgccgcgc ccccactgcc atcggcccct gccgcacctc cagcccccgc
     2881 cggccacggc atcaccgtcc ccatctcatc catcttccac ttccccgtct cgacccaacg
     2941 ccccgcccac ttctccagca tcgccgccgt aggcctcgcc cccggctccc tgtccaggca
     3001 ccgcacaagg atgccgttca actcttccgg ccagggcgcc ggcaacggcc gcatataagg
     3061 atcgctcatc tgcatcctcc ccccgagatc gccaaaaggc atatcccccg tgatcatctc
     3121 atagatcgaa gcgccaaaag cccagatatc cgtcgccgtt agcgcctcct gcccccgcga
     3181 tggattcttc cataactcgg gcgcccggta ctgcggcggc gtaaccccgg ccgtctt
//
LOCUS       DATGFG010000151         4195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_200443, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000151 DATGFG010000000
VERSION     DATGFG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200443"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VKQ52_02835"
     CDS             complement(<1..255)
                     /locus_tag="VKQ52_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86143.1"
                     /translation="MKRINVLTWTGVSVLFLFAISCRKDIAPDATGKTSPRAATSFKV
                     MGYLPSWSGSVSSIPFSKLTHINYAFALPNSDGSYQAIDNT"
     gene            426..1106
                     /locus_tag="VKQ52_02840"
     CDS             426..1106
                     /locus_tag="VKQ52_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HLZ86144.1"
                     /translation="MPENSEFYPQVMQRYMTQPLSPVEPTDFILALLRVFEEKVFNAR
                     DFDSFPVEQIVDYIQRTHVYYLEKKLPEIEQSILLLSGHYDAQHPILIALQNFFHRYV
                     KDLSEHISAEEALLLPYINVLREADRSPLDFSRFLLHRREYSVERFLRDHHDTEDELK
                     DIRQTIRLYEPPGTNESLYRILLSQLQAFEQDLCVHAHIEEEVLIPKAKEMEQTLNEE
                     LESRSRLN"
     gene            complement(1165..3312)
                     /locus_tag="VKQ52_02845"
     CDS             complement(1165..3312)
                     /locus_tag="VKQ52_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86145.1"
                     /translation="MSQPLGPALQQEVPEVEAVSRVRDISSFLFTVNGKSFTGIQGEF
                     VDPAFLDIFSFPLTQGNKKDQLNNVYSIAITENLALRLFGTADAVGKDIRIDSVDHFT
                     VTGILTDLPANTEFAFDYLLPMAYLKKLGWTYDNWISNNIFTAVLLKPNASLEALNTK
                     IRNFTRIKAGRNDIWTHFAYPLSKWRLYSEFDNGIPVGGRIDTVRTFGVIAAFILLIA
                     CINFMNLSTARSEQRAKEVGIRKVAGAGRGLLIGQFIAEAFLTACIAGAFSLLAVELL
                     LPAFSTLVGTPLALPITSGAFWLCSLGFIVLTSLLAGSYPAFYLSSFKPVGIFRKQFR
                     KAQSVFSPRRVLVVLQFTFAVVLIVCTLIVRKQLQYAEDRDTGYSRNNLVYVNLVGDI
                     EKNYAPIRQELLNTGVATSVSKTWTPLTEGGGHYWSFRWPGENARDTNTTITVFAEDG
                     SLVKTAGMRLVAGRDIDAERYPADSLAVLLNETAVKLMGYKDPVGQILAEPYFKRNWH
                     IVGVIKDYIDASPYEQIPPIVIEGPASTLRTIHIRFDPARPTAASLAGAERIFKKYNP
                     AYPFDYAFVDLEYARKFENEQRTRTMAGLFAGLAVFISCLGLFGLSAYVAESRIKEIG
                     VRKVLGASVTGIARLLSIDFVRLVLIAILIATPVAWYAMNKWLEGYTYHISIGGGVFV
                     MAGMLAVVIALVTVSFQAVRAARANPVKSLRSE"
     gene            complement(3430..4071)
                     /locus_tag="VKQ52_02850"
     CDS             complement(3430..4071)
                     /locus_tag="VKQ52_02850"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HLZ86146.1"
                     /translation="MNRLIIPGVLVVCSLMGAAPAARPVKADWIPLFDGKTLNGWKVG
                     ENAATFSVDSGCIIAHGPTAHLFYEGDVHHHDFKDFEFKADVMTRPGSNSGIYIHTQY
                     QEGGWPGKGYEVQVNNSHTDWRRTGSLYGIEDIREVYVKDDVWFTEYVSVRGKHIVIK
                     INDKTVVDYTEPDNVKRPAGSEGRVLSSGTFALQGHDPNSKVYFRNILVKPLD"
BASE COUNT         1061 a         1337 c          917 g          880 t
ORIGIN      
        1 cgtgttgtcg atagcctggt aactgccatc cgaattgggt agcgcaaaag cataattgat
       61 atgggtcagc ttgctgaagg ggatcgaaga gacactcccc gaccaggaag gaagataccc
      121 catcacctta aaggaagtcg ccgcccgggg agaagtcttg ccggtggcgt cgggggcgat
      181 atccttccgg caggagatgg caaacaaaaa aaggaccgat acaccggtcc acgttaacac
      241 attgattctt ttcatggtag aataggttta aaaaatgaat atgtaggcag tcagggggat
      301 aaggcccaaa accccttgcc cgccaaatgt aagacacccc gttccgcaag ccaatacccc
      361 taaacccgta taaaaattca ggatttatct taaatttgtt caccagccgt aaaagcagga
      421 atgctatgcc cgaaaacagt gagttttatc cccaggtgat gcagcgctat atgacccagc
      481 cgttgtcgcc ggtggaaccg accgatttta tacttgccct gttgcgtgtg ttcgaagaaa
      541 aagtattcaa cgccagggat tttgactcct ttcccgtaga gcagatcgtg gattacatcc
      601 agcgaaccca cgtatattat ttggagaaaa agctaccgga aatagagcag agcatcctat
      661 tgctctccgg tcactacgac gcgcagcatc ccatcctcat cgccttgcag aatttcttcc
      721 atcgctatgt aaaggacctc tccgaacata tcagcgcgga agaagccttg ctcctcccct
      781 atatcaatgt attacgcgag gcagaccgca gccccctcga cttctcgcgc tttctgctcc
      841 accgcaggga atactcggtg gaacgcttcc tccgcgatca ccacgatacc gaggatgaac
      901 taaaggatat ccgccagacc attcgcctct acgagccgcc gggaacgaac gaatccctct
      961 atcgcatcct gctcagccaa ctccaggcct tcgaacagga cctctgcgtc cacgcacaca
     1021 tcgaagagga agtcctcatc cccaaggcca aagagatgga acaaaccctc aacgaagaac
     1081 tggaatcccg ctcccgcctg aactgatccc ggacgatcac cacaattagc tcactcgcta
     1141 cgcaatccca aagccgcgta gcggctactc gctacgcagc gacttcaccg ggttcgccct
     1201 cgcggccctc accgcctgga aactcaccgt caccagcgcg atcaccaccg ccagcatccc
     1261 cgccatcaca aacacccccc cgccgataga gatatgatag gtataccctt ccaaccattt
     1321 gttcatcgca taccaggcca ccggcgtcgc gatcaggatc gcgatcagca ccaaccgcac
     1381 aaaatcgatc gacaataacc gcgcgatccc cgtcaccgac gcccccagca ccttccgaac
     1441 cccgatctcc tttatccggc tctccgcgac ataggcgcta agcccgaaca aacccagaca
     1501 ggagataaaa acggccagcc cggcaaacaa acctgccatc gttcgcgtcc gttgctcatt
     1561 ctcgaatttg cgggcatatt ccaggtcgac aaaggcataa tcgaaaggat aggccgggtt
     1621 gtatttttta aagatccgct ccgcacccgc gagactcgcc gccgtcggac gggcagggtc
     1681 gaaccggata tgaatcgtcc gcaacgtcga agccgggcct tcgatgacaa tcggcgggat
     1741 ctgctcatac ggcgacgcat cgatataatc ttttatgacc ccgacgatat gccagttccg
     1801 cttgaaataa ggctccgcaa ggatctgccc caccggatct ttatatccca tcaattttac
     1861 cgccgtttca ttcaacaaca cggccaggct gtcagcagga taccgctccg catcgatatc
     1921 cctgccggcg acaagccgca tcccggcagt cttcaccagg ctgccatctt ccgcgaaaac
     1981 cgtgatcgtc gtattcgtat cccgcgcatt ctctcccggc cagcgaaagc tccagtaatg
     2041 cccgccgccc tcggtcaggg gcgtccaggt cttgctcacc gacgtggcga ctcctgtatt
     2101 cagcagttcc tgccttatcg gcgcataatt cttttcgata tccccgacaa ggttcacata
     2161 gactaaatta ttcctggaat acccggtatc ccgatcctcg gcatattgca attgcttccg
     2221 cacgatgagc gtgcatacga tcagaacaac ggcaaaagtg aattgcagca ccacaagcac
     2281 cctccgggga gaaaacaccg actgcgcctt gcggaattgc tttcggaaga tacctaccgg
     2341 tttgaacgag gaaagataga acgccggata actgcccgcc agcagactcg tgagcacgat
     2401 aaagcccagc gaacaaagcc agaaagcgcc actggtaata ggcagcgcca gcggtgtccc
     2461 caccagcgtg ctaaaggccg gtagcaacag ctccaccgcc agcagcgaga aggcccccgc
     2521 gatacaggcc gtaagaaacg cttcggcgat gaattgaccg ataagcaacc ctctgcccgc
     2581 accagctact ttccggatgc ccacttcctt cgcccgctgc tcgcttctcg cagtgctcaa
     2641 attcataaaa ttgatacagg cgatcagcag gataaacgcg gcgatgacgc caaaggtccg
     2701 caccgtatcg atccggccgc caaccggtat gccattgtcg aactccgaat acaaacgcca
     2761 tttggaaaga ggataggcaa aatgcgtcca gatatcgttc cgccccgcct ttatgcgcgt
     2821 aaaattcctg atcttcgtat tcaacgcctc caggctcgca ttgggcttca gcagtacggc
     2881 cgtgaaaata ttgttgctga tccagttgtc ataggtccag cccagcttct tcagataggc
     2941 catcggcaag agatagtcga atgcaaactc cgtattcgcc ggcaggtcgg taagaattcc
     3001 cgtcaccgta aaatgatcga cgctgtcgat ccggatatcc ttcccgacgg catccgcggt
     3061 tccaaacagc ctcaacgcca ggttctcggt gatcgcgatc gagtacacat tgttcagctg
     3121 gtccttttta ttaccctgcg tcaacggaaa gctaaagatg tccaggaagg ccgggtcgac
     3181 aaactccccc tggatacccg taaagctctt gccattcacc gtaaaaagga aactgctgat
     3241 atcccgtacc cgcgagaccg cctccacctc cggcacctcc tgctgcaaag caggccccag
     3301 cggctgcgac acaacaccgt acccatgggc ctcaattccc ctgccaactt acaaaccctt
     3361 catcctcagt accgacctca cccaccactg tccgccaccg aacagttccc tgtccgccgc
     3421 cgctacactc taatccaacg gcttcaccag gatattccgg aaatagacct tgctgttcgg
     3481 atcatgcccc tgcaacgcaa atgttccgct cgacaacacc ctgccctcac tgcccgccgg
     3541 ccgcttcaca ttatccggct cggtataatc caccacggtc ttatcattga tcttaatgac
     3601 gatatgcttt ccgcgcacac tcacatattc cgtaaaccag acatcgtctt tcacatacac
     3661 ctccctgata tcctcgatcc cgtacaaact cccggtcctg cgccaatccg tatgcgaatt
     3721 attcacctga acttcatacc ccttgcccgg ccatccaccc tcctggtatt gtgtatgaat
     3781 ataaataccc gaattgctcc cgggccgcgt catcacatcg gccttgaact cgaagtcctt
     3841 gaaatcatga tggtgcacat ctccttcata gaaaagatgc gccgtcgggc catgcgcaat
     3901 gatacagccg ctgtcgacgg aaaacgtggc cgcattctcc cctaccttcc atccgttcaa
     3961 cgtcttccca tcgaagagcg ggatccaatc ggcctttacc ggccttgccg cgggtgcggc
     4021 tcccattaac gagcaaacaa caagaactcc cgggatgatc aacctattca tattaaatca
     4081 tattagtatg cattaaatat atcgaaatct ttctttagca aatcgacaat tattatcttt
     4141 cgtgccgaat ccaatatcaa atgtcatgac ctccctcaag aagttattcc ttatc
//
LOCUS       DATGFG010000152         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1342147, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000152 DATGFG010000000
VERSION     DATGFG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342147"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VKQ52_02855"
     CDS             complement(<1..213)
                     /locus_tag="VKQ52_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86147.1"
                     /translation="MKRFLLLPLYLLIGFAPGYAQSTIGLPAIRSYTLADYHAAIEIW
                     DIGQDRRGVLYFANNDGLMTFDGNYWK"
     gene            347..3013
                     /locus_tag="VKQ52_02860"
     CDS             347..3013
                     /locus_tag="VKQ52_02860"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HLZ86148.1"
                     /translation="MYKTYFKYLSLVILLVLCQAGAGAQSFRVLVVASRARDHQKMIA
                     AARPFFEKMAAEESFQLDFTDDTSQINPINLARYQVFVMLHLAPFDMSYAQQDALRQF
                     VEEGKGWVGIHAAGLTGRQFHPNDRYWQWFEDLMGNVLYSPHPAYQHATLVVEDRAHP
                     VMRHLPARLDIPDEWYEWDKSVRGNPHVHVLASVNESTYHQNKPMGDHPVIWTNDNYR
                     RMIYISPGHDPELLRDPAYAGLLRDAIRWAASSGPAGASLPLSDRRVGYEGRYTLGNP
                     GAPQSELFIRLKQVLARPAFIAAAPSATAPRFTITTADSAAGTITGKGQLDIATNDPG
                     HHYQITFDFNIAVTDGQYSFRTDHYFEKPIGIGTTSEYTKIEYRWWDFRQGHPWHRED
                     QRLFTGLDSAMNTVMDSLYKKVDLPRFRALVLFENGGWHVNYSWRARNWLAQQAVDSN
                     FAIDYLTHTDSINDELLSRYQLIIQLDFVPYGWKPAAEAAFKRYIEEGRGGWVGFHHA
                     TLLGEFDHFPMWPWFHAFMGGIRWKDYIARFAKATVRVEDHRHPVFQGIPDSFVVQKE
                     EWYTYDKSPRPNVHVLAHVDESTYYPDTAVKMGDHPVIWTNEKVRARNVYIFMGHDLV
                     LFDDSVYRRLFANAISWAAATPPYPSSAVVPVPAHPRYHVLAFYSNTVETDHVDFARD
                     AIRFYAGLAARNNFAFDTTSNWGNCNDTLKKYQVVLWLNDFPHTEEQRSAFQQYMEHG
                     GAWLGFHVSGYNDRDTHWPWFVHFFGDAVFYNNSWPPLPAKLIVDDNKHPATHRLPSR
                     YTAPLNEWYGWKPNPRDNKDIRVLVTLDPANYPLGKKDTIHGGDIPVVWTNTKYKMLY
                     MNMGHGDQNLTSPTQDHLFEDALEWLGGAARR"
     gene            3087..>3448
                     /locus_tag="VKQ52_02865"
     CDS             3087..>3448
                     /locus_tag="VKQ52_02865"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HLZ86149.1"
                     /translation="MPTTETIIPFSKGKLSRLLLLAVGCILLGIVFVIIQSHEPGVFL
                     TLPILAALIGAFLLLVAFLLGTIYFRQILKNGPGLVIDITGFTDYSSSLAAGYIPWTD
                     VKAIKTITLPKDKQKYISV"
BASE COUNT          728 a         1122 c          864 g          734 t
ORIGIN      
        1 cttccagtaa ttcccgtcaa aggtcatcaa cccgtcattg ttggcgaagt atagtacccc
       61 tcgccggtcc tgaccgatat cccagatctc gatcgccgca tgatagtcgg caagggtata
      121 gctccggata gccggtaacc cgatcgtcga ctgggcataa ccaggtgcga acccaatcag
      181 caggtacaat ggcaggagca ggaagcgctt catcggacga caagttatcg aaaaacagcc
      241 gatgtagtgt taatgtaggg gcaaacaaat tgcccgtctg gccggtgaac cggatctttg
      301 ctccgacggt ttagcctccc ttgccactaa ctaatttgct gggcctatgt acaaaacata
      361 ttttaagtat ctctccttag taatcctgtt agttctatgt caagccggcg cgggcgctca
      421 gtcatttcgt gtcctggtag tggcttcgcg agcccgtgat caccaaaaaa tgattgccgc
      481 cgcccggcct tttttcgaaa aaatggccgc cgaagagtcc ttccaactgg acttcaccga
      541 cgacaccagc cagatcaacc ccatcaacct ggcccgctac caggtcttcg tcatgctcca
      601 tctggcgccg ttcgatatgt cctatgccca gcaggacgcc ctgcggcagt tcgtcgaaga
      661 gggcaagggg tgggtgggta tccatgccgc cggcctcacc gggcgccagt ttcatcccaa
      721 cgaccgttac tggcaatggt tcgaggatct gatgggcaat gtcctctact ccccccatcc
      781 tgcctaccag catgccaccc tcgtcgtcga ggaccgcgcc catcccgtca tgcggcatct
      841 gcccgcccgg ctcgacattc ccgacgaatg gtacgaatgg gacaaaagcg tccgcggcaa
      901 tccccatgtc catgtcctgg cctcggtgaa tgaatccact taccaccaga ataaaccgat
      961 gggcgatcat cccgtcatct ggacaaatga caactatcgc cggatgatct atatcagccc
     1021 cggtcacgac cccgaactcc tgcgggatcc cgcctatgcc ggtcttctcc gggatgccat
     1081 ccgctgggcc gcctcttcgg gacccgccgg cgcctccctc cccctgagcg atcgccgtgt
     1141 cggctacgag gggcgctata ccctgggtaa ccccggcgcc ccgcaatcgg aactctttat
     1201 ccggctgaag caggtcctgg cccggcccgc cttcattgcc gccgccccat ccgccaccgc
     1261 cccccgcttc accatcacga cggccgactc cgccgccggc acgatcacgg gtaaggggca
     1321 gctcgatatc gctacgaacg acccgggtca ccattatcaa ataaccttcg atttcaacat
     1381 tgccgttacc gacggccaat acagcttccg caccgaccac tacttcgaaa aacccatcgg
     1441 catcggcacc acatccgaat acacgaagat cgaatatcgc tggtgggact tccgccaggg
     1501 ccacccctgg catcgcgagg atcagcggct cttcacgggg ctcgactcgg ctatgaacac
     1561 ggtgatggac agcctgtaca aaaaggtgga ccttccccgc tttcgcgccc tggtgctctt
     1621 tgagaacggc ggctggcatg tcaactattc ctggcgcgcc cgcaactggc tcgcacagca
     1681 ggcggtggac agcaatttcg ccatcgacta tctgacgcat accgacagca tcaacgatga
     1741 attattgagc cgctaccaac tgatcatcca gctcgacttc gttccctatg gctggaaacc
     1801 cgcggccgaa gcggccttca aacggtatat cgaagagggg aggggaggct gggtcgggtt
     1861 ccatcatgcc accctgctgg gtgagtttga ccatttcccg atgtggccct ggttccatgc
     1921 gtttatgggc ggcatccgct ggaaggacta tatcgcccgt tttgcaaagg ccacggtccg
     1981 ggtggaagat catcgtcatc cggttttcca gggtatcccc gattcttttg tagtgcagaa
     2041 ggaggaatgg tacacctatg acaagagccc ccggccgaat gtccatgtcc tcgctcacgt
     2101 cgacgaatcc acgtattacc cggatacggc ggtgaagatg ggcgatcacc ccgtgatctg
     2161 gaccaacgaa aaggtccgcg ctcgcaatgt ctatattttc atgggccacg acctggtgct
     2221 cttcgacgat agtgtataca ggcgcttgtt tgcaaatgcc atctcctggg ccgcggccac
     2281 tccgccttat ccttcctctg ccgtcgtccc tgtgcctgcg cacccccgtt accatgtcct
     2341 cgccttctat tccaatacgg tggagacgga tcatgtcgac ttcgcccgcg acgccatccg
     2401 cttttacgcc ggactggccg cccgcaacaa cttcgccttc gataccacgt ccaactgggg
     2461 caattgcaac gataccctga aaaaatacca ggtcgtcctc tggctgaacg acttccccca
     2521 taccgaggag cagcgcagcg ccttccagca gtatatggaa catggcgggg catggctggg
     2581 ttttcacgtg tcaggctata acgatcgcga tacccactgg ccctggtttg tccacttctt
     2641 cggcgacgcg gtattctata acaacagctg gccgccgctc ccggcgaagc tgatcgtcga
     2701 cgacaacaaa catccggcca cacaccgcct gccatcgcgc tataccgccc cgctgaacga
     2761 atggtatggc tggaagccca acccccgcga caacaaagac atcagggtcc tggtgaccct
     2821 cgatccggca aactatcccc ttggcaagaa ggatactatt catggcggtg atatccccgt
     2881 cgtatggacg aatacgaaat ataagatgct gtacatgaat atgggccatg gcgaccagaa
     2941 tctcacttcg ccaacccagg atcacctttt cgaggacgcc ctcgaatggc tgggcggggc
     3001 cgctcgccga taagaccccc cgatttgccg acgggggctg gcataaatcg ggcaaaatgt
     3061 ttatcttggg gcgcgcaagc tgttccatgc ctaccaccga aaccattatc ccgtttagca
     3121 agggcaagct atcccggctt ctcttgctcg ctgtagggtg tatcctgctg ggtatcgtct
     3181 ttgtcatcat ccagtcccac gagcctggtg ttttcctgac cttacccatc ctcgctgccc
     3241 tgatcggcgc cttcctcctc ctcgtcgctt tcctgcttgg aacgatctat ttccgccaga
     3301 tcttaaagaa tggcccgggc ctcgtcatcg acatcaccgg cttcaccgat tactccagca
     3361 gtctcgccgc gggttatatc ccctggaccg acgtcaaagc cataaaaacc atcacgcttc
     3421 ccaaagacaa acagaaatac atctccgt
//
LOCUS       DATGFG010000153         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_87741, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000153 DATGFG010000000
VERSION     DATGFG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87741"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VKQ52_02870"
     CDS             complement(<1..789)
                     /locus_tag="VKQ52_02870"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HLZ86150.1"
                     /translation="MKFSISPFTTLLALTGLIAPATAQRPATAEGGNRAGSETRPAAS
                     IASVTQNMKRSEGFYTFYYDEKTGRIMLELDRFDQEFLYFSSLPEGIGNGGAERGQAS
                     AVIAKFIRVGPKVFLLQPNYSQRSVNGNADMQKDVEDGFSQSILFGFSPVAVEGDKLL
                     IDATPFIVRDALHIGENIGSGRGNPGSAFSAAANAPGRAGAAAGGGYRLDESRSAVFM
                     GNTKNFPKNTEFEALVTFAGGTAGGAGGGFRRGGGVAPDPTSVTV"
     gene            1232..2101
                     /locus_tag="VKQ52_02875"
     CDS             1232..2101
                     /locus_tag="VKQ52_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ86151.1"
                     /translation="MFLAIPFRLSRGTLFLLTTVIATGLYAQPAKADTFILHFGFDRS
                     EVRPVDSGALRSFIGDRLLSSSDIDSIGITGYTDTVGTPEYNDRLSLRRATTTAVLFR
                     QWLGSASSLPVRVEGRGEADALPGDDSGSRRVTIICWHRPPPPPVVAKVDTPRITSEP
                     DTVFELDDIRFFANTTNLTEEARLALPRRIDYLLSLKDRFLEIDGYCNSPGPPLSASD
                     PLYVLSVKRAKFIYDCLVERGFDPDRLVYKGKGNTNPRNAHPTTRGEMDQNMRVEIRV
                     YKKRPATSALDFN"
     gene            2172..3374
                     /locus_tag="VKQ52_02880"
     CDS             2172..3374
                     /locus_tag="VKQ52_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLZ86152.1"
                     /translation="MKLRTKAMLLGAAAVIGSYFLFANGNKPVNSILDEPKAPIVKTA
                     YTAEPAVQPVDFEKAASAAVPSVVHIKTTTKFKEVAGRQGGQDDDPSGGMDGDLFRRF
                     FGDGGGRMMPQPDQKASGSGVIISSDGFIVTNNHVVNGATKITVTLDNRRNYTAKVIG
                     TDPNTDLALIKIDGRNLPVMNIGNSDDVKLGQWVLAIGYPLNLDVTVTQGIVSAKSRN
                     IGINTQASAPVESFIQTDAAVNPGSSGGALVNTNGELVAINSAIASPTGAFAGYAYSI
                     PSNLMKKVVGDIMKYGSVQRGYLGISMAPEGLDDARKKELGINDDVDGVYVIEVDPQG
                     AAGEAGIRKGDVIVKIDGQAVNSDARLAELIARQKPGDKIRITYVRGGEQHESEATLK
                     NKPGSFAK"
     gene            complement(3388..>3660)
                     /locus_tag="VKQ52_02885"
     CDS             complement(3388..>3660)
                     /locus_tag="VKQ52_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86153.1"
                     /translation="RKVMGASVTDLWALLSKDFVLLVGISLLIAVPLGRYFMGNWLMH
                     YQYRSTMPWWVFASAAIGALLITLLTVSYQGIRAARMNPVKALRTE"
BASE COUNT          777 a         1021 c         1067 g          795 t
ORIGIN      
        1 caccgttacc gaggttgggt cgggcgccac gccgccgccg cgacggaaac cgccaccggc
       61 cccaccggcc gtgccgccgg caaaggtcac cagcgcctcg aactcggtgt tctttggaaa
      121 gttctttgta ttccccatga agacggcgct gcgggactca tccagccggt agccgccacc
      181 cgccgccgca cctgcgcgac ccggagcatt ggccgcggca gagaaggcgc tgccgggatt
      241 accgcgaccc gacccgatgt tctcgccgat atgcagtgca tcgcgaacga taaacggcgt
      301 cgcatcgatc agcagtttat ccccttcgac ggccacgggt gagaaaccga agaggatgga
      361 ttgcgaaaag ccgtcttcca catccttttg catgtccgca tttccgttga cggaacgctg
      421 gctgtagttg ggttgcaaca ggaatacctt cgggcctacc cgaatgaatt tggcgatcac
      481 ggccgaagcc tgtccgcgtt ccgcgccgcc gttgccgatg ccttcgggca gggagctgaa
      541 atagaggaac tcctggtcga agcgatcgag ttcgagcatg atcctgccgg tcttctcgtc
      601 gtagtagaag gtgtagaagc cttcggatct cttcatgttt tgtgtcacgg atgcgatgct
      661 ggccgcgggt cttgtttcgg agcctgcgcg attgccgccc tcggccgtgg cgggtcgctg
      721 tgcggtcgcg ggggcgatca ggccggtgag ggcgaggagg gtggtgaatg gtgagatcga
      781 aaacttcata tgcagtagaa ttgttttatg tttctcatga cgtatgggtc cggcaggcaa
      841 gagtacgcca taatcccggc tgtcccagcg gattatgact aacggtcgat tcgttccatg
      901 gatcggggga ttagggattc tttaggttat cggatcgtta accagccgat tgggaagtta
      961 aactcctttc gccggattcc ctcttactta tgaacttaaa atttacgatc atgaaaacgt
     1021 caaagtatct caagctcttt ttgctgggtc tcgttatagc cgctacgttt agttcctgtg
     1081 ttgtatatga gcacccctac caccatcact atggttacta tcgttagaaa atcggtgtcc
     1141 gtaacgggtt cccaactggg acgggaagct cctcactcgt cgtaccgtga gatccgcttc
     1201 ccgtcccttt cttttttatt atcttgtgta catgtttctt gccattccct ttcgcctgtc
     1261 ccgggggact ctttttctat tgacgacggt catcgccacg ggcctgtatg cccaaccggc
     1321 aaaggccgat accttcatcc tccattttgg ctttgaccgt agtgaagtgc ggcccgtgga
     1381 cagtggggcc ctgcggtcat ttatcggcga ccgccttctt tcctccagcg atatcgactc
     1441 catcggtatt acggggtata ccgacaccgt cggcaccccg gagtacaacg accggctctc
     1501 gttgcgccgg gccacgacga cggcggtcct cttccggcag tggctgggct ccgcatcttc
     1561 cctgccggtg cgggtcgagg gtcgggggga agccgatgcc ctgccagggg atgacagcgg
     1621 gagccggcgg gtgaccatta tctgctggca tcgtcctccg ccgcccccgg tggttgccaa
     1681 agtggatacg ccacgcataa catcggagcc tgacacggtg ttcgaactcg acgatatccg
     1741 cttttttgcc aatacgacga atctcacgga agaagcaaga ctcgccctac ccaggcgtat
     1801 cgactatctg ctttcgctaa aggaccgctt cctggagatc gacggctatt gtaattcgcc
     1861 gggaccgccg ttgtctgcca gcgatccgct ctatgtcctt tcggtcaagc gggcgaaatt
     1921 catctacgac tgccttgtcg agcggggctt cgatcccgac aggcttgttt ataagggcaa
     1981 gggaaatacg aatccgcgca atgcccatcc taccacgcgc ggggaaatgg accagaatat
     2041 gcgggtcgag atccgggtgt ataaaaaaag gccggcgacg tctgctttag attttaatta
     2101 acaccggcgt gggaacgatc catctacgaa ggtgttagta tatattatta cacatctaaa
     2161 agcaagacat catgaaattg agaacaaagg caatgcttct cggcgccgcg gccgtgatcg
     2221 ggagctattt tttattcgcc aatgggaaca agccggtgaa cagcatactg gatgagccga
     2281 aggcgcccat cgtgaagacc gcctatactg ccgaaccggc agtgcaaccg gtagattttg
     2341 aaaaggccgc gtcggccgcc gtcccctccg tcgtacatat caagacgacg acgaagttca
     2401 aggaagtcgc gggcagacag ggtggtcagg acgacgatcc gtctggtggg atggatggcg
     2461 acctcttccg ccgtttcttt ggtgatggcg gaggcaggat gatgccgcag ccggaccaga
     2521 aggcttccgg ttcgggggtc attatcagca gtgacggatt tatcgtcacc aataaccacg
     2581 tggtcaacgg cgctaccaag atcacggtga cgctggacaa tcgccggaac tataccgcca
     2641 aggtgatcgg taccgatccg aacaccgatc tggcgttgat caagatcgac ggcaggaacc
     2701 tccccgttat gaatatcggc aactccgatg atgtgaaact gggtcagtgg gtgctcgcca
     2761 tcggttaccc gcttaacctg gatgtcaccg ttacccaggg gatcgtaagc gccaagtcga
     2821 ggaacatcgg tatcaatacc caggccagtg cgcccgtcga atcgtttatc cagacggatg
     2881 cggccgttaa cccgggtagc agtgggggcg ccctggtgaa caccaatggg gaactcgtcg
     2941 ccatcaactc cgccatcgct tcgcctaccg gggcttttgc gggctacgcc tatagcattc
     3001 cgtccaacct gatgaagaag gtggtcggcg atatcatgaa atacggctcg gtacaaagag
     3061 gttatctcgg tatcagcatg gcgcccgaag gccttgatga tgcaaggaag aaagagctgg
     3121 ggatcaatga cgacgtggac ggggtatatg tcatcgaagt agacccgcag ggggctgccg
     3181 gtgaggccgg gatcaggaag ggtgatgtca tcgtgaagat cgatggtcag gccgtgaatt
     3241 cggatgcccg tctggccgag ctgatcgccc gccagaagcc tggtgacaag atcaggatca
     3301 cctatgtccg gggcggtgaa caacacgaat cggaggccac tttaaaaaat aagccaggca
     3361 gctttgcgaa atagtcttcg gcaagatcta ctcggtgcgc agggccttta ccgggttcat
     3421 tctcgcggcc cggatcccct ggtagcttac cgtcagcagc gtgatcagca gggccccgat
     3481 cgcggccgat gcaaagaccc accagggcat ggtcgaccgg tattgataat gcatcagcca
     3541 gttgcccatg aaatagcggc cgaggggcac ggcgatcagc agtgagatac ctaccagcag
     3601 gacgaagtct ttgctcagca aggcccatag atcggtcacc gacgccccca tcacctttct
//
LOCUS       DATGFG010000154         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1000916, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000154 DATGFG010000000
VERSION     DATGFG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000916"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2308)
                     /gene="alaS"
                     /locus_tag="VKQ52_02890"
     CDS             complement(<1..2308)
                     /gene="alaS"
                     /locus_tag="VKQ52_02890"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HLZ86154.1"
                     /translation="MNQFKDYFLGNKKAPHSRVADTQKCLRVSGKHNDLEEVGVDTYH
                     HTMFEMLGNWSFGDPADAAAGYFKKEAIAWSWELLTAEYGIPKDRLYVTVFEGDKESG
                     LPQDSEAFEEWKKWIAPERILLGSRKDNFWEMGDTGPCGPCTEIHVDCRTDAERKEQD
                     GATLVNNDHPQVIEIWNNVFMQFNRLKDGSLQPLPAKHVDTGMGLERLVRVLQEKHSN
                     YDTDLFTGTIHQVAAIVKREYTAGDSKQDVAFRVIADHLRAISFTIADGQLPSNTEAG
                     YVIRRILRRAVRYYYSYLDYKQPLLFQLVPVLAAQFRDVFPELQHQAEFVAKVVKEEE
                     ESFLRTLEKGLKKMEDIVQARKKAGSAVIDGSAAFELYDTFGFPEDLTALIGRENGLE
                     VDKKGFEAEMQQQKARSRAATAIDTEDWVVLVDNPLVEFVGYDSLETNTQIVKYRKIK
                     AKGRESWQLVLEATPFYAESGGQVGDTGTLHSKGEEIAVLDTKKENDLIVHFTDKLPA
                     DVESPVIATVNRPKRKATEVHHSATHLLHAALRKVLGTHVAQKGSLVNDGQLRFDFSH
                     FAKMTEEEIAAVEVLVNEKIRENIPVIIRQMPKDEALKLGAMALFGEKYGDVVRVVMI
                     DPGYSVELCGGTHVGATGELGFFKIRHEAAVAAGVRRIEAVSGPAAEAFLTEQFAELR
                     AVREALKNPTQLGRAAETLVAENSELKKKLEKAEARQLAELRQQLLQKVQTLGGVAFI
                     GEVVEAGSADAVKKLCFDLKAGMAALPGT"
BASE COUNT          417 a          728 c          639 g          540 t
ORIGIN      
        1 gcgtgccggg aagggccgcc atacccgcct tcaggtcgaa acataatttt tttaccgcgt
       61 cggcgctgcc cgcctcgacg acttccccga taaaagccac cccgccaagg gtctgcacct
      121 tttgcaggag ttgctgccgg agttcggcca gttgtcttgc ctcggccttt tcgagtttct
      181 tttttagctc gctgttctca gccacgagcg tctccgcagc cctgccaagc tgtgtgggat
      241 tcttgagggc ctcgcggacg gcacggagct cggcgaattg ttcggtgaga aaggcttccg
      301 cggcaggacc actgacggcc tcgatgcggc gaacgccggc ggcaacggcc gcttcatgcc
      361 ggatcttgaa gaaccccagt tcgcccgtgg cgccgacatg cgtaccgccg cagagctcga
      421 cggaatagcc ggggtcgatc atcactaccc ggacgacatc gccatatttt tcgccaaaaa
      481 gggccatggc gcccagcttc aaggcctcat ccttcggcat ctgccggatg atgacgggaa
      541 tgttctccct gatcttttca ttgacgagga cctcgacggc ggcgatctcc tcttcggtca
      601 tcttcgcaaa atgcgagaag tcgaaacgca gctgcccgtc gttgacaagc gaacccttct
      661 gggccacatg ggtgcccaat accttgcgca gcgcggcatg cagcagatgc gtggcggagt
      721 ggtgcacctc cgtcgccttt cttttcggcc ggttcacggt cgcgatcacc ggcgactcca
      781 catcggccgg cagcttatcc gtaaaatgaa cgataagatc attttccttt ttcgtatcga
      841 gcacggcaat ctcctctccc ttcgaatgga gggtaccggt gtcgcctacc tgcccgccgc
      901 tctcggcata gaagggggtg gcttccagca ccagctgcca ggattccctg ccctttgctt
      961 ttatcttccg gtatttgacg atctgggtgt tggtctcgag cgagtcatac ccgacgaatt
     1021 ctaccagcgg gttatcgacc agcaccaccc agtcctcggt gtcgatggcc gttgcggccc
     1081 tcgagcgggc cttttgttgt tgcatttccg cttcgaaccc cttcttatcg acttccagtc
     1141 cgttctcccg gccgatcagc gccgtcaggt cttcggggaa accaaaggtg tcgtacagct
     1201 cgaaggcagc cgagccgtcg atgacggcgg aacccgcttt cttccgggcc tgcacgatgt
     1261 cctccatttt cttcagcccc ttttccaggg tccgcagaaa ggattcctct tcttccttca
     1321 ccaccttggc gacgaactcc gcctggtgct gcaattccgg gaagacatcc ctgaactggg
     1381 ccgccagcac gggaaccagc tgaaagagca gcggctgctt atagtccagg taggaataat
     1441 agtagcggac ggcccgccgc aggatgcggc ggatcacata gccggcttcg gtattggagg
     1501 gcaactgccc gtcggcgata gtaaaactga tcgcccggag atggtcggcg atcacccgga
     1561 aagcgacatc ctgtttactg tcgccggccg tatattccct tttgacgatg gccgccacct
     1621 ggtggatcgt gccggtgaaa aggtcggtat cgtagttcga atgtttctcc tgcagcaccc
     1681 ggaccagccg ctcgagcccc atgccggtat cgacatgttt cgccggcagg ggttgcagac
     1741 tgccgtcctt gaggcggttg aactgcatga aaacgttgtt ccagatctcg atcacttgtg
     1801 gatgatcgtt attcaccagc gtcgccccgt cctgctcctt ccgctccgca tcggtacggc
     1861 aatcgacatg gatttcggta cagggtccgc agggtccggt gtcccccatc tcccagaaat
     1921 tatccttcct gctgcccagc aggatgcgct ccggggcgat ccatttcttc cattcttcga
     1981 acgcctcgct gtcctgtggg agcccgcttt ccttgtcacc ttcgaatacg gtgacataca
     2041 gccggtcttt gggaatgccg tattccgcgg tcagcagctc ccagctccag gcgatggcct
     2101 ccttcttaaa ataccccgcg gcggcatcgg caggatcacc aaagctccag ttaccgagca
     2161 tttcgaacat ggtatggtga taggtgtcga cccccacctc ttccaggtcg ttgtgctttc
     2221 cgctgacccg aaggcatttc tgggtatcgg ctacccggga gtggggcgcc ttcttattgc
     2281 cgagaaaata atctttgaac tggttcatcc ccgcattggt gaat
//
LOCUS       DATGFG010000155         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_441292, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000155 DATGFG010000000
VERSION     DATGFG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441292"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VKQ52_02895"
     CDS             <1..238
                     /locus_tag="VKQ52_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86155.1"
                     /translation="ALQDKVSSRSAEAEEKSRRIQALSQEILELQQQISAMQQEISEN
                     KEKLMASSSGYTAECERRRQQIQDDIEKIKNYIP"
     gene            257..1249
                     /locus_tag="VKQ52_02900"
     CDS             257..1249
                     /locus_tag="VKQ52_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86156.1"
                     /translation="MNTLIQKTKTPGWWSEPKNIATAVVGLSLTAGLAYGFLTYVLPW
                     LVSVTWSIVNLVIGGVVCGFLLMLVSNRKFWRALKYLSEFLANYSIGLAIELNPFNIL
                     QAKIDQGYKDRDTLYKQSGRLKGKQSELMDKLSEKEKELQLNIQKVKILQSEGGSGRQ
                     VNLYTNNVMRCREYIDNVTPIVGDLNKLIQFADAAYEESGIMLEDAKLDLEAKKDLYY
                     SVTTGLSAVTSAMKAFKGDDELNRDAEKALALLKVRIGEKIGHIKSAIDVTSRFMDDQ
                     VLEDKAKSAQALELINRFDLGTDFHYTGTALKDGSDKGRLKDVNVTDRYKGLLD"
     gene            1282..3048
                     /locus_tag="VKQ52_02905"
     CDS             1282..3048
                     /locus_tag="VKQ52_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ86157.1"
                     /translation="MSVKLKPAGKLLIIAIVVTVGVLSVKWYQDRPKEAGKAVEVGKV
                     VLPDAPEASLNEKAVLLPVPSSTEAVNGGTRVVWERMAWNAQFSGMYANGGVRTTKGS
                     LFDKAHLDITYQRQDDCNKQMADLVKFAGEYKSNPNTPAILISFMGDGMPAFMTSLSK
                     SLESLGPEYQPIILPIAHGKSFGEDQVMGPASWKQDPHNAIGRCVAGVLRDGDVNILL
                     KWAGDNGIKVNPDETTYDAEAINLIAANDFLDAPNKYITGYTESRKVIVNGRTTGRDT
                     TVGVDAVATWTPGDVNVATKKGGLVTIANTHQYASQMPVQTIAIKKWAYDHRTDIEDM
                     IIALAQAGDQVRSFTEARKFASEVSAAVYQEQNGAYWLKYYNGVEEQDVQGLKVHLGG
                     SAVFNLADMANSYGLGADKVDRYKAVYNTFGNLMVKMYPTIMPNFIPYEKAVDKSFLL
                     SVISNHPELLEGKAIETRYASEITEAVSSRSYHIEFETGSAVIRPDSYKMLDEIFQSA
                     VVAEGLKIGVFGHTDNNGSDEVNVPLSEKRAAAVKEWLVRRGLKEDRIETRGFGSTKP
                     VADNATDAGRSRNRRVEIVLGE"
     gene            3096..>3542
                     /locus_tag="VKQ52_02910"
     CDS             3096..>3542
                     /locus_tag="VKQ52_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86158.1"
                     /translation="MFRPFALMSKTNILILIAAQVLFVLIVWQLSPGGLIPGPLRVLE
                     ALGQLLTTRLLFDNLFSSLLLTLRALVYSVVLTLLFSYLSVLPFFRPVAQFMVKCRYL
                     TLTGLIFIFTLLTRDGGALKLSLLVFGIVPFFMTSFLSVILRTPAQE"
BASE COUNT          807 a          875 c         1087 g          773 t
ORIGIN      
        1 cgccttgcag gacaaggtga gcagccgaag cgccgaagcc gaagaaaaga gccggcggat
       61 ccaggccttg tcgcaggaga tcctcgagtt gcagcagcag atcagcgcta tgcagcagga
      121 gatctcggag aacaaggaga aactgatggc cagcagcagc gggtacaccg cggaatgtga
      181 aaggcgccgg cagcagatcc aggacgatat cgaaaagatc aagaattata taccataacc
      241 tcaaaactaa atgaccatga acacgctcat tcaaaaaaca aaaacacccg gctggtggag
      301 cgaaccgaag aacatcgcta ctgctgtcgt tggcctctcg ctgacggccg ggttagccta
      361 tggatttctc acgtatgtgt taccctggct tgtatcggtg acctggagta tcgtcaacct
      421 cgtgatcggc ggtgtcgtct gcgggttcct gttgatgctt gtttccaacc ggaagttctg
      481 gcgggccctg aaatatcttt cggagtttct cgccaactat tccatcgggc tggccatcga
      541 gctgaaccct tttaacatcc tgcaggccaa gatcgaccag ggttataagg accgggacac
      601 tttgtacaag cagtcgggca ggctgaaggg caagcaaagc gaactgatgg ataagctctc
      661 cgagaaggag aaggagctgc agctgaacat ccagaaggtg aagatcctgc agagtgaagg
      721 cggttcgggt cgccaggtga acctctacac gaacaacgtt atgcgttgcc gggagtatat
      781 cgacaacgta acgccgattg tgggcgactt gaacaagctg atccagtttg ccgatgccgc
      841 ctatgaggaa agcgggatca tgctggagga tgccaagctg gatctcgagg cgaaaaagga
      901 cctgtattat tccgtgacca ccgggctttc cgccgtcacc agcgccatga aggcgttcaa
      961 gggggatgac gagctgaaca gggatgccga gaaggctctt gccctgctga aggtgcggat
     1021 cggggaaaag atcgggcata tcaaatcggc gatcgatgtg acttcccggt ttatggatga
     1081 ccaggtgttg gaagacaagg ccaagtccgc ccaggctttg gagctgatca accggttcga
     1141 cctcggcacg gacttccatt acacggggac ggcgctgaag gatggttccg acaagggccg
     1201 gctgaaggac gtcaacgtta cggatcgcta taaagggcta ttggattaaa cattatttat
     1261 taaaaattaa aacttactta tatgtcagtc aaattgaaac ccgccggtaa attattgatc
     1321 atcgccatcg tggtgacggt gggtgtcctt agtgtgaaat ggtatcagga caggccgaag
     1381 gaggccggca aggcagtgga agtggggaag gtggtattac ccgatgcccc cgaggcctct
     1441 ctgaatgaaa aggccgtgtt actgcccgta ccttcttcaa cggaggccgt caacggcggt
     1501 acccgggtcg tgtgggagcg gatggcctgg aacgcccagt tctcgggcat gtatgccaat
     1561 ggcggtgtac ggaccacgaa gggttctttg ttcgacaagg ctcacctgga tatcacttac
     1621 cagcggcagg acgactgtaa caagcagatg gccgacctgg tgaagtttgc cggggagtac
     1681 aagagcaacc cgaatacgcc ggctatattg atctccttta tgggtgacgg gatgcctgcc
     1741 tttatgacct cgttgtcgaa gagcctggag tcccttggac ccgagtatca gccgatcatc
     1801 ctgcccattg cccatggcaa gtcctttggt gaggaccagg tgatgggtcc cgcttcctgg
     1861 aaacaggacc cgcataatgc catcggcaga tgtgtagccg gtgtattgcg agacggcgac
     1921 gtcaatatcc tgttgaagtg ggccggggac aacgggatca aggtcaatcc cgatgagacc
     1981 acgtatgatg ccgaggccat caacctgatc gccgccaatg acttcctgga tgcgcccaac
     2041 aagtacatca cgggttatac ggaaagcagg aaggtcatcg tcaacggcag gacgacgggc
     2101 cgggatacga cggttggtgt ggatgcggtg gccacctgga cgccgggtga cgtgaatgtc
     2161 gcaacgaaga agggtgggct tgtaacgatc gccaatacgc accagtatgc atcgcagatg
     2221 cctgttcaga cgatcgcgat caagaaatgg gcttatgacc atcgtaccga tatcgaggac
     2281 atgatcatcg cgcttgccca ggcgggcgac caggtgcggt cctttaccga ggcgcggaaa
     2341 ttcgcttcgg aggtgagtgc ggcggtgtac caggagcaga atggcgccta ctggctgaaa
     2401 tattataatg gtgtcgagga gcaggacgtc caggggttga aggtgcatct tgggggttcc
     2461 gccgtcttca atcttgccga tatggccaac agctatgggc ttggcgcgga caaggtagac
     2521 cgttacaagg cggtgtataa cacttttggc aacctgatgg tgaagatgta tcccaccatc
     2581 atgcccaact tcatccccta tgaaaaagcc gtggacaagt ccttcctgct tagtgtgatc
     2641 tccaatcacc ccgagctgct ggaaggaaag gctatcgaga ccaggtatgc cagcgagatc
     2701 acggaggccg tttcctcgag gtcgtaccat atcgagttcg agacgggttc ggctgttatc
     2761 cggcctgact cttacaagat gctggatgag atcttccagt cggccgtggt tgccgagggt
     2821 ctgaagatcg gggtgttcgg tcacaccgac aacaatgggt cggatgaggt gaatgtgccg
     2881 ttgtcggaga aacgggccgc tgccgtgaag gagtggctgg tgcgccgcgg gctgaaggaa
     2941 gaccggatcg agaccagggg ttttggttcg acaaagcctg tagccgacaa tgcaacggac
     3001 gccggcagga gccgtaaccg tcgtgtggag atcgtgcttg gtgaataatt gtttaacaag
     3061 cgggggcgca ggcccccgct ttaaatctca gttgtatgtt tagacctttt gcgcttatga
     3121 gcaagacgaa tattctgatc cttatagccg cgcaggtgtt attcgtgctg atcgtctggc
     3181 agctgagtcc cggcgggctg atcccggggc cgctgcgggt gctggaagcg ctgggacagc
     3241 tgctgacgac gaggctttta ttcgacaacc tgttctcgag cctgttgctt acgctcaggg
     3301 cccttgtcta ttcggttgtg ctcacgcttt tattcagtta tttgtcggtg ctcccctttt
     3361 tccggccggt ggcgcagttc atggtcaaat gccggtatct gacgctgacg gggctgatct
     3421 ttattttcac cctgctgacg cgggatgggg gtgcgctgaa gttatccctg ctggtgtttg
     3481 gcatcgtgcc tttctttatg acctcctttt tgtcggtgat cctccggacg ccggcgcagg
     3541 ag
//
LOCUS       DATGFG010000156         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_770864, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000156 DATGFG010000000
VERSION     DATGFG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770864"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VKQ52_02915"
     CDS             <1..258
                     /locus_tag="VKQ52_02915"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HLZ86159.1"
                     /translation="HELGFSRNGDQYTAKSTLIVKGEDSLHTAMARTVGLPLGIAAIL
                     VLEGRINLRGLHIPILPVLYEPVMQELERQGIRFEHRITRN"
     gene            complement(314..1666)
                     /locus_tag="VKQ52_02920"
     CDS             complement(314..1666)
                     /locus_tag="VKQ52_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86160.1"
                     /translation="MTGYKRIWLVAGLLLSTFWAMAQDDLSAPVQQLRDYCKRELPTG
                     YSPRLNRLTLMPKSNLHYLFPLYHGFKDEEKFRKLYSDKGFYDEMSQYFAFAGDYRTA
                     LQYLVKSYDSVDESARRKVYKTAANLGDLEHADAAKYIHLAAKNRRVVMINEAFAKPL
                     HRAFTYSLLEDFYRMGFRYLAMEMLNNFSNQRLSSVGMQSGYYVCEPVAGELMRKAIE
                     LGYTLVPYEDTLASVHTADQRDSAQAAHLYEVVRKDSSARILVHASYAHISKKKGPDG
                     HIPMALAFWRLSGIEPLTIDQTDMTEDGNFGYGRIIYQAYTSRFKISGPSIALENNVP
                     VNVTDKDLYDLCVIHPPTQYLDGRPTWMSLGGTRQATYVKSPSNAVFLVQAYYQQEID
                     HNDNTPWQLVPADQAYTPGGRDGMGRSRYLLYLHKGKYKIFFRDINYHILSTLSIEVS
                     "
     gene            complement(1688..2215)
                     /locus_tag="VKQ52_02925"
     CDS             complement(1688..2215)
                     /locus_tag="VKQ52_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86161.1"
                     /translation="MNPRNDVSIASVYEPHYGLQLLLLLCFLIPAIFFLLTEQNTLRR
                     IKRENRLMHPGLVWLQIIPLFGQVWQFIVVSRIAGSIRNEMASWHNESIFGADEALVE
                     GGYGRPTLGIGIAYCTINVLLIIFNLSNRAHYVALDTLVGSLALAQIICWIIYWVQLA
                     RYKNKLKRGRLAGEG"
BASE COUNT          530 a          681 c          607 g          481 t
ORIGIN      
        1 cacgaactcg gcttttcccg taacggagac caatacacgg cgaaaagcac cctgatcgta
       61 aaaggcgagg acagcctcca taccgccatg gcccgcaccg tcggtcttcc cctcggcatc
      121 gcggccatcc tggtgctgga agggcgtatc aacctgcggg ggctccatat ccccatcctc
      181 cccgtcctct acgaacccgt catgcaggag ctcgaacgcc agggcatccg tttcgaacac
      241 cggatcaccc gaaactgacc ggtcccggga caccgacggc tcatgccggt atcttccgcc
      301 gcccaatccc cggtcagctc acttcgatac tgagggtgct caggatatgg taattgatat
      361 cccggaaaaa gatcttgtac ttccccttgt gcagatagag caggtaccgc gaccggccca
      421 ttccatcccg cccgcccggc gtataggcct ggtcggcagg caccagttgc cagggggtat
      481 tgtcgttatg gtcgatctcc tgctgataat acgcctgtac aaggaaaacg gcattcgacg
      541 gggattttac ataggttgcc tgtcgcgtcc ctccgaggct catccaggta ggccggccgt
      601 ccaggtactg ggtcggcgga tggatgacgc aaagatcata caggtcctta tcggtgacat
      661 tcaccggcac attattctcc agggcgatgg acggcccgct gatcttgaac ctcgaggtat
      721 acgcctggta aatgatccgg ccgtacccga aattaccgtc ttccgtcatg tcggtctggt
      781 cgatggtcag cggctcgatc cccgaaagcc gccaaaaggc cagcgccatc gggatatgcc
      841 cgtccgggcc ctttttcttg ctgatatggg cataagaagc atggacaagg atcctggccg
      901 aggaatcctt tcgcaccacc tcataaagat gggcggcctg cgccgaatcc cgctgatcgg
      961 ccgtatgcac ggaggccagc gtgtcctcat agggaacaag cgtatacccc agttcgattg
     1021 cttttcgcat caattccccc gccacgggct cgcagacata gtaccccgat tgcatcccga
     1081 cgctggacag ccgctgattg gaaaaattgt tcagcatctc cattgccagg tagcggaagc
     1141 ccatccggta aaaatcctcg agcagggaat acgtaaaggc gcggtgcagg ggcttggcaa
     1201 aggcctcgtt gatcatcacc acccggcggt tcttcgcggc gaggtggata tacttggcgg
     1261 catcggcatg ttcgagatcg cccaggttgg ccgccgtctt gtagaccttc cggcgggcgc
     1321 tctcgtcgac actgtcgtag ctcttcacga ggtattgcaa cgcggtgcga taatccccgg
     1381 caaacgcaaa atactggctc atctcgtcat agaagccctt gtcggaatag agcttccgga
     1441 atttctcttc gtccttgaac ccatggtaga ggggaaacaa atagtgcagg ttgctcttgg
     1501 gcatcagggt cagcctgttg agccgcggac tgtaccccgt ggggagttcc ctcttgcaat
     1561 agtcccgtag ttgttgaaca ggggcggaaa gatcatcctg ggccattgcc caaaaagtgg
     1621 aaagaagcaa ccccgccacc agccaaattc gcttatatcc ggtcatttcg gtgaaaatat
     1681 cactttttta cccctcgccc gccagccgtc cccgctttag cttattttta taacgcgcca
     1741 gctgcaccca atagatgatc cagcagatta tctgggccag cgcaagagaa cccacaagcg
     1801 tatcgagggc aacataatgg gcgcgattgc tcagattgaa aataatgagg aggacgttga
     1861 tcgtacaata agcaatgccg atccccagtg tcggtcgccc gtaaccccct tccacaagag
     1921 cctcatccgc cccgaaaatg gactcattat gccaggaagc catttcattc cggatcgaac
     1981 cggcgatcct ggagacgaca ataaactgcc acacctgccc aaacaaaggg atgatctgca
     2041 accatacaag ccccggatgc atcaggcggt tctcccgctt tatccttctc agggtgtttt
     2101 gctcggtaag cagaaagaag atagccggga taagaaaaca caaaagaagg agcaattgga
     2161 gcccatagtg cggctcatag acggacgcga tggaaacatc gttgcgcggg ttcattgacg
     2221 gggatttagt cctctaaata tagggaacag gcgccagccc tcctataagg ccgccagcat
     2281 tttctgcagc tccttcaca
//
LOCUS       DATGFG010000157         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1572705, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000157 DATGFG010000000
VERSION     DATGFG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572705"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VKQ52_02930"
     CDS             <1..381
                     /locus_tag="VKQ52_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86162.1"
                     /translation="YILSYWSRDGGVNLSGVTLVGTRTGPTKNGWTYYEHTITAVVGG
                     SVTLGASSAKTIDELRLYPVSAQMTTFTHTPLIGITSRCSENNTVTYFNYDAFDRLRF
                     IRDADGNIVKTMDYHYQQPGNITY"
     gene            436..>2753
                     /locus_tag="VKQ52_02935"
     CDS             436..>2753
                     /locus_tag="VKQ52_02935"
                     /inference="COORDINATES: protein motif:HMM:NF039454.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6443 domain-containing protein"
                     /protein_id="HLZ86163.1"
                     /translation="MQAIKTRWPGHQWTALVLALFFSVTGSAQVSISGTNCVVAGGTI
                     GYQYTISGAWQQTDNVTWTIVGGVIAGTNNTSKSGTVSTIGPSISVTWNPGATGAQVQ
                     LYESRLGSNTMNVTVTTFTNTLTPASQAVSYGSTVTISGGGAGTGCSPSYGYSWESAS
                     ASTGPFSPINGAAGQDLTINSFTQPAYYRRALSFNADVVYSNTVSVTIAPLAAGSISA
                     SNTSPGYNTQASITETPASGGYCSSLTYEWQQSVEGGPWQPIGSGEAYPSGAPALVGN
                     TLIRRKVMCSNQVLFTNTLPFTVNYSAASAEPYNYIRVSDVAIPGISSFEQMDQLAIG
                     QKAQTTQYFDGLGKPIETVARQASPLQKDLVTPVRYDNFGREVLKYLPYVSTAGDGLY
                     KINPLAEQNSFNSAQFPGEQYYYAQTGFENSPLDRVDTAYAPGLSWVGSSRGVVTQHL
                     VNTLSGDSVRIWKISLSSDSLPYSAGIYGDGLLYKTVTADEQGHQVVEYKDREGKVIL
                     KKVQLADNPGTAHVGWLCTYYIYDDLNNLRFVLPPRATELINGPWVINPSIAAELCFR
                     YVYDARNRMVIKHVPGAGDVWMIHDVRDRLVMTQDSNLRRGQQWLVTQYDGLNRPVET
                     GLITYAATRSLLQQQVTSQTAGGGQTAPNEPADTTVATADMTGDLRASRSITADSGFS
                     TLDNGEFTAEIVNGDWGSGGSITNSNVVSNSPVPPGVTLQPLTFTYYDDYAWVAGSNT
                     ALPAAMATGIGSNQNYFVTTYNTGPVYAVPVTP"
BASE COUNT          603 a          816 c          784 g          550 t
ORIGIN      
        1 tatatcctgt cctactggtc ccgggacggc ggcgtcaacc tgagcggcgt gaccctggtc
       61 ggtacccgga ccggccccac gaaaaacggc tggacgtatt atgaacatac gatcaccgcg
      121 gtggttgggg gcagcgttac cttgggcgcc tcctccgcca agacgatcga tgaattacgg
      181 ctctaccctg tctccgccca gatgacgacg ttcacccata ccccgctgat cggcatcacg
      241 agccggtgca gcgagaacaa tacggtaacg tatttcaact acgatgcttt cgatcggctc
      301 cggttcatca gggatgccga tgggaatatt gtcaaaacca tggactacca ttatcaacag
      361 cccggaaaca taacctatta agcgggacag ctgaatgtcc cgacgagtaa cgatggcccc
      421 tttaaccgtt cgtttatgca agcaataaaa actagatggc ctggtcacca gtggacggcg
      481 ctcgtcctgg cgctcttttt ttcggtaaca gggtccgccc aggtgtcgat cagcggaacc
      541 aactgcgtcg tagcgggggg gacgatcgga tatcaatata ccatctccgg cgcttggcaa
      601 cagaccgaca atgtcacctg gaccattgtc ggaggcgtga tcgcgggtac caacaacacc
      661 agcaagtcag ggaccgtgag taccatcggg ccctccatca gcgtcacctg gaacccaggc
      721 gcgacaggcg cccaggtgca actctatgaa agccggctcg gcagcaacac catgaatgta
      781 acggtgacta cgtttaccaa taccctaacg ccggccagcc aggccgtgag ttatggttcc
      841 acggtaacga tatcgggcgg cggggcaggc acgggttgtt ctccttcata tgggtattcg
      901 tgggaaagcg cttccgccag taccggtcct tttagcccga tcaacggcgc cgccgggcag
      961 gatctgacga tcaattcctt tacccagccg gcgtattacc ggcgggcgct atcgttcaat
     1021 gccgacgtcg tgtatagcaa tacggtctcg gtgacgatcg caccgctggc cgcaggcagc
     1081 atttccgcct cgaatacttc tcccggctat aatacgcagg cctccattac cgaaacgccg
     1141 gcatccggcg gctattgcag cagcctcacc tatgagtggc agcaatcggt ggagggtggc
     1201 ccctggcagc ccatcggttc aggcgaagcc tatccgtcgg gcgcccccgc cctggtgggc
     1261 aacaccctca tccgaagaaa ggtgatgtgt tccaaccagg tgcttttcac caataccttg
     1321 ccctttacgg tgaattattc cgctgcaagc gccgaaccct acaactacat ccgcgtcagc
     1381 gacgtggcga tacccgggat cagtagtttc gaacagatgg atcagctggc cattggccag
     1441 aaggcgcaga cgacgcaata tttcgacggg ttgggtaagc ccattgaaac ggtggcccgt
     1501 caggcctctc ccttgcagaa agacctggta acgcctgtac ggtatgataa tttcggccgg
     1561 gaagtgttga agtacctgcc ctatgtttcc accgccggtg acgggctgta taaaattaac
     1621 ccgctggccg agcaaaacag cttcaacagc gcgcagtttc ccggcgagca atactattat
     1681 gcccaaacgg ggtttgagaa ctccccattg gaccgggtcg acacggccta tgctcccggc
     1741 cttagttggg taggatcttc caggggcgtc gtcacccaac acctcgtcaa taccctgtcg
     1801 ggcgatagcg tgcggatctg gaagatcagt ctttcctcgg acagcctgcc ctatagcgcc
     1861 gggatctatg gagacggcct gctctacaag actgttaccg cagatgaaca ggggcaccag
     1921 gtggtcgagt acaaggacag ggaggggaag gtgatcctga agaaggtaca gttggccgac
     1981 aatcccggta cggctcacgt ggggtggctt tgtacgtatt atatttatga tgacctgaat
     2041 aatttacgtt ttgtcctgcc accccgggcc acggaattga tcaacggacc ctgggtgatc
     2101 aacccgtcca tagctgctga gctctgtttc cgctatgtct atgacgcccg aaaccggatg
     2161 gtcataaagc atgttcccgg tgcgggggat gtctggatga tccatgatgt ccgcgaccgg
     2221 ctggtgatga cccaggattc caacctccgg cgagggcagc aatggctggt gacccaatac
     2281 gacgggctga accggcccgt ggaaacggga ttgatcacct atgccgccac acggtctctc
     2341 ctgcaacagc aggtcaccag ccaaacggcc ggcggcggcc agacggcgcc gaacgaaccc
     2401 gcagacacga ccgtcgccac ggcggacatg acgggcgatc tcagggcctc ccggtcgatc
     2461 actgccgaca gcgggttttc cacgctggac aatggcgagt tcacggccga gatcgtcaat
     2521 ggcgactggg gcagcggcgg gtcgattacc aacagcaatg tggtaagcaa cagcccggtg
     2581 ccgcctggag taacgctgca acccctgacc ttcacttact atgatgacta tgcctgggtg
     2641 gccggctcca acacggcatt gccggccgcg atggctacgg gcattggcag caatcagaat
     2701 tattttgtca cgacgtacaa tacgggtccc gtctatgcgg tgccggtcac tcc
//
LOCUS       DATGFG010000158         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_770371, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000158 DATGFG010000000
VERSION     DATGFG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770371"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VKQ52_02940"
     CDS             <1..618
                     /locus_tag="VKQ52_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HLZ86164.1"
                     /translation="AEGKGGIYNFVTKRGDCRGARSKISWTQVETGSAITWKYPSVIL
                     QGDNSTGEFYSVAVTRNRQIADTGTKMHHIGRNTRSRIISKGISAGQGQNSYRGLVQI
                     GAKADEARNFTQCDSLLIGDQCGAHTFPYIESKNNTATVEHEATTSKIGEDQIFYLNQ
                     RGIDSEKAVALIINGYAREVLNQLPMEFAVEAQKLLSITLEGSVG"
     gene            633..1403
                     /gene="sufC"
                     /locus_tag="VKQ52_02945"
     CDS             633..1403
                     /gene="sufC"
                     /locus_tag="VKQ52_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HLZ86165.1"
                     /translation="MLIIKNLHAEVEDKKILKGIDLTVNAGEVHAIMGPNGSGKSSLA
                     SVLAGRESYQVTEGEVLFEGKNLLEMDPETRAREGLFLAFQYPIEIPGVSNINFLKTA
                     IGEIRAYKGMPPLDAKEFLRMSREKQKLVEFDAGLVNRSLNEGFSGGEKKRNEIFQLA
                     MLEPRLSILDETDSGLDIDALRIVSQGVNKLRSEKNAFIIITHYQRLLEYIVPDFVHV
                     LYNGRIVKSGTKELALELEEKGYDWLKEESREKEATTI"
     gene            1409..>2372
                     /locus_tag="VKQ52_02950"
     CDS             1409..>2372
                     /locus_tag="VKQ52_02950"
                     /inference="COORDINATES: protein motif:HMM:NF040245.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HLZ86166.1"
                     /translation="MNALQEQTIPLYGRIVEKYNQLSAAGAPQAARQSAFHRFQLLGF
                     PTIRNEDWKYTNITRFLKDEYALESSVTVPPAGLLAKATIPGLECYGLVLVNGSWQGE
                     ITGGPLPKGITVHRVADALRQPGFADFFAAENWGNQHFAALNAALYTDGLFIHAEANA
                     AIDKPLHIIHVYASDHNLLVQPRHVWVLNRNAELSIIESTVTTSGEAKIWINSLTDII
                     AGQDAKLDHVNLQTAAEGARLIQQTNVHQKRQSLYNNYTFTLPGADLVRNDLQARLEE
                     DHTETHFYGLYLTAGNQLVDNHTLVDHQKPHCFSNELYKGVLLEQ"
BASE COUNT          612 a          753 c          590 g          417 t
ORIGIN      
        1 gccgagggca agggcggcat ctacaacttc gtcaccaagc gcggcgattg ccgcggcgcg
       61 cgcagcaaga tctcctggac ccaggtcgag accggctccg ccattacctg gaaatacccc
      121 agtgtcatcc tgcagggtga caactccacc ggggaattct atagtgtagc cgtcacccgc
      181 aacaggcaga tcgccgacac gggaaccaag atgcaccata tcggccgcaa cacccgcagc
      241 cggatcatct ccaagggcat ctccgccggt cagggccaga acagctacag aggactggta
      301 cagatcggtg caaaggccga cgaagcccgg aatttcaccc agtgcgactc cctgctcatc
      361 ggcgaccagt gcggcgcgca caccttcccc tatatcgagt cgaagaacaa caccgccacg
      421 gtggaacatg aagccaccac ctccaagatc ggcgaagacc agatcttcta tctcaaccag
      481 cggggcatcg attcggagaa ggccgtcgcc ctcatcatca acggctatgc ccgggaagtc
      541 ctcaaccagc tccccatgga gttcgccgtc gaagcgcaaa aattattatc tattacgctt
      601 gaaggaagtg taggttaaac aacacaatat aaatgctgat cattaaaaat ttacacgccg
      661 aagtcgaaga caagaagata ctcaagggca tcgaccttac cgtcaatgcc ggcgaggtac
      721 acgccatcat gggaccaaat ggctccggca aaagctccct ggcctcggta ctggccggca
      781 gggagagcta ccaggtaacg gaaggagaag tactgttcga aggaaagaac ctgctggaaa
      841 tggaccccga gacccgggcg cgcgaaggcc tctttctagc ctttcagtac cccatcgaga
      901 tccccggcgt ctccaatata aacttcctga agaccgccat cggcgagatc cgcgcctata
      961 agggcatgcc cccgctcgat gcaaaagagt tcctgagaat gtccagggaa aaacagaaac
     1021 tggtcgaatt cgatgcggga ctggtcaacc gcagcctcaa cgaaggcttc tccggcggcg
     1081 agaaaaaacg gaacgagatc ttccagttgg ccatgctgga accccggctc tccatcctcg
     1141 acgaaacgga ctccggcctc gacatcgacg ccctgcgcat cgtttcccag ggggtgaaca
     1201 agctgcgttc cgaaaagaat gcctttatca tcatcaccca ctatcagcga ttgctggaat
     1261 atattgttcc cgacttcgtc cacgtcctct acaatggccg gatcgtaaag tccggtacaa
     1321 aagaactggc gctcgaactg gaagagaaag gatacgactg gctaaaggaa gagtcccgcg
     1381 aaaaagaagc aactactatt tgaacaccat gaacgcgcta caagaacaga cgatccccct
     1441 ctacgggcga atagtcgaaa aatataatca attgtcggcc gcaggcgcac cccaggcggc
     1501 aagacagtcg gcctttcatc ggttccagct gctgggcttc ccgacgatca ggaacgaaga
     1561 ctggaaatac accaatataa cccgttttct gaaggacgaa tacgccctcg aatcttcggt
     1621 aaccgttcct cccgccggcc tcctggcaaa agcgacgatc cccggcctcg aatgctatgg
     1681 tctggtcctc gtcaatggct cctggcaggg agagatcacc ggtggcccgc tcccgaaggg
     1741 catcacggtc catcgcgtcg ccgacgccct ccggcaaccc ggcttcgccg acttctttgc
     1801 cgccgagaac tggggtaacc agcacttcgc cgccctgaac gccgccctct ataccgacgg
     1861 cctcttcatc catgccgaag caaatgcagc tatcgacaaa cccctgcata tcattcacgt
     1921 gtatgccagc gatcacaacc tgctcgtcca gccccgtcat gtctgggtgc tcaaccggaa
     1981 tgccgaactc tccatcatcg agtccacggt cacgacctcc ggcgaggcaa agatctggat
     2041 caacagcctg accgatatca tcgcgggtca ggatgctaag ttggaccatg tcaacctgca
     2101 aaccgccgcc gaaggcgcac gcctcatcca gcagacaaac gtccaccaga agaggcaaag
     2161 cctgtataac aactatacct tcaccctgcc cggcgccgat ctcgtccgca acgatcttca
     2221 ggcccggctc gaggaagacc ataccgagac ccatttctat ggcctctacc tcaccgcggg
     2281 caatcaactg gtcgataacc atacgctcgt cgatcaccag aaaccgcatt gcttcagcaa
     2341 cgaactctac aagggcgtat tgctcgagca at
//
LOCUS       DATGFG010000159         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_545346, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000159 DATGFG010000000
VERSION     DATGFG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545346"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1310)
                     /locus_tag="VKQ52_02955"
     CDS             complement(231..1310)
                     /locus_tag="VKQ52_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HLZ86167.1"
                     /translation="MQILTEPIGSIPRPLELVEGLKAFSSGDIQAAELEILFERALKD
                     TLQRFEASGSPVITDGEQTKPSFATYPLAGSTQLDPNGIVIPIADGHTRQLPKLNGGP
                     FHYQVYAGDFYKRAALMTRLPVKQAVISGSALSLLYPAEGLVDYGREAFLNDLYAEHE
                     KDIRSGLDAGAHTVQIDFTEARLALKLDPSRALLRSFIDLNNTVLNRLTPAERQRVGV
                     HSCPGGDHDSTHSADIPYSELLPLLFELNVTNFYLQYAGEYNKRQVLEVIKENLRPGT
                     RVFIGVTDVLSPRIETPQEICDLILEAVQYIPPAQLGTTDDCGFSPFADDTSTSRDIA
                     FEKIRARIEGTRMAEKALGKMQIAL"
     gene            complement(1336..2427)
                     /locus_tag="VKQ52_02960"
     CDS             complement(1336..2427)
                     /locus_tag="VKQ52_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86168.1"
                     /translation="MRKYFLSLVFLTVFASTNELFSQGCVAIRSTGGFCSYNGVRHID
                     SASKWQFTVNNRYYKSFRHYVGSVEQKQRQTLGNAVINSAYTMDMAIYRIINPRWSLM
                     LDMPISANNRSQAFLEKGLYNRYSTNAFGVGDIRVAVYSWLLDPVKMPKGNVQVGLGL
                     KFPTGNDNVQDWFKTSDSTKTFGPVDQSIQLGDGGTGITLEVNAYYNFSHTVGVYGSF
                     FYLSNPADQNGISNAHGGTPSATSVANTSSVMSVPDQYLIRAGVNAQFNRLTASAGFR
                     DDCLPVHDLIGKSDGFRRPGYILSVEPGLTYVFSSKVSVYSYVPIACVRNRTQSVPDK
                     ITTALTGVYAHGDAAFADYVINIGANFRF"
     gene            complement(2408..3277)
                     /locus_tag="VKQ52_02965"
     CDS             complement(2408..3277)
                     /locus_tag="VKQ52_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86169.1"
                     /translation="MTDLSGSGPHSPAQKIHYTLRIASAMCFIGHGAFGIITKPIWCN
                     YFAVAGIGHDLAYRLMPLVGVADILCGLCLLVYPLRAVAAWLVCWGLITASMRPLSGE
                     PFAEMIERAGNYCAPLALLLLSGISGGREKKVGLPGKAIGGGLLSRLDPHSRPDERGF
                     RMVLLTLRIAVFLLLLGHGWLNLIEKKSLLQQYTALGFSQPVQVARLVGMFELLAAVA
                     VLVRPVRPLLLAFFVWKMATELFYPQWEAFEWIERGGSYGCLLALWFALEKTPINQLT
                     FTQNTAKNEKVFS"
     gene            complement(3363..>3703)
                     /locus_tag="VKQ52_02970"
     CDS             complement(3363..>3703)
                     /locus_tag="VKQ52_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86170.1"
                     /translation="GGVRYQYVKRRVFKDSLELLCIPNETALRLQQVKNDFFRQVNDL
                     QQQNQGKKSSTPIKDLSKDYQPTAMQVAVPDALAALAPVLSGVYTFPYLPSCYSPTAE
                     IPPDLAPALA"
BASE COUNT          823 a          994 c         1073 g          813 t
ORIGIN      
        1 cagctgctgt gcatataggt ggcaccgata caaagattgt tcttgtccat catgatggcg
       61 tcctcgtccg tttgagcctt cagcatcgcc ggctggaaca gggagaacag caggacggcg
      121 gccaggacga cttttctaca attcataagc cttgatttgg ttttataagt gttaagcatt
      181 ttttgcgtgt ccggatcgtt tattattggg ctattattat tgggctattg ttacagggct
      241 atctgcattt tcccgagtgc cttctccgcc attcgggtgc cctcgatcct cgcccttatc
      301 ttttcaaaag caatatcccg gctggtggaa gtatcatcgg cgaaggggga aaaaccacaa
      361 tcatccgttg tccccagctg cgccggcggg atatactgca ccgcttcgag aataagatca
      421 cagatctcct gcggcgtctc gatccgcggg ctaagcacat ccgttacccc gataaagacc
      481 cgtgtacccg ggcgcagatt ttcctttatc acctccagca cctggcgttt gttgtattcc
      541 ccggcatatt gcaggtaaaa attcgtcaca ttcagttcga ataacagcgg cagcaattca
      601 ctataaggga tatcggcgct atgggtcgaa tcatgatcgc cgccggggca gctgtgcacc
      661 cccacccgtt gccgttcggc cggcgtaagc cggttcagga cagtattgtt gagatcgata
      721 aagctgcgca acaatgccct gctgggatcc agcttcaatg ccagccgcgc ctcggtaaaa
      781 tcgatttgta ccgtatgtgc ccccgcgtca aggcccgagc ggatatcctt ctcatgttct
      841 gcatacagat cgttgagaaa agcttcccgc ccgtagtcca ccagcccttc cgcgggatat
      901 aacaggctaa gcgccgaacc cgagatcacc gcctgtttca ccggaagccg ggtcatcagg
      961 gcagcccgct tgtagaaatc accggcatac acctggtaat ggaatgggcc tccgttcagt
     1021 tttggcagtt gccgcgtgtg cccgtcggcg atggggatga cgatcccgtt ggggtcgagt
     1081 tgtgtcgatc cggcgagcgg ataggtggcg aaactcggtt tcgtctgctc cccgtcggtg
     1141 atgacagggg agccggaggc ctcgaaacgc tgcagcgtat ccttcagggc acgctcgaac
     1201 aggatctcca attccgccgc ctggatatcg cccgacgaaa atgcttttaa cccctctacc
     1261 agttcaagcg ggcggggaat actgcctatc ggttctgtaa gaatttgcat ggccgtaatt
     1321 tttttgttga gcagatcaaa aacggaaatt cgcgccgatg ttgatgacat aatcggcaaa
     1381 ggccgcatcg ccatgcgcgt agacaccggt cagggccgtc gtgatcttgt ccggcacact
     1441 ttgggtgcgg ttgcgcacac aggcgatggg gacataggag tagacggaca ccttggatga
     1501 gaatacatag gtcagccccg gctcgacgga aaggatatac cccgggcggc ggaagccgtc
     1561 gctcttgccg atgagatcat gcaccggcag gcagtcgtcg cgaaacccgg cggaagcggt
     1621 gaggcggttg aactgcgcgt tgacgcctgc gcggatcagg tattggtccg gaacgctcat
     1681 gacgctgctg gtattggcaa cagaggtggc ggatggcgtc ccgccatggg cgttggagat
     1741 cccattctga tcggcgggat tcgagagata gaagaagcta ccatagacgc ccacggtatg
     1801 ggagaaattg tagtaggcat tcacctccag cgtgatcccc gtgccgccat cacccagttg
     1861 gatggactgg tctacgggtc cgaaggtctt ggtgctgtcg ctggtcttga accagtcctg
     1921 gacattgtcg ttgccggtgg gaaactttaa tcccaatcct acctgcacat tgcccttcgg
     1981 cattttgacg gggtcgagca gccagctgta gacggcgacg cggatatccc ccaccccgaa
     2041 agcgtttgtg ctgtagcggt tgtacaggcc tttctcaagg aaggcctgcg agcggttatt
     2101 ggcggagatg ggcatgtcca gcatgagcga ccagcggggg ttgatgatcc ggtagatcgc
     2161 catatccatc gtgtaggcac tgttgatcac cgcattcccc agcgtctggc gttgcttctg
     2221 ctccaccgag cctacatagt ggcggaaaga tttataatag cggttgttga cagtgaattg
     2281 ccatttgctg gcggaatcga tatgccgcac gccattgtag ctgcaaaatc cgccggtgct
     2341 gcggatggcg acacatccct gtgaaaaaag ctcattagtg ctagcgaaaa cagttagaaa
     2401 gacgagacta agaaaatact ttctcatttt ttgcagtgtt ttgggtaaaa gttagttggt
     2461 tgatgggtgt tttttcaagg gcaaaccata acgcgagcaa acagccatag ctgccgccgc
     2521 gttctatcca ttcgaatgct tcccactgcg gatagaatag ttcggtggcc atcttccata
     2581 caaagaaggc gagcaacagg ggacgcacgg gccgtaccag gaccgcgacg gccgcaagga
     2641 gttcgaacat ccccacgagg cgggccacct gcacgggttg ggaaaagcca agggcggtat
     2701 attgttggag caggcttttc ttttcgataa ggttgagcca gccatgtccc aacagcagga
     2761 gaaagacagc tatgcgtaaa gtcaggagca ccatccggaa accgcgctca tccggccggg
     2821 aatgcgggtc aagccgcgac aacaggccgc cgccgatggc cttcccggga agccccacct
     2881 tcttctcccg accgccgctt atacccgaca ggagcagcag cgccaggggt gcgcagtagt
     2941 tgcccgccct ttcgatcatt tctgcaaagg gctcacccga caggggccgc atcgaagccg
     3001 ttataagccc ccagcaaacc agccaggccg ccaccgcccg gagggggtag accaacaggc
     3061 aaagaccgca caggatgtcg gccactccta ccagcggcat aagccggtag gcgagatcat
     3121 ggccgatacc ggcgacggca aaatagttgc accagatggg tttggtgatg atgccaaacg
     3181 cgccgtgacc gataaagcac atggccgaag caatgcgcag cgtataatgt attttttgtg
     3241 cgggggagtg gggcccgcta ccggaaagat ccgtcattgt atagctttat cttaccacca
     3301 cgaggccgat tctgttcata aaccggtcgt gaaaggttct ctgggaagaa cgattgactc
     3361 ttttaggcga gtgccggagc caggtcgggt ggtatctccg cggtgggtga ataacaggaa
     3421 ggaaggtaag gaaaggtata aacccctgac aacaccggcg cgagtgcggc cagcgcatcg
     3481 ggaacggcaa cctgcatcgc cgttggctga taatcttttg agagatcctt gatgggtgtg
     3541 gaggacttct ttccctggtt ttgctgctga agatcgttca cctggcggaa gaagtcattc
     3601 ttcacctgct gcagcctcaa cgcggtctcg ttggggatac aaagcagctc gagggagtct
     3661 ttgaataccc ggcgtttgac atattgatag cggacgccgc cga
//
LOCUS       DATGFG010000160         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1000111, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000160 DATGFG010000000
VERSION     DATGFG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000111"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VKQ52_02975"
     CDS             complement(<1..517)
                     /locus_tag="VKQ52_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86171.1"
                     /translation="MAKLNFGGVEENVVTREEFPLAKAQQVLKNEVVAVIGYGVQGPG
                     QALNQKDNGINVIVGQRKNSKTWDKAVRDGFVPGQTLFEIEEALQRGTIICYLLSDAA
                     QIALWPTVKKHLTPGKALYFSHGFGITFNEQTGIIPPKDVDVFLVAPKGSGTSLRRMF
                     LQGRGLNSSYAI"
     gene            complement(598..1188)
                     /gene="ilvN"
                     /locus_tag="VKQ52_02980"
     CDS             complement(598..1188)
                     /gene="ilvN"
                     /locus_tag="VKQ52_02980"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HLZ86172.1"
                     /translation="MNKQEYTITVYTENQIGLINRIAILFSRRKINIESLNTSPSEVE
                     GIHRFTIVINETEEVVRKLCRQLEKQVEILKAYYNTNEEIIWQELALYKVPTDEIAEK
                     VKVERLLREYGARAVVIRKDYTVFETTGQREEIDRLITALEPFGLIEFVRSARVAIIK
                     DSAGFHKKLKEFERTEPGEDVVENQYLNQQDKVFSM"
     gene            complement(1191..1967)
                     /locus_tag="VKQ52_02985"
     CDS             complement(1191..1967)
                     /locus_tag="VKQ52_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLZ86173.1"
                     /translation="MAALKYDTHWNANLYNDKHNFVFKYGEDVVQLLAPGKGERILDV
                     GCGTGYLTNLIAQAGARVVGIDNSPSMVQRAQAVYPDLDFRVLSATEFFFETPFDAVF
                     SNATLHWVLDKETAIDRIAQALRPGGRLVLEMGGKGNVEEIVVATRKVLTRHGYYSNA
                     ATQLWYFPSLSEYTTLLEKKGFRVGFAAHFDRLTVLKDTDNGIKDWIKMFGNAFFADI
                     PDSQRETVLEEIQEAVRPTNYRNGNWYGNYKRLRIAAVKL"
     gene            complement(1972..>3588)
                     /gene="ilvB"
                     /locus_tag="VKQ52_02990"
     CDS             complement(1972..>3588)
                     /gene="ilvB"
                     /locus_tag="VKQ52_02990"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HLZ86174.1"
                     /translation="PGGAIMPIYDALYDYRDRLNHILVRHEQGGIHAAQGYARTSGRT
                     GVVFATSGPGATNLVTGLADAMIDSTPVVCITGQVFAHLLGTDAFQETDVINITTPVT
                     KWNYQVTDATEIPAALAKAFYIAGSGRPGPVLIDITKNAQIQKFNYEGYTKCEHIRSY
                     RPKPIVRKEYIAKAAALINEAKRPFILFGQGVILGKAEQEFRAFVEKSGIPAAWTLLG
                     LSALPTDHPLNVGMLGMHGNYGPNLLTNDCDVLIAIGMRFDDRVTGRLDKYAKQARVV
                     HLDIDPAEIDKNVKATVPVWGDCKETLPLLTELVERRTFPEWHQRFKDCYQQEVEAVI
                     HDELNPTTAEMTMGEVMKVLNELTGGDAVIVTDVGQHQMVASRYAHFNQSKSNVTSGG
                     LGTMGFGLPAAIGAKYGAPDRTVVAIIGDGGFQMTLQELGTIMQFGAEVKILILNNRF
                     LGMVRQWQELFNDRRYSSVDITSPDFVALAKAYHIESSSINHRKELKTALQTMLDHKG
                     AYLLEVFTGKENNVFPMVPQGCSVSEIRLK"
BASE COUNT          603 a          854 c         1198 g          933 t
ORIGIN      
        1 agatggcgta gctgctgttg aggccgcggc cctggaggaa catccggcgg agggaggtgc
       61 cggagccttt tggtgcaacg aggaatacgt ctacgtcctt gggaggaatg atgcccgttt
      121 gctcgttgaa cgtgatgccg aagccgtggg agaaatagag ggctttgccg ggggtgaggt
      181 gttttttcac ggtgggccac agggcaatct gggcggcgtc gctcagcaga tagcagatga
      241 tggtgccgcg ttggagggct tcttcgatct cgaacaaagt ctggccgggt acgaagccat
      301 cgcgaacggc tttgtcccag gttttggagt tcttgcgttg acccactatg acgttgatgc
      361 cgttatcttt ctgattcagc gcctggccgg gaccctgtac gccatagccg atgacggcga
      421 ccacttcatt tttcaacacc tgctgtgctt tggcgagcgg gaattcttcg cgggtgacta
      481 cattctcttc cacgccgccg aaatttagtt ttgccattgt tacctgtttt atttgatttt
      541 ataagtgacg attttatccg aagcctttgg ccgttatggg ggccggcggc tgccggttta
      601 catactgaat accttgtcct gctggttgag gtattggttt tccactacat cttcgccggg
      661 ttcggtgcgt tcgaattcct ttagtttttt gtggaagcct gcgctgtcct tgatgatggc
      721 gacgcgggcg ctgcgtacga attcgatgag gccgaagggt tccagagcgg taatgagcct
      781 atcgatctcc tcccgctggc cggtggtctc gaacacggta taatctttac ggatgactac
      841 ggcgcgggcg ccgtattcgc ggaggaggcg ttcgaccttt accttttcgg cgatctcgtc
      901 ggttggaacc ttgtagaggg ccagttcctg ccagatgatc tcttcgttcg tattataata
      961 cgctttcagg atctctacct gtttttccag ttggcggcag agtttgcgga ccacttcttc
     1021 ggtttcgttg ataacgatgg tgaagcggtg gatgccctcc acttccgagg gagatgtatt
     1081 gaggctttcg atatttatct tccggcggga gaacaggatg gcgatgcggt tgatgaggcc
     1141 gatctggttt tccgtataga cggtgatggt atattcttgc ttgttcatgt ttatagtttt
     1201 actgctgcga tgcgtaatct tttataatta ccataccaat tcccgttgcg gtagttggtt
     1261 gggcggacgg cctcctggat ctcttcgagg acggtttccc tctgcgagtc ggggatatcg
     1321 gcgaaaaagg cattaccgaa catttttatc cagtctttga tgccgttgtc ggtatccttt
     1381 agtacggtga ggcgatcgaa gtgggcggcg aagccgacgc gaaagccttt tttctccagc
     1441 agggtggtat actcgctgag tgatgggaaa taccagagct gggtggcggc gttgctatag
     1501 tagccgtggc gggtgagcac tttccgggtg gccacgacga tctcttccac gttgcctttg
     1561 ccgcccattt cgaggacgag gcggccgccg gggcggaggg cctgggcgat gcggtcgatg
     1621 gcggtttcct tatcgagtac ccagtggagg gtggcgttgg agaagaccgc gtcgaagggg
     1681 gtttcgaaga agaattcggt ggccgacaat accctgaagt ccagatcggg atagacggcc
     1741 tgggcccgtt ggaccatgga tggggaattg tcgatgccga ccacccgtgc gccggcctgg
     1801 gcgatgaggt tggtaaggta acctgttccg cagccgacgt cgaggatgcg ttcccctttg
     1861 ccgggggcga gaagctggac gacatcctcc ccgtatttga agacaaagtt gtgtttgtcg
     1921 ttgtacaggt tggcgttcca gtgtgtatcg tattttagag ctgccattgt attattttaa
     1981 tctgatctcg cttacgctgc aaccctgggg gaccatcggg aagacgttgt tctctttccc
     2041 ggtgaagacc tcgaggaggt aggctccctt gtggtcgagc atggtttgca gggcggtttt
     2101 tagctctttc cggtggttga tgctactgct ttcgatgtgg taggcttttg ccagggcgac
     2161 gaagtcgggg ctggtgatgt ccacggagga atagcgcctg tcgttgaaga gttcctgcca
     2221 ttggcggacc atgccgagga agcggttgtt gaggatgagg atctttacct ccgcgccaaa
     2281 ttgcatgatc gtgcccagct cctgcagggt catctggaag ccgccgtcgc cgatgatagc
     2341 gactacggtg cggtcgggtg cgccatattt tgcgccgatg gccgcgggga ggccgaagcc
     2401 catggttccg aggccgccgg aggtgacgtt gctcttcgac tggttgaagt gagcgtagcg
     2461 gctggccacc atctggtgct ggccgacgtc ggtgacgatg acggcgtcgc cgcccgttag
     2521 ttcgttgagt actttcatta cctcacccat ggtcatttcg gccgtggtgg ggttcagttc
     2581 gtcatggata acggcttcta cctcctgttg gtagcagtct ttgaaacgct ggtgccattc
     2641 ggggaaggtg cggcgttcga ccagctccgt gagcaggggc agggtctctt tgcagtcgcc
     2701 ccagacgggt acggtggctt ttacgttctt atcgatctcg gcggggtcga tgtcgagatg
     2761 gacgaccctg gcctgtttgg cgtatttgtc gaggcggccg gtgacgcgat cgtcgaagcg
     2821 catgccgatg gcgatgagca cgtcgcagtc gtttgtgagg aggttggggc cgtagttgcc
     2881 gtgcatgccg agcatcccga cgttcaatgg atgatcggta gggagtgcgc tgaggccgag
     2941 gagggtccag gccgcgggga taccgctttt ttcgacgaag gcccggaatt cctgttccgc
     3001 cttaccgagg atgacgccct ggccgaagag gatgaagggg cgtttagcct cgtttatcag
     3061 tgcggcggct tttgcgatgt attccttacg gacgatgggt tttgggcgat agctgcggat
     3121 atgttcgcat ttggtatagc cttcgtagtt gaatttctgg atctgggcgt tcttggtgat
     3181 gtcgatgagc acgggtccgg ggcggccgct gccggcgatg tagaaggctt ttgccagtgc
     3241 ggcggggatc tcggtggcgt cggtgacctg gtagttccat tttgtgacag gcgtggtgat
     3301 attgatcacg tctgtttcct ggaaggcgtc tgtcccgagg aggtgggcga agacctggcc
     3361 ggtgatacat acgacggggg tgctgtcgat catcgcgtcg gcgaggccgg tgacgaggtt
     3421 ggtggcgccg ggaccgctgg tggcgaatac gacgccggtg cgcccggaag tgcgggcata
     3481 gccttgtgcg gcgtggatgc cgccctgttc gtggcggaca aggatatggt tgagtctgtc
     3541 gcggtagtca tagagagcgt catagatcgg catgatcgcg ccgccggg
//
LOCUS       DATGFG010000161         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_999946, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000161 DATGFG010000000
VERSION     DATGFG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999946"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2210)
                     /locus_tag="VKQ52_02995"
     CDS             complement(<1..2210)
                     /locus_tag="VKQ52_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86175.1"
                     /translation="MTGVLLLLLPVLVRGQKLDSMLNVYANNYPQEKVYVQFDKNLYH
                     EGETIWFKAYIFSGADPSLYSKNFYAELSDASGAILQRKVYPISESSTAGNFDLPRQV
                     KGRHLHFRGYTTWMTNFDTAFYFEKDLRIFDRQDSVLTVAIVPRQTRLQFFPEGGDLV
                     AGVEGMVAFKANDQFGLPVTVRGVIQDKSGKELLNFASEHDGMGRFPITPDKGDSLTA
                     VWQDEKGIEHRTGLPAVRSLGVVVRAIPGNQKVLFSVARSAEGGPDLKHLVIIAHMHQ
                     HLVYKAKINLEENFMSGGTIPTGQLPSGVLQLTVFTSEMMPVAERVIFVNNHDFLFNP
                     DMAMQLKSVARRGKNSIVVSVPDTLRSNLSLSVTDATADGLFPGDDNIVSRLLLTGDL
                     KGTVHNPYYYFAGTADSLTAQLDLVMMTHGWRRFKWESLARGQVPVIRNPEQDYLSMR
                     VEVLGVDPYKIAKDESLNVIMSKKDSSTQMYSIPHISGTKFGVTGLVFYDTVKAYYMF
                     NVNHALSNQAAVTFNTGLVNGARSVKPLSTAFEGWTAEDSAYLRRNRYIVQEEARVKP
                     IDDAKSKTLATVTVKGHIKSDAEKLDERYASGLFSGGDAQTFDLVNDNLASAYPDIFT
                     YLQGKVAGLQITPGNGPGGTPSLTWRGGRPGLYLNEMQTDAQQLQSVPVTDIALVKVF
                     RPGSGVGFGGGSGGTIAIYTKKGGDEKRNDPNIKGLDRAILIGYAMPKEFYVPN"
     gene            2346..>2594
                     /locus_tag="VKQ52_03000"
     CDS             2346..>2594
                     /locus_tag="VKQ52_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86176.1"
                     /translation="MQKIADVKFSAAVLQWDQETYLPQKGAEFRARQLATLSEIGHEW
                     FIDPGLGQLLEELHSRSDLRDDEQRNVALSLEDYKKQKK"
BASE COUNT          632 a          795 c          608 g          559 t
ORIGIN      
        1 taattgggca catagaattc cttcggcatg gcatagccga tcaggatagc ccggtcaagg
       61 cccttgatat tggggtcgtt cctcttctca tccccgccct tcttggtgta gatggcgatc
      121 gtcccgcccg agccgccgcc aaagcccaca cccgatccgg gccggaagac cttcaccagc
      181 gcgatatccg tcaccgggac actctgcagc tgctgggcat ccgtctgcat ctcgttaaga
      241 tagagaccgg gcctcccgcc ccgccatgta agcgatggcg tccctccggg tccgttgccg
      301 ggcgtgatct gcaaccccgc taccttgccc tgcagataag taaagatatc cgggtaagcc
      361 gatgcaagat tgtcattcac caggtcgaag gtctgggcat cccccccgga aaacaacccc
      421 gaggcataac gctcatccag tttctctgca tccgacttga tatgcccctt caccgtcacc
      481 gtggccaggg tcttggactt cgcatcgtcg atcggcttca ccctggcctc ttcctgcacg
      541 atataccggt tcctccgcag ataggccgag tcctccgccg tccaaccctc aaaagccgtg
      601 gacaacggct tcaccgatct cgcaccgttg acaagccccg tattaaaggt caccgccgcc
      661 tggttcgaca gggcatggtt gacattgaac atatagtacg ccttcaccgt gtcatagaac
      721 acaagacccg ttacgccgaa tttggtcccc gatatatggg gaatggaata catctgcgta
      781 ctcgaatcct tcttgctcat aatgacattc agggattcgt ccttggcgat cttataagga
      841 tcgacaccga gcacctccac ccgcatcgac aggtaatcct gctcggggtt cctgatcaca
      901 ggcacctgtc cccgcgcaag cgattcccat ttgaaccggc gccagccatg cgtcatcatg
      961 acaagatcca gctgcgctgt caggctgtcg gccgtaccgg caaaataata atagggattg
     1021 tgtaccgttc ccttcaggtc acccgtcagc aataatcgcg aaacaatatt gtcatcacca
     1081 gggaaaagac cgtcggccgt agcatccgtc accgataggg acaggttgct ccgcagggta
     1141 tcgggtacac tgacgacaat actgttcttt ccccgccggg ccacgctttt caattgcatc
     1201 gccatatcgg ggttaaagag gaagtcgtga ttgttgacaa agattacccg ctccgccaca
     1261 ggcatcatct ccgaagtaaa gaccgtcagt tgcagtaccc cgctgggcaa ctgccccgtc
     1321 gggatcgtac ccccgctcat aaaattttct tccaggttga tcttcgcctt atacaccaaa
     1381 tgctggtgca tatgcgcgat gatgacaaga tgcttcagat cgggaccgcc ctcggcgctc
     1441 ctggcaacgg aaaaaagaac cttctgattg ccggggatcg ccctgaccac aacccccagg
     1501 gacctcaccg ccggcagacc cgtacggtgt tcgatcccct tctcatcctg ccacacggcc
     1561 gttagcgaat cacccttatc gggagtaatg ggaaaccggc ccatcccgtc atgttccgat
     1621 gcaaaattca acaactcctt cccactcttg tcctgtatca cacccctgac ggtcaccggc
     1681 agcccgaatt gatcgttcgc cttgaacgca accatcccct ctacccccgc aacaagatca
     1741 ccgccttcgg gaaagaattg cagcctggtc tggcggggaa cgatcgccac cgtcaacaca
     1801 ctgtcctgcc tgtcaaatat gcggagatcc ttctcaaaat agaacgcggt atcaaaattc
     1861 gtcatccacg tcgtataccc cctgaaatgc aaatgccggc ccttcacctg cctgggcaga
     1921 tcgaaattgc ccgcggtact cgactccgag atgggatata ccttccgttg aaggatagcg
     1981 cccgaagcgt cggacaactc cgcataaaaa ttcttactgt aaagagaagg atcggcgccc
     2041 gaaaaaatat aggccttgaa ccagatcgtc tccccctcat ggtagaggtt tttatcaaac
     2101 tgaacataga ccttctcctg gggataattg ttcgcgtaga catttaacat ggaatccagc
     2161 ttctgcccgc gcaccaacac cggcaataac aatagcagaa caccagtcaa aaagcagcaa
     2221 ttttttttca tactattcaa aatgcggtta atttcggaat gttttccctc ttttgggaag
     2281 aaaaaaatgt taaaatggtt ttaacaaagt cttcgcaaga cctttacagc gaatatacag
     2341 cgcgaatgca aaagatcgcc gatgtaaaat tttcggcagc cgtgctccaa tgggaccagg
     2401 aaacctatct gccccaaaaa ggggcggaat tccgggcgcg ccagctcgcc acgctctccg
     2461 agatagggca cgaatggttc atcgaccccg ggctcggcca attactggaa gaactgcatt
     2521 cccgcagcga tctgcgggac gacgagcaac gcaacgttgc cctgagcctg gaagattata
     2581 aaaagcaaaa gaag
//
LOCUS       DATGFG010000162         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1455280, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000162 DATGFG010000000
VERSION     DATGFG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455280"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VKQ52_03005"
     CDS             complement(<1..427)
                     /locus_tag="VKQ52_03005"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLZ86177.1"
                     /translation="MVIVLFFLAHWFLSLFSQTFFLHRYASHKMFKMNKFWERFFYTF
                     TFVTQGSSFLNPRAYAILHRMHHAYSDTEKDPHSPYFIKDVWGLMIKTKNIYLDYLKY
                     KLEPEPAFRDRYPTWAPVDRLSDLWVTRIAFGIGYTAFYV"
     gene            complement(505..2988)
                     /locus_tag="VKQ52_03010"
     CDS             complement(505..2988)
                     /locus_tag="VKQ52_03010"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HLZ86178.1"
                     /translation="MQKSWFRPLIPHAIAIGVFFLVAAIYCKPIFEHKTLAPNDTAGW
                     KAMAQNSFQYKETHGQFPLWTESLFSGMPAYQIAMDSPSFSPQYLIYDLLTLGLPKPA
                     NFFFLACICFYFLALALRLNPYVGIITALAYGYSTYNPAILVVGHETKMQAIAIMPAF
                     LASLILLFEKKYWLGVATLALFTALYIAANHPQIVYYGIIAAAFMTVAYAIRWIKEKD
                     YRHMIMVAALGAVGIVIGIACNAVVTLTTMDYAKASLRNGSDLATPGGAVTKTGLSQD
                     YALSYSMFKSEAFTLLVPKIFGGSDVDAQITADDSKTITALQSMPPQLGQQLSNYIRS
                     YWGGIGTTAGPAYAGAVICLFALLGFFLLDGKHKWWILAAGVLTILMSWGGYFAGFNG
                     ALLKILPGYNKFRAPSVIIVVPTLLLCILAALTLNRLVALSAADRDATWKKYKKGLYL
                     TGGVFVILFVLYSSFDYAGNSDKGLQQATATMQPQVQEYVRTFVHAIREDRQSLFLNS
                     IFRSLLYIAVAAAIGFFWIKGKIKPLLALGIIGALAFIDLITVDSQYLSDDKYVDVEE
                     AQTPFQPTAADQQIMADKGYYRVFDLREGVENITNSSPTPYFHRSITGYHPAKLSIYQ
                     DLIENQLYKYPNCQPVLDMLNTKYLLLPTNTGRDSATLNPGALGPVWLVQGVRYADSA
                     RAVMDALTGLDTKDTAILYTADRSTGIQTNPNPGDTISLTRNDNDEMAYHSTTKGPRF
                     AVFSEVYYNRGWQAAIDGKPAPILRTDYVLRGLALPAGDHTINFSFHPASYYTGRTVQ
                     IVASLLLLALLILAGIQTWKTNPVRIKQQ"
     gene            3078..4004
                     /locus_tag="VKQ52_03015"
     CDS             3078..4004
                     /locus_tag="VKQ52_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86179.1"
                     /translation="MKKLFNRVFFRAMFGDFGFRNPYRFRQYEYPAYFQYRIGPYETG
                     HTLCFRRQPYLELYKNEIFAKMQEYSGYDVARYLEFHYAAFADKRRFLHFVWYEVTER
                     LKRDYSKKTSYYRCLEVAREWVEKEMRRVDVGVEEEGGGEGPGASEVSGSPGVGPGSA
                     ESGGSPVAGPGMAGVGAGSGLSDSGGSPVIGAGLAELIEPSLSEVTRSFAGKISVNNM
                     HHLDRMIQLFVLLQQVEAPESGVAPLFRSFTDIDLAAILRQFDGFRDKKLNTLQKKIS
                     DTSADIRLKKNQRVAKMREMMAEFFYGTDIRP"
     gene            4063..4404
                     /locus_tag="VKQ52_03020"
     CDS             4063..4404
                     /locus_tag="VKQ52_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86180.1"
                     /translation="MYSNKSLIKDRTLQFYCTKVQDRYVRAMAKKAGLAVPEYLRRQA
                     LAGFVPKEKTLPPEVLSFCGQLRHLAGLLHPIARKRLDGDDLNALERAELKQTKAQID
                     DILSHIKKSLS"
     gene            4401..>4734
                     /locus_tag="VKQ52_03025"
     CDS             4401..>4734
                     /locus_tag="VKQ52_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86181.1"
                     /translation="MIGHIDFNVSFQYSIGYCLEDKKQTQRQTQGQTQSQTQRQTEGQ
                     TPEVAYRNRAEVLYYNQCFGDKRELIQQFEEVQKLNRNIVKPVLHLSLSAAPKDEISK
                     SRWVDLARD"
BASE COUNT         1010 a          983 c         1659 g         1082 t
ORIGIN      
        1 ccacatagaa ggcggtgtag ccgatgccga aggcgatgcg ggtgacccag aggtcgctga
       61 ggcgatcgac cggggcccag gtggggtagc ggtcgcggaa ggcgggttcg ggttccaatt
      121 tatatttaag gtaatcgagg tagatgttct tggtcttgat catgaggccc cagacatcct
      181 ttatgaaata gggggagtgg gggtctttct cggtgtcgct ataggcgtgg tgcatccggt
      241 ggaggatggc gtaggcgcgg ggattcagga aggacgagcc ctgggtgacg aaggtgaacg
      301 tataaaagaa tctttcccag aatttattca tcttgaacat cttgtgcgaa gcatagcggt
      361 gcaggaagaa cgtttgtgaa aatagggaca ggaaccagtg tgctaagaaa aacaatacaa
      421 taaccataga gaaaaaaagt aatagatgcc ttaaaagagg tacgaaatta ggtgcttttt
      481 agtttactgc gtggttttgt ttggctattg ttgtttgatc ctcacggggt ttgttttcca
      541 ggtctgaata ccggccagga tcaggagggc caggaggagg aggctggcga cgatctggac
      601 ggtcctcccg gtatagtagg aggcggggtg aaaggagaag ttgatggtat ggtcgccggc
      661 gggcagggcg aggccccgga gcacgtaatc ggtgcggagg atgggagcgg gtttgccgtc
      721 gatcgcggcc tgccatccgc ggttgtagta gacttcgctg aagacggcga agcgggggcc
      781 ttttgtggtg gagtgatagg ccatttcgtc gttatcgttg cgggtgaggg agatagtgtc
      841 acccgggttg gggttagttt ggatgccggt gctgcggtcg gcggtgtaga ggatggcggt
      901 atcttttgtg tccaggccgg tgagcgcgtc catgacggct cttgcgctat cggcgtagcg
      961 gacgccctgg acgagccaga cggggccgag ggcgccgggg ttgagggtgg cggagtcccg
     1021 accggtgttg gtgggcagga ggaggtattt tgtgttaagc atgtccagca cgggctggca
     1081 attgggatat ttatagagtt ggttctcgat cagatcctgg tagatgctga gtttggccgg
     1141 atggtagccg gtgatggaac ggtggaagta gggggtaggg ctgctgttgg tgatgttctc
     1201 gactccttcg cggaggtcga agacgcggta gtagccttta tccgccatga tctgctggtc
     1261 ggcggcggtg ggttgaaagg gggtttgtgc ttcctccacg tctacgtatt tgtcgtcgct
     1321 gaggtactgg gagtcgaccg tgatcagatc gataaaggcc agggcgccga tgatcccgag
     1381 cgcaagaagg ggttttattt tgcctttgat ccagaagaat ccgatggcgg cggcgacggc
     1441 gatatagagg aggctgcgga agatactgtt caggaagagg ctttgccggt cttcccggat
     1501 ggcgtggacg aatgtccgga cgtattcctg cacctggggc tgcatggttg ccgtcgcctg
     1561 ctggaggccc ttgtcggagt tgccggcgta gtcgaagctg gaatagagta cgaagaggat
     1621 gacgaagacg ccgccggtga ggtagaggcc ttttttatat tttttccagg ttgcgtcgcg
     1681 atcggcggcg gagagggcga ccaggcggtt gagtgtgagg gccgccagga tacagagcag
     1741 gagggtgggg acgacgatga tgacgcttgg cgcccggaat ttgttatagc cggggaggat
     1801 cttcaggagg gcgccgttaa aaccggcgaa atagccgccc cagctcatga ggatggtgag
     1861 gacgcctgcc gcgaggatcc accatttgtg tttgccgtcg aggaggaaga agccgagcag
     1921 ggcgaacagg cagatgacgg cgccggcgta ggcggggcct gccgtggtgc cgataccgcc
     1981 ccagtagctt cttatgtaat tggacaattg ctgacccaac tggggtggca tgctttggag
     2041 ggcggtgatc gttttggaat cgtcggcggt gatctgggcg tcgacgtcgc tgccgccgaa
     2101 tatctttggt acgaggaggg tgaaggcctc cgatttgaac atgctgtagg agagcgcata
     2161 gtcctggctg aggccggtct ttgttactgc tcccccggga gtggccaggt cacttccgtt
     2221 gcggagggag gcctttgcgt agtccatggt ggtaagggtt accacggcgt tacaggcgat
     2281 gccgatgacg atgccgacgg ccccgagggc ggcgaccatt atcatgtggc gatagtcttt
     2341 ttcttttatc cagcgaatgg cataggcaac ggtcataaag gcggccgcga tgataccata
     2401 ataaacgatc tgcgggtggt tggcggcgat ataaagggcg gtgaacaagg cgagggtggc
     2461 tacgccgagc cagtattttt tctcgaagag caagatcagg gaggcgagga aggcgggcat
     2521 gatggcgatg gcctgcattt ttgtctcgtg gccgactacg aggatcgccg ggttataggt
     2581 ggaatacccg taggccaggg cggtgatgat ccctacgtag gggtttagcc gaagggcgag
     2641 ggccaggaaa tagaagcaga tgcaggccag gaagaagaag ttggccggtt ttggcaggcc
     2701 gagtgtcagg agatcataaa taaggtattg cggggagaag gagggcgagt ccatggcgat
     2761 ctggtaggcg ggcatgccgc tgaagaggct ttcggtccat agggggaact ggccatgggt
     2821 ttccttgtac tggaaggaat tttgtgccat ggctttccag ccggcggtat cattgggggc
     2881 cagggtcttg tgttcgaaga tgggtttgca gtagatagcg gcaaccagga agaagacccc
     2941 gatggcgatg gcatggggga taagcgggcg aaaccaggac ttttgcatgg gagcaatatt
     3001 aggcaattcc ggttattttt ttaccggggc tttggggtaa attggggatc aaacctaatc
     3061 ttatcgttat gttgcccatg aaaaagttat ttaaccgggt attcttccgg gccatgtttg
     3121 gcgattttgg attccggaac ccttaccgtt tccgtcagta cgaatatccc gcctattttc
     3181 agtaccggat cggtccgtac gagactggtc atacgctgtg tttccggcgg cagccttatc
     3241 tcgagctata caagaatgag atctttgcca agatgcagga atattcgggg tatgatgtgg
     3301 ccaggtatct cgaatttcat tatgccgctt ttgcagataa gcggcgcttt ctccattttg
     3361 tctggtatga ggtgacagaa cgactgaaac gggactattc aaagaagact agctattacc
     3421 ggtgtctgga ggtcgcccgg gagtgggtgg agaaggagat gcgaagagtg gatgtgggtg
     3481 tagaagagga gggaggcggt gaggggccgg gggcttcgga ggtgagtgga tcgccgggag
     3541 ttgggccggg gtcggcagag tcgggtggat cgccagtggc cgggccgggg atggcgggag
     3601 ttggtgcggg ttcgggcttg tcggactcgg gtggatcgcc ggtaattggt gcggggctgg
     3661 cggagttgat cgaacccagt cttagcgagg tgacgcggtc gtttgcgggg aagatcagtg
     3721 tcaataatat gcaccacctg gaccgaatga tccagctttt tgtgttattg cagcaggtgg
     3781 aggcgccgga atcgggtgtg gccccgctgt tcaggtcctt tactgatatc gatctggcgg
     3841 ccattctccg gcagtttgat ggattccggg acaagaagct gaatacctta cagaaaaaga
     3901 tcagcgatac cagcgccgat atccggctta agaagaacca gcgggtggcg aagatgcgcg
     3961 agatgatggc cgagttcttt tatggaacgg atatccgtcc atgatgcatc catgactcac
     4021 ttcggtatat agcttgtggt ttgttactac aaaattgaaa gtatgtatag taacaaatcg
     4081 ttgatcaagg atcgcacgct acagttctat tgtacgaagg tacaggatcg atatgtccgg
     4141 gccatggcca agaaggcggg cctggcggtg ccggaatatt tgcggcgcca ggcgcttgcg
     4201 ggttttgttc ccaaggagaa aaccttaccg cccgaggtat tgtcgttctg cggacagtta
     4261 cggcatttgg cgggtttgct gcatcctatc gcgcgaaagc ggctggatgg ggatgatctc
     4321 aatgcgctgg agcgggcgga gctaaagcaa acgaaggcgc agatcgatga cattttatca
     4381 cacattaaaa aatccctatc atgattgggc atatcgattt taacgtttcc ttccagtatt
     4441 cgatcgggta ctgcctggag gacaagaagc agactcagcg tcagacgcag ggtcagaccc
     4501 agagccagac gcagcgtcag acggaggggc agacgccgga agtggcgtac aggaaccggg
     4561 cggaggtgct ttattataac cagtgttttg gggacaagcg ggagctgatc cagcagttcg
     4621 aggaggtgca gaagctgaac cggaatatcg tcaagcctgt gttgcatctt tcgctgagtg
     4681 cggcgccaaa ggatgagata agcaagagcc gatgggtcga tcttgcacgg gatt
//
LOCUS       DATGFG010000163         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_545040, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000163 DATGFG010000000
VERSION     DATGFG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545040"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VKQ52_03030"
     CDS             complement(<1..1088)
                     /locus_tag="VKQ52_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HLZ86182.1"
                     /translation="MPTAHDDFDWSIDKRNVAVYTREEKEKYDKVYEGTFVAITDGEL
                     VKGLVVGLTKTDVVVNIGFKSDGLVSLNEFRDLQNLRTGDEVEVMVVEKEDREGHLHL
                     SRKQARITRAWEKIVEVHKTGEVVTGTVTSKTKGGLIVDVFGMETFLPGSQIDVKPVT
                     DYDQFVGKTMEFKVVKINEAIKNAVVSHKALIESDIEAQRAEIISKLEKGQVLEGTIK
                     NITDFGAFMDLGGLDGLLYITDISWGRISHPSEVLKLDQKLQVVVLDFDDGKKRISLG
                     LKQLTPHPWDVLPETIKEGEIVKGKVVNIEDYGAFLEIMPGVEGLVHVSEITWANTPI
                     NAKEFFKLGDEHQAKVVTLDKDARKMSLS"
     gene            complement(1251..2567)
                     /locus_tag="VKQ52_03035"
     CDS             complement(1251..2567)
                     /locus_tag="VKQ52_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLZ86183.1"
                     /translation="MQQKNLIVIGGGAAGIFCAVNAARMSPGLRVTVLEKTGKLLSKV
                     KVSGGGRCNVTHACFDIGEMARRYPRGQHFVRKAFHRWFTTDTIEWFRERGVELKTEE
                     DGRMFPVTDSSQTIIDCLMREVNSYGVEIWMHAEVGGVMADGGGSFRVRLADGRVLVA
                     DYVCVACGGYPKPGMFDWIRELGHSIEAPVPSLFTFNMPGDPIVGLMGLSMPAVQVKV
                     MGTKLVEKGPVLITHWGLSGPAVLRLSAWGARELADRSYSFDIQVNWIDMTEVHARER
                     MLDMRQERAGQAVAGRNPFGAPQRLWEYWLGVAGIGTDVRWADLPAREQNKLISLLCA
                     GTFAVKGKTTFKEEFVTAGGVRLAEVDPGSFQSRKVPGLYFAGEILDVDGVTGGFNFQ
                     HAWTSGWVAAASVAAACGAGAGEGGRGDGGHVGRGGFGDGSAGVDK"
     gene            complement(2577..3395)
                     /gene="map"
                     /locus_tag="VKQ52_03040"
     CDS             complement(2577..3395)
                     /gene="map"
                     /locus_tag="VKQ52_03040"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HLZ86184.1"
                     /translation="MIHYKTPAEIELMRDSALLVSKTLTEVAKVLKPGTTSLELDKLI
                     GDFIRDHGAIPSFLNYRGYPFSSCISVNDVVVHGFPNGQPLREGDIISVDIGVIKKGY
                     HGDHAYTFAIGDPGPEVVQLIRVTKESLYKGIEKAVAGGRVGDISYAIEEHTAKKYGY
                     GVVRELVGHGLGKHLHEDPQIPNYGRRGTGAKLKEGLVLAIEPMINLGKREVYTESDG
                     WTVRTSDHQPSVHFEHDVAVRKGKADILSDYSIIEAAEVANPHLFTGEAATLSS"
BASE COUNT          746 a         1207 c          731 g          741 t
ORIGIN      
        1 gacaagctca tcttgcgggc atccttgtca agcgtaacca ccttcgcctg gtgctcatcg
       61 cccagtttga agaattcctt cgcattgatc ggggtattcg cccaggtgat ctcgctcacg
      121 tgtaccagac cttctacgcc cggcatgatc tcgaggaatg caccataatc ttcgatattg
      181 accaccttac ccttgacgat ctcgccttcc ttgatcgtct caggcagcac atcccaggga
      241 tgcggggtca gctgtttcag acccaggctg atacgcttct tgccatcgtc gaagtccaac
      301 accaccacct gcagcttctg gtccagcttc agcacctcgc tcggatggct gatacgtccc
      361 cagctgatat cggtgatata cagcaggccg tccagaccac caaggtccat aaacgcgcca
      421 aaatcggtga tattcttgat cgtaccctcc agcacctggc ctttctccag cttgctgatg
      481 atctccgcac gttgtgcttc gatatcgctt tcgataagag ccttgtggga tacaacggcg
      541 ttcttgatgg cttcgttgat ctttacgacc ttgaactcca tcgtcttgcc gacaaattga
      601 tcatagtcgg taacaggctt cacgtcgatc tgcgatccgg gcaggaaggt ctccatgccg
      661 aatacatcga cgatcaggcc acccttggtc ttgctggtaa cggtacccgt aaccacttcg
      721 ccggtcttgt gaacttccac gatcttctcc caggcacggg tgatccgggc ttgcttccgg
      781 ctgaggtgca ggtgaccctc gcggtcttcc ttctctacaa ccatcacttc cacttcatca
      841 ccagtccgca ggttctgaag gtcccggaac tcgttaagcg atacaaggcc atcgctctta
      901 aagccaatgt tcactaccac atcggtcttg gtcagaccca ctaccaggcc cttgaccagt
      961 tcgccgtccg tgatagctac gaatgtccct tcatacacct tgtcatactt ttccttttct
     1021 tccctggtgt aaacggctac attccgtttg tcaatgctcc agtcaaaatc atcatgtgcg
     1081 gtcggcacag gcacaggtgc ttttgttgtc gctgtagcct cggcagtagc agtcgccact
     1141 tcctgagtct cctgtgattc agcgtttgag ttaacaatat tgttttcaga cataataaaa
     1201 ccccggtttt tttgatcggg gttgcaaaga tacgaaaaaa tggcgatttt tcacttatcg
     1261 acacccgccg aaccatcccc aaaaccaccc cttccaacat gccccccatc accccttccg
     1321 ccctcgcccg caccagcccc acacgccgcc gcaacactcg ccgccgctac ccacccactc
     1381 gtccacgcat gctgaaaatt gaacccgccg gtaaccccat ccacatccag gatctccccg
     1441 gcaaaataca accccggcac cttccggctc tgaaagctcc ccgggtccac ctccgccagc
     1501 cgaaccccgc cggccgttac aaactcctcc ttaaaggtcg ttttcccctt cacggcaaag
     1561 gttccggcac aaagcaggct gatcagctta ttctgctccc gcgccggcag gtcggcccag
     1621 cgaacatcag tcccgatccc ggcaacccca agccaatact cccacagccg ctgcggcgcc
     1681 ccgaacggat tccggcccgc caccgcctgc cccgcccgct cctgccgcat gtccagcatc
     1741 cgctcccggg catgcacctc cgtcatatcg atccaattaa cttgaatatc aaaggaatag
     1801 ctccgatctg caagctccct cgccccccag gccgaaagcc gcagcaccgc cggcccgcta
     1861 agcccccagt gcgtgatcag caccggcccc ttctccacca gcttcgtccc catcaccttc
     1921 acctggacag ccggcatcga cagccccatc aacccgacga tcgggtcacc gggcatatta
     1981 aaggtaaaaa gcgagggcac cggcgcctcg atggaatgcc ccaattcccg gatccagtca
     2041 aacatccccg gcttgggata ccccccacat gcaacacaca cataatcagc aactagaacc
     2101 cgcccatccg ccagccggac ccgaaaacta cccccaccgt ccgccatcac tccccctacc
     2161 tccgcatgca tccatatctc caccccatag gaattcacct cccgcatcag gcaatcgatg
     2221 atcgtctggg acgaatcggt caccgggaac atcctgccgt cctcttcggt cttcaactca
     2281 actccccgct cccggaacca ttcgatggta tccgtcgtaa accatcgatg aaaagccttc
     2341 cgcacgaaat gctgtccacg gggatacctc cgggccatct ccccgatatc gaaacaggca
     2401 tgcgtcacat tgcaacgccc acccccgctg accttcacct tgctcagcag cttccccgtt
     2461 ttctccagca cggtgacgcg caaacccggg ctcatccgcg ccgcattcac agcacaaaag
     2521 atacccgcgg ccccgccgcc aataacaata agattttttt gctgcatacc ggcccgttaa
     2581 gacgacagcg tagctgcctc tccggtaaaa agatgaggat tagccacctc ggccgcctcg
     2641 atgatactat aatccgaaag gatatccgcc ttccccttcc gtaccgccac atcatgctca
     2701 aaatgcaccg aaggctgatg atccgacgtc cgtaccgtcc agccatcact ctccgtgtaa
     2761 acctcccgct tgcccaggtt gatcatcggc tcgatcgcca gtaccagccc ttccttcagc
     2821 ttggccccgg tgccccgccg cccatagttc gggatctgcg ggtcttcatg caggtgcttg
     2881 ccaagcccgt gtcccaccaa ttcccggacc actccatatc cgtacttctt cgccgtatgc
     2941 tcctctatcg cataggagat atcccccacc cgcccgccgg cgacagcctt ctcgatcccc
     3001 ttgtacaggg actccttcgt cacccggata agttgcacca cctccggacc cggatcgccg
     3061 atcgcgaacg tgtacgcatg atccccatga taccccttct tgataacccc gatatccacc
     3121 gaaatgatat ccccttcccg caacggttgc ccattgggaa aaccatggac gacgacatcg
     3181 ttcaccgaga tacaggaaga aaagggataa ccccggtagt tgagaaaaga aggaatggcg
     3241 ccatgatcac ggataaaatc cccgatgagt ttgtctaatt ccagggacgt cgtccccggc
     3301 ttcagcacct tggccacttc ggtaagggtc ttgctgacca gcaacgcgct gtcgcgcatc
     3361 aattcgattt cagccggtgt tttgtaatgg atcatactac tatcgaaaaa gacctgttag
     3421 gaggt
//
LOCUS       DATGFG010000164        12010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1454992, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000164 DATGFG010000000
VERSION     DATGFG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12010
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454992"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VKQ52_03045"
     CDS             complement(<1..272)
                     /locus_tag="VKQ52_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HLZ86185.1"
                     /translation="MSRFLTLTVVSALVAAVLLGYFAPATAVQMKFLGDWFVNVIKVF
                     IPFIIFLTITSGITGMSDLKKVGRIGVKALLYFEVVTTLSLAIGIF"
     gene            complement(326..1675)
                     /gene="kynU"
                     /locus_tag="VKQ52_03050"
     CDS             complement(326..1675)
                     /gene="kynU"
                     /locus_tag="VKQ52_03050"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HLZ86186.1"
                     /translation="MQQEIASALDYAQKRDREDELRGFRSRFYFPKREEGELLYFCGN
                     SLGLQPVSAEESIRQELTDWREMAIGGYWQARNPWMTYPASLRAPLARIVGSGEQEVA
                     VMNALTVNLHLLMLSFYRPTPRRFKILMEAGAFPSDQYAVETQVKWHGFDPREAIIEV
                     APREGERLIREEDVVAAIGREKGSLALVLFGGLNYYTGELFDMQAITAAAHEAGAHAG
                     FDLAHAVGNVPLRLHDWEVDFAVWCSYKYLNGGPGAAGGAFIHERHAQDTSLLRLGGW
                     WGNEGSGRFRTDRQSLSSGQAASGGSGGFVPKPTAEGWSMSTAQVFNMVCLKASLAIF
                     EEAGIGRIQAKSRELTAYLASLLDPLAGGTFRVITPAEPERRGAQLSLYFEDAARGKA
                     IQQQLMEEGIVTDYREPGVVRVAPAPLYNSFEDVYRFCHIFRQLLDGVQSACEAGRH"
     gene            complement(1729..2781)
                     /locus_tag="VKQ52_03055"
     CDS             complement(1729..2781)
                     /locus_tag="VKQ52_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86187.1"
                     /translation="MKKHIRLLNLAAVLLVLSAPKMTLAANGDEDVRKQKMIHRNYAV
                     TADDKLEIDNQFGNVVVSTWDQQEITVDIEISARASTEERAQEIMNRIDVKDAKDGHS
                     IWFKTKVGEIHNNNNGNRGRNDNQRGFYIDYVIHMPATNRLAIENSFGKTEIPAFKAL
                     VTLTSKFGSLVTGKLDNVDQIDVEFGRAYIASVTNGKVAFKFNKESRIGKVDGNVKIT
                     SEFSQNVQFIVANDIQELAIFESYSGVRVVVDKALSAQFDVHTSFGKFHNESEFNINE
                     QKEDDSYGPHFDKDYTGQSGDGKARIKIKSSFGTVRLCYTGDKDRDRDDEGDDDKDKE
                     KHKNKVKDKDTDDEAQ"
     gene            complement(2756..3475)
                     /locus_tag="VKQ52_03060"
     CDS             complement(2756..3475)
                     /locus_tag="VKQ52_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86188.1"
                     /translation="MNSRLEQFVQDHREEFDAEIPDPKVLEKILKDVDPGKKKQAPLV
                     RPDWRRWSAAAAVVLLIAGGVWYFKSRTGEGPQTIVRTNTAPATTTPAGEGSKATNPV
                     AQAPVKDSTQAAKEPTLASIRPQDDKNEKETAELDNSMNEEMYHYARLVEIKHNQLKA
                     IEKDEPLLYRQFASDVSKLDSVYHSLQNQLPQNPNREQLLEAMLQNLQLQMGLLNHQL
                     DIIKQINHSKKTEYEKAYKTT"
     gene            complement(3472..4026)
                     /locus_tag="VKQ52_03065"
     CDS             complement(3472..4026)
                     /locus_tag="VKQ52_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLZ86189.1"
                     /translation="MELTEPILHDELVERCKRGDSRAFGELYQKYSRAMYNTSLRIVN
                     HTGDAEDVLQEAFTDAFRSLEDFHYKSTFGAWLKRIVINKSINQLRKRKMDLIDIEKT
                     NIGHLPDDEGPEENEIALKVEDIKKAVGLLPNGYRTVLTLYLFEGYDQEEIAEILQVS
                     HATVRTQYMRAKQKLLQYLKQGVS"
     gene            4157..5353
                     /locus_tag="VKQ52_03070"
     CDS             4157..5353
                     /locus_tag="VKQ52_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLZ86190.1"
                     /translation="MPITSSSKIDVFQSPDYYELDELLTEVHKMVRDSVRNYVKKEIS
                     PIIEDCAQKAEFPQQIVPQLGELGCFGPTIPEQYGGGGLDYISYGLMMQELERGDSGV
                     RSTASVQGSLVMFPIYEYGSEEQRRKYLPKLASGEWLGCFGLTEPNHGSDPGGMLTNI
                     KDAGDHYVLNGSKMWISNAPFSQVAVVWAKDEEGVIRGVIVERGMEGFSTPTTHGKWS
                     LRASATGELVFDNVKVPKENLLPNVKGLKGPLGCLTKARYGIAWGVVGAAMDCYDTAL
                     RYSLERVQFGRPIGGFQLQQKKLAEMLTEITKAQLLNWRLGTLMTAGKATPAQVSMAK
                     RNGCAIAADIARDARQILGGMGITGEYPIMRHMMNIESVITYEGTHDVHLLITGMDIT
                     GLNAFK"
     gene            5350..5886
                     /locus_tag="VKQ52_03075"
     CDS             5350..5886
                     /locus_tag="VKQ52_03075"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HLZ86191.1"
                     /translation="MKIFLIGFMGSGKTHWGQRLAEKLQLPFYDLDSLIIEKENKSVA
                     GIFEENGEEYFRFLEKETLESTTDQQESFILSCGGGTPCFFNNIEFMKKNGKVVWLNT
                     AVDVLRQRLQRERMSRPLIADVTEDDLQRYIIRKLSERRMYYQQADVTVSEEHTNLDE
                     LIRILLQNEQPDRLKEEL"
     gene            5883..6272
                     /locus_tag="VKQ52_03080"
     CDS             5883..6272
                     /locus_tag="VKQ52_03080"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HLZ86192.1"
                     /translation="MNRNFLSVAAILGALSVALGAFAAHKLKTMVGPDAVAIFETGVR
                     YQFYHVFALLFVAFLSEKIHNKWMIWAGNCFIMGIIFFCGSLYVLTALTIAENTHRTL
                     AGIATPIGGVFFIAGWVFLYLGVKSRP"
     gene            complement(6346..8262)
                     /locus_tag="VKQ52_03085"
     CDS             complement(6346..8262)
                     /locus_tag="VKQ52_03085"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HLZ86193.1"
                     /translation="MNILKACLSGLFLLLLRLDSQAQQSQPEVAADISQTGLLDDIAV
                     NTGYYFSNDRNISIRQLPSLPYRQNTRKYMHFMAPELMNKKPVLRFTLVNRSDSVQSV
                     FFCPGWLVDTIQLYRLVPGGPEGMHAEALPRVVPDNPDSLGYRKITLAAHDSATYFAT
                     LSYVRAATNSINPSLTRAKLIEEGITLQHKNKTSIDVITNLFAGIMLMMIFYSVSEYI
                     QSKKPEFLYYIGYSICISVLLFLKSTLHQVTTSFNFFFESFLDNIIFSVGYIFYIIFH
                     RKFLETSKHYKPLDRYLFWGSVGIAGLTVLYVLSYFFSADFRIANNLETAIKLVLLAT
                     SIIFIIKGLSYHNRLMNYIVMGNISLCLLSIISLVMISTNIKIASGVSVLNASLFHYE
                     VGITIETLFFLFALTYKNKMEIIVSIQKEEKMKLETERKEMEKQVAVLAAKQDERNRI
                     SVDMHDELGSGVTAIRLMSEIVKSKMKDQTLPEIDKISNSANELINKMNTIIWTMTSS
                     NDSVENLIAYIRSYAVEFFENTAIDCYFTMPASIPPREISGEKRRNIFLSVKEALNNV
                     LKHSQSSVVKINISVNERLIIEIQDDGIGINMEKLRKFGNGLNNMRKRMASIEGGFQI
                     ENQEGTRTTFYLQL"
     gene            8338..10914
                     /locus_tag="VKQ52_03090"
     CDS             8338..10914
                     /locus_tag="VKQ52_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HLZ86194.1"
                     /translation="MANRQKSKKSQKSSNKLKAEKEAQVTVGQLVKDERTHKITGAVF
                     LLLAIFLFIAFTSYLFTWKEDQDKVFHHGASVLLPSSNVKVSNLLGNLGALVSHEFFF
                     KGFGLASYFFCSIFFIIGVNLLFARKIFSISRNLRYVLAGLLYFSVAAAFITNGMDFP
                     WGGAVGDMISEWLTRFLGWIGTAALLLVAGLAYIIWRFNPVFKVPSLPKKENAAVPVP
                     EPLTEEKPEPKEKEKEKGKKGAKLFVDESFNNKGNVLKDDTGVVAIAPPPIEEEQEIE
                     FTVTEREPEEEPFSLIGAPLEEEEEEDDLDEEPAEEPAPPLKIPPIPPIAKTPLPGDL
                     QLEIKTVPPLEEPVMEGDSLLPSDLPDYEPTLDLRDYKYPGLDLLEAHGSEKIVQDPS
                     ELEANKNQIINTLKNYDISISKISATVGPTVTLYEIVPAAGVRISRIKNLEDDIALSL
                     AALGIRIIAPIPGKGTIGIEVPNVKKTVVSMRTLLSSDKFQHSNFNLPIAIGKKIDNE
                     NFIVDLASMPHLLMAGATGQGKSVGLNAILVSLLYKKHPSQLKLVLVDPKKVELSIYR
                     TIEKHFLAKLPGEEEAIITDTKKVIHTLNALCIEMDNRYDLLKEANARNIKEYNEKFI
                     KRRLNPQKGHQYLPFIVLVVDEFADLIMTAGKEVEMPIARLAQLARAVGIHLIIATQR
                     PSVNIITGTIKANFPARIGFKVSSKIDSRTILDAGGADQLIGKGDMLISYNGEITRLQ
                     CAFVDTPEVEEITQFIGKQRGYAEAYKLPEYVDEKEMEAKDFDLGERDPLFDEAARLI
                     VANQLGSTSLIQRKMKLGYNRAGRLMDQLEAAGVVGPGQGSKPRDVLIKTEMELDQYL
                     NP"
     gene            11018..11692
                     /locus_tag="VKQ52_03095"
     CDS             11018..11692
                     /locus_tag="VKQ52_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HLZ86195.1"
                     /translation="MNRKTFLFCLVLGAFGTLAHAQNNSLGKNDPDAKKVLDGLSTKL
                     KSFKAVQCGFTLTVNDAKDKVLGSKNGTIQIKGNKYHVSVVGGQDIFCDGKDIWTYDK
                     SSNEVTITKSDPTTQTISIDKIFTNFYDKDFLYKLNGESKAGARTLQEVELTPTDKTK
                     PFFKALLYIDKTQHTLASIKWFDKSGSKYTLTTKNLNGNAEVTDAQLAYNKAKFPGAE
                     EVDLRN"
     gene            complement(11694..>12010)
                     /locus_tag="VKQ52_03100"
     CDS             complement(11694..>12010)
                     /locus_tag="VKQ52_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HLZ86196.1"
                     /translation="MIIVPCSMGTLGRIATGVSSDLITRAADVVLKERRKLICVVRDT
                     PYNLIHIRNMETVTLAGGIICPATPSFYSLPKTIDEVAATVVDRVLDLAGLDISTFRW
                     GT"
BASE COUNT         2768 a         3551 c         3052 g         2639 t
ORIGIN      
        1 ataaagatgc cgatggcaag cgacagcgtg gtcaccacct cgaaatacaa caacgccttc
       61 accccgatgc gccccacctt cttcaggtca ctcatccccg tgatccccga tgtgatggtc
      121 agaaagatga taaagggaat aaagactttg atcacattca cgaaccagtc gccgagaaat
      181 ttcatctgca cggccgtcgc cggtgcaaaa taacccagca gtaccgccgc caccagcgcc
      241 gataccaccg taagagtaag gaatctgctc ataattgaaa ttgcggcaaa aaattgtcaa
      301 aaaataattt tttgccttaa tacggctaat gtctgcctgc ttcgcaggcg gattggactc
      361 cgtccaatag ttgtctgaag atatggcaaa accggtacac gtcttcgaat gaattgtaca
      421 acggcgccgg cgccacgcgc accacacccg gttcgcggta atcggtcacg atcccctctt
      481 ccatcagctg ctgctggatg gccttgccgc gggcggcatc ctcgaaatac agggacaact
      541 gcgctccccg ccgctcgggc tccgccgggg tgatcacccg gaacgttccg ccggccagcg
      601 ggtccagcag cgaggccagg taggccgtca gctcccggct cttggcctgg atccgcccga
      661 tgcccgcctc ctcgaagatc gccaggctgg ccttcagaca aaccatatta aagacctggg
      721 ccgtgctcat gctccagccc tcggcggtgg gcttggggac gaagccccct gagccgccgg
      781 aggcggcttg tccggaggac agactttgac ggtccgttct gaatcggccc gacccctcat
      841 tcccccacca gccgccaagc cgcaaaaggg aggtgtcctg cgcatgccgt tcatggataa
      901 acgcgccgcc tgccgcaccg ggcccgccgt tcaggtattt gtaagaacac catacggcaa
      961 agtctacctc ccagtcatgg agccgtaatg ggacattgcc tactgcatgc gccaggtcga
     1021 atcctgcgtg cgcgccggct tcgtgtgcgg cagcggtgat ggcctgcatg tcgaataatt
     1081 ctccggtata gtaattcagc ccgccaaaca acaccagcgc cagcgacccc ttctcgcgcc
     1141 cgatggctgc cactacatcc tcttcccgga taagccgctc cccctcgcgg ggcgccacct
     1201 cgatgatggc ctcccgcgga tcgaacccat gccacttcac ctgggtctcc acggcatatt
     1261 gatcggaggg aaaggcgccc gcctccatga ggatcttgaa gcggcgcggg gtagggcggt
     1321 aaaagctcag catcaacagg tgcaggttca ccgtcaacgc gttcatgacc gccacctcct
     1381 gctccccgct gcccacgatc ctcgccagag gagcccggag actcgccgga taggtcatcc
     1441 agggattgcg ggcctgccag tacccgccga tcgccatctc ccgccagtcg gtcaactcct
     1501 gccggatcga ctcctccgcg gacacgggct gcaggccgag ggagttgccg cagaaataca
     1561 gcaattcgcc ctcttccctc ttgggaaaat aaaaccggga gcgaaagcct cgcagctcat
     1621 cctcccggtc ccttttttgc gcatagtcca gcgccgatgc tatttcctgc tgcatgccat
     1681 cttgtttatt gcaagataag cattgctccc ataaatcaga tcgtgtagct attgcgcctc
     1741 atcatcggta tccttgtctt ttaccttgtt cttgtgtttc tccttatcct tgtcgtcatc
     1801 gccttcgtca tcccggtccc ggtccttgtc gccggtgtag caaaggcgca ccgtcccaaa
     1861 actgctcttg atcttgatcc tggccttgcc atccccggac tgcccggtat aatccttgtc
     1921 gaaatgcggg ccataactgt catcctcttt ctgctcgttg atgttgaact cactctcgtt
     1981 atggaattta ccaaagctgg tatggacatc gaactgcgcc gagagggcct tgtccaccac
     2041 tacccgtacc ccggaatagg attcgaagat ggccagttcc tggatatcat tggccacaat
     2101 gaactggaca ttctgggaga actcgctggt gatcttcaca ttcccatcca ccttaccgat
     2161 ccgggattct ttgttgaact tgaaggcgac ctttccgttg gtcaccgacg cgatatacgc
     2221 cctgccaaac tccacgtcga tctggtccac gttatccagc ttcccggtaa cgagactgcc
     2281 gaatttgctc gtcagcgtga ccagcgcctt gaaggcgggg atctcggtct tcccgaaact
     2341 gttctctatc gcgagccggt tggtcgccgg catgtgaatg acatagtcga tatagaagcc
     2401 gcgttggttg tcgttacggc cccggttccc gttgttgttg ttatggatct cgcccacctt
     2461 ggtcttgaac cagatggagt gtccgtcctt ggcatccttc acatcgatgc ggttcatgat
     2521 ctcctgggcg cgttcctccg tagaagcccg ggcgctgatc tcgatatcga cggtgatctc
     2581 ctgttggtcc caggtggaga ccacaacatt cccgaattgg ttgtctatct ctaatttgtc
     2641 atcggccgtc accgcatagt tccggtggat cattttctgc ttccttacgt cttcatcgcc
     2701 attggcggct agtgtcatct tcggagcaga taagacaagg agcacggctg ccaggttaag
     2761 tagtcttata tgctttttca tattctgtct tttttgaatg gttgatttgt ttgataatgt
     2821 cgagttgatg attcagcaat cccatctgca gttgaagatt ctgtaacatc gcctcgagca
     2881 gttgttcgcg attggggttt tggggtaact gattttgcag actgtggtaa acactgtcca
     2941 gtttgctgac atcggaagcg aactgccggt acagcagcgg ctcgtctttc tcgatggctt
     3001 tcaactgatt atgttttatc tcgacaagcc gtgcatagtg atacatttct tcgttcatgg
     3061 agttgtcgag ctccgcggtc tctttctcat tcttgtcatc ctgggggcgg atcgaagcga
     3121 gggtaggctc cttggccgct tgggtgctat ccttcaccgg cgcctgcgcc acgggattgg
     3181 ttgccttgct gccttctccg gcaggggtgg tggttgccgg ggccgtattc gtcctgacga
     3241 tggtctgcgg cccttctccc gtccgggatt tgaaatacca tacccctccc gcgatcaata
     3301 ggacgacggc tgcggcggcg ctccatctcc tccagtcagg tctaaccagg ggggcctgtt
     3361 tttttttccc ggggtccaca tccttcagga tcttctccag gaccttcgga tcgggaatct
     3421 ccgcatcgaa ttcttcccgg tgatcctgga caaattgttc taaccggctg ttcatgatac
     3481 tccttgtttt aaatactgta acaatttttg tttggcacgc atgtactggg ttctcaccgt
     3541 ggcatgcgat acctgcagga tctctgcaat ctcttcctgg tcatatcctt caaaaagata
     3601 gagggtcaat accgttctgt acccgttcgg caataatcct accgctttct tgatatcctc
     3661 taccttcaac gctatctcgt tctcctccgg gccttcatcg tccggcagat gtccgatatt
     3721 ggtcttttcg atgtcgatca ggtccatctt ccgtttgcgc aattggttga tggatttatt
     3781 gatcacgatc cgttttagcc acgcgccgaa agtggattta tagtggaagt cttccaggct
     3841 gcggaaggcg tccgtgaagg cctcctgcaa aacatcctcc gcatctcccg tatggttgac
     3901 gatgcgcaaa ctggtattgt acatcgccct ggaatatttc tggtatagtt caccgaaagc
     3961 ccgggagtct ccccgcttgc agcgctccac cagttcatcg tgtagtattg gttccgttaa
     4021 ttccaattca gctattttag cgttagcgtt cattgtacat taaagtacac gagcatttta
     4081 catatgttgc acgaacacct aaaaaacagg catttttctt acattcgcaa gacaatcttg
     4141 ttataaacag ctcgccatgc ccatcacctc gagttcaaaa atagatgttt ttcaaagccc
     4201 tgattactac gagttggacg aattgttgac ggaagtgcac aaaatggtcc gcgattcggt
     4261 tcgcaactat gtcaaaaagg agatctcgcc catcatcgag gattgtgccc aaaaggccga
     4321 attcccgcag cagatcgtcc cgcaactggg agaattgggg tgttttggcc ctaccatccc
     4381 ggagcagtat ggcggcgggg ggctggatta tatcagctat gggctgatga tgcaggagct
     4441 cgaacgcggg gacagcgggg tccggtcgac agcctccgtc cagggttccc tggtgatgtt
     4501 ccccatctac gaatacggca gcgaggaaca gcgccggaaa tacctgccca aactggcctc
     4561 gggggaatgg cttggctgtt ttggtctcac cgagcccaac catggcagcg accccggcgg
     4621 aatgttgaca aatatcaaag atgcggggga tcactatgtc ctgaacggca gcaaaatgtg
     4681 gatcagcaac gccccgtttt cccaggtggc cgtggtctgg gcaaaggacg aggaaggagt
     4741 tattcgcgga gtgatcgtcg aacggggcat ggaaggcttt tccacaccca ccacccatgg
     4801 caaatggagc ctccgcgctt ccgccaccgg agaactggta tttgataatg taaaggttcc
     4861 caaagagaac ctgttaccca acgtaaaagg cctcaaaggc cccctcggct gcctgaccaa
     4921 ggcccgctac ggtatcgcct ggggtgtggt cggcgccgcc atggactgct acgacacggc
     4981 cctccgctat agcctcgaac gggtgcagtt cggccgtccc atcggcggtt tccagctgca
     5041 gcaaaagaaa ctggcggaga tgctcaccga gatcaccaag gcccaattgc tgaactggcg
     5101 cctgggaacc ctgatgaccg ccggaaaggc aacccccgcc caggtatcga tggccaaacg
     5161 caacggctgc gccatcgccg ccgatatcgc ccgcgacgct cgccagatcc tgggcggcat
     5221 gggcatcacc ggcgaatacc cgatcatgcg ccatatgatg aatatcgaaa gcgtcatcac
     5281 ctatgagggt acccatgacg tgcatttact catcaccggg atggacatca ccgggttaaa
     5341 cgcatttaaa tgaagatctt tctcatcggt ttcatgggct cgggtaaaac acactggggc
     5401 cagcgcctgg cagaaaagct gcagctgccc ttctacgatc tggacagcct gatcatcgaa
     5461 aaagagaaca agtcggtggc cgggatcttc gaggagaacg gcgaggaata cttccgcttc
     5521 ctggaaaagg agaccctcga gtcgaccacc gaccagcagg aaagctttat cctctcctgc
     5581 ggcggcggca ccccctgttt ctttaataat atcgaattca tgaagaagaa cggcaaggtc
     5641 gtctggctca acaccgccgt cgacgtgctc cgccagcgcc tgcaacggga aaggatgagc
     5701 cggccgctca tcgccgatgt caccgaggac gacctccagc gctatatcat ccggaaactg
     5761 agcgaacgcc gcatgtacta ccagcaggcc gatgtcacgg tcagcgaaga gcataccaac
     5821 ctggacgaac tgatccgcat cctcctccaa aacgaacaac ccgaccggtt aaaagaagaa
     5881 ctatgaaccg taattttctt tccgtcgccg ccatcctcgg cgcgctttcc gtcgccctcg
     5941 gcgccttcgc cgcccataag ctgaagacaa tggtcggccc ggacgcggta gcgatcttcg
     6001 aaacaggcgt ccgctaccag ttctaccacg tcttcgccct gctctttgtc gcctttctct
     6061 ccgaaaagat ccataacaaa tggatgatct gggccggcaa ctgctttatc atgggcatca
     6121 tcttcttctg cggctccctc tatgtcctca cggccctcac catcgccgag aatacccacc
     6181 gcaccctcgc aggtatcgct accccgatcg gcggcgtctt ctttatcgcc ggctgggtgt
     6241 tcctgtacct gggggtcaag agccgaccgt agggaggctg gccgaaggcc cgacattagc
     6301 ccttcacaaa caggctgacc gcaggctagc cgggaaaccc gcaggctata attgcagata
     6361 gaaagtcgtc cgcgtcccct cctggttctc gatctggaac ccgccttcga tggaggccat
     6421 ccgcttccgc atattgttga ggccattccc aaacttccgc agcttctcca tattgatccc
     6481 gatcccatca tcctggatct cgatgatcaa ccgctcattc accgagatat tgatcttcac
     6541 caccgaactc tgcgaatgct tgagcacatt gttgagcgcc tccttcacgg acaggaatat
     6601 attccgccgt ttctccccgc tgatctcccg cggcgggatc gaggccggca tggtaaaata
     6661 acagtcgatg gccgtattct cgaagaactc aaccgcatag ctgcggatat aggcgataag
     6721 gttctccaca ctatcattac tgctcgtcat cgtccagatg atggtattca tcttgttgat
     6781 cagctcattg gccgaattgg agatcttgtc gatctccggc agggtctgat ccttcatctt
     6841 gctcttgacg atctcgctca tcagccggat ggccgttacc ccggaaccca gctcatcatg
     6901 catgtccacc gagatgcggt tccgctcgtc ctgcttcgcc gccagcacgg ctacctgctt
     6961 ttccatctcc ttccgctcgg tctccagctt catcttttcc tccttctgga tgctgacgat
     7021 gatctccatc ttgttcttat aggtcagcgc aaaaaggaag aacagcgtct cgatggtgat
     7081 gcccacctcg taatggaaca gcgaggcatt gagtacggat accccgctcg cgatcttgat
     7141 gttcgtcgag atcatcacca gggagataat ggacaacaga caaagcgaga tattgcccat
     7201 cacgatatag ttcatcagcc ggttgtgata gctgagcccc ttgatgataa aaatgatgga
     7261 agtagccagc aggaccagct tgatggcggt ctccaggttg ttggcgatgc ggaaatcagc
     7321 ggaaaagaaa taggacagga catacaggac ggtaagcccc gcgatcccca ccgatcccca
     7381 gaacaggtag cggtccaatg gcttgtaatg cttgctcgtc tccaggaact tgcggtgaaa
     7441 gatgatataa aagatatacc ctacggaaaa aatgatattg tccaggaaac tctcgaagaa
     7501 aaaattgaac gaggtagtta cctggtgcag cgtggattta agaaaaagca gcacgctgat
     7561 acagatggaa tagcctatat aataaaggaa ctccggtttc ttgctctgga tatactcgct
     7621 cacggaataa aagatcatca tcagcatgat cccggcgaag agattggtga tcacatcgat
     7681 gctcgtcttg ttcttatgct gaagggtgat accctcctcg atcagcttag cccgggtaag
     7741 ggagggattg atggagttgg ttgccgccct gacataggag agggtggcaa aataggtagc
     7801 gctgtcatgg gccgccagcg tgatcttccg gtagccaaga ctgtcagggt tatcgggcac
     7861 cacccgcggc agggcctcgg catgcatacc ctccggaccg ccgggcacca gccggtacaa
     7921 ttggatcgta tcgacaagcc agccggggca gaagaaaacg ctttgcaccg aatcactgcg
     7981 gttgacgagc gtaaaccgca gcacgggctt tttgttcatc agctccggcg ccataaaatg
     8041 catgtatttc cgggtatttt gccggtaggg tagggaaggc agttgccgga tggaaatatt
     8101 ccggtcgttg gagaaataat acccggtatt gacggcgata tcgtccaaaa ggccggtctg
     8161 ggaaatatcg gccgcaacct ccggctggga ttgctgagcc tggctatcaa gccgcaacaa
     8221 aaggaggaat agaccggaca agcatgcttt taggatattc acagcgcctt tttttaaagg
     8281 taaagtaaat aaattttaat attgcctcgc gtaattgcgt cacttatctt tgtatccatg
     8341 gcaaaccgac agaagtcaaa aaagagccag aagtcctcaa acaagctgaa ggcggaaaaa
     8401 gaagcccagg taacggttgg gcaacttgtc aaagacgaac gtacccacaa gatcaccggg
     8461 gccgtttttc tcctgctggc catcttcctg tttattgctt tcacctctta tcttttcacc
     8521 tggaaggaag accaggacaa ggtctttcat catggcgcct ccgtccttct cccttcctcc
     8581 aacgtgaagg tcagcaacct cctgggcaac ctcggcgcac tggtgtcgca tgaattcttc
     8641 ttcaaaggat ttggcctggc ctcctatttc ttctgctcca tcttctttat catcggggtc
     8701 aacctgctct ttgcccggaa gatcttttcc atcagccgca acctccggta tgtgctggcg
     8761 ggattgctct atttctccgt ggccgccgcc tttatcacta atggcatgga tttcccctgg
     8821 ggcggcgccg tgggcgatat gatcagcgaa tggcttaccc gcttcctggg ctggatcggt
     8881 accgccgccc tcctgctggt ggcggggctg gcctatatta tctggcgctt caacccggta
     8941 ttcaaagttc cttccctgcc gaagaaagag aacgccgccg ttcccgtccc cgagcccctt
     9001 acggaagaaa agcccgagcc aaaggaaaag gagaaggaga agggaaagaa aggagccaaa
     9061 ttattcgtcg acgagtcctt taacaataag gggaatgtat taaaagacga caccggcgtc
     9121 gtggccatcg cgccgccgcc catcgaagaa gagcaggaga tagaattcac cgtcaccgaa
     9181 agggaacccg aagaagagcc gttctccctc atcggcgccc ccctcgaaga agaagaggag
     9241 gaggacgacc tcgacgagga gccggccgag gaacccgcgc cgccgctgaa gatcccgccg
     9301 atccccccca tcgcaaagac cccgctcccc ggcgacctcc aactcgagat aaagaccgta
     9361 ccccccctcg aagaacccgt catggaaggc gactcgctcc tgccttccga cctgccggac
     9421 tatgaaccga cgctcgacct ccgcgactat aaatatcccg gtctcgatct cctcgaagcg
     9481 cacggcagcg aaaagatcgt ccaggacccc tccgaactgg aagcgaataa gaaccagatc
     9541 atcaacaccc tcaagaacta cgatatctcc atctccaaga tcagcgcgac ggtgggaccc
     9601 accgtcaccc tgtacgagat cgtaccggcc gcgggcgtcc gcatctccag gatcaagaac
     9661 ctggaagacg atattgcgct cagcctggcc gccctgggca tccggatcat cgcgccgatc
     9721 ccgggtaagg ggaccatcgg tatcgaggtt cccaatgtga agaagaccgt cgtcagcatg
     9781 cgcaccctgc tgtcttccga caaattccag cacagcaatt tcaacctgcc catcgccatc
     9841 ggcaaaaaga tcgacaacga gaatttcatc gtcgacctcg cctccatgcc gcacctgctg
     9901 atggccggcg ccaccggcca ggggaagtcc gtcggcctca acgccatcct cgtctcgctg
     9961 ctgtacaaga agcacccctc tcagctgaag ctggtactgg tggaccccaa aaaggtggaa
    10021 ctaagcatct accgcaccat cgaaaaacac ttcctggcca agctgccggg cgaagaagag
    10081 gccatcatca ccgacacaaa aaaggtgatc cataccctca acgccctgtg tatcgagatg
    10141 gacaaccgct acgatctgct gaaagaggcc aatgcccgca acataaaaga gtataacgaa
    10201 aagttcatca aacggcggct caacccgcag aaaggccacc aatacctgcc cttcatcgtc
    10261 ctcgtggtgg acgaattcgc cgatctgatc atgacggcag gcaaggaagt ggaaatgccc
    10321 atcgcccgcc tcgcccagct ggcccgtgcg gtcggcatcc acctgatcat cgccacccag
    10381 cgccccagcg tcaatatcat caccggcacc atcaaggcca acttccccgc ccgtatcggg
    10441 ttcaaggtct cttccaagat cgactcccgc accatcctcg acgccggcgg cgccgaccag
    10501 ctcatcggga aaggcgacat gctcatctcc tataacggcg agatcacccg gcttcagtgc
    10561 gcctttgtcg acacgccgga agtcgaagag atcacccagt tcatcggcaa acaaaggggg
    10621 tatgcggagg catacaaatt gcctgaatac gtcgatgaga aagagatgga ggcaaaggat
    10681 ttcgatcttg gcgagcggga ccccctcttt gacgaggcgg caagactgat cgtcgccaac
    10741 cagctgggct ccacctcgct tatccagcgc aaaatgaaac tgggatacaa ccgcgccgga
    10801 aggctcatgg atcagctcga agccgccggc gtcgtaggac ccggccaggg cagcaaaccc
    10861 cgggacgtgc tgataaagac cgagatggaa ttggatcaat atcttaatcc ttagccacta
    10921 agccgccggg gcggctgttc cgaaggacgg acattagcgt ttttaaacta tcttcgcggc
    10981 gcgaagtcaa tagactgtca tacataaaaa aataatcgtg aatagaaaaa ccttcctttt
    11041 ttgcctcgtt ctcggcgcgt tcggtaccct cgctcatgcc caaaacaaca gcctcggtaa
    11101 gaatgacccc gatgccaaaa aggtgctcga cggactcagc acaaagctga aatccttcaa
    11161 agccgtacaa tgcggcttca ccctcacggt aaacgatgca aaggacaagg ttctcggctc
    11221 caagaacgga acgatccaga tcaaggggaa taaatatcat gtcagcgtcg tcggcggaca
    11281 agacatcttc tgcgatggaa aggatatctg gacatacgac aagtcctcca acgaggtcac
    11341 catcaccaag tcggacccca ccacgcaaac catctccatc gataagatct ttaccaattt
    11401 ctacgacaag gactttcttt ataagctcaa tggcgagtca aaggccggcg cccgcaccct
    11461 gcaggaagtg gagctcacgc caaccgacaa aacaaaacca ttcttcaagg ccctgctgta
    11521 tatcgacaag acccagcata cactggccag catcaaatgg tttgataaaa gcggcagcaa
    11581 gtacacgctg acaacaaaga acctcaacgg caacgccgag gtcaccgatg cccagttggc
    11641 atacaataaa gccaaattcc ccggcgcgga agaggtcgat ctgcgcaact aagttaagtt
    11701 ccccagcgga aggttgagat atccaggccg gcgagatcca gcacccggtc cactaccgtg
    11761 gcggccacct cgtcgatggt ctttggaagg ctgtaaaagg agggcgtggc aggacaaata
    11821 atccctccgg ccagtgtcac cgtctccata ttccggatat gtatcaggtt atagggcgta
    11881 tcccgtacca cacagatcag ctttcgccgc tcttttaata cgacatccgc cgcccgggtg
    11941 atcaggtcgg aagaaactcc cgtcgcgatc ctccccagtg tacccatgct gcagggtacg
    12001 atgatcatcg
//
LOCUS       DATGFG010000165         4247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1571410, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000165 DATGFG010000000
VERSION     DATGFG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571410"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..647
                     /gene="frr"
                     /locus_tag="VKQ52_03105"
     CDS             84..647
                     /gene="frr"
                     /locus_tag="VKQ52_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HLZ86197.1"
                     /translation="MSDDLELILEDTRDSMQKALNHLESELVKIRAGKASPQMLEGLT
                     VDYYGTPTVLNQVGNISVMDARTLTIQPWEKNMLQPIERAIINANLGVTPQNDGNIIR
                     LFMPPLTEERRKEFVKRAGNEGEQSRIAIRNIRREAIEQIKKLQKDGLSEDEAKDAEK
                     EIQDITDKHISLVEKHLASKEKEIMSV"
     gene            790..1164
                     /gene="mscL"
                     /locus_tag="VKQ52_03110"
     CDS             790..1164
                     /gene="mscL"
                     /locus_tag="VKQ52_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HLZ86198.1"
                     /translation="MGMLKEFKDFVMRGNVLDLAVAVVIGAAFGAIVSSLVEHIITPL
                     LLTPALKIAHLEDIDALKLGAIKWGSFVSAVIKFVIIAFILFMIVKFFKSLEKKKVPP
                     ASEPTSTEKLLAQILEELKKRP"
     gene            1240..2256
                     /locus_tag="VKQ52_03115"
     CDS             1240..2256
                     /locus_tag="VKQ52_03115"
                     /inference="COORDINATES: protein motif:HMM:NF022720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HLZ86199.1"
                     /translation="MLKNCARKLPILLLITIGLTPIAHAQNGAHSLKSATEAAVITKD
                     PNDTTKMTWKTGGLMSLTFNQAALSNWSAGGDKSALSLNSMVNLYAFYLDGRRSWDNF
                     LNMQYGIANTTSLGTRKTNDIFNVTSKYGYDLGNKWYLSGLLDFRTQFAPGYNYPDAK
                     TRVMTSDFLAPAYLLLSLGMDYKPNNNFSLFLSPATIRELIVNNDSLASVGAFGVDSG
                     KKSRFEYGAYASVNYSSNLSKTAVYTGRLDLFSDYLNHPQNIAMYMTNVLTVKVTSII
                     SMNLAVTLIYDDRIKSVKADGTPGGPALQLQEVIGIGVAYKFAKKARKPTPPPTVPPA
                     PPAQ"
     gene            2315..3205
                     /locus_tag="VKQ52_03120"
     CDS             2315..3205
                     /locus_tag="VKQ52_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HLZ86200.1"
                     /translation="MTNYQIKLPQFEGPFDLLLFFIERDELDIYNIPIHKIIQDFLAY
                     IHGGEALNIELSSEFILFVSTLMRIKAKMLLPRKELDAEGNEIDPRQELIDKLLEYKR
                     YKEASATLAEMEAIRHLMVRRGNLQKELSLIGEEAGEGTEIQAITLFKLMKTFERLMQ
                     KIQQRQNKPVHTVVQYNYTMENSRKYILTAIRQERNLSFERLFDICNDRLHAIFLFLS
                     MLELVQLNYMNILIGEGKNNFIVEFNPDRPEDPLGDAALTGETEPGPDDEGPANGNGV
                     GGGRGDHGGLFPGFLDASAN"
     gene            3213..3926
                     /locus_tag="VKQ52_03125"
     CDS             3213..3926
                     /locus_tag="VKQ52_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HLZ86201.1"
                     /translation="METIYHPSAERGHVNFGWLDSHHSFSFGNWHDREKVQFGALRVL
                     NDDVVKGGMGFDEHPHENMEIISIPLKGALSHKDSTGTEGIIYTGDVQIMSAGSGISH
                     SEYNASHYDAANYLQIWIFPKHPGIRPRHDKRSFHPDDRRDKWQIVVSPRAEDHALWI
                     NQDARLALTRLGAGKSLSYHPAFPGNGIYLFVLEGNITTGGRTLEKRDGFGIAGVPQA
                     DLTALSDSEVLAIEVPMFA"
     gene            complement(3958..>4247)
                     /locus_tag="VKQ52_03130"
     CDS             complement(3958..>4247)
                     /locus_tag="VKQ52_03130"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HLZ86202.1"
                     /translation="VDGSGCTSLGRYRVGYKYRGQFGPAYKLYGLDSSNSRAFARNVV
                     LHSFAAVPERETYPRPICNSLGCPMVAPGFLESLRPVIEGSGKPICLWVFE"
BASE COUNT         1112 a         1383 c          936 g          816 t
ORIGIN      
        1 aaaaagactc gatcctggag caaaacaatt gatgttttga tcttttgacg taggtttgca
       61 acattgtcaa ataacaagcg cttatgtcag acgatctgga acttatcctg gaagataccc
      121 gggatagtat gcaaaaggca ctcaatcacc ttgaatctga actagttaag ataagagcag
      181 gaaaggccag cccacagatg ctcgaaggcc ttaccgtcga ttattacggc acccccaccg
      241 tcctgaacca ggtgggtaac atctccgtta tggatgcccg cactctcacc atacaaccct
      301 gggaaaagaa catgctccag cccatcgaaa gagcgatcat caacgccaac ctcggcgtta
      361 ccccccaaaa cgatgggaat atcatccgcc tcttcatgcc cccgctgacg gaagaaagaa
      421 gaaaagagtt cgtaaaacgg gccggcaacg aaggcgaaca atcaaggatc gccatccgca
      481 atatccgccg cgaggccatc gaacagatca aaaaattgca aaaggacggc ctcagcgaag
      541 acgaagcaaa agacgcggaa aaagagatcc aggatatcac cgataaacat atcagcctcg
      601 tggaaaaaca cctggcgtca aaggaaaaag agatcatgtc ggtttaaccc gcagatcctc
      661 cctctgaaac tccctttcaa gtcaacgatc ctcctcaacc tgtcgcaagg gtagggcccc
      721 ggcgtatact cacgggaccc atcgacattc atgttgtaaa aacatcttta ataacaaaaa
      781 acaacaacca tgggaatgct aaaagagttc aaagacttcg ttatgcgcgg caatgtcctc
      841 gatcttgccg tcgcagtagt tatcggtgca gcctttggcg ccatcgtctc gtccttggtc
      901 gaacacatta ttacaccctt attgttgacc cctgccctca agatcgccca cctggaagat
      961 atcgatgccc tgaaattggg cgcgatcaaa tggggaagct tcgtgtccgc tgtcatcaaa
     1021 ttcgtcatca tcgcctttat tctcttcatg atcgtgaaat ttttcaaatc attggaaaag
     1081 aagaaagttc cgcctgccag cgagcctacg tcgaccgaaa aactcctggc ccagatcctc
     1141 gaagaactaa agaaacgccc ttaaataacg atcagcctgt tgccggcctg gcgggctggc
     1201 aacatttttg ttacctctca tcaataaaac tgacgaatta tgttgaagaa ttgtgcccgg
     1261 aaattaccta tcctcctcct tatcacgatc ggccttaccc ctatagccca cgcacagaat
     1321 ggcgctcact cgctcaagag cgctaccgaa gccgccgtca tcaccaaaga cccgaacgat
     1381 accacaaaga tgacctggaa gaccggcggc ctgatgagcc tcaccttcaa ccaggccgcc
     1441 ctcagcaact ggtcggcggg cggcgacaaa tcagccctgt cccttaactc gatggtcaac
     1501 ctctatgctt tttacctgga tggccgccgc tcctgggata acttcctgaa catgcagtac
     1561 gggatcgcca acaccaccag cctcggcacc cgcaagacaa acgacatctt caatgtcacc
     1621 tccaaatatg gctacgatct gggaaataaa tggtacctct ccggcctcct ggacttccgg
     1681 acccagttcg cccccggcta taactacccc gacgccaaga cgcgggtgat gacctcggac
     1741 ttcctcgccc cggcctactt gctcctctcc ctggggatgg actacaaacc caacaacaac
     1801 ttctccctct tcctgtcccc cgccaccatc cgggaactca tcgtcaacaa cgactccctg
     1861 gcttcagtag gcgctttcgg cgtggattcc ggaaaaaagt cccgcttcga atacggcgcc
     1921 tatgcctccg tcaactattc gtccaatctc agcaagaccg ccgtctacac cggcaggctc
     1981 gacctgttct ccgactacct caatcaccct cagaatatcg ccatgtacat gaccaacgtc
     2041 ctcacggtca aggtgacctc catcatcagc atgaacctcg ccgtgaccct gatctatgac
     2101 gatcggatca agtcggtaaa ggccgacggc acccccggcg gcccggccct ccagttacag
     2161 gaggtcatag gcatcggcgt cgcctataaa ttcgcgaaaa aagcccgcaa acccacccca
     2221 ccgcccaccg tgccgcctgc cccaccggca cagtagacaa cttggggaaa acgcagcccc
     2281 cctttctata aaataaacct attttcgttg ggtcatgacc aattatcaga tcaagctgcc
     2341 tcaattcgag ggtcctttcg acctcctgct cttctttatc gaaagagacg aattagatat
     2401 ttataatata cctattcata agatcattca ggatttcctc gcttacatcc acggcgggga
     2461 agccctgaac atcgaactgt cgagcgaatt catcctcttc gtatccaccc tgatgcggat
     2521 caaggcaaaa atgctcctgc ctcgcaagga actcgatgcc gaaggcaacg agatcgaccc
     2581 ccgccaggaa ctcatcgaca aactcctcga atacaaacgc tataaggaag cttcagccac
     2641 cctggccgaa atggaggcca tccgccacct catggtacgc cgcggcaacc tccagaaaga
     2701 actctcgctt atcggcgaag aagccggcga aggcaccgag atccaggcga tcaccctctt
     2761 caaactcatg aagaccttcg aacgcctgat gcaaaagatc cagcagcggc agaacaaacc
     2821 cgtgcacacc gtcgtacagt acaactatac gatggagaat agccgtaaat atatccttac
     2881 cgccatccgc caggaacgca acctgtcctt cgaacggctg ttcgatatct gtaacgaccg
     2941 gctgcacgcc atcttcctct ttctctccat gctcgaactc gtgcaattga actatatgaa
     3001 tatcctcatc ggcgaaggca agaacaactt tatcgtcgag ttcaaccccg atcgccccga
     3061 agaccccctc ggcgacgcgg cgctcaccgg tgaaacggaa cccggccccg acgatgaagg
     3121 ccccgcaaac ggtaacggcg ttggtggcgg acgcggcgat cacggcggcc tgttccccgg
     3181 ctttttagac gcatcagcaa attagggcgc tcatggaaac gatatatcat ccctcggccg
     3241 aacgcggcca cgtaaacttc ggctggctcg atagccacca ttccttcagc ttcggtaact
     3301 ggcacgaccg cgaaaaagta cagttcggcg cccttcgggt attgaacgac gatgtggtaa
     3361 aaggcggcat gggtttcgac gaacacccgc acgagaacat ggagatcatc tccatcccgc
     3421 tgaaaggcgc cctttcgcac aaggacagca ccggaacgga agggatcatc tataccggcg
     3481 acgtacagat catgagcgcc ggcagcggca tcagccactc cgaatacaac gcctcccact
     3541 acgacgccgc caactactta cagatctgga tcttcccaaa acaccccggc atccgccccc
     3601 gccacgacaa acgctccttt caccccgacg accgcaggga caaatggcag atcgtcgtaa
     3661 gcccccgcgc agaagaccat gccctctgga tcaaccagga tgcccgcctg gccctcaccc
     3721 gcctcggcgc cggcaagtcc ctcagctatc accccgcctt tcccggcaat ggcatctatc
     3781 tgttcgtact ggaagggaat atcaccaccg gcggccgcac cctcgaaaaa cgcgacggct
     3841 tcggcatcgc cggcgtgccc caggcagacc tcaccgccct ctccgactcg gaagtactcg
     3901 ccatcgaagt gccgatgttc gcctgacgcc caaccgaccc gcagcccgca ccgctaacta
     3961 ttcgaatacc cataaacaaa tgggcttccc cgacccctcg atcacgggcc gcagggattc
     4021 caggaaaccc ggcgctacca tcggacaccc cagactgtta catatcggcc ggggataggt
     4081 ctcccgctcc ggcaccgccg caaacgaatg caacaccaca ttccgcgcaa aagcccggct
     4141 attgctgctg tccagcccat acaacttata agccggcccg aactgccccc ggtatttata
     4201 acctacccgg tatctcccca gcgacgtaca cccactgcca tccacat
//
LOCUS       DATGFG010000166         3666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1454841, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000166 DATGFG010000000
VERSION     DATGFG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454841"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /gene="thrS"
                     /locus_tag="VKQ52_03135"
     CDS             complement(<1..1388)
                     /gene="thrS"
                     /locus_tag="VKQ52_03135"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HLZ86203.1"
                     /translation="MIKITFPDGAVREYESGTTALAVARSISEGLAKKVLAASVNGQV
                     WDATRPITMDASLKLLTWTDTDGKSTFWHSSAHLLAEAVEALYPGVKFWVGPPVDGGG
                     FYYDMDLGGRQIGEDDLRKLEVKMAELAKKNEDYRRKEISKAEAIKYFSDKGDEYKLD
                     LLENLQDGNITFYTQGNFTDLCRGPHIPNTSFIKAVRLTNIAGAYWKGDEKNKQLTRV
                     YGVTFPSQKELDEYLAMIEEAKKRDHRKLGKELGIFTFDEQVGPGLPLWMPNGAVIIE
                     QLEKLAKETEERAGYKRVVTPNIAKESLYITSGHLPYYQDSMFPPMELEGEKYYLKAM
                     NCPHHHKIFGAEQKSYKDLPYRLAEYGTCYRYEQSGELFGLMRVRSLHMNDAHIYCTA
                     EQFAQEFNAVNEMYLKYFKIFGIEKYVMRFSTHDPARLGQKFVNEPELWVQTENMVRE
                     VLKNSGINFIEVP"
     gene            1521..2720
                     /locus_tag="VKQ52_03140"
     CDS             1521..2720
                     /locus_tag="VKQ52_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86204.1"
                     /translation="MRRFYLLFLFITPLLLQAQDVTGIWQGHFRSSGSSSMRSSVFDD
                     RYRFEVQIAQNGKSFEGITYSYLSTFFYGKAAAAGTVNPRTSKVLLQEGKLLEYRNAT
                     GDVCIMTCFLQYSKSGDEEFLEGTYTSMNVRDSSNCGRGTVFLRKVPVSDFYTEPFVA
                     KREKEIAEEDSSKGRDKVAIHKPAAKPPARSGVTANPVHRPATAATTPAKPATKPPAD
                     AKSAPPATARTTPPVHKPATPPRTAPPDRNPPMARATVPKETVRQPGSDSSGMGMGRK
                     FTTIAPRVLLDRENQLVKTIVVHTNEVTLNIYDDGAIDHDTVSVYVDNKIVLDHAMLT
                     DRPLVVTIHLDETNDYHEVVMVAENEGEIPPNTSLMIVKAGDKEYEVRITTTEQKNAT
                     VRFQYAK"
     gene            2728..3570
                     /locus_tag="VKQ52_03145"
     CDS             2728..3570
                     /locus_tag="VKQ52_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86205.1"
                     /translation="MNRLPILLLSLLALCSRAEDLNGIWKGTLTQGPGGCYPSYFLEL
                     QINFSNDRITGRAYDYYDKAHFVKMSFTGRYNAQTHRLVLIEDRVLQSDIPAECAPCM
                     KTYDLNYTRNGDIEELIGDWKGYYSERRLICPPGKIKLQRATASDFPVDVDQSDTLAG
                     LQATLHLQPRGKEVVKTVTVNTPQIKIELYDNAEIDHDTVTVMINNKLLLYRQMLTDR
                     PLTINFNAFPNMPYELVMYADNLGDIPPNTALMMVTAGNQKFEVFLSSTEEKSAAVRF
                     IYKP"
BASE COUNT          825 a         1078 c          949 g          814 t
ORIGIN      
        1 gggacctcga taaaattgat gcccgagttt ttcaacacct cgcgcaccat gttctctgtt
       61 tgcacccaca gctccggttc gttcacgaac ttctgcccga gcctggcggg gtcgtgggtg
      121 ctgaaccgca tcacgtattt ctcgatgccg aagattttga aatacttcag gtacatctcg
      181 ttgacggcat tgaactcctg cgcgaactgc tccgcggtgc agtaaatatg cgcgtcgttc
      241 atatgcaaag accgtacccg catcaggccg aacaattccc cgctttgttc atagcggtag
      301 caggttccgt attccgcgag gcggtagggc aggtctttat aggatttctg ttcggccccg
      361 aagatcttgt gatggtgggg gcagttcatg gctttcaggt agtacttctc cccttccagc
      421 tccatcggcg ggaacatgct gtcctggtag tacggcaggt gaccgctggt tatatacaaa
      481 ctttccttcg caatgttcgg ggtgaccacc cgcttgtacc cggcacgctc ttcggtctcc
      541 ttcgccagct tttccaattg ctcgatgatg acggcgccat tgggcatcca caacgggagg
      601 cccggcccca cctgctcgtc aaaggtgaaa atgcccagct ccttgcccag cttccggtga
      661 tcccgcttct tcgcctcctc gatcatggcc aggtattcat ccagctcctt ctgagaaggg
      721 aaggtgacgc catagacgcg ggtgagctgt ttgttcttct cgtcgccctt ccagtaagcc
      781 cctgcgatat tggtcaaccg cacggccttg atgaaactcg tgttcgggat atgcggcccc
      841 cggcagagat ccgtgaaatt gccctgggta taaaaggtta tatttccatc ctgcaggttc
      901 tccaggaggt ctaatttgta ctcatccccc ttatcgctga agtatttgat ggcttccgcc
      961 ttgctgatct ccttgcgacg gtagtcttcg ttcttttttg ccagctcggc catcttgacc
     1021 tccagtttcc gcagatcatc ctcgccgatc tgccgtccgc ccaggtccat atcatagtag
     1081 aacccgccgc cgtcgacggg cgggcccacc cagaacttaa cgcccgggta cagggcttcc
     1141 accgcctcgg ccagcagatg cgccgaagaa tgccagaagg tggatttccc atccgtatcc
     1201 gtccaggtca gcagtttcag ggacgcatcc atcgtgatgg gacgtgtggc atcccatacc
     1261 tgcccgttta cactcgcggc caatactttc tttgccagtc cttcgctgat ggatctggcg
     1321 actgctaacg ctgtggtgcc gctttcgtat tctctgacgg caccgtccgg aaaagtgatc
     1381 ttgatcataa gggtgcaaat ttaacaaact attactatca tcgtgcaacc tctattttac
     1441 aaatggtaca actcggaaag atttatttac ataatttgcc ggcagggaag cggacgcttc
     1501 ggcgtcccaa atcgtagaat atgcggagat tttacctgtt gtttttgttt atcacgcctc
     1561 tcctgctgca ggctcaggac gttaccggca tctggcaggg ccatttccgg agttccggca
     1621 gttcctccat gcggagttcg gtattcgatg accgttatag attcgaagta cagatcgcgc
     1681 aaaacggaaa atcgttcgag ggcatcacct attcctatct gtccaccttt ttttacggca
     1741 aggccgccgc agcgggtacg gtgaacccac ggacctcgaa ggtcctgctg caggaaggca
     1801 aactgctgga atatcgcaat gccaccggcg atgtctgtat catgacctgt ttcctgcagt
     1861 attcgaagtc gggtgacgaa gagttcctgg agggcaccta taccagcatg aatgtccggg
     1921 attccagcaa ctgcggccgc ggcaccgtct tcctccgcaa ggttcccgtc tcggatttct
     1981 ataccgaacc cttcgtggct aaacgggaaa aggagatcgc cgaagaggac tcctcaaagg
     2041 gtagagacaa ggtcgccatc cataagcccg cggccaaacc cccggcaagg tccggcgtta
     2101 ccgccaaccc ggtgcatcgg cccgcaaccg ccgccaccac acccgcgaag ccggcgacaa
     2161 aaccaccggc cgacgcaaag tcagccccac cggcaacggc caggacgacc ccgccggtgc
     2221 ataaaccagc gacaccgccc cgaacagcgc cacccgacag aaacccgccg atggccaggg
     2281 ccactgtgcc gaaggaaacg gtgcgccagc ccggcagtga cagcagcggt atgggtatgg
     2341 gcaggaagtt cacgaccatt gccccccggg tgctgctcga tcgcgagaac cagttggtga
     2401 agaccattgt cgtacatacc aacgaggtta cgctcaatat ctatgacgac ggcgccatcg
     2461 accatgacac cgtctcggtg tacgttgaca ataaaatagt gcttgaccac gcgatgctca
     2521 ccgaccggcc cctggtcgtc actatccatc tcgatgaaac aaacgattac catgaagtgg
     2581 tgatggtagc ggagaacgag ggggaaattc ctcccaacac ctccctgatg atcgtaaaag
     2641 ccggggataa agaatatgaa gtgcgcatta ctaccaccga acaaaagaat gctacagtga
     2701 gattccaata cgccaaatga caagcatatg aatcgtctcc ccatcttatt gctgtcgtta
     2761 ctggcgctct gctcgagggc ggaggacctg aatggcattt ggaagggaac cctgacccag
     2821 ggaccgggcg gatgttatcc cagctatttc ctcgagctgc agatcaactt ctccaacgac
     2881 cggatcaccg gcagggcata tgactactat gacaaggcgc attttgtaaa gatgagcttt
     2941 acgggccggt acaatgccca gacccaccgg ctcgtgctga tcgaagaccg ggtgctgcag
     3001 tcggatatcc ccgccgaatg cgctccctgt atgaagacct acgatctcaa ttatacccgc
     3061 aatggcgata tcgaggagct gatcggcgac tggaagggat actattcgga aagacgtctc
     3121 atctgcccgc cggggaagat caaactgcag cgggcaaccg cttccgattt cccggtcgac
     3181 gtggatcagt ccgacaccct tgccgggttg caggcgacgc tgcacctgca gccgcgcgga
     3241 aaagaagtgg tgaagacggt gacggtgaac accccgcaga tcaagatcga actctatgac
     3301 aatgcggaga tcgatcacga taccgtgacg gtgatgatca acaacaaact gctcttatac
     3361 cggcaaatgc tgaccgaccg gccgctgacc atcaacttca atgcctttcc caacatgcca
     3421 tacgagctgg tgatgtacgc ggacaacctg ggcgatatcc cgccaaacac cgccctgatg
     3481 atggtgaccg cggggaatca gaaattcgag gtctttctat cttccaccga ggaaaaaagc
     3541 gccgccgtgc ggttcattta caaaccctga ttattgcttt ctggtcagat cgaaggagtc
     3601 taccacctcc ccgcaaccca gcatcttgtt cttgaatacg ggatgtttat tccccaggta
     3661 gggatt
//
LOCUS       DATGFG010000167         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1108962, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000167 DATGFG010000000
VERSION     DATGFG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108962"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VKQ52_03150"
     CDS             complement(<1..751)
                     /locus_tag="VKQ52_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86206.1"
                     /translation="MKRINITLFALMLLGILFSCTKASIDYTQNGNWVGRATFPGSVS
                     GFGVAFTIGEAAWVGTGINPQYPNQRLTAFFKYNPTPIPNPLPSPTYYDSTLGTWSKS
                     ATFPGPARSNAVAFTVAGIGYIGSGVGNDGFTPLADFWSYNQGSNSWTQIDSIHDQSG
                     ISQPRLDAAAWGFDTVGYVLTGTDNYNYFLDVWKYSPATGKWTPKPFLPGNPRSGATT
                     WVYNGKGYIMAGFTNSSNHAINNMAYDFWVFD"
     gene            947..1171
                     /locus_tag="VKQ52_03155"
     CDS             947..1171
                     /locus_tag="VKQ52_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86207.1"
                     /translation="MEQPFCPSWRRFEGFLLVDEKGRLVVFFLSLIHTNKIVTTAAPN
                     IAQIRQKGANCASNADIQTFWPLDRLRKPA"
     gene            1168..2595
                     /locus_tag="VKQ52_03160"
     CDS             1168..2595
                     /locus_tag="VKQ52_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HLZ86208.1"
                     /translation="MTKHKYLRKELLFLLSSSLLALFLLIGCQKQPDLLFGSTYTDVN
                     TGANVVLVDTSTILMSTVLVDSTATSGTGYLMVGAYNDDYLGRVTTRAYMQVVPPSSL
                     PTLDPRADNYDSIGMILFARTGNPYYGDTTVYQTYVVNQIDSLYQFGNNQFAWWSNRS
                     QPLGPDLGSASVRIEPNRPSTGGISNTSQGTGDTVRIPMDPALGQQLYTMIYNKSDTI
                     TNNTIFQNWFHGLCLSSGPNIANLIYGFKDSAVMRIYYRENGVVSIGKYIDFALGNKS
                     FQYNNLRPDYTGKPLNNIIRPTQNPQPPPATLSTAIGHVGYVQTIGGLNVKLTFPNIS
                     SIALRQDYIGLLRATLVVKPVPGSFSTIWRLPPQVGIYTTDQNNLLIAPVAALGTAGA
                     QTGNLTLDYFHPLNTEYIYDVTNFVKFQLTNPSPIATQTGLMLTIPSPANTAAFNRLI
                     IADQSYPIDQRITLNVYYISLYPHQ"
     gene            2615..3880
                     /locus_tag="VKQ52_03165"
     CDS             2615..3880
                     /locus_tag="VKQ52_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86209.1"
                     /translation="MKALILLSLLTVTSLGVMAQNNFSPYSQMGIGDVEDGFYNRTTG
                     LGNTGIAYRSNRFLINNNPAAFSGLANQYFTMETGIRGSFIQYAGTPVTPASTQSGDI
                     TFRRLAMGIKAAKNWGTSIGLVPFSTQNYEFNVPYYVQGSSSEVANHYYQGHGSVNKA
                     YWANAYEFFHHISLGVDAGYIFGQLNQKDILQNSNTGASETSTTNDVNLQNLYMTYGM
                     QVYGNLGKHWSYIIGGTYSQRADLLASTTKQVLGSDSSILQNEQLSEGYLTLPNSYGA
                     GISITHNDKYTWLADYRYQDWNSVQRKNSYPGQDYSIASSERGSFGFEISKKRNFYNS
                     KVELSYFQTGVYYGNSYLQINGKQISDMGVTVGFGINSLKSPLAYSVVFQYGIKGTTN
                     NNLIRENYANITFVINYGSIWYTKGKKFD"
BASE COUNT          980 a         1168 c          927 g          847 t
ORIGIN      
        1 gatcgaatac ccagaaatca taggccatat tgttgatggc gtggttgctg ctattggtaa
       61 agccggccat gatatacccc tttccattgt acacccaggt ggtggcgccg gaacgggggt
      121 tgccgggaag gaagggtttc ggcgtccatt tgccggttgc cgggctgtat ttccagacat
      181 cgaggaagta gttatagttg tccgtaccgg tgagtacata gcctaccgtg tcgaaacccc
      241 aggccgcggc atccagccgg ggctgagata tgccggattg gtcatggatg gaatcgatct
      301 gcgtccagga attggaaccc tgattgtagg accagaagtc ggccagcggc gtaaaaccgt
      361 cgttaccgac acccgaaccg atatacccga tgcccgctac ggtaaaagcc acggcattgc
      421 tgcgggcagg gccggggaag gtagccgact tggaccaggt gccaagcgta ctatcataat
      481 aggtaggaga aggaagcgga ttgggaatcg gcgtagggtt atatttaaag aaagcggtca
      541 gacgttgatt gggatattgc ggattgatac ccgtaccgac ccaggcggct tcgccgatgg
      601 taaaggcaac gccaaaacca ctgaccgatc cggggaaggt ggcacggcca acccagttac
      661 cgttttgtgt atagtcgatg ctagctttgg tgcaggagaa caggatacct agtaacatta
      721 acgcgaacaa agtaatgttg atccgcttca taattttttt gatattttgc gctaaagtat
      781 tctaagtcga catagcaaat tcgttaaaat gtattttcag ctggatttaa tagacgaaac
      841 gttaaaattc atatacgaag aaagaatccg cgcctgtttt tccccttgcc gtttttgtta
      901 acgccagcgc tcatctatct ttctgaaaaa gaacaattaa caaagcgtgg aacaaccatt
      961 ttgcccatcc tggcggcgct ttgagggttt tctgctggtc gatgaaaaag gtcgattggt
     1021 cgtttttttc ctatctttga tacatactaa caaaatagta acaactgccg cccctaatat
     1081 tgcacaaatt cgccaaaaag gcgcaaattg cgcctccaat gccgacattc aaacattctg
     1141 gcctttggat cgattaagaa aaccagcatg actaaacata aatatctgag aaaagaactg
     1201 ctttttcttt tgtcctcttc cctattggcc ctgttcttac ttattggctg tcaaaaacaa
     1261 cccgacctgc tgtttggcag cacctatacg gatgtgaaca ccggcgccaa tgtcgtactc
     1321 gtcgatacct ccaccatcct catgtctacc gtcctggtcg actcgacggc tacctccggc
     1381 accggctatc tcatggtggg cgcctataat gacgactacc tgggtagagt gaccacccgg
     1441 gcctatatgc aggtggtccc cccctccagc ctgccgaccc tcgacccgag ggcggataac
     1501 tacgactcga tcgggatgat cctgtttgcc aggaccggta atccttacta tggtgatacc
     1561 accgtttacc agacctatgt ggtcaaccag atagattcgc tgtaccagtt cggcaacaac
     1621 cagttcgcct ggtggagcaa ccggagccag cccctcggac ccgacctggg ttccgccagc
     1681 gtgcggatcg agcccaaccg cccatccacc gggggtatct ccaataccag ccagggcacg
     1741 ggcgataccg tccgcatccc catggaccct gcactgggcc agcagctcta tacgatgatc
     1801 tacaacaagt ccgacacgat caccaacaac accatattcc agaactggtt ccacgggctt
     1861 tgtctctcct cgggacccaa tatcgccaac ctgatctacg gattcaagga cagcgccgtc
     1921 atgcgcatct actaccgcga gaacggcgtc gtcagcatcg ggaaatacat cgacttcgcc
     1981 ctcggcaaca agagcttcca gtacaacaac ctgcggccgg actacaccgg caagcctctc
     2041 aataatataa taaggccgac ccaaaacccg cagcccccgc cggccaccct gtcgacggcg
     2101 atcggccatg taggctatgt gcagaccatc ggcgggctga acgtcaaact caccttcccc
     2161 aatatcagct cgatagccct gcgacaggac tatatcgggc tgctgcgggc gaccctggtc
     2221 gtaaaacccg tgcccggcag cttttcgacc atctggcgtc tgccgccgca ggtagggatc
     2281 tacaccaccg accagaacaa ccttctcatc gcccccgttg ctgccctcgg gacagccggc
     2341 gcacagaccg gtaacctgac gctggattac ttccaccccc tgaatacgga atacatctat
     2401 gatgtcacca atttcgtgaa gttccagctc accaatccaa gccccatcgc aacgcaaacc
     2461 gggttgatgc tcaccattcc gtcaccggcc aataccgcag ccttcaaccg gctgatcatc
     2521 gccgaccagt cctaccctat cgaccagcgc atcaccctga atgtttatta tatttcgcta
     2581 tacccccatc aataaacaaa tgatacctaa tcggatgaaa gctttgattc tcttgtcgct
     2641 gctcacggta acatcccttg gggtgatggc ccagaataac ttttccccct attcccagat
     2701 gggtatcggg gacgtggagg acggcttcta taaccgcacc acgggcctcg gcaatacggg
     2761 aatcgcctac aggagcaacc gcttccttat caataacaat cccgccgcct tctccgggct
     2821 ggccaaccag tactttacga tggagaccgg catccgcggg tcatttatcc aatatgccgg
     2881 cacccccgtt accccggcca gcacccaatc gggcgacatc accttccgca ggctcgccat
     2941 gggcatcaaa gcggcaaaga actgggggac cagcatcggc ctggtgccct tcagcaccca
     3001 gaactacgag ttcaatgtcc cctattatgt ccagggtagc agctcggaag tggccaacca
     3061 ctattaccag gggcacggta gcgtcaataa agcttactgg gcaaacgcct acgagttctt
     3121 ccatcatatc tccctcgggg tcgacgccgg ctatatcttc ggccagctga accagaagga
     3181 catcctgcag aacagcaaca ccggcgcctc cgagacctcg accaccaacg acgtcaatct
     3241 gcagaacctg tatatgacct acggcatgca ggtctatggg aatctgggca aacactggtc
     3301 ctatatcatc ggcggaacct attcccaacg agccgacctg ctggcctcca ctactaaaca
     3361 ggtgctgggc agcgactctt ccatcctgca gaacgaacag ctctccgagg gatacctgac
     3421 cctgcccaac agctacggcg ccggcatctc catcacccat aacgacaagt atacctggct
     3481 cgcggactac cgctaccagg actggaacag cgtccagcgc aagaacagct atcccggcca
     3541 ggactacagc atcgccagca gcgaacgcgg ctccttcggt ttcgagatct ccaaaaaacg
     3601 aaatttctac aacagcaagg tagagctcag ctatttccag accggggtct actatggcaa
     3661 ttcctatctc cagatcaatg gcaaacagat ctccgatatg ggtgtgacag tgggctttgg
     3721 gatcaactcc ctgaaatccc ccctcgccta ttccgtcgtc ttccagtacg ggataaaggg
     3781 caccaccaac aacaacctga tccgggagaa ctatgcaaat attacttttg ttattaacta
     3841 cggctccatc tggtatacaa aaggaaagaa atttgactaa tatgcttgat ctccaccagc
     3901 agatgaaagg cttccccctc at
//
LOCUS       DATGFG010000168         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1108947, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000168 DATGFG010000000
VERSION     DATGFG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108947"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VKQ52_03170"
     CDS             complement(<1..717)
                     /locus_tag="VKQ52_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86210.1"
                     /translation="MNVTSTNYQRHKLLIAGFLFIYTLSIFLPGAYSVDSWNQWKEVT
                     SKHYDDWYGTGLATSWRFLWVLTGNYMCLFVAQMLLYWIFITLIFWEVPFKSPVYWLG
                     LGAGLFFCLIPQYVMRDSLTVLAWGVAALLLLYAGRSETHRRSLAIAGLLLLAYGVWI
                     RINAFIAFLPLAYAGILLLSRRRIAPWKGMLLSGAACLLLMVTIQLFTYRVQKAERLY
                     PDYKLKLLDLSGISKLSGENC"
     gene            complement(720..>2513)
                     /gene="uvrA"
                     /locus_tag="VKQ52_03175"
     CDS             complement(720..>2513)
                     /gene="uvrA"
                     /locus_tag="VKQ52_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HLZ86211.1"
                     /translation="QFVKGAKKFDFPIHKPVIDLNKQQYKTLWEGNEHVQGINDFFAE
                     VERNLYKVQYRVLLSRYRGRTECPDCKGYRLRKEALYVRVGDRHIGELCGMPVKDLVD
                     WFAQLELSLHQQQIAKRILIEIHHRLRTLMDVGLGYLTMARLANSLSGGESQRIQLTR
                     SLGSNLTSSLYILDEPSIGLHSRDTERLIRVLKELRDLGNTVVVVEHDEMMMREADYI
                     IDMGPLASHLGGEVVAAGDYQALINDEDSLTGKYLKGELTITPPATVRKWNRSLILEG
                     ARQNNLQDLTVEFPLGILTVVSGVSGSGKTTLVKQILYPALQKIKGEPAEKVGLHKAL
                     KGDIDSISQVEMVDQNPIGKSSRSNPVTYIKAYDEIRDLYSRQPLSKMRGFQPKHFSF
                     NVDGGRCDTCKGEGEQVVEMQFLADVHLTCEVCHGKRFKEEILEVHYREKSIYEILEM
                     SVDEALEFFKDEKDIYQKIKPLSDVGLGYVKLGQSSDTLSGGEAQRVKLASFLGKGRA
                     QGHILFIFDEPTTGLHFHDIRKLLASFNALIDQGHSILVIEHNTDVIRSADWVIDLGP
                     EAGDGGGNLVYAGVPAGLKKVKESYTGKFLS"
BASE COUNT          541 a          698 c          710 g          564 t
ORIGIN      
        1 acaattctcc ccgctgagtt tggaaatgcc ggagaggtcc agcagtttta atttgtaatc
       61 gggatagagc cgttccgctt tctgtaccct gtaggtgaac aactggatcg tcaccatcag
      121 caggagacag gcggcgccgg agagcagcat gcctttccag ggagcgatcc gccgccggct
      181 caatagcagg atgcccgcat aggccagggg cagaaaggcg ataaaggcat ttatccggat
      241 ccagacgcca taggccagca gcaacagacc ggcgatggcc aggctcctgc ggtgggtttc
      301 gctcctgccg gcatataaaa gtaagagcgc ggcgactccc caggccagca ccgtgagcga
      361 atcacgcatg acatattgcg ggataaggca aaagaagagg cccgcgccca ggccaagcca
      421 atagacgggg cttttgaacg gcacctccca aaaaataagg gtgatgaata tccagtacag
      481 gagcatctgc gccacaaaca ggcacatata gttgccggta aggacccaca gaaaacgcca
      541 gctggtcgcc agccccgtgc cataccaatc gtcatagtgt ttgctggtca cctccttcca
      601 ctggttccag gaatcgacgc tataagcgcc gggaagaaag atcgacaggg tgtagataaa
      661 caggaacccg gcgatgagta atttatgccg ctggtagtta gtagaggtga cgttcatgct
      721 tagcttagga atttacccgt gtagctttcc ttcaccttct tcagaccggc aggcaccccg
      781 gcatacacca ggtttccacc gccgtcaccg gcttcgggtc ccaggtcgat gacccagtcg
      841 gccgaacgga taacgtcggt gttgtgctcg atgaccagga tggaatgtcc ctggtcgata
      901 agcgcgttga aggaggccag gagcttgcgg atatcatgaa agtgaagccc ggtggtcggt
      961 tcgtcaaaaa taaacaggat atgtccctgc gccctgccct tccccaggaa ggaagcaagc
     1021 ttgacacgct gggcctcgcc gccggaaagg gtgtcggatg actggcccag ctttacatag
     1081 ccgaggccca cgtcactgag gggctttatc ttctggtaga tatccttctc gtccttaaag
     1141 aactccagcg cttcatccac gctcatctcc aatatctcat agatgctctt ttcccggtag
     1201 tgaacctcga ggatctcttc tttgaaccgt ttgccatggc agacctcgca ggtgagatgg
     1261 acatcggcca ggaactgcat ttcgacgacc tgttcgcctt cccccttgca ggtgtcgcat
     1321 ctgccgccgt cgacattaaa ggagaaatgc ttgggctgga agccgcgcat cttactgagc
     1381 ggctggcggg agtagagatc gcggatctcg tcatacgcct ttatatacgt tacaggattg
     1441 ctcctggacg atttacctat cgggttctga tcgaccatct ccacctggga gatcgaatcg
     1501 atatcaccct tcaacgcctt atgcagcccg accttctccg ccggttcgcc ctttatcttt
     1561 tgcagggccg ggtagaggat ctgtttcacc agggtagtct tgccgctgcc actgacgccg
     1621 ctcaccacgg tgaggatgcc gagggggaat tccacggtga ggtcctgcag gttgttttgg
     1681 cgggcacctt cgaggataag cgatcggttc catttccgca cggtggccgg cggcgtgatg
     1741 gtcagctctc ctttcaggta cttgccggtg agactgtctt cgtcgttgat cagggcctgg
     1801 tagtcgccgg cggctaccac ttccccgcca agatgggagg ccaggggacc catatcgatg
     1861 atgtagtcgg cctcgcgcat catcatctca tcgtgctcga ccacaacgac ggtattgccg
     1921 agatcgcgta attcctttaa tacccggatc agcctttccg tatcgcggga gtggagaccg
     1981 atggaaggtt cgtcgagaat gtagagtgaa ctggtgagat tgctgcccag gctgcgtgtc
     2041 aattggatcc gctggctctc gccgccgctg aggctgttgg cgagccgggc catggtcaga
     2101 tatcccagac cgacatccat gagggtccgc agccggtgat ggatctcgat gagaatacgt
     2161 ttggcgatct gttgctggtg cagactcagc tccagctgag cgaaccagtc taccagatcc
     2221 tttacgggca tcccgcagag ctcaccgata tggcgatcgc cgacacggac ataaagcgcc
     2281 tctttccgca ggcggtagcc cttgcaatcc ggacattcgg tgcgtccgcg atagcgggac
     2341 aacaacaccc ggtattgcac tttgtagaga ttgcgttcca cttcggcgaa aaaatcattg
     2401 atcccctgta cgtgttcatt gccttcccag agcgtcttgt attgttgttt gttgagatcg
     2461 atcaccggct tgtggatggg gaagtcgaat ttcttggctc cttttacgaa ttg
//
LOCUS       DATGFG010000169         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_197087, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000169 DATGFG010000000
VERSION     DATGFG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197087"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VKQ52_03180"
     CDS             <1..425
                     /locus_tag="VKQ52_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HLZ86212.1"
                     /translation="YTQHTLLKDTDQMSMAVSLEVREPFFDQDLVEYILAVPDRIKNP
                     VYPKSLLVESVKPLLPDEVVFRKKQGFLFPWEIWLRNELRSFCETRLRNMAQRPFVRG
                     KCLLDYWQRFLAGDKSIRWAELWLFVVLEYWMEVNDVS"
     gene            422..1567
                     /locus_tag="VKQ52_03185"
     CDS             422..1567
                     /locus_tag="VKQ52_03185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ86213.1"
                     /translation="MSTKKKILVLVDWFAPGYRAGGPIQSCVNFAYAMRGNYEVSVLT
                     TDTDHGETAPYPGIPAGRWIDGLHAGFRVNYLPKASLSKGMIRSAMLDADADYVYLNH
                     LFSPLFVVYPLWLKYRGQLRSEVVVCPRGALYDSALSVKPWKKTPFLTLFRLLGIHKK
                     VLFHATNQREKEAIERFFPGSKILIADNLPNTNQPEFVVIPKQPGMLKAVFIARIHPI
                     KNLLFLLQALRAVTATVELTVIGPMEDRSYWESCQAEIARLPAHVRVDYLGPKRNDEL
                     MPVLQAHHLFILPTTGENFGHAIFEALLAGRPVLISDQTPWLGMPAFKAGWDLPLNDV
                     MGFTRVIEEAAGWDEARFGEWARGAWNYARNFIKNPELQNQYYKLFA"
     gene            1564..2127
                     /locus_tag="VKQ52_03190"
     CDS             1564..2127
                     /locus_tag="VKQ52_03190"
                     /inference="COORDINATES: protein motif:HMM:NF007797.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HLZ86214.1"
                     /translation="MNPVLTKKVDNSAYRTTITIGASRGKQMAWYLVNILFFKNPLNI
                     ISSLKVSLLRLFGARLGQGVVVKPAVNIKYPWKLRVGDHTWIGEEVWIDNLSEVVIGS
                     HVTLSQGTLLLTGSHDHTRTTFDFLSQPIILEDGVWIGAKAVVAGGITCRTHSILGMN
                     SVAEKDLEPYTIYKGNPAVPVIIRTIT"
     gene            2124..4070
                     /locus_tag="VKQ52_03195"
     CDS             2124..4070
                     /locus_tag="VKQ52_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLZ86215.1"
                     /translation="MTPDIRTINHKVLKDALRIRKVEEKFLELFSLGKLNGTVHTCVG
                     QEFSALAFAGQLKKKDFVFSNHRCHGHYIAFTGDTRGLLAELLGKRSGTCGGIGSSQH
                     LCHNNFFSNGIQGGIVPVAAGYALGNKLSHNGAIGVVFIGDGTLGEGTLYETMNIISK
                     WEIPLLIVCENNFYAQSTPQHINLAGDIQARAEAFGIRTDRGNTWAPEDLITRAQESI
                     DYVRETSRPCFFLVETYRLNAHSKGDDDRDPAEVEAHREKDFLQLFRKESPSYYQLYN
                     EALDEEINSMVDEILEEGELDLDEYYRQEETVTGDDWVALEAINKRQVELLNAFFREQ
                     IVADERTVFLGEDILSPYGGAFKVTRELSFLRPERVFSTPISESAITGISNGLALNGY
                     KPFMEIMFGDFMTLAFDQIVNHASKFHHMFNKKVNCPIVIRTPMGGRRGYGPTHSQTL
                     DKFLVGIDNVKTVALNTFVDPAILYRAVKDEMHPVIVIENKTDYGKKIAQQQLPHFVY
                     ECNGDAYPVVRIRPAVSAPTLTIVAYGGMSEIVAGLLDAIFMETDHKPELIIPSLISR
                     LPVDLVADAVADTGRLLVVEEGSGYAGIGSELIAAVVERSGVSFTARRVAAYPVPIPS
                     VKSLENSVLPDKNRILAEIKAYLH"
     gene            4070..>4442
                     /locus_tag="VKQ52_03200"
     CDS             4070..>4442
                     /locus_tag="VKQ52_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HLZ86216.1"
                     /translation="MAVIQEIGVPLLAVNDTTLTVVELPFSAGARVKKGDVILVFETS
                     KTSYDVEAETDGFIQYLCEADRDYAVNDVVARIYSEESEALAPAPGGKRGAGAASAAG
                     AGSAGSGVKGGAAGAASVSVAG"
BASE COUNT          924 a         1141 c         1412 g          965 t
ORIGIN      
        1 gctataccca gcatacgctg ctaaaggata ccgatcagat gagcatggcc gttagcctgg
       61 aggtgcggga gcccttcttc gaccaggatc tcgtggaata tatactggcc gtaccggaca
      121 ggatcaagaa cccggtgtac ccgaaaagcc tgctggtgga atcggtgaag cccctgctgc
      181 cggatgaagt ggtcttccgg aaaaagcagg gttttctctt cccctgggag atctggctcc
      241 ggaatgagtt acggtccttt tgcgagaccc gtttgcggaa tatggcccaa cgcccttttg
      301 tccgcgggaa gtgcctgctt gactactggc agcgtttcct ggccggggat aagagcatcc
      361 gctgggcgga attatggttg ttcgtcgtgc tggaatattg gatggaggta aatgacgtct
      421 catgagtacc aagaaaaaga tcctcgtgct tgtggattgg ttcgcccccg gttaccgggc
      481 gggtgggccg atccagtcct gtgtgaactt cgcctatgcc atgcggggaa actatgaggt
      541 gtccgtgctc accaccgata ccgatcacgg cgagacggcg ccctatcccg gcattcccgc
      601 cggccggtgg atcgacggtc tccacgccgg attccgcgtg aactacctgc caaaggcaag
      661 cctgagcaag ggaatgatca ggagtgcgat gctggatgcg gatgccgact atgtctacct
      721 gaaccatttg ttctcgcccc tttttgtcgt ttacccgttg tggttaaaat atcgcgggca
      781 gctccggagt gaagtggtgg tttgtccccg tggcgcgctt tatgacagtg cgctgtcggt
      841 aaaaccctgg aagaagaccc cctttttaac cttgttccgc ctgctgggta ttcataagaa
      901 ggtgcttttt catgccacca accagcggga gaaggaagcc atcgaacggt tctttcccgg
      961 gagtaagatc ctgatcgccg ataacctgcc caataccaac caaccggagt ttgtggtgat
     1021 cccgaagcag ccggggatgt tgaaggcggt gttcattgcg cggatccacc cgataaagaa
     1081 cctgcttttt ttgttgcagg ccttgcgggc ggtgacggca acggtggaat tgacggtgat
     1141 cggtccgatg gaagacaggt cctattggga gtcctgccag gcggagattg cgcggctgcc
     1201 ggcgcatgtc cgtgtcgatt acctggggcc gaagaggaat gatgagctga tgcctgtgtt
     1261 gcaggcgcat cacctgttca tcctgccgac caccggggag aatttcgggc atgccatctt
     1321 cgaagccttg ttagccggcc ggcccgtgct gatcagcgac cagacgccct ggcttgggat
     1381 gccggcattc aaggccggct gggatctgcc gctgaatgat gtgatgggtt ttacccgggt
     1441 gatcgaagag gcggcaggct gggacgaggc ccgtttcggg gaatgggccc ggggcgcctg
     1501 gaattatgcc cggaatttca ttaaaaatcc ggaacttcag aatcaatact ataagctgtt
     1561 tgcatgaacc cggtcctcac gaagaaagtc gacaattccg cttaccggac caccattact
     1621 atcggggctt cgcgggggaa gcaaatggcc tggtacctgg tcaatatcct gttctttaag
     1681 aacccgttga atattatttc ctccctgaag gtgagcctgt tacggctatt tggggcccgg
     1741 cttgggcagg gggtcgtcgt aaaaccggcg gtgaatatca aatacccctg gaagctcagg
     1801 gtcggcgatc atacctggat cggcgaggag gtttggatcg ataacctttc ggaggtggtc
     1861 atcggcagtc atgtcacgct ttcgcaggga acgctgttgc tcaccggcag tcatgaccat
     1921 acgcgcacta cctttgactt cctttcccag ccgatcatcc tcgaggatgg ggtctggatc
     1981 ggggcaaagg cggtggtcgc cggcggcatc acctgcagga cccatagcat cctggggatg
     2041 aactcggttg cggaaaaaga cctggaaccc tatactatct acaagggcaa ccccgcggtg
     2101 cccgtcatca taagaacaat aacttgacac ccgacatacg caccatcaat cataaagtat
     2161 taaaggacgc cttacgcatc cggaaggtgg aggagaaatt cctggaatta ttctccctcg
     2221 ggaagctgaa tggcaccgtc cacacctgcg tcggccagga gttctccgcg ctggcttttg
     2281 cggggcagct gaagaagaag gactttgtct tctccaatca ccggtgtcat gggcattata
     2341 tcgcttttac gggtgacacc cgcggcctgc tggccgagct gctgggaaag cggagcggca
     2401 cctgcggggg catcggcagc agccagcatc tctgtcacaa taatttcttc tcgaacggca
     2461 tccagggcgg gatcgtccct gtcgcggcgg gttatgcgct tggcaacaag ctgagccata
     2521 acggcgccat cggggtcgtg ttcatcggcg acggcactct tggagaaggg acgctgtatg
     2581 agacgatgaa catcatcagc aaatgggaga tccccctgct gatcgtctgt gagaataact
     2641 tctatgcgca gtcgacgccg cagcatatca atcttgccgg ggatatccag gcaagggcgg
     2701 aagcctttgg catccgcacc gatcgcggga atacctgggc gccggaagac ctgatcacga
     2761 gggcccagga gtcgatcgac tatgtccggg agacatcgcg gccctgtttc ttcctggtcg
     2821 agacctaccg gctgaatgcg cactccaagg gggatgatga ccgtgatccc gccgaggtcg
     2881 aggcgcatcg cgaaaaggat tttctccagc tcttccgcaa ggaatcgccc tcctactatc
     2941 aactgtataa cgaggccctg gacgaggaga tcaatagtat ggtggacgag atcctggaag
     3001 agggtgagct ggatttggat gagtattacc ggcaggagga gacggtcacc ggggatgact
     3061 gggtggcgct ggaggcgatc aataagcggc aggtggaatt gctgaatgcc tttttccggg
     3121 agcagatcgt cgcggacgag cggaccgttt tcctcgggga ggacatcctt tccccctatg
     3181 gcggggcctt caaggtgacg cgggaactgt cttttctgcg gccggaacgc gtgttctcga
     3241 cgccaatatc cgagtcggcc atcacgggga tctccaacgg gcttgcgctt aacggctaca
     3301 aacccttcat ggagatcatg tttggggact tcatgacgct ggccttcgac cagatagtca
     3361 atcatgcttc gaagttccat catatgttca acaaaaaggt gaactgtccc atcgttatcc
     3421 ggacgccgat ggggggaaga aggggatatg ggcccacgca ttcccagacc ctcgataagt
     3481 tcctcgtggg gatcgataat gtgaagacgg tggcgctgaa tacgttcgtg gacccggcga
     3541 tcctctatcg cgccgtgaag gacgaaatgc acccggtgat cgttatcgag aataaaacgg
     3601 attatgggaa gaagatcgca caacagcaat tgccccattt cgtttacgag tgcaacgggg
     3661 atgcctaccc ggtggtgcgt atccggccgg cggtgtcggc gccgaccctg accatcgtcg
     3721 cctatggcgg gatgtcggag atcgtggcgg ggctgctgga tgcgatcttt atggagaccg
     3781 atcacaagcc ggaactgatc attccctccc ttatctcgcg gctgcctgta gacctggtgg
     3841 ccgatgcggt ggcggatacg ggccggttgc tggtggtgga ggaaggatcg gggtacgccg
     3901 gcatcggcag tgagctgatc gccgcggtgg tggaacgcag cggggtgtca ttcaccgcgc
     3961 ggcgggttgc ggcgtatccc gtgccgatcc cttcggtaaa atcattggag aacagtgtcc
     4021 tgcccgataa gaacaggata ttggcggaaa taaaagcata tctacactaa tggcagtaat
     4081 acaggaaata ggcgtgccgc tgctggccgt gaacgatacc acgcttacgg tggtggagtt
     4141 gccgttcagc gccggggcaa gggtgaagaa gggggatgtg atcctggtgt tcgagacctc
     4201 gaagaccagt tatgatgtgg aggcggagac ggatggcttt atccagtatc tctgtgaggc
     4261 cgaccgggat tatgctgtaa atgatgtggt ggcgaggatc tatagtgagg agtcggaggc
     4321 gttggctccg gcgccgggag ggaagagggg ggccggggcg gcttcggctg ccggtgcggg
     4381 ttcggcgggt tcgggagtga aggggggggc tgcgggtgcg gcttcggttt cggtggccgg
     4441 tt
//
LOCUS       DATGFG010000170         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_197007, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000170 DATGFG010000000
VERSION     DATGFG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197007"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VKQ52_03205"
     CDS             complement(<1..700)
                     /locus_tag="VKQ52_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86217.1"
                     /translation="MNEYNQRDTTSVSGQDELLRVLIDKVVDNGTAIRETRAEIRRLP
                     EPGAAMEGLDQRIGALEEKVGVIGTGMGERIGGLERQMEVVAKGVAGLGAQVGEPTGW
                     LLSGMRNLQSSLEVYVDFFTKPMQKEVHHRHFLGRPMLLGFVVLFVIGLQWYGLIRSW
                     NREDMYEKNDLLWRGAKLSGDSVVTRALDKVKRDYESDPGQFGKDVVTEEERRAELFE
                     RWRQVNEGMGKIRVL"
     gene            complement(721..1677)
                     /locus_tag="VKQ52_03210"
     CDS             complement(721..1677)
                     /locus_tag="VKQ52_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HLZ86218.1"
                     /translation="MIARTFLTGKGFGETCAYLCQDQSRAQVLAAEGVRTHELRLMAA
                     DFEWQHGLMPEKEKPVYHGVLSFPGEERVNDDRLVELGRRYLEKIGMVNTQYVFVKHT
                     DKEHLHDHILANKVSNDGEPIGKGLIIERGIQAAQELTEEYGLRKEEGKRLERTHQEA
                     LHEPDAKRYRIYQAIQEELPRCVGLEELEKGLLQRGIAVRYRYDVETGEPQGISFRFA
                     NRSFKGSRVDKMCSMKELKQTLQLQEELRQKPGLFEGLEFGERLRLMRDDSLRKKEEL
                     RVEESDRQVALRQEELRQSAELRRVEELREVQGLRRGLRHGF"
     gene            complement(1667..2008)
                     /locus_tag="VKQ52_03215"
     CDS             complement(1667..2008)
                     /locus_tag="VKQ52_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86219.1"
                     /translation="MAKIRGRPKKARKQEKNIGFFVTKLQYFVIQQKAAKARVTISDY
                     MRQMAVNGYVKAKWTEEERQMVKQLIGMSVDLNRLVELAGTQGAMQTALFFAKYRGIM
                     DEIINTLCHDR"
     gene            2244..2867
                     /locus_tag="VKQ52_03220"
     CDS             2244..2867
                     /locus_tag="VKQ52_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLZ86220.1"
                     /translation="MSKTFFDIGLSLDGFIAGENRSPQNPMGGQAAAIHNWMFIQKAF
                     WEHLHTTGGEKDDQDGDLIRNVFARTGAYVMGKRMFDEGEASWPEGLFKAPVYVLTHE
                     KRQPWVQKDNTTFYFVNDGMESAYAKAKASAGNKDIRIQGGADCIRQYLNAGYIDEFT
                     LHIAPSLLGSGLRFFDGVDKTKFSFKIINTIQSPAVTHITYAVTKLK"
BASE COUNT          693 a         1056 c          576 g          650 t
ORIGIN      
        1 ccaacacccg gatcttcccc attccttcat tcacctgcct ccaccgctcg aacaactccg
       61 ccctccgttc ctcctccgtc acaacatcct tcccgaactg ccccggatca ctctcataat
      121 cccgcttcac cttatccaaa gcccgcgtca ccaccgaatc cccggataac ttcgcccccc
      181 gccataaaag atcattcttc tcatacatat cctccctgtt ccatgatcgg atcaacccat
      241 accattgcaa tccaataacg aacaacacca caaaccctag caacatcggc cgcccaagaa
      301 aatgccggtg atgcacctcc ttctgcatcg gttttgtaaa aaaatccaca tacacctcca
      361 ggctgctttg caaattcctc ataccactta gcaaccaccc ggttggttcc cccacctgcg
      421 caccaagccc ggctacgccc ttggcgacaa cctccatctg ccgctccaac ccgccgatcc
      481 gctcacccat tcccgttcct atcacaccta ccttctcttc cagcgcgccg atccgctgat
      541 ccagcccctc cattgccgca cccggctccg gaagccgtcg gatctccgcc cgcgtctccc
      601 ttatcgcggt accgttatca accaccttgt cgatcaatac ccttaataat tcatcttgcc
      661 cggacacact cgttgtgtcc cgttgattgt actcattcat aaaataaagt tttaaattgt
      721 ttaaaaacca tgccgcaacc cccggcgcaa cccctgcacc tcgcgtaact cttccacccg
      781 gcgcaactcc gccgattgtc gtaactcttc ctgccgcaat gccacttgcc gatctgactc
      841 ctcaacccgc aactcctcct tcttcctcaa cgaatcatcc cgcatcaacc gcaaccgctc
      901 cccgaattcc aatccttcga aaagcccggg cttttgccgc aattcttcct gcaactgcag
      961 cgtctgcttc aactccttca tcgagcacat cttatccacg cggctccctt tgaaacttcg
     1021 attcgcgaac cggaaactga tgccctgcgg ctcacccgtc tccacatcat accggtaccg
     1081 gacagcgatc ccccgttgca acaacccctt ctccaactcc tccaaaccaa cacatcgggg
     1141 aagttcctcc tgtatggcct ggtagatccg ataccgcttg gcgtcaggct catgcaacgc
     1201 ctcctggtgt gtccgctcca accgcttccc ctcttcctta cggagaccat actcttcggt
     1261 aagctcctgc gccgcttgta tcccccgctc gatgatcagc cccttcccga tcggctcccc
     1321 gtcattgctg accttgttgg caagaatatg gtcatgcagg tgctccttat cggtatgctt
     1381 gacaaagaca tattgcgtat tgaccatgcc gatcttctcc aggtaccgcc gccccagctc
     1441 tactaacctg tcatcgttca cccgttcctc acccggaaag ctgagcacac catggtacac
     1501 cggcttctcc ttctccggca tcagcccgtg ctgccactcg aaatcagccg ccatcagccg
     1561 cagctcatgg gtcctgaccc cttcagcggc cagcacctgc gcgcgggact ggtcctggca
     1621 gagatacgca caggtctcgc caaagccctt acccgtaaga aatgtcctag cgatcatgac
     1681 ataaggtatt aatgatctca tccataattc cccggtactt cgcaaagaac aaggccgtct
     1741 gcatggcccc ctgcgtcccg gctaactcca ccagccggtt aaggtccacg gacatcccga
     1801 tcaactgctt caccatctgc cgctcttcct ccgtccactt ggcctttacg tacccgttca
     1861 ccgccatctg gcgcatgtaa tcggatatag taacccgggc cttcgcggcc ttctgctgga
     1921 tcacgaaata ctgtagcttc gttacaaaga agccgatatt cttttcctgc ttcctggcct
     1981 tcttaggccg tcctctgatc tttgccattt tttttgcccg taaataacag aaaagaacaa
     2041 tccgcaaaaa ggcaggacgt cctgggaaaa taatcggcta taaatcaagg tgaaaaatgg
     2101 ttaatcctcc agatccggaa ccctgccggg ccagtaacgc tcatggctga ttgctggaat
     2161 ttgtcgcaat ccgccctgct aaatgtcgga cccgggtgtt ttccaaaccc ctgcaacaaa
     2221 ttacctttga aaaaaacaaa accatgagca agacattctt cgacatcggt ctctccctgg
     2281 acggcttcat cgccggcgag aaccgcagcc cccaaaaccc catgggcggt caggccgccg
     2341 ccatccacaa ctggatgttc atccaaaaag cattctggga acacctgcac accaccggcg
     2401 gcgaaaagga cgaccaggac ggcgacctca tccgcaacgt ctttgccaga acgggcgcct
     2461 atgtcatggg caaaagaatg ttcgacgaag gcgaagccag ctggcccgaa gggctgttca
     2521 aagccccggt ctatgtcctc acccacgaaa aacgccaacc ctgggtgcaa aaggacaata
     2581 cgacctttta ttttgtaaat gacggaatgg aaagcgccta tgccaaggca aaagccagcg
     2641 ccggaaacaa ggacatccgc atccagggcg gcgcggactg catccgccaa tacctcaacg
     2701 ccggctatat cgacgaattt accctccaca tcgcccccag cctcctcggc agcggcctcc
     2761 gattcttcga cggcgtggac aaaacaaaat tcagtttcaa gattatcaac actatccagt
     2821 caccggccgt aacccatatc acctatgcgg tcactaagct aaaataaggt gcatcaccgc
     2881 caacggcccg gatgccttaa ctcaacacca acccctcctt caacgcattc tcataacaga
     2941 ccttgtcaaa tcggtagagg tgcgggctcc ggtgc
//
LOCUS       DATGFG010000171         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_196941, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000171 DATGFG010000000
VERSION     DATGFG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196941"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..2896
                     /locus_tag="VKQ52_03225"
     CDS             419..2896
                     /locus_tag="VKQ52_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ86221.1"
                     /translation="MSVNVISLKIAWRNTLRQRWYSLIHVLGLAIGICVCIIIFLIGR
                     YELSFDNFHPDAYRIYRIVGEVEDKDGNLIFLNSPLREEAGIEHLIAGFEAKTAFHIF
                     GSTISVPSPNGQVTKVYDNRQDDSYALSTILTDPDFFRLFPHEWLAGSPQVLDQPGRI
                     VLTASVAGKYFGPGRVEQMLGRKVIYDDSLTLTVAGIVRDWNRPSDLNYTSFISIKTA
                     PATWLRERIPTTDWSSLQPRRSQAFVKLEKGIDPRRVNAELAEYLRRLTPAPVLFAGA
                     SHLRLYLQSLREMHYTPDFHSADTGDDFPKAYLPLLYALMWVALFVLGLAVINFINLS
                     SAQSFQRVKEVGVRKVLGSSQGALIGQFLIETLMLTTCALALSLLLLWPVLWLFGDSI
                     PAGVRFSFLDGGNWLFLLGITLFTTLVAGYYPARLMSSYLPVFTLKGSLDKKGKGGVG
                     LRRALIIFQFSISLLFIIGSLVIGRQVRYMKNSDKGFNSEGVITVDHPGAGKGQMRQY
                     AEAVSNLPGVQQAILQGHAPMGRIHPTATFVYRWDNMQKMREMTVEAEIGDSNFIPFY
                     RMRLVGGSDVYEGENIVPVLINETYARILGFNDPKQACGAQLLYRENGLWYSISGVVK
                     DYHQSSFHETIRPLIIITWLSDAKSVAIRLGVRDSAKKVIAALASKWKEFFPGVPFTP
                     TSLQDTVDQLYLEENRAALLMQSVTVIMILVSCMGLFGLALFSASRRAKEIGIRKVMG
                     ATVAGLSLLLSREFLSLVVAATVIASPLAWFLVNSWLRDYGYRTALDGWVWVQAGAVV
                     LVLAVVTVGIQAYRVASMNPVEVLRED"
     gene            2983..>4111
                     /locus_tag="VKQ52_03230"
     CDS             2983..>4111
                     /locus_tag="VKQ52_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HLZ86222.1"
                     /translation="MSRILLFSGGRSFDNASMTLARALIEKGHHVLYFDTTEERAYLE
                     QQGFEVHSIKAGDLAENETGPKTAPAASRRHSVIRQKMRYLDRLYSFGERWIEQNSID
                     LLLLDANALPFAVPFLKKKIPIINLTCTLSSVHRHNGPPVFSHLLPAKKYSLLRRIAY
                     QTAWLRIFFPQYLRDGVEALKLRMAFGTQPAEDVPARIKKYGGKVRRTEYGLRLLAPE
                     IVMGPGCIDFPDARTPGKLYANACICEDRKEESFDAGCLDKQKPLIYCAIGTCSNAYL
                     YKERFQQTVINAMGMLPDYQLVIQGIEKEKFNYPGPIPGNVRIFKTVPQLELLQRARI
                     FITHGGFSSIRESLFYGVPMIVFPGWHDQSGNAARVVYHGLG"
BASE COUNT          963 a         1042 c         1129 g          977 t
ORIGIN      
        1 ctttgggcaa gcagaaagac aaagaaaatc aggcacttag atcggcgact aatcccaagt
       61 ctgcgagaaa atggttcgag aaatttccag tccggtgcac tttgactttc agcaacttat
      121 aagagcttac aaaagtgagc tctttttttt gggaaaaatg tcccaacaat gtcccgacaa
      181 acggcagata aagacggatt caatttacga aatccctttc aatttgaaca ccgtttcacc
      241 gggacattaa cacccgtttc tgaggctgcc aacggacatg cccacgtgca accggacagc
      301 ggctgtatcg ctaacgtacg ggcaggccga acgagggaat agtatttccc tgacaatcac
      361 ggtatttata ggtctggcat agaattatcg ggcatgcctc cattaaagcc ttatcttaat
      421 gtctgtgaat gtcatctcgc tgaaaatagc ctggcgcaat acccttcgcc aacggtggta
      481 ttcgctgatc catgtccttg ggcttgccat aggcatctgc gtttgtatta tcatctttct
      541 tatcgggcgc tacgagctga gcttcgataa ttttcaccct gatgcataca ggatctatcg
      601 gatagtaggc gaagtggagg ataaggacgg caacctgatc tttctcaaca gcccattaag
      661 ggaagaggcc ggcatcgaac atctgatcgc cgggttcgaa gccaagacag ctttccatat
      721 atttggctcg accatctcag taccttcgcc aaacggccag gtgacgaagg tctacgacaa
      781 caggcaggat gacagctacg ccctgtccac cattttgacg gaccccgatt tcttccgcct
      841 ctttccacat gagtggctgg ccggcagccc ccaggtgttg gatcagccgg gacgcatagt
      901 gctgaccgct agtgtagccg gtaaatattt tgggccggga agggtggagc agatgcttgg
      961 ccggaaggtg atctatgacg acagccttac gctaacggtg gcgggcatcg tacgcgattg
     1021 gaaccggcct tccgatctca attacacttc ttttatctcg atcaagaccg cgccggcaac
     1081 ctggcttcgc gaacgtatcc ccacgacaga ctggagcagt ctgcaacccc ggcgctccca
     1141 ggcgttcgta aaattggaaa aaggcataga cccccgccgg gtgaatgcag agctggctga
     1201 atacctgcgc aggctgacgc ctgccccggt cctttttgcg ggcgccagtc atctgcggct
     1261 atacctgcag tcgctgcgcg agatgcatta tacccccgat tttcattccg ccgacacggg
     1321 tgacgatttc ccaaaagctt atctgccgct gttgtatgcg ttgatgtggg tggcgctatt
     1381 cgttcttggg ttggccgtca ttaatttcat caacctctcc agtgcccaaa gtttccagcg
     1441 agtgaaggag gtaggtgttc gcaaagtgct gggcagcagt cagggagcgc ttataggaca
     1501 attcctcatc gagacattga tgctgaccac ctgtgcgctg gcgctttccc tgttgctgtt
     1561 atggccggtg ctgtggctgt ttggagacag tattccggcc ggggtacgat ttagtttcct
     1621 ggatgggggc aattggttgt tcctcctcgg gataacccta ttcacgactt tggtggcagg
     1681 gtattatccg gcccggctga tgtcttctta tctgcccgtg ttcaccctca agggatcgtt
     1741 ggataaaaaa ggaaagggtg gcgtgggact gcggcgggca ttgatcattt tccaattttc
     1801 catttcattg ctcttcatca tcggctcgct tgtcatcggg cggcaggtga ggtatatgaa
     1861 aaattcggat aaaggtttta acagtgaggg ggtgataacg gtggatcatc ctggagccgg
     1921 aaagggtcag atgcggcaat acgccgaggc ggtgagcaat ctgccggggg tacaacaggc
     1981 cattttgcag gggcatgcgc ccatgggcag gatccatccg acggcgacat ttgtctaccg
     2041 ttgggataac atgcagaaaa tgcgggagat gacggtggaa gcggagattg gggacagtaa
     2101 ctttattccc ttttaccgga tgcggctggt gggggggtct gatgtctatg aaggggagaa
     2161 catcgtcccg gtgctgatca atgaaacata tgcccggata ctgggattca acgatccgaa
     2221 gcaggcctgc ggagcgcagc tgctttaccg ggagaatggc ctgtggtata gtatctcagg
     2281 ggtggtgaag gattatcacc agagctcttt ccatgaaacg atccggccgt tgatcatcat
     2341 tacctggctg tccgacgcta aaagcgtggc gatcaggctg ggggttaggg attcggcgaa
     2401 gaaagtgata gctgccctgg catcgaagtg gaaggagttt tttccgggtg tcccatttac
     2461 ccccacatcc ctgcaggata cggtcgacca gctgtacctc gaggaaaacc gggcggccct
     2521 gctgatgcaa tcggtaacgg tgatcatgat ccttgtctca tgcatgggct tgtttggact
     2581 cgcattattc tccgcaagcc gccgggcaaa ggagatcggt atccgcaagg tcatgggggc
     2641 taccgtcgcg gggctgtcat tgctgctaag ccgcgagttc ctgtcactgg tggttgccgc
     2701 gaccgtgatc gcctcgccgt tggcatggtt cctggtcaat agctggctgc gggattatgg
     2761 ctatcgcacg gcgctggatg gctgggtgtg ggtgcaagcc ggcgctgtgg tgctggtact
     2821 ggccgtggtg acggtgggga tccaggcata ccgggtggcc agcatgaatc ctgtggaggt
     2881 attgcgggag gactaaccgg cccgtttttc gtaaaacccg cagaggcatt gggtagtatt
     2941 acaaccgggg aatgctccac taatccttat tttagaacaa caatgagtag aatcctgttg
     3001 ttctccggcg gccggtcttt tgacaatgca agcatgacct tagcaagggc attgatcgaa
     3061 aaaggtcatc atgtactcta tttcgacact acagaagaac gggcttatct ggaacagcaa
     3121 ggatttgagg ttcattccat aaaagccggc gacctggccg aaaacgaaac ggggccaaag
     3181 acagctccgg ccgcctcccg tcgccattcg gtcatccggc agaaaatgcg ctacctggat
     3241 cgcctctatt cattcggcga acgatggata gaacagaaca gcatcgacct gctgctcctc
     3301 gacgccaatg cgcttccctt tgcagtaccc tttttaaaga aaaagatacc cattataaat
     3361 ctcacctgca ccctgtcctc agtgcaccgg cataacggcc cacctgtctt ttcacatctc
     3421 ctacccgcaa aaaaatacag ccttctccgg aggatcgcct atcaaacggc atggctgagg
     3481 atctttttcc cgcaatatct cagagatggt gtcgaagccc ttaagcttcg catggcattc
     3541 ggaacacaac ccgctgaaga tgtcccagcc cggatcaaaa aatatggcgg gaaggtcagg
     3601 agaaccgaat atggccttcg gctcctggct ccggaaatag taatggggcc aggatgcatt
     3661 gatttcccag acgcccgtac gccggggaaa ttatatgcca atgcctgtat atgcgaagac
     3721 cgaaaagagg aaagcttcga tgctggctgc ctggacaaac aaaagcccct gatctactgc
     3781 gccattggca catgcagtaa tgcttacctg tataaagaga ggtttcaaca gaccgtaata
     3841 aacgccatgg gaatgttgcc agactatcag ttggtcattc aaggtataga aaaagagaaa
     3901 ttcaactatc ccggaccaat tcccgggaat gtcaggatat tcaagacggt tcctcaactg
     3961 gaactacttc aacgtgccag gatctttata acacacggcg ggttctcatc gattcgggaa
     4021 tccctcttct atggtgtgcc catgatcgta tttccgggat ggcacgatca atccggcaac
     4081 gcagcccgcg tggtctatca tggccttgga t
//
LOCUS       DATGFG010000172         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_544343, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000172 DATGFG010000000
VERSION     DATGFG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544343"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2125
                     /locus_tag="VKQ52_03235"
     CDS             <1..2125
                     /locus_tag="VKQ52_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ86223.1"
                     /translation="ALFIEVGAGRGLLGLLKEHANNERRPNGVNIIRHQNEKENDAKY
                     FTERLGQLWAHGVEIDWQSFAAGGMYHRVSYPTYSFEPSIFPAEVNPPAEKAGSGANS
                     AAGQGSLKDWIYYPVWKSSFLPSPAATTGRKCFLYFSTGDPFSDSLRKQLIKDDDVLI
                     EVFMGDQFTIKNRHAFVLDPMRSAHYNALAGELKNSGVPVTDIVYSWCIGADATRMEL
                     KADNREIHLAYFSLAEIVRMLFRNTIPVKNGITILTDSLHKVSGYEEGSCVQSLVLGL
                     LNVIPQEYPIPCRSIDIVIAGHPGETAGALSGEIRNENFRDRIVAIRNGRRWIQDYQK
                     DPSAFVMPEENKKIKNGGVYLITGGLGNVGFLLAKHLVSRYNAKVVLTGRRDISGYPE
                     SNEGTRRLKALEGLTRDVRYRKVDIGNREELTALSRSIENDLGRINGIIHTAGNTDRD
                     CFELIEDLTPQHSIAVFLPKVLGVRNLYEVFGDYNADFVWITSSIASIVGGLKFSSYS
                     SANAFMESFVSMKGKELPNWKCIGLAEILPTEIETREKEAAKRQFLTPDELISLFELS
                     LHKDLPVIFETIQDLPSRFEEIFAGDKGTDNPVAGEKIGKAERPKLSNSFVPPNSNTE
                     KTVVQLMENFFGIAGLGVTDNFFDLGGDSLMAMSFLTKVKNVFTVNITIKFFFDNPTA
                     NVISSKINEIKSLTEVKKHTSKKII"
     gene            2129..>2703
                     /locus_tag="VKQ52_03240"
     CDS             2129..>2703
                     /locus_tag="VKQ52_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLZ86224.1"
                     /translation="MSIVELLHRLKDSNIELGLTGDDIEVNFDGQELPAGIQEEIRDN
                     KTAIVAFLKEMSAGHTLSAFVRERPAQIPLSYAQERLWFIDELSGSIQYHMTISFRLK
                     GPLDREGLAYSFQAVIRRHESLRTVIREHHGEAYQQVLPAGGWSMDYREDPEVDEVSL
                     RLHIEAVFARPFDLERDHMLRAELIRVGEED"
BASE COUNT          698 a          620 c          759 g          626 t
ORIGIN      
        1 ggcgttattc atagaggtag gcgccggccg ggggttgctt ggtttgttga aagagcacgc
       61 caataatgaa agaaggccga acggagtaaa tatcatccgg caccaaaacg agaaagagaa
      121 cgacgcaaaa tacttcacgg agcggctcgg tcaattgtgg gcgcatgggg tcgaaataga
      181 ctggcagtcg tttgctgccg gcggcatgta ccatcgggtc tcctacccca cctattcctt
      241 tgagccatcc atctttccgg ccgaggtgaa tccccccgca gaaaaggcgg gctccggggc
      301 taactccgcg gcaggtcagg ggagtctaaa agactggatc tattacccgg tttggaagag
      361 ttccttttta ccttccccgg cagccacgac aggcagaaaa tgttttttat atttttcaac
      421 cggggatccc ttttcggatt cattgaggaa gcaattgata aaggacgatg atgtcctgat
      481 cgaagtattc atgggagacc agttcacgat aaagaacagg catgcttttg ttctggaccc
      541 aatgagatca gcccactata atgccctggc gggggaattg aaaaattccg gggtgccggt
      601 taccgatatc gtctattcct ggtgtatcgg tgcagatgcg acgagaatgg aattaaaggc
      661 tgataaccgg gagatccacc tcgcttattt tagtcttgcc gagatcgtcc gaatgctatt
      721 caggaatact attcctgtaa agaacgggat tacgatcctg accgattccc tgcataaagt
      781 gtccggctac gaggaaggat cctgtgtcca atccctcgtg ttgggattac tgaatgtcat
      841 cccgcaggaa tatccgatcc cctgccggag tatcgatatc gttatcgccg ggcatcccgg
      901 ggagaccgcc ggagcactgt ccggcgagat caggaatgaa aatttcaggg accggatcgt
      961 cgcgattcga aacggccgga gatggataca ggactaccag aaagaccctt ccgcatttgt
     1021 tatgccggag gaaaataaaa agataaagaa cggtggggtc tatttgataa cgggagggct
     1081 gggcaatgtg gggtttttgc tggccaaaca cctggtaagc cggtataatg caaaagttgt
     1141 actgacaggg agaagggata tctccggcta ccccgaatcc aatgaaggga caaggcggtt
     1201 aaaagccctg gaagggttga ccagggatgt acgatacagg aaagtggata tcggcaaccg
     1261 ggaagaactg acggcattat ccaggagcat agaaaatgat cttggcagga tcaacgggat
     1321 cattcatact gcaggcaata ccgatcggga ttgttttgaa ttgatcgaag atctgacgcc
     1381 acagcactcc atcgcggtgt ttttgccaaa ggttcttggg gtcaggaatt tatacgaggt
     1441 gttcggggat tataatgccg actttgtctg gatcacctcc agcatcgctt ccatcgtagg
     1501 cgggttgaaa ttcagttcgt attcttctgc caatgccttt atggagtcat ttgtttccat
     1561 gaagggcaaa gagctgccga actggaaatg tatcggttta gccgaaatac tgccgacgga
     1621 gatcgaaacc agggagaagg aagccgcgaa acggcaattc ctgacccccg atgaacttat
     1681 ttccttattc gaattgagct tacataagga tctgcccgtc attttcgaga cgatccagga
     1741 cctgccgtcc cgcttcgagg agatatttgc cggggataaa ggcacagaca atcctgttgc
     1801 gggggaaaag atcgggaagg cggagcggcc aaaattgagc aattcatttg tgccgccgaa
     1861 ttcgaatacc gaaaagacgg tggttcaact gatggagaat ttcttcggca ttgccggatt
     1921 gggggtcacc gacaattttt tcgatctggg tggcgattcg ttgatggcca tgtctttttt
     1981 gacaaaggtg aaaaatgtct ttaccgtcaa tattaccata aaattctttt ttgataatcc
     2041 cacagccaac gtgatatcgt caaagatcaa tgaaataaaa tcgctgacgg aggtaaagaa
     2101 acatacttca aagaaaatca tttagcatat gagtatcgtc gaattgctgc accggttaaa
     2161 agacagcaac attgaactgg gtttgacggg tgatgatatc gaagtgaatt ttgacggcca
     2221 ggaattgccg gccggcatcc aggaagaaat acgcgacaac aagaccgcga tcgttgcctt
     2281 tctgaaggag atgtccgcgg gacatacctt atccgccttt gtgcgggagc ggccggcgca
     2341 gatacccctg tcctatgcac aggagcggct ctggtttatc gacgagctct cggggagcat
     2401 acagtatcat atgaccatct ccttccggct gaaaggcccg ctggacaggg aggggctggc
     2461 gtatagcttc caggccgtca tccgccggca tgagtccttg cggacggtga tccgtgaaca
     2521 tcacggggag gcctaccagc aggtgttgcc tgccggcgga tggagcatgg actatcgcga
     2581 ggacccggaa gtggatgaag tatcccttcg cctgcatatc gaagcggtct ttgcccgacc
     2641 gttcgacctc gagcgcgatc atatgcttcg cgcggagctg atccgggtag gggaagagga
     2701 cca
//
LOCUS       DATGFG010000173         6716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1108405, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000173 DATGFG010000000
VERSION     DATGFG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108405"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..721
                     /locus_tag="VKQ52_03245"
     CDS             137..721
                     /locus_tag="VKQ52_03245"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HLZ86225.1"
                     /translation="MEFAELDLSREYSYADYLQWTFEERLEIIKGKLFKMSPAPSRIH
                     QKLSGEIFGVLFNFLKGKTCEVYSAPFDVRLPRKSLDDKEIITVVQPDLCVICDPAKL
                     DAKGCLGAPDIIIEILSPGNNKKELENKYEVYEEAGVKEYWLILPQEQACFQYILNDD
                     GRYMGKRPLTEGNILTTPLLPGFELDLEEVFGVL"
     gene            799..4437
                     /gene="dnaE"
                     /locus_tag="VKQ52_03250"
     CDS             799..4437
                     /gene="dnaE"
                     /locus_tag="VKQ52_03250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HLZ86226.1"
                     /translation="MYKFSHLHTHTQFSLLDGAASIQSLYKKAIKDGMPALAITDHGN
                     MFGAFEFVAEAYKHKNEDGSLKVKPIVGCEFYVVEDRFRKTFTKEQRDERYHQILLAK
                     NKQGYQNLIKLTSLGYTEGLYSKYPRIDKKLILQYHEGLIATTCCIGAYVPQTILHES
                     EEKAEQEFKWWLDLFGEDYFIELQRHNILEQQKINQVLLGFAKKFGVPVIATNDSHYT
                     DKDDANAHDILLCINTGEKQATPGYDDIVNDDLNTKNRRFKFPNDQFYFKTTEEMKTL
                     FSDIPESIDNTNMIVDRVEVLNLKKDILLPAFPIPKEFQIHNDSNLDQWEYLKYLTQE
                     GAKLRYSDLNAEIQERLDFELFTIKTMGFAGYFLIVSDFIKAGRDMGVFIGPGRGSAA
                     GSVVAYCIGITNIDPIKYKLLFERFLNPDRKSMPDIDTDFDDEGRGKVIEYVVGKYGK
                     NQVAQIVTYGTMAAKMSIKDVARALDLPLPESNALAKLVPERPGIELKRVLHAPMTTK
                     DGEKSLEEKDGVGPDDLQNVKLLRDIYNGDDIRSKVLHEAERLEGSVRNTGLHAAGII
                     IAPDDLMTILPVCTARDSDLLVTQIEGSVIEEAGVIKMDFLGLRTLNILKTALELIKE
                     NHGVDIVIDDIPLDDVKTFELYQRAETIGTFQFESPGMQKYLKDLKPDKFNDLIAMNA
                     LYRPGPIAYIPNYIDRKHGREPIIFDLPEMEEYLGDTYGITVYQEQVMLLAQKLAGFS
                     KGDADVLRKAMGKKQKSILDKMKSQFVKGASAKGFAAETLEKIWTDWEAFAQYAFNKS
                     HSTCYAFVAYQTAYLKAHYPSEYMAAVLNHAGSIEKITFFMEECKRMGLKVLGPDINE
                     SKKGFAVNQNGEIRVGLGGLKGVGEAAVEGIISERSKDGNFTSIFDMIKRINQRTVNK
                     KTLENLAYAGSFDCFREMHRAQYFFMAPGDTSTGLEKIIKFGNVYQTNSHQTSNSLFG
                     DLAMPEIAVPKIPNCEPWSLTELLEHEKDVTGMFMSGHPLDHFKFEITHYGITQLGEF
                     NEIKEAVGLQSNPGKAYRLAGLVVDAQHRVSKTGKQFGSFVIEDYTGKAEFMLWSEDY
                     ARYSNYLEKGKNLFMTGSFKARFNTAQFEFKIDRMMLLESIKPILTKQVIMDVEARFV
                     NEELVQFLEKNVKKYPGKSGLKFNIVESKTHARASLFTMGNGFEMNDEMSAFLEKTPE
                     VEVQVVTT"
     gene            4708..5025
                     /gene="trxA"
                     /locus_tag="VKQ52_03255"
     CDS             4708..5025
                     /gene="trxA"
                     /locus_tag="VKQ52_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HLZ86227.1"
                     /translation="MALELTDTNFQDKVIASDKLTVVDFWAEWCGPCRAIGPVIEELS
                     KEYDGKINIGKVNVDHNPQLSINYGITSIPAILFIKDGKVVDKLVGAQPKSNFVKKIE
                     SHL"
     gene            5238..>6716
                     /locus_tag="VKQ52_03260"
     CDS             5238..>6716
                     /locus_tag="VKQ52_03260"
                     /inference="COORDINATES: protein motif:HMM:NF037004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLZ86228.1"
                     /translation="MRMLLALCLYTFLLLSVNAQSPAPDSKATDAFLITRMVEKFHVQ
                     PRQLDKTMSAAIYTQLLRSLDDQRLFFTQSDIAQLSAYRYRLDEEILGRKTAFLQLLT
                     GLYQQRLIESDTMIDRITQKPFTFTTKEVLTVAEDSSYPADLAARRAKLYKLVKLSIG
                     SRLAAAIVKQEEAGKGGAMQGSGGVGSGAGQSAGGAGDMLRGKTRQQLIDSLEPKLRK
                     RAVVSIKRMIKRLLQSPTGIGNMIGIIYCQALAECYDPHTAYFSPDEKAQFETAIGNK
                     PLSYGLTLTEDEDGHAQVGHLLPGGPAFQSGALNEGDRIIAVRWDGKEAIDVTDATAA
                     ELYRILSTEGGDRLTIGIRKADGTTREVSLQKARVAGVADDDEDKVKGFLLKGARTVG
                     YISLPAFYEDWEDSRGVNGCANDVAKEIIKLRKENIGGLILDLRYNGGGSMDEAVQLS
                     GLFIDAGPVGQVVSRDAKVYTLKDVNRGTVWAGPLLVLVNGSS"
BASE COUNT         1615 a         1425 c         2199 g         1477 t
ORIGIN      
        1 gtgaggggtt ggtttggggg tgaggttgat ggggtgggca gggcatggat ggtgagtttg
       61 tgaggcgggg cgaggggggt gaagttcggg tttcctaaag aatttagata tatttgtacg
      121 tataaagagc gttaatatgg aattcgctga actcgatctt tcccgggaat atagctatgc
      181 cgattatttg cagtggacgt ttgaggaacg gctggagata atcaagggta agttgtttaa
      241 aatgagcccc gcaccgagcc ggattcatca aaaattgtcg ggggaaattt ttggggtgct
      301 gttcaatttc ctaaagggca aaacgtgtga ggtgtattcc gccccttttg acgtgcggct
      361 accccgcaag tccctggatg ataaagagat tataacggtg gtacaaccgg atctatgcgt
      421 catttgcgac cctgcgaaat tggatgcaaa aggatgtttg ggcgcgccgg atattattat
      481 cgaaattctc tcgcccggga ataataagaa ggaactggag aataaatatg aagtttatga
      541 agaagccggg gtaaaagaat attggttgat cctaccccag gagcaagcct gctttcaata
      601 tatcctcaac gacgacgggc gctatatggg caaacgtccg ctgaccgaag ggaacatcct
      661 caccaccccc ctcctccccg gattcgaact cgacctggaa gaggttttcg gcgtcctgtg
      721 accttgtgga tataatgtga cttacacccc tggcagtgaa cgatatttgt cgggttttgt
      781 tagttaattg caggtcctat gtacaagttt tcccatctac atacgcatac gcaattttcc
      841 ctgctggacg gggcggcgtc gatccagagc ctttataaga aggcgatcaa ggacggcatg
      901 ccggcgctgg ccatcaccga ccatggcaat atgtttggcg ccttcgagtt tgtggcggaa
      961 gcctataagc ataagaacga ggatggcagc ctgaaggtga agccgatcgt ggggtgtgag
     1021 ttctatgtgg tggaggaccg gttccggaag acctttacca aagaacagcg ggacgaacgt
     1081 tatcaccaga tcctccttgc taagaataag caggggtacc agaatctcat caagctcact
     1141 tcccttggct ataccgaggg gctttacagc aagtatccgc gtatcgataa gaagctgatc
     1201 ctccaatacc acgaggggct tatcgccacc acctgctgta tcggggccta tgtgccgcag
     1261 accattttac acgagagcga ggaaaaagcc gagcaggaat tcaaatggtg gctggacctc
     1321 ttcggagagg attattttat cgagctgcag cgccacaaca tcctggagca gcagaagatc
     1381 aaccaggtgt tgcttggttt tgcgaagaag ttcggggtgc cggtgatcgc gacgaacgat
     1441 agccattaca ccgataagga cgatgccaac gcgcatgata tcctgctctg tatcaatacc
     1501 ggtgaaaagc aggctacccc ggggtatgac gatatcgtga acgatgatct gaacaccaag
     1561 aaccgccggt tcaaattccc gaacgaccag ttctatttca agacaacgga ggaaatgaag
     1621 acgttgttct ccgatatccc ggaatccatc gacaatacca atatgatcgt ggaccgggta
     1681 gaagtgctga acctgaagaa ggatatcctg cttccggcct tcccgatccc gaaagagttc
     1741 cagatccata acgacagtaa cctggaccag tgggagtacc tcaaatacct gacccaggag
     1801 ggggcgaagc tccgctacag cgacctgaac gcggagatcc aggagcggct ggatttcgag
     1861 ctttttacga tcaagacgat gggctttgcc gggtacttcc tgatcgtttc cgatttcatc
     1921 aaggcgggaa gggatatggg cgtctttatc ggtcccggtc gtggctccgc ggcgggtagt
     1981 gtcgtcgcgt attgtatcgg catcacgaat atcgatccca tcaagtataa gctgctgttc
     2041 gaacggttcc tgaatcccga ccggaagtcg atgccggata tcgatacgga cttcgacgat
     2101 gaaggccgtg gtaaagtaat cgagtacgtg gtggggaaat atggcaagaa ccaggtggcg
     2161 cagatcgtga cctatggtac gatggcggcg aagatgagta tcaaggacgt ggcgcgggcg
     2221 ctggacctgc ccctgccgga gtcgaatgcg ctggcgaagc tggtgccgga gcggccgggg
     2281 atcgagttga agcgggtgct gcatgccccg atgacgacga aggatggcga gaagtcgctg
     2341 gaagagaagg atggggtcgg acccgatgac ctgcagaatg taaagttgct gcgcgatatc
     2401 tataacggcg atgatatccg aagcaaggtg ttgcatgagg cggagcggtt ggaggggtcg
     2461 gtgcggaaca cggggttaca tgcggcgggg atcattatcg cgccggatga cctgatgacc
     2521 attttgcccg tctgcacggc aagggattcg gacctgttgg tgacgcagat agaaggaagc
     2581 gttatcgagg aagcaggcgt tatcaagatg gactttctcg ggctgcggac gctgaatatc
     2641 ctgaagacgg cgttggaact gataaaagag aatcacgggg tggatatcgt tatcgacgat
     2701 attccgctgg acgatgtaaa gacctttgaa ttgtatcagc gagctgaaac gatcggtacg
     2761 ttccagtttg aaagtccggg aatgcagaaa tacctcaagg acctgaagcc ggataagttt
     2821 aacgacctga tcgcgatgaa cgcgttgtac cggccggggc cgatcgccta tatcccgaat
     2881 tatatcgacc ggaagcatgg gcgggagccg attatcttcg atctgccgga gatggaggaa
     2941 tacctgggcg atacctatgg catcaccgtg taccaggaac aggtgatgtt gctggcgcag
     3001 aagctggccg ggttcagcaa gggggatgcg gacgtgttgc ggaaggcgat ggggaagaaa
     3061 caaaagtcga ttctcgataa gatgaagagc cagttcgtga agggggcttc ggcgaagggt
     3121 tttgcggcgg agacgctgga gaagatctgg acggactggg aggcatttgc acagtatgcg
     3181 ttcaacaagt cgcactccac gtgttatgct tttgtcgctt accagacggc ctacctgaag
     3241 gcgcattatc cctccgagta tatggcggcc gtgctgaacc atgcgggcag catcgagaag
     3301 atcacctttt ttatggagga atgcaagcgg atgggcctga aggtattggg gccggatatc
     3361 aacgagtcga agaagggttt cgcggtgaac cagaatgggg agatccgggt ggggcttggc
     3421 gggctgaaag gagtcggcga agcggcagtg gaagggatca tcagcgaacg gagcaaggac
     3481 gggaatttca cttcgatatt cgatatgatc aagcggatca accagcggac ggtgaacaag
     3541 aaaacgctgg agaatcttgc ctatgcgggg agcttcgatt gtttcaggga aatgcaccgg
     3601 gcgcagtact tcttcatggc gccgggggat acctcgacgg ggctggagaa gatcatcaaa
     3661 ttcgggaacg tgtaccagac caattcccac cagacaagca atagtctttt tggcgatctg
     3721 gcgatgccgg agatcgcggt gccgaagatc cccaactgcg agccctggtc gctgacggag
     3781 ttgctggagc atgagaagga tgtgacgggg atgtttatga gcggacaccc gctggatcac
     3841 ttcaaattcg agataacgca ttatgggatc acgcagcttg gggagttcaa tgagatcaag
     3901 gaggcggtgg gcctgcaaag caatcccggg aaggcgtacc ggctggcggg gctggtggtg
     3961 gatgcacagc accgggtgag caagaccggg aagcagttcg ggtcgtttgt gatcgaggat
     4021 tataccggga aggcagagtt catgctgtgg agtgaggact atgcacgata cagcaattac
     4081 ctcgagaagg ggaagaacct gttcatgacg ggtagcttca aggcccggtt caatacggcg
     4141 cagtttgagt tcaagatcga ccggatgatg ctgttagaga gcattaagcc gatcctgacg
     4201 aagcaggtaa tcatggatgt ggaagcgagg tttgtgaatg aggagctggt gcagttcctg
     4261 gaaaagaatg tcaagaagta tccggggaaa tcggggttga aattcaatat agtagagtcg
     4321 aagacgcatg cccgggcgag cctttttacg atggggaacg gcttcgagat gaatgacgaa
     4381 atgtctgctt tcctcgagaa gacgccggag gtagaggtgc aagttgtcac gacatgatga
     4441 ttgtccttcg gacgaggcgg cagttccgcc gcctttggaa ggatgaaagc ggcggttccg
     4501 ccgcctttgg agcggccagg ggtgacagga ttgccggtta gggatgggaa ccgggtgatt
     4561 tttggtcctg gtgcgggatt tgacttagag ggggataaag gatgtaagca tgaatcgggc
     4621 gattgtcggg aaaatgacgt aaaagttgag caaataagtg gacatttgtg aagtaaaata
     4681 aacacataac ataaaaaaca cgttattatg gctttagaat taactgacac caacttccag
     4741 gacaaggtga tcgcttccga taagctgacc gtggtagatt tctgggcaga gtggtgtggc
     4801 ccctgtcgcg ctatcggacc cgttattgaa gagttgtcca aggaatatga tgggaagatc
     4861 aatatcggga aggtgaacgt agaccataac ccgcaattat cgatcaatta tggtatcacg
     4921 agtattcctg ccatcctttt tatcaaggac ggtaaggttg tggacaagtt ggtcggtgca
     4981 cagccgaaat cgaattttgt gaagaagatc gagtcgcatc tgtaagatat tgttggataa
     5041 acaatcgaac cccgcgcttt tgtgcggggt ttttattttg tgggtggtag tttgtggcgg
     5101 ggcagtttgt gcggggcagt ttgtgcgggg tagtttgctg ggtgttagag gaccgggaga
     5161 agaggccggc ggagggagag gtggttgccg tgttgcgcta ttcctgagat gaacattttt
     5221 tcctattttg gggaattatg cgaatgctgc tggccctttg cctgtatacg tttctgctgt
     5281 tgtccgtaaa tgctcaatcc cctgccccgg atagtaaggc gaccgatgcg tttctcatca
     5341 cccggatggt ggagaagttc catgtgcagc cgaggcaact ggacaagacg atgtcggcgg
     5401 cgatctatac ccaactattg cgaagcctgg atgaccagcg attgttcttt acccaatccg
     5461 atattgcgca gttgtcggcg taccggtatc gcctcgatga ggagatcctt gggcgcaaga
     5521 cggctttttt gcagttgctg accgggcttt accagcagcg gctgatagaa tcggatacga
     5581 tgatcgaccg gatcacgcag aagcccttta cgtttacgac gaaggaggtg ttgacggtgg
     5641 cggaggatag ctcctaccct gccgatctgg ctgcgcggcg ggcgaagttg tataagctgg
     5701 tgaagttgtc gatcgggtcg cggctggcgg cggcgatcgt taaacaggag gaggccggga
     5761 agggaggagc aatgcagggg tcgggaggcg tgggttccgg tgcggggcag agtgcggggg
     5821 gcgcagggga tatgctgcgg ggtaagacga ggcagcagtt gatcgacagc ctggagccga
     5881 agctgcggaa gcgggcggtg gtgtcgatca agcgaatgat caaacgcttg ttgcagagcc
     5941 ctacagggat cgggaatatg atcgggatca tctattgcca ggcgcttgcg gagtgttatg
     6001 atccgcatac cgcctatttt tccccagacg agaaggcgca gttcgagacg gcgatcggca
     6061 ataagccgtt gtcctatggg ctgacgctta ccgaggacga ggatgggcat gcgcaggtgg
     6121 gtcatttgct tccgggcggt ccggcgtttc agagcggggc gcttaacgag ggagacagga
     6181 tcattgcggt gcgctgggat gggaaggagg cgatagatgt gactgacgct acggccgcgg
     6241 agctttaccg gatattgagt acggagggcg gggacaggct gacgatcggc atccggaagg
     6301 ccgacgggac gacgcgggag gtgagtttgc agaaggcgag ggtcgcgggc gtggcggatg
     6361 atgacgagga caaggtgaaa ggttttttgc tgaagggcgc caggacggtg gggtatatct
     6421 cgctgccggc cttttacgag gactgggagg atagccgggg ggtgaatggg tgcgcgaacg
     6481 atgtcgcaaa agagatcatt aaactcagga aggagaatat cggcgggctg atcctcgatc
     6541 tgcgatataa tggcggcggg tcgatggatg aggcggtgca actgtcgggt ttgttcatcg
     6601 atgccggtcc ggtgggtcag gtggtctcgc gggacgcgaa ggtatataca ttaaaggatg
     6661 tcaatcgggg gacggtgtgg gccgggccgt tgctggtgct ggtgaacggg agcagt
//
LOCUS       DATGFG010000174         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1454064, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000174 DATGFG010000000
VERSION     DATGFG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454064"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VKQ52_03265"
     CDS             complement(<1..1327)
                     /locus_tag="VKQ52_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HLZ86229.1"
                     /translation="MVFDLDLIKKLYGVMPAKVDAARKLVGSPLTLAEKILYSHLFET
                     PTRAYHRGKDYVDFAPDRVAMQDATAQMALLQFMTCGRDKVAVPSTVHCDHLIQAKTG
                     AAADLATAKDVNKEVYDFLASISNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDS
                     HTPNAGGLGMVAIGVGGADAVDVMAGLPWELKMPKLIGVKLTGKMSGWTSAKDIILKV
                     AGILTVKGGTGAIVEYFGEGADSLSATGKGTICNMGAEIGATCSLFGYDGKMAAYLKA
                     TGREEVAALADGIRDYLRPDKEVYADPKKYYDQVIEIDLNTLEPYVNGPFTPDLAWPI
                     SEFAKAVKDNNWPEVLEVALIGSCTNSSYEDISRSASLAQQAIDKKLKTKAEFTITPG
                     SEQVRYTIEKDGFLKTFDSIGGVVLANACGPCIGQWARHIDDPNRKNSII"
     gene            1458..1679
                     /locus_tag="VKQ52_03270"
     CDS             1458..1679
                     /locus_tag="VKQ52_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86230.1"
                     /translation="MNRLKHFIEWQVFGVCTAIGERMGIATSRIRTWFIYISFLTMGS
                     PLIVYFILAFWMNIKRYILSARRNPLKYL"
     gene            1809..2801
                     /locus_tag="VKQ52_03275"
     CDS             1809..2801
                     /locus_tag="VKQ52_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ86231.1"
                     /translation="MFQFDFSYTDYQQLMNDLAQRLNVPVVNHQITFPEPIATGTLTF
                     VRLPNGIHVNIVNCRMNQDWLLCRRKMREQFYTLRFHELTIPGTLEIRIGDERLKESN
                     TVRAIAYLTNTLSDWAYLGAKGTVYKGIDVLFSPTWLGDYLGIQEIDDVLSAYLSLQV
                     ENVHREPLDSEYRRLMQEIVEVDEANPMRLAIIQNRVMLLIERFFLRIYERRKNAFLT
                     IPLSKADIDRVMLVEATLTKDIFEPAPTISQLARMVSISESKLKKDFKLLYGIPVYEY
                     FQKVRMQAAKDKLLAGGHSVKEVAMELGYSNLSNFTIAFKKEFGLLPSKLLTTY"
     gene            complement(2969..3793)
                     /gene="kdsA"
                     /locus_tag="VKQ52_03280"
     CDS             complement(2969..3793)
                     /gene="kdsA"
                     /locus_tag="VKQ52_03280"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HLZ86232.1"
                     /translation="MEFLHQLFRHQSYDKNNFFLIAGPCVVEGEELVMEVADTVSGIC
                     RNLGIPYVFKASYRKANRTSAGSFTGIGDDNGLSLIKKVGEKYKIPTTSDIHAHDEAA
                     PAAKYVDILQIPAFLCRQTDLLVAAAETGKVVNVKKGQFLSGPAMKFAVEKIKKAGNE
                     KIVLTERGTTFGYQDLVVDYRNIPWMQEHGAPVVMDCTHSLQQPNQTSGVTGGNPQLI
                     GTIAKAAIATGANGLFIETHPNPAIAKSDGANMLRLDLLQGLLEQLVRLRKVVSVL"
BASE COUNT          891 a         1124 c          908 g          938 t
ORIGIN      
        1 tgatgatcga gttcttccgg ttgggatcgt cgatatgccg cgcccattga ccgatacagg
       61 gaccacaggc gtttgcaaga actactcccc cgatgctgtc aaaagtcttc aggaaaccat
      121 ccttttcgat ggtataccgc acctgctcgg aaccgggggt gatggtaaac tcggcctttg
      181 ttttcagctt cttatcgatc gcctgctgcg ccagggatgc actgcggctg atatcctcgt
      241 aggaggaatt ggtgcagctg ccgatcaggg ctacttccag cacctcgggc cagttgttgt
      301 ccttgacagc cttggcgaat tcgctgatcg gccaggcaag atcgggggtg aacggcccat
      361 tcacataggg ttcgagcgtg ttcagatcga tctcgatcac ctggtcgtaa tattttttcg
      421 gatcggcgta tacttccttg tcgggacgca gatagtccct gatcccgtcg gcgagcgcag
      481 caacctcttc acggcccgta gccttcagat aggcggccat cttcccgtca tagccgaaca
      541 gcgaacaggt ggccccgatc tcggcgccca tattacagat ggtgcccttc ccggtagcgc
      601 taaggctgtc cgcgccctcg ccaaaatatt ccacgatcgc gccggtgcca ccctttaccg
      661 tcaggattcc ggcaactttc aaaataatgt ctttggcgga cgtccagcca ctcatctttc
      721 cggtcagttt caccccgatc agcttgggca tcttcagctc ccacggaagc cccgccatca
      781 cgtctaccgc gtcggcgccg ccaaccccga tggcgaccat ccccaaacca cccgcgttgg
      841 gcgtatggga gtcggtaccg atcatcatcc ccccgggaaa agcgtagttt tccaacacca
      901 cctggtgaat gatacccgca ccgggtttcc agaacccgat gccatattta tttgaaatag
      961 aggccaggaa atcatacacc tccttgttga catcctttgc cgtggccagg tcggcggcag
     1021 cgccggtctt ggcctggatg aggtggtcac agtgaacggt agaaggaacg gccaccttgt
     1081 cgcggccgca ggtcatgaac tgtaaaagcg ccatctgggc cgtcgcatcc tgcatggcca
     1141 cgcggtccgg tgcaaagtct acataatcct taccccggtg ataagcccgg gtcggggtct
     1201 caaagaggtg ggaatacaat atcttttcgg ctaatgtcaa cggactccca accagcttac
     1261 gggcggcgtc caccttggcg ggcatcactc cataaagctt cttaattaag tctaaatcaa
     1321 agaccatgat aaattttgtt tatatgttag acaagacgat tcccattccg gcgcgaccga
     1381 aagatttacc cgaatgcgac cgcaaattta acttaaaaat gcttcggccc ccaccgaatt
     1441 ttttataaat tagccttatg aacagactga aacactttat cgagtggcag gtgttcgggg
     1501 tgtgtacagc aattggtgag agaatgggta tagccacttc ccgtatccgg acctggttca
     1561 tctatatttc cttcctcacc atgggatccc ccctcattgt ctatttcatc ctcgctttct
     1621 ggatgaatat aaaaaggtat atcctctccg caagacgcaa tcccttgaag tatctctgat
     1681 tacccgaagt atctctgatt acccagttcc gcgactgcca gggcatgctt tttttcgtac
     1741 cttaccatgg taaggctccc cctatccgta cataaaaaag gtattataca tggactaaat
     1801 cctgatcctt gtttcaattt gatttttcct atacggacta tcagcagctg atgaatgacc
     1861 tggcgcagcg attgaatgtt cccgtagtca accatcagat caccttcccg gagcctatcg
     1921 ccaccggcac ccttaccttt gtccgcctgc cgaatggcat ccatgtcaat atcgtcaatt
     1981 gccgcatgaa ccaggactgg ctgctctgcc gccggaagat gcgcgaacag ttctacaccc
     2041 ttcgctttca cgaactgacc attcccggga cactcgagat ccgcatcggc gacgaacgcc
     2101 tgaaggagtc caacaccgtc cgcgccatcg cctatcttac caatacgctc tccgattggg
     2161 cctaccttgg agcaaaggga accgtctaca aaggcatcga cgtgctcttc agccccacct
     2221 ggctgggcga ctacctcggg atccaggaaa tagacgacgt cctttcggcc tatctctcgc
     2281 tccaggtgga gaacgtccat cgcgaacctc tcgatagcga atacagacgc ctcatgcagg
     2341 agatcgtgga agtggacgag gccaacccca tgcggctggc gatcatccag aacagggtca
     2401 tgctgctcat cgaacgcttc ttcctccgca tctacgagcg acgcaagaac gccttcctga
     2461 ccattcctct ctcaaaagct gatatcgatc gcgttatgct ggtggaagcc accctcacaa
     2521 aggacatctt cgagccggcg cccaccatca gccagctggc ccggatggtc tcgatcagtg
     2581 aatccaagct gaaaaaggac ttcaagctcc tgtacggcat tcccgtgtac gaatacttcc
     2641 agaaagtaag gatgcaggcg gcaaaggata aattgctggc cggaggccat tcagtgaagg
     2701 aagtggcgat ggaactgggc tattccaatc tcagtaactt taccatcgca ttcaaaaagg
     2761 aatttgggct cctgcccagt aagttgttaa cgacttatta aattcgcatt aaataatctc
     2821 tacagctatc caaaacacct ttccggatag gggaaactac ccatttatcg gtacctttgt
     2881 ttcagcaagg ttcttaatag ggtttattca ttgtcccgtt tttaattcct cacgtaatcc
     2941 aggaaagacc aatccggatc cacacccttt ataacacgga aactacttta cgtaatctta
     3001 ccaattgctc cagtaatccc tgtaaaagat ccagtcgtag catattggct ccatcgctct
     3061 tggctatggc agggttgggg tgcgtttcta tgaataagcc gttggcgccg gtggcaattg
     3121 cagctttcgc gatcgttccg atcaactgtg gattgccgcc ggtgactccg cttgtctggt
     3181 tgggctgttg cagcgaatgg gtgcagtcca tcactacagg agcgccatgc tcctgcatcc
     3241 agggaatatt gcggtaatcc acgaccaggt cctggtaacc aaaggtggtg cctctttccg
     3301 tcagtacgat cttctcattc cccgctttct ttattttctc gaccgcgaat ttcatcgcag
     3361 gaccactcag gaactgcccc ttctttacat tgaccacctt ccccgtctcc gcggcagcga
     3421 caaggagatc ggtctgccgg caaagaaagg ccgggatctg taaaatatcc acatactttg
     3481 cagcaggcgc cgcctcatcg tgcgcgtgaa tatccgacgt cgtcgggatc ttatatttct
     3541 cccccacctt ctttatcagg gaaagaccat tgtcatcgcc gatgcccgta aagctcccgg
     3601 cgctggtccg gttcgccttc cggtacgaag ccttaaaaac atacgggatg cccaggttac
     3661 ggcagatgcc ggacacggta tccgccacct ccatcaccag ttcctcgcct tccaccacac
     3721 agggccccgc gataagaaag aaattgttct tgtcgtagga ttgatgccga aataattgat
     3781 gaagaaactc catgccgcga aactacgttt tttttatttc ccgaaattca taggtttgca
     3841 acccaaaacc cccgacatgg c
//
LOCUS       DATGFG010000175         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_196399, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000175 DATGFG010000000
VERSION     DATGFG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196399"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VKQ52_03285"
     CDS             <1..936
                     /locus_tag="VKQ52_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86233.1"
                     /translation="VDYRDQRIDQSDFGIYQDYLNPLQEDSYTDKLSPDSARVRQFAA
                     YASVLLKNLGGFNLDLGGRYNNFSRYGNVFTYSVNPSFLINRRTKLFADLSSGFSAPT
                     LYQLYSPYRNPSGNLNPERTVSFEGGIQYSVSRLDLRALYFRRHTKDNIIFYTDANYN
                     SYYINLDKQDDHGFEVEGRYTYGPWTFTGNYTYTTGKVTTPVGGKDTTYNNLYQRPKS
                     LVNASIGLQPMKGLFLSIALRTAGRRISSIYGGPRAQADGFAYYTLNGYAEYRVCRLL
                     KLFADVKNITGQRYFDIPGYTSFGLNFMAGISVHL"
     gene            956..1489
                     /locus_tag="VKQ52_03290"
     CDS             956..1489
                     /locus_tag="VKQ52_03290"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HLZ86234.1"
                     /translation="MPEPSLQKRARLIFITGGARSGKSRYAQELALRQSDTPFYIATA
                     RIWDTDFRRRVDSHQQERDHRWALLEEEKWLSRLPLGGKVAVIDCVTLWITNFFSDLR
                     YDVDACLDACKQEIDALLQQDTVLLVISNEIGMGLHADTESGRKFIDLQGWINQSIAA
                     RADEVIFMVSGIPLKVK"
     gene            1498..2562
                     /gene="cobT"
                     /locus_tag="VKQ52_03295"
     CDS             1498..2562
                     /gene="cobT"
                     /locus_tag="VKQ52_03295"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HLZ86235.1"
                     /translation="MRTEIQTTTLRQQLQQLIDSKTKPLGALGRLEDIAIQIGLTQGT
                     AHPQLSRPYIIVFAGDHGIAATGLVNPYPQSVTAQMVLNFITGGAAINVFCRQHHLGL
                     TVVDAGINFDFEAGLFPGKLISEKIGYGTRNYLETEAITADEYTKALEAGKRVVTELY
                     ASGCNCVGFGEMGIGNTSSAALIMSYITELPVEECAGRGTGVNDEQLRTKRETLRKAY
                     ERHLPAIKARPGAQTILQCVGGFEVAMMTGAFLKAAELKMIILVDGFITTAALLLAKQ
                     LNPAVQDNCFFAHTSGENGHAAMLDYLGAKPLLNLGMRLGEGTGAAMAFPLLQSAVLF
                     LREMASFESAGVSKASPDEK"
     gene            2552..>3042
                     /locus_tag="VKQ52_03300"
     CDS             2552..>3042
                     /locus_tag="VKQ52_03300"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HLZ86236.1"
                     /translation="MKNEWKVFLAAIQFLTRIQVPAGIGHEPALLQQAPKYFPLVGWI
                     VGALSALFFLVFARYISTDVGILASMITGLLITGAFHEDGFADVCDGFGGGWTKEKIL
                     LIMKDSRIGAFGAMGLIFILGTKFLLLRELPGFVTTPGEASHLIFYPYRFFILVLISA
                     HSV"
BASE COUNT          665 a          922 c          836 g          619 t
ORIGIN      
        1 gtcgactatc gcgaccagcg catcgaccaa tccgacttcg gcatctacca ggactatctc
       61 aacccgctgc aggaagatag ctataccgat aagctaagcc ccgacagcgc cagggtcagg
      121 cagtttgccg cctacgcctc cgtactgttg aagaacctcg gcggtttcaa cctggatctc
      181 ggcggccgct acaacaactt ctcgcggtat gggaatgtct tcacctattc cgtgaaccct
      241 tccttcctga tcaaccgccg gacaaagctt tttgccgatc tctcctccgg cttctcggcg
      301 cctaccctct accagctgta ctctccctat cgcaacccct ccgggaacct caaccccgaa
      361 agaacggtga gcttcgaagg gggtatccag tacagcgttt cccggcttga cctgcgcgcc
      421 ctgtatttcc gccggcatac gaaagacaat atcatcttct ataccgacgc caactacaac
      481 agctactata tcaacctcga caaacaggac gatcacgggt tcgaggtgga aggaaggtat
      541 acctatgggc cctggacatt taccggcaac tatacctata ccaccgggaa ggtgacgaca
      601 cccgtgggcg gaaaagatac cacctacaac aatctctacc agcggccgaa gagcctggtg
      661 aatgcctcca tcgggttgca gcccatgaag gggctcttcc tgagcatcgc cctgcggacc
      721 gcgggccggc gcatctcctc gatctatggc ggaccgcgcg cgcaggcgga cggttttgcg
      781 tattatacgc tcaacgggta tgccgagtac cgggtatgcc ggctcctcaa gctgttcgcg
      841 gatgtaaaga acatcaccgg ccagcgctat ttcgacatcc ccggctatac cagcttcggt
      901 cttaatttca tggcgggaat cagcgtacat ttataggatc atgccagaac aaaccatgcc
      961 agaaccctcc ttgcagaaac gggcaagact catttttatc accggcggag cgcgtagcgg
     1021 caagagccgc tacgcgcagg agctggcgct gcggcaaagc gacactccct tctatatcgc
     1081 cactgcgcgc atctgggaca ccgacttccg gcgccgggta gacagccatc agcaggagcg
     1141 cgaccaccga tgggccctgc tggaagaaga aaaatggctt tcccgcctgc ccctcggcgg
     1201 aaaagtggcg gtcatcgact gcgtgacgct ttggatcacc aacttcttct ccgatctgcg
     1261 ctatgatgtc gacgcctgtc tcgacgcctg caaacaggag atcgacgcgt tgctgcaaca
     1321 ggataccgta ctgctggtca tcagcaacga gatcggcatg ggcctgcacg cggacaccga
     1381 atccgggcgg aaattcatcg atctgcaggg atggataaat cagtccattg ccgcaagggc
     1441 cgatgaggtt atctttatgg tcagcgggat accgctcaaa gtaaaataat acccagcatg
     1501 cgaacagaga tacaaacaac aaccttgcgg cagcagctgc aacagctcat cgacagcaag
     1561 acaaaaccgc tgggcgcgct gggacgtctg gaagacatcg ccatacagat cgggcttacc
     1621 cagggcaccg cacatcccca gctcagccgt ccctatatca tcgtctttgc cggagaccat
     1681 ggcatcgccg ccacgggcct ggtcaatccc tatccgcagt cggtaaccgc ccagatggtg
     1741 ctgaatttta ttacgggcgg ggcggccatc aatgtctttt gccgccagca tcacctgggg
     1801 ctcaccgttg tggacgcggg gatcaatttc gatttcgagg ccgggctctt ccccgggaag
     1861 ctgatctccg aaaagatcgg ctacggcacc cgcaactatc tcgagaccga agccatcacc
     1921 gccgatgaat acacaaaggc gctggaggcc ggtaagcggg tggtgaccga gctgtacgca
     1981 agcggctgca attgcgtcgg ttttggcgag atgggcatcg gcaatacatc ctccgccgcc
     2041 ctgatcatga gctatattac cgagctgccc gtggaagaat gcgccggaag gggtacgggc
     2101 gtaaacgacg aacaactgcg gacaaagcgg gagaccctgc gaaaagcata tgaacgccat
     2161 ctgccggcga tcaaggcccg gcctggtgcc cagacgatcc tccagtgcgt cggcggattc
     2221 gaggtcgcga tgatgaccgg cgccttcctg aaggcggccg aactgaagat gatcatcctc
     2281 gtcgatgggt ttatcaccac cgcggccctg ttgctggcca aacagctgaa ccccgccgtc
     2341 caggacaatt gtttcttcgc ccatacgagc ggcgagaacg gacatgccgc catgctggat
     2401 tacctcggcg caaagccgtt gttgaacctc gggatgcgcc tgggcgaagg caccggtgcg
     2461 gccatggcct ttcccttgct gcagtcggcc gttctctttc tccgggagat ggcctccttc
     2521 gaatcggcag gtgtaagcaa agcatccccg gatgaaaaat gaatggaaag tattcctggc
     2581 tgccatccag ttcctgacgc ggatacaggt gccggcaggt atcggtcatg agccggcctt
     2641 gctgcaacag gcgccaaaat atttcccgct ggtgggctgg atcgtcggag cgctaagcgc
     2701 cctcttcttc ctggtcttcg cccggtatat ttccaccgat gtcggcatcc tcgcctccat
     2761 gatcaccggt ctgctgatca ccggcgcctt tcatgaggac ggctttgccg atgtctgcga
     2821 tggtttcggc ggaggatgga caaaggaaaa gatcctgctg atcatgaagg acagtcgcat
     2881 cggagcgttt ggggcaatgg gactgatctt tatcctcggg acgaaatttt tgttgttgcg
     2941 ggagctgccc ggctttgtca ccacgccggg tgaggcatca catcttattt tctatcccta
     3001 ccggttcttt atcctcgtgc tgatctcggc gcatagcgtc ag
//
LOCUS       DATGFG010000176         6807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1224875, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000176 DATGFG010000000
VERSION     DATGFG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224875"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VKQ52_03305"
     CDS             <1..896
                     /locus_tag="VKQ52_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HLZ86237.1"
                     /translation="NKPAKKARTREALLAKYIFFNHPLFAEYQEKIIELADTHHHFLL
                     PREGDDRKVTLEGGDVMVVSRDHLLIGVSERTSLEAAHQAIRVLFEKNVVKKITIIRI
                     PKKRDYMHIDTIFTQVKRNVWILLGTFSKKSMLHENADDVQRILDNNRKEDKLKIIQF
                     RKKDPANPEYYENLEDLLTDISKNDLECTEKVRFIYSGNNEFPFDAREQWTDSCNLLA
                     LKEGVVLGYDRNDKTVEAFRAHGFTIVHAKELIAGMEDGTIDPDQVENTLILMPSAEL
                     SRARGGFHCMSMPLHRDDLTI"
     gene            903..1814
                     /locus_tag="VKQ52_03310"
     CDS             903..1814
                     /locus_tag="VKQ52_03310"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HLZ86238.1"
                     /translation="MQTTSHILMIRPVNFTFNAETAVNNAFQVAGADTDAQRKALTEF
                     DGLARLLRDNGVDVTVVDDTALPYTPDSIFPNNWVSFHRDSTVCLYPMYAENRRLERK
                     PGVLDVIGGKFRIDATLDFSPYEQKGLFLEGTGSMVLDRDKKIAYACLSPRTDREVLL
                     DFCRKMDYRPEVFTARDAAGRDIYHTNVMMCVGHRFVVVCLDALPVATEREQLTATIR
                     VSGKAIIPITLEQMNHFAGNMLQVENSKGERLLVMSTQAYESLTAAQLKELTDFSRIL
                     HSPLTTIETNGGGSARCMLAEIHLPPL"
     gene            complement(1707..2234)
                     /locus_tag="VKQ52_03315"
     CDS             complement(1707..2234)
                     /locus_tag="VKQ52_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HLZ86239.1"
                     /translation="MENRATVIVSRRSLVFLRTSIGIIYIWFGALKFFHGYSPAEDIA
                     IQTIDRLTFHLIPEPANIILLATWECLAGLLILTGKMVRTAFFLLFLHMACTFTPLLL
                     FPAASFRYPPYGFTLLGQYIMKNIIIVCAALVLRQADRSQRRKVDLGQHATGAAATVG
                     LDGRQRRMQDAAEVR"
     gene            complement(2352..3269)
                     /locus_tag="VKQ52_03320"
     CDS             complement(2352..3269)
                     /locus_tag="VKQ52_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLZ86240.1"
                     /translation="MEFGKAQYIDNIDFSLPPDDPVTDRLWLQSTPEAGKPLRVHVGG
                     TEWGRSTWVGRVYPRGTKQANFLPFYARQFNTVELNTLFYGLQAPAVIQRWASMVGEE
                     FRFCPKFLETISHKQQLVNAAGDTGSFIDAVRHLGPKLGPSFLQLSEGFGPDRVAVLA
                     NFMRALPRDFRVCVELRHPGWFGPAVSPEIWNLFRELGVGTVITDVAGRRDVLHMRLT
                     APVAFVRFVTNGLHSTDHQRADAWAKRLAAWAANGLEEVYFFVHSPEEQTSPEMMSYV
                     IGRFNETLGAGLHQPQLANGGQPENLSLF"
     gene            3421..4398
                     /gene="pfkA"
                     /locus_tag="VKQ52_03325"
     CDS             3421..4398
                     /gene="pfkA"
                     /locus_tag="VKQ52_03325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HLZ86241.1"
                     /translation="MESKVTKIGVLTSGGDAPGMNAAIRAVVRTGIYYGMEVFGIMRG
                     YSGMIENDIVPMHSRSVANIIQRGGTILKTARCKEFFEHEGRKKAYGNLKKLGINGLV
                     IIGGDGSFRGAHKLSEEFDIPCIGLPGTIDKDIAGTDFTIGFDTAVNTAVEAIDKIRD
                     TADAHDRLFIIEVMGRDAGYIALHSGIATGAENILIPERKTDIEELVSSLLEKERRRK
                     LVNIIVVAEGDRGAEDVANIIKERIPSADTRVCVLGHIQRGGAPTCMDRLIASRMGYS
                     AVESLIEGRHNVMIGIVNNKMHYTPLEKAVKAKQKISEDWLKIVKILAS"
     gene            4426..5919
                     /gene="pyk"
                     /locus_tag="VKQ52_03330"
     CDS             4426..5919
                     /gene="pyk"
                     /locus_tag="VKQ52_03330"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HLZ86242.1"
                     /translation="MSKHLSKYLYQDMDKEAGLQHTFHKTKIVATVGPACDTYDKLLE
                     LVKAGVNVFRLNFSHGTHEDKAKIIEHIRNINISEPYNISILADLQGPKLRVGDIKDG
                     SLAIAPGDILTFTSREKVIGTKEKIYVSYPNLHHDVQVGNKILIDDGKLEVVVVNVSP
                     DGDVKVAVTYGGNLLPKKGVNLPDTKISLPALTEKDLVDLDFIIEQKADWIALSFVRK
                     VEDIIDLKRRLAERNSQSKVIAKIEMPSAMDDLRNIVLESDGVMVARGDLGVELPVEK
                     IPMAQREIIRKCIHRAKPVIVATQMMESMIDRVKPNRSEITDVANAVIEGADAVMLSA
                     ETATGQHPTLVVETMRKIILEVERTEYPYNREDDLVPQPHSPSFLSDAICYNACKLAK
                     DANADALVGMTQSGYTAFILSSYRPRSPLYIFSKEKSLINQLSLSWGVRAFHYDEEVS
                     VDDIIHDQHTILKERGFVKSGDVVVNTGSLPIEEHLPTNMLKITKLK"
     gene            complement(5960..6691)
                     /locus_tag="VKQ52_03335"
     CDS             complement(5960..6691)
                     /locus_tag="VKQ52_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HLZ86243.1"
                     /translation="MDYQLEICAFNLASALLAQQSGADRIELCASPEEGGVTPSPGVI
                     RSAREGLRIALYPIIRPRGGDFLYSDDEFRVMVRDIEYCKQVGCNGVVIGMLNADGSV
                     DQGRSARLVEAAYPLGVTFHRAFDWAANPFEAMETIIQIGCERILTSGQRPTAMEGAE
                     LIDQLVREADDRIVIMPGSGIRAANIVSLAEKTGASEFHTSARLRQASAMNYVNAQMS
                     EAQDVVMAGQDEIRKIKEQLLALQS"
BASE COUNT         1709 a         1972 c         1745 g         1381 t
ORIGIN      
        1 tgaacaagcc ggcaaaaaaa gcaaggaccc gggaagccct gctcgcaaaa tacatcttct
       61 tcaaccatcc cctttttgcc gaataccagg agaagatcat cgagctggca gatacgcatc
      121 accatttcct gctgccgcgc gaaggcgatg accgcaaggt caccctggaa ggcggggatg
      181 tgatggtcgt cagcagggat cacctactca ttggcgtcag cgaacgcacc tccctggaag
      241 cggcgcacca ggccatccgc gtcctcttcg aaaagaatgt ggtcaagaag atcaccatca
      301 tccgcattcc gaagaaacgg gactatatgc acatcgatac catcttcacc caggtgaagc
      361 gaaacgtctg gatcctgctg ggaaccttct cgaagaaatc gatgctgcat gagaacgcgg
      421 atgatgttca gcgtatcctt gacaacaaca ggaaagagga caagttaaag atcatacagt
      481 tccggaagaa ggacccggcc aatcccgagt attacgagaa tctggaagac ctgctcaccg
      541 atatcagcaa gaacgatctg gaatgtaccg agaaagtaag gttcatctac tccggcaata
      601 acgaattccc cttcgatgcc cgggaacaat ggaccgactc ctgtaacctt cttgcattaa
      661 aagagggggt cgtactgggc tatgaccgca acgacaagac cgtggaggcc ttcagggccc
      721 atgggtttac catcgtccat gccaaagagc tgatcgcagg aatggaggat ggaaccatcg
      781 accccgacca ggtggagaat accctgatcc tgatgccttc cgccgaactg tcgcgggccc
      841 gcggcggttt ccattgcatg agcatgcccc tgcatcggga tgacctgaca atttgatcga
      901 ctatgcaaac cacctcccac atactgatga tacggcccgt taatttcacc tttaacgcgg
      961 aaacggccgt gaacaacgct ttccaggtgg ccggcgccga tacggacgcg caacggaagg
     1021 cattgaccga atttgacggc cttgcgcggc tgctgcggga caacggggta gatgtcaccg
     1081 ttgtcgacga cacggccctg ccctataccc cggattccat ctttcccaat aactgggtct
     1141 cctttcaccg ggactccacc gtctgcctct atcccatgta cgcggaaaac cgccggctcg
     1201 aacgcaagcc cggcgtgctg gatgtcatcg gcgggaaatt caggatcgac gccaccctgg
     1261 acttcagtcc ctatgagcaa aaaggtttat ttctcgaagg cacgggaagc atggtcctcg
     1321 accgcgacaa aaaaatagcc tatgcctgtc tgtctccccg taccgacagg gaagtgttgc
     1381 tcgacttctg caggaaaatg gactaccggc ccgaggtctt caccgccagg gacgccgccg
     1441 gccgggacat ctatcacacc aacgtgatga tgtgtgtcgg ccaccggttt gtagtcgtat
     1501 gcctcgatgc cctgcccgtc gcgaccgaac gggaacagct aaccgccacc atccgtgtgt
     1561 ccggaaaggc catcatcccg atcacgctgg agcagatgaa tcattttgcc ggcaatatgt
     1621 tgcaggtaga gaactccaaa ggggaaagat tactcgtcat gtccacccag gcctatgaat
     1681 ccctcaccgc ggcgcaacta aaggagctaa cggacttcag ccgcatcctg cattcgcctc
     1741 tgacgaccat cgagaccaac ggtggcggca gcgcccgttg catgctggcc gagatccacc
     1801 ttccgccgct gtgatctgtc agcctggcgg agcaccaggg cggcacagac gattatgata
     1861 tttttcataa tatattgccc caacagggtg aacccataag gcgggtagcg gaaggatgct
     1921 gcgggaaaaa gcagcagcgg cgtaaaggtg caggccatat ggagaaacag gaggaaaaaa
     1981 gcagtccgca ccattttccc ggttaggatg agcaaacccg caaggcactc ccaggtggcc
     2041 agcaaaatta tattcgcggg ctcgggaata agatggaagg taagtctgtc gatggtttgg
     2101 atagcaatat cctccgccgg actatacccg tggaagaatt tgagcgcccc gaaccagatg
     2161 taaatgatac cgatacttgt acgtaaaaag acgagtgacc tccggcttac aataaccgtt
     2221 gccctattct ccataagcag aaatttttag tacacaaatt tagtagactt acatcgatcc
     2281 cggatgacga ccgtcatcac ccggaatgat cctggtcacc gccgttgcct caaaggatgg
     2341 agagccttct ttcagaacag cgaaaggttt tccggttgcc cgccattggc caattgtggc
     2401 tggtgaagcc ccgcgccaag cgtttcatta aaccgcccga tcacatagct catcatctca
     2461 ggcgaagtct gttcttccgg actgtgtaca aaaaagtaaa cctcctccaa cccattggcc
     2521 gcccaggcgg caagccgttt cgcccaggca tccgcccgtt gatgatcggt tgaatggagc
     2581 ccattcgtca caaaacgaac aaacgccacc ggcgccgtca gccgcatatg cagcacatcc
     2641 ctcctccccg ccacatcggt gatcacggtg ccgacgccca gttcccggaa caggttccag
     2701 atctccgggc tcaccgcggg gccaaaccag ccggggtgcc gcagctccac acagacccga
     2761 aaatcccggg gtaacgcacg cataaagttg gctaacaccg ctacccgatc gggtccaaat
     2821 ccctccgaca attgcagaaa ggaaggcccc agttttggcc ctaaatgccg gacggcgtcg
     2881 ataaacgatc cggtatcccc ggctgcattc accaactgtt gtttgtgact gatcgtttcc
     2941 aggaactttg ggcagaagcg aaactcctct cctaccatcg aggcccagcg ctggatgacg
     3001 gccggcgcct gcaggccata aaaaagggta ttgagttcaa ccgtattgaa ttgccttgca
     3061 taaaagggta agaaattcgc ttgttttgtc cccctggggt atactctgcc gacccaggtc
     3121 gacctgcccc actccgtgcc gccgacatgt acccgcaatg gttttcctgc ctccggcgtc
     3181 gattgcagcc aaagccggtc cgtcaccgga tcatccggcg gcagggaaaa atctatgtta
     3241 tctatatatt gtgctttacc gaattccata gacacttttg attggtcgct tgtggttcaa
     3301 gatcaaatcc ttacttttgg gacttcgatt cgaaaagagc cttaaggtag aaatttttca
     3361 acaaatcctg ccaatggtta aagctttttt aaacctcaaa actaagaact gacttccaag
     3421 atggaaagta aagtcactaa aattggcgta ctcacttccg gcggcgacgc accgggaatg
     3481 aatgcagcca tccgtgccgt tgttcgtacc ggtatttatt atggtatgga ggtgtttggc
     3541 attatgcgtg gttacagtgg aatgatcgaa aacgatatcg tccctatgca ctcccggagt
     3601 gttgcaaata tcatccagcg cggcggcacc atcctcaaga ccgcgcgttg caaggaattc
     3661 ttcgagcatg aaggccgcaa gaaggcctat ggcaatctca aaaaactggg catcaacggg
     3721 ctggtaatca tcggcggcga tggttctttt cgcggcgccc ataaattgag cgaggaattc
     3781 gatatcccct gtatcgggct ccccggcacc atcgacaagg atatcgccgg caccgatttt
     3841 accattggat tcgacacggc cgtcaacacc gccgtggaag cgatcgacaa gatccgggat
     3901 accgccgacg cacacgaccg cctctttatc atcgaggtga tgggtcgtga cgcgggttat
     3961 atcgccctgc acagcggtat cgccaccggc gccgagaaca tcctgatccc cgagcgcaag
     4021 accgatatcg aagagttggt aagctctctc ctcgaaaagg aaagaaggcg caaactggtc
     4081 aatatcatcg tcgtagccga aggcgacaga ggcgccgaag acgtcgccaa catcataaag
     4141 gaacgtatcc cctccgccga cacgagggtc tgcgtgctgg gccatatcca gcggggcggg
     4201 gcgcctacct gtatggacag gcttatcgcc agccggatgg gctattcggc cgtggaatcc
     4261 ctcatcgaag ggcggcataa cgtgatgatc ggcatcgtca acaacaagat gcactataca
     4321 cccctggaaa aagcagtaaa agccaaacaa aagatcagtg aagactggct gaaaatcgtc
     4381 aaaatattag cgtcttaata agcgtcttaa aaaaatccac cgtcaatgtc aaagcatcta
     4441 tcgaagtact tgtatcagga tatggataaa gaagctggtc tccagcacac ttttcataaa
     4501 acgaagatcg tcgctaccgt aggccccgca tgtgacacct atgataaatt actggaactg
     4561 gtaaaagccg gcgtcaatgt ctttcgcctc aatttctcac acggcacaca cgaggacaag
     4621 gcaaagatca tcgagcacat ccgcaacatc aacatctccg agccctataa tatttccatc
     4681 ctggctgacc tgcagggacc caaactgcgg gtaggcgata tcaaggacgg aagcctcgcc
     4741 atcgcccccg gcgatatcct cacctttacc tcgcgggaaa aggtgatcgg gacaaaggaa
     4801 aagatctatg tctcctatcc caacctgcac catgacgtac aggtaggcaa taagatcctc
     4861 atcgacgatg gaaaactgga agtcgtcgtc gtgaatgtat cccccgatgg cgacgtaaag
     4921 gttgccgtca cctatggcgg gaacctgctg ccgaaaaaag gggtcaatct tcccgatacc
     4981 aagatctccc tgccggcact caccgaaaag gacctggtcg acctggactt catcatcgag
     5041 caaaaggcgg actggatagc gctctccttt gtccgtaaag tagaggatat cattgatttg
     5101 aaacggcggc ttgccgagcg taacagccag agcaaggtga tcgcaaagat cgagatgcct
     5161 tcggcgatgg acgacctccg caacatcgtc ctggaatccg acggcgtcat ggtagcacgc
     5221 ggcgacctcg gcgtcgaact gcccgtcgaa aagatcccga tggcgcagcg tgagatcatc
     5281 cgcaagtgta tccaccgcgc caagcccgtc atcgtcgcca cccagatgat ggaaagtatg
     5341 atcgatcggg taaagcccaa ccgcagtgag atcaccgacg tcgccaatgc cgtgatcgaa
     5401 ggcgcagacg ccgtcatgct cagcgcggaa acggccaccg gtcagcaccc gacccttgtc
     5461 gtggagacca tgcgcaagat catcctggaa gtagaaagaa cagaataccc ctataaccgg
     5521 gaagacgacc tggttcccca gccgcattcg ccttccttcc tcagcgatgc catctgttat
     5581 aacgcctgca aactggccaa ggacgcaaat gccgacgccc tcgtcgggat gacccagagc
     5641 ggctatacgg ccttcatcct ttcgagctac cggccgaggt ccccgctgta catcttctcc
     5701 aaggagaaaa gcctgatcaa tcaactgagc ctcagctggg gtgtccgcgc cttccactac
     5761 gacgaagagg tcagcgtgga cgatatcatc cacgatcagc atacgatcct gaaggaacgc
     5821 gggtttgtaa aatcgggaga tgtggtggtc aataccggca gtctgcccat cgaagagcac
     5881 ctgcccacga atatgctgaa gatcacaaaa ctcaaataga cgggctccgc ctgatgcgat
     5941 agttataaga acagggcagc taactctgca atgcgagtag ctgctctttt atttttcgga
     6001 tctcgtcctg tcccgccatc accacatcct gggcctcgct catttgggcg ttgacatagt
     6061 tcattgcgct ggcctggcgc aaccgtgcgg aggtgtggaa ttcggaggcg ccggtcttct
     6121 ctgcgaggct gacgatattc gcggctctta ttcccgaacc gggcatgatc acgatccggt
     6181 cgtcagcctc ccttaccagc tggtcgatca actccgcgcc ttccatcgcc gtcggccgtt
     6241 gaccggaggt caggatgcgt tcacacccga tctggatgat cgtttccatg gcctcgaagg
     6301 gattggccgc ccagtcaaaa gcgcgatgaa aggtcacgcc cagcgggtaa gccgcttcca
     6361 ccagccgggc tgagcgcccc tggtcgaccg agccatcggc gttcagcatg ccgatgacga
     6421 cgccgttaca ccctacctgc ttacaatatt cgatgtcccg gaccatcacc cggaactcat
     6481 catcggaata caggaaatcc cctccccgcg gccggatgat gggatagagc gcgatccgca
     6541 aaccttcccg ggcagaccgg ataacgcccg gagaaggtgt gacaccaccc tcctccggcg
     6601 aggcgcaaag ctcgatacga tcggccccgg actgctgcgc caacagggcg gaagcaagat
     6661 tgaaggcgca aatctccaat tggtagtcca taatcagaaa tagcttttta catccaccat
     6721 tctctcttct ttattgttct ttatcgcata ggaaaagcct ttttgtattg cataggcccc
     6781 cgtttcccct tttttgttca gcgcgat
//
LOCUS       DATGFG010000177         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1107504, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000177 DATGFG010000000
VERSION     DATGFG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107504"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..>2410)
                     /locus_tag="VKQ52_03340"
     CDS             complement(40..>2410)
                     /locus_tag="VKQ52_03340"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HLZ86244.1"
                     /translation="PEGATATANAAHGATLVFSMVGYVSQTVAVASVKDSLVIALSPD
                     AQKMQQVVVTNRREGKYRNKDNPAVELIRKVIANKDNNRPESFDHASYEEYDKVEISM
                     RDIDSTKFHKRLLRPYRFLFEHPDTVAGDTSLYYPIYLEEKLSDNYFQKKPERRKQVV
                     KAEKKVDFGDLIDTKGLSSYVNTLLSDVDIYDNNILLFTNQFLSPMANAAPTYYEFFL
                     GDTVVVNGERLVRLNFQPRNNDDLLFRGMFYITLDGNYAVQRVQLTLSKRVNLNFIKN
                     LHVRQEFQRTPNGHYYRVVSDASADFSLSAKKKGLHGRKYVAFAKLQTEKPIADSVFR
                     QATAAEPSPEALSRSNAYWDEHRQEPLSSGESLVYANIDSLRRLRTFIRTKDLINLVA
                     AGYKSAGPFEIGPVNTFYSFNPIEGFRLAFGGRSTPLLSKRIWLDGYGAYGFGDRRWK
                     YDAGVAYSFNRLSDYGYPQHYLKFSYQHDVKIPGQELQFVQDASFLASFKRGVNDVYL
                     YNDIFRIDYLRELPNHLSYGFGFKYWAQQPAGAIEYVQPKLTISDTLTNLVTSQLSAQ
                     IRWAPHEKFYQGRVYRIPFPNQYPVFSLQFIAGIKGLAGGNYSYQDVNLNIFKRFYLS
                     TFGYTDVTLSGAYVAGKLPFPLLQILPANQTYIYQPDSYNLMNFLEFVSDHYAGMDVE
                     HHFNGFIFNKIPVLKRLKWREILTAKLLYGGLRAENDPSKNASAMRFPLIDGQASTFA
                     LGNQPYLEAGFAIGNIFKLLRVDFIERFSYLNHPNVPQYGVRFQVKFDY"
BASE COUNT          580 a          682 c          609 g          539 t
ORIGIN      
        1 agataataca tacttcccct taaaggtagg ccaaaattcc taatagtcaa acttcacctg
       61 gaagcggacc ccgtactgcg gcacattcgg atggttcagg taagagaacc gttcgataaa
      121 atccacccgc aatagtttaa agatatttcc aatagcaaac cccgcctcga gatagggttg
      181 attacccagt gcgaacgtac tcgcctgccc atcgatcagg ggaaaccgca tcgccgaagc
      241 gttcttcgat ggatcgttct ccgcccgcaa ccctccgtac agcagcttcg cggtgaggat
      301 ctccctccac ttcaaccgct tcaacaccgg gatcttgtta aaaataaatc cattgaaatg
      361 atgctccaca tccatgcccg cataatgatc actcacgaat tccaggaaat tcatcaggtt
      421 ataggaatcc ggctggtaaa tataggtttg attcgcgggc agtatctgca acaacggaaa
      481 aggtagcttg cccgcgacat aggcccccga caacgtgaca tcggtataac cgaaggtcga
      541 caggtaaaac cgcttgaaaa tattcagatt gacgtcctgg taggaatagt tgcctccggc
      601 cagccccttg atgcccgcga tgaattgcag gctgaacacg gggtattgat tcggaaaggg
      661 gatccggtat acccttccct ggtagaactt ctcatgagga gcccaacgta tctgcgcaga
      721 caactggctg gtcaccaggt tggtcagggt gtcggaaata gtcaatttcg gctgtacata
      781 ctcgatggct cccgcaggct gctgggccca atacttgaag ccaaaaccat aagagagatg
      841 gttcggcaat tcccgcaggt agtcgatccg gaagatatca ttgtacaggt agacatcgtt
      901 gacaccccgc ttgaaggaag ccagaaaact ggcgtcctgc acgaattgca gctcctgccc
      961 ggggattttc acatcatgct ggtagctgaa cttcaggtag tgctgcgggt agccataatc
     1021 agaaagcctg ttgaaggaat aggcgacgcc cgcatcgtat ttccaccgcc ggtcgccgaa
     1081 cccataagcg ccataaccat ccagccagat gcgcttgctc agcaacggag tgctccgtcc
     1141 gccaaaagcc agccggaatc cctcgatggg attgaaacta taaaaggtat tcaccggccc
     1201 gatctcgaac ggccccgcgc tcttgtaccc cgccgcgaca aggttgatga gatcctttgt
     1261 ccggataaac gtgcgaagcc ggcgaaggct gtcgatattg gcatagacca gcgactcccc
     1321 ggaggaaagc ggctcctgcc ggtgctcgtc ccaatatgca ttactcctgc tcaacgcttc
     1381 gggtgagggc tccgcggcgg tggcctgccg gaatacgctg tcggcaatcg gcttctctgt
     1441 ctgcaacttc gcaaatgcca catatttccg cccatggagc cccttctttt tggcagagag
     1501 cgagaaatcc gcggaagcat ccgaaaccac ccggtaataa tggccgttgg gggttcgttg
     1561 aaactcctgg cggacatgca gattcttgat gaaattgaga ttgacccgtt tgctgagcgt
     1621 caactgcaca cgttggaccg catagttccc gtcaagggta atatagaaca tcccccggaa
     1681 cagcaggtcg tcattgttcc gcggctggaa gtttagcctt accagccttt cgccgttcac
     1741 aaccaccgta tcgcccagga agaattcata atacgtaggc gcggcattgg ccatcgggct
     1801 gaggaattgg ttcgtgaaca gcaggatatt gttgtcgtag atgtccacat ccgacaacag
     1861 ggtattcaca tagctgctca accccttcgt atcgatcaga tccccgaaat ccaccttctt
     1921 ctccgccttg accacctgct tgcgccgctc cggcttcttc tggaaatagt tatctgaaag
     1981 cttttcctcc aggtagatcg ggtaatacag ggaggtatca cccgccaccg tatccgggtg
     2041 ttcgaagaga aaacgatagg gacgcagcag ccgcttgtga aatttcgtgg agtcgatatc
     2101 ccgcatactt atctccactt tatcatattc ttcatacgaa gcatgatcaa acgactccgg
     2161 ccggttatta tccttattgg cgatgacctt ccggatcagt tccaccgccg gattatcctt
     2221 gttgcggtat ttcccctcgc ggcggttcgt gaccaccacc tgctgcatct tctgcgcgtc
     2281 gggggagagg gcaatgacga gactatcctt cacggaagca accgccaccg tctgcgaaac
     2341 ataacccacc atcgagaaca ccagggtggc gccgtgcgcg gcgttggcag ttgcggtggc
     2401 gccctccggc
//
LOCUS       DATGFG010000178         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_998016, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000178 DATGFG010000000
VERSION     DATGFG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998016"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VKQ52_03345"
     CDS             <1..1307
                     /locus_tag="VKQ52_03345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLZ86245.1"
                     /translation="HTLYSTSKSFTAAAVGFVLTEKKLKLTDKLVSFFPPEELPNPVP
                     PFLTELTVKDALMMSDGMDPDPSGPVGMSADWAKAFFATPIVNKPGTKFLYNSAGTYM
                     LSAVVQKVTGEKILDYLRPRLFDPLGITGEDWETSPQGVNTGGWGLRLKTEDMAKFGQ
                     LYLQGGRWKGKQILPHDWVKEATTMKIMQDPSASPDKQDASDWLQGYCYQMWRCRHNG
                     VRADGAFGQFIIMLPDQDAVIAITAETPDMQDEINLVWQYLLPPMVGWSLKEDPDGDA
                     KLKQTLAALALPPQKAAATGADFNINGKMYTLQANDKRMAAIGFRLQDDNSWQVTIKG
                     DSAEYSFPFGDGKWLRTSTTRLGPNLTDHAQHHFAGMPAVMTAGSYHWKDENTLELVL
                     RYIESPHTEKMTCHFDGSTVSIDDEASFDYGKKKVAMKGTLQ"
     gene            1545..>3173
                     /locus_tag="VKQ52_03350"
     CDS             1545..>3173
                     /locus_tag="VKQ52_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HLZ86246.1"
                     /translation="MQKLSFLLPAALCAMSMARVCAQNVGIGTTHPQQKLHVAGGLRV
                     DTLSNGRDSGLLRHSADGTVFSLVFSGDSTQVLRGNGTFGTAAAASGAVTPFGLVTDP
                     WLLGGNAGVTPATNFLGTTDNAALRLRINNQWAGQLDSVSQSTFFGYGAGKNDTAVAA
                     GNTAVGYQALSVVTGGTFNTATGVRALLNNSTGVYNTATGWQAMSQNTSGAQNTALGV
                     QALQNNTTGGNNTAVGLNSLNVNSTGSNNTAVGVQALNFNTQGNFNTAIGRSALFANS
                     TGNENVASGHLALAGNSAGSANVAVGSNALRSCGSCTETVAIGDSALFTQTQFSNPNQ
                     IGFANTAIGSKALFSNTTGPFNTVVGYQSLTSNTTGFDNIVVGALSMPVSTTAGENIA
                     IGNVVLFHNTSGSNNVAIGPAALNNNITSFDNTALGDGADIAMDGLFNITSLGSGAVA
                     SSSNSVMLGDAAVTAVNTFGSFHTISDGRFKYNIREDVKGLDFILKLRPVSYQLDVKQ
                     ANDHLRKGMVPIVSTNLRREDRGTQMRAAAFTEAS"
BASE COUNT          677 a          768 c         1080 g          648 t
ORIGIN      
        1 agcacacctt gtattcgacg agcaagagtt ttacggcggc ggctgtgggg tttgtgctga
       61 ccgagaagaa gttgaaactt accgataagc ttgtttcctt ttttccaccc gaggaactgc
      121 cgaaccccgt gccgcccttt ctgacggagc tgacggtgaa ggatgcgctg atgatgtcgg
      181 atgggatgga ccccgacccg agcgggccgg tggggatgtc cgcggattgg gcgaaagcct
      241 tttttgcgac gccgatcgtg aataagccgg ggacgaaatt cctgtacaac agcgcgggga
      301 cgtatatgct gtcggccgtc gtgcagaagg tgacgggtga gaagatcctg gattatctgc
      361 ggccgcggct tttcgatccg ctcgggatca cgggagaaga ctgggagacg agcccgcagg
      421 gagtgaatac cgggggctgg ggtttgcggc tgaagacgga ggatatggcg aagttcgggc
      481 agctgtattt gcagggcggc cgctggaagg gaaagcagat actgccgcac gactgggtga
      541 aggaggcgac gacgatgaag atcatgcagg acccgagtgc gtcgcccgac aagcaggatg
      601 ccagcgactg gttgcaggga tattgttacc agatgtggcg gtgccggcac aacggggtgc
      661 gggcggatgg ggcgtttggg cagttcatca tcatgctgcc ggaccaggat gccgtgatcg
      721 cgatcacggc ggagacgccg gatatgcagg atgagatcaa tctcgtctgg cagtacctgt
      781 tgccgccgat ggtaggctgg tcattgaagg aggacccgga tggggatgcg aagctgaagc
      841 agacgctggc ggcgctggcg ctgccgccgc agaaggcggc ggcgacggga gcagacttca
      901 atatcaacgg gaagatgtat acgctacagg cgaatgataa gagaatggcg gcgatagggt
      961 tccggctaca ggacgacaat tcctggcagg tgacgatcaa gggggattcg gcggagtatt
     1021 ccttcccctt tggggacggg aagtggctgc ggacctcgac gacgaggctg gggccgaacc
     1081 tgacggatca tgcgcagcat cattttgcgg ggatgccggc ggtgatgacg gcggggagtt
     1141 atcattggaa ggacgagaac acgctggagc tggtgctgcg gtatatcgag agcccgcata
     1201 cggagaagat gacctgtcat ttcgatgggt cgacggtgtc gatcgatgac gaggccagtt
     1261 ttgactatgg gaagaagaag gtggcgatga aggggacgct gcaatagggc cgggtcaggc
     1321 gttggagcgg gaaggtgtct tcgtccgacg gccggggtgc cgctgcaata ggtatttggt
     1381 gcagcgatgt tcgtggcgac cggttttcag gcgacaaggg ccgcatcggg gagttcagcg
     1441 cacgcatagc gcagggaata aaaaaaatac cctcattagg gtaatgggcc ggctacatac
     1501 cggcccattt tttgtgtctg atccaacaca ctaaaccctt taccgtgcaa aagctttcct
     1561 ttttgctccc ggccgcgtta tgcgccatga gcatggcgcg ggtatgcgcc cagaatgtcg
     1621 ggatcggcac tactcatcct caacaaaaat tacatgtggc aggcggcctg cgggtcgaca
     1681 ctttatccaa cggcagggac agcgggctgt tgcggcatag tgccgacggc accgtcttca
     1741 gtcttgtctt cagcggcgac agcacgcagg tgttgcgcgg caatgggacg ttcgggaccg
     1801 cggcggcggc atcgggagcg gtgacgccgt ttgggttggt gacggacccg tggctgctgg
     1861 gcggcaatgc gggggtgaca ccggccacga attttctcgg caccacggat aatgcggcgc
     1921 tgcggttacg gatcaataac cagtgggcgg gccagttgga ttcggtgagc cagtccacgt
     1981 tcttcggtta tggagcgggt aagaacgata ccgctgttgc ggcgggcaat actgccgtcg
     2041 gttaccaggc tttgagcgta gtgaccggcg gcacgttcaa tacggctacg ggcgttcggg
     2101 cgctgctgaa taattccacg ggtgtctaca atacggccac aggatggcag gcgatgtccc
     2161 aaaacacgtc gggtgcacag aacacagcct tgggtgtaca ggccctgcag aataatacga
     2221 cgggcggcaa taatacggcc gtggggctga actctttgaa tgtgaactcg acgggttcga
     2281 ataatacggc ggtgggtgtg caggcgctga atttcaatac acaggggaat ttcaatacgg
     2341 ccatcggccg atcggcgttg tttgccaatt cgacgggcaa cgagaatgtt gcctcggggc
     2401 atcttgcgct ggccggtaat tccgcggggt cggcgaatgt ggctgtgggc tccaatgccc
     2461 tgaggagttg tggcagctgc acggagacgg tggcgatcgg ggattcggcc ttgtttacgc
     2521 aaacgcaatt ctccaatccc aaccagattg gctttgccaa cacggcgata ggcagcaagg
     2581 cgctgttttc gaacacgacg ggacccttca acacggtggt ggggtatcaa tcgctgactt
     2641 cgaatacaac aggctttgac aatattgtcg tcggggcctt gtcgatgcct gtcagcacta
     2701 ccgcgggaga gaatatcgcc attggaaatg tggtcctgtt tcataacacg tccggcagca
     2761 acaacgtggc catcgggccc gctgccctga acaacaacat caccagcttt gacaataccg
     2821 ccctgggaga tggggcggat atagcgatgg acgggctttt caatattacc tctcttggat
     2881 ccggggcggt agcgtcgagc agtaatagtg ttatgctggg cgacgccgcc gtgacggcag
     2941 tgaatacctt tggcagtttc catactattt ccgacggccg gttcaagtac aatatccggg
     3001 aagatgtaaa ggggttggat tttatcctca agctgcgacc ggtgagctat caattggatg
     3061 ttaaacaggc gaatgatcat ctccgtaagg ggatggttcc tattgtctca acgaacttgc
     3121 ggagggaaga ccgggggacg cagatgcggg cggcggcttt taccgaagct tcg
//
LOCUS       DATGFG010000179         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1107258, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000179 DATGFG010000000
VERSION     DATGFG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107258"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VKQ52_03355"
     CDS             <1..1059
                     /locus_tag="VKQ52_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLZ86247.1"
                     /translation="AINMAIRQRRMEKEKQEAFEAMRITNERFQILSRATRDAVWDWN
                     LSSGWVWGNEAFFELLGFDARQKVPPPTEWGRHVHPADQAKLTARIRGIKNSRISGWE
                     DQLRFRSLSGHFKTVLDRAYVIRNDDGKPDRVVGVFVDVTNKIEQQKLITRTILEVQE
                     KERNLIGRELHDNINQILASVNLKLGYYLEEPLGNEEIIDLCRQNLRKAIDEGRKLSH
                     DMVMPRFSERRLRDEIELLIDDYHYSHIAWLNSEGLDETALPHHIKETLFRIAQEHMN
                     NIHKHAKASSIEIKLVNSPHEVSLYIRDNGVGFNTKQNRKGIGITNILSRAESYDGIA
                     RILSAPGKGCTLSVNIPY"
     gene            1118..1720
                     /locus_tag="VKQ52_03360"
     CDS             1118..1720
                     /locus_tag="VKQ52_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86248.1"
                     /translation="MGEFLHIALIREFTVCAPPARSPRTSLDRFKSAVAALVVDSPDS
                     FDCVEEAGILRWTWKRDVRQAWLTGLLNRYYADFYDAGSDFFLANCKPVLGFLAGAPS
                     DEEWLDWMEKEGSEAFYPMNGSFRTIVLDCKGIQVQVSLVSLSSEGKVRLEESQGHLP
                     FFEKLLRRAYAGNPLAGCLTFLINTSQPPQFSARPRSGTN"
     gene            complement(1662..2807)
                     /locus_tag="VKQ52_03365"
     CDS             complement(1662..2807)
                     /locus_tag="VKQ52_03365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLZ86249.1"
                     /translation="MQQPSRVPWIDYLRGFITLLVVAHHSSLAYTTFAHFNPTAYIAS
                     THPVVDTIRWRGLDIFEDFNDIFFMSLMFLISGIFVMQSIAKKGTALFIRDRFHRLFI
                     PFAIGVTVLMLTAYYPAYLLAHGRHDIKAYVIDFFTVESWPVGPPWFIWVLFLFNLLF
                     ALTYRFTQGWLTKAGAVMAVLKRHPARLVAAWYILTWIVYVPAVLLVGPSVWTGIGPF
                     DFQVSRILLYLGYFLLGIAVGGTGLPYRRWPFWLIGCLSVYALLKWSEAPIGRLRDQH
                     TLSVLQARLIYRSLWTLSCTLSCITFLTVFKNLFKRSRAWWDSLSANAYGIYLIHYIF
                     VLWCQYFLLDAALPAVVKFLITFILALTVSWSLTWAVRKIGVVAKYL"
     gene            3096..3701
                     /locus_tag="VKQ52_03370"
     CDS             3096..3701
                     /locus_tag="VKQ52_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ86250.1"
                     /translation="METLSTDVERLIKDTAKRIFFVEGNLHATTQDIADAAGINRASI
                     HYYYRSRKLLFDKVFEEAMEEFRSKLSVVIGQDGLPFREFLNKLLDFLLKKSMEHPFL
                     ELFLVAELNTNPDMNRSFLPEAAQERKILLKEYIAAEVAAGTMKPIEPEHFIVNLMSL
                     CSFPFLAKPIVKHAIDLSEEAYVKFIGERKEVILKQLFLDR"
     gene            3775..>4645
                     /locus_tag="VKQ52_03375"
     CDS             3775..>4645
                     /locus_tag="VKQ52_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ86251.1"
                     /translation="MKKSRLLFILALAMSLTGTAQRPYTLQECIDTALQKSLQLKSDN
                     YDLAKTQAGIGEAYSSLLPSVNASAGYQYYFQLQKQLIPADLFGGTAGKYTATSLSTA
                     QSKSLGIQLNQTLFNASSLIALKAAKVAVNLNELQIRSSREDLVYNVSATYYNIQSIL
                     MQQAMVRKNLSIDESLVKVTADQLRAGLATQTDLDRLTVQRDNTRATLQNTSNSLEKQ
                     YNMLRLLMNVPLETDIAVLQEEYDEEEALASQQKTDVDLTGKTNYRQVLENKKVADLQ
                     RRNIKAGYLPSLSL"
BASE COUNT         1304 a         1259 c         1161 g          921 t
ORIGIN      
        1 gcgataaata tggccatcag gcaaagacgc atggaaaaag aaaaacagga ggcattcgaa
       61 gcgatgagga tcacgaacga acggtttcag atcctgtccc gggccaccag ggacgctgtc
      121 tgggattgga acctctcctc cggctgggtt tggggtaacg aggccttctt cgaactgctg
      181 ggattcgacg ccaggcagaa agttcctccg ccaaccgaat ggggaaggca tgttcatccg
      241 gcagatcagg caaagctgac ggccaggatc cgcggcataa agaacagcag gatctccggt
      301 tgggaggatc agcttcggtt ccgttccctg agcggccatt tcaagaccgt gctggacagg
      361 gcatacgtga tccggaacga tgacggaaag ccggaccgcg tcgtcggcgt cttcgtggat
      421 gtcaccaaca agatcgaaca acaaaaactg atcacaagga cgatcctcga ggtccaggaa
      481 aaagagcgca atttgatcgg ccgggaactg catgacaata tcaaccagat cctggcttcc
      541 gtaaatctga aactgggcta ttacctggaa gaacccctgg gcaacgaaga gatcatcgat
      601 ctgtgccggc aaaacttacg gaaagccatc gacgaaggcc gtaaactgtc ccatgatatg
      661 gtcatgccgc gcttttccga acgccgcctg cgcgacgaga tcgaattgct gatcgatgac
      721 tatcattata gccatatcgc ctggttgaat tcagaaggcc tggatgaaac ggcccttccc
      781 catcatataa aagagaccct gttccggata gcgcaggaac atatgaacaa tatacataaa
      841 cacgcaaagg caagcagcat cgagataaag ctggtcaaca gcccccatga ggtgagcctc
      901 tatatcaggg ataatggcgt tggattcaat acaaaacaaa acaggaaagg catcggtatc
      961 accaacatcc tcagcagggc cgaatcctac gacggaatag ccaggatcct ctcggcgccg
     1021 ggaaagggct gtaccctgtc tgtcaatatt ccttattgat ttaccccacc cgttggttat
     1081 ttccccgcag aaactgttta tagatataaa ctgcagcatg ggagaatttt tacatattgc
     1141 ccttatcagg gaatttaccg tatgcgcgcc cccggcaaga tcgccacgca cctcgctgga
     1201 cagattcaag tcggccgtgg ctgcattggt ggtggacagc cctgacagct tcgattgcgt
     1261 ggaggaggcc ggtatcctcc ggtggacatg gaaaagggat gtccggcagg cctggctcac
     1321 ggggctgcta aaccgctact atgcggattt ttatgatgca ggaagtgatt ttttcctcgc
     1381 taattgcaag cccgtgctcg ggttcctggc cggcgctccc tcggatgagg aatggctgga
     1441 ttggatggaa aaggagggta gtgaggcatt ctatcccatg aacgggtcgt tccggacgat
     1501 cgtactggac tgtaagggta tccaggtaca ggtttccctg gtctccctaa gctccgaagg
     1561 gaaggtcagg ctggaagaaa gccaggggca tctccccttt ttcgaaaaac tactacgcag
     1621 ggcctatgcc ggtaacccgc tggccggatg cctcaccttt cttataaata cttcgcaacc
     1681 accccaattt tccgcacggc ccaggtcagg gaccaactga cggtcagcgc caggataaac
     1741 gtgatcagga actttaccac ggccggcaga gccgcatcca atagaaagta ttggcaccaa
     1801 agcacaaaaa tataatgaat gaggtagatc ccatacgcgt tggcggaaag ggagtcccac
     1861 caggcgcggg aacgcttgaa gaggttcttg aacaccgtta gaaaggtaat acagctcagg
     1921 gtgcaggata gcgtccacag ggaccggtag atcagccgcg cctgtagtac actgagggta
     1981 tgctgatcgc gcagccggcc gatcggcgct tcgctccact ttaagagcgc atatacagaa
     2041 agacaaccga tcagccagaa cggccatctc ctgtagggaa gccctgtgcc gccgaccgct
     2101 attcccagga gaaaataacc gaggtacaat agtatcctgc tcacctggaa atcaaacggc
     2161 ccgatacccg tccacaccga aggccccacc aaaagcaccg cgggcacata tactatccaa
     2221 gtaaggatat accaggcggc caccagcctc gccggatgcc gttttagtac ggccatcacc
     2281 gctcctgcct tggtcagcca cccctgcgta aagcgatagg tcaaagcaaa aagcaggttg
     2341 aataaaaata atacccagat aaaccagggt ggcccgacag gccaggattc caccgtgaaa
     2401 aaatcgatga catacgcttt gatatcatgc ctgccatggg ccagcaaata agccgggtaa
     2461 taggccgtta gcatcagcac cgtaaccccg atcgcaaagg gaatgaacag gcggtggaac
     2521 cggtcccgga taaacaaggc ggtccctttt ttagcgatgc tttgcatgac aaagatcccg
     2581 ctgatcagga acatgagaga catgaagaag atatcgttga agtcttcgaa gatatccagc
     2641 ccccgccaac ggatggtatc gaccacagga tgggtggatg caatgtaggc ggtcggattg
     2701 aaatgagcga acgtggtgta ggctaaagaa gaatggtggg caacaacaag cagggtgatg
     2761 aacccgcgca aatagtcgat ccaggggacg cgggaaggtt gttgcattcg gtcggccttt
     2821 tccctggtac gattgaagcc ggcagatgtt acaaaactat ccgcaacccc caacccatcc
     2881 gcaaaggccc cagcctatcc acaaacaccc caacctatcc gcaaccaccc ccgggccgaa
     2941 actcctcaac ctggccgcaa atggcccttc ctgccccctt tcgaaaaaaa atttgcccca
     3001 ttgaaaaaag tttctacttt tgtgttgaac aaaattgtta aacacgctgt caacggtaac
     3061 taaacccgcc gacaacggtt gttaacaggt cgaaaatgga aactttatca actgacgttg
     3121 aaagacttat taaggacacc gcaaaaagga tcttttttgt ggaaggcaac ttgcacgcca
     3181 ccacccagga tattgccgat gccgccggga tcaaccgtgc ttccatacac tattactacc
     3241 gaagccggaa acttctcttc gacaaggtat tcgaagaggc aatggaggaa tttcggtcga
     3301 aattgagtgt tgtcatcggc caggatgggc tgccgttcag agaatttctg aacaagctgc
     3361 ttgacttttt gctcaaaaag tcgatggaac accccttcct ggaactattc ctggtggccg
     3421 aactgaacac gaaccccgac atgaatcgct ccttcctgcc ggaagccgca caggagcgaa
     3481 aaattctgtt gaaggagtat atagccgccg aagtggcagc cggcacgatg aaaccgattg
     3541 aaccggaaca ttttatagta aatctcatgt ccttatgctc cttccccttc ctggcgaaac
     3601 cgatcgtgaa gcatgcgatc gacctgtcag aagaagcgta cgtgaaattt ataggggaaa
     3661 gaaaagaggt cattctgaaa caactttttt tggatcggta aagggaacat tttttttgtc
     3721 tccgatctaa caaaattgtt aaacataaaa acaaaacaat ataattaaat aaaaatgaaa
     3781 aaatcccgcc tcctattcat cctcgccctg gccatgtccc tcaccggcac tgcccaacgg
     3841 ccctacaccc tgcaggagtg tatcgacacc gcccttcaaa aaagccttca gctgaaaagc
     3901 gacaactacg acctcgccaa aacccaggcc ggcatcgggg aggcctacag cagcctcctg
     3961 ccctcggtga acgcaagcgc cggctatcaa tattattttc agctgcaaaa gcagttgatc
     4021 ccagctgacc ttttcggggg aaccgccgga aagtacacag ccacatccct gagcaccgca
     4081 cagagtaaat ccctgggcat acaattgaat caaacccttt ttaatgcgtc ctccctcatc
     4141 gcattaaagg ccgctaaagt agccgtcaat cttaacgaac tacagatccg cagcagcagg
     4201 gaagacctgg tatacaatgt ctcggccacc tactacaaca tccaatccat tctaatgcaa
     4261 caggcgatgg tccggaagaa cctttccatc gacgagtccc tggtaaaagt gaccgccgac
     4321 cagctcaggg ccggcctcgc cacccaaacc gacctggaca ggcttaccgt ccaacgggat
     4381 aataccaggg ccaccctgca gaataccagt aacagcctgg aaaaacagta caatatgctt
     4441 cggctgctca tgaatgttcc cctggaaacc gatatcgccg tgctgcagga ggaatatgac
     4501 gaagaagaag ccctcgcgag ccagcaaaag acggatgtcg acctcaccgg gaaaacaaac
     4561 taccggcagg tgctggaaaa caaaaaggtc gcggatctgc aacggcgcaa tataaaggcc
     4621 ggctatctcc cctccctctc actgg
//
LOCUS       DATGFG010000180         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1338893, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000180 DATGFG010000000
VERSION     DATGFG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338893"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VKQ52_03380"
     CDS             complement(<1..211)
                     /locus_tag="VKQ52_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86252.1"
                     /translation="MDIQQIPQADLLDILFEGRNKDYGAYDLRKSYNSRLNRSILSTL
                     AICLLLFIGYTVAGKTHHTISRQAIP"
     gene            complement(459..779)
                     /locus_tag="VKQ52_03385"
     CDS             complement(459..779)
                     /locus_tag="VKQ52_03385"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLZ86253.1"
                     /translation="MTEAEKKHLNQWIEKAEHDLIAAKLIVEQQPLILDIACFHCQQA
                     VEKYLKTYLVFKKEEFPKTHSLDLLQQMCSNYEAAFAGIDLKNMEDFAVRRRYPHDFI
                     SPEL"
     gene            complement(776..1093)
                     /locus_tag="VKQ52_03390"
     CDS             complement(776..1093)
                     /locus_tag="VKQ52_03390"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLZ86254.1"
                     /translation="MNAQQTFQAIKSTVYSFLPNSKVLLFGSRARGQADKQSDYDLLI
                     VTNDTFAPRVKMDWENKIRKALVYALKAPFDVIVESEDEVKEKKELTGHIVHYAMKEA
                     VEI"
     gene            complement(1156..>2330)
                     /locus_tag="VKQ52_03395"
     CDS             complement(1156..>2330)
                     /locus_tag="VKQ52_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLZ86255.1"
                     /translation="SMTINATGYILLAFYAALAKQQGADWKKITGTIQNDILKEYAAR
                     GTYIYPPKPSMHIITDIFEWCSRELPRWNTISISGYHIREAGSTAVQEIAFTLSNGKA
                     YVQAALEKGLDIDVFGKRLSFFFNAHNNLFEEVAKFRAARRMWARIMQELGATDPKAM
                     MLRFHTQTGGSTLTAQQPLNNISRVTVQTLAAVLGGTQSLHTNGYDEALSLPTEEAAR
                     IALRTQQVVAYESGAADTADPLAGSYYVEALTREVEEAAWKLIEHIDRMGGSVSAIEQ
                     GFIQDEIARSAYEYQQRVESKEKIIVGVNSFPTEEKDSIPVFRIDDSIRTVQIEKLND
                     LKRRRDPAKVDSVLQDLNDKAASGENIMPVVVEAVERRCTLGEIADTLREVFGEYK"
BASE COUNT          515 a          597 c          595 g          623 t
ORIGIN      
        1 cgggtattgc ctgccgtgag atcgtgtggt gcgtcttccc tgccacggta taaccgatga
       61 acagcaaaag acagatggca agtgtactca atatactccg gttcagccgg ctgttatagg
      121 acttgcgcag atcgtaggcg ccatagtcct tattgcgacc ttcgaacaag atatcgagca
      181 ggtcggcctg gggtatttgc tggatgtcca taagcgaatg tttctaatga gacacccatt
      241 gctgcagaat ccacagccat cgcacccgct atatttgttt gtggaaaagc gatatttgcg
      301 catcgctatc gcaaatgact ggtcaatcta atatcggcag atataccttc caaatcaaca
      361 cgaattggcg atctgtgacc ttgcatttag aaagtgacaa tttgctgtaa aacaagcaat
      421 tttacagctt ctgtaatttt aaggaattcc tgtgtttcct ataattcggg actaataaag
      481 tcatggggat atcttctacg taccgcgaag tcttccatat ttttcaaatc gatacctgca
      541 aaggcggctt cataatttga acacatttgc tgcaataaat ccaagctatg ggtcttgggg
      601 aactcctctt tcttaaagac caaatacgtt tttaaatatt tttcgacagc ttgctggcag
      661 tgaaagcagg caatgtctaa aatcaaaggc tgctgctcga caatcaattt tgcggctatc
      721 aggtcgtgct cggccttttc gatccactgg tttagatgtt ttttttcggc ttcggtcata
      781 tctccacagc ctccttcatg gcataatgaa cgatatgacc agttaactcc tttttttctt
      841 ttacttcatc ttcactttcc acaatgacat cgaagggtgc tttcaaagca tatacgagcg
      901 cctttcttat cttgttttcc cagtccattt tcacccgcgg ggcaaacgtg tcatttgtaa
      961 cgatcaacaa atcatagtcg ctctgcttgt cagcctgtcc ccttgccctg gagccaaaaa
     1021 gaagcacctt tgaattcggc aaaaaggaat atacagttga tttaatggct tgaaaagtct
     1081 gctgtgcgtt catatccaga caaatataca aaatggaatc aatctttggg gaaaattaga
     1141 gaaggggagg tacgcctatt tatactcccc aaacacctcg cgcagcgtat cggcaatctc
     1201 tcccagcgta cagcggcgct ccacggcttc gacgacgacg ggcatgatgt tctcgcccga
     1261 ggcggcctta tcgttgaggt cctgtaatac ggaatccact tttgccgggt cgcgccgcct
     1321 tttcagatcg ttcagttttt cgatctgcac cgtgcggatg gagtcgtcga tccggaacac
     1381 ggggatgcta tccttttcct ccgtagggaa actgttgacg ccgacgatga tcttctcctt
     1441 tgactcgacc cgttgctggt attcgtacgc gctgcgcgcg atctcgtcct ggatgaatcc
     1501 ctgctcgatg gcggagacgc tgccgcccat ccggtcgata tgttcgatca gtttccaggc
     1561 tgcctcttcc acttcccggg taagcgcctc tacataatag ctgccggcca gggggtcggc
     1621 ggtgtcggcg gctccgctct cataggcgac gacctgttgc gtccggagcg cgatcctcgc
     1681 cgcctcttcg gtgggcaggc tcagcgcctc gtcataaccg ttggtatgca gggactgcgt
     1741 gccgccaagc actgcggcga gggtctgcac ggtcacccgg gagatattgt taaggggttg
     1801 ctgcgccgtt agcgtggagc cgcctgtctg ggtgtgaaaa cgtaacatca tggcctttgg
     1861 gtccgtggcc ccgagctctt gcataatgcg cgcccacatc cggcgggcgg ccctgaattt
     1921 ggctacttcc tcgaatagat tgttatgcgc attgaagaaa aaggagaggc gtttgccaaa
     1981 aacatcgata tccagccctt tctcgagggc ggcctgtaca taggccttcc cgttactgag
     2041 ggtaaaggcg atctcctgta cggccgtgct gcccgcctcg cggatatggt agccggagat
     2101 ggagatggtg ttccatcgcg gcaactcccg gctgcaccac tcgaagatat cggtgatgat
     2161 atgcatggag ggttttggcg gatagatata ggttccgcgg gccgcgtatt ccttcaggat
     2221 atcgttctgg atcgtcccgg tgatcttctt ccagtccgca ccctgctgtt tcgcgagggc
     2281 ggcataaaag gcaaggagga tatagccggt ggcgttgatg gtcatggaag
//
LOCUS       DATGFG010000181         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1338690, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000181 DATGFG010000000
VERSION     DATGFG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338690"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1225)
                     /locus_tag="VKQ52_03400"
     CDS             complement(2..1225)
                     /locus_tag="VKQ52_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HLZ86256.1"
                     /translation="MRRTLLMITVIAAAIMELIDTSIVNVSLSYMSGNLGATLEDTSW
                     VITAYAIANVIIIPITSFLSATLGRRNYYIGSVILFTLFSFLCGQATNIWMLVVCRFF
                     QGMGGGALLSVSAAIVYELFPKEKQNVASALFGVGIFVGPTIGPTLGGYITEHFSWPW
                     IFYINVPIGIAVALICGRLLGEPPYKPTAGRIDWTGILLLAVGVGSLQVVLERGETDD
                     WFSAGYIVVLTVIASLCLSFFLYWELKVKAPVVNLRVLKSKNLSIAALLTFVSGIGLF
                     SSVYLTPVYAQRLLGFTPTTTGLLLLPGALFAIAGLVLTARLLQRGVPPIIMITAGML
                     LFILFTWQIAGVNLDAGTGDFYLPLIFRAIGIAIVTVPLSTLAVSGLDPKDIPQGTAL
                     NNMKRQLGGSSASRW"
     gene            complement(1261..2010)
                     /locus_tag="VKQ52_03405"
     CDS             complement(1261..2010)
                     /locus_tag="VKQ52_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ86257.1"
                     /translation="MKTSNNTILITGGSAGIGFAIAKTLGANNKIIITGRDQQRLKAA
                     AAKLPNTTAILGDVSDAKDVDKLVATLNRDHKDLNIVINNAGKASGIYDLATEGIDAF
                     TRAGEEMLTNYLSVIRLNERLLPLLKKQPEAAIVNVSSIVAIAPGGRLPSYSASKAAL
                     HSYTISLRQALSKTNVAVFELYPPLVNTEFSAEIGGATNGIAPSVVADDLLKGLEQDT
                     YDIRVGRTAELYQYYLANPQEAIKAMSAPQE"
     gene            complement(2077..2661)
                     /locus_tag="VKQ52_03410"
     CDS             complement(2077..2661)
                     /locus_tag="VKQ52_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ86258.1"
                     /translation="MSKAERTRQFIIERTAPIFNTKGYAATSLSDMTDATGLTKGSIY
                     GNFTDKDEVALACFDHNHKTVREVIRQEMDKHSSYRDKLRVYVEAYSGNRKLNFVPGG
                     CPILNTAIEAADTHPALKAKAAEAITLWKERIIQIVEAGKANREFKQTTNAEQAALSI
                     IALLEGMIMIRRTIGQPGCAKPIMAAVENIIDEL"
     gene            2770..3348
                     /locus_tag="VKQ52_03415"
     CDS             2770..3348
                     /locus_tag="VKQ52_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ86259.1"
                     /translation="MFEAIDQFVAKYITLTEEESAFFHSLLRHKKLRKKQFLLQEGEV
                     CDFEGFILKGCTRTYYLDKEGVETILLFAVEEWWVSDLTSFSERKPSNMFIETIEDTE
                     LLTIDYAGKARLFERIPAFERMFRLLVQRSLGVLQQRFYASVSQTAEERYKQFLEKYP
                     LVAQRVPQHQIARYIGVSPEFLSKVRSTMFRK"
BASE COUNT          654 a          740 c         1241 g          860 t
ORIGIN      
        1 ttcaccatcg cgatgccgag gagccgccga gttggcgctt catgttgttg agtgccgttc
       61 cctgggggat atccttgggg tcgaggccgg agacggccag ggtggagagc ggcacggtga
      121 caatagcgat gccgatggcg cggaagatca ggggcaggta gaagtctccg gtaccggcgt
      181 cgaggtttac tcctgctatc tgccaggtga agaggatgaa gagcagcata ccggccgtga
      241 tcatgattat tggtgggacg ccccgttgca gcaggcgtgc ggtaaggacg aggccggcga
      301 tggcgaagag ggcgcccggc aggaggagca gtcctgtggt ggtgggggtg aaaccgagca
      361 gacgctgggc gtatacgggt gtcagataca cggagctgaa gaggccgata ccggagacga
      421 aggtcagcag ggcggcgatg ctgagatttt tgcttttgag cacgcgcagg ttgacgacag
      481 gcgcttttac tttcaattcc cagtagagga agaaggatag gcacaggctg gcgataacgg
      541 taagcacgac gatgtagccg gcgctgaacc agtcgtcggt ctcgccgcgt tcgagtacga
      601 cttgcagcga tccgaccccg actgcgagga ggaggatacc ggtccagtcg atgcggccgg
      661 ccgtgggttt gtagggtggt tcgccgagga ggcggccgca gatgagggct acggcgatgc
      721 cgatgggaac attgatataa aagatccagg gccaggagaa atgttcggtg atgtagccgc
      781 cgagggtcgg accgatggtg gggcccacga aaatgccgac gccgaagagg gcgctggcga
      841 cattttgttt ttcctttgga aagagttcat agacgatggc tgccgagacg gaaagcaggg
      901 cgccgccgcc cattccctgg aagaagcggc agactaccag catccagatg ttggtagcct
      961 ggccgcagag aaaggagaag agggtgaaca ggatcacgga accaatgtag taattccgcc
     1021 ggcccagggt tgcagagagg aaactggtga tcgggatgat gatgacattg gcgatggcgt
     1081 acgcggtgat gacccaggag gtgtcttcga gggttgcgcc gagattgccg ctcatgtagg
     1141 acagcgagac gttgacgatg gacgtgtcga tcagttccat gatggcggcc gctatgaccg
     1201 tgatcatgag gagggtcctt ctcattgttg tattttttat tcctgtggag cgctcctgtt
     1261 ttattcctgt ggagcgctca tggctttgat ggcttcctgg gggttggcga ggtagtactg
     1321 gtacagttcg gcggttctgc cgacgcggat gtcataggta tcctgttcga gaccctttag
     1381 gagatcgtcg gctacgacgg agggggcgat gccattggtt gcgccaccga tctcagcgga
     1441 gaattcggtg ttcacgagcg gcgggtagag ttcgaatacg gcaacgttcg tctttgagag
     1501 ggcctgtcgc agggagatgg tgtagctgtg cagtgcggct ttgctggcgg aatagctggg
     1561 aaggcggccg ccgggagcga tggccacgat ggaggagaca ttgacgatgg cggcttcggg
     1621 ttgttttttg agaaggggga ggagtcgttc gttcaaccgg atgacggaga ggtaattggt
     1681 cagcatctcc tcccctgccc ttgtgaaggc gtcgatgcct tcggtggcaa gatcgtagat
     1741 gccggaggct tttccggcat tgttgatgac gatgttgagg tctttgtggt cgcgattgag
     1801 ggtagccacg agtttgtcga cgtcttttgc gtcggagacg tcgccaagga tggcggtggt
     1861 gttggggagt tttgcggcgg cggctttgag acgttgctgg tcgcggccgg tgatgatgat
     1921 cttattattt gcgccgagtg tttttgcgat tgcgaatccg atgccggcgc tgccgccggt
     1981 gatgaggatg gtgttgttgg acgttttcat tttgttttgt tttttattcg ccttgcggca
     2041 ttttataccg atcggtatat tttttattaa aaaaaatcac agttcgtcga tgatgttttc
     2101 cacggcggcc atgatgggtt tggcacagcc gggctggccg atggtcctgc ggatcatgat
     2161 catgccttcg aggagggcga tgatggagag ggcggcctgt tcggcgttgg tggtttgttt
     2221 gaattccctg tttgcttttc cggcctcgac gatctggatg atcctttctt tccagagggt
     2281 gatggcttcc gcggcttttg ctttcagggc gggatgggta tcggccgctt cgatggcggt
     2341 gttgaggatg gggcagccgc cggggacgaa attgagtttg cggttgccgc tgtaggcttc
     2401 gacatagacg cgcagtttgt cgcgatagga cgagtgcttg tccatttcct ggcggatgac
     2461 ttcgcggacg gtcttgtggt tgtgatcgaa gcaggccagg gctacttcgt ccttgtcggt
     2521 gaagttgcca tagatgctgc cttttgtgag gccggtggcg tcggtcatat ccgagagcga
     2581 ggtggcggcg tagcccttcg tgttgaagat gggcgccgtt ctctcgatga tgaattgacg
     2641 ggtgcgttcg gctttgctca tgggtgtaaa attataccga ttggtattat ttgccaaatt
     2701 tattttttag aggcgccgcc cggagggcgg cgcaatagtt atattcgctt atgtttgaag
     2761 ttattgccta tgtttgaagc tattgaccag tttgtagcca aatatattac cctgacggag
     2821 gaggagtcgg ccttctttca ttcgctgttg cggcataaga agctgcggaa gaagcagttt
     2881 ttgcttcagg agggggaggt ttgtgatttc gaggggttta ttttgaaggg atgtacacgg
     2941 acctattatt tagacaagga gggggtggag acgatcctgc tttttgcggt ggaggagtgg
     3001 tgggtgagcg acctcacgag tttttcggag cggaagccgt cgaatatgtt catcgagacg
     3061 atcgaggata cggagttgct gacgatcgat tatgcgggca aggcgcggct gttcgagcgg
     3121 ataccggcgt tcgagcggat gttccggttg ttggtgcaac ggtcccttgg ggtgttgcag
     3181 cagcgattct atgcgtctgt gtcgcaaacg gcggaagagc gctacaagca gttcctggag
     3241 aagtatccgc ttgtagcgca gcgggtgccg cagcaccaga tcgcgcgata tatcggcgtg
     3301 tcgccggagt ttttgagcaa ggtgaggagt acgatgttca ggaagtaaat accacccggg
     3361 cggcttcggt gatctcggtg gtggtgatgg tgtggcccat gccggggtag atgcgttcgg
     3421 tgacggcggc gttcattttt tggagttggg ccgtggtgtc atggacgcgg cttacgggga
     3481 catggggatc ggggt
//
LOCUS       DATGFG010000182         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_997492, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000182 DATGFG010000000
VERSION     DATGFG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997492"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VKQ52_03420"
     CDS             <1..1046
                     /locus_tag="VKQ52_03420"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HLZ86260.1"
                     /translation="IIEAGFPISSPGDFSSVEEISKIIRTATVCGLSRAVQKDIETAA
                     QALKHARRPRIHTGIGTSDSHIKSKFNSNREDILQRAVQAVKWARNFVDDVEFYAEDA
                     GRTENAYLARVIEAVIAAGATVVNIPDTTGYCLPDQYGEKIAWLVNNVPNIDKAVISC
                     HCHNDLGLATANSIAGVRAGARQIECTINGLGERAGNTSLEEVVMVIKQHKDLGFYTG
                     INTKALNPMSQLVSDTMRMPVQPNKAIVGANAFSHSSGIHQDGFLKDASTYEIINPEE
                     VGAEGSRIVLTARSGRSALAFRLHKIGYSFTRNDIDTLYQRFLLVADTKKEVMEDDLH
                     QMAKAYQATAVAV"
     gene            1062..>2300
                     /gene="leuC"
                     /locus_tag="VKQ52_03425"
     CDS             1062..>2300
                     /gene="leuC"
                     /locus_tag="VKQ52_03425"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HLZ86261.1"
                     /translation="MQKTLFEKIWDRHVVKTIDGGPSVLYIDKHFIHEVTSPQAFKGL
                     EKRGIDVFRPKQIVATADHNVPTIDQHLPIKEELSRIQVQALVENTKRHHIELYGLGH
                     PYQGIVHVIGPELGVTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVMATQCLLQ
                     SKPKLMRINVEGTLNKGVTSKDIILYIIALISASGATGYFVEYAGSAIRSLSMEGRMT
                     ICNMSIEMGARGGMIAPDQTTFHYIKGREFAPKGAEWDRKLAEWKQLNSDEGALFDKE
                     ITIDAADIEPMITYGTNPGMGVSVTGQIPATADPKSLAYMGLQAGAAIKGKKVDYVFI
                     GSCTNSRIEDLRLVASFVKGRKKADNVEVWIVPGSKQVEQQAIKEGIDKVFAEAGFLL
                     RQPGCSACLAMNEDKIPAGKY"
BASE COUNT          596 a          803 c          531 g          370 t
ORIGIN      
        1 acatcatcga ggccggcttc ccgatctcga gtcccggtga cttcagctcc gtggaggaga
       61 tttcaaagat catcaggacg gctaccgttt gcggcctcag ccgcgccgtt cagaaagata
      121 tcgaaacagc cgcccaggcg ctgaaacacg cccgccggcc ccgcatccac accggcatcg
      181 gaacctccga ttcccatata aaaagcaaat tcaactccaa ccgggaagat atcctccaac
      241 gcgccgtcca ggccgtaaaa tgggcccgca actttgtcga tgacgtggaa ttctacgccg
      301 aagacgccgg ccgcaccgag aatgcctatc tcgcccgcgt gatcgaagcc gtgatagccg
      361 ccggtgctac cgtcgtcaat atccccgata ccaccggcta ctgcctcccc gaccaatatg
      421 gtgaaaagat cgcctggctc gtcaacaacg ttcccaatat cgataaggcc gtcatctcct
      481 gccactgcca caacgacctc ggcctggcca ccgccaactc catcgccggc gtccgcgcag
      541 gcgcccgcca gatcgaatgc accatcaacg gcctcggcga acgcgccggc aatacctccc
      601 tcgaagaagt agtgatggtg atcaaacaac acaaagacct cggattctat accggtatca
      661 atacaaaagc gctgaacccc atgagccaac tggtctccga tacgatgcgt atgccggtcc
      721 agcccaataa agccatcgta ggcgcaaacg ctttctcgca ctcctccggt atccaccagg
      781 acgggttcct caaggacgcc tccacctacg agatcatcaa ccccgaagaa gtaggcgccg
      841 aaggctcccg catcgtgctc accgcccgca gcggccgcag cgccctcgcc ttccggctcc
      901 acaagatcgg ctactccttc acccgtaacg atatcgacac cctgtaccaa cgcttcctcc
      961 tcgtcgccga cacaaaaaag gaagtgatgg aagacgacct gcatcaaatg gcaaaagcct
     1021 accaggccac agccgtagcc gtttaaaacc gatcgaacaa aatgcaaaag acactattcg
     1081 aaaagatctg ggaccgtcac gtcgtcaaaa ccatcgacgg cggcccctcc gtactctaca
     1141 tcgacaaaca cttcatccac gaggtaacca gcccgcaagc cttcaaaggc ctcgaaaaac
     1201 gcggcatcga cgtcttccgc ccaaaacaga tcgtcgccac cgccgatcac aatgttccta
     1261 ccatcgacca gcacctcccc ataaaagaag aactgtcccg gatccaggtt caggccctgg
     1321 tggaaaatac aaaaagacat catatcgaat tatacggcct tggtcacccc taccagggca
     1381 tcgtccatgt catcggaccc gaactgggag tcacccaacc cggcatgacc atcgtctgcg
     1441 gcgatagcca cacctccacc catggcgcct tcggcgccat cgccttcggc atcggcacca
     1501 gcgaggtgga aatggtcatg gccacccaat gcctgctgca aagcaaaccc aaactcatgc
     1561 gcatcaacgt cgagggtacc cttaataaag gcgtcacctc gaaagatatc atcctctaca
     1621 tcattgccct catctccgcc agcggcgcca ccggctattt cgtcgaatac gcaggctccg
     1681 ccatccgcag cctctccatg gaaggccgga tgaccatctg taacatgagc atcgaaatgg
     1741 gcgcccgcgg cggcatgatc gcccccgacc agacgacctt ccactatata aaaggacggg
     1801 aattcgcacc aaaaggcgcc gaatgggaca ggaaactcgc cgaatggaaa caactcaact
     1861 ccgatgaagg cgcactattc gacaaggaga tcaccatcga cgccgccgac atcgaaccca
     1921 tgatcaccta cggcaccaac ccgggcatgg gcgtctccgt aaccgggcag atccccgcca
     1981 ccgccgaccc caaatccctc gcctatatgg gactgcaggc cggcgccgcc ataaagggca
     2041 aaaaggtaga ctacgtcttc atcggcagct gcaccaactc ccgcatcgaa gacctccgcc
     2101 tcgtcgcctc cttcgtcaaa ggccggaaaa aagccgataa cgtcgaagta tggatcgtcc
     2161 ccggcagcaa acaggtcgaa cagcaagcca taaaggaagg catcgacaag gtcttcgccg
     2221 aagccggctt cctcctccgc caacccggct gctccgcctg cctcgcgatg aacgaagaca
     2281 agatccccgc gggcaaatac
//
LOCUS       DATGFG010000183         5342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_767490, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000183 DATGFG010000000
VERSION     DATGFG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767490"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VKQ52_03430"
     CDS             complement(<1..232)
                     /locus_tag="VKQ52_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86262.1"
                     /translation="MMTLKNSVGLSFEFLSNGSVKSMEVYPIRISLKAATPFSKPGTN
                     FYLRKRKKPFEFTALLGPEGNSLFKMAGDAFMA"
     gene            complement(229..2664)
                     /locus_tag="VKQ52_03435"
     CDS             complement(229..2664)
                     /locus_tag="VKQ52_03435"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HLZ86263.1"
                     /translation="MTFKIQEKNFCLNNKKVFLNSGEIHYFRIRRELWDKHLEAAREA
                     GLTTVSTYVPWAWHEAEEGVFDFDGTSCPERDLKGWLQRCQANGLTCIVKPGPFILAE
                     TRGAGLPDWFMDQYREEVRMRNSKGEIVPGEGVSLFNPIYLERVTRWYDQVMPLISKR
                     EIPAGGPIIMMQICNEIGVFSWLAHQGDYSEPVRARFISYLSVKFKSIAEMNGRWGTV
                     YPDFAHVELPPDGRLPYASKGDRSRDYEWHCFWRTYYGDYLKMLTGMARDRGVTVPFF
                     HNLPGWIYGSGYEFPVNITMYEELFGDKSEIIFGVDHIPEFLSHRNMHDDRIINDITF
                     AMQGNKPLFAAEFQCGSREYHVVTNPRELELFYKASIAHGLTGWNYYMFSQGRNPRHR
                     GYSGESFYWFSPLTPEGERTSIFPLIKRMSKIVKTSENLIVESERKAEICVLFYPPYY
                     ATELERPETGASGLRYNPSAIRRSAYFDGLLKVLQVLNVDYDMVDLCQATAEKLGRYK
                     QVWAFSTDEMNAGDQQTLVDYTRAGGRLVIFPHLPDREMSQKPCTIIRDAILVKPSGA
                     EIIDSPLIDVYDLKDIKCANPQIIYDEPSLAGAEVIARTIRGSACGFTKSLGRGSITH
                     LGTWLGFDTEGHKAVYEAILTKSGSKVRQASASNDNIAVSERFTGDQAAILFIGNYYN
                     EEQDGKVTYTHPESGEAINVPYAQNGMIWPALYGVLTPVCMEISDGIKILHSTSDILE
                     VAGNKGQLEITLYGDRDLAGEIVFEGTKVDKIKSATIYGDPVKMVRDDKRVAFGYSHR
                     HKEEMTLSVKLTR"
     gene            complement(2728..4818)
                     /locus_tag="VKQ52_03440"
     CDS             complement(2728..4818)
                     /locus_tag="VKQ52_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ86264.1"
                     /translation="MTEQELTAVHRTKPEDRIRFSQLAAYGAGGIIPIALFNIAGILV
                     GLMGNISLGLSAFWLGVILIIPRLWDALSDPIIGHLSDNTRTRWGRRRPFLLIGGILV
                     AVFFVVMWWIPKGDRVRVWFPSETGFQAFQLVYILFSLLLFFTACNIFEIPHGALGME
                     MTTDYHERTRLFSAKSFVGNLFAMSTPWLFALANMEIFKGTGNEADGMRYVSIMVAAL
                     IIPLSFWWTFQLREPGFVKAARHERTPFWKDMKRTAGNRNFVMLTLTIFTLAMGFNFV
                     QLLGSYIPIFYVFGGDKVAGAYLLGINGTIWAVTGLLAVFPLIWISPKLGKRNTLAIS
                     ILLMCLAQLSKIVCYSPSFPYLLIIPTVLLSMGMLFFFTLGSSMVGDICDEDELKTGY
                     RAEGSYYSIFWWFIKMGTAFASLVAGALIVLTAFDEIQVTKVDSLSGIIREIHSEVQS
                     WGGDQVFRGTDTELLGKTKTQITEALIEAKEYASYLEKKSGKQPAPSQESAAEYQNRQ
                     RETLLNALSSIKATAGGLEELRLRLENLGPQAQDSQVKAAVAEAIPLTFQTKFEKARM
                     YSFELMSHLESKSLESKNSAVHYQEIIKNAATLNDRLARLNAASTLHDLDDELAIIEK
                     DIPPLTKQAPYTLLMMRVVEIGLPVLLSIFSIFFLLRYSLTEKRSQEIKNLLMQRNEE
                     RAKEANQATGSAAV"
     gene            complement(4839..>5342)
                     /locus_tag="VKQ52_03445"
     CDS             complement(4839..>5342)
                     /locus_tag="VKQ52_03445"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HLZ86265.1"
                     /translation="MPTVIIDEKQYGPENVGTNDVIHAVIQRRYALDVLKYPVWGMSP
                     CSMTASNGYSEYGVKVLGVRGYKAGVVTPHVTALALYVTPAEAIANLRNLIEKYDIYG
                     QYGFYDAVDPVSGEVAHKYLVLDQGMIFLALANYLGDQCVQKHFAADPIAQKVLPMLK
                     DENFLSN"
BASE COUNT         1262 a         1391 c         1363 g         1326 t
ORIGIN      
        1 tggccataaa tgcgtctccg gccattttaa aaaggctgtt accctccggg cccagaagag
       61 cggtaaattc aaatggtttt tttcttttcc tcaggtaaaa gttcgtacct ggttttgaaa
      121 aaggagtcgc agctttgagg cttatcctga tgggataaac ttccatactc ttcacagagc
      181 cattgctcag gaattcaaag gataaaccaa cagaattctt aagagtcatc atcgtgtcag
      241 tttcacgctc aaggtcattt cttccttgtg cctgtgactg tatccgaatg cgacgcgctt
      301 gtcgtcacgg accattttta cagggtcacc ataaattgtc gcagatttga tcttatcaac
      361 cttggtaccc tcaaaaacga tctccccggc caggtctcga tctccgtaca gggtgatctc
      421 cagctggccc ttattcccgg caacctcgag aatgtccgaa gtgctgtgca ggattttaat
      481 gccatccgaa atctccatgc acaccggcgt caatacgccg taaagggccg gccatatcat
      541 cccgttctgc gcgtatggta cattgatggc ctccccgctt tcaggatgcg tataggtgac
      601 tttgccatcc tgctcttcat tgtaatagtt cccaatgaaa agaattgcag cctgatcacc
      661 cgtaaagcgc tcgctgactg ctatattatc attggatgct gatgcctgcc tgacctttga
      721 acctgatttc gtcaggatgg cttcatacac tgctttatgt ccttcagtgt cgaacccgag
      781 ccacgttccc aggtgagtga tggagccgcg gccaaggctt tttgtgaacc cgcacgcgga
      841 accgcgaatc gtcctggcga tgacttccgc accggcgagc gatggctcat cataaatgat
      901 ctgcggattg gcacacttga tgtctttcaa gtcatacaca tcgatcagcg gagaatcgat
      961 gatctctgcg ccggatggtt ttaccaggat ggcatctcgt atgatggtgc aaggcttttg
     1021 tgacatctcg cggtcaggca gatgcggaaa tataaccaac ctgccgccgg ccctggtata
     1081 atcaaccagg gtctgttgat ccccggcatt catttcatcg gtgctgaacg cccagacctg
     1141 cttgtatctg cctaattttt cagcagtggc ctggcacagg tccaccatat cataatcaac
     1201 gttgagcacc tggagcactt tcagcaagcc gtcgaaatag gccgaccggc ggatggctga
     1261 agggttgtat cgaagcccgc ttgctccggt ttcaggtctc tccagttcgg tggcataata
     1321 aggcggatag aaaaggacgc agatctctgc cttacgttct gactcaacga tcagattttc
     1381 ggaagtttta acgattttac tcatccgttt gatcaggggg aaaatgctgg tacgctcgcc
     1441 ctccggcgtc agcgggctga accagtaaaa agattccccg gaatagcccc ggtgccttgg
     1501 gtttcgaccc tgggagaaca tgtaataatt ccagccggtc agtccgtggg cgatgctggc
     1561 tttgtagaaa agttccaact cacggggatt cgtcaccacg tggtattccc gggagccgca
     1621 ctggaactcg gcggcaaaca acggcttgtt cccctgcatg gcaaaggtga tgtcattgat
     1681 gatccggtca tcgtgcatgt tgcggtgtga aaggaattcc gggatatgat cgacgccgaa
     1741 aatgatctcg ctcttgtctc cgaagagctc ttcgtacatg gtgatgttga ccgggaattc
     1801 ataaccgctt ccatagatcc agcctggaag attgtgaaaa aatggaaccg tcacccccct
     1861 gtcccgggcc atgccggtca gcatttttaa ataatcgcca taataagttc tccagaaaca
     1921 gtgccattca taatcacggc tgcggtcgcc ctttgaggca taaggaagcc tgccgtccgg
     1981 gggaagttct acgtgggcaa agtccgggta aactgttccc cagcggccgt tcatttccgc
     2041 aatacttttg aatttgactg acaggtatga aatgaaacga gcccggacag gttcagaata
     2101 gtctccctgg tgcgccagcc atgagaatac gccgatctca ttgcagatct gcatcatgat
     2161 aatcggacca ccagccggga tttcacgttt gctgatcaag ggcatgacct ggtcatacca
     2221 gcgcgttacc cgttccaggt agattgggtt gaacaggctg acgccttcgc ctggcacgat
     2281 ctcgcccttg ctgttgcgca tcctaacttc ttcccggtat tggtccatga accagtccgg
     2341 cagccccgct ccgcgggtct cggccaggat aaacggcccc ggtttgacga tgcaggtcaa
     2401 tccgttggcc tggcatcgtt gcagccagcc tttaagatcc cgttccgggc atgaggtgcc
     2461 gtcaaaatca aaaacgcctt cctctgcctc gtgccacgcc cagggaacat aagtgctgac
     2521 ggtggttaat ccggcctccc tggctgcctc caggtgcttg tcccaaagtt cccgcctgat
     2581 cctgaaataa tggatctctc ctgaattgag gaataccttc ttgttgttta aacagaaatt
     2641 cttttcctga atcttgaatg tcattctctt aattccatgt ttttgttacc gacagtcacg
     2701 gtccgatggt taaatccgtg gcttcgctta aaccgccgct gatccagtcg cctggttcgc
     2761 ttcctttgcc cgttcctcat tacgttgcat taaaagattt ttgatctcct gtgaccgttt
     2821 ttcagtcaat gagtaacgga ggagaaagaa gatggaaaaa atgctcaaca gcactggtag
     2881 tccgatctcg accacccgca tcatcaaaag agtataaggc gcctgtttgg tcagaggtgg
     2941 aatgtccttt tcgattattg caagttcatc gtccagatca tgcagggttg acgccgcgtt
     3001 cagcctggca agccggtcat ttaatgtggc agcgtttttg atgatctcct gataatgcac
     3061 ggcactgttt ttactttcca gtgatttgga ttccaggtgg gacataagct caaatgaata
     3121 catcctggct ttttcaaact tggtttgaaa tgtcagggga atggcttctg ccacggcggc
     3181 tttgacctgg gagtcctggg cctggggccc caggttctcc aaacgcaatc gtaactcttc
     3241 caatccgcct gcggtggctt ttatgctgga caaggcattc agcaaagtct cgcgctgacg
     3301 gttttggtat tcggcagccg attcctggga cggcgcgggt tgttttccag atttcttttc
     3361 gaggtagctc gcatattctt ttgcctcaat gagcgcttca gtgatttgcg ttttggtttt
     3421 tcccagcaac tctgtgtcgg tgccgcggaa aacctgatca cctccccagg actgaacctc
     3481 gctgtgaatc tccctgatta tccccgagag gctatcgacc ttggtaacct ggatttcgtc
     3541 aaacgcagtc aacacgatca gtgcgccggc gacaaggctg gcaaatgcag ttcccatttt
     3601 aatgaaccac cagaatatcg aatagtaact tccctccgcg cgataacccg tctttaattc
     3661 atcttcatcg cagatatcac ctaccatgga agatcccagg gtgaagaaaa acagcatccc
     3721 catggaaagc aggacggtgg gtatgataag caggtaggga aagcttgggc tgtagcagac
     3781 aatcttggaa agttgtgcca ggcacatgag caagatcgaa atcgcaaggg tattccgttt
     3841 gcccaatttc gggctgatcc agatcagtgg gaaaacggcc agtaatccgg tgacggccca
     3901 gatggtgccg ttgattccga gcagataagc gcccgcgact ttgtcgcccc cgaaaacata
     3961 aaagatgggg atgtatgacc cgagcagctg gacgaaattg aaacccatcg ccagggtgaa
     4021 aattgtcagg gtcagcatga caaagttccg gttgccggcg gtgcgtttca tatctttcca
     4081 gaaaggggtt ctttcatgcc tggcagcttt tacaaatccc ggctcgcgca attggaacgt
     4141 ccaccagaaa gagagcggga tgattaaagc cgcgaccatg atggacacat agcgcatgcc
     4201 atcggcttca tttcctgtac ctttgaaaat ttccatattg gcgagggcaa aaagccaggg
     4261 tgtgctcatg gcaaaaagat tgccgacaaa actcttggca ctgaaaaggc gggtgcgttc
     4321 atggtaatca gtggtcatct ccatccccag ggcaccatga gggatctcaa aaatattaca
     4381 ggctgtgaaa aataacagga gggagaaaag aatatagacc agttgaaacg cctggaagcc
     4441 tgtttctgac gggaaccaga cccgcaccct gtcacctttg gggatccacc acatgaccac
     4501 aaaaaagact gcaaccagta tgcctcctat cagcaggaag ggacgccggc gcccccaacg
     4561 ggtccttgtg ttatcggaaa ggtgaccgat aatgggatcc gaaagcgcat cccacagacg
     4621 cgggatgatc aggatgacgc ccagccagaa ggcactcagc cccaggctga tatttcccat
     4681 caaacctacg agtatgccgg cgatgttgaa aagggcgatg ggaataatgc ccccggcacc
     4741 gtaggcagcc aattgtgaaa aacgaatccg atcttccggt ttagtacggt gaaccgccgt
     4801 tagttcctgt tctgtcataa ttgaacggca aagttatatc agtttgagag gaaattttcg
     4861 tccttgagca tcgggagaac tttttgcgcg atcggatccg cggcgaaatg cttctgaaca
     4921 cactggtcgc ccaggtaatt cgcgagcgcg agaaagatca ttccctggtc aagcacgaga
     4981 tatttatggg cgacttcgcc ggaaaccggg tcgacggcgt cgtagaatcc atactggccg
     5041 tagatgtcat atttctcgat caaattgcga aggttcgcaa tggcttcggc gggtgtgaca
     5101 taaagggcca gcgcggtcac gtgcggcgtg acaaccccgg ccttgtagcc gcgaacgccg
     5161 agcactttta cgccatattc cgagtagccg ttgctggccg tcatggagca gggcgacatc
     5221 ccccaaacgg gatatttcag cacgtcaagg gcataacggc gctggatcac cgcgtggatt
     5281 acatcgttgg ttcccacgtt ttcgggtccg tattgctttt cgtcgataat gaccgtcggc
     5341 at
//
LOCUS       DATGFG010000184         4421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_767111, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000184 DATGFG010000000
VERSION     DATGFG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767111"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2375)
                     /locus_tag="VKQ52_03450"
     CDS             complement(<1..2375)
                     /locus_tag="VKQ52_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HLZ86266.1"
                     /translation="MLAKMIKEGLYSPEFERDACGIGFVANIKSNKSHQIVSDALTIL
                     ENMEHRGACGCENNTGDGAGIMIQTPHEFFFDECVRLGIHLPAYGKYGVGMLFFPRDI
                     RLKEECRDIFARSAEKLGLEILGYRKVPVNPDGIGPTALSVEPEMEQVFVACPDHIKQ
                     PDDFERKLFLLRNYASNTINNTIKKDAIGFYVASLSYKTLVYKGQLTSGQVRNYFPDL
                     SNKRVVSAFGLVHSRFATNTFPSWKLAQPFRYIAHNGEINTLQGNLNWLKTSAKSFTT
                     PYFTKEEMDMLLPIIAEGSSDSACLDNVIELLALCGRSLPHVMMMLIPEAWDGNDQMD
                     PVKKAFYEFHASIMEPWDGPASISFTDGKLIGATLDRNGLRPSRYCVTTDDRVIMASE
                     TGVLPVDPRLIKEKGRLQPGKMFVVDLEQERIISDDELKAKICSQQPYAEWLNKYKIR
                     MEELPEPRVLFTHLEHDQIFKYQKAFGYSTEDLDTIIAPMALDGKEPIGSMGTDVPLA
                     VLSDQPQHLSSYFKQLFAQVTNPPIDPIRERLVMSLASFVGGNANLLEEDPLSCHSVA
                     LKHPVLSNHELEKIRSIDTGIFQAKTLQTYFRADGKPGSLQAGIDRLCRYAVDAVEDG
                     FEVLILTDRAIDSDHAPIPSLLACSAVHHHLIRKGYRGQVGIIVEAGDVWEVHHFACL
                     IGFGATAINPYLALSGIRDMKLSGRLQTELDAEQLKKNYTKAVNEGLLKVFSKMGIST
                     LQSYQGAQIFEIIGLNRSVVDKYFTGATSRIEGMGLDEIAKEVLAKHFFAF"
     gene            complement(2588..3925)
                     /gene="ilvA"
                     /locus_tag="VKQ52_03455"
     CDS             complement(2588..3925)
                     /gene="ilvA"
                     /locus_tag="VKQ52_03455"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF006390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HLZ86267.1"
                     /translation="MKQSLANTFPSTGPSSESPIPGPGLKPGRGGWELDFHAAAKRLS
                     KVVHHTPLTYNHSLSKKYACQVYLKREDLQVVRSYKLRGAYNMMVSLPTEQLKKGVVC
                     ASAGNHAQGFAYSCRKLGVKGVVFMPIITPKQKVNQTRMFGEDYIDIILTGDTFDDCN
                     IAAQQYTREKAMTFIPPFEDLRIIEGQGTVAVEILEDLPPGETGVDFLFVPVGGGGLA
                     SGTGACFKRYSPATQVIGLEPEGAPSMVEAFKAGHPVTLDNIDRFVDGAAVKRVGELT
                     FSICHEVLDGMTTVPEGRICSTILQLYNENAIVVEPAGALSIAALAHYAELIKGKVVV
                     CIVSGSNNDIDRMQEIKERSLQYEGLKHYFLVTFAQRPGALKEFVNHVLGPNDDITRF
                     EYMQKHNKETGPALVGIELSAREDYETLIHNMKKYNINYTELNKDNTLFSYLV"
     gene            complement(3906..>4421)
                     /locus_tag="VKQ52_03460"
     CDS             complement(3906..>4421)
                     /locus_tag="VKQ52_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86268.1"
                     /translation="AVGSGYLFETDFRKEVYSDLTGERGTLMGAIQGIFAAQYEVLRK
                     KGHTPSEAFNETVEELTQSLMPLVAENGMDWMYANCSTTAQRGALDWWKKFRDATLPV
                     FTELYESVAAGKESQRSIDSNSQADYREKLDAELKELRESELWQAGKTVRSLRPENQP
                     AQKSKSEAVAS"
BASE COUNT          759 a          983 c         1558 g         1121 t
ORIGIN      
        1 ctgaaggcga agaagtgttt tgcgagtacc tctttggcga tctcgtcgag gcccatgcct
       61 tctatgcggg acgtggcgcc ggtgaagtat ttgtcgacga cgctccgatt cagtccgatg
      121 atctcgaaga tctgggcgcc ctggtaggat tggagagtcg agatgcccat cttggagaag
      181 accttgagga gtccttcgtt gacggctttg gtatagttct tcttcagctg ttcggcatcg
      241 agttctgtct ggagccggcc gctgagcttc atgtcccgga tgccggacag ggccaggtag
      301 ggattgatgg cggtggcgcc gaagccgatg aggcaggcga agtggtggac ttcccagacg
      361 tcgccggctt ccacgatgat gcccacttgt ccgcggtagc ctttgcggat gaggtggtgg
      421 tggacggcgg agcaggccag cagggagggg atgggtgcgt ggtcggagtc gatggccctg
      481 tccgtgagga tgagcacttc gaagccgtcc tccactgcgt ctacggcgta gcggcaaagg
      541 cggtcgatcc cggcctggag ggacccgggt ttaccgtcgg cgcggaaata ggtctgcagg
      601 gtctttgcct ggaagatgcc ggtgtcgatg gagcggatct tttccagttc gtgattactc
      661 agaacggggt gtttgagtgc gacgctatgg caggacaggg gatcttcttc cagcaggttg
      721 gcgttgccgc cgacgaagct ggcgagggac atgaccagtc gttcccggat ggggtcgatg
      781 ggcgggtttg tcacctgggc gaagagctgt ttgaagtagc tgctgaggtg ttggggttgg
      841 tcgctgagga cggccagcgg gacatcggtt cccatcgagc cgatgggttc ttttccgtcg
      901 agggccatgg gggcgatgat ggtatcgagg tcttcggtgg agtaaccgaa tgctttctgg
      961 tatttgaaga tctggtcgtg ttcgagatgg gtgaagagga cccggggttc gggcagttct
     1021 tccatcctga tcttgtattt gttcagccat tcggcgtagg gctgctggga gcagatcttt
     1081 gcttttagtt cgtcgtcgct gatgatgcgt tcctgttcga ggtcgacgac gaacattttg
     1141 ccgggctgga ggcggccttt ttcttttatg aggcgtgggt cgacggggag gacgccggtt
     1201 tcggaggcca tgatgacgcg gtcgtccgtc gtcacgcagt agcgggaggg gcggaggccg
     1261 ttgcggtcga gggtggcgcc gatgagttta ccgtcggtga aggagatgga ggcggggccg
     1321 tcccagggtt ccatgatgga ggcgtggaat tcgtagaagg cttttttcac cgggtccatc
     1381 tggtcgttac cgtcccaggc ttcggggatg agcatcatca tcacgtgggg gaggctccgg
     1441 ccgcagaggg ccaggagctc gatgacgttg tcgaggcagg ccgagtcgga ggaaccttcc
     1501 gcgatgatcg ggaggagcat gtccatctct tcctttgtga agtagggggt ggtaaagctt
     1561 tttgcgcttg tcttgagcca gttgaggtta ccctgcagcg tattgatctc gccgttgtgg
     1621 gcgatatagc ggaagggttg ggccagtttc caggagggga aggtattggt tgcgaaacgg
     1681 gagtgtacga ggccgaaggc ggatacgacc cgtttgttgc tgaggtcggg gaaatagttc
     1741 cggacctggc cgctggtgag ctgcccttta tagacgagtg ttttatacga aagcgaggcc
     1801 acgtagaaac cgatggcgtc cttttttatc gtgttgttga tggtattgct ggcgtagttg
     1861 cggagaagga acagtttccg ttcgaagtcg tccggttgtt ttatatggtc ggggcaggcg
     1921 acgaacacct gttccatttc cggttcgacg gagagggccg tggggccgat gccgtcgggg
     1981 ttcaccggaa ctttccggta gccgaggatc tcgaggccga gtttttcggc gctgcgggcg
     2041 aagatatcgc ggcattcttc cttgaggcgg atgtcgcggg ggaagaagag catgcctacg
     2101 ccgtatttgc cataggcggg gaggtggatg ccgaggcgga cgcattcgtc gaagaagaat
     2161 tcgtggggag tctggatcat gatgccggcg ccgtcaccgg tgttgttctc acaaccgcag
     2221 gctccgcggt gttccatgtt ctcgaggatg gtcagggcgt cggagacgat ctggtgggat
     2281 ttgttgctct tgatgttggc gacaaagcca atgccacacg catcgcgttc aaattcaggc
     2341 gagtataatc cttctttgat catttttgct agcaagatta gacgtttttc aaaatttatg
     2401 tcgttttatt agaatttctt atggcattag cagtgatcag ttggctaaaa ttacggaaaa
     2461 attcgatgtg aatggatttt tcgtaatttt tcttgtccac agattcagtg ggttttgcac
     2521 agggggatgg atgttttttt gtttgggcgc agcgtccggg agcccggtga ggggccgccg
     2581 ggaggggtta gacaagatag ctaaacaggg tgttgtcctt gttcagttcg gtatagttga
     2641 tattgtattt tttcatgtta tggatgaggg tttcgtagtc ttcgcgggcg gagagttcga
     2701 tgccgacgag ggcgggaccg gtctctttat tgtgtttctg catgtattcg aagcgggtga
     2761 tatcgtcgtt ggggcccagg acgtggttga cgaattcttt cagggcgccg gggcgttggg
     2821 cgaaggtgac gaggaaataa tgtttgagtc cttcgtattg gagggaccgt tcttttattt
     2881 cctgcatccg gtcgatatcg ttgttgctgc cgctgacgat gcacactacg actttacctt
     2941 tgatgagttc agcgtaatgg gcgagggcgg cgatgctgag ggcgccggcg ggttcgacga
     3001 cgatggcgtt ctcgttgtac agttgcagga tggtggagca gatgcggcct tcgggtacgg
     3061 tggtcattcc gtcgaggact tcgtggcaga tgctaaaggt gagttccccc acgcgtttga
     3121 cggcggcgcc gtctacgaag cgatcgatgt tgtcgagggt gacggggtgg ccggctttga
     3181 aggcttcgac catggagggg gcgccttcgg gttcgaggcc gatcacctgt gtggccgggg
     3241 aatatctttt gaagcaggcg cccgtaccgg atgcgaggcc gccgccgccg acgggcacga
     3301 agaggaagtc gacgccggtc tcgcccgggg ggaggtcttc gaggatctcg accgcgacgg
     3361 tgccctggcc ttcgatgatg cggaggtctt cgaagggggg gatgaaggtc atcgcttttt
     3421 cgcgggtgta ctgttgggcg gcgatgttgc agtcgtcgaa ggtatcgccg gtgaggatga
     3481 tgtcgatata gtcttcgccg aacatgcggg tctggttgac cttttgtttt ggggtgatga
     3541 tgggcatgaa gaccacgcct ttgacgccga gtttccggca ggagtaggcg aagccctggg
     3601 catggttgcc ggcgctggcg cagacgacac cttttttcag ctgttcggtg ggcaggctga
     3661 ccatcatatt ataggcgccg cgcaatttat aggagcggac gacctggagg tcttcgcgtt
     3721 tgaggtagac ctggcaggca tattttttcg aaagggaatg gttataggta aggggggtat
     3781 ggtgtacgac cttgctgagc cgtttggcgg ctgcatggaa atcgagttcc cagcctcccc
     3841 ggcccggctt gaggccggga ccggggatcg gagattcgga gctggggccc gtagatggaa
     3901 atgtattagc tagcgactgc ttcacttttc gatttttgag ctggttgatt ttcgggacgg
     3961 aggctgcgga ctgtctttcc ggcctgccag agttcgcttt cgcggagttc tttcagttcg
     4021 gcgtcgagtt tttcgcggta gtcggcctgg ctgttggaat cgatgctgcg ttgggattcc
     4081 ttgccggcgg ctacgctttc gtacagttcg gtaaagacgg gcagggtggc gtcgcggaat
     4141 ttcttccacc agtcgagggc gccgcgttgt gccgtggtgg agcagttggc gtacatccag
     4201 tccatgccgt tctcggcgac gaggggcatg agggattggg tgagttcttc cactgtctcg
     4261 ttaaaggctt cggagggggt atgacccttt ttgcggagca cttcgtattg tgcggcaaag
     4321 atcccctgga tggcgcccat gagggttccg cgttcgccgg taagatcgct gtatacttct
     4381 ttacggaagt cggtttcgaa gagatagccg ctaccgacgg c
//
LOCUS       DATGFG010000185         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_651084, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000185 DATGFG010000000
VERSION     DATGFG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651084"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /gene="nusA"
                     /locus_tag="VKQ52_03465"
     CDS             <1..842
                     /gene="nusA"
                     /locus_tag="VKQ52_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HLZ86269.1"
                     /translation="IGEIVSAEVYQVWKKEILLLDEEGSELILPKSEQIPQDYFKKGE
                     NIRAVVKKVELKNNSPVIILSRTSPLFLSKLLEIEVPEIFDGLIVIKKIVREPGERAK
                     VAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDVINWTNNTQLLIQRSLTPSKI
                     TNMELDSEKSHANVYLKPDQVSLAIGRKGVNIKLAQELTGYTIDVFRDNEGEPEEFDI
                     DLEEFSDEIETWVIDELKRIGCDTARSVLDLTSEELERRTDLERETIDEVRRVLKEEF
                     DKG"
     gene            904..>3212
                     /gene="infB"
                     /locus_tag="VKQ52_03470"
     CDS             904..>3212
                     /gene="infB"
                     /locus_tag="VKQ52_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HLZ86270.1"
                     /translation="MAAAKEFNIGKDTLVDFLVSKGFSKDDLKPTSKLTEDMYRSLQQ
                     EFQGDKVAKIKSDQIDLPKGSLEAKKKKEEESSTPALFKKDIPVVKKLAKEETPPPVV
                     EEAPVATPQPAPEPVQQEEPEVVKIEAPELEGPKVINKIDLSTIDSSTRPKKGGGKKP
                     GEKETTPEPVQEEAAPEPQPVAEQPRPTVAEPVAAPKEEQKEEPVYEPAAEETTPVAE
                     TRVEEPASEENEAPPVIENIKAEKLEGPKILGKIDLPVNSDTRPKQPSEEKRKRKRIP
                     IEKKDQGTSGGGGGRPGPGQHGGNQGGGNHRPPGQGRIPIQRNDRRGTGQGQGQGQGG
                     HGGHGGHGGGNRRPDPRHAPREAKEIDKKEIQEKIRQTQAKLAGAGGRGKSLKAKYRR
                     AKRDEMAEQAGSDDMMDSRLQVTEFISVSELANLMDVSYAEVISKCMSLGMMVSINQR
                     LEADVIELVAGEFGYTVEFIGIDDAAEMEEEEVEDDPEGMVPRSPVVTIMGHVDHGKT
                     SLLDYIRSANVVAGEAGGITQHIGAYQVTTATGKKITFLDTPGHEAFTAMRARGAKAA
                     DVAVIVVAADDSVMPQTKEAISHAQAAGLPMVFAINKIDRDGANPEKIKEQLANMNLL
                     VEDWGGKYQSQEISAKKGVNIDLLLEKILLEAELLDLKANPDREASGSVIEASLDKGR
                     GYVATVLVQNGTFRVGDLVVSGQYFGRVKAMFNERNKRVDDAPPSTPVLLLGLNGAPQ
                     AGEKFKVYEDESEAKEVANKRAQILREQG"
BASE COUNT          915 a          886 c          936 g          475 t
ORIGIN      
        1 ggatcggcga gatcgtaagc gctgaggtct accaggtgtg gaaaaaagag attctcctgc
       61 tggacgaaga aggcagcgaa ttgatcctgc ccaaatcgga acagatcccc caggattatt
      121 tcaaaaaagg ggaaaatatc cgggcagtag tgaaaaaggt ggagctgaag aacaactccc
      181 ccgtgatcat cctctcgagg accagcccgc tgtttttgtc gaaattgctg gagatcgaag
      241 tacccgagat cttcgacggc ctgatcgtga tcaaaaagat cgtccgcgag ccgggcgaaa
      301 gagcaaaggt cgccgtggag tcctatgacg accggataga cccggtaggg gcctgcgtag
      361 ggatgaaggg aagccgcatc catgggatcg tccgcgaact gaagaacgag aacatcgacg
      421 tcatcaactg gaccaataat acccaactgc tgatccagcg ctccctgacg ccgtccaaga
      481 ttaccaatat ggagctggac agcgaaaagt cgcatgcgaa tgtctattta aagccggacc
      541 aggtctccct ggccatcggc cgtaaagggg taaatatcaa attggcccag gaattgactg
      601 gttatacgat cgacgtattc cgtgataacg agggcgaacc cgaagaattt gacatcgacc
      661 tggaagaatt cagtgacgag atcgaaacct gggtcatcga tgaactcaaa cgcatcggtt
      721 gcgacacggc ccgtagcgtc ctcgacctca cctccgaaga actggaaaga aggacagatc
      781 ttgaaaggga aacgatcgat gaggtgagaa gggtgctgaa agaagagttt gacaaaggat
      841 aattagacta caaatagtcc tatttttgag gcaaatatgg cagaaacaac gacattacgc
      901 ttaatggctg ccgccaagga gttcaacatc gggaaagata ccctggtgga cttcctggtg
      961 agcaaaggat tcagtaaaga cgatctgaag cctacgtcca aactgacgga ggatatgtat
     1021 cgctctttgc aacaggaatt ccagggagac aaggtcgcca agataaagag cgatcagatc
     1081 gacctgccaa aagggagcct tgaagcaaaa aagaaaaaag aagaagagag cagcacaccg
     1141 gcgcttttca agaaagacat cccggtcgta aaaaaactgg caaaggaaga gactccaccc
     1201 ccggtggtgg aggaagctcc tgtcgcgacg ccgcagccgg cgccggaacc cgtccagcag
     1261 gaagagcccg aggtcgtcaa gatcgaggct ccggaactgg aaggcccgaa ggtcatcaat
     1321 aagatcgatc tctcgactat cgattcgtcc acccggccaa agaagggtgg gggaaagaag
     1381 cccggcgaaa aggaaacgac gccggaaccc gtccaggaag aggcggcgcc cgaaccgcag
     1441 ccggtggccg aacagcccag accgaccgtc gcggaacccg tagccgctcc aaaagaagaa
     1501 caaaaggaag agccggtata cgaacccgct gcggaagaaa ctacacccgt agccgaaacc
     1561 cgggtggaag aacccgcctc cgaagagaat gaggcccccc ccgtgatcga gaatataaag
     1621 gcggaaaaac tggaagggcc aaagatcctc ggaaagatcg acctgcccgt caacagcgat
     1681 acccggccaa aacagccttc cgaagaaaaa cggaaacgga aacgcattcc catcgagaaa
     1741 aaagaccagg gcaccagcgg cggcggcggt ggcagacccg gccccggtca gcacggcggc
     1801 aaccagggcg gcggcaatca tcgccccccg ggccagggcc gcatcccgat ccaacgcaac
     1861 gaccgtcgcg gtacgggtca gggccaggga caaggccagg gtggtcacgg cggccacggt
     1921 ggtcatggcg gcggcaatcg ccggccggat ccccgtcatg caccccggga agcaaaagag
     1981 atcgataaaa aagagatcca ggagaagatc cgccagacgc aggcaaaact cgccggcgcc
     2041 ggcggtcgcg ggaaaagcct gaaagcaaaa taccgccgcg ccaaacggga cgaaatggcc
     2101 gaacaggcag gcagcgacga catgatggac agccggttac aggtgaccga attcatcagc
     2161 gtcagcgagc tcgccaacct gatggacgtc agctatgcag aagttatcag caagtgtatg
     2221 agcctgggta tgatggtgtc catcaaccaa cgcctggaag ccgacgtcat cgaactggtg
     2281 gccggtgaat tcggttatac cgtcgaattc atcgggatcg acgacgccgc cgaaatggaa
     2341 gaggaagaag tagaggatga tccggagggc atggttcccc gctcgcccgt cgtaaccatc
     2401 atgggtcacg tcgaccacgg taagacctcc ctgctcgatt atatccgcag cgccaatgta
     2461 gtggccggtg aggccggcgg catcacccag catatcggcg cctaccaggt gaccacggca
     2521 acgggaaaaa agatcacctt cctcgatacc ccgggtcacg aagcgttcac cgccatgcgc
     2581 gcccgtggtg caaaagcggc cgacgtcgcc gtcatcgtgg tcgcagcgga tgactccgtg
     2641 atgccccaga ccaaggaagc catctcgcac gcccaggcgg cagggttgcc catggtcttt
     2701 gccatcaaca agatcgatag ggatggagcc aaccccgaaa agataaagga acaactggct
     2761 aacatgaacc tcctggtcga agactggggc ggtaaatacc agtcccagga gatctccgcc
     2821 aagaaagggg tcaatataga cctcctgctg gaaaagatcc tcctcgaagc cgaactgctc
     2881 gacctgaagg caaaccccga tcgcgaagcc tcgggcagcg ttatcgaagc atcgctggat
     2941 aaagggcgcg gctatgtggc gacggtactc gtacagaacg ggaccttccg cgtcggcgac
     3001 ctggtggtct ccggccagta cttcggccgc gtaaaagcca tgttcaacga aaggaacaaa
     3061 cgcgtcgacg acgcaccccc ctccacaccc gtactgctcc tcggcctcaa cggagccccg
     3121 caggcaggtg agaagttcaa ggtgtacgaa gacgaaagcg aagcaaagga agttgcgaac
     3181 aaacgcgccc agatcctccg cgaacaaggc at
//
LOCUS       DATGFG010000186         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1468111, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000186 DATGFG010000000
VERSION     DATGFG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468111"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..>2544
                     /locus_tag="VKQ52_03475"
     CDS             68..>2544
                     /locus_tag="VKQ52_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86271.1"
                     /translation="MYTVYDGRDLPVMTQDSNLRVQGKWLCTLYDAVDRAVMTAFITY
                     SGTRGQLQAVVTGQTGSYSTGSIGVSGQSAAVASANLSLYGPQTGDFYASNQIDLDTS
                     FSAPGGGSEFSAQIATGGAIMQSSGTVQISDNPLPSGTTPDILTVTYYDNYIWVNGNA
                     LGSAFVMPISAEFSTNYNASPDYAQPPTAYYNATGLVTGTMTRVLGTTNQYLYTVSFF
                     DDHHRMIQIRQVNYSGQIDQLTTQYSFTGKPLRVFLEHQKAGTTLDKHTVMTRIAYDA
                     EQRESAVYKNIDGSSVDQLIDSMVYDAMGQLRVKCVGTNPLTGAPLDSLVYDHNIRGW
                     MTGINRNFLDSVSPHYFGMELGYDKTASIAPGTVYANPTFNGNIAGLVWKSAGDGVDR
                     KYDFSYDAANRLLGAAYTDNGGGAWNANKMDYTVNGLTYDANGNILTMNQHGFRVGNP
                     TGAIDSLTYQYQLSNASNKLSQVMDAANDTASLIGDFHYKGTKGSYDYSYDGNGNLVQ
                     DNNKAIDYIVYNYLNLPQQVHMTGKGNIFYTYDAEGNKLQKQTIDSLSALATTTTYMT
                     GGFQYQRRAPIANPSGGTDTLQFLGHEEGRARWAFHRHLAGDTAYGWEYDFMERDHLG
                     NVRVLLTQEKDTAEYMATMEPQYRATEDALFYNIDSTSYAANLVPHGGFPAEPNGPSP
                     NDSVAMVDGAGHKIGPAIILKVMSGDSVSIGCYAYYASGGVVNSPNSSFTNVLNSLAS
                     GLFTLTAGTHGTTSAMTNGSTGPVYASVNSFLSSKDTNTTVAPKAYLNWLLLDNQLNY
                     VGGSSQSGAIPVGQANALNTLGKGIRL"
BASE COUNT          657 a          649 c          683 g          555 t
ORIGIN      
        1 atgttaccgt tacgagtatg acggccggcg gcgtgtgatt gacaagaaaa taccaggggc
       61 cggagtcgtg tacacggtat atgatggccg ggatctgccc gtcatgaccc aggacagcaa
      121 tctgagggta cagggcaagt ggctttgtac gctgtatgac gcggtggatc gagctgtgat
      181 gacagcgttc atcacctata gcgggacgcg gggccagctg caggctgttg tgacgggtca
      241 aacgggtagc tacagcaccg gttccattgg cgtgagcggc caatcggccg ccgtagcatc
      301 ggcgaacctg agtctttacg ggccacagac gggcgacttt tatgccagta accagatcga
      361 cctggacacg agcttttctg ctcctggcgg aggaagcgag ttcagcgccc agatagctac
      421 cggcggagcg ataatgcaat ccagcggcac agtacagatc tcggataatc ccctaccatc
      481 cggtactaca ccggatatcc taaccgtcac ctattacgac aattacattt gggtgaacgg
      541 caacgcgctc ggatcagcct tcgtaatgcc catatcagcg gaattcagca ccaactacaa
      601 tgcttccccc gactatgctc aacctccaac ggcttattac aatgctaccg gtctcgtaac
      661 agggaccatg acgcgagtgt tgggaacgac aaatcagtac ctatatactg tttctttttt
      721 tgatgaccac caccggatga tccagatccg gcaagtgaac tactcggggc agattgacca
      781 actgacgacg caatatagct ttacgggcaa gccattgcgc gttttccttg agcaccaaaa
      841 ggcgggtacc accctcgata agcacacggt gatgacgcgg atagcatatg atgccgaaca
      901 gcgggagagt gccgtatata aaaatatcga cgggtcttcg gttgatcagt tgatcgatag
      961 catggtctac gatgcaatgg gccaactgcg ggtaaaatgc gtgggtacta accccctgac
     1021 aggcgcaccc cttgacagcc ttgtctacga ccacaacatc cgcggttgga tgacaggaat
     1081 caataggaat tttcttgata gcgtatcgcc gcattatttc gggatggagc tgggatatga
     1141 taagacagct tccatcgcac ctggtacggt ctatgctaat cccacgttca acggcaatat
     1201 tgcgggtttg gtatggaaga gcgcgggaga cggcgttgac cgaaagtatg atttctccta
     1261 tgacgcggcc aacaggctcc tgggtgctgc ctataccgac aacggcggcg gagcctggaa
     1321 tgctaataaa atggactaca cagtgaatgg tctcacttat gacgcgaacg gcaatatcct
     1381 gacgatgaac cagcatggct tccgcgttgg aaaccccact ggcgccatcg attcgcttac
     1441 ctaccagtac caattgagca atgccagcaa taagctctca caagtcatgg acgccgccaa
     1501 tgacacggca agtctgatag gggacttcca ctataagggg acgaagggat catatgacta
     1561 tagttacgat ggaaatggca accttgttca agacaacaac aaagctatcg attatattgt
     1621 ctacaattac cttaacctgc cacagcaggt tcacatgacg ggcaagggaa atatatttta
     1681 tacctatgat gcggaaggga ataaacttca aaagcagacc atcgacagct tgtccgctct
     1741 cgcgacaacg acgacctaca tgaccggcgg gttccagtat caacgacggg cacctatcgc
     1801 taatccttcg gggggcacgg atactctgca atttctcggt cacgaagaag gtagggcccg
     1861 atgggccttc caccggcatc tggcaggcga cacggcctat gggtgggaat acgatttcat
     1921 ggagcgcgat cacctgggaa atgttcgggt gctgttaacc caggaaaagg acacggcgga
     1981 gtatatggcc actatggagc cgcaataccg ggccactgag gacgctttgt tttacaatat
     2041 tgattccacc agctacgcag cgaacctcgt gccgcatggc ggatttccgg cagaacctaa
     2101 cggcccaagc ccaaatgatt ccgtagcgat ggtcgacggt gctggacaca agatcgggcc
     2161 ggcgataatt ctaaaagtga tgagtggaga ttctgtttcc attggttgct atgcgtatta
     2221 tgcatcgggg ggagtggtta actcacccaa cagctcgttt acgaacgtat tgaattccct
     2281 cgcaagcggg ctttttaccc tcactgcggg cacgcacggg acgacatcag ctatgacgaa
     2341 cggatcgacg ggtcctgttt atgccagcgt caatagtttc ctatcgtcca aagacactaa
     2401 cacaactgtt gcaccgaagg catatttaaa ctggctttta cttgataatc agctaaatta
     2461 tgtcggaggg agtagtcaaa gcggtgccat cccagtcgga caggcgaatg cgcttaatac
     2521 gttggggaag ggcatcaggc taaa
//
LOCUS       DATGFG010000187         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_424978, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000187 DATGFG010000000
VERSION     DATGFG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424978"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VKQ52_03480"
     CDS             <1..613
                     /locus_tag="VKQ52_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLZ86272.1"
                     /translation="SWHSATGATDNAAGCTVMMEVVRILKTLGIQPRRTIRIALWSGE
                     EEGLLGSRGYVKKTFADPATMQLQPAHSTFDAYFNIDNGTGKVRGIYLQGNEACSAIF
                     TQWLQPFHDLGATTVNVSNTGGTDHQSFDAVGLPGFQFIQDEIEYSTRTHHTNMDSYA
                     HLREDDLKQIATIVAAFVYNTAQRDEKLPRKELPKPRPAGRGF"
     gene            817..>2665
                     /gene="mutS"
                     /locus_tag="VKQ52_03485"
     CDS             817..>2665
                     /gene="mutS"
                     /locus_tag="VKQ52_03485"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLZ86273.1"
                     /translation="MQQHRAIKQRYPDAILLFRVGDFYETFGEDAIVASRILGITLTK
                     RNNGAAASAELAGFPHHSLDTYLHKLVKAGHRVAVCDQLEDPKSVKGIVKRGVTEMVT
                     PGVATHDKLLEGSSNNFLAGIFFPEEWPAGNATLGLSFLDISTGEFFVAEGNTEYADK
                     LLQTLRPAEVIFQRSFQKHFKEIFGSRFYTYTMDSWLFESGFATETLLKHFQTHSLKG
                     FGVEEMKAGIISAGAVIHYLKDTEHPHLQHLSTIQRIDREDYLWMDRFTIRNLELLGP
                     ASGEQGQSLLKVLDNTVSPMGARLLKRWLLMPLKDARRIHERLDTVEFFIKDPDLRTA
                     LAQHIHQCGDIERLVSKIPIRKIGPREIMQLARGLEQIAAIKEAAAAFPAGWLHDTAQ
                     QLDPCPPVHERIMATIADNPPAVAAKGDTIRTGVSEELDRLRSIATDGKEYLVRLQQK
                     EAERSGISSLKIGFNNVFGYYLEVTNTHKNRVPADWMRKQTLANAERYITPELKEYEE
                     TITGAEEKILALEGELYEHLLSFLQASIAPMQANGHRLAEMDCLLCFASNALQYNYKK
                     PDIHEGRELDLKGSRHPVIERQLPVGEAYIPNDLLLDPATQQIIILTGPN"
BASE COUNT          656 a          926 c          628 g          455 t
ORIGIN      
        1 ctcctggcac agcgccaccg gcgccacgga taacgccgcc ggctgtaccg tgatgatgga
       61 ggtggtccgt atcctgaaaa ccctgggcat ccaaccccgc cgcaccattc gcatcgccct
      121 ctggagcggt gaagaagaag gcctcctcgg cagccgaggt tatgtgaaaa agacctttgc
      181 cgatccggca accatgcaac tgcagcccgc acactcgaca ttcgacgcct acttcaatat
      241 cgataacggc acgggtaagg tccgcggcat ctacctccag ggcaacgaag cctgcagcgc
      301 catcttcacc caatggctgc aacccttcca cgacctcggc gcgactacgg tcaacgtcag
      361 caataccggc ggcaccgacc accagtcctt cgacgccgta ggcctccccg gcttccagtt
      421 catccaggac gagatcgaat atagtacacg tacccaccat accaatatgg actcctacgc
      481 tcatctgcgc gaagacgatc tcaaacagat cgccaccatc gtcgcagcct tcgtctacaa
      541 taccgcccag cgtgatgaga aactgccgcg aaaagaactg ccaaaaccac gccccgccgg
      601 ccggggcttc tgaccgccga tcccggccga tgcccgatca ctgctgatca ccgccgatcc
      661 cggccgatgc ccgatcactg ctgatcaccg ccgatcgcca atcaacaact tatccccatc
      721 caaaagcccc ggcaaacgaa ttgtcggggc tttcttacat ttgctgcctt acattcgctc
      781 cctcccatgg caaaaagcag cgccgatact ccccttatgc aacaacaccg ggctatcaaa
      841 caacgatacc ccgatgccat cctgttgttc cgcgtcggcg acttctatga gacctttggc
      901 gaagacgcca tcgtcgcctc caggatactc ggcatcaccc tcacaaaacg gaacaacggc
      961 gccgccgcct ccgcggaact ggcaggcttt ccacatcact ccctcgatac ctacctgcac
     1021 aaactcgtaa aagcgggaca ccgcgtcgcc gtctgcgatc agctggaaga ccccaaatcc
     1081 gtaaaaggca tcgtaaaaag aggcgtcacc gagatggtca ccccgggagt cgccacccac
     1141 gataaactcc tcgaaggcag cagcaataat tttctcgcag gcatcttctt cccagaagaa
     1201 tggccggccg gcaacgctac cctcggcctc tccttcctgg acatctccac gggagaattc
     1261 ttcgtggcgg aaggcaacac cgaatacgcc gacaaactcc tgcagacact tcggcccgcc
     1321 gaagtcatct tccagcgaag ctttcaaaaa catttcaagg aaatattcgg cagccggttc
     1381 tacacctaca ccatggatag ctggctcttc gaatcaggct tcgccacgga gaccctgtta
     1441 aaacattttc aaacccattc cctcaaaggg ttcggcgtcg aagaaatgaa ggcgggcatc
     1501 atctccgccg gcgccgtcat ccactatctg aaggacacgg aacacccaca ccttcaacat
     1561 ctttccacca tccagcgcat cgatcgggag gattatctct ggatggatag atttaccatc
     1621 cgtaatctcg aactgctggg gcccgcaagc ggcgaacagg gacaaagcct gttgaaagtg
     1681 ctcgacaata cggtatcccc catgggcgca cgcctgctga aacgctggct cctgatgccg
     1741 ctgaaagatg cccggcgcat ccacgaacgc ctggatacgg tagaattctt tataaaggac
     1801 cccgacctcc gcaccgccct cgcccagcat atccaccagt gcggggatat cgaacggctc
     1861 gtaagcaaga tcccgatccg caagatcggc ccccgcgaga tcatgcaact ggcccgcggc
     1921 ctcgaacaga tcgcggcgat aaaagaagcg gccgccgcct tccccgccgg ctggctccac
     1981 gacaccgccc aacaactcga cccctgcccg cccgtgcacg agcgcatcat ggccaccatc
     2041 gccgacaatc cacccgccgt cgccgcaaaa ggcgatacca tccgcacagg agtaagcgaa
     2101 gaactcgacc ggctccgcag tatcgccacc gatggcaagg aataccttgt ccggctccag
     2161 cagaaggagg ccgaacgctc gggcatctcc tcgctgaaga tcggtttcaa caacgtgttc
     2221 ggctattacc tggaagtcac caatacccat aagaaccgcg tgccggcgga ctggatgcgc
     2281 aaacagaccc tggccaacgc cgaacgctat atcacgccgg agctaaagga atacgaggag
     2341 acgatcaccg gcgccgaaga aaagatcctg gcgctcgaag gagaactcta cgaacatttg
     2401 ctgtccttcc tccaggcatc catcgcaccg atgcaggcca acggccaccg gctcgcagaa
     2461 atggattgcc tgctctgttt tgcctccaac gcccttcagt acaattataa gaagccggac
     2521 atccacgaag gcagggagct cgatctcaaa ggcagccgcc accccgtcat cgaaagacaa
     2581 ctgcccgtcg gcgaagccta tatcccgaac gacctgctcc tcgatcccgc cacccaacag
     2641 atcattattc tcaccggccc caata
//
LOCUS       DATGFG010000188         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_541740, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000188 DATGFG010000000
VERSION     DATGFG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541740"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..257
                     /locus_tag="VKQ52_03490"
     CDS             141..257
                     /locus_tag="VKQ52_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HLZ86274.1"
                     /translation="MGRGDKKTKKGKIFKGSFGKSRPAKPAKKSVPAAKEGK"
     gene            complement(364..1008)
                     /gene="pdxH"
                     /locus_tag="VKQ52_03495"
     CDS             complement(364..1008)
                     /gene="pdxH"
                     /locus_tag="VKQ52_03495"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HLZ86275.1"
                     /translation="MSTTIADIRKEYMLQTLSEKDILQDPFGQFSKWWQEAINAGIDE
                     VNAMTVATASADGLPDARILLLKGFDEKGFVFYTNYNSTKGQQLLENPRATLVFFWKE
                     LERQVRISGLVSLAPGKMSDDYFNSRPESSRIGAWASPQSEPIESRDWLEEKENGVRQ
                     EWTGKTIPRPAHWGGYVVKPVKMEFWQGRPSRLHDRIQYSLETTGTWKIERLAP"
     gene            complement(1071..>2110)
                     /locus_tag="VKQ52_03500"
     CDS             complement(1071..>2110)
                     /locus_tag="VKQ52_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86276.1"
                     /translation="ALILGFTIHFFWNSKKSFRINEPAQATGISENDNWKLKYYNDMD
                     MQERSQQQLRERLSQAQETEQILTIELDENRKELEDVRHELDIVRAELEEVELKLAPA
                     NLPAADEVTEGAPIEETQPDYAHDYLYQLKAAQEKLVEHNNSIYRLLEQTRLVEDSER
                     RNQDLMRLNDELNDQIRNTAQLLVEKETEISNLRHQQKLSEEMTQRLDKVYEEYNTLQ
                     DKLQKLQAYLTQPYNRGTDYDGLKEAYFKLGKEHDEMKLKYISIREENQRLTRILSDT
                     EEKLKEANFQRLQYQKRSAFLEELNHDLQEIAEHNKKIESQLRRVTDMENLLAKITGS
                     ASGHDISQGME"
BASE COUNT          388 a          503 c          608 g          611 t
ORIGIN      
        1 gtctgtcctt cggacgagcc gcctccggcg gctttgaata agtgctactc cggccgattt
       61 gatcagtggc caaagcaaag ctccgatgcc cctggtaccg gagcttttgt acctttagga
      121 ataaattaaa aaacacagat atgggaagag gtgataagaa aaccaagaaa ggaaagatat
      181 tcaagggttc ttttggcaaa agccgcccgg cgaagcctgc gaagaaatca gttcctgccg
      241 ccaaagaagg caagtaagat aaaagcgatc gccggggtca gttgtaccgg cgatctttgt
      301 ttgtatgggg atgttccctc attcagggag gtcagtcttt tattcagagg gccaattttt
      361 tattcagggg gccagtcttt cgatcttcca ggttccggta gtttccaggc tatattggat
      421 cctgtcatgt aaacggctgg gtcgtccctg ccagaattcc atcttcaccg gttttactac
      481 atagccgccc cagtgagcgg gtcgcggtat tgtcttaccg gtccattcct gccggacccc
      541 gttctccttt tcttccagcc agtccctgct ttcgatcggt tcgctttgcg gcgaggccca
      601 ggcgccgatg cggctgcttt ccggccggct gttgaaatag tcatcgctca ttttcccggg
      661 cgccagtgat acgagcccgc tgatgcgaac ctggcgttcc agttccttcc agaagaagac
      721 cagggtagcc cttggatttt cgaggagttg ctgtccctta gtgctattgt agttggtgta
      781 gaaaacgaac cctttttcat cgaacccctt tagcaggagg atgcgggcgt cgggcagccc
      841 gtcggcggag gcggtggcga cggtcatggc gttgacctca tcgataccgg cgttaatggc
      901 ttcctgccac catttactga attgcccgaa gggatcttgc aggatatcct tttccgaaag
      961 cgtttgcagc atgtattcct tacgaatatc ggcgatggtt gttgacacgg atggaaatgg
     1021 tttttagacc acgaaattac gcgaattacc gtaattgggt ttgccgggga tcattccatg
     1081 ccctgagaaa tatcatggcc ggaggcggag ccggtgatct ttgcgaggag gttctccata
     1141 tcggtgacgc ggcggagctg gctttctatc ttcttattgt gctcggcgat ctcctgcaga
     1201 tcgtggttga gctcttcgag gaaggccgac cgcttctggt actggaggcg ttggaagttg
     1261 gcctccttca atttctcttc ggtgtcgctg aggatgcggg tgaggcgctg gttctcttcg
     1321 cggatggaga tatatttcag tttcatctcg tcgtgttcct taccgagttt gaaataggct
     1381 tccttcagtc catcgtagtc ggtgccgcgg ttatagggct gggtgagata ggcttgtaat
     1441 ttctgcagtt tatcctggag ggtattgtat tcttcgtata ctttgtcgag gcgttgggtc
     1501 atttcctcgg acagtttttg ctgatggcgg agattgctga tctctgtttc tttttcgacg
     1561 agcaattggg cggtgttacg gatctggtcg ttgagctcgt cgttgagccg catcaggtcc
     1621 tggttgcgcc gttcggaatc ttctaccagc cgggtctgtt cgaggaggcg gtaaatgctg
     1681 ttattgtgtt ccaccagctt ttcctgggcg gcttttaact ggtagagata gtcgtgtgcg
     1741 tagtcgggtt gtgtctcttc gatgggagcg ccttccgtta cttcgtcggc ggcggggagg
     1801 ttggcgggtg cgagtttcag ttctacttct tccaattccg cgcgaacgat atccagttca
     1861 tgccggacgt cttcgagttc tttccggttc tcgtcgagtt cgatggtgag gatctgttcg
     1921 gtctcctgtg cctgggagag gcgttcccgc agttgttgct gggacctctc ctgcatgtcc
     1981 atatcgttgt agtatttcaa cttccagtta tcgttctcgc tgatgccggt ggcctgggca
     2041 ggctcattta tgcgaaagct ttttttggaa ttccagaaga agtgaatggt aaacccgaga
     2101 attagcgcac
//
LOCUS       DATGFG010000189         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_424847, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000189 DATGFG010000000
VERSION     DATGFG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424847"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="VKQ52_03505"
     CDS             complement(<1..1243)
                     /locus_tag="VKQ52_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HLZ86277.1"
                     /translation="MKAILLAFICFSLGVPLRVGLFVTSPRPHSSASGLSTPITDASR
                     PQDFAVKAFHLDMRIQVMTLPALKAFAQKLHAGGINTLIMEYEGTYPFEKHPLIANRY
                     AYTRAEITSFIAFCGQLGIDVIPLQQSFGHVEYILRHERYGPLREDEKALSQVCPLRT
                     TEDSLLFTELYTDLSQTHPSPYIHIGCDETYLLGHCDRCRRKVAQEGVSKLYIDYVSM
                     LCNIVLRLGKKPVLWADMVLKYPEAIRSLPAGTILVDWNYGWSMDRFGDHQKLLASGY
                     TIWGAPAIRSSPDNFYGTKWEKHFGNIRDFVPAAWQMGYQGMVLTSWSTSGAYSYVYE
                     SENELTNLYAIRHVYPITGFNILLAAYIESLRSAAPLDIPHFIADYCRRQYGFTPDQA
                     AAFRQALTDSSAAAGAPALRAL"
     gene            complement(1246..2004)
                     /locus_tag="VKQ52_03510"
     CDS             complement(1246..2004)
                     /locus_tag="VKQ52_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HLZ86278.1"
                     /translation="MQSDSLRDNIRVRIFDHRSQLGEAAAQTVADTIRQLQKGQQAVN
                     MIFAAAPSQNEFLAALCRQPLDWSRINAFHMDEYLGPDPASPQSFGHYLRAHLFDKVS
                     AGSVHFINGSTPDPAAECRRYAALLDRFPTDIVCMGIGENNHLAFNDPPVADFRDPEK
                     VKVVELDADCRQQQVNDGCFPTLAAVPKQALTLTIPALMQGRYIFAMVPGERKAAAIA
                     NTFNNPVTERYPSTIVKQHAAVQLFLDRDSSKLI"
     gene            complement(1991..>2910)
                     /locus_tag="VKQ52_03515"
     CDS             complement(1991..>2910)
                     /locus_tag="VKQ52_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ86279.1"
                     /translation="TTLSAGREDLLNTLESYDRVCGQPGVGARFIGMHLEGPYFALNQ
                     RGAQDPRYIRNPDPAEYRELTTRFPSIRRWSAAPELPGALDLADWLREKGILAAWAHT
                     DALYEEVLKGFERGYTHATHLYSAMLGVTRRGATRYAGAIEAAFLIDEMTVEIIADGV
                     HLPPPLLQLICKIKGADKIALITDAMGGAGMPPGESILGGKKNGLPVIIEDGVAKLPD
                     RSAFAGSVATADRLVRNMIQLAGQPLCDAVRMMTATPAAIMGVGDRKGSLIAGRDADI
                     VIFDEEINIRCTIVGGNIVYQKEQLHAKR"
BASE COUNT          547 a          795 c          978 g          590 t
ORIGIN      
        1 ggagcgctcg cagggcaggg gccccggcgg cggcactgct gtcggtgagc gcctgccgga
       61 aggccgcggc ctggtcaggg gtgaacccgt attgacgccg gcagtagtcc gcgatgaaat
      121 gggggatgtc caggggcgcc gcagagcgga ggctttcgat ataggcggcg agcaggatat
      181 taaaaccggt gataggatat acatggcgga tggcatagag attggtcagt tcgttctccg
      241 attcatagac gtaggagtag gcgcctgaag tggaccagga ggtgagcacc atcccctggt
      301 agcccatctg ccaggcggcg gggacgaagt cgcggatatt gccgaagtgt ttttcccatt
      361 ttgtgccata gaaattatcg ggactgctgc ggatggcggg cgcaccccag atggtatagc
      421 cgctggccag gagtttttga tgatcgccaa accgatccat gctccatcca tagttccaat
      481 ccaccaggat cgttcccgcc ggcagcgacc ggatggcttc gggatatttc agcaccatgt
      541 ctgcccagag gaccggtttc ttgcccagcc gcagcacgat gttacagagc atgctgacat
      601 aatcgatata cagtttggat accccttcct gcgccacctt ccggcggcag cgatcgcagt
      661 ggccgagaag gtaggtctcg tcgcagccga tgtggatgta gggggagggg tgagtctggg
      721 acagatcggt gtaaagttcg gtgaagagca gactgtcttc ggtggtccgc agaggacaga
      781 cctgagagag ggctttttcg tcttcccgca acggaccata gcgttcgtgg cgaaggatat
      841 attccacgtg gccaaagctt tgttgcaggg ggatcacgtc gatacccagc tgaccgcaga
      901 aggcgatgaa ggaggtgatc tccgcccttg tataggcata gcggttggcg atgagcgggt
      961 gtttttcgaa ggggtaggtc ccctcgtatt ccatgatgag ggtgttgatg ccgccggcat
     1021 ggagcttttg ggcaaaggct ttcagcgcgg gcagggtcat tacctggatg cgcatgtcga
     1081 ggtggaaggc cttgacggcg aagtcctgcg ggcggctcgc gtcggtgatc ggagtggaaa
     1141 gcccggaggc cgaggaatga ggccgaggac ttgtaacgaa gagcccgacc cggaggggga
     1201 cgcccaacga aaaacagata aaggccaaca ggatcgcttt catgcttaaa tgagtttgct
     1261 gctgtcgcgg tcgaggaaga gctgcaccgc ggcatgctgt tttacaatcg tcgaaggata
     1321 tctttcggtc acggggttgt tgaaggtgtt ggcgatcgcc gcggccttgc gttcgccggg
     1381 caccattgca aagatataac gcccctgcat cagcgcgggg atggtgaggg tcagcgcctg
     1441 ttttggcacc gccgccagcg tgggaaagca gccgtcgttc acctgttgtt gccggcagtc
     1501 ggcatccagc tccacgacct tcaccttttc cgggtcccgg aaatccgcca cgggcgggtc
     1561 gttaaacgcc agatggttgt tctccccgat ccccatacag acgatatcgg tggggaaccg
     1621 gtccagcaac gcggcatagc gacggcattc ggcggccgga tcgggggtgg agccgtttat
     1681 gaaatggacc gatcccgcgg ataccttgtc gaagagatgc gcccttaaat agtggccgaa
     1741 agactgggga gaggccgggt cgggcccgag gtattcgtcc atgtgaaagg cgttgatccg
     1801 gctccagtcg aggggttgcc ggcagagagc cgcgaggaat tcgttttggg agggagccgc
     1861 cgcgaagatc atgttcaccg cctgctgccc tttttgtaac tgccggatgg tgtcggcaac
     1921 ggtctgggcg gcggcctcgc cgagctggct gcgatggtcg aagatgcgga cgcggatatt
     1981 gtccctgagg ctatcgcttt gcatggagtt gttctttttg gtagacgata ttgccgccga
     2041 cgatggtgca gcggatgttt atctcctcgt cgaagataac gatatcggca tccctcccgg
     2101 cgatcaggga gcccttgcga tcgccgacgc ccataatggc cgcgggggta gccgtcatca
     2161 tccggacggc atcgcatagg ggctgtcccg ccaattggat catgttccgc accagccggt
     2221 cggcggtggc cacgcttccg gcaaaggcac ttcgatcggg cagcttcgcg accccgtctt
     2281 cgatgatcac aggcagaccg ttcttttttc cgccgaggat gctctcgccc ggcggcatgc
     2341 cggcgccgcc catggcatcg gtgatcaggg cgatcttgtc ggcccctttt atcttacaga
     2401 tcaattgcag gagcggcggg ggaagatgga cgccgtccgc gatgatctcc acggtcatct
     2461 cgtcgatgag aaaggcggct tcgatggccc cggcatagcg ggtggcgccc cgcctggtga
     2521 cgcccagcat ggccgaatac agatgggtgg catgggtata gccacgttcg aagcctttca
     2581 gcacctcttc atagagggca tcggtatgcg cccaggcggc gaggatgcct ttttcccgga
     2641 gccagtcggc caggtcgagg gcgcccggga gttcgggggc cgcactccag cggcggatcg
     2701 acggaaagcg ggtagtcagc tcccggtatt ccgccgggtc ggggttgcga atatagcggg
     2761 ggtcctgtgc gccacgctgg ttcagcgcga aatacggccc ttcgaggtgc atcccgatga
     2821 accgggcgcc cacccccggc tgcccacaga cgcgatcata gctctccagc gtgtttagca
     2881 gatcctctct tccggcgctg agcgtcgtcg
//
LOCUS       DATGFG010000190         4833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1222966, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000190 DATGFG010000000
VERSION     DATGFG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222966"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..2839)
                     /gene="carB"
                     /locus_tag="VKQ52_03520"
     CDS             complement(14..2839)
                     /gene="carB"
                     /locus_tag="VKQ52_03520"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HLZ86280.1"
                     /translation="MPKDPSIHSVLIIGSGPIIIGQACEFDYSGTQAARSLREEGVEV
                     ILINSNPATIMTDPMMADKVYLLPLTVESIEQILEENKIDAVLPTMGGQTALNLAKEA
                     EDLGVWERHGVRLIGVDIKAIDKAEDREKFRQWMIELGVPVATAKTANSFLEGKEFAQ
                     DIGFPLVIRPSFTLGGTGGGFVHDKDDLDEALNRGLQASPIHEVLVEKAVLGWKEFEL
                     ELLRDANNNVVIICTVENFDPMGVHTGDSITVAPAMTLSDTAFQLMRNTAIRMMRDLG
                     NFAGGCNVQFALNPKTEEIIAIEINPRVSRSSALASKATGYPIAKIAAKLAIGYNLDE
                     LKNQITGTTSAYFEPVQDYVIVKVPRWNFDKFKGANDTLGLQMKSVGEVMAIGRSFTE
                     AVQKACQSLENNAVGLGYYGKSLMHAEELLEYIKTPKWDRIFRIKDALMAGISVGTIS
                     KATNGIDRWFLYEIQKICNMEKDLAKYRIDTLPTDLLGEAKRLGFSDAQIVRIMGSGT
                     EDTLYEKRKAAGITRIFKMVDTCSAEFEAKTPYFYSTFEGGHANESKRSDKKKIIVLG
                     SGPNRIGQGIEFDYCCVHGLLAIKECGYEAIMVNCNPETVSTDFDIADKLYFEPVYWE
                     HLWEIIEHEKPEGVIVQLGGQTALKLAERLTEKGIRIIGTSFDSMDIAEDRGRFSDML
                     KELNIPYPEYGTAFDVDEAIQVANRVGYPVLVRPSYVLGGQRMRIVINDDEVEKAVVS
                     LLKHIPGNKILVDHFLDRCQEAEVDAIFDGETFHVMGVMEHIEPAGIHSGDSHAVLPA
                     FNLTPLEVTTLEYYSEKIARALNIKGLINIQFAIKNGKVYVIEANPRASRTTPFIAKA
                     YQIPYLNIATKIMIGDHKLGDFTFEKKLKGFAIKEPVFSFNKFPGVNKELGPEMKSTG
                     EAIRFIKDLRDPNFRQLYKDRSMYLSK"
     gene            complement(3010..3228)
                     /locus_tag="VKQ52_03525"
     CDS             complement(3010..3228)
                     /locus_tag="VKQ52_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86281.1"
                     /translation="MKGVKQRMVVFLAVTVGGMIGVIKGEEAKQKWVFRMKSKYENRR
                     LMAERKSIARKGGVLLDDIELAAYHNNN"
     gene            3655..>4833
                     /locus_tag="VKQ52_03530"
     CDS             3655..>4833
                     /locus_tag="VKQ52_03530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLZ86282.1"
                     /translation="MKYFVLAAGLCCSLSCFSQFFPPADYPLGYFRDPLDIPMSLAAN
                     FGELRPNHYHMGLDIRTQHRENLPVAAAADGYVARVSVEPGGFGQAIYINHPNGYTTV
                     YGHLNQFFPALAAYVREQQYRRQSWQVNLQLPPTLFPVKKGAFIAYSGNTGGSQGPHL
                     HFEIRRTAGDVNQNPLLFGLPVPDNTPPTLQRLAWYDRNQGIYEQSPHILPIHRGAAS
                     PAHRGPGTPWSTAHWSPTPSLLIVPTTRISFALSAFDTQSGSANPNGIYEAGLFVDEA
                     PVIGFRMNNISYDNTRNINAHIDYRTREMGGPYLQQLFLLSGYPEPSIYAWPAGRPAD
                     GVVRLEDGRVHAIRIVVKDTYGNASVLQFRVQYRAGAGVEGGGAATERIERTAGVAGA
                     G"
BASE COUNT          924 a         1298 c         1405 g         1206 t
ORIGIN      
        1 tggccggcga cggctattta ctcaggtaca tgctcctgtc cttgtacagc tgccggaagt
       61 tgggatcgcg caggtccttg ataaaacgga tggcttcccc ggtggacttc atttcgggcc
      121 ccagctcctt gttgacgccc gggaatttgt taaagctgaa tacgggctcc ttgatggcaa
      181 agcccttcag cttcttctcg aaagtgaagt cgcccagttt gtgatcgccg atcatgatct
      241 ttgtcgcgat gttgagatag gggatctggt aggcctttgc gatgaacggg gttgtccggg
      301 aagcccttgg gttggcttcg atgacgtata ccttgccgtt cttgatggcg aactggatat
      361 tgataagccc cttgatattg agtgcgcggg cgatcttttc cgaatagtat tccagggtcg
      421 tgacctccag gggggtgagg ttgaaggcgg gcagtacggc atggctgtcg ccgctgtgga
      481 taccggccgg ctcgatatgt tccattactc ccatcacgtg gaaggtctcg ccgtcgaaga
      541 tggcgtccac ctctgcttcc tggcagcggt cgaggaagtg atcgaccagg atcttattgc
      601 cggggatatg cttcagcagg ctgactacgg ccttttctac ttcatcatca ttgatgacga
      661 tacgcatccg ctggccgccg aggacatagc tcgggcgtac gaggacggga taacccacgc
      721 ggttggcgac ctggatggct tcgtcgacgt cgaaggccgt gccgtattcg gggtagggga
      781 tgttcagctc tttcagcata tcgctgaagc ggccgcggtc ttcggcgatg tccatgctgt
      841 cgaaggaggt gccgatgatc cggatgcctt tttcggtgag gcgttcggcc agtttgaggg
      901 ccgtctggcc gccgagttgg acgatcacgc cttcgggttt ctcatgttcg atgatctccc
      961 agagatgttc ccagtaaacg ggttcgaaat aaagtttgtc ggcgatgtcg aaatccgtgg
     1021 acaccgtctc ggggttacag ttgaccatga tggcctcgta gccgcattcc ttgatggcga
     1081 gcagcccgtg gacacaacaa tagtcgaact cgatgccctg gccgatgcgg ttgggaccgc
     1141 tgcccagcac gatgatcttc tttttatcgc tgcgcttact ttcattggca tggccgcctt
     1201 cgaaagtgga atagaaatag ggggtcttgg cctcgaactc ggccgagcag gtgtctacca
     1261 tcttaaagat acgggtgatg ccggccgcct tgcgtttttc gtacagggtg tcctcggtgc
     1321 cggagcccat gatcctgacg atctgcgcat cgctgaatcc gagccgcttg gcttccccca
     1381 ggaggtcggt gggaagggtg tcgatccggt atttcgcgag gtctttttcc atgttgcaga
     1441 tcttctggat ctcatagagg aaccagcgat cgatgccgtt cgtggctttg gagatggtgc
     1501 cgacggagat accggccatc aatgcatcct tgatgcggaa gatgcggtcc cacttcggtg
     1561 tcttgatata ttccagcagc tcttcggcat gcatgaggct ctttccgtag tagccgaggc
     1621 cgacggcatt gttctcgagg ctctggcagg ccttctgtac ggcttcggta aaacttctgc
     1681 cgatggccat cacttcgccg acgctcttca tctgcaggcc gagggtatcg ttggcgccct
     1741 tgaacttgtc gaagttccag cggggaactt tcacgatgac atagtcctgt acgggttcga
     1801 aataggccga agtcgtcccg gtgatctggt tcttcagttc gtcgaggtta taaccgatgg
     1861 ccagtttggc tgctatcttg gcgatgggat agcccgttgc cttggaggcc agcgcggagc
     1921 tgcggcttac acgggggttg atctcgatgg cgatgatctc ttccgtcttt gggttcaggg
     1981 cgaactgtac gttgcagccg ccggcgaaat tacccagatc ccgcatcatc cggatggcgg
     2041 tgttgcgcat cagctggaag gccgtatcgc tgagggtcat tgccggcgcc acggtgatgg
     2101 agtcgccggt gtgaacaccc atcggatcga agttctctac ggtgcagatg atgactacat
     2161 tattattagc gtcgcggagc agttccaatt cgaactcctt ccagccgagt acggccttct
     2221 cgaccagcac ttcgtggatg ggggaagcct gcaggccccg gttcagggcc tcgtcgaggt
     2281 cgtccttgtc gtggacgaag ccgccgccgg tgccgccgag ggtgaaggag gggcggataa
     2341 cgagcgggaa gccgatatcc tgtgcgaact cctttccttc gaggaaggag ttggctgtct
     2401 tcgccgtcgc cacgggtacg ccgagttcga tcatccactg tctgaacttc tcgcggtctt
     2461 cggccttgtc gatggctttt atgtctacac cgatgaggcg gacgccatgc ctttcccaga
     2521 cgccgaggtc ttcggcttct tttgcgaggt tgagggccgt ctggccgccc attgtcggca
     2581 gcacggcgtc tatcttgttc tcttccagga tctgttcgat gctttccacc gtcagcggca
     2641 gcaggtaaac cttatccgcc atcatcggat ccgtcatgat ggtggccggg ttactgttga
     2701 tgaggatcac ttcgacccct tcttcccgca agcttcgggc ggcctgggtg ccggagtaat
     2761 cgaattcgca ggcctgaccg atgatgatag gacctgatcc gatgatcagg acggagtgaa
     2821 tggagggatc tttgggcatt tgtggaaaat aaattgcgca aaggtacgat agagtgaggg
     2881 tccctccaaa agaattaccc cgtggaacga aaaagccccg gcagacgccg gggcttgtat
     2941 aactaacaat ttacagggta agtgactcag ttgttaacga aacgaaaggg acccgaaggg
     3001 gactttcgtt cagttattat tgtgataggc tgcgagttcg atatcatcga gcagcacccc
     3061 acccttgcgg gcaatggatt tacgctccgc catcagccgc cggttctcgt acttgctttt
     3121 catacggaac acccacttct gttttgcttc ttcgcctttg ataacgccaa tcatgcctcc
     3181 gacagtcact gccagaaaaa ctaccattct ttgtttaact cctttcatag ctggttcttt
     3241 tttgatggtg aagccttctt cttataaaga cactattagt tagccaaaag ctgtaccaaa
     3301 ctttaaaaaa aacctaccct ttttgtagta ttttttgtag cgacggattt caaggttgat
     3361 tggtaaccgg ttattaacaa tcgcttcact tttgcctgcc ttatctacga aaaaaccgcc
     3421 cgcgcggatt acgatatttt gctttttgtc gtgtcttgtc gtgtcaccaa agcgggcgga
     3481 gcccgcttac accgacatta tgcttttggc ggcaaaaaat taggttctgg aaattttttg
     3541 ctgccaaaat tgggaaggat cggctcacaa tgggaaattt tggcaatcgg gcttcattcc
     3601 cgccgcggct gggtatcttt gctgccgcag catttttaat cctgtgttaa tctcatgaag
     3661 tattttgttc tcgcggctgg tttgtgttgt tcgttatcct gtttttccca gttcttcccg
     3721 ccggcagact atcccctggg ttatttccgg gacccgctgg atatcccgat gagcctggcc
     3781 gccaattttg gcgaactgcg tcccaatcac tatcacatgg ggctcgatat ccgcacccag
     3841 caccgggaga acctgccggt cgccgccgcc gccgatggtt atgtcgcgag ggtatccgtg
     3901 gagcccgggg gttttggcca ggcgatctat atcaatcatc ccaatggcta caccaccgta
     3961 tacggtcacc tcaaccagtt ctttcccgcg ctggcggcct atgtacgcga gcagcagtat
     4021 cgccggcagt catggcaggt gaacctgcag ctgccgccta cgcttttccc tgtgaagaag
     4081 ggagcgttca tcgcgtacag tgggaatacc gggggctcgc agggtccgca tctgcatttc
     4141 gagatccgtc gcacggcggg ggatgtcaac cagaatccat tgctctttgg ccttcccgtg
     4201 cccgacaaca ctccgccgac gctccagcgg cttgcctggt atgaccggaa ccagggtatc
     4261 tatgaacagt cgccgcatat tctacccata caccggggtg cggcatcccc ggcgcatcgg
     4321 ggccccggca ccccgtggag tacggcacac tggtcgccca ccccctccct gctgatcgtt
     4381 cccaccaccc gcatcagttt tgcactgtcg gccttcgata cacagagcgg ttcggccaat
     4441 cccaatggca tctacgaggc cggtctgttt gtcgacgaag cacccgtgat cggtttccgg
     4501 atgaataata tcagctatga caacacccgc aacataaatg cgcatatcga ttacaggacg
     4561 cgggagatgg gtggacccta cttacaacaa ttgttcctgc tatccggcta ccccgaacct
     4621 tccatctatg cctggccggc gggacgaccg gccgatggtg tcgtgcggct ggaggatggg
     4681 cgcgtacatg cgatccggat agtggtgaag gatacgtatg ggaatgcttc ggtgttgcag
     4741 ttccgggtgc agtaccgggc gggcgcaggc gtagaaggag gtggcgcggc gacggagcgt
     4801 atagagagaa cggcgggagt ggcgggtgcg ggg
//
LOCUS       DATGFG010000191         6900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1127711, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000191 DATGFG010000000
VERSION     DATGFG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127711"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VKQ52_03535"
     CDS             complement(<1..652)
                     /locus_tag="VKQ52_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86283.1"
                     /translation="MNQGRIGWVVYLAGTFFFVTVWTGCHSYTRNVSHKNVPLESIRK
                     GEALAGVYCQSCHLLPEPSLLDSRSWEKGVLPAMGPRLGIFHHDLEMYPSSRKDTNLD
                     KSFYPSAPLMSDEEWQHILDYYTATSPDSLPGQERQRPIRRGLSLFRAEAPPLGRPTP
                     ATSLVKMDTGLGGHELWVFDVHYKSLYLYDSRLEQVDSVPDKGAIVDIVRQGAGLER"
     gene            complement(660..4199)
                     /locus_tag="VKQ52_03540"
     CDS             complement(660..4199)
                     /locus_tag="VKQ52_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HLZ86284.1"
                     /translation="MLIVSCQNRQRMFEMIPSAHSGVHFNNTITETDSVNVMDISNVY
                     NGGGVGIGDFNNDGKPDLYFTGNQVPNRLYLNRGDFRFEDVTEAAGVGGDGRWCRGVA
                     VVDINNDGLPDIYVCTTIKTEGKERENLLYVNQGTDKAGVPHFREMAKEYGLADSGYA
                     TQAAFFDYDNDGDLDVYIAENDIISGDYPNHYRPIKKDGSHPSTGRLYRNDWNDSLKH
                     PVFTNVTRQAGTTIEGYAHAVTIADINKDGWKDIYVSNDYLSNDLLYINNHDGTFSEQ
                     LSTYFRHTSANAMGNDIEDINNDGLADVIALDMNPEDNYRKKTMMGAGNYQNYLNNDL
                     FGYSYQYVRNSLQLNLGPRVGRNDSIGPPIFAEIGFFAGIAETDWSWTPMVADFDNDG
                     YRDIIITNGFPKDVTDHDFLAFRDKAYNLVSKKELLKQIPEVKLHKYAFKNNGDLRFS
                     DVSADWGLTTTAFSNGAAYADLDGDGDLDFVVNNINDEAMVFRNNSRELDGKNHHFLQ
                     VELSGDTANRHGLGAWVELHYGGGKQQVYENTPYRGYLSTIQDIAHFGLGADASVDSV
                     VVKWPNGKMQVLRGVAADQVVKVDIRNAGQRYSFEQPPTAVNTLFRDITDSVHLQYRH
                     EGRDYIDFNIQKLLPHKLSQYGPAIATGDINGDGLDDLIVGGASFHSAQVFLQGSDGR
                     FTQTALLPDSVISTKKGDDEGLLLFDADGDGDLDLYIAGGGYRYDRNSDAYADRLYVN
                     DGKGHFTPDDKALPANFTSKFCVRAADYDHDGDLDLFISGRVDPWHYPEPVSSYIYRN
                     DSRNGHIQFTDVTSTVAKDLVRLGMVCDAIWTDFDGDGWPDLILAGEWMPVTFLRNDK
                     GIFKNVTSSTGVGRQTGWWNSIVAGDFDNDGDIDYVVGNLGLNSYYKASEKYPVRAYA
                     GDFDKNGVYDMILSLYLPDTRGEKQEFPAQTRDDLLKQMTAMRKKFPDYKSYGLATMD
                     QVLSPDQRAGALVVKATDFASCLLRNEGGGKFSLIPLPVEAQLSDLDGMVAGDFDGDG
                     KLDLVLSGNDYGTEPTVGRYDAFSGLYLKGDGTDHFTPWPILRSGLYLPGDQKALVRM
                     QGAGGTCLLAASQHQGPLQVFRANRNKRCLPVLPRDISALITYRDGSVRKEEFSYGSS
                     FLSQSGRFLSLDGPIVSVEIEDASGRKRKVKIQ"
     gene            complement(4372..6171)
                     /locus_tag="VKQ52_03545"
     CDS             complement(4372..6171)
                     /locus_tag="VKQ52_03545"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86285.1"
                     /translation="MKRFNYNIIFISSICLVILLYACGKGFLSKPPVGTYQPGTVANK
                     AGVEGLLIGAYSLLDGQGGIGGSWDAAADNWVWGGVCADDAHKGSDPGDQPDIVSLMT
                     WSESSTNSYNLAKWQTCYDGIQRCNETIRIMRLAKDLTPADTMQISAEARFLRGHYHF
                     ELKKVYNMAPYVDETVTYQAGNWRVPNSTDIWPKIEADFQYALTNLGATGYNGSVGRA
                     NKYAAEAFLAKAYMFEHKYAQALPLLNDLITNGETAGGLKYGLVANFNDNFNAATKNN
                     SESVFAVQNSVNDGSTAANANAGDVLNFSYDPAAPVSCCGFYQPSYSLANAFKTDPVT
                     GLPMPSTYNNVDLKNDEGLKSSDAFTPDNTITLDPRLDWTVGRRGIPFLDWGINPGFL
                     FVRNQASGGPYTPIKNAPYASQIGALTDKSSWTAGYTALNVNLIRFADVLLWAAEAEV
                     EAGSLDNAEKYVNMVRNRAANPAGFVHTYVDPTKPQGGFTSTPAANYFIKAYPAGYFT
                     AQGQTTARNLVRFERRLELAMEGHRFFDLVRYGTADVELNAYVAHEVSSGYSLLSGAS
                     FKKGKAEYFAIPQSEIDVSTVGGKPTLTQNPGY"
     gene            complement(6206..>6900)
                     /locus_tag="VKQ52_03550"
     CDS             complement(6206..>6900)
                     /locus_tag="VKQ52_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86286.1"
                     /translation="RNSVGHPISAFYGYQVAGYFKDANDVSKSPTQADAAPGRFKYAD
                     LNHDGKISDSDRTFFGNPNPKFTYGLTLNASYKSFDATIVFYGSYGNDAINYTRYWTD
                     FWASFQGNKSKNLLYNSWSTTNLNPKAPILENASTFSTNTVPNSFYKESASFFKCRSL
                     IIGYNMAPDLLKKAGIDRFRLFVQGSNLFTITKYTGLDPELSGNTQAFGIDFGNYPNN
                     QRTYIVGVNLGF"
BASE COUNT         1642 a         1958 c         1641 g         1659 t
ORIGIN      
        1 ctctctccag acccgcaccc tggcgtacaa tgtcaacgat ggccccttta tcgggaactg
       61 aatccacttg ctcgagcctc gagtcataca ggtaaaggct cttatagtga acatcaaaaa
      121 cccataattc gtgccccccc aacccggtgt ccatctttac caggctggtg gcaggcgttg
      181 gccgtcccag gggcggcgcc tcagcgcgaa aaagcgataa ccccctccgg ataggccgct
      241 gccgctcctg cccgggcaag gaatccgggg aagtggcagt atagtagtcg aggatatgtt
      301 gccattcctc atcactcata agcggcgccg aaggataaaa gctcttatcc aggttggtat
      361 ccttccggct ggaagggtac atctccaggt catgatggaa tatacccagc cggggaccca
      421 tcgccggcag cacgcccttc tcccagctcc ggctgtccag cagcgaaggc tcaggaagaa
      481 ggtgacagga ttgacaatat acgccggcta acgcttctcc cttcctgatg ctttcaagag
      541 gaacgttttt gtgagaaacg ttccgcgtat acgaatgaca gccggtccac accgttacaa
      601 agaaaaacgt accggccaga taaacaaccc accctattcg cccttgattc atttttcgat
      661 tattggattt ttacttttcg cttcctgccc gacgcatcct ctatttcaac cgatacgatg
      721 ggtccgtcca ggctcaggaa tctgccggac tgggaaagga aagaggatcc atatgagaat
      781 tcctccttgc ggacagatcc gtcccggtag gtgatcaacg cacttatatc ccgcggcaac
      841 acaggcagac atcgtttgtt tcggttcgcc cggaatacct gtaacggtcc ctggtgctgg
      901 ctcgcagcca acaggcatgt gccccccgcc ccttgcatcc tgaccagtgc tttctggtcg
      961 cccggcaaat aaagaccgct cctcagaatg ggccagggtg taaaatgatc cgtcccatcc
     1021 cccttcaggt agaggccgct gaatgcgtca taccgcccga cagtgggttc agtcccataa
     1081 tcgttaccgc tcaggacaag atccagttta ccatccccgt cgaagtcccc cgccaccatc
     1141 ccgtcgaggt cggacaactg ggcctccacc ggcaacggga taagggaaaa tttaccaccg
     1201 ccctcgtttc ttaacaggca actggcaaag tcggtagctt taacgacaag ggcgcccgcc
     1261 cgctggtcgg ggctcaacac ctgatccatg gtagccaggc catacgattt gtaatccggg
     1321 aatttctttc tcatggcggt catctgtttc aaaagatcat ccctcgtctg cgcgggaaat
     1381 tcctgtttct ccccccttgt atcaggcaaa taaagcgata agatcatatc gtatacgccg
     1441 ttcttgtcaa aatcccccgc atacgccctc accgggtatt tttcacttgc cttataatag
     1501 gaattcaatc caaggtttcc gaccacatag tcgatatctc catcattgtc gaaatcccct
     1561 gccacaatac tgttccacca cccggtctgc ctgccgaccc ctgtcgacga tgtaacgttt
     1621 ttgaaaatac ccttgtcatt ccggagaaag gtcaccggca tccattcccc ggccaatatc
     1681 aggtcgggcc agccgtcgcc atcgaaatcc gtccagatcg catcgcaaac cattcccagc
     1741 ctgacgaggt ccttggccac ggtcgaagta acatccgtaa actggatgtg cccgttcctg
     1801 gaatcgtttc ggtaaatata gctggacacg ggttcaggat aatgccaggg atcgacccgg
     1861 ccggaaatga ataggtcgag atcgccgtca tgatcatagt cggccgcgcg tacacagaat
     1921 ttgctggtaa aattggcggg aagcgcttta tcatcgggcg taaaatgccc tttcccgtcg
     1981 ttgacataaa ggcggtcggc ataagcgtcg ctattcctgt cgtatcgata ccctccgccc
     2041 gcaatataca ggtcaaggtc gccgtcacca tcggcgtcaa acaataacag cccctcatca
     2101 tccccctttt tagtggagat tacactgtcg ggtagcaacg ctgtctgtgt gaaacggccg
     2161 tcgctgccct gaaggaacac ctgggcgcta tgaaaggaag cgccaccgac gatcagatcg
     2221 tcgagtccgt caccattgat atcccctgtg gcgatcgcgg gcccatattg cgataactta
     2281 tggggcagca gcttctggat attgaaatcg atgtaatccc gtccttcatg ccggtattgg
     2341 agatgcacgg agtcggtgat gtcccggaac aaggtgttga cggcagtcgg cggctgctcg
     2401 aaggaatacc tttgtccggc attccggata tccactttta ccacctggtc cgccgccaca
     2461 ccacgcaaga cctgcatctt cccattcggc cattttacca ccaccgaatc gaccgaagca
     2521 tcagccccaa gtccaaaatg agcgatatcc tgtatcgtcg acaggtaacc cctgtacggc
     2581 gtattctcat agacctgctg ctttccgccg ccataatgca attccaccca ggcgcccaac
     2641 ccatgcctgt ttgcagtatc ccccgacagc tcaacctgca gaaagtgatg gttcttcccg
     2701 tccaactccc gggagttatt ccgaaagacc atggcctcat cattgatatt gttgacgaca
     2761 aagtccagat cgccatcgcc atccagatcc gcatacgccg ccccgttgga aaaagccgtc
     2821 gtcgtcagcc cccaatccgc cgatacatcc gaaaaacgca ggtccccgtt atttttgaat
     2881 gcatatttgt gcagcttcac ttcgggtatc tgctttagca gctctttttt tgagacgaga
     2941 ttatacgcct tgtccctgaa agccaggaaa tcatgatcgg tgacatcctt tggaaaccca
     3001 ttcgtgataa taatgtcgcg gtaaccgtca ttgtcaaaat ctgcgaccat cggcgtccag
     3061 ctccaatccg tttccgcgat gcccgcaaaa aatccaatct cggcgaagat gggggggccg
     3121 atggaatcgt tccggccgac cctgggaccc aggtttaatt gcagcgaatt ccggacatat
     3181 tgataggaat agccgaaaag atcattgtta aggtaattct gataattacc agcccccatc
     3241 atcgtcttct tacgataatt gtcttccggg ttcatgtcca gcgcaatgac atccgcaagc
     3301 ccatcattgt tgatgtcctc gatatcattt cccatcgcat tggccgacgt atgcctgaag
     3361 taggtagata attgttcgga aaaggtgccg tcgtgattgt tgatatataa caggtcgttg
     3421 gaaaggtagt cattgctgac atagatatcc ttccagccat ccttattgat gtctgcaatg
     3481 gttaccgcat gcgcataccc ttcgatcgta gttccggcct gccgggttac attggtaaat
     3541 acaggatgct tcagtgaatc gttccaatcg ttccggtata acctgcctgt actgggatga
     3601 gagccgtcct tcttgatagg ccggtaatga ttgggatagt cccctgaaat gatatcattt
     3661 tcggcaatgt acacgtcgag atcgccatcg ttatcataat cgaaaaaggc cgcctgggtc
     3721 gcatatccgg aatcggccag cccatattcc ttcgccattt ccctgaaatg cggaacgcct
     3781 gccttatcgg tgccctggtt gacatacaaa aggttttccc gttcctttcc ttccgttttg
     3841 atcgtcgtgc acacataaat gtcaggcagg ccgtcgttat ttatatcgac caccgcgaca
     3901 ccccggcacc atcttccgtc gccgccgaca cctgccgcct ccgtgacatc ttcaaaccga
     3961 aaatcacccc tgttcagata taaccggttc ggcacctgat ttccggtaaa atagaggtcg
     4021 ggcttcccgt cattattgaa atccccaatt cccacgccgc cgccattata gacattggaa
     4081 atgtccatca cgtttaccga atccgtctcc gtaatggtat tattgaaatg gacaccggaa
     4141 tgcgcagatg ggatcatctc gaacatccgc tgccggttct ggcaggatac gatcagcaat
     4201 atgactgcta aaacaggccc ggttttccaa aatcggctaa ccaattccat gtagtatgat
     4261 ttcttggtca atatagttaa aaaaaaagac cggccccgaa cagaaatggg gccagtctat
     4321 ctttatgtag tcaaaaaatt cagctgccgg aagcaatcca ttcccacatt gctaataacc
     4381 tggattctgg gtcagggtcg gcttaccgcc gacagtgctg acatcgattt ccgactgtgg
     4441 gatcgcaaag tattcagcct tgcccttctt aaaactggcg ccgctcaaca ggctataccc
     4501 ggacgacact tcatgggcaa cataagcatt taattcgaca tcggctgtgc cataccggac
     4561 gaggtcgaag aaccgatgtc cttccatggc caattccagc ctccgttcaa accggaccag
     4621 gttgcgcgcc gtagtctggc cctgtgcagt aaaatatccc gcgggatatg cctttatgaa
     4681 ataattcgca gccggagtac tcgtaaagcc cccctgcggc ttggtgggat ctacatacgt
     4741 gtgtacaaaa cccgccggat tcgcagccct gttccggacc atattcacat atttctccgc
     4801 attgtccaga ctgcctgctt ccacttccgc ctcagcagcc cacaacaaga catccgcaaa
     4861 ccggatcagg ttgacattca gcgcggtata cccagccgtc caggaagact tgtctgtaag
     4921 tgcaccgatc tggctggcat atggcgcatt cttgatgggc gtgtacggtc cgccgctggc
     4981 ctgattacgg acgaacaaaa agccgggatt gataccccaa tcgaggaagg gaatgcctct
     5041 ccggcctacc gtccaatcca gccggggatc cagggtaatg gtattgtcgg gcgtaaaggc
     5101 atctgacgat ttcaagccct catcattctt cagatccaca ttattgtacg tgctgggcat
     5161 cggcaatccc gtcaccggat cggttttgaa cgcatttgcc aggctatagg acggctgata
     5221 aaatccacaa caggaaacgg gcgccgcagg atcgtaagag aaattaagga catcccctgc
     5281 attcgcattg gctgccgtcg atccgtcatt caccgagttc tgcacggcaa atacggactc
     5341 actgttgttc tttgtcgccg cattgaaatt gtcattgaaa ttagccacca ggccatattt
     5401 caggccgccg gccgtttctc cgttggtgat caggtcattc agcaggggca gggcctgggc
     5461 atacttatgc tcgaacatat aggccttggc aaggaacgcc tcagccgcat atttgttggc
     5521 gcgaccaact gagccgttat accccgtcgc gccgaggttg gttaatgcat attgaaaatc
     5581 agcctcgatc tttggccaga tatccgtgct attcggtacc cgccagtttc cggcctgata
     5641 ggtaaccgtt tcgtccacat agggggccat attgtagacc ttcttcaact caaaatgata
     5701 atgcccccgg aggaacctgg cttccgcgct gatctgcatg gtgtctgccg gcgtaagatc
     5761 cttggccagc cgcatgatcc ttatcgtttc attacaacgt tggatcccgt cataacaggt
     5821 ttgccacttg gcaagattat agctgttggt cgaactctca ctccaggtca tcagggaaac
     5881 gatgtccggc tggtcgcccg gatccgaacc tttgtgggca tcatccgcac aaacaccgcc
     5941 ccaaacccag ttgtcggcag cggcatccca ggacccgccg atacctccct gaccatccaa
     6001 aagagagtaa gcgccgatca gcaacccttc gacacccgcc ttgttggcta cggtacccgg
     6061 ctgataagta ccgacaggtg gcttgctcaa aaatcccttc ccacaggcat acaaaagtat
     6121 taccaggcaa atactggaaa tgaatattat attataattg aatcttttca tatactattt
     6181 tttgtgtatt ccgactaatg gttatttaga atccaaggtt gacgccgacg atatacgtgc
     6241 gttgattatt cgggtagttc ccgaaatcga tgccaaaggc ttgcgtgttg cccgataatt
     6301 ccggatcaag ccccgtgtac ttcgtaatgg tgaaaaggtt cgaaccctgg acaaatagtc
     6361 tgaaccggtc gatacctgcc tttttcaata agtccggagc catattgtaa ccgatgatca
     6421 gcgagcggca cttgaagaaa gaggcgcttt ccttatagaa ggaattggga acggtgttgg
     6481 tgctgaacgt ggatgcattc tcgaggatcg gggcctttgg attcaggttg gtcgtcgacc
     6541 aggaattata cagcaggttc ttgctcttat tcccctggaa agaagcccag aagtcggtcc
     6601 agtaccgggt ataattgatc gcatcattac cataactgcc atagaacacg atggtcgcat
     6661 caaaactttt ataagaagca ttgagggtca acccataggt gaatttcgga ttggggttgc
     6721 caaagaacgt ccggtcgctg tccgagatct tcccgtcatg attcaggtcc gcatatttga
     6781 acctgcccgg cgcggcatcc gcctgggtcg gcgacttgga gacatcgttc gcatccttga
     6841 aatatccggc cacctggtag ccataaaagg cgctgatggg atggccaacg gaattccgga
//
LOCUS       DATGFG010000192         6110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_312962, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000192 DATGFG010000000
VERSION     DATGFG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312962"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2948)
                     /locus_tag="VKQ52_03555"
     CDS             complement(<1..2948)
                     /locus_tag="VKQ52_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86287.1"
                     /translation="MLLAFLLVCAHTFAQPNERKITGRVTDANTGLPVAGASVAVKGG
                     QNAVTSDADGVFSITAKTGQTLDITAIGHISKSLKVDAETNAFAIQLDQSYAKLDDVV
                     VVGYGVQKSKAITGATAHVKGEDLAKLNTTNALQALQGQAAGVNITSTSGQPGGGFKV
                     NIRGAGTIGNANPLYVVDGVISSDITYLNPADIASVDVLKDAASSAIYGINGANGVIL
                     ITTKSGKMGGKNSGQISFDGYYGYQNAPPKTSLLNAQQYAVMQNEAALNSGLPALFTQ
                     AQINGLGKGTNWLDQMIASNVPAQNYSLSATGGSDISSYSLGLSYTQQGGIVGGEKLS
                     NYQRYNFRANSERKMYDGALKVGEHLTFSFIDQYGIQDGGIYGNTLRSAFTTSPLLAM
                     YDSAGHYLSSLKSTVYNGGPWNTQEANPYALMVYNNQNDNKTQKFFGDVYAELQPVKN
                     VKFRTTLGLNYSSSANHSYTPVYDNLSVYAYNRYETINQGSSVSYTVNWDNTANYTFR
                     IKEHSFDVLAGSATRKYQGSFVGGMNTGSTLFSTFDRGYLSTSNVTSISMSADTTTAN
                     KQTVTHAMSLYGNANAIYAQASFFGRINYSYKETYLASAIFRADGSTMFAQNHQWGYF
                     PSLSVGWVLTNESFMGSTRDWLNFLKIRASWGSNGNDGITAFNYLSLISLSNAQYNFG
                     NNNTSLTNGSYPSTIGVDKTKWETSRQTNIGIDAQMLNNKLSASIDLYNKLTKDWLVA
                     APLLATAGVSTNPYINGGNVTNKGIELQLAYNDRIGKDFSYSISGSYAYNENKVTNIP
                     TADGIIHGGTNSLFVNAPEFFRASSGNPIGYFWGYKTAGIFQSEADVQAWRGKNGALQ
                     PSAHPGDVKFVDQNGDGVIDAKDKTNIGDPNPHHVFGLRFSGNYKHFDLSITTNGVAG
                     NKLVQSYRDLGAYGNWTTAILSRWHGQGTSNSMPRVTQNNSNWADFSDLYIHDGSYLR
                     ISNITLGYDFA"
     gene            complement(3309..>6110)
                     /locus_tag="VKQ52_03560"
     CDS             complement(3309..>6110)
                     /locus_tag="VKQ52_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ86288.1"
                     /translation="DPDPASLADDRVYAIREDADGALWVGTMTGGLDRLDVEKGKFYH
                     NNMATPYSIQSNYVSALTEDSASNLWIGTAWGIEVLSRSTGKYTHYTTTGNGLTDDVI
                     FAITRDHRGNIWVATRKGLNVLQPGKDTFQVFHMKDGLPSNSVLGILEDNEQRLWVST
                     SGGLSRITVSGNGSHIRINCVNFDEYDGLQGREFAEGAAFRTREGELLFGGGKGFNLF
                     KPESIGIDRHIPPVVLTGLQLYNKEVPLPPSIPMDGKLVLTYKENNFTLDFAALSYIN
                     PRKNHYAYQLMGFDKDWMTTDGRSPRATYTNIDPGEYVFRVRASNSDGVWNDRGVTVR
                     VIVKPPFWKTGLAYAVYVLLILAALYIGRMSIISRARARFALAEERREAVRIHELDRL
                     KIKFFTNLSHEFRTPLSLILSPVDKLIRGTEDPGRRQLAMTIERNAKRLLHLVNQLLD
                     LRKMEVNELKLNMSRGDIGEFIRATTDSFADLAEGKGIDLDYHSDVDHLPALFDRDKV
                     ERIVFNLLSNALKFTSTGGSVVVELMVVEKDEYSALLQLRVTDTGIGIPAELQGKIFE
                     SFFQGETPGHIRNQGTGIGLAITREFVEMHGGTIRVESAQGKGSCFTMLLPVTILAGL
                     STAPAASPIHSPALPPATPPAHSPAATEAEAPVKERMNRPQKMDEPRAPSEGGEKFKL
                     LLVEDDDDFRFYLKENLAPLFIVLEAANGREGWQKTLSAQPDLVVSDVNMPLMDGLTL
                     CGKIKSDDRVRHIPVILLTALSDEEDQLRALGMGVNDYLSKPFNVEILISRIKNLLEF
                     KDNVEQTMKKRVDIAPGEVEVEPERTEADFVREAVAVLEKNMANADFSVEDWSRELGL
                     SRTTLYKRILAATGKTPIGFIRQFRMQRAAQLLEKTRHNVAEVAYMVGFNNPKYFARY
                     FKEVYGKLPSIYQAEKRKG"
BASE COUNT         1240 a         1531 c         1775 g         1564 t
ORIGIN      
        1 gcaaaatcgt atcccagggt gatgttcgag atccgcaggt agctgccgtc atgaatatag
       61 aggtcggaaa aatcagccca gttggagttg ttttgggtca cgcggggcat cgaattggag
      121 gttccttgtc catgccagcg actcaggatg gcagtcgtcc agttaccata agcacctaaa
      181 tccctgtaag actgaaccag tttatttccc gcaacgccgt ttgttgttat ggagaggtcg
      241 aaatgtttat agttgccgga aaaccgcagc ccaaaaacat gatgcgggtt tggatcgcct
      301 atatttgttt tatccttggc gtcgatgacg ccatcgccgt tttggtccac aaatttcacg
      361 tcacccggat gggcggaggg ctgaagagcg ccgttctttc ccctccaggc ctggacgtcg
      421 gcttccgact ggaagatgcc ggccgtttta tatccccaga aatacccgat ggggtttccg
      481 gaggacgcac ggaaaaattc cggggcattg acaaacaggc tgttcgtgcc gccgtgaatg
      541 atcccgtcgg ccgttggaat attggtcacc ttattttcat tataggcgta ggagcccgaa
      601 atcgaatagg agaaatcctt tccgatcctg tcgttgtaag ccagctggag ctctattcct
      661 ttattggtga cgttgccgcc gttgatgtag ggatttgtcg aaaccccggc tgtcgcgagc
      721 agtggcgcgg cgaccagcca gtcctttgtc agtttattat aaagatcgat gcttgcactg
      781 agcttattgt tcagcatttg ggcatcgatg ccgatattgg tttgacgcga ggtctcccat
      841 ttggttttat cgacgccgat ggtggaggga taagagccat tggtcaggct ggtattgttg
      901 ttgccgaaat tatattgggc atttgacagg ctgatcaagg acaggtaatt aaaagcagtt
      961 atcccgtcat taccgttgga cccccagctt gcccggatct tgaggaagtt caaccaatcc
     1021 ctggttgatc ccatgaaaga ttcgttggtg agcacccagc ctacggaaag ggagggaaag
     1081 tatccccatt gatgattctg ggcaaacatg gtggaaccgt cggcacggaa gatggcggaa
     1141 gccaggtatg tttctttgta ggaatagttt attctaccga agaaggaagc ctgggcataa
     1201 atggcattcg cattcccgta gagcgacatg gcatgcgtca ctgtttgttt attggcagtc
     1261 gtggtatccg cgctcatgga tatggaggtg acgttggaag tactcagata gccgcggtca
     1321 aaagtggaga atagagtgga gccggtattc atgccgccca caaatgaccc ctggtatttc
     1381 cgggtggcgc tacccgccaa aacatcaaag ctgtgttctt taattctaaa ggtgtagtta
     1441 gctgtattat cccaattgac ggtatacgac acggagctgc cttgattaat ggtttcgtat
     1501 ctattataag cgtagaccga caggttatcg tagaccgggg tataactgtg gttggcggaa
     1561 gaggagtaat tcaaccccag ggtggtcctg aactttacat ttttcaccgg ctgcaattcc
     1621 gcatagacat ctccaaaaaa cttttgcgtc ttattgtcat tttgattgtt gtagaccatc
     1681 agggcgtaag gatttgcttc ctgggtgttc catgggccgc cgttgtatac cgtggacttg
     1741 aggctgctta ggtagtgtcc ggccgagtcg tacatggcca gcaaggggct ggttgtaaat
     1801 gcgctccgga gggtgttgcc atagatgccc ccatcctgga taccgtattg atcaataaag
     1861 gagaaggtga gatgctcccc tactttcaag gctccgtcat acattttccg ttcagaattg
     1921 gcacggaagt tatagcgctg ataattggaa agtttttctc cgccgacgat accaccctgt
     1981 tgcgtatagg acagtcccaa tgaataggat gagatgtccg acccgccggt ggcggacagg
     2041 ctataatttt gggcgggcac attggaagca atcatctggt ccagccagtt agtccctttg
     2101 cccagcccgt tgatttgcgc ctgggtaaaa agggcgggta gtccggaatt gagtgcggct
     2161 tcattttgca tgacagcata ttgttgtgca ttcaacaggc ttgttttcgg cggagcgttt
     2221 tggtatccgt agtagccgtc aaatgagatc tgcccactgt tcttgccacc cattttcccg
     2281 cttttggtgg tgatcagtat gacgccgttt gctccattga taccgtagat ggcgctggat
     2341 gcggcatctt tgaggacatc gaccgaggcg atgtcggcag gattcaaata cgtgatatcg
     2401 ctggatatta cgccgtctac cacgtataag gggttggcat ttccgatggt gccggcgccg
     2461 cggatattca ctttaaaacc gccgccgggc tgtcccgagg tggaggtgat attgaccccg
     2521 gcggcctggc cctgtaatgc ctgcagggcg ttggtggtgt ttagctttgc gaggtcttca
     2581 cctttgacgt gggcggtggc gcctgtgatg gctttcgact tttggactcc gtagcccact
     2641 acgacgacat cgtcgagctt tgcatagctt tggtccagtt ggatggcgaa ggcatttgtt
     2701 tccgcgtcga cctttagtga ttttgaaata tgccctatag cggtaatgtc aagggtttgg
     2761 cctgtctttg ccgtaatact gaatacccca tcggcgtcgg atgtaacggc attttggccg
     2821 ccctttacgg caaccgaggc gcctgcgacc gggagccctg tattggcatc ggtgaccctc
     2881 ccggttattt ttcgttcatt gggctgcgcg aaggtgtgag cgcataccag cagaaaggcc
     2941 aatagcatca ggattgggtt agagcccttc cttttcgtct tcttttctaa cataaacaag
     3001 caattattgg ttaaataaat agcgggataa ttcgatcgga tgtacgggta tggatcctat
     3061 aggcccgggg atgggctgca ggcggggggc tgccagtaag aggcaaagca attttttgtc
     3121 gtgcatcgca atcggttgta atagtatgta aaattaggcg ggatgatgga aagggggggt
     3181 atggcaactt gcaaatcaag gggttgaatt gttgccgagg ggggtatgat tctgttattt
     3241 ctggggtaaa aattgttcaa aggcggcggg ccgggtttgt gcgggcgggc cgggtttgcg
     3301 cgggcgaatc agcctttgcg tttctctgcc tggtagatgc tgggcagttt gccgtacacc
     3361 tccttgaaat accgggcgaa atatttgggg ttgttgaagc caaccatata tgccacctcc
     3421 gccacgttgt gccgggtctt ttccaggagt tgggcggccc gctgcatccg gaactgacgg
     3481 ataaatccta tgggggtctt gcctgtggcg gcgagtatcc gtttgtaaag cgttgtccgg
     3541 ctcaagccca attccctgga ccagtcttcc accgaaaagt cggcgttggc catattcttt
     3601 tccagcacgg ctacggcttc ccggacgaaa tccgcttcgg ttcgctccgg ttcgacctcc
     3661 acttcgccgg gtgcgatgtc gacgcgcttt ttcatggttt gctccacgtt atccttgaat
     3721 tccaggaggt ttttgatgcg ggagatgagg atctcgacat tgaaaggctt ggacagatag
     3781 tcattcacgc ccatgccgag ggcccggagc tggtcttctt cgtcagaaag cgcggtgagc
     3841 aggatcacgg ggatatgccg cacccggtcg tcgcttttta tctttccgca aagcgtcagg
     3901 ccgtccatca acggcatgtt gacatcgctt accacgaggt cgggctgggc ggaaagcgtt
     3961 ttctgccatc cctcccggcc attggccgcc tcgagcacga taaacagcgg ggcgaggttc
     4021 tctttgaggt agaatcgaaa atcgtcatcg tcttccacca gcagcagttt gaacttttca
     4081 ccgccttcgg atggcgcccg cggttcatcc attttttgcg gtctgttcat tctttccttc
     4141 accggcgctt ccgcttcggt tgcggccggc gagtgagccg gcggggtggc tggcgggaga
     4201 gccggcgagt gaatcggcga ggcggccggc gccgtcgata atccggcaag gatggtgacc
     4261 ggcaggagca tggtgaagca gctgcccttg ccctgagcgc tttccacgcg gatagttccc
     4321 ccgtgcattt ccacgaactc ccgtgtaatg gccaggccga tgccggtgcc ctggttccgg
     4381 atatgccccg gcgtttcacc ctggaagaag gactcaaaga tcttcccctg caattccgcc
     4441 ggtatgccga tgccggtatc ggtgacgcgg agctggagca aggcgctgta ttcgtccttt
     4501 tcgacgacca tcagctcaac gaccactgaa ccgccggtgg aggtgaattt taaggcgttg
     4561 gagaggagat tgaagacgat gcgctcgact ttatcccggt cgaacagggc ggggaggtga
     4621 tctacgtccg agtgatagtc caaatcgatg cccttcccct ctgccaggtc tgcaaaagaa
     4681 tcggttgttg cgcgtataaa ttcccctatg tctcctctcg acatattcaa tttcagctca
     4741 ttgacctcca tttttctaag gtcgagcagc tggttcacga ggtggagcaa tctttttgcg
     4801 ttgcgttcga tggtcatggc cagttgtcgc cggccggggt cttccgtgcc ccggatcaat
     4861 ttatccactg gtgaaaggat cagcgacagg ggtgtccgga attcatggct gagattggta
     4921 aagaatttga tcttcagtct gtccagctcg tgtatccgta ctgcttcccg ccgttcttcc
     4981 gccagcgcga agcgggcccg ggcgcggctg ataatactca ttcgcccgat gtaaagggcc
     5041 gctaaaatga gcagtacata aacggcatag gcgaggcccg tcttccagaa gggcggcttt
     5101 acgatcaccc ggacggtgac tccccggtcg ttccacaccc cgtcgctgtt ggaagccctg
     5161 acccggaaga catactcccc gggatcgata ttggtatacg tggcccgcgg gctgcggccg
     5221 tccgtcgtca tccagtcttt gtcgaatccc attagctggt acgcgtagtg attcttacgg
     5281 ggattgatat agctgagcgc cgcgaaatcc agggtgaaat tgttctcctt atacgtcagc
     5341 accagttttc cgtccatcgg gatcgatggc ggcaagggga cttccttgtt atagagttgc
     5401 agcccggtga ggacaaccgg cggaatatgc ctgtcgatgc cgatggattc gggtttgaac
     5461 aggttgaatc cttttccacc gccgaagagc agctctcctt cgcgggtgcg gaaggcggcc
     5521 ccctcggcga attcgcggcc ttgcaagcca tcgtattcgt cgaagttcac gcagttgatg
     5581 cggatgtggc ttccgttccc ggagacagtg atcctggaaa ggccgccgga ggtgcttacc
     5641 cagaggcgtt gttcgttatc ttccaggatg cccaggacgg aattcgatgg aaggccgtct
     5701 ttcatatgga atacctggaa ggtatccttt cccggctgga gcacattcag tcccttcctg
     5761 gtcgccaccc agatatttcc cctgtgatcg cgggtgatgg caaagatcac gtcgtcggtg
     5821 agcccgttgc cggtggtggt atagtgggta tacttacccg tggaccggct cagcacctcg
     5881 atgccccagg cggtgccgat ccacaggttg gacgcggagt cttctgtcag cgcggacaca
     5941 taattcgact ggatggaata aggcgtagcc atattattgt ggtagaactt acccttttcc
     6001 acatccagtc tgtcgaggcc gccggtcatg gtgccgaccc aaagcgcgcc gtcggcatct
     6061 tcgcggatgg catagacccg gtcatcggcg aggctggccg ggtccggatc
//
LOCUS       DATGFG010000193         4167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_650236, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000193 DATGFG010000000
VERSION     DATGFG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650236"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VKQ52_03565"
     CDS             complement(<1..379)
                     /locus_tag="VKQ52_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLZ86289.1"
                     /translation="MENNYSFIDYAPRRDAELLDGYSRTITGVVGQVAEAVVHIQVQK
                     PAPPVERRNGERGRDGERGRDRSQPLMPASGSGFIISTDGFVVTNNHVIEHAKEIRVS
                     LADGRTVNAELKGADPSTDIAVLK"
     gene            563..2605
                     /gene="paaZ"
                     /locus_tag="VKQ52_03570"
     CDS             563..2605
                     /gene="paaZ"
                     /locus_tag="VKQ52_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HLZ86290.1"
                     /translation="MPKLGNYVTGNWITGDGEGQPLFNAATGDTIAHASTKGLDFAAI
                     LDFARATGNPALRKMTFPERGRMLRALALHLREHLDTFYRISFLTGATRADSWIDLEG
                     GIGNLFSYASLRRKFPDEPFCLDGEGLSLGKGGTFMGQHVLVPKEGAAIHINAFNFPV
                     WGMLEKIAVNLLAGVPAVVKPATVTSFLTEAVVREIIASGILPPGALQLICGSAGTLL
                     DHVTAQDVVTFTGSASTGMQLKAHPRILAESTPFNMEADSLNCLVLGEDVEPGMPEWD
                     LFIKELRKEMTVKAGQKCTAVRRIFVPENKLDEARKALIKAIAQTTIGNPENEKVRMG
                     PLAGQSQREEVRAQVTKLLASSQLIYGSLDSVELVEGDAVRGAFLSPLLLLSETPFTA
                     EEPHSIEAFGPVSTLMPYKDIDAAIALSRKGKGSLCSTIVTADADIARQYTMGAATHH
                     GRILILNNECAKESTGHGSPLPLLVHGGPGRAGGGEEMGGIRGVKHYMQRVAVQGSPS
                     MITHLTHTYQPHAKQTLDEKHPFRKYFEELQIGDTVITHKRTVTEADIVNFANVSWDH
                     FYAHTDHTSLEGTLFEKPVAHGYFILSAAAGLFVDAGKGPVMLNYGLEECRFLKPVYA
                     GSTIGVKLTCKEKIAQEKKEEKDIPRGIVKWYVDVYDETGESVAIATILTMVQRKP"
     gene            2653..3423
                     /locus_tag="VKQ52_03575"
     CDS             2653..3423
                     /locus_tag="VKQ52_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLZ86291.1"
                     /translation="MLKQIKEGHVKSERHNGVLTIEFYHPQSNSLPGVLLEAITHEIH
                     GAGNDNDTKVIILQSGAERAFCAGASFDELLAIETAEQGLQFFSGFAHLINEMRKCPK
                     LIIARIQGKCVGGGVGIAAAADYAIALEGADVKLSELALGIGPFVVGPAVERKIGLSA
                     FSSLSIDATMWRDAEWARRRGLYAELHSTMTGMDESIARLAGHLAHSNPEAMTLMKQT
                     LWKGTEHWDHLLLERAALSGRLVLSAFSKAAIQKLRKK"
     gene            complement(3452..4087)
                     /locus_tag="VKQ52_03580"
     CDS             complement(3452..4087)
                     /locus_tag="VKQ52_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HLZ86292.1"
                     /translation="MTPIEKLLLENKAWSSEIKADDPTYFERLAHIQTPEFLWIGCSD
                     SRVPADKITGTQPGEIFVHRNIANLVIHTDVNLLSVLDFAVNHLKVNHVIVCGHYGCG
                     GVKAAMTNHDYKYVLNMWLRNIKDVYRLYAKELDAISDEEKRADRLTELNVKEQVMHL
                     AKTSIVQRAWKQERRPQLHGWVYGLKDGLIKPVFDMDAGSAIDPIYQYDDL"
BASE COUNT          967 a         1357 c         1060 g          783 t
ORIGIN      
        1 tctttaacac cgcgatatcc gtcgaagggt ccgcaccctt taattccgca ttcaccgtcc
       61 gcccatccgc cagcgatacc cggatctcct tcgcatgttc gatgacatgg ttattggtca
      121 cgacaaaccc atccgtggaa atgataaaac cggaacccga agcaggcatc aacggctggc
      181 tacgatcccg gccccgttct ccgtcccggc cgcgttcgcc attccggcgc tcgaccggcg
      241 gcgccggctt ctgcacctgt atgtgcacga ccgcctccgc cacctggccc actacccccg
      301 tgatggtccg ggaatagccg tccaacagtt ccgcatcacg ccgcggagca tagtcgataa
      361 atgaataatt gttttccata taccaaccgc gacaaaatat tgaccaatct ttttttgctg
      421 ccagtatggc attgccaccg gcacaacttc attccggctt tttgccgggc ggaccgtggt
      481 ccgccggccg aaggcccgac attagccctt tctgcggtcc gtcaaacttt gtatcttaga
      541 cgcggataaa cgattgaaca ctatgccgaa gcttggaaac tatgtgaccg ggaactggat
      601 caccggtgac ggtgaagggc agcctctttt caacgcggcc acgggcgaca ccatcgccca
      661 cgccagcaca aaaggcctgg attttgccgc catcctcgat tttgcccgcg ccacgggcaa
      721 ccccgctctt cgcaaaatga ccttccccga acgcggcagg atgctgcggg ccctcgccct
      781 ccacctccgc gaacaccttg atacctttta ccgcatcagc ttcctcaccg gcgcaacccg
      841 cgccgatagc tggatcgacc tggaaggcgg tatcggcaac ctcttctcct acgcctccct
      901 ccgccgcaaa ttccccgacg aaccattctg cctcgacggc gaaggactgt ccctgggcaa
      961 gggcggaact tttatgggcc agcacgtcct ggtgccgaaa gaaggcgcgg cgatccatat
     1021 caacgccttt aacttccccg tctggggcat gctggaaaag atcgcggtaa acctcctcgc
     1081 aggagtaccc gccgtcgtca aacccgcaac cgtcacctcc tttctcaccg aagccgtggt
     1141 ccgcgagatc atcgcctccg gtatcctccc gcccggcgca ctccagctca tctgtggaag
     1201 cgccggtacc ctcctggacc acgtcacggc ccaggacgtc gtcaccttca ccggctcggc
     1261 atccacgggc atgcagctaa aggcccatcc ccgcatcctc gcggagagca ccccctttaa
     1321 tatggaagcc gactccctca actgcctggt cctcggcgaa gatgtagaac ccggcatgcc
     1381 ggaatgggat ctgttcataa aggagctccg gaaggaaatg accgtgaaag ccggccagaa
     1441 atgcaccgcg gtaagacgca tctttgttcc ggagaacaaa ctcgacgaag cccgcaaggc
     1501 ccttataaaa gccatcgccc aaacgacgat cggtaacccg gagaacgaaa aagtgagaat
     1561 gggccccctc gcgggacaat cccagcgcga agaggtccgc gcccaggtga caaaactcct
     1621 cgcctcctcc caactgatct acggctccct cgacagcgtc gaactcgtcg aaggggacgc
     1681 tgtccgcggc gccttcctga gcccgctgct gctgctgagc gaaacgccat tcacagccga
     1741 agagccgcat tccatcgaag ccttcggccc cgtcagcacc ctgatgccct ataaggatat
     1801 cgacgccgcc atcgcccttt cccggaaagg aaagggatcg ctgtgcagca ccatcgttac
     1861 ggcagacgcg gacatcgccc gccaatatac catgggcgcg gcgacccatc acggccgcat
     1921 cctcatcctc aacaacgaat gcgcaaagga aagcaccggg cacggcagcc cgctgccgct
     1981 gctggtacac ggcggccccg gacgcgccgg cggcggcgaa gaaatgggcg gcatccgcgg
     2041 cgtaaagcat tatatgcagc gcgtggccgt gcagggctct ccctccatga tcacccatct
     2101 cacccacacc taccagccgc acgcaaaaca gaccctcgat gagaaacacc ccttccggaa
     2161 atatttcgag gaactacaga taggagacac cgttatcacc cataaacgca cggtcaccga
     2221 agccgatatc gtcaacttcg ccaacgtaag ctgggatcat ttctacgccc ataccgacca
     2281 cacctccctc gaagggaccc tcttcgaaaa gccggtcgcc catggctatt ttatcctcag
     2341 cgcggccgcc ggcctttttg tcgacgccgg gaaaggaccc gtcatgctca actacggcct
     2401 ggaggagtgc cggttcctga agcccgtcta cgccggcagc accatcgggg tgaagctcac
     2461 ctgtaaagaa aagatcgccc aggagaaaaa agaggaaaaa gacattcccc ggggcatcgt
     2521 aaaatggtat gtcgatgtct atgatgagac gggggagagc gtggccatcg ccactatcct
     2581 tacaatggtg caacggaaac cctgaacaaa aatccctgaa caaaacctga accccaaaaa
     2641 acaaatatcg ctatgcttaa acagatcaag gaaggtcatg tcaaaagcga acgccacaac
     2701 ggcgtcttga ccatcgaatt ctaccatccg caaagtaatt ccctgcccgg agtcctgctg
     2761 gaagccatca cccatgagat ccacggcgcc gggaacgaca acgatacaaa ggtcatcatc
     2821 ctccagtccg gcgccgaacg ggccttttgc gccggcgcct ccttcgatga actgctcgcg
     2881 atcgaaacgg cggaacaggg gctgcaattc ttcagcggct tcgcccacct gataaacgaa
     2941 atgcgcaaat gcccaaaact catcatcgcc cgcatccagg gcaagtgcgt cggcggcggg
     3001 gtcggcatcg cggcggcagc cgattatgcc atcgccctgg aaggcgccga cgtaaagctg
     3061 agtgagctgg ccctcggcat cggccccttc gtggtaggac ccgcggtgga gagaaagatc
     3121 ggcctctccg ccttcagcag cctgtccatc gacgcgacca tgtggcgtga cgccgaatgg
     3181 gcgagaagaa gaggcctcta cgccgaactc cacagcacca tgacgggcat ggacgaatcc
     3241 atcgcccggc tggccgggca tctcgcacac tccaaccccg aagccatgac cctcatgaaa
     3301 caaaccctgt ggaagggaac ggaacactgg gaccacttac tgctggaaag ggcggccctg
     3361 agcggaaggc tcgtgctaag cgctttctca aaagcggcaa tacagaaact caggaaaaaa
     3421 taaaagaggc ggcccggtgg ccgcctctca cttatagatc atcgtattgg tagatcggat
     3481 cgatggccga ccccgcatcc atatcaaaga ccggcttgat cagcccatcc ttgagcccat
     3541 agacccagcc atgaagctgc ggccggcgct cctgcttcca tgcccgctgg acgatggagg
     3601 tcttggccag atgcatcacc tgctccttta cattcaactc cgtaagccgg tcggcccgct
     3661 tctcctcatc ggatatcgca tccagttctt ttgcataaag acggtataca tcctttatgt
     3721 tccgcagcca catattcaac acatatttat agtcgtggtt ggtcatggcg gcctttaccc
     3781 caccacagcc atagtgcccg caaacgatga catggttcac cttcaggtga ttgacggcaa
     3841 aatcgagcac gctgagcaga ttgacatccg tatggatgac aagattggcg atattgcgat
     3901 ggacaaagat ctcccccggc tgcgtcccgg tgatcttgtc ggcaggcacg cggctgtcgc
     3961 tacagccgat ccacaggaat tcaggtgttt gtatatgagc aagccgttcg aaataggtcg
     4021 ggtcgtccgc cttgatctcc gaagaccagg ccttgttttc cagtaataac ttttctatcg
     4081 gcgtcattaa aataattttt tcgttacaaa gataagtgtt tgtcaggctg ctttgttccg
     4141 cacttcagcc gggccattct tccggac
//
LOCUS       DATGFG010000194         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_995518, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000194 DATGFG010000000
VERSION     DATGFG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995518"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VKQ52_03585"
     CDS             complement(<1..561)
                     /locus_tag="VKQ52_03585"
                     /inference="COORDINATES: protein motif:HMM:NF026047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ86293.1"
                     /translation="MSIAPATDVRKPAAATDFLPLQGTDYVEFYVGNAKQAAHYYKTA
                     FGFQSLAYSGPETGSKDKVSYVVRQNKLTFVFTTALRPDHPIAEHTHLHGDGVKVLAL
                     RVDDAAGAWRETTARGGKSYMEPTTIADKWGDVTLSGIHTYGDTVHLFIERKEYRGAF
                     MPGFREWRSQYNPPSTGLLYVDHCVGN"
     gene            992..>2081
                     /locus_tag="VKQ52_03590"
     CDS             992..>2081
                     /locus_tag="VKQ52_03590"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HLZ86294.1"
                     /translation="MLKVGILGGGQLGRMLLQAAANYPVETYVMENDENCPAAHLCHR
                     FIKGDIRNFDDVYRFGQGLNAVTIEIESVNEEALERLEAEGVKIYPKPSALRTIKNKI
                     TQKQYYTEHSVPTAEYIITQNLDGVRARMDLLPAVHKIGKGGYDGKGVQMIRTKDDVE
                     KGFDQPGVLEKMINVDKEIAQIIAISEKGETAIYPPVDMVFDQRLNLLDYQISPADLQ
                     QGLLWKVEAISLRAVKELKSPGIFAVELLVTKQGDVYVNETAPRVHNSGHHTIEANYS
                     SQFDMLWRVILGYPLGNTEAILPGAIVNLIGADGCEGEVHYEGLDEVLQMDNVFVHIY
                     GKNETKAGRKMGHVTIISREKQDLIYKAH"
BASE COUNT          514 a          419 c          663 g          485 t
ORIGIN      
        1 attaccgacg cagtggtcga catagagcaa gccggtggaa ggcggattgt actggctgcg
       61 ccattcccgg aagccgggca tgaaggcgcc gcggtactcc tttcgttcga taaagagatg
      121 gacggtgtcc ccgtaggtgt ggatgccgct tagcgtgaca tcgccccatt tatcagcgat
      181 ggtcgtgggt tccatatagc ttttaccgcc acgggcagtg gtctcgcgcc aggcaccggc
      241 agcatcatct acgcgcagcg ccaggacctt tacgccgtct ccgtggagat gagtgtgttc
      301 cgcgatagga tggtcgggcc ggagggctgt cgtaaagaca aatgtgagtt tgttctggcg
      361 gacaacatag ctcaccttat ccttgctgcc cgtttcgggt ccgctataag cgagggactg
      421 gaatccgaaa gcggttttat aatagtgggc ggcttgtttg gcattgccta catagaattc
      481 aacgtagtcg gtgccctgga gggggaggaa atccgttgcg gcagccggct tgcgaacgtc
      541 tgtagctggg gcgatagaca tagtaaaaac gtattttgat atttataaaa tggggataag
      601 gtcctgattc ggatctggag tagagaatgg gggtttgaga cgatcagggg cggatgaggg
      661 taagcgcctc catcaggaaa aagagatgca accgcttgtc gtcgaaaatt ttatgcgagt
      721 agtcagggcg catgaatatt gcatctaaag gtagaatata ttttgacatt tatcaacggg
      781 gttggataaa gttgaatttc ccccggatga gatggcctct ggataggatt agttttcagg
      841 caaaagggtg ttttgctctt gccgatggag tgttacaggg cccaaaaaac cgtatttttc
      901 cggtaggaat cgagtgtttt taccatatgg aagtgggttt tcggaattgg gaatcgagtt
      961 ttgtctatct tcgggggtaa actgttggat catgttaaaa gtaggtatct taggcggcgg
     1021 ccaattagga aggatgttgt tgcaggcagc ggccaactac cctgttgaga cctatgtcat
     1081 ggaaaacgat gagaattgcc cggcggcgca tctttgccac cggttcataa agggcgatat
     1141 ccggaatttt gatgatgttt accgttttgg gcagggcctg aacgcggtga ctatcgagat
     1201 cgaatcggtg aatgaagagg cgctcgagcg cctggaggcc gagggggtga agatataccc
     1261 gaagccatcg gcccttcgga cgataaagaa taagatcact caaaagcagt attatacgga
     1321 gcacagtgtt ccgacggcgg agtacattat cacgcagaac ctggatgggg tgagggcaag
     1381 aatggacctg cttccggcgg tgcataagat cggaaagggt ggttatgacg gcaagggtgt
     1441 gcagatgatc cggacgaagg atgatgtaga gaaaggtttt gatcaaccgg gggtattgga
     1501 gaagatgatc aatgtcgata aggagatcgc gcagatcatc gcgatcagcg agaagggtga
     1561 gacagccatc tatccgcccg tggatatggt ctttgaccag cgattgaatt tactggatta
     1621 ccagataagc ccggcggacc tgcagcaggg ccttctgtgg aaggtggaag cgatctcgct
     1681 gcgggccgta aaggaactga agagcccggg gatctttgca gtggaactgc tggtgaccaa
     1741 gcaaggtgat gtctatgtaa atgagacggc gccgcgggtc cataatagcg gacatcatac
     1801 gatcgaggca aattacagtt cgcagttcga tatgctctgg cgggtgatcc tgggttatcc
     1861 gcttgggaat accgaggcga tcctgcccgg ggcgatcgta aacctcatcg gggcagacgg
     1921 atgtgagggc gaggtgcact atgaaggact cgatgaggtg ttacagatgg ataatgtatt
     1981 tgtgcatatt tatgggaaga acgagacaaa ggccgggcgg aagatgggtc atgtgacgat
     2041 catcagccgg gagaagcagg accttattta taaggcgcat a
//
LOCUS       DATGFG010000195         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1450463, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000195 DATGFG010000000
VERSION     DATGFG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450463"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VKQ52_03595"
     CDS             <1..839
                     /locus_tag="VKQ52_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86295.1"
                     /translation="LEEQLGELSPRSRRKNTRVLRPLAIGASILALLLAGIFIGLSLN
                     RQNLGFQQKIADKNPVKNSVQPIVHPSTQQLPAPAANTAPTTPPANKPKDSVPNIINS
                     RVAAIVPPVTKPPRTVTPKERPGAPKKGAILPPVTKDSAAGMNPNTTATVAQAVHRPD
                     VVETKSENKSDKETAKTSIASQVSVGANGYTVGTFGGINDLQLTVTNHSAYPLDLVVV
                     EIQYIQANKKVYKTENLYFRNIGAGSALMQEAPRTSRGIKVRYKITTINSKDLGLSYS
                     GI"
     gene            1039..1356
                     /locus_tag="VKQ52_03600"
     CDS             1039..1356
                     /locus_tag="VKQ52_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86296.1"
                     /translation="MRNAMVQLCLVIIGVSSIHAVKLLQTSSFHVRVYPADGADRVWA
                     VQGKDSIEMIDVNGEYFLRSINPGHWHLSVEANEPYQNTRFNVSDVRPGTDVDLGEIH
                     LQK"
     gene            complement(1405..>2304)
                     /locus_tag="VKQ52_03605"
     CDS             complement(1405..>2304)
                     /locus_tag="VKQ52_03605"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HLZ86297.1"
                     /translation="IWLTGADPGQVLPTGWAGRYLDQQYPHFPQDYPNAGMPDPLAIQ
                     VGSLVSPALQGPSLTMGMAISNPNSFYDLIGDKTDTTPNTRAGDQLAYIREMAVKTDQ
                     YAGVIKKAAQKVTKQSDKYPAAGKNPLADQLKIVARLVAGGLKTQIYLVSMGGFDTHA
                     KQTDNADTTTGAHARLLARLSEAINAFQDDLSVLQVGNRVMGMTFSEFGRRIQSNASG
                     GTDHGAAAPVFVFGEGVKPGIVGRNPVFPDHLTVNDNVMMQTDFRAIYSTLLEKWFQA
                     DTRETAAVLLKDYAVLPLVGENT"
BASE COUNT          541 a          731 c          564 g          468 t
ORIGIN      
        1 cactggaaga gcaacttggg gagctttcgc cacgctcccg gcggaagaac acccgcgtcc
       61 tccggccgct cgccatcggc gcctccatcc tggccctgct cctcgccggc atcttcatcg
      121 gcctctccct caacaggcaa aacctgggct ttcagcaaaa gatcgctgac aagaaccccg
      181 tcaaaaatag tgtgcagcct atcgtgcacc cttccacgca gcaactgccg gcgcctgcgg
      241 cgaacaccgc cccgaccacc ccgcccgcca ataaaccaaa ggactccgtc ccgaacatca
      301 tcaacagccg ggtggccgcc atagttcctc ccgtcacaaa gccccctcgc acggtgaccc
      361 cgaaggaacg accgggcgca ccaaaaaaag gcgcgatact cccccccgtt acaaaggatt
      421 ctgccgccgg catgaatccg aatacaaccg caacggttgc tcaggccgtt caccggccgg
      481 atgtggtgga aacaaagtcg gagaacaaat cggacaaaga aaccgctaaa acgtcgatag
      541 ccagccaggt ctccgtcgga gccaatggct ataccgtagg aaccttcggc ggcatcaacg
      601 acctgcaact caccgttact aaccattccg cctatcccct cgacctggta gtagtcgaaa
      661 tccaatatat ccaggcaaat aaaaaggttt acaaaacgga gaatctgtat ttccgcaaca
      721 tcggcgcagg ctctgccctg atgcaggaag cacccaggac ctcgcgtggg atcaaggtcc
      781 ggtacaagat caccaccatc aactcaaaag atctgggcct gtcctactcg ggaatataag
      841 catctcatta acaaaaaggt aaacgaagtt ttcccctatt cttcttcatt tggcacagga
      901 attgacatca gtgtgtccga ttagcctctg ccactctgtt caatctctta attagaagcc
      961 tgtcataaca aaaattgtgc atttgtcgat aaatgcgtaa ccactaatca aagcattttt
     1021 taaaaatttg gaaccggtat gagaaacgcg atggtgcaat tatgtctggt tattataggt
     1081 gtctctagta ttcatgctgt caaattatta caaacttcgt ccttccacgt tcgggtgtac
     1141 ccggcagacg gggcagacag ggtctgggcc gtccagggaa aagactcgat agaaatgatc
     1201 gatgtgaatg gggaatactt cctgcgctcg ataaaccccg gacactggca tttatccgtg
     1261 gaagctaacg agccttacca gaacacccgc ttcaacgtga gcgacgtaag accgggcacg
     1321 gacgtcgatc tcggcgagat ccacctccag aaatagaccc ccggcccctc ctcttcccgg
     1381 ccggccatcc acagggccgg gcggtcaggt gttctctccc accaggggca gcacggcgta
     1441 atccttcaac agcaccgcgg ccgtttcccg ggtgtccgcc tggaaccatt tctccagcag
     1501 ggtactgtaa atggccctga aatcggtttg catcatcaca ttatcattga cagtcaagtg
     1561 atcggggaaa acggggttcc gcccgacgat ccccggtttc accccctctc caaagacaaa
     1621 cacgggcgcc gccgccccat ggtccgtccc accgctggca ttcgactgga tccgcctgcc
     1681 aaattcggag aaagtcatcc ccatcacccg gttgcccacc tgcaaaaccg acaggtcatc
     1741 ctggaaggca ttgatcgctt ccgatagccg ggccagtaat ctggcatgtg cgcccgtcgt
     1801 cgtgtccgcg ttatccgttt gtttcgcatg tgtatcgaac ccgcccatgc tcaccaggta
     1861 gatctgtgtc ttcaaccctc ccgcgaccag ccgggcgacg atcttcagct gatccgccag
     1921 cggattcttc cccgccgcgg gatacttatc ggattgcttc gtgaccttct gggcggcttt
     1981 cttgatcaca cccgcgtact gatccgtctt gaccgccatc tcccggatat aagccaactg
     2041 atcccctgcc cgcgtattgg gcgtcgtgtc cgtcttatcg ccgatcagat cgtaaaaact
     2101 attgggattg ctgatggcca tgcccattgt cagcgagggg ccctgcaagg cgggtgagac
     2161 aagggagccc acctggatcg ccaggggatc gggcatcccc gcattgggat aatcctgggg
     2221 gaaatgcgga tactgctggt caagataccg ccccgcccag cccgtaggca acacctgtcc
     2281 cggatcggcg cccgtcagcc agat
//
LOCUS       DATGFG010000196         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1566961, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000196 DATGFG010000000
VERSION     DATGFG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566961"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VKQ52_03610"
     CDS             <1..526
                     /locus_tag="VKQ52_03610"
                     /inference="COORDINATES: protein motif:HMM:NF042600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6770 family protein"
                     /protein_id="HLZ86298.1"
                     /translation="LFLVGEGYRREANAAGIALTVLSMGRNNVIGNTKMVVTDMVIMQ
                     FDEKYKITGAAIYDKKNNTAMVSTAVDYNSQHYIAKFLKAVGAFDYEFTTGDEDHSNF
                     AVCYSDYERSSEYHGQTFNAIRYNGTKFSTDKIELKTKASRMRVFPAKAGSVMIMEYF
                     KKDKRLDFRLEKLG"
     gene            complement(570..989)
                     /locus_tag="VKQ52_03615"
     CDS             complement(570..989)
                     /locus_tag="VKQ52_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86299.1"
                     /translation="MAKYDNNPSVGSIGNATAQTNSSALTARGICSKIFLHDHSPVYP
                     QRFARRSDISLSLSTSIYQELVANHWLDNRNYLTAPGDTISVRIQANPSAYPVTSALT
                     APQFQFYTDELDCMYAAHKFYSDYDKTTIHFLGSPCQ"
     gene            complement(1074..1802)
                     /locus_tag="VKQ52_03620"
     CDS             complement(1074..1802)
                     /locus_tag="VKQ52_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86300.1"
                     /translation="MKNSKLYALLGFALLAGCSKKSSTTTPGTTGTTDSATLTVINGY
                     GTGKYKVGDTVNIWSQAIPANMVFDTWTGYGALLQNAGEWHNSLVMPAQDVTLTASQK
                     SLAPFTLNYEKIKGVNILKNVYYYFPSAHKGIVYLLHGTGGTAQTLVNQFEWTQMISD
                     LVSADYAIVVTEAEEVSLNKDLNGDGYIRWVLSPLDTTTNVDYGNFRALIDTFRARGY
                     TNSSIPQYSIGMSDGGAFSAALSF"
     gene            complement(1827..>2263)
                     /locus_tag="VKQ52_03625"
     CDS             complement(1827..>2263)
                     /locus_tag="VKQ52_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ86301.1"
                     /translation="GMHFSKIGYVPAAGNSSTQRDYTFTDPSLTNDSNFYQLKEITLD
                     SHATYSNVVLIRDPDATPSLTVLPNPFTTGLELVFSHVPTGPVQIRLLDITGRELLRQ
                     AGTQSSGARLHLDFSGSSLTAGVYLLEVRSATGTQIQRVIKK"
BASE COUNT          489 a          469 c          752 g          553 t
ORIGIN      
        1 gctgttcctg gtgggcgagg ggtatcggcg ggaggcgaat gccgcgggta ttgcgctgac
       61 ggtgttgtcc atggggcgta ataatgtcat cggcaatacg aagatggtgg tgacggatat
      121 ggtgatcatg cagttcgatg agaagtacaa gatcaccggt gcggctatct atgacaagaa
      181 gaataatacg gcgatggtga gcacggccgt cgactacaac agccagcatt atatcgcgaa
      241 attcctaaag gcggtggggg cttttgatta tgagtttacc accggtgacg aggatcattc
      301 caatttcgcg gtttgttaca gtgattatga gcggagttcg gagtatcatg ggcagacctt
      361 caatgcgatc aggtataacg ggacgaagtt ttccactgat aagatcgagc tgaagacgaa
      421 ggcgagccgg atgcgggtgt tcccggcgaa ggcgggatcg gtgatgatca tggagtattt
      481 caagaaggat aagcggctgg atttcaggtt ggagaagttg gggtaggggg aatgggataa
      541 aaggccacct ttcaggtggc cttttatcgt tattggcagg gagatccgag gaaatggatc
      601 gtcgttttgt cgtagtcgct gtagaacttg tgtgcggcgt acatgcaatc gagctcatcg
      661 gtatagaatt ggaattgagg ggctgtcaat gccgaagtaa ccgggtaagc ggaggggttt
      721 gcctgaatcc tgaccgaaat ggtatcgccg ggtgcggtta aataattcct gttgtcaagc
      781 cagtgattgg ctactagctc ctggtatatc gaggtggaga gagacaggct gatatcgctt
      841 cgccgggcaa aacgctgagg ataaaccggg ctgtgatcat gcaggaatat tttactgcaa
      901 atcccccggg cggtcagggc gctggaattg gtctgcgccg tggcgttccc gatggaaccc
      961 acggatgggt tgttgtcata tttggccatg caaaactgga aaggtgtcgt ggaggtgttg
     1021 gttacctgtg tgccggtagg ggcgcaatag gagacgccgg ccgcatattt atattaaaac
     1081 gagagagcgg ccgagaaggc tcccccgtca ctcatgccga tggaatactg cgggatggag
     1141 ctgttcgtat aaccccgtgc gcggaaagtg tcgatcaggg ccctgaagtt gccgtaatct
     1201 acattggttg ttgtatccag cggggacaga acccagcgga tatagccatc accgttgagg
     1261 tctttgttga gggatacctc ttcggcttca gtaacgacga tagcataatc cgccgacacc
     1321 agatcactga tcatttgggt ccattcgaat tggttcacca gggtctgggc ggtcccaccc
     1381 gtaccatgca ggagatagac gatcccttta tgggccgaag ggaaatagta atatacattt
     1441 ttcaggatat tgaccccttt gatcttttca tagttgaggg tgaagggagc taaggatttt
     1501 tgggaggcgg tgagcgtcac atcctgtgcg ggcatcacca ggctgttatg ccattcaccg
     1561 gcattttgca acagggcgcc ataacctgtc caggtgtcga agaccatatt ggccgggatg
     1621 gcctgggacc aaatgttcac ggtatcaccg accttgtatt tgccagttcc gtagccattg
     1681 atcacggtaa gggttgcgct gtccgttgtg ccggttgtgc cgggtgtggt ggtgctgctt
     1741 tttttggagc atccggccaa tagggcgaag cctaataaag cgtacagttt tgaatttttc
     1801 atattaattg gacttaaggg ttgtatctat ttctttatca cgcgttggat ctgggtgccc
     1861 gtggcggagc ggacctcgag gaggtagacg ccggcggtga gggatgaccc ggagaagtcg
     1921 aggtggaggc gggctccgct ggactgggtg cctgcctggc gcaggagttc gcggccggtg
     1981 atgtcgagca gcctgatctg cacggggcct gtgggaacgt ggctgaagac gagttcgaga
     2041 ccggtggtga aggggttggg cagtacggtg aggctgggcg tagcgtccgg gtcgcgtatc
     2101 agcacgacgt tgctataggt ggcgtggctg tcgagggtga tctctttgag ctgatagaag
     2161 ttactatcgt tggtgaggga cgggtcggtg aaggtatagt cccgttgggt gctgctgttg
     2221 ccggcggcgg gtacatagcc gatcttcgag aagtgcatgc cgt
//
LOCUS       DATGFG010000197         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1574120, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000197 DATGFG010000000
VERSION     DATGFG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574120"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VKQ52_03630"
     CDS             complement(<1..320)
                     /locus_tag="VKQ52_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86302.1"
                     /translation="MSKYEEIKQRFLGRGIKEENIDYAIESVRYGTKREHILENLTAD
                     YRGMNREEAVPLVEALFIANGGEFRKENRGGYLYGGALLAAGLLLAFYIGYVLLFGGI
                     LFRP"
     gene            complement(349..>2387)
                     /locus_tag="VKQ52_03635"
     CDS             complement(349..>2387)
                     /locus_tag="VKQ52_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLZ86303.1"
                     /translation="DSKYIAFVDNKVRIKVVEIATGKITQADVGGVNLERGGMGLVWS
                     PDSKWLAYSKTGPNNFRRIMIWSLGSGKATAFSDGLADATQPAWDRDGRHLYFLASTD
                     AALGSGWANTSAILARPGYAAYVTVLRKEDGPPFPLKSDDEADSAARGGSSGGAKKDT
                     LAKKDTAANDRGVRIDFENLDRRTVALPIATANYRQLISGPKGTVFIITGDGTLSRFT
                     LDTKKLDEYAQHVGQADVSANGEKLLIQSGNSWRVVSAAKPPAPGEGAVAVNLRMDLD
                     RIEEWKQIFTEAWRYERDYFYDPALHGRDWQEVWAYYSPMIPYIRHRSDLTYLLDQLG
                     GELSVGHSFVFGGDFPAVDTLRVGLLGADLRVNEGRWQIKRIYTAESWNPDLKAPLAQ
                     PGLKVSEGDYLLAIDGVALGSDVDPYSLLDGKAGQQTMLLVNNKPATEGAWSIRVEPV
                     PNENALRQRAWVEDNRRKVDELSGGRLGYVWVPNTGGPGFVSFNRYFFAQQDKQGAVI
                     DERFNGGGSLDDYMVDLMIRRQRAAITNEVPDGVAFRLPAGILGPKVLLINELAGSGG
                     DFFPWVFRHQKVGPLIGTRTWGGLVKSSVHYALVDGGAITAPDNAVFDPEAHQWIAEN
                     QGVPPDIEVKTEAAAVAKGKDPQLERAVQECLRLLEKEPRGSLEHPPYSKPARP"
BASE COUNT          489 a          871 c          537 g          490 t
ORIGIN      
        1 atcggccgga acaggatccc gccaaacaac aacacatagc caatataaaa agccaataat
       61 aaacccgcgg caagaagcgc gccgccatac agataaccgc ctctattctc cttcctgaac
      121 tcaccgccat tggcgataaa caaggcttcc acgaggggaa cggcctcttc gcggttcatc
      181 ccgcggtaat ccgcggtcag gttctcgagg atatgctccc gtttcgtccc gtaccggacg
      241 gactctatgg cataatcgat attttcttcc ttgatccccc tgccaaggaa tcgctgtttg
      301 atttcctcat acttactcat gactatagtg tttaggtttg tgtcgaactc atggcctcgc
      361 cggcttcgaa tacggcggat gctccagtga tcctcgcggc tccttctcca gcaaccgcag
      421 acactcctgc accgcccgct ccagctgtgg atccttcccc tttgccaccg ctgccgcctc
      481 cgtcttcacc tcgatatccg gcggcacccc ctggttctcg gcgatccatt gatgcgcctc
      541 cggatcgaaa acagcattat ccggcgccgt tatcgcccca ccatccacca gcgcataatg
      601 caccgacgac ttcaccaacc cgccccaggt acgcgtcccg atcaacggtc ccaccttctg
      661 atgccggaac acccagggga agaaatcacc ccccgatccg gccagttcat ttatcagcaa
      721 caccttcggc ccaaggatcc ccgccggcaa ccggaatgcc accccatccg gaacctcatt
      781 cgttatcgcc gcccgctgcc gtcggatcat caggtcgacc atataatcat ccagcgatcc
      841 gccgccattg aaccgctcgt cgatcaccgc cccctgcttg tcctgttgag cgaaaaaata
      901 ccggttgaac gaaacaaacc ccggtccgcc ggtattgggc acccacacat agccgagcct
      961 gcctcccgac aactcatcca ccttccggcg gttatcctct acccaggccc gctgccgcaa
     1021 cgcattctca ttcggcaccg gttctacccg gatactccag gccccctccg tcgcgggttt
     1081 attgttcacc agcaacatcg tctgctgacc ggcctttccg tcgaggagcg aatagggatc
     1141 gacatcactc ccaagcgcca cgccatcgat cgccaacaga taatcccctt ccgaaacctt
     1201 caacccgggc tgcgccaggg gagctttcag gtcggggttc cagctctcgg ccgtgtagat
     1261 ccgtttgatc tgccagcgtc cctcgttaac gcgaaggtcc gcgcccagca aacccacccg
     1321 caatgtgtcc accgcgggga aatcaccgcc gaatacaaag ctatgtccca cgctgagctc
     1381 cccgccaagc tgatcgagca gataggtcag atcgctccgg tgccggatat acgggatcat
     1441 cggcgaataa tacgcccata cttcctgcca gtcgcggcca tgcaacgccg gatcatagaa
     1501 atagtcccgc tcataccgcc acgcctcggt aaagatctgc ttccactctt cgatccggtc
     1561 gagatccatc cgcaggttta ccgccaccgc accttctccc ggcgcgggcg gcttcgccgc
     1621 agacaccacc cgccagctat tacccgactg gatcagcagc ttctcgccat tggcggacac
     1681 atccgcctgc ccgacgtgtt gtgcatattc atccagtttc ttcgtgtcca gggtaaacct
     1741 gcttagcgtg ccatcgccgg tgataataaa caccgtcccc ttcggcccgc tgatcagctg
     1801 ccggtaattg gccgtcgcga tgggcaacgc caccgtccgc cggtcaaggt tctcaaaatc
     1861 gatccgcacc ccgcgatcat ttgccgcagt atcttttttt gccagagtat ctttcttcgc
     1921 gccccccgaa cttccaccgc gagccgcact atccgcttca tcatcgctct tcaacggaaa
     1981 aggcggcccg tcctccttcc gcaacaccgt cacatacgcc gcataacccg gcctcgcgag
     2041 gatcgcactc gtattggccc atccggaccc aagcgcagca tccgtgctgg ccagaaaata
     2101 caaatgccgc ccatcccggt cccaggccgg ctgcgtcgcg tctgcgagcc catcgctgaa
     2161 agctgtggcc ttccccgatc ccagcgacca gatcatgatc cgccggaaat tattcggccc
     2221 cgtcttcgaa tatgccagcc actttgaatc cggtgaccat accagcccca tcccgccccg
     2281 ctcgaggttg accccgccga catcggcctg cgtgatcttc cccgtcgcga tctcgaccac
     2341 ttttatccgg actttattat cgacaaaagc gatgtactta ctatccg
//
LOCUS       DATGFG010000198         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1104445, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000198 DATGFG010000000
VERSION     DATGFG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104445"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VKQ52_03640"
     CDS             <1..603
                     /locus_tag="VKQ52_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLZ86304.1"
                     /translation="KFSDIKILLATHAHFDHVGAMAAIRQTTGARLMIHERDVPVMAD
                     GGNSDFDFGGHGPMFQPVKVDRLLHDGDIVKLGGMEILVLHHPGHTKGACSFLFTVKD
                     ETRAYRVLIVNMPSILSETKFPGMPTYPEVGKDYAFTLDTMPKLQFDIWLSSHASQFD
                     LQKKHKPGDGYNPAAFFDRPKYESSVEALQKAYLKRINDK"
     gene            678..1394
                     /locus_tag="VKQ52_03645"
     CDS             678..1394
                     /locus_tag="VKQ52_03645"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLZ86305.1"
                     /translation="MQPQNYHCTITAPITAAEAFEKISRVGDWWAVNFKGNAKNLYDR
                     FTVHFARTTWSIMEIVEVVPDQRIVWKVVDCYLPIFQDPYLWKNNHIAWEISTEGAQT
                     RVTMTHIGLVPGVECYEDCRKGWNFYIGESLLGLLIAGKGLPGSGIFADIFVGSRKYE
                     GLMFSKTEPFPDNADGYIIVDIRENSGERVLSAYAVDTLDIKGMSPQQLKGDYYMVLE
                     NRPVSGNIQPLEDLRQIIPA"
     gene            complement(1425..1841)
                     /locus_tag="VKQ52_03650"
     CDS             complement(1425..1841)
                     /locus_tag="VKQ52_03650"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLZ86306.1"
                     /translation="MSEIRVMTDAFAATIYEKDLEGFLSLFDSGVNVFDMWEQWSYEG
                     LAAWREMVKGWFAGLGAERDVVSFEEVQIQEAGDMGFLTAIVRFAAVSAEGKELRFLQ
                     ERLTWVLRRKDGGWKIVHQHTSVPLDGGTMKGILQR"
     gene            2164..2487
                     /locus_tag="VKQ52_03655"
     CDS             2164..2487
                     /locus_tag="VKQ52_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HLZ86307.1"
                     /translation="MNKYERLKADLTAAGTGKMKAFGNSMLPILKSGSHLTFTQTPDY
                     QVGDIVFCKVRGRYIDAHKIVKTDPNKGFLIANNHGFENGWTKIIYGKVIVAEYQGRV
                     IYTAG"
     gene            complement(2530..3099)
                     /locus_tag="VKQ52_03660"
     CDS             complement(2530..3099)
                     /locus_tag="VKQ52_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86308.1"
                     /translation="MKKLIFGCGAGIFFFFSPAVLSSVHAQSGCSGVYLSADDLAAGR
                     LISCVKPHRSDNVMNSKYFFIQQNGAVKKLGRKDLYAVKYCDGRVFRVFGDGFFAWLN
                     PGEAIPLYEVVENPVAKGNICRTEYYFSKDARSAIQQLTRDNVKAAFAGDAKFEEALD
                     LQFGSDRDLYAYDSYNKCYRLDRVYAICK"
BASE COUNT          897 a         1027 c          767 g          713 t
ORIGIN      
        1 aaattctccg atatcaaaat actcctcgct acccatgctc atttcgatca tgtcggcgcg
       61 atggcggcaa taaggcaaac gacgggcgcc cggctcatga tccacgaaag agatgtgcca
      121 gtaatggcag acggcggcaa ctcggatttc gactttggcg gccacggtcc catgttccaa
      181 ccggtaaagg tggaccggct gctgcacgac ggggatatag taaaactggg cggcatggag
      241 atcctggtcc tccaccaccc cgggcacacc aagggagcct gtagctttct ctttacggta
      301 aaagacgaga cccgagctta tcgtgtcctg atcgtgaata tgccctccat tctcagcgaa
      361 acaaaattcc ccggcatgcc cacctatccc gaggtgggaa aggactatgc ctttaccctg
      421 gatacgatgc caaaattaca attcgatatc tggctttctt cccacgccag ccagttcgat
      481 ctccaaaaaa agcacaagcc cggagacggc tacaacccgg cagccttctt cgaccggcca
      541 aaatatgagt cgtcggtcga agccctgcaa aaggcgtatt tgaaacggat aaatgataaa
      601 taggcgcgaa aattctccat tttcaataaa cccttttccg ccccggccgg atagctttgc
      661 tgtataaaca caaacttatg caaccgcaaa actaccactg caccatcacc gcccccatca
      721 ccgcggcaga ggcttttgaa aagatcagcc gggtagggga ctggtgggcc gtaaatttca
      781 agggaaacgc caaaaacctc tacgaccgct ttacggtaca tttcgccaga acgacctggt
      841 cgatcatgga gatcgtcgag gttgtcccgg atcagcggat cgtatggaaa gtggtagatt
      901 gctatcttcc catattccag gacccctatc tatggaagaa taatcacatc gcctgggaga
      961 tctcaacaga aggagcccag accagggtaa cgatgaccca tatcggcttg gtccccggtg
     1021 tagaatgcta tgaagattgc cggaagggct ggaattttta tatcggcgaa agtcttctcg
     1081 gactattgat cgcgggcaaa ggactcccgg gcagcggcat attcgccgat atctttgtcg
     1141 gcagcagaaa atatgaaggg ctgatgtttt ccaaaacgga gccgttcccc gacaacgccg
     1201 acggctatat tatcgttgac atccgggaaa atagcggcga gcgggttctc tcggcctatg
     1261 cggtcgatac cctggatata aaaggcatga gcccccagca actaaagggg gactattaca
     1321 tggtcctcga aaaccggccg gtatccggga acattcaacc gctggaggac ctccggcaga
     1381 tcatcccggc gtaaatcgac ccgcaccggc tcaattcgat ggcctcaccg ctgcaagatc
     1441 cccttcatcg tccccccatc caacggcacc gacgtatgct gatgcacgat cttccacccg
     1501 ccatccttcc tccgcagcac ccatgtcaac cgctcttgca agaaccgtaa ctcctttccc
     1561 tcggccgaga ccgccgcaaa acgcacgatc gccgtcagga atcccatatc tccggcctcc
     1621 tgtatctgca cctcctcgaa gctgaccaca tcccgctcgg ccccgagccc ggcaaaccat
     1681 cccttcacca tctcgcgcca ggcggccagc ccctcatatg accattgttc ccacatatcg
     1741 aaaacattga ccccggagtc gaaaagcgaa aggaacccct cgaggtcctt ttcataaata
     1801 gttgcggcaa atgcatcggt cattaccctg atctcgctca taatattggt ttaattccct
     1861 gaaaataaag aaaagaagaa agatttttca aactatgcaa ataggttgca cagatgcgcc
     1921 cccatctttg tgcggcaatc agccagtccc tgtagcttaa aaggaaaagt ctgcggcaaa
     1981 attggcttac cgtagcgtgt atggagtatc ccgtaatgtc cacgcgaaat tatgggtaga
     2041 gtttacagtg caccgaaaag cgtaatggcc gagtagggaa gcgtatttac agccatactg
     2101 cccggcaacg ggaatgtgag ttcgaatctc accagggaaa cgataatcct aaactataag
     2161 aaaatgaaca agtacgaacg gctaaaagcg gacctcaccg cagccggcac aggaaaaatg
     2221 aaggccttcg ggaattccat gctacccatc ctaaaaagcg gaagccatct gacattcaca
     2281 cagactcccg actaccaggt cggcgacata gtcttttgca aggtccgggg tcgatacatc
     2341 gacgcccaca agatagtaaa aacagacccg aacaaagggt ttctcattgc caataaccac
     2401 ggcttcgaga acggctggac aaagatcatc tatggaaaag tgatcgtcgc ggaataccag
     2461 ggccgggtca tctacaccgc cggatagccg gatagcacat ccccgccgtt catttacaag
     2521 ccgcccccct cacttacaaa tagcatagac ccgatccagc ctataacatt tgttatagct
     2581 atcatacgca tacagatcgc gatcactccc aaactgcaga tcgagcgcct cctcgaattt
     2641 cgcatccccg gcaaaagccg ccttcacatt atcccgcgta agctgctgga tcgccgacct
     2701 cgcgtcctta ctgaaataat attccgtccg gcagatattc cccttggcca ccgggttctc
     2761 gaccacttca tataagggaa tggcttcgcc gggattcaac caggcaaaaa atccgtcccc
     2821 gaatacccgg aatacccggc cgtcgcagta tttcaccgca tacaggtcct tccgccccag
     2881 ctttttcacc gcgccattct gctggataaa gaagtatttg ctattcatga cattgtcgga
     2941 tcggtgtggt ttgacgcagc tgatcagtct tccggcggca agatcatcgg cggataaata
     3001 taccccgctg caaccggact gcgcatggac cgaagagaga acggccggag aaaagaagaa
     3061 aaagatcccg gccccgcaac caaagattag ctttttcata tgcaatcgta tttagcttaa
     3121 gttactcctt ttccgccgga cacccatcgc gagcgcaccc ctttaacgga gcaataacgc
     3181 gccgccaacg atcgcaaacc tttcgccaac cgcagcctcc tccaccgatc gccaacatca
     3241 cgccacccgt cgccaccgat cccgtccgcc cttacttcgc cttccggata tacacactcc
     3301 caaacgtcgt cttcatcata acctccggtc cgcccccatt gatcttcccc aacacccatt
     3361 cgtcgatatt gatcttatac atatggccct ccgtattcct gctc
//
LOCUS       DATGFG010000199         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1450109, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000199 DATGFG010000000
VERSION     DATGFG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450109"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VKQ52_03665"
     CDS             <1..1114
                     /locus_tag="VKQ52_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86309.1"
                     /translation="QTYNYGSTNTTGLYPNAAPNPSLNWEYTATANVGVDFGFLQNRL
                     TGSIDLYHSYTKDMIMPVTLPASSGIPSQILTNIGKSENKGIEIHLAGDIIQAHSNHA
                     FSWSADVNFYLNRGEITQLLPSINTTLDGKPADIANKWFVGRPIGSFYDYVRAGIWQA
                     TVYDSAQAKGLGQTLTGNKSVIGQAKVLDRNHNGVIDAGDEAIVGTPQPKWEAGTTQR
                     FAYRGFDLTVVAFARVGGTIESALFGAGFASTMQGNYNNVNVAYWTPKNPTNRWPEAN
                     SAQTNPPYHSTFAYFDGTFLKIRSMTLGYTLPPAAVKTVGLKSIRFYATAKDPFILFS
                     AYRNKYHGIDPELTGTTDNLNTPATWSMLFGVNVGF"
     gene            1153..>2042
                     /locus_tag="VKQ52_03670"
     CDS             1153..>2042
                     /locus_tag="VKQ52_03670"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86310.1"
                     /translation="MKKQYILMALSAGLLLTLAPACNKQLNEHPNSVVTPDFFSTAQG
                     FQSGLDAAYGGMRNLWGTENLFTITCIGTDEFITGNDGTGNNINYYSSGYTPADSKLS
                     GIWNPCYTFINDCNGLVDYGPKIPNLDSVKRLEMMAEAKFLRANYYFVLVQFWGPVTL
                     NKHFQATPTTAAQRNPVSDVYDFIVQDLQDAIAGLPPSPTQGGVLPGKATAAAARHLL
                     AKVYLTRAGSSAAHSDDYQNAYNTASSLINSSAGLGLGLLPDFGSVYQEGNEGNKEVL
                     WTIQHTASLAYNGSPTQNSSG"
BASE COUNT          457 a          508 c          601 g          476 t
ORIGIN      
        1 gcagacgtat aattatggtt cgaccaatac gacggggctt tatcccaatg ccgcgccaaa
       61 tccatcattg aactgggaat atacggcaac ggcgaatgtg ggtgtggact tcgggttttt
      121 acagaaccgg ctgacaggat cgatcgatct gtatcattcc tacaccaagg atatgatcat
      181 gccggtgacc ttaccggctt cctccggtat tccctcccag atactcacca atatcgggaa
      241 gagcgagaac aaggggatcg agatccatct tgcgggggat atcatccagg cgcatagcaa
      301 ccatgctttc tcctggtcgg cggatgtgaa tttctatctc aaccggggag agatcactca
      361 actgctgccc agcatcaaca ctacgcttga cggcaagccg gccgatattg ccaacaagtg
      421 gttcgtgggg cggccgatcg gttccttcta tgactatgtg cgggcgggga tctggcaggc
      481 tacggtttac gactccgcac aggccaaggg gctggggcag acgctcaccg ggaataaatc
      541 ggttatcggg caagccaagg tcctggaccg gaatcacaac ggggtgatcg atgcgggtga
      601 cgaggcgatt gtcggtacgc cgcagccgaa gtgggaagcc gggacgacgc agcggtttgc
      661 ctatcgcgga ttcgacctta cggtcgttgc ttttgcgcgg gtggggggaa ccattgaatc
      721 cgctttgttt ggagccggtt ttgccagtac gatgcagggt aactataata atgtcaacgt
      781 cgcctactgg acgccaaaga accccaccaa ccggtggccg gaagctaatt cggcacagac
      841 gaacccccct tatcattcca cttttgcgta tttcgatggt acttttctca agatcaggag
      901 tatgacgctt gggtatacgt tgccgccggc ggcggtgaag acggtcgggc tgaagtcgat
      961 ccggttttat gcgacggcga aggacccttt tattcttttc tctgcctacc ggaacaagta
     1021 tcatggcatc gatccggaat tgacgggtac gacggataat ctcaataccc cggccacctg
     1081 gtcgatgcta tttggggtga atgtcggttt ttaattggct gtacgccggt ataattttta
     1141 aatatgaaca atatgaagaa gcaatatatt cttatggcgt tgtctgcggg tttgctgctg
     1201 acccttgcgc ctgcctgtaa caagcagttg aatgaacacc cgaactctgt cgtcacgccg
     1261 gacttttttt ccactgcgca gggttttcaa tcggggctgg atgcggccta tggcgggatg
     1321 cggaatttgt gggggacgga gaatcttttt acgatcacct gtatcggaac ggacgaattc
     1381 atcacaggta atgacggaac ggggaataat atcaattatt acagcagcgg gtatacgccg
     1441 gccgacagca agctgtcggg tatctggaat ccctgctaca cttttatcaa cgactgtaac
     1501 gggcttgtcg attatggccc gaagatcccc aacctggatt cggtgaagcg gctggagatg
     1561 atggccgagg cgaagttcct gcgggccaac tattattttg tgctggtgca gttctggggc
     1621 ccggtgacgc tcaacaaaca tttccaggct acgccgacga cggcggcaca gcggaacccg
     1681 gtgtccgatg tatatgattt tatcgtacag gacctgcagg atgcgatcgc cgggttgccg
     1741 cccagcccga cgcagggcgg ggtgttgccg gggaaggcaa cggcggcggc tgcgcggcac
     1801 ctgctggcaa aagtgtattt aacacgcgcc ggttcttcgg ctgcgcattc ggatgactat
     1861 cagaatgcat acaatacggc ttcctccctg atcaacagca gcgccgggct tggacttggc
     1921 ctgttgccgg attttggaag tgtttaccag gaaggcaatg agggcaataa ggaagtgttg
     1981 tggacgatac agcatactgc gtcgctggcg tataatgggt cgcctacaca gaacagcagt
     2041 gg
//
LOCUS       DATGFG010000200         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_423237, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000200 DATGFG010000000
VERSION     DATGFG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423237"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..1097
                     /locus_tag="VKQ52_03675"
     CDS             426..1097
                     /locus_tag="VKQ52_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ86311.1"
                     /translation="MKVPVGIVDDKSQNLHLFAERLNYSDDIAIVLTAGSGDDFLLQM
                     KKLPPSAQPVVILMDIGMPGMDGIETVRVAKLLYPHINFLMLTVFDDDDKLFEAIKAG
                     ADGYLLKNDKLAAIIDCIVQLSETGSAPMSPRIARKTLDLLMKASLPEMENETQEKKD
                     YALSDRETEILKLTVDGYAYKAIAERLFLSSHTVRKHIANIYIKLHVSSKAQAIKIAT
                     KNKLI"
     gene            complement(1153..>2590)
                     /locus_tag="VKQ52_03680"
     CDS             complement(1153..>2590)
                     /locus_tag="VKQ52_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ86312.1"
                     /translation="YTRTIVEDGMHRIWVGTESGIFIYDRSGRLVRHLSRQAGLLNDI
                     IYSLLPANSSRNSFFASTNFGLSAIFPEGTIKNYPRELGLQENEFNTLSCARSPEGRL
                     FFGGINGITAFYPSELSLPRDSSKIAVVRFAVNDSAYTSFGNAWQGDTIRLPYNQDHL
                     QFDLAAIGLLNPSDYLYKYRLEGFDRSWQTTTQPTGIRYILPPGKYWLDINCSPVLYS
                     TRSLHKRIRIIISPPWWKTWWFVLLSAFATVLLIFGISYFILHQRYLWKLRKLEVDQQ
                     VVKERERISRELHDNIGTQLSHISNTIDWLLETPHAFNKEEEKDKLADINDTARSLVV
                     DLRETIWAMKKEFILLDEFADKLKLYLQSQTSLQPQMETIITEDIQKKYSFSPIEALN
                     VFRICQEAIANSVRHSQAERICLSIRSGRDVDFCFTIEDNGQGFVRQQQYYGHYGLEN
                     MTHRAAESGATLSLETEPGKGTRVTVSK"
BASE COUNT          635 a          694 c          659 g          602 t
ORIGIN      
        1 agggaatcgc tgcgggtgcc cttgcgcgcc gtcttattat agacgctggc attatacagc
       61 cgctcatggc tgacagcatc ggttatcatc ccgtgcaata tccggctctg cccgggcgca
      121 gaccggcaac aacataggat aaccagcaga gaaagaagaa attgccactt aaatcgcata
      181 ctattcctga cccggtacgg aagttcaggc cactgagttg tgttcctttt ttaaatatta
      241 cccaaaattc atccagaccc aaaattcgcc gggaatttct aagtattctc cgattctgcc
      301 cgaaaacatc gatatttaat tcagatttaa tatgtgaaaa taccgcatat gcgttattgc
      361 gcggccggaa cgatcagtcc acttttgttg tatcttatac accacagctt acccagatca
      421 gtcatatgaa agtaccagtt ggcatagtgg atgacaagtc gcagaaccta cacctgttcg
      481 ctgaacggct aaactattcg gacgacatcg ccattgtgct gacggccggc agcggcgacg
      541 attttctcct ccagatgaag aaactacccc cctccgccca gcccgttgtt atcctgatgg
      601 atatcggcat gccgggtatg gatggcatcg aaacggtacg ggttgccaaa ttactctatc
      661 cgcatatcaa ttttctcatg ctgacggtct tcgacgatga cgataagctc ttcgaggcga
      721 tcaaggcggg cgcggacggc tatttgctca aaaacgacaa gctggctgcc atcatcgact
      781 gcatcgtcca gctctcggag acgggaagcg caccgatgag tccccgcatc gcccggaaaa
      841 cgctcgacct gctgatgaag gcttcgctgc cggagatgga aaacgaaaca caggaaaaaa
      901 aggattatgc cttgtccgat cgggaaacgg aaatactcaa gctgacggtc gatggttacg
      961 cctacaaagc catcgcggaa aggctattcc tcagcagcca taccgtccgg aaacatatcg
     1021 ccaacatcta tatcaaactg cacgtaagtt cgaaggccca ggccatcaaa atagcgacaa
     1081 agaacaagct gatatgattg cattcggcca ctcgggtact gccacaccag cacggcgcgc
     1141 gatggcacca gcctatttgg aaacggtgac cctggtcccc ttcccaggtt cggtttcgag
     1201 cgagagcgta gccccggact ccgccgcccg atgcgtcata ttctccagtc catagtgtcc
     1261 gtaatactgt tgctgccgga caaagccttg tccattgtct tcgatcgtga agcagaagtc
     1321 cacgtcccgt ccggaccgga tactcagaca gatcctttcg gcctgcgaat gccggacaga
     1381 attggcaatc gcctcctgac agatccggaa gacattcagc gcctcgatcg gcgaaaaact
     1441 gtactttttt tgaatatcct cggtgataat ggtctccatt tgcggctgta gcgaagtttg
     1501 cgattgcagg tataatttga gtttgtcggc aaactcatcc agcaggataa attccttttt
     1561 cattgcccag atcgtctccc ggagatcgac caccaggctc ctcgccgtat cattgatgtc
     1621 tgctaattta tccttctctt cttccttgtt gaacgcatgc ggtgtttcca gcagccagtc
     1681 gatggtattg ctgatatggc tcaactgtgt cccgatatta tcgtgcagtt cccgggagat
     1741 ccgctcgcgt tcttttacca cctgctgatc gacttcgagc tttcgcagtt tccagaggta
     1801 tcgctggtgg aggatgaagt agctgatccc gaaaataagg agcaccgtgg caaaggcgga
     1861 gagaagaacg aaccaccagg tcttccacca gggcgggctg atgatgatcc tgatccgttt
     1921 gtggagtgat cgggtcgaat agaggaccgg gctgcaattg atatccagcc aatactttcc
     1981 gggtggaagg atgtagcgga tccctgtcgg ctgcgtagtc gtttgccacg accggtcaaa
     2041 cccctccaac cggtatttat aaagatagtc gctgggattg agcaatccga tagcggccag
     2101 atcgaactga agatgatcct ggttataagg aagccggatc gtatccccct gccaggcatt
     2161 gccaaaagaa gtgtaggccg agtcgttgac ggcgaaccgc acgacagcta ttttcgacga
     2221 atcccgcggt agggaaagct cggatgggta aaaagccgtg atgccattga tgccgccgaa
     2281 gaaaagcctt ccttccggcg acctcgcaca ggatagggtg ttgaattcat tttcctgcag
     2341 ccccagctcc cgcggatagt ttttgatggt gccctctgga aaaatagccg aaagcccgaa
     2401 attggtgctg gcgaagaaac tgttccggct gctatttgcc ggaagcaggg aatagatgat
     2461 gtcattcaac agccctgcct gccgggagag atggcgaacg agccttcccg acctgtcgta
     2521 aataaagatc ccgctctcgg ttcctaccca gatccggtgc atgccatcct cgacgatggt
     2581 gcgggtatag
//
LOCUS       DATGFG010000201         6993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_540056, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000201 DATGFG010000000
VERSION     DATGFG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540056"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VKQ52_03685"
     CDS             <1..1177
                     /locus_tag="VKQ52_03685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ86313.1"
                     /translation="SVFPAYPAGIASLFINQDIGYYGERRLPQKMKIWVAGCGTNQAL
                     FTALKFPDAEVLGTDLSTASLGTCRKNADQIGVTNLRLEERSLNEVQYVEEFDYIIST
                     GVVHHNASPGETLSRISRALKKDGTLELMVYNYYHRLITTACQKAIRCFYDASASYDM
                     KREITILKRIMADFPYDNLLGGYLTSHRAHPEAAIADSFLQPVEYSYTIESLDEMASS
                     SNLEYLNYCINQFDVDGNHLSWNMNFRDPELRALYFSLPDVKRWQISNVLMFNDSPML
                     WFYFQRKDSPYERKVEQDICNAFMESSFSAKPFQLGRYILGEDDRYVLSNNRANATTL
                     MPDDPVVRSVVQTANPQLKMKDVLQRLKIKPDFHTINDIRLKTTTIAYPFLQAVKGW"
     gene            1205..3457
                     /locus_tag="VKQ52_03690"
     CDS             1205..3457
                     /locus_tag="VKQ52_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HLZ86314.1"
                     /translation="MKEYSGLEIAIIGMAGKFPGAADVHAFWDNLANGVESISFFTDE
                     ELAKEGVDRSLIDNPRYVKANGLLENKDCFDSSFFNYRPDEARLMEPQMRIFHECVWA
                     ALEDAGYNPDDEKNKVGLFAGSTTNINWEVYAHLMNRKGYVDNFSVSLLANPRFIPTR
                     ISYNLNLKGPSVYLDTACSTSLVAVHQATKSLLLGECNIAVAGGVHITGRSRTGYMYE
                     EGMIHSRDGHCRTFDNTASGTVSSEGAGVVVLKTLKNALKDGDHIWAIVKGSGVNNDG
                     SDKVGYTAPSIHGQLEAILMAQKWAKTVPESISYIEAHGTATKLGDPVEVEALAHVFG
                     NSTEKYCALGSVKSNIGHLDAAAGVAGLIKTVLALKHRQIPPSLHFQTPNQQINFAGT
                     PFYVNTTLKEWQNDQYPLRAGVSSFGIGGTNAHIILEEAPQLPDAAESRPYQLLLFSA
                     KTPAALERMTEKFRQHLETDKSGAFADIAYTLQTGRKHFPYRKYIVCSSPTEAIQGLA
                     MGKSVTNVEPVADKLSYRKVFMFPGQGSQYVNMCYDLYASEPAFKEEADRCFAIIKQL
                     SGKDLLPVAFNEGGVDAPAIDNTEHTQPLLFVMEYALARSLIKWDIVPDMMIGHSIGE
                     YVAACLSGVFSLEDALWLVIRRGELVQKAPVGAMLSISVSPDALRPLLARYGDVSLAA
                     VNSSSLCVVSGNKEAIQQFRAAVDQEGYENRLLRTSHAFHSCMMDGILDQFRDVVNKI
                     PMHPQQIPFI"
     gene            3668..>6993
                     /locus_tag="VKQ52_03695"
     CDS             3668..>6993
                     /locus_tag="VKQ52_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ86315.1"
                     /translation="MVRSAGDNVNDLHYLMSGVGDLWLHGTAPDWNALYQMGTRRKIS
                     LPAYPFERTRYPVYVDAFRMLTEIGGEDHLQKLPDVSDWLYVPSWKLIPPIVNTVSSR
                     HEGYTLLFADDPGIIRAIGSTREERGQKWIYVTAGDSYCKKADDAYELDPGVKDHFTN
                     MVADLAAHGRMPDRLVHTGDIFSLVETVQAVQQHGGMAGMRVIVLTNELHPILDNGHI
                     AGPGSMTPALLNVLSQEFQNVRTTHIDISLSESGNDAFNTGLCKEIEMAETGAVISLR
                     HGRRWQQVFHRAQPAGTPTAIFREGGVYLITGGLGSVGFSIATNLVVKYHARVVLLGR
                     TTLPPAEKWVSGIQDPGTPEPVRKRIEMLQQLKAAGGEVSYLPCNVGNVAALAAAVRQ
                     CEDTFGKLNGIVHAAGIVEGSSIAPVTELTRERFDAQFEPKIKGLQALKEVAGYKELD
                     FCLIVSSLSAVLGGIGFGAYAPANAFMDHYIRAHKAQGALNNWISVNFDGIDPGKSNT
                     GSINGEELIAVLHRVLAMKEHPGIIVSTSDVYKRIDKWITRKDNAKEDRFPGDELPVD
                     NELPAAFTDAGDGLSDGSGPVIKTMLVLWQNFFGRPDISTGEDFFEAGGDSLKALTMI
                     GRIHKTFNIEIPIKEFFNHSSVAGLARYITRIKAGIANQKGYADYRAIPKAPIQDRYP
                     LSSAQKRLYFLYEFDRASLAYNLPQVIHIKGAINIEIFNHIINVIVSRHEILRTYVDI
                     ADGVPVQRIAGQLTIATEYFCVSAQEAPAIIQEFIRPFDLNKGPLIRTGLIEIAGNEY
                     VFMIDMHHFISDGVSLGIFLKEFMTLYKGNEPGEVPLQYRDYAVWQTSEIYQQAVARE
                     RNWWLQEFSEEIVMLDLPLDHERPMVRNYEGATVHFLLTDAELGSLRRIADAEGVTLF
                     MVILSVYSILLGKLAGQDDVVIGTPTSGRNHADLEQVMGMLVNTVAIRTHPNGALRYR
                     DFLAQLKSKTLTVFDQQAYPYELLIEEVNVARDTSRNPLFDVWFVYQNWEGVRFELPG
                     LDITPRQSGPAISQFDLSLTAGELNGNMLLNFEYSTELFKQQTIERFIAYFRTIIGAI
                     GSDTDRTLGSIALVKGAEQQL"
BASE COUNT         1795 a         1607 c         1907 g         1684 t
ORIGIN      
        1 ctccgttttt cccgcttatc ccgccgggat agccagtctg ttcatcaacc aggacatcgg
       61 ttattacggg gaacgccgtc tgcctcaaaa aatgaaaata tgggtagcag gctgcgggac
      121 aaatcaggcc cttttcaccg cacttaaatt tccggatgcg gaagtgctgg ggaccgacct
      181 gtctacagcc tcccttggaa cctgtcgaaa aaatgccgat cagatcggag taaccaatct
      241 gcgattggaa gaaagaagcc tcaatgaggt gcaatatgtg gaagaattcg actatatcat
      301 cagcacgggc gtagtgcatc ataacgcatc cccgggagaa accctgagca ggatatcccg
      361 ggcgttaaaa aaggacggca ccctcgagct gatggtctat aactactatc accggttgat
      421 aacaactgcc tgtcaaaagg ccatccgctg tttttatgat gcgtcggcgt cctatgatat
      481 gaaacgggag ataaccattc tcaagcggat catggccgat tttccttatg acaacctatt
      541 gggcggctat cttacgagcc atcgggccca tccggaggcc gcaattgcgg acagcttttt
      601 gcagccggtg gagtatagct atacaataga gtctttggac gaaatggcct cctccagcaa
      661 tcttgaatac ctgaattatt gcattaacca attcgatgtt gacggcaatc atttgagctg
      721 gaatatgaat ttccgtgatc ccgaattgcg cgccctctat ttttccctgc ccgatgtaaa
      781 acgctggcag atatccaatg tgctgatgtt caacgattct cccatgctgt ggttctattt
      841 ccagcgaaag gatagtccct atgaaagaaa ggtggaacag gatatctgca atgctttcat
      901 ggaaagcagt tttagtgcaa aaccttttca gctggggcga tacatactag gcgaagacga
      961 cagatatgtt ttaagcaata acagggcaaa cgctacgaca ttgatgcccg atgacccggt
     1021 ggtaagatcc gttgtgcaga cggcgaatcc acagctgaaa atgaaggacg tgcttcaacg
     1081 acttaagatc aaacccgact ttcatacgat caacgatatc cggctgaaaa cgaccaccat
     1141 cgcctatcct ttcctccagg ctgttaaagg ttggtgatag atactactgt ttaaattgac
     1201 tatcatgaag gagtattccg gattagaaat agccattata ggtatggcag gcaagtttcc
     1261 gggggctgcc gatgtgcacg ctttctggga taacctcgcc aacggggtcg aatcgatcag
     1321 tttttttacc gatgaagaat tggcaaagga gggagtggat agaagtctta tcgataaccc
     1381 ccggtatgta aaggcaaatg ggctattgga aaataaggac tgtttcgatt cttccttttt
     1441 caactaccgg ccggatgaag ccaggctgat ggagccgcag atgcgcatct ttcatgaatg
     1501 tgtatgggcg gccctggaag acgccggtta taacccggat gatgaaaaaa ataaggtcgg
     1561 gctgttcgca ggatccacca ccaatatcaa ttgggaagta tatgcccatc tgatgaacag
     1621 gaaagggtat gtggacaatt tttctgtttc attattggcc aacccccggt ttattccaac
     1681 aagaatatcg tacaacctta atctaaaggg gccttccgta tacctggata ctgcctgttc
     1741 cacctccctg gtagctgttc accaggccac taagagctta ttgttgggtg agtgtaatat
     1801 agctgttgcc gggggtgtgc acattaccgg gcgatcgaga acgggatata tgtatgaaga
     1861 aggcatgatc cattccaggg atggccactg ccgcaccttc gacaataccg ccagcggaac
     1921 cgtcagcagt gagggggcgg gggtggtagt cttaaaaaca ctgaaaaacg cactaaaaga
     1981 cggggatcat atctgggcta ttgtcaaggg aagtggcgtc aataatgacg gcagcgacaa
     2041 agtgggctat acagccccct ccattcatgg ccagctggag gctatcctga tggcgcaaaa
     2101 gtgggcgaaa acggtaccgg aaagtatcag ttatatcgag gcgcatggaa cggcgacaaa
     2161 gcttggcgac ccggtagaag ttgaagccct tgcgcatgta tttgggaata gtaccgaaaa
     2221 atattgcgcg ctcggctccg taaaatcaaa tatcggtcac ctggacgccg ccgcgggtgt
     2281 agcggggctt attaaaaccg tgttggcatt aaagcaccgg caaatccctc ccagtttaca
     2341 tttccagacg cccaaccagc agatcaattt tgccgggacc cctttttatg taaatactac
     2401 tttaaaggaa tggcaaaatg atcaatatcc attgcgggcg ggcgtaagtt cgtttggtat
     2461 cggcggaaca aatgcgcata ttatactgga agaagccccg caattgcctg acgccgcgga
     2521 aagccggcct taccagctgc tcctgttctc cgcaaagaca ccggcggcgc tggaaagaat
     2581 gacagaaaaa ttccgtcagc accttgagac ggataagagc ggcgcctttg ccgatatcgc
     2641 ctacacgctt caaacggggc gcaaacattt cccttacagg aaatatattg tgtgcagcag
     2701 ccccaccgaa gccattcagg ggcttgccat gggcaaatca gtcacaaatg tggaaccggt
     2761 tgccgataag ctgtcgtacc gaaaggtatt tatgttcccg ggccagggtt cgcagtatgt
     2821 aaacatgtgt tatgacctgt atgcgagtga gcctgctttt aaggaagaag cggacaggtg
     2881 ttttgccatc attaagcagc tatcgggtaa agacctgttg ccggtggcct tcaatgaagg
     2941 gggcgtggat gctcctgcga tagataacac ggagcatacc caacctttgt tgtttgtaat
     3001 ggaatatgca ttggcccgat cattgataaa atgggatatc gttcccgata tgatgatcgg
     3061 tcacagcatt ggggaatatg tagccgcctg tcttagcggg gtattttccc ttgaagacgc
     3121 cttatggctg gtgatcaggc ggggagagtt ggtgcagaag gccccggttg gggctatgct
     3181 gagtatttcc gtttcacccg atgccctgcg cccgttgctt gcccggtacg gggatgtatc
     3241 cctggcggct gtcaacagct catcgttatg tgtggtatcg ggtaacaagg aggcgataca
     3301 acagtttaga gcggcggtgg accaggaggg gtatgaaaac aggttactcc gcacttcgca
     3361 tgcctttcac tcctgcatga tggatggtat ccttgatcaa ttcagggatg tcgttaacaa
     3421 aataccgatg cacccgcaac agataccctt tatttagaac ctaagcggca agcgggcgac
     3481 cgatgcggaa atatcccgtc cgggctattg ggttgaccac ctgaggaaca ccgttcaatt
     3541 ctccgccggt atacagacct tactggaaaa tgaccgggtg ctgtttattg aagtcggtcc
     3601 gggcagacag ttgtgcacta tggtccggtc gaatcaaata cgcaaaaaga cacatacctg
     3661 ttgtcaattg gttcgaagtg cgggagacaa tgtcaatgac cttcattacc tgatgtcggg
     3721 ggtcggggat ctttggctgc atggaacggc gcctgactgg aatgcactgt atcaaatggg
     3781 aacccggaga aaaatatccc ttcctgctta tcccttcgaa aggaccaggt atcctgttta
     3841 tgtggatgcc ttcaggatgt taaccgagat aggaggggag gatcatttgc agaagttacc
     3901 ggatgtgtca gattggttgt atgtgccgtc ctggaagctt atcccaccta ttgtcaatac
     3961 cgtttccagc cggcatgaag gctatacgtt gctcttcgcc gatgatcccg gaataatccg
     4021 ggcgatcggt tcaacaaggg aagagcgagg acagaaatgg atttacgtaa cggccggtga
     4081 ttcctattgt aaaaaagcgg acgatgcata tgaattggat ccgggcgtaa aagatcattt
     4141 taccaatatg gtcgccgacc tggcggcaca tggccggatg cctgaccgcc ttgttcatac
     4201 aggggacata tttagccttg ttgaaacggt gcaggcagtg caacagcacg gaggtatggc
     4261 cggtatgcgg gtgatcgtgc ttaccaacga attacaccca attctcgaca acggtcatat
     4321 tgccggcccg gggtcaatga cgcctgccct gttaaatgta ttgtcgcagg aatttcaaaa
     4381 tgttcgtaca acccatatag atatcagctt gtccgaatcg ggaaatgacg ctttcaacac
     4441 gggattgtgt aaagagatag agatggccga aacgggagcg gtcatctctc ttcgtcatgg
     4501 aaggcggtgg cagcaggtgt ttcaccgggc tcagcccgca ggcacgccca cagctatttt
     4561 ccgcgagggt ggggtgtacc tgatcaccgg tggcctggga agcgttggat tcagcatagc
     4621 aacgaacctg gttgtaaaat accatgcccg ggtggtactg cttggccgaa cgacgctgcc
     4681 tccggctgaa aaatgggtga gcggtattca ggaccctggt actccggagc cggtacggaa
     4741 aaggatagaa atgctgcagc agctgaaagc cgccggtggc gaagtatcgt acctgccgtg
     4801 taatgtgggg aatgtggcgg cgctggctgc cgcggtgcgg caatgtgaag atacattcgg
     4861 gaaattaaat ggcattgtac atgccgccgg tattgttgaa gggtcgtcta ttgcgccggt
     4921 gacggagctt accagggaaa ggttcgatgc acaattcgag cccaagataa aagggttgca
     4981 ggcgttgaaa gaagtggcag gctataaaga acttgacttt tgcctgatcg tatcttcgct
     5041 ctccgccgta ttgggtggaa taggttttgg agcgtatgcc cctgccaatg cctttatgga
     5101 tcattatatc cgggcacata aggcgcaggg cgcattaaac aactggatca gtgtgaattt
     5161 tgatggcatc gatcccggga aaagcaatac cggcagcatc aacggcgagg aattgatagc
     5221 ggtgcttcac cgggtactgg ctatgaagga gcacccggga attattgtat ccacctccga
     5281 tgtttataaa cgtatcgaca agtggataac caggaaggac aacgcgaaag aagaccgttt
     5341 tccgggcgac gaacttcctg ttgacaacga acttcctgca gcctttacgg atgcgggcga
     5401 tggcctttcg gatggttccg gtccggtgat aaagacaatg ctggttttat ggcagaattt
     5461 ctttggcaga ccggacatca gcaccgggga agacttcttt gaagcaggcg gtgattccct
     5521 gaaggcattg acgatgatcg gtcgcattca taagactttc aatatcgaga tccccatcaa
     5581 ggagtttttt aaccattcat ctgttgccgg tctggcccga tatataaccc ggataaaggc
     5641 cgggatagcc aatcaaaaag ggtatgccga ttaccgggct attcccaaag caccgatcca
     5701 ggacaggtat cccctgagtt cggcgcaaaa gcggttgtat ttcctatacg aattcgatcg
     5761 ggcttcgttg gcttacaacc tgccccaggt gatccatatt aaaggggcga taaacataga
     5821 aatattcaac catataatca atgtcatcgt ttcccgccat gaaatactaa gaacttatgt
     5881 agatatagca gatggagttc ctgttcagcg gatagcaggg cagctaacta tcgctacgga
     5941 atatttttgt gtgtctgcgc aggaggcgcc ggctattata caggaattta tcaggccgtt
     6001 tgatctgaac aaaggaccgt tgatcaggac cggattgata gagattgccg gaaatgagta
     6061 tgtgttcatg atcgatatgc atcattttat ctcggacggg gtttcactcg gcatcttttt
     6121 aaaggagttt atgacccttt ataaaggaaa tgagccgggg gaagtgccat tgcaataccg
     6181 ggactatgcc gtgtggcaaa ccagcgagat ctatcagcag gctgttgccc gcgaaaggaa
     6241 ctggtggctg caggaattca gtgaagagat agtcatgctg gatcttccgc tcgatcacga
     6301 acggccgatg gtacgaaact atgaaggagc gacggtccat tttttactaa ccgatgcgga
     6361 attgggaagc ctgagaagaa tagccgatgc cgaaggggtt acgctcttta tggtgatatt
     6421 gtcggtttac agcattttac tggggaaact ggcagggcag gacgatgtgg tgatcgggac
     6481 cccgacctcg ggacgcaatc atgccgacct ggagcaggtg atgggtatgc ttgtgaatac
     6541 ggtggccatc cgtacccatc caaacggcgc gttgcgttac cgtgattttt tggcgcagct
     6601 gaaatcaaag acacttaccg tcttcgacca acaggcgtat ccatatgaat tattgataga
     6661 agaggtgaac gtggcgcgtg atacgagccg gaatccctta tttgacgtgt ggtttgtata
     6721 ccagaactgg gaaggtgtac gatttgagct acccgggctg gatatcactc ctcgtcaatc
     6781 gggtccggct atctcgcagt tcgatctgtc cctgaccgcc ggcgagctga atgggaatat
     6841 gctgctgaac ttcgaatact ctacggaact tttcaagcag cagacgatcg agcgatttat
     6901 cgcttatttc cgcactatta tcggcgccat cggcagcgat acggatcgta cgcttggttc
     6961 tattgcgctg gtgaaggggg cggaacagca act
//
LOCUS       DATGFG010000202         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1565980, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000202 DATGFG010000000
VERSION     DATGFG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565980"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /gene="deoC"
                     /locus_tag="VKQ52_03700"
     CDS             complement(<1..426)
                     /gene="deoC"
                     /locus_tag="VKQ52_03700"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HLZ86316.1"
                     /translation="MSRLARYIDHTILKPTTSHIEISKICGEAVEYGFAAVCVPPPMV
                     SFALQELGGRGGVKVATVIGFPFGYSVAAAKLAETERALADGADELDVVINLIALKAG
                     DWALLEAEMLTLTKRIHAAGKIVKVIIESGVLTDEEIIRC"
     gene            534..>3276
                     /locus_tag="VKQ52_03705"
     CDS             534..>3276
                     /locus_tag="VKQ52_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ86317.1"
                     /translation="MRNSRHRPRGRILVLSATLLSLTLFSSAQTTFPVNGVADAREHC
                     YAFTHATLVKDGATTVNDATLIIRDGLIVSAGAGAAIPKDAVVIDCSGKYIYPSFIDL
                     YTDYGIPVPEGVRTRGPLSPADFFRQPAQIESATKGAYNWNQAVHPETDASRLFNVDD
                     AKAKPMRDIGFGTVLSHVKDGIARGTGVVVTLANDNENLVIVKQKASAHYSLNKGTST
                     QSYPSSLMGSIALLRQTYIDAAWYKSQPYHEGTNLSLQAWNDEQTLPQIFDGSDKWGD
                     LHGDRIGDEFGVQYIIKAGENEYQRIKEIAATKASFIVPLDYPQAMDVEDADNARFVS
                     LADMKHWELAPTNPGAFEKAGIPFCLTTADLRDTKTFFANLRKAFDFGLTETKALEAL
                     TKTPATLIGIYDKVGSLDAGKLANFLITTGPVFNEKTNIVENWVQGKKYDVKEDAWKD
                     VKGQYNLVITSTTGTSSTLVLDVKSSTAASVIGKDTLTGKFSYDGKLVSLSFGGPAEA
                     AGRGGRGGAPGASPAGGNPPAAGEAPAGGRRSGNGGRPSAATRLSGVVNGDTWNGNGT
                     DSTGNLVIWTATFAKATPAVADSTPRRRPNRVGRVLYPFDGYGWDSLPTQHDLLIKNA
                     TVWTNENEGRLANTDVLVKAGKIAKIGKGLDAGAAQVIDGTGKWLAPGIIDEHSHIAA
                     FSINEGAQSVTSEVRIADNLNPEDISIYRQLAGGVTSSHILHGSANTIGGQTQLIKLR
                     WGMDDEGLKFRNWDPFIKFALGENVKRTTSQNNNRFPDTRMGVEEVLTDAFTRARDYQ
                     RLRKTAPNVTRRDLELDALVEILEHKRFITCHSYVQSEITALLRVADKFGFKVNTFTH
                     ILEGYKVADKMKEQGCSASTFSDWWAYKMEVQDAIAYNATIMNKVGLNVCINSDDAEQ
                     "
BASE COUNT          787 a         1088 c          806 g          595 t
ORIGIN      
        1 acaccggatg atctcctcat ccgtcaacac cccgctttcg atgatgacct ttacgatctt
       61 cccggcggca tgtatccgct tcgtcagcgt cagcatctcc gcttccagca aggcccagtc
      121 ccctgccttc agggcgatca ggttgatcac cacgtccagc tcatcggccc catcggcaag
      181 cgcccgctcc gtttccgcca gcttcgccgc cgcaacggaa tacccaaaag gaaacccgat
      241 caccgtcgcc accttcacgc ctccacgccc gcccagctcc tgcagggcaa acgaaaccat
      301 cggcggcgga acacagaccg ccgcaaaccc atactctacc gcctctccac atatcttact
      361 tatctctata tgagaggttg tgggtttcaa aatggtatgg tcaatatagc gtgcgagcct
      421 actcaccgac tttttgctcc tgttttaccg ttgataaaat attcaaaccc taaaattact
      481 ccatttacct aaattcggga ccatttttca aactgactaa aaactaacaa aacatgagaa
      541 attcccgaca tcgtccgagg ggaaggattc ttgtcctctc tgctacactt ctctctctta
      601 cgcttttttc atccgcgcag accactttcc ctgtcaacgg tgtcgccgat gcccgggaac
      661 actgttatgc ctttacgcat gccaccctgg taaaggatgg cgccaccacg gtcaacgacg
      721 cgaccctgat catccgcgac ggcctgatcg tcagcgcagg tgcgggcgcc gccattccta
      781 aggacgctgt cgtcatcgac tgcagcggga aatatatcta cccctccttt atcgatcttt
      841 ataccgacta tggcatcccg gtccccgaag gcgtccgcac ccgtggtccc ctgagtccgg
      901 ccgacttctt ccggcagcct gcccagatag aatccgctac aaagggcgct tataactgga
      961 accaggccgt ccaccccgaa acggacgcct cccgcctctt caatgtcgac gacgcaaagg
     1021 caaagcccat gcgcgacatc ggctttggaa ccgtcctctc gcatgtgaag gatggtatcg
     1081 cccgcggaac gggcgtggtc gtcaccctgg ccaacgataa cgagaacctt gtcatcgtca
     1141 aacaaaaagc ctccgctcat tattcgctga acaaggggac ttccacccag agctacccct
     1201 catcgctgat gggttccatc gccctgctgc gccagaccta tatcgatgcc gcctggtaca
     1261 agagccagcc ctaccacgaa ggtacgaacc tcagcctcca ggcctggaac gacgaacaga
     1321 ccctgcccca gatcttcgat ggcagcgaca aatggggcga cctccacggc gatcgcatcg
     1381 gtgacgaatt cggcgtacag tatattataa aggccggaga gaacgaatac cagcggataa
     1441 aggagatcgc cgcgacaaag gcctccttta tcgttcccct cgactacccc caggccatgg
     1501 acgtcgaaga tgcggataac gcccgcttcg tctccctcgc tgatatgaaa cattgggaac
     1561 tcgcacccac caaccccggc gcattcgaga aggcaggcat tccattctgt ctcacaacgg
     1621 ccgatctccg ggatacaaaa acgtttttcg ccaacctccg caaggccttc gacttcggtc
     1681 tcaccgaaac aaaggccctc gaagcgctga caaagacccc ggccaccctc atcggcatct
     1741 atgacaaggt cggctccctc gacgccggca aactcgcaaa cttcctgatc accaccggac
     1801 cggtcttcaa tgaaaagacc aatatcgtgg agaactgggt ccagggaaag aaatatgatg
     1861 taaaagaaga tgcctggaaa gacgtaaaag ggcaatacaa cctggtgatc acctccacta
     1921 ccggtacttc cagcacgctc gtcctcgatg taaaatcttc gacggcggcc agcgtcatcg
     1981 gcaaggatac ccttacggga aaattcagct atgacggcaa actggtctcc ctcagctttg
     2041 gcggccccgc cgaagcagcg ggccgtggtg gtcgtggcgg cgcacccggc gccagtcccg
     2101 ccggcggcaa ccctcccgct gccggtgaag cacccgcggg cggaagaaga tccggcaatg
     2161 gcggacgccc ctcagccgca acccggctca gcggcgtcgt caacggcgac acctggaacg
     2221 gcaacggcac cgactccacc ggaaacctcg ttatctggac ggcgactttt gcaaaagcga
     2281 ccccggcggt ggccgacagc acgccccgca ggcgccccaa ccgcgtcgga agagtgctct
     2341 accccttcga cggctatggc tgggatagcc tcccgaccca gcatgacctg ctcatcaaaa
     2401 acgcaaccgt ctggacaaac gaaaatgaag gccggctggc caacaccgat gtactcgtaa
     2461 aggcggggaa gatcgcaaag atcggcaaag gactcgatgc gggcgccgcc caggtcatcg
     2521 acggcaccgg taaatggctc gcccccggga tcatcgacga gcactcccat atcgccgcct
     2581 tctccatcaa cgaaggcgca cagtccgtca cctccgaagt gcgcatcgcc gacaacctca
     2641 accctgaaga catcagcatc taccggcaac tggccggggg tgtaaccagc agccatatcc
     2701 tccacggctc agccaacacc atcggcggac agacccagct catcaagctg cgctggggga
     2761 tggatgacga agggctgaaa ttcaggaact gggacccctt catcaaattc gccctcgggg
     2821 agaatgtaaa acgcaccacc tcccagaata acaaccgctt ccccgacacc cggatgggag
     2881 tggaagaggt gctgacggac gcctttaccc gcgcccgcga ttaccagcgg ctgcgcaaga
     2941 cggcgccaaa cgtaacccgc cgggatctgg aactcgatgc ccttgtcgag atcctcgaac
     3001 acaaacgctt tatcacctgt cactcctatg tccagagcga gatcaccgcg ctcctgcggg
     3061 tggcggataa attcgggttc aaggtcaata ccttcaccca tatcctcgaa ggctataagg
     3121 tcgccgataa gatgaaggaa cagggttgca gcgcctccac cttctccgac tggtgggcgt
     3181 acaaaatgga agtacaggac gccatcgcct ataatgccac gatcatgaac aaagtaggcc
     3241 tcaacgtctg tatcaactcc gacgatgccg aacagg
//
LOCUS       DATGFG010000203         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1565946, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000203 DATGFG010000000
VERSION     DATGFG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565946"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2765)
                     /locus_tag="VKQ52_03710"
     CDS             complement(<1..2765)
                     /locus_tag="VKQ52_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86318.1"
                     /translation="MLLSRLPLLLGALLLSTGMYAQVFGGNPPSLKWRQLNTDTARVI
                     FPIGLDSQAQAVAAIVHRLARTTRPTIGPRQHKIDIVLQNQTTIANGYVSLAPFRSEF
                     QLTPLQNSFDLGSLPWPKMLAIHEYRHVQQYNNFRVGLSGAFYYLFGEGGQAFANSLA
                     VPNWFWEGDAVYQETLVSDQGRGRLPLFLDSYRSLWTAHRRYSWMKLRNGSLRDYVPD
                     HYPLGYMLIAYGRERYGDDFWRQVGHDAAAFHGLFYPLQGAVRRYAGIPYRQFRSDAL
                     DFFRRQAISDTVSEAEQRAARAHHFIANQEFPQFIGRDSLLYMRSTYKQIPAFVLRNT
                     ANGTDAVIRTRAISLDNYFSFAGRHVVYAAYETDPRWGWRDYSVIRLLDPWTGEDKRI
                     TSRSRYFAPDISANGRQIVAVQEAADGSCELHLLDTTGNLLSRLPNPDQLFYTYPKFF
                     GATAIVTAVRNRKGEMSLALVNTTGGGVRYLLPFSFQTIGFPSVSGDTVWFNASREGR
                     DRVYALVGDRIFRVWLPNGAAVTGQYQFHSGADGRFAWNTFTAGGFLMDASGRPGLSP
                     VDSRDWVRELPVQHIDSLEKGPSRLLDRITPGNYPATAYPLASHLFNFHSWRPYINDP
                     DYQLSLVSNNILNTFETQLYGDYNRNEQYKQVGVTATYGGLYPFLDAGWDYTIDRNAY
                     YRGQKVFWNESETRAGFSIPLNWASHLNYTSLQFGSGIVYNQRYYSGTYKDSFNNRGF
                     AYVDPYISFVHQSQQAQQQIAPRLAQVLTLSYSSAVTAFTAHQFLASGFLYLPGLAYT
                     HSLQLAAAFQQRDTLGNARFSNSFPFSRGYTAENFYRMWRVSGNYQFPLLYPDWGFGN
                     IVYFLRVRTNLYYDFTRAMDFDNLGHTYNWNFRSFGSEVYFDTKWWNQLALSFGIRYS
                     RLLDPDF"
     gene            2827..>3189
                     /locus_tag="VKQ52_03715"
     CDS             2827..>3189
                     /locus_tag="VKQ52_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86319.1"
                     /translation="MKINFRRLVLSGNYDAVPAAIAGFLIIQALCAYGGIGVSPDSVV
                     YISTAQNIHDHGAINDFSNMPVMDFPAFYPIFLSGLIFLTGSKVVSLGPALDGLLFAT
                     LIGLCGWIMNRFTRVTRTY"
BASE COUNT          662 a          855 c         1037 g          635 t
ORIGIN      
        1 tcgaagtcgg ggtcgagcag ccggctgtag cggataccga agctcaatgc caattggttc
       61 caccatttcg tatcgaaata gacctcggag ccgaaggagc ggaaattcca gttataggta
      121 tggccgaggt tatcgaaatc catggctcgg gtaaaatcat agtacaggtt ggtccggacg
      181 cggaggaaat acacgatatt cccgaaaccc cagtcgggat agagcagcgg gaactggtag
      241 ttacccgaaa cccgccacat ccggtaaaaa ttttcggctg tatatccccg ggaaaagggg
      301 aagctgttgg agaaccgcgc gttccccagc gtatcccgtt gctgaaaagc agcggcaagc
      361 tgaaggctgt gggtataggc gagcccgggc aggtacagga aaccggaagc caggaactgg
      421 tgggccgtaa aggccgtcac ggcgctgctg tacgagaggg tgagcacctg cgccagccgg
      481 ggcgctatct gttgctgtgc ctgctgggat tgatggacaa agctgatata cggatcgaca
      541 taggcgaatc cgcggttgtt gaatgaatcc ttgtatgttc ccgaatagta ccgttgattg
      601 tagacgatac ccgacccgaa ctgcaggctg gtatagttca aatggctggc ccagttgagc
      661 gggatgctga acccggcccg ggtctcgctc tcgttccaga acaccttctg gccgcgataa
      721 taagcattgc gatcgatcgt atagtcccat cccgcatcga ggaaggggta gagtccccca
      781 taggtggccg tcacgcccac ctgcttatac tgttcgttgc ggttgtaatc cccatacaac
      841 tgggtttcga aggtgttcag gatattgttg ctcacgaggg atagctggta atcggggtcg
      901 ttgatatagg ggcgccagct gtgaaaattg aacaggtggg aggcgagcgg gtaggccgtc
      961 gcgggatagt tgcccggtgt gattcggtcc agcagccggg agggcccttt ttcgagggaa
     1021 tcgatatgct gtaccggcag ttcccggacc cagtcgcggc tgtcgacggg cgataacccg
     1081 ggccggccgg atgcgtccat caggaagccg cccgccgtga aggtattcca tgcaaagcgg
     1141 ccgtcagcgc cggaatggaa ctggtattgc cccgttacgg cggcgccatt gggcagccat
     1201 acgcggaaga tccgatcgcc caccagggca tagaccctgt ccctgccttc ccgtgaagcg
     1261 ttgaaccaga cggtatcgcc gctgacggag ggaaagccga tggtctgaaa actgaagggc
     1321 agcaggtaac ggacgccgcc gccggttgta ttcaccaggg caagcgacat ttccccttta
     1381 cggttacgca cggccgttac gatcgcggtg gcgccgaaga acttgggata ggtgtagaaa
     1441 agctggtcgg gattggggag ccggctgagc aggttgccgg tggtatccag gagatgcagt
     1501 tcgcaggagc cgtcggcagc ctcctgtacg gcaacgatct gccggccgtt ggcggagata
     1561 tccggtgcaa aataacggct gcggctggtg atccttttgt cttctcccgt ccaggggtcc
     1621 agcaggcgga taacgctgta gtcccgccag ccccagcgcg ggtcggtctc gtaggcggcg
     1681 tagaccacgt gccggccggc aaaggagaaa tagttgtcca gggagatcgc ccgggtccgg
     1741 atgacggcat ccgtgccgtt ggccgtgttc cgcagcacaa aggccggtat ctgcttatag
     1801 gtgctgcgca tatacaggag gctgtcccgg ccgatgaatt gggggaactc ctggttggcg
     1861 ataaaatgat gcgcccgggc agcccgctgt tcggcctcgc tgaccgtgtc cgatatcgcc
     1921 tggcggcgga aaaaatcgag tgcgtcgctg cgaaactgcc ggtaagggat cccggcatag
     1981 cgccggaccg ctccctgcag ggggtagaag agcccgtgaa aggcggcggc atcgtggccc
     2041 acctgccgcc agaaatcgtc gccatagcgt tcacggccat aggcgatgag catatagccc
     2101 agggggtagt gatcggggac atagtcccgc agggagccat tgcgcagttt catccaggaa
     2161 tagcgcctgt gggcggtcca cagggaacga tagctgtcga gaaacagggg aagacggccg
     2221 cggccctggt cgctgacgag ggtctcctgg taaacggcat ccccttccca gaaccagttg
     2281 ggcaccgcca ggctgttggc gaaggcctgg ccgccctcgc caaagagata gtagaaggcg
     2341 ccggaaaggc ccacgcggaa attgttgtat tgctggacgt gccggtattc atggatggcc
     2401 agcatctttg gccagggcag gctaccgaga tcgaagctgt tctgcagagg ggtgagctgg
     2461 aattcgctgc ggaacggcgc caggctgacg tagccgttgg cgatggtggt ctggttctga
     2521 aggacgatat cgatcttgtg ttgccggggc ccgatggtag gccgggtggt ccgggcgagc
     2581 cggtggacga tggcagccac tgcctgcgcc tgcgagtcca ggccgatagg gaaaatgaca
     2641 cgcgcggtat cggtgttgag ctgacgccat ttcagggaag ggggattacc gccaaatacc
     2701 tgggcgtaca tacctgtcga cagcaaaagc gctccgagga gcaggggcag gcgggagaga
     2761 agcatgtaat tggttcactt tttggtttac aggtggctca aattaagtat ttttcccatc
     2821 aaatctatga agatcaattt ccgaagatta gttctatccg gcaactatga tgctgttccg
     2881 gcggccatcg ccggattcct gatcatccag gcgctttgcg cctatggcgg catcggggtc
     2941 tcccccgact cggtcgtcta tatcagcacg gcccagaaca tccacgatca cggcgcgatc
     3001 aacgatttca gcaatatgcc ggtgatggat ttccccgcct tctaccccat cttcctcagc
     3061 gggcttattt tccttaccgg cagcaaggtg gtcagcctcg ggcccgcgct ggacgggttg
     3121 ttgtttgcga ccctgatcgg gctttgcggg tggatcatga accgcttcac ccgggtcacc
     3181 aggacctac
//
LOCUS       DATGFG010000204         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_648769, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000204 DATGFG010000000
VERSION     DATGFG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648769"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VKQ52_03720"
     CDS             <1..295
                     /locus_tag="VKQ52_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86320.1"
                     /translation="HVPLERAIMAIRFNDHMIGTQFHPEADATGMSMYLRREDRKATV
                     IAEHGEEKWKSMIEQLDDPDKIRYTYSHIIPNFLDDALRPARKREAQRLRKTA"
     gene            315..1514
                     /locus_tag="VKQ52_03725"
     CDS             315..1514
                     /locus_tag="VKQ52_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86321.1"
                     /translation="MVPALRKAFNEAFTRQRYEQFLTHLNSRYPGAIEFRIAETPVFI
                     PAAFARLLSEACEHIVDLILEPKFREWTQRAIPRQDYVARENDHPHFLVFDFGICEDG
                     HGGYQPQLIEMQGFASLYGFQVYYPETLRRHFSIPDNYSQFLNGYDKESYLTLLRQTI
                     LGDCPPEEVILLEIKPHEQKTRIDFYCTRDYLGIQPVCLSELIQEGKKLYYMHPVTGK
                     KTLIRRIYNRLIFDELHAAAAKLPPGFVDIRQELDVEWVAHPNWFYRISKFTLPFIRH
                     PFVPPTFFLDELKQPPADLENYVLKPLFSFAGQGVIIDVRPEDLDVPDPANWILQRKV
                     KYAGVIATPDVPAKAEIRMMYLWKDGDPRPTPAINLARLSKGKMIGVNYNKDKEWVGG
                     SVGFFER"
     gene            complement(1515..1922)
                     /locus_tag="VKQ52_03730"
     CDS             complement(1515..1922)
                     /locus_tag="VKQ52_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86322.1"
                     /translation="MEKVLQIIAVFALIMLIIHRFIRGREPAYRGYNYLTRLQPPLIA
                     EVGQRLITAVFGAGTEDVPGQVDAFILDEERQLLFAYSAVGSLTIYRRKEGDGFKEMQ
                     WLAAPLNCTGMALDPQDGKVYLEAGGFLFVYGA"
     gene            1999..>2224
                     /locus_tag="VKQ52_03735"
     CDS             1999..>2224
                     /locus_tag="VKQ52_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ86323.1"
                     /translation="MDLQLTGKTALVLAASKGLGKASALALASEGADVIIGARDRATL
                     ESTAAELRALGKGRVMAIPVDVTNAQQIKEM"
BASE COUNT          534 a          746 c          529 g          415 t
ORIGIN      
        1 ccatgtcccc ctcgaaaggg ccatcatggc catccggttc aatgatcata tgatcggcac
       61 ccagttccac cccgaagcag atgccacggg tatgagcatg tacctccggc gcgaagaccg
      121 caaggccacc gtcatcgccg aacacggcga ggagaaatgg aagagcatga tcgagcagct
      181 ggacgacccc gacaagatcc gctacaccta ctcccatatc atccccaatt tccttgacga
      241 tgccctccgt ccggcaagga agcgcgaagc acagcgtctg aggaaaacag cgtaacttcc
      301 gggaaaagac acccatggta ccagcactgc ggaaagcctt caacgaagcc ttcacccggc
      361 aacgctatga acagttcctg acccatctga acagccgata cccgggcgcc atcgagttcc
      421 gcattgcaga aacccccgtc ttcatacccg ccgcgttcgc ccgcctcctt tcggaagcct
      481 gcgaacatat cgtcgatctc atcctcgaac caaaattcag ggagtggacc caacgcgcca
      541 taccccggca agactatgtt gcccgtgaga acgaccaccc gcacttcctg gtcttcgact
      601 tcggcatctg cgaggatgga catggcggct accagcccca gctgatcgaa atgcagggat
      661 tcgccagcct ctatggcttc caggtctact accccgaaac cctccgccgc catttctcca
      721 tacccgacaa ctatagccag ttcctcaatg gctacgacaa ggaaagctac ctcaccctcc
      781 ttcgccaaac cattcttggc gactgcccgc ccgaagaagt gatcctgctc gagataaaac
      841 cccacgagca aaagacccgg atcgattttt actgcacccg cgattatctc ggcatccaac
      901 ccgtatgcct gtccgaattg atccaggaag ggaaaaaatt gtactatatg cacccggtca
      961 ccggcaaaaa gaccctcatc cgccgcatct acaaccggct catcttcgac gagctgcacg
     1021 ccgccgccgc aaaactgccc ccgggttttg ttgatatccg ccaggaactg gatgtcgaat
     1081 gggtcgccca tcccaactgg ttctatcgca tcagcaagtt caccctgccc ttcatccgtc
     1141 atcccttcgt gccgcctacc ttcttcctcg acgaactgaa gcaaccgccg gccgacctcg
     1201 aaaattatgt cctcaaaccc ctgttctcct ttgccggcca gggagtgatc atcgacgtcc
     1261 ggcccgaaga cctggatgtc ccggatcccg ccaactggat cctgcaacgc aaggtgaaat
     1321 atgccggggt catcgccacc ccggacgtcc cggccaaggc ggagatccgg atgatgtacc
     1381 tgtggaagga cggcgatccc cgccccaccc ccgccatcaa cctggcgcgg ctgagcaagg
     1441 gcaagatgat cggtgtcaac tacaataaag acaaggaatg ggtgggcggc agcgtggggt
     1501 tcttcgaacg ctgatcacgc cccgtaaaca aaaagaaagc cgccggcttc caggtagacc
     1561 ttcccgtcct gtgggtccaa tgccatcccc gtacagttca gcggcgcggc cagccattgc
     1621 atctctttaa aaccatctcc ttccttccgc ctgtaaatgg tcagactccc cacggcactg
     1681 taagcgaaca acaactgtcg ctcctcatcc aggataaagg catccacctg gcccggcacg
     1741 tcctccgttc ccgcaccaaa caccgccgtt atgagccgct gccctacttc ggcgatcaat
     1801 ggcggctgca gccgggtaag ataattgtac ccccggtagg ccggctcgcg gccccggatg
     1861 aaccggtgaa tgatcaacat gatcaaagca aacaccgcga ttatctgtaa taccttttcc
     1921 atcggctcta atgtctgtac acttcgtgta cgaaccggat tttatccggt tcagcaatat
     1981 accccgtatc tttagaatat ggatcttcaa ctcaccggaa aaaccgccct cgtgctcgcc
     2041 gcaagtaaag gattgggaaa agcaagcgcc ctcgcgctcg cgtcggaagg cgcagatgtg
     2101 atcatcgggg cccgcgacag ggctaccctc gaatcaacgg ccgcagaact ccgcgccctc
     2161 gggaagggcc gcgtaatggc gatcccggtc gatgtcacca atgcccaaca aataaaagaa
     2221 atga
//
LOCUS       DATGFG010000205         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_763522, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000205 DATGFG010000000
VERSION     DATGFG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763522"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VKQ52_03740"
     CDS             <1..685
                     /locus_tag="VKQ52_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLZ86324.1"
                     /translation="REKTTDPRPQIDLVKKQLAVEQSQLEQQLREKARTENLVKADAA
                     TQKQLDDINSLIDQLQKQINVTRQQLAVQETNIANQNRSVLSEKAPLEKSAAQFEDQI
                     RKGVIINPQRGTVLARYALQGEMSTVGKALYKIADLDTLNLKAYITGTQLPQVRLGQQ
                     VTVRIDAGSKSYRQYPGAITWISDKSEFTPKTIQTKDERANLVYAIKVRVKNDGFLKI
                     GMYAEVKLQ"
     gene            721..1749
                     /locus_tag="VKQ52_03745"
     CDS             721..1749
                     /locus_tag="VKQ52_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ86325.1"
                     /translation="MKAVTLDHITKTYDKGRVTAVKDISFDVQEGELFGLIGPDGAGK
                     STIFRILTTLLLADKGTATVDGLDAVKDFRQVRGCVGYMPGRFSLYQDLTVAENLNFF
                     ATLFGTSIEKNYALIADIYEQIKPFSTRRAGKLSGGMKQKLALCCALVHKPRVLFLDE
                     PTTGVDAVSRKEFWDMLKRLKSQGITILVSTPYMDEANLCERIALIQEGRILSIDSPG
                     NIVKAYPDKLYAVKADSIPRLLHDARLFPETLSCYAFGEYLHVSFKPGETATTQAPHR
                     MEQYLGEKGHTNIRLEEIAATIEDSFIQLMGTHSEPSDHDNPSDHNNPSNHDRSIDPA
                     NSSNLNNR"
     gene            1751..>2154
                     /locus_tag="VKQ52_03750"
     CDS             1751..>2154
                     /locus_tag="VKQ52_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ86326.1"
                     /translation="MNDKAIICENLRKQFGDFIAVNDISFEVNAGEIYGFLGANGAGK
                     TTAMRMLCGLSYPSGGHATVAGHNVYKEQESIKKKIGYMSQKFSLYENLTIRENIEFY
                     GGIYGLSDKDLKQKGDELINRLGLANEARKLVG"
BASE COUNT          642 a          630 c          509 g          373 t
ORIGIN      
        1 ccgggagaaa acgacagacc cccgcccgca aatcgacctg gtaaaaaaac aactggcagt
       61 ggaacagtca caactggagc agcaattgag agaaaaggcc cggaccgaaa acctggtcaa
      121 ggcagacgca gccacacaaa aacaactgga tgatatcaac tcgctcatcg atcagctgca
      181 aaagcagatc aatgtcaccc gccagcaatt ggccgtacag gaaaccaata tcgccaacca
      241 gaaccggagc gtgctgagtg aaaaagctcc cctggaaaaa tcggcggccc aattcgaaga
      301 tcagatccgc aaaggcgtca tcatcaaccc ccaacgcggc accgtgctgg cccgctatgc
      361 gctgcaaggc gaaatgagca cagtaggcaa ggccctctac aagatcgccg acctcgatac
      421 actcaactta aaagcataca tcaccgggac ccagctgcca caggtccggc tcggacaaca
      481 ggtgacagta aggatcgatg caggcagcaa gtcctacagg caatatcccg gcgccatcac
      541 atggatctcc gacaaatcgg aattcactcc gaagactatc cagaccaagg acgaaagggc
      601 caacctcgta tatgccatca aagtcagggt gaaaaatgac ggcttcctga aaatcggtat
      661 gtatgccgaa gtaaaattgc aataacccca tcccccaccg gaaacaaaac aatttaaagg
      721 atgaaagcag taaccctcga tcacatcacc aaaacctacg acaaaggcag ggtaacagcc
      781 gtcaaagaca tatccttcga cgtacaggag ggagaactct tcgggctgat cggaccggac
      841 ggtgcaggta aatcgactat tttcaggatc cttaccaccc tgctgctggc cgataaaggg
      901 accgcgaccg tagacggact cgatgccgtc aaagatttcc ggcaggtccg gggttgcgta
      961 ggatacatgc ccggccgctt ctccctgtac caggacctca ctgtagccga aaatctgaat
     1021 ttcttcgcaa ccctcttcgg taccagcatc gaaaagaatt acgccctgat cgcggatatc
     1081 tacgaacaga tcaagccatt cagtaccaga cgggccggca agctctcagg tggcatgaag
     1141 cagaagctgg ccctctgctg cgccctcgtc cacaagccca gggtgctgtt cctcgacgaa
     1201 cccacgacag gtgtggacgc cgtgtccagg aaagaattct gggatatgct gaaacggctg
     1261 aaatcacaag ggatcaccat cctcgtctct accccataca tggacgaggc aaacctttgc
     1321 gagcgcattg ccctgatcca ggaaggaagg atactttcca tagattcccc agggaatatc
     1381 gtgaaagcct atcccgataa gttgtatgcc gtcaaggcgg actccatccc ccgcctgctg
     1441 catgacgccc ggttgttccc ggaaaccttg tcctgctatg cctttgggga atacctgcac
     1501 gtatccttca aacccggcga aaccgccaca acacaagcac ctcatcgcat ggaacagtac
     1561 ctgggggaga aaggacatac caatatccgg ctggaagaaa ttgccgccac catcgaagac
     1621 agcttcattc aactgatggg aacacacagc gagccatccg atcacgacaa cccatccgat
     1681 cacaacaacc cgtcgaatca cgatcgttct atcgatccag ccaactcatc caatcttaac
     1741 aaccgctaaa atgaacgaca aagccatcat ctgcgagaac ctgcgaaagc aattcggcga
     1801 tttcatcgcc gtcaatgaca tctctttcga agtgaatgcg ggagagatat acggtttcct
     1861 cggcgccaac ggcgccggca aaaccacggc catgcgcatg ctatgcggac tctcctaccc
     1921 ttccggcggc catgccacgg tagcaggtca caacgtttat aaagagcagg aatccatcaa
     1981 aaagaaaatc ggctacatga gccagaaatt ctccctctac gagaacctca ccatcaggga
     2041 gaacatcgag ttctacggtg gcatatacgg actatcggac aaggacctga aacaaaaagg
     2101 agatgaattg atcaaccgcc tcggactggc caatgaggca agaaaactgg tagg
//
LOCUS       DATGFG010000206         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_557311, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000206 DATGFG010000000
VERSION     DATGFG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557311"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="VKQ52_03755"
     CDS             <1..1711
                     /locus_tag="VKQ52_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86327.1"
                     /translation="LPSQKLIDSLQRVANGDWSKLKGLEQWEKNPGLIQKMVEAREAS
                     MRKKSPDSTSGKSGLAEDKDALDSIYIKKRKEADSIRAEIARVQQLLQSLHTHSQSEV
                     SQGLSGIQRVDNPAALQKELSKLGLTDSALPKGYKTLMAVKSFNIGRTVVNYSELSAK
                     NISVNGVQAEYNPSNYYAFATGSVDYRFRDFIIQQPGQPRQYLNIFRMGRGLKDGNSV
                     ILTYFQGRRQLYNSATSDTSTAQVPSSSLMGLTLEGNYHLTKNILFTGEIAKSTSPSY
                     PGDTAKGEKHGGLFSMGDQSNEAWSTKVTAFFPATQTRIKASYKHLAANYQSFSIFTD
                     GSSQSAWSANVDQLLFKKQLDLMVGANTNDFSNPFIGQEYRSTTVFKSIQATFRRRNW
                     PTVSLGYFPSSQITKLGGSQYIENLFYTLVGNMTESYQFHHLLMNTTVVYTQFYNKAA
                     DSGFTYFNTRNLMVSQSVFLNRFTVQVNAAGATNQDYQLYTLEGKVQHSVNKWLAVGA
                     GVKYNKQTVYNIEQMGYSANATLRLNKLGQIQFSADKGFIPGMDKRLVPDNTGRLTYF
                     KTF"
     gene            1723..2823
                     /locus_tag="VKQ52_03760"
     CDS             1723..2823
                     /locus_tag="VKQ52_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86328.1"
                     /translation="MNKKRVLLAALILISMLSRAQISMTLQVPPIGVLVKNQLWNMLL
                     VNSGNRSLPVSISVVLLDQKTNQPVLTGKTLPFMLDKGAKQIQAKDLGPIQYTYDGPA
                     VLSDRDPNGLLPVGSYQACYTVISGEKSGPRVENCIQLVVDPLSPPLLNTPADEGKTY
                     TAHPQFTWLPPTPAGIFTDLSYALVLVEVMPGQGKADAIEMNIPIYSGGLIRDLYLNY
                     PTSYTDLDTAHLYAWRIVAMNGGLPAAMSDIWTFKVVTPDLSIRGKVNQPYFDLKRGL
                     DPSVASATGSLKLTYDNTPADTVVHYTITSLDDPGNPVIQQGQQPLRRGRNLLELAFS
                     KAAGYAEKKVYLFQLINSRNEAWTLKFTWSAK"
     gene            2859..>3230
                     /locus_tag="VKQ52_03765"
     CDS             2859..>3230
                     /locus_tag="VKQ52_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86329.1"
                     /translation="MIVVLALRFRKHIALFLYVVLYSQLVMAAEVLRAGHGGTGNSPS
                     WEMSDAGYYPAWRELNEIPEVIGDSGVIPARKAAGDVAASPSFRLPATPMAVNKSAKE
                     FIGGPSQPEMSSFQSVNSNNMV"
BASE COUNT          753 a          824 c          943 g          710 t
ORIGIN      
        1 cctgccctcg cagaaactga tcgatagcct gcagcgggta gccaatggcg actggtcgaa
       61 gctaaagggg cttgaacagt gggagaagaa tcccggtctt atccagaaga tggtggaagc
      121 gagagaggcg agtatgcgga agaagagccc ggattctaca tcgggcaaga gcgggctggc
      181 ggaggacaag gatgcgctgg attcgatcta tatcaagaag cgaaaggaag ccgattccat
      241 ccgtgccgag atcgcccgtg tccagcaatt gctgcagtcg ttgcatacgc attcacagtc
      301 ggaagtatcc caggggttgt cgggcatcca acgcgtggac aaccctgccg ccttacagaa
      361 ggaactgtca aaattgggac tcaccgactc tgccttgcca aagggatata agaccctgat
      421 ggctgtcaag tcgttcaata tagggcgaac ggtggtcaat tattcggagc tgtcggcgaa
      481 gaatatctcg gtcaatgggg tgcaggcgga atacaatcct tccaattatt atgcttttgc
      541 cacgggatcg gtggactatc gcttcaggga ttttatcatc cagcagcctg gccagccccg
      601 gcaatacctc aacatcttcc ggatggggcg ggggttaaag gacggcaaca gcgttatcct
      661 gacgtatttc cagggacggc gccaattata taacagcgcc acgagcgaca catctactgc
      721 acaggttcct tcttcttcgc tgatgggcct gaccctggaa gggaattatc atctcacgaa
      781 gaatatcctg ttcaccgggg agatcgcgaa atctacttcg ccgtcgtatc cgggggatac
      841 tgcaaaaggg gagaagcatg gcgggctgtt cagcatgggc gatcaaagta acgaggcctg
      901 gtcgacgaag gtgacggcct tcttccccgc tacccagaca aggatcaagg catcctataa
      961 acaccttgcc gccaattacc agtccttcag catcttcacc gacggctcgt cgcagagtgc
     1021 ctggagcgcc aatgtcgacc agctgctgtt caagaagcag ctcgacctga tggtaggcgc
     1081 caataccaat gatttcagca atccttttat cggtcaggag taccggagta cgacggtgtt
     1141 caagagtatc caggccactt tccgccggag gaactggccg acggtgtcgc ttgggtattt
     1201 ccccagcagc cagatcacga aattgggcgg cagtcaatat atcgagaacc tgttctatac
     1261 gctggtggga aatatgaccg aatcctatca attccatcac ctgctgatga atacgacggt
     1321 cgtctacacc cagttttaca ataaggccgc cgactcgggc ttcacctatt tcaatacgcg
     1381 taacctgatg gtcagccagt cggtgttcct gaacaggttc acggtgcagg tgaatgccgc
     1441 cggcgccacc aaccaggact accagctcta tacactggag gggaaggtgc agcacagtgt
     1501 caacaaatgg ctggcggtgg gcgcgggtgt caaatacaat aagcaaacgg tgtataatat
     1561 cgaacaaatg ggctactcgg cgaatgccac gctgcggctc aacaagcttg ggcagataca
     1621 gttctccgcg gataagggtt ttatcccggg gatggacaaa cggctggtgc cggacaacac
     1681 ggggcgcctt acttatttca aaaccttttg aattgaattt gtatgaataa aaaaagagta
     1741 cttctcgctg cgctgatcct gatctccatg ctctcccggg cgcagatcag catgaccctg
     1801 caagtgccgc ctattggggt gttggtgaag aaccagcttt ggaacatgct gctggtgaat
     1861 tcgggcaacc ggagtctccc ggtgagtatc agcgtggtat tgttggatca gaagaccaac
     1921 cagccggtgt tgacgggcaa gacgctgccg ttcatgctgg acaagggggc gaagcagata
     1981 caggcaaagg atctgggccc gatacagtat acctatgatg gtccggcagt actcagtgac
     2041 cgggacccga acgggttgtt gccggtggga agttaccagg cctgttatac ggtgatctcg
     2101 ggggagaaga gcgggccccg ggtggagaac tgtatccagc tggtggtgga ccctctgagc
     2161 ccgcccttgc tgaatacgcc tgccgacgag gggaagacct acacggcgca tccccagttc
     2221 acctggttgc cgccgacgcc tgcgggtatc ttcaccgacc tcagctatgc attggtgttg
     2281 gtggaggtga tgcccgggca ggggaaggcg gatgccattg aaatgaatat cccgatctat
     2341 agcggcggcc tgatccggga tctgtatttg aattatccca cctcttatac cgatctcgac
     2401 acggcgcatc tctatgcctg gcgtatcgtg gcgatgaatg gcgggttgcc ggcggcgatg
     2461 agtgatatct ggacgtttaa ggtcgtgaca cctgaccttt cgataagggg gaaggtcaac
     2521 cagccgtatt tcgatctgaa gcggggcctc gatccttcgg tggcgtcggc gaccggttcg
     2581 ttaaaactca cgtatgacaa tactccggcg gacacggtag tgcattatac gatcaccagt
     2641 ctggatgatc cgggtaatcc tgttattcaa caggggcagc agccgttgag gcggggccgc
     2701 aatctgctcg agctggcttt ttcgaaggcg gcgggctatg cggagaagaa agtctattta
     2761 tttcaattga tcaatagccg gaacgaggcg tggacgttaa agttcacctg gtcggcaaaa
     2821 taagaaaccc tcttttctct acagcataac gtaatcacat gatagttgtt ttagcgctac
     2881 ggtttagaaa gcacatcgcc ttattcctgt atgttgttct gtattcgcaa ctggttatgg
     2941 ccgcggaggt gctgcgtgcc gggcatggtg gtacgggaaa ttcgccatcg tgggaaatgt
     3001 ccgatgccgg gtattatccg gcctggaggg agctgaacga aatccccgag gtcattggcg
     3061 actccggtgt catacccgcc cgcaaggcgg ccggcgatgt ggctgcttcg ccatccttcc
     3121 ggctgccggc cacgccgatg gctgtgaaca agtcggcaaa agaatttatc ggcggcccca
     3181 gccagcccga gatgtcgtct ttccagtcgg tgaattccaa taatatggtg
//
LOCUS       DATGFG010000207         7482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_539068, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000207 DATGFG010000000
VERSION     DATGFG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539068"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /gene="traJ"
                     /locus_tag="VKQ52_03770"
     CDS             <1..1132
                     /gene="traJ"
                     /locus_tag="VKQ52_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transposon protein TraJ"
                     /protein_id="HLZ86330.1"
                     /translation="ATQAQTAAGGIAQMHVVLANVRQQMMPLVANMITISQSIAGFGT
                     IFYIGFRVWKHIARAEAIDFFPLLRPFVLVLLIGFFQPVVLGTLEGILQPTVTATAQM
                     VDTENAAVMNLISLQARSIGGDTTQQVVMNPYDFGTYRGADQYADPSATTSGSSDSGG
                     FWSSIGSGLKFLMSGMYNTLKVIFQFCLSLILQVLYYAASLCIDTIRTFHLIVLAILG
                     PLAFAFSCYDGLQNSLVHWLARYINIYLWLPIANIFGAILAKIQENMLQIDLANGTSH
                     FVQTEITYLVFLVIGIVGYFTVPGIANYIIHTHGPNPLAQKVSNLAQTAVSVAATAAT
                     GVPVGGAGGGGGTNPTAATGYQDSQGAAKGEANSYSRKQIEG"
     gene            1159..1791
                     /gene="traK"
                     /locus_tag="VKQ52_03775"
     CDS             1159..1791
                     /gene="traK"
                     /locus_tag="VKQ52_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraK"
                     /protein_id="HLZ86331.1"
                     /translation="MFQQLKNIDTAFRHVRLFSILFLFAAVAICCYTIFRTSTALEHS
                     RHEIYLLVNGKLINAIAIDRKDSLSVELRDHVKMFHFYFYSLEPDEQIIKRHLTAALY
                     LADNSAKQEYDNLTETGYYSNIISGNISQQVLDPDSVLVSNNQPPYYFRYYGKLRIVR
                     ETSILTRSLITEGNLRVLSTVSDKNPHGFLIENWKVLENKDLSVELRNNH"
     gene            2181..3488
                     /gene="traM"
                     /locus_tag="VKQ52_03780"
     CDS             2181..3488
                     /gene="traM"
                     /locus_tag="VKQ52_03780"
                     /inference="COORDINATES: protein motif:HMM:NF023922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraM"
                     /protein_id="HLZ86332.1"
                     /translation="MQPQDNKQTKKKRLIAALVIFGLPALLILGVVLSLYHPHSTTET
                     SSGKKDAFNSRMPVPNLKEREKNKLEVYLDAEKDSIRRQQLLHNDPYAKRANSSVPVF
                     DSSEKKVGAARQPAWPGAVSPVDSTERKVNEHLARLYNVLHNATPPGGEGIGPASASQ
                     STLSPYSSAASPLDRSVDRLQQLQNRLQQQDTAPNPQFQQINALLDKVLAIQHPDRSR
                     LASDSVGSSHIVMRNAYPVTTQDPKLANQDTALAAAREFTVDVATGNAFYGFSDDSDT
                     TIPSSQVIRAVVHADQTVQTGSVVKLRLLQDIFIKGVRIPANSFIFGPCAISNERVAI
                     HLSQVQFDGQVYSIDMRVLDGVDALEGLYVPGAISREVLKEGAGQSVSSLGLTSFDPS
                     LGAQAAAAGIETARNLIGRKIRLITATLKAGTLAILQPAQSHP"
     gene            3523..4404
                     /gene="traN"
                     /locus_tag="VKQ52_03785"
     CDS             3523..4404
                     /gene="traN"
                     /locus_tag="VKQ52_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraN"
                     /protein_id="HLZ86333.1"
                     /translation="MKTLISAIIFLSLALTAQLPALGQSLPIPPSTLIVHYRLFISYN
                     STTVIVFPAAVRPVDRGDRDVLAQRQTGVENVLKLKAGRRNFPPTNLHVFTADGKVYA
                     FDLYYTDTLGSTYNLTRLDSSTTQAGPEIVLAGQPLNTEQLNACLDLVRRQSPKYSIR
                     VHRFRMRLQLQNIDMAGPMLFFRFAVSNKSGLDYTPDFLRLYLCDQARAKRTSRQDQE
                     LTPLYADTLETIPGKQTRMLIVGLHRITIPDRKEFRVELYEKNGGRALALRIRNRQLL
                     RALRLTIPTSALYNANQ"
     gene            4391..5476
                     /locus_tag="VKQ52_03790"
     CDS             4391..5476
                     /locus_tag="VKQ52_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86334.1"
                     /translation="MPTNNLPFDRVLDSLLMQAEQEAIQRTRNPTLRREDPYLLGLRQ
                     AQYESILDGYRNRTDLTREEKRGLTYVRTQLRNINARLHPNLFRRIRYLRPVNWLFNW
                     IRGRAALVDGYNNQLQDQDRRTIQEQNLQRLGDEMKKAGFKVIPEATLKRAIAQDRPY
                     FNIPYAASDDAKTDYVLHFKKLPGTDAYYLDNFDAATKPTPQEVLRGNPIVRQNFSLI
                     DDYALSAHEAASVIRHIPVARNIDGQEKWLITDALGERRWVDFDLQAALNRYPIKELR
                     DPARMQALVAALHTGSSRDVTFRKNNQTHELRVQVDSWAEGLLFKDKNGNLVDADRIF
                     QPNSNVQKVLSMAQDEAVVRELHPKRS"
     gene            5487..6065
                     /locus_tag="VKQ52_03795"
     CDS             5487..6065
                     /locus_tag="VKQ52_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86335.1"
                     /translation="MLNLPYTSLADHETLLTEKGWFNPEMGGTRLGDWLKEFREKFEE
                     CRINAVNAGKRMEFSMPATGTFNDSLDKVSFTLRYRYDPRHEALSLITIQTKLGRTPW
                     TYFLNGRREPPTPTEAYEKLRKIEGPLRNDIVLSRVRREHDRQNDPFRDPHLDMNHPG
                     FQQTNDLHRDEEYREHPPNDLTSFFSRPSRRR"
     gene            6119..7117
                     /locus_tag="VKQ52_03800"
     CDS             6119..7117
                     /locus_tag="VKQ52_03800"
                     /inference="COORDINATES: protein motif:HMM:NF018009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HLZ86336.1"
                     /translation="MQLAPTLLQEIKTIWLSARHRAYSQVNRTLIDAYWQIGQRIVLE
                     EQKGEDRARYGAFLLRELAQRLTQTLGKGLDERDLRQMRQFYLCFTDPDVIRPELSWT
                     HYRLLLRVADPAARAYYAKEAAEQGWGTRHLERNIQTGYFSRLLSTHSAPPPDENHKN
                     RFTPADFVKDPFVMEFLGASLPSSFTETDLETALLAQLQRFLLELGNGFAFVGRQFPI
                     RTETKIYYIDLVFYNFILKAFCILELKTGPLTHQDIGQLDMYVRIFEDIKKIPGDNPT
                     IGIILCADKDETLVKYSVLEENRQLFASRYRLVLPSEEELSRQIEKEKEIIHRSKF"
BASE COUNT         1907 a         2247 c         1600 g         1728 t
ORIGIN      
        1 cgccacacaa gcccagacag ccgccggagg catcgcccaa atgcatgtcg tccttgccaa
       61 cgtccgtcag cagatgatgc cgctggttgc caacatgatc accatatccc agtccatagc
      121 tggctttggt accatttttt atattggatt tcgcgtatgg aagcatattg cccgggccga
      181 ggcgattgac ttctttccac tgctcagacc cttcgtcctt gttttgctta tcggcttctt
      241 ccaaccggtc gtgcttggca ccctcgaggg catcctccaa cccactgtca ccgccactgc
      301 ccaaatggtc gataccgaaa acgccgccgt catgaacctc atcagtctgc aagctcgaag
      361 catcggcggt gacactactc aacaagttgt gatgaacccc tacgatttcg gcacctaccg
      421 tggcgccgac caatatgcgg atccttccgc aaccacatcc ggcagctccg acagcggagg
      481 cttctggtcc agtataggaa gtggtctaaa attccttatg agtggtatgt acaatactct
      541 caaagtaatt ttccagttct gtttgtctct tatccttcaa gtcctttatt atgccgcatc
      601 tctctgcatc gataccatca gaacattcca cttgattgtg cttgctattc taggaccgct
      661 agccttcgct ttcagttgct acgatggtct acaaaattcc ctggttcatt ggctggctcg
      721 ctacattaat atatatctat ggctacccat cgccaatatc ttcggtgcaa ttctggctaa
      781 gatccaggaa aacatgcttc agatcgacct cgccaatgga acatctcatt ttgttcagac
      841 ggagattaca tacctagtat tcctggtcat aggcatagtc ggctacttca ccgtcccggg
      901 aatagccaat tatatcattc acactcatgg tcctaatcca ctcgctcaga aggtctccaa
      961 ccttgcccaa acggcagtgt ctgtagctgc taccgccgcg accggcgtcc ctgttggggg
     1021 agccggaggg ggcggaggca cgaaccctac tgctgccacc ggttaccaag atagccaagg
     1081 cgcagccaaa ggggaagcca actcctatag ccggaagcag atcgaaggct aaatcgttca
     1141 cctgatactc taccccctat gttccaacaa ctgaaaaaca ttgacaccgc atttcggcat
     1201 gtccggctgt tctcaatcct gtttctattc gcggccgtgg cgatctgctg ctacaccatc
     1261 tttcgaacct ccacagcgtt ggagcacagc cgtcatgaaa tttatctact tgtaaatggc
     1321 aagctgatca atgctatcgc catcgatcgc aaggattcac tttcagtcga gctcagagac
     1381 catgtaaaaa tgtttcattt ttacttttac tccctcgaac ccgacgaaca gatcatcaaa
     1441 cggcacctta ccgccgccct ctatctcgcg gataatagcg ccaagcaaga gtatgataac
     1501 ctcactgaaa ccggctacta ctccaatatt ataagtggta acattagcca acaagtactt
     1561 gatcccgaca gcgtgctcgt tagcaataat cagccaccct attatttccg gtattatggc
     1621 aagctcagga tcgtccgcga aaccagtatt ctcacccgct cgctcattac tgagggtaat
     1681 ctccgggtcc ttagcactgt cagtgataaa aacccacatg gttttctcat cgaaaattgg
     1741 aaggttttgg agaataaaga cctctccgtt gaactaagaa acaatcatta atctcaccct
     1801 atgcgccttt ttcaccgaaa acgcagacca cccccgccgc cgccgccgcc aaatcaggtc
     1861 acaatggcag ttaaccgctg gatcgatagc aaaaaacgcc gtttggccga tacactcagc
     1921 cggcgtgaac agcggatgac cctcaggcaa aaaaagcggg ctcttgccct gttctgcagc
     1981 atcagtaccg ctctgttcct ttttgaactt tatcacggcg tgtgtttcca cccggcatct
     2041 gcctaccggc aaccagagtt catccatatg ccagccgaac tcccgccccc cgtgccaccc
     2101 caaagcaagc accctcgcac acctggtgac agcactggcc ggatttttcc ttccgattcc
     2161 actcactaaa aagccaacac atgcaaccac aagacaataa acaaacaaaa aaaaagcgac
     2221 tcattgctgc gctcgtcatc tttggactcc cagctttact catcctcggc gtagtcctca
     2281 gcctctacca tccccattcg accacagaga cttccagcgg caaaaaagat gcgtttaact
     2341 cccgaatgcc cgtgcccaac ctcaaagaac gcgaaaaaaa caaactagaa gtttacctcg
     2401 acgcggaaaa agattccatt cgtcgtcaac aattgctaca caatgaccct tatgcaaaaa
     2461 gagccaattc ttcagtcccg gtttttgatt cctctgaaaa gaaagtaggc gccgcccggc
     2521 agccagcttg gcccggagcc gtttctccgg tcgatagcac tgaacgcaaa gtcaacgagc
     2581 acctcgcccg gctctacaat gtcctccaca atgccacccc gcctggagga gaaggtattg
     2641 gtcccgcctc cgccagccag tcgacgctct caccgtactc ctcggcagcc tcccccctcg
     2701 accggtccgt tgaccgactt cagcagctcc agaatcgtct tcagcagcaa gacaccgctc
     2761 caaatcctca gtttcaacaa attaatgccc tacttgataa ggtgcttgcg atccagcatc
     2821 ccgatcgcag tagactggcg tccgacagcg taggatcatc ccacattgtt atgcgcaacg
     2881 cgtaccctgt caccacccaa gaccctaaat tggctaatca agacactgca cttgccgctg
     2941 ccagggagtt cacggtcgac gtcgcaaccg gtaatgcgtt ttatggtttt tctgatgatt
     3001 cagacaccac aatcccttcc tcgcaagtta tccgcgcggt tgtgcatgca gaccaaacag
     3061 tccagaccgg ttcggtagtc aagctccgcc tgcttcaaga tatcttcata aaaggtgtcc
     3121 gcattcccgc caattccttc atctttggac cctgcgcaat cagcaatgag cgcgtcgcca
     3181 tacatctttc ccaggtccag tttgacggtc aggtctactc gattgacatg cgagttttag
     3241 atggggtcga tgccctagaa ggactctacg tacctggcgc aattagccgg gaagtcctca
     3301 aggaaggagc tgggcaaagc gtaagtagcc tcggcctcac ctctttcgat ccctcgctgg
     3361 gcgctcaggc tgctgctgcg gggattgaaa ctgccaggaa cttaataggt cgcaagattc
     3421 gactgataac tgcaaccctt aaggctggca ccctggccat cctccaaccg gcccaatctc
     3481 acccatgatc cccattattt aatcccccca acgtacaaat tcatgaaaac gctcatttcc
     3541 gccatcattt ttctctctct agcgctgaca gcccaactac ccgcacttgg tcagtcctta
     3601 cctatcccgc cttccacttt gatcgtccat taccgattat tcatatccta caattcaacc
     3661 acggttatcg tctttcccgc cgcggtccgg cccgtcgatc gcggcgaccg tgatgttctc
     3721 gcgcaacgac aaaccggggt agagaacgtt ctaaagctaa aagccgggcg tcgcaacttt
     3781 ccgccaacca atctacatgt tttcactgcc gacggaaagg tctatgcttt tgacctttat
     3841 tatactgata ctctcggatc cacgtacaac ctcacccgcc ttgactccag tacgacacag
     3901 gcgggcccgg agattgtgct cgccggtcag cctttaaata cggaacagct taatgcctgc
     3961 ctggaccttg ttcgccggca gtctccgaaa tattcaatcc gcgtccatcg ctttaggatg
     4021 cggctgcaac ttcaaaatat tgacatggcc ggtccgatgc tattctttcg ctttgccgtc
     4081 tccaacaagt ccggtctcga ctacacccca gatttcctac ggctttatct ctgtgaccag
     4141 gccagggcaa agcgaacgtc ccgccaggac caggagctta ctcccctcta tgccgatacc
     4201 ctggaaacga ttcccggtaa gcaaacccgc atgttgattg tcggccttca ccgcataaca
     4261 atccctgacc ggaaggagtt ccgcgtcgaa ctctacgaaa aaaatggggg acgagctctc
     4321 gctcttcgta tccgcaaccg ccagctccta cgtgcccttc gcctgactat ccccacttct
     4381 gcgttataca atgccaacca ataatctacc ttttgatcgt gttcttgatt ccctgcttat
     4441 gcaggcagaa caagaggcca tccagcgcac ccgcaatcca acgctgcgcc gggaagaccc
     4501 ctatttactc ggtctgcgcc aagctcaata tgaatctatc cttgacggtt atagaaatcg
     4561 gaccgacctc acgcgggagg aaaagcgagg ccttacctat gttcgtacac agctccgtaa
     4621 cataaatgcc cgcttacacc ccaatttgtt tagacgcatt cgctacttgc gtcctgttaa
     4681 ctggttattc aattggataa gaggacgcgc cgctctcgta gacggatata acaatcagct
     4741 acaggaccag gatcggcgaa cgatccaaga gcaaaacctc cagcgactcg gcgacgaaat
     4801 gaaaaaggcc ggtttcaaag taatcccgga ggcgaccctg aaaagggcca ttgcccagga
     4861 ccggccatat ttcaatatcc catacgcagc ttccgacgac gccaagactg actatgtgtt
     4921 acactttaag aaactgcccg gcacggatgc ttactatctg gacaatttcg atgctgccac
     4981 taagcccact ccccaagaag ttctcagagg gaatcccatc gtccggcaaa acttttcact
     5041 catcgatgat tatgcgctat ccgcccacga agcggccagc gtcatcagac acatacccgt
     5101 cgcaagaaat atcgacggcc aagaaaaatg gcttatcacc gatgcgctgg gcgaaaggag
     5161 atgggtagac ttcgacctgc aggccgctct caacagatat ccgataaaag agctacgaga
     5221 ccctgctaga atgcaggcgt tagtggccgc gcttcacacc ggtagctcac gtgacgtcac
     5281 attccgtaaa aataaccaga cccatgagct gcgggtacag gtagactcct gggcggaagg
     5341 cctgctcttc aaggacaaga atgggaacct cgtcgatgcg gaccggattt tccagccaaa
     5401 ctccaacgta caaaaagtcc tttcaatggc tcaagacgag gccgtggttc gagaacttca
     5461 cccgaaaaga tcctaaaaaa gcagtcatgc tcaaccttcc atatacctct ctcgctgatc
     5521 acgaaactct cctcacagag aaaggctggt tcaacccgga gatgggcggt acccgcctgg
     5581 gagactggct caaggaattc cgggaaaaat tcgaagaatg ccgtatcaat gctgtcaatg
     5641 ccggcaaaag aatggaattc tcgatgccgg caaccggcac attcaacgat agcctcgata
     5701 aagtgtcctt caccctccgt tatcggtatg acccccgcca cgaagccctt tcattaatca
     5761 ctatccaaac aaagcttgga cgtaccccat ggacttattt cctcaacggc cgcagagaac
     5821 cgccaacgcc gaccgaagcc tatgagaagc tccggaaaat tgaaggcccg cttcgaaacg
     5881 atatagtcct ttcccgcgtt cgacgcgagc acgatcggca aaacgatcca ttccgcgacc
     5941 cccacctcga catgaatcac cccggattcc agcaaacgaa tgatctccat agagatgagg
     6001 aatatcgcga gcacccgcca aatgatctta cttccttctt cagtcggcca tcccgtcgcc
     6061 gataattcgg gacgcactgc gtcccgaatt ttactttcca acaaatcgat tcattatcat
     6121 gcaattagct ccgacccttc tccaagaaat caaaaccatc tggctttctg cccggcatag
     6181 agcgtattcc caggtcaacc gtacgctcat cgatgcctat tggcagatcg gacaacggat
     6241 cgtgctggaa gaacagaaag gcgaggatcg cgccaggtat ggagccttcc tcctccgcga
     6301 gctggctcaa cggctcaccc agacacttgg caaaggcctc gacgaacgag atctccggca
     6361 aatgcgtcag ttctatcttt gtttcaccga cccggacgta atccgcccgg agctcagttg
     6421 gacccattat cgccttcttc tcagggtggc ggatcctgcc gcacgcgcct attatgcaaa
     6481 agaggccgcc gaacaagggt ggggaacccg ccatctcgaa cgaaacatcc agaccggcta
     6541 ctttagccgc ctgctctcca cacattccgc tccaccaccg gatgaaaacc ataaaaatcg
     6601 tttcaccccc gcagactttg tgaaggatcc gtttgtcatg gagttcctcg gtgcctccct
     6661 gccttccagc ttcaccgaaa ccgacctcga gactgccctc cttgctcagc tccagcggtt
     6721 ccttcttgaa ctcgggaacg gattcgcctt tgtcggccga caattcccca tccgcaccga
     6781 gaccaagata tattacattg acttggtatt ttacaacttc attcttaagg cattctgcat
     6841 tctggaacta aagacgggcc cactaacgca tcaggacata ggccaactcg atatgtatgt
     6901 acgaattttc gaagatatca agaaaattcc tggcgataac cccacgattg gtattatcct
     6961 ctgcgccgat aaagatgaaa cccttgtcaa atacagtgtc ctcgaagaga accgccaact
     7021 ctttgcttct cggtaccgct tggtcctacc ctccgaagag gagctcagtc gtcaaatcga
     7081 aaaagaaaag gagattatcc accgatccaa attctaatga ttggcatgtg ccattttcca
     7141 acttttcacc ggcatacatc actgtagtag gtccggcaat atggtttcag taaaacacca
     7201 agccggagac tgcaatttta tctcagtatc aaggagccac cagatcggtg gcttttgttt
     7261 tataagctat ggtagagaat tccaagcagg ggagacgcac atacgcaaaa aataaaacta
     7321 atgcaacgtg aaaaacacgg tatttttttg ggagggaatg aacattgggt gtctggcggg
     7381 ttccagtttg gcagttatat cggggaaaca atacatctgt aaatcaaaca tgtagtaatt
     7441 tcgcgagact taattcatta taaccgttta taattcatga gc
//
LOCUS       DATGFG010000208         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_538805, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000208 DATGFG010000000
VERSION     DATGFG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538805"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1504
                     /locus_tag="VKQ52_03805"
     CDS             176..1504
                     /locus_tag="VKQ52_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLZ86337.1"
                     /translation="MALFQQILFVLALAAATGLFVKKIRQIRRNILLGKEESFSDNPG
                     LRWKNLLLLALGQKKMFRNPLVAFLHLLIYAGFIIINIEVLEIVLDGILGTHRVFAGF
                     GGALYSVLIDAFEFLAVGVFFACFIFLCRRNILKLRRFISHDLDGWPRSDANYILLTE
                     IVLMTLFLTMNAADTLLQARGYGHYGARLTGDFAFSRWLHPLLNGLSSPGLVVLERVC
                     WWLHILGILAFLNYLPYSKHLHILLAFPNAYYARLEPEGKLRNMPVIQQEVLYAMQPE
                     TVPPAPADGTAPPPPRFGAKDVFDLSWRNLLDAYTCTECGRCSAACPANTTGKLLSPR
                     KIMMSTRDRLEEVGRNIDANGSFTDDGKSLLNYISVEELRACTTCNACVTECPVSISP
                     LEIIVEMRRALIMEDSNAPQEWNALFGNIENNFAPWKFSPEDRDKWATEL"
     gene            complement(1563..2102)
                     /locus_tag="VKQ52_03810"
     CDS             complement(1563..2102)
                     /locus_tag="VKQ52_03810"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ86338.1"
                     /translation="MKNLIGILSAVLVSITGIAQEYKPVDQGSLVEFVIKNLGFNTKG
                     SFTGLDGRISFDPANAGKDSFDVSIDAATVNTDNSMRDGHLKKESYFDVEKYPRIRLV
                     STSVTGPDRSGHYTFNGKLTIRDKTKDISFPFIVTPMAEDYIFKGSFTISRRDFEVGG
                     SSTISNNLTINLTVLAKKQ"
     gene            complement(2099..>2576)
                     /locus_tag="VKQ52_03815"
     CDS             complement(2099..>2576)
                     /locus_tag="VKQ52_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ86339.1"
                     /translation="EIIQKGELFGQFSLERSNLHGEFAQAYKNNVSICAFTIDDFERL
                     LKNRPDLALKYSKQVGAKLRGIENRLVNLLNKDVKTRLIHFLWQLVEQNLGENTAEGF
                     CIPNFLTHEDIASLIGSSRQTVTTMINELETEGILSYDRQEICFLDVKKLQKRIQA"
BASE COUNT          515 a          715 c          702 g          644 t
ORIGIN      
        1 gttgttgttc gccggcgtta ttctccagga tctcataagc catgcggcgc agcttctttt
       61 ctgccacggg ttgcgtaagg atgtaatttt tcattccggc taccaattaa ggattgcggc
      121 aaaaataggc tttagaccaa atcacttatc ttagtcccat caataaattt tcattatggc
      181 cctttttcag cagatcctgt tcgtcctggc cctcgcggcg gccaccggcc tgttcgtgaa
      241 aaagataaga cagatccgcc ggaacatcct gttgggaaag gaggaatcct tttccgacaa
      301 ccccggtctc cgatggaaga acctcctgct gctggccctc gggcagaaga agatgttccg
      361 taacccgctg gtggcctttc tgcacctgct gatctatgcc gggtttatca tcatcaatat
      421 tgaagtgctg gagatcgttc tcgacggcat cctggggacg caccgcgtct ttgccgggtt
      481 tggcggggcc ctgtactccg tcctgatcga cgccttcgag ttcctggccg tgggtgtctt
      541 tttcgcctgt ttcatctttc tctgccggcg gaatatcctg aaactgcgcc gttttatcag
      601 ccatgacctg gatggctggc cccgcagcga tgccaattac atcctgttga cggagatcgt
      661 gctgatgacg ttgttcctga cgatgaatgc cgccgacacc ctgttgcagg cccgcggtta
      721 tgggcattac ggcgcccggc tgacgggtga tttcgccttt tcgcggtggc tgcatcccct
      781 gctgaacggt cttagcagcc cggggctggt ggtgctcgaa cgggtgtgct ggtggctgca
      841 tatcctgggc atcctggcct ttctgaacta tctcccctac tccaagcacc tccatatcct
      901 cctggccttc cctaatgcgt attatgcaag gctcgaacct gagggaaaac tgcggaatat
      961 gccggttatt caacaggaag ttctatacgc tatgcagcca gaaaccgtac caccggcgcc
     1021 cgcggatgga acggcgcctc ccccaccccg cttcggcgca aaagatgttt ttgaccttag
     1081 ctggcgcaac ctgctcgatg cctatacctg tacggaatgc ggccgctgct ctgcggcctg
     1141 ccccgccaat acgacgggca aactcctgtc cccccgcaag atcatgatga gtacccgcga
     1201 tcggctggag gaggtgggcc ggaacatcga cgccaacgga agttttaccg atgacggcaa
     1261 gtccctgctc aactatatct cggtggagga attgcgggcc tgtactacct gcaatgcctg
     1321 tgtaacggag tgccccgtga gcatcagccc gctggagatc atcgtcgaga tgcggcgcgc
     1381 cctcatcatg gaagacagca acgccccgca ggaatggaat gcccttttcg gcaatatcga
     1441 gaacaatttc gcgccatgga aattcagccc tgaagatcgg gacaagtggg ctaccgaact
     1501 gtagccgccg cccgcggaac ccggatagcg ggccgcaccc ggtgcgcggt tgaagggagg
     1561 cgttattgct ttttcgccag gacggtgagg ttgatggtaa gattattgga gatggtgctg
     1621 gacccgccga cctcgaaatc ccgccggctg atcgtaaaac tccccttgaa gatatagtcc
     1681 tcggccatcg gggtcacgat gaacgggaaa gagatatcct ttgtcttatc cctgatcgtc
     1741 agctttccgt tgaaggtata atgaccgctt ctgtcgggac cggtcaccga ggtcgagacc
     1801 agcctgatcc gggggtattt ctccacgtcg aaataggatt ctttcttcag gtggccatcg
     1861 cgcatactgt tatccgtatt gaccgttgcc gcgtcgatgc tgacatcgaa actatccttg
     1921 ccggcattcg ccggatcgaa gctgatccgg ccatcgagac cggtgaaaga accctttgta
     1981 ttaaagccga gattctttat cacaaattcc accaatgacc cctggtcgac tggcttatac
     2041 tcctgtgcta tgccggtgat ggaaaccagc accgccgata aaattccgat gagattcttc
     2101 atgcctgaat gcgcttttgt aattttttta catccaggaa acagatctcc tggcgatcgt
     2161 atgagaggat gccttcggtc tcgagttcat tgatcatggt cgtgaccgtc tggcggctgc
     2221 tgccgatgag actggcgatg tcttcgtgtg taaggaaatt ggggatacag aacccttcgg
     2281 cggtgttctc gccgaggttt tgctctacca actgccagag gaaatgtatg aggcgtgtct
     2341 ttacatcttt attgagcagg ttgaccaggc ggttctcgat gccccggagt ttggcgccca
     2401 cctgtttgct gtatttgagg gccaggtcgg gccggttctt cagcaggcgt tcgaaatcgt
     2461 cgatggtaaa agcgcagatg cttacattat tcttatatgc ctgggcgaat tccccgtgga
     2521 gattgctccg ttcgaggctg aactgcccga agagttcccc tttctggatg atctcc
//
LOCUS       DATGFG010000209         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1448544, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000209 DATGFG010000000
VERSION     DATGFG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448544"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2123)
                     /locus_tag="VKQ52_03820"
     CDS             complement(<1..2123)
                     /locus_tag="VKQ52_03820"
                     /inference="COORDINATES: protein motif:HMM:NF023853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HLZ86340.1"
                     /translation="MSSLRFKALDNLATADGAHIPGAAKITAIFGENVFTLKTAREFL
                     SDEAYKSLLASIKGGKKIERAVANQIASGMRQWAEAKGVTHFTHWFQPLTGTTAEKHD
                     SFFTIKSDGTPIEEFDGAALIQQEPDASSFPSGGLRATFEARGYTAWDPSSPAFVMEV
                     GAGKTFCLPTIFVSYTGESLDYKAPLLKALAALDKAAVEVCHYFDKNVNKVTATLGWE
                     QEYFVVDEGLFNARPDLVMSGRTVFGHAPAKGQQLEDHYFGSIPERVYAFMRDYEQEA
                     YKLGIPLRTRHNEVAPAQYECAPIFEEVSVAVDHNILLMDVMDRVARRHKLRVLLHEK
                     PFAGINGSGKHNNWSMATDTGVNLLAPGKTPKTNLMFLTFFVNTIKAVYDYSDVLRAS
                     IAGAGNDFRLGANEAPPAIISVFIGEYLSKVLADVKERVGDKFDEQDEAILKLDLHRS
                     IPELLLDNTDRNRTSPFAFTGNKFEFRAVGSSANCANAMIAVNTILAETLKKFKKDVD
                     ALIEKGDKKEIAIMHVIREYVVSSEKVLFEGDGYSDEWHHEAERRGLPNVRTTPLALD
                     AMVTEKAKHLYESNGVYSHVELEARHEIELEKYIKRVQIEARIMGELATSHVLPPAIK
                     YQNTLIQNISGLKALGLPTAAYSNQQQILEKISQHIGKVSEMVEKMIEARKVANNITD
                     TRTKAIAYNSQVKEPFFDQIRYNVD"
BASE COUNT          465 a          532 c          742 g          602 t
ORIGIN      
        1 ttgtccacgt tgtagcggat ctggtcgaag aagggttctt ttacctggct gttgtaggcg
       61 atcgcttttg tacgggtgtc ggtgatgttg ttggcgacct tccgggcttc gatcatcttt
      121 tccaccattt cgctgacctt gccgatgtgc tgggagatct tttcgaggat ctgctgctgg
      181 ttggagtagg cggccgtggg cagaccgagg gctttcaatc cgctgatgtt ctggatcagg
      241 gtgttctgat atttgattgc cgggggaaga acgtggctgg tggccagttc gcccatgatc
      301 cgggcttcga tctgaacacg tttgatatat ttttccagtt cgatctcgtg gcgggcttcc
      361 aattcgacat gggagtaaac gccgttggat tcgtacaggt gtttggcctt ttcggtgacc
      421 atggcgtcga gggccagcgg agtggtccgg acgttgggca gaccgcgtct ttcggcttca
      481 tggtgccatt cgtcgctata gccgtcgcct tcgaagagga ccttttcgct gctgacgacg
      541 tattcgcgga tcacgtgcat gatggcgatc tcttttttgt cgcctttttc gatgagggca
      601 tccacatcct ttttgaactt cttcagcgtt tcggcgagga tcgtgttaac cgcgatcatg
      661 gcgttggcgc agttggcgct ggagcctacg gcacggaact cgaatttgtt gccggtgaag
      721 gcgaaggggg atgttctgtt gcgatcggta ttgtccaaga gcagttcggg gatgcttctg
      781 tgcaggtcga gcttgaggat ggcttcgtcc tgttcgtcga acttgtcacc tacgcgttct
      841 tttacatccg cgagaacctt gctgaggtat tcgccgatga agacggagat gatcgcggga
      901 ggcgcttcgt tggcacccag gcggaaatcg ttgccggcgc cggcgatgga agcgcgcagt
      961 acatcggagt agtcatatac ggccttgatg gtattgacga agaaggtcag gaacatcagg
     1021 tttgtcttcg gggtcttacc gggagcgagg aggttgacac cggtgtcggt ggccatgctc
     1081 cagttgttgt gcttaccgct gccattgatg ccggcgaagg gtttttcatg gagcagcacg
     1141 cgcagtttgt ggcggcgggc gacgcggtcc atgacgtcca tcaggaggat gttgtggtcg
     1201 acggctacgc ttacttcttc gaagatggga gcgcactcgt actgggcggg agcgacttcg
     1261 ttgtgacggg tacggagggg aatgcccagt ttgtaggctt cctgctcgta gtcacgcatg
     1321 aaggcgtaga cgcgctcggg gatgctgccg aagtagtggt cttccaactg ttgtcctttt
     1381 gcgggggcgt ggccgaagac ggtgcgaccg ctcatcacca ggtcgggccg ggcattgaac
     1441 aggccttcgt cgaccacgaa gtattcctgc tcccatccca gggtggcggt gaccttgttg
     1501 acgttcttgt cgaagtaatg gcaaacctct acggcggcct tgtcgagggc ggccagggcc
     1561 tttaacaacg gtgctttata gtccagggat tcaccggtat aggaaacgaa gatggtggga
     1621 aggcagaagg ttttgccggc acctacttcc ataacaaagg caggagatga ggggtcccat
     1681 gcagtgtagc cacgggcttc gaaagtagcg cgcagaccgc cgctggggaa agaggaggcg
     1741 tcgggttcct gttggatcag ggccgcgccg tcgaattctt cgatgggagt gccatcgctt
     1801 tttatcgtaa agaatgaatc atgcttttca gccgtggtcc cggtgagggg ttggaaccag
     1861 tgggtaaagt gagtgacccc ttttgcctca gcccattgac gcatgccgga tgcgatctgg
     1921 ttggctaccg cgcgttcgat cttcttgccg cctttaatag aagccaggag gcttttatag
     1981 gcttcatcac ttaaaaattc ccgtgccgtt ttcagggtga aaacattctc gccgaagatg
     2041 gcggttattt tggccgcccc ggggatatgg gcaccgtcag cagtggctag attgtcaaga
     2101 gctttgaatc ttaatgagga catgcttgaa aagttttagc gaaactacaa tgattgaagg
     2161 atataacaca aatttttgat tccagggggc caaaaatcca atattgacaa taaaaaagat
     2221 ttatattaaa taattcataa tctgtgcgat aagcagcttt ccaggttctc attaaggcgt
     2281 tgcaggaagg gcagggtgcg gagggaatgc aggaaggggg ccaggaggaa aaggagataa
     2341 a
//
LOCUS       DATGFG010000210         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_538639, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000210 DATGFG010000000
VERSION     DATGFG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538639"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VKQ52_03825"
     CDS             <1..229
                     /locus_tag="VKQ52_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86341.1"
                     /translation="EWLIIYSREVLQKEHFDYFIFGHRHLPIDFLLDETGSRYINLGD
                     WIRYYTYAVFDGQQLSLLSQTPETADKIVRK"
     gene            226..1089
                     /locus_tag="VKQ52_03830"
     CDS             226..1089
                     /locus_tag="VKQ52_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86342.1"
                     /translation="MRSVSGYTVLFLVSLAAGRPAEAQQGAEQRAAAQQAADTGSAFR
                     LTRTLGPDIVDFTVDNLGNIYVLNKDNRLKKLGPQGDSLAVFNDVRRFGQIASIDVSN
                     PLKILVYYREFATIIELDRFLNIVNTIDLRKLNILQAKAVGLAYDNNIWVFDELDAIL
                     KRVGDDGSLVDQTTDFRQLFDSIPDPTVIRDQGGLVYLYDSAKGVYIFDHYGTLKTHL
                     DLRGWRDFDVIDKNLLGRDDRQFYRYQPGTLNMQEEPIPPAYAGASRIRITPTIIYVL
                     KRTQLEIYSRK"
     gene            1136..2245
                     /locus_tag="VKQ52_03835"
     CDS             1136..2245
                     /locus_tag="VKQ52_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HLZ86343.1"
                     /translation="MHSFWNIPVFGNPLKAYILVAATILFVIVLKRYISRLIARLIFR
                     FIRRMGSGLDRSAFLDLVVGPIGTFLVVFVSLTSIEKLHFPSELEFDIYEVRSKAITQ
                     SVAVIILIGSFIWLLLRLVDFVALILRNKARAGGGPRDNQMIFFIRDFLKAVLGIIGI
                     LMILHSAFAVDVSAVVGGLGLAGAAIALAAKESIENLIASFVIFFDKPFKAGDVLKVN
                     NISGTVEKIGLRSTRIRTDQKTFVTVPNKQMVDSVVDNLTLRTQRKGELRLELSLSTP
                     ASQFDAFIAGIRQILVKDKVENSTVFLDNISGSACLVNVDYFTSPMAIEQFNAIKQQV
                     NLDVLRLMEEMELSIAGSSTNITVTNPGPPEPVKP"
BASE COUNT          489 a          695 c          758 g          582 t
ORIGIN      
        1 ggaatggctg atcatttaca gccgggaagt gctgcagaag gagcatttcg actattttat
       61 tttcgggcat cgtcatttgc cgatagactt tttactggat gaaaccggca gccggtatat
      121 caatctgggt gactggattc gttattacac ctacgccgta ttcgacggac agcagctctc
      181 cctgctgagc caaacccccg aaacggccga taaaatcgtc cgcaaatgag atcggtgtcc
      241 ggctataccg tcctgttcct ggtttccctg gccgcggggc ggccggcgga ggcgcaacag
      301 ggggcggagc aacgggctgc ggcgcaacag gcggcggaca cgggttcggc cttccggctg
      361 acccgcaccc tggggcccga tatcgtcgat ttcaccgtcg acaacctggg caatatctat
      421 gtcctgaaca aggataaccg gctgaagaag ctcgggccgc agggtgattc cctcgcggtc
      481 ttcaatgatg tccgccgttt cgggcagatc gccagcatcg atgtctccaa cccgctcaag
      541 atcctggtct attaccgcga gttcgcgacc attatcgagc tggaccgctt cctcaacatc
      601 gtcaatacca tcgatctccg taaactgaat atcctgcagg caaaggccgt cgggctggcc
      661 tatgacaaca atatctgggt gttcgacgag ctcgacgcca tcctcaaacg tgtcggcgac
      721 gatggatcgc tggtggatca gaccacggac ttccggcagc tgttcgattc catccccgac
      781 cctaccgtca tccgtgacca gggtggtctt gtgtatctct acgactccgc caagggcgtt
      841 tatatctttg atcattatgg tacgctaaag acccatctcg acctgcgcgg ctggcgggat
      901 ttcgatgtca tcgacaagaa cctgcttggc cgggatgatc ggcagttcta ccggtaccag
      961 cccggcaccc tgaacatgca ggaagagccc atcccgcccg cctatgccgg cgcgagccgg
     1021 atccggatca ccccgacgat catctatgtg ctgaagagaa cgcagctgga gatatatagc
     1081 cgaaaatgat cgtgccgggg gcgaggccag acgatcgact ccctatcttt gtaccatgca
     1141 cagcttctgg aacatcccgg tctttggcaa tccgctgaaa gcatatatcc tggtcgccgc
     1201 caccatcctt ttcgtcatcg tcctgaagcg gtatatctcc cggctgatcg cgcggctgat
     1261 ctttcgcttt atccggcgga tggggtcggg cctggacaga tcggctttcc tcgatcttgt
     1321 cgtagggccg atcgggacgt tcctggtggt ttttgtgtcg cttacctcga tcgaaaaact
     1381 gcatttccct tccgagctgg agttcgatat ctatgaggtg agatcgaagg ccatcaccca
     1441 gtcggtggcg gtgatcatcc tgatcgggag ctttatctgg ctcctgctgc ggctggtgga
     1501 ttttgtcgcc ctgatcttgc ggaacaaggc aagggccggc gggggtccga gggacaacca
     1561 gatgatcttc tttatcaggg acttcctgaa ggccgttctg gggatcatcg gcatcctgat
     1621 gatcctgcat tcggcgtttg ccgtggatgt ttcggccgtt gtcggtggcc tggggctggc
     1681 cggcgcggcg atcgcccttg ccgcaaagga aagtatcgaa aacctgatcg cctcctttgt
     1741 gatcttcttt gacaagccct ttaaggccgg ggatgtatta aaggtgaaca atatctccgg
     1801 tacggtggag aagatcgggc tgcgcagtac ccgcatccgg acggatcaga agacctttgt
     1861 caccgttccc aataaacaga tggtggacag tgtcgtcgac aacctcaccc tgaggaccca
     1921 gcgaaaaggg gagttgcggc tggagctgag cctttcgacg ccggcgtcgc agttcgacgc
     1981 gtttatcgcc ggtatccggc agatcctggt aaaggacaag gtggagaaca gcacggtttt
     2041 cctggataac atctcgggca gcgcctgcct ggtcaacgtg gactatttta cttccccgat
     2101 ggccatcgaa cagttcaatg ccataaaaca gcaggtaaat ctcgatgtgt tgcggttgat
     2161 ggaggagatg gagcttagta ttgccggttc gagtacgaat attacggtga ccaacccggg
     2221 gccgcccgag ccggtcaagc cctgaggtgt cagtggggcc ggccggcgga ggttgggcgt
     2281 tggagtccgg ccgcaagggt tcggcgttgc gggagccgcg ggttgccgga gtcgggaggg
     2341 gcgtccgcgg gagccggggg tcgccggagc cgggatgggc gtcagagttg cgccttccat
     2401 tccacgagga aggcctttat ttgttggagg cgttggatga cctcgaagcg ctcttcggct
     2461 ttgctgttct ttttcaggaa ttctttcgcg ccctcgatgg tgtatttttt ttgccgcagg
     2521 aggt
//
LOCUS       DATGFG010000211         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_762990, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000211 DATGFG010000000
VERSION     DATGFG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762990"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="prfB"
                     /locus_tag="VKQ52_03840"
     CDS             <1..889
                     /gene="prfB"
                     /locus_tag="VKQ52_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HLZ86344.1"
                     /translation="DTAVEDFAVLFDFWKAGEATEEETREAYDKALAQIEEVEFKSTL
                     NQPEDELPAVMQINSGAGGTESQDWAEMLLRMYRMYGEKQGWSVTELDLQDGEGAGIK
                     SATIQFDGPFAYGFLKAESGVHRLVRISPFDSNARRHTSFASVFVYPLVDDSIEIEVS
                     PADLEWEFYRSGGKGGQNVNKVETAVRLKHIPSGIIVECQQARTQGENREKALKMLKS
                     RLYEEELRKREAMKNATNSTKKKIEWGSQIRSYVFHPYKMIKDHRTDYEVGNVQPVMD
                     GELDGFIKAYLMNEKETTT"
     gene            905..2536
                     /gene="pruA"
                     /locus_tag="VKQ52_03845"
     CDS             905..2536
                     /gene="pruA"
                     /locus_tag="VKQ52_03845"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HLZ86345.1"
                     /translation="MSLGYFHYPAPANEPVLHYAPGSKEREALKKTLKELKSTEHDIP
                     MYIGDKEVRTGKKIALRPPHEIAHTLGHFHEGDETHVQQAIDAALKAKAKWAALSWED
                     RAHIFLKAADLISTKYRPYMNGTTMLGQSKNAYQAEIDSACELIDFLRWNVHFLSEIY
                     RQQPVSSPGIHNRTEWRPLEGFVLAITPFNFTAIGGNLPTSAALCGNTVVWKPAYTQV
                     FSAQMFMRILREAGLPDGVINLIYVDGPLLGKVCFGHKDFAGIHFTGSTAAFNHIYKT
                     IGENISHYRSYPRIVGETGGKDFVMAHPSADPDVLATALLRGAFEYQGQKCSAASRAY
                     IPENLAAAVKQKLVDGIKSFKMGPVEDFSNFVNAVIDERSFNKITSYLDKVKQDPAKA
                     TILAGGNGSKAKGYFIEPTVIEAKDPKFVTMCEEIFGPVLTIYVYPTAEFEKTLDLVD
                     STSPYALTGSIIAQDRTAIDLATNRLRNAAGNFYINDKPTGAVVGQQPFGGARGSGTN
                     DKAGSMLNLYRWLSARTIKETFVPPTDYHYPFLAE"
     gene            complement(2615..3061)
                     /locus_tag="VKQ52_03850"
     CDS             complement(2615..3061)
                     /locus_tag="VKQ52_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86346.1"
                     /translation="MITVSDLNCKEENYTWWLNIMPDEMPRLSILPKKVRARLDYSPA
                     SLDVIEEYIRENYTTEEMKDRKNKFARDLFARYVGETLRKNVPGLYWSFESEDDQHPH
                     YGVPVLLTLGEEDAVPPMTPTLWVMTLIEQEGNFLRGQLRPAKKAA"
     gene            3436..4032
                     /locus_tag="VKQ52_03855"
     CDS             3436..4032
                     /locus_tag="VKQ52_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HLZ86347.1"
                     /translation="MKKLALVLFAGISFATAHAQVQFGVKAGANFATQTGSDAGDSKT
                     LFNFNAGAFLRLAVVQGFSIQPELVYSGQGASYDVSGGTLREHANYLNIPVLFKWSHR
                     AGPYFETGPQLGFILSAKASINGNSTDSKSAYNSTDFAWVFGVGYKIPRSPVGIDLRY
                     NIGIANVEDRGVTGQTGSWRNDVVQLGLTYVLFNGPRR"
BASE COUNT         1092 a         1330 c          973 g          819 t
ORIGIN      
        1 cgacacggca gtggaagact ttgccgtatt gttcgacttc tggaaggccg gcgaagccac
       61 cgaagaagag acccgcgaag cctacgacaa agcgctcgcc cagatcgaag aagtagagtt
      121 caaaagcacg ctcaaccagc cggaagacga actcccggcc gtcatgcaga tcaactccgg
      181 cgccggcggc accgaaagcc aggactgggc cgaaatgctc ctgcgtatgt accgcatgta
      241 cggggaaaaa cagggctggt ccgttaccga actcgatctc caggacgggg aaggcgccgg
      301 gatcaaaagc gccaccatcc agttcgacgg ccccttcgcc tatggcttcc tgaaagccga
      361 aagcggggtc catcgcctcg tccgcatctc ccccttcgac tccaacgccc gcaggcatac
      421 ctccttcgcc tcggtcttcg tctaccccct cgtcgacgac agcatcgaga tcgaggtcag
      481 ccccgccgac ctggaatggg agttctatcg cagcggcggt aaaggcggcc agaacgtgaa
      541 caaggtcgaa accgccgtcc ggttgaaaca tatccccagc ggcatcatcg tcgaatgcca
      601 gcaggcccgt acccagggcg agaaccgcga aaaagccctg aaaatgctga aaagccggct
      661 ctatgaagaa gaactgcgca aacgcgaggc gatgaagaac gccaccaact ctacaaaaaa
      721 gaagatcgaa tggggctccc agatccgcag ctacgtcttc cacccgtaca agatgataaa
      781 agaccatcgc accgactacg aagtaggtaa cgtacaaccg gtgatggacg gcgaactcga
      841 cggcttcata aaagcctacc tcatgaacga aaaggaaacg accacctaaa aaacgataca
      901 cccaatgagc ttaggctatt tccattaccc ggctccggcc aacgaaccgg tcctgcacta
      961 cgccccgggc agcaaagaaa gagaggccct caaaaaaaca ctgaaggagc tgaagagcac
     1021 cgaacacgat atccccatgt acatcggcga taaagaggtc cgcaccggca aaaagatcgc
     1081 cctgcgcccc cctcacgaga tcgcccatac cctgggacat ttccacgaag gcgacgaaac
     1141 ccacgtccaa caggccatcg acgcggcact gaaggcaaag gcaaaatggg ccgccctctc
     1201 ctgggaagac cgcgcccata tcttcctcaa agccgccgac ctcatctcga caaagtaccg
     1261 tccctatatg aacggtacca ccatgctcgg ccagagcaag aacgcctacc aggccgagat
     1321 cgacagcgcc tgcgaactca tcgacttcct ccgctggaac gtccacttcc tcagcgagat
     1381 ctaccgccag cagcccgtga gcagccccgg catacataat cgtaccgagt ggcgccccct
     1441 cgaaggcttc gtcctggcca tcaccccctt caacttcacc gccatcggcg gcaacctgcc
     1501 cacctccgca gccctctgcg gcaacaccgt cgtctggaaa cccgcctaca cccaggtctt
     1561 ctccgcacag atgtttatgc gcatcctcag ggaagccggc ctgcccgacg gcgttatcaa
     1621 cctgatctat gtcgacggcc ccctgctcgg aaaggtctgc ttcggtcaca aagacttcgc
     1681 cggcatccac ttcaccggca gcaccgccgc cttcaaccat atctataaaa ccatcggcga
     1741 gaacatcagc cactaccgca gctacccccg catcgtcggt gaaacgggcg gcaaagactt
     1801 cgtaatggcc catccaagcg ccgacccgga cgtcctggcc acggccctgc tccgcggcgc
     1861 cttcgaatac cagggacaaa aatgctccgc cgcctcccgc gcctatatcc ccgaaaacct
     1921 cgccgccgcc gtcaaacaaa agctggtcga cggtataaaa agctttaaaa tggggcccgt
     1981 cgaagacttc tccaacttcg tcaacgccgt catcgacgaa cggagcttca acaagatcac
     2041 ctcctatctc gataaggtaa aacaggaccc cgcaaaagcc accatcctcg ccggcggcaa
     2101 cggcagcaaa gcaaagggct attttatcga accgaccgtc atcgaagcaa aagacccgaa
     2161 gttcgtgacc atgtgtgaag agattttcgg accggtactg accatttacg tctaccccac
     2221 cgccgaattc gaaaagaccc tcgacctcgt cgactcgaca tcgccctatg ccctcaccgg
     2281 ctccatcatc gcgcaggacc gtaccgccat cgatctggcg acgaaccgcc tccgcaacgc
     2341 cgccggtaac ttttatatca acgataaacc caccggcgcg gtcgtgggcc aacaaccctt
     2401 tggcggcgcc cgcggctccg gaaccaacga taaggccggc tccatgctca acctctatcg
     2461 ctggctaagc gcaaggacca taaaggagac ctttgttccg ccaaccgact accactatcc
     2521 cttcctcgcc gaataagccg gataaggatc actcaataaa aaacccgggc tccaacccgg
     2581 gttttcagtt ttttctgcgc tgcgcgacac agccctacgc cgccttcttc gccggcctta
     2641 actgaccacg caaaaaattt ccttcctgct cgatcagcgt catcacccaa agggtgggcg
     2701 tcatcggcgg aacagcatct tcctcgccca gcgtgagcaa caccgggaca ccataatgag
     2761 gatgctgatc atcctccgac tcgaaactcc agtacaagcc gggcacgttc tttcgcaacg
     2821 tctcaccaac gtagcgggcg aaaagatcgc gcgcaaactt gttcttcctg tccttcattt
     2881 cttccgtagt gtaattctcg cggatatatt cttcgatcac atcgagagag gcaggggaat
     2941 aatccagtcg ggctcttact tttttgggga ggatgctgag gcggggcatt tcatcgggca
     3001 taatgttcag ccaccaggtg taattctctt ccttgcaatt gaggtcgctt acagttatca
     3061 tggggttatt gggtttatgc aagtataaaa aattcccgcc aatgaaaaaa gcatctttaa
     3121 aattcttatc aattgtcaaa tcactcgcta cccaatacga ccacattacc caatactaca
     3181 acgttacccg taaaacaacc gtttttcgct cagcgcttag tcataaacgt aactaccggc
     3241 tatcccatgg acatatattt taaatacata taatcaactt tttaaataat gcataaatca
     3301 ttaataaaca acaataaatg ccactatttg cacatttcct tggcaataca caataaaaaa
     3361 tcagctccgg cgtagcctcg cccatatcac tggggcatta tctttgcacc ggtaacaaaa
     3421 aaataattct ctattatgaa aaaactagca ttggtccttt ttgcaggtat ttccttcgca
     3481 actgcacacg cgcaggtgca gttcggggta aaagctggcg caaacttcgc cacgcagaca
     3541 gggagtgacg ccggtgattc caagacactg ttcaatttta atgcaggggc tttcttacgc
     3601 cttgccgtcg tccagggctt cagcatccag ccggaactgg tctattcggg tcagggcgca
     3661 tcctacgatg tgagcggcgg taccctgagg gaacacgcca attacctgaa catccccgtc
     3721 ctgttcaaat ggtcgcaccg tgcaggtccc tattttgaaa cgggtccgca attgggcttt
     3781 atcctcagcg ccaaggccag catcaacggt aacagcaccg attcgaaatc ggcctacaat
     3841 tccaccgact tcgcctgggt cttcggcgtt ggttacaaga tccccagaag cccggtgggc
     3901 atcgatctgc gttacaacat cggtatcgcc aacgtagaag accgcggtgt caccggtcag
     3961 accggctcct ggcgtaacga cgtagtgcag ctcggactca cctacgtgct gttcaacggc
     4021 ccaagaagat agtatcgttt gtttttagaa gtttgtatag aatgtttgtt acaagcggcc
     4081 gccctccggg cggccgcctt gttttcatga aaagtccaga attgtcaaaa atacccactt
     4141 agtggtattg tgcattcccg ccccccggat agctttgccc caaccaaaaa acctcactag
     4201 tatgaaaaaa cttg
//
LOCUS       DATGFG010000212         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1678074, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000212 DATGFG010000000
VERSION     DATGFG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678074"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VKQ52_03860"
     CDS             complement(<1..394)
                     /locus_tag="VKQ52_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86348.1"
                     /translation="MIKRLLFVVVLGLVVAGVVAQGVEPEVFAPGVVSGPVNDAAPAF
                     SPDGNTVYFHRSGPAMSGVILVSHRRNGVWSTPEIASFSGRWQDIEPAMAPDGSYLIF
                     ASNRPVAPGGKGLDGLWNSQHYPNGGGNL"
     gene            748..1590
                     /locus_tag="VKQ52_03865"
     CDS             748..1590
                     /locus_tag="VKQ52_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86349.1"
                     /translation="MNTLKNHVILYDSECPMCKLYTQAFTTSGMLDTDGRAPYQDMPA
                     FACPVVDWKRAVNEIALVDKTTGAVHYGIDSLFTIIANSCPIFKPLFSFKPFAWSMRK
                     LYAFISYNRKVIIPAPKPKDDGLAPGRSQTLQPSFHLGYRIAWLLFTWAIVGGILTAY
                     AGRLTALFPYGTSYREYLMCAIQILFQGTIVNLYAPAQRWDYLGNMMTISLAGALLLL
                     PMLALTAIIHLPPAIIFGWALFIAAGMLLEHIRRSKLLGFGSTLTITWVLYRLIILGL
                     LTFM"
     gene            1660..>2773
                     /locus_tag="VKQ52_03870"
     CDS             1660..>2773
                     /locus_tag="VKQ52_03870"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLZ86350.1"
                     /translation="MRQSRRDFIKTTGCLTIGFFLGGEAFVWPTPSQELPGDLRDNPR
                     INAWLEILADGRVRVLTGKIELGQGIRTAVAQMAAEELDLDMTQVEVLLAETGRTPDE
                     GYTAGSNSIEASAVAIRYAAAAARQRLLQLAAQKWAVPIDDLECSKGRITTQKHDRSI
                     SIADLLDGHQITDEVRMPVTLKPKDKYQFVGKAIPRDDTKRIVTGAPVYVHDLSFPGM
                     LHGRIIHPPSYGARLLEIDEQAIKKSATGLVKLVVNGSFLAVVTTGEYNAIKAQRLVQ
                     ANAKWADGPPLPELNTGQLAAYIKALPITSEKVKEKGDLSGLPAATTIKASYYKPYIM
                     HGSIGPSCAVALYDDNRLDIWTHSQGVYPLRETLAGL"
BASE COUNT          634 a         1054 c          584 g          501 t
ORIGIN      
        1 agagattccc accgccattc gggtaatgct gactgttcca aagaccatcg agccccttac
       61 ctcccggggc aacaggccgg ttggacgcaa agatcaggta agatccatcc ggggccatcg
      121 ccggctcgat atcctgccac cggccggaga atgacgcaat ctccggcgtt gaccaaacgc
      181 cgttccgccg gtgagaaaca aggatgacgc cggacattgc cggaccgctt ctatgaaaat
      241 aaaccgtatt cccatctggg ctaaaagccg gcgccgcatc attcaccggc ccagagacaa
      301 ccccgggcgc aaatacctcc ggctcgacac cctgcgcaac aaccccggca accacaagac
      361 ccaataccac gacaaacaac aaacgcttga tcataaactg tttttaagag caatatccgc
      421 ctccacccca acagcacaaa gcctattcaa cccatccccg gcccttttca atcaaactac
      481 cccactaccc cttcgtcccc agcccccgtt caccccccgt tcagccccga ctctccaccc
      541 actctccctg tgccaaccgt aactccatcc accccagccc cagcctctgc cacccaagcc
      601 cggcttccat cgtccatctc ccccagcccc cgttcacccc ccgactcccc acccgctccc
      661 ccttcgcccc ccaatcaaac ttttatttca ataacttttg aaaattcaga aactactatt
      721 aaatttgccc aagcaaactc ccccaccatg aacaccttaa aaaaccacgt catcctctac
      781 gactccgaat gcccgatgtg caaactctac acccaggcct tcaccacctc cggcatgctc
      841 gacaccgacg gccgcgcccc ctaccaggac atgcccgcct tcgcctgccc cgtagtagac
      901 tggaaaagag ctgttaacga gatcgccctc gtcgacaaaa ccaccggcgc ggtccactac
      961 ggcatcgaca gcctcttcac catcattgcc aactcctgcc ctatcttcaa acccctcttc
     1021 tccttcaaac ccttcgcctg gtcgatgcgc aaactatatg ccttcatctc ctataaccgc
     1081 aaggtcatca tccccgcgcc aaaaccaaaa gacgacggcc tcgccccggg tcggagccag
     1141 accctccagc cctccttcca cctgggctac cgcatcgcct ggctgctctt cacatgggcc
     1201 atcgttggcg gcatcctcac cgcctacgcg ggacggctga cagccctgtt tccatacggc
     1261 acatcctacc gggagtacct catgtgcgcc atccagatcc tcttccaggg cacgatcgtc
     1321 aacctctatg cccccgcaca acgctgggac tacctcggga atatgatgac catctccctc
     1381 gccggcgccc tcctcctcct gcccatgctc gccctgacgg ccatcattca cctcccaccc
     1441 gccatcatct tcggctgggc cctgttcatc gccgccggca tgctgctgga acatatccgc
     1501 cggtcaaaat tgctgggctt cggctccacc cttaccatca cctgggtcct ctaccggctg
     1561 atcatcctgg gattgcttac ctttatgtaa catttcgccc ctgccgtcta tgtaacattt
     1621 cacccctgcc gtcgacgtat ctcatcaata gtccttacta tgcgtcagag cagaagagac
     1681 ttcataaaaa ccaccggctg tctcaccatc gggttcttcc tcggcggcga agccttcgtc
     1741 tggcctaccc cctcccagga gctccccggc gatctccgcg ataacccccg catcaatgcc
     1801 tggctggaga tcctggccga cggccgcgtc cgcgtcctca ccggcaagat cgaactcggc
     1861 cagggcatcc gcaccgccgt agcccagatg gccgcggaag aattggatct cgacatgacc
     1921 caggtggaag tcctcctcgc ggaaaccggc cgcactcccg acgaaggcta cacagcgggc
     1981 agcaactcca tcgaagcgag cgctgtggcc atccgctacg ccgcggccgc ggcacgccag
     2041 cgcctgctcc aactggcagc acagaaatgg gccgtcccca tcgacgatct cgaatgcagc
     2101 aaaggcagga tcaccacgca aaaacacgac cgctccatca gcatcgccga tctcctcgac
     2161 ggtcaccaga tcaccgacga ggtccgcatg cccgtcaccc tcaagccaaa ggataaatac
     2221 caattcgtcg gcaaggccat cccccgcgac gacacaaaac gcatcgtcac cggggcgccg
     2281 gtatatgttc acgacctcag cttccccggc atgctccatg gccgtatcat ccatcccccc
     2341 tcctatggcg cgcgcctgct ggaaatagac gaacaggcta taaaaaaatc ggccaccggt
     2401 ctggtaaagc tggtcgtgaa cggcagcttc ctcgccgtcg tcacaacagg tgaatacaac
     2461 gcgataaagg cccaacgcct ggtacaggcc aatgccaaat gggccgacgg cccacccctg
     2521 ccggaactga acaccggtca actggccgca tatataaaag ccctgccgat cacatcggaa
     2581 aaggtcaaag aaaaaggtga cctctccggc ctgccggccg caacaaccat aaaagcaagc
     2641 tattacaaac cctatatcat gcacggctcc atcggccctt cctgcgccgt tgccctgtat
     2701 gatgacaacc gactggatat ctggacccat agccagggag tatacccgct gcgtgaaacc
     2761 ctcgccggcc tct
//
LOCUS       DATGFG010000213         5068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_421459, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000213 DATGFG010000000
VERSION     DATGFG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421459"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..929
                     /locus_tag="VKQ52_03875"
     CDS             171..929
                     /locus_tag="VKQ52_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86351.1"
                     /translation="MKFLKILLPIALAFCFTGCLDIDEKIDVKKDGSGQLSENMDMSQ
                     MIDILQTYMGKDEMAKKGLQNMDTTIYLKDVIDTSTSLSAEKKALLRPGSVHIKLNME
                     AKIFTTHMVFPFSSQENLQKLYTIMNDGSSLGNAKLFGNLGGDEAGGGSPDLSQFTGI
                     YNFVSRDGMMSKQVNKEKWKALTDNPQMAQMKQASQMGMEINYTTTILLPRPVKKVSN
                     PTAKMSDDKKSVTLKYNLIDALDHPEQFEYTIEY"
     gene            942..1700
                     /locus_tag="VKQ52_03880"
     CDS             942..1700
                     /locus_tag="VKQ52_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86352.1"
                     /translation="MTRPQSLCTALLALSLFACHQGQPAASGNAPGISQQLANSITTG
                     GTWTGHYSKGLLTLAINYISGNIASGYDIHKGLRRNLNGSVEQKGGRLEFVLREPGGN
                     PYDGTFYLSLDTATDKISGKWDPTDVTKAKAGPLELARSYIEDTLYIVRDQWYNDLGR
                     LSFGNEGVCILEYYPGTDTSGVNDPNAQLVTVKGSYEITGDTIRIDWQRNNRTPTVNM
                     KLIFHAGKEYTDSTNGVSPWLQGNGVKFDKFSAG"
     gene            1703..2467
                     /locus_tag="VKQ52_03885"
     CDS             1703..2467
                     /locus_tag="VKQ52_03885"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HLZ86353.1"
                     /translation="MRKLPLRIVLLVALAFFSFLMIRLTLPYTAMRSGVNFLRTKQYI
                     YHIRYWRISFYAHVFTSCLVLLAGFTQFAPRLLVRRPRIHRVMGWVYLIVVTTISGPA
                     AFVMALFANGGLPARTSFTLLSLLWISFTLFAGYYAFRRRFALHGAFMFRSYALTLSA
                     ITLRGYTYLIDLTTLPISPRDIYILTAWLAWVPNLIVAEVLIRRGWVEKIYRVPAPKP
                     EPPMLMPELPMPKPEPPNPELPIAGQGEHPPVGISH"
     gene            complement(2421..3548)
                     /locus_tag="VKQ52_03890"
     CDS             complement(2421..3548)
                     /locus_tag="VKQ52_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86354.1"
                     /translation="MKPLSALSLAATIFLFSCHNRPSAVTLTENSLYVIDTVRMAAAG
                     GDEKAARNKLGEAAGLYKKDTMAGIRLFKSSILLKPTAKAYFDLAGALLSTRQYDEGI
                     KALSVAEALGYTPLANVMFRYAYAYANKPDDTGHLPNSGYVIHYMELAIQMGYAHPGQ
                     FLQKDLFPNAMRKSDYDVVFTNTLSGGAVRDPGKPLWDAYRDQFPEIGLPLVIDRPWL
                     ENHKLDDPIDFQYEKFIPDMRDARFSREGGTSFYYVARIHKDQAFVALLYGAVDEVVE
                     GDESQNPRLALVTYDRQGKLIDQMTVAGYRVLSEPFLVCSIQPDLKFRVQGFKDVYQN
                     NPDSAGYDHNPVVRREPQSPQDFQIGANGKFQRADAPLALR"
     gene            3635..>5068
                     /gene="nadB"
                     /locus_tag="VKQ52_03895"
     CDS             3635..>5068
                     /gene="nadB"
                     /locus_tag="VKQ52_03895"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HLZ86355.1"
                     /translation="MEQTDFLVIGSGIAGLSYALKVAQHYPDKRVLVITKTRIDETNT
                     KYAQGGIAGVTDFEHDSYEKHIQDTLVAGDGLCNPRTVEIVVKEGPARIREIIDWGTR
                     FDRDDEGDFKLGREGGHSEFRILHHKDVTGKEIERAMLETVGKQKNIEIHKHWFVLDL
                     ITQHHLGYLVTKSTPDIECYGVYALDQHTNKIVKILSKITLLATGGNGQAYRTTTNPK
                     IATGDGVAMVYRAKGRIENMEFIQFHPTALYEPGVSPSFLITEAVRGDGGILRNDTGE
                     AFMERYDERRDLAPRDIVARAIDSEMKRTGTEHVFLDCRHMEIDKFVEHFPNIYAKCK
                     SIGIDVAEHMIPVAPAAHYSCGGIKVDEWGRTSIEHLYACGECSSTGLHGANRLASNS
                     LLEALVFAHRCYLDSVHRVEGIAFQEHIPDWNAEGTTEPDEMILITQSGKELQLVMTD
                     YVGIVRTHTRLQRAMKRLDLLHEEIESL"
BASE COUNT         1165 a         1381 c         1425 g         1097 t
ORIGIN      
        1 cacccgggac agtattgccg tggagaatct cagcggcgcg cccgactgtt ttaataataa
       61 gatgggtctt gtgttgctgc agcccgccca ttcacaaata tttaccgtac ggtacaaggt
      121 tagcgtcgac taaaaaacta aattgccctt caactcaaat aatgcattcc atgaagtttc
      181 tcaaaattct cctccctata gccctggctt tttgctttac cggttgtctc gatatcgatg
      241 aaaagatcga cgtaaagaag gatggcagcg gtcagctaag tgaaaacatg gatatgagcc
      301 agatgatcga tatcctgcaa acctatatgg ggaaggatga gatggcgaaa aagggtcttc
      361 agaacatgga tactaccatc tacctgaagg atgtcatcga tacgagcacc agtcttagtg
      421 cggagaaaaa agcgcttctc cggcccggga gtgtgcatat aaaactgaat atggaagcca
      481 agatctttac cacccatatg gtcttcccct tttccagcca ggagaacctt cagaaattat
      541 atactatcat gaatgatggg tcttcccttg ggaatgcaaa attgttcggc aacctcgggg
      601 gcgacgaagc cggcggcggc agcccggacc tcagccagtt tacgggtatc tacaatttcg
      661 tgagcaggga cgggatgatg tcgaagcagg tgaataagga aaaatggaag gccctgaccg
      721 ataatcccca gatggcgcag atgaagcagg cttcgcagat gggtatggag atcaattata
      781 ccacgaccat cctgctgccg cgaccggtga agaaggtgtc caaccccacg gcgaagatgt
      841 cggatgataa aaaatccgtc acgttgaagt ataaccttat cgatgcactc gatcaccccg
      901 agcagtttga atatacgata gaatattagt atatttctgg catgaccaga ccccaatcct
      961 tgtgtaccgc cctgcttgcc ctatccttat tcgcctgtca tcagggccag ccggctgctt
     1021 ccggcaatgc accggggatc tcgcaacaac ttgccaacag cattaccacc ggcggcacct
     1081 ggacggggca ctacagcaag ggactcctca cgctggcgat caattatatt tccggcaaca
     1141 tcgcttcggg ttatgatatc cacaaggggc tccgccggaa tctgaacggc agtgtggaac
     1201 aaaagggcgg ccggctggaa ttcgtgctca gggagccggg cggcaatccc tacgacggca
     1261 cattctatct ctccctcgac acggccaccg acaagatctc cgggaagtgg gatcccaccg
     1321 acgtcaccaa ggccaaggcg ggcccgctcg agctcgcgcg atcctatatt gaggataccc
     1381 tgtatattgt ccgcgaccaa tggtacaacg acctgggcag gctttccttc ggcaacgaag
     1441 gggtctgcat cctggaatat tatcccggta cggatacttc cggagtcaac gaccccaatg
     1501 cccagcttgt caccgtaaaa ggcagctacg agataaccgg cgataccatc cggatagact
     1561 ggcagcgcaa caatcgcacg ccgacggtca acatgaagct gatcttccat gcggggaagg
     1621 aatacaccga ttcgacgaat ggggtttctc cctggctgca gggcaatggg gtgaagtttg
     1681 ataaatttag cgcgggctga tcatgagaaa gttgccgtta cgaatagtgc tgttggtggc
     1741 cctggccttc ttttccttcc tcatgatccg gctcacccta ccgtatacgg ctatgcgcag
     1801 tggtgtgaac ttcctgcgaa ccaagcaata catctaccat atccgctatt ggcgcattag
     1861 tttttatgcg catgtcttca ccagctgcct ggtgctgctg gccgggttta cgcagttcgc
     1921 accccgtctg ctggtacgca ggccccggat ccatcgcgtc atgggctggg tatacctgat
     1981 cgtcgtgacg acgatcagcg gtccggcggc gttcgtcatg gccttatttg cgaacggcgg
     2041 actgccggcg cgaacgagct ttacactttt gtcgctgctt tggatatcct tcaccctctt
     2101 tgccgggtac tatgcttttc gccggcggtt tgccctccat ggggccttta tgttcagaag
     2161 ctatgcgctg accctctcgg ccattaccct ccggggttat acctatctta tcgatctgac
     2221 caccctgccg atcagtcccc gggacatcta tattctcacg gcatggcttg cctgggtgcc
     2281 caacctgatc gtggccgaag tgctcattcg ccgtggttgg gtggaaaaga tctaccgcgt
     2341 gccggcgccg aagccggagc cgccgatgct gatgccggag ctgccgatgc cgaagccgga
     2401 gccgccaaat ccggagctgc ctatcgcagg gcaaggggag catccgcccg ttggaatttc
     2461 ccattagccc ctatctggaa gtcctgcggc gactgtggct cccggcggac caccgggttg
     2521 tggtcatatc ctgcggagtc ggggttattc tgatatacat ccttgaagcc ctgtacccgg
     2581 aatttcagat cgggctggat gctgcatacc aggaagggct cggacagcac ccgatacccg
     2641 gccaccgtca tctggtcgat gagctttccc tgcctgtcat aggtgacgag ggccaggcgc
     2701 gggttctgcg actcgtctcc ctccaccact tcgtctaccg cgccatagag gagggcaaca
     2761 aaggcctgat ccttgtgtat ccgggccaca tagtagaaag aggtaccgcc ttcccgggag
     2821 aacctggcat cccgcatgtc ggggatgaac ttctcatatt gaaagtcgat ggggtcgtcc
     2881 aacttatggt tttccagcca gggcctgtcg atcaccaggg gcagcccgat ctcggggaat
     2941 tgatcccggt aggcgtccca aaggggttta cccgggtctc tcaccgcgcc gccgctgagc
     3001 gtgttggtaa acaccacatc gtaatcactc ttgcgcatcg cattcgggaa caggtctttc
     3061 tgcaggaact ggccaggatg cgcatagccc atctggatcg ccagctccat atagtggatg
     3121 acataaccac tgttgggcag atggcctgtg tcatccggct tgttcgcata ggcataggca
     3181 tagcgaaaca tcacgttcgc caggggcgta tatcccagcg cttcggcgac ggacaatgcc
     3241 tttatgcctt cgtcatattg gcgggtggaa agcaatgcgc cggcaagatc gaaataggcc
     3301 ttggcggtag gcttcagcag gatggaagat ttgaacagcc ggatgccggc catggtgtct
     3361 tttttgtata gtccggcggc ttctccgagc ttattgcggg ccgctttttc atcaccgcct
     3421 gctgccgcca tccggacggt atcgatgaca tatagggaat tttcggtaag cgtcaccgcg
     3481 gaaggacggt tatgacaaga gaagaggaag atcgtcgcgg caagtgataa ggcagataac
     3541 ggtttcatgg tgaagggtgt tacagcccaa gatagcaaat ttttgttacc ggcgaaagcg
     3601 taaacggtgc tacctttgca gctatttgaa gcttatggaa caaacagatt tcctggtcat
     3661 aggctccggt atcgccggct tgagctatgc cctgaaggta gcacaacact atccggacaa
     3721 gcgggtactg gtcattacca agacccggat cgacgagacg aataccaagt atgcccaggg
     3781 cgggatcgcc ggggttaccg atttcgaaca cgacagttac gagaagcata tccaggacac
     3841 ccttgttgcg ggtgacgggc tctgcaaccc gcgtacggtg gagatcgttg taaaggaagg
     3901 gccggcgagg atccgggaga tcatcgattg ggggacccgg ttcgacaggg atgacgaagg
     3961 cgatttcaaa ttgggcaggg aaggcggcca ctcggaattc cgtatcctcc accacaagga
     4021 tgtaacgggc aaggagatag agcgggccat gctcgagact gtcggcaaac agaagaatat
     4081 cgagatccac aaacactggt ttgtcctcga tctcatcacc cagcaccatc tcggctatct
     4141 tgtcaccaag tcgacgccgg atatcgaatg ttatggggta tatgcgctgg accagcatac
     4201 gaataagatc gtgaagatcc tttcgaagat cacgctgctg gctaccggcg gcaacgggca
     4261 ggcgtaccgg acgactacca acccgaagat cgccaccggc gacggcgtgg cgatggtgta
     4321 cagggcgaag ggacggatcg agaatatgga atttatccaa ttccatccta ccgcgctgta
     4381 tgagccggga gtcagcccct ccttcctgat caccgaggcc gtgcgcggcg acgggggtat
     4441 tctgcggaac gacacgggcg aagcctttat ggaacggtat gatgaacgca gggacctcgc
     4501 tccccgcgac atcgtggccc gggccatcga cagcgagatg aagcggacgg gtaccgagca
     4561 tgtctttctc gattgccggc atatggagat cgacaaattc gtggagcatt tccccaatat
     4621 ctatgcgaaa tgcaaatcca tcgggatcga tgtggccgaa cacatgatcc cggtggcgcc
     4681 ggcggcacac tacagttgcg gcggcatcaa ggtcgacgaa tggggccgta cttccatcga
     4741 gcacctgtat gcctgcggcg aatgctcttc cacggggctg catggcgcca atcgcctggc
     4801 gagcaattcc ctgctggaag cgcttgtctt tgcccatcgc tgttatctcg acagtgtcca
     4861 ccgcgtggag ggtatcgcat tccaggagca tatcccggac tggaatgcag aagggacgac
     4921 ggaaccggat gaaatgatcc ttatcacgca gagcggaaaa gagctacagc tggtgatgac
     4981 cgactatgtg ggtatcgtcc gtacgcatac ccgtctgcaa cgcgcgatga agcgcctcga
     5041 cctgctgcat gaagagatcg aatccctg
//
LOCUS       DATGFG010000214         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_190975, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000214 DATGFG010000000
VERSION     DATGFG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190975"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VKQ52_03900"
     CDS             <1..1167
                     /locus_tag="VKQ52_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ86356.1"
                     /translation="IDWLNKVINESILGAALIRVIHSQQLEYAKFLEANAKALGLGLR
                     ILKLFASLIPVIIFTANLATLTILALGGHYVIGGQMTLGNFSAFNNYLNMLIFPILVI
                     GFMSNVIAQAQASYGRISGVLNTPDPVETGTLAKELDGNIELKDVSVSFGEKHALKDI
                     SFTVAGGSKTAVIGPTAAGKTQLLYLLTGLNQPNSGIIEFDGHPLDRYKKEDFHRQVG
                     FVFQDSIIFNMSIRENIAFSDTVTDESLEKAIATAELRDFIDSLPEKLNTIVSERGTT
                     LSGGQKQRIMLARALAIEPRVLLLDDFTARVDTQTEQKILANVRQNYPGITLLSVTQK
                     IASIEDYEQIILLMEGEMIASGTHAVLMQTCPEYVQIYQSQRSTSHYEQPELQS"
     gene            1142..>2141
                     /locus_tag="VKQ52_03905"
     CDS             1142..>2141
                     /locus_tag="VKQ52_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ86357.1"
                     /translation="MSSRNYNLNSLTEEQPKSKTWPALRKLVGLLPEQRGKLILALIT
                     MVIYAILSMLPPVLIGFTINKVLLHKDALATMDVFGWHILSGTGYGLVLTVCSCLLVI
                     YLLNLGAVYLRTILMGGFGQHLLFTLRNAIFNKLQELPVAFFARNKAGDLISRINNDT
                     DKLNQFFSQSLMQFVSSIFIIAGAGIALVWLNPRLGLATLAPAVLMWGYTKLVSPWVK
                     KRNAISLKATGAMSAEIQESLGNFKVVLAFNRRDYFRQRFDTANKHNYSSALSAGIAN
                     NVFTPVYGLLSSMGQMVVLLYGVYLISTGHLEVGFLISYFMYTNSFYDPLRQIAALW"
BASE COUNT          481 a          678 c          539 g          443 t
ORIGIN      
        1 atcgactggc tcaacaaggt catcaatgaa agcatcctgg gcgcggccct tatccgggtc
       61 atccattccc agcagctgga atacgcgaaa tttcttgaag ccaacgcaaa ggcgttggga
      121 ctggggctgc ggatactgaa actgttcgct tccctgatcc ccgtgatcat tttcacggcc
      181 aacctcgcca ccctcactat tttagcattg ggcggccact atgtcattgg cggccagatg
      241 acgctgggga atttctccgc ctttaacaat tacctgaata tgctcatctt ccccatcctc
      301 gtgatcgggt tcatgagcaa cgtcatcgcg caggcccagg cttcctatgg ccggatcagc
      361 ggcgttctca acacccccga tccggtggag accggcaccc tggcaaaaga actcgacggg
      421 aacatcgaac tgaaggatgt gtccgtctcc ttcggggaaa aacacgccct gaaagacatc
      481 tccttcaccg ttgccggcgg ctcgaagacg gccgtcatcg gccctaccgc cgcggggaag
      541 acccaattgc tctacctgct taccgggctc aaccagccca acagcgggat catcgaattc
      601 gacgggcacc ccctcgacag gtataagaaa gaagacttcc accggcaggt gggctttgtc
      661 ttccaggaca gcatcatctt taacatgagc atccgggaga atatcgcctt tagcgacacc
      721 gtcaccgacg agtccctgga gaaggcgatc gccaccgcgg aactaagaga cttcatcgat
      781 tcccttcccg agaaactgaa cacgatcgtc tccgaacggg ggacgaccct ctccggcggc
      841 cagaaacagc ggatcatgct ggcacgcgcc ctggccatcg aaccccgggt actcctgctg
      901 gacgatttca ccgcccgcgt ggatacgcag acggaacaaa agatcctcgc caacgtccgg
      961 cagaactatc ccggcatcac cctgctttcc gttacccaaa agatcgcgtc catcgaagac
     1021 tacgagcaga tcatcctgct gatggaaggg gaaatgatcg cctccggcac ccatgccgtc
     1081 ttgatgcaga cctgcccgga atacgttcaa atctatcaat cacaaagaag caccagccac
     1141 tatgagcagc cggaattaca atcttaacag cttaaccgaa gaacaaccga agagcaagac
     1201 ctggcccgcc ctccgcaaac tggtgggact gctgcccgaa cagcggggta aactgatcct
     1261 ggcgctgatc accatggtga tctacgcgat cctcagcatg ctgccgcccg tcctcatcgg
     1321 ctttaccatc aacaaggtgt tattgcataa agacgcgctg gcgacaatgg atgtttttgg
     1381 ctggcatatt ctcagcggca ccggttatgg gctcgtcctc accgtctgca gctgcctgct
     1441 ggtcatctac ctgctgaacc tcggcgcggt ctacctgcgc accatcctca tgggtggttt
     1501 cggccaacat ttgctcttca ccctgcgcaa cgccatcttc aataaactgc aggagctgcc
     1561 ggtggccttc tttgcacgga acaaggccgg cgatctgatc tcgcgtatca acaacgatac
     1621 ggacaagctc aaccagttct tctcccagtc cctgatgcaa ttcgtgtcca gcatctttat
     1681 catcgcgggc gccggcattg ccctggtgtg gctcaacccc cggctcggcc tggctaccct
     1741 cgcccctgcc gtgctgatgt gggggtacac aaagctcgta tcgccctggg taaagaaaag
     1801 gaacgccatc agcctcaagg ccacaggcgc gatgagcgcg gagatccagg aaagcctggg
     1861 gaatttcaag gtggtccttg cctttaaccg ccgggactat ttccgccagc gtttcgacac
     1921 cgccaataag cacaactatt cctccgccct cagcgcgggc atcgccaaca acgtctttac
     1981 cccggtgtac ggcctgctgt ccagcatggg acaaatggtc gtcctactct atggggtcta
     2041 cctgatcagc acggggcatc tcgaagtagg ctttctcatc agctacttca tgtacacaaa
     2101 cagtttttat gatccgcttc ggcagattgc ggcgctctgg g
//
LOCUS       DATGFG010000215         4494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1219055, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000215 DATGFG010000000
VERSION     DATGFG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219055"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VKQ52_03910"
     CDS             complement(<1..782)
                     /locus_tag="VKQ52_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86358.1"
                     /translation="MLKNFLKVALRNLWKHKGYSSLNIFGLAMGMTCSLLILLWIRDE
                     RSIDNLHANGSRLYSIYERQYYDGKVSSGYYTPGVLPEQMKKDLPEVEASTGFTWMDK
                     STFQVGDKILKEQGRNASADYFRMFSYPLLAGTPATALATPVSLAISRKMANEFFGSP
                     QAAIGKSIRVENRKQFNITAVFEDLPDNVADKFDFLVNWTSWLEDNSWAKEWGNNGPQ
                     TLLLLRKDADPVAFEKKITHYLDAYNKEQSKSFRIELGMQRYG"
     gene            970..1539
                     /locus_tag="VKQ52_03915"
     CDS             970..1539
                     /locus_tag="VKQ52_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86359.1"
                     /translation="MRIFTVILCFTSLTLLLSSCSSSRKMSNYVPFTRDLRQKLEKEN
                     IDLKQVQFYVDQKLILNRNLGDQKVVVNSGVVKLENGKYINEVIIPTLTPGLCDAVDG
                     DRLMISFEKGNNALAFGPGSGYTFNEYVLYGTEWKNGTTLVTYDSNKYRARCGSCSDV
                     ASATLVIKKSVLDKMERKSRTLRGRTVEK"
     gene            complement(1514..2092)
                     /locus_tag="VKQ52_03920"
     CDS             complement(1514..2092)
                     /locus_tag="VKQ52_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86360.1"
                     /translation="MKACYTFFFGCLLWFLAPAIATAQNDFHPAAYTYNEGGTVISSN
                     VPLNEINTHAYRHFQRLFPTGATKEYWFVSADGYQVSFLLEGRHHQAFFDRHGGFRYS
                     LQYYPGKEIPRNPGDLIKAKYPGYEIDVVTEITDGEKTFHLVKIVSPLSIKTLSVADG
                     RIEVLEDLTNNRPTVAAAPVSASSLISLRSYP"
     gene            2244..3446
                     /locus_tag="VKQ52_03925"
     CDS             2244..3446
                     /locus_tag="VKQ52_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HLZ86361.1"
                     /translation="MSRRKRTPRLLKGYTYHNKVRLVHGGKDYFDTILQLIDAAQTTI
                     HLQTYIFDGDETGRLVSAALLRAAARKVEIYVLLDGYASQHLSREIIDEWKSAGIRFR
                     WFWPLLKAKKFYLGRRMHHKVIVVDAARGMAGGVNISDRYNDIGGVKAWLDRALLVEG
                     QAALKLHIVCRDMWTKAYWKTASPKKDGFTWVPAFVPEEECMVRVRRNDWVLGKNEIS
                     RSYVQMFRQAQSHIIVLSAYFLPGAILRKAMMQASRRGVAIRIIVGGVSDVPVSRLAE
                     QYMYRWLFRNKIRVFEYQDTVLHGKMATYDGIWMTDGSYNVNQISAYASVELNMDVRN
                     EPFATCVEQELETLIRDHCKEIIAEEYFQHTGPLRRLWQWGAYQTVRMLFFLFTFYWH
                     QDRETKRR"
     gene            complement(3443..4450)
                     /locus_tag="VKQ52_03930"
     CDS             complement(3443..4450)
                     /locus_tag="VKQ52_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLZ86362.1"
                     /translation="MDSLTHIVLGAAIGEVLGGRKLGRKALLIGAIANSLPDIDFLAS
                     FWLPAARDVWAHRGITHSFLFLLIMTPALSFFASRIWPGSGMTRRDWYRFFGWQLFTH
                     IFIDAFNAYGTGWFEPFSHQRIAFHVLFVADPFFSIWLGIAFIALLVLKANHPARRKW
                     AVAGLLLSSTYLCYGITNKLRIDRQVTADLQTQQHPYTRYFSTPTPLNNWLWYVVAED
                     SGGFYTGYRSLFDHEPTHFRFQPKAKWLLKPFLGQPDVHYLLRFSQGFYTVEEWGDTV
                     VFNDLRFGEMKGWEDPKARFVFHYFLQRPGENRVVVQRGRFAGWDRRTIRIFIRRIRG
                     N"
BASE COUNT         1012 a         1080 c         1406 g          996 t
ORIGIN      
        1 ccgtagcgct gcatgccgag ttcgatgcgg aaggatttgc tttgttcctt gttataggca
       61 tcgagatagt gggtgatctt tttttcgaag gcgacagggt cggcatcctt gcggagaagg
      121 aggagggtct gggggccatt attgccccat tccttcgccc agctgttgtc ctcgagccag
      181 gaggtccagt tgaccaggaa gtcgaatttg tcggcgacat tgtcggggag gtcttcgaac
      241 acggccgtga tgttgaactg tttccggttt tcgacgcgga tggacttgcc gatggcggcc
      301 tgggggctgc cgaagaactc gtttgccatt ttccgggaga tggccaggct taccggggtg
      361 gccagcgcgg ttgcgggtgt gcccgccagc aaggggtagc tgaacatgcg gaaatagtcg
      421 gcgctggcgt tgcggccctg ttccttgagg atcttgtcgc caacctggaa ggtggatttg
      481 tccatccagg tgaagccggt ggacgcttcg acctcgggga gatctttctt catctgttcg
      541 ggcaggacac ccggggtata gtagccggag gatacttttc cgtcgtaata ctggcgctcg
      601 tagatgctat agaggcggga gccgttggcg tggaggttat cgatactgcg ctcatccctg
      661 atccaaagga ggatgaggag gctgcaggtc atgcccatgg ccaggccgaa gatgttgagg
      721 gagctatagc ctttgtgttt ccagaggttg cggagggcga cttttaggaa gttctttagc
      781 atggcggcag tagtttgacc atatgagacg cagaagttgg gcggaatgtt gcaggaaggg
      841 atgggaaaca ggaaggggcg gaaaattggg acgccggatg cgccgatatt aacatttata
      901 taacacggca gggacgaaac tatttcgttt caaaaaggtc aaaccatgca taaataataa
      961 gaacgtatta tgagaatctt caccgtgatc ctttgtttta cttcgctgac cttattgtta
     1021 agcagctgca gcagcagccg gaagatgtcc aattatgtac cctttacccg ggatcttcgc
     1081 caaaagcttg aaaaagagaa cattgacctg aagcaggtgc aattctacgt ggatcagaag
     1141 ctgatcctca accggaatct gggcgaccag aaagtggtgg tcaattccgg ggtggtcaag
     1201 ctggagaacg ggaaatacat caatgaagtg attattccga cccttacccc gggtctttgc
     1261 gacgcggtgg acggcgacag gctcatgatc agcttcgaga agggtaataa tgccctggcc
     1321 tttggtccgg gcagcggtta tacctttaat gaatatgttc tgtacggaac ggaatggaag
     1381 aacggcacca cgctggtgac ctatgattcc aacaaatacc gggctcgttg cggcagctgt
     1441 tcggatgtcg cttcggctac ccttgtgatc aagaagagcg tgctggataa gatggagcgt
     1501 aaatccagga cccttagggg taggaccgta gagaaataag cgaggaggcc gaaacaggcg
     1561 cagccgccac agtcggccgg ttattggtca ggtcttcgag gacctcgatc ctgccgtccg
     1621 cgaccgagag ggtctttatg ctgagggggc tcacgatctt cacgaggtga aaggtctttt
     1681 ccccgtcggt gatctccgta accacatcga tctcgtaacc aggatactta gcctttatca
     1741 gatcccccgg atttcggggg atctcttttc ccgggtaata ctgtaaggaa tagcggaagc
     1801 ctccatgccg atcgaagaaa gcctggtgat gccggccttc cagcaggaag gagacctggt
     1861 agccatccgc cgacacaaac cagtattcct tcgtggcgcc ggtggggaag agccgctgga
     1921 aatgccggta ggcatgggta ttgatctcgt ttagcggaac attgctggaa atgaccgtcc
     1981 ccccttcgtt gtaggtgtag gcggccgggt gaaaatcgtt ctgggccgtg gcgatcgcgg
     2041 gggccaggaa ccaaaggagg cacccgaaaa agaaggtata gcaagctttc atcgtgtaga
     2101 gttttgttga atttgtctat ttaagttaac aatttaataa tacacgatga aatatttttt
     2161 tgtctatctt aattggtttt tttcgtccat tgaaatttgg tttcccgcaa aacccgatct
     2221 ttacccttcg accgctctca tctatgtcaa gacgaaaaag gaccccgcga ctgttgaagg
     2281 gttatacgta tcacaacaaa gtgcgcctgg tccacggcgg taaagactat ttcgacacga
     2341 tcctgcagct gatcgacgcc gcacagacaa ccatccattt acagacctat atttttgacg
     2401 gcgacgaaac gggccggttg gtctcggccg cgctgctgcg cgccgctgcc cggaaagtgg
     2461 agatctatgt cctgctggat gggtatgcct cgcaacatct ttcccgggag atcatcgacg
     2521 aatggaagtc ggcaggcatc cgtttccgtt ggttctggcc cctgctgaag gcgaagaagt
     2581 tctatctcgg aaggcggatg catcacaagg tgatcgttgt ggatgcggcg cggggaatgg
     2641 ccggaggggt gaatatcagc gaccggtaca acgatatcgg cggggttaag gcctggctgg
     2701 accgcgcgct gctggtggaa gggcaggccg ctttgaagct gcatatcgtc tgccgcgaca
     2761 tgtggacgaa ggcgtattgg aagacggcca gcccgaagaa ggacgggttc acctgggtac
     2821 cggcctttgt tcccgaagaa gaatgcatgg tcagggtgcg gcgcaacgac tgggtgctgg
     2881 ggaagaacga gatctcgcga agctatgtgc agatgttccg gcaggcgcaa tcccatatca
     2941 tcgtgttatc cgcctacttc ctgcccggcg ccattctccg gaaagcgatg atgcaggcct
     3001 cgcggcgggg ggtggcgatc aggatcatcg tcgggggcgt ctcggatgtg cctgtctcgc
     3061 gtctggcaga gcagtatatg tatcgctggc tgttccgcaa caagatcagg gtctttgaat
     3121 accaggatac ggtcctgcat gggaagatgg cgacctatga cgggatatgg atgaccgatg
     3181 gatcgtataa tgtcaaccag ataagcgcat atgcgagcgt cgagttgaat atggatgtcc
     3241 ggaacgagcc gtttgccacc tgcgtggagc aggagctgga gacgctgatc cgggatcact
     3301 gcaaggagat catcgcggag gagtattttc aacatacagg cccgttgcgg cggctctggc
     3361 agtggggggc ctatcagacc gttcgcatgt tatttttcct ttttactttt tactggcacc
     3421 aggatcggga gacaaaaagg cgctagttgc cgcggatccg ccggatgaag atgcgtatcg
     3481 tccgccggtc ccagccggcg aagcggcccc gttgcaccac taccctgttc tccccggggc
     3541 gttgcaggaa ataatgaaat acaaaccgcg ccttcgggtc ttcccatccc ttcatttcac
     3601 caaaccggag atcgttgaag acgacggtgt ccccccactc ttccaccgtg taaaagccct
     3661 ggctgaaacg gaggaggtag tgtacgtcgg gttggccgag gaagggtttt aagagccatt
     3721 ttgcttttgg ctgaaaccgg aaatgcgtgg gttcatggtc gaacagggag cggtagccgg
     3781 tgtaaaaacc gccgctgtct tcggccacca cataccagag ccagttgttg agcggggtcg
     3841 gcgtcgaaaa atagcgggta taggggtgtt gctgcgtctg caggtcggcc gttacctgcc
     3901 ggtcgatgcg cagcttattg gtgatgccat aacagagata cgtgctgctg aggagaaggc
     3961 cggcaacagc ccattttcgc cgcgcggggt gattggcttt taaaacgagc agggcgataa
     4021 aagcgatccc cagccagatg ctgaagaagg ggtcggctac gaagaggaca tggaaggcga
     4081 tgcgctgatg gctgaagggt tcgaaccagc cggtgccata ggcgttgaag gcgtcgataa
     4141 agatatgggt aaacagttgc caaccgaaaa agcgatacca gtccctgcgg gtcattccgc
     4201 tgcccggcca gatgcgggac gcgaagaaag agagcgcagg ggtcatgatc aggaggaaga
     4261 ggaaggagtg ggttatcccg cgatgcgccc acacatcccg cgcggcgggc agccagaaag
     4321 aagccaggaa gtcgatatcg ggcaggctgt tggcgatggc gccgatgagc agggccttcc
     4381 tgccgagttt gcggccgcca agcacttcgc cgatggcggc tccgagcacg atatgcgtta
     4441 gtgagtccat gcgctgccga agataggtct ccggcaaggg agatcaaaaa ttat
//
LOCUS       DATGFG010000216         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1102102, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000216 DATGFG010000000
VERSION     DATGFG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102102"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VKQ52_03935"
     CDS             complement(<1..923)
                     /locus_tag="VKQ52_03935"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HLZ86363.1"
                     /translation="MMKRRKFLQQSMLASAGVMVAGRGLGRDGESVAGVDFPVVRVPE
                     ARRKFKSQAVEKIIREVRTSAGNKEVGWLFENCFPNTLDTTVDHELIGGKPDTYVITG
                     DIDAMWLRDSSAQVWPYLPLCKEDKDLQQLIRGVIHRQAKCILKDPYANAFYKDETKI
                     SEWMATDLTDMKPGVHERKWEIDSLCYPIRLAHGYWQITADTSPFDADWMAAMEKIVQ
                     TFREQQRKEGKGPYHFMRKTEFATDSVPLRGYGYPTKKVGLIHSMFRPSDDATIYPFL
                     VPSNLFALSSLRNLRTLLDTMGTGKEMIETC"
     gene            complement(948..2285)
                     /locus_tag="VKQ52_03940"
     CDS             complement(948..2285)
                     /locus_tag="VKQ52_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HLZ86364.1"
                     /translation="MRQELKREISLLQATSINMIDMVGIGPFVMFSLVIRLVGSNLFI
                     WAWVFGAVTALIDGMIWSELGAAYPLAGGSYNFLKEAYGKKWGPLMSFLFVWQTCLQS
                     ALVVASAAIGFAQYFNYLIPLEWWEEKALSGLLVIVITALLYRNIRTIGKMAVFLWAG
                     VIIILLWIIISGFTHHQVAYSPLPQKGQTMFGLSFMLLIGHASVKTIYSYLGYYNVCH
                     LGGEIRDPGRNIPRSIFISIICIAILYLAMNFSVVGVIPWQEAMHSKFAISLFMEKIY
                     GPRTAQVATMLVLWIAFASLFAVVLGYSRVPYAAAADGNFFPWFARLHPRKNFPYVSL
                     LFVGGLGFICSLLLRLEDAINAILAMRILVQFVAQAIGVVLLRRRRGTEGLPFRMWLY
                     PLPVILSVSIWLFEWYSTGLAALLGLFLVACGTVVFFVARDIWFDAGAKKKIM"
     gene            complement(2282..3583)
                     /locus_tag="VKQ52_03945"
     CDS             complement(2282..3583)
                     /locus_tag="VKQ52_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLZ86365.1"
                     /translation="MTAFNRRGFLDALGTFSATAFLAALAKPAWSRNLDKALMNVEHR
                     APEQLAGDEDFWYYVQQSFTTSPELINLNNGGVSPSPKVVQEAMKRYLDFSNEAPSYY
                     MWRILDQGREPLRKDLARISGCSPEEVAIHRNASEALETVIFGLPLRAGDEVVVSKYD
                     YPNCINAWKQREQRDGVKLVWIDLDLPSEDNEYLASQYVKAFTAKTRVVHVTHVINWN
                     GQILPVRKIADAAHQRGIDVLVDGAHSFAHFEYTIPSLGADYFGTSLHKWLAACIGSG
                     MLYVRKEKIAGLYPLFAAPDAKSDNIRKFENLGTRPFFIEQAIGKAIDFYDMIGAARK
                     EQRLFYLKNYWMAAVKEIPNVKFYTSLKPGFGCAIGVVGVEGKSNVVGDLEGFLFTEY
                     KIHVTTINWEKIHGVRITPNVYTTTKDLDRLITAIREYAKA"
     gene            3620..>4372
                     /locus_tag="VKQ52_03950"
     CDS             3620..>4372
                     /locus_tag="VKQ52_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86366.1"
                     /translation="MKYYIIAGEASGDLHGSNLIKELKKQDAQADIRCWGGDLMQAAG
                     GVVVKHYRDLAFMGFVEVLTNLRTIFRNLDFCKQDILAYQPDALILVDYPGFNLRITK
                     WAHRQRRHTTIASTHLAPQTPAAPPASSAAPQASSASPQQTSSAAGPNSTPALHSPAP
                     ISAPQASPTSASGPATSPAGFRIIYYISPQVWAWKENRVKQIRECVDRMLVILPFEKE
                     FYRKWNYEVEYVGHPLVEEIDDYQAPTGAIPGF"
BASE COUNT         1016 a         1412 c         1065 g          879 t
ORIGIN      
        1 aagcatgtct cgatcatctc cttacccgtg cccatcgtgt ccagcagcgt acgaaggttc
       61 cgaagactgc tgagcgcgaa taaattcgaa ggcacgagga atggatagat cgtggcgtca
      121 tcgctgggcc ggaacatgga atggatcagc cccaccttct tcgtcgggta gccatacccc
      181 cgcagcggaa cgctatccgt cgcaaactcg gtcttccgca taaaatggta gggccccttc
      241 ccctccttcc gctgctgctc cctgaaggtc tgcactatct tctccatcgc cgccatccag
      301 tccgcatcga agggcgaagt atccgccgtg atctgccaat atccatgagc cagcctgatc
      361 gggtaacaca aactatcgat ctcccacttc cgctcatgta cgcccggctt catatccgtc
      421 agatcggtgg ccatccactc gctgatcttc gtctcgtctt tataaaaggc attggcatag
      481 gggtccttca ggatacactt cgcctggcgg tggatcacac cgcggatcag ctgctgcaga
      541 tctttatcct ccttgcaaag cgggagatag ggccacacct gggcgctgct gtcccggagc
      601 cacatggcgt cgatgtcccc ggtaatgaca taagtgtccg gcttgccgcc gatcagttcg
      661 tgatccaccg tcgtatccag cgtattggga aaacaattct cgaaaagcca gccgacttcc
      721 ttgttgccgg cactcgtccg cacttcccgg atgatctttt ctacggcctg gctcttgaat
      781 ttgcgcctcg cctccggcac ccgcaccacc gggaaatcca ctcccgcgac actttcgccg
      841 tcccggccca gtccgcgacc ggccaccatc acccccgccg aggccagcat actttgctga
      901 aggaattttc tgcgtttcat catatggact cacaatttcg gccgaatcta cattattttc
      961 tttttagcac ccgcatcaaa ccagatatcc cgcgccacaa agaacacgac ggtcccacaa
     1021 gccaccagga acaaccccag cagcgccgcc aaacccgtcg aataccattc gaacagccag
     1081 atcgacaccg acaggatcac cgggagggga taaagccaca tccggaacgg cagcccctcc
     1141 gtcccccttc gccgccggag caacaccacc ccgatggcct gcgcgacgaa ttgcaccagg
     1201 atgcgcatcg ccaggatcgc gttgatcgca tcctccagcc gcagtaagag gctacagata
     1261 aatcccagcc cgccgacaaa caggagcgac acataaggaa aattctttcg gggatgtaac
     1321 cgggcaaacc acgggaagaa attgccgtcc gcggcagccg catagggcac ccgcgaatac
     1381 ccgagcacca cggcgaacaa cgaggcaaag gcgatccaca acaccagcat cgtcgccacc
     1441 tgcgccgtcc gcgggccata gatcttctcc atgaaaagac tgatggcaaa cttcgaatgc
     1501 atcgcctcct gccaggggat cactccgacc acactgaaat tcatcgcgag ataaaggatg
     1561 gcgatacaga tgatcgagat aaagatactc cgggggatat tccgccccgg gtccctgatc
     1621 tcgcccccga gatgacaaac attataataa ccgagataac tatagatcgt cttgacggaa
     1681 gcatgaccga tcagcagcat gaacgacaac ccgaacatcg tctgcccctt ctgcggcaac
     1741 ggcgaatatg ccacctggtg atgcgtaaac ccactgatga tgatccacag caggatgatg
     1801 atcacgccgg cccacaagaa caccgccatc ttcccgatgg ttcggatatt ccggtacaaa
     1861 agggcggtga tgacgatcac cagcagaccc gagagcgcct tctcctccca ccattccaac
     1921 ggaataaggt agttgaaata ctgggcgaac ccgatcgccg ccgaagccac gacaagcgcg
     1981 gattgcagac aggtctgcca gacgaagaga aaggacatca gcggtcccca tttcttccca
     2041 taagcctcct tcaggaaatt ataactgccg cccgcgagag gataggcggc acccagttcg
     2101 ctccagatca tcccgtcgat cagcgccgtc accgcgccga atacccaggc ccagatgaag
     2161 agattactgc ccaccagccg gatcacgagg gagaacatca caaacggccc gatacccacc
     2221 atatcgatca tattaatgga ggtggcctgc aacaaactta tttcccgctt caattcctgc
     2281 ctcatgcctt ggcgtactcc cgtatcgcag tgatcagccg gtccagatct tttgtagtag
     2341 tgtaaacatt gggagtgatc cgcactccat gaattttttc ccaattgata gtcgtcacgt
     2401 ggatcttata ttcggtaaaa agaaaccctt ccaggtcgcc gacgacattg ctcttgccct
     2461 cgacaccgac gacgccgatg gcgcagccga aacccggttt caggctggtg taaaatttta
     2521 cattggggat ctccttcacg gccgccatcc agtaattctt caggtagaaa agccgttgct
     2581 cctttcttgc cgccccgatc atatcataga aatcgatcgc cttcccgatg gcctgctcga
     2641 taaagaaggg gcgcgtaccc aggttctcga acttccggat attgtccgac ttcgcgtcgg
     2701 gtgcggcgaa caagggatac aagccggcta tcttttcttt cctgacatag agcatcccgc
     2761 tgccgataca ggccgccagc catttgtgaa gactggtccc aaaataatcg gcgcccagcg
     2821 aaggaatggt atattcgaaa tgcgcaaagc tgtgtgcccc gtcgacaagc acatcgatcc
     2881 cgcgctgatg ggcggcatcc gcgatcttcc ggacaggcag gatctggccg ttccagttga
     2941 tcacatgcgt gacatgcacc acccgggtct tcgcggtaaa ggcctttaca tactgggaag
     3001 cgagatattc attgtcttcg ctcgggaggt cgaggtcgat ccataccagc ttgacgccgt
     3061 cgcgctgctc ccgttgcttc cacgcattga tacagttggg ataatcgtat ttggagacca
     3121 ccacctcatc tcccgctctg agcggcaggc caaagatcac cgtttcgagc gcttccgagg
     3181 cattgcggtg gatggccacc tcttcagggg aacaacccga tatccgcgcc aggtcctttc
     3241 gcaggggttc gcgtccctgg tcgagtatcc gccacatata atagctgggg gcttcattcg
     3301 aaaaatcgag gtagcgcttc atggcttcct gcaccacctt gggcgatggc gataccccgc
     3361 cgttgttgag gttgatcagt tcggggctgg tggtaaaaga ctgctgtaca taataccaga
     3421 aatcttcgtc accggccagt tgttcgggtg cccggtgctc cacattcatc agcgccttgt
     3481 ccagatttcg gctccatgcg ggcttggcaa gggccgccaa aaaggcggtg gcggaaaagg
     3541 tacccagcgc atccaggaag ccgcgacgat taaaagcagt catgaggtgt aagttaagaa
     3601 aaatctatct ttgccccgca tgaagtatta tatcattgcc ggcgaagcct ccggcgacct
     3661 ccatggcagc aatctgataa aagaactaaa aaaacaggac gcacaagccg atatccgctg
     3721 ctgggggggt gatctgatgc aggccgcggg tggggtggtg gtcaaacact acagggatct
     3781 cgccttcatg ggcttcgttg aagtactcac caatctccgc accatcttcc gcaacctcga
     3841 cttctgcaaa caggatatcc tcgcctatca acccgacgcc ctcatcctcg tcgactaccc
     3901 cggcttcaac ctccgtatca ccaaatgggc ccaccgccaa cgccggcaca ccactatcgc
     3961 ctccacccac ctcgcgcccc aaacccccgc tgcccctccc gcatcctccg cagccccgca
     4021 agcctcatcc gcctctcccc agcaaacctc atccgccgcc ggtcccaact ctacccccgc
     4081 cctgcactcg cccgcaccca tctcggcccc gcaagcctca cccacttccg cctctggccc
     4141 cgcaacctca cccgccggct tccggatcat ctattatatc tctccccagg tctgggcctg
     4201 gaaagaaaac cgcgtaaagc agatccgcga atgcgtggac cgcatgctcg tcattctgcc
     4261 cttcgaaaag gaattctacc ggaaatggaa ctatgaggta gaatatgtcg gccatccgct
     4321 ggtggaagag atcgacgatt atcaggcccc cacgggggcc attcccggct tc
//
LOCUS       DATGFG010000217         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1014069, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000217 DATGFG010000000
VERSION     DATGFG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014069"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VKQ52_03955"
     CDS             <1..191
                     /locus_tag="VKQ52_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HLZ86367.1"
                     /translation="KRLQKEFPAIPLLPVDERNTSKMASRAMIDMGMKKKQRRNKALV
                     DEIAATIMLQEYLERVSG"
     gene            235..813
                     /gene="def"
                     /locus_tag="VKQ52_03960"
     CDS             235..813
                     /gene="def"
                     /locus_tag="VKQ52_03960"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLZ86368.1"
                     /translation="MILPIVAYGSPILRVVGKDITPDYPGLSKLIEDMWETMYYSSGV
                     GLAAPQINRDIRLFVVDSMQIFNNLEDDEQDKYPDHPGIKQVFINAHIVKLNGEEWAY
                     TEGCLSIPKIREDVLRNEEVTLEYMDEQFQPHTTTFNGITARVILHEYDHIEGKLFID
                     YLKPLKRKLLKGKLDDISRGRISVDYKMSFPK"
     gene            complement(838..1974)
                     /locus_tag="VKQ52_03965"
     CDS             complement(838..1974)
                     /locus_tag="VKQ52_03965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLZ86369.1"
                     /translation="MNKAPDQRKVSKEGKAPETNEKVTTVQDEDNELDEGASPDGSDE
                     LYERMNLKVDKGQEPMRIDKFLVQRIESASRNKIQKALEAGMVLVNNKQVQPNYKIRP
                     QDEIVMYSDKEVHGEEIVPEEMPLNIQYEDEEVMIINKPPGLVVHPASGNYTGTLING
                     VAWYLQQKNHAVNEKNLPRFGLVHRIDKNTSGLMVLAKTEKAVRSLAKQFFDHTVKRQ
                     YTALVWGDLVEDKGTIVAHVGRHRRFRKLFDAYPDGDYGKDAITHYEVLERFGYTTLV
                     QCVLETGRTHQIRVHMQHIGHPLFNDDTYGGDKIVKGTVFSKYRQFVDNCFAICPRHA
                     LHAKTIGFVHPGTGEEVLFNSELPEDMAKLIEKWRGYTANKPLQ"
     gene            2095..>2509
                     /locus_tag="VKQ52_03970"
     CDS             2095..>2509
                     /locus_tag="VKQ52_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86370.1"
                     /translation="MEKVIFRDLGKMDYQQAWDYQETLLQENLLIKSESRRHMEERAI
                     EAGVLAGHLTTDQKVSEVNPADIPSTRHYLLFVEHPPVYTLGKSGHQENVLIGEDKMQ
                     EKGIQFFRTNRGGDITFHGPGQIVGYPVLDLEKFYT"
BASE COUNT          589 a          605 c          667 g          648 t
ORIGIN      
        1 tgaagcggct gcagaaggag ttcccggcga tcccgttatt gccggtggac gagcgaaata
       61 cttccaaaat ggcgtcccgc gctatgatcg atatggggat gaagaagaaa cagcggagga
      121 acaaggcatt ggtggatgag attgcggcca cgatcatgct ccaggagtat ctggaacggg
      181 tgtcgggttg aaaccgatat tttgcgtaat tttgcaccaa ttttcgctga aattatgatt
      241 ttacccattg tagcatatgg ttcccccatt ttgagggtag taggtaagga tataactccc
      301 gattatcccg gtctttccaa gctgatcgag gatatgtggg agacgatgta ttatagcagc
      361 ggggtgggcc tggccgcgcc ccagatcaac agggatatcc ggttatttgt ggtagactcc
      421 atgcagatct tcaataacct ggaagatgac gagcaggaca aataccccga ccatccgggt
      481 atcaagcagg tatttatcaa tgcccatata gtgaagctga atggggagga atgggcttat
      541 accgaagggt gcctcagcat tcccaagatc agggaagatg ttctccgcaa tgaggaggta
      601 accctggaat acatggacga gcaattccag ccccatacga cgaccttcaa tgggatcacg
      661 gcgagggtga tcctgcacga atacgatcat atcgaaggca aactgttcat cgattacctg
      721 aaacccttga aacgtaagct gctgaaaggc aagctggacg atatctcaag gggccggatc
      781 agcgtcgatt ataagatgag cttcccgaaa taatctactg taacggtctg aaataaccta
      841 ttgtaacggt ttattagctg tatatccccg ccatttttct atcagcttag ccatgtcttc
      901 gggcagctcg ctgttgaaca ggacttcctc gcccgttccg ggatgtacga atccaatggt
      961 tttggcatgc aaggcgtgcc gggggcagat cgcgaagcaa ttatccacga attgacgata
     1021 cttgctgaat acggtgcctt tcacgatctt gtcgcctccg tatgtatcgt cattgaacag
     1081 cgggtgcccg atatgctgca tatgaacgcg gatctgatgg gtacgtcctg tttccagcac
     1141 gcattgaacc agtgtggtat atccgaatcg ttccagtacc tcatagtgag tgatcgcatc
     1201 cttgccataa tctccgtcag gataggcgtc gaataatttc cgaaaacgcc ggtgcctgcc
     1261 tacatgagcg acgatcgtcc ctttgtcctc aaccaggtcg ccccatacga gggccgtata
     1321 ctgccgcttc acggtatggt cgaagaattg tttggccagg ctgcggacgg ctttctccgt
     1381 cttggccagc accatcagcc cgctggtatt tttatcgatc cggtgaacga gaccgaaccg
     1441 gggcaggttc ttttcattga cggcatggtt tttctgctga agataccagg cgaccccatt
     1501 gatcagggtg ccggtgtaat tgccactggc gggatgtacg acgagtcccg gtggtttatt
     1561 gatgatcatc acttcctcat cctcatattg aatattcagc ggcatttctt cggggacgat
     1621 ctcctcccca tgtacctcct tatcggagta catgacgatc tcgtcctggg gcctgatctt
     1681 ataattgggt tggacctgct tattattgac caataccatc cccgcctcca atgccttctg
     1741 gatcttgttc cgggaagcgc tttcaatccg ctggaccagg aacttgtcga tgcgcatggg
     1801 ctcctgtccc ttgtctactt tcaggttcat gcgctcataa agctcatccg acccgtcggg
     1861 tgacgcgcct tcatccagct cattgtcttc atcctgaacc gtggtaactt tctcgtttgt
     1921 ttctggcgcc ttgccttctt tcgatacctt tcgctgatcc ggtgccttgt tcatacctgg
     1981 gatttctgca aaaataggat ttagttggta aggcggctct gctgactttt atggtttgcg
     2041 ggtcgaggtg tgtagtaagt cagaagatca agaaataggg taaatttgca gcttatggaa
     2101 aaggtcatat tcagggattt gggcaagatg gattatcaac aggcctggga ttaccaggaa
     2161 acgctgttgc aggaaaacct gttgatcaaa tccgagtccc ggcggcatat ggaggagcgg
     2221 gctatcgaag cgggcgtatt ggcgggccat ttgactacgg atcaaaaggt ctcggaggtg
     2281 aacccggcag atataccgtc cacccggcat tatctccttt tcgtggagca ccctcctgta
     2341 tatacgctgg gcaagagcgg ccatcaggaa aatgtcctga tcggggagga taaaatgcag
     2401 gagaagggga tacagttttt caggaccaac aggggcgggg atattacatt ccacggtccc
     2461 gggcagatag taggctatcc tgtcctcgac ctggaaaaat tttatacgg
//
LOCUS       DATGFG010000218         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_420999, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000218 DATGFG010000000
VERSION     DATGFG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420999"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VKQ52_03975"
     CDS             <1..199
                     /locus_tag="VKQ52_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86371.1"
                     /translation="SFATQALLEEAQADEIKETTATAPSGKSKLRGIFRRVSRTFGKT
                     ADRDSDGQKEVLISAFQVALK"
     gene            228..1082
                     /locus_tag="VKQ52_03980"
     CDS             228..1082
                     /locus_tag="VKQ52_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ86372.1"
                     /translation="MNRVFLLTAGLCIALFCRAQNDTTGIGKVTAPAGDTLRVGNLLI
                     IRNGAKDFSGDAPSLRIKSRRERHYYRPSIISTNWGIVDLGLAGINDKTIYTSAAAQQ
                     FAPGANANWFKMRYGLLPRTVDVNIWVFMQRISLINHIVNLKYGLGIELNNYMYEYNL
                     RYLTNPTKIIMDTIQYSKDKLATDYVTLPVMLNFNFTPHRRHPFGVSAGASFGYLYSS
                     RQKLISHQHGKEKIYNDFDLNAWKISYIGELQLGWINLYGSYATRSIFGKGLDMVPYN
                     FGIRIGNW"
     gene            1236..1793
                     /locus_tag="VKQ52_03985"
     CDS             1236..1793
                     /locus_tag="VKQ52_03985"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLZ86373.1"
                     /translation="MRSKKLLLGSLLLVSLVSFRDAHHRSSSRRFFHRVPDGDVYLVV
                     IKSNYELQVYDKDGWYATYPAVFGSKSLDDKMMQGDRKTPEGIYHIVSKRPHEKWSRI
                     MDLDYPTAADVAKFKDRKARGLVPKTATIGDGIAIHGTWPHDDNAVDAYQMWTNGCVS
                     LKNEDMDEVYRITPVGTKVIIQREP"
     gene            complement(1790..>2269)
                     /gene="prpE"
                     /locus_tag="VKQ52_03990"
                     /gene_synonym="yahU"
     CDS             complement(1790..>2269)
                     /gene="prpE"
                     /locus_tag="VKQ52_03990"
                     /gene_synonym="yahU"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="catalyzes the formation of propionyl-CoA using
                     propionate as a substrate; PrpE from Ralstonia
                     solanacearum can produce acetyl-, propionyl-, butyryl- and
                     acrylyl-coenzyme A, and Salmonella enterica produces
                     propionyl- and butyryl-coenzyme A; not expressed in
                     Escherichia coli when grown on propionate/minimal media;
                     ATP-dependent; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HLZ86374.1"
                     /translation="YFATYPKLYFTGDGALRDADGNYRITGRVDDVLNVSGHRIGTAE
                     VENAINMHTGVVESAVVGYPHDIKGQGIYAYVIFNGTKTDDESIRKDIVQTVARIIGP
                     IAKPDKIQLVSGLPKTRSGKIMRRILRKIAEGELQNLGDTTTLLDPAVVDEIKKGRV"
BASE COUNT          539 a          716 c          554 g          460 t
ORIGIN      
        1 ctctttcgcc acccaggcct tgctggaaga agcccaggcg gatgaaataa aagaaacgac
       61 agccacggcg ccgtccggca agagcaaact acggggtatc ttcagacggg tatcccgcac
      121 ctttggaaaa acggccgatc gcgacagcga cggtcaaaaa gaggtgttga tcagcgcctt
      181 ccaggttgca ctcaaatagc attctcagca tttaaaaaaa acagaacatg aaccgggtat
      241 tcttactgac ggcaggattg tgcattgccc tcttctgccg ggcacaaaac gacacgacag
      301 gcatcggaaa ggtaacggcg cccgcagggg acacgctccg cgtcggcaat ctgctgatca
      361 tccgcaacgg ggcgaaagac ttcagcggcg acgccccctc gctccgcatc aaatcccgcc
      421 gtgaacggca ttattaccgc ccatccatta tcagcaccaa ctggggtatc gtcgatctcg
      481 gcctcgccgg cataaacgac aagacgatct atacctccgc ggccgcccag caattcgcac
      541 ccggcgccaa cgccaactgg ttcaagatgc gctacggcct gctgcccagg accgtcgatg
      601 tcaatatctg ggtctttatg caacgcatca gcctgatcaa ccatatcgtc aacctgaagt
      661 atggtctcgg catcgagctc aataactaca tgtacgagta caatctccgc tacctcacca
      721 acccgacgaa gatcatcatg gacaccatcc agtatagcaa ggataaatta gcgacggact
      781 atgtcaccct gcccgtgatg ctcaatttca actttacccc gcatcgccgg catccgttcg
      841 gcgtcagcgc aggcgcgagc ttcggctatc tctattcctc ccggcagaag ctgatcagcc
      901 atcaacacgg gaaggagaag atctacaacg atttcgatct caatgcctgg aagatctcct
      961 acatcgggga gctccagctg ggctggatca atctctacgg cagctatgcc acccgcagca
     1021 tctttggaaa gggcctcgac atggttccct acaacttcgg catccgtatc ggtaactggt
     1081 agtcccgctc cgcggcgact gccccagccg gccgttgcta acatccacca cccgctccac
     1141 ggagccggcc gccacgacac caaattgtta aaactttcct tacccgaaat aatccggcaa
     1201 cgcgcgacgt tggaactacg ttaaacagcc gtattatgcg ttccaaaaaa ttgctccttg
     1261 gcagcctact cctggtgagt ctcgtctctt tccgcgacgc ccaccaccgc agctccagcc
     1321 ggcggttctt ccaccgggtc cccgatggcg acgtctatct cgtggtgatc aagtccaact
     1381 atgagttgca ggtgtatgac aaggatggct ggtatgccac ctatcccgcc gtgttcggga
     1441 gtaaatcact ggatgataaa atgatgcagg gcgaccgtaa aaccccggaa gggatctatc
     1501 atatcgtttc caaacggccg cacgaaaaat ggtccaggat catggacctc gattatccca
     1561 ccgcggcgga cgtcgccaaa ttcaaagacc ggaaggcccg gggcctcgtc cccaaaaccg
     1621 ccaccatcgg cgacggcatc gccatccatg gaacctggcc gcatgacgat aatgcggtcg
     1681 acgcctacca gatgtggacc aatggctgcg tctcactaaa gaacgaggac atggatgaag
     1741 tctaccggat cacccccgtg ggcaccaagg tcatcatcca gcgcgaacct taaacccggc
     1801 ctttttttat ctcatccacc accgccggat ccagcagggt agtcgtatca ccaagattct
     1861 gcagctcgcc ctccgctatc ttccgcagga tccgccgcat gatcttcccg cttcttgtct
     1921 tgggcagccc gcttaccagc tggatcttgt ccggtttggc gataggcccg atgatccggg
     1981 ccacggtctg gacaatatcc ttccggatgg attcatcatc cgtctttgtc ccattgaaaa
     2041 tgacataggc atagatgccc tgtcccttta tatcgtgggg ataaccgaca accgcactct
     2101 ccaccacacc cgtatgcata ttgatggcat tctccacttc cgcggtgccg atacggtgac
     2161 cgctgacatt gagcacgtca tccacacggc cggtgatccg gtagttccca tcggcgtccc
     2221 gaagggcgcc gtcaccggta aaatacagct tggggtaggt agcgaaata
//
LOCUS       DATGFG010000219         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_204207, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000219 DATGFG010000000
VERSION     DATGFG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204207"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VKQ52_03995"
     CDS             <1..617
                     /locus_tag="VKQ52_03995"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HLZ86375.1"
                     /translation="SQVGWDQVEMKLPPGGKWRKMDCYQFNSVPDPMRIMHIKFRYWG
                     IFPFEARDKYQDGHGNLLIRLCRLVTIGDLRGKEVDASELVTLLSEALLLPAYAVQPY
                     IRWSAIAADRVGAELSWNGTRVHGLFFFNSRGEFERFETTDRWKAAGRGRFEPMAWSV
                     RVSDYVTRNGVRQPSRAAAAWLENGQWTDYFKGEITGTSGLAPA"
     gene            828..1427
                     /locus_tag="VKQ52_04000"
     CDS             828..1427
                     /locus_tag="VKQ52_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ86376.1"
                     /translation="MKRTLIPGNRRFWPLLFFFLCIGLFSVAQSRYAGSTVDLIVSGT
                     STLHDWNMKSVKADCSGVFSLNGAGQITAVEGLVFSTPATALKSEHTSMDNNAYKALK
                     TDRNPVIVYTVTSVSVAAANGGATLVTCNGKLSIAGATRDEQIVALCKPNGDNTISVS
                     GTEKISMKDFSIEAPTFMLGTIKTGNDITLSFALQLKRS"
     gene            1457..1864
                     /locus_tag="VKQ52_04005"
     CDS             1457..1864
                     /locus_tag="VKQ52_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86377.1"
                     /translation="MKKIITSGLIAGVVLLVLSILGLYATIWFFPTLAAQYYDPAFDS
                     QSGRYMIYYAHPFVIGLALAWFWDHFKVVLKGSFLTRGIEFGLAYALVAIFPVMWLTY
                     SALSVSLAMVGTWLAFGLLQGIIAGLVFEKTNP"
     gene            1883..2491
                     /locus_tag="VKQ52_04010"
     CDS             1883..2491
                     /locus_tag="VKQ52_04010"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ86378.1"
                     /translation="MKPYLFFLIPVFLLAGAGEPERDLYVLSRGYTVTIAGTSNLRDW
                     KESVSQVSGAMEAEVNADGSIALHSIRISMEVLSIKSDMGRLMDNKTYEALKAADHPE
                     ILFTLSTPMNLTQVRDCERAIPVKGSLLLAGVCKPVTMLVKTIEVSQGKLQFEGCENL
                     RMTDFGVRPPSALFGTMRASPDITIHFKTNFINQSISNNLKN"
     gene            2494..>3315
                     /locus_tag="VKQ52_04015"
     CDS             2494..>3315
                     /locus_tag="VKQ52_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86379.1"
                     /translation="MKKLLTIILLATGLTAAAQQGAMQFYRPSDKTGLNVFETTKNDT
                     TSFHQLHVKVGGNFEETFQNLYNKNTASAMTLPGYTGNVNSLESLTPGFNLANANLNL
                     DVQVADGIRVNLTTYLASRHHENVWVKGGYIQFDKLLFLHSAFVDNIMKSVTIKVGQF
                     DVNYGDQVFRRTDGGNTIYNPFIENYILDEFATEIGGEVIYHHKSGLFALGGITNGEL
                     DPTVLGSSKIDSATGKVNGYDAAWHGKIGYDKQLTPDLRVRLSGSFYTVKSANSNT"
BASE COUNT          775 a          885 c          917 g          738 t
ORIGIN      
        1 attcacaggt ggggtgggat caggtggaga tgaagctgcc tcccggcggc aagtggcgga
       61 agatggactg ttaccagttc aattcggtgc ccgatccgat gcggatcatg catattaagt
      121 ttcggtactg ggggatattt ccttttgagg caagggacaa ataccaggat gggcatggga
      181 atctgcttat ccggctttgc cgcctggtga cgatcgggga tctgcgggga aaggaggtgg
      241 atgcttcgga gctggtcacg cttttgtccg aggccttgtt gttacccgct tatgcggtgc
      301 aaccgtatat ccggtggtcg gcgatcgccg cagaccgtgt aggggccgag ctctcctgga
      361 acggtacacg ggtgcatggg ttattctttt tcaacagccg gggagaattc gagcgtttcg
      421 aaacgaccga ccggtggaag gcggcgggcc gggggcgttt tgagccgatg gcctggagcg
      481 tccgggtcag cgactatgtc acccggaatg gggtgcggca accctcccgg gcggctgcgg
      541 cctggctgga gaacgggcaa tggacggatt atttcaaggg ggagatcacg ggtaccagcg
      601 ggctggcgcc ggcgtgatgc cggctgaaca gcggcatcag ggataccatc ttataacggg
      661 gaagcacagg agtgcttcct ttttttattt gtaccgcgcc tgatctttct catgagtgtc
      721 cgggattgaa tcatccgaat tgatgatcaa cgtcatctcc ggccaatcgc ccgcgggata
      781 cttttacacc gtcaatcaaa aaagacgaaa accaaaaaac agtcattatg aaacgcacac
      841 ttatcccggg aaacagaagg ttctggccgc ttcttttctt cttcctttgc atcggccttt
      901 tttcggttgc ccaatcacgc tatgccggca gcacggtaga cctgatcgtc agcggcacct
      961 cgaccctcca tgactggaat atgaaatcgg tgaaggcgga ttgctccggt gtcttttcgc
     1021 tcaatggcgc cgggcagatc acggcggtag aagggctcgt tttcagcacc ccggccaccg
     1081 ccctgaagag cgaacatacc agcatggaca acaatgccta taaggcgctg aagaccgaca
     1141 ggaacccggt gatcgtctat acggtgacct cggtgagcgt cgctgcggcc aatggcgggg
     1201 ccaccctggt gacctgtaat ggcaaattat ccattgcggg cgccacgcgt gacgaacaga
     1261 tcgtcgccct ttgcaaaccc aatggggaca acacgatcag tgtatcaggc acagagaaga
     1321 tcagtatgaa agactttagc atcgaagccc cgaccttcat gctgggtacg atcaaaacag
     1381 gcaatgatat cacgctttct ttcgccttac agctaaaacg gtcctagccg attgtacacg
     1441 tacaaatcaa ccactcatga agaagataat cacctcgggc ctgatcgcag gcgtggtttt
     1501 actggtcctg agcatcctcg gactatatgc cacgatctgg ttcttcccca ctctggcggc
     1561 gcaatattac gatcccgcct tcgatagcca atccgggagg tatatgatct attacgccca
     1621 tccctttgtg atcgggctcg ctctcgcctg gttctgggat cactttaagg tagtcctgaa
     1681 gggctccttc ctgacgcggg gtatcgaatt cggcctggcg tacgccctgg tcgccatctt
     1741 cccggtgatg tggcttacct atagtgcgct gagtgtatca ctcgcgatgg ttggtacctg
     1801 gctggctttc gggctgctgc aaggtattat cgcgggtctt gtattcgaaa aaaccaatcc
     1861 ttaaccagat atctcatcag caatgaaacc gtatttattc ttcctgattc ctgttttcct
     1921 gctggccggc gccggtgaac ccgagcggga tctgtacgtg ctgtccaggg gatatacggt
     1981 gacgatcgcc ggcacttcaa acctgcggga ctggaaagag agtgtcagcc aggtgtccgg
     2041 cgccatggaa gccgaagtaa atgctgacgg aagcatcgcg ctccactcga tacgcatcag
     2101 catggaagtc ttatccatca aaagtgatat gggcaggctg atggacaaca agacgtacga
     2161 agcgctgaag gctgccgacc atcccgagat cctgttcacg ctgagtaccc cgatgaacct
     2221 gacccaggtg agggattgcg aaagggccat ccccgtaaag ggcagcctgt tgctggcagg
     2281 ggtgtgtaaa ccggtgacca tgctggtaaa gaccatcgag gtcagccagg gaaaattgca
     2341 gttcgaagga tgcgagaatt tacgaatgac cgattttggg gtcagaccgc cgtcggctct
     2401 ttttggaacg atgcgggcct ctcccgacat cacgatacat tttaaaacaa atttcatcaa
     2461 ccaatctatc tctaacaatt taaaaaattg acaatgaaaa agttattaac gatcatttta
     2521 ctggccaccg gcctgactgc cgctgcgcag caaggagcga tgcaattcta tcgccccagc
     2581 gacaaaacgg gtttgaatgt cttcgagacc accaagaatg ataccacctc cttccatcag
     2641 ctgcacgtca aggtaggcgg caatttcgaa gagacctttc agaacctgta taacaagaat
     2701 acggcctcgg ctatgaccct acccggctac accggcaacg taaacagcct ggaatcgctg
     2761 acccccgggt tcaacctggc caatgccaac ctgaacctcg acgtccaggt cgcggatggt
     2821 atccgggtga acctcactac ttacctggct tcgaggcacc atgaaaatgt ctgggtgaaa
     2881 ggcggctaca tccaattcga taagctgctc ttcctgcaca gcgccttcgt cgacaatatc
     2941 atgaagagcg ttacgatcaa ggtaggacag ttcgacgtga actatggcga ccaggtattt
     3001 cgcaggacag acggcggcaa caccatctac aatcccttta tcgagaacta catcctggac
     3061 gagtttgcca ccgagatcgg cggcgaggtg atctaccatc ataagagcgg tctgttcgcg
     3121 ctgggtggga ttaccaacgg ggaactggat cccaccgttc tcgggtcttc gaagatcgac
     3181 tctgccaccg gcaaggtgaa cggctatgat gccgcctggc atggcaagat cggttacgac
     3241 aaacagctga cgccggatct ccgggtacgg ctttccggat ccttctatac cgttaaaagc
     3301 gccaatagca acacc
//
LOCUS       DATGFG010000220         5409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1677022, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000220 DATGFG010000000
VERSION     DATGFG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677022"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2988
                     /locus_tag="VKQ52_04020"
     CDS             <1..2988
                     /locus_tag="VKQ52_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ86380.1"
                     /translation="AQPYGGLSPDQMEGFITSYYEYHFLYITGVKYVDSKSIQGAALI
                     KIEFNEGTDMSQAMAEVVGYVNRARAFMPPGTVPPFITRFDAGSVSVGQLVFSSDSRS
                     LSEISDLALFRVRPMFSTLPGVSAPPPFGGNQKTVVVTVDPQKLQGYHLSPDQVVQSI
                     VRFNTITPAGNISIGDSTWMTPQNSVVEDVQDLQKVPLQLGSGPAVYLRDVASVSLGA
                     DITSGYVLINGKRSVYIPVTKRADASTWDVVQRVKAALPDMQAAIPADIKVTYAFDQS
                     GYVIRSLKTVLVEGGLGALFTGLVVLLFLRDVRSALIVIMTIPLALLSAATALYLCGQ
                     TINIMTLGGLALAVGILVDEATVTIENIHRHQETGKDRMRAIADACMEIAGPKLLILL
                     SILAVFVPALFMNGVPRALFLPLSLAVGFAMIASFLLSQTFVPVVSNWVLGKHPVIKT
                     TAFDRMKEKYVRAGQGFHARYALFTLLFIGAALLLAALLFRSTGTQLFPDTDSGQTQV
                     RLRLPVGSRFERTEDATHRLLQLADSIAGKGNVEISSAFVGTQPTSYPVNYIHLWTGG
                     PNESVTKIKLHTGVIPISDFREKLRAASARALPGVRLSFEPGDIVEQVLNLGSNNAVE
                     IAVVNRNLEQGRKTAQSLVQKLSGIPELRDLQIATPLDYPVIKLNVDRIRAGQLGITG
                     DEVARSTVAATSSSRFTAPSYWLDRTTGTAYQVQVQYPSYRMNGTSQLEAVPVSSGQN
                     GQTHYLGEVATWQRGSAPGEYDRLNQQRYITVTANIQQKDRGATYGKIRKVISGMGKL
                     PGGARILLRGQSELLDQTLTSLQFGLVVAIVVIFLAMAVYFQSFRVAVAILSVIPAVV
                     AGALFLLFVSGNTLNIQSYMGTIMAVGVAIANAVLFISQAEQLRRTGTADGNIEAAGH
                     RLRPILMTTTAMIAGMIPMALGLSEGGDQTAPLGIAVIGGLLFSAGSVLFFLPHIYQW
                     LAGRKPYRSLSLYPDDRTPESIH"
     gene            3011..4120
                     /locus_tag="VKQ52_04025"
     CDS             3011..4120
                     /locus_tag="VKQ52_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ86381.1"
                     /translation="MAHISNEFKTLAFVAALILSGACNEPAAPGTGGNAAGAPADTVQ
                     AFLLRTDTLKKSVELPGELLPFYQTDLFAKVQGYVREMRVDIGDRVRKGQTLAVLEAP
                     EVNTQVFQSEAALGAARSKYTASADKYNRLYQASQSPSPGIVAPVDLVASHDQMEADS
                     SAYEALRQQSKAYKEVSGYLYITAPFDGVITVRKADPGALVSASTLLLTVQDNHVLRL
                     RVSVPETYISAATGRRELSFAVDAYPEQRFTGTLTRKTETIDPVTRTELWEYDVDNNR
                     RLLKAGAFVFARLNLERGAPSFVLPASAIATTLERKFVIRVRDGKAQWVDVRQGMTTD
                     AGIEVFGDLHPGDTLLTKATDERKPGTTAYWKFSR"
     gene            complement(4152..4670)
                     /locus_tag="VKQ52_04030"
     CDS             complement(4152..4670)
                     /locus_tag="VKQ52_04030"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ86382.1"
                     /translation="MSTITPLLKEMDQEAQTTRKMLERVPTAYFSWRPHQKSMSIKQL
                     ATHLAELPGWVTMAVTTDGLDFANNPYRQEEVGNTNELLDYFERSLEDGRAHLAAARE
                     ATLAETWTLRNGDDVLHVATKGEIIRMAYCQIVHHRAQLGVFLRLLDIPIPGSYGPSA
                     DEHEVEPSAVEA"
     gene            4820..5083
                     /locus_tag="VKQ52_04035"
     CDS             4820..5083
                     /locus_tag="VKQ52_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86383.1"
                     /translation="MSQQETANPTAPNKKQVRREIGEKLTGALAEYKQELGEKKLATR
                     VKEVSKLISRDLLKISKKQKRDKKTATKAPKKAAKTAKATAKK"
     gene            5203..>5409
                     /locus_tag="VKQ52_04040"
     CDS             5203..>5409
                     /locus_tag="VKQ52_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86384.1"
                     /translation="MASFAELKPNTYYLIQVDAESDIELVSVILLTKETVLLRSYLPQ
                     AEDFFKYQDEPIFKLVEEVDEETAE"
BASE COUNT         1155 a         1582 c         1536 g         1136 t
ORIGIN      
        1 gcccaaccct atggcggcct ctctcccgac cagatggaag gttttattac ttcctattac
       61 gaatatcatt tcctgtatat aaccggtgta aaatatgtcg actccaagag tatccagggg
      121 gcggcactca taaagatcga gttcaacgaa gggaccgata tgagccaggc gatggccgaa
      181 gtagtgggat atgtcaaccg ggcacgggcc tttatgcctc ctggtacagt gccgcccttc
      241 atcacccgct tcgatgcggg cagtgtatcc gtcggtcaat tggtgttcag ttcggacagc
      301 cgttcgctca gcgagatctc cgatctggcg ctcttccggg taaggccgat gttttccacc
      361 ctgcccgggg taagcgcccc accccccttt ggcggcaacc agaagacggt ggtagtgacg
      421 gtagaccccc aaaaactgca ggggtatcat ctctcaccgg accaggtggt acagagcatc
      481 gtccggttca acaccatcac tccggcgggc aatatcagta tcggcgactc aacctggatg
      541 acgcctcaaa atagcgtcgt ggaggatgtg caggacctgc agaaagtgcc gctgcagctg
      601 ggtagcggcc ccgccgtcta tctccgggat gtcgcttcgg taagcctggg ggccgatatt
      661 accagcggct atgtcctcat caacgggaag cgctccgtct atatcccggt gaccaagagg
      721 gccgatgctt ccacctggga tgtcgtgcag cgggtgaagg cggccctgcc cgatatgcag
      781 gccgcgatcc ctgccgacat caaggtcacc tatgcattcg atcagtcggg ctatgtcatc
      841 cgttcgctga aaaccgtact cgtagagggt gggctcggcg ccctgttcac cgggcttgtc
      901 gtcctgctct ttttgcggga tgtccgcagc gccctgatcg tgatcatgac gataccgctg
      961 gccctgcttt cggcggccac cgcgctttat ctctgcgggc agacgatcaa tatcatgacg
     1021 cttggggggt tggcgttggc tgtcgggatc ctggtggatg aggcaacggt caccatcgag
     1081 aatatccacc ggcaccagga gacgggaaag gacagaatgc gtgctatcgc cgatgcctgt
     1141 atggagattg ccggtcccaa gctgttgatc cttctcagca tcctggctgt ttttgtgccg
     1201 gcgttgttca tgaacggggt gccccgggcc ctgttcctgc cgttatccct tgcggtggga
     1261 ttcgccatga tcgcctcctt cctgctttcc caaaccttcg tgcccgtcgt ctcgaactgg
     1321 gtactcggta agcacccggt gataaagacc acggccttcg accgcatgaa agagaagtat
     1381 gtacgggcgg gacaaggttt ccatgcccgc tacgcgctgt ttacgctcct gttcatcggg
     1441 gccgcgctac tgcttgccgc tctgctgttc cggtctaccg gcacccagct ttttcccgat
     1501 accgatagcg gccagacgca ggtgaggctc cgcctgccgg taggctcgcg cttcgagcgg
     1561 accgaagatg ccacccacag gctgcttcaa ttggccgaca gcattgcggg caagggaaat
     1621 gtggagatca gttccgcctt cgtcgggacc cagcccacga gttaccctgt caattatatc
     1681 cacttatgga cgggcggacc gaatgagtcg gtcaccaaga tcaagctgca taccggcgtc
     1741 atccccatct ccgattttcg ggaaaagctt cgggccgcgt cggcaagggc cttaccgggc
     1801 gttcgcctgt ccttcgaacc cggcgatatc gtcgagcagg tgctgaacct ggggagcaat
     1861 aacgccgtgg agatagccgt cgtcaatcga aacctcgaac aggggcgaaa gacggcgcag
     1921 tcgctggtac aaaaactgtc gggcatcccc gagctccggg acctgcagat cgcgacaccc
     1981 ctcgactatc ccgtcatcaa gctgaacgtc gaccggatcc gtgccggcca gctcggcatc
     2041 accggcgatg aagtggcgcg ctctactgtc gccgccacct cctcttcccg tttcacggcc
     2101 cccagctatt ggcttgaccg gacgacgggc accgcttacc aggttcaggt tcaatatccc
     2161 agctatcgca tgaacggcac gtcgcaactg gaagccgtgc cggtatcctc gggtcagaac
     2221 ggccagacgc attacctggg cgaggtagcc acctggcagc gtggctccgc gccgggcgaa
     2281 tatgaccggc tcaatcagca acggtatatc accgtcaccg ccaatatcca acagaaggac
     2341 cggggtgcta cgtatggcaa gatccgcaag gtcatcagcg gcatgggcaa gctgcccggg
     2401 ggcgcccgca tcctgctgcg cggacaaagc gagctgctgg atcagaccct tacgagtctg
     2461 cagttcgggc tcgtcgtcgc catcgtcgtc attttcctgg cgatggccgt ctactttcag
     2521 tccttccggg tggcggtagc catcctgtcg gtgatccccg ccgtggtggc aggcgcgctt
     2581 ttcctgctct ttgtgagcgg taacaccctg aatatccaat cttatatggg gaccatcatg
     2641 gccgtcggcg tcgctatcgc caatgcggtg ttgttcatca gccaggcgga gcagctacgc
     2701 aggacaggga cggcggacgg gaatatcgaa gccgcggggc accggctccg gccgatactg
     2761 atgactacca ccgcgatgat cgccgggatg atcccgatgg ccctcggtct gagtgaaggc
     2821 ggcgaccaaa cggcgccgct ggggattgcc gttatcgggg ggcttctgtt ctccgcgggc
     2881 agcgtccttt tcttcctgcc gcatatctat caatggctgg ccggcaggaa accctatcgc
     2941 agtctgtccc tatatcccga cgatcgtaca ccggaatcca ttcattaaaa agcaacctat
     3001 catacgcaat atggcccata tcagcaacga attcaaaacc ctggcctttg tcgccgcctt
     3061 aatcctgtcc ggcgcctgca acgaaccggc agctccgggg accggcggca acgcggcagg
     3121 cgccccggcg gacaccgtcc aggccttcct gctcaggacg gacaccctga agaaatccgt
     3181 cgaacttccg ggggaactgc tgccttttta tcagaccgac ctgttcgcga aagtgcaggg
     3241 ctatgtccgt gagatgcgag tggacatcgg cgaccgggtt cggaagggac agacccttgc
     3301 cgtgctggaa gcccccgagg taaacaccca ggtattccag tcggaggccg cattaggagc
     3361 ggcaaggtct aaatataccg ccagcgcgga caaatacaac cgcctctacc aggcctccca
     3421 gagtccttct cccgggattg tcgctcccgt cgaccttgtc gcgagccatg atcagatgga
     3481 agccgacagc tccgcctacg aagccctgcg tcagcagtcc aaggcatata aggaagtatc
     3541 gggttacctg tatatcacgg cgcctttcga cggggtgatc acggtccgta aggcggatcc
     3601 cggtgcactg gtaagcgcca gcacccttct cctgacagtt caggacaacc atgtcctccg
     3661 gcttcgggta tccgtccccg aaacctatat atcggcagcc acggggcgtc gtgagttatc
     3721 gttcgccgtc gatgcctatc cggagcagcg ctttacgggg accctcaccc gcaagacgga
     3781 aaccatcgat cctgtaacga ggaccgaact ctgggaatac gatgtcgata acaaccggcg
     3841 ccttctgaag gcgggtgcat tcgtcttcgc ccggctcaac cttgaacggg gggctccttc
     3901 ctttgtattg ccggcttcgg cgatcgccac gaccctcgag cgaaaattcg tcatccgggt
     3961 ccgcgacggc aaggctcaat gggtggatgt cagacaaggg atgacgacgg acgccgggat
     4021 cgaggtcttt ggagacctgc atcccggcga cacgctgctt acaaaggcca ccgacgaacg
     4081 gaagcctggt acgacggcct attggaaatt ttcccgttag atcgtatgcc tcggatctca
     4141 ggcctcggat ctcaggcctc gaccgcggag ggctcgacct cgtgttcatc ggcgctgggg
     4201 ccatagctgc ccgggatggg gatgtccagc agccggagga aaacgcccag ttgtgcgcgg
     4261 tgatggacga tctggcaata ggccatccgg atgatctcac ccttggtagc gacatgaagg
     4321 acgtcatcgc cgttgcgcag ggtccaggtc tctgccaggg ttgcttccct ggcggctgcg
     4381 aggtgggcgc ggccatcttc cagggatctt tcgaaatagt ccagcagctc gtttgtgtta
     4441 ccgacctctt cctggcggta gggattgttg gcaaaatcca gcccatcggt ggttaccgcc
     4501 atggtcaccc aacccggcaa ttccgcaaga tgggtggcta attgtttaat actcatactc
     4561 ttttgatgag ggcgccagga aaaataggca gttgggacgc gttccagcat tttgcgggtg
     4621 gtttgggctt cctggtccat ttcttttaaa agcggagtga tcgttgacat aaaaagtggt
     4681 tttatttgat gtttagtcaa agtaaccacc gggtactgtc agccttatgt cagcagctgt
     4741 caggaaaaac ttaacattgt ccgtattctt tttcatatta aatttaggtt accatattat
     4801 tatcaaaaac attattcgta tgagtcaaca agaaacagcg aatcctacgg ctcccaacaa
     4861 gaagcaggtt cgcagggaga tcggggagaa actgacgggt gcccttgccg agtataaaca
     4921 ggaattgggt gagaagaaac tggctacccg tgtaaaggag gtcagcaaat tgatcagccg
     4981 cgatctgctg aagatcagca agaagcaaaa gcgtgataag aagacggcga cgaaggcgcc
     5041 aaagaaagcc gcaaaaaccg caaaagccac tgcaaaaaaa tagtgccggg ataggcgtat
     5101 cttgtgaccg tccgccctgc gcgggcggtc ttttttttgt gaaaaaccac tcaaccggcg
     5161 cagcatttga ttgttacaaa cacagacaaa aatcacatca ctatggccag cttcgcagaa
     5221 ttaaagccca acacgtatta cctgatacag gtggatgccg aatccgacat cgaacttgta
     5281 tccgtgatcc ttcttacgaa agaaacagtc ctcctgcgca gctatttacc tcaggccgag
     5341 gacttcttca aataccagga cgaacccatt ttcaaactgg tcgaagaagt agacgaagaa
     5401 acggccgaa
//
LOCUS       DATGFG010000221         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_901765, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000221 DATGFG010000000
VERSION     DATGFG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901765"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2093
                     /locus_tag="VKQ52_04045"
     CDS             <1..>2093
                     /locus_tag="VKQ52_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HLZ86385.1"
                     /translation="QRRLQQGQSAILVAGGASINIPRGALRTDAMVSVTALRTIDLPA
                     MDQAMVNVTAGGGGYRFLPHGTKFAHDAKLSLGYDSASIPDGYTVKDIRTYFFDEQKG
                     HWVALPLDSASAGVVHSRTGHFTDMINAVIKTPESPQVEAYNSNSMKGVKAADPSAAV
                     NLIAPPKASSMGGASISYPIEVPAGRAHLQPQLAISYSSGGGDGWLGTGWDLSVPSIG
                     IDTRWGVPRFDAQNETETYSMGGEQLSPVAHRGDLIARTAEKQFFQRVEGAFNRIIRH
                     GNSPQTYWWEITDKEGTRYFYGGDPSTGADAASTLADASGNVAHWALRMTLDLHGNFV
                     HYFYTKVSDPGVSGSSVMGVQLYLSKITYTGSNGTDGRYSVLFGRDRDIDGQTSRKDV
                     TISATNGFKQVTADLLRRIDVQLDGVDIRHYELNYTQGAFYKTLLTGISQYDASGNFF
                     NQHTFDYYNDIASGGTLVPLATAQSWTLGSDNVHGGMLTHLNGFTDEASALSGTSNTD
                     FSGGVTVSVGFGSPTDKLNSIGGSFSYSQSQSKGLLAMVDINGDGLPDKLFYDGASDA
                     LYYRPNLSGATGKTSFGDRLAINGINVFQKDKSSGYTVGLEAVALSALMAGANTGQTT
                     SKTTIYFTEANGDQLPDIVKDGQVYFNHIDPSTGQITFTPTSVGTPSPIFAGVTISQN
                     LVDPTELANDRQQAI"
BASE COUNT          471 a          580 c          624 g          418 t
ORIGIN      
        1 cacaacggcg gttgcaacag ggccagtcgg ccatcttggt tgccggggga gctagtatta
       61 atattccacg tggcgcctta cggacggatg cgatggtcag cgttacagcg cttcgcacca
      121 tcgatctgcc cgcgatggac caggcaatgg tgaatgtgac ggcaggcggt ggtggctacc
      181 ggttcctgcc gcatggcacc aaatttgccc acgatgcaaa gctgagcctg ggatatgatt
      241 cggcgagtat cccggatgga tacacggtga aagatatccg aacctatttt tttgatgagc
      301 aaaagggcca ttgggtggcc ttgcccctgg atagtgcatc ggcgggtgtc gttcactcgc
      361 gcaccggcca tttcaccgat atgatcaatg cggtgatcaa gactcccgag tcgccgcagg
      421 tggaggccta taattccaat tcgatgaagg gcgtcaaggc ggccgacccg agtgccgcag
      481 taaacctcat cgccccgcca aaggctagtt ccatgggggg cgcctccata agctatccca
      541 ttgaagtacc ggcgggccgc gcccatcttc agccgcagtt ggccattagc tacagcagcg
      601 gcggagggga cggctggctt gggacgggct gggatctttc cgtgccttcc atcggcatcg
      661 acacacgctg gggcgtacct cgcttcgacg cgcagaacga gacggaaacc tattcgatgg
      721 gcggcgaaca gctcagcccg gttgcgcacc gcggcgatct gatcgcccgg acggcggaaa
      781 agcaattctt tcaacgggta gaaggggctt tcaacaggat catccggcac ggcaatagtc
      841 cccaaaccta ttggtgggaa atcaccgata aggagggaac aagatatttt tatggtggtg
      901 acccttccac gggtgcggat gctgcaagta cgctggccga tgcaagcggc aatgtcgcgc
      961 actgggcctt gcgcatgacc ctcgacctgc acggcaattt cgtccattat ttctacacaa
     1021 aggtgagcga ccccggcgta agcggcagct ccgtgatggg agtgcagctg tatctcagca
     1081 agatcaccta cacggggtcc aatggtacgg acggacggta ttcggtactg ttcgggcgcg
     1141 accgggatat cgacggacaa accagccgga aggacgtgac catatcggcc acgaacggct
     1201 tcaagcaggt gaccgccgat ctgctgcgac gcatcgatgt gcagctggac ggggtggata
     1261 tccggcatta tgaactcaat tacacgcaag gcgcatttta caagaccctg ctgacgggca
     1321 tcagccagta tgatgcgtca gggaatttct tcaaccagca taccttcgat tattacaatg
     1381 acatcgcttc cggcggcacg ctggttccgt tggccaccgc acaaagctgg acattgggtt
     1441 cggataatgt gcacgggggc atgctgacgc atctcaacgg gtttacggac gaggcctcgg
     1501 cgttgagcgg cacatcgaat accgatttta gcgggggcgt gacggtctcg gtgggttttg
     1561 gcagtcccac cgacaaactg aactccatcg gcggttcctt cagctattcg cagtcgcaaa
     1621 gcaagggatt gctggcgatg gtcgacatca acggagatgg attgccggat aaattgtttt
     1681 atgacggggc cagcgatgct ctttattaca ggccgaacct gtcgggcgcc acaggcaaga
     1741 ccagctttgg agacaggctc gcgatcaatg gcatcaatgt ctttcagaaa gataagtcct
     1801 ccggctatac ggtaggattg gaagccgttg cgctgtcggc cctgatggcc ggggccaata
     1861 ccggccagac gaccagcaag acgacgattt atttcacaga ggccaatggt gaccagctgc
     1921 ccgatatcgt aaaggacggc caggtgtatt tcaatcatat cgatccgtcg acgggccaga
     1981 tcaccttcac accgaccagc gtaggtacac ccagccccat cttcgccggg gtaacgatca
     2041 gccaaaacct ggtagacccc accgaactgg cgaatgatcg ccagcaggcc atc
//
LOCUS       DATGFG010000222         4257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_761805, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000222 DATGFG010000000
VERSION     DATGFG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761805"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VKQ52_04050"
     CDS             <1..370
                     /locus_tag="VKQ52_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86386.1"
                     /translation="VTKVVNAYVDFLTEFPDLPLFILNEIQGNPTQLAAKLNAEVGPA
                     RRQFFQQLTEAKKEGKTALDPFHFVANLVGLTVFPFVAKPLLTRVTNVNDEQFRAYME
                     ERRRLVPMWIGMMMRAGAGQ"
     gene            500..1825
                     /locus_tag="VKQ52_04055"
     CDS             500..1825
                     /locus_tag="VKQ52_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ86387.1"
                     /translation="MKKRGIILLGLLLPGLLGLAQQRDQLTLEQCYQLGEANYPLTRQ
                     RELIGRTRDYTIANIAKGIYPQLAVSGSATYQSDVTKIAIPPIGGINVNIPTVPKDQY
                     KLYGEVSQTLTDFGINKQRRVISRTDAELQDANLSADLYALRDRINQLFFGVLLIDGQ
                     LEQNELAAQDIRTGIARVQAAVKNGTDFTSSLNKLRAELLKTEQHSVELRASRSAYTD
                     MLGLFVNRPLDSMTVLVRPEAPVAGNAGGVAGNVGAGLPSADTIRRPELKAYDLQVQS
                     YAEQLRLTKLNLFPSVSAFFQGGVGQPNPMNFLSTSLSGYYLTGLRLTWTIGGSYTYK
                     KDRLISQNNQDIVKAQRSTFLFNTSLTMQQENADIRKYRALVQSDEEIVRLRESVGKT
                     SAVQLENGVISANDYLLDVNATAQARQDRVVHEMELLQAQYNYKTTSGN"
     gene            1839..2771
                     /locus_tag="VKQ52_04060"
     CDS             1839..2771
                     /locus_tag="VKQ52_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ86388.1"
                     /translation="MRKQFVLGLFVFAFAACGQHKIKTDASGVFESDEVIVSAEQNGK
                     ILSFPIMEGDTLAKNAIIGQIDMSNTVIQKQQIQATITALKEKTTNPRPQIELVQRQL
                     AVEESQLAQQLREKTRTENLLKADAATQKQLDDITSSIDQLQKQIAVSKQQIALDNSN
                     IATQNRTIFSEQAPLEMSAKQIQDNIDKGRIINPIRGTVLTKYALQGEMTTTGKALYK
                     IANLDTVTLRAYVTGSQLTQVKLGQTVKVYSDEGADQYREYAGQVSFISDKSEFTPKT
                     IQTRDERANLVYAVKIRVKNDGFLKLGMYGEVKF"
     gene            2774..3676
                     /locus_tag="VKQ52_04065"
     CDS             2774..3676
                     /locus_tag="VKQ52_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ86389.1"
                     /translation="MISLSAITKTYNKGQVLAVKDISFDVRPGELFGLIGPDGAGKTT
                     LFRILTTLLLADKGSATVNGFDVVKDYTKIRNSVGYMPGRFSLYQDLTIEENLHFFAT
                     VFGTTIRENYALIKDIYQQIEPFKDRRAGKLSGGMKQKLALCCALIHKPAVLFLDEPT
                     TGVDVVSRKEFWDMLGRLKSEGITILVSTPYMDEARLCDRIALIQKGSILSIDTPEGI
                     VGQFPGALFGVQAGNTHQLLREVRAYEGTETCFAFGDALHVTFRGGAADGLLPYLREK
                     GLGDARVGLITPGIEDCFIHLLKN"
     gene            3679..>4257
                     /locus_tag="VKQ52_04070"
     CDS             3679..>4257
                     /locus_tag="VKQ52_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ86390.1"
                     /translation="MDTVITTEKLTKRFGAFIACNELSFSVSKGEIFGFLGANGAGKT
                     TAMRMLCGLLAPSSGKASIAGFDVYSQAERIKRCIGYMSQKFSLYDDLSVAENIRFYA
                     GIYGMSRPAIRKKTGQLLEELQLQSEAKTLVRELPLGWKQKLSFSVSIVHDPQIVFLD
                     EPTGGVDPVTRRQFWDLIYAAADKGVTVFVTTH"
BASE COUNT          974 a         1049 c         1371 g          863 t
ORIGIN      
        1 ggtgacaaag gtggtgaatg cctatgtcga ttttcttacc gagttcccgg acctgccgct
       61 ttttatcctc aacgagatac agggtaaccc aacgcagctg gcggccaaac tcaacgcgga
      121 agtagggccg gcaaggcggc agttctttca gcagctgacg gaggcgaaga aggagggcaa
      181 gacggcgctg gatcctttcc atttcgtcgc caacctggtg gggctaacgg tattcccgtt
      241 tgtagccaag ccgcttttga cgcgggtgac gaatgtgaat gacgagcagt tcagggcata
      301 tatggaggag cggcggcggc tggtgccgat gtggatcggg atgatgatgc gggccggtgc
      361 gggccagtga tgccgggcgt gggggctggt gatgcaaggg gtgcggcctg gagatgcaaa
      421 ggtcggcggc cggtgacgtt tttggccgga attttttttg gcccgcggtt aatcaaatga
      481 ttaaaacaaa attataaaca tgaaaaaaag agggatcatc ctattggggc tgcttttgcc
      541 gggtcttttg ggtctggccc agcagcggga tcagttgaca ctggagcagt gctatcaatt
      601 gggcgaggcc aattacccgc ttacccgtca gcgggagctg atcggaagga cgagggatta
      661 taccatcgcc aatatcgcca aggggatcta cccccaatta gcggtaagcg gttcggcgac
      721 ctaccagtcc gatgtgacga agatcgccat cccgccgatc ggggggatca atgtcaatat
      781 cccgacggtg cccaaggacc agtacaagct ctatggcgag gtatcgcaga cgctgaccga
      841 tttcgggatc aacaagcagc gtcgcgtgat cagccgcacc gatgccgagt tgcaggatgc
      901 gaacctgtcg gccgatctgt atgcgctgag ggaccggatc aaccaactct tcttcggggt
      961 cctgctgatc gacggacagc tggagcagaa tgaactcgcg gcgcaggata tccggacagg
     1021 tatcgcccgg gttcaggcgg cggtgaagaa cgggacggat tttaccagca gcctgaacaa
     1081 gttaagggcg gagttgctga agaccgaaca gcacagtgtc gaactacggg cttcgcggtc
     1141 ggcgtatacg gatatgctgg ggttgttcgt caaccggccg ctcgacagta tgacggtgct
     1201 ggtgcgcccg gaagcgcccg tcgcgggcaa tgccggcgga gttgcgggca atgttggcgc
     1261 gggtctgccc tccgcggata cgatccggcg tccggagcta aaggcctatg acctgcaggt
     1321 gcagtcttat gcggagcagt tgcggctgac gaagctcaat cttttcccgt ccgtaagcgc
     1381 tttcttccag ggcggtgtcg gccagcccaa cccgatgaat ttcctgtcca cctcgctgag
     1441 cggttattat ctcaccgggt tgcggctgac ctggaccatc ggcgggagct atacctataa
     1501 gaaggaccgg ctgatcagcc agaataacca ggacatagtg aaggcgcagc gcagcacctt
     1561 cctcttcaat acctcgttga cgatgcagca ggagaacgcc gatatccgga aataccgggc
     1621 gttggtccag agcgatgagg agatcgtgcg gttacgggag tcggtgggga agacttcggc
     1681 cgttcagctc gagaacgggg tgatcagcgc aaatgattat ctcctcgacg tcaacgcaac
     1741 ggcgcaggcc cggcaggacc gggtcgtcca tgaaatggaa ttgctgcagg cgcagtataa
     1801 ctataaaaca acatcgggaa actgacaaaa ttaagtatat gagaaagcaa tttgttctcg
     1861 gcctttttgt attcgcgttc gcggcctgcg ggcagcacaa gatcaaaacg gatgcttccg
     1921 gagtcttcga gtcggacgag gtgatcgtat cggccgagca gaacgggaag atcctctcct
     1981 tccctatcat ggagggcgat accctggcga agaacgccat catcgggcag atcgatatgt
     2041 ctaataccgt tatacagaaa cagcagatcc aggcgacgat caccgcgttg aaggagaaga
     2101 cgactaatcc ccggccgcag atcgaactgg tgcagcggca gctggcggtg gaagaatcgc
     2161 aattggcgca gcagttaagg gaaaagacgc gcaccgaaaa tttgttgaag gccgatgcgg
     2221 cgacgcagaa gcaactggat gacatcactt cgtcgatcga ccagttacag aaacaaatag
     2281 cggtgtccaa acaacagatc gcgctggaca attcgaatat cgccacgcag aaccggacca
     2341 tcttcagcga gcaggcgccg ctggaaatgt cggccaaaca gatccaggac aatatcgaca
     2401 agggcaggat catcaacccg atacggggga ccgtgctgac gaagtatgcg ttgcaggggg
     2461 agatgacgac gacggggaag gccctgtata agatcgccaa tcttgacacg gtgaccctgc
     2521 gggcctatgt caccgggtcg caattgaccc aggtgaagct ggggcagacg gtgaaggtgt
     2581 attcggatga gggggcggat caataccggg agtacgcggg gcaggtgtcc tttatctcgg
     2641 acaaatccga gtttacccca aagaccatcc agacccggga cgagcgcgcc aacctcgtgt
     2701 atgcggtgaa gatccgggta aagaatgacg gtttcctgaa actggggatg tacggtgaag
     2761 taaagtttta accatgatca gtctatcagc cattacaaag acctataaca aggggcaggt
     2821 gctcgcggtg aaggatatct ccttcgacgt gcggcccggc gagctgttcg ggcttatcgg
     2881 tcccgacggt gcggggaaga cgacgctgtt ccgtatcctg accacgctgc tgctggcgga
     2941 caagggatct gcgacggtga atggcttcga tgtagtgaaa gactacacga agatccggaa
     3001 cagcgtgggc tatatgccgg ggcggttcag tctttaccag gacctgacca tcgaagagaa
     3061 cctgcatttt tttgcgacgg tgttcggtac gaccatccgc gagaactatg cattgataaa
     3121 agatatttat cagcagatcg aaccttttaa ggaccgccgc gccgggaaac tatcgggtgg
     3181 catgaaacaa aaactggcat tgtgttgtgc attgattcat aagccggcgg ttctttttct
     3241 cgatgagccc acgacggggg tggatgtggt atcccggaag gaattttggg atatgctcgg
     3301 gcggttgaag tccgaaggca tcaccatttt ggtctccacc ccctatatgg acgaggcccg
     3361 gctttgcgac cggatcgcgc tgatccaaaa ggggagtatc ttatcgatcg acaccccgga
     3421 ggggatcgtt gggcaattcc cgggtgcgtt gtttggagta caggcgggga atacgcacca
     3481 gttgttgcgg gaggttcgcg cctatgaagg gacggaaacc tgttttgcct ttggggatgc
     3541 gttgcatgtg accttcaggg gcggggcggc ggatgggctg ttgccttatc tgcgggagaa
     3601 gggactgggg gatgcgcggg tcgggctgat cactccgggc atcgaggatt gttttattca
     3661 tcttttaaag aattgagcat ggacacggtg atcacgacgg agaagctgac caaacgattc
     3721 ggggccttta tcgcgtgcaa cgaactgagc ttctccgtga gcaagggaga gatcttcggg
     3781 tttctcggcg ccaacggggc ggggaaaacg acggcgatgc ggatgctttg cgggctgttg
     3841 gcgccctcct cggggaaggc cagcatcgcg ggtttcgacg tgtattcgca ggccgagcgg
     3901 atcaagcggt gcatcgggta tatgagccag aagttcagcc tgtatgatga cctctcggtg
     3961 gcggagaata tccggttcta cgccggcatc tatgggatga gcaggccggc gatccggaag
     4021 aagacggggc agttgctgga agagctgcaa ttgcagagcg aggcaaagac gctggtacgg
     4081 gagctgccgc tcggctggaa acaaaagttg tcgttctcgg tatcgatcgt gcatgatccg
     4141 cagatcgttt ttttggacga accgacgggc ggggtcgatc ccgtgacacg gcggcagttc
     4201 tgggacctga tctatgcggc ggccgacaag ggggtcacgg tctttgtcac aacccac
//
LOCUS       DATGFG010000223         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_309035, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000223 DATGFG010000000
VERSION     DATGFG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309035"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VKQ52_04075"
     CDS             complement(<1..240)
                     /locus_tag="VKQ52_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLZ86391.1"
                     /translation="MSSVLFEIRDQIAFITLNRPDKMNAFTREMALLLQSRLDECASL
                     HEVRAVYLTGAGKAFSAGQDLAEVVDPNGPGMAKIL"
     gene            complement(243..722)
                     /gene="paaD"
                     /locus_tag="VKQ52_04080"
     CDS             complement(243..722)
                     /gene="paaD"
                     /locus_tag="VKQ52_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HLZ86392.1"
                     /translation="MKTIWSLLETVNDPEVPVLSVVDLGIIRDVRMAGGHPEIVLTPT
                     YSGCPAMDMIRMQIRMVLLEHGFTDTRLTTVLSPAWTTDWMSEKGKEKLVAYGIAPPL
                     PVQTVCHPALFQREEAIACPRCHSYHTTLISEFGSTACKALYRCEDCHEPFDYFKCH"
     gene            complement(735..1538)
                     /gene="paaC"
                     /locus_tag="VKQ52_04085"
     CDS             complement(735..1538)
                     /gene="paaC"
                     /locus_tag="VKQ52_04085"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HLZ86393.1"
                     /translation="MTTQHTIDYALHLADNALVLGHRNSEWTGHGPILEQDIALSNIA
                     LDLIGQARYFYQYAARLMNAAARPGEPEVTEDVLAYLRDSWDFRNCLLVEQANGDWGK
                     TILRQFFFSAYQYYFYKELQRSRDGELSAIAEKALKEVTYHLRWSSEWVIRLGDGTEE
                     SHSRMRKALDELWMYTGELFQPADYERSLIPVGVAVDLAEIRPLWEERVREVLKEAKL
                     VHPGSMRADGKATKEWMQGGGKQGRHTEQLGYLLAEMQFLQRAYPACEW"
     gene            complement(1540..1830)
                     /gene="paaB"
                     /locus_tag="VKQ52_04090"
     CDS             complement(1540..1830)
                     /gene="paaB"
                     /locus_tag="VKQ52_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HLZ86394.1"
                     /translation="MEKQHNWPLWEIFIRSKQGLDHKHVGSLHAADAAMAIENARDVY
                     TRRMEGVSIWVVESRHIHASNPDEASSFYDPANDKVYRHPTFYDLPDELKHM"
     gene            complement(1814..2752)
                     /gene="paaA"
                     /locus_tag="VKQ52_04095"
     CDS             complement(1814..2752)
                     /gene="paaA"
                     /locus_tag="VKQ52_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HLZ86395.1"
                     /translation="MTAASETIFQDKIDQDIRIEPKDWMPEAYRKTLIRQISQHAHSE
                     IVGMLPEANWITRAPSLRRKATLIAKVQDEAGHGLYLYSAAETLGISREEMIGQLHSG
                     KAKYSSIFNYPTITWADMGAIGWLVDGAAIMNQVPLCRASYGPYARAMVRICKEESFH
                     QRQGFEILLVLSRGSAAQRRMCQDAINRWWWPSVMMFGPNDDASPNTAQSMQWKIKRF
                     TNDELRQRFIDVCAGQVKLLGMTLPDDQLRWNEERQHYDFGPIDWEEFRNVIDGNGPC
                     NKERLAARVQAHEDGAWVREAALAHAQKKIQYGKTA"
     gene            2878..3477
                     /locus_tag="VKQ52_04100"
     CDS             2878..3477
                     /locus_tag="VKQ52_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HLZ86396.1"
                     /translation="MSLLARVRNILLSPLAEWRIIATERTTIKSLLGRYVVPMALVPA
                     VASFIGYGLVGANGILFRMSGPYWGVSMAMNSFITSLAVYFLGTFLIDRLGPGFGASR
                     DLGRSAQLVAYSYTPAWLAGVFYALPGLQQGVVLGLYSVYLFYTGVPSLKTIPEDQRI
                     TYTIVSAILLITIRFLVGLLLSDLIYSFASAPFIWSQSQ"
     gene            complement(3455..>3764)
                     /locus_tag="VKQ52_04105"
     CDS             complement(3455..>3764)
                     /locus_tag="VKQ52_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLZ86397.1"
                     /translation="DDAGLLKDKVSQVTVRLDGISHVFDLPFDGDSVLNAALMEGVDL
                     PFACKGGVCCTCRAKLIEGEVEMDVNYALEADELSAGFILTCQSHPRTEKVVIDFDSK
                     "
BASE COUNT          783 a         1206 c          928 g          847 t
ORIGIN      
        1 aaggatcttc gccatccccg gcccattcgg atcgaccacc tccgccaggt cctggcccgc
       61 ggaaaaagcc ttaccggcgc ccgtcaggta gacggccctc acctcatgca gactggcgca
      121 ctcatccagg cggctctgta agagcagcgc catctcgcgg gtaaaggcat tcattttatc
      181 aggtctgttc agggtaataa aagcgatctg gtccctgatc tcgaatagaa cggaagacat
      241 attcaatgac atttaaaata atcgaagggt tcatgacaat cctcgcagcg atacaacgcc
      301 ttgcaggccg tactgccgaa ctcactgatc agtgtagtat ggtaagaatg acaacgggga
      361 caggcgatcg cttcctcccg ctggaacaac gccggatgac atacggtctg gaccggaagc
      421 ggcggggcga taccataagc caccagtttc tcctttccct tctcactcat ccagtccgtt
      481 gtccaggccg gcgaaaggac ggtggtcagc cgggtatccg taaacccatg ctccaaaagg
      541 accatccgga tctgcatccg gatcatatcc atcgccgggc agccactata cgtaggcgtg
      601 agcacgatct ccgggtggcc tcccgccatc cggacgtccc ggatgatgcc gaggtctacc
      661 accgacagca ccggaacctc ggggtcgttc accgtttcca acagggacca tatcgtcttc
      721 acctcatcca tattctacca ttcacaggca gggtaggccc tttgaagaaa ttgcatctcc
      781 gcaagcaggt aacccagttg ttcggtatgc cttccctgct ttccacctcc ctgcatccat
      841 tcctttgtgg cctttccatc ggcccgcatc gaccccggat ggaccagctt cgcctccttc
      901 aacacctcgc gtacccgctc ctcccacaac ggccggatct ccgccagatc gaccgcaaca
      961 cccaccggta tcagcgatcg ctcatagtcg gccggctgaa aaagctcccc ggtatacatc
     1021 cacagctcat cgagggcctt tctcatccgg gaatgactct cctccgtccc gtcccccaac
     1081 cggatcaccc attcgctgct ccagcgaaga tgataggtca cttccttcag ggccttctcc
     1141 gcgatggccg acaactcgcc atcccggctt cgttgcagtt ctttataaaa ataatattga
     1201 taagcgctga agaagaattg ccgcaggatc gtcttgcccc agtcgccatt ggcctgctcc
     1261 accagcagac agttccggaa atcccaggaa tcccgcagat aggccaggac atcctccgtt
     1321 acctccggct cccccggtct cgccgccgca ttcatcagcc gcgccgcata ctggtagaaa
     1381 taacgcgcct ggccgatcag gtccaatgca atgttggaca gggcgatgtc ctgctcgagg
     1441 atgggtccat gaccggtcca ttcactgttg cgatgaccaa ggacaagcgc attgtcggca
     1501 aggtgtaagg cataatcgat ggtgtgttga gtggtcatct tacatatgct ttaattcatc
     1561 cggcagatcg taaaaggtcg ggtgccggta gaccttatcg tttgcaggat cgtaaaaaga
     1621 gcttgcttca tccgggttgg acgcatggat atgccggctc tccacaaccc agatgctgac
     1681 gccttccatc cggcgcgtat agacatcccg ggcattctcg atagccatgg ccgcatcggc
     1741 ggcatgaagg ctgcccacat gtttgtgatc caaaccctgt ttgctacgga taaagatctc
     1801 ccaaagaggc caattatgct gtttttccat attgtatctt tttctgcgca tgcgccagcg
     1861 ccgcctcccg cacccaggcc ccatcctcat gcgcctgcac ccgcgccgcc agccgttcct
     1921 tgttgcaggg gccgttgccg tcgatgacat tccgaaactc ctcccagtcg atgggcccaa
     1981 aatcatagtg ctgccgctct tcattccagc gaagctggtc atcgggcagc gtcatcccca
     2041 gcagcttcac ctgcccggcg cagacatcga taaaccgctg ccgtaactcg tcattggtga
     2101 accgcttgat cttccactgc atactctgcg ccgtattcgg cgaggcatcg tcattgggcc
     2161 caaacatcat caccgaaggc caccaccagc ggttgatggc atcttggcac atccgtcgct
     2221 gcgccgcgct gccccggctc agcacaagca ggatctcgaa tccctgccgc tgatgaaagc
     2281 tttcctcctt gcagatccgc accatggccc gggcataggg cccataggag gcccggcata
     2341 agggaacctg gttcattatg gccgcaccat cgacaagcca gccgatggcg cccatatccg
     2401 cccaggtgat cgtcggataa ttgaagatgg acgaatactt cgcctttccg ctatgcaact
     2461 gcccgatcat ctcttcccgg ctgatcccaa gcgtctctgc cgccgaatac aggtacaacc
     2521 catgccccgc ctcatcctga accttggcga taagcgtagc cttccgcctg agcgacggcg
     2581 cccgggtgat ccagttggcc tccggtaaca tacccacgat ctcgctatgc gcatgctgcg
     2641 agatctgcct gatcagggtc tttcggtaag cctccggcat ccaatccttt ggctcgatcc
     2701 ggatatcctg gtcgatcttg tcctggaaga tggtttcact tgcggcggtc atacaacatt
     2761 ggtttatatt gcaaaagtac cctttttttt aaaagctaac aactgttagc aagccttgga
     2821 cgcttttaaa ttagtatggt ataattagta tcttagaagg gtttaattcc attcaccatg
     2881 tcccttcttg ccagggttag gaacatactc ctgtcgccgc tcgccgaatg gcggatcatc
     2941 gcgacggaga gaacgaccat aaagtcgctg ttgggaagat acgtggttcc catggccctg
     3001 gtccccgccg tcgccagctt tatcggctat ggcctcgtcg gcgccaacgg catcctgttc
     3061 cggatgagcg ggccctattg gggcgtctcc atggcaatga acagcttcat cacctccctg
     3121 gccgtctatt tcctgggaac cttcctgatc gaccgcctgg ggcccggctt cggggcatcg
     3181 cgggatcttg gccgctcggc gcaactggtg gcctattcct atacccccgc ctggctggcc
     3241 ggggtctttt acgcactccc cggattacaa cagggggtgg tattgggctt gtatagcgta
     3301 tatctcttct ataccggtgt cccaagcctg aagacgatac ccgaggacca gcgcatcacc
     3361 tatacgatcg tcagcgccat cctgctgatc accattcgtt tcctggtggg attgctgctt
     3421 tccgatctca tctattcttt tgcgtcggcc ccctttattt ggagtcaaag tcaatgacca
     3481 ccttctccgt ccgcggatga gactggcagg tgaggatgaa ccccgcggaa agctcatcgg
     3541 cctccaacgc atagttgaca tccatctcca cctctccttc gatcagtttg gcccggcagg
     3601 tgcaacagac accacccttg cacgcaaagg gcagatcgac cccttccatc agggccgcat
     3661 tcagcacgga gtcgccgtca aaaggcagat caaaaacatg gctgatgcca tccagccgga
     3721 ccgttacctg gctgaccttg tccttcagca gacccgcatc gtcc
//
LOCUS       DATGFG010000224         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_560340, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000224 DATGFG010000000
VERSION     DATGFG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560340"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="VKQ52_04110"
     CDS             <1..1582
                     /locus_tag="VKQ52_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HLZ86398.1"
                     /translation="GSTTTYQQAGIRKNDLSGNYTLGWDYEADKRNFMTASLRYNPLN
                     SKYYQDNLRTDSYPGAVPDSSLLNQVEVTGRSGTVDMSFDYTHVLGRPQREFSFLTLF
                     SRTDRRNGFSSVQQNPADESFIGGIRNDNRSWNQEITLQADYQTPIDSGQLLDLGAKY
                     IIREVVSNYDYLSAAGNGKYLPDLSPTLTNRFIYHQHVTAGYIEYTLTTKSAYSFRAG
                     ARYEFTAIDAQLPNIAASATGIPSYGVLAPGIIIARRLKNGKTIKLAYNRRIQRPSIQ
                     YLNPNVIASNPLSITTGNPTLGPEYSDNVEIGYNTAIRRTTLSFSGFYRRTTRAIESL
                     SLPGAGGDTIERTYGNIGKESTGGVNVFANLEIGRKVSMSGGVDLYYTVLDNAPPLAD
                     SADANFAGHNKGWVFSGRLSGGYSFAKGWSLYIYSVYRGRQVQLQGYQSGLPYYSLTL
                     RREFLKKKGTIGLGAENFLSPGISVKNYVRSAALSQQTTNLTHTLSFRLNLSFRIGKL
                     TVEKAERKKKSITNDDLK"
     gene            complement(1637..>2570)
                     /locus_tag="VKQ52_04115"
     CDS             complement(1637..>2570)
                     /locus_tag="VKQ52_04115"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HLZ86399.1"
                     /translation="LLSDNYDNIKRYVDHISELYPDGLTDWGLGDWAPLRSRSPVELT
                     SSAYYYTDVTILSKAAKRLNKAGDFEKYAALAEKIKTAINKKYLHPEKAIYADGHQTE
                     QSVPLFWGIVPDSLKARVAANLADSIAANNYHLDVGILGAKAVLSALSDNGQAQTAWR
                     LASQTTFPSWGWWIVNGATTLYENWNIDAQRDLSMNHIMFGEIGAWLFKGIGGIKPDE
                     NAPGFKHIWFSPQFVEGLDHFTAEHTGPYGNIISSWKREAGVIKYTVTIPANSTATII
                     FPLHASQSVRYQGKPVANPGNYILSSGVYEFEIR"
BASE COUNT          580 a          696 c          733 g          561 t
ORIGIN      
        1 ggggtccacg accacatatc aacaggcggg catccgcaag aacgacctct ccggtaatta
       61 tacccttggg tgggactatg aagccgacaa aagaaatttc atgaccgcct ccctgcggta
      121 caacccgctc aacagcaaat attatcagga taatcttcga acggatagtt accccggcgc
      181 cgtcccggac agctcgcttt tgaaccaggt ggaggtaacg gggagatcgg gcaccgtcga
      241 tatgagtttc gactataccc atgttcttgg caggccgcaa cgggagttca gttttctcac
      301 gctctttagc cgaaccgatc gccggaacgg gttctcgagt gtgcagcaaa accccgcgga
      361 tgagtcgttt atcggcggga tcaggaatga taacaggagt tggaaccagg agatcacctt
      421 acaggcggat tatcaaacac ccatcgactc cggtcaactc ctcgatctcg gggcgaagta
      481 tatcatccgg gaagtggtca gtaattatga ctacctgagc gccgcgggta atggcaaata
      541 cctgccggac ctgtccccca ccctgaccaa ccggttcatt tatcaccagc atgtaacggc
      601 gggatatatc gagtataccc tgactaccaa atccgcttat agtttccggg ccggggcgcg
      661 atacgagttt accgcgatcg acgctcagct gcccaatatt gcggcatccg caaccggcat
      721 cccgtcgtat ggcgttcttg caccgggaat catcatcgcc aggcgcctga aaaatggaaa
      781 gacgatcaaa ctggcgtaca accggagaat tcaacggccc tccattcaat acctcaatcc
      841 taacgtgatc gcttccaacc cgctgagcat tacgaccggc aatcccaccc tgggcccgga
      901 atactcggac aatgtcgaga tcggctataa tacggcgata cggcgaacga cccttagttt
      961 ttccgggttc tatcgccgta cgacaagggc gatcgagagc ctgagccttc ccggcgccgg
     1021 cggcgacacc atcgagagga cctatgggaa tatcggaaaa gagtccaccg gcggggtcaa
     1081 tgttttcgcc aacctggaga tcgggcggaa ggtctcgatg agcgggggcg tggacctgta
     1141 ctatacggtg ctggataatg ctccgccttt ggcggattcc gccgatgcca attttgccgg
     1201 ccataacaaa ggctgggtat tcagcggcag gctgtccggg ggttatagtt tcgcaaaagg
     1261 atggtccctc tatatttaca gcgtctacag gggcaggcag gtgcagttgc agggatatca
     1321 aagcggtctt ccctactata gccttaccct gcgacgggag ttcctgaaga aaaaaggaac
     1381 gatcgggctt ggagcggaga acttcctgtc ccccggtatt tcggtcaaaa actatgtacg
     1441 gagcgcagcg ctgtcccagc agaccaccaa ccttacccat acgctgagtt tccggctgaa
     1501 cctgagtttc cggatcggga agctgacggt ggagaaggcg gaacggaaga agaagtccat
     1561 taccaacgat gacctgaaat aggggcggca gcgggagcgg cgggtggcgt ggcaggcgac
     1621 ggtggcggcg gcgggtttac ctgatctcga attcatagac gcccgaggac aggatgtaat
     1681 tgcccggatt tgcgaccggc ttgccctggt agcggaccga ctgggaggcg tggaggggga
     1741 agatgatggt tgccgttgag ttggcaggga tcgtcacggt atattttata acgccggctt
     1801 cgcgtttcca ggacgagata atattcccat aggggcccgt gtgttccgcc gtgaaatgat
     1861 ccagcccttc gacgaattgg ggcgagaacc aaatgtgttt gaaacccggg gcattctcat
     1921 cgggctttat gccgccgatg cccttgaaga gccaggcgcc gatctcgccg aacatgatat
     1981 gattcatcga caggtcgcgc tgcgcgtcga tgttccaatt ctcatacagg gttgtcgccc
     2041 catttacgat ccaccagccc caggagggaa aggtggtttg cgaggcgagg cgccaggccg
     2101 tctgtgcctg gccgttatca ctcagagcgc tcaggaccgc cttggcaccc aggatgccca
     2161 catccagatg gtagttattg gcggctatcg agtcggcgag attcgccgct actcttgcct
     2221 tgagactgtc cggcacgata ccccaaaata acggtacgct ttgctctgtc tggtggccat
     2281 ccgcgtaaat ggccttttcc ggatgcaggt attttttgtt gatcgccgtc tttatctttt
     2341 cggccaatgc ggcgtatttt tcgaaatcgc cggccttatt caagcgtttg gctgcttttg
     2401 acaggatggt gacatccgta taatagtaag cggaagaggt gagttcgacc ggtgatctcg
     2461 acctcaacgg cgcccagtca cccagtcccc agtccgtcag gccatcggga tacagctcgc
     2521 tgatatgatc gacgtagcgt ttgatattgt catagttgtc gcttaacaat
//
LOCUS       DATGFG010000225         6375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1446972, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000225 DATGFG010000000
VERSION     DATGFG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446972"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VKQ52_04120"
     CDS             complement(<1..282)
                     /locus_tag="VKQ52_04120"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HLZ86400.1"
                     /translation="MTTFSFQTSRFSRYFFLRTEIDRLLTASIFFSCLLVAIRILHTG
                     STLFIMMIWNLFLAYIPYALSSWLTKKPQRRRLVTIAVSIIWLLFIPNSF"
     gene            complement(279..647)
                     /locus_tag="VKQ52_04125"
     CDS             complement(279..647)
                     /locus_tag="VKQ52_04125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HLZ86401.1"
                     /translation="MQQEKFSIRSRLLSFTHAIRGIRQFVWHEHNARIHLVATIAVII
                     TGWLLKVSLPEAAILAAVTGLVWVAEILNTCIEKLADHITKERHPEIKHIKDLAAGAV
                     LTAAAIAVIAGLLIFIPKII"
     gene            complement(674..2068)
                     /locus_tag="VKQ52_04130"
     CDS             complement(674..2068)
                     /locus_tag="VKQ52_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLZ86402.1"
                     /translation="MKIKMILPALTEAKSPFWRPIKYSLFPPLGLATLAAYCSPEDEI
                     DLQDEHVEELNLDDEPRLVIIQVYITNAFRAYAIADHYREKGAYVCLGGLHVTSLPDE
                     AAAHADSIFIGPGEDTFPQFLRDLRNNKPQKRYSNRERDLTSIPPIRRDLIKRNRYLV
                     PNSIVVSRGCPHHCDFCYKDAFFEGGRSFYTQQVDAALAEIDRLPGRHLYFLDDHLLG
                     HQRFATALFDGMKGMDRVFQGASTIDAILRGDLIESAAAAGMRSVFVGFETLSRNNLL
                     QGNKKQNIGQDYEEAIRRLHSLGIMINGSFVFGLDADDPDVFKRTVDWAVKNSLTTCT
                     FHILTPYPGTRLFREMESQGRILHRNWDQYDTRQVVYRTIGLSAAELKKGYDFAYRSF
                     YSWSNIAKASLGHDHLKHAIKHFAYAGGWKKFEPVWNFMIKTQGLNKMLPLLEAILSK
                     VKRQSTTINLPAIA"
     gene            complement(2079..2468)
                     /locus_tag="VKQ52_04135"
     CDS             complement(2079..2468)
                     /locus_tag="VKQ52_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86403.1"
                     /translation="MTRSPFLRPLASGILGITLMIPACYFLLTLLVRICFGAKTMYYN
                     IAPSFLQSPFDLFALHKAQLILGSLLLAVLFNVAAIIKLRLHHGPRGLELGVAYRRYW
                     LNTAIAIQSILLLVVLLAYTLIQHIRY"
     gene            complement(2550..2855)
                     /locus_tag="VKQ52_04140"
     CDS             complement(2550..2855)
                     /locus_tag="VKQ52_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HLZ86404.1"
                     /translation="MKNPIGNLNKVFDSRIRMGVMSILLVNEEVSFNDLKQLLELTDG
                     NLASHLINLEENGFIKVHKGFIGRKTNTTYSITKAGEKAFKDHLEALENMIRGVNKA"
     gene            complement(2852..3499)
                     /locus_tag="VKQ52_04145"
     CDS             complement(2852..3499)
                     /locus_tag="VKQ52_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86405.1"
                     /translation="MTNQSPTEQSLETLQDIKRMMERSSRFISLSGLSGVSAGVCALI
                     GAWIARGWIRSYVSSVDTAAPERWPAEARDWFELRMILLALGVLIAALATSTWFTWRK
                     ARRSKLPIWDHASKKLAVNMAIPLAAGGFFVLGLLDHSGWNYVAPSCLVFYGLALVNA
                     SKYTLTDIRYLGLLEIALGCVNMYYPHEGLYFWAAGFGVLHIIYGLIMWWKYERS"
     gene            complement(3777..4721)
                     /locus_tag="VKQ52_04150"
     CDS             complement(3777..4721)
                     /locus_tag="VKQ52_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HLZ86406.1"
                     /translation="MDFYDRTGKMALGSRLRRLSEQMTEQAAAIYQLYQVDLQPKWFP
                     VFYSLSPAEARSITDIAREIGHTHPSVSQIVREMAAKGYITEKKGEQDGRKNFVLLSS
                     AGQQLREKMQAQLDDVTAAIEKAMKETHHDLWKAIGEWELLLDQKSLLRRVLEEKKER
                     ESRQVQIVDYIPAYQPAFRQLNEEWITKYFRMEEGDYKALDHPQEHILDKGGHIYIAL
                     YKDEPVGACALIPMADGGFELAKMAVSPKAQGLGIGYLIGQACIEKARALGAPRVYLE
                     SNTTLKPAINLYHKLGFRKAAGPPSPYERANIQMELVF"
     gene            4813..6318
                     /gene="glpK"
                     /locus_tag="VKQ52_04155"
     CDS             4813..6318
                     /gene="glpK"
                     /locus_tag="VKQ52_04155"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HLZ86407.1"
                     /translation="MSASYILSLDQGTTSSRAILFDRNGQIRQMAQKEFTQYYPQPGW
                     VEHDAEEIWSSQYGVLAEVLAKSNLTTREIAAIGITNQRETVVVWERASGRPIHPAIV
                     WQDRRTAGYCDELKARGMGKLIGERTGLVIDAYFSATKLKWILDHVPGARARADKGEL
                     AFGTVDSWLVWKLTAGKLHITDTTNASRTLLFNIHTGRWDEELLRLFGIPEAILPGVR
                     ASSEVYGHTGSIMAPGSIGMPIAGIAGDQQAALFGQLCTQPGMVKNTYGTGCFLLMNT
                     GTKAVTSRNQLITTIAWTRGGKTEYALEGSIFIAGAVVQWLRDGLHFIRNSQQVEAMA
                     AEAEDTGGVYCVPAFAGLGAPHWNQYARGSLFGMTRGTTQAHIARAALESIAYQTYDV
                     LKAMEADSGIVLKELRVDGGATANNLLMQFQSDILGVPVVRPVVTETTALGAAYLAGL
                     GVGYWSSEEDIRQQWAVDRRFQPVMKEERARQLLNGWQRAVKAAIAWADNT"
BASE COUNT         1218 a         1396 c         2274 g         1487 t
ORIGIN      
        1 aaaggagttg gggatgaaga gcagccagat gattgaaacc gcgatcgtaa ccaggcggcg
       61 tcgttgtggc ttttttgtga gccaggagga aagcgcatag ggtatatagg ctaaaaagag
      121 gttccagatc atcatgatga acagggtgct cccggtgtgg aggatgcgga tcgcgaccag
      181 caggcagctg aagaagatgc ttgcggtgag caggcggtcg atctcggtgc ggagaaagaa
      241 gtacctgcta aaacgggagg tttgaaatga aaaggttgtc atatgatctt tgggatgaag
      301 atgagcagac ctgcgatgac ggcgatggcg gcggctgtga ggacggcgcc cgcggcgagg
      361 tctttaatgt gttttatttc gggatgccgt tcttttgtga tgtgatcggc gagtttttcg
      421 atgcaggtgt taaggatctc cgcgacccag acgaggccgg tcacggcggc gaggatcgcg
      481 gcttcgggga gggagacctt cagcagccag ccggtgatga tgacggcgat ggtggcgacg
      541 aggtggatac gggcgttgtg ctcgtgccag acgaattgcc ggatgccgcg gatggcgtgg
      601 gtaaagctga ggagccggct gcggatggag aatttttcct gttgcatgaa tcttgttttt
      661 gctattgcca gtttcaggct atcgcgggga ggttaatggt tgtcgattgc cgcttcacct
      721 ttgataggat ggcttccagt aaaggtagca tcttgttaag accctgggtc tttatcataa
      781 aattccacac cggttcgaat ttcttccagc cgcctgcgta ggcgaagtgt tttatggcgt
      841 gtttcaggtg atcgtgtccg aggcttgctt tggcgatatt gctccaggaa tagaaggagc
      901 ggtaggcgaa gtcatagcct tttttcaatt cggcggcgga gaggccgatc gtcctgtaga
      961 ccacctgccg ggtatcgtac tgatcccagt tgcgatggag gatgcggccc tgggattcca
     1021 tttccctgaa gagccgggta ccgggatagg gggtgaggat atgaaaggtg caggtggtga
     1081 gggaattctt tacggcccag tcgactgttc ttttgaacac gtcggggtcg tcggcgtcga
     1141 ggccgaatac gaagctgccg ttgatcatga tcccgaggga gtggaggcgg cggatggcct
     1201 cttcgtaatc ctggccgatg ttttgttttt tattgccttg caggaggttg ttgcggctca
     1261 gggtctcgaa gccgacgaag acgctgcgca tgcctgcggc ggcggcggat tctatgagat
     1321 cgccgcggag gatggcgtcg atggtggagg cgccctggaa gacacggtcc atgcctttca
     1381 tgccgtcgaa gagggcggtg gcgaagcgct ggtgtcccag gagatggtca tcaaggaagt
     1441 agaggtgccg gcccgggagg cgatcgatct cggcgagtgc agcgtctacc tgttgggtat
     1501 agaaggagcg gccgccttcg aagaaggcgt ctttatagca aaaatcgcag tgatgggggc
     1561 aaccgcggga gacgacgatg gaattgggga cgaggtagcg gttgcgtttg ataagatcgc
     1621 ggcggatcgg cgggatggaa gtgagatccc gttcgcgatt ggaatagcgt ttttggggtt
     1681 tgttgttcct gaggtcgcgc aggaattggg ggaaggtgtc ttcgcctggt ccgataaaga
     1741 tgctatccgc gtgggcggcg gcttcatccg gcagggaggt gacatggagg ccgcccaggc
     1801 agacgtaggc gcctttttcc cggtagtggt cggcgatggc gtaggcgcgg aaggcgtttg
     1861 tgatatagac ctggatgatg acgaggcggg gttcgtcgtc gaggttgagc tcttcgacgt
     1921 gttcatcctg gagatcgatc tcgtcctccg gggagcagta agccgccagt gtggccagtc
     1981 ccagtggggg gaagagggaa tactttatcg gtcgccagaa ggggcttttg gcttcggtca
     2041 gtgcgggtaa gatcattttg atcttcatgg gaatggattt agtagcggat atgctggatg
     2101 agggtatagg ccaggagcac aacgagcagc aggatgctct ggatggcgat agcggtgttg
     2161 agccaatacc gccggtaggc tacgcccagc tccaggccac ggggaccgtg gtgcaggcgt
     2221 agctttatga tggcggcaac attaaagagt acagccagca acagggatcc gaggatgagc
     2281 tgggctttat ggagggcaaa gaggtcgaat ggcgattgga ggaaggaggg tgcgatatta
     2341 tagtacatgg tttttgctcc gaagcagatg cggaccagga gggtcagcag gaagtagcag
     2401 gccgggatca tgagggtgat gccgaggatg ccggaggcga gggggcgaag gaagggagag
     2461 cgtgtcatct ttttgttttt tgttttgttc gttgttgtgg gcggatttct aaaagtgctt
     2521 tgaatttcaa agttataggt caaaaaaaat taggctttgt tcacgccgcg gatcatgttc
     2581 tcgagggctt cgaggtgatc cttgaatgct ttttcacctg cttttgttat ggagtaggtg
     2641 gtatttgttt tcctgccgat aaagcctttg tgcactttta taaagccgtt ctcttcgagg
     2701 tttatcaggt gtgaggcgag gttaccatcg gtgagttcga gcagctgttt gagatcgttg
     2761 aagctgacct cttcgttcac caacaggatg ctcatcactc ccatacggat gcgactatcg
     2821 aatactttat ttagattccc gatcggattc ttcatgatct ttcgtatttc caccacatga
     2881 tcagcccgta aatgatgtgc aggacgccga agccggcggc ccagaagtac aacccttcgt
     2941 ggggatagta catgttgacg catccgagcg ctatttccaa aagtccaaga tatcgaatat
     3001 ccgttaaagt gtatttactg gcgttgacga gggcgaggcc gtagaatacg aggcaggagg
     3061 gtgcgacgta gttccagccg gagtggtcga gtagtccgag gacgaaaaag ccgccggctg
     3121 cgagggggat ggccatgttg acggcgagtt ttttcgaggc gtggtcccag atggggagtt
     3181 tgctgcgccg ggctttccgc caggtgaacc aggtggaggt agcgagggcg gcgatgagga
     3241 cgccgagggc gaggaggatc atgcgcagtt cgaaccaatc ccgggcttcc gcgggccagc
     3301 gttcgggggc cgcggtgtct acactggaga cataggatct tatccagccg cgggctatcc
     3361 aggcaccgat gagggcgcaa acgccggcgc tgaccccgct cagtccgctg agggagatga
     3421 agcgggagct gcgttccatc atgcgtttga tgtcctgcag ggtttcgagg gattgctcgg
     3481 ttggactttg gttcgtcata aaagcacttt gaaatacaaa gtaaagggag atttttggat
     3541 tgtgcaagtt ttgggcggat ttttttggga cgcccggggc gggtgttggt ttgggtgttg
     3601 ggtttgcctg gggatggatg ctgggttggc ccacgggttt gttgggcctg ggttgttggt
     3661 tggagggttg gcctgggggt taggtgctgg gcttgggtgg atgttgggcc tgggttgttg
     3721 ggtggaggcg ttgggcgggt gtagggttgg atggctttgg ggggcaggcc gggccgttag
     3781 aacacgagtt ccatttggat gttggcgcgt tcgtaggggg atggggggcc ggcggctttg
     3841 cggaagccga gtttgtgata gaggttgatg gcgggtttga gggtggtatt gctttcgagg
     3901 tagacgcggg gggcgccgag ggcgcgggcc ttttcgatgc aggcctggcc gatgaggtag
     3961 ccgatgccga ggccctgggc ttttggcgag acggccattt ttgcgagttc gaagccgccg
     4021 tcggccatgg ggatgagggc gcaggcgccg acgggttcgt ctttatagag ggcgatatag
     4081 atatgcccgc ctttgtcgag gatgtgttcc tgggggtggt cgagggcctt atagtcgcct
     4141 tcttccatcc ggaagtattt tgtgatccat tcctcgttca attgccggaa ggcgggttga
     4201 taagcgggga tgtagtcgac gatctgtacc tgccggcttt ccctttcctt tttttcttcc
     4261 agcacgcggc ggaggaggct tttttgatcg agcaggagtt cccattcgcc gatggctttc
     4321 cagagatcgt ggtgggtctc tttcatcgct ttttcgatgg ccgcggtcac gtcgtccagc
     4381 tgggcctgca tcttttcgcg gagctgctgg ccggcggatg agagcaggac aaagttcttg
     4441 cggccgtcct gttcgccttt cttttcggtg atatagcctt ttgccgccat ttcgcggacg
     4501 atctggctga cggaggggtg ggtatggccg atctcgcggg cgatgtcggt gatcgatcgc
     4561 gcttcggcag gcgagaggga gtagaaaacg gggaaccatt tcggctggag gtcgacctgg
     4621 tagagctggt agatagctgc ggcctgttcg gtcatttgtt cactgagccg gcggaggcgg
     4681 ctgccgaggg ccattttgcc cgtgcgatcg tagaaatcca tgactttggc attaattacg
     4741 taagtgctta cgtaaatgta ggcagaattt gctctccggc caaattcttt tattttccta
     4801 catttaaagt atatgtcagc atcttacatc ctttctttag accaggggac taccagctcg
     4861 agggccatat tatttgaccg gaacgggcag atccggcaga tggcgcaaaa ggagtttacg
     4921 cagtactacc cgcagccggg ttgggtggag catgatgccg aggagatctg gtcctcgcag
     4981 tatggggtgt tggcggaggt gctggccaag agcaacctga ccacgaggga gatcgcggcc
     5041 atcgggatca ccaaccagcg ggaaacggtg gtggtgtggg agcgggccag tgggcggccg
     5101 atccatccgg ccatcgtctg gcaggatcgc cggacggcgg ggtattgcga tgagctgaag
     5161 gcgcggggga tgggcaagct gatcggggag cggacggggc tggtgatcga tgcctatttt
     5221 tccgccacca agctgaagtg gatactggat catgttcccg gcgcgagggc ccgggccgac
     5281 aagggggagc tggcttttgg caccgtggac agctggctgg tctggaagct gacggccggg
     5341 aagctgcata tcaccgacac gaccaatgcg tcgcgcaccc ttcttttcaa tatccatacc
     5401 gggcgatggg atgaggagtt gttgcgtctt ttcgggatcc ccgaggcgat cctgcccggg
     5461 gtgcgggctt cgagcgaggt atatgggcat accggcagca tcatggcgcc gggctctatc
     5521 gggatgccga tcgcggggat cgcgggggac cagcaggcgg cccttttcgg gcagttgtgt
     5581 acgcagcccg ggatggtgaa gaatacctat ggtacgggct gttttctcct gatgaatacc
     5641 gggacgaagg cggtgacgtc ccggaatcaa cttatcacga cgatcgcgtg gacccgtggc
     5701 gggaagacgg agtatgccct ggaggggagc atttttatcg cgggggcggt ggtgcagtgg
     5761 ctgcgggacg ggttgcattt tatccgcaac tcccagcagg tggaggcgat ggcggccgag
     5821 gcggaggata cgggcggggt ctattgtgtg ccggcatttg cggggcttgg ggcgcctcac
     5881 tggaatcaat atgcgcgggg aagtcttttt gggatgacga gggggactac gcaggcgcat
     5941 attgcccggg ccgccctgga gagtatcgcg tatcaaacct atgatgtatt aaaagcgatg
     6001 gaggccgaca gtggcatcgt gttgaaggag ttgcgggtgg atggcggcgc cacggcgaat
     6061 aacctgctga tgcaattcca atccgatatc cttggggtcc ccgtggtgcg gcctgtcgta
     6121 acggagacga cggcgcttgg cgcggcctac ctggcggggc ttggcgtcgg ctactggtcc
     6181 tcggaagagg atatccggca gcagtgggct gtggaccggc ggttccaacc agtgatgaag
     6241 gaggagcgtg cgcggcaatt gctaaatggc tggcagcgtg ccgtgaaggc ggcgatcgcc
     6301 tgggccgaca atacctaatc atgaaaagac aggaaatgat cgcggccctg gaggaacggg
     6361 gcgcgcagca atggg
//
LOCUS       DATGFG010000226         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_876314, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000226 DATGFG010000000
VERSION     DATGFG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876314"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VKQ52_04160"
     CDS             <1..1293
                     /locus_tag="VKQ52_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ86408.1"
                     /translation="IYFSNAKDREDQYYKRLHQQAVTKADLLLGAKVQPSVLQLIYKN
                     SLNSLPQEEVAVYDTTFHLLYHDAVYIDRVKETRGMIDTIVRQHEIHFYVNDLQAIGF
                     LYPYNGKEYVITAAAKDIDGLAKLHDLRIALVVGFLIAVLLTLMAGTIFSRQALQPVS
                     QIVDKVEEITASRLDLRLGEGRGKDEIAELAITFNRMLDRLENSFEAQKQFVSNISHE
                     LRTPMAAIIGELEIAAKKGRTIPEYEQMNNLVLHDARRIVRLSNGLLDLARASYDQAE
                     IAFREMRLDELLLDSRQAILKSNPDYGINIIFEREIEDDDYISVKGNEYLLKVAFTNL
                     IENGCKFSEDHQSAIAISFHATNTILRFSDNGIGIAPEDIAHIFTPFYRGANRNYAEG
                     HGIGLPLTNKIILLHQGTLTVSSRVNEGAIFTVELPHL"
     gene            1636..1824
                     /locus_tag="VKQ52_04165"
     CDS             1636..1824
                     /locus_tag="VKQ52_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86409.1"
                     /translation="MKSIGTWTYRIIMLLLVVFLIDFDCTNMRRTVEKSNHMILTNLV
                     LGGLFLLLLISQINKKRL"
     gene            1826..>3112
                     /locus_tag="VKQ52_04170"
     CDS             1826..>3112
                     /locus_tag="VKQ52_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLZ86410.1"
                     /translation="MNTYIKEAGKKLLSLNRGNEKSGHDLDPAAATKLRTAARAGRNE
                     LFVLLDAGESGLGEIEVREKQYQFGANEVAHEKAPAWYIQLLQAFINPFIGILIVIAI
                     VSLITDVFLAAPADRDYKTLMVVSIMVMLSSFLRFWQEFRSNQAAEQLKSMVKTTAAV
                     LRRTVLGPYKQEIDIKDIVPGDIIYLSAGDMIPADCRIFQSKDLFVSQAMLTGEALPV
                     EKREHAIPDADTRSPLELENCCFMGTNVVSGTAMAAVVTTGGQTWFGSISRAVTGKRA
                     ETSFDKGVNSVSWLLIRFMLIMVPLVFLINGLTKGNWLEALLFAIAVAVGLTPEMLPM
                     IVTANLAKGAVNMSKRKVIVKRLNAIQNIGAMDILCTDKTGTLTIDKIVLERHLNVMG
                     VEDEEVLKWAYLNSYHQTGLKNLLDVAVLEHGELHDS"
BASE COUNT          775 a          927 c          756 g          654 t
ORIGIN      
        1 atctactttt ccaatgccaa agaccgcgaa gaccaatatt acaagcgcct tcaccagcag
       61 gccgttacca aggcagacct tcttttaggc gccaaagtcc agccctccgt tctgcaactt
      121 atctacaaaa actcgctgaa ctcccttccg caggaagaag tcgccgtcta cgacaccacc
      181 tttcatctac tctaccacga cgccgtatat atcgaccggg taaaagagac caggggtatg
      241 atcgatacca ttgtccggca acacgagatc catttctatg tgaatgatct ccaggctatc
      301 ggcttcctct atccctacaa cggcaaagaa tacgtcatca ccgcggcagc caaggacatc
      361 gacggcctgg ccaaactgca tgacctgcgc attgcgctgg ttgtcggctt cctcatcgct
      421 gtcctgctga ccctgatggc cggcaccatc ttctcccgcc aggccctcca gccagtgtcc
      481 caaatcgttg acaaggtgga agaaataacc gcatcccgtc tggacctgcg ccttggcgaa
      541 ggaaggggca aagacgagat cgccgaactg gccattacct tcaaccggat gctggacagg
      601 ttggagaact ccttcgaagc ccaaaaacaa ttcgtatcca acatttccca tgaactcagg
      661 acgcccatgg ccgccatcat cggcgaactg gaaattgccg cgaaaaaagg ccgcaccatc
      721 ccggaatacg aacaaatgaa caatctcgtc ctgcacgatg cccgacggat agtccggctc
      781 tccaatggcc tgctcgacct ggccagggcc agttacgacc aggcggagat cgccttcagg
      841 gaaatgcgcc ttgatgaact cctgctggat agccggcagg cgatcctaaa atccaacccg
      901 gactatggta tcaacatcat cttcgagcgg gagatagaag acgacgacta tatttcggtg
      961 aaagggaacg agtacctgtt aaaggtagct tttaccaacc tgatcgagaa cgggtgtaaa
     1021 ttctccgagg accaccaatc cgcgatcgcc atcagcttcc acgccaccaa tacgatcctg
     1081 cgcttctccg acaatggcat cggtatcgcc cccgaggata tcgcccatat ctttacccct
     1141 ttctacagag gcgccaacag gaactatgcc gaaggccatg gcatcggact gccgcttacc
     1201 aacaagatca tcctcctgca ccagggcacc ctcaccgtca gttcccgggt caacgaaggg
     1261 gccatcttta ccgtagaact gccgcattta taacgcaact ctatttgcag caatgctgca
     1321 tccatggcca ctctaatagc tttctaattt ttttctaata cctctctaac aggctttcca
     1381 ggaagagctt cctagctttg cggagcaaac aaaaaagtac cagcgtttat gaccagcgac
     1441 agtcattccc gtattcgaca tattatctaa tctctccctt ccaggacggc ttttcccagc
     1501 taccccggca ggggggtcaa caggagaagc atgctcagca ttccgcagtt ttccacgcgt
     1561 cattttcttg ctctatcccc gggcgctgcc atgggcctcg aggaagaaaa cgatctataa
     1621 accaaaacaa gacaaatgaa aagtatcggc acctggacat acagaataat aatgttgctg
     1681 ctcgtggtct ttttgatcga cttcgactgc accaatatgc ggcgcactgt cgaaaagagc
     1741 aatcatatga tcctcaccaa tcttgtcctt ggcggtctgt tcctcttgct actcatctcc
     1801 caaatcaata aaaagaggct gtaacatgaa cacctacatc aaagaagccg gaaaaaagtt
     1861 attgtccctc aaccggggga atgagaagag cggccacgac cttgacccgg ccgcggccac
     1921 aaagttgcgg accgctgccc gcgcgggcag gaacgagctg tttgtcctgc ttgatgcggg
     1981 cgagagcggg cttggcgaga tcgaggtacg ggaaaaacag taccagttcg gggccaacga
     2041 agtcgctcac gaaaaggctc ccgcctggta tatccagtta ttgcaggcgt ttatcaaccc
     2101 gtttatcggc atcctgatcg tcattgccat cgtttccctt atcacggacg tctttttggc
     2161 tgcaccggcc gaccgggatt ataagacgct gatggtggtt tcgatcatgg tcatgctcag
     2221 cagcttcctt cgcttctggc aggagttccg cagcaaccag gcagcggaac agctcaagag
     2281 catggtaaaa acgacggccg ccgtcctgcg caggacggtg ttgggcccct ataaacagga
     2341 gatcgatatc aaggacatcg ttcccggcga cattatctat ctctccgccg gtgacatgat
     2401 cccggcagac tgccgcatct tccaatccaa ggacctcttt gtcagccagg ccatgctcac
     2461 cggcgaagca ctacccgtcg aaaaaaggga acatgccata ccggatgcag atacccgcag
     2521 ccccctggag ctcgaaaact gctgcttcat gggcaccaat gtcgtcagcg gcactgccat
     2581 ggcagcggtg gtgaccacgg gtggccagac ctggttcggc tctatcagca gggccgtcac
     2641 cggcaaacgg gccgagacca gtttcgacaa gggggtcaac agcgtgagct ggctccttat
     2701 ccgtttcatg ctcatcatgg tgcccctggt cttcctgatc aacggcctga ccaagggcaa
     2761 ctggctcgag gccctgctct tcgccatcgc cgtcgccgtc gggcttaccc cggagatgct
     2821 tcccatgatc gtcaccgcca atcttgccaa gggcgccgta aacatgagca agagaaaggt
     2881 gatcgtcaag cggctgaacg ctatacagaa catcggcgcc atggatatcc tctgcaccga
     2941 caagaccggt acgctcacca tcgacaagat cgtcctcgaa agacatttga atgtcatggg
     3001 ggtcgaggat gaggaagtat tgaaatgggc ttatctcaac agctatcatc aaaccgggct
     3061 gaagaacctg ctcgatgtag cggtcctcga acatggggaa ctgcacgatt cc
//
LOCUS       DATGFG010000227         8199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1446962, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000227 DATGFG010000000
VERSION     DATGFG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446962"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..459)
                     /locus_tag="VKQ52_04175"
     CDS             complement(115..459)
                     /locus_tag="VKQ52_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86411.1"
                     /translation="MIRLFLISALLGGLAFRGHAQPLTEWIGQLAALQTLQQTVEQGY
                     ATVAGGLQTIGDLRYGEYRLHAGWFGSLDTVKPVIDEDPRVQALRSRLATMITQLRTA
                     LDYWQRQSILSP"
     gene            complement(470..3604)
                     /locus_tag="VKQ52_04180"
     CDS             complement(470..3604)
                     /locus_tag="VKQ52_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032991 - protein-containing complex
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraG family conjugative transposon ATPase"
                     /protein_id="HLZ86412.1"
                     /translation="MASIKLQQILPHAEVTRNTVISRNGEESVILEVMKPPAFTLFET
                     EIAILNGALAKGIACFPDKTTFHLQEWYTDAAYEPPFRDNGHPLDKASDQHFKGRRHQ
                     HHRCFLIITRRLEGERPATSALSSLIRPTLVPGSVLDPTKRETFFACCKQLTGLLEST
                     GLVKLRQLENDELAGTTESAGLIEQYMTLNPDHRQPQLRDVDITNERIRVGHKQVILF
                     TLADAANLPPTCEPSRRYEPYSTTVTELPVGFASHLGPLLPVDHVYNLFIVKDDPRRE
                     LKKLETQRRRLKSLSGTSRENAVAAADIEAYLDQAAQPGQIPVRVHINVMAWTDDPTA
                     LTPVTNEVSSAISRTGATPHLETVGAPMIWCAGIPGNAGNLPINETFLSFLTTALCWF
                     IPESSGPDVTMLRGLRLGDRLTGIPINIDLSDEPMRKGLVTNRNKFILGGSGSGKSFF
                     TNHLVRSYHAQGAHIVLLDIGGSYKSLCRLLDGYYFECTEKEPIRFNTFLLASGETLD
                     TEKKESLKALLLTLWKKADEGFTRSEYVTLSRMLEGYFEWQTKNPGTFRCFDSFYEWV
                     RDEFLPELAEEGMREEDFDGKNLLYVLSPYYKGGEYQYLLNARENTDLFHQRLIVFDL
                     DSIKDHPILFPVTTLIVMELFISKLRKLKGVRKVILLEEAWKAIAKEGMSEYIKYLFK
                     TVRKFYGEAIVVTQDIEDIVSSPVVKNSIINNADCKILLDQSKFLNRFDQIQDLLGLT
                     DNDKTLVLSLNKANDPDLKYKEVFIGLGPNHRRVYRVEVSLEEYLAFTTEETERVKVM
                     DYAEKKGGIREGIAALAADIRSGAVKWMVALFCTAAFLLAPQGKASAQLLDIIDEAIK
                     EALETADLKLQQLQTQTLWLQNTQKALENSMAGDLLDDITGWVQQQDALYSEYYNELW
                     QVKTVFTDYGRTKGLVDRQVQLVKEYQLATAAVRSDPHFSAAELQHILSVYSGILDAS
                     IRNAGRLATVITAFATQMDDAGRLRIIDETAAETDRNYTALRSYAQENSLLSLQRARD
                     AADLQTIKTLYGLP"
     gene            complement(3607..3930)
                     /locus_tag="VKQ52_04185"
     CDS             complement(3607..3930)
                     /locus_tag="VKQ52_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4133 domain-containing protein"
                     /protein_id="HLZ86413.1"
                     /translation="MTTSVYPTYRAIGRPIEFQGLQGPYILAGAMALIADLLLFVLLY
                     CCGTPPWLCIGIAFGLGTAAVVIARALSRRFGVHGWMKHLAAKRLPDAIRFNSRQVYL
                     NLIKK"
     gene            complement(3973..4383)
                     /locus_tag="VKQ52_04190"
     CDS             complement(3973..4383)
                     /locus_tag="VKQ52_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4134 domain-containing protein"
                     /protein_id="HLZ86414.1"
                     /translation="MLFFISWGRFLTVILLSTAAYYAILLLLFHRNKIFDWLKKRTPL
                     LVAGTAGALAAHAQDGNQGINQANTLIRSYYDTGVQLMYAIGAVLALVGAVRTYREWN
                     AGHQQEAYRAAAGWFGSCVFLVVVATVIKSFFGL"
     gene            complement(4499..5053)
                     /locus_tag="VKQ52_04195"
     CDS             complement(4499..5053)
                     /locus_tag="VKQ52_04195"
                     /inference="COORDINATES: protein motif:HMM:NF020914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RteC domain-containing protein"
                     /protein_id="HLZ86415.1"
                     /translation="MDVQVVERMRELEQAILNNETEPDRLKRVARYIELAIRAIEPIM
                     EAARNDPEMREKTDGATYLLVYLQYALLSQQGKNGQSVVPKDEPSIPLVEYRGKAINL
                     IELASALLLTKDFYVDGRPMTRAFIAALIERIFGIPADKFDGKVQDMKKRKIGSAVFL
                     QTLANSLSAYLGRLPDDRPRRKGI"
     gene            complement(5252..5830)
                     /locus_tag="VKQ52_04200"
     CDS             complement(5252..5830)
                     /locus_tag="VKQ52_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLZ86416.1"
                     /translation="MEELLQFLCSTHRLSPECIAQLHKIVKFQKVPKGHAILKIGEVN
                     RHLYFIRKGLLHCHYLVNDSSVSAWFFMENATVVSIGSFYNQSKSEDCIEPVEDTELF
                     YITREEFDHLCRTYHDFCYLANLLFIKYLKEFQEHSRLIRKLKSKDRYDTAVLKYPDL
                     FQRVPAKLLASWLSMEKETLSRGRSRNRGRKK"
     gene            6140..6523
                     /locus_tag="VKQ52_04205"
     CDS             6140..6523
                     /locus_tag="VKQ52_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ86417.1"
                     /translation="MQRKKKIAGYDHPIARTKLYDVPAIELAKSIIEKDPVAHIHIPM
                     LADKAGINEFKLKVGFRELFQTSPYQYRLHLCLEKARLLLEETDDTIDQIASKVGFDT
                     YNGFSTAFKKAYSIAPTEYRNRQSA"
     gene            complement(6525..>8199)
                     /locus_tag="VKQ52_04210"
     CDS             complement(6525..>8199)
                     /locus_tag="VKQ52_04210"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ86418.1"
                     /translation="TTMQTLQFQPGGVFEQQQQSQSINKQDQHSVLGLYERTVGSRSA
                     FQFSFHSNFFQNQNQFSSTSSSIFRDTLENASLRAIRDQTSRQSIVGDVSWRIQSGKQ
                     LGRIFSIKTGFLTTDYSTDGYLYTINSFFKPDGNALGADTVDQRKHITDHSLKVHGDI
                     GYTQPFFTGSTLSFILGSPVEYDNPLQATFGRGQGKYDEAIDSLTSYIGTRTINSQGM
                     IVLQGKSRHINYTFITTWLDYIYRQRDQQTDSVFHLHYISWVPRFLLNYTPRASFNVK
                     IDYSAATQQPSISQLAPIKNNNDPLHLTLGNPNLRPGTDQKLELGFHWLRSWIVAVNL
                     NFRVSNNSISTKTTTDSLGRQISQPVNVNGGSSAGANFSVNHRLAGIDWSLHTADSYN
                     RTINFLNTDLNRNDLYSISEGIGASRYVERKYSFQFNTNFSWLYSRSSVNISTPLHYW
                     TQSHFGMVSLFFVPHYEIGTNATFTWQQKTNAFSNSSSVLLWNAYVSRNFLRDKLAVK
                     ATINNLLDQNAGITRTSISNISTETSTNILGRYWMVSVMYHFDKKFSSK"
BASE COUNT         1848 a         2084 c         2221 g         2046 t
ORIGIN      
        1 ttctgggcat cgaaggccag ttgctcggtg agatcgagga tggattgcgc ccggcctgtc
       61 accgacagga gcccgagcac aaggacaaaa agcatttttt gcataagaat ggttttatgg
      121 tgatagaata gattgccgtt gccaatagtc gagcgccgtc cgcaattgcg tgatcatcgt
      181 cgccagccgg ctgcggagcg cctgcacccg cggatcttcg tcgatgacgg gcttgacggt
      241 atcgaggctg ccgaaccagc cggcatgaag ccggtactcg ccgtagcgga gatccccgat
      301 ggtctggaga ccgccagcca cggtcgcgta cccttgctca accgtttgct gaagcgtttg
      361 cagggccgct aattgcccga tccattccgt gaggggttgt gcgtgccccc ggaaggcaag
      421 gccgcccaaa agggcgctaa tgagaaatag acgtatcatg ggtttggttt tatgggaggc
      481 cgtagagggt tttgatggtt tggagatccg ctgcgtcccg ggcgcgttgg aggctgagca
      541 gactgttctc ctgggcatag gatcgcagcg cggtgtagtt gcggtcggtc tcggcagctg
      601 tctcgtcgat gatgcgcaat cgccccgcgt cgtccatctg ggtggcgaat gccgttatca
      661 cggtggcaag gcggccagcg ttgcggatac tcgcgtcgag gatgccgctg tagacgctta
      721 ggatatgctg gagctccgca gcgctgaaat gcggatcgct tcgcacggca gccgtcgcta
      781 actggtattc tttcaccagt tgcacctggc ggtcgacaag ccccttcgtc cggccatagt
      841 ccgtaaaaac ggtcttcacc tgccacagct cattataata ctcgctgtag agggcatcct
      901 gctgttgcac ccagccggtg atatcgtcga gcagatcgcc cgccatactg ttctccaggg
      961 ccttttgagt attctgtagc cagagggtct gggtttgcag ttgctggagc ttcagatcgg
     1021 ccgtttcgag cgcctccttt atcgcctcgt cgatgatgtc cagcagttgc gccgaagcct
     1081 tcccctgcgg cgccagcagg aatgccgccg tgcagaataa cgctaccatc cacttcaccg
     1141 ctcccgaccg gatatccgcc gccagcgcgg ctatcccttc ccggatgccg ccctttttct
     1201 ctgcatagtc catgaccttc acccgctccg tctcctccgt cgtaaaggcc aggtattctt
     1261 ccagcgagac ctcgacccgg tagacccggc gatggtttgg gcccagcccg atgaatactt
     1321 ccttgtattt gaggtcggga tcgtttgcct tgttcagcga caggaccagg gtcttgtcgt
     1381 tgtcggtgag cccgagcagg tcctggattt ggtcaaaccg gttgaggaat ttactctggt
     1441 ccagcaggat cttgcagtcg gcgttgttga tgatgctgtt cttcaccacc gggctcgata
     1501 cgatgtcttc gatatcctgg gtgacgacga tggcctcgcc atagaatttg cggacggtct
     1561 tgaacaggta tttaatgtac tcactcattc cctccttggc gatggccttc caggcctctt
     1621 ccagcaggat caccttgcga acgcctttca gcttccgcag cttgctgata aagagctcca
     1681 tgacgatgag ggtggtcacc gggaaaagaa tgggatggtc cttgatggaa tcgaggtcga
     1741 agacgatcag ccgttggtgg aagaggtcgg tattctcccg ggcgttcaac aggtactggt
     1801 attcgccgcc tttgtaatag gggctgagca catagagcag gttcttgcca tcgaaatctt
     1861 cttcccgcat accttcttca gccaattccg gcaggaattc atcccgcacc cattcgtaga
     1921 agctgtcgaa gcagcgaaag gttcccgggt tcttcgtctg ccattcgaaa tagccttcga
     1981 gcatgcggga gagggtgacg tattcgctgc gggtgaaccc ctcgtcggcc tttttccaga
     2041 gggtgaggag cagggccttc aggctttcct tcttttccgt gtcgagggtc tcgccgctgg
     2101 ccagcagaaa ggtgttgaag cggatcggct ctttctcggt gcactcgaag tagtagccat
     2161 cgagcaggcg gcacagcgat ttatagctgc cgccaatgtc gagcaaaacg atatgggcgc
     2221 cctgtgcatg atagctgcgt accaggtggt tggtgaaaaa ggactttccg cttccgctcc
     2281 cgccgaggat gaacttgttg cggttggtga ccagtccctt ccgcatgggt tcgtcggaga
     2341 ggtcgatgtt tatcgggatg cccgtaagcc ggtcgcccag ccggagaccc ctgagcatcg
     2401 ttacgtccgg gccgctggat tcggggatga accagcaaag cgccgtggtg aggaagctga
     2461 gaaaggtctc gtttatcggc aggttgccgg cgtttccggg gatcccggca caccagatca
     2521 tcggtgcgcc caccgtttcg agatgggggg tggcgccggt gcggctgata gcgctgctca
     2581 cctcgttggt gaccggggtc agggcggtgg gatcatccgt ccaggccatg acgttgatat
     2641 gcacccgcac cgggatctgt cccggctggg ccgcctggtc caggtaagcc tcgatatccg
     2701 ccgcggcgac ggcgttctcc ctgctggtgc cgctcaggct cttgagccgg cggcgctggg
     2761 tctccagctt tttgagttcc cgcctgggat catctttgac gataaagaga ttatagacgt
     2821 ggtccacggg cagcagcggc cccaggtggg aggcgaaacc gaccgggagt tcggtcaccg
     2881 tggtgctata gggttcgtag cggcggctcg gctcgcatgt cggcggcagg ttggcggcgt
     2941 cggcgagggt aaacaggatc acctgtttgt ggccgacccg gatcctttca ttggtgatgt
     3001 cgacatccct gagttgaggt tgccggtgat cgggattaag cgtcatatat tgctcgataa
     3061 ggccggcgct ttcggtggta ccggctaact cgtcgttttc cagctgccgc agttttacga
     3121 ggccggtaga ttccagcaga ccggtgagtt gcttgcagca ggcaaaaaat gtctcgcgtt
     3181 tggttggatc gagtaccgag cccggcacca gggtaggccg gatcagcgag gacagcgccg
     3241 aagtcgccgg gcgttcgcct tccaatcttc gggtgatgat cagaaagcag cgatggtgtt
     3301 ggtgtcgccg tcccttgaaa tgttggtcgg aggctttatc cagcgggtgg ccgttgtcgc
     3361 gaaaaggggg ttcataggcg gcgtcggtgt accattcctg taggtggaag gtagttttgt
     3421 cgggaaagca agcgatgcct ttcgcgagcg ccccattcaa tatggcgatc tccgtttcga
     3481 agagggtaaa ggcagggggt ttcatcactt cgaggatgac cgactcttcg ccgttgcggc
     3541 tgatgacagt gttcctggtc acctccgcgt ggggaaggat ttgctgtaat ttaatagaag
     3601 ccatggttat ttttttataa ggtttaaata gacttgccgg ctgttgaagc ggatcgcgtc
     3661 aggcaaccgc tttgcggcaa gatgtttcat ccatccatgg acgccgaatc gccggctcag
     3721 ggctctcgca atgactactg ccgccgtgcc cagtccgaag gcgatgccga tgcaaagcca
     3781 ggggggcgtt ccgcagcaat agagaagaac gaagaggagg agatcggcga taagcgccat
     3841 ggcgccggcc aggatatagg gtccctggag accctggaat tcgatcgggc ggccgatggc
     3901 ccggtaggtg ggatagacac tcgttgtcat atcacaagtt tttaaatccg gaaaatattt
     3961 ttttgaaagc tcttacaacc cgaagaaaga tttaatcacg gtggctacga ccacaagaaa
     4021 tacacagctc ccgaaccagc cggcggcggc gcggtatgct tcctgctggt gtcctgcatt
     4081 ccactcccta taggtgcgca ccgcgcccac cagggccagt acggcgccga tggcatacat
     4141 caattgaaca cccgtgtcgt agtagctgcg aataagggtg ttagcctgat tgatgccctg
     4201 attgccgtct tgggcatggg cagcgagagc gccggccgta cccgctacga gcagcggcgt
     4261 tcgctttttg agccagtcaa agattttgtt ccggtgaaaa agaagtagta agattgcgta
     4321 gtaggcggcc gtcgacagga gaatcactgt caggaaccgg ccccaggaaa tgaaaaacaa
     4381 catttgcatc gattttttgt gaaaaaattt gttacgatgc aaatgttgtt agaaagtgag
     4441 gcggcggcac acaagctggg acctacctta ggtgagatcg tttgctattg cctggcaatc
     4501 agatcccttt cctccgaggc cgatcgtccg ggagtctgcc gagataggca gataatgaat
     4561 ttgccaacgt ctgcaaaaaa acggccgaac cgatcttcct cttcttcatg tcctgcactt
     4621 ttccatcaaa cttgtcggcg ggtatcccaa aaatcctttc gatcagcgcg gctataaacg
     4681 cccgtgtcat aggccggccg tcgacataaa agtctttggt taacaacagg gccgaagcaa
     4741 gttcgatcag gttgatagcc tttccccgat actcaaccag aggaatggat ggttcatcct
     4801 tcggcacgac ggattgtcca ttcttgccct gctgcgataa cagagcgtat tgaaggtaga
     4861 ccaacagata ggtagcccca tctgtcttct cccgcatctc cgggtcattc ctggccgctt
     4921 ccattattgg ttcgatggcc cgaatggcca gctcgatata gcgggctacc cgctttagcc
     4981 ggtcgggctc cgtttcattg tttaggatgg cttgctccag ctcacgcatc ctttctacca
     5041 cttgtacatc catgactatt gattaaaaat taagaaataa cgacatatcc gttcctccaa
     5101 aaaacaaagg gcaccggcct tgcgcagccg gtacccagta ccaagttttg ccaatgccat
     5161 tctctccaca ttttcaccta cctcactgta ttggttactt gcagaacaaa aataagcaat
     5221 ggaaaattgc gtcagattga caaaaatcaa cttatttttt ccttccccga tttcggctac
     5281 gcccccggct tagggtctcc ttctccatgc tcagccagga ggccagcagc ttcgccggaa
     5341 cccgttggaa caggtccgga tacttcaaaa ccgccgtgtc atacctgtct ttggacttga
     5401 gtttccggat cagcctggag tgctcctgaa attccttcag atattttata aaaaggaggt
     5461 tggcaagata gcagaaatcg tgataggtcc tgcacaggtg gtcaaactct tccctggtga
     5521 tgtaaaacag ctcggtgtct tccactggtt cgatacagtc ctcgctcttc gactggttgt
     5581 agaaactgcc gatcgatacg accgtagcgt tttccataaa aaaccaggcg gacaccgaac
     5641 tgtcgttcac taaataatgg caatgtaaca accccttgcg gataaaatac aggtggcggt
     5701 tgacttctcc gatcttcaga atagcgtggc ccttaggaac cttttgaaat ttgacgatct
     5761 tgtgcagttg agcaatacac tcaggggaca accgatgggt ggaacaaagg aattgcagta
     5821 gttcttccat agagtaagat tagttggtga ataacatcta atttagtgaa cggtcgtcaa
     5881 actccgcaaa gcctgcacca tttttgcagt ctgcaacggc aaccattcca cgcggattat
     5941 ttttcggcta attgttgagg ttttcttcgt ccttaactga atcctcattt cattttcgtc
     6001 ctttagtaaa cctttttctg cttttggtat cctacgaata tttgctacgc gtcctgtaag
     6061 gttttaaatt agtcttccgg cactgccgca aagcccatta gtagcgacaa aaaccgttac
     6121 acattaaacc ttatcgatta tgcagcgtaa aaagaaaatc gcgggctacg accatcctat
     6181 tgcccgtaca aaactctacg acgtccccgc tattgaactc gcgaaatcca tcatcgaaaa
     6241 agatccggta gcccacatcc acattcccat gctggccgac aaggcaggga tcaatgaatt
     6301 caagctaaag gtcggcttcc gtgaactctt ccagacaagt ccgtatcagt atcgattaca
     6361 tttatgcctg gagaaggcga ggctcctcct ggaagaaaca gatgacacta tcgatcagat
     6421 agcatcgaaa gtaggcttcg acacctacaa cgggttcagc acagctttca agaaggcgta
     6481 tagcattgct cccaccgaat accggaaccg gcaatctgct taggctattt tgagctaaat
     6541 ttcttgtcga aatgatacat aacgctaacc atccaatacc gcccgaggat atttgtggat
     6601 gtttcagtgc ttatgttgct aatgctggta cgggtaatac cggcgttttg atcaaggaga
     6661 ttgttaattg tcgctttcac cgctagtttg tcccggagaa agttccggct tacgtaggca
     6721 ttccaaagaa gaacggaact gctgttggaa aaggcgtttg tcttttgctg ccaggtaaag
     6781 gttgcgttgg tcccgatttc atagtgagga acaaagaaaa gggataccat tccgaaatgg
     6841 gattgcgtcc aataatgtag gggggtggaa atattgacag aacttctcga atagagccag
     6901 ctaaaatttg tgttgaattg aaaactatac tttcgttcca catacctgct tgcaccgatt
     6961 ccctcgctaa tcgagtagag atcgtttcgg ttgaggtcgg tatttaagaa atttatggtc
     7021 ctgttgtagc tatcggccgt gtgtaaactc cagtctatgc ccgcaagtct gtggtttacg
     7081 gagaaattgg caccagcgga acttccacca ttcacattca cgggttggga gatttgacgg
     7141 ccaagactgt cagtagtcgt tttggtgcta atagaattat tgctaaccct gaaatttaaa
     7201 tttacagcta cgatccagga ccttagccaa tgaaacccca actccagttt ttgatcagta
     7261 cctggacgaa gattggggtt acccagcgtc aaatgtaaag gatcattgtt attctttatc
     7321 ggagccaatt gtgatataga aggctgctgt gttgcggcac tatagtcaat ctttacattg
     7381 aaagaagccc ttggcgtata attcagcaaa aatcggggta cccatgaaat ataatgcaaa
     7441 tgaaaaacag agtccgtttg ctggtcccgc tgacgataga tataatccaa ccaggtcgtg
     7501 ataaaagtgt aattgatatg gcgcgatttc ccttgaagga ctatcatccc ctgactattg
     7561 atcgtccggg tcccgatgta actggtaaga ctatctatcg cctcatcgta cttgccctgc
     7621 ccccggccga aggtcgcttg caggggattg tcatattcga cgggcgatcc gaggataaag
     7681 gagagggtgc ttcctgtaaa aaacggttgt gtataaccta tatcaccatg gactttcagg
     7741 gaatgatcgg taatatgctt tcgctggtcg accgtatccg cgccaagcgc atttccatcc
     7801 ggcttaaaaa aactattaat tgtatataaa tatccatcag tggaataatc ggtggttaag
     7861 aatccggttt tgatcgaaaa aatccggccc aattgcttac cggattggat acgccacgag
     7921 acatctccaa ctatgctctg cctactcgtc tgatcccgga tagcacgtaa gcttgcattc
     7981 tcaagggtgt ctctgaaaat actgctgctc gtagagctga attgattctg attttggaag
     8041 aaattactgt gaaagctgaa ctggaatgca gacctcgaac cgacggttct ctcgtacaat
     8101 ccgaggacag agtgttgatc ttgcttgttt atagattggc tttgctgttg ctgctcaaag
     8161 acgcccccag gctggaattg caaggtttgc attgtagta
//
LOCUS       DATGFG010000228         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1331867, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000228 DATGFG010000000
VERSION     DATGFG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331867"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VKQ52_04215"
     CDS             <1..835
                     /locus_tag="VKQ52_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86419.1"
                     /translation="FPNEVYPLVSRFNYVIAQVTIDSSQYYLDASEPWLGFGRLPERC
                     YNGYARVLDKENPSAVSLDADLLKESKMTLVILTNEDKGGLIGRLQSTPGFNESCAIR
                     QKVKKSGEQEFMKGIKTAYTADVDVSSLEIDSLQRPEEPLGVAYDLKITPDATQDIFY
                     FNPMLGEGYKENPFKAAERKYPVEMSHAMDETYTLTMDIPAGYVVDELPKSTKVLFND
                     DQGFFEYLIVKGDENIQFRSRVKLNQANFKPEDYATLRDFFGFIVKKESEQIVFKKKK
                     A"
     gene            832..2730
                     /locus_tag="VKQ52_04220"
     CDS             832..2730
                     /locus_tag="VKQ52_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HLZ86420.1"
                     /translation="MKRIVYMLLLAVPCLARAQGYSTALIPDSLRKGARAVVRESEFI
                     LEIKSPGKAVEKEHNVYTILNSNGDDIGGYTSWYGKFNSINEISGVLYDSLGKQVRKI
                     KRKDMQDRSYVDDMSLASDSRYLEYNFYCQVYPYTVDYQEEDEMNGILDFNDWQPLLK
                     SGISTQHSKYTIIAPKDYTIRYMLMNGAIPPVITENGDRKIYTWEAKNLPARHTEPSG
                     PRWRELVPYVLLAPSDFEAQGYKGNMSTWENYGKFIVQLRAGRDVLPDNIRRQVHALV
                     DTITDTRRKVYALYHYLQQNTHYMNVSLGIGGWQPFPPEYVATKKYGDCKALSNYMVA
                     LLKEAGIPAKYVEIRAGADEAPMIEAFPSFQSNHIITCVPMGKDSIWLECTNQTVPAG
                     YMGTFTGGRKAILIDDDGAHIVRTPSYSADDNTQCRVIRAQISAEGNLDASVNTMYRC
                     IRQDLPQAMMEEMSAEMRDKYLNSLFSLPTYTVDKSHYEAEKGALPVVKEDLHVLASN
                     YAGVSGRRLFIAPNLFDRSRKRLPADSSRKYDYITDKAYTDIDSVEIAVPPGYQPEAM
                     PKEVVIDGKFGRYKASVTFGNDKLIYYRYLQQSAKRYPPADYAGLVHFYEQLYQADNQ
                     RVVLVKKE"
     gene            complement(2745..3758)
                     /locus_tag="VKQ52_04225"
     CDS             complement(2745..3758)
                     /locus_tag="VKQ52_04225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLZ86421.1"
                     /translation="MFTLFLLFCLHSFAQPNFDAVDQLLKQNQKAFGGGYVMLVWKDG
                     KVIYQKQASQDFTAKSQAPIYGAGNWMTAALVMTFVDEGKLSLDDKVTQYIPLFGKYM
                     KGYITIRNCLTNTTGIKTDQSVTSVLTKQKFESLEDMVNSYANKHDIATNPSTEFFYS
                     NLGPNIAARVLEIVSKKSFDRLMRERITGPLKMHGTSFTNEEGGALNPSGGARSTAND
                     YINFLTMLLNKGTFEGKRILSEKAVEELETSQFADLPIRYLPKELTGAHFGLGEFITG
                     TNASGASAILACPNLLGTAPFIDKCRNYAAILIAEKPAEEKKPLYQNLVNLVGEAIGG
                     GCQ"
BASE COUNT         1006 a         1079 c         1083 g          885 t
ORIGIN      
        1 cttccccaac gaggtctatc ccctggtgag ccgcttcaat tatgtgatcg cccaggtgac
       61 catcgattca tctcaatact atctcgacgc cagcgaaccc tggctgggat tcgggaggct
      121 gcccgaaaga tgctataacg ggtatgcacg ggtgctcgac aaggagaacc cttccgctgt
      181 atcgctcgat gccgatctgt tgaaagaaag taagatgacc ctcgtcatcc tcacgaacga
      241 agacaagggc ggactgattg gacgcctgca gagcactccc ggctttaacg agtcctgtgc
      301 tatccgccag aaggtgaaga agtccggaga gcaggaattt atgaaaggga tcaaaacagc
      361 ctatacggca gatgtcgacg tcagcagcct ggagatcgat tccctgcaac gtccggaaga
      421 gccgttgggt gtcgcttatg acctgaagat cactcccgac gctacccagg atatttttta
      481 ttttaacccg atgctgggcg aaggatataa ggaaaaccct tttaaggcgg ccgagcgcaa
      541 gtatcccgtc gagatgtccc atgccatgga cgagacctat accctgacga tggacatccc
      601 cgcggggtat gtggtcgatg aactccctaa atcgaccaag gtgttgttca acgacgatca
      661 gggctttttc gaatacctga tcgtaaaagg agacgagaat atccagttcc ggagccgggt
      721 caagctcaac caggcgaatt tcaaacccga ggattatgcc accctgcgcg acttctttgg
      781 ttttatcgta aagaaagaaa gcgagcagat cgtttttaaa aagaaaaaag catgaagaga
      841 atagtgtata tgctgctgct ggccgtcccc tgtcttgcgc gggcgcaagg ctacagcacg
      901 gcgctgatac ccgattcgct ccggaaaggc gcccgggcgg ttgtccggga atcggagttc
      961 atactggaga tcaaatcgcc gggcaaggcg gtggaaaagg agcataatgt ctacaccatc
     1021 cttaactcca acggagacga cataggcggg tatacttcct ggtacggtaa attcaactcc
     1081 atcaacgaga tctccggggt tctctacgac agcctcggga aacaggtgcg gaagataaaa
     1141 cgcaaggaca tgcaggacag aagctatgtc gatgatatga gcctggcttc cgactcccgc
     1201 tatctcgagt ataatttcta ttgccaggtg tatccttata ccgtggatta ccaggaagag
     1261 gatgagatga acggcattct cgatttcaac gactggcagc ccctcctcaa atccggtatt
     1321 tccacccagc acagcaagta taccatcatc gccccaaaag actataccat ccgctatatg
     1381 ctgatgaacg gcgcgattcc gccggtgatc acggaaaatg gcgacaggaa gatctatacc
     1441 tgggaggcaa aaaacctgcc ggcgcggcat accgaacctt cgggaccccg ctggcgggag
     1501 ctggtgccat acgtcctgct ggcaccctcc gatttcgagg cccagggata caagggcaat
     1561 atgtccacct gggagaacta tggcaaattc attgtccagc tgcgcgcggg gcgcgatgtc
     1621 ctgccggaca atatccgccg ccaggtacat gcgctggtgg acacgatcac ggatacgcgg
     1681 aggaaggtat atgccctgta tcattacctg cagcagaaca cccattatat gaatgtctca
     1741 ttgggtatcg gcggctggca gccctttccg ccggagtatg tggccacaaa gaaatatggg
     1801 gattgcaagg ccctgtccaa ctatatggta gcgctgctga aggaagcagg catcccggca
     1861 aaatatgtcg agatcagggc cggcgcggac gaagcgccga tgatcgaagc cttccccagc
     1921 tttcagtcca accatatcat cacctgtgtg cccatgggaa aagacagcat ctggctggaa
     1981 tgcaccaacc agacggtgcc tgccggatac atgggaacct tcaccggcgg ccggaaagcg
     2041 atcctgatcg atgatgacgg tgcgcatatc gttcggacac cctcctattc tgcggacgac
     2101 aatacgcaat gccgggtcat ccgggcgcag atcagcgccg aaggcaacct ggacgccagc
     2161 gtgaacacga tgtaccgctg tatccggcag gacctgccgc aggcgatgat ggaagagatg
     2221 tccgccgaaa tgcgggataa ataccttaac agcctgttct ccctccctac ctataccgtg
     2281 gataagagtc actatgaggc ggaaaagggg gcattgcccg ttgtaaagga agacctgcat
     2341 gtcctagcct ccaactatgc cggggtgtcc ggccggaggc tctttatcgc gcctaatctc
     2401 ttcgaccgca gccggaaacg acttcctgcc gacagcagcc gcaaatacga ttatatcacc
     2461 gacaaggcct acaccgatat cgacagcgtc gagatagcgg taccacccgg ctaccagccg
     2521 gaggcaatgc caaaggaagt ggtcatcgac gggaagtttg gccgttataa ggccagcgtg
     2581 acattcggga acgacaaatt gatctactac cggtatctcc agcaatcggc caaacgatat
     2641 ccgcccgccg actatgccgg actggtccat ttctacgaac aactatacca ggccgataat
     2701 caacgggtcg tattggtaaa gaaagagtag gaggccagac cgcgttactg gcagccgccg
     2761 ccgatcgctt cgccgaccag gttcaccagg ttctggtaaa gcggcttctt ttcctccgcg
     2821 ggtttctccg cgataaggat cgcggcataa ttccggcatt tatcaataaa gggggccgtg
     2881 ccgagcaggt tggggcaggc caggatggcg cttgcgccgg aagcattcgt gccggtgatg
     2941 aattcaccca gtccgaaatg cgccccggtc agttcctttg gcaggtagcg tatcggcaga
     3001 tcggcaaact gggaggtctc cagctcttcc accgcttttt cgctcaggat gcgtttcccc
     3061 tcgaaggttc ctttattcag gagcatcgtc aggaaattga tatagtcatt ggcggtagac
     3121 ctcgccccgc cggaaggatt gagcgccccg ccttcttcgt tggtaaagct cgttccgtgc
     3181 attttcagcg gaccggtgat ccgttcgcgc atcaggcggt cgaatgattt cttcgagacg
     3241 atctccagca cccgggcggc gatattcggc cccaggttac tataaaagaa ttcagtactg
     3301 ggattggtgg cgatatcgtg tttgttggcg tagctgttga ccatgtcttc caggctctcg
     3361 aatttctgtt tcgtcaatac gcttgtcacg ctctgatctg tttttatacc cgtggtattg
     3421 gtcagacagt tccggatcgt gatatatccc ttcatatact tcccgaacag cgggatatac
     3481 tgggtcacct tatcgtcgag ggagagcttg ccttcgtcca caaaggtcat caccagggcc
     3541 gccgtcatcc agttgcccgc gccgtaaatg ggcgcctggc tcttggcggt aaaatcctgg
     3601 ctggcctgtt tctgatagat gaccttccca tccttccaga caagcatcac atatcccccg
     3661 ccaaaggcct tttggttttg cttaagtaat tgatctacag catcaaaatt gggttgggca
     3721 aagctgtgca gacagaatag caggaaaagc gtgaacaaac tgaatcctcg gcaggttaaa
     3781 tggcgcaggt atgacatatt atcgggatta aaggcctggt ttagaatttg caacacaagt
     3841 ttactattta ttacttataa aaaggaaaac catataaaac tatgaacctg aataatttta
     3901 caatcaaggc ccaggaggcg atcgcaaggt cgcagcaact ggccttcgac ggttcaaacc
     3961 ctgccataga tacggatcat cttctgaagg cgttgctcaa cgaagaagat tccctggtgg
     4021 agtttctgct caagaagaat aatgtgaacg tgg
//
LOCUS       DATGFG010000229         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_536297, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000229 DATGFG010000000
VERSION     DATGFG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536297"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="VKQ52_04230"
     CDS             <1..1637
                     /locus_tag="VKQ52_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLZ86422.1"
                     /translation="PTFDVYQGGKFAGIQQQLDYIKGLGAGAIWLSPVLKNLPWDVHS
                     YHGYGIHDFLRADPNFAQDKTKADDELRALVDAAHAKGLYVIFDIVLNHTGDVFQYAG
                     NPNPSFSPTPLPVAWRDALGDAIPAFTDVATIPGPSTDSIVWPAELQSNTYFRRQGPP
                     QAGGNDTIGDFASLKQLRTDLPDVQRSLIRAFQYVIARYDIDGFRIDTLRYLQGGLAL
                     TFGNAVREYGLVLGKKNFFTFGEILDATSESDIAAIIGRNTLLSSDGGNSPVGVDAAL
                     DYPLFNTLVPVAKSSNPPTAVTDMYRIRKSKEQNVISSHGDATRYFVTFLDNHDMKHR
                     FHFVQPGDEHRYDDQLTMALACLYCLPGIPCVYYGTEQGLHGQATPTQNNDAVVREAL
                     WGITPDFPQDAPFYVAMQQIATVRNAQPALRYGRFYFRPVSGDAFHFGISPFPGGILA
                     WSRILNDEEVLIVANTNTSITQPVDVILEWVLSNNGQAVRILYSNKAKPTPPTAIRTL
                     SPVQVTEVDGTVGTGPIHVTHVTLQPMEVQVLRVEQ"
     gene            1804..2178
                     /locus_tag="VKQ52_04235"
     CDS             1804..2178
                     /locus_tag="VKQ52_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86423.1"
                     /translation="MKKVLGFIALMAITNLFQTINAQSKLDFKFCNNTGNTLYSIFVS
                     EHDARTWGKDILKEDVLGNGDCVDVTFPDYGDRTCHWDIKVTYSVDDAQAWTINFVNL
                     CEIRKLTLYRSERDGSVMYKTE"
BASE COUNT          540 a          715 c          560 g          553 t
ORIGIN      
        1 atcctacctt cgacgtctac cagggtggga aattcgcggg catccaacag cagctggact
       61 acatcaaagg cctcggcgcc ggcgccatct ggcttagccc ggtgctcaag aacctgccct
      121 gggacgtgca ctcctaccat ggttatggca tccacgactt cctccgggcc gacccgaatt
      181 tcgcacagga caagaccaag gccgatgacg agctgcgtgc gctggtcgat gcggcacatg
      241 ccaagggact ctacgtcatc ttcgacatcg tcctcaacca caccggggat gtcttccaat
      301 atgccggcaa tcccaacccc tccttttcgc ccactccgtt gcccgtcgcc tggcgtgacg
      361 cgttgggcga tgcgataccg gcctttaccg atgtggccac tattcccggt ccgtcaacag
      421 actccatcgt ctggcccgca gaattgcaaa gcaacaccta tttccgtcgc cagggtccgc
      481 cccaggccgg cggcaacgac accatcggcg acttcgcttc gctaaaacag ttgcgcaccg
      541 atcttcccga cgtccagcgc tccctcatcc gcgcctttca atatgtcatc gcgcgctatg
      601 acatcgacgg gttccgcatc gacaccctgc gttatctcca gggtggcctg gcgctcacct
      661 tcggcaatgc cgtgagagaa tatggattgg tgctcgggaa gaagaatttc tttacgttcg
      721 gggagatcct cgacgctacc tcggaatccg atatcgccgc catcatcggc cgcaacaccc
      781 tcctctcatc agatggcggg aacagccccg tcggggttga cgccgcgctc gactaccccc
      841 tctttaacac ccttgtcccc gtggccaagt cgagcaaccc accaacggcg gtcacggata
      901 tgtataggat ccgtaagtcg aaggagcaaa atgtcatcag ttcgcatggc gacgccaccc
      961 gctacttcgt caccttcctc gacaaccacg acatgaagca tcgcttccat ttcgtgcagc
     1021 cgggtgacga acaccgctat gacgatcagt tgacgatggc gctcgcctgt ctctattgcc
     1081 tgccgggcat tccctgtgtc tattacggta ccgaacaggg gctacatggt caggccactc
     1141 ccacccagaa caatgatgcg gtggtacgcg aagcattatg gggcatcacc cccgactttc
     1201 cccaggacgc ccccttctat gttgcgatgc agcagatcgc caccgtccgc aatgcccagc
     1261 ccgccctgcg ttatggccgt ttctatttcc gccccgtctc gggcgacgcc ttccatttcg
     1321 gcatctcccc ctttcccggc ggcatcctgg cctggagtcg catcctcaac gacgaggagg
     1381 tgctcatcgt cgccaatacg aatacctcga tcacccagcc cgtcgacgtc atcctggaat
     1441 gggtgctcag caacaacggt caagccgtcc gcatcctcta tagcaacaag gcaaagccca
     1501 ctcctcccac cgccatccgc accctctcgc cggtgcaggt gaccgaggtg gatggcacgg
     1561 tcggtacggg accgatccat gttacgcatg tgacgctcca gccgatggag gtgcaggtgt
     1621 tgagggtgga gcaatgacct tagtgtcttc cccctttttt agggattgcc gttattttct
     1681 cctgacacaa acgggaaaaa cactcttttt atcattgccg gctccgccta cctttcggta
     1741 caatcggtaa tggagatctt agccattaca ttcctttttt acacactatt caaacttaca
     1801 attatgaaaa aagtactagg ttttattgct ttaatggcaa ttactaattt attccagacg
     1861 atcaacgctc agtcaaagct tgattttaaa ttttgcaaca acaccggcaa tacactttac
     1921 tcgatttttg tgtctgaaca cgatgcaaga acctggggaa aggatatctt aaaagaggat
     1981 gttctcggta acggagattg tgtcgatgta acctttccgg attatggaga cagaacctgc
     2041 cattgggata ttaaagtcac ttatagtgtg gatgatgccc aggcctggac gataaacttt
     2101 gttaatctat gcgaaatcag gaaattaaca ttgtacagaa gtgagagaga tggttcagta
     2161 atgtataaga cggaataata gttcgttttt tccggagtca ccgggaatac tttacaccaa
     2221 caaaaaaata accttatgcg acatatccaa aagctcaggc ttctcctgct gctatgcacg
     2281 ctgtatagcc aaagctcgtt ttcacaacag gttcaataca taggcattcc acggaatgtc
     2341 tttaatttct atgctgccgc acagcgga
//
LOCUS       DATGFG010000230         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1688396, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000230 DATGFG010000000
VERSION     DATGFG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688396"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..776
                     /locus_tag="VKQ52_04240"
     CDS             321..776
                     /locus_tag="VKQ52_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86424.1"
                     /translation="MKQFVKKAAVVMACVFALSTSYSFAATSGDEGNKEIKASFRHDF
                     ASAQLMSTETHDNFTKMVFTLNGQIMTAFYSTNGDLLAITRNIVSSQLPVNLLMSFKK
                     HYGDYWVTDLFEMSQDAQSNYYLTLENANSRMTLRSNGDNWEVYSSVKK"
     gene            complement(779..1744)
                     /locus_tag="VKQ52_04245"
     CDS             complement(779..1744)
                     /locus_tag="VKQ52_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HLZ86425.1"
                     /translation="MLPAKQSALWRFALSGLDREQPVLLLYVVESKGSSPGRQGFFMV
                     VNGAGEKEGSVGGGIMEHKFFELAREYLRQNTPVNMLRRQVHDKETAKDQSGMICSGE
                     QTILLLSLQAKDKPAIQRLLDQQDNGTLQFTPQGMTVGADPPDTDFYFQQTTGTEWLY
                     REKTGYTTRLYIIGGGHVALAFSRLMQMMDFHITLFDDRSGLDSFHRNEYVQEKRLIE
                     NYSQLEQYITGGPNIFVVVMTQGYRTDDQAIRALWQKEFRYFGVLGSKAKVEKLLSDY
                     RLEGISEERLGKLRAPAGLAIHSQTPEEIAVSIAAEIIRHKHASG"
     gene            complement(1731..2258)
                     /locus_tag="VKQ52_04250"
     CDS             complement(1731..2258)
                     /locus_tag="VKQ52_04250"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLZ86426.1"
                     /translation="MHFDYTKEKGHPFAYHTYGTAIVVTTLDCLRGRYTIDAVKVVHD
                     GGASMNLSIDRGQIEGGIVQGIGWMTMEEVVYDEKGRLRSNTLSTYKIPDIYSAPREI
                     NINFLHTDRDNLAILHSKAVGEPPLMYGIGAYFALRNAMRAFNPAADLPFNAPLTPEK
                     ALMGLYQNRLRHVAG"
     gene            complement(2542..2895)
                     /locus_tag="VKQ52_04255"
                     /pseudo
     CDS             complement(2542..2895)
                     /locus_tag="VKQ52_04255"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
BASE COUNT          600 a          696 c          874 g          741 t
ORIGIN      
        1 gcaagggacg gccgctggtg tacggacaag ataaaatcca gtaccttagt cctgaacggc
       61 aactatcccc ttaaaaaggc acctgtatag ccgttctcag gatttattct ttcacctgta
      121 tagctatttt aggacgagac aaaaccgtct cgaaaccgga cactatttct atcaataccg
      181 gacactcacc ttttcccgcc ttttatcaat tggttgatta tcaattaaga aacaaagttg
      241 gcattccaat ggaactgtct atggcatgcc ggggacgcct ggtagaaaca aaaaatctca
      301 accaatcaaa aaataatttt atgaaacagt ttgtaaaaaa agccgccgtt gtgatggctt
      361 gtgtattcgc ccttagtact tcttatagtt ttgccgctac ttccggtgat gaaggtaaca
      421 aagagatcaa ggcttctttc cggcatgatt tcgcgagtgc ccagttgatg agcacggaga
      481 cccatgacaa ttttaccaag atggtcttta cgctgaacgg acagataatg accgcctttt
      541 attcgaccaa tggcgatctg ctggccatca cccggaacat cgtttcttct caactgcctg
      601 tcaacctgtt gatgagcttt aagaagcact atggcgacta ttgggtgacc gacctcttcg
      661 agatgagcca ggatgcgcag agcaactatt acctcaccct ggaaaatgcc aatagccgga
      721 tgacgctgcg cagcaacggg gataactggg aagtttactc aagcgttaaa aaataggatc
      781 aaccggaagc atgtttgtgg cggatgatct ccgccgcaat gcttaccgca atctcttcgg
      841 gtgtctggct gtggatagcc agtccggccg gcgcccgaag ctttcccagc cgttcttcgc
      901 taatcccttc caatcgataa tccgagagta gtttttccac ttttgccttg cttcccagga
      961 caccgaaata ccgaaactcc ttttgccaca gggcccggat agcctgatca tcggtgcgat
     1021 aaccctgggt catcaccacg acaaagatgt tcggaccgcc ggtgatgtat tgttcgagct
     1081 ggctatagtt ctctattaat cttttttcct gtacatattc gttgcgatgg aaggagtcca
     1141 gaccgcttcg atcgtcgaac agggtgatat ggaagtccat catctgcatg agccgggaga
     1201 aggcgagtgc gacatgaccg ccgccgatga tgtataaccg ggtagtgtag ccggtctttt
     1261 ccctgtagag ccattccgta ccggtcgttt gctgaaaata gaagtcggta tcgggcgggt
     1321 ctgcgccgac ggtcatgcct tgtggggtaa attggagcgt gccgttgtcc tgttgatcga
     1381 ggagtcgttg aatagcgggt ttgtcctttg cctgcaggga gaggaggagg atcgtttgtt
     1441 cgcccgagca gatcatgccg ctttggtcct ttgccgtctc tttgtcgtgg acctgccggc
     1501 ggagcatatt taccggggta ttttgccgga ggtattccct tgccagttcg aagaatttat
     1561 gttccatgat acctccgccg acggagcctt ctttttcgcc ggcaccgttc accaccatga
     1621 agaatccctg tcttcccggg ctgctgccct tgctttccac cacatataat aacaatacgg
     1681 gctgctcccg gtccaggccg gacagggcaa aacgccagag agcggattgt ttagccggca
     1741 acatgcctga ggcgattttg gtatagtccc atcagcgctt tttcgggcgt gagcggggcg
     1801 ttgaagggca ggtccgccgc cgggttaaaa gcgcgcatcg cgttgcggag ggcaaaatac
     1861 gccccgatgc catacataag gggtggttca cctaccgctt tgctgtgtaa gatggcgagg
     1921 ttgtcgcgat cggtgtggag gaagttgata tttatttccc ggggggccga atagatgtcg
     1981 gggatcttgt atgtggacag ggtgttggag cgcaggcggc ctttttcatc atagaccact
     2041 tcttccatgg tcatccagcc gatgccctgg acgatgccgc cctcgatctg gccccgatcg
     2101 atcgagaggt tcatgctggc gccgccatcg tggacgactt ttacggcatc gatggtatag
     2161 cggccgcgga ggcagtcgag agtggtgacg actatcgccg ttccgtaggt gtgataggcg
     2221 aagggatggc ctttttcttt tgtatagtcg aaatgtattt gaggcgtcgc atagtgtgaa
     2281 tgttcggagg tttacgcgcc ggatataggc ggccatgacg agttgcttcc aggtgagggt
     2341 ggtcggggcg ccgttggcgt ggacgaggcc gttgtggatg gtgatggcgt cgggagtgat
     2401 tccctgttcg gcggggttgt cgacggtgtt gggttgtgga gcagtgttgg gttggccggc
     2461 ggagttggat tgtggggcgg agtggttggt ggcgttcagt tcggcggcgg ctacggattt
     2521 cagccgttcg aggatggtgg tacaggcgag ctggacggcc ttgccattga ggtcggcggt
     2581 ggcgctggcg gcgctggggg aggtgttggc gatgcgttgg gtgctggtgg cctgtatgcg
     2641 gaccagggaa gggtcgaggg aaaagacggt ggccgccacc tggatcatct tcgaattgac
     2701 cccctgcccc atttcgaccg cgccggtgct gagggagacg ctaccgtcgg tatagacatg
     2761 gacgagggcc cttgcctggt tgaggagggt ctttgtgaag gagatgccga agcagacggg
     2821 catgaaggcg aggccttttt ttgtgaggcg gtgttttgca ttgaattcgg cgacctgttt
     2881 ttcgagactg tcgatatcgt agagttcctc c
//
LOCUS       DATGFG010000231         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1676214, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000231 DATGFG010000000
VERSION     DATGFG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676214"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VKQ52_04260"
     CDS             complement(<1..769)
                     /locus_tag="VKQ52_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86427.1"
                     /translation="MRAARTIFLFVLTLVARQAVTAQLLTENPLFPVDASTVTITVDC
                     SLGNQGLLGYAATGDVYVHVGVITSASTSSADWRYVPFTWGTTNPAAHAVPVAPNQYQ
                     YTISNIRSFFGVPAGEKILKIAILFRNGNGSLKQANSDGSDMYLPVYDGTLASRYLLP
                     PFQPTYTPIPQPIVKSVGDSLTVEYAASKAVNFGLYFNGTRVDTVNGQDSVKTVLHFG
                     TPGNQQVIATANDGTTVRADTLSFYVAAATTLASPPAG"
     gene            complement(781..2244)
                     /locus_tag="VKQ52_04265"
     CDS             complement(781..2244)
                     /locus_tag="VKQ52_04265"
                     /inference="COORDINATES: protein motif:HMM:NF025650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HLZ86428.1"
                     /translation="MLSIIRSINRRILRTYILATTLCALAAACSKVGNLPTTSFVNGK
                     SPVLTDTAKIMTPQPADSGSVALILSWSNPQYATDSSTEKYIIEIDSSGRNFAKEATI
                     VVTGARRYSFTAKQFNAILLGFGFFYNTGYKVDIRITSSYANNNQPLQSNTVTLNFTT
                     YVVPPKVAPPSSKSLFLVGSASSGGWNNPVPTPAQQFTLVDSVHYKGVFFLNGGQQYL
                     LLPVNGDWSQKYAVQDNTVAGLSAGGVFGYNASSQSVYNANFPGPAKTGLYTITVDFQ
                     QGVFTCTLVNQYGLLFVPGDYQGWTPASAPALGAPKNDGNYDGYVNIPSGGTYQFKFT
                     GEADWNGTIYGDTAASGKSGVLGGGNNLQVAGAGYYHLTANTTANTWSAQATTWSLIG
                     SFAASGWSTDVDMTYNSGSNSWTGTITTAAGDQFKFRANHDWTVNLGDAGGTAVGSLS
                     YGGNNIGDQSKNFSVPAGIHTITLYLGNPGYYSYMIQ"
     gene            complement(2278..3153)
                     /locus_tag="VKQ52_04270"
     CDS             complement(2278..3153)
                     /locus_tag="VKQ52_04270"
                     /inference="COORDINATES: protein motif:HMM:NF025650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HLZ86429.1"
                     /translation="MNSILNNTLLATLFSVLALAACKKQGTDVVYEGGTAPVLTATLK
                     DSIPLSSANAKNQAIGFAWTNPNYQFSNGVSSLNVTYNLEFDTVGANFTSPHMQTVQV
                     SPDLSTSFTVDALNSLVANGLQLAYDQPHTLQVRVVSQMAPYTSGSAGVAVLNSNPLS
                     FTVTPYAPPPAVTPPASGTLVLVGGDPLLGAWSNPVPAAQQFTKVSNTDYQLTIALSG
                     GDPTNSNSSDQYLILPVNGSWSHKYACASTSSQPFSGGSFGLDKSSNFPGPTAAGTYK
                     FDVNFQTGIITVTKQ"
     gene            complement(3181..>4539)
                     /locus_tag="VKQ52_04275"
     CDS             complement(3181..>4539)
                     /locus_tag="VKQ52_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86430.1"
                     /translation="DEAVCAWGDPGVPDFHNMSWTSADPILIGLYARSLYQITLSNDY
                     ITHCTTAAYKSAGIAGADTTKIAGFRAEARFLRAFQYAILMDAFANPPFATETTPVGP
                     LSFPKQIKRADLFKYVESELLACATTLPAPHGNVYGRADQAAAWALLARLYLNAGVYT
                     GTTRYNDAITWSSKVIGAQYTLMPHYANLFQADNDVKNTETILAIPYDGINTQTYGGT
                     TFLVNSACVNSTYTPGSPGYAMSSANYGVPSGGWGGNRSTASLPGLFPTADTTVDKRG
                     IFLNSPGVTKDIASIAQFNQGIPVNKFTNLTSAGVEAEPNAGGTFASTDFPLFRLAEQ
                     YLIYAEAVLQGGTGGDAATALGYINQLRQRAYGSNSGNITSAQLTLPFILDERGRELY
                     WECFRRTDLIRFNEFTGSSYLWPWKGGVASGTAVDAHYAIYPIPVTDLQANSNLVQNP
                     GY"
BASE COUNT          897 a         1146 c         1514 g          982 t
ORIGIN      
        1 tccccgcggg cggtgaggcg agggtggtgg cggcggccac ataaaaactc agggtgtccg
       61 cacgaaccgt tgtcccatcg tttgccgtcg cgatgacctg ttggttgccg ggcgtgccga
      121 aatggaggac ggttttcacc gaatcctggc cgttcaccgt atccaccctt gtcccgttga
      181 agtacaggcc gaaattcacc gccttgctgg ccgcatattc caccgtcagg gaatctccca
      241 cggatttgac gatgggctgg ggaatgggtg tataggtcgg ctgaaagggc ggcagcaaat
      301 aacgactcgc cagcgtgccg tcatacaccg gcaggtacat atcgctgccg tcgctgttgg
      361 cctgtttcag tgagccgttg ccgttgcgga agaggatggc gatcttcagg atcttctcgc
      421 ccgcgggtac gccgaagaag ctgcggatat tgctgatcgt gtactggtac tggttggggg
      481 cgacgggtac ggcatgggcc gcgggattcg tggttcccca ggtgaaaggc acataacgcc
      541 agtcggcgga gctggtgctc gcgctggtga tgacacctac gtgcacatag acatcccccg
      601 tggccgcata gcccagcaag ccctggttgc ccagcgaaca atccaccgtg atggtgaccg
      661 tacttgcatc caccgggaag agcgggttct ccgtcagcag ctgggccgtt acggcctggc
      721 gggcaacgag ggtaaggaca aaaaggaaaa tggttcttgc ggctcgcatg cggtacgggg
      781 ttactggatc atgtaggaat aataccccgg attgccaagg tatagggtga tggtatgtat
      841 gcccgccggc acggagaaat tcttcgactg gtcgccgata ttattcccgc cataggaaag
      901 gctgcccacg gctgttcccc cggcatcccc gagattgacg gtccagtcat gattggcgcg
      961 gaacttgaat tgatcgccgg ccgctgtcgt gatggtccct gtccagctgt tcgagccgga
     1021 attgtaggtc atatcgacat cggtggacca gccgctggct gcaaaactcc cgatgagact
     1081 ccaggtggtg gcctgcgctg accaggtgtt cgcggtggtg ttggcggtga ggtggtaata
     1141 gccggcgccg gccacctgca ggttgttacc gccgccgagg acgcctgact tgccgctggc
     1201 tgcggtgtcg ccatagatag taccgttcca atccgcttcg ccggtgaatt tgaactggta
     1261 cgttccgccg gatgggatat tgacatagcc gtcatagttg ccgtcgttct tcggcgcacc
     1321 gagagccggc gcggaagcgg gtgtccagcc ctggtaatca cccggtacaa agaggaggcc
     1381 gtattggttt accagcgtgc aggtgaagac accctgctgg aaatccaccg tgatcgtgta
     1441 gaggcccgtt tttgcgggac cagggaagtt cgcgttgtac accgactggg aagaggcatt
     1501 gtaaccgaag acgccgccgg cgctgagtcc tgcgaccgtg ttatcctgca ccgcgtattt
     1561 ctggctccag tcgccattta caggcagcag caaatactgc tggccgccgt taaggaagaa
     1621 gacaccctta tagtgtacgg aatccaccag ggtgaactgc tgggccgggg tcggcacggg
     1681 gttgttccag ccaccggagg aagcgctgcc gacgaggaac agggacttgg aggagggtgg
     1741 tgctactttt ggcggcacca cgtaggtggt gaaattcaac gtcaccgtgt tggactgcaa
     1801 tggctggttg ttattggcgt aggacgaggt gatacggata tcgaccttat agccggtgtt
     1861 gtaaaagaac ccgaaaccca gcaggatcgc attgaattgt ttagccgtga aggaataacg
     1921 cctggcgccg gtgaccacga tggtggcctc tttggcgaag ttgcggcccg aggaatcgat
     1981 ctcgatgata tatttttccg tcgaggaatc ggtagcgtat tgcgggttcg accagctaag
     2041 gatcaatgct acgctgccgg agtccgccgg ttggggtgtc atgatctttg ccgtatcggt
     2101 aagcaccggt gactttccgt ttacgaaact ggtagtaggc agattaccta ccttgctaca
     2161 ggcggccgcc agggcacaga gcgtggtggc caggatatag gtgcgaagga tgcgccggtt
     2221 aattgatctt atgatggaaa gcatgttcgt tgattgaatg tttcgataat cgtcgaatta
     2281 ctgtttggtg accgttatga tgccggtttg gaaattgaca tcgaatttat aggtacctgc
     2341 ggcggtcggt cccgggaagt tggaactctt atcgagcccg aagctgccgc ccgagaaggg
     2401 ctgactggac gtgcttgcac aggcatattt atgactccag ctgccgttga cgggcaatat
     2461 caggtattga tcgctgctgt tggaattggt cggatcgccg ccggagagcg caatggtaag
     2521 ctggtaatcc gtattgctga cctttgtgaa ctgttgcgcc gccggaacgg ggttggacca
     2581 ggcgcccaac aacggatcgc cgccgaccag caccagggtt cccgaagcag gaggtgtcac
     2641 ggcaggtgga ggggcatagg gggtaacggt aaaactgagg ggattggagt tgagcacggc
     2701 gacaccggcc gagcccgagg tatagggagc catctgggag accacgcgca cctgcagggt
     2761 atgcggctgg tcgtaggcta gttgcaggcc gtttgcgacg aggctgttca aggcgtccac
     2821 ggtgaacgac gtagacagat cggggctcac ttgcaccgtc tgcatgtggg gactggtgaa
     2881 attggcccct accgtatcga actcgagatt ataggtgaca ttgagggagc taacgccgtt
     2941 ggagaactgg taattgggat tcgtccaggc gaaaccgatc gcctggttct ttgcgttggc
     3001 ggagctgagg gggatggaat cctttagcgt ggcggtcagc acgggcgccg taccaccttc
     3061 gtacacaaca tcagtgccct gcttcttgca cgccgcgagg gccagcacgc taaagagagt
     3121 ggcgagtagt gtattattga gaatgctgtt catgagcgtt tcattttttg attgtaggaa
     3181 ttaatacccg gggttttgaa ccaggttgga gtttgcctgg agatcggtca ccggtatggg
     3241 atagatggca tagtgcgcat cgacggccgt accgctggct accccaccct tccagggcca
     3301 cagataactg ctgcccgtga actcgttgaa tcggatgaga tcggtgcggc ggaaacattc
     3361 ccaatacaat tcccggcctc tttcatcgag gatgaagggc agggtcaact gggccgatgt
     3421 gatattcccg gagttgctgc cataagcgcg ctggcgcagc tggttgatat accccagggc
     3481 cgttgccgca tcgccgccgg tgccgccctg caggacagct tccgcataga taagatactg
     3541 ttccgccaga cggaagaggg ggaaatcggt agaggcgaac gtgccgccgg cattgggttc
     3601 cgcttcgacg ccggccgatg tgaggttggt gaacttattg accggaattc cctggttgaa
     3661 ctgggcgatg ctggcgatat cctttgttac accggggctg ttcaggaaga tgccgcgctt
     3721 atccaccgtc gtatcggcag tgggaaagag acccggcaga ctggcggtgc tgcggttgcc
     3781 gccccagccg ccgctgggaa ccccgtagtt ggcggagctc atggcatagc cggggctgcc
     3841 cggcgtatag gtagagttga cgcaggcact gttcacgagg aaggtggtgc cgccgtaggt
     3901 ctgggtgttg atcccatcgt aggggatggc caggatcgtc tccgtgttct ttacatcgtt
     3961 gtcggcctgg aagagattcg cataatgggg catcagggta tattgtgcgc cgatgacttt
     4021 ggatgaccag gtgatggcat cgttgtagcg ggttgtcccc gtgtatacgc cggcgttgag
     4081 atagaggcgg gccagcaatg cccaggcggc tgcctggtcg gcccggccgt atacattgcc
     4141 gtggggcgct ggcagggtcg tggcgcaggc gaggagttcg gattcgacgt acttgaagag
     4201 atcggcgcgt ttgatctgtt tggggaagga gagcgggccc accggggtcg tctctgtggc
     4261 gaagggcgga ttggcgaagg catccatcag gatggcatac tggaaggccc ggaggaaacg
     4321 ggcttcggcc cggaacccgg cgatcttcgt agtatcggcg ccggcgatgc ctgccgattt
     4381 gtaagcagcg gtggtgcaat gggtgatata gtcattggaa agcgtgatct ggtagaggct
     4441 tcgtgcatag aggccgatga ggatggggtc ggccgatgtc cagctcatgt tgtgaaaatc
     4501 gggcacgccg ggatcgcccc aggcgcagac ggcctcgtc
//
LOCUS       DATGFG010000232         5182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1101002, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000232 DATGFG010000000
VERSION     DATGFG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101002"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..980
                     /locus_tag="VKQ52_04280"
     CDS             63..980
                     /locus_tag="VKQ52_04280"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HLZ86431.1"
                     /translation="MQLKKYCLTFALFSLFLCSARAQDFFKPSTQYNPGRVNGTIIVE
                     SAIGVAVTIGLNYLWYKKFAHGKFHYFNDNNEWLNVDKVGHATTAYNIAAIQSDVLRW
                     GGVRNGTSALIGTATALGFMTMIEIMDGHSEKWGFSKGDMLANIAGCLLFEGQQLMWG
                     QQRISLKYSYHGTIFPSYQPAELGRNLPQRMLKDYNGQSYWLSFNIASFLPASAHFPQ
                     WLNLSAGYGAEGMIGAVSNPREIDGKPIPSFRRYRQFYLSFDTDLYRIDGLSPLAATL
                     LKVNRTFKTPAPALEWNELNGVKFHIFYY"
     gene            1085..4243
                     /locus_tag="VKQ52_04285"
     CDS             1085..4243
                     /locus_tag="VKQ52_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HLZ86432.1"
                     /translation="MNKVFKGILAFSLKNKYFIFFATLLLVIYGVITFRNMPIEAFPD
                     VTNTEITIITQWPGRSAEEVEKFVTIPIELAMNPVQRKTSLRSTTIFGLSVVKLIFDD
                     DVQDTYARQQVNNLLHNADLPEYVDPNVQPPTGPTGEVFRYTLESTFRDPRELKTLQD
                     WVVDRELRSVPGVADIVSFGGMVKTYEIQVNPLVLNNLGITPLDVYNAVSKSNINIGG
                     DVIKKNSQAYVVRGIGLLNDINEIKNIIVENNNGIPVLVKDVAEVQISNLPRLGEVGR
                     TDATDTTGHRRLRNNPDAVEGIVLMRKGENPDHVIQGIKTAVQRLNERILPADTKMIP
                     FYNREDLIKYATHTVLHNLVEGILLVTLIVSLFMFNWRTTLIVSIIIPLSLLFSFICL
                     NYMGMSANLLSLGAIDFGIIIDGAVIMVEGLFVVLDKRAHEVGMERFNHLSKLGMIRR
                     RGSELAKAIFFTKIIIITALLPIFSFQKVEGKLFSPLAYTLGFGLLGALIFTLTLVPV
                     LVNVLLRKNVKEKHNPIVHFLTGIVMAVFTFNFHNKRLTLIVTSIIIAIGLYAFKFLG
                     SEFLPELNEGSIWVRATLPYSISLDTSMSKAEQMRAIMIKFPQVKRIMSQTGRPDDGT
                     DVAGFYNNEFGVSLYPQEEWDPKITKEELIDTMSRALSVIPGADLNFSQPIMDNVEEA
                     VSGVKGSICVKVYGDSLDYMEEQSTTVYNVLKKIAGIDDLGVIRNIGQPELDIDLNQS
                     KMALYGVSTADANAVIEMAIGGKAVTQLYEGIRKFDIRIRFPEQFRMTQDDIGNLLVP
                     TLSGSKVPVHEIATISQKTGPCLIFRDDNRRYSAVKFSVRGRDMGSAIAEAQEKVDQV
                     VHLKKGYSMVWQGDFENQQRASKRLAQVVPISLLLIFLLLFVMFGNLKDAGLVFLNVP
                     TAVVGGILALLITGTNFSISAGIGFIALFGICILTGVLLITAFKHNLELIRHSESPLY
                     TSIKLGVNELIRPVLMTALMATIGLLPAAISTGIGSESSRPLARVVIGGLICATVFSL
                     LIFPLVFFLAYRKVDRLHTKEG"
     gene            4286..4813
                     /locus_tag="VKQ52_04290"
     CDS             4286..4813
                     /locus_tag="VKQ52_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86433.1"
                     /translation="MDSNMLIIAVAFATLVAGVLVFLFLKQRRELKAAQAAQAAGPAT
                     GDAGTRQLQLQAYERLILLTDRIALPNLIQRLNQPGLEARDMQALLTQGIRQEFEHNI
                     TQQIYVSAEAWEAVRNYKEQNLLIINQVSSFLPPEATGIDLNKHLLDLLVQNPKASLQ
                     NVVSEVLSFEAKKLM"
     gene            4823..>5182
                     /locus_tag="VKQ52_04295"
     CDS             4823..>5182
                     /locus_tag="VKQ52_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLZ86434.1"
                     /translation="MPSKRYTDSGIEVKEVYTAADLPGADSHLPSGPAAGSELPGEFP
                     YTRGVQSDMYRGKLWTMRQYAGFSTAEESNARYHYLLSQGVMGLSVAFDLPTQIGYDS
                     DHPLSEGEVGKTGVAIDS"
BASE COUNT         1168 a         1560 c         1334 g         1120 t
ORIGIN      
        1 cttctacaac aagcgaccgc tacattttta accaaagcgg tcgctttttt ttatcttcat
       61 gcatgcaatt gaaaaaatat tgcctgacgt tcgccctttt ctctctcttt ctatgctccg
      121 cccgggcaca ggatttcttc aaaccgtcga ctcaatataa ccccggccgg gtcaacggca
      181 ccatcatcgt cgaatccgcc atcggcgtcg cggtcaccat cggcctcaat tatctctggt
      241 ataaaaaatt cgcccacggc aaattccact acttcaacga caacaacgaa tggttgaacg
      301 tcgacaaggt gggccacgcc acgacagcct ataatattgc cgccatccag agcgatgtcc
      361 tgcgctgggg cggggttcgc aacgggacat ccgcccttat cggcaccgcc accgcgctgg
      421 gttttatgac catgatcgag atcatggacg gacattccga gaaatggggg ttctccaaag
      481 gagacatgct cgccaatatc gcgggttgcc tcctcttcga aggacaacag ctgatgtggg
      541 gccagcagcg tatcagcctg aaatattcct accatgggac catcttcccc agctatcagc
      601 cggccgaact gggtcgcaac cttccccaac ggatgctaaa agactacaat ggccaaagct
      661 actggctgtc cttcaatatc gcctcctttc tcccggcatc cgcccatttc ccgcagtggc
      721 tcaacctgtc cgcaggctat ggcgccgagg ggatgatcgg cgccgtgagc aaccccaggg
      781 agatcgatgg taagccgatc ccctctttcc ggcgttaccg ccagttctat ctctccttcg
      841 ataccgacct ctatcgcatc gacgggctct cccccctcgc cgctaccctc ctgaaggtga
      901 accgcacgtt caaaaccccc gctcccgccc tggaatggaa tgagctcaac ggggtaaaat
      961 tccatatatt ttactattga tttgttaatg gcggcggaat tttgttaatg ggggctacct
     1021 tcgtcctatt cgttaacaac ggcttaactt ttcattaact tgccggtcct aacactcctg
     1081 actgatgaac aaagtcttca aaggcattct ggctttttcc cttaaaaaca aatatttcat
     1141 ctttttcgcg accctcctcc tggtgatcta tggggttatc accttccgga acatgccgat
     1201 cgaggccttc ccggatgtga ccaacaccga gatcacgatc atcacccagt ggccgggccg
     1261 cagcgcggaa gaggtggaaa agttcgtcac gatccctatc gaactggcta tgaaccccgt
     1321 tcagcggaag accagcctcc gttcgaccac cattttcggg ttatccgtag taaaactgat
     1381 ctttgatgac gatgtacagg atacctatgc ccggcaacag gtaaacaatc ttctgcataa
     1441 tgccgatctc cccgaatacg tagaccctaa tgtgcagccg ccgacgggtc ccacaggaga
     1501 ggtcttccgg tatacgctgg agagtacttt ccgcgatccg cgggagctga agacgctcca
     1561 ggactgggtt gtcgaccggg agctccggtc tgtcccggga gtagcggata tcgtgagttt
     1621 tggcggcatg gtcaagacct atgagatcca ggtgaacccc ctcgtgctga ataacctggg
     1681 gatcaccccc ctggacgtct acaacgccgt cagcaaaagc aatatcaata tcggcggcga
     1741 cgtcatcaag aagaattccc aggcctatgt cgtcaggggt atcgggctgt tgaacgatat
     1801 caacgagata aagaatatca tcgtcgagaa caacaacggt atccccgtcc tggtaaagga
     1861 tgtggcggag gtacagatct cgaacctgcc ccggcttggc gaagtaggcc gcacggacgc
     1921 gaccgatacc acaggtcacc gccgcctgcg gaacaatccc gacgccgtcg aagggatcgt
     1981 cctgatgcgg aagggcgaga accccgacca cgtcatccag gggatcaaga cggccgttca
     2041 gcggctgaac gaacgcatcc tgcccgcgga caccaagatg atccccttct ataaccggga
     2101 agacctgatc aaatatgcca cacataccgt gttgcacaac ctggtggaag ggatcctgct
     2161 ggtgacattg atcgtgtccc tgtttatgtt caactggcgg acaaccctca tcgtgtcgat
     2221 cattattccc ctctcgcttc ttttctcctt tatctgtctc aactatatgg gcatgtcggc
     2281 caacctcctc tccctggggg ccatcgactt cgggatcatc atcgacggcg ccgtgatcat
     2341 ggtggaaggc ttgttcgtgg tgctggacaa acgggcgcat gaggtgggga tggaacgctt
     2401 caatcacctg tccaagctgg ggatgatccg ccggcgggga tccgaactgg ccaaggccat
     2461 cttctttaca aagatcatca tcatcacggc cctgctgccc atcttctctt tccagaaggt
     2521 ggaaggaaag ctgttctcgc cgctggccta tacgctgggc tttggcctgc tgggcgcgct
     2581 gatcttcaca ctgaccctcg tgcccgtgct ggtcaatgtc ctgctgcgca agaatgtgaa
     2641 agaaaagcac aacccgatag ttcattttct tacgggtatc gtcatggccg tctttacctt
     2701 caatttccac aacaagcggc tgaccctgat cgtcacatcg atcatcatcg cgatcggact
     2761 atacgccttt aaatttttgg ggtccgagtt tctgcccgaa ctgaacgagg gttccatctg
     2821 ggtccgcgct accctgccct acagcatctc cctcgatacc tccatgtcca aggccgagca
     2881 gatgcgggcc atcatgatca aattcccgca ggtgaaacgc atcatgtccc agacgggccg
     2941 gccggacgac ggtaccgatg tcgcgggctt ttacaataac gagttcggtg tctcgctcta
     3001 tccgcaggag gaatgggacc ccaaaataac caaggaagag ctgatcgaca ccatgagcag
     3061 ggccttatcg gtcattcccg gcgccgacct caatttctcc cagcccatca tggacaacgt
     3121 ggaagaggcc gtttccggcg taaagggctc catctgcgta aaagtatacg gagactccct
     3181 ggattatatg gaggaacagt cgacgactgt ttacaatgtg ctaaagaaga tcgcggggat
     3241 cgacgatctc ggggtgatcc gtaatatcgg tcagccggaa ctggacatcg acctcaacca
     3301 aagcaagatg gcgctgtatg gcgtttccac ggccgatgcc aatgccgtta tcgagatggc
     3361 catcggtgga aaggccgtga cgcaactgta cgagggcatc cggaaattcg atatccgtat
     3421 ccgcttcccc gagcaatttc gcatgaccca ggacgatatc ggcaacctgc tcgtgcccac
     3481 cctcagcggt agcaaggtac ccgtacacga gatcgcgacc atcagccaga agaccggtcc
     3541 ctgtctgatc ttccgcgacg ataaccgccg gtattcggcc gtcaaattct cggtccgtgg
     3601 ccgggatatg ggtagcgcca tcgccgaggc ccaggagaaa gtggaccagg tggtccacct
     3661 gaagaaggga tactcgatgg tctggcaggg ggatttcgag aaccagcaac gggcctccaa
     3721 acggctggca caggtggtgc ccatcagcct gctgctgatc ttcctgctgc tcttcgtcat
     3781 gttcggcaat ctcaaggacg ccgggctggt cttcctcaac gttcccacgg ccgtcgtcgg
     3841 ggggatcctt gccctgctga tcaccggtac caatttcagc atctcggcag gcatcggctt
     3901 tatcgcgctg tttggcatct gtatcctcac aggggtgctg ctgatcactg ccttcaagca
     3961 taaccttgaa ctgatcaggc acagcgaaag tccgctgtac acttccataa aactgggggt
     4021 gaacgagctg atccggcccg ttttgatgac cgccctgatg gcaaccattg gattattacc
     4081 ggcggcgatc tcgacgggta tcgggtcgga aagcagccgg cccctggccc gggtcgtgat
     4141 cggcggcctc atctgcgcaa cggtattcag cctgctgatc ttcccgctgg tattcttcct
     4201 ggcttaccgg aaagtagaca gactgcatac aaaagaagga taaataataa acgccgcgtc
     4261 agcggcgttt atctattttt gaactatgga ttccaatatg ctcatcattg ccgtcgcatt
     4321 cgccaccctg gtcgcgggcg ttctcgtctt tctttttttg aagcaacgcc gcgaattaaa
     4381 ggccgcgcag gcggcccagg ccgccggacc ggccaccggc gacgcgggta cccggcagct
     4441 ccaactccag gcctatgaac gcctgatcct cctgaccgac cgcatcgccc tgcccaacct
     4501 tatccagcgg ctcaaccagc cgggtctcga ggcccgggac atgcaggccc tgctgaccca
     4561 gggcatccgg caggaattcg aacacaatat cacccagcag atctatgtca gcgccgaagc
     4621 ctgggaggcc gtccgtaact ataaggagca aaacctgctg atcataaacc aggtcagttc
     4681 cttcctgccc ccggaagcca ccggcatcga cctgaacaag cacctgctcg atctcctcgt
     4741 acaaaacccc aaggcatccc tgcaaaacgt cgtgtccgaa gtactcagct tcgaagcgaa
     4801 aaagttgatg tagattcgcg gaatgccatc taaacgatat acggacagcg gtatcgaagt
     4861 caaagaggta tataccgctg ccgacttgcc cggcgccgac tcccatttac catccggccc
     4921 cgccgccggc agcgaactac ccggcgagtt cccttatacc cgcggggtcc aaagcgacat
     4981 gtacaggggg aaattgtgga ccatgcggca gtatgcgggt ttcagcacag ccgaagaaag
     5041 caatgcccgc tatcactatc ttctcagcca gggggtcatg ggactcagcg tcgccttcga
     5101 tctccccacc cagatcggct atgacagcga ccatcccctc tccgaagggg aagtagggaa
     5161 gacgggggtc gcgatcgact cc
//
LOCUS       DATGFG010000233         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_383966, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000233 DATGFG010000000
VERSION     DATGFG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383966"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VKQ52_04300"
     CDS             complement(<1..732)
                     /locus_tag="VKQ52_04300"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ86435.1"
                     /translation="MILRELPSPLALADTISGITVLQADRLRGEWSIPLVARAAPSIV
                     YQSTAGSAQLVLYGQNVRPFALRASGHLTMIAWFLHPHRLGPLFGFRASEVTEICVDL
                     GSLPLVRRLSLVERLGESGEVRECLELMQEFIRPLCAAATGGVNDAAGYATGVLRGGN
                     GLVSLRSLQEELRITERSFQRLFDLHIGVSPKQYSRICQFQPAFRQLSGGQFGRLSDI
                     AYDNGYADQSHLIRVFREFTGLTPKE"
     gene            774..1358
                     /locus_tag="VKQ52_04305"
     CDS             774..1358
                     /locus_tag="VKQ52_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ86436.1"
                     /translation="MTEETAHNDRILLLQMARGDEKGLTGIVRLYWKDIYSQAIVLLK
                     SAEEAEELTQDIFLKVWNARDQLEHIENFANWLFIVARNTIITAFRKKLNRPGFLQEQ
                     EAEEVALRPDRQAELRPDHQAEHREQYRLLLKGISLLPEKRQQVFRLSRMEGLTHEQI
                     AERLGIHKDTVAQYIVKAVAFLRSYLREHQGTPP"
     gene            1414..>2164
                     /locus_tag="VKQ52_04310"
     CDS             1414..>2164
                     /locus_tag="VKQ52_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86437.1"
                     /translation="MHHRLQALLNSYLSGNLTPEQHRELLTLLDDTGAADQLGEQLRQ
                     QISTGDPIIPNELPGTEDRIVRGLLEKIRREPAVAPQPQSDTRPELQTVADAQPGAPL
                     ANPTPVRPLYRRWIAAAAAILIITSGAYLWLAKTKPAPAVATRRTQTDIAPGGNKAIL
                     TLADGKTITLDSTADGAIAQQGNATIIKSANGQIAYQPTGTATADANLLNTLRTPFGG
                     QYHLTLPDGTGVWLNSASAITYPAAFTGRQRK"
BASE COUNT          526 a          799 c          512 g          327 t
ORIGIN      
        1 ctccttcggc gtaagacccg tgaactcccg gaacacccgg atcagatggc tctgatcggc
       61 gtacccatta tcataggcga tatcactaag ccgcccgaac tgccccccgc tcaactgccg
      121 gaacgccggc tggaactggc agatccggct atattgcttt ggcgagaccc cgatatgcag
      181 atcgaaaagc cgctggaaac tacgctcggt gatccgcagc tcctcctgca acgaccgcag
      241 ggacaccaac ccgttgccgc ctcgtagcac cccggtcgca tacccggcgg cgtcattcac
      301 cccacccgtc gcggcggcac acaacggccg gataaactcc tgcatcaact ccaaacactc
      361 cctcacctcc ccggactccc caagccgttc caccaggctc agccgccgca ccagcggcaa
      421 agaacccaga tcgacacaga tctccgtcac ctcactcgcc cggaacccga acaaggggcc
      481 cagccggtgg ggatgcagga accaggcgat catggtcaga tgacccgatg cccgcaaggc
      541 aaaaggccgg acattctgcc catataacac cagctgcgcc gaccccgcag tcgattgata
      601 cacgatggaa ggcgccgccc tcgccaccag cggaatgctc cattcccccc gtaaccggtc
      661 cgcctgcaac accgtgatgc cgctgatcgt atccgccagc gccaacggcg atggcaattc
      721 acgtaggatc atatcccaaa ataaattatc tttcggctca tctgcgagaa aaattgacgg
      781 aagaaaccgc gcataacgac cgcatcctcc tgctccaaat ggcccgtgga gacgaaaaag
      841 gcctcaccgg tatcgttcgc ctgtattgga aggacatcta ttcccaggcc atcgttctgt
      901 tgaagtcggc cgaagaagcg gaagaactca cccaggacat cttcctcaag gtctggaacg
      961 ccagggacca gttggaacac atcgaaaatt ttgccaactg gctcttcatc gtcgcccgca
     1021 atacgatcat caccgccttc cggaaaaagc tcaaccgccc tggcttcctg caggaacagg
     1081 aggccgaaga ggtcgcgctg cgtcccgacc ggcaagccga actgcgcccc gaccaccagg
     1141 ccgaacaccg cgaacagtac cgcctcctac tcaaaggcat cagcctcctg ccggaaaaac
     1201 gacaacaggt ctttcgcctg agccggatgg aagggctcac ccacgaacag atcgccgaac
     1261 gcctcggcat tcataaagac actgtggctc aatatattgt caaggcagtc gccttcctgc
     1321 gcagctactt gcgggaacac cagggaacac ccccataaaa aaaatcgcac cccgcatact
     1381 cagtcggtcc tgcctcccgc gtctttaata gtaatgcatc atcgattaca agctttactg
     1441 aatagttacc tgtccggtaa cctaacaccg gaacagcacc gcgaactgct caccctgctc
     1501 gacgacaccg gggcagcaga tcaactcggt gaacagctcc ggcaacaaat ttccaccggc
     1561 gaccccatca tccccaatga actcccggga acggaagacc gcatcgtccg cggcctcctc
     1621 gaaaagatcc ggcgcgagcc cgctgtcgcc ccgcaacccc aatccgacac ccgccccgaa
     1681 ctccaaaccg tagccgatgc ccaacccggc gcccctctcg cgaacccaac cccggtgaga
     1741 cccctctacc gccgatggat agcagccgcg gccgccatcc tcatcatcac cagcggtgcc
     1801 tacctctggc tcgccaaaac aaaaccggct cccgcagtcg ccacccgaag gacgcagaca
     1861 gacatcgctc ccggcggcaa caaagccatc ctcaccctgg ccgacggcaa gaccatcacc
     1921 ctcgacagca ccgccgacgg cgccatcgcc caacagggca atgccacgat catcaagtcg
     1981 gccaacggcc agatcgccta tcagccaaca ggcacggcta cggcggacgc caacctcctc
     2041 aacacgctgc ggacaccatt cggcgggcaa tatcatctca ccctccccga cggaaccggc
     2101 gtctggctga actccgcatc ggccatcacc tatcccgcgg ccttcaccgg caggcagcga
     2161 aagg
//
LOCUS       DATGFG010000234         4142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_707024, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000234 DATGFG010000000
VERSION     DATGFG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707024"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VKQ52_04315"
     CDS             <1..1235
                     /locus_tag="VKQ52_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HLZ86438.1"
                     /translation="GSAGGFGDWDPVTRTFADPASAIYRAYVRLIGAGAVAMGGLITL
                     IKTIPTIVSSFKESIGSVRAAGPDSRLRTERDLSLRVVGLGSLALVILIGFLTIIPGD
                     SFFNRLLIGVLILLFGAFFVTVASRIVGLIGSSNSPVSGMTIAAVMGTCLIFLAIGWS
                     GKVYEPMALVVGGMVCIAASNAGGTSQDLKTGYLVGATPRYQQLALFIGVIVSSFAVG
                     FTIKILDIPDPELLARGIHHKIGSTFQAPQATLMATLIRGILSKNLDWNFVLAGAALA
                     LVMELCGVRSLSFAIGVYLPFSTTCPIFLGGMIRGLVDRVKKRRGALIKEGEEDLEKG
                     NLFVTGLVAGGALMGVIFAFLKVPQSVVAFMNKLSLEKGLTAVLSENGYYLLGFAFFA
                     VMGYALYRAGTAKEKPIL"
     gene            complement(1230..1559)
                     /locus_tag="VKQ52_04320"
     CDS             complement(1230..1559)
                     /locus_tag="VKQ52_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86439.1"
                     /translation="MKKLPGLLLLILIALQPGCKKSNDVLGVVNAHVLYGGDPVVDGP
                     GYYIHLDGSGENIVALNLPSSYQHPTVNEAVAIKFVDTGKRRIVGFADADHGGWRVVY
                     IVTIRNL"
     gene            complement(1680..2066)
                     /gene="apaG"
                     /locus_tag="VKQ52_04325"
     CDS             complement(1680..2066)
                     /gene="apaG"
                     /locus_tag="VKQ52_04325"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HLZ86440.1"
                     /translation="MANKISEGMEVSVETFYQPDYSNPISGEYMFAYRITIENHNNFS
                     VKLHRRHWHIFDSNGSYREVEGEGVVGVQPILQPGERYQYVSGCNLRTEMGKMYGSYT
                     MENLNNKKSFEVNIPIFEMIVPFKMN"
     gene            2183..3634
                     /locus_tag="VKQ52_04330"
     CDS             2183..3634
                     /locus_tag="VKQ52_04330"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HLZ86441.1"
                     /translation="MKIAVAKGDGIGPEIMDAVLAIFTAARVPLEYTFVDMGKWVFDK
                     GFSNGMTPEAKQTIEELGILFKGPMETPKGKGVKSVNVTARKTWNTYANKRVFQTLHG
                     VDTVFSKAGIPIDITIVRENIEDTYGGIEHMLTQDVALSRRFITRPGSLQVIKYAFEM
                     ARRNGARRITCGHKANIMKLTDGLFLECFQEVAKEYPDLRSDDIIVDDLAMKLVVRPQ
                     DFDVVVLTNLQGDIISDLCAGLVGGLGFAPSANIGDHIAIFEAVHGTAPDIAGRNIAN
                     PTALLLSGISMLRHLGLMENAAIIENALLYTLEQGIHTGDFGDKSTPPLNTTDFARAI
                     IDAFGKLPEHMARPLLPNAPVTPTAFKLSRNEMMVSEETQEEVIVGVDLFIESSGQPE
                     FIAKKCQRHGGVKFNLVSISNRGTQVWPTGSVYTNLVNQYNVRFESIDGSPLNQQDVL
                     GLYVSLSGDFRICSSELLLMWGGRKAYSLAQGQ"
     gene            complement(3749..>4142)
                     /locus_tag="VKQ52_04335"
     CDS             complement(3749..>4142)
                     /locus_tag="VKQ52_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HLZ86442.1"
                     /translation="TKNGPRGPCPKDKQKRQPMSKKKANSNGIVYSTDPDFRVEEESA
                     PQESLLPKQQKLKVRLDTRQRAGKAVTLVEGFIGAAAELEDLGRRLKTHCGTGGSVKD
                     GEIIIQGDQRDKITQWLIAKGFTATKRL"
BASE COUNT          856 a         1211 c         1147 g          928 t
ORIGIN      
        1 tcgggtcagc cggcggcttt ggcgactggg accccgtgac ccggaccttt gccgatcccg
       61 cctcggccat ctatcgcgcc tatgtccggc tgatcggcgc gggagcagtg gccatgggtg
      121 ggctgatcac cctgatcaag accatcccga cgatcgtgtc ctcctttaaa gaaagtattg
      181 gctcagtaag ggcggccggc ccggatagcc ggctacgcac ggaacgcgat ctgagcctgc
      241 gcgtcgtggg gctcggcagt ctcgcactcg tgatccttat cggcttcctg accatcatcc
      301 ccggcgactc cttcttcaac cgcctgctga tcggggtgct gatcctgttg ttcggcgcct
      361 tcttcgtaac ggtggcctcc cgcatcgtag gccttatcgg ctcttccaac agcccggtat
      421 ccgggatgac gattgctgcc gtcatgggca cctgtctgat cttcctggcc atcggctgga
      481 gcgggaaggt ctatgaaccg atggccctgg tcgtcggagg catggtctgt atcgccgcct
      541 ccaatgccgg tggtacctcc caggatctca aaacaggtta tctcgtgggc gccacccccc
      601 gttaccagca gctggcgctt ttcatcgggg tcatcgtctc ctcattcgcc gtcggcttca
      661 ccatcaagat cctggatatc cccgatcccg agctgctcgc cagaggcatt catcataaga
      721 tcggcagtac atttcaagcc ccccaggcca cgctgatggc taccctcatc aggggaatcc
      781 tgtcgaagaa cctggattgg aatttcgtgc tggcaggcgc cgcgctggcc ctcgtgatgg
      841 agctttgcgg cgtgcggtca ttgagtttcg ccatcggcgt ctaccttccc ttctccacca
      901 cctgtcccat cttcctcggc gggatgatcc gcggcctggt ggacagggtg aaaaagcggc
      961 ggggcgccct cataaaagaa ggtgaggaag atctcgaaaa agggaatctc ttcgtaaccg
     1021 gcctcgtcgc aggtggagcc ctgatggggg tgatcttcgc cttcctgaag gttccccagt
     1081 ccgtcgtcgc gttcatgaac aaactcagcc tggaaaaagg cctgacggcc gtgctcagcg
     1141 agaacggcta ctatctgctg ggatttgcct tctttgccgt gatgggctat gcgctttacc
     1201 gggcgggaac agcaaaagag aagccgatac tataagtttc ggatcgtaac gatatacacc
     1261 acccgccacc cgccatggtc ggcgtccgca aaaccgacaa tgcgtcgctt gcccgtatcc
     1321 acgaatttga tggctacggc ttcattgacg gtcggatgct ggtaggaaga gggaagattg
     1381 agggcgacga tattttcgcc gctcccatcg agatggatat aatatcccgg gccatcgacc
     1441 acaggatcac caccataaag gacatgggca ttgactacgc cgagtacatc gttgcttttc
     1501 ttgcaaccgg gttgtaaggc gatgaggatc agcagcagga ggcctggtaa ctttttcatg
     1561 ataagctttc tacccccaat gacaggaaaa cagcgccgac cgttgcgtgc aaactgttaa
     1621 aacgctcccg atccctcggc ggggcggggg ccgattagtt catcctgagc ggaggccgct
     1681 cagttcatct tgaaaggaac gatcatctca aaaatgggaa tatttacttc gaaagacttc
     1741 ttattgttga ggttctccat ggtgtaggag ccatacattt tacccatttc ggtgcgcaga
     1801 ttgcaaccgc tgacatattg atatcgctcg cccggctgga ggatgggttg gacgccaacg
     1861 acaccctcgc cctctacctc ccggtagctg ccgttgctgt cgaagatatg ccagtgacgg
     1921 cggtggagct ttacggagaa attgttgtga ttttcgatgg tgatcctata ggcaaacata
     1981 tactccccgg agatggggtt ggaataatca ggctggtaaa aggtctcgac actcacctcc
     2041 attccttctg agatcttgtt tgccattaac caataatttt tctaaagtta atcaaaatag
     2101 gggaggcttt gcgtaaaaaa ctaacctaat tttgtcgctt gttcccttat aaagggtaga
     2161 aaaaatataa aaattcaaaa atatgaagat cgcagttgca aaaggagatg ggattggacc
     2221 ggagatcatg gatgccgttc tcgctatttt caccgccgcc agggtgccgt tggaatatac
     2281 gttcgtggat atgggtaaat gggtgttcga caaggggttt tccaacggga tgacacccga
     2341 ggcaaaacag accatcgagg aattgggcat cctgttcaaa ggccccatgg aaacgccaaa
     2401 gggaaaagga gtaaaaagcg tgaacgtgac ggcccgcaag acctggaaca cctatgcgaa
     2461 taaacgcgtt ttccagaccc tgcatggagt ggataccgtc ttttcgaaag ccggcattcc
     2521 catcgacatc accatcgtcc gggagaatat cgaagacacc tatggaggca tcgaacacat
     2581 gctcacccag gacgtggcgc ttagccggcg ttttatcacc cggcccggca gtctgcaggt
     2641 gataaaatac gccttcgaga tggccaggag gaatggcgcc cggcgcatca cctgcggcca
     2701 caaggcgaat atcatgaaac tgacggatgg ccttttcctg gaatgcttcc aggaagtggc
     2761 caaagaatac ccggacctgc ggtcggacga tatcatcgtg gacgatcttg ccatgaagct
     2821 ggtcgtgcgg ccccaggatt tcgacgtcgt ggtcctgacc aatctgcagg gggatatcat
     2881 ctccgatctt tgcgccggcc tggtcggcgg cctcggattc gccccctccg ccaatatcgg
     2941 tgaccatatc gccatcttcg aggcggtgca cgggacggcc cccgacatcg ccggccggaa
     3001 tatcgccaac cccacggcgc tgctcctcag cggcatctcg atgctccggc acctcggcct
     3061 gatggagaac gccgccatca tcgagaacgc cctcctgtat acgcttgaac aggggatcca
     3121 taccggcgac ttcggcgaca agagcacccc gcccctgaat acgacggatt tcgccagggc
     3181 catcatcgac gcctttggca aactgccgga acatatggcg cggcccctgc tgcccaacgc
     3241 acccgtgacc ccaacggcct tcaagctctc ccgtaacgag atgatggtct ccgaggaaac
     3301 ccaggaggaa gtgatcgtcg gggtggacct ctttatcgag agctcgggac agccggagtt
     3361 catcgcaaaa aaatgccagc ggcatggggg tgtgaagttc aacctcgtca gtatcagcaa
     3421 caggggtacc caggtatggc ctaccggctc cgtgtatacg aatctcgtga atcagtacaa
     3481 tgtgcgtttc gagagcatcg acggcagccc cctgaaccag caggatgtat tgggattata
     3541 tgtcagcctg agcggcgact tccgcatctg ttcgtccgag ctgctgctca tgtggggggg
     3601 ccggaaggcc tattctctgg cgcagggtca ataaccgatc cgcgccgcct gcgcctcctc
     3661 cgtcgtccct gcccatgcgt cttctcctcc cccggcctcc tccgtcaccc ccgtctgctc
     3721 cgaacggtcg gccatcctca cccgcaactc agagtcgttt tgtggcggtg aagcccttgg
     3781 cgataagcca ctgggttatc ttatcccgct ggtcgccctg gatgatgatc tcgccgtcct
     3841 ttaccgaacc gccggtgccg caatgggtct ttaaccgccg gcccaggtcc tccagttccg
     3901 ccgcggcgcc gataaagcct tccacaaggg tgacggcctt gcctgcccgc tgccgggtat
     3961 ccagtcttac cttcagcttt tgctgtttgg ggagcaggct ctcctgcggc gcgctttctt
     4021 cctccaccct gaaatccgga tcggtggaat agacgatacc attgctgttg gctttcttct
     4081 tgctcatggg ttgtcgtttt tgtttgtcct tcggacaggg gccccgtggg ccatttttgg
     4141 tc
//
LOCUS       DATGFG010000235         5720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1080168, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000235 DATGFG010000000
VERSION     DATGFG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080168"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VKQ52_04340"
     CDS             <1..481
                     /locus_tag="VKQ52_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86443.1"
                     /translation="PAESILPISVGQVLANEAPSQNNTAPILAIRKDTIDHGKRILYL
                     HCTAPQGTNSVNLAFNPDDPVPGITVDGRAPSGSGPGAGEAARQPGYRWITYHGIPPE
                     GFDLVLELAPDKKYAGSLTARSLGIPLPQGAGSYPPGIIPSPGSISNTTLVTKKFSF"
     gene            542..2653
                     /locus_tag="VKQ52_04345"
     CDS             542..2653
                     /locus_tag="VKQ52_04345"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HLZ86444.1"
                     /translation="MRTLLFLAACLINTTLLFAGTPSRSATPNLARRPDLTATANLTA
                     TANLARRPNLARRPDLTATPNLAPPAAYDLKSPDGTLTVHISTTGRIQWSADQDGQPV
                     ILPSTLALHLETGETLGENVRILSAPLGQVNTSFAPINYKTSTITDQYTQLTLNCKGD
                     YSIVFRVYNDAVAYRFVTKRKGDLLIKNEDANFNFAADDKAFLPIQWDYRRGKIFNSS
                     FEALYHEIPLSQLPKDSLAFLPVLVDIGQGKKVAILEADLDDYPGMYLGLNETQKGLK
                     GVYAPYPLDAALASINYIPGNRADYIAKTTGSRSFPWRAIAISRQDKDLLNQDIVQKL
                     AAPPKLSDLSWIKPGQVAWDWWNNWNVTHVDFKAGINTETYKYYIDFAAANHLQYIIM
                     DEGWSDDLDLLQIKPVINLREIIDYGKQKGIGVILWATWYAVDHQMDTAFAKYSAMGV
                     KGFKIDFIDRDDQVAIASTFAIAQKAAQYKLLVDYHGTSKPAGLPRTWPNVIGYEGVK
                     GLENFKWTDEDQPRYVVTIPFIRMIAGPMDYTPGAMRNATQATFRPVNNNPMAKGTRC
                     QQLAEYIVFDAPLQMLSDNPTIYRKEQECTDFITGIPTTFDETVPLDGRVGEYLALAH
                     RKGTDWFVGAMTNWDPRDLTIDCSFLPEGTYESVVFKDGVNADRDATDYKKETLTIKR
                     GDKLTIHLAPGGGWVAKLRPQ"
     gene            complement(2753..3571)
                     /locus_tag="VKQ52_04350"
     CDS             complement(2753..3571)
                     /locus_tag="VKQ52_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLZ86445.1"
                     /translation="MLKSPMPVCFYSIALMVANPTAANPANSSVTESKNVLYTSGTKN
                     SHSQKDKIAFTLPDKKGLTFVSNYIRHNDECLTVVRERSEIPFTVIDSVLTRYHLPLE
                     LKYLAVIESELKPTAVSRVGAKGTWQLMRGTAHELGLKVNRQTDERKDLAKSTRAAAK
                     YLRDLHREFKDWLLVLAAYNGGPVPVYRAIHRAHSRNFWALQQYLPAESRLHVKKFIA
                     TTYYFEREAARPVLVAQGGPMPIAPETSDEKFSRLMRESALSLQASNELIAAGN"
     gene            4682..5146
                     /locus_tag="VKQ52_04355"
     CDS             4682..5146
                     /locus_tag="VKQ52_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86446.1"
                     /translation="MNYQIKELKTIFKDTYNSTGFDFNKADSLTIIYQTNNTSSLANY
                     IIISGKDTLRSTERLALCREIHNGGGKLEPLNKDAAMFKVYKTSNGNIPEPFLALAVK
                     SDTAYAFASDVDCPVDIGINSIIITAKKKNGRYKIGDYYLHEFSFVAITTKK"
     gene            5277..5447
                     /locus_tag="VKQ52_04360"
                     /pseudo
     CDS             5277..5447
                     /locus_tag="VKQ52_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1419 a         1772 c         1349 g         1180 t
ORIGIN      
        1 tccggcagaa tcgatcctcc ccatcagcgt cggccaggtc ctcgccaacg aagccccgtc
       61 acagaataat acggccccca tcctggccat ccgaaaagac accatcgatc acgggaaacg
      121 catactctac cttcactgca cggcgccgca gggcacaaac tccgtcaacc tggcctttaa
      181 cccggacgat cccgtgcccg gcataaccgt cgacggacgc gcaccctcgg gctccggacc
      241 cggcgcgggc gaggcagccc ggcaacctgg ctaccgctgg atcacctacc acggcattcc
      301 cccggaaggc ttcgacctcg tgctcgaact ggccccggac aaaaaatacg ccggctccct
      361 caccgcccgc agcctcggta tcccgctgcc ccagggcgcc ggcagctacc ctcccggtat
      421 catccccagt ccgggcagca tcagcaacac tacccttgta acaaaaaaat tctccttcta
      481 aacgcaggct tgcgccctcc ggcctatatt tgaacccgtc caccactaaa atttgcaaaa
      541 aatgagaacg ctgctcttcc tggccgcttg cctgatcaac accaccctcc tcttcgccgg
      601 tacccccagc cgatccgcca cccccaacct cgcccgccgc cccgacctca cggccactgc
      661 aaacctcacg gccactgcaa acctcgcccg ccgtcccaac ctcgcccgcc gccccgacct
      721 cacggccacc cccaacctcg cgcctcccgc cgcctacgac ctcaaatccc ccgacggcac
      781 cctcaccgtc catatctcca ccaccggccg gatccaatgg tcggccgacc aggacggcca
      841 gcccgtcatc ctcccctcga ccctcgccct ccacctcgag accggcgaga ccctcggcga
      901 aaacgtaaga atcctctccg cacccctcgg gcaggtgaac accagcttcg cccccatcaa
      961 ctacaaaaca tccaccatca ccgaccaata cacccagctc accctcaact gcaaaggcga
     1021 ctacagcatc gtctttcgcg tatacaacga cgccgtcgcc taccggttcg ttacaaaaag
     1081 aaaaggcgac ctcctcataa agaacgaaga cgccaacttc aacttcgccg ccgacgacaa
     1141 ggccttcctc cccatccaat gggactaccg ccgcggcaag atcttcaact cctccttcga
     1201 agccctttac cacgagatac ctctttcaca gctgccaaaa gactccctgg ccttcctgcc
     1261 ggtactcgtc gacatcggtc aggggaagaa ggtcgccatc ctcgaagccg atctcgatga
     1321 ctatcccggg atgtacctcg gcctcaacga gacccaaaag ggcctgaaag gcgtctacgc
     1381 accctatccc ctcgacgccg cgcttgccag catcaactac atccccggta accgcgccga
     1441 ttatatcgcg aaaaccaccg gcagccgcag cttcccctgg cgcgccatcg ccatcagccg
     1501 ccaggacaag gatctcctca accaggacat cgtccagaaa ctggccgcgc cgccaaaact
     1561 cagcgatctc agctggatca aacccgggca ggtggcctgg gactggtgga acaactggaa
     1621 cgtcacccac gtcgacttca aggccggtat caataccgaa acctataaat actatatcga
     1681 cttcgccgcc gccaaccacc tccaatacat catcatggac gagggctggt cagatgacct
     1741 cgatctgctg cagatcaaac ccgtcatcaa cctccgggag atcatcgact acggcaaaca
     1801 gaaaggcatc ggcgtcatcc tctgggccac ctggtacgcc gtcgaccacc agatggatac
     1861 cgcctttgcg aagtattcgg ccatgggcgt caagggattc aagatagact tcatcgatcg
     1921 cgatgaccag gtcgccatcg cctccacctt tgcgatcgcg caaaaggccg cgcaatataa
     1981 attattggtc gactaccatg gaacatcgaa acccgccggg ctgccgcgca cctggcccaa
     2041 cgtcatcggg tatgaaggag taaaaggatt ggagaatttc aaatggacgg atgaggacca
     2101 gccccgctat gtcgtgacga ttccctttat ccggatgatc gccggaccga tggattacac
     2161 acccggcgcc atgcgcaacg ccacacaggc cactttccgg ccggtgaata ataaccccat
     2221 ggccaagggc acccgctgcc agcaactggc tgaatacatc gtcttcgacg cgcccctgca
     2281 gatgctctcg gacaacccga ccatttatag gaaggagcag gaatgcacgg atttcatcac
     2341 gggtatccca accacattcg atgagaccgt tcccctcgat ggaagggtag gggagtacct
     2401 cgcactcgcc caccgcaagg gaaccgattg gttcgtcggc gcgatgacca actgggaccc
     2461 ccgcgacctg accatcgact gctccttctt acccgaaggg acatacgagt ccgtcgtctt
     2521 caaagatggc gtcaacgccg accgcgacgc caccgactat aagaaggaga ccctcaccat
     2581 taaacgcggc gacaagctga ccatccacct cgcccccggc gggggatggg tggcgaagct
     2641 ccggccgcaa taatagcctc cggcgggccg ccccaactgc cctggccaac atccgacccg
     2701 cccaactcac ggggcccaca tccgggccga cccgactcac ggggcctaca tcctaattcc
     2761 cggcggcgat caattcattg gacgcctgca acgatagcgc agactctctc atgagccggc
     2821 tgaacttctc atccgacgtc tccggcgcga tcggcatcgg gcccccctgc gcaaccagga
     2881 caggccgcgc ggcctcccgt tcgaaataat aggtggtggc gatgaatttc ttcacatgca
     2941 gcctcgactc ggcgggcagg tactgttgca gcgcccagaa attccggcta tgggcccggt
     3001 ggatggcgcg atagacaggc accggaccac cgttataggc ggccagcacc agcaaccagt
     3061 ccttgaactc ccggtgcaga tcacgcaggt atttggcggc agcccgggtg ctcttggcga
     3121 ggtccttacg ctcgtccgtc tggcggttca ccttcagacc cagctcatgc gcagtgcccc
     3181 gcatcaactg ccaggtcccc ttggcgccca cccgcgacac ggccgtaggc ttcagctccg
     3241 actcgatcac ggccagatac ttcagctcca atggcagatg ataacgcgtc agaacggaat
     3301 cgatcaccgt gaacgggatc tcgctccgct ccctgacgac cgtcaggcac tcgtcgttgt
     3361 ggcggatata attactgacg aatgtcaacc ctttcttatc gggaagggta aaggcgatct
     3421 tgtccttttg tgaatgggaa tttttcgtgc cactggtata aagcacgttc ttggactccg
     3481 taacggagct gtttgcagga tttgccgcgg tagggtttgc taccatcagg gcaatactgt
     3541 aaaaacacac aggcattggc gacttcaaca tcgttttagt ttttgggtaa aacaatgccg
     3601 gccccaggaa tggatcaatt tggaaggtaa aacatagaat attggcgaga gtcgtagtct
     3661 gtccgtgggt acggttcatt agggtttttc atttgcggaa gactacaacc ggatatgcaa
     3721 atatagccac tccggagggg aatgtggaag gggttctact acggagaaat acgatccgct
     3781 ttcaatgcct tgtttctcaa tactgttaca atctaaagac ttatgcagat ctcaataatt
     3841 ggctgatttc taaggaaaac ctggataagc aaacctcatc gggcgttccc ctccgccgcg
     3901 ccggctatcg ccggggctcg tccctcgccc ctcgcttcgc tcggccgcgg cggcgtcagg
     3961 tcgggctctt cgctgtagtc ctcggctttc agcctccggg ctgccgctgc gatccctaac
     4021 gtggaacccg aacaatggcg taaggcccct gcacgatttc cttctgctca gggtcgttcc
     4081 accttaacta tcggctcgct tctccgcatg ccgtccgaga acttcgtcgg agctgctccg
     4141 acacgctcct tctcgttcgc aatcttcgct tcgctcgatt gtcttcttat cgcgtcggcg
     4201 ccttggggtc gtgctgcaac attgatccgt taatccgcgg agccctggca gcggcggcgc
     4261 aattccgccc gaccgcaagg acgaggccgc ttatgcaaag tagggacagc cggctggccc
     4321 tggtggggcg ggtgcacagg taaaggttac tctccaccgc cgctgtggac cattcattcg
     4381 ccgcgcggtc ttcctactag aagggaacga aaggcataaa agacggcaaa caaattttta
     4441 gaccagaata acatacgtac ttttaagcat tagctgaaat tatatggcca ggcatttgat
     4501 aataataatc ttatcgcttt tagtaagacc tggttttagc cagtctttta cagattcata
     4561 caggctatac tgtaaaaata aaatcaattc ttccatacga caacgagtta ttcggtcatc
     4621 tgacactcgt gacaattctg ttgaaataag aaatagctgg tatacgcaat taaatgaaag
     4681 attgaattat cagataaaag aacttaaaac aattttcaag gatacttata atagcacggg
     4741 gttcgacttc aacaaggcag attcattgac tattatctat cagaccaata atacgtccag
     4801 tttagcgaat tacattataa tctccggcaa agacaccctg agatcaactg aaagattagc
     4861 cttatgccgg gagatacata atggtggcgg caagttagag cctttgaaca aggacgctgc
     4921 aatgtttaaa gtttacaaaa catccaatgg aaatattcct gagccgttcc tggcacttgc
     4981 agtaaagtca gacacggctt atgcctttgc gtcagacgtc gattgtcctg ttgacatcgg
     5041 aattaattca ataataataa cggcaaaaaa aaagaatggc cggtataaaa taggggatta
     5101 ttacctacat gaatttagtt ttgtcgcaat tactacaaag aaatagactc tcacggcaac
     5161 gtagagtgta aaatgaactg tgtttaaagt taaattctta actttaaaca tagtaaaaat
     5221 gaaaaagaaa ttcccaccac caacagaacc attcgattat gaattagcag tacgcgctgg
     5281 tgattaattc ctgggagaac aactggccga agctatcgac ctattttaaa tacccggccg
     5341 gtattcgtaa gttgatttat acgaccaata cgattgaagg ctatcatcgc cagatccgga
     5401 aggtcactaa aaacaaaggg gcttttacca atgacatggc tctgttgtaa ctggtatacc
     5461 tggctaccga gcgggtggcc aggaaatgga cgatgccatt acagggatgg gctgtgtcag
     5521 catcgcagtt aaagattata tttggggaac gaatgaaaac agatttgtga tactgcaccg
     5581 gtagaaaaga ggcggcgcct tgccctttgc atagagccgt tgccgcttct tttaaagaaa
     5641 atcgaaacgc tcaacttaat caaatgctta aacaagcttg tattattata tttattttat
     5701 catcggggtg caacgcttgt
//
LOCUS       DATGFG010000236         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_383904, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000236 DATGFG010000000
VERSION     DATGFG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383904"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VKQ52_04365"
     CDS             <1..335
                     /locus_tag="VKQ52_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86447.1"
                     /translation="SYMDEAALCDRVALLQNGHILSVDSPEGIRRGFGRELWAVKAKE
                     SFRLLNQLREEPAVESCYPFGRYHHVVLRKGGRAELEDLLRQGEYTEAEVAPAKPGIE
                     DCFIALMK"
     gene            complement(316..1086)
                     /locus_tag="VKQ52_04370"
     CDS             complement(316..1086)
                     /locus_tag="VKQ52_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLZ86448.1"
                     /translation="MQHVGIALSGGGARAIAHLGVLRALEERGIRPTAIAGASAGALI
                     AALYATGLPPEEIKDIFHDTAYFGISHLLLGHPGLFDMDGMRSALEKHLAGRNFEHLP
                     IRLFVAATNLTAGRAEIISTGPVIEPLLASACVPVIFEPVVYRGCEWVDGGVLNNFPV
                     EALIPHCDYIIGSHVNRLDSPMPPARSLHKSHILERCFHLAIAGSVYEKAAQCDVFLD
                     HPDLADFGMFDSKEADHLFDLGYEYGKRALSAPISSAR"
     gene            1215..2264
                     /locus_tag="VKQ52_04375"
     CDS             1215..2264
                     /locus_tag="VKQ52_04375"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86449.1"
                     /translation="MNISEISQKVLLPSPEVRKPSVAIVLPYNPKMSPRHEIETQLRR
                     VLGMAEKRLLDAHPAETAMPVVRRLQQLARKLNSATLKVSIALFVSEDVARVTYMDFP
                     VEERVMVDEPFLVRDIADCRPSTREYLVLLLSAKESKMYLESGNGLKLIKSNAPQSVY
                     AYLNEVPERTGNFSDAADRREVMLNKFLHHMDEGLGAVLKVYPLPVFVVGPERVAGHF
                     ARVTRNDRQIAGYVYKHAIDASEAELQQWLEPLLADWRQTKQQLLLRQMEKAAEAGKL
                     VCGVHEARKAAACKNSRLIVVDRDGHQGEEADFYSGGEIDVIIEKVLESGGEIEKMDH
                     ELLKPYGPIALIRYY"
     gene            2309..3859
                     /locus_tag="VKQ52_04380"
     CDS             2309..3859
                     /locus_tag="VKQ52_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HLZ86450.1"
                     /translation="MFKEKSGHILLFCSIVTLCTAVLQYLNPQYSIIDGGLIGAVLLT
                     IFLNRDVYTRLFGGISVLLIVLASFYQHDNMDRQQLILQHAFSLLIVIMATLFVLYVK
                     RLYRNIESDQHQVTALFEHATEGIILTDDQGKIVLLNPAAFRLFEYDQDELPGQSIDV
                     LIPQRLHSAHVKYREGFYHHPGNRTMGHGRDLFARKKDGTEFPVEVSLSYYHQKQKLY
                     VIAFIVDITQRKQSELELVHRQQQLQQITEDVRRLNAGLEAKVEQRTLILREALQELE
                     RSQQELSEALSKEKELNEIKSRFVSMASHEFRTPLSTVLSSAALLSRYTRTEEQGNRD
                     RHIKRIKDAVKHLNDLLEDFLSLGKLEEGKIKAEPAEFAVREFLEEVVEEMQPLAKKD
                     QQIVSECDGLEVLVSDKRLLKNILINLLSNAVKFSPEGAKIVLCVERTAAGFLRLSVK
                     DEGIGISAEDQQHLFSSFFRGGNAQNIEGTGLGLHIVRRYAELLGGTIGLKSVLGAGT
                     TVTVELPG"
     gene            complement(3912..>4212)
                     /locus_tag="VKQ52_04385"
     CDS             complement(3912..>4212)
                     /locus_tag="VKQ52_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HLZ86451.1"
                     /translation="LPIIAMPSVTSTGISRIVPVLKEGAGVVTTRGHVHWVATEYGRV
                     NLFGKNLKQRAQLLINIAHPAHREWLDRAFYQRYRHTTTAVAAHPEPKPEPAAGL"
BASE COUNT          921 a         1032 c         1416 g          843 t
ORIGIN      
        1 cctcctatat ggacgaggcc gcgctttgcg accgggtggc cctgctgcaa aacgggcata
       61 tcctgagtgt ggattcgccg gaaggcatcc gccggggttt tggccgggag ttatgggcgg
      121 taaaggcgaa ggaaagtttc cggctgttga accagctgag ggaagagccc gctgtcgaga
      181 gctgttatcc ttttggccgg tatcatcatg tggtgctgcg aaagggggga agggccgagt
      241 tggaggacct cctccggcag ggcgagtata cggaagcgga ggtggcgccg gcgaagccgg
      301 ggatcgagga ctgctttatc gcgctgatga aataggggcc gataacgcgc gtttgccata
      361 ttcatagccg aggtcgaaaa gatggtcggc ctctttactg tcgaacatgc cgaagtccgc
      421 cagatcggga tggtcaagga agacatcgca ctgcgcggct ttttcgtata cggatccggc
      481 gatggcgagg tggaagcatc gctcgaggat atggctctta tgcagggacc gggcgggcgg
      541 catggggctg tcgagccggt tgacatggga gccgatgata tagtcgcaat gggggatcag
      601 cgcttctacg gggaaattgt tgagcacgcc gccatccacc cattcgcagc cccggtaaac
      661 gacgggttcg aagatcacgg gcacgcaggc ggaggccagc aggggttcga tgaccgggcc
      721 ggtggagatg atctctgccc gtcccgcggt gaggttggta gcggcgacaa agagcctgat
      781 gggcaggtgt tcaaaattcc tgccggccag gtgcttttcc agggcggatc gcatgccgtc
      841 catgtcaaag aggcccgggt ggccgaggag caggtgggag atgccgaaat aggccgtgtc
      901 atggaagatg tcttttatct cttccggcgg caggccggtt gcatagaggg cggcgatgag
      961 ggctcccgcg ctggccccgg ctatcgcggt gggacgaatt ccccgttctt cgagggcgcg
     1021 gaggaccccc aggtgagcaa tggcgcgggc cccgccgccg gaaagtgcta tgcctacatg
     1081 ctgcatgttc tttttttggt gaaggtaagc aggcaccggg gggcggccaa tgacgatgat
     1141 cattccccgc cgtgaccagc ctcatgtgag atggggggag cagctggata tctttgaatt
     1201 ctaaataact aactatgaat atttccgaga taagccaaaa ggtgctgcta ccgtcgcccg
     1261 aggtacggaa gcccagcgta gcgatcgtac ttccctacaa tccgaagatg agtccaaggc
     1321 atgagatcga gacgcaactg cgcagggtgc tggggatggc cgagaaacga ctgctggatg
     1381 cacatccggc tgaaacggcc atgcccgtcg tccggcggct ccagcaactg gcacgcaagc
     1441 tcaacagcgc tacgcttaag gtaagtattg cgcttttcgt ttccgaggac gtggccaggg
     1501 tcacgtatat ggatttcccg gtggaagagc gggttatggt ggatgagccg ttccttgtcc
     1561 gggacattgc cgactgcagg ccctcgaccc gggaatacct ggtcttgctg ctgagtgcaa
     1621 aggaatcgaa gatgtacctg gagagcggga acggattgaa actgatcaag agcaatgcac
     1681 cgcagagcgt ctatgcctac ctgaacgagg ttcctgaaag aaccggcaat ttctccgatg
     1741 ccgccgaccg aagggaggtg atgctgaaca aattcctcca tcatatggac gaggggctcg
     1801 gcgccgtcct gaaggtgtat cccttaccgg tgttcgtcgt cggtcccgaa cgggtggcgg
     1861 ggcattttgc ccgggttacg cggaatgacc ggcagatcgc gggttatgtg tataagcatg
     1921 cgatagatgc ttccgaggcg gagttacaac aatggctgga acccctgctg gccgactggc
     1981 ggcagacgaa gcagcagttg ctgttacggc agatggagaa ggcggcggag gcaggtaagc
     2041 tggtctgtgg cgtccatgaa gcccggaagg ccgcggcctg caagaattcg cggttgatcg
     2101 tcgtggacag ggatgggcac cagggcgaag aagccgattt ttactccggc ggagagatcg
     2161 atgtcatcat cgagaaggtg ctggaaagcg gaggggagat cgagaaaatg gaccatgagc
     2221 tgctgaaacc gtatggcccg atagcgttga tccgatatta ttgaaaggag agtcgtacat
     2281 ttagcaacaa aaaaaatcgg ccagcctgat gttcaaagaa aagtccggtc atatcctgtt
     2341 gttttgttcc attgtcactc tctgtacagc tgtattgcag tatctgaatc cgcaatacag
     2401 tattatcgac ggcggattga tcggcgccgt cctgctgacc attttcctga acagggatgt
     2461 ctatacgcga ctattcggcg ggatcagcgt gttgctgatc gtgctggcct ccttctacca
     2521 gcatgacaat atggatcgcc agcagctgat cctgcagcat gccttctcgc tgctgatcgt
     2581 gatcatggcc acgcttttcg tcctgtatgt aaaaagactt taccggaata tcgaatcgga
     2641 tcagcaccag gtgaccgcgc tgttcgagca cgccaccgag gggatcatcc tgaccgacga
     2701 ccaggggaag atcgtgctgc tgaatccggc cgctttccgg ctattcgagt atgaccagga
     2761 cgagctgccg gggcagtcga tcgacgtact gatcccccag cggcttcatt ccgcgcacgt
     2821 aaaataccgg gagggttttt atcatcatcc gggaaaccgg acgatggggc atggccggga
     2881 cctgttcgcc cggaagaagg acggcacgga gttccccgtg gaagtgagcc tgagctatta
     2941 tcatcagaag cagaaactat atgtgatcgc ctttatcgtg gacatcacgc agcgaaagca
     3001 atcggagctg gaactcgtcc accgccagca gcagctgcaa caaattaccg aagatgtgcg
     3061 gcggctgaac gcgggtctgg aggcaaaggt ggaacagcga acgctgatcc tccgggaggc
     3121 gctgcaggag ctggagcgaa gccagcagga gctgagcgag gcgctcagca aggaaaagga
     3181 gctgaatgag atcaaatccc gcttcgtatc gatggcttcg catgagttcc ggacaccgtt
     3241 aagcacggtg ttgtcctcgg cggcgttgtt gtcgcggtat acccggacgg aggaacaggg
     3301 caaccgggac aggcatatca aacggatcaa ggatgcagtg aaacatctca acgacctgct
     3361 cgaggatttc ctctcgcttg gaaaactgga agagggcaag atcaaggccg agccggcgga
     3421 gttcgccgtc agggagttcc tggaggaggt ggtggaggaa atgcagccgt tggcgaagaa
     3481 ggaccagcag atcgtgtcgg agtgtgatgg attggaggtt ctcgtaagcg acaaacggtt
     3541 actaaagaat attttgatca acctgctcag caatgccgtg aaattctctc ccgaaggggc
     3601 gaagatcgtc ctgtgcgtgg agcgaacggc ggccggcttc ctgcgtctct cggtgaagga
     3661 tgagggtatc gggatctcgg cggaggatca gcagcacctg ttcagcagtt ttttccgcgg
     3721 ggggaacgcg cagaacatcg aagggaccgg cctggggctg catatcgtaa ggcggtatgc
     3781 cgagttgttg ggcgggacta tcgggctgaa gagcgtgctt ggcgcgggga cgacggtgac
     3841 ggtcgaactg ccgggctgat aaaaagataa agaaatggaa gagcgccggg aggtcccggc
     3901 gcttttttat tttataaccc cgccgcgggt tcgggtttag gttcgggatg cgcggcgacg
     3961 gcggttgtgg tatggcggta gcgttggtag aaggcgcggt ccagccattc gcggtgggca
     4021 ggatgggcga tattgatcag gagctgggcg cgttgtttga ggttcttgcc gaagaggttg
     4081 acgcgaccgt attcggtagc cacccagtgg acatgaccgc gggtggtcac gacgccggcg
     4141 ccttccttca ggacgggaac gatccgggag ataccggtgg aggtgacgga gggcatggcg
     4201 atgatgggca gg
//
LOCUS       DATGFG010000237         7026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_383837, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000237 DATGFG010000000
VERSION     DATGFG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383837"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..729)
                     /gene="nusG"
                     /locus_tag="VKQ52_04390"
     CDS             complement(145..729)
                     /gene="nusG"
                     /locus_tag="VKQ52_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HLZ86452.1"
                     /translation="MTETVNQQETKTQPETKWYVLRVVSGKERKVKEYLDKEISRGGW
                     SEIVKQVFLPVEKVYKVQNGKKVMRERNYYPGYVMVEVTEGKLGDDLKEVIKNTNSVI
                     HFLGKENPIALRKAEVNKMLGKMDEMAESGSMSMSEPFIIGETIKIIEGPFNDFNGII
                     EEVNDEKKKLKVTVKIFGRSTPVELNYMQVEKIA"
     gene            complement(776..973)
                     /gene="secE"
                     /locus_tag="VKQ52_04395"
     CDS             complement(776..973)
                     /gene="secE"
                     /locus_tag="VKQ52_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HLZ86453.1"
                     /translation="MNKITTYFRESYRELLEKVTWPTWSQLQQSTVIVLVATLLVTLI
                     VWLMDMISSSILHFVYSLFGG"
     gene            complement(1032..1102)
                     /locus_tag="VKQ52_04400"
     tRNA            complement(1032..1102)
                     /locus_tag="VKQ52_04400"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1068..1070),aa:Trp,seq:cca)
     gene            complement(1190..2377)
                     /gene="tuf"
                     /locus_tag="VKQ52_04405"
     CDS             complement(1190..2377)
                     /gene="tuf"
                     /locus_tag="VKQ52_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HLZ86454.1"
                     /translation="MSKETFKRDKPHVNVGTIGHVDHGKTTLTAAITTILSSRGLAEA
                     KKYDEIDAAPEEKERGITINTAHVEYATANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTKEHILLARQVGVPRIVVFMNKVDLVDDPELLELVEMEIRELLSS
                     YGFDGDNTPIIQGSATGALAGEDKWVKKIDELMAAVDSYIPLPPRPVDQPFLMSVEDV
                     FSITGRGTVATGRIERGRIKVGEGIEIVGLQDAPLTSTVTGVEMFRKLLDQGEAGDNA
                     GLLLRGIEKTQIRRGMVLCKPGSITPHTEFKGEVYVLSKEEGGRHTPFFNKYRPQFYF
                     RTTDVTGEVTLPAGTEMVMPGDNTSLSVVLIQPIAMEKGLKFAIREGGRTVGAGQVTE
                     IVK"
     gene            complement(2499..2570)
                     /locus_tag="VKQ52_04410"
     tRNA            complement(2499..2570)
                     /locus_tag="VKQ52_04410"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2536..2538),aa:Thr,seq:ggt)
     gene            complement(2820..2895)
                     /locus_tag="VKQ52_04415"
     tRNA            complement(2820..2895)
                     /locus_tag="VKQ52_04415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2860..2862),aa:Gly,seq:tcc)
     gene            complement(2912..2997)
                     /locus_tag="VKQ52_04420"
     tRNA            complement(2912..2997)
                     /locus_tag="VKQ52_04420"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2961..2963),aa:Tyr,seq:gta)
     gene            complement(3125..3197)
                     /locus_tag="VKQ52_04425"
     tRNA            complement(3125..3197)
                     /locus_tag="VKQ52_04425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3162..3164),aa:Thr,seq:tgt)
     gene            complement(3418..3717)
                     /locus_tag="VKQ52_04430"
     CDS             complement(3418..3717)
                     /locus_tag="VKQ52_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HLZ86455.1"
                     /translation="MNVNIQTVHFDADGKLVEYVNKKMTKLNTFHDRIIKVDVFLKLD
                     NVVHTIKDKIAEIRVHVPRHHFFVKCSSKSFEESFDSAFESLVNQVKRKKDKELA"
     gene            complement(3913..4803)
                     /locus_tag="VKQ52_04435"
     CDS             complement(3913..4803)
                     /locus_tag="VKQ52_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLZ86456.1"
                     /translation="MDALIHSFIDYLTFEKRYSAHTIRSYGDDLEGFFVFLRDDFGGM
                     GLREAGPPVVRSWLAALKDGGLSSRSVNRKISSLRSFYKYQLRVGGVEQSPMAAIIAP
                     KVSKRLPVFVAQGDMAELFTEVRFPEDWEGLTDRLILAILYHTGMRLSELVQLRERQA
                     DVGNGTFKVLGKGNKERVIPVSPVLMAMVEEYRRLKRVQWEAPEVEFLLVGKKGKKLY
                     AKYVYRAVRGYLAQVTTIDKKSPHVLRHTFATHLMNAGAELNSVKELLGHASLAATQV
                     YTHNTIEKLKDIYKKAHPKA"
     gene            complement(4936..5130)
                     /gene="rpsU"
                     /locus_tag="VKQ52_04440"
     CDS             complement(4936..5130)
                     /gene="rpsU"
                     /locus_tag="VKQ52_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HLZ86457.1"
                     /translation="MLIIDSKDCENIDKALKKYKKKFEKAKVLVQLRERQSYTKPSVR
                     RRGEVLKAIYKQQVAAGKYE"
     gene            5289..5894
                     /locus_tag="VKQ52_04445"
     CDS             5289..5894
                     /locus_tag="VKQ52_04445"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HLZ86458.1"
                     /translation="MFTGIIEALGRIHSVETHGTNKTFWIESPISPELKVDQSISHNG
                     VCLTVEEVGGNSHRVTAIEETLRKSNLESWEPGHLVNLERCLIMNGRLDGHIVQGHVD
                     ATATCIDRRNANGSWEFRFEFPKKFSHLVIEKGSISLNGISLTIFNVKRSKFDIAIIP
                     YTFEHTNIQTVIPGSTVNLEFDIIGKYVSRITQTGETKKHE"
     gene            5891..6955
                     /locus_tag="VKQ52_04450"
     CDS             5891..6955
                     /locus_tag="VKQ52_04450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ86459.1"
                     /translation="MNKTPSVALVILNWNTSHYLKRFLPSLLATTYPNKHIYVIDNNS
                     TDDSIPLLKEQFPSVHILAMNENKGFAGGYNFGLKQIDTDYYLIVNSDIEVTPGFIEP
                     LVQLLENNPRIGICQPKLRALDHRDMFEYAGAAGGWIDQLGLPFTRGRVLTTIEKDEG
                     QYDATEELFWATGACMCFSAKLFESTGGFYDYYFMHQEDIDLCWRVRNMGYTIYACPQ
                     SVVYHKGGGTLSWENYLKTFFTFRNNYILLSRNLPFFHAAIVVSIRLVTDFFGWFYFL
                     GRHEPGVSKAMLKATLSYFKWLILRPGPRNHQPKGWKNKTGIYKGTILIPYFLRNKRK
                     FTELVRTNTAPHAQRALKEI"
BASE COUNT         1758 a         2230 c         1391 g         1647 t
ORIGIN      
        1 ctgctccttc gacatttgat gacgctcccg cgactcatca tttcgatacc gacaatgaca
       61 atggcaattt ctagacaaag agcccaacat aagaaaagcc ttccatccgg aaggcttttt
      121 tattattaac atcttaagtg acgactaagc gatcttctcc acctgcatat agttcaactc
      181 caccggcgtg ctccggccaa agatcttcac cgtcaccttc agcttcttct tctcatcatt
      241 cacttcttcg atgatgccgt tgaaatcatt gaacggccct tcgatgatct tgatcgtctc
      301 accgatgatg aacggctcac tcatgctcat actgcccgac tcggccatct catccatctt
      361 cccaagcatc ttattcactt ccgccttccg caacgcgatc gggttctcct ttccaaggaa
      421 atgaataaca ctattggtat tcttgatcac ttctttcaga tcatctccaa gcttcccctc
      481 cgtcacctcc accatcacat accccgggta gtagttacgc tcacgcatca ccttcttccc
      541 gttctgaacc ttatatacct tctctacggg caggaacacc tgcttcacga tctcactcca
      601 cccgccgcgg gagatctctt tatcgagata ctcttttacc ttccgctctt tcccgctgac
      661 gacccgcagg acataccact tggtctccgg ctgggtcttt gtttcctgtt gattgactgt
      721 ttccgtcata aaatgaattt tggcaagata tttaccggtg ccaaactcac gcgatttagc
      781 ctccgaataa agaatagaca aagtgcagga tcgaactgga tatcatatcc atgagccaga
      841 cgatcagcgt gaccagcaat gtggcaacca gcacgatcac cgtggactgc tgcagttgag
      901 accaggtagg ccaggtcacc ttctccagca actccctgta cgactccctg aaataggtcg
      961 ttattttgtt cattgttgtt gtttttctac cgccgtccgc atcaaccccc ggctgtgcga
     1021 cccggcttct gcacgggcac taggattcga acccagactg aaggttttgg agacctctgt
     1081 actaccgtta tactatgccc gtagaagcta agagctatcc cgaacaaatt cagaacagct
     1141 cttagtctta caaagatata aaaaatcctc cgaaattaac tttcggcgct tactttacga
     1201 tttcagtcac ctgacctgca ccaaccgtac ggccaccttc gcggatagcg aatttcagcc
     1261 ccttctccat ggcgatcggc tggatcagca caacgctcag ggaagtgttg tcaccaggca
     1321 ttaccatctc ggtaccggcc ggcagcgtaa cctcaccggt tacgtccgtt gtacggaaat
     1381 agaactgggg acgatacttg ttgaagaacg gcgtgtgacg gccaccctct tccttgctca
     1441 gaacgtaaac ttcgcccttg aactcggtat gcggagtgat ggaaccgggt ttgcaaagaa
     1501 ccatgccacg acggatctgg gtcttttcaa taccacggag cagcagcccg gcattgtcac
     1561 cggcttcacc ctgatccagc agcttacgga acatttcaac acccgttacc gtagaggtca
     1621 gcggagcatc ctgcagacca acgatctcga taccttcacc caccttgata cggccacgct
     1681 cgatacgacc ggtagcaacc gtaccacgac ccgtgatgga gaacacgtct tcgacagaca
     1741 tcaggaaggg ctgatcgaca ggacggggag gcagcggaat atagctgtcg acagccgcca
     1801 tcagctcgtc gatcttcttt acccacttgt cttcaccagc cagtgcgccg gtagcgctac
     1861 cctggatgat cggcgtattg tcgccgtcaa aaccatagga agacagcagc tcacggattt
     1921 ccatctccac cagttccagc agctcggggt catcaacaag gtcaaccttg ttcatgaaca
     1981 caacgatacg gggaacacct acctgacgcg caagcaggat gtgctctttc gtctggggca
     2041 tcggaccgtc agtggcagca actaccagga tagcgccatc catctgagca gcaccggtga
     2101 tcatattttt tacatagtca gcgtgaccag gacagtcaac gtgagcatag tgacggttag
     2161 ccgtcgcata ttcaacgtgt gctgtgttga tggtaatacc cctctccttt tcttccggcg
     2221 cagcgtcgat ctcgtcatat ttttttgctt ccgccaaacc tctcgaagaa aggatggtag
     2281 taatagcagc agtcaaagtg gtcttaccat ggtcaacgtg accaatcgta ccaacgttaa
     2341 cgtggggttt gtccctcttg aaggtctctt ttgacattgt tatctgtttt taagttgtgt
     2401 tttaaaatct tgacaggtcc cccacccccc ttagggcgtt gaaacctgtt ctattgctga
     2461 caaatcaatt cttccgttca aaacctgcca aaaaaaagag ccgttgatga gaattgaact
     2521 cacgacctct tccttaccaa ggaagtgctc tacccctgag ctacaacggc tggttcattt
     2581 cttccaccgc cccgcggcca gcccgcatcc agcccatcac gcacttccaa acgcctcccg
     2641 cacatcccat caggcacccg ggccgcctcc ccttcgaaca catcccctca ataaccccgg
     2701 ctcaaccaag tccttcagca tgcgtcccgg cccttaccct cttctgcctc acccgctccg
     2761 cggaccttat cgcccacccc ttctcaaccg gaagacccgc cggctatccc ggcttttcat
     2821 ggagcggaag acgaggttcg aacccgcgac ctacagcttg gaaggctgtc gctctaccaa
     2881 ctgagctact tccgcatgaa agcgataaaa ctggtgggca aggatggatt cgaaccaccg
     2941 aactccgaag aggacagatt tacagtctgt tgccgttggc cacttggcta cttgcccggc
     3001 tatcaaaaat gtgcgggaga tgcacttcta acaaaaatca gcgcatctcc cgcaaagcgc
     3061 taatgtctgt cctttcggac aatccgcctc cggcggatgc ggtcgggagg ttctacctcc
     3121 ctagagccag agaagggact cgaacccccg accagctgat tacaaatcag ctgctctacc
     3181 aactgagcta ctctggcttg taactcaccc caaacgctta tcacactgat ctactgcttt
     3241 ttttgggaag gcaaaggtag gtaaaatcgt gaacttcaaa aattttcaac ccgttttttt
     3301 aagttattcc ttttcacgcc cccacatcgg cccccccaaa caaaaaaaag acccccaatg
     3361 gaggtctttt tacaattttt tactgatggt tgccgatgga ctgcaacatc catctatcta
     3421 tgcaagttct ttatcttttt ttctctttac ctggttaacc agcgactcga atgcgctgtc
     3481 aaaagattct tcaaatgatt tggatgaaca tttaacgaaa aaatggtgtc tgggaacatg
     3541 gaccctgatc tccgcgatct tgtccttgat ggtgtgaacc acattgtcca atttgaggaa
     3601 tacgtctact ttaatgatcc tgtcatgaaa cgtgtttagt tttgtcattt tcttgtttac
     3661 atactctact aacttaccgt ctgcatcaaa gtgcacagtt tgaatgttta cgttcataca
     3721 tcccaaattt taatcagtga acaataaatt aaagaactac tactgctgca ggataaggtc
     3781 tacttttcaa tacctgaagc cgcgcttcag tgacacaatg aagttaatct ttacaaaggg
     3841 tttttccaaa ttatccgggt ggttttaacg gcataacact gcagttttta acagatcacc
     3901 ttacccccgc cactacgcct tgggatgcgc tttcttatag atatctttca atttttcgat
     3961 ggtattatgc gtgtacacct gcgtagccgc cagactggca tgccccagta actccttcac
     4021 cgaattcaac tccgcccccg cattcatcag atgcgtcgca aacgtatgcc gcaacacatg
     4081 cggactcttc ttgtcgatcg tcgtcacctg cgcgagatac ccgcgcacag cccggtacac
     4141 atatttcgcg tataacttct tccccttctt ccccaccagc aggaattcca cctccggcgc
     4201 ctcccactgc acccgcttca accgcctgta ctcctccacc atcgccatca ataccgggct
     4261 caccggtatc acgcgctcct tattcccctt ccccaatacc ttgaacgtcc cattacccac
     4321 atccgcctgc cgctcccgca actgcaccaa ctcactgagc cgcatcccgg tatgataaag
     4381 gatcgccagt atcaaccggt ccgtcaatcc ctcccagtcc tcggggaacc gcacctcagt
     4441 aaaaagctcc gccatatccc cctgcgccac aaagacagga agccgcttgc tcaccttcgg
     4501 ggcaatgatc gccgccatcg gcgactgctc cacaccccca acccgcaact ggtacttgta
     4561 aaaacttcgc agagaagata ttttccggtt caccgaccgc gacgaaagcc ccccatcctt
     4621 caacgccgcc agccagctcc tcaccaccgg cggccccgcc tcccttagcc ccatcccccc
     4681 aaaatcgtcc cggagaaaga caaaaaaccc ctccaggtca tccccatacg accggatcgt
     4741 atgcgccgaa taacgcttct cgaatgtcaa ataatcaata aacgaatgga taagcgcgtc
     4801 caaacacaaa tcattttgtt tcactaagtt agtaaaaaaa aatggacgcc cggcaccaca
     4861 ccccgaaacc ggccccccta aaaacaaaaa aggctgtcca acggacagcc tcccgaatat
     4921 atttgtagga tcctactatt catacttgcc tgcggcaacc tgctgcttgt agatcgcctt
     4981 caacacttcc ccacgccggc gcacggacgg cttggtatac gactgacgct cacgcaattg
     5041 gaccaatacc ttggcctttt caaacttctt cttgtacttc ttcagcgctt tgtcgatgtt
     5101 ttcgcaatct ttggaatcga tgatcagcat aactttttat accccctttt ggttatcaaa
     5161 tcgaaaatgg actgcaaaaa taggaaaaat tccggaaaat cgaaactgtc cggcccaaaa
     5221 tctgatttcc ccctccctga ccgcgaaatt caagcgccat tcctctaaaa tcccgaaatt
     5281 tggcccctat gttcaccgga atcatcgaag cactcggcag gatccactcc gtcgaaaccc
     5341 atggcaccaa taagaccttc tggatcgaat cccccatttc ccccgaactg aaagtcgacc
     5401 aaagcatctc ccacaacggc gtctgcctca cggtcgaaga agtcggcggc aacagccacc
     5461 gggtgacggc catagaggaa accctccgca aatccaacct ggaatcctgg gaacccggcc
     5521 acctcgtcaa cctcgaacgc tgcctcatca tgaacggccg cctcgacggc catatcgtgc
     5581 agggccacgt cgacgccacc gccacctgca tcgaccgcag gaatgccaac ggctcctggg
     5641 aattccgctt cgaattccca aaaaaattca gccacctcgt catcgaaaaa ggctccatca
     5701 gcctcaacgg catcagcctc accatcttca acgtaaaaag atcgaagttc gacatcgcca
     5761 tcatccctta taccttcgaa cataccaata tccaaaccgt catcccggga agcaccgtca
     5821 acctggagtt cgacatcatc ggcaaatacg tttcccgcat cacgcagacc ggcgaaacca
     5881 aaaaacacga atgaataaaa ccccctctgt agcccttgtc atactcaact ggaacacttc
     5941 acactacctg aagcggttcc tgccctccct tctggccact acctacccga ataaacatat
     6001 ttacgtcatc gacaataact ccaccgatga ctccatcccc ctgctgaagg aacagttccc
     6061 ctccgtccac atcctcgcga tgaacgagaa caaagggttc gccggcggct ataacttcgg
     6121 cctcaaacag atcgataccg actattacct catcgtcaac tccgacatcg aagtaacacc
     6181 cggcttcatc gaacccctcg tccaactcct cgagaacaac ccacggatcg gcatctgcca
     6241 gccaaaactc cgcgccctcg accaccgcga catgttcgaa tacgccggcg ccgcaggcgg
     6301 atggatcgat cagctgggcc tccccttcac ccgcggccgt gtgctcacca ccatcgaaaa
     6361 agacgagggc cagtacgacg ccaccgaaga actcttctgg gccaccggcg cctgtatgtg
     6421 ctttagcgcc aaactcttcg aaagcaccgg cggcttctat gactactact ttatgcacca
     6481 ggaagacatc gacctctgct ggcgcgtccg caacatgggc tataccatct acgcctgccc
     6541 ccaatccgtc gtctatcaca agggcggcgg aaccctctca tgggagaatt atctcaagac
     6601 cttcttcacc ttccgcaata actatatcct cctctcccgc aacctgccct tcttccacgc
     6661 cgccatcgtc gtcagcatcc gcctcgtcac cgacttcttc ggctggttct acttcctcgg
     6721 ccgccacgaa cccggcgtca gcaaagcgat gctaaaagca accctctcct acttcaaatg
     6781 gctcatcctc cgcccgggcc ccagaaacca tcagccaaaa ggatggaaga ataaaacggg
     6841 catctataag ggcactattc taatccccta ttttcttaga aataaaagaa aatttacgga
     6901 acttgtccgg acaaacaccg caccgcatgc tcaacgcgct cttaaagaga tataatcatt
     6961 accggaaaga aaaatacttc cagtcccccc tcttccaggc gcgaaaacaa tatgccttcg
     7021 tcggct
//
LOCUS       DATGFG010000238         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_655375, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000238 DATGFG010000000
VERSION     DATGFG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655375"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VKQ52_04455"
     CDS             <1..1183
                     /locus_tag="VKQ52_04455"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HLZ86460.1"
                     /translation="NEEWMIQTSTTGKLIAQHQIERQRRLDPSRTCTYAANVGNVWHG
                     VNEVIPVRGFNYNLGGLDAYRKEHPDQPIMGTEVASTVSTRGIYVKDTVNAWVPDYDS
                     VAPSWASTAEYWWRIAADREWFMGGFAWTGFDYRGEPTPFQWPDINSHFGIMDMCGFP
                     KTVYYYYQSWWTDKDVLHIAPSWNRSGKEEGKPVAVWVNSNAESVELMLNGKSLGKKE
                     MPRNGHLEWMVPYTPGRLEAVGYKKGRKITTVVETTGEPYRIVVTPSKAALAADGRDA
                     MVYNCSVVDKQGREVPDAQNLLHFSVSGDAKVIGVGNGDPSSHEPDQYPEGGWQRHLF
                     NGRCQVIVLSAAGERGPGAAGAPVQSAAGGVRGAAGGNGVFFVTGDGLLPSQTPVPLG
                     H"
     gene            1218..2231
                     /locus_tag="VKQ52_04460"
     CDS             1218..2231
                     /locus_tag="VKQ52_04460"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ86461.1"
                     /translation="MAMSSEIKKQIAVALYSSLAVSVLTTTPVFMMTRISFSLYPWTL
                     LFATMMTVMMWGLNILLFYFSGGRRVRRYIWSYVVCLTFALGMFHGLLNMPMDTGIGS
                     GGRGFYFHIILFFSVDTVLLIVQDLVVVRERKAVVELENAQLRMRNMEAANLLLQQQV
                     QPHFLFNSLSTLKSLIKYSPADAEEYVVKLSGFLRSSMASHASKLVTVEQELGLCTDY
                     LEMQLIRFGEALRYSVEVGEEERRGFLPVFALQLLIENAIKHNVLTKERPLAIRVEGM
                     GKMVRVVNNLQLREGVEAGGTGLTNLRERYRVLGGGEVVIATSETEFSVSISLLSHDD
                     RDH"
     gene            2212..>2766
                     /locus_tag="VKQ52_04465"
     CDS             2212..>2766
                     /locus_tag="VKQ52_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ86462.1"
                     /translation="MTIVIIEDEPLTARDLAACVRVAEPTVEIVASLASVGEAKDWFD
                     RHAAPDLIFSDIQLGDGQAFVVFERLMQMVPVIFCTAYDEYALEAFRAAGIDYILKPF
                     TEKSVGAALAKYKALRGADQTGMEVAGGKMAEPAAAGRKMVGPVAAGGKMAGPAGAIL
                     VFYKEKILPVKVGDIAFFYLEKEVV"
BASE COUNT          520 a          571 c         1052 g          623 t
ORIGIN      
        1 gaacgaggaa tggatgatcc agacctcgac tacgggaaag ctgatcgcgc agcaccagat
       61 cgagcggcag cggcggctcg acccgagccg gacctgtacg tatgcggcca atgtggggaa
      121 tgtatggcat ggggtgaacg aggtgatccc ggtgcggggg ttcaactata atcttggcgg
      181 actcgacgcg taccgcaagg aacaccccga tcagccgatc atgggtacgg aggtggcgag
      241 tacggtgtcg acgcggggta tttatgtgaa ggatacggtc aatgcctggg ttccggatta
      301 tgacagtgtc gcgccgtcgt gggcgagcac ggccgagtat tggtggcgga tcgccgccga
      361 ccgggaatgg ttcatgggtg gttttgcctg gacgggattc gattaccgtg gcgagcccac
      421 gccttttcag tggccggata tcaattcgca ttttgggatc atggacatgt gtggttttcc
      481 caagacggtg tattattatt accagtcctg gtggaccgat aaagatgttt tgcatatcgc
      541 gcccagctgg aatcggagcg gcaaggaaga ggggaagccg gtggccgtat gggtcaacag
      601 caatgcggag agtgtggagc tgatgctcaa cgggaagagt ttgggaaaga aggagatgcc
      661 gcggaatggg cacctggagt ggatggtgcc gtataccccg ggcaggctcg aggcggtggg
      721 atataagaag gggaggaaga tcacgacggt ggtggagacg acgggtgagc cgtaccggat
      781 cgtggtgacg ccgtcgaagg cggctctggc ggcggatggc cgggatgcga tggtctacaa
      841 ctgcagtgtt gtcgacaagc agggccggga ggttccggat gcgcagaacc tgttgcattt
      901 ttcggtcagc ggggatgcga aggtcatcgg cgtcggcaat ggggacccga gcagtcatga
      961 gccggatcaa tatcccgaag ggggctggca acggcatttg ttcaatggcc gctgccaggt
     1021 gatcgtgctg tcggcggcgg gggagcgagg gccgggtgcg gcaggggcgc ctgttcagtc
     1081 tgcggccggg ggtgtgcgcg gagcggccgg cgggaacggt gtattcttcg tgacgggtga
     1141 cgggctgctg ccttcgcaga cgccggtgcc attggggcat tgagggctgg gtataaataa
     1201 cctttatttt tggttctatg gcgatgtcct ccgaaataaa gaagcagatc gcagtggcgc
     1261 tgtattcgtc tcttgccgtc agtgtgttga cgacgacgcc ggtatttatg atgacgcgga
     1321 tatcctttag tctttatccc tggacgctgt tgtttgcgac gatgatgacg gtgatgatgt
     1381 gggggctcaa tatcctgttg ttctattttt cgggtggcag acgggtccgg cggtatatct
     1441 ggagctatgt ggtgtgtctg acctttgccc tggggatgtt tcacgggctg ctgaatatgc
     1501 cgatggacac ggggattggc tcagggggca ggggttttta ttttcatatc atcctcttct
     1561 tctcggtgga tacggtgttg ctgatcgtgc aggaccttgt ggttgtgcgg gaacggaagg
     1621 ccgtggtgga gctggagaat gcgcagttgc ggatgcggaa tatggaggcc gcgaatttgc
     1681 tgcttcagca gcaggtgcag ccgcatttcc tgttcaattc gctgagtacg ctgaagtcgc
     1741 tgatcaagta ttcgcctgcg gatgcggagg agtatgtggt gaagctgtcg ggttttttgc
     1801 ggagctcgat ggcgtcgcat gcatcgaagc tggtgaccgt ggagcaggag ctcgggttgt
     1861 gtaccgatta cctggagatg cagctgatcc ggtttggaga ggcgttgcgg tattcggtgg
     1921 aggtcgggga ggaggagcgg aggggttttt taccggtgtt cgcgctgcag ctgctgatcg
     1981 agaatgcgat caagcataat gtgctgacga aggagcggcc gttggctatc cgggtggagg
     2041 gcatggggaa gatggtgcgg gtggtgaata acctgcagct tcgggagggt gtggaggccg
     2101 gggggacggg attgacgaat ctgcgggagc ggtaccgggt gttgggcggc ggggaggtgg
     2161 ttattgcaac gagtgaaacc gaattttcag tcagtataag cctattgtct catgacgatc
     2221 gtgatcattg aagatgagcc gttgacggcg agggatctgg ctgcctgtgt gcgggtcgcg
     2281 gagccaactg tggagattgt ggccagcctg gcctctgtcg gagaggcgaa ggactggttc
     2341 gaccggcatg cggcgcctga tcttatcttt agcgatattc agctggggga tggtcaggca
     2401 tttgtagtgt tcgagcggct gatgcaaatg gtgccggtga tcttttgtac ggcctatgat
     2461 gaatatgcgc tggaggcgtt ccgggcggcg gggatcgatt atatcctgaa gccgttcacg
     2521 gagaagagtg tgggggcggc gctggcgaag tacaaggcct tgcggggagc agatcagacg
     2581 gggatggagg tggcgggtgg gaagatggcg gagccggccg cggcgggtag gaagatggtg
     2641 ggaccggttg cggccggtgg aaagatggcg gggccggccg gggcgatact ggtcttttat
     2701 aaagagaaga tcctgccggt gaaggtaggg gatattgcct ttttctacct ggagaaggag
     2761 gtggtg
//
LOCUS       DATGFG010000239         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1418914, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000239 DATGFG010000000
VERSION     DATGFG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418914"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..918
                     /locus_tag="VKQ52_04470"
     CDS             325..918
                     /locus_tag="VKQ52_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLZ86463.1"
                     /translation="MDTVFHLLDSIDTLEPGLRDYLYTALRTTQHDQGEAIVRESSIA
                     RTLGFIEKGLIRGFRINYKGNQFTSWFMREGDIYASVRSFFSQRPATETVQCLEPTIL
                     HSISFQQLQYILQKWPSFHRHRAELFEKYYLQSDEREEMRQGDAYSRFCYLMEHYPDL
                     VSRVQDQYLASFLNLTPTYYSDVKRRYFEENPNGGRR"
     gene            1046..1372
                     /locus_tag="VKQ52_04475"
     CDS             1046..1372
                     /locus_tag="VKQ52_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86464.1"
                     /translation="MKRKRVQKKLGIPMTEEEHQAFMKGYKATVYRSISAYARKLLLG
                     KPVQLIYRDRSLDDFIETAVKLRKELKLLLAKDSFTPSEKERLQQDITSIEENLIKLV
                     ETCCQK"
     gene            1357..2460
                     /locus_tag="VKQ52_04480"
     CDS             1357..2460
                     /locus_tag="VKQ52_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HLZ86465.1"
                     /translation="MLPKIEPNRQIRVSLRYNEKKVKEGSAERIGAENFLKDYDRLTK
                     EDILTRFRQRSSFNERLHDYGVHFSLNFGKQERLDNTKLVDIAGRYMNAMGFEDQPYV
                     VYRHSDAGHTHLHIVATTVRADGSWIRLEPRHYRASYALSRELEQQFALEKNQRTAPA
                     EQFRFAVDHAQKVKYGEPGLQRAISDVLNTVVDQYRYTSLDEFNAILKQYNVAANAGQ
                     ENSRLHKVGGLLYHALDEEGKRIGAPIKASRFLIKPTLKHLEQNFSENKQLRESSRER
                     LHTAIEWALAGRAPNWPQFTASLEKDGIAVVLNKNDDGKERIFFVDHVEKCAFAGERL
                     GANHDLPALRNRCAVEEQVQQQLSPEQQQKLHV"
     gene            2457..>2762
                     /locus_tag="VKQ52_04485"
     CDS             2457..>2762
                     /locus_tag="VKQ52_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YWFCY domain-containing protein"
                     /protein_id="HLZ86466.1"
                     /translation="MKTMEPSQQTTTISSYCTTASAIELLVHYFVVCYERFRQPGPFG
                     KVMDTLVETFRKFPLTGDEWITKGFVLILLIFPILFFGRQTDRPVRYKWPLIGLSAGL
                     "
BASE COUNT          754 a          810 c          646 g          552 t
ORIGIN      
        1 ggtaaacaat caccgcactg ccggaaatgc gcacctgctg cccctggagg acaaacgcat
       61 cggcgctgat aatattcaaa aagagtgata tatatgttga aactgatgac caccgcgcag
      121 gattaacgga tggacgtgca tccatggggt ctatccctgg aagccagcta cgtggaattt
      181 cacatggaaa ataccgtgaa aaaacgggga cgatttgcgc aaatccgcaa gcctttccgc
      241 gcccgaaagc cctctgtaaa atttcccctt atgcgcttcg cccgatcttt tccttccgta
      301 tcttccggta taaaccgcat ccttatggac acagtctttc atctcctcga ctccatcgac
      361 accctcgagc ccggcctccg cgattatctc tataccgccc tccgcaccac gcaacatgac
      421 cagggggaag ccatcgtaag agaaagctcc atcgcccgaa ccctcggctt tatcgaaaaa
      481 ggtcttatcc ggggtttccg catcaactac aaaggcaacc agttcacctc ctggttcatg
      541 agggaggggg atatttatgc gtctgtacgc agctttttca gccagcgacc ggcgacggag
      601 accgtacaat gcctcgaacc gaccatcctt cacagcatct ccttccagca actgcaatat
      661 atcctccaaa aatggccgtc cttccaccgg caccgcgccg aacttttcga gaaatactat
      721 ctccaaagcg acgagcggga agaaatgcgg cagggcgatg cctacagtcg cttctgctat
      781 cttatggaac attatccgga cctggtatcc cgggtacagg atcaatacct cgccagtttc
      841 ctcaacctca cacccaccta ctatagcgat gtcaaacgcc ggtacttcga agagaatcct
      901 aacggcggcc gccggtaagc cgctttttac catccgtctg acaacactta cgaaatcgta
      961 tttttttcgg gtactgcccg ccgtattttc attccgttga gcggccgaag gcctctcgcc
     1021 acagtcaccg gcaaacagga ccgttatgaa aagaaagcga gtgcaaaaga agctgggtat
     1081 ccctatgacg gaagaggaac accaggcatt catgaaggga tataaagcaa cggtttaccg
     1141 aagtatcagc gcatacgccc gaaagctgct cctcggtaaa cccgtccagc tcatctatcg
     1201 tgaccgttca ctcgatgatt tcatcgagac tgcggtaaaa ctgcgcaaag aacttaagct
     1261 cctgctcgca aaggacagct tcaccccatc agaaaaggaa aggctgcagc aggacataac
     1321 ctccatcgaa gaaaacctta tcaaacttgt cgaaacatgt tgccaaaaat agaaccaaac
     1381 cggcagatcc gggtttccct tcgctataat gaaaagaaag taaaggaagg cagcgcggaa
     1441 aggatcggcg cagaaaattt cttaaaggac tacgaccgac tcacaaaaga ggacatcctc
     1501 acccggttcc gccagcgatc ctcgtttaat gaacggcttc acgactatgg agtccatttt
     1561 agcctgaact ttggcaaaca ggaacggctt gacaatacga aattggtaga catcgccggt
     1621 cgctacatga acgcaatggg cttcgaagat cagccctatg tcgtctatcg ccactcggac
     1681 gccggccata cgcacctgca tattgtcgct accaccgtcc gggccgatgg aagctggatc
     1741 cggctggaac ccaggcacta ccgggcctcc tatgccctca gcagggaact cgaacaacag
     1801 tttgccctcg agaaaaacca acggacggcg ccggcagagc aattccggtt cgccgttgat
     1861 cacgcccaaa aagtaaagta tggcgaaccg gggttgcagc gtgccatcag cgacgtgctg
     1921 aacaccgtcg tcgaccagta tagatacacc tcccttgacg aattcaatgc catcctgaaa
     1981 caatataatg tggcggcaaa tgccggccag gaaaactcgc gcctgcacaa agtgggtggc
     2041 ctcctttacc atgccctcga cgaagagggt aaacgtatcg gcgcccccat caaggccagc
     2101 cgcttcctga tcaaaccgac actcaaacac ctcgaacaaa actttagcga gaacaagcaa
     2161 ctccgggaat cgtcccggga acgtctccat accgccatcg aatgggccct ggcaggccgg
     2221 gccccgaatt ggccgcaatt caccgcaagc ctggaaaaag acggcatcgc ggtggtcctg
     2281 aacaagaacg acgacggaaa ggaacgcatc ttcttcgtcg atcatgtcga aaaatgcgca
     2341 ttcgccggcg aaaggctggg cgccaatcac gatctccctg ccctgcgcaa caggtgcgca
     2401 gtggaagaac aggttcaaca acaactatca cccgaacaac agcaaaaatt acacgtatga
     2461 aaacgatgga accctctcaa cagacaacta caataagcag ctattgtacg acggcaagcg
     2521 ccatcgaatt gctcgtccat tattttgtcg tttgctacga aagatttcgc cagccagggc
     2581 cgttcggcaa ggtcatggat accctggtcg aaacattccg aaaatttccc ttaaccggcg
     2641 acgaatggat cacgaagggc tttgtactta tactactgat attcccgatc ttgttcttcg
     2701 gccggcagac agaccggccc gtaaggtaca aatggccgct gatcggtctc agcgccggtc
     2761 tc
//
LOCUS       DATGFG010000240         3692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_499507, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000240 DATGFG010000000
VERSION     DATGFG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499507"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VKQ52_04490"
     CDS             complement(<1..630)
                     /locus_tag="VKQ52_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86467.1"
                     /translation="MRFPITGCLLFVFAFFVACHSTKRGAADSWAVYGGSQANTHYTS
                     LTVLDTGNVSGLRQVWEYHTRDGDSLSQIQVNPLVIDGVLYGVSPKLKLFALNAATGE
                     MRWVFDPAAPVNGDTPKIAINACRGVAYYSGRPADGAGGSGADSATDQRLFYGAGSYL
                     YCIDAATGKPAAGFGDSGRIDLHHDLGRDVRDLYVAATTPGMVYKDMIII"
     gene            876..3248
                     /locus_tag="VKQ52_04495"
     CDS             876..3248
                     /locus_tag="VKQ52_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86468.1"
                     /translation="MFKNYLHIALRQLRKNRVYSFINIAGLATGMAIALIIGIWITGE
                     LSRDHDYPDGDRIAEIMQDQHGKAEGKAYSYTGKTLSSALKPFLQKGYNDVIAQSAIT
                     LWPGDALLVTGDKSISRKSFWVEYTFPLIFGYHFLSGTAESMRDPNTAVISRSTAIAL
                     YGTENAVGKTFKLSNQTPLTVGGVFANPPANSTFHDVEVLASIANDNCKWLMNNTDFE
                     NHSCNIYVRLAGRTTAEQATARIKNICTPFVKYATETYRVMPFEQLYLHYDDTNASTE
                     TGAIGFVRLIGIIGVFVLLLACINFMNLSTARSEQRAKEVGIRKTIGSLRSQLIAQFL
                     GESILLAAISFVLAILLAAFSLPWFSVLAGKTMLFPWASPVFWGLSGAFTILTGLIAG
                     SYPAFYLSGFRPVKVLKGAFKAGRGASTPRKVLVVAQFSISLALIIGTIVVFRQIQLA
                     KDRPLGYDRAGLITVPINTDSLDRHYESLRTALLNSGVIANMASSSQTLNSFPQNNFM
                     EWEGMTLEQKHTSFRDIFVSADFGPTIGWTIVKGRDFSRAFRTDSTAAIVNEEGARIL
                     GFKDPIGKTVKHFGKNVTIIGVSKDMVNNNVYGKVQPAIFLGEGGHNVFTIRIKPGIP
                     VHAALARMESVFKQYNPASPFIYQFNDADFEQKYSTETRIGNLATVFSGLAILISCLG
                     LFGLASFVAEQRTKEIGVRKVLGAGVVDLWALLSSEFLKLIVLSMLIAMPLAYYCMSQ
                     WLQNYPIHADLSAWIFLASGAGILSITLATVSYQALKAALMNPVKSLRTE"
     gene            complement(3264..>3692)
                     /locus_tag="VKQ52_04500"
     CDS             complement(3264..>3692)
                     /locus_tag="VKQ52_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86469.1"
                     /translation="QLLDRIKMVFPAIPDGWDWDVAMPPFATNYTVLYEREARGLPGR
                     YNFYASLERADPAAVAAAFENQGWAMRKSGGEEMEANLSWGEISIVKDKQEVLLNGRV
                     DFRPDHVEDLHHLLDGIAGVYQYEFYDEEGRVLLERRRPG"
BASE COUNT          874 a         1217 c          834 g          767 t
ORIGIN      
        1 gataatgatc atatccttgt agaccatccc cggcgtggtc gccgccacat acaggtcccg
       61 cacatccctc cccaggtcat ggtgcagatc gatccgtccg ctgtcgccaa aacccgccgc
      121 cggcttcccc gtcgccgcat cgatgcagta caggtaagac cccgcgccat aaaaaagccg
      181 ctgatccgtg gcgctgtcgg ctccgctgcc gcccgctccg tccgccggcc gcccggaata
      241 gtacgcaacc ccgcgacagg cattgatcgc gatctttggc gtatcaccat tcaccggcgc
      301 ggcaggatcg aacacccacc gcatttcacc cgtggccgca ttcaacgcaa aaagcttcag
      361 cttcggcgac acgccgtata aaacgccatc gatcaccagc gggttcacct gtatctgcga
      421 aagactgtcc ccatcccggg tatggtactc ccacacctgc cgcaacccgg agacatttcc
      481 cgtatccaat actgttaagg aggtgtaatg ggtattcgcc tggctgccac cataaacggc
      541 ccaggaatcg gcagcaccac gcttcgtgga atgacaggca acgaaaaaag caaacacaaa
      601 aagcaggcaa ccggtaatgg gaaaacgcat gcgttctttt gagaatgaat atacataaat
      661 ttgtcccatg acgccggcaa tcaccgggtc gcctgccccc cttcgtggac aaccacaaca
      721 gctacgcctt cgacaaaaag ttccgcaact gaacatagag tgtgcgaaac cggacaatta
      781 ataaaagcaa agaaatcgca accaattgat tattaagaag taaaaaaaca ggtacttttc
      841 tggctacagt tctgctgtat tctgaaacta ataccatgtt caaaaactat ctccacatcg
      901 ccctccggca actccgcaaa aaccgcgtct actcctttat caacattgcc ggcctcgcca
      961 ccggcatggc catcgctctc atcatcggca tctggatcac gggcgaactc tcccgggacc
     1021 acgattatcc cgacggcgac cgcatcgcgg agatcatgca ggaccaacac ggcaaggccg
     1081 agggtaaagc gtactcctac acgggcaaga cactctcttc ggccctcaaa ccgttcctgc
     1141 aaaaaggcta caatgacgtc atcgcccaat cggccatcac gctctggccc ggcgacgcac
     1201 tgctggtcac cggggataaa agcatcagcc ggaaaagctt ctgggtggaa tataccttcc
     1261 ccctcatctt tggctatcat ttcctcagcg ggaccgcgga atccatgcgc gaccccaata
     1321 cggccgttat ctcccgctcc accgccatcg cactctatgg caccgaaaat gccgttggca
     1381 agacgttcaa actgagtaac caaaccccgc ttaccgtcgg cggcgtcttt gccaacccgc
     1441 ccgccaacag caccttccat gatgtcgagg tcctcgcctc cattgccaat gacaattgca
     1501 aatggctgat gaacaacacg gattttgaga accacagctg caatatctat gtccgcctgg
     1561 ccggcaggac aaccgcggaa caggctacag cccggataaa gaatatctgc acacccttcg
     1621 tcaaatatgc caccgaaacc taccgggtca tgccgttcga acaattgtat ttgcattatg
     1681 acgacaccaa cgccagtacg gaaacgggtg ccatcggctt cgtccggctc atcggcatca
     1741 tcggggtctt cgtcctgttg ctggcctgca tcaacttcat gaacctttca acagcccgca
     1801 gtgaacaacg cgcaaaggaa gtgggcatcc gaaagaccat cggctcgctg cgcagccagc
     1861 tcatcgccca gttccttggt gaatccatcc tgcttgccgc catctctttt gtcctggcga
     1921 tcctcctggc cgctttttca cttccctggt ttagtgtgct cgccggcaag accatgctct
     1981 ttccctgggc cagcccggtc ttttggggac tctccggggc atttacgatc ctcaccggcc
     2041 ttatcgccgg cagctacccg gccttctacc tgtctggttt ccgtcccgtc aaagtcttaa
     2101 aaggcgcgtt taaagccggc cgcggcgcca gcaccccacg aaaagtcctc gtcgtggcgc
     2161 agttctccat ttcactggcg ctgatcatcg gcacgatagt cgtctttcgg cagatccagc
     2221 tcgcgaagga tcgccccctt ggctatgacc gcgccgggct cattacggtg cccatcaaca
     2281 ccgactccct cgatcgccac tacgagtccc tgcgcacagc cctcctcaac agcggcgtca
     2341 tcgccaatat ggccagctct tcccaaacac tgaatagttt cccccagaat aattttatgg
     2401 aatgggaggg aatgaccctc gaacaaaaac atacctcctt ccgcgatata tttgttagcg
     2461 ccgatttcgg accaacgatt ggctggacca tcgtgaaggg ccgcgatttc tcccgtgcct
     2521 tccgcacaga cagcacagcg gcgatcgtca acgaagaggg ggcccgcatc ctcggattca
     2581 aggaccccat cggcaagacg gtaaaacact ttggcaaaaa tgttaccatc atcggagtat
     2641 cgaaggatat ggtgaacaac aacgtatacg gtaaggtcca accggccatc ttcctcggtg
     2701 aaggggggca caatgtgttc accatccgta taaaacccgg aatacccgtt cacgccgctt
     2761 tagccaggat ggaatccgtg ttcaagcaat ataacccggc cagtccgttc atctatcaat
     2821 tcaatgacgc ggatttcgaa cagaaatata gcacggaaac ccgcatcgga aatctcgcca
     2881 cggtgttctc gggcctcgcc atccttattt cctgcctcgg actcttcggc ctggcctcct
     2941 tcgtcgccga acaacgcaca aaagagattg gcgtccgcaa agtcctgggc gccggcgtgg
     3001 tcgatctctg ggcattgctt tccagcgaat tcctgaagct catcgtcctg tccatgctca
     3061 tcgcgatgcc ccttgcctac tactgcatga gccaatggct ccagaactac ccgatccatg
     3121 ccgatctctc agcctggatc ttcctggctt ccggcgccgg tatcctctcg atcaccctcg
     3181 ccaccgtcag ctatcaagcc ctcaaagcag ccctgatgaa tcccgtcaaa agtctccgca
     3241 ccgagtgatt ttgactagta caattaaccc ggccgccgcc gctccagcag cacccggccc
     3301 tcctcatcat aaaactcata ttgataaacc cccgctatcc catccagcaa atgatgtagg
     3361 tcttccacat gatcagggcg aaaatccacc ctgccattca gcagcacctc ctgcttgtcc
     3421 ttaacaatgg atatctctcc ccaggagaga tttgcctcca tctcctcccc gccggatttc
     3481 cgcatcgccc atccctgatt ttcaaaagcc gcggccactg cagccggatc cgcccgttca
     3541 agactggcgt aaaaattata ccggcccggc agaccccggg cttcccgctc atacaacacc
     3601 gtatagttag tagcaaaagg aggcatggcg acatcccaat cccaaccatc cgggattgcc
     3661 ggaaacacca tttttattct gtcaagaagc tg
//
LOCUS       DATGFG010000241         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1587016, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000241 DATGFG010000000
VERSION     DATGFG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587016"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2308)
                     /locus_tag="VKQ52_04505"
     CDS             complement(<1..2308)
                     /locus_tag="VKQ52_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86470.1"
                     /translation="MNTILKPALRQLRKYPIFSLINLGGLAIGIAASFILLVYTQREL
                     STDHHWRDADRIARIGTDFFQLGPFAFSQPMLRDLAIASCKDVEDATGFDMNPYPVRT
                     TRDDRAFTGIQSYLIDSSFFHVFSFETAAGTILRKGLAPNQAILSSTQAKKFFGDNQA
                     IGQTIFVGKELTPYTIIAVLKESFAKSTIDPQILLPIPKRTAAEPAGWMSSQVYNFVK
                     LKPQGTLAGLDAWLERLREKVVYPASGSTDSYKVWKTKGTSVFFYTKHLTDIYFHSEC
                     KFDDFEGGNLTQVQLLSAISILLILLAIINYVNLVTARSSIRSKEIGLKKTFGAPRRE
                     LIGQLLKESLLFSGLAMLLSCGLIQVILFLYQYSTGATLTGPIPFLSANYLWLIAFSI
                     GVGLIAGIYPAIYLTGIRPGLAVRSTSGSGKNLPLLRNSLVALQFIIAIGLLFVSLVV
                     YSQLQYMKNKDKGLRTDGVVLVEDPGDINNPEVARKFQTLKTLVDQQAQVTSSSLCHR
                     APAGHTMTMGSYRSPATKKEVLLQTFQADEKYMSTLGLHLTDGRNFTKGLLSDTNSLI
                     LNESAVAAMGLFNPVGTLLNGSERVIGVVKDFNYASMQEKIAPAVIRFSPAGNTTLAI
                     HVSSAHPGAFLDWLKEKSKTIIPDENVNINFLDENFGRLAAKEKLLGQAITFFTALAI
                     LLATLGLIGLTLFTIERRTKEIGIRQVLGAGNGNILRLISRDFIRLALIASVIALPLS
                     WWLIHRWLENFAYRVTVSAGIFLLTETLI"
BASE COUNT          434 a          467 c          958 g          547 t
ORIGIN      
        1 ggatcagggt ttcggtcagc aggaagatgc cggcgctgac ggttacgcgg taggcgaagt
       61 tttccagcca gcggtggatg agccaccagc tcagcgggag ggcgatgacc gaagcgatga
      121 gtgcgagccg gatgaaatcc ctggagatga ggcggaggat gttgccgttg ccggcgccga
      181 ggacctggcg gatgccgatc tcctttgtcc ggcgttcgat ggtgaataag gtgaggccga
      241 tgaggcccag tgtggcgagg aggatggcca gggccgtgaa gaaggtgatg gcctggccca
      301 ggagtttttc ttttgcggcg aggcggccga agttctcgtc gaggaagttg atgtttacgt
      361 tctcgtcggg gatgatggtc ttgctttttt cttttagcca gtcgaggaag gcgccggggt
      421 gggcggagga gacgtggatg gcgagggtgg tgttgccggc gggggagaag cggatgacgg
      481 cgggggcgat cttttcctgc atggaggcgt agttgaagtc tttgacgacg ccgatgacgc
      541 gttcgctgcc gttgagcagt gtcccgacgg ggttgaagag ccccatggcg gcgacggccg
      601 attcgttgag gataagggag ttggtatcac tgagcagtcc ttttgtgaag ttgcggccgt
      661 cggtgagatg gaggccgagg gtcgacatgt atttttcgtc ggcctggaat gtttgcaaca
      721 ggacttcttt ttttgtcgcg gggctgcggt agctgcccat tgtcatggtg tgaccggcgg
      781 gtgcgcggtg gcagaggctg gagctggtga cctgggcttg ctggtcgacg agggttttga
      841 gggtttggaa tttccgggcg acttcggggt tgttgatgtc cccgggatct tctacgagga
      901 cgacgccgtc tgtgcggagg cctttgtcct tgtttttcat gtattgcaac tggctgtaga
      961 cgacgagcga gacgaagagc aggccgatgg cgatgatgaa ttggagggcg acgaggctat
     1021 tgcgaagaag ggggaggttc ttacccgacc cggacgtgga gcgaacggcg aggcccggcc
     1081 ggattcccgt gaggtaaatg gcggggtaga tgccggcgat gaggccgacc ccgatagaga
     1141 aggcgatgag ccagagataa tttgcggaca ggaaggggat gggccctgtg agggtggcgc
     1201 ccgtggaata ttgatagagg aagaggatga cctggatgag gccgcaggac aggagcatgg
     1261 cgaggccgct gaagagcagg gattccttca gcagttggcc gatgagttcg cggcgggggg
     1321 cgccgaaggt ctttttgagg ccgatctctt ttgagcggat ggaggagcgg gcggtgacga
     1381 ggttgacata attgatgatc gccagcagga tgaggaggat gctgatggcg ctgaggagtt
     1441 gaacctgggt gaggttgccg ccttcgaaat cgtcaaattt gcattccgag tggaagtaaa
     1501 tgtctgtgag gtgtttggta tagaagaaga cggaggtgcc ttttgttttc cagactttgt
     1561 aggagtcggt cgagccgctg gcggggtaga cgaccttttc gcggaggcgt tcgagccagg
     1621 cgtcgaggcc ggcgagggtt ccctggggtt ttagcttgac gaagttatag acctgggagg
     1681 acatccagcc ggctggttcg gcggctgtgc gtttaggtat gggcaggagg atctgggggt
     1741 cgatggtgga tttggcgaag gattctttga ggacggcgat gatcgtgtag ggggtgagct
     1801 ctttgccgac aaagatggtt tgaccaatgg cttggttatc gccgaagaat ttttttgcct
     1861 gggtggacga gaggatggcc tggtttgggg cgaggccttt tcggaggatg gtgcctgcgg
     1921 ccgtttcgaa ggagaagacg tggaagaagg aggagtcgat gaggtagctt tgaatgccgg
     1981 tgaaggcgcg gtcgtcgcgg gtggtgcgga caggataggg gttcatgtcg aagccggtgg
     2041 cgtcttcgac gtctttgcag gaggcgatgg ccaggtcgcg gagcatgggt tggctgaagg
     2101 cgaaggggcc gagctggaag aagtcggtgc cgatgcgggc gatgcggtcg gcgtcgcgcc
     2161 agtggtggtc ggtgcttagt tcccgctggg tatagacgag gaggataaag gaggcggcga
     2221 tgccgatggc gaggccaccc aggttgatca gggagaatat ggggtatttg cggagttggc
     2281 ggagggctgg ttttaggatg gtgttcatag ttaaaactaa ggattttttg gtttcgtcgg
     2341 gttgttgccc ggctgttcgc tgttcggcga gggttggggg gatggtttct ggtggttagt
     2401 cggttc
//
LOCUS       DATGFG010000242         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_380801, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000242 DATGFG010000000
VERSION     DATGFG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380801"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /gene="pbpC"
                     /locus_tag="VKQ52_04510"
     CDS             complement(<1..1860)
                     /gene="pbpC"
                     /locus_tag="VKQ52_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HLZ86471.1"
                     /translation="MRRQSIIRRLKVAGIVVAGLFLLFLLLNWVFPLPDRIEYSTIIT
                     DDKGEVIHAFLTHDQQWRMKTELSDISPLLRKTIVEKEDKYFYYHLGINPAAMCRAFG
                     RNLLLMRRTSGASTITMQVARALEPKKRTYWNKVREMFRALQLEWKYSKDDILQIYLN
                     KVPYGGNIQGVKSASILYFRKNPDHLSLAEITALSIIPNRPSSLVMGRNNDRIVEERN
                     KWLRRWAREKVFTEKEIADALAEPLTATRGTVPTLIPQLAQKLKKHGGPIVHSTIQLN
                     TQLKLEKLVKDYSRTLALKNIRNAAVIVLDNQTHRVVSYVGSADFHDSADAGQVNGAA
                     AIRQPGSTLKPLLYGLCIDEGLLTPRRMITDVPVNYDGYAPENYDRRFNGYVTMEYAL
                     EHSLNIPAVKSLKALGKEKLIRKLTDCDFRQVDHDRKKLGLSMILGGCGATLEELTGL
                     YSIFANEGRYVRPQYVWGDTVVKGKTVDGGAAAGQKILSPAATFMINETLSKVNRPDF
                     PLNWESTVHLPKIAWKTGTSYGRRDAWSIGYDKRYTVGIWVGNFSGLGIPELSGANVA
                     TPLLFTVFNTIDYDDDASWFSKPEDCDVRMVCSETGLPPADFCTHLVTDYFIPL"
     gene            2105..>3212
                     /locus_tag="VKQ52_04515"
     CDS             2105..>3212
                     /locus_tag="VKQ52_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86472.1"
                     /translation="MRNRSGLTALLMTALPVLLIFSSCNRSTVSLEYTNAKDEVPTLG
                     NLIFRFDQPLVKDSLLDQWDSTQYISFEPKIPGRFRWEHPDELVFSPSQPLAPATTFK
                     AILKSDILQYSKFGRIKKGEDILFHTPDLKLDNTSATWVLPDETSTLAVPKMELQFNY
                     PVNPNSLKDKLTILLSGQPVNYTLQTLSATERIALRLTNLKMEDKNLDIKLTVDKGLL
                     PEGGTNGLKDKIEASTIIPSPYVLTVGEVTTDHDGSTGSIHIQTSQQVLEGGLASAIS
                     FSPAIKFTAFPTDNGFTIRSDNFDQEKSYTLTLAKGLRGRIGGTLHEEYSTNITFGEL
                     APSISFANNKGVYLSGKGAQNIEVKIINVPRVKIV"
BASE COUNT          726 a         1060 c          720 g          706 t
ORIGIN      
        1 cagcgggatg aaataatcgg tcacgagatg cgtacagaaa tccgccggcg gcagccccgt
       61 ctcggagcat accatccgca catcgcaatc ctccggcttc gagaaccagg aggcatcgtc
      121 atcatagtcg atggtattga aaacggtgaa caacaacggt gtcgccacat tggccccact
      181 cagctcggga atgcccagcc cggaaaaatt cccgacccag atacccaccg tatagcgttt
      241 gtcatacccg atgctccatg catcccgccg tccataagac gttccggtct tccacgcgat
      301 cttcggcaga tgcaccgtgc tctcccagtt gagcgggaaa tccggccggt tcaccttcga
      361 aagcgtctcg ttgatcataa aggtcgccgc ggggctcagg atcttctgcc cggcagccgc
      421 tccaccgtcc acggtcttcc ccttcaccac agtgtccccc caaacatact gtggccgaac
      481 atatctcccc tcgttggcga atatcgaata caaccccgtc agctcctcca gggtcgcccc
      541 gcaaccaccc aggatcatcg ataacccaag ctttttccgg tcgtgatcca cctgccggaa
      601 atcacagtcc gtcagtttcc ggatcagctt ttccttccca agggccttca ggctcttcac
      661 cgccgggata ttcagggaat gctccagcgc atactccatc gtgacatacc cgttgaaacg
      721 ccggtcgtag ttctccggcg catacccgtc ataattcacc ggcacatcgg tgatcatccg
      781 cctgggcgtt agcaacccct catcgataca aagcccgtac aataacggct ttaatgtact
      841 tcccggctga cggatcgccg ccgccccatt cacctgcccc gcatcggccg aatcatggaa
      901 atccgcgctc cccacataac tcaccacccg gtgcgtctgg ttatccagca cgatcaccgc
      961 cgcattacgg atattcttca acgcaagtgt ccgcgaatag tcctttacca gcttctccag
     1021 cttgagctgg gtgttgagtt ggatcgtact gtggacgatc ggccccccgt gcttctttaa
     1081 tttctgcgcc agttgcggga tgagggtggg gaccgtgccc ctggtcgccg tcagcggctc
     1141 ggccagcgca tcggcgatct ccttttccgt aaacaccttc tccctcgccc accgccgcag
     1201 ccacttgttc cgctcttcga cgatccggtc gttgttccgc cccatcacca gcgaagaagg
     1261 ccggttgggg atgatggaca atgccgtgat ctccgccagc gacagatggt cggggttctt
     1321 cctgaaatac aggatcgacg ccgactttac cccctggata ttgccgccat agggaacctt
     1381 gttcaggtag atctgcagga tatcatcctt ggaatacttc cactccagct gcagggcccg
     1441 gaacatctcc cgcaccttat tccagtaggt gcgcttcttc ggctccagcg cccgtgctac
     1501 ctgcatcgtg atcgtggatg ccccggaggt gcgccgcatc agcagcaggt tcctgccaaa
     1561 ggccctgcac atcgccgccg ggtttatgcc caggtgataa tagaagtatt tgtcctcttt
     1621 ctcgacgatg gtcttgcgca acaacggcga gatatccgac aactccgtct tcatccgcca
     1681 ttgctggtca tgggtgagaa aggcatggat cacctctccc ttatcatccg tgataatggt
     1741 ggagtactcg atcctgtccg gtaagggaaa cacccaattc aacagcagga aaagtaagaa
     1801 tagtcccgca acaacgatcc cggcgacctt cagccgccgg ataatgctct gccgcctcat
     1861 aaaaacgcaa ttaacaattt cttatcatat gaaaacttgc aaaagggcct gttgtgattg
     1921 taatttcata ctggcattaa tgccgcttta tgtacgcccc caccatccct ccttagcttc
     1981 tcacataaaa ttctgtcttt cctcattagc ctgcaagcaa acagacagct tttttttatt
     2041 gccttttcgt atattaacat tgcctttaaa agcgttttca ctcaaacact tatcagactc
     2101 ctatatgcgt aaccgttcgg gcctcaccgc cctcctcatg acagctctcc ctgtcctgct
     2161 gatcttttct tcctgtaacc gtagcaccgt cagcctggaa tacaccaacg ccaaagacga
     2221 ggttccgaca ctgggcaacc ttatcttccg cttcgaccaa cccctggtca aagactccct
     2281 gctcgatcaa tgggactcta cccagtacat ctctttcgaa ccaaagatcc ccggccgctt
     2341 ccgctgggaa catcccgatg aactcgtttt ctccccttcc cagccgctcg cgccggccac
     2401 caccttcaag gctatactaa aaagcgatat cctgcagtac agcaagttcg gccggataaa
     2461 aaaaggggaa gacatcctct tccacacgcc cgatctcaaa ctggataaca cgagcgccac
     2521 ctgggtattg cccgacgaga ccagcaccct tgccgtaccc aagatggaac tccagttcaa
     2581 ctaccctgtc aaccccaatt ccctgaagga taagctgacg atcctacttt ccggccagcc
     2641 cgtcaactat accctccaga ccctttccgc caccgaacgc atcgccctgc gcctcaccaa
     2701 tctgaagatg gaggataaga acctcgatat aaaactgacc gttgataaag gcctccttcc
     2761 cgaaggcggc accaacgggc tgaaggacaa gatcgaggcc tcgacgatca tcccctcgcc
     2821 ctatgtcctg actgtcggcg aggtaaccac ggaccatgat ggttccacgg gctccatcca
     2881 tatccagaca agccagcagg tgctcgaagg cggtcttgct tccgccatca gcttcagccc
     2941 ggccatcaaa ttcaccgcct ttcccacgga taacgggttc accatccgca gtgacaactt
     3001 cgaccaggaa aagtcgtata ccctcacgct cgccaagggc ctgcgcggcc gcatcggcgg
     3061 aaccctgcac gaagaataca gcaccaatat cacttttggc gaactggcgc cttccatcag
     3121 cttcgccaac aacaaaggtg tttacctctc cggcaagggg gcgcagaaca tcgaggtaaa
     3181 gatcatcaat gtgccccgcg tcaagatcgt cg
//
LOCUS       DATGFG010000243         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_259235, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000243 DATGFG010000000
VERSION     DATGFG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259235"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..1954
                     /gene="istA"
                     /locus_tag="VKQ52_04520"
     CDS             428..1954
                     /gene="istA"
                     /locus_tag="VKQ52_04520"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HLZ86473.1"
                     /translation="MSKLKQILLQRSNGISLQTISKTLELSRNTVKKYLRLIDVRGYD
                     PAELLAMSEEALEALLEEPDEPSAERQRSLEELFPYFEQELQRTGVNRGVLWSEYRQQ
                     YPDGYGYSQFCERFKQWAVTQAATLHLEHHPGDKLFLDFTGKKMSIVDRETGEQIPVE
                     IFIAILGYSQLTYAQAVFTQQLLDCLRAVENAFLYFGGLTRVIVPDNMKTAVHKADKF
                     EAQLNKLFLELATHYQTSVLPARSYKPRDKSLVEGAVNIAYTRIFAPLRNQVFYSLKD
                     LNEAIAGLLEAHNNQPFQRKPQSRRQIFETEERSTLLPLAAERFEIKQYREVTVMKNC
                     HVHLHDDHHYYSVPYRYIGKKVKLVYSASQVAVFYNRERIACHSRERKAYGYTTHKQH
                     LPSTHQFVSDWNPDKFIGWAASIDPVVKDYITRILDSKAYPEQTYRSCVGILSQERKV
                     GRDRLIKAITRATEFGAYNYTTVHKILTLGLDRLDEPAEPAQAKLPFHENIRGAGHYQ
                     "
     gene            1985..2710
                     /gene="istB"
                     /locus_tag="VKQ52_04525"
     CDS             1985..2710
                     /gene="istB"
                     /locus_tag="VKQ52_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HLZ86474.1"
                     /translation="MSTTILNKLSQMKLHGMWHAFKTLQESHHSQELTHDELVDLLVQ
                     AEWEQRENKKFNRYLRQARFRYMASVEEVDFTALRGLDKSQFLRLAEGGFVHKRENLL
                     ITGATGVGKSYLASALGHQACLLGYRTLYFNTQKLFSRLKMLKADGSYGREINKIERA
                     DLVILDDFGMQPLDNGSRMIFLEIIEDRHGRRSTLIGSQLPVSKWYEIIGDSTIADAI
                     LDRMVHTAHRIELKGESMRKTKK"
BASE COUNT          731 a          789 c          715 g          603 t
ORIGIN      
        1 acaaagctac gcaaggttta agttagacag aaatgtttgt ggtgagatcc gtttggatta
       61 ataggatgga gatttacaag cgaccggtcc taataaataa gtgtccggac ggggtacaag
      121 atagacaaat cccaataaca acgaaaaaac cctggcgcct tttttttcgt atgtaaaagg
      181 ggtacatgga gattccgacc catgttgacc acccgttccg accatcctga ccacccattc
      241 cgatgatgtt gaccatccct gccggttcgg cagcggtgtc attccggcaa tgttgaccac
      301 ccccgttcgg gcggtttttc tgacattccg acgatattga ccacccctcc gggtgcctac
      361 tctgtaatcg ttttcttgct ttgcagggaa ttaaaccctg catgacatgg ccggcaaaac
      421 gattatgatg agtaaactca aacagatcct gctgcaacgc agcaatggaa ttagcctaca
      481 gaccatcagt aagaccctgg aactctcccg caataccgtg aagaagtatt tgcggctgat
      541 cgatgtgcga ggttacgacc cggcggaact gctggcgatg agcgaagaag cgctggaagc
      601 attgctggaa gaaccagatg aaccttcggc ggagcgtcag cgttccctgg aagagttgtt
      661 cccctacttc gaacaggagt tgcaacgcac cggcgtgaac cgcggggtgc tttggagcga
      721 ataccggcag caatatcccg atggttatgg ttactcacag ttctgcgaac gatttaagca
      781 atgggcagtc actcaggctg ccacgctcca cctggaacac catccgggcg acaagctgtt
      841 cctggacttt accggcaaaa agatgtcgat cgttgaccgg gagaccggcg agcagatccc
      901 cgttgaaatc ttcattgcta tcctggggta cagccagttg acgtatgcac aggctgtatt
      961 tactcagcaa ctgctggatt gtctccgtgc cgtggagaat gccttccttt acttcggtgg
     1021 cttgacgcgg gtaatcgttc cggataacat gaaaacggcc gttcacaagg cagataaatt
     1081 tgaagcacag cttaataagc tgttcctgga actggccacc cattatcaga ccagcgtact
     1141 gcctgcccgc agctacaagc ccagggacaa gtcgctggtg gaaggagccg tcaacattgc
     1201 atatacgcgg atttttgcgc ccttgcgcaa ccaggtcttc tacagcctga aggacctcaa
     1261 tgaggcgatt gccggtctgc tggaagccca taacaaccag cccttccagc gcaagcccca
     1321 gagccgccga cagatcttcg agaccgaaga acggtccacc ctgctgccgc tggcggctga
     1381 acgttttgag attaagcaat accgcgaggt gaccgtcatg aagaactgcc atgtgcatct
     1441 gcatgatgac catcactact atagtgttcc ctatcgctat atcggcaaaa aagtaaagct
     1501 ggtctactct gcttctcagg tggccgtttt ctacaatcgc gagcgaatcg cctgtcacag
     1561 ccgggaaaga aaggcctacg gctataccac ccataaacag caccttccgt cgacacacca
     1621 gttcgtcagc gactggaacc ccgacaagtt catcgggtgg gcagcatcca ttgatccggt
     1681 ggtcaaagat tatatcaccc gtatccttga tagcaaggcc taccctgaac agacctatcg
     1741 cagctgtgtg ggcatcctct ctcaggaaag aaaagtcgga cgggatcggc tcatcaaagc
     1801 cattacccgg gccaccgagt tcggagccta taactatact accgttcaca aaatactgac
     1861 cctcgggtta gaccggcttg atgagccggc ggagccggcc caggctaagc tgcccttcca
     1921 cgagaacatc cgcggcgccg gacactatca ataatcttca ttacttaata aatacacaac
     1981 gattatgagc accacaatcc tcaacaaact ctcccaaatg aaactccatg gcatgtggca
     2041 tgccttcaag acgctgcagg agagccatca cagtcaggaa ctgactcacg acgaactggt
     2101 ggacctgctg gtccaggccg aatgggaaca acgggaaaac aaaaagttca accgctatct
     2161 acggcaggcc cgcttccgat atatggccag cgtcgaagag gtggatttta cagccctgcg
     2221 gggcctggac aagagccagt tccttcggtt ggcagaaggc ggcttcgtac acaaacggga
     2281 gaacctgctg atcactgggg cgaccggcgt cggaaaaagc tacctggcct ccgcgctggg
     2341 gcaccaggcc tgcctgctag gctaccgcac cctttatttt aacacccaga agctcttctc
     2401 ccggctgaag atgctaaagg ccgatggctc ttacggccgg gagatcaaca aaattgaacg
     2461 tgctgatctg gtcatcctcg acgactttgg tatgcagccg ctggacaacg gcagccgcat
     2521 gatcttcctg gagatcatcg aggatcgcca tggccgccga tctaccctga tcggctcaca
     2581 gttacccgta tccaaatggt atgagattat cggcgacagc acgattgcag atgccatcct
     2641 cgatcgcatg gttcacaccg ctcaccgtat cgagttaaaa ggcgaatcca tgagaaagac
     2701 caaaaaataa ctaccttgta ccttagacct gcaagaatgt cattacagag caggggctgc
     2761 cctaacacct atggggtggt caacatcgcc ggaacgcatg gtcaacatgc ccggaatccc
     2821 caatatttgc tggaaagc
//
LOCUS       DATGFG010000244         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_146586, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000244 DATGFG010000000
VERSION     DATGFG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146586"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VKQ52_04530"
     CDS             <1..440
                     /locus_tag="VKQ52_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86475.1"
                     /translation="GVNGYGGNDFPVMRYADVILMLAEVSMYQGNAAQAIQYLNIVRT
                     RAKMPDYATSMLNPAYAAKFPTLKLAILHERRSELAFEHHRLFDLLRFFNVNDLVTYI
                     HSKNAADWGLANIANFGTKDEYYPIPYNEVILDPKRMYQNPGY"
     gene            652..2238
                     /locus_tag="VKQ52_04535"
     CDS             652..2238
                     /locus_tag="VKQ52_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HLZ86476.1"
                     /translation="MNSLTRREFLRRTGVLAGGAYPAMMAMGMVPAAPVRPFRLEGSG
                     GGKEVVILGAGLAGMAAAYELGKLGYHCTILEARERAGGRCWSIRKGSVNEETGGGSH
                     TAGFDEGQYFNAGPSRIPHHHGLTLHYCKELNVPVQVYNNVNENTYYYSEGKGPLSNK
                     KVRVREVHNDMRGWMAELLAKGMDGGKIDAGMSREDTLKVIEYLRAEGGLDIDKLYKA
                     SARRGYIESPGPGDREGKLGDPSTLLAMLQSGLLDPDFYNVAEYTYELQGTLFQAVGG
                     MDRIAAAFEQRVGNLIRYQAEVSKIHNLEQGVEVGYKDKTGEKTIKAEFCICTIPLPV
                     LSNIDHNFASDASRAIDSIVYMSTGKIGLQFKRRFWEEDEGIYGGITHTNNQLTQIFY
                     PSYDYLSGKGILIGYYNFNEKAVATGNLSYAQREQLALDKGSLIHPQYRQEFETSFSV
                     SWHKTKYNLGGWALYSAEERQTLYKALLQPDKQVYFAGEHMTYLNAWMAGALESARSV
                     VAALHSRVTGQHFSYPGFGS"
     gene            2290..3474
                     /locus_tag="VKQ52_04540"
     CDS             2290..3474
                     /locus_tag="VKQ52_04540"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HLZ86477.1"
                     /translation="MGNSISRRTLLKRGALLAGGLPFAASLLDKVQASPLIVKPGESA
                     VPYTEAWTEKQIALNAPAPLKARLFANENPFGPSDKAKKAIMEACPTSYQYPFRAMPD
                     LIKKIAAFEGVKEENILLASGSSPLLMASALYFSKPGGNIITGDPSYEDLPSKAVRMG
                     EKWVKVPLTADFKLDLVAMEKAIDENTGLVYVCNPNNPTATVLDTAQLKAFVERVSKR
                     VPVFVDEAYIDYLPDPQGTTLIPMANSNPNIIVARTFSKLYGFAGLRLGYVVTQPDII
                     KKLSLYTEASMSITATTLAAALATYQDREFLDAALKKTVASKEYLYEVLKKEGYAYVP
                     SSTNFVLFPIKMEGKQFTEEMMKRGVGVRFWKFNNKEWCRVSIGRMDEMEAFAAAFKE
                     IS"
     gene            3483..>4084
                     /locus_tag="VKQ52_04545"
     CDS             3483..>4084
                     /locus_tag="VKQ52_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86478.1"
                     /translation="MKKIILSVCLLAAGVFAVRAQDSAASRALTMTEYEKAKTFSVSD
                     PDKDTYVKFDNAYILDHSGFGKPYFITGDDGKKKRIDLYKLILKDGRVELGTIIYYTT
                     ENGKRYTACLPGYKADGKVWEKYFEDIHAIDKQEQFYVLKLSYVLSKELGFQQYRNAT
                     GALGKEVSREAGTYGNDICFPGDMEVAMAGGARKPLCEVKA"
BASE COUNT          945 a          892 c         1290 g          957 t
ORIGIN      
        1 cgggtgtcaa tggctatgga ggcaatgact tcccggtgat gcggtatgcg gatgtgatcc
       61 tgatgctggc ggaggtgagt atgtaccagg gaaatgcggc gcaggctatc cagtacctga
      121 atattgtgcg gacgcgggct aagatgccgg attatgcgac ttcgatgttg aatcccgcct
      181 atgccgcgaa attcccgacg ctgaagctgg ccattcttca tgagcggcga agcgagctgg
      241 cttttgagca tcaccggctt ttcgatctgt tgcgcttctt caatgtcaac gacctggtga
      301 cgtatatcca ttcgaagaac gcggcggact ggggtcttgc gaatattgcc aatttcggta
      361 ccaaggatga atactatccc ataccgtaca atgaggtgat actggatccc aagcggatgt
      421 accagaaccc ggggtattaa agctaattcg atggaggagg caaaaccgcg gcgatggtcg
      481 cggttttttc tttatattta tttgcagggg cggaggttga aaatttggga aaagcgacat
      541 taagcgcgga tgatacaaat tagtttttat ttaatattta taagaagagg tccgaatgcg
      601 cgtttttttg tatttttaaa tagaattttt aatatttcat ttttttagta tttgaactct
      661 ttaacacgca gggaatttct taggagaacg ggtgttttgg ccggtggagc ttatccggcg
      721 atgatggcga tggggatggt accggctgca ccggtgcgtc ctttccggtt ggaggggagt
      781 ggtgggggta aggaagtggt tatcctcggg gccgggctgg ccgggatggc ggcggcgtat
      841 gagctgggta agcttgggta tcattgtacg atactggagg cccgggagcg ggcgggtgga
      901 cggtgctgga gtatccgcaa gggctcggtg aatgaggaga cgggaggcgg atcgcatacg
      961 gccggttttg atgagggtca gtattttaat gccggtccgt cgcgaatccc gcatcatcac
     1021 gggcttaccc ttcattactg caaggaactg aatgtgccgg tacaggtata taacaatgtg
     1081 aacgagaaca cctattacta tagtgagggg aaggggccgt tgtccaataa gaaggtgagg
     1141 gtacgggagg tgcataacga tatgcggggt tggatggcgg agctgttggc gaaggggatg
     1201 gatgggggga agatcgatgc cgggatgagc agggaggata cgctaaaggt gatagaatat
     1261 ttacgggccg agggcgggtt ggatatcgac aagctgtata aggcatcggc ccgccggggg
     1321 tatatcgagt cgccgggtcc cggggaccgg gaagggaagc tgggggatcc gtcgaccctg
     1381 ctggcgatgt tgcagtcggg tttgctggac cctgattttt ataatgtggc cgaatacacg
     1441 tatgagttgc aggggacgct tttccaggcg gtgggtggaa tggacaggat cgcggccgct
     1501 tttgagcaac gggtggggaa tctgatccgg tatcaggcgg aggtgagcaa gatccataac
     1561 ctggaacagg gggtggaggt aggctataaa gacaagacgg gtgaaaagac aataaaagcc
     1621 gaattttgta tctgcacgat cccgttgccg gtgttgagca atatcgatca caactttgct
     1681 tcggatgcca gccgggccat cgattccatc gtgtatatgt cgaccgggaa gatcggactg
     1741 cagtttaagc gccggttctg ggaggaggat gagggcatct acgggggtat cacccacacg
     1801 aacaatcagt taacgcagat attctatccc tcctatgatt atttgagcgg taagggaatt
     1861 ttgatcggat attataactt taatgagaag gctgtagcca cgggcaatct ttcttatgcc
     1921 caacgggagc aactggcatt ggacaagggc agcctgatcc atccgcagta caggcaggag
     1981 ttcgagacgt cgttttcggt gagctggcac aagaccaaat acaatctggg cggatgggcg
     2041 ctgtactcgg cggaggagcg gcagacgttg tacaaggctt tgctgcagcc ggacaagcag
     2101 gtgtattttg ccggggagca tatgacctat ctgaatgcgt ggatggcggg tgcgctggag
     2161 tcggcgcgga gtgttgtggc ggctttgcat agccgggtga cgggtcagca tttttcgtac
     2221 ccgggctttg gaagttgatc cggggcggac ggttgggaat acgtatttaa ccaccaaaaa
     2281 tatactctta tgggcaactc aatcagtcgt cgcacccttc ttaagagggg cgctctattg
     2341 gccggcggtc ttccttttgc cgccagcctg ctggataaag tgcaggccag tccattgatc
     2401 gtaaaacccg gagaatctgc cgttccgtat acggaagcct ggacggaaaa acaaattgcc
     2461 ctgaatgccc cggcgccgtt gaaggcccgg ctttttgcca atgaaaatcc ctttggtccg
     2521 tccgacaagg caaaaaaagc catcatggaa gcctgtccga ccagctatca atatcctttc
     2581 cgggcgatgc ccgatctgat caagaagatc gccgccttcg agggcgtgaa ggaagagaat
     2641 atcctgctgg cctccggttc atcgcccttg ctgatggcgt cggccttata tttcagcaag
     2701 cccggcggca atatcatcac cggtgatccg tcgtatgagg atctgcccag caaggcggtg
     2761 cggatgggag agaagtgggt caaggtgcct ttgacggccg atttcaagtt ggacctggtg
     2821 gcgatggaaa aggccatcga tgagaatacc gggctggtct atgtctgcaa tcccaacaat
     2881 ccgacggcca cggtgctgga tacggcgcaa ttgaaagcct ttgtcgaaag ggtgtccaag
     2941 cgggtgccgg tgtttgtcga tgaagcctat atcgactatc tgcccgatcc gcagggaact
     3001 acgctgatcc cgatggccaa ttcgaatccc aatatcattg tggccaggac cttctccaag
     3061 ctctacggct ttgcgggtct ccggcttggc tacgtggtga cgcagcccga tatcatcaag
     3121 aaattatcgc tttatacgga ggcttccatg tcgatcacgg cgactacgct ggcggcggcg
     3181 cttgccacgt accaggaccg ggagttcctc gatgccgcgt tgaagaagac cgttgcatcc
     3241 aaggaatatc tctatgaggt gttgaagaag gaagggtatg cctatgttcc ttcgagcacg
     3301 aacttcgtcc tcttccccat caagatggaa ggcaagcagt ttaccgagga aatgatgaag
     3361 cgtggggtgg gtgtgcgttt ctggaaattc aataataagg aatggtgccg ggtcagtatc
     3421 ggtcgcatgg atgaaatgga ggctttcgcc gccgcattta aagaaataag ctaaattcag
     3481 gaatgaagaa gatcatttta tcggtctgcc tactggcggc cggcgttttt gccgtgcgtg
     3541 cccaggattc ggcggccagc cgcgccctta cgatgacgga gtacgaaaag gccaagactt
     3601 tttccgtgag tgatcctgac aaggacacct atgtcaaatt cgataatgcc tatatcctgg
     3661 atcacagtgg ttttggcaaa ccctatttta tcacggggga tgacggcaag aagaagcgga
     3721 tcgatctgta caagctgatc ctcaaggatg ggcgggtcga gctgggcacc atcatctact
     3781 ataccacgga gaacggcaag cgttatacgg cctgtctgcc gggctataag gcggatggga
     3841 aggtatggga gaagtatttc gaggatatcc atgccatcga caagcaggag cagttctatg
     3901 ttctcaagct gtcgtatgtt ttgtcgaagg agctgggttt ccagcaatac cggaatgcca
     3961 cgggggcgct ggggaaggaa gtgagccggg aggccggtac gtacggcaat gatatctgtt
     4021 tccccgggga tatggaagtg gcgatggcgg gtggtgccag gaaaccgctt tgcgaggtga
     4081 aggc
//
LOCUS       DATGFG010000245         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_382179, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000245 DATGFG010000000
VERSION     DATGFG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382179"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..464)
                     /locus_tag="VKQ52_04550"
     CDS             complement(222..464)
                     /locus_tag="VKQ52_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86479.1"
                     /translation="MTITDWTGAVGVTTLLVAFLLNLLGKIEKEGFAYLLLNVAGAGI
                     ACLASVQLRYLPFIILEGCWTLVSAVGLVAHVKSRR"
     gene            552..1028
                     /locus_tag="VKQ52_04555"
     CDS             552..1028
                     /locus_tag="VKQ52_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLZ86480.1"
                     /translation="MRKTLLPILLLVLLFARCQNDTAARDTTTATTPPPATAIADTSR
                     LIGVMQKSAEDWNKGDLAGFMDSYDDSATMMSRHGLINKDSMMAHYQQTYFSKGAARQ
                     QLTFDQLQITPLAGDYVLLTGRFTLDGNQLPTQSGHFSLVCVHRKKGWKILHDHTS"
     gene            complement(1101..1496)
                     /locus_tag="VKQ52_04560"
     CDS             complement(1101..1496)
                     /locus_tag="VKQ52_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ86481.1"
                     /translation="MAASSKTFFAPQLIIKSGVMELDFYQNAFGAVELRRFSNDDGSI
                     HVSEMSIDGALFHFHEERPAAGSVTPGQAGGVTTIIGLMVADVDAVMERAKAAGGLVK
                     SPAQSYDYGYRQGQIIDPFGHHWLIEMVI"
     gene            complement(1548..2051)
                     /locus_tag="VKQ52_04565"
     CDS             complement(1548..2051)
                     /locus_tag="VKQ52_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86482.1"
                     /translation="MKRAFLFVALPFFVLAACKKNNTSSAATGISATINGKKVIFNGI
                     TRIDTSGGNLYISCSVDSLTFGSPLITLFVQGTRPLKTGLYPNLSLAPRASFQSDLSY
                     ATYQGYTSQQYQSYDDSVTVSFVNNSSASGTFSGRALYSDVDPGDAGRHEDRILTITD
                     GKFNVRW"
     gene            2275..2712
                     /locus_tag="VKQ52_04570"
     CDS             2275..2712
                     /locus_tag="VKQ52_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86483.1"
                     /translation="MLHAKDLRFGNKVFNQQGEVITVQQILSNSVVYDTSLSVNRQTS
                     KVSNGYNYSYTMQVVEVVKEAELQEMEPIRLTPKILEKCGFRNYVREEWIITYGNTHT
                     DFEFTEDGLKLRHATPARVGIKYVHQLQNFFYAITGHELDVDL"
     gene            complement(2800..3165)
                     /locus_tag="VKQ52_04575"
     CDS             complement(2800..3165)
                     /locus_tag="VKQ52_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HLZ86484.1"
                     /translation="MAKAIKKAAKKAVKKAAKKAPAKAAKKAPKKAAKKASTRKPNAA
                     FMTPLSPSATLAEVIGSKPLPRTEIVKKIWDYIKKNNLQDKKNKRMINADTKLKAVFG
                     GKDQVSMFDLAKIVSKNVK"
     gene            3339..>5281
                     /locus_tag="VKQ52_04580"
     CDS             3339..>5281
                     /locus_tag="VKQ52_04580"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HLZ86485.1"
                     /translation="MLAIRLLSGIGALLISACCLASDLDLSSPDGNIRITWHPTDSFP
                     CYSVRYKGVILVDQGPLDLVFREPGEARPASFFGPGMLIRPAYREGTDAYTLPVGRNS
                     RVNDRYNEYAVSMEQPRFRRRRLTFILRAYNDGIAFRWVFPKQPDWNAYELFAENTGI
                     RLAGNPWLQASLLPGYTTSHEGRYTRLPFSEVKSDTLMDTPVFLEFPGGNSNREPSNR
                     EPLYMAITEAQLTDYAGMYLIKHNGILKSELSPLPEGHGIRVKAILPHRTPWRVLFIG
                     ARPGAFLESNLLTDLNDSCAIKDLSWLKPGKTDFHWWNGDIVPDTSFPPGLNFETNQY
                     YIDFCARNGLAYHTVIGYGGTAWYRNDGVSYAPGPHSDVTQPVPGLDMKQLCDYAAAK
                     GVGIRVWVHWQVLCRNLDKAFTQFEKWGIKGMMVDFMDRDDQGMVNIQTEILRKAAEH
                     HLHIQFHGAYKPTGLIRTWPNELTREGTLNYENDKWDNPITPEDDLNVVFTRLLAGAT
                     DYHLGGFRAVPRSGYTAQYTRPLVLGTRCHMLAMYVVLESYLPMVCDYPAAYEGQDGF
                     DFIKAVPSTWDETRVPTALPGQWAAIARRSGDDWWIGTINGPIARTLTIPLGFLQPGR
                     YAATVYSDAPDAAGNPNHLVRTTL"
BASE COUNT         1241 a         1631 c         1306 g         1103 t
ORIGIN      
        1 cctcctcacc cgcctgcgcg ccctcctcgc cgaggacacg acgctctccc ggcgcttcgt
       61 cgatctcaac aggaacgact tcaaccctgc cgaactacaa gaggaaaacg atctccgcac
      121 cgcccgtatc cgcgaactct acaaccgcct gggcgggcgg cgatgacggg caaaaggccg
      181 tccaggagct tcctgcacgc cgcgcaaagt cctcgcaccg ctcagcgtct gctctttaca
      241 tgcgccacca accccactgc cgacaccagg gtccagcagc cctcgaggat gataaagggc
      301 aaataccgca attgcaccga tgcaagacag gcgatcccag ccccggcaac attcaggagg
      361 agataggcaa acccctcttt ttctatctta cccaataaat tcaggagaaa ggcgaccagc
      421 agggtggtca cgccgacggc tccggtccag tctgttatcg tcatagccgc aacttacgac
      481 aattggggca tcatcggcaa aagccgaata tgccggcgag tcaggggctt tttttgctat
      541 ttttacgggt aatgagaaaa acgctcctcc ccatcctgct cctggtgctc ctttttgccc
      601 gctgccagaa tgatactgcc gcccgcgata ccaccacggc aacgacaccc ccgccggcaa
      661 ccgccatcgc ggataccagc cggctcatcg gcgtcatgca aaaatctgcg gaagactgga
      721 acaaaggcga tcttgccggc ttcatggact cctacgacga tagcgccacc atgatgtccc
      781 gccacggcct gatcaataag gattcgatga tggcccatta ccagcagacc tatttcagca
      841 agggcgccgc gcggcaacaa ctgaccttcg accagttgca gataacaccc ctcgccgggg
      901 actatgtcct cctcaccggc cggttcacgc tggatgggaa ccagctgcca acccagtcgg
      961 gccatttctc cctcgtttgt gtacaccgga aaaagggctg gaagatcctg cacgatcaca
     1021 ccagctaaag tcgggaacca gccgacggcg ggcaacagct gatggcgggg accacctgat
     1081 ggcgggcaac agccgacggg ctaaatcacc atctctatca gccagtgatg cccgaacggg
     1141 tcgatgatct gcccctgccg gtatccataa tcatagctct gcgccgggga tttcaccaag
     1201 cctccggcag ccttcgcgcg ctccatcacg gcatccacgt cggcaaccat cagcccgata
     1261 atggtcgtca ccccgcccgc ctgcccggga gtgacactgc ccgcggccgg gcgttcctca
     1321 tgaaaatgga aaagcgcccc gtcgatcgac atctccgaga catggatgct tccatcgtca
     1381 ttgctgaagc gtcggagctc tacggcgcca aaggcgtttt ggtaaaaatc aagctccatc
     1441 accccgcttt tgatgatcag ctgaggggca aaaaaggttt tggaggacgc tgccatggat
     1501 gtacgttttg ggtaacctaa acatacaaaa agcccttcaa acgcgcatca ccaccgcaca
     1561 ttaaacttcc cgtcggtgat cgtaaggatc ctgtcctcat gcctgccggc gtcgccggga
     1621 tcgacatcgc tatagagggc cctgccactg aaggtgccgc ttgccgagga attgttgaca
     1681 aagctgaccg tcaccgaatc atcatagctc tggtactgct gcgaagtata accctggtag
     1741 gtggcatagc tcagatcact ctggaaggag gcccgcggcg ccagggacag attggggtag
     1801 aggccggtct tcagcggccg tgtcccctgg acaaacaggg tgatcagggg ggaaccgaat
     1861 gtaagggagt cgaccgagca ggagatataa agattgccgc cgctggtgtc gatacgcgta
     1921 atcccgttaa agatcacctt cttcccattg atggtggcgg aaatgccggt ggcagccgaa
     1981 gaggtattat tctttttgca ggccgccaat acaaagaaag gaagggccac gaaaagaaag
     2041 gctcttttca tgtgatattg aatttgcatc gaaacgaaga gtccgcaaaa aaaattgtaa
     2101 acccacggcg tatttttacg aaccctcgcc aagcgcgcat ctttttttga ccaaaaggtt
     2161 gcaaatttcg gaacaggcta gtatttttat cccgataatc cgcagttgat agagtctatt
     2221 cccttgaact accatgagac aattcattgt atttcaaatg taaaacgaga ctctatgtta
     2281 cacgcaaaag atcttcgatt cgggaacaaa gtttttaatc aacagggcga agtcattacc
     2341 gtccagcaga tcctctccaa cagcgtcgta tacgacacct ctctcagcgt aaacagacaa
     2401 acatccaaag tgagcaacgg ctataactat tcctatacca tgcaggtggt ggaagtcgta
     2461 aaggaggccg agttgcagga aatggaaccc atccggctga cgccaaagat cctggaaaaa
     2521 tgcggcttcc gcaattatgt ccgcgaggaa tggatcatca cttacggcaa tacgcacacg
     2581 gactttgaat ttaccgagga cgggctgaaa ctgagacatg cgacacctgc ccgcgtaggc
     2641 atcaaatacg tacaccagct gcagaatttc ttctatgcca tcaccggcca tgagctggat
     2701 gtcgacctgt aaaaaaagcc gtcgaaaaag aaccgtcgaa aaaaaagaca taaaaaaacc
     2761 tgccatcggc tgatggcagg ttgaatgtca ggagacaact tacttcacgt tcttgctcac
     2821 gatcttggcc aggtcgaaca tcgaaacctg atcttttccg ccgaagacag cctttaactt
     2881 ggtatcggca ttgatcatgc gcttgttctt cttatcctgc aggttgttct tcttgatata
     2941 atcccatatc ttctttacga tctccgtcct gggcagaggc ttgctgccga tcacttcagc
     3001 cagcgttgcg ctgggagaga ggggagtcat aaatgccgcg ttgggcttgc gagtagaagc
     3061 tttttttgcc gcttttttgg gcgctttttt cgctgctttt gcaggtgcct ttttagcggc
     3121 tttcttgact gctttcttcg cagccttctt gatagctttt gccatgatta tttcatttta
     3181 atgggttaat gaaataaaag tacagatttt aatttttctg aatgcataat ttttaaaaat
     3241 ttcgacgggc ggcgatgaaa ttcccccgac ggcgcgcctg ccccggcgca gcggaacgat
     3301 aattcccata cgcttacctt tacaaaaaat tgcaatcgat gcttgctatc cgcctcctct
     3361 ccggtatcgg cgccctcctg atttccgcct gctgcctcgc aagcgacctc gacctctcct
     3421 cccccgacgg caatatccgg atcacctggc accccacgga tagcttcccc tgttatagtg
     3481 tccggtataa gggggtaata ctcgtcgacc agggccccct cgacctcgtc ttcagggaac
     3541 ccggagaagc ccgccccgcc tcgttcttcg gccccggcat gctgatccgg cctgcctaca
     3601 gggaaggcac ggacgcctat acgctgccgg tcggccgcaa cagccgggtc aacgaccgct
     3661 ataacgaata cgccgtctcg atggaacagc cccgcttccg ccgtcggcgg ctcaccttta
     3721 tcctcagagc atataacgat gggatcgcct ttcgctgggt ctttccaaaa caacccgact
     3781 ggaacgcata cgaacttttc gccgagaaca ccggtatccg gctcgccggt aacccctggc
     3841 tccaggcctc cctcctcccc ggctatacca cctcccacga gggacgctat acccgcctgc
     3901 ccttcagcga ggtgaaaagc gataccctca tggatacacc cgtcttcctc gaattccccg
     3961 gcggcaactc caaccgcgaa ccctccaacc gcgaacccct atacatggcc atcaccgaag
     4021 cgcagttgac cgactacgcc ggtatgtatc tcataaaaca caacggtatc ctgaaaagcg
     4081 agttatcccc cctgccggag ggtcatggaa tacgggtaaa agccatcctc ccgcaccgga
     4141 caccctggcg cgtactcttc atcggggcac gccccggcgc ctttctggaa tccaacctgc
     4201 tcaccgacct gaacgactcc tgcgcaataa aggacctctc ctggctgaag ccgggcaaga
     4261 cggattttca ctggtggaac ggcgacatcg tccccgacac cagcttccct ccgggcctca
     4321 atttcgagac aaaccaatat tatatcgatt tctgcgcccg caatggcctg gcctatcata
     4381 cggtcatcgg ctatggcggc accgcctggt acaggaacga tggcgtcagc tatgccccgg
     4441 gcccccattc cgatgtcacc caacccgtcc ccggtctgga catgaaacag ctctgtgatt
     4501 atgccgctgc aaaaggtgtt ggcatccgtg tctgggtcca ttggcaggtg ctctgtcgca
     4561 acctcgacaa ggccttcacg cagtttgaaa aatgggggat aaaaggcatg atggtggatt
     4621 tcatggatcg cgacgaccag gggatggtca atatccagac ggagatcctg cgaaaggcgg
     4681 ccgaacatca tctgcatatc cagttccacg gcgcctataa gcccacgggc ctgatccgca
     4741 cctggccaaa cgaactcacc cgcgaaggaa cactcaacta cgagaatgat aaatgggaca
     4801 accccatcac ccccgaagac gatctcaacg tcgtctttac ccgcctgctc gccggggcca
     4861 ccgattacca cctcggagga ttccgggccg tgccccgctc cggctatacc gcgcaatata
     4921 cccggcccct cgtgcttggc acccgctgcc atatgctcgc catgtatgtg gtgctggaaa
     4981 gctatctgcc gatggtctgc gattaccccg ccgcctatga agggcaggat ggattcgatt
     5041 tcataaaagc cgttccctcc acctgggatg aaacccgtgt tccgacagca ttgcccggtc
     5101 agtgggctgc cattgcccgc cggagcggcg acgattggtg gatcggaacg atcaacggac
     5161 ccatcgcccg cactctcacc atcccgcttg gtttcctcca gcccggccgc tatgccgcaa
     5221 ccgtctatag cgatgcaccc gacgcagccg gaaaccccaa ccatctcgtc aggacaaccc
     5281 t
//
LOCUS       DATGFG010000246         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_107955, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000246 DATGFG010000000
VERSION     DATGFG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107955"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VKQ52_04585"
     CDS             <1..424
                     /locus_tag="VKQ52_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HLZ86486.1"
                     /translation="DYGLEFYDAHTPNAMYSLQMGARGMSSISGNFYPEILVWMCNNA
                     NDPDRQEDVRWLQGELSRVDPLIHIAYPMSAKYFLQKRGLPVRTISRAHALELTVQQK
                     QTLDDIYKSFLGWCERLDIQPVQTGPLVRPKMPLDPPI"
     gene            431..1558
                     /locus_tag="VKQ52_04590"
     CDS             431..1558
                     /locus_tag="VKQ52_04590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLZ86487.1"
                     /translation="MSTLRIHFPGKLVFGNHCIDQLPDEVTTLAAARVFIVTITPLLT
                     PLQPLIHQLEKKGIHIAVYTGITQEPSFEDFNQVMAEAGPFDPDLVLGIGGGSVLDVA
                     KLVAAQLGNDQSLSDYVGIGLLKGRKRKLICMPATSGTGSEVSPNAILVDNADNQKKG
                     IISPFLVPDIVFVDPLLTISVPPAITAATGLDALTHCLEAYTNTFAQPFIDMYAFEGM
                     RLIAANLVRAVRNGADEEARSQVAMGSLLGGFCLGPVNTAAVHALSYPLGSSFHLPHG
                     LSNALLLPYVMEFNLPATPGRYAGVARALGANTAEEGITKVRQLIRDCGIPATLKEVG
                     VPFDAIETMAADAMKITRLLKNNPRPVTIDDAISIYKAAYE"
     gene            1551..2375
                     /locus_tag="VKQ52_04595"
     CDS             1551..2375
                     /locus_tag="VKQ52_04595"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HLZ86488.1"
                     /translation="MSKKYHGIVVPAITPLNADLSLDVASVERLLPLFPHPFILGTTG
                     EAPSLPTRLKEQYIRLAARIKKPGQQLYVGISSNCFADSVEYAKIAFGEGADVTVATL
                     PSYYPLSEGQMRSYFLRLADTLSGALIIYNIPSTTHHSIPLALIDELSHHPRIVGTKD
                     SERNEERLKESLQRWSARQDFSHFLGWAAKSGFTLLNGGDGLVPSTANLAPGLYSDME
                     KAAAAGNKDEIARLQEMSDCLGNSYQSDRSLGESLAMLKLIMQNQGLCQAYMMPPL"
     gene            2386..>3393
                     /gene="pdxA"
                     /locus_tag="VKQ52_04600"
     CDS             2386..>3393
                     /gene="pdxA"
                     /locus_tag="VKQ52_04600"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLZ86489.1"
                     /translation="MSNKPIIGITMGDPASIGPEIAIKALLTEKVTSICRPLIVGDAA
                     TLDYTIDHLKWKAKVHPITKVSEALFQPGILDVLDMETVDLDTLVWGEISAMAGHAAF
                     EAVRKVIELAMAGEIDATVTGPINKKSINDAGHHFAGHTEIYAHYTGTAKYGMLLVED
                     NLKVIHVSTHVSLRQACDLVKKDRILAVIALLQDGLIRLGEKNLKIGVAGLNPHAGDS
                     GLFGTEDDQEILPAVLEARKLGYDVDGPVPPDTLFAKAATGHYGGVVAMYHDQGHIPF
                     KLSGFKWNAEKQRMDSVKGVNITMGLPIIRTSVDHGTAFEIAGKGIASADAMILAIEA
                     AV"
BASE COUNT          784 a         1117 c          836 g          656 t
ORIGIN      
        1 cgactatggt ctcgaattct atgatgcgca tactcccaac gccatgtatt ccctccagat
       61 gggcgccagg gggatgtcat ccatctccgg gaatttctac cccgagatcc tcgtctggat
      121 gtgtaacaac gccaacgacc ccgatcgcca ggaagacgtc cgctggctgc aaggggaact
      181 aagtagagta gacccgctca tccacatcgc ctacccgatg agcgccaaat atttcctcca
      241 gaaaagagga ttgcccgtcc gcacaatcag ccgcgcccat gccctcgaac tgacggtcca
      301 gcaaaagcag acgctcgatg atatctataa aagctttctc ggttggtgcg aaagactgga
      361 tatccaaccg gtgcagaccg gccccctggt ccgcccaaaa atgccactcg atccccccat
      421 ttgataaacc atgtcgacac tacgcataca ttttcccgga aaactggtct tcggcaacca
      481 ctgcatcgat cagctgcctg atgaggtcac aacccttgcc gctgcacggg tattcattgt
      541 caccattact cccttactta ccccattaca acccctgatc caccaactcg aaaaaaaagg
      601 catccacatc gccgtataca cggggatcac ccaggagccg tcttttgagg actttaacca
      661 ggtcatggcg gaggccggcc ctttcgatcc cgatctcgta ctgggcatcg gcggtggcag
      721 cgtgctcgat gtcgccaaac tggtggcggc ccaactgggc aacgaccagt ccctttccga
      781 ctatgtcggc atcggcctgc taaaaggcag aaagcggaaa ctcatctgca tgccggccac
      841 ctccggcaca ggcagcgaag tttcgccaaa cgccatcctc gtggacaacg ccgacaacca
      901 gaaaaaaggg atcatcagcc cgtttctcgt tccggatatc gtgttcgtcg atccgctgct
      961 gaccatcagc gtcccgccgg ccataaccgc cgccacaggc ctggatgcgc ttacccattg
     1021 cctcgaagcc tatacgaata cctttgccca accttttatc gatatgtatg ccttcgaagg
     1081 catgcgcctc atagcagcta acctggtccg tgccgtccgc aacggcgccg acgaagaggc
     1141 cagatcacag gtagcgatgg gcagcctcct gggtgggttc tgtctcggcc cggtgaatac
     1201 cgctgccgtc cacgcccttt cctatccgct gggtagcagc ttccaccttc cgcatggcct
     1261 gtccaatgcc ctcctgctgc cctatgtcat ggaatttaac ctccccgcta cgcccggtcg
     1321 ctatgctggc gtggcccgcg ccctgggtgc aaacacggcc gaagagggta taacgaaggt
     1381 tcgccaactc atccgcgact gcggcattcc cgcaaccctg aaagaagtag gagttccctt
     1441 tgacgccatc gaaaccatgg cggccgacgc catgaagatc acgaggctcc tgaaaaataa
     1501 cccccggccc gtcactatcg acgacgctat ctccatttat aaagccgctt atgagtaaaa
     1561 aataccatgg gattgtcgtg ccggcgatca ctccactgaa tgccgatctt tcgctggacg
     1621 tcgcgtccgt ggaaaggctc ctgccgctct tccctcatcc ctttatcctc gggacaacgg
     1681 gggaagcccc ttcattaccc acccggctga aagaacaata cattcgcctt gccgccagga
     1741 taaagaaacc cggccagcaa ctctacgtcg gcatctcctc caattgcttc gccgattccg
     1801 tagaatatgc gaagatcgcc ttcggcgaag gcgccgacgt cacggtggct accctgccat
     1861 cctactatcc gctttccgaa ggccagatgc gcagttattt cctacgcctt gccgataccc
     1921 tctccggtgc cctcatcatc tacaatatcc cttcgacaac ccatcattcg atacccctcg
     1981 cgctgatcga tgagctcagc catcacccgc ggatcgtcgg gacaaaggac tccgaacgca
     2041 atgaagaacg cctgaaggag tccctgcaac gctggtcggc ccgacaggat ttcagtcatt
     2101 ttcttggctg ggcggcgaag tccggcttca cactgctcaa cggcggcgat gggctcgtac
     2161 ccagcacggc caacctggcg cccggcctgt acagcgacat ggagaaggcg gcagccgccg
     2221 gcaacaagga cgaaatagca cgcctgcagg agatgtccga ttgcctcggc aactcctatc
     2281 aatccgaccg gtcgctcggc gaatccctgg ccatgttaaa actcatcatg caaaatcaag
     2341 gtctctgtca ggcatatatg atgccgccct tataaaacgc agaaaatgtc aaacaaaccg
     2401 atcataggaa tcaccatggg cgacccggcc agcatcggcc cggaaatagc gatcaaagcc
     2461 ctgctgacgg aaaaagtcac gtccatctgc cgccccctca tcgtcggcga tgccgccacc
     2521 ctcgactata ccatcgacca cctgaaatgg aaagcaaaag tccatcccat tacgaaagta
     2581 tccgaagccc tcttccaacc cggtatcctc gacgtgctgg atatggagac cgtcgatctc
     2641 gataccctgg tctggggcga gatctccgcc atggcaggcc acgccgcttt cgaagccgtc
     2701 cgtaaggtca tcgaactggc catggccggg gagatcgacg ccaccgttac cggaccgata
     2761 aacaaaaagt ccatcaacga cgcggggcat cacttcgccg gtcacacgga gatctatgca
     2821 cactataccg gcaccgcaaa atatggaatg ctgctcgtcg aggacaatct gaaagtcatc
     2881 catgtctcga cccatgtctc ccttcgacag gcctgcgacc tcgtgaaaaa ggaccggatc
     2941 cttgccgtca tcgccctcct gcaggacggc ctcatccgcc tcggcgaaaa gaatctgaag
     3001 atcggcgtgg ccgggctgaa cccccatgcc ggtgactccg ggctcttcgg tacggaggat
     3061 gaccaggaga tcctgccggc cgtcctcgag gccagaaaac tcggatatga cgtcgacggg
     3121 cccgtgcctc ccgatactct tttcgcaaag gcggccacgg gccattacgg cggcgtcgtt
     3181 gcgatgtacc atgaccaggg acatatccct ttcaaactct ccggctttaa atggaacgcg
     3241 gaaaaacaac gcatggatag cgtcaaaggg gtcaatatca ccatgggcct gcccatcatc
     3301 cgcaccagcg tcgatcacgg caccgccttc gagatcgccg gcaaaggcat cgccagcgcc
     3361 gacgccatga tcctggccat cgaagccgcc gta
//
LOCUS       DATGFG010000247         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_267582, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000247 DATGFG010000000
VERSION     DATGFG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267582"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VKQ52_04605"
     CDS             complement(<1..990)
                     /locus_tag="VKQ52_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ86490.1"
                     /translation="MRILLKYLKPYRWLVMLALGLAAINQVFSMLDPYFMGKLGLDKL
                     AGHPWDTGYFDDRKVFHKTGVRTQSQFIWAAIAFLGIQVSVAMVSRIAKAFQDYFSNV
                     IVQKFGATIFTNGLRHSMALPFSEFEDQRSGETLSILTKVRADTEKFIISFINVLFTI
                     IVGVIFVSIYSFTLHWSIMPIYVTGMILLSLLTSILSRKIKRIQKTITTETNALAGST
                     TESLRNIELVKSLGLTEQEVKRLNQNTFKILGLELRKVKSIRALSFVQGTFVNFLRQC
                     IMFLLLWLVFGHRLTLGEVMTMVFYSFFIFGPLQELGNIILTYREAEASLNNFD"
     gene            complement(1043..2323)
                     /locus_tag="VKQ52_04610"
     CDS             complement(1043..2323)
                     /locus_tag="VKQ52_04610"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLZ86491.1"
                     /translation="MSRKHLYLYLTFAVLATTVFSWFRINSLCIILLAAYKLWENKPL
                     PAVKAAFTNKFVIAYLVFFLVEAVGLLHTHNMKAGGDALSKDATLVAIAFTLCAGTFV
                     DERGYKQVMTGYCGILLAASLYCLVVATRNYLSTRDIAVFFYHPLTACISQNAVFYSV
                     YVLFGALYLLTPGGILILPESWKAVRRPLQICLVVFFGGMIVLLSSKLILVIFLIIVI
                     VSLTKNYSARRDWKLLLVVAIIAFALTGVLAVTNNPIKSRFQELMAGDLELVKKDRFA
                     PGDSFNALQIRMLELRFAWAILNEQHAWLFGVTPGDSQDLLDRKYINANMYIGNPADG
                     PHRKIRGFIGYNFHNQYVETLVRDGILGLAALLAIFWLLAGMVWKWKTRLAFFTVLTL
                     FVFFIPEAPLTMQQGIFLFCFFPLFLLHSPKRTD"
BASE COUNT          600 a          574 c          692 g          485 t
ORIGIN      
        1 gtcgaaatta ttgagcgatg cctccgcttc gcggtacgtg aggatgatat tgccgagctc
       61 ctgcaagggg ccgaatataa aaaaggaata gaagaccatg gtcatcactt ccccgagggt
      121 aagccggtgt ccgaagacca gccagaggag gagaaacatg atacactggc ggaggaaatt
      181 gacgaatgtc ccctgcacga agctgagggc gcggatgctt tttacctttc gcagctccag
      241 gcccaggatc ttgaaggtat tctggttgag gcgtttcact tcctgttcgg tgagcccgag
      301 gcttttcacc agttcgatat tccggaggga ctcggtggtg ctccccgcca gggcgttggt
      361 ctccgtcgta atggtcttct ggatgcgttt gatcttcctg ctgaggatgc tggtcagcag
      421 ggatagcagg atcattcccg tcacatagat cggcatgatc gaccagtgga gcgtgaagga
      481 gtagatggat acgaagatga cgccgacgat gatggtgaaa aggacattga taaaagaaat
      541 gatgaacttt tcggtatcgg cgcgcacctt ggtcaggatg gagagggtct cgccgctgcg
      601 ctggtcctcg aattcactga aggggagggc catggaatgg cggaggccat tggtaaagat
      661 cgtggccccg aatttctgga cgatgacatt gctgaaatag tcctggaagg ccttggcgat
      721 ccgggagacc atggccacgc tgacctggat acccaggaag gcgatggcgg cccagatgaa
      781 ctgggattgg gtccggacgc cggtcttatg aaagaccttt cgatcgtcga aatagccggt
      841 atcccagggg tgacctgcca gtttatccag ccccagtttc cccataaaat agggatcgag
      901 catggaaaag acctggttta tggctgccag accaagcgcc agcatgacca gccaacggta
      961 gggttttagg tattttaaga gaattctcat tggagtacaa tttcggaaaa aaacaacaag
     1021 tgaaccgtca ttctgttctg tactaatccg ttctcttcgg actatggaga agaaaaaggg
     1081 ggaagaagca gaacaggaag atgccttgct gcattgtcag gggagcttcg gggataaaaa
     1141 agacaaaaag cgtcagaacg gtgaaaaaag caaggcgggt cttccatttc cagaccatgc
     1201 cggccaggag ccagaaaatg gccaataagg ccgcaagtcc caggatcccg tcccgcacaa
     1261 gcgtttcaac atattggtta tgaaaattgt agccgataaa accccggatc ttccgatgcg
     1321 gcccgtcggc ggggttgccg atatacatat tggcgttgat atacttccga tccagcaggt
     1381 cctggctgtc gccgggtgtc accccgaaga gccaggcatg ttgctcattc aggatggccc
     1441 aggcgaaccg cagctccagc atcctgatct gtaaggcatt aaaggaatcg cccggcgcaa
     1501 aacgatcctt tttcaccagt tcgaggtctc cggccatcag ctcctggaac cggcttttga
     1561 tcgggttgtt ggtgacggcg aggactcccg tcaaggcaaa cgcgatgatc gccaccacga
     1621 gcagcagctt ccaatccctt cgcgcggagt aattctttgt caggctgacg atgacgatga
     1681 tcaggaagat caccaggatg agtttggagg ataaaaggac gatcataccg ccgaaaaaaa
     1741 cgacgaggca gatctgcagg ggtcttctaa cggccttcca gctttccgga aggataagga
     1801 tgccgcccgg cgtcagcagg tataaggcgc cgaagaggac ataaacggaa tagaagaccg
     1861 cattctggga gatacaggcg gtgagcggat gatagaagaa gacggcgata tccctggtgg
     1921 aaagataatt ccgggtcgca acgacaaggc aatatagcga agccgccagc aggatgccgc
     1981 aatagccggt catcacctgt ttatatcccc gctcgtcgac aaaggtcccg gcgcaaagcg
     2041 taaatgctat cgccaccagt gtcgcatcct tggacagggc atcgccgccg gccttcatgt
     2101 tatgcgtgtg taacagaccc acggcctcca ccaggaagaa aacgagatag gcgatgacga
     2161 atttgttcgt gaacgccgcc ttcacggccg gcaacggttt attctcccac aatttgtatg
     2221 cggccagcag gatgatacag aggctgttta tccggaacca ggagaaaacg gtggtcgcca
     2281 gcaccgcgaa ggtcaggtag agataaaggt gttttcggct catcaggatg caggccgggg
     2341 tttgtcagcg g
//
LOCUS       DATGFG010000248         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_721639, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000248 DATGFG010000000
VERSION     DATGFG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721639"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VKQ52_04615"
     CDS             <1..763
                     /locus_tag="VKQ52_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HLZ86492.1"
                     /translation="ALLIFVILHLLVYPASNGYNSYMDRDTTPIGGLERPPEPTRQLF
                     WVTLVMDLAALALGFFIGLYFVAGLAVYILASRAYSYRGIRLKKYPFIGWLTVISCQG
                     ALVFFLVYQGSHKAGVWAGTGSVAQAMLASSLLLGGFYPLTQIYQHEADRKDGVRTLS
                     LLLGLRGSFIFTGLVYGVAFLVLAHYLVFTYLEFKEFYVLATCMLPVIVYFLIWAVRV
                     WRDPAAASFANTMRMNLLASICTNAGFIALLVIGS"
     gene            824..1945
                     /locus_tag="VKQ52_04620"
     CDS             824..1945
                     /locus_tag="VKQ52_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HLZ86493.1"
                     /translation="MSKIISIGTAVPVHRHRQEDILPFMQRIYGLSSKEGPDFARSGE
                     AGRKLKFLYHQSGIETRYSVIPDYSLPVRYWQFYPPTENLEPFPSLELRMRWYREHAA
                     PLSLSAIRDCLQGVPDAGGITHLITVSCTGMSAPGLDLELVELLKLPPTVFRTSINFM
                     GCYAAVHALKIAHALAESEPGARILIVCTELCTIHFQQEPTPDNMLSSLLFSDGSAAA
                     LVVSDDHPGKGLRLRHFYSEIVPEGQKDMAWDLSSTGFRMTLSSYVTDSIRADFAGLV
                     RNALAKGGIDASRIGHWCIHPGGKKILDAIYTNLSFTNGHLDHSYAVLKQFGNMSSPT
                     ILFVLKRILEDLTHTQPNCIFGAAFGPGLTMETFIAETN"
     gene            1955..2680
                     /locus_tag="VKQ52_04625"
     CDS             1955..2680
                     /locus_tag="VKQ52_04625"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLZ86494.1"
                     /translation="MPGKPDFSRRSPEKELLDGEGLPFPDIERNMLELDVINTWLGGH
                     AISISGLASFVHNRKRIHICEIGCGGGDNLRVLSRWCRKRGIDVQVTGIDNNAHCLEV
                     ARQRWTGDNAGWIHADYRDVSFGNERPDILFSSLFCHHFTDEELVLQLRWMEEQAGAG
                     WFINDLQRHPLAYHSIRWLTAWFSGSYLVKNDAPLSVLRGFSRREWEGLLRRAEVGAY
                     SLRWKWAFRWLVVGGNGGGKRYV"
     gene            2673..>3532
                     /locus_tag="VKQ52_04630"
     CDS             2673..>3532
                     /locus_tag="VKQ52_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HLZ86495.1"
                     /translation="MYDVIIAGGGLAGLGLSIQLVRAGYRVALFEKETYPFHKVCGEY
                     VSLESWNFLEELGLPLSDWNLPIIRTLLMTAPSGLSLQQELPLGGFGISRYRLDAALA
                     DIARSEGVELHESTRVTDISFDRDVFTVRTSAGDFSARVACGTYGKRSNLDVRWKRAF
                     VRKRSTRLNNYIGVKYHVRAGLPADQIALHHFSQGYCGISKIEDDRYCLCYMTTAANL
                     QASGNSIAEMEATVLSRNPYLKELFASTVSLYHQPVTIAQISFEKKTQVEDHVLLIGD
                     AAGMAPPLSG"
BASE COUNT          697 a          952 c         1057 g          826 t
ORIGIN      
        1 cgcgctgctg atcttcgtga tcctgcacct gcttgtctat cccgccagta acgggtataa
       61 cagctatatg gatcgggaca caacgcccat cggcggcctc gaacggccgc ccgagcccac
      121 tcgccagctt ttttgggtga cgctggttat ggacctggca gcgctcgcgc tggggttttt
      181 tatcgggctc tacttcgttg cggggctggc ggtatatatt ctcgcgtcga gagcttatag
      241 ctaccgcggc atccgcctga aaaaatatcc gttcatcggc tggctgacgg tgatcagttg
      301 tcagggtgcg ctggtctttt tcctggtata ccagggcagc cataaggccg gggtatgggc
      361 cggcacgggg tcggtggcgc aggcgatgct tgcctcctcc cttttgcttg gcggcttcta
      421 tcccctgacg cagatctacc agcatgaggc cgaccggaag gacggggtga ggaccctcag
      481 cctgttgctg ggccttcggg gcagctttat tttcacgggg ctcgtctatg gggttgcttt
      541 tcttgttctc gcccattatc ttgtctttac ttacctggaa ttcaaagaat tttatgtgct
      601 ggcaacctgt atgcttccgg ttatcgttta ttttctgata tgggctgtca gggtctggcg
      661 ggatccggcg gcggcaagtt ttgccaacac catgcgaatg aacctgctgg cttcgatttg
      721 cacaaatgcc gggtttatcg cgttgctggt aattgggtcc tgacggccgg atggtcccat
      781 ctttgcaggt ttattctacg tatactatac taagcacaac ctattgagta agatcatctc
      841 catcggaaca gcagtgcctg tccaccggca tcgacaggaa gacatcctgc cctttatgca
      901 aaggatctat ggcctgagca gtaaagaagg gccggatttt gcccggagcg gcgaagccgg
      961 ccggaaattg aaattcctct accatcaaag cggcatcgaa acccggtatt cggtgatccc
     1021 cgattattcc ctgccggtgc ggtactggca gttctatccc ccgacggaga acctggagcc
     1081 cttcccttcc ctcgaactgc ggatgcgatg gtaccgcgag catgcggccc ctttatcgct
     1141 gtcggctatc cgcgattgcc tgcagggggt cccggatgcg ggcgggatca cccacctgat
     1201 aacggtgagc tgtacgggca tgagcgcccc cggcctcgac ctcgaactgg tggagctcct
     1261 gaagctgccg cccaccgtct tccgcacgtc gatcaatttt atgggttgct acgccgcggt
     1321 gcatgccctg aagatcgcac atgcattggc ggagagcgaa ccgggagcgc gtatcctcat
     1381 cgtttgcacc gaactatgca cgatccactt tcagcaggag cccacgccgg ataatatgtt
     1441 gtcgagcctt ttattttcgg atgggtccgc ggccgccctg gtcgtttcgg atgatcatcc
     1501 gggaaagggg ctgcggctac ggcatttcta ttcggagatc gtccccgaag gacagaaaga
     1561 tatggcctgg gacctgtctt cgaccggctt ccggatgacg ctcagcagct acgtcaccga
     1621 tagcatccgg gcggatttcg cgggcctggt ccggaacgcc ctcgcgaaag gcgggatcga
     1681 cgcgtcacgg atcggtcact ggtgtatcca tccgggcggc aagaagatcc tggatgccat
     1741 ctataccaat ctttccttta ccaacggcca tttggaccat agttatgcag tgctgaagca
     1801 gtttgggaac atgtcttcgc caaccatcct ctttgtattg aaacgcatcc tggaagacct
     1861 gacccatacc caaccaaatt gtatattcgg ggcggcgttt ggtccgggtc tcaccatgga
     1921 gacatttatc gcagaaacga attaacgtca tcgtatgcct ggcaaacctg atttttcaag
     1981 gagatcccct gaaaaggagt tgctggatgg ggaaggactt ccttttccgg atattgaaag
     2041 aaatatgctg gaactggatg tcatcaatac ctggctgggc gggcatgcga tcagcatcag
     2101 cggcctggcc tcgtttgtcc acaaccggaa gcggatacat atctgcgaga tcggttgcgg
     2161 cggcggggat aatctccggg tgctgagccg gtggtgccgg aagcgcggta tcgacgtaca
     2221 ggtcacgggc atcgacaaca acgcccattg cctggaagtg gcgcggcagc gatggacggg
     2281 cgacaatgcc ggctggatcc atgcggatta ccgggatgtg tcctttggga acgaaagacc
     2341 ggatatcctt ttttcatcgc tgttctgtca tcatttcacc gatgaggagc tggtcctgca
     2401 gctccggtgg atggaggaac aggccggggc cggctggttt atcaacgacc tgcagcggca
     2461 tccgctggcc tatcattcga tccggtggtt gaccgcctgg ttttcggggt cctatctggt
     2521 aaagaacgac gcccccttgt cggtattgcg gggctttagc cggagagaat gggagggctt
     2581 gttacgacgg gcggaggtcg gcgcatattc tttacggtgg aaatgggcgt tccgctggct
     2641 ggttgtaggt gggaatgggg gagggaagcg atatgtatga tgtcattata gcaggaggcg
     2701 gcctcgccgg cctcgggctt tcgatccagc tggtccgggc cggttaccgc gtggcccttt
     2761 ttgaaaagga gacctacccc tttcacaagg tttgcgggga atatgtcagc ctggaaagct
     2821 ggaatttcct ggaagagctg gggctgcccc tgagtgactg gaacctgccg atcatccgga
     2881 ccttgctgat gaccgccccc agtggcctgt cgttgcaaca ggagctgccg ctcggcggtt
     2941 ttgggatcag ccgctaccgg ctcgacgcgg cgctggcgga tatcgcccgg tcggaaggag
     3001 tggaattgca tgaatcgaca agggtgaccg atatctcctt cgatcgcgat gtcttcaccg
     3061 tacggacaag cgccggagat ttttcggcca gggtagcgtg tggaacatac gggaagcgaa
     3121 gcaatctcga cgtccgctgg aaacgcgcct ttgtccggaa gcgatcgacc aggctcaaca
     3181 attatatagg cgttaaatac catgtcaggg ccggcctgcc cgccgaccag atcgcactgc
     3241 accatttttc ccagggttat tgcggcatct cgaagatcga agacgatcgc tattgtcttt
     3301 gctatatgac aacggccgcc aatctgcagg ccagcggcaa ttctatcgcg gagatggaag
     3361 caaccgtgtt aagcaggaat ccgtatctca aggagttgtt cgcttcgacg gtcagtctct
     3421 atcatcagcc ggtgacgatc gcacagatca gctttgagaa aaagacccag gtggaagatc
     3481 atgtcctgct gatcggggat gcggcaggga tggcgccgcc tctatccggc aa
//
LOCUS       DATGFG010000249         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_153105, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000249 DATGFG010000000
VERSION     DATGFG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153105"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="ricT"
                     /locus_tag="VKQ52_04635"
     CDS             complement(<1..971)
                     /gene="ricT"
                     /locus_tag="VKQ52_04635"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HLZ86496.1"
                     /translation="MGCSNCGTSNGGKPSGCKSNGGCSTGGCNRLNVYDWLSNLPLSD
                     ADGACRVLEVSFNQGSRKEFYRNATTQVFEKGDCVSVEGVSGFDVGEVTMTGELVRLQ
                     LKKKGIEEKNPDIKKVLRRASDRDIEVMQQNKAREKEALIRSRAIARKLKLDMKMSEV
                     EIQADGRKATFFYIADDRVDFRELIKLYAGEFRLKVEMRQIGARQEAAKVGGVGSCGR
                     ELCCSTWLSDFKSVNTTAARYQNLSINQSKLSGQCGRLKCCLNYELDTYLDALQNFPD
                     NAESLQVAKGTASLIKKDIFKNLMWYVLPDSNKQYPLTIERVRKIRQ"
     gene            complement(1220..2356)
                     /locus_tag="VKQ52_04640"
     CDS             complement(1220..2356)
                     /locus_tag="VKQ52_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86497.1"
                     /translation="MAFTSVIGQGQVKQHLVELIQGNRLSHALLFLGQEGSGALPLAL
                     AFAQYVVCEHPRDGDACGVCPPCVKASQLIHPDIHYSYPVIPKKAGDKPVSTDYGAEW
                     REFIAQYPYGNAYDWLQFIGAENKQGNITAQECNDIIHKLSLKSFESGYKILLMWMPE
                     YLGNEGNKLLKLIEEPPANTLFLLVAENESLILPTILSRTQLVKVPLPETAEIERALV
                     ERAGLGVEQARQTAVLCEGNYHEALQLIRHADEDWQGVLREWLNAILKNGPIAQVKWI
                     DEISKSGRERQKQFLRYFNHLLEQAIRLRYIDGGGVPIPEGERDIALRLNKIADVGQQ
                     RAIIEELDRAAYYIERNAHAKMLFHALTIKVYHILADRKVVVVN"
     gene            complement(2432..>2800)
                     /locus_tag="VKQ52_04645"
     CDS             complement(2432..>2800)
                     /locus_tag="VKQ52_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HLZ86498.1"
                     /translation="GYTPQYADLEKLYEQYKDKMVVIGFPANNFGQQEPGTAQEITEF
                     CTKNYGVTFPMAEKGDVIGDNIQPLFKYLTDEAHKLGVADPVVKWNFTKFLVDENGKL
                     VAVFPSKVKPMDPEITKYLN"
BASE COUNT          628 a          912 c          611 g          649 t
ORIGIN      
        1 atttgccgga tcttccgcac gcgctcgatc gtcagcgggt actgcttgtt gctgtccggc
       61 aggacatacc acatgagatt cttgaagata tctttcttta taaggcttgc cgtacccttc
      121 gccacctgca ggctctccgc attgtccggg aaattttgca gggcatcgag ataggtatcc
      181 aactcgtagt taagacaaca tttaaggcgg ccgcactgcc cgctcagttt actctggtta
      241 atggacaggt tctgataccg cgccgcggtc gtattcaccg atttgaaatc gctgagccag
      301 gtgctgcaac acaattcccg cccacagctg ccgacaccac ccaccttcgc cgcttcctgc
      361 cgcgcaccga tctgccgcat ctccaccttc aggcggaatt cccccgcata gagcttgatc
      421 agctcccgga aatccacgcg gtcatcggcg atataaaaga acgtggcctt tcttccgtca
      481 gcctggatct ctacctcgct catcttcata tcgagcttga gcttcctggc gatcgcccgg
      541 ctccggatca gggcttcctt ttcgcgggcc ttgttctgtt gcatcacctc gatatcccgg
      601 tcactggccc gccgcaacac ctttttaata tccgggttct tttcctctat ccccttcttt
      661 ttcaattgta aacgaaccaa ttcacccgtc atcgtcacct ctcccacatc aaacccgctg
      721 accccttcca cactgacgca atcccccttc tcgaatacct gtgtcgtggc gttgcgataa
      781 aactccttcc ggctgccctg gttgaagcta acctcgagga cccggcacgc gccgtcagca
      841 tcactaagtg gaagattgga aagccagtca tatacattca accggttgca tccgcccgta
      901 ctgcacccgc cattgctctt gcatcctgaa ggcttgccgc cattggatgt gccgcaatta
      961 ctacatccca tatcctaaaa tgaattaaga agtaaaaaaa taaatatatg tgccgagaaa
     1021 agaaaacccg tacaggcagg aagacgctac ttgtagacgt acactaaaga aaatatgctg
     1081 aatacaaaat aacagttttc caatggtccc ggcgcaaatc acatatgcac actcaccgcc
     1141 ggcccatccc cggattgccc ttctttcacc tggcctcgaa cggccatggg gtaaatatac
     1201 accgaatcag gcattccgcc taattcacca ccaccacctt cctgtccgcc aaaatatgat
     1261 agaccttgat cgtcagggca tgaaaaagca ttttcgcatg ggcatttctt tcaatataat
     1321 acgccgcccg gtccagctcc tctataatgg cccgctgttg ccccacatcg gctatcttat
     1381 tcaaccgcaa agctatatcc cgctccccct ccgggatcgg cacccccccg ccgtcgatat
     1441 accgcaaccg gatggcctgc tccaaaagat ggttgaaata ccgcaggaac tgcttctgcc
     1501 gctcccgccc cgacttgctg atctcatcga tccacttcac ctgggcgatc ggcccattct
     1561 tcaggatggc gttcagccac tccctcaaga ccccctgcca gtcctcgtcc gcatgccgga
     1621 tcagctgcaa cgcctcatgg taattgccct cgcaaagcac cgccgtctgc cgggcctgct
     1681 ccaccccaag accggcccgc tccaccagcg cgcgctcgat ctccgccgtc tccggcaagg
     1741 gcaccttcac cagctgcgtc cgggacagta tcgtaggcag gatcagcgac tcgttctccg
     1801 ccaccagcag gaacagcgta ttcgccggcg gctcctcgat cagcttcaac agcttgttcc
     1861 cctcattgcc aagatactcc ggcatccaca tgagcaggat cttatagccg ctttcaaaac
     1921 tcttcaggct cagcttatgg atgatatcat tacattcctg cgcggtgata ttcccctgct
     1981 tgttctccgc cccgatgaac tgaagccagt cataggcatt gccataggga tactgcgcga
     2041 tgaattcccg ccattccgcc ccataatccg tactcaccgg cttgtcaccc gcttttttgg
     2101 ggatcaccgg gtaggaataa tgaatatccg ggtggatcag ctggctcgcc ttgacacagg
     2161 gcgggcaaac cccacaggca tccccgtccc tgggatgctc acagacgaca tactgcgcaa
     2221 aggcaagcgc cagcggcagc gcaccgcttc cctcctgccc gaggaataaa agggcatgac
     2281 tcaggcgatt gccctggatc aactccacca ggtgttgctt cacctgcccc tgcccgataa
     2341 cggatgtaaa tgccatgggc gcaaaaataa aaaaaaggcc ggcgcgacca tgataaatcg
     2401 caccagcccc tctattaaag attcgaatac gctaattcag atacttggtg atctccgggt
     2461 ccatcggctt caccttgctg ggaaacaccg ccactaactt cccattctcg tcgaccagga
     2521 atttggtaaa attccatttg accacgggat cagccacccc cagcttgtgc gcctcatcgg
     2581 tcagatactt gaacagcggc tggatattat ccccgatcac atcccccttt tccgccatgg
     2641 ggaaggtgac gccatagttc ttggtgcaga attcggtgat ctcctgggcc gtacccggct
     2701 cctgctgacc aaaattgttg gccgggaaac cgataactac cattttatcc ttgtattgct
     2761 catacaactt ttccaggtcg gcatattgag gggtataacc
//
LOCUS       DATGFG010000250         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_832316, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000250 DATGFG010000000
VERSION     DATGFG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832316"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VKQ52_04650"
     CDS             complement(<1..1297)
                     /locus_tag="VKQ52_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HLZ86499.1"
                     /translation="MSYQPIENYGIVGNLHTIALVGLNGSIDFMCFPQFDSPSVFARL
                     LDDKKGGFFCIAPEFGEMKTKQLYLPDTNVLLTRFLSPDGVGEITDFMPLGLKEERKL
                     IRRITTIRGEVKYRLQCSPRFNYGKDQYQARQTGPNEILFHADGPNGLSVRLKAGFPL
                     QLSDNDAVAEFTLPAGDTADIILENADNENIGSETLKERVTQHLISTINYWKDWVSRS
                     TYRGRWMETVNRSALVLKLLSSAQYGSIIAAPTFGLPETIGGKRNWDYRYTWIRDASF
                     TVYSFIRLGYTKEAGAFMGWIEKLCYDIKEGERLGIMYSIDGKRQLQEHTLDHFEGYK
                     GSTPVRVGNDAYAQLQLDIYGELMDSVYLYNKYGEPISYDFWKDLEKQIEWLSRNWDQ
                     PDDSIWEVRGGRKKFLFSRLACWVALDRAMKIAQARSFPR"
     gene            complement(1391..2467)
                     /locus_tag="VKQ52_04655"
     CDS             complement(1391..2467)
                     /locus_tag="VKQ52_04655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLZ86500.1"
                     /translation="MPLREIIINTIRIHGPLSFHDFMEMALYYPGQGYYAAPRDKIGE
                     AGDFYTSPCLTSLFGETIAGQLEEMWVLLGCRPFTIVECGAGQGLLCRDILHRLRANS
                     QLFGHLQYIIIEKSEWMRQKEQKLLAAEGFLPKLHWADSLRSLPHIHGCILSNELVDN
                     FAVHQVVMQDELMEVFVGYDEGFVEVLRPASAPLKEYLQELRITLPKGYRAEINLEAT
                     EWIHDISSALSSGFVLTIDYGHSAADLYSRRTGTLACYHQHQVHHCPYDFIGEQDITT
                     HVNFSALDHWGRRYGLEYCGYTSQARFLQGLGLTARLHHLGASGQQLRTLLLDMGNKL
                     KVLIQRKGLPRTLLTGLQFAQTLA"
     gene            2505..>2947
                     /locus_tag="VKQ52_04660"
     CDS             2505..>2947
                     /locus_tag="VKQ52_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86501.1"
                     /translation="MPTTTLEKEPAAAAAKDIEQLCIDTVRVLSADAVQKANSGHPGT
                     PMATAPMGHLLWGKHLRYNPRDPAWPNRDRFVLSAGHACMLQYSFLYLTGYDLTLDDI
                     KNFRQLHSKTPGHPEYGLTPGIEVTTGPLGQGFANGIGLAIAQKH"
BASE COUNT          614 a          722 c          935 g          676 t
ORIGIN      
        1 tgcgggggaa ggagcgggcc tgggcgatct tcatagccct gtccagggcg acccagcagg
       61 cgaggcggga gaagagaaac ttctttctgc caccccggac ttcccagatg ctgtcgtcag
      121 gctgatccca gttgcggctc agccattcga tctgtttttc caggtccttc cagaaatcat
      181 aggagatggg ctcaccatac ttgttataca aatatacgga atccatcaac tctccataaa
      241 tgtccagttg caactgtgca taggcgtcgt tgcctacgcg aacgggtgtg ctgcctttat
      301 agccctcgaa atgatccagg gtatgttcct gcagttgccg tttgccgtct atggaataca
      361 tgatccccag tcgctcgccc tctttgatat cgtagcagag tttttcgatc cagcccataa
      421 acgcgccggc ttcctttgta tagcccaggc gaataaagga gtagaccgta aaggaggcgt
      481 cgcggatcca ggtatagcga tagtcccagt tccgtttgcc gccgatggtc tcggggaggc
      541 caaaagtggg tgcggcgatg atggacccat actgcgccga agacagcagt ttgaggacga
      601 gggccgaccg gttgaccgtt tccatccacc ggcccctgta ggtagaccgg gagacccaat
      661 ctttccagta gttgatcgtc gagatcaggt gttgtgtaac tctttccttc agggtttctg
      721 atccgatgtt ctcgttgtcg gcgttctcga ggatgatgtc ggcggtgtca cccgcgggga
      781 gggtaaattc ggccacggcg tcattgtcgc tgagttgtag ggggaagccg gcctttagcc
      841 ggacactcag cccgttgggg ccatcggcat gaaagaggat ctcgtttggg ccggtctgtc
      901 ttgcctggta ttggtccttt ccgtaattga agcggggtga acactgcagc cggtatttta
      961 cctcaccgcg gatggtggtg atgcggcgga tgagttttct ttcttctttt aaccccaagg
     1021 gcatgaagtc ggtgatctcc cctaccccat cgggagatag aaagcgggtc agcaggacat
     1081 tggtatcggg gaggtagagt tgttttgtct tcatttcgcc gaattccggg gcgatgcaaa
     1141 agaagccgcc ttttttatcg tcgaggagcc gggcaaagac gctgggggaa tcgaattggg
     1201 ggaagcacat gaagtcgatg gaaccgttca ggccaacgag ggcaatagtg tggaggttac
     1261 cgacgatgcc ataattttcg atgggttggt agctcactgt ggtgatttat ggagcgcttg
     1321 caaaaattgg gcccgggtta actccgtggt tttccggggt taatggtgat agcctggtag
     1381 cgaccgtgat ctacgccagg gtttgtgcga attgcaagcc ggtgaggagg gtgcggggga
     1441 ggcctttgcg ttggatcagg actttgagtt tgttgcccat atcgaggagg agggtgcgta
     1501 gttgttggcc cgaggcgccg aggtggtgga ggcgggcggt gaggccgagg ccctggagga
     1561 agcgggcctg gctggtgtag ccgcagtatt cgaggccgta gcggcgaccc cagtggtcga
     1621 gggcggaaaa gttgacatgg gtggtgatgt cctgttcgcc gatgaagtcg tatgggcagt
     1681 gatgcacctg gtgttggtga taacaggcca gggtgccggt gcggcggcta tataggtcgg
     1741 cggcggaatg accgtagtcg atggtcagta cgaagccgga tgacaatgcg gacgagatgt
     1801 catggatcca ttccgtcgcc tcgaggttga tctcggcccg gtagcctttt ggcaatgtga
     1861 tccggagttc ctgaaggtat tccttcagcg gggcggaggc ggggcgaagg acttcgacga
     1921 agccctcgtc gtaaccgaca aatacttcca tgagttcgtc ctgcatgacc acctggtgca
     1981 cggcgaagtt gtccaccagt tcgttggaga ggatgcagcc atggatatgc gggagggagc
     2041 gaagggaatc tgcccagtgc aattttggca ggaagccttc ggcggcgagg agtttctgtt
     2101 ctttttggcg catccactcg cttttttcga tgataatgta ttgaagatgc ccgaacagtt
     2161 ggctattggc gcgcaggcga tggaggatat cgcggcagag cagaccctgt ccggccccgc
     2221 attcgacgat cgtaaacggc cggcagccga ggagcaccca catctcttcc agttgaccgg
     2281 cgatcgtctc accgaaaagg ctggtcagac aggggctggt gtagaagtca cctgcttcgc
     2341 cgatcttgtc gcggggtgcg gcgtaatacc cctgtccggg atagtaaagc gccatctcca
     2401 tgaaatcgtg aaaggaaagc ggaccgtgga tgcggatggt atttatgatg atctcccgaa
     2461 gcggcataga aattgagcct tttgggtgaa gcaaatcaat tgttatgccc actactacat
     2521 tagaaaaaga gccggcagca gcagctgcaa aagacatcga acagttgtgt atcgacaccg
     2581 tgagggtctt atccgccgat gcggtgcaaa aggccaactc gggccacccg gggacaccga
     2641 tggcgacggc gccaatgggg catcttctat ggggaaagca tttgcggtac aatcccaggg
     2701 atccggcgtg gcccaacagg gacaggtttg tattatccgc ggggcatgct tgtatgctcc
     2761 aatacagctt tctttatctc accggttatg atctcaccct tgacgacata aaaaatttcc
     2821 ggcaattaca cagcaagacc cccggtcacc cggagtatgg gttgacgccc ggcatcgagg
     2881 tcacgacggg gccacttggc cagggcttcg ccaacgggat cggtctcgcc atcgcgcaga
     2941 agcattt
//
LOCUS       DATGFG010000251         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_951380, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000251 DATGFG010000000
VERSION     DATGFG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951380"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..209
                     /locus_tag="VKQ52_04665"
     CDS             57..209
                     /locus_tag="VKQ52_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86502.1"
                     /translation="MGTRLALRRSALSRASERVFTEAGALPGLNAGAFVGEVDLYGAD
                     SGFALN"
     gene            311..>2043
                     /locus_tag="VKQ52_04670"
     CDS             311..>2043
                     /locus_tag="VKQ52_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HLZ86503.1"
                     /translation="MKRIILLLLTLNTVIRLFAQPASGNPAPFVIPALQEWKGGSGSF
                     VITAATSIIVEAASADSLLPSAQILQEDLRQLTGGRPLPVRTGSPAPGAIYLGLHAPD
                     QALGPEGYLLAADQGGLQISALRTRGLFWGTRTLLQVLEQDSLHTQFPCGFARDYPRY
                     GIRGFVLDDGRKFFSLDFLRQYVRFMAYYKMNDFHIHLNDNGFYKSFGGNWDSTYSAF
                     RLQSDTYPNLTAKDGAYTKQEFRDLQALARSYGVNIVPEIDVPAHSLAFTKLRPEIGS
                     RKYGMDHLDLDNPVTYQVIDSIFMEYLQGPNPVFTGPEVHIGTDEYAKAAAEQFRAFT
                     DHYIRLVQRYGKKVRLWGALTHAKGNTPVTVKDVTMNVWYNGYADPREMKKLGYDMIS
                     TPDGWLYIVPAAGYYYDYLELPELYDKWEPVQVGDVRFDAGDPQIKGGSFAVWNDKVG
                     NGITEKDVHDRVFPAMQVLSQKMWRGTDSSRPYQAFALAATRLGEGPGANMRGKLSAQ
                     SGVVMQYEFTQSSGTRLTGIGNNHPAGNGKTDLSGNGRHATKLVKAKIGKDGLCLKGG
                     KSYLRTPVKEIG"
BASE COUNT          489 a          600 c          522 g          432 t
ORIGIN      
        1 aatggaaaaa gccgttctgg aagtagcagt tcattaagcg gaataaatta ataagaatgg
       61 gaaccaggct tgccctgcgg cgatctgcgc tgtcccgtgc cagtgaaaga gtatttacgg
      121 aagctggcgc tctgcccggc cttaatgcgg gcgccttcgt cggcgaagtt gacctgtatg
      181 gcgcggatag tggattcgcg ctgaattgac atttcgcctt acgcaaacga ttgctcgaaa
      241 taggtcgcat gtaaatattt tatctcaatt ttacagcttc tgaaaaaata taacaaagta
      301 tttacatagc atgaagcgta ttatcctcct cctcctcaca ctgaacaccg tcataaggct
      361 ttttgcgcaa cctgcgtcgg gtaatcccgc ccccttcgtc attcccgcgt tgcaggaatg
      421 gaaggggggc tccggcagct ttgtcatcac agccgctacc tccatcatcg tcgaagcagc
      481 atccgcggac agcctgcttc cttccgcaca gatccttcaa gaggatctgc gacaactgac
      541 cggaggacgg ccattacccg tccggacagg atctcccgcg ccaggcgcca tctaccttgg
      601 cctccacgcc cctgatcagg cattggggcc tgaaggctac ttgttagcgg cagaccaagg
      661 cggtctgcaa atatccgccc tccggacccg gggactcttc tggggcaccc gcaccctcct
      721 tcaggtactc gagcaggata gccttcatac gcaattcccc tgcggctttg caagagatta
      781 tccccgctac ggcattcgcg gcttcgtatt ggatgatgga cgcaaattct tcagcctgga
      841 cttcctccgc cagtacgtca ggttcatggc ctattacaaa atgaatgatt tccacatcca
      901 tctgaacgat aacgggttct ataaatcttt cggcggcaac tgggatagca cctattccgc
      961 tttccggctg caaagcgaca cttatcccaa cctcaccgcc aaagacggcg cttatacgaa
     1021 acaggaattc agggacctcc aggcccttgc ccgctcttat ggcgtcaata tcgtccccga
     1081 gatcgacgtc cccgcccact ccctcgcctt caccaaattg cgccctgaaa tcggcagcag
     1141 gaaatacggc atggaccacc tcgacctgga taaccccgtc acctaccagg tcatcgacag
     1201 catattcatg gaatacctcc agggccccaa ccctgtattc accgggcctg aagtccatat
     1261 cggcaccgac gaatatgcca aggccgcggc cgaacagttc cgggccttca ccgatcacta
     1321 tatccggctg gtacaacgtt atggcaagaa agtgaggttg tggggcgccc tcacccatgc
     1381 aaagggaaat acgccggtca cggtgaaaga cgtgaccatg aatgtctggt acaatggcta
     1441 tgccgatccc agggaaatga agaaactggg ttacgatatg atttccactc cggacggatg
     1501 gctctatatc gtgcccgcag ccggctacta ctatgattat ctcgaactcc ccgaactcta
     1561 tgacaaatgg gaacccgttc aggtaggcga tgtacgcttc gatgcaggcg atccgcagat
     1621 caagggcggg tccttcgccg tctggaatga taaggtcggc aatggcatca ccgagaaaga
     1681 tgttcatgac agggtattcc ccgccatgca ggtactgtcc cagaaaatgt ggcgcgggac
     1741 cgacagctcc cggccctacc aggctttcgc cctcgcggcc acccggctgg gggaagggcc
     1801 cggcgcgaat atgcgcggaa agctctccgc gcaatcggga gtggtcatgc aatatgagtt
     1861 tacgcaaagc tccggaaccc gccttacggg cattggcaac aaccatcctg ccggaaatgg
     1921 aaaaacggat ctttcgggca atggccgcca tgcgacaaag ctcgtgaaag caaagatcgg
     1981 caaggatggc ctctgtttaa agggcggtaa aagttatctc cggacccccg taaaggaaat
     2041 tgg
//
LOCUS       DATGFG010000252         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_699993, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000252 DATGFG010000000
VERSION     DATGFG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699993"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VKQ52_04675"
     CDS             <1..523
                     /locus_tag="VKQ52_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86504.1"
                     /translation="EILDEFRKKLLLTIYFRGKELYAGLTYTDTVQGSSVSYQLGWNT
                     IRDDQLKFREAKSQQLRIIAIHKLKKGGKIQTEVGDKNAAVRTVFFPNVTDTTTLKQL
                     AEKKLSELKYDGYEGKITAFLQPFCDIAYKGIVTDPRYPQRSGHYFIDTVEVEYSTKG
                     GRRKVEIGIKLTS"
     gene            523..1023
                     /locus_tag="VKQ52_04680"
     CDS             523..1023
                     /locus_tag="VKQ52_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86505.1"
                     /translation="MLWEERIRQGMIDMASKHGPAQIVTGTVLDVDETQDTCTVTTVD
                     GLEIPEVQLRATINPDERKAWVLLPAVNSEVLIGNIENGTQWCVLMVSEITGIRSEVN
                     NTVFEVDGEKFQLSNDQENLSTLIRDLITTIREMVFTTNVGPTIQLVNDQAFQSLQTR
                     FNNLLK"
     gene            1027..1248
                     /locus_tag="VKQ52_04685"
     CDS             1027..1248
                     /locus_tag="VKQ52_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86506.1"
                     /translation="MPTNQTRLTDAIQAALEAQVGNQTDAGAARQQLASSIASAVLVE
                     IKAAQILYEGGLIDGTGKPVSGVFTNSIN"
     gene            1248..1538
                     /locus_tag="VKQ52_04690"
     CDS             1248..1538
                     /locus_tag="VKQ52_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86507.1"
                     /translation="MPQDIVFASDGDIDFSANDLQYGESTAQHQRDILLARKGDYKMS
                     PTVGVGIMDYLLDHTPDEMQREIRQQLYQDGMDVNKITYSNGLLTIDAPYAS"
     gene            1561..1878
                     /locus_tag="VKQ52_04695"
     CDS             1561..1878
                     /locus_tag="VKQ52_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86508.1"
                     /translation="MDMAVQGYGDVMQVFTLIGDNVGLYTMRDNGGQFLVDEVGVIDL
                     GYPVKKGLTYTVQDGSDLQNKQVLQALPAEFLTTCEDFYRSPDTSLGDFNADFNNDFN
                     ISR"
     gene            1875..3083
                     /locus_tag="VKQ52_04700"
     CDS             1875..3083
                     /locus_tag="VKQ52_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86509.1"
                     /translation="MKTFFFFTTALLIAVMASAQFQSTDELRQYNNNYIRPNGHQSIT
                     AQQLNNLHAGMLDFIDTALLNRAKSIRISKDTIYLQLGTGSEIPIKLPPQVNADWNAV
                     SGPAQIVNKPVIPPFQVNADWNATGGVAQILNKPTIPAAQVNADWNAISGPAQILNKP
                     GTPMLSDVLTAGNLAIPGTTTQAPIVMQLGDLLTTPQAGAVEYDGLALYITDGQSVRH
                     NLTQTDRVFKVYSWDYNNDTLFFSLTGWDVQYVGDPTYAPTVASTIDVEITVTGGTPA
                     GSVITMQFEGQTVYTLTVTETAGTDYYVIGTVKVNQDIKYPEVFYIKGAVETINGAGS
                     IQSANTAYRYTSDGFTNPVLTFGSSASTLIRIGCTYTVDKKQQGRWIYSPLPGNPFTP
                     PPPPSGGGNF"
     gene            3094..3489
                     /locus_tag="VKQ52_04705"
     CDS             3094..3489
                     /locus_tag="VKQ52_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86510.1"
                     /translation="MALTTTKISNHKVFPTVERTVLIDSFQVVDSRKEIFVYYTISYQ
                     QNGTDVTAQFSKKQLPITANNTKSIMVRDDNMQPVPNPKWDGKDPATQYEMQPAYDNL
                     IQYLTKAVPVATILNAYIQVNDADGFFDK"
BASE COUNT          965 a          871 c          938 g          750 t
ORIGIN      
        1 ggagatactc gatgagttta ggaaaaagct gctcctgacc atttatttcc gggggaagga
       61 actgtatgcg ggactgactt acaccgatac cgtgcagggt agttccgtat cctatcaatt
      121 aggctggaac acgatccggg acgatcagtt gaaattccgg gaagccaaat cacagcaatt
      181 gcggattata gcgatccaca aattgaaaaa aggcggtaag atacagacgg aggtgggcga
      241 taagaatgca gccgttagaa cggttttctt tcccaacgtg accgatacga ccaccctgaa
      301 gcaactggcg gaaaagaaac tgagcgagtt gaaatatgat gggtacgagg ggaagataac
      361 ggcattcttg caaccctttt gcgacatcgc gtacaaaggg atcgttacag acccccggta
      421 tccgcaacgg agtggccatt atttcatcga taccgtcgaa gtggaatatt cgacaaaagg
      481 aggcaggcgc aaagtagaga tcggcattaa actgaccagc taatgttatg ggaagaacgg
      541 atacggcagg ggatgatcga tatggcgagc aaacatggcc cggcgcagat tgttaccggg
      601 acggtcctgg acgtggacga gacccaggat acctgcacag ttaccacggt ggacggcctg
      661 gaaataccgg aggtgcaact gagggctacc atcaacccgg atgagaggaa agcctgggtg
      721 ttgcttccgg cggtgaatag cgaagtgctg atcggcaata ttgagaatgg gacgcagtgg
      781 tgtgtgctca tggttagtga gatcacgggg atcaggagcg aggtgaataa tacggtcttt
      841 gaagtggacg gcgagaagtt ccaactcagc aacgaccaag agaacctgag caccctgatc
      901 agggacttga tcaccaccat ccgggagatg gtttttacga cgaacgtagg gccgaccatt
      961 cagttggtca atgaccaggc ttttcaaagc cttcaaaccc ggtttaataa ccttttaaag
     1021 tgagttatgc caaccaacca aacgaggtta acggacgcga tacaggcggc actggaggcg
     1081 caggtcggca accagaccga tgcaggagcc gcccgccagc agcttgcttc ctccattgct
     1141 tcggctgtcc tggtagaaat aaaggccgcg cagattctgt atgaaggcgg gctgatagac
     1201 gggaccggaa aaccggtatc gggagtgttc accaatagta ttaactaatg ccacaggata
     1261 ttgtatttgc cagcgatggg gacattgatt ttagcgcaaa tgatctccag tacggggaaa
     1321 gtaccgcgca acaccaacgg gatattttgc tggcccggaa gggcgattac aaaatgtcgc
     1381 ctaccgtggg ggtggggatc atggattacc tgctcgacca taccccggat gaaatgcagc
     1441 gcgagatccg ccagcagctt taccaggacg gcatggatgt gaacaagata acatatagca
     1501 atggattact cacgatagac gcgccttatg caagttagtg tggctgataa acaaagtcta
     1561 atggatatgg ccgtccaggg atacggggac gtgatgcagg tatttaccct gatcggtgat
     1621 aacgtgggcc tatataccat gcgagacaac ggcgggcagt tcctggtgga tgaggtgggt
     1681 gtaattgacc tgggctatcc ggtaaaaaag ggacttacct ataccgtcca ggacggaagc
     1741 gacctccaga ataagcaggt attgcaggca ttaccggctg agtttttgac gacctgcgag
     1801 gacttctacc ggtcaccgga tacgtctctc ggagacttta acgcggattt taataacgac
     1861 tttaatattt cccgatgaaa acttttttct tcttcaccac ggctttactg atagcggtca
     1921 tggcatcggc gcaatttcaa agcaccgatg agctacggca gtacaataac aactacattc
     1981 gaccaaatgg ccaccaaagc atcacagcgc agcagctcaa caacctgcat gcgggtatgt
     2041 tggattttat tgacacggcg ctgctgaacc gggccaaaag tatccgtatc agtaaggata
     2101 cgatctacct gcaattaggg accggctcgg aaataccgat aaagttgcct ccccaggtga
     2161 acgcggattg gaatgctgtt tccggccccg cgcagatagt gaataagccc gtgattcctc
     2221 ctttccaggt aaacgcggac tggaacgcga ccggcggagt agcgcaaata ctcaataagc
     2281 cgactattcc tgccgcccag gtgaatgcgg attggaatgc gatcagcggt ccggcccaaa
     2341 tactcaataa gccggggacg cctatgcttt ccgatgtgtt gacagcgggc aatctcgcca
     2401 taccgggaac aaccacccag gctcccatcg ttatgcagct aggggattta ctcaccaccc
     2461 cccaggcggg tgcggtggaa tatgatggcc tggccttgta tattacggac gggcagagtg
     2521 tacgacacaa cctcacccaa accgaccggg tatttaaagt ctattcctgg gactacaata
     2581 acgatactct ttttttctcg ctcaccgggt gggatgtgca gtacgtaggc gatccgacct
     2641 atgcccctac ggtggcctcg accatcgacg ttgaaataac cgtgacgggt ggcacccctg
     2701 ccggatcggt tattaccatg caatttgaag ggcaaaccgt ctacacgttg actgtaacgg
     2761 aaacagcggg gaccgattac tacgtgatcg ggaccgtcaa agtgaaccag gacattaaat
     2821 acccggaggt attctacatc aaaggcgcag tggaaacaat aaatggagcg ggcagcatcc
     2881 agagcgccaa tactgcctac cgctatacct ccgatggatt taccaacccg gtattaacgt
     2941 ttggcagctc tgcctctacc ctcatacgaa taggatgtac ctataccgtg gacaaaaagc
     3001 agcagggacg atggatttat tccccgctgc caggcaaccc atttactcca ccaccacccc
     3061 cctccggcgg cggcaatttt taaattttct aatatggcac taacaaccac caagatcagt
     3121 aaccacaagg ttttccctac cgtagaaagg accgtcttaa ttgattcctt ccaggtcgtg
     3181 gacagccgga aagaaatttt cgtgtactac acgatcagct accagcaaaa tgggacggat
     3241 gttaccgctc agttttcgaa gaagcagttg cccatcacgg ctaacaacac caaaagcatc
     3301 atggtccgcg acgataacat gcagcccgta cccaacccga aatgggacgg caaggacccg
     3361 gccacgcagt acgagatgca gcccgcctac gataacctga tacaatacct tacgaaggcc
     3421 gttccggtgg ctactatctt gaatgcctat atccaggtta acgatgcgga cggctttttt
     3481 gacaagtaaa gcagctttaa tgtttgttta aagagtgaat aatg
//
LOCUS       DATGFG010000253         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_607449, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000253 DATGFG010000000
VERSION     DATGFG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607449"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..995)
                     /locus_tag="VKQ52_04710"
     CDS             complement(315..995)
                     /locus_tag="VKQ52_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ86511.1"
                     /translation="MKVYFISGIGADYRLFTHLRLPEGFEAAYVHWIPPAPNEKLADY
                     AIRLTAQIDTTQPFALVGLSLGGIMSVEMAKHITPLCTILISSVPVSAHLPRFYRMAG
                     NLGLGKLIPASLLKAATTVKHSLMMRPAANRQLMMSIIRDGDDTFIRWALTAVLEWEN
                     TRIPQPLYHIHGTRDEVFPIHLTTPTHTIRKAGHMFLLYRADTVNRILREILLPFSTP
                     PIPNRTPA"
     gene            1036..1962
                     /locus_tag="VKQ52_04715"
     CDS             1036..1962
                     /locus_tag="VKQ52_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ86512.1"
                     /translation="MSRKEIIPETLYELYRWLGLPLDLLDPREEFTIHNVRDLLTELP
                     YTSPSYRPNFFNFLFIKDARGRYTIDEMAFDLEPGTIYFTNPGVYRTFGWREIGEAYL
                     ITFNETYLKEYVHPEIYAEFPFLLTETIRPLRLDPAAFTEFERLYTQIFEEQRGDSPY
                     KKKIIGNLFVILLLKIKARFWKDYNPIYEGNRGSQIVKSFRRDLEKHFRDLIHGKAEK
                     VFRASEYAALQSLHPSYLNNVIKSKTGKPITVWIAEKTVSGAKSLLQQPGMSIKEIAY
                     TLGFAESTHFSAFFRKHTGHSPADYRKQFVKA"
BASE COUNT          417 a          434 c          704 g          465 t
ORIGIN      
        1 gctggagatg gagccgccgg gttggattgg atggccgaga cgggggcctg ggcggggaag
       61 gtcggctggg gggcccgggc ggccggatag attggggcca ttggggcctg ggcggggagg
      121 ctcggctgga gggcttgggt gggggtgttg gcggctgtgt tattgttttt catgactgag
      181 cttttgcgcc gttgcgagcc gttttgttgg ctgccggggg cgacagggta aatgccagcc
      241 cggtgccaca aagcaatggc attgaaattc agttgattat attttgggga gcgagtggga
      301 ccgtccgctt ttgactaagc gggtgtccgg tttgggatgg gcggtgttga aaaaggcagg
      361 aggatctcgc ggaggatgcg gttgacggtg tcggcgcggt agagcaggaa catatgtccg
      421 gccttccgga tggtatgggt gggggtggtg agatggatcg ggaagacctc gtcccttgta
      481 ccgtgaatat gatagagcgg ttgggggatc ctggtgttct cccattcgag cacggcggtg
      541 agggcccagc ggatgaaggt gtcgtcgccg tcgcggatga tgctcatcat caattgccgg
      601 ttggcggcgg gacgcatcat gagcgaatgt tttaccgttg tggcggcctt taataaagag
      661 gcggggatca gtttgccgag gcccaggtta ccggccatgc ggtagaagcg cgggagatga
      721 gccgagacgg ggacgctgct gatgaggatg gtgcagagcg gcgtgatatg ttttgccatt
      781 tcgacggaca tgatgccgcc gagggatagt ccgacaaggg cgaagggctg ggttgtatcg
      841 atctgggctg tgaggcggat ggcgtaatcc gcgagttttt cgttcggcgc cggggggatc
      901 caatgaacgt atgctgcttc gaatccttcg ggtagacgga gatgggtgaa caaccggtag
      961 tctgcgccga tgccggagat aaaataaact ttcacagtcc gaatttatag tctacaagtt
     1021 acgatatttt tggccatgag ccgcaaggaa atcatcccgg agaccttgta cgaactgtat
     1081 agatggctgg gtctgccatt ggatctgctc gatcccaggg aggagttcac tatccacaat
     1141 gtacgggacc tgctgaccga attgccctat acttcaccga gttaccggcc taattttttt
     1201 aatttcttat ttatcaagga tgcgcgcggc cggtatacga tcgacgagat ggcgttcgat
     1261 ctcgagccgg gtacgatcta ttttaccaac ccgggtgtgt accgtacgtt tggctggcgg
     1321 gagatcgggg aagcctacct gatcaccttc aatgaaacct atctgaagga gtatgtccat
     1381 ccggagatct atgcggagtt tccgttcctg ctgacggaga ccatccggcc gttgcggctg
     1441 gacccggccg cttttaccga attcgaacgt ttgtacacgc agatcttcga ggagcaaaga
     1501 ggggattcgc cgtataagaa gaagatcatc ggtaacctgt tcgtcatatt gttgctaaag
     1561 ataaaggcgc ggttctggaa ggattacaat ccgatctatg aagggaacag gggatcgcag
     1621 attgtgaagt ctttcaggcg tgacctggag aagcattttc gcgaccttat tcatggaaag
     1681 gcggagaagg tgttccgggc cagcgagtac gccgccctgc aaagtctgca tcccagttac
     1741 ctgaataatg tgatcaagag caagacgggc aaacctatca cggtgtggat tgcagagaag
     1801 acggtatccg gggctaagtc gcttttacag cagccgggga tgtccatcaa ggagatagcg
     1861 tatacgctgg ggtttgccga gtccacgcat ttcagtgcgt tcttcaggaa acataccggg
     1921 cattcgccgg cggattaccg gaagcagttt gtgaaggcgt gagggggcgg cggggtacgc
     1981 gggggcgggg gatgttcggc ggggaagcgt gttggtgagg
//
LOCUS       DATGFG010000254         5860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1293800, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000254 DATGFG010000000
VERSION     DATGFG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293800"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VKQ52_04720"
     CDS             <1..928
                     /locus_tag="VKQ52_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86513.1"
                     /translation="RNFAKRNDGLASTHAFVASPEIVTAFAIAGHLSFNPLKDKLKNE
                     KGEEVMLDEPKGVELPPKGFSVEDAGYQAPAKDGKNVQVVVKSDSQRLQLLAPFAAWE
                     GTDLKGLKLLIKAKGKCTTDHISMAGPWLKFRGHLDNISNNMLIGAVNFFNEKTDSVK
                     NELTGEFGPVPATARAYKAAGIGSVVVGDENYGEGSSREHAAMEPRHLGVRAIVVRSF
                     ARIHETNLKKQGMLALTFANKEDYDKVLEDDSIDIVGLTSFAPGKPLTMVLNHTNGSK
                     DEVSLNHTYNEPQIAWFKAGGALNVIRAQVAK"
     gene            1052..2155
                     /locus_tag="VKQ52_04725"
     CDS             1052..2155
                     /locus_tag="VKQ52_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HLZ86514.1"
                     /translation="MTRLLAFLLGLVLISYNGDAQRLAVIGYYAGRSTAIDSFPTEKL
                     THIIFSFCHLKGNRLDANNARDTATIQGLVALKQKHPGLKVILSLGGWGGCKTCPDVF
                     ATDSGRQEFARSVRELTDYFHTDGIDLDWEYPALENVPGYPYTPQDKDNFTAVLRSLR
                     KALGRKSEISFAAGGFTSYLQTSIDWKQVAPLVDYINLMSYDLINGYSTTTGHHTGLY
                     STPQQVESVDHAVRWLDSAGVPLKKVAIGMAFYARLFQGADSINNGLYRPCHFYYGVS
                     YRDQATLLSADSGYVYHWDDVAKAPYKYNARQQLFATFDDTLSIRLKTEYALRKGLGG
                     VMFWQMTDDNFFSGGLLDVIDNTKMSNLKGRKK"
     gene            2166..3584
                     /locus_tag="VKQ52_04730"
     CDS             2166..3584
                     /locus_tag="VKQ52_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HLZ86515.1"
                     /translation="MRVSFPFRFFLLVAVAGAGACSPAKKLTPPAVTVMVGDSLPALP
                     QSEIDLPVKVAGRPLLVAADSIFPREFLSPGWPNFLQPSCDFRYKYRFVRSGFVLRCT
                     NNRISIAMEGNYQVAGGKCLCALGKPVSPWVMGYCGFDREPMRRVDLAFSTLLNFQPD
                     YHIRTSSIVEQVKALDPCNMSIFSVDMTQQIMDSIRSSVNFFCRGFDASAIKTDIAGY
                     LKQAAARAWQKTPIGSYGYLIINPVSVRVGTLNYVRDTFAVSLGISCRPELSSDSRST
                     MSPLLPALRPGGNRNGVTLFLPANYEYSFISKLLNDSLRDKSFEYKGRTVIVKELAVR
                     GIERHKIELRIDFGGSYTGRIFLRGTPVLDTAKQTLSVPDIAYSLESRDILLRMAKTL
                     LRGKIRRTMQGNNVLDLAALLKTNMPSLNAQLNRPIMTNLYTTGELKQLRLIGLLAGE
                     KNIQAQLFIQADLAVTCTGLPR"
     gene            3709..4344
                     /locus_tag="VKQ52_04735"
     CDS             3709..4344
                     /locus_tag="VKQ52_04735"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLZ86516.1"
                     /translation="MRSYYLPLKYRTSELIAGNTQIHALIAGCRSNDRASQKELYNML
                     KDYAVKICYRYQNNLEQVEEIMNEGFIKLFKNIHQFEESRHADVLASLKGWFKRILVN
                     TCIDHYRKNASYINGQMLSEDAENIADKQETGIDILSYKEIIEAIRLLSPAYRTVFNL
                     FVIEGLTHEEIAQHLDISVGSSKSNLSKARENLRKILTKKTDQKAYVEPIR"
     gene            4325..>5860
                     /locus_tag="VKQ52_04740"
     CDS             4325..>5860
                     /locus_tag="VKQ52_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86517.1"
                     /translation="MLNLSDKELDRFSKEAAQEYEPGDVLGPRSWDKLEVRINQEFGH
                     AGWNPLHHIRRFSFYYTPALVVLLGVAYYFVRTRPSSGSSPGGKAKMTTEKTTVPIQN
                     PNTTDKATSTPATSSADAAAGDNQASNAGVRAPGVARGADGTKSAAGTTSAGDGKSAA
                     GTAGAGDGKSAAGAAGAGADKGVADAKGAAGVKGEKGAVTGAGGGVNAKPMVNAGRNT
                     AGGTSNASAGTGNGGTRMGNASARTGVVGTRAHGVAAGAGTGDSHDAGGAGLSGSRVA
                     GARGSGVNGRGRRGNNGRNDNTVAVTTPTSPGQGQMGVNGSALGATAQNRELSMSSVQ
                     RPHSLGKKGVVSDSALRAFTLKSTAPTLIKNKGIYRIDRNWQFGIQFAPDYASVNSLA
                     GDKPGSSFGLTVDYQFAPHLYISSGILASRKNYAARGEDYHAPPNFYQASGIMGNVNL
                     VKGSFYMMEIPLNLRYDFTFSGSTLIFLSAGASSYLMTTENVNVYYKGGGFPYSRQDN
                     CAPR"
BASE COUNT         1334 a         1535 c         1722 g         1269 t
ORIGIN      
        1 ccgcaacttc gccaagcgta acgatggcct ggccagcacg catgcgtttg tcgccagccc
       61 ggagatcgtg acggcttttg ccatcgcggg tcatctttcc ttcaacccgc tgaaggataa
      121 attgaagaac gagaaaggcg aagaggtaat gctcgacgaa cccaagggtg tcgagttacc
      181 gcccaaggga ttctcggtgg aagacgccgg ttaccaggcg cctgcaaagg acgggaagaa
      241 tgtacaggta gtggtgaaat ccgatagcca gcgcctgcaa ttgctggcgc cgtttgccgc
      301 atgggaaggc acggatctga aaggactgaa gttgctgatc aaggccaagg gcaagtgcac
      361 caccgaccat atctcgatgg ccggtccctg gttgaagttc cgcggtcacc tggataatat
      421 ctccaacaat atgctgatcg gcgccgtgaa cttcttcaac gaaaaaacgg attccgtaaa
      481 gaacgagctt acgggcgagt ttggccccgt cccggccacg gccagggcgt ataaggcagc
      541 tggtatcggt tcggtagtcg ttggggatga gaactatggg gaagggtctt cgcgggagca
      601 cgcggcgatg gagccccgtc acctcggtgt acgcgcgatc gttgtccgga gctttgcccg
      661 gatccacgag accaacctga agaaacaggg gatgctggcg ctcacctttg ccaacaaaga
      721 ggattatgac aaggttctgg aagatgacag catcgatatc gtcggtctga ccagcttcgc
      781 cccgggcaaa ccgttgacga tggtgttgaa ccataccaac ggcagcaagg atgaggtcag
      841 cctcaaccat acgtataatg agccgcagat cgcctggttc aaggcgggtg gcgcgctgaa
      901 tgtgatacgg gcgcaggtgg cgaaataacc tttctaccta aacgaaaaag cgtccccgga
      961 aggaaggtgt gttacctgaa ctccggggat ttttttgtaa ataatatatt acattcggct
     1021 tattttgggt acttataaaa aaaacaatcg tatgacgcga ctcctcgctt ttctcctcgg
     1081 ccttgtcctt atctcttaca acggcgatgc acagcggctg gccgttatcg gctattatgc
     1141 gggcaggagt acggccatcg atagtttccc gacggaaaaa ctgacccata tcatctttag
     1201 cttttgccac ctgaaaggca accggctgga cgcgaacaat gcgcgggata ccgccactat
     1261 ccagggcctg gtggccctga agcagaaaca tcccggtctg aaggttattc tttcccttgg
     1321 cggctggggc gggtgtaaga cctgtccgga tgtttttgcg acggacagcg ggcgacagga
     1381 gtttgcgcgc agtgtgcggg aattgaccga ttattttcat accgacggga tcgacctgga
     1441 ctgggagtac cctgcgctgg agaatgtccc cgggtatccg tatacgcccc aggacaagga
     1501 caactttacg gccgtcctgc gatcgcttcg caaggcgctt ggccggaaat cggagatcag
     1561 ctttgccgcg ggcgggttta cctcctatct ccaaacctcg atcgactgga agcaggtggc
     1621 gccgctggtc gattatatca acctgatgtc ctatgatctt atcaacggct atagtacgac
     1681 aacggggcat catacggggc tctattccac tccgcagcag gtggaatccg tcgaccatgc
     1741 ggtgcgctgg ctcgattccg ccggggtacc cctgaagaag gtggccatcg gcatggcctt
     1801 ttacgcccgg ttgttccagg gcgccgacag catcaataac ggcctttacc ggccctgtca
     1861 tttctactat ggtgtctcct accgggatca ggcgaccctg ctttcggcgg acagcggcta
     1921 tgtctatcat tgggatgatg tggcgaaggc cccttacaag tataacgccc ggcagcaact
     1981 gtttgcaacc ttcgatgaca ccttatccat ccgcctgaag acggagtatg cgctgcgcaa
     2041 ggggcttggc ggggtgatgt tctggcaaat gaccgacgac aacttttttt ccggcgggtt
     2101 gctcgacgta attgataaca ctaaaatgtc gaacttgaaa ggaagaaaaa agtaatttag
     2161 ccctgatgcg cgtctctttt ccctttcgtt ttttcctgtt ggtcgcggtg gcgggcgccg
     2221 gcgcctgctc acccgcaaaa aaactcactc ccccggcggt aacggtcatg gtcggcgaca
     2281 gtctgccggc cctgccccag tcggagatcg atctgcctgt caaggtggcc ggccggcccc
     2341 tgctggtagc ggcggactcg atcttcccac gggaattcct gtcgccgggc tggccgaact
     2401 ttctgcagcc atcctgtgat ttccggtaca aatatcgttt tgtgcggagc gggtttgtcc
     2461 ttcgctgtac caacaatcgg atcagtatcg ccatggaagg caattaccag gtggccggcg
     2521 gcaagtgtct ttgtgcgctt ggcaagccgg tgagcccctg ggtcatgggt tattgtggtt
     2581 ttgaccgcga gccgatgcgg cgggtggatc tcgcctttag caccctgttg aattttcagc
     2641 ccgactatca tatccggacc agttccatcg tcgagcaggt gaaggcattg gatccctgca
     2701 atatgtcgat cttttcggtg gacatgacgc agcagatcat ggacagtatc cggagcagcg
     2761 tgaacttctt ttgccggggt tttgacgcct cggccattaa gacggatatt gccggttacc
     2821 tgaagcaggc ggccgccagg gcctggcaaa aaacgccgat cggctcgtac ggctacctga
     2881 tcatcaatcc tgtctccgtg cgtgtcggca cgctcaacta tgtgcgcgat acctttgccg
     2941 tcagtcttgg catttcctgc cgtcccgagc tcagttccga cagcaggagt acgatgtccc
     3001 cgcttttgcc cgccctgcgt ccggggggga accgcaacgg ggtgacgctt tttctgcccg
     3061 ccaactatga atattccttt atcagcaagc tgctgaacga ctccctgcgc gacaaatcgt
     3121 tcgagtacaa ggggcggacg gtgatcgtaa aagagctggc ggtgcggggg atcgagcggc
     3181 ataagatcga actgcgcatc gactttgggg gcagctatac gggtcggatc tttctgcggg
     3241 gcaccccggt gctggatacg gcaaaacaaa cgctgagcgt tccggatatc gcttattcgc
     3301 tggagagccg ggatatcctg ctccggatgg caaaaacgct gctgcgggga aagatccggc
     3361 gtaccatgca gggtaacaac gtattggacc tcgcggcctt gctaaaaacg aatatgccct
     3421 cgctgaatgc gcagctcaac cggccgatca tgaccaatct ttacacaacg ggagagctga
     3481 aacagctcag gctgatcggg ttgctggcgg gtgaaaaaaa tatccaggcg cagctcttta
     3541 tccaggccga cctggccgtc acctgcacgg gtttaccaag ataaaatact gtctatcaaa
     3601 cgagtagttc ggagagcgca ggttcaccga cagattcgct tatttgacct caaaaaagca
     3661 accattaaca tttcatttgc gtataatgcc aattaggaaa actaagttgt gcgttcttat
     3721 tatctaccac tcaaataccg cacatccgaa ttgattgcag gaaacacgca aatacacgct
     3781 ttaattgccg gttgccgttc gaatgaccgt gcaagccaaa aagagctata caatatgctc
     3841 aaggattatg ctgttaaaat ttgttaccgc tatcaaaata accttgaaca ggtcgaggag
     3901 atcatgaatg aaggatttat caaacttttc aagaacattc atcaattcga ggagtcccgt
     3961 cacgcggatg tgctggcgtc gttgaaggga tggttcaaac gcatcctggt gaacacctgt
     4021 atcgatcatt accgcaagaa cgcctcctat atcaatggcc agatgttatc cgaagatgca
     4081 gagaatattg ccgacaagca ggagacgggt atcgacatcc tctcctacaa ggagatcatc
     4141 gaagccatcc ggctgttgtc gccggcctat cgcaccgttt tcaatttgtt tgtcatagaa
     4201 ggattgaccc atgaggaaat agcccagcat ctcgatattt ccgttggttc gtccaaatcc
     4261 aacctgtcga aagccaggga aaacctacga aagatactaa caaaaaaaac tgaccagaaa
     4321 gcttatgttg aacctatccg ataaagaatt ggaccgcttc agcaaagagg cagcccagga
     4381 atatgaaccc ggagatgtgc tgggtccgcg ttcctgggac aagctggaag tgcggataaa
     4441 ccaggagttc ggccatgcgg gctggaaccc cctgcaccat atccggcgct tttctttcta
     4501 ttatacaccg gctcttgtcg tgctgctggg ggtcgcctat tattttgtgc gaacgcggcc
     4561 ctcgtcgggt agtagtccgg gcgggaaggc aaaaatgact actgaaaaaa caacagtccc
     4621 cattcaaaat cccaatacca ccgataaggc tacttctaca cccgctacct cttctgccga
     4681 cgccgccgca ggcgacaacc aggcatcgaa tgccggggtg cgggcgccgg gtgttgcccg
     4741 gggtgccgat gggacaaaga gcgcggcggg gacgaccagt gccggtgatg gtaagagcgc
     4801 cgcagggacg gccggtgctg gagatggtaa gagcgcggcg ggggcggcgg gtgccggtgc
     4861 ggataagggt gttgccgatg caaagggtgc cgccggcgtg aagggggaga agggagccgt
     4921 taccggcgcc ggtggaggtg ttaatgccaa gccgatggtc aatgccggga ggaataccgc
     4981 gggcggcacg tcgaatgcga gcgcggggac gggtaatggg ggcacgagga tgggtaatgc
     5041 gagcgcgagg acgggtgttg tcggaacgcg ggcgcatggt gttgccgccg gagccgggac
     5101 gggggattcg catgatgccg ggggagccgg gcttagcggg tcccgggtag ccggcgcgag
     5161 aggatcgggg gtgaacggca gggggagaag aggaaataat ggcaggaacg ataatacggt
     5221 ggccgtgacg accccgacga gcccgggaca gggtcagatg ggagtaaacg gcagtgcatt
     5281 gggagccacg gcgcagaacc gggagctgag catgtcttcg gtacaacggc cgcacagcct
     5341 ggggaagaaa ggggtcgtca gcgattcagc attgcgggcc tttactctga aatccaccgc
     5401 tcctaccctt atcaaaaaca agggtattta ccggatcgac cggaactggc agttcgggat
     5461 acaattcgca ccggattatg cctccgtcaa ttcgcttgcc ggcgacaaac ccggcagcag
     5521 tttcgggctg acggtcgatt accagtttgc tccgcatttg tatatcagtt cgggtattct
     5581 cgcttcgcgg aagaattacg cggcgcgcgg cgaggattat catgcgccgc cgaatttcta
     5641 ccaggcctcc ggtattatgg gtaatgtgaa tttggtaaag gggtcttttt atatgatgga
     5701 gatcccgctg aacctgcggt atgatttcac cttctcgggc agcaccctta tctttctcag
     5761 tgccggggct tcgtcctacc tgatgacgac cgagaatgtc aacgtctact ataaaggcgg
     5821 cggattcccc tatagccgcc aggacaattg tgctcccagg
//
LOCUS       DATGFG010000255         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1618, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000255 DATGFG010000000
VERSION     DATGFG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VKQ52_04745"
     CDS             <1..257
                     /locus_tag="VKQ52_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86518.1"
                     /translation="EIIRCCGVYSGVGVDFLKTSTGYAEKGATEHAVRLMRAHLPAGI
                     RIKASGGIRTATLAQQLIDAGADRLGCSASVEIVKGAQAQ"
     gene            338..757
                     /locus_tag="VKQ52_04750"
     CDS             338..757
                     /locus_tag="VKQ52_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HLZ86519.1"
                     /translation="MHRIVFIGLLFITLRGLAQTDSVVVTRDPRVAALVQKQIEINEL
                     TSREARRNVPGFRIQVMNSPDRNKVYAAKVKVYEQFPDLKPYLFYQAPNYKLKVGNFK
                     TQEEADQALQQLSRLFPSGLFVIRDIIEIKLTDTPNP"
     gene            779..1966
                     /locus_tag="VKQ52_04755"
     CDS             779..1966
                     /locus_tag="VKQ52_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HLZ86520.1"
                     /translation="MLKENIRQLAKKYAPDFIAIRHHLHAHPELSYQEFQTSAFVQQQ
                     LTRMGIPFRVMAGTGVVGMLEGKDPGSRVVALRADMDALPILEANDIPYKSTNEGVMH
                     ACGHDVHTTVLLGASRVLKELRDQWEGTIKLIFQPGEEKNPGGASLMIKEGVLLDPAP
                     QAIFGLHVHPGLETGKLSFRGGMVMASADEIYITIRGKGGHAAAPHLTVDTILVASHL
                     VVALQQIISRNRNPLSPSVLSITSVQGGHTTNVIPAEVKLMGTFRAMDEEWRFRAHEL
                     IRKQATELVHSMGAEIDLHIDVGYPMVHNNEALDRVARAEAREFLGADRVLETEVRMG
                     AEDFGYYTQRIPGCFYRLGVMNVEKGITSGVHTPTFNIDEAAIEIGVGMMAWLGAHTV
                     FGK"
     gene            complement(1981..2817)
                     /locus_tag="VKQ52_04760"
     CDS             complement(1981..2817)
                     /locus_tag="VKQ52_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86521.1"
                     /translation="MPAIVNFFHVGRTATICTCLVFLLGRSDAAADPGPSFYVDPKDT
                     AEWRLLRDSIRQKKEGLEQELRERWNSPSIEQIPEYNELQQRITRLNAVLKNLENLKK
                     SKQHYVLKNLEGEPGQVSEGGTVYSVVDNVPTIEFRIGRMNVGSFIHETTHGGQFEKK
                     ELVFDKTNGIAIGDDLDDEIEAYKAEYAYNTAELDPLISVSVPRCFDDITASWILGLQ
                     TKKGEYLFSLTKEDTAGTKIGLLPVNLNIDLKKLRRAFPCCDVGNSDRPLKNAEAYYY
                     KQ"
BASE COUNT          701 a          761 c          960 g          721 t
ORIGIN      
        1 aggagatcat ccggtgttgc ggggtctaca gcggagtggg ggtggatttc ctgaagacct
       61 ccacgggata tgcggagaag ggtgcgaccg agcatgctgt gcggttgatg cgggcgcatc
      121 tgccggcggg gatcaggatc aaggcatcgg gtgggatccg gacggcgacc ctggcacagc
      181 aattgatcga tgcaggagcg gaccgcctgg ggtgcagcgc gagtgtggaa attgtaaagg
      241 gggcccaagc gcagtgaagg gcccgaacga ggagggccgt gacgaagcag gcccgacgat
      301 gttctgccgc ggccggcgac tattaaatat gaccattatg cataggatag tatttatcgg
      361 cctgttattc attaccctgc ggggattggc gcaaacggat tcggtggtgg tgacccgcga
      421 cccgcgggtg gctgcattgg tacaaaagca gatcgagatc aatgaattga cctcgaggga
      481 ggcccggcgg aatgtgccgg ggttccggat acaggtgatg aactcccccg acaggaacaa
      541 ggtctatgcg gcgaaggtca aggtctacga acagtttccc gatctgaaac cctatctttt
      601 ttaccaggcg cccaattata agttaaaagt cggcaatttc aagacccagg aagaagcgga
      661 tcaggccctg cagcagttgt cgcggctgtt cccctcgggg ctttttgtca tcagggacat
      721 catcgaaata aagttgaccg acacacccaa tccctgatgg cgtatttaaa tccagtctat
      781 gctaaaagag aatatccggc aactcgccaa gaaatatgct cctgatttta tcgccatccg
      841 tcatcacctg catgcgcacc ccgagctctc ctaccaggaa ttccagactt cggcctttgt
      901 acaacagcaa ttgacgcgaa tgggtattcc cttccgggtg atggccggga caggggtggt
      961 gggtatgctg gagggaaagg accccggcag ccgggttgtc gccctgcggg cggatatgga
     1021 tgcgctgccg atcctggagg ccaatgatat cccctataag tcaaccaacg aaggggtgat
     1081 gcacgcctgt ggacatgatg tgcacaccac cgttctgctc ggggcgtcca gggtcttgaa
     1141 ggaattaagg gaccagtggg agggtacgat aaaactgatc ttccagccgg gcgaagagaa
     1201 gaacccgggg ggcgcgagcc tgatgataaa ggaaggggtg ttactggatc ccgcgccgca
     1261 ggcgatcttt ggactgcatg tgcatccggg tctggagacg gggaagctca gtttccgcgg
     1321 cgggatggtg atggccagcg ccgacgagat ctatattacg atccggggca agggcggtca
     1381 tgccgccgcg ccgcatctga cggtggacac gatccttgtc gcatcccacc tggtggtggc
     1441 cttgcagcag atcatcagtc gtaaccggaa ccccttgtct ccatcggtgt tatcgatcac
     1501 gtccgtccag ggcggccata cgaccaatgt catccccgcc gaagtgaagc tgatggggac
     1561 attccgggcg atggacgagg agtggcggtt cagggcgcat gaactgatcc gcaagcaggc
     1621 gaccgaactg gtgcacagta tgggagcgga gatcgacctg catatcgatg tcggttatcc
     1681 catggttcat aataacgagg cgctggaccg ggtggcaagg gcggaggccc gggaattcct
     1741 cggcgcggac cgggtgctgg aaacggaggt ccggatgggg gcggaggatt ttggttatta
     1801 tacgcaacgg atcccgggtt gtttctaccg gcttggggta atgaatgtgg agaagggcat
     1861 cacttccggt gtgcatacgc ctactttcaa catcgatgag gccgctatcg agatcggggt
     1921 ggggatgatg gcatggctgg gggcgcatac cgtattcggc aaatgacgtc actgaagcgc
     1981 tcattgctta taataatagg cttccgcatt ttttagtggc cgatcgctat tgccaacgtc
     2041 acagcaggga aatgcccttc gcagtttttt caggtctatg ttgagattca ccggtaggag
     2101 accgatcttg gtccccgccg tatcctcttt tgtaagcgaa aaaagatatt ctcctttttt
     2161 ggtttgtaaa cccaatatcc aggatgccgt aatgtcgtcg aagcatctgg ggacggacac
     2221 ggagatcagt gggtccaatt cggccgtatt gtacgcatat tccgctttgt atgcttcgat
     2281 ctcatcatcc agatcgtcgc caatggcgat cccgttggtc ttgtcaaaga ccagttcctt
     2341 tttttcgaac tggccgccat gggtggtctc atggataaaa ctgcctacat tcattctgcc
     2401 gatccgaaat tcgatggtag gaacgttgtc tacgacacta taaacggtgc ctccctcgga
     2461 gacctgaccc ggctcgcctt cgaggttctt caatacgtaa tgttgtttgc tttttttcag
     2521 gttctcgagg ttcttcaata cggcgttcag gcgggtgatc cgttgctgta gctcgttgta
     2581 ttcggggatt tgctctatcg acgggctgtt ccaccgctcg cgcagttcct gttcgaggcc
     2641 ttcttttttt tgacggatac tgtctcttaa cagtctccat tccgcggtat cctttggatc
     2701 gacgtagaac gagggtcccg gatcggcagc agcatccgat ctgcccagta ggaatacaag
     2761 gcatgtgcat atggtagcag ttctgcccac gtggaaaaag ttcacgatag caggcataag
     2821 gtcgacattt ggttttttat atggcaggct cgcggcgata cggaccaaat gggcgtaaaa
     2881 aagggacaaa tcgggatata ggtatctgaa cgacagacgc ttaagggtaa acttataaaa
     2941 gctgtgcgca ttcgtactgc agtcgtgccg attttcgcaa aaggggtgat tttacgggga
     3001 cccggggagg gcctggatgg cgccgcgggt agtggggtaa taaccgcggg gagccgggta
     3061 aaaatgggta aaagggtgtt atgcagcatt ttcgtatttt gcaccggtaa ccatgtccgt
     3121 aaatacctgt aatgcaaaaa aaa
//
LOCUS       DATGFG010000256         6480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1064819, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000256 DATGFG010000000
VERSION     DATGFG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064819"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..1309
                     /locus_tag="VKQ52_04765"
     CDS             284..1309
                     /locus_tag="VKQ52_04765"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLZ86522.1"
                     /translation="MGLFNFLTQEIAMDLGTANTLIIHNDEIVVNEPSIVALDRNNPK
                     NVLAVGKRALMMHEKTHESIRTVRPLKDGVIADFNAAELMIREMIKMIYPKKPLFPPS
                     WRMMICIPSSITEVEKRAVRDSAEQAGAKEVYLIHEPMAAALGIGIDVEEPVGNMIID
                     IGGGTTGITVIALAGIVCDQSIRIAGDEFTADIMEALRRYHSLLIGERTAEQIKIQIG
                     AALKDLDNPPDDIPVNGRDLVTGIPKQIMVSYQEIAEALDKSIFKIEEAILKALETTP
                     PELAGDIYRRGLYLTGGGALLRGLDKRLSQKIKLPVHIADDPLKSVVRGTGIALKNYD
                     RYPFVMR"
     gene            1362..2213
                     /gene="mreC"
                     /locus_tag="VKQ52_04770"
     CDS             1362..2213
                     /gene="mreC"
                     /locus_tag="VKQ52_04770"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HLZ86523.1"
                     /translation="MRNVFLFIRRHFNFLFFLVLQIIALSFLFRFNKFHEAAFLNVST
                     EITGRVNERYNNIEYYFQLKKTNESLVQENLRLRQQLKENYEAPDSIRRLIHDTIRVD
                     SNSVIKYMILEAKVVSNTTSQQANYLTIHRGFAQGVRPNMGVTGPQGIVGSVVNVSKN
                     FATVQSMLHYQFRVVAKLKHSNETGTIEWDGQSPLYVTLKNIPRSVRVEKGDTVLTTQ
                     VSSLFPANLMVGTVSEIVPDNTSNFYTLKVRPATNFGNIEYVYVIDNLQYDEQRMLED
                     STRKKIQ"
     gene            2210..2743
                     /locus_tag="VKQ52_04775"
     CDS             2210..2743
                     /locus_tag="VKQ52_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86524.1"
                     /translation="MSELLKNTIRFILFILVQVFILFKMPPLHRFVTPYLYYLYILWL
                     PFSLSRASLTFIGFFYGLCLDYFTKTPGLHAAACTLIAYARPFVISILISQEGADKNY
                     VAPSIVSMGWAPYATYVIVLTLLHHTYLVLLEWMNFGSFMYFLGKVVASTAISLLLIL
                     LTELLFFRKQKFRTNTI"
     gene            2745..5009
                     /gene="mrdA"
                     /locus_tag="VKQ52_04780"
     CDS             2745..5009
                     /gene="mrdA"
                     /locus_tag="VKQ52_04780"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLZ86525.1"
                     /translation="MPVFNQSRSNIIRLIFAGMFLIIIAQLFHLQIVSKKYKQQAQEN
                     ALFRKSVYPTRGIIYDRKGRAILNNTILYDLMVTPSQVKNIDTAYFCQLVGIDTAEFR
                     RRIVDARIKNGPFRPTTFEDLLPPEMYARMEENIWKFPGFNLQERPVRIYPYYAAAHI
                     MGYVGEVDSAILRRSNNFYQLGDYVGRSGLEQSYEKVLMGQRGTEFWIKDNKNRLVGR
                     YQDKTLDTPAVAGKNLHTYLDIELQQLAERLLFGKTGAVVAIEPSTGGILAMASGPSF
                     DPNSLTGPEKQSNYARLALDASGPLLNRAIKGQYQPGSTYKPVGALIGLDENVITPAS
                     GINCLGHYDACAPKTVKCLETWAGHAANLRLAIAHSCNSFFCNAFRLEIDNPEFRSHR
                     LGLMKWKEYANAFGLGHRLGVDLPSEDGGNIPDTTVYDKDYKRSWNSCSMVTMGIGQD
                     KMLVTPLQMANAMCIVANKGFYYTPHFVKSIEGAPEDDTLLSRFHVRHEPVTHIPDAA
                     YEVVHSGMQDVVEIGTAKTARVPGIDMCAKTGTAENKRVIDGRLVKLNNHSVFVCFAP
                     RESPKIAVAVIVENGGYGAAQAGQIASLLVEKYLNDTISTARLALEDEITSRNLMPGY
                     LVGVQFRDDSTRAALWAAQSGDSSRLLKYQTPAFRYMMRDSSYGSKSPLYQALHKPSP
                     YKSALAERLAKQRAQAAAATIGLDSAIKARAGGSDTNRPRPPRPPHKDSATGPPVRRT
                     LPNPDSVKQKDSTP"
     gene            5021..6370
                     /gene="rodA"
                     /locus_tag="VKQ52_04785"
     CDS             5021..6370
                     /gene="rodA"
                     /locus_tag="VKQ52_04785"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HLZ86526.1"
                     /translation="MSQRNPTISKGVDWSLVWIWFTLCAIGITSIFAASYHEGDNVVQ
                     GFISLKSDYSRQLLFFVLSGIIGTFILLTDSKFFTATANLSYALGIFLLLLVFPFHSR
                     VKGTESIIKFGTAFQIQPADLCKLFVNLALAKYLSRVETNFTRPRSQLIASVIALVPA
                     MFSIMQHEMGLSLVYFSFFLVMFREGLPPSVLIIGFSFSVLVVATLLLEPNTLALILT
                     GIALAAIFFLRKAIKRNRSLLFLIAGIWFVCVGVQRFVVPFAFDHVFQPYQVKRIYDA
                     IGKDYVPKDQATEAALETEQRRSGKKGNDNYNVKQSKIAIGSGGLIGKGLLKGTQTRY
                     DFVPEQKTDFIFCTIGEGFGFIGSVLFLGIYIVLLMRIVTIAERQRSVFSRCYAYGVA
                     SVLFFHIIINVCMTVGLAPVIGITLPFISYGGTSLITFSVMLFILVRLDADRQMVLR"
BASE COUNT         1440 a         1914 c         1673 g         1453 t
ORIGIN      
        1 tgtcgcccga aatgcagcgc cggcttgggt ttgtccgcgc gtaggcggga gccggaggaa
       61 gcggttcggc gaagttctaa acagccccgg cgatgttctg gctcgcagcc agacaataat
      121 taccattaac ggatacgatc agcaactttt tatcgttttg ggtttatccg catcatatgg
      181 ggaaaataat ccatcgaatc aagcgtttcg ccttgttaat tcagatattt tccttcaatt
      241 ttgctggcaa tatccttata atcgaaaatc tcgacctttt aaaatgggtt tattcaattt
      301 ccttacgcaa gaaatagcca tggacctggg taccgccaat accctgatca tacataacga
      361 cgaaatagtg gtgaatgaac cgagtattgt tgctctggat cgtaataacc ccaaaaacgt
      421 gctggccgtt ggcaaacgcg ccctgatgat gcacgaaaaa acccacgaaa gcattcgcac
      481 cgtccgaccc ctgaaagacg gggttatcgc cgacttcaac gcggcggagc tgatgatacg
      541 ggaaatgatc aagatgatct atcccaagaa acccctcttt ccccccagtt ggcggatgat
      601 gatctgtatc ccctccagta tcacggaggt ggagaaaagg gccgtccgcg acagcgccga
      661 gcaggccggg gccaaggagg tctatcttat ccacgaaccg atggccgccg ccctgggtat
      721 cggcatcgat gtggaagaac ccgtcggtaa catgatcatc gacatcggcg gtggcaccac
      781 cggtatcacg gtcatcgcgc ttgccggcat cgtctgcgac cagtccatcc gcatcgccgg
      841 cgacgaattc accgccgaca tcatggaagc cctccgccgc tatcactccc tgctgatcgg
      901 cgagcgtacg gccgaacaga tcaagatcca gatcggcgcc gccctgaagg atctcgacaa
      961 tccgccggac gatatcccgg tgaatggtcg tgacctggtg accgggatcc ccaagcagat
     1021 catggtgtct taccaggaaa tagccgaagc cctcgataag agtattttca agatagaaga
     1081 agccatcctc aaggccctgg agacgacgcc gcccgagctg gcgggggaca tctaccgccg
     1141 cggcctttac ctcaccggtg ggggcgccct gctgcggggg ctggacaagc ggctcagcca
     1201 aaagataaaa ttacccgtgc atatcgccga tgacccactg aaaagcgtgg taaggggaac
     1261 tggcattgcc ctgaagaact atgatcgcta tccgtttgtt atgagataag gaaccgagag
     1321 cctgccagca actacaaaaa ctacacttta atcagattcc ggtgcgtaac gtttttctct
     1381 tcatcagaag gcattttaac ttcctcttct tcctggtgtt gcagataata gccctttcgt
     1441 tcctcttccg gttcaacaaa ttccacgagg ccgcctttct gaacgtcagc accgagatca
     1501 ccggccgcgt caacgagcgc tacaataata tagaatacta tttccagctg aagaagacaa
     1561 acgagtcgct ggtacaggaa aacctccggc ttcgtcagca gctgaaggag aactatgagg
     1621 cgcccgactc catccgccgc ctgatccatg acaccatccg ggtggactcc aattcggtga
     1681 tcaagtatat gatcctggag gcgaaggtgg tgagcaatac cacctcccag caggccaact
     1741 accttaccat ccatcgcggt tttgcccagg gggtccgtcc gaatatgggg gtaacgggtc
     1801 cacagggcat cgtcgggtct gtcgtgaacg tcagcaagaa cttcgccacc gtccagagta
     1861 tgctccatta ccagttccgg gtggtagcca agctaaagca cagcaacgaa acgggtacga
     1921 tcgaatggga tgggcagagc cccctgtatg tcaccctcaa gaatatcccc aggagcgtca
     1981 gggtcgaaaa aggggatacg gtactgacca cccaggtctc ttcccttttc cccgccaacc
     2041 tgatggtcgg caccgtttcc gagatcgtgc ccgacaacac ctccaatttc tacaccctga
     2101 aagtcaggcc ggccaccaat ttcggcaata tcgaatatgt atatgtgatt gacaaccttc
     2161 aatatgacga gcaacgaatg ctggaggatt cgacccgcaa aaagattcaa tgagtgaact
     2221 gctgaaaaat accatccgat tcatcctgtt catcctggtg caggtgttca tccttttcaa
     2281 gatgccgccc ctccaccgct ttgtcactcc ttatctctac tacctttata ttctctggct
     2341 cccctttagc ctttcgcggg cctcgctgac ctttatcggc ttcttttacg gtctttgtct
     2401 cgactatttt accaagacac ccggcctgca cgccgccgcc tgcacgctta tcgcctatgc
     2461 ccgtcctttt gtcatcagta ttcttatctc gcaggagggc gccgacaaga actatgtcgc
     2521 cccttcgatc gtcagtatgg gatgggcccc ctatgccacc tatgtgatcg tgttgacctt
     2581 gttgcatcac acgtaccttg tgctactgga atggatgaat ttcggcagct ttatgtattt
     2641 cctgggaaaa gttgtcgcct cgacggcgat cagtcttttg ctcatcctat tgactgaatt
     2701 attgttcttc cggaagcaaa agttcaggac caatacgatc taatatgcct gtattcaatc
     2761 aatcccgtag caatatcatc cggctcatct ttgccggcat gttcctgatc atcattgccc
     2821 agttattcca cctgcagatc gtttccaaaa agtacaagca gcaggcccag gagaatgcgc
     2881 tttttcggaa aagcgtgtat cccacccggg ggatcatcta tgaccggaag gggcgcgcca
     2941 tcctcaacaa taccatcctc tatgacctga tggtcacgcc ctcccaggtg aagaatatcg
     3001 acaccgctta tttctgtcag ctggtgggca tcgatacggc cgagtttcgc cggcggatcg
     3061 tcgacgcccg gataaagaac ggccccttcc gccctaccac cttcgaggac ctgctgccgc
     3121 cggagatgta tgcccgcatg gaagagaata tctggaaatt ccccgggttc aacctgcagg
     3181 aacgtcctgt ccggatctat ccctattatg ccgccgcgca tatcatgggc tatgtcgggg
     3241 aggtggactc cgccatcctg cgccggagca ataattttta ccaattaggg gactatgtcg
     3301 gtcggagcgg tctcgaacaa tcctatgaga aagtgctcat ggggcagcgg gggaccgagt
     3361 tctggatcaa ggataacaag aaccggctgg tcggccgtta ccaggacaag accctggata
     3421 cccccgccgt cgccgggaag aacctgcaca cctatctcga catcgagctg cagcagctgg
     3481 ccgagcgcct gctgttcggc aagacggggg ccgtcgtcgc cattgaaccg tccacgggtg
     3541 gtatcctggc catggcttcc ggtcccagct tcgaccctaa ttcccttacc ggtccggaga
     3601 agcaatccaa ctatgcgcgg ctggcgctcg acgcttccgg ccccctgctc aaccgcgcca
     3661 tcaaaggaca ataccaaccc ggttccacct acaagcctgt cggcgcgctg atcggcctgg
     3721 acgagaacgt gatcaccccg gcgagcggca tcaactgcct gggccattac gatgcctgcg
     3781 cccctaaaac cgttaaatgc ctggagacct gggccggcca tgccgcgaac cttcgcctcg
     3841 ccatcgccca ctcctgcaat tccttttttt gcaacgcctt tcgcctcgag atcgataatc
     3901 ccgaattccg cagccaccgc ctcgggctga tgaaatggaa ggaatatgcc aatgcgttcg
     3961 gcctcggtca ccgcctcggt gtggatcttc ccagcgaaga cggcgggaat atccctgata
     4021 cgacagtgta tgacaaagac tataagcgga gctggaactc ctgctcgatg gtgacgatgg
     4081 gcatcggcca ggacaagatg ctcgttacgc ccctgcagat ggccaatgcg atgtgcatcg
     4141 tggccaacaa agggttttac tatacacccc atttcgtaaa aagcatcgaa ggggcgcccg
     4201 aagacgacac cttgctcagc cgctttcatg tgcggcacga gccggtgacg catatccccg
     4261 atgcggcgta tgaagtggtc catagtggaa tgcaggatgt ggtggagatc ggtaccgcca
     4321 agacggcccg tgtcccgggt atcgacatgt gcgccaagac gggtacggcg gagaacaaac
     4381 gggtcatcga cggccggctc gtcaagctca acaaccactc cgtcttcgtt tgttttgcgc
     4441 cccgcgaaag cccgaagatc gccgtcgccg tcatcgtcga gaatggcggt tatggcgcgg
     4501 cccaggcagg gcagatcgct tccctgctgg tagaaaaata tctcaacgac accatctcca
     4561 ccgcaaggct ggcattggag gacgagatca cgagtcgtaa cctgatgccc ggctacctgg
     4621 tcggggtgca attcagggac gactccacgc gcgccgcttt atgggccgca cagtccggcg
     4681 acagctcccg gttgctaaaa taccagacac ccgcattccg gtacatgatg cgggattcct
     4741 cctacggttc gaaaagcccg ttgtaccagg ctttgcacaa gccctcgccg tataagagcg
     4801 cccttgccga acggctggcg aagcaacggg cgcaggcggc ggccgcgacc atcgggctgg
     4861 attcggcgat aaaggcgagg gcgggtggct ccgacacgaa ccggccgagg cctccccgtc
     4921 cgccgcataa ggattccgct acgggcccgc ccgtccgccg gacgttaccc aaccctgatt
     4981 ccgtcaaaca aaaagactcg accccgtaat gcaataaatc atgagccaaa gaaatcccac
     5041 tatatcgaaa ggcgtcgact ggtccctggt ctggatctgg ttcaccctgt gcgccatcgg
     5101 gatcaccagc atctttgccg cctcctatca tgaaggggat aatgtcgttc agggctttat
     5161 ctccctgaag agcgactaca gccggcaatt gctgttcttt gtcctctccg ggatcatcgg
     5221 aacgttcatc ctgctcaccg acagtaaatt ctttacggcc acggccaacc tgagctatgc
     5281 actgggcatc ttcctgctgc tgctggtctt tccctttcac tcgcgtgtca agggaacgga
     5341 gtcgatcatc aagtttggca ccgcgttcca gatccagccc gccgacctct gtaaactgtt
     5401 cgtcaatctt gcgcttgcaa aatacctgtc gcgggtggag accaatttta cccggccgcg
     5461 cagccagctt atcgcctcgg tgatcgcgct tgttcccgcg atgttcagca tcatgcagca
     5521 tgaaatgggg ctttccctgg tgtatttttc cttctttctc gtcatgttcc gggaaggtct
     5581 tcctccttcg gtgctgatca tcggcttttc cttttcggtg ctggtggtcg ccaccctcct
     5641 gctggagccc aataccctcg cgctgatcct gacgggtatt gccctggccg cgatcttctt
     5701 cctccggaag gcgatcaagc ggaaccggag tcttcttttc ctgattgccg gcatctggtt
     5761 cgtctgcgtc ggggtgcagc ggtttgtcgt accgtttgcc ttcgaccatg tctttcagcc
     5821 ctaccaggtg aagcggatct atgacgccat cggtaaagac tatgttccca aggaccaggc
     5881 caccgaagcg gcgctggaga ccgaacagcg gagaagcggg aaaaaaggga atgacaacta
     5941 caacgtcaag cagagcaaga tcgccatcgg tagcggcggt ctcatcggta agggcctgct
     6001 caagggtacc cagacccgtt atgatttcgt gcccgagcaa aaaacggatt tcatcttctg
     6061 taccatcggc gaaggctttg gttttatcgg gagcgtgctg ttcctgggca tctatattgt
     6121 gctgctgatg cggatcgtca ccattgccga acgacagcga agcgtcttca gccgatgtta
     6181 cgcgtatggt gtggccagtg tgctcttctt ccatatcatc atcaacgtct gtatgaccgt
     6241 gggacttgcg cctgtcatcg ggatcacgct gccctttatc agctatgggg ggacatccct
     6301 catcacgttc agcgtcatgc tcttcatcct cgtccggctg gatgccgacc gccagatggt
     6361 gttgcgatag accgcccccc ttaatgcatt atctttgtgc ccagtcaacg tactattcta
     6421 tacgcatgca aatcattccg ctatccgaag gagcttttac gatagataag accaagcgat
//
LOCUS       DATGFG010000257         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1523270, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000257 DATGFG010000000
VERSION     DATGFG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523270"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VKQ52_04790"
     CDS             complement(<1..954)
                     /locus_tag="VKQ52_04790"
                     /inference="COORDINATES: protein motif:HMM:NF018009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HLZ86527.1"
                     /translation="MAKQAINKEYKVFLEEIKRKVYEAQYQAMRKVNKELIGLYWYIG
                     ESIMARQKRFKWGKAVVETLATDLQNEFPGVSGFSVQNLWYMRKFYKEYHTDEKLQPL
                     VGEIGWSHNLVIMDKCKDKLEREFYIKMTIKYGWTKNVLIHHVEGKSYERFLLGQTNF
                     DKVVPQKYKQQAKLAVRDEYNFEFLEIADEHAEKELEAAIMKNMRRFLQEMGGDFTFV
                     GNQYRLQSGDENYYIDILLYHRRLRSMVAIELKAGKFKPSYAGQMNFYLTVLNDQVKT
                     EEENASIGIIVCKAKDRTTVEYALKEANNPIGVASYKVTTSL"
     gene            1287..2177
                     /locus_tag="VKQ52_04795"
     CDS             1287..2177
                     /locus_tag="VKQ52_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86528.1"
                     /translation="MLSDKQDIIRQLQEEILPLQGYRPPTRGAVVDVGLGPVAGAFPN
                     GVFPTGAVHEFLGASREDAAATGGFVAGILGRLMGIGGVCVWIGPRRTIFPPALKRFG
                     IDAERIIFIELQREKDALWAMEEALKCEGLAAVVGEIREIDLTASRKLQLAVEKSRVT
                     GFLLRHQPRNVGTVAAVARWRVGALASIPEEGMPGVGPPRWKVELLKVRNGMPGSWTI
                     EWMGGAVKHEGSAQGMWAAQPVDSSHGRDVALGMRGAQAVHGGDEAYAAQVVGSSHGG
                     QAAEGAGAGIALQKQRRKTG"
     gene            2180..>3069
                     /locus_tag="VKQ52_04800"
     CDS             2180..>3069
                     /locus_tag="VKQ52_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HLZ86529.1"
                     /translation="MNKRFVSLFFPHLKTDWLAIRRPALRGTPFVLSAKDHNRILVTA
                     ASPAAGKEGLQPGIVVADARAIVPTLTVIEDKPGLAGRLLRGIGEWCIRFTPLVGVDG
                     EDGLLLDVSGCAHLWGGEVPYLKEMLRRLRALGYEVRGAMADTMLTAWAIARFGKGYP
                     IIAPDKQAEALAPLPPAALRLEPEVTERLYQVGLTQIERFVSMPRGALRRRFGAGLLQ
                     RLDQALGFAEEVLEPVCPVAVFAERLPCLEPIQTRTGIEIALQRLLNTLCQRLQQEGK
                     GLRVAVFKGYRIDGKLEQIE"
BASE COUNT          631 a          796 c          896 g          746 t
ORIGIN      
        1 taaagacgta gttaccttgt aggaagctac tcctatcgga ttattggctt ctttcagcgc
       61 atactctacc gtcgtcctgt cttttgcttt gcaaacaata atgccgatgg aggcgttctc
      121 ttcctccgtc tttacctgat cgttcaggac ggttaagtaa aaattcatct ggccggcata
      181 cgatggtttg aactttccag ctttcagttc aatagcaacc atcgatctaa gtctccggtg
      241 gtagagcagt atatcgatat agtaattttc gtctcctgac tgcaaccggt actgattgcc
      301 aacaaacgta aaatcgccgc ccatttcctg taaaaaccgg cgcatgttct tcatgatggc
      361 ggcttccagc tctttttccg catgctcgtc cgctatttcc aggaactcaa agttgtattc
      421 atccctgacc gccagtttag cctgctgctt gtatttttgc gggacaacct tatcaaagtt
      481 cgtctgccct aataaaaatc gctcatagct tttcccttcc acgtgatgga tcagtacatt
      541 ctttgtccac ccgtatttaa tcgtcatctt gatatagaac tccctctcta gcttgtcctt
      601 acacttatcc ataatgacaa gattatgact ccaccctatt tctccaacca acggttggag
      661 tttttcatct gtatggtact ctttatagaa cttccgcata taccaaagat tctgaacaga
      721 aaaaccgctc acaccaggaa actcgttttg caagtctgtc gccaacgtct ctacaaccgc
      781 cttaccccat ttaaaccgct tctgccgggc catgatggac tcgccaatat accaatacag
      841 accgatcagc tctttattca ccttgcgcat ggcctggtat tgtgcctcgt agacctttct
      901 cttgatctcc tctaaaaaga ccttatattc tttattgatc gcttgctttg ccatacctgt
      961 aaaagtattg atttctggcg ggtggtgggt ggcggataaa aaaaccatcc ccttccgagg
     1021 aaagagatgg tacagtgtgc gtgtgcttat gaagagagaa accctgtttg tgggggatag
     1081 tattggttag tgtctgccgg ggggaggatt gtaaccgcgg gaaagggagt tttttttggt
     1141 gtcgatttgt ccttttttga ttcctgtttt ggcatttttg tgtaccgcgt ttgtcctcaa
     1201 ttggttaatg tggaaagttt aggtgggatg ggcatggaaa tactaattat tttagtattt
     1261 ttgtagtaaa cacacacttt atttgcatgc tgagcgacaa gcaggatatt atccgccagt
     1321 tgcaggagga gatccttccc ctgcagggtt accggccgcc gacgcggggg gccgtggtgg
     1381 atgtgggtct gggaccagtg gcgggtgctt ttccgaacgg cgttttccca acgggggcgg
     1441 tgcatgagtt ccttggcgcg tcgcgggagg atgcggcggc aaccggcggg tttgtggcgg
     1501 gcatcctggg aagactgatg ggcatcggcg gggtgtgtgt ttggatcggc ccccggcgca
     1561 cgatctttcc cccggccctg aagcggtttg gaatagacgc cgaacggatc atctttatcg
     1621 agttgcaacg tgagaaggat gccctctggg ccatggaaga agccttgaaa tgcgaggggt
     1681 tggcggcagt tgtgggtgag atccgagaga tcgatcttac cgcctcacga aaattgcagt
     1741 tggccgtcga gaaaagccgc gtcaccggtt tcctgcttcg ccatcagcca aggaacgtgg
     1801 gtaccgtggc ggccgtcgcc cggtggcggg ttggcgcact ggcaagtata cccgaagaag
     1861 ggatgccggg tgtcggcccg ccccggtgga aggtggagtt attaaaggtg cgcaatggta
     1921 tgccgggcag ttggaccatc gaatggatgg ggggagcagt caagcacgag ggctccgcgc
     1981 aggggatgtg ggctgcgcag cctgtggata gtagtcatgg ccgggatgtt gcgctgggca
     2041 tgcgaggggc gcaggcggtg catggcggtg atgaagcgta tgccgcgcaa gttgtgggta
     2101 gtagtcatgg cgggcaggca gcggagggcg cgggagccgg gatagcatta cagaagcaac
     2161 gacggaaaac aggataacca tgaacaagcg ctttgtctcc cttttctttc cccacctcaa
     2221 aacggattgg ctggccatcc gccggccggc cctgcgcggc acacccttcg tgctttccgc
     2281 aaaggaccat aaccgcatac tcgttacggc cgcgagcccc gccgccggga aggagggact
     2341 gcaaccgggg atcgttgtcg ccgacgcgag agccatcgtc cccaccctga cggtgatcga
     2401 agacaaaccg gggctggccg gccgattgct gcggggcatc ggcgagtggt gcatccgctt
     2461 tacgccattg gtgggcgtcg atggagaaga cgggcttttg ttggacgtca gcggttgtgc
     2521 gcacctgtgg ggtggagaag tcccgtatct gaaagagatg ctgcgccggc tccgggcgct
     2581 gggctatgag gtccgcggcg cgatggccga cacgatgctt accgcctggg cgatcgcccg
     2641 atttgggaaa gggtatccca tcattgcacc ggacaaacag gcagaagccc tcgctcccct
     2701 accccccgcg gcactccgcc tcgaaccgga ggtaacggaa cggctttacc aggtgggtct
     2761 gacgcagatc gagagattcg tatcgatgcc cagaggggcc ctgcggcgtc gctttggcgc
     2821 gggattgttg caacgcctcg accaggccct cggctttgca gaagaggtgc tggagcccgt
     2881 gtgccccgtt gccgtttttg ccgagcggct gccctgcctg gaaccgatac agacgcgaac
     2941 cgggatcgag atcgccctcc agcgcctgct gaataccctt tgccaacgcc tgcaacagga
     3001 aggcaagggc ctgcgcgttg ctgtcttcaa aggctaccgc atcgacggga agctggaaca
     3061 gatcgagat
//
LOCUS       DATGFG010000258         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1276246, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000258 DATGFG010000000
VERSION     DATGFG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276246"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="VKQ52_04805"
     CDS             <1..1623
                     /locus_tag="VKQ52_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ86530.1"
                     /translation="TTVAKELTGVYSDYGNLYKNRNDTRKAAEYYFKSVALGEKYGIK
                     KSLLATYLNLSALFQNLGQLPDQLVYVDRALSLAKELNKPKYLFQGFLFKGHYYAETE
                     EYAEALRYLDSAGQYYSENWEFNERQTYLMTRASCYQGLKRYDSAIRYFQISYDRASR
                     GNSPWNSTQALVKLGYVYLLEKKYKPAEQYLNKALEAALKDSIVLFEKEAYESLARLY
                     SETGRHSEAYQYYKTFHALTDSLVGTEKKKFTLDLQTKYETDKKEQEIKTLQAEQQVQ
                     QLELRQKNLWNIVLVSAATLLLLLAALVYRNYRQKQRLQQQRITELETEKKLTATEAV
                     LKGEEQERARLAKDLHDGLSGMLSGAKYSLQSMEGSLVMTADNHIAFQRGMDMLDSSI
                     KEMRRVAQNMMPEALIRFGLDTALNDYCTEINKSGAVNLVYQSIGMENAHIPQSDAIA
                     VYRIVQELTNNIIKHSGASQALVQLLHEGNRLVVNVEDNGKGFDHPTFERLAGMGWRN
                     IRSRIEYLKGKYEIQTQPGKGASINVEINLTQ"
     gene            1620..2252
                     /locus_tag="VKQ52_04810"
     CDS             1620..2252
                     /locus_tag="VKQ52_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ86531.1"
                     /translation="MITVFITDDHHMVSEGIRSLLLNEKDIEFIGSASTVASCKAFLA
                     RQQPDVLLLDINLPDGNGIDLCLELRQKYPGVFILGLSTFNQESYIRKMIDNGASGYI
                     LKNATQQELTEAIHTVYSGKTYLAFDVAKTLSSAKKLPAGQFLIGRREKEVLQLITEG
                     FTSGEIAQQLGISTYTVDTYRKSLLTKLSARNTADLIRLAFLNQLVSLDK"
     gene            2327..>2669
                     /locus_tag="VKQ52_04815"
     CDS             2327..>2669
                     /locus_tag="VKQ52_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86532.1"
                     /translation="MHIKKFLVLSLWILFFNTGFAQQCTDENVAGLPGGWRAAMKGAS
                     DHSAADMAREKVLLDEIAQYIRSNFTWQPIGGDITAGNAYSIRGLDYRPTPVRKLANT
                     YSMFVFFQHFFC"
BASE COUNT          701 a          715 c          691 g          562 t
ORIGIN      
        1 acgactgtcg ccaaggaact gacgggcgtt tatagtgatt acggcaatct ttataagaac
       61 aggaatgaca cccgaaaggc ggcagagtat tatttcaagt ccgtggccct gggtgagaag
      121 tatggaatca agaaaagcct gttggccacc tacctcaatc tttcggccct tttccagaat
      181 cttggccaat tgccggacca actggtctat gtcgaccggg ccctgtctct cgcgaaagaa
      241 ctgaataagc cgaaatatct tttccaggga tttttgttta aaggccatta ctatgcggaa
      301 accgaggaat atgccgaagc cctaagatat ctcgacagcg cagggcaata ttacagcgag
      361 aactgggaat tcaacgagcg gcaaacctac ctcatgacca gggcctcctg ttaccagggg
      421 ctcaaacgat acgacagcgc cattcgttat ttccaaatca gctatgatcg ggcgagccgg
      481 ggtaattccc cctggaatag tacgcaggcc ctggtaaaac ttgggtatgt ctacctcctg
      541 gaaaagaaat acaagcccgc cgagcaatac ctgaataaag cactcgaagc ggccttgaaa
      601 gacagcatcg tattgttcga aaaagaggcc tatgaaagcc tggccaggct ttattcagag
      661 accggccggc acagtgaggc ctaccaatac tataagacct ttcacgcgct gaccgattcc
      721 ctcgtgggca cggagaaaaa gaagttcacg ctcgatctcc aaacgaaata cgaaaccgat
      781 aaaaaggagc aggagataaa gaccttgcag gccgaacagc aggttcagca actggaactc
      841 cggcaaaaga acctatggaa catcgtgctt gtttcggctg ctacgctgct gttgttgctt
      901 gcggcgctgg tttatcggaa ctaccgccaa aaacaacggc ttcagcaaca gcggataacc
      961 gaattggaga cggaaaagaa gctgactgcc accgaagccg ttctgaaggg agaggaacaa
     1021 gagcgggccc ggcttgcaaa agacctccat gacggtctca gcgggatgct ttcgggtgca
     1081 aaatattccc tgcagtccat ggagggcagc ctggtcatga ccgccgacaa tcacatcgcc
     1141 ttccagcggg ggatggacat gctggacagc tccatcaaag agatgcgccg ggtggcacag
     1201 aacatgatgc ccgaggccct gatccggttc gggctggata cggcgctgaa tgactattgc
     1261 acggagataa acaaatccgg ggccgtgaac ctggtctacc agtccatcgg aatggagaat
     1321 gcgcatattc cacagtccga cgccatcgcc gtctaccgca tcgtccagga gctgaccaat
     1381 aatatcatca aacattccgg cgcctcgcag gcgctggtgc agctgctaca cgaagggaac
     1441 cggctcgtcg tcaatgtcga ggacaatggt aagggttttg accatccaac cttcgagcga
     1501 ctggcgggga tgggctggcg gaatatccgt tcgcggatcg aatacctgaa aggaaaatac
     1561 gagatccaga cacaacccgg caaaggcgcc tccatcaatg tcgaaatcaa cctcacccaa
     1621 tgataacagt attcatcacg gacgatcatc atatggtgtc ggagggcatt cgctcgctgc
     1681 ttcttaacga gaaggatatc gagttcatcg gttctgcttc caccgtcgcc tcctgcaagg
     1741 cctttctcgc ccggcagcag ccggatgttc ttttactgga catcaatctt cccgatggta
     1801 atgggatcga tctctgcctg gagttgcggc aaaaataccc gggcgtgttt atcctcgggc
     1861 tcagcacctt caaccaggaa agttatatcc ggaaaatgat cgataacggc gcctcgggat
     1921 atatcctgaa gaacgccacc caacaggaac ttaccgaagc cattcacacc gtttatagcg
     1981 gcaagaccta tcttgccttc gatgtggcca aaaccctcag ttcggcaaaa aaacttcccg
     2041 cgggtcaatt tctcatcggc agacgcgaaa aggaggtgct tcagctgatc acggaagggt
     2101 tcaccagcgg ggagatcgct cagcaactgg ggatcagcac atatactgtc gacacctatc
     2161 gtaagagcct gttgacaaag ctgagtgccc gcaacaccgc cgatctgatc cggcttgcct
     2221 ttctgaatca actcgtctca ctggacaaat aagccactcc cttctcccta tttttaggga
     2281 ttgcccgggc gcgggccgat gaatatgttt gtataaaata gccggtatgc acatcaaaaa
     2341 attcctggtc ctgtccctct ggatcctttt tttcaataca ggttttgccc agcaatgcac
     2401 cgatgagaac gtagccggtt tgcccggggg ctggcgcgcg gctatgaagg gagccagcga
     2461 ccatagtgcc gccgatatgg cgcgggagaa ggtcctgctg gacgagatcg cgcaatatat
     2521 ccgcagcaat tttacctggc aacccatcgg cggggatatc acggccggaa atgcgtacag
     2581 catccggggg ctggattacc ggccgacacc ggtcagaaaa ctcgccaata cctacagtat
     2641 gtttgttttc ttccaacatt ttttttgca
//
LOCUS       DATGFG010000259         4108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_346663, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000259 DATGFG010000000
VERSION     DATGFG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346663"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1323)
                     /locus_tag="VKQ52_04820"
     CDS             complement(103..1323)
                     /locus_tag="VKQ52_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HLZ86533.1"
                     /translation="MNGRPRYNHLRRITVRLLAGLLVTLLFASCGGNGKQLNRRILLR
                     HTDKNPYGTHIAYEGLPWIFPDAEITINKTSPQSLYSRADKKALIFIGSRMDATPAEV
                     SSIMNFVGEGNHVFLSATWMSDTLLHTLGIRTAFRYHLYYDQMDSLTVGVFSPVNAGY
                     KRFTYPGDSYDSYITILDSQYTSVLGRDEGGRPNFVRISYKGGGAIYLHFAPLAFSNF
                     FLLHKKNIAYYENALSYIPPTVKEIIWDEYFRYGHGRNGGSFSALQYILNGNAGLRMG
                     FWLLLLLFLLIYLFDSKRRQRMVPAIAPLRNTSLDFVQTIGRLYFQRRDNRNLATKMA
                     VHFQDQVRTHYHLPVTTLDEEFVNRLSYRTGYPKEQLQELVEYMRHLPLKGYIPDEEL
                     VHFHRQLEAFYKHT"
     gene            complement(1320..2141)
                     /locus_tag="VKQ52_04825"
     CDS             complement(1320..2141)
                     /locus_tag="VKQ52_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HLZ86534.1"
                     /translation="MMRRRSGILFLFLLGSSLSLTAQRGDSAGGPRLDTTVNDTIVVG
                     VDDPIVRTHVDSSYSAGLESGGAAATTEPSPVLRTLPDTTVRRWQDDRVFAYANDPEY
                     WKIRRERPNAFSRWLSGVLLSKGFRYAVLLLLGGLLIFAIVRIAMENNLSLFYRGGRK
                     RKGSGDDTDSLLEEEDIDQRLQHFLQAGDKRQATRYLYLKTLRLLSARNLIRRHADTT
                     NQEYLRQLKDTPPEAAFRFLTGAYERVWYGDFVLSDASFRRLHDYFSDLHKTLEA"
     gene            complement(2138..3088)
                     /locus_tag="VKQ52_04830"
     CDS             complement(2138..3088)
                     /locus_tag="VKQ52_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HLZ86535.1"
                     /translation="MREGLFIKKNIDKWKQYQYEPATDPDEMAGQFTDLVNDLGYSKT
                     FYPHSKVTNYLNGLASRVYLGIYRNKKEESSRIIRFWKTELPTVVRRYHREILYSFII
                     FVLFAVMAAFSAAHDETFVRGVLGDQYVDMTEDNIAKGDPFGVYKRDDQLSMFLYIAT
                     NNIQVSFLIFIAGLIVSLGTIWQLFQNGVMVGAFQYYFFTKGLGLQSVLVIWIHGTLE
                     ISSIIISGAAGLVLGNSILFPGTHKRLDSLKRGGKDGLKLMIGLVPVFIMAAFLEGFV
                     TRYARMPVWLSVSILAGSLFFVIWYFVVYPIQLEKKKRAV"
     gene            3151..3909
                     /locus_tag="VKQ52_04835"
     CDS             3151..3909
                     /locus_tag="VKQ52_04835"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HLZ86536.1"
                     /translation="MLQVKLDTGFNIEVDFLLPTFFRRFVAWTIDFAIIFSYYLVGSN
                     LLGKTIGQGGKIWVWVLFGLPPLFYHLLCESLLNGQSVGKKAMRIKVISADGGQPSLS
                     QFLIRWLFRLTDLPWWVLGSVFVGALPWWCSVFLFAGLASILATPYSQRIGDLVAGTI
                     LIDTRNQTSWQDTVFTELSDDYKPRYPQVMQLSDKDINTLKTIINSVNRSGDYDLSMR
                     IADRIRSKLDIYSDQDSLDFLQTLLKDYNYYATR"
     gene            complement(3899..>4108)
                     /locus_tag="VKQ52_04840"
     CDS             complement(3899..>4108)
                     /locus_tag="VKQ52_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86537.1"
                     /translation="NFFPCLYLYNFATRMRVALRNNDQDQLNIAFKNMRALYRFVGVL
                     MIICLGFWVLFILILIISTAGRVNA"
BASE COUNT          926 a         1110 c         1166 g          906 t
ORIGIN      
        1 tcctgcccga cgatgacctt gccgatctcg ctcttcagtt gttccaccgc ctgtctcagc
       61 tcatcgagat ccgtccgttg ttcgaataaa ttctcttcca tgttatgtat gtttataaaa
      121 tgcttccagt tgacgatgaa aatgcaccag ctcttcgtcc gggatatagc ccttcaacgg
      181 caaatgccgc atatactcca ccaattcctg cagttgttcc ttcgggtatc ccgtccggta
      241 ggatagccgg ttcacaaatt cttcgtccag cgtcgtcacc ggcaggtgat aatgcgtgcg
      301 gacctggtcc tggaaatgga ccgccatctt cgttgcaaga ttacggttgt cccggcgctg
      361 aaaatagagc cggccgatgg tctggacaaa atccaatgag gtattgcgca gcggcgcgat
      421 ggccgggacc atccgctgac ggcgcttgga atcgaagagg tagatcagga ggaacaacag
      481 gagcaggagc cagaagccca tccgaagccc cgcattgcca ttcaggatat actgaagggc
      541 ggagaaggac cctccgttcc ggccatggcc ataccggaaa tattcgtccc agatgatctc
      601 tttcaccgtg ggcggtatat aggaaagcgc gttctcataa taggcgatgt tcttcttatg
      661 aagaaggaag aaattggaga aggcaagcgg agcaaaatga agatagatgg ccccccctcc
      721 cttatagctg atccggacga aattgggacg gccgccctca tcccggccga gaacggatgt
      781 atactgcgag tccagtatcg ttatatagga atcgtagctg tctcccggat aggtgaagcg
      841 tttatagccg gcattcacag ggctaaagac cccgaccgta aggctgtcca tctggtcata
      901 atacaggtgg taccggaagg cagtacggat acccagtgta tgcagaaggg tatcgctcat
      961 ccaggtggcg gagaggaaaa catggttgcc ttccccgaca aaattcatga tcgacgaaac
     1021 ctccgcgggg gtggcgtcca tccgggagcc gataaagatg agggctttct tgtctgcccg
     1081 tgagtacagg ctctgcgggc tggtcttgtt gatggtgatc tccgcgtcag ggaagatcca
     1141 gggcaggcct tcgtatgcga tatgggtgcc gtagggattt ttatcggtat gccgcaggag
     1201 gatccgccgg ttcagttgct tcccgttgcc cccgcaggaa gcgaaaagca gggtcaccag
     1261 caggccggcg agcaggcgca ccgttatccg gcgaaggtgg ttatatcggg gccttccgtt
     1321 catgcctcca gggttttatg aaggtctgaa aaatagtcgt gcagtcgccg gaacgatgcg
     1381 tcgctaagga cgaaatcccc ataccagacc cgctcgtacg ccccggtgag gaaccggaag
     1441 gccgcctccg gcggagtgtc cttcaactgc cgcaggtatt cctggttggt cgtatcggca
     1501 tgccggcgga tcaggttgcg ggcgctgagc aatcgcaggg tcttcagata gagataacgg
     1561 gtagcctgtc gcttgtcacc ggcctggagg aaatgctgca gccgttggtc gatgtcctct
     1621 tcttccaaca gactgtccgt gtcatcaccc gaacctttcc gcttccttcc accccggtag
     1681 aacaggctta ggttattctc catggcgatg cgcacgatag cgaagatcag gaggccgccc
     1741 agcaggagca gcaccgcata gcggaatccc ttgctcagca ggaccccgga cagccagcgg
     1801 ctaaaggcgt ttggtctttc ccgccggatc ttccagtatt ccggatcgtt ggcataggcg
     1861 aagacccggt cgtcctgcca gcgccggacc gtcgtatcgg gcagggttcg caacaccggg
     1921 gatggttccg tcgtggccgc ggcgcccccc gattcgagac ccgctgaata ggagctgtcg
     1981 acatgggtcc tgacgatggg gtcatcgaca cccacgacga tcgtatcgtt caccgtcgtg
     2041 tccaacctgg ggccgcccgc gctgtccccc cgctgtgcag ttagggaaag gctggagcca
     2101 agcaggaaaa ggaagaggat ccccgaacgg cggcgcatca tacggctctc ttttttttct
     2161 ccagttggat gggatatacg acaaaatacc agatgacgaa aaacagggaa cccgccagga
     2221 tggaaacgct cagccagacc ggcatccggg cgtagcgggt gacgaaccct tcgaggaagg
     2281 cggccatgat aaacaccggc accaggccga tcatcagttt caatccatcc ttgccgcccc
     2341 gcttgagaga atccagccgc ttgtgggtcc cggggaagag gatgctgtta ccgaggacga
     2401 ggcccgcggc gccggagatg atgatggaag agatctcgag cgtgccgtgg atccagatga
     2461 ccaggacgga ttggaggccg aggcctttcg tgaaaaagta atactgaaag gcgccgacca
     2521 tgaccccatt ctggaacagt tgccagatgg tgcccagcga gacgatgaga cccgcgataa
     2581 agatgagaaa ggacacctgg atattattgg tggcgatgta caggaacatg ctcagctggt
     2641 cgtcgcgttt ataaacgccg aaagggtccc ccttggcgat attgtcctcg gtcatgtcca
     2701 catactggtc tcccagcacc ccgcggacaa aggtctcgtc gtgcgccgcg gagaaagcag
     2761 ccatgacggc aaacaggacg aagatgataa aggaatacag tatctcccgg tgataccgtc
     2821 tcaccaccgt cggcagttcc gtcttccaga agcggatgat gcgcgaagac tcttcttttt
     2881 tattgcggta gatgccgaga taaacgcggg aggccagtcc attcagataa ttggtgactt
     2941 tgctgtgtgg atagaaggtc ttcgaatacc cgaggtcgtt cacgaggtcg gtgaattgcc
     3001 cggccatttc atcaggatca gtcgcgggct cgtattgata ctgtttccac ttatcaatat
     3061 tttttttaat aaagagacct tctctcacag gaaaaattta gtagtatatt tatccggtcg
     3121 ttacgattcg ctctcaatat agtgaattct atgttacagg tcaaactgga caccggcttc
     3181 aacatcgaag tggattttct gctccctacc ttcttcaggc gattcgtcgc ctggaccatc
     3241 gactttgcga ttattttttc ctattacctc gtagggtcga acctgctggg caagaccatt
     3301 gggcagggtg gtaagatctg ggtctgggta ttgttcgggt tgccgcccct gttctatcac
     3361 ctgctttgcg agtcgctgct caacgggcaa agcgtcggga aaaaagcgat gcggatcaag
     3421 gtcatctccg ccgacggcgg ccagccttcc ctcagtcagt tccttatccg ctggctgttc
     3481 cggctcaccg atctgccctg gtgggtgctg ggttccgtct ttgtaggcgc acttccctgg
     3541 tggtgcagcg tgttcctctt tgccgggctg gccagtatcc ttgccacgcc ctattcccag
     3601 cgtatcggcg accttgttgc aggtacgatc ctgatcgaca cgcgcaacca gacctcctgg
     3661 caggacacgg tctttaccga actgtcggac gactataaac cgcgctatcc gcaggtgatg
     3721 cagctgagcg ataaggatat caataccctc aagacgatca tcaattcggt gaacaggagc
     3781 ggcgactacg atctttccat gcggatcgcc gatcgcatca ggagcaagct cgatatctac
     3841 agcgatcagg attccctcga tttcctccag accctgttaa aggattacaa ctattatgct
     3901 acgcgttgac cctgccggct gtggagatga tgaggatcag gatgaacaac acccagaagc
     3961 ccaggcagat gatcatgaga acgccgacaa agcggtacag ggcccgcata ttcttgaagg
     4021 cgatgttcag ctgatcctgg tcgttgttac gcagggcgac cctcatccgg gtggcaaaat
     4081 tataaaggta aagacagggg aagaaatt
//
LOCUS       DATGFG010000260         9004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_151553, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000260 DATGFG010000000
VERSION     DATGFG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151553"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VKQ52_04845"
     CDS             complement(<1..898)
                     /locus_tag="VKQ52_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLZ86538.1"
                     /translation="MDRKEALEFLELPEYATPDEIKAKIETKLAYFENLSEKAPSPFV
                     RKLHARNVARVKEIMALSKDWPAAKPPVYTEQVAVPERIRDAAFEAPASPVKAQPAPA
                     AAPPPPTAPAVAEAPISAPPPPPPSMPPPPSAVPPMPPRKPEPEYRATAAEQESRTAA
                     TTRPFAPAKPSPAPDEPLAWLVRHTENQSPLAFPVFAGKNYIGRKEKPGATPFIKVED
                     DPYISKIHAVIIAEAGSPNAFFLADDPATNGDRASTNGSYLNGNEDRITKKTAVKNND
                     TIQVGVTKLILRVNDTTLDRIVK"
     gene            complement(903..2351)
                     /locus_tag="VKQ52_04850"
     CDS             complement(903..2351)
                     /locus_tag="VKQ52_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86539.1"
                     /translation="MRGPWIIFFMLLQGAATPLTTYAQHTFDDLPVNDPSHHSVLGPH
                     EDPAKKIRNNLFVVASFDKSICFPGQQIMLTYRLYTALQSTSAIIAKPLLNGFTAKEQ
                     KVDETPLPDKTIDGRSYHGFTIWKVLLTPLQTGSYTIDPLAVNNDVRYTAADGKPASY
                     SGTVTSNKPTINVLPLPPGGQPPAFSGLIGKWQIHSRLVSRSLDAGESDTLLIETTGT
                     GSFDNLPVPLIRWPSGFRHLDPIQRWDINENAFPQTGKKTVAIPFTPTTPGRYTLPSM
                     DLAYFDPAAGKYNVAHTDSLTLLVTGSAVTASASPSTSASPTSPSSKAPNSTSPGATP
                     PAATAPYPGRPLLEKLIFPIVVAIAVILLLVLRSRRWRPAPVPAAGPPSDPASRPSPP
                     PLPVKKLPEEELYLANLKQSLITFLQSRVKSDAWAEEELVRLLQEKDNTLAGKIRPVL
                     DLCNRLLYSPHRSDPDALGELSARVTAVLNTN"
     gene            complement(2358..2861)
                     /gene="tssO"
                     /locus_tag="VKQ52_04855"
     CDS             complement(2358..2861)
                     /gene="tssO"
                     /locus_tag="VKQ52_04855"
                     /inference="COORDINATES: protein motif:HMM:NF036668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system TssO"
                     /protein_id="HLZ86540.1"
                     /translation="MQTILNSKDRNQAFLKFLVLFLVTVTLCVLAVYFDYRLPVRENR
                     VLQEEVALQRQQDVDQQKFAGKMQEALVLMDSVDKAGVNTEQIEIQFNGKLNDLSVLQ
                     LKDNTAYGLVDKAIIYRLSELWQAKKNQASLTEKAQHAEEAQNQVAQLQQQINTLNQT
                     LATYQHK"
     gene            complement(2882..4057)
                     /locus_tag="VKQ52_04860"
     CDS             complement(2882..4057)
                     /locus_tag="VKQ52_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86541.1"
                     /translation="MQENNYLPVNWIDGMKINRSHFIAQDNAVIFHLAQVAGSMLNDY
                     NYGLLPAGNGSPALKLFLSTDNQQRVQVRIQQCRAITAGGYYFDFREDTALSGASLQA
                     PLLTNPIAFKELKGRSALFYIVLTVNPYKRIPYGAADPSELPPRNPFVRPFFSADLVP
                     VEEMTRNKPGAFQLAVGKVRIEETRALLEEDYIPPCHSISSHSDLLEIHAALEQFFGK
                     MEVWSIQILQKIFQKKQANDLSAIVQKMCEQFTVFTASRLAELKTTGIVQPPVWLVSN
                     AASMARLIKNILDAYQGSGKEELINYFTEWCNISQGELEGAIVALTNYQYDHHDINSA
                     VEKITTFTKIISQLFNQLSRLEYIGKRKDTGIFVKEEVVAAQPVEQPAPKRRSFLAD"
     gene            complement(4067..4945)
                     /locus_tag="VKQ52_04865"
     CDS             complement(4067..4945)
                     /locus_tag="VKQ52_04865"
                     /inference="COORDINATES: protein motif:HMM:NF036941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssN family type VI secretion system protein"
                     /protein_id="HLZ86542.1"
                     /translation="MERVITSELTKLLILLAAGVAGLAALTVNLISKAKGSFKPYQKA
                     TLLYLVAGILFFSIIALAAWPPMAGQPFTMLIVFQAWFLLLGILHFYTMGQYLGWAGN
                     KKSFLAETVFTVLVGLFGTIGFLLVYRFLSRDGLHYVMAGSILFFLLPFLLLHTFRRA
                     VAIPPKIVREWFYPVNEYIDEPEDSKMRNLRVISFEFQKRTSDPHFTNFRAKAPADMD
                     FGDLFYYFINDYNERHSSSRIEIADQLGSPHGWIFYKKSKWFSIATNYIDAEKTVMNN
                     KIKENDVIICTRSVNS"
     gene            complement(4980..5921)
                     /locus_tag="VKQ52_04870"
     CDS             complement(4980..5921)
                     /locus_tag="VKQ52_04870"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HLZ86543.1"
                     /translation="MDNYTGLAALREELRRVGCDARTEVCFALAMQEGLSIEDFLVGF
                     DKQFYREYSKDIMFTEIKEDAAKKPLLQVHLTRGGIFDLLPEGLFFQPPQSSRQSSAA
                     DLAAEYQVNRKKEDAIRRFFLPLENEFFWSRIQVEQEESRLLEGLQTGMLNDYFARFW
                     GLPASIPRECVAPLLILIPHAYKIAGNLALTAQCLQQLLQEEVQVRQVPPESSPLPQG
                     QEMGLGESSLGIDMTCGNEFFEDYPVMEWIIGPLVGSRVQDYLEGGRRHSLLETFTRF
                     FIPAGIDTKLTLLLPEERQHMALRPGQEPLLGFSSIL"
     gene            complement(5951..7822)
                     /locus_tag="VKQ52_04875"
     CDS             complement(5951..7822)
                     /locus_tag="VKQ52_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86544.1"
                     /translation="MQETKDHIKTRLLKNAARAWGLPETEAEANFDPLVSMLFSACAA
                     ELEKISGEIQNSRARILERMVQLLSPDSLTGALPAHAIASTTPIERHMELPENTQFFL
                     SRRQQGQSEMEDTAPKDIFFSPTAPFPLTRAAVRYMAVGNTLYQVKNTINKEVIAIAE
                     PDQELPASSLWLGIDEPGTPLGDTLFYFDIRNDAARQLFYNQLPKATWYWSDQPMAHI
                     PGYGNRNISGEQIDLETTLNRDDDTSGKIAKRVNALYKPSFITLVDPEGLSAGEDNTL
                     LASMIEEVFPGKVLKQVQKTPLRWICIDFPQAVSSRVLQDVLCVMNCFPVVNRRLHEF
                     TFRVQDIINIVPLQSEDTFLDLAEAGDDEGHLLNIRSFDSYSDDSFALLLRNGGVGRF
                     DGRDAGAIVDYLLQLLRDESAAFSTLGSDFVNGELKQLRQIINKLEQRLYSRQQQRDE
                     TPYLVIRNNNKNPWQNIFIRHWSTCGTGGNNIRSGSVMRLYKGGGLQNSQLTLVTTTV
                     GGRDRLSVTDRVMAYKSALLSRDRIITSEDIKVFCQYQLGQCVRKIDVKKGVQSFPDQ
                     QQGFTRTIDVFVDIEPRVYDDMLQRGEIAFWKDNLKLLLEERSAGLTPFRVFLGEAS"
     gene            complement(7849..8277)
                     /locus_tag="VKQ52_04880"
     CDS             complement(7849..8277)
                     /locus_tag="VKQ52_04880"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HLZ86545.1"
                     /translation="MQLEYYSLPLAVEKVMNKGEHPKCSMQQSVIQHLHLLLTTAFGG
                     FPADEGFGCGIWDNDFDNVTSAHKLREYIRETLHGSIETQEPRLGHVRVEVFLRQEEM
                     PGYSVRNIKKKIDVTITGLLSLTNEKLSYRDSFFVGPLSY"
     gene            complement(8472..>9004)
                     /locus_tag="VKQ52_04885"
     CDS             complement(8472..>9004)
                     /locus_tag="VKQ52_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86546.1"
                     /translation="NSGDQKVTVVLLPDRTGVVGIGSAQTGGPPPTYSIGEYEYAGGK
                     ITRLPTFGYTFTIFTTYKIGVNIQNNSAYGRGTVEDDKNTSLQFHESQHGFFIVKYLV
                     DHPLPKAAIHLGMSVHEYKTALKAFDVQFKKYTATMDKQNKINVDCVGTLPKKGYSKE
                     LDSLCYKRIPNKAKRR"
BASE COUNT         1727 a         2126 c         2950 g         2201 t
ORIGIN      
        1 cttttacgat gcgatcgagc gtggtatcat tgacgcgcag gatgagtttt gtgacgccga
       61 cctggatggt gtcgttgttc tttaccgcgg tcttttttgt gatacggtct tcgttcccat
      121 tcaaatagct gccgtttgta ctggcccggt cgccgttggt ggcggggtcg tccgcgagga
      181 agaaggcgtt ggggctgccg gcttcggcga tgatgacagc atgaatttta ctgatgtagg
      241 ggtcgtcctc cacttttatg aagggggtgg cgccgggttt ttccttgcgg ccgatgtaat
      301 tcttgccggc gaagaccggg aaggcgagcg gggattggtt ctccgtgtgg cggaccagcc
      361 aggcgagggg ttcgtcgggt gcgggtgacg gttttgccgg tgcgaaaggg cgggtggtgg
      421 cggcagtcct ggattcctgt tcggcggcag tggcgcgata ttccggttcg ggtttcctgg
      481 gcggcatggg gggaacggcc gaaggtggtg gcggcatcga tgggggcggt ggaggtggcg
      541 cggagatggg ggcttcggcg acagcaggtg ccgttggcgg gggcggcgcg gcggcgggag
      601 cgggctgtgc cttgacggga gaggccgggg cttcgaacgc cgcatcgcgg atcctttcgg
      661 ggacagctac ctgctccgta tagacgggcg gctttgcggc gggccagtct tttgacaggg
      721 ccatgatctc cttcacgcgg gcgacattgc gggcatgcag tttccggacg aagggacttg
      781 gcgctttttc gctcaggttt tcgaaatagg ccagtttcgt ttcgatcttt gcctttatct
      841 catcgggcgt tgcatattcg ggcagttcca gaaattcgag ggcttcttta cgatccatat
      901 cgttagttgg tgtttaatac cgccgtcaca cgggcgctga gttcgcccag cgcgtcggga
      961 tcggaacggt gtggtgaata taataaccgg ttgcagagat cgagcacggg tctgatcttt
     1021 ccggcgagcg tattatcttt ttcctgcaag agtcgcacca attcctcctc ggcccaggca
     1081 tcgcttttca cccgcgactg gagaaaggtg atgagggatt gtttgaggtt ggcgagatag
     1141 agctcctctt cgggaagttt ttttactggc agcgggggcg gggacggacg ggacgcaggg
     1201 tccgatggtg ggccggccgc cgggaccggc gcgggacgcc agcgtctcga gcggagaacg
     1261 agcaggagga tgaccgcaat ggccacgacg atcgggaaga tgagtttttc gaggagcggc
     1321 cttcccgggt atggggccgt ggccgcgggt ggagtggcgc cgggcgacgt ggagttgggc
     1381 gccttggagc tgggcgacgt gggagacgcg gatgttgagg gggaggcgga tgctgtgact
     1441 gccgatccgg tgacgaggag ggtcaggctg tcggtatgcg cgacattgta tttccccgcg
     1501 gcgggatcga agtaagcgag gtccatcgaa ggcagggtgt accggcccgg ggtggtcgga
     1561 gtgaacggga tggccaccgt cttttttccc gtctggggga aggcattctc gttgatatcc
     1621 cagcgttgaa tgggatcgag gtggcgaaag ccggaaggcc agcggataag cggcaccggg
     1681 agattatcga agcttcccgt tcccgtcgtt tcgatgagca aagtgtcgct ctcccccgcg
     1741 tcgaggctgc gggatacgag ccggctatgg atctgccatt tgccgatgag gccggaaaaa
     1801 gccgggggct gtccgcccgg aggcaggggc aggacattga tcgtgggctt attgctggtg
     1861 acggtgccgg aataggaagc gggtttaccg tcagcggcag tatagcggac atcgttgttg
     1921 accgcaagcg ggtcgatggt atagctaccg gtctgcaagg gggtgagcag caccttccag
     1981 atggtgaagc catggtagct gcggccgtcg atggtcttgt cgggaagcgg cgtctcgtcc
     2041 accttctgct cctttgccgt aaagccgttc aacagaggtt tggcgatgat ggccgaggtg
     2101 ctttgcaggg cggtgtagag acgataggtg agcatgatct gctggccggg gaagcagatg
     2161 gacttgtcga aggaggcgac gacgaagaga ttgttcctga tctttttggc ggggtcttcg
     2221 tgcggcccga gcaccgaatg gtgggacgga tcgttgacgg ggaggtcgtc aaaggtatgc
     2281 tgggcatagg ttgtcagggg cgttgcggcc ccctgcaaaa gcataaaaaa gatgatccat
     2341 ggcccacgca tgaaaattta tttgtgttga taagtggcca gggtctggtt cagcgtgttg
     2401 atctgctgtt gcagctgggc cacctggttt tgcgcttctt cggcgtgctg ggccttttcc
     2461 gtgagcgagg cctggttctt ctttgcctgc cagagttcac tcaaccggta aatgatagct
     2521 ttatcaacga ggccgtaggc cgtgttgtcc tttagctgga ggacggaaag gtcgttcagt
     2581 tttccgttga actggatctc gatctgttcg gtattgacac cggccttatc cacggagtcc
     2641 atcagcacca gggcttcctg cattttgccg gcgaatttct gctggtcgac gtcttgttgc
     2701 cgctggagcg ccacttcttc ctggaggacc ctgttctcgc gaacgggaag acggtagtcg
     2761 aagtagacgg ccagtacaca gagggtaacg gtcacgagga agaggaccag gaatttcagg
     2821 aaggcctggt ttcggtcttt gctattcaaa atggtttgca tggtagagtt ttatgtttgg
     2881 gttagtcggc gaggaagctc ctgcgttttg gcgcgggttg ttcgacgggc tgggcggcga
     2941 cgacctcctc cttgacaaaa atgccggtgt ccttgcgttt gccgatatat tcgagtcgtg
     3001 acaattggtt gaagagttgg gaaattattt ttgtgaaggt agtgatcttc tccaccgcgc
     3061 tgttgatatc gtggtggtcg tattggtaat tggtgagcgc cacgatggcg ccttcgagct
     3121 caccctggct gatattgcac cattcggtga aatagttgat cagttcctct ttgccgctgc
     3181 cctggtaggc gtcgaggata ttctttatga ggcgggccat ggaggcggcg ttgctgacca
     3241 gccagacggg tggctgaacg ataccggtcg tttttagttc ggccagccgg gatgccgtga
     3301 agaccgtgaa ttgttcgcac atcttctgga cgatggcgga caggtcgttg gcctgtttct
     3361 tctggaagat cttctggagg atctggatgg accagacctc catcttgcca aagaattgtt
     3421 cgagggcggc atggatctcg aggagatcgg aatggctgga gatggagtga cagggcggga
     3481 tatagtcttc ttccagcagg gcccttgtct cttcgatccg taccttgccg accgccagtt
     3541 ggaaggcgcc gggtttgttg cgggtcatct cttccacggg gacgaggtcg gcggagaaga
     3601 agggacgcac gaaggggttg cgtggcggaa gttcggacgg gtcggcggcg ccgtagggga
     3661 tgcgtttgta tgggttcacc gtcagcacga tatagaagag ggcggagcgt cccttcagct
     3721 ctttgaaggc gatgggattg gtcagcaggg gcgcctggag ggaagcgccg ctgagggccg
     3781 tgtcctccct gaagtcgaaa tagtatcccc ctgccgtgat ggcgcggcac tgctggatgc
     3841 gcacctggac gcgttgctgg ttgtcggtgc tgaggaataa ttttaaggcg gggctgccat
     3901 tgccggcggg caacaggcca tagttataat cgttcagcat gctcccggcc acctgcgcga
     3961 ggtggaagat cactgcattg tcctgcgcga tgaaatgact ccggttgatc ttcattccat
     4021 cgatccagtt taccggtaaa taattgtttt cctgcatacc cgtagtttat gaatttacag
     4081 atcttgtaca aatgatcacg tcgttctctt ttatcttatt gttcatgacc gtcttttcgg
     4141 cgtcgatata gttggtcgcg atggagaacc atttcgattt tttatagaat atccagccgt
     4201 ggggtgatcc gagctggtcg gcgatctcga tgcggctgct ggaatggcgt tcgttataat
     4261 cgtttatgaa atagtagaac agatcgccga aatccatatc ggccggagct tttgcgcgga
     4321 agttcgtgaa gtggggatcg cttgtccttt tctggaattc gaagctgatg acgcggagat
     4381 tgcgcatctt gctgtcttcc ggctcatcga tatattcgtt gacggggtag aaccattccc
     4441 ttacgatctt cggtgggatg gcgacggccc tgcggaaagt gtggagcagg aggaagggca
     4501 gcaggaagaa gaggatgctt ccggccatca cataatgcag gccatcacgg ctgaggaaac
     4561 gataaacgag caggaagccg atggtcccga acagacccac caggacggta aataccgttt
     4621 ctgcgaggaa ggatttcttg ttccctgccc agccgagata ctggcccatg gtgtagaaat
     4681 gcaggatgcc gagcagcagg aaccaggcct ggaagacgat cagcatggta aaaggctgac
     4741 cggccatcgg cggccaggcg gccagggcga tgatcgaaaa gaaaaggatg cccgcgacaa
     4801 gatagagcag ggtcgctttc tgatagggtt tgaaactacc ctttgctttt gagatgagat
     4861 tgacggtcaa ggcagccagt ccggccaccc ctgcggcgag caggatcagg agtttggtca
     4921 attcacttgt aatcactcgt tccatgttac cgattcagct atttttcgtt attcgttttt
     4981 tataatatgc tggaaaatcc cagcaggggc tcctgcccag ggcgcaaggc catatgttgt
     5041 cgttcctccg gcaggaggag ggtcagtttt gtgtctatgc ccgcgggtat gaagaaacgg
     5101 gtaaaggttt ctagcaggct atgccgccgg ccgccctcga gataatcctg gacacgggag
     5161 ccgacgaggg gaccgatgat ccattccatc acggggtagt cttcgaagaa ttcgttaccg
     5221 caggtcatgt cgatacccag gctgctttcg ccaagcccca tctcctggcc ctgtggaagg
     5281 ggagagcttt ccgggggcac ctgtcttacc tgcacctcct cctgcagcaa ttgctggagg
     5341 cactgggcgg tgagagcgag gttaccggct atcttgtatg catgggggat caggatgaga
     5401 aggggggcga cgcattcgcg gggaatggag gcgggtagtc cccagaaccg ggcgaagtag
     5461 tcattcagca tgcccgtctg caggccttcc agcaggcggc tttcttcctg ttcgacctgg
     5521 atgcgcgacc agaagaattc gttttccagc ggcaggaaga aacggcggat ggcgtcttct
     5581 tttttcctgt ttacctggta ttcggccgcc aggtcggcgg cggaggattg ccgggaggat
     5641 tgcggcggct ggaagaagag gccttccggc aggaggtcga agatgccgcc gcgggtgagg
     5701 tggacctgca ggagcggctt ttttgcggcg tcctccttga tctcggtgaa catgatatcc
     5761 ttgctgtatt cgcggtagaa ctgtttgtcg aagccgacca ggaagtcttc gatggacaat
     5821 ccttcctgca tggcgagcgc aaagcagacc tccgtcctcg cgtcgcagcc gacgcgccgg
     5881 agttcttccc gcaatgctgc tagccctgta tagttgtcca tcatcggtgg tttatgcttc
     5941 ttgtcctttt ttatgacgct tccccgagaa agacgcggaa gggggtgagt cccgccgatc
     6001 tttcttcgag cagtaatttc aggttgtctt tccagaaggc gatctccccg cgctggagca
     6061 tatcgtcgta tacccttggt tcgatgtcga caaagacgtc gatggtccgg gtaaagccct
     6121 gttgctgatc ggggaagctt tgcacgcctt tctttacgtc tatcttccgt acgcactggc
     6181 ccaactggta ttggcagaat acttttatgt cttcgctggt gatgatccgg tcacgggaca
     6241 gcagggcgct tttgtaggcc atgacccgat cggtaacgct gaggcggtcg cgaccgccga
     6301 cggttgtcgt gaccagggtg agctggctgt tctgcaggcc gccgcctttg tagagccgca
     6361 tgacgctgcc ggagcggata ttatttcctc ccgtcccgca ggtgctccag tggcggatga
     6421 agatattctg ccaggggttc ttattgttgt tgcggatgac gagatagggg gtttcatcgc
     6481 gttgttgctg gcgggagtaa agccgttgtt ccagcttatt gatgatctgg cgcagctgtt
     6541 tcaattcacc gttgacaaaa tcgctgccga gggtggaaaa ggcggcgctt tcgtcgcgga
     6601 gcaactggag aagatagtcc acgatagcgc ccgcgtcgcg accgtcgaag cgccctaccc
     6661 caccgttgcg caacagaagc gcgaacgagt catcgctata gctgtcgaag gagcggatat
     6721 tcagcaggtg gccttcgtca tcgccggctt cggcgagatc gaggaaggtg tcttccgatt
     6781 gcaggggaac gatattgatg atatcctgta cgcggaaggt gaattcgtgg aggcggcggt
     6841 tgaccacggg gaagcagttc atcacgcaga gtacgtcctg cagcacacgg ctgctgacgg
     6901 cctgggggaa gtcgatgcag atccagcgaa ggggtgtctt ttgcacctgt ttcaggactt
     6961 tgccggggaa gacctcttcg atcatggaag cgaggagggt attgtcctcg cctgctgaaa
     7021 gcccttcggg gtccaccagg gtgataaagg agggcttata gagggcgttc acccgtttgg
     7081 ctattttgcc gctggtgtcg tcgtcgcggt tgagggttgt ctcgaggtcg atctgttcgc
     7141 cgctgatgtt gcggttgcca tagccgggaa tatgggccat gggctggtca ctccagtacc
     7201 aggtggcttt tggcagctgg ttgtagaaga gttgccgggc cgcatcgttg cggatatcaa
     7261 aatagaaaag ggtgtcgccg agcggggttc ccggttcgtc gatgcccagc cataaggaag
     7321 aagcgggcag ttcctgatcc ggctcggcga tggcgatcac ctctttgttg atggtattct
     7381 tcacctggta gagggtgtta cccacggcca tatagcgaac ggcggcgcgg gtaaggggga
     7441 agggggccgt cggcgaaaag aagatatctt ttggagcagt gtcttccatt tccgattggc
     7501 cctgttgccg gcgggaaaga aagaactggg tgttctccgg cagctccatg tgtcgttcga
     7561 tgggtgtcgt ggaggcgatg gcgtgggcgg ggagggcgcc cgtgaggctg tcgggggaca
     7621 gcagctggac catgcgttcg aggatgcggg cgcggctgtt ctggatctcg ccggagatct
     7681 tttcgagttc ggccgcgcag gcggagaaga gcatcgatac caggggatcg aagtttgctt
     7741 cggcctccgt ttcggggagt ccccaggcgc gggcggcgtt cttcagcaat cgcgttttta
     7801 tgtggtcctt tgtttcctgc atggtgtcgt tcgttggtta ggataggatc agtaggataa
     7861 ggggccgacg aagaagctgt cccggtagga cagtttttcg tttgtcaggg acaaaaggcc
     7921 ggtgatggtg acgtcgatct tcttttttat gttgcggacg gagtagccgg gcatttcttc
     7981 ctggcggagg aagacctcca cgcggacatg cccaaggcgc ggctcctgtg tttctatcga
     8041 gccgtggagg gtctcgcgga tatattcccg gagtttgtgg gcgctggtga cgttgtcgaa
     8101 atcgttgtcc cagatgccgc agccaaagcc ctcgtcggcg ggaaagccgc caaacgctgt
     8161 cgtgagcagc aggtggaggt gttggataac cgattgctgc atcgagcatt tgggatgttc
     8221 gcccttgttc atcacttttt ccaccgccag tggcagggaa tagtactcca attgcatatg
     8281 actataatta gacttcgtag atacttggaa acaaattagt taagggccga cgggtaattt
     8341 tcgtccacaa ggctcaaaag ggtttgtcct gagctgctcg cggtgggtca aaagtgctga
     8401 ttctcaagga ttatggaggg aactaaatgt acgataaagt tatgggatat gcaaggggag
     8461 aaagacggaa attatcgtct tttcgccttg ttgggaatcc gcttgtagca gaggctgtcc
     8521 aactctttac tatacccttt tttgggcagt gtgcctacac aatcgacgtt aatcttattt
     8581 tgtttatcca ttgttgccgt atacttttta aattgaacat caaaagcttt gagagctgtc
     8641 ttatactcgt gcaccgacat ccctaaatgg atcgctgctt tcggcaacgg atgatccacc
     8701 agatatttca caataaagaa tccatgttga gattcatgga attgcaaaga agtattcttg
     8761 tcgtcctcca cagtaccgcg cccataagcg gagttatttt gaatattaac accgatctta
     8821 tatgtggtaa atatggtaaa tgtatagcca aaggttggaa gccgggttat tttgcctccc
     8881 gcatattcat attctccaat ggaatatgta ggtggggggc cgccggtttg ggcacttcca
     8941 attcctacga cgcctgtccg atcgggtaat agcaccacgg ttactttttg atcaccggaa
     9001 ttga
//
LOCUS       DATGFG010000261         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1548650, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000261 DATGFG010000000
VERSION     DATGFG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548650"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VKQ52_04890"
     CDS             <1..1016
                     /locus_tag="VKQ52_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86547.1"
                     /translation="ENFGFWEYGYRIDTAENRDGQPKLLTGELYGKHFCIIADSMRKA
                     AAETGASIYIGAVTVEASHPWDLPIARNWNEGMMKAGHNSPDFYVVHNYFTPYNKNSS
                     AAEILYSAATTPGEQMRFVTKCLHDNGAAIKPIAMDEWNMFATGNGQQVSNTSGLFAV
                     LVMGEALRNKYGMAARWDLYNGWDNGNDHGLFSAGDEPGVARWTPRPSFYYLYYLQRM
                     LGDRLLGSTLTGDTSLRAYASSFSSGEIGATVVNPSPNAATIRLDIRHFKPGKQFCWY
                     TLSGEGDGEFPRKVTVNGQTTTAAAGGPPNYATLPAYTAPTAKGISVTIPARGAVFLV
                     VEK"
     gene            1259..1852
                     /locus_tag="VKQ52_04895"
     CDS             1259..1852
                     /locus_tag="VKQ52_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86548.1"
                     /translation="MPPTRKDLENPMGGLFLMMIFTTIWAIIAEVALELRDYGLLGGF
                     FALMLLYLLAHYIKFHRVQQLLPKAPENAETAEDRKRSRQFLYIFGTEGLAILVVKNI
                     LVNVNLDHLFIPCLALIVGLHFFPLAKIFRRKFDYYVAAWTTLIGLAGLTMMVERMTP
                     PNTAIAIVAIGCALATAANGVRAVINGNRIIGRSYPE"
     gene            2182..2424
                     /locus_tag="VKQ52_04900"
     CDS             2182..2424
                     /locus_tag="VKQ52_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86549.1"
                     /translation="MLNEGQIQSFLDIFQYIPKSVVAADLGKKVDRFTELMNRIEKFT
                     VEELIIIAGFCNLSISEMFRLVETEYLKRQNKKLKV"
BASE COUNT          728 a          784 c          685 g          618 t
ORIGIN      
        1 acgagaattt cggcttctgg gaatatggtt accggatcga cacggcggaa aacagggatg
       61 ggcaaccgaa attgctcacc ggcgaactct atggaaagca tttctgcatc atcgcggact
      121 cgatgcggaa agccgccgcg gagacgggag catcgatcta tatcggcgcc gtcaccgtgg
      181 aagcctcaca cccctgggac ctccccatcg cccgcaactg gaacgaaggc atgatgaagg
      241 cagggcataa cagccccgac ttctatgtcg tacacaacta tttcactccg tataataaga
      301 acagcagcgc cgccgagatc ctctacagcg ccgcgacgac ccccggcgaa cagatgcgct
      361 tcgttacgaa atgcctccac gacaatggcg ccgcgataaa acccatcgcc atggacgaat
      421 ggaatatgtt cgccaccggc aacgggcagc aggtatccaa caccagcggg cttttcgccg
      481 tcctggtcat gggggaggca ttacgcaata aatatgggat ggcagcgcgc tgggatctgt
      541 acaatggatg ggataacggc aacgatcatg gcctctttag cgcgggcgac gagccgggag
      601 tggcccgatg gacgccgcgc ccttcgttct actatctcta ttatctccag cggatgcttg
      661 gcgatcgcct gctgggcagc accctcaccg gcgacaccag cctgcgggcc tatgcttcct
      721 ccttctcctc cggcgagatc ggcgcgacgg tcgtcaatcc atcgcccaat gccgccacga
      781 tccggctcga catccgccat ttcaaacccg gtaaacagtt ctgctggtac accctctccg
      841 gcgagggcga cggcgagttc ccgcgaaagg tcaccgtcaa cggccaaacc actacggctg
      901 ccgccggcgg cccccccaac tacgccacac tgccggccta cacggcgcca accgcaaaag
      961 ggatcagcgt cacgatcccc gcccgcggtg cggtgttcct cgtggtggag aaatgatccg
     1021 ggaaaaattg ccggcgcttc cgacactatc gccaacgaca ggttgcagcc ggcaaacagg
     1081 cagtcgacga atctccattc ggcgagattc acggaagaca gatcgcagtg tagaaaacta
     1141 caatggtcat aaactccctt caccggcggc tctgttttaa aatccgtttt atcgaaggtt
     1201 tgatcttcaa caaaagcatc cataaccgta agataacgta tatttaagca aaaacgcgat
     1261 gcccccaacg agaaaagacc tggagaaccc gatgggcgga ttgttcctga tgatgatctt
     1321 caccaccatc tgggcaatca tcgccgaggt ggcgctggag ctgagggact atggcctgct
     1381 aggaggattt tttgcgttga tgttgctcta tcttctcgcc cattatataa aattccatcg
     1441 cgtgcaacaa ctgctgccaa aagcgccgga gaatgccgag accgcggagg accgtaaacg
     1501 gtcccggcag ttcctctaca tctttgggac ggaaggtctc gcgatcctcg tcgtcaaaaa
     1561 tatcctcgtc aatgtcaatc tcgaccatct ctttatcccc tgccttgcac tgatcgtcgg
     1621 attgcatttc tttcccctgg caaagatctt ccgccggaaa ttcgactatt acgtggccgc
     1681 atggacgacg ctgatcgggc tggcgggact aacgatgatg gtggaaagga tgacgccgcc
     1741 caatacggca atcgccatcg tcgcaattgg ctgtgcactc gccactgccg ccaacggggt
     1801 ccgggccgtg atcaacggca accgcattat cggcagaagc tatcccgaat aaaaattatt
     1861 ttctcccctg aatacaccgg taaatccatg ataaacaggt gggaaaacga caatccaagg
     1921 ggtcgatccc gtcagataat actaaaaaaa ttagtatttc gatcgactat tcagtatctt
     1981 tatatgggct ttttcggagt acggccggtg ccggtcataa ccatgaaaaa ccgggatata
     2041 aaaggagaat atccgggata aaaaatagga ataatcgggg tactcaatag gaaaaagcgg
     2101 gatactcatt acctggaata cttataaatc ctttctttga ctatgcggac gcgggactct
     2161 cgttatcgga tcattaaacc tttgctgaat gaaggccaaa tacaatcctt cctggatatt
     2221 ttccaatata tccccaaatc ggtagtagcc gccgatctgg gaaagaaggt agaccggttc
     2281 accgaactga tgaaccggat cgagaaattt acggtagaag agctgatcat catcgccggc
     2341 ttttgcaacc tgagcatctc cgagatgttc aggttagtgg agaccgaata tctcaagcga
     2401 cagaacaaaa aattgaaagt ctgaccttgt ccttattgcc atagccattg cctgagggcg
     2461 cctttcctat tacccacccg gattaaacac ctcatcgcgc accgtctcaa atgagacggt
     2521 tttttttata cggagtcacg ggaaaccact tattttattg acaattcttc gcatgtaaac
     2581 gattgtaatt gaacaaaatc gccagtttta gcacactaag tgtttttctt acacgatgag
     2641 aacatcccaa tttgtcaccg ttacgtaact tttacagaga aacaggatcc cggacgcctc
     2701 tatcctttga cccgtttaac gagcaatttc taccgctatg atcccttcgc aaaaagatat
     2761 tgacggcgcc tatgccgtaa agatctacct ggataaccat ccctttcgcc aggta
//
LOCUS       DATGFG010000262         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_605207, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000262 DATGFG010000000
VERSION     DATGFG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605207"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VKQ52_04905"
     CDS             <1..261
                     /locus_tag="VKQ52_04905"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86550.1"
                     /translation="GPGNAQPLFVARRLTDRGSRIVKEQHIRFCLQQGNLQADGIGFG
                     LAQKFALLQAGTPVDVVFTLEENEWNNIKTLQLKVIDVAPSL"
     gene            complement(268..2118)
                     /gene="htpG"
                     /locus_tag="VKQ52_04910"
     CDS             complement(268..2118)
                     /gene="htpG"
                     /locus_tag="VKQ52_04910"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HLZ86551.1"
                     /translation="MQKGNIRVQTENIFPIIKKFLYSDHDIFIRELVSNAVDATQKLK
                     TLSSLGEAKGDLGDLSIEIQLDIEKKTITISDKGVGMTGEEVDKYLNQVAFSGAEEFL
                     KKYKGQNEANIIGHFGLGFYSSFMVSERVDVITKSYQENAQAVKWECDGSPEYTLENT
                     EKADRGTDIVLHINEESKEFLDAERIRTILKKFCRFLPIPIRFEGQQINNPTPAWTRK
                     PSELTSQDYQDFYKELYPYGEAPLFWIHLNVDYPFNLTGILYFPKIRQSYEIQKDKIQ
                     LYSNQVFVTDEVSDIVPEFLMLLHGVLDSPDIPLNVSRSYLQGDPNVKKINSHITKKV
                     ADKLEEIFTKERKEFEEKWESLGLFIKYGMMTDDKFLEKATKFHIMEDAKGGSFYTLE
                     EYRTAAEGLQKNKDGQLVILYTTNPVQQDSYIQQAQAKGYIVVKLETIVDAAFINQME
                     TKWEKVHFARVDADIVDNLIDKAETSGSVLSGDEESVLKELFGRQMPELHMTVEVKGL
                     SPEAPPVVATRPEFMRRMKDMAAMNGAMGGFYASMPDEVTLTVNGNHPIYQTILKEGD
                     RSRQEKLVHNLSDLALLSQGLLKGNSLTHFIQRSVDLMGGEAKSKIILEA"
     gene            2299..2652
                     /gene="rpsF"
                     /locus_tag="VKQ52_04915"
     CDS             2299..2652
                     /gene="rpsF"
                     /locus_tag="VKQ52_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HLZ86552.1"
                     /translation="MNNYELMVIFTPVLSEEDYKAAQKKYASLVKDNGGEVSHENPWG
                     LKSLAYPIQKKTTGLYWVLEYSAPSDFNEKLKIQILRDETVLRFMATKLDKYAVEYNA
                     KKRSGVPTGTEKVGA"
     gene            2656..>2873
                     /gene="rpsR"
                     /locus_tag="VKQ52_04920"
     CDS             2656..>2873
                     /gene="rpsR"
                     /locus_tag="VKQ52_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HLZ86553.1"
                     /translation="MAKANEIKYLTAIKPEKRAKKFCRFKKYGIKYIDYKDAEFLKKF
                     LNEQGKLLPRRITGNSLKYQRKVSDAVK"
BASE COUNT          688 a          721 c          690 g          774 t
ORIGIN      
        1 ggacccggca atgcccaacc gctatttgtg gcgcgccgcc tgacggacag gggatcacgt
       61 atcgtgaagg agcaacatat ccgcttctgt cttcagcagg gaaacctcca ggcggacggc
      121 atcggtttcg gcctggccca aaaattcgct ctcctccagg cgggaacacc agtggatgta
      181 gtattcacac tggaagagaa cgaatggaat aatataaaga ccttacaatt gaaagtgatc
      241 gacgttgcgc cttcgctcta gtttgcgtta ggcttccagg atgatctttg actttgcttc
      301 accccccatc agatccacgc tgcgctggat aaaatgggtc aggctattgc ctttcagcaa
      361 gccctgggac aggagcgcaa ggtcggagag gttatggacc agcttttcct gcctggatct
      421 gtccccctct ttaaggatag tttgataaat aggatggttg ccgttcaccg taagcgttac
      481 ttcatccggc atactcgcat aaaaaccgcc catcgccccg ttcatggcag ccatatcctt
      541 catccggcgc atgaattccg gccgggtggc aacgacgggt ggcgcctcgg gactcaatcc
      601 ttttacctct accgtcatat ggagttcggg catttgccgg ccgaacaatt cctttaagac
      661 cgattcttca tcgccgctaa gaacactgcc ggaggtttcc gccttatcga tcaggttgtc
      721 gacgatatcg gcatccacac gggcgaaatg cactttttcc catttggtct ccatctggtt
      781 gataaaagcg gcatcgacaa tggtctctaa tttgacaacg atatatcctt ttgcctgcgc
      841 ctgctggata tagctgtcct gctgcacggg gttcgtagta tagaggatga ccagttggcc
      901 gtccttgttc ttttgcaggc cttcggccgc cgtccggtat tcctccaggg tatagaagct
      961 gccgcctttt gcgtcttcca taatatggaa tttcgtggct ttctcgagga acttgtcatc
     1021 cgtcatcatc ccgtatttga tgaacagccc gaggctttcc catttctctt cgaattcctt
     1081 tctctccttt gtaaagatct cctccagttt atcggccacc ttctttgtga tgtgactatt
     1141 tatcttcttc acattcggat caccctgcag gtagctcctg cttacattga gcggaatatc
     1201 cgggctgtcg agaaccccat gcagcagcat caggaattca ggcacgatat cgcttacttc
     1261 atcggtaacg aacacctggt tgctgtacag ttggatcttg tctttctgta tctcgtagct
     1321 ctgcctgatc ttcgggaagt agaggatgcc cgtcaggttg aacggatagt ccacgttcag
     1381 gtggatccag aataaggggg cctcgccata gggatagagt tccttataga aatcctggta
     1441 gtcctgcgaa gtcagttcgc tgggttttct cgtccaggca ggcgtcggat tgttgatctg
     1501 ctgcccttcg aaccggatgg gtatgggcag gaaacggcag aattttttga ggatggtccg
     1561 gatgcgttcc gcgtcaagga attccttgct ttcctcattg atatgcaaca cgatatccgt
     1621 gccgcgatcg gccttttcgg tgttttcgag ggtatattcg gggctaccgt cacattccca
     1681 tttaacggcc tgggcatttt cctggtagct tttggtgatg acgtccaccc gttcgctgac
     1741 catgaaggac gaatagaacc caagaccgaa atgaccgata atgttggcct cattctgccc
     1801 tttatacttc ttcaggaact cctcggcgcc gctaaaggcc acctggttga gatatttatc
     1861 cacttcttcg cctgtcatgc ccaccccctt atcggaaatg gtgatggtct ttttctctat
     1921 atctaattga atttcaattg ataaatcacc cagatccccc tttgcctcgc ccagggagga
     1981 caaggttttg agcttttggg tagcgtctac cgcgttgctg accaattccc ggatgaaaat
     2041 gtcatgatcg ctgtacagga acttcttgat aataggaaaa atgttttccg tctggacccg
     2101 aatatttcct ttttgcatgg caaatagtgt tttatgaaat ggtttattag gctggctgac
     2161 caggcttagg gctcaaatca aagaaaatgc cacttgtccc tcggctgaca cagtgtcaag
     2221 tttgctattt cggttcgaaa cccgtagctt tgcagcccgt ttcatatttt ttatttaaaa
     2281 aacaactaaa cagggatcat gaacaattat gaattgatgg tgatttttac ccctgtgctt
     2341 tctgaggagg actacaaagc cgcccagaaa aaatatgctt ctctcgtaaa agacaatggc
     2401 ggagaagtat cgcacgaaaa tccctggggt ctgaaatcac tggcgtatcc catccagaag
     2461 aaaaccaccg gtttatactg ggttctggag tacagtgcgc catccgactt caatgagaag
     2521 ctgaagatcc agattctccg cgacgaaacc gtgttacgtt tcatggctac caaactcgat
     2581 aaatacgccg tcgagtacaa tgccaaaaag agaagtggtg taccgacagg aaccgaaaaa
     2641 gtgggggcat aatccatggc aaaagcaaac gagatcaagt atctgacggc gataaagccc
     2701 gagaaacgcg cgaagaaatt ctgccgtttc aagaagtacg gtatcaagta tatcgactat
     2761 aaggatgccg aattcctcaa gaaatttctg aacgagcagg gtaagttatt gccccgccgt
     2821 ataaccggca attccctgaa atatcagcgg aaagtgtccg atgcagtaaa gaa
//
LOCUS       DATGFG010000263         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_91282, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000263 DATGFG010000000
VERSION     DATGFG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91282"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VKQ52_04925"
     CDS             <1..247
                     /locus_tag="VKQ52_04925"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HLZ86554.1"
                     /translation="QRAGFSSDGIILNPGGYTHTSVAIGDAIAAIPAPVIEVHISNVH
                     AREAFRQISHVSAKAKGTIAGLGLSGYALAVRYFLPA"
     gene            complement(275..952)
                     /locus_tag="VKQ52_04930"
     CDS             complement(275..952)
                     /locus_tag="VKQ52_04930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLZ86555.1"
                     /translation="MKLYIFLFLLPVGGAGLRAQSKGATVVQPSSSVPTTIPQIKTEL
                     EKSPNPILYTRDILKKRFRIDTIIVSRTRSFTGIADSLAYHGKEKKVYGPYGPAGNRF
                     LVQLLSKAPNQFYHISQIFIDTAVFRRRVADSIGNAIMAKIKDGTATFEQMAQTYSMG
                     GEGATKGDLGWIARGVLMPTIEHEVLVHKKGEVFKIWSPAGLHIIRKTDDPKQDTGFA
                     LMLQIFL"
     gene            1033..1692
                     /locus_tag="VKQ52_04935"
     CDS             1033..1692
                     /locus_tag="VKQ52_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86556.1"
                     /translation="MRRILFFMFLAMLLTASRAYSQWKTYMISPKGDTLNCVDSKDRK
                     QGRWVNHVDEVRGEPGYEEEGMFRNDRKEGTWRLYTLQGDLSGVEFYKWGLKDGVCQY
                     FGMNGTLQREESWRALNPDKEYDTIQVEDVDHLDHYKTVIIKNEGAAIRDGVWKYYDP
                     STGMVSRQETYTLGKLEHPASQQTAASTAPKTVAKPKEVLDFEKKIGKKKVKVQDGSV
                     Y"
     gene            1838..2494
                     /locus_tag="VKQ52_04940"
     CDS             1838..2494
                     /locus_tag="VKQ52_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86557.1"
                     /translation="MMSLPKVVRGAILTGDIVNSTQLLLEEGTKLVTVLGLFLAHNPH
                     EFYRGDSFQAYIKKPDEALRLALACRCLAIGITEARREDTASIADVRISIGIGEVPTS
                     LKSLGVARGEAFLLSGRQFDSLRETGRRLAIRCNDNEPIANIGFEVMTDYLDSICRKM
                     TPTQANLIVELLRGTSQQQYANTFQKSKSTVSQLANAGRWPEIEKVLKQYEQLINLIQ
                     "
     gene            2491..>2673
                     /locus_tag="VKQ52_04945"
     CDS             2491..>2673
                     /locus_tag="VKQ52_04945"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HLZ86558.1"
                     /translation="MMPSHLWLTKLILSHLLTDFVLQPSSWVEDRGKKHFASRKLYLH
                     ALITAGLAFILIGWQYW"
BASE COUNT          624 a          606 c          801 g          642 t
ORIGIN      
        1 gcaacgggcg gggttctctt ccgacggtat tatcctcaat cctggtggat acacgcatac
       61 ttcggtggcc atcggtgatg cgatcgccgc cattcccgcg ccggtgatcg aagtgcatat
      121 cagcaatgta catgcccggg aagcgttccg gcagatctct catgtctccg ccaaggcgaa
      181 ggggacgatc gccgggctgg ggttgagcgg ctatgcactg gcggtgcgtt atttcctgcc
      241 ggcctgagcc cggttatcca ttcggtgacg cccgttataa gaagatctgc agcatcagtg
      301 cgaagcctgt gtcctgcttt ggatcatccg ttttccggat gatgtgtaag ccggcggggc
      361 tccagatctt gaagacttcc ccttttttat ggaccagcac ctcgtgctcg atggtgggca
      421 tgaggacacc gcgggcgatc cagcccaaat ctccttttgt tgccccttcc ccacccatgg
      481 agtaggtttg cgccatctgc tcgaaagttg cggtgccatc tttgatcttt gccatgatgg
      541 cgttcccgat ggagtcggcg acacgccggc ggaatacggc ggtatcgatg aagatctggc
      601 tgatatggta gaattgattg ggggccttac tcaacagctg taccaggaac cggttgccgg
      661 cggggccata gggcccatag acctttttct ctttgccgtg gtaggccagg ctgtcggcga
      721 tgccggtgaa gctgcgggtc ctgctgacga tgatggtatc gatcctgaac ctttttttca
      781 gaatatcccg ggtatagagg atggggttgg gggacttttc cagttcggtt tttatctggg
      841 ggatggtagt gggaacggag ctggagggct gaaccacggt tgcgcctttt gactgggccc
      901 ggaggccggc gccgcctacc ggcaataaaa acagaaatat gtatagtttc atgtcaatca
      961 gttggatatg tgctggaatt gattgtttgc aaatatcaat aaattggaac gattttcgtt
     1021 aattttaaag taatgcgaag aatcttattt tttatgttcc tggcgatgct gctgaccgcg
     1081 tcgcgcgcgt attcgcaatg gaagacctat atgatcagcc cgaaggggga tacgttgaat
     1141 tgtgtggaca gcaaggaccg gaaacagggc cgctgggtaa accatgtcga cgaggtgcgt
     1201 ggcgagccgg gttatgagga ggaggggatg ttcaggaacg accggaaaga aggaacctgg
     1261 cggttatata cgttgcaggg ggatctttcg ggggtagaat tctacaaatg ggggttgaag
     1321 gatggggttt gccagtattt cgggatgaac gggaccctgc agcgggagga gagctggcgg
     1381 gcgctgaacc cggataagga gtatgatacg atacaggtgg aggatgtaga tcacctcgat
     1441 cattataaga ccgtcatcat caaaaatgaa ggcgccgcca tcagggacgg tgtttggaaa
     1501 tactatgatc ccagcacggg gatggtctcg cggcaggaga cctatacgct cggtaaatta
     1561 gagcatcctg cgtcgcagca gacggccgcc tccacggcgc ccaagactgt tgccaagcca
     1621 aaggaagtcc tcgacttcga gaagaagatc gggaagaaga aggtcaaggt ccaggatggg
     1681 agtgtgtatt agttcggtaa aaggaccgaa cttttggatg gttcggtgaa atggccgaac
     1741 tagtggggtg gttcgtataa aatggctaac ttttggaaag ttcggtaaaa tagccgaact
     1801 ttgggcgagt tcgtataaat taccgaaccg aatcgaaatg atgagcctac ccaaagttgt
     1861 ccgtggagct atattgacgg gtgacattgt aaattccacc cagttgctat tggaagaagg
     1921 aaccaaactg gttaccgtgt tgggcctgtt cctggcccat aatccgcatg aattctaccg
     1981 gggcgatagt ttccaggcct atataaagaa gccggatgag gcacttcgac tagcattggc
     2041 atgcaggtgt ctggctatcg gcattacgga ggcgagaagg gaggatacgg cgtcgatagc
     2101 cgatgtcagg atcagtatcg ggataggaga ggtgcccact tcgcttaaaa gccttggtgt
     2161 agccagggga gaggcttttc ttctttccgg ccggcagttc gattcgctgc gggagactgg
     2221 caggcgtctg gccatccgtt gcaacgacaa cgagccgatc gccaacatcg gtttcgaagt
     2281 gatgaccgat tacctggata gtatctgccg gaaaatgacc cccacacagg cgaaccttat
     2341 cgtcgagctg ttgcggggaa cttcccaaca gcaatatgcc aatacattcc agaaatcgaa
     2401 gagtacggtt tcgcagctgg cgaatgctgg caggtggccg gaaatagaga aagtattgaa
     2461 acaatatgaa cagctgatca acctcatcca atgatgccct cacacctttg gcttaccaaa
     2521 ctgatccttt ctcacttatt gacggatttt gttttacaac catccagttg ggtggaagat
     2581 cgcggtaaaa agcattttgc atcccggaaa ctgtacttgc atgctctgat aacggcgggg
     2641 ctggctttta ttttaatcgg ctggcaatat tgg
//
LOCUS       DATGFG010000264         9907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_150783, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000264 DATGFG010000000
VERSION     DATGFG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150783"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..9818
                     /locus_tag="VKQ52_04950"
     CDS             84..9818
                     /locus_tag="VKQ52_04950"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLZ86559.1"
                     /translation="MADADCLLMEMELVTGAERKLLLGEFNDTARPIDREKTLFTLFS
                     EQASRTPNRIAVEHNGTRVSYRELADRATKLSAYLISKGAGAGSRVALYMSRGNTMLL
                     AIVGTMGAGAAYIPIEVEYPKNRVKEILTDSEADIVIVDNGNAAGLVEIQADITTLRE
                     VVCIDDLDQWPVDAFAIPQMAGTDDIAYIIYTSGTTGKPKGAMVHQLGMLNHMCALIG
                     ELRLGEDDAIAQTASCSFDISVWQFLCALLIGGRTCIIDKEKLMDAGGLLKELQEREI
                     TVAEMVPSVLRSLVDEASQAAGKGLPRLRWMIPTAEQISVALVKKWYRCYPSVPLVNA
                     YGPAEASDDVTLYVIGNEVQEGRIIPIGKPIQNIRIYIVDKNMKLCPVGVRGEICIAG
                     VGVGKGYWKNAATTSRVFVENPFLAEEAADYRKLYRTGDMGFYRQDGNIVFTGRGDDQ
                     VKIRGHRIELGEIESHLLQHQDIKEVAMLVREKEGSKHLVAYYVADTPVEENSLRNYL
                     SGRLPDHMVPSFYVQVDRMPLTVNGKLDKKSLPEPMQRRLTSSREEAATEQERLLVGI
                     WSKVLGEVNVGVTDNFFSAGGDSIKSTQIISALRAAGYEASVKDIFIYQTIRELAPRL
                     KNTGIVERPSADPSGHYQAAPGSGRLSSEELDMLQTKYALEDVYPLSPMQEGMLFHSL
                     YEEQGQEYFEQVSCRIEGRLDPGAVEQTMNTILSRYDVLRTVFVHEGYERALQVVLKE
                     RKIDFIYQDYREACRQQEASEVTESYRQRDRSRRFDLDKDALMRLTVLQLGEEEYELI
                     WSYHHILMDGWCMGIILSDFKELYRAHLTGVRPLLAPARPYGEYIGWLQQRRHEEMAA
                     YWQGYLAGYEQPAPIGSLAGKRTDQPYDLRVEELVIGQAHTRSLQRLSVQYGVTINTI
                     LQAAWGILLSKYTNIQDVVFGAVVSGRPAEIAGIESMVGLFINTVPVRVHYTEEDTVA
                     ELLVRLQEAALAGEPHHYHPLSAIQSSSEAGRGLFDHTLVFENYPIADIIRDVDESTM
                     GFRFAGVQVYIQTTYHLSVVVTPGEESRVNFYYNASVFDRGVIQRLAAHFTRVLEQVA
                     GNSRMAVRDIEVVTAEERHRLLYSYNDKAIAYPRNSTVVDLFVQCAEAFPDRTALLYK
                     GSQLTYKELHERSNQLAHYLQARGVGPGTVVGLMMERSMELIIGLLGILKAGGAYLPM
                     EPGTPEKRTRHMLGESRALLLLTDVGGEEINTCPTAGITSQVLPESMAYILYTSGSTG
                     RPKGVMITHGTLANYINWACSQYVRGAAAAFPLFTSVAFDLTVTSLFCPLVTGNTLVI
                     YEDDDNVRLIERIIEDDRVAVIKLTPSHLKIIRDSLAVLRAPKKHLQRFIVGGEELTT
                     ALARDIYELFDENVEIINEYGPTEATVGCMIHRFRPEETTAAVPIGVPAANVCIYLLD
                     RHLQLTATGAVGEIYISGAGVAQGYVGNEELTKERFLEDPFVPGMRMYRTGDLARWLP
                     DGNLEFAGRADEQVKIRGFRIEPGEIANCLRMHEGVKDAVVLVWEREESRYLTAYYVA
                     ETAEASETAEGSEGAAPGAQAEIEADSLRHFLAAMLPDHMIPTFYVQIGGMPLTVNGK
                     LDKKALPEPGFTGGPAYVAPTTKEERVLIVIWEKVLGREKIGISDNFFSVGGDSIKSL
                     QIVSRLYAAGYEASVKEIFLYPTIRELAARLKRIGLAADQSIVTGESRLTPIQQLFFE
                     GAVVSKHHYNQSVLLQFAQGISAGLAEQIAEALQEHHDALRMVFVREAGRVFMQNRGT
                     DMKVSVQEYDLTQSGNDHIELLLRCNDLQAGIGLVEGPLMKLGVFHLRDGSRLLIVIH
                     HLVVDGVSWRVLLEDIETLYRQLSAGSAVSLPQKTAPFLSWSSHLSAYRQSPSFARGR
                     AYWSKRIGKQEPGVPRDHPEGVNIAGESRLETVRLSGLSTQRLLTEAHQPFGTRVTDL
                     LLCGFLLSLRRQYGMSVVQVDMENHGREEIVAGVDMSRTVGWFASICPVLLESTAEEL
                     PQLIKEVKEALRRVPNNGIDYLLLNYTDEEGQMTGTGRSRIVFNYLGQFDADIINKSF
                     TIAVEPTGDEVARSSRNEYEWELLGMVKGGELELSLRFSGAQYKPETIRTLMGHYRSA
                     LESIIDFCCSYGKTELTPSDLTYKDLPVTQLDDLQRQYQIEDIYPLSPMQEGMLFHSL
                     LEPAADHYFVQLVCRLNGTVDIGSVENALNDIIARYAILRTIFLYKGYERPLQLVLKE
                     RVISIDYIDIREEAMVKPADEIVHDYQLQDRTRKFDLSGGELMRLTVLRLTDNEYRLI
                     WSFHHILMDGWCMGIIIQEFGKLYAGNRTGTPILLPPVQAYFRYIEWLEGRDRDSSAN
                     YWREYLEGFETVASLPARELPSSDDLPFKQQVRQMVITASQTRRLRKISSENRVTINT
                     VLQVGWGILLAKYNNASDVLFGSVVSGRPAEIPGVEDMVGLFINTIPVRLQYREGDTA
                     ADLLQQVQSKAFESEPYHYHPLSGIQSLHRAGRELFTHILVFENYPVGEAIKDRSGDD
                     RPHAFTITGAEVFEQANYDLSVIIVPGEELIIRFYYNANKYTADTIGMIAVHLNRVVE
                     QIISDIQVKIGDIEIVTGEEKHQLLYTFNDTIFPYPQQETVISLFEKQAASYPDSIAI
                     EYGGKRISYRQLNNKANSIARLIRGKIPEKGNHAVGMLFHPCAEMIACMLGILKSGGA
                     YVPLSPDATLVRNQYILTDCGAGLLLVQEGLLSEQRVVVPLTKDIESLIVEENDVWDM
                     PFETVPGTVVSKDLLYIIYTSGTTGTPKGVAVMNRGIINMLYFYKHLYQVSMGMNFSH
                     IANISFDASAFEIWPALTHGGCLCIAPLHIRPDPGLIKDWLMERRIEIAFLPTALAPY
                     IIENEWSKKEHALRVINVAGDRLHYSSSKELPFKLYNLYGPTEDSIWTTFTEIQSSAV
                     STHYSIGKPIANKRIYILDKTHKPQPVGIAGELCISGDGLARGYVNNESLTHERFVDD
                     PFFDGGRMYKTGDLARWLPTGDIEFLGRVDNQVKIRGYRVEPGEVESLLARYPLIREV
                     VVAVKERMGEKFLVAYYTSDEQVETAMLRDYLRVYLPDYMMPAFFLELPSLPVTANGK
                     IDRKALPDPEVTRTKDYVTASNDIEEKLLAIWADVLHTDIDKIGINDNFFDVGGHSIS
                     LMTLNSKVNEAFGCTISVASMFKLPTIVSIENFIINGDPHVQKLASRIGEDMDEASAT
                     LQLFKDNILD"
BASE COUNT         2413 a         2351 c         2973 g         2170 t
ORIGIN      
        1 attttacgta ttccaccgcc ttattcaaac ccgaaacgat cgaccggttt accgggtatt
       61 tccggaatat cgtcagggcc gttgtggcgg atgccgattg cctgttgatg gaaatggaat
      121 tggtgacggg ggcagagaga aagctattgc tcggggagtt caatgacacc gcgagaccca
      181 tcgatcggga aaagacatta tttacgttat tcagtgagca ggcatcgcgc acgccgaacc
      241 ggatagcggt ggagcataac ggcaccaggg tcagttatcg cgagctggcg gaccgcgcca
      301 caaagttgtc tgcctacctg atatcgaaag gcgccggggc cggcagcagg gtggcgctat
      361 atatgtcccg gggcaatacg atgctcctgg ccatagtagg caccatggga gcgggagcgg
      421 cctatatacc tattgaagtg gaatacccga agaaccgggt gaaggagata ttgaccgaca
      481 gtgaggcgga cattgttata gtggataacg ggaatgcggc cggcctggtg gaaatacagg
      541 ccgatataac gaccctgcgg gaagtagttt gcatcgatga tctggatcaa tggcctgtgg
      601 atgcctttgc cataccgcag atggccggta ccgatgatat tgcctacatc atttacacgt
      661 cgggtactac cggcaagccc aagggggcaa tggtgcacca gctgggtatg ctgaaccata
      721 tgtgtgcgtt gatcggggaa ctgcggctcg gtgaggatga cgccattgct cagacggcat
      781 cctgcagctt tgacatatcg gtatggcagt ttttgtgcgc cttgcttatc ggagggcgca
      841 cctgtatcat cgacaaggaa aaattgatgg atgccggtgg cttgttgaag gaattgcagg
      901 aaagggaaat aaccgtggcc gaaatggtgc cctccgtatt gcgatccctc gtggatgagg
      961 cgtcgcaggc agccggcaag ggcctgcccc gcttacgctg gatgatccct actgccgaac
     1021 agatcagcgt ggcattggta aagaaatggt accggtgtta cccctccgta ccgttggtaa
     1081 acgcctatgg tccggcggag gcctctgatg atgtaaccct gtatgtgatc gggaatgaag
     1141 tgcaggaggg gcgcatcatc cccataggaa agccaatcca gaacatacga atatatatcg
     1201 tggataagaa tatgaagctc tgcccggtgg gggtccgcgg tgagatatgt atcgcagggg
     1261 tcggggtcgg gaaagggtat tggaagaatg cggcaacgac aagccgggtg tttgtggaaa
     1321 acccttttct cgcagaagaa gcggcggatt accggaaact gtatcgcacc ggcgatatgg
     1381 gcttttaccg gcaggacggc aatattgtgt ttaccggtcg tggtgacgac caggtaaaga
     1441 tcaggggaca ccgtatcgaa ttgggtgaga tcgagagtca tcttctgcag caccaggata
     1501 taaaggaagt ggcgatgctc gtacgggaaa aggaagggag taaacacctg gttgcgtatt
     1561 atgtggcgga tacccctgtt gaagaaaatt cgctgcgcaa ttatttgtcc ggcaggctcc
     1621 ctgatcacat ggtgccttct ttttatgtac aggtagaccg gatgcccctg acggtgaacg
     1681 gtaaactgga taaaaaatca ctgcctgaac caatgcagcg gcgactcacc tcttccaggg
     1741 aggaggcggc aacagaacag gaaaggctgc tggtgggcat atggtcgaag gtgttggggg
     1801 aggtaaatgt gggggtaaca gacaatttct tttcagcggg tggcgattcc atcaagtcta
     1861 cacagatcat atccgcctta agggccgccg ggtatgaagc atcggtgaaa gatatcttca
     1921 tttatcaaac catccgggaa ctggcgccaa gattgaaaaa taccggtatt gtggaacggc
     1981 cgtcggcaga cccatcaggc cattaccaag cggccccggg atccggaaga ctgtcatcgg
     2041 aagagcttga tatgttacag acgaaatacg ccctggagga tgtgtatccc ctgtcaccga
     2101 tgcaggaggg tatgttgttc cactcgctgt acgaggagca ggggcaggag tattttgaac
     2161 aggtgagctg ccgtatagaa gggcggcttg atccgggggc tgtagaacag accatgaata
     2221 ccatcctgtc ccgctacgat gtgctgcgga cggtgtttgt acatgaagga tatgaacgcg
     2281 ccctgcaggt ggtgttgaaa gagaggaaga tcgactttat atatcaggac taccgggagg
     2341 cctgccggca gcaggaggcg tcggaagtga cggagtcata ccggcagcgg gaccgatcgc
     2401 gcaggttcga cctggacaag gatgcgttga tgcggctgac ggtattgcag ctgggggagg
     2461 aagaatatga acttatctgg agttatcatc atatcctgat ggatggctgg tgcatgggta
     2521 tcatactatc ggatttcaaa gaactgtaca gggcgcacct gacgggtgta aggcccctgc
     2581 tggcgccggc caggccttat ggggaatata tcggctggct gcagcagcgg cggcacgagg
     2641 agatggcggc ttactggcag ggatacctgg cgggctatga gcagccggcg cctatcggca
     2701 gcctggccgg caagcggaca gaccagccgt atgatctgcg ggtagaggaa ctggtgatcg
     2761 ggcaggccca cacccggtca ctgcagcgac tgtctgtgca atacggcgtt accatcaata
     2821 ccatcctgca ggccgcatgg ggtatcctgc tgtcgaagta tactaatatc caggatgtcg
     2881 tatttggtgc ggtggtatcg ggcaggccgg cggagatcgc cgggatagaa tcgatggtcg
     2941 ggttgttcat caacacggtg ccggtgcgcg tccattatac ggaggaggat acggtggctg
     3001 agctgctggt caggctgcag gaggcagcgc tggcgggtga gccgcatcat taccatccgc
     3061 tgtcggcgat ccaatcgtcc agcgaagccg gccgcggttt gttcgatcat accctggttt
     3121 ttgaaaatta cccgatagcc gatatcatca gggatgtcga tgaatccacg atgggctttc
     3181 gttttgcggg ggtgcaggta tatatacaaa cgacctatca tctgtcggta gtagttacac
     3241 cgggcgaaga gagccgggtt aacttttatt ataatgccag tgtgttcgac cgtggggtca
     3301 ttcagcgcct ggcagcccat tttaccaggg tgctggagca ggtggccggc aacagccgaa
     3361 tggcggtgcg tgatattgag gtggtgacgg cggaagaaag acatcggtta ctttattcct
     3421 ataacgataa ggccatcgcc tatccccgca acagcaccgt tgtcgatctg tttgttcagt
     3481 gcgcggaggc cttccctgac agaacggccc tcctgtacaa gggatcgcag ctgacctata
     3541 aggagctgca tgaacgatcg aatcagctgg cgcattattt acaggccagg ggggtagggc
     3601 ccggaaccgt cgtgggcctg atgatggaac gatcgatgga actgatcatc ggtctgctcg
     3661 gtatcctcaa ggccgggggc gcctacctgc cgatggaacc cggcactcct gaaaaacgca
     3721 cccggcatat gctcggggaa agccgggcgt tattgcttct aaccgatgtc ggtggcgagg
     3781 agatcaacac ctgcccaacg gccggcatca ccagccaggt actgcccgaa agcatggcct
     3841 atatactgta tacctccggt tcgacgggta gaccgaaggg ggtgatgatc acacacggca
     3901 ccctggccaa ttacatcaac tgggcctgta gccagtatgt caggggggca gcggcggcat
     3961 tccccctgtt cacctccgtc gcctttgatc ttacggtcac ctcccttttc tgcccgctcg
     4021 tcaccggcaa taccctggtg atctatgagg atgacgataa tgtccggctg atcgaaagga
     4081 tcatcgaaga cgaccgggtg gccgtcataa agcttacccc ctcccattta aagatcatcc
     4141 gtgattccct ggcggtgtta cgcgctccca aaaagcacct ccagcgtttc atcgtgggcg
     4201 gcgaggaact gacgacggcc ctggcaaggg atatttatga gctgttcgat gaaaacgtgg
     4261 agatcatcaa tgaatatggg cccaccgagg ctaccgtcgg ttgtatgatc caccggttcc
     4321 gcccggagga gacgacggct gccgtaccga tcggggttcc ggcagccaat gtatgcatct
     4381 atttactgga caggcatctg cagctgaccg ccaccggcgc cgtcggcgag atctatatct
     4441 ccggggccgg tgttgcacag ggatatgtcg gcaatgagga gttaacaaag gaacgattcc
     4501 tcgaagaccc cttcgtcccg gggatgcgga tgtacaggac cggtgacctg gcgcgctggt
     4561 tacccgatgg gaacctggag ttcgcgggaa gggcggatga acaggtcaag atacggggct
     4621 tccggataga gccgggggag atagccaatt gtttgcgcat gcacgaaggg gtgaaggatg
     4681 cggtagtcct tgtatgggaa agggaggaaa gcaggtacct caccgcttat tatgtagcgg
     4741 aaacagcgga agcatcggaa acagcggaag ggtcggaagg agcagcgccc ggggcacagg
     4801 cggagatcga agcggatagc ctgcggcatt tcctggccgc catgctgccg gaccatatga
     4861 tcccgacctt ctatgttcag atcgggggga tgcccctgac cgtgaatggc aagctcgata
     4921 aaaaggcgct tccggaaccg ggatttacgg gcgggccggc gtatgtcgcg ccaacaacaa
     4981 aagaagaaag ggtattgatc gttatctggg aaaaggtgct gggaagggaa aagatcggca
     5041 tatccgacaa tttcttttct gtaggagggg attctattaa atccctgcag atcgtctccc
     5101 ggctgtatgc cgcaggctat gaggcatccg taaaggagat cttcctttat cccaccatcc
     5161 gggaactggc ggcaagactg aagcggatcg gcctggcggc agatcagtcg atagtgacgg
     5221 gggaatccag gctgaccccc atacagcaat tgttcttcga gggggcggtg gtaagcaagc
     5281 atcactataa ccagtcggtg ctgctgcaat tcgcacaggg catcagtgcc gggctggcgg
     5341 aacagatagc cgaggcactc caggagcatc atgatgccct gcgcatggtg ttcgtgcggg
     5401 aggccggcag ggtatttatg cagaaccggg gaacagatat gaaggtgagt gtgcaggagt
     5461 atgaccttac ccaaagcggt aatgaccata ttgaactatt gctgcgctgc aatgacctgc
     5521 aggcgggtat cggcctggtg gagggtccgt tgatgaagct gggggtattc cacctcaggg
     5581 atggcagccg gttgctgatc gtgatccatc acctggtggt ggatggggtg agctggcggg
     5641 tcctgctgga agatatagaa acgctttacc ggcagctgtc agcgggatca gcggtctccc
     5701 tgccacagaa gacggccccg ttcctgtcct ggtcgtcgca tctgtcggcc taccggcaga
     5761 gcccctcctt tgccaggggc cgggcctact ggtcgaagcg gatcggcaaa caggagccgg
     5821 gtgttccccg ggatcatccc gaaggggtca atattgccgg ggagagccgg ctggagacgg
     5881 tgcggctgag tggtctttcc acacagcggc tgctgacgga agcacaccag ccctttggga
     5941 cgagggtcac cgacctgctg ctgtgcgggt ttttgctcag cctccgcaga cagtatggga
     6001 tgagtgtggt gcaggtggac atggagaacc atgggcggga agagatcgtg gcaggggtgg
     6061 atatgagccg gacggtgggg tggtttgcga gcatctgtcc ggtgttgctg gagagcacag
     6121 cggaggagct gccgcagctg atcaaggaag taaaagaggc cctgcgccgt gtccccaata
     6181 atgggatcga ctatttgctc ttgaactata ccgatgagga ggggcaaatg accggcacgg
     6241 gccggagccg tatcgtcttc aactacctcg gccagttcga cgccgatatc ataaacaaat
     6301 cgttcaccat tgctgtggaa cccaccggtg acgaggtggc ccgcagttcg aggaacgaat
     6361 acgaatggga gctattggga atggtaaaag gcggtgagtt ggagctaagc cttcgtttca
     6421 gcggcgccca gtacaaaccg gaaacgatca ggacgctgat ggggcattac cgatcggcgc
     6481 tggaatcgat catagacttc tgctgcagtt atggaaagac ggaactgacg ccatccgatc
     6541 ttacctataa ggatctgccc gttacacaat tggatgacct gcagcgacaa tatcagatag
     6601 aagacatcta tcctttgtcg ccgatgcagg aaggcatgct tttccattcc ctgcttgaac
     6661 cagcggccga tcattatttt gtgcaactgg tttgccggtt gaacggaacg gtggacatag
     6721 ggtctgtcga gaatgcgttg aatgatatca tcgcccgtta tgccatcctc cggacgatat
     6781 tcctgtataa aggctatgag cggccattgc agctcgtact gaaggaaagg gtcatctcga
     6841 tagactatat cgatatccgg gaggaagcga tggttaaacc cgcagatgaa atagtccacg
     6901 actatcagct gcaggaccgt accaggaaat ttgatctgtc ggggggcgaa ctgatgcgcc
     6961 tcactgtcct ccgtttgaca gataatgaat accggcttat ctggagcttt catcatatcc
     7021 tgatggatgg ctggtgcatg ggtattatca tacaggaatt cgggaaactg tatgcaggca
     7081 acagaacggg aacccccatc ctgttaccgc ctgtccaggc ctatttccgg tatattgaat
     7141 ggctggaggg ccgggatagg gacagctccg cgaattactg gcgtgaatac ctggaaggct
     7201 tcgaaactgt ggccagcctg ccggcaaggg agctgccgtc gtcggacgat ctgcctttta
     7261 aacagcaggt ccggcagatg gtaataacag catcgcagac acgtcgcctg cggaagatct
     7321 cctcggaaaa cagggtgacc ataaataccg tattgcaggt ggggtggggc atattactgg
     7381 cgaaatacaa taacgcaagc gatgtgctgt ttggctctgt agtgtccggc aggccggcag
     7441 aaataccagg tgtagaggac atggtgggtc tgtttatcaa tacgatcccg gtgcgattgc
     7501 agtacaggga aggcgatacg gctgccgatc tgttacaaca ggtgcagtcg aaggcatttg
     7561 agagcgagcc atatcattac catccgctgt ccggcatcca gtccttgcat agggctggcc
     7621 gggaattgtt tacgcatatc ctggttttcg agaactaccc ggtaggcgag gcaataaaag
     7681 accgttcagg cgatgacagg ccgcatgcgt ttaccatcac cggtgcggag gtattcgaac
     7741 aggcgaacta tgatctatcg gtcattattg ttccgggaga agagctgatc atcaggtttt
     7801 attacaatgc caataaatat accgccgata cgatcgggat gatcgccgtt catctcaatc
     7861 gcgtagtgga acagatcata tcggatatcc aggtcaaaat cggggatatc gagatcgtta
     7921 ccggtgagga gaagcatcaa ctgttgtata cattcaacga taccattttc ccctacccgc
     7981 aacaggaaac cgttatatcc ctatttgaaa agcaggcggc gagctacccc gacagcattg
     8041 ccatcgagta cggtgggaaa aggataagct acaggcaact aaataataag gccaattcaa
     8101 tcgcccgttt aataaggggt aaaattcctg aaaaaggaaa tcatgcagtg gggatgttat
     8161 tccatccctg tgccgaaatg attgcctgca tgctgggcat cttaaaatcc gggggcgcct
     8221 atgtacccct gtcaccggat gccacgctgg tcagaaatca atatatactc acggattgcg
     8281 gggcgggcct tctgcttgtc caggaaggtc tcctgagcga acagcgagtg gtcgtcccgc
     8341 tcacaaagga tatcgaaagc ctgatagtag aggagaacga tgtctgggat atgccatttg
     8401 agactgtacc gggaacggtt gtatcgaaag accttcttta tatcatctat acttcgggta
     8461 ctacgggtac gccaaaaggg gtcgcggtaa tgaatcgcgg aattatcaat atgctgtact
     8521 tctataagca tttgtaccag gtgtccatgg gtatgaactt cagtcatatt gccaatatat
     8581 cgttcgacgc ctctgcattt gaaatatggc cggccctgac ccatggcgga tgtctatgca
     8641 ttgccccgct tcatatacgg ccggacccgg gtctgatcaa agactggctt atggagcgtc
     8701 gcattgaaat agccttcctg ccaactgcat tagctccgta tataattgaa aatgaatgga
     8761 gtaaaaaaga gcatgcgctc cgggtcataa atgtcgccgg cgaccggcta cattattctt
     8821 catccaaaga attacctttt aaactgtata acctgtatgg gcctacagag gattccatct
     8881 ggacgacttt cactgagata caatccagtg ctgtcagtac acactacagt atcgggaagc
     8941 caattgccaa taaaaggatc tatatcctcg ataagaccca caagccgcag cctgtaggca
     9001 tcgccggtga attgtgtatc tcgggtgacg ggttggccag ggggtatgtg aataatgagt
     9061 cgctaaccca tgaaagattt gtcgatgatc cttttttcga cggcggaaga atgtataaaa
     9121 cgggcgacct cgccagatgg ctgccgacgg gggatatcga atttttggga agagttgata
     9181 atcaggttaa aataagaggc tatagagtag aaccaggcga ggtggagagc ctgctcgcca
     9241 ggtatcccct tatcagggaa gtggtggtgg cggtgaaaga acgaatggga gagaaattcc
     9301 tggttgccta ttatacttcg gatgaacagg tagaaaccgc catgctaagg gactatttgc
     9361 gggtctatct cccggattac atgatgccgg ctttcttttt agaattacca tcactgcccg
     9421 tcacggccaa tggcaagatc gacagaaagg ccttgccgga tcccgaagtt accaggacga
     9481 aagactatgt aactgcgtcc aatgatatcg aagaaaagct attggccatc tgggcggatg
     9541 tattacatac cgacatagac aagatcggaa ttaacgacaa cttcttcgat gtgggcgggc
     9601 attcgatcag tttaatgacc ttgaacagca aggttaacga ggcctttgga tgtaccattt
     9661 ccgtagccag catgttcaaa ttgcccacta tcgtgagtat tgagaacttt attataaatg
     9721 gggaccccca tgtgcaaaag ttagcgagcc gaatcgggga ggacatggac gaggcaagcg
     9781 ctacactgca gttgttcaag gataatatac tggactaatc aaatcagtaa cctaaaatca
     9841 tctgttatga acgatctatt caatttaacc ctggtagaat ccggggaaca ggttgaccag
     9901 ataaata
//
LOCUS       DATGFG010000265         4789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_835419, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000265 DATGFG010000000
VERSION     DATGFG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835419"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..674
                     /locus_tag="VKQ52_04955"
     CDS             228..674
                     /locus_tag="VKQ52_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86560.1"
                     /translation="MSLSIKTFALIALLLTVSSSYATPNDDSNGAVKTSFRKDFKTAE
                     LMGTEVKPNFTKLTFKMNDVIMYAFYSDNGQLLAVTRNITSNQLPIQLLLDVKQNYSE
                     YWITDLFELTGDGASNYYITVENADTRVTLRSNGSDSWDVYEKKAK"
     gene            complement(823..1302)
                     /locus_tag="VKQ52_04960"
     CDS             complement(823..1302)
                     /locus_tag="VKQ52_04960"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HLZ86561.1"
                     /translation="MITEKDILYLQQAVALAREGVEKGMGGPFGCVIVRNGEIVGKGC
                     NGVTSSNDPTAHAEIVAIRDACQRLGDFQLADCDVYTSCEPCPMCLGALYWARPRRVV
                     YAATRHEAAEAGFDDAFIYREINLPDGERRIPFVHQAVEATRELFGFWVEMGNKKLY"
     gene            complement(1411..2346)
                     /locus_tag="VKQ52_04965"
     CDS             complement(1411..2346)
                     /locus_tag="VKQ52_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86562.1"
                     /translation="MNAVVLKMIERKTGVPGIAALLADRLSGSELNSLLLEVYGRRSE
                     KMQPAELLAQYRLNRLVHPSGLDKIALLESGLVALRFFRERGFLPVELSPVAVFGSCS
                     VIAAVSQDKVVSASRNTEVVADATNSLALHIADVRKAGEDGPLRFCTVHRHVRAQRFA
                     DKRYLPHFMIGCMVSADRDRGSYGFECEGLAEHVGNYIDLLRDGYGVGEFRLLLKKRG
                     GYDERSPLIGRVEAFLRDRLKGVVVDQEEAPVESSYYKGVQFKLYIAVNGVEWEIADG
                     GFVDWTQQLLGNRKERLLISGIGLEFLFKMQRGLT"
     gene            2669..4297
                     /locus_tag="VKQ52_04970"
     CDS             2669..4297
                     /locus_tag="VKQ52_04970"
                     /inference="COORDINATES: protein motif:HMM:NF039744.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="HLZ86563.1"
                     /translation="MLRRSLSSLFLIALILTARCQDPTNPSLTTLDQAIDASPKYDTD
                     KQKGIQQLRTALAATPKENLTALFDNYLRLYEQYKVFNYDSAFAYARALQATALQLGD
                     PVKTTYARLKLGFCLLSSGLFKETLDSLLEIRIAGAPDSLQAEYYALMGRYYYDLGDF
                     DNDGYNTPAYTRKGNAYMDSSLRLYPPGSFNASYYKGLRDIKSGQGSQALAEFRQLLQ
                     RPGLTLHQLAVTTSTLSDIYITNGQPDTAIRLLIRAAIADIQSSTKETAAIFNLAQLL
                     YKKGDVRNAASYIEVAIRDAVFYGARQRKVQVSAILPLIEGEKVSRVEEQKSIFVTWS
                     IISTFLLLVVVVLAITVYRQVRRLKLAQAIITKAHIKEQAINQQLLETNNKLSEANKI
                     KEEYIGYFFNADSESFAKIEKFKKSLEQKIAYRKWDDIITLVNNTNLKKEKEELLQNF
                     DKVFLRLFPDFIKQFNALFRPEDQVEGKDNEFLNTDLRIFALIRMGIHENEKIARILE
                     YSVNTINTYKTRIKNRSIVPNEEFEQRIMEIKTV"
     gene            complement(4387..>4789)
                     /locus_tag="VKQ52_04975"
     CDS             complement(4387..>4789)
                     /locus_tag="VKQ52_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HLZ86564.1"
                     /translation="YSPLQMAADLPENYEAHADAFQFIREVPVDWDDTKILEAEPGDY
                     LTIARKEKGKDNWFLGAITDEHARTMSVPLAFLDKGRKYKATVYKDGAGADWKANPEV
                     YAIETLTVDSKSVLTVKLAPGGGCAVSIIPL"
BASE COUNT         1244 a         1677 c          886 g          982 t
ORIGIN      
        1 cccgtacccc cctgatcacc ccatcagccc caactaccaa ccccggcggc caaccccaaa
       61 acccttcatc cagcctttcc taccgcttaa cacagtattg ggccattcgt agtactatgt
      121 attgtaaagt acctgttctg cgcattatct ttgttatgca agccaatcgg aaaagaacaa
      181 aacaaaccaa gtcaaccaat tttttcaaaa ttataaaacc taagtatatg tcactctcaa
      241 tcaaaacctt cgccctcata gccctgctcc ttacagttag cagcagttac gctaccccca
      301 atgacgacag caacggtgca gtcaaaactt ccttccggaa agacttcaaa acagctgaac
      361 tcatgggaac cgaggtcaag cccaatttta ccaagctgac ctttaaaatg aacgacgtca
      421 ttatgtacgc cttctactcg gacaacggtc agttactcgc cgtcacccgt aacatcacct
      481 ccaaccaact gcccatccag ttgctgctgg acgtaaaaca gaactactcc gaatactgga
      541 tcaccgatct gttcgagctc accggcgacg gcgccagcaa ctactacatc accgtagaga
      601 atgccgacac ccgtgtcacc ctgcgctcca acggctccga cagctgggac gtatatgaga
      661 aaaaagcaaa ataattatag cctacctatc caaacacccc ctaccggctc cgctcaactg
      721 cggagccttt tttttgccgg taacgactcc gatccaccca caacaactcc gatccgccgg
      781 caactcctaa caactccgat tcacccccgc ccccaatatt ctctaataca atttcttatt
      841 ccccatctcc acccaaaacc caaacaactc ccgcgtggcc tccaccgcct ggtggacaaa
      901 ggggatccgc cgctccccat ccggcaaatt gatctcccgg taaataaacg catcgtcaaa
      961 ccccgcctcc gccgcctcat gtcgcgtagc cgcatacacc acccgccgcg gcctcgccca
     1021 atacagtgcc ccaagacaca tcggacaagg ctcacaggac gtatacacat cgcaatcggc
     1081 aagctggaaa tcccccagcc gctgacacgc atcccggatc gcaactattt ccgcatgagc
     1141 cgtcggatcg ttgctggacg tcaccccatt acaccccttc cccacaatct ccccattcct
     1201 gacgatcaca caaccaaacg gcccccccat ccccttctcg accccctccc gcgccagcgc
     1261 taccgcctgc tgcaaataca gaatatcctt ttcagtgatc atatatttat tctccaaata
     1321 tcgaatttcg gcaaaaaatt gcccgcaaaa taatttttag ccgaaacacc cacaatgtct
     1381 gccctcgcgg gcgaaccgga ttccatccgg ttaggtcaat ccccgctgca ttttaaacag
     1441 gaattccaac ccaatcccac tgatcagcaa ccgctccttc cggttaccga gcaattgctg
     1501 cgtccaatcc acaaaacccc catcggcgat ctcccactcc acgccgttga ctgcgatata
     1561 caacttgaac tgcaccccct tataataact gctctccacc ggcgcctcct cctggtcaac
     1621 aacaactccc ttcaaccggt cccgtaaaaa ggcctccacc ctcccgatca gcggactgcg
     1681 ctcgtcatat cccccgcgtt tcttcagcaa caagcgaaat tcccctactc catatccatc
     1741 ccgtaacaga tcgatataat tcccaacatg ttccgccagt ccctcacact cgaacccgta
     1801 acttccccga tccctgtccg cggaaaccat acacccgatc ataaaatgag gaagataccg
     1861 cttgtcggca aatcgctgcg cccgcacatg ccggtgcacc gtgcagaaac gcaacggccc
     1921 atcctcccca gcctttcgca catcggcaat atgcagggca agggagttcg tcgcatccgc
     1981 taccacctcc gtattcctcg acgccgagac caccttatcc tggctcaccg ccgcgatgac
     2041 cgaacaactg ccaaagaccg caaccggcga caattccacg ggcaggaacc cccgctcacg
     2101 aaaaaaacgc aacgccacca gcccggattc cagcagggcg atcttatcca accccgacgg
     2161 atggacaagc cggttcagcc ggtactgtgc caagagctcc gccggttgca tcttttccga
     2221 tctccgccca taaacctcca gcaacaggga atttaactcg ctcccggaca accgatcggc
     2281 cagcagggca gcaatccccg gcaccccggt cttcctttcg atcattttta aaacaactgc
     2341 attcataaac taacggtctc ccttagctaa atgtaccaat ttggaggcac aacgcctatc
     2401 cctacctccc tcatggcccc gaattgggcc cgattctaca ccccgctgag gaaattactc
     2461 cccagacgtc gatacatttc ctcaattttc aggcgcaaaa atcccgcgct ccgtttagac
     2521 cggatgtgga ccgttgtttg cattactatt taggctttta ataggatatt ggattttagc
     2581 aacggcctgg atttttatct gggcttcttt tttttacgga tatctttgat cgtcggtatc
     2641 tttgatcgtc caaaactact ttgccaacgt gctccgccga tcgctttcca gtctcttctt
     2701 aattgcgctc atcctcaccg cccggtgcca ggatcctact aaccccagcc tcaccaccct
     2761 ggatcaggcc atcgacgctt ccccgaaata tgacacggat aaacagaaag gcatccagca
     2821 gttgagaacc gcactcgccg caacaccaaa agagaacctc accgccctct tcgacaacta
     2881 cctccgcctc tatgaacaat acaaggtctt taattatgat tccgccttcg cctatgcccg
     2941 cgcactccag gcaaccgccc tccaattagg tgatcccgta aaaacaacct acgcccgttt
     3001 gaagctgggt ttctgtctcc tttcctccgg gctcttcaag gaaaccctcg actccctcct
     3061 ggagatccgc atcgccggtg cacccgacag cctgcaggcg gaatactatg cgctcatggg
     3121 acggtactac tatgatctcg gcgatttcga taacgacggc tataataccc ctgcctatac
     3181 ccgaaaaggc aacgcctata tggactcctc cctgcgcctc taccccccgg gctccttcaa
     3241 tgcctcctac tataaaggcc tccgggacat aaaatccggt cagggctcac aggccctcgc
     3301 cgaattccgg caactgctcc aacgccccgg cctaacgctc caccaactgg ccgtcaccac
     3361 ctccacgctg agcgatatct atatcaccaa cggccagccg gacaccgcca tccgcctgct
     3421 catccgcgcc gctatcgccg acatccaatc ctcgacaaag gaaacagcag ctatcttcaa
     3481 tctggctcag ctcctctata aaaaaggcga tgtgcgcaac gccgcctcct acatcgaggt
     3541 agccatccgc gacgccgttt tctacggggc ccgccagcgg aaagtacagg tcagcgccat
     3601 cctccccctc atcgaagggg aaaaagtaag ccgcgtcgaa gaacaaaaaa gcatcttcgt
     3661 cacatggtcc atcatctcca ccttcctcct gctcgtggtg gtcgtcctcg ccatcaccgt
     3721 ctacagacag gtccgccggc tcaaactggc ccaggccatc atcacaaaag cccatataaa
     3781 ggaacaggcg ataaaccagc agctgctgga aaccaataac aaactctccg aagcaaataa
     3841 gataaaggaa gaatacatcg gctatttctt caatgccgac tcggaatcct ttgcaaagat
     3901 cgagaagttc aaaaaatccc tcgagcaaaa gatcgcctac cgcaaatggg atgacatcat
     3961 caccctcgtc aacaacacca atctgaaaaa ggaaaaagaa gagttactgc aaaacttcga
     4021 caaggtcttc ctccggctct tccccgattt tataaaacag ttcaacgccc tcttccgccc
     4081 cgaagaccag gtcgaaggaa aagacaacga attcctcaac accgacctcc gcatcttcgc
     4141 cctcatccgc atgggcatcc acgagaacga aaagatcgcc cgcatcctcg aatactccgt
     4201 caacaccatc aacacgtaca aaacaaggat aaagaaccgg tccatcgtcc ccaacgaaga
     4261 attcgaacaa cggatcatgg agatcaaaac cgtatagaaa agcgaaaaat gatacgattt
     4321 gatacgagaa tatacgatcc tgatcctctt cccaccatta acttgggcgc ccgcggcagc
     4381 gccgccctat aatgggatta tactcaccgc acacccgccc cccggcgcca gcttcaccgt
     4441 caacacactc ttactatcca ccgtcagcgt ctctatcgca tacacctccg ggttcgcctt
     4501 ccaatccgcc ccggccccat ccttatagac ggtcgcctta tacttccggc ccttatcgag
     4561 aaacgccagc ggcacactca tcgttcgcgc atgctcatcc gtaatagccc ccaaaaacca
     4621 attgtccttc cccttctcct tccgcgcaat ggtcaggtaa tcgcccggct ccgcctccag
     4681 gatcttcgta tcatcccaat ccaccggcac ctcccggatg aactgaaaag catccgcgtg
     4741 cgcctcataa ttctccggca gatcggccgc catctgcaac gggctatat
//
LOCUS       DATGFG010000266         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1035731, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000266 DATGFG010000000
VERSION     DATGFG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035731"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="VKQ52_04980"
     CDS             <1..1727
                     /locus_tag="VKQ52_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86565.1"
                     /translation="QHTAPAAAPEQHAATERRAVAATDPSPKIVNIVNFIRLLEPRDS
                     AITEDVLYQTVVNQVALLRKYRLGGTFLLQYDALMDHRYQSLLRSLPRDSFEIGAWWE
                     IPQPLVEKAGLPWRGRYPWDWRANIGFATGYTPAEREKLIDTYMADFKKIFGYYPASV
                     ASWFIDAHSLQYLYEKYHIQASANCKDQYGTDGYTLWGGYWNQAYYPSKTNSYMPAQH
                     AANQIPVPIFRMLGSDPVRQYDNGLGQSRQGVVTLEPVYAFGGGDSTWVDWFFRSFTG
                     TRELGFNYTQAGQENSFTWAAMAKGLQLQMPLIARLRDEHRIRVLTLGESGRWFRNRY
                     RLTPATTFTVTRDLPGSDRRTIWFDSRFYRVNLLWEKGTLRIRDIHLFDENLPSIYET
                     RPATANECSFFTLPVVDGYLWSKGDRLAGLRLMAVIDGKATLVQGGEPLFKAIADSTM
                     QITWPLTTAKGALVITLRTKQLEIKWQDGPPVDWFFELQTAEGAKLPFDTIRPQRLDC
                     HFGNTKYHLRAEKGSFSQPADPVNPATPASLTNPTTPANSTTPANPTGGAAWRLHPQA
                     NTIILKFD"
     gene            complement(1714..2463)
                     /locus_tag="VKQ52_04985"
     CDS             complement(1714..2463)
                     /locus_tag="VKQ52_04985"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLZ86566.1"
                     /translation="MSKLQNKTAVITGGSSGIGLAAAKRLVEEGAYVFITGRRAAELE
                     KAKKEIGRNVSAVQGDVASLADLDRLYATVKAERGGIDILVINAGIVEMSTLAAATPE
                     HFDKIFNVNVRGVFFSANKALPLLRDGGSVVLVSSAVQFKGFPFYGVYAATKAAMRSF
                     TRSWAAELKERRIRVNSLSPGPVETPIIDSQFKTKAEGDAIRAQFAQMVPMGRIGNAE
                     EMAAAIYFLASEESSYSTGIDLVADGGFSQI"
     gene            2536..3159
                     /locus_tag="VKQ52_04990"
     CDS             2536..3159
                     /locus_tag="VKQ52_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ86567.1"
                     /translation="MSPNKNPLIKQSSMDTFVTYLSSRATFSPKEMQQILAASTLLRL
                     HRKDLLLRQGEVCRGYAFVNKGCFRLYRIGGDGAEHILRFAIENWWMSDMESVDLRQP
                     SKCTIEALEDSEVLFWTKEDMGALTQKIPALGVVCDQLKLRNQHVNEQRIFAALSYTA
                     EEKYHEFITTYPDLFNRVPLHMVASYLGITRKTISRIRAKDAHPHRS"
     gene            3213..>3627
                     /locus_tag="VKQ52_04995"
     CDS             3213..>3627
                     /locus_tag="VKQ52_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86568.1"
                     /translation="MRTLAIISILLIPAMGLCQETPAKPTPQKDIIDVGRSVLKIHPR
                     VRQNSRKSIYFSILPTSSQAAGPAGLSITATTAAFNLGDDGTGFLSTVTFAPYVATHG
                     RIGFALRSNLWLPHNTWDILGDSRLLYYPQETWGLG"
BASE COUNT          787 a         1257 c          907 g          676 t
ORIGIN      
        1 cacaacacac cgcacccgct gccgcacccg aacaacacgc cgccaccgaa cgccgcgccg
       61 tcgccgcaac tgacccctcc ccgaagatcg tcaacatcgt caatttcatc cggctgctgg
      121 aaccaaggga ttcggccatc acggaagatg tcttatacca gaccgtcgtc aatcaggtgg
      181 cgctcctgcg gaaataccgg ctcggcggaa cctttctcct gcaatacgac gcccttatgg
      241 atcaccgtta ccagtccctg ctgcgcagcc tgccaaggga cagcttcgag atcggcgcct
      301 ggtgggaaat tccccagccc ctggtggaga aggccggcct gccctggcgc gggagatacc
      361 cctgggactg gcgggcgaac atcgggttcg cgacaggcta taccccggca gaacgcgaaa
      421 agctgatcga tacctatatg gcggatttca agaagatctt tggctactat cccgcctccg
      481 tggcttcctg gttcatcgac gcgcacagcc tgcaatacct ctatgagaaa taccatatcc
      541 aggcctccgc caactgcaag gaccagtatg ggacggacgg ctacacctta tggggaggtt
      601 actggaacca ggcctattac ccgagtaaga cgaattccta tatgccggcc cagcacgcgg
      661 cgaaccagat cccggtaccc atcttccgga tgctcggcag cgacccggtg aggcaatatg
      721 ataacggtct cggtcagtcg agacagggag tggtcaccct cgaaccggta tatgcctttg
      781 gcggcggaga ctccacctgg gtggactggt tcttccggag cttcaccggc acccgggagc
      841 tgggcttcaa ttatacccag gcaggccagg agaattcctt cacctgggcc gccatggcaa
      901 aaggcctgca actgcagatg ccgctcatcg cccgcctgcg ggacgaacat cgcatccggg
      961 tgctgaccct gggcgagtcg ggtcgctggt tcaggaacag gtaccggctg accccggcca
     1021 ccacctttac ggtcacccgc gacctgccgg gcagcgatcg ccgcaccatc tggttcgaca
     1081 gccgcttcta tcgcgtaaac ctcctctggg agaaggggac cctccgcatc cgcgacatcc
     1141 atctcttcga cgagaacctt ccctccatct atgagacccg gcccgccacg gccaatgaat
     1201 gttctttctt cacactgccc gtagtggatg gctatctatg gagcaaaggc gatcgcctcg
     1261 ccggtctgcg gctgatggcg gtcatcgacg gcaaggccac attggtgcag gggggcgaac
     1321 ccctgttcaa agcgatcgcc gattccacca tgcagatcac ctggccgctg acaacggcaa
     1381 aaggcgccct cgtcatcacc ctgcgcacaa aacagctcga aataaaatgg caggatggtc
     1441 cccccgtcga ttggttcttt gaattacaaa ccgccgaagg ggccaaactg cctttcgata
     1501 ccatccggcc tcaacgcctg gactgccatt tcgggaatac gaagtatcac ctgcgggcgg
     1561 aaaagggctc cttctcgcaa ccagccgatc cggtcaaccc ggccaccccc gccagcctca
     1621 ccaacccgac cacccccgcc aactccacca ccccggcaaa ccccaccggc ggagcggcct
     1681 ggcgcctcca tccacaggcc aataccatca tccttaaatt tgactgaacc cgccgtcagc
     1741 cacgagatcg atccccgtac tatagctgct ctcctccgag gcaaggaaat agatcgccgc
     1801 ggccatctcc tccgcattcc cgatccggcc catcggcacc atctgcgcga actgcgcccg
     1861 gatggcatcc ccctctgcct tggtcttgaa ctgcgaatcg atgatcggcg tctccaccgg
     1921 tcccgggctc aggctgttga cccggatccg ccgctccttg agctcggccg cccagctccg
     1981 cgtgaacgac cgcatcgccg ccttggttgc cgcatacacg ccgtagaacg ggaaaccctt
     2041 aaactgcacc gccgaggata ccagcactac cgaacccccg tcccgcaaaa gcggcagggc
     2101 cttgttggcg ctgaagaata ccccccgcac attgacattg aagatcttgt cgaaatgctc
     2161 aggcgtcgcg gcggccagcg tcgacatctc gacgatcccc gcattgatca ccaggatatc
     2221 gatccccccg cgttcggcct tcaccgttgc atacaaccgg tccaggtccg ccaggctcgc
     2281 gacatccccc tgcaccgcgc tcacattcct gccaatctcc tttttcgcct tttccagctc
     2341 cgccgcacgg cggccggtaa tgaatacata cgccccctct tcgacgagtc tctttgccgc
     2401 agccagcccg atgccgctgc ttccgccggt aatgacggct gtcttgtttt gtagtttgct
     2461 catatgaaaa taattggagg acaaatctaa cattgatata tttacatatc caaatatgtc
     2521 gataaattgg gacatttgtc cccgaataag aacccactga taaagcaaag cagcatggac
     2581 accttcgtca cctatctcag cagcagggcg accttctcgc caaaagagat gcagcagatc
     2641 ctcgccgcat ccaccctcct ccgcttacac cggaaagatc tgctgctgcg acagggtgag
     2701 gtctgccgcg gctacgcctt cgtgaacaag ggctgcttcc ggctctaccg catcggcggc
     2761 gacggcgcgg aacatatcct gcgtttcgcc atcgagaact ggtggatgag cgatatggaa
     2821 agcgtcgatc tccgccaacc ctcgaaatgc acgatcgagg cgctggaaga cagcgaagtc
     2881 ctgttctgga caaaagaaga catgggcgca ctgacgcaaa agatcccggc gctcggcgtt
     2941 gtgtgcgatc agctaaagct ccgcaatcag catgtcaacg agcaacgcat cttcgccgcc
     3001 ctgagctaca ccgccgaaga gaaataccac gaattcatca ccacctatcc cgacctcttc
     3061 aaccgggtgc ccctccatat ggtggcctcc tacctgggca taacaagaaa aaccatctcc
     3121 cgcatccgtg caaaggatgc ccatccccat cggtcctgaa cttttcgcca aaatgctgta
     3181 aatcctccta ttttctgcgt atcttatagg acttgagaac ccttgcgatc atatcgatcc
     3241 tcctgatccc cgccatgggc ctttgccagg aaacaccggc aaaaccaacg ccccaaaaag
     3301 acatcatcga cgtgggcaga tccgtgctga agatccaccc ccgcgtccgg cagaacagtc
     3361 gcaagagtat ctacttctcc atcctcccga catcgagcca ggccgcgggc cccgcaggac
     3421 tatcgatcac cgcaacgacg gcagccttca acctcggcga cgacggcacg ggcttcctct
     3481 ccaccgtcac cttcgcaccc tatgtcgcca cccacggccg catcggcttc gccctgcgct
     3541 ccaatctctg gctgcctcac aacacctggg acatcctcgg ggacagccgc ctgctctact
     3601 atccccagga gacctggggg ctcggcg
//
LOCUS       DATGFG010000267         3778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1177024, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000267 DATGFG010000000
VERSION     DATGFG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177024"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="VKQ52_05000"
     CDS             <1..2085
                     /locus_tag="VKQ52_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLZ86569.1"
                     /translation="CNGRPMKTATAAESLATTGVTVASTFSIAPQSIYVDDKDNLYVA
                     DDKTTVFKWTPAAPPNSRTAVAGGNGAGSAANQLNGVSSIYVDGQDNLFIADAAINHR
                     IQEWPPNARAGTTVINFPNSGELGHFYFYFDCDGNIYSVDILNLAIDEWTPGTGTPSI
                     VARLGAPPPGYVRENISMGKDAAGNLFVAAEDSNNVIKWAPGASTGVVVAGGNGRGAA
                     GDQFNSPLDIKVDGDDTLYIADTENNRVQKWGPGATAGVTVVDGSGLNPPQSQALGVS
                     AICRDKHGSIYVADPKNLQVEKFTMTSLIDSSLSASIPGRYWAVVTDMRGYTHTTDTI
                     LVNTPQAGAPSITVTATATNTPVCVPITFTADTTNAGAAPSFQWEVSGVRVGDGSLQY
                     SNNLFADGDHVYCIMTTQDGCLAGTANDTSNSITLAIDPQGTASVTITAAPPVVCKGD
                     SITFMTQVTNGSAHPTFQWLLNGNPIPGDDSASWHSDSLVTGDVIFCRIISDDVCGLA
                     KSNSIPVVINTPPFIPPGQVFTIAHGKSLTLDPVVNGDIASWRWTPSAGLSDSSIADP
                     VADPASNTLYTLKVTTPAGCSDSAAILVNIYTPLGIPNAFTPNGDGHNDIFYVLGGPY
                     GSRIEDLAIYDRWGQKVFHVHDTSPGDPTTGWNGAVNGRPAPTGTYLYLVVLRTADGL
                     RHQYNGTVVLIR"
     gene            2149..3774
                     /locus_tag="VKQ52_05005"
     CDS             2149..3774
                     /locus_tag="VKQ52_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLZ86570.1"
                     /translation="MRKFPACLAILAGLLCFYAPRGNAQSTYDTVFINKLKEQARASV
                     ATKQKGVQEMVDQIFSFGELGFQEIETSKYLTAFLTKNGFTVEKGIAGIPTAWMARWG
                     SGSPVIALGSDIDCIPKASQKPGVAYKDPIVEGAPGHGEGHNSGQAVIIFAALAVKEL
                     MEKNHLPGTIVIWPGVAEELLGSKAWYIRDGYFNNIDACIFTHVGTEWRCNYGDPGMN
                     GVVSVKFSFDGEAAHAAGAPWRGKSALDAVELMDVGWNFKREHLKPTQRSHYVITDGG
                     DQPNVVPSKAAVWYYFRERNYDDISSMYAAGINIAKGAALMTDTKMSYQILGTAWPGH
                     FNKPIAQAMYENIKQVGYPQWTDADQQLALAVQKLVDAPKTDFEGHPIHGLRSDLDTL
                     KGSVPFSWGGGSDDIADISWNVPTIVLNYPANIPGTKGHHWGDAIAMATPIAHKGTLY
                     GAEAAAMTLVDLFTKPRLLADAKDYFNNIQTKDVKYKPFITKDDPPAINLNSATMEKY
                     REQMRKYYYDPTKYKTYLEQLGITYPTLTPPAK"
BASE COUNT          890 a         1327 c          902 g          659 t
ORIGIN      
        1 tgcaatggcc ggccgatgaa gaccgccacc gcagccgaat ccctcgccac caccggtgtc
       61 accgttgcca gtaccttttc catcgccccc cagtccattt atgtggacga taaagacaac
      121 ctgtatgttg ccgacgacaa aacaaccgtc ttcaaatgga cgccagccgc cccgccgaac
      181 agccggacag cggtggccgg cggcaacgga gcaggctccg ccgccaacca gttgaacggc
      241 gtatcctcca tctatgtcga cgggcaggat aacctcttca ttgccgacgc ggcgataaac
      301 catcgcatcc aggaatggcc gcccaatgca agagccggga ccaccgtaat caacttcccg
      361 aattccggcg agctgggaca tttctacttt tatttcgatt gcgacggcaa catctattca
      421 gtcgacatcc tcaacctcgc catcgacgaa tggacgccgg ggacaggcac gccttccatt
      481 gtcgcccgcc tgggagcgcc gcctcccggc tatgtaagag agaatatatc catgggcaaa
      541 gatgccgcgg gcaatctgtt tgtggcggca gaagatagta acaatgtaat aaagtgggct
      601 ccgggggctt ccacaggggt ggttgtggcc ggcggcaatg gccgcggggc cgcgggcgat
      661 caattcaatt cccccctcga catcaaggtc gacggtgatg acaccctgta tatcgcagat
      721 accgaaaaca accgtgtcca gaaatggggc cccggcgcca ccgcgggcgt caccgtcgtg
      781 gatggctccg gcctcaaccc accccaatcg caggcccttg gggtctccgc catatgccgg
      841 gacaaacacg gcagcatcta tgtcgcggac ccaaagaatc tccaggtgga aaaattcacg
      901 atgacctccc ttatcgactc ctccctttcc gcctcgatcc ccggccgcta ctgggccgtc
      961 gtcaccgaca tgcgcggcta cacccatacc accgatacca tcctggtcaa caccccacag
     1021 gccggcgctc cctccatcac cgtcaccgcc accgcaacaa acacccccgt ctgcgtgccc
     1081 atcaccttca ccgccgatac caccaacgcc ggtgccgccc cctcctttca gtgggaggtc
     1141 agtggcgtaa gagtaggcga cggcagcctc cagtactcca ataacctctt cgccgacggc
     1201 gaccacgtgt attgcatcat gaccacccag gacggctgcc tggccggcac ggcgaacgat
     1261 acctccaata gcatcaccct ggccatcgac ccgcagggca cggcctccgt aacgatcacc
     1321 gccgcaccgc cggtcgtctg caaaggtgac tccatcacct tcatgacgca ggtgactaac
     1381 ggctcggcac atccgacatt tcaatggctg ctcaatggca accccatccc cggcgatgat
     1441 agcgcttcct ggcatagcga cagcctggtc acaggcgacg tcatcttctg ccgcatcatc
     1501 agcgacgacg tctgcggcct cgccaaatcc aacagcatcc ccgttgtcat caacaccccg
     1561 cccttcatcc ctcctggcca ggtgtttacc atcgcccacg gcaaaagcct taccctcgac
     1621 cccgtcgtca acggcgacat tgcctcctgg cgctggacac cctccgccgg gctctccgat
     1681 agcagcatcg ccgaccccgt cgccgacccg gcatccaata ccctttatac gctgaaggtc
     1741 accacccccg ccggctgcag cgacagcgcc gccatcctcg tcaacatcta cacaccgctt
     1801 ggcatcccca atgcctttac gcccaacggc gacggacata acgatatctt ttatgtcctg
     1861 ggaggcccct acggcagtcg catcgaagac ctcgccatct acgaccgctg gggccaaaag
     1921 gtatttcatg tccatgacac atcgcccggt gacccgacaa caggctggaa tggcgccgtc
     1981 aacggaagac ctgccccaac cgggacctat ctgtacctgg tcgtacttag aacggcagat
     2041 ggcctccgcc accagtacaa cggcactgtc gtccttatcc ggtaagcgta ttcatggtag
     2101 tctcgatatc ctgtcgtatc ttggaaagaa ccaaaacgaa tcgatcacat gagaaaattc
     2161 cctgcctgcc tggccatact ggccggattg ctttgttttt atgccccccg cggtaatgcc
     2221 caatccacct acgacaccgt cttcatcaac aaattaaaag aacaggcccg cgcctccgtc
     2281 gccacaaaac aaaaaggcgt ccaggaaatg gtggaccaga tctttagctt tggcgaactc
     2341 ggcttccagg aaatagaaac ctccaaatac ctcaccgcct tcctcacaaa gaacggcttc
     2401 accgtcgaga agggcatcgc cggtatcccc accgcctgga tggcccgctg gggcagcggc
     2461 agccccgtca tcgccctcgg cagcgatatc gactgcatcc caaaagcctc gcaaaaaccc
     2521 ggtgtcgcct ataaggatcc catcgtcgaa ggcgcacccg gccacggcga aggacataac
     2581 tccggccagg cagtgatcat cttcgccgcc ctcgccgtaa aagaactgat ggaaaagaat
     2641 cacctccccg gcaccatcgt catctggccc ggagtagccg aagagctgct gggcagcaag
     2701 gcctggtata tccgtgacgg ttatttcaac aatatcgacg cctgcatctt cacccatgtg
     2761 ggaacggaat ggcgctgtaa ttatggcgat ccgggaatga atggcgtcgt ctccgtaaaa
     2821 ttctccttcg atggggaagc cgcgcatgcc gcaggagccc cctggcgcgg caaaagcgct
     2881 ctcgacgcgg tcgaactgat ggacgtcggc tggaacttca aacgcgaaca cctcaaaccc
     2941 acccaacgtt cccactacgt catcaccgac ggcggcgacc agccaaacgt cgtcccctcg
     3001 aaggctgccg tctggtacta cttccgcgaa cgtaactacg atgacatcag cagcatgtac
     3061 gccgcgggca taaatatcgc caaaggcgcg gcgctcatga ccgacacaaa gatgtcctac
     3121 cagatcctgg gcaccgcctg gcccggccac ttcaacaaac ccatcgccca ggccatgtac
     3181 gaaaatatca aacaggtcgg ctatccccaa tggacagacg ccgatcagca gctggccctc
     3241 gccgtccaaa aactcgtcga tgccccaaag accgatttcg aaggacaccc catccacggc
     3301 ctgcgcagcg acctcgatac gctcaaaggc tccgtcccct tctcctgggg cggcggctcg
     3361 gatgacatcg ccgacatctc ctggaatgtt cccaccatcg ttctcaatta tcccgccaat
     3421 atccccggca caaaaggcca ccactggggc gacgccatcg ctatggccac tcccattgcc
     3481 cacaaaggca ccctctatgg cgcggaagcc gcggccatga ccctggtcga cctgttcacg
     3541 aaaccccggc tcctcgccga cgcaaaagac tatttcaaca acatccagac aaaagacgtc
     3601 aaatacaaac ccttcatcac aaaggacgac ccgcccgcca tcaatctgaa cagcgcgacc
     3661 atggaaaaat accgcgaaca aatgcggaaa tactattacg accccacaaa atacaagacc
     3721 tacctggagc agctgggtat cacctaccct accctcaccc caccggcaaa ataactac
//
LOCUS       DATGFG010000268         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1053092, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000268 DATGFG010000000
VERSION     DATGFG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053092"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VKQ52_05010"
     CDS             complement(<1..485)
                     /locus_tag="VKQ52_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86571.1"
                     /translation="MKRTIYLLHRRLSIIMAIPVLLWASSGMMHPVMTNIRPAVATQV
                     FVPAPVDLGRIRMPLDSALRRRHLDSFSAVRLVQIDTNWFWQVRPAGAGAVPVYLSCK
                     NGNVLTAGDWLYAQWLARYFLEGGAALRGVGGAGSRGGMVMTGSGGVVEAASRGAAMR
                     GS"
     gene            complement(487..2589)
                     /locus_tag="VKQ52_05015"
     CDS             complement(487..2589)
                     /locus_tag="VKQ52_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86572.1"
                     /translation="MNVFFRYGVVAMAWVCPILLFGQGPDSPHVSRLDELVVTASRTA
                     QRRTEAPVAISTVSARTIAETKANQLDQLLNKVSGVFMVDLGNEQHEMSIRQPMSTAG
                     VFLYLEDGIPIRTTGIYNHNALLEMNAAAAGQIEIIRGPASSLYGAEAIGGAVNVITQ
                     APPALPKGYVSVQRNNNGYTRADAQVGTAFGKWGVIASGYYANRTDGPIQYSDFHKTA
                     VTIRADGRLSDRLVWTNALTYVNYYSDMYGSLDSVHFAQRNYHTPYSFTYRKVESLRA
                     RSTLDWRWNDRAATQVTVVFRNNSIGQDPSYYISNSRSNPYLANGQINDNSFHSWMAL
                     VQHRQDFRFLGSRLIAGFSADFSPSTYWAKYIAIQRDAKGNYGNYTPTDSLLSNYATG
                     ISNIAEYLNYEVSPFHGLKVVAALRYDLFHYTFRNYLPPSALTGSPDTKNDFNRVTPK
                     VGVTYNYKGIGFYANYSQGYVPPQVTDLYNGVKVPYLRPQTFFNYEAGGWLALLQNKL
                     YVDWSVYRMDGTNQIISVRQNDGTFINENAGKTRHEGVEYGVTVRPSEEWTLRVSGTQ
                     ADHWFVVQYENGQNYNGRQMAAAPHFIANAELTWRPHFLKGFRIGAEWQRVGRYWMDD
                     ANTREYAGFGIVHFRTGYSFRAWEVWVNALNAFNRYYAALATKSTYGYSYNLGDPAEV
                     TVGFAWHPWSTSRSGHQK"
BASE COUNT          622 a          990 c          668 g          557 t
ORIGIN      
        1 gagcccctca tcgccgcacc ccggctcgcc gcttccacta ctccacccga tcccgtcatc
       61 accattcctc cccggctccc ggcgccccct actccacgca aagccgcacc cccttctaaa
      121 aaatacctcg ccaaccactg cgcatacaac caatctcccg ccgtcaacac attcccattt
      181 ttacagctca aatacaccgg caccgcgcca gcccctgccg gccgcacctg ccagaaccag
      241 ttcgtatcga tctgcaccag ccgtaccgcc gaaaaactat ccaggtgccg ccgccgcaac
      301 gccgaatcca acggcatccg tatccggccg agatccacgg gcgcaggcac aaacacctgc
      361 gtcgccaccg ccggccggat attggtcatc accgggtgca tcattccact cgaagcccaa
      421 agcaacacgg gtatcgccat gatgatcgac agccgccggt gtaacaggta aatagttcgc
      481 ttcatatcat ttttgatgtc cggaacgcga cgtgctccag ggatgccagg caaaccccac
      541 cgtcacttcg gccgggtcgc ccaaattata actataccca tacgtcgact tcgtcgccaa
      601 cgccgcataa taccggttga acgcattcaa cgcgtttacc cagacctccc aggcccggaa
      661 actatatcct gtccggaaat gcacgatccc aaaacccgca tactcccgcg tattcgcatc
      721 gtccatccaa taccgcccca cccgctgcca ctcggcgccg atccggaacc ccttcaggaa
      781 atgcggccgc caggtcagct cggcattggc gataaaatgc ggcgccgcgg ccatctgccg
      841 cccgttgtaa ttctggccgt tctcatattg cacgacaaac cagtgatccg cctgcgtacc
      901 actcacccgc aacgtccact cctccgaagg ccgcaccgtc accccatact cgacgccttc
      961 atgcctcgtc ttccccgcat tctcgttgat gaacgtgccg tcattctgcc gcacggaaat
     1021 gatctggttc gtcccatcca tccggtacac actccagtcc acatatagct tgttctgcaa
     1081 aagcgccagc catccccccg cctcatagtt gaaaaaggtc tgcggccgga gataaggaac
     1141 ctttacccca ttgtacagat cggtcacctg cggcggtaca tacccctggc tgtaattggc
     1201 gtaaaacccg atccccttgt aattataggt caccccgacc ttcggcgtca cccggttgaa
     1261 atcattcttc gtatccggcg accccgtcaa cgccgacggc ggcagatagt tccggaaagt
     1321 ataatggaag agatcatagc gcaatgccgc caccaccttc aacccatgaa aggggctcac
     1381 ctcgtaattc aggtactccg cgatattgct gatccccgtc gcatagttgc tcagcagcga
     1441 atcggtcggg gtataattcc cataattgcc tttcgcatcc cgctggatag cgatatactt
     1501 cgcccaatac gtgctcgggc tgaaatccgc gctaaacccg gcgatcagcc ggctcccaag
     1561 gaaccggaag tcctgccggt gctgcaccag cgccatccag ctgtgaaagc tgttgtcgtt
     1621 gatctgcccg ttggccagat agggattgct gcggctgttg ctgatatagt aggaagggtc
     1681 ctgcccgata ctgttattcc ggaaaacgac cgtcacctgc gtggccgccc ggtcattcca
     1741 ccgccagtcc agcgtcgatc gcgcccgcaa cgattccacc ttccgatagg tgaaggagta
     1801 gggcgtatga tagttccgct gcgcaaaatg cacactgtcc agcgacccat acatatcgct
     1861 gtagtaattc acatacgtca gcgcattcgt ccaaacaagc cggtcgctaa gccgcccatc
     1921 ggccctgatg gtcaccgccg tcttgtgaaa atcactgtac tggatgggac catccgtgcg
     1981 gttggcataa tagccgctgg cgatgacccc ccatttccca aaagccgtcc ccacctgcgc
     2041 atcggcccgt gtatacccgt tgttattccg ctgcacactc acatacccct tcggcaaggc
     2101 cggcggcgcc tgcgtgatca cattcaccgc cccaccgatg gcctcggcgc catacagcga
     2161 ggaagccggc ccccggatga tctcgatctg cccggccgca gccgcattca tctccagtaa
     2221 agcattgtga ttatagatgc ccgtggtccg gatcgggata ccgtcctcca ggtaaagaaa
     2281 aaccccggcc gtgctcatcg gctggcggat gctcatctcg tgctgctcat tgccgagatc
     2341 gaccataaaa accccgctca ccttgttgag cagctgatcc agttgattgg ccttcgtctc
     2401 ggcgatagtc cgcgcactca cggtactgat ggctacaggc gcctccgtcc tccgctgtgc
     2461 cgtccggctc gcggtaacga ccagctcatc gagcctgctg acatgcggcg agtccgggcc
     2521 ctgcccaaac aatagtatcg ggcataccca tgccatggcc acaacgccat agcgaaaaaa
     2581 tacattcatg gaaagttggt tgaagttgga aatggccggc cccttcgggc gaccggtaac
     2641 acatactaca aagaaggaaa gcgcctatcc ccttcggggc ggagcaaaaa aggaaggaag
     2701 atactcctgc gcctcgcgat cgatatgccg cgcatgacgc tcaaccgtca ccatcactac
     2761 cgccggctcc ggcaattcat tgccgggacg atgcacctgc aacaacacct cctcgtgctg
     2821 cgcaccctta cccggcg
//
LOCUS       DATGFG010000269         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_315070, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000269 DATGFG010000000
VERSION     DATGFG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315070"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VKQ52_05020"
     CDS             <1..806
                     /locus_tag="VKQ52_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HLZ86573.1"
                     /translation="DYSAPKVDVWGISDKNLFLEANKVLKKEQGPFFAVIQTADNHRP
                     YTIPAEDRQAFQPVTASPEELRRNGFESNEEMNAFRYTDFGYRTFIEAASKEAYFDNT
                     IFLFVGDHGIPGDAAALFPRAWTDQRLTAEHVPLLIYAPKLLRPRRVSRECSQLDVLP
                     TLAGLCHLSYRNTTLGRDILDTARYAGREMAFIYDPDQFYIGVVKGDYYYRRQLKTGK
                     EEMVSVVGNDPPPAGVLQGQVKQQMAQLSEGIYEAARYMLLMNKKKQGR"
     gene            complement(818..1600)
                     /locus_tag="VKQ52_05025"
     CDS             complement(818..1600)
                     /locus_tag="VKQ52_05025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ86574.1"
                     /translation="MAIASAENSPVNSNIPRDWFRDWFDSPYYHKLYFQHNEQEAAAF
                     INRLLALLQPPAAADMLDVACGRGRHARMLAAHGYDVTGIDLAPGSIDYARRFETDRL
                     HFYIHDMRQLLCVNCFDYAFNFFTSFGYFRTRRENINTIRMVSIALRPKGVFVLDYMN
                     APYVKAHLVPNEQKIVDGTTYTITRWCDGKHFYKKIVIEEEKLPAPLEYTERVAVLTP
                     EDLGALLEPNGLLIRGRYGNYQLEPFDPGQSPRLLLIAEKQA"
     gene            1687..>2667
                     /locus_tag="VKQ52_05030"
     CDS             1687..>2667
                     /locus_tag="VKQ52_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86575.1"
                     /translation="MALIKSISGIRGTIGGKPGENLSPLDIVKFTAAFGSRILQINKG
                     TKIVIGRDGRVSGEMVSALVVNTLLGLGLDVLDLGLSTTPTVELAVPGEKAAGGIILT
                     ASHNPQEWNALKLLNSTGEFISAEEGKAVLDKAQKEDFVFATVDKLGSYTQSDAWLQK
                     HIDAVVKYPLVDVAAIRQKKFRIVVDTINSTGALFIPPLLKALGVAEVILINEEINGK
                     FAHNPEPLPENLAGLSAKVQKSKADLGIATDPDVDRLCFVNEDGSMFGEEYTLVAVAD
                     YVLSKRPGNTVSNMSSTKALKEITLSRGGEYHPSAVGEVNVVNAMKAVNAV"
BASE COUNT          582 a          624 c          867 g          594 t
ORIGIN      
        1 aggattacag tgcaccaaag gtggatgtct ggggcatcag cgataagaac ctgttcctgg
       61 aggccaataa ggtattaaag aaggagcagg ggcccttttt tgcggtgatc cagacggcgg
      121 ataaccatcg cccttatacg atcccggcag aagaccggca ggcttttcaa ccggtgacgg
      181 cgagccctga agagctccgg cgcaatggtt tcgagagcaa cgaggagatg aatgccttcc
      241 ggtataccga ttttggctac cggaccttta tcgaagccgc gtcgaaggag gcttatttcg
      301 acaatacgat attccttttt gtcggggatc atgggatccc gggggatgcc gccgcccttt
      361 ttcccagggc ctggaccgac cagcggctga cggccgagca tgtcccgttg ctgatctatg
      421 cgccaaagct gctgcggccg agacgggtga gccgggagtg ttcccaactg gatgtgttgc
      481 cgacgctggc cgggctttgt catctgtctt accggaacac caccctgggc agggatatcc
      541 tggacacggc ccggtatgcg gggagagaga tggcgtttat ctacgatccc gaccagttct
      601 atatcggcgt ggtgaagggg gattattatt atcgccggca gttgaagacg ggcaaggagg
      661 agatggtgtc ggtggtcggg aatgatccgc cgccggccgg tgtgctgcag gggcaggtga
      721 agcagcagat ggcgcagttg tcggaaggaa tatatgaggc ggcgcggtat atgctgctga
      781 tgaataagaa aaaacaaggc aggtagatcg cgtatgatca tgcctgtttc tccgcgatca
      841 gcaggaggcg cggggactgg ccgggatcga agggctccaa ttgataatta ccgtatcgtc
      901 cgcggatgag cagcccattg ggctccagca gcgccccgag atcttccggt gtaagcacgg
      961 ccacccgttc ggtgtattcc agcggggccg gcagtttttc ttcttcgatc acgatctttt
     1021 tatagaaatg ttttccgtca caccaccggg tgatggtata ggtggtgccg tcgacgatct
     1081 tttgttcgtt cgggacgaga tgcgccttca cgtaaggggc attcatgtaa tcgagtacga
     1141 agacgccttt tggccgcagg gcgatgctga ccatacggat cgtattgata ttctccctgc
     1201 gggtgcggaa atagccgaag ctggtgaaga aattgaaggc ataatcaaag caattgacgc
     1261 agaggagctg gcgcatgtca tggatgtaga aatgaagacg gtcggtttcg aaccggcggg
     1321 catagtcgat gctgccgggt gcgagatcga tgccggtgac atcgtatccg tgggctgcca
     1381 gcatccgggc gtgccggccc cggccacagg cgacatcgag catgtcggcg gcggcgggtg
     1441 gctgtaataa agccaggagc cggttgataa aggcggccgc ttcctgttcg ttgtgctgga
     1501 aatagagttt gtggtagtag ggggagtcga accaatccct gaaccagtcg cggggtatat
     1561 tgctgtttac gggggagttt tctgccgatg ctatggccat gggtcctttg cttttggtaa
     1621 aattaggtca aatccctttc ggcggattta caggaaacct tatttttgct gataaatcta
     1681 agtgctgtgg cattgatcaa aagcatctcc ggtatcaggg gtacgatcgg ggggaagccg
     1741 ggcgaaaacc tctctccgct ggacattgtt aagtttaccg ctgcttttgg cagtagaatc
     1801 ctgcaaatca acaagggaac gaagatcgtg atcggccgtg acggccgtgt cagcggggaa
     1861 atggtcagcg ccctggtggt taatacgctg ttgggattgg gactggatgt gcttgatctt
     1921 gggctgtcca cgacgcccac ggtggagttg gctgtgcccg gtgagaaggc ggcggggggg
     1981 atcatcctga cggccagtca caatccccag gaatggaatg ccctgaaatt gctgaacagc
     2041 accggtgagt tcatcagtgc cgaggaagga aaggcggtgc tggataaggc ccagaaagaa
     2101 gattttgttt ttgccacggt ggataagctg ggcagttata cgcaatcgga tgcctggttg
     2161 caaaagcata tcgatgccgt tgtcaaatat ccccttgtgg atgttgccgc cattcgccag
     2221 aagaagttca ggatcgtggt cgatacgatc aactccaccg gcgccctgtt cattccaccc
     2281 ttgctcaagg cgctcggggt ggcggaggtg atcctgataa acgaagagat caatggaaag
     2341 ttcgcgcata accctgagcc gctgccggag aacctggccg ggctcagcgc caaggtccag
     2401 aagtcgaagg ccgatcttgg catcgccacg gacccggatg tggaccggct ttgttttgtc
     2461 aacgaggatg ggagtatgtt cggggaggag tatacccttg tggcggtggc cgattatgta
     2521 ctgagtaagc gtcccggcaa tacggtgagc aatatgagca gtacgaaggc ccttaaggag
     2581 attacactca gccggggagg ggaataccat ccttcggcgg tgggtgaggt caatgtggtc
     2641 aatgcgatga aggcggtgaa tgcggtg
//
LOCUS       DATGFG010000270         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1039059, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000270 DATGFG010000000
VERSION     DATGFG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039059"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VKQ52_05035"
     CDS             <1..287
                     /locus_tag="VKQ52_05035"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="RNase BN; member of metallo-beta-lactamase family;
                     the purified enzyme from Escherichia coli forms dimeric
                     zinc phosphodiesterase; in Bacillus subtilis this protein
                     is a 3'-tRNA processing endoribonuclease and is essential
                     while in Escherichia coli it is not; associates with two
                     zinc ions; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HLZ86576.1"
                     /translation="ADTLYDERIAEQVKGVDLLYHETTYLKDLADQAGKRFHSTTAQA
                     ATIARIAGVHRLLIGHFSSKYEKLQLFEQEAKEIFPNTDLAMEGVTYRVL"
     gene            complement(288..1760)
                     /locus_tag="VKQ52_05040"
     CDS             complement(288..1760)
                     /locus_tag="VKQ52_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86577.1"
                     /translation="MRKTVIRGGVRKTATWSGVSKTVIWSGGVLVVLLITAYFIADVL
                     VRKKVDEALQALPPSLHVTYHSLHSHLFNSSLEIGGVDIRFSRDKHVYEATIGQVAAE
                     GIHFFELMRTHRLRLRSLRLDGIEASGEEMRLSLAGSAEFDSVYASDKGEKGFGAMRL
                     SLRKLRCQLPATREMLHLSNMVLDSRKRVFSLDTLRVMPSVDKSEIGRERGYQVDVVE
                     ATSEGIEMQGLDVMGLPEHRVVADRLTVRRNHLYVFRDRRLPLEPGIKSLPMESLMEL
                     PVSLRIGTVDIGTTDFTYEEFPKDGEKSGTLSIYRMKARLHPLINRPEAGDPAYIALT
                     TEGSLMNSGIVTATTKMPLHKHEPYLVEGAFHDLDVTRLNNPAEHLGQLHLESGMLNS
                     LAFWFEMGDEKATGKIVGEYHDLVVDKLKANGKVAKLKSFALKKLIIPRNKDKSLPEK
                     KRTGKVDYRRDPQRYFSYYLLHALLVGVKSSFSLGFLLPG"
     gene            complement(1802..>2622)
                     /gene="dapA"
                     /locus_tag="VKQ52_05045"
     CDS             complement(1802..>2622)
                     /gene="dapA"
                     /locus_tag="VKQ52_05045"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HLZ86578.1"
                     /translation="LSIDFTALATIIDFVIDGGVEYIVTMGTTGETPTLSKEEKKDII
                     QFTYEKVAGRVPVVVGVGGNNTAELEKELSWFPLDKAVAVLSASPYYSKPSQEGLFQH
                     YKALAGASPKPILLYNVPGRTGRNLSASTTIRLALEVKNIAGIKEASGDMAQCMEILR
                     DRPREFLVVSGDDALALPQIACGMDGVISVAANSFPKAFTEMVRLSLRGDFREAKSLN
                     DRLIAGYDLLFAENNPAGVKAAMYELKLIGNYLRLPVVPVSEGLQAKIREYVIG"
BASE COUNT          545 a          815 c          695 g          567 t
ORIGIN      
        1 ccgccgacac gctctatgac gaacgcatcg ccgaacaggt aaagggggtt gacctgctat
       61 atcacgagac cacctacctg aaagacctgg ccgaccaggc gggtaaacgc ttccacagca
      121 ccacggcaca ggcagcgacc atcgcccgca tcgcgggagt tcaccggctg ctcatcggtc
      181 atttcagctc caaatacgaa aaactgcagc tcttcgaaca ggaggcaaag gagatcttcc
      241 ccaataccga cctggcgatg gaaggggtaa cctacagggt tttgtaacta ccccggcagc
      301 aggaatccca gcgaaaaact cgacttcacg cccaccagca acgcatgtaa caggtaatag
      361 gaaaaataac gctgcgggtc ccgccggtaa tccaccttgc cggtccgctt cttctcgggc
      421 agcgacttat ccttgttcct gggaattatg agcttcttca gggcaaatga cttcagtttg
      481 gccactttcc cgttcgcctt caacttatcc accaccaggt cgtggtactc gccgacgatc
      541 tttcccgtag ccttctcgtc acccatctcg aaccaaaacg caaggctgtt cagcatcccc
      601 gattccaggt gaagctggcc gagatgttcc gccggattgt tcagccgggt cacatccagg
      661 tcgtgaaaag ccccttccac gagatacggt tcatgcttat gcaggggcat cttcgtcgtt
      721 gccgtgacga tcccggaatt catcagcgat ccttccgtgg tcagggcaat ataggcggga
      781 tcaccggctt ccggccggtt gatcagggga tggagcctcg ctttcatccg gtagatgctg
      841 agcgttccgc tcttttcgcc gtcttttggg aactcttcat acgtaaagtc cgtcgtcccg
      901 atgtcgaccg ttcctattcg cagcgacacg ggcagctcca ttaaagactc cattggcagg
      961 gatttgatac cgggttccag gggaagccgc cggtcacgga aaacgtagag gtggttgcgg
     1021 cgcaccgtga gccggtccgc aacgacccga tgctccggca aacccattac atcgaggcct
     1081 tgcatctcga taccttcgct ggtcgcctcc acgacatcca cctggtatcc cctttcccgg
     1141 cctatctccg acttatctac cgacggcatc acccgcaggg tatccaggct gaacacccgc
     1201 ttcctgctgt ccaaaaccat attgctcagg tggagcatct ccctggtggc cggtagctgg
     1261 cagcgcagct tacgcagcga caaccgcatc gctccaaacc ctttctctcc cttgtccgat
     1321 gcgtacacgg aatcaaactc cgcactccct gccaacgata gccgcatttc ttcgccgctc
     1381 gcctcgatcc catccaggcg caggctccgc aatcgcagcc ggtgtgtccg catcagctcg
     1441 aaaaaatgga tgccttccgc ggccacctgc ccgatggttg cttcatagac atgcttgtcc
     1501 cgggaaaaac ggatatccac gccgccgatc tccagcgaac tgttgaacaa atgggaatgc
     1561 agggaatgat aggtcacgtg gagggacggt ggcagggcct gtaatgcctc atccactttt
     1621 ttgcggacca gaacatccgc gatgaaatag gcggtgatca gcaagaccac cagcacaccc
     1681 ccgctccaga tgactgtctt actcaccccg ctccaggtgg ctgtctttct caccccgccc
     1741 cggatgactg tcttcctcat aagaaaagga aacagcaagc tccatgcccg cggttgcctt
     1801 cttaacctat gacgtattcc ctgatctttg cctggagccc ttcgctcacc ggcacaaccg
     1861 gcaggcgcag gtagttcccg atcagtttca gctcatacat cgcggctttt accccggcgg
     1921 ggttgttctc ggcaaaaaga aggtcatacc ctgcgatcag gcgatcattg agcgatttgg
     1981 cctcccggaa gtcgcctctg agggaaagcc ggaccatttc cgtgaaagcc ttggggaagc
     2041 tgttggccgc gacactgatc accccgtcca tgccacaggc aatctgcggg agagcgaggg
     2101 cgtcgtcccc gcttaccacg aggaattccc tgggccggtc gcgaaggatc tccatgcact
     2161 gggccatgtc tccgctggcc tctttgatcc cggcgatatt ttttacttcc agggcaagcc
     2221 ggatggtagt ggacgcgctc agattccggc cggtccggcc gggaacatta tagaggagga
     2281 tgggtttcgg cgaggcgccc gcaagggctt tatagtgctg gaaaagccct tcctgggagg
     2341 gtttgctgta gtagggactg gcgctcagca cggccacagc cttgtccagc gggaaccagg
     2401 acagctcttt ttccagttcg gcggtgttgt tgccgccgac acccacgacg acgggcacgc
     2461 ggccggccac cttttcatag gtgaactgga tgatatcttt tttctcttct ttggacaggg
     2521 tcggggtttc acccgtggtg cccatggtta cgatatattc cacgccaccg tcgatcacaa
     2581 agtcgatgat cgttgccagg gcggtaaagt cgatgctgag at
//
LOCUS       DATGFG010000271         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_389265, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000271 DATGFG010000000
VERSION     DATGFG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389265"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VKQ52_05050"
     CDS             <1..836
                     /locus_tag="VKQ52_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLZ86579.1"
                     /translation="ILGERVKMTIAFNTILIPVDFSQNTEIAVKKAAELGGRDGIELH
                     LLHVVKAGADVEAAFEQLEEMIRVKYPAVRVRTHVLQGRSVQRVIIECARLLQPQLIV
                     IGKSDPPRRWAFFKGVSPDVIAKRSDCPVLTVKPGSADSKTRVILIPIGNFLPERKLE
                     WAVALAKKYRAQIHLLAIQDRQESKEWALPQVFLKAYNQLREHLHHPIEYSATGKQGT
                     AKAALNCAEMIMADMIVVNPETESGVSGFAGYRHISDLLGKDSKIQVLDIEPYKNENQ
                     N"
     gene            843..2504
                     /locus_tag="VKQ52_05055"
     CDS             843..2504
                     /locus_tag="VKQ52_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ86580.1"
                     /translation="MKKMFFVVFIFFMMGGSRAQSIAEARKLVYYERYAGAAHQLQSV
                     LHNDPANSEAWLLLTQVYIHQKRIPALRDTLSRMPAAAGQQPLGLCAYGQLLLQEHRK
                     DSALGYFNRALEMTKEKDVPVLMAIARAQDVADSSDANYAYAVGLLSKAIKRDKRDAG
                     LYVELGNIYRRLMDGTNSYKAYQDALAQDDRNVEALYRLGKIFVSQNNAEMYLKYLNE
                     AVAADSLYAPAWFELYYHYYFRDVNKAMECLDHYIAGSDASVGNDYLVTELLYSSRKY
                     SAAIEHAQRLIGQQGKVTEPRLYKLVAYSYKELHDSTKALDYMKQYFREQADTGFVVK
                     DYSTMGEIYASLGQPDSAVGYFVKAGNMEKDSALRSEYARKLAGLYKQEKDYSNQALW
                     LGRYYAGNTKATNLDLFNWGLAHYMAREYPAADSVFGLYETKYPEQDFGYYWRARCDA
                     AMDTAMANGTAIPHYLKLIDIDSKDSANRTNRKHLIEAYGYIAAYKANTEKDFAGSID
                     FFDRLLALDPGNTDAQRYVGILKKNMAKAAAKSETVKGEATKADK"
     gene            2625..>3602
                     /locus_tag="VKQ52_05060"
     CDS             2625..>3602
                     /locus_tag="VKQ52_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86581.1"
                     /translation="MEQRRHNPTRWREYELILITIFCLFGIAGSWWKIFEYRGAELRD
                     RWGRQFAENHVDFDFFINVLLPETGLLVMIYIAYFWMNLYILPRLVQVEAAEPGRFRI
                     NFSLRGHIDFSGPGGETLKRFLWGLANAIALILLLGIGWGVVIYYRREYEFSGMDWTS
                     LANRVLGLGWKNSAGLVVLYTGYAFIRELSIRKLLSDPKRNAFRIELVNQISTYVLVY
                     FTVGFVLYFFNIVDDDVFFIVFWAIAPVVLLSAITNIYWIFPLVGEKGFFRRPILRRI
                     LLSTLAWSAIVPFFLIPDSPSMVEAVLSLWCGQLFIGTPLSWVIFRLRKD"
BASE COUNT          773 a          819 c         1167 g          843 t
ORIGIN      
        1 ttattttggg agaaagagta aaaatgacga tcgcattcaa taccatcctg atccctgtgg
       61 atttttccca gaataccgaa atagcggtga agaaggcggc ggagctgggc ggcagggacg
      121 ggatcgagct gcacctgctg catgtagtga aggcaggcgc ggatgtggag gcagccttcg
      181 aacagctgga ggagatgatc cgggtgaagt acccggccgt gcgggtgcgg acgcatgtgc
      241 tgcaggggcg ttcggtgcag cgggtgatca ttgaatgcgc gaggttgctg cagccgcaac
      301 tgatcgtcat cggcaagagc gacccgccgc ggagatgggc ctttttcaag ggtgtctcac
      361 cggatgtcat tgccaaaagg agtgattgcc ccgtgctgac cgtcaagccg ggatcggccg
      421 acagcaagac gcgggtgatc ctgatcccga ttggtaattt cctccccgag cggaagctgg
      481 aatgggcggt agcgctggcg aagaaatacc gggcccagat acatctcctt gccatccagg
      541 accggcagga gtccaaggag tgggccctgc cgcaggtgtt cctgaaagcc tataaccaat
      601 tgcgggagca tctccatcac ccgatagaat actcggccac cggaaagcag ggcacggcga
      661 aggcggcgtt aaactgcgcc gagatgatca tggcggatat gatcgtcgtc aatcccgaga
      721 cggagagcgg ggtgtcgggt tttgcgggct accggcatat cagtgacctg cttgggaagg
      781 attctaagat acaggtgctg gacatagagc cttataagaa cgaaaaccaa aactgagtgt
      841 gtatgaaaaa aatgtttttt gttgttttta tcttctttat gatgggtggc tccagggccc
      901 agtcgatagc ggaggcgcga aagttggtgt attatgaacg ctacgccggg gccgcccatc
      961 aattgcagtc ggtgttgcac aatgatccgg ccaattcgga ggcgtggttg ttattgacgc
     1021 aggtgtacat ccaccaaaag cggataccgg cgttgcggga tacgctttcc cggatgccgg
     1081 ccgcggcggg gcagcagccg ctggggcttt gcgcctatgg gcagctgttg ttacaggaac
     1141 acaggaagga cagtgcgctg ggctatttca accgggcgct ggagatgacg aaggaaaagg
     1201 atgtgccggt attgatggcg atagcccggg cgcaggatgt ggccgattcc agcgatgcga
     1261 attatgccta tgccgtcggg ctgttgagca aggcgataaa aagggataaa cgggacgccg
     1321 ggttgtatgt cgagctcggg aatatctatc gccggctgat ggatggcacg aactcctaca
     1381 aggcctacca ggatgcgctg gcgcaggacg atcgcaacgt ggaagccttg tacaggctgg
     1441 ggaagatctt tgtctcacag aacaatgcgg agatgtacct gaagtatctc aacgaggcgg
     1501 ttgccgccga ttcgctgtat gcgcccgcct ggttcgagtt gtattaccat tattatttcc
     1561 gggatgtcaa taaggcgatg gagtgcctgg accattatat cgccgggtcc gatgcgagtg
     1621 tcgggaatga ttacctggtc accgagttgc tctattcctc gcgaaagtat tcggcggcta
     1681 tcgagcatgc gcagcggctg atcgggcagc aggggaaggt gacggagccg aggctttaca
     1741 aattggtcgc ctattcttac aaggagctgc atgacagtac gaaggcgctc gattatatga
     1801 agcagtattt ccgggagcag gcggacaccg gctttgttgt caaggattac agcacgatgg
     1861 gggagatcta tgcttcgttg gggcagccgg acagtgcggt gggctatttt gtgaaagcgg
     1921 gaaatatgga gaaggacagt gccctgcggt cggagtatgc gcggaaactg gcggggctgt
     1981 ataagcagga gaaggactat tccaaccagg ctttgtggct tgggcggtat tatgcgggga
     2041 atacgaaagc cacgaatctc gatcttttca actggggcct ggcgcattat atggccaggg
     2101 aatatcctgc cgcggacagt gtttttggcc tctatgagac aaaatatccc gagcaggatt
     2161 ttgggtatta ctggcgggcg cgttgtgatg cggcgatgga tacggctatg gccaacggga
     2221 cggctatccc gcattatctg aaattgatcg atatcgacag taaggattcc gccaacagga
     2281 cgaaccgcaa acatcttatc gaagcgtatg ggtatatcgc cgcctataag gccaatacgg
     2341 agaaggattt cgcggggtcg atcgatttct tcgaccggct gctggcgctg gacccgggga
     2401 ataccgatgc ccagcggtat gttggtatcc tgaaaaagaa catggccaag gcggcggcga
     2461 agagcgagac ggtgaagggg gaagcaacga aagcggacaa gtagatggcc gatgggccga
     2521 ggacgatcct gccggcggcc aggcttccat cacattttcg ccgtgtttta tcacatttgg
     2581 cggggatgcc tgtggttttg ctttaatctt gtcgaaaaaa caagatggag caacgcaggc
     2641 ataatccaac gagatggcgg gagtatgaac tgatcctgat cacgatcttt tgcttattcg
     2701 gtattgcggg gtcgtggtgg aagatcttcg agtatagggg cgccgagctt cgggaccggt
     2761 ggggacggca atttgccgag aatcatgtgg atttcgattt tttcatcaat gtgttgttgc
     2821 cggagacggg tttgctggtg atgatctata tcgcgtattt ctggatgaac ctgtatatcc
     2881 tgccgcggct ggtgcaggtg gaggccgccg aaccgggacg gttccgcatc aatttctcgt
     2941 tgcggggcca tatcgatttt agcgggccgg gcggggagac gctgaaacga ttcctttggg
     3001 gcctggctaa tgcgatagcg ctgatccttt tgctggggat cggctggggg gtggtgatct
     3061 attaccggcg ggaatacgag ttctcgggga tggactggac gagcctggcc aaccgggtac
     3121 tgggccttgg ctggaagaat tcggcggggc tggtggtatt gtacaccggc tatgcgttca
     3181 tccgggagct gagcatccgg aagctgcttt ccgatcccaa acggaatgct ttccggatcg
     3241 agcttgtcaa ccagatcagt acttatgtac tggtttattt cacggtgggt tttgtgttgt
     3301 attttttcaa catcgtcgac gatgacgtgt tctttattgt tttttgggcg atcgcccctg
     3361 tggtactgct ctcggcgata acgaacatct attggatatt cccgcttgtc ggtgagaagg
     3421 ggtttttccg gcggcccatc cttcggcgaa ttttgttatc cactcttgcg tggagcgcca
     3481 tcgtgccttt ctttctgatc ccggacagcc cgtcgatggt ggaggcggta ttgtctttgt
     3541 ggtgtgggca attgttcatc ggcactccct tatcctgggt tattttccgg ttgcggaagg
     3601 ac
//
LOCUS       DATGFG010000272         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_668836, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000272 DATGFG010000000
VERSION     DATGFG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668836"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VKQ52_05065"
     CDS             complement(<1..255)
                     /locus_tag="VKQ52_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86582.1"
                     /translation="MKKLFFMGLFLPALALLSFYPFSTGEGSLPIGATLPKSDILMKD
                     VSGKSISLRDVKRENGLLVMFSCNTCPYVIRNQGRTKEVCT"
     gene            complement(281..1225)
                     /locus_tag="VKQ52_05070"
     CDS             complement(281..1225)
                     /locus_tag="VKQ52_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLZ86583.1"
                     /translation="MIKLERKLYDSAPFRFLEARSKKVTLPGFQGRALFDVIHFFLSQ
                     VKKVGLIDRARSIAFSFLTAIPAATIFICTLVPYLPVSRQIERQLLLLTKDITPNQNT
                     YVLVSRFLEDFLDKPRVGLLSFGFVLALFYSSNAMIGIMRSFNKSLIYNTRRNALQTR
                     WMAIKLTTMVLCMIIGSVIILVTQAELLRSILKALHISNNDHSLRWLFRTLRWVVIIP
                     LFYFAIACIYKYGPAVKERWSLFSPGTLLATFLTIMTTMAFSYWVNRFGTYNKVYGSI
                     GTVMILMILVYFNSLVLLIGYELNVSIHHVKAMAEEKR"
     gene            complement(1232..2290)
                     /gene="mltG"
                     /locus_tag="VKQ52_05075"
     CDS             complement(1232..2290)
                     /gene="mltG"
                     /locus_tag="VKQ52_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HLZ86584.1"
                     /translation="MKKFILGSVLLLLVAGCFVGWRVFGSATAFSGDNYYLYIHTGMT
                     YEQVLDTLKKDEVIQSPAIFNWVAGRMGYPRSVRAGKYEIQKGMSIVGILRMLHNGRQ
                     APVKLVITKFRTLEGLAGAIGKKLECDSAALGSYLHNNDSLHAFGVDSNTLLAIVVPN
                     TYTSFWNTTPSALFRKMYSTYKAWWTPARVEAARARGLTPVTATILASIVEEETNAQA
                     DKGKIASVYLNRMARGIRLSADPTIKYALRDFDLRRIYDKYLLVESPYNTYQHEGLPP
                     GPICTPSEPTLEAVLASPTTDYLYFVAKPDFSGYSNFAATYKEHLEYAKAYRDALDKQ
                     QGIKAGADSLKKPEGRKK"
     gene            complement(2387..2707)
                     /gene="secG"
                     /locus_tag="VKQ52_05080"
     CDS             complement(2387..2707)
                     /gene="secG"
                     /locus_tag="VKQ52_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HLZ86585.1"
                     /translation="MIFFVILIILACGVLGLIILVQNPKGGGLAGNVGGFSNQLMGVK
                     QTTDVLEKGTWALAIVVGLLCLFSAVFVPRASGSSQRVPEKVNATAPAGNPTAPAGTA
                     PAQK"
     gene            complement(2709..>3566)
                     /locus_tag="VKQ52_05085"
     CDS             complement(2709..>3566)
                     /locus_tag="VKQ52_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86586.1"
                     /translation="KVDGLTLDEARNMTAGEGEFFEGEPVPVAGGTEQHQEPSAAELL
                     LRSIEEARELRESLFRINTDFAAETAVAGETPPVEAEAPIRDEESPFVLEEAAEPVME
                     TESPVQPVEEMQPEVVQPVEVQPAVAQEPVEQQVAEARPEPVGDAAVLQPEPVTQPVA
                     EPSFVFEPYHTIDYFASQGIKLTLDENPSDPLGKQLKSFTDWLKSMRRLPQKDREVIP
                     DRVLEQTVQNFAAHSIEGKEVVTETMAEVLAKQGMREKARVVYEKLSLLNPDKRAYFA
                     TKIEQLNIL"
BASE COUNT          788 a         1002 c          964 g          812 t
ORIGIN      
        1 ggtgcaaacc tctttggtgc ggccctggtt gcggataaca tagggacagg tgttgcaact
       61 gaacatcacc agcaacccgt tctcccgctt gacatcccgg aggctgatac tcttgcccga
      121 cacatctttc atcaggatat cggacttggg gagcgtggcg ccgatcggca ggcttccttc
      181 ccctgtggaa aatggataaa aagaaagaag agcaagcgcg ggcaggaaaa ggcccatgaa
      241 aaatagcttc ttcataatcc aaggtttttg tcctgtaaag ttaacgcttt tcctccgcca
      301 tcgccttcac atgatggata cttacattca attcataccc gatgaggagg accagggagt
      361 tgaaatagac caggatcatg agaatcatta cggtgccgat agagccgtag actttattgt
      421 aggtgccgaa ccggttgacc caataggaga aggccatcgt ggtcatgatg gtcagaaagg
      481 tagccagcag cgttcccggg gagaaaagcg accagcgctc ttttaccgcg gggccgtact
      541 tgtagataca ggcgatggcg aaataaaaga gcgggatgat caccacccag cgaagggtcc
      601 ggaacaacca gcggagactg tgatcgttgt tgctgatatg cagggccttc agtatgctgc
      661 gcagcaattc cgcctgggtg accaggatga tcacgctgcc gatgatcatg caaagcacca
      721 tcgtagtgag cttgatagcc atccaccgcg tttgcagggc attgcgccgg gtattgtaga
      781 taagggattt gttaaaagag cgcatgatcc cgatcatcgc gttggaagaa tagaacaggg
      841 ccagtacgaa accaaaggac aacagaccta ccctcggttt gtccaggaag tcctcgagga
      901 agcggctcac cagcacatag gtattctgat tgggggtgat atccttcgtg agcaacaaca
      961 gctgccgctc gatctggcga gacaccggaa gataggggac aagggtgcag ataaagatcg
     1021 tggcggcggg gatggccgtc aggaaactga aggcgatgct ccgggcccgg tcgatcaacc
     1081 ccactttttt tacctggctc aggaaaaagt ggatcacatc gaagagcgct ctgccctgga
     1141 aaccgggtag agtgaccttc ttactcctgg cttccaggaa gcggaaaggg gcgctgtcgt
     1201 aaagctttcg ttctagtttg atcatatccc gtcatttctt ccggccttcc ggcttcttaa
     1261 gactatcggc acccgccttg atgccttgct gcttgtcgag ggcgtcccgg taggcctttg
     1321 catattccag gtgttcctta taggtggcgg caaaattcga gtaaccggaa aagtcgggtt
     1381 tggctacaaa atacaggtag tcggtcgtcg gtgatgccag tacggcttcc agggtcggtt
     1441 ccgaaggagt acagatcggt cccggcggga gcccttcatg ctgataggta ttgtacggag
     1501 attctaccag caggtattta tcgtagatcc ggcgtaagtc gaaatcccgc agggcgtatt
     1561 tgatcgtcgg atcggcggaa agccggatac ccctggccat ccggttgaga tagacgctgg
     1621 cgatcttccc cttgtcggcc tgtgcgttcg tttcctcctc cacgatcgag gcaaggatgg
     1681 tagccgtcac cggcgtaagc cccctcgccc ttgccgcctc gacccgtgcg ggtgtccacc
     1741 aggccttgta ggtgctgtac attttcctga acagggccga tggggtcgta ttccagaaac
     1801 tggtgtacgt attggggacc acgatcgcca gaagcgtatt gctgtcgacg ccaaatgcgt
     1861 ggagcgaatc attgttatga agataactgc ccaatgctgc cgaatcgcat tccagctttt
     1921 tcccgatagc cccggccagt ccttcaaggg tccggaattt ggtgatgacg agcttcacag
     1981 gcgcctgccg gccgttgtgc agcatgcgca ggatacccac gatgctcatt cctttttgga
     2041 tctcatactt ccccgcccgc acgctccggg ggtagcccat cctgccggcc acccagttga
     2101 aaatggccgg actctgtatg acctcatcct tttttaaagt gtccagcacc tgctcatagg
     2161 tcatcccggt gtggatatac aggtaataat tgtcgccgct gaaagccgta gcagagccaa
     2221 agacccgcca gccgacaaaa catccggcga caagcagaag aagaacagag cctagaatga
     2281 atttcttcat aggtaattcg attggtgcga ttggtggcct aatttataca aaagcggcca
     2341 tctcagaaaa caattgagat ggccgcttat aaaattatct tgtcggttac ttctgtgccg
     2401 gtgcagtgcc tgcgggagcc gtaggattgc ctgcgggcgc ggtggcatta actttttccg
     2461 ggacgcgttg gctgctgccc gatgccctgg gaacgaacac ggcggagaaa agacagagaa
     2521 gacccacgac gatggcgagt gcccaggtgc ctttttccag tacgtccgtg gtctgcttaa
     2581 cccccatcaa ctggttgctg aacccaccca cgtttcctgc cagtccaccc cctttcgggt
     2641 tttgcaccaa aatgatcagg ccgagtaccc cacacgcaag aatgatcagg atcacaaaga
     2701 aaatcatatc aaagaatatt taattgttcg attttggttg caaaataagc ccttttatcg
     2761 ggattgagca aacttaattt ctcataaacg acccttgctt tctcccgcat gccctgcttg
     2821 gccagcacct ccgccatggt ctccgtcacc acctcttttc cttcgatgga atgcgcggcg
     2881 aagttctgca ccgtctgctc cagtacccgg tcggggatga cctccctgtc cttctgcggc
     2941 agccttctca tgctcttcaa ccaatcggta aaactcttta actgtttacc caaggggtcg
     3001 ctgggattct cgtccagcgt cagcttgatc ccctgcgaag caaaatagtc gatcgtatga
     3061 tagggctcga aaacaaagga gggttcggcc accggctgtg tcaccggctc gggttgcagg
     3121 acggccgcat cgccaaccgg ctcgggccgc gcctccgcga cctgctgctc aacgggctcc
     3181 tgcgccaccg cgggctgtac ttcgacaggc tgaaccacct ccggctgcat ctcctccacc
     3241 ggctgcaccg gggattcggt ctccatcacc ggctccgccg cttcctccag cacgaacggc
     3301 gattcctcat cgcggatcgg cgcctctgct tccacgggcg gggtctctcc cgcgacagcc
     3361 gtctccgctg caaaatcagt gtttatcctg aaaagggatt ctctcaactc tctcgcttcc
     3421 tcgatactac gcagcaataa ctcggcggcg gatggttcct ggtgctgctc cgttccaccg
     3481 gcgacgggca ccggctcgcc ttcgaaaaat tccccttccc cggccgtcat gtttcgcgct
     3541 tcgtcgaggg tcaggccatc tacctt
//
LOCUS       DATGFG010000273         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_44681, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000273 DATGFG010000000
VERSION     DATGFG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44681"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2095
                     /locus_tag="VKQ52_05090"
     CDS             <1..>2095
                     /locus_tag="VKQ52_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ86587.1"
                     /translation="RTEMTIGARSQFSITLTSSADKDLQDVVVVGYGTTKKSDLTSSI
                     VKVGGDKVANIPFTSIDQSLQGKAAGMQSSGFSGQPGANQQVRIRGIGSYTASTQPLY
                     VVDGIQINSGDLQNITILSTTNVLASLNPDDIESISILKDAAATSIYGARGGNGVILI
                     NTKKGRAGRTQINVTGEAGANKFGKLPTAAQPLNAKDWLALFQESYINAGRTAAQAAT
                     AAAAYGDGTVNTDWLKLVTRTGQQQQYNISAQGGEGKTTFFVSGGYFKQQASSIGDDL
                     TRWSTLLKVDHQAGQKLNLSISIQPSYTTQHGTLSNGSFFGNPVMNIFFLRPTQNPFN
                     ADGSLNINTNNAGFSSVYNPLYIVKNNIHSNGQFSGIGNAQARYSILDNLKFTSKMGI
                     QYQTYNDYEYDNPLHGDGKASNGRGFSDYAQYFLYDWTNQLDYHFNLNKDKDFTADAK
                     LGYESILSKAYFIQANSQNFPHVPLDLSTIAATPTAANTAGSDYSFASVFSNLTLNYK
                     DKYILSANFRRDGSSRFSTSNKYGDFPSVGFSWNVSKEDFMQDVAVLSGLKLRLSYGS
                     TGNAEIGNYTYIQQLGYGATLNYNSQPGGGFNALGNSQLTWEKDNQADVGIDASLFDN
                     RVSIVADYYDKESSHLLFAVPLSQTTGFTNFTENLGKMSNKGFELTVNATPVATRNFS
                     WDISVNFTYNKNAIKT"
BASE COUNT          518 a          634 c          524 g          419 t
ORIGIN      
        1 cccgtacgga aatgaccatc ggcgccagga gccaattctc gatcacgctt acctcctcgg
       61 ccgacaagga tctacaggat gtcgttgttg tgggctatgg gacgacaaag aaatccgacc
      121 ttacttcgtc catcgtcaaa gtgggcgggg ataaggtggc caacattccc tttacctcga
      181 tcgaccagtc cctgcagggg aaggccgcgg ggatgcaatc ctccgggttc agcggtcagc
      241 ccggcgccaa ccagcaggtg cgtatccggg ggatcggttc ctacaccgcc agcactcagc
      301 ccctgtatgt cgtcgacggt atccagatca acagcggcga tctgcagaac atcaccatcc
      361 tttccaccac taacgtgctg gcaagcctca accccgacga catcgagtcg atctccatcc
      421 tgaaagatgc ggcggccacg tccatctatg gggccagggg gggtaacggt gttatcctga
      481 tcaacacgaa aaaaggccgg gccggcagga cgcagatcaa cgttaccggc gaggcgggag
      541 caaacaaatt cggcaaatta cccaccgccg cacagccact caatgcaaag gactggctgg
      601 ccctgttcca ggaaagttat atcaacgccg gcagaacggc cgcacaggct gccaccgcgg
      661 cagcggccta tggcgatggg acggtgaaca ccgattggct gaaactggtc acccgtacgg
      721 ggcaacagca gcagtacaat atctctgcgc agggcggaga aggaaagacc accttcttcg
      781 tgtcgggcgg ctatttcaaa caacaggcgt cttccatcgg cgatgacctg acccgctggt
      841 ccacgctctt gaaggtagac caccaggcgg ggcaaaaact gaacctgtcc atcagcatcc
      901 agccttccta taccacacag cacggaacgt tgagcaatgg cagcttcttc ggcaacccgg
      961 tgatgaacat cttcttcctg cggccgacgc agaacccttt caacgcggat gggtcgctga
     1021 acatcaacac caacaatgcc ggtttttcca gtgtgtataa tcccctgtat atcgtaaaga
     1081 acaatatcca ctccaacggc cagttcagtg gtatcggcaa tgcccaggcg cggtacagca
     1141 tcctggataa cctcaaattc acctcgaaaa tgggcatcca gtaccagacc tataatgatt
     1201 atgagtacga caacccgttg cacggtgacg gcaaggcctc caacggccgg ggcttctccg
     1261 actatgccca gtattttctc tatgactgga ccaatcagct ggattatcat ttcaacctga
     1321 acaaggacaa ggactttacg gccgatgcca aactgggtta tgagtccatt ctctccaagg
     1381 cctatttcat ccaggccaac tcgcagaact tcccacacgt accgctggat ctttcgacga
     1441 ttgccgccac gccgacggcc gccaataccg ccgggtccga ttattccttc gcgtccgtgt
     1501 tctccaacct cacgctgaac tacaaagaca agtatatcct gtccgccaac ttcagaaggg
     1561 acggctcttc ccggttctcg acctcaaaca aatacgggga cttcccctcg gtcggcttct
     1621 cctggaacgt ctcgaaggag gacttcatgc aggatgtagc cgtgttatcc ggacttaaac
     1681 tgcggttatc ctatggttct acgggaaatg cggagatcgg taactatacc tatatccagc
     1741 aattgggtta tggcgccacg ctcaactaca acagccagcc cggcggcggc ttcaacgcac
     1801 tgggtaattc gcaactcacc tgggagaagg ataaccaggc cgatgtgggc atcgatgcct
     1861 cgctgttcga caaccgggtc agtatcgtcg ccgattatta cgacaaggaa agcagccacc
     1921 tgctctttgc cgtaccgctc tcccaaacca ccggctttac caatttcacg gagaacctgg
     1981 gcaagatgtc gaataaaggc ttcgaactca cggtcaatgc aacgcccgtc gccacccgta
     2041 atttcagctg ggatatcagt gtcaacttca cctataacaa gaatgcgata aaaac
//
LOCUS       DATGFG010000274         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1091058, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000274 DATGFG010000000
VERSION     DATGFG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091058"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="VKQ52_05095"
     CDS             complement(<1..1477)
                     /locus_tag="VKQ52_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HLZ86588.1"
                     /translation="MKSLQIIGITPLEIPDARLALHLSTSGFFPVLHLGREEERAVAA
                     LKELAEKMQEPFGVCFVSPFTRAIDLPAQVGVVIVPYGMKLTLPPGARVFYQVGTAEQ
                     AFRAKEEGADGLIIKGNEGAGWVSDESSFILLQGIVGKLADIEIWVQGGVGIHSAAAM
                     AALGVDGIVLDSQLIPFSECSAPAEIKNLCLSLNGSETKVIDNRRVLSRANFPVLPLG
                     QDVALGGALVSRYKKLHRLAFAIREAVYGHLKQAKALPPIRPGNALAKELNVHYPIAQ
                     GPMTRVSDVPAFADAVAEAGALPFIALSLLRGEKARQVMQETRRLAGGKTWGVGILGF
                     IPQELRDEQLTYILEEKPPVVLIAGGRPSQAKALEEQGIKVFLHVPSVALLDMFIRDG
                     ARRFVFEGRECGGHVGPLSSLVLWERQIDRLLQEESAESFSVLFAGGIHDAFSAAFVG
                     FMAAPLATKGAGIGVLMGTAYLYTEEAVSTGAIVRQFQEQAI"
     gene            complement(2074..>2915)
                     /locus_tag="VKQ52_05100"
     CDS             complement(2074..>2915)
                     /locus_tag="VKQ52_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86589.1"
                     /translation="ARSERMQERPNVYKDIIGDHTADVIPFEVSTIYFNGIRYSPRPP
                     IQSYAAYNRYLDSLGYAKYMSASAPDFVLFSSASIDGRCAWTDEARVKLALLDRYRLA
                     RMVDGQVVLEKRTAPRGMIKVREEVQEARMNEDIPVKKGEGIEFTQFYVQYNLKGTLR
                     SLFYHSPPINIRFTLENGETRTYKAVKPILADGIILNKFIDDADDFRLLFLSDGRMNA
                     DVRSVRIEIGNHGGFRPEIKMVNSWFVLPEKDTRERMADSVKMTEILNGRVTAPVVVM
                     RKE"
BASE COUNT          725 a          903 c          668 g          619 t
ORIGIN      
        1 cgatcgcctg ttcctggaat tgccgaacga tagcccccgt gctcaccgct tcttcggtat
       61 ataggtacgc cgtacccatc aataccccga tcccggctcc cttagtagcc agcggggcag
      121 ccatgaaccc gacaaaagca gcggaaaaag cgtcgtggat gccgccggca aaaagcacgc
      181 tgaaggactc cgcgctctct tcctgcaaca ggcggtcgat ctgcctctcc caaagcacaa
      241 ggctcgataa agggccgaca tgccctccgc attcccgccc ttcgaagaca aatcttcgcg
      301 caccgtctct gatgaacata tccagcaacg cgacagaagg aacatgcagg aataccttga
      361 tcccctgctc ttccaacgcc ttggcctgcg aaggcctccc gccggctatc agcacgaccg
      421 ggggcttctc ttcaaggata taggtcagtt gctcatcgcg taattcctgc ggaataaaac
      481 ccagtatgcc cacaccccag gtcttgccac cggccagccg ccgggtctcc tgcatcacct
      541 gccgcgcctt ttctccccgc agcagcgaca aggcaatgaa gggaagagca cccgcttccg
      601 ccacggcatc cgcaaaagca ggcacgtcac ttactcttgt catcggcccc tgcgcaatag
      661 ggtaatgaac attcagctcc ttcgccaatg cattccccgg acggatgggc ggcagggcct
      721 tggcctgctt aagatgacca tagacagctt cgcggatcgc aaaggcaagc cggtgtaatt
      781 tcttatatcg tgagaccagc gcacccccaa gggcaacatc ctgccccagc ggaagcaccg
      841 gaaaattagc cctcgacaat acccgtcggt tatcgatcac ttttgtctca ctgccattca
      901 gggaaagaca caggttcttt atttccgccg gcgcactgca ttcggaaaag gggatcagct
      961 gactgtccag cacaattccg tctacgccca aagccgccat cgcggcagcg gaatggatcc
     1021 cgacaccacc ctggacccag atctcgatat cggcaagttt cccgacgatc ccttgcaaca
     1081 ggataaagga agattcgtca ctgacccaac ccgccccctc gttacccttg atgatcaggc
     1141 catccgctcc ctcttccttt gccctgaagg cctgctccgc cgtacctacc tggtagaaaa
     1201 cccgggcccc gggcggaagg gttagcttca ttccataggg aacgatcacc acccccacct
     1261 gtgcaggcag atcgatggcc ctggtaaaag gagaaacaaa acaaacgccg aacggttcct
     1321 gcatcttttc agcaagttcc tttaatgcgg caacggccct ctcctcctcc cggcccaaat
     1381 gcagcaccgg gaaaaaaccc gaggtcgaca ggtgcaatgc aagacgcgca tcggggattt
     1441 caagcggcgt tattccaatg atttgcaaac ttttcatggc gattctatta gattaaggta
     1501 aagcacagcc tggcaacgcc tggtcggcgt aactaccatc cgatatgaca tatacaatcc
     1561 gatatgacat agcaaaacat agcatagccg ggaccgtttg tcaacggcaa acctgcgact
     1621 attgcggtta agacaagcaa tcggttgctc ctgtcatgct gcaggaaaaa atgttaaagg
     1681 gaaagggaag taatgtagtg gccaggaaat taactaataa ctaatcagaa gctggcagtt
     1741 ggattttagc aaaaaaagca agcaaaggct ggaagaaatt tttattagca ggggataggg
     1801 agagacgaca gttgcatcgt catattttga ctaaattaag aactatgact gtacacgcca
     1861 aattttcttt ggcacatgtt tgcccttttt ccttctgttt acaggcatac cattaccatc
     1921 acgcaaaata aaaaacgctg ccgcataaaa aagcgccgcg gatgatgctg ttgatcatcc
     1981 agcggcgctt atacttaggt gttattttct gcagccggct ggcggcctca aagcgggctg
     2041 actggcccga agcggcctct tggtctcgcc atatcactct ttccgcatca ccacaaccgg
     2101 cgccgtcacc cgcccattca gtatctccgt catcttcaca ctgtccgcca tccgctcacg
     2161 agtatccttc tccggtaaaa caaaccagct gttgaccatc tttatttccg gccggaaccc
     2221 gccatgatta ccgatctcga tccgcaccga ccgcacatcg gcattcatcc tgccatccga
     2281 caggaacaac aggcggaagt catcggcatc atcgataaac ttattcagga taatcccatc
     2341 ggccagtatc ggcttcacgg ccttataggt cctcgtctcc ccattctcca gcgtaaaccg
     2401 gatattgatc ggcggactat gatagaaaag actccgcagc gtccccttca aattatactg
     2461 cacatagaat tgagtgaact cgatcccctc ccctttcttc accggaatat cttcattcat
     2521 cctcgcctcc tgtacctcct cccttacctt gatcatcccg cggggcgccg tcctcttttc
     2581 caacaccacc tggccatcca ccatcctcgc cagccggtaa cgatccagca acgccagctt
     2641 aacccgggcc tcatccgtcc aggcacaacg gccatcgata ctggcagaag aaaagaggac
     2701 aaaatccggc gcgctggcgg acatgtattt tgcataacca agactgtcca ggtatcggtt
     2761 ataggcggca taggactgga taggcggccg gggcgaatag cgtatcccgt tgaaatagat
     2821 ggtcgacacc tcgaaaggaa tgacatccgc cgtatgatcc ccgataatat ccttgtagac
     2881 attgggtcgc tcctgcatcc gctccgatct cgcca
//
LOCUS       DATGFG010000275         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_450315, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000275 DATGFG010000000
VERSION     DATGFG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450315"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /gene="leuB"
                     /locus_tag="VKQ52_05105"
     CDS             complement(<1..742)
                     /gene="leuB"
                     /locus_tag="VKQ52_05105"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HLZ86590.1"
                     /translation="MASKKILIVPGDGIGQEVTAVGKKVLDAIAAKFGHSFSYDEALI
                     GHVAIEATGSPLPDESLEKMRKSDAVLFGAVGHPKYDNDPSARVRPEQGLLKMRKELG
                     LYANLRPIKLFDELLGASSIKPEILKGADILFFRELTGDIYFGEKGRKNNGDTAYDVA
                     EYSRFEVERIARKAFVAARTRRKKLCSVDKANVLETSRLWREVVQQVAKDYPDVEVEH
                     QFVDATAMLLIKDPRRFDVVVTANLFGDI"
     gene            complement(754..>2006)
                     /locus_tag="VKQ52_05110"
     CDS             complement(754..>2006)
                     /locus_tag="VKQ52_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HLZ86591.1"
                     /translation="WMQQAGAKVMNLLTKGSLNHLTHQLKKKPEQHFEEVAAVIALGR
                     KKGLECNVYLEDWSNGMRHSRDYVYQYLDFLQQQPVKRIMLPDTLGILIPSEVAEYVG
                     AIVERYPALHFDFHAHNDYDLGTANVLEGVKAGARGIHLTVNGMGERAGNAPLASAVA
                     VLHDFLPSVKTSVNEASLYRVSKLVETFSGVRIPSNKPVVGENVFTQTAGIHADGDKK
                     NKLYFSDLMPERFGRQRKYALGKTSGKANIENNLSQLGIQLSEADLKLVTQRIIELGD
                     RKEVVTQADLPYIISDILDSKAIQEKVRIENYVLTHSKNLHPSVTVKVSINGEIFEEH
                     AQGDGQYDAFMNALKKVYKRQKQELPVLVDYAVRIPPGGKTDALCETVITWAANNREF
                     KTRGLDSDQTVSAIKATQKMLNLL"
BASE COUNT          416 a          619 c          503 g          468 t
ORIGIN      
        1 ggatatcgcc aaaaagattc gccgtcacca ctacatcgaa gcggcgggga tccttgatca
       61 gcagcatcgc cgttgcatcc acgaactggt gctccacctc cacatcggga tagtccttcg
      121 ccacctgctg gacgacctcc ctccaaagcc gggacgtctc gagcacattg gccttgtcca
      181 cggaacaaag cttcttccgc cgcgtacggg ccgcaacaaa tgccttgcgg gcaatacgtt
      241 ccacctcgaa ccggctgtat tccgctacat cataggccgt atccccgttg ttcttgcgcc
      301 ccttctcccc gaaatagata tctcccgtca actcccggaa gaaaaggata tcggcccctt
      361 tcaggatctc cggcttgatg ctcgaagcac cgagcaattc atcaaataat ttgatcggcc
      421 ggagattggc atacaacccc agctccttcc gcatcttcag caggccctgc tcgggacgca
      481 ccctggcaga aggatcatta tcatatttgg gatgacctac ggcgccgaag agcaccgcat
      541 cggacttccg catcttctcc aatgattcat cgggcagcgg actgcccgta gcctcgatgg
      601 cgacatgacc gatcaatgcc tcgtcatacg aaaagctatg cccgaatttc gcggcaatcg
      661 catccaaaac tttttttcca actgcggtta cttcctgccc gattccgtcg cccggaacga
      721 tcaggatctt tttacttgcc atgctattag atattaaagt agattcagca ttttctgggt
      781 ggcctttatc gcggataccg tctggtcgct atccagcccc ctcgtcttga actcccggtt
      841 attggcagcc caggtgatca ccgtttcgca gagcgcgtct gtcttcccgc cgggtggaat
      901 acgaacggcg taatccacca ggacgggcag ctcctgcttc tgccgcttat acactttctt
      961 cagggcattc ataaatgcat cgtactgacc atcaccctgg gcatgttcct cgaagatctc
     1021 cccgttgatg gaaaccttca ccgtcaccga cggatgcagg ttcttggaat gcgtgagcac
     1081 atagttctcg atccgcacct tctcctggat ggccttgctg tccaggatat ccgaaatgat
     1141 atagggcaga tcggcctggg tcacgacttc cttgcgatcg cccaattcga tgatccgctg
     1201 ggtcaccagc ttcaggtccg cctccgacag ctggatgccc agctgcgaaa gattgttctc
     1261 gatattggcc ttgccgctgg tcttaccaag ggcatattta cgctgacggc cgaaacgttc
     1321 cggcatcagg tcgctgaaat acagtttgtt cttcttatcc ccatcggcat ggatcccggc
     1381 cgtctgggta aagacattct ctcccaccac cggtttgttg gaagggatgc gcaccccgga
     1441 aaaggtctcc accagtttgc tgacccggta gagagaggcc tcgttcaccg aggtcttcac
     1501 cgaaggcagg aagtcatgga gcacggcgac cgcgctggcc agcggagcat tcccggcccg
     1561 ttcccccatc ccattgacgg tgagatggat gccccgcgcg cccgccttca cgccttccag
     1621 cacattggcg gtgccgagat cgtagtcatt atgcgcatgg aagtcgaaat ggagagcggg
     1681 ataacgttct acgatggcgc cgacatattc cgccacctcc gaagggatga ggatgccgag
     1741 ggtatcgggc aacatgatgc gcttcaccgg ctgctgctgc aggaaatcca ggtattgata
     1801 gacatagtcc ctggaatgac gcatgccgtt gctccagtcc tcgagataga cattacattc
     1861 cagtcctttc ttccggccaa gcgcaataac ggccgccacc tcttcgaaat gctgttccgg
     1921 cttcttcttc agttggtgcg tgagatgatt gagcgagccc ttggtcagca ggttcatgac
     1981 cttcgcgccg gcctgttgca tccact
//
LOCUS       DATGFG010000276         3452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1406845, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000276 DATGFG010000000
VERSION     DATGFG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406845"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VKQ52_05115"
     CDS             <1..1145
                     /locus_tag="VKQ52_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HLZ86592.1"
                     /translation="YWYFDGKDLSFAIERMVTVIVICCPHALGLAVPLVVARSTALSA
                     KHGLLIKNRTAFENARRITTLVFDKTGTLTVGKFEVVRYQSLHQQNAPGQLPPLTDDN
                     ILQLAAALEKGSEHPIAAGILQKAKDNHLRLEDPSNVQALTGKGIAGLIKGNKVAVVS
                     PQAVPELSGGKAPGQEIKTGETLVYVLVNEQVAGFIALADQIRPESYDAIKTLHQHQI
                     KTILLTGDNKAVAEGVAKELGMDSYLAEVLPHQKLEKIKALQAQGEFVAMTGDGVNDA
                     PALAQANVGIAVGSGSDIAAETAGIVLVNSNPKDIVSLILFGKATYRKMIQNLIWATG
                     YNVIALPLAGGILYKQGILLSPAAGAVLMSLSTVVVAINASMLKVK"
     gene            1170..2126
                     /locus_tag="VKQ52_05120"
     CDS             1170..2126
                     /locus_tag="VKQ52_05120"
                     /inference="COORDINATES: protein motif:HMM:NF025879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ86593.1"
                     /translation="MPVIPADPERKRRIDRVLDYIFSHLDGQISLQTLAGIAHYSPFH
                     LQRIFKQMVGDSPKQYIIKLRLETAFHLLVIHPQKSIQEIAIDSGFSHPTVFSRATKS
                     YFGYSPEQIRMLPHREQMRLLHTVAPQSTPPAALAPTTGRASTAETQSIAPSIAPPSI
                     DNPTRRKPTIHTLKKAAITGIYLLVPFDNPIEIQRAFATLSRLANIHAWPTPEPGMYG
                     ILGPHQRNTYKAFLPIDPAAFAADKFPLTRIKGGTFATFNVRGHLRQTNKAAHHFYRQ
                     WLPDSGYKIAGVTGFETFSGNPAFTPYHQLEREIHIPIEPAL"
     gene            2219..2341
                     /locus_tag="VKQ52_05125"
     CDS             2219..2341
                     /locus_tag="VKQ52_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86594.1"
                     /translation="MTLKTKPPTPRWVKISGLITLVLLLGIIIKHLTGHNFHHH"
     gene            2341..2883
                     /locus_tag="VKQ52_05130"
     CDS             2341..2883
                     /locus_tag="VKQ52_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86595.1"
                     /translation="MTLSSPLRKLALLVHVTTSIAWPGAIAAFLALAITGLTSTDTQI
                     VRSVYVAMPPITWYVIVPLAFAALLTGLLLSLGTKWSLLRHYWVLIKLLINAVSIPIL
                     LLHARIIQEVASAAEKTTLSGTDLHDQRVQLVTVSIAALLALIIATILSLYKPRGITP
                     YGWSPKKKEDSLVRKEYFSE"
     gene            3105..>3452
                     /locus_tag="VKQ52_05135"
     CDS             3105..>3452
                     /locus_tag="VKQ52_05135"
                     /inference="COORDINATES: protein motif:HMM:NF045002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86596.1"
                     /translation="MRKLASIQMIRSLEAIEGADAIEKATVMGWQLVVKKGEFRVGDL
                     CIYCEIDSMMPDRPEFEFLRPRGMRIKTIRLRGQVSQGICLPISILPAGTVLEENTDC
                     TDILGITKYAPPMP"
BASE COUNT          877 a         1124 c          737 g          714 t
ORIGIN      
        1 cttactggta ttttgatgga aaagacctgt ccttcgctat cgaaagaatg gtcaccgtca
       61 tcgtcatctg ctgtccccac gccctgggcc ttgccgttcc cctcgtagta gcaagatcca
      121 ccgccctatc tgcaaaacac ggtttactca tcaagaaccg gacagccttc gagaatgccc
      181 gacgcatcac cacccttgta ttcgataaga ccggcaccct caccgtcggg aagttcgagg
      241 tcgtccgata ccaatccctc caccaacaga acgcccccgg ccaactcccg ccactgacgg
      301 atgacaacat cctgcagctc gccgcggccc tcgaaaaagg ttccgagcac cctatcgccg
      361 ccggtatcct tcaaaaggca aaagataacc acctccggtt agaagatcca agcaatgtcc
      421 aggcccttac cggcaaggga atagcaggcc tcataaaagg caataaagtg gcggtcgtca
      481 gtccgcaggc cgttcccgaa ctgtccgggg gcaaggcccc gggccaggag atcaaaaccg
      541 gcgaaaccct ggtatatgta ctggtgaacg agcaggtcgc aggtttcata gccctggccg
      601 accaaatacg gccagaatcc tatgacgcca tcaaaaccct gcaccaacac cagatcaaaa
      661 ccatcttact taccggggac aacaaagcag tggccgaagg tgtcgccaaa gagcttggca
      721 tggacagcta tcttgccgag gtattaccgc atcaaaaact cgaaaaaata aaagcattgc
      781 aagcccaggg ggagttcgtt gcgatgaccg gcgacggcgt caatgatgcg cccgccctcg
      841 ctcaggccaa tgtcggcatc gccgtaggca gcggtagcga catagccgcc gagactgcgg
      901 ggatcgtcct ggtgaacagc aatcccaaag atattgtcag cctcatcctc ttcggcaagg
      961 ccacttatcg taaaatgata cagaacctta tctgggccac cggttataac gtcatcgcgc
     1021 tgcccctggc cggcggcata ttatacaagc aggggatctt gttaagcccc gccgccggcg
     1081 ccgtcctgat gagcctgagc accgtcgtcg tcgctattaa cgcgagtatg ctgaaagtaa
     1141 aataaatttg acaacaaaac catgcaccta tgcctgtgat cccagccgac ccggagcgca
     1201 aaaggagaat cgaccgggtt ctcgactata tcttcagcca cctcgacggc cagatctccc
     1261 tgcaaactct tgccggcatc gcccactatt ccccctttca cctgcaacgc atattcaaac
     1321 agatggtcgg ggactccccc aaacaataca tcattaaact tcgcctcgaa accgccttcc
     1381 acctcctggt gatccatcct caaaaatcca tccaggaaat tgccatcgac agcggcttct
     1441 cccaccccac cgtattctca agggccacga aaagctactt cggctactcc cccgagcaaa
     1501 tacgcatgct gccccataga gaacagatgc gactgctgca taccgttgcc ccccaatcca
     1561 ctccacctgc agccctggca cccaccaccg gacgggcctc aaccgctgaa acgcaatcta
     1621 tcgctccatc tatcgctcct ccctccattg acaacccgac acgccggaag cctaccatcc
     1681 ataccctcaa aaaagcagcg atcaccggca tctacctcct tgtccccttc gataatccca
     1741 tagagatcca gcgtgcattc gccacactct ccaggctcgc caacatacat gcctggccaa
     1801 cccccgagcc gggcatgtac ggtatattag gcccgcacca acggaacacc tacaaagcct
     1861 tcctccccat tgacccggca gccttcgcag cagacaagtt cccccttacc aggatcaaag
     1921 gaggaacctt cgccaccttc aacgtccggg gccacctgag acagaccaat aaagcagcgc
     1981 atcattttta ccgccaatgg cttcccgaca gcggttacaa gatcgccggc gtcaccggct
     2041 ttgaaacctt ttccggcaac ccggctttca ctccttacca ccaattggaa cgggaaatcc
     2101 atatacccat cgagcccgct ctataagaat cgcaagattt gcaacgtctt tccccggttt
     2161 tgtcatgcta taaccgcggc tatctcccaa ttttaccgga gaaaacagca ataccgccat
     2221 gacactaaaa accaagccac ccacaccccg ctgggtaaag atatccggcc tcattacgct
     2281 tgttctcctc ctcggcatta tcatcaaaca cttaaccggc cacaacttcc accaccactg
     2341 atgacactat cctctcccct ccggaagctg gccctcctcg tccacgtcac cacctccatc
     2401 gcctggcccg gcgcgattgc cgccttcctg gccctcgcta tcaccggcct caccagcacg
     2461 gacacgcaaa tcgtccggtc cgtctacgtg gccatgccac ccatcacctg gtatgtgatc
     2521 gtacccctcg cctttgccgc cctgctcacc ggcctccttc tatcgcttgg aacaaaatgg
     2581 agcctgctac ggcattattg ggtcctgatc aaactcctga taaatgcagt ttccatcccc
     2641 attctgctgc tccacgcccg gatcatccag gaggtagcca gtgcagccga aaagaccacc
     2701 ctgtccggca ctgatctcca cgaccaacgc gtccaactcg taaccgtctc catcgccgcc
     2761 ttgctggcat tgatcatcgc cacgattttg tccctctaca agccccgggg cattacccca
     2821 tacggttggt caccgaagaa aaaggaagat tctctggtac ggaaagaata tttctccgaa
     2881 taattcactt ttcttaattt cgatttctac ttcgttgttc catcagccaa taactagttc
     2941 ctccatacac cttcttttca acgggagcta aggaacaaga catcttactt cactacgagc
     3001 taacggggag cttgttattc ttcaaaatga cacaatcgac tgccatgtgt catcccgcac
     3061 acgcgggaat tttactcaca cataaagagc tcccgtaagt aaatatgaga aagttagcga
     3121 gtatccaaat gatcaggtca ttggaagcaa ttgaaggtgc agatgccatt gagaaagcaa
     3181 cggttatggg ctggcagttg gtcgtaaaga aaggcgaatt ccgggttgga gatctgtgca
     3241 tttactgcga gatcgacagc atgatgcccg atcgacccga atttgaattt ctcaggccca
     3301 gaggaatgag aattaaaaca atacgattgc gtgggcaggt ttcacaggga atttgtttgc
     3361 caatttcgat tctgccagcc ggaaccgttt tggaggaaaa taccgactgc accgacatcc
     3421 tcggaattac aaaatatgcc cccccgatgc cg
//
LOCUS       DATGFG010000277         6357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_499790, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000277 DATGFG010000000
VERSION     DATGFG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499790"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            569..3007
                     /locus_tag="VKQ52_05140"
     CDS             569..3007
                     /locus_tag="VKQ52_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86597.1"
                     /translation="MIRNYLLTAWRNLQKNKLNATINVIGLSVAFTCCILLFLMVRYE
                     FSYDDFQRNGDKLFIAYNLSHEAQGDTRGTSMSFRAAPAMKKEIPAVVRSTNIMSAGG
                     GIRYKGKEVYHRVTLVDPDFFRMFSFPIVAGNSSAPLGSGSDVVIAQATAKALFGEED
                     AVGKPVEIKVGGQWKAVRVSAVIADAPQNSTLQYSVLARCEIYPNYPQEKDNWNITHH
                     PVYVQLSSAGGQRAAERDLRQLAKRHGLVDEAQVRSQGYRKDENGEYASIRLESLTDL
                     HFDEELGGRNSINKTYLYTLVLIAIVVMVIACFNFINLNVARSFTRAREVGIRKTIGA
                     ERRQVYLQMWVESLLLFLSAVVIAVFAASMLLQPFNDLFTEKLRLNTLVQTKVLLTAL
                     AGTILVSLLAGGYPAWLVSRFNTVEVLKGKVSVKRSGLLRNGLITFQFILASALISGT
                     LVIYRQFTHLRTASLGFEQESVISIPVRNGENARRYIDEMRMKLASQPQVVAVTGSSV
                     NIGIGEDKSQSMHGVGFNYKGQGIITATLGIDYDYLSVLGMKTIAGRDFSRAYATDTS
                     SAVTNVIVSESMARQFGIRNMQDAVGLSFYPDSSKPKWTIVGVIPDFHLYSMNDRLQP
                     LTLLLNRHQGVDYIFVKVRTTNPVAAMQLVQSTFHGLEPDNAVNPSYLTENTARWYDK
                     ERRLSSIFFTSAFIAILLCCLGLFAMVSLVMEQRRKEIGVRKVLGASIPSITSLLSRD
                     FIRLVAIAFLVSTPVAWYFLDQWLRNFEYRVDASWWVFALAGVVTLLIALVTVGIQTV
                     RAAVVNPVRSLRSE"
     gene            complement(3122..5476)
                     /locus_tag="VKQ52_05145"
     CDS             complement(3122..5476)
                     /locus_tag="VKQ52_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86598.1"
                     /translation="MRVFAGALALLLITAAAGAQSRILGTITDNRSQPVANASIHLLN
                     TEITAISDSAGDFAIPHLPAGRYVLQLSAIGYTTLAAPLDITAGDNKREFRLSNSLTQ
                     LDAVTVTADKKEELLQTVPLSVTAIPARQVEDFRLWDIKGITAIVPNLYSGNSGDGRN
                     VTSIRGITTTSYDPAIATYIDGVNQFSLDTYIPELNDIERIEILRGPQGTLFGRNAMG
                     GVINIITRQPTNATHGFVQIDEGNYGRQRYAAGIRTPLIKDVLFLGVSGVYSKRDGYY
                     TNEYNNSSFDRQSALTGNYYLKYLPGAGWSVTLNVKHQNNRNWGAFPLSTSAADAFSH
                     PFRLDQNAIGKMIDNTLNASLSIKHAGATLDFSSQTSWQNNHRYYQPSVDGDFSPADI
                     VTIVNDYGSAWNNVQVFTQEFRLGSPAHRNTPLSWTAGAYFFHQYNPNKQATHYGTQA
                     GLYGVPDSNFSSIDITKGKNTGVAGYAQVNYAVTEKWQLIAGLRYDYEHRYLDVTEQF
                     QPDGRPAITTIPDTSAAVHFSALSPKAGIQYQPTAHLNLFATYSRGYRPGGLTQLSSD
                     PSQPPLYPYQPEYSNNIEIGIKNTWWDHRLELNATAFLSHVNDAQVPTLILPSAITVT
                     RNTGKLISKGLELEAAATPVKGLHLLYTFGYTDAHYQSLPIAENGQAVDLAGKKQIFT
                     PDITSLFAPQYSVGLSERHQLRLVLRAEWFYLGREYFDLANTITQAPHSILNARAGLT
                     IPHLELFFWTRNLTNTKYIEYAYDFGAVHLGDPHTWGITLRTNW"
     gene            complement(5480..>6357)
                     /locus_tag="VKQ52_05150"
     CDS             complement(5480..>6357)
                     /locus_tag="VKQ52_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLZ86599.1"
                     /translation="LALGKQAQAFFNTPRAQHPIGLQLFTLFMIIDDDVPGNLKKVAA
                     IGYREIESAFSKKGGYYGMKPKQFAALVKDTGMTWRSHHVLGAPFHLPPNAKPFLDAN
                     GKPITFPATMPNLKENRQQLVDEAAEGGIGYLVCANTPMHTSDEIKGSIEVLNKTGEA
                     CKKAGITLCYHNHIEEFTAVDGKRPYDLFLQQLTPDIKMELDLCWATKAGVDPVDLFK
                     THPGRFPLWHVKDLDKTRTGPAPVGTGVVDFKRIFANASTAGMKHFFVEHDMPADPFA
                     SITTSYNNLTKILSA"
BASE COUNT         1211 a         1454 c         2229 g         1463 t
ORIGIN      
        1 ttcatcccgg atcgacggat cgagcggaag gtgtccggag cggatgccgc ggaagtgggc
       61 taaaggaagc cgccggaacg taggctaaac gagccagccg ggatgtggat tttttaaaaa
      121 aaaccgggga aagtagacac aatccccggt agcttttatg ataaaacgaa cgcgcatgcg
      181 gctgggacgg cagaccgtcg cgagccggat gatccgttaa aatacatgac gggcgtggaa
      241 attaaaaaaa tgaaaatgag cagggggaaa agatgtgggg aaagataagg agatggagca
      301 ggggtaggag tgaagtgggc ggcgggcgcc ggggatggag gggctgagga tggaggtgag
      361 tgggtgtgag ggtgcgggcg aaggcgggag gcgatagggc gaggggagtg ggtgtgaggc
      421 cgcgggtggc gccggggcga gggccgaaat ggaacagcga gtgtattaaa aacgaacagt
      481 ttcgtttttg gggtcgatta taagatgctg ataggcaatg ggctaaaaag atggtcgccg
      541 atttgtgccc tgttgtaaaa atcgcttcat gatccgaaac tatctcctca ccgcctggcg
      601 taacctccaa aagaacaagt tgaatgcgac gatcaatgtc atcgggcttt cggtggcttt
      661 tacctgttgt atcctgcttt ttttgatggt acggtatgag ttttcctatg acgatttcca
      721 gcgaaacggc gacaagctgt ttattgccta caacctttcg catgaggcgc agggtgatac
      781 gcgggggacc agcatgagct ttcgcgcggc gccggcgatg aagaaggaga tcccggccgt
      841 cgtccggagc acgaatatta tgtcggcggg tggtggtatc cgttataagg gtaaggaggt
      901 gtatcaccgg gtaaccctgg tggacccgga ttttttcagg atgttctctt tcccgatcgt
      961 cgcggggaac agcagtgcac cgctgggcag cgggagcgat gtggtgatcg cccaggccac
     1021 ggcaaaggcc ctgttcggtg aggaggatgc tgtcggcaag ccggtggaga tcaaggtcgg
     1081 ggggcaatgg aaggcggtgc gggtctcggc cgttatcgcg gatgcgccgc agaattcgac
     1141 tttacagtac agtgtcctgg cgcgttgcga gatctatccc aactatccgc aggagaagga
     1201 caactggaat atcacgcacc atcccgtcta tgtgcagctt tcttccgccg gcgggcaacg
     1261 ggctgcggag cgggatctcc ggcagctggc gaagcggcat gggctggtgg acgaggccca
     1321 ggtgaggagc cagggctaca ggaaagatga gaacggggaa tatgcttcga tccggctgga
     1381 gtcgctgacc gatctgcatt tcgatgagga actgggcggc cgtaattcga tcaataagac
     1441 gtatctctat acgcttgtgc tgatcgccat cgtggtgatg gtgatcgcct gtttcaattt
     1501 catcaacctg aacgtggccc ggagctttac acgggcccgg gaggtgggga ttcgcaagac
     1561 gatcggcgcc gaacgccggc aggtgtacct gcagatgtgg gtggagagcc tgttgctctt
     1621 cctgtccgcc gtggtgatcg cggtctttgc ggcttcgatg ctgttgcagc ccttcaatga
     1681 cctgttcacg gaaaaattgc ggctgaacac cctcgtgcag acgaaggtgt tgctgacggc
     1741 gctggccggg acgatcctgg tctcccttct cgcgggcggc tatcctgcct ggcttgtgtc
     1801 gcggttcaat acggtagagg tgctgaaggg gaaggtctcg gtgaaacgaa gcgggttgct
     1861 gcggaatggc ctgatcacgt tccagttcat cctcgccagc gcgctgatca gcggcacgct
     1921 cgtcatttac cggcaattca cgcatctgcg gacggcttcg ctgggtttcg aacaggagtc
     1981 cgtgatcagc atcccggtgc gtaacggcga gaacgcgaga cggtatatcg atgagatgcg
     2041 gatgaagctg gcgtcacagc cgcaggtggt tgcggtgacg ggaagctcgg taaatatcgg
     2101 tatcggcgag gacaagagcc agagtatgca tggggttggc tttaactaca agggccaggg
     2161 tatcataacg gccacgctcg gcatcgacta tgactatctt tcggtattgg ggatgaaaac
     2221 catcgccggc cgggatttca gccgggccta tgcgaccgat acatcctccg ccgtcacgaa
     2281 tgtcatcgtc tccgagagta tggcccggca attcgggatc aggaacatgc aggatgcggt
     2341 gggtctttcc ttctatcctg acagttccaa gcccaagtgg acgatcgtcg gggtcatccc
     2401 ggacttccat ttgtattcga tgaatgacag attacaacct ttgacgctgt tgctgaaccg
     2461 gcatcagggt gtggattata tttttgtcaa ggtgaggacc accaacccgg tggcggcgat
     2521 gcagctggtg caatcgacct tccatgggct ggaaccggac aatgcggtga acccgtccta
     2581 tctgacagag aatacggccc gctggtatga caaggagcgg cggctatctt ccatcttctt
     2641 tacttcggcc tttatcgcga tcctgctttg ctgtctgggg ctttttgcaa tggtttcgct
     2701 ggtgatggag cagcggcgta aagagattgg ggtacggaag gtgctgggag cgtcgatccc
     2761 atccattacg agcctgctat cgagggattt tatccggctg gtggcgatcg cgttcctggt
     2821 ctcgacgccg gtggcctggt attttctcga tcagtggttg cggaacttcg agtaccgggt
     2881 ggacgcttcg tggtgggtgt ttgcgctggc gggggtggtg acgctgctga tcgcgctggt
     2941 gacggtgggg atacagacgg tgcgggcggc ggtggtgaat ccggtgaggt cgctgaggag
     3001 tgagtgaacc gctccgcgga ggacggtacc aggataactt tgcaaggcgg ccttgcaaaa
     3061 ttgagtcacc gcccgtaggg taagccaggg caaccgattt ggaatcctca aggaaggcgg
     3121 attaccaatt ggtgcgtagg gtgatgcccc aggtgtgggg gtcgccgagg tggacggcgc
     3181 cgaagtcgta ggcgtattcg atgtatttgg tgttggtgag gttgcgggtc cagaagaaaa
     3241 gttcgaggtg gggaatggtc agaccggccc gggcgttgag gatgctgtgg ggcgcctggg
     3301 tgatggtgtt ggcgagatcg aagtattccc ttccaagata gaaccattcc gcgcggagga
     3361 ccaatctcaa ttgatggcgc tcgcttagtc cgacgctgta ttgcggggcg aagagggagg
     3421 tgatgtccgg ggtgaagatc tgttttttgc cggcgaggtc gacggcctgg ccgttctcgg
     3481 caataggtaa ggattgatag tgggcgtcgg tgtagccgaa agtatagagc aggtggaggc
     3541 cctttacggg agtagcggcg gcttcgagtt cgaggccctt gctgatgagt ttccctgtat
     3601 tgcgggtaac ggtgatggcg ctggggagga tcagggtcgg cacctgggcg tcgttgacat
     3661 ggctgaggaa ggccgttgca ttgagttcga gccgatggtc ccaccaggtg ttctttatcc
     3721 ctatctcgat gttgttgctg tattcgggct ggtagggata gaggggtggc tgggaaggat
     3781 cggatgatag ctgggtcagg ccgccgggtc gatagccgcg gctataggtg gcgaagaggt
     3841 tcaaatgggc cgtgggctgg tattggatgc ccgcctttgg cgagagtgcg ctgaaatgca
     3901 ccgccgccga ggtatcgggg atagtcgtga tggcgggccg gccgtccggc tggaattgct
     3961 ccgttacgtc gagatagcgg tgttcgtagt catagcggag gccggcgatg agttgccatt
     4021 tttccgtgac cgcgtagttc acctgtgcat agccggctac gccggtgttc tttccttttg
     4081 tgatatcgat gctggaaaag ttggagtcgg ggacgccata gaggccggcc tgcgtgccat
     4141 agtgggtcgc ctgtttgttg ggattgtatt gatggaagaa atacgccccg gcggtccagc
     4201 taagcggggt gttgcggtga gcgggagagc cgagacggaa ctcctgcgtg aaaacctgta
     4261 cgttgttcca tgcggagcca tagtcgttga cgatggtgac gatatccgcc ggggagaagt
     4321 cgccgtcgac ggaaggctgg taataacgat ggttgttctg ccagctggtc tgggagctga
     4381 agtcgagggt ggcgcccgca tgctttatcg aaagggaggc gttcagggta ttgtcgatca
     4441 ttttcccgat ggcgttctgg tccagccgga agggatggct aaaggcgtcg gccgcgcttg
     4501 tagagagggg gaaggcgccc cagttgcggt tgttctgatg ttttacgttt aacgtcaccg
     4561 accagcccgc gcccggcagg tatttgagat aatagttccc ggtgagggcg gactgccggt
     4621 caaaagagct gttgttatac tcatttgtat agtagccgtc gcgtttgctg tagacacccg
     4681 agacgccgag gaagaggaca tcctttatca gcggcgtgcg gataccggcg gcgtagcgct
     4741 gccggccgta attgccctcg tcgatctgta cgaagccatg ggttgcgttg gtgggttgcc
     4801 gggtgatgat attgatcact ccgcccatgg cattgcggcc gaagagggtg ccttggggcc
     4861 cccggaggat ctcgatgcgt tcgatatcgt tcagttcggg gatataggtg tcgaggctga
     4921 attggttcac cccatcgatg taggtagcta tcgcggggtc ataggaggtg gtcgtgatgc
     4981 cgcggatgga agtcacgttc cggccgtcgc cggagttgcc cgaatagaga ttggggacga
     5041 tggcggtgat accttttata tcccaaaggc ggaagtcctc tacctgtctt gccgggatgg
     5101 ccgtcacgct gaggggtacg gtctggagca gctcttcctt tttgtcggcg gtgacggtga
     5161 cggcatcgag ctgggtcagg gaattggaga gccggaattc ccgcttattg tcgccggcgg
     5221 ttatgtcgag gggcgcggcc agggtggtat agccgatggc ggacagttgc aggacatatc
     5281 ggcccgcggg gaggtgcggg atcgcgaagt cgccggcgct gtcgctgatg gccgtgatct
     5341 cggtgttgag caggtggatg ctggcgtttg cgacaggttg ggatcggttg tcagtgatgg
     5401 ttccgaggat gcgggattgg gcgccggccg cggctgtgat cagcagcagg gccagggcgc
     5461 cggcgaatac tctcatgttt taggcactca ggattttggt aaggttgtta tagcttgtcg
     5521 tgatgctggc aaacgggtct gccggcatat cgtgttccac aaagaaatgt ttcattccgg
     5581 ccgtggaggc attggcaaag atgcgtttga aatcgacgac gccggtgccg acgggggccg
     5641 gtccggtcct tgtcttgtcg agatccttta cgtgccagag cgggaaacgg ccggggtgtg
     5701 tcttgaagag atcgacggga tctacgccgg cctttgtggc ccagcaaaga tcaagctcca
     5761 ttttgatatc gggggttagc tgttgcagga agagatcgta gggtcttttg ccgtccacgg
     5821 cggtgaactc ttcgatgtgg ttgtggtagc agagggtgat gcctgctttt ttacaggctt
     5881 cgcccgtctt gttgagcacc tcgatggagc cttttatttc atcgcttgtg tgcatcgggg
     5941 tgttggcgca gacgagatat ccgatcccgc cttccgcggc ttcgtctacc agctgctgcc
     6001 tgttctcttt gaggtttggc atcgtcgccg ggaaggtgat gggttttccg ttggcgtcga
     6061 ggaagggttt tgcgttgggg ggcaggtgga agggggcgcc gaggacatgg tgcgatctcc
     6121 aggtcatccc ggtatctttt acgagggcgg cgaactgttt tggcttcatc ccataatagc
     6181 cgcctttttt gctgaaggcg gactcgatct ccctgtagcc gatggcggcg acttttttca
     6241 gattgccggg gacgtcgtcg tcgatgatca tgaagagggt gaagagctgc agaccgatgg
     6301 gatgttgggc cctgggcgtg ttaaaaaagg cctgtgcctg tttgccgagg gcgaggc
//
LOCUS       DATGFG010000278         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1520838, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000278 DATGFG010000000
VERSION     DATGFG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520838"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VKQ52_05155"
     CDS             complement(<1..866)
                     /locus_tag="VKQ52_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86600.1"
                     /translation="MLPSKYLFFLLVASTCLSCRNSGPERLTPTAFCRHPKKIVVYKE
                     NVYDLNGYADEGGGDPFRLFDENDGVDPGNEPDAADGPHTNPQPTLQPAIYFPPGKGN
                     RIVIDLQTTYRLAAVYLYDRSRSPDSVWIYTGDMLHWKQKAAFTAISWFTSSCWRKFP
                     VGDSSRYVLIRFSSPKTNITEMVLYGSPDGAVPPPPSHPSGAPAFTGKPMKEFLGVND
                     YSGTVGAKWMKPFYYSRLYSFAVDFDRDTVHAYPQVRYNMTHFGFWDDSLHDYHYRTE
                     DNVRQNHHMVWYT"
     gene            complement(873..1226)
                     /locus_tag="VKQ52_05160"
     CDS             complement(873..1226)
                     /locus_tag="VKQ52_05160"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HLZ86601.1"
                     /translation="MPKKKERLIAVSPSGKKDGSMADLIHDLVRQIPRGRVTSYGAIA
                     AVLNIPNPRMVGRAMHTGDGTGVPAQRVVNSTGHLTGGFRDLRKKLLVKEGVAVKGDK
                     VVDFKILFWDPAKEL"
     gene            1488..>2132
                     /locus_tag="VKQ52_05165"
     CDS             1488..>2132
                     /locus_tag="VKQ52_05165"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HLZ86602.1"
                     /translation="MRNATFEKLRAEGLIADSSLRRVEDFGSRRLFSLFWELRLLLYL
                     GVLLLTGGLGVLVYKNIDTIGHQAVLAFIALLTAGCFLYCLRKKAPFSWAQVKAPTPF
                     FDYILLLGCLSLLIFLGYWQFEYGIFGDNYGLATFIPMVILFGAAYYFDHLGVLSLAI
                     TNLAAWAGIAITPLTILKSGNFGDSRLIYTGLLLGAVLLGAGRLTEQRGWKTHFG"
BASE COUNT          477 a          570 c          585 g          500 t
ORIGIN      
        1 gtataccaca ccatatgatg gttctgcctt acattgtcct ccgtcctata atgatagtca
       61 tggaggctgt cgtcccaaaa accaaaatgg gtcatattgt atctcacctg cggatacgca
      121 tgcacggtgt cccggtcgaa gtcgacggcg aaggaataca gacggctgta gtaaaaaggt
      181 ttcatccatt ttgcccccac cgttccgctg tagtcgttca cgccgagaaa ttccttcatt
      241 ggctttcccg taaaggcagg agcgccggag ggatgcgaag gcggaggagg gacggctccg
      301 tcgggagatc catacaggac catttcggtg atattggttt taggactgct gaaccggatc
      361 agcacataac gcgaactgtc cccgacggga aacttccgcc agcaggagga ggtgaaccag
      421 gagatggcgg taaatgccgc tttctgcttc cagtgcagca tatcgccggt atagatccag
      481 acactgtccg gggaacggct ccggtcgtag agataaacag ctgccagccg gtaggttgtt
      541 tgcagatcga taacgatcct gttgcccttg cccgggggga aatagatcgc cggctgcaac
      601 gtgggttgcg ggttggtatg cggcccatcg gcggcatcgg gttcgtttcc cggatcgacc
      661 ccatcgttct cgtcaaagag ccggaacgga tcgcccccgc cttcatcagc atatccattg
      721 agatcatata cattctcctt ataaacgacg atctttttcg ggtggcggca aaaagcagtc
      781 ggcgtcaatc gctccgggcc actattccgg caggataaac aggtggaggc gacaagcagg
      841 aagaaaagat attttgatgg gagcataagg aattacaact ctttggcggg atcccagaac
      901 aagatcttga agtcaacgac tttatcgcct ttgacggcga ctccctcttt taccagcagc
      961 tttttccgca ggtcgcgaaa cccgccggtg agatgcccgg tgctgttgac gacgcgctgg
     1021 gcggggacgc cggtaccgtc accggtgtgc atggcgcggc ccaccatcct gggattggga
     1081 atattcagga cggcggcgat cgcaccgtaa gaggtgaccc ggccacgggg gatctgtcgc
     1141 acgagatcat ggatcagatc ggccatcgac ccgtccttct tcccggatgg gcttacggcg
     1201 ataagccgtt ctttcttttt tggcatcccg aaaagtaaat cattttcatg gtaatcgtat
     1261 agcaaaaaaa aacccctgta gaaacagggg tcgtaaccaa aactaactgc ttatgagaaa
     1321 attgactgct taaaaactaa ataataacgc tgattattac aacccaaagg tacgacgcct
     1381 gcacacagtt tgctgttgca gcactgttaa tatgacaata agaaaacgtt aaaatgaccc
     1441 ggccatagat cctgctgaat atgaagaatt tgtagtagtt tgggaccatg cgcaacgcta
     1501 cattcgaaaa actccgcgcc gaagggctga tcgctgacag cagcctccgg cgggtggagg
     1561 acttcgggag ccgccggctc ttctcccttt tctgggaact gcggctcctg ctctacctgg
     1621 gggtgctgct gcttacaggc gggctgggcg tcctggtcta taagaacatc gacaccatcg
     1681 gtcaccaggc cgtgcttgcg ttcatcgcgc tgttgacggc cggttgcttt ttgtattgcc
     1741 tgcgaaagaa agctcctttt agctgggcgc aggtgaaggc ccccacccca ttcttcgatt
     1801 atatcctttt gctgggctgt cttagcctgc tgatctttct cggctattgg caatttgagt
     1861 acggcatctt tggcgacaac tacggactgg ccacgtttat ccccatggtc atccttttcg
     1921 gtgcggccta ctatttcgat caccttgggg tgttaagcct ggccatcacc aaccttgccg
     1981 cctgggcagg catcgctatc actccgctta cgatcctgaa gagcgggaat tttggcgatt
     2041 cccggctgat ctatacaggc ctcctgctcg gcgctgtcct attgggcgcc ggccggctga
     2101 cggagcaacg gggctggaaa acccacttcg gg
//
LOCUS       DATGFG010000279         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_149581, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000279 DATGFG010000000
VERSION     DATGFG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149581"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3623
                     /locus_tag="VKQ52_05170"
     CDS             <1..3623
                     /locus_tag="VKQ52_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HLZ86603.1"
                     /translation="QLVEYKDQQGKVVLKKVQSWDSPSAGPSGWLNTYYVYDDLDNLR
                     FVIPPKAVEWLEANSWNFSASSGSQVAAELCFRYEYDYRRRMAIKKIPGAGEVWMVYD
                     SRDRLVMTQDSVLRSQQKWLFTRYDVLNRPDSTGLITDPANYNNLAYHQNLAGSSTAY
                     PNLSSYTTEILTGTFYDGYSGISGVSGLPSSMATGVAGNSSYFVTNYNAAPIYAVPIT
                     AYPITRGEVTGTMIKVVGTANQYLYSESFYDDRARAIQAQSINYTGGADTVTTQYDFS
                     GKPLRTLLGQAKPTNTAQYHRVLTKMNYDAGFRVTSIWKNIDGAAADQLIDSMQYNEL
                     AQPRAKYLGNNLDSLIYDYNIRGWMTGINKGYVGGTTSHYFGMELGYDKASSVAGTSY
                     TTPLYNGNIAGTVWKSAGDGIDRKYDFSYDNVNRLTGAAYLDNHSGSGWDRSAMDYTV
                     DSLSYDANGNILSMNQHGFVMGNPTGDIDQLLYSYQTNSNKLSQVTDAMNNPNSNLGD
                     FHYSGTKQAYDYTYDGNGNLSLDNNKNIDHIVYNYLNLPQQVHMNTKGNILYTYDAAG
                     NKLQKQVIDSTSNVATTTLYLDGFQYQRRTPLSTPSIGVDTLQFMGHEEGRARWAFHK
                     YTNGDSAYAWEYDFMEKDHLGNTRVLLTQQKDTAQYLATMEPQYYSTENALFYNMYTQ
                     APIPTDFPAEPNGPVNPNQYAVGLDGYTWTVGPAIVLKVMSGDSLAIACNSFYRGGGT
                     VPSPKNSFPEALNSLAEGLVAMTGGTHGSVSDLKAANSPVAEALTSFLTTQDSNTTNR
                     PKAYLNWMLLDNQFNYVSDNGQSGAIIASNPNTLNTIATTVGIKHSGFLYIWVSSETP
                     FWQMYFDNLSVQHIAGPMVEENHYYPFGLTMAGISDKAIKTQYAENKYRYNGKELQNQ
                     EFNDGSGFEEYDYGARMMDPQLGIWHGIDPLADKSRRWSPYVYAYNNPIRFIDPDGME
                     GETYGYGHTDTESEYWNSENYEGGLSVQTSKGQISAGGGTSGGKNGGGGKKKNEKEKS
                     TVQKYDGSGLIPTPQKDKIKLPESIEHLKEFMEKTKLGEKIETAGTMNEWSGKLSKIG
                     GYDKLSEHLEIIGKGFSLVNAVNHYLEGKYFEGTLDLASMTKLSPYVFVYETVNTVVT
                     SKFTLNQAAYDADQTANQFLNAAHAARVSGDDAAAAEYWNEAARYEAIRNRILSQKNK
                     DK"
BASE COUNT         1050 a          932 c          946 g          824 t
ORIGIN      
        1 accagctggt cgaatacaag gaccagcagg gaaaggtggt gctgaagaag gtgcagtcct
       61 gggacagtcc ttctgccggc ccttccggct ggttgaatac gtactatgtc tatgatgatc
      121 tcgacaacct tcgctttgtc attcccccca aggccgtcga atggctggaa gccaacagct
      181 ggaattttag tgcgagcagc ggcagccagg tggcggcgga actttgtttc cgctacgaat
      241 acgactatcg ccggcggatg gcgatcaaga agatacccgg cgccggtgag gtctggatgg
      301 tctatgatag ccgtgaccgg ctggtcatga cccaggattc ggtactgcgc agccagcaaa
      361 aatggctctt cacacgctat gatgtcctga accggccgga ctctaccggg ctgatcaccg
      421 acccggccaa ttacaataac ctggcttatc accagaatct ggcgggatcc agtactgctt
      481 atccgaatct ttcttcctac actactgaga tcctgaccgg gacgttctat gatggctatt
      541 cgggtatcag cggggtctcc ggcctgccat cctccatggc cactggcgtc gccggcaaca
      601 gcagttattt tgtcaccaac tacaacgccg cacccatcta tgccgtgccc ataacggcct
      661 accctattac taggggagaa gtgacaggca ccatgatcaa ggtggttgga actgccaacc
      721 agtaccttta cagcgagagt ttttatgacg accgggcacg ggccatacag gcgcaaagca
      781 tcaattatac gggtggtgcc gacacggtta ctactcagta cgacttctct ggtaagcctc
      841 tgcgtacgct gctgggccag gccaagccga ccaacacagc ccaataccat cgggtcttga
      901 caaaaatgaa ctacgacgct ggttttcgcg ttaccagcat ctggaagaat atcgatgggg
      961 cagcggccga tcaattgatc gacagcatgc agtacaatga actagcccaa ccccgggcca
     1021 aatacctggg caataacctc gatagcctca tttacgatta caatatccgg ggctggatga
     1081 cggggatcaa caagggctat gtcggcggaa cgacaagtca ctattttgga atggaactgg
     1141 gctatgacaa ggcctccagc gtcgccggaa caagctatac tactccatta tataacggca
     1201 atatcgccgg cacggtctgg aaaagcgccg gtgacggcat cgaccggaaa tatgactttt
     1261 cctacgataa tgtcaaccgg ctcactggag cggcctacct ggacaaccac agtggcagtg
     1321 gctgggaccg atcggcgatg gactatacgg tcgacagcct tagctatgac gccaatggga
     1381 atattctatc catgaaccaa cacggttttg tcatgggcaa tcccaccggg gacatcgatc
     1441 aactactgta ttcctatcaa acaaacagca ataagctgtc acaggtcacc gacgccatga
     1501 acaaccccaa cagcaacctg ggcgacttcc attattcggg cacaaagcag gcctatgatt
     1561 atacctacga cggcaatggg aatttgtcgc tggacaataa caagaacatc gaccatatcg
     1621 tgtacaacta tctcaacctg ccgcaacagg tgcacatgaa caccaaggga aatatcctat
     1681 atacctatga tgcggccggc aataaactgc aaaagcaggt gatcgacagt acctccaatg
     1741 tcgcaaccac cacgctctac ctggatggct ttcagtacca acgacggacg cctctttcga
     1801 caccttcgat cggcgttgac accttgcaat tcatggggca cgaagagggc agggcacgct
     1861 gggccttcca taaatacacc aacggagact ccgcctatgc ctgggaatat gactttatgg
     1921 aaaaggacca tctcggcaat acccgggtcc tactaaccca gcaaaaggac accgcgcagt
     1981 acctggccac gatggagcca cagtactact cgaccgagaa tgccctgttt tacaatatgt
     2041 atactcaggc tcctataccc acggattttc cggcggagcc caatggcccg gtcaatccta
     2101 atcagtatgc tgtcggcctg gatggctaca cctggacggt gggacccgcc attgtcctaa
     2161 aggtgatgag cggcgacagc ctggctattg cctgcaattc gttttatcgg gggggtggga
     2221 ctgtcccatc ccccaagaac tcttttcccg aggcgctcaa ttccctggcg gaaggtctcg
     2281 ttgccatgac gggtggtacg catggcagtg tgagcgatct gaaagccgcc aactctcccg
     2341 tcgcagaggc gcttacctcc ttcctgacta cccaggattc caacaccacc aaccgtccga
     2401 aggcctatct caactggatg ttacttgata accagtttaa ttatgtgagc gacaatgggc
     2461 aaagcggcgc catcatcgcc agcaatccta acacgttgaa tacgatagcc accaccgttg
     2521 gtataaaaca cagcggattt ttgtatattt gggtgagcag cgagaccccg ttctggcaaa
     2581 tgtacttcga taacctgtcg gttcagcata tcgccgggcc gatggtggag gagaatcact
     2641 attatccctt tggtcttaca atggcgggca tttctgacaa agccatcaag acccaatacg
     2701 ctgaaaataa atacagatac aacggaaagg agttgcaaaa tcaggaattc aatgatggaa
     2761 gcgggtttga ggagtatgat tatggcgcca ggatgatgga tccgcagttg gggatatggc
     2821 atgggattga tcccttggct gataaatcga gaaggtggtc gccctatgtc tatgcttaca
     2881 acaacccgat acgatttatt gatccagatg gaatggaagg tgagacatat gggtatggtc
     2941 atactgatac agaaagcgaa tactggaact ctgaaaatta tgagggcggc ctgagtgtcc
     3001 aaacaagcaa aggccaaata tctgctggag ggggcaccag tggaggcaaa aatggcggag
     3061 gtggcaagaa aaagaatgaa aaagaaaagt ccaccgttca aaagtatgat ggctctggtt
     3121 taattcccac tcctcaaaaa gataagatta aattgccaga gtcaattgag cacttaaagg
     3181 agtttatgga aaagacaaaa ctgggtgaaa aaatcgaaac ggcgggcaca atgaatgagt
     3241 ggtcagggaa attaagcaaa attggcggtt atgacaaatt gagtgaacat ttggaaatta
     3301 taggaaaagg attctctctt gtgaacgcag ttaatcatta tttggaagga aagtatttcg
     3361 agggaactct tgatttagca agcatgacaa aactaagccc ttatgttttt gtatatgaaa
     3421 ccgttaacac agttgtcact tcgaaattca cattaaacca agcggcatat gacgccgatc
     3481 aaacggctaa ccaattctta aatgcggcac atgccgctag agttagcggc gatgatgcag
     3541 cagcagcgga gtattggaat gaggctgcca gatatgaagc aattaggaat cggattctta
     3601 gccaaaaaaa caaagacaaa taggatgaaa agcatctttg tattgttgtt gcctcttttg
     3661 tttatacagt gccgaagaca ctcgaccgac agagattctg gtgctggaac tactctagat
     3721 gttggcaaaa aaaggattga ttcactaaaa ga
//
LOCUS       DATGFG010000280         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_947303, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000280 DATGFG010000000
VERSION     DATGFG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947303"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="VKQ52_05175"
     CDS             <1..1514
                     /locus_tag="VKQ52_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ86604.1"
                     /translation="VGDYFLFRGKPTGFIPTEDDGRVYISFEIPEAGSTTRAIAVLDS
                     IQHMAQTVPGVLHTAALGGLNILTFGSKSNAGSIFVQFKPWDERKKKSEQLYGIVAEM
                     QKRFSTIKEARIVVIPPPAIPGLGNAGGFSIQIEQRQSNDDIKTFERVVNQFVAEANK
                     QPEIGQAFTFFSAHTPGFQVDVDREKAKKLGLRLDDVYNTMQAYMGSTYINQLTIYGR
                     NFYVVAQADTNYRTDIHNLDKYYVKNSNGEMIPLSTIVTTRMTESAPLISHFNIYRSA
                     EIDGGAKPGFSSGQAIEALQRVAAKVLPEGYGYDWSGLSLQEIKSGNSASYIFALSIV
                     FVFLFLAALYESWAVPFSVLLAVPVGVFGAILTLTLIPRLTNNVYAQIGLITLIGLSA
                     KNAILIVEFAKERVDRGMGVVQATLDAVRLRLRPILMTSIAFLLGVLPLAVATGASSV
                     ARNTIGWTVLGGMFAATSLAIFIVPVLFVVITRLTYGKRKLEDLRNKEYNLAE"
     gene            complement(1604..>2686)
                     /locus_tag="VKQ52_05180"
     CDS             complement(1604..>2686)
                     /locus_tag="VKQ52_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ86605.1"
                     /translation="LIIGTAVVYRQLSYIQHKKLGYDRDQVVIVQDTYFLGNNQRFFK
                     DVLTRDPRVASVSTSGYLPAGASYNNNFLVYADNSSSQLVNTLRYEVDEDYIPTLGME
                     METGRNFSKAFGSDSLGMIINETAARNFGWGDKALGHTLTRPNNDNTSTTYHIIGVVK
                     DFHFRSLRERITPLVMSLGSNQGSSFIVRTKTRDVAGLLGAMQKEWTDLKAGAPFSYS
                     FLDQRFQDTYMAEQNIAHILGIFAGLTIFVACLGLFGLATFTAEQRTKEIGIRKVLGA
                     NTASVVALLSKDFLRMVGLAFLIAAPIAWWVMNKWLQDFAYRISIGWWVFVLAAGLAM
                     LITLVTVSFRAIRAASANPVNSLRSE"
BASE COUNT          567 a          788 c          784 g          547 t
ORIGIN      
        1 gcgtggggga ctatttcctg ttccgcggca agccgacggg ctttatccct accgaagacg
       61 atggccgtgt ctatatctcc ttcgagatcc ccgaggccgg atccaccacc cgcgctatcg
      121 ccgtcctgga cagtatccag catatggcgc agactgtccc cggcgttctc catacggccg
      181 ccctgggtgg cctgaacatc ctcaccttcg gttcgaaatc gaatgccggc agtatcttcg
      241 tccagttcaa accctgggat gagcggaaga agaagagcga acagctttac ggcatcgtcg
      301 ccgagatgca aaagcggttc tcgaccatca aggaagcgcg tatcgtcgtc attccacccc
      361 cggccattcc cgggttgggt aacgccggcg gtttcagcat ccagatcgaa caacggcaat
      421 cgaacgacga tataaaaacc ttcgaacggg tagtgaacca gttcgtcgcc gaggcgaaca
      481 aacaacccga gatcggtcag gcctttacct tcttcagcgc ccataccccg ggcttccagg
      541 tggatgtgga ccgcgaaaag gccaagaagc tcggcctgcg gctcgacgat gtgtacaata
      601 ccatgcaggc ctatatgggc agcacgtata tcaatcagct gaccatctat ggccgcaact
      661 tttatgtcgt cgcccaggcg gataccaact accgcaccga tatccataat ctcgataaat
      721 actatgtgaa gaatagcaat ggcgagatga tcccgctcag tacgatcgtc accacccgga
      781 tgacggagag cgcgccgctg atctcccact tcaatatcta tcgcagcgcc gagatcgatg
      841 gtggcgccaa gccgggattc agcagcggcc aggccatcga ggccctgcag cgggtggccg
      901 ccaaagttct gccggaagga tatggctatg actggtcggg tctcagcctg caggagatca
      961 agtcgggtaa cagtgcgtcc tatatcttcg ccctgtccat cgtcttcgtg ttcctcttcc
     1021 tggcggccct gtatgagagc tgggcggtac ccttctccgt gctgctggcc gtgcccgtcg
     1081 gcgtcttcgg ggcgatcctg acgctgacgc tgataccccg gctcaccaat aacgtatatg
     1141 cgcagatcgg tctcatcacg ctgatcgggt tgtcggccaa gaacgccatc ctgatcgtgg
     1201 aatttgcaaa agaacgggtc gatcgcggaa tgggtgtcgt ccaggcgacg ctggacgcgg
     1261 tgcggctgcg gcttcgaccg atcctgatga cctccatcgc cttcctgctc ggggtgttgc
     1321 ccctggcggt ggccaccggt gcgtcatcgg tcgcccgtaa caccatcgga tggacggtat
     1381 tgggcgggat gttcgccgcc accagcctgg cgatctttat cgtccccgtg ctgttcgtgg
     1441 tgataacccg gctgacctat gggaagcgga aactggaaga tctgagaaat aaggaatata
     1501 atctggcaga gtaaacagag tggcgttgat caaaagtatc ggcctgccct tccggggcag
     1561 gccgatctgt tttaggacta ttcgggaggg ctatccggaa cggctattcg gagcgaaggc
     1621 tgttgaccgg atttgcgctc gctgcccgga tggcgcggaa gctgaccgtc accagcgtga
     1681 tcagcatggc gaggcccgcg gccaggacaa agacccacca gccgatggag atgcgatagg
     1741 caaaatcctg cagccatttg ttcatcaccc accaggcgat cggcgccgcg atcagaaagg
     1801 cgagccccac catccgcagg aaatcctttg acaggagggc gacgacactg gcggtattgg
     1861 cccccagcac cttccggata ccgatctcct tcgtccgctg ctccgcggtg aaggtggcca
     1921 ggccaaacaa gcccagacag gccacaaaga tggtcagccc ggcgaagatg ccgaggatat
     1981 gcgcgatgtt ctgttcagcc atgtaggtgt cctggaaccg ctggtccagg aagctatagg
     2041 aaaagggcgc cccggccttc agatcggtcc attccttttg catggcgccc aacaggccgg
     2101 cgacatcccg ggtctttgtc cgcacgataa aggaagaccc ctgattgctg ccaagggaca
     2161 tcaccagggg cgtgatccgc tcgcgcaggc tccggaaatg gaagtctttg accacgccga
     2221 tgatatgata ggttgtgctg gtgttgtcat tgttcggccg ggtgagggta tgaccgagcg
     2281 ccttatcccc ccagccgaaa ttgcgggcgg ccgtctcatt gatgatcatg ccgaggctgt
     2341 ccgagccaaa ggcctttgag aaattacgcc ctgtctccat ctccatcccc agggtaggga
     2401 tatagtcctc atccacctca taccgcaggg tgttgaccaa ttggctgctg ctgttgtcgg
     2461 catagaccag aaaattatta ttatacgagg cgccggccgg caggtagccg gaggtgctga
     2521 cactggctac ccggggatcc cgggtgagga catccttgaa gaaccgctgg ttgtttccca
     2581 ggaaataggt atcctggacg atcaccacct ggtcgcgatc atagcccagc ttcttatgct
     2641 ggatatagga gagctgccgg tatacgacgg ccgtgccgat gatcag
//
LOCUS       DATGFG010000281         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_876231, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000281 DATGFG010000000
VERSION     DATGFG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876231"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VKQ52_05185"
     CDS             <1..489
                     /locus_tag="VKQ52_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ86606.1"
                     /translation="CLKIAFRYVNTYEQATEVTHDAFLKMFRNFSRFEVRDKEKVEML
                     LIGWIRRVVINAAIDYMRREMNNTQTYAIPEHVWEHKDDGQSADNSVLYKELIALVKE
                     LPPAYRIVFNLHVIEGYSHPEIAKMLGITTGTSKSNLSKARVHLQKALTAENSERILC
                     QI"
     gene            477..1982
                     /locus_tag="VKQ52_05190"
     CDS             477..1982
                     /locus_tag="VKQ52_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ86607.1"
                     /translation="MSNLKDTDIDDLLRRASDDYPLRTDSSNWDRMAAALEEDPNPDP
                     AYIFATQDNRRSRRFLWLFLLLPLAGAGYYTWHQSGHGTFANGHKNVATKNVATDNGT
                     RPDNGTHTDNGTRTDNAPAAGQSNGAGNNGAPAATTPAATNHNNGAGATTPATDANIA
                     PNQPANRPSVTHNNPATVKSTTPVTSTNSATTSTISAHPLTATAGNNRSTSTTPARST
                     RAVGHDRFAAGRTTYPDATNTANTNPANANRQTPSTPIASAFTLRYSDIDQQRARIAG
                     AWNLSVDVKAPAIQKDTTKKKASPKAKTSYFYAGILGAPDFSTVKFQKTKGAGATFGI
                     LIGYAFNDRWSIETGAYLDKKRYYTDGEYFSKDKINLPTNIDLLNVDGACYMWEIPLN
                     VRYTFNQGAKTRWFATAGLSTYLMNRENYSYAAKYYNGTQTWTSTKDLKTPSQYPFSI
                     IGLSAGFEQRLGKLGNLRVEPYVRIPLGGIGTGSLPIMSAGVNVGITRRLW"
     gene            2099..>2290
                     /locus_tag="VKQ52_05195"
     CDS             2099..>2290
                     /locus_tag="VKQ52_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86608.1"
                     /translation="MRKKRAILWIGIPLLLIIIGAAWAWHLYDKLHESAAGESAAVTI
                     NADILYHQYQADEHAADQKF"
BASE COUNT          623 a          751 c          529 g          387 t
ORIGIN      
        1 tgtctcaaaa ttgccttccg gtatgtcaat acctacgaac aggcaaccga agtgacccac
       61 gacgccttcc tgaaaatgtt caggaatttc agccgtttcg aagtgcggga taaggaaaaa
      121 gtggagatgc tgctcatcgg ctggatcagg agggtcgtga tcaatgcggc catcgattat
      181 atgcggaggg aaatgaacaa tacccaaacc tatgcgatcc cggaacacgt gtgggagcat
      241 aaggacgacg gacagtccgc cgacaactcc gtcctctata aggagctgat cgcgctggtg
      301 aaagaattac caccggccta tcgcatcgtg ttcaacctgc acgtcatcga aggatattcg
      361 catcccgaga tcgcaaaaat gctggggatc acgacaggaa cctctaaatc taacctatcc
      421 aaggcccgcg ttcatttaca aaaggctttg accgcagaaa acagtgaaag aatattatgt
      481 caaatctaaa ggacaccgac attgacgatc ttctccggcg ggcgtccgat gactacccgc
      541 tgaggacaga cagttccaac tgggacagga tggccgctgc cctggaagaa gatccaaacc
      601 ccgacccggc ctacatcttc gccacccagg acaatcggcg gagcaggaga ttcctttggc
      661 tgttcctcct gctgcccctg gccggcgcag gctactatac ctggcaccaa tccggccacg
      721 gcaccttcgc caatggccac aaaaacgtcg ccaccaaaaa cgtcgccacc gacaacggca
      781 cccgccccga caacggcacc cacactgaca acggcacccg cactgacaat gcccccgccg
      841 ccggccagag caacggcgcc ggcaataacg gcgccccggc tgccaccaca cccgccgcca
      901 caaaccacaa caacggcgcc ggcgctacca cgcccgccac ggacgccaac atcgccccca
      961 accaaccggc gaaccggccc tccgttaccc ataacaaccc cgccactgtt aaatcaacaa
     1021 ccccggtaac ctcgacaaac tcagctacaa catccaccat atcggcacac cccctaacgg
     1081 caacggccgg caacaacagg tcaacctcca ccactcccgc caggagcacc cgcgccgtcg
     1141 gccatgaccg attcgccgcg ggccgcacca cctatcctga cgcaaccaat actgccaata
     1201 ccaacccggc caacgcgaac cgccaaaccc catccacccc catcgcctcg gccttcaccc
     1261 tgcggtactc cgacatcgac cagcaacgcg cgcgcatcgc cggcgcctgg aacctgtccg
     1321 tcgacgtcaa agcacccgcc atacaaaaag atactacgaa gaaaaaggca tctcccaagg
     1381 ctaaaacctc gtatttctat gcaggtatcc tcggcgcccc cgacttcagc acggtaaaat
     1441 tccaaaaaac aaagggcgcc ggcgccacct tcggcatcct gataggctat gccttcaacg
     1501 accgctggtc catcgaaacc ggcgcctatc tcgacaagaa aagatattac accgacggag
     1561 aatacttcag caaggacaaa ataaacctgc cgaccaacat cgatctgctt aacgtcgacg
     1621 gcgcctgcta tatgtgggag atcccgctca acgtccgcta taccttcaac cagggcgcaa
     1681 agacccgctg gtttgccacc gcaggtctgt ccacctatct gatgaatcgc gagaactata
     1741 gctatgcggc taaatattac aacggcaccc agacctggac cagcaccaag gatctgaaaa
     1801 cgccttcgca atatccgttt tctattattg gcctgagtgc aggtttcgaa caacgcctgg
     1861 gaaaactggg caacctgcgg gttgaaccct acgtaaggat ccccctggga ggcatcggca
     1921 caggcagtct gccgatcatg agcgccggtg tcaatgtggg tatcacgaga cgactctggt
     1981 aggaataaac aagaccggat gaaatccggt ccgtacacga agtgtacaga cgttaggggt
     2041 attgaggcaa aaaatatttt gacgacgatt ttttgccgaa atttttaaca gtaaaaacat
     2101 gcgtaaaaaa agagccattt tatggatagg gatccccttg ctcctgatca tcatcggcgc
     2161 ggcctgggcc tggcatctct acgacaagct acacgaaagc gccgccggcg aatccgccgc
     2221 cgtgaccatc aacgccgata tcctctacca tcaataccag gcggatgaac acgccgccga
     2281 tcagaaattc
//
LOCUS       DATGFG010000282         6044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1625597, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000282 DATGFG010000000
VERSION     DATGFG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625597"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VKQ52_05200"
     CDS             complement(<1..299)
                     /locus_tag="VKQ52_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86609.1"
                     /translation="MADIKPDEISAILRQQLGNSNVTAELEEVGTVLQVGDGIARVYG
                     LSNVSAGELVEFENGVRAIALNLEEDNVGVVLMGESGDIKEGATVRRTGKIASIRV"
     gene            complement(431..997)
                     /gene="atpH"
                     /locus_tag="VKQ52_05205"
                     /pseudo
     CDS             complement(431..997)
                     /gene="atpH"
                     /locus_tag="VKQ52_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
     gene            complement(1001..1495)
                     /gene="atpF"
                     /locus_tag="VKQ52_05210"
     CDS             complement(1001..1495)
                     /gene="atpF"
                     /locus_tag="VKQ52_05210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HLZ86610.1"
                     /translation="MDLLTPDLGLFVWNIVIFLLLLIILRKLAWKPILKSLNEREKGI
                     SDALASAEKIKVEMAQMKNENEALLAKAREERAQLLKEARETKDKMINEAKEMAKVEA
                     SKIIADAQVAIEQQKMAALTEVKNEVGTLVVDIAEKILRRQLENKSEQETYIRQLSNE
                     AKLN"
     gene            complement(1580..1828)
                     /gene="atpE"
                     /locus_tag="VKQ52_05215"
     CDS             complement(1580..1828)
                     /gene="atpE"
                     /locus_tag="VKQ52_05215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HLZ86611.1"
                     /translation="MTLMTTLLADVVQPGYGALGAGLAAIGAGIGIGQIGKGAVDSIA
                     RQPEASNDIRANMILAAALVEGVALLAAVAGLLAVFQK"
     gene            complement(1861..2955)
                     /gene="atpB"
                     /locus_tag="VKQ52_05220"
     CDS             complement(1861..2955)
                     /gene="atpB"
                     /locus_tag="VKQ52_05220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLZ86612.1"
                     /translation="MSVVHAAPGDQEGTGKQEARKLDPAKIILEHVSDAHEFHFFTFN
                     KKPVTIPLPVILYSPVKGWSVFLSSAFEHGEKLVNGYRLLTEEYVEEKKLDGKIYKPG
                     KIWAVNEAGDVDPSVKVYDLSLGRNAVQAMISALILLLIMPGIAAKYRRGEGVKTAPT
                     GFQNLIEPVITFVRDEVGKSNLGDKYEKYMPFLLTVFFFILINNLLGLIPGTANVTGN
                     IAFTGALGILAFIAIMFSTGKHYWAHIVNPPVPGPIKVIMWPVEIMGIFTKPFALIIR
                     LFANMISGHIIILSFICLIFIFGAMNTALGWGTSPFFVLLSIFIYLIEVLVAFIQAFI
                     FANLTAVFISQAFEGGHEEDHGHGHETVIA"
     gene            complement(3186..3890)
                     /locus_tag="VKQ52_05225"
     CDS             complement(3186..3890)
                     /locus_tag="VKQ52_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ86613.1"
                     /translation="MKLNCIIVDDEYLAIRILEDYCLRVPSVTVLRSFKDPLLAMEFL
                     RKNVVDLLFLDIQMPRLSGVDMAAAFADIDGKRPRVVFTTAAGDFAVKAFDLEALDYL
                     VKPISFQRFEKAIRKAEETLSSPDLTTPQDFLLLKSDHRTLRVALDEIILIEGLNEYV
                     KVHTPDKRIVTLAALKDLEARLPGSRFVRIHKSYIVSLSHIRWWNTSEVEITGGMRLP
                     IGRVYKEQFLNRLPDR"
     gene            complement(3887..4930)
                     /locus_tag="VKQ52_05230"
     CDS             complement(3887..4930)
                     /locus_tag="VKQ52_05230"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLZ86614.1"
                     /translation="MKYRKTAFNFALYAQIVFWVVLFLFLLFSSMGAGARRPLVAASL
                     LLLCHLFNFYPVFAWLTPRYFEKRRYAAFLSGLVLVFLVLTPFRHWVESRFVHRPFLV
                     LRGRSLSGLILFSELSVAGFAFLLRMALDNFASRQRADELEKSHLVAELKFLKSQMNP
                     HFLFNTINNLYALALLGSEKTPEALLRLSELLRYLLYECNHRVPFYKELTAIESYIAL
                     LQLRYEEPLDITVDTRVSTTDMPVEPMLLIPLLENAFKHSGIGVTPGAFIRVQLREDR
                     GLLLGHFINSRLDDRDRGEARTSLPGDNHSGIGLQNIRKRLEMIQPYRCEENLVILHT
                     PEVFEVFIKIQGK"
     gene            5084..5986
                     /locus_tag="VKQ52_05235"
     CDS             5084..5986
                     /locus_tag="VKQ52_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86615.1"
                     /translation="MKLTRIILIASAGSVLFTGLILSACNKSNSSGSSSTASESDAVT
                     MSASSATADNQYNDVLNVGLQVGAGGSTTIDKADGGVLTGNRKEVDGPGGATPCALLS
                     ASITDSTTYPVTVSIDFGTGCTGADGITRKGKITYVFSGRIYTPGTTVAVSFTGYSVN
                     GYQLAGNYSITNTSAGNGISFTWKVVGGQITFPDGTAYTYAGTRNVAQTAGIGTLTLL
                     DDSYSITGSHSYGSSTGKTLTDSITTPLVWPVTCDHLVSGVIGFTYTYSGMSLKGTLD
                     YGSGACDDSAMIKVGATTKTVVLP"
BASE COUNT         1420 a         1723 c         1577 g         1324 t
ORIGIN      
        1 acccggatag aagcgatctt accggtgcgg cgaacggttg caccttcctt gatgtcgccg
       61 ctctccccca tcaataccac gccgacattg tcttcttcca ggttgagggc gatggctctg
      121 acgccatttt cgaattccac cagttcaccg gcgctgacat tgctgagtcc atacacccgg
      181 gcgataccat cccctacctg caggacggtg cccacctctt ccaactccgc ggtaacgttg
      241 gaattgccca attgctgacg caatatcgct gaaatttcat ccggtttgat gtctgccata
      301 attgtattca gtttgaagtt tgtttgttga atgtgcgcgg ctaggtctac tgatcaactt
      361 agccctatcc gcgcaaacag ctaatgtctg accttcggtc gagccgcttc cagcggctca
      421 atggtctctt ttacctgatc ttatacataa aatcgttgtt ctggaactgc ttgcggatat
      481 tgttcagctc gaaagccacg ctgctgtcca gccgctcgtc accgatctcg aggataaaac
      541 cgccgataag ctcctgccgc acctccgtat ccaactccac ctactttaat tggcgttcgg
      601 tctgcacctt ccgcaggata gcctgcttca catcgtcgct caccggaacc gccgtcgtga
      661 gcttgacggt ggaaatcccc ttatacgtct tgtattgctc tacaaaggca aacgcaatct
      721 ccgggagaag tccttcccgc tctttattca gcaatagctt tatgaacgcg gcagtgagcg
      781 gaccgatctt gcccgcgaac accatctcca ggatcttgcc cttcttgtcg gccttcacga
      841 cggggctgcg caggagattc accagctccg cactgtcgcg gcagccctgc ttcaggagca
      901 gcatatcttt attcacatcg tccgcctgtc ctcgctcgag ggaaaggtcc aggatcgact
      961 tggcgtaacg cgccgctaaa cggggattgg gcatcatagg ttagttgagt ttcgcttcgt
     1021 ttgacaattg acggatatag gtctcctgtt cactcttgtt ctccagttgg cggcgcagga
     1081 tcttttccgc gatatccact accagcgtac ccacctcgtt cttgacctcc gtaagcgcgg
     1141 ccatcttctg ttgctcgata gccacctggg catcggcgat gatcttgctc gcttccacct
     1201 tggccatttc ctttgcctcg ttgatcatct tatcctttgt ctcccgggct tccttcagca
     1261 actgggcgcg ttcctcacgg gccttggcca gcaacgcctc attctcgttc ttcatctggg
     1321 ccatttccac cttgatcttc tcggccgacg ccagcgcatc ggaaatgccc ttttctcttt
     1381 cgttaagaga cttcaatatc ggcttccacg ccagctttct caggatgatc agcagcaaca
     1441 ggaagatgac gatattccaa acaaataagc ctaagtcggg agttaagaga tccatagtat
     1501 aatcttaatg tgttttttta agagaaaaat actgcatcct cggccctgtg cgtcggatgc
     1561 agtacgcttt ttacgtgcct tacttctgga atacagcgag cagaccggca acggcagcca
     1621 gaagggcaac cccttcgacc agggccgcag ccaggatcat gtttgcacga atgtcgttcg
     1681 aagcttccgg ctgacgggca atggaatcaa cagcgccctt accgatctgg ccgataccga
     1741 taccggcgcc gatagcagcg aggcctgcgc caagtgcgcc ataaccaggc tgaaccacat
     1801 cagccagtaa agttgtcatc aacgtcatct tatttggttt tataattaaa aaagagataa
     1861 ttaggcgatt actgtttcgt ggccatgacc atgatcctct tcatgccccc cttcaaaggc
     1921 ctgggaaatg aacacggccg tcaggttggc aaaaataaat gcctgtatga aggcgaccag
     1981 gacttcgatc aggtagataa agatcgaaag caggacgaag aacggtgaag ttccccagcc
     2041 cagcgcggtg ttcatggcgc cgaagataaa gatcaggcag ataaagctga ggataatgat
     2101 atgtcccgag atcatattgg caaaaagacg gatgatcagg gcaaagggtt tggtaaagat
     2161 ccccatgatc tccaccggcc acatgatgac cttgatggga cccggaacgg gcggattgac
     2221 gatatgcgcc cagtaatgct tgcccgtgct gaacatgatg gcaataaacg ccaggatacc
     2281 caacgccccg gtaaaagcga tattcccggt aacattggcc gtacccggga tcagcccgag
     2341 caggttgttg atcaggataa agaagaagac ggtaagcaga aagggcatgt atttctcata
     2401 tttgtccccg agattgctct tgccgacctc gtcacggaca aaggtgatca ccggctcgat
     2461 aagattctga aaacccgtgg gcgccgtctt caccccttcc cccctccggt acttcgcggc
     2521 aatgccgggc atgatcagga gcaggataag cgcggaaatc atcgcctgaa cggcattccg
     2581 gcccagtgaa agatcatata ccttaacgga aggatccaca tcgccggctt cgttaacggc
     2641 ccagatcttc cccggcttat agatcttccc atccagtttc ttctcctcga catactcctc
     2701 tgtcagcagc cggtagccat tgacgagctt ctctccatgt tcaaaagcgg atgataggaa
     2761 taccgaccag cccttcaccg gggaatacag gatcaccggc agggggatcg tcaccggctt
     2821 cttattgaac gtgaaaaaat ggaattcgtg ggcgtcggaa acgtgctcca ggatgatctt
     2881 ggcagggtcc agcttccgtg cttcctgctt ccccgtccct tcctggtcac cgggagccgc
     2941 gtgtactacg gacacattaa aaaaaaacag cacaaccacg ctgaaaaccg cgaccaatat
     3001 agatttgaag cattttgcca ccataccgtc ctgaaatttg ccgcaaagat acgggcggag
     3061 ggtcagaata tgaaaataag attaaaaaaa aggccgttgt aagaggggcg gctccacgga
     3121 ggggtaggga tgattcagtt tgttgataat caacatcaat catgtacagt cgaagccgtt
     3181 atccttcacc ggtcgggaag ccggttcagg aattgttcct tgtatacccg gccgatgggc
     3241 aaccgcatcc ctccggtgat ctccacttcc gacgtgttcc accagcggat atgggacagg
     3301 gatacgatat aggatttgtg tatccggaca aaccgggatc ccggtagcct cgcctccagg
     3361 tctttgagcg cagccagcgt cacgatccgc ttgtccggcg tgtggacctt gacgtattca
     3421 ttgagaccct ctataaggat gatctcgtcg agggccacgc ggagggttcg gtggtcgctt
     3481 ttcagcagca ggaagtcctg cggagtggtg aggtccgggg agctgagcgt ctcttccgcc
     3541 ttgcggatgg ccttttcaaa tcgctggaag gaaatgggtt tgacgagata atcaagggct
     3601 tccaggtcga acgcctttac ggcaaagtcc ccggctgccg tggtaaacac cacccgcggc
     3661 cgctttccat cgatgtccgc gaaggcggcc gccatatcga ccccggacaa ccgcggcatc
     3721 tggatatcga ggaacagcag gtctaccaca ttcttccgga ggaactccat ggccagcagg
     3781 gggtctttga aggaccgcaa caccgtgacc gagggtacgc gcagacaata gtcttccagg
     3841 atacggatag ccaggtattc atcatccacg atgatacagt tcagcttcat tttccctgaa
     3901 tttttataaa gacctcgaat acttccgggg tgtggagaat aacaaggttt tcttcacacc
     3961 ggtaaggctg gatcatctcc agccgtttgc ggatgttctg caggccgatg cccgaatggt
     4021 tgtcgccggg aaggctggtc cgtgcttcgc cgcgatcacg atcatccaac cgggaattga
     4081 tgaaatgccc cagcaacagc ccccggtcct cccggagctg gacccggata aaggccccgg
     4141 gcgtaacacc gatacccgag tgtttaaaag cattctccag cagcgggatc agcagcatgg
     4201 gttcgacagg catatcggta gtggagaccc gggtgtctac ggttatatcc aacggctctt
     4261 cataccggag ctgcagaagg gcgatatacg actctatcgc cgtcagctct ttataaaacg
     4321 gcacccggtg attgcactca tacaggagat accggagcag ttcggatagc cgcagcaggg
     4381 cctccggtgt cttctccgag ccgagaagcg caagagcata caggttgttg atcgtattga
     4441 aaaggaaatg gggattcatc tgagacttga ggaatttcaa ttcggccacc aggtggcttt
     4501 tttccagttc atcggcgcgc tgccggctgg caaaattgtc cagcgccatc cgtaacagga
     4561 aggcgaaacc ggccaccgaa agctcggaga agaggataag ccccgagagg ctccgtccgc
     4621 ggagcaccag gaagggccgg tgaacaaacc gcgattcgac ccagtgacga aaaggcgtga
     4681 gcaccaggaa taccaaaacc aggccggaaa gaaatgcggc atagcgtcgt ttctcgaaat
     4741 agcggggagt aagccaggca aacaccgggt agaaattgaa caggtggcag agcaaaagca
     4801 acgaggcggc taccaggggc cggcgggccc ccgcccccat ggaagaaaaa agcaggaaca
     4861 ggaacaggac gacccagaag acgatctggg catagagggc gaaattgaag gctgttttcc
     4921 ggtatttcat cagaagttac aacaaaaata ttccagatgg gcgagccgcc gttgcctgcc
     4981 ggccgtcgtt acctgaacgt ttccggtagt tgactgaaag gatagacgag ggtttaaacg
     5041 ccccgcgtta ttgccgtatc ctaaagataa aaaccaacca attatgaaac taaccaggat
     5101 catccttatc gcctcggcag gttccgtcct tttcacgggc cttattctct ccgcctgtaa
     5161 caaaagcaat agttcgggca gcagctccac ggcttcggaa tccgatgccg tcacgatgag
     5221 cgcctcgtcg gccaccgccg acaaccagta caacgacgtc ctcaatgtgg gcctgcaggt
     5281 cggcgccggc ggcagcacaa ccatcgacaa ggccgatggg ggcgttctca ccggcaaccg
     5341 caaagaggtg gacggacccg gcggagcgac cccctgtgcc ctcctctctg catccatcac
     5401 ggactccacc acctatcccg tcaccgtctc tatcgatttt ggaacgggtt gcaccggcgc
     5461 cgacggcatc acgcggaaag gcaagatcac ctatgtcttc tcaggcagga tctatactcc
     5521 cggcacgacc gtagccgtca gctttaccgg ttattcggtc aacggctatc agctggccgg
     5581 aaattactcg atcaccaata cgtccgccgg caacggcatc tccttcacct ggaaggtcgt
     5641 cggcggccag atcaccttcc ccgacggaac ggcgtatacc tatgccggaa cccggaatgt
     5701 cgcccagacc gcgggcatcg gcacccttac actgctggac gacagctatt ccatcaccgg
     5761 cagccatagc tatggcagca gcacggggaa aaccttgacc gatagcatca ccacgccgct
     5821 tgtctggccc gtcacctgcg atcatctcgt ctccggggtc atcggcttca cctataccta
     5881 cagtgggatg tccctgaagg gtacgctgga ctatggcagc ggcgcctgcg atgattcggc
     5941 catgataaaa gtgggagcta caacgaagac agtggtgttg ccttagcgga tttcttaacg
     6001 acgccctacg ctcatttacg acgccttacg ctctgcggcg gggc
//
LOCUS       DATGFG010000283         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_314299, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000283 DATGFG010000000
VERSION     DATGFG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314299"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="VKQ52_05240"
     CDS             <1..1619
                     /locus_tag="VKQ52_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLZ86616.1"
                     /translation="YGAAIGGRDYNSVDMSLRTLWEIYLPPFRAAEEAGVATFMNSFN
                     DLNGIPATGNAYLQRQVLKGKWKFTGFVVSDWGSVGEMINHGYAKDNKEAAREAVTAG
                     CDMDMESRSYINNLATLVREGKVSIQLIDEAVRRILAKKFELGLFDDPYRFCNAQREK
                     QQWDNPENLGIEQDMARKSIVLLKNDNQLLPLSKQTKTIALIGPLVRSVRENLGFWSY
                     DWPDDSNRIVSPWDGIRNKLGNGTRLLYAKGCGITDTSRAGFAEAVDIAQQADVVIMD
                     IGEARDMTGEAKSRSNIHLPGVQELLVQAIRATGKPMIVLISAGRPLVFNYTADHADA
                     ILYTWWLGTKAGNAIADVLFGDYNPSGKLPISFPRTEGQIPIYYNHFPTGRPAKNDSD
                     VNYVSAYIDLPNDPRYPFGYGLRYTHFRYSDLTLSAPSFKPGGRLTATVTLTNTGSFD
                     GRETVQWYIRDLVGSVVRPVKELKGFQQVFLKAGESKKISFMIDTDKLKFYNDKLQHI
                     YEPGEFRLFVGGDSRDLREIPFTLAIFDQN"
     gene            1623..2237
                     /locus_tag="VKQ52_05245"
     CDS             1623..2237
                     /locus_tag="VKQ52_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HLZ86617.1"
                     /translation="MRGIKNIIFDLGGVIINLDNQRTTDAFVALGLKNIKEYFGHGHA
                     ASFFKDYEVGTITDRQFIDAIRETAGLTASDQAIIDSWNALLLDFPPERIRLLSQLRR
                     SYRLFLFSNTNALHLAALQAIYTRTFGSGSLEDHFEKTYYSHLLKMRKPDPASYEYIL
                     RENGLNGPETLFVDDAIINVEGAEQAGLKGLYLRPGISLLDFQW"
     gene            complement(2274..2543)
                     /locus_tag="VKQ52_05250"
     CDS             complement(2274..2543)
                     /locus_tag="VKQ52_05250"
                     /inference="COORDINATES: protein motif:HMM:NF027444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HLZ86618.1"
                     /translation="MELSEVFFLAIVFYLLYRFVFNFLVPVARTTRHVRQQFRNMQEM
                     QDGMRQQMQDGMRQQQGPARPQQSQQRPAQRPPAGDYIDFEEVKE"
     gene            complement(2614..2931)
                     /locus_tag="VKQ52_05255"
     CDS             complement(2614..2931)
                     /locus_tag="VKQ52_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86619.1"
                     /translation="MSKLTKADRSLDTLDQEIRRLRAKAKQLEERIDENFSYFQQHSG
                     SMFVRSLLPRKIDGEELTGLRLVDTVLQNERLQNILLKLADILAEKLGDGLNWLTTRV
                     FKK"
     gene            complement(2924..3289)
                     /locus_tag="VKQ52_05260"
     CDS             complement(2924..3289)
                     /locus_tag="VKQ52_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86620.1"
                     /translation="MNQPDNLGSFFAENKTLLKEYLETRMEIYRLQSLRLFARSAGYF
                     AWILLSLFLAFLFLLFGGMMTAFWFSELFHSYFRGFGLVTLMILVVFVLLAIFRKALF
                     VNPVIHSVIQKSKEEEEHV"
     gene            3416..3883
                     /gene="rimP"
                     /locus_tag="VKQ52_05265"
     CDS             3416..3883
                     /gene="rimP"
                     /locus_tag="VKQ52_05265"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HLZ86621.1"
                     /translation="MINETVKAVESLVQALLSGDQGYFLVDVRIKPTNNVKVYIDGDQ
                     GISIEKCVQYNRALYKKLEESGLFPTGDFSLEVSSPGLDEPLKLLRQYRKNIGRQVEL
                     VMQDGSKKEGRLLEVSEDGIIVEEVKGKNKKKEVVNHTFLFDNIKTTKIQVVF"
     gene            3902..>4203
                     /locus_tag="VKQ52_05270"
     CDS             3902..>4203
                     /locus_tag="VKQ52_05270"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HLZ86622.1"
                     /translation="MASINLIEAFQDFKEAENIDRPTMMKVVEDVFKTLLRKKYGSDE
                     NFDVIVNAEKGDLEIMRRRTIVEDGNVNDPLAEIAYTDATKIEPDYEVGEELYEEV"
BASE COUNT         1130 a         1129 c         1072 g          872 t
ORIGIN      
        1 cctacggcgc cgccatcggg ggcagggact acaactccgt cgatatgagc ctgcggaccc
       61 tctgggaaat atacctgcca cccttccggg ccgccgaaga ggcgggcgta gccaccttta
      121 tgaattcctt caacgacctg aacggcatcc ctgctaccgg taatgcctat ctgcaacgac
      181 aagtgctgaa aggaaaatgg aagttcaccg gttttgtcgt cagcgactgg ggtagcgtgg
      241 gagagatgat caaccatgga tatgcaaagg acaataagga ggcggcaagg gaagccgtca
      301 ccgccggctg cgatatggat atggaaagcc ggagctatat aaacaacctg gccaccctgg
      361 tccgcgaagg aaaggtgagc atccaactga tcgatgaggc cgtccggcgc atcctggcaa
      421 agaagttcga attgggactc ttcgatgacc cctatcggtt ttgcaatgcc cagcgggaaa
      481 agcaacagtg ggacaacccg gaaaacctcg ggatagaaca ggacatggcc aggaaaagca
      541 tcgtcctcct gaagaacgac aaccagctgc tccccctctc aaaacaaaca aagaccatcg
      601 ccctgatcgg cccgctggtc agatcggtaa gggagaacct cggtttctgg tcctatgact
      661 ggcccgacga ctccaaccgg atcgtctcgc cctgggacgg catccggaat aaactgggca
      721 acggaacccg tctgctctat gcaaaaggct gcggcatcac cgatacctcc cgcgccggtt
      781 tcgccgaggc cgtcgacatc gcgcaacagg cggacgtcgt catcatggac atcggcgagg
      841 caagggatat gacgggcgag gccaaaagcc gcagcaatat ccatctgccg ggagtacagg
      901 aactcctcgt ccaggccatc cgggccaccg gcaaaccgat gatcgtcctc atcagcgcgg
      961 gccgcccgct cgtcttcaac tacaccgccg atcatgccga cgccatcctc tatacctggt
     1021 ggctgggcac aaaagcgggt aacgccattg ccgatgtgct cttcggcgac tacaacccct
     1081 caggcaaact gccgatcagc tttccccgta cggaaggcca gatccccatc tactacaacc
     1141 acttcccgac gggccgcccc gcaaaaaatg acagtgatgt aaactatgtc tcagcctata
     1201 tcgatctgcc gaacgacccc cgctatccct tcggctatgg cctcagatat acccatttcc
     1261 gttatagcga cctcaccctt agcgcacctt ccttcaaacc cggcggcagg ttgacggcca
     1321 ccgtcaccct gaccaatacc ggcagcttcg acggccgcga gaccgtacaa tggtatatcc
     1381 gcgacctcgt gggaagcgtc gtgcgacccg taaaagaact caaaggattc caacaggtct
     1441 ttctaaaagc cggggaatcg aaaaagatca gctttatgat cgatacggat aagctaaaat
     1501 tctataacga caagttgcaa cacatctatg aacccggtga attccggctt tttgtcggtg
     1561 gtgactcgag agacctgcgg gaaataccgt tcaccctggc tatatttgac cagaattaac
     1621 ctatgcgggg aataaaaaac atcatcttcg acctcggcgg cgttatcatc aacctggaca
     1681 atcagcgtac gaccgacgcc tttgtcgccc ttggcctgaa aaatataaag gaatatttcg
     1741 gccatggcca cgccgcctcc ttcttcaagg actatgaagt gggaacgatc accgaccggc
     1801 aatttatcga tgccatccgg gagaccgcgg gcctcaccgc ctccgaccag gccatcatcg
     1861 acagctggaa cgccctcctg ctcgatttcc caccggaaag gatccggttg ctgtcacagc
     1921 tgaggagatc ctatcgcctc ttcctgttca gtaatacaaa cgccctccac ctggccgccc
     1981 tccaggccat ctataccagg accttcggct cgggctccct cgaagatcat ttcgaaaaga
     2041 cctattattc gcacctgctg aaaatgagaa aacccgaccc ggcatcctac gaatatatcc
     2101 ttcgcgagaa cgggctcaac gggccggaga ccctgtttgt cgatgacgct atcataaatg
     2161 tggaaggggc cgaacaggcg ggactcaaag ggctctatct gcgcccggga atatccttac
     2221 tggattttca atggtagtag tccaaagacg tgcaagacta ccgacgcgca agactactcc
     2281 ttcacttctt caaagtctat ataatccccc gccggcggcc gttgggcggg ccgctgctgg
     2341 gattgctgcg gccgggccgg accctgctgc tgccgcattc catcctgcat ctgttgccgc
     2401 atcccgtcct gcatctcctg catattcctg aactgctgac ggacatgacg ggtcgtacgg
     2461 gccaccggca ccaggaaatt aaaaacaaac cggtacaaca gatagaatac gatcgcgagg
     2521 aaaaatactt ctgataattc cataacaata ttacccgcta aggtagggaa aggttcggta
     2581 aaagggtgtt aaaccatgcc gaaatcgacc ccactatttt ttaaacactc tggttgtcaa
     2641 ccagttgaga ccgtcaccca atttctcggc caggatatcc gccaatttaa ggaggatgtt
     2701 ttgcagccgt tcattctgca ggaccgtgtc cacgagccgg agaccggtca gctcctcgcc
     2761 atcgatcttc cggggcagga gcgaccggac aaacatcgaa ccggagtgct gttggaaata
     2821 gctgaaattt tcgtcgatcc tttcttccag ttgctttgcc tttgcccgca aacgccggat
     2881 ctcctggtcc agggtatcca ggctgcggtc ggccttggta agtttagaca tgttcttcct
     2941 cctctttgga tttttggata acgctatgaa tgacaggatt tacgaaaagc gccttgcgaa
     3001 agatggccag cagcacaaag acgaccagga tcatcagggt caccaaccca aaacccctga
     3061 aataactgtg gaagagctcc gagaaccaga acgcggtcat catgccgcca aaaagcagga
     3121 agagaaaggc caggaataac gacaaaagga tccaggcaaa ataaccggcc gaccgggcaa
     3181 aaagacgaag actttgcagc cggtagatct ccattcgtgt ttcaaggtat tctttcagca
     3241 acgtcttatt ctcagcgaag aacgatccta aattatcggg ctgattcatt tagttctttt
     3301 cttaaaagta aaaatatttg gggaaagggg atgaattatc gtacctttgc agggattaaa
     3361 aagaaacaaa agaagggaac gggttcgttc ccttctgctt ttcttagcag gtattatgat
     3421 caatgaaaca gtgaaggcgg tagaaagttt ggtgcaggcc ctcctgtcgg gagatcaggg
     3481 atatttcctg gtggacgttc gcattaaacc cacaaataat gtaaaagtat atatcgatgg
     3541 ggatcagggt atttccatcg aaaaatgcgt ccagtacaac cgggcgttgt ataagaaact
     3601 ggaggagtcg ggtcttttcc ccacgggtga tttttccctc gaggtaagct ccccgggcct
     3661 ggacgaaccc ctgaaactgc tgcgccaata ccggaagaat atcggccggc aggtagagct
     3721 ggtgatgcag gacgggtcga aaaaggaagg ccgcctgctg gaagtgagcg aagacggcat
     3781 tatagtggaa gaggtaaagg gaaaaaataa gaaaaaagaa gtggtcaacc atactttttt
     3841 attcgataac ataaaaacaa caaaaattca agtagttttc tagagcttta aattcagtgt
     3901 tatggcaagt atcaatttga ttgaagcctt ccaggacttc aaggaagccg agaatatcga
     3961 tcgtcctact atgatgaaag tggtagagga tgtgttcaaa actttgttgc ggaagaaata
     4021 tggaagcgac gagaattttg acgtaattgt gaatgcggaa aagggtgacc tggagatcat
     4081 gcggcgccgg accatcgtcg aagacggaaa tgtgaacgac cccctggcgg aaattgccta
     4141 caccgacgca acaaagatag aacccgacta cgaagtgggg gaggaactct atgaagaagt
     4201 gga
//
LOCUS       DATGFG010000284         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_718399, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000284 DATGFG010000000
VERSION     DATGFG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718399"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VKQ52_05275"
     CDS             complement(<1..786)
                     /locus_tag="VKQ52_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86623.1"
                     /translation="MKFRFSVLFLFLLSAVIVHAQQAATGASVNLPLAAKKPLDPSVY
                     DKWESIGERMLSADGKWLVYTITPQEGDARLEIRGTASDYAKEIPRGTEATITEDGRF
                     VVFLIHPFFKDSREARIRKRTPDQSPKDSLGWLELGTDKLVKMPRVKSYKVAEKDGEW
                     LAYLLEKPLPPTAPQDSATRLRLLMSRADSLARVADSLHRQVAEVEAKGWKAAMPAGG
                     AGRGGGGRAAGEGVTGRGGGPGATGETPEEGTDLVLLNLHTGAE"
     gene            974..1822
                     /locus_tag="VKQ52_05280"
     CDS             974..1822
                     /locus_tag="VKQ52_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLZ86624.1"
                     /translation="MTTKLSLQKEYTLGGELKVRRMGFGAMRITGQGIWGPPKDKAEA
                     IRVLRRAVELGVNFIDNADSYGPHVSEDLIAEALYPYPKGLVIATKGGFLRTGPNQWH
                     VNSRPEHLREALESSLARLKQDQIHLYQLHRIDPTIPFQRTLEFLQGVQEEGLVKYIG
                     LSEVSVEEIKKAEEYVDIVSVQNKYSQDYRKWESVLHYCEETDKAFIPWNPINAGNLT
                     NMSAVDRVAGKIGATPHQVALAWLLYHSSNNLLIPGTSSVKHLEENIAAENIELTEEL
                     LEQLEK"
     gene            complement(1829..2311)
                     /locus_tag="VKQ52_05285"
     CDS             complement(1829..2311)
                     /locus_tag="VKQ52_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HLZ86625.1"
                     /translation="MANEFYELSAKTPQGRALNMADYQGKVVLIVNTATQCGFTPQFT
                     GLEKLYQQYKDQGLVVLGFPCNQFGAQEPLSNEVMEQTCLINHGVTFPLVEKIEVNGP
                     GTHPVFRYLKKKLGGLFGSRIKWNFTKFLVDRTGKPVKRYSPMTKPEAMEGEIKRLLG
                     "
     gene            complement(2691..3410)
                     /locus_tag="VKQ52_05290"
     CDS             complement(2691..3410)
                     /locus_tag="VKQ52_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86626.1"
                     /translation="MNEVIFETVLNDILDELKQANDRLRVMKESVKGLEERVAAFEQR
                     LTELQVTAPPADVGPVKEVVDGAMSVFRREQAASVERMTAIVEAQPKNVVRQWRWSIF
                     PDDDRAGNYQYLIKKLSMVALVGMPVACLFLLGVRWMDKSYAYRAGFEQASTASVGGP
                     GGKTAADAKTVMQGGKKGAPGGGTMAPGSLVAPRVRHGRGAGGDRRVPGVTGMGPGRA
                     VDTLNKKQIRINRDSLENPGD"
     gene            complement(3421..>3986)
                     /locus_tag="VKQ52_05295"
     CDS             complement(3421..>3986)
                     /locus_tag="VKQ52_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86627.1"
                     /translation="RIGFDRHTVTNQYMLRRMNSYCRESEQEYRLTPTPKIRWYRSKE
                     ERQEASQDQRVIRLGKVIGEALESVCQYDDFKKRLEEEGYRLYKTVRGIAFKDADNVV
                     LRGYETGYPWKEIEATLRKNELRQKQLLEQQEVLRQQELAQKQDGMPRQESAEKQGAQ
                     QDQESAQREEERLRQDRQLRHRHRHHF"
BASE COUNT          875 a         1391 c          891 g          829 t
ORIGIN      
        1 ttccgccccg gtgtgcaggt tcagcagcac gagatcggta ccctcctccg gtgtctcgcc
       61 cgtcgcgccg ggtccgccac cacgccccgt cacgccttca ccggctgccc gccccccacc
      121 gccacgacca gccccacccg ccggcatcgc cgccttccat cccttcgcct ctacctccgc
      181 cacctggcgg tgcagactgt ctgccacccg tgcaagacta tccgcccggc tcatcagcaa
      241 ccgaagccgc gtggcggagt cctgcggcgc cgtcggcggc agcggctttt ccaacaggta
      301 ggccagccac tccccatcct tctcggcaac cttataggat ttgacccgcg gcatcttgac
      361 aagcttgtcc gtccccagct ccagccaccc gagactgtct ttgggcgact gatccggcgt
      421 ccgcttgcgg atccgcgctt cccggctgtc tttaaagaaa gggtggataa ggaaaacgac
      481 aaatcggccg tcctcggtga tcgtcgcctc cgtcccgcgt ggtatctctt ttgcataatc
      541 actcgctgtc ccccggatct ccaggcgggc atcgccttcc tgcggggtga tggtatagac
      601 aagccatttc ccgtcggcgg aaagcatccg ctcgccgata ctctcccact tgtcatagac
      661 ggagggatcg agcggcttct ttgccgccaa aggcaggttg accgacgctc cggtggcagc
      721 ctgttgggca tggacgataa cagccgataa taggaaaagg aataagactg aaaaacggaa
      781 tttcatttgc agcattttgg ttgaagggat ttcgatccac ttcggccttc ggcctcgtgg
      841 acaaaccggc attgtccggc tatcgaatgt aaaataaacc cgggataccc ggtgaacaat
      901 ttacatgacc ggggcgttga cattccgcca ttcgccccgc ccgaactctc cctgcagcaa
      961 aaaaaatcat catatgacaa cgaaactaag cctccaaaaa gagtataccc tcggcgggga
     1021 actaaaggtc cgccgcatgg gattcggcgc tatgcgcatc accggccagg gcatctgggg
     1081 gccccccaaa gacaaggccg aagccatccg cgtcctccgg cgcgccgtcg aactgggcgt
     1141 caacttcatc gacaacgccg acagctacgg gccccacgta tcggaagacc tgatcgcgga
     1201 agccctctat ccttatccga aaggactggt catcgccaca aagggcggtt tcctgcggac
     1261 gggccccaat caatggcacg tcaattcccg tcccgaacac ctcagggaag ccctcgaaag
     1321 cagcctggcc cgcctcaaac aagaccagat ccatctgtat cagctgcacc gcatcgaccc
     1381 caccatcccc tttcagcgta ccctggaatt cctgcagggc gtccaggaag aaggattggt
     1441 caaatacatc gggctttccg aagtgagtgt ggaggagata aaaaaggcgg aggaatacgt
     1501 cgacatcgtg tcggtacaaa ataaatatag ccaggattac cggaaatggg aatcggtgct
     1561 tcactactgt gaggagaccg acaaggcctt catcccctgg aaccccatca acgcgggcaa
     1621 cctcaccaat atgtccgccg tcgaccgcgt agcgggcaag atcggcgcga cgccccacca
     1681 ggtagccctc gcctggctgc tctatcattc atcaaacaac ttactgatcc ccggcacctc
     1741 gagcgtcaaa cacctcgaag agaacatcgc cgccgaaaat atcgagctca ccgaagagct
     1801 gctggagcaa ctggagaaat aaggccccct accccaacaa tcgctttatc tccccctcca
     1861 tcgcctccgg cttcgtcatc ggcgaatacc gcttcaccgg cttgccggtc ctgtcgacca
     1921 ggaatttcgt aaaattccac ttgatccggc tcccaaaaag gccgcccagt ttcttcttta
     1981 aataacgaaa aacagggtgg gtccccggac cattcacctc gatcttctct actaacggaa
     2041 acgtcacccc gtgattgatc aggcaggtct gctccatcac ctcgttggac aagggttcct
     2101 gcgcgccaaa ctggttacag ggaaatccca gcacgaccag cccctggtcc ttgtactgct
     2161 gatacaattt ctccaaaccc gtgaactgcg gcgtaaaccc gcattgcgtc gccgtattga
     2221 cgataagcac caccttcccc tggtaatcgg ccatgttcag ggcacgcccc tggggagttt
     2281 tagcggacaa ctcgtaaaat tcatttgcca taagatcaat atacaatttt cccggccctc
     2341 cggccggcat cactgccggc aagccggagc cccataaaga accccgggcg tggacccggg
     2401 gttgagatgt taaaagttgg tatgcaatcg ctagactttg caagcagcag gctctctgct
     2461 tatactaaag atactatatt ttagcacatc tccgccgcgg cggtgaaatt tctttcacgg
     2521 tcagtacaac aggcgcccct cttcatcaca accacgagtc ccggtcagca tacctcgcaa
     2581 acttcatcca caaaaatcaa caaaacaaac agtagccaaa aatcgattca atacacactc
     2641 ttatcgaaaa aatcgacacg actatccaac aaacgcattt cttccgcggc ttaatcgccc
     2701 ggattttcca aactgtcccg gtttattcgt atttgttttt tatttaatgt atcgaccgcc
     2761 cgccccgggc ccatcccggt cacacccgga accctcctgt ctccacccgc tccccttcca
     2821 tgccgcaccc gcggcgccac cagcgatccc ggcgccatcg ttcctccacc cggtgcccct
     2881 ttcttcccac cctgcatcac cgtcttcgca tccgccgccg tcttcccacc cggtcccccc
     2941 acgctcgccg tcgaagcctg ctcaaacccg gcccggtaag catacgactt atccatccag
     3001 cggactccca gcaaaaacag gcaggcaaca ggcatcccca ccaacgctac catcgacagc
     3061 ttctttatca gatattgata attccccgcc cggtcatcat ccggaaagat cgaccatcgc
     3121 cactgcctca cgacattctt cggctgcgcc tcgactatag ccgtcatccg ctctacactc
     3181 gccgcctgct cccggcgaaa gacactcatc gcgccatcga ccacctcctt caccggcccc
     3241 acatccgccg gcggcgccgt cacctgcagt tcggttaacc gctgctcgaa agccgccacc
     3301 ctttcttcca accccttcac actctccttc atcaccctaa gccgatcatt cgcctgtttc
     3361 agctcatcca ggatatcatt caataccgtt tcaaaaatta cctcattcat aaaaaagaag
     3421 ttaaaaatga tgccgatggc gatgccgcaa ctgacggtcc tgccgcaaac gctcctcctc
     3481 ccgttgcgcc gactcctggt cttgctgcgc cccctgcttt tctgccgatt cctgccgcgg
     3541 cataccgtcc tgcttttgcg ccaactcctg ctgccgcaac acctcctgct gctccagcag
     3601 ctgtttttgt ctcaactcat tcttccgtaa cgtcgcctcg atctccttcc acggataccc
     3661 ggtctcatat ccccggagaa cgacattgtc ggcatccttg aacgcaatac cccgcacggt
     3721 cttgtaaagc cggtaccctt cctcctccag gcgcttctta aaatcgtcat actgacaaac
     3781 cgactccaat gcctcaccga tcaccttccc cagccggatc acccgttggt cctggctggc
     3841 ttcctgccgc tcctccttcg aacgatacca ccggatcttc ggcgtcggcg tgagccgata
     3901 ctcctgctcc gactccctgc aatagctgtt catccgccgg agcatatatt ggttcgtcac
     3961 cgtatgccgg tcaaacccga tccggt
//
LOCUS       DATGFG010000285         5138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_492819, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000285 DATGFG010000000
VERSION     DATGFG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492819"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VKQ52_05300"
     CDS             complement(<1..276)
                     /locus_tag="VKQ52_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86628.1"
                     /translation="MFSKTYLTVIAVIICFSSCLKKDANSPLMAQSEAIKNAQRYYTD
                     SVVNTLSRGNLVGNPRLTVRRDLLWEHASVLASACGNVVVVPVKYTKP"
     gene            complement(343..1179)
                     /locus_tag="VKQ52_05305"
     CDS             complement(343..1179)
                     /locus_tag="VKQ52_05305"
                     /inference="COORDINATES: protein motif:HMM:NF020914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RteC domain-containing protein"
                     /protein_id="HLZ86629.1"
                     /translation="MNKFFEELSDDLEFRLEHIHSEGEGLASIYQSIEAIKKSLLLMK
                     SFVRDNPFENQSDEVHYYKVVAPPLYGRLFFFMKQYETRMEKQYGSQSKFGDYLQLEL
                     AKIEQFHMRHIEFCFYYIQDAKHMDDRIFVRDAAENTMLDQVEVIMAEDFCVGCYWAA
                     QLLANMEIKRWLERQVEELKGPAVVFSDEDDMPEMVFTDGPTAAVELALALHEKGCFN
                     NGKATLKQVIRWTERYFRIKLGNFHNTIQAIGRRKGETAKFMIELKGEVERKIEELAD
                     RA"
     gene            complement(1276..1881)
                     /locus_tag="VKQ52_05310"
     CDS             complement(1276..1881)
                     /locus_tag="VKQ52_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLZ86630.1"
                     /translation="MEQLIEFLRTIAPLSAALEAHLRLIIALVEYRKGDIILTAGDIC
                     DQIFFIESGLVRSHYYNEDNEEVTNWLMREGDICIAVLSFLRRASSFETHTALEPCRC
                     WSISHEQLYETYRLYPEFIYHGLVITGEYYCRGEERTVYFKMQPKLARYKLFMEHFPE
                     LANRVSNKHLATFLGLRGRSLDDAKKAYLNDLKRDRSRKKN"
     gene            2078..2467
                     /locus_tag="VKQ52_05315"
     CDS             2078..2467
                     /locus_tag="VKQ52_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86631.1"
                     /translation="MAEEQKPRSKQVTMVLSAEDYSRLRKIYEESTCQTFSQFLRETA
                     FHRPIRIKYRNQSIEDFLVVAVDIKNDLEALLRTAGQASADTEIAVISRKVDEIKTVM
                     HQIYQQCTLLSPPPSASGTNSGTTNAK"
     gene            2401..3615
                     /locus_tag="VKQ52_05320"
     CDS             2401..3615
                     /locus_tag="VKQ52_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HLZ86632.1"
                     /translation="MYIAVSTPVGFRHKLRYNERKVEAGKAELIYAGNFILDAERLTL
                     QDKVLRLQRLIELNPRVNHNSLHVSVDFHPRDAPSREQFITIAKEYMEKIGFGRQPYL
                     VYDHRDTTVPHIHILSANIQADGRLIKLYENLMRDPIPITRQLEEKYHLTKARKREKQ
                     TQFNPNAMPPQRLEYGKVETREGLRSALEYLLPRYNYTSLAELNALLRQYNIQAYNGK
                     PGGFMHSHGGLLYQFIDEKGKSMDRGIKASDIAFGPGLRFLEQQFKDNRNKETAAVRD
                     LATRIELTLRRSPAGLSQFIETLRQAGIQTVTYSSHQGQIYGMVFIDHRSKLAVSDQR
                     LGPGLNVDAILVKLGHRQEKERFPDPTLTLETADQRRLLRDMHRSNTLLKDTPAQIEQ
                     QKQQTRDNERSR"
     gene            3627..>5138
                     /locus_tag="VKQ52_05325"
     CDS             3627..>5138
                     /locus_tag="VKQ52_05325"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YWFCY domain-containing protein"
                     /protein_id="HLZ86633.1"
                     /translation="MDNNQDGRSHFDIQGMFRSLAIFLLFLHCYFYCYEAFKSWGYTS
                     AFTDRVLVNISRTGFFYGFHRSRLVALCFLILAMFGVKAKKSQDIKYRQAFLCLGIGL
                     LIYFCVPAVLYSSFIPESSALMYMTGSLLGFILILQGGGLLARILKIGRSNDAFNREN
                     ESFPQEQRRLNSLEFLHFPTTYYYKGRKRKGWINIDPYRSLLVMGSPGSGKTWLIIRS
                     IIRQQIAAGYAMVIFDFKYPDLTQLAYNCYCQYNDTNEQDEKIILPQFYIVSFDDLLR
                     SHRSNPLSPSTMTDIADATELARTLLLALNRDWVEKQGDFWVESAILFLTALIWYLKK
                     WGGGKYCTLPHVIELMQTPYADLFSILRTEPDIASHLSPFLSAYLNDIRKTLDNQISS
                     LKIALSRLSSPALYYIMTGDDFSLEINDPRAPKILCLAGNPQRSGIYGAALSLYITTI
                     NRLVNQKGKTKSSYIYDEAATIRVNQLPEFLATGRSNRISLTLCVQDASQLKLA"
BASE COUNT         1333 a         1480 c         1092 g         1233 t
ORIGIN      
        1 tggcttcgta tattttactg gaactacaac aacatttcca caagcgcttg ccaggacact
       61 cgcgtgttcc caaagaagat cccttcggac ggttagtcgg ggatttccta caaggttccc
      121 tcgggaaaga gtattgacaa cactatcagt atagtatctt tgggcgtttt taatagcttc
      181 gctctgggcc attaacggcg aattcgcatc tttttttaaa caagaggaaa agcagataat
      241 aactgcgatg actgttaaat aagtcttgga gaacatagat taaggtttat agataaacaa
      301 ataaagacca aatcacatac gccattacca tggtttaata cttcaggccc gatccgccaa
      361 ttcctcgatc ttcctctcca cctccccctt caactcaatc ataaacttcg ccgtttcccc
      421 cttccgcctt ccaatcgcct gtatcgtatt atgaaaattc ccaagcttga tcctaaaata
      481 ccgctccgtc caccttatca cctgcttcaa cgtcgccttc ccgttattaa aacacccttt
      541 ctcatgcaat gccaacgcca actccaccgc agcggtcggt ccgtccgtaa acaccatttc
      601 cggcatatcg tcctcgtcac taaacaccac cgccggcccc ttcaattcct ccacctgccg
      661 ctccaaccac ctctttatct ccatattggc tagcaattgc gccgcccagt aacagccgac
      721 acagaaatct tcggccatga tcacctccac ctggtccagc atcgtatttt ccgcagcatc
      781 ccggacaaaa atgcggtcat ccatatgctt tgcatcctgg atatagtaaa agcagaactc
      841 gatgtgccgc atatggaatt gctcaatttt cgccagttcc agctgcaggt agtccccgaa
      901 cttgctttgg ctgccatatt gcttctccat tcgcgtctca tactgcttca tgaaaaagaa
      961 cagcctgccg tatagcggcg gcgccaccac cttgtaataa tgcacttcat ctgactggtt
     1021 ctcaaacggg ttatcccgta caaaggactt catcagcaaa agactttttt taatagcctc
     1081 gatggactga tagatgctgg ccaatccctc tccctcggag tggatatgtt ccaggcggaa
     1141 ttcaagatcg tcggaaagct cttcaaaaaa tttgttcata acggataagt ttggtcgata
     1201 caaatctaaa cagactcata catccgacca ataggggaga gcagttcacg caaaaatgcg
     1261 tactcaccgt tatgactagt tctttttcct ggaccggtcc cgttttaggt cattcagata
     1321 cgcctttttc gcgtcatcga gcgacctgcc tcttagccca aggaaggtgg caagatgttt
     1381 attcgatacc cggttggcta actctggaaa atgctccatg aaaagtttat accgggctag
     1441 ctttggctgc atcttgaaat aaacagtccg ctcctcgccc cggcagtaat attcgccggt
     1501 gatcaccaat ccgtggtaaa tgaattccgg gtatagccgg taggtttcat acaattgttc
     1561 atggctgata ctccaacacc ggcatggctc gagggccgta tgcgtctcaa acgaagacgc
     1621 ccggcggagg aaactcagca ctgctataca gatatccccc tcccgcataa gccaattggt
     1681 aacctcctca ttatcttcat tataataatg acttcgcaca agacccgatt cgataaaaaa
     1741 gatttgatcg cagatatcgc ccgcggtaag gataatatcg cctttacgat attccaccag
     1801 cgcaattatc agtcggagat gcgcttccag ggcagcggat aagggcgcga tggtacggag
     1861 aaattcgatg agttgttcca tgtctataat ttattgtggt tatgtcgaaa atttattatg
     1921 aatatgtcaa acgtcatagc aaaacttttc caccaataaa ggaatctcct taaaaactgc
     1981 agtattcttt cagtcggcga ctgtagatga ccgttattta accatttcag ctggataata
     2041 ccgcggcgct ttacattagc atgtaaacag acacaagatg gccgaggaac aaaaaccacg
     2101 gagcaaacag gtgaccatgg tcttgtccgc agaggactat tcccgcctgc ggaaaatata
     2161 tgaagagagt acttgccaga ccttttccca gtttctgcgg gaaaccgcct ttcacaggcc
     2221 gataaggata aagtaccgga atcagtccat cgaggatttt cttgtcgttg ccgtcgatat
     2281 aaaaaacgac ctggaagctc ttttgcggac agcaggccag gcaagcgcgg atacggaaat
     2341 tgccgtcatc agccggaaag tagatgaaat aaagaccgtc atgcaccaaa tctatcaaca
     2401 atgtacattg ctgtctccac ccccgtcggc ttccggcaca aactccggta caacgaacgc
     2461 aaagtagaag ccgggaaagc cgaacttatc tacgccggca atttcatcct cgatgcggag
     2521 cggcttaccc ttcaggataa agtgcttcgc ctgcagcggc tcatcgaact caatccgcgg
     2581 gtgaatcaca attccctgca tgtttcggtc gattttcatc cccgtgacgc tccctcaagg
     2641 gaacagttca tcactatagc aaaagagtac atggaaaaga tcgggtttgg gcgtcagcct
     2701 tacctcgtat atgaccatcg ggatacaacg gtccctcata ttcatatcct gtcggccaac
     2761 attcaagccg acggccgcct gatcaagtta tatgagaatt tgatgcgcga cccgatcccc
     2821 atcacccggc aactggagga gaaatatcac ctcacaaagg cccggaagcg ggaaaagcaa
     2881 acccaattta acccaaacgc catgcccccg cagcgcctgg aatatggtaa agtcgagacg
     2941 cgagagggcc tgaggtcggc attggaatat cttcttccga ggtataatta cacatccctt
     3001 gcggaattga acgcccttct ccggcaatac aatatccagg cgtataatgg caagccgggg
     3061 ggatttatgc atagccacgg tggcctgctt tatcagttta tagacgaaaa aggaaagtcg
     3121 atggatcggg gcataaaagc cagcgatatc gccttcggcc ctggactcag atttctcgaa
     3181 caacaattca aggacaatcg gaataaagag acagccgccg ttcgcgacct tgccacccgc
     3241 atcgaactga cccttcggag aagcccggcc ggcctgtcgc aattcatcga aaccctccgg
     3301 caggccggaa tccaaaccgt cacctattcc agccaccagg gacagattta tggaatggtc
     3361 ttcatcgacc atcgttcaaa acttgccgtt tccgatcaac gtctcggccc gggcctgaac
     3421 gtcgatgcca tcctggtaaa acttggccac cggcaggaaa aggaacggtt ccccgatcca
     3481 acgctcacgc tcgaaaccgc cgaccagcgc cggctgctcc gtgacatgca tcgttcgaat
     3541 accctgctta aggatacacc ggcgcaaata gagcaacaaa aacaacaaac ccgggacaac
     3601 gaacgatccc gataacaaaa gagtatatgg acaacaatca ggacggccgc agtcacttcg
     3661 acatccaggg tatgttccgc tccctcgcca tttttcttct ttttctccac tgttacttct
     3721 actgctatga ggcattcaaa agctggggct atacctctgc tttcaccgat cgggtccttg
     3781 tcaatatcag ccgaacgggc tttttttacg gctttcatcg ttcccggctc gtcgccctct
     3841 gttttcttat tttagccatg ttcggcgtca aggcaaagaa atcacaggac atcaaatatc
     3901 gtcaagcctt tctctgtctg ggcattggcc tgctgatcta cttctgcgta ccggccgtcc
     3961 tttattcctc attcatcccc gaatcctctg ccctcatgta tatgacgggc agcctcttgg
     4021 gttttattct catcctgcag ggtgggggac tccttgctcg catcctgaag attggtcgct
     4081 ccaatgatgc tttcaaccgg gagaacgaat ccttccccca ggaacagcgt cggcttaata
     4141 gcctggagtt cctgcatttc ccgactacgt attactataa ggggcgaaag cgcaaaggct
     4201 ggatcaatat cgacccatat cgtagcctgc tggtaatggg ctcccccggc agtggtaaaa
     4261 cctggctcat catccgcagc attatccggc aacagatcgc cgccggctac gcgatggtca
     4321 tcttcgactt caaatacccc gatcttaccc agttggcata caactgttac tgccaataca
     4381 atgacacaaa cgaacaggac gaaaaaataa ttctccccca gttctacatc gtcagctttg
     4441 acgatctcct ccggtcgcac cggtccaacc cgctgtcgcc ctctaccatg accgacatcg
     4501 cagatgctac cgaactggcc cggacactcc tcctggccct caaccgcgac tgggtcgaaa
     4561 aacaaggcga cttctgggtc gaatcggcca ttctatttct gacagccctc atctggtatc
     4621 tgaagaaatg gggaggcggc aaatactgta ccctgccgca cgtcatcgaa ctcatgcaaa
     4681 ctccttacgc cgacctcttt tcgatcctcc gcaccgagcc ggatatagcc agtcatctca
     4741 gccccttcct ctcggcttac ctcaacgaca taaggaaaac cctcgacaac cagatctcct
     4801 ccctgaagat cgctctctcc cgtctcagtt ctccggcact gtattatatt atgaccggcg
     4861 acgacttctc tttggagatc aatgatccca gggcgcccaa aatactctgt ctcgccggca
     4921 atccccagcg ttccgggata tatggcgcgg ccttgtcgct gtatattaca actatcaacc
     4981 ggctcgtaaa ccagaaggga aagacaaaaa gcagctatat atatgacgaa gcggccacca
     5041 tccgggtcaa tcaacttcct gaattcctcg ccacaggccg ctcgaaccgc atatccctca
     5101 ccctctgcgt ccaggatgcc agccagctca agctcgcc
//
LOCUS       DATGFG010000286         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1522223, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000286 DATGFG010000000
VERSION     DATGFG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522223"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4129
                     /locus_tag="VKQ52_05330"
     CDS             <1..>4129
                     /locus_tag="VKQ52_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HLZ86634.1"
                     /translation="LAAQHYNYDPNEPSTNNNVRVSDGEGDEEGGSDYSASDDFTFGD
                     VVYQQEVDTRSLPKSSSSGGRLLNLNIVDKLPEFKGIYHVLIRSAKNYWVSDKRFVSL
                     SDIGLIAKEGREKILVFANSIKNASALPGVNVLAYGNNNQLLGMGTTSAEGVAEITYT
                     RKELAGFKPAMIIAKTPDDFNYLPFSTTHVNTSRFEVGGRRSNPSGLDAFVYPERDIY
                     RPGEKVHFAVILRDHQWRSPGELPIQLKFLLPNGKEMKTFRKTLNSQGTADGEIELPE
                     SAITGSYSLEVYTSNDVLLTSQPFRIEEFVPDRIKVTAKLDKPFLEAGDVSRLDIHAV
                     NFFGPPAANRNYECEIQVKQQDFRSKNYRNYDFSLSAQNSTSFDKTVREGKTDEQGNA
                     VEKYTVPEFFRNIGLLQATFYATVFDETGRPVSRSSSVNIYTQPVFFGVADDGYYYYP
                     LNQSVQFPIIALDRTEKLLNGVTARVQVIKHEYRTVLTKSGNYFRYESQKEDKQITSG
                     IVTVSGESSRYSFVPRSPGDYEIRVSIPGANTYVSRSFYSFGAWGGDNNSFDVDNEGH
                     VDIQLDKAGYYTGESVKALFKTPFSGRMLVTMETDKVVSYKYVDVDKRTASLDLPLTV
                     DDIPNVYITATLIKPHELSDIPLTVAHGYQSVKVEAKDRKIPVDIVAQEKVRSHTHQK
                     VRVKAAPGSMVTLAAVDNGVLQVTDFGTPDPYAHFYAKRALEVNGYDLYPLLFPEVKP
                     TLSSTGGDGESDMKKRVNPMPAKRIKIVSYWSGIAQADGSGEAEFEFDIPQFSGQVRL
                     MAVACKDDRFGSKESAITVADPVVLSSALPRFMSPGDTVTMPVTITNTTNSSASATAK
                     LRLGGDLRTIGEQQQTISIGAGSEGRATFQLLAPPAIGTGKVTVDVSSMGEHFTDETE
                     IGIRPSAPLQKTTGAGSVGGGGTQSIPFTTGDYIPSTIDYRLVVSRSPAAELGKYLSL
                     LVEYPYGCTEQTVSVAFPQLYFGDLADLVQKSDVNGYQRKSANANILEAIRKIKMRQL
                     YNGAVTLWDNEDTENWWTTVYAAHFLLEAQKAGFDIDKGLISTMLGYINSRMKNRETV
                     TYYYNRDQTKRIVPKEMVYGLYVLSLAGQPNVSVMNYYKAHASQLSLDCQYLLSAAYA
                     LAGDKKSFKEFLPTQFTGEESVAQTGGSFYSDIRDESLALDVLVEADPENAQIPVMAR
                     HVMEKLKTRYWLSTQELAFSFLGLGKMAHQSAGSTIAGDIRVDGRVVASMTGNPVKLT
                     TKDLRGSGGGLPHVDIATRGNGKLYYYWEAEGISASGAYKEEDNYLRVRKRFYDRYGR
                     PITGNTFKQNDLVIVQLTLERTFSTDVDNIVITDMLPAGFEIENPRTKELPGMDWI"
BASE COUNT          968 a         1352 c         1084 g          725 t
ORIGIN      
        1 ttggccgccc agcattataa ctatgacccg aacgagccct ccaccaataa caatgtccgc
       61 gtcagcgacg gggaaggcga tgaagaaggg gggagcgatt acagcgccag cgacgacttc
      121 acctttggcg atgtcgtcta ccagcaggag gtcgataccc gcagcctgcc caaaagcagt
      181 agcagcggcg gccgcctgct caacctgaac atcgtggaca agctccccga gttcaagggc
      241 atctaccacg tgttgatccg cagcgcaaag aactattggg tcagtgacaa acgctttgtt
      301 tccctctccg acatcggtct catcgcaaaa gaaggccggg aaaagatcct cgtcttcgcc
      361 aactccataa agaatgcatc ggccctgccc ggcgtcaatg tcctcgccta cgggaacaac
      421 aaccagctgc tcggtatggg taccaccagc gccgaagggg tagccgagat cacctatacc
      481 cggaaggaac tggccggctt caaaccggcg atgatcatcg caaaaacccc cgatgacttc
      541 aactacctgc ccttcagcac tacccacgtc aacacctccc ggttcgaggt gggcggccgc
      601 cgcagcaatc cctccggcct cgatgccttt gtctatcccg aaagggatat ctatcgcccg
      661 ggggagaagg tacacttcgc cgtcatcctt cgtgaccacc agtggagatc tcccggggaa
      721 ctgcccatcc agctgaaatt cctgctgccc aatggtaagg agatgaaaac cttccgcaag
      781 accctgaaca gccagggcac ggcggatggg gaaatagagc tccccgaatc ggccatcacg
      841 ggcagctact cgctggaggt ctatacctcc aatgacgtcc tcctcaccag ccagcccttc
      901 cgcatcgagg aattcgtacc ggacaggatc aaggtcacgg ccaaactcga caaacccttt
      961 ctcgaagccg gcgacgtctc ccgcctggat atccatgccg tgaatttctt cgggccgccc
     1021 gccgccaacc gcaattacga atgcgagatc caggtaaaac aacaggattt cagatcgaag
     1081 aactatcgta attacgattt cagcctctcc gcccagaact ccacctcttt cgacaagacc
     1141 gtgcgcgaag gcaagaccga cgaacagggg aatgccgtcg agaaatatac tgttccggaa
     1201 ttcttccgga atatcggcct gctccaggct accttctatg ccaccgtctt cgacgaaacc
     1261 ggccgccccg tcagccgcag cagctcggtg aacatctata cccaacccgt cttcttcggc
     1321 gtggccgacg atgggtacta ttactatccc ctgaaccagt ccgtacagtt cccgatcatc
     1381 gccctcgacc gcaccgaaaa actgctgaac ggcgtcacgg cccgggtgca ggtgatcaag
     1441 cacgaatacc gcaccgttct caccaagagc ggcaactatt tccggtatga atcgcagaag
     1501 gaggataaac agatcaccag tggcatcgtt acggtcagcg gcgagagcag ccgctacagc
     1561 ttcgtccccc gcagccccgg cgactatgaa attcgcgtct ccatccccgg tgcaaacacg
     1621 tatgtcagcc gcagcttcta cagctttggc gcctggggcg gcgacaacaa ttccttcgac
     1681 gtcgacaacg aggggcatgt cgatatccaa ctggacaagg cgggctacta caccggcgaa
     1741 agtgtaaaag ccctgttcaa gacgccgttc agcggacgca tgctcgtcac catggagacg
     1801 gacaaggtcg tctcctataa atatgtcgat gtcgacaaac gcaccgcctc cctcgatctg
     1861 ccgctcacgg tggacgatat ccccaacgtc tatatcaccg ccacgctgat aaaaccacac
     1921 gagctctcgg atatccccct aacggtcgcc cacggctacc agtcggtaaa ggtcgaggca
     1981 aaggacagga agatacccgt cgatatcgtc gcgcaggaga aggtccgcag tcatacccac
     2041 cagaaggtgc gggtaaaggc cgcacccggc agcatggtca ccctcgcggc cgtggacaat
     2101 ggtgtcctgc aggtgaccga cttcgggacc cccgacccct atgcccattt ctatgcaaaa
     2161 cgggccctgg aagtaaacgg ctacgacctc tatcccctgc tcttccccga agtgaaaccc
     2221 accctcagca gcacgggcgg tgacggcgaa agcgatatga agaaacgcgt caaccccatg
     2281 cccgccaaac ggataaagat cgtctcgtac tggagcggca tcgcccaggc cgatggcagc
     2341 ggcgaggccg aattcgaatt cgacatcccg cagttctccg ggcaggtacg cctcatggcg
     2401 gtggcctgca aggacgatcg cttcgggtca aaagaatccg ccatcacggt tgccgacccc
     2461 gtcgtgctta gctcggccct gccccgcttt atgagtcccg gcgatacggt gaccatgccc
     2521 gtcacgatca ccaacacgac aaacagctcg gccagcgcca ccgcaaaact caggttgggc
     2581 ggcgacctcc ggaccatcgg cgaacaacag cagacgatca gcatcggcgc cggcagcgaa
     2641 ggccgggcca ccttccagct cctcgcacct ccggccatcg gtacaggcaa ggtcacggtg
     2701 gatgtctcca gcatgggcga acacttcacc gatgagaccg agatcggtat ccggccctcg
     2761 gcgcccctgc aaaagaccac cggcgccggc tctgtcggcg gcggcggcac ccagtccatt
     2821 cccttcacga ccggcgacta tatcccttcg acgatcgact accggctggt ggtcagccgt
     2881 tctccggcgg ccgaactggg caaatacctc agcctgctcg tcgaataccc ctatggctgt
     2941 accgaacaga ccgtatccgt cgccttcccg cagttgtatt ttggcgacct ggccgacctt
     3001 gtccagaagt cggatgtcaa cggctaccag cgcaaatcag ccaacgcgaa tatcctcgag
     3061 gcgatccgga agatcaagat gcggcaattg tataacggcg ccgttacgct ctgggacaac
     3121 gaggacacgg aaaactggtg gaccaccgtc tatgccgcgc acttcctact cgaagcccag
     3181 aaagccggct tcgatatcga caagggtctc atcagcacca tgctcggcta tatcaacagc
     3241 cgcatgaaga accgcgagac cgtcacgtat tattacaacc gcgaccagac aaaacgcatc
     3301 gttccgaagg aaatggtcta tggtctctac gtcctctcgc tggccggcca gcccaatgtc
     3361 agcgtcatga actactataa ggcacatgcc tcgcaactca gtctcgactg tcaatacctg
     3421 ctctcggcgg cctacgccct ggcgggggac aagaaaagct tcaaggaatt cctgcccacg
     3481 cagttcaccg gcgaggaatc cgttgcccag acgggcggca gcttctattc ggacatccgc
     3541 gacgaatccc tggcgctcga tgtgctggtc gaggccgatc ctgaaaacgc ccagatcccc
     3601 gtcatggccc gccatgtcat ggaaaagctg aagacacgct actggctgag cacccaggaa
     3661 ctggccttta gcttcctcgg cctgggtaag atggcccacc agtcggcggg ctccaccatc
     3721 gcgggcgata tccgcgtcga cggccgggtc gtcgcctcga tgacgggcaa ccccgtcaag
     3781 ctgacgacaa aggacctccg cggttcgggc ggcggcctgc cccatgtcga catcgccacc
     3841 cgcggcaacg gcaaactcta ttattactgg gaggccgaag gcatcagcgc cagcggcgcc
     3901 tataaggagg aggacaatta tttgagggtg cggaaacgtt tctacgatcg ttatggtcgt
     3961 ccgatcaccg gcaacacctt caagcaaaac gacctggtca tcgtgcagct aaccctcgaa
     4021 cgcaccttca gcaccgatgt cgacaatatc gtcatcaccg acatgctgcc cgcgggcttc
     4081 gagatcgaga atccccgcac aaaagaactg ccgggtatgg actggataa
//
LOCUS       DATGFG010000287         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1631875, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000287 DATGFG010000000
VERSION     DATGFG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631875"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1144)
                     /locus_tag="VKQ52_05335"
     CDS             complement(158..1144)
                     /locus_tag="VKQ52_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HLZ86635.1"
                     /translation="MHYSDYLQLDKILDAQHPESDRLGMHAHDEMLFIVIHQTYELWF
                     RQLLYETESVIGIFGQPALNDNSPELQTIVHRLSRCATILKVLVHQIDIMETMTPMDF
                     QDFRDLLRPASGFQSVQFKLLEARLGLRYEHRYGQGYYLSQLRPAEAERIRAAEQSPS
                     LLPLVNAWLERMPFLGEMDSDRFWSDYRHRYETSLAEGEKGNLNGFDSVFVGSDTVRS
                     LSAAACRAALFIMLYRGYPLLQLPFQVLHQLLEIDEQLSTWRYRHMSMVQRTIGARIG
                     TGGSTGKDYLSAALERHHLFREIAALTSFLLQRKRLPELAPEMQRRLGFAGA"
     gene            complement(1153..>2238)
                     /gene="purD"
                     /locus_tag="VKQ52_05340"
     CDS             complement(1153..>2238)
                     /gene="purD"
                     /locus_tag="VKQ52_05340"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HLZ86636.1"
                     /translation="VGPEEPLVKGIADFFLADAALRKIPVIGPTAKGAQLEGSKAFSK
                     KFMVRHGIPTAAYQEFSTDNFAAGIAYLQQHPLPVVLKADGLAAGKGVVICQSTIEAV
                     AEFELMLQGNKFGEAGNKVVVEEFLTGIELSVFVLTDGKHFLILPEAKDYKRVGEEDT
                     GPNTGGMGAVSPVPFADAEFMEKVTGRIIVPTVKGLAEEGIEYKGFIFIGVIKVGDDP
                     FVIEYNCRMGDPETEVVMPRLKNDLVELFLAVGRQGLNELTIGQEERTACTIVAVSGG
                     YPGSYQKGLPISGLDQLPAGNGDSIVFHAGTRAEKDTVLTNGGRVLCVTSFAETVYEA
                     VDKSRDVLEKIYYDGIYYRRDIGYEFL"
BASE COUNT          488 a          682 c          618 g          450 t
ORIGIN      
        1 gataaaacaa tcgtcatcgt atctgcaagg ggtataaaaa actacctata ggatcgaagg
       61 gcccggaaag cctcatacaa gggccgtcac agacgaggcg cctacgcggt acaagcaccg
      121 tcacagacgc ggcacctacg aggcgcctac gaggaaccta cgctcccgcg aaacccaacc
      181 gccgctgcat ttcaggagca agttccggta gccttttccg ttgaagcagg aagctggtaa
      241 gggcggcgat ctcccggaag aggtgatggc gttcgagagc ggcggagaga taatccttgc
      301 cggtgctgcc acccgtgccg atgcgggcgc cgatcgtccg ctggaccata ctcatgtgac
      361 gataacgcca ggtgctgagt tgttcgtcta tctccaacag ttgatgcagc acctggaacg
      421 gcagttgcag caagggatag ccgcgataga gcatgataaa aagcgcggcg cggcaggcgg
      481 cggcggaaag gctccggacg gtatccgaac caacaaacac cgagtcgaag ccgttcaggt
      541 tgcccttctc tccttcggcc aggcttgtct cgtaacgatg gcgatagtcg gaccagaacc
      601 ggtcggaatc catctcaccc aggaagggca tccgctccag ccaggcgttc accaggggca
      661 gcagggatgg cgactgttcg gcggcacgga tcctctccgc ttccgccggc cgcaattggg
      721 aaagatagta cccctgtccg taacggtgtt cgtaacgaag gcccagccgg gcttccagca
      781 gcttgaactg aacgctctga aagccggaag caggcctcag cagatcgcgg aagtcctgga
      841 aatccatggg agtcatcgtt tccatgatat cgatctggtg caccagcact ttgaggatgg
      901 tggcgcagcg cgaaagccgg tggacgatgg tctgcaattc cggggagtta tcgtttagcg
      961 ccggctgccc gaaaatgccg atcaccgact ccgtctcata caacagctga cggaaccata
     1021 actcataggt ctggtggatg acgataaaga gcatttcgtc atgggcatgc atacccagcc
     1081 ggtcgctctc gggatgctgg gcgtcgagta tcttgtcgag ctgcaggtaa tcgctatagt
     1141 gcatcgttgg ttctataaaa attcataccc gatatcccgg cgatagtaga tcccgtcata
     1201 atagatcttt tccagcacat ccctggactt gtccaccgcc tcgtagaccg tttcggcaaa
     1261 agaagtgaca cataacaccc ggccgccatt ggtaagaacc gtatcttttt cggcgcgggt
     1321 gccggcatga aagacaatac tgtcgccgtt ccccgccggc agttggtcga gcccgctgat
     1381 cggcagccct ttttgatagg aaccgggata ccccccgctc accgcaacga tcgtacaggc
     1441 cgtacgctcc tcctggccga tggtcagctc attaagaccc tgcctgccga cggccaggaa
     1501 caattccacc aggtcgttct tcagccgggg catcacgacc tcggtttccg ggtcacccat
     1561 ccggcaattg tattcgatca caaagggatc atcgccgacc ttgatgaccc cgataaaaat
     1621 aaatcctttg tactcgatgc cctcttccgc cagacccttc accgtcggaa cgatgatcct
     1681 gccggttact ttctccatga attccgcatc cgcaaagggc accgggctga cggcgcccat
     1741 cccaccggta ttgggaccgg tatcctcttc ccccacccgt ttatagtcct ttgcttcggg
     1801 caggataaga aaatgtttcc cgtccgtcag aacaaagacg gacagctcga tccccgtaag
     1861 gaattcctcc accacgacct tgttcccggc ctcgccgaac ttgttgccct gtagcatgag
     1921 ttcgaattcc gccacggctt cgatggtgct ctggcagatg accaccccct ttccggcagc
     1981 cagcccatcg gccttcagta ccaccggaag gggatgctgc tgcaaatagg cgatccccgc
     2041 ggcaaagttg tcggtgctga actcctggta ggccgcggtc gggatgccgt gtcgcaccat
     2101 gaatttcttg gaaaaggcct tgctgccctc cagctgggct cctttggcag tgggcccgat
     2161 caccgggatc ttccgcagcg cggcgtcggc aaggaagaag tcggcgatcc ccttcaccaa
     2221 cggctcctcg ggtccgac
//
LOCUS       DATGFG010000288         2783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_44844, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000288 DATGFG010000000
VERSION     DATGFG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44844"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VKQ52_05345"
     CDS             complement(<1..1041)
                     /locus_tag="VKQ52_05345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLZ86637.1"
                     /translation="MKVVQVLPFFLPDYVGGTEVYCWSLCKYLQSRGVDVEVLIPHYR
                     STETTRYVYDGIPVTKYAEPTELSKAVIAGLVPPEGLKVYKALLAQLKPDIVHFHGIY
                     GSVGITVPHIAAARELGCRTLYTMHLANHVCRTQTLVYKGRDLCDGVIRPYRCASCAL
                     QFGGVNGVVGRLLPAVSYLLAGAGADTAKWNTRLGTGLSAVNRIRDLQRDLITIAEEC
                     DKVVVIAKWFEAIMRKNGFPADRLAFIAPALTYSADRPGEEEPVRFHRPDTLRLIFIG
                     RLFPTKGLDLLLRAMAGLPEEKIELSIYGKDDDADYFRQCQALAAGKKNIHWRGVAPR
                     EKVMAIFSQHDAL"
     gene            complement(1089..1880)
                     /locus_tag="VKQ52_05350"
     CDS             complement(1089..1880)
                     /locus_tag="VKQ52_05350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ86638.1"
                     /translation="MANLSIFFRYSVVNFFLFLKNLLLYPFSRLARHRLSHSYQVITY
                     PFFGDRYVELSELLKDENMQVSLAPLKARPHNTTEFELVSIAAIIKDNRCNTILEIGT
                     FDGRTTRAMAMNLADENGKIYTLNLPPEMDEVSLSTHQIDVNLASKVVSGERFLGTPQ
                     ERYIQQIWGDSAKFDFSPYNGKMDLVFIDGAHSEDYVANDTKKAFQLVKPAGGIILWH
                     DAHLFGVKDFFTKWLAETHYPVYFIRNTSVAVMGLKNGQPFDLKA"
     gene            complement(1898..>2783)
                     /locus_tag="VKQ52_05355"
     CDS             complement(1898..>2783)
                     /locus_tag="VKQ52_05355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ86639.1"
                     /translation="TSYNREKYIAEAIESVLRSSYTNYELIICDDRSTDRTVEIARSY
                     AARDPRIKVYVNEKNLGDYPNRNRVASYARGKYLKYQDSDDIIYPHTLQIMVDHMENF
                     PEVSVAFCGNEIQDYSQPYPVCFSPQEAYRTHFFDRNSLFYQGPVATIIRRETFERYG
                     PFSGVRYVGDAEMWMIFARYTPVLKIQPGLIWWRTHPGQEFSMGTEDYVLLKYQLNKQ
                     VLEHPDCPLTEVERNAALGNNRRLLGRKILRLVFGRLAISSAIKLYRDAGLKPAILLE
                     SVIPVNRLKRLNGKLARP"
BASE COUNT          597 a          793 c          782 g          611 t
ORIGIN      
        1 gagggcgtca tgttggctga aaatagccat caccttttcc ctcggcgcaa ccccgcgcca
       61 atggatattc tttttaccgg ccgccagtgc ctggcattgc cggaaataat ccgcatcgtc
      121 gtccttgccg taaatactca attcgatctt ttcttccggc agcccggcca tggcgcggag
      181 caggagatcc aacccctttg tcggaaagag cctgccgata aagatcagcc gcagggtatc
      241 cggccggtga aagcgaaccg gctcctcctc gcccggcctg tcggccgagt aggtcagcgc
      301 gggagcgatg aacgcgaggc ggtcggcggg aaaaccattc tttcgcatga tggcctcaaa
      361 ccattttgcg atcaccacca ccttgtcgca ttcttccgcg atggtgatca gatcccgctg
      421 cagatcgcgt atgcggttca ccgccgataa ccccgtcccc aggcgggtat tccatttcgc
      481 cgtgtcggcg ccggcgcccg caagaagata gctcacggcg ggaagaagac ggccgacgac
      541 gccgttgacc ccgccgaatt gcagggcgca ggaggcgcag cgataaggcc ggatcacgcc
      601 gtcgcacaga tcccggcctt tatagacaag ggtctgggtc cggcatacat gattggccag
      661 gtgcatggtg taaagggtcc tgcaacccag ctcccgggcc gccgcgatat gcggaacggt
      721 gatgccgacg cttccataga tgccgtgaaa atggacgatg tccggcttca gctgcgcaag
      781 cagcgcttta tagaccttca gcccttcggg tgggacaagc cccgcgatga cggccttcga
      841 tagttccgtc ggctcggcat atttggtgac gggtatccca tcatacacat aacgcgtcgt
      901 ctccgtgctc cggtaatgcg ggatcagcac ctcgacatcg acgccccggg attggaggta
      961 tttgcacagg gaccagcaat acacttccgt accgccgaca taatccggta aaaaaaaggg
     1021 aaggacctgg acgactttca tcagcagatt cagttatgca tttcgtgagc aacccggact
     1081 ctgtccgatt atgccttgag atcgaaaggc tgtccgtttt tcagccccat cacggccacc
     1141 gacgtattcc ggatgaaata aaccgggtaa tgggtctcgg ccagccactt ggtgaaaaaa
     1201 tcctttacgc cgaacaaatg cgcatcgtgc cagagaatga tgccgcccgc cggcttcacc
     1261 agctgaaagg cctttttcgt gtcgttggcc acatagtctt cgctatgggc cccatcgata
     1321 aagacaagat ccatcttgcc gttgtaaggg gaaaagtcga atttggcgga gtcgccccat
     1381 atctgctgaa tgtatcgctc ctgcggtgtg ccgaggaatc gctccccgct gacgaccttg
     1441 gaggccaggt tgacatctat ctggtgggtg gaaagactca cttcatccat ctccggcggc
     1501 aggttcagcg tgtagatctt cccgttctca tcggccaggt tcatcgccat ggccctcgtc
     1561 gtcctgccgt caaaggtccc gatctccagg atcgtattgc aacggttgtc cttgatgatc
     1621 gcggcaatgc tcaccagctc gaattccgtc gtattatgcg gtcttgcctt caacggggca
     1681 agactcacct gcatgttctc gtctttcagc agctcggaaa gctcgacata gcggtctccg
     1741 aaaaagggat aggtgatcac ctggtaggaa tgggaaagcc ggtgacgggc cagccgggaa
     1801 aagggataga gcaacaggtt cttcaggaag aggaagaaat tcactacgga gtatctaaaa
     1861 aaaatagaaa gattggccat tgggttatat tagtatgtta tggtcttgcg agtttcccgt
     1921 tcaggcgctt cagccggttg acggggatca cggattccag caggatagcg ggcttcagcc
     1981 ccgcatcccg gtatagtttt atcgcggagg agatcgccag cctgccgaac acaagccgca
     2041 ggatcttcct gcccagcagc cgccggttat tgcccagcgc cgcatttctt tccacctcgg
     2101 tgagcgggca atcgggatgt tccaacactt gtttgttgag ctggtatttg agcagtacat
     2161 aatcctccgt tcccatcgag aactcctggc ccggatgcgt ccgccaccag ataagccccg
     2221 gctggatctt caggacgggg gtatatcggg caaagatcat ccacatctcc gcatcgccga
     2281 cataccgcac gccggagaaa gggccatagc gttcgaaggt ctcccgccgg atgatggtgg
     2341 cgacggggcc ctggtagaaa aggctgttcc tgtcgaagaa atgggtccgg tacgcttcct
     2401 gtggactaaa gcagacgggg tagggctggg aataatcctg gatctcgttt ccgcagaaag
     2461 cgaccgatac ctccgggaaa ttctccatat ggtccaccat gatctgcagc gtatgaggat
     2521 aaatgatatc gtccgagtcc tggtatttca ggtatttccc cctggcatag gaggccaccc
     2581 ggttgcggtt gggataatct ccgaggttct tctcattcac atataccttg atccgcgggt
     2641 cccttgcggc atagctgcgg gcgatctcca ccgtcctgtc cgtcgaccgg tcgtcgcaga
     2701 tgatcagctc atagttcgta taggaagagc gcaggacgct ttcgatcgcc tcggcgatat
     2761 acttctcccg gttgtaggag gtc
//
LOCUS       DATGFG010000289         7741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1179764, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000289 DATGFG010000000
VERSION     DATGFG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179764"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VKQ52_05360"
     CDS             complement(<1..255)
                     /locus_tag="VKQ52_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86640.1"
                     /translation="MLLFCPHNTPRLRYIVDFCAKELFNAPIRITTDWDEFTAAGDAR
                     INYSGHPPINEAVNITPTGLLFATGIDPQPITCFEYKDRKA"
     gene            complement(278..1657)
                     /locus_tag="VKQ52_05365"
     CDS             complement(278..1657)
                     /locus_tag="VKQ52_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HLZ86641.1"
                     /translation="MTETAANFIARSTIKAEDLEHRKKINFNIGRYNSVVPQGKQQFA
                     ELNLARERGKNIKWRALETLDQQLEEFESHFTRRGGRVIWAENTEQALAEILNICREK
                     NCRTIVKSKSMVTEEIKLNHFLEANGIESIETDLGEYIQQLDGEPPYHIVTPAMHKSK
                     EDVAKLFYEKLNTRPGLTPEQLTLVAREKLRKKYVQAEIGITGANFLISDIGGVAVTE
                     NEGNARLSASFPKTHIVIAGLEKVIPSMTDLGLFWPLLATYGTGQRVTVYNTIITGPR
                     QPGEPDGPEEMILILLDNGRTNILANAKARESLYCIRCGACLNACPIYKNIGGHSYGT
                     TYSGPIGSVITPHLKDMGEWKHLSYASSLCGNCTEVCAVKINLHELLLDNRYEAVKDG
                     YATWSEKLAWKVWKAASLSRTRMNMGNPRIKNWMVNKAFKAWTAHRDQLEFSPQTFNQ
                     RWKAERAKG"
     gene            complement(1654..2259)
                     /locus_tag="VKQ52_05370"
     CDS             complement(1654..2259)
                     /locus_tag="VKQ52_05370"
                     /inference="COORDINATES: protein motif:HMM:NF022751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HLZ86642.1"
                     /translation="MIAIDNILVSDDVVREQFVCDLLKCKGGCCEDGDAGAPLEKAEL
                     DEINAAYEIVKPYLTPAGIKEIETRGRYQYDREFGWVTPTIEGKMCAYGYKDSKGVIK
                     CGIEQAYNDGKISWKKPISCHLYPIKTGMTKFGEVVNYEPRDTMCRPGCLQGKKLSVP
                     VYVFLKEALIRKFGEDFYGVLEQVAESRFADKAEKAEKQKK"
     gene            complement(2328..2912)
                     /locus_tag="VKQ52_05375"
     CDS             complement(2328..2912)
                     /locus_tag="VKQ52_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86643.1"
                     /translation="MRTLLCLLALFLFSCNSVYTPKRRGYFKIDFPPHRYQTFNQPGF
                     PYTFEYPVYANVIRDSSYFDTVPENPWWINIDFPSFGARIYISYKAIGTNKFDRLRDD
                     AFRMTFKHSYKASSIEDSVFTTPNGVGGDYFRVGGNAATAQQFFVTDTTRHFLRGALY
                     FDTAPNEDSLGIVNQFLEKDMDHLINSFRWKTPS"
     gene            complement(2920..4215)
                     /gene="gldE"
                     /locus_tag="VKQ52_05380"
     CDS             complement(2920..4215)
                     /gene="gldE"
                     /locus_tag="VKQ52_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HLZ86644.1"
                     /translation="MELLLINPQATTILAIVILFLLLLSFVLSGSEVAFFTLTYKDLN
                     ILKTKQDVSWKRIVTLLEDPKTLLASLLIANSIINIAIIILANFLIDEMVLLKQNFWL
                     FEFLIKVILIGFVMILFGEVMPKIWATQNNLQFAYYTSGIIELVNLLFRRVSTWAVHQ
                     SETLERFFGSKRPGQALEQLGYQIDKSVKNETSEEERNILKGIVKFGSITVRQVMRAR
                     LDVCGISYDLSFTELKQKIDELHYSRLPVYKSSLDELAGTIQSRDLIGALGEPDNFDW
                     HPYIRPIYFVHEHKLIEDLLQEFQQKKLHFAVVVDEFGGTSGIVTLEDILEEVIGDIR
                     DEFDDEEPDSRRRLDDGSYIFEGRTMLHDVCKTMNLAVDTFDKVKGDSDSLAGLILEL
                     SGDIPKVNDVIPCGDFEFTVLEADSSRIKKVKVTVRMQS"
     gene            complement(4252..4692)
                     /locus_tag="VKQ52_05385"
     CDS             complement(4252..4692)
                     /locus_tag="VKQ52_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLZ86645.1"
                     /translation="MRGVNRVMLIGNLGKDPDVQYLEGNIAVAKFSLATTETFKDRAG
                     KLISQTEWHTVVLWRGLAELAQKYLHKGSLVYIEGRLRTRSWEDKEGNKKFATEVVGD
                     NLIMLDKRTDGPAHPPIHHPEGGVEGIGGTDAAPLGEPSEDLPF"
     gene            complement(4856..5944)
                     /gene="mutY"
                     /locus_tag="VKQ52_05390"
     CDS             complement(4856..5944)
                     /gene="mutY"
                     /locus_tag="VKQ52_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HLZ86646.1"
                     /translation="MTAKKADFTGVLLKWHKGKNHRKMPWKGEKNPYRIWLSEVILQQ
                     TRVQQGWAYYERFLEAFPTIHDLAIAPEQKVFKLWEGLGYYNRCRNLIATAKKIDAEY
                     NGQFPSTYDEILALKGIGPYTAAAIASFAFGLPHAVVDGNVTRVLSRYFGDPTPIDTV
                     GGKKLYTELADALLDKEQADIYNQAIMDFGATICLPRNPLCTDCPQQAHCRAWKDNLT
                     TQLPVKEKSIRKKQRWFYYFIVETPGDKVYIRQRTEKDIWEGLFEFVLWETDEPVYFN
                     NSLPTTASLRQLFGQQSLTVRYISRVYRQELSHQTIQGQFLTVRPDKTLPSLGNYLLV
                     SRRQLPEYAFPKFINAWLLDPTPAQSLF"
     gene            6106..6426
                     /locus_tag="VKQ52_05395"
     CDS             6106..6426
                     /locus_tag="VKQ52_05395"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLZ86647.1"
                     /translation="MRKADLVNQISEKTGIPKVDVLVTLETMFKEVKETLSQGENIYI
                     RGFGSFITKKRAAKIGRNIKKNVAVHIPEHFIPAFKPAKEFVAEVKKLQAVKEDQPNP
                     DEEM"
     gene            6448..7317
                     /locus_tag="VKQ52_05400"
     CDS             6448..7317
                     /locus_tag="VKQ52_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ86648.1"
                     /translation="MKRQQYLVAGTGVLLLLALYFFGQTVPPHSKPDAAASGAPGAPL
                     VKSIGLQEILQASQARLTPAQLSYVNRLQHSVVRGDVQAQQLSANRQLAVFWRDSVAD
                     GFLLYAYYTGEAAKLENSEKSLTFAAQLFLQGLRGQDDPALKSWMATDAKQLFEKALE
                     LNPNNDSTKVGLGASYIFGSAAGSPTEVMQGIQQILAVARRDSTNMYAQLMLGWGGLE
                     SGQFDKAIERLTTVVRHQPANIEATLLLAEVHQQKGNKTDAIRWYEAAKKLLGDRNGE
                     MVKEIDERIKSLR"
BASE COUNT         1666 a         1687 c         2345 g         2043 t
ORIGIN      
        1 tgctttccgg tccttgtatt cgaagcaggt gatgggttgc gggtcgatgc cggtggcgaa
       61 gaggaggccg gtgggggtga tgttcaccgc ctcgtttatc ggtgggtggc cggagtagtt
      121 gatccgggca tcaccggccg cggtaaattc gtcccagtcc gtggtgatcc ggatgggggc
      181 attgaataac tctttggcgc agaaatcgac gatgtatctg agccgcgggg tgttatgggg
      241 gcagaacagg agcatgcaat tgacgggctt cgccggccta gcctttggcg cgttcggcct
      301 tccagcgctg attaaaggtt tgaggagaga attccagttg atcgcgatgg gcggtccagg
      361 ccttgaaggc tttgttgacc atccagttct ttatccgcgg gttgcccata ttcatcctgg
      421 tgcggctgag gcttgccgct ttccagacct tccaggccag tttttccgac caggtggcgt
      481 agccgtcctt tacggcttca tagcgattat cgagcaggag ttcgtggagg ttgatcttta
      541 cggcgcaaac ctcggtgcag ttgccgcaga gggaggaggc atagctcagg tgtttccatt
      601 cgcccatgtc cttcagatgc ggggtgatga cggagccgat ggggccgcta taggtggttc
      661 catagctatg gccgccgata ttcttataga tggggcaggc gttgagacag gctccgcagc
      721 ggatgcagta gaggctttcc cgggcctttg cgttggcgag gatgttcgtg cggccgttgt
      781 cgaggaggat gaggatcatc tcttcggggc cgtcgggttc gcctggctgc cgtgggccgg
      841 tgatgatggt attatagacg gttacgcgct gaccggtgcc ataggtggca agcaggggcc
      901 agaagaggcc gaggtcggtc atggagggga tcactttttc gagcccggcg atgacgatat
      961 gtgtttttgg gaaggatgcg ctgaggcggg cattgccttc gttctcggtg acggcgacgc
     1021 cgccgatgtc ggagatgagg aagttggcgc ccgtgatgcc gatctctgcc tggacatatt
     1081 tttttcggag cttctctctt gccacgaggg tgagctgttc gggggtgagg cccgggcggg
     1141 tgtttagttt ttcgtaaaag agcttggcga cgtcctcctt gcttttgtgc atggcggggg
     1201 tcacgatatg ataggggggt tcgccgtcga gctgctggat atactcgccg aggtcggtct
     1261 cgatgctttc gatgccgttc gcttcgagga agtggtttag ttttatttcc tcggtgacca
     1321 tgcttttgct cttgacgatg gtgcggcagt tcttttcccg gcagatgttg aggatctccg
     1381 cgagggcctg ttcggtgttc tcggcccaga tgacgcggcc gcctctcctt gtgaagtggg
     1441 attcgaactc ttcgagttgc tggtcgaggg tttcgagggc gcgccacttg atattttttc
     1501 cccgttcgcg tgcgaggttc agttcggcga actgttgttt gccctggggc acgacggagt
     1561 tgtagcggcc gatattgaaa ttgatctttt tccgatgttc aagatcttcc gctttgatgg
     1621 tgctccgggc gataaagttg gcggccgttt ctgtcatttc ttctgttttt cggctttttc
     1681 tgctttgtcc gcgaaccggg actctgccac ctgttcgagt acgccgtaga agtcttcgcc
     1741 aaatttgcgg atgagggctt ccttcaggaa gacgtagacg gggacgctga gtttttttcc
     1801 ctgcaggcag ccaggccggc acatcgtgtc gcggggttcg tagttgacca cttccccgaa
     1861 ctttgtcatt cctgtcttta taggataaag atggcagctg atgggctttt tccaggagat
     1921 cttcccgtcg ttgtacgcct gttcgatacc gcacttgatg acccctttgc tgtccttata
     1981 cccgtatgcg cacattttcc cttcgatggt gggggtgacc cagccgaatt cgcggtcata
     2041 ctggtagcgg ccgcgggttt cgatctcttt tatgccggcg ggagtgagat agggttttac
     2101 gatctcgtag gcggcgttta tctcgtcgag ttcggctttt tccagggggg cgccggcgtc
     2161 gccgtcctcg cagcagccgc ccttgcattt gagaagatcg cagacgaact gttcgcggac
     2221 cacgtcgtca cttaccagga tgttgtcgat tgctatcatg atgggtgttg aagggcctcc
     2281 ggcctctttt acaaagatag tggtttgggc gttcgcagga gagggattta ggagggggtt
     2341 ttccagcgga aactattgat cagatgatcc atgtcctttt cgaggaattg gtttacaatg
     2401 ccgagggagt cttcgttcgg cgccgtgtcg aagtagaggg ctccgcggag gaagtggcgg
     2461 gtggtgtcgg tgacgaagaa ttgctgggcc gtggcggcat ttccgccgac cctgaagtag
     2521 tcgcccccta ccccgttggg ggttgtaaag acggagtctt cgatggaact ggccttatag
     2581 gagtgcttaa aggtcatccg gaaggcgtcg tcgcggagcc tgtcgaattt gtttgtgcct
     2641 attgccttgt agctgatata gatacgggcg ccaaaactgg ggaagtcgat gtttatccac
     2701 caggggttct cgggaacggt gtcgaaatag ctgctgtccc tgatgacgtt ggcgtagacg
     2761 gggtattcga aggtataggg gaagccgggt tggttgaaag tctggtatcg atggggcggg
     2821 aagtctattt taaaatagcc tcgtcgtttg ggggtataga cgctgttaca ggaaaagagg
     2881 aagagggcca ggaggcagag tagagtccgc atagtgtgat caggattgca ttcgaacggt
     2941 gactttaact ttttttatcc ggctgctgtc ggcttcgagg acggtgaatt cgaagtctcc
     3001 gcaggggatg acatcgttca cttttgggat atctcccgac aattcgagga tgagccctgc
     3061 caggctgtcg ctatcccctt ttaccttgtc gaaggtatcg acggcgaggt tcatggtctt
     3121 gcagacatcg tggagcatgg tccggccttc gaagatatag ctgccgtcgt cgagccgccg
     3181 gcggctatcg ggttcttcgt cgtcgaactc gtcgcggatg tcgccgatca cctcttccag
     3241 gatatcttcg agcgtaacga taccgctggt gccgccgaat tcatcgacga cgacggcaaa
     3301 gtggagcttt ttctgctgga attcctggag caggtcttcg atgagtttgt gttcgtgtac
     3361 gaaatagatg ggccggatat aggggtgcca gtcgaagttg tcgggttcgc cgagggcgcc
     3421 gatgagatcg cggctttgga tggtgccggc gagctcgtcg aggctgctct tgtagacggg
     3481 caggcgggaa tagtggagct cgtcgatctt ttgttttagt tcggtaaagg agaggtcata
     3541 gctgatgccg cagacatcga gacgggcgcg catcacctgg cggacggtga tgctgccgaa
     3601 tttgacgatg cctttcagga tgttgcgttc ttcttcggag gtttcgttct ttacggattt
     3661 gtcgatctgg tagccaagct gttcgagggc ctggcccggt cttttgctgc cgaagaaacg
     3721 ttcgagggtc tcggactggt gtacggccca ggtgcttacg cggcggaaga gcaggttcac
     3781 caattcgatg atccccgagg tgtagtaggc gaattgcagg ttattctggg tagcccagat
     3841 cttgggcatt acttctccga agaggatcat gacgaagccg atgaggatca cctttatgag
     3901 gaattcgaag agccagaagt tctgtttcag cagcaccatc tcatcgatga ggaagttggc
     3961 gaggatgatg atggcgatat tgatgatgct attggcgatg aggagggagg ccagcagggt
     4021 ctttgggtct tccagcaggg tgacgatgcg tttccaggaa acgtcctgtt ttgtctttag
     4081 tatattgaga tccttgtagg taagggtaaa aaacgctact tccgacccgg aaaggacaaa
     4141 agaaaggagg aggagaaaaa ggatgacgat ggccaggatg gtggtcgcct ggggatttat
     4201 aaggagcaat tccaaaataa tgtgggtttc gttagtaata agctagcaaa actaaaaggg
     4261 caggtcttcg gagggctcgc cgaggggtgc ggcgtcggtt ccgccgatgc cttctacacc
     4321 gccttccggg tgatggatag gcgggtgggc gggtccgtcg gtacgcttgt cgagcataat
     4381 gaggttatct cctactactt cggtggcgaa cttcttgttc ccttccttgt cttcccagct
     4441 gcgggtcctg aggcggcctt cgatatagac gaggcttccc ttgtggaggt acttctgcgc
     4501 cagttcggcc aggccgcgcc agaggacgac ggtatgccat tcggtctggg aaatgagctt
     4561 gccggccctg tctttgaatg tttccgtcgt tgccaatgag aactttgcaa ccgcaatatt
     4621 tccttccagg tattgtacgt caggatcctt tcccagatta cctattagca tcaccctgtt
     4681 tacgcctctc atacgtcgaa ttttgaaatg tttacaaatc gtctttggaa tccagcgccg
     4741 atttaaaatt agcttttttc agccaaaaag ataaattttt ccctcggggt tcattggttt
     4801 ttgagagctg catccccttg acagtccgac gaacacgagc ccctggcgta caaagttaga
     4861 acaagctttg tgccggggtg gggtctagta accatgcatt gatgaatttt ggaaatgcgt
     4921 attcggggag ttgacgccgg ctgacgagca ggtaattccc gagggatggg agtgttttgt
     4981 ccggccggac cgttaggaac tgcccctgta tggtctggtg gcttagttcc tggcgatata
     5041 cccgggagat atacctgact gtgagtgatt gttgaccaaa gagttgccgg agggaggcgg
     5101 tggtgggcag gctattatta aagtagacgg gttcgtcggt ctcccagaga acgaattcga
     5161 acaggccttc ccagatatct ttttccgtgc gttgacggat atagactttg tcgccgggtg
     5221 tttcgacgat gaaataatag aaccagcgtt gttttttgcg aatgcttttt tcttttacag
     5281 ggagttgtgt tgtcaggttg tctttccagg cccggcagtg ggcttgttgc gggcagtcgg
     5341 tgcaaagggg attgcgcggc aggcagatgg ttgcgccgaa atccataatg gcctggttat
     5401 aaatgtctgc ttgttcttta tccagcagtg cgtcagcgag ttcagtatat aattttttgc
     5461 cgccgacggt atcgatgggt gtcgggtcac cgaagtagcg ggagaggacg cgtgtgacgt
     5521 tgccgtctac gaccgcatgt ggaaggccga aggcgaagga ggcgatggct gctgcggtat
     5581 aggggccgat cccttttagt gcgaggatct cgtcgtaagt ggatgggaat tgtccattgt
     5641 attctgcatc aatcttttta gccgtagcga tgagattacg acagcgatta taataaccga
     5701 ggccttccca gagtttgaat actttttgtt ccggggcgat ggcgagatca tgaatagtgg
     5761 gaaaagcttc gagaaatctc tcgtagtagg cccagccctg ttgcacgcgt gtttgttgaa
     5821 ggattacctc gctcaaccag atcctgtaag gattcttttc ccctttccac ggcattttac
     5881 gatgattttt acccttatgc cattttagca aaaccccggt aaaatcggct tttttagcag
     5941 tcattgacga taaataaaat tcacaatcaa tattttagag aattcttgct taaataaagc
     6001 aaatccacta ttttgatacc caaaaagtaa tttcaggcct aatgagttgt gaattaataa
     6061 ttttgttata tatttaaaat cagaaactta taaaacttaa tccccatgag aaaggcagac
     6121 cttgttaacc aaatatcgga aaaaacaggt atacctaagg ttgacgtcct ggtgacgttg
     6181 gaaaccatgt tcaaagaagt gaaagaaacc ctgtcccagg gagagaatat ttatattcgt
     6241 gggttcggga gtttcatcac gaagaagagg gctgccaaga tagggcgcaa tataaagaag
     6301 aatgtggctg ttcacattcc cgagcatttt attcctgcgt tcaagcctgc gaaagagttt
     6361 gttgcggaag taaaaaaatt gcaggcggtg aaggaagatc agccaaaccc ggacgaagag
     6421 atgtaacttg cggtctctaa tcataacatg aagagacagc aatatcttgt agccggcacc
     6481 ggggtgcttt tactacttgc tctgtatttt tttggacaaa ctgttcctcc gcattcgaaa
     6541 ccggacgccg ccgcgagtgg cgctccaggg gctcctcttg ttaaatccat tggtttacag
     6601 gaaattctac aagcgtccca ggctcgtttg acacctgcgc aactatctta tgtaaacagg
     6661 ttgcagcatt ccgtcgtaag gggggatgtg caggcgcagc aattgagtgc gaaccggcag
     6721 ttagcggttt tttggcggga ttccgttgcg gatggcttct tactttatgc ttattacacg
     6781 ggggaagcgg ctaaattgga aaattctgaa aaaagtctca cctttgctgc ccaattattt
     6841 ttgcaaggct tacgggggca agatgatccg gcgttgaaga gttggatggc caccgatgct
     6901 aaacagttgt ttgaaaaggc gttagaattg aatcccaaca atgattcaac caaagtcggt
     6961 cttggtgcga gctatatttt tggaagtgcg gccgggagtc cgacggaggt gatgcagggg
     7021 atccagcaga tcctggcggt agcgcggcgg gattcaacca atatgtatgc tcagctgatg
     7081 ttgggttggg gggggctcga gtcggggcaa tttgataaag ctattgagcg tttgacaacc
     7141 gttgtccggc atcaacctgc caatatcgaa gcgacgctct tactggccga ggtgcaccag
     7201 caaaaaggca acaaaacgga tgccatccgt tggtatgagg ctgcaaaaaa actattaggc
     7261 gaccggaacg gcgagatggt gaaggagatc gatgagcgca tcaaatccct tcgttgagga
     7321 agccgatagg gcttgtaagt tgaaaatcta tttattaact aaaaaatgca ttgagtatgc
     7381 cttgtggtaa aaagcgtaag cgtcataaaa tagcgacgca caagcgtaag aaaagactga
     7441 gaaagaatcg ccataagaag aagaacaaat agttgcttcg gaagaagaac aacttgttgc
     7501 ttcgatggat gtctgaatag cagaggtttg aatgaaaaat ggccgatgat gatggaaaag
     7561 tccgatacgc ttgaataagg aactaatcat aaacgcaact ccgcagggtg tcgaaatagc
     7621 tctactggaa gacaagaagc tggtggagct acaccatgaa aaggctgatg ccagctttgc
     7681 agtcggggat ttataccttg ggaaggtgaa gaagctgatc ccggggttga atgcggcatt
     7741 t
//
LOCUS       DATGFG010000290         4715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_923775, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000290 DATGFG010000000
VERSION     DATGFG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923775"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VKQ52_05405"
     CDS             <1..721
                     /locus_tag="VKQ52_05405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ86649.1"
                     /translation="GAGSGIGKAVAVLLAKQGATVYVADINEEQAGSAVQEIGGNAVA
                     KIADVSDQEQTKALFSSLPRLDILVNSAGLSHIGTAETTQEADMDRLYKVNIKGVYNC
                     IHAGIPVMKKNGGGVIINMCSIAATVGIPDRFAYSMTKGAVYSMTLSVAKDYLKDKIR
                     CNCISPARVHTPFVDGFLKKNYPGKEQEMFEKLSKTQPIGRMAKPEEIAALILYLCSD
                     EASFITGTDYPIDGGFIKLNN"
     gene            726..1583
                     /locus_tag="VKQ52_05410"
     CDS             726..1583
                     /locus_tag="VKQ52_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLZ86650.1"
                     /translation="MQLIRHGQLNKEKPGIILDGKRYDTSAFGEDYTEHFFETDGLRR
                     LRSFIDANKGKLPELAADTRLGSPIARPSKIVCIGLNYSDHAKETGATPPPEPVIFMK
                     STTALIGPNDDIMIPRNSQKTDWEVELAVVIGKKASYVEESEALQYVAGYCLHNDVSE
                     REFQIERSGTWDKGKGCDTFAPMGPWLATSDEINDPHKLRLWLTVNGKKMQDGNTANF
                     IFNIPFLIAYTSQFMTLLPGDVISTGTPAGVGLGMKPNVYLQPGDIVELGIDGLGQAR
                     QNVKAYAAH"
     gene            1570..2418
                     /locus_tag="VKQ52_05415"
     CDS             1570..2418
                     /locus_tag="VKQ52_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ86651.1"
                     /translation="MPHTKTQTIDAHQHFWRFDPIRDSWITEEMRVIKRDFLPADLQP
                     TLQENGIDGCIAVQADQSEDENEFLLAHAAQNDFVKGVVGWVDLRHPDIEQRLQHYTK
                     YPKLKGFRHVLQSETDRALMLRPSFKRGITALRNYGYTYDILIYPDQLGYTRDFVAAF
                     PDQPFILDHIAKPHIRDRYITDEWKDAIHAVAAYPNLYCKISGMVTEADWHHWKPEHF
                     RVYLDTVVEAFGTDRVLYGSDWPVCLVAASYKQVLDIVKDYFTKFSTDEQAAFFGGNA
                     IKFYKL"
     gene            2415..3203
                     /locus_tag="VKQ52_05420"
     CDS             2415..3203
                     /locus_tag="VKQ52_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ86652.1"
                     /translation="MNLELKDKVIIVSGGAKGIGEGIVKVLAAEGAIPFIVGRNEADN
                     KATVSAVEAAGGKAFQTVAELNEPTAAEAAVKTVLDRCGRIDGLVNNAGVNDGVGLEN
                     GSYDAFMSSLHKNLVHYYLLAHYALPALKASRGAIVNIGSKTAETGQGGTSAYAAANG
                     GRSALTREWAVELLKYGIRVNAIIVAECWTPLYERWIQTLPDPKAKLESIVKNIPLGN
                     RMTTSEEIANMTAFLLSGRSSHTTGQLIHIDGGYVHLDRALANA"
     gene            3289..4299
                     /locus_tag="VKQ52_05425"
     CDS             3289..4299
                     /locus_tag="VKQ52_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HLZ86653.1"
                     /translation="MKTLICIQPGEFAYREADAPLARPGEAIIRIRRIGICGTDLHAF
                     EGTQPYFEYPRILGHELSGDLVDFDQAPGFQPGEAVTIIPYFNCGHCIACRNGKPNCC
                     TSIKVCGVHIDGGMVEYLSVPSSSLVHGKNLSLDQLALIEPLAIGAHAVRRAAIRPGE
                     FVLVVGAGPIGLGTMESARIAGAEVIAMDINEDRLRFCREKLQILHTVNPTKDPVQQL
                     QHITRGDMPTVVIDATGSRKAINDAFQYMAHGARYVLVGLQKGTIEFSHPEFHKREGT
                     LMSSRNATREDFDAVMNAMEKGLIDPTTYITHRVNFDQLKSRFPGFLDPANGVIKAMV
                     AL"
     gene            4296..>4715
                     /locus_tag="VKQ52_05430"
     CDS             4296..>4715
                     /locus_tag="VKQ52_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLZ86654.1"
                     /translation="MNKKILSFGELLLRICPGGDWLQDNTLPFFVGGAEANVATALAL
                     WGLPSAYFTALPDNAMTRELAVYLEEKGVDTSRTLYQGERIGLYYLPKGKDLKNAGVI
                     YDRAHSSFSTLRPGTVDWDHVLHDIGWLHFSAISPALN"
BASE COUNT         1144 a         1548 c         1198 g          825 t
ORIGIN      
        1 aggcgccggc agcggcatcg gcaaagcagt agccgtcctg ctggcaaaac agggcgcgac
       61 ggtctatgtc gcggatatca acgaagaaca ggcgggctcc gccgtacagg agatcggcgg
      121 caacgccgtc gcaaagatcg ccgacgtctc cgaccaggaa cagacaaaag cgcttttttc
      181 cagcctgccg cgcctcgata tcctcgtcaa cagcgccggc ctctcccata tcggcacggc
      241 cgaaacaacc caggaggccg atatggatcg cctctacaag gtcaatataa aaggggtcta
      301 caactgtatc cacgccggca tccccgtaat gaagaagaat ggcggcggcg ttatcatcaa
      361 tatgtgctcc atcgccgcca cggtcggcat ccccgaccgc tttgcctatt ccatgacaaa
      421 gggcgccgta tattccatga ccctctcggt ggcaaaggac tatctcaagg ataagatccg
      481 ctgcaattgc atctcgcccg cccgcgtcca tacccccttc gtggatggat tcctgaaaaa
      541 gaactacccg ggaaaagaac aggagatgtt cgagaaatta tcaaagaccc aacccatcgg
      601 gcggatggca aaacccgaag agatcgccgc cctgatcctc tacctttgca gtgacgaagc
      661 ctccttcatc accggaaccg actatcccat cgatggcgga ttcataaaac taaacaacta
      721 gaacaatgca gctcatcaga cacggtcagt taaacaaaga aaagcccggt atcatcctcg
      781 acggaaaacg ttacgacacc tccgccttcg gagaagacta taccgaacat ttcttcgaaa
      841 cggacggcct ccgccgcctc aggagcttta tcgacgccaa caaaggaaaa cttcccgaat
      901 tggcagccga cacccgcctc ggctccccga tcgcccgccc ttcgaagatc gtctgcatcg
      961 ggctcaacta ctccgaccac gcaaaggaga ccggcgccac cccgccgccc gaaccggtca
     1021 tcttcatgaa atcgaccacg gccctcatcg gacccaatga cgatatcatg atcccccgca
     1081 atagccaaaa gacggactgg gaagtcgagc tggccgtcgt catcggcaaa aaggcaagct
     1141 atgtggaaga aagcgaagcc ctgcaatacg tcgccggcta ttgcctccac aacgatgtct
     1201 ccgaacgcga gttccagatc gaacgcagcg gaacatggga taagggcaag ggctgcgata
     1261 ccttcgcccc catgggaccc tggctggcca cctccgacga gataaacgat ccgcacaagc
     1321 tccgcctctg gctcaccgtc aacgggaaaa agatgcagga cggcaacacg gccaacttca
     1381 ttttcaatat ccccttcctc atcgcctata ccagtcaatt catgaccctg ttgcccggcg
     1441 atgtcatctc caccgggaca ccggcgggcg ttggactggg gatgaagccc aatgtctacc
     1501 tccaacccgg cgatatcgtg gagttgggca tcgacgggct gggccaggcc cggcaaaacg
     1561 ttaaagccta tgccgcacac taaaacgcaa accatcgatg cacaccagca tttctggcga
     1621 ttcgatccca tccgggacag ctggatcacc gaggagatgc gcgtcataaa acgcgacttc
     1681 ctgccggccg atctccagcc gaccctgcag gagaacggca tcgacggctg catcgccgtc
     1741 caggccgacc agtccgagga cgagaacgaa tttctcctcg cgcatgccgc acagaacgat
     1801 tttgtaaaag gcgtggtagg ctgggtcgac ctgcgtcatc ccgatatcga acaacggctg
     1861 caacactata caaaataccc gaaactaaag ggcttccgcc atgtcctgca aagcgagacc
     1921 gatcgcgcgc tgatgctcag gccatccttc aaacgcggca tcacggcgct ccggaactac
     1981 ggctatacat acgatattct catctacccc gatcagctcg ggtacacgcg ggattttgtc
     2041 gccgctttcc cggaccagcc cttcatcctc gatcatatcg ccaaacccca tatcagggat
     2101 cggtatatca ccgacgagtg gaaggacgcc atccacgccg tggccgccta ccccaacctg
     2161 tactgcaaga tctcggggat ggtcaccgaa gccgactggc accactggaa acccgaacat
     2221 ttccgggtct atctcgatac cgtggtggag gcttttggaa cggaccgcgt cctctatggc
     2281 tccgactggc cggtctgcct ggtcgccgca agctacaaac aggtactgga catcgtaaaa
     2341 gactatttca ccaaattctc aaccgacgag caagccgcct tcttcggcgg caatgcgata
     2401 aaattctata agctatgaac ctcgaattaa aggataaagt gatcattgtc tccggtggcg
     2461 caaaaggcat cggcgaaggc atcgtaaaag tactggccgc cgaaggcgcc atccccttca
     2521 tcgtcggacg taacgaagcg gacaacaagg caaccgtctc cgccgtcgaa gcagccggcg
     2581 gtaaggcctt ccaaaccgtc gccgagctca acgaaccaac agcggccgaa gccgcggtaa
     2641 agaccgtgct cgatcgctgc ggccggatcg atggcctcgt caacaacgcc ggggtgaacg
     2701 atggggtagg actggagaac ggcagctatg acgcctttat gtcctccctg cacaagaacc
     2761 tcgttcatta ttaccttctc gcccattatg cattgcctgc gctgaaagcc tccaggggcg
     2821 ccatcgtcaa catcggctcg aagacggccg agacaggcca gggcgggaca agcgcctatg
     2881 ccgctgccaa cggcggccgt agcgccctca cccgggagtg ggccgtcgaa ctgctgaaat
     2941 atggcatccg cgtcaacgcc atcatcgtgg ccgaatgctg gacccccctg tacgaacgct
     3001 ggatccagac cctgcccgac ccaaaagcaa aactcgaatc gatcgtaaag aatatcccgc
     3061 tgggaaaccg gatgaccacc tcggaagaga tcgccaacat gacggccttc ctgctctccg
     3121 ggcgaagcag ccatactacc ggtcagctga tccacatcga cggcggttat gttcatctcg
     3181 accgcgcgct cgcaaatgcc tgagccgccg gaggcggctc gtccgaagga cagacattag
     3241 ccaagccgcc ggagccaact cgtccgaagg acaatagtag acaaaaacat gaaaacactt
     3301 atttgcatac agcccggtga attcgcctac cgcgaagcgg acgcgcctct cgcgcgcccc
     3361 ggcgaagcca tcatccggat ccgccgcatc ggcatctgcg gcaccgatct ccatgccttc
     3421 gaaggcaccc aaccgtattt cgaatacccg cgcatcctcg gacacgagct ttccggcgac
     3481 ctcgtcgact tcgaccaggc ccccggcttt cagcccggcg aagcagtaac gatcatcccc
     3541 tactttaact gcggtcactg catcgcctgc cgcaatggca agcccaattg ctgcaccagt
     3601 atcaaggtct gcggcgtcca tatcgacggc ggcatggtgg aatatctctc ggtacccagc
     3661 agctccctcg tccacggaaa aaacctcagc ctcgaccaac tggccctcat cgaacccctg
     3721 gccatcggcg cccatgccgt gcgccgcgcc gccatccgcc cgggagaatt cgtactcgtc
     3781 gtaggcgccg gccccatcgg tctcggaacc atggaatcgg cccgcatcgc cggcgcggaa
     3841 gtgatcgcca tggacatcaa cgaggaccgg ctccggttct gccgcgaaaa actacagatc
     3901 ctccatacgg tgaatcccac aaaggacccg gtgcaacagc tacagcatat cacccgcggc
     3961 gatatgccca ccgtcgtcat cgacgccacc ggcagccgca aggcgataaa cgatgccttt
     4021 cagtatatgg cccacggcgc ccgttatgtg ctcgtcgggc tccagaaagg aaccatagag
     4081 ttcagccatc ccgaattcca caaacgcgaa ggcacgctga tgagcagccg caacgccacg
     4141 cgggaagact tcgacgccgt gatgaacgca atggaaaaag gccttatcga tccgaccacc
     4201 tatatcaccc accgggtcaa cttcgatcag ctaaaatccc ggttccctgg cttcctggat
     4261 cccgccaacg gagtaataaa agcaatggtt gcattatgaa caaaaagatc ctgtcatttg
     4321 gtgaattgct cttacgcatc tgtcccggcg gcgactggct ccaggacaac accctcccat
     4381 tcttcgtcgg cggggcggag gcaaatgtgg ccacggccct ggccctctgg ggtctcccgt
     4441 cggcctattt cacggccctg cccgacaacg ccatgacccg cgaactggcc gtctacctgg
     4501 aagagaaagg tgtcgacacc agccgcaccc tctaccaggg cgaacgcatc gggctctatt
     4561 acctgccaaa aggaaaagac ctgaagaatg ccggcgttat ttacgatcgc gcacattcct
     4621 ctttttccac gctaagaccg ggtacggtgg actgggacca tgtcctgcac gatatcggct
     4681 ggctccactt cagcgccatc tcacccgccc tcaac
//
LOCUS       DATGFG010000291         4041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_397669, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000291 DATGFG010000000
VERSION     DATGFG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397669"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VKQ52_05435"
     CDS             complement(<1..612)
                     /locus_tag="VKQ52_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HLZ86655.1"
                     /translation="MNRIIKKVAVLGSGVMGSRIACHFAGIGLQTLLLDIAPKELTAA
                     ESAKKLTLEHPAVRNRIVNEALAAAIKSNPSPVYTKEVAARIRTGNFTDNMKDIAGCD
                     WVIEVVVERLDVKQQVFAEVEKYRTPGTLITSNTSGIPIHLMAEGRSEDFKKHFCGTH
                     FFNPPRYLRLLEIIPTPYTDEAVTRFLLHYGDLYLGKTTVLCKD"
     gene            complement(740..1378)
                     /locus_tag="VKQ52_05440"
     CDS             complement(740..1378)
                     /locus_tag="VKQ52_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86656.1"
                     /translation="MKQTLQYRILPYNRYNSFYQERESFYRSFINQYYLDFAHQVLSQ
                     EHRDPYLTKTLISPHDIQFGATPRRVIWKIGMPSFQVKDINDFKDHKILFYRTKLLRQ
                     KVLIQFHFINGFFYYSQLSFLTFTEKTNSILCNSIKTKYECQNTMDEQDRLVMTDICN
                     NKLIIEKGVYLTVSYITGDNYPRTLMEQQIKQKHDLVHRADNYEIDRLSKIL"
     gene            complement(1651..2049)
                     /locus_tag="VKQ52_05445"
     CDS             complement(1651..2049)
                     /locus_tag="VKQ52_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86657.1"
                     /translation="MLLIGLIAGLAIYGFLHQIYGGNKALARAEVHITAPELVASFDR
                     DEGQADSLYLYKVISVSGILRELSAKGPGDFVVRLAGDGPGKALVDCHLDSLYNTDDL
                     SLRAGDSVTIRGTCAGRWLNVTLLQCIIEK"
     gene            complement(2158..>4041)
                     /gene="metG"
                     /locus_tag="VKQ52_05450"
     CDS             complement(2158..>4041)
                     /gene="metG"
                     /locus_tag="VKQ52_05450"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HLZ86658.1"
                     /translation="SFDIYHRTSEPLHHETTQEFFTFLNSRGELTVQESEQYYDEEAK
                     TFLADRYIVGTCPVCGNTRAYGDQCENCGTSLSPEMLINPHSTLSGKPPVKKKTSHWY
                     LPLNKHEEFLREWILNEHKDDWRASVVGQCKSWIDGGLQPRAVTRDLDWGIKVPLPEG
                     EGKVLYVWFDAPIGYISATRQWAINTGKDWKPYWYDPETKLVHFIGKDNIVFHCIIFP
                     VMLRLHGNILPDNVPANEFMNLEGDKMSTSRNWKLDMQDYIDDFIKKENGGPRFADVL
                     RYYLTTIAPETKDSEFTWKGFQTANNSELVAKFGNFVNRTFVLMHKLCNGKVPPLHAA
                     RVDETDREMMAAIGATKVRVEELLEAYRFRDAQFEVIRLSELANKYMQTKEPWIVART
                     LAETPGNQQLIDNCLHVCLQLTANLAILINPFLPFTAKKMLHMMKVVEKMLDWENAGK
                     LNLLSVGYSLRAPELLFRPIEDEEVKVQIEKLQAAAAAKKLAEAPAVLQVAGKDGGAG
                     KEPVAAEAPAVKPTIVYDDFAKLDLRVGTILQAEKVEKADKLLKLTVSLGSEQRTIVS
                     GIAAHFKPEEILGRQVVVVANLAPRKMRGIESNGMILMAEDAGRLFFVNPDDRIAPGS
                     GVS"
BASE COUNT          865 a         1096 c         1052 g         1028 t
ORIGIN      
        1 gtccttacac aggaccgtgg tcttgccaag ataaagatcg ccgtagtgca acaggaagcg
       61 ggtcaccgcc tcatcggtat agggggtagg aatgatctcc agcaaccgca ggtaacgtgg
      121 cgggttgaag aagtgcgtcc cgcagaaatg ctttttaaaa tcttcgctcc ggccttccgc
      181 catcagatgg atcgggatac ccgatgtatt ggacgtgatc agggtcccgg gtgtccggta
      241 tttctcgact tcggcaaaca cctgttgttt gacatccagg cgttcgacca ctacttctat
      301 gacccagtcg caacccgcga tatccttcat gttatccgta aaattccctg ttcggatgcg
      361 ggcggccacc tccttcgtat acaccgggga aggattggat ttgatggccg cggcaagggc
      421 ttcgttcacg atccggttcc ggacggccgg atgttccagc gtgagcttct tcgccgattc
      481 tgccgcggtc aactcctttg gcgcgatatc cagcaacagg gtctgcaggc cgatgccggc
      541 aaaatgacag gcgatcctcg aacccatgac cccacttccc aatacggcca ctttcttgat
      601 gatacggttc atatggttaa attagttagt taaacaacta tttacgtcga aggtaacaaa
      661 aaaaaccatt ggccgcgcag cttttcgcaa ccaatgattt cctataatgt gcagcctttg
      721 cggcggggaa tacaaagcgt tacaggatct tgctgaggcg gtcgatctcg tagttgtcgg
      781 cccggtgtac caggtcgtgt ttctgtttga tctgttgctc catcagcgta cgcggatagt
      841 tatcaccggt gatatagctg acggtcaggt atactccctt ctcgatgatc agtttattgt
      901 tgcagatatc cgtcatgacg aggcggtcct gctcatccat cgtgttctgg cattcatact
      961 tggtcttgat ggaattgcac agaatgctat tggtcttttc cgtaaaagta aggaaggaaa
     1021 gctgggagta atagaagaag ccgttgataa agtggaactg gatcaacact ttctgccgca
     1081 gcagttttgt ccggtagaac aggatcttat ggtccttgaa atcgtttatg tccttcacct
     1141 ggaaagaggg catgccgatc ttccagatga cccggcgggg ggtagccccg aactggatat
     1201 catgaggaga gatcaatgtc tttgtgagat aagggtcccg gtgttcctgt gacaacacct
     1261 ggtgagcgaa atcgagatag tattgattga tgaaactacg gtaaaagctc tctctctcct
     1321 ggtagaagga attgtaacgg ttatacggca ggatcctgta ttgaagggtt tgtttcatag
     1381 cacaattcgg tttgtgttag agttgatatt agattttagc gatgcttctt tgtataacat
     1441 tggttacgat ccacgcgaat gttcacgaac caaaaaggac tttttagtca tttttgccag
     1501 tgtacatccg aatatgcttt caatgggaat tgagatcgaa gactccctga agttagcaat
     1561 attgaaacga gattagcacc ccttaagtat ttggattcaa aaatataatt atttgcacct
     1621 agaagatatg tagttcgggt actacagttg ctatttttct attatgcatt gtaagagagt
     1681 gacattgagc catctgcctg cacaggttcc cctgatggta acactatccc ctgcccgaag
     1741 gctgaggtca tccgtgttat aaaggctgtc caggtggcaa tccacaaggg cctttccggg
     1801 cccgtcgccc gccagcctga cgacaaaatc ccccggcccc ttcgccgaca gctcccgcag
     1861 gataccgctc accgagatca ccttatacag gtaaaggcta tctgcctgcc cttcatcccg
     1921 gtcaaaggaa gcgaccagtt caggcgccgt gatatgaacc tccgccctgg ccagggcctt
     1981 gttgccgccg tatatttgat gcaggaaccc gtaaatagcc aggccggcga tgaggccgat
     2041 cagtagcata atgccgtatt tcgtgcgtcg ttccatgtaa gtagtaatca cttacatacg
     2101 aaaaaacggc tccgggggat ttcgcgtctg tcctttgcgg acaagccttt gtccggccta
     2161 gctgacacct gaaccgggtg ctatccgatc gtccgggttg acaaagaaaa gccggccggc
     2221 gtcttccgcc atcaggatca tcccattgct ctcgatcccc cgcatctttc gcggcgcgag
     2281 attggccact accaccacct gcctgcccag gatctcctcc ggcttgaaat gggccgcgat
     2341 acccgagacg atcgtccgtt gttcactgcc cagactgacg gtcagcttga gcagtttatc
     2401 ggctttctcc accttttccg cctgcaggat agtccctacc cggaggtcga gctttgcgaa
     2461 atcatcgtat acgatggtcg gtttgaccgc cggcgcttca gcggcaaccg gctccttgcc
     2521 ggcgccccca tccttgccgg ccacttgcag taccgccggc gcttccgcca gttttttcgc
     2581 cgcggcagcc gcctgcagtt tttcgatctg caccttcact tcctcgtcct cgatcggccg
     2641 gaacaacagc tccggcgccc gcaggctgta accgacgcta agcaggttga gcttgccggc
     2701 attctcccaa tccagcatct tctccaccac cttcatcata tggagcatct ttttggccgt
     2761 aaagggcaga aagggattga tcaggatggc gagattggcc gtcagctgca gacagacatg
     2821 cagacaattg tcgatgagct gctggttccc cggggtctcc gcaagggtgc gggcgacgat
     2881 ccagggctcc ttcgtctgca tatacttgtt ggccagctcg gagagccgga tcacttcgaa
     2941 ctgggcatcg cggaagcgat aggcttccag caactcctcc accctgacct ttgtcgcgcc
     3001 gatggccgcc atcatttccc gatcggtctc gtctacccgg gccgcatgca gcggcggaac
     3061 cttgccattg cacagtttgt gcatcagcac gaaggtcctg ttgacaaaat tgccgaattt
     3121 ggcgacgagt tcgctgttat tcgcggtttg aaaacccttc caggtgaatt cgctgtcctt
     3181 ggtctcgggc gcaatggtcg tcaggtaata ccgcaggaca tccgcaaagc ggggaccgcc
     3241 attctctttt tttataaagt cgtcgatata atcctgcatg tccagcttcc agttgcggct
     3301 ggtgctcatc ttgtcccctt cgaggttcat aaactcgttg gcgggaacat tatccggcag
     3361 gatattccca tgcaaccgca gcatgacggg aaagatgatg cagtggaaaa cgatattgtc
     3421 cttgccgata aaatggacga gtttggtttc cgggtcatac caatagggtt tccagtcctt
     3481 gccggtattg atcgcccact ggcgggtggc gctgatatac ccgatggggg cgtcgaacca
     3541 cacatataat accttaccct ccccctccgg caggggaacc ttgatccccc agtccaggtc
     3601 gcgggttacg gccctgggtt gcagtccgcc gtcgatccag ctcttgcact ggcccacgac
     3661 actggcgcgc cagtcatcct tgtgttcgtt caggatccat tcgcggagga attcttcgtg
     3721 tttgttcaac ggcaggtacc agtgactggt cttcttcttc accggcggct tgccgctaag
     3781 cgtactgtgc gggttgatca gcatttcggg gcttagtgac gtaccgcagt tctcacattg
     3841 gtcgccatat gctctcgtgt tgccacagac ggggcatgtt cctacaatat acctgtccgc
     3901 gaggaaggtc ttcgcctcct cgtcataata ttgttcgctt tcctgaaccg tcagctctcc
     3961 ccggctattc aggaacgtaa agaattcctg cgtggtctcg tggtggagag gctcgctggt
     4021 acggtgatag atatcgaaag a
//
LOCUS       DATGFG010000292         4944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_832449, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000292 DATGFG010000000
VERSION     DATGFG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832449"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2232)
                     /locus_tag="VKQ52_05455"
     CDS             complement(<1..2232)
                     /locus_tag="VKQ52_05455"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HLZ86659.1"
                     /translation="MKDFSFITNSHPSYIEGLYQDYVKNPETVDPEFRKFFEGFDFAV
                     TQGKAATLNGNGAAAANGVTNGSANGVATATTTKSEAAPAAQALMTTDGVDWKKELGA
                     YRLILGYRNKGHLLAKTNPIRPRKDRGANVDLAFYGFTEEDLDKSFFAGNLIGLGTTT
                     LRNILAHIQECYAGHVGIEFKYISDQKKIDWITNEMEKKFLNPLPLEKKRRILEKLNQ
                     GVMFEKFLHTKYIGQKRFSLEGGEATIAALDTIINTAAANSVQEVVIGMAHRGRLNIL
                     ANIMGKTYEQIFSEFEGTAKPDQTMGSGDVKYHMGYGSVVQTDTDKTVYLKLMPNPSH
                     LETVDPVVEGFARAKADILYQSDYDKMLPILIHGDASIAGQGVVYEVLQMSNLKGYYT
                     GGTMHFVINNQIGFTTDFDDARSSDYCTSLAAAIQAPVFHVNGDDAEAVVKCADIATR
                     YRQEFNSDVFIDMVCYRRHGHNEGDDPKFTQPHLYALIDQHPNPREVYTQYLIDHGEA
                     DAKDLAKEMEKKFWGDLQERLDEVKQNPLPYKYQQPEIAWQSLRKATANDFDHSPATG
                     ISEADFKKIFDAIMSWPESFKPLRKVEKLLQDKIKLFNEEGKVDWATSELLAYGSLLL
                     EGKDVRMSGQDVQRGTFSHRHAVLRDENTDEQYNRLSRLPDAKGEFRIYNSLLSEYAV
                     LGFEYGYALANPNALVLWEAQFGDFSNGAQIIIDQFVAAAETKWQRMNGVTLLLPHGY
                     EGQG"
     gene            2672..2935
                     /gene="rpsT"
                     /locus_tag="VKQ52_05460"
     CDS             2672..2935
                     /gene="rpsT"
                     /locus_tag="VKQ52_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HLZ86660.1"
                     /translation="MANHKATKKDVRQAEKRRDRNRYYGKTTRNAIRDLRAIKEQKEA
                     GEKMPEVASMIDKLAKKGVIHKNKAANLKSKLARKVNNLAAAK"
     gene            3046..4761
                     /locus_tag="VKQ52_05465"
     CDS             3046..4761
                     /locus_tag="VKQ52_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HLZ86661.1"
                     /translation="MRKLLPLLFPLFGLAQGPQTPFERSAGRQTTTYAECIAFYKQLD
                     KLSPALSIREMGMSDAGYPYHVVLYSNDGVADPALWHRQHKVVILINNGIHPGEPDGI
                     DASMLLLRDLVAGKAKLPDNVALAVIPLYNIGGALNRTAFSRVNQNGPETYGFRGNAQ
                     NLDLNRDFTKNDSRNARSFVEIFHWVDPVVQIDNHVSDGADYQYTMTLLATQWNKLGG
                     EMGVFLHDVFQPVLFAEMEKKGWPMTPYVEFEEGNPDRGWDGFYDEPRYSSGYAALFH
                     TMAFMPETHMLKPFRDRVLSTYALMQTMIGAASAHGTELLAHRSHDIAADRDSSRLPL
                     DWKVDTTRWDELSFRGYTADEKPSGVTGMPRLYYDRNRPYEKKVRFYDYFASDAAVTV
                     PAAYLIPQGWHEVIDLLKLNGVRMRRIGADTTVSVEVYHIADYKSGSRPYEKHHKNTA
                     IQVRTSREEIRLLRGDYVIPTDQPARRFLVEMLEPTGEDSYFAWNFFDAILQEKEGYS
                     DYRWEDVADQWLKDHPDLRQELEAKRKADPAFAKDARQQLNFVYRHTPYYEPAHMRYP
                     VYRVI"
BASE COUNT         1024 a         1257 c         1516 g         1147 t
ORIGIN      
        1 gccctggcct tcgtagccat ggggcaggag cagggtcacg ccgttcatgc gctgccattt
       61 tgtttccgcg gcggctacga actggtcgat gatgatctgg gcgccgttgc tgaaatcccc
      121 gaactgggct tcccagagaa cgagggcgtt aggattggca agggcatatc catattcgaa
      181 gccgagtacg gcgtattcgc tgagcaatga gttatagatg cggaactcgc cctttgcgtc
      241 ggggaggcga ctgagccggt tgtactgctc gtcggtgttc tcgtcgcgca ggacggcatg
      301 gcgatgagag aaggtgccgc gctggacatc ctgtccgctc atccgcacat cctttccttc
      361 gaggaggagg ctgccatagg ccaggagttc gctggtagcc caatcgacct tgccttcctc
      421 gttgaagagt tttatcttat cctggaggag tttttccacc ttccgcaggg gtttgaagga
      481 ttcgggccag gacatgatgg cgtcgaagat ctttttgaag tccgcttcgc tgatgcccgt
      541 tgccggcgaa tgatcgaagt catttgccgt cgccttgcgc aggctttgcc aggcaatctc
      601 gggttgctgg tatttgtagg gaagcgggtt ttgttttacc tcgtcgaggc gctcctggag
      661 atcgccccag aacttctttt ccatttcctt tgccagatcc ttcgcgtctg cttcgccgtg
      721 atcgatcagg tattgcgtat acacttcccg gggattcggg tgctggtcga tcagcgcata
      781 gagatggggc tgggtgaact tggggtcgtc gccttcgtta tggccatgac ggcggtagca
      841 gaccatgtcg ataaagacgt cggagttgaa ttcctgccgg tagcgggtgg cgatgtcggc
      901 gcatttgacc acggcttcgg cgtcgtcgcc gttgacatgg aacaccgggg cctggatggc
      961 cgcggcgagg gaggtacagt agtccgagct gcgggcatca tcgaaatcgg tggtgaaacc
     1021 gatctggttg ttgatgacaa agtgcatggt tccgcccgta tagtagcctt tgagattgct
     1081 catctgcaat acctcgtata cgaccccctg gccggcgatg gaggcatccc cgtggatcag
     1141 gatggggagc atcttgtcat agtcgctctg gtagaggatg tcggcctttg cgcgggcaaa
     1201 gccttcgacc acggggtcga cggtctcgag gtgggagggg ttaggcatga gtttcaggta
     1261 gacggtcttg tcggtatccg tctgcactac gctgccatag cccatatggt attttacgtc
     1321 gccgctgccc atggtctggt ccggctttgc ggtgccttcg aactcgctga agatctgttc
     1381 ataggtcttg cccatgatat tggcgaggat gttgaggcgg ccgcgatggg ccataccgat
     1441 gacgacctcc tggacggagt tggccgcggc ggtgttgatg atcgtgtcga gggccgcaat
     1501 ggtggcttcc ccgccttcga gggagaagcg tttctggccg atgtatttgg tgtggaggaa
     1561 tttctcgaac atgacgccct ggttgagttt ttccaggatg cgtcttttct tctccagggg
     1621 gagggggttg agaaatttct tctccatttc attggtgatc cagtcgatct ttttctgatc
     1681 gctgatgtat ttgaattcga tgccgacatg gccggcataa cattcctgga tatgagcgag
     1741 gatattccgg agggtggtgg tccccaggcc gatgaggttc ccggcgaaga agcttttgtc
     1801 gaggtcttct tcggtaaagc cgtagaaagc caggtcgacg ttggcgccgc ggtccttacg
     1861 gggacggatg gggtttgtct tggccagcag gtggccttta ttgcgatagc cgaggatgag
     1921 gcggtaggcg ccaagctctt ttttccagtc tacgccgtcg gttgtcatca gggcctgggc
     1981 cgcgggagcg gcttccgact ttgtagttgt tgcggtggca acgccgttgg cggagccgtt
     2041 tgtgaccccg ttggcggcgg cagcgccatt gccgttgagg gtggctgcct tgccctgggt
     2101 caccgcgaaa tcaaagcctt cgaaaaattt cctgaattcc gggtccactg tttcggggtt
     2161 cttcacatag tcctggtaga gaccctcgat gtaactgggg tgggagttgg ttataaatga
     2221 aaagtccttc atagaagaaa tgcgttaagt acaaccgtcc gttttacggt cgaaaatcgg
     2281 gctacaaata tcgctcaaaa atagttgcgg atgggcagga ttttgctggt ttttagtaat
     2341 aatttgctaa ttattaagcc gccgcgggcc ccgcgcgagg gctcggaccg gtagcccgga
     2401 gccgggggac caggcgagga ctatggggga gcgcccggcc cggtagggcg cacccgaaga
     2461 aaagttccga tttatttggc aaatccaggg ctgggaccta cctttgccgt cctttatgag
     2521 ccgctgttgg cccccggatc catagaagag cgagatggac cgagggacga tggtccagcg
     2581 aagctcagac gaaggtcaga cattagccac ttgtgggaaa agggctaatt tttgacaagt
     2641 ccttttccca caacaaaata atgtcgagtt catggcaaat cacaaggcaa ccaaaaaaga
     2701 tgtaaggcaa gccgaaaagc gcagggatcg caaccgctat tacggtaaaa ccacccgtaa
     2761 tgcgatccgt gacctgaggg cgatcaagga gcagaaggaa gcaggggaga aaatgcccga
     2821 agtcgcctcc atgatcgaca aactggccaa gaaaggcgtt atccacaaga acaaagcggc
     2881 caacctgaag agcaaattag cccgcaaggt gaacaatctt gctgctgcaa aatagtgttg
     2941 tgcgggtccc gtgatccggg caacctcctg attagaagca tttacttgta aaatagtttg
     3001 ttaaagaagc attcccgaaa cggaatgctt ctttattttg tggccatgag aaaattacta
     3061 ccgctgctct tcccactttt cggccttgcg caggggccgc agacgccttt cgagcgaagt
     3121 gccgggcggc agaccacaac ctacgccgag tgtatcgcct tctacaaaca actggacaag
     3181 ctctcccccg ccctttccat ccgggaaatg gggatgagcg atgcgggcta tccctaccac
     3241 gtcgtgcttt attccaatga cggggtagcc gatcctgccc tctggcaccg gcagcataag
     3301 gtggtgatcc tcatcaataa cggcatccat cccggggagc ccgacggcat cgacgccagc
     3361 atgctgctgc ttcgtgacct ggtggcggga aaggccaaac tgccggataa tgtggccctg
     3421 gccgttatcc ccctgtacaa tatcggcggg gcactgaacc ggacggcctt ctcccgcgtg
     3481 aaccagaacg gtcccgagac ctatgggttc cggggaaacg cgcagaacct ggacctcaac
     3541 cgcgacttta caaagaacga cagccgcaac gcccggagct tcgtggagat cttccattgg
     3601 gtggacccgg tggtacagat cgataaccac gtcagtgatg gcgctgacta tcaatatact
     3661 atgactttgc tggcgaccca gtggaataag ctggggggcg aaatgggggt gttcctgcac
     3721 gatgttttcc agccggtatt gtttgcggag atggaaaaga agggctggcc gatgacgccc
     3781 tatgtggagt tcgaggaagg gaaccccgac cggggctggg atgggtttta tgacgagccg
     3841 cggtactcca gcggatatgc ggcgctcttc catacgatgg cgttcatgcc ggagacccat
     3901 atgttaaaac cgttcaggga cagggtactg tccacttatg cgttgatgca gacgatgatc
     3961 ggtgcggctt cggcgcatgg gacggagctg ctggctcacc ggagccatga tattgccgcc
     4021 gaccgggaca gcagccggct cccgctcgac tggaaagtgg atacgacacg ctgggatgag
     4081 ctcagtttcc gcggctatac ggcagatgag aagcccagcg gggtgacggg gatgccgagg
     4141 ctttattacg accgtaaccg gccctatgag aagaaagtcc ggttttatga ctatttcgcc
     4201 agcgatgccg ctgttaccgt tcctgcggcc tacctgatcc cccagggctg gcacgaggtg
     4261 atcgatctgc tgaagctgaa cggggtccgg atgcggcgga tcggggccga taccaccgtt
     4321 tcggtggagg tctatcatat tgccgactac aagtccggat cgagacccta tgagaagcat
     4381 cataagaata cggcgatcca ggtcaggacc tcccgggagg agatccggtt gttgcggggg
     4441 gattatgtga tcccgaccga ccagcccgcg aggcgtttcc tggtggagat gctggagcct
     4501 accggcgagg actcctattt cgcctggaat ttcttcgacg ccatcctgca ggagaaggag
     4561 gggtatagcg actaccgctg ggaggatgtg gccgaccaat ggctaaagga tcatccggat
     4621 ctgcggcagg aactggaggc gaaacggaag gccgatccgg cgtttgcgaa ggatgcacgg
     4681 cagcagctga attttgtcta ccggcatacc ccctattatg agccggcgca tatgcggtac
     4741 ccggtgtacc gggttattta ataataaaat tatatataag ccattattaa actactcccc
     4801 ctgccggccg tcttattttc attttaaaac ccatctacta tggcatttac tatgaaaaaa
     4861 gtggctgtct acttgtgcat ttttgtcgga gtcggcttta tttcctcctg tacggtacgg
     4921 gccgttgttc gcgaacggcc tgtg
//
LOCUS       DATGFG010000293         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_69233, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000293 DATGFG010000000
VERSION     DATGFG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69233"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VKQ52_05470"
     CDS             <1..957
                     /locus_tag="VKQ52_05470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HLZ86662.1"
                     /translation="KAIILKAPGGVENLQLAALPQPVPGDREVLVQVKAISINPVDAV
                     VRGDAALLKNILHLREGEQPVILGWDLSGVVTDTGKGVSRFKKGDEVFGMVRFTGHGK
                     AYAEYVTAPEDQVALKPAGVSHEEAAAATLAALTAWQALVTYGHVRKGEKVLIHSAAG
                     GVGSYAVQLARYLGAEVIGTSSAANRDFVLNLGATQHIDYAKEKFEEVVNDADLVLDS
                     VGTPGHLDRSLDALKRGGRLISIVTHFTDETLLKKIREKEVFTHRLGVSSNGEDMWSI
                     AGLMEKDALRSFVTQTFPLEKMAAAHTSIESGRTRGKIVVTV"
     gene            complement(980..1504)
                     /locus_tag="VKQ52_05475"
     CDS             complement(980..1504)
                     /locus_tag="VKQ52_05475"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLZ86663.1"
                     /translation="MIALYIVGGIIVLFLLIAAFVGIAWSFEKSILINAPLEKVWNNV
                     NTLKACNQWNPWMERDPNIKLQYSGTDGTPGASYSWDSPVKNVGAGSQTLTKITPNSE
                     VDSRIDFLRPFKGRGDAYVLLTVERAVTRVSWGIESSTPYPMNILKIFGVIEKNMDRD
                     FGHGLNKLKGLCEN"
     gene            1661..2677
                     /locus_tag="VKQ52_05480"
     CDS             1661..2677
                     /locus_tag="VKQ52_05480"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ86664.1"
                     /translation="MRVTFNYKLTIHILLWSLMLLLPYIVSNSANQYSIGAIPGFLFS
                     AMGFVHIGVFYTHALYIFPRFFNRRFWWVYILSSVLLIWLSLWIKFRMTELWFPAALK
                     RHESYGFIIASSIDIFLISLAYCRILQRIRQEREQKEQQAAQLATELKFLRSQISPHF
                     LFNVLTNLVSLARKKSDQLESSLIMLSELMRYMLYDTQGKKVELRTEIRYLNSYIELQ
                     RLRFGNDVVVESRIDAGEWGEQSAIEPMLLIPFVENAFKHGVGALERPHICIRLSVKE
                     GWMHFEVRNSFDPADTAGKEERSGLGLNNVRTRLDLLYPRDHTLVVREEGNWFHIVLT
                     LKLI"
     gene            2674..3375
                     /locus_tag="VKQ52_05485"
     CDS             2674..3375
                     /locus_tag="VKQ52_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ86665.1"
                     /translation="MIRCMVIDDEQLVRELLEDNIRQIPFLQWVKSCRNALEAAEALQ
                     TTPADLLFLDIQMPRVNGLQFLQSLPDPPLVILVTAYEQYALEGFDLRVVDYLLKPFP
                     FERFLKACNRAQELFRLRQNDRAASAAGVADFFVNVEYALVKIVAADILYVEGVKDYV
                     RIYLSTAARPVLTRMTLKALEEKLEPPAFIRVHKSFLVSVKKITTVKRDLVCIGEKEI
                     PVSVSYKANITRLLK"
     gene            complement(3356..>3677)
                     /locus_tag="VKQ52_05490"
     CDS             complement(3356..>3677)
                     /locus_tag="VKQ52_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ86666.1"
                     /translation="RKGGGEVQLACNGEEAIAFLQKDTSYDLIIMDLQMPIMDGYAAT
                     RFIRKEMLLSTPIIAMTATALVGEQLRCFESGMNDYMTKPFEFTDLYKRITALLTPFI
                     SIAG"
BASE COUNT          777 a          996 c         1076 g          828 t
ORIGIN      
        1 aaagcgatca tcttgaaagc cccgggaggg gttgaaaatc ttcagttggc agcattgccc
       61 caaccggtgc cgggcgaccg ggaggtattg gtacaggtta aggctatcag catcaacccg
      121 gtggatgccg ttgtccgcgg agatgccgcg ctgctcaaaa atatcctgca tttacgcgag
      181 ggcgagcagc ccgtcatcct cggctgggat ctatcgggtg ttgtcacgga tacaggcaaa
      241 ggcgtcagcc gttttaaaaa gggggatgag gtattcggga tggtgcggtt cacgggtcat
      301 ggcaaggcct atgcggaata tgttaccgcc ccggaggatc aggttgcgtt gaagcccgcc
      361 ggggtttccc atgaggaagc cgccgcggct accctggcgg cgctcacggc ctggcaggca
      421 ctggtgacgt atggccatgt ccggaaaggg gagaaggtgc tgatccattc ggccgcgggc
      481 ggggtcggga gttatgccgt ccagcttgcc cgttatctcg gcgcggaggt catcggtacc
      541 agttcggccg ccaacaggga ttttgtgttg aacctcggcg ccacgcagca catcgactat
      601 gccaaagaaa aattcgaaga ggtggtcaac gatgccgatc tcgtgctgga ttcggttggc
      661 acgcccggtc atctcgacag gagcctggat gccctgaaaa ggggcggccg gttgatcagt
      721 atcgtcaccc attttacgga tgagaccctc ctgaaaaaga tcagggaaaa agaagtgttc
      781 acccacaggc ttggcgtcag ctccaatggc gaggacatgt ggtccatcgc gggactgatg
      841 gaaaaagacg cactccggtc tttcgttacc cagaccttcc cgctcgaaaa gatggcggcg
      901 gcgcatacca gcatcgagtc cggccggacg cggggcaaga tcgtggtgac ggtttaaccg
      961 ccgggcggcg ggcgcgggat cagttctcgc agagtccctt tagcttattc agcccgtgac
     1021 caaaatcccg gtccatattt ttttcgataa ccccgaatat cttgaggata ttcatgggat
     1081 agggcgtcga gctctcgatc ccccagctta cccgggtcac cgccctttcg acggtgagca
     1141 atacgtaggc atcgcctctt cccttgaaag ggcgaagaaa atcgatgcgg gaatccacct
     1201 cgctgttggg cgttattttg gtgagggtct ggctgccggc cccgacattc tttacggggc
     1261 tgtcccagga gtagcttgcg cccggagtgc catcggtacc gctgtattgc agtttgatgt
     1321 tggggtcgcg ttccatccag gggttccatt ggttacaggc cttgagcgtg ttgacgttat
     1381 tccacacctt ttccagcgga gcgttgataa ggatggattt ctcaaagctc caggcgatgc
     1441 cgacaaaggc ggcgatgaga aggaagagga cgatgatgcc ccctacaatg taaagtgcga
     1501 tcataaaagc agcaatttat ttccttaaat ataaaaaatt taagggaacg ggacgtctac
     1561 agaatttagg gcgtggtcta cacaccgccc gcgggatgcc gcgaatcaat aatttagcct
     1621 ttattaaccg ctctacccga acttccttag ctttattcga atgagagtta cctttaacta
     1681 taagctgact attcatatcc tcctctggag cctgatgctg ctcctgccct atatcgtttc
     1741 caattccgcc aatcaataca gcatcggggc aattcccggg ttccttttct ccgcgatggg
     1801 gttcgtgcat atcggcgtct tctatacgca tgcgctttat atctttcccc gattcttcaa
     1861 ccggcgtttc tggtgggtgt atatccttag ctctgtcctg ctgatctggc tgtccctgtg
     1921 gatcaaattc cggatgacgg agctctggtt tcccgctgcg ctgaaaagac acgagagcta
     1981 tgggttcatc atcgcctcgt cgatcgatat ctttctgatc agcctggcct attgccggat
     2041 cctgcaacgc atccgccagg agcgggaaca aaaggaacag caggcggcac agctggcgac
     2101 cgaactgaaa ttcctgcggt cgcagatcag cccgcatttc ctgttcaacg tgctgaccaa
     2161 cctggtgtcc ctggccagga agaaatccga tcagctggag tcctcgctga tcatgttgtc
     2221 ggaattgatg cggtatatgc tctatgacac ccagggaaag aaggtggaac tgcggacgga
     2281 gatcaggtat ctcaacagct atatcgaact gcagcggctc cgttttggca acgacgtggt
     2341 cgtggaaagc aggatcgatg ccggggaatg gggggaacag tccgccatcg agccgatgct
     2401 cctgatcccc tttgtcgaga acgccttcaa acacggggtg ggcgcgcttg agcggcccca
     2461 tatctgtatc cggctctcgg taaaggaggg ctggatgcat ttcgaggtgc gcaacagctt
     2521 cgaccccgcg gatacggcag ggaaggagga gcgctcgggc cttgggctta acaatgtccg
     2581 gacccgcctc gatctgttat acccgcgcga ccatacgctt gtcgtgcggg aggagggtaa
     2641 ctggttccat attgtcttaa ctttaaagtt gatatgatac gttgcatggt catcgacgac
     2701 gaacagctgg ttcgtgagct gctggaggac aatatccggc agatcccctt tctgcaatgg
     2761 gtgaagagct gccggaatgc cctcgaagcg gccgaggccc tgcagacgac accggcagac
     2821 ctgctcttcc tggatatcca gatgccgcgg gtcaacgggc tgcagttcct ccaaagcctg
     2881 cccgatccgc cgctggtcat cctcgtcacc gcctatgaac agtacgcatt ggaagggttc
     2941 gatctccggg tggtggatta cctcctgaag ccattcccct tcgaacgctt cctgaaggcc
     3001 tgtaaccggg cccaggagct gttccggctc cggcagaacg accgggcggc gtccgcggcc
     3061 ggcgtcgccg atttctttgt caacgtggag tatgccctcg tgaagatcgt cgccgccgat
     3121 atcctgtatg tcgaaggagt aaaggattat gtcaggatct acctgagcac ggcggcacgg
     3181 ccggtgctga cccggatgac gctcaaggcc ctcgaggaga agctggaacc gccggccttt
     3241 atccgggtgc acaaatcctt tcttgtctcg gtgaagaaga tcaccaccgt gaagcgcgac
     3301 ctggtctgta tcggggaaaa agagatcccg gtgagtgtct cctataaggc caatattacc
     3361 cggctattga aatgaacggc gtgagcaggg ccgtgatcct tttgtacagg tcggtgaact
     3421 cgaagggttt tgtcatatag tcgttcatgc ccgattcgaa acagcgaagc tgttccccta
     3481 ccagggcggt agccgtcatg gcgatgatgg gggtggacag cagcatttcc ttccggatga
     3541 agcgcgtggc cgcatacccg tccatgatgg gcatttgcag gtccataatg atcagatcgt
     3601 aagaggtatc cttttgcaga aaggcgatcg cttcctcccc attgcaggcc agctgcacct
     3661 cgcccccacc cttccgg
//
LOCUS       DATGFG010000294         5094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1077599, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000294 DATGFG010000000
VERSION     DATGFG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077599"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VKQ52_05495"
     CDS             <1..328
                     /locus_tag="VKQ52_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86667.1"
                     /translation="YVDKVVAIGSGGNINKIFSISKRKEGKSLHIDLLKDYHKEFSSF
                     NLEDRMRLYKLREDRADVIVPALLIYINAMRWAGAQEIYVPKIGLADGLIQHLYADLR
                     KKGVEV"
     gene            363..1178
                     /gene="panB"
                     /locus_tag="VKQ52_05500"
     CDS             363..1178
                     /gene="panB"
                     /locus_tag="VKQ52_05500"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HLZ86668.1"
                     /translation="MSVHSDIKKVTTHTLQRMKTNGEKISMITAYDYSFARIFDAAGI
                     DVILVGDSASNVMAGHETTLPITLDQMIYHASSVMRGVNRAFVVVDLPFGSYQSNSKE
                     ALASAIRIMKETGAHGIKCEGGEEILESVKRILAAGIPVIGHLGLTPQSIYKFGTYTV
                     RAREEAEAEKLKKDAALLEEAGCSAIVLEKIPASLAKTVAQSLQIPIIGIGAGHYCDG
                     QVLVMHDLLGINTEFQPRFLRRYLHLHEEITGAVRQYIHDVKASDFPNEQEQY"
     gene            1365..4157
                     /locus_tag="VKQ52_05505"
     CDS             1365..4157
                     /locus_tag="VKQ52_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86669.1"
                     /translation="MNKLAIFVIFLFISVLLHAQKPISGKITDTKTGAPLSGVSVKVK
                     GGKGTSTDNNGLFTITAHPGQTLEISSIGYTPQVQSVTGDFLTITLASASTELTEMVF
                     VGSRGAARSKTESPVPVDIINTGKTSITTARPDLMSQLNMVVPSFNYNKQSGSDGSDA
                     IDFASLRGLGFDQTLVLVNGKRRHLSALVNQYGTRGRGNSGTDLNAIPEAAIDHIEIL
                     RDGASAQYGSDAIAGVMNIVLKKDVHHFNLYTGWSGYYDKKYNALNAVDPTQYYTGSW
                     VDGNTFNLGADGGLALGHNGGFINLAGNFMAQGKTFRAEPDTNVTTNANALPIPAWRR
                     AQGDGSVISGGGMFNMELPIAGSSTTFYSFGGYNYKHSNVYAWTRRWVNSANRIKFPT
                     TPDGNLIFVPSIMHTYHTTDDSISAYNVFYDPQEDVYLKDASFAAGFRGSIGQGWDWD
                     ISNNTGYNDFHYWGNKTFNASLPLPQQATKNRFDDGGFNFLQNTANLDVNKRFAGVAQ
                     GLTLGLGAEFRYERYHLYAGEPDSYRNGGATLPDGTSKASGSEGYPGYQPSDAVEAHR
                     TNVSGYVDLALDVTKAWLVDGAARFENYSDFGFVNTYKLATRYKLADNFNIRGSVSTG
                     FRAPSLQQINFSNTNTVIQNGQLVYAKTVPNYSPLARLAGIPALKQETSVNTSLGFTW
                     QPSRAFTITLDGYIVKMKNRVVATGYFDTTNTSGAVAAYLTANSIAEAQFFANAVNTT
                     NTGLDIVIDYHKTWADKHFTGLLAGNIQHIHIDQVNIPGELNKSASDADAFFSIREQQ
                     FIKASAPAAKFSLALEYGVKKVSFGSHLTYWGKVTTIGGSETVTPDAGGNPITEYFVF
                     GGKVTTDIYSSIKLSKTTSWILGIDNLFNVHPDPAYTKGAKLNSFGDSESGGVFDAVQ
                     MGSNGLHLFSKLVFNF"
     gene            4285..>5094
                     /locus_tag="VKQ52_05510"
     CDS             4285..>5094
                     /locus_tag="VKQ52_05510"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ86670.1"
                     /translation="MKKITVQIVIHIAAWLCFLMLPFVFYPRPSQMDYAFFTERYFNP
                     FFVVNALFIIAFYYCNTYLFIPSLLDRKRFFFYTTLILGLLIIYGLMPRIYHVLFGNI
                     LPPNPALAANRPPGPPRSRPILSAGNIVLFLLVFVFSTGIKVINQWLRSEQRAKEIAN
                     EKLKAELSFLKAQINPHFLFNTLNNIYALASAQSEHTAAAVMKLSSIMRYVLTEARND
                     LVPLEKEIQFTSHYIELQKMRLTDKTSIDFTVQGDPLGRQIAPLLLLPFVEN"
BASE COUNT         1217 a         1712 c         1150 g         1015 t
ORIGIN      
        1 ctatgtcgac aaggtcgtcg ccatcggatc gggcggtaac atcaacaaga tcttctccat
       61 ctccaaacga aaagaaggca aatcactcca tatcgatctg ctcaaggact accataagga
      121 attcagcagc ttcaatctcg aggacaggat gaggctctat aagctgaggg aagaccgcgc
      181 cgacgtgatc gtgcccgccc tactgatcta cataaacgcg atgcgctggg ccggcgccca
      241 ggagatctat gtccccaaga tcggcctcgc cgacgggctc atccagcatc tctatgcgga
      301 tttacgcaag aaaggcgtcg aagtatgaaa aaatgctgct accttttcgc caaaaaatcg
      361 acatgtcagt acactctgat ataaaaaaag taacgaccca tactttacaa cggatgaaga
      421 cgaacggcga gaagatctcc atgatcaccg cctacgacta ctccttcgcc cggatcttcg
      481 acgcggcagg catcgacgtc atcctcgtcg gcgactccgc ctccaatgtc atggccggcc
      541 acgaaacgac cctccccatc accctcgacc agatgatcta ccacgcctcc tccgtcatgc
      601 ggggcgtcaa ccgggccttt gtcgtcgtcg accttccctt cggctcctac cagtccaatt
      661 ccaaggaagc cctcgcctcc gccatccgga taatgaaaga gaccggcgcc cacggcatca
      721 aatgcgaagg gggcgaagag atcctcgagt ccgtaaaaag gatactcgcc gccggtatcc
      781 cggtgatcgg tcacctgggc ctcactccgc aatccatcta taaattcggg acctataccg
      841 ttcgcgcccg cgaagaggcc gaagcagaaa agctcaaaaa agacgccgcc ctgctcgaag
      901 aagccggctg ctccgcgata gtcctggaaa agatccccgc cagcctcgcc aaaaccgtcg
      961 cgcaaagcct ccagatcccc atcatcggca tcggcgccgg ccactactgc gacggccagg
     1021 tcctcgtcat gcacgacctc ctgggcatca ataccgagtt ccaaccccgg ttcctccgcc
     1081 gctacctgca cctccacgag gagatcaccg gcgccgtccg ccaatacatt cacgacgtaa
     1141 aggcaagcga ttttccgaac gaacaggaac aatactagta tatataaata tagttaatag
     1201 ataagaatac ttacgataga agcgtaagtc acttactacc aacgtttgta tgttgtcaat
     1261 gtcatattat aaaaaaatat attaaaaaat agaaataaat cataatgcgt tttttgggtc
     1321 ttttttgcta aattcaatta cgaacaacca aaaccagacg cattatgaac aaattagcaa
     1381 tttttgtcat ctttttgttt atctctgtcc tcctccacgc tcagaagccc atctccggca
     1441 agatcaccga caccaagacc ggcgcccccc tctccggggt ctcggtaaaa gtaaaaggcg
     1501 gaaaaggcac ctccaccgac aacaacggac tctttaccat caccgcccat cccggccaga
     1561 ccctcgagat cagcagcatc ggctataccc cacaggtcca gtccgttacc ggcgactttt
     1621 tgaccatcac cctcgcctcc gcctccaccg agctaacgga gatggtcttc gtcggctccc
     1681 gcggcgccgc ccgcagcaaa acggaatccc ccgttccggt ggacatcatc aataccggca
     1741 agaccagcat caccaccgcc cgccccgacc tgatgtcgca gttgaatatg gtggtgccct
     1801 cttttaatta taataagcaa agcggcagcg acgggtccga cgccatcgac ttcgccagcc
     1861 tccgcggcct gggcttcgac cagaccctcg tactggtgaa cggcaaacgc cgccacctgt
     1921 ccgccctcgt caaccagtac ggtacccgcg gccgcggtaa ctcgggcacc gacctgaacg
     1981 ccattcccga agccgccatc gaccatatcg agatcctccg cgacggcgcc tccgctcaat
     2041 atggctccga tgccatcgcc ggggttatga acatcgtcct gaaaaaggac gtccaccatt
     2101 tcaacctcta caccgggtgg agcggttact acgacaaaaa atacaatgcc ctcaatgctg
     2161 ttgaccccac ccagtactac accggtagct gggtcgacgg aaataccttt aacctgggcg
     2221 ccgacggcgg cctcgccctc ggccacaacg ggggcttcat caacctcgcc ggcaacttca
     2281 tggcacaggg caagaccttc cgcgccgaac ccgataccaa cgtcaccacc aacgccaacg
     2341 cgctacccat ccccgcctgg cgccgggcac agggcgacgg atcggtcatt tccggcggcg
     2401 gcatgttcaa tatggaactg cccatcgccg gcagcagcac caccttctat tccttcggcg
     2461 ggtataatta taaacactcc aacgtctatg cctggacccg tcgctgggtg aacagtgcca
     2521 accggatcaa attccccacc acccccgacg gcaacctcat cttcgtgccg tccatcatgc
     2581 acacctatca taccaccgat gacagcatca gcgcctacaa cgtgttctac gatccccagg
     2641 aagacgtcta tctgaaagac gcctccttcg ccgcaggctt ccgcggcagc atcggccagg
     2701 gctgggactg ggatatcagc aacaacaccg gctacaacga tttccactac tggggcaaca
     2761 agacgttcaa tgcttccctc cccctccctc aacaggcgac caagaaccgg ttcgacgacg
     2821 gcggcttcaa cttcttgcag aatacggcca acctggatgt gaacaagcgc tttgccggcg
     2881 tggctcaggg cctcaccctc ggtctcgggg ctgaattccg ctacgaacgc tatcacctct
     2941 atgccggtga accggactcc tatcgcaacg gtggcgcaac gctgcccgac ggcaccagca
     3001 aggcttcggg ttcggaaggt tacccgggct accaaccttc cgacgccgtg gaagcccacc
     3061 gcaccaatgt cagcggctat gtcgatctcg ccctcgacgt gacaaaggcc tggctggtcg
     3121 acggcgccgc ccgcttcgag aattattccg atttcggctt cgtcaacacc tacaaattgg
     3181 ccacccgcta caaactggcc gacaacttca atatccgcgg ctctgtcagc accggcttcc
     3241 gcgccccctc cctgcaacag atcaatttca gtaataccaa caccgtcatc cagaacgggc
     3301 agctcgttta tgccaagacg gtgcccaatt atagccccct ggcaaggctg gcaggcatcc
     3361 ctgccctcaa acaggagaca tccgtcaaca cttccctggg cttcacctgg cagccttccc
     3421 gggcctttac cattaccctc gacggctata tcgtaaagat gaagaaccgc gtggtggcaa
     3481 cgggctattt cgatacgacc aatacctccg gtgcggtggc ggcttacctg acggccaata
     3541 gtatcgccga agcgcagttc ttcgccaatg cggtcaacac caccaatacc ggtctggata
     3601 tcgtcatcga ttaccacaag acctgggcag acaagcattt caccggcctg ctcgcgggga
     3661 atatccagca tatccacatc gaccaggtca atatccccgg cgaactgaac aaatcggctt
     3721 cggacgcaga cgccttcttc agcatccgcg agcagcagtt catcaaggcc tcggcgccgg
     3781 ccgccaaatt ctccctggcg ctcgaatacg gcgtgaaaaa agtctccttc ggctcgcacc
     3841 tcacctactg gggtaaggta acgaccatcg gcggcagcga gaccgttacg cccgatgccg
     3901 gcggtaaccc catcaccgaa tattttgtgt tcggcggcaa ggtcaccacc gatatctact
     3961 cctccatcaa gttgagcaaa acgacatcct ggatcctggg catcgacaac ctgttcaatg
     4021 tccatcccga tccggcctat accaaggggg caaaactcaa ctccttcggc gactcggaat
     4081 ccggcggcgt attcgatgcc gtacagatgg gcagcaacgg cctgcacctg ttcagcaaac
     4141 tcgtcttcaa cttttaatct ctcaaaaggg caggcgccag cctgcccttt tcttcaccca
     4201 ttcaccggta caatcccccc attcatctaa taattccgtt gatccggcga ttcttggtag
     4261 atttgaaagg ttaataatgc ccttgtgaag aagataaccg ttcagatcgt catccacatc
     4321 gcagcatggc tctgcttcct gatgctgccc ttcgttttct atccgcgtcc cagccagatg
     4381 gactatgcgt tttttaccga acgctatttc aaccccttct tcgtcgtcaa cgccctcttc
     4441 atcatcgcgt tctattattg taacacctac ctgttcatcc cctccctgct cgacaggaag
     4501 cggttctttt tctacaccac gctcatcctt ggtctgctga tcatctatgg cctgatgccc
     4561 cgcatctacc acgtcctgtt cggcaacatt ctccctccca atcccgcgct ggccgccaac
     4621 cgcccgccgg ggccaccccg cagccggccc atcctcagcg ccggtaatat cgtcctcttc
     4681 ctgctggtct tcgtcttcag caccggcata aaagtgatca accagtggct ccgctcggaa
     4741 caacgcgcaa aggagatcgc caacgaaaaa ctaaaggccg aactgagctt cctgaaggcc
     4801 cagatcaacc cccacttcct cttcaatacc ctgaataata tctacgcact ggcgagtgcc
     4861 cagtcggaac acacagcggc agcagtgatg aagctttcca gcatcatgcg ctatgtcctc
     4921 accgaagccc gtaacgatct cgtcccgctg gaaaaagaga tccagttcac gtcacactat
     4981 atcgaactgc aaaagatgcg gctcaccgac aagaccagca tcgacttcac cgtgcaaggc
     5041 gaccccctcg gccgccagat agcacccctg ctgctgctgc ccttcgtcga gaac
//
LOCUS       DATGFG010000295         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_892675, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000295 DATGFG010000000
VERSION     DATGFG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892675"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VKQ52_05515"
     CDS             complement(<1..619)
                     /locus_tag="VKQ52_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ86671.1"
                     /translation="MRLGCTLLFLAAGILCEAQAPMPVLPQAMRSHLREYRAKDDLGG
                     WIYEQIQWVAEAPAGRSALLIHAVDDAWRHPRTEPEVQAWLDLLTNEGYFQLLNGAIV
                     PSTDAYTAAYEWARRHQEVVDPSSLLENILKPLGNNYTRLGDYEQALFIHEKALAIAL
                     AGADKEALAGVYSNLANTCSNMGQPARARDYCRQGLAVADRHSALA"
     gene            complement(629..2749)
                     /locus_tag="VKQ52_05520"
     CDS             complement(629..2749)
                     /locus_tag="VKQ52_05520"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HLZ86672.1"
                     /translation="MRQIKVFFLLLLLATGNACFAQDSIPSEIKIEERGDSMHFSSHL
                     RPLRQIVGAPPCFYSYFWELGDGRFSFDREPVYAYRDTGVYQVRLYATNNYDDGKAPP
                     TRPHPVKIRKKALSRDAWASHFFHGSGEIEMKINRYPRPGENFVTVIGYRNRGSDSLG
                     GSIVLFYNERQLGQEGFALADRRFYNREEGGSLESLMAGLATTETRASLAAGAREGGV
                     EAGRTGSAGSGAGGWAISWRSTDGDRTRDRGWEAEEDREVEGQAAGSVLPDEEDAAAN
                     AAGAQSMLRSLEGTYSQHTVLHFPALGRGEEKYVFVEMNTLPGMLQDTNATVGFSAML
                     VPDDPRVAPEVYQMDMVVVASHDPNRFLFRSRRINYRFMGKKKELSYRVQFQNTGNGP
                     TRRIAIGIGIPPQLDPGSFTLQAVSPACPFCHPGDKSSGCIDTIHRNDSVYIVFNNIY
                     LPGLQQQGIADKDSTEGFVDYTIRFRKKPRKIPFSTQASIVFDHHTPVLTNKATARFI
                     KGLSPGFMAGYGLLPGSGGYSASGPLQFGYVLAPYAPSRPYFQIEAFVGFLQQDAFTS
                     VVVQDQKDTLIAGLPVVVTGRQTKTTTQRNSFEVTPLHYRYNLSNWIGVGAGAMVQVN
                     ISEQTTTENRVYFNTPALPLNIMTAASTQQSAVTYLGSWNAAPFVDVQVGRVRQGPVL
                     GIRYFKLLKGDLTDRFFLYAGFKL"
BASE COUNT          611 a          885 c          719 g          566 t
ORIGIN      
        1 cggccagcgc cgaatgccgg tccgcgacgg ccagcccctg ccggcagtaa tcccgggccc
       61 gcgccggctg acccatattg ctgcaagtat tggcaagatt gctgtagacc ccggccaggg
      121 cttccttgtc cgcgcccgca agggcgatag ccagggcctt ttcatgaatg aacaacgcct
      181 gttcataatc gccaagccgc gtatagttat tccccagcgg cttgaggata ttctccagca
      241 gcgaggacgg gtccaccacc tcctggtgcc ggcgcgccca ctcataggcc gccgtatacg
      301 catcggtgga agggacgatc gcgccgttca gcaactgaaa ataaccctca ttggtaagga
      361 gatcgagcca ggcctgaacc tccggctccg tccgcggatg gcgccaggca tcatcgacgg
      421 catggatcag taacgccgat ctgccggccg gcgcctccgc cacccattgt atctgctcat
      481 agatccatcc gcccagatca tccttcgcac gatactcccg gagatgggat cgcatcgcct
      541 gcggaaggac aggcatcggc gcctgggcct cgcaaagaat gccggccgcc aggaacagca
      601 atgtgcaacc gagcctcata gctgcaaatt acaacttgaa accggcgtaa agaaaaaagc
      661 ggtcggtaag atctcccttc aataatttga aataacggat gcccagcacc ggcccctgcc
      721 gtacccttcc gacctgcaca tcgacaaagg gcgccgcatt ccagctgccc aggtaggtaa
      781 cggcggattg ctgtgtactg gcagcggtca taatgttcaa tggtaaggca ggcgtattga
      841 agtagaccct gttctccgtc gttgtctgct cggagatatt cacctgcacc atggcgccgg
      901 cgccgacacc gatccaattg ctgagattat agcggtaatg cagcggcgtc acctcgaaac
      961 tatttctttg cgtcgttgtt ttcgtctgcc gccccgtgac tacaaccggc aatcccgcga
     1021 tcagggtatc cttttgatcc tgcaccacga cactcgtaaa ggcatcctgt tgcaggaacc
     1081 cgacaaacgc ctcgatctgg aaatagggtc gggaaggggc atagggcgcc aggacataac
     1141 cgaattgcag gggtcccgat gcgctatatc caccgctacc tggtaacagg ccgtaaccgg
     1201 ccataaagcc cggcgaaaga cccttgatga accgcgcggt cgctttattc gtcaacaccg
     1261 gcgtatggtg atcgaatacg atggaagcct gggtgctgaa tgggatcttc ctgggcttct
     1321 tgcggaatcg gatggtgtaa tcgacaaaac cttccgtgga atccttatcc gctataccct
     1381 gttgctgtag ccccggcaga tagatattgt tgaatacgat atagacgctg tcgttccggt
     1441 ggatcgtatc gatacaaccg gaactcttat cccccggatg gcagaaagga catgcggggc
     1501 tcacggcttg taaggtaaaa ctccccgggt cgagctgcgg cgggataccg atgccgatgg
     1561 cgatccttcg tgtcggacca ttcccggtat tctgaaattg cacccggtag gaaagctcct
     1621 ttttcttgcc catgaaacga taattgatcc ggcggcttct gaatagaaat cggttcggat
     1681 catgagacgc caccaccacc atatccatct gatagacctc aggcgccacc cggggatcat
     1741 ccggtaccag catcgcgcta aacccaacgg tggcgttggt atcctggagc atgccgggca
     1801 gtgtattcat ttccacgaac acgtactttt cttcaccccg cccgagggcc gggaaatgca
     1861 gcaccgtatg ctgtgaatac gttccttcca gcgatcgcaa catcgactgt gcacctgccg
     1921 cattggccgc cgcatcctct tcatcgggaa ggacgctccc cgctgcctgg ccctccacct
     1981 cccggtcctc ctccgcctcc cagccccgat ccctcgtccg gtccccatcc gtcgaccgcc
     2041 aactgatcgc ccatcccccg gctccggaac cggcgctccc cgttctcccg gcttcaacgc
     2101 ccccctcccg ggcaccggcg gcaagcgaag cccttgtttc ggtcgtggcc agcccggcca
     2161 tcagggactc cagcgacccc ccttcctccc ggttataaaa tcgccggtcc gcaagcgcaa
     2221 aaccctcctg tcccaactgt cgttcattat agaaaagcac gatcgatccg cccaggctgt
     2281 cactaccccg gttgcgatag ccgatcaccg tcacaaaatt ttccccgggc cggggatacc
     2341 ggttgatctt catttcgatc tccccgctgc catgaaagaa atgagaggcc cacgcatccc
     2401 tgctgagggc cttcttccgg atcttcaccg ggtgaggccg ggtcggcgga gccttcccgt
     2461 catcgtaatt attcgtggca tatagcctta cctggtagac gcccgtatcc cggtacgcat
     2521 acaccggctc ccggtcaaaa ctgaaccggc cgtcgccaag ttcccagaaa taactgtaaa
     2581 aacaaggggg agcgccgacg atctgccgca aaggccggag atgcgaagaa aaatgcatac
     2641 tgtcccccct ttcctcgatc tttatctcgg aggggatcga atcctgcgca aaacaagcat
     2701 tcccggtcgc cagtaacagc aatagaaaga aaacttttat ttgcctcatg aggataaatt
     2761 aacgaaaaaa aagcttctgt a
//
LOCUS       DATGFG010000296         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_601386, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000296 DATGFG010000000
VERSION     DATGFG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601386"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..866)
                     /gene="lipB"
                     /locus_tag="VKQ52_05525"
     CDS             complement(168..866)
                     /gene="lipB"
                     /locus_tag="VKQ52_05525"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HLZ86673.1"
                     /translation="MEKVIFEDLGRMSYQEAWDYQESLLRENVRVKTEAWQQPAAVAP
                     TVHRLLFVEHPPVYTLGKSGHMENVLLDDAAMEERGVEFFRTNRGGDITFHGPGQLVG
                     YPILDLEKFRADLGWYLRSLEEVGIRVLAEYGIRGDRSPGETGIWIDPGIKGRERKIC
                     AMGVRCSRWITMHGFALNVNTDLSWFGNIVPCGIPDKQVTSLEKELGRPVDVEEVKAR
                     WKRQFEAVFGAVLN"
     gene            933..2006
                     /locus_tag="VKQ52_05530"
     CDS             933..2006
                     /locus_tag="VKQ52_05530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLZ86674.1"
                     /translation="MHQRQDNDRELDESPSTDGSEELYERLNLKVDKGQEPLRIDKFL
                     VRRIESASRNKIQKALEAGMVLVNNKQVAPNYKIRPLDEIILYSDKEAHGEEIIPEEM
                     PLNIQYEDEEVMIINKPPGLVVHPASGNYSGTLINGVAWYLLQKNSSLSEQTLPRFGL
                     VHRIDKNTSGLMVLAKTEKAVRSLAKQFFDHTVKRQYMALVWGDLVEESGTIVAHVGR
                     HLRFRKLFDAYPEGDHGKEAITHYKVLERFGYVTLVQCVLETGRTHQIRVHMQHIGHP
                     LFNDETYGGDKIVKGTVFSKYRQFVDNCFATCPRHALHAKTIGFVHPATGKEMLFESE
                     LPEDMKAVIGKWRAYTSNNSLRH"
     gene            2037..2309
                     /locus_tag="VKQ52_05535"
     CDS             2037..2309
                     /locus_tag="VKQ52_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HLZ86675.1"
                     /translation="MPTVKFTYALKRFFPGLKDTPADGTTLAEILGQMETHYPGVRSY
                     LLDEQGHLRKHVNIFIDGTMIKDRASLGDPFPENSEIYIMQALSGG"
     gene            2328..3410
                     /locus_tag="VKQ52_05540"
     CDS             2328..3410
                     /locus_tag="VKQ52_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86676.1"
                     /translation="MRSTLLLGTRKGLVAYQHNGNRWAVENLSFEGQPVSIAYADPRN
                     GNWWACLDHGHWGVKLHRSTNRGQTWEEVAAPAYPEGEEIKDGVPAATRYIWSMAHGG
                     TTRLSKLWVGTDPGGLFMSDDGGANFRLVESLWQHPSRKTGWFGGGRDQPGIHSILVD
                     PRNEDHLHIGVSCAGVFETADGGNNWMVRNTGLKAEFLPDPGSDIGHDPHILVAAPTN
                     PDILWQQNHCGIFRSTDGAKTWQEVSQEGGPARFGFAIAVADDDPDQAWVAPANSDMI
                     RTAIKGALCICRTDDGGKTWREYKKGLPQENCFDIVYRHALASSGEAVAFGTTTGNLF
                     FSPDRGESWETLNNYLPMVYSVQFTD"
     gene            complement(3418..3996)
                     /gene="def"
                     /locus_tag="VKQ52_05545"
     CDS             complement(3418..3996)
                     /gene="def"
                     /locus_tag="VKQ52_05545"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLZ86677.1"
                     /translation="MILPIVAYGTPILRVVSKDITPDYPNLAQLIADMWETMYSSNGV
                     GLAAPQVNRDIRLFVVDSQQIFNNLEEDEKDKYPDGPGIRQVFINAHITALNGEEWSY
                     NEGCLSIPKIREDIVRSEEVTLEYVDENFEPHVSTFNGISARVILHEYDHIEGKLFID
                     YLKPLKRKLLKGKLDDITRGKINVDYKMSFPR"
     gene            complement(4027..>4229)
                     /gene="ruvX"
                     /locus_tag="VKQ52_05550"
     CDS             complement(4027..>4229)
                     /gene="ruvX"
                     /locus_tag="VKQ52_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HLZ86678.1"
                     /translation="REVMKRLQKEFPAIPLKPVDERFTSKLASRAMVEMGMKKKQRRN
                     KALVDEIAATILLQEYLGRAGQ"
BASE COUNT         1026 a         1330 c         1046 g          827 t
ORIGIN      
        1 cctcaccctc gacaaaacaa atttccgcca ttcgggttgg ttccgtttcg aattgtacga
       61 cggcgatcag ttgaaagaaa agaacaaatt cttcattcca aaggaattct aaacgggtct
      121 ccattcaaca ccgcgcgaag cagcacccgg tccaactcca cacccgttca attcaacacc
      181 gccccgaaca cggcttcgaa ctgccgcttc cagcgggcct tcacctcctc tacatccacc
      241 ggccggccaa gctccttctc cagcgacgtc acctgtttat ccgggatgcc gcaagggacg
      301 atattcccaa accaggacag atcggtattg acattcaggg caaatccatg catcgtgatc
      361 cagcggctgc accggacccc catggcgcag atcttccgct cacggccctt gatcccggga
      421 tcgatccaga ttcccgtctc acccggagaa cggtcacccc ggatgccata ttccgccaaa
      481 acccggatac ccacctcttc caggctccgc agataccaac ccaggtcggc acgaaacttc
      541 tccaggtcga gtatcgggta accgaccaac tgccccggtc catgaaaagt aatatcgccg
      601 ccacgattgg tcctgaaaaa ttccaccccg cgttcctcca tcgcggcgtc atccagcaat
      661 acattctcca tatgcccgct cttgccaagc gtataaacag gcggatgctc cacaaacaaa
      721 agacgatgca ccgtcggcgc caccgcagcc ggttgctgcc acgcctccgt ctttacccgg
      781 acattctccc ggagtaagga ctcctggtaa tcccaggcct cctggtagct catccggccc
      841 agatcttcaa agatgacctt ttccatagcc cgcaaagtta ctaaatcgta cctttgccgg
      901 aacgggtcac cggcccgaac aaaatgtctg ccatgcatca gcgacaggat aacgacagag
      961 aattggacga aagcccctcc accgacggct cggaggagct atacgaacgc ctgaatctca
     1021 aggtagataa aggacaggaa cccctgcgca tcgacaaatt cctcgtgcgg cgcatcgaaa
     1081 gcgcctcccg caacaagatc cagaaggccc tcgaggccgg catggtcctg gtcaacaaca
     1141 aacaggtggc gcccaactac aagatcagac ccctcgacga gatcatcctc tactccgata
     1201 aggaagccca tggcgaagag atcatcccgg aagagatgcc cctcaatatc cagtacgagg
     1261 acgaggaagt gatgatcatc aacaaaccgc cgggtctcgt cgtccacccc gccagcggta
     1321 attactccgg aaccctgatc aacggcgtgg cctggtacct gctgcaaaag aacagctccc
     1381 tctccgaaca gaccctgccc cgcttcggcc tggtccatcg catcgataag aacaccagcg
     1441 ggctcatggt gctggcaaag accgaaaaag ccgtccgcag cctggccaaa caattcttcg
     1501 accataccgt caaacgccag tatatggcac tcgtctgggg cgatctcgtg gaagagtccg
     1561 gaaccatcgt cgcgcacgta ggccgtcatc tccgctttcg caaactcttc gacgcctacc
     1621 ccgaaggcga tcacggcaaa gaggccatca cccactataa agtattggaa cgttttggct
     1681 atgtcacgct cgtccagtgc gtgctcgaga cgggccgtac ccaccagatc agggtacaca
     1741 tgcagcatat cggccatccg ctcttcaacg acgagaccta tggcggcgat aagatcgtaa
     1801 aaggcaccgt ctttagcaaa taccggcaat tcgtcgacaa ctgcttcgcc acctgcccgc
     1861 gccacgccct gcatgccaag accatcggct tcgtacaccc ggcaacggga aaagaaatgc
     1921 tcttcgaaag cgaattgcct gaagatatga aagcggttat cgggaaatgg cgggcctata
     1981 ccagcaacaa cagcctccgg cactagacca acccccgctg gctataaaac caggaaatgc
     2041 ccacggtaaa gttcacatac gcgctgaaac gattcttccc cgggctgaaa gacaccccgg
     2101 ccgatggaac gaccctggcg gaaatcctcg gccaaatgga aacgcattat cccggcgtcc
     2161 ggagctatct tctcgacgaa caaggccacc tgcgaaagca tgtcaatatc ttcatcgacg
     2221 gaacaatgat aaaagaccgg gcatccctcg gcgatccgtt cccggagaac agcgagatct
     2281 atatcatgca ggccttatcc ggaggataac cattcaaaac caactatatg cgatccacac
     2341 tcctgcttgg gacccggaag gggctcgtcg cctaccagca taatggaaac cgctgggcgg
     2401 tggaaaacct ttcattcgaa gggcaacccg tctccatcgc ttatgccgat ccgcgcaacg
     2461 gcaactggtg ggcatgcctc gaccacggtc actggggtgt aaaattgcac cggtcgacaa
     2521 acaggggtca aacatgggaa gaagtggccg cccccgccta tcccgaaggg gaagaaataa
     2581 aagacggcgt gccggccgcg acccgctaca tctggtcgat ggcacatggc ggaacgaccc
     2641 gtctgtccaa actgtgggtc ggcaccgatc cgggcggact gtttatgagc gatgacggcg
     2701 gagccaattt ccggctggtg gaatcgctat ggcagcatcc ctcgcgaaag accggctggt
     2761 tcggcggcgg gcgggatcag ccgggcattc attccatcct cgtagacccg cgcaacgaag
     2821 accatctgca catcggcgtc agctgcgcgg gagtatttga aaccgccgac gggggaaaca
     2881 actggatggt gcgaaataca ggcttaaagg ccgaattcct ccccgatccc gggtcggaca
     2941 tcggtcatga tccccatatc ctggtcgcgg cgcccaccaa ccccgatatc ctctggcaac
     3001 agaatcactg cggcatcttc cgctccaccg acggagccaa aacctggcag gaggtaagcc
     3061 aggaaggagg ccccgcccgt ttcggcttcg ccatcgccgt cgccgatgat gatccggacc
     3121 aggcctgggt cgcgccggcc aatagcgata tgatccgtac ggccatcaag ggtgcattat
     3181 gtatctgccg gaccgacgac gggggaaaaa cctggcggga atacaaaaaa ggattgcccc
     3241 aggaaaactg cttcgatatt gtttatcgcc atgcactggc aagctcgggt gaggcagtgg
     3301 ccttcgggac caccaccggc aatctgttct tttcacccga caggggagaa agctgggaaa
     3361 cgctgaataa ctatctgccg atggtctatt cggttcagtt caccgattga ggggatatca
     3421 gcggggaaaa ctcatcttat aatccacatt gatcttcccc cgggtaatat catccagttt
     3481 acccttcagc agtttgcgct tcagcggttt gagatagtcg ataaaaagct tcccttcgat
     3541 gtggtcatat tcatgcagga tgacccttgc ggagatgccg ttaaaggtcg acacatgcgg
     3601 ctcgaaattt tcgtccacat actccagcgt cacttcctcg ctgcggacga tatcctctct
     3661 tatcttgggt atgctcagac agccttcgtt gtaagaccat tcctcaccgt tgagggcggt
     3721 gatatgcgca ttgataaaca cctgtctgat accgggtcca tcgggatact tgtccttctc
     3781 atcctcttcc aggttgttga atatctgctg cgaatccacc acaaaaagcc ggatgtcccg
     3841 gttgacctgg ggcgccgcca gccctacccc gttgctcgaa tacatcgtct cccacatatc
     3901 cgcgatcaac tgggcgaggt tcgggtaatc aggcgtgata tccttgctca ctacccggag
     3961 gatcggcgtg ccatatgcca cgataggtaa aatcatagta tgctcgattg gtaagaattc
     4021 attttgttat tgcccggcac gccccaggta ctcctgcagc aggatcgtag ccgcgatctc
     4081 atccaccagc gccttgttac gccgctgttt cttcttcatc cccatctcca ccatcgcccg
     4141 ggaagcaagc ttggacgtaa accgctcgtc caccggcttc aacgggatcg ccgggaactc
     4201 cttctgcaat cgcttcatca cctcccgta
//
LOCUS       DATGFG010000297         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1188591, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000297 DATGFG010000000
VERSION     DATGFG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188591"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1521
                     /locus_tag="VKQ52_05555"
     CDS             88..1521
                     /locus_tag="VKQ52_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ86679.1"
                     /translation="MPSTFLTLKGVSVQIGGVRILDNLDLTIHRGEQWALTGPSGSGK
                     TVLAHTLAGKHFYTGTITTATRHTSIVEQQHRFRNRPGSTALYYQQRFNASDADQTIT
                     VAQELADAGWDGADAGWLDTLHVRPLLQKPLIQLSNGENKRTQLAIALLEKPELLILD
                     NPFLGLDTEGRATLHTIINDLTAKNIQLLLITSAQELPASITHIARLDQGKWVFQGAI
                     KDFHPTREAATVNLDPAILAQLQQEALDNAGPAFAAAVKMVGTTIRYGEAIILDNINW
                     EVRKGECWNVSGPNGAGKSTLLSLITADNPQAYANEIWLFDRRRGSGESIWDIKKRIG
                     FVSPELHLYFDTGASCFEVVASGLFDTIGLFRMLSTEQEALVMGWMRLLSLQDLASRR
                     LVQLSTGEQRMVLLARALIKNPPMLILDEPCQGLDDEQTTRFRALITTLCEAFGTTLI
                     YVSHYRRELPGCIDHFLRLDRGTIVND"
     gene            1682..1999
                     /locus_tag="VKQ52_05560"
     CDS             1682..1999
                     /locus_tag="VKQ52_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86680.1"
                     /translation="MEFTVLRLIATTIVVAGLFAFTHLKAGSIKGTVSPADGGVRAWA
                     ESATDTLRAPIVNGSYEITDVKPGAYKIIIEAKPPYRNIAKDGILVNDGQAADVGEIK
                     LDK"
     gene            2321..2590
                     /locus_tag="VKQ52_05565"
     CDS             2321..2590
                     /locus_tag="VKQ52_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86681.1"
                     /translation="MYSYALLEKGCYYLIQEKEEGPVGLIKVNMETDHCMYVSKYADA
                     TQALWKKKTDPIFDILELLGDDKVKTWESIYNDSQDTYNYEEDEE"
     gene            complement(2569..3288)
                     /locus_tag="VKQ52_05570"
     CDS             complement(2569..3288)
                     /locus_tag="VKQ52_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86682.1"
                     /translation="MKTGVYSVLYAVVSNLAIILCFVPLLFLSLRKMRQVNTYWVICI
                     YWLFNGLINLPALGLIQSAGGRGFLQRVSYCYTFTETPMVLLAFALAATGRWRKQLLL
                     VLLVFTAAESLLLAWRGYNFTSCSIISGAGLLVVLTYSIAGLWQYLKKMEHSRFENSM
                     VFVYAALLFGYGSFMIIYIFSHYRTGLGGTGYNETDSFLLYYISLLLSASVTSTGLGS
                     YGLRKRRVRRAVVPGYSSSSS"
     gene            3421..>4217
                     /gene="dnaG"
                     /locus_tag="VKQ52_05575"
     CDS             3421..>4217
                     /gene="dnaG"
                     /locus_tag="VKQ52_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HLZ86683.1"
                     /translation="MISQQTIQQILSRIDIIEIVGGFVKLKKRGTNYLGLCPFHNEKT
                     PSFTVSPAKEIYKCFGCGRSGNTISFLMEHEKYSYAEALRWLAARYNVEVEEKELSPE
                     ARQQQQVAESLYIINSFAQKFFSDTLFNTEEGQDIGLSYLKERGFNPEIIRKFQLGYS
                     PDQRDAFARAATAAQYNPEYLQKAGLVVLREEKLIDNYRGRIIFPVHNQTGKVIGFGA
                     RIIRSNDRAPKYINTPENEIYVKSKILYGSYQARQAIDKADECLLVEG"
BASE COUNT         1107 a         1300 c          981 g          829 t
ORIGIN      
        1 cgcaatataa tcaaacgaac gcaacctcca tttatttgaa tttcctcacc tgtggaaaat
       61 tcacccttgc tgtcttacat tcgcgtcatg ccatctactt tcctgaccct caaaggggtc
      121 tccgtacaga tcgggggcgt ccgtatcctc gataatttgg atctgaccat ccaccgcggt
      181 gaacaatggg ccctcaccgg cccctccggc agcggcaaga ccgtcctggc ccataccctc
      241 gccggaaaac atttctacac cggcaccatc actacggcca cccgccatac cagcatcgtc
      301 gaacagcaac accggttcag gaaccggccc ggatcgacgg cactctatta ccagcaacgc
      361 ttcaacgcat ccgacgccga ccagacgatc accgtcgccc aggaactggc cgacgcaggc
      421 tgggacggcg ccgacgccgg ctggctcgat accctccatg tccgccccct cctgcaaaaa
      481 cccctgatcc agctctccaa cggggagaac aaacgtacgc agctggccat cgccctgctg
      541 gaaaaacccg aactcctgat cctcgataac cccttcctcg gactcgacac ggaaggccgc
      601 gccaccctcc ataccatcat caacgacctc accgcaaaga acatccagct cctgctcatt
      661 acctcggcac aggaactgcc cgcctccatc acgcatatcg cccggctcga tcagggcaaa
      721 tgggtattcc agggagctat aaaagatttt caccccaccc gggaagcggc aacggtcaac
      781 ctggaccccg ccatcctcgc ccaattgcaa caggaggccc tggacaacgc cggccccgcc
      841 ttcgccgccg cggtaaaaat ggtcggtacc accatccgct atggcgaagc catcatcctc
      901 gacaatatca attgggaagt gagaaaaggc gaatgctgga acgtctcggg tcccaacggc
      961 gccggcaagt ccaccctgct cagtctcatc accgccgaca atcctcaggc ctacgccaac
     1021 gagatctggc tcttcgaccg ccggcgcggc tcaggggaat ccatctggga tataaaaaaa
     1081 cgcatcggtt ttgtttcgcc cgaactgcac ctgtacttcg ataccggcgc ctcctgcttc
     1141 gaggtcgtcg cctccggcct cttcgatacc atcggcctgt tccggatgct gagcaccgaa
     1201 caggaagccc tcgtcatggg ctggatgcgc ctcctctccc tccaggatct cgccagccgc
     1261 cgcctcgtac aactctccac cggggaacaa cgaatggtcc tgctggcccg tgccctgatc
     1321 aagaacccgc ccatgctcat cctcgacgaa ccctgccagg gcctggatga tgaacagact
     1381 acccgctttc gcgcgctcat tacgaccctc tgcgaagcct tcggcaccac cctcatctat
     1441 gtcagccact accgccggga actgcccggc tgtatcgacc atttcctgcg cctcgacagg
     1501 ggtactatcg tcaacgacta accgaattca tccctcctgc ccggcgcgct gtggaacaat
     1561 ttccccaccc tcgcggcctc gcgcgttctt aacgtttcgt ttatggcaca gtccttgatt
     1621 accatgcagc ggaacccgtc cccaatacgt agaaccattg ttcacattta aatctacgtt
     1681 tatggaattt acagtactca gactgatagc caccaccatc gtagtcgcag gtctgttcgc
     1741 cttcacccac ttaaaagcag gctctataaa aggaacggta agccccgcgg acggcggcgt
     1801 tcgcgcctgg gccgaatccg ccaccgatac cctccgggcg cccatcgtaa atggctcgta
     1861 tgagatcacc gatgtaaaac ccggggccta taagatcatc atcgaagcaa aaccgcccta
     1921 tcggaacatc gccaaggacg gcatcctggt caacgacggc caggccgccg atgtaggcga
     1981 gataaaactg gataaataaa ccaataataa gaggctgtag gataagtggt catgaaagag
     2041 cctggatgct gtcaatcgta tccagggaaa gccattccgg aattcggggt ggctttttta
     2101 ttgattatca acacaatagg gttaatttat catgaacgtc ccccaggggc gggaaagatg
     2161 accgccggga aggccaaaat tcgtacattt gtcttgttgc tgcggaatta ctaatccaac
     2221 ctcagcctct actatccgat ctgatcggac ccacttacta ccccattagt tctgatttca
     2281 ttctttaatg tgtattgtct tatctaaaaa aaggtgatgt atgtattcgt atgcgctgtt
     2341 ggaaaaagga tgttattacc tgattcagga aaaagaggaa ggaccggtcg gcctgatcaa
     2401 agtcaacatg gaaacggacc actgtatgta tgtttctaaa tacgccgatg cgacccaggc
     2461 gttatggaag aaaaagacag atcccatctt cgacatcctt gaattactgg gggatgataa
     2521 agtaaagacc tgggaatcta tttacaatga cagccaggac acctacaatt acgaggaaga
     2581 cgaggaatag ccgggcacga cagccctcct gaccctcctt ttccggagcc catagctccc
     2641 caacccggtg gaagttacgg aagcagacag cagaaggctg atataataca gcaggaagct
     2701 gtctgtttca ttatagccgg ttcccccaag accggtacgg taatgggaaa agatatagat
     2761 gatcatgaaa ctcccatacc caaacaaaag ggcggcatag acaaagacca tggagttctc
     2821 gaaccggcta tgctccatct ttttcaggta ctgccacaaa cccgcgatgg aataagtgag
     2881 cacaaccagc agtcccgcgc ccgaaatgat cgaacaggaa gtgaaattat acccgcgcca
     2941 tgccagcagc agcgactccg cggcagtaaa gaccagcagc accagcaaca actgcttccg
     3001 ccaacgcccg gtagccgcga gcgcaaaagc cagcaacacc atcggcgttt ccgtgaaggt
     3061 gtaacaataa ctcacccgtt gaagaaaccc gcgtccaccc gccgattgga taagacccaa
     3121 tgccggaaga ttgataagcc cattaaaaag ccagtagata cagatcaccc agtacgtatt
     3181 cacctgccgc attttccgaa ggctgagaaa gagaagaggg acaaagcaga ggatgatcgc
     3241 cagattggag accacagcat agagtacaga atagaccccg gttttcaaag gatgtacgtt
     3301 tagttggatg aattacttcg tctcgtttaa aactgaagat aaggataatt tattataggt
     3361 aaaactactc tcttttatac atattttacg gtatttttac gcccacgttc ctacccggaa
     3421 atgatctcgc agcagaccat acaacagatc ctgagccgta tcgatatcat cgagatcgta
     3481 ggcggcttcg taaaattgaa gaaacgcggc accaattacc tcggcctctg ccccttccac
     3541 aacgaaaaga ctccctcctt caccgtctct cctgccaaag agatctataa atgcttcggc
     3601 tgcggccgca gcggaaacac catctccttc ctcatggagc acgagaagta ctcctacgcg
     3661 gaagcgctgc ggtggctcgc cgcccgctac aacgtggaag tggaagaaaa agagctcagc
     3721 cccgaagccc ggcaacaaca gcaggtggcc gaaagcctct atatcatcaa cagcttcgcg
     3781 cagaaattct tttccgatac cctcttcaac accgaagaag gccaggacat cggcctcagc
     3841 tacctgaagg aacgcggctt caacccggag atcatccgca aattccagct gggctacagc
     3901 cccgaccagc gcgacgcctt cgcccgggcg gcaacggcag cccaatacaa cccggaatat
     3961 ctccagaaag ccggtctcgt ggtcctgcgc gaagagaaac tcatcgacaa ctatcgcggc
     4021 cgcatcatct tccccgttca caaccagacc ggtaaggtca tcggcttcgg agcccgcatc
     4081 atccgcagca acgaccgcgc gccgaagtac atcaacacgc ccgagaacga gatctatgtg
     4141 aagagtaaga tcctctatgg atcgtatcag gcaagacagg caatagacaa ggcggatgaa
     4201 tgtctcctcg tggaagg
//
LOCUS       DATGFG010000298         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1545698, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000298 DATGFG010000000
VERSION     DATGFG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545698"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /gene="ygiD"
                     /locus_tag="VKQ52_05580"
     CDS             complement(<1..729)
                     /gene="ygiD"
                     /locus_tag="VKQ52_05580"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HLZ86684.1"
                     /translation="MNLSELNSLTASFEPTAPMPVLFTGHGNPMNAITENSFVAGFRQ
                     AAKEIPKPNAVLCISAHWETRGTQVTAMERPRTIHDFGGFPQELFEVEYPAPGSPELA
                     LETKQLIKSADIGLDTRWGLDHGAWSVIKHFYPQADVPVIEMSLDHTQGPQYHYDLGS
                     ELSALRDKGVLIVGSGNMVHNLRMAAWNRLDEHYGYDWALEANGRMKELILSGDHRAL
                     IDFRQQGRAFDLAIPTPEHYLPLLY"
     gene            complement(829..>2918)
                     /locus_tag="VKQ52_05585"
     CDS             complement(829..>2918)
                     /locus_tag="VKQ52_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ86685.1"
                     /translation="TKGSAADNSFLTLFNYTFPEGDRRTALTQPNAIVISRDLARKLF
                     GTEPALNKTLHVSSSLNGAHDCRITGVFEPNSRPSHTDPDFILSFYGGAIEDRMKRDG
                     ANMAFDNMYTTYALLRPGVDPKRLEAKFPAFIEKYAGNDLRQSGFWRKDFLLAVPDIH
                     LHANMMEMTPSGNIVYCYILGSIAVFVLLLACINFMNLSTARSHKRSSEVGVRKVLGA
                     VRISLIGQFLGESLLMAFIAFLLALGFVALLLPTFEHIAGQSLGLAPGSWMQMIAAFT
                     ALSMLTGLLAGSYPAFYLSSFQPVAILKGRFTNPLAAISLRKGLVVFQFVISVTLIIV
                     TFIISAQLGFLRKADLGFAKDRQIVIPLQSKQARMIYSAFKTGLGGDRRILSVGAGSY
                     YPGIVNASDDNFHKEGQSVSAGQRLRINHVDEDFLRTMDMKMVAGRMFSFDHLASDTL
                     GHVIINEEAVKKIGFASPQDALGRKLLSVYKGQHNSDEIIGVVKDFHYESLHSAITPF
                     VFYLNNSSYYHYAIVHAGGGDMDGILTKLETTWRKLDPGEPFSYSFLDEDFQKNYAAD
                     NRLSGIINGFTVIAILISCLGLFGLTAFSTEQRRKEISIRKVLGARVIGLVLLLSKGF
                     LELVFLAILIAAPLAWLVANRWLQEFTTRVPVGWPVFALTAVIVSAIAFTTISFQAVR
                     TAMSNPVKNLRSE"
BASE COUNT          597 a          770 c          921 g          630 t
ORIGIN      
        1 atataacaag ggcaggtaat gttcgggggt ggggatcgcc aggtcgaagg ccctgccctg
       61 ctgacggaaa tcaataagcg cccggtgatc accggaaagg atcagctcct tcattctccc
      121 gtttgcctcg agggcccagt catagccata gtgctcatcg agcctgttcc aggcagccat
      181 ccgcaggttg tgcaccatgt tcccgctacc tacgatcagc acccccttgt cccgcaaggc
      241 ggagagctcg ctgccgagat cgtagtgata ctgcgggccc tgggtatggt cgaggctcat
      301 ctcgatgacc ggaacatccg cctggggata gaagtgtttt atcacggacc aggccccgtg
      361 atccaggccc cagcgggtat cgaggccgat atccgcgctt ttgatgagtt gttttgtttc
      421 cagcgccagt tcagggcttc ccggcgcggg gtattcgacc tcgaacaatt cctgcgggaa
      481 gccgccgaag tcgtggatcg tcctgggtct ttccattgcc gtgacctggg ttccccgggt
      541 ctcccagtgc gccgagatgc atagtacggc gtttggtttg gggatctctt tggctgcctg
      601 ccggaatccg gcgacaaagc tgttctccgt gatcgcattc atcggattgc catgaccggt
      661 aaacagaacc ggcatcgggg ccgtaggttc gaacgatgcc gtcaggctgt tcagttcgct
      721 aagattcata tgctgttttc ctcggcggaa aggtacgctt tatttttggc gggcggtgct
      781 gacgttcatt cgcgacgagg gccagtcatt agcgggcggc gccggcgttt attcgctccg
      841 caggttctta accggattgc tcatcgccgt gcgcaccgcc tggaagctga tcgtagtgaa
      901 agcgatcgcc gacacgatca ccgctgtcag ggcaaatacc ggccagccta cgggcacccg
      961 ggtggtaaac tcctgcagcc atctgttcgc caccagccag gcaaggggtg ctgcgatcag
     1021 gatcgccagg aacaccagtt ccaggaagcc tttcgacagg aggagcacga ggccgatgac
     1081 ccgggccccc agcaccttgc ggatactgat ctccttcctc cgctgttccg tgctaaaagc
     1141 cgtgagcccg aagagcccca ggcaggaaat gaggatggcg atcacggtaa aaccgttgat
     1201 gatacccgag aggcggttgt cggcggcata attcttttgg aaatcctcgt cgagaaaact
     1261 atagctgaag ggttcgccgg ggtccagctt tcgccatgtg gtctccagtt ttgtcaggat
     1321 cccgtccatg tcgccgccgc cggcatgcac gatggcatag tggtagtaac tgctattgtt
     1381 cagatagaag acaaaggggg tgatggcgga gtggaggctt tcataatgaa agtcctttac
     1441 gacgccgatg atctcatccg agttatgctg tcccttgtat acgctgagca gtttcctgcc
     1501 cagggcatcc tgcggggaag cgaagccgat ctttttaacg gcctcctcgt tgataatgac
     1561 atgacccagc gtgtcggaag ccagatggtc gaaggagaac atcctgccgg ccaccatttt
     1621 catatccatg gttcgcagga aatcttcatc cacatggttg atacgcagcc gctgtcccgc
     1681 gctgacgctt tgtccctcct tatggaagtt gtcgtcgctg gcgttgacga tgccgggata
     1741 gtaggagccc gcgcccacgg acaggatccg ccggtcgccg cctaatccgg ttttgaaagc
     1801 ggagtagatc atccttgcct gtttgctttg gagcgggatg acgatctgcc ggtctttggc
     1861 gaagcccagg tcggccttcc ggagaaagcc cagttgggcg gagatgatga aggtgacgat
     1921 gatcagggta acggagatca cgaactggaa taccaccagt cctttgcgca gggagatcgc
     1981 ggcaagggga ttggtgaacc ggcctttcag gatggctacc ggctggaagg aggataaata
     2041 gaaggcggga tagctgcccg cgaggaggcc ggtgagcatt gataatgcgg tgaaggccgc
     2101 gatcatttgc atccatgatc ccggggccag gccgagggac tgaccggcga tgtgctcaaa
     2161 ggtgggcaag aggagggcga cgaacccaag cgcgaggagg aaggcgatga aggccatcaa
     2221 cagggactcg cccaggaact ggccgatcag ggaaatcctt accgctccca ataccttgcg
     2281 gacacctact tccgaagagc gtttgtgcga gcgggcggtg gagaggttca tgaagttgat
     2341 acaggccagc aggaggacga acaccgcgat ggagccaagg atataacaat agacgatatt
     2401 cccggaaggg gtcatctcca tcatattggc atgcaggtgg atgtcgggga cggccaggag
     2461 aaaatccttg cgccagaagc cagattgccg gagatcgttg ccggcgtatt tttcgatgaa
     2521 ggcggggaat ttcgcttcga gccgttttgg gtccacgccg ggccttaaca gcgcataggt
     2581 ggtgtacatg ttgtcaaagg ccatgttggc gccgtcgcgt ttcatccggt cttcgatggc
     2641 gcctccgtag aaggaaagga tgaaatccgg atcggtgtga gagggccggc tattgggttc
     2701 gaacacgccc gtgatgcggc agtcatgggc cccgttgagg ctgctgctga catggagtgt
     2761 tttgttcagt gcgggttccg tcccgaagag cttgcgggcg agatcccgtg aaattactat
     2821 agcgtttggc tgggtcagcg ccgtgcggcg atcgccttcg gggaaggtat agttgaacag
     2881 ggtgagaaag ctgttgtcgg ccgcgctgcc ctttgttt
//
LOCUS       DATGFG010000299         6548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1552521, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000299 DATGFG010000000
VERSION     DATGFG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552521"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1424)
                     /gene="dinB"
                     /locus_tag="VKQ52_05590"
     CDS             complement(258..1424)
                     /gene="dinB"
                     /locus_tag="VKQ52_05590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HLZ86686.1"
                     /translation="MSTEQRHIAHFDLDAFFVSVECLKNSSLKGKPLLVGGAGDRGVV
                     AACSYEARKFGIHSAMPMKLARRLCPEAIIIRGDMESYSKYSRLITEIMQDNVPLFEK
                     ASIDEFYIDLSGMDKFFGCSLFARELKTRVTKESGLPVSYALASNKLVSKVATNEVKP
                     NGQLEIAFGGEKTFLAPLSVSKLPGIGKETAYKLIRRGVETIRTLSNIPIEMLGTMLG
                     KNGIDLWRKANGLDESPIIPYREQKSISTEQTFETDTINLGFLHAQLVRMTESIGFDL
                     RRQDKLTGCVTVKLRYSNFDTVARQKTIDYTNADHILLTTAQELFDKLYDRRMLIRLV
                     GIRFTRLVPGNYQIKLYEDTQEMIRLYQSIDSIKRRYGEKAVLRAVGAKRGQEK"
     gene            complement(1426..4395)
                     /gene="dnaE"
                     /locus_tag="VKQ52_05595"
     CDS             complement(1426..4395)
                     /gene="dnaE"
                     /locus_tag="VKQ52_05595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HLZ86687.1"
                     /translation="MFPQDLTMYLCKSYFSFNYGTFSTEELVAAGVEAGATTLILTNI
                     NATCDAWDFVLFCGKQNIKPILGAEIRNGNTLLYILIAANNKGFRRINEFLSEHLREK
                     KPFPPGDALSSVFPAPEDGFIIHPLGSKAPGLLDSNERIGVLPGEVNKLLKQPLASFP
                     DKWVIRQPVTFQNKTYYNLHRLLRAIDGNTLLTKLTPEDLASPQEYFIPQGEMLTAFR
                     QYPGLITNTYRLLDACSITMDFGVDKNKQLFSASREDDRVLLEKLARDGFRFRYGANK
                     KIKERFNKELRIIDQMGFTAYFLITWDMIRYAQSRGFYHVGRGSGANSLVAYCLQITD
                     VDPIELDLYFERFLNPHRTSPPDFDIDFSYLDRDEIIDYMMKRYGRNHTTLLGMYPTF
                     QYNAIIRELGKVFGLPKAEIDSLAARGSSGDDKIHRAIVQYGKLLRNFPSALSIHPGG
                     ILISEKPLASYCAIHLPPKGFPTAMIDMFVAENIGLYKLDVLSQRGLGHIKECIQLVK
                     TNHRIDINIHEIDKFKKDPAIKEQLRTGNTIGCFYIESPAMRQLIKKLKCDNYETLVA
                     ASSIIRPGVGRSGMMYQYIYRYNNPGKFEYLHPRLKDILGSTYGVMIYQEQVIQVAHE
                     WAGLEMADADLLRRATSSKYRGKGHLPMLKEKFFANCVGFGYPAEAVEEVWRQIESFA
                     DYSFCKAHSASYAVESYQSLFLKTHFPMEFAVAVINNFGGFYSRELYFYELMRAGARV
                     HRPCVNNSDYYTNLHGMEAYVGFVHIKGLEQAFAARIIEARETAGAYLHLADFIERTA
                     VTPEQLDILIRTGALRFTGKSKKELLWEGDFLQKKTRLSSPVPRPLFKEAPISFTLPD
                     LPSYPMEDYYDDIELLGFPVADPFVLVDDDPSRYLTARDLTSHLGRTITVLGYHITHK
                     PVTTVKGDTMSFGTFIDSNKEWIDTVHFPPIHAAHPPQAGFFRITGKVIEEFGAYTIE
                     VTRIEKAGIRTRTPQTV"
     gene            4624..4911
                     /locus_tag="VKQ52_05600"
     CDS             4624..4911
                     /locus_tag="VKQ52_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86688.1"
                     /translation="MGTRDPRYDLIKPMLSEGQIESFTDVFKYIKKTVVANDLGKKVD
                     RFTIMMEQPKKFSVGDVYEMADLFNIDVRQMFELIYQENLKKAHDEEELSS"
     gene            5204..>6548
                     /locus_tag="VKQ52_05605"
     CDS             5204..>6548
                     /locus_tag="VKQ52_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86689.1"
                     /translation="MKPIFTLCLLFLARWISAQTPVTAQFAHWNTASVSTTYSGTGAT
                     GNVGSGLTGDTYTYEFGPVVTTPNLYILDSFTAVGLNFHFAAPSVVKFRRVDNASVTG
                     LRKSLWFEQNSATTVANGGIVAFRPPYDDSLERIFSSGQIFNIGIDNDFENSTNTNNN
                     NIERVDHIIGGGVSATDGTKTGFVVFDRGNGGGHDPFYIAAIKTLDASGNPSSYYNAV
                     SAGASNYGSDVSPAMNYITLRKNPADTRLLMMNNSATQYRDGVLLRFTDLGVPNGATI
                     YGYSLFGMDVNVSSAANLVDYTNATNFPTGSDYANGGLDQVAVTGLWVTNASYVVLAD
                     RVTDLSAKVVGGKVQLNWALGVMDDLGRLVVERSADGVHFNSLLEFSAPLAGTQTVWD
                     EQPLSGQDDYRLKLVDQRGEVVAYSMVSRVAVSPASAGEAFTVYPNPVAGRQLRFS"
BASE COUNT         1336 a         1591 c         1948 g         1673 t
ORIGIN      
        1 ggggttttcg atcaattcct tgaagcggtt cttctccttt cccagatcgg ttgcgactac
       61 ggttatcggg accagtttga atttaaagat ctcactgagc atcgttagct ttccgcttgt
      121 gaacatcggt ctaacgagat catacccatt atgcttcgcc attatcaaaa tacgtggtta
      181 ttgcttattt gaactaccgc cgaagtatag gtttgacttt tcatgtcccg actattgacc
      241 ttttgcatcc cattttatta cttttcctgt ccccgtttgg ccccaacggc ccgcagaacg
      301 gccttctcgc catagcgccg tttgatggag tcgatggatt gatagagccg gatcatctcc
      361 tgggtgtcct cgtagagttt gatctggtaa ttgcccggca ccaggcgggt gaagcggatg
      421 cccaccaggc ggatcagcat ccggcgatcg tagagtttgt cgaagagttc ctgggcggtc
      481 gttagcagga tgtggtcggc gttggtgtag tcgatggtct tttgtctggc cacggtgtcg
      541 aagttggaat accggagttt gacggtcacg cagccggtga gcttgtcctg ccggcgaagg
      601 tcgaagccga tggattcggt catcctgacc agttgggcat ggaggaagcc gagattgatg
      661 gtgtcggtct cgaaggtctg ttcggtggag atggattttt gttcccggta ggggatgatg
      721 ggggattcat ccaggccatt ggcttttcgc cagaggtcga taccgttctt tcccagcatg
      781 gtgcccagca tttcgatggg gatattgctc agcgttctta tggtctccac tccccgccgg
      841 atcagtttgt aggcggtttc ttttccgatg ccgggcagtt ttgagacgga gaggggcgcc
      901 agaaaggttt tttctccgcc aaaggcgatc tccagttgtc cgtttggctt tacttcgttt
      961 gtggccacct tgctgacgag cttgttgctg gcgagggcat aggatacggg caggccgctt
     1021 tctttggtca cccttgtctt tagctcccgg gcaaagaggc tgcacccgaa gaacttgtcc
     1081 atcccggaca ggtcgatata gaactcgtcg atcgaggctt tttcgaacag ggggacattg
     1141 tcctgcatga tctccgtgat cagccgggaa tatttcgagt aggactccat atcgccgcgg
     1201 atgatgatgg cttcggggca gagacgccgg gcgagtttca tcggcatggc gctgtggatg
     1261 ccgaatttcc gggcctcata gctacaggcc gcgacgacgc cgcggtcgcc ggcgccgccc
     1321 accagcaggg gcttgccttt caggctggaa ttcttcaggc attccaccga tacgaagaag
     1381 gcatcgagat cgaaatgggc gatgtggcgt tgttcggtgg acatgtcaaa cggtttgggg
     1441 ggtgcgtgtt cttatgcctg ctttttcgat gcgggtgact tcgatggtgt atgccccgaa
     1501 ttcttcgatc acttttcctg tgatgcggaa aaagccggcc tgtggcgggt gggcggcatg
     1561 gatcgggggg aagtgtacgg tgtcgatcca ttccttattg ctgtcgatga acgtgccgaa
     1621 gctcatcgta tcgcccttta ccgttgtcac gggcttatgg gtgatatgat aaccgaggac
     1681 ggttatcgtc cggcccagat ggctggtcag gtcgcgggcg gtgaggtacc gggacggatc
     1741 atcgtcgacc agcacaaagg ggtcggcgac ggggaatccc agcaattcga tatcatcata
     1801 ataatcttcc atcgggtagg aggggagatc gggcagggta aaggagatgg gggcttcttt
     1861 gaagagcgga cggggcaccg ggctgctgag ccttgtcttc ttttgcagga aatccccttc
     1921 ccacaacagc tccttcttgc ttttcccggt gaagcggagg gcgccggtcc ggatgaggat
     1981 gtccagttgc tccggtgtga cggcggtgcg ttcgatgaaa tcggccaggt ggaggtaggc
     2041 gccggcggtc tctcttgctt cgatgatcct tgcagcaaag gcctgttcca gcccttttat
     2101 atggacgaag cccacatagg cttccatccc gtggaggttg gtgtagtagt cgctgttgtt
     2161 cacacagggc cggtgtacgc gggcgcccgc cctcatcagc tcatagaaat acaactcccg
     2221 gctatagaac ccgccgaaat tgttgatgac ggctacggcg aattccatgg gaaaatgggt
     2281 cttcaggaaa aggctctgat aactctccac ggcataactc gcggagtgcg ccttgcagaa
     2341 gctatagtcg gcgaaacttt cgatctgccg ccagacctcc tccactgcct ctgccgggta
     2401 accgaatccc acgcagttgg cgaagaattt ctctttcaac atgggcaggt gtcctttccc
     2461 gcgatacttg gaactggtgg cgcgccgcag gagatcggca tcggccattt ccagtcccgc
     2521 ccattcatgg gctacctgga tgacctgttc ctggtagatc atgaccccat aggttgaccc
     2581 caaaatatct tttagcctcg ggtgcaggta ttcgaatttg ccggggttat tataccggta
     2641 gatgtactga tacatcatcc cgctgcgtcc tacacccggc cggatgatcg agcttgccgc
     2701 cacgagggtc tcatagttgt cgcatttcaa tttctttatc agttgccgca tcgcggggga
     2761 ttcgatatag aagcagccga tggtattgcc ggtgcggagc tgttccttta tggccgggtc
     2821 ttttttgaac ttatctatct catggatatt gatatcgatg cgatgattgg tctttaccag
     2881 ctgtatacat tcctttatat gtcccagccc gcgctggctc agcacgtcca atttatagag
     2941 cccgatattc tccgcgacga acatatcgat catggcggtt gggaaaccct ttggcgggag
     3001 gtggatggcg cagtagctgg ccagcggttt ttccgagatg aggatgccgc cggggtggat
     3061 gctcagtgcg ctggggaagt tccggaggag tttgccgtat tggacgatgg cgcgatggat
     3121 cttgtcgtcg ccggacgagc cccttgccgc gaggctgtcg atctcggctt tggggaggcc
     3181 aaacaccttc cccagttccc ggatgatggc gttgtactgg aaggtgggat acatgcccag
     3241 cagggtggta tggttccggc cataccgctt catcatgtag tcgatgatct catcgcggtc
     3301 gagataggag aaatcgatgt cgaagtcggg tgggcttgtc cggtgggggt tcaggaagcg
     3361 ttcgaaatag aggtccagct cgatggggtc cacatcggtg atctggagac agtaggctac
     3421 gagggagttg gcgccgctac cgcggccgac gtgatagaaa ccccggctct gggcatagcg
     3481 gatcatatcc caggtgatga ggaaataggc cgtgaaaccc atctggtcga tgatacggag
     3541 ctctttattg aaccgttcct ttatcttctt attggcgcca tagcggaaac ggaatccgtc
     3601 gcgggccagt ttttccagca gcacccggtc gtcttcccgg ctggcgctga agagctgttt
     3661 gttcttgtcg accccgaaat ccatggttat cgagcaggcg tcgaggagcc ggtaggtgtt
     3721 ggtgatgagg ccgggatatt gccggaaggc ggtgagcatc tccccctgcg ggatgaagta
     3781 ttcctgcggg gatgccaggt cttccggggt cagttttgtc aggagggtgt tgccatcgat
     3841 cgcccggagg agccggtgaa gattataata cgtcttattc tggaaggtca cgggctggcg
     3901 gatgacccat ttgtccggga aggaggccag gggctgcttc aataatttgt tcacttctcc
     3961 gggaaggacc ccgatgcgtt cattgctgtc cagcaggccg ggtgctttgc tgccgagcgg
     4021 gtggatgatg aacccatcct cgggtgcggg gaatacggag cttagcgcat cgccgggtgg
     4081 gaagggtttt ttctcccgga ggtgttcgga gaggaattcg ttgatccggc ggaaaccctt
     4141 gttattggcg gcgatcaata tatataaaag ggtattgccg ttgcggatct ccgcgccgag
     4201 aatgggtttg atgttctgtt tgccgcaaaa caggacaaaa tcccaggcat cgcaggtggc
     4261 attgatgttg gtgagtatta atgtagtagc gcctgcttct acgcctgccg ccactaattc
     4321 ttctgttgag aacgtcccgt agttgaaact gaaataggat ttgcaaaggt acatggtaag
     4381 gtcttgtgga aacaaaacta atatttttag ttctctaatt actaaaatta ttagtaaatt
     4441 tgttttgttg aaatgctttg tgatcctttg ctgcagcggg tttgagcaaa aatgacaaaa
     4501 gggtagtttg tgaaagactg atcggggtgg ggaagatgaa caactggggt ataaaaaagg
     4561 acaacacaaa aaaatagttt tttgtatgcc gttatctaat tcaaaaaaaa cttttctttg
     4621 attatgggaa cgagagaccc gcgttatgac cttatcaaac ccatgctgtc cgaaggtcag
     4681 attgaatctt ttactgacgt ctttaaatac atcaagaaaa ccgttgttgc caatgacctt
     4741 gggaagaagg tagacaggtt tactatcatg atggagcagc cgaagaaatt ctctgtcggg
     4801 gatgtttatg agatggctga cctgttcaat atcgatgtgc ggcagatgtt cgagctcatc
     4861 taccaggaga acctgaaaaa agctcacgat gaagaggagt tgtcgtcctg agttccataa
     4921 ccattagctg tcgagccctt atccattttt accagtctcg acccgaatcc agcaatcttt
     4981 cgtacaggcc gccgcgggta accgcggcgg ccgttgtttt tagccgttgg acccgtggcg
     5041 gtccaatttt gtccagcgaa ctctggggaa gcccccatcc ccctgttaat ggttattatt
     5101 taacccatgg gtgaaaagcg atcgttttct ttcacgaacc acatcagggc agtatttttc
     5161 cgaagcgggc ctcctcaggg agcccgatca aacacccaaa tccatgaaac cgatctttac
     5221 cctgtgttta ttattccttg cgcgatggat atcagcgcag acgccggtaa ccgcgcaatt
     5281 tgcccattgg aatacggcgt ccgtctccac cacgtattcc ggtacggggg ccacgggcaa
     5341 tgtgggaagc gggcttaccg gagataccta tacgtatgaa ttcggtcccg tggtgaccac
     5401 tcccaatctt tatatcctgg attcttttac ggcggtgggc ctgaactttc atttcgcggc
     5461 tccctccgtg gtgaagtttc gccgggtgga caatgcgtct gtaacggggt tgcggaaatc
     5521 gctctggttc gagcagaaca gcgcaaccac ggtggccaat gggggtattg tcgcgtttcg
     5581 ccctccttat gacgacagtc tcgagcgtat cttctcctcg gggcagatct tcaatatagg
     5641 gatcgataat gacttcgaga attcgacgaa tacgaacaac aacaatatcg agcgcgtgga
     5701 ccatattata ggtggcgggg tcagcgcgac ggacggtacg aaaacgggtt ttgtcgtttt
     5761 tgaccggggc aacggtggag gacatgatcc tttttatatc gcggcgataa agacgctgga
     5821 tgccagcggg aacccctcct cctattataa tgccgtctct gcaggggctt ccaattatgg
     5881 ctccgacgtg agcccggcga tgaactatat cacgctgcga aaaaatcctg ccgacacgcg
     5941 tctgctgatg atgaacaaca gcgcaacgca ataccgggac ggggtgcttc tccggttcac
     6001 cgatcttggt gttcccaatg gggccacgat ctatggctat tccctgtttg gtatggatgt
     6061 caatgtcagt tcggccgcca acctggtgga ttacaccaat gccaccaatt tcccaacggg
     6121 ttcggactat gcgaatggtg gtctggacca ggtggcggtg acgggtcttt gggtgaccaa
     6181 cgcctcttat gttgtgctgg ccgacagggt aacggatctt tccgcaaagg tagtgggcgg
     6241 caaggtgcag ttgaactggg ccctgggtgt tatggacgac ctggggcggc ttgtggtgga
     6301 gcggagtgcc gacggggttc attttaattc cctgttggaa tttagcgccc ctctggcggg
     6361 cacgcagacg gtttgggatg aacagccgtt gtccgggcag gatgattacc gcctgaaact
     6421 ggtcgatcaa cggggcgagg tggttgccta tagtatggtg agccgggttg cggtgagtcc
     6481 ggcttccgcg ggggaggcgt ttaccgtcta tccgaacccc gtagccgggc ggcagctgcg
     6541 tttctcgg
//
LOCUS       DATGFG010000300         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1063438, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000300 DATGFG010000000
VERSION     DATGFG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063438"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="VKQ52_05610"
     CDS             complement(<1..1677)
                     /locus_tag="VKQ52_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ86690.1"
                     /translation="MSNCLPNNVFFSSGARGLVLCLLLAMWGNGAWAQQVVKGRVTSG
                     DSAVTNVTVQVKGTTRATQTDLNGQFSLEAAADATLIFSRVGFATQELPVGGRRYFDV
                     HLLIASKEMNEVIVVGYGTQKKADVTGSVASVPKSRLSELPVTNVLQAVEGSVAGVSV
                     TNTSAVPGATPTVLIRGQNSITANSGPLIVVDGIPLSKSGGSINDINPNDIASMEILK
                     DASAVAIYGTNGSNGVILITTKRGATGKPVIRYNGYEGFENLAHILKPRDAKSYVQKY
                     ADYMQQTGQTLTSPVPNASELVNYNLGKTTDWVKETTQQGIIQDHNLSISGGTKDVRY
                     FISGEYLKQKGSIKGYQYHRASFRANLDINVTDFLTIGTSAFVSNNDYGGGRANLLFA
                     TAMSPYGQEFNPDGTYNIYPMYPELLYTNPLIGLYSSNVNHNFNLNGNGYAEVKFGGV
                     LKGLRYRLNTGYNYIPAAVYTYTGRNDNNPLGTATTFNSETNSYTIDNLLLYAREFGK
                     HHVDFTGLYSAEQRRYFVDAYSTTGFINDLLTFNNIGAGATVSAASTGSGL"
     gene            complement(1756..>2811)
                     /locus_tag="VKQ52_05615"
     CDS             complement(1756..>2811)
                     /locus_tag="VKQ52_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ86691.1"
                     /translation="AESGPGSAGGLAGRMAMEGAPVGELSGSGLSVGGLSPLVREMVD
                     EPGKPVSEALSAGGLLMMEPGLGEGELISDRSRVLIVDDDVQILAYMRHVLEGQYIVY
                     QAHSGEEGLELALRHRPELIISDVHMDGISGIELCETVKNDPLLGRTPVILLTASASA
                     NNKLAGVMHGADDYITKPFDKDLLVARVGALLKSRDRMERAFFHVVTNGGTESVRISG
                     EDKEFLDRLIGVVEENLDEDGFCIKKLSVEMGMSHSHLYQKVKELSGQSLNGFIRYIR
                     LKKAAELLISTPCNVNEAAVQVGISDGKYFREQFHKLFGLNPSEYIKKYRKAFSGRYH
                     LNKGEFEGEGGPFGRKL"
BASE COUNT          639 a         1004 c          588 g          580 t
ORIGIN      
        1 cagcccactc cccgtcgaag cggcgctcac cgtggccccc gccccgatat tgttaaaggt
       61 cagcaggtca ttgataaagc ccgtcgtgga atacgcatcc acaaagtatc gccgctgctc
      121 ggcgctgtac aaacccgtaa agtcgacatg atgcttcccg aattcccggg catacaaaag
      181 caggttatcg atcgtataac tattggtctc cgaattgaag gtcgtcgccg tccccaacgg
      241 gttattgtca ttcctgcccg tatacgtata caccgccgca gggatataat tgtaccccgt
      301 attcagccgg tacctcaacc ccttcaacac cccgccgaac ttcacctcgg catagccgtt
      361 cccgttcagg ttgaaattgt ggttcacatt gctggaatag agcccgatca gcggattggt
      421 atacaacagc tcgggataca tgggatagat attgtaggtt ccgtcgggat tgaactcctg
      481 cccatacgga ctcatcgccg tggcgaataa aagattggcg cgtccgccgc cataatcgtt
      541 attgctgaca aaggccgaag tcccgatcgt caggaaatcg gtaacattga tatcgagatt
      601 ggcacggaaa ctcgcacggt gatactggta ccccttgatc gacccctttt gcttcaggta
      661 ctccccggag ataaaatacc ggacatcctt cgttcccccc gaaatagaca ggttatggtc
      721 ctggatgatc ccctgctgcg tcgtctcctt gacccagtcc gtcgtcttcc caagattata
      781 attgaccaat tcgctcgcat tgggcaccgg actcgtcagg gtctgacccg tctgctgcat
      841 atagtccgca tatttctgca cataggactt cgcatcccgg ggcttcagga tatgggcaag
      901 gttctcaaaa ccctcatacc cgttgtaacg gatcaccggc ttacccgtcg ccccccgctt
      961 ggtggtgata agaatgacgc cattggaccc gttcgtccca tagatcgcca ccgcggaggc
     1021 atccttcagg atctccatcg aagcgatatc attgggattg atatcattga tactcccacc
     1081 cgacttggat aaaggaattc cgtccaccac gatcagcgga ccgctattag ccgtgatcga
     1141 attctgcccc cggatgagca ccgtcggcgt cgcacccggc acggccgacg tattcgtcac
     1201 gctcaccccc gcaaccgacc cctccaccgc ctgcaacaca ttcgtcaccg gcagctcgga
     1261 cagccgcgac ttgggcaccg acgccaccga gccggtgaca tccgccttct tctgggttcc
     1321 gtaaccgacc acgatcacct cgttcatctc cttgctcgcg atcagcaggt gaacatcgaa
     1381 ataccgcctt ccccctacgg gcaattcctg ggtggcaaaa cccacccggc taaagatcag
     1441 ggtggcatcc gcggcagcct ccagcgaaaa ctgcccgttc aggtccgtct gggtggcccg
     1501 ggtggttccc ttcacctgga ccgtcacatt ggtcacggcc gaatcgcccg atgtgacccg
     1561 gcccttcacc acctgctgcg cccaggcccc gtttccccac atcgccagca gcaggcaaag
     1621 caccagcccc ctcgcacccg acgaaaagaa tacattgttt ggcaagcaat tactcataaa
     1681 tagttatttg ctgccaaaat accccaaccc cgcgtctacc ataagggggg caatctcaaa
     1741 aaaagggggg taaactcaca atttccgccc aaacggccca ccctcaccct caaactcccc
     1801 cttattcaaa tgatacctcc ccgaaaaagc cttccgatac ttttttatat actccgacgg
     1861 attcaaccca aacaacttat gaaactgctc ccggaaatac ttcccatcac tgatccctac
     1921 ctgcaccgcc gcctcattca cattacacgg cgtactgatc aacaactccg ccgccttctt
     1981 cagccggatg tagcggataa acccattcaa cgactgcccc gacaactcct tcaccttctg
     2041 atacaggtgc gaatgactca tccccatctc cacggaaagc ttctttatac aaaaaccgtc
     2101 ctcatcgaga ttctcctcca ccaccccgat cagccggtcc aggaactcct tgtcctcccc
     2161 gctgatccgg acagactccg tacccccatt cgtcaccaca tggaagaacg cccgctccat
     2221 cctgtcccgg ctcttcaaaa gcgcccccac ccgcgccacc agcaggtctt tatcaaacgg
     2281 ctttgtgata taatcatccg ccccatgcat cacccccgct aatttattat tggccgacgc
     2341 cgacgcggtc agcaggatca ccggcgtccg ccccagcaac gggtcattct tcaccgtctc
     2401 gcaaagctcg atccccgaga tcccatccat atgcacatcg gaaataatca gctcaggccg
     2461 gtgccgcagc gccagctcca acccctcctc cccgctatgc gcctggtaaa caatatattg
     2521 cccctccagc acatgccgca tataggctaa tatctggaca tcatcatcca cgatcagcac
     2581 ccggctcctg tcactgatca actccccctc acccaaaccc ggctccatca tcagcaaacc
     2641 tcccgcactc aatgcctccg acacgggctt ccccggctca tccaccattt ccctcaccaa
     2701 cggcgacaac ccgccaaccg acaacccgct acccgacaac tcgccaaccg gcgccccctc
     2761 catcgccatc cgccccgcca ggcctcccgc cgaccccggc cccgactccg c
//
LOCUS       DATGFG010000301         7148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1532190, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000301 DATGFG010000000
VERSION     DATGFG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532190"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1385
                     /locus_tag="VKQ52_05620"
     CDS             144..1385
                     /locus_tag="VKQ52_05620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ86692.1"
                     /translation="MEKKSPSLFNLAVIVGALGYFVDVYDLLLFSIIRIPSLKGLGLS
                     AADIDSKGGDILSIQMLGLLIGGIIWGVLGDKRGRLKVLFASIILYSLGSIANGFVHT
                     LGQYEAARFITGLGLAGELGAGITLVSELMPKERRGIATSLVAGIGLSGAVFAYFIKA
                     RFGMGADGWRTCYFIGGGLGFLLLLLRVGVLESGMYKSIEDSKVRRGSFLALFTNGQR
                     LKKYLTAILIALPNWYVIGVLITFSNKFGAIMKVTGPIDPGFGIMVAYAAISVGDIAI
                     GFISQGFKSRKKSLWLFHAITIVSVIAFFNMQGQSPSAVYWVCVFLGLGTGFWAMFVT
                     MAAEQFGTNIRATVATTVPNMARGSLTVVVMPLFKALQPSQGYFNAGWITGIIVFAVA
                     SVALALTPETFGKELNYIEPL"
     gene            1386..2390
                     /locus_tag="VKQ52_05625"
     CDS             1386..2390
                     /locus_tag="VKQ52_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLZ86693.1"
                     /translation="MKFLIIRFSSIGDIVLTTPVVRCLKKQVATAEVHYLTKAAFRPI
                     LAANPFVDKIHCLDNNLDATIETLKAEDFDYVIDLHHNLRTLRVKRALKKQAFSFDKL
                     NIQKWLYTNFKINRLPPVHIVDRYLDTLKTFGIKNDGAGLDYFIPKEDELKPGDIPTS
                     HQAGYAGLVIGAALATKRLPFHKLEAICREIRHPLILLGGPEDAETAKKLAAIDPVKV
                     YNACGLFTLNESAGLVRQAKVIITHDTGLMHIAAAFKRPVISIWGNTVPEFGMYPYYG
                     ENFLFHYQGGLLGDHPDLTLEVKGLSCRPCSKIGYDKCPRGHFKCMEQQAIDRIFQYF
                     "
     gene            2400..3458
                     /locus_tag="VKQ52_05630"
     CDS             2400..3458
                     /locus_tag="VKQ52_05630"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HLZ86694.1"
                     /translation="MSGHHLRHDKTCLNCGHLVEERFCTHCGQENLEPKESVGHIVGH
                     FFADITHFDSKLFTTLKDLILRPGFLTREYVAGRRMSYLNPIRMYVFISAVFFLALFA
                     GKKEHEPEKENDQAAVNGYRQHLADSLRAAAFDSARGPFNREMAARLDTVKKEKSKDE
                     SLALNMGTGNVIIDMTEYKYNSVREFDSVQSTLPDSAKDKGLMHWILRHNIRQKEKRG
                     TAKIHLEIDVSHDIPKLMFILLPLFALYVGWFYSRKKYYYVNHAIFSVHFHSFVFLLL
                     MFFLLLGKIIPGDWTEIVLALLSVILTFIYLIAALHGMYGQSFWRTLFKGIGITLLYF
                     ITISIASMLLMVGTYAFL"
     gene            complement(3515..4444)
                     /gene="mdh"
                     /locus_tag="VKQ52_05635"
     CDS             complement(3515..4444)
                     /gene="mdh"
                     /locus_tag="VKQ52_05635"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HLZ86695.1"
                     /translation="MKVTVVGAGAVGATCADNIARAALAEELVLLDIKEGLAEGKAQD
                     MMQTAALLGFDTRITGSTNDYSRTAGSDVVVITSGLPRKPGMTREELIGTNAGIVKGV
                     TENILKHSPNAILIIISNPMDTMTYLALTSTGLPKNRIIGMGGTLDSARFKYQLSQHL
                     GCSPADLNAVVVGGHGDTTMIPLIRLATWNSAPVTKFLSGEQQKQIVADTMVGGATLT
                     KLIGTSAWYAPGAAGAALVEAILRDEKKLFTCCVSLNGEYGQKDICLGVPVVIGRNGW
                     EKILDFGLNAEEQALFNKSAEAVRSMNDVLKTL"
     gene            complement(4477..5013)
                     /locus_tag="VKQ52_05640"
     CDS             complement(4477..5013)
                     /locus_tag="VKQ52_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86696.1"
                     /translation="MKKAFLALMAIACCHFLHAQPTQPNQPATDPANAQAPFLRFPTL
                     PPFHLLKLDSSTYLTKDDVRKNRRTIVMYFSPDCEHCKHEIESFLADWDKFKDIEIVL
                     ATYQPFGEMKEFNTHYRIYEHPNILMGRDEKFFLPPFYKIHNLPYLALYDKKGNLITT
                     FEGTQKVDTLMNAFKEKK"
     gene            5173..5535
                     /locus_tag="VKQ52_05645"
     CDS             5173..5535
                     /locus_tag="VKQ52_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HLZ86697.1"
                     /translation="MEKLSRQEEEAMQAVWKTGEGNVKLFLENMPSGAMPYTTLASTI
                     KNLEKKGFLSSRLIGNAYLYKPVVSEDEYKKRFMNGFVKEYFADSYKELVNFFVEEKK
                     LSAKELREIIELIEKPGR"
     gene            5548..7044
                     /locus_tag="VKQ52_05650"
     CDS             5548..7044
                     /locus_tag="VKQ52_05650"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HLZ86698.1"
                     /translation="MPALFLYLLKLSAAVSVTWLFYYGVLRPLTFYNWNRWYLLGYTL
                     LSFLIPLIDIGPMVTAGDPGQQPRYLSYIPAMGGYSRAVLPTARQSHAWDPWNFALFL
                     LAAGMILLLIRVLIRWYSLTRVRKQARLISSHPDIRIYQVDRHITPFSFGNAVYINQR
                     LHTEQEWEEIILHEYVHIRQRHTIDILLAELISILNWYNPFVWAIRYSIRQNLEFIAD
                     RKVLDNGVDKKGYQYHLLKVVGHQQYRIANNFNFSSLKKRIVMMNKLRSARLHLLKLL
                     FLLPLMAGILLAFRSNYPYLSHGKRDKVYMNKAGDTTIRRRVLTGIRQPKHAAMPTFR
                     DTTPPPVRPALKIGLLEQLDSSHAKSDYRKALFIVDGVERSSRTPDSTLKPADIASMD
                     VLNPDEAVRLFGEKGKYGVVAVLTKAYVNGWGIPSGAKPFLPRKDPVYFVDGVRMPDS
                     VTLATMINPDSIQSIQVLKDQAATALYGPEAGARGVIMIISKSAQKKP"
BASE COUNT         1634 a         2094 c         1780 g         1640 t
ORIGIN      
        1 ttggcttcga tctgaaaaaa cacctggagg tgatcgtcat cggcatcgtc gttatcacca
       61 ccgcccccgt cctgtataaa ttgttcttcg gcaaaaagag ttccgctccc gtcaaagatc
      121 cctaccaaaa atcataattc ctcatggaga aaaaatcacc ttctcttttc aacctggcag
      181 tcattgtcgg cgcgctgggc tattttgtag atgtctacga tctgctcctg ttcagcatca
      241 tccgcatacc ctccctgaaa ggactcggtc tcagcgccgc cgatatcgac agcaagggcg
      301 gggacatcct cagtatccag atgctggggc tgctcatcgg cggcatcatc tggggcgttc
      361 tcggtgataa gcgcggccgg ctgaaagtgc tgttcgcttc gatcatcctc tattcccttg
      421 gcagtatcgc caatggattt gttcataccc tcggccaata cgaggcggca cggtttatca
      481 ccggcctcgg tctggcaggg gaattaggcg cgggcatcac cctggtgagc gaactgatgc
      541 caaaagagcg ccggggtatc gccacctctc tcgtggcggg catcgggctc agcggcgccg
      601 tattcgccta ttttataaag gcccggttcg ggatgggcgc cgatggctgg cgaacctgct
      661 atttcatcgg cggcggcctc ggcttcctcc tcctgctgct ccgcgtcggc gtgctcgaat
      721 cgggcatgta caagagcatc gaggatagca aggtccgcag gggaagcttc ctggcccttt
      781 tcaccaatgg tcagcggctg aaaaaatacc tcaccgccat ccttatcgcc ctgcccaact
      841 ggtatgtcat cggcgtcctg atcaccttct cgaataagtt tggcgcgatc atgaaggtca
      901 cgggacccat cgaccccggc tttggcatca tggtggccta tgccgccatt tcggtggggg
      961 atatcgccat cggattcatc agccagggat tcaaaagccg taaaaaatcc ctctggctct
     1021 ttcacgccat tacgatcgtt tccgttatcg ccttcttcaa tatgcaggga caatcgcctt
     1081 cggccgtata ctgggtgtgc gtattcctgg ggctgggcac gggcttctgg gccatgttcg
     1141 tgacgatggc cgccgaacaa ttcgggacca atatccgcgc taccgtcgcg accaccgttc
     1201 ccaatatggc gcgcggctcc ctcaccgtgg tcgtgatgcc gctgttcaag gccctgcagc
     1261 ccagccaggg ttatttcaac gcgggctgga tcaccgggat cattgttttc gccgtcgcct
     1321 ccgtcgcgct ggcattgacg ccggaaacct ttggaaaaga attgaattat atcgagccac
     1381 tatagatgaa gtttttgatc atccgtttca gctctatcgg cgacatcgta ctcacgacgc
     1441 cggtggtccg ctgtctgaag aagcaggtcg cgacagccga agtgcactat ctcaccaagg
     1501 ccgccttccg gcccatcctg gcggcgaatc ccttcgtcga caagatccat tgcctcgata
     1561 acaacctcga cgcgacgatc gaaacgctga aagcggagga cttcgattac gtcatcgatc
     1621 tgcaccacaa cctccggacg ctgagagtaa aaagagccct gaaaaaacaa gccttctcct
     1681 tcgacaaact caacatccaa aaatggctct acaccaactt taagataaac aggctgccgc
     1741 cggtgcatat cgtcgaccgc tacctggata cgctcaaaac ctttggcata aagaatgacg
     1801 gcgccggttt ggactatttc atcccaaaag aggatgagct aaagcccggg gatatcccca
     1861 cttcccatca ggccggctat gccggactcg tcatcggggc ggcgctggcc acaaaacggc
     1921 tgccgtttca taagctggaa gccatttgcc gggagattcg tcaccccctg atcctgctgg
     1981 gcggacccga ggatgccgaa acggccaaaa aactcgccgc catcgatccg gtaaaggtct
     2041 acaacgcctg cggtctgttt acgctcaatg agtcggccgg gctcgtgcgc caggccaagg
     2101 tgatcatcac ccacgacacc gggctgatgc atatcgcggc ggccttcaag cgccccgtga
     2161 tctccatctg gggcaacacc gtcccggaat tcgggatgta cccgtactat ggcgaaaatt
     2221 ttttatttca ttaccagggc ggtctcctcg gtgaccaccc cgacctcacg ctcgaagtaa
     2281 aaggcctctc ctgccgcccc tgttccaaga tcggctatga caaatgcccc aggggccatt
     2341 ttaaatgcat ggaacaacaa gcgatagatc ggatttttca atatttttag aaaaaaacca
     2401 tgagtggtca ccatctccgc catgacaaga cctgcctgaa ctgcggccac ctcgtggaag
     2461 aaaggttttg tacccactgc ggccaggaga acctcgaacc aaaggaaagt gtcggccata
     2521 tcgtcggcca tttcttcgcc gatatcaccc acttcgactc caaactcttc actacgttaa
     2581 aggatctgat cctccgcccc ggttttctca cccgggaata tgtcgccggc cggcgaatga
     2641 gctatctcaa cccgatccgg atgtatgttt tcatctcggc tgtgttcttc ctggcccttt
     2701 ttgccggtaa gaaagagcac gagccggaaa aggagaacga ccaggcggcc gtcaacggct
     2761 atcgccagca cctcgcggac agcctccgtg ccgctgcctt cgatagcgcc cgggggccct
     2821 ttaatcgcga aatggcggcc cgtctcgata cagtaaaaaa agaaaagtca aaagacgaat
     2881 cactggcatt gaacatgggc accggcaatg tgatcatcga catgacggaa tacaaataca
     2941 acagcgttcg cgagttcgat tccgtccagt caacccttcc cgacagcgcg aaggacaaag
     3001 gcctcatgca ttggatcctg cggcataata tccggcaaaa ggaaaagcgc ggcaccgcaa
     3061 agatccatct cgagatcgat gtcagtcacg atatccccaa actcatgttc attctcttac
     3121 ccttgtttgc cctttatgtc ggatggtttt acagccggaa gaagtattat tatgtcaacc
     3181 atgctatttt ctccgttcac tttcattcct tcgttttcct gctgttaatg ttcttcctcc
     3241 ttctcggtaa gatcatcccc ggagactgga ccgaaattgt ccttgccctg ctgtcagtga
     3301 tcctgacctt tatttatttg atcgccgccc tgcacgggat gtatggacaa tccttctggc
     3361 gtaccctctt taaggggatc gggatcaccc ttctctactt catcaccatc agcatcgcct
     3421 ccatgctgct gatggtcgga acctatgcct ttctttaata ccggcaaaaa aaagccgccc
     3481 tggggggcgg cttcaaaaat ataacaaaaa cgatctacaa cgtcttcaac acatcgttca
     3541 tgctgcgtac ggcttcggcg ctcttgttga acagcgcctg ttcttccgca ttcagcccga
     3601 agtccaggat cttctcccat ccattgcggc cgataacaac gggaaccccg agacagatat
     3661 ccttctgacc atattcgccg ttcagcgaca cacagcaggt gaacagcttc ttctcatcac
     3721 gcaggatcgc ctccaccagc gcggcgccgg cagccccggg ggcataccag gccgatgtac
     3781 cgatcagttt ggtcagggta gccccgccaa ccatggtgtc cgcaacaatc tgcttctgtt
     3841 gctccccact caggaatttg gtcacgggcg cgctgttcca ggtagccagc cggatcagcg
     3901 ggatcatcgt cgtatcccca tgaccaccga caaccacggc attcagatcg gccgggctgc
     3961 aacccagatg ctgcgaaagc tggtatttga accgcgcact gtccagcgtg ccgcccatcc
     4021 cgatgatccg gttcttgggc agaccggtag aagtcagcgc gagataggtc attgtatcca
     4081 tcgggttgct gatgatgatc aggatggcgt tgggcgaatg cttcaggata ttctcggtta
     4141 ctccttttac gatcccggcg ttggtgccga tcagctcttc acgggtcatc cccggtttgc
     4201 ggggaagacc ggaggtgatc accaccacgt cactacccgc cgtcctggaa taatcattcg
     4261 tgctcccggt gatgcgggtg tcaaaaccca gcaacgccgc cgtctgcatc atgtcctgcg
     4321 cctttccttc ggctaatcct tccttaatat ccagcagcac caattcttct gccaatgctg
     4381 cacgggcgat attatcggca caggtagcgc ctacagcgcc ggcgcctaca accgtaactt
     4441 tcatctgtta aaatatttga gtgataaaat ggatgactac ttcttttcct taaaggcatt
     4501 catgagggta tccacttttt gcgttccctc gaaagtggtg atcaggttgc ccttcttgtc
     4561 atacagggca agataaggca ggttgtggat cttgtaaaaa ggcggcagga agaatttctc
     4621 gtcccggccc atcaggatgt tcggatgttc gtagatgcgg taatgcgtat tgaactcctt
     4681 catctcgcca aacggctgat aggtggccag cacgatctcg atgtccttga atttatccca
     4741 gtcggcaaga aaggactcta tctcatgctt gcaatgctcg caatccgggc tgaagtacat
     4801 gacgatcgtc ctgcggttct tcctgacatc gtccttggtc aggtaagtgc tgctgtcgag
     4861 tttcaacagg tgaaagggcg gaagggtagg gaaccggagg aaaggcgcct gggcattggc
     4921 cggatcggtc gccggctgat tgggttgggt aggctgagcg tgcaggaaat gacaacaggc
     4981 gatggccatc agggccagaa atgctttttt catcttcttg aatagtttac aagggaaatc
     5041 cgctgcaaaa taaccggact ccgccgattc agctgcaaaa gtaaccggac tccgccgatt
     5101 gaaacgaaaa agaagatttt ttatttggga attacgaaaa tgtcgtagat ttacgaaaat
     5161 ctcgtaatga ttatggaaaa actcagtcga caggaggagg aagccatgca ggccgtttgg
     5221 aagacgggcg aaggcaacgt caagctcttc ctcgagaaca tgccctcggg ggccatgccg
     5281 tacacgaccc tggcctccac gatcaaaaac ctcgaaaaaa aaggtttcct gtccagccgc
     5341 ctcatcggca atgcgtacct gtacaaaccc gtcgtctcgg aagatgaata caaaaagcgg
     5401 ttcatgaacg gttttgtaaa agaatatttc gccgattcct ataaggagct ggtcaatttc
     5461 tttgtcgaag aaaaaaagct aagcgcaaag gaattgaggg agatcatcga actcatcgaa
     5521 aagccgggtc ggtaaaatca aatcattatg cccgcattat tcctttacct gctcaaacta
     5581 tccgccgctg tttcagtgac ctggctgttt tattacggcg tcctgcgtcc acttaccttc
     5641 tacaactgga accgctggta cctcctgggc tataccctgc tgtccttcct cattcccctg
     5701 atcgatatcg gtcccatggt aacggccggc gacccgggcc aacagccgcg gtacctctcc
     5761 tacattcctg ccatgggcgg ctattcccgg gcagtccttc caaccgccag gcaaagccac
     5821 gcatgggacc cctggaactt cgctctgttc ctgctcgccg caggcatgat cctgttgctt
     5881 atccgcgtac tcatccgctg gtattctctt acccgggtcc ggaagcaggc gcggctcatc
     5941 agcagccatc cggatatccg catctaccag gtggatcggc acatcacccc gttttcgttt
     6001 gggaatgcgg tttacatcaa tcagcggctg cacaccgaac aggaatggga agagatcatc
     6061 ctgcatgaat acgtccatat ccgccagcgg cacaccattg atatcctgct ggctgaattg
     6121 atcagcatcc tcaactggta taatcctttt gtctgggcca tccggtattc catccgccag
     6181 aacctcgaat tcatcgccga ccggaaagtc ctcgacaacg gtgtcgataa aaaggggtat
     6241 cagtatcacc tgctgaaggt ggtaggccat cagcaatacc gcatcgccaa taacttcaat
     6301 ttctcatcct tgaaaaaacg catcgtcatg atgaacaaat taaggagcgc ccgcctgcac
     6361 ttgctcaaat tgctcttcct gctgcccctg atggcaggga tcctcctcgc cttccgcagc
     6421 aactatccat atctgtctca tggaaagcgc gataaggttt acatgaataa agccggcgac
     6481 acaaccattc gccgtcgggt gctcaccggg atccggcagc cgaaacatgc tgccatgccg
     6541 accttcaggg atactacccc gccgcccgtt cgccccgctt tgaagatcgg ccttcttgaa
     6601 cagctggata gctctcatgc aaagtcggat tacaggaaag ccctttttat cgtggatgga
     6661 gtggaaagat cctcccggac acccgactcc accctcaaac cggcggatat cgccagtatg
     6721 gacgtcctga accccgacga ggcggtccgg ctattcgggg aaaaaggcaa atatggggtc
     6781 gtagccgtcc tgacaaaagc ctatgtcaac ggctggggca tcccctccgg cgccaagccc
     6841 ttcctgcccc ggaaagaccc ggtgtacttc gtcgacggcg tacgcatgcc cgacagcgta
     6901 accctggcga caatgatcaa ccccgacagc atccaatcga tccaggtcct gaaggaccag
     6961 gccgccaccg ccctgtacgg cccggaggcc ggcgcccgcg gagtgatcat gatcatcagc
     7021 aaatcagcgc aaaagaagcc ctgacccaat ttttatcgtt ttcggccgaa gtttgccacg
     7081 aagggatttt gatgtaaatt tgccccctcc ttacgggagg acggttctgc tatgtttttt
     7141 agcgatcg
//
LOCUS       DATGFG010000302         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_533963, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000302 DATGFG010000000
VERSION     DATGFG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533963"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1118
                     /locus_tag="VKQ52_05655"
     CDS             75..1118
                     /locus_tag="VKQ52_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86699.1"
                     /translation="MIRYFIRKSFYGLLVLLGVVVTVFFLFQGFGDPSRLVMGQRADA
                     ATQENIRKELYLDQPKWKQFLFYLDDISPMGIYSREEIRQKGLKGVFIGGETVVGLKW
                     PYLRRSYQTKREVGAILMEALPGTLLLALAAMVLATVLGIGLGVLAAIRKDTFWDTSS
                     IFVSVLGISAPSFFMGIVIAYLFGFVLSDVTGLHMTGSLFDTDPFAGRQLQLRNLVLP
                     ALTLGIRPMAIIAQLTRGSLLDVLDQDYIRTAYAKGLKPRVVVWKHALRNALNPVITA
                     ITGWFAELLAGAFFVETIFGWKGIGKVTVDALEKLDFPVVMGSVLVTSFFFILIGVLS
                     DLLYGLVDPRVRL"
     gene            complement(1129..1410)
                     /locus_tag="VKQ52_05660"
     CDS             complement(1129..1410)
                     /locus_tag="VKQ52_05660"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HLZ86700.1"
                     /translation="MGEYSLGDALRKFLNQSKLKGSIQALQIEEVWEQIMGKTVARYT
                     DKIQIHGHTLYVNTAIAPLRQELLYQKDKILQRVNEALGENTIKEVIIK"
     gene            complement(1392..1838)
                     /locus_tag="VKQ52_05665"
     CDS             complement(1392..1838)
                     /locus_tag="VKQ52_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86701.1"
                     /translation="MPSDLESSIRFLEETNDPETAFFSYTVDVTGEYGLIKANKEGLR
                     LYAAEILKKSQQLEEEQRREADPLLFTPRQWMFSETGYDLIAGVLPQYQPRREILTGQ
                     MPPRRGTAVRRRQPVLLFILMCITGALIMLATLKAFPNLRLWVNIR"
     gene            complement(1923..>2296)
                     /locus_tag="VKQ52_05670"
     CDS             complement(1923..>2296)
                     /locus_tag="VKQ52_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86702.1"
                     /translation="LEKDLYLQRTGSGIHKDDIDIRYADEPFKSTASQGQRKSLLFAL
                     KLAEYETLKQSRGWAPILLLDDVFEKLDRHRMRNLLERVCLRDDGQLFITDTHAERIR
                     REMEDLGVAYQLITLPTEGTT"
BASE COUNT          418 a          550 c          747 g          581 t
ORIGIN      
        1 acgatcctgg ggaagtggag ctatgcggat ctcgaccggg ctattccggc cgtgaatgaa
       61 ttgcgcgggc aaaaatgata cgctatttca tacgaaaatc tttttatggg ctactggtgc
      121 tgctgggggt agtggtcacc gtgttcttcc ttttccaggg gtttggcgac ccttcccggc
      181 tggtgatggg gcagcgtgcc gacgcggcca cgcaggagaa tatccgcaag gagctgtacc
      241 tcgatcagcc gaagtggaag caattcctgt tctatctcga tgatatttcc ccgatgggta
      301 tctactcgcg ggaggagatc aggcagaagg gcctgaaggg cgtctttatc ggcggggaga
      361 cggtggtagg gctgaaatgg ccctatctgc ggcggagcta ccagaccaag cgggaggtgg
      421 gggcgatcct gatggaggcc ttgccgggga cgctgctgct tgcgctggcg gcgatggtcc
      481 tggcgacggt gctgggcatc gggctgggag tgctggcggc gatcaggaag gatactttct
      541 gggatacctc ttccatcttt gtgagcgtgc tgggtatctc ggcgccctcc ttctttatgg
      601 ggatcgtcat cgcctatctt tttgggttcg ttctcagcga tgtgacgggc ctgcatatga
      661 ccggtagttt gttcgacacc gatccgtttg cgggcaggca gctgcagtta cgcaacctgg
      721 tattgccggc gctgacgctg gggatcaggc cgatggcgat catcgcccag ctcacccggg
      781 gttcgctgct ggatgtcctc gaccaggatt atatccgcac cgcctatgcc aagggcctga
      841 agccgcgggt ggtggtctgg aagcatgcgt tgcggaatgc gctgaaccct gtgatcaccg
      901 cgatcacggg atggtttgcc gagctgctgg cgggcgcttt ttttgtcgag acgatctttg
      961 gctggaaggg cattggcaag gtgaccgtgg acgccctgga gaagctggat ttcccggtgg
     1021 tgatgggttc ggtgctggtc acttccttct tttttatcct gatcggggtg ttgtccgatc
     1081 tcctgtatgg gctggtggac cccagggttc ggctgtgacg tccgctggct acttgatgat
     1141 cacttctttt atggtattct cgccgagcgc ttcgttgacg cgctggagga tcttgtcttt
     1201 ctggtagagc agctcctggc ggaggggggc gatggccgta ttgacgtaga gggtgtgtcc
     1261 gtggatctgg atcttgtcgg tgtagcgggc gacggtcttg cccatgatct gttcccagac
     1321 ctcttctatc tgcaaggcct ggatggaacc cttcaactta ctttgattca ggaatttccg
     1381 caatgcgtcg cctaacgaat attcacccat aggcgcaagt taggaaaagc ttttagagta
     1441 gccagcatga tgagtgcgcc ggttatgcac ataaggataa aaagcaggac gggctgccga
     1501 cggcgaacgg cggtgccgcg gcggggcggc atctggccgg tgaggatctc gcggcgtggc
     1561 tgatattggg ggaggacgcc ggcgatgagg tcatagccgg tctcactgaa catccattga
     1621 cggggggtga agaggagggg gtcggcttcc cggcgttgtt cttcttcgag ctgttgggat
     1681 ttcttcagga tctccgcggc atagagccgt aatccttcct tgttggcctt tatgaggcca
     1741 tattcgccag taacgtcaac cgtgtaggaa aaaaaggcgg tctcggggtc attcgtttct
     1801 tccaaaaaac gaatagaaga ttccaggtcg gatggcatag caatcaattt tcaggagtgt
     1861 gcaattcggt ggataaatat acattttttt actcagttat ccgacggttc gcgtaaaaaa
     1921 aattaagtgg ttccctctgt gggaagagtg atcagttggt aggctacacc gaggtcttcc
     1981 atttcccggc ggatacgttc ggcatgggta tcggtgataa acaactggcc gtcatcccgc
     2041 aggcagaccc gttccagcag gttgcgcatc cggtggcggt cgagtttctc gaacacgtcg
     2101 tccagcagga ggatgggggc ccaacccctg gactgtttta gcgtttcgta ttcagccagt
     2161 ttaagggcga agagcaggct tttgcgttgt ccctgggagg cggtggattt gaatggttcg
     2221 tccgcatagc ggatatcgat atcgtcctta tggatgccgc tgccggttcg ctgcaggtat
     2281 aggtcttttt cgaggc
//
LOCUS       DATGFG010000303         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_945404, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000303 DATGFG010000000
VERSION     DATGFG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945404"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VKQ52_05675"
     CDS             <1..864
                     /locus_tag="VKQ52_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HLZ86703.1"
                     /translation="MHQVRARIAEVCIGEVESDRQPLHVLGRMAKQIANAIFKRVNIF
                     DGGRREEEQTPHLMREQVEFALKEMVRQPKKMIVFDMDYTLLQGSFIQAAAERFGFTR
                     ELRDILAQPKSNYIKTQKIARLLEGRTFAELINVVESIPLIGDAVQVIRELQIRGYVC
                     GIISDSYHAIANHIKNLLGLDFTLGNELEFQKSVATGEVKIPSQLLKDRFSQCEHDHC
                     KANMFQYILHRFGISVADSVAVGDGENDICMVKMAGTGISFNSSTPGLDEVADYVFRG
                     DSLKPVLEVVR"
     gene            962..>2168
                     /gene="dnaK"
                     /locus_tag="VKQ52_05680"
     CDS             962..>2168
                     /gene="dnaK"
                     /locus_tag="VKQ52_05680"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HLZ86704.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIANDEGRRTTPSIVAFLK
                     NGERKVGDPAKRQAITNPVNTIMSVKRFMGRRYDEVTNEITHWSYKVVRGDNNTVRID
                     IDGRLYTPQEISAMILQKMKKTAEDYLGQEVTEAVITVPAYFNDAQRQATKEAGEIAG
                     LTVRRIVNEPTAAALAYGLDKAGKDEKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDKVVMDWLADEFRKDENVDLRKDPMALQRLKEAAEKAKIELSSSSETEIN
                     LPYITAVDGVPKHLVKKLTRARFEQLSDTLFERCLKPCEQALKDAGISAAQIDEVILV
                     GGSTRIPKVQELVEKFFGKKPNKSVNPDEVVAVGAGIQGAVLTGEVKDVLLLDVTPLS
                     LGIETMGGVM"
BASE COUNT          524 a          525 c          670 g          449 t
ORIGIN      
        1 atgcaccagg tgcgggcgcg gatcgcggag gtttgtatcg gggaggtgga gagcgaccgg
       61 cagccgctgc atgtactggg ccggatggcc aaacagatag ccaatgccat tttcaagcgg
      121 gtgaacatct ttgacggcgg gcgccgggaa gaggagcaga cgccgcacct gatgcgggag
      181 caggtggaat tcgccctgaa ggagatggtc cggcagccga agaagatgat cgtcttcgat
      241 atggactata ccctgttgca ggggagtttt atccaggccg cggccgagcg gttcgggttt
      301 acccgggaat taagagatat tctcgcccag cccaagagca attatataaa gacgcagaag
      361 atcgcccgtc tgctggaagg gaggactttt gccgaactca tcaatgtcgt ggaatccatt
      421 ccccttatcg gggatgcggt gcaggtgatc cgggaattgc agatccgggg atatgtctgc
      481 gggatcatca gtgacagcta tcatgccatt gccaatcata tcaagaacct gctcggactt
      541 gacttcaccc tcggcaacga gctggagttc cagaaaagtg tcgccacggg agaggtaaaa
      601 ataccctcgc agctgttgaa agaccggttc agccagtgcg agcatgacca ttgtaaagcc
      661 aatatgttcc agtacatcct ccaccgattt ggcatcagcg tggctgactc cgtggctgtc
      721 ggggatggcg agaacgatat ctgtatggtc aaaatggcgg ggaccggcat ttccttcaat
      781 tcttccaccc ccggcctgga cgaggtggcc gactacgtat tccgtgggga ttccctgaaa
      841 cctgtcttag aggtagttag atagggatac tgccgatgtg gcagggctac ctgccgtggc
      901 ataatgattg tcaatactat ccggatcaag cttataaaac ctttaaaatc atatatacaa
      961 tatgggaaaa atcataggaa ttgacctcgg caccacgaac agttgtgtgg ccgttatgga
     1021 aggaaatgag cccgtagtga tcgccaacga tgaaggtcgc cggactacac cgtcgatcgt
     1081 ggctttcctg aagaacggag agagaaaagt cggggatcct gccaagcgcc aggccattac
     1141 caatccggtg aacaccatta tgtcggtaaa acggttcatg ggccggcgtt atgacgaagt
     1201 gaccaatgag atcacccact ggagctataa agtggtaagg ggtgacaata atacggtgag
     1261 gatcgatatc gatggccggc tgtatacgcc gcaggagata tcggccatga tcctgcagaa
     1321 gatgaagaag acggccgagg attatcttgg ccaggaagta acggaagccg ttatcacggt
     1381 tcccgcctac ttcaacgacg cacagcgcca ggcgaccaag gaagccggtg agattgccgg
     1441 tctgacggtt cgccgtatcg tgaacgaacc cacggctgcc gccctcgcat atggcctgga
     1501 caaggccggt aaggacgaga agatcgctgt attcgacctt ggcggtggta ccttcgatat
     1561 ctcgatcctc gagttggggg atggggtgtt cgaagtaaag tccaccaatg gtgatacgca
     1621 cctgggtggc gatgatttcg acaaagtggt catggactgg ctggccgatg agttcaggaa
     1681 agacgagaat gtagacctcc ggaaggaccc gatggccctg cagcggctga aggaagccgc
     1741 cgagaaggcc aagatcgagt tgtcctcttc cagcgagacg gagatcaacc tgccctatat
     1801 cacggctgtg gacggcgttc ccaagcattt ggtgaagaag ctgacgcgcg ccaggttcga
     1861 gcagttgtcc gataccctgt ttgaaaggtg tctgaagccc tgtgagcagg cgctgaagga
     1921 tgccggtatc tcggcagcgc agatcgacga ggtgatcctg gtgggtggtt ctacgcgtat
     1981 ccccaaagta caggagcttg tcgaaaagtt cttcggcaag aagccaaata aaagtgtgaa
     2041 cccggatgag gtggtggccg tcggcgccgg tatccagggg gccgttctca ccggtgaggt
     2101 gaaggatgtg ctgctgctgg atgttacccc gctttccctc ggtatcgaga ccatgggcgg
     2161 tgtgatga
//
LOCUS       DATGFG010000304         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_31785, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000304 DATGFG010000000
VERSION     DATGFG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31785"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VKQ52_05685"
     CDS             complement(<1..1661)
                     /locus_tag="VKQ52_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86705.1"
                     /translation="MSQTAPMLRNYLKSAIRILVQNKTFSVINILGLTLGLAAYILIS
                     AYVHFENSFDRQHTGSENIYRVESRFYKGSQLVNDWATSTNGYALAMKDNFPEIEAYT
                     RINFNNSERVVRNTGRDIKFREAHVCFADSNFFSFFSYPLLQGDPHTVLKNTNTVVLS
                     QSAAKRYFGTANPMGKFLDISTFAQTLHCMVTGIFADLPRNSSLQLDFLLSWATSPEW
                     QKTTWYLHESYTYLRLKPGTNIHALEAKFPDLAERYKNGPALKELKWAITLVPLTDIH
                     LNTAKQYEIETKGDRSAIRFLSGIALFILLIACINYINLSTARAVQRAKEVGIRKVSG
                     ARRWQLASQFLLESLLLGTIAFLLAVLLIVGILALSLPYTVLFDTGLCTRIMIIFFAC
                     IVTSGLYPALVLAGLKPIAVLKGRYSFSPAGLRFRKGLVIFQFALSLLLIAGTLAVFR
                     QIRFMMRQSLGVDITQTLVVKAPVNTANYDQKTAALRSALVGLPGVKGVTSSGAVPGR
                     EVGEFLANRRFSDAKQNERLYEMLKVDPGFIRLYGMTVLAGHGFE"
     gene            1971..2195
                     /locus_tag="VKQ52_05690"
     CDS             1971..2195
                     /locus_tag="VKQ52_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86706.1"
                     /translation="MKNKQKKPDSGAHYAGTPPIQHSEVILAQRAKPGEKKAEEKSID
                     EINHDDIESDRHNGSGGAFEATEREWDDEE"
     gene            2260..>2666
                     /locus_tag="VKQ52_05695"
     CDS             2260..>2666
                     /locus_tag="VKQ52_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLZ86707.1"
                     /translation="MASVFSKLKQAYHLFKEVDLDQLGRLASKVDLPAVLKTVGKMDE
                     RQLQGLLKMLGGGAHARKELPAIEGDFYHLAHLLTDEQRALQLKVRAFMETEIKPIVN
                     RYWLKGEFPHEIIPRLASLNICGVTYEGYGCPHL"
BASE COUNT          624 a          579 c          868 g          595 t
ORIGIN      
        1 tgttcgaagc catgaccggc gaggaccgtc attccataca accggataaa gccggggtcg
       61 acctttagca tttcatagag ccgttcgttt tgttttgcgt cgctaaagcg tctgttggcg
      121 aggaactccc ctacctctct gccgggaacg gcacccgaac ttgtgacgcc ttttacaccg
      181 gggagaccga ccagggccga tcgtaatgcg gcggtttttt ggtcgtaatt ggccgtgttg
      241 accggggcct ttaccacgag ggtctgggta atgtcaacac cgagcgactg tctcatcata
      301 aaccggatct gccggaatac ggccagggtt ccggcgatga gcagcaggga aagggcaaat
      361 tgaaaaatga ccagtccttt ccggaaccgc agacccgcgg gggaaaagga atagcggcct
      421 tttaatacgg cgatcggttt gaggccggcg agcaccaggg cgggatacag accgctggta
      481 acgatgcagg cgaagaagat gatcatgatc cttgtacaaa ggccggtatc gaataatacc
      541 gtatagggaa gggagagggc gaggataccc acgatgagga ggacggccag gagaaaggcg
      601 atggttccga ggagcaggga ttcgaggagg aattgtgagg ccagttgcca gcggcgggcg
      661 ccgctaacct tgcggatgcc tacttctttt gcgcgctgga cggcgcgggc ggttgacaga
      721 ttgatataat ttatgcaggc gataagcagg atgaacaggg cgataccgga gaggaagcgg
      781 atggcgctgc ggtcgccttt tgtttcgatc tcgtattgtt tggccgtatt gagatggatg
      841 tcggtgagcg gaacgagggt gatggcccat ttgagttcct tcagcgccgg gccgttctta
      901 tatctctccg ccagatcggg gaattttgct tcgagggcat ggatattcgt tcccggcttt
      961 aatcgcaggt aggtgtagct ttcgtgcagg taccaggttg tcttttgcca ttcgggggag
     1021 gtagcccagg agagaaggaa gtcgagctgc aggctggaat tccgggggag atcggcgaag
     1081 atgccggtga ccatacagtg cagggtctgg gcgaaggtgc tgatgtcgag gaatttcccc
     1141 atggggttgg ctgttccgaa atagcgtttg gcggccgact gcgaaaggac gacggtattg
     1201 gtgttcttta gcacggtgtg ggggtcgccc tgtagcaatg ggtaagagaa gaaagaaaag
     1261 aaattgctgt cggcgaagca gacatgggct tcacggaatt ttatatcccg accggtattc
     1321 ctcacgacgc gttcggagtt gttgaagttt atgcgggtgt aggcttctat ttcggggaag
     1381 ttatccttca tggcgagggc atagccattt gtgctggtgg cccagtcgtt gacgagttgg
     1441 ctgcctttat aaaaccggct ttcgacgcgg taaatatttt cgctgccggt gtgctggcga
     1501 tcgaagctgt tctcgaagtg cacgtaggcg ctgatgagga tgtaggcggc gaggccgagg
     1561 gtgagaccga ggatgttgat tacggagaag gttttgttct gaacgaggat gcggatagca
     1621 gacttcaggt aattgcggag catgggggca gtttgggaca aattaccgat tttatcggat
     1681 aaatggccat gccgccgaca agggaccagg agcattttat caaggcgggc taacgcatgt
     1741 atttgctgta accagggacg gatcatcgtt gttggaacaa tgaatagcct ttaataggtg
     1801 ttagcaacgg cgatagataa aagctcccgg tcccaggatc agcggacgtt gcggttctaa
     1861 taatattgta aagggaacac tacggttgga ggggccgggc gaaaatacag aaaaatccgg
     1921 tatggcgggt atccggaggg ggctggcatg gattttttat aggttatgga atgaaaaaca
     1981 agcaaaaaaa accggatagc ggcgcgcatt atgcgggcac gccacccatc cagcattccg
     2041 aagtgatcct ggcgcagcgg gctaaacccg gagagaagaa agcggaggag aagtcgatcg
     2101 acgagatcaa ccatgacgac atcgaatccg atcgtcataa tggttcgggc ggcgccttcg
     2161 aggccacgga gcgggagtgg gatgatgagg aatagtttag cgagggtggg cgcgtcctcg
     2221 gaaaaagtgc tattttgccg gctaacctat ttgtttagca tggcatcggt tttctccaaa
     2281 ctcaaacagg cctatcacct cttcaaggag gtagaccttg accaattggg ccggctggcc
     2341 tcgaaggtag atcttcccgc agtattaaag accgtcggta aaatggatga gcgacagcta
     2401 caggggttgc tcaagatgct tggcggcggc gcgcatgcgc gcaaggaatt gccggcgata
     2461 gaaggagact tttatcatct tgcgcatttg ctgaccgatg agcagcgggc gctgcaactg
     2521 aaagtacgcg catttatgga aaccgagatc aagccgatcg tcaaccggta ttggttgaag
     2581 ggggagtttc cgcatgagat catcccccgg ctggcgtcgc tgaatatctg cggggtcacc
     2641 tatgaagggt atgggtgtcc gcatct
//
LOCUS       DATGFG010000305         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1408619, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000305 DATGFG010000000
VERSION     DATGFG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408619"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VKQ52_05700"
     CDS             complement(<1..1280)
                     /locus_tag="VKQ52_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLZ86708.1"
                     /translation="MNTSLHRRQLLKMVGLGSAATIFGNLTATAASRHAKTDDDPAHT
                     AGELPAATTGKPPLAASTTGTPNRTGLPPVTIRSVKAIGTAPQGANLVVVKVETSEPG
                     LYGLGCATFTQRAEVVVTAINNYMPDFCVGKNVDDIEDLWQSFFVSSYWRNGPVLNNA
                     LSGLNQALWDIKGKRANMPVYQLLGGKARFAVDCYAHADGHTPEEVAASVQKFMDQGF
                     RHVRIQQGGYGSLQLAKQPDWRDAGFGRPTDDFMDTHAYVKAVPKLFEVVRKTCGENV
                     ELLHDTHERVQPQDMINMCRRLEEYDPYWIEDPLSPENMHLFPQLRAATTVPFAMGEL
                     FNNPNEFVDYIARRDFDYFRCHVSQIGGISPAIKVAHLGEFFNVRSGWHGPGDVSPVG
                     HAAQCHIDLAIWNFGVQESVFFNDQTKEVFPGTP"
     gene            complement(1396..>2026)
                     /locus_tag="VKQ52_05705"
     CDS             complement(1396..>2026)
                     /locus_tag="VKQ52_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86709.1"
                     /translation="KLSNSANGYNRTASFVSYHGPKDSTRGSRLLFNNWPRGYVLGAY
                     DTLLKNYNLFLNFDKITRALYFTLDGKTIVKVQTDQARELHFTDSNTQLVLVRVDGID
                     PRLFFQRLSDSSGNDHYILYRLTKTQYRRANYQTDGLVSTGNNYDEYVDSYDYYLVMP
                     GGKLYTPVKLKKKDIRAALGSKADAWLDAHRNSPPDESFLIGLVNHLNN"
BASE COUNT          363 a          442 c          750 g          471 t
ORIGIN      
        1 tcgggggtgc cggggaagac ctcttttgtc tggtcgttga agaagacgga ttcctgtacg
       61 ccgaagttcc agatggcgag gtcgatatgg cactgggcgg catggccgac gggggataca
      121 tcgccggggc cgtgccagcc ggagcggacg ttgaagaatt cgccgaggtg ggcgactttt
      181 atggcggggc tgatgccgcc gatctgggat acgtgacagc ggaaatagtc gaagtcgcgg
      241 cgggctatat aatcgacgaa ctcgttcggg ttgttgaaga gctctcccat ggcaaagggg
      301 acggttgtgg cggcccgaag ttgggggaag aggtgcatgt tctcggggga gagggggtct
      361 tcgatccagt aggggtcgta ttcttcgagg cggcggcaca tattgatcat atcctgtggc
      421 tggacgcgct cgtgcgtgtc gtggaggagt tcgacgtttt cgccgcatgt tttgcggacg
      481 acctcgaaga gtttggggac ggcttttacg taggcatggg tgtccatgaa gtcatcggtg
      541 gggcggccga agccggcgtc gcgccagtcg ggttgtttgg ccaattggag ggagccgtag
      601 ccgccttgtt ggatgcgaac gtggcggaaa ccctgatcca taaatttctg cacgcttgcc
      661 gccacctcct ccggcgtatg gccgtcggcg tgggcgtagc agtcgacggc gaagcgggcc
      721 ttgccgccga ggagctggta gacgggcatg ttggcccgtt tccctttgat atcccagagg
      781 gcctggttga ggccgctgag cgcgttgttg aggacggggc cattgcgcca gtaggagctg
      841 acgaagaagg actgccagag atcttcgatg tcatcgacgt tcttgccgac gcagaagtcg
      901 ggcatatagt tatttatggc cgtgacgacg acttcggcgc gctgggtgaa ggtggcgcag
      961 ccgaggccgt agaggccggg ttcggaggtt tctaccttga ccacgacgag gttggcgccc
     1021 tggggggcag tgccgatggc ttttacagat cggatggtaa ctggggggag gccggtgcgg
     1081 ttgggggtgc cggttgtgga ggcggctagc ggcggttttc cagtcgtggc ggccgggagt
     1141 tccccggctg tgtgagccgg gtcgtcatcc gttttggcgt gccgggatgc agcggtggcg
     1201 gtcagattgc cgaagatggt ggccgcggag ccgagaccga ccattttcag gagttggcgg
     1261 cgatggaggg aagtattcat atggaagttg ttttactgaa ccggaaatgc gtttcttgca
     1321 gctcctggat ggcagcagga tgcaagtaag cctgcggggc ttaacagagt gattaattta
     1381 cgactaatcc tgaaattagt tattaaggtg attaaccaac ccgatgagga aggattcgtc
     1441 cggcggagag ttccggtgtg cgtccagcca ggcgtctgcc tttgagccga gggctgcacg
     1501 gatgtccttt ttctttaatt tgacgggggt gtagagctta ccgccgggca tgacgaggta
     1561 gtagtcgtag gagtcgacat attcatcata gttattgccg gtggagacga ggccgtccgt
     1621 ctggtagttg gcgcggcggt attgggtttt tgtcaggcga tagaggatat agtggtcgtt
     1681 cccactggaa tcgctaaggc gttggaagaa gagcctgggg tcgatgccgt cgacacgtac
     1741 gaggacgagt tgggtattgc tgtcggtgaa gtgaagttcg cgggcctggt cggtctggac
     1801 cttcacgatg gtcttgccgt cgagggtgaa atagagggca cgggtgatct tgtcgaagtt
     1861 gaggaagagg ttgtaatttt taaggagggt gtcgtaggca ccgaggacgt agcctcttgg
     1921 ccagttgttg aagagcaggc ggctgcctct tgtggagtct tttggtccgt ggtaggatac
     1981 aaagctggcg gtgcgattgt acccgttggc gctgttggag agcttg
//
LOCUS       DATGFG010000306         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_676432, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000306 DATGFG010000000
VERSION     DATGFG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676432"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VKQ52_05710"
     CDS             complement(<1..831)
                     /locus_tag="VKQ52_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HLZ86710.1"
                     /translation="MAKSKIAEDFHENESGVTGQYKNWFLDYASYVILERAVPAIEDG
                     LKPVQRRILHAMKEMDDGRFNKVANIIGQSMQYHPHGDASIGEALVNMGQKDLLIDTQ
                     GNWGDVRTGDDAAASRYIEARLSKFALEVAFNAKTTDWQLSYDGRKNEPVTLPMKFPL
                     LLAQGAEGIAVGLATKILPHNFCELCEAAMKYLKGRKFELYPDFLTGGMIDTTNYNDG
                     KRGGKVRVRTHIEELDKKTLLVKDVPYGVTTTQLIDSILKANDQGKIKVRKVTDNTAA
                     E"
     gene            complement(834..1205)
                     /locus_tag="VKQ52_05715"
     CDS             complement(834..1205)
                     /locus_tag="VKQ52_05715"
                     /inference="COORDINATES: protein motif:HMM:NF025445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HLZ86711.1"
                     /translation="MKQLIKWEEAALLLLSIFLWAQLHFAWYWWLIWILAPDLSMIAY
                     LGGNRVGAIGYDLVHHKAVAVVVYLVGWYLGDRIVQGAGMILLGHSCMDRAMGYGLKY
                     FSGFQDTHLGKIGKDRNKTTN"
     gene            complement(1232..2086)
                     /locus_tag="VKQ52_05720"
     CDS             complement(1232..2086)
                     /locus_tag="VKQ52_05720"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HLZ86712.1"
                     /translation="MIHIVFQYNDREVLQKAFELDPSFQGEVISIADEFAVGPIADIY
                     SQEGIEARKQWWREILAGGDHAGLVDNGNLRDDNALVETVKQRLTEDPKEVLWIWAAQ
                     NKHDVSGYYWLMSQLKEFQGRVFILYLNNLPFINEKGQIFYPENLFTIPPREFLKARK
                     LARPITLSEFEIDPDEWNRLCQENKGVRILEGGKKLSQHDYTFYDEELRKFITGDWIK
                     ASRLISQFLSKARQTTGDAYLLWRIKQLVAAGELDVQGELKGMKDFEIRSRAAAAAAV
                     PSVEESAG"
     gene            complement(2091..>2532)
                     /locus_tag="VKQ52_05725"
     CDS             complement(2091..>2532)
                     /locus_tag="VKQ52_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HLZ86713.1"
                     /translation="YNNIVIATDADVDGMHIRLLLMTFFLQFFPDLVKNGHVYILETP
                     LFRVRNKQETHYCYDEAEKQAAVKKLGSKPEITRFKGLGEISPDEFARFIGADMRKQP
                     VMVMQDTHIQQILEYYMGKNTPERQEFIIQNLKVELDLVEEEKA"
BASE COUNT          524 a          848 c          560 g          600 t
ORIGIN      
        1 ctccgcggcc gtattgtccg tcaccttccg cacctttatc ttcccctggt cgttcgcctt
       61 cagaatggaa tcgatcagtt gtgtcgtcgt cacgccgtaa ggcacgtctt ttaccagcag
      121 cgtcttctta tcgagctcct cgatatgtgt ccggacgcgg accttcccgc cccgttttcc
      181 gtcattgtaa ttcgtcgtgt cgatcatccc gcccgtgagg aaatccggat aaagctcgaa
      241 cttcctgccc ttcaggtact tcatcgccgc ctcgcacaat tcacaaaaat tatgcggcag
      301 gatcttggtc gccagcccta cggcaatccc ctccgccccc tgcgccagca gcagcgggaa
      361 tttcatcggc agcgtgacag gctcgttctt ccggccatca taactaagct gccaatccgt
      421 ggtcttggca ttgaaggcta cctccagcgc gaacttgctg agccgcgcct cgatataccg
      481 ggaagcggcc gcgtcatccc cggtccggac atccccccag ttgccctggg tatcgatcaa
      541 caggtccttc tgccccatat tgacaagcgc ctcaccgata ctcgcatccc cgtgcgggtg
      601 atattgcatg ctctgcccga tgatattggc cactttgttg aaacgcccgt catccatctc
      661 cttcatggca tgcaggatcc gccgctgcac aggcttcaac ccgtcctcga tggccggcac
      721 ggcccgctcc aggataacat aggacgcata atcaaggaac cagttcttat actgccccgt
      781 aaccccggac tcgttctcat gaaaatcttc ggctatctta ctcttcgcca taatcaattt
      841 gtcgttttgt ttctgtcttt cccgatcttc cccagatggg tatcttgaaa tccactaaaa
      901 tattttaatc cataccccat cgccctatcc atgcagctat gccccagaag gatcattccc
      961 gccccctgca ctatccggtc gcccagatac cagcccacga gataaacgac gacggccacc
     1021 gccttgtgat ggacgagatc atacccgatg gccccgaccc tgttgccgcc caggtaggca
     1081 atcatcgaaa gatcgggcgc aaggatccag atcagccacc agtaccaggc aaaatgcagc
     1141 tgcgcccaca agaatatgct aagcaacagg agcgcggcct cttcccattt tataagttgt
     1201 ttcattcgtc tcggtttcgc atggtcgcat cctaacccgc cgattcttcc acggagggca
     1261 ctgccgccgc tgccgccgcc ctcgaccgga tctcgaagtc cttcatcccc ttcaattccc
     1321 cctggacatc cagctcgccc gccgccacca gctgcttgat ccgccatagc agataggcat
     1381 cccccgtggt ctgcctggcc ttgctgagga actggctgat cagcctgctg gcctttatcc
     1441 aatcccccgt aataaatttc cgcaactcct cgtcatagaa ggtgtaatcg tgctgggaca
     1501 gcttcttgcc cccttccagg atccgcaccc ccttgttctc ctggcaaagc cggttccact
     1561 catcgggatc gatctcgaac tcactcagcg tgatcggccg cgccagcttc cgcgccttga
     1621 ggaattcccg cggcgggatg gtgaacaggt tctccgggta aaagatctgc cccttctcat
     1681 tgataaaagg cagattattg agataaagga taaaaaccct cccctgaaat tccttcaact
     1741 gcgacatcag ccagtaatac ccgctgacat catgcttgtt ctgcgccgcc cagatccaga
     1801 gcacctcctt cgggtcctcc gtaagccgct gcttcaccgt ctccaccagg gcattatcat
     1861 cccgtaggtt gccgttatcc accagcccgg catgatctcc gccggccagg atctcccgcc
     1921 accactgctt gcgggcttca atgccctcct gcgaatagat gtccgctatc gggcccaccg
     1981 caaactcatc cgcgatggag atcacctccc cctgaaagga agggtccagt tcgaaggcct
     2041 tttgcaatac ctccctgtca ttgtattgaa aaacaatatg gatcatgcta tcatgctttt
     2101 tcctcttcca cgagatcaag ctccactttc agattttgaa taatgaattc ctgccgctcc
     2161 ggcgtattct tccccatata gtattccagt atctgctgga tatgggtatc ctgcatcacc
     2221 atcaccggct gcttgcgcat gtcggcgccg ataaaccgcg caaactcatc cggggagatc
     2281 tcgcccaacc ccttgaaccg tgtgatctcc ggcttggagc ccagcttctt caccgccgcc
     2341 tgcttctccg cctcatcata gcaataatgc gtctcctgct tgttccgcac ccggaacaac
     2401 ggcgtctcca ggatatacac atggccgttc ttaacaagat cggggaagaa ctgcaggaaa
     2461 aaggtcatca gcaacagccg gatatgcatc ccgtccacat ccgcatcggt ggcgatgacg
     2521 atattgttgt ac
//
LOCUS       DATGFG010000307         4987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_869297, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000307 DATGFG010000000
VERSION     DATGFG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869297"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VKQ52_05730"
     CDS             <1..816
                     /locus_tag="VKQ52_05730"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HLZ86714.1"
                     /translation="QTDFAVKTKNNGEFGLKLSAYLDQVKLIRPSEFNTTDTITNTSG
                     GTTTYTTASGKTVTLSSGSTYVTEGTSTIPSRSRDTYTASFSFTQVINTRLQGAILLD
                     LVAQNGYLGLPFHRVYLKDGTDTLENLPSTRFKLPVGLRLNYFMGDRVILRSYYRFYI
                     DDWGVRSHTASLEVPVKISPFFSISPFYRYYVQTASKYFAPYETHTAAETYYTSNYAL
                     AGFSSQFFGAGIRLSPPDGIAHTHLSTLEIRYGHYTQTTDLVSDVISLNLSFK"
     gene            856..2241
                     /locus_tag="VKQ52_05735"
     CDS             856..2241
                     /locus_tag="VKQ52_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ86715.1"
                     /translation="MKSIITTARALLFIAVLCLAGNSGLEAQQTTILTNATIIDGTGR
                     EPQPNMAMIIQGGHITKITRSKIAIPDGAVQVDMTGKYLMPELINCHGHLGNLLDTVA
                     SSDNYTRGNVIRQLKHYEDYGVGAVLSMGTEQPLGIAVRADSRAGLLPGATMYSALFG
                     FGVKGAMPPESAGFTAIYRPGTPEEAARDVQELATWRPDVIKIWVDNYYGQFQTSMRE
                     DIYTAIIAEAHKHGIRVAAHVYHLADAHKLVAAGLDIMAHSIRDAEIDDTLIEEMKAR
                     HVAYIPTLSLDEYATAYLDDPSWLNDPFFRNSLEPGVYAMITSPAYKEKLRKSPVTPQ
                     EKEALRFALINLRKLYKAGILVAMGTDSGATPIRTQGFSEHLELELMVQAGLTPLEAI
                     KVATYNGARLLKCADDRGTLEPGKRASFLVLEKDPTADIRNTRTLTAVWSDGVKVSDG
                     PLAINKATADR"
     gene            2281..3207
                     /locus_tag="VKQ52_05740"
     CDS             2281..3207
                     /locus_tag="VKQ52_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ86716.1"
                     /translation="METDAGSRDSLTWEFDGIRMGHAISRFNTLTSFSASGSATDVVR
                     LHIGLKGNYSFHYRQLDTDYHLIGGHHNILYSKDFDMTVKNRTLTLETFGIQFPRETF
                     LRFTQNASDLLKRFAQHLEAGDSAILSDQWGAVDPGIQQVVHQIINNPYKGDLQQIFL
                     LSKSIELLVLCAESCQLAIEKKEEFVKSPADKEKILAVRDLINEKLDCPPNLSTISRT
                     VGLNEYKLKRGFKEVFNTTVFGYLTEQRLLLARQYLLDTQHTAADIAYQLGYSTPQHF
                     NNAFKKRFGNTPDSIRPEGVRSVRDAKSQKNP"
     gene            3258..3710
                     /locus_tag="VKQ52_05745"
     CDS             3258..3710
                     /locus_tag="VKQ52_05745"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLZ86717.1"
                     /translation="MNTPLIVKSEITINAPATSVWDALTRPEWTKQYMFGCEAQSDWK
                     PGSPLLWRGNFNGVDMVAVKGQIVSIDPVQSLVYTVIDPNNPAIPDIPENYLTVTCQL
                     TEEGGRTFLTVTQGDYSKVAEGETRYQHTVDGGGWDPILVQIRDLLEK"
     gene            3908..>4987
                     /locus_tag="VKQ52_05750"
     CDS             3908..>4987
                     /locus_tag="VKQ52_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86718.1"
                     /translation="MARKLSGEATAEEIAELERFQQQHPEMTYSLQMIADLWRSQNPT
                     DDAEADQAFNRHLTRMALRDIQPAEPAPNTPANPRGPGATAGAAPTNAATSRASFAQT
                     RDLVANYFNTTLRSLRRNKGFTAINISGLAIGLASAIVLLLWIRNELSFDQFHVNRER
                     VYQAMTRRPLDGQLDVFGRTPSVMAPFLKANYSAQIEEAVRINWVGAFIFSNGDKHLQ
                     TQGYLCDSGFFHLFSFPLLRGNPNTALRSPRSVVLTERLSKKLFGSIDPLGRTVSIDS
                     TSNFVVTGVMKDQPPNTLFQFEYLLPWSYTKEVHWEETKWQKATITTFVLLRPGVTEA
                     AANQAMRMVIQSHAREIPAELFFHPM"
BASE COUNT         1131 a         1821 c         1148 g          887 t
ORIGIN      
        1 cagaccgatt tcgccgtcaa aacgaaaaac aacggggaat tcgggctcaa actgtccgcc
       61 tacctcgatc aggtgaaact catccgcccc tccgagttca acaccaccga cacgatcacc
      121 aacaccagcg gcggcaccac cacctatacc actgccagcg gcaagaccgt caccctcagc
      181 tctggcagca cctacgtcac cgaaggcacg agcaccatcc cctcaaggtc ccgggacacc
      241 tacaccgcct ccttctcgtt cacccaggtc atcaacaccc gcctgcaggg cgccatcctc
      301 ctcgacctcg tcgcgcagaa cggctatctc ggcctgccct tccatcgcgt ctacctcaag
      361 gacggcaccg acaccctgga gaatctcccc tccacccgtt tcaaactgcc ggtaggcctg
      421 cgcctcaatt acttcatggg cgatcgggtc attctccgct cctactaccg tttctacatc
      481 gacgactggg gcgtccgctc gcacacggcc agcctcgaag tcccggtaaa gatcagcccc
      541 ttcttctcca tcagcccttt ctaccggtat tatgtacaaa cggcttcgaa atatttcgca
      601 ccctacgaga cgcacacggc cgccgaaacc tattatacca gcaactatgc cctggccggc
      661 ttctcgagcc agttctttgg cgccggcatc cgcctctcgc cgccggacgg catcgcccac
      721 acccatctaa gcaccctcga gatccgttat gggcattata cacagacaac cgatctggta
      781 tccgacgtta tttcgttaaa tctctcgttc aaatgaggtt gacggcgtaa cttgctgttc
      841 gaaaccatac attccatgaa atcgatcatt accaccgcgc gggcgttgct cttcatcgcc
      901 gttctgtgtc tggcgggtaa cagcggtctc gaagcccaac aaactactat tcttaccaat
      961 gccaccatta tcgatggcac cggccgggaa ccgcagccca atatggccat gatcatccag
     1021 gggggccaca tcacgaagat cacccgcagc aagatcgcca tccccgacgg cgctgtacag
     1081 gtggacatga ccggtaaata cctcatgccc gagctcatca actgtcacgg tcacctcggc
     1141 aacctcctcg acaccgttgc ctcttcggac aactataccc gcggaaatgt catccgtcag
     1201 ttgaaacact acgaagatta tggggtaggg gccgtccttt cgatggggac ggaacaaccc
     1261 ctgggcatcg ccgtccgcgc ggactcccgt gcggggctcc tacccggggc aaccatgtac
     1321 tccgccctct ttggctttgg tgtaaaaggc gccatgcccc ccgaatccgc cggcttcacg
     1381 gctatctacc ggcccgggac acccgaagag gcggcccgcg acgtccagga actcgccacc
     1441 tggcgccccg acgtcataaa gatatgggtc gacaactact acggccagtt ccagaccagc
     1501 atgagggaag atatctacac tgccatcatc gccgaggcgc acaaacacgg catccgcgtc
     1561 gccgcccatg tctaccatct ggcggacgcg cataaactgg tcgccgcagg cctcgacatc
     1621 atggcccaca gcatccgcga cgccgagatc gacgatacgc tcatcgaaga aatgaaggcc
     1681 aggcatgttg cctatattcc gacgctctcg ctcgacgaat atgcgacggc ctacctcgac
     1741 gaccccagct ggctgaacga ccccttcttc cgcaactccc tcgaacccgg agtatatgcg
     1801 atgatcacca gcccggcgta caaggaaaag ctgcgcaaga gccccgtcac gccacaggag
     1861 aaggaagcgc tgcgttttgc gcttatcaat cttcgcaaac tctataaggc aggcatcctc
     1921 gtggcgatgg gcaccgactc cggcgcgacc cccatccgca cccagggctt ttcggaacat
     1981 ctcgaactgg aactgatggt ccaggccggt ctcacgccgc tcgaagcgat caaggtcgcg
     2041 acctataacg gcgcccggct cctgaagtgc gccgacgatc gcggcaccct cgaacccggc
     2101 aaacgcgcca gcttcctcgt cctcgaaaag gaccccaccg ccgacatccg caacacccgc
     2161 accctcaccg ccgtctggtc cgatggcgtc aaggtcagcg acggcccgct cgcgatcaac
     2221 aaggcgactg cggaccgcta aacacctatc tttgccctga ccatgtcgac tccccaacat
     2281 atggaaaccg atgccggctc ccgtgactcg ctcacctggg aattcgacgg catccgcatg
     2341 ggccatgcca tctcccggtt caacaccctc accagcttta gcgccagcgg ctccgccacc
     2401 gatgtcgtcc gcctccatat cggcctcaag ggcaactaca gcttccacta tcgccagctc
     2461 gataccgact atcatcttat cggcggtcat cacaacatcc tgtactcaaa ggacttcgac
     2521 atgaccgtaa agaaccgcac cctcacgctg gagacctttg gcatccaatt cccccgcgaa
     2581 acctttctcc gcttcaccca gaatgccagc gatctcctca agcgctttgc ccagcacctg
     2641 gaagccggtg actccgccat cctctccgac caatggggcg ccgtcgaccc cggcatccag
     2701 caggtggtcc accagatcat caacaacccc tacaaaggtg atctccagca gatcttcctc
     2761 ctcagcaaaa gcatcgaact cctcgtcctc tgcgccgaat cctgccagct cgccatcgaa
     2821 aaaaaggaag aattcgtaaa gtcgcctgcc gacaaggaga agatcctcgc cgtccgcgac
     2881 ctcatcaacg aaaaactcga ctgcccgccc aatctctcca ccatctcccg caccgtcggg
     2941 ctcaacgaat acaagctgaa gcggggcttc aaggaggtct tcaacaccac cgtctttggc
     3001 tatctcaccg aacaacgcct cctcctcgcc cgccaatatt tactcgatac ccaacacacc
     3061 gccgccgata tcgcgtatca gctcggctac tccacccccc agcatttcaa caacgccttc
     3121 aagaaacgat tcggcaacac gcccgattcc atccggccgg agggggtccg gtctgtccgc
     3181 gacgcaaaaa gccaaaaaaa tccgtaaaat gcaatcccgg tcgggaccac ccggctacta
     3241 ttgcataaaa aaagcatatg aacactcccc tgattgtaaa aagtgagatc acgatcaacg
     3301 cacccgcaac atccgtctgg gatgcgctta cccggcccga atggacaaaa caatacatgt
     3361 ttggctgcga ggcccagtcc gattggaaac ccggcagccc cctgctctgg agggggaact
     3421 tcaacggcgt tgacatggtg gcggtaaaag ggcagattgt cagtatcgac cctgttcaga
     3481 gcctcgtgta tacggtcatc gaccccaaca accccgccat ccccgacatc ccggagaact
     3541 acctcacggt cacctgccaa ctgaccgaag agggcggccg gaccttcctc accgtcaccc
     3601 agggtgacta cagcaaggtg gccgaaggcg aaacccgcta ccagcatacc gtcgacggcg
     3661 gcggctggga ccccatcctc gtccagatca gggatctcct cgaaaaatag ctcctgcgcc
     3721 cccgccgcac cggccagcct gcctccgcgg cgccggccgc gggggcgccc cttttttccc
     3781 ggcgcatgca acattcctcc cgataattcc gatctttggc cgtaacggtc ccaataaaac
     3841 cgtcgtcaaa taacctgcta aaagcgccac cgctcatatg aaaggacaag accggatatg
     3901 gatcttgatg gctcgcaaac tgtccggcga ggcaacggcc gaagagatcg ccgagctcga
     3961 acgctttcag cagcaacacc ccgaaatgac ctactccctc cagatgatcg ccgatctctg
     4021 gagatcccaa aaccccaccg acgacgccga agccgaccag gccttcaacc gccacctcac
     4081 ccgcatggcc ctccgcgaca tccaacccgc ggaacccgcc cccaacaccc ccgcaaaccc
     4141 acgcggtccc ggcgccaccg ccggcgcggc ccccacaaat gccgccacca gccgcgcctc
     4201 ctttgcccaa acccgcgacc tcgtcgccaa ctacttcaac accaccctcc gcagccttcg
     4261 ccgcaacaag ggcttcaccg ccatcaatat ctccggcctc gccatcggcc tggccagcgc
     4321 catcgtcctc ctcctctgga tccgtaatga gctttccttc gaccagttcc atgtcaaccg
     4381 cgaacgggtg taccaggcga tgacccggcg gcctctcgac ggacagctcg acgtgttcgg
     4441 ccgtaccccc agcgtgatgg cgccttttct aaaagccaac tattccgcgc agatagaaga
     4501 ggccgtccgc atcaactggg tcggcgcctt tatcttctcc aatggggaca aacacctcca
     4561 gacccagggc tatctctgcg actcgggctt ctttcacctc ttctcctttc ccttgctgag
     4621 aggcaatccc aacaccgccc tgcgatcacc ccggagcgtg gtccttaccg aaaggctgtc
     4681 caaaaaactc tttggcagca ttgaccccct cggcaggacc gtcagcatcg acagcaccag
     4741 caacttcgtc gtcaccggcg taatgaagga ccagcccccc aacacgctgt tccagtttga
     4801 atacctcctt ccctggtctt atacaaagga agtccactgg gaagaaacga aatggcaaaa
     4861 agcgaccatc accacctttg tcctgctgcg acccggcgtc accgaagccg ccgccaacca
     4921 ggccatgcgc atggtcatcc agagtcatgc ccgggagatc cccgcggagc tcttcttcca
     4981 tcccatg
//
LOCUS       DATGFG010000308         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_646973, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000308 DATGFG010000000
VERSION     DATGFG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646973"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VKQ52_05755"
     CDS             <1..734
                     /locus_tag="VKQ52_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ86719.1"
                     /translation="EVRYYPLVQLFIVALFIFFTLYSITVRNKATQNQLWAGMAKETA
                     HQLGTPVSSLEGWVEMLRESAVDPRTVQEIQKDVDRLKLVSDRFGKIGSKPVLEEKDL
                     VGQVQTMMEYIRKRATGKVQFVLHEPVTTPLYARISGPLFDWVIENLLKNALDAMEGK
                     GSITIGIVDHEKEIVIDVTDTGKGIAARNLQKVFKPGFTTKKRGWGLGLSLSKRIIEQ
                     YHQGQLFVRQSELGKGTTFRIVLRK"
     gene            743..1597
                     /locus_tag="VKQ52_05760"
     CDS             743..1597
                     /locus_tag="VKQ52_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ86720.1"
                     /translation="MERYFEQNYKKLGVVEVDEAAVAEGIDEQHYKSYIRVLYLPAGY
                     SLTVDFKRYDTKKGTLFFINSNQYFHLDRRGAGMGYLIYYNRDFYCVQIHDEEVACDG
                     LLFNNIYGMPMTVLPPKEERLVSGALQHILEELSHRESAYEEMVRTYLKQMIILATRI
                     WKQQQLGQLKEQPGEDIEFFRTFSRLVEIHFREKHSVNEYAELLGVAAKTLSKKFNRL
                     RLTQPNEVIKDRILLEAKRLLVYSALSIKEIAYDLGYEDPAYFNRLFTAKAGCTPAVF
                     RKGHGGEG"
     gene            1854..2831
                     /locus_tag="VKQ52_05765"
     CDS             1854..2831
                     /locus_tag="VKQ52_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ86721.1"
                     /translation="MAQETIKAENDPRILVDVRAFLKALNAGGGKPLEQLSPAEARQV
                     LVGPQKSVTVDYSGIVESERSIVQDGITVRIHVVKPEGAAAGGPVFMFFHGGGWVLGD
                     YPTHRRLVRDLVVGSGVTAVFPDYTPSPEARFPVAINEAYAATKWVAAHGAEIGVDGS
                     RLAVVGNSVGGNMAAVVSLMAKDRQGPKISLQVLLWPVTNANFETDSYHDFAVDRFLS
                     RNMMIWFWDNYTTDAAQRADKYASPLLASDSGLKGLPPALVQTAENDVLRDEGEAYAR
                     KLDAAGVAVTLTRYQGLIHDFGLLNPLAHVPAVQVALRQAAVVIKEGLK"
     gene            2901..2982
                     /locus_tag="VKQ52_05770"
     tRNA            2901..2982
                     /locus_tag="VKQ52_05770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2935..2937,aa:Leu,seq:gag)
     gene            3096..>3324
                     /locus_tag="VKQ52_05775"
     CDS             3096..>3324
                     /locus_tag="VKQ52_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86722.1"
                     /translation="MANRLGVHKFYFMSIEITAKVSRNGLKKWYYYEWGKGPGERKAA
                     GVFTYTKPRDIVQKNHNKEALALLETKKSQMV"
BASE COUNT          733 a          746 c         1095 g          750 t
ORIGIN      
        1 acgaggtgcg ttattatccc cttgtgcagc ttttcatcgt ggcgctgttt attttcttta
       61 cgctgtattc cattactgtc cggaacaagg caacgcagaa ccagctctgg gccgggatgg
      121 ccaaggagac ggcgcaccag ctggggacgc cggtatcttc gctggaaggc tgggtggaga
      181 tgctgcggga gagtgccgtg gatccccgga ccgtgcagga gatacaaaag gatgtggacc
      241 ggctgaagct ggtgtccgac cggtttggga agatcggcag caagccggtg ctggaggaaa
      301 aggacctggt cggacaggta cagacgatga tggagtatat ccggaagcgg gcgacgggga
      361 aggtacagtt cgtgctccat gagccggtga cgaccccgct gtatgcccgg atctcgggac
      421 cgcttttcga ctgggtgatc gagaacctgc tgaagaatgc gcttgacgcg atggagggta
      481 aggggtcgat cacgatcggg atcgtcgatc atgagaagga gatcgtgatc gatgtgacgg
      541 atacggggaa ggggatcgcc gcgcgaaacc tgcagaaggt gttcaagccg gggtttacga
      601 cgaaaaaacg cggctggggg ctggggctga gcttatcgaa gcggatcatc gagcagtatc
      661 accaggggca gctgttcgtc cggcagtcgg agcttgggaa ggggacgacg ttccggatcg
      721 tgctgaggaa ataattcatt ttatggaaag atatttcgag cagaattata aaaaactggg
      781 ggtggtggag gtcgatgaag ccgcggtggc ggaggggatc gatgagcagc attacaaatc
      841 ctatatccgg gtcttgtacc tgccggcagg ctattcgctg acggtagact tcaagcgata
      901 tgatacgaag aaggggacgc tcttctttat caattccaac caatacttcc acctggaccg
      961 gcggggtgcg gggatgggat atcttatcta ttataaccgc gacttttatt gtgtgcagat
     1021 ccacgatgag gaggtggcct gtgacgggtt gttgttcaat aatatttacg ggatgccgat
     1081 gacggtgttg ccgccgaagg aggagcggct ggtgtccggg gcgctgcagc atatcctgga
     1141 ggagctgagc caccgggagt cggcgtatga ggaaatggtc cggacctatc tgaagcagat
     1201 gatcatcctt gccacgcgga tctggaaaca gcagcagttg ggtcagctga aggagcagcc
     1261 gggggaggac atcgaattct tccggacctt cagccggctg gtggagatcc atttccgaga
     1321 gaagcatagc gtcaacgagt atgctgagct gctgggtgtc gcggcgaaga ccctttccaa
     1381 gaaattcaac cggctgcggc tgacccagcc gaatgaggtg atcaaggacc gtatcctgct
     1441 cgaggcgaag cggctgctgg tgtattctgc gcttagcata aaggagattg cctacgatct
     1501 tgggtatgaa gaccctgcct atttcaaccg gctgttcacg gcgaaggcgg ggtgtacgcc
     1561 ggcggtgttc aggaaggggc atggagggga gggatgacca tgcgggaggg gaatgctcgt
     1621 gggagggtgg ctccggatgt tggagagaag ccggatgttt gaggggcgcc ggatgcagtt
     1681 tttcccttta ggggaaaaaa gtacaatttt aatacccttt ttgatctttt acagggcctt
     1741 tcggcgagat aattttgtgt cgtacaaaaa acaaatgtat gcaacacaaa acaccgaaag
     1801 caggctttgc tgtcctcttc tggttatttt tcactctaac aacatcttcc attatggcac
     1861 aagaaacgat caaagcagaa aatgatccgc ggatccttgt cgatgtccgc gctttcctga
     1921 aagcattgaa tgccggtggc ggcaagccgc tggagcagtt gtcgcccgcc gaagcccgcc
     1981 aggtgcttgt cggcccgcag aaatccgtta ctgttgacta ttcgggtatc gtggaatccg
     2041 aaagaagcat tgtgcaggat gggatcacgg tgcggatcca tgtggtaaag ccggagggcg
     2101 cagctgcggg cggtcccgtt tttatgttct tccatggcgg cggctgggtt ctgggggact
     2161 accctactca ccggagactg gttcgcgacc tggtggtggg aagtggggtc acggccgtat
     2221 tcccggatta tacgccctcg ccggaggcgc gctttccggt ggccatcaac gaggcctatg
     2281 ccgcgaccaa atgggtggcg gcgcatggcg cggagatcgg ggtcgacggg agccggctgg
     2341 ctgtcgtggg gaatagcgtc ggcgggaata tggcggccgt tgtgtcgctg atggcgaagg
     2401 ataggcaggg gccgaagatc agcttacagg tgctgctatg gccggtgacg aatgccaatt
     2461 tcgagacgga ctcgtatcac gatttcgctg ttgaccggtt cctgagccgt aatatgatga
     2521 tctggttttg ggataattat acgaccgatg cggctcagcg ggccgacaaa tatgcttccc
     2581 ctttgctggc ttccgattcc gggttgaaag ggctgccgcc ggccctggtg cagaccgcgg
     2641 aaaatgatgt ccttcgggat gagggtgagg cctatgcccg gaagctggat gccgccgggg
     2701 tggcggtgac gctgacgaga taccaggggc tgatccatga ctttggcctg ctgaatcccc
     2761 tggcgcatgt cccggcggtg caggtggcgc tgcggcaggc ggcggtggtg atcaaagagg
     2821 ggctgaaata aaaaaagcgt tgaaaatttg atcgaaaagt cctagttttg cagccacttc
     2881 aaaatttccc actggagtat gcgtgggtgg cgaaattggt agacgcacta ctttgaggtg
     2941 gtagcgccgc gaggcgtgtg agttcgaatc tcatcccgcg cactgagctt tctaaataaa
     3001 gcctgtaagt caatgattta caggctttat ttttccccgc gttcgccact ttttgccggc
     3061 ttttttcgct tgtttccgtt ttttttggcg aacaaatggc gaacagattg ggtgttcaca
     3121 aattctattt tatgagcatc gagatcacgg caaaagtgtc acggaatggg ttgaagaaat
     3181 ggtattacta tgagtggggt aagggtcctg gggagagaaa ggcggcgggt gttttcactt
     3241 acactaagcc gagagatatt gtccagaaga accataataa ggaggcgctg gcgcttcttg
     3301 agacgaagaa gtcccagatg gtca
//
LOCUS       DATGFG010000309         7007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_490343, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000309 DATGFG010000000
VERSION     DATGFG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490343"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1110
                     /gene="lpxD"
                     /locus_tag="VKQ52_05780"
     CDS             64..1110
                     /gene="lpxD"
                     /locus_tag="VKQ52_05780"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HLZ86723.1"
                     /translation="MQFSAAQIALLINGKIEGDATATVASFGKIEEAGAGQLAFLANP
                     KYEEHLYTTGASVIIVNATLELKQPVGSTLIRVPDAYSAFASLLTQYQDFEAQQLTGI
                     QEPSYIAASARLGDKVFIGAFAYLGEGVVIGNNVKIHPQVYLGRNVRVGDNTVLHPGV
                     KIYHDCVIGKNVTIHAGTVIGGDGFGFAPQADGTFKKVPQIGNVEIEDFVEIGSNATI
                     DRATMGSTIIRTGAKLDNLIQVAHNVDVGHHTVIAAQAGISGSTKIGSGVMIGGQAGL
                     VGHLQIADGARINAQSGVTKSIKSPGGAVTGTPAYEYTSALRSQALSRNLPELEKRLK
                     ELEEQIKQLLSEKV"
     gene            complement(1245..3020)
                     /locus_tag="VKQ52_05785"
     CDS             complement(1245..3020)
                     /locus_tag="VKQ52_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HLZ86724.1"
                     /translation="MSDRPALPSSADLLSLLRQYWPHKAFRPQQEEIIRSVLDDKDVI
                     ALLPTGGGKSLCYQLPALVRPGLCLVISPLVALMKDQTDSLRRRNITAFALHAGMSRK
                     EVINTLQLAGNSNCKLLYVSPERLESALFIEYLPSLKVSMIAVDEAHCVSQWGYDFRP
                     PYLRIAALREHLPGVPILALTASATLDVLDDIGDKLQLREPCLFRQPFTRSNLSYSFF
                     RVENKFTRITEILTKVAGSSIVYCKTRQATREISEWLIRQGIRAAAYHAGLSQEERSQ
                     RQDDWLKDRTRVIVSTNAFGMGIDKAGVRTVIHADVPESVENYYQEAGRAGRDSEKAY
                     AVLLADGAEAARLEKQPDSRFPPMDQVRPVYQQIVNYLQLPAGAGEGQYYDFDLATFT
                     TRFELTTTTVVNVLKVLEQEGLLSFQQQVFLPAKIQFTTGKEGLYDFERDHPELETLI
                     HALLRTYEGIFDQPVAVRERQLAWNTRKEPAQLAAELARLQAFRIIEYTPAKESPQLY
                     FFRNRPAAEELYINPVQYRERKERYAGRIRAMLRYLGLEEGCRSRYLANYFGDKETGD
                     CGICDNCLRRRPEQAPQNGPSRPRR"
     gene            complement(3026..5158)
                     /locus_tag="VKQ52_05790"
     CDS             complement(3026..5158)
                     /locus_tag="VKQ52_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HLZ86725.1"
                     /translation="METINWKVEGMSCSTCALTIGKYLEKKGLQNIKVSLASGDVSFD
                     AETGVDSTQLQKGIHDLGYTVLKGEAAGAAGGRQPMNKHLRYFLICLPFTALLLLHMA
                     GEGRVPHWLMHPWIQFALCLPVFITGMLFFGRSAVKSLVNGLPNMNVLIALGATAAFV
                     YSLTGTLLRLGPDYLFYETTASIITIVFLGYYVEDASVRATQRTLNSLARTRKTMANM
                     IAFDDRHEEILFPVDSTQLRSGDLILIRSGEMVPADCKILWGEAGADESILTGESLPI
                     DKKAKDKLIGGSLLVAGTVKAQVTAAGDDSVLAGIVRLVKQAQGEKPPVQQLADRISA
                     VFVPVVIGIAVLTFGGNFLFFHRVAPALMRSIAVLVIACPCAMGLATPAAIAVGLGRG
                     ARRGILIRNARHLEAFRHIRQVVFDKTGTLTTGEFSISNWQAVEIAPQRFQEIAFALE
                     KYSNHPIARGITREWKPAVAAQAGVLRWTKVEEVKGLGMRGETKEGEVYWAGSYKVAA
                     HLTADDTHNVYIVANDRLLGFIDVTDTIRPEAATVVRRLQEKGIHTILLSGDRLKPCR
                     QLADTLGIREVLAEQSPEQKLRQVTRLNAEMPVAMIGDGINDAPALARATIGISMSDA
                     SQLAMQTADVVLMNQGLRQLPAALGLGRRTFATIKGNLFWAFAYNILAIPVAALGLLT
                     PAVAALAMGFSDVVLAANSLRLYIKKLD"
     gene            5265..5699
                     /gene="tsaE"
                     /locus_tag="VKQ52_05795"
     CDS             5265..5699
                     /gene="tsaE"
                     /locus_tag="VKQ52_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HLZ86726.1"
                     /translation="MKLDFHLQDIEKAAAAFWQAFPLQRIFAFHGGMGAGKTTFIHAL
                     CAIKGVTDTVGSPTFSIINEYRYPGGRIFHLDLYRLRDEGEALRAGVEDVLYSGEICL
                     VEWPDRAPGIFPPDTAHIRLEMLDPDTRKISWSANSDDQNQF"
     gene            5721..6938
                     /locus_tag="VKQ52_05800"
     CDS             5721..6938
                     /locus_tag="VKQ52_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HLZ86727.1"
                     /translation="MSQQQKPVISTSFSYETLEETLDIKPKGVPLHIGIPKETAFQEN
                     RIALTPDAVGVLVSNGHEVMIEHNAGEASHYRDRDYSEAGARIIHDKSEVLKAPILVK
                     SAPVIEEDLPLLQMNQIIISPIHLSAMKADLLQKMMDKRITGISFENLKDDSGTYPIV
                     RSMSEIAGSAVMLVAGQYLSSANRGKGVLLGGISGIPPTKVIILGAGIVGEFAARAAL
                     ALGASVKVFDNSVYRLKRLQNNIGQRMWTSVMEPRILAKQLKTCEIAVGALSSQTGRT
                     PVVVTDEMVSNMRPGSVIIDVSIDRGGCFETSEITTHEHPVFTKYGVIHYCVPNIPSG
                     FARTASHAISNVLMPLLLEAGEEGGFENLVWHKIHLRSGIYLFKGALTNFHLSQRFDL
                     KYTDLNLLIASQR"
BASE COUNT         1444 a         2086 c         2043 g         1434 t
ORIGIN      
        1 ttttatattt gccatttgaa tacgtcattt taatgttgaa ggtctgttga aaaaccactt
       61 attatgcaat tttctgccgc acaaatagcc ctgttgatca acggcaagat cgagggggat
      121 gctactgcca ccgttgcctc atttggaaag atagaagagg ccggcgcagg gcagctcgcc
      181 ttcctggcca atcccaaata cgaggagcat ttgtatacga cgggcgcatc cgtgatcatc
      241 gtcaatgcga cgctggagtt gaagcaaccg gtaggctcca cccttatccg ggtgccggat
      301 gcctattcgg cctttgccag cctgttgacg caataccagg acttcgaggc gcagcagctg
      361 acgggtatcc aggagccttc ctacatcgcg gcttcggcca ggctggggga caaggtcttc
      421 ataggtgctt ttgcttatct cggtgaaggg gtggtcatcg gcaacaatgt caagatccat
      481 ccgcaggtct acctgggcag gaatgtccgg gtgggagaca acaccgtgct gcatccgggt
      541 gtaaagatct atcatgattg cgtgatcggg aagaacgtga cgatccatgc cggtacggtc
      601 atcggcggtg acggcttcgg ctttgcaccg caggccgatg gaaccttcaa gaaggttccc
      661 cagatcggta atgtcgaaat agaagatttc gtcgagatcg gttcgaatgc cactatcgat
      721 cgggctacga tgggctcgac cattatccgc accggggcaa agctggacaa cctgatccag
      781 gtggcccaca acgtggatgt gggacaccat accgttatcg ccgcgcaggc cgggatcagc
      841 ggcagcacca agatcggcag cggggtcatg atcggcggac aggccgggct tgtcggtcac
      901 ctgcagatcg ccgacggggc ccggatcaat gcccagagcg gcgttaccaa atccatcaag
      961 tcacctggcg gcgccgtgac gggcacgcct gcctacgagt ataccagcgc cctgcgcagc
     1021 caggccctca gccgcaacct gcccgaactg gaaaagcggt tgaaggaact cgaagagcag
     1081 atcaaacagc tcctgtcgga gaaggtctga ccgtccgccc cgctttgaac tcaccttgtc
     1141 accccccctt ttcttgaaaa atgtggtcct tttaatccat ttatcgcccg ccggacctac
     1201 cgctttatca cccgccgggc ctaccgcctt atcgcccgcc agacctagcg gcgtgggcgc
     1261 gaggggccat tctgcggagc ctgttccggc cggcgacgca ggcaattgtc gcagataccg
     1321 caatcgccgg tctccttatc gccaaaataa ttcgccaggt agcggctgcg gcatccctcc
     1381 tccagtccca gataacggag catggcgcgg atgcggccgg cataccgttc ctttctttcc
     1441 cggtattgca cggggttgat atagagctct tccgccgccg ggcggttccg gaagaaatac
     1501 agctgggggg actcctttgc cggggtgtat tcgatgatcc ggaaggcctg cagccgggcc
     1561 agttcggccg ccagctgggc cggttctttc cgcgtattcc aggccagttg ccgctcgcgt
     1621 accgccacgg gttggtcaaa aattccttca taggtgcgca acagcgcatg gatcagcgtc
     1681 tccagctccg gatggtcgcg ttcgaaatcg taaagcccct cctttcccgt agtgaattgg
     1741 atctttgccg gcaggaacac ctgctgctgg aagctcagca gcccctcctg ttccagcacc
     1801 ttcagcacat tgacgacggt ggtggtggtc agctcgaaac gcgtggtaaa ggtcgccagg
     1861 tcgaaatcgt aatattgccc ttcgccggcg ccggcgggaa gctggagata gttgacgatc
     1921 tgctggtaca cggggcgcac ctggtccatc ggcggaaagc ggctgtcagg ctgtttttcg
     1981 agtcgcgccg cctcggcccc gtcggcgagc aggacggcat aggccttctc gctatcccgc
     2041 ccggcgcgac ccgcctcctg gtaataattc tccaccgact ccgggacatc ggcatggatg
     2101 acggtgcgga caccggcctt gtcgataccc atcccaaagg cattggtact aacgatgacg
     2161 cgggtacgat cctttaacca gtcgtcctgg cgctggctcc tttcttcctg cgaaagcccc
     2221 gcatggtagg cagcggcacg gatgccctgg cggatcagcc actcgctgat ctcgcgggtc
     2281 gcctgacggg tcttacaata cacgatgctg ctgccggcca ccttcgtcag gatctcggtg
     2341 atcctggtaa acttgttctc cacccggaaa aaactgtacg acaggttgga tcgcgtaaac
     2401 ggctggcgga aaagacaggg ctccctgagt tgtaatttat ccccgatatc gtccagcacg
     2461 tccagcgtgg ccgatgcagt gagggcaagt atgggtacac cgggcaggtg ttcgcgcaac
     2521 gcggcgatcc gcaggtaggg cggtcgaaaa tcataccccc actgggatac gcagtgggcc
     2581 tcgtcgacgg cgatcatgct caccttcagc gaggggagat actcgataaa aagggcgctc
     2641 tccagtcttt ccggcgaaac atacagcaat ttacaattac tgttccccgc cagctggagg
     2701 gtgttgatca cctccttccg gctcatcccc gcgtgaagcg cgaaagcggt gatattccgc
     2761 cggcggagac tgtcggtctg atccttcatc agggcgacaa gcgggctgat cacgagacaa
     2821 agcccgggcc gcaccagggc cggcagctga taacaaaggg acttgccccc acccgtggga
     2881 agaagggcaa tcacatcttt gtcatccagc accgagcgga tgatctcctc ctgttgcggc
     2941 cggaaggcct tgtggggcca atattgccgg agcagggaaa gcaggtcggc ggacgagggt
     3001 aaagcagggc ggtcagacat gagcgttaat ccagtttttt tatatagagc cggagggagt
     3061 tggcggccag gacgacatcg ctgaacccca tcgccagcgc ggccacggcg ggtgtcagca
     3121 aaccgagggc ggctaccggt atcgccagga tattatacgc gaaggcccag aacaggttgc
     3181 cttttatcgt ggcaaaggta cgacggccaa gaccgagtgc cgccggtaat tgccgcaacc
     3241 cctggttcat cagcacgaca tccgccgtct gcatggccag ctgcgaggcg tcgctcatcg
     3301 agatgccgat ggttgcccgg gcaagggccg gggcgtcgtt gatcccgtcc ccgatcatcg
     3361 ccaccggcat ttcagcgttg agccgggtca cctgccggag cttttgttcc ggcgattgtt
     3421 cggccagcac ttcccggatg ccgagcgtat cggccaactg cctgcagggc ttgagccggt
     3481 cgccgctcag cagaatggta tggatccctt tctcctgcaa ccggcgcacc acggtcgccg
     3541 cttcggggcg gatagtgtcg gtgacatcga taaaccccag caggcgatcg ttggcgacga
     3601 tatagacatt atgggtgtcg tcggcagtaa gatgcgccgc gactttatac gaaccggccc
     3661 agtacacctc gccttccttt gtctcgcccc gcatgccgag gccttttacc tcttccacct
     3721 ttgtccagcg caggacgccg gcctgagccg caactgccgg cttccattcc cgcgtgatcc
     3781 ctctcgcaat ggggtggttg gaatattttt ccagcgcgaa ggctatttcc tggaatcgct
     3841 ggggcgcgat ctccacggcc tgccagttgc tgatcgagaa ctcccccgtc gtcagcgtgc
     3901 cggtcttatc aaagaccacc tgccggatat gccggaaggc ttccaggtga cgggcgttgc
     3961 ggatcaggat gccccttcgc gcgccccggc ccagaccaac cgcgatggcg gcgggagtag
     4021 ccagacccat ggcgcaggga caggcgatga ccaggacggc gatgctgcgc atcagcgcgg
     4081 gcgccacccg gtggaagaaa agaaagttgc cgccaaaggt cagcacggca atcccgataa
     4141 cgacgggtac gaagacggcg ctgatcctgt ccgccaattg ctgcaccggc ggtttctcgc
     4201 cctgggcctg ctttaccagc cggacgatac ccgcaagaac ggagtcgtcg ccggcggccg
     4261 taacctgtgc ctttaccgtt cccgcgacga ggaggctgcc gccgatgagt ttgtcttttg
     4321 ccttcttgtc gatgggcagg ctttcgcccg tgagaatgga ttcatcggcg cccgcttcac
     4381 cccacaggat cttacagtcg gcagggacca tctcaccgct gcggatcagg atgagatcgc
     4441 cgctccgcaa ctgggtgctg tcgacgggaa aaaggatctc ctcatgccgg tcgtcgaagg
     4501 cgatcatatt ggccatggtc ttccttgtcc tggccaggct gttcagcgtc cgctgggtag
     4561 cccgcacgga agcatcttct acgtaatacc cgagaaagac gatggtgatg atggaggccg
     4621 tggtctcata gaaaagatag tccggcccca aacgcagcag ggtgccggtg aggctgtaga
     4681 caaatgccgc cgtggccccc agcgcgataa ggacattcat attgggcagc ccgttgacca
     4741 ggctcttgac cgcactgcgg ccaaagaaga gcatcccggt gatgaatacc ggcaggcaaa
     4801 gcgcgaattg gatccaggga tgcatcagcc agtgcggcac acgcccctcc cccgccatgt
     4861 gcaggagaag cagggcggtg aagggcagac agatcaggaa ataccgcagg tgtttgttca
     4921 tgggttgccg gccaccggcc gctccggcgg cttctccctt cagaaccgta tacccgagat
     4981 catgaatgcc cttttggagc tgggtgctgt cgaccccggt ttcggcatcg aagctcacgt
     5041 cgccgctggc gaggctgacc tttatatttt gcagaccctt tttttccagg tatttcccga
     5101 tggtgagtgc acaggtggag cagctcatcc cttccacttt ccaattaatg gtttccatat
     5161 aattatgtgc gcggccctcc gccggcaaag cccaaagtta ctgtatcttt gcggccctgt
     5221 tactgtatct ttcccgccct cttactgtat ctttgccacc ctatatgaag ctcgattttc
     5281 acctgcagga catagagaag gctgccgccg cgttctggca agcctttccc ctgcaacgga
     5341 tctttgcctt tcatgggggt atgggagcag gcaagacgac ctttatccat gccctttgcg
     5401 cgatcaaagg ggtcaccgac accgtgggaa gtcctacttt ttccatcata aacgaatacc
     5461 ggtatccagg tggcaggatc ttccatctcg atctttaccg gctgagggac gagggagaag
     5521 ccctgcgggc cggggtggaa gatgtcctgt acagcggaga aatatgcctc gtggagtggc
     5581 cggaccgggc ccctggtatt tttccccccg ataccgccca tatccggctc gaaatgctcg
     5641 acccggatac ccgtaaaatc tcctggagcg cgaattccga tgatcaaaat caattttaaa
     5701 ttgtaatttg acaggcatat atgtcgcagc agcaaaaacc agttatcagc acctccttca
     5761 gttacgaaac cctggaagaa accctggaca tcaagcccaa gggggttccc ctccatatcg
     5821 gcatcccaaa ggagaccgcc ttccaggaga accgcatcgc cctgacgccg gatgcggtgg
     5881 gcgttctggt aagcaatgga catgaggtga tgatcgaaca taatgccggc gaagcttcgc
     5941 actaccggga cagggactat agcgaggccg gggcaaggat catccacgat aaatcggagg
     6001 tgttgaaggc tcccatcctc gtcaaaagcg cccccgtgat cgaagaggac ctgccgctgc
     6061 tgcagatgaa ccagatcatc atctccccca tccatctttc cgcgatgaag gccgatctgc
     6121 tgcagaagat gatggataag cggatcaccg gcatctcttt tgaaaacctg aaggacgaca
     6181 gcggaaccta tcccatcgtc cgcagcatga gcgagatcgc cggcagcgcg gtgatgctgg
     6241 tcgccgggca ataccttagc tcggccaacc ggggcaaggg ggttctgctg ggaggcatct
     6301 ccggtatccc gccgacaaag gtcatcattc tcggggcggg tatcgtcggg gagtttgcgg
     6361 cgcgtgcggc ccttgcgctg ggcgcttccg taaaggtctt cgataacagt gtgtaccggt
     6421 taaaacgttt acagaacaat atcgggcagc ggatgtggac ctcggtgatg gaaccgcgca
     6481 tcctcgccaa acagctcaag acctgtgaga tcgccgtcgg cgccctttcc tcgcagacgg
     6541 gccggacacc cgtggtcgtc accgacgaga tggtgagcaa tatgcggccc ggttcggtga
     6601 tcatcgacgt cagcatcgat cgcggcggct gcttcgagac ttccgagatc actacccacg
     6661 aacatcccgt ctttaccaaa tacggcgtca tccactactg tgtccccaat atcccttcgg
     6721 gattcgcccg tacggcctcg cacgcgatca gcaatgtgct gatgccgctg ctgctggagg
     6781 cgggtgagga aggcggcttc gagaatcttg tctggcataa gatccacctg cggagcggca
     6841 tctatctctt caagggagcc ctgaccaatt tccatctcag ccagcgtttc gacctgaagt
     6901 ataccgacct gaacctgctt atcgcgagtc agcggtagca tctacggatg aggagaaaga
     6961 aggggcagca cctcccggtc aggctttact tccaatccaa aatactg
//
LOCUS       DATGFG010000310         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_146112, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000310 DATGFG010000000
VERSION     DATGFG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146112"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VKQ52_05805"
     CDS             <1..1087
                     /locus_tag="VKQ52_05805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLZ86728.1"
                     /translation="FYQTYFIYLFAQYLLTLTGRITILNIVRGQVRRGKVVFNALLVG
                     CDDVATKIYKDTWEALSSSGYRYKGYVTNRNQDHRRNGIAEYLVDLGDSEEIEKTIDQ
                     HDIELVVVALEASAREEVGAIVEKLSNKDVRIKIIPSTLDILSGSVRTNNVQEAILSD
                     IYTSPMPEWQQNIKRIIDVVISVAGLILLSPLMLYAAIRVRLSSAGPVIYQQERIGFK
                     GKKFQIYKFRSMIADAEKDGPALSSLQDPRITPWGRTMRKWRIDELPQLWNILKGEMS
                     LVGPRPEREYYVDQLYLHTPYFRYLLKVKPGLTSWGMIQFGYAENIDEMVHRMKYDLM
                     YLENISLALDLKIMVHTLRIIFTGQGK"
     gene            1112..3886
                     /locus_tag="VKQ52_05810"
     CDS             1112..3886
                     /locus_tag="VKQ52_05810"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLZ86729.1"
                     /translation="MPKTLGLLWLATWLFCLSARPQAAHTTPSPHPYWQQRVDYTIDV
                     TLDDREKSLDGFVRMEYTNNSPDTLAFIWIHCWPNAYKNDRTAFSEQLLGNGRTDFYF
                     SDKQQRGYINRLDFRADGQEAKMEDHPQYIDVIKVVLPKPLPPGGHTTLTTPFHEKLP
                     YNFSRGGWTLTPSAPQHHPFGKPPSTPAAPPTVSYQVTQWYPKPAVYDSRGWHPIPYL
                     DQGEFYSEFGDFDVRITAPNNLIVAATGESQDTSYPSNSTRTLHYKQTRIHDFAWFAD
                     RRFHVDHDTLRLPSGRTIDVYSYYTPTAPDGWTNSTTYIKDAVRFRSALIGEYPYNTV
                     TAVQAKMGATGGMEYPTITAINSSGPARDVDLTIEHEVGHNWFYGILGSNERRYPWMD
                     EGINTYYDSRYEALKYPSEGDVNYPSIGTLAVQKKDQPISTPSEDFTEENYFNIAYTK
                     TGSWMKMLEDTLGTALFDRCMQEYFHEWQFKHPWPDDFRAVITNTSHRSLDTLFALLD
                     KKGALPPLPAHRKLKPALLFNLTQTDKYNYLGLFPAFGYNEYDHFMAGLLIHNFNLPP
                     NPIQFYLAPLYATGSHQLNGTGSLIWSLYPDHGLQKIQGLLGIQRFSTMSGTDSSGGR
                     LTGGYYKITPGLRIVFPKSSPRSTLETSLEWKTFLIGEKNLDHFVRKTADSLYYPVAG
                     KYDFRYLNQLSFDLRDTRVLYPYNVRLQLQQAASWYRVNFTTNYFFNYAKGGGLDLRV
                     FGAKFGYLGSRSPTEDLSRFEPKLTAVRGDEDYTYNNYFIGRNEFMGGASQQVLMRDG
                     DLKIRTDLFQGLQGRSDNWVASINLRTTLPRAIVPEWLPLRAFFDAGTYSGAWENSPP
                     TSHFLYVGGFELAILHDVIRIYAPLVYSSDFSSQLKTVPDQNTFWKRISFSVDLQNID
                     FRKLFGNMPF"
     gene            3886..>4517
                     /locus_tag="VKQ52_05815"
     CDS             3886..>4517
                     /locus_tag="VKQ52_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86730.1"
                     /translation="MSTGPHIRYLKRQDIDPVRWDNCVKWSPNGWLYLHTFFLDAWGP
                     WDALVAHTPQHGEDYACIMPLPKKRKYGLTYTYILPLPGQLGIAGILPLTAHLTNEFL
                     RRIPPSFRLVDTLLNEGNTPPSLPGVEVIPRTNYVLPLNTDYPALYARFNDDAKKNLR
                     QAYAKGLYPDTDIPVEQVIHLYHAAYGNRISHLSAKSYRQIAQLCRECME"
BASE COUNT         1096 a         1637 c          965 g          819 t
ORIGIN      
        1 tttttatcag acctatttca tctatctctt tgcccaatac ctgctcaccc tcaccgggag
       61 gatcaccatc ctcaacatcg tccgcggcca ggtacgccgg ggaaaagtgg tcttcaatgc
      121 gctactggtg ggctgcgacg acgtcgccac aaagatctac aaggacacct gggaagccct
      181 gtcttcctcc ggctaccgct acaaaggcta tgtcaccaac cgcaaccagg atcatcggcg
      241 caacggcatc gccgaatacc tcgtcgacct cggcgactcc gaagaaattg aaaaaaccat
      301 cgaccaacac gacatcgagc tcgtcgtcgt tgcactcgaa gccagcgcaa gggaagaagt
      361 aggcgccatc gtcgaaaagc tgagcaacaa ggacgtccgg ataaagatca tccccagcac
      421 actggacatc ctgtccggat cggtgaggac gaacaacgtc caggaagcca tcctctccga
      481 catctatacc agcccgatgc ccgaatggca gcagaacata aaacgcatca tcgatgtcgt
      541 catctccgtg gccggcctga tcctcctctc ccccctcatg ctctacgccg ccatccgcgt
      601 ccgcctctcc tccgccggcc ccgtcatcta ccaacaggaa aggatcggct tcaaagggaa
      661 gaaatttcag atctataaat tccgctccat gatcgccgac gcggaaaaag acggccccgc
      721 cctctcctcc ctccaggacc cacgcatcac gccctgggga cgaacgatgc gcaaatggcg
      781 tatcgacgaa ctcccgcaac tgtggaatat cctgaaaggc gaaatgagcc tggtaggccc
      841 ccggcccgaa agggaatatt atgtcgatca actatacctg cataccccct atttccgcta
      901 cctcctgaaa gtaaaaccag gcctcacctc ctggggaatg atccagttcg gctacgccga
      961 gaacatagat gaaatggtcc atcgcatgaa atacgacctt atgtacctcg agaatatctc
     1021 cctcgccctc gacctcaaga tcatggtgca cacgctccgg atcatcttca ccggccaggg
     1081 aaaataaaag aatagcttac ttttgatgaa cgtgccaaaa acattaggac tactgtggct
     1141 cgccacctgg cttttctgcc tctccgcacg gccccaggcc gcgcacacaa ccccatcccc
     1201 ccatccctac tggcaacaac gggtggatta caccatcgac gtcaccctcg acgaccggga
     1261 gaaaagcctc gacggcttcg tccgcatgga atacaccaac aactcccccg acaccctggc
     1321 ctttatctgg atccactgct ggccaaatgc ctataagaac gaccgcaccg ccttcagcga
     1381 acaactgctc ggcaacggcc gcaccgattt ctatttctcg gacaaacaac aacgcggcta
     1441 tatcaatcgc ctcgacttcc gcgccgacgg ccaggaagca aaaatggaag accaccccca
     1501 atacatcgac gtgataaagg tcgtcctgcc aaaacccctg cccccgggcg gacacaccac
     1561 cctcacgacc cccttccacg aaaagcttcc ctacaatttc tcccgcggcg gctggacgct
     1621 cactccctcc gccccccaac accatccatt cggaaagccc ccttccaccc ctgcggcgcc
     1681 cccaaccgtc tcctatcagg taacccaatg gtaccccaaa cccgccgtat acgatagccg
     1741 cggctggcac cctatcccct atctcgacca gggtgaattc tacagcgagt tcggcgattt
     1801 cgacgtccgc atcaccgccc ccaacaacct catcgtcgcc gcaaccggcg aatcacagga
     1861 taccagctat ccctccaaca gcacaagaac cctccattat aaacaaaccc gcatccacga
     1921 cttcgcctgg ttcgccgacc gccgcttcca cgtcgaccac gacaccctcc ggctcccctc
     1981 cggccgcacc atcgacgtct actcctacta taccccgaca gcgcccgacg gctggacaaa
     2041 cagcaccacc tacataaaag acgccgttcg cttccgctcc gccctcatcg gcgaataccc
     2101 ctacaacacc gtcaccgccg tacaggcaaa aatgggcgcc acgggaggga tggagtatcc
     2161 gaccatcacg gccatcaata gctccggacc ggcccgcgat gtcgacctca ccatcgaaca
     2221 cgaggtcggt cacaactggt tctacggcat cctgggctcc aacgaacgcc gctacccctg
     2281 gatggacgaa ggcatcaaca cctactatga cagccgctac gaagcgctga aatacccgtc
     2341 cgaaggcgac gtcaactacc cttccatagg cacgctcgcc gtacagaaaa aagaccagcc
     2401 catctcgacg ccatccgaag acttcacgga agaaaactat ttcaacatcg cctatacaaa
     2461 gaccggcagt tggatgaaaa tgctggaaga taccctcggg acagccctct tcgaccgctg
     2521 catgcaggag tacttccacg agtggcaatt caaacacccc tggcccgacg acttccgggc
     2581 cgtgatcacc aacaccagcc accgctccct cgataccctc ttcgccctcc tcgacaaaaa
     2641 aggggcactc cccccactgc cggcccaccg caaactaaag ccggccctcc tcttcaacct
     2701 tacacaaacc gataaataca actacctcgg cctcttcccc gccttcggct ataacgaata
     2761 cgaccacttc atggccgggt tactgatcca caacttcaac ctgccgccaa accccatcca
     2821 gttctacctc gcacccctct acgccaccgg cagccatcaa ctcaacggca ccggcagcct
     2881 catctggtcc ctctaccccg accatggtct gcaaaagatc cagggactcc tcggcataca
     2941 acgtttctcc accatgagcg gcacggacag cagtggcggc cggctcaccg gcggttatta
     3001 taagatcacc cccggcctcc gcatcgtctt cccaaaatcc tctccccgca gcacgctgga
     3061 aacatccctc gaatggaaga ccttcctcat cggggaaaag aacctcgacc atttcgttcg
     3121 gaagacagca gactcgctct actatcccgt cgccggcaaa tacgattttc gctacctcaa
     3181 ccagctcagc ttcgatctcc gcgacacccg cgtcctctat ccctataacg tccgcctcca
     3241 gctccagcag gccgcctcct ggtacagggt caattttacc accaactatt tcttcaacta
     3301 tgcaaaaggc ggcggactcg acctccgggt ctttggcgcc aaattcggct acctcggctc
     3361 ccgctcaccc accgaagacc tcagccgctt cgaaccaaaa ctcaccgccg tacggggcga
     3421 tgaagactac acctacaaca attacttcat cggtcgcaat gaattcatgg gcggggcaag
     3481 ccagcaggtc ctgatgcgcg acggcgacct gaagatccgc accgatctct tccagggact
     3541 ccagggccgt tccgataact gggtcgcctc catcaacctc cgcaccaccc tgccccgcgc
     3601 catcgtcccc gaatggctgc ccctgcgagc cttcttcgac gccggaacat atagcggggc
     3661 ctgggagaac agtcccccca ccagccactt cctctatgta ggcggattcg aactggccat
     3721 actgcacgat gtcatccgca tctatgcccc cctggtttac agcagcgact tctccagcca
     3781 gctaaagacc gtacccgatc aaaacacctt ctggaaaaga atatccttta gtgtcgacct
     3841 gcaaaacatt gacttccgga agctttttgg taatatgccg ttctgatgag taccggtccc
     3901 catatccgct atctgaaaag acaggacatc gatcccgtcc gctgggacaa ctgcgtaaaa
     3961 tggtctccca acggctggct ctacctgcac accttcttcc tcgacgcctg gggcccctgg
     4021 gacgccctgg tcgcccatac tccgcaacac ggcgaagact atgcctgcat catgcccctg
     4081 ccaaaaaaaa ggaaatacgg gcttacctac acctatatcc tgcccctgcc cgggcaactg
     4141 ggcatcgccg gtattctccc gctcaccgcc cacctgacaa acgaattcct gcgccggatc
     4201 cccccttcct tccggctcgt cgataccctg ctgaatgagg gcaatacgcc gccctccctg
     4261 ccgggggtgg aggtcattcc ccgaaccaat tacgtccttc ccctgaacac ggactacccc
     4321 gctctctacg cccgcttcaa cgacgatgca aaaaaaaacc tccgccaggc ctatgcgaag
     4381 ggactatacc cggatacgga tatccccgtg gagcaggtca tccacctcta ccacgcggcg
     4441 tatggcaaca ggatcagcca tctgtccgca aagagctatc ggcagatagc ccagctatgc
     4501 cgggaatgta tggagaa
//
LOCUS       DATGFG010000311         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1032762, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000311 DATGFG010000000
VERSION     DATGFG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032762"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VKQ52_05820"
     CDS             complement(<1..333)
                     /locus_tag="VKQ52_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86731.1"
                     /translation="MADSHNLIVAVLAKYALGQSLSEEELRLLKEWQERSADRRTLPD
                     QLRDPLWREEHRRELEEAPSAAMWENIRRHIRESKESVEPVSVPGRGYQPSRRWRYVA
                     AAGVIVVAG"
     gene            complement(502..1080)
                     /locus_tag="VKQ52_05825"
     CDS             complement(502..1080)
                     /locus_tag="VKQ52_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ86732.1"
                     /translation="MSNLFQQFASGSEEAFVVYYQRYHKSIYASIKRLCGDDALAEDL
                     TQEVFKNLWDRRAELNDEDHLRNNLFLMARSYFLDNRRRRKIHARAEEELRRTAEPGD
                     DIELTLVTEEVFAIVEGTMMKLPPQQKMVLELLLLRGLDVRAVAKRMQLAPQTVRNHK
                     SQAIHFLRTELYRLDLSLAVLLIILLLCFYEI"
     gene            1698..2330
                     /locus_tag="VKQ52_05830"
     CDS             1698..2330
                     /locus_tag="VKQ52_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ86733.1"
                     /translation="MQALKPEQPVHGNEEAIIISFREGNKEAFRVVYDRMARPLLYFV
                     ENIIHSEPDAEDIVANAFYKLFHSRNGMKSFEHIKRWLYVIVRNEAIDYLRLKTRQRE
                     SQNDLAYLDPGVEEHIETERVRTILLQDIHREIEKLPRQRKTILRLYFFEQKNTSEIA
                     EIMQLSPQTVLNHKTKALDALRKSGLKVRWLLDGVPSFLFAIAFAFYLLK"
     gene            2463..>2902
                     /locus_tag="VKQ52_05835"
     CDS             2463..>2902
                     /locus_tag="VKQ52_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86734.1"
                     /translation="MEQSIDIDPIVTKYLREEALSAEEQTALDNWISQGQGRSQMLEN
                     LKNDTSSTKANLLQMEHIPHSRIWDTLVTRLQQDGYWREEEATAAPMVHAMPSTTRRT
                     RWRYVMAASVLLIAAGSAWWGLHRSPAASTEVVARPAQPVDVAPG"
BASE COUNT          720 a          800 c          719 g          663 t
ORIGIN      
        1 cccggcgacc acgatcaccc ccgccgccgc aacatagcgc catctccggg acggttgata
       61 cccgcgtcct ggaacggaca cgggctctac ggattctttg ctttcccgga tatgccggcg
      121 gatgttctcc cacatcgccg ccgaaggcgc ttcctccaat tcccgccggt gttcctcccg
      181 ccagagcgga tcgcgcaact gatcgggcag cgtccggcga tcagccgaac gctcctgcca
      241 ttccttaagc agacgcaact cctcctcact gagggattgc cccagtgcgt acttggcgag
      301 gaccgctaca ataaggttat gactatcggc catatggact aaggagatta ctggcttctc
      361 atgacaagac aaggaaaaac agatatttgc cgctaatcat cgcttccgga tatagaatgc
      421 atgagacggg cgattcaacc agcgaaaagt caactatttt taggctggac cggggtaatc
      481 tcgtgagagg cccgtcgaat ctcagatctc gtaaaaacac aggagcagga tgatgagcag
      541 cacggcaagg gagagatcaa ggcgatagag ttccgtccgc aggaagtgga tcgcctgcga
      601 cttgtgattg cgcaccgtct gtggcgccag ttgcattctt tttgctacgg cccgcacgtc
      661 cagtccgcgc aggagcaaca gctccaatac catcttttgc tgggggggca atttcatcat
      721 cgtaccttcc acgatggcga acacctcttc cgtcaccagc gtcagctcga tatcgtcgcc
      781 cggctccgcc gtccggcgca gctcctcttc ggccctggca tgaatcttcc gccggcgccg
      841 gttatcgagg aaataactcc gcgccatcag gaagaggtta ttgcgtaaat ggtcttcgtc
      901 gttgagttcg gcccgccggt cccacaggtt cttgaacacc tcctgggtca ggtcctccgc
      961 cagggcatcg tcgccgcaga ggcgtttgat gctggcgtaa atgcttttgt gataccgttg
     1021 ataatagaca acaaaggctt cttcggagcc tgatgcaaat tgttggaaca ggttgctcat
     1081 gctggctgtt ttggttaagt gcccggacac cggtattaga ttaaataaat acgcttacta
     1141 cccaggtttc taacgcaaaa cagacgggat caaccaccgt cactgcagtc ctacatcagc
     1201 cgggtcttcc ccgggatgga tataagtgga tgataaataa catattaaat agaaagtaaa
     1261 accctgatgg caggggcatc ttttttagga gatcaggaca aattttgtat ctttagggga
     1321 cttatctcac ctcgtcctcg cttccaacct tgcataaacc atcgtttcac aggatcgacc
     1381 gttatctacg ggaatacccg tattttttta ggcgggaagt agtttttcga tgaaaaggga
     1441 ttagtttcag tatgaagttt tgcacaaaaa cgcaatttta taccggttcc tttgcgaaag
     1501 atcgcggaaa tggatttatt tgcattgcga cgggccccag cccttcctgc atgagagtta
     1561 aattctatta gaaccacgat tgctgccacc tgctttctcg cctacgaaga aagtcctaaa
     1621 cttttatcct gtttgattat tagccatttg ctgttaggag actaacaaaa tgtaaatccg
     1681 tatttatttg tttaggcatg caggcactaa aaccagaaca accggtccac ggtaacgaag
     1741 aggcgatcat catctccttc cgggaaggca ataaagaagc cttcagggtg gtttatgacc
     1801 gtatggcccg tccgttgctg tatttcgtgg aaaatataat ccactccgag cccgacgcgg
     1861 aagatatcgt ggccaatgcc ttctacaagc tcttccattc ccgcaacggg atgaaatcct
     1921 tcgaacatat caaacgctgg ctttatgtca tcgttcgcaa cgaggcgatc gattatctcc
     1981 gtcttaagac acgccagcgg gaaagtcaga acgatctcgc ctatctcgac cccggcgtcg
     2041 aagaacatat cgaaaccgaa cgggtcagga cgatcctcct ccaggacatc caccgggaga
     2101 tagagaaatt accccgccag cgaaagacga tactgcggtt atatttcttt gaacaaaaga
     2161 atacctcgga gatcgccgag atcatgcaac tcagtcctca aaccgtcctg aaccataaaa
     2221 ccaaggcgct cgacgctctc cgcaaaagcg gtctgaaagt cagatggctg ctcgatggcg
     2281 tcccgagctt cctttttgcc attgccttcg ccttttacct attgaaataa gcagagttaa
     2341 tgtaaattta atgaaatctt aaccgggcta attggttaaa acagcaacca tttcgtgatg
     2401 gcccagagta agatattacc aaacgccaat tgggcaagga taaagaaaac agcaaagcgg
     2461 ccatggagca atcaatcgac atcgatccta tcgtaaccaa atatttgcgc gaagaagccc
     2521 tctcggcgga ggagcagact gcccttgaca attggatcag ccagggacag ggtaggagcc
     2581 agatgctcga gaacctcaag aatgatactt cctccacaaa ggccaacctg ttacaaatgg
     2641 agcatattcc ccactcccgc atctgggata cgctggtcac ccgcctgcag caggatggct
     2701 attggcgcga agaggaggcc actgcggccc ccatggtcca cgccatgcca tccacgacgc
     2761 gccggacccg ctggcggtat gtcatggctg cctccgtatt gctgatcgcg gccggcagcg
     2821 cctggtgggg cctccaccgt tcaccggcgg cttccaccga agtagtggcc cggccggccc
     2881 agcccgtgga tgtcgcaccc gg
//
LOCUS       DATGFG010000312         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_830535, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000312 DATGFG010000000
VERSION     DATGFG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830535"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2561
                     /locus_tag="VKQ52_05840"
     CDS             <1..>2561
                     /locus_tag="VKQ52_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HLZ86735.1"
                     /translation="RGLPDDFSGGTDTIKKVSSVKPNNSWGVTAGADVELTGLPLSLG
                     ASLGVFHTTYTGWGYESAINASINAGSKSTGTLSGGLSLTNNSQNGVTVNPSLGYHYQ
                     YLSAGDNGSASAGVQFSAPYNSRTGLKDIHMGITASVNKQVATGRKDEVGNPIKFGSG
                     TSMDVAGISFAWPSYTPTINMPMTNYNYSFTAKLGGEATVVHPSFYVSGYFGSEYVAG
                     ADTALLIPAYGYLNFQNRSGNWAALTDFNREKELPYRESPPVPHIAVPSYTYDIFNIS
                     GEGTGGMFRAYRGDIGFIADHLISTKTKSGAASVDLGGGDLVHGGVDLNGNYSVTQTG
                     PWLNENPLRNTINFRPSNGLYEAAYFRNPGEKAINTTDFYNAIGGDHVAVPVLNQNGS
                     SIMTTNLLNLFDGPKQVGVDTLTVSNVVKNTRDKRAEVITYLTAQEAAVSGLDKYINR
                     YTINQFADTCDDETVVEPVGIGTGVNSWFYTNMSLSGAPYPYVPLPRLDTTLVFNWGG
                     GSAMYNHSGSNTETQVNIDRNFPHEKYSGRWLGRLKAPVTGVYGFGIYSDDGVRLWIN
                     DSLKINDFTIHGLTWDTCQVNLVAGKLYDIKIEYFQDLRANVMQFAWRRPDRPNEPFY
                     KDHKDSIQIPNIYLPKYSDTIPVNPYLTREDRVNSFRKGNHISEVDVLNPDGKRYVYG
                     IPVYNFVQKEVSFSVDAGEGNIQAGLTSYTDAEASTRNTSGKEGYYSREEMPAYAHSF
                     LLTGILSSDYVDVTGDGISDDDIGDAVKFSYSKTSGVANPYGWRAPYVTGKANYNEGF
                     RSYSRDDKGHYIYGTKELWYLHTIESKTMIATFTLAPRSDQMEIDEHGNKDSSGK"
BASE COUNT          614 a          617 c          728 g          602 t
ORIGIN      
        1 tgcgggggct accggatgat ttcagcgggg ggacggatac gataaagaag gtgtcttccg
       61 taaagcccaa taacagttgg ggggtaacgg ccggtgcgga tgtggagctg acgggtcttc
      121 ccctcagtct gggtgccagc ctgggggttt ttcataccac gtatacagga tggggatacg
      181 agagcgctat caatgcgtcg atcaatgccg gctccaaatc tacgggaacg ctgtcgggtg
      241 gattgagcct gacaaacaat tcgcagaatg gggtcaccgt gaatcccagc ctggggtatc
      301 attatcaata cctgagcgcc ggggacaatg gcagtgcttc cgccggggtc cagttctccg
      361 cgccgtataa ttcgcggacg ggtttgaagg atatccatat gggtattact gcatccgtga
      421 acaagcaggt ggcgaccggc cggaaggatg aggtgggtaa ccctattaaa ttcggctccg
      481 gcaccagtat ggacgttgcg gggatatcgt ttgcgtggcc gtcttatacg ccgacgatca
      541 atatgcccat gaccaactat aattattctt ttacggcgaa attgggcggt gaggctacag
      601 tggtacatcc cagtttttat gtcagtggct attttggtag tgaatatgtg gccggtgccg
      661 atactgcctt gctgatccct gcttatgggt atctgaattt ccagaacagg agtggtaatt
      721 gggcagccct gacggatttc aaccgggaaa aagagctgcc ttatcgggag tctcctcctg
      781 ttccacatat cgctgttcct tcttacacct acgatatttt caacatttcc ggagaaggta
      841 cgggtggcat gttccgggct taccgggggg atatcgggtt tattgcggat cacctcatca
      901 gtaccaaaac caaatcggga gcagcgagtg tggatcttgg cggtggggac ctcgtgcacg
      961 gcggcgtcga cttaaatggg aactattcgg tcacccagac cggcccctgg ttgaatgaga
     1021 atcctcttcg aaatacgatc aatttccgtc cttccaatgg attatatgag gcggcttatt
     1081 tccgcaatcc gggggaaaag gccattaata caacggactt ttataatgct atcggcggcg
     1141 atcatgtggc ggtgccggtg ttgaaccaga acggatcgtc gatcatgacc acgaaccttt
     1201 tgaatttatt cgacgggcca aagcaggttg gggttgatac gcttacggtt tccaatgtgg
     1261 taaaaaatac ccgggacaag cgggcggagg tgatcaccta cctgacggcg caggaggcag
     1321 ccgtatccgg gctggataaa tatatcaacc gctatacgat caaccagttt gccgacacct
     1381 gtgatgatga aacggttgtc gagcctgtcg gcataggaac gggggtcaac agctggtttt
     1441 atacgaacat gagtctttcc ggggcgcctt atccgtatgt gcccttgccc agactggata
     1501 ctactttagt cttcaactgg ggcggcggca gtgcgatgta caatcactcc ggcagcaata
     1561 cggagaccca ggtgaacatc gaccgcaatt tcccacacga aaaatattcg gggcgctggc
     1621 tcggacggct aaaggctccc gtgacgggtg tttacggttt cggcatttac agcgacgacg
     1681 gtgtccgact atggatcaac gatagcctga aaatcaacga cttcaccatc catggcctta
     1741 cctgggatac ttgccaggtg aacctggttg cggggaagct ctacgatatc aagatcgaat
     1801 acttccagga tctgcgggcg aatgtcatgc aattcgcctg gcgccggcct gaccggccca
     1861 atgagccctt ttataaggat cacaaggatt ccattcagat ccccaatatc tatctgccga
     1921 aatactcgga tacgataccc gtcaatcctt atctgacaag ggaggatcgg gtcaactctt
     1981 tccgcaaggg caatcatatt tccgaggtgg atgtcctcaa ccccgacgga aagcgatatg
     2041 tatatggcat cccggtgtac aatttcgtgc aaaaggaagt cagcttttcc gtggatgctg
     2101 gtgaaggaaa catccaggcc gggcttacct cctatacgga tgccgaggct tccactagaa
     2161 atacaagcgg gaaggagggg tattacagca gggaggagat gccggcttat gcccactctt
     2221 tcctgctaac gggcattttg tcttcggatt atgtcgatgt tacgggggat ggcatcagtg
     2281 atgatgatat cggcgatgcg gtgaaattca gctactcgaa gaccagcggg gttgccaatc
     2341 cttatggctg gcgcgccccc tatgtgacgg gtaaggcgaa ctataacgaa ggtttccgca
     2401 gttatagccg ggatgataaa ggacattata tctatggcac caaggagctg tggtacctgc
     2461 ataccatcga atccaagacg atgatcgcca cctttaccct ggcgcccaga tcggaccaga
     2521 tggaaataga tgaacacggg aataaggata gctccgggaa g
//
LOCUS       DATGFG010000313         9216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_599550, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000313 DATGFG010000000
VERSION     DATGFG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9216
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599550"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="VKQ52_05845"
     CDS             complement(<1..2351)
                     /locus_tag="VKQ52_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86736.1"
                     /translation="MFSCDHLRHPFQNDPGTSQGDRAPADLLGDTTPIDGRQLSDLLN
                     YFTALAGQINFYNSDLSVGSWKPFFSNSLPFLLSSISSYSGDSVKARMDDVVRLFRKQ
                     PTPDGLQLLFYQTYYTAIYPLQQWSLGLAGSGLALEQGLQTLIRDRLVPSIKQYISHA
                     NTAAHCFGIQAPAAVELQANAAWELVPDDLSAWDADFSCGVPSQRIRLLALGAELSAL
                     AVDFTDVLNLVTPQAATPVNEDVYSLLTNGGRQDTPPHLALLFSFLNQFLNVQADLNS
                     LPKKHLDFFFQQVINLSPGTAIPDSAYVIFGLQKQVPTYIVKAGTLLKAGKDGTGADI
                     VFGVAQDTGFTQTQVASLRTVFVNQQEAGEQVYTEGVYMAPDATMADGISKPIKDPAG
                     AAWPALGAKQSLYVPPGAVVPIDYPLARLGFILASKVLLMKEGKRSVTIQLACQWRGL
                     CTGSVDFKTLFPQARDAVSSKYAVITADVLAKAATMGVTASTIGKLTDHYLTDPCRRD
                     PCDDTKKYQREQALVRIPADTSPDDFWTQEMKSIGGVAPWETEILEFVLVPQSLFTVS
                     FSGEKAWLTPDSLDMTLTAAGDASTMNFIWEIDAEIVTGRPAVAFYNAATLGENFNTT
                     DPLVKIQLNDTIKWELSEGSVAGVTSCLERRDPVCGQAMSAYEFFRNVVLVGAVSTNQ
                     VSEPTKITVMVCGVTSLVLQNDDNVLDGNKNFTPFGAKPAIVDFDVYPVKQPDGDNES
                     SSYGNLNLVGPSFLIGSAEVFYKKWDDVFINITWVGKPNDFNE"
     gene            complement(2363..2791)
                     /locus_tag="VKQ52_05850"
     CDS             complement(2363..2791)
                     /locus_tag="VKQ52_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HLZ86737.1"
                     /translation="MPSSNLNSFLGTGWKFPPTFDPELGSALMVSDEEDIQESLNILL
                     STALGERVMQPRYGCDLQPYLFDPLNANLIGFLKNRVYYAIIFYESRINLINITVSAD
                     SSTDSLEGKFRITVTYDITQTNTRFNYVYDYFQNEALQPI"
     gene            complement(2839..4578)
                     /gene="vgrG"
                     /locus_tag="VKQ52_05855"
     CDS             complement(2839..4578)
                     /gene="vgrG"
                     /locus_tag="VKQ52_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HLZ86738.1"
                     /translation="MPQELFIPNTSLHDVSSYQILINGQVANPVYELLSMVITREINR
                     LPAATIVFKDGDPSGKTFALSEQPDFVPGTPIAIKLGRDGTNDQAFKGIVVKHAIRVK
                     ENGQSELMVVCKDEVVRMTVGRHSKYYTKEKDSQLIDTLVTSYPNLSCNMDDSTLQYE
                     EVVQHHCSDWDFMLMRAEANGMLVSVIDGTVNINIPKTSATPVLTVAFGSSLLEFEAE
                     MDARNQWKNVEAHSWDYHNQALFTSDSSSVAGWSEAGNIAGSTLAQAINLANYELHHS
                     GYLTEAELKSWADGQLMRSRLAKICGRAKCNGFAGIKPGDMLTVSGVGARFNGNIFVT
                     AVRHEVGNGMWDTHIQFGLDSRRYAELYQDINDAQAAGLVSGIRGLQIGVTVKLEGDP
                     EGQDRIQVRLPMVDNNADGIWTRVASLDAGKNRGAFFRPEIGDEVIVGFINDDPREAV
                     VLGMLNSSAKPAPVTAQDKNDIKGFTTRSKMHITFDDGKNTITIDTPNKNQVLLDDTN
                     GKISISDQNQNSITMDSSGITITSATGDVNINATAGNVNLSAGLGFTAKGMTAALQGT
                     TTATVKATTVMIN"
     gene            complement(4578..5294)
                     /locus_tag="VKQ52_05860"
     CDS             complement(4578..5294)
                     /locus_tag="VKQ52_05860"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86739.1"
                     /translation="MSVSTSPSDSALSKMVIHSFSDKDFQQEEKDKLFTVPINPEAFT
                     KNYKVDLDNRTGHGQPGTQPGYKSSTPEELKIDFVLDGTGTVEGYPSQYDNVSVHDQL
                     TAFLDCVYTFNGSIHRPNFVIVIWGSEIAFPGTVSSVDLNHTLFLPNGTPLRIKVSVT
                     FVKSESDNARAAANKLQSPDLTYYHRVQRGDRLDLLTFSKYNDPNYFLQVGRINNLVT
                     VRKIAPPQELYFPPFAQNEP"
     gene            complement(5284..5460)
                     /locus_tag="VKQ52_05865"
     CDS             complement(5284..5460)
                     /locus_tag="VKQ52_05865"
                     /inference="COORDINATES: protein motif:HMM:NF039278.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HLZ86740.1"
                     /translation="MPIEVRELVIKATVEQETGSPGKPAPPPDKSNNTGANEALIQTC
                     IDKISQIIKTRYER"
     gene            complement(5463..5921)
                     /locus_tag="VKQ52_05870"
     CDS             complement(5463..5921)
                     /locus_tag="VKQ52_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HLZ86741.1"
                     /translation="MAGTTYQTVNFHFKVQFDFDTDGNSIDVSFQSVTGLDSTMETET
                     VKEGGENRFTHVLPVRRKFGPLVLKRGLLMPDDSMLTQYFKLAFENDVFTPFKLVTIH
                     LLDETHQSLMHWEVNNAWPLSWKIADLNAMEGAVLIETLELNYNRLIFNS"
     gene            complement(5948..6406)
                     /locus_tag="VKQ52_05875"
     CDS             complement(5948..6406)
                     /locus_tag="VKQ52_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HLZ86742.1"
                     /translation="MADPTKTYPLPKFHFQVSWGKTRMGFTEVTGLDFETEVIEYREG
                     NSVKYNKLKQPGLTKYSNITLKRGTFLGDFDYYELWKQTYFFQEVNGKYRTNVIIQLM
                     DEQHNPIITWSLANAWPTKIQATDLKADANEVAIESMELVHEGLTITEAP"
     gene            complement(6455..7420)
                     /locus_tag="VKQ52_05880"
     CDS             complement(6455..7420)
                     /locus_tag="VKQ52_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86743.1"
                     /translation="MINEFKYTEQREYDDSKILKCCPEPKPGQSDPPSSDCCYNNWQA
                     ELDVVNWELTEVTNELTHAQKHLALVTTRFTRLKTWNDELSSANELAFAVCRQLEIIE
                     AQLVNVCKNTQFTVKGVEILYCMVREFFLTLDQLQAKYDHLQNCIKCLNNAALQPGSG
                     IVKVLTDYGTALTAVLATRDALIVLVMSAMDSAVSLHRQICDGYGYKRLIRVWQDTLA
                     CGVPCEEGPVIKPLEAGKPQPEKAGVPGGPVTEPFCLEPVLHFPICNQAYFREIHALY
                     EEEKRDVKELTNEVKELTKRQLALQAIQQSLQNALKEVNPTVRCS"
     gene            complement(7451..8464)
                     /locus_tag="VKQ52_05885"
     CDS             complement(7451..8464)
                     /locus_tag="VKQ52_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86744.1"
                     /translation="MEQPKPSNVPDQGKKDKDRVKPEEKETATQKSIDAARQLYCTNL
                     RASKANQKAGEKAYERSVKIYRKKKRLFEWTENNYRIYRDLDICLDTELATGNAALTA
                     NVVNYNKVSAALYANLTQITAAVKTLKTKVSALRDQASVLENYKNDQCNAAQWAILTG
                     KNMENCKTDAPLPVPRERPEACKEADKIYEELIQIPKKLLVFDVDSLLQSSADVTGIQ
                     TFSNVAVLTKLQTDLTTASTALVTQIQAAVKARTADMATVQADLITSVQDCTKTGVDE
                     YNKISLCHAAHFSVQFLCQPDCGCVKPWQETAIEPRLEDCECRICRIGEVVRSVYRGG
                     PVE"
     gene            complement(8508..>9216)
                     /locus_tag="VKQ52_05890"
     CDS             complement(8508..>9216)
                     /locus_tag="VKQ52_05890"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HLZ86745.1"
                     /translation="QVKVALTTSTGTTNTTLDKIKGTSSQAYYAAENAINNNLDMLMP
                     AAPAMVGIYAAVDDSSGVWKAPANVAVLEAVDLEEQVSSFDQGQYLNIDPVAGLSINA
                     IRSFPGRGPAIVWGARTLAGNDNEWRYIPVRRFFFMAEQSIKNAIEPFVFEPNDNNTW
                     TRVTAMITNYLTELWKAGALMGSTTKDAFFVRVGIGVTMTAQDVLEGRMIVQVGMAAV
                     RPAEFIILQFLQMMPSA"
BASE COUNT         1909 a         2816 c         2339 g         2152 t
ORIGIN      
        1 tattcgttga aatcattggg cttcccgacc catgtgatat tgataaagac atcatcccat
       61 tttttataga acacctcggc gctgccgatc aggaagctgg gacccaccag gttcagattg
      121 ccataggaag agctctcgtt atcgccatcc ggctgcttca ccgggtagac atcgaagtcg
      181 acgattgccg gcttcgcccc gaacggcgta aaattcttat tcccatccag cacattgtca
      241 tcattctgca acacaaggct cgttaccccg cagaccataa ccgtgatctt tgtcggttcg
      301 ctcacctggt tcgtgctcac cgcacccacc agcaccacat tccggaagaa ctcatatgcc
      361 gacatcgcct gtccgcagac agggtcccgc cgttcgaggc aggaggttac gcccgccacc
      421 gaaccttcgg aaagttccca cttgatcgtg tcattcaact gtatcttcac cagcggatcg
      481 gtggtattga aattctctcc cagcgtggcg gcattgtaaa aagcaaccgc cggccgtccc
      541 gtcacgatct ccgcgtcgat ctcccagatg aaattcatcg tcgacgcatc tcccgcggcc
      601 gtaagcgtca tatccaggct atccggcgtc agccaggcct tctccccgct aaagctcacc
      661 gtaaaaagcg actgcggcac aaggacgaat tccaggatct ccgtctccca gggcgccacc
      721 ccgccgatgg acttcatctc ctgcgtccag aaatcgtccg gcgaagtatc ggcgggtatc
      781 cgcacaaggg cctgctcccg ctggtatttc ttcgtatcgt cacaggggtc ccgccggcag
      841 gggtccgtaa ggtaatgatc ggtcagttta ccgatcgtcg aagccgtaac gcccatcgtc
      901 gccgcttttg ccagcacatc cgccgtgatc accgcatatt tcgagctcac cgcatcccgg
      961 gcctgcggga acagggtctt gaaatccact gatccggtgc aaagcccccg ccactgacag
     1021 gccagttgaa tggtgacact ccgcttgcct tccttcatca gcaatacctt cgaggccagg
     1081 ataaacccaa gccgtgcaag cgggtaatcg atagggacaa ccgcccccgg cggaacatat
     1141 aaactctgct tcgccccaag cgccggccac gcggcgccgg ccgggtcctt aatgggtttg
     1201 gagatcccat cggccatcgt cgcatcgggg gccatataaa cgccttcggt atacacctgc
     1261 tcacccgcct cctgctggtt gacgaacacg gtgcgcaggc tggctacctg tgtctgcgta
     1321 aaccccgtat cctgtgctac tccaaaaaca atatccgccc ccgtgccgtc ctttcccgct
     1381 ttcaacagcg ttcctgcctt cacgatatag gtcggcacct gcttctgcaa tccgaaaatg
     1441 acataggcgc tgtccgggat cgccgtcccc ggcgacaaat ttatcacctg ctgaaagaag
     1501 aaatcgagat gtttcttcgg cagactgttc aaatcggcct gtacattcag gaattgattc
     1561 agaaaagaga acaacaacgc caggtggggc ggggtgtcct gccgtccgcc gttcgtcagc
     1621 agggaataca catcctcatt gacgggggtg gccgcctgtg gcgtcacgag gttcagcaca
     1681 tcggtaaaat ccaccgccag ggcactcagc tccgccccca gcgcaagcaa ccggatccgc
     1741 tggctcggca cgccacagga aaaatccgca tcccaggccg acagatcgtc cggcaccagc
     1801 tcccaagccg cgttcgcctg taactctacc gccgccggcg cctggatacc gaagcaatgc
     1861 gccgcggtat tcgcatggct gatatattgc ttgatggagg ggacaagccg gtcgcggatc
     1921 agcgtttgca acccctgctc cagggcgagt ccgctgcccg caagacccag cgaccattgc
     1981 tgcaagggat agatcgcggt ataataggtt tgatagaaca acaattgaag accatcgggt
     2041 gtcggctgtt tccggaacaa ccggaccaca tcatccatcc gggcctttac cgaatccccc
     2101 gaatagctcg aaatgctgct gagcaaaaag ggcaggctgt tgctgaagaa aggcttccac
     2161 gacccaacac taagatccga attgtaaaaa ttgatctgcc ctgccagcgc cgtaaaatag
     2221 ttcagcagat cggatagctg ccgcccgtcg atcggtgtcg tatccccaag cagatcggcc
     2281 ggagcccggt ccccctggct ggtgccgggg tcattctgaa aaggatgccg taaatgatcg
     2341 caactgaaca tgaagagtca atttagatag gctgcaatgc ttcgttctgg aaatagtcgt
     2401 acacatagtt gaaccgcgta ttcgtctgcg tgatatcata ggtcaccgtg atccggaact
     2461 tcccctccag gctgtccgtc gatgagtcgg cgctgacagt gatattgatc aggttgatcc
     2521 tggattcata aaagatgatc gcataataca cccggttctt gagaaagccg atcaggtttg
     2581 cattcagcgg atcgaaaaga tagggctgaa gatcacagcc atacctgggc tgcatcaccc
     2641 gctcccccag cgccgtcgac aacaggatat tcaggctctc ctggatatcc tcttcatcgc
     2701 tcaccatcag ggcgctcccg agttcgggat cgaaggtcgg cggaaacttc cagccggtgc
     2761 caaggaagga attgagattc gaagaaggca tagattgaat aattttttat tgcgtgccct
     2821 gtcaggagcc cgtataggtt aattgatcat tacggtggtg gccttgacgg ttgccgtcgt
     2881 agtcccctgc agggctgccg tcattccctt cgcggtaaac ccaagccccg cactcaggtt
     2941 gacattcccc gccgtcgcgt tgatattgac atctcccgtc gcactggtga tcgtgatccc
     3001 cgacgaatcc atcgtgatgg agttctggtt ctgatcgctg atactgatct tcccgttcgt
     3061 atcatcgagc aggacctggt tcttgtttgg cgtatcgatc gtgatcgtat tcttcccgtc
     3121 gtcgaacgtg atatgcatct tgctccgggt ggtgaaccct tttatatcgt tcttgtcctg
     3181 ggccgtgacc ggcgccggct tggcgctgct gttcagcatt cccagtacaa ccgcctcccg
     3241 cggatcgtca ttgatgaacc ctacgatcac ctcgtcgccg atctccggcc ggaaaaacgc
     3301 cccacggttc ttcccggcat ccagcgaagc cacccttgtc cagatgccat cggcgttatt
     3361 gtccaccatc ggcagccgca cctgtatcct gtcctgcccc tcgggatcac cctccagttt
     3421 gaccgtgacc ccgatctgca gcccgcggat gccgctgacc agccccgccg cctgcgcgtc
     3481 gttgatatcc tggtacagtt ctgcatatcg ccgcgagtcg agcccgaact ggatatgcgt
     3541 atcccacatc ccattcccca cctcgtgccg tacggccgtg acaaagatat tcccgttgaa
     3601 ccgggcccca accccgctga cagtgagcat atcccccggc ttgatcccgg caaacccatt
     3661 gcacttggcc cgcccgcaga tcttggccag ccggcttcgc atcaattggc cgtccgccca
     3721 ggatttcagt tccgcctccg tgagatagcc gctatgatga agctcgtaat tggccaggtt
     3781 gatggcctgg gcaagcgtgc tgcccgcgat gttccccgcc tccgaccatc ccgccacact
     3841 cgacgaatcc gacgtgaaca gggcctggtt gtgataatcc caggaatgcg cctcgacatt
     3901 cttccactgg ttacgggcat ccatctccgc ctcgaactcc agcagcgatg atccaaaagc
     3961 caccgtcaac acaggggtag cgcttgtctt cgggatattg atattcaccg tcccgtcgat
     4021 gacactcacc agcatcccgt tggcttcggc ccgcatcagc ataaaatccc agtcgctgca
     4081 atgatgctga accacctcct cgtactgcag cgtgctgtca tccatattgc agctgaggtt
     4141 gggataggag gtcaccagcg tatcgatcag ttggctgtcc ttctccttgg tgtagtattt
     4201 actatgccgc cctaccgtca tccgcacgac ctcgtccttg cacaccacca tcagttcact
     4261 ctgcccgttc tccttcaccc ggatggcatg cttcacgaca atacccttaa aggcctggtc
     4321 attcgttccg tcccgcccca gtttgatcgc gatgggcgtc cccggcacaa aatccggctg
     4381 ctcgctaagc gcaaacgtct tcccggaagg atcgccatcc ttgaagacga tcgtagcggc
     4441 aggcaaccgg ttgatctcgc gggtgatcac catcgacaat aactcgtaga caggattcgc
     4501 cacctgcccg tttataagta tctggtaact cgagacatcg tgcaacgacg tattgggtat
     4561 gaataattcc tgcggcatca gggttcattt tgtgcgaatg gtggaaaata aagctcctgc
     4621 ggcggggcga tctttcgcac cgtgactaaa ttgtttatac gccccacctg caggaagtaa
     4681 ttgggatcat tatattttga aaaggttagt aagtccagcc tgtccccccg ctgtacccgg
     4741 tggtaatagg tcagatcggg cgactgcagc ttattggcgg ctgcccgcgc attgtccgac
     4801 tcggatttga caaaggtgac cgataccttg atccgcaacg gcgttccgtt cggcaggaaa
     4861 agggtatggt tcaggtccac actcgacacc gtaccgggaa acgcgatctc cgaaccccag
     4921 atcacgataa caaaattcgg ccggtgaatg cttccgttga aggtatagac acaatccaga
     4981 aaagccgtca gctgatcgtg caccgacaca ttatcatact gcgatggata tccctccacc
     5041 gtacccgtcc cgtccagcac aaaatcgatc ttcagttcct ccggggtgct cgacttgtaa
     5101 cccggttggg tcccgggctg accatgcccc gtccggttgt ccaggtctac cttatagttc
     5161 ttggtaaacg cctcggggtt gatcggcacg gtgaacagct tatccttttc ctcctgctga
     5221 aaatccttgt cggaaaagga atggatcacc atcttggaca gggcgctgtc gctgggagag
     5281 gtgctaacgc tcatagcgtg tctttatgat ttgggaaatt ttatcgatac aggtctgtat
     5341 cagcgcctca ttggcgccgg tgttattact cttgtcgggc ggcggagcag gcttccccgg
     5401 cgaaccggtc tcctgttcga ccgtggcctt gataaccagc tcccgtactt cgataggcat
     5461 aactaggaat taaagattag tcggttataa ttcaattcga gcgtctcgat caggaccgcg
     5521 ccctccatgg cattcagatc ggcgatcttc cagcttagcg gccaggcatt gttgacctcc
     5581 caatgcatga ggctctgatg cgtctcgtca agcaggtgta tcgtcaccag cttgaatggc
     5641 gtgaatacat cattctcgaa agccagtttg aaatactgcg tcagcatcga atcatccggc
     5701 atcagcagac cccgcttcaa taccaggggc ccaaatttgc ggcgcaccgg caaaacatgc
     5761 gtgaaccggt tctctccacc ctccttcacc gtctccgtct ccatcgtgga atcgagcccg
     5821 gtcaccgact ggaagctgac atcgatgctg ttgccgtccg tatcgaaatc gaactgcacc
     5881 ttgaaatgaa aattcacggt ctgataggtc gtccccgcca tagcctatgg ttttatggcc
     5941 ctctttgtta tggagcctcc gttatggtta aaccttcgtg taccagctcc atcgactcga
     6001 tcgccacctc gttggcatcc gccttcaggt ccgtcgcctg gatctttgtc ggccaggcat
     6061 tggccaggct ccacgtgatg atcgggttat gctgctcgtc catcagctgg atgatcacat
     6121 tcgtccggta tttgccattc acttcctgga agaaataggt ctgcttccac agctcgtaat
     6181 aatcgaaatc gccaaggaag gtgccgcgct tcagcgtgat attgctgtac ttggtgagcc
     6241 ccggctgctt cagcttgtta tacttcacgc tgttgccttc ccggtattcg atcacctccg
     6301 tctcgaaatc gaggccggtg acctccgtaa aacccatccg tgtcttgccc caggatacct
     6361 ggaaatggaa tttcggaagg ggatatgttt ttgtaggatc tgccattgtt cttggtttta
     6421 aaactgatta attggttatc gcgtgaataa aagtctagct gcaacgcacc gttgggttca
     6481 cttccttcaa ggcgttctgc agactctgct ggatggcctg cagcgccagc tgccgcttgg
     6541 taagttcctt cacctcgttt gtcagctcct tcacgtcccg cttctcctcc tcgtacaagg
     6601 catggatctc cctgaaatag gcctggttgc agatcgggaa atgcaacacc ggctccaggc
     6661 agaaaggctc cgtcaccggc cctcccggca ccccggcctt ttccggttga ggtttccctg
     6721 cctccagcgg tttgatcacc ggcccctcct cgcaggggac cccgcaggcc agcgtgtcct
     6781 gccagacgcg gatcagccgc ttgtaaccat agccatcaca gatctgccgg tgcaggctga
     6841 cagccgaatc catggcgctc atcaccagca cgatcagcgc gtcccgcgtc gccagcaccg
     6901 ccgtcagcgc cgtgccatag tccgtcagca ccttgacgat acccgaaccc ggctgcagcg
     6961 cggcattgtt cagacacttg atacagtttt gtaaatggtc gtatttcgcc tgcagctggt
     7021 ccagggtcag aaagaactcc cgcaccatgc aatagaggat ctcgactccc ttaacggtaa
     7081 actgcgtatt cttacagaca ttcaccagct gtgcctcgat gatctccagt tgccggcaga
     7141 cggcaaaggc cagctcattg gcggaagaga gctcgtcatt ccaggtcttt aaccgggtga
     7201 aacgcgtcgt caccagcgcc aggtgcttct gcgcatgcgt cagctcattg gtcacctccg
     7261 tcagctccca gttcaccaca tccagctcgg cctgccagtt gttataacaa caatcggaac
     7321 tgggaggatc ggactggccg ggctttggct cgggacaaca tttgaggatt ttggaatcat
     7381 cgtattcccg ctgttcggta tacttgaatt cattgatcat aagaagatat ttaagtaata
     7441 acaaataaga ttattccacc ggccctccgc gatagacgct tcgcactact tcaccgatgc
     7501 ggcagatgcg gcattcacag tcctccagcc gcggctcgat cgccgtctcc tgccagggtt
     7561 tgacacaacc gcaatccggc tggcaaagga actgtaccga gaaatgggcc gcatgacaga
     7621 ggctgatctt gttgtactcg tccacgcccg tcttcgtaca atcctgcacc gaggtgataa
     7681 gatcggcctg cacggtggcc atatcggccg tccttgcctt caccgcggct tgtatctgcg
     7741 tgacgagcgc cgtcgaagcg gttgtaagat cggtctgcag cttggtcaat acggcgacat
     7801 tcgaaaaggt ctggatgccc gtgacatcgg ccgaagactg cagcagcgag tcgacatcga
     7861 agacaagcag cttcttgggg atctggatca gttcctcata gatcttgtcc gcttccttgc
     7921 aggcttcggg ccgctcccgg ggcaccggca gcggtgcatc cgtcttgcag ttctccatat
     7981 tcttcccggt caatatcgcc cactgggcgg cattgcactg gtcgttctta taattctcca
     8041 gcacggaggc ctggtcccgc agcgcggaga cctttgtctt caacgttttg acggcggcgg
     8101 ttatctgcgt gagattcgca tacaacgcgg ccgacacctt attataattg accacattcg
     8161 ccgtcagcgc tgcattcccg gtggccagct ccgtgtcgag acagatatcc aggtcacgat
     8221 agatccggta gttgttctcc gtccactcga aaagccgttt tttcttccgg tagatcttta
     8281 cacttcgctc gtaggctttt tctccggcct tttgattcgc cttggaagcg cggagattgg
     8341 tgcaatacaa ctgccgggcg gcatcgatgc ttttctgggt ggccgtctcc ttttcttctg
     8401 gctttacgcg gtccttgtcc tttttgccct ggtcgggaac attggaaggc tttggttgct
     8461 ccatgattgt aagtttaaaa atttaaataa tgtaggttgt cgctgtctta ggcggaaggc
     8521 atcatctgca ggaattgcag gatgataaat tcggcgggcc ttaccgcggc catccccacc
     8581 tgcacgatca tccggccctc gaggacatcc tgcgccgtca tcgtcacgcc gatccccact
     8641 cgtacaaaaa aggcatcctt ggtcgtactt cccatcagcg cgccggcctt ccagagctcc
     8701 gtaagatagt tcgtgatcat cgccgtcacc ctcgtccagg tgttgttgtc attcggctcg
     8761 aagacaaacg gctcgatcgc attcttgatg gactgctcgg ccataaagaa gaagcggcgc
     8821 accgggatat accgccactc gttatcattg ccggcaagcg tccgcgcacc ccagacgatc
     8881 gccgggcccc tgccgggaaa actgcggatc gcattgatgg acaagcccgc taccgggtcg
     8941 atattcagat actgcccctg atcgaagctg ctcacctgct cctccaggtc cacggcttcc
     9001 agcaccgcga cattggccgg cgccttccat accccgctgc tatcatcgac agcggcatag
     9061 atgcccacca tcgcgggggc cgccggcatc agcatatcga gattattgtt gatggcattc
     9121 tccgccgcat aataggcctg gctgctggtg ccctttatct tgtcaagcgt ggtgttggtc
     9181 gttcccgtcg aggtggtcag cgccactttc acctgc
//
LOCUS       DATGFG010000314         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_373329, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000314 DATGFG010000000
VERSION     DATGFG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373329"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2158)
                     /locus_tag="VKQ52_05895"
     CDS             complement(<1..>2158)
                     /locus_tag="VKQ52_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HLZ86746.1"
                     /translation="TEAGVSRFDGTHFRNFTTVDGLPDVEVLEMFADSRGRVWMAPFR
                     KSLCYFYKGKICNQENDPLLHNIRLRNTIHGFAEDRAGDVLAATRTALFLIKPDGRVR
                     EIDSLNGRPLQNCGAIATGNDGNFLVQSANEVYRLSGEKFTPLYAISIQDSWPFYISM
                     NSAGMIWRRDSVSAAVHSFSTGKTVYLPFERVHYRHNTFTSLGDSLFCFNENNGVNEY
                     NLFTGQFRRMLPGKQVSRSFRDAAGNTWFTTIGQGIYRLNSDEFRSIRLSTPHSVSSS
                     IQAIRRIGDMLLVGGDLNYIFVFRFPAMSLNKTVAFSGGIKNRITYFGTASNGNVYVV
                     TDGGIRWLSRDFRTFGAAFTGAKACLPFGSGKLLIATYWGVGIFDLHSFKILDTIFRE
                     RTTALYYHRDTILVGTLEGLYGIAPDRSVHFLGKSIPFLRRRISALTESADGTLWVAS
                     YDAGIIGYKDGAVIARFGKQQGLSSDICRTVLIHNNALWVGTDKGLTKVSLNRPGYPM
                     EHYTSQDGLGSDIINTIYADGPVIYVGTPVGFSMFDETRIDVSESCRLYLLSIQNGDR
                     ERIGDSASLLLPYMDKHLRLEFAGISYRSVGDITYQYRLVGLDSAWRTTRESFLEYPT
                     LPSGKYQLQLQAVNKFGVQSNIVTLPFEVETPWWQTTLFYILQVAVFLSITWLGVSLR
                     IRQIRNRQREKEKLNQRMMKMEHMALQAQMNPHFIFN"
BASE COUNT          544 a          573 c          547 g          494 t
ORIGIN      
        1 gttgaagatg aaatgcggat tcatctgggc ctgcagcgcc atatgttcca ttttcatcat
       61 ccgctgatta agcttttcct tttcccgctg ccggttgcgg atctgccgga tccgcaggga
      121 aacgcccagc caggtgatag acaaaaaaac cgctacttgc aagatataga acagcgtcgt
      181 ctgccaccac ggcgtttcca cttcgaaggg taaggtaacg atattgcttt gtaccccgaa
      241 tttgttgacc gcctgcaatt gcagttggta tttgcccgaa ggaagggtag gatattcgag
      301 gaaggactcc cgggttgttc gccaggcgct gtccaggcca accaaccggt attgataggt
      361 aatatcaccc accgacctgt aagaaatgcc ggcaaactcc agccggaggt gcttgtccat
      421 ataagggagc aacagggacg cggagtcccc gatccgctcc cggtccccat tctggatgga
      481 taacagataa agtcggcagc tttccgaaac atctatcctg gtctcatcga acatactgaa
      541 tcccacaggc gtacctacat aaatcacagg tccgtcagca taaatagtat tgatgatatc
      601 ggacccaagg ccgtcctggg aagtatagtg ttccatcgga taacccggcc tgttcaggct
      661 gacctttgtc aatcccttgt cggtgccgac ccaaagtgca ttgttgtgaa tcaggacagt
      721 tcggcaaatg tcgctgctca gtccctgttg tttgccgaag cgggcgatca ccgctccatc
      781 cttgtacccg atgatacctg cgtcgtatga ggcgacccat agggttccat cggcagattc
      841 agtcagggcg gatatccttc ttcggaggaa gggaatcgat ttgcccaaaa aatgaacaga
      901 tctgtcaggc gcgataccat agagtccctc cagcgttccg accaatatag tatcgcgatg
      961 gtaatataag gcagtagtgc gttcccgaaa gatcgtgtcc aggatcttga aactatgaag
     1021 atcgaaaata cccacccccc aatacgtggc gatcagcaat ttgccagagc caaatggcag
     1081 acacgctttc gcgccggtga atgccgcgcc aaaagtgcgg aagtcacggg ataaccaccg
     1141 gatacccccg tcggtgacaa catacacatt gccattggaa gcagtgccga aataggtgat
     1201 gcgatttttt atcccgccgc tgaacgcaac agtcttgttg agggacatag ccggaaaccg
     1261 gaaaacaaaa atgtaattca ggtctcctcc caccaggagc atgtcgccga tccttctgat
     1321 cgcctgaatg gacgaactga cagaatgggg tgtgcttaac ctgatggatc gaaactcatc
     1381 tgaattaagc cggtatatgc cctgaccgat cgttgtaaac catgtgttac ccgcggcatc
     1441 cctgaaactt cgcgatacct gtttgcccgg caacatgcgt ctgaactgtc ccgtaaagag
     1501 gttatactca ttgacgccgt tattttcatt aaaacaaaac aagctgtccc cgagactcgt
     1561 aaaggtatta tgacggtagt gaacccgttc gaacgggaga tatacggtct tgcctgtgga
     1621 aaaagagtga acagccgctg aaacactatc cctccgccag atcatgccgg cgctgttcat
     1681 ggaaatatag aagggccaac tgtcttgaat gcttatcgca tatagcggtg taaatttctc
     1741 acccgacaat ctgtatacct cgttcgccga ctggacaaga aaattcccat cgttaccggt
     1801 ggctattgct ccacagttct ggaggggccg gccgttgagg gaatctattt cacggaccct
     1861 gccatccggc tttataagga aaagtgctgt cctggtcgcg gccaggacat cgcccgcgcg
     1921 gtcttccgca aagccatgga tggtattccg cagcctgata ttatgcagaa gggggtcatt
     1981 ttcctggttg cagatctttc ccttgtaaaa gtaacagagg gatttccgga aaggcgccat
     2041 ccagacccgg ccccgggaat ccgcgaacat ttcgaggacc tcgacatccg gcaggccgtc
     2101 caccgtagtg aaattgcgga aatgggtgcc gtcgaaacga ctaaccccgg cttcggta
//
LOCUS       DATGFG010000315         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_488965, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000315 DATGFG010000000
VERSION     DATGFG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488965"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VKQ52_05900"
     CDS             <1..1168
                     /locus_tag="VKQ52_05900"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLZ86747.1"
                     /translation="RHHPKKPGTMNNEEDLRKWRLILGGQQNDGTGFTLDGQDLSVDK
                     TLEALYDSKKQGGLGPSSPNVSRWLGDIRTFFPNSVVQVMQQDALKRLNLTQMLFEKE
                     TLENIAPDVHLVATLMTLSKVIPAKTRDTARQVVRKVVDDLMKKLAQPTQQAITGSLK
                     RSTRNRRPRHNEINWHSTILKNLKHYQPEYKTIIPETRIGYGRKRQSLKDIVLCIDQS
                     GSMGTSVVYSGIFGAVMASIPAVRTKMVVFDTAVVDLTEELSDPVELLFGVQLGGGTD
                     IHLAIGYCQQIITRPLDTVMVLVTDLYEGGNQEGMRKRATDLVGAGVQLIVLLALSDD
                     GAPAYDHANAQFLSNLGVPVFACTPDLFPSLMAAALSKQDVAQWAAKEDLVLKK"
     gene            complement(1379..1594)
                     /locus_tag="VKQ52_05905"
     CDS             complement(1379..1594)
                     /locus_tag="VKQ52_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86748.1"
                     /translation="MTQAKLVHLDKDNADFSKLAIVDLEIIPRIGESVYYAKSSDDDP
                     VGYTVDNVVHSIQSERPLEIWLVTPQK"
     gene            2195..>2941
                     /locus_tag="VKQ52_05910"
     CDS             2195..>2941
                     /locus_tag="VKQ52_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86749.1"
                     /translation="MPQNIHLSIDNPCAEHWDQMTPDQQGRFCASCQKTVVDFAQMND
                     QEVLNWLGRHQGPTCGRFRQDQLQRPLVATPEKKPGPGRYWHYLIALLLSSSEIAAQT
                     QPAKPATSQQTPSNGEGRLAIANTLIGDTILSPARMLPAGTFRGRLLDVDGNPVSFAT
                     IMYREKQGVAADAEGYFSIPIANLAGVHILKITAVGYQSTQLDISKIPPEGKILIFPI
                     VRMETVVVGELVTVTRRKRKRPVADTLSMIK"
BASE COUNT          744 a          924 c          688 g          585 t
ORIGIN      
        1 tcggcatcac cctaaaaaac ccggaacaat gaacaacgaa gaggatttga gaaaatggcg
       61 gctgatcctt ggcggtcagc agaatgacgg caccggcttc accctggacg gccaggacct
      121 gagcgtggac aaaacactcg aggcgctgta cgacagcaag aagcagggcg gtctcgggcc
      181 atcctccccc aacgttagcc gctggctcgg cgatatccgc accttcttcc ccaacagtgt
      241 ggtgcaggtc atgcaacagg acgccctcaa gcgactgaac ctcacgcaaa tgcttttcga
      301 aaaggagacg ctggaaaaca ttgcccccga tgtgcacctg gtggccaccc tcatgaccct
      361 gagcaaggtc attcccgcga agaccaggga caccgcacgc caggtcgtcc gcaaggtggt
      421 tgacgatctc atgaagaagc tggcccaacc cacccagcag gccatcacgg gcagcctcaa
      481 acgcagtacc cgcaatcgca ggccgcgtca caacgagatc aactggcatt cgacgatcct
      541 caagaacctc aagcactacc agcccgaata caagaccatt attcccgaaa cccggatcgg
      601 ctatggccgg aagcggcagt cgctgaagga catcgtcctc tgcatcgacc agagcgggtc
      661 gatgggcacc tccgtggttt acagcgggat cttcggggcg gtgatggcgt ctatccccgc
      721 ggtccgtacg aagatggtgg tcttcgacac ggccgtcgtc gacctcaccg aggagctcag
      781 cgatccggtc gaactcttat tcggcgtcca actgggcggc ggcaccgata tccacctggc
      841 catcggctac tgccagcaga tcattacgcg gcccctcgac acggtcatgg tcctcgtaac
      901 ggacctctat gaaggcggca accaggaagg catgcgcaag cgcgccaccg acctagtggg
      961 tgccggcgtc caactcatcg tcctcctcgc cctctccgac gacggggcgc ccgcctacga
     1021 ccacgccaac gcccaattcc tctcgaacct cggcgtccct gtcttcgcgt gtacccccga
     1081 tctcttccct tccctcatgg ccgccgccct aagcaagcaa gacgtcgctc aatgggccgc
     1141 aaaagaagac ctggtgctga aaaagtagga tgcctatttc cgatttgggc gttccccctt
     1201 cggggtcagg ctctccgctg caagtcctcg ctacgctccg ggctttccgc tccgatcctt
     1261 aacgtccgac ccgttaggac ctgtgtatgc ccccgaaata cctccatcag gatacagagc
     1321 atttatacag cctttaagca gaaaatagga cgcaaattcc tgaaatgtta caccgtcttt
     1381 acttctgcgg cgttaccagc cagatctcaa gcggccgttc tgattgtatg gaatgcacta
     1441 cattgtcgac agtataccct acgggatcgt cgtcgctgct ttttgcgtag tagactgact
     1501 cgccaattct tggaataatt tcaagatcta caattgccag tttgctgaaa tcagcattgt
     1561 ctttgtccag gtgaactaac tttgcttgtg tcatagataa agtttatttg tagtgagttg
     1621 aagttaagaa aaaacaaaaa gcgccgctca taatggtaca aaatttccgt taaacccgct
     1681 gcctaaggtt cattaccaac ccagcgccac cttcccaaac aagctcaacc cattaaaccc
     1741 catttgcacc gaatcaagga ttgacttccc ccattcaaat atcacgtcgc caaatgggcc
     1801 gccaaagcgc ggttcatgtt gaatatgtag agagccatta gacggaaaaa aagggagaac
     1861 tttcccaggc ggcaactatt cggaaattcc gaacagttca aattgagctc aaaccatatt
     1921 cctgctttaa agaattacct agcaaaagtt gaccagaatg cagttcccac gtttcgtaaa
     1981 cgacccttgt aacatacaac atcctttccc gaccccagca gtttcagata ataaataaac
     2041 ggcatctggt gggtcactac cctcgagaac gccgaaggaa gcgaaatcgc aaaacccttc
     2101 aacccgacca ccctcctcgg ccacaaacaa aaataaaaac ccctatttta tgtaatccca
     2161 cccaaacccg catcctattg aaaacagacc gcacatgccc caaaatatcc acctctctat
     2221 cgacaacccc tgcgccgagc attgggatca gatgaccccc gatcagcagg gccgcttttg
     2281 cgcctcctgc caaaagacag tcgtcgattt tgcccaaatg aacgatcagg aagtattgaa
     2341 ctggctcggc cggcaccagg gacccacctg cggccggttc cgccaggacc aactccaacg
     2401 gcccctcgtc gccacaccgg aaaaaaagcc cggcccgggc cgctactggc attatctgat
     2461 cgccctgctc ctctcttcct ccgaaatagc cgcacaaacg cagccggcga aaccggcaac
     2521 cagtcagcag acgccgtcca acggcgaagg ccggctcgcc atcgccaata ccctcatcgg
     2581 cgatacgatc ctttctcctg ctcgaatgct acccgccggc acctttcgcg gtcgcctact
     2641 ggacgttgac ggaaaccccg tctcctttgc gacaattatg tacagggaaa agcagggcgt
     2701 cgcggccgat gccgagggct atttttctat cccgatcgcc aatctggccg gcgtccacat
     2761 cctaaagatc actgctgtgg gctatcagtc tacccaattg gatataagca aaatcccacc
     2821 tgaagggaaa attctgatct tcccaattgt acgaatggaa accgtagtgg tgggtgagct
     2881 agtcacggtt acccggcgca aaaggaaaag accggtcgca gacaccctct cgatgataaa
     2941 a
//
LOCUS       DATGFG010000316         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_740954, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000316 DATGFG010000000
VERSION     DATGFG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740954"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..841)
                     /locus_tag="VKQ52_05915"
     CDS             complement(314..841)
                     /locus_tag="VKQ52_05915"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86750.1"
                     /translation="MSERIIIDMDEVIADPMGEMTEWYKKEYGAEPDKSKMIGSWVKG
                     FPEEHHQLIMDRLKSPGFFRHLPVMADSIEVLRELNRRYEVFIVSAAMEFPNSLKDKY
                     DWLMDHFPFLTWRQIALTGSKDLVFGDFMIDDHVKNLKGFKGKPYLFTSQHNLTVTGY
                     DRINDWKEAADIFLK"
     gene            complement(838..>3241)
                     /gene="uvrA"
                     /locus_tag="VKQ52_05920"
     CDS             complement(838..>3241)
                     /gene="uvrA"
                     /locus_tag="VKQ52_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HLZ86751.1"
                     /translation="KKFRNKKISVLAPLVRGRKGHYRELFEDIRKKGYLKVRVDGEVK
                     DLAPKMQVDRYKIHDIEVVIDRLAVTEDMKPRLSQSVQKALQMGKDLLFLLLNDDNKV
                     FQYSKQLMCEDTGISYEEPSPNSFSFNSPYGACPTCKGLGVVYQISLDAIIPDPSKSI
                     NDGGIVPLGGEREAYMFRQVQGLAKKGRFSLDTPIKDMPKKALNLVLYGNEAGDTTLD
                     VEFDENASVGQIYTADYEGIIPALKRWFSGGTTDALREWVERFMELKSCGTCGGTRLR
                     KESLWFRVGEKNIAELSAMNLNRLIEWFDGVEKKLNNKQNIIAKDILKEIRERLQFLS
                     DVGLTYLTLDRSSRTLSGGESQRIRLATQIGSQLQGITYILDEPSIGLHQRDNHQLIQ
                     ALKNLRDIGNSVLVVEHDKDIMLAADYLVDIGPKAGFHGGRVVAQGRPHALLKLNTLT
                     AEYLNGHRKIEVPTHRRKGNGKFLELKGARGNNLKNVDAKLPLGELIVVTGVSGSGKS
                     TLINETLYPILSKHAYGSRVTPLEYKSIKGLENIDKVIEIDQSPIGRTPRSNPATYCG
                     FFTDIRTLFASVPEAKIRGYNAGRFSFNVKSGRCDVCEGGGMRVIEMNFLPDVYVHCE
                     KCNGRRYNRETLEIRYKGKSISDVLDMTVDEAVEFFQAVPYLYRKIKVLQEVGLGYIT
                     LGQSAVTLSGGEAQRVKLGTELGKKDTGKTFYILDEPTTGLHFEDIRHLLEVLNKLVD
                     RGNTVLVIEHNLDVIKVGDHLIDLGPEGGDGGGRILFEGTPEELIKNKESHTAKFLKD
                     EL"
BASE COUNT          626 a          864 c          923 g          828 t
ORIGIN      
        1 atccgcaacc gtgagaccac ggtgcttaca aatatttccg aaggaaactt tcttttcgag
       61 gttttcaccg atgacgagcg tattgaaaac gggcaattga cggtgaggtg atctcagcaa
      121 tgtctaacca ataagcaagc agggaaattg gtaaaatccg aatatccgaa ggttaagtaa
      181 aacctgatcc gttccctatt taggtaccct ttacaaacac aaagatttta ttaatcactc
      241 cctgctaccg cttattggga gtttaaagac aaaactggcc gcccgcgcgg ccatttatca
      301 tttgcggcca ttgtcatttc aggaagatgt ccgcggcttc tttccagtcg ttgatccggt
      361 cgtagccggt gacggtgagg ttgtgctggg aagtaaagag atagggtttc cccttgaatc
      421 cctttagatt ctttacatga tcgtcgatca tgaaatcgcc gaagacgagg tccttactcc
      481 cggtgagggc tatctgcctc caggtaagga aggggaagtg atccatcagc cagtcatatt
      541 tgtccttcag cgaattgggg aattccatcg cggcggagac gatgaacacc tcgtagcggc
      601 ggttcagttc ccgcaggacc tcgatgctgt ccgccatgac gggcaggtgg cggaagaagc
      661 ccggcgactt cagcctgtcc atgatgagct ggtggtgttc ttcggggaag ccttttaccc
      721 aggagccgat cattttgctt ttatcgggtt cggcgccata ttccttttta taccattctg
      781 tcatttctcc catcgggtcc gcgatgacct cgtccatgtc gatgatgatg cgttcgctca
      841 taattcgtcc tttagaaatt ttgccgtatg actttctttg ttctttatca gttcttcggg
      901 ggtgccttcg aagaggatcc tgccaccgcc gtcgccgccc tcgggtccca ggtcgatgag
      961 atggtcgccg accttgatga catcgaggtt gtgttcgatg acgagcacgg tattcccgcg
     1021 atcgacgagc ttgttgagca cctcgagcag atggcggatg tcctcgaagt gaagcccggt
     1081 ggtgggttcg tcgaggatat aaaaagtctt ccccgtgtcc tttttgccta attccgtacc
     1141 cagtttcacg cgctgggcct cgccgccgct gagggtaacg gccgactgac cgagcgtgat
     1201 atagcccaga cccacctcct gtaacacttt tattttccgg tacagatagg gcaccgcctg
     1261 gaagaactcg acggcttcgt cgacggtcat gtcgaggacg tcgctgatgg atttgccttt
     1321 atagcggatc tccagcgtct cccggttgta gcgcctgccg ttgcatttct cacaatggac
     1381 atagacatcg ggcaggaaat tcatctcgat gacgcgcatg ccgccgcctt cgcagacgtc
     1441 gcagcggccg cttttgacgt tgaaggagaa ccggcccgca ttataacccc tgatcttcgc
     1501 ttcgggtacc gaggcaaaaa gcgtacggat atccgtgaag aacccacagt aggtggcggg
     1561 gttgctccgg ggagtacggc cgatcggcga ctgatcgatc tcgatcacct tgtcgatgtt
     1621 ctcgagaccc ttgatgctct tgtattccaa tggcgtcacc cggcttccgt aagcatgttt
     1681 gctgaggatg ggatagaggg tctcgttgat cagggtggat ttcccgctgc cgctcacccc
     1741 ggtgacgacg atcagttctc ccaggggcaa tttggcatcg acattcttca ggttattgcc
     1801 cctggcgccc ttcagctcga ggaatttacc gttgcccttc cggcgatggg tggggacttc
     1861 tattttccgg tggccgttga ggtattcggc cgtcagggta ttgagtttca gcagggcatg
     1921 cgggcggccc tgggcgacga cgcggccgcc gtggaagccg gcctttggtc cgatatccac
     1981 gaggtagtcg gcggccagca tgatatcctt atcgtgttcg acgacaagga cactgttacc
     2041 gatgtcgcga agatttttca atgcctggat cagctgatgg ttatcgcgct ggtggaggcc
     2101 gatgctgggt tcatcgagga tataggtgat gccctgcagt tgggagccga tctgggtggc
     2161 aagccggata cgctgggact cgccgccgct cagcgtacgg gaagagcggt cgagcgtgag
     2221 ataggtgagg cccacatcgg agaggaactg cagccgctcc ctgatctcct tcaggatatc
     2281 cttggcgatg atgttctgtt tattgttcag tttcttttcc acgccgtcga accattctat
     2341 gaggcgattg aggttcatgg cgctgagttc ggcgatgttc ttctccccta cccggaacca
     2401 gaggctttcc ttgcgcaggc gtgtgccgcc gcaggtgccg cagctcttca gctccatgaa
     2461 acgttcgacc cattcgcgga gcgcatcggt ggtgccgccg gagaaccagc gtttcaaggc
     2521 cgggatgatg ccctcgtagt cggccgtata gatctgcccc acgctggcgt tctcgtcgaa
     2581 ttcgacatcg agggtcgtat cgccggcttc attgccgtaa agcacgagat tcagcgcttt
     2641 cttcggcatg tcctttatcg gggtgtcgag ggagaaccgg cccttcttcg ccaggccctg
     2701 cacctggcgg aacatatacg cttccctttc cccgccgagg gggacgatgc cgccgtcgtt
     2761 gatacttttt gacgggtcgg ggatgatcgc gtccaggctg atctggtaca ccacgcccag
     2821 gcccttgcag gtgggacagg ctccgtaggg ggagttgaag gagaaactgt ttggcgaagg
     2881 ttcctcatag ctgatgcccg tgtcctcgca catcaactgt ttgctgtatt gaaagacctt
     2941 gttatcgtca ttcagcagca ggaagaggag gtctttcccc atttgcaggg ctttctgaac
     3001 actctggctt agccggggct tcatatcttc tgtaaccgcc agcctgtcga taacgacttc
     3061 gatgtcgtgg atcttatagc gatcgacctg catcttggga gcgaggtctt ttacttcgcc
     3121 gtcgacgcgg accttcagat agcctttttt gcggatgtcc tcgaacaatt cccggtagtg
     3181 tcccttacga ccgcggacga gaggggcgag gaccgagatc tttttattcc tgaatttttt
     3241 g
//
LOCUS       DATGFG010000317         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_30134, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000317 DATGFG010000000
VERSION     DATGFG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30134"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..985
                     /locus_tag="VKQ52_05925"
     CDS             134..985
                     /locus_tag="VKQ52_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HLZ86752.1"
                     /translation="MHKFIAAVVLSGVLIGLVAGCRSTKKIQKVIATTAIHKDTTGDA
                     ARTAAAPPRDLHADSMEVIRQTVAGMSRTHIDFQTFSAHMHVHYEGSDGKDNEFNAYV
                     RIKKDSMIWVSINAILGFEAFRLVITPDSVKILDRLKKIVRLRSVSYIQEQVHLPVDF
                     KTLQDLLIGNPIYLDTAHIQFYRTDGKGISLSSVGNVFRNYLTLNTDYTLQHSKLDDM
                     DPLRARTADMTFGEYDHGNGMNFSTYRRISVAEKTKVDIEINVKQFKFNEAFSYSFSI
                     PKNYKRR"
     gene            1173..>2285
                     /locus_tag="VKQ52_05930"
     CDS             1173..>2285
                     /locus_tag="VKQ52_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86753.1"
                     /translation="MLKKLVTAVFLLLAFAGRIFAQQAQPPAQSRADLEKERAAIQKE
                     IEDVRRTLDETHKNKRETLGQLALLQRRLHLRESAIRNINAQIDVIQGDMNESWREIL
                     KLRAELDTLRVQYAQSIVFAYKNRSSYDFLNFIFSAGSFNDALRRIQYLKSYRAYREE
                     RAENIRRTQELLQSKIDGLKVTRQAKDEALKRQSKERLLLEDEKKEKDAMVNRLQSQE
                     KELKKQMVAKQRQDAKLSSAINAAIKRARDLAIKEAAKKNADAKAASDRAAAADRANA
                     AKNSRPDNTSDAAVAPATLNTVKAPAKSVFSSEEDMHLTGDFEINKRRLPWPVSGTIS
                     MSFGRHEYIKGIWHDNAGITIDVASGAAVKSVFKGEV"
BASE COUNT          530 a          656 c          686 g          413 t
ORIGIN      
        1 atcccggcga ccggatcgcg cagatggtgg tcagcccggt gatccagatc ggctgggagc
       61 gtgtggacaa tatcggcgag acggcgcgca gcgcgggcgg gttcggtcat actggtaaat
      121 cttaatttta tccatgcata aattcattgc ggccgtcgtc ctcagcgggg tattgatcgg
      181 tttggtggca ggctgccgtt ccacaaagaa gatacagaag gtgatcgcga cgacggccat
      241 ccacaaggat acgacggggg atgccgcccg gacggccgcc gcgccgccgc gtgatctcca
      301 tgccgactcg atggaagtga tccggcagac cgtcgcgggg atgagccgga cccacatcga
      361 ttttcagacc ttttcggcgc atatgcacgt ccattatgag ggcagcgacg gcaaggacaa
      421 cgagttcaac gcctatgttc ggatcaagaa ggatagtatg atctgggtat cgatcaatgc
      481 gatcctgggt ttcgaggcct tccgcctggt gatcacgccg gacagcgtga agatcctcga
      541 ccggctgaag aagatagtcc gtctgcggtc cgtgagttat atccaggagc aggtacacct
      601 gcccgtggac ttcaagaccc tgcaggacct cctcatcggc aaccccattt acctggacac
      661 ggcgcatatc cagttctacc ggacggatgg caaggggatc tcgctgtcga gcgtcggcaa
      721 tgtctttcgt aactatctta ctctcaacac cgattatacg ctgcaacaca gcaagctgga
      781 cgatatggac ccgctgcggg cccgcacggc cgatatgacc tttggggagt acgaccacgg
      841 caacgggatg aatttctcca cctaccggcg gatctcggtg gcggagaaaa cgaaggtgga
      901 tatcgagatc aacgtgaagc agttcaagtt taacgaagct tttagctatt ctttttcgat
      961 tccgaaaaat tataagcgga ggtaagcgtt agaagggctg gaagcccttc ggccgcaacc
     1021 gagccccgcg aaggttgcga acgagctctt gccggtggca agagcgaagt cctccagatt
     1081 ttagcgggag ggaggatcgc gtagcggtcc gaccgctggt tggatggggg cggcccatcg
     1141 atttgcccaa ttttcattaa cttaaaagga tcatgctcaa gaaactcgtc accgccgtat
     1201 tccttctcct ggcctttgcc ggccggatat tcgcgcagca ggcacagccg cccgcgcaga
     1261 gtcgcgccga cctcgaaaag gaacgtgccg ccatccagaa ggagatcgag gacgtgcggc
     1321 ggaccctcga cgagacccat aagaacaaac gggagaccct tggccagctg gcccttttac
     1381 aacgccggct tcacctgcgg gagtctgcga tccggaatat caatgcacag attgatgtga
     1441 tccaggggga tatgaacgag tcctggcgcg agatcctgaa gctgcgggcc gagctggaca
     1501 ccctgcgggt ccaatacgcc caaagcatcg tctttgccta taagaaccgc agcagctatg
     1561 acttcctgaa cttcattttt tccgccggca gctttaacga cgccctccgg cggatccagt
     1621 atctcaagtc ttaccgtgcc taccgcgaag agcgggccga gaatatccgc cgcacccagg
     1681 aactgctcca gagcaagatc gacgggctga aggtgacgcg ccaggcgaaa gacgaggcgc
     1741 tgaagcggca aagcaaggag cggctgctgc tcgaggacga gaagaaggag aaggacgcga
     1801 tggtcaaccg gctgcagtcc caggagaagg agttgaagaa gcagatggtc gccaagcagc
     1861 ggcaggatgc caagctgagc tccgccatca atgccgccat caaacgggcg cgtgacctgg
     1921 cgataaaaga ggccgcgaag aagaacgccg atgcgaaggc cgcctccgac agggctgccg
     1981 ccgcggatag ggcgaacgcc gcgaagaata gccggcccga taataccagc gatgccgccg
     2041 tggcccccgc cacgctcaat acggtgaaag cccccgccaa atccgtcttc tccagcgagg
     2101 aggacatgca tctcaccggc gacttcgaga tcaacaaacg ccgcctcccc tggccggtgt
     2161 cgggaaccat ctcgatgtcc tttggccggc acgaatatat caagggtatc tggcacgata
     2221 atgccgggat caccatcgat gtcgcctccg gcgccgccgt aaaatccgtc ttcaagggcg
     2281 aggtg
//
LOCUS       DATGFG010000318         6340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1653933, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000318 DATGFG010000000
VERSION     DATGFG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653933"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VKQ52_05935"
     CDS             <1..731
                     /locus_tag="VKQ52_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLZ86754.1"
                     /translation="GAFVAFHGSTDRSPYPQAGYIVCFVPFQQGKPAGKWEVFADGFT
                     RVDTVVNTSDAVFRPMGLAEGPDGSLYISESNKGRIWRIMYKGDKAKFGDADLAKMEP
                     SRSRTYVKTPDEKLDNMGKGGEMAGHILYTSYCVSCHQRDGKGDNSRYPPLVGSDWVA
                     GDHTRLINVLLYGLQGEIKVNGKTFNGIMPAHGAYLDDHAIASILTYIKRNFNKEIAP
                     ILSEEVTKVRGSVVTPTTRVASTH"
     gene            complement(784..1662)
                     /locus_tag="VKQ52_05940"
     CDS             complement(784..1662)
                     /locus_tag="VKQ52_05940"
                     /inference="COORDINATES: protein motif:HMM:NF017006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HLZ86755.1"
                     /translation="MQPLNSAEKNPLSSLDVWEDDLLERYPDPESIAKGKTTEEYRNY
                     ETPGRDSVREFYRLNHTYQTYDFVQQKRSEFLGFDKKEMSVWDAFDFLNQLVDDSDPD
                     TDLDQFQHLLQTSEAIRADGHPDWMVLAGLFHDMGKVLCLFGEAQWAVVGDTFPVGCA
                     WSDKIVYPEFFQYNPDKDDERYQTKYGVYSPNCGLRNVHLSWGHDEYVYHMLKDYLPE
                     PALYMLRYHSFYSQHREHAYDHLMDDHDREMFKWVNLFNPYDLYSKNPTPPNWTELKP
                     YYEGLVAKYLPAKIKF"
     gene            complement(1675..3177)
                     /locus_tag="VKQ52_05945"
     CDS             complement(1675..3177)
                     /locus_tag="VKQ52_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86756.1"
                     /translation="MWIKLKKGDDGRWTTASWLAVVLPVMVLEWIVFKCCYPYADYFT
                     DSYTYIQAAVQQDAISYRPIGYSLFLRLVHLVSASDTLVVTLQYLVVQGASLGLVLSL
                     RRWCGIGERTVAVLMGFLVLNPLIPYVCNYISSDALFIGLSLIWLTVLMGLLRERGWW
                     RLGLQVALLFLIFHLRYVALFYPAVAALMVLLARRGWVFSVVGVVASVGIVMAGTFLI
                     KRVTKRETGADLFSAFSGWQIANNALNLYPHVPVDTTGLPSAECRELAMDVQRYFQEA
                     ARVRKVVDTAEPTIGAAEREEYPSATTEYMWVRSSPLHRYLRDYRRRNKLSYFDAWNR
                     VGVLYTQYGYFVVRKHPMAFVRWYGWPSAKSFFVSGLDVFAVYNGGSDSVDEVAKDWF
                     HYKSRITTVCSSTGQAKLLAPMPWIYLFLNVAFVGTALLFLPSKARRMRDPVFTGSFQ
                     VASAFLLANACFCIFASPSVFRYQVLPMIVLFVFTVCGISKSRIFSVSSS"
     gene            complement(3213..6218)
                     /locus_tag="VKQ52_05950"
     CDS             complement(3213..6218)
                     /locus_tag="VKQ52_05950"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HLZ86757.1"
                     /translation="MLRQVLCCFVFWLPVLAVTAQMPSLKVDGKESTTVYLQKLDVEV
                     SIDGAIATTVWTMTFKNPTKRVLEGELNFPLPQGISVSRYALDINGRMREAVPVEKEK
                     ATQVFENTERRRIDPGLMEKVDGNTFRTRIYPLNAEGVRTVRIGYEQELSWEDEADLR
                     YRLPLAFREPIEAFSIHITVAGTAARPKFDENTREALQFDEWRNSWSAKREWTNFQAD
                     QPIAIRIPQGPGIGGVQMRMVGNHYFFVASVFPKAKAVAKPLPHHVGLLWDVSLSGLR
                     RDHKKEFELLDAYFSRIGNVEVSLVEFSNTTSGVQRFSVHAGDWQALKKVLENAVYDG
                     GTEFGALDLAGYPVDEFLLCSDGHSSFGSDRMGLGSRPVYAIVSSADADFPFLQSVVQ
                     RSGGELIDLHAVSVAKGRDLLCTQPLHFLGVKAADGVGECYPSVSTPVTGALTVAGMV
                     YQPVQKVVLQFGYGKTVVQEEAVALDLIRQQTTDVDLGRLWAQKKIAELDTRYEDNQL
                     EIRHLGRSYGIVTRNTSLVVLENVNDYIVYDIDPPAELREEYDRLLKGRDDNDRWRKR
                     QVSGHAEEYFNELLSWWKGEAPKAPERKEDTEREVRVVQDGNLSMERREQDVAVNAAP
                     PSVGSAPVAGHELRGRAAGVVAPRSSGALQEVVVVGYGTAKRRSVVGSVAKDGEGVGD
                     DAGAGNGGGGEPARGSFTVLKAEANTRYLAELKAAAPGDRYVLYLKLRKEYLYTPLFY
                     FHTAEFFLAAGEKALGVRILSNIAEIDAENYELYKMMGYKLKEIGQVGEACATFRKIL
                     EWRPFEPQSYRDYGLALEDAGLYQNALDTLCLAMMKNYDVAISELYPGIEETLLPEIN
                     ELVHRHKGRLDISRVPRQLLAEMPVDMRVVLNWNRASTDIDLWVTDPDNERCYYGHRS
                     TAMGGRISHDFTRGLGPEQFLLKKAVKGRYKVEVNYYGDTQVRLAGETTLLVEVYTSY
                     GTPQQKRSLVTLQMKPGSKGAVYVGDFEF"
BASE COUNT         1547 a         2264 c         1336 g         1193 t
ORIGIN      
        1 atggcgcctt cgtcgccttc cacggctcca ccgaccgctc cccctacccc caggcaggct
       61 atatcgtctg cttcgttccc ttccaacagg gcaaacccgc cgggaaatgg gaagtcttcg
      121 cagacggctt cacccgcgtc gacaccgtcg tcaacaccag cgacgccgta ttccgcccca
      181 tgggcctcgc cgaaggcccc gacggctcgc tctatatcag cgagtcgaat aaaggcagga
      241 tctggcgcat catgtacaaa ggggataaag caaaattcgg cgacgccgat ctggcaaaaa
      301 tggaacccag caggtcgcgc acctacgtca aaactcccga cgagaaactg gacaatatgg
      361 gtaaaggcgg cgaaatggcc ggccacatcc tctacacctc ctactgcgtc agctgccacc
      421 agcgcgacgg aaaaggtgac aacagccgct acccgcccct cgtcggctcc gactgggtcg
      481 caggcgacca cacccgcctc atcaacgtcc tcctctatgg actacagggc gagataaaag
      541 tgaatgggaa aaccttcaac gggatcatgc ccgcacacgg cgcctacctc gacgaccatg
      601 ccattgcatc gatcctcacc tacataaaaa ggaattttaa caaggagatc gcccccatcc
      661 tttccgaaga agtaacaaag gtccgcggca gcgtagtaac accgacgacc cgggtcgcct
      721 ccacacattg atccccggac gcctcagcac ccatggctcc gcacactgac ccgcggccag
      781 ccgctaaaac ttgatcttcg ccggcaaata cttcgccaca agcccctcat aatagggctt
      841 cagttccgtc cagttcggcg gcgtgggatt cttcgaataa agatcgtagg gattgaacaa
      901 attcacccac ttgaacatct cccggtcatg atcatccatc aggtgatcat acgcatgctc
      961 ccggtgctga gaataaaagg agtgataacg caacatataa agcgcgggct cggggagata
     1021 atccttcagc atgtgataga catattcatc atgcccccag gaaagatgta cattccgtaa
     1081 cccgcagttg ggactgtaaa ccccatactt cgtctggtaa cgctcgtcat ccttatcggg
     1141 attgtattgg aagaactcag ggtaaacgat cttgtccgac caggcgcaac ccaccgggaa
     1201 cgtatccccg acaacggccc actgcgcctc gccgaaaaga caaagcacct tgcccatatc
     1261 atggaaaagc ccggccagca ccatccagtc aggatgccca tccgcccgga tcgcctccga
     1321 ggtctgcagc aggtgctgaa actgatcgag atcggtatcc ggatcactgt cgtctactaa
     1381 ttggttgagg aaatcaaaag catcccacac cgacatttct ttcttgtcga aacccaaaaa
     1441 ttccgagcgc ttctgctgca cgaaatcata ggtctgatac gtatggttca gccggtagaa
     1501 ctcccgcacc gaatcccggc cgggggtctc atagttgcga tactcttccg tcgtcttgcc
     1561 cttggcaata gactcggggt ccggataacg ttccaacaga tcgtcttccc acacatcaag
     1621 agaagataac ggattctttt cggctgaatt aagtggttgc atcgttagca aattttagct
     1681 tgaagatacg gaaaaaatac gggatttcga aatcccacac accgtaaaaa caaacaaaac
     1741 gatcatcggc aacacctggt aacgaaacac actcggcgaa gcaaagatgc aaaaacaagc
     1801 gttggcaaga agaaaggccg acgccacctg gaaactgccc gtaaacaccg gatctcgcat
     1861 ccggcgggcc tttgacggca aaaagagaag cgccgtcccc acaaacgcaa cattcaggaa
     1921 caggtagatc cacggcatcg gcgccagcag cttcgcctgg ccggtggacg aacacactgt
     1981 agtaatccgg ctcttataat ggaaccaatc cttcgccacc tcatcgacac tatcgctgcc
     2041 gccattatat accgcaaaga catccaggcc cgaaacaaaa aagctcttcg cgctcggcca
     2101 gccataccac cgcacaaagg ccatcgggtg cttccgcact acgaaataac catactgcgt
     2161 atacaggacg cccacccggt tccaggcatc gaaatacgaa agcttgttcc gcctacggta
     2221 atcccgcaga taccgatgca gcggactgct ccgcacccac atatattctg tcgtcgccga
     2281 tggatactcc tccctctccg cggccccgat ggtcggctcc gccgtgtcca ccactttcct
     2341 cacgcgcgca gcctcctgga aatatcgttg cacatccatc gccagctccc ggcattccgc
     2401 cgagggcagg ccggtcgtgt ccaccggcac atgcggatac aggttaaggg cgttgttcgc
     2461 gatctgccat ccgctaaaag ccgagaaaag atcggccccc gtctcccgct tcgtgacccg
     2521 cttgatcaaa aacgttccgg ccatcacgat cccgacactc gccaccaccc ccacaaccga
     2581 aaacacccag ccccgccgcg ccagcaacac catcagcgcc gccacggccg ggtagaacaa
     2641 ggccacatac cgtagatgga agatcaggaa cagcaaggcc acctgcaacc ccagccgcca
     2701 ccagccccgc tcccgcaaaa gccccatcaa caccgtcagc cagatcaggc tcagcccgat
     2761 aaaaagcgca tcactcgaaa tataattaca cacatacggg atcagcggat tcaacaccag
     2821 gaaacccatc agcaccgcaa ccgtacgctc cccgatccca caccagcgcc gcaacgacaa
     2881 caccagccca agactcgccc cctgcaccac caggtattgc agcgtcacca caagcgtatc
     2941 cgaggcggag accagatgca ccagccgcag gaacaaagaa tacccgatcg gccggtaact
     3001 gatcgcatcc tgctgcaccg ccgcctggat atacgtataa gagtcggtaa aatagtcggc
     3061 ataggggtaa caacatttga aaacgatcca ctccaacacc atcaccggaa ggacaacggc
     3121 cagccagctc gccgtcgtcc atctcccgtc gtcccccttt tttaatttga tccacatagg
     3181 ttccggccaa aaatagcaca aaactcgctc aactaaaatt caaaatcccc tacatacacc
     3241 gcccccttcg acccgggctt catctgcaac gtaacaagac tgcgtttctg ctgcggggtc
     3301 ccatagctgg tatacacttc caccagtaac gtcgtctccc ccgccagccg cacctgcgta
     3361 tcgccgtaat aattcacttc caccttgtac cgccccttca ccgccttctt cagcaggaac
     3421 tgctccggcc caagcccccg cgtaaaatca tgcgagatcc tgccccccat cgccgtactg
     3481 cgatgcccgt aataacatcg ctcgttatcc ggatcggtaa cccatagatc gatatccgta
     3541 ctcgcccggt tccagttcaa aaccacccgc atatccaccg gcatctccgc cagcagctgc
     3601 cgcggcaccc ggctgatatc cagcctgccc ttatgccggt gaaccagttc attgatctcc
     3661 ggtaacaagg tttcctctat tccgggatac agttcgctga tcgccacatc atagttcttc
     3721 atcatcgcca ggcacaaggt atccagcgca ttctggtaaa gccccgcatc ctccagcgca
     3781 agcccatagt cgcggtagct ctgcggctcg aaaggccgcc attccaggat cttccggaag
     3841 gtggcacagg cttcgcccac ctgccctatc tccttcagct tataccccat catcttgtac
     3901 aactcgtaat tctccgcatc gatctcggcg atattcgaaa ggatcctcac cccaagggcc
     3961 ttttcccccg ccgccagaaa gaactccgcc gtgtggaaat agaacagcgg cgtatacaga
     4021 tactccttgc gtagcttcag atacaacaca tagcggtccc ccggcgccgc cgccttcagc
     4081 tccgcgagat aacgcgtatt ggcctccgcc ttcaacaccg taaaactccc ccgcgccggc
     4141 tcgccgccgc caccatttcc ggcgccggca tcatcgccaa ccccttcccc atctttagca
     4201 accgaaccga ccacgctcct cctcttcgcc gtcccatacc cgacgactac cacctcctgc
     4261 aacgcacccg acgaccgggg cgccacaaca ccagcagcac gccccctcaa ctcatgcccg
     4321 gccacaggtg ccgacccaac cgatggtggc gccgcattca ccgccacgtc ctgctcacgc
     4381 ctctccatcg ataaattccc gtcctgcacc acccgcactt ctctctccgt atcctccttc
     4441 ctctccggcg ccttcggcgc ctcccccttc caccaactca acaactcatt aaaatactcc
     4501 tccgcatgcc ccgacacctg cctcttcctc caccggtcat tatcatccct cccctttaac
     4561 aaccggtcat actcctcccg caactcggcc ggcggatcga tatcatacac gatataatca
     4621 ttgacattct ccaacaccac cagcgacgta ttccgcgtca cgatcccata actccggcca
     4681 agatgccgga tctccaactg gttgtcctca taccgcgtat ccagctccgc gatcttcttc
     4741 tgcgcccaca accgccccag atcgacatcc gtcgtctgct gccgtataag atcaagcgcc
     4801 accgcctcct cctgcacaac cgtcttccca tacccaaact gcaaaaccac cttctgcacc
     4861 ggctgataga ccatccccgc caccgtcaac gcacccgtca ccggcgtgga caccgacgga
     4921 taacactccc cgacaccatc cgccgccttc accccaagga aatgcagcgg ctgcgtacac
     4981 aacagatccc tccccttcgc caccgacacg gcatgaagat cgatcagttc ccctccgctg
     5041 cgctggacca cggactgcag aaagggaaaa tccgcatcgg ccgaagaaac gatcgcatac
     5101 accggccggc tccccaaccc catccggtcg ctcccaaaac tcgaatgccc atcgctgcaa
     5161 agaaggaact catccacggg ataacccgcc aggtccagcg cgccgaactc cgttccccca
     5221 tcatacaccg cattctccaa caccttcttc aatgcctgcc agtccccggc atgaacggaa
     5281 aaccgctgca ccccgcttgt cgtattgcta aactccacca ggctcacctc cacattcccg
     5341 atacgcgaaa aatacgcatc cagtaactcg aactctttct tatggtcccg gcgcaacccg
     5401 ctgagcgaaa catcccacaa caacccgaca tgatgcggca acggcttcgc aaccgccttc
     5461 gccttcggaa aaacactcgc cacgaaaaaa taatgattgc ccaccatccg catctgcacc
     5521 ccaccgatgc ccggcccctg cgggatacgt atcgctatcg gctggtcggc ctgaaaattc
     5581 gtccactccc gctttgccga ccaggaattc ctccactcat cgaattgcaa cgcctcccgc
     5641 gtattctcgt caaacttcgg cctggcagca gtccccgcca ccgttatatg aatactaaaa
     5701 gcctcgatcg gctccctgaa cgccagtggc aaccggtaac ggagatcagc ctcgtcctcc
     5761 caggataact cctgctcata cccgatccgc accgtcctca ccccttccgc attgagcggg
     5821 tagatccgcg tgcggaaggt attgccatcc accttttcca tcagcccggg atcgatccgg
     5881 cgccgctcgg tattctcgaa cacctgcgtg gccttctcct tctccaccgg caccgcctcc
     5941 cgcatcctgc cgttgatatc cagcgcataa cgcgaaacac tgatgccctg cggcaaaggg
     6001 aaattcaact ccccctcgag cacccgcttc gtcggatttt taaaggtcat cgtccagacg
     6061 gtggtggcaa tggccccgtc gatggacacc tccacatcca gcttctgaag ataaacggtc
     6121 gtcgactcct tcccatcgac tttcagggaa ggcatctgcg cggtaacggc aagcaccggc
     6181 agccaaaaca caaagcagca caaaacttgg cgtagcataa ctttttttta ctggatgcag
     6241 ccgccacaaa aattacacac ccttttttcc ggccttccgt ccatcccaat acctcctctt
     6301 cccgttttaa ctctttattg acaatcttca catataactt
//
LOCUS       DATGFG010000319         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_299573, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000319 DATGFG010000000
VERSION     DATGFG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299573"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VKQ52_05955"
     CDS             <1..506
                     /locus_tag="VKQ52_05955"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HLZ86758.1"
                     /translation="PGMMLAYAIGRMGCHLAGDGDWGIVSTSPKPAWLSWAPDWVWAA
                     HYPHNVIRQGVYIPGCQVDYCSVLPQGVFPTSFYESVVSLALFFALWAVRRRLTVPGS
                     LFFVYALFTGLERFLIEFIRITPKHVFLGLSLSQAQWISLGFIGIGLAGTGYILTLSA
                     KKAYFYS"
     gene            511..1395
                     /locus_tag="VKQ52_05960"
     CDS             511..1395
                     /locus_tag="VKQ52_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ86759.1"
                     /translation="MSRSILKESPPLTKADCFSLYARHKTEFNFPVHYHEEYELNFIE
                     NAKGVRRVVGSSMEEIDSLELVLVGPNIPHAWFTYKCESESIFEITIQFHRDLFDEVF
                     LKRNQLNFIRNLFERSLKGLSFSRRTIERVAPRIKAMADKEGFDSVLELMAVLHELSL
                     SPDSRTLSEDISYNTDMIYNDSQRIDKVVDFINENFSRTIRLAEAAELVEMAETAFSR
                     FFKAKTGITFIDYLNDIRIGHASRMLIDTTSPIAEIAQECGFTSLTNFNRIFKKRKGF
                     VPKDFREKHGTPNKKKFV"
     gene            1392..3794
                     /locus_tag="VKQ52_05965"
     CDS             1392..3794
                     /locus_tag="VKQ52_05965"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HLZ86760.1"
                     /translation="MMKHIFTLSVLTVALFHSLSTQSAPRPPAAQATPAAPQSSAPPQ
                     WHANWIAVPGDDGRGYGVYYFRKTLPLSSKPAAFPVYVSADNRYKLFVNGTLVSFGPT
                     RNDLYHWNYETVDLAPWLAPGKNTVCAIVWNEADRRPEAQLSFRTDFILQGASPAEEV
                     LNTDKSWKGIRDNGYQPMNDFVFAASAGELIDRNKSVNNWMAATFDDGTWPSAAQLFG
                     GQPKGQGDGFGYILVPSKLPPREMTVQRIPVCRKAEGITLPAGFPAQKASLTIPANTT
                     VTLLLDQTYNTNAYVTLNFSKGAGAGISLRYAESLYIEFNKYGGRKGNRNEVDGKIFS
                     GREDSIISDGTSAQSYTNLNFRTFRYIQLRLHTKDEPLVIDDLFGTFTGYPFGQPSEF
                     TSDDASLAKILDIGWRTARLNAWETYTDCPYYEQLQYIGDTRVQAMITYYNSSDTRLP
                     RNALDLMDQSRLAEGVTLSRYPSRNTQIISTFSLWYIGMLHDYWMYRGDSAFIADKLP
                     GERGILQFFSNYQDAEGSLKNTPYWTFVDWANANGWNFGSPPKSDDGSSSILDLQLLW
                     AYQWAAEMEAKLGIKYYADLYRQKAAQLKATIQRKYWSPARGLYADTKEKTSWSQHAN
                     SLAVLTGMLNPADRLAVGRRMLTDSTLTQCTIYFKYYLHQALTKAGLGDGYLDWLGIW
                     RQNIAAGLTTWTEDSNIDYTRSDCHAWGSSPNIEFFRTVLGIDADAPGFRKVRIEPHL
                     GALTHAGGAIPHPNGHLAVSYRLENGKWNISITLPPNTTGKLIWHGHAYGLAPGDNHF
                     SL"
BASE COUNT          899 a         1261 c          976 g          843 t
ORIGIN      
        1 ccccggggat gatgctcgcc tatgccatcg gccggatggg ctgtcatttg gccggcgatg
       61 gcgactgggg catcgtctca acctcaccaa aaccggcatg gctgtcctgg gcgccggact
      121 gggtctgggc tgcccactac ccgcataatg tgatccggca aggcgtatat attcccggct
      181 gccaggtcga ctattgctcg gtcctgcctc aaggagtatt ccctacttcc ttttatgagt
      241 cggtggtctc cctggccttg ttctttgcgc tctgggcggt acgccgccgg ttaacggttc
      301 ctggctcgct tttctttgtc tatgccctgt tcacaggtct ggagcggttc ctcatcgagt
      361 tcatccggat cacgccaaaa cacgttttcc tgggactttc actctcccag gcgcaatgga
      421 tctcactggg atttatcggc atcggcctcg ccggcactgg ttatatttta accttatctg
      481 caaaaaaagc ctacttttat tcttaattct atgtcgcgct ctatcctgaa agaaagtccc
      541 ccgctgacaa aagccgattg cttttcgctc tatgcccggc acaagaccga attcaatttc
      601 cccgtgcatt atcacgagga atacgagctc aatttcatcg aaaatgccaa aggcgtaagg
      661 cgggtggtcg gcagcagcat ggaagagatc gatagtctgg aactggtgtt ggttgggccc
      721 aatattcccc atgcgtggtt tacatataaa tgtgaaagcg agagcatttt tgaaattacg
      781 atccagtttc accgcgatct gttcgatgag gtcttcctca aacgaaacca actgaatttt
      841 atccgcaatc tgttcgagcg atcgctgaaa ggcctgtctt tctcccgccg gacgatcgag
      901 cgggtcgcgc cccggatcaa ggcgatggcg gataaggaag gtttcgattc cgtgctggag
      961 ctgatggccg tcctccacga actctcctta tcgcccgact cgcggacgct gagcgaagat
     1021 atcagctaca acaccgacat gatctacaac gacagtcagc gaatagacaa ggtcgtggat
     1081 ttcatcaacg aaaatttcag caggaccatc cggctggccg aagccgccga actggtggag
     1141 atggcggaaa cagccttcag ccggttcttc aaggcgaaga cgggtatcac cttcatcgat
     1201 tatctcaacg acatccgcat cggtcatgct tcccggatgc tgatcgatac gacgagccct
     1261 attgccgaga tcgcccagga atgcgggttc accagtctca cgaatttcaa ccgcatcttc
     1321 aaaaaaagaa agggcttcgt tcccaaggat ttccgcgaga aacatgggac gcccaacaag
     1381 aagaaatttg tatgatgaaa catattttta ccctatcggt cctcaccgtc gcgctgttcc
     1441 actccctctc tacacaatcc gccccacggc cacccgccgc ccaagccacc cccgccgccc
     1501 ctcaatcgtc cgcccctccc caatggcacg ccaactggat cgcggtaccc ggcgacgacg
     1561 gccgcggata tggcgtctac tatttccgga aaaccctgcc cctttcctcc aaacccgccg
     1621 ccttccccgt ctatgtctca gccgacaatc gctacaagct ctttgtcaac ggcacactcg
     1681 tatccttcgg tcccaccagg aacgatctct accattggaa ttatgaaacg gtcgacctgg
     1741 ctccatggct ggccccgggg aaaaataccg tatgcgccat cgtctggaat gaagcagatc
     1801 gccggcccga agcgcagctg agtttccgca cggactttat ccttcagggc gcctctccgg
     1861 ccgaagaggt gctgaacacg gataaaagct ggaaaggcat ccgggataac ggctatcagc
     1921 ccatgaacga tttcgtcttc gccgccagcg ccggagaatt aatcgaccgc aacaaatcgg
     1981 tgaacaactg gatggcagcg actttcgacg acgggacctg gccctctgca gcccaactct
     2041 tcggcgggca gccgaaaggc cagggagatg gatttggcta tatcctggtt ccttcaaagc
     2101 ttccgcccag ggaaatgacg gtccagcgta ttcccgtctg ccggaaagcc gagggcatta
     2161 ccctgccggc cggatttccg gcacaaaagg cctcgctcac gatacccgcg aatacgacag
     2221 tcaccctgct cctcgatcag acctataaca caaatgccta tgtgacgctg aatttcagca
     2281 aaggcgcggg tgccggcatc tccctgcgct atgccgagtc cctgtacatc gagttcaata
     2341 aatatggcgg gcgtaagggc aaccgcaacg aggtcgatgg aaagatcttc tccggtcgcg
     2401 aagacagcat catctcggat ggcaccagcg ctcagtccta caccaatctt aatttccgga
     2461 cattccggta tatccagttg cggctgcaca ccaaagacga gccccttgtc atcgacgatc
     2521 tcttcggcac ctttaccggt tatccctttg gacagccatc ggaatttact tccgacgatg
     2581 cctcgctggc aaagatcctg gacatcggct ggcgtaccgc ccggctgaat gcctgggaaa
     2641 cctataccga ctgcccttat tatgagcagt tgcagtacat cggcgacacc cgggtgcagg
     2701 ccatgatcac gtactacaac agtagtgata ctcgccttcc gcgtaatgcg ctcgacctga
     2761 tggatcaatc gcggctggcc gaaggcgtga ccctcagccg gtatccatcg cgaaacaccc
     2821 agatcatttc caccttttca ctatggtata tcggcatgtt gcatgattac tggatgtacc
     2881 ggggtgacag cgccttcatc gcggataagc tacccggcga aaggggcatc ctccaatttt
     2941 tcagcaacta ccaggatgcc gaaggatcgt taaagaatac accctactgg acatttgtcg
     3001 attgggcgaa cgccaacggc tggaacttcg ggtcgccgcc aaagagcgat gacggctcat
     3061 cctccattct cgatctgcag ctgctctggg cctaccagtg ggccgccgaa atggaggcaa
     3121 agctgggaat aaaatattat gcggatctct atcgccagaa agccgcgcag ctcaaagcga
     3181 cgatccagcg caaatactgg agccccgcgc gcgggctata tgcggataca aaggaaaaaa
     3241 cctcctggtc tcagcacgcc aactccctcg ccgtcctcac gggtatgctc aacccggccg
     3301 ataggctcgc cgtaggccgc cggatgctca ccgacagcac gctgacccaa tgcaccatct
     3361 attttaaata ctatctccac caggccctga caaaggctgg actcggcgac ggctacctcg
     3421 actggctcgg catctggcgc caaaacatcg cggcgggcct caccacctgg accgaagact
     3481 ccaacatcga ctacacccgc tccgactgtc atgcctgggg tagcagcccc aacatcgaat
     3541 tcttccgcac cgtcctcggc atcgacgccg acgcccccgg tttccgcaag gtccgcatcg
     3601 agccccatct cggcgccctc acccacgccg gcggcgcgat tccccacccc aacggtcacc
     3661 tcgccgtctc ctatcgcctc gaaaacggga aatggaacat ctcgatcacc ctcccgccca
     3721 acacaacagg caaactgatc tggcacggcc acgcctacgg gctcgccccc ggcgacaatc
     3781 acttttcgct ttgacctatt ctcgtcaccg cacttttctt aactgatcca ggtgaagacg
     3841 catgccgcca aggtcacgct catccaccgc ctggatcttc cctctccctg gccaatggta
     3901 tcgtcggtct gtaaaaccac cggtcaacga caacatcgac tggccagtat actccaaaaa
     3961 gaccacggga tccatcctg
//
LOCUS       DATGFG010000320         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_650995, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000320 DATGFG010000000
VERSION     DATGFG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650995"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VKQ52_05970"
     CDS             <1..319
                     /locus_tag="VKQ52_05970"
                     /inference="COORDINATES: protein motif:HMM:NF044671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86761.1"
                     /translation="VQATIHWVSVPHAITTEVRLYDRLFRVEDPANEERDFKEYVNPD
                     SLQVLPAAFAEPALRQAKFKEHYQFLRKGYFYLDRDSSEEKLIFNRTTTLRDTWAKEQ
                     KKA"
     gene            complement(393..803)
                     /locus_tag="VKQ52_05975"
     CDS             complement(393..803)
                     /locus_tag="VKQ52_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86762.1"
                     /translation="MNWSKKMIACATLVIALFTVTSTFAQGGRMMGDTAAMRQRMEAR
                     LAKMKTDLNLTQVQADSMRAISTEFGAKRRDVFMDPANQGLSREERMAKMQPLTDAMN
                     ARIQAVLGADLFKKYQDWEQQNRPQRPGGGGGNR"
     gene            1048..1998
                     /locus_tag="VKQ52_05980"
     CDS             1048..1998
                     /locus_tag="VKQ52_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86763.1"
                     /translation="MYPLLVSLYEFGQFLRILLWISVPLVVLSMLVTTWLHYRRKRRD
                     PEEYLLSLDGGDGISIPILRLSSTEMSGNGQESLHADDGAAGEEEDWGPDQKENIYKG
                     ILWMKEKYEQYRDMADRRYEQLREELARTEKKYQDLLEAGGRPAANTGATAAAANLSA
                     AEFAGAASSSAAASLAASTAAGPGAGEPVPDPLSDVDQASYPDLLEEKNRQIAFLQQQ
                     LDQRIKNFHQAELNGRENKGRMLELEELQLRTRHLLEEKQAYIDQLEGELVAERQKIE
                     ELVAKLQASSQQLLTIYEELDRSLKPTGAPGEPLSSPSEP"
BASE COUNT          450 a          578 c          554 g          423 t
ORIGIN      
        1 ggtgcaggcc accattcatt gggtgagcgt gccgcacgcg atcaccacgg aagtgaggtt
       61 gtacgaccgc ctgttccggg tggaagaccc tgccaacgaa gaacgcgatt tcaaggaata
      121 tgtaaaccct gactcgctgc aggtgctgcc ggcggccttt gcggaaccgg ccctgcgcca
      181 ggcgaaattc aaggaacact accagttcct gcgcaaggga tacttctatc tcgacaggga
      241 tagttcggag gagaagctaa tcttcaaccg gacgaccacc ctgcgggaca cctgggcaaa
      301 ggagcagaag aaagcataaa agagaccggc caggagccgg tctttttttt tatcggttac
      361 ggggcgttac cggtccccac cgccgacagt cgttatcggt tgccaccacc gccaccgggg
      421 cgttgcggcc ggttctgttg ttcccagtcc tggtatttct tgaagagatc ggcgccaagg
      481 acagcctgga tccgcgcatt catggcgtcc gtgagcggct gcatctttgc catccgctct
      541 tcacggctca ggccctggtt ggccgggtcc atgaaaacat cacgtctttt cgcgccgaat
      601 tccgtgctga tagcccgcat ggaatcggcc tgcacctggg taaggttcag atcggtcttc
      661 atctttgcca gacgggcttc catccgctgc cgcatagcgg ccgtgtcgcc catcatccgt
      721 ccgccctggg cgaaggttga ggtgacggtg aaaagcgcta tgaccagcgt agcgcaagcg
      781 atcatttttt tgctccaatt catgattgaa atagttttaa ttgtagttag agcaccaaat
      841 ttgtgctaaa atcccctgtc cttcccggct tttagataga cggcaaccgg ctgtttggcc
      901 tatcggtcaa taaatgcttt tttggcgtga atattgctct ttttctcttc ccgcccgccc
      961 caaccgcaga agccgaagcg tttctttctt ttaatacaac tgcctgcata tcaatatcga
     1021 gagcatctct acttaaacct ctctagcatg tatccactac ttgtcagcct gtatgagttc
     1081 gggcaattcc tgcgtatact tttatggatc tccgtacccc tggtggttct ttccatgtta
     1141 gtgaccacct ggctgcatta ccgccgcaaa aggcgggacc cggaagagta cctgctgtcc
     1201 ctcgatggcg gagatgggat atcgataccc atcctacggt tatcttccac cgaaatgagc
     1261 ggtaacgggc aggaatcgct ccatgcagac gatggcgctg ccggggagga ggaggactgg
     1321 gggccggacc aaaaggagaa tatctacaaa ggcatccttt ggatgaagga gaaatatgag
     1381 cagtaccggg atatggccga cagacgctat gagcagttaa gagaggagtt ggcccgcacc
     1441 gaaaagaaat accaggatct gctggaagcc ggagggcggc ctgccgcgaa caccggcgca
     1501 accgctgccg ccgccaacct ttcagccgcc gagttcgccg gtgccgcaag ctcgtccgct
     1561 gccgcaagcc ttgccgcttc gactgccgcc ggccccggcg ccggcgagcc cgtcccggat
     1621 ccactctccg atgtagacca ggcttcctat cccgatctgc tggaggaaaa gaaccgccag
     1681 atcgccttcc tccagcaaca gttggaccag cggataaaaa actttcacca ggccgagctc
     1741 aatggccggg aaaacaaggg ccggatgctg gaactggaag aactacagct ccgcacccgg
     1801 catcttttgg aagaaaaaca ggcctatatc gatcaattgg aaggggagct cgtggccgaa
     1861 cgccagaaga tagaagaact cgtggccaaa ttgcaggcca gcagccagca gttgctcacc
     1921 atctacgagg aattggaccg gtcgctgaaa ccaaccggcg ccccgggtga accgcttagt
     1981 tctcccagtg aaccgtgatc ggctg
//
LOCUS       DATGFG010000321         5994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_258701, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000321 DATGFG010000000
VERSION     DATGFG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258701"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VKQ52_05985"
     CDS             complement(<1..739)
                     /locus_tag="VKQ52_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLZ86764.1"
                     /translation="MSKSFPSGSRSMIETILVVLLLLSLMLAVYDVLRVFFGVLTFAL
                     IFSVSFAGAFEWLVRLVGGRRKLAAVIYTLVLVAIIALPITLIISALRHHVKEAIAWM
                     NDIKTNGLPPLPKWITGLPLVGEDISAFWQQLQNSPRETIATHGEPIKTALRHILTSG
                     AGMVGTALQFIAGIVISAFLLVSGDRILTPIKAVMQHLLGRREGLSLIQATTVAIKSV
                     SIGVMGTAFIASVISWLGMVIAGIHFAV"
     gene            complement(803..2077)
                     /locus_tag="VKQ52_05990"
     CDS             complement(803..2077)
                     /locus_tag="VKQ52_05990"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HLZ86765.1"
                     /translation="MDPTKVSQGLRGSAEVLIPHLFRTEFTRIAAVLCKHFGLEHLEV
                     AEDIAGETFLAALETWPYKGVPPNPTAWLYAIAKNKAKNYLHRDQLFLGKILPQLKKA
                     SGGQAVDEPAEIDLSEKNITDSQLQMLFAVCHPSLSTEAQICLALRILCGFGIDEIAN
                     AFLTNKETINKRLFRAKEKLRTEKTAIEFPPAAELGQRLETVLLTLYLLYNEGYYSES
                     QDAVLREDLCGEAMRLAEQLIQNESTHLPAVDALFSLMCFHASRFPARKKENGEIVLY
                     ADQDESLWDQELIARGAYHLHQASQGDVLSRYHLEASIAYWHTIKTDSREKWENILQL
                     YNRLLTIAYSPIAALNRTFALSKANGKQEAIAAAEKLQLTENPYYYTLLGELYRDIDN
                     GKAMVHLQRAYTLARTQTDKRIIQRAMEGLKA"
     gene            complement(2064..2417)
                     /locus_tag="VKQ52_05995"
     CDS             complement(2064..2417)
                     /locus_tag="VKQ52_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLZ86766.1"
                     /translation="MDEYILIFRHQDGHKVASPEQIQVWMKQTMDWIGGIAAQNKFSG
                     GNGLPFDGAKVVHHNNIVTNGPFGEIKETIGGYIIVKADSVDEAVEFAKGSPVLQGDG
                     NTVEVRKIAKGNGPH"
     gene            2547..3134
                     /locus_tag="VKQ52_06000"
     CDS             2547..3134
                     /locus_tag="VKQ52_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ86767.1"
                     /translation="MQPQLSILPLSNAHRDPVAALVLPIQQTEFNVPITLADQPDLLD
                     LETAYFRPGGHFWGAFVENDLAGTIGLLAVAGTPSPAGVQSLPGAPGGILPAPAGASF
                     GVIRKMFVKPPFRGKPWGVAQQLLETLIAYARQNGMVHLYLGTITRMKAAHRFYEKNG
                     FGRVEKEELPATFPFMPVDDVFYHLSLSSLKEGGS"
     gene            3131..3604
                     /locus_tag="VKQ52_06005"
     CDS             3131..3604
                     /locus_tag="VKQ52_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HLZ86768.1"
                     /translation="MSVIDDSGILAIATRLMRLSEQFRKDGHLVYKEYGIDFEPKWFP
                     VIYALHHKTQLSVVGLAAEIGYTHPSTISLLKELEKQKLIRSQKDKTDERKRMIRLTE
                     KGRDLVTQMEPVWRVFNTAISELIDTPHNLLQAIIEVENNFKEKSLLQRARKHRS"
     gene            complement(3599..4180)
                     /locus_tag="VKQ52_06010"
     CDS             complement(3599..4180)
                     /locus_tag="VKQ52_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HLZ86769.1"
                     /translation="MEKFTPLQETVINRRSTKPAVLNGKKIADGQIRQLLELANWAPT
                     HGNTEPWRFIVYSGEAVQRFCHEHAGLYRQSTPPERFTAAKYEKQQHNGDKASHLIAV
                     YMKRGSNPNITALEEICATAAAVQNILLGAEALGIAVLWSTGGVMLDPIMKEYFELEA
                     EDVLLGLLYLGYTDEPAKPGRRGPVEEKTKWVS"
     gene            4211..4582
                     /locus_tag="VKQ52_06015"
     CDS             4211..4582
                     /locus_tag="VKQ52_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HLZ86770.1"
                     /translation="MGQITLPMGSSSLIVLIFAGVAFAAGGIAVAQFFVKGTKNAQKG
                     QAYECGIPTTGTPWNQFNVGYYLYALIFLIFDVELVFMYPWAVAVKKIGMAGLVEILI
                     FLFILFMGFLYAQKKGALKWM"
     gene            4573..5085
                     /locus_tag="VKQ52_06020"
     CDS             4573..5085
                     /locus_tag="VKQ52_06020"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HLZ86771.1"
                     /translation="MDVNPQKKEPPFPGEVHPTAGGGILVTKLDEVINWARSNSLWPL
                     VFGTSCCAIEMMSTAAAKYDWSRFGFEVARATPRQADVIIIAGTIVNKMAPVLKRLYD
                     QMPDPKYVIAMGACATSGGPFFYNTYSVVKGADHVIPVDVYVAGCPPRPEALLHALIT
                     LQEKIRHGKT"
     gene            5082..5537
                     /locus_tag="VKQ52_06025"
     CDS             5082..5537
                     /locus_tag="VKQ52_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HLZ86772.1"
                     /translation="MNREELKQSLLEILPSATFEEGTEWTTIAIDPAAWEDLALELRS
                     AWGLEFDFLFCVTAVDWKTHFTMVYHLTSTIHRHTLVVKAKLDRQHPEIRTVSDIWRT
                     AEFHEREAYDLMGIVFTGHPDLRRLFMTDEWQGWPLRKDYEDPVNMIKL"
     gene            5571..>5994
                     /locus_tag="VKQ52_06030"
     CDS             5571..>5994
                     /locus_tag="VKQ52_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-quinone oxidoreductase"
                     /protein_id="HLZ86773.1"
                     /translation="MIEEIGTTEAGELVINLGPQHPSTHGVLHLVITLSGETIKKVEP
                     HLGYIHRSIEKMCESLSYRQFIYVTSRMDYLSSHINNHGCALCVEKGMQIEVPPRAKV
                     IRVLMDELTRLASHQLWWGAMAMDVGALTPFFHAFRERE"
BASE COUNT         1320 a         1735 c         1611 g         1328 t
ORIGIN      
        1 ggacggcaaa atggataccg gcgatcacca tcccgagcca tgaaatgacg gaggcgataa
       61 acgccgtccc catcacgccg atggatacgc tctttatcgc cacggtagta gcctggatca
      121 gggaaagccc ctccctcctg cccagcaggt gttgcatcac cgccttgatc ggggtcagta
      181 tcctgtctcc cgagaccagc agaaaggcgg agatcacgat tcccgcgatg aattgcaggg
      241 ccgttccgac catccccgcg ccgctggtaa ggatatgccg cagggccgtt ttgatcggct
      301 cgccatgcgt ggcgatcgtc tcccgcggac tgttctgtaa ttgttgccag aaagcgctga
      361 tgtcttctcc gacaagcggc agccccgtga tccacttcgg cagcggcggc aggccgttcg
      421 tcttgatatc gttcatccag gcgatcgctt ccttcacatg atgccgcagg gcggagatga
      481 tcagcgtaat gggcagcgca atgatggcaa ccagcacaag cgtatagata acggcggcaa
      541 gttttcgcct gccacccacc aaccgcacca gccattcaaa agccccggca aaggaaaccg
      601 aaaagataag ggcaaaggtc agcaccccaa agaagacccg cagcacatca tacacagcca
      661 gcatcaggct gagcaatagc aggaccacca ggatggtctc gatcatactt cgtgaacccg
      721 aagggaaaga tttactcata ttgggttgct tacgcgaaaa accggcctaa atttatactt
      781 atttaggcat tttctgcaat ggtcatgcct tcaacccctc catcgcccgt tggatgatcc
      841 gcttgtcggt ttgtgtcctg gcgagcgtat aggccctttg cagatggacc atcgccttgc
      901 cgttgtcgat atcccggtaa agctcaccca gcaacgtgta gtaatacgga ttttcggtga
      961 gctgtagctt ctccgccgct gcgatcgctt cctgtttccc gttggccttt gacagggcaa
     1021 aggtccggtt cagtgccgcg atcggcgagt aagcgatggt caggagccgg ttatacaatt
     1081 gcaggatatt ctcccatttt tccctgctgt cggtcttgat cgtatgccag taagcgatac
     1141 tggcctccaa atgatatctt gacaggacat cgccctgcga cgcctggtga agatgatacg
     1201 cgcccctggc gatcagttcc tggtcccaga gggattcgtc ctggtccgca tacaggacga
     1261 tctctccgtt ctccttcttt cgggcgggaa aacgcgaggc atggaaacac atcagcgaga
     1321 acagggcgtc gacagccggg aggtgggtgg actcgttttg gatcagctgc tccgcgagcc
     1381 gcatggcttc tccgcaaagg tcttcccgca ggacggcatc ctggctttcg gaataatacc
     1441 cttcgttata caggagatac agcgtgagca gcacagtctc cagccgctga cccagctccg
     1501 ccgcaggagg aaattcgatc gccgtctttt ccgtccgcag tttctccttg gccctgaaaa
     1561 gacgcttgtt gatggtctcc ttattggtga gaaaggcatt cgcgatctcg tcgatgccaa
     1621 aaccgcagag gatgcgcaac gccaggcaga tctgggcttc agtggacagg gagggatgac
     1681 acaccgcaaa cagcatttgc aactggctgt cggtgatatt cttttcagac aggtcgatct
     1741 cggccggctc atcgacagcc tgcccgccgg atgccttttt caactgcggc aggatcttcc
     1801 ccaaaaagag ctggtcgcga tgcaggtagt tctttgcctt gttctttgca atggcataca
     1861 gccaggccgt cggattgggc gggacaccct tgtagggcca ggtctccagt gcggcaagaa
     1921 aggtctcgcc ggcgatatcc tccgccacct cgaggtgttc gagcccgaag tgcttgcaaa
     1981 gcaccgccgc gatcctggtg aattcagtcc ggaacaagtg agggatcaag acctccgcgc
     2041 tgccgcggag gccttgggaa accttagtgg ggtccattgc ctttggcgat ttttcgtact
     2101 tcaacggtat tgccatcccc ctgcaacacc gggctgccct tggcaaactc caccgcctcg
     2161 tcgacggaat cggcctttac gatgatatat ccgccgatcg tctcctttat ctccccaaag
     2221 gggccattcg tgacgatgtt gttgtgatgc accactttgg cgccgtcaaa aggcagcccg
     2281 ttgccgccgg agaatttgtt ttgggcggcg atccccccta tccagtccat ggtttgcttc
     2341 atccaaacct ggatctgttc cggggaagct actttgtggc cgtcctgatg cctgaaaatc
     2401 aagatgtact cgtccatttt tcaatgtttt aaaatgattt gatattgatt acaggactat
     2461 ataaaatgaa gttatgcaaa ttggacaggg cccttgtata attttttatg tagtcaacta
     2521 cgtagattta tacgtatctt tgttgcatgc aaccgcagct gtctatcctc ccgctctcga
     2581 acgcccatcg cgacccggtg gccgcgttgg tattgcccat ccagcagacg gagttcaacg
     2641 ttccgatcac gctggccgac cagcccgatc tccttgacct ggaaaccgcc tacttccgcc
     2701 ccggcggcca tttctggggc gccttcgtag agaacgattt ggccggcacg atcggcctgc
     2761 tggccgtggc cggcacgcct tcccccgccg gggtccagtc cttgcccggc gctcctggcg
     2821 ggatcctccc ggccccggcc ggcgcctcct ttggcgtcat ccgcaaaatg tttgtaaaac
     2881 ccccgttcag gggcaaacca tggggcgtcg cgcagcaact gctggaaacc cttatcgcct
     2941 acgcccggca aaacgggatg gtgcatctat atttaggaac gataacccgg atgaaggccg
     3001 cccaccggtt ctacgaaaag aacgggttcg gcagggtaga gaaggaagaa ttgcccgcca
     3061 cattcccgtt catgccggtg gacgacgttt tctatcattt gtccttatca tcattaaaag
     3121 aagggggatc atgagcgtca tcgacgattc gggcatattg gccatcgcaa cccgcctgat
     3181 gcggctcagc gagcagtttc gcaaggacgg ccacctcgtc tataaggaat acggcatcga
     3241 tttcgaacca aaatggttcc ccgttatcta tgccctccac cacaagaccc agctcagcgt
     3301 cgtcggactc gccgcggaga tcggttatac ccatccctcc acgatcagcc tgctcaagga
     3361 actggaaaaa caaaaactga tccggagtca gaaagacaag accgacgaac gcaaaagaat
     3421 gatccgcctc acggaaaaag gccgggacct cgtgacgcag atggaacccg tctggcgcgt
     3481 gttcaacacc gccatctcgg agctcatcga tacaccgcac aatctcctgc aggcgatcat
     3541 cgaggtcgag aacaatttca aggagaaaag cctgttgcag cgggcaagaa agcaccggtc
     3601 atgagaccca ttttgtcttt tcttccaccg gtccgcgcct gccgggcttc gccggctcat
     3661 ccgtataccc cagatacagg agccccagca gtacgtcttc cgcttccagc tcaaaatact
     3721 ccttcataat gggatccagc atgaccccgc cggtactcca caggacggcg atgccaagcg
     3781 cctccgcccc aagcaggata ttctgtacgg ctgcagccgt cgcgcagatc tcttccagcg
     3841 cggtgatatt ggggttagaa ccccgtttca tatagacggc gatcagatgc gaagccttgt
     3901 cgccattgtg ttgctgtttc tcgtatttgg cggcggtaaa tcgctcgggc ggcgtgctct
     3961 gccggtacag tccggcatgc tcgtgacaaa accgctgaac cgcttctccg ctgtacacga
     4021 taaaccgcca gggttctgta ttcccgtgcg ttggggccca gttggcgagc tccaataatt
     4081 gccggatctg cccgtcggct atcttttttc cgttcaggac tgcgggcttt gtgctgcgtc
     4141 tgttgatcac tgtctcttgc agtggcgtaa atttctccat ggtggtgcaa aaataaagcc
     4201 ttatattagt atgggtcaaa tcactttacc catgggaagc tcttcgttaa tcgttcttat
     4261 tttcgccggg gtcgcctttg cagccggggg tatcgccgtt gcgcagttct ttgtaaaagg
     4321 tacgaagaat gcgcagaaag gacaggccta cgagtgcggc atccccacca ccggcactcc
     4381 ctggaatcag ttcaatgtag ggtattattt atacgcgctc atcttcctca tttttgacgt
     4441 agaactggta ttcatgtatc cctgggccgt ggcggtcaaa aaaataggga tggcaggact
     4501 tgtggagatc cttatcttcc tgttcatcct gttcatgggt tttttgtatg cacagaaaaa
     4561 gggagcgttg aaatggatgt gaacccacag aaaaaagagc cgcccttccc cggggaagta
     4621 catcccaccg cgggtggcgg catcctggtg accaaactgg acgaggtgat caactgggcc
     4681 cggagcaatt ccctctggcc cctggtcttc ggcaccagct gctgcgccat cgagatgatg
     4741 tccaccgccg ccgcgaaata tgactggtcg cgttttgggt tcgaggtggc aagggccacc
     4801 cccagacagg cggatgtgat catcatcgcc gggactatcg tgaacaagat ggcccccgtg
     4861 ctcaaacggc tctacgacca gatgcccgac cccaaatatg tgattgccat gggcgcctgc
     4921 gcaaccagcg gcggcccgtt cttctataat acctattcgg tggtaaaagg cgcggatcac
     4981 gtgatcccgg tggatgtcta tgtggcggga tgtccacccc gcccggaggc cttgctgcat
     5041 gccctgatca ccctgcagga aaaaataaga cacggaaaaa catgaacagg gaagaactaa
     5101 aacaaagcct gctggagatc cttccttcgg ccaccttcga agaagggacg gaatggacga
     5161 ccattgccat cgaccctgcc gcatgggagg acctggcgct ggaactccgc tccgcctggg
     5221 ggctcgaatt cgactttctg ttttgtgtga cggcggtcga ctggaagacc catttcacca
     5281 tggtctatca cctgacatcg accatccatc gccataccct ggtggtgaaa gcaaaattgg
     5341 atcgtcaaca tccggagatc cgcacggtgt ccgacatctg gcgcacggcg gaattccatg
     5401 agcgcgaggc atatgatctc atgggcatcg tcttcaccgg tcaccccgat ctgcgaagac
     5461 tgttcatgac cgacgaatgg cagggatggc cgctgcgcaa ggattatgag gatccggtca
     5521 atatgatcaa actataacgt gctcgaacta taacgtgatc gaacgatact atgatcgaag
     5581 aaataggcac aacggaagca ggggaactgg tgatcaacct gggcccgcaa catccatcca
     5641 cgcatggcgt gctgcatctg gtgatcaccc tgagcgggga gaccatcaaa aaggtggaac
     5701 cccatctggg gtatatccat cgcagcatcg agaaaatgtg tgagagcctg tcctaccggc
     5761 aattcatcta tgtcaccagc cgcatggact atctttcttc gcatatcaac aaccacggtt
     5821 gcgccctctg cgtggagaaa ggcatgcaga tcgaggtccc gccccgcgca aaagtgatcc
     5881 gtgtgctcat ggatgagctg acccggctgg cctcgcacca gctctggtgg ggcgccatgg
     5941 ccatggacgt gggcgcgctg acccccttct tccatgcctt ccgggaaaga gaga
//
LOCUS       DATGFG010000322         8980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1515915, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000322 DATGFG010000000
VERSION     DATGFG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515915"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VKQ52_06035"
     CDS             complement(<1..889)
                     /locus_tag="VKQ52_06035"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HLZ86774.1"
                     /translation="MNPRSSLRPALVLMLVFVHTAVFAQQQDLLGLVEDKSPKKEFVT
                     NAFKSTRVIDGQSIEFLGKGVLDTRILHRFGLVNTGATNLYGLDQANMRLGFDYGLLK
                     SLMLGIGRTNVNKELDGFIKFRPVWQSSGPGGSPFSLVIVTAMTMATGAWADTTRKNF
                     YTSRLSFYNAVIIGRKFSNRFSLQLTPEMVHRNLVTFASDHNDVFALGVGARLKLSNR
                     VAFVVDYHHIVQGLRPGSYQDPLAIGFDIETGGHVFQLHFSNSLGMNERAFLTETTDN
                     WGKGAIRFGFNLSRVFTVRK"
     gene            complement(895..1455)
                     /locus_tag="VKQ52_06040"
     CDS             complement(895..1455)
                     /locus_tag="VKQ52_06040"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ86775.1"
                     /translation="MKNIISFLLSTCLLLPATSHAQDKFYTKQGRIYFDATGGMEKVE
                     ATSAGAVCVLDTHTGAVQFAVLLKGFEFEKALMQEHFNENYVESDKYPRAEFRGQIIN
                     NQEIAYKKNGTYPARVKGKLTLHGETKEISCEGNLTIKDGRITVTGDFVIPLADYHIA
                     IPSLVKDKVAQQVKIRVDCLLDALNN"
     gene            complement(1464..1928)
                     /locus_tag="VKQ52_06045"
     CDS             complement(1464..1928)
                     /locus_tag="VKQ52_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86776.1"
                     /translation="MKKKKLILLSMFAVVATVALYGFREYYRRNRDLAGIPPDLVIRD
                     TTLLRWFTAGGAGNSYIGHIVQVDGMIRKIEKDEQAHIIVILGASTTPSAVRCSIDSV
                     HIGEIGGWQTGHRVKIKGMLVGFNPDETGLLGSDVELNRCVPEQYTSTTSNQ"
     gene            complement(1970..2335)
                     /locus_tag="VKQ52_06050"
     CDS             complement(1970..2335)
                     /locus_tag="VKQ52_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86777.1"
                     /translation="MNKKTIGTAAMLAGFILAFSACYYDKEQLLYPSTTTCGATPSGF
                     AANVLPFIQASCDQGSGCHGAGSGNGPGALTNYTQIKNAGVSIQSSVTAGRMPLGSHL
                     STAQIQMINCWVANGMLNN"
     gene            complement(2368..3108)
                     /locus_tag="VKQ52_06055"
     CDS             complement(2368..3108)
                     /locus_tag="VKQ52_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86778.1"
                     /translation="MKKLLYSLPIVLFGLLFSVLACRHEIPISIRTGNGSDTLAGPPG
                     SGGGPGNTVVSCSADTVYFVQSVLPLLTSGCAQSGCHNGSSGGDAGDYTLNTYAGIMA
                     LVTPGNAAGSRLISITSSGRMPPRGYAALTADQIAMLTKWINQGAPDNSCTPSTCDTS
                     QVAYASTIVPILQTNCTGCHSGSAAGGGGIDLTQYANVLTQVSSGKLWGDIKHLAGYN
                     AMPLGGGSLSSCDLSKINTWIRAGAPNN"
     gene            3244..3855
                     /locus_tag="VKQ52_06060"
     CDS             3244..3855
                     /locus_tag="VKQ52_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HLZ86779.1"
                     /translation="MPAINNNIAAMVKDEALVWRLIQQKDESAFEAYYKENYQACCLT
                     AYRYLRDRATARQVVNDVFLVLWTQAASIRIETSLRAYIYRAIVNRSLNLLKKERRQT
                     HRLVELIDLPDPTAGTRRMEQTELNARLMKAIDELPEQCGKVFRMSRFQNMKRQEIAD
                     KLGISVKTVQHHIARALADLHRIWDTYSVVWLLLLIELFLKER"
     gene            3894..4937
                     /locus_tag="VKQ52_06065"
     CDS             3894..4937
                     /locus_tag="VKQ52_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ86780.1"
                     /translation="MDPFESYPPPPWDLIAASLNGELTVEEETALNRWLSSSRANQEI
                     FARCRALWEEGLADQGLFTQEDARTDWEQLRESLHRAGRAAPGYGGEVEPVSGGRRQV
                     KKKMIRLAAAAVFAGAILTGGLLWRSHHRETIFTAAPGAPAKLTLPDGTVVELKAGGR
                     LRLDREFHSGNRNVFLETGTATFDIARDPSRVFTIRADSASIRDLGTRVTVQLTDDSV
                     MVTLLNGRVAFSGAPGAGEETKQLRPGTAMAYYPAGHRFGVLILAGLADVTGHNLLRF
                     DRTPLSEVTRVLQEISGRTILLGDTSLRRRQLAARLDGETFDDAIRIICTSLNLECNR
                     EGDTYILKSKADK"
     gene            4934..7519
                     /locus_tag="VKQ52_06070"
     CDS             4934..7519
                     /locus_tag="VKQ52_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86781.1"
                     /translation="MNWKILLLPFVLAAGIAAAQDGPRVDSRVSLEVKDMPWQDVLSL
                     IGSKTNLRFDYDPQLLPTGKLSFHVVDMPVSRLLDQLFRGTGLSYTLIGRGVVLRGRK
                     EPVNITVSGFVRDGQTGEWLIGASIFLPGHDAGAASNDYGFYSLTVPAADSLEVGISY
                     VGYQTLYRRVSGRHDLALTQDLERNEAETRIEPMTLSKDRREENVTRNQTSVVELNSG
                     VAGSALSVGGSGDLVNSLQLVPGVQIGIDGSAGYSVRGGNAGQNLVLLDEATIYNPSH
                     LFGLAGIFPAEAVKYAEFLRGGFPAQYGDHLSSVLNIAMKDGNSQQQGASAQMGTIVS
                     GLQVYGPLSRDHSSYFVSARRSLIDLWAGSFATNDYFSNYYFFDINAKLNFRVSGRDR
                     LLFSFYRGRDQNGYSGEAPAGGGIIYDTHFGNLAGSIRWNHVLSPRLFVNSSLIYNHY
                     EQFLSATQGGYFAQLYSGIRDTRFRSDLSFYVHPGHRISAGIDLLSQVLSPASLSDKI
                     GAGGAGIDVAGIPSKNTVRSALYAGDEIHAGDHWVAYAGIRGARYDADGVSYFNAEPR
                     LSLLYLLNSRTSLKASYSRTDQYLHLVQSYNASFPAEIWVGSSPLLHPQTADEYTAGI
                     FRNIHDNSIQASLEVYYKNMLNQSLFKGVTSPVIDNSLENKLIFGRGWSYGAEWMIRK
                     NRGRWKGWLSYSLAYAWQQFDSLNGGKAFPFALDRRHSVAIATTYELNMHWKASATFY
                     AASGRAFTLATPAAGGQQNPNPLYEDDHGDDGEQAPSSQQDINNYRLSPYNRLDLGLS
                     YNRGHRDHAILKDVTVTLTVYNVYARYNAEFAYRTIDPLTGKAAATQITFIPVIPSLT
                     VGITL"
     gene            7593..8411
                     /locus_tag="VKQ52_06075"
     CDS             7593..8411
                     /locus_tag="VKQ52_06075"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLZ86782.1"
                     /translation="MKHLPATEQIAFYQANGFVVIEDFLAPDELGHWRKTVMQAVAER
                     GGRKIPGKDILIGEDDGINEDGDYFGKVFDQLLNLWQTNTAVKELMLDERIGRMAAML
                     SGAEGIRIWHDQALFKRPWANPTAWHLDNPFWSFYDRRAISIWVALDDATWQNGCLYF
                     IPGSHKGTNFDKITIGRNMDGIFDIYPQYAKTPSVAAPMKAGSCSFHNGLTIHGAGAN
                     MTNAWRRAMTCAYMPDGNVFNGEPNILPEPYLSSLTTGDLLDNNEQNPLIYAAH"
     gene            8398..>8980
                     /locus_tag="VKQ52_06080"
     CDS             8398..>8980
                     /locus_tag="VKQ52_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86783.1"
                     /translation="MQPIERPAVPPRKTAAGYSGRFIHTISILAFLLLSPPANAQLTP
                     RPNAQLTPRHLLEKQIDLAGMQKVLFSKAAWKPFPRSAAEWEKILPDSIRAVLIRNGE
                     ACLQGSFPNVPASVTLDFFRNGNRTRYESIAFGKRNRLWALVLAESVEGRGRFLDAIL
                     DGIWSISEETFWGASAHLFLQKGGKGLPDAENPV"
BASE COUNT         2012 a         2505 c         2555 g         1908 t
ORIGIN      
        1 gcttccggac ggtaaatacg cggctgagat tgaatccgaa acggatggcg cccttgcccc
       61 aattatcggt tgtttcggtg agaaaagccc gttcattcat acccaggctg ttcgaaaaat
      121 gcaattgaaa gacatgcccg ccggtctcga tatcaaagcc aatggccagc ggatcctggt
      181 agctgcccgg gcgcagcccc tgcacaatgt gatgatagtc gactacaaaa gcgaccctgt
      241 tcgatagttt caacctggcg cccacaccaa gcgcaaaaac gtcattatgg tcggaggcaa
      301 aggtgacgag attccggtgt accatttccg gcgtcaactg aaggctgaat cggttgctga
      361 atttacggcc gatgatgacg gcattataaa aggaaagccg ggaagtgtag aagttcttcc
      421 gcgtggtgtc tgcccacgca ccggtagcca tagtcatggc cgtgacgatg acaagcgaaa
      481 aaggagatcc gccgggcccg ctggattgcc atacgggccg gaattttata aacccatcca
      541 attctttgtt gacattggtg cggccgatcc cgagcatcag gcttttcagc aacccataat
      601 cgaatcccaa acgcatattg gcctggtcga gaccataaag gttagtggcg ccggtgttca
      661 ccaacccgaa gcggtggagg atgcgggtgt cgagcacgcc cttgccaagg aactcgatgg
      721 actggccatc gatgacgcgc gtggatttaa aggcattggt cacaaactcc ttctttggcg
      781 atttgtcctc gacaaggccc agcaggtcct gctgctgcgc gaatacggcc gtatggacaa
      841 acaccagcat aagaacgagc gccgggcgca aagatgatct cggattcatg gatactaatt
      901 gtttagtgca tcgagcaggc aatccacccg gatctttact tgttgcgcga ccttgtcttt
      961 gaccaggctc ggaatcgcga tgtgataatc ggccagggga atgacaaaat cgccggttac
     1021 ggtgatcctt ccgtccttga tggtaaggtt tccttcacag ctgatctcct tcgtttcgcc
     1081 atgcagggtc agcttgccct ttacgcgggc agggtaggtt ccgttctttt tgtaggcgat
     1141 ctcctggttg ttgataattt gtcccctgaa ttccgcacgg ggatatttat cgctctccac
     1201 ataattttcg ttgaagtgtt cctgcatcag ggctttttcg aattcgaatc ctttgagcag
     1261 caccgcaaac tggacggcgc cggtatgcgt gtcgagcacg caaaccgcac ccgcgctggt
     1321 cgcctccacc ttttccatgc cacccgtggc gtcgaaatag atccgcccct gtttggtata
     1381 gaatttatcc tgggcatggc tggtcgcggg gagaaggagg caggtcgaca agaggaagga
     1441 aatgatgttt ttcatattgt ttcttattga ttcgaagtgg tggaagtata ctgttcggga
     1501 acgcagcggt tgagttcgac atccgaaccc agcaacccgg tctcatcggg attgaagccg
     1561 acgagcatgc ctttgatctt cacccgatgc cctgtttgcc agccgccaat ctcgcctata
     1621 tgaacactgt cgatggaaca gcggacagcc gaaggggtcg tcgacgcgcc aaggatcaca
     1681 ataatatgtg cctgctcatc cttttcgatc ttcctgatca tcccgtccac ctgcacgata
     1741 tgcccgatat aactgttgcc ggcgccgccg gcggtgaacc atcggaggag ggtcgtgtcc
     1801 cggatgacaa gatccggggg aataccggca aggtccctgt tacggcgata gtactccctg
     1861 aaaccatata acgcgacggt tgccacaacg gcaaacatcg acaggagaat gagttttttc
     1921 tttttcatgg tcatcggttt gaaacctggc tgattatttt gacactggcc taattattca
     1981 acatgccgtt ggccacccag cagttgatca tctgtatctg tgccgtggat aaatgcgaac
     2041 ccaggggcat cctgcccgcc gtcacggacg actggatact cacaccggcg ttctttatct
     2101 gggtgtagtt ggtaagcgct ccgggaccgt tcccgctgcc cgcaccatgg caccccgacc
     2161 cctgatcgca acttgcctgg ataaagggca gtacgttggc agcaaagccg gacggtgtgg
     2221 cgccgcaggt tgtcgtggaa ggatacagaa gttgttcctt gtcataataa caggcgctga
     2281 atgccaggat aaagcccgcg agcatcgccg ccgtaccgat tgtcttcttg ttcatgagct
     2341 gatttttgct ttcatagccg gttactgcta gttgttgggc gcgccggccc tgatccaggt
     2401 attgatcttg ctcagatcgc aggagctaag gctgccgcca ccgaggggca tggcattata
     2461 gccggcgaga tgcttgatgt ccccccataa tttaccggaa ctcacctgcg tcagtacatt
     2521 ggcatattgc gtgaggtcga tcccgccgcc gccggccgcg ctgccgctgt gacagccggt
     2581 acaattcgtt tgcaggatag ggacaatagt cgacgcatag gccacctggg atgtatcgca
     2641 ggtgctcgga gtgcaactat tgtccggggc gccctggttg atccatttag tgagcatggc
     2701 gatctggtcg gccgtcaggg cagcatatcc cctgggcggc atgcgaccgg aagaggtgat
     2761 cgatatcagg cggctgcctg cggcattgcc gggtgttacc agggccatga taccggcata
     2821 cgtattcagg gtataatccc cggcatcccc gccggaagaa ccgttgtgac atccgctctg
     2881 cgcacagccc gatgtcagca ggggcagtac cgactgaaca aaatagacgg tatcggcact
     2941 acaggaaaca accgtattgc cggggccgcc cccggacccc ggcggcccgg caagcgtgtc
     3001 acttccgtta cccgtcctga tggaaatggg tatttcatgg cggcaagcga gaacgctgaa
     3061 caaaagcccg aacaatacga tgggcaggga ataaagaagc ttcttcatgt aattcaggtt
     3121 tttagacagt cattggatca tttgttccgc cgaagaggcg gggttactgt ttacgacaac
     3181 ctctaagcgc cggtggccta gtcggcggca aaatattttt tcacctgacg ataatcattg
     3241 gagatgccgg caataaataa taatattgcc gcaatggtca aggacgaagc actcgtctgg
     3301 aggctgatcc agcaaaagga cgaatcggca tttgaagcct attacaagga aaattatcag
     3361 gcctgttgcc tgacggccta ccgatatctc cgcgatcggg cgactgcccg gcaggtggtc
     3421 aacgatgttt ttctcgtgct ctggacacag gcggcatcca tccggatcga gacctcgctt
     3481 cgtgcctata tttacagggc gatcgtgaat cgaagcctga acctgctgaa aaaagaacgg
     3541 aggcaaacgc ataggctggt ggagctgatc gacctgcccg atccgacagc cgggacaaga
     3601 cggatggagc aaaccgaact gaatgcccgg ctcatgaagg cgatagacga attgcccgag
     3661 caatgcggca aggtattccg gatgagccgc ttccagaata tgaagcgcca ggagatagca
     3721 gacaagctcg gcatctctgt caagacggtg cagcatcata ttgcccgggc gctggcagac
     3781 ctgcaccgta tctgggatac ctactccgta gtctggcttt tgttgctgat agaattattt
     3841 ttaaaggaac gataggccgt ctgcctgttt attttgtcta attgatacct gatatggacc
     3901 catttgaatc atatccacca cccccctggg atctcatcgc cgcctcactg aacggcgaac
     3961 tcaccgtaga agaagagact gccctgaacc ggtggctttc atcgtcccgg gcgaaccagg
     4021 agatatttgc ccggtgccgc gcactgtggg aagaagggct ggccgatcag ggactcttca
     4081 cacaggaaga tgcccggaca gactgggaac aactgcggga gagcctgcac cgggccggaa
     4141 gagcagcgcc cggatacggc ggcgaagtgg agcccgtgtc cggcgggaga aggcaggtca
     4201 aaaaaaaaat gatccggctg gccgccgccg ccgtctttgc gggcgccatc ctgacgggtg
     4261 gacttctttg gcggtcgcac catcgggaaa cgatatttac cgccgccccg ggcgctccgg
     4321 cgaaactcac cctgccggac ggcacggtgg ttgaattgaa ggccggtggc aggttacggc
     4381 tggacaggga attccattca ggcaacagga atgtcttcct ggaaacagga acggccacct
     4441 tcgatattgc aagggatccc tcgcgggtct ttaccatccg ggccgatagc gcgagcatcc
     4501 gtgatcttgg cacccgggtg acggtacagc tgacggatga cagcgtcatg gtcaccttgc
     4561 tgaacggcag ggtcgctttt tcaggcgccc cgggcgcggg tgaggagacg aagcagctga
     4621 ggcccggcac ggcgatggct tactacccgg ccggccaccg gtttggcgtt ctgatcctgg
     4681 cgggccttgc agacgttaca gggcacaacc tgctgcggtt cgatcgcact cctttgagtg
     4741 aagtaacccg tgtcctgcaa gagataagcg gcaggacgat cctgctcggg gatacctcgt
     4801 tgaggcgccg gcaactcgcc gcccgtctgg acggggaaac cttcgatgac gccatccgca
     4861 tcatctgcac atcgctaaac ctcgaatgca accgggaggg cgatacctat atcctaaaat
     4921 cgaaagcgga caaatgaact ggaaaatcct tctccttccc ttcgtcctgg cggctggcat
     4981 tgccgctgcc caggacgggc cacgggttga cagtagggtg agcctggaag taaaggacat
     5041 gccctggcag gacgtgttga gcctcatcgg gtcgaagacc aacctgcgct tcgactacga
     5101 tccgcagctg ttgcccacgg gaaagctttc tttccacgtc gtcgatatgc ccgtcagccg
     5161 gctgctggac cagctgttca ggggcaccgg tttgagctac acgctgattg gccggggagt
     5221 ggtccttcgc ggacgcaagg agccggtgaa cattacagtg agcggcttcg tcagggacgg
     5281 gcagacgggg gaatggctca tcggggcaag tatctttctc cccggccacg acgccggcgc
     5341 cgccagcaac gactatggct tttattccct caccgtacct gcggcggata gcctcgaagt
     5401 gggcatttcc tatgttggct accaaaccct gtatcgacgg gtaagtggtc gccatgacct
     5461 ggcccttacc caggacctgg agcgcaatga ggcggaaacg cgcatcgaac cgatgacctt
     5521 gtcgaaagac agacgggaag agaatgttac acggaaccag acatccgtcg tggagctgaa
     5581 cagcggagtg gcaggatcgg cgctctcggt cgggggcagc ggcgacctgg tcaattccct
     5641 gcagctggtg ccgggcgtgc agattggcat cgatggctcc gccggctatt cggtaagggg
     5701 aggcaatgcc ggccagaacc tggtgctgct ggacgaagcg acgatttaca atcccagcca
     5761 tcttttcgga ctggcaggga tcttcccggc ggaggcagtg aagtatgccg agttcctcag
     5821 aggcgggttc ccggcgcaat atggcgacca cctgagctcg gtgcttaaca tcgcgatgaa
     5881 agacggtaac agtcagcagc agggcgcaag tgcgcagatg gggaccatcg tttccggctt
     5941 gcaggtatac ggaccactgt cccgcgacca ctcttcctat ttcgtgtcgg caagacgaag
     6001 cctcatcgac ctttgggcag gctcatttgc cacgaatgat tatttcagca attattattt
     6061 ttttgacatc aacgcaaaat tgaatttccg ggtttcaggc cgcgacaggc tgttgttcag
     6121 cttttaccgg ggccgcgatc agaacggtta ttccggcgaa gcgccggcgg gcggcgggat
     6181 catatacgat acccatttcg gcaacctggc cggaagcatc cgctggaacc acgtgctgtc
     6241 ccccaggtta ttcgtcaaca gctcacttat ttacaaccat tatgagcaat tcctttccgc
     6301 cacgcaggga ggatatttcg cgcaactgta ttccggcatc cgggacaccc ggttcaggag
     6361 cgatctttct ttttatgtcc atcccggtca caggatcagc gccggcatcg atctgctttc
     6421 acaggtgttg tcccccgcat ccctgtcgga taagatcggc gcgggcggcg cgggtatcga
     6481 tgtggccggc ataccgtcca aaaatacggt ccggtctgcc ctttatgccg gagacgagat
     6541 ccacgccggt gaccactggg ttgcctatgc aggtatccgg ggcgcacggt atgatgccga
     6601 tggagtaagc tactttaacg cagagccccg gctttccctg ctttatctgc tcaattcccg
     6661 gaccagcctg aaggcctcct atagccggac cgatcaatac cttcatttgg tgcaaagcta
     6721 caacgcctcc ttcccggccg agatctgggt cgggagcagt ccgctcctgc atccccagac
     6781 ggcagatgaa tacaccgccg gtatctttcg gaatatccac gacaacagta tccaggcaag
     6841 cctggaggtg tattacaaga atatgctgaa ccaatcgctt ttcaaggggg tcacaagccc
     6901 ggtcatcgat aatagccttg agaacaagct gatcttcggc cggggctgga gctatggcgc
     6961 cgaatggatg atccggaaga accgcggccg ctggaagggc tggctttcct attcccttgc
     7021 ctatgcctgg cagcaattcg attccctcaa cggcggtaaa gcattcccct ttgcactgga
     7081 caggagacat agtgtggcca tagcaacaac ctacgagctg aatatgcact ggaaggcttc
     7141 cgccaccttc tatgcagcaa gcggccgggc ctttacactg gcgaccccgg cggcaggcgg
     7201 ccagcagaat ccgaacccct tgtatgagga cgaccatggt gacgatggcg aacaggcccc
     7261 ttccagtcag caggatatca ataactatcg cctgtccccc tataacaggc tggacctcgg
     7321 gcttagctac aaccgcggac atcgcgatca cgcgatcctg aaggacgtga cggtgacgct
     7381 taccgtctac aatgtctacg ccagatacaa cgcggagttt gcctaccgca ccatcgatcc
     7441 cctgactggc aaggcggctg ccacacaaat cacctttatc cccgtgatcc ccagcctgac
     7501 ggtcggcatc accttgtgat cccttgtcat ggcaagcccc gataaataag cccgataaaa
     7561 cgtatattta ctggtcccca aacgcttgcc atatgaagca tttacctgcc acggagcaaa
     7621 ttgcgtttta tcaggccaac ggctttgtcg ttattgaaga tttcctcgcg ccggatgagc
     7681 tcgggcattg gcgcaagact gtcatgcagg cagtcgcgga aagaggagga agaaagatcc
     7741 ccgggaaaga catcctcatc ggcgaagacg acgggatcaa tgaggacggg gattatttcg
     7801 ggaaggtttt tgatcagctg ttgaaccttt ggcagacgaa tactgcggta aaggagttga
     7861 tgttggatga gcggatcggc aggatggccg ccatgctttc cggagcggaa gggatcagga
     7921 tctggcatga ccaggcactc ttcaaacggc cctgggccaa tcccacggca tggcatttgg
     7981 acaacccttt ctggtccttc tacgaccgaa gggcgatctc catctgggta gcgctcgacg
     8041 atgcgacatg gcaaaacggc tgcctgtatt ttattcccgg ctcgcacaag gggacgaact
     8101 ttgacaagat aaccatcggc cggaatatgg acggcatatt cgatatctac ccgcagtatg
     8161 cgaaaacgcc gtcggttgcc gcaccgatga aggccggcag ttgctccttc cacaacggac
     8221 tgaccattca cggagcgggc gccaatatga ccaatgcctg gcggcgggcg atgacctgcg
     8281 cctacatgcc tgacggcaac gtattcaacg gggagccgaa tatcctcccc gaaccctatt
     8341 tgagcagcct gaccaccgga gacctgctcg ataacaatga acagaaccca cttatttatg
     8401 cagcccattg agcgtcccgc tgtgccaccc aggaaaaccg cagcgggata ttcaggccgg
     8461 ttcatccaca cgatctctat cctcgcgttt ctcctactca gcccgcctgc aaatgcgcaa
     8521 ctgaccccgc gcccgaatgc gcaactgacc ccgcgccacc tgctggagaa acagatcgat
     8581 cttgcaggaa tgcaaaaggt gcttttttca aaggcggcat ggaagccgtt cccccggtcg
     8641 gcggcggagt gggaaaagat cttgccagac agcatcagag cggtattgat caggaacggc
     8701 gaggcatgcc tgcaagggtc gttccccaac gtaccggctt ccgtgaccct ggacttcttt
     8761 cgcaacggca accggacaag atacgagagc atcgcttttg gaaaacgaaa ccggctttgg
     8821 gcgctcgtac tggccgaatc cgtggaaggc aggggacggt tcctggatgc gatccttgac
     8881 gggatatggt ccatctcgga agaaaccttt tggggcgcct ccgcacatct ctttttacaa
     8941 aaaggaggaa aggggctgcc tgacgcggag aacccggtgg
//
LOCUS       DATGFG010000323         9695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1073313, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000323 DATGFG010000000
VERSION     DATGFG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073313"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VKQ52_06085"
     CDS             <1..548
                     /locus_tag="VKQ52_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86784.1"
                     /translation="SFKLVPYNSPLTRNQHPFYRKFRAGLVKNGVAEGIYETDSTIIS
                     MNPSTNKYTDVLRYPLVLLTYAEASDMASGGPTAGGYAAINLVRARAGEPPLTAGLSQ
                     IAFRDSVVFERAYEFGAEMGTRWFDILRLQLLPQVVAARSPLDNPISPTNLAILQDKY
                     YAPIPVNEMLKNPQWTQNPGY"
     gene            complement(625..7149)
                     /locus_tag="VKQ52_06090"
     CDS             complement(625..7149)
                     /locus_tag="VKQ52_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HLZ86785.1"
                     /translation="MRSTILLLFVALSFAHLATAQTVQGTAPREKVAPPKEDNKEEGE
                     NESAETALEHAKQKWFRLMQKPNADYFAIQRKFLRYFRKHANEGSGPKEYGASWLKTK
                     LFYLNARGRVQAPPSIDYNRLPKGYPMSVSGVTDTMSGDWRMVGPRNTFTTLALSGTN
                     GGYAYCVRMDPTNPNKLFISFITGGLWVSADNGNSWHLADANMPANAYYDIDVCRAHN
                     STVYAVSSGAVIKSTDGGMSWRATTLNSTNYSGQGYDIAASPTDSNVVVARWGTNLYR
                     TADGGATWSVIRSGLNTTFNIWDCNINGEILDWDNSNDHTVYLTDRPDNQNYVTVYKS
                     TDAGASFSLLQTLTLPSSANGAVTAWSKVCTATNDTSTVYILIGSGANSSSGIAAQLF
                     KLDIATGTILLQRINMISGANTSYGSTNAFVHGDIAMDIHNENNIVWGCYSQTNAQYS
                     TDNGATFSMSDLSVHFDLRTMFMMDGKVILGTDGSVVLSTDSGNHFTIVSNSISNQEL
                     WGFGSAFNSDILAAGCDHGPLAIRDYEAPGGWYQVLGADQGNSDVNPLDSVTVYSNGY
                     DTYHVTRTGIKTFRTSSQQIDPGGIYSYFNTLQFHPNLYHTLITHDAGQYPTSVAQAT
                     RDIWKNSLVRSDDNGLTVKVVHTFQNPLFREKMCMSDTNRIYVVVGLANSKLMKTTDG
                     GAIWTDITPASSVTGAGVTNISDLAVSDVNPNEIWVTYSGVQNTCQVLHSTNGGVSYV
                     NLTTATLTSNPVTRIIFQRGTNGGVYVGNVSGIYYRNDNMPDWVLLGNGLPQMDMRFM
                     FINYSKEKILIGTSRGAWDHDLYEHSSTSAQISASTQRPNCQSPLVQFKDYSVVSNKG
                     KAVTYSWSFPGGNPATSTLQNPLVSYAGDSSGLHSVSLTVTDQYGTSTQTLPNFIYCD
                     GTNCPPFVAPKADTVAGNALDLTPAGDPVIALSPMPIKSNTFTITLWTKPRGLQQVFS
                     QIISSNTPNTLFGIGISYPGNYINNLNLTFTSNSVVFWQQSTLNLAADQWNHVALTYT
                     PDSVSIYLNGGAPWTIPAASSSTPSGFPPIDLSEAPVTVNADIHGQGGNYKGQIDEIC
                     FYNYALSQEDIREKMHLTRAPGRDSGLVGYYQFNQYNPAIDTLYDAMGHGTASAVSPS
                     NISPSTVPVASGVSYGIPNVNAPGVYAFPGTGLALTFPGPVVPNGEIVVSRLNSLPDS
                     LPALYKSVGNQYWVVRNWGANAGFTALTSARFTGNNIDPIDSTRHHVPALFLRSANEY
                     LDNWTPLCSPATPSSGNTDTAVFGSSCNDTSFGQFFIGMIPSSVCSGGSAAGCPAYDS
                     VPGKAINFYDYTYFNVPVSAPATNTFTVTAWVRPKGIQQIQTGILAWDQGYFYLSQNN
                     DNQLEYLWNSPNAGTAWNSGLFVQPDQWSFVAMVVHPDSTTLYLNDHMSTDATPQGVS
                     PVTNALLGSSNPGFGYYFGLMDEVTVWSRALSTNEIDSLRHLTREKMANRTLPGHDTS
                     LIGYFQFNDTLSSRSYNLVDSALFPFSAGAGKIASTAPVGGGVSAVQFINGPGSYTFG
                     NTGVKLNYPATGAYPNGNIRISKLNLLPDQYPAASIQPGCYWVMDNYGADSVFAPIAS
                     VQMDSAIGVPSGNVASPNTFKLFKRPVTAEGYTWGNYITSATAATGGTPGSVTFGTAD
                     ITSEGQLFINGGVQPPGSFQAPQADTVAGKVLDLTPAGDPVIALSPVPINSNTFTITF
                     WTKPRGLQQVFSQMISSNTPNTLFGIGISYPANYIDNLNLTFTSSSIAFWQQSTLNLA
                     ADQWNHVALTYTPDAVSIYLNGGVPWIFPAAYSNTPSGFPPIDLSEAPVTVNADIHNQ
                     GANYKGQMDEICFYNYALSQEEIREKMHLTKVPGRDSGLVGYYQFNQYNATIDTLYDA
                     MGNGTASAVSPSNIGPSTVPVASGVSYSIPNVYTPGAYAFPGTGLALTFPGAVVPNGE
                     IVVSRLNSLPDSLPATYEGVGNQYWVVRNWGANASFTALTSAQFTGNNINPTDGTSHQ
                     TPTLFIRGANEYLDNWTSLCSPATPSSGNTDTAVFGSSCNVTGFGQFFIGKISSLVTS
                     KKGKDSVQNIHSTVVSPNFGGAGFIRITPNPVNGSQAITIQNVSTSEAHVDFYLSDGR
                     LYGKYLIEPGSSIQVSHLPRGAILYNASTNDGQKASGVEIVL"
     gene            7423..7710
                     /locus_tag="VKQ52_06095"
     CDS             7423..7710
                     /locus_tag="VKQ52_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HLZ86786.1"
                     /translation="MSKSFLHIFRVTGMYNSADVAAVRSRLVTIPGVSAVSVDLRKMQ
                     AQITATRNIEALALRNALGNVDFGLSGLTTTVITTQSGVRDDEMEEPARPI"
     gene            8429..8707
                     /locus_tag="VKQ52_06100"
     CDS             8429..8707
                     /locus_tag="VKQ52_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86787.1"
                     /translation="MLNTESTAKAGNSAKTDKADISPLENQKGVENHKKAAEHYQLAA
                     KHHLEAARHHEAGAHDKAAESTVAAHGHSLLAREHQKEDAKDHASKNK"
     gene            8806..>9695
                     /locus_tag="VKQ52_06105"
     CDS             8806..>9695
                     /locus_tag="VKQ52_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HLZ86788.1"
                     /translation="MRSKNVFGGFIFVVILGVGLFAAYAAYDPVERTASIVIGTGAFL
                     LALLVSSAIKIADPWDKAIVLRLGHFKSLRGPGLFLIVPILDTIPYWIDTRVITTSFK
                     AEKTLTKDTVPVDVDAVLFWKVMDPKKTALEVADYLTAISWAAQTALRDVIGKTMLSD
                     MLEGRDKISNALQTIIDERTEPWGIHVNSVEVKDVLIPSALENAMSMQAQAERERQAR
                     VILGDSERQVAEKFGEAALTYANNPVALHLRAMNMLYEGLKQNATIVIVPSSAVETMG
                     LGGMAGISALTMGIGQDKVN"
BASE COUNT         2220 a         2464 c         2713 g         2298 t
ORIGIN      
        1 gcagtttcaa gctcgttccg tataattctc ctttgacccg gaaccagcat cctttttatc
       61 ggaagttcag ggccggtctg gtgaaaaatg gcgttgcgga gggcatctat gaaacggatt
      121 caaccatcat ctccatgaat ccgagcacca acaagtacac agacgtgctc cgttatccgc
      181 tggtgctgct gacctacgca gaggcctccg atatggcgtc cggcggaccg acggccggcg
      241 ggtatgcggc catcaacctt gtccgcgccc gggcaggaga gccgccgctg acagccggtc
      301 tttcccagat cgctttccgg gattccgtcg ttttcgaacg ggcttatgaa ttcggcgccg
      361 aaatgggcac gcggtggttc gatatcctgc ggctccagct tctgccgcag gtggtggccg
      421 ccagaagccc actggataat ccgatcagtc ccaccaacct ggcgatcctc caggataaat
      481 attacgcccc cattccggtg aacgagatgc ttaagaaccc gcaatggaca caaaatcccg
      541 gttactagca gtgtaacaat agcagcaatc aggttaatca tgcccggcat ggttaacctt
      601 ttttttccgc gcaataacgg cttatcataa aacaatctcc acccccgaag ccttttgccc
      661 atcattagta ctcgcgttgt ataaaatagc ccccctgggc aaatgagata cctgaatact
      721 gctccccggc tcaatcaaat attttccata cagcctgccg tcggatagat aaaagtcgac
      781 atgggcttcg gatgtactta cattctgaat ggttatggcc tggctgccat ttacgggatt
      841 gggcgtgatc ctgatgaagc ctgctccgcc aaaattcggg ctcaccactg ttgaatgtat
      901 gttttgcacg ctatccttgc cttttttcga tgtgaccagg gaggatattt taccaatgaa
      961 gaattgaccg aagcccgtaa cattacaact gctgccgaat actgccgtat cggtgttgcc
     1021 ggaagacggc gttgccgggc tgcacaagga ggtccaattg tcaagatact cgttcgcgcc
     1081 ccttatgaag agcgtgggcg tttgatgcga cgtgccatcg gtggggttga tgttgttgcc
     1141 ggtaaattga gcggatgtca gcgctgtgaa gctggcgttc gccccccagt tccggaccac
     1201 ccaatactga ttccctacgc cttcgtaggt tgccggcaga ctgtcgggca gactattgag
     1261 cctgctgacc acgatctctc cgttagggac caccgcaccg gggaaggtca gggcgagacc
     1321 ggtgccgggg aaggcatacg cgcccggggt gtatacattg gggatactgt agctgacccc
     1381 ggaggcgacc ggaaccgtgg aaggaccgat attcgagggg ctgacggcag aggccgttcc
     1441 attgcccatc gcatcataga gcgtgtcgat ggtggcgttg tactggttaa actgatagta
     1501 acccacgagc ccggaatccc ggccaggcac ttttgtgaga tgcatctttt cccggatctc
     1561 ctcctggctg agggcatagt tatagaaaca gatctcgtcc atctggcctt tgtagttggc
     1621 gccctggtta tgaatgtctg cattgacggt aacaggggcc tcggagagat cgatgggcgg
     1681 gaagccggag ggagtgttgc tatacgcggc cgggaagatc cagggcacac ctccgttcag
     1741 atagatactc acggcgtccg gggtataggt aagggcgacg tggttccatt gatcagccgc
     1801 caggttcaac gtgctttgct gccagaacgc gatcgaactg ctggtgaagg tgagattcag
     1861 gttgtcgata taattcgccg ggtaggaaat accgataccg aaaagcgtgt tgggggtgtt
     1921 gctggagatc atttgggaga agacctgctg caggccccgc ggctttgtcc aaaaggtaat
     1981 ggtgaacgta ttgctgttga tcggcacggg agaaagggct atcaccgggt cacccgccgg
     2041 cgttaagtcc aggaccttgc ctgcaacagt gtcggcctgg ggcgcctgga agcttccagg
     2101 cggctggaca ccgccgttta tgaacaattg gccttcactg gtaatatctg ccgtaccaaa
     2161 ggtcacgctg ccaggtgtcc ctcccgtagc agcggtcgcg ctggtgatat aattacccca
     2221 ggtatagcct tctgccgtca cgggtcgttt gaatagtttg aacgtattgg gagacgcgac
     2281 attcccggaa gggacgccga ttgcgctgtc catttgcacc gaggcaatgg gcgcgaacac
     2341 gctatccgct ccataattgt ccatgaccca atagcacccg ggctgaatag atgccgccgg
     2401 atattggtcg ggaagcagat tgagtttgct gatccggata ttcccgttgg gataggcgcc
     2461 ggtggccgga tagttgagtt ttaccccggt gttgccaaaa gtatagctgc cggggccgtt
     2521 gatgaattgc acagcgctaa ccccgccgcc tacgggagcc gtggaggcga ttttgcccgc
     2581 gcccgcgctg aaaggaaaaa gggcgctgtc caccagattg taactccgac tggaaagggt
     2641 gtcattaaac tggaaatagc ctatcagcga agtgtcatgg cccgggaggg ttctattggc
     2701 cattttttcc ctggtgaggt gacgtaagct gtctatttcg ttggtggata aggcccggct
     2761 ccacacggtg acttcatcca tcaggccaaa ataatacccg aagcccgggt ttgaacttcc
     2821 gagcagggca ttggtcaccg gtgaaactcc ctggggtgtg gcatccgtgg acatgtggtc
     2881 gttgaggtac aaggtggtgc tgtccggatg aacgaccatc gctacaaatg accattgatc
     2941 gggctgcaca aaaagcccgc tgttccaggc ggtgccggca ttgggggaat tccagagata
     3001 ctcgagctga ttgtcgttgt tctggctaag ataaaaatag ccctggtccc aggcaaggat
     3061 gccggtttgg atctgctgaa tacccttcgg tcttacccag gcggtgaccg taaaggtatt
     3121 ggtggccgga gcagagacgg ggacattgaa ataggtgtaa tcgtaaaaat taatggcttt
     3181 gccgggtacg gaatcatagg ccgggcaacc ggcggccgat cctccgctgc acacagacga
     3241 ggggatcatg ccaatgaaga attgaccgaa actggtatca ttacaactgc tgccgaatac
     3301 tgccgtatcc gtgttgccgg aagacggggt tgccgggctg cacaaggggg tccaattgtc
     3361 gagatactcg ttcgcgctcc tcaggaagag agcaggcaca tgatgtcttg tgctatcgat
     3421 gggatcgatg ttgttgccgg taaaccgggc ggatgtcagc gcggtgaaac cggcgttcgc
     3481 cccccagttc cggaccaccc aatactgatt tcccacactt ttatacaggg ccggcaggct
     3541 gtcgggcaga ctattgagcc tgctgacgac gatctctccg ttagggacca ccggcccggg
     3601 gaaggtcagg gcgaggcccg tgccggggaa ggcatacaca cccggggcgt ttacattggg
     3661 gatgccataa ctgacaccgg aagcgaccgg aacggtggat gggctgatat tcgaagggct
     3721 gacggcggag gccgttccat ggcccatcgc atcgtagagg gtatcgatgg cgggattgta
     3781 ttggttgaac tgatagtaac ccacgagccc ggaatcccgc ccaggcgccc ttgtcagatg
     3841 catcttttcc cggatatcct cctggctgag ggcatagtta tagaaacaga tctcatcgat
     3901 ctggcccttg tagttgccgc cctgaccatg aatgtctgca ttgacggtaa caggggcctc
     3961 ggagaggtcg atgggcggga agccggaagg agtgctgctg gatgcagccg ggatggtcca
     4021 gggcgcgcct ccattcagat agatactcac agagtccggg gtataggtaa gcgcgacatg
     4081 gttccattga tcagccgcca ggtttaacgt gctttgctgc cagaaaacga ccgaattgct
     4141 ggtgaaggtg agattcaggt tgttaatata gttccccggg tacgaaatac cgatcccgaa
     4201 gagcgtgttg ggggtgttac tggagatgat ttgagagaac acctgctgca ggccccgggg
     4261 cttcgtccaa agggtgatcg tgaaagtatt gcttttgatg ggcatgggcg aaagggctat
     4321 caccgggtca ccagccgggg ttaagtccag tgcgttgcct gcaacagtat cggccttcgg
     4381 cgccacgaag ggagggcaat tagtgccatc gcagtagatg aaattgggga gcgtttgcgt
     4441 gctggtcccg tattgatccg ttacggtgag gctaacgcta tgcaggccgc tggaatcacc
     4501 tgcgtagctg accagcgggt tttgaagggt cgaggtagcc ggatttccgc ctggaaaact
     4561 ccaggaatag gtgaccgcct tccctttgtt gctcaccaca ctgtagtcct tgaattgtac
     4621 caaaggagat tgacaattgg gtctttgcgt actcgccgag atctgtgcac tggtggaaga
     4681 atgttcatac aggtcgtgat cccatgctcc gcgactggtg ccgatcaata tcttctcctt
     4741 actgtaattg atgaacataa accgcatgtc catctggggt aacccattgc ccagcaaaac
     4801 ccaatcgggc atattgtcat ttctgtagta gatcccggag acatttccca cataaacgcc
     4861 gccgttggtg ccgcgttgaa aaatgatcct ggttacgggg ttgctggtca gggtagccgt
     4921 ggtcaggttc acatagctga cgcctccgtt agtggagtgc agcacctggc aggtgttttg
     4981 taccccggag taggtaaccc atatctcatt ggggtttaca tcgctgacag caagatcgct
     5041 gatgttcgtc acgccggcgc cggtaaccga tgaggcgggg gtgatgtcgg tccagatagc
     5101 gcccccgtcg gtagtcttca ttaatttgct gttggccaga cccaccacga catagatgcg
     5161 gttcgtatcg ctcatgcaca tcttttccct gaaaagcggg ttttggaagg tatggaccac
     5221 cttcaccgtc aacccgttgt cgtcgcttct gaccaatgag ttcttccaaa tgtcccttgt
     5281 tgcctgggcg acgctggtgg ggtattgccc cgcatcatgg gtgatcaggg tatgatagag
     5341 gttgggatga aattgcaagg tgttgaaata actgtagatc ccgccggggt cgatctgctg
     5401 ggaactggtg cgaaaggttt tgatcccggt ccttgtgacg tgataggtgt catatccatt
     5461 ggagtagaca gtcacgctgt ccagcgggtt gacatcggag ttaccctggt cagctccaag
     5521 cacctggtac cagccgccgg gcgcttcata atcgcgaatc gccaggggac catggtcaca
     5581 acccgccgca agaatgtccg aattgaatgc cgaaccaaac ccccaaagct cctggttgga
     5641 aatgctgttc gaaacaatgg tgaaatggtt tccactatcc gtagagagaa ctaccgaccc
     5701 gtcggtaccc aatatcactt tgccatccat catgaacatg gttctcagat cgaagtgcac
     5761 agaaaggtcg gacatggaaa acgtagcgcc gttgtctgtg gaatactggg cattcgtttg
     5821 actatagcac ccccagacga tattgttttc attatggata tccatggcga tatcaccgtg
     5881 gacgaaggca ttcgttgagc cataactggt attggcgccc gagatcatat tgatgcgttg
     5941 cagcaaaata gtacccgtgg cgatatccag cttgaatagc tgagcggcta tacccgagga
     6001 tgaattcgca ccactgccga tcaggatata taccgtcgag gtatcgttgg ttgccgtaca
     6061 taccttggac caggcggtaa ccgcgccgtt ggccgacgac ggtaaggtca gcgtttgtaa
     6121 aagcgaaaag gatgcgccgg cgtcggtaga cttatacacg gtgacataat tctgattgtc
     6181 tggccggtcc gtgagataca cggtatggtc gttactgttg tcccaatcga ggatctcgcc
     6241 gttgatgttg cagtcccaga tattgaatgt ggtattgagc cccgatctga taacagacca
     6301 ggtggccccg ccgtcggctg tccggtacag gttggtgccc cacctggcca caactacatt
     6361 gctgtcggtg ggggatgcgg cgatatcata accctgaccg ctataattgg tggagttgag
     6421 ggtggtagcg cgccagctca ttcccccgtc cgtcgatttg atgacggctc cgctgctgac
     6481 cgcgtagacc gtgctgttgt gtgccctgca tacatcgatg tcataataag cgttggcggg
     6541 catattggca tcggccaggt gccatgagtt cccattgtcc gcgcttaccc ataagcctcc
     6601 tgttataaag ctgatgaaga gtttgtttgg gttggtgggg tccatgcgta cgcaataggc
     6661 atatcccccg ttggtgcccg atagcgctaa tgtcgtaaag gtattccggg gacctaccat
     6721 ccgccaatcg cccgacatag tatccgtcac gccggagact gacataggat agcccttggg
     6781 aagcctgttg tagtcgatgg agggcggcgc ctgtacccgg cccctggcgt ttaaatagaa
     6841 aagttttgtc tttagccagc tagcgccgta ttctttggga ccgctgcctt cattggcatg
     6901 ttttctgaaa taccggagga attttctctg gatggcgaaa tagtcggcgt tgggcttttg
     6961 catcagcctg aaccactttt gctttgcgtg ctccagggcc gtttccgcac tttcattttc
     7021 gccttcctct ttattgtctt ctttcggtgg cgccactttt tcccgggggg cggttccctg
     7081 caccgtttga gcggtggcca aatgagcaaa agaaagcgct acgaatagga gaagtatagt
     7141 tgatctcata gggtaaagcg caaaaatata cgctttttcg atcgatggat gcttctaagc
     7201 gttagcgcct atccgctgct ccaactagtt cgatctactc actccgtaac gacatcacgg
     7261 ggcgaaaagc agctttttcc tcatcataat gcagaaggat ttgccgaagg cgtaaccaag
     7321 gtgtgaatta tgtaacaaga taccttaagt gtataaggcc tttctcaaaa tatcggatca
     7381 tctttacccc gcgacgccct gccacaacga aaaaggtaaa ctatgtcaaa atcattccta
     7441 catatattcc gggttaccgg aatgtataac agtgcagatg tcgccgccgt caggagcagg
     7501 ctggtaacca taccgggtgt aagcgccgtc agtgtcgacc tgcgaaagat gcaggcgcag
     7561 atcactgcca ccaggaacat cgaggccctg gcattgagaa atgcgcttgg taacgtggat
     7621 ttcggactgt ccgggcttac aaccaccgtc ataaccactc agtccggcgt aagggacgat
     7681 gagatggagg agcctgcccg gcctatttga tccaaaaggg taaaaagctc cggaagaaat
     7741 aatattttat tctttcccga accccgagcc ccgcaatcgg gaatccgcta ttcctaagat
     7801 ccagcgcccg ctccaactgg ctatcggcaa aagcttcccg gtcataaaaa ctaacccctt
     7861 tacccacttc cccgcttttg atagatggga aaaaaagata tcggcccgga ggatcattgt
     7921 actggttcat atgttcggtt tgtcagtgat tttctctgat cgatccgtca aaaaactgtg
     7981 aattatgtaa cataaccgga tttttacata acaccgatta ctcccggatg gtctaccttc
     8041 gtaaaggttt tttgcaggaa attggagtat caagggccgg ggcactgtcc tggccctttt
     8101 tcggtcgagt cggctataac gatcatgcat ccgcccaacg ataaagtatc cctcaagatc
     8161 cgatctctca atcatgatgt tcgttttatt tttattttag ccgtcccgtt aacaaagttg
     8221 acgcttattc cgctctaaag cattatactc ttcctgaaat aagttacacg attcacacat
     8281 atcaagtggg atacatcaga tctctcctgt tatcccccgg catgtgaatg atgcaacgaa
     8341 gggagagttt cgtgtagcaa aacactggat tatattcctc acttttgcga tgtcgtgaaa
     8401 ctttattcgc gatataaacc ggaaataaat gttaaatacg gaatctacgg ctaaggctgg
     8461 caattcggcg aaaacagaca aggctgatat ctctcctctc gaaaatcaga agggagttga
     8521 aaatcacaaa aaagcggcgg agcactatca gttggccgcc aaacaccatc tcgaggctgc
     8581 ccgccatcat gaggccggcg cccatgataa ggccgctgaa agtacggttg ccgcccatgg
     8641 ccattcttta ctggcccgcg aacaccaaaa ggaagatgca aaggaccatg catcgaaaaa
     8701 taaataggcg cgcttgtacc ctttcgatct gtaccgggga ggcatcatac gcaggtgtga
     8761 cgcttccctt tattattcct ttttcttaaa gtcaggaggt ttatcatgag aagcaaaaat
     8821 gtattcggcg ggttcatatt tgtcgtgatc ttgggggtcg gcttatttgc cgcctatgca
     8881 gcctacgacc ctgtcgaaag gacggccagt atagtcattg ggacaggcgc ctttttattg
     8941 gccttgcttg tttcatccgc catcaagatc gcggatccgt gggacaaggc catcgttttg
     9001 cgtctcggcc atttcaaatc actccgcgga ccgggactgt tcctgatcgt tcccatcctg
     9061 gataccatac cgtactggat cgatacccgg gtgatcacca catcgttcaa ggcggaaaag
     9121 acgcttacaa aggacacggt gccggtggac gtcgacgccg tattattttg gaaagtgatg
     9181 gacccgaaaa agacggctct cgaagttgcg gactacctca ccgcgatcag ctgggctgcc
     9241 caaacggcct tgcgtgatgt gatcggtaag actatgttgt cggacatgct ggaaggcagg
     9301 gataagatca gcaatgcgtt gcagacgatc atcgatgaac gcacagaacc atggggtatt
     9361 catgtcaatt ccgtggaagt gaaagacgtg ctgatcccgt ccgcattgga gaacgccatg
     9421 tccatgcagg ctcaggcgga aagggagcgg caggcccgcg ttattctcgg tgattcagaa
     9481 cgtcaggtgg ccgaaaagtt cggcgaggca gcgctcacct atgccaataa cccggtggcc
     9541 ttgcatttga gagcgatgaa catgctctac gaagggctaa agcagaatgc aaccatcgtc
     9601 atcgtgccga gttccgccgt cgagaccatg ggactaggcg gtatggccgg gatatccgca
     9661 ctgaccatgg gcattggcca ggacaaagta aacag
//
LOCUS       DATGFG010000324         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_266309, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000324 DATGFG010000000
VERSION     DATGFG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266309"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VKQ52_06110"
     CDS             complement(<1..1347)
                     /locus_tag="VKQ52_06110"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HLZ86789.1"
                     /translation="MAEEVKKDEEKSLNFLEEIVEEDLAAGKYKSILTRFPPEPNGYL
                     HIGHSKSIVLNFGLAQKYGGKTNLRFDDTNPVTEDTEYVESIKEDIRWLGFEWANEFY
                     ASDYFEQLYLFAEDLIRKGLAYVDDSTAEEIAKMKGTPTEPGKDSPYRSRPVEENLRL
                     FREMRAGKYRDGDKVLRAKVDMSAPNMQLRDPLMYRIKHAHHHRTGDAWCIYPMYDFA
                     HGQSDSIEQITHSICTLEFVSHRPLYDWFIAKLDIFPSHQYEFARLNLNYTVMSKRKL
                     LQLVNEGHVTGWDDPRMPTISGLRRRGYTPESIRLFAEKIGVQKRDNLIDVSLLEFCV
                     REHLNKIALRRMVVFDPLKVVITNYEQEEEWLSSEDNPEDAATGHRSVPFSRELYIEH
                     EDFMENPPKKFFRLSPGQKVRLKSAYIIQCDEVIKDAAGKITELRGSYLPESKSGGA"
     gene            complement(1491..2144)
                     /locus_tag="VKQ52_06115"
     CDS             complement(1491..2144)
                     /locus_tag="VKQ52_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HLZ86790.1"
                     /translation="MSIDTEAYREISKVLGEKIRLVAVSKTKPVEDILALYKEGQRDF
                     GENYVQELGEKQGRLPADIRWHFIGHLQSNKVKYIIPFVHLIHGVDSMRLLKEIDRQA
                     AKAGRVVDVLLQAHIAREETKFGFDAGELEQVMDTVRRTPEEWAHVRICGLMGMASNT
                     GDEAVVSGEFRALKGLLDRWFPSGGILSMGMSGDYQLAVKEGSTMVRIGSLLFGKRG"
BASE COUNT          511 a          719 c          548 g          614 t
ORIGIN      
        1 cgccccgccg ctcttgctct ccggcagata ggaacctctc agctccgtta tcttccccgc
       61 cgcatccttt atcacttcat cacattgaat gatataagcg ctcttcagcc gcaccttctg
      121 tcccggcgac aggcggaaga atttctttgg cggattctcc ataaaatcct cgtgctcgat
      181 atacaattcc cggctgaacg gcaccgatcg gtggccggtg gcggcatctt ccgggttatc
      241 ttcactggat agccattcct cttcctgttc atagttggtg atcaccacct tcaacggatc
      301 gaagacgacc atccggcgaa gggcgatctt attgagatgt tcacgcacac agaattccag
      361 cagcgagaca tcgatcaggt tgtcccgttt ctggacgccg atcttttccg cgaacagccg
      421 gatactctcg ggtgtatacc ccctgcggcg cagacccgag atcgtcggca tccggggatc
      481 gtcccaaccc gtaacatgcc cctcattcac gagttgcagc agcttccgct tgctcatcac
      541 cgtataattg aggttgagcc gcgcaaattc atattgatgc gaagggaaga tatccagttt
      601 cgcgataaac cagtcgtata acggccggtg cgagacaaat tccagcgtac agatggagtg
      661 cgtgatctgt tcgatggaat cgctctggcc atgcgcaaag tcatacatcg ggtagataca
      721 ccaggcatcc ccggtgcgat ggtgatgagc atgtttgatc cggtacatga gggggtcacg
      781 gagctgcatg ttcggtgcgc tcatgtctac ctttgccctt aacaccttgt ccccatcccg
      841 gtattttcca gcccgcatct cccggaacaa acggagattc tcctccaccg gccggctgcg
      901 ataggggctg tctttcccgg gttcggtggg cgtgcccttc attttggcga tctcctccgc
      961 cgtactgtca tccacatagg ccagtccctt gcggataaga tcttccgcaa acaggtataa
     1021 ttgctcgaaa taatcggaag catagaattc attcgcccat tcgaacccaa gccagcggat
     1081 gtcctccttg atactttcta cgtattcggt atcttccgta acgggattgg tatcgtcaaa
     1141 ccgcagattg gtcttgccgc catacttctg cgcgagcccg aaattgagca caatggattt
     1201 ggagtggccg atatggagat agccgttcgg ctcggggggg aagcgcgtaa ggatgctctt
     1261 gtattttccc gccgccagat cctcttccac gatctcctcc aggaaattga gggatttttc
     1321 ttcgtctttt tttacttctt ctgccataaa aaattccact gcgccatagg tccgtcggaa
     1381 atataatcct atggcgcaga ttttttatct tctatttaat tggccttcgg cccgggcgac
     1441 ggtgtcgccc gccgcgaatt tacgccttcc caccgagcct gccaaggccg ctacccgcgt
     1501 ttaccaaaga ggagactacc gatcctgacc attgtactgc cttcttttac agccaattga
     1561 tagtccccgc tcatccccat cgacaggatg ccgcccgatg ggaaccaccg atcgagcaaa
     1621 ccttttagcg cccggaattc cccgctcacc accgcttcat caccggtgtt cgacgccatg
     1681 cccatcagtc cgcagatgcg cacatgcgcc cactcttccg gcgtgcgacg cacggtgtcc
     1741 atcacctgct cgagttcgcc ggcatcgaac ccgaattttg tttcctcccg cgcaatatgc
     1801 gcctgaagca acacatccac taccctcccc gcttttgcgg cctgccggtc gatctccttc
     1861 aacaaccgca tactgtccac tccatggata agatgtacaa agggaatgat atactttacc
     1921 ttattggact gaagatgacc gataaaatgc cagcggatat cggcgggcag ccggccctgc
     1981 ttctccccca gttcctgtac atagttctcg ccaaaatccc gctgcccttc cttatatagt
     2041 gctaaaatat cttcgacggg tttagtcttt gaaacggcga ccagtcgtat tttttcaccc
     2101 aataccttgc ttatctctct gtatgcttcc gtatctatcg acatgttaaa agggtttaac
     2161 gcgaagatac ctacacgagc ggtaatattc cggattggct tttcggctaa atcgatttac
     2221 tttcgcagag tcatggtcac aaagcattat cactttcccg tcacgaaagt ctatctctac
     2281 gctgtactat tcttacttcc cattgtcagt gtcgcccaga cccgtccgca tcctgccggt
     2341 actgcactta ccggtaaggt gatcgatggg gagagcaaac aacccattca cg
//
LOCUS       DATGFG010000325         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_68722, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000325 DATGFG010000000
VERSION     DATGFG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68722"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VKQ52_06120"
     CDS             complement(<1..412)
                     /locus_tag="VKQ52_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HLZ86791.1"
                     /translation="MDISYIINELGEDRDSYFNAVAPPIIQTSNFRVDTVEELKALFE
                     DEHSGYLYSRGLNPTVDILRKKLAALDGAEETLVFNSGASAIFAGVLANVKAGDHIVA
                     VEKPYTWAQRMFDVILPRFGVTTTYIDGTRIENFE"
     gene            complement(416..1762)
                     /locus_tag="VKQ52_06125"
     CDS             complement(416..1762)
                     /locus_tag="VKQ52_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLZ86792.1"
                     /translation="MGKKQLNLFDLSMIVVSLVIGMGIFRTPVVAATRAGTPAIFFLA
                     WGLGGVIALCGALTYAEIGSRYPVTGGYYKIFSLGYHPSLAFSINCIELVSNAGSTAL
                     VGVVGAEYIGRFFFHPGEVPPLFRGGVTAAAVALFYVINLLGLKMSSRVQNVLTMVKL
                     LLILLLLASLFGTYPLPAPAVMKTAIDPVEPMSFLRTLGFTLVAVSFSYGGYQQTINF
                     GGEVQRPGRTIPRGIGIGIALIISLYLALNFVYVKVIGFHDLQSADSIAAQLMLHLFG
                     PVGDRILRCLMFVSVLAYVNVSVMSNPRVMFAMSGEGILPAVFRQTTAKHGVIVWSLT
                     AFSATIVLSLVFTTAVEKIIDYTIFLDSIAFVFSAGTLFILRRRRMGEDRDIYRMKGF
                     PFIPAFFMLAYASVTTSIAIGDPKSAGYCVLIFLFFFILYFILKSRLRPAGPATEQ"
     gene            1807..>3582
                     /locus_tag="VKQ52_06130"
     CDS             1807..>3582
                     /locus_tag="VKQ52_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86793.1"
                     /translation="MKKNNPLLLILCCCGLTLSAQDVHPSAFLTDSSDTRPLGEVVVR
                     AYEQNRKLAEVGAPVNIISKAALNRFGNIGILPAMNTAPGVRMEERSPGSYRLNIRGS
                     SLRSPFGVRDVKIYYNEIPLTDPGGNTYLNQLGFYTIQSLEIIKGTAGSLYGAGIGGA
                     VLISSMPTTWEKGISADYTAGNYAYDVKHLATNSLNVDLRTGDADHRNNFTYTHQASD
                     GYRQQTQMRRDIASWETLLKASDRQTIHAYMLYSDLYYQTPGGLTLTEYNKNARMARP
                     PAGGQPGAIQAHAAIFQKNFTAGFSNEYHFNAHWQNTSSVYGAYTDFTNPGIRVYEYR
                     QEPHFGGRTAFQYKTDLGIGNLQLNFGAEAQKGFFETRDYINKSGVRDALQTDDKLNN
                     YTWFGFVQGDLRLRDGWTLTAGGSLNKTAVDITRVSVLPPVTHPVRFDNKFAPRIALL
                     KRITPDVSLYASASRGFSTPAVSELEKSNGIVGPPLQPEDGIDYELGSRGSFLNGKLF
                     FDLDAFIFHIRNAIVQRIDSVGVGYSINAGGTDQHGLETFVSYQLIDEPHRFFSNVRV
                     FASDTWHDFHYSSFTQDTSNFSGHRL"
BASE COUNT          863 a         1085 c          976 g          658 t
ORIGIN      
        1 gttcgaagtt ctcgatacgg gtgccgtcga tataggtggt cgtcacgcca aatcgcggga
       61 ggatgacatc gaacatccgc tgcgcccagg tatacggctt ttccacggcc acgatatgat
      121 ccccggcctt tacattggcc aggacaccgg caaagatggc cgaggccccg ctgttgaaga
      181 cgagggtctc ttcggcgccg tccagcgcgg ccagcttctt acggaggatg tcaaccgtcg
      241 gattgagacc gcggctgtag aggtagccgc tgtgttcgtc ctcgaaaagg gcctttagct
      301 cctccacggt atcgaccctg aaattgctgg tctggatgat gggtggggcg acggcgttaa
      361 aataggagtc gcggtcctcg cccaattcgt tgatgatata ggaaatgtcc atatactatt
      421 gctccgtggc cgggcctgcc ggccgcagcc ttgactttaa gatgaaatat aagataaaaa
      481 aaaagaggaa gatcagcaca caatacccgg cagatttagg gtccccgatg gcgatgctgg
      541 tcgtcacact ggcataggcc agcataaaaa aggccgggat gaacgggaac cccttcatcc
      601 ggtaaatgtc ccggtcctcg cccatccgcc gccgccgcag gatgaacagg gtccccgcgg
      661 agaagacaaa ggcgatgctg tccaggaaaa tggtatagtc gatgatcttc tccaccgccg
      721 tcgtgaacac caggctgagc acgatcgtag cggaaaaagc agtgagactc cagacaatga
      781 ccccatgctt tgccgtggtc tgccgaaaga cggcgggaag gataccttca ccgctcatcg
      841 caaacatcac ccgcggattg ctcatcaccg atacgttgac ataggcgagc acggatacga
      901 acatcaggca gcgaaggatc ctgtccccaa cgggcccgaa caggtgcagc atcagttggg
      961 cggcgatgga atcggcagac tgcaggtcat gaaacccgat cacctttaca taaacgaagt
     1021 tgagggcgag atataacgag atgataaggg cgatgccgat accgatgccc cggggaatgg
     1081 tgcggccggg ccgttgcacc tccccgccga agttgatggt ctgctggtag ccgccatagc
     1141 tgaacgaaac cgcgacaagg gtaaagccaa gggtcctgag aaagctcatc ggctctacgg
     1201 ggtcgatggc cgtcttcatc accgccggcg ccggaagcgg atacgtacca aaaagcgagg
     1261 ccagcaacaa caggatcagc aggagcttca ccatcgtcag cacattctgc acccggctac
     1321 tcatcttcag accgagcagg ttgatcacat aaaagagagc cacggcggca gccgttaccc
     1381 cgccgcgaaa aagagggggc acctcaccgg gatgaaagaa gaagcggccg atgtactccg
     1441 ccccgacgac acccaccagc gcggttgatc cggcattgga gaccagttcg atacagttga
     1501 tggaaaaggc cagcgaagga tgatagccca gcgagaagat cttgtagtag ccgccggtca
     1561 cggggtaacg ggagccgatc tccgcatagg tgagcgcgcc gcagagggcg atgacgccac
     1621 ccaatcccca ggcaagaaag aagatggccg gggtgccggc cctggtggcg gcgacgacgg
     1681 gggtccggaa gatccccatg ccgatcacca ggctgacgac gatcatgctc aaatcaaaaa
     1741 gattcaactg ttttttaccc ataagccatt tgaagataac ctaaatttgc gtcacattcc
     1801 catcggatga aaaaaaacaa ccccctgctg cttatcctct gttgctgcgg tcttacccta
     1861 tccgcacagg atgtccatcc gtctgctttc ctgaccgatt cgtccgacac aagacccctt
     1921 ggtgaagtag tggtaagagc atacgaacag aaccggaaac tcgcagaagt gggtgccccc
     1981 gtcaacatca tcagcaaggc cgctctcaac cggttcggca atatcggcat cctgccggcg
     2041 atgaataccg caccgggagt ccggatggaa gaacgctccc ccggcagcta ccggctcaat
     2101 atccgcggca gcagccttcg ttcgccattc ggcgtcagag atgtcaagat ttactacaac
     2161 gagatcccgc tcaccgatcc cggcggcaac acctatctca accaattggg gttctatact
     2221 atccaatcgc tggagatcat caagggcacg gcaggaagcc tgtatggcgc gggcatcggc
     2281 ggcgccgtcc tgatcagcag catgcccacg acatgggaga aagggatcag cgcggactat
     2341 acggcgggca actatgccta cgatgtcaaa caccttgcga caaacagcct gaatgtcgat
     2401 ctccgcaccg gcgacgccga tcatcgcaac aacttcacct atacccacca ggccagcgac
     2461 ggctaccgcc agcagaccca aatgcggcgg gacatcgcct cctgggagac cctgctaaag
     2521 gcaagcgacc ggcagaccat ccacgcgtat atgctgtaca gtgatctcta ttaccagacg
     2581 ccgggtggac tcaccctgac cgaatacaac aagaacgcca ggatggcccg cccacccgcg
     2641 ggtggtcagc ccggagcgat ccaggcccat gccgccatct ttcaaaagaa tttcaccgcg
     2701 ggcttcagca acgagtatca cttcaatgcc cactggcaga ataccagctc cgtttacggg
     2761 gcctacaccg attttaccaa ccccggcatc cgcgtctatg agtaccggca ggagccgcat
     2821 tttggcggca gaacggcctt ccagtacaag accgacctgg gcatcggtaa cctgcagctc
     2881 aactttggcg ccgaggcgca aaaaggattc ttcgagaccc gcgactatat caataagtcg
     2941 ggtgtccggg atgcgctgca aaccgacgat aaattgaata actacacctg gttcggcttt
     3001 gtccagggtg atctccggct gagagatggc tggaccctca ccgcgggggg aagtctcaat
     3061 aaaacagcgg tcgatattac ccgggtctcc gtcctgccgc ccgtgaccca ccctgtccgc
     3121 ttcgacaaca aattcgcacc ccgtatcgcc ctgctgaaaa ggatcacccc cgatgtctcc
     3181 ctttacgcca gcgcctcccg cgggttctcg acccccgccg tctcggagct ggaaaaaagc
     3241 aatggcatcg taggtccgcc cttacagccc gaggacggta tcgattatga attgggcagc
     3301 cgcggcagct tcctcaacgg taaactcttt ttcgatctcg acgcctttat cttccatatc
     3361 cgcaatgcca tcgtccagcg gatcgattcg gtcggggtag gctattcgat caacgccggc
     3421 ggcaccgacc agcatggcct cgagaccttt gtctcctacc agctcatcga cgagccgcat
     3481 cgcttcttca gcaacgtccg cgtctttgcc agcgacacct ggcacgattt ccattacagc
     3541 agttttaccc aggacacgag taatttttcc gggcaccgcc tg
//
LOCUS       DATGFG010000326         6281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_257119, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000326 DATGFG010000000
VERSION     DATGFG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257119"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VKQ52_06135"
     CDS             complement(<1..1207)
                     /locus_tag="VKQ52_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86794.1"
                     /translation="MRRLIKIIQFVLVLLSLREGLMAQPVYTIQADSVKLTGCDSSEL
                     IIENHTQNVPGFLFNTGNGRTIFRRGLQSLGNGSFIFGADTLNLSANAWVQGGNRFGA
                     TGVLGTLDNNHLDLYTNNTQYARLTNTGNFLLGSTTDGGARLQVNGGGTFNGALFLNP
                     LTSSDGAGGYNFISMNNQDLIAGFGHSMIFQVPSTATNYYFQFGAGPNLGTPGKALIF
                     SGNNDGNIVVTAPSNYIDLYGVQNVGDDASGLYLTSGGYSGMDGVVVQRNSRSILMTD
                     ALFTVRENSDTTVFEALKNGNIGVGINAPTAQLHTTGTVRFAGLSSDNTQTRVVVGDA
                     NGNLFYRDASTLAASEALRSSLAVNGPVTAKKLTLTAKDWPDYVFDSSYRLPGLAGVG
                     QYIRQQHHLP"
     gene            complement(1213..2853)
                     /locus_tag="VKQ52_06140"
     CDS             complement(1213..2853)
                     /locus_tag="VKQ52_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86795.1"
                     /translation="MRKASKYWLSILVLLFLGEGLAAQGTYTIQADSVKLIGCDSSEL
                     IIENHTQNVPGFLFNTGNGRTIFRRGAQLLGNGYYLIGADTLKLNPNAWVQGGNSFGA
                     TGVLGTLDSNHLDFYTNNTFQARLTNTGSLLLGSTAEGPWKLHVNGGQLLSGQLWQDN
                     GYVTSLGNGLVLDNAIGSRIFSYNKLVYQSDATGDEHLFRNDIGGTFTGNLVTIDPGT
                     AGLNASQLALKVLGTGSQPGLCVNMQGYTGIGTLLPVMPLDVNGDVLIRSGNLRLGGS
                     GWAGITQTCLATHLYNACGSAGFITLGFNTTSGVASGIPDPITVSQFVNCVGISTNSP
                     QAKLHVTGAGTTTSTSSFLVYNGGGNELFRITDTGYIGIGTYHPTAQLHTSGTVRFAG
                     LGSDNTQTRVLVGDANGNLFYRDASTLAANDVIRGSLAVNGAIRAKELTLTARDWPDY
                     VFDSSYLLMPLSGVKEYIHQNAHLPGVAAASAVEADGVDVGNMQAVLLKKIEELTLYT
                     IKQDEEIKTLKEDQGRELAALKEEVTALRQLLLTKPGK"
     gene            complement(2859..4595)
                     /locus_tag="VKQ52_06145"
     CDS             complement(2859..4595)
                     /locus_tag="VKQ52_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86796.1"
                     /translation="MRKVKIIPLLLFVSLFLHDRMAAQGTYTITADSVKLTGCDSSEL
                     IIQNHTQNVPGFLFNTGNGRTVFRRGAQKLNDSLYLIGADTLKTSSHAWQQGGNAFGA
                     TGILGTLDNNHLDLYTNGIKRGRLDSIGQFNLDFANGIGYANAFALTNTNGTVPWVNI
                     AANAQYCVMSLRPNNSAFFGPTFQLTTGYSTTMEAFAYNDLSVKGYYGLDLYGGYGGG
                     ASKAIRFFPGFSTSPTAAFSLNGNFLDGDPGTDNGSRLQVTGTSTFSDNVTIGNNKTL
                     AVGGTYIVDDPNGRYITSVNPESMTYTAGPGGFATFDGNVNLGCGQGNSIYLRPGGAV
                     NRAITISASTGAPSNTTFSFGNLRNDGPKKAVINTADMDGSAYGGTTAVDLYLYPGKE
                     TYSNSQANMILAFDGTAQRGNIGVGTASPTAQLHTTGTVRFAGLSSDNTQTRVVVGDA
                     NGNLFYRDASTLAASEALRSSLAVNGPITAKKLTLDAKAWPDYVFDSAYRLPGLVELK
                     AYLGRQHHLPGLPAAGEVEKNGVDVGAAQAMLLKKIEELTLYAIKQDEEMAAMKKEMA
                     ELRRLIMEKAGK"
     gene            complement(4592..>6281)
                     /locus_tag="VKQ52_06150"
     CDS             complement(4592..>6281)
                     /locus_tag="VKQ52_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86797.1"
                     /translation="AWVQGGNAFGATGILGTLDNNHLDLYANNLQQARLTNTGNFLLG
                     TTTDQGYKLQINGRAFAGGGVLIQNEGSLNTTQNGLVINATGATNYAGADFYNDRSRL
                     GQVFCTGSTYPGFSSIPQQSFGLYTNAPGGLTMVSEDSANAVIRFANGYYGAATEKMR
                     LSGAGNLLLATATDNGNTLQVNGTSYFNGPQQITGTLNAGSNGPFSLFNPVVNAPAGD
                     WYYFFGTRIAPVMNIAGNYQQSYAMDISPTFNLNGYVQHTDAISPALHIGTNLGGIMI
                     EQGTSYPGNSGQPLFINQGGGTDKEAIYNYRNSASTVRPFIWDNDNRPASFSAAIVPA
                     LRSTISNPQAGGGVSFTLDRYYYGTEASIDMRYETTPDSTAHENTSIVFSTRSGGYNV
                     TPLYINGINIGLGTNTPTAQLHTTGAVRFAGLGSDNTQTRVLVGDANGNLFYRDASTL
                     AATEALRSSLAVNGPITAQKLTLTTKDWPDYVFDSSYSLMPLPELKEYVRRNNHLPGI
                     TPAESAEKTGVDVGDTEAKMLKKIEELTLYVIRQDEEIAELKQMIKAKSHKKPK"
BASE COUNT         1392 a         1803 c         1618 g         1468 t
ORIGIN      
        1 cgggcagatg atgctgctgc cggatatatt gcccgacacc cgcaaggccg ggtagccggt
       61 agctgctgtc gaatacatag tccggccagt cttttgccgt caatgtcagt ttctttgcag
      121 tgaccggacc attgacggcc agggacgaac gaagggcttc actcgccgcc agggtcgaag
      181 catcccggta gaaaagattg ccgttcgcgt cgccgaccac gaccctcgtt tgcgtattgt
      241 cggaggagag tccggcaaag cggacggtgc ccgtggtatg gagctgcgcg gtaggggcat
      301 tgatgccgac gcctatgttg ccatttttca acgcctcgaa gacggtagta tcactgtttt
      361 ccctcaccgt gaataacgca tcggtcatca ggatactccg gctattacgc tgtacgacca
      421 cgccgtccat gcctgaatat cccccgctcg tgagatataa acctgatgca tcgtcgccta
      481 cattttgcac gccatacagg tcgatataat tgctgggcgc cgtgacgacg atattcccat
      541 cgttattacc actgaatata agtgctttgc ctggtgtccc gaggttggga ccggcaccaa
      601 actggaagta atagttcgta gcagtgctgg gcacctgaaa tatcatacta tgtccaaatc
      661 cggcgatcag gtcctggttg ttcatggaaa tgaaattgta tccgccggcg ccatcggaag
      721 aagtcagggg attcaaaaac aacgcgccgt tgaacgtgcc gccaccattc acctgcaatc
      781 tcgctccacc gtcggtggtg cttcccaaca ggaaattccc tgtattcgtc agccgggcat
      841 attgcgtgtt gttggtatag agatccagat gattgttgtc cagcgttccc agcaccccgg
      901 tggcgccaaa gcgattaccc ccctgcaccc aggcattggc gctcaggttg agagtatccg
      961 caccaaaaat aaaagagccg ttgcccaacg actgcaagcc ccgcctgaag atcgtacgtc
     1021 cattgccggt attgaataga aaacccggaa cattctgggt atggttctcg atgatcagct
     1081 ccgaggagtc acagcccgtc agcttgacgc tatcggcctg gatcgtatat actggctgcg
     1141 ccatcagccc ttctctcagc gaaaggagca caagaacaaa ctgaattatt ttaatcaatc
     1201 gtctcatatc acttattttc ccggttttgt caataacaat tgccggagtg ccgtcacctc
     1261 ctctttcaac gccgccaact cgcgaccctg atcttccttc agggtcttta tctcctcatc
     1321 ctgttttatg gtatagagcg tgagttcttc aatcttcttc agcaacaccg cctgcatgtt
     1381 acccacatcg accccatctg cttcgacagc cgatgcggca gcgacaccgg gcaagtgggc
     1441 gttctgatgg atatattcct ttacccccga aagcggcata agcaggtagc tgctgtcaaa
     1501 cacatagtcg ggccaatccc ttgccgtcag cgtcagctcc tttgcgcgga tcgcaccatt
     1561 gacagccaat gaaccccgga tgacatcatt cgccgccagc gtcgaggcat cccggtaaaa
     1621 aagattgccg ttggcatcgc ctaccagcac ccgcgtctgc gtgttgtcgg aacccagacc
     1681 ggcgaagcgg accgtcccgg aggtatgcaa ttgggccgta gggtggtagg ttccgatgcc
     1741 gatataaccg gtgtccgtga tcctgaagag ttcgttcccg cccccgttgt atacaaggaa
     1801 agaagaggtg ctggtggtcg tcccggcgcc ggtgacgtgc agcttcgcct ggggactgtt
     1861 ggtgctgatc ccgacgcaat taacaaactg ggaaaccgtg atcggatcgg ggatgccgga
     1921 agcgaccccc gaggtggtat tgaaaccaag cgtaataaat cccgccgacc cgcaggcgtt
     1981 gtacagatga gtggccaggc aggtctgcgt gatgccggcc cagccgcttc cccccaaccg
     2041 cagattcccg ctgcggataa ggacatcccc gtttacatcc aatggcatca cgggtaaaag
     2101 ggtgccgatc ccggtatacc cttgcatgtt cacgcaaagc cctggttggc ttcccgtccc
     2161 caataccttt aatgcgagct gagacgcgtt cagaccggct gttccgggat ctatcgtcac
     2221 caggttgccg gtaaaagtcc cccctatatc gttcctgaaa aggtgttcgt cgcccgttgc
     2281 gtccgactga tataccaact tattgtaaga gaagatccgg gaaccgatag cattgtccag
     2341 gaccaggccg ttacctaacg aagtgacgta cccgttatcc tgccagagct gcccggacag
     2401 caactgtccg ccatttacgt gtaatttcca tggaccctcg gcagtggaac caagcaacag
     2461 gctaccggta ttggtcagcc gggcctgaaa cgtattgttc gtataaaaat cgagatgatt
     2521 actatccagc gttcccagca ccccggtggc gccaaagctg ttaccaccct ggacccacgc
     2581 attggggttg agcttgaggg tatccgcccc gataagataa tatccatttc ccagcaactg
     2641 cgctcccctc ctgaagatcg tacgcccatt gccggtattg aatagaaaac ccggaacatt
     2701 ctgggtgtga ttttcgatga tcagctccga agaatcacaa ccgatgagtt tcacgctgtc
     2761 cgcctggatg gtataggtgc cctgcgcggc aagcccctcg cccaaaaaga gcaggaccag
     2821 gatactcaac caatatttcg atgctttcct catattgtct atttaccggc tttttccatg
     2881 atcaatcgac gcaactccgc catctctttt ttcattgccg ccatctcctc atcctgcttt
     2941 atcgcataaa gggtaagctc ctctatcttc ttcaacaaca tggcttgcgc cgcccccaca
     3001 tcgacgccat tcttttccac ctcgccggcc gcaggaagcc ccggcaaatg atgctgccgc
     3061 cccagatagg ctttcaactc caccaacccg ggtagccggt aggcgctgtc gaatacatag
     3121 tccggccagg cctttgcatc aagcgtcagt tttttcgcag tgatcggccc attaacggcc
     3181 aacgatgagc gaagggcttc actcgccgcc agagtcgaag catcccggta gaagagatta
     3241 ccgttcgcat cgccgaccac gaccctcgtt tgcgtattgt cggaggagag tccggcaaac
     3301 cggaccgtgc ccgtggtatg aagctgcgcg gtcgggctcg cggtgccgac accgatattc
     3361 ccacgctgcg ccgtgccgtc gaacgccagg atcatattcg cctggctgtt cgaataggtc
     3421 tcctttcccg gatacaagta gaggtcgacg gcagttgttc cgccatatgc cgacccgtcc
     3481 atatccgccg tattgatcac ggccttcttg ggaccgtcat tccgcaggtt cccgaacgag
     3541 aaggtggtat tgctcggggc gcccgtcgac gcgctgatgg taatagcacg gttaactgcc
     3601 ccacccgggc ggagatagat actgtttccc tggccgcacc ccaggttgac gtttccgtca
     3661 aaagttgcaa atcctcccgg accagcggtg taggtcatgg attcgggatt cacgctggtg
     3721 atgtatcgcc cattcgggtc atcgacgata taggttccgc ccaccgccag ggtcttattg
     3781 tttccaatag tcacattgtc cgaaaaggtg gatgtccccg tcacctgcag gcgactgccg
     3841 ttatccgtgc ccggatcgcc atcaagaaaa ttgccattca gggaaaaggc cgccgtgggc
     3901 gacgtgctaa accccgggaa gaaacgaatg gcctttgacg ccccgccgcc atagccgcca
     3961 tataggtcca atccataata tcccttcacc gaaagatcat tgtaggcgaa ggcttccatc
     4021 gtcgtggaat agcccgtggt aagttgaaac gtcggcccga aaaaggcaga gttgtttggc
     4081 cggagactca ttacacagta ttgtgcgttc gccgcaatat tgacccaggg aacagtacca
     4141 ttggtattcg taagcgcaaa ggcattggca tagcctatcc cgttggcaaa gtccaggttg
     4201 aactgaccga tgctatccag ccgccccctt tttatcccat tggtataaag atcaagatga
     4261 ttattatcca gcgtccccag gatacccgtg gcgccaaagg cattcccgcc ctgctgccag
     4321 gcatgcgatg aggttttaag ggtgtccgcg ccgatgagat acaggctgtc attcagcttt
     4381 tgcgcacccc gcctgaaaac ggtacgccca ttaccggtat tgaagaggaa ccccggaaca
     4441 ttctgcgtat ggttctggat gatcagctcc gacgaatcac aaccggtgag tttcacgctg
     4501 tcggcagtaa tagtataagt gccctgcgcg gccatccggt catgcaggaa aagcgagaca
     4561 aacaggagca ggggtatgat ctttaccttt ctcattttgg cttcttatgg gatttcgctt
     4621 ttatcatttg cttgagctcc gctatctcct catcctgcct gatcacataa agcgtcagct
     4681 cctctatctt ctttagcatc ttcgcctccg tatcacctac atcgacaccg gtcttttccg
     4741 cactttccgc gggagtaatc cccggcagat ggttgttcct gcggacatat tcctttagct
     4801 cgggaagcgg catcaacgaa tagctgctgt cgaatacata gtccggccag tcttttgtcg
     4861 tcagcgtcag cttttgggcg gtgatgggcc cgttaacggc cagcgatgag cgaagggctt
     4921 cagtcgccgc cagggtcgaa gcgtcccggt agaagagatt gccattcgcg tcgcctacca
     4981 gcacccgcgt ctgcgtattg tccgagccaa gccctgcaaa ccggacggcg ccggtggtat
     5041 gcaactgtgc cgtgggcgtg tttgtgccca gaccgatatt gatcccgtta atataaaggg
     5101 gggtcacgtt atatcccccg ctcctggtag aaaagacgat ggaggtgttc tcatgagcag
     5161 tggagtcagg tgtggtttca taacgcatat cgatcgatgc ctcagtgccg tagtagtacc
     5221 tgtccagcgt gaaagaaacg ccgccgcctg cctgcggatt tgaaatagtg cttctcaatg
     5281 ccggaacgat cgcagcggaa aaagatgccg ggcgattgtc gttatcccat ataaaaggcc
     5341 gtaccgttga ggcgctattg cggtaattat agatggcttc tttatccgta cctccgccct
     5401 ggttaataaa caatggctgg ccggagttac cgggatagga cgtaccctgt tcgatcatta
     5461 tcccgcccag attggtaccg atatgcaatg cgggtgaaat ggcatcggta tgctggacat
     5521 agccattcaa attaaaggtt ggggatatat ccattgcata actttgttga taattccccg
     5581 ctatattcat aactggcgct atccgggtcc caaagaaata ataccagtcg cctgccgggg
     5641 cgttaacgac agggttgaat agggaaaatg gaccgttgct gccggcattc agcgtacccg
     5701 ttatctgctg tggcccgttg aaatagctgg ttccattcac ctgcagggta ttgccattgt
     5761 ccgtcgccgt tgccagaagg agattcccgg cccccgaaag ccgcattttc tccgtcgccg
     5821 cgccataata cccgtttgcg aaccggatga cagcattggc cgagtcttcc gacaccatgg
     5881 ttagcccacc gggcgcattg gtataaaggc caaagctctg ttgaggaatg ctcgaaaaac
     5941 cggggtatgt actcccggtg cagaacacct gcccaagcct ggaacggtca ttataaaaat
     6001 cggcccccgc atagttggtt gccccggttg cgttgatcac gaggccattc tgggtagtat
     6061 tcaacgaccc ttcattctgg atcagcacgc cgccgccggc aaaggcccgc ccgtttattt
     6121 gtaatttata tccctgatcg gtagtcgtcc caagaagaaa atttccggta ttcgtcagcc
     6181 tggcctgttg caggttattc gcataaagat cgaggtgatt attatccaat gtccccaata
     6241 tacccgtcgc tccaaaggca ttacccccct gcacccaggc a
//
LOCUS       DATGFG010000327         4318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_256712, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000327 DATGFG010000000
VERSION     DATGFG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256712"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VKQ52_06155"
     CDS             <1..1068
                     /locus_tag="VKQ52_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ86798.1"
                     /translation="VCSVIIEKELYLIRHTDTGVNRENVVLVPFGTTLPHYAAFKRKV
                     ESMPGVQEAATAHYSMYAGYDMWSAQVPGSGKQMMVRAMDVDNHFVHLLGLQWKSKPP
                     ETLYDGQHVLLNEKAVEKLGLTGDVVGQKLVLGPGEYEVGGVLKDFNYQSLRSGIGEL
                     CLLVEKDTARRWGSTESGYLFVKVNAHVNLPTLIESIRSAYRQFDRDKAFEYRFLDEA
                     FNNTYQAEDRLAGLFGLFTGITVLIACLGLFALATFAAQQRTREIGIRKVLGASVGSL
                     GALLSVDFLRPVVLSTLIASPLAWWFMRRWLEKFAYRTVISWWIFPLAAAALLGIALA
                     TVLFRSLRAARGNPVENLRVQ"
     gene            1233..1535
                     /locus_tag="VKQ52_06160"
     CDS             1233..1535
                     /locus_tag="VKQ52_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HLZ86799.1"
                     /translation="MKAQQVMIGVLSGLVAGVALGILVAPAEGKETRQRIADTADSLK
                     RKLRQLRGVTADELDELKDIFERETEGLKDDVRTRVLELIKAAKEKGNHVKEHALS"
     gene            1821..2462
                     /locus_tag="VKQ52_06165"
     CDS             1821..2462
                     /locus_tag="VKQ52_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86800.1"
                     /translation="MTELLNQTLYVVCGTAWTIAYTFIIIRGFRDKTYGVPFVALAFN
                     ISWEILFSTIFRDGSLVHQVVNSVWAVLDVIILYTYFRYGKRDWPRRVSVKFFYPYSI
                     FVLVVVFALIYFATRELHDTTGAYMAYLQNLMMSLLFINMLNSRGNLSGQSTGIALAK
                     LLGTLTSTVLFYLWRVKFIVFTGGLIFTFDLIYLLLVLNEGRLVWPFRLKLRS"
     gene            2472..3491
                     /locus_tag="VKQ52_06170"
     CDS             2472..3491
                     /locus_tag="VKQ52_06170"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HLZ86801.1"
                     /translation="MPIDLRSDTVTRPTPPMLDAMFSARVGDDVFGEDPSINALEAMA
                     ARLFGMEAALYCPSGTMSNQIAIKCHTQPGDEVICEKNAHVYIYEGGGIAFNSGASVR
                     ALEGDRGRISAEQVSLAINPDDVHRPRTSLVTLENTCNRGGGSCYDFGEIRKIRQVCT
                     DNQLKFHLDGARLFNALVATGRTAKEYGEVFDSISVCLNKGMGCPIGSILLGPSGFIR
                     QARRVRKVFGGGMRQAGFMAAAGIYALENNIQRLEVDHAHARRIAAALEKKDFVRHIM
                     PVETNIVIFETGGAVDCARSFSAQLKEKGILAIAIAPNQIRFVTHLDITPGMVEEVVE
                     VIEQL"
     gene            3527..>4318
                     /locus_tag="VKQ52_06175"
     CDS             3527..>4318
                     /locus_tag="VKQ52_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86802.1"
                     /translation="MFPKVSPKDTKAWQELQNHYDKEMKGVHMRQLFAAEPGRFEHHS
                     LRLDDILFDYSKNIFNEKTLELLRRLAVESKVKEAAEAMFTGEKINATENRSVLHTAL
                     RNLPGTPVLSDGKDVMPDVFRVLGQMKDFSGRIHSGEWKGYTGKKIKYIVNIGIGGSD
                     LGPVMVTEALRPYWKEGIQAYFVSNVDATHIVETLKKVTPEETLFLVASKTFTTQETM
                     TNAHSARDWFLKTARDEKHVAKHFAALSTNEKEVTKFGIDKANMFE"
BASE COUNT          916 a          977 c         1370 g         1055 t
ORIGIN      
        1 gtttgcagcg tcatcatcga aaaggaactc tatttgatcc ggcataccga tacgggagtg
       61 aaccgggaga atgtagtact ggtgcccttt ggaacgacac tgccccacta tgcggcattc
      121 aaacgcaagg tcgagtcgat gccgggggtg caggaggcgg ctactgcgca ctattcgatg
      181 tatgccgggt atgatatgtg gtcggcgcag gttccgggat cgggcaagca gatgatggtg
      241 cgggcgatgg atgtcgacaa ccattttgtc cacctgctgg ggctgcaatg gaagtcgaag
      301 ccccctgaaa cgttgtatga cgggcagcat gtccttctga atgaaaaagc cgtggagaag
      361 ctgggattga cgggggatgt cgtcggacaa aagctcgtcc tcgggccggg agaatatgaa
      421 gtgggcgggg tgctgaagga tttcaattac cagtcgttgc ggagcggcat cggcgagttg
      481 tgcctgttgg tggaaaagga tacggcccgg cgctggggga gtacggagag cggctatctg
      541 ttcgtgaagg tgaatgcgca tgtgaacctg ccgacgctga tcgaatcgat ccgatcggcg
      601 tatcgccagt tcgatcgtga caaggccttc gaatatcgtt ttttggatga ggcattcaac
      661 aatacctatc aggcggagga tcgcctggcg gggttgttcg ggctgtttac ggggatcacg
      721 gtcctgatcg cctgcctggg gctttttgcg ctggcgacct ttgccgcaca gcagcggacg
      781 agggagatcg gtatccggaa ggtgctcggc gcttcggtcg ggtcgctggg ggcgttgttg
      841 agcgtggact ttctgcgtcc cgtggttctg tccacgctga tcgcctcgcc cctggcctgg
      901 tggttcatgc gacgctggct ggagaagttt gcgtaccgga ccgttatctc ctggtggatc
      961 ttccctcttg cggcggcggc tctgttgggt atcgcgttgg cgaccgttct ttttcgcagc
     1021 ctgcgggcgg cgcggggtaa tcctgtcgaa aatcttcgtg tccagtagag tttttagaat
     1081 tttgatgaag agatcggcga cgtggggaaa aaattgctca gtctcccggc tccgggggcg
     1141 gtttgccggc tttaactatt ttcggcattg ttcttggatc aatttgatta catctactcg
     1201 tacattgtct cactgcaaaa aatattattg ttatgaaagc acaacaagtt atgattggcg
     1261 tattatcggg gcttgtcgcc ggtgtagccc tcgggatcct ggtcgcccct gccgaaggga
     1321 aggaaacccg tcagcggatc gccgatacgg cggattcttt gaagcggaag ctgcggcagt
     1381 tgaggggggt tacggccgat gaactggatg agctgaagga tatcttcgag cgggaaacgg
     1441 agggattgaa ggatgatgtc cgtacgcggg tgctggaact gataaaagcc gccaaggaaa
     1501 aaggtaatca cgttaaggaa catgcattgt cctagggagc cccgtaaggc tctatcgcat
     1561 cggagtcccg taaggctcca ggagtttgaa gtcccgtaag gcttcgcagg tggacccgga
     1621 agttttcggg cccacttttt ttatccccgg aactgggtaa tttttggtcg gggcaatagt
     1681 aaaaatacga tgcttttcaa gccattagga gtgataatgg cgtttggggg catttttgtc
     1741 cgtttgattc aggtagaaac acttagtgaa tttagagcaa atattgtact ttcgggcaaa
     1801 tctcttcccc ctctctcccc atgaccgaac tgctaaatca gacgctgtat gtagtgtgcg
     1861 ggaccgcatg gaccatcgcc tataccttta tcattattcg cggctttagg gataagactt
     1921 atggggtgcc ttttgtagca cttgctttca acatttcctg ggagatcctt ttttctacta
     1981 ttttcaggga cggcagtctg gtgcatcagg tggtgaacag cgtctgggcg gtgctggatg
     2041 ttattatcct gtatacgtat tttcgttatg gcaagcggga ctggccgcgc cgggtgagtg
     2101 taaaattctt ttatccctat tcgatctttg tgctggtggt tgttttcgcg ctgatctatt
     2161 ttgcgacgcg ggagctgcat gataccacgg gcgcctatat ggcctatctt cagaatctga
     2221 tgatgtccct tcttttcatc aatatgctca atagccgggg taatctttcg gggcaatcga
     2281 ccgggatcgc gctggcgaag ctgttgggca cgctgacttc caccgtattg ttctatctct
     2341 ggagggtgaa gttcatcgtt tttaccgggg gacttatctt taccttcgat ctcatctatc
     2401 tgctgctggt tctcaatgag gggcgattgg tttggccatt tcgccttaaa ttgcgatcct
     2461 aatcagcaat tatgcccatc gatcttcgtt cggataccgt tacacgccca acgcccccga
     2521 tgctggacgc catgttcagc gcaagagtag gggatgatgt ttttggggaa gacccttcta
     2581 tcaatgcact ggaagccatg gccgcccggc tcttcgggat ggaggcggcc ctttattgtc
     2641 caagcgggac gatgtccaac cagattgcga taaagtgtca tacgcagccg ggggatgagg
     2701 ttatctgcga gaagaatgcg catgtatata tttacgaggg gggtggcatc gcgttcaatt
     2761 cgggggcgag tgtccgggcg ctggaaggtg accgcggccg gatctcggct gagcaggtgt
     2821 cgcttgcgat caacccggac gatgtgcacc ggccgcggac atcccttgtt acgctggaga
     2881 acacctgtaa ccggggtgga gggagttgtt atgacttcgg ggagatcagg aagatccggc
     2941 aggtgtgtac ggacaaccag ctgaagttcc acctggatgg ggcgcggctt tttaacgcgc
     3001 tggtggcgac ggggcggacg gcgaaggaat acggcgaggt tttcgacagt atttccgtgt
     3061 gtttgaacaa gggaatgggt tgcccgatcg ggagtatcct gcttgggccc tcgggcttta
     3121 tccggcaggc ccgcagggtg cggaaggttt ttggcggcgg gatgcggcag gcggggttta
     3181 tggcggccgc ggggatctat gccctggaaa ataatatcca acggctggaa gtggatcatg
     3241 cgcatgcccg gcggatcgcc gcggcgctgg agaagaagga ttttgtacgg cacattatgc
     3301 ccgtggagac caatatcgtc attttcgaga cgggtggcgc cgtggactgt gcgaggagct
     3361 tttccgcgca gctgaaggaa aaaggcatcc ttgccattgc gatcgcgcct aatcagatac
     3421 gattcgtcac gcacctggat atcacgcccg ggatggtcga agaggtagtc gaggttatcg
     3481 aacaattata ggatcgcatt gttaaccacc aaaaaatagt ttcgttatgt tcccgaaagt
     3541 atcacccaaa gacacgaaag cctggcagga gctgcaaaac cattatgaca aggagatgaa
     3601 gggcgtgcat atgcggcaat tgtttgcggc cgagcccggc aggttcgaac atcattcgct
     3661 ccggctggat gacattcttt tcgattactc caagaacatc tttaatgaga agacgctcga
     3721 gctgttgcgc cggctggcgg tggagagcaa ggtaaaggag gcggcggagg cgatgttcac
     3781 gggtgaaaag atcaatgcaa cggagaaccg gagtgtgctg catacggcgc tgcgcaatct
     3841 tccagggacg ccggtgctga gcgacggaaa ggatgtcatg ccggatgtgt tccgggtgtt
     3901 ggggcagatg aaggattttt ccggccgcat tcatagcggg gaatggaagg ggtatacggg
     3961 gaagaagata aaatatatcg tcaatatcgg catcggcggc agtgatctgg gtcccgtgat
     4021 ggtgacggaa gcgttgcggc cttactggaa ggaggggata caggcttatt tcgtgagcaa
     4081 tgtcgatgct acgcatatcg tggagacgtt gaagaaggtg acgccggagg aaacgctttt
     4141 ccttgtggct tccaagacct ttaccacgca ggagacgatg accaatgcgc attcggcgcg
     4201 ggactggttc ctgaagacgg ccagggatga gaagcatgtc gccaaacatt ttgccgcgtt
     4261 gtcgacgaat gagaaggaag tgacgaagtt cgggatcgac aaggcgaata tgttcgag
//
LOCUS       DATGFG010000328         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_508115, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000328 DATGFG010000000
VERSION     DATGFG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508115"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /gene="ppsA"
                     /locus_tag="VKQ52_06180"
     CDS             complement(<1..1856)
                     /gene="ppsA"
                     /locus_tag="VKQ52_06180"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HLZ86803.1"
                     /translation="MSNRIILPLESVGLSDLALVGGKCASLGEMLRNLTGLGVNIPGG
                     FVVSTDAYWQFIEQSQLGPSIRQQLNSIDFDNVESLRRVGQKIRQAISNARFPQELSH
                     QIIAAYEQLSQRYGQSSTDVAVRSSATAEDLPDASFAGQQETFLNVRGPAALIDAVRN
                     CFASLFTDRAISYRKTFNFDVSTVGLSVCVQKMVRSDLASSGVAFSLDTESGFREVVM
                     INGAFGLGEMVVQGALSPDEFLVFKPTRQQGFPSIIEKRLGVKDKMMVYGDHSDERTR
                     VIPVEPWQQHRFCLDDKHILELADLVMTIEKYYSDLKGKWVPVDVEWAVDGLSGELFI
                     VQARPETIHSRKPKNQLIEYRIGGDAVSNKIVVKGIAVGDGIASGKVHIMYSLDKRVG
                     GSDFQEGDVLVTDMTDPDWEPIMKKAAAIITNKGGRTCHAAIVAREMGVPAIVGCGSA
                     TELLSNGMPVTASCAGGEEGFVYEGRLPFERIITELSELPTVATPILLNVGSPSIAFS
                     CASLPHSGVGLAREEFIINNYIKAHPLALLLHKQVGDANLSSAIRQLIRGFENEEAYF
                     IKKLSYGVAKIAAAFYPKRVIVRFSDFKSNEYRNLLGGIHFEPEEENPMIGWR"
     gene            complement(1860..2861)
                     /locus_tag="VKQ52_06185"
     CDS             complement(1860..2861)
                     /locus_tag="VKQ52_06185"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLZ86804.1"
                     /translation="MKRDMLLVLGACGQPGAELTLALRKIYGPFRVVAADTGPATDSH
                     PNDEPYEQLDVLDKEALASIISRYNITQIYHFATDETATSCQFSTEAWRLNTQGLLNV
                     LALAAEKGINKVFWPGSIAVLGPDAPRQHCPQSAPANPVTPYGISKIAGELWCRYYWM
                     RHGVDVRSLRYPGLISHKKRPGGGEVTDYVTDMFHGALKTQSYTCFLQENTILPMMYM
                     PDAVRATIELMEAPAAAISVRGSYNINAMNFSPRELALAIQRYIPELSITYKPDFRQS
                     IAQGWPVSIDDSVAAADWKWEFTYSIAETVRHMLTHLSTGLDLTPGNIKQLHSQVFF"
     gene            3021..3518
                     /locus_tag="VKQ52_06190"
     CDS             3021..3518
                     /locus_tag="VKQ52_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLZ86805.1"
                     /translation="MLADLDTIDIRILQILQYDARVTTKEMADKLGKSVTAVHERVRR
                     LEDKGYIRRYVALLDRDMIGKTLIGYTTVQLKEHSQAILRGFEKEVIKFPEVMECCQL
                     TGQSDFLLRVVIQDMQAYNDFLLNRLATLPNIGTVHTSFVLSEAKRETAYHLPLPSPR
                     KAKRE"
BASE COUNT          814 a          943 c         1063 g          853 t
ORIGIN      
        1 cgccagccga tcatcggatt ttcttcttcg ggttcgaaat ggatcccccc caggaggttg
       61 cggtattcat tgcttttaaa gtcggagaag cgtacgatca cccttttggg atagaatgcg
      121 gccgctatct tggcgacgcc ataagacaat tttttaataa aataggcctc ttcgttctca
      181 aagccgcgga tcaactgccg gatggcggaa gacaggtttg catcgccaac ctgtttatgc
      241 agcagcaggg ccagcggatg ggcttttata tagttgttga tgatgaactc ttcgcgggca
      301 agacccacac cgctgtgtgg caaagaggcg caggagaagg cgatggacgg tgaaccgacg
      361 ttcagcagga tcggcgttgc cacggtcggc agctcactga gctcggtgat gattcgttcg
      421 aagggaagtc tgccctcgta gacaaaacct tcttcacccc cggcacagga ggcggttacc
      481 ggcatcccat ttgaaagaag ctccgtcgcg gagccgcaac ctactatggc ggggacgccc
      541 atctcccggg cgacgatggc cgcatgacag gtccttccgc ctttattggt aatgatcgcc
      601 gcagccttct tcatgatggg ttcccagtca ggatcggtca tgtcggtgac cagcacgtct
      661 ccttcttgga agtcgctgcc gccgacccgt ttgtccagcg aatacatgat atggaccttg
      721 ccggaggcga tgccgtcacc cacggcgatc ccttttacga cgatcttgtt cgacacagca
      781 tccccgccga ttcggtattc gattagctgg ttcttcggtt tgcgcgaatg aatggtctcg
      841 ggtcttgcct gtacgatgaa caattcgccg gaaagcccgt ccaccgccca ttcgacatcc
      901 acgggaaccc atttaccctt tagatcggaa taatattttt caatcgtcat gaccaagtcg
      961 gcaagctcca ggatatgctt gtcgtccagg cagaatcggt gctgctgcca gggctccacg
     1021 gggatgaccc tcgtccgctc gtcgctgtga tcgccgtata ccatcatctt gtcttttacc
     1081 ccgagccgct tctcgatgat ggagggaaaa ccctgttggc gtgtcggttt gaatacgagg
     1141 aactcatccg gcgacagggc gccctgcact accatttcgc ccaggccaaa ggcgccgttg
     1201 atcattacca cctccctgaa tccggattcg gtatccagcg agaaggccac gccgctgctg
     1261 gcaaggtccg aacgcaccat cttctggaca cagaccgaaa gtccgacagt agatacgtcg
     1321 aagttgaatg tctttcgata gctgatggcg cggtcggtga acagggaggc gaaacagttg
     1381 cggacggcgt cgatcagtgc ggcaggcccg cggacattga gaaaggtctc ctgctgaccc
     1441 gcaaaacttg cgtcgggcag gtcttctgcc gttgccgagg agcggacggc cacgtcggtt
     1501 gacgattgtc cataccgttg cgaaagctgt tcataggcgg cgatgatctg atggcttagc
     1561 tcctggggaa atcgggcatt gctgatggcc tgccggatct tttggccgac gcgacggagg
     1621 gattctacat tgtcgaagtc gatggagttc agctgttgcc ggatggaagg gcccaattgg
     1681 ctctgttcga tgaactgcca ataggcatcg gtgctgacga caaagccgcc ggggatattg
     1741 acgccaagac cggtgagatt tctgagcatt tccccaaggg aagcgcattt gccaccaaca
     1801 agcgccagat cggagagtcc gacggattca agcggaagaa taattctatt ggacatggat
     1861 caaaagaata cttgggaatg aagttgtttt atatttccgg gagtaaggtc cagcccggtg
     1921 gagagatggg tcagcatatg ccggacagtc tcggcgatgc tgtaggtgaa ctcccatttc
     1981 cagtccgctg ccgccaccga gtcatcgatg ctcaccggcc aaccctgggc gatcgactgc
     2041 cggaaatcgg gtttgtaggt aatgctgagt tcgggtatat agcgttggat ggccagggcc
     2101 agctcgcggg gcgagaagtt catggcattt atattgtagg agccgcggac ggagatagct
     2161 gcagccggag cttccatcaa ttcgatggtg gccctgacgg cgtccggcat atacatcatc
     2221 ggcaggatcg tattctcctg caggaaacag gtgtaggatt gggtcttcaa tgcaccatgg
     2281 aacatgtccg tgacataatc cgtcacctct ccgccgccgg gtcttttctt gtgactgata
     2341 agacccgggt atcgaagact gcgaacgtcg acgccgtgcc gcatccaata ataacgacac
     2401 caaagctcgc ccgctatctt gctgatgccg taaggggtaa cggggtttgc cggtgcggat
     2461 tgcgggcaat gttggcgggg ggcatcgggc ccaagaacgg cgatgctgcc cggccagaag
     2521 accttattga tgcctttttc ggctgccagc gccagtacgt tcaggaggcc ctgcgtattg
     2581 aggcgccagg cttctgtgga gaattggcag gacgtggcgg tctcgtctgt tgcaaaatga
     2641 tagatctggg tgatgttgta acgggatatg atgctggcca acgcctcttt atccagcacg
     2701 tcgagctgtt catagggttc atcgttcggg tggctgtcgg tggcggggcc ggtgtcggcg
     2761 gcgacgaccc gaaaggggcc atagatcttc cgcagggcaa gcgtgagctc ggcgccgggc
     2821 tgcccacacg cgcccagcac gagtagcatg tctctcttca tatgcaggca atgtttaagg
     2881 aggtaaaatt ggcgattgcc gttttatttt cctataatta actgaaaagc cgcaaaaaaa
     2941 tctactttta tcccaacatt cggttggtaa atctgaatgg gttggctggg gtagggtcat
     3001 tccgaaaaaa aatctttttt atgcttgccg atctggatac gatcgatata agaattctgc
     3061 aaatattgca atatgatgcc cgggttacca caaaggagat ggccgacaaa ctgggcaaat
     3121 cggtaaccgc cgtacacgaa cgggtgcgac gcctggagga caagggctat atccggcgct
     3181 atgtggcgct actggatagg gacatgatcg ggaagacgct gatcgggtat accaccgtgc
     3241 aactgaagga acattcgcaa gccatattac gtggttttga gaaagaggta ataaagtttc
     3301 ccgaagtgat ggaatgttgt cagttgaccg gacaatcgga ttttctgctc agggtggtca
     3361 tccaggatat gcaggcgtac aatgatttcc ttttgaacag gctggcgacc ttgccgaata
     3421 ttgggacggt gcatacctcg tttgtgctgt ctgaggcgaa gagagagacc gcgtatcatc
     3481 ttccgttgcc ttcgccgcgg aaggcaaagc gggaataaaa gcggaacaag cccatcgctc
     3541 ctcaggcctg cccgcggtga cattgccgtg aggctttcga ctacatgctt tgtcttattt
     3601 tcatcgccca atagtaaacc aattcttgct tttaggaccc caacgagatt tgtcattgcc
     3661 gcactataat gca
//
LOCUS       DATGFG010000329         5270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_146180, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000329 DATGFG010000000
VERSION     DATGFG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146180"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VKQ52_06195"
     CDS             complement(<1..1147)
                     /locus_tag="VKQ52_06195"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLZ86806.1"
                     /translation="MNNGHLPVVVVIGNGMVGHRFCEKLRKAGQFRIVVFGEEPRPAY
                     DRVHLSEYFNGKSASELSLPMTGWFEDEEIVLHLGDPVREIDRQNQLVHTSKGFVQRY
                     DFLVLATGSSPFVPDINGIEKEGVFVYRTIEDLDKIKAYAARSRSGAVMGGGLLGLEA
                     AKALLDLGIPNPHVIEFAPRLMPRQIDSAGSHALESSLRKLGLQLHLNKVTQAIEGNG
                     RVESLQFADESRLAVDMLVISAGIRPRDELARLAGLAVGTRGGIVVSERLQTSDKRIF
                     AIGECALAQGMIYGLVAPGYEMADVVATNLLEGNKTFTGFDMSTKLKLIGVDVASFGD
                     PFAAEPDHGTIVFEDRHKGIYKRINVSRDGKYLIGGVLIGDAAAYNIL"
     gene            1335..2171
                     /gene="cobA"
                     /locus_tag="VKQ52_06200"
     CDS             1335..2171
                     /gene="cobA"
                     /locus_tag="VKQ52_06200"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HLZ86807.1"
                     /translation="MNPVPPASAELILIGAGPGDPELITVKAWRALQKADIILYDHLA
                     NKALLDFARPSCERICVGKRPYGDSISQEEIHELIRQKAGKGGSIIRLKGGDPFIFGR
                     GFEEALFARQLGLRVSYIPGITSMQTAGLCDIPLTHRAVSESIWVVTGTKKDGSLSAD
                     LRLALQSKATVVIYMGMKKLAEIAQLYRQEGVADMPAAIIQHGSWPDQRVAVGTAASL
                     VEMAAANGLSHPAIILIGEVVGLAATSAAAGSAAAVGAEIEQKSHRTIPLYLEESPAA
                     II"
     gene            complement(2218..2871)
                     /gene="fsa"
                     /locus_tag="VKQ52_06205"
     CDS             complement(2218..2871)
                     /gene="fsa"
                     /locus_tag="VKQ52_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HLZ86808.1"
                     /translation="MKFFIDTADLAQIKEANDLGILDGVTTNPSLMAKVGIKGEAAVV
                     NHYKTIAELVDGDVSAEVVSTEFKAIIEEGKKLHAIHPNIVVKVPMIKEGIKALKWFS
                     DNGIKTNCTLCFSAGQAILAAKAGANYISPFIGRIDDSSWDGLELIEQIAEIYAIQGY
                     ETEILAASIRSPLHIVKCAEAGADVITSPLESILGLLKHPLTDIGLAKFLADAKTWA"
     gene            complement(2980..3079)
                     /gene="ffs"
                     /locus_tag="VKQ52_06210"
     ncRNA           complement(2980..3079)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VKQ52_06210"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3124..4398)
                     /locus_tag="VKQ52_06215"
     CDS             complement(3124..4398)
                     /locus_tag="VKQ52_06215"
                     /inference="COORDINATES: protein motif:HMM:NF028426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HLZ86809.1"
                     /translation="MNCKACISLLIFLFTAGGVAAQIPDFIYSPTIGTPQLFTAGNQL
                     GYPIIRLGSSDQLELHFDDIDESVKNYYYTIVLCNEDWTPAQVSEFDFIKGFSQIRIE
                     NYRVSSVALTHYVHYTAMVPDPNCIPIHSGNYMLKVFQDGDTSKLAFTRRFLVTDAKV
                     RIESQLLTPQNFEIARTYQNIQFRLNTSAINPPNPLDQIRVVVLQNDRWDNAIHGVKP
                     RVFLNNDMQYNASDDVNFEGGSEWRWADLQSFRYQSDRVQTAHYGKTSTDIFLIPDGD
                     RSQREFYFYRDYNGGFLIQTTENFNPLYQTDYATVHFVFVPGGNIPLDNKDVYVLARF
                     TGGGINDSSRMVWNAARGGYERNFLLKQGFYSYSYVAVDRSDPSMKASFAQTEGNHVE
                     TENDYMILVYYRALGSRADELVGIATFNSLNK"
     gene            4490..>5270
                     /locus_tag="VKQ52_06220"
     CDS             4490..>5270
                     /locus_tag="VKQ52_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86810.1"
                     /translation="MNVPAFIARRVAFNREKSFSRFIIRLAIAATVISVAAMLLTLAF
                     TNGFQYAISRKVFNLWGHLRVQHFSPNRGLIPEESPMAKNDTVLRVLRSDPDIRTIQA
                     FATKYAVVRSGEGIDYVQVKGVEKAYDFNNLHDFLKAGRWPHFPDSGYSNEIVLSEYT
                     AGQLRIRVGDKVVIYFIRSDGPPRVRPMIVSGLFKTGIEDYDKLLAIADLRLIQRLNN
                     WTPEQIGGYEIFTKDYREAESVNSRIYDQLPKDWGSTTTQES"
BASE COUNT         1191 a         1466 c         1436 g         1177 t
ORIGIN      
        1 gcaggatatt gtacgccgcc gcatcgccga tcagcacccc gccgatgagg tattttccgt
       61 cgcggctgac attgatacgt ttatagatgc ctttatgacg atcctcgaag acgatggtcc
      121 catggtccgg ttcggcggca aaggggtcgc cgaaacttgc cacatccacc ccgatgagct
      181 ttaatttggt gctcatatcg aaaccggtga aggttttatt cccctccagc aggttggtgg
      241 ccaccacatc ggccatctcg taccccgggg ccaccaggcc atagatcatc ccctgtgcca
      301 gggcgcattc gccgatggca aagatgcgtt tgtcggaggt ctgcaatcgc tcactcacca
      361 cgataccgcc cctcgtgcct acggcaagac ccgccagccg ggccagttca tcacgagggc
      421 ggatacccgc cgaaatgacg agcatatcca cggcaagccg gctttcgtcg gcgaattgca
      481 ggctttcgac acggccgttg ccctcgatgg cctgggtcac cttattcaga tgcagttgca
      541 gcccgagttt ccgaagactg gactcgaggg cgtgactgcc cgcgctgtcg atctgccggg
      601 gcatcagccg cggcgcgaat tcgatcacat ggggattggg gatgcccagg tcgagcaggg
      661 ccttggcggc ctccagcccc agcagaccgc cacccatgac ggcgccgctc cgcgaccggg
      721 cggcataggc ctttatcttg tccaggtctt cgatcgtccg gtaaacaaag accccttcct
      781 tctcgatgcc attgatatcg ggaacaaagg gcgaagagcc tgtcgccaac accaggaagt
      841 cataacgctg gacgaagccc ttgctggtat gcaccagctg gttctgccgg tcgatctccc
      901 gcacggggtc tccaaggtga agcacgatct cttcatcctc gaaccagccg gtcatcggga
      961 ggctaagctc cgaggccgat ttgccattaa aatactcgct gagatggacc cggtcgtagg
     1021 caggccgggg ttcttcgcca aaaaccacga tgcggaactg gccggccttg cgcagcttct
     1081 cgcaaaaccg gtgtccgacc attccattgc cgataacgac cactacgggg agatgcccat
     1141 tattcatata cactcaattt tggggataaa cggaaagcag tcgttagata ttagcattta
     1201 ttaaatataa tgccgtacca caagtatagc gattttttta attgaaaata tattttatat
     1261 catataaaaa gtaaaatgat ccgatttttt tggctaattt agtataacga gtaatattta
     1321 aattaattta atatatgaat cccgttccac ccgcttctgc tgaacttatt cttatcggcg
     1381 ccggtcccgg cgatccggag ctgatcaccg taaaggcctg gcgggccctt caaaaagcag
     1441 acatcattct ctatgatcac ctggccaaca aggccctgct cgactttgcc cggcccagtt
     1501 gtgagcgtat ctgcgtcggc aaacgccctt acggggattc catcagccag gaagaaatcc
     1561 acgagctgat ccggcaaaaa gcggggaaag ggggatcgat catccggcta aagggcggtg
     1621 atccctttat ttttggccgg ggtttcgaag aagccctgtt tgcccggcag ttgggtctcc
     1681 gggtctctta tatcccgggc atcaccagta tgcagacggc aggcctatgt gatatcccgc
     1741 tcacccaccg ggcagtgagc gaaagcatct gggtggtcac cggaacgaaa aaggacggtt
     1801 cgctctcggc cgatcttcgg ctggcgctgc aaagcaaggc cacggtggtc atttatatgg
     1861 ggatgaaaaa actggcggaa atagcccagc tctaccggca ggaaggggtg gccgatatgc
     1921 cggcggccat tatccagcat ggctcctggc ccgatcaacg ggtagcggtc gggacggcgg
     1981 cctcgctcgt ggagatggcg gccgccaatg ggctctcgca tccggccatt atccttatcg
     2041 gggaagtggt gggattggct gccaccagcg cggcggcggg ttcggcggcg gcagtagggg
     2101 cagagatcga acaaaaaagc cacaggacga tcccccttta tctggaagag tcgcctgcgg
     2161 cgattatata aggttcgcta atgtctgcgc tatcgcgcaa acctcactcc gttcggttta
     2221 tgcccaggtt ttcgcgtcgg caaggaactt cgccaggccg atatccgtca gcggatgctt
     2281 cagcagaccc aggatggatt ccagcgggga agtgatcaca tcggcgccgg cttccgcaca
     2341 cttgacgata tgcaaggggc tgcggatcga agcggcaagg atctccgtct cataaccctg
     2401 gatggcatag atctcggcga tctgctcgat gagctccagg ccgtcccagc tgctgtcgtc
     2461 gatgcgcccg ataaagggag agatataatt tgcgccggcc ttcgccgcca ggatcgcctg
     2521 acccgcggag aagcaaagcg tacagtttgt cttgatgccg ttgtcggaga accatttcag
     2581 ggcctttatc ccctccttga tcatcggaac ctttaccacg atattcggat ggatggcgtg
     2641 cagtttcttg ccttcttcga taatagcctt gaactccgtg gataccactt cggcgctgac
     2701 atctccgtct accagttctg cgatcgtctt gtaatggttg accaccgcag cctcaccctt
     2761 gatgcctacc ttggccatca gcgaagggtt ggtcgtaaca ccatctaaaa tgcctaagtc
     2821 gtttgcttcc ttgatttgcg ccagatcggc cgtgtcgatg aagaatttca taaaatgtcg
     2881 cttttaaagt gttgctttat tgttgcttat tttggagtat aaagatacgc aatatccttt
     2941 ttcagaggag ggagtcatga tgaccggata caaataaaaa atggcaaagt tacttatatc
     3001 gcaccgctac gaccacctac cgttgctgcc ttccggccct ggcggggttc agcgggagct
     3061 ggtcgtataa gactttgccg gtacaaagat aagcaacaac aaacgaattg atgcaaaaaa
     3121 attttattta ttgagactgt taaaagtggc gatgcccacc agttcgtcgg cgcgggaacc
     3181 cagcgcccgg taatagacaa ggatcatata atcattctcc gtctccacgt gattgccttc
     3241 cgtctgtgcg aatgacgcct tcatcgaagg gtccgaccgg tctactgcga cataggagta
     3301 gctgtagaat ccctgcttca gcaaaaagtt acgttcatac ccccctctcg cggcgttcca
     3361 gaccatccgg ctgctgtcgt tgataccgcc gccggtgaac cgggcaagaa catacacatc
     3421 cttgttatcg agcgggatat tcccacccgg cacaaagaca aaatgcaccg tggcatagtc
     3481 cgtctgatac aggggattga aattctcggt ggtctggatc aggaacccgc cgttgtaatc
     3541 ccggtagaaa tagaactccc gctggctcct gtccccatct ggtatcagaa agatatccgt
     3601 cgatgtcttc ccgtagtggg ccgtctggac acggtcgctc tggtaacgaa agctttgcaa
     3661 atcggcccat cgccattccg aaccgccttc gaaattgaca tcgtcgctgg cattgtattg
     3721 catatcgttg ttgagaaaga cccggggctt caccccatga atggcattat cccagcggtc
     3781 gttctgcagg acgacgaccc tgatctggtc gagggggttc ggcgggttga tggccgaggt
     3841 attcagccgg aactggatgt tctggtacgt tctcgcgatc tcgaagttct gcggcgtcag
     3901 caactggctc tcgatcctga ccttggcgtc cgtgaccagg aagcgccggg tgaaagcaag
     3961 cttcgaggtg tcgccgtcct ggaatacctt taacatataa ttccccgaat ggatcgggat
     4021 gcaattcgga tcgggcacca tcgcggtgta atgcacataa tgcgtgaggg ccaccgacga
     4081 gacgcgataa ttctcgatcc ggatctggga gaaccctttt ataaagtcga attcggagac
     4141 ctgggcgggc gtccagtcct cgttacaaag aacgatcgta taataatagt tcttcacgct
     4201 ctcgtcgatg tcgtcgaagt gaagctccag ctggtcgctg ctgcccagcc tgataatggg
     4261 gtaccctaat tgattgcccg cggtaaagag ctgcggtgtg ccgatggtcg gggaataaat
     4321 aaagtcgggg atctgtgcgg ccacgccacc ggcagtaaaa aggaagatca acagggagat
     4381 acaggctttg caattcatgg gaaaagaatt atcggatata ataataggac aaaaatttca
     4441 ttgatagccc cccacccgtt atttttgacc acctaataac atcatccgtt tgaatgtacc
     4501 tgcctttata gcccgtcgcg tcgccttcaa ccgggaaaag tccttctccc gcttcatcat
     4561 ccgcctggcg atcgcggcga cggtgatcag cgtcgcggcc atgttgctga ccctggcctt
     4621 taccaacggt ttccagtatg ccatcagccg gaaggtgttc aacctctggg ggcatctccg
     4681 ggtgcagcat ttctcaccca accggggcct gatcccggaa gagtcgccca tggcaaagaa
     4741 cgacacggtg ctgcgcgtcc tgcggagtga tcccgatatc aggaccatcc aggcctttgc
     4801 gaccaaatac gccgtcgtcc gcagcggcga aggcatcgac tatgtacagg tgaaaggggt
     4861 ggagaaggcg tatgatttta acaacctcca tgacttcctg aaggccggcc gctggccgca
     4921 tttccccgac agcggctaca gcaacgagat cgttctctcc gaatatacgg cgggtcagct
     4981 gcggatcagg gtcggcgaca aggtggtcat ctattttatc cggagcgacg ggccgccccg
     5041 ggtccggccc atgatcgtgt ccgggctttt caagaccggc atcgaagact atgacaagct
     5101 gctggccatc gccgatctgc gcctgatcca gcggctcaac aactggaccc ccgaacagat
     5161 cggcggctac gagatcttta cgaaggacta ccgggaagcc gaaagcgtca acagccggat
     5221 ctatgaccag ttgccaaagg actggggaag caccaccacg caggagagct
//
LOCUS       DATGFG010000330         4660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_914751, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000330 DATGFG010000000
VERSION     DATGFG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914751"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VKQ52_06225"
     CDS             <1..641
                     /locus_tag="VKQ52_06225"
                     /inference="COORDINATES: protein motif:HMM:NF042854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6805 domain-containing protein"
                     /protein_id="HLZ86811.1"
                     /translation="VTANPDPNQWVHRLSMDTLVFETTAIGRPDDVKLVPFNRTANEY
                     YSVYWDAFTPAAWTIQQQKYEEERKREQELEDKTIDRLRIGEMQPERDHSFAGESLYT
                     GEEHGRKWRSTGDGGWFSFVMKVDPSAGNSILCSYWGDDHRQRIFDIQIDGQTIATQN
                     LTTFKQSKFYEIGYPIPQELVKGKQTVMVKFVAHGAHAGVGPVSGTIRMVRS"
     gene            complement(638..1519)
                     /locus_tag="VKQ52_06230"
     CDS             complement(638..1519)
                     /locus_tag="VKQ52_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ86812.1"
                     /translation="MKVLQFTIPVPHDRTVIVQKDELPHFYPHLHRHQEIQLTWIQQG
                     EGTLVAENNMHTFRPGDIYWLGVNLPHVFKSDPSYFAPASRKKVQTLNIFFNVKGQLD
                     SFFELPEIKNLKNFLQNQQTGFKVPPKYAAEISARMLRMQNSSGVDQLIHFIELLKQL
                     SSFQELEPLSSCSQPASYSEHEGIRIGAIYNFIMQMYDKPITLEDVAKQAHMTPQAFC
                     RYFKKHTLHTFVSFLNEVRINEACKKLTDGNYDSIATVAYNCGFNSITNFNRVFKSIT
                     RKSPTEYVDSYFQQVEA"
     gene            1657..2913
                     /locus_tag="VKQ52_06235"
     CDS             1657..2913
                     /locus_tag="VKQ52_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLZ86813.1"
                     /translation="MRKNVVVVGGGIIGLSSAYYLQKAGHQVTVLDRSDFMDNCSYGN
                     CGYVCPSHFIPLATPGIIRQGLKWMLNSQSPFYVQPRLSRSLFDWGMKFMKSATPEHV
                     ERSAAPLKDIAVLSQYEYENTWLRTPHFDFAYQHKGLLEIFQTEAAAEHAHHTVEKAH
                     ELGLDVDLLDAKELQEKEPHTKVNGLGAIFFKCDAHLYPDKLMRGLIRLLQTSGVELL
                     PHEEVRRFETGNGAVKRLITPLHTFEADAIVIATGSWSREIAGLLDTRLPLVPGRGYS
                     ITLEDSPWRVNYPAILVEGRAAITPMDGNKIRFGGTMEITSTNTPPRYARVQGILNAV
                     KRFFPEFDVPMPPHEKVWYGYRPCSADGLPYIGRIKKYNNVVIATGHSMLGLSLGAGT
                     GKLVSELVDELTPSIDITPFAVERFN"
     gene            2936..>4660
                     /locus_tag="VKQ52_06240"
     CDS             2936..>4660
                     /locus_tag="VKQ52_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HLZ86814.1"
                     /translation="MLRKYLLLQAGLLFALLYTQAQTAVPNPVRPAEGLQSSAWQPDR
                     AGILERYRKARYLDSIATRSIFKATVRPHWSADNTSFWYRNFLQDSVKEYIFVDMVHG
                     TRKKLDAEPADTLTRFTRFKSYTGRRWASFSTDSLSPDKQYVAFVQEGNVFIRPANGT
                     RQDAIAYTTNGDTAKPYGQLAWSPDSKYVVGYHINPVHDSAVYYVLSSVTGTTRGQLR
                     RRPYKQPGDPFTTYEMFLFLLDGHRTQKVNTPPIDFFGAPELHWRHNDSRYFSFERVE
                     RGHQRFRIIEVDAATTDTRTVYDEQTKTFIYESRLFTYYLPETNEVLLTSEKDGWQHL
                     YLIDAVTGAQKQEITRGPWIVRNIDSVDAKKREIWFSACGMNPDEDPYYVHYYRIGFD
                     GRHLLDLTPAKANHIVTFSPDRKYYLDACSEINVPTITGLYRTADGRKVLDVEQADAS
                     LYLSTGLRFPEPFHAKGRDGVTDIWGIIVRPADFDASKRYPVIENIYAGPQDAFVPKD
                     FTGRYGEMQSIAQLGFIVVQIDGMGTANRSKAFHDVCWKNIADAGFPDRIRWIKALAA
                     KYPYVDTTR"
BASE COUNT         1108 a         1453 c         1142 g          957 t
ORIGIN      
        1 tcgttaccgc gaaccccgat ccaaaccaat gggtacatcg gctgagcatg gataccctgg
       61 tcttcgaaac caccgccatc ggccggccgg atgatgtgaa actcgttcct ttcaaccgga
      121 cagcaaacga atattacagc gtctactggg atgcgttcac ccctgccgcc tggaccatcc
      181 aacaacagaa atatgaagag gagagaaagc gggaacagga actggaagac aagaccatcg
      241 accggctgcg catcggcgaa atgcaacccg aacgggacca ctccttcgcc ggcgagagcc
      301 tgtacacggg ggaagaacat ggcaggaaat ggcggtccac cggcgacggc ggctggttct
      361 ccttcgtcat gaaggtagac ccctcggccg gaaacagcat cctctgctct tattggggcg
      421 acgatcaccg ccaacgcatc ttcgacatcc agatcgacgg ccagaccatc gctacccaaa
      481 acctgaccac cttcaaacaa agtaaattct acgagatcgg ttatcccata ccccaggagc
      541 tcgtaaaggg aaaacaaacc gttatggtga aattcgtggc ccacggggcc catgccgggg
      601 tcggaccggt ttcaggaacc atccggatgg tgcggagtta ggcctccacc tgctggaaat
      661 aactgtcgac atattcggtg ggggacttcc gggtgatgct cttgaacacc cggttgaaat
      721 tggtgatgct gttgaacccg cagttgtagg ctaccgttgc gatactatca taattgccgt
      781 ccgtcagctt cttgcaggcc tcgttgatcc gcacctcgtt caggaaggaa acaaacgtgt
      841 gcaacgtatg cttcttgaaa taccggcaga aagcctgggg cgtcatatgc gcctgcttgg
      901 ccacatcctc gagcgtgatc ggcttatcat acatctgcat gataaagtta tagatcgccc
      961 cgatgcgaat gccttcatgc tcgctgtagg aagccggctg cgaacaggaa gagaggggct
     1021 cgagttcctg gaaagaactt agctgtttta acagctcgat aaaatggatc agctgatcga
     1081 cacccgaact gttctgcatc cgcaacatcc tggccgagat ctccgccgca tatttcggcg
     1141 gaaccttgaa gccggtctgc tgattttgca ggaaattctt caggttcttg atctccggca
     1201 gctcgaagaa actatccaac tgccccttca cattgaaaaa gatattgagc gtctggactt
     1261 ttttccggct ggccggcgca aaataggaag gatcgctctt gaacacatgc ggcagattca
     1321 cccccagcca atagatatcc cccggccgga aggtgtgcat attgttctcc gctacaaggg
     1381 tgccctcgcc ttgctggatc caggtgagct ggatctcctg gtgccggtga agatgcgggt
     1441 aaaaatgcgg cagctcgtcc ttttgcacga tgaccgttct gtcatgcggc accggaatgg
     1501 tgaattgcag tactttcatc gcttttttat gaatttacgc catttgcaaa tattatcacc
     1561 gaattatcaa aatcaggcaa aaactggtta aaatacggtt aaaataggat tagaatggca
     1621 aaaccgaccg cccccattta cccttatttt tgctcaatga gaaaaaatgt agtcgtcgtt
     1681 ggcggcggta ttatcggttt gagctctgct tattatctcc aaaaggccgg acatcaggtt
     1741 actgttttag atagatcgga tttcatggac aattgctcat acggcaattg tggttatgta
     1801 tgccccagtc attttatccc tctcgcgaca cccggtatca ttcgccaggg attgaaatgg
     1861 atgctgaatt cccaaagccc tttttatgta caaccccggc taagccgttc actcttcgac
     1921 tggggaatga aattcatgaa aagcgcgaca cccgaacacg tcgaacgctc ggcagcgcct
     1981 ttaaaggaca tcgccgtcct gagtcagtac gaatacgaga atacctggct gcggacacca
     2041 catttcgatt ttgcctatca gcataagggc ctgctcgaga tcttccagac cgaagccgcg
     2101 gcggaacatg cccaccatac cgtggagaaa gcacatgaac tgggactgga cgtcgacctc
     2161 ctcgatgcca aagagctcca ggagaaagag ccgcatacaa aagtgaatgg cctcggcgcc
     2221 atcttcttca aatgcgacgc ccatttgtac cccgataagc tgatgcgcgg gctcatccgc
     2281 ctcctgcaaa cctccggcgt cgaactcctt cctcatgaag aggtccggcg cttcgagacc
     2341 ggcaacggcg ccgtcaaacg cctgatcaca cccctccata cctttgaagc cgatgccata
     2401 gtcatcgcca ccggttcctg gagccgcgag atcgccggcc tcctggatac ccgcctgccc
     2461 ctcgtacccg gccgcggcta ctccatcacc ctcgaagact ccccctggcg cgtcaattac
     2521 ccggccatcc tcgtcgaagg ccgcgccgcc atcaccccca tggatgggaa taaaataaga
     2581 ttcggcggaa cgatggagat cacctccacg aataccccgc cccgttacgc ccgggtacag
     2641 ggcatcctca atgcggtgaa gcgcttcttc cccgaattcg atgtgcccat gccgccccat
     2701 gaaaaagtat ggtacggcta tcgcccctgc tcggccgacg gcctgcccta tatcggccgg
     2761 ataaagaaat acaacaacgt ggtaatagca acgggccact cgatgctggg gcttagcctc
     2821 ggcgcgggta ccgggaaact ggtgagcgaa ctggtggatg aactcacccc aagcatcgac
     2881 ataacgcctt tcgctgtcga acgcttcaac taggtacgac aacaaacaaa catatatgct
     2941 tcgaaaatac ctgcttttgc aggcagggct tctttttgcc ctcctgtaca cccaagcgca
     3001 aaccgccgta ccgaaccctg tccggccggc cgaaggcctc cagtcatcag cctggcaacc
     3061 cgaccgcgcc gggatcctcg aacgctaccg caaggcccgc tacctcgaca gcatcgccac
     3121 ccgctccatc ttcaaggcca ccgtacgacc ccactggtcg gcggacaaca cctccttctg
     3181 gtaccggaat ttcctgcagg acagcgttaa agaatacatc ttcgtggaca tggtacacgg
     3241 cacccgcaaa aaactggacg ccgaacccgc cgataccctg acgcgcttca cccgcttcaa
     3301 atcctacacc ggcagacgct gggcctcctt ctccaccgac tcgctttcgc ccgacaaaca
     3361 atacgtggcc tttgtccagg aaggcaacgt cttcattcgg cccgccaacg gcacccgcca
     3421 ggacgcgata gcctatacca ccaacggcga taccgcaaaa ccctacggcc aactggcctg
     3481 gtcacccgat agcaaatatg tcgtcggcta tcatatcaat cccgtccacg actccgccgt
     3541 atactatgtg ctgtcttccg tcaccgggac aacccgcggc cagctccgcc ggcgtcccta
     3601 taaacaaccc ggcgacccct tcaccaccta cgagatgttc ctctttcttc tcgacggcca
     3661 ccggacacaa aaggtcaata ccccgccgat cgattttttc ggcgcgcccg aattgcactg
     3721 gcggcataac gacagtcgct acttcagctt cgaacgggtg gaacgcggcc accagcgctt
     3781 ccgcatcatc gaggtcgacg ccgccacgac cgatacccgc accgtctatg acgaacaaac
     3841 aaagaccttt atttacgagt cccggctctt tacgtactac cttccggaaa caaatgaggt
     3901 cctgctcacc tccgaaaagg acggctggca acacctctac ctcatcgatg ccgtcaccgg
     3961 cgcccagaaa caggagatca cccgcggacc ctggatcgtt cgcaacatcg acagcgtgga
     4021 tgcaaaaaaa cgggagatct ggttttccgc ctgcggcatg aaccccgacg aagatcctta
     4081 ctatgtgcac tattatcgca tcggcttcga cggccggcat ctcctcgatc tcacccccgc
     4141 aaaggccaac catatcgtca ccttctcgcc cgaccggaaa tactatcttg atgcctgttc
     4201 ggaaataaat gtccccacca tcaccgggct gtaccggact gccgacggca ggaaggtcct
     4261 cgatgtggaa caggccgatg cttcccttta tctgtccact ggtctgcgct ttcccgaacc
     4321 cttccatgca aaaggccgtg atggcgtcac cgatatctgg gggatcatcg tccgcccggc
     4381 tgacttcgat gcgtctaagc ggtatcccgt tatcgaaaac atctacgccg gcccgcagga
     4441 cgccttcgtc ccaaaagact tcaccggccg ctatggcgag atgcagagca tcgcacagct
     4501 gggcttcatc gtcgtccaga tcgatggtat gggtacggcc aaccgctcga aggccttcca
     4561 cgacgtctgc tggaagaata tcgccgatgc cggcttcccc gaccggatac gctggataaa
     4621 ggccctggcg gcaaagtacc cctatgtgga taccacccgg
//
LOCUS       DATGFG010000331         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1268115, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000331 DATGFG010000000
VERSION     DATGFG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268115"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1833)
                     /locus_tag="VKQ52_06245"
     CDS             complement(196..1833)
                     /locus_tag="VKQ52_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86815.1"
                     /translation="MNYIHLKKRFATIGLVALAGSFLLGGCTKRFDEYNTNPYGLSNE
                     DLAADYKLLGQPLEQVQQDIYVAQPAWDTQLQQNLIGDNYSGYMGSPTPFLGNQNNMT
                     YALVDGWNGQPWADAYNDVMSPVQSVLKNAGTTYPVFKAWAKILRVEAMHRLSDIYGP
                     IIYTHYGQVNTDGSITYDSQKDAYYAFFADLDSAIAVFTPLAQNSSSPKTFTNFDLVY
                     GGDYKEWTKFANTLKLRLAIRISQIDPAKAQTEGESALANPIGLLTTPADNFNVNIGT
                     FTHPLNVMNNSWGDCRFGAPLASYLTGYSDPRTPKYATPATDPAVAGKVIGVRNGINI
                     DAKGRYEGYSQPVTFPNYSAASGAANFVQLMTAAEAWFLKSEAALRGWAGAGDAGTDY
                     NSGISASFAQFGLDATSYLNDATSTAAQYIDPKAVTPGENDITTGSPYLSTITIKWDN
                     SATFQQKLERIITQKYIAMFPDGQEAWSEFRRTLYPKLYPNTVNFSNGAIPTAKFIRR
                     INFALSERNTNPNGVADAVKKLGGPDTGGTPMWWDVH"
     gene            complement(1859..>2205)
                     /locus_tag="VKQ52_06250"
     CDS             complement(1859..>2205)
                     /locus_tag="VKQ52_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86816.1"
                     /translation="KATDVTTGNAFSGLLPAQTFFKSVGDRNGLTEFYMYDATAVRLR
                     ELAIGYNVPNKVKWITSLRVSLIGRNLFFIHKDAPFDPETSMSTANTLQGIESFSPPT
                     TRSLGASVKIGF"
BASE COUNT          457 a          583 c          649 g          516 t
ORIGIN      
        1 accgcaaccc cggccccatt gtagaaatac gggttgacca tgacgttcaa ccgctcattg
       61 tcttccttca gcaggttgcg gaagtttatg cccgtacgcg actccggcag ccgcgtaaac
      121 aatctggacc tatcctccat acaggagaat aggcccagca gaaaaataag tatgaaatgt
      181 actctgttca tcgtcttaat gcacatccca ccacatcggc gtaccgcccg tatcggggcc
      241 gcccagcttc ttcaccgcgt ccgcaacccc gttggggttg gtgttcctct cgctgagggc
      301 gaaattgatc cggcggatga acttggcggt tgggatggcc ccgttgctaa agttcactgt
      361 attgggatag agtttgggat acagggtccg cctgaactcc gaccaggctt cctgtccgtc
      421 ggggaacatc gcgatatatt tctgggtaat gatccgctcc aatttttgct ggaaggtagc
      481 ggaattgtcc cattttatcg taatggtgct cagataaggc gaaccggtgg tgatgtcatt
      541 ctcgcccggg gtgacggcct tcggatcgat gtattgcgcc gccgtgcttg tggcgtcgtt
      601 gagataactg gtggcatcga ggccaaattg ggcgaacgag gcgcttatcc cgctgttata
      661 gtccgtccct gcatcgcctg caccggccca gccgcgaagc gccgcttccg atttcaggaa
      721 ccaggcttcg gcggccgtca tcagttgcac aaaattggcg gccccgctgg cggcactgta
      781 attggggaag gtgaccggct gggaatagcc ttcataccgg cccttggcat cgatgttgat
      841 cccgttgcgt acgccgatga ccttgccggc gacggcgggg tcggtggccg gagtggcata
      901 cttgggcgtc ctcggatcac tgtaaccggt gaggtaggag gccaatgggg cgccgaaacg
      961 gcaatccccc caggaattgt tcatgacatt gaggggatgt gtgaacgtcc cgatattgac
     1021 gttgaaattg tcggccggtg tcgtcagcag gccgatggga ttggcaagcg ccgactcgcc
     1081 ttccgtctgg gcctttgccg ggtcgatttg cgaaatgcgg atagccagcc gcagcttcag
     1141 cgtattggcg aatttggtcc actccttgta atcaccgcca tagaccaggt cgaaattcgt
     1201 aaaggtcttt ggcgaactgc tgttctgcgc caggggcgta aacacggcga tcgcggaatc
     1261 gagatcggcg aaaaaggcat aataagcatc cttctgggag tcataggtga tgctgccatc
     1321 ggtattcacc tggccataat gcgtatagat gatcgggcca tagatatcac tcaggcgatg
     1381 catggcctcc acgcggagga tcttcgccca ggccttgaac acgggataag tggtcccggc
     1441 gttcttgagc acggactgta cgggcgacat cacatcattg taggcatcgg cccagggctg
     1501 gccattccat ccgtccacca gggcgtaggt catgttgttc tggttgccga gaaagggggt
     1561 cgggcttccc atatagccgg agtagttgtc gccgatgagg ttttgctgca gctgcgtatc
     1621 ccaggcgggc tgcgccacat agatatcctg ttgcacctgt tcgagcggct ggcccagcaa
     1681 tttgtagtcc gcggccaggt cctcgttgga caggccatag ggattcgtgt tgtactcgtc
     1741 aaatcttttc gtgcaaccgc ccagtaaaaa actgcctgcc agggccacga ggccgatcgt
     1801 cgcaaagcgc tttttaagat gtatatagtt cataatgctt gtgattaaag tttaaatgtt
     1861 agaacccgat ctttacactt gccccgaggc tccgcgttgt gggcgggctg aagctttcga
     1921 tcccctgcag ggtattggcg gtggacatcg acgtttccgg atcgaaggga gcatccttat
     1981 ggatgaagaa gagattcctg ccgataaggg agacccgcag cgaagtgatc catttgacct
     2041 tgttgggaac attataaccg atagccagct ctctcaaccg caccgcagtc gcatcataca
     2101 tatagaattc ggtaagcccg tttctgtcgc cgacactctt gaagaaggtc tgggcgggaa
     2161 ggaggccgga aaaagcattt ccggtggtga catcggtggc tttca
//
LOCUS       DATGFG010000332         4958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_336190, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000332 DATGFG010000000
VERSION     DATGFG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336190"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VKQ52_06255"
     CDS             <1..1380
                     /locus_tag="VKQ52_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ86817.1"
                     /translation="LLNTFALALAAISAILLAPAFDNLIGSDAGSFHLPAIYWLGFAG
                     IFLLGSLLSGLYPAFVLSGYHPITVLKGLFKNTGRGQILRKGLIIGQFAVSVILITGT
                     ILVYQQVQYMRRQQLGANINQTLVVEGAASPLDSVYANVFQPFKTTLLRQTGIKSVAA
                     SSIVMGQEIYWTRGVRRLGDDANAAVTLYLMGIDYDFLPAYDLRLVAGRNFSQQFPTD
                     KQAALINETALHQLGFKTAAEALDAKIKPGHDTLHIIGVLADYHHQGLQKAINPMLVL
                     NIPNIRSYYSIKFTTSQPHETIAVIKKTWDRYFPQDPFNYFFLDESFNQQYKADTRFG
                     ETFGLFATLAIIIACFGLLGLSAYNVLQRTREIGIRKVLGATEQHLVFLLSKEFLWLV
                     LLSFIIAIPLCWWVMYTWLQDYAYRISIQWWVFALGGFLALLIALTTVGIQAARAALA
                     NPVKSLRSE"
     gene            1423..3837
                     /locus_tag="VKQ52_06260"
     CDS             1423..3837
                     /locus_tag="VKQ52_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86818.1"
                     /translation="MLNNYFKIAWRNLIRQKAYTTINIIGLTLGISAAILIFTLVSYQ
                     LSFDGFHPNKDRIYRVITDLNGETLSHIPCAPQPLGKAFRTDYAYAEKTARIISYRNT
                     LVSLPAPAAGPNSTLSAEQKKFEEENGAAFTEPAFFDIFDFPLLRGDKRTALQNPNSA
                     ILTESLAKKYFGDTDPMGRTIRINNSIDFTITGILKDLPPNTDRKQELYLSYDNLKDW
                     NRSYASDSSWGNLYTGSNCFVLLRPGVKPAAVDKALLAMAKKYLDAEGAAYTNYHLQP
                     LSDIHTDTSVDGVFDKKYSWTLLVIAVFLLATACMNFINMATAQALNRGKEVGIRKVL
                     GSLRNQLFWQFIAETALITLTAGLLAWGIAIAALPALNQLTFAKMSLQLFSTWRTMAF
                     FFGIIILVVFAAGSYPGLVLARFEPILALKSKLSQAHIGGFSLRRLLVIVQFSISQVL
                     IISMIILAWQMHYSMSLDMGFDKDAIVTVPLPVTDLTKMHTLRTRLASIPGVEKVSLC
                     MQPPASRRNQNTDIVFDTRQKSERWDINVKAADDQYLSTFGLKLAVGRNLYPSDTTRE
                     YLVNETAVKKLGLASPLDALGKIISVNDEKAPIVGVVKDFHTYSLKADIAPVCIATTA
                     ARYQNCCLKVDPRHLRQVLASTESTWNNTYPEYLYSYAFLDTRIADFYFLDSILLKLV
                     ELFAGIAILISCLGLYGLVSFMAVRRTKEIGVRKVLGASVTQISWLFGKEFVRLIAIA
                     FVIAAPLAWMATHSYLQDFKYRITVSPLLFLAGLATTIAIAALAVGYRSLIAANANPV
                     NSLRSE"
     gene            3975..>4958
                     /locus_tag="VKQ52_06265"
     CDS             3975..>4958
                     /locus_tag="VKQ52_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86819.1"
                     /translation="MLSNHLKTALRNVWKQRATNAINITGLAAGMTAAILIFLWVGNE
                     LTYDSYHPGADRIYRITSHITSANWIWESAPYPIIAATRASVPEAEAVTAIEAAYGTT
                     IRQGDEFFTEKQSAYVDSAWFSVFHYDFVAGSPRDFFSSPFSLILTRSKAKQYFGDKD
                     PIGRTLRLDTVDYKVAGIIKDIPANSSFTFDFLMPIEALLSNPDRRTNTDTWGNFNLQ
                     VYMRLRPGALPEKVGQTLTQLLHTNAPDKGDARNFLSLIGLKDLHFETDLTFSGGKIN
                     HTNRGNVTIFSILSIFLLVIACINYVNLTTARASLRAKEVGIRKIIGAEKKS"
BASE COUNT         1118 a         1936 c         1004 g          900 t
ORIGIN      
        1 ctgctgaaca ccttcgccct ggcactggca gccatctccg ccatcctcct ggcccccgcc
       61 ttcgacaacc tcatcggcag cgacgcaggc tccttccacc tcccggcaat atactggctg
      121 ggcttcgccg gcatcttcct cctcggctcc ctcctgtccg gactctaccc cgccttcgtc
      181 ctgtccggct accaccccat caccgtcctg aaaggcctct tcaaaaatac cggccgcggc
      241 cagatcctcc gcaaaggcct catcatcggg cagttcgccg tatccgtcat cctcatcacg
      301 ggcaccatcc tcgtctatca acaggtacaa tacatgcgcc gccagcagct cggcgccaac
      361 atcaaccaga ccctcgtcgt cgaaggcgcc gcctccccac tggactcggt ctacgcaaac
      421 gtcttccagc ccttcaagac aaccctgctc cggcaaaccg gcataaaaag cgtcgccgcc
      481 tcctccatcg tcatgggcca ggagatctac tggacaaggg gcgtccggcg cctcggcgat
      541 gacgcaaacg ccgccgtcac cctctacctg atgggcatcg actacgactt cctccccgcc
      601 tacgatctcc gcctcgtcgc cggcaggaac ttctcccaac aattcccaac cgacaaacag
      661 gccgccctca taaacgaaac agccctccac caactgggct tcaaaaccgc cgcggaggcc
      721 ctcgatgcaa agatcaaacc cggccacgat accctccata tcatcggggt gctggcagat
      781 taccaccacc agggcctgca aaaagccatc aaccccatgc tcgtcctgaa tatccccaac
      841 atccgcagct actactcgat aaaattcacc accagccaac cccacgaaac catcgccgtc
      901 ataaaaaaga cctgggaccg ctacttcccc caggacccct tcaactactt cttcctcgac
      961 gaatccttca accagcaata caaagcggat acccgtttcg gtgagacctt cggcctcttc
     1021 gccaccctcg ccatcatcat cgcctgcttc ggcctcctcg gtctctccgc ctacaacgtc
     1081 ctccaacgca cccgcgagat cggcatccgc aaggtcctcg gcgccaccga acaacacctc
     1141 gtattcctgc tgtcaaagga attcctctgg ctcgtgctcc tctccttcat catcgccatc
     1201 ccgctctgct ggtgggtgat gtacacctgg ctccaggact atgcttaccg catctccatc
     1261 caatggtggg tcttcgccct cggcggcttc ctcgccctgc tcatcgccct caccaccgta
     1321 ggcatccagg cggcgagagc agctcttgcc aacccagtaa aaagccttcg aagtgaataa
     1381 attcctcacc gcctggcatc cgccaaccca aacaccaaaa ccatgctcaa caattatttc
     1441 aaaatagcct ggcgcaacct catccgccaa aaagcctata ccacgatcaa tatcatcggc
     1501 cttaccctcg ggatttccgc cgccatcctc atctttaccc tcgtatccta ccagctcagc
     1561 ttcgatggct tccacccaaa caaggaccgc atctatcgcg tcatcaccga ccttaacggc
     1621 gagaccctta gccatatccc ctgcgcgccc caaccgctcg ggaaagcctt ccggacagac
     1681 tacgcctacg ccgaaaaaac cgcccggatc atctcctatc gcaataccct cgtctccctg
     1741 cccgcacctg cggccggtcc caactccacc ctgtccgccg aacaaaaaaa attcgaagag
     1801 gagaacggtg ccgcctttac cgaacccgcc ttcttcgaca tcttcgactt ccccctcctc
     1861 cgcggcgaca aacggacggc cctccaaaac ccaaacagcg ccatcctcac cgaatccctt
     1921 gcaaaaaaat atttcggcga caccgacccc atgggccgga ccatccgcat caataacagt
     1981 atcgacttca ccatcaccgg catcctgaaa gacctccccc cgaacaccga ccgcaaacag
     2041 gaactctacc tctcctacga taacctgaag gactggaacc gctcctacgc cagcgacagc
     2101 agctggggca acctctacac cggcagcaac tgcttcgtcc tgctccgccc cggcgtcaag
     2161 cccgccgccg tcgacaaggc cctcctcgcc atggccaaaa aatatctcga cgcggaagga
     2221 gccgcataca ccaattacca tctccaaccc ctctccgaca tccacaccga taccagcgtc
     2281 gacggggtct tcgacaaaaa atacagctgg accctcctgg tcatcgccgt cttcctcctc
     2341 gccaccgcct gcatgaactt catcaacatg gccaccgcac aagccctcaa ccgcggtaaa
     2401 gaggtaggca tccgcaaagt attgggcagc ctccgcaacc agctcttctg gcagttcatc
     2461 gccgagacgg ccctgatcac cctcacggcc ggcctcctcg cctggggcat cgccatcgcc
     2521 gcgctccccg cccttaacca gctcaccttc gcgaaaatga gcctccagct cttctccacc
     2581 tggcgcacaa tggctttctt cttcggcatc atcatactcg tggtcttcgc cgccggctcc
     2641 taccccggcc tcgtcctggc ccggttcgag ccgatcctcg cgctaaaaag caagctctcc
     2701 caggcccata tcggcggatt ctccctccgc cgcctgctcg tcatcgtcca gttcagcatc
     2761 tcccaggtac tgatcatcag catgatcatc ctcgcctggc aaatgcacta ttccatgagc
     2821 ctcgacatgg gcttcgacaa ggacgcgatc gtcaccgtcc ccctacccgt caccgatctc
     2881 acaaaaatgc ataccctccg cacccgcctc gcctccatcc ccggcgtgga aaaggtcagc
     2941 ctctgtatgc aacctccggc ctcccgccgc aaccagaaca ccgacatcgt atttgacacc
     3001 cggcaaaagt ccgagcgctg ggacatcaac gtaaaagccg ccgacgatca atacctctcc
     3061 accttcggtc tcaagctggc agtcggccgc aacctctacc cctccgatac cacccgcgaa
     3121 tacctggtca acgaaaccgc cgtaaaaaag ctcggcctgg cctcccccct cgacgcattg
     3181 ggaaagatca tctccgtcaa cgacgaaaaa gcccccatcg tcggcgtggt aaaagacttt
     3241 catacgtatt ccctgaaagc cgatatcgcc cccgtctgca tcgccaccac agccgcccgc
     3301 taccagaact gttgcctcaa ggtcgatccc cgccatctcc gccaggtgct ggcatccacc
     3361 gaatccacct ggaacaacac ctatcccgag tacctctatt cctatgcgtt cctcgatacc
     3421 cgcatcgccg acttctattt cctcgacagc atcctgctaa agctggtaga gctcttcgcc
     3481 ggcatcgcca tcctgatcag ctgcctcgga ctctatggcc tcgtatcctt catggccgtc
     3541 cgccgcacaa aggagatcgg cgtccggaag gtcctcggcg ccagcgtcac ccagataagc
     3601 tggctcttcg gcaaagaatt cgtccgcctc atcgcgatcg ccttcgtcat cgccgccccg
     3661 ctcgcctgga tggctaccca tagctacctg caggatttta aatacaggat caccgtcagc
     3721 cccctgctgt tcctcgccgg ccttgccacc accatcgcca tcgccgccct ggccgtcggc
     3781 taccggtcac tcatcgccgc caacgccaac ccggtaaatt ccctgagaag cgaataaaat
     3841 gcaacaaaac caccatggca ggcggccctg atcccaccca attgcaacaa atcggccatg
     3901 gcaggcggcc tcaacacctc cccatgcaac aaaacgacca tcccatgcga ccatagcgta
     3961 cacaaccaac cgacatgctc tccaaccatc tcaaaaccgc cctccgcaac gtgtggaaac
     4021 agcgcgcgac caacgccatc aacatcaccg gcctcgccgc cggcatgacc gccgccatcc
     4081 tcatcttcct ctgggtcggc aacgaactca cctacgacag ctaccacccc ggcgccgacc
     4141 gcatctatcg gatcacttcc catatcacca gtgccaactg gatctgggaa agtgccccct
     4201 atcccatcat tgccgccacc cgcgcctcgg tccccgaggc agaagccgtg acggcgatcg
     4261 aggccgccta cggcaccacc atccgccagg gtgatgaatt cttcaccgaa aaacaaagcg
     4321 cctatgtcga cagcgcctgg ttcagcgtct ttcactatga cttcgtcgcc ggcagccccc
     4381 gggatttctt cagctcgccc ttcagcctca tcctcacccg ctcgaaagca aaacaatact
     4441 tcggtgacaa agaccccatc ggccgcaccc tccgcctcga caccgtcgat tacaaagtgg
     4501 ccggcatcat aaaagacatc cccgccaaca gcagcttcac cttcgatttt ctaatgccca
     4561 tcgaagcact cctgtccaac cccgatcgcc gtaccaacac agatacctgg ggcaacttca
     4621 acctccaggt ctatatgcga ctccgccccg gcgccctgcc cgaaaaggtc ggccagaccc
     4681 tcacccaact cctgcacacc aacgcccccg acaaaggaga tgcccgaaac ttcctctccc
     4741 tcatcgggct gaaggatctc catttcgaaa cggacctcac cttcagcggc ggcaagatca
     4801 accacaccaa ccgcggcaac gtcaccatct tcagcatcct gagcatcttc ctgctggtca
     4861 tcgcctgcat caactatgtc aacctcacta cggcaagggc aagcctccgc gcaaaagagg
     4921 tcggcatccg caagatcatc ggcgcggaaa agaaaagc
//
LOCUS       DATGFG010000333         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_895993, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000333 DATGFG010000000
VERSION     DATGFG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895993"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1142)
                     /gene="dusB"
                     /locus_tag="VKQ52_06270"
     CDS             complement(75..1142)
                     /gene="dusB"
                     /locus_tag="VKQ52_06270"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HLZ86820.1"
                     /translation="MYFCTPIVINVGKIQISAFPLLLAPMEDVSDPPFRYVCKHNGAD
                     LMYSEFISSEGLIRDAIKSRQKLDFFEYERPFGIQIFGGDEEAMAMSARIVDTVQPDL
                     VDINFGCPVKKVVCKGAGAAVLKDVAAMTRLTAAVVKSTSLPVTVKTRLGWDDQSKNI
                     EEVAERLQDVGIQALTIHGRTRAQLYKGEADWTLIGKVKSNPRIQIPIFGNGDIDSPE
                     KARLYRDRYGVDGLMIGRAAIGYPWIFNEIKHFLQTGEHLPPPGIEQRIGAIRDHLLR
                     SVEWKGPVVGILEMRRHYTNYLKGFPYIKEFRNQLVQKKTVEEIDEVLQQVQQRYTGF
                     IPERSFAGFPQDQLDAVPCAY"
     gene            1259..>2084
                     /locus_tag="VKQ52_06275"
     CDS             1259..>2084
                     /locus_tag="VKQ52_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HLZ86821.1"
                     /translation="MSNNIKIPSPWAQLGLFLRLIGFALIGLVALALLFGQLPQPLSS
                     NNLKLAQVANSILLFGVPAYFYARLTFPDRPFFYLGFRPAVKGNFYLLAILLLLFSFP
                     LEGWLGILNKHIPLPHWMVQTEESIDHQLTTLLQVKNPFDVVINLLVIAVIPGIFEEM
                     CFRGVVQRIMIRLTKSPWAGIVVTGFIFSFMHFEFQGFLPRMFLGVLLGAAYHYSGSL
                     WTCVLAHCFFNGIQVLAFTWYPTVMNNDNPSVPAYSVLISSIIVVILLVVMRRQSNQ"
BASE COUNT          378 a          572 c          617 g          517 t
ORIGIN      
        1 aggcgcgagg ggcagcagca ggctggcggg ctgaaggcca atcgttggcg gagcgggcca
       61 gcgggttatt cctgctagta cgcgcagggg accgcgtcca actgatcctg tgggaagccg
      121 gcgaagctgc gctcggggat gaagccggta tagcgctgtt gtacctgctg cagcacttcg
      181 tcgatctctt cgacggtctt tttctgcacc agctggttgc ggaattcctt tatataaggg
      241 aaacccttga ggtagttggt ataatgccgc cgcatttcca ggataccgac cacgggaccc
      301 ttccattcca ccgagcgcag gagatgatcg cggatggcgc cgatgcgttg ttcgatgcct
      361 ggcggcggaa ggtgttcgcc ggtttggagg aaatgtttta tctcgttgaa gatccaggga
      421 taaccaatgg ccgcgcggcc gatcatcagt ccgtcgacgc cgtagcgatc gcgatagagc
      481 cgggcttttt cgggggagtc gatatcgccg ttgccgaaga tggggatctg gatacggggg
      541 ttcgatttca cctttccgat gagggtccag tcggcctcgc ccttgtagag ctgggcgcgg
      601 gtgcggccgt ggatggtcag ggcctggata ccgacgtcct gcaaccgttc ggcgacctct
      661 tcgatgttct ttgactggtc gtcccagccg aggcgggtct ttaccgtcac ggggagggag
      721 gtgcttttaa ccacggccgc cgtaagccgg gtcatggcgg ccacgtcttt cagcacggcg
      781 gcgccggcgc ccttacagac gacctttttc accgggcacc cgaaattgat atcgacgaga
      841 tcgggttgca cggtatcgac gatccgcgcg ctcatggcca tggcttcttc gtcgccgcca
      901 aagatctgga tgccaaaggg gcgttcatat tcgaagaagt ccagcttttg ccggctcttt
      961 atggcatccc ggatcagtcc ttcgctggaa atgaattcgc tgtacatcag gtcggctccg
     1021 ttgtgcttgc acacatagcg aaaggggggg tcgctcacgt cttccatcgg ggccaggagg
     1081 agtgggaaag ctgaaatttg tatcttgccg acgtttatca cgattggggt gcaaaaatac
     1141 acacttatcg tggaattgcc aatgcggacc cgggtccgca ggccgaagac ccatcgttag
     1201 accgtgcacc ggggatgggt tgttttttcg gagaaaacca tccccggtgt caccgaatat
     1261 gtcaaataat atcaaaatcc cctctccctg ggcgcaactg gggctgtttt tacgactgat
     1321 cggctttgct ttgatcgggt tggtcgccct ggccctcctt tttgggcagc tgccacagcc
     1381 cctgtccagt aacaacctca agttggcgca ggtggcgaac tcgatcctgt tattcggcgt
     1441 gccggcctat ttttatgccc ggttaacttt tcccgatcgc ccgttctttt acctgggttt
     1501 ccggcccgcg gtgaagggga atttctacct cctcgccatc ctgctcctgc tgttctcctt
     1561 tccgctggag ggttggctgg gcatcctgaa taaacatatc cccctgccgc actggatggt
     1621 ccagacggaa gagtcgatcg atcaccaact gaccaccctc ctgcaggtga agaacccctt
     1681 tgacgtggtg atcaacctgc tggtgatcgc cgtgatcccg ggcatcttcg aggaaatgtg
     1741 tttccggggt gtcgtgcaac ggatcatgat ccggctcacg aagagtccct gggcgggcat
     1801 cgtcgtcacc ggttttatct tttcctttat gcatttcgag ttccagggtt ttttgccgcg
     1861 gatgttcctt ggcgttctgc tcggcgcggc ctaccattat agcggcagcc tctggacctg
     1921 tgtcctcgcg cactgttttt tcaacgggat acaggtgctg gccttcacct ggtatcccac
     1981 ggtgatgaat aacgataatc cttccgtccc ggcctattcc gtcttgataa gttcaataat
     2041 agtcgtaata ttactggtgg tgatgcggag gcaatcgaat caat
//
LOCUS       DATGFG010000334         5824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_319767, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000334 DATGFG010000000
VERSION     DATGFG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319767"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VKQ52_06280"
     CDS             <1..885
                     /locus_tag="VKQ52_06280"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HLZ86822.1"
                     /translation="LLMGLLCAREYFSGNTPLEVYFRNRVTAMWSVADWNWHTRGDDK
                     LLYWHWSPANDFDMNFPVYGWDEALITYILSAASPTHPISKQLYENSWVRSSSWRNGK
                     SYYGITLPLGNFDYGGPLFFEQYTFMGIDPSGLKDDHGIDYAEQTRNHTLINRAYCID
                     NPKKFKGYSAECWGLTAGDSYKGYVAHCPQDDRGVIQPTAALSSFPYTPEYSMQALKH
                     LYYDLGDKIWGPYGFADGFSESHNWYAKTHLAIDEGPIVVMIENYRSGLIWNLFMQIP
                     DVQRGLHNLGFTSPKIKN"
     gene            890..1717
                     /locus_tag="VKQ52_06285"
     CDS             890..1717
                     /locus_tag="VKQ52_06285"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLZ86823.1"
                     /translation="MNDFQLTPGQIAAYERDGYIIVKNFLSAAEIEKLYGIATGDALL
                     RKHAFDLNDQSGKKTKLTLWYNPGDDAYGLLTRSKKMVVSAGQLLDGDTPVCHFHSKL
                     MQKEPKVGGAWEWHQDYGYWYKNEFLFPDEMISVMVAITKATRQNGCLQVIKGTHKMG
                     RIEHGFSGEQVGASQHYVDLALRTMPLVYVELEPGDALFFHSNLLHRSEANLSDTARW
                     SLISCYNRQSNIGYNEPNGSHTTPVHVVPDEALMEWDTTGVQESAGFLEKDKDEALK"
     gene            1723..3480
                     /locus_tag="VKQ52_06290"
     CDS             1723..3480
                     /locus_tag="VKQ52_06290"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HLZ86824.1"
                     /translation="MKHRVLFLLVILLTCLPGSSQQKTYCNPVNIDYGYTPIPDFSEW
                     GRHRATADPVIVTYKGDYYLFSTNQWGYWWSHDMLDWHFNSRKFLRPWNKAYDELCAP
                     AVGILGDTMIVFGSTYTRNFTIWMSTDPKGNQWKPLVDSFGIGGWDPAFFTDDDGRLY
                     MYNGSSNSYPLYSIELDRATLRPIGTRQETYRLEDWRYGWQRFGEYYDNTFLDPFIEG
                     SWMTKHNGKYYLQYGAPGTEFSGYADGVVVGDRPLGNQWTPQSDPLSFKPGGFARGAG
                     HGATFQDLLGNYWHVSTITIAVKNNFERRIGIWPAGFDKDDVMYCNTSYGDYPHFLPA
                     ERTAEYNAPTGNGGFTGWMLLNYNKPVQVSSTTGAFTANFAVDENIKTYWSAATGDPG
                     EWIRTDLGRLSTVNAIQVNYADQDAVFLGKQTDIYHQYVLYASTDGKKWTVLVDKSRN
                     GTDVPHDYIELEHPVQTRYIKMENRHMPSGKFAISGLRVFGNGGGERPDSVKQFIVLR
                     TEKDKRSAWIKWRPIDNAYAYNIYFGTDPGKLYNSIMVYNANEYWFKVMDSKKPYYFS
                     IEALNENGVSPRTTIVKIN"
     gene            3495..5804
                     /gene="bglX"
                     /locus_tag="VKQ52_06295"
     CDS             3495..5804
                     /gene="bglX"
                     /locus_tag="VKQ52_06295"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HLZ86825.1"
                     /translation="MKAAKTIIAFLLFAAITARGQAPTARAQAPTAGTDAKMNTFVST
                     LMGRMTLDEKIGQLNLPGAGDITTGQAGNSNIAKKIEQGLVGGLFNIKSVEKIKEVQR
                     IAVEKSRLKIPLIFGMDVIHGYETVFPIPLGLSCSWDMAEIEKSARVAATEASADGIC
                     WTFSPMVDIARDPRWGRIAEGNGEDPYLGSQIAKAMVKGYQGDLSGKSDIMACVKHFA
                     LYGAAEAGRDYNTTDMSEIRMFNEYFPPYQAAVDAGAGSVMASFNDVNGTPATANKWL
                     MTDVLRHRWGFKGFVVTDYTGLNELIPHGLGDLQTVSALALKAGIDMDMVGEGFLTTL
                     KVSLQEGKITLAEIDKACRRVLEAKYKLGLFGDPYKYCDEKRAATEIFTDANRSTARQ
                     IAADCFVLLKNQGNLLPLKKGATIALVGPLGDNKENMPGTWSVAADFSKSISLLQGMK
                     DVAGDGTKILYSMGSNLDADSLFEVRAGMFGKALHHDSRPAADIIHDALTVAGQADVI
                     VAALGESAEMSGESSSRSHIDLPQAQQDLLKALLATGKPVVLVLFTGRPLAIKWENDA
                     TPAILNVWFGGSEAGHAIADVLFGTVNPSGKLTTTWPQNVGQVPLYYNHMNTGRPLEG
                     HWFQKFRSNYLDVSNDPVFPFGYGLSYTHFSYSEVTVSSKNLKGDQTLSATVTVTNDG
                     QVQGKEVVQLYIRDLVGSITRPVSELKGFQKIDLKPGESRQVTFSITPRDLKFYNADL
                     KYDWEPGDFVIMIGGNSRDVRSAKINWTK"
BASE COUNT         1437 a         1809 c         1488 g         1090 t
ORIGIN      
        1 ctgctgatgg gactgctttg cgcaagggaa tatttcagcg ggaatacccc gctggaagtc
       61 tatttccgca accgcgtcac ggcaatgtgg tcggttgccg actggaactg gcataccagg
      121 ggcgacgata aattgctgta ctggcattgg agccccgcca atgacttcga tatgaacttc
      181 cccgtctatg gctgggacga agccctcatc acctatatcc tgtccgccgc ttcccccacg
      241 cacccgatct ccaaacagct gtacgagaat tcctgggtcc ggagcagctc ctggcgcaac
      301 ggcaaaagct actacgggat taccctgccg ctcggcaact tcgactatgg cggtccgctg
      361 ttcttcgaac aatatacttt tatgggcatc gatcccagcg gcctgaagga cgaccacggt
      421 atcgactacg ccgaacaaac gcgtaaccat accctgatca accgggctta ttgtatcgac
      481 aacccaaaaa aattcaaagg ctattcggcc gaatgctggg gactcaccgc cggggatagc
      541 tataagggct atgtcgccca ctgcccccag gacgaccggg gtgtcatcca gcctaccgcg
      601 gcgctctcct ccttccccta cacccccgaa tacagcatgc aggccctgaa gcatctttac
      661 tacgacctgg gcgataagat ctggggcccc tatggttttg ccgatggctt tagcgaaagc
      721 cacaactggt acgcaaagac ccatctggcg atcgacgaag gccccatcgt cgtcatgata
      781 gagaattacc ggagcggact catctggaat ttattcatgc aaatcccgga tgtccagcgc
      841 ggattgcata acttaggatt cacctctccc aaaataaaaa actagccata tgaacgactt
      901 tcaactgaca cccggccaga tagccgccta tgaacgggat ggctatatca tcgtaaagaa
      961 cttcctttcc gccgccgaga tcgagaaact gtatggcatt gccaccgggg acgccttact
     1021 gcgaaaacat gccttcgacc tcaacgacca gtccggcaaa aagacaaagc tcaccctctg
     1081 gtacaacccg ggcgacgacg cctatggcct gctcacccgc agtaaaaaga tggtggtatc
     1141 ggccggtcaa ctactcgacg gcgacacccc cgtatgccat tttcacagca agctgatgca
     1201 aaaagaacca aaggtcggag gcgcctggga atggcaccag gactatggat actggtataa
     1261 gaacgaattc ctgttccccg acgagatgat ctccgtcatg gtggccatca caaaagcgac
     1321 ccggcagaac ggctgcctgc aggtgataaa aggcacacat aagatggggc gcatcgaaca
     1381 cggattctca ggggaacagg taggcgcctc ccagcactat gtcgacctgg ccctccggac
     1441 catgccgctc gtatacgtgg agctggagcc gggtgacgcc ctcttcttcc atagcaacct
     1501 gcttcaccgg agcgaagcca acctgtccga taccgcgaga tggtccctga tctcctgcta
     1561 caaccggcaa agcaatatcg ggtataatga accaaacggt tcccatacca cccccgttca
     1621 tgtcgtaccc gacgaggccc tcatggagtg ggacaccaca ggcgtgcagg agtcggccgg
     1681 cttcctggaa aaagataagg acgaagccct taaataaaga ctatgaagca tcgcgttctc
     1741 ttcctgctgg tcatcctcct aacctgcctg ccgggatctt ctcaacaaaa gacgtattgc
     1801 aacccggtga atatcgacta tggctataca cccatccccg atttcagcga gtggggccgc
     1861 caccgggcga ctgccgaccc ggtgatcgtc acctataaag gggactatta tcttttcagc
     1921 accaaccaat ggggctactg gtggagccac gacatgctcg actggcattt caattcccgg
     1981 aaattcctgc gcccctggaa caaggcctat gacgaactct gcgcccccgc cgtcggcatc
     2041 ctcggcgaca cgatgatcgt ctttgggtct acctataccc gcaatttcac catctggatg
     2101 agcaccgatc caaaaggcaa ccagtggaaa ccgctggtcg actctttcgg gatcgggggc
     2161 tgggacccgg ccttctttac cgatgacgac ggccgcctgt atatgtacaa cggcagcagc
     2221 aactcctatc ccctgtatag catcgagctc gaccgcgcca ccctccgtcc catcggcacc
     2281 cgccaggaga cctaccggct cgaagactgg cgctatggct ggcagcggtt tggcgaatac
     2341 tacgacaata ccttcctcga cccctttatc gaaggctcct ggatgaccaa acacaacggg
     2401 aaatattacc tccagtatgg cgcccccggc accgaattca gcggctatgc ggatggggta
     2461 gtagtgggcg accggcccct gggcaaccag tggacccccc agtccgaccc cctttccttc
     2521 aaaccgggcg gctttgcccg cggcgccgga catggcgcca ccttccagga cctgctgggt
     2581 aactactggc acgtgagcac catcaccatc gccgtaaaga acaacttcga acggcgcatc
     2641 ggcatctggc ccgccggctt cgacaaggac gacgtgatgt actgcaacac aagctatggc
     2701 gactatcccc acttcctgcc cgcagaaaga acggcggaat ataatgcccc gaccggcaac
     2761 ggcggcttca caggctggat gctcctcaac tataacaaac cggtacaggt gtcgtccacc
     2821 actggcgcct tcacggccaa cttcgccgtc gatgagaaca taaagaccta ttggagcgcc
     2881 gctaccggtg acccgggaga atggatccgg accgatctcg gaagactgtc caccgtcaat
     2941 gcgatccagg tcaactacgc cgaccaggac gccgtcttcc tcggcaagca aacggatatt
     3001 tatcatcaat atgttctcta cgcctccacc gacgggaaga aatggaccgt cctggtggat
     3061 aaaagcagga acgggaccga cgtaccccat gattatatag aactcgaaca ccccgtacag
     3121 acgaggtata taaagatgga gaaccgccat atgccctcgg ggaaatttgc catcagcggt
     3181 ttgcgcgtct ttggcaacgg cggcggcgaa agaccggatt ccgtaaaaca gtttatcgtc
     3241 ctgcgcaccg aaaaagataa gcgcagcgcc tggatcaagt ggcggccgat agacaatgcc
     3301 tacgcatata acatttattt cggcaccgat ccgggcaaac tgtacaacag catcatggtc
     3361 tataacgcca acgaatattg gtttaaggtc atggacagta aaaagcccta ttatttttcc
     3421 atagaagcct tgaacgagaa cggggtctcc ccgcgcacca cgatagtaaa aatcaactaa
     3481 atgaccattc aaatatgaaa gcagcaaaaa cgattattgc ttttctcctt ttcgccgcca
     3541 tcaccgcccg gggccaggcg cctaccgccc gcgcccaggc gcctaccgcc ggcacggacg
     3601 ccaaaatgaa cacattcgtc tccaccctga tgggcaggat gaccctcgat gaaaagatcg
     3661 gtcagctgaa cctccccggc gccggcgata tcactacagg acaggccggc aattccaaca
     3721 tcgccaaaaa gatagaacag ggcctcgtcg gcggcctctt taatatcaaa tcggtcgaaa
     3781 aaattaagga agtacaacgc atcgccgtcg aaaagagccg gctgaagatc ccgctcatct
     3841 tcgggatgga tgtcatacac ggctatgaga cggtattccc catccccctg ggactgagct
     3901 gtagctggga tatggcggaa attgaaaaaa gcgcccgcgt cgccgcaacg gaagccagcg
     3961 cggacggcat ctgctggacc ttctccccca tggtcgatat cgccagggac ccccgctggg
     4021 gacgcatcgc cgaaggcaac ggcgaagacc cctacctggg ctcacagatc gcaaaagcaa
     4081 tggtcaaagg ctaccagggc gatctttccg gcaagtcgga tatcatggcc tgtgtcaaac
     4141 acttcgccct ctacggcgcc gccgaagccg gccgggatta caacacaacg gatatgagcg
     4201 agatccggat gttcaatgaa tacttccctc cctaccaggc cgccgtcgac gcgggcgccg
     4261 ggagcgtaat ggcctccttc aacgacgtca acggcacccc cgccacggcc aacaagtggc
     4321 tgatgaccga tgtcctccgt catcgctggg gctttaaagg atttgtcgtc accgactaca
     4381 cgggtctcaa tgaattgatc ccccatggcc tcggcgatct gcaaacagtc tccgccctcg
     4441 ccctgaaggc cggtatcgac atggatatgg ttggcgaagg attcctcacc acgctgaagg
     4501 tatccctgca ggaaggaaag atcaccctgg cggagatcga caaggcttgc cgaagagtac
     4561 tcgaagcaaa atataaattg gggctgtttg gcgacccgta caaatactgc gacgaaaaaa
     4621 gagccgctac cgagatcttc accgacgcca accgctccac ggcaagacag atcgccgccg
     4681 actgtttcgt gttgctgaag aaccagggca acctcctccc gctgaagaaa ggcgcgacga
     4741 tcgcgctggt gggaccgctg ggagacaaca aggagaacat gccgggaacc tggagcgtcg
     4801 ccgccgactt ctccaaatcg atctccctcc ttcagggaat gaaagacgtg gccggcgacg
     4861 gaacaaagat cctctatagc atgggctcca acctcgacgc cgacagcctc ttcgaagtcc
     4921 gcgcggggat gtttggcaaa gccctgcatc acgactcacg tcccgccgcc gacattattc
     4981 acgacgccct cacggtcgcc ggtcaggccg atgtcatcgt agccgccctc ggcgaatcgg
     5041 ccgagatgag cggcgagtcc agcagccgta gccatatcga tctcccccag gcacagcagg
     5101 atctgctaaa ggccctgctg gcgaccggta aacccgtcgt actggtcctc ttcaccggac
     5161 ggcccctcgc catcaaatgg gagaacgacg ccactcccgc tatcctgaac gtctggttcg
     5221 gcggctccga agccggtcac gctatcgccg atgtgctgtt cggcacggtc aacccctccg
     5281 gcaaactgac caccacctgg ccgcagaacg taggccaggt acccctttac tataatcaca
     5341 tgaataccgg ccgccccctc gaaggccact ggttccagaa gttccgctcc aactacctcg
     5401 acgtttccaa cgaccccgtc ttcccctttg gctacgggtt gagctatacg cattttagct
     5461 acagcgaggt aaccgtcagc agcaaaaacc tgaaaggcga ccaaaccctc agtgcgacgg
     5521 tcactgtgac gaatgacgga caggtacagg gaaaagaagt ggtgcagctc tatatccgcg
     5581 acctggtagg cagcatcacc cggcccgtga gcgaattgaa gggttttcaa aagatcgacc
     5641 tcaaacccgg ggagtcccgt caggtgacct tctccatcac cccaagggat ctgaaatttt
     5701 acaacgccga tctcaaatac gactgggagc ccggtgattt cgtcatcatg atcggcggca
     5761 attcccgcga tgtgcgatcg gcaaaaatta actggacgaa gtaacaataa catgagaacc
     5821 cccc
//
LOCUS       DATGFG010000335         5073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1070103, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000335 DATGFG010000000
VERSION     DATGFG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070103"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VKQ52_06300"
     CDS             complement(<1..809)
                     /locus_tag="VKQ52_06300"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86826.1"
                     /translation="MLPFAACKKDPYGSGQAQNELVVLAEITADYPLKIPVSKSIQVG
                     NGGVITFDKVSTANVTISADNGSPWLCNLNTSPDYSGDVASVYTSPQRPRPNMTYHLK
                     VQDPLSGTATAQTTIPSRPHLVSYDTSGDMRGGQPVLKLRVTLKDPPDTSNLYVFEAL
                     KELLRIQHLFTYQGKTYNYDTDAGKKLYDQVKGQPGVRLRTDTVSTNKFLRLSLYTDD
                     RNVDNAQVSSLDSPFARIFLPDKVFNGEAYTETIYVDRTYFRATGNAGPGRV"
     gene            complement(877..3483)
                     /locus_tag="VKQ52_06305"
     CDS             complement(877..3483)
                     /locus_tag="VKQ52_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ86827.1"
                     /translation="MRPCRRYLLLPVIFSFLAMPLVAQKKGVLYRPFHSQIQKGTIGD
                     FLNEINARSGIIVEYATGSFSLDKEVSVGGDIVTLGALLQTILAGQPVKAIEKNDKLI
                     LVPSPVPLPEDALLPQYSVFGIVREEGSRESMADAVVWEAATRKGAICNIHGYFSLSL
                     PEGKHRIEITHAGYKPLVIDLDLHWDQRTDVSLSPREDNPEVIVSGNRAPKKDGRETI
                     LPGQYDAYTNFLGENDAIRALYILPGVTNVADAASGLLVRGGEQDENLFLLDGNPIFN
                     PTHMLGALSIVDKTSLKAIRFFKSDFPSRFGGGLSSVIDVYTKDGNMQKWGGETNVNF
                     LAVSTTAEGPLQKDRTAMMASFRHSMPNYILNLFEKDFFSNFYDAHFKITHLLNRNDK
                     LTMNVYSGEDNLNLQTNNQNLNNSQRWGNRMASVGWTHVLGSQSFVTTSVNASQYYNL
                     AGFIYTVYNDSTGAPVSNRSFTTYSSISQFNLKSQFELIASNHLTLKYGGEFALARIK
                     PFESRVDSTISTDPSSFQSSVPLPYRQFDVYAEGEIRAGTRLLLRPGMHLSSFHFRDF
                     HFNSFQPRLYAAYRVGGSHQLYVSYNKMTQYLHLVTNPSLGINSDLWVPSTAALRPEE
                     SQTIDLGYSFRRKGKYSLAADVYWKEMRNVTNYAEGKSFFINTDSAWEQNIEAGKGRS
                     YGLEVMAEKEGQKLSVHLSYALSWNWRQFDQINKGKAFPFKYDRRHVLNLATTYRLDK
                     RMDISALWMFATGDVYSLPERIYPDYDNVQQIVNPGDLLRDYRFIYHFSGVNQYRTPP
                     YSRFDLSASYHPPRKKRFGFNWTAGIYNVFGSPSQYSYGLAGTLHSKSIIIISKDKLF
                     NVTPYISTTIDY"
     gene            complement(3480..4529)
                     /locus_tag="VKQ52_06310"
     CDS             complement(3480..4529)
                     /locus_tag="VKQ52_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ86828.1"
                     /translation="MKNTDPNMEGLILKQLRREASPQEQGELQDWLDADAANREEYEA
                     LAALWDVSGDVLQARKFDGAAAWSSMRERMAGGAKMEMGSELAAGSDGQEELTVGSKT
                     VHRPWRRYWMAAAAVLLVLCGAGGWLFYRGIRLSTTSYVAAGANRSILLPDGSRVWLR
                     KGSTLQYTATASGVRRVVNLKGGAYFEVAHDPANPFVVKTPRSTIEDVGTSFIVLQES
                     QVEKVIVTAGSVRVRAGAPSTQGIVLTAGEKVVVTENGVTRGRQTDPNFLSWKTNVLD
                     FNGTPLDEVAADIHDLYGIPVGLSPDVQAEAGRIRVTAKFPHSEDIKEVLDELKLMTG
                     LSIQQEKDTLIFFRK"
     gene            complement(4567..>5073)
                     /locus_tag="VKQ52_06315"
     CDS             complement(4567..>5073)
                     /locus_tag="VKQ52_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HLZ86829.1"
                     /translation="AVFATLYGPLCQYALTYIKKPEICEDIVQEVFARVWEKRKDLLL
                     SDSIRFYLFTAVRNNCISHLRKEKTAGDVGLNGLETEHYPVVLPMEGLGPGDQHRLLA
                     EAINELPGKCREIFVLSRLSNMKYKEIADALGISIKTVENQLGKALKMMRRFLQKKGI
                     ETYSSSRE"
BASE COUNT         1094 a         1540 c         1338 g         1101 t
ORIGIN      
        1 accctgccgg ggcccgcatt gcccgttgcc cggaaatagg tccggtccac atagatcgtc
       61 tcggtatagg cctccccgtt aaagaccttg tccggcagga agatgcgtgc aaaaggactg
      121 tccagcgaac tcacctgcgc attatccaca ttccggtcat cggtgtacag ggacagccgc
      181 aggaatttat tcgtcgagac cgtatcggtg cgcaaccgca cccccggctg acccttcacc
      241 tggtcgtaca acttcttgcc cgcgtccgta tcatagttgt aggtcttccc ctggtaggta
      301 aaaagatgct ggatgcgcaa cagctccttc agcgcctcga aaacatagag gttactggtg
      361 tcgggcgggt ccttcagtgt aacccgcagt ttgagcaccg gctgaccacc ccgcatgtcc
      421 ccgctggtat catagctgac gaggtgcggc cgggaaggga tggtcgtttg cgccgtcgcc
      481 gttccgctaa gcgggtcctg caccttcagg tgataggtca tattgggcct gggccgctgc
      541 ggactcgtat agacggaggc cacgtcaccg gaataatccg gggaagtatt caggttgcag
      601 agccaggggc tgccgttatc cgccgagatg gtcacattgg cggtcgacac cttgtcgaag
      661 gtgatgaccc cgccgttgcc cacctgtatg ctcttgctga cagggatctt gagcggatag
      721 tccgcggtga tctccgccag caccaccagc tcgttctgcg cctgccccga cccataaggg
      781 tccttcttac atgcggcgaa cggcagcata caacccgcaa cgacaagccc cggcaaccaa
      841 cccgcccggc gcccaccccc ggcgcgagcc tcccccctaa taatcgatcg tggtcgaaat
      901 atatggcgta acattgaaca acttatcttt tgaaatgatg atgatgctct tgctatgcag
      961 ggtccccgcc agcccataac tgtattggct gggcgacccg aacacattgt atatcccggc
     1021 cgtccagttg aacccgaatc gcttctttcg tggggggtga taactggcgg aaagatcgaa
     1081 ccgggaataa ggcggcgtac ggtactggtt gacccccgaa aaatgataga tgaaccggta
     1141 atcccgcaac aggtccccgg gattcactat ctgctggaca ttatcatagt ccgggtagat
     1201 ccgctcgggg agggaataca catcccccgt ggcgaacatc cacaacgcgg aaatatccat
     1261 gcgcttatcc agccggtagg tcgtcgcgag gttcaggaca tggcgccggt catatttaaa
     1321 agggaatgcc ttccccttgt tgatctgatc gaattgccgc cagttccagg acaaggcata
     1381 ggagagatgg acgctgagct tctgaccttc tttctccgcc atcacttcca ggccatagct
     1441 ccggcccttg cccgcctcga tgttctgctc ccaggccgaa tcggtattga taaaaaaact
     1501 cttcccctcc gcataattgg tcacattgcg catctctttc caatacacat ccgccgccaa
     1561 cgagtatttc ccctttcgtc tgaacgaata gccgagatcg atcgtctggc tttcttccgg
     1621 ccgaagcgcc gccgtactcg gcacccaaag atcgctgttg atacccaggg atgggttggt
     1681 cacgagatgc aggtattgcg tcatcttgtt gtaggagaca tacaattgat ggctcccgcc
     1741 tacacggtaa gcggcgtaaa gcctgggctg aaaggaattg aagtgaaaat cccggaaatg
     1801 aaacgaagag agatgcatcc ccggacgaag cagcaaacgc gtgcccgccc ggatctcgcc
     1861 ctcggcatag acatcgaatt ggcggtaggg taggggaacc gacgactgga aggaagacgg
     1921 gtccgtcgag atggtcgaat ccacccggct ttcgaacggc tttatcctgg ccagcgcaaa
     1981 ctcgccgcca tacttcagcg tgagatggtt ggaggcgatg agctcgaact ggcttttcag
     2041 attgaactgg ctgatggagg aataggtagt gaacgaccgg ttgctcaccg gcgcacccgt
     2101 ggagtcattg tacacggtat agatgaaccc ggccaggtta taatactggc tggcattcac
     2161 ggacgtagtg acaaaggatt gggaacccag cacatgcgtc cagccgacag aggccatccg
     2221 gttgccccag cgctggctgt tgttcaggtt ctggttgttg gtctggaggt tgaggttgtc
     2281 ctccccggaa taaacgttca tggtcagctt atcgttccgg ttgagcagat gggtgatctt
     2341 gaaatgcgcg tcatagaaat tgctgaagaa gtccttctcg aacaggttga ggatataatt
     2401 gggcatgctg tgccggaacg aggccatcat ggccgtcctg tccttttgca gaggcccttc
     2461 cgccgtcgtg gacacggcaa ggaaattgac attggtctcc ccgccccatt tctgcatatt
     2521 cccgtccttc gtatacacat cgatgacgga ggaaagaccg ccgccaaacc gggaagggaa
     2581 atcgctcttg aagaacctga tcgcctttag cgaggtcttg tccacgatcg ataaggcccc
     2641 cagcatatgg gtagggttaa aaataggatt tccgtcgagc aggaacaggt tctcatcctg
     2701 ttcgccccct cttaccagca acccgctggc cgcatcggcc acattcgtca cccccggcag
     2761 aatatacaac gccctgatcg cgtcgttctc gccaagaaaa ttcgtatagg catcgtattg
     2821 acccggcagg atcgtttccc tgccatcctt cttcggcgcc cggttacccg aaacgatcac
     2881 ctcgggatta tcctcccttg gagacagcga tacatcggtc cgctgatccc agtgcaggtc
     2941 caggtcgatg accagcggct tataccctgc gtgggtgatc tcgatccggt gtttcccttc
     3001 cggcagggac aggctgaagt atccgtggat attgcaaatg gcgcctttgc gggtggctgc
     3061 ttcccagaca acagcatcgg ccatcgattc ccggctgcct tcttcccgca cgatgccaaa
     3121 aacagagtat tgcggcaaca gcgcgtcctc gggcaggggc accggtgacg gcacgaggat
     3181 gagcttgtca ttcttttcga tggctttcac cggctgtccg gcgaggatcg tctgcagcag
     3241 ggcgccaagc gtgacgatat cgccgcccac gctaacctcc ttgtcaaggg agaaactgcc
     3301 ggtggcatat tccacgatta tcccgctgcg ggcattgatc tcgttgagaa aatcaccgat
     3361 ggtccccttt tgtatttggg aatggaaagg gcgatacagg acgccttttt tctgggcgac
     3421 aaggggcatt gccaggaagg agaagatcac cggcaataaa aggtatcggc ggcaaggtct
     3481 catttacgaa aaaagatgag ggtgtctttt tcctgttgta tcgagagacc cgtcatcaat
     3541 ttgagctcat cgagcacctc ctttatgtct tccgaatgcg gaaacttcgc ggtgacccgg
     3601 atgcggcccg cttccgcctg aacatccggc gacagcccga cgggtatccc atacaggtca
     3661 tgaatatcgg cggccacttc atccagcggc gtgccgttaa agtccaggac attggtcttc
     3721 caggacaaaa agttcggatc cgtctgcctg ccccgcgtaa ccccattctc cgtcacgacc
     3781 accttctcgc ccgccgtcag tacgatgccc tgcgtcgacg gcgcgcctgc cctgaccctc
     3841 accgaacccg ccgtgacgat caccttctcc acctggctct cctgcaggac gataaaactg
     3901 gtgcccacat cctcgatggt cgaccggggc gtctttacga caaacgggtt ggccgggtca
     3961 tgcgccacct cgaaataggc ccctcccttc aaattcacta cgcgccggac gccggaagcg
     4021 gttgccgtat actggagggt cgaccctttc cgcagccaga ccctggaacc atcgggtaat
     4081 aaaatagaac gattagcccc ggcggccaca taggaggtcg tcgacagccg gatcccccgg
     4141 taaaacaacc acccgccggc cccgcacaaa acgagcaata ccgcggccgc cgccatccaa
     4201 tacctccgcc acggccggtg cacggtcttc gaccccaccg tcaactcctc ctgcccatct
     4261 gaccccgccg ccaactcact ccccatctcc atcttcgccc cccccgccat cctctcccgc
     4321 atggaactcc acgcggccgc gccatcgaac ttccgggcct gcaggacatc cccgctgaca
     4381 tcccaaaggg ccgccagcgc ctcatattcc tcgcggttgg ccgcatcggc atccagccaa
     4441 tcctgcaatt cgccctgctc ctgcggggaa gcctcgcggc gcaactgctt caggataagc
     4501 ccttccatat tcggatcggt gtttttcatg ttatacttta taaaaacgcg attttggttt
     4561 ttacccctac tcccgggagg aagaataggt ttcgatcccc tttttctgca ggaaccggcg
     4621 catcattttt aatgccttac ccaattggtt ctcaaccgtc ttgatggaaa tgccaagcgc
     4681 atcggcaatc tccttgtact tcatattgct aagccggctc agcacgaaga tctcccggca
     4741 cttcccgggc agctcattga tcgcttcggc cagcagtcga tgctgatcgc ctggacccaa
     4801 accctccatc ggcaggacga cagggtagtg ctccgtctcc aggccattca accccacatc
     4861 gccggcggtt ttttccttcc ggagatggct gatgcaatta tttctgacag cggtaaaaag
     4921 atagaaccgg atggaatcac tcagcagcag gtcctttcgc ttctcccaga cacgggcaaa
     4981 tacttcctgc acaatgtctt cacagatctc cggctttttt atataggtga gggcatattg
     5041 acaaagcgga ccgtaaagag tggcaaaaac tgc
//
LOCUS       DATGFG010000336         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_713254, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000336 DATGFG010000000
VERSION     DATGFG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713254"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VKQ52_06320"
     CDS             <1..452
                     /locus_tag="VKQ52_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HLZ86830.1"
                     /translation="IHAKIAAQLGRRPIGRVENHHNFAWKEIVDGRECIVHRKGATPA
                     GKDVLGIIPGSMTASGYIVKGRGETASLSSAAHGAGRKMSRTAAMNSITHELMKKTLE
                     KHGVKLLGGGLDEAPQAYKDIEEVMRCQRQLVDVVGTFIPKIVRMDG"
     gene            complement(513..1430)
                     /locus_tag="VKQ52_06325"
     CDS             complement(513..1430)
                     /locus_tag="VKQ52_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ86831.1"
                     /translation="MMARIGRILLRTVVGLFIFLIILLLVFDRLVQFRMDDKELSSWF
                     HERQLEPKVSYYEAKGRKVRYLSIGDNPDASVLFIHGAPSSLSYWKSYLADSVLLSRA
                     TMYAVDRPGYGYSGLADPLPDISAQAGVIRLILDSLHKAHHPIIVVGVSYGSPIACRL
                     AMDYPELVDGLVLVAPPLGPGREKIFWFTYPVENPLIHWVVPRMLQTANQEKIHHKEE
                     LTKMLPLWERIHVPVIYLQGDKDGLVDTSNAGFAREHMTNAPSLDIRMIPGRGHLIAF
                     AEKDRIEKAIIELLDTTEAKRSKARPAGK"
     gene            complement(1470..2741)
                     /locus_tag="VKQ52_06330"
     CDS             complement(1470..2741)
                     /locus_tag="VKQ52_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ86832.1"
                     /translation="MNAASGSSPQQLVDHLFRHEAGKMIAVLTRIFGIHNLELVEDTV
                     QETFLKALQVWKFGAMPDNPSAWLMQVARNRTVDLIRRQQKFADISTELSMRLQSDTE
                     NTIQQFFLDTEIADSQLRMIFTCCHPGLNPEDQVALTLKTVSGFGVQEVAKALVSNET
                     AIQKRLYRAKEYIRTREIRFEIPTGQALNDRLETVYTVLYLLFNEGYNSSKADELIRH
                     DLCAEAMRLCKLLTEHRVTAQPSGFALLSLMCFQASRFESRLGEDNTIILLHQQDRRK
                     WDRRLIQMGYHYLNGSSTGDLLSVYHIESAIAAEHCLAPDFGSTNWGEMLHLYDLLLE
                     RKPAPIVKLNRAIVLAQTAGVVAAIDTILEIPGIAGLLEQHYIYAAVLGDLYGRAGNV
                     PEARRLLEQAYALTPSLAEKKLLQEKIAFFA"
     gene            complement(2751..3095)
                     /locus_tag="VKQ52_06335"
     CDS             complement(2751..3095)
                     /locus_tag="VKQ52_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLZ86833.1"
                     /translation="MKDFMFVFRGPYYEDLNFTPEQSQAAMQKWMEWIGQLSAKGIYV
                     GGAPLVREGKMVQGKKPLITDGPFTEGKETVGGYLIIKAESLKEATALALGFPDFELD
                     GTVEVREIIPMS"
     gene            complement(3172..3765)
                     /locus_tag="VKQ52_06340"
     CDS             complement(3172..3765)
                     /locus_tag="VKQ52_06340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLZ86834.1"
                     /translation="MSIRHFFFLLPIVVGLAACHSSNPDKPHVVIRTPAGDIEVELYP
                     RQAPKTAGAFLSYVDSGFYKNASFYRVLNEDNQVTGSPVSNLIQGGIWKTNHARAVSL
                     PGVPHEPTRDTHILHTDGVISLARDKPGTGTSEFFICVGDQPGFDFGGENNPDGQGYA
                     AFGKVISGMDVVRTLYARPESNQAFTPQIAIYTIVRK"
     gene            3805..4464
                     /locus_tag="VKQ52_06345"
     CDS             3805..4464
                     /locus_tag="VKQ52_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HLZ86835.1"
                     /translation="MHFDLPFLHTIFIGFLSLFPAVNPVGSAFIIDPLLGDLSYPERK
                     RAAKKVALYCFAICIVSALIGSWIMRLFGISIPIVQLAGGVLICRMGWTLLTTDNVKG
                     EKETAQPVRHPGDIDNLLFYPISFPMSTGAGTISVILTLAAHEDETDLLNHVLNLGAL
                     FVAILLICLLIYICYANTARLIHRLGPRGEQIVNRLSAFLVFCIGMQIAWSGIRALFK
                     I"
BASE COUNT         1050 a         1394 c         1112 g          937 t
ORIGIN      
        1 tcattcatgc caagatcgcg gcgcaactgg gccgccggcc catcggccgc gtggagaacc
       61 accacaactt cgcatggaag gaaatagtcg atggacggga atgcatcgtt caccggaaag
      121 gggcgacacc cgccggaaaa gatgtcctgg ggatcatccc cggctcgatg accgcatccg
      181 gctatatcgt gaaaggacga ggggagaccg catccctctc ctccgctgcg catggcgcgg
      241 gacgaaagat gagccgcacc gcggccatga attcgatcac ccatgagctg atgaagaaga
      301 ccctcgaaaa acacggggtg aaactcctgg gtggtggcct cgacgaagcg ccgcaggcct
      361 ataaggacat cgaagaagtg atgcgatgcc agcggcaact ggtcgatgtg gtgggtacat
      421 tcatcccaaa gatcgtccgc atggacggct gaggggccac ttgcccgcat acatggctga
      481 caggccactt gccggcatgg acggctgaca ggctacttgc cggccggcct cgccttgctg
      541 cgcttggcct ccgtcgtatc cagtaactcg ataatagcct tttcgatcct atccttttcc
      601 gcaaaagcga tcaaatgccc ccggcctggt atcatccgga tatccaggga gggggcattg
      661 gtcatatgct ccctggcaaa accggcatta ctcgtatcca caagcccatc cttatcccct
      721 tgcaggtaaa taacgggaac atggatacgc tcccataaag gcaacatctt cgtcaactcc
      781 tccttatgat ggatcttctc ctggttggcc gtttgcaaca tccgcggaac aacccaatgg
      841 atcaacgggt tctccacagg ataggtgaac cagaagatct tctcccgccc cggcccaagc
      901 ggcggcgcca ccaaaacaag cccatcgacc agctccgggt aatccatggc cagccggcag
      961 gcgataggcg aaccatagga gacacccaca acgatgatgg gatgatgcgc cttgtgcagg
     1021 ctatccagga tcagcctgat aacacccgcc tgggcgctga tatccggtaa agggtcggcc
     1081 aacccgctat atccataccc cggtctgtcc acggcataca tcgtggccct cgacaataat
     1141 acgctgtcgg caaggtaact tttccagtaa ctaagagagc tgggcgcgcc atggataaaa
     1201 aggacagaag catcgggatt gtcaccgatc gagagatagc gcaccttccg gccttttgct
     1261 tcgtagtagc tgaccttggg ttcaagctga cgttcgtgga accatgagga caattctttg
     1321 tcatccatcc taaactgcac cagccggtcg aacacaagaa gaaggatgat aagaaaaata
     1381 aataacccca cgactgtccg gagtaagatc cggccgattc ttgccatcat acaatacaaa
     1441 taacggttat atcctatccg gcaataaaat taggcaaaaa atgctatctt ctcctgtaac
     1501 aatttctttt ccgccagcga cggggtcagt gcataggcct gctccagcaa ccgcctcgcc
     1561 tcggggacat tccccgcccg cccatacaga tcgcccaata cggccgcgta aatataatgc
     1621 tgctccagca aacccgcgat cccggggatc tcgaggatcg tatcgatcgc cgccaccact
     1681 cccgccgtct gcgcgagaac gatcgcccgg ttcagcttca cgatcggcgc cggcttccgc
     1741 tccagcaaaa gatcatataa atgcaacatc tccccccagt tggtcgaccc gaaatcgggc
     1801 gcaagacaat gctccgccgc gatagccgac tcgatatgat aaaccgataa caggtcccca
     1861 gtggaggagc cgttgagata atgatacccc atctgtatca gccgccggtc ccatttccgg
     1921 cggtcctgct ggtgcagtaa gatgatcgta ttgtcttcac caagccggct ctcgaaacgc
     1981 gaggcctgga aacacatcag cgagagcaac gcaaaccccg agggctgcgc cgtcaccctg
     2041 tgctcggtca gcaatttgca aagccgcatc gcctccgcac agagatcatg ccggatcagc
     2101 tcatcggcct tcgaagaatt ataaccctca ttgaataaca ggtataacac ggtatacacc
     2161 gtctccagcc ggtcgttcaa cgcctgcccc gtcgggatct cgaaccggat ctcacgcgtc
     2221 cggatatatt ccttcgcccg gtaaagccgc ttctggatgg cggtctcgtt ggagacaagc
     2281 gccttggcaa cctcctgcac gccaaaaccc gaaaccgtct tcagggtcag cgccacctgg
     2341 tcctccgggt tcagcccggg atgacaacag gtaaagatca tccgcaactg gctgtccgcg
     2401 atctccgtgt cgagaaagaa ctgctgaatg gtattttccg tatcggactg cagccgcatc
     2461 gacaactccg tggagatatc cgcaaacttc tgctgccgcc ggatcagatc gacggtccgg
     2521 ttgcgggcca cctgcatcag ccaggccgac ggattgtcgg gcatcgcccc aaacttccag
     2581 acctgtaacg cctttaaaaa tgtttcctgc accgtatctt ccaccaactc gagattgtgg
     2641 atgccgaaga tccgcgtcaa aacggcgatc atcttccccg cctcatgacg gaaaagatga
     2701 tcgaccaatt gctggggcga agagccggat gccgcattca tagcaaaaaa ctaagacatg
     2761 ggaatgatct cgcgcacttc caccgtccca tcgagctcaa agtcggggaa gccaagagcc
     2821 agagcggtag cctccttcag ggattcggcc ttgatgatca ggtagccgcc gaccgtctcc
     2881 tttccttcgg tgaaagggcc atccgtgata agcggcttct tgccctgtac catcttgcct
     2941 tcccgcacga ggggcgcacc gccgacataa atacccttgg cgctgagctg cccgatccat
     3001 tccatccatt tttgcatggc cgcctgggat tgctcgggcg tgaaattcag gtcttcgtaa
     3061 taaggaccgc ggaaaacgaa cataaagtct ttcatgataa ctttctttaa aggttaaaac
     3121 tgatttatcc tttgacgaac gacaaaaaac agatcggaca attttcccgg attattttct
     3181 gacaatagta taaatcgcta tctggggtgt aaaagcctgg ttgctctccg gccgggcgta
     3241 aagcgtccgc accacatcca tcccgctgat taccttccca aaagcggcat acccctgtcc
     3301 gtcagggtta ttctcgccgc caaaatcaaa tcccggctgg tcacccacac aaataaagaa
     3361 ttccgatgtc cccgtcccgg gcttgtcccg cgcaagcgag ataaccccat cggtatggag
     3421 gatatgcgta tccctggtcg gctcatgcgg tacccctggc aggctgacgg ccctggcatg
     3481 attggtcttc cagatgccgc cctggataag attggagaca ggcgagcccg tcacctggtt
     3541 atcctcattc aacacccggt aaaaagaggc gttcttataa aaccccgaat ccacatagga
     3601 caaaaaggcc cccgccgtct tcggcgcctg ccgcgggtag agttcgacct cgatatcccc
     3661 cgccggtgtc cggatcacca catgcggctt gtccgggttg gaggaatgac aggcggcaag
     3721 tcccacgacg atcggaagta agaagaaaaa atgccgtatg ctcatggttg agtttgagag
     3781 tcacaattta acgtaatatt gtttatgcat tttgacctgc ccttcctgca taccatattt
     3841 atcgggttcc tctccctctt ccccgccgta aaccccgtcg gctcggcctt tatcatcgac
     3901 cccctcctcg gggatctcag ctaccccgag agaaaacggg ccgcgaaaaa agtggcgctc
     3961 tattgttttg ccatctgtat cgtctccgcc ctcatcggca gttggatcat gcggttattc
     4021 ggcatctcga tccccatcgt ccagctggca ggcggcgtcc tcatctgccg catgggctgg
     4081 acactcctca ccaccgacaa tgtaaaagga gaaaaggaga cggcccaacc cgttcgccat
     4141 ccgggggata tcgacaatct ccttttctat cccatctcct tccccatgtc caccggggca
     4201 ggcaccatct ccgttatcct gacgctcgcc gcccatgagg acgagaccga tctcctcaac
     4261 catgtcctca acctgggcgc cctgtttgtc gccatcctgc tgatctgcct cctcatctat
     4321 atctgctacg ccaacaccgc ccggctcatc caccggcttg gaccaagggg cgaacagatc
     4381 gtcaatcgcc tcagcgcctt tctcgtattc tgcatcggca tgcagatcgc ctggtccggc
     4441 atccgcgccc tgttcaagat ctgaccacac tctcctgttc aagctctgat cat
//
LOCUS       DATGFG010000337         4784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_292439, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000337 DATGFG010000000
VERSION     DATGFG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292439"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VKQ52_06350"
     CDS             complement(<1..657)
                     /locus_tag="VKQ52_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86836.1"
                     /translation="MLQNRVDPFGNIIRTPERGHWMGNRGLLHNEHQEIVRPFRLKAW
                     ITCVLAFKGRRRKVMAPGQYTELFFFDEATAFAAGHRPCFECRREDALRFKRYWLKGN
                     PSYGFDEKTLIREIDEVLHEERIAPDGSKVLFGERFDRLPNGSFISWKGEAYLVHDGF
                     VYRWTPGGYEAGVAWPEGVAASEGVAGPEGVVRPKGVATPEGVVEVLTPRSVVNAFRA
                     G"
     gene            954..1538
                     /locus_tag="VKQ52_06355"
     CDS             954..1538
                     /locus_tag="VKQ52_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLZ86837.1"
                     /translation="MSSEEQLIRRARTDPRAFGELYDRYYPKIFGYVFRATTDYAVAC
                     DITSETFLKAWIKLGSFKWKGISISSWFFRIATNEINQYFRRRSYTPHTVMEQSLLGL
                     SSWGNGAFPIDRSVNPYLRLEKLEEYRWIHTLLDRLPPIYRTVIVLRYFEDLGIREIA
                     EILDKKETTIRSLLFRGLERLKKLFPPAATNNTP"
     gene            1562..2026
                     /locus_tag="VKQ52_06360"
     CDS             1562..2026
                     /locus_tag="VKQ52_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86838.1"
                     /translation="MTQEKMENRLKDIPKPEPMPPAAQEMLRLTLLNAKRSSRVGLLL
                     IVLPGLVVLVFFFQNAFNTFPGLTRWFAHEATLIPMPLRAILLFLFLAGFPFIALVIN
                     MLAITYYRYNPLRREVTVIVKMKWNNILVAVFGCALATFYILHLLADTLLGK"
     gene            2049..2564
                     /locus_tag="VKQ52_06365"
     CDS             2049..2564
                     /locus_tag="VKQ52_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86839.1"
                     /translation="MRTRDRLLALVAMPLILLLPLACAQRSADTDALQAQLTRLQQRL
                     DSAYKPGLGEFMSGIQVHHEKLWFAGKAENWKLADFEIGEIKETIDDIRQFCASRPEV
                     ASLPMITPALDSLDAAIKNTSPTRFTSAFQFLTTTCNNCHKVTKHEFNVIKIPDTPPF
                     SNQVFTKPGEK"
     gene            2554..3768
                     /gene="chrA"
                     /locus_tag="VKQ52_06370"
     CDS             2554..3768
                     /gene="chrA"
                     /locus_tag="VKQ52_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HLZ86840.1"
                     /translation="MKNNTPPPATPTNGTSSGETSPGGASLPETSSGSTSSGGTSLRE
                     LAQLFTKLGFTAFGGPAAHIAMMQKEVVDKRGWMDHQHFLDLVGATNLIPGPNSTEMA
                     IHIGYERGKWKGLLVAGLCFILPAVLITATIAWLYQRYGRLPQVNTWIYGIKPAIIAI
                     ILAAAWPLAKRAVKTTQVGIIGALVFLAILLGVDEIIAMFAAGALTILLYLLRRPQKS
                     LSAVFPLTLLYINNPNLRIFLIFLKIGAILYGSGYVLFGFLDKELVATGLLQRAQLID
                     AIAVGQFTPGPVFSSVTFIGWQMGGWQGALTATIAIFLPSFVFVALSNPLLPRLRRSK
                     LFSAFLDGVNVASVAIIAAVCTSFGKAVLDDWRTILIAVLSTAFVFGLPKINSVFVVI
                     GGSLLGYILSLA"
     gene            complement(3806..4510)
                     /locus_tag="VKQ52_06375"
     CDS             complement(3806..4510)
                     /locus_tag="VKQ52_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ86841.1"
                     /translation="MNIRCLLVDDEPLAIQLLQKHLGQLPFFEVAGVCSNAVQALEVL
                     NHQAIDLLFLDIRMPQLSGLELLKNLRQPPPTILTTAYREYALDGFELDVVDYLLKPV
                     TFDRLFKAVEKFMRMNNRSPGPALSSSEPAFIYLKSGYKYFKIDLREILYAESRKDYI
                     RVVTKEKVIVSKYRLSDLEKELEGKGFVRIHRSYIVGLRHVVAFTASEVEVGEEQLPI
                     GESYRVFVERAVRRRS"
     gene            complement(4507..>4784)
                     /locus_tag="VKQ52_06380"
     CDS             complement(4507..>4784)
                     /locus_tag="VKQ52_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86842.1"
                     /translation="PPLLLLSLAENAFKHGASQDMGDPFIEISLEAGEDRLRYRVVNS
                     IPGAPAGNGSGKIGLSNLQKRLELLYPGRHYFYTGYDKNTFRAEVEL"
BASE COUNT         1116 a         1685 c          989 g          994 t
ORIGIN      
        1 ccccgcccga aaagcattca ccaccgacct cggcgtaagc acctccacca cgccttccgg
       61 cgtcgccact cccttcggcc tcaccacgcc ttccggcccc gccaccccct cagacgccgc
      121 caccccctcc ggccacgcca caccggcttc atatcccccc ggcgtccacc ggtaaacaaa
      181 accatcatgc acgagataag cctccccctt ccacgaaata aaacttccgt tcggtaaccg
      241 gtcaaagcgc tccccgaaca agaccttgct tccatccggc gcgatccgct cctcgtgcaa
      301 cacctcatcg atctcccgga tcaacgtctt ctcatcgaac ccataagaag gattcccctt
      361 tagccagtat cgcttgaacc gcagagcatc ctccctcctg cactcgaaac agggccggtg
      421 tcccgccgca aacgccgtcg cctcgtcaaa aaagaaaagc tcggtatatt ggccgggcgc
      481 catcaccttc cttctccgcc ccttgaacgc aagaacacag gtgatccagg ccttcaaccg
      541 aaaaggccgc actatttcct ggtgctcatt atgcaatagc ccccggttgc ccatccagtg
      601 cccccgctcc ggggtccgga taatgttccc aaatggatcg actcgatttt gtaacatgat
      661 cattccctcc tacgtagctc ataacgtaaa gatactgcat tgggtaaatt cgctagaggg
      721 aaatacctac cgatcgatag acgatttgag aacttcgatt gaatagtaga gaaaatgctt
      781 gcactccgga aaatgccgcc ttacctttgt agcaggataa ggtttaatgg ttaatggtta
      841 acgctatatg atttttagtt tttccccggc agcgccgggg ttttttattt aaaaaatacc
      901 cgccggctgc aacaatttat ccatcttagc attacacgga aaaattaaga cgtttgtcca
      961 gcgaagaaca gttgatccgg cgggccagaa ccgatcccag ggctttcggc gaattatacg
     1021 atcgctacta cccaaagatc ttcggttacg tattccgtgc aacgacggac tatgccgtcg
     1081 cttgcgatat cacctcggag accttcctaa aggcctggat aaagcttggc tcctttaaat
     1141 ggaagggcat ctccatctcc tcctggttct tccgcatcgc cacaaacgag ataaaccagt
     1201 acttccgccg ccggagctat accccccata ccgtgatgga acaatccctc ctgggactca
     1261 gctcctgggg caatggcgcc tttcccattg accggtccgt caatccctac ctccggttgg
     1321 aaaagctcga agaataccgg tggatccaca ccctgctgga ccggctcccc cccatttatc
     1381 gtaccgtcat cgtcctgcgc tacttcgagg acctcggcat ccgcgagatc gccgagatcc
     1441 tggacaagaa ggaaacaacc atccgctccc tgctgttccg tggactggaa agactaaaaa
     1501 aattgttccc cccggccgca acgaacaaca caccctaagc attatacaaa aaacacgcac
     1561 catgacacag gaaaagatgg aaaacaggct gaaggatatc ccaaagccgg agccgatgcc
     1621 cccggccgcg caggaaatgc tgcgcctgac cctgctcaac gccaaacggt cctcgagggt
     1681 gggcctgctg cttatcgtcc tgccgggcct tgtcgtcctc gtcttttttt tccagaatgc
     1741 cttcaataca ttccccggcc ttacccgatg gtttgcacac gaagccaccc tcataccgat
     1801 gcccctccgg gcgatactgc tcttcctctt cctcgcggga tttccgttta tcgccctggt
     1861 aataaatatg ttggccatca cctactaccg ctataacccc ctgcggagag aagtgacggt
     1921 catcgtaaag atgaaatgga acaacatact cgtagctgta ttcggctgcg cactggcaac
     1981 cttctatatt ctccacctcc tcgcggatac cctcctcggc aaatagcgat tttccttact
     2041 ttagcggcat gagaacaaga gatcgcttac ttgccctcgt tgctatgccg ctgatcctcc
     2101 tcctcccgct ggcgtgtgcc cagcgttcag cggataccga cgcgttacaa gcccagctaa
     2161 cccgcctcca gcaacgcctc gacagcgcct ataaacccgg gctcggcgaa ttcatgtccg
     2221 gcatccaggt ccaccacgaa aaactatggt ttgccggcaa ggcggagaac tggaaactgg
     2281 cggattttga aataggcgag ataaaggaaa ctatcgacga catccgccag ttttgcgcct
     2341 ctcgccctga agtagcctcc ctccccatga tcacccccgc cctcgacagc ctcgacgcgg
     2401 caataaagaa caccagcccg acccgcttca ccagcgcctt ccaattcctc accaccacct
     2461 gcaacaactg ccataaggtg acaaaacacg aattcaacgt cataaaaata ccggacaccc
     2521 cgccattctc caaccaggta ttcacaaagc caggtgaaaa ataacactcc cccgcccgca
     2581 acacccacaa acggcacctc ctcgggcgaa acttccccag gcggcgcctc cctgcccgaa
     2641 acttcctcag gcagcacctc ctcaggcggc acctccctgc gcgaactcgc gcaactcttc
     2701 acaaaactcg gcttcaccgc cttcggcggc cccgccgcac atatcgccat gatgcaaaaa
     2761 gaggtagtcg ataaacgcgg ctggatggac caccaacact tcctcgacct cgtcggcgcg
     2821 acaaacctca tccccggccc aaacagcacc gagatggcca tccacatcgg ctacgaacgc
     2881 ggtaaatgga aaggcctcct cgtcgccggc ctttgcttca tcctgcctgc cgtcctcatc
     2941 accgccacca tcgcctggct ataccagcgc tatggccgcc ttccccaggt caacacctgg
     3001 atctatggca taaaacccgc gatcatcgcc atcatcctcg ccgcggcctg gccactcgca
     3061 aaaagagccg tcaaaacaac ccaagtcggc atcatcggcg ccctggtctt cctcgcgatc
     3121 cttctcggcg tcgatgagat catcgccatg ttcgccgccg gcgccctcac catcttgctt
     3181 taccttctcc gtcgcccgca aaagtcgcta agcgccgtct tcccattgac actattatat
     3241 atcaacaatc ccaacctccg catcttcctc atcttcctaa aaataggcgc catcctctac
     3301 ggcagcggct acgtcctctt cggcttcctc gataaggaac tcgttgccac cggcctcctt
     3361 caaagagcac agttgatcga tgccatcgcc gtgggacagt tcacacccgg accggttttc
     3421 agttccgtca ccttcatcgg ttggcaaatg ggtggctggc agggcgccct caccgccacc
     3481 atcgccatct tcttaccctc cttcgtcttc gtagctctct cgaatcccct tcttcctcgc
     3541 cttcggcgct caaaactctt ctccgccttc ctcgacggcg tgaacgtggc ctccgtagcc
     3601 atcatcgccg ccgtatgcac cagcttcggc aaagccgtgc tcgacgactg gcgcaccatc
     3661 ctcatcgccg tccttagcac cgccttcgtc ttcggacttc ccaaaataaa cagcgtcttt
     3721 gtcgtcatcg gcgggtccct cctcggctat atcctatcgc tggcctaagc tgcgccgccg
     3781 caaactcacg cccccttacc cctttctaac tccgcctcct caccgccctc tctacaaaca
     3841 cccgatagct ctcgccaatc ggcaattgct cctcacccac ctccacctca ctcgccgtaa
     3901 acgccaccac atgccgcaac ccaacgatat aactccgatg gatacgtaca aacccctttc
     3961 cctccaactc cttctccaga tcgctcaacc ggtacttgga aacgatcacc ttctccttcg
     4021 taacaacccg gatataatcc ttccggctct ctgcatacaa gatctcccgc aggtctatct
     4081 tgaaatattt ataacccgac ttcaggtaaa taaaggccgg ctccgaagaa gacaacgccg
     4141 gacccggcga ccggttattc atccgcatga acttctccac cgccttgaaa agccggtcaa
     4201 aagtcaccgg cttcaacaaa taatccacca catccagctc gaacccgtca agcgcatact
     4261 cccggtacgc cgtcgttagg atcgtaggcg gcggctgccg cagattcttc aacaactcca
     4321 gccccgaaag ttgcggcatc cggatatcca ggaacaatag atcgatcgcc tggtgattca
     4381 acacctccag cgcctgcacg gcattcgaac acacccccgc tacctcgaaa aagggaagct
     4441 gcccgagatg cttctgcagc aactggatcg ccagcggctc atcatcgacc aacagacacc
     4501 ggatattcat agctcaacct ccgcacgaaa ggtgttctta tcatagcccg tataaaaata
     4561 atgcctgccc ggataaagca gctccaaccg cttttggaga ttcgacagcc cgatcttccc
     4621 agacccattt cccgcgggcg ccccgggaat agaattcacc acacgatacc ggagccggtc
     4681 ctcaccagcc tccaggctta tctcgataaa cggatctccc atgtcctggc tggccccatg
     4741 cttgaacgca ttctccgcaa gcgacaataa caacagcggc ggta
//
LOCUS       DATGFG010000338         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_597358, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000338 DATGFG010000000
VERSION     DATGFG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597358"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VKQ52_06385"
     CDS             complement(<1..348)
                     /locus_tag="VKQ52_06385"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HLZ86843.1"
                     /translation="MKRSAMAGWSIVLLCMALCATSMSYISHPWPERFAKPTVLIFCK
                     TNGYHHASIATGIVAIKKLGEENGFAVDTTADSRWFRKKILKRYAALIFLSPTGKVFG
                     PKEEEALKDYIHHG"
     gene            complement(371..790)
                     /locus_tag="VKQ52_06390"
     CDS             complement(371..790)
                     /locus_tag="VKQ52_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLZ86844.1"
                     /translation="MTKQLVAALSCAILMAACGGSGDKKATENKDSATPATTTPAPPA
                     GGTAAADKALDLIGASDCTTCHRLNKNAGGAAIGPAYSDVAAKYSPAADSTVDRLVKK
                     VISGGSGIWGTVPMTPHPALKPEDVKTMVTYILSLKQ"
     gene            complement(844..1899)
                     /locus_tag="VKQ52_06395"
     CDS             complement(844..1899)
                     /locus_tag="VKQ52_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLZ86845.1"
                     /translation="MQTIKGPGIFLAQFLGDQAPFNSLDSICKWAAGLGYIGVQIPSW
                     DSRVIDLQKAAESKTYADEIKGIVQSAGLQITELSSHLQGQLVAVHPAYDTLFDGFAP
                     AAVHGNPKARQEWAVQQLKYAAKASHNLGLDAHATFSGALLWPTVYPWPQRPAGLVET
                     GFKELAARWLPILDEFDRYGVDCCYEIHPGEDLHDGVTYEMFLEHANNHPRACLLYDP
                     SHFVLQCLNYLEYIDNYHERIRMFHVKDAEFNPTGKQGVYGGYQGWVERAGRFRSLGD
                     GQVDFKGIFSKLTQYDFKGWAVLEWECALKHPEDGAKEGAIFIKEHIIRVTEKAFDDF
                     AGTGSDEKFNRSVLGLK"
     gene            complement(1975..2619)
                     /locus_tag="VKQ52_06400"
     CDS             complement(1975..2619)
                     /locus_tag="VKQ52_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ86846.1"
                     /translation="MSRLSEKDGSKQAEWRTDPARSGKAGAMGDIGTHAAHLAEYISG
                     LKIKQLCADLSIMVEGRALDDDGNVLLRFDNGATGVLTASQVAAGEENALKIRVYGEN
                     GGIEWAQQEPNTLLVKWLDQPTQVYRAGMGYLGSYAKFNTRTPGGHPEGYLEAFGNLY
                     RDFAITLMARIDGATPPKEALDFPSVEDGVRGMAFIDNLIRSNESKEKWTSFTI"
     gene            complement(2620..3132)
                     /locus_tag="VKQ52_06405"
     CDS             complement(2620..3132)
                     /locus_tag="VKQ52_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ86847.1"
                     /translation="MNRKLRMGMIGGGRNAFIGAVHRIAANMDGLIELVCGAFSSHPD
                     NARASGKDLFLPEDRIYLSYEEMLDKESKLPADKKMDFVVIVTPNHLHFAPAMLALEK
                     GFHVVLDKPMTFSLSEAIQLKRKVEDTGLLLCLTHTYSGYPMVKQARQMIKEGVFGKI
                     RKIYVEYPQG"
     gene            3309..>3857
                     /locus_tag="VKQ52_06410"
     CDS             3309..>3857
                     /locus_tag="VKQ52_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ86848.1"
                     /translation="MSQIQTKKLFTASCLALLVTSLSFGIRAGILNMQGVHFHLDASQ
                     LSTIAATAFWGFPLAIIIGGFIVDSIGMKKLLVCAFVFHLAGILLTIFANGYWTLFLS
                     TLLIGIANGTVEAACNPLVASLFTNNKTTKLNHFHLWFPAGIVIGTLVVFALNNLLAA
                     GTADLPYWVSQVEVAVMLIPTLV"
BASE COUNT          694 a          927 c         1219 g         1017 t
ORIGIN      
        1 accatgatgg atatagtctt tcagagcctc ttcttcttta gggccgaaga ccttgccggt
       61 ggggctgagg aagatgaggg ccgcgtatct tttcaggatc ttcttacgga accatctgga
      121 gtcagcggtg gtgtcgacgg cgaagccgtt ctcctcgccc agttttttga tggccacgat
      181 gccggtggcg atgctggcat ggtggtagcc attggtcttg caaaagatca gcacggtcgg
      241 tttggcgaac cgttcgggcc agggatggct gatatagctc atgctggttg cacagagggc
      301 catgcaaagg agtacgatgc tccatccggc cattgctgat cttttcattg gacctgtttt
      361 tatgtcgttg ctattgtttc agggagagga tataggtgac catggttttg acgtcttccg
      421 gtttcagtgc cgggtgtggg gtcatgggta cggtgcccca gatgccgctg ccgccgctga
      481 tgactttttt tacgagccgg tcgactgtcg agtctgctgc cgggctgtat ttcgccgcga
      541 cgtcgctata ggccggcccg atcgccgcgc cgccggcgtt cttgttcagg cggtggcagg
      601 tggtacagtc gctggcgccg atgagatcga gggccttgtc ggcggctgcg gtgccgccgg
      661 cgggaggcgc cggtgtcgtg gtggccgggg ttgcggaatc tttgttttcc gtggcttttt
      721 tgtctccgga gccgccgcaa gcggccatga ggatggcgca ggaaagggcg gctactagct
      781 gtttagtcat gtttatttgc tttggtgcgt ttatttactt tggtgggtcg aagttatgca
      841 gagttatttc aaaccgagca cggaacggtt aaatttttca tcgcttccgg tgccggcgaa
      901 gtcatcgaag gctttttcgg tgacgcggat gatatgttct tttatgaaga tggcgccttc
      961 ttttgcgccg tcctcggggt gtttcagggc gcattcccat tcgagtacgg cccagccttt
     1021 gaaatcgtat tgtgtcagtt tgctgaagat gcccttgaaa tccacctggc cgtcgccgag
     1081 cgagcgaaag cggccggcgc gttcgaccca tccctggtaa ccgccgtata cgccctgttt
     1141 accggtggga ttgaattcgg cgtcttttac gtggaacatg cggatgcgtt cgtggtagtt
     1201 gtcgatatat tccaggtaat tgaggcattg caggacgaag tgcgagggat cgtagaggag
     1261 gcaggcgcgg gggtggttgt tggcatgttc gaggaacatc tcgtaggtga cgccgtcatg
     1321 aaggtcttca ccggggtgga tctcgtaaca gcaatcgacg ccataccggt cgaattcatc
     1381 gagaatgggc agccagcggg cggccagttc cttaaagcct gtttcgacga ggcccgcggg
     1441 gcgttggggc cagggataga cggtaggcca gagcagggcg ccgctgaagg tggcgtgggc
     1501 atcgaggccc agattgtgtg aggctttggc tgcgtatttc aattgttgaa cggcccattc
     1561 ctggcgggct tttgggttgc cgtgcaccgc ggcgggtgcg aagccgtcga aaagcgtatc
     1621 gtatgcggga tgcaccgcca ccagctgacc ctggaggtgg gaggacaatt cggtgatctg
     1681 caggcccgcg ctttggacga tgccttttat ctcgtcggca taggtcttgc tttccgccgc
     1741 cttttggaga tcgatgacgc ggctgtccca ggaggggatc tggactccga tatagccgag
     1801 accggcggcc catttgcaga tagagtcgag ggagttgaaa ggggcctggt cgccgaggaa
     1861 ctgtgcgagg aagatgcccg gtccttttat tgtttgcatg tgacggttat ttttatggaa
     1921 tgatcggttg acggtagttt tttacggaat gatcggtgga tgatgtgctt tgggtcagat
     1981 ggtgaaggaa gtccacttct ccttgctttc gttgctccgg atgaggttat cgataaacgc
     2041 catgccgcgg acgccgtctt ccaccgaggg gaaatcgagg gcttcttttg gcggggtggc
     2101 gccgtcgatg cgggccatga gggtgatggc gaagtcgcgg tagaggttgc caaaggcttc
     2161 gaggtaccct tccggatgtc cgccgggggt gcgggtgttg aatttggcat aggagcccag
     2221 gtagcccatg ccggcgcggt agacctgtgt aggctggtcc agccatttta ccaggagcgt
     2281 attgggttcc tgctgggccc attcgatgcc gccgttctcg ccatagacac ggatcttcag
     2341 cgcattctct tcgccggcgg ctacctgtga cgcggtgagg acaccggtgg cgccgttgtc
     2401 gaagcgcagc aggacattgc catcgtcgtc gagggcgcgg ccttcgacca tgatgctcag
     2461 atcggcgcag agttgtttta ttttcaggcc gctgatatat tcggcaagat gtgcggcgtg
     2521 ggtgccgatg tcgcccatcg cgcctgcttt gccggagcgg gcagggtcgg tgcgccattc
     2581 ggcctgtttg ctgccgtcct tctcggagag gcggctcagt cagccctggg ggtattcgac
     2641 atagatcttt ctgatcttgc cgaagacgcc ttcttttatc atttgccggg cctgtttaac
     2701 catcgggtag ccggagtagg tgtgggtcag gcagagtaaa aggcccgtgt cctccacttt
     2761 cctttttagc tggatggctt cggacaggga gaaggtcatg ggtttatcga ggacgacatg
     2821 gaagcccttt tcgagggcga gcatggcggg ggcgaagtgc aggtgattgg gcgttacgat
     2881 gaccacgaag tccatcttct tatcggcagg cagtttgctt tctttatcga gcatttcctc
     2941 ataggaaagg tagatgcggt cttccgggag gaaaaggtct tttccggagg cgcgcgcgtt
     3001 gtcgggatgg ctgctaaagg ctccgcaaac cagttcgatg agcccgtcca tattggcggc
     3061 aatgcgatga acggcgccga tgaaggcgtt cctgccgcct ccgatcattc ccattcgtaa
     3121 ttttctgttc atatcttgaa aagttgtctg caattttagg acttctttat taaagtaaga
     3181 tattatttaa tgaattcgat tgcttcttaa aaaaatagcg gtgtaaataa ccggcaaggc
     3241 tacatttatt aaaaataaaa aattacattt acattgtgtc cgactaacat atctaaaccg
     3301 atcttgccat gagtcaaatc cagaccaaaa agctatttac cgccagctgc ctggccctgc
     3361 ttgtaacctc tctttccttt ggtatccggg ccggtatcct gaatatgcag ggggtgcact
     3421 ttcacctgga tgcttctcaa ttatctacta ttgccgcgac ggctttctgg ggtttcccgc
     3481 tggccattat catcggcgga tttatcgtgg attccatcgg gatgaagaag ctgcttgtct
     3541 gcgcttttgt ttttcacctg gcgggtatcc tgctgacgat ctttgcgaat ggctattgga
     3601 cgctttttct ctcgaccctg ctgatcggaa ttgcgaacgg tacggtggaa gcggcgtgta
     3661 atccgcttgt agcttcgctg tttacgaata ataagacgac caagctgaat cactttcatc
     3721 tctggttccc ggcgggtatc gtgatcggga ccctggtggt gtttgctctg aacaacctgc
     3781 tggcggccgg tacggccgat ctgccgtact gggtatcgca ggtggaggtg gcggtgatgc
     3841 tgatcccgac gctggtg
//
LOCUS       DATGFG010000339         6108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1054751, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000339 DATGFG010000000
VERSION     DATGFG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054751"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VKQ52_06415"
     CDS             <1..471
                     /locus_tag="VKQ52_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86849.1"
                     /translation="NAAAQITAGDIGASQQGNVVTEDNVQPGTLNVGQLSACGVTINQ
                     YVTVTFNNSTYSQTSGSYLYYGSGNFESFSNPYMTFSVPGLSGTGTFSPSNFMLYTGN
                     YRIAAATDNTLRVNVTTFGAVNQYVIGTLGGNVYDSTSKTVYPLTGNFKLLRTN"
     gene            complement(485..1375)
                     /locus_tag="VKQ52_06420"
     CDS             complement(485..1375)
                     /locus_tag="VKQ52_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HLZ86850.1"
                     /translation="MQNISVIGAGTMGNGIAHVFAQKGFTVSLVDTSAHQLEKALKMI
                     GLNLDRQIAKGLLSEEEKKSTLQAITTHTGIPAGVPKADLVVEAATENTELKLNIFRQ
                     LDEHAPMGCILATNTSSISISKIASVTRRPAAVIGLHFMNPVPVMKLIEVINGYATDA
                     SVTRQMVTLAEKLGKTPCVVKDYPGFIANRILMPMINEAIYSLYEGVAGVNEIDTIMK
                     LGMAHPMGPLQLADFIGLDVCLSILRVLHDGFGQPKYAPCPLLTNMVTAGYLGIKSGE
                     GFYKYTAGSKELVVSARFAS"
     gene            complement(1394..2029)
                     /locus_tag="VKQ52_06425"
     CDS             complement(1394..2029)
                     /locus_tag="VKQ52_06425"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ86851.1"
                     /translation="MKKFVLYFLLLSAPFAVHAQFDKLKDSVVQLYGVIMTADSLKAI
                     PSVSIVVKGRNQGTFSNDQGVFSIVVLKGDQIEFTSVGYKPVLYSIQDTVRGNQISLI
                     QLMVVDAQYLPATIIAARPTREQFERDFVNTKVPDDDIEIARQNNDAAKRRILSRSLP
                     ANSGEAASTYLRQNAQRYYYNGQAPPQNIFNPLAWADFIQAWKRGDFRNNN"
     gene            2131..3231
                     /locus_tag="VKQ52_06430"
     CDS             2131..3231
                     /locus_tag="VKQ52_06430"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HLZ86852.1"
                     /translation="MTPEKVLAALGNVQEPDLGKDIVTLNMVKDLEINGNYVAFTVVL
                     TTPACPMKDHIKNACINAIKLLVNKEATVNVRFTANTTTQRKDAREMLPKVKNIIAVV
                     SGKGGVGKSTVAANFAIALARGGASVGLMDADIYGPSVPIMFGVRGQRPMMMDGGQNG
                     KGLIVPLEKYGIKLMSIGLLVDEKNAVVWRGPMASSAIRQFVTDVFWDELDYLVIDMP
                     PGTGDIHLTLMQTVPVTGVIVVTTPQDVALADAKKGIAMFGQAQLKVPIIGLVENMSY
                     FTPAELPDHKYYIFGKEGGKRLAEEYDLPFLGQIPLVQSIREGGDNGVPIMMSEDAIT
                     KKAFLDFAGNAARSIAMLNANITQEKVAEVVA"
     gene            complement(3228..3809)
                     /locus_tag="VKQ52_06435"
     CDS             complement(3228..3809)
                     /locus_tag="VKQ52_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86853.1"
                     /translation="MKQPLTTLVILLLFALNFVSQPACAQAPRQDTLRTGTARASDTL
                     RIHAANAAAGDDDEFNVILLAVLTAFICIVIGATLAGSMAATLVMIGLFGLVSAGVLS
                     AGILVALYRRSITAGFKTILAMVCSFGGILIGVVSFYLINRIFQLHLSGVAVLLIGGF
                     SGLVGGMLLGMAVFLVIRVFLNYCRQKLKLILN"
     gene            3859..4470
                     /locus_tag="VKQ52_06440"
     CDS             3859..4470
                     /locus_tag="VKQ52_06440"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HLZ86854.1"
                     /translation="MYIPRRYEEKDKEKIHAFIRENSFAILISIQEGRPIGTHIPLQL
                     ETGADGKDILMGHISRGNEQKHTLTDGAKVLAIFPGPHAYISPRWYTELKVPTWNYIS
                     VHVYGTVSIIEGEALRAALTRLMDTYEGHQPQPVQIDEIPEKELRADLRGIVGFQITI
                     DEIQAAYKLSQNRDETSYHQVIQHLDEGDETSRAVAAEMKKRK"
     gene            4480..5289
                     /gene="pyrF"
                     /locus_tag="VKQ52_06445"
     CDS             4480..5289
                     /gene="pyrF"
                     /locus_tag="VKQ52_06445"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HLZ86855.1"
                     /translation="MTRQQLIARIRSTGSYLCVGLDTDRTKIPPHLLSHPDPVFEFNK
                     QIIDATKEHCVAYKINTAFYEADGLKGWESMEKTVNHIPATHFRIADAKRGDIGNTSA
                     QYARAFFETLAFDAITVAPYMGEDSVRPFLEYKDKWTILLGLTSNSGAADFELQPAGG
                     GLLYEKVLTTAARWGTPGNLMYVIGATQADEFTRIRSLTPDHFYLVPGVGAQGGSLEA
                     ISKKAMNKDVGLLVNASRAIIFASGGEDFAEAAATAARAYRQEMQGYLPRT"
     gene            complement(5243..>6108)
                     /locus_tag="VKQ52_06450"
     CDS             complement(5243..>6108)
                     /locus_tag="VKQ52_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HLZ86856.1"
                     /translation="IRQGEKGFNWWEFFRTNLTLQVLVAAIIAGIVFSMQARRERIMR
                     VLGVCTKFVFRVLRYVMLLAPLGAFGGMAYTIGKYGIRTLIPLGKLMLTVYGTMILFV
                     FVVLGLILRYFKLNVWTYLKNIKEEILIVLGTSSSEAALPSLMAKLETMGCSQPVVGL
                     VVPTGYSFNLDGTSIYLSMSVIFLAQLYNVSLSGGELLTILGILMVTSKGAAGVTGSG
                     FIVLASTLTAIQKIPVEGLAFLLAVDKFMSEARAITNIIGNGVATLVIAKSEKEFRSV
                     EGSPAFPAGTL"
BASE COUNT         1489 a         1859 c         1562 g         1198 t
ORIGIN      
        1 aatgcagccg cgcagataac ggccggcgac atcggcgcct cccagcaggg gaatgtcgtc
       61 accgaggata acgtacaacc cggaaccctc aacgtgggac aactatccgc ctgcggtgtc
      121 accatcaatc aatacgttac cgtcaccttc aataacagca cctatagcca gacttccggc
      181 agttatttgt attacggttc ggggaatttc gaatcctttt cgaatcccta tatgaccttc
      241 tccgtcccgg gcctctccgg aacagggacc ttcagccctt ccaacttcat gctgtataca
      301 ggaaactatc gcatcgccgc cgccacggac aatacgctca gggtaaacgt caccaccttc
      361 ggcgcggtga accagtacgt catagggacc ctcggcggga atgtatatga ttctaccagt
      421 aaaacggtat acccgcttac cggaaacttc aaactccttc gtaccaacta agacggggaa
      481 aggttcagga agcaaacctc gcactgacga ccagctcctt gctgcccgcg gtgtatttat
      541 aaaacccttc gccgctcttg atgccaaggt aaccggccgt gaccatattg gtcagcaggg
      601 gacagggcgc atatttcggc tggccaaacc catcgtgcag cacgcggagg atcgagaggc
      661 acacatccag cccgataaaa tccgccagtt gcagcggccc catcgggtgg gccatgccaa
      721 gcttcatgat cgtatcgatc tcattcaccc ccgcaacgcc ttcataaagg ctatagatgg
      781 cctcattgat catcggcatc aggatacggt tggcgataaa cccgggatag tccttgacca
      841 cacagggcgt cttccccagc ttctccgcga gcgtcaccat ctggcgggtt accgatgcat
      901 cggtcgcata cccgttgatg acctcgatca gcttcatcac cggaacgggg ttcatgaaat
      961 gaagtccgat cacggccgcc ggccggcgcg tcacggaggc gatcttactg atggagatgg
     1021 aagaggtatt ggtggccagg atacagccca tgggcgcatg ttcatccagt tgccggaaga
     1081 tattcagctt taattcggta ttctccgttg ccgcttccac caccaggtcg gcttttggca
     1141 cacccgccgg gatcccggta tgggtagtga tggcctgcag cgtcgacttc ttttcctcct
     1201 ccgacaataa tcccttggct atttgccggt ccaggttgag cccgatcatc ttcagcgcct
     1261 tttccagctg atgcgccgaa gtgtcgacca ggctgacggt aaatcctttc tgtgcgaaaa
     1321 catgcgcgat gccattaccc atggtaccgg cgccgatcac ggagatattt tgcatatggc
     1381 ggctaattga ctgctaattg ttatttcgga aatcaccccg cttccaggcc tggataaaat
     1441 cagcccaggc aagaggattg aagatgttct ggggaggcgc ctgcccattg tagtagtagc
     1501 gctgggcatt ttgtcgcaga taggtgctgg ccgcctcgcc cgaattggca ggcagactcc
     1561 tggacagtat ccgccgcttc gcggcatcgt tgttctgacg ggctatttca atatcgtcat
     1621 ccggaacctt cgtattcaca aaatcccgct cgaattgctc ccgcgtgggc cgcgccgcga
     1681 tgatcgtggc gggcagatat tgcgcatcca ccaccatcag ctggatcaga ctgatctggt
     1741 tgccgcgaac agtatcctga atactgtaca gaacgggttt ataacccacg ctagtgaact
     1801 cgatctggtc tcccttgagc acaacgatcg aaaagacacc ctgatcgttg gaaaaagtac
     1861 cctggttccg tccctttacc acaatactca cagagggtat cgctttcaag ctgtcggctg
     1921 tcattatgac accataaagt tgtactacag aatcttttag cttatcgaat tgggcatgga
     1981 cggcaaaggg ggcggaaaga agcagaaagt atagtacaaa ttttttcatt cagctcaaaa
     2041 ttaaactttt aaagtttcaa ttagcaaaca aagtaaagct gccagtggtt aattttgtct
     2101 caaattagcc tttttaacaa aatcttcgaa atgaccccgg aaaaagtttt agcggccctt
     2161 ggtaacgttc aggaacccga tctggggaaa gatatcgtga ccctgaatat ggtgaaggat
     2221 ctcgagataa acggcaacta tgtcgctttc accgtcgttc tcaccacgcc cgcctgtccg
     2281 atgaaggacc atatcaaaaa tgcctgtatc aacgccataa aactgctggt aaataaagag
     2341 gccaccgtca acgtgcgctt taccgccaac accaccacgc aacgcaaaga tgcgcgcgaa
     2401 atgctcccga aggtgaagaa catcatcgcg gttgtcagcg gcaagggtgg ggtaggaaag
     2461 agcaccgtcg ccgccaactt cgccatcgca ctggcccgcg gcggcgccag cgtcggcctg
     2521 atggacgccg atatctacgg ccccagcgtg cccatcatgt ttggcgtcag aggacaacgc
     2581 ccgatgatga tggacggcgg acagaatggc aaaggcctga tcgtcccgct cgaaaaatac
     2641 ggcatcaaac tgatgagcat cgggttgctc gtcgatgaga agaatgccgt ggtctggcgc
     2701 ggcccgatgg ccagcagcgc tatccggcaa ttcgtaaccg atgtgttctg ggacgaactg
     2761 gattatctcg tcatcgatat gccgccgggg acgggagaca tccatctcac ccttatgcag
     2821 acggttcccg tgacaggcgt catcgtcgtc actacccccc aggatgtagc gctcgccgat
     2881 gcgaaaaaag gcatcgccat gttcggccag gcgcaactaa aggttcccat catcgggctc
     2941 gtggagaata tgagttactt tacccccgcc gaactccccg atcacaaata ctacatcttc
     3001 ggtaaggaag gcggcaaacg gctcgccgaa gaatatgacc tccccttcct ggggcagatc
     3061 cccctcgtac aaagcatccg cgagggcggt gacaacggcg ttcccatcat gatgagcgag
     3121 gacgccatca ccaaaaaagc attcctcgat tttgccggca acgcagcccg cagcatcgcc
     3181 atgctgaatg caaacataac acaggagaaa gtagccgagg tcgtagccta gttcaggatc
     3241 aatttcagct tctgccggca gtagttgagg aacacccgga tgaccaggaa caccgccatg
     3301 cccagcagca tgccgccgac aagaccgctg aagccaccga tcagcaacac ggcgaccccg
     3361 ctgagatgca gttggaagat gcggttgata agatagaagc tgaccacgcc gataagaatg
     3421 ccgccaaagc tgcagaccat cgccagtatg gtcttgaaac cggccgtgat cgatctccgg
     3481 tagagcgcca ccaggatccc cgcggacagg acacccgcgg ataccagccc gaaaagcccg
     3541 atcatcacca gcgtcgcagc catcgaaccg gcaagggtgg cgccgatgac gatacagata
     3601 aaggcggtca gcaccgccaa caggatcacg ttgaattcgt catcgtcccc ggccgcggca
     3661 tttgccgcat ggatcctcag cgtatcgctg gccctggccg tgccggtgcg gagcgtatcc
     3721 tgccgggggg cctgggcaca ggcaggctgg ctgacaaagt tcagcgcaaa caggaggaga
     3781 atcacgaggg tggtcagtgg ttgtttcata gatgagccag gtgtacccta aaaatacact
     3841 ttttccgtaa tttgcggtat gtacatcccc cgccgctacg aagaaaaaga taaggagaag
     3901 atccacgcct ttatccgcga gaattccttt gcgatcctca tttccattca ggaaggccgg
     3961 cccatcggca cccatatccc gctgcaactc gagacgggtg cggatgggaa agacatcctc
     4021 atgggccata tctcccgcgg caatgaacaa aaacacaccc tcaccgacgg cgcgaaagtg
     4081 ctcgccatct tccccggtcc ccatgcctat atctccccac ggtggtacac cgaactaaag
     4141 gtgcccacct ggaactatat ctccgttcat gtctacggga cggtgtccat catcgaaggc
     4201 gaagccctcc gcgccgccct cacccggctc atggacacct acgaagggca tcagccccaa
     4261 ccggtgcaga tcgacgagat ccccgaaaag gaactccggg ccgacctccg cggcatcgtc
     4321 ggttttcaga tcaccatcga cgagatccag gccgcttata agctcagcca gaaccgcgac
     4381 gaaacgagtt atcaccaggt gatccaacac ctcgatgaag gcgatgagac ctcccgcgcc
     4441 gtcgccgccg aaatgaaaaa aagaaagtaa ttttaccgca tgacccgtca gcagttaatc
     4501 gcccgcatcc gtagcaccgg ctcctatctc tgcgtggggc tggatacgga caggacaaag
     4561 atcccccctc atctcctttc gcatcccgac cccgtgttcg aattcaataa acagatcatc
     4621 gacgcgacaa aagaacattg tgtcgcctac aagatcaata cggccttcta tgaagcggat
     4681 ggattgaaag gctgggagtc gatggaaaag acggtcaacc atatccccgc gacgcatttc
     4741 aggatcgccg acgccaaacg cggcgatatc ggcaacacct ccgcccaata cgcccgcgcc
     4801 tttttcgaga ccctggcctt cgacgccatc accgtggcgc cctatatggg ggaagacagc
     4861 gtccgcccct tcctcgaata caaggacaaa tggaccatcc tcctggggct cacttccaac
     4921 agcggcgcag cggatttcga gctgcaaccc gcaggcgggg gcctcctgta cgaaaaggta
     4981 ctgaccacgg ccgcccgctg gggtacaccc ggcaacctca tgtacgtcat cggcgcaaca
     5041 caggccgacg aatttacccg catccgaagc cttacccccg atcatttcta tctcgttccg
     5101 ggcgtcggcg cccagggagg cagcctcgaa gccatctcca aaaaagcgat gaataaagac
     5161 gtgggactgc tggtcaacgc cagccgtgcg atcatcttcg cctccggcgg cgaagacttc
     5221 gcggaggccg ccgcaacagc ggctagagcg taccggcagg aaatgcaggg ctaccttcca
     5281 cggacctgaa ctccttctca ctctttgcga tcaccagcgt cgccaccccg ttgccgataa
     5341 tattggtgat ggccctggcc tcgctcatga acttgtcaac ggccagcagg aacgccagcc
     5401 cctcgacagg gatcttctgg atggccgtca gcgtcgaggc gaggacgata aaaccactac
     5461 ccgtaacacc cgccgccccc ttcgatgtca ccatgaggat acccaggatg gtcagcaact
     5521 ccccgccgct gagcgacacg ttgtacaatt gcgccaggaa gatcacggac atcgaaagat
     5581 agatcgaggt tccatcgaga ttgaaggaat aacccgtcgg caccaccagc cccaccaccg
     5641 gctgactgca acccatggtt tccagtttgg ccatcagcga aggcagcgcc gcctcggagg
     5701 acgaggtgcc cagtacgatc aggatctcct ccttgatatt tttcaggtag gtccagacat
     5761 tcagcttgaa ataccgcagg atcagcccaa gcaccacgaa cacaaaaagg atcatcgtcc
     5821 cgtataccgt cagcattagc ttccccaacg ggatcagcgt ccggatacca tatttcccga
     5881 tggtataggc cattccgccg aaggcgccca acggcgccag cagcatcaca tacctcagca
     5941 cgcggaacac gaatttcgta cagaccccca acaccctcat gatcctttcg cgccgggcct
     6001 gcatgctgaa cacgatgccc gcgatgatcg ccgccaccag cacctgcagc gtgagattgg
     6061 tgcggaagaa ttcccaccag ttaaaaccct tctccccctg gcggatat
//
LOCUS       DATGFG010000340         4151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_166392, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000340 DATGFG010000000
VERSION     DATGFG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166392"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..604)
                     /locus_tag="VKQ52_06455"
     CDS             complement(8..604)
                     /locus_tag="VKQ52_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86857.1"
                     /translation="MKEMILLLCGIVIGRQLMAQTFSEWFRQNHTQLKYLAELIAALR
                     SYDGLLQKGYATARDGLGDIWDIGEEDLDLHSEHFAELDVASPGVQADATVETIQQYC
                     AWLPAIAEKIEAIWAGQSSQPMYFPEWGLSISGGLRYAAEISRRSLADILAAGRYQMD
                     DAERMRILSLMSLALKNLYGKAVLLLTELDWMTINHSI"
     gene            complement(601..1260)
                     /locus_tag="VKQ52_06460"
     CDS             complement(601..1260)
                     /locus_tag="VKQ52_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86858.1"
                     /translation="MKTRWMVTVVLLLAGQQTFAQDIGSIISAGIKTVIRAVDLSIQR
                     VQTQTIVLQEAQKVVENAMSALELDDIRDWVERQKDLYGEYFQELGEVKSAISAYHRV
                     KEAIQRQEALVAAYQQGLGRFRRDGHFSAAELTQMETVFAGILAESEKNLEQLGKAVG
                     MLVFQMTDQERLAMVDAAADGIDRNYRDLSEFTQQSELLSLQRARDANDYSLLLKLYG
                     L"
     gene            complement(1257..3728)
                     /locus_tag="VKQ52_06465"
     CDS             complement(1257..3728)
                     /locus_tag="VKQ52_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032991 - protein-containing complex
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraG family conjugative transposon ATPase"
                     /protein_id="HLZ86859.1"
                     /translation="MEKNLRDLLPIYGIEREGILSKQGDYTIGFRLRKPEIFTLAADQ
                     YESLHQAYVKGMRVLPPHTILHLQDWFLTDRYPADFTRDEQSFLARSSERFFHERPWL
                     RQESYLFLTRKPKDRQESSSAFSSLLRGRLLPAEATDPRMMTEFREPVSQFVRILEDS
                     GLLTIEPLTPEDLRSGPGRAGLIERYCTLSAEDTPVLRDIEWTPEMRIGDRHCMLFTL
                     ADAEDLPGLCGSRIDYGPYCTDRTRYSIGYASALGPLLPCDHIYNQYLFIDEAGATLK
                     KLESKRLRLQSLAAYSRENAVSEEATNAYLNEALEEQRLPIRAHFNVFAWTENPAEKA
                     GIRNRIGSVIAQLEASPRLETAGAPQIWFAGLPGNEGDFPMNETFRTFAEQAACFLIP
                     EENYRSSRSPFGIRLGDRLTGRPVHVDIDDEPRREGWIGNGNCFVCSGSGGGKSFFMN
                     HLVREYYERGTHVVLIDVGHSYEIQCALHGGYYFTYREDDPIRFNPFFIAEGDELDTE
                     KRESIKTLLLALWKKSSEGHNRAEYVALSSALQGYFEKIARDASVFPCFDSFYEYLKE
                     EFAALLAAEQVKDKDFDLANFLYVLRPFYKGGEFDYLLNARANLDLLRERFIVFELDN
                     IKNHPILFPVVTLIIMELFVGKMRKLKGIRKMIVIEEAWKAIAQEGMAEYIKYLFKTA
                     RKFFAKAVVVTQEVEDIVSSEIIRQAIVNNSDIKILLDQSKFQNKFDEIQALLGITDK
                     QKAEILSINKGREPGRIYKDLWIGLGALHSKVYRLEVSEEEYYVYTSEQKDKVLVGRY
                     IERYGDVKTAITRLVADLRAKKTGL"
     gene            complement(3782..4090)
                     /locus_tag="VKQ52_06470"
     CDS             complement(3782..4090)
                     /locus_tag="VKQ52_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4133 domain-containing protein"
                     /protein_id="HLZ86860.1"
                     /translation="MKTEVYTINKGVNRAIVFRGLQAQYIGWLAGGVITLLLLFAGLY
                     IAGVNPFACLAIIGSAGTVLFRQVYRLSRKYGRWGWMKKTAARRLPGRITVGTRRIFL
                     "
BASE COUNT          825 a         1325 c         1046 g          955 t
ORIGIN      
        1 cgctttttca tatcgaatga tttatagtca tccagtccaa ctccgtcagc agcaggacgg
       61 ccttgccgta aaggttcttc agcgccaggc tcatcaggct caatatccgc atccgttccg
      121 cgtcatccat ttgataccgg ccggctgcaa ggatatcggc cagggagcgg cgggagatct
      181 ccgcggcata ccgcaggccc ccggaaatgg atagtcccca ttccgggaaa tacatgggct
      241 gactggattg tcccgcccaa atggcctcga tcttttccgc aatcgccggc agccatgcgc
      301 aatactgctg tatcgtctcc acggtcgcat ccgcctgtac accgggactc gccacatcca
      361 gttcggcgaa atgttccgaa tgcaggtcca ggtcttcctc cccgatatcc cagatatctc
      421 ccagcccgtc ccgcgcagtg gcataccctt tttgcagcag gccgtcatag cttcgcagcg
      481 cggcgatcag ttccgccagg tatttcagct gcgtgtggtt ctgccggaac cattccgaaa
      541 aggtctgcgc catcagctgc cgtccgatca caatgccgca cagcagcagg atcatctctt
      601 tcatagtcca tacaatttta gaagtaaaga ataatcgttt gcatctctcg cccgttgcag
      661 gctgagcagc tcgctctgct gcgtgaattc cgacaggtca cggtaattcc gatcgatccc
      721 gtctgccgcg gcatccacca tcgccagtcg ctcctggtcc gtcatctgga agaccagcat
      781 gccgactgct ttgcccagct gttcgagatt cttttcgctt tccgcaagaa tacccgcaaa
      841 cacggtctcc atctgtgtca gttccgcggc gctgaaatgc ccgtcccttc gaaaccgacc
      901 cagcccctgc tgataggcgg caaccagcgc ctcctgtcgc tggatcgctt ccttcacccg
      961 gtgataggcc gagatcgccg acttcacctc gcccagctcc tggaaatatt cgccatacag
     1021 gtccttctgc cgctccaccc aatcccggat atcgtccagc tccagcgcac tcatagcgtt
     1081 ctccaccacc ttctgcgcct cctgcaggac gatcgtttgg gtctgcaccc gctgaatgct
     1141 caggtctact gcccggatca cggtcttgat ccccgcgctg atgatcgagc cgatatcctg
     1201 cgcaaacgtc tgctgtcctg ccagcaacaa aaccaccgtc accatccacc ttgttttcat
     1261 aatcccgttt tttttgcccg cagatcggcc accagccgcg tgatcgccgt tttgacgtcc
     1321 ccataccgct cgatataccg accgaccagc accttgtcct tctgctcgga cgtatagaca
     1381 tagtactctt cctccgacac ctcgaggcga tacactttgg aatgaagggc gccgaggccg
     1441 atccacaggt ccttgtagat ccgccccggc tcgcgcccct tgttgatgct gaggatctcg
     1501 gccttctgtt tgtccgtgat gccaagcagc gcctgtatct cgtcgaattt gttctggaat
     1561 ttgctctgat cgagcagtat cttgatgtcc gagttattga caatggcctg acggatgatc
     1621 tccgatgaca cgatatcctc cacttcctgg gtcacgacca cggctttggc gaagaacttt
     1681 cgtgccgtct tgaacaggta tttaatgtac tccgccattc cctcctgggc gatcgccttc
     1741 catgcctctt ctatgacgat catcttgcgg atacccttca actttcgcat ctttccgaca
     1801 aagagctcca tgatgatgag ggtcaccaca gggaagagga tgggatggtt tttgatattg
     1861 tccaactcga atacaatgaa cctttcccgc agcaggtcga ggttggcccg cgcgtttagc
     1921 aggtagtcga attctccgcc tttgtagaaa ggccgcagca catagagaaa attagccagg
     1981 tcgaaatcct tgtccttcac ctgctcggct gcgagcaagg cggcaaattc ctccttcagg
     2041 tactcataga agctgtcgaa gcaggggaat actgaggcat cccgcgcgat cttctcgaaa
     2101 tacccttgca acgcgctcga caaggctaca tattccgccc ggttatgccc ctccgatgac
     2161 ttcttccaca atgccagcag cagcgtctta atgctttccc gcttttctgt gtccagttca
     2221 tccccctctg caataaagaa cgggttgaat cggatgggat cgtcctcacg ataggtgaaa
     2281 tagtagcctc catgcagtgc gcattgtatc tcgtagctat gtccgacatc tatgagcaca
     2341 acatgcgtcc cccgctcata atactcccgt accaggtggt tcatgaagaa gctcttgcct
     2401 ccgcccgagc ctgaacacac gaaacaattc ccgtttccaa tccatccttc cctgcgcggt
     2461 tcgtcgtcga tatcgacatg caccggccgg cccgtcagcc gatcccccag ccggatgccg
     2521 aacgggctcc tgctgctgcg gtaattctct tcggggataa gaaagcaggc cgcctgttcg
     2581 gcaaacgtcc ggaaggtttc attcatcgga aaatcgcctt cgttacccgg cagcccggca
     2641 aaccagatct gcggcgctcc cgcggtctcc agccgtggac tggcttccag ctgagcgatc
     2701 acgctcccga tccggttgcg gatacccgcc ttctccgccg ggttctccgt ccaggcgaag
     2761 acattgaagt gcgcccgtat gggcaggcgc tgctcttcca gcgcctcgtt caggtaggca
     2821 ttggtggctt cctcgctgac ggcattctcc cgggaatagg cggccagcga ctggagccga
     2881 agccgtttgg actccagctt cttcagcgtg gcgccggcct catcgataaa gagatactga
     2941 ttgtagatat ggtcgcaggg taggagagga cccagcgcgc tggcataccc gatagaatac
     3001 cgggtgcggt cggtgcagta gggtccgtag tcgatccggc ttccacagag acccggcagg
     3061 tcttcggcat ctgccagcgt gaagagcata caatgccggt cgccgatccg catctccggt
     3121 gtccattcga tatcccgcag caccggcgtg tcttccgcgc taagcgtgca ataccgttcg
     3181 atcagccctg cccgtccggg cccactgcgc aggtcctccg gcgtgagggg ctctatagta
     3241 aggagaccgc tgtcttcgag gatgcgcaca aactgcgata cgggctcccg gaattccgtc
     3301 atcatccgcg ggtcggtcgc ctcggcaggc agcagcctgc cccgcagcag gctcgaaaag
     3361 gcggagctcg attcctgtct gtcctttggc ttccgcgtca ggaacaggta cgactcctgc
     3421 cgcagccagg gccgttcgtg gaagaagcgt tcgctgctcc gggcgagaaa gctctgttcg
     3481 tctcgcgtga agtcggcggg gtaccggtcc gtcaggaacc agtcctgcag gtgcaggatg
     3541 gtatggggcg gcaatactcg catccccttg acgtatgcct ggtgcagcga ttcatattga
     3601 tccgccgcca gagtaaagat ctccggcttc ctaagccgga aaccgatggt ataatctccc
     3661 tgtttgctca ggatgccctc ccgttcgatc ccatagatgg gcagcaggtc acgtaggttt
     3721 ttttccattt caacagcatt tgacacttca cagtatttga cacttcacaa aatgtaactc
     3781 ctcacaaaaa gatccttctc gtcccgacgg ttatccgccc cggcagcctt cgcgccgccg
     3841 tcttcttcat ccatccccat cggccatact ttcggctcag ccggtacacc tgtcggaaga
     3901 gcaccgttcc cgcactcccg atgatcgcca gacaggcgaa gggattcacc cccgcgatgt
     3961 acagtccggc gaacaacagc agcaaggtga tgacaccacc cgccagccag ccgatatact
     4021 gcgcctgcaa tccgcgaaac acgatagcgc ggttgacgcc tttgttgatg gtatatactt
     4081 ccgttttcat ccgcacgtaa cgtcttgtca atggccggat ggaatctcgc cattaatgaa
     4141 tattgctcga t
//
LOCUS       DATGFG010000341         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1120192, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000341 DATGFG010000000
VERSION     DATGFG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120192"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2203
                     /locus_tag="VKQ52_06475"
     CDS             167..2203
                     /locus_tag="VKQ52_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86861.1"
                     /translation="MKRSTCLRFLLLLLLASPLFARAQDDWPRSIIAADGSIIRISYP
                     QPDSFIDNNIQFRAAFCLTRKGAHQPVYGSFQAWSVFETDRNNRTLSLLAANLTRLHL
                     VQPLSPDTLADLRETIECGLSAIGSDLSLDRLISLLDYVPAPRDSPTALAHTPPRIIL
                     WGRPAILVLIDGIPRFKWNKDYGLYAVANSPNTIMESSDGWFYLYGGRHWYLAPTPEG
                     PFQHTTYIAPDLHHVEALINSINDREAEHSDTLREGNEGVIDVLFSTTPATLVCTKGA
                     PQFAPIRGTHLLYAANSDDDLFLDSLRHTYYLLVSGRWFSSPGLSGQWKYVPQDSLPR
                     DFAAIPEGSAADRVLACVAGTDAAREAIIDAGIPQTITIDRRTASTRVIYDGAPKFAP
                     IHGTHLQYAYNTPSIVLRSGNSYYCVDKGIWFGSQNPGGPWTACTSRPEEVSLIPPDC
                     PVYTCKYVYIYGAGGNAVTTGYTTGYLGSYVDGPTLVYGTGYHYPCYAGNIVFPYSWT
                     YGFNMWYNPWFGWSLGYDCSPDWLNTSMAWGEGAWKGGWFGPSDYRPPYIWHHFSGHG
                     LYERDYRLIAASSYNNNLYTLRNDLPARPGPEFTVTDGDGNLYCPDGRNGWLQRKEQS
                     WVPLDTAHRTTLTYLDRLRHRLLRGEMRAHNFRQASGVYIVSSPQSPDHGKTTN"
     gene            2184..2897
                     /locus_tag="VKQ52_06480"
     CDS             2184..2897
                     /locus_tag="VKQ52_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86862.1"
                     /translation="MAKQPTNGQKTPKKKNRWLYTLGWIAAPFFLFLIFYFSGILRGS
                     PQQISWDEFEKQILARHAADSMDIVNNEDAEVFIKKSFADDPYFKPVMKPGTEKMIEP
                     GPHYTFHIGSVVTLQQKIDEAQKDIPPAQRIRLNYVKKGGIFSGLLGWLIPLVAFFFF
                     MRLLSWLPRPLVSPAPISPISATKPPCSPPAEKARGWKNRIFSMPSTASSPVSRKKAA
                     SSPLQRSSSSLIMKPVTPS"
BASE COUNT          609 a         1061 c          687 g          560 t
ORIGIN      
        1 atcatgaact tccttatccg tcgactccac cgcctccggc tcatccgggc ggccggtccg
       61 ggcgggacaa tatcatctcc ccgcctgacc accgtcactg acaggcctcc gccgcaacag
      121 tagcttcaca tcggtacacc cattcactat ttaaagcaat ggcatcatga aaagaagcac
      181 ctgcctgcgg ttcctgctcc tccttctcct ggcctcgccc ctcttcgccc gcgcccagga
      241 cgactggcca cgctccatca tcgccgccga cggcagcatc atccgcatat cctatcccca
      301 gcccgactcc ttcatcgaca acaacatcca attcagggcc gccttttgcc tgacaagaaa
      361 aggcgcccac caacccgtat acggttcctt ccaggcatgg tcggtcttcg aaacggacag
      421 gaacaaccgg acactgagcc tcctggccgc caacctgaca aggctgcacc tggtgcagcc
      481 tctctccccc gatacgctcg ccgatctccg cgaaaccatc gaatgcggcc tgtcggccat
      541 cggcagcgat ctgtccctcg accggctgat ctctttgctc gattatgtgc ccgcgccccg
      601 cgactccccg actgccctcg cgcatacccc accccggatc atcctctggg gccgcccggc
      661 catcctcgtc ctgatcgacg gcatcccccg cttcaaatgg aacaaagact atggcctcta
      721 cgccgtcgcc aacagcccca atacgatcat ggaatccagc gacggctggt tctacctgta
      781 tggcggccgc cactggtatc tcgccccgac ccccgaaggc ccctttcagc ataccaccta
      841 tatcgctccc gatctccacc acgtcgaagc cctgatcaac agtatcaacg accgggaagc
      901 cgaacactcc gatacgctcc gggaaggcaa tgaaggcgtg atcgacgtcc tcttcagcac
      961 aacccccgcg acactggtct gcacaaaggg ggcgccccaa ttcgctccca tccgtggtac
     1021 ccacctgctc tacgcagcca acagcgacga cgatctcttt ctcgacagcc tccgccacac
     1081 ctattacctg cttgtctccg gccgctggtt cagctcaccc ggcctttcgg gccagtggaa
     1141 atatgtgcca caggattcgc ttccgcggga tttcgccgcc atccccgaag gctcggccgc
     1201 cgaccgcgtc cttgcctgcg tggcaggtac cgacgccgcc cgggaggcca tcatcgacgc
     1261 aggcattccc caaaccataa ccatcgatcg ccgcaccgcc tccacgcggg tcatctatga
     1321 cggggccccg aaattcgccc ccatccacgg cactcatctc cagtatgcct ataatacacc
     1381 ttcgatcgta ctgcgctccg gcaatagcta ttattgcgtg gacaagggca tctggttcgg
     1441 ttcgcagaac ccgggcggcc cctggacggc ctgcacgagc cggcccgaag aggtaagcct
     1501 catccccccc gactgcccgg tctacacttg taaatatgtc tatatctacg gcgccggcgg
     1561 aaatgccgtc accaccggct acaccaccgg ctacctgggt tcctatgtcg acgggcctac
     1621 acttgtgtac ggcaccggct atcattaccc ctgctatgcc ggcaatatcg ttttccccta
     1681 ctcctggacc tatgggttca atatgtggta caatccctgg ttcggctgga gccttggcta
     1741 cgactgcagc cccgactggc taaacacatc catggcctgg ggcgaaggcg cctggaaggg
     1801 cggctggttc ggcccctccg attatcgccc gccctatatc tggcaccact tcagcggcca
     1861 cggcctctat gaacgggact accggctcat cgccgccagc agctataaca ataatctata
     1921 cacgctaagg aacgacctgc ccgcccgccc cggtcccgaa ttcaccgtca ccgacgggga
     1981 cggtaacctg tactgccccg acggccggaa cggctggctg cagcgtaaag aacaatcctg
     2041 ggtcccgctg gacaccgccc accgaacgac cctgacctat ctcgaccgcc tgcgccaccg
     2101 gcttctgcgc ggcgaaatgc gcgcccacaa tttccgccag gccagcggcg tctatatcgt
     2161 ctcatcccca caatccccgg atcatggcaa aacaaccaac taacggccaa aagacaccga
     2221 aaaaaaagaa tcgctggctc tataccctgg gctggatcgc cgcacccttc tttctcttcc
     2281 tgatcttcta tttctccggt atcctgcgcg gcagtccgca acagatcagt tgggatgagt
     2341 tcgaaaaaca gatcctcgcc cgtcatgccg ccgacagcat ggatattgtc aataacgaag
     2401 acgccgaagt attcataaag aaaagctttg ccgacgatcc ttatttcaaa cccgtcatga
     2461 aacccggcac ggaaaagatg atcgaaccgg ggccccacta cacctttcac atcgggtcgg
     2521 tggtaaccct gcagcaaaag atcgacgagg cccaaaaaga catcccgccg gcccagcgca
     2581 tccgcctcaa ttatgtaaag aaaggcggca tcttctccgg cctcctgggc tggctcattc
     2641 ccctggtcgc ctttttcttc ttcatgcgcc tcctctcctg gctgcccaga cccctggttt
     2701 ctccggcgcc gatatcgcca atatctgcaa cgaagccgcc ctgttcgccg cccgcagaaa
     2761 aagcgcgggg gtggaaaaac aggattttct cgatgccatc gaccgcatcg tcgccggtct
     2821 cgagaaaaaa agccgcatca tctcccctgc agagaagcag ctcatcgctt atcatgaagc
     2881 cggtcacgcc gtcgtaagct ggttcctgcc acaggta
//
LOCUS       DATGFG010000342         5785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_420939, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000342 DATGFG010000000
VERSION     DATGFG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420939"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VKQ52_06485"
     CDS             <1..430
                     /locus_tag="VKQ52_06485"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86863.1"
                     /translation="LREKLGATHPGSPAVNSNPTPPAANSTPHPDSAANLQILTGDFF
                     NHQGQYDLIIEQTFFCSLEPAQRPAYVDKTHHLLRPGGRLAGLLFDRNFPAGPPFGGN
                     KAEYQKLLEKKFTLKTLAPCYNSIKPRAGTELFFIAVRPD"
     gene            complement(433..1470)
                     /locus_tag="VKQ52_06490"
     CDS             complement(433..1470)
                     /locus_tag="VKQ52_06490"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HLZ86864.1"
                     /translation="MTPKQAGYYFPAEFSRHSATWLSWPHKEASWPGKIASIYPFYSQ
                     FVKELAKGEEVCINVADEAMKAFAIRHLEAAGADLEMVRFFLHPTNDAWCRDHGPAFL
                     INPAAAQKKVIVDWNYNAWGDKYPPYDLDDVIPTLVGKALNLPVYHPGIVMEGGSVEF
                     NGKGTLLTSTCCLLNVNRNPGLGRDRIEQYLRDFYGVEQILWVKEGIVGDDTDGHIDD
                     TVRFVDEDTVVTVIEEDKQDENYALLQENLADLSKMRLLNGKQLNIVELPMPEAVIYE
                     DQRLPASYANFYIANESVIVPTYRCDRDEKALRILQECFPARRVIGIDSTEIIWGLGS
                     FHCLSQQEPAV"
     gene            complement(1516..2391)
                     /locus_tag="VKQ52_06495"
     CDS             complement(1516..2391)
                     /locus_tag="VKQ52_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HLZ86865.1"
                     /translation="MSNVKVGLVQMSCTGDKAANLRKAMDKIREAAAKGAQIVCLQEL
                     FTSLYFCDVEDYENFKLAEPIPGPSSEALGRLAGELGVVIIASLFEKRAQGLYHNTTA
                     VLDADGSYLGKYRKMHIPDDPAYYEKFYFTPGDLGYKVFKTKFATIGVLICWDQWYPE
                     AARITALMGAEILFYPTAIGWATSQDEATNTEQYNAWQTIQRSHAVANGVHVVSVNRV
                     GFEQDGVMKFWGGSFVANPFGSLLYKASHEQEETTVVELDTQATDRYRTHWPFMRDRR
                     IDSYQPITKRYIDED"
     gene            complement(2468..3535)
                     /locus_tag="VKQ52_06500"
     CDS             complement(2468..3535)
                     /locus_tag="VKQ52_06500"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HLZ86866.1"
                     /translation="MSLPKFSHTTQSFHTELKKRISNYFEEVGKSQTGNYNLFIKAVI
                     LMVSFVFVYIHLVFFTPGVLWAILECVLLGCIVAAIGFNVMHDGAHGSFSKYKWVNHF
                     AAFSLNILGGNSFMWNQKHNVIHHAFTNVDGVDDDIDIQPWMRMSETQKKYKLHKYQH
                     LYFWFLYSLLYIFWIFVLDYQKYFKSKVGAMPLKRMGLADHFVFWGFKLFHAFLFVAL
                     PIYVIGFSSWVIGFVIFTCVAGFVLSLVFQLAHTVEHTAFPKADVVTGKLEDEWAVHQ
                     IKTTANFATNNPVVSWLVGGLNFQIEHHLFPKISHVHYPAISKIIRQACQEYGLVYIE
                     YPRVRYAVASHVAFLRQMGRK"
     gene            complement(3648..4385)
                     /gene="bshB1"
                     /locus_tag="VKQ52_06505"
     CDS             complement(3648..4385)
                     /gene="bshB1"
                     /locus_tag="VKQ52_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HLZ86867.1"
                     /translation="MKLDILAIAVHPDDVELGCAGTLLMEKRNGKKTGVVDLTRGELG
                     TRGTAELRAEEAVKAAAILGLDARENLGMADGFFRNDEEHQRRLIRAIRKYRPEIVLG
                     NSLEDRHPDHGRAGHLISDSCFLSGLRKIGTLDDEGRPQEHWRPKYVFHYIQDRYAAP
                     SFVYDITPVFETKLASIRAYSSQFFSAEYGENEPQTYISTPEFLNAIIGRHQMFGKMI
                     GVPYAEGFITEKMIGVRDFDSFVQMDT"
     gene            4515..>5785
                     /gene="lpdA"
                     /locus_tag="VKQ52_06510"
     CDS             4515..>5785
                     /gene="lpdA"
                     /locus_tag="VKQ52_06510"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HLZ86868.1"
                     /translation="MAYDLVVLGSGTGGYPAAIRAAQLGFKVAIIEKENLGGVCLNWG
                     CIPTKALLKSAQVYQDILHSKEFGIDASGTVDFPAVIKRSRGVADKMSKGVQFLMRKN
                     KIDVVLGYGKLKAKGQVEVTGADGKTQIVEGKHIIVATGARSRELPNLKQDGQKVIGY
                     REAMTPAQKPNSIIIVGSGAIGVEFAYFYYSMGAKVTIVEFLPRIVPVEDEDISKELE
                     KNYKKYGIDILLSSEVTGVDTSGNGVKAKVKTPTGEITLEADVLLSAVGISANIENIG
                     LETLGIKTDKGKIVVDKFYQTSVPGIYAVGDCVPGQALAHVSTKEAIICVENIAWMEK
                     KFAHQPEMLDYNNIPGCTYCTPEIASAGLTEKQARDAGYEVKVGKFPLSASGKATGAG
                     HTEGFVKVVFDAKYGEWLGTHMIGYNVTEIIA"
BASE COUNT         1418 a         1805 c         1354 g         1208 t
ORIGIN      
        1 gctccgcgaa aaactcggcg ccacccatcc gggctccccc gccgtcaact caaaccccac
       61 tccgccggcc gccaactcca ccccccatcc tgactccgcc gccaacctcc aaattctcac
      121 cggcgacttc ttcaaccacc agggacaata cgacctcatt atcgaacaaa ccttcttctg
      181 ctctctcgaa cccgcacaac gcccggccta tgtcgataag acccaccatc tcctccgccc
      241 cggcgggaga ctcgccggcc tcctcttcga ccggaacttc cccgctggcc cgccctttgg
      301 cggcaataaa gccgaatacc aaaaattact ggaaaaaaaa ttcaccctga agaccctggc
      361 cccctgctac aactccataa aaccgcgcgc cggaaccgaa ctcttcttca tcgcggtccg
      421 ccccgactaa cactacaccg ccggctcctg ctggctcagg caatggaaac tccccagccc
      481 ccagatgatc tcggtggaat cgatcccgat cacccgccgc gccgggaaac actcctgcag
      541 gatccgcaac gccttctcat cccggtcaca ccggtacgtc ggcacgatca ccgactcgtt
      601 ggcaatataa aaattggcat aggaagccgg cagccgctga tcctcataga tcaccgcctc
      661 cggcatcggc agctccacga tattgagctg cttgccgttt aacaatctca ttttcgaaag
      721 atccgccaga ttctcctgca gcagggcata attctcatcc tgcttatctt cctcgatcac
      781 cgtgacgacc gtatcctcat ccacaaaccg caccgtgtca tcgatatgcc catccgtatc
      841 gtcaccgacg atcccttcct tcacccacag gatctgctcg acaccataaa agtcacgcag
      901 atactgctct atccggtcac gccccaaacc cgggttgcgg ttaacattca acaaacagca
      961 ggtcgacgtg agcagcgtgc ccttgccgtt gaattccacc gaaccaccct ccatgacaat
     1021 acccggatga taaacaggca ggttcaacgc cttcccaaca agcgttggga tcacatcatc
     1081 gagatcatag ggcggatact tgtcacccca ggcattgtaa ttccagtcca cgatcacttt
     1141 cttctgcgcg gccgccggat tgatcagaaa ggcaggtcca tgatccctgc accaggcatc
     1201 attggttgga tgcagaaaga accgcaccat ctccaggtcg gcgcccgccg cctccaggtg
     1261 ccggatggca aaagccttca tcgcctcatc ggcgacgttg atgcaaacct cctccccctt
     1321 cgccagctct tttacaaact gactgtaaaa aggatagatc gacgcgatct ttccgggcca
     1381 ggaagcctcc ttgtgaggcc agctaagcca ggtagcacta tgtctgctga actctgccgg
     1441 gaaataatag cctgcttgct tgggtgtcat ataagaaagt gtccttgtcg gtctgccgtc
     1501 ctctccaccg ccgccctaat cttcgtctat ataccgtttg gtaatgggct gatacgaatc
     1561 gatccgccgg tcccgcataa acggccagtg cgtacgataa cgatccgtag cctgcgtatc
     1621 gagttccact accgtcgtct cctcctgttc gtgcgaggcc ttgtatagca aactcccgaa
     1681 gggattggcg acaaaacttc caccccagaa cttcatcaca ccgtcctgct cgaacccaac
     1741 ccggttgaca ctgaccacat gcacgccgtt ggccaccgca tgactccgct ggatggtctg
     1801 ccaggcatta tactgctcgg tattggtcgc ttcatcctgg ctcgtcgccc agccgatggc
     1861 cgtcgggtag aaaaggatct ccgctcccat cagggccgtg atccgcgccg cctcgggata
     1921 ccactggtcc caacagatca ggacgccgat ggtggcgaat ttcgtcttga acaccttata
     1981 ccccaggtcg cccggggtaa aatagaattt ctcgtaatag gccgggtcgt cggggatatg
     2041 catcttccgg tatttgccaa ggtagctgcc gtcggcatcc agcaccgccg tagtattatg
     2101 gtatagcccc tgtgcccgct tctcgaacag cgaagcaatg atcacgacgc caagttcgcc
     2161 cgccagccgg ccaagggctt cggaagaagg ccccggtatg ggctcggcca acttgaaatt
     2221 ctcgtaatct tccacgtcac agaaatacag ggaggtaaac aactcctgga gacagacgat
     2281 ctgcgcaccc tttgccgccg cttccctgat cttgtccatg gccttccgca ggttggccgc
     2341 cttgtcgccg gtacaactca tctgcaccag gcctactttt acattgctca tgacgattac
     2401 cctccggaac tctggtagat atccgaatgg cgcgcaaaat tacactattt gattgggatt
     2461 tcttggtcta tttcctcccc atctgccgca aaaaagccac atgggaagcg accgcatacc
     2521 gcacccgggg atattcgatg taaacaaggc catattcctg gcaggcctgc cggatgatct
     2581 tactgatcgc cgggtagtgt acatgcgaga ttttcggaaa gagatgatgt tctatctgga
     2641 aattcaaccc accaaccagc caggaaacca ccgggttatt ggtggcgaaa ttagccgtcg
     2701 tcttgatctg gtgaacagcc cactcgtcct ccaactttcc ggtgacgacg tcagccttcg
     2761 gaaaagcagt atgttccacc gtatgcgcca attggaacac caggctcaat acaaaccccg
     2821 caacgcaggt aaaaattaca aacccgatga cccagctcga gaacccgatc acatagatcg
     2881 gcagcgcaac aaacaggaaa gcatggaaca gtttgaaccc ccagaaaaca aaatgatcgg
     2941 ccagccccat cctcttcaat ggcatggcgc ccaccttgct cttgaaatat ttctggtaat
     3001 ccagcacgaa gatccaaaag atgtacaaca gggagtaaag aaaccagaaa tacaggtgct
     3061 gatacttgtg cagcttgtat ttcttttgcg tctcgctcat ccgcatccag ggctggatat
     3121 cgatatcgtc gtccaccccg tccacatttg taaatgcatg gtgaatgaca ttgtgtttct
     3181 gattccacat aaagctgttc ccacccagga tattcaggga gaaagcggca aaatgattca
     3241 cccatttgta tttcgaaaag ctgccatgcg ccccgtcatg cattacgtta aaaccaatcg
     3301 ccgcaacgat gcaccccagc agtacgcact cgaggatagc ccaaagaaca cccggggtaa
     3361 agaacacgag gtggatatat acaaacacaa aacttaccat caggatcact gccttgatga
     3421 acaggttgta gttcccggtc tgcgacttcc ccacctcttc gaaataattg ctgatcctct
     3481 tcttcagctc ggtgtggaaa gactgggtgg tgtgggaaaa tttgggcagt gacatgatca
     3541 accggttaaa aatttgaata ataactacaa aggtacctct tccgggtcgg aatccggcgg
     3601 attcaatgtt aaaaacacga caatatcttt aacgaaaatt tcgcttttta agtatccatc
     3661 tgcacgaagg aatcgaaatc ccggacgccg atcatctttt ccgtgatgaa cccctccgca
     3721 tacgggactc cgatcatctt gccgaacatt tgatgtctcc cgatgatggc gttgaggaac
     3781 tccggcgtcg agatataggt ctggggctcg ttctccccat attcggcgga aaagaactgc
     3841 gacgaataag cccgtatcga agccagtttg gtctcgaaaa cgggagtgat atcatacacg
     3901 aagctgggcg cggcataccg gtcctggata taatgaaaga catacttcgg ccgccagtgc
     3961 tcctgcggcc tgccttcatc gtccagcgtc ccgatcttcc gtaatccgct gagaaaacag
     4021 gagtcgctga tcaggtgacc ggcccgcccg tgatccggat gcctgtcttc cagggaattg
     4081 ccaagcacga tctccggcct gtatttgcga attgcccgga taagccgccg ctgatgctcc
     4141 tcgtcattgc ggaaaaaacc atccgccatc cccaggttct cccgcgcatc cagcccaagg
     4201 atcgccgcgg ccttcaccgc ctcctccgcc ctgagctccg cagtacccct cgtccccagt
     4261 tccccacgcg tgagatcgac aaccccggtc ttcttaccat tacgtttttc catcagcaat
     4321 gttcccgcgc aacccagttc cacatcatca ggatgcacgg cgatagcgag tatatcaagc
     4381 ttcatggctg cgaatttacc ccttttttgc attgaggacc aaccattcac cggtttttgg
     4441 ccatatgcgc tttgtgcagt agcttcgcag gtcgcatttt ttaaaacttt aatcaaaaaa
     4501 aacaagcatc agacatggca tacgatttag tcgttctcgg cagcggcacc ggtggctacc
     4561 cagccgccat tcgcgctgca cagctaggct ttaaagtagc aatcatcgaa aaagaaaatc
     4621 tcggcggtgt ctgcctcaac tggggatgta tcccgacgaa ggccttgctg aagagcgccc
     4681 aggtatatca ggatatcctg cattcaaagg aattcggcat cgacgcctcc ggaacggtcg
     4741 atttccccgc cgtcatcaaa cgcagccgcg gggtagccga caaaatgagc aagggcgttc
     4801 agttcctgat gcggaaaaat aagatcgacg tggtcctggg ttatgggaaa ctaaaggcca
     4861 aaggccaggt ggaagtcacc ggcgccgacg gcaagaccca gatcgtggaa ggaaaacata
     4921 tcatcgtagc caccggcgcc cgcagccgcg agctccccaa cctcaaacag gacggccaaa
     4981 aggtcatcgg ctatcgcgaa gccatgaccc ccgctcaaaa acccaatagc atcatcatcg
     5041 tgggcagcgg cgccatcggc gtggagttcg cctatttcta ttacagcatg ggcgcaaagg
     5101 tcactatcgt cgagttcctc ccccggatcg ttcccgtgga agacgaagac atctccaaag
     5161 aactggaaaa gaactataaa aagtacggca tcgatatcct cctcagcagc gaggtcaccg
     5221 gcgtcgatac cagcggcaat ggcgtcaagg caaaggtaaa aactcccacc ggggagatca
     5281 ccctcgaagc cgatgtcctc ctcagcgccg tcggcatctc ggccaatatc gagaacatcg
     5341 gactcgaaac cctcggcatc aagaccgaca aaggcaagat cgtcgtcgac aagttctacc
     5401 agaccagtgt tcccggcatc tatgccgtgg gcgactgcgt ccccggtcag gccctcgccc
     5461 acgtctccac caaggaagcg atcatctgtg tggagaacat tgcctggatg gaaaagaaat
     5521 ttgcccacca gcccgaaatg ctggactata acaatatccc cgggtgtacc tattgcacgc
     5581 ccgagatcgc ttccgccggt ctcacggaaa aacaggcccg cgacgccggc tacgaggtca
     5641 aggtcgggaa attcccgctc agcgcctccg gcaaggccac gggcgccggc cacaccgaag
     5701 gcttcgtaaa agtcgtcttc gacgccaaat acggcgaatg gctcggcacc cacatgatcg
     5761 gctacaacgt gaccgagatc attgc
//
LOCUS       DATGFG010000343         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_370306, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000343 DATGFG010000000
VERSION     DATGFG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370306"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VKQ52_06515"
     CDS             complement(<1..1042)
                     /locus_tag="VKQ52_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HLZ86869.1"
                     /translation="MLLLGIDLGTSSIKVAVTDAATHRVLASAQYPDTEAVIISKQPG
                     WAEQSPDTWWEYVQAAIRRVHASGLYDPLDIAAIGIAYQMHGLVLVDKEQRVLRDSII
                     WCDSRAVPYGDRAFSAIGEERSLERLLNSPGNFTAAKLAWVKELEPAVYERIDKFLLP
                     GDFIALRLTGEATTSISALSEGIFWDFAADELSSDVFRVFGFDKRLVPEIRPLFGAHG
                     GVKADVAASLGLKKGIPVTYKAGDQPNNALSLNVLEPGEVAATAGTSGVIYGVSDALT
                     YDPGSRVNSFAHVNYSPAARRVGVLLCINGTGILNRWVRDLARGEYEELNAAASLVAP
                     GAAGLTVLPFGNG"
     gene            1186..2217
                     /locus_tag="VKQ52_06520"
     CDS             1186..2217
                     /locus_tag="VKQ52_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLZ86870.1"
                     /translation="MTGKKDITIYDIARVLGLSPATVSRGLKDHPAINIKTRKRIMDT
                     AREMGYRSNSFASNLRMQKTNTIGIIVHELKSQFISSVLAGIEKVATEAGYDLIIGHS
                     SETYRKEVSNAHNLFHKRVDGLIASLAFDTSDMDHFDPFVQKGIPIVFFDRVEDFAYG
                     TRVVIDNVRAGYEATAHLAAQGCRHIAHITANLGRNVYAGRLKGYQQALSDYGLAFEP
                     ELLIVNDLSEAAAVRSAKQIIAMHRRPDGVFITNDFFAAVFLQTLKEGGIRVPEDIAI
                     VGFNNDAIARIIQPKLSTINYPGEEMGEQAARSLLEQLSGLSSARTTDTIIIRSELII
                     RESSLHRHT"
     gene            complement(2183..>2437)
                     /locus_tag="VKQ52_06525"
     CDS             complement(2183..>2437)
                     /locus_tag="VKQ52_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ86871.1"
                     /translation="DVQMPEMDGLEATRRIRQGGRRQPAIVALTANAMRGDREDCLRA
                     GMDEYISKPVRLDELMRLLKKWAARPMRDIRYGDGGRIRG"
BASE COUNT          584 a          874 c          529 g          450 t
ORIGIN      
        1 cgccattgcc gaacggcaac acggtcaacc ccgcggcgcc aggcgcaacc aaagacgccg
       61 ccgcattcaa ctcctcatat tccccccggg caaggtcccg cacccaacgg ttaaggatac
      121 ccgttccgtt gatgcaaagc aatacgccca cccgccgcgc cgccggtgaa taattgacat
      181 gcgcaaagct gttgacccgc gaaccgggat cataggtaag cgcatcgctt accccataga
      241 tcacccccga ggtaccggcc gtagccgcca cttcgccagg ctccaacaca ttaagagata
      301 aagcattatt cggctgatca cccgccttgt aggtaacggg gatacccttt ttcaacccca
      361 gcgaggcagc gacatccgcc ttcaccccgc catgcgcgcc aaacaaaggc cggatctccg
      421 gcaccagccg cttatcgaaa ccaaaaaccc ggaaaacatc cgaagacaac tcgtcggcag
      481 caaaatccca gaatatcccc tccgacaacg cagagatgga ggtcgtcgcc tcccccgtta
      541 gccgcagcgc gataaaatcc ccaggcaaca ggaatttgtc gatccgctca tacaccgcag
      601 gctccagctc cttcacccag gccaacttcg cagccgtaaa attccccggg gagttgagca
      661 gcctctccag gctccgctcc tcgccgatgg ccgaaaaggc acggtcacca tacggaacgg
      721 cacggctgtc acaccagatg atgctgtccc gcaacacccg ctgctcctta tccaccaaca
      781 ccagcccatg catctggtaa gctatcccga tcgccgcaat gtccagcgga tcatacaacc
      841 ccgaagcatg tacccggcgg atcgccgcct gcacatactc ccaccaggtg tccggcgact
      901 gctccgccca ccccggctgt ttcgatataa taacggcttc ggtatcagga tactgtgcag
      961 aagccaacac ccggtgagtg gcggcatccg taacagcgac tttaatagag gaagtaccca
     1021 ggtcaatgcc taacaacaac atggagcaat cattttttta attccggccg gtaaatatag
     1081 taccgttttt cgaaattgta accgattgca tcatgaattt taattttaag cgcttatttt
     1141 cgggccctgc gcgcccctgg cgctcattta acctatcgcc tcaccatgac gggaaaaaag
     1201 gacatcacca tctacgacat cgcaagggta ctcggcctct cccccgccac cgtgagcagg
     1261 ggcctgaaag accaccccgc catcaacata aagacgcgca aacggataat ggacaccgcc
     1321 cgggagatgg gctatcgctc caacagcttt gcttccaacc tccggatgca aaaaacaaac
     1381 accatcggca tcatcgtcca cgaactgaaa agccagttca tcagttccgt cctcgccggt
     1441 atcgaaaagg tcgccaccga agccggttac gatctcatca tcggccactc ctcggaaacc
     1501 tatcgcaaag aagtctccaa cgcccataac ctcttccaca aacgcgtcga cggcctcatc
     1561 gccagcctcg ccttcgacac cagcgacatg gaccacttcg atcccttcgt acaaaaaggc
     1621 attcccatcg ttttcttcga tcgcgtcgaa gacttcgcct acggcacccg cgtcgttatc
     1681 gacaacgtcc gcgccggcta cgaagctacc gcccatctcg ccgcccaggg ctgccgccac
     1741 atcgcccata tcaccgccaa cctcggccgg aatgtctacg ccggccgcct caaaggctac
     1801 caacaggccc tctcggatta tggcctcgcc tttgaacccg aactgctcat cgtaaacgac
     1861 ctcagcgaag ccgccgccgt ccgctccgca aaacagatca ttgccatgca ccgccgcccg
     1921 gatggcgtgt tcatcacaaa cgacttcttc gccgccgttt tcctccagac attgaaagaa
     1981 ggcggcatcc gcgtacccga agacatcgcc atcgtcggtt tcaacaacga cgccatcgcc
     2041 cggatcatcc agccaaaatt gagtacgata aattatcccg gtgaagaaat gggagaacag
     2101 gccgcccgca gcctcctcga acaattatcg ggcctttcgt ccgcccgtac gacggacacc
     2161 atcatcatcc gcagcgaatt gatcatccgc gaatcctccc tccatcgcca tacctgatat
     2221 cgcgcatcgg ccgcgcagcc cattttttca atagtctcat caattcatcc agccgcaccg
     2281 gcttgctgat atattcatcc atccccgccc ggagacaatc ctcccggtcc ccacgcatgg
     2341 cattcgccgt caacgccacg atcgccggct gccgcctccc tccctgtctt atccgccgcg
     2401 tcgcctccag tccatccatc tccggcatct gcacatc
//
LOCUS       DATGFG010000344         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_218379, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000344 DATGFG010000000
VERSION     DATGFG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218379"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VKQ52_06530"
     CDS             <1..931
                     /locus_tag="VKQ52_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86872.1"
                     /translation="DGSTASGDPGIPLRTGSSIDTLSARASVKATYNQILNDLHNAEG
                     YLPSDAPGRTLNRPVRISAQALLARVYLSMRNYPMARACADSVLQVYSNLMEYDTLPT
                     TGLFPVSLFNPETLYQASFLGAPSATPYFSAISALYSTTWIDSSLLRSYDTNDLRRVI
                     FYRLKPLDSSCLKGSYAGSYYPFGGLAVDELLLISAECAARAGDATTAMNDINILLSK
                     RWKKGTFNGYNVGSAAEALDTVLLERRKELAFRGLRWTDLRRLNKEGANIQLTRVLNG
                     TTFTLTPNSFNYTLPIPPDVLSLNAAIQQNSRN"
     gene            complement(897..1217)
                     /locus_tag="VKQ52_06535"
     CDS             complement(897..1217)
                     /locus_tag="VKQ52_06535"
                     /inference="COORDINATES: protein motif:HMM:NF039804.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6520 family protein"
                     /protein_id="HLZ86873.1"
                     /translation="MKKIKWSIMSLAVIFSICAAFATKPHFDCTSYPQYYFTGTQYLP
                     VATTYACMQGTITCTYYTTNGGITYSPCAVGTYNGCPGCVVKTPQASATPAVNSGNSV
                     VSQH"
     gene            2061..2471
                     /locus_tag="VKQ52_06540"
     CDS             2061..2471
                     /locus_tag="VKQ52_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HLZ86874.1"
                     /translation="MQQHNTYRLALQLPPLVWSPALARDALAWGQHLAEIDKGQHDPS
                     VRGKEGENLWWGTADAYSYSDMVAMWGNEKNSFKYGVFPDVGTSKSAMVGHYTQIVWK
                     NTTSVGCALVSNGGTDFLVCRYAAPGNVIGEKPY"
     gene            complement(2482..>2963)
                     /locus_tag="VKQ52_06545"
     CDS             complement(2482..>2963)
                     /locus_tag="VKQ52_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HLZ86875.1"
                     /translation="VIGKKLAYHDYGTLTDTLKLDPTRNPSTTQNNPLYFSCNDTLVN
                     IAEKVSRGLILEKPIEGKPAPNIVSGIIVTGDAFISSRTATRRLWQNMHAEATDMEGA
                     AVAQTCWQQGVPFIVIRCLSDAADSEAEKDIARFYRAAAHNAALLVAAITGEIARIR"
BASE COUNT          729 a          690 c          826 g          718 t
ORIGIN      
        1 tgacggctcg acggcgagtg gcgacccggg gatcccgttg cggacgggtt cgagtatcga
       61 tacgctttcc gcccgggcca gcgtaaaggc cacttataac cagatactga atgatctgca
      121 taacgccgag ggctatttgc cgtcggacgc gccgggcagg acccttaatc gcccggtgcg
      181 gatcagcgcc caggccctgc tggcccgggt gtatctcagc atgcggaact acccgatggc
      241 gcgggcctgc gcagatagtg tattgcaggt gtattccaac ctgatggagt atgataccct
      301 gccaacgacg gggctcttcc cggtgagcct gttcaatccc gagacccttt accaggccag
      361 tttcctgggc gccccgagcg ctacgcctta tttttcggct atctctgccc tctattccac
      421 tacctggatc gacagcagcc tgctccggag ctatgacacg aacgatctcc ggcgggtgat
      481 cttctaccgg ctaaagcccc tggattcttc ttgcctgaag ggcagctatg ccggatcata
      541 ttaccctttt ggaggactgg ctgtcgacga gttgctgctg atcagtgcgg aatgcgcagc
      601 gagggcgggg gatgctacga ctgcgatgaa cgatatcaat attttactga gcaagcggtg
      661 gaagaaggga acctttaatg ggtataatgt ggggtctgcg gcagaggcgt tggacacagt
      721 gttactcgaa cgaaggaagg aactggcttt ccggggtctg cgatggacag acctgcggcg
      781 actgaataaa gaaggcgcca acatacaatt gacccgggtt ttaaacggaa caacctttac
      841 gttgacgcct aatagtttca actacacatt gcctatacca cctgatgtac tcagccttaa
      901 tgctgcgata caacagaatt cccggaattg acggctggtg ttgcactggc ttgcggagtc
      961 tttaccacac agccgggaca gccattatag gttccgacgg cgcagggaga ataggtaatc
     1021 ccgccattgg tcgtataata agtgcaagta atagtaccct gcatacaggc gtaggtggta
     1081 gcaaccggga gatattgagt tcccgtaaag taatattgcg gatacgatgt gcaatcaaaa
     1141 tgcggctttg tggcgaatgc ggcacagatg ctaaagatga ctgcaagaga catgatggac
     1201 catttgatct ttttcatacg aaactgtttt tgtttttggt gattgatatg tgattgaagc
     1261 gcttcctacg ttgaaaaact actcccacaa ccgataagac cacaaagaaa aggtttaaag
     1321 ccagatgctg acgccaggtc agtcgtttta tcacaccgcc acaggaacaa ggtatataag
     1381 gaaagaaatg taaaagaatg atgccggtgt agatcgtgaa gagtcccatc actaccagtg
     1441 agccatagaa acctaacagt ctcgtgcgtt cgaagatcag gctaacggat atggctatct
     1501 ccgtgcaagg aatgacccaa acgaggaatg gggtccagga attggggagg ggttgattgt
     1561 tcatctcccc gtagaaggtc ttccagtcaa ggaatttgct tagacttgca tagacgaaaa
     1621 gcagtatcag tagggccgag atacactcca gcaatacctg tcttttgaac atagttacgt
     1681 aggtttggac acgcgatcgt tgcttaggcc aaaattggaa ataaggtaat catgaacgga
     1741 taggaagacg agcattatac gggtcggata agaataaaag gaggataaaa aaggaacagg
     1801 ttattagtaa atgtcggaaa aaaacatcag agtagcaaca tcaatcactg ttaaatttcc
     1861 gtttttttaa ctttttcctg caagcgacgt tagaattttt tctcggctat gaacgttata
     1921 tgtaccgttc tattattcta aaacgaaaaa atatgcgcct taactccttc ttagtgctga
     1981 ttcttgcatc tttcgtctta catgggtatt cacagaatac aaaatcgata tacaatgatg
     2041 cgggctttat ttccgcgata atgcaacaac ataatacgta tcgtttggcc ttgcagttgc
     2101 ctccgcttgt gtggtcgccg gcacttgcga gggatgcgct ggcatggggt cagcatcttg
     2161 ccgagatcga caagggacag catgatccgt ctgttcgcgg aaaagaggga gagaatctgt
     2221 ggtggggaac ggcggacgcc tattcgtatt cggatatggt cgcgatgtgg ggtaatgaga
     2281 agaactcctt taaatatggt gtattccccg atgtggggac gagcaaatcg gccatggtcg
     2341 ggcactatac acagatcgtg tggaagaata cgacatccgt cgggtgtgcc ctggtcagca
     2401 atggcgggac cgattttctt gtttgccggt atgccgcgcc cggtaatgtg atcggggaaa
     2461 aaccctatta agccaatcgg gttatcggat acgagcaatc tcgcctgtga tggctgccac
     2521 gagcagggcc gcgttgtggg cggccgcgcg atagaaccgg gcgatatcct tttccgcctc
     2581 gctgtccgcg gcgtcgctga gacaacggat gacaatgaag gggacgccct gttgccagca
     2641 ggtctgggcc acggcagcgc cttccatatc ggtggcttcc gcatgcatat tttgccagag
     2701 ccggcgggtg gcggtccggc tggagatgaa ggcatctccc gttacaatga tcccgcttac
     2761 gatattgggc gccggttttc cttcgatggg tttctccagg atcaggcccc ggctgacctt
     2821 ttccgcgatg ttcaccaggg tatcgttaca ggaaaagtac agcggattgt tttgggttgt
     2881 cgagggatta cgggtcggat ccagtttcag ggtgtcggtt agcgtcccat aatcatggta
     2941 ggccagtttt ttgccgatga cca
//
LOCUS       DATGFG010000345         5453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_621165, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000345 DATGFG010000000
VERSION     DATGFG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621165"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VKQ52_06550"
     CDS             complement(<1..972)
                     /locus_tag="VKQ52_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLZ86876.1"
                     /translation="MEEQEVQRLRAATGGTAERIHFNNAGSSLPPDVVLEAVTDYLQE
                     EAMQGGYELEAAYKDQLENVYGSIARLINASVDEIAIVENASMGWHLAFNGIPFQKGD
                     VVITSEMEYVTNLIGFLNVKKTKEINIQVIPNDEQGNFSLSALEAAISPAVRLIAMTH
                     IPSTAGNMLPIAEIGKIARRHGILYLVDACQSVGQVPLDVKEIGCDLLSVTGRKYLRA
                     PRGTGFLYVRKEVLEQLGVWFLDGHSVASVTEEGFVLRKDARRFELYEKSRALTIGLG
                     KAIDYALAIGVDRIWQRVRFLAGTLRRRLAEVPGVTVHDSGDQQCGIV"
     gene            1051..1824
                     /locus_tag="VKQ52_06555"
     CDS             1051..1824
                     /locus_tag="VKQ52_06555"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ86877.1"
                     /translation="MNLQRFLPAIPLQPYIREFILIETEEATDSTVIPDTSLVMSLRY
                     KGSVIKKLTEEKELLPHIVVSGLRRSVRNFTYTKQTANFMILFKEGGIAAFSRVPANE
                     LFDQSISADNLFLPAELNQLLERLAEADTDKERLHRVEAFLLRHLASSKQDSLIGNAV
                     RLIRQHNGIIRINHLADSLHLSQDPFEKRFRALVGSTPKQYASIIRLRNLIHNYSSYS
                     SLTEATYDAGYFDQSHFIKDFRLFTGRSPREFFQSPLFW"
     gene            1897..2253
                     /locus_tag="VKQ52_06560"
     CDS             1897..2253
                     /locus_tag="VKQ52_06560"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HLZ86878.1"
                     /translation="MKSYNANPIAAVSPDAPRFIDKFFIPAAAVQEFLERMKINRDFI
                     KKLPGFIRDVAYQHPDGNGNLVVITIAEWESMDAMNKAKDAVQTEYKREGFNPAEMMQ
                     RLNITMDRAIYTQIRD"
     gene            complement(2243..2743)
                     /locus_tag="VKQ52_06565"
     CDS             complement(2243..2743)
                     /locus_tag="VKQ52_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86879.1"
                     /translation="MAAFLTFQKFNDPGLASAIADRLKERRILCHVEKVEPLLEPSFF
                     RNTVEPTIHLKVRATDLDRAHKALEEYYEHQLQDVDPNYYLFSFTDVELLDIVAKPDE
                     WGHFDYVLARELLAQRGLPIPDETIRLMKQQRRRQVAQQVRAESFREFLKRLFRVRSR
                     GKKASP"
     gene            complement(2749..3561)
                     /locus_tag="VKQ52_06570"
     CDS             complement(2749..3561)
                     /locus_tag="VKQ52_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HLZ86880.1"
                     /translation="MKVRPLAFLLFLSACAGHPDSKPTDGTATTVAVPYLNYSVGAVY
                     KHDTTSYTEGLLFHDHQLFESSGATPEDEQTRSIAGIVNLATGKIDTKVELNKDVYFG
                     EGIAFFKNRLYQLTYKNHTGFIYDATTYKRLDTFRFKNAEGWALTTDSNSLIMSDGTS
                     VLTYLDPASLRPVKELAVTESGVALDSLNELEYIKGFIYANRYLKNYIVKIDPASGNV
                     VAKLDLSSLAEAEHVKNPGGDVLNGIAYDRDSDKIYVTGKLWTGIYELNFAH"
     gene            3756..4496
                     /locus_tag="VKQ52_06575"
     CDS             3756..4496
                     /locus_tag="VKQ52_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ86881.1"
                     /translation="MKKLLICILCLASVQGFSQLRLGVQGSFSSLNFWQNDGYTGLDA
                     GENTWAMNGWQAGVFAEYDLGYSGLEIQPALMYATTGAHLGQSKGFADAGTFVIGFSD
                     TKLKINTIRLPVNLLYSFRIDTKWKVFGGLGPYIAKNLSGTEKGYYTGYDNSSNNYVP
                     GTYPINNNIHLSSDQSFNAQGKSNVASLDAGMDILLGFQYKKLQVSASWTRGFSREYY
                     TSYVNMGNVDWNFTLGYILFGHERKPRL"
     gene            complement(4447..>5453)
                     /locus_tag="VKQ52_06580"
     CDS             complement(4447..>5453)
                     /locus_tag="VKQ52_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HLZ86882.1"
                     /translation="AMIDHPLGYYRQAYGLYKMLFGSHPLEAGKEYILFPDGALSLLP
                     VEALVTAADCPSSPAEWPFVIRQTLLSYGWSLQTLSEQRAHPGTASGFAGFFLSGGNG
                     SSPVLAAVSTEEDGIKRVVRDGEWYNDSQATTNRFRHALETSAVVHISSHAFIQKNGV
                     DAPHIELFDDPFYLFELQGLAAHPSLVVLSACRTGDGRIVTGEGVQSLARAFTAGGTN
                     AVVAGWWNLNDETAARLTGNYYSALFADESGNAAAALRQAKLQWLNDPAVGYLQKLPY
                     FWAATGYLGNPRPLEKGFFADGHKQQKQGRWWWPLFLLPAGLFIILAYVRGRRGYNRG
                     "
BASE COUNT         1320 a         1648 c         1267 g         1218 t
ORIGIN      
        1 cacgatgccg cattgctgat ccccactgtc atggaccgtg actcccggaa cctccgccag
       61 ccgccggcgc agcgtccccg ccaggaaccg gacccgctgc cagatacggt ccaccccgat
      121 cgccagcgcg tagtcgatcg ccttgccaag gccaatcgtc agcgcccggc ttttttcata
      181 taattcgaac cggcgcgcat ctttccgcaa aacaaaaccc tcctcggtga cggacgccac
      241 cgaatgccca tccaggaacc aaacacccaa ttgctccagg acctccttcc gcacatacag
      301 gaaacccgta cccctcggcg cccggagata cttgcgcccg gttacagaaa gcagatcaca
      361 accgatctcc ttcacgtcca ggggcacctg ccccaccgac tgacaggcat ccacgagata
      421 caggatgcca tgccgccggg cgatcttccc gatctcggct atcggcagca tattgcccgc
      481 ggtggagggg atatgcgtca tcgcaataag ccgcactgcc ggtgagatag ccgcctcgag
      541 ggcggacaat gagaaattac cctgctcgtc attcgggatc acctgtatat tgatctcctt
      601 ggttttctta acgttaagaa aacctatcag gttggtgaca tactccattt ccgaggtgat
      661 caccacatcc cccttttgaa aagggatgcc gttaaaagca aggtgccagc ccatactggc
      721 gttttccacg atggcgatct cgtccaccga agcattgatc agccgggcaa tggagccata
      781 cacgttctcc agctggtctt tataggcagc ttccagctcg tatcccccct gcatcgcctc
      841 ttcctgcaaa tagtccgtca ccgcctccag gacaacatcc ggcggcaaag aagaccctgc
      901 attgttgaaa tggatccttt cggccgtccc acccgtcgcg gccctcagcc tttgtacctc
      961 ctgttcttcc attgcttgtt tttgagaaca aagtaaagag ttatgtgtat agtaaaacag
     1021 aggcagagtt gctatttttg actatatcag atgaacctgc aaagatttct tcccgccatt
     1081 cccttgcagc cctatatcag ggagttcatc cttattgaaa cagaggaggc caccgacagt
     1141 acggtcattc ccgatacgtc tctcgtgatg tcccttcgct ataagggatc ggtgataaaa
     1201 aaactgacgg aagaaaagga actgctgccc catatcgtcg tctccggcct tcgccggtcg
     1261 gtccggaatt tcacctacac caaacagacc gcgaacttca tgatcctctt caaagaaggc
     1321 ggtatcgccg ctttttcccg ggtgcccgcg aatgagctgt tcgaccagtc catttccgcc
     1381 gacaacctct tcctcccggc cgaactcaat caattgctgg aacgcctcgc tgaagccgac
     1441 accgataaag aacggctcca tcgcgtcgaa gcattcctgc tccgccacct ggccagttca
     1501 aaacaggata gcctcatcgg caacgccgtc cggctgatcc gccagcacaa cggcatcatc
     1561 cggatcaatc acctggcaga ctccctccat ctcagccagg accccttcga aaaacgcttc
     1621 agggccctcg tcggttccac tccaaaacag tatgcctcca tcatccggct tagaaacctg
     1681 atccacaatt actcctccta ttcatcgttg acggaagcca cctacgacgc cggctatttc
     1741 gatcaatccc acttcataaa ggacttccgc ctcttcaccg gccgctcacc aagagaattc
     1801 ttccaatctc ccctgttctg gtagggctct gccagatcaa tgatttttta caatttcccg
     1861 gcgtaagggc aaaatacctt tacaaaaaga aatcaaatga aatcgtacaa cgctaacccc
     1921 atcgcagcag tatctcccga tgccccccgg tttatcgata aattctttat cccggccgcg
     1981 gcagtacagg aattcctcga aaggatgaag atcaaccgcg acttcataaa aaagctgccc
     2041 ggctttattc gggatgtcgc ctatcagcat ccggatggga acggcaatct cgtcgtgatc
     2101 accatcgccg aatgggaaag catggatgct atgaataagg caaaagacgc cgtacagacc
     2161 gaatacaaaa gggaaggctt caaccccgcc gaaatgatgc aacgactgaa cataacaatg
     2221 gatcgggcca tttacaccca gatcagggac tagccttctt ccccctggat ctcacgcgga
     2281 ataatcgctt caggaattcc ctgaagcttt ccgcacgtac ctgctgcgcc acttgccggc
     2341 gacgctgctg cttcatcagc cggatcgttt catccgggat cggcagccct ctctgcgcca
     2401 gtagttcccg ggcaaggaca taatcgaaat gcccccactc atcgggcttg gcgacgatat
     2461 ccagcaattc cacatcggta aaagagaaca ggtaataatt cgggtccaca tcctgcaact
     2521 gatgctcata atactcctca agggccttgt gcgcccggtc caggtcggtg gcccgcacct
     2581 tgagatggat ggtcggctcg acggtattgc ggaaaaaact gggctccagc aaaggctcca
     2641 ccttttcgac atgacaaagg atgcgccgct ccttcaacct gtcggcgatg gccgaagcaa
     2701 gccccgggtc attgaatttt tgaaaggtca gaaaggcggc cataacatct aatgcgcaaa
     2761 attaagctcg tagatccccg tccacaattt tccggtgaca tagatcttat cactgtcccg
     2821 gtcataagcg atgccattga ggacatcccc gcccgggttt ttgacatgtt ctgcttcggc
     2881 aagtgagctc aggtcaagct tggcgaccac attccccgaa gccggatcga tcttcacaat
     2941 atagttcttt aaataccggt tggcgtagat aaaccccttg atatattcca attcattcag
     3001 actgtccagc gcgacaccgc tctcggttac tgccagctcc ttcacgggcc gcagactggc
     3061 cgggtccagg taggtaagga cactcgtgcc gtcgctcatg atcaacgaat tggagtcggt
     3121 cgtcaacgcc catccctccg cattcttaaa acggaaggtg tccagtcgct tatacgtcgt
     3181 cgcgtcatag ataaaaccgg tatggttctt atacgtaagc tggtaaagcc tgttcttaaa
     3241 aaaagcgatc ccctcgccaa aatacacgtc tttgttcaat tccacttttg tgtcgatctt
     3301 cccagtagcc aggttcacaa taccggcgat actcctggtc tgctcatcct ccggcgtggc
     3361 gccgctgctt tcgaacaact gatgatcatg aaagagcaac ccttccgtgt aggatgtcgt
     3421 atcatgcttg taaacagccc cgacactata attaagatag gggactgcca ccgtcgttgc
     3481 cgtaccatcc gttggtttgc tgtcgggatg accggcgcag gcggaaagaa aaagaagaaa
     3541 tgcgagaggc cttactttca tattgatgcg taaaggcaat ttagtgaatt tgccctacct
     3601 tcggcctcgc catagccccg ttcatacgaa cagagcactg gtttgttctt gattatcagg
     3661 attttgacta gttatcggaa tgttaatgtt tcatcacatt gcagcaaaag ggctagtttg
     3721 ccggtcctaa aaaagttcac ccacaataga aacgtatgaa gaaattattg atctgtattt
     3781 tatgtcttgc atccgttcaa ggtttttctc aactccgtct cggcgtccag ggctccttca
     3841 gctctctgaa tttctggcaa aatgatgggt acaccggtct cgatgcaggc gagaacacct
     3901 gggcaatgaa tggctggcag gcaggtgtct tcgcagaata cgaccttggg tattccggtc
     3961 tcgagattca acccgcattg atgtacgcca ccaccggcgc tcacctgggc cagtccaagg
     4021 gatttgccga cgcgggaaca ttcgtcatcg gcttctccga taccaaatta aaaataaata
     4081 ccatccgtct ccccgtcaat ctcctctatt cgttcaggat cgacaccaaa tggaaggtct
     4141 tcggcggcct cgggccctat atcgccaaga acctctcggg tacggaaaaa ggctactaca
     4201 ccggctatga caattcgagc aacaactatg ttcccggtac atacccgata aataacaata
     4261 tccacctctc cagcgatcag tcctttaatg cccagggtaa gagcaatgtc gcctccctcg
     4321 acgcaggcat ggatatcctg ctgggttttc aatacaaaaa actgcaggtc tccgcatcct
     4381 ggacgcgcgg cttctccagg gaatattaca ccagctatgt gaatatgggc aacgtggact
     4441 ggaattttac cctcggttat atcctcttcg gccacgaacg taagccaaga ttataaaaag
     4501 accggccgga agcaaaaaca agggccacca ccatcgtcct tgtttttgtt gtttatgccc
     4561 atcggcaaag aacccctttt ccaacggcct gggattaccc agataaccgg tcgccgccca
     4621 gaaatacggc agcttctgca gatagccgac agccggatcg ttcagccatt gcagcttcgc
     4681 ctgtcttaag gccgcggcgg cattcccgct ctcgtccgcg aaaagggccg aataataatt
     4741 acccgtcagg cgcgccgccg tctcgtcatt aagattccac cagccggcta cgacggcatt
     4801 ggtgcccccg gcggtaaacg cccgcgcaag gctttgcacc ccttctcccg ttacgatccg
     4861 cccatcaccg gtgcggcacg cgctcaacac cacaagggaa ggatgtgccg ccaggccctg
     4921 tagctcgaac aggtaaaaag ggtcatcaaa gagttcgata tggggagcat ccactccatt
     4981 cttctgaata aaggcgtgtg aactgatatg tacgacggcc gatgtttcca gcgcgtggcg
     5041 gaaccggttg gtggtggcct gggaatcgtt gtaccactct ccatctctga ccacccgttt
     5101 tatcccgtcc tcctccgtgc tcaccgccgc aagcacggga gacgatccat tcccgccgga
     5161 aagaaaaaac cccgcgaacc cactcgctgt accgggatgg gcccgttgct cagacaaggt
     5221 ctgcaagctc catccatagg acaacaaggt ctgcctgatc acaaagggcc attcggccgg
     5281 cgaagacgga cagtccgcgg ccgtcaccag cgcttccacc ggcagcaggc taagcgctcc
     5341 gtcgggaaat aagatatact ccttgcccgc ctccagcgga tgacttccaa aaagcatctt
     5401 gtagagccca tacgcttgcc ggtaatagcc gagcggatga tcgatcatcg cat
//
LOCUS       DATGFG010000346         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_597067, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000346 DATGFG010000000
VERSION     DATGFG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597067"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VKQ52_06585"
     CDS             complement(<1..619)
                     /locus_tag="VKQ52_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86883.1"
                     /translation="MKLPAILFTAITVSFCACKGHEKKVLLYASDKIEVDNTKQNITV
                     ANGDGTTHHEQELDFTTGDPVTLNVTSPQGKYTVTIPDDGLYIANLKTDTVVGSRQHV
                     GSEGGEARITQDALKQKLDSLQKLIQGQNVSDANQNYFIVPGKAVKLTTETKAKVFGP
                     FTTIPGSFDAGSVPAIYKFYSMKEMREIIGNLDKMMTKETAPTDSA"
     gene            complement(667..1272)
                     /locus_tag="VKQ52_06590"
     CDS             complement(667..1272)
                     /locus_tag="VKQ52_06590"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HLZ86884.1"
                     /translation="MAFTLPALPYAFDALEPNIDTTTMQIHHGKHHQAYIDNLNKAIA
                     GTPNEGKSLEELVQKAGSISPAVRNNAGGHWNHSFFWEILSPKSTGAPSGKLADEIHT
                     TFGSFDAFKEKFSAAGMTRFGSGWAWLIVKDGKLEVTSTPNQDNPLMDVAEVKGTPIL
                     GVDVWEHAYYLKYQNKRADYLAAFWKVVNWDKVAQHYSAAK"
     gene            complement(1398..1859)
                     /locus_tag="VKQ52_06595"
     CDS             complement(1398..1859)
                     /locus_tag="VKQ52_06595"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HLZ86885.1"
                     /translation="MILQPMNDEYFMQQALKEARLAYEEDEVPIGAVIVMGNRVIARG
                     HNQTERLTDCTAHAEIIALTSAFNHLGSKYLPEAALYVTIEPCLMCAGALYWSKIGRV
                     IFGAEDEKNGYRRGIGAQPAPWPFHPRTELVGGVLSEDCAALMRDFFRSKR"
     gene            1905..3287
                     /locus_tag="VKQ52_06600"
     CDS             1905..3287
                     /locus_tag="VKQ52_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HLZ86886.1"
                     /translation="MNEPVLSIDAEPLRRFFQSGATRSYAWRRQQLRLLQEAIRAEET
                     TIHQALYADLKKSPEEAYATETGLVLAEIRHTLKHLAKWMAPQRAGTNLVNLPAASYV
                     YRDPLGVVLVIAPWNYPLMLSLVPLAAALAAGNTVVLKPSELAPSTAAVIEKLLTRIF
                     PPELVRVVQGDGATVVPGLIRQFRFDHIFFTGSVPVGQAIYRLAAEQLIPVTLELGGK
                     SPVIVGKDANLRVAARRIVVGKFANAGQTCVAPDYLLVHADVQEALLSALPETINDFY
                     GAQPRDSYDYGRIINGKRFDTLVGYLADGGIFCGGDHDRAALYIGPTLLTGVSPDAPV
                     MREEIFGPLLPVFAYRTTEEAMAFVRRYPDPLAFYLFTADKGLQKAWIEGVSFGGGCI
                     NNTDWHFANPHLPFGGVGKSGIGAYHGKYSFDVFTHAKGVMRTPTLIDPAIKYPPFKG
                     KMKWFKRFIR"
BASE COUNT          608 a          869 c         1036 g          813 t
ORIGIN      
        1 gcgcgctatc ggtaggggca gtttcttttg tcatcatctt gtccagattg ccgatgatct
       61 cgcgcatttc tttcatgcta tagaacttat agatggcggg gacggagccg gcatcgaatg
      121 aaccggggat ggtcgtaaag ggaccgaaga ccttggcctt tgtttcggtg gtgagtttta
      181 ccgccttgcc ggggacgatg aagtagttct ggttggcatc gcttacgttc tggccctgga
      241 tgagtttctg gaggctgtcg agtttttgtt tcagtgcgtc ctgggtgatg cgggcttcgc
      301 cgccttcgga accgacgtgc tggcggctgc cgacgacggt gtccgtcttg aggttggcga
      361 tatacaggcc gtcatcgggg atcgtgacgg tgtatttgcc ctggggggag gttacgttga
      421 gggtcaccgg gtcgccggtg gtgaagtcca gttcctgttc gtggtgcgtg gtgccgtcac
      481 cgttagctac ggtgatgttt tgcttcgtgt tgtctacttc tatcttatcg ctggcgtaga
      541 ggaggacttt tttttcatgt cctttacagg cgcagaagga gacggtgata gcggtgaaca
      601 ggatagctgg taatttcata tttggttttt tagagccggc gccgcaggcg ccggctcttt
      661 ttggaattat tttgcggctg aataatgttg cgctacttta tcccagttaa cgactttcca
      721 gaaggcagcg agataatcgg cgcgtttgtt ctggtatttc aggtaatacg catgttccca
      781 gacgtcgacg cccaggatgg gggtgccttt tacttcggct acgtccatca gtgggttgtc
      841 ctggttgggg gtggaggtga cctccagttt tccgtccttt acgatcagcc atgcccagcc
      901 gctgccgaaa cgggtcatac ccgcggcgct gaacttttcc ttgaaggcgt cgaagctgcc
      961 aaaggtggta tggatctcgt cggccagctt cccggaagga gcacccgtgg atttggggct
     1021 caggatctcc cagaagaagc tgtggttcca gtgaccgccg gcgttattac ggacggccgg
     1081 gctgatggag cctgctttct gaaccagctc ttcgagggat ttgccctcat tcggcgtgcc
     1141 ggcgatggcc ttatttaaat tgtcgatata ggcctggtgg tgttttccat gatggatctg
     1201 catggtagtg gtgtcgatgt tcggctccaa tgcatcaaat gcataaggca gcgcgggaag
     1261 tgtaaatgcc ataacgaatg gtttaatgat attaaaattt gctaatgtat gaccttcggt
     1321 cgagccgcct ccggcggccc tggttacgtt cgtgtcaatg cctgaaggaa aacaaagcta
     1381 atgcaaaaag gttaggatca gcgcttcgaa cggaaaaaat cccgcatcag cgcggcgcaa
     1441 tcctcgctga ggacgccccc taccagttcg gtcctgggat gaaaaggcca gggtgcgggt
     1501 tgtgccccga tgccccggcg atagccgttc ttttcatctt cggcgccaaa gatgacgcgg
     1561 ccgatcttgc tccagtacaa tgcgccggcg cacatcaggc agggttcgat ggttacgtat
     1621 aacgcggctt cgggcaggta tttgctgccg agatgattga aggcggaggt cagtgcgatg
     1681 atctcggcat gtgcggtgca gtcggtgagc cgttcggtct ggttatgacc gcgggcaatg
     1741 acccggttgc ccatgacgat gacggcgccg atgggaacct cgtcctcttc ataggccagg
     1801 cgtgcctctt tcagggcctg ttgcataaaa tattcgtcat tcatcggctg caagatcaca
     1861 aaatttagat tatttttcga ccattttttt gccgaaattc aggcatgaac gagcctgtcc
     1921 tgtccatcga tgccgaaccg cttcgccgct tctttcaatc cggtgcgacg cggtcgtatg
     1981 cgtggcggcg gcagcaactg cggctgctgc aggaggcgat ccgggccgag gagaccacga
     2041 tccaccaggc cctttatgcc gatctgaaga agagtcccga agaagcctat gccacggaga
     2101 cggggcttgt gctggcggag atccggcaca ccctgaaaca ccttgcgaaa tggatggcgc
     2161 cgcaaagggc gggcaccaat ctcgtcaacc tccccgcggc ctcctatgtc taccgggatc
     2221 cgcttggggt cgtactggtg atcgccccct ggaattatcc tcttatgttg tcgctcgttc
     2281 cgcttgccgc ggcccttgcc gccgggaata ccgtcgtgct gaagccttcg gagctggcgc
     2341 cctccacggc cgccgtgatc gaaaaactcc tcacccgcat cttcccgccg gaactggtcc
     2401 gggttgtcca gggcgatggc gcaacggtgg tacccgggct gatccggcaa ttccgctttg
     2461 atcatatctt cttcaccggt agtgtccccg tggggcaggc gatctaccgt ttggctgcgg
     2521 aacagcttat ccccgtgacc ctcgaactcg gcggcaagag ccctgtcatc gtcgggaagg
     2581 acgccaacct gcgggtggcg gcgcggcgca tcgtcgtcgg taaattcgcc aatgcgggcc
     2641 agacctgtgt cgcgccggat tacctgttgg tgcatgccga tgtgcaggaa gccctgctta
     2701 gcgccttacc ggagacgatc aacgattttt atggcgcaca gccgcgggac agttatgact
     2761 atgggaggat catcaacggg aagcgattcg acaccctggt cggctacctc gcagacggag
     2821 gcatcttctg cggcggcgac catgaccgtg cggcgttgta tatcgggcct accctgctga
     2881 ccggggtttc gcccgatgcg ccggtgatgc gggaggagat cttcggtccc ctgttaccgg
     2941 tatttgctta ccggacgacg gaggaggcca tggcctttgt ccggcgttat cccgatccgc
     3001 tggcctttta tctttttacg gcggacaagg gattgcaaaa ggcatggatc gaaggggtct
     3061 ccttcggggg tgggtgtatc aataataccg actggcattt cgccaacccg cacctgccgt
     3121 ttggcggggt cgggaaaagc gggatcggcg cttatcatgg aaaatattct ttcgatgtct
     3181 ttacgcatgc caagggggtg atgcggacgc cgaccctgat cgatccggcg atcaagtatc
     3241 cgccgttcaa ggggaagatg aagtggttca aacgatttat ccggtgaggg ggtgatgccc
     3301 gggggcggtg gggttgggct ttcgcg
//
LOCUS       DATGFG010000347         3499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_255691, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000347 DATGFG010000000
VERSION     DATGFG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255691"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VKQ52_06605"
     CDS             complement(<1..802)
                     /locus_tag="VKQ52_06605"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A-modifier LpxR family protein"
                     /protein_id="HLZ86887.1"
                     /translation="MIRTLLMLFIAGELLTATSRSWAQPPTTRTKDSVTRLIRAYEDD
                     DYINFWGCGTDNAYTNGSRLDYFYLPDHRPHGVLGRFAPRAGDSSIDIYGWGVMEIMY
                     TPNNITKTEWQPDDYQYAGALVAIHSRYSYNPVKKYDFQTELVMGIIGPSAMAKQLQS
                     GFHHLIHYTQPMGWGHQFRNDALLNVNFTAEKQLAAAGDWLTIIGGAQAYAGTMQNGA
                     ALYPLILIGRMNPYFDGFFSEHVSNDRKKWQGYLLFRPELQYFAQNALL"
     gene            complement(1074..2162)
                     /locus_tag="VKQ52_06610"
     CDS             complement(1074..2162)
                     /locus_tag="VKQ52_06610"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A-modifier LpxR family protein"
                     /protein_id="HLZ86888.1"
                     /translation="MNRSLLILFFLSGLTLVTVRPMAQYRATTYKDSVTSLLRVSEDN
                     DGINIFFQSTDDAYTNGTRLDYFYKPGRRLHGLLGRWAPGAGEGSVDIYSWGLMQLMY
                     TPNDMSQTVYQPNDYPYSGALVATHTRYSYNPVKKYDLQTEVVMGALGPLSLAHQTQS
                     LVHRLTGFNQPMGWGTQFHNALLLNVNLTAEKQIAAAGQFLRIIGGGQVYAGTMQNGV
                     AIYPLILIGKMNPYFDGLFSQYTSPGRDERGRKKWQFYFLAKPELQFYLTNALLEGGI
                     FTTNPNKKSSDNKNIPQPVSTGYSQQPPPPPPPSIPSLQRLVSSFSYGGVLSHGGFAI
                     SFVQNVSSTTLKGLYCHDFGNVSLYFGW"
     gene            complement(2191..>3499)
                     /locus_tag="VKQ52_06615"
     CDS             complement(2191..>3499)
                     /locus_tag="VKQ52_06615"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HLZ86889.1"
                     /translation="GQQGNFLDVPTDCPQRDERLGWTGDAQVFSRTAAYNRGVDNFFA
                     KWLKDVAADQQSTGAVPFVIPNVAGNGTSAGWADVATIIPWNIYLAYGDRRVLADQYP
                     SMKAWVEYIRGQAKNDLWNTGSHFGDWLFYRPFDDNDGRSAVTDKYLIAQCFYAHSTQ
                     LLIDAATVLGKKEDVDGYTTLLKKIKDAFLREYMTPEGRLVSGTQTAYVLALQFDMLP
                     EAFRKQAAERLVKNIQDYNYHLTTGFLGTPYLCHVLSRYNFTAVAYRLLLQDTYPSWL
                     YPVKMGATTIWERWDGIKPDGSFQTPGMNSFNHYSYGAIGDWMYRVVAGIDTYEDGPG
                     YHHSRISPHPGGGLSYAYGDLETGYGRISSHWQLTDDSLTIDLVIPANTTSTVYIPAP
                     EGGLITEGGHNLSTVKDIRIEGIEKGYLLVRLGSGNYHFRLSQ"
BASE COUNT          767 a          926 c          972 g          834 t
ORIGIN      
        1 ccagcagcgc attttgtgca aagtattgca gctcgggtct gaacagcaga tatccctgcc
       61 atttcttcct gtcgttgctg acatgctcgc tgaaaaaacc gtcgaagtaa gggttcatcc
      121 ggccgatcag gatcaaggga tacagggctg ctccgttctg catcgttccg gcataggcct
      181 gcgctccacc aatgatggtt agccagtcac ccgcggcggc caactgcttc tccgccgtaa
      241 aattgacatt cagcagggca tcgttccgga actgatggcc ccagcccatg ggctgggtat
      301 aatggataag gtgatggaag ccggattgga gctgtttcgc cattgcggaa gggccgatga
      361 tgcccatcac cagttccgtt tgaaaatcat atttcttcac gggattgtaa gaataccggg
      421 agtggatggc taccagcgcg cctgcgtact gataatcgtc gggctgccat tctgttttgg
      481 tgatattgtt cggagtatac atgatctcca tgacccccca gccataaata tcgatgctgc
      541 tgtcccccgc ccgcggcgcg aagcggccga ggacgccgtg cggccgatga tccggcaaat
      601 aaaaataatc cagccggctt ccgttggtgt aggcattatc cgttccgcaa ccccagaaat
      661 tgatgtagtc atcgtcttcg taagcccgga tcaggcgcgt tacgctatct tttgtccttg
      721 tcgttggggg ttgtgcccag gagcggctcg tggccgtcaa tagctccccg gcaataaaga
      781 gcatgagtaa agttctgatc atatcctgtc tgtttagctg ttattcttct attgagtcgt
      841 ctttccatac gaatggtgct gtaaacaaac aaaggatgcc cttaccagcc agggaagccg
      901 gaataattta tcggacgggc ttgtagtgtg aagttaccgg gttggagatg ggagctcgtc
      961 tttgtttgtc ctatcgtcta tgactttacc tgttaatctt atcctatttt ttcttcttcg
     1021 atgtatatat taccgatcag ccggcaaaag gttcggtcgg tgaaaaaatt tttttaccag
     1081 ccgaagtaaa gggagacatt gccaaaatca tgacaataca atccctttag ggtggtggaa
     1141 gaaacatttt gaacaaagga gatcgcaaaa ccgccgtgcg aaagaactcc gccataggag
     1201 aaggaactga ccagtcgctg cagggaaggg atggatgggg gtgggggagg cgggggctgt
     1261 tggctgtagc cggttgacac cggctgaggg atattcttat tgtcggaaga cttcttgttt
     1321 gggttggtcg taaagatccc gccttccagc agcgcgtttg tcaggtagaa ctgcaattcg
     1381 ggttttgcca ggaaatagaa ctgccatttc ttccggcccc gctcgtcccg tccgggactg
     1441 gtgtactggc tgaaaagacc gtcgaaataa gggttcatct tcccgatcag gatcagcgga
     1501 taaatggcca ctccattctg catcgttccg gcatagacct ggcctccgcc gatgatccgc
     1561 aggaactgcc cggcggcagc gatctgcttt tccgctgtga gattgacatt cagcagcagc
     1621 gcattgtgaa actgcgttcc ccatcccatg ggttgattga agccggtgag ccggtgaacg
     1681 agggattggg tctgatgggc cagggatagc ggtcccaatg cgcccatcac gacctccgtc
     1741 tggagatcgt acttttttac agggttatac gaatagcggg tatgtgtggc gaccagcgca
     1801 ccggaatagg gataatcgtt tggctggtat acggtctggc tcatgtcatt cggcgtgtac
     1861 atcagttgca tcaggcccca gctatagata tcgacgctgc cctcgccggc gccgggcgcc
     1921 catctgccca gcagtccatg gaggcggcgg ccgggcttgt aaaaataatc cagccgcgta
     1981 ccattggtat aggcgtcatc cgtcgactga aaaaagatat tgatcccgtc attgtcttca
     2041 gatacgcgca gcaggctggt tacactgtcc ttataggttg tcgcccggta ttgcgccatc
     2101 ggtcgcaccg ttaccagcgt caggcccgaa aggaagaaga gaattagtag agatcggttc
     2161 atatccagtc tgtttgactc atcgattgtt ctattgtgac aaccggaaat ggtaattgcc
     2221 cgatcccagc cggacgagca ggtatccttt ttcgatccct tcgatgcgga tgtctttcac
     2281 cgtagacagg ttatgaccgc cttccgtaat aagcccgccc tccggcgcgg ggatatacac
     2341 ggtcgatgtg gtattggccg ggatcaccag gtcgatggtc agggaatcgt cggtcagctg
     2401 ccagtggctg ctgatcctgc catacccggt ctccaggtcg ccgtacgcat agctgagccc
     2461 gccgcccgga tgtggcgata tccggctgtg atggtagccg gggccatcct cataggtgtc
     2521 gataccggct accacccggt acatccagtc cccgatggcg ccataagaat agtgattaaa
     2581 ggaattcatg cccggggtct ggaagctgcc atccggttta ataccgtccc agcgttccca
     2641 gatcgtcgtc gcccccatct ttacggggta caaccaggag ggataggtat cctgcaatag
     2701 cagccggtaa gccaccgcag taaaattgta ccggctcaat acatgacaga gatagggtgt
     2761 gcccaggaac ccggtcgtaa gatgataatt ataatcctgg atattcttta ccagtcgttc
     2821 agcggcctgt ttgcggaacg cctcgggcag catatcgaac tgcagggcca gcacataggc
     2881 cgtttgcgta cccgatacca accggccttc gggggtcata tattcccgta aaaaggcatc
     2941 cttgatcttt ttcaacaggg tcgtataccc atccacgtct tcctttttcc caaggacggt
     3001 agccgcatcg atcagcagtt gggtcgaatg ggcataaaag cattgcgcga tgagatattt
     3061 atccgttacc gccgagcggc cgtcattgtc gtcgaagggc cggtagaaaa gccagtcgcc
     3121 gaagtgagaa ccggtattcc agagatcatt tttggcctgc ccgcggatat attccaccca
     3181 ggccttcatg ctggggtact gatctgccag cacccgtctg tcgccatagg ccagatagat
     3241 attccaggga atgatcgtgg cgacatcggc ccagccggcg ctcgtgccgt tgccggcgac
     3301 attgggaatg acaaagggaa cagcgcccgt ggactgttgg tccgcggcga catccttcag
     3361 ccatttggca aagaaattat ccaccccgcg gttgtaggcg gcagttcggg aaaacacctg
     3421 cgcatcgccc gtccagccca atcgctcgtc gcgttgcgga cagtcggtcg gcacatccag
     3481 gaaattgccc tgctgcccc
//
LOCUS       DATGFG010000348         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_406127, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000348 DATGFG010000000
VERSION     DATGFG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406127"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VKQ52_06620"
     CDS             <1..1650
                     /locus_tag="VKQ52_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLZ86890.1"
                     /translation="YAINFQPFTIIPTGKIGLVLARDGAELETGSILARKVPCDSFQD
                     AAAFLRSGGRKGRQTAFMTPGSYRINTFLFDVEITDMISIPDNAVGIVTTLEGKPLDE
                     GQIAGRVIGDHNKFQDADFFLNNGGYKGLQEQVILAGSYFLNPWFVKVDMVKMTEIPI
                     GYVGVVISFVGSEGADLSGVDFKHGNIVARGNKGVWADPLGPGKYPINPYIQKTELVP
                     TTNLVLNWASARSEAHQLDKNLSTITVRSRDGFPFNLDVAQIIHIPTTEAPKVIARFG
                     NMVNLVSQVLEPTIGNYFRNSAQDSDVIAFLGTRKERQESARLHIGRVLEQYNVIGVD
                     TLIGDIVPPDSLMKTLTDRKLAEEQKVTYDTQMKAQETRQSLEKETAIADIQKEIVKA
                     DQGVLIAERIADASVKKATGDANSVRLQANAEADRVKLLASGEAEKTRLLAKADAEKI
                     EWLAKADAEKISLTGNAEAEKILAIGKSSAESYKLAVEAMGGGNFTQLKVMEAIGQQH
                     IRIMPDILIGGGGEGANGPINGLLGLQLLEQLKKKTDEKDN"
     gene            complement(1647..2087)
                     /locus_tag="VKQ52_06625"
     CDS             complement(1647..2087)
                     /locus_tag="VKQ52_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86891.1"
                     /translation="MIRPVLLLAAVLFYGMDQDVKVATWSTGKPDTDSYESLSFWIKE
                     NHRAYVRYAHGKGEVDTELQWLGPDSYNGRRAFRVTSPQTGACCWVIIPDSARIEVID
                     RQTNSTRSFYWEDENPAGDSTTACAICAPNEKEAQGLLRRYFMR"
     gene            2113..>3051
                     /locus_tag="VKQ52_06630"
     CDS             2113..>3051
                     /locus_tag="VKQ52_06630"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HLZ86892.1"
                     /translation="MFSLRSSAFFLLCTGYFLLGAPLIGEGQTGAAVPAAGVPFGMAR
                     QRAAILRDIRYDLAFHLPADKENPVHGEETLVFTLGKDPRAPLPLDFKAPTGAVLSLR
                     VNGAAAPVRLEKEHLLLPPAFLHRGPDTVTVSFIAGNAALNRNSEFLYTLLVPDRART
                     LFPCFDQPDLKAIYRLTLTSPAGWKVISNAPLLDSTDWGDSCRFRFGPSDRLSTYLFS
                     FAAGRFSATTRMVDGRPMRFLYRETDSVRLRLSLDSVFRIEGQALRFLQQYTASSYPF
                     QKFDFVAIPDFQFGGMEHAGAIQYKASSLFLDSGATR"
BASE COUNT          659 a          907 c          880 g          605 t
ORIGIN      
        1 tatgccatca acttccagcc ttttacgatt atcccgacag gaaagatcgg cctggtgctg
       61 gcccgcgacg gcgcggagct cgaaacgggg tctatcctgg cgcgtaaggt tccctgcgac
      121 tccttccagg atgccgcggc atttctgcgg agcggcggac ggaaaggccg ccagacggcc
      181 ttcatgacac ccggctccta tcgtatcaac acctttctct tcgacgtgga gatcaccgat
      241 atgatcagca tccccgacaa tgccgtcggc atcgtgacga cgctcgaggg aaagcccctc
      301 gatgaaggac agatcgccgg ccgtgttatc ggcgatcaca ataagttcca ggatgccgat
      361 ttcttcctga acaacggcgg ctataagggc ctgcaggagc aggtgatcct cgccggttcc
      421 tatttcctga acccctggtt cgtcaaagtg gacatggtga agatgaccga aatacccatc
      481 gggtatgtcg gcgtcgttat ctcctttgtc ggcagcgaag gggccgatct cagcggcgtg
      541 gacttcaaac atggcaacat cgtcgcccgc gggaataagg gcgtatgggc cgatccgtta
      601 gggcccggta aatacccgat caacccctat atccagaaaa cggagttggt gcccaccacc
      661 aacctcgtgc tcaactgggc cagcgcccgc agtgaagcgc atcagctgga caagaacctg
      721 tcgaccatca ccgtgcgcag ccgggacggc ttccccttca acctcgacgt agcgcagatc
      781 atccatatcc cgacgacgga ggcgccaaag gtgatcgccc ggtttggaaa tatggtgaac
      841 ctcgtgagcc aggtgcttga acctaccatc ggtaactatt tccgtaactc cgcacaggac
      901 agcgatgtca tcgccttcct gggcacgcgt aaggaacggc aggaatcggc cagattgcat
      961 atcggcagag tattggagca atacaatgtc atcggtgtcg acaccctcat cggtgatata
     1021 gtgccgcccg atagcctgat gaagaccctg accgaccgga aactggccga agaacaaaag
     1081 gtgacctatg atacccagat gaaggcccag gagacccggc agagcctgga aaaggaaacg
     1141 gctatcgccg atatccagaa ggagatcgta aaggccgacc agggcgtgct catcgccgaa
     1201 cgcatcgccg acgcttccgt gaagaaagcc accggtgatg ccaacagcgt caggctccag
     1261 gcgaatgctg aggccgatcg ggtcaaactg ctggccagcg gtgaagccga aaagacccgt
     1321 ttgctggcga aggcggatgc ggagaagatc gaatggctgg caaaagccga tgccgagaag
     1381 atcagtctca ccggtaacgc ggaagccgaa aagatcctgg ccatcggtaa gtcttccgcc
     1441 gaatcctata aactggcggt ggaagcgatg ggcggtggca atttcaccca gttgaaggtc
     1501 atggaggcga ttggtcagca acatatccgg atcatgcccg atatcctgat cggtggcggc
     1561 ggcgagggcg caaacggccc cattaacgga ctgctgggac tgcaattgct ggaacaactg
     1621 aagaagaaaa cggatgagaa ggataattaa cgcataaagt agcggcgaag caggccctgg
     1681 gcttccttct catttggcgc gcagatagcg caggcggtgg tagagtcgcc ggccggattt
     1741 tcatcttccc aataaaagga cctggtggag ttcgtctggc gatctatcac ttctatacgg
     1801 gcactatcgg ggatgattac ccagcagcag gcgcccgtct gtggggaagt tacccggaag
     1861 gcccgccggc cgttatagct atccggtcca agccattgga gctcggtatc cacttccccc
     1921 ttgccatggg cataacggac ataggcgcgg tggttctcct ttatccagaa ggaaaggctt
     1981 tcataactgt cggtatccgg ctttccggtg gaccaggtgg ctactttaac atcctgatcc
     2041 ataccgtaaa aaaggaccgc cgccagcagc aggacaggtc tgatcatgtc ccaaaatacg
     2101 atatattcgc ccatgttcag cctgcgatca agtgcttttt tcctgctgtg cacagggtat
     2161 tttctcttag gagcacccct catcggcgag gggcagacgg gtgccgcggt gcccgcggcc
     2221 ggcgtccctt tcggcatggc ccgacagcgg gccgcgatac taagggacat ccggtatgat
     2281 ctggcttttc atctgcccgc ggataaggag aatcccgtac acggggagga aaccctcgtc
     2341 ttcacgcttg gcaaagatcc ccgggccccg ctgccgctcg acttcaaggc ccccaccggg
     2401 gccgtcctgt ccctgcgggt caacggcgcc gccgcgcccg tccggttgga gaaggaacac
     2461 ctgctgctcc cgccggcctt ccttcaccgg ggccccgaca cggtgacggt atcgttcatc
     2521 gccggcaatg cggcgcttaa ccgtaacagt gaattcctct atacgctgct ggtgcccgac
     2581 agggcccgga ccttgttccc ctgtttcgac cagcccgatc tgaaggccat ataccggctt
     2641 accctcacct cgcccgcggg gtggaaagtg ataagcaatg caccgctgct ggattcgacc
     2701 gattgggggg atagctgtcg tttccgcttc ggtccctccg atcggctcag cacctatctc
     2761 ttttcttttg cggcgggccg gttttcggca accacccgga tggtagacgg ccggccgatg
     2821 cgcttcctat accgcgagac cgacagcgtc aggctccggc tcagcctcga ctccgtcttc
     2881 cggatcgagg ggcaggccct gcgttttttg cagcagtata ccgcgagcag ctatcccttc
     2941 cagaaattcg actttgtcgc catacccgat tttcagttcg ggggcatgga acatgcgggc
     3001 gcgatccagt ataaggcaag ttctcttttc ctcgattccg gcgccacgcg g
//
LOCUS       DATGFG010000349         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1031250, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000349 DATGFG010000000
VERSION     DATGFG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031250"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VKQ52_06635"
     CDS             <1..605
                     /locus_tag="VKQ52_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HLZ86893.1"
                     /translation="AQGKMDLILDNLIADGKAKPMLVVMDRGYATDPTGHTRPPASGM
                     PGGMNDNIFPEVLVKEIIPMVDSAFRTIPDRDHRAMAGLSMGGFQTFQTTMTNLDKFA
                     YIGGFSGAAFLQPGTDITRLYNGAWADAPAFAQKVKVLYLSVGTAEPPRMLTGIRGLH
                     DALEKAGIRHVYYESPGTAHEWQTWRRSLRQFAGLIFKQP"
     gene            complement(608..1483)
                     /locus_tag="VKQ52_06640"
     CDS             complement(608..1483)
                     /locus_tag="VKQ52_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ86894.1"
                     /translation="MNKRVMGHLFSLLHIDYVRLDNKWNYANVISPYFRIYYIDEGAG
                     SILGAGDKCRLEPGFIYMIPSFTLCHLRCEDHLGQYFLHFFEESATGISLFEYNRKVM
                     RERAREADILNFRRLLEINRGRGINRSDDPKVYEKNAYYRDYQQLNNTVSDAVYLETQ
                     GIILQLISRFLRSQHFKPNYPAPMPSKVLDAIGYIQLNLHDELTVCDLAKRANLHEDY
                     FSRLFVRFTGERPLNYIHVKRIQRAQYLITTTNMSYSQIAEETGFANLPYFSKIFKKV
                     TKLTPAEYKRSNERV"
     gene            1637..>2399
                     /locus_tag="VKQ52_06645"
     CDS             1637..>2399
                     /locus_tag="VKQ52_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HLZ86895.1"
                     /translation="MTTNSNGEKAVLVTGGSGFIAVHCILQLLNAGYRVKTTVRSLTR
                     EPEVRAMLKVGGIEAGEHLSFIEADLGSDTNWDKAAGGCTYILHVASPTPLKAYKHED
                     EMIIPAREGVLRVLRSARNAGVRRVVLTSAFGAIVYGHKPQLTPFTEKDWTNTTGKVP
                     PYQKSKTLAERAAWDFIGKEGGQLELSVVNPVTVLGPVLGPDYSHSIHLIKNLITGSM
                     PGCPKINSAFVDVRDVADLHLRAMTHPAANGERFIA"
BASE COUNT          575 a          650 c          636 g          538 t
ORIGIN      
        1 gggcgcaggg gaaaatggac cttatcctcg acaacctcat tgcggatggg aaggcgaagc
       61 ccatgctcgt agtcatggac aggggatacg ccactgaccc taccggccac acgcgccctc
      121 cggcctccgg aatgccgggc ggtatgaacg acaatatctt ccccgaagtg ctggtaaagg
      181 agatcatccc gatggtcgac agcgcattca ggaccattcc cgaccgcgat caccgggcca
      241 tggcgggatt gtcgatgggg ggcttccaaa cctttcaaac caccatgacg aacctcgaca
      301 agttcgccta catcggcggt ttcagcggag ccgccttttt acaacccggt accgatatta
      361 cccggctata caacggcgcc tgggcggatg ccccggcctt cgcccagaag gtaaaggtgc
      421 tgtacctgag cgtgggaacg gccgagcctc cccggatgct cacaggcatc aggggcttgc
      481 atgacgccct ggaaaaagcg ggcatccggc atgtttatta tgagtcgccg gggacggccc
      541 acgaatggca gacctggcgg cggagcctgc ggcaatttgc cggcctgata tttaaacagc
      601 cttgagctta tacacgctca ttcgagcgct tgtattccgc gggcgtgagt ttcgtcacct
      661 ttttaaagat cttcgagaaa tagggcaggt tggcaaatcc ggtctcttcc gctatctggg
      721 aataagacat attggtcgta gtgataagat actgcgcccg ttggattcgt ttgacatgga
      781 tatagttcaa cggtctctca cccgtgaatc gcacgaataa tcgcgaaaaa tagtcttcgt
      841 gcaggttggc tctttttgcg agatcgcaaa cagtaagctc gtcatgcagg ttcagctgga
      901 tatagccgat cgcatcgagg accttcgagg gcatcggcgc cggatagttt ggcttgaaat
      961 gctgcgaacg caggaagcgg gaaatgagtt gcaggatgat gccctgcgtc tccaggtaaa
     1021 cggcatcgct gacggtattg ttcaattgct ggtagtctct gtaataagca ttcttctcat
     1081 acaccttcgg gtcgtccgac cggttgatgc ctcttccccg gttgatctcc agcagtcgtc
     1141 gaaagtttag gatatccgcc tcccgcgccc gttccctcat cactttccgg ttgtattcga
     1201 aaagagagat ccctgtcgcc gactcctcga aaaaatggag gaaatattgg ccgagatggt
     1261 cctcgcagcg aagatggcat aaggtaaagc tcgggatcat gtaaataaag ccaggctcca
     1321 gccggcattt atccccggcg ccgaggatcg atcccgctcc ttcgtctata taatagatcc
     1381 ggaaataagg actgatcaca ttggcatagt tccacttatt atcgagcctg acatagtcga
     1441 tatgcaacag ggagaataga tgtcccatga cccgcttgtt catagacgct ttttttcaag
     1501 gtacgaaaaa gtcggatttg tataggccgc acgctttaca aaataccccg gcttttaaac
     1561 aaagtatatt gcgccttttg aacaaagcga ataacgcttt cgtcacggac atttgtgtca
     1621 tcataaaatt attcagatga cgacaaattc aaatggtgaa aaagcagttc ttgtcaccgg
     1681 ggggtccgga ttcatagcag tacattgcat cctccaacta ctaaacgccg gataccgcgt
     1741 aaaaacaacc gtacggtcgc tgacccgtga acccgaagtc agggccatgc taaaggtggg
     1801 tgggatcgag gctggcgaac acctgtcttt tatcgaagcg gatcttggct cggataccaa
     1861 ctgggacaaa gcggccggcg gctgcaccta tatcctgcac gtggcctcac cgacgcccct
     1921 gaaggcatac aaacacgaag acgaaatgat catacccgcc cgcgaaggag tcttgcgggt
     1981 actgcgttcc gctcgcaatg ccggtgtcag gcgggtcgtg ctgacatctg ccttcggcgc
     2041 cattgtctat ggccacaaac ctcaactgac gccatttacg gagaaggatt ggacaaatac
     2101 caccggcaag gttccccctt atcagaaatc aaaaacattg gccgaacgcg cggcctggga
     2161 tttcataggg aaagaaggcg gtcagctcga actttctgtt gtcaacccgg tgactgtctt
     2221 aggtccggta ttgggcccgg attattccca ttcgatccac ctcattaaaa acctgatcac
     2281 cggcagtatg cccggctgtc cgaagatcaa ttcagccttc gtcgatgtgc gcgacgtggc
     2341 ggatctgcac ctgcgggcaa tgacgcaccc tgccgccaac ggcgaacgtt ttatcgcca
//
LOCUS       DATGFG010000350        11366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1625725, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000350 DATGFG010000000
VERSION     DATGFG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625725"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VKQ52_06650"
     CDS             complement(<1..607)
                     /locus_tag="VKQ52_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLZ86896.1"
                     /translation="MNLVKVVALFCIGLLTTNGPASAQQALQPRKKSIGNETPEQKQQ
                     RMAWWTHDRFGMFIHWGLYALPARHEWVKRWERMNNDQYKIYFDLFNPDLFDPRSWAR
                     QAKAAGMKYAVLTTKHHEGFCLFDSKYTDYKATNTPAHRDLVREFVDAFRAEGIKIGF
                     YYSLLDWHHPDYIVDELHPLQPANNADTAYARLNKGRDWSRY"
     gene            complement(618..3905)
                     /locus_tag="VKQ52_06655"
     CDS             complement(618..3905)
                     /locus_tag="VKQ52_06655"
                     /inference="COORDINATES: protein motif:HMM:NF016945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HLZ86897.1"
                     /translation="MITYNKKPMSRKILFIFLLAAGLSGAAQTDPSFKWNHLPVKALL
                     LSCPDPKDVPLLCRFITDALPKEGVNTLCLRIEYQYQFESHPELADAGALSKQQLQSI
                     VAACRAAHIRFIPTMNLLAHQSEKTTIGPLLKHYPQFDESPDYNPPVPWKDGGMWDFY
                     TKCLCPQHPDLFKVLFPIMDELIDVCGADAMHVGLDEAWIIAYNKCPRCGGGDRSQIF
                     ADYVRKLHDHLRQKQCEMWMWSDRLIDGKTTNLLGWQASMNDTWRAIDMIPKDIMICD
                     WKYEDAPPTPGYFTIKGFNVLASACSKPDVALAQLDQIRLARKNGTREDFSLTLARRM
                     QGVFETMWFNTGDFIRAYDKPSGNSLIDNNVNTFKRLFAAIRQDEHPDAPAKSAAPET
                     AAPTNVTSETAAPTNPAPETPAPANATIDFSGPRQLAARCVPWLAPHLVFSRLDTNTG
                     KDAFRLSTMAGKVRIAATSPNAAAVGLGWYLKYYCQRSMSHMGDNLGPVAPLPAVAEP
                     LFRDCNAAIRYSLNYCTYNYSYSFYTWKDWEHELDWMALNGVNTMLVANGEEAVWQNV
                     LRQLGYSGKEIESFIPGPAYNAWWLMGNLQGWGGPMPLSQIDHRKRLVQKMLGRMHAL
                     GIQPVMPAFFGMVPSTLRNRLNAHIIPQGNWGAFVRPDILDPTDSAFDHIGSLFYAAT
                     KALYGDDIHYFSGDPFHEGGITDGVDLAAAGRKIQQLMQRFFPGSTWVLQGWQQNPRS
                     EMLAGVDRSKILVQELFGETTNNWEKRNAYEGSPFVWCMITNYGERPGLFGKLQRYAD
                     EPYRAAGTPLGKYLHGIGIMPEGINNNPVAYDLMLEVPWHEERIDAADWIQRFVLYRY
                     GKYNADLAKAWQLFLETIYSSPEGYQEGPPENILCARPSLQPRSVSSWGTLRKNYAID
                     RYAEAVKLFAAAAPAFPSSDTYALDLINFTRQVIANTADTVFGHWVAAYKQKDLPAFD
                     AETRHFLALADTLESLLDTHPYYRLSTWERKALAAGHTSAEKANNWRNALMLVTYWGE
                     NNRQEDYLHEYAYKEWAGMLRPFYKKRWELYFAYLKQQLNGVSAQEPDFFAWERSWVS
                     NRLNSKD"
     gene            complement(3902..5878)
                     /locus_tag="VKQ52_06660"
     CDS             complement(3902..5878)
                     /locus_tag="VKQ52_06660"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HLZ86898.1"
                     /translation="MKNIVLSLFLFASAVAGAQTPFRIGSESFLLHGQPFLIRCGEMH
                     FARIPRAYWRHRLKMAKAMGLNTICAYLFWNLHEPAPGNFNWKDQADAAAFCRMAQQE
                     GLYVLLRPGPYSCAEWDFGGFPWWLLQKRPLALRSRDPWYLRQCSGYLHEVGRQLAPL
                     QIDRGGNILMVQVENEYGSYGNDTTYIAALRDMLRDAGFTVPLFTCDGPLQLRNDTRS
                     DIFSAVNFGSDPEGGLKALRAVRPTGPLMVSEYYPGWFDSWGGRHMTGPADKVADAIR
                     YMLDHRASFSIYMVHGGTTFGLWSGANADPYMPETTSYDYDAPISEQGRATTKYFALR
                     NLLKAYLRPGESLPDPPPPIPVQTIPPFSCVAVARVLPQLLRHLLCDTLLSMESPAIG
                     EGHGDILYETTLPPGDAATLDVGEVHDYALVYLGDSLLATLDRRHGEHLVKIPARSTP
                     STLRIFVEAMGRVNYGPKLPDWKGLSGTPTLSATAKLSATPPLSATPTLTATPKLSDT
                     PILAGTPILSATPVRGWRIYPFPLEEASPRLLYSARPSPSGPAPTGPASKSPAPTSPS
                     PTGPALYKANFSVRRKADTFLDLRAFHKGVVWVNGHCLGRYWNIGPTQTMYLPGAWLN
                     IGANKVVVMDLFPATSPQLQGLDAPILDQTSALK"
     gene            complement(5881..7419)
                     /locus_tag="VKQ52_06665"
     CDS             complement(5881..7419)
                     /locus_tag="VKQ52_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86899.1"
                     /translation="MIRTFLTLLMAGLLSCAKKSVAPAAAAAKAATWYVAATGSDSNP
                     GTLAAPLKTISYALNQAVPGDTVMVRGGTYSGALTITRSGLLGKAITIKAYPGERPVL
                     DGSTITVTGWQAIVSLSNVRYIVFDGLDVANLTSSIANTDPEGIFINGASHDITISNC
                     NIYNIKNTTTLADGRSGHAVLVIGSDNNTAISNLMITGCIVHDTRTGTSENITLAGNV
                     DSFTVSHNTIYNTENIGIIIAGGDNLNRSGNVTVNYARNGVVSDNTLYNVSMANSVST
                     WGAGNYGAIAIYICGGAGTVVERNTVYACDRGIGLVSESSIYATRTSIVRNNFVYNCW
                     RTGIYMGDYLNFTGSGTKSCYVVNNTLFGNDQAPGAFGEIEGELRLTEHCDSNVFMNN
                     LVYAGTADLFVHKYSTTGAHNVIDHNLYFSTGIPGWLWQSTNGTPSSTLAAWQTVSGL
                     DAASLYGVDPLLVSTATPDLHIQPASPAKNAGVPLPMAVSGSTDIDGNPRIVNGKISI
                     GAQQ"
     gene            complement(7416..8729)
                     /locus_tag="VKQ52_06670"
     CDS             complement(7416..8729)
                     /locus_tag="VKQ52_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HLZ86900.1"
                     /translation="MQRIIQLLLLPSTFFLLAALSTGCSKTNGFQHHTGSAMAVDSFI
                     PSKGGLGTEILISGLNYTTDTNQVAVIINGHQLKVVKVDGRQIMAVVPAKCGSGHIIV
                     KIGNDSVVSAGMFNYQYAHIVSTLAGKGTAGFANGNGTEAEFSFGGQNWYRSMGITVD
                     SNLNVYVADPGNHCIRKIDSSGNVTTLAGDPTNSGYADGKGAAARFSLPYDVALDPAG
                     NIWSVDPGNWDIRKIAPDGTAVTWAWGGQAPWSISIDPISGDPFYSSCANPGNVYQVT
                     AQWNSSAIISGLDYPAGIRFDRSGNLYVVGNGDNVVHKFSVSTWTDSVIAGNGTAGYV
                     DGPATQAEFSAPWGLGLDAAGNLYIAGNGTWDGGSYNPDQSIRYVDAGTRAVSTFAGS
                     GTSGYIDGVGAAAAFSAPTGVCVDKNGTVYILDKNNNRIRKIITQ"
     gene            complement(8760..10589)
                     /locus_tag="VKQ52_06675"
     CDS             complement(8760..10589)
                     /locus_tag="VKQ52_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ86901.1"
                     /translation="MKKPEYIILIVLTSLSLFSCKKYLDERPDNLLTEDQIWQTRANA
                     EAYMYNIYSLLHNSDGGDYAAMGAADESSVCIAGTNVRQMVSGNWNASSAYFYNWGSY
                     YAGIRETIVFEQNIGKVPSTQLSDSLKTQYTAEGRFLRGYFYWQLLRQYGPFVKVAGL
                     ISQTANDNVYPRSPFDSCVAYIDQLMDEAGAHLPIAWSVSTNYGRASRGACLAIKAQA
                     ALLAASPLWNGNPYFANFKNHDGTMLAPASADVNRWKTAAAAAKAVIDLDAHHLFTNL
                     DEGGAAFDPYLSVRDLFLVNWNSEIILANNQWSQWGYTKCVSPGPGGYNMYNATQNVV
                     DAFYMANGRTIDDPLSGYVETGFAAAADTNYWGHLRGEWNMYANREARFYAFIQYNMH
                     PVLPAPTVDDKNYYSSSANQDGTGRVELYYSGLSGQKAAGSNRITGYGVLKNVSPADN
                     IRLDHTNYRPQILIRYAEILLDYVEALNEYDPGNPDIVTYLDMIRARAGLRGIETTYP
                     SAVGDQNQMRSYILRERQIELCFENDRYFTLIRRKLLGNSVNQSIYSMNTGADDGGLG
                     FSFTGFYTRTLFQTRTWDDKMYLFPIEQSDIDNDNSIVQNPGW"
     gene            complement(10607..>11366)
                     /locus_tag="VKQ52_06680"
     CDS             complement(10607..>11366)
                     /locus_tag="VKQ52_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86902.1"
                     /translation="EDEPVRKYPWMRATGTKFNEFYGYTSLGLFQDQNDIAKSPVQTL
                     NTTIYPGDIKYKDLNGDGSVDAYDEKYLKRSSFPDWSYGTGFSLSYRKFDLSMLFQGV
                     AGVGIMANGSSVAINNAGAAGVGVIPFAGIGQYPANILNNVTSRWTIANPSQHVAYPR
                     LSIGASTTSNNYVNSTWWLKDGAYTRLKQASIGYTLSNAKLKTSGVGSLYFYLAGTNL
                     LTFSKFKLWDPELGSDGAGYPPVRTVVAGIRANF"
BASE COUNT         2259 a         2912 c         3632 g         2563 t
ORIGIN      
        1 gataacggct ccagtcccta cccttgttga gccgggcgta agcggtgtca gcgttattgg
       61 ccggctgcag cggatgaagc tcatctacaa tatagtcggg atgatgccag tccagtagcg
      121 aataatagaa cccgatcttt atcccttcag cacgaaaggc atcgacgaat tcccggacga
      181 ggtcacggtg agcgggagta ttggtagctt tgtaatccgt gtatttcgaa tcgaaaagac
      241 agaatccctc atggtgtttg gtcgtcagta cagcgtattt catgcccgcg gctttggcct
      301 ggcgggccca ggacctgggg tcgaagaggt cggggttgaa tagatcgaag tatatcttat
      361 actgatcgtt gttcatgcgc tcccaccgct taacccattc atgccgggcg ggaagggcat
      421 agagccccca gtggatgaac atgccgaagc ggtcgtgcgt ccaccaggcc atgcgctgct
      481 gtttttgttc cggcgtttcg ttgccgatgc tttttttccg aggctgcagc gcctgctggg
      541 ccgatgcagg cccgttggtg gtgaggagtc ctatacagaa cagtgctact acttttacga
      601 ggttcatggc ttacttttta atccttacta tttaatcggt tactaaccca ggaccgctcc
      661 caggcaaaaa agtccggttc ctgcgctgaa acgccgttga gctgctgttt gagataagca
      721 aaatacaatt cccagcgttt cttataaaag ggccgtagca tgcctgccca ttccttgtac
      781 gcatattcgt gaagatagtc ttcttgccgg ttattctctc cccaataggt gacgagcatc
      841 agcgcatttc gccaattgtt ggctttctcc gcggaggtgt gacctgcggc cagggctttc
      901 cgctcccagg tagaaaggcg gtaatagggg tgagtatcga gaaggctctc gagtgtatcg
      961 gccagggcga ggaaatggcg tgtttccgca tcgaaggcag gcaggtcctt ttgcttgtag
     1021 gcggccaccc agtggccgaa tacggtatcc gcggtattgg caatgacctg gcgggtaaag
     1081 ttgatcaggt caagcgcata ggtatcgctg cttgggaagg cgggcgccgc agcggcgaaa
     1141 agctttacgg cctccgcata acgatcgatg gcataattct tacgaagcgt tccccagctg
     1201 ctgacggatc tgggctgcag ggaggggcgg gcgcacagga tattctcggg tggcccctcc
     1261 tgatagcctt cggggctcga atagatggtt tccaggaaaa gttgccaggc ctttgcgagg
     1321 tctgcattat acttaccata ccggtagagt acaaaccgct gtatccaatc ggcggcatcg
     1381 atgcgctcct catgccaggg cacttccagc atcaggtcgt aggccaccgg attattgttt
     1441 atcccttccg gcatgatccc tatgccgtga agatactttc ccaggggggt gcccgcggcg
     1501 cggtaaggtt cgtcggcata ccgttggagc ttgccgaaga ggcccggtcg ttctccgtaa
     1561 ttggtgatca tgcaccagac aaaggggctg ccttcatagg cattgcgttt ttcccagttg
     1621 ttggtggtct cgccgaagag ttcctggaca agtattttcg aacggtcgac gccggcgagc
     1681 atttccgacc ggggattctg ctgccatccc tggagcaccc aggtagaacc ggggaaaaaa
     1741 cgctgcatga gctgctggat tttccggccg gcagccgcga ggtcaacgcc atctgtaatg
     1801 cctccctcgt gaaaagggtc gccggagaaa tagtggatat cgtcgccata gagcgctttg
     1861 gtcgccgcat agaagagact gccgatgtgg tcgaaggcgg agtctgtggg gtcgaggata
     1921 tccggccgga caaacgcgcc ccagttgccc tgggggatga tatgtgcgtt cagcctgttg
     1981 cgaagggtcg atgggaccat accgaagaaa gccggcatca cgggctgaat gcccagggcg
     2041 tgcatgcggc caagcatttt ttgcaccaga cgtttccggt ggtctatttg cgagagcggc
     2101 atggggccgc cccagccctg gaggttgccc atgagccacc aggcattgta ggcagggccg
     2161 gggataaagg attcgatctc ttttccggaa tagccgagct gccggagcac gttctgccat
     2221 acggcctctt cgccgttggc tacgagcatg gtattgacgc cgttcaaggc catccagtcc
     2281 agctcatgct cccaatcctt ccaggtgtag aagctatagc tatagttgta ggtgcaataa
     2341 ttgagggaat agcggatcgc ggcgttgcaa tctctaaaga gtggctccgc gactgccgga
     2401 agtggggcga caggtcccag gttgtcgccc atatggctca tggagcgctg acagtaatat
     2461 ttgagatacc atcccagccc tacggctgct gcgttggggc ttgttgcggc gatacggact
     2521 ttgccggcca tggtggagag tcgaaaggca tctttgcctg tgttagtgtc cagccgggag
     2581 aagaccagat gaggcgccag ccaggggaca caacgggccg caagctgccg cgggccggag
     2641 aagtcgatgg tggcgttggc tggggcgggc gtttcggggg ctgggttggt tggagcggcc
     2701 gtttccgagg tgacgttagt gggtgcggcg gtttccgggg cggcactttt tgcgggggcg
     2761 tcgggatgtt cgtcctgccg gatggctgca aacagccgtt tgaaggtgtt cacgttgttg
     2821 tcgatcaggc tgttgccgga gggcttatca taagcgcgga tgaagtcgcc cgtattgaac
     2881 cacatggtct cgaatacgcc ctgcatgcga cgggccaggg taagagagaa atcctcccgt
     2941 gttccgttct ttcgcgccag cctgatctga tcaagctggg ccaacgccac atccggtttg
     3001 gagcaggcgc tggccagcac gttgaaacct ttgatcgtga agtaaccggg tgtcgggggt
     3061 gcgtcctcat atttccagtc gcagatcatg atatccttcg ggatcatatc gatggcgcgc
     3121 caggtgtcgt tcatgctcgc ctgccagccc aggaggttgg tggtcttacc atcgatgagc
     3181 cggtcgctcc acatccacat ctcacactgt ttttgccgga gatggtcatg gagtttccgc
     3241 acatagtccg cgaagatctg cgaccgatcg ccgccgccac agcgcgggca tttattatag
     3301 gcgatgatcc aggcctcgtc gaggcccacg tgcatggcat ccgcgccgca gacatcgatc
     3361 agctcgtcca tgatgggaaa cagcaccttg aaaagatcgg gatgctgcgg gcagaggcac
     3421 ttggtataga aatcccacat accgccatct ttccagggta cgggcggatt gtaatcgggc
     3481 gattcgtcga attggggata atgctttagc aaggggccga tggtagtctt ttccgattgg
     3541 tgagccagga ggttcatggt aggtataaag cggatatgcg cagccctgca agctgccacg
     3601 atgctttgca actgctgttt ggacagggcg cccgcgtcgg cgagctcggg gtgcgactcg
     3661 aactgatact ggtactcgat acgcagacag agcgtgttta cgccctcttt gggcagggcg
     3721 tccgtgataa agcggcaaag caatgggacg tctttcgggt cggggcagct cagcagcagg
     3781 gcttttaccg gcagatggtt ccatttaaag gagggatcgg tctgtgcggc tccgctaaga
     3841 ccagcggcca gcaggaagat gaaaagaatt ttccgggaca tcggcttttt attgtaggtg
     3901 atcatttgag tgcggaggtt tggtccagga tcggtgcgtc gagtccctgc aattgcgggg
     3961 aggtggctgg aaagaggtcc atgaccacga ctttgttggc gcctatgttt agccaggcgc
     4021 cgggaagata catggtttgg gtgggtccta tgttccaata tcgtcccaaa caatgaccgt
     4081 tgacccagac aacccctttg tggaaggcgc gcaggtcgag aaaggtgtcg gcttttcggc
     4141 ggacgctgaa gttcgctttg tagagggcgg ggccggttgg cgaggggctg gttggtgcgg
     4201 ggctttttga tgcggggccg gttggcgcgg ggcccgatgg cgaggggcgt gctgaataga
     4261 gaaggcgggg acttgcttct tccagcggga aaggatatat acgccagccc ctcaccggcg
     4321 tggcggagag tatcggtgtg ccggcaagta tcggtgtgtc ggagagtttc ggcgtggcgg
     4381 tgagcgtcgg cgtggcggag agcggcgggg tggcgctgag tttcgccgtg gcgctgagcg
     4441 tcggcgtgcc ggagaggccc ttccaatcgg gcaatttggg cccgtagttg acgcggccca
     4501 tggcttcgac gaagatgcgc agagtcgaag gggtgctcct tgcgggaatt ttgacgaggt
     4561 gctcaccatg gcggcgatcg agggtggcta agaggctgtc gcccaggtat accagcgcat
     4621 aatcatggac ttctccaaca tcgagtgttg cggcgtctcc cggaggcaag gtggtctcat
     4681 agagaatatc gccatgtccc tcaccgatgg ccgggctttc catgctcagc aaggtatcgc
     4741 aaaggaggtg gcggagcaat tgggggagca cccgggccac ggcgacgcag gaaaaaggcg
     4801 ggatggtttg caccggtatc ggcggcgggg gatcgggcag gctttcgccg ggccgcagat
     4861 atgctttcaa caagttgcgg agggcaaagt atttggttgt cgcccgacct tgttcgctaa
     4921 tgggcgcatc atagtcatag gaagtcgtct cgggcatata tggatcggcg ttagcgcccg
     4981 accataatcc gaaggtggtg ccgccgtgga ccatataaat gctgaaggaa gcccggtgat
     5041 cgagcatgta gcggatagcg tccgcgactt tatccgcggg gccggtcatg tgcctgccgc
     5101 cccagctgtc gaaccagccg ggataatatt cgctgaccat cagcggcccc gtggggcgga
     5161 cggcgcgcag ggctttcagg cctccctccg gatcgctgcc gaagttgacg gcggagaaga
     5221 tatcgctgcg cgtatcgttg cgcaattgca ggggtccatc gcaggtgaag agcggaacgg
     5281 tgaagcccgc gtcacgcagc atatccctaa gcgcggcaat gtaggttgta tcgttgccat
     5341 agctgccgta ttcgttctcc acctggacca taagaatatt tccgccccgg tcgatctgca
     5401 agggggccag ttgccggcct acctcgtgga gatacccgct gcattgccgc agataccagg
     5461 gatcgcggga gcggagggca aggggccttt tttgcaggag ccaccagggg aagcccccga
     5521 aatcccactc ggcgcacgaa taggggccgg ggcggagcag gacatacagc ccttcctgct
     5581 gtgccatccg gcaaaaggca gcggcatctg cctggtcctt ccagttgaaa ttaccgggag
     5641 ccggctcgtg caggttccaa aagagatagg cgcagatggt attaagcccc atcgccttcg
     5701 ccatcttcaa gcgatgccgc caatacgcgc ggggtatccg ggcaaaatgc atctcgccgc
     5761 aacggatcag gaacggctgt ccatggagga gaaagctctc actgccgatg cgaaaggggg
     5821 tttgggcgcc ggctaccgca gaggcgaaga ggaaaaggga tagtacgatg ttcttcatga
     5881 ctattgctgg gcgccgatgc ttatttttcc atttacgata cgggggttgc catcgatgtc
     5941 ggtgctgccg ctgacagcca tcgggagggg gacgccggca ttctttgccg gtgaagccgg
     6001 ttgaatatgc aggtccggcg tcgccgtgct cacaagcagg ggatcgacac catacaggga
     6061 agcggcatcc agtcccgaga cggtctgcca ggcggcaagt gtagaggacg gtgtgccgtt
     6121 ggtgctttgc caaagccagc ccggtatgcc cgtagaaaaa tacaggttgt gatcgatcac
     6181 gttatgtgcc cccgtggtgg agtacttgtg tacaaagaga tcggcggtac cggcatagac
     6241 caggttgttc ataaagacat tgctgtcaca atgttcggtg agccgtaatt ccccttcgat
     6301 ctcgccgaag gcaccagggg cctggtcgtt gccgaagagg gtattgttca ccacatagca
     6361 gctcttcgtg ccgctgccgg tgaagttcag gtaatcgccc atatagatgc ccgtgcgcca
     6421 gcagttgtag acaaagttgt tcctgacgat ggaagtgcgg gtcgcataga tgctgctctc
     6481 actgaccaga ccgatgccgc ggtcgcaggc atagacggtg ttgcgttcga ccactgttcc
     6541 ggcgccgccg cagatataga tggcgatggc gccgtagttg cccgcgcccc aggtggacac
     6601 gctgttggcc atggagacat tatacagggt attgtcgctg acaacaccgt tgcgggcgta
     6661 attgacggtt acatttcccg accggttgag gttatcaccg ccggcgatga tgatgccgat
     6721 attctcggta ttgtagatgg tattatggct cacggtaaag ctgtccacgt tgccggccag
     6781 ggtgatgttc tcgctggtcc ccgtccgggt gtcatggacg atgcagccgg tgatcataag
     6841 attggaaatg gcggtgttgt tatcgctgcc gatgactaag acggcatgac cgctgcgccc
     6901 gtcggccagg gtggtcgtat tttttatgtt atagatattg caattgctga tggtaatgtc
     6961 atgtgaagcg ccgttgataa aaataccttc ggggtctgta ttggcgatgc tgctggtgag
     7021 attggccaca tcgagtccat cgaaaacgat gtaccggaca ttgctgaggc tgacgatggc
     7081 ctgccatccg gtgaccgtga tggtgctgcc atcgagaacg ggtctttcgc ccggataggc
     7141 tttaatagta atggctttgc ccagcaatcc cgagcgggta atggtgagcg caccgctata
     7201 ggtgccgccg cggaccatga cggtgtcgcc gggcacggcc tggttcaatg cataggaaat
     7261 ggtcttgagc ggagcggcca gggtgcccgg attggaatcg ctgcccgtgg cagcaacgta
     7321 ccaggtggca gctttggctg ccgcagcagc aggagcaacg ctcttttttg cgcaggagag
     7381 aagaccggcc atcagcagcg taaggaatgt cctgatcatt gggtaattat ttttctgatc
     7441 ctgttgttgt tcttatccag tatgtaaacg gttccgttct tatccacgca tacgccggtg
     7501 ggggcgctga aggcggcggc tgcgcctaca ccgtcgatat accccgaggt cccgctgccg
     7561 gcgaaggtgc ttacggcgcg ggtacccgca tcgacatacc ggatggactg atcgggatta
     7621 taggagccgc cgtcccaggt tccgttgccc gctatataga ggttgccggc ggcatcgagg
     7681 ccaaggcccc agggcgcgga aaactccgcc tgtgtggcgg ggccatccac atagccggcc
     7741 gtcccatttc cggcgatgac gctgtctgtc caggtgctta cggaaaattt atggaccaca
     7801 ttgtcgccgt tgcccacaac atagagattg cctgagcggt cgaaccgtat gcccgcggga
     7861 tagtcgagac cggatatgat cgcgctgctg ttccattgtg ccgtcacctg atagacattc
     7921 ccgggattgg cgcagctgct gtagaaagga tcgccgctga tggggtcgat ggaaatgctc
     7981 cagggcgcct ggccgcccca ggcccacgtg acggccgtac catcgggagc gatcttccgg
     8041 atatcccagt tgcccggatc tacggaccag atgttgcctg cggggtccag cgctacatca
     8101 taagggaggc tgaacctggc ggcggcgcct ttaccatcgg cataaccgct gtttgtgggg
     8161 tcacccgcca gggtggtcac attgccgctg ctgtcgatct ttctgatgca atggttgccg
     8221 ggatcggcca catagacatt caggttacta tccaccgtga tgcccatgct ccggtaccag
     8281 ttctgcccgc cgaaactgaa ttccgcctcg gtgccgttac cgttggcgaa gccggccgtg
     8341 cctttgccgg ccagcgtgct cacgatatgg gcatactggt aattgaacat gccggcgctc
     8401 accacactgt cgttgcctat tttcactata atgtgccctg atccgcattt ggcgggaacg
     8461 acggccatga tctgccggcc gtcgactttg accactttaa gctgatggcc gttgatgata
     8521 acggccacct gattggtatc ggtagtatag ttgaggccgc tgatgagaat ctccgttccc
     8581 aggccgccct tggacgggat aaaactgtcg acggccatgg cggaaccggt gtggtgctga
     8641 aaaccattgg ttttgctaca gccggtggaa agggcggcca ggaggaagaa agtggatggt
     8701 agcaggagta attgtatgat gcgttgcatt gggttgttgt ttggatgaat gaggatcgtt
     8761 taccagcccg ggttttgtac aatggaattg tcattatcga tgtccgactg ttcgatcggg
     8821 aagaggtaca tcttatcgtc ccatgtccgc gtctgaaaaa gcgtgcgggt atagaagccg
     8881 gtgaaggaaa aacccagtcc gccgtcatcc gcgcctgtat tcatactata gatagactgg
     8941 ttgacggagt tacccagcag ctttcgccgg atcagcgtaa agtatcggtc attctcgaag
     9001 cagagctcaa tttgccgttc gcgcaatata tagctgcgca tctggttttg atcgcctacg
     9061 gcggagggat aggtggtctc gatgccccgc agccctgccc tggccctgat catatcgagg
     9121 taggtaacga tatcggggtt acccgggtcg tattcgttga gggcttctac ataatccagg
     9181 aggatctcgg catagcggat gaggatctgt ggcctgtaat tggtatggtc cagccggata
     9241 ttgtccgcgg gactgacatt cttgagcacg ccatagcccg tgatcctgtt gctgcccgcg
     9301 gctttttgtc ccgagaggcc gctgtagtag agctctaccc tgccggtgcc gtcctggttg
     9361 gcggaggagc tgtagtagtt tttatcgtca acggtgggag ccggaagcac gggatgcata
     9421 ttgtattgga taaaggcata gaaccgggcc tcgcggttgg catacatatt ccattcaccc
     9481 cggaggtgac cccagtagtt ggtgtccgcc gctgcggcga atccggtttc cacgtagccg
     9541 gagaggggat cgtcgatggt gcggccattg gccatataaa aagcgtccac gacgttctgg
     9601 gtggcattat acatgttgta gccgccgggg cccggagaca cgcatttcgt atatccccat
     9661 tgcgaccatt ggttgttggc gaggatgatc tcgctgttcc agttgaccag gaacaggtcg
     9721 cggacggata gataggggtc gaatgcagcg ccgccctcgt cgaggttggt aaacaggtga
     9781 tgggcgtcaa ggtcgatgac agcctttgcc gcggccgcgg cggtcttcca gcggttgaca
     9841 tccgccgatg cgggcgccag cattgtgccg tcgtgattct tgaaattagc gaaataggga
     9901 ttgccgttcc acagcgggct ggcggcgagc agggctgcct gggcttttat ggcgagacag
     9961 gctccccggc tggcgcggcc gtagttggtg ctgacggacc atgcgatggg aaggtgggcg
    10021 ccggcctcgt ccatcagttg gtcgatatag gcgacgcagc tatcgaaggg ggaacgggga
    10081 tagacattgt cattggccgt ctggctgatg agaccggcga cctttacgaa gggaccgtat
    10141 tgccgcagca gctgccagta gaaatagccg cggaggaacc ggccctctgc cgtatactgt
    10201 gtcttgaggc tgtcgctaag ctgggtagaa gggaccttcc cgatattctg ttcgaagacg
    10261 atcgtttccc ggatgccggc atagtagctg ccccagttgt aaaaataggc cgaggaggcg
    10321 ttccagttgc cgctgaccat ttgccggacg ttggtgccgg cgatgcatac cgagctttca
    10381 tcggccgcgc ccatggctgc atagtcgccg ccgtcggaat tatgcagcag gctatagata
    10441 ttgtacatat aggcttccgc attggccctg gtctgccaga tctggtcttc ggtgagcagg
    10501 ttgtcgggcc gctcgtccag gtatttcttg caggaaaaaa gggacagcga ggtcagtacg
    10561 atgagtatta tatattcagg ctttttcatt tccggtgttt taagcattaa aaattggccc
    10621 ttatcccggc tacgacggtg cggacagggg gataaccggc tccgtcggaa cccagctcgg
    10681 ggtcccataa tttgaatttg gaaaaagtga gcaggttggt gccggcgagg taaaaataga
    10741 ggctgccgac gccggatgtt ttaagctttg cgttggacaa ggtatagccg atggacgcct
    10801 gtttaagccg ggtataggcg ccgtctttca accaccaggt gctattgaca taattgttgg
    10861 aggtggtgga agcgccgatg ctcaggcgtg gataggcgac gtgctggctg gggttggcga
    10921 tggtccagcg gctggtgaca ttgttcagga tattggcggg atactggccg atgccggcga
    10981 agggaatgac gccgacgccc gcggctccgg cgttgttgat ggcgaccgat gagccgttgg
    11041 ccatgatgcc gacgccggca acaccctgga agagcatgga gaggtcgaat ttcctatagg
    11101 aaagcgagaa acctgttcca tacgaccagt cggggaagga ggatctcttc aggtatttct
    11161 cgtcataggc atcgaccgac ccatccccat tgaggtcttt atatttgatg tcccccgggt
    11221 agatggtggt attcagcgtc tgcacgggac tcttggcgat gtcgttctgg tcctggaaaa
    11281 ggcccaggga agtatagcca tagaactcgt tgaattttgt accggtagcc cgcatccagg
    11341 ggtatttacg gacgggctcg tcctcg
//
LOCUS       DATGFG010000351         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1053831, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000351 DATGFG010000000
VERSION     DATGFG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053831"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VKQ52_06685"
     CDS             <1..373
                     /locus_tag="VKQ52_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HLZ86903.1"
                     /translation="ALLGAMSRRGARRVFFISASAVEISPLLPALVRFAAKYILQKLL
                     RHMYADLRRMERRVKESDLDWTIVRPPRLTDKPMTGRYRFAVNSYLRNCLSISRADVA
                     HFMIHHAEDRGTFRAVVEVGY"
     gene            601..780
                     /locus_tag="VKQ52_06690"
     CDS             601..780
                     /locus_tag="VKQ52_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86904.1"
                     /translation="MHQKKQKKKGRPRKAGRRWKSIYATLRDITGIIGALGTFVQALP
                     YLGKLILELWRFFGL"
     gene            complement(837..2150)
                     /locus_tag="VKQ52_06695"
     CDS             complement(837..2150)
                     /locus_tag="VKQ52_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLZ86905.1"
                     /translation="MEPRSVHTTCCVVGGGPAGMITGFLLARAGIPVVVLEKHRDFFR
                     DFRGDTLHPSTFNIMAQLGLLDAFLKVPHQEITELSAVYNGRRLRFADFSHLHAAKPA
                     LGLMPQWDFLNFITEQASKYPNFTLLRETNVTGLLQTPTAPPLSNTPAAPGTQSPPTN
                     PHLQPPRITGVHAETPAGGLDIHASLVIGADGRGSTVRKAAGLEPIETGVPIDVLWFR
                     LSRHPDDPGTVFGNFYRGRILILVYRGDYWQCGLVIDKGGFEKIKADGLENFRDQLTV
                     ICPFLKNRIHELTDWSKVNLLVVKIDHLETWYTNGLLCIGDAAHAMSPVGGVGINLAI
                     QDAVATANILWAPLKNNQPITETLLRKVQKRRAWPTRITQAFQATVHKGISYKREGTA
                     EKGPPLFFLLLHHVALLRRLPARFLGQGVRPETVRCPEAPPAPGT"
     gene            2180..2533
                     /locus_tag="VKQ52_06700"
     CDS             2180..2533
                     /locus_tag="VKQ52_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86906.1"
                     /translation="MKPLIDLSHWKVVRELMDELLFPGTEESTSYGTPAFKVKKKLLL
                     RLKEDGESLVIHSDDRDVWLAEDPTVFFITDHYRDYPYVLVRLKKIGKKKLRMLLVQS
                     WKELAPQKLVEEWES"
     gene            complement(2609..>2987)
                     /locus_tag="VKQ52_06705"
     CDS             complement(2609..>2987)
                     /locus_tag="VKQ52_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86907.1"
                     /translation="EDSSLSYPLRAYYIDSVLNKDIHFPDTIKSKFYISDVQTDLHEG
                     NRLIHFKERPEEWYLVELLGGGQSVIDAIYNPEISDHAIFDKKNLREWQLTRITNRMQ
                     TEILNKMELYARKNHGLDSTEKK"
BASE COUNT          627 a          664 c          951 g          745 t
ORIGIN      
        1 tgctttgctc ggggcgatga gccggcgggg cgccaggcgg gtcttcttta tctcggcttc
       61 ggctgtggag atcagtccgt tgctgccggc gctggtgcgt tttgccgcga aatatatcct
      121 gcagaaattg ttgcggcata tgtatgccga tctgcgccgg atggagcgcc gggtgaagga
      181 gagcgatctc gactggacga tcgtccggcc gccgaggctg acagataagc ccatgacggg
      241 acgttaccgg tttgcggtga acagttacct gcgtaattgt ctcagtatat cgcgggcgga
      301 tgtcgcgcat tttatgatcc accatgcgga ggacaggggg acgttcaggg ctgtggtgga
      361 ggtgggttat tgaattggct caaggtattt gttaaatggg ggcggaagat gggtttcttc
      421 cgtccttttt cgtatctttt ataggtccga tagttctttg actgatggcc ccggttcaac
      481 ttctgccgga tgtcttcttt tcgcgccgtg gcctgtgctg cggcagtcaa cctcgacggg
      541 cgaaagccga aagcccgata ggctacaagc caaagcccga tactctatag gagtacttac
      601 atgcatcaaa aaaagcagaa gaaaaaaggc cgtccccgta aggccggtcg ccgttggaaa
      661 tccatctatg ccacgctcag ggacatcacc gggataatcg gggccttagg caccttcgtg
      721 caggccctgc cctatctcgg aaagctgatc ctggagttgt ggcgcttctt cggtctttga
      781 tcaccgtact ttatcgacag ccccgggcca tggggctgtt tttttttgag gtgacctcac
      841 gtgcccgggg cgggcggtgc ttcggggcaa cgcacggttt cggggcggac gccctggccg
      901 aggaagcggg cggggagtcg tcgcaggagg gcgacgtggt gaagcaggag gaagaagagg
      961 ggtggtcctt tttctgctgt accttctctt ttatagctga tgcctttgtg gacggtggcc
     1021 tggaatgcct gggtgatccg ggtgggccag gcccggcgtt tctggacctt tctcagcagg
     1081 gtttccgtta tgggttggtt gttcttcaac ggcgcccaga ggatattggc ggtggcgacg
     1141 gcgtcctgga tcgcgaggtt gatccctacc ccgccgacgg gtgacatggc gtgcgcggcg
     1201 tcgccgatgc agaggaggcc gttggtgtac caggtctcga gatgatcgat ctttactacg
     1261 aggaggttga cctttgacca gtcggtgagt tcgtggatac gatttttgag aaaggggcag
     1321 atgaccgtga gctgatcgcg aaaattttcg aggccgtccg cctttatttt ttcgaagccg
     1381 cctttgtcga tgacgaggcc gcattgccag tagtcgccgc ggtagacgag gatgaggatg
     1441 cggccacggt agaagttgcc gaagacggtt ccggggtcgt cggggtggcg ggagagccgg
     1501 aaccagagaa catcgatggg gacgccggtt tcgatgggtt cgaggcccgc ggccttgcgg
     1561 acggtggagc cacgaccgtc ggcgccgatg acgagagagg catggatgtc aagccctccg
     1621 gcgggggttt cggcgtggac gccggtgatg cggggcggct ggagatgggg gttagtggga
     1681 ggtgattggg tgccgggagc cgccggggtg ttggagagtg gtggagcggt gggtgtttgc
     1741 aggagcccgg tgacgtttgt ttcgcggagg agggtgaagt tgggatattt tgacgcctgt
     1801 tcggtaatga aattcaggaa gtcccattgc ggcatgagcc cgagcgcggg ctttgcggcg
     1861 tgtaaatggg aaaagtcggc gaagcgcagc cggcgaccat tgtagacggc ggatagctcg
     1921 gttatctcct ggtggggcac ttttaaaaat gcatccagga ggcccagttg cgccatgatg
     1981 ttgaaggtag agggatggag ggtatcgccg cggaagtcgc gaaagaaatc gcgatgtttt
     2041 tcgaggacca cgacgggtat gccggccctt gcgaggagga agccggtgat cattccggcc
     2101 gggccgccac ccacgacgca gcaggttgta tggacggacc tcggttccat agcataaaat
     2161 taatggatat ctttgacaga tgaagcccct gatcgacctc agccattgga aagttgtccg
     2221 ggaattgatg gatgagttgc tcttccccgg aaccgaggaa tcgaccagtt atgggacacc
     2281 tgcattcaag gtaaaaaaga aattacttct tcgcctgaaa gaggacgggg aaagcctggt
     2341 gatccattcg gatgaccggg atgtatggct ggctgaggat cccaccgttt tctttattac
     2401 ggaccactat cgcgactatc cctatgtgtt ggtgcggctt aaaaagatcg ggaagaaaaa
     2461 attaaggatg ttattggtgc agtcctggaa ggagttggct ccgcaaaagc ttgtagaaga
     2521 gtgggagagt tagtcgttgg cggggtctgt aaaatgaact gtgtcaggaa taattgatca
     2581 aattgaaata gatattttta aaatatcatt atttcttttc cgttgaatcg agaccatggt
     2641 tctttcgcgc atacaattcc atcttattta atatttcggt ttgcattcta tttgtgatcc
     2701 tcgtaagctg ccattccctt aaattctttt tgtcaaaaat ggcatgatcg gaaatttctg
     2761 ggttatatat tgcatcgatt acagattgac ctcctcccag caattctaca agataccact
     2821 cttcaggcct ctctttaaag tggatcaatc tgtttccctc atgtaagtca gtctgaacgt
     2881 cgcttatata aaattttgat ttaatagtat cagggaaatg aatatcctta tttaaaactg
     2941 aatcaatgta gtaagctctt aagggataac tgaggctgga atcttca
//
LOCUS       DATGFG010000352         4299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1398741, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000352 DATGFG010000000
VERSION     DATGFG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398741"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VKQ52_06710"
     CDS             complement(<1..1071)
                     /locus_tag="VKQ52_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86908.1"
                     /translation="MRGIFIGFGLFLLAVVVGIAYWYKGNHKAQLTIKGIVGSEKSNF
                     LENPRVRSILQSKYGLTLDYTRQGSIEMVSGPAREDIDFLWPSSQLALELFKTTQDAR
                     LLKSELVFNSPIVLYSWDIVVKALAKQGIIKEEDSVCYLTDMDRLIRWELSGQSWSGI
                     GLPDLYGKVSVTTTDPTKSNSGNLYAGLMANLLSRDGVNDPDSLERILPDLQKLFTRQ
                     GFMQSSSGFLFEQFLQTGVGQYPIIAGYENQIVEFSLQHPDFWPKVRDKIKIIYPVPT
                     VWSEHPVLILTKKALPLLDALKDPEIQRIAWEEHGFRTGLIGVQNDPKVIKVDGLARS
                     INKTMPMPRAVIMQKIIEALNTS"
     gene            complement(1077..1697)
                     /locus_tag="VKQ52_06715"
     CDS             complement(1077..1697)
                     /locus_tag="VKQ52_06715"
                     /inference="COORDINATES: protein motif:HMM:NF021617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HLZ86909.1"
                     /translation="MRTFTNIAGVLSGAIGGGGFLILFFLIQLPLLPSLLLALAGFGG
                     TYLFIYAFRPRPRIEFDIGAGITPQLLETTMKEGGEKVKALVDCAGDIKDPIIRAKIA
                     RIIDVIKRIFANFEKDPKDIKQAKQFLSYYLDTSIKIVRKYRDLSAQNVRSPEIAATL
                     SRAELMLDSIEKAFEKQHARLLRDDAMDLDTEIQTLESTFNAEDLK"
     gene            complement(1697..3283)
                     /locus_tag="VKQ52_06720"
     CDS             complement(1697..3283)
                     /locus_tag="VKQ52_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLZ86910.1"
                     /translation="MASADFRPPVRRPGIIIVALLLFTACQNHKSFRLLSGSENEALQ
                     PLIKEFAEKQGYDIDFAYKGSVDIMLELESDSTGFDAVWPASGLWITLGDQHHKVKYA
                     RSILTSPVVFGIKKSLAQKLNFIGHPVKVADILTAIRDKQLKFMMTSASQSNSGASAY
                     FGFLYALLGNPEFITINDLHKPELKDQIRQLLSGINRSSGSSGWLKDLFLQGNGQYDA
                     MVNYESTIVETNQELLRRNQEPLYVIYPTDGLVIADSQLGYVDQGDKDKEAFFRKLQD
                     YLLSAEVQTKLLQLGRRTGFAGELSNAPTDVFRADWGIDTKKILSPIKLPGEDVIREA
                     LSLYQTEFRKPSLTVFCLDFSASMNGNGGADQVKVAMQLLLDQQQARRYFLQASADDR
                     LIIIPFNHDLMPGWKVDGNQDDSLKAVLQQIQALYPGGGTDIYSPAIKGLEEIKQIDT
                     AGQYIPAVILMTDGESNTGKKFEDLQAAWQGIDIPVFAITFGDASDDQLKQITGLTRG
                     YLFDGKKDLVTAFRKAKGYN"
     gene            complement(3376..>4299)
                     /locus_tag="VKQ52_06725"
     CDS             complement(3376..>4299)
                     /locus_tag="VKQ52_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HLZ86911.1"
                     /translation="SLLLGVAVFVPFILLFGLAFLHPAAATTITTATTAGSNTIHGGY
                     GFHSVSLALYTVMWNFIGWDNASTYAGEVARPVRSYILSICIAFLLVIGIYTFSALTV
                     ARSGVDHQALLNGGFPVLGELIGGHWLGMLIAAGGMASTLGLYSAVLLSVSRVPQVMA
                     DDQLLPTRLCSLHPRFNTPYISIIASSIIVSILVLFTFSDLIVMDIILYGAGLSLEFI
                     ALLLLRIKEPDRPRPFRIPLGRIGLALMILLPIGIYTFALTGVIYTSRTGSWSSPQNM
                     TIPVLLALAMLFSAAPIWWLIRWRKPHLSTP"
BASE COUNT          853 a          970 c         1516 g          960 t
ORIGIN      
        1 acttgtattg agagcttcga ttatcttctg catgatgacg gcgcggggca tgggcatcgt
       61 tttgttgatg ctccgggcga ggccgtctac ttttatgacc ttggggtcgt tttgcacgcc
      121 gatgaggccg gtgcggaagc cgtgttcttc ccaggcgatg cgctggatct cggggtcctt
      181 caacgcgtcg aggaggggca gggctttttt ggtcaggatg agtacggggt gttcactcca
      241 gaccgtcgga acggggtaga tgatctttat cttatccctg acctttggcc agaagtcggg
      301 gtgctggagc gagaattcga caatctggtt ctcgtaaccc gcgatgatcg ggtattggcc
      361 cacgcctgtt tgcaggaatt gttcgaagag gaagccggag gagctttgca tgaagccctg
      421 acgggtgaag agcttttgca gatcggggag gatccgctcg aggctgtcgg ggtcattgac
      481 gccgtcgcgg ctgaggaggt tggccatgag gccggcgtag aggttaccgc tgttagactt
      541 tgtcggatcg gtggtagtga cgctcacctt gccatagaga tcggggaggc cgatgccaga
      601 ccaggactgg ccggagagtt cccagcgaat gagccggtcc atatccgtca gatagcagac
      661 gctgtcctct tcctttataa tgccctgttt ggcgagggcc tttacaacga tgtcccagct
      721 atagagaacg atgggtgagt tgaagacgag ttcggatttg aggaggcggg cgtcctgggt
      781 ggtcttgaag agttcgagcg cgagctggct ggagggccag aggaagtcga tgtcttccct
      841 ggcaggcccg ctcaccattt cgatggagcc ctggcgggta tagtcgaggg taaggccgta
      901 tttggactgg aggatggagc ggacgcgcgg gttttcgaga aagttgctct tttcgctgcc
      961 gacgataccc ttgatggtga gctgggcttt atggttgccc ttgtaccaat aggctatccc
     1021 gacgacgacc gccaatagga agagtccgaa tccgatgaat attccgcgca tggtgtttat
     1081 tttaggtcct ccgcgttgaa ggtgctttcc agggtctgga tctccgtgtc gagatccatc
     1141 gcgtcatcgc gcagcaggcg ggcgtgttgt ttttcaaacg ccttttcgat ggagtcgagc
     1201 atgagttcgg ccctggacag ggtggcggcg atctccgggg aacgaacgtt ctgtgcggag
     1261 aggtcgcggt atttgcggac gatctttata ctggtgtcga gataatagga caggaattgc
     1321 tttgcctgct tgatgtcttt cgggtccttt tcaaaattcg caaaaatgcg tttgatgaca
     1381 tcgatgatcc gggcgatctt ggcgcggatg atcgggtcct ttatatcgcc ggcgcagtcg
     1441 acgagggcct ttaccttttc gccgccttcc ttcatcgtgg tctcgagcag ctggggcgtg
     1501 atgccggcgc cgatatcgaa ttcgatgcgg ggtcttggtc ggaaggcgta gataaagaga
     1561 tatgttccac cgaagcccgc gagagcgaga agaagagagg gcagcaaggg tagctggatc
     1621 aggaagaaca ggatcagaaa tcccccgccg ccgatggcgc cggagagaac acctgctatg
     1681 ttggtgaagg ttcgcactag ttatatcctt ttgcttttct gaatgcagtg acgaggtctt
     1741 ttttgccgtc gaagagatac ccgcgggtaa gcccggtgat ctgctttagc tggtcgtcgg
     1801 atgcgtcgcc aaaggtgatc gcaaagacgg ggatgtcgat cccctgccag gcggcctgga
     1861 ggtcttcgaa ttttttcccg gtgttggatt cgccgtcggt catcaggatc accgcgggaa
     1921 tatattggcc ggccgtgtcg atctgtttta tctcttcgag cccttttatc gcggggctgt
     1981 agatatcggt gccgccgccg ggatagaggg cctggatctg ctgcaggacc gccttgaggg
     2041 aatcgtcctg gttgccatcg accttccatc cgggcatgag gtcgtggttg aaggggatga
     2101 tgatcagccg gtcgtcggca gaggcctgga ggaaatagcg tcttgcctgc tgctggtcga
     2161 ggaggagctg catggctacc ttcacctgat cggcgccacc attcccgttc atactggcgg
     2221 agaagtcgag acagaagaca gtgagcgagg gcttacgaaa ttccgtttgg tagaggctca
     2281 acgcttcgcg gatgacgtct tcgccgggaa gttttatggg agagaggatc ttctttgtgt
     2341 cgatgcccca gtcggcgcgg aagacatcgg tgggggcgtt ggagagttcg cccgcgaaac
     2401 cggtgcggcg acccagttgc aggagttttg tttgtacttc cgcgctgagg agatagtcct
     2461 ggagcttgcg gaagaaggct tccttgtcct tgtcgccctg gtcgacatag cccaattggg
     2521 agtcggcgat gacgaggccg tcggtgggat aaataacata cagcggctcc tggttgcggc
     2581 ggaggagttc ctggttggtc tcgacaatgg tggactcgta gttcaccatg gcgtcatact
     2641 gaccattgcc ctggaggaag aggtctttta gccagcccga gctgccggag gagcggttga
     2701 tgccggagag gagttgccgg atctggtctt ttagttcggg cttgtgaaga tcgttgatgg
     2761 tgatgaactc ggggttgccg aggagggcgt agaggaaccc gaagtaggcg ctggcacccg
     2821 agttggactg ggaggcggaa gtcatcatga acttcagctg tttgtcccgg atagcggtga
     2881 ggatgtcggc gacctttacg gggtggccga tgaagttcag tttttgggcc aggcttttct
     2941 ttatgccgaa gacgacgggg ctggtgagaa tcgatcttgc gtacttgacc ttgtggtgtt
     3001 ggtcgccgag ggtgatccag aggccgctgg caggccagac ggcgtcgaag ccggtgctgt
     3061 cggattcgag ttcgagcatg atatcgacgg agcctttgta ggcaaagtcg atgtcatagc
     3121 cttgtttttc ggcaaattcc tttatcaggg gctggagggc ttcgttctcc gagccggaga
     3181 ggaggcggaa ggacttatgg ttttggcagg cggtgaagag gaggagggca acaatgatga
     3241 tgccaggtcg ccggacgggt ggtctaaagt cagcagaagc caagggttgc ttttcgacaa
     3301 gatacgccgg gttatgatgg taaccgaatg agaccgcgcc gtagaacgaa gtttacaatt
     3361 atttaatata cgattttatg gcgtcgaaag gtgtggcttg cgccagcgga tcagccacca
     3421 gatgggtgcg gcggagaaga gcatcgcgag ggcgaggagg acggggatgg tcatgttttg
     3481 gggagatgac catgatcccg ttctgctggt atagatgacg ccggtgaggg cgaaggtgta
     3541 gatgccgatg ggcaggagga tcatgagggc caggccgatc ctgccgaggg ggatcctgaa
     3601 gggccgggga cgatcgggtt cttttatgcg gaggagcagg agggcgatga attccagcga
     3661 gaggcccgcg ccatagagga tgatgtccat gacgatgaga tcggagaagg tgaagagcac
     3721 gagaatgctg acgatgatcg aggaggcgat gatcgagata tagggagtat tgaaacgcgg
     3781 atgcagggag cagagacggg tgggcaggag ctggtcgtcg gccatgacct ggggtacccg
     3841 ggagacggat agcagcacgg cggagtagag gccgagggtg ctggccatgc cgcccgcggc
     3901 gatgagcatg ccgagccaat ggccgccgat gagttcgccc aggacgggga agccgccgtt
     3961 gaggagggcc tggtgatcga cgccgctgcg ggctacggtg agcgccgaaa aggtatagat
     4021 gccgattacg agcaggaagg cgatgcagat cgacaggatg taggagcgga cggggcgtgc
     4081 gacctcgccc gcataggtgc tggcgttgtc ccagccgatg aagttccaca tgacggtgta
     4141 gagggcgagg gagacggaat gaaaaccgta gccgccgtgt atcgtgttgc tgccggcggt
     4201 ggtggcggta gtaatagtcg tggctgcggc cgggtggagg aaggcgaggc cgaagaggag
     4261 gatgaacggt acgaagacgg ccactcccag gaggaggga
//
LOCUS       DATGFG010000353         6762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1168368, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000353 DATGFG010000000
VERSION     DATGFG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168368"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VKQ52_06730"
     CDS             complement(<1..587)
                     /locus_tag="VKQ52_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLZ86912.1"
                     /translation="MVETTFIGKTKLKEESISEKKSVNLRVYIIPRRNYLLAKRIFDI
                     TLSIFVLVFVFSWLFPILYMLIRLDSRGPVFFVQKRVGFLGRSFPCLKFRTMHVNAEA
                     NTKQATDNDPRITRMGRFLRNSNLDEIPQFLNVLAGHMSIVGPRPHMYKDCASFSSVV
                     DSYKFRNLMKPGITGLSQVKGYRGPAQTFDKIFRR"
     gene            complement(1078..1782)
                     /locus_tag="VKQ52_06735"
     CDS             complement(1078..1782)
                     /locus_tag="VKQ52_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ86913.1"
                     /translation="MSIVIPAYNEGRTIHLILDKIKQVQLTGGLRKEVIVIDDCSTDN
                     TGEAVLSYADRHPALPISYHRHEVNQGKGAALHTGIDKAGGDYIIIQDADLEYDPREY
                     NHLLEPILEGFADVVYGSRFMGAKPHRVLFFWHSIGNKMLTLFSNMFTDLNLTDMETC
                     YKLFKSPVVKGLVLREKRFGFEPEVTARIARIRDIRIYEVGISYYGRTYKEGKKINWK
                     DGFRAVYCILRYNLFR"
     gene            1906..3015
                     /locus_tag="VKQ52_06740"
     CDS             1906..3015
                     /locus_tag="VKQ52_06740"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HLZ86914.1"
                     /translation="MKIGILGTRGIPNAYGGFEQFAQYLSQGLVERGHSVWVYNSSDH
                     PYKEKEWKGIRIIHCTDWESRIGTAGQFIYDYNCLRDARRRDFDILLQLGYTSNSIWH
                     RIWPKRMVNVVNMDGLEWKRSKYGPMTRKFLKRAEGWAARRGDLLVADSIGIREHLRK
                     EYDRPSVFIPYGAEIPQEFDPAVLTRWELEPGGYFLLMARMEPENNIEMIIRGWMETK
                     KDKPLVLIGNPGNAFGQRMTATYRHEQLRFIGAIYDAPVVHSLRHYSALYLHGHSVGG
                     TNPSLLEAMACQALIAAHDNIFNRAVLEDQAFYFSSAEAVRDILEPFPDQQKGKAWKT
                     RNVEKIREQYNWPGIIDAYEKVFLSQTKNQNENRN"
     gene            2999..4117
                     /locus_tag="VKQ52_06745"
     CDS             2999..4117
                     /locus_tag="VKQ52_06745"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HLZ86915.1"
                     /translation="MRIGIDAKSIYTGPVSTRIVLQNLLPELFRLYPDVEWVVFLDKK
                     DKERGFPYQAANIHVEYVWAGMNQLSNLFVLPRYCKRLKVDLVVCQTFPSVSRHTASI
                     AFIHDVLFRDFPQFFTWKERLYFSTLPFLTRKAIRVTATTRFVAGDLVRYRYVNDPSR
                     IDIVPLGVSPTFKPLPAWDPSFLEEIKKRLALPPRFLLFVGRLNVRKNLENLLKALPL
                     LQDRDIPLVVVGKADWKEPKLEALLSDPAIGHRVLMTGGVDDKDLTGIYALCTLFCFP
                     SFAEGFGLPPLEAMASGVPVVVADRTSLPEVCGEAGNYADPEKAADIAAVIDRLLSDG
                     RLYREKKEAGIKRAAEFTWTVTAKAFRQAIDNAYKSQI"
     gene            4114..5181
                     /locus_tag="VKQ52_06750"
     CDS             4114..5181
                     /locus_tag="VKQ52_06750"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLZ86916.1"
                     /translation="MKKTILVTGGAGFVGSNLCLQLKKKYPGYSIIAFDNLKRRGSEL
                     NLTAFRENGIPFIHGDIRNAEDLQAAGSFDVLIEASAEPSVTAGLDSDPTYVINNNLY
                     GSINCFNVCLKHKAALIFLSTSRVYPIDRIERAAFVEEPTRFSFAEEQDAEGISGAGV
                     SERLNLDGARSFYGTTKLSSELFIQEYAAFYGLKAAITRFGVIAGPRQMGKTDQGVVT
                     LWMAKHFWKQSLKYIGYGGTGKQVRDLLHIDDLVELIDLQLHQIGLFEGKVFNAGGGR
                     KNSASLQEMTAICERITGHRITIDSVQATRPADLRIYITDNSNIVKTTGWQPKKSVDM
                     IFSDIFEWIRANEAQLQSILK"
     gene            5191..>6762
                     /locus_tag="VKQ52_06755"
     CDS             5191..>6762
                     /locus_tag="VKQ52_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86917.1"
                     /translation="MRLTKKYLAILLILVPVISFFGFFFRYTVNAPVNDDYPAVLQFL
                     DQYVSTHSYFEKLRLIFAQHNEHRIVFSRVWTIISFKLQKQVNFNVLSLIGNLSLIGM
                     GLVFLRKFLRLNLSLVLFFPVTAFLFNLASWENMTFAMCALSNFVVHLFILLSLSFLT
                     APTGQDKKQDRKNVLLAVLFFFAAFMTQGAGLALFPVSLLILLYKREYKNLLLYLGLA
                     ALVLLFYFYGYHKPPGTTLDIVFRDFKVRTILFVFAFLGNAFDYFLVFTNDNQESISF
                     TAVIGALFFLLFIHITRQKYYRKNPFNYSVMLFIIVTAMLAAVNRSWMGLEIAGASRY
                     RINGCIFCISLYLWLIETYSGRWLTETSSVRSKVATAAMVILAGWYFVSINLKHYEYL
                     SVREETTYLEILCTKTGAPSIVYPNNSETPTQRHLIDWSSQLHVYYLPGDKEINTYFP
                     FSRKQTDDPAGAASSGLEMTKSVHGIYRTGNDFLVDGFAFLEGRSTNHQHVFVGIKNQ
                     ADSAPVFFTSKQIPRF"
BASE COUNT         1587 a         1897 c         1675 g         1603 t
ORIGIN      
        1 tatcttctga agatcttgtc gaaagtttgc gctggacccc gatagccctt cacctgcgaa
       61 agaccggtga tcccgggttt catcaggttg cggaatttgt aggaatccac cacgctcgaa
      121 aagctggcgc agtccttata catgtgaggc ctggggccga cgatactcat atgcccggcc
      181 agtacattga gaaactgggg gatctcatcc aggttggagt tccgcaggaa ccggcccatc
      241 cgcgtaatac gggggtcatt gtcggtagcc tgtttggtat tggcttccgc attgacatgc
      301 atggtccgga atttcagaca ggggaaggaa cggccgagaa aaccgaccct tttctgcaca
      361 aagaagaccg ggcctctcga gtcgagccgg ataagcatat aaagaatggg gaacagccaa
      421 ctgaaaacaa aaacaaggac gaagatggaa agagtgatat cgaagatcct ttttgccaga
      481 aggtaattac gccgcggaat gatataaacc cttaggttaa cagacttttt ttcgctgatg
      541 ctctcttcct ttaacttggt ttttccgatg aacgtggttt ccaccataag accgaaggtt
      601 taataattta aaacgtcctt tttatttgtt aatacctatt ctaagatcca ctgaactagc
      661 caattacaac acgatttccc gtttgttcca aactccttcg acataattcc cggatgatcg
      721 tttaatgtca gcgcccgtcg atggaaactt tattttcact ccacatattt cgcattttca
      781 tatataagaa aatgctttta gcgattaact ataacacttt ttcatacaac ttattgctta
      841 ggaaaactta attttcttaa gcggcaacta tcgtatgtcg agggaaatgc ggtactcctt
      901 gaccagcctc caggggtccg gaacgacttc caggagcccc gttggaatta ttctcgtgtg
      961 tagtgtaatt taattgaaag tcaattaaat aaagtacctc gccgcaaaga aacgaaaaat
     1021 tccccgagaa aaaaatacca ctaaccggta aagtctccta acgcttagtt aaatacttca
     1081 ccggaagaga ttgtaccgaa ggatacaata gactgccctg aagccatctt tccagttgat
     1141 cttcttgcct tctttatacg tgcgcccata atacgaaatg ccgacctcgt atatgcggat
     1201 atccctgatc cgggcgatcc tcgcggtgac ctccggttcg aacccgaacc tcttttccct
     1261 tagcaccagc ccctttacca ccgggctctt gaagagcttg tagcaggttt ccatatccgt
     1321 caggttgaga tcggtgaaca tattggaaaa aagcgtcagc attttgttgc cgatggaatg
     1381 ccagaagaag agcacccggt gtggtttcgc acccatgaag cggcttccgt agacgacatc
     1441 ggcaaaccct tcgaggatcg gttccagcag atgattgtat tcccgcggat cgtattcgag
     1501 atcggcatcc tggatgatga tatagtcacc gccggcctta tcgatccccg tatgcagcgc
     1561 cgcgccctta ccctggttga cctcgtgccg gtgatagctg atcgggagtg ctggatggcg
     1621 gtccgcatag gaaagcaccg cctccccggt attgtccgtc gagcagtcat cgatcacgat
     1681 cacctccttt cgcagcccgc ccgttagctg aacctgtttt attttgtcga gaatgagatg
     1741 gatggtgcgc ccttcgttgt aggcgggaat gacgattgac aaagtcggcg gcatttgctt
     1801 gcttttgttg taagattcga atgaatttgg caaaaatagg gcaatacggt cggtacccgc
     1861 gcagtttcca atttttgaaa ctatctttac ccaattttcg aaataatgaa gatcggtatt
     1921 ctcggtacac gtggaattcc caacgcatat ggtggtttcg agcagtttgc acaatatttg
     1981 tcgcaaggcc tcgtcgaacg cggccactcg gtctgggtct acaattccag cgatcatccc
     2041 tacaaggaaa aggagtggaa gggcatccgg atcatccatt gcaccgactg ggagagcagg
     2101 atcggcacgg cgggccagtt catttacgac tataattgcc tgagagacgc gcgcaggcgg
     2161 gatttcgata tcctgctgca gctgggatat accagcaatt cgatatggca tcgtatctgg
     2221 ccgaagcgta tggtgaacgt cgtgaatatg gacgggctgg agtggaaaag aagcaagtac
     2281 ggaccgatga cgcgcaaatt cctgaagcgg gccgaaggct gggccgcccg tcgcggtgac
     2341 ctgctggtcg ccgactccat cggtatccgg gaacacctcc gcaaagaata cgaccggcca
     2401 tccgtcttta tcccctatgg cgccgagatc ccacaggagt tcgatcctgc cgtgctgacc
     2461 cgctgggaac tggagcccgg tggttatttt ctcctgatgg cgcgaatgga accggagaac
     2521 aatatcgaaa tgatcatccg gggatggatg gaaacgaaaa aagacaagcc cctcgtgctg
     2581 atcggcaatc ccggaaatgc cttcggacag cggatgaccg ccacctaccg gcatgaacag
     2641 ctgcgtttca tcggcgccat ttatgacgcg ccggtcgtac attcgttgcg tcactattcg
     2701 gccctttacc tccacggaca ctccgtgggc ggaaccaatc cctccctgct ggaagccatg
     2761 gcctgccagg cactgatcgc cgcgcatgac aatatattca accgggccgt gctggaagac
     2821 caggcctttt atttttcatc ggcggaagcc gtcagggata tcctcgaacc ctttcccgac
     2881 cagcaaaagg gcaaagcctg gaagacccgg aacgtcgaaa agatccggga gcaatacaac
     2941 tggccgggga tcatcgatgc ctatgaaaag gtctttttaa gtcagactaa aaatcaaaat
     3001 gagaatagga attgacgcga aatccatcta cacgggaccg gtaagcacca ggatcgtatt
     3061 gcaaaacctg cttcccgaac ttttccggct gtaccccgat gtcgaatggg tcgttttcct
     3121 ggacaaaaaa gataaagaac gggggttccc ttaccaggcc gcgaacatcc acgtcgaata
     3181 tgtatgggcg gggatgaacc agctttccaa cctgttcgtc ctgccgcgct attgcaaaag
     3241 actaaaggtc gacctcgtgg tctgccagac cttcccttcg gtgtcccggc atacggcctc
     3301 cattgcgttc atccatgatg tattgttccg ggacttcccg cagttcttca cctggaaaga
     3361 aagattgtat ttcagcaccc tcccctttct cacccgcaag gctatccgcg taaccgccac
     3421 tacccgcttc gtggccggcg acctcgtacg gtaccgttac gtcaatgatc cgtcccggat
     3481 cgatatcgtc ccgctcggcg tctcccccac attcaaaccc ctgccggcct gggacccttc
     3541 cttcctggaa gaaataaaaa agcgcttagc cctccccccc cggttcctgt tgttcgtcgg
     3601 cagactgaac gtaagaaaga atctcgaaaa tctcctgaaa gcccttcccc ttctgcagga
     3661 cagggatatc cccctcgtgg tggtgggaaa ggcggactgg aaggagccga agctggaagc
     3721 cctgctgtcg gacccggcca tcggccacag ggtcctgatg acggggggcg tggatgataa
     3781 agatctcacc ggcatctacg ctctttgcac cctgttctgc ttcccctcct ttgccgaagg
     3841 gttcggcctc ccacccctgg aagcgatggc ctccggggta cccgttgtgg tcgccgacag
     3901 gacctccctc ccggaagtat gcggtgaagc aggcaattat gccgaccccg aaaaggccgc
     3961 ggacatcgcg gcggtgatcg accggttatt gagcgacggc cggctgtacc gggagaaaaa
     4021 agaagccgga ataaaaaggg cggcggaatt cacctggacc gtcaccgcga aggccttccg
     4081 gcaagctatc gacaacgcat ataaatccca gatatgaaga aaaccattct tgtcaccggc
     4141 ggagcggggt tcgtcggctc caatctttgc ctgcaattaa aaaagaaata tcccggctat
     4201 tccatcatcg cattcgacaa cctgaaaaga agagggtccg aactgaacct cacggcattc
     4261 agggagaacg gcatcccgtt catccatggt gatatccgca acgccgaaga cctccaggcc
     4321 gccgggtcat tcgacgtcct gatcgaggcc tcggccgagc cctccgtcac cgcaggactg
     4381 gattccgacc ccacctatgt catcaacaat aacctttacg ggtcgatcaa ctgtttcaat
     4441 gtctgtctga agcataaagc cgcgcttatc tttctttcca cgagccgggt ctacccgatc
     4501 gacaggatcg agcgggccgc ctttgtcgaa gagcctaccc ggttctcctt cgcggaagaa
     4561 caggacgccg aagggatctc cggggccggg gtgtcggagc gcctgaacct ggacggtgcg
     4621 cgctctttct acgggacgac caaactgtcc tcggagctgt tcatccagga gtacgccgcc
     4681 ttctatggtc tgaaggccgc catcacccgg tttggcgtta tcgcgggccc gcggcagatg
     4741 gggaagaccg accagggcgt cgtcaccctg tggatggcca aacatttctg gaaacaatcg
     4801 ctgaaatata tcggctatgg cggcaccggc aaacaggtac gggatctcct gcatatcgac
     4861 gacctcgtcg agctgatcga tctgcagctc caccagatcg ggcttttcga gggaaaggtc
     4921 ttcaacgccg gcggcggacg gaagaacagc gcatcactgc aggagatgac ggccatctgc
     4981 gagcggatca ccggccaccg gatcactatc gactccgtac aggcaacccg gccggccgat
     5041 ctccgtattt atattaccga caattctaat atcgtaaaaa caacggggtg gcagcctaag
     5101 aaatcggttg atatgatttt tagtgatatt tttgaatgga taagggccaa tgaagcacaa
     5161 ttgcaaagca ttttaaaata accccggcag atgcgtctga caaaaaagta tcttgctatc
     5221 ctccttattc tggttccagt cattagtttc ttcgggttct tcttccggta tacggtcaat
     5281 gcccccgtca acgacgatta tccggccgta ctgcaattcc tcgatcagta tgtcagcacc
     5341 cactcttatt tcgaaaagct gcggcttatc ttcgcccagc ataatgaaca ccggatcgtc
     5401 ttttcgcgcg tctggacgat catttccttc aagcttcaaa agcaggtgaa tttcaatgtc
     5461 ctttccctca tcgggaatct ctcgctcatc ggcatggggc ttgtcttcct gcggaaattc
     5521 ctgcggctca acctgtcgct cgtcctcttt ttcccggtca ccgccttcct gttcaacctc
     5581 gcttcctggg agaacatgac ctttgccatg tgcgccctgt cgaatttcgt ggtccacctg
     5641 ttcatcctgc ttagtctcag ctttctcacc gcccccaccg gccaggacaa aaagcaggac
     5701 aggaagaacg tgttgctggc cgttcttttc tttttcgcgg ccttcatgac ccagggtgcg
     5761 gggcttgccc tcttccccgt ttccctcctc atcctgctct ataaaaggga gtataagaac
     5821 ctcctgctgt atctcggcct tgccgccctg gtactgctct tctattttta tgggtatcat
     5881 aagcccccgg gcactaccct cgacatcgtc ttccgcgatt tcaaggtcag gaccatcctg
     5941 ttcgtcttcg cctttctcgg gaatgccttc gactattttc tcgtctttac gaatgacaac
     6001 caggagtcga tcagctttac cgcggtgatc ggggccttgt tcttcctgtt gttcattcat
     6061 atcacccggc agaagtatta ccggaagaac cccttcaatt actccgtgat gctctttatc
     6121 atcgtgaccg ccatgctcgc ggccgtgaac cggtcgtgga tgggactcga aatagccgga
     6181 gcatcacggt acaggatcaa cggttgtatc ttttgtatct ccctctatct ctggctcatc
     6241 gaaacctatt ccggccggtg gctcaccgaa acgtcttccg tccggtcaaa agtggccacg
     6301 gccgccatgg tgatcctggc ggggtggtat tttgtttcga tcaatctcaa acattacgaa
     6361 tacctgagcg tccgggaaga aacgacctac ctcgagatcc tgtgtaccaa aacgggcgcc
     6421 ccgtccatcg tttacccgaa caactccgaa acgcctaccc aacggcatct tatcgactgg
     6481 tccagccagt tgcacgtcta ttatctgccc ggcgacaaag agataaacac ctatttcccc
     6541 ttttcccgga aacaaacgga tgatcccgcg ggcgccgcga gctccggcct ggaaatgacg
     6601 aagagtgttc acggaattta cagaaccggc aatgacttcc tggtagatgg ctttgccttc
     6661 ctggaaggaa gatccaccaa ccaccagcac gtgttcgtag gcataaagaa ccaggcggac
     6721 agcgcacccg ttttttttac atccaaacaa atcccgagat tt
//
LOCUS       DATGFG010000354         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_485337, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000354 DATGFG010000000
VERSION     DATGFG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485337"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VKQ52_06760"
     CDS             <1..507
                     /locus_tag="VKQ52_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86918.1"
                     /translation="KGRSASAPNKPDHIRLFENHKSISMFTRKHTIFGTAILPLFIAL
                     TLTAPQAFSQNYHYKIASVAQCNTLDSILAARLQVSIKQTDAGEMKFRVTILNPTEHT
                     VSIAIRRGNDLIFEDLTGKTTYDNVFNLSDLEDGNYVILISSGKEKISRNIRIQTETK
                     VDRQLTVN"
     gene            611..1741
                     /locus_tag="VKQ52_06765"
     CDS             611..1741
                     /locus_tag="VKQ52_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HLZ86919.1"
                     /translation="MRRHTLRPRNNWQKEVEKLGFGFHTTNVPYWDESACYGFPMDEI
                     LLIERASAELWDCCLGAVQYVIDNNLYHRFAIPEAFIPYIERTWMEDHPAIYGRFDFC
                     FKDGKLKLLEFNADTPTSLYEAGIIQWFWLQDYDKSKDQFNSIHEKLIGYWKYLKAYL
                     YPGDLHFACVKASLEDLTNTEYLRDCAIQGGLRTKLIYVDDIGWDSAGEVFVDRQDQP
                     VKNIFKLYPWEWMIRDDFGANLLADRNKTFWIEPAWKMILSNKAILPVLWELYPSCPW
                     LLPAYTEPGHLSDYVKKPILSREGANIELVSADTILQATTGEYGAEGYIYQELATLPD
                     FDGNHPVIGSWIIGQEPAGIGIRESNTLVTDNLSRFIPHFIS"
     gene            complement(1734..>2760)
                     /locus_tag="VKQ52_06770"
     CDS             complement(1734..>2760)
                     /locus_tag="VKQ52_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLZ86920.1"
                     /translation="ANLRDILAPFSFALFLAILLNPLVDRLEWLRVPRVPAIGLALLV
                     AIVVIAGVWYFLATQMMHFTSQLPALERKTEELVSRLQQQLARRIPLERQNQYIAEAK
                     AGIKPLIGQTLGSVVGTLATVFLLPVYTFLLLYYKTLILNFLYDIFAKENEEEVRTVL
                     RQTRGAIQSYMYGLLIEGLIVASLNTMALFILGVPYAILLGVMGAILNVLPFFGGILA
                     ALLPMVVATIVRDGYSTQIWIGVSYMSIQFIDNHFLIPYIVSAKVRINALISIVVVLL
                     GGAVWGLSGMFLSIPFIGVLKIIFDRIPEMKPWGRLLGDETPSRLVGIRGQVRRRRGG
                     AGAGGVS"
BASE COUNT          727 a          936 c          591 g          506 t
ORIGIN      
        1 aaaggcagaa gcgcttccgc ccctaacaaa ccggatcaca tccggttatt cgaaaaccat
       61 aaatccatta gtatgttcac ccggaaacac accatcttcg gaacggctat tcttcccctt
      121 tttatcgccc tgaccctcac cgctcctcaa gccttcagcc aaaactatca ctataagatc
      181 gccagcgtcg cacaatgcaa tactctggac tcgatcctgg ctgcccgttt gcaggtgagt
      241 attaaacaga ccgacgccgg tgaaatgaag tttcgcgtca ccatcctgaa ccccacggaa
      301 cacacggtat ccatcgcgat ccgtcgcggg aacgacctca tcttcgaaga cctgacagga
      361 aaaaccacct atgacaatgt cttcaacctc tccgacctcg aagatgggaa ttatgtaata
      421 ctgatcagca gcgggaaaga aaagatatcc cggaatatcc gtatccagac cgagaccaaa
      481 gtagaccggc agctcacagt gaattaactg tgagcgcctg gcggcaatag caacacgata
      541 acaaaaaagg gctggcaagt ctcgccgccg gcggttatct ttattccggc cctccggccc
      601 aacgctagcc atgagacgac acaccctccg gccccgcaac aactggcaaa aggaagtaga
      661 gaaactcggt ttcggcttcc acaccaccaa tgtcccctac tgggacgaaa gcgcctgtta
      721 cggattcccg atggacgaga tcctgctcat cgaaagagcg tcggccgaac tctgggattg
      781 ctgcctcggc gccgtgcaat atgtcatcga taacaatctc tatcaccgct tcgccattcc
      841 ggaggctttc attccttata tcgaaagaac ctggatggaa gatcatccgg ctatctatgg
      901 ccgctttgac ttctgcttca aggacgggaa gctaaaattg ctcgaattca acgccgatac
      961 ccccaccagc ctttacgaag ccggcatcat ccagtggttc tggctgcagg attacgacaa
     1021 aagcaaagac cagttcaata gcatccacga aaaactcatc ggttattgga aatacctgaa
     1081 ggcctatctc taccccggcg acctccactt cgcctgcgta aaggcctcac tggaagacct
     1141 caccaatacg gaatacctga gggactgcgc catccagggc ggtctccgga caaaactcat
     1201 ctatgttgac gatatcggct gggactccgc cggcgaggtc ttcgtcgaca gacaggatca
     1261 accggtaaag aacatcttca aactctatcc ctgggaatgg atgataaggg atgacttcgg
     1321 cgccaacctc ctcgccgatc gcaataagac cttctggatc gaacccgcct ggaaaatgat
     1381 tttatccaac aaggccatcc tcccggtcct ctgggaactc tatcccagct gcccctggct
     1441 cctgccggcc tacaccgaac ccggccacct cagcgactac gtaaaaaagc ccattctctc
     1501 ccgcgaaggc gcgaatatcg aactcgtcag cgcggacact atcttgcaag ccacgacagg
     1561 cgaatacggc gccgaagggt atatctacca ggaactcgcc accctccccg acttcgacgg
     1621 caaccacccc gttatcggca gctggatcat cggccaggag ccagccggca tcggcatccg
     1681 cgaaagcaac accctcgtca ccgacaacct cagccgcttc atcccccatt tcatcagcta
     1741 actccccccg cacctgctcc accccgcctc cgcctcacct gcccccgtat ccccaccaac
     1801 cgcgacggcg tctcatcccc caacaaccgc ccccagggct tcatctccgg gatccggtcg
     1861 aaaatgatct tcaacacccc tataaacggg atcgacaaaa acatccccga caacccccac
     1921 accgccccgc caagcaagac cacaacaatc gatatcagtg cattgatcct caccttcgcc
     1981 gacacgatat acggaataag aaaatgatta tcgataaact ggatcgacat ataactcaca
     2041 ccgatccaga tctgcgtact atacccatct ctcacgatcg tcgccaccac catcggcagc
     2101 aacgccgcga gtattccccc gaagaacggc agcacattca ggatcgcccc catcacaccc
     2161 agcaagatgg catacggcac ccccaaaatg aataacgcca tcgtattcag actggccacg
     2221 atcagcccct cgatcagcag cccatacata taactctgga tcgcaccccg agtctgccgc
     2281 agtaccgtcc gcacctcctc ctcattctcc ttggcgaaga tgtcgtaaag aaaattcaat
     2341 atcagcgtct tgtaatacaa cagcagaaaa gtgtacaccg gcagcaaaaa caccgtcgcc
     2401 agcgtcccga ccacggaacc cagcgtttgc cctatcagcg gcttgatccc cgccttcgcc
     2461 tcggcgatat actggttctg gcgctccagc gggatcctcc tcgccagctg ctgctgcagc
     2521 ctcgacacca gctcctccgt cttccgctcc agcgccggca gctgcgaagt aaaatgcatc
     2581 atctgcgtcg ccaggaaata ccaaaccccc gcgatcacaa caatggccac caacaacgcc
     2641 agcccgatcg ccggcacccg cggcacccgc aaccactcca gccggtccac cagcgggtta
     2701 agcaggatag ccaggaacag cgcaaaggag aaaggagcaa ggatatcgcg cagattagcg
//
LOCUS       DATGFG010000355         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1065884, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000355 DATGFG010000000
VERSION     DATGFG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065884"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1895
                     /locus_tag="VKQ52_06775"
     CDS             135..1895
                     /locus_tag="VKQ52_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86921.1"
                     /translation="MTQFIKTLVRRRKKIPLTALSLFSLSCLLLLAALPVEVRAQSSY
                     LPQGSKFDHFLDRMEILQQTDPDLNISTAKPISRRLAVRVAQLSDSLHKFYPYDELYH
                     LSAVDRANLSSLLMNNIEWVTTNKDSFMSKRPWLQTFYKNKANFYEVEDKDFFLAVDP
                     AIQQTQSYESGNSQRVFLNSKGLTLRGLIGGKLGFSAYLTDNQERGPSYFQQRVAQYD
                     AVPGAGYFKSFGKTGFDYFDNRASIYFNTWKYVDWQFGYDKNFIGNGYRSLFLSDYSA
                     PYLFLKFNTRIWKMNYQTIYMELINQHNTHTDYQYPKKYAVVHHLNVNAAPWLTVGLY
                     ENVVFSRADHYDFSYLNPVIFLVSAQQQNGSPDKTTAGLDWKLNIGHAVQFYGQLLIN
                     EFILHQILHYGQGYWANKQGLQLGLKYVNAFEVKNLDLQVETNFVRPYTYQHDDTVAN
                     YSNYNQPLAHPLGANFYEFITIAHYQPAYKWNLEGKLIYYRQGLDSAGVNFGSDIFEN
                     YTTRPRDYGFQIGSGVPATCVLVSGLVSYQWKENLFLELSAQYRNYSVHDPSHTYHSS
                     SAAMVTAGVRINMFRREYDY"
     gene            complement(1939..2565)
                     /locus_tag="VKQ52_06780"
     CDS             complement(1939..2565)
                     /locus_tag="VKQ52_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HLZ86922.1"
                     /translation="MESYTRQQEIVNGLIHGAGVLFGVAGIPVLISIANVHHNTPAII
                     GAGIYGFCFLLVFSVSTIYHFSSDPKVKMIFKIFDHIGIYFFIAGTYTPFLLVYMNNP
                     FGHTLLAVLWSLTVAGTLFKVWYTGRFEIISTIIYLAMGWIMFAGGNKFFEQLPYPVL
                     VFVIIGGALYSIGVFFYLWDRYTYTHAVWHVFVLAAAMCHYVAVLMTM"
BASE COUNT          676 a          762 c          668 g          646 t
ORIGIN      
        1 cccatttttt cgatccgccc gtttttcagt agtaaatctg ccgtactaat cctaccttcg
       61 tttactatgg cgatattctt gatcaggtga ttacgcataa cgaatattag atcctccctt
      121 tatttgatag cattatgacg caatttatta aaacccttgt aagacgccgg aaaaaaatac
      181 cgctgactgc tctctccctg ttttcccttt cctgcctcct tttgctggcc gcgctgccgg
      241 tagaagtccg ggcacagtcc agctacctgc cgcagggttc taaattcgac cactttctgg
      301 acaggatgga gatccttcaa caaacggatc ccgacctcaa tatcagcacg gccaagccga
      361 tcagccgccg gcttgccgtc cgggtggccc agctatccga ttcgctgcat aaattctatc
      421 cctacgacga actctaccat ctgtcggcgg tggaccgggc caatctctcc tctctcctca
      481 tgaacaatat cgagtgggtg acgacaaaca aggacagctt catgagcaaa cgcccctggc
      541 tgcagacttt ttataagaac aaggctaatt tctacgaggt cgaggacaag gatttcttcc
      601 tggcagtgga tcccgccatc cagcagaccc aatcctatga gagcggcaac agtcaacggg
      661 tgttcctcaa ttccaaaggc cttaccctcc ggggcctgat cggcgggaaa ctcggcttct
      721 cggcctatct taccgataac caggaacgcg gcccctccta tttccagcag cgcgtcgccc
      781 agtacgatgc cgtacctggt gcaggctatt tcaaatcctt tgggaagacc ggtttcgact
      841 atttcgataa ccgggcctct atctacttca acacctggaa atatgtcgac tggcaattcg
      901 gctatgacaa gaactttatc ggtaacggtt atcgcagcct cttcctcagt gattacagcg
      961 ccccctatct cttcctgaag ttcaataccc gcatctggaa gatgaattac cagaccattt
     1021 atatggagct gatcaaccag cacaacaccc ataccgacta tcagtatcca aaaaagtatg
     1081 ccgtcgtcca ccatctgaat gtcaatgcag ccccctggct gaccgtcggg ctctacgaga
     1141 atgtggtctt cagccgggcc gatcactatg atttctccta tctgaatccc gtcatcttcc
     1201 ttgtatccgc ccagcaacag aacggcagcc ccgacaagac gacggccggc ctcgactgga
     1261 agttgaatat cggtcacgcg gtgcagttct atggccagct cctgatcaac gagttcattc
     1321 ttcaccagat actgcactac ggacagggtt actgggcgaa caaacaggga ctgcagctcg
     1381 gtctgaagta tgtaaatgct ttcgaggtga agaacctcga tctccaggtc gaaaccaatt
     1441 ttgtccgacc ctatacttac cagcatgacg acaccgttgc caattacagc aactacaacc
     1501 agccgctggc gcatcctctc ggggcgaatt tctatgagtt cattaccatc gctcactacc
     1561 agcctgccta taaatggaac ctcgagggca agctcatcta ttaccggcag gggctggact
     1621 ccgccggggt caatttcggc agtgatatct tcgagaatta tactacccgg ccccgcgact
     1681 atggattcca gatcggcagc ggggtgcccg ccacctgcgt gctggtatcg ggactggtct
     1741 cgtaccagtg gaaggaaaat ctcttcctcg agctttcggc ccagtaccgc aactattccg
     1801 tccacgatcc cagtcacacc tatcacagct cgagcgcggc gatggttacg gccggggtcc
     1861 ggatcaatat gttccggcgg gaatatgatt attaaggccg cttagcctat tgagaccgat
     1921 ttacctattg agatcgcatt acatcgtcat cagcacggct acatagtgac acatggctgc
     1981 ggccaggaca aaaacatgcc ataccgcatg ggtataggta taccggtccc acaggtaaaa
     2041 gaataccccg atggaataca aggcgccccc gatgatgacg aaaaccagca cggggtatgg
     2101 caattgttca aagaatttgt tgccgcccgc gaacatgatc catcccatgg ccaggtaaat
     2161 gatcgtggaa atgatctcga accgcccggt ataccaaact ttgaacaggg tgcctgccac
     2221 ggtgaggctc cataggacgg ccagcagggt gtggccgaag gggttgttca tatagacaag
     2281 gagaaaaggg gtgtaggtgc ctgcaataaa gaaatagata ccgatgtgat cgaagatctt
     2341 gaagatcatt ttcactttcg ggtcactgct gaaatgatag atggtgctga cgctaaagac
     2401 gaggaggaag caaaagccat agatgccggc cccgatgatc gccggagtat tgtgatggac
     2461 gttggcgatg ctgatcagga caggtattcc cgcgacgccg aagaggacac cggcgccgtg
     2521 tatgagtccg ttgacaattt cctgttggcg ggtatagctc tccatggtac agggattggc
     2581 actttaaagt agaacagcgc attacccggg acggttcact atggggaaaa tatctagccc
     2641 tcgacgatga tcgagaaaac gatcatcctg gacttcagct tgtcgatcac gttcgagtat
     2701 tgcaggctcg gatcgcggct gtggatgttc ttgataccct cgcttatctt ca
//
LOCUS       DATGFG010000356         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_573524, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000356 DATGFG010000000
VERSION     DATGFG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573524"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VKQ52_06785"
     CDS             <1..885
                     /locus_tag="VKQ52_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLZ86923.1"
                     /translation="FRQHDEEQILDGLAASGPDRFQSWLGSLQRPSPAFDTLRAAIGR
                     YLDSGDSRRVAQLAHTLNTFRRIRHFQFDRFIVVNIPSAGLRYYSADTLTLQMRVVAG
                     QTSKRTPRFAAWCTGLVLYPYWNIPRRIAIKEMLPLFRRVPGLATVMDIQVLDEKGRT
                     IDPAGIDWKTVSPADFPYTLRQAPGCENALGVLKFELNDPFDVYMHDTNLKRAFGANY
                     RYLSHGCIRLEKASQLGNLLLDHRLDTAFLAACFKDQRPSPVPLAKPVPVFVVYLTAL
                     PDSTGRIIWLKDIYHLRH"
     gene            947..1309
                     /locus_tag="VKQ52_06790"
     CDS             947..1309
                     /locus_tag="VKQ52_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86924.1"
                     /translation="MEDFAKVENLVDHVKDYMHVRVDEARLGIAERSSGAVAFLAAGV
                     AVTGIFTLCFTFIGIGAALLIGRVLDDLVSGFLIVAAFQFLLGLIIWRARQRLIRIPI
                     MNAILQQLLSNKTNDEDN"
     gene            1296..1529
                     /locus_tag="VKQ52_06795"
     CDS             1296..1529
                     /locus_tag="VKQ52_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86925.1"
                     /translation="MKTIKSITHLREEQLRLARRRASLESTIRGDWDTLRHHFEPAAY
                     AREAFFNGLSWLGKRIFSTEGRTGRNRTKTHSS"
     gene            1541..2362
                     /locus_tag="VKQ52_06800"
     CDS             1541..2362
                     /locus_tag="VKQ52_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86926.1"
                     /translation="MKKIIVPFEGGAFRTELLEFVKDLNCRSKVFLTAAFVPEVDYAQ
                     LWTPSATVESDMYLPAAVDEDKLIARNSTKLSRFCETHSIGLRIHEDRFDFALAAIKK
                     ETRFADLLLLSSRHFFEVIDESQPNAYMTRILHESECPVMLLPEEPRMPGEIVLAYDG
                     TSASVYAIRQFAYLFPEFANIRTTLIYVDPKGKGEIPDHDSVRELGRLHFRNFRMLRL
                     SMCTDTFYDTWIGLTTNPWLVAGAFGRSDWSRLLCHSFISRMIREHNVPIFLAHP"
     gene            complement(2378..3061)
                     /locus_tag="VKQ52_06805"
     CDS             complement(2378..3061)
                     /locus_tag="VKQ52_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ86927.1"
                     /translation="MANEVLKADRLTKFFYEPAKVQVLYDISLTVEKGQFVTIMGKSG
                     CGKSTLLYLLSTLDTDYGGEVSIMGTRVTGLGEDRLAKFRNEHIGFVFQFHFLLPEFT
                     ALENVMLPALKSGKWDRKTIEDRAMDKLREMEMEGYAQQPSGKLSGGQQQRVAIARAL
                     INDPDLILADEPTGNLDSANSDRVIAIFRQLAAQGNTIIVVTHDRDLAEKSDRIVEMS
                     DGRIAPKGS"
     gene            complement(3072..4334)
                     /locus_tag="VKQ52_06810"
     CDS             complement(3072..4334)
                     /locus_tag="VKQ52_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86928.1"
                     /translation="MRLPVNFRIAGVQLLSRRRQTLIAMLGVMFGIAMFIFMISFMNG
                     VNKIIQDIMLSVTPDIHLYNDLRTDYRSSVTSRFYEEDSSRWIIVRHPRPRQTNINLK
                     DAPGIISGLRGYPGVLAVSPLVSVQILFNYGPVQLGVVADGVDMREEDRLFDLSGKMI
                     SGRPRDLLTIENGILLGKKLAEKLHVMMGDIVTATASSGAQMQFRVVGIFEFGLSQVD
                     EGKVYISLPNMQQLLGRNRDYITDIRIKLKDLGQAKALAAVFTKKYGYRADDWETVNA
                     SVKAGNLIRDTLTYVVSFTMLLVAGFGIYNIMNMVILGKMKDIAILKAQGFDRRDITQ
                     IFLSQSLSIGVAGGVAGLLLGFGLSYGLSRMPFPTDEYMLIKFYPVTFEAKHYLLGAG
                     FGLLTTFLAGWLPARKASRVDPVAILRT"
     gene            complement(4331..>4640)
                     /locus_tag="VKQ52_06815"
     CDS             complement(4331..>4640)
                     /locus_tag="VKQ52_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86929.1"
                     /translation="MIYPVMNELDQTFRVDARFTGVSMPAFIHSSAEANIIIQRKANS
                     LVLPRSALAGNDSVWVRESGGERKVRVRLGISTPDDVEILAGINEQTAVRAVPKSAAP
                     "
BASE COUNT          980 a         1630 c         1087 g          943 t
ORIGIN      
        1 ttccgtcaac acgacgaaga acagatcctc gatgggctcg ccgcttcggg ccccgatcgc
       61 ttccaaagct ggctcggcag cctgcaaaga cccagtcccg ccttcgatac cctccgggcg
      121 gccatcggcc gttatctcga cagcggcgat agccgccgcg tcgcccaatt ggcccacacc
      181 ctcaacacct ttcgccgtat ccggcatttt cagttcgatc gctttatcgt cgtcaacatc
      241 ccctccgccg gcctgcggta ttactccgcc gataccctca ccctccagat gcgcgtcgta
      301 gccggccaga cctccaaacg caccccccgc ttcgccgcct ggtgcaccgg cctcgtcctc
      361 tacccctact ggaatatccc ccggcgcatc gccataaaag agatgctgcc gctcttccgt
      421 cgcgtgccgg gactcgcgac cgttatggat atccaggtcc tcgacgaaaa aggccggacc
      481 atcgatccgg ccggcatcga ctggaaaaca gtgtcgccgg ctgactttcc ctataccctc
      541 cgccaggcgc ccggctgcga gaacgccctc ggcgtcctca aattcgaact caacgatccc
      601 ttcgacgtct acatgcacga taccaatctc aaacgggctt tcggcgcaaa ctaccgctat
      661 ctcagccatg gctgcatccg gctcgaaaaa gcgtcccagc tcggcaacct gctcctcgat
      721 caccggctcg acacggcttt cctggccgcc tgcttcaaag accagcggcc atcccccgtc
      781 cccctggcaa aacccgtccc tgtcttcgtc gtctacctca ccgccctccc ggactccacc
      841 ggccggatca tctggctgaa ggacatttat catctccggc actgacctct gtcatatacc
      901 cggcatccct tccgccgtag atttatacct gtcaaaaaac aacaatatgg aagactttgc
      961 aaaagtggag aacctcgtcg atcacgtgaa ggactatatg catgtccggg ttgacgaagc
     1021 acggctcggc attgccgaac gcagctccgg ggccgtcgcc tttcttgccg ccggcgtcgc
     1081 cgtcaccggg atctttaccc tctgttttac cttcatcggc atcggggcgg ccctgctgat
     1141 cggccgcgtt ctcgatgacc tcgtctccgg cttcctgatc gtcgccgcct tccagttcct
     1201 gctggggctg atcatctggc gggcaagaca aagactgatc cgcattccca taatgaacgc
     1261 catcctgcag caattactct caaacaagac aaacgatgaa gacaattaaa agcatcaccc
     1321 acctccgcga ggaacaactg cggctggcac gccgccgcgc cagcctcgaa tcgaccatcc
     1381 gcggagactg ggataccctg cgccatcact tcgaaccggc cgcctacgcc cgcgaggcct
     1441 ttttcaatgg cctgagctgg ctcggcaaac gcatcttttc aacggaaggc cgcaccggcc
     1501 gcaaccgcac aaaaactcat tcctcttaac accggcgata atgaaaaaga taatcgttcc
     1561 attcgaaggc ggcgccttcc gtaccgaact cctggaattt gtaaaagacc tcaactgccg
     1621 gtcgaaggtc tttctcaccg cggcctttgt ccccgaagtg gattatgccc aactgtggac
     1681 cccctcggcc accgtggaaa gcgacatgta tctccccgcg gccgtcgacg aggacaaact
     1741 catcgcccgc aacagcacca aacttagtcg tttctgcgaa acccattcca tcgggctgcg
     1801 tatccacgag gaccggttcg atttcgccct ggccgcgatc aaaaaagaga cccgcttcgc
     1861 cgaccttctc ctgttgagca gccggcattt tttcgaagtg atcgatgaga gccaacccaa
     1921 cgcctatatg acaaggatcc ttcacgaatc ggaatgcccg gtgatgctgc ttcccgaaga
     1981 gccccgcatg cccggggaga tcgtcctggc ctatgacggc acttccgctt ccgtatatgc
     2041 catccgccag ttcgcctacc tcttccccga atttgccaac atccggacga cactcatcta
     2101 cgtcgacccg aaagggaaag gcgagatccc cgaccacgac tcggtccgcg aactcggcag
     2161 gctccacttc cggaacttcc gcatgctccg cctcagcatg tgcaccgaca ccttctatga
     2221 tacctggatc ggcctgacga cgaacccctg gctcgtcgcc ggcgccttcg gccgctccga
     2281 ctggtcccgc cttctttgcc acagctttat ctcccgcatg ataagagaac acaacgttcc
     2341 catcttcctg gcccatccct gatcctcatc agcctggtca ggaacccttc ggggcgatcc
     2401 tcccatccga catctcgacg atccggtccg acttctccgc gaggtcccgg tcatgtgtga
     2461 ccacaatgat cgtattcccc tgcgccgcca actggcggaa gatcgcgatc acccggtccg
     2521 aattcgccga atcgaggttt cccgtaggct cgtcggccag gatcaggtcc ggatcgttga
     2581 tcaatgcccg cgcgatcgcc acccgctgct gctgcccgcc ggataacttc cccgacggct
     2641 gctgcgcata tccttccatc tccatctccc gcagcttgtc catcgcccgg tcctcgatgg
     2701 tctttctatc ccatttacct gatttcaagg ccggcagcat cacattctcc agcgccgtga
     2761 attccggcag gagaaaatgg aattggaaca cgaacccgat atgctcgttg cgaaacttcg
     2821 ccagccggtc ctctcccaaa cccgtgaccc tcgtacccat gatcgagacc tcccccccat
     2881 agtccgtgtc cagcgtcgac aacaggtaca gcagggtcga tttgccacac ccggacttgc
     2941 ccatgatggt cacgaactgc cccttctcca ccgtaaggga aatatcatac aacacctgca
     3001 cttttgccgg ttcatagaag aatttcgtca gcctgtccgc cttgagcacc tcatttgcca
     3061 tagcctgttg attaagtccg taagatggcc accgggtcca cccgcgaagc cttcctcgcc
     3121 ggcagccagc ccgccaggaa ggtcgtcaga agtccaaacc ccgcacccag caaataatgt
     3181 tttgcctcga acgtcacggg gtagaatttg atcagcatat attcgtccgt cgggaagggc
     3241 atcctcgaca acccatagga gaggccaaaa cccagcaaca accccgccac cccgcccgcc
     3301 accccgatgc tgagcgactg cgacaggaag atctgcgtga tatcccgccg gtcgaacccc
     3361 tgggccttga ggatggcgat atccttcatc ttcccgagga tcaccatatt catgatattg
     3421 taaatgccaa accccgcaac aagcaacatc gtaaagctga cgacataggt cagcgtatcg
     3481 cggataagat tgccggcctt caccgaggca ttcaccgtct cccagtcatc cgcccggtaa
     3541 ccatactttt tcgtgaaaac ggcggccagc gccttcgcct ggcccagatc cttcaatttg
     3601 atccggatat ccgtgatata gtcccggttc ctgcccagca actgctgcat attcggcagg
     3661 ctgatataca ccttcccttc atccacctgg gacaacccga actcgaagat gcccactacg
     3721 cggaattgca tctgcgcccc cgatgaggcc gttgccgtca cgatatcccc catcatgaca
     3781 tgcagtttct ccgccagttt cttccccagc aggatgccgt tctcgatcgt cagcagatcc
     3841 cgcggccgcc cgctgatcat ctttcccgac agatcgaaaa gccggtcctc ctcccgcata
     3901 tccaccccat cggcaaccac acccaactgc accggcccat agttgaaaag gatctgcacg
     3961 gacacgaggg gcgataccgc gaggaccccc ggatatccac gcagccccga tatgataccc
     4021 ggcgcatcct tcaggttgat attcgtctgc cggggccgcg gatgacggac gatgatccac
     4081 cgcgaagagt cctcctcata aaaccggctc gtcactgaag aacgataatc cgtcctcagg
     4141 tcattgtata gatggatgtc cggcgtcacc gacaacatga tatcctggat gatcttgttg
     4201 acgccgttca taaaactgat cataaagatg aacatggcga tcccgaacat gacccccagc
     4261 atggcgatca gcgtttgccg cctgcgcgat aacagttgca cgcccgcgat ccggaaattg
     4321 accggcagtc tcatggcgcg gcggatttag gaaccgccct cacggccgtc tgttcgttta
     4381 tccccgccag gatctccaca tcgtccgggg ttgagatccc taaccgcacc cgcaccttcc
     4441 gttccccacc gctctcccgg acccatacac tgtcgtttcc cgccaacgcc gaccgcggca
     4501 ggacaagact attggccttc cgttgtataa tgatattcgc ttccgccgaa ctgtggataa
     4561 aggccggcat ggagaccccc gtgaaccgcg catcgacccg gaacgtctgg tccagctcat
     4621 tcatgacagg atagatcatg
//
LOCUS       DATGFG010000357         6958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_451719, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000357 DATGFG010000000
VERSION     DATGFG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451719"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VKQ52_06820"
     CDS             complement(<1..1057)
                     /locus_tag="VKQ52_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HLZ86930.1"
                     /translation="MDYIRQYKSFINSHYLNGAIRITVGITLPAILLSQFNDLAAGIS
                     VSIGAMCVGNTDNPGPIHHRRNGMIACVLIIFLVTLLVGLASGSHVWTGILVFVFCFI
                     FSFMGVYGTRASSIGVNALLVMVLNIDRQRTGLDSLITALYVLSGGVWYTALSLLLYS
                     FRPYKLVQQAIGDCVQATAGYLRIKAAFYSQDTDYDEDYRQLVESQVSIHAKQDLVRE
                     LLFKSRNIVKESTHVGRVLMMIFLDIIDLFERIMTSQQDYRMLHRFFDDSGLMQECRR
                     LILDLSAELDEIGIAIMSGKPSVENRELPTRIRAVRDQLNQYRDGHRNAENAEAFTGL
                     GNILDSIEDLGLRMHTLH"
     gene            1167..3623
                     /locus_tag="VKQ52_06825"
     CDS             1167..3623
                     /locus_tag="VKQ52_06825"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HLZ86931.1"
                     /translation="MRILSLFLLLILSYGSLQAQQVSGLTQDDRGKPLSGASVALKNA
                     KDSSVVKLGISGSSGQYEFSPVAPGHYFVTVTHIGFVPQNSASFLTKQEGRIQTPAIS
                     LSRMPKELREAVVTGRKPLVEVKPDKIVLNVEGSINEVGSDALELLRKSPGVTVDKDN
                     NLSVNGRNGVQVYIDGRPTYLSVANLGDYLKTLQSSSIASIEIISNPSAKYEAAGNAG
                     IINIRLKKNQAYGTNMTVSAGYNIGTYSKYNGAFSFNHRDQRFNIFGDYSYNNARNEG
                     YSTLFRTQLDTFFLQSTTLVTTNSTHNYKAGIDYFIDKKSTLGFIVNGVSSDYHVRSN
                     SATPIVYLPTNGTSRILQANNRTDGSRDNLNLDLNYRYADSSGHELNIDADYGLYHLR
                     SNQFQPNDYFDSTRKSLLYSNDYNMLSPSDIYIYSVKADYELRWAKGILDIGLKSSYV
                     TTRNNFQEYNITNPTGGGTESVLDTLSSNDFNYKENINALYANYKRTLKGWIIQGGLR
                     AENTNGKGISTGYKESAGPSDPFVPYDSSFTRHYTDLFPSASVTWNSHPTKQWTLSYS
                     RRIDRPSYQSLNPFEFKLDDYTFSRGNTLLRPQYTNSVGLTFMYHYKLTANLNYSHTK
                     DLATTIIDTTQGSKMISRQENLATQDVLSLTISYPFQYKWYSAFVNITGFYQLNKANF
                     GAGRTIDLNVFNTTVYTQHTLRLGRGWSGEITQYYTSPNVWLATLRASSLWNIDAGLQ
                     KTIFGGNGSFKVAVTDIFHTLQWTATSDFAGQDIRASGGSETRMLKLYLTYRFGNKHV
                     KAARRHSNGDEEESKRVKTQ"
     gene            3732..5177
                     /locus_tag="VKQ52_06830"
     CDS             3732..5177
                     /locus_tag="VKQ52_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HLZ86932.1"
                     /translation="MTAITDDIRTLFSGFSPTPLTELEKIPQSGSNRMYFRILTGNGS
                     YIATYNDNIRENRTFVQYSRHFRAKGCPVPEIYSINDAYTIYLQEDFGDQSLLKELEK
                     EGHNERVYTFFQKSLEQLARLQILGDEGLDYSWTITSREFGKQAILSDLLYFKYYFLD
                     TLQIPYDKEKLIDDFEALSTYLTHVDHKYFMFRDFQSRNIMLKDEAVHFIDFQGGMKG
                     ALQYDVASLLWQAKAELPDTWKDNLLDYYMDCVESILDTKIDRTRFISQYNGYVLIRL
                     LQVLGAYGFRGLFERKAHFLISIPLALRNFKWFLGNKQVGIVLPEFERLLGLMVQEEV
                     ISRFEPLQADENTPLVVHINSFSYKKAHPVDDSGNGGGFVFDCRGILNPGRIPEFKTQ
                     TGRDKPVKDFLEQQTKMPEFLNSVYDLVDISVEDYIKRGFGSLQVSFGCTGGQHRSVY
                     AADALARHLRNKFHVRIHLQHIEQEAKNWIN"
     gene            5185..6093
                     /locus_tag="VKQ52_06835"
     CDS             5185..6093
                     /locus_tag="VKQ52_06835"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLZ86933.1"
                     /translation="MYYLVYGSLYLLSLLPMWLLYGLSDGIAFVLYAVIRYRRGVVLS
                     NLRIAFPEKTDVQRLAIAKRFYRNFTDNFIETIKLLSAGEAWLQERFVLDNPELLDHF
                     YDQGRKLQLHLGHLFNWEIANVAIPTRTRYTFIVAYMPVENKAFERVFLRLRGRTGTV
                     LLPATKMQRAILPYRNTQYMLALVADQAPGGPDNSYWLNFFGQPTPFVRGPERGARIA
                     DIPAVFVRFYKPRRGHYRAELITIADHPAALPEGELTRRYRRLLEEGIRQEPAGWLWS
                     HKRWKFTWTPGFRPFWIDEESFPPNA"
     gene            6283..6945
                     /locus_tag="VKQ52_06840"
     CDS             6283..6945
                     /locus_tag="VKQ52_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ86934.1"
                     /translation="MTTFNPGVVLVDDHVLLRNGLASLIRGFGRFDVLFEASDGKDLI
                     RQLRISRRPDIILLDIGMPEMDGYETACYLRRHYPEIRVLALTMSETDSAIVRMLKNG
                     ARGYILKDIDSDGLRRALDAMVEKGYYYSELVTDRLIDTINYVGEPEKRIREILLLND
                     RELEFMKLVCTECTYKEIADRMYLSPRTIDGYRDALFEKLNVRTRVGLVLYAIRNGIV
                     SA"
BASE COUNT         1705 a         2166 c         1674 g         1413 t
ORIGIN      
        1 catggagggt atgcatccgc aggcccaggt cttcgatgct gtcgaggata ttgcccagcc
       61 cggtaaacgc ctccgcattt tcggcgttgc gatggccgtc gcggtattga ttcaactggt
      121 cccgcaccgc gcggatccgc gtggggagct cccggttctc gacggaaggc ttcccgctca
      181 tgatggcaat gccgatctca tccagttcgg ccgaaagatc gaggatcagc cggcggcact
      241 cctgcatcag cccggaatcg tcaaagaaac gatgcagcat gcgataatcc tgttgcgagg
      301 tcatgatcct ttcgaaaaga tcgataatat ccaggaagat catcatcagc acacgcccga
      361 catgcgtcga ctcctttacg atgttccggc ttttgaacaa taactcccgc accaggtcct
      421 gcttcgcatg gatggagacc tggctctcca ccagctgccg gtaatcctca tcgtagtcgg
      481 tatcctgcga atagaaagca gcctttatcc gcagatagcc ggctgtagcc tgaacgcagt
      541 cgccaatcgc ctgctgaacg agcttgtacg gccggaagct gtataacagc aggctcaaag
      601 cggtgtacca gacgccgccc gacagtacat acagcgccgt gatcaggctg tcgagccccg
      661 tacgctgccg gtcgatattc aacaccatga ccagcagcgc attcacaccg atagagctgg
      721 ccctggtgcc atagacaccc atgaacgaaa agataaaaca gaacacgaac acgaggatgc
      781 ccgtccacac atgcgaaccc gacgccaacc cgacgagcag ggtcaccagg aagatgatta
      841 acacacacgc tatcatacca ttacggcgat gatggatcgg cccggggtta tcggtattcc
      901 cgacacacat cgcaccgatg gatacggaga tgccggccgc aagatcattg aattgactga
      961 gcaggatcgc aggcagcgtg atccctactg taatacgtat agcgccattc aggtaatggc
     1021 tgttgataaa actcttatat tgcctgatat aatccatgtt gatcgcaaaa tttttgatcg
     1081 ccgaagtgct ccttaaaata aatttttctt tccttcgtat aaatgctttt gtgcttacct
     1141 cctgacaaac ctataatgcg tcaaacatga gaattctctc tttattccta ttgctcatcc
     1201 tctcctatgg aagcctgcaa gcgcagcagg tgtcgggctt gacgcaggat gacaggggga
     1261 aaccattatc aggtgcttcc gttgctttaa agaacgctaa ggattcctcc gtagtgaagt
     1321 taggaatttc gggctcatcc ggccaatatg aattttcacc ggtggccccc ggtcactact
     1381 tcgtaaccgt cacgcatatc ggctttgttc cgcaaaattc cgcctctttc ctgacaaaac
     1441 aggaaggcag gatacaaacg cccgccatat cgctcagccg catgccgaaa gaactgcggg
     1501 aagccgtggt gacaggccgg aaaccgctgg tggaagtaaa gcccgacaag atcgtcctca
     1561 acgtggaagg cagtatcaac gaagtgggct cggacgcact ggaactgtta cgcaaatccc
     1621 ccggcgtaac ggtcgacaag gacaataatc tgagcgtcaa cggcaggaac ggagtgcagg
     1681 tatacatcga cggtcgtccc acctatctct ccgtggcaaa ccttggcgac tacctcaaga
     1741 ccctgcagtc ctcttcgata gcatctatcg agatcatcag caatccttcc gcgaaatacg
     1801 aggccgcggg caatgccggc atcatcaata tccggctgaa gaagaaccag gcctacggga
     1861 cgaatatgac ggtctccgcc ggctacaaca tcggcacgta tagcaagtat aacggcgcct
     1921 tttcctttaa tcaccgcgat cagcggttca acatctttgg cgactactct tacaacaacg
     1981 cccgcaacga aggctattcc accctgttca ggacccagct ggacacgttt ttcctccaaa
     2041 gcaccacgct tgtcacgaca aacagcacac acaactataa agcggggatc gactatttca
     2101 tcgataagaa gagcaccctg gggttcatcg tcaacggcgt cagctccgac tatcatgtcc
     2161 gcagcaacag cgctaccccg atcgtctacc tgccgacaaa cggaacatcc aggatcctcc
     2221 aggccaacaa ccgcaccgat ggcagccgcg acaacctcaa ccttgacctg aactaccgct
     2281 acgccgacag cagcggtcac gaactgaaca tcgatgccga ctatggcctc taccatctcc
     2341 gcagcaacca gttccagcca aacgactatt tcgactccac ccgcaagtcg ctgctctata
     2401 gcaacgacta taatatgctg tcgccaagcg atatctatat ctatagcgtg aaggccgact
     2461 acgagttgcg ctgggcaaag gggatcctcg acatcgggct gaaaagctcc tatgtcacga
     2521 cccggaacaa tttccaggag tacaacatca ccaaccccac cggcggcggc acggaatccg
     2581 tgctggacac actcagcagc aacgatttta attataagga gaacatcaac gccctttacg
     2641 cgaattataa acgcacgctg aaaggctgga tcatccaggg cggcctccgc gcggagaaca
     2701 ccaacggcaa agggatctcg acaggctata aggaaagcgc cggcccctcc gatcctttcg
     2761 tgccctatga ctcttcgttc acccggcact ataccgatct tttccccagc gcctcggtca
     2821 cctggaacag ccatcccaca aaacaatgga cgctcagtta tagccggcgg atcgaccggc
     2881 cctcctacca gtccctcaac cccttcgagt tcaaactcga cgactacacg ttctcgcgtg
     2941 gcaacaccct gctgcgcccc cagtatacca acagcgtggg cctcacattc atgtaccact
     3001 acaaactcac cgccaacctc aactacagcc atacaaagga tctggccacc acgatcattg
     3061 acaccaccca gggatcgaag atgatcagcc ggcaggagaa cctggccacc caggatgtcc
     3121 tcagcctgac catcagttac cccttccagt acaaatggta tagcgccttc gtcaacatca
     3181 ccggtttcta ccagctcaac aaagccaatt tcggggccgg ccgcaccatc gatctgaacg
     3241 tcttcaatac caccgtctat acccagcaca ccctccgcct gggcagggga tggtcgggcg
     3301 agatcaccca atactacacc tcccccaatg tctggctggc caccctgcgc gccagttcgc
     3361 tttggaatat cgatgccggc ctccaaaaga ccatcttcgg cggcaacgga tcattcaagg
     3421 ttgccgtcac cgacatcttc cacacgctgc aatggactgc caccagcgat tttgccggcc
     3481 aggacatccg cgcctcggga ggctccgaga cccggatgct caaattgtat ctcacctacc
     3541 gcttcggcaa caaacacgta aaggccgccc gccggcatag caatggcgac gaagaagaaa
     3601 gcaaacgcgt aaagacccag tagccactgc ccccgcctct ctatacccgc ccgcacccgc
     3661 tgtcacatcc cctgccaatc cggcccccca tccccccgcc aaacgccccg gttttcctat
     3721 ctttgccggc tatgaccgcg atcaccgacg acatcagaac ccttttctcc ggtttttccc
     3781 cgaccccgct gacggagttg gagaagatcc cgcaatccgg cagcaaccgg atgtatttcc
     3841 gtatcctcac aggcaacggc tcctatatcg ccacctacaa cgataatatc cgggagaaca
     3901 gaacctttgt ccagtacagc cgccatttcc gtgcaaaggg ctgccccgta cccgagatct
     3961 actccatcaa tgacgcctat accatctatc tccaggaaga cttcggcgat cagtccctgc
     4021 tgaaagagct cgaaaaggag gggcataatg aacgggtcta cacctttttc cagaaaagcc
     4081 tcgaacaact ggcccgcctg cagatcctcg gggacgaagg gctggactat agctggacca
     4141 tcacgtcaag ggaattcggc aaacaggcca tcctcagcga cctgctctac ttcaaatatt
     4201 attttctcga caccctgcag atcccatacg ataaggaaaa gctgatcgac gacttcgagg
     4261 ccctgagcac ctatctcacc cacgtcgatc acaaatattt catgttccgc gactttcaga
     4321 gccgcaatat catgctgaag gacgaagcgg tccatttcat cgacttccag ggcggaatga
     4381 aaggcgcgtt acaatacgat gtcgcctccc ttctctggca ggcgaaggcc gagctacccg
     4441 acacctggaa ggataacctg ctcgactatt atatggactg cgtggaaagc atcctcgaca
     4501 cgaagatcga tcgtacccgg ttcatcagtc aatacaacgg ctatgtactg atccgtttgc
     4561 tccaggtact gggcgcctat ggcttccgcg ggctcttcga acggaaagcc catttcctca
     4621 tcagcattcc cctcgccctc cgcaatttca agtggttcct cggcaataaa caggtgggga
     4681 tcgttctgcc ggaattcgaa cggctgctgg gcctgatggt gcaggaggaa gtgatcagcc
     4741 ggttcgagcc gctgcaggcc gatgagaaca ccccgctggt cgtgcacatc aacagcttct
     4801 cctacaagaa ggcgcatccc gtcgatgaca gcggcaacgg cggcggtttt gtcttcgact
     4861 gccgcgggat cctcaacccg ggacggatac ccgagttcaa gacccagacc ggccgcgaca
     4921 agccggtcaa ggatttcctc gaacaacaga caaagatgcc cgaatttctg aatagcgtct
     4981 acgatctcgt cgacatctcc gtcgaagatt atatcaaaag aggattcggg agcctgcagg
     5041 tgagcttcgg atgtacaggg ggccaacacc ggagcgttta tgccgccgat gcactcgccc
     5101 ggcacctccg caacaaattc catgtcagga tccacctgca gcatatcgag caggaggcga
     5161 aaaattggat caactagttc tggtatgtac tatcttgtct acggctccct gtacctgctc
     5221 tccctgctgc cgatgtggct gctctatggt ctctcggacg gcatcgcctt cgtactctat
     5281 gcggtgatcc gctaccggcg cggagtggtc ctgtccaatc tgcgcatagc cttccccgaa
     5341 aagacggatg tccaacggct ggccatcgca aaacggttct accggaattt caccgataac
     5401 tttatcgaga ccatcaagct tctctccgcc ggagaggcct ggctgcagga acggttcgtc
     5461 ctcgacaacc ccgaactgct cgatcatttc tacgaccagg gacgcaaact gcaactccac
     5521 ctgggtcatc ttttcaactg ggagatcgca aatgtcgcga tacccacccg cacccgctat
     5581 acctttatcg tcgcctatat gccggtagag aacaaggcct tcgaacgcgt gttcctccgc
     5641 ctgcgcggcc gcacgggcac ggtcctcctg cccgcaacga agatgcagcg ggccatcctg
     5701 ccctaccgca atacgcagta catgctcgcc ctggtggccg accaggctcc cggcggaccg
     5761 gataattcct attggctgaa ttttttcggc cagcccacac cctttgtccg gggccccgaa
     5821 cggggcgccc ggatcgccga catcccggcc gtattcgtcc gcttttataa gccccgccgc
     5881 ggccactacc gggccgaact gatcaccatc gccgaccatc ccgccgcact tcccgaggga
     5941 gaactgaccc gccgctatcg ccggctgctc gaagaaggca tccgccagga accggccggc
     6001 tggctctgga gtcataagag atggaagttt acatggaccc cggggttcag gcctttttgg
     6061 atagatgaag aaagtttccc cccgaacgcc taaatagtta ccctgatcta tggcatatca
     6121 ggaaattata atacatttac tacaaccagt tcattacatc cttcaccgta cccggtccag
     6181 ggttaggatc gcctgatcag tagcacgacg accgatttct acccgcgtcg tcatcccgtt
     6241 cccctcaaat acaggactac ctaaacctaa acctaaaacc ggatgacaac ttttaacccg
     6301 ggggtggtac ttgtcgacga ccatgtacta ctccgcaacg gacttgcaag cctcatacgc
     6361 ggcttcggca ggttcgatgt tttattcgaa gcctctgacg gtaaagacct catcaggcaa
     6421 ttaagaattt cccgccgccc cgacatcatc ctgctcgaca tcggcatgcc cgaaatggac
     6481 ggctatgaga ccgcctgcta tctcaggcgt cattatcccg agatcagggt attggcattg
     6541 accatgtccg aaacggacag cgccatcgta cgcatgttga agaatggtgc acggggatat
     6601 atcctgaagg acatcgattc cgacggattg agacgggcgc tcgatgcgat ggtggagaaa
     6661 ggatattatt actcggaatt ggtgacggac aggctgatcg acaccatcaa ctatgtgggc
     6721 gaacccgaaa agcggatccg cgagattctc ctgctcaacg accgggagct cgagttcatg
     6781 aaactggtat gcacggagtg cacctacaaa gagatcgccg accggatgta cctcagccca
     6841 aggaccatcg atggctaccg ggatgcccta ttcgaaaaat taaacgtccg gacaagggta
     6901 ggccttgtat tatatgccat ccgcaatggg atcgtgagcg cctgagtccc gatccacc
//
LOCUS       DATGFG010000358         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_255170, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000358 DATGFG010000000
VERSION     DATGFG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255170"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(876..1121)
                     /locus_tag="VKQ52_06845"
     CDS             complement(876..1121)
                     /locus_tag="VKQ52_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86935.1"
                     /translation="MATEIKRIPVQDQGDAVTIINRLSLQYDMLPDTHRHQLHVANDR
                     YAAYYDAEEGVVKLEAIHPEVTKEEIEEFLMTFPPFF"
     gene            complement(1130..2068)
                     /locus_tag="VKQ52_06850"
     CDS             complement(1130..2068)
                     /locus_tag="VKQ52_06850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HLZ86936.1"
                     /translation="MKAIEIHHYGGPEELKLEEVPKPTINPDDVLVRVYATGVNPIDW
                     KIRKGGRSQSGTFPKILGWDFSGVIEQVGSQVTQWKAGDAVYGRPDPSRNGTYAEYVA
                     VKANEIARKPASIDHKAAAGVALAGLTAWQALFEHGQLQAGQRVLIHGAAGGVGIFAV
                     QFAKWKGAYVIGTSSEQNILFLEELGADEVIDYRKEDFSRVVADIDLVVDTIGGKVQE
                     DSMKVLRQGGTLVSTVGIQDPEALKARGIRGVAFMAQSGTADLEQIASLIDSGAVIPV
                     VSKILPLKDAAEAQRLSEEGHTRGKIVLQVVEDGTN"
     gene            2463..2933
                     /locus_tag="VKQ52_06855"
     CDS             2463..2933
                     /locus_tag="VKQ52_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86937.1"
                     /translation="MKLFVAGLPSDFDDVDLKEMFELYGTVNFAKVVMDRETGKSKGF
                     GFVDMPDDTEARGAIETLDNASIRGKKMAVKEADEQNRGGGDRGGGGDRGGDRGGGGG
                     YRGGGDRGGGGGYRGGGDRGGGGGGYRGGGDRGDRGGGGGGYRGGGDRGPGRRY"
BASE COUNT          667 a          920 c          793 g          744 t
ORIGIN      
        1 cgctcgatgc gccaggtgta ctactatgcc gtccgcgccg tctcccccgg tgtctaccat
       61 atggggcccg tcagcgccga cgccatgtac aatggtgaat atcattcgta caacggcgcg
      121 ggcaccatcc gggtgacgca ataatgatct tcatggccgg gtcatctcag cggagggatg
      181 atccggtctt ttttatcaac tcttcttatc ttactaaaac gttttttcga tgggactatt
      241 tagctcatgg tttggcaaga aaaagcagga gccaaccgcg ccgccgcaac cagcaacacc
      301 gccgcgatcg accgcgccgc aacccgacag cccgcgccgg ccggaaacgc cgcaaccacc
      361 agccacaccg ccgcctccgg aggccatgga aaaagtagcg gtccttcccg gtatcgaact
      421 ctcaaaaccc cttgccgcct gctggccaca gatcgccgcc acgaaacgga cctctataaa
      481 aatcacggcc acccccgtcg acgaccccca ttgccggaaa aactcctttg gctgttatcc
      541 gacccttcca aagggcttcg actatccaaa ggatgagaag gggaactata tgtacccact
      601 cgcccagatc cactgcgccg acctgcctcc cctagccggc tatcccgaca gcggctatct
      661 gcaattttat attgccgtcg aagacaacga catctatggc ctggatttcg atgataaggt
      721 gatccaaaaa aatttccggg tgctttactt cggagaaaat gaggttccga tgacgagatc
      781 atgtggggcg atgtcggagt ggccaacttt ttcatcgacg ccgaccgcct gcgaaagaag
      841 gacttctcca ccgttttcta cacctgggat tgtagctaga aaaagggcgg aaaggtcatc
      901 aggaattcct cgatctcctc tttcgtcacc tccgggtgga tcgcctccag tttgaccacc
      961 ccctcctccg catcgtaata ggccgcatac cggtcgttgg ctacatgcaa ctgatgccgg
     1021 tgcgtgtccg gcaacatatc gtattgcagg ctgagccggt tgatgatcgt cactgcatca
     1081 ccctgatcct gaacggggat gcgtttaatt tctgtcgcca tataaaggtt tagtttgttc
     1141 catcctccac cacctgcagc acgatctttc cgcgcgtatg cccctcctcg ctcaggcgct
     1201 gcgcctcggc tgcatctttc aacggcaaga tcttcgagac tacggggatc accgctccgc
     1261 tatcgataag ggaagcgatc tgttccaggt cggccgttcc cgactgcgcc atgaacgcaa
     1321 cgccccggat acccctggct tttaacgctt cggggtcctg gatccccacc gtactcacca
     1381 gtgttccgcc ctggcgtaat accttcatgg aatcttcctg caccttgccg ccgatggtat
     1441 cgacaaccag gtcgatatcc gcaacgaccc ggctgaaatc ttctttcctg taatcgatca
     1501 cttcatcggc gcccaattcc tcgaggaaga gtatattctg ttccgaagac gtgccgatca
     1561 cgtaagcgcc cttccatttg gcaaattgca cggcaaaaat acctacgcca ccggccgctc
     1621 cgtggatcaa tacgcgttgc cccgcttgca gttgtccgtg ttcgaacagg gcctgccagg
     1681 cggtaagccc ggccagcgct acacccgccg ctgccttatg gtcgatggag gcgggcttcc
     1741 gcgcaatctc attcgccttc accgccacat attcggcata cgtgccgttt cgcgagggat
     1801 caggccggcc atacacggca tctcctgctt tccactgggt tacctggctg ccgacctgct
     1861 cgatcacccc tgaaaaatcc cagccgagga tcttcggaaa agttccgctc tggctacggc
     1921 cgcccttcct gatcttccaa tcgatcgggt tgacgccggt tgcatacacc cggaccagca
     1981 catcgtccgg gttgatggtc ggcttgggca cttcttccag tttcaactcc tccggaccac
     2041 catagtgatg tatttctatt gctttcatga tcagtcaatt tgtttgaatt actattggga
     2101 atatgcaaat cccgcgcctc ctactatccg caccgcaccc taatgcacca tcactcacca
     2161 tcacgcaccc gtcgcgcacc catcctgcgc accggaataa atattccttc taccccttcc
     2221 cgtttaactt aaggggtttc ttttcccgtc tgctatgggt aaaaagctac ctcccgattt
     2281 attgttaggg aatgttcgcg taaaacttta tttttatcgg gagattctct tcttttgttg
     2341 ggagattcac tgcgtgtttt tactatcgcc gggtctaaca taaaaattag cggaacttat
     2401 tgtgagataa attagaatat ataattttta tctttgtaat tttactcttt aaatctatcg
     2461 gcatgaagct ttttgtagca ggtcttcctt cagactttga tgacgtagac ctgaaagaaa
     2521 tgttcgaatt atatggcaca gtgaattttg caaaggtggt gatggatcgc gagacaggta
     2581 agagcaaagg cttcggattc gtggatatgc ccgacgatac ggaggcgcgt ggagcaatcg
     2641 aaacattgga caacgcctcc atccgtggaa agaagatggc ggtaaaagaa gccgatgaac
     2701 aaaatcgtgg cggcggtgat cgcggcggcg gtggggatag aggtggtgac cgcggcggtg
     2761 gcggcggtta tcgtggtggc ggcgaccgcg gcggcggtgg tggttatcgt ggcggaggtg
     2821 atcgtggcgg tggcggcggc ggctatcgcg gtggtggtga tcgcggcgac cgtggtggtg
     2881 gtggcggcgg ttatcgcggc ggcggcgatc gcggtcccgg cagaagatat taaaacggaa
     2941 acaacaacac aaagaataag accgggaact tctcccggtc ttcgtttttt agagggcgtg
     3001 ctagacattt ctccagtgtc cggggatcca tctccatccc ccgcgggttt cggcccaatg
     3061 acctccttcc cacacagccc tttcacggcg tctttcccag tgcccttcat gccattcata
     3121 ccgg
//
LOCUS       DATGFG010000359         4537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1405391, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000359 DATGFG010000000
VERSION     DATGFG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405391"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VKQ52_06860"
     CDS             <1..325
                     /locus_tag="VKQ52_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86938.1"
                     /translation="QQSVDNIYFNAYGFGPNSETVWDGTVIRLREVSLSYSVPSGLLK
                     KSPFGAASINLSGTNIWYLAPNVPKYTRFDPEVNSLGVTNAKGIDLFAGPSSRRFGAS
                     LRLTF"
     gene            344..2029
                     /locus_tag="VKQ52_06865"
     CDS             344..2029
                     /locus_tag="VKQ52_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ86939.1"
                     /translation="MIPYKYSLIIVLSAAALLSGSCNKQLSPLLNNPSTASPSAADPD
                     LYLNNLQLNFKGFFQDASDLSDQLVRQETMFGPTYYNAFAPTSFDDIWTKAYESVFLT
                     ANTMIPIAEGKQEYVHAGIAQVLKAYTMITMVDLFGDVPYSQADLGVGNTNPKVDKGA
                     DIYDSALALLDSGIANFARKPSVGPANDLFYGGSATNWRKLAKTLKLKAYIQTRLVDN
                     SVAAKATALINENDLVNSTSQDFVFSYSTHNQAPDSRTSHYVTNYGTDNGAGDYMGNY
                     FLWCLRDEKTIPDPRTPYYIYRQIDDITNDSRLPDRTTTQFALACYYRSDPYPAGVAY
                     CIIDNGYWGRDHGNNEGIGPDNSLRSTWGLYPAGGAFDANQNKATGQSTGRITGAGGN
                     GINPIWLSSYTYFLKAEAALILGAPGDPRALTVSGVDQSFNKVAGFASSIGYTLPTTD
                     TTFLITPTKQQQYESVVQNLYDNASTTAAKLNVVEKEYYLAAWGNGVEPYNNYRRTGC
                     PNNMQPALYGNPGLFIRSFFYASVYANYNKNAVQKTVTNVKVFWDTNSDNFVY"
     gene            2042..2920
                     /locus_tag="VKQ52_06870"
     CDS             2042..2920
                     /locus_tag="VKQ52_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86940.1"
                     /translation="MKKYIKASLCVGIICLLIHACSKKNISGDSSNLVLGSYITLDST
                     INNILDVGNSSSTVSIMVGKYVGNPVTTINIFVATGSNKEDTTGWKFIKSVAYSNGAV
                     LSVSTAELAKALGANPAPGNKYTLQNQVVTSDGRKFSTFNTPDTYNSFPAYNMALTWY
                     ATAVCAFTGNMAGNYNLTKDVWNDYGASPSNPVLIPGAVKDGPGANQISIYVYPGAQA
                     GGVETGQMIVSVNPVTGAATIVKSDIGNYGSPSSETIVTGSGFVFDCVGVIDLNITFT
                     YGGTPYANQEMILQKQ"
     gene            complement(3033..>4537)
                     /locus_tag="VKQ52_06875"
     CDS             complement(3033..>4537)
                     /locus_tag="VKQ52_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HLZ86941.1"
                     /translation="EDKKLVELHHEKADASFAVGDLYLGKVKKLIPGLNAAFVDVGFE
                     KDAFLHYTDLSPYARSLLKFTQAAMNDTVPGGFDFGTFQVEPEIIKTGKINEVLNGKP
                     HILVQILKEPIAAKGPRLSCEISLPGRFVVITPFNDIVAVSRKIHSSDERKRLQKIVE
                     AIKPKNFGVIVRTAAEGKNTAELHEDLTELVATWQTIKRNLKGAVAPAKILSEQTKTT
                     SMLRDLLNEDFNKIVINDRNIFNDTKSYIQRIAPDKIDIVSYYHNGSPIFDQFSVTKQ
                     VKAAFGKTVNLPSGAYLIIEHTEALHVIDVNSGYKSVSNNQEQNALETNMEAAEEIAR
                     QLRLRDLGGIIVVDFIDMKLPDNKRKLIEAMEGFMRTDRAKHAVLPISKFGLMQITRQ
                     RMKPEVTINTQEVCPTCNGTGKISSALILEDEIEKNLSYLLNHQHKDLILAVHPIMHA
                     YLTKGGFLGIGSRQWNWRWKYKQKVRIRANTNYHLTEFHFFDKQEDEIKL"
BASE COUNT         1103 a         1310 c         1066 g         1058 t
ORIGIN      
        1 ccagcagagc gtcgacaata tctatttcaa cgcttatggg tttggcccca attcagagac
       61 agtatgggac ggcaccgtta tccgtttgcg cgaagtatcg ctgtcgtata gcgtcccttc
      121 cggcctgttg aagaaaagtc ctttcggcgc tgcctctatc aacctttccg gcacgaatat
      181 atggtacctg gcaccgaatg ttccgaaata caccaggttc gatcctgagg tcaacagtct
      241 cggtgtcacc aacgcaaagg gcatcgacct gttcgccgga ccctcttcac gtcgtttcgg
      301 ggccagcctg agattaactt tctaatccaa aaaacgaaat agtatgattc cgtataaata
      361 cagtcttata atcgttcttt ccgctgcagc cctgctatcc gggtcttgca ataagcaact
      421 cagccccctg ctgaataacc caagcacagc ttccccttcg gctgcagacc cggacctcta
      481 tctcaataat ctgcaactga atttcaaggg atttttccag gatgcctccg acttgtctga
      541 tcaattggtg cgccaggaga ccatgtttgg acccacctat tataatgcct tcgcacccac
      601 cagcttcgat gatatctgga caaaggccta cgaatcggtc ttcctgacgg caaataccat
      661 gatacctatt gcggagggca agcaggaata tgtccacgcg ggtattgcac aggtgctgaa
      721 agcctatacc atgatcacga tggtcgacct tttcggggac gtaccctata gccaggccga
      781 cctgggcgta ggcaacacca acccgaaggt ggataaaggt gcggatatct atgactcggc
      841 cctggcgttg ctggattcag gcatcgccaa tttcgccaga aagccatccg tcggcccggc
      901 caatgatctg ttctatggcg gtagcgccac aaactggagg aagctggcca agacgctcaa
      961 attgaaggcg tatatccaaa cccgcctggt tgataactca gtcgccgcta aggctacggc
     1021 cctgatcaat gagaacgacc tcgtcaattc aaccagccag gatttcgtct tttcctattc
     1081 tacccataac caggccccgg atagcaggac atctcattat gtgaccaatt atggcacgga
     1141 caacggcgcc ggcgactata tgggcaacta ttttttgtgg tgcctgcggg atgagaaaac
     1201 catacccgat ccaaggacgc cctattatat ctatcgccag atcgacgata ttacaaatga
     1261 ctcccgcctt cctgaccgga cgaccaccca attcgccctc gcctgctatt accggtcaga
     1321 cccttacccc gctggagttg cctattgcat catcgacaac ggctactggg gaagagacca
     1381 tgggaataac gagggcatcg gacccgacaa ttcgctccgg tccacctggg gattataccc
     1441 tgcgggcggg gcatttgatg ccaatcagaa taaagccacc gggcaaagca ccgggcgtat
     1501 caccggggcc ggcggcaacg gtatcaatcc catctggctg tcttcttata cctatttcct
     1561 gaaggcagaa gccgcactga tcctgggtgc acccggagat ccgcgcgccc tcaccgtgtc
     1621 gggagtcgac cagtccttca acaaagtagc aggcttcgca tcaagcatcg gctatacact
     1681 gccgacaacg gataccacct tcctcatcac gccgaccaaa cagcagcaat atgagtccgt
     1741 tgtacaaaac ctgtatgaca atgccagcac cactgccgcc aaactgaatg tggtcgagaa
     1801 ggagtattat ctcgctgcct ggggaaatgg ggtcgagccg tataacaatt atcgcagaac
     1861 cggctgcccg aacaatatgc aacccgcctt atacggtaac cccggcctgt tcatccggtc
     1921 attcttttac gcttccgtgt atgccaatta caataagaac gccgtgcaga agacagtcac
     1981 caacgtaaag gtattctggg ataccaactc ggacaatttt gtttattaaa aaatgaaggt
     2041 catgaaaaaa tacatcaaag catctctgtg tgtcggtata atatgcctcc tgatccatgc
     2101 gtgctccaaa aagaacatca gcggcgattc ctccaacctg gtgctgggct cctatatcac
     2161 cctggattcc accatcaaca atatcctgga cgtcggcaat tccagcagca ccgtttccat
     2221 catggtgggc aaatacgtag gcaaccccgt gaccaccatc aatatcttcg ttgccaccgg
     2281 ctccaataaa gaggatacca ccggttggaa attcatcaaa tcggtggcct attcaaacgg
     2341 cgcggtcctg agcgtttcta ccgccgaact ggccaaagcc ctgggcgcca acccggcgcc
     2401 aggcaacaag tataccctgc aaaaccaggt ggtcacgtcg gatggccgga aattctccac
     2461 cttcaatacg cctgacacgt acaatagctt tcccgcctat aatatggcgc tcacctggta
     2521 cgccacagcc gtctgcgctt ttacaggcaa catggcgggg aactataatc tcacaaaaga
     2581 cgtttggaat gattatggag caagtccatc gaaccctgtg ctgatcccgg gtgcggtaaa
     2641 agacggtccg ggggctaacc agatctccat ctatgtttat cccggcgcac aggcgggcgg
     2701 cgtcgaaaca ggtcagatga tcgtcagcgt gaacccggtc accggagcag cgacgatcgt
     2761 aaagtccgac atcggaaact acggcagccc ctcgagcgag accatcgtta ccggcagcgg
     2821 gttcgtattc gactgtgtag gcgtaattga tctcaacatt acgttcacct atggcggtac
     2881 accctacgcc aaccaggaaa tgatactgca gaagcaataa aacataggcc cctgcctgaa
     2941 ttctgtgtga ctcaatgttg acagtgaaag acggctccgg tttctaccgg ggcctattct
     3001 tttttcgccc gcgattcatc gtgaacgaaa aactacaact tgatctcgtc ttcctgcttg
     3061 tcgaagaaat gaaattcggt gagatggtaa ttcgtattgg cgcggatccg caccttctgc
     3121 ttgtacttcc agcgccagtt ccactggcgg ctgccgatac ccaaaaaacc acccttggta
     3181 agataggcat gcatgatggg atgcacggca aggatcaggt ccttgtgctg gtgattgagc
     3241 aggtaactaa ggttcttctc gatctcatcc tccaggatca gcgcggacga gatcttgccc
     3301 gtaccattac aggtaggaca aacctcctgg gtattgatcg ttacctcggg cttcatccgc
     3361 tgacgcgtga tctgcatcag cccgaacttg gaaataggaa ggaccgcatg cttggccctg
     3421 tccgtccgca taaaaccttc catggcctcg atcagcttcc gcttgttgtc cggaagcttc
     3481 atatcgatga aatccacgac gataatgccg ccaagatccc gcagccgcaa ttgtcgcgcg
     3541 atctcctcgg ccgcttccat attcgtctcg agggcattct gctcctggtt attgctgacg
     3601 ctcttatacc cgctgttcac atcgatgaca tgcagcgcct ccgtatgctc gataattaaa
     3661 taggctccgc ttggaaggtt aaccgtcttg ccaaaagcgg cctttacctg tttggtcaca
     3721 ctgaattggt cgaatatggg actgccattg tgataatacg atacgatatc gatcttatcc
     3781 ggggcgatcc gctggatata actcttggtg tcgttaaaaa tattccggtc gttgatgact
     3841 atcttattaa aatcctcatt caacagatcc cgcaacatac tggtcgtctt cgtctgctca
     3901 ctgaggatct tcgccggcgc aaccgccccc ttcaggtttc gcttgatcgt ctgccaggtg
     3961 gccaccagct ccgtcaggtc ctcatgcaac tccgccgtgt tcttaccttc cgcggccgtg
     4021 cgcacgatca caccgaaatt cttgggtttg atggcctcca cgatcttttg cagccgcttc
     4081 cgctcatccg aagaatggat cttccgcgac acggcaacaa tatcattgaa gggagtgata
     4141 acgacaaacc gccccggcaa agagatctca cagctaagcc gcggaccctt tgcagcgatc
     4201 ggttctttta agatctggac aagaatatgt ggcttgccgt tcagtacctc attgatcttc
     4261 cccgtcttga tgatctccgg ctccacctgg aaggttccaa agtcgaaacc acccggcacc
     4321 gtgtcattca tcgccgcctg cgtgaacttc agcagactcc gggcataggg actaagatcg
     4381 gtataatgga gaaaagcgtc tttttcgaaa cctacatcca caaaggcagc attcagaccc
     4441 gggatcagct tcttcacctt ccccaggtat aaatctccaa ccgcaaagct ggcatcagcc
     4501 ttttcgtggt gcagctctac cagcttcttg tcttcca
//
LOCUS       DATGFG010000360         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1625553, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000360 DATGFG010000000
VERSION     DATGFG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625553"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2871)
                     /locus_tag="VKQ52_06880"
     CDS             complement(<1..2871)
                     /locus_tag="VKQ52_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ86942.1"
                     /translation="MKLILFALRKPITILVVVAALFFFGLGALRSIRVDIFPKLDLPV
                     LYIAHPFGGYTPEQMETLFAKQYINVLLFVNGIKSIETKNVQGLTLMKLNFYPGTNMA
                     QAAAEVSAVSNRAQVLFPPGSNPPFIIRFDASTLPVGQLVMSSDKRSNNELLDLANLY
                     VRSSFTSIPGLLSAPPFGGNIRTVVIKADPELLRAHNLTADQIVQAIDVNNQMSPSGN
                     VRIGDKNYITPVNTTIREVKDFGNIPIFKGGVQNLYIRDVAAVEDGADVTVGYGLVNG
                     KRSVYLSIAKNADASTWEVVQKLKAAIPRMQAQLPEDVKLSYEFDQSVYVINSVRSLI
                     SEGAIGALLTGLMVVLFLGDLRGALIVIMTIPTSIISGVLFLNLFGQTINIMTLSGLA
                     LAIGILVDESTVTIENIHQHLDMGKPKRLAIWDACKEIAFPKLLILFCILAVFAPAFT
                     MGGIPGSLFLPLSLAIGFSMIVSYFLAQTFVPVMANWIMKAHAPHDGEGKPGRFDRVR
                     ARFLRFVDRMMPYRRSVVMAYLLLTSVAAVILLRGIGKDVLPKVNGSQFQVRLRAPEG
                     TRIERTEEKTLKTIAIIKDIVGPENVDITSAYVGQHPSLFSVNPIYLWMAGPQEAVLQ
                     VGLNEHYKTNLDALKEKIRTRVDSALPDINLSFEPIELTDKILSQGSPTPVEVRFSGR
                     NKKNNEIYALKLIDRLKAISYLRDVQLGQSTRYPAIDIDVDRVRAAQLGVDINDVARS
                     LTASTSSSRYTDKNIWVDQKVGNSYSVQVEVPENKMKSVGDIGEIPLLRNATRPVLGD
                     VATIKEDTTYGESDNLGAIPILSVTANLNGRDLGAATRDIRAAIASLGPLPRGLTVEP
                     IGMSQTLVDTLDSLQNGLLVAIVVIFLMLAANFQSFKVSAIVLATVPAVILGSLLLLL
                     ATGSTLNLQSYMGIIMSVGVSISNAVLLITNAEQLRKHNGSAL"
     gene            complement(3013..3567)
                     /locus_tag="VKQ52_06885"
     CDS             complement(3013..3567)
                     /locus_tag="VKQ52_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ86943.1"
                     /translation="MRESAVKDRILDTASRLFYDQGYHVTGINQIIEEADIARASLYN
                     HYPSKTDLLLAYLDRTHEEWFRELDLYLAPLASPREKLLALFDYRIQRQQKLKYKGCH
                     FNKITAETSDDEQVARRVKMHKERFRQYILHLVKQTDHRRLLDNDTLTDNLFLLLEGG
                     IAVGAMFRSSEYAEKARRIAESFL"
     gene            3741..>4224
                     /locus_tag="VKQ52_06890"
     CDS             3741..>4224
                     /locus_tag="VKQ52_06890"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLZ86944.1"
                     /translation="MKKRLAILWLVLLASGIAIVFWRYEWKYSLPTPIPERYHAVGLG
                     SAITLPASAAVLLPADRKPLFLHFFNPDCPCSRFNMPQFRALVQQYGKEATFAIVIMS
                     PTRFTARDIQTKFDLPYPIPVISDSAVAAACGVYSTPQAVIIDKNRQLFYRGNYNRSR
                     Y"
BASE COUNT          978 a         1208 c         1111 g          927 t
ORIGIN      
        1 cagggcgctg ccattatgct ttcgcaactg ttcggcattg gttatcagca agacggcatt
       61 ggagatggac acccccaccg acatgatgat ccccatatac gattgcaggt ttagcgtaga
      121 cccggtagcc agcaacagca acagcgaacc caggatcacg gcggggacgg tagcaagaac
      181 aatggcgctc accttaaagg attgaaaatt cgcagccagc ataagaaaga tcacaacgat
      241 cgccaccagc aacccgttct gcaggctgtc cagtgtatcg accagggtct ggctcatccc
      301 gatgggttcc accgtgagcc cccggggcag cggccccagc gaagcaatgg ccgcccggat
      361 atcccgcgta gcggccccca ggtctctccc gttgagatta gcggttaccg aaagaatggg
      421 gatggcgccg aggttatcgc tctccccata agtggtatcc tcctttattg tcgccacgtc
      481 gccaagcacg ggccgcgtgg cattccgcaa caaagggatc tcgccgatat cacccaccga
      541 cttcatcttg ttttccggga cttccacctg tacgctgtaa ctgttcccca ctttttgatc
      601 gacccagata ttcttatcgg tgtaccggga agaagaggtg gaggcggtga gcgaacgcgc
      661 cacatcgttg atgtccacgc ccagttgtgc cgcacgcacc cgatcgacat cgatatcgat
      721 cgccgggtaa cgggtcgact gccccagctg tacatcccgg agataggaga tcgctttgag
      781 ccggtcgatc agcttcagcg catagatctc gttgttcttt ttattccggc ccgagaaacg
      841 cacttccacc ggcgtcggcg agccctggct gaggatcttg tccgtcaatt cgatgggctc
      901 gaaagacagg ttgatatcgg gaagggcgct gtccaccctc gtcctgatct tctcttttag
      961 cgcgtccagg ttggtcttgt agtgttcgtt caaccctacc tgtaacaccg cctcctgcgg
     1021 accggccatc cagagataga tcgggttgac ggagaacagg gaagggtgtt gccctacata
     1081 ggccgaagta atgtcgacat tttccgggcc gacgatgtct tttatgatcg cgatcgtctt
     1141 cagcgtcttc tcttccgtgc gttcgatccg ggtcccttcg ggcgcacgga gccgcacctg
     1201 gaactggctg ccgttcacct tgggaagcac gtccttcccg ataccgcgca gcaggatgac
     1261 cgcggccaca ctggtcaaca gcaggtaggc catgacgact gaccgcctgt aaggcatcat
     1321 ccggtcgaca aaccgcagga aacgggcgcg taccctgtcg aaacgaccgg gcttgccctc
     1381 cccgtcgtgc ggcgcatgcg ccttcatgat ccagttggcc atcacgggca caaaggtctg
     1441 ggcgagaaaa taggaaacga tcatgctgaa tccgatggcc agcgacaagg gcaggaacag
     1501 cgagcccggg atgccgccca tcgtaaaggc cggagcgaag accgcgagga tacaaaacag
     1561 gatcagcagc ttgggaaaag cgatctcctt acacgcatcc catatcgcca accgcttggg
     1621 tttccccata tccaggtgct ggtggatatt ctcgatggtc accgtactct catccaccag
     1681 gataccgatg gccagcgcaa gcccgctcag ggtcatgata ttgatggtct ggccgaacag
     1741 gttcaggaag agcacgccgg aaatgatgga ggtagggatc gtcatgatca cgatcagcgc
     1801 cccccgcaga tcacccagga agagcacgac catcagcccg gtgagcagcg caccgatggc
     1861 gccttccgaa ataagactcc tgacggagtt gatcacatac accgactgat cgaactcata
     1921 ggacagcttt acatcttccg gtagctgtgc ctgcatccgg gggatcgccg ccttcaattt
     1981 ctgtacaacc tcccaggtgg aagcatcggc attcttggcg atgctcaggt aaacactccg
     2041 cttgccgttg accagcccat accccaccgt gacatccgcg ccgtcttcta cggccgctac
     2101 atcacggata tagagatttt gcaccccgcc tttgaagatc ggaatattcc caaagtcctt
     2161 tacctcccgg atggtcgtat tcaccggcgt gatatagttc ttgtcgccga tgcggacatt
     2221 accggatggc gacatctggt tgttgacatc aatcgcctgc acgatctggt ccgccgtaag
     2281 attatgcgcg cgcaacagtt cgggatcggc cttgatgacc accgtgcgga tattgcctcc
     2341 gaagggcggc gccgacagca atcccgggat agaagtgaac gaactgcgga catagaggtt
     2401 ggccagatcg agcaattcgt tattgcttcg tttgtcgctg ctcatcacca actgaccgac
     2461 gggcagggtc gaggcgtcga aacggatgat aaagggggga ttggacccgg gtgggaaaag
     2521 cacctgggcg cggttgctca ccgcgctgac ctcggccgcc gcctgcgcca tattggtgcc
     2581 gggataaaag ttcagcttca tcagggtaag cccctgtacg ttcttggtct cgatgctctt
     2641 gatgccgttc acgaacagca acacatttat atactgtttg gcaaacaggg tctccatctg
     2701 ttccggagta tagccgccaa aagggtgcgc gatatacagg accgggagat ccagcttagg
     2761 aaaaatgtcc acccggatgg aacgcagggc gccgaggcca aagaaaaaga gggccgcaac
     2821 cactaccaaa atagtgattg gttttcgcaa agcgaagaga atcaatttca tacaccttaa
     2881 ttaaaaagag agaaagcggg ttattttcca cggagcaggc aaccggttga gcagacaagt
     2941 ctatctaaaa aatttgaagc aaatttgaac ctcgatgcgg ttccttattt gacttccggc
     3001 tagatggctc ccttagagga aggattcggc gatccgcctg gccttctccg catactccga
     3061 actgcggaac attgccccaa cggcgattcc cccttccagc aacaggaaca ggttatccgt
     3121 caaggtatcg ttatccagga gacggcgatg gtcggtttgt tttaccaggt gaagaatata
     3181 ctgccggaac cgttccttat gcatcttcac ccgccgtgca acctgctcat cgtcactggt
     3241 ctcagccgtg atcttattaa agtgacagcc cttgtatttc agcttttgct gtcgttggat
     3301 acggtaatcg aacaaagcaa gcagcttctc ccttggagaa gccaacgggg cgagatagag
     3361 atccagttca cggaaccatt cctcatgcgt cctgtccagg taagccagca ggagatcggt
     3421 cttggaagga taatgattgt aaagggatgc ccgggcgata tccgcctcct cgatgatctg
     3481 gttgatcccg gtcacatggt atccctggtc gtagaacagc cgcgaagccg tatccaggat
     3541 cctgtctttt actgccgact cccgcatgga acaaaaatag gagttttatg caaaacagac
     3601 aactctgtct attttagcaa aaataaataa ttccccctaa taagggcttc cacctaataa
     3661 aatgggcccg atctgcgtta ttgatcagga cgggtatacc cctatagata ccatccattc
     3721 ctatgacatt atccaacagt atgaaaaaga gattagccat cctttggctg gtcttgctgg
     3781 cctccggcat tgccatcgtt ttctggcgat atgaatggaa atacagtttg cccaccccca
     3841 taccggagcg ttaccatgcc gtagggttgg gcagtgccat tacgcttccc gcttcggcag
     3901 cggtcctgtt gcccgcggac cgaaaaccct tgttcctcca cttcttcaat cccgattgtc
     3961 cctgttcgcg tttcaatatg ccccagttca gggccctggt acagcaatat gggaaagagg
     4021 ccacgttcgc tatcgtcatt atgagcccaa cacggttcac tgcccgggat atccagacga
     4081 aattcgatct gccctacccg atacccgtga tcagcgatag cgcagtggcc gcagcctgtg
     4141 gcgtctattc caccccccag gccgttatca tcgataagaa ccggcaattg ttctaccggg
     4201 ggaattacaa ccgcagccgt tatt
//
LOCUS       DATGFG010000361         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_939928, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000361 DATGFG010000000
VERSION     DATGFG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939928"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VKQ52_06895"
     CDS             <1..558
                     /locus_tag="VKQ52_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLZ86945.1"
                     /translation="LSSIDHYQVEVMITVALVMGGYMLADRLHVSGPIAMVVAGLITG
                     NQSRMAAMSETTRDYLDKFWEMIDEFLNAILFLLMGFELLVLPFDRVYVVMGLICIPI
                     MLIARYLSVLLPISFLQYHTAFERNAIPILTWGGLRGGISVALALSIPTDMHGELFVT
                     VTYIVVLFSILVQGLTIGKIAKRLA"
     gene            complement(555..2024)
                     /locus_tag="VKQ52_06900"
     CDS             complement(555..2024)
                     /locus_tag="VKQ52_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HLZ86946.1"
                     /translation="MGKPTGFLEFDRQLPSKRPVHERKNDYKEFVNTYDGAQLNHQAA
                     RCMNCGIPFCHSGCPLGNVIPEFNDAVYRQQWQEAYEILTSTNNFPEFTGRICPAPCE
                     SSCVLGINQPPVAIEEIERHIIEIAFDKGFVKANHPALRTGKKVAVVGSGPAGLAAAD
                     QLNAAGHSVTVFERDDQPGGLLRYGIPDFKLEKWVVARRVKLMEEAGIVFRCNAEVGV
                     NISINDLLREFHAIVLAGGSTIPRDLPIPGRQLKGVHFAMDFLKQQNKRVSHLPVDGE
                     DLLATGKNVVVIGGGDTGSDCVGTSNRHKAISVTQFELMPKPPHSRTEFMPWPTYPMV
                     LKTSSSHEEGADRHWAIATKEFIGDDQGNLKALKTVKLEWKTTADGKAAQFTEVPGSE
                     QELPCELALLAMGFVHPQHKGLLKELDIELDERGNVKASDRNYQTSIAKIFTAGDMRR
                     GQSLVVWAISEGRECARKVDEYLIGTSLLERKDQSLIAI"
     gene            complement(2137..>2762)
                     /locus_tag="VKQ52_06905"
     CDS             complement(2137..>2762)
                     /locus_tag="VKQ52_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86947.1"
                     /translation="AGQSFGAFNTHGVVLELEGDANDYFGKGLSGARLIVYPSQNAGF
                     VPEENSIIGNVAFYGATSGEAFIRGKAGERFAVRNSGVKAVVEGVGDHGCEYMTAGRI
                     VILGNTGRNFAAGMSGGIAYVYDVRGTFGRNVNREMVDLDPVAGEDISELRDMIQRHY
                     AYTGSNVAKFVLDDFENQLQHFVKVFPTDYKKVLLEKAARPQRTTHK"
BASE COUNT          470 a          671 c          927 g          694 t
ORIGIN      
        1 ctgagcagca tcgaccatta ccaggtggag gtgatgatca cggtggcgct ggtgatggga
       61 gggtatatgt tagcggacag gttgcatgtc agcgggccga tcgcgatggt ggtggcgggg
      121 ctgatcacgg gcaaccagag ccggatggcg gcgatgagcg agaccacgcg ggattacctg
      181 gacaagtttt gggagatgat cgatgagttc ctgaacgcca tcctgttcct gctgatgggg
      241 ttcgagctgc tggttttgcc ttttgacagg gtgtatgtcg tgatggggtt gatctgtatc
      301 ccgataatgc ttatagcgcg ttacctgtcg gttcttttac ccatcagttt cttacaatac
      361 catacggcat ttgagcggaa tgcgataccc attctcacct ggggcgggtt gcggggaggc
      421 atatccgtgg cattggctct ttccattccc acggatatgc atggtgagtt gtttgtcacc
      481 gttacctaca ttgtcgtcct cttttctatc ctcgtacagg ggctgacgat cgggaagatc
      541 gcaaagcgac tggcttagat agcgatcagg ctttggtcct tgcgttcgag cagggaggtg
      601 ccgatgaggt attcgtcgac cttgcgggcg cattcgcggc cttcgctgat ggcccagacc
      661 acgagggact ggccgcggcg catgtcgccg gcggtgaaga tctttgcgat gcttgtctgg
      721 tagttgcgat cggaggcttt tacgttgccg cgctcgtcga gttcgatgtc gagttctttc
      781 aggaggcctt tatgctgggg atggacgaag cccatggcga gcagggccag ttcacacggc
      841 agttcctgtt cgcttcccgg cacttcggtg aactgggcgg ccttgccgtc cgcagtagtc
      901 ttccattcga gctttactgt tttgagcgct ttgaggttgc cctggtcgtc gccgatgaat
      961 tcctttgtgg cgatggccca gtggcgatcg gcgccttctt cgtgggaaga ggaggtcttg
     1021 aggaccatgg gataggtggg ccagggcatg aattcggtgc ggctgtgggg tggttttggc
     1081 atcagttcga actgggtgac cgagatggcc ttgtgccggt tggaagtacc tacgcagtcg
     1141 ctgccggtgt cgccgccgcc gatgacgacg acgttcttgc ccgtggcgag gaggtcttcg
     1201 ccgtccaccg ggagatggct gacgcgtttg ttctgttgtt tgaggaagtc catggcgaag
     1261 tgtacgcctt tgagctgccg gcccgggatg ggcaggtcgc gggggatggt ggagcccccg
     1321 gccaggacga tggcgtggaa ttcgcggagg agatcgttta tgctgatgtt gacccctact
     1381 tctgcattgc atctgaagac gatgccggct tcttccatca atttgacccg gcgggcgacg
     1441 acccattttt cgagtttgaa gtcggggatg ccgtagcgga ggaggccgcc gggctggtca
     1501 tcgcgttcga agacggtgac ggagtggccg gctgcgttga gctggtccgc ggcggcgagg
     1561 ccggcggggc ctgaaccgac gacggctact ttttttcctg tgcggagggc cgggtggttt
     1621 gcttttacga agcctttgtc gaaggcgatc tcgatgatat ggcgttcgat ctcttcgatg
     1681 gcaacggggg gctggttgat accgagtacg cagctgcttt cgcagggggc ggggcagatg
     1741 cggccggtga actcggggaa attatttgtc gaggtgagga tctcgtaagc ttcctgccat
     1801 tgctggcgat agacggcgtc gttgaattcg gggatcacat ttcccagggg gcagccgcta
     1861 tgacagaagg ggatgccgca gttcatgcac ctggcggcct ggtggttgag ttgtgcgcca
     1921 tcgtaggtgt tgacgaattc tttatagtca tttttccgct cgtgcacggg gcgtttggag
     1981 gggagttgac gatcgaattc caaaaagcct gtgggtttgc ccatggtcgt atattgctgt
     2041 ttgtaattat ttcaatttgg aagagcgcac cggctacaga gaacaacgga gtggctgagt
     2101 cacacatgag agcctactcc gcgttctcaa aagtttctac ttgtgagtag ttctctgtgg
     2161 ccgggctgct ttttccaata atactttttt atagtcggtg gggaacacct tgacaaaatg
     2221 ttgtagctgg ttctcgaaat cgtcgaggac gaatttggcg acgttgctgc ccgtgtaggc
     2281 atagtgtcgt tggatcatgt ccctgagttc ggagatatcc tcgccggcga cgggatcgag
     2341 gtcgaccatt tcccggttga catttctacc gaaggttcct cgtacatcat agacataggc
     2401 gatgccgccg ctcatgcccg cggcgaagtt gcggcccgtg ttgccgagga tgacgatgcg
     2461 tccggcggtc atgtattcac aaccgtggtc gcctacgcct tcgacgacgg ccttgacgcc
     2521 cgagttgcgg acggcgaagc gttcgccggc cttgccgcgg ataaaggctt ccccggaggt
     2581 ggcgccgtag aaggcgacat tgccgatgat gctgttctct tcggggacga agccggcgtt
     2641 ctggcttggg tagacgatga gtcttgcgcc ggagaggcct ttaccgaagt agtcgtttgc
     2701 gtcgccttcg agttccagga ctacgccatg ggtgttgaag gcgccgaagc tctggccggc
     2761 gg
//
LOCUS       DATGFG010000362         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1327672, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000362 DATGFG010000000
VERSION     DATGFG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327672"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..673)
                     /locus_tag="VKQ52_06910"
     CDS             complement(155..673)
                     /locus_tag="VKQ52_06910"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ86948.1"
                     /translation="MTKAVHTEFWLRGPVPGIPPLLQPVAHALLQACQEVNDLMAAFP
                     DELLWERPAGVASPGFHLQHLAGVLDRLFTYARDHGLNEAQLADLATEGSPIEGQSTV
                     AQLLNRFNIEIDKALEQVGQTPEDSLTAHRPVGRARLPSTVGGLLFHAAEHIQRHTGQ
                     LLVTARILRQPL"
     gene            complement(709..1095)
                     /locus_tag="VKQ52_06915"
     CDS             complement(709..1095)
                     /locus_tag="VKQ52_06915"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ86949.1"
                     /translation="MQSFFFIRHEGRFVKVDFRHILYIEARKNYTRLVLEDRPTALVL
                     VTLKQWEKLLPRDLFCRVHRGYIVSIDKIISFDNKYVYLPGERISIGEQYRNALPDVV
                     TILANESSRETVRNTHKDVLSELELQ"
     gene            complement(1245..1805)
                     /locus_tag="VKQ52_06920"
     CDS             complement(1245..1805)
                     /locus_tag="VKQ52_06920"
                     /inference="COORDINATES: protein motif:HMM:NF020914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RteC domain-containing protein"
                     /protein_id="HLZ86950.1"
                     /translation="MSPEYQEYYQQLLTAFHSHEATARTEMEMIEACFKSSLDYWGKV
                     CKLVRANGFRDTRDEICFFREVKPAFASFIEYYTFRYHAILFAPVNDLIELKRFWRWE
                     ERKMQRFYEDNREFCRYMREGSTHRDNEYFLRTADRGAVSPTADLIHDLDTELVSAKD
                     PLLTIMKAYELYGQYINQKLVSYLEA"
     gene            2456..4015
                     /locus_tag="VKQ52_06925"
     CDS             2456..4015
                     /locus_tag="VKQ52_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86951.1"
                     /translation="MANRFSDTLFQLIHSLEKSEKRHFKLYIKRSSTKEDLKIVQLFD
                     ALDKLNDYDEKSLLKKLPGIEKPQLSNLKTHLNKQILASLRLLKSADSIDLQLNEMFD
                     YAHILYKKGLFQQSLRWLDRAKETARANQKFNFLVQVLALEKRIETLHITHSMQMRAE
                     QLSAESRAVIDRIDMVARLSNLALLVYSWYIQNGHARNEKDEERIRQYLKEQLPANAW
                     EQTGFYERLYLYQSYNWYAFIRQDFLMYYRYSQKWLDLFEEQPLMIRVETGHYIKALH
                     NLLNAHFDLRNYQKFEVVLRKFEAFSQTDRVRENDNFRIQSFVYITTAKINQVFMLGN
                     FTGGLTMVPEIEQKLAEYDLFIDRHRILVLNYKIASMYFGAGDYGTCIDYLQRIINDQ
                     IDLRNDLQCYARVLHLMAHYELKNFDLMESLTKSVYRFMAKRERLTRVEEEMFKFLRT
                     SFQTTRAGLRVEFEKFLEKIKDFERNRFEARAFAYLDLVSWVESKVSQRPMSEIIAEK
                     YRSSKRKMVAA"
     gene            complement(4025..>4493)
                     /locus_tag="VKQ52_06930"
     CDS             complement(4025..>4493)
                     /locus_tag="VKQ52_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86952.1"
                     /translation="IAASEAITAKGAYPERPFVLLTQQSLFDPSRAPDGKHTGWAYCH
                     VPGGSREDRTAVIEAQVERYAPGFRDRILARHTMDTAQLEEYNPNYIGGDINGGIMDI
                     RQLFTRPALRRSPYRTSAKGIYLCSSATPPGGGVHGMCGYHAARRALKDIFHL"
BASE COUNT         1089 a         1062 c         1256 g         1086 t
ORIGIN      
        1 aatccataat attgatcgta cagcattttc tggcattgcc tgtcctgctg cgcgcaaccc
       61 gcgattatat gaagtagttt ttccaaaagt agacttctta cttacctgta ttctaatggg
      121 ttccaggcat attgtagatt aaacgcctta tttttcacag gggttgcctc aggatccggg
      181 cggtgacaag taattgaccc gtatggcgtt ggatatgttc ggctgcatga aaaaggaggc
      241 cgccgacggt ggagggaagc cgggcgcggc cgacgggccg gtgggccgtc aggctgtctt
      301 ccggagtctg gccaacctgt tctaaagcct tgtcgatctc tatgttaaat ctattcagca
      361 attgtgcgac ggtgctctgg ccttcgatgg gggagccttc ggtggcgagg tctgccagtt
      421 gggcctcgtt gaggccgtgg tcgcgtgcat aggtgaaaag ccggtcgagg accccggcga
      481 ggtgttgcaa atggaagccc ggggaggcga cgccggcggg ccgttcccag agcagctcgt
      541 cggggaaggc agccatcagg tcattcactt cctggcaggc ctgcaggagt gcgtgtgcaa
      601 cgggttgcag caatggtggg atgccgggaa cgggtccgcg gagccagaac tccgtatgta
      661 ctgcttttgt cattgccgag gcttggatcg cccggcaaat taccgatttt attgcagctc
      721 cagctcagag aggacgtctt tatgcgtatt gcggaccgtc tcgcgggaag attcgttggc
      781 gaggatggtg accacatccg gtaaagcgtt gcgatactgt tcgccgatgg atatcctctc
      841 tcccgggaga tagacgtatt tgttgtcgaa cgagatgatc ttgtcgatcg acacgatata
      901 gccacggtgt acgcggcaga acaggtcgcg gggcaggagt ttttcccact gtttgagcgt
      961 gaccaatacc agggcggtgg gcctgtcttc caggaccagc cgggtgtagt tcttccgggc
     1021 ctcgatgtag aggatgtggc ggaaatccac tttcacgaac ctaccttcgt gacggataaa
     1081 gaaaaaggat tgcacttgct aaagtttact gtcaataaaa gggttattgc ggcgggtcgt
     1141 tgccgaaggg ataagaaggt gtctaaattc cgtcaccggc aaacagggat tccagcagca
     1201 gcacatagct gttgcgtttg tccgggaatc gttttgacgg gtaatcaggc ctcaagataa
     1261 ctcaccagtt tttgattaat gtattgtccg tataattcat atgccttcat gatcgtcagg
     1321 agtgggtctt ttgccgagac cagttcggta tcgaggtcgt ggataagatc ggcggtggga
     1381 ctgacggcgc cgcggtccgc tgtacgtaaa aagtactcgt tgtcccggtg ggtactgcct
     1441 tcgcgcatgt agcggcagaa ttcccggttg tcctcataga agcgttgcat cttccgttct
     1501 tcccagcgcc agaaccgctt cagttcaatg aggtcgttca ccggtgcaaa gaggatcgcg
     1561 tgataccgga aggtatagta ctctataaag gaggcgaaag cgggttttac ttccctgaaa
     1621 aagcaaatct cgtcacgtgt atccctgaaa ccattggccc tgaccagttt acatactttt
     1681 ccccagtaat ccagactgga tttgaaacag gcttcgatca tttccatttc agtacgagca
     1741 gtagcttcat gggaatggaa ggcagtcaac agttgttggt aatattcctg atattcaggc
     1801 gacatagttt ccttaagaaa ggtttaaaga gccatccggc gtccctgtat gtgttttcgg
     1861 caataaaaca gcagggcctt gtagacacaa accccggagg actcaaaggt cacatgagaa
     1921 aacactacac tttatataac taaaaacaaa cgaaatcttt caaaaaaaat agtaagtgtc
     1981 aacaggatgg taaatctata cgtttcccgg gcagcatttc aaaaaactac attaactctc
     2041 ctaatgacta caccaagcgt agtattttaa ccaccaaatg gaaaagtttt agcaaccttt
     2101 tagcaggcgg gggaaaggaa aataaaaagg ggggaaaaag ccgttatttg ttaattgtta
     2161 gctagattgc aaaaaaaagg cccgctgtag aaacagccga cctctaacga ttgcttgcca
     2221 tatgaaaaaa ggtagtaata tctttttcgc tgattaactc agcgttattt cgaatgagcc
     2281 tctaacgaat gcctgttgaa cgattgctgc catattttca tctcattctc tgcgtaaagg
     2341 tactcttcaa aggcttgccg ctcgaatcaa attatcggtg agttaatcat gtcaaaatga
     2401 taattttagt gttgaaatcc tttactggat tgagtttttg gataatccca tcattatggc
     2461 caaccggttt tcagacacct tattccaact tatacattcg ctcgaaaagt cagaaaaaag
     2521 acattttaaa ctgtatatca aaagaagctc gacaaaggag gatctcaaga tcgtccagtt
     2581 gttcgatgcg cttgataaat tgaatgatta cgacgagaag agcctcttaa agaagttgcc
     2641 ggggatcgaa aagccccagt tgtcgaatct caagacccat ctgaataaac aaatcctcgc
     2701 cagtctccgg ttgctgaaga gtgccgacag catcgatctg cagttgaacg aaatgttcga
     2761 ctatgcccat atcctttata agaagggttt gttccagcag agcctgcggt ggctggacag
     2821 ggcaaaggag acggccaggg ccaaccagaa attcaatttc ctggtgcagg tgctggcgtt
     2881 ggagaagcgg atcgagaccc ttcacattac ccatagcatg cagatgcggg cggagcagct
     2941 ttcggcggag tcccgggccg tgatcgaccg gatcgacatg gtggcgcggc tgtccaatct
     3001 cgccctcctg gtctatagct ggtatatcca gaacgggcat gcgcggaacg agaaggatga
     3061 ggagcggatc cggcagtatc tgaaggagca gttaccggca aatgcctggg agcagacggg
     3121 cttttacgag cggctttatc tttatcaaag ttataattgg tatgctttta tccgccagga
     3181 cttcctgatg tattaccgtt attcccagaa gtggctcgac ctgttcgagg agcaacccct
     3241 gatgatacgg gtcgagacgg ggcactatat caaggcattg cacaacctgt tgaatgcgca
     3301 tttcgatctc cgtaattacc agaagttcga ggtcgttcta cggaagttcg aggctttttc
     3361 ccagaccgac agggtccggg agaacgacaa tttccgcatc cagagctttg tctatatcac
     3421 cacggccaag atcaaccagg tctttatgct gggaaatttc accggggggt tgacgatggt
     3481 gcccgaaata gagcagaagc tggcggaata tgatcttttc atcgatcgtc accgcattct
     3541 cgtgctgaac tataagatcg cttcaatgta ttttggcgcc ggtgactatg gaacctgtat
     3601 cgactacctg cagcggatca tcaacgacca gatcgacctc cgtaacgatc tgcaatgtta
     3661 tgcccgggtg ctccacctga tggcgcatta tgaactcaag aacttcgatc tgatggagtc
     3721 gctgactaaa tcggtatacc ggttcatggc caagcgggag aggctgacac gggtggagga
     3781 ggagatgttc aaattcctgc gcacttcttt ccagaccacg cgggccgggt tgcgggtgga
     3841 gttcgagaag ttcctggaga agatcaagga tttcgagcgg aaccgcttcg aggccagggc
     3901 ctttgcctat ctcgacctgg tctcctgggt ggagagcaag gtctctcagc ggccgatgag
     3961 cgagatcatc gccgagaagt atcgcagcag caagagaaag atggtggcgg cctaggacgc
     4021 ggggctagag atggaagata tccttcagcg cccggcgggc ggcgtgatat ccacacattc
     4081 catggactcc cccacccggc ggcgtcgccg atgagcagag atagatccct tttgccgatg
     4141 tccggtaggg cgagcggcgg agggccggcc gggtgaagag ttgccggatg tccattattc
     4201 ccccgttgat atccccacca atgtagttag ggttatattc ttccaattgg gccgtgtcca
     4261 tggtatggcg ggccaggatg cgatcgcgga agcccggggc ataacgttcc acctgcgctt
     4321 cgatgacggc agtcctgtct tcccgggagc cgccgggtac atggcagtag gcccagcccg
     4381 tgtgcttgcc atcgggggca cgggagggat cgaagaggga ttgctgggtc agcaggacga
     4441 aggggcgttc ggggtaagcg cctttcgccg tgatcgcttc cgaggccgcg atc
//
LOCUS       DATGFG010000363         4468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_26734, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000363 DATGFG010000000
VERSION     DATGFG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26734"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VKQ52_06935"
     CDS             complement(<1..958)
                     /locus_tag="VKQ52_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLZ86953.1"
                     /translation="MKRRSFLIGVVFPFFLISCHKDLPDAVNAQDYPGDSYPELFEAF
                     WTGMNTNYIFWSIDTTNWDAVYTRYKPLFAQLTSFNSGNEAKAEQYFTEMTSGLIDGH
                     YNLTFEATGRSFSPSLSRKLTADANFLQDSAYPATSLNTLIGLVYIDSGSLRMGSDPV
                     TLGTSHTSLTAISGTIGGSILYLYFSNFSISQAATYTTPVFNYFFSTLRSLSGIKGVI
                     LDLRGNPGGEVSDLDYIVGQMVNEPYTFGYTRTKGGANRLDYTPWAPAIVKPWNGGTK
                     VTAPIVVLADHLSTSMAEITTMAVKTLPNGKFIGTRTWGATGP"
     gene            complement(955..1668)
                     /locus_tag="VKQ52_06940"
     CDS             complement(955..1668)
                     /locus_tag="VKQ52_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ86954.1"
                     /translation="MKKCAVFILLLYSIRGNGQWYAGVEAGGTRDYLITTTGGEAFTS
                     YHGATGFLVDIPVQYRVNDWLAFESGAGFEQKNYMMSRTGFFQGVGQKNSNGYLQLPL
                     LGDCSFGGKKIRGFVKAGVYGAYWLTGRIKGNSPNILNTTDTTTLNPSGVLNVDNGYT
                     YNQRYAFSSKDNRIELGWLAGAGIRYQVNGLLCLYLEGRYAGAFTDQQKKYQLNQTPR
                     YNSTYGIGIGSLFNLKRIL"
     gene            1827..2222
                     /locus_tag="VKQ52_06945"
     CDS             1827..2222
                     /locus_tag="VKQ52_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86955.1"
                     /translation="MPITITVKDETTGGRIFNETPITFENELTTVKAIIVARVTAEVE
                     AYNTGLPEYYKGLVQPSDAENTLNGYRLKARRQIDPEQQCAVALDAFKKNGYFVLIDN
                     IQAESLDQMVVINSDTNISFVKLTPLIGG"
     gene            2240..>4468
                     /locus_tag="VKQ52_06950"
     CDS             2240..>4468
                     /locus_tag="VKQ52_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HLZ86956.1"
                     /translation="MADQQADKLLDEMIAEARTTYFHAYSPDQAGGAIVSRYLPADEA
                     ARKRILVYFGHKLVEFYKADKQLRYEKRIREQVILFVLRAALDELHTWNDEEIHSLIS
                     NWHSVMEEDYFWFSYPVEKIADQLLKKIGVSGLTEPLKESLKLARYPKHLNATKDRRK
                     LNDRIDLVIQGQQELPVNRLDPLGGRIADFIGSIDDPEEKDGWTKLVECCYSIGDKSV
                     PTKKWLGDAGQIMGRLDKTVFIGKMQEWLATAKATLAEAHRSKGYQRDVFSENIQELL
                     KALIWCTAFANSQELLNDLDEYAALAYRKKPGVGPLSAKTGNACMYAFSLLPVKEGIS
                     KLVKFRTKIKNNTILKGIDRIIREVSEKNNVSWDELEELSVPDLGLDSQGELRVPIDG
                     FVAVYSVKGTADAQLVWEKDGKAEKAVPASIKEGFGAQLKNIKHSIREIESLLPVYKE
                     KIEQSFLRQRTWGYGQWAKHYLHHPLMGILAKKLIWYFSDAGTKTQGIWLNGEIVDVN
                     EKVLHFDEGTEVQLWHPIGFGEDEIAAWRNFLQKHRITQPFKQAYREIYVVTDAELAT
                     DTYSNRFAAHILRQHQFAALCKQRGWKYRLMGAFDSHNTPYIALDNWNIRVEYLVDAD
                     WQGATSGAGIFSYISTDQVRFYRDNQRLQMMEVPALVFTETMRDIDLFVGVASIGNDP
                     SWQDGGNATADTYWRDYSFGDLSESAKIRGEILRGLIPRLKIAAKCSFDGRYLRVQGR
                     IRS"
BASE COUNT         1152 a         1110 c         1231 g          975 t
ORIGIN      
        1 gcggaccggt ggcaccccag gtccgggtgc cgatgaattt accgttcggc agggtcttca
       61 ccgccatcgt ggtgatctcc gccatactgg tagagagatg atcggccagt acaacgatcg
      121 gcgctgtcac ctttgtcccg ccattccaag gttttacgat ggccggcgcc cagggggtat
      181 agtcgagccg gttggctccg ccctttgttc gcgtataacc gaaggtataa ggctcgttca
      241 ccatctggcc gacgatataa tccagatcgc tcacttcgcc ccccggattt ccccgcagat
      301 ccaggatgac tcctttgata ccggacaggg accggagcgt ggaaaaaaag taattgaaca
      361 cgggggtggt atatgtcgcc gcctgggaga tagaaaaatt actgaaataa agataaagga
      421 tggaaccgcc aatggtaccc gaaatagcgg taagcgaagt gtggctggtg ccgagcgtca
      481 ccgggtccga acccattcgg agactgccgg agtcgatata aaccaaaccg atcagcgtgt
      541 tcagggacgt ggcgggataa gcgctgtcct gtagaaaatt cgcatccgcg gtgagcttcc
      601 tgctcaggga cggactaaag gaccgacccg ttgcctcgaa agtaaggttg taatgaccgt
      661 cgatcagccc gctcgtcatc tcggtgaagt attgctccgc ctttgcttcg ttgccgctgt
      721 taaaactggt gagctgcgca aaaagcggct tgtaccgggt atagaccgca tcccagttgg
      781 tggtgtcgat actccaaaag atataattcg tattcatgcc ggtccaaaac gcctcgaaaa
      841 gctccggata gctgtcgccc ggataatcct gtgcgttgac ggcatccggc agatccttat
      901 ggcaggaaat aaggaagaag ggaaaaacaa cacctatcag gaaggatcgc ctcttcataa
      961 gatccgcttt aggttgaaca aactgccgat accaatgcca taggtggaat tataccgggg
     1021 ggtctggttc agctgatact tcttctgctg atcggtaaaa gcgcccgcat atcttccttc
     1081 gagatacaaa cacaaaagcc cgttcacctg gtaacggatg ccggcgccgg ccagccagcc
     1141 gagttctatc cggttatcct tgctgctgaa ggcataccgt tgattatacg tatacccatt
     1201 gtccacgttg agcactccgg agggattaag ggtagtcgta tcggtggtgt tcagaatatt
     1261 cggactgttg ccctttatcc ggcccgtcag ccaataggcg ccatataccc ccgcttttac
     1321 aaacccgcgt atttttttcc cgccaaaact gcaatctccc agcagcggca actgcaggta
     1381 cccattactg ttcttttgcc ctactccctg gaagaaccct gtcctggaca tcatgtagtt
     1441 cttttgttcg aacccggcgc cggattcgaa tgcgagccag tcattcaccc gatactgtac
     1501 ggggatatcg accaggaaac cggtggctcc atgataactg gtaaaagcct ctccgccggt
     1561 ggtggtgatg agatagtccc tcgtgccacc cgcctcgacc cctgcatacc attgcccatt
     1621 tccgcggatg gaataaagca acaatatgaa tactgcacac tttttcatga atcgtacacg
     1681 gttaaacttt tcctttatga aacgaatgta caccttcctg ccgggagcag cagtcctttg
     1741 tttttgatgc cgcggagggg ttccgggaaa aaattagccg ttggataacc taaaataggt
     1801 aagtttactt tacaactact gacgatatgc ctataacgat cacggttaaa gatgagacca
     1861 ccggcggccg gatcttcaac gagaccccta tcaccttcga gaacgaattg acgacggtaa
     1921 aagcgattat cgtggcccgg gtaacggccg aggtagaggc ctacaacacc ggactgccgg
     1981 aatactataa agggttggtg caaccttccg atgcggaaaa caccctgaat ggataccggc
     2041 taaaggccag gcggcagatc gatccggaac agcagtgcgc cgttgcgctg gatgccttca
     2101 aaaagaatgg ctattttgtc ctgatcgata atatccaggc ggaatcgctg gatcagatgg
     2161 tggtgataaa tagcgatacg aatatcagct ttgtaaaact aacgccattg atcggcgggt
     2221 aaatgtaaat tactgataca tggctgatca acaggctgat aaactgctgg acgagatgat
     2281 agcggaagca agaaccacgt acttccatgc gtattcaccc gaccaggccg gcggggcaat
     2341 agtgtccagg tacctgcctg ccgatgaagc cgcaagaaaa aggatactgg tttactttgg
     2401 gcacaagctc gtggagtttt acaaggccga taaacaactt cggtatgaga aaaggatccg
     2461 cgaacaagtc atcctctttg ttttgcgtgc cgctcttgac gagcttcata cctggaacga
     2521 tgaagagatc cactccctga tcagcaactg gcattcggtg atggaagagg attatttctg
     2581 gttctcttat cctgtcgaaa aaatagccga tcaactattg aagaagatcg gggtttccgg
     2641 gctgacggaa ccattgaagg aatctttaaa actggcaaga tacccgaaac atctgaacgc
     2701 aacgaaagac cggagaaagc tgaatgaccg gatcgatctg gtcattcagg ggcagcagga
     2761 gctgcccgtc aaccgcctgg acccccttgg cggccggata gccgatttta tcgggagcat
     2821 cgacgatccg gaagaaaagg acggatggac aaagctggtt gaatgctgct attcgattgg
     2881 ggacaagtcg gttcccacca aaaaatggtt gggagacgcc gggcagataa tgggccgtct
     2941 agacaagacg gtgttcatcg gaaaaatgca ggaatggctg gcgacggcaa aagccaccct
     3001 tgccgaggca caccgatcga aagggtatca acgggacgtc tttagcgaga atatccagga
     3061 attgctaaag gccctcatct ggtgtaccgc ctttgccaac agccaggaac tgctcaacga
     3121 cctggacgaa tatgctgccc tggcgtatag gaaaaagccc ggtgtgggtc ccttgtcggc
     3181 aaaaacgggg aatgcctgca tgtatgcttt ttccttgttg cccgtgaagg aagggatctc
     3241 gaagctggta aaattccgga cgaagatcaa gaataatacc atcctgaaag gcatcgacag
     3301 gatcattcgg gaggtctcgg aaaaaaataa tgtatcgtgg gatgagttgg aggagttgag
     3361 cgtgcctgat cttgggctgg atagccaggg tgagctgagg gtacccatcg acgggttcgt
     3421 ggccgtctac tccgtaaagg ggactgccga tgcgcagttg gtctgggaaa aagacgggaa
     3481 ggcggagaag gcagtccctg catccataaa ggaaggtttt ggcgcgcaac tgaaaaatat
     3541 aaagcattcg atcagggaga tcgaatcgct gttacctgtt tacaaggaga agatcgaaca
     3601 atccttcctc aggcaaagaa cctggggtta cggacaatgg gcaaagcact acctccatca
     3661 tccgctgatg ggaattctgg caaagaaact catatggtat tttagcgatg ccggcacaaa
     3721 aacacagggc atctggctaa acggggagat cgtggacgtc aatgagaagg tgctgcattt
     3781 cgacgaaggg acagaggtcc agctctggca ccccatcggg ttcggggaag acgagatcgc
     3841 ggcatggcgg aatttcctgc aaaaacaccg gatcacccag ccattcaaac aggcctatcg
     3901 ggagatctat gtagttaccg atgcggaatt ggcgacggat acctactcca accgctttgc
     3961 cgcgcatatc ctgcgccagc accagtttgc cgccctgtgt aaacagcggg gatggaaata
     4021 ccggctgatg ggtgcattcg attcccacaa cactccatac atcgcattgg acaattggaa
     4081 tatccgggtg gaatatcttg tcgatgcgga ttggcagggc gcgaccagcg gggcgggcat
     4141 tttctcttat atctcgactg accaggttcg tttttaccgg gacaaccaac gcctgcaaat
     4201 gatggaggtc cccgcgctcg tttttacgga gaccatgcgg gatatcgacc tgttcgtagg
     4261 cgtggccagc atcgggaacg acccgtcctg gcaggacggc ggaaatgcga cggccgatac
     4321 gtactggcgt gattattcat ttggtgatct gtcggaatcg gcgaaaataa ggggcgaaat
     4381 actgagggga ttgatcccga gactaaagat tgccgccaaa tgctccttcg acggccgata
     4441 cctgcgggtt cagggcagga tcaggagc
//
LOCUS       DATGFG010000364         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_618064, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000364 DATGFG010000000
VERSION     DATGFG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618064"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..599
                     /locus_tag="VKQ52_06955"
     CDS             198..599
                     /locus_tag="VKQ52_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86957.1"
                     /translation="MKNSQWYGMVAALLVVAACFMPWAYFPDLQKEFTGFFSEQNRYG
                     RPGKVLVALSVVEMVFFLIPKVWAKRANIFVAAVGFAWGIKSYILYSACYRGTCPEHR
                     IGIFLVLAGVLLVLAASLLPDLPVKEDQDRG"
     gene            complement(635..1357)
                     /locus_tag="VKQ52_06960"
     CDS             complement(635..1357)
                     /locus_tag="VKQ52_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HLZ86958.1"
                     /translation="MHDIDTSISAGHAEQHMRSSEILTDIVIGMSDGLTVPFALAAGL
                     SGAVGQAHLNLIWIAGIAEISAGSIAMGLGGYLAGKTEVDHYNSELKREYEEVERLPE
                     REKEEVREFFSGLGLSRDVQDKAVDEMTRDKDKWVDFMMKYELGLDKPDPRRARKSAF
                     NIGISYVVGGLIPLSPYFFVSSGITGLRISATITLCCLFIFGYFKSKLTGVNPLSGAI
                     RVMIIGALAAGCAFAVARMIQS"
     gene            complement(1417..2658)
                     /gene="rocD"
                     /locus_tag="VKQ52_06965"
     CDS             complement(1417..2658)
                     /gene="rocD"
                     /locus_tag="VKQ52_06965"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HLZ86959.1"
                     /translation="MHATTTLTARSLHYLELEEQYSAHNYHPLPVVLDRGAGVHVWDV
                     DGRRYYDCLSGYSAVNQGHCHPRIVAALQEQAARLTLTSRAFHSDLLGEYARYITSYF
                     SYDKVLPMNTGVEAVETAIKLCRRWGYQVKDIPAGKARIIVCANNFHGRTTTVISFST
                     DPDSNRDFGPYTPGFVIIPYNDTPSLARALEDPHVAGFLVEPIQGEAGVVVPDEGYLR
                     QAKQLCEAAGVLFIADEIQTGLSRTGRLLACDHEAVRPDILILGKALSGGTLPVSAVL
                     ADDAIMLTIRPGEHGSTYGGNPLACKVAIAALQVLKDEDMAGNAAAMGELFRKEIRAL
                     QSPHINAVRGKGLLNAIVIDHPRPDAAWELCLALKDNGLLAKPTHGDKIRLAPPLLIT
                     REQLLECVTIVKNSLAVLENH"
     gene            complement(2780..>3407)
                     /locus_tag="VKQ52_06970"
     CDS             complement(2780..>3407)
                     /locus_tag="VKQ52_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86960.1"
                     /translation="TSNLCEKAYRLLKADFPTLPTVHIHLYKNIPIGAGLGGGSADGA
                     FTLLALNKKFQLNLDTQRLLHYAAQLGSDCPFFILNTPCLGGGRGEQLTPIPLDLGAY
                     SFVLVNPGIHIGTAQAFSGCTPREDNISLPDILQQPVATWPQHLTNDFEGPVFQLHPE
                     LREIKTTLYACGALYASLTGSGSSFYGIFEKHNTPSSLPLSWNYRILP"
BASE COUNT          638 a          747 c         1276 g          746 t
ORIGIN      
        1 gtagtctttg tccgtcagga aggaggtatg gtagaaacat tgggcgaaga ggcccgggga
       61 ggaaaggaag gaggtcaaca ggagaaggct ggcaataggg gatttcattc ctgtacagga
      121 ccatctggcg cgccggatgg ctgcgagcgc aggtgttaat gatcttggaa aatggggatg
      181 tggtgtatat tcgcggcatg aaaaattctc aatggtacgg aatggtggcg gcgttgctgg
      241 tggttgcggc ctgttttatg ccctgggcgt attttcctga cctgcagaag gagttcaccg
      301 gttttttttc cgagcagaac cggtatgggc ggccggggaa ggtgctggtt gcgcttagtg
      361 tggtggagat ggtctttttc ctgatcccga aggtctgggc gaagcgcgcc aatattttcg
      421 tggcggcggt gggttttgcc tgggggatca agtcgtatat tttgtattcg gcctgttacc
      481 ggggcacctg tcccgagcac cggatcggca tatttttagt cctggccggg gtgttgcttg
      541 tgctggcggc gagtctgcta cccgatctgc cggtgaagga ggatcaggac aggggatagg
      601 ggcgatgccc gggatttgtt gccattttgt cggatcagga ctgaatcatg cgtgctaccg
      661 cgaaggcaca gccggccgcg agtgcgccga tgatcattac gcggatggcg cccgagaggg
      721 ggttgacacc ggtgagtttg cttttgaaat agccaaagat gaacaaacag cagagggtga
      781 tggtggccga gatgcggagg ccggtgatgc cactgctgac gaagaaatag gggctcaggg
      841 ggatgaggcc gccgacgaca taggagatgc cgatattgaa ggcgcttttg cgggcgcggc
      901 gcggatcggg tttgtcgagg ccaagttcgt atttcatcat gaagtcgacc catttgtcct
      961 tgtcgcgggt catttcatcc actgctttat cctggacgtc gcggctgagg ccgaggccgg
     1021 agaagaattc gcgcacctct tctttttccc gttcggggag tctttcgacc tcctcgtatt
     1081 ccctttttag ttcggaatta tagtgatcga cctcggtctt gccggcgagg tagccgccca
     1141 ggcccatggc gatggagcct gccgagatct cggcgatacc ggcgatccag atgagattga
     1201 gatgggcctg accgacggcg ccgctaagac cagccgcgag ggcgaagggg acggtgaggc
     1261 catccgacat cccgatgacg atgtcggtga gaatctctga actgcgcatg tgttgctcgg
     1321 cgtggccggc ggagatagac gtgtcgatat cgtgcatgcg gtaaatttag cgattttcgg
     1381 gaacgacgtg ttgagtgatt ttcgggaacg gcgggtttag tgattttcaa gaacggcgag
     1441 gctgttcttt acgatggtca cgcattcgag cagctgttcg cgggtgatca ggaggggcgg
     1501 agcgaggcgg atcttgtcgc catgggtcgg ttttgccagc agcccattgt ctttcaatgc
     1561 gaggcagagc tcccaggccg catcggggcg cgggtgatcg atgacgatgg cattcaggag
     1621 gcctttgccg cggacggcgt tgatatgggg cgattgcagg gcccggatct ctttgcggaa
     1681 cagttcgccc atggctgcgg cgttgccggc catgtcttcg tcttttagta cctggagggc
     1741 ggcgatggct accttgcagg ccagcgggtt gccgccatag gtggaaccat gttccccggg
     1801 gcggatggtg agcatgatgg cgtcgtcggc caggacggcg gatacgggca gcgtgccgcc
     1861 gctgagggcc ttgccgagga tgaggatatc ggggcggact gcttcgtggt cgcaggccag
     1921 caggcggccg gtgcggctga ggcccgtttg gatctcgtcc gcgatgaaca ggacacccgc
     1981 ggcttcgcag agctgttttg cctggcggag atagccttca tcggggacga ccacgccggc
     2041 ttcgccctgg atgggttcga cgaggaagcc ggcgacatgc gggtcttcga gggcgcgggc
     2101 gagggagggg gtatcgttat aggggatgat gacgaagccg ggtgtatagg gaccgaagtc
     2161 gcggttggaa tcggggtcgg tgctgaagga gatgacggta gtggtgcggc cgtggaagtt
     2221 attggcgcaa acgatgatcc tggctttgcc ggccgggatg tcttttacct ggtagcccca
     2281 gcggcggcaa agctttatcg ccgtctccac ggcttcgacg cccgtgttca tcggcagtac
     2341 tttgtcatag ctgaaatagc tggtgatata gcgggcgtat tcgccgagga ggtcgctgtg
     2401 gaaggcgcgg gaggtcaggg tgaggcgggc ggcctgttcc tggagggcag cgacgatgcg
     2461 ggggtggcaa tggccctggt tgacggccga gtagccgctg aggcagtcgt aatagcgacg
     2521 gccatccaca tcccagacat ggacgccggc gccgcggtcg agcacgacgg ggagagggtg
     2581 gtagttgtgg gcgctgtatt gttcttccag ttccaggtaa tggagggacc gggccgtgag
     2641 ggtggtcgtt gcatgcataa aaagaggatg taaaagtggt agaaagatga aaatcagaca
     2701 attagtactg ggcgatatcg tcagcagtgg tgatcgagga aatctaaatt ttggcccagg
     2761 aataacatgt cggtgagatt tacggcaaga tacgataatt ccaggaaagg ggcaaggagc
     2821 tgggggtgtt gtgtttttcg aagatgccgt agaaggagga gccgctgccg gtgagggagg
     2881 cgtagagggc accgcaggca tagagggtgg tctttatttc gcggagctcg gggtggagtt
     2941 ggaagacagg gccttcgaag tcattggtga ggtgttgggg ccaggtggcg acgggttgtt
     3001 gcaggatgtc ggggagggat atattgtctt cccggggtgt gcagccgctg aaggcctggg
     3061 cggtgccgat atggataccg gggttgacga ggacgaagct gtaggctccc aggtcgaggg
     3121 gaatgggggt aagttgttcg ccacggccgc caccgaggca gggggtattg aggatgaaga
     3181 aggggcaatc gctgcccagt tgggcggcgt agtggaggag tcgttgggta tcgaggttga
     3241 gctggaattt tttgttgagg gccaggaggg tgaaggcgcc gtcggcggag ccgccgccga
     3301 ggcctgcgcc gatggggata ttcttataga gatggatatg gacggtgggg agggtgggga
     3361 agtcggcttt gaggagccgg taggctttct cgcagaggtt gctggtg
//
LOCUS       DATGFG010000365         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_495024, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000365 DATGFG010000000
VERSION     DATGFG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495024"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2848)
                     /locus_tag="VKQ52_06975"
     CDS             complement(<1..>2848)
                     /locus_tag="VKQ52_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLZ86961.1"
                     /translation="LGYLHQEALSRQKFVECPFLPGERMYRTGDLVRWRPDGQLEYIG
                     RLDEQVKIRGYRVEPGEIECVLEQSGLVSQAAVMAREEVNGGKRLVGYVVGKERYDRA
                     IVLEWLRSRLPEYMIPSLLVELETMPLMPSGKIDRGALPEVAVTLQTSAAPRTALERA
                     LVVIWEKLLDVEGIGIDDNFFELGGHSLLGIRLISAVRKELAIELPIGELFDHPTIRG
                     LSACIGSLSGLPVSGGVPVIVTGERPQRIPLSFSQERLWFIDQLEGSRQYYVSSVLRL
                     RGALRVEALSHALQEVINRHEVLRTVIIAEDGYPCQRVLGKDSWQLGIIPGEGYAWNT
                     AGLQEKVEQLTGALFDLSADHPLRADLIRLGEEEHMLVVTLHHIASDGWSTSILVNEL
                     VELYAAYAEGRASGLEPLGIQYADYAIWQRKHLEGDLLREKLAYWKGQLEGVEPLNLP
                     TDYVRPAVQSNRGSVLRFAIGKELTEGLRRLSLTEGTTLYMVLLAAFKVLLHRYSGQE
                     DICVGSAVANRTQRETEVLIGFFINTLALRSRVSDEVTFRQLLGQVRTMTLGAYEHQD
                     VPFEKVVEAVVKERNRSRAPLVQVVIGLQNIPATPEMRLAEVSWKQENVEHITTKFEL
                     SFIIEEDHAGLTVNAEYCRDLYTAESIGRMGEHYVELLRAVVADADSRVGELNMLQAA
                     ERRQLLAEFNDTRTDYPQDKTVVDLFEEQAKQTPAAVAVVYEDRSLTYRELDEQSNRL
                     GHYLRKRGVGAETLVPICVERSVEMIVGILGILKAGGAYVPVDADYPAERIGYMLSDT
                     GAKLVVSSRACRGLLGEGVEVIELDEEGDLPGPSHAVGGVAHPDQLAYVIYTSGSTGR
                     PKGVMIGHRNIVSLVRGVRYVSLTPGSILLSTGSPSFDATTFEYWGMLLNGGQLILCK
                     ESRLLDNELLKQEIVIRKINTIWFTASWFNQLADA"
BASE COUNT          612 a         1024 c          614 g          598 t
ORIGIN      
        1 gcatctgcca gttgattgaa ccagcttgcc gtaaaccaga tggtatttat ttttcttatg
       61 acaatctctt gctttagtaa ttcattgtcc agcagcctcg actctttaca aaggatcaac
      121 tgtcccccgt tgagtaacat accccaatat tcaaaggtgg ttgcatcaaa agaaggcgac
      181 ccggtagaca ataaaattga tccaggagtt aaactgacat aacgaacccc tcttaccagg
      241 ctaacaatat tccggtgccc gatcattact ccctttggcc tgcccgtcga ccccgaggtg
      301 tagatcacat acgccagctg gtcaggatgc gcgacgccac ctacggcatg cgacggcccg
      361 ggcagatcac cctcctcatc cagttctatt acctcaacgc cctcacccag cagaccccgg
      421 caggcacggc tgctcactac cagcttcgcc cccgtgtccg acaacatata cccgatgcgc
      481 tccgcgggat aatccgcatc caccggcaca tatgcgccgc cggccttcag tatccccagg
      541 atccctacga tcatctccac gcttctctcc acacagatgg gcaccagcgt ttccgcgccc
      601 acgccccgct ttctcaggta atgacccagc cggttcgact gctcgtccag ctcccgatag
      661 gtgagcgaac gatcctcata cactaccgcc accgccgccg gtgtctgctt tgcctgctct
      721 tcgaacaggt cgaccaccgt cttgtcctgc ggatagtccg tccgcgtatc attgaactcc
      781 gccagcagct gacgacgctc ggcggcctgc agcatattca gctccccaac ccggctgtcc
      841 gcatccgcca ccactgcccg cagcagctcc acataatgtt cccccatccg cccaatgctc
      901 tccgctgtat aaagatccct gcagtattct gcatttaccg tcaaccccgc atgatcctct
      961 tctattataa aggatagctc gaatttcgtc gtgatatgtt cgacattttc ctgtttccag
     1021 ctgacctctg ccaaccgcat ttcaggagta gcaggaatat tttgtaaccc gatgaccacc
     1081 tggactaaag gggccctgct ccggttgcgc tccttcacca ccgcctctac caccttctca
     1141 aagggcacat cctgatgctc ataggccccc agcgtcattg tcctaacctg acccagcagc
     1201 tgccggaagg ttacctcatc gctcacccgg cttcgcaatg ccagcgtatt gataaaaaaa
     1261 ccgatcaaca cctcggtctc acgctgcgtc ctgttggcta ccgcactacc cacacagata
     1321 tcttcctgcc cgctgtagcg gtgcagcagc accttgaacg ccgccagcag gaccatgtac
     1381 aacgtcgtcc cttccgtcag cgacaaccgc cgcagccctt ccgttagttc tttgccgatg
     1441 gcaaaacgca aaaccgaccc gcggttgctc tgtacggcag gacgcacata atccgtcggc
     1501 aggttcaacg gctccactcc ctccagctgc cccttccagt aggccagctt ctcccgcaac
     1561 aggtctcctt ccaggtgctt ccgctgccag atcgcatagt ccgcatattg tatccccaat
     1621 ggctccaatc ccgatgcgcg gccttccgca taagccgcgt acagctccac cagctcattc
     1681 accaggatag atgtcgacca gccatcagaa gcaatatgat gcagcgtaac caccagcata
     1741 tgctcctctt ctcccagccg gatcagatcc gctcgcagcg gatgatccgc cgacagatcg
     1801 aaaagagcac cggttaattg ttccactttc tcctgtaacc ccgccgtatt ccaagcatac
     1861 ccttctccgg ggatgatccc taattgccag ctatctttcc ccagcacccg ctgacaaggg
     1921 taaccatcct ccgctatgat gaccgtccgc aacacctcat gacggttgat cacctcctgc
     1981 aacgcatgcg acaacgcctc cacccgcaac gcaccccgca ggcgtaacac cgacgacaca
     2041 tagtactgcc ggctgccctc cagctgatcg atgaaccaca atcgctcctg gctgaaggac
     2101 agcgggatcc gttggggtcg ctcacccgtc acgatcaccg gcaccccgcc gcttaccggc
     2161 aagccggaaa gcgacccaat acaggccgac agcccccgga tcgtcggatg atcgaacagc
     2221 tccccgatcg gcagctctat agccagctcc ttgcgaacgg cggagatcag tcggatgccc
     2281 agcaacgagt gcccgcccag ctcaaagaaa ttatcgtcta tccctatccc ttcgacgtcc
     2341 agcagtttct cccatatcac caccaatgcg cgctctaacg cagtccgcgg cgccgcggat
     2401 gtctgcaagg ttaccgctac ttccggcaac gccccccggt cgatctttcc actcggcatc
     2461 aaaggcatcg tctccagctc taccaataaa gaagggatca tgtattccgg cagccgggac
     2521 cgcagccatt ccagcactat agcccgatcg tacctctcct ttcctacaac atagcccacc
     2581 agccgcttgc cgccattcac ttcttcacgc gccatcacgg cagcctggct caccaatccg
     2641 ctctgctcca gtacgcattc gatctctccc ggctccaccc ggtagccccg gatcttcacc
     2701 tgctcgtcca gccggccaat atactcgagc tgtccgtcgg gtcgccagcg tacaagatct
     2761 ccggtgcggt acatccgctc gcccggaaga aaggggcatt cgacaaattt ttgccggctc
     2821 agcgcctcct ggtggaggta tcccaatc
//
LOCUS       DATGFG010000366         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_595422, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000366 DATGFG010000000
VERSION     DATGFG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595422"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VKQ52_06980"
     CDS             <1..2083
                     /locus_tag="VKQ52_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86962.1"
                     /translation="DPAAKKALDSMGIKMPTAGGMSKQYDFATQHTSQQQLNAALGVE
                     TIPARDAARIASLPKGILSDAQLKVFVQGVRSAVGAHIDASARQYADQLAQNPDVRNN
                     GAAGLASVANGLWMIGNITPALELMGRAVAANPADPDNLNSYAVFLSALGGEQLALPI
                     LQKLNRDYPGNSTVLNNIGQAWFGLGDLTTSKKYLDSAIHFFGMHSQANYTEGVIEQS
                     KGNKDAAIDHLTKSLQNGYAPAKASRLSKLKGGLKDGDIGWNLPRPADALGLEKLVTQ
                     RPPFYTSADASTTLYPQWLAFRQSCIQKSATLQAEASQRALAAQKKMMKQISTGSLGS
                     GGTAQGGNIFFAQKAREMLHAVSSDESEFFLRMEDKEKKQLADLRASFLETSKNIRDI
                     NEKYTHKRQEQDSADGNKFDQFEGDDSHATKLLRQFNEQACKDGKKLVDDYYNHYNRI
                     LEDLAHEWISRETYFTNEIVYYSKYASLDDNEYEIKKAGAVAHFINLLGISGNASPVD
                     FYSDASGYMAGNLLCKEPPEKKPGGGKLQDYDDIHCDSHISLSIPGVVKSKWDCNTET
                     DEWSIGPSSGKYKENLNTGQFNVHGELGASTKISSLSAGPLKVGASAGAGVFVEATNT
                     GITDVGVTAHVDVKLGGSVDIPLGPNSDGKEDSHSVGKSVNLAGAEVRVGWNSGPSLT
                     GKGALSGISIK"
BASE COUNT          599 a          663 c          651 g          442 t
ORIGIN      
        1 agaccctgca gccaaaaaag ccctggacag catgggcatt aaaatgccga ccgccggagg
       61 aatgagcaaa cagtatgatt tcgcaaccca acacacctcg cagcagcagc tgaacgcggc
      121 cctgggtgtc gagacgatcc cggcccgcga cgccgcgcgg atcgcgagtc tgccgaaagg
      181 gatcctctcc gatgcacagc tgaaggtttt tgtgcagggg gttcgctccg ctgtcggcgc
      241 ccacatcgac gcttcggcca gacagtatgc ggatcagctg gcgcagaatc cggatgtacg
      301 gaacaacggc gccgcggggc tggcgagtgt ggccaacgga ctttggatga tcgggaatat
      361 caccccggcg ctggaactga tgggccgcgc agtagcggcc aatcccgcgg acccggacaa
      421 cctcaacagc tacgccgtat tcctgtcggc cctgggcggc gaacagcttg ccctgcccat
      481 cctgcagaaa ctgaaccggg actatcccgg caatagtacc gtactgaaca atatcggcca
      541 ggcctggttt ggactggggg atctgaccac atcaaagaaa tacctcgaca gcgcgattca
      601 tttcttcggc atgcactcgc aggccaatta caccgaaggg gtcatcgagc aatcgaaagg
      661 aaacaaggac gcggccatcg accatttgac aaaatcgttg caaaatgggt atgcgccggc
      721 taaagcaagc cggttgagca aactgaaagg cgggttgaaa gatggcgata tcggctggaa
      781 cctgcccaga cccgccgatg cgctgggact ggagaaactg gtcacccagc ggccgccttt
      841 ctacacctct gcggatgcca gcacgactct ttatccgcaa tggttggcct tccgccaatc
      901 ctgtatccag aagagcgcca cactccaggc ggaagcgtcg caacgggcgc ttgccgcaca
      961 gaaaaaaatg atgaagcaaa tctcgaccgg atccctgggg tcgggcggga cggcgcaagg
     1021 cggtaatatc ttcttcgcgc agaaagcccg ggaaatgctg catgctgtta gtagcgatga
     1081 gtcggaattc ttcctgcgca tggaagacaa ggaaaaaaag caattggccg accttcgggc
     1141 gagcttcctc gaaacgtcaa agaacatcag ggacatcaac gagaaatata cgcacaaacg
     1201 ccaggagcag gattccgccg acgggaacaa attcgatcaa tttgaaggag acgacagtca
     1261 tgctacgaaa ttgctccggc aattcaatga acaggcctgc aaggacggga agaagctggt
     1321 ggatgactat tacaatcatt acaaccggat cctcgaagac ctggcgcatg aatggatcag
     1381 ccgcgagacg tacttcacca atgagatcgt ctactacagc aaatatgcga gcctcgatga
     1441 caatgaatat gagatcaaga aggcgggcgc tgtcgcccat ttcatcaacc tgctcggaat
     1501 aagtgggaac gcatcgccgg tggattttta tagtgacgcc tccggttata tggccggcaa
     1561 ccttctttgc aaggagccgc cggaaaaaaa accgggcggt gggaaattgc aggactacga
     1621 tgacatccat tgcgacagcc atatctcctt gagcatcccc ggcgtcgtaa agtcgaaatg
     1681 ggattgcaat accgaaaccg acgagtggtc catcggtcct tccagtggga aatacaagga
     1741 gaacctcaac accggccagt tcaacgtcca cggggaactg ggggccagca ccaagatcag
     1801 ctccctgtcc gccgggccgc tcaaggtggg ggcctcggcc ggcgccgggg tcttcgtgga
     1861 ggctaccaac accggtatca ccgacgtggg cgtgaccgca cacgtggacg taaaactagg
     1921 cggcagtgtc gacatcccgc tgggcccgaa cagcgacggc aaggaagact cccattccgt
     1981 aggcaagtcc gtcaacctcg ccggcgccga agtccgcgtc ggctggaact cagggccctc
     2041 cctcacagga aaaggcgcct tgtcggggat atccataaaa tgaggatggg agatccgatc
     2101 ccgctactta atcaaatcct ttcttacaag cagagggtcg gcggttcaaa cccgtcaact
     2161 tgactaacta tttttcaaaa actggacttt ggccttcgga tcctccggcg ttatcctctt
     2221 agtcaccaga ttccagtttt tctttcaata cctgtagctc gcgttttctt atcttgttta
     2281 cgcgatctcc ttttcggcca atataaaacg caaggacgta cgaaatcagt attactgcgc
     2341 ttacctcaag taatt
//
LOCUS       DATGFG010000367         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_967815, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000367 DATGFG010000000
VERSION     DATGFG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967815"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="VKQ52_06985"
     CDS             complement(<1..1907)
                     /locus_tag="VKQ52_06985"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HLZ86963.1"
                     /translation="MKKALIAIALLSWVLPVLAQGPRTDTSSSFHDIEAHRVQLPNGW
                     SLTPAGTSLPLGDLPLNIAVSGSQHYAAVTNNGQSIQTIQLFDARADRQLDTVVIPKS
                     WGGLAFSEDEQSLYASGGNDNWILQYAIREGRLVTMDTFRLGASWPNRISPAGLVVDS
                     RRQLLYVVTKEDNSLYVVDLRAHVVLHRWPLSGEGYTCLLSPDRRELYVSVWGGDKVM
                     VYNTVTAGFTDSIPVGDNPNDLCLSRKGRWLYVANANDNSVSVIDVAARKVVETLNAA
                     LYPNAPSGSTTNGVTLSSDEKTLYVANADNNCLAVFDISQPGRSVSKGFIPTGWYPTC
                     VRAIGKKLYVANGKGFSSYSNPHGPNPYGVGQTVSLHAGDTQRVVKVQYIGGGLMMGK
                     LSIIPFPSASQLAVYSQAVYHNTPYKKENELVVAGEEGNPVPRRVGDASPIKHVFYII
                     KENRTYDQVLGDLPQGNGDTSLVLFGERVTPNQHLLAGEFVLLDNFYVDGEVSSDGHN
                     WSMGAYATDFLEKGWPTMYGRRGGSEISSGYRKTGDNKGGYIWDQAARSGVTYRTYGE
                     FADRGRANIPVLRDHFSPTFSSLDMHVRDTVRARQWASEFDSLVAANALPQLMTVRFG
                     NDHTEGTAANRP"
     gene            complement(2074..>2336)
                     /locus_tag="VKQ52_06990"
     CDS             complement(2074..>2336)
                     /locus_tag="VKQ52_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86964.1"
                     /translation="VDMVHPNYPATQFVLEKFTQQCLDAASRTLLEEVKKIVIARKHK
                     PFQPTTQAHRRFLQEQYAKAAALAEQYPFLDLEEELRYFSGR"
BASE COUNT          541 a          816 c          519 g          460 t
ORIGIN      
        1 ggccggttcg ccgccgtccc ctccgtatga tcattgccaa accgcaccgt catcagctgc
       61 ggcaacgcat tcgccgccac cagcgaatcg aactcgctcg cccactgccg cgcccgcacc
      121 gtatcccgca catgcatatc cagcgacgaa aacgtcggcg agaaatggtc ccgcaacacc
      181 gggatattcg cccggccacg gtcggcaaac tccccatagg tccggtacgt aaccccgctt
      241 cgcgccgcct gatcccagat ataccccccc ttgttgtccc ccgtcttacg ataaccggag
      301 gatatctcac tccccccacg ccgcccatac atcgtcggcc accccttctc caaaaaatcc
      361 gttgcatagg cccccatact ccagttatgc ccatccgaac tcacttcgcc atccacataa
      421 aaattatcca gcaacacaaa ctcccccgcc agcaaatgct ggttgggcgt gacccgctcc
      481 ccaaaaagca caagcgaagt atccccgttg ccctgcggca gatcaccgag cacctggtca
      541 tatgtcctgt tctccttgat aatataaaac acatgcttta tcggcgaagc gtcacccacc
      601 ctccgcggca ccgggttccc ctcctccccc gcaaccacca gctcgttctc cttcttatac
      661 ggcgtgttgt gatacaccgc ctgcgaatag accgccagct gcgaagccga agggaagggg
      721 atgatactca gcttccccat catcaaccca cccccgatat attgcacctt caccacccgc
      781 tgcgtatccc ccgcatgcaa actcaccgtc tgccccaccc catacggatt gggaccatgc
      841 gggttggaat aggacgaaaa ccccttcccg ttcgcgacat acagtttctt ccctatcgcc
      901 cgcacacagg tgggatacca ccccgttggg ataaaccctt tcgacacact cctccccggc
      961 tggctgatat cgaaaacggc aagacaattg ttatcggcat tggctacata caaggtcttc
     1021 tcatcagaac taagcgtcac cccattcgtg gtggacccgc tcggcgcatt gggatacaag
     1081 gccgcattca gcgtctccac caccttccgc gccgccacat cgatcaccga cacactattg
     1141 tcattcgcat tggccacata taaccaccgg cccttgcggc taagacaaag atcattggga
     1201 ttatccccca ccgggatact atccgtaaac cccgccgtca cggtattata gaccatcacc
     1261 ttatcccctc cccacacgga aacatacagc tcccgccggt cgggtgacaa cagacaggta
     1321 tacccttccc cactcaacgg ccaccggtgc aggacgacat gcgcccggag atccaccaca
     1381 tacaggctat tgtcttcctt cgtaaccaca tacaacaact gccgcctcga gtcaaccaca
     1441 agccccgccg gcgatatccg gttgggccag gacgctccca gcctgaacgt atccatcgtc
     1501 accagcctgc cctcacggat cgcatattgc aggatccagt tatcattccc cccggaagca
     1561 tacaacgact gctcatcctc gctaaaagca agcccgcccc aggacttcgg gatcaccacc
     1621 gtgtccagct gccggtccgc ccgcgcatca aaaagctgga tcgtctggat actctgcccg
     1681 ttattggtca ccgccgcata atgctgagag ccgctcaccg cgatattcaa cggcaaatct
     1741 ccaagcggca ggctggtccc ggccggcgtc agtgaccatc cattcggcaa ctgcacccgg
     1801 tgagcctcga tatcatgaaa ggaagaagag gtatccgtgc ggggtccctg cgcgaggaca
     1861 ggaagaaccc aggatagcag ggctatggcg atcagggctt ttttcatgac ttgaattgta
     1921 ggagacaaaa ctaccgatgg cgcaccggag aataaacccc ggcatgttaa tagactatta
     1981 agagaatctg aacctctgcc acccatccac ccatggcgcc ttcggcatcg gcacggccgt
     2041 taaactggca tatcccctct accgcccgct aatctaccgg ccgctaaaat agcgtaactc
     2101 ctcttccagg tcgagaaaag gatattgctc cgccagcgca gcggctttag catactgctc
     2161 ctgtaaaaac cgccggtgcg cctgcgtagt gggttgaaaa ggcttgtgct tgcgggcgat
     2221 cacgatcttt ttcacttctt caagcagcgt acgcgatgcc gcgtcaagac attgctgcgt
     2281 aaatttctcg agcacgaatt gcgtcgccgg gtaattggga tgcaccatat ccacgt
//
LOCUS       DATGFG010000368         4578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1479814, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000368 DATGFG010000000
VERSION     DATGFG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479814"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VKQ52_06995"
     CDS             <1..544
                     /locus_tag="VKQ52_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86965.1"
                     /translation="DLLLSVYRFLGLDYPKFYKMDQLSKLGWLASEILLQHEAGIGSY
                     PPEEIGLVLSNANSSLDTDNKYWETVKAVASPAVFVYTLPNIMMGEISIRNGFKGENG
                     FFIFDRFDAAFLEQYVGYLFDTQLLTVCICGWVDLLDDEYQAVLFLVANGSGDPAANG
                     AGNLIFSKENLDMIYKEAQQ"
     gene            545..1762
                     /locus_tag="VKQ52_07000"
     CDS             545..1762
                     /locus_tag="VKQ52_07000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HLZ86966.1"
                     /translation="MATNKYNHLPLYIAGMGVISAIGNNVEECLDALEHERAGMGRID
                     HLQTIHRDQIPVAGVPASNEELASLTGVSPLVSRTALLSLVAATEALASSGLETGSHW
                     RIGMVAANTVGGMDKTEHFFRSFLENSQKGRLRDVVGHECGTSTEWVADRLGIRSYRT
                     TINTACSSSANALAFAARLIRHGMLDVVIAGGADSLTTFTLNGFNTLMILDREYCMPF
                     DEDRKGLNLGEGAGYLVLVSEAVAATLTRAPTCRLAGFCNRNDAYHQTASSPDGRGSF
                     LAMQGALENSGLQPADIDYINLHGTGTQNNDLAEGLAIKRLFDPHYPKMSSTKSFTGH
                     TLGASGGIEAVFSALAVQQDIIYPNLRFRTAMKELPFTPETRLIKDTPVKHVLSNSFG
                     FGGNCSSLIFSKR"
     gene            1764..2744
                     /locus_tag="VKQ52_07005"
     CDS             1764..2744
                     /locus_tag="VKQ52_07005"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HLZ86967.1"
                     /translation="MTIYIRSMATISPCAAPGDHPSQTEPVNPSGNRLRAIEPDYTAF
                     IDAKQIRRMSRIVRMGVAAAVRCLREADEPMPGAIVTGTAYGCLEDTGIFLDRLIRQK
                     EEMLSPTSFIQSTHNTIGAQIALMLQCTHYNNTFVHRGFSFESALLDAILLLEEGSAE
                     TVLVGGVDETTEASHAILERFGLYRRAVAGEGAAFFLLSNRSSGKDRGQLEGLHTFYR
                     PASVAVIEDTIRCFLADRSMTMEEVGLVLMGTDGGLAADETDSPLKEGIFRGKECVGF
                     KKLCGDYPTASSFALGLAAGMLAEEKTPKRILIYTHYLNIYHSLMLVSTC"
     gene            2738..3109
                     /locus_tag="VKQ52_07010"
     CDS             2738..3109
                     /locus_tag="VKQ52_07010"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86968.1"
                     /translation="MLRNDFYTVGKIEKEGLSIKTSLYLTVDHPLYQGHFPARPVVPG
                     VCMLQMIKELTELATGRALQLSTARTMKFLLPIVPADTPTLQVGITWQMPDPSSVEIN
                     ATLLKDNSPCFKFSGIFRIVD"
     gene            complement(3106..4152)
                     /locus_tag="VKQ52_07015"
     CDS             complement(3106..4152)
                     /locus_tag="VKQ52_07015"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HLZ86969.1"
                     /translation="MLYFHQAVCISPLPTFGSVDLTRPGVPQGNKLLVKEPAYEGIPA
                     NLVRRTGKAVRMGVGVSMPILRATPVPVSGIIIGTANGGTEDSFRFLQQIIDYKEDTL
                     APGSFVQSTANAAAAQISLLDKNKGYNLTHVHRGLAFEFALLDAVLLSEENKEAYYLL
                     AGIDETSGPNFMLDAMEGWHKKETLQAGGFYAPDSPGAIAGEGAAGFSVSGQGQNALA
                     RLAGVSTLHSNDPVSVRRRMEEFLQGCLPADTAIDLFLSGENGDCRLQKFYTACETGL
                     PAEVPIARFKHCCGEYPTASAFAVWLCCQLLSGMALPEHMVKRGKPAPFQTILLYNNY
                     KGLQHSFILLSVVR"
     gene            complement(4152..>4578)
                     /locus_tag="VKQ52_07020"
     CDS             complement(4152..>4578)
                     /locus_tag="VKQ52_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86970.1"
                     /translation="HPTSLSPTGEGPFLAMQGALASAGISPAGIDFINAHGTGTGNND
                     EVESKAMIRVFERPPAFASTKANTGHTLGAAAAIEAVYSLLCLDNQEIYPNLHFTEAI
                     PGTELRPVTVYRPYPLRHIMSNSFGFGGNCVSLIFSKPS"
BASE COUNT         1040 a         1292 c         1241 g         1005 t
ORIGIN      
        1 cgatcttctg ctttctgtct accggttcct cgggctggac tacccgaaat tctataagat
       61 ggatcagttg agcaaactgg gatggctggc ctccgagatc ctgttgcaac acgaagccgg
      121 gatcggctct taccctccgg aggagatcgg gctcgtgctc tccaatgcca attcaagcct
      181 ggataccgac aataaatatt gggagacggt gaaggccgtt gccagcccgg cagtatttgt
      241 ttacaccttg ccgaatatca tgatggggga gatcagcatc cggaatggtt ttaaagggga
      301 gaacgggttt tttatcttcg atcggttcga cgcggccttc ctggagcaat atgtcgggta
      361 ccttttcgac acacaactgc tgaccgtctg catttgtggc tgggtcgatc tgctggacga
      421 cgaataccag gctgtattat tcctggtcgc gaacggctcg ggtgacccgg cggcaaacgg
      481 ggccggaaac ctgatttttt caaaggaaaa tttggacatg atctataagg aggcgcaaca
      541 ataaatggca acgaataaat ataaccatct tcctctctat attgccggca tgggtgtcat
      601 ctccgcaatc ggcaacaacg tggaagaatg cctcgatgcc ctggagcatg aacgggccgg
      661 catggggcgg atcgaccatt tgcaaaccat tcaccgggat cagatcccgg ttgccggtgt
      721 ccctgcttcc aatgaggagc tggcttccct gacaggcgtc tcacccctgg taagccgcac
      781 cgccctatta agtcttgtcg ccgctaccga agccctggcc tcttccggcc tggagaccgg
      841 ttcccattgg cgtataggca tggtcgccgc caatacggtg gggggtatgg acaagaccga
      901 acattttttc cgttccttcc tggaaaactc ccaaaagggc agattacggg acgtggtcgg
      961 tcatgagtgc gggaccagta ccgaatgggt ggccgatcgc ctcggtatcc gatcctatcg
     1021 tacaacgatc aatacggcct gctcctcctc ggccaacgcc ctggcctttg ccgccaggct
     1081 gatccggcat ggaatgctgg atgtggtgat cgcagggggg gcagattcgc tgacaacctt
     1141 caccctcaac ggattcaata ccctgatgat cctggacagg gaatattgca tgcctttcga
     1201 tgaagaccgg aaaggcctca atctcggcga aggggcggga tacctggtcc ttgtatccga
     1261 agccgtcgcg gctacgctga ccagggctcc cacctgccgg ctcgccggct tctgcaacag
     1321 gaatgacgcc tatcaccaga cggcttcttc ccccgacgga aggggttcct tcctggctat
     1381 gcagggcgcg ctcgaaaaca gcggactaca acccgccgac atcgattaca tcaatttgca
     1441 tggcaccggc acgcagaaca acgacctggc agaagggctg gctataaaac gattattcga
     1501 tccccactat ccgaaaatga gttccacgaa atccttcact ggtcataccc tgggcgcaag
     1561 cggggggatt gaagccgtct tttccgccct tgcggttcaa caggacatca tttaccccaa
     1621 cctgcggttc cggacggcta tgaaagagct gcctttcacc cctgaaaccc ggctgataaa
     1681 agacacaccg gtgaaacatg ttctgtccaa ttcattcggc ttcggcggca attgttcatc
     1741 tctaatcttt tcaaaacgat aagatgacca tctatatccg atcaatggcc actatctctc
     1801 cctgtgctgc cccgggcgat cacccgtcgc aaacggagcc ggtgaaccct tccggcaacc
     1861 ggctaagggc catcgagccg gattatacgg cgttcatcga tgcgaaacag atccgcagga
     1921 tgagccgcat cgtcaggatg ggagtggccg ccgccgtccg ttgtctcagg gaagcagacg
     1981 agccgatgcc gggggccatc gtaacgggca ccgcgtatgg ctgcctggaa gataccggca
     2041 ttttccttga ccggctgatc cgtcaaaagg aagaaatgct ctcacccacc tcctttatcc
     2101 aatcgacgca taataccatc ggcgcccaga tcgcactgat gcttcaatgc actcattaca
     2161 acaatacgtt cgttcatcgc gggttctcat tcgaaagcgc cctcctggat gccatcctcc
     2221 tcctggagga aggatctgcc gaaaccgttt tggttggggg cgtggatgaa acgacggagg
     2281 ccagccatgc catccttgag agatttgggc tttaccggcg ggcggtcgcc ggcgaaggag
     2341 ctgccttctt ccttctttcg aaccggtctt ccgggaaaga ccgggggcaa ttggaaggac
     2401 tccatacttt ttatagacct gcatcggtgg cggtgatcga agatactatc cgctgcttcc
     2461 tagcagaccg gtccatgacg atggaagagg tcggcctggt attaatgggc acggacggcg
     2521 ggctcgcagc agatgaaaca gacagtccgc tgaaggaagg gatctttcgc ggaaaggaat
     2581 gcgtcggatt caaaaagctt tgcggggatt acccaacggc ttcctcgttt gcgcttggac
     2641 tggcggccgg catgctggca gaagagaaaa cgccaaaaag aatattgatc tatacgcact
     2701 acctgaacat ctatcattcc ctcatgctgg tctcgacatg ctaaggaacg acttctatac
     2761 tgttggtaag atagaaaagg aaggactctc gataaagacc tccctttatc ttaccgtgga
     2821 ccatcccctt taccagggcc atttcccggc gcggccggtg gttccgggtg tctgcatgct
     2881 gcagatgata aaggaattga ccgaattggc gacaggccgc gccctccaac tctccacagc
     2941 ccggacaatg aaattcttac tgccgatcgt tcctgcggac actcccacct tgcaggtcgg
     3001 gatcacctgg caaatgcccg atccctcatc cgtagagatc aacgctaccc tgctgaaaga
     3061 caactccccc tgttttaaat tttcggggat tttccggatc gttgattaac gcaccacgga
     3121 caacaggata aaactatgct gaagcccctt ataattgtta tagaggagaa tggtttgaaa
     3181 tggggctggc tttccccgct tgaccatatg ctccggcagt gccattcccg aaaggagttg
     3241 acagcagagc cataccgcaa aggcggaagc cgtagggtac tctccacagc aatgtttgaa
     3301 acgggcgatc ggaacctctg ccggcagtcc tgtttcgcag gcggtataaa atttttgtaa
     3361 ccggcaatct ccattctcac cgctcaggaa aagatcgatg gccgtgtccg ccggtaagca
     3421 gccctgcaag aattcttcca tgcgccgccg gacagaaacg gggtcattac tgtgaagggt
     3481 cgagacccct gccagccggg caagggcatt ttgaccctgg cccgacacgg agaaacccgc
     3541 cgccccctcc cctgcgatcg caccgggaga atccggagca taaaacccgc ccgcctggag
     3601 ggtctccttt ttatgccacc cttccattgc atcgagcata aaattcgggc cggacgtctc
     3661 atcgatcccg gccaataaat agtaagcctc tttattctct tctgataaaa ggactgcatc
     3721 cagtaaggca aattcgaaag ccagcccgcg gtgtacgtgg gtcaggttat accctttgtt
     3781 cttgtccagc aggcttattt gcgccgccgc tgcattggcg gtgctttgga cgaaacttcc
     3841 cggggcgaga gtatcttcct tgtagtctat gatctgttgc aaaaaacgaa aactatcttc
     3901 cgtgccgccg ttggcggtgc cgatgataat acctgaaacc ggaacgggtg tcgcccgcag
     3961 aatgggcatg ctcaccccca cccccatccg tacggccttt cccgtcctgc gcaccagatt
     4021 cgcggggatg ccttcgtaag caggctcttt caccagcagt ttatttccct gtggcactcc
     4081 cggccgggtc agatcgacgg atccaaacgt aggcaggggg gagatacaaa cggcctggtg
     4141 gaagtagagc attatgaggg tttggaaaag attaatgaga cacaattgcc gccgaatcca
     4201 aaagaattgg acatgatatg acgcagtggg taaggccggt agaccgtcac cggccgcagc
     4261 tccgtgccgg ggatagcctc ggtgaaatgc aggttggggt agatctcctg gttgtcgaga
     4321 cacaacaggc tgtaaaccgc ttcaatggct gctgccgcgc ccagggtatg accggtgttc
     4381 gctttcgtgg aagcaaaggc cggaggccgt tcgaacaccc ggatcatcgc cttgctttcc
     4441 acctcgtcgt tattcccggt gcccgtacca tgggcattga taaaatctat tcctgccggg
     4501 gatatcccgg ccgaagccag ggcgccctgc atggccagaa aaggaccttc tcctgtggga
     4561 gaaagagacg tcgggtga
//
LOCUS       DATGFG010000369         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1408406, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000369 DATGFG010000000
VERSION     DATGFG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408406"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VKQ52_07025"
     CDS             <1..318
                     /locus_tag="VKQ52_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86971.1"
                     /translation="TLLVGGRDNTTIRGRVVPGRWTLDKSFDGNGNTRPKGIVYIVTG
                     AGGQELYNPEQTADPDSWQKFTDKFLSTQHSISVLDIDGRTFSLRQMAADGQQIDSFR
                     ITK"
     gene            385..1548
                     /locus_tag="VKQ52_07030"
     CDS             385..1548
                     /locus_tag="VKQ52_07030"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF010611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HLZ86972.1"
                     /translation="MVRLPIYLDYNSTTPCDPRVLDAMLPYFTQKFGNAASRSHAFGW
                     EGEEAVEIAREQVARLIGAEPKEIVFTSGATEGDNLALKGVFEAYASKGDHIITVVTE
                     HKAVLDTAHHLEKLGGGITWLPVDPKGLIDLAALEAAIRPTTILIAVMYANNEIGVLQ
                     PVKAISEIARRHGVLFFTDATQAVGKIPVDVNADGIDILTLSAHKLYGPKGIGAIYVR
                     RRSPRVRLSAQMDGGGHERGMRSGTLNVPGIVGLGKASELAAMEMGEEAARVGRLRNR
                     LEAGLLQIEGARVNGSREHRLPHTTNISFPGVDGEALLAGLGKDVALSSGSACTSASM
                     EPSYVLKALGIADDLAHSSLRLGLGRFTTEEEVDYAIGRITQTVEKLREKAFK"
     gene            2009..>4238
                     /gene="gldG"
                     /locus_tag="VKQ52_07035"
     CDS             2009..>4238
                     /gene="gldG"
                     /locus_tag="VKQ52_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HLZ86973.1"
                     /translation="MWAVCKKELRQFFSSLLGYIAIIVFLLLNGLLLFVFPDTDILSF
                     GYATLDKFFELAPWILLLLTPAITMRSFSDEFRMGTYETLQTVPLTRGRLILGKYLAS
                     LVVVLIALLPTLVYFLCIQRLSGQGGIDTGATIGSYLGLVFLAGVFTAIGIWCSSFTA
                     NSVVAFIIAAFACFILYSGFGAISALPVFSAGADYYIGMLGIDFHYRSISRGVVDCRD
                     LLYILSVVFLFLYLTGRHLAAKQLAGKRRGAWLRPVGLVVVLALVNGLASHLHFRLDL
                     TQEHRYTLSAPTKEMLGRLDDDLQVDFFMAGDLKAGIRKLAKSTDDLLHEFNEYCDGR
                     IRVHAFDPLATLDDSAKVNFLDSMRRMGIQPMTQVAQAKRGEEESQRVVIPAAIVRYK
                     DRIYPVNLLTGVQERGEGQPQEQLYTNAETLLEYKFGSAIDKVTQKEVPRVGYVLGNG
                     EPLDYRVYDLIEDLRHNYRFGIVPLDSVAVIPAEYKALVIVKPTQRFTQREKLTLDQY
                     VLHGGQIIWAIDNLHAEKDSLTQAQGTIAYDRGLDLEDLFFRYGVRVNQDLVEDAGQC
                     ANLSLVVGMQGDKPQIEALKWPYYPLLNGSLTHPISKNLDPVLSEFANSIDTVKAPGI
                     KKTVLLQTSANSRRVSTPALITLDVLKYKDDPRMFPESNIPVAMLLEGKFRSLFANRI
                     SSAQADSLANVSHTPFLPEATTPSRVIVCADGDIFMKEVTQRGPLPLGYSADNDYKFA
                     NQD"
BASE COUNT          919 a         1292 c         1186 g          841 t
ORIGIN      
        1 accctattgg taggcggcag ggacaatacc accatccgcg gccgcgtggt cccgggcagg
       61 tggacgctgg ataaaagctt cgacggcaac ggcaataccc ggccgaaagg gatcgtctac
      121 atcgtcaccg gcgccggcgg ccaggaattg tacaaccccg aacagacggc cgaccccgat
      181 agctggcaga agttcaccga caaattcctg tctacccaac actcgatcag cgtgctcgac
      241 atcgatggcc gcaccttctc gctgcgccag atggccgccg acggacagca gatcgattcg
      301 ttccgcatca caaaataagg accggccccg acccccggcc ccggctgccg gcgatcggat
      361 aacctggagt cgtacctttg caccatggta cgcctgccca tctatctcga ctacaactcc
      421 accaccccct gcgacccccg ggtgctggac gccatgctgc cctattttac gcagaagttc
      481 ggcaacgccg ccagccgcag ccatgccttt ggctgggagg gcgaagaggc ggtggagatc
      541 gcgcgcgaac aggtggcgcg gctcatcggg gcggagccaa aggagatcgt cttcacctcg
      601 ggggcgacgg agggcgataa tctcgcgctg aaaggggtct tcgaagccta tgcctcaaaa
      661 ggcgatcata tcattaccgt cgtcaccgaa cacaaggcgg tgctggacac ggcccaccat
      721 ctcgaaaagc tgggcggcgg gatcacctgg ctgcccgtcg atccgaaagg actcatcgat
      781 ctggccgcgc tggaagccgc catccggccc accaccatcc tgatcgcggt gatgtacgca
      841 aataatgaga tcggcgtcct ccagccggta aaggccatca gtgagatcgc aaggcggcac
      901 ggcgtcctct tcttcaccga cgccactcag gccgtgggca agatcccggt ggatgtaaat
      961 gccgacggta tcgatatcct caccctcagc gcccataagc tctatggacc caagggcatc
     1021 ggcgccatct atgtccggcg acggagcccc cgcgtgcgac tgtcggcgca aatggacggc
     1081 ggcggacacg aacgggggat gcggagcgga acgctgaatg ttcccggcat cgtcggcctg
     1141 ggaaaggcga gcgagcttgc cgcaatggag atgggagagg aggctgcccg ggtcggccgc
     1201 ctgcgcaatc gcctggaagc cggtctgttg cagatcgaag gcgcccgggt caacggaagc
     1261 cgggaacacc gcctgccaca taccaccaat atctccttcc ccggcgtcga cggcgaggcg
     1321 ctgctcgcgg gactgggtaa ggacgtggcc ctctcctcgg gttcggcctg tacctcggcc
     1381 tccatggaac ccagctatgt cctgaaagcc ctgggaatcg ccgacgacct ggcccatagc
     1441 tccctgcgcc tgggcctcgg ccggttcaca acggaagaag aagtggacta cgccatcggc
     1501 cggatcaccc agaccgtgga gaaattgcgg gaaaaagcgt tcaaataatg catatatatt
     1561 ttattgcata cgataatagc aatttttcat aatatgaaat ctggcctttc agggccgctg
     1621 ggaaggacgc ccggaagtgc ccgcttgttg acccgtccat cccattcggg aggcggcggg
     1681 aaggacgaaa aaagaggtga aaaggacgag cgggaaatgg gctaaaacaa aaaacccgcc
     1741 tgactaaggg cggatttttt catgcagttg gtttcggttg aatgtcttga ttgacctcac
     1801 cataaatata ttatttcgaa ggatcggctt ggcggcaaga gtatgcgaat atagggatat
     1861 ctttcatact acccattttt cttcagtcta aatttagagg attttttcac attttattta
     1921 ttctaatgta cgtgatcgcc ccataaaagc ctgttttaca caataacaaa ccgttttcga
     1981 acgattataa agccatactt tcgtgtctat gtgggcagta tgtaaaaaag agcttcgcca
     2041 attctttagc agtcttctcg gttatatcgc catcatcgtc ttcctgctcc tgaacgggct
     2101 gctgctcttc gtttttcccg ataccgacat cctgtccttt ggctatgcca ccctggacaa
     2161 attcttcgag ctggccccct ggatcctgct cttgctgacc cccgcgatca cgatgcgcag
     2221 cttctcggat gagttccgca tgggaacata cgagaccctg cagaccgtcc ccctcacccg
     2281 gggccggctg atcctcggga aatacctcgc cagcctggtc gtcgtactga tagccctgct
     2341 cccgaccctg gtctacttcc tttgtattca gcggctttcc ggccagggcg gcatcgatac
     2401 gggagcaacg atcggcagtt acctggggct ggttttcctg gccggggtgt tcaccgccat
     2461 cggcatttgg tgcagcagct tcaccgccaa ttccgtcgtt gcctttatca tcgcagcctt
     2521 tgcctgtttt attctctata gcggttttgg agccatcagc gccctgcccg tcttcagcgc
     2581 cggcgcggac tattatatcg gcatgctcgg catcgatttc cactatcgca gcatcagccg
     2641 cggggtggta gactgccgcg atctgctcta tatcctcagc gtcgtcttcc tcttccttta
     2701 ccttaccggg agacacctgg cggcaaagca actggccgga aagcgcaggg gagcctggct
     2761 gcggccggtg ggcctggttg tcgtactggc gctcgtcaac gggttggcct cccatctcca
     2821 ctttcgcctc gatctcaccc aggaacatcg ctataccctg agcgcaccga caaaggagat
     2881 gctgggccgc ttggacgacg atctccaggt ggactttttc atggccggcg acctgaaagc
     2941 cgggattcgc aaattggcca aaagcaccga cgatctcctc cacgaattca acgaatactg
     3001 cgacggccgg atccgtgtcc atgccttcga tcccctggcc acactagacg acagcgccaa
     3061 agtgaatttc ctcgattcga tgcggcggat gggcatccag ccgatgaccc aggtggcaca
     3121 ggcgaaaagg ggcgaagaag agagccagcg cgtggtgatc cccgcggcga tcgtccgcta
     3181 caaagaccgc atctatcccg tcaacctgct gacgggcgtg caggagcgcg gcgaaggcca
     3241 gccccaggaa caactctata ccaatgcgga gacgctgctg gaatataaat tcggatcggc
     3301 gatcgataag gtcacccaaa aggaggtccc acgggtcggc tatgtgctgg gcaacggcga
     3361 accactcgat taccgcgtat acgacctgat cgaagacctc cggcataatt accgttttgg
     3421 catcgtcccc ctggacagcg tggccgtgat ccccgccgaa tacaaggccc tcgtgatcgt
     3481 aaaacccacg cagagattta cccaacgcga gaaactcacc ctcgaccagt acgtactcca
     3541 cggcggccag atcatctggg cgatagataa tctacatgcg gaaaaggaca gcctgacgca
     3601 ggcacaggga accatcgcct atgatcgcgg cctcgacctc gaagacctgt tcttccgcta
     3661 tggggtccgg gtcaaccagg atctggtgga agacgccggg caatgcgcca acctttccct
     3721 ggtggtgggg atgcagggcg acaaaccaca gatcgaagcg ctgaaatggc cctactatcc
     3781 cctgctgaac ggcagcctta cccatcccat ctccaaaaac ctcgaccccg ttctatcgga
     3841 gtttgccaat agcatcgaca cggtaaaggc gccggggata aagaagaccg tattgctcca
     3901 gacctcggcc aattcacggc gggtgagtac gccggcgctg atcacgctgg atgtgctgaa
     3961 gtataaggac gacccgcgga tgttccccga gtcgaatatc cccgtggcga tgctgctgga
     4021 aggaaaattc cggtcgctgt tcgccaaccg gatctcgtcg gcgcaggcgg acagcctggc
     4081 gaatgtctct cacaccccct tcctgcccga ggcgacgacg ccttcgcggg tgatcgtatg
     4141 cgcggacgga gatatcttta tgaaggaggt gactcaacgc ggtccgctgc ccctcgggta
     4201 tagcgcggac aatgactaca agttcgccaa ccaggatt
//
LOCUS       DATGFG010000370         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_505521, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000370 DATGFG010000000
VERSION     DATGFG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505521"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VKQ52_07040"
     CDS             complement(<1..349)
                     /locus_tag="VKQ52_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86974.1"
                     /translation="MNARLVAADIFVFIVYFILVSLYGYWIYRKKGKKATNTKEFFLA
                     EGSLTWWAIGASIIASNISAEQFIGMSGDGFFAGIAVAVYEWLGAIALIIVAIFFMPV
                     YLKNKIYTMPQFLK"
     gene            complement(501..1382)
                     /locus_tag="VKQ52_07045"
     CDS             complement(501..1382)
                     /locus_tag="VKQ52_07045"
                     /inference="COORDINATES: protein motif:HMM:NF017006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HLZ86975.1"
                     /translation="MSTNADRTEANPLSSLDVWEDDLLERYPDPESIAKGKTTEEYRN
                     YESPARDSVREFYRLNHTYQTYDFVRGKQEEFLKFDKKEMSVWDAFAFLNQLVDDSDP
                     DTDLDQFQHLLQTSEAIRADGHPDWMVLAGLFHDMGKTLCLFGEPQWAVVGDTFPVGC
                     AYSEKIVYPEFFVHNPDYNHGVYSTKYGVYSHGCGLRNVNMSWGHDEYVYQMMKDHLP
                     EPALYMLRYHSFYSQHRENGYDHLMDEHDREMFKWVNLFNPYDLYSKNPVPPNWNELK
                     PYYEDLVAKYLPATLKF"
     gene            1474..>2485
                     /locus_tag="VKQ52_07050"
     CDS             1474..>2485
                     /locus_tag="VKQ52_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLZ86976.1"
                     /translation="MGNVTIRILAGKLQLSTATVSKALTDSHEISEKTKQRVLALAAE
                     LNYTPNPYASGLRKKKSRTIAVILPEVADSFFAQAIDGIESIAQEKGYHVLIRLTHES
                     FEKEKSILQDLRGGRADGVLMSVSAATEDSSPIRELQDKGIPLVFFDRVSEDISTAKV
                     VTDDQESAYEATGHLIRNGCRRIVLLSASTTLSIINQRIEGYRKALSDHQMPLRDSDI
                     VTCSPDPVVNDKILKSLLSGGDPPDGFIATVEKLVTPVYLACRDLQLRIPDQVKVIGF
                     SNLPSAPLLSPSLTTITQPAFEMGKAAASLLFKALERDQFTLAEHNLVIPSILMVRGS
                     TGG"
BASE COUNT          658 a          629 c          606 g          592 t
ORIGIN      
        1 tcttcaggaa ttgcggcatg gtgtatatct tgttcttcag atacaccggc ataaagaata
       61 tcgccacgat gatgagggcg atagcgccca gccattcata aaccgctacc gcaataccgg
      121 cgaaaaagcc gtctccgctc atgccgatga actgctccgc ggaaatattg gaggcgataa
      181 tggaggcgcc gattgcccac caggtgaggg agccttcagc caggaaaaat tctttcgtat
      241 tggtagcctt cttccccttt ttgcggtaga tccaataacc atacaaggaa acgaggataa
      301 aatagacgat gaatacgaag atgtcggcgg caaccaatcg agcgttcata agcaatatga
      361 tctgttattc catttaagca tatcaaaaaa ccaggcactc ctgaaaagtc gcctggtttt
      421 atgcaaggac gaatatagta aattttcaat gacctctcag caaggccatc ggcctaaggc
      481 ggtccgtcgc cttttggtcg tcaaaatttg agggtcgcgg gcaggtattt cgccacaaga
      541 tcctcatagt agggcttcaa ctcgttccag ttcggcggaa cggggttctt ggaatacaag
      601 tcataggggt tgaacaaatt cacccatttg aacatttccc ggtcatgctc atccatcaaa
      661 tgatcatatc cattctcccg gtgctgggaa taaaaggagt gataacgcag catgtacaaa
      721 gctggctccg gaagatggtc cttcatcatc tggtatacat attcatcatg cccccaggac
      781 atattgacat tgcgcaatcc gcatccatgg gaataaactc catatttcgt gctgtatacc
      841 ccgtgattat agtctggatt gtgaacgaaa aattcaggat acacgatctt ttccgaatag
      901 gcgcagccta ccgggaaagt atccccgacg acagcccatt gcggttctcc gaaaaggcaa
      961 agggtcttgc ccatgtcatg gaacaaaccc gccagaacca tccaatccgg gtgcccgtcc
     1021 gcacggatgg cctcggaagt ctgcaataaa tgctggaact ggtcaaggtc cgtatccggg
     1081 tcggaatcgt ctaccaattg gttcagaaat gcaaaagcgt cccaaacgga catttccttc
     1141 ttatcgaact tcaggaattc ctcctgtttc ccacggacga aatcataagt ctgataagta
     1201 tggttgaggc gataaaattc acggacggaa tcccgggcag gggattcata gttcctgtat
     1261 tcttccgtag tcttgccttt ggcaatcgac tccgggtccg gatagcgttc aaggagatcg
     1321 tcctcccata catccaggga ggaaagagga ttagcttcag tacggtctgc atttgtgctc
     1381 atggcatttt gatttaatcg ttacggggta aattaccgat ctttttaacg atcttacata
     1441 caatgacata actttattta cttaaccgat ttcgtgggaa atgtaacgat caggatatta
     1501 gcaggtaagc tgcagctctc taccgccaca gtctcaaagg cgctgacgga cagtcatgag
     1561 atcagcgaaa agaccaagca aagggtattg gcgctggccg cggaattgaa ttatactccc
     1621 aatccatatg ccagcgggct gcgaaagaaa aaaagcagga cgatcgccgt cattcttccg
     1681 gaggtagccg atagtttttt tgcgcaggca atagatggca tcgaatccat cgcccaggag
     1741 aaaggctacc atgtattgat ccggctgacc catgagagct ttgagaaaga aaagtccatc
     1801 ctgcaggacc ttcggggagg cagggccgat ggcgtattga tgtcggtgag cgcggcaacc
     1861 gaggactcct ctcctatccg tgaattgcag gataaaggca tcccgctcgt attcttcgac
     1921 agggtctcgg aggatatatc gaccgcaaag gttgtcaccg atgatcagga aagcgcctat
     1981 gaagccaccg gccatttgat ccggaacgga tgccggcgga tagtactttt atccgcttcc
     2041 acgaccttgt ccatcatcaa ccagcgtata gaaggctata gaaaagcttt gtcggaccat
     2101 caaatgcccc tgcgggattc cgatatcgtc acctgttcac cggaccccgt tgtcaatgat
     2161 aagattctga aatcattact ctccggcggg gacccacccg atggctttat cgcgacggtt
     2221 gaaaaactgg tgactcccgt ctacctggca tgccgggacc tgcaattgcg catacccgac
     2281 caggtgaaag taatcgggtt ttccaatctt ccatcggcgc cccttctgtc cccttctctt
     2341 accacgatca cgcaaccggc atttgaaatg ggaaaggcgg cagcctccct gctattcaaa
     2401 gcgctggaaa gagaccagtt taccctggct gaacacaacc tggtgatccc ttcaatcctg
     2461 atggtgagag gatcgaccgg aggag
//
LOCUS       DATGFG010000371         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1468131, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000371 DATGFG010000000
VERSION     DATGFG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468131"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1358
                     /locus_tag="VKQ52_07055"
     CDS             90..1358
                     /locus_tag="VKQ52_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLZ86977.1"
                     /translation="MPDVIFNNQYWMYTVDPEAEEPVMLINTHIGYDPDEGFGCMGDM
                     FQRELLQLDALCIESNKKRIQVWINSPGGIVEDGMSIYSAILKTTTKVDTICVGLAAS
                     IAGVIFQAGRTRIMSDYAYLMYHNPYGSDDDKGMAAITDMIVIAIAARTGKPKEEIIK
                     LMNKTSWIGADEAVQTGLADKIEVSGELNKKRAPADANEAKNFYRESRAIVNKLTFKN
                     TTMADQFPFARIANKLGLHEMASADAIMEVLSGIMNKKDEDKKKAEDLEDAITKCKRD
                     MDDLKKEHDDLKKKHADLKAEYDEHMKKMEDKKKAEEEDRKKAEEEDKAKAEDKAKNL
                     VDTAAAQGKIPKTKEALETWKNKAIADYAGVESLLKTMGVSVKASGPVAKPETGKVAN
                     LEEAGKVYNMATEMAVRSAKYDEEFRNRRK"
     gene            1402..2430
                     /locus_tag="VKQ52_07060"
     CDS             1402..2430
                     /locus_tag="VKQ52_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86978.1"
                     /translation="MPEALVINDTSWSGYAASYMLTRPVVSADTIEKGCVMIQDDIRK
                     QWTLPRIEVSNFMQPRAATPVSQGTVTVDSAVLAPKDLMLYYEFDPRDYEAHFYAEQL
                     QPMLINRELPPTAEEFMMLQTMKRLNEFFENAFWRSRIMYNPAGLNINPTSKGAAATD
                     SAYYYFDGFIEKALASGSTIQVPSPVTLTSSNIVSTGFQPAYLLVPPAQLYKYGPKGL
                     KFMISYPDQQNYEFYMQTVTTFKNQDTTQTGINRYNGYDVVPLAGMPASTFFLGIGKP
                     DRDSNLWVGVNSAAEEGWIAADGRKAILELAHLQPNSELFFVKGLFKVDTQIGFTDQE
                     VVYTLQTA"
BASE COUNT          701 a          680 c          678 g          526 t
ORIGIN      
        1 agtaatttgc tgggcttttt cattttcttg cattagtttt ttccctttcc cccccttttt
       61 cccaaagttt acatcggaat atctcaaaaa tgcccgatgt tatcttcaac aatcagtatt
      121 ggatgtatac cgtcgatccg gaagcggaag agccggtaat gttgataaac acccacatcg
      181 gatatgatcc ggatgagggt ttcggttgca tgggggatat gttccaaaga gagcttttgc
      241 agctcgacgc tctttgcatc gaatcgaaca agaaacgcat tcaggtctgg atcaattcgc
      301 ctggcgggat cgtggaggat ggcatgtcga tttattccgc cattctaaag actacgacca
      361 aggtggatac gatttgtgtc gggttggcgg ccagtatcgc cggggtaatc ttccaggcgg
      421 gccggacccg gatcatgtcg gattatgcct acctgatgta ccacaatccg tacggatcag
      481 atgacgacaa gggtatggcg gccatcaccg atatgatcgt gatcgcgatc gcggcccgga
      541 cagggaagcc aaaagaggaa atcatcaaac tgatgaataa gacctcctgg atcggggcgg
      601 atgaggcggt acaaaccggg ttggccgata agattgaggt ctccggggaa ttgaacaaga
      661 aacgagctcc tgccgatgcg aatgaagcga agaatttcta ccgggaatcc cgggcaatcg
      721 taaataaact cactttcaaa aatactacta tggcagatca gtttcctttc gccagaatcg
      781 ccaacaaact cgggttgcat gagatggcct ccgccgatgc catcatggaa gtcctttccg
      841 gcatcatgaa caaaaaggat gaggacaaga aaaaggcaga ggacctcgag gatgccatca
      901 ccaagtgcaa gcgtgatatg gacgacctga aaaaggagca tgatgacttg aaaaaaaagc
      961 acgctgatct gaaggccgag tacgatgagc acatgaagaa gatggaggac aagaaaaagg
     1021 ccgaagaaga ggaccgcaaa aaggccgaag aagaggacaa ggccaaagcc gaggataagg
     1081 cgaaaaacct ggtcgatacg gctgccgctc agggcaaaat ccccaagacc aaggaggcat
     1141 tggaaacctg gaagaacaag gccattgcgg attatgccgg tgtggaatcc ctgttaaaaa
     1201 ccatgggagt cagcgtgaag gcttccggtc cggtcgccaa gccggaaacc ggtaaggtcg
     1261 ccaatttgga agaagccggc aaggtctaca acatggctac tgaaatggcc gtccgcagcg
     1321 cgaagtacga tgaggaattc cgcaaccgcc gcaagtagtc gaactacgaa ataattacat
     1381 aactcattaa aatacaacaa gatgcctgag gctttagtga taaacgacac cagctggtcc
     1441 ggctatgccg catcctacat gctgacccgg ccggtggtct ccgccgacac catcgagaag
     1501 ggatgcgtga tgatccagga cgacatccgc aaacaatgga cgctgccccg gatcgaagtt
     1561 tccaacttca tgcaaccccg cgccgcaacc ccggtctctc agggaacggt cactgtcgat
     1621 agtgcagtgc tcgccccgaa ggatctcatg ctctattacg agttcgatcc tagggattat
     1681 gaagcgcact tctatgccga gcaactgcag ccgatgctga tcaaccggga acttccgccg
     1741 accgctgaag agttcatgat gctccagacc atgaaacgtc tgaatgagtt tttcgagaat
     1801 gccttctggc gctctcggat catgtacaat ccggccggtc tcaacatcaa ccccaccagc
     1861 aagggtgccg ctgccacgga ttcggcctat tactactttg acgggttcat tgaaaaggcc
     1921 ctcgccagcg gcagcacgat tcaggttccg tcgccagtga cgctgacatc tagcaatatc
     1981 gtgagcacgg gcttccagcc ggcctacctg ctcgttcccc cggctcaact ctacaagtat
     2041 gggccgaagg gattgaagtt catgatctcc taccctgatc agcagaacta cgagttctac
     2101 atgcagacgg tgacgacctt caaaaaccag gatacgaccc agaccggcat caaccgatac
     2161 aatggatacg acgtcgttcc cctggctggt atgcccgcga gcacgttctt cctggggatt
     2221 ggtaaaccgg accgcgattc caatctctgg gtgggtgtga acagcgccgc cgaagagggt
     2281 tggattgccg ccgatggccg taaagcgatc cttgaactgg ctcacctgca gcccaactcc
     2341 gaactgttct ttgtgaaagg cctgttcaag gtcgacacgc agatcgggtt cacggatcag
     2401 gaagtggtct acacgctgca aaccgcataa ggacacattt aacgcaaaaa tattccgcaa
     2461 taaacgccta aaaatattcc aatggcaaca tcttcccgct tcggatcggc cctactgccc
     2521 aaccaggata aaaccggccg ggtggcaacg tttgacacgc agacgccgac cttcgccgct
     2581 accct
//
LOCUS       DATGFG010000372         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1280705, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000372 DATGFG010000000
VERSION     DATGFG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280705"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VKQ52_07065"
     CDS             complement(<1..426)
                     /locus_tag="VKQ52_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86979.1"
                     /translation="MRLKSWFPALLLTFFTSALTAQDSASIGLPELMAAEKLFDLRFT
                     PAKRDSILSGLVDHLQFYRYLHTHNLYNDVPLSLSFDPLLPGTAYERRQRPLEWHIPG
                     EVALPADRSELAWYSIPELSSLLRRRKITSVELTKFFIDR"
     gene            478..993
                     /locus_tag="VKQ52_07070"
     CDS             478..993
                     /locus_tag="VKQ52_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLZ86980.1"
                     /translation="MSLEVFKGRVLPMKDKLFRFAFHLLQNIQEAEDAVQDVMAGIWA
                     KRAEWPQWNSIEAYCMTATRNNCLDRLRRRRIHTVQEGSAHQLSSPDHDPHEKMTNKE
                     MIQRIRQCMDALPQQQQQVIRLREMEGFSYNEIADVLDITLDQVKVCLFRGRNAIRKI
                     ITKQEEKWKKQ"
     gene            978..1502
                     /locus_tag="VKQ52_07075"
     CDS             978..1502
                     /locus_tag="VKQ52_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86981.1"
                     /translation="MEKAVILALLERYWRAETTVEEEKTLAAWFSEQAPGSLDPDLEP
                     YHALFAYFDEETQVTPGPGFEARILERVGISEPRPVRQFQWGLVSAAAAVLVLAIGLF
                     LLTPSEPPAGPVSAARIPGTRSPATGTPATPTTATRITDTYDNPDQALAAVRHALLIA
                     SNHLNEGRKQITNK"
     gene            1499..2008
                     /locus_tag="VKQ52_07080"
     CDS             1499..2008
                     /locus_tag="VKQ52_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HLZ86982.1"
                     /translation="MKRILLILTIAITATSAFAQDDAIGRFFGKYIDDPRFTVVSVSP
                     RMFRLMSKINWDTIPSDLKQTVSRLQSFRVLSTETTPGVFFREALTRIDRKEYEELIT
                     VRGNKDNFRFMIRATGNTIHELLMIAAAEDGFTLMSFVGDIDLDKISRLAADMDIKGM
                     ENLKNAKRK"
     gene            2010..2468
                     /locus_tag="VKQ52_07085"
     CDS             2010..2468
                     /locus_tag="VKQ52_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HLZ86983.1"
                     /translation="MKTLTLPLLALLIACAACAQSSHLDKFYQQYNVSDQESPIDPSL
                     LLNVSFSSNKITALHCLIIDGKKTPNAGREFRDLQQSLRADHFEEWFSIRKGEGRIAL
                     LSRDGKDSLEEMACLIVGDDDSGLFFHLRGHFTAADKAKIEAALQQHDSQ"
BASE COUNT          677 a          841 c          663 g          539 t
ORIGIN      
        1 ccgatcgata aagaacttcg tcagctccac gctggtgatc ttccgccgtc gtaacaggct
       61 ggacaattcc gggatggaat accaggcgag ttcactacga tcggccggca gggcaacctc
      121 ccccgggata tgccattcca gcggccgctg cctgcgctcg taggccgtac ccggtaacag
      181 cgggtcaaag gacagggaca gtggtacgtc gttgtatagg ttatgcgtat gcaggtaccg
      241 gtaaaattgg agatggtcga ccagcccgct caggatagaa tcccgcttcg ccggggtaaa
      301 ccgcagatcg aaaagctttt ccgcggccat cagttcgggc aatccaatgg aagcggagtc
      361 ctgcgcggtc agcgcactgg tgaaaaaggt caggaggagg gccggaaacc agcttttaag
      421 acgcatacaa agaatttact gtaacattaa tgtttttctt tcgtcatttc cacagtaatg
      481 tcattagaag ttttcaaagg ccgggttttg ccgatgaaag acaagctgtt ccgctttgct
      541 ttccacctgc ttcaaaacat ccaggaagca gaggacgccg tccaggacgt catggccggc
      601 atatgggcaa aacgggcgga atggccgcaa tggaacagca tcgaggccta ctgtatgacg
      661 gcaacccgga acaattgtct cgaccggctc cggcgccgcc gcatccacac cgtacaggag
      721 ggatcggcgc accagctcag tagtcccgac cacgaccccc acgagaaaat gacgaataag
      781 gaaatgatac aacgcatccg gcaatgcatg gacgccttgc cccaacagca acagcaggtg
      841 atccgcctgc gggaaatgga agggtttagt tataatgaga tcgccgatgt actcgatatc
      901 accctcgatc aggtaaaggt ctgcctgttc agaggccgta acgccatccg gaagatcatc
      961 acaaaacaag aggaaaaatg gaaaaagcag tgatcctggc cctgctggag cgatactggc
     1021 gggcagagac gacggtggaa gaggaaaaaa cgctggcagc ctggttcagc gaacaggctc
     1081 ccggcagcct tgaccccgat ctcgaaccat accacgcgct cttcgcttac ttcgatgagg
     1141 aaacgcaggt aacccccggc cccggcttcg aagcccgcat cctggagcgc gttggtatct
     1201 ccgaacctcg cccggtccgc cagttccaat ggggcctcgt ctccgccgcc gccgcggtcc
     1261 tggtccttgc catcggcctg tttctgttaa caccctccga gcccccggcg ggacccgtct
     1321 ccgcagcccg aatccccgga acacgaagcc ctgcaaccgg aacccccgca acccccacta
     1381 ccgcaacccg catcaccgat acatacgaca atcccgacca ggccctcgcc gccgtccgtc
     1441 atgccctgct catcgcctcc aaccatctga acgaaggccg caaacaaatc acaaacaaat
     1501 gaaaaggatc ctcctcatac tcaccatcgc catcaccgcc acaagcgcct tcgcccagga
     1561 cgacgccatc ggcaggttct tcggcaaata catcgacgac ccacgcttca ccgtcgtctc
     1621 cgtcagcccc cggatgttcc gtctcatgtc gaagatcaat tgggacacga tcccctccga
     1681 tctgaaacag accgtctccc gcctccaaag cttccgcgtc ctcagcacgg agaccacccc
     1741 gggggtattc ttcagggaag ccctgacaag gatcgaccgg aaagaatacg aggagctgat
     1801 caccgttcgc ggcaataaag acaatttccg cttcatgatc cgggctacag gtaacacgat
     1861 ccacgaactc ctgatgatcg ccgccgccga agacggtttc acgctcatga gtttcgtcgg
     1921 cgacatcgac ctcgacaaga tctcccggct cgctgccgac atggatataa aaggaatgga
     1981 aaatttgaaa aatgcaaaac gtaaataaca tgaaaacact caccctcccc ttattggccc
     2041 tcctgatcgc ctgcgccgcc tgcgcccagt cctcgcatct cgacaaattc taccagcagt
     2101 ataacgtcag cgatcaggag tccccgatag acccaagcct cctgctgaac gtcagcttct
     2161 cttcaaacaa gatcacggcc cttcattgcc tcatcatcga cggtaagaaa acgccaaacg
     2221 ccggccgcga attccgcgat ctgcaacaat ccctgcgcgc cgaccacttc gaagaatggt
     2281 tctccatccg caaaggcgag ggtaggatcg ccctcctttc ccgtgacggg aaggacagcc
     2341 tcgaagaaat ggcttgtctc atcgtcggcg acgacgatag cgggctcttt ttccatctcc
     2401 gggggcattt caccgccgcc gacaaagcga agatagaggc cgccctgcag caacacgaca
     2461 gtcaatgacc aaccgcgcgt ccgccgcaca ggtaagtgct tctactttga taataatcaa
     2521 gaattattgt cggtaagtgt ttttacttat tcccagctgg ctgatttcta attaattccc
     2581 attatcttgt cgttaggttc aacaaattgg ttaggtttcc tcacgtagca tagtagcacg
     2641 cctttattct gacctgtctt actctattct ttaacttaac gaacaagaac tatgatcagg
     2701 attctactcg ctgatgattt
//
LOCUS       DATGFG010000373         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1280576, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000373 DATGFG010000000
VERSION     DATGFG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280576"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2122
                     /locus_tag="VKQ52_07090"
     CDS             <1..2122
                     /locus_tag="VKQ52_07090"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HLZ86984.1"
                     /translation="ISNGTAVLGLGDIGPEASKPVMEGKGVLFKIFADIDVFDIEINE
                     KDPEKFVQIVKALEPTFGGINLEDIKAPECFYIETALREKLKIPVMHDDQHGTAIISS
                     AALLNALELQKKKIDKVRFVINGAGAAAMACVKLYVALGARPENFILFDKDGVLHIDR
                     TDLDDEKRKFAVQKNDWTLEKAMKDADVFVGLSVGNVVTPEMVKVMARRPIVFAMANP
                     DPEITYEQATAAREDIIMATGRSDYPNQVNNVLGFPFIFRGALDVRATQINEAMKLAS
                     VKALADLAKTPVPDIVNMAYNEKNILFGPTYIIPKPLDPRLLSTVAPAVARAAMESGV
                     AQHKIDNWEAYAIELNRRLGLDNQLMRVLSSKARLNPKRVVFAEADNLKILKVAQLLY
                     DEGIAFPMLLGDERKIRHLAATNQIDLEGMPVINPYNDEYEEQRKRFGELFFQKRQRR
                     GFNKYEAYKIMRDRLYFGCMMVETGEADAMISGLTKNYPDTIRPALQVIGTEDGVKKI
                     AGMYLLLTKRGPLFLADTTVNFNPTAEELADITLLVAREVQHFNLTPRVAMLSYSNFG
                     TSDSPEARLVAEARQIVKQRDPNLIVDGEVMASVALNNDILRENYPFSELVDQEVNTL
                     VFPNLAAGNIAYNLLKEVGGADAIGPILLGLKKPVHVLQLGSTVRQIYNMALVAIVDA
                     QIKCKNTPSDEEAKKAPWWKRARH"
     gene            2140..2877
                     /locus_tag="VKQ52_07095"
     CDS             2140..2877
                     /locus_tag="VKQ52_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86985.1"
                     /translation="MTFFTEFGRYLLTLRGMFTKPENGKMYWKVFMHQCSEIGIGSLG
                     IVAIISVFMGAVSTLQTAYQLVSPLIPLSTIAQIVRDTVILEFSPTLVCIVLAGVVGS
                     KIASELGNMRVSEQIDALEIMGINTKSYLILPKLLAATAMIPMLVVISMVLGMAGGRL
                     AGTLTGILSPAAFDKGLHQYFKPYNVFFAMAKSYTFAFIISSIPAFYGYNVQGGALEI
                     GRASTKAVVVSCVLVLFADYLLSALLL"
BASE COUNT          719 a          768 c          916 g          635 t
ORIGIN      
        1 gatcagcaac ggcaccgcgg tgctggggct gggcgatatc ggccccgaag cctcgaagcc
       61 ggtgatggaa gggaaggggg tgctttttaa gatcttcgcc gatatcgatg tattcgatat
      121 cgagatcaat gaaaaggacc ccgagaagtt tgtccagatc gtcaaggcat tggagcccac
      181 gtttggcggc atcaacctgg aagacatcaa ggccccggaa tgtttttata tcgaaacggc
      241 cctgcgggag aagctcaaga tcccggtgat gcatgatgac cagcatggga cggccatcat
      301 cagcagcgcc gccctgctga acgcactcga actccagaag aagaagatcg acaaggttcg
      361 atttgtgatc aacggcgcgg gtgcggcggc gatggcctgt gtcaagctct atgtcgcgct
      421 tggcgcccgg ccggagaatt ttatcctttt tgacaaggac ggggtgctgc atatcgaccg
      481 caccgatctc gatgacgaaa aaaggaagtt cgccgttcaa aagaatgact ggaccctcga
      541 gaaggcgatg aaggatgcgg acgtatttgt cggcctcagc gtcggcaatg tggtgacccc
      601 ggaaatggtt aaggtcatgg cccgccggcc gatcgtattc gctatggcga atcctgatcc
      661 ggagatcacc tacgaacagg ctacggccgc gagggaagat atcatcatgg ccacgggccg
      721 gagcgattat cccaatcagg tgaataatgt cctgggattc ccttttatct tccgcggggc
      781 gctggatgtg cgggctaccc agatcaacga agcgatgaag ctggcttccg taaaggccct
      841 ggccgatctc gccaagaccc ctgttccgga tatcgtgaac atggcctata atgagaagaa
      901 catccttttc ggtcctacct atatcatccc caagccactg gacccgcggc ttttgtcgac
      961 agttgcgccg gcagtggccc gggcggccat ggaaagtggt gtcgcgcaac ataagatcga
     1021 caactgggag gcgtatgcca tcgagctgaa ccggcggctg ggtctcgata accagctgat
     1081 gcgggtgctg agcagcaagg cccggctgaa tcccaaacgg gtggtatttg ccgaggccga
     1141 caacctcaag atattaaagg tagcccagct cttgtatgac gaaggcatcg ccttccccat
     1201 gctgctgggt gatgagcgaa agatccgcca tcttgccgca accaaccaga tcgacctgga
     1261 aggcatgccg gtgatcaatc cctataatga cgaatacgag gagcagcgca aacgctttgg
     1321 agaattgttc ttccagaaac ggcagcgccg cggctttaat aaatacgagg cctataagat
     1381 catgcgcgac cggctttatt ttggttgtat gatggtagag acgggcgagg ccgatgcgat
     1441 gatctcgggg ctgacgaaga actacccgga tacgatccgg cctgccctgc aggtcatcgg
     1501 tacggaggat ggggtaaaga agatcgcggg gatgtacctg ctgcttacga agcgggggcc
     1561 gctcttcctt gccgacacca cggtcaactt caacccgacg gccgaggagc ttgccgatat
     1621 cacgctgctg gtggcccggg aggtacagca tttcaacctt acgccaagag tggcgatgct
     1681 gagctattcc aatttcggca ccagcgactc tcccgaggcg cggctggtgg cggaggcgag
     1741 gcagatcgtc aagcaacggg atcccaacct gatcgtggat ggggaggtaa tggcgagtgt
     1801 ggcgctgaac aatgatatcc tccgggagaa ctatcccttt agcgaactgg tggaccagga
     1861 ggtcaatacc ctggtgttcc ccaatctcgc ggcggggaat atcgcgtata acctgttgaa
     1921 ggaggtcggg ggggccgatg ccatcgggcc gatcctgctt gggttgaaga aacccgtcca
     1981 tgtccttcag ctggggagta cggtgcggca gatctataat atggcgctgg tggccatcgt
     2041 cgacgcacag ataaaatgca agaacacgcc ttcggacgaa gaggcgaaga aagcgccctg
     2101 gtggaagcgg gcaaggcact aaaaccaaaa cttttcttaa tgactttttt tacggaattc
     2161 gggagatacc tgctcacctt aagaggaatg tttacgaagc cggagaacgg caagatgtac
     2221 tggaaggtgt tcatgcatca atgttcggag atcggtatcg gctctctggg tatcgtggcg
     2281 atcatttcgg tcttcatggg ggcggtgtcg accctgcaaa ccgcctatca attggtaagc
     2341 cccctgatcc ccctgtcgac gatcgcgcag atcgtgcggg atacggtcat cctggaattc
     2401 tccccgacgc tggtgtgtat cgtcctggcc ggcgtggtag gcagtaagat cgccagtgag
     2461 ctgggcaata tgcgggtgag cgagcagatc gatgcactgg agatcatggg gatcaataca
     2521 aaatcctacc tgatattgcc aaaactgctg gcggctacgg ccatgatccc gatgctggtc
     2581 gtgatctcga tggtgctcgg gatggcagga ggacggctgg ccggcaccct cacgggcatc
     2641 ctttcaccgg cggctttcga caaggggctg caccagtatt tcaaacccta taatgtattc
     2701 ttcgccatgg cgaagtccta tacctttgct ttcatcattt ccagtatccc ggccttttat
     2761 ggctataatg tacagggcgg ggcgctggag atcggcaggg cgagtacaaa ggctgtggtg
     2821 gtgtcctgtg tgctggtatt atttgcagac tatttgcttt cggccctctt gctgtaagaa
     2881 gaagacagca ggaatagcca gccgaaagca aataaaagac attagccccg ttaaggccgc
     2941 cttcggcggc cgtagcggag cgaagagcaa taataagacg atgatcgaaa taaaaaatat
     3001 aaaaaaagga tttggcgaaa agacggtgat cgatgacg
//
LOCUS       DATGFG010000374         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_939134, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000374 DATGFG010000000
VERSION     DATGFG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939134"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VKQ52_07100"
     CDS             complement(<1..712)
                     /locus_tag="VKQ52_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLZ86986.1"
                     /translation="MQELIVGLTTGAIYALIALGYTMVYGIIELINFAHGDIFMIGTL
                     ISITIFGLFGITSSSQPVGLTLVGLLIITCLGSMLVCAVLGVVIERVAYRPLRNSPRL
                     APLISAIGVSLILEDVGKLWKGVTYVSFPQILPQINYYIGPVYISSINILVIVVSIIL
                     MVGLQWMITSTRTGRAMRAVAQDREAAALMGVNVDNIIGTTFFIGAALAGAAGFIYGL
                     QYGSTIFYIGFQLGLIAFT"
     gene            complement(919..2238)
                     /locus_tag="VKQ52_07105"
     CDS             complement(919..2238)
                     /locus_tag="VKQ52_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HLZ86987.1"
                     /translation="MRRSWSRIFALALGLPLLLALLAACGAGTTGGNGGSTATTGSTI
                     IKIATDFPASGKDTSSGKPAQDGANLAVNEANTNHTIPGYTLEFVPKDDVGVSGTHDP
                     AVGAANVRALIGDALVAGIVGPFNSSVAEAEMPITNRAPIAQISPANTNTCLTQQGAA
                     VDCVGATDFVPTLRPTGKVNYFRIAGTDNFQGQAGADYLFKTQHYKSVYVIDDAETYG
                     IGLADSFEKEWKALGATFLGRSSEPGTTTSYVSLLTQIAAKHPDVIYFAGVDSTGGTL
                     VRQQMEQIPSLANLPFGGGDGIVTPAFASTIGLKGGPIFGTIAVKDTVQNPATASFKQ
                     KFLAVYPTINVYSAAAYDCANILIQAIKTALANGAHTPKDSGDAAGAKLFRQAVISAI
                     QNIQYDGVDGHQSFDANGDTNNKVFTIYQLGTVEGKTDWKFISEVVK"
     gene            complement(2333..>2668)
                     /locus_tag="VKQ52_07110"
     CDS             complement(2333..>2668)
                     /locus_tag="VKQ52_07110"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86988.1"
                     /translation="SGGWQLSPDGQYFAFGSWNNSVQVWDTVNGRKIASYPGHIGQVQ
                     VLAWSPDSRRIAIAGTDGTVQVWNVVTGKNIVRYQGLSQTASLTWSLDGKLIAATSEN
                     NTVQVLQAT"
BASE COUNT          631 a          729 c          740 g          568 t
ORIGIN      
        1 ccgtaaaagc aatcaggcca agttgaaagc cgatatagaa aatcgtactg ccatattgta
       61 aaccataaat gaaacccgcc gctcctgcta acgccgcgcc gataaagaag gtggtcccaa
      121 taatattgtc aacgttcacg cccatcaatg ctgctgcttc gcgatcctga gccacagcac
      181 gcatagcacg gcctgtgcgc gtgctcgtaa tcatccactg caggccaacc atcaatataa
      241 ttgaaaccac aatcaccagt atattaatac tgctaatata gacgggtcct atatagtagt
      301 tgatctgggg taaaatttgt ggaaacgaga cataggtaac acccttccag agttttccaa
      361 catcctccag gatcaatgaa acgccaatag ctgagataag tggagcaaga cgtggagaat
      421 tcctcaaggg acgataggcg acgcgttcaa tcactactcc tagtacggcg cacaccagca
      481 tagaacccag acaggtaata atcaagaggc caactaatgt taaccctacg ggctgcgatg
      541 aactggtaat accaaaaaga ccgaaaatag taatcgagat aagggtacca atcatgaaga
      601 tatcgccatg agcaaagttg atcagttcga taataccgta gaccatcgta tatcccagag
      661 caataagcgc gtagattgcc ccggtagtga gcccgacgat cagttcctgc aagagaagtg
      721 tcatagtgtg tttcccacct tcctgccgac tatttacaag cttaaggaga ttatacaacg
      781 ccgaaagatt gaattgaaga gcaaacagag aggtagtcaa caaaggcgaa tatgttgacc
      841 accccctgcg ttatccctgg gctagacaat agcagcatct agcccggata tgtagtgcga
      901 caatactatc agtgacgatc acttgacgac ctcgctgatg aacttccagt cggtcttgcc
      961 ctcgacggtg ccaagctggt agatggtgaa gaccttgtta ttggtatcgc cattggcgtc
     1021 aaaggactgg tgcccatcga ccccatcata ctggatgttc tggatggcgc tgatcaccgc
     1081 ctggcggaag agcttggctc ccgccgcatc ccccgagtcc ttgggcgtgt gcgccccgtt
     1141 ggccagcgcc gtcttgatcg cctggatcag aatattggca cagtcgtagg ccgccgcact
     1201 gtagacattg atggtcgggt agaccgccag gaacttctgc ttgaagctgg ccgtcgccgg
     1261 gttctgcacc gtatccttga ccgcgatcgt cccaaagatc gggcccccct tcaagccaat
     1321 ggtcgaggca aaggctgggg tgacgatgcc atcgccgccg ccaaagggca gattggccaa
     1381 cgaggggatc tgctccatct gctggcgcac caaggtgcct ccggtggagt ccaccccggc
     1441 aaagtagatc acatcgggat gtttggcggc gatctgggtc agcagggaga cataggaggt
     1501 ggtggtaccg ggttcgctac tgcggccaag gaaggtcgct cctaaggctt tccactcctt
     1561 ctcgaagctg tcggccaggc caatgccata ggtctcggca tcatcgatga cgtagacact
     1621 cttgtagtgc tgggtcttaa agaggtagtc ggctccggcc tgtccctgga agttgtcggt
     1681 gccggcaatg cggaagtagt tgaccttgcc ggtgggacgc agggtgggca caaagtcggt
     1741 ggccccgacg cagtcgacgg cagccccctg ctgggtgagg caggtgttgg tgttggctgg
     1801 gctgatctgg gcgatgggag cgcgattggt aatgggcatc tcggcctcgg ccacactact
     1861 gttaaaggga cccacgatgc cagcgaccag ggcatcgccg atcaaggcgc gcacgttggc
     1921 ggccccgaca gccgggtcat gggtgccgct gacgccgaca tcgtctttgg gcacaaattc
     1981 gagggtatag ccagggatgg tgtgattggt attggcttcg ttgacggcca ggttggcgcc
     2041 gtcctgggcg ggtttaccgc tgctggtatc tttaccggat gcgggaaagt ccgtggcgat
     2101 tttgataatg gtggagccgg tggtggcggt cgagccgccg ttgccgccag tggtgccggc
     2161 gccacaggcc gccagcagcg ccagcaagag gggcaggccc agtgctagcg cgaaaatacg
     2221 tgaccaactt ctcctcatga tctagttaac ctccttttgc taaactcgat acgcaacatg
     2281 ctcccccctt tggagagcaa acaacttccg tattgacgat atggcccacc cctcaggtcg
     2341 cttgcaatac ctggacagta ttattctcgc ttgtagcagc aatgagctta ccatccagag
     2401 accacgtcag cgacgctgtt tgggagaggc cctgatacct gacgatgttc ttcccggtta
     2461 ctacattcca cacctgcaca gttccatccg tgccggcaat ggcgatgcgt ctgctgtcag
     2521 gggaccacgc caggacctgg acttgcccga tatgacccgg gtaggaagct atcttgcgtc
     2581 cattgacggt atcccacacc tggacgctgt tattccagct cccaaaagca aaatattgac
     2641 catcaggcga taactgccac ccgccgct
//
LOCUS       DATGFG010000375         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_795291, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000375 DATGFG010000000
VERSION     DATGFG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795291"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VKQ52_07115"
     CDS             complement(<1..1266)
                     /locus_tag="VKQ52_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ86989.1"
                     /translation="MFRNYLKIAFRSLRKSKGYTALNIIGLAAGLGVCLLIVLYVTDE
                     LSYDRYNVNADRIYRIDEDIYLNDTQVDAVTTSKFFGPTLVASYPKIQQMVRFRNPGD
                     QLVRKGNDHVLDHRFTFADSTIFKVFTLPMIAGDPNTALNNPHSIVIDESAARRYFNS
                     TDVIGRTLEIGSNNTPLKITGVIRDMPEQSQFHFSFIRPIREAYSFNDPNDNNWVSNG
                     YYTYILTQPGTTRAEVQKDVDAVVNLNISPALQGMFHTSGADLEKAGNHFRCRIFPLT
                     DVHLHSNKSGELEANSNIQYVYIFSVIAVLILVIACVNFMNLSTARSANRAKEVGIRK
                     VSGSTKGHLILQFLTESILLSLFSLVLALGIAVLLLPMFNQLAGKSLHPDVLFSGRFL
                     PLLILLVLLVGCLAGSYPAFYLSSFQPIDV"
     gene            complement(1491..>2011)
                     /locus_tag="VKQ52_07120"
     CDS             complement(1491..>2011)
                     /locus_tag="VKQ52_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ86990.1"
                     /translation="RFRTNDVFSLVRQVESKFHAAKQGLPFSYSFMDNDFDKLYHAEQ
                     QTGQIFITFAVFAILIACLGLFGLVTYAAEQRTKEIGIRKVLGARVTSIVGLLSKDFT
                     ILVGIAALIAFPAAWWAMYKWLETFAYRTAISWWIFLVAGAVALAIALLTVSIQTIRA
                     ALANPVKSLRSE"
BASE COUNT          454 a          453 c          684 g          420 t
ORIGIN      
        1 tacatcaatc ggctgaaaag aggaaagata gaaggcggga taactgccgg cgagacagcc
       61 gaccagcagc accaggagga tgaggagggg caggaaccgg ccgctgaaga gcacgtcggg
      121 atggagcgat ttgccggcaa gctgattgaa catcggtagc agcagaacgg cgatgccaag
      181 ggcgaggacg agggagaaga ggctgagcag gatggactcg gtgaggaact ggaggatgag
      241 gtgtcctttg gtcgagccgg agaccttgcg gatgcccact tccttggcgc ggttggcgct
      301 gcgggcggtg ctgaggttca tgaaattgac gcacgcgatc acgaggatga gtactgcgat
      361 tacggaaaag atatatacat actgaatatt gctgttggct tcaagctcac cagacttatt
      421 ggaatggagg tgcacgtcgg tgagggggaa gatgcggcac cggaaatgat tacccgcttt
      481 ctcgaggtcg gcgcccgaag tgtggaacat tccctggagg gccgggctga tattgaggtt
      541 gacgactgcg tctacatcct tctggacctc ggctcgcgtg gtgccgggtt gggtaaggat
      601 ataggtataa taaccattgc ttacccagtt gttgtcgttg gggtcattaa aggagtaggc
      661 ttcgcggatg ggccgaatga agctgaaatg gaactgggac tgttccggca tgtcccggat
      721 gaccccggtg atcttgaggg gtgtattgtt actacctatc tccagcgtcc gcccgatgac
      781 gtcggtgctg ttgaaatagc ggcgggcggc actttcatcg atcacgatgg aatgagggtt
      841 gttgagggcg gtattggggt cgccggcgat catgggcagg gtgaagacct tgaagatcgt
      901 cgagtcggcg aaggtgaacc gatggtcgag gacgtggtcg ttgcccttcc tcaccagctg
      961 gtcgcccggg ttgcggaatc ggaccatctg ctggatcttc ggataggagg cgacgagagt
     1021 ggggccgaaa aatttggagg tggtgacagc gtcgacctgg gtgtcattta agtagatatc
     1081 ctcatcgatc cgatagatgc ggtcggcgtt gacattgtag cggtcgtaac tgagctcatc
     1141 ggtgacgtac aggacgatca gcagacagac accgaggcct gcagcgaggc cgatgatgtt
     1201 gagggcggta tagcccttgc ttttcctgag gctgcggaag gcgatcttga gatagttgcg
     1261 gaacatggga tggtcggttt tatgacgagt aaggcggcca aatcatcagg acgatcccaa
     1321 aagagtgccg ggtttataaa atattgaaga ccaatattct gtcaaggcct tggagagacc
     1381 atgatgttcg gtttcgatac agtactcgtg cgggaacgag cattggcggg ggtatgcgag
     1441 acttgccccg ctcctggaat ttccacgcgc ggtcactttg ttaaacattc tcattcgctg
     1501 cggagggatt ttacggggtt agcgagggcc gcgcggatgg tctgaatact caccgtcaac
     1561 agggcaatgg cgagggctac ggcgccggcc acgaggaaga tccaccagct gatcgccgta
     1621 cgataggcaa acgtctcgag ccatttgtac atggcccacc acgcggcggg gaaggcgata
     1681 agcgcagcga tcccgacaag gatcgtgaag tccttgctga gcagaccgac gatgctggtg
     1741 acccgggcgc cgaggacctt gcggatcccg atctccttgg tgcgttgttc ggcagcatag
     1801 gtgacgaggc cgaaaagccc aaggcaggcg atgaggatgg cgaagacggc gaaggtgatg
     1861 aagatctggc cggtctgctg ctcggcgtga tacaatttat caaagtcgtt atccatgaag
     1921 gagtagctga agggcagccc ctgtttggcg gcatggaatt tactttccac ttgccggacg
     1981 aggctgaaga catcgttcgt gcggaagcgg a
//
LOCUS       DATGFG010000376         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_25766, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000376 DATGFG010000000
VERSION     DATGFG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25766"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..808
                     /gene="phoU"
                     /locus_tag="VKQ52_07125"
     CDS             143..808
                     /gene="phoU"
                     /locus_tag="VKQ52_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HLZ86991.1"
                     /translation="MAQIETELQLLKTEAINMWTLVNSQMSKSRQAFVTFDKDLAREV
                     VLKEKRVNGSELKIDRDCENIFALFCPVAIDLRFLLAVLKINSNLERIGDIAEGIAKY
                     IIDSEVTFSEELLKVSRILPMYEESINMLTDTQDAFEKEDTILARSVFKKDDFLDNVN
                     KSSHRVLEEHIKKHPEDVSQALWILSTIRKLERVGDQAKNIAEEIIFYLEAKVLKHKG
                     KFA"
     gene            complement(1068..2105)
                     /locus_tag="VKQ52_07130"
     CDS             complement(1068..2105)
                     /locus_tag="VKQ52_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HLZ86992.1"
                     /translation="MKIFYAVQATGNGHISRAMELLPYFQRYGKVDIFLSGANNSLQL
                     DAAVKYRSRGLSLFYTTSGGLNYWEIAKKVAPMRLWKEVRDLPVEKYDLVINDFESIT
                     SLACMYKKIPSVNFGHQASFISPKVPRPRKQEWMGEWILRNYARATQYVGLHFKQYDD
                     FILPPVIKRDILQAEPTDKGHITVYLSSYNDATVRRYLQPLKEFRFQVFSKEVQRPTL
                     DGNILFLPVSRSAFNKSLISCKAILTGAGFETPAEALYLGKKLMVIPIRGQYEQSCNA
                     AALEKMGVPVLKALDANFASIFRPWMDQRRKPRLHLDYSTESIVSYLMHHCHLEKEGT
                     LDMLYPEAVFN"
     gene            complement(2120..>2420)
                     /locus_tag="VKQ52_07135"
     CDS             complement(2120..>2420)
                     /locus_tag="VKQ52_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86993.1"
                     /translation="DFELIAAELAIEKKYDYVICGHIHQPQKKIVETKDGKVTYLNSG
                     DWVEHCTALEYHQSEWTIFEYNEKDFPVTQTVEMKPSISVLTDEINFYINSLAAI"
BASE COUNT          557 a          569 c          674 g          620 t
ORIGIN      
        1 cttcttcttc ctgggggagc tggtggaata tgacgagaca aagacgatgt tcaccaaccc
       61 caaggaccag cgcacgcaga attatatcac gggtcgcttc agttaattat ctttatacca
      121 ttactatttt catttataac ccatggcaca aattgaaacc gaattacaac tgctgaagac
      181 cgaggccatc aacatgtgga cgcttgtgaa ttcgcagatg agtaaatccc ggcaggcttt
      241 cgtcactttt gacaaggatc ttgcccggga ggtcgtatta aaagagaagc gggtcaacgg
      301 cagtgaattg aagatcgacc gcgactgtga gaacatcttc gccctcttct gtccggtggc
      361 catcgatctg cgtttcctgc tggccgtgct gaagatcaat tccaatcttg agcgcatcgg
      421 cgatatcgcg gaaggcatcg ccaagtatat catcgactcc gaggttacct tctccgagga
      481 gttgctcaag gtgagccgga tcctgccgat gtatgaggag tcgatcaata tgttgacgga
      541 tacgcaggat gcctttgaga aagaggatac catcctggcc cgcagcgttt tcaaaaagga
      601 cgacttcctg gataatgtga ataagagctc ccaccgggtg ctggaggagc atatcaagaa
      661 acatccggaa gacgtgtcac aggcgctttg gatcctgtcc acgatccgca aactggagcg
      721 ggtgggcgat caggcaaaga atattgccga ggagatcatt ttttacctgg aagctaaagt
      781 gctgaagcat aaggggaaat ttgcataggg tctaatcata atattacgat aactattcag
      841 taaactcccc gtaatacggg gagtttactt ttgatagagc aaatgtttat ttggttgtac
      901 gcctaccgct tagtcacctg ttaatcgatg gtttatatat gtagagttga attttctcat
      961 gcagaagcca cttcatttct atgatggtgg cttttttgtt tgatcgctgc tccgctgcgc
     1021 cgtcgcttgc ggcggcgcgg cccgacaagc aagtccggca atctgatcta gttgaagacc
     1081 gcctccgggt acagcatatc cagcgtaccc tccttctcga ggtggcaatg atgcattaaa
     1141 tagcttacga tcgactcggt ggagtagtcg aggtggagac ggggtttccg ccgttgatcc
     1201 atccaggggc ggaagatgga tgcaaagttg gcatccaggg ccttcaggac ggggacgccc
     1261 atcttttcga gggccgcggc attgcaggac tgctcgtact ggccgcggat ggggatgacc
     1321 atcagtttct tacccaggta gagggcttcg gcgggggtct cgaagccggc gcctgtgagg
     1381 atggccttgc aggagataag gcttttgttg aaagcgctcc tgctgacggg caggaagagg
     1441 atattgccgt cgagggtggg gcgctgcact tctttggaga atacctggaa acggaactct
     1501 tttaagggct ggagatagcg gcgaacggtg gcgtcgttat aggaagagag ataaacggtg
     1561 atatgtccct tatccgtcgg ctcggcctgc aggatatccc gtttgatcac gggcggcagg
     1621 ataaaatcgt cgtattgttt gaagtggagc ccgacatact gggtagcccg ggcgtagttg
     1681 cggaggatcc attcccccat ccattcctgt ttccggggcc ggggaacctt gggcgagata
     1741 aaacttgcct ggtggccaaa attgacggag ggtattttct tgtacatgca ggccagggag
     1801 gtgatggatt cgaagtcatt gatcaccaga tcgtatttct ctaccggcag atcgcgtacc
     1861 tctttccaca gccgcatggg cgccaccttt ttggcgatct cccagtaatt gaggccgccg
     1921 ctggtggtat agaaaaggct caggcccctg cttctgtatt ttaccgcggc atccagctga
     1981 aggctattgt tcgcaccgct caggaaaatg tctacttttc cgtagcgttg aaagtagggg
     2041 agtaattcca tcgcccggct gatatggccg ttgcccgttg cctgtactgc gtaaaaaatc
     2101 ttcataagcg gtgatgcggt tatatagctg caagagagtt gatgtaaaaa ttgatctcat
     2161 ccgtgagtac gctgatggat ggcttcatct cgacggtctg cgttaccggg aagtcctttt
     2221 cgttatactc gaagatggtc cattcgctct gatggtactc cagtgccgtg caatgctcga
     2281 cccagtctcc ggaattcaga taggtgacct tcccgtcctt cgtttccact atcttcttct
     2341 gcggctgatg gatatggccg cagataacat aatcatactt tttttcaatg gcaagttcgg
     2401 cggcgatgag ttcgaagtcg
//
LOCUS       DATGFG010000377         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1031496, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000377 DATGFG010000000
VERSION     DATGFG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031496"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VKQ52_07140"
     CDS             complement(<1..482)
                     /locus_tag="VKQ52_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86994.1"
                     /translation="MSRFLSSLLLTLTLMTWSAAKAQVQQITYSEPEREDGRRTNFEI
                     IGKLSGNYLVFKNNGSTNAISIYDSAMKLQQRVPLGFMPDRYINVDFVAYPEFFYLIY
                     EYQRKGIVHCTAVKLNAQAQKMGEPVELDTTQVSFAANNKIYTTVISEDKQRIMVFKI
                     "
     gene            722..>2626
                     /locus_tag="VKQ52_07145"
     CDS             722..>2626
                     /locus_tag="VKQ52_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HLZ86995.1"
                     /translation="MSKQINKVSFANLVIALGIIYGDIGTSPLYVLNAIIHGRIIEEK
                     LILGALSCIIWTLTLQTTVKYVWLTLKADNKGEGGIFSLYALVRRQKRWLVVPAMLGG
                     AALLADGMITPPISVTSAIEGLRQMPAFRYVDQSTIVYLVIGILVILFFLQQFGTESI
                     GKLFGPIMFIWFSMLAVLGCNHLLDDLGIFRALSPHYAIQLLTTYPKGFWILGAVFLC
                     TTGAEALYSDLGHCGRGNIRYSWIYVKACLILNYLGQGAWLMAMYKGRVFDTNLGNPF
                     YNIMPPWFVIPGIIIATAAAIIASQALITGSFTLISESMRLNLWPKLRISYPTEERGQ
                     SYIPAINFLLFLGCTGIVLYFRSSSHMEAAYGLAITLCMLATSILFANYMISRRTNSL
                     LIYIYLVVYLSIELSFLFANLEKFPHGGFVTLIVGGGLFTVMFVWFRARKIKNRYVEF
                     VRMEHYIPQIQELSNDRTVPKSATHLVYMTSANNPKEIEHKIIYSILNKKPKRADIYW
                     FVHVDTLDDPYTAEYSVEHIIPNDIIRVEFRLGFRIEPKINLMFRKVVEDLVNNREVN
                     ITSRYESLERNNVVGDFQFIVMEKYLSQDNELPFWERVVMKLYFWLKELSLSEERGFG
                     LDPSNVTIEKF"
BASE COUNT          573 a          723 c          688 g          642 t
ORIGIN      
        1 ttgatcttaa agaccatgat ccgctgtttg tcctcgctga tgaccgtggt atagatctta
       61 ttgttggcgg caaagctcac ctgggtggtg tccagctcca ccggctcacc catcttctgc
      121 gcctgggcgt tcagcttcac cgccgtgcag tggacgatgc ctttgcgctg gtattcgtag
      181 ataagataga agaattccgg gtaggctaca aagtcgacgt tgatgtacct gtccggcatg
      241 aagccaaggg gcacccgctg ctgcaatttc atcgcgctgt cgtagatgct gatggcgttg
      301 gtggagccgt tattcttgaa taccaaatag ttaccactca atttgccaat gatctcgaaa
      361 ttggtacgac ggccatcctc cctctccggt tcgctatagg tgatctgttg aacctgggcc
      421 tttgcagcgg accaggtcat cagggtaagt gtgagaagca gtgatgacag gaatctgctc
      481 atatcgattt aattataagc gcgaataggt ctttaacctg aaaattagcc ctattcgcgc
      541 aaacggctaa tgtctgacct tcggtcgaac cgcttccagc ggttcctgtt acggttgtaa
      601 gataggtaaa agtttgactg ttattaccta cggtttcttt aaaagttggg ttgccgggat
      661 gggcggcgga gcctaaaatt agcttgtacg tttcatgctt ttcgccctaa ctttgccgac
      721 tgtgagtaaa cagatcaaca aagtaagttt tgctaacctt gttatagcat taggcattat
      781 ctacggcgac atcggaacct cccccctcta cgttttaaac gccatcatcc acggaaggat
      841 aatcgaagaa aagctcatcc tgggcgccct gtcctgtatc atctggacac tgaccctgca
      901 gaccaccgta aaatatgtct ggctcacctt aaaggctgat aataaaggag aaggggggat
      961 cttctccctc tatgccctgg tcaggcggca aaagcgctgg ctggtggtcc ccgccatgct
     1021 gggaggtgcg gcgctgctgg ccgacggcat gatcaccccc ccgatatccg ttacttccgc
     1081 tatcgaaggg ttgcggcaga tgccggcctt ccggtatgtg gaccagtcca cgatcgtcta
     1141 cctggtgatc ggtatcctgg tgatcctttt ctttctgcag cagttcggga cggagtcgat
     1201 cgggaagctc ttcggaccga tcatgttcat ctggttttcc atgctggcgg tactggggtg
     1261 caaccacctg ctcgacgatc tgggtatctt cagggccctt agcccgcact atgccatcca
     1321 gctgctgacc acctacccga agggtttctg gatcctggga gccgttttcc tttgtaccac
     1381 gggggccgaa gccctctatt ccgacctcgg gcactgcggc cgcgggaata tccgctactc
     1441 ctggatctat gtaaaagcct gcctgatcct gaactatctc ggccagggcg cctggctgat
     1501 ggcgatgtac aagggcaggg tcttcgacac caacctggga aaccccttct acaatatcat
     1561 gcccccctgg ttcgtcatcc cggggatcat catcgcgacg gcggcggcga tcatcgccag
     1621 ccaggcgctg ataacaggct ccttcaccct gatcagcgag agcatgcgcc tgaacctctg
     1681 gcccaagctg cggatcagct atccgacgga agagcgcggg cagtcctata tcccggccat
     1741 caatttttta ctgttcctcg gttgcacggg gatcgtcctc tacttccgga gctcctccca
     1801 tatggaagcg gcctatggcc tggccatcac gctttgtatg ctggcgacct ccatcctttt
     1861 tgccaactac atgatctccc ggcggaccaa ttccctgctg atctatatct acctcgtggt
     1921 ttacctcagc atcgaactca gcttcctgtt cgccaacctg gagaaattcc cgcatggcgg
     1981 ctttgtcacc ctgatcgtcg gcggcgggct gttcaccgtg atgttcgtct ggttccgcgc
     2041 ccgtaagatc aagaacaggt acgtggagtt cgtgcggatg gagcattata tcccgcagat
     2101 ccaggagctg agcaatgacc ggacagtacc caagtcggcc acccacctgg tgtatatgac
     2161 cagcgccaac aacccgaagg aaatagaaca taagatcatc tattccatcc tgaacaagaa
     2221 acccaaacgg gcggatatct actggttcgt ccacgtggat accctcgacg acccgtacac
     2281 cgccgaatat tcggtggaac atatcatccc caacgatatc atccgggtag agttccggct
     2341 cgggttccgg atcgagccca agatcaacct gatgttccgg aaagtggtgg aagacctcgt
     2401 caacaaccgg gaagtgaata tcaccagccg gtatgaatcc ctcgaacgga acaatgtggt
     2461 cggagatttc cagtttatcg tgatggaaaa atacctgagc caggacaatg agctgccttt
     2521 ttgggagagg gtggtgatga agctgtattt ctggctgaag gagctgagct tatccgaaga
     2581 gcggggattt ggcctggacc cgtccaatgt gacgatcgag aaattc
//
LOCUS       DATGFG010000378         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_401918, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000378 DATGFG010000000
VERSION     DATGFG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401918"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1073
                     /locus_tag="VKQ52_07150"
     CDS             105..1073
                     /locus_tag="VKQ52_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HLZ86996.1"
                     /translation="MRTNYTLIIIGGGPIGIACALEAQKAGLDYLILERGCLVNSLYR
                     YPANMTFFSTSERIEIGGVPFVSNNVKPNRSEALEYYRRVAVSNAVQIRLQEGVSEIR
                     PVADGFEVISERGVYIAAHVILATGFYDIPVLLDVPGEELPKVTHYYREPHFYAMQKV
                     IVVGASNSAVDAALETWRKGAEVSMVIRGEGIGPRVKYWVKPDIENRIKEGSIKTYTH
                     SVLTAIREDEVDILTPEGLTTIPNDYVIALTGYQPDFNFLRRTGIRLSDDGRLQPYYD
                     PETMETNIPGLYLAGVVCGGMNTHVWFIENSRIHAEMIIRNIVNKG"
     gene            complement(1070..2317)
                     /gene="pepT"
                     /locus_tag="VKQ52_07155"
     CDS             complement(1070..2317)
                     /gene="pepT"
                     /locus_tag="VKQ52_07155"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HLZ86997.1"
                     /translation="MFENYNFTVAERFLRYVRIDTQSDPRSTSHPSTEKQKNLGRLLA
                     EELRATGLVDAALDDFGYVYATLPANTDKAVPVICFCAHMDTAPDCTGENVRPVVHPN
                     YDGGDIILPDDPAQVISPREHPYLATRKGDDIITASGTTLLGADDKAGVAVIMDMVHF
                     LTQNPQLRHGAIRILFTPDEEIGRGVDKLDMARLGADFGYTLDAGERGSFEDETFSAD
                     AMTVTFHGISAHPGYGKDKLVNAIKAAAHFVDSLPKDEWSPETTDDRYGFVHPVRISG
                     IAEKATVEFIIRDFITGKLKAYEAHLQDKIEDTLKAFPGATAEVAVTEQYRNMKEVLD
                     QHPRVTGYALEAIERAGIAVHRSSARGGTDGSRLSFMGLPCPNIFTGEMAFHGKHEYV
                     SIQDMQAAVRSIVHLTLIWEERS"
     gene            2349..3263
                     /locus_tag="VKQ52_07160"
     CDS             2349..3263
                     /locus_tag="VKQ52_07160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HLZ86998.1"
                     /translation="MSDQAIKNPVLRQGWLRVALFCVSFCVLTLLITIPAVVLVAGVT
                     TDQLKEQLIPTLSGLLTSNYLWLMVLLEFAVAMICVGIFRLWIDRRSLGSLGWSLDAF
                     PGEAVTGFLMGPALLGISALLMMASGHLEWTDITFDPSSLFVSLGLMALIAFSEEMVF
                     RGYILGNLLDSFGNKWVALILSGVLFAAFHFTNPGIHTLAFVNLFLAGLLLGVNYIYT
                     RNLWFSFLFHLSWNYFQGPILGFRVSGLSLPSLLVAEPKGDLFITGGDFGLEGSILNT
                     TVSVIALCVLALVFEKKYSQAGGIMSKA"
     gene            complement(3253..>3492)
                     /locus_tag="VKQ52_07165"
     CDS             complement(3253..>3492)
                     /locus_tag="VKQ52_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ86999.1"
                     /translation="RDPEAIIEFPIDQFPPQMKPEVGMQLVMTDNSGNQIPVLVLEVK
                     PDAVILDANHQLAGQDLIFDLELVEINSPSRIIMP"
BASE COUNT          664 a          902 c         1063 g          863 t
ORIGIN      
        1 cgacgaggac cacgataatt gtgaggatat tgcccgactc catatctaaa gatttgtggg
       61 tgaatgtaca taaatttgtg cgcgaagcgg cttattttac aaaaatgcgt acgaattaca
      121 ccctcatcat tatcggcggc ggtcctatcg gtattgcctg cgcactggaa gctcaaaagg
      181 cggggctcga ctatcttatc ctggagagag gctgtcttgt caattccctt taccggtatc
      241 cggccaatat gaccttcttc tccacgtcgg agcggatcga gatcggcggt gtcccgtttg
      301 tcagcaataa tgtcaaaccc aaccgcagcg aggcgctgga atattatcgc cgggtggcgg
      361 tgtcgaatgc cgtgcagatc cggctgcagg aaggggtaag tgagatccgc ccggtggctg
      421 atgggttcga agtgataagt gagcggggag tgtatattgc cgcgcatgtc atcctggcta
      481 cgggctttta cgatatcccg gtgttgctcg atgttcccgg cgaggagctg cccaaggtga
      541 cgcactatta ccgtgaaccg catttttacg ccatgcagaa ggtgatcgtg gtgggggcca
      601 gcaattcggc cgtggatgcg gccctggaaa cctggcgcaa gggggccgag gtatcgatgg
      661 tgatccgcgg cgagggtatc ggcccgcggg tgaagtattg ggtgaagccc gatatcgaga
      721 accggataaa ggaagggagc atcaagacat acacgcattc ggtgctgacg gccatccgcg
      781 aggatgaggt ggatatcctt acgccggaag ggctaacgac tattcccaat gattatgtca
      841 tcgccctgac gggttatcaa cccgatttca attttctgcg ccgtacgggg atccggttgt
      901 cggacgatgg ccggttgcag ccttattacg acccggagac catggaaacg aatattcccg
      961 gtctatacct tgcgggtgtc gtttgcggcg ggatgaacac gcatgtgtgg tttatcgaga
     1021 actcgcggat acacgcggag atgatcatcc gaaatatcgt caacaagggt taggatcgtt
     1081 cttcccagat gagggtgaga tggacgatgc tgcgtacggc ggcctgcatg tcctggatgc
     1141 tgacatattc gtgtttgccg tggaaggcca tttcccccgt aaagatattg ggacagggaa
     1201 gacccataaa ggaaagccgg gatccatcgg tgccgccgcg ggcgctgctg cgatggacgg
     1261 ctatgccggc tctttcgatc gcttccagtg catatccggt gacccgggga tgctggtcca
     1321 gcacctcctt catatttcgg tattgttcag tgacggcgac ttcggcggtg gcgccgggga
     1381 aggccttcag ggtatcttct atcttatcct gcaggtgggc ttcataggct tttagttttc
     1441 ccgtaataaa atcgcggatg atgaattcca ccgtcgcctt ttcggcgatg ccggagatgc
     1501 ggacggggtg gacgaagccg taacggtcat ccgtcgtttc gggtgaccat tcgtcctttg
     1561 gcaggctgtc cacaaaatgt gcggctgcct ttatcgcgtt gaccagtttg tccttcccat
     1621 accccgggtg ggcgctgatg ccgtgaaagg taacggtcat agcatcggcg gagaaggttt
     1681 cgtcctcgaa ggaaccgcgt tcgcctgcat cgagggtata cccgaaatcg gcgcccaggc
     1741 gggccatatc caatttgtcc actcccctgc cgatctcttc gtcgggggta aagaggatgc
     1801 ggatggcgcc gtgccggagc tgtgggtttt gggtcaggaa atggaccata tccatgatca
     1861 cggcgacgcc ggccttatcg tcggcgccca gcagggtcgt gccggaggcg gtgatgatat
     1921 cgtcgccttt tctcgtggcc aggtagggat gttcccgggg gctgatgacc tgggcagggt
     1981 catccggcag gatgatgtcg ccgccgtcgt agttagggtg gaccaccggc ctgacgttct
     2041 cgccggtgca gtcgggggcg gtatccatat gggcgcagaa acagatgacc ggtaccgctt
     2101 tatccgtatt ggccggaagg gtggcataga catatccaaa atcatcgagg gcggcatcga
     2161 caaggccggt ggccctgagc tcttcggcca ggagacggcc caggttcttc tgcttttccg
     2221 tcgatggatg gcttgtcgac cgggggtcgc tttgggtatc gatccggaca tagcggagaa
     2281 atctttccgc cacagtaaag ttatagttct cgaacatctt tgtaaattac caggcgaata
     2341 taacacgtat gtccgatcag gctataaaaa atccggtgtt gcgtcagggc tggttgcggg
     2401 tagcgctttt ttgcgtcagc ttttgcgtgc tcaccctgct tatcaccatc cccgcggtgg
     2461 tcctcgttgc cggggtcacc accgatcaat taaaagagca gttgatcccg accttgtcgg
     2521 ggctgctgac gagcaattat ctctggctga tggtcctgct ggaatttgcg gtcgcgatga
     2581 tttgtgtggg cattttccgg ctctggatcg atcgccgcag tcttgggagt cttggctggt
     2641 cgctggatgc ctttccgggc gaggccgtca ccgggtttct tatgggaccg gcgctgctcg
     2701 ggatctccgc cctgctgatg atggccagtg gtcatctcga atggaccgat atcacgttcg
     2761 atccttcctc gctgttcgtc tccctcgggc tgatggcgct gatcgccttc agtgaggaga
     2821 tggtattccg gggatatatc ctgggcaacc tgctggattc cttcgggaac aagtgggtcg
     2881 cgctgatcct ttccggagtg cttttcgcgg cttttcattt caccaacccg ggcatccata
     2941 cgcttgcctt tgtcaatctt ttcctggcgg ggctgctgct cggcgtcaat tatatctaca
     3001 cccggaatct ctggttctct tttctgttcc acctgagctg gaactatttc cagggcccca
     3061 tccttgggtt ccgggtaagc gggctcagcc tgccttccct gctggtggcg gagccaaaag
     3121 gcgatctttt tattacgggg ggagatttcg ggctcgaagg ttcgatcctg aatacgaccg
     3181 tatcggttat cgcgctttgc gtcctggccc tggtctttga gaaaaagtat tcccaggccg
     3241 gcggaataat gtctaaggca tgatgatccg ggaagggctg ttgatctcga ccagttcgag
     3301 gtcgaagatc aggtcctggc ctgccagctg gtgattggca tccagtatca ccgcatcggg
     3361 ctttacctcc agtaccaaca ccgggatctg atttcccgaa ttatctgtca taacgagttg
     3421 catgcccact tcgggcttca tctgcggcgg gaactgatcg atcgggaatt caatgatcgc
     3481 ttcggggtcc cg
//
LOCUS       DATGFG010000379         4013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_140892, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000379 DATGFG010000000
VERSION     DATGFG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140892"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VKQ52_07170"
     CDS             complement(<1..833)
                     /locus_tag="VKQ52_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HLZ87000.1"
                     /translation="MLKSMTGFGRAEQAVGDKTFQVDIKSLNGKQFELQLKLPGFLKP
                     FEFSIRKLISEKLGRGSLDCTISLKQTGNAKPVSINTDLARAYYRALADLSNELQLDP
                     SNILSTLVKLPEVITPTSDTLTDEEWKEFEKVILSAIEDLDAHRLIEGASLEEDLLLR
                     ITNIVRHQDEVVKLEPLRQQKIRDGLKKLLEEHVGKENYDPNRLEQELIYYIEKIDIS
                     EEQVRLKNHCDYFRVILQEQEDSKGKKLSFILQEIGREINTTGSKAYDSTIQQCVVLM
                     K"
     gene            complement(881..1489)
                     /locus_tag="VKQ52_07175"
     CDS             complement(881..1489)
                     /locus_tag="VKQ52_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLZ87001.1"
                     /translation="MLANARQYYVVYKPFQVLSQFTTGEGKATLKDFFPVPADCYPVG
                     RLDFDSEGLLILTNDASLNDRLLNPRFAHDREYWVQVEGAITPAAIHQLETGVTITVD
                     GKPYHTRPAVVATFETPPMVPDRHPPIRFRAQIPTTWIRMVLREGKNRQVRKMTAQTG
                     FPTLRLIRYRIERVNLQGLAPGEMRSITRQQLYSLLFAERNQ"
     gene            1714..2655
                     /locus_tag="VKQ52_07180"
     CDS             1714..2655
                     /locus_tag="VKQ52_07180"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HLZ87002.1"
                     /translation="MQFPSPVSIQWLAEFLGAELIGDKNGQATGINEIHKVEKGDLVF
                     VDHPKYYDTCLRSAASFIIINKSVEVPPGKALLVVDQPFDAYLKIVRHFRPFEPAKKM
                     ISDSAVIGKDTYLYPGVFVGHHTTIGNNCVIHPNVTILDHCVIGDNVIIQAGTVIGSD
                     AFYYNGKKDREVWYRKMESCGRVVIEDFVEIGAGCTIDRGVSHDTVIGRGTKMDNMIH
                     VGHDTVTGKNCLFAAQVGIAGAVKIGNGVILWGQVGVSKTLSIGDNAVVMAQSGVPAS
                     LEGGKSYFGYPAEDAALKKRELVWIKRIPELWKKVMG"
     gene            complement(2772..3851)
                     /locus_tag="VKQ52_07185"
     CDS             complement(2772..3851)
                     /locus_tag="VKQ52_07185"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ87003.1"
                     /translation="MKRKRPLLLLLCTFLFSLAVLSQEKTFPIDGVVLDKAGQPLVGA
                     SVFCQNTTVGTLSKNDGGFHLRLANGGYDLVISYTGYETQVIRIGKDHKPTDTLTIQL
                     KEQDKSLEQAVVTGSAEVADGWAKYGQFFLDNFIGTTPNAAQCTLDNKDVLKFYFYKK
                     RNKLRIKATDLLIVTNNALGYRIKYQLDSFVYDYTGNIGSYTGYPLFEALDGSPDQQQ
                     TWKQNRLYSYSGSRLHFIRSWYDSTLTEEGFVLELADSAGHMKAIGNPYDPAFYSRDS
                     GDLEIGIQGRLRVSYTSQAPDKKYLVQNKYPLTTAAQISALDIVNGFVIEENGYFYDQ
                     GDVSNIGYWSWKKLAELLPYDYLPE"
BASE COUNT          858 a          929 c         1184 g         1042 t
ORIGIN      
        1 tccttcatca atacgacgca ttgttggatc gtggaatcgt aggcttttga gccggtggtg
       61 ttgatctccc ggccgatctc ctggaggata aaggacagtt ttttcccctt gctgtcttcc
      121 tgttcctgga ggatgacccg gaaatagtcg cagtggttct ttagccggac ctgttcttcg
      181 ctgatatcga tcttttcgat gtagtagatc agttcctgtt ccagccggtt ggggtcgtag
      241 ttctctttgc cgacatgttc ttccagcagc tttttcagac cgtccctgat cttttgctga
      301 cggaggggtt ccagtttgac gacctcgtcc tggtggcgta cgatattggt gatccgcagc
      361 agcaggtctt cttccaggga ggcaccttcg atgagccggt gggcgtcgag gtcttcgatg
      421 gcggaaagga tgaccttttc gaattccttc cattcttcgt cggtgagcgt atcgctggtt
      481 ggggtgatca cttccggcag tttgacgagg gtgctcagga tattggaggg gtcgagttgt
      541 aattcgttgg agagatcggc gagggcgcga taataggccc gggcgagatc ggtgttgatg
      601 ctgaccggtt tggcgttacc agtttgtttc aggctgatgg tgcagtccag tgaaccccgg
      661 cccagttttt cggaaatgag tttccggatg gagaattcga agggttttaa gaagccgggc
      721 agtttcaatt gcagttcaaa ttgttttccg ttgagggatt tgatgtccac ctggaaggtc
      781 ttgtccccta ctgcctgttc tgcacgaccg aaacctgtca tcgatttgag cataatatca
      841 atgttttagt gagccttttg cagctcgtta tggtcccaag ttactgattc ctttcggcaa
      901 acagcaagga atagagttgt tgccgggtga tggatcgcat ttccccgggc gccagtccct
      961 gcaggttgac ccgttctatc cggtaacgga tcagtcgcaa tgtcggaaag ccggtttgtg
     1021 cggtcatctt ccgtacctgt ctgttctttc cttcccggag caccatcctt atccatgtcg
     1081 tcgggatctg agcgcgaaag cggatgggtg gatgccggtc gggtaccatt ggcggcgttt
     1141 cgaaggtagc gacaacggcg ggccgggtgt gatagggttt gccgtcgacg gtaatggtca
     1201 cgccggtctc caactgatgg atggcggcgg gggtgatggc cccttccacc tgtacccagt
     1261 attcccggtc gtgtgcgaac cgggggttca gtaaccggtc gttcagggag gcgtcattgg
     1321 tcaggatcag gagcccttcg ctgtcgaaat ccaggcggcc gacgggatag caatccgcgg
     1381 gaacgggaaa gaaatctttt aatgttgcct ttccttcccc ggtggtgaac tgtgagagca
     1441 cctggaacgg tttgtataca acgtagtatt gacgggcatt tgcgagcatc gatctctttt
     1501 ggcgagtggg ttttacaaaa gaaaaagccc ggtgcgaaac cgggctttca atatatggat
     1561 gggttagtaa gtaccgggaa ataaatttcc ctacacaata ttaaaaataa ttttgtctat
     1621 ctaaaaaaat tttccaacaa attttattca catttttttt taggctgtgg aaaatggtcg
     1681 tgcttcaacg ctaccttcac acaaattttg actatgcaat ttccttctcc cgtatccatt
     1741 caatggctgg cggagttcct gggcgccgag ttaattggtg ataaaaacgg ccaggccacc
     1801 ggtatcaacg agatccacaa agtagagaag ggtgatcttg tctttgtcga tcatcccaaa
     1861 tactatgata cctgtctccg tagcgccgcg tcgttcatca tcatcaataa gtccgtggaa
     1921 gttccgccgg gcaaggcttt actggtagtc gaccagccct tcgatgccta tctgaagatc
     1981 gtccggcact tccgtccttt tgaaccggcg aaaaagatga tcagtgattc cgccgtcatc
     2041 gggaaggaca cctatctcta tcccggcgtt tttgtgggtc atcacacgac gatcgggaat
     2101 aactgtgtca tccatcccaa cgtgaccatc ctggatcact gcgtcatcgg ggacaatgtg
     2161 atcatccagg ccggtacggt catcgggtcg gatgcttttt actataacgg caaaaaggat
     2221 cgcgaggtat ggtaccggaa gatggaaagc tgcggccggg tggtgatcga agactttgtg
     2281 gagatcgggg caggctgtac catcgaccgg ggggtaagtc atgataccgt tatcggcagg
     2341 gggacgaaga tggataatat gatccatgtc gggcatgata cagtcaccgg caaaaattgc
     2401 cttttcgccg cccaggtcgg cattgcgggt gcagtgaaga tcggcaatgg ggtcatcctg
     2461 tggggacagg tgggtgtgag caagacgctt agcatcggtg ataatgccgt cgtaatggcg
     2521 cagagcgggg tgcctgcttc cctcgagggg ggtaagtcct attttggcta tccggccgag
     2581 gatgccgcgc tgaagaagcg ggaattggtt tggataaagc ggatccccga actgtggaag
     2641 aaggtgatgg gttagccgtc cgcggggaag gcaggtgtgt taatccggga gacaggggaa
     2701 ggcaggcgtg ttattgcgga agacaggaga aggcagatgt gttattccga agattgggga
     2761 agccaggtct gttattccgg aagataatca tacggcagca gctcggccag tttcttccat
     2821 gaccagtagc cgatattgct tacatccccc tgatcgtaga aatacccatt ctcctcgatg
     2881 acgaagccgt tgacgatatc cagggcggag atctgggccg ccgtggtgag cgggtatttg
     2941 ttctgaacga gatatttctt atccggggcc tggctggtat agctaaccct tagtctgccc
     3001 tggataccga tctccaggtc gccgctatcc cgcgaataga aggccggatc gtagggattc
     3061 ccgatcgcct tcatatggcc ggcgctatcg gccagttcga ggacgaaccc ttcctccgtg
     3121 agtgtgctgt cgtaccagct gcggataaaa tgcaagcgcg agccggaata ggaatagagc
     3181 cggttctgct tccaggtctg ctgctggtcg ggggagccgt cgagggcttc gaataaggga
     3241 tatcctgtat agctgccgat attgccggtg tagtcgtaaa caaaagaatc caattgatac
     3301 tttatccgat agccaagggc attattggtg acgatcagca gatcggtggc ctttatccgg
     3361 agcttattgc gcttcttata gaaatagaat ttaagaacgt ccttgttgtc gagggtgcat
     3421 tgggctgcgt ttggggtggt gccgataaaa ttgtcgagga agaactgacc gtatttcgcc
     3481 cagccgtcgg cgacttcggc gctaccggta accactgcct gctcgaggct cttgtcctgt
     3541 tccttcagct ggatggttaa cgtatcggtg ggtttatggt ctttgccgat gcggatgacc
     3601 tgtgtttcgt agccggtgta ggaaatgacc aggtcgtagc ccccattggc gaggcggagg
     3661 tggaagccgc cgtcattctt cgaaagtgtc cctaccgtcg tattctggca gaagaccgaa
     3721 gcgccgacaa gcggctgacc ggctttatcg aggaccacac cgtcgatggg aaaggtcttt
     3781 tcctgggaaa gaacggcgag ggagaacaga aaggtacaga ggagcaggag taagggcctt
     3841 ttccttttca tggatatcgt tttgtggcgt taagttaact aattatccgc agatagtcca
     3901 gacaggcttc ccgtatcgtc ttttcgagtg gagtgaattg aaaatcgggc aattgtgcaa
     3961 ggatcttgct gttatcgaaa aaggtaaaac tctgtgcgac ccgcgcgctt tcc
//
LOCUS       DATGFG010000380         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1169310, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000380 DATGFG010000000
VERSION     DATGFG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169310"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..848)
                     /locus_tag="VKQ52_07190"
     CDS             complement(159..848)
                     /locus_tag="VKQ52_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ87004.1"
                     /translation="MAEIIVELKHANIYQSANLILQDVNLSVDKGQFVYLVGKTGTGK
                     SSLLKTLYGDLPLREGEGQVAGFDLREMDWKKVPYLRRTLGVVFQDFQLLTDRSVNDN
                     LKFVLRATGWKDMRLMDEKVADVLDKVGLKAKGFKMPFEMSGGEQQRVDIARALLNSP
                     KLILADEPTGNLDPETSDEIMQLLFQISRDYGTTIIMATHDYRVINKFPARMVRTERG
                     QVIDNASLSIE"
     gene            complement(983..2287)
                     /locus_tag="VKQ52_07195"
     CDS             complement(983..2287)
                     /locus_tag="VKQ52_07195"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLZ87005.1"
                     /translation="MSPENSKRQRVVIVGAGFAGLRIARSLNNTAYDVFLIDRHNYHQ
                     FQPLMYQVATARLEPSSISFPLRKVFQHTHNVTIRIADVLRVDVVNKMLETTIGDITY
                     DRLVLCIGCTTNYFGNERIRANSFPMKSVPNAMALRNRILQTFEDTIEAGPDERQSLL
                     NFVIVGGGPTGVELAGAMAEMKKNILPKDYPEVDFSRLTIYLIEGSPNTLNSMSEVSR
                     ATSRKYLEQLGVMVRTGTTVEDYDGNTVRLKGGETIVARNLIWAAGVTGNVLEGIPAS
                     VMVRGNRLKTNRFGEVEGLDGVYAIGDIAYMPTPKYPNGHPQLANVAIAQAAHIAINF
                     KRELKGRTRIEFEYHNKGTMATVGKRKAVVDLPGLSFQGVFAWLTWMFVHLMLLLNVK
                     NRIIVFLNWMMAYFANDSTLRLMLRADRGEGAPEKIERPVMK"
     gene            2491..3063
                     /locus_tag="VKQ52_07200"
     CDS             2491..3063
                     /locus_tag="VKQ52_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLZ87006.1"
                     /translation="MEYPLSQLQLHHPLSGEFIHTLGTMIRRLEPAKNNTLLVKGDIC
                     NDLYLIETGLLACFDTEKNKKYCTWLMTSGDFVTAVDSFNNQRPSTETIIAQTDSVLW
                     AITRQQLEELTARFPEFGVIRQKLTDKYHIQSRVMDAKRKRPPEQFYQYLRENYPYIV
                     DNAPASALASLMGITRTTYYKIVKKQRRKR"
     gene            3372..>3665
                     /locus_tag="VKQ52_07205"
     CDS             3372..>3665
                     /locus_tag="VKQ52_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87007.1"
                     /translation="MSKLFILLAGTLLLSHSNFITSPFIRSSVELRSPAAPAGNKEPD
                     LERCCKKILKAGPRIFYLGDRSGRMRFRLLGIYAHGPALFFLLRLNNRSALDYD"
BASE COUNT          847 a         1157 c          871 g          790 t
ORIGIN      
        1 cctctgtcat gtggcctccg gcgccgatga tgtccggtgc tatatatcgg aactctatgc
       61 ccggcccttt caagcccagg acatcgaaaa gagaaaaaaa gtactggaag gactatacga
      121 tccgaaagac aatttgcaaa aactgctgca cgccctctct attcaataga aagactcgca
      181 ttatcgatca cctgcccccg ctcggtccgc accatccgcg ccgggaattt attgatcacc
      241 cggtagtcat gggtggccat gatgatcgtc gtcccatagt cgcgggagat ctggaacaac
      301 agctgcatga tctcatcgga ggtctcgggg tccaggttgc ccgttggctc atcggccagg
      361 atcagcttgg gcgaattgag cagggcccgg gcgatgtcca cccgctgctg ctccccgccg
      421 ctcatctcga agggcatctt aaaacccttt gccttcagcc ctaccttgtc cagcacatcg
      481 gccaccttct catccatcag ccgcatatct ttccagcccg tcgcccgcag gacgaacttg
      541 aggttatcat tgacgctacg gtccgtcagc agctgaaagt cctggaacac cacgccaagg
      601 gtgcgccgca gatacggaac cttcttccaa tccatctccc gcagatcgaa tccggctacc
      661 tggccctccc cctctctcaa cggcagatcg ccatacaggg tcttcagcag actggactta
      721 cccgtacccg ttttaccaac gagataaacg aactggcctt tgtcgacaga gagattgacg
      781 tcctgcagga tgagattggc gctttggtag atgttggcat gttttaattc cacgatgatt
      841 tcggccatgg caatgattgg attaagtgca acgggatggg ttcaccgcaa aaacaaccca
      901 tccccgctgc actctaatga tgggccttcg gccggcggcc cggatggtcc gccttgttga
      961 tccaaacaaa aatagggaaa aatcacttca taacgggccg ctcgatcttt tccggcgccc
     1021 cctcgcccct gtcggccctg agcatcagcc ggagagtgga atcgttggcg aagtaagcca
     1081 tcatccaatt caggaaaacg atgatccggt tcttgacgtt caacaacagc atcaggtgca
     1141 cgaacatcca ggtcagccag gcaaaaacgc cctggaaaga gagaccggga agatcgacaa
     1201 cggccttccg cttcccgacc gtggccatcg tgcctttatt gtgatattcg aattcgatcc
     1261 gtgtcctgcc cttcagttcc cgtttgaagt tgatcgcgat atgcgcagcc tgggcgatcg
     1321 ccacattggc cagctgcgga tgaccgttag gatatttggg cgtgggcata taggcgatat
     1381 cgccgatggc atataccccg tccaacccct ccacttcccc gaaccggttc gtcttgagcc
     1441 ggtttccccg caccatcact gaagcgggga tcccctcgag gacattgccg gtgaccccgg
     1501 cggcccagat aaggttccgg gcgacgatcg tctcccctcc cttcagcctc accgtattgc
     1561 cgtcataatc ctccacggtt gttcccgtac ggaccatcac ccccaactgc tccaggtact
     1621 tccgggaagt ggcccgggag acctcgctca tggaattaag cgtattgggc gatccttcga
     1681 tcaggtagat cgtcagccgc gaaaaatcca cctccgggta atccttgggc aggatattct
     1741 tcttcatctc ggccatggcc ccggccagct cgacaccggt aggccctccg ccgacgatca
     1801 cgaaattcag cagcgactgc cgttcatcgg gccccgcctc gatcgtgtcc tcaaaggtct
     1861 gcaggatccg gttgcgcaat gccatcgcat tgggaacgga tttcatcggg aaggaattgg
     1921 ccctgatccg ttcgttaccg aaataattgg tcgtacaccc gatacaaagg acaagccggt
     1981 cgtaggtgat atcccctatc gtcgtctcca gcattttgtt gactacgtcc acccgcaaca
     2041 cgtcggcgat ccggatggta acattatgcg tgtgctgaaa gaccttgcga agcggaaaag
     2101 agatgctgga cggttcgagc cgggccgtcg ccacctggta catcagcggc tgaaactggt
     2161 ggtaattgtg ccggtcgata agaaaaacat cgtacgcagt gttgttgagg ctcctggcga
     2221 tccgcaaccc cgcaaaaccg gcccccacga taacgacccg ttgcctcttg ctgttctctg
     2281 gtgacatagt gaagaagttt aaatgccgta tcagcaagca aagttacagt atccggggac
     2341 aaaggtacca attgccggct aaaccccaat caggaatagg aaatctacca tcccggccgg
     2401 gattcccgca actgcgggat catttcatcc gggcacattt attttaaagt atacaatacc
     2461 gttaccaaaa aatatacaac accgttacca atggaatacc cgctgtccca attacaactt
     2521 caccatcccc tctccgggga gtttatccac accctcggca cgatgatccg gcggctcgaa
     2581 ccggcaaaga acaataccct cctcgtaaaa ggcgatatct gcaacgacct ttatcttatc
     2641 gaaacaggac tgctcgcctg cttcgatacg gaaaaaaaca aaaagtactg cacctggctg
     2701 atgacaagcg gcgacttcgt caccgcggtc gacagcttca acaaccagcg gccgtccacg
     2761 gagaccatca tcgcgcagac ggacagcgtc ctctgggcga tcacgaggca acagttggaa
     2821 gaactcaccg cccgcttccc ggagttcggc gtcatccggc agaaactgac cgataaatac
     2881 catatccaga gcagggtgat ggacgccaaa cgaaaacgcc cgcccgaaca gttctatcaa
     2941 tacctccggg agaactaccc atacatcgtg gataacgccc ccgcaagtgc actcgcctcc
     3001 ctgatgggca tcacccggac cacctattat aagattgtca aaaagcagcg ccggaaaagg
     3061 tgatatcgac cggcccggcc ctgccctgat ctaccgaccc agcccgacct cgatctgccg
     3121 gccccggcaa gcgacctgcc cggcccatcc cctcctgctg gccccgaccc gatctggcgg
     3181 cccagtccaa ccccccgtcg ccggccccca cctgctgtca gatttctgac accatactgt
     3241 ccattttctt acactgccct gtcaatttta ttacacgctg atgttcaatc tattacacac
     3301 ccattttgtc ctttttctcc tcccatttac taggtttgta ctgtcctttt ttcaaaccaa
     3361 aaatcaagaa catgtccaaa cttttcatcc tgctggcagg aaccctcctg ctcagtcatt
     3421 caaacttcat cacttctcca ttcatccgat catctgttga attgcgaagc ccagccgccc
     3481 cggccgggaa caaagaaccc gatctggagc gctgctgcaa aaagatccta aaggcaggtc
     3541 cacgcatctt ctaccttggc gatcgcagcg gccggatgcg attccgcctg ctcggcatct
     3601 acgcacacgg ccctgccctc ttcttcctgc tgcggttgaa caaccggtct gcccttgact
     3661 atgat
//
LOCUS       DATGFG010000381         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1521278, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000381 DATGFG010000000
VERSION     DATGFG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521278"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1880)
                     /locus_tag="VKQ52_07210"
     CDS             complement(<1..1880)
                     /locus_tag="VKQ52_07210"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HLZ87008.1"
                     /translation="MMNLQQKDVSLGEAHGSIDTTTRKGPWWRQLFVFFGPAYLVSVG
                     YMDPGNWATDLAGGSRFGYSLIWVLLMSNGMAILLQNLAARLGIVRGRDLAQANREVY
                     PAYINIPLYVLAEIAIAACDLAEVLGMAIGIQLLTGLPLVYGVCITVLDTFLLLFLQR
                     MGMRRMEAFVVGLIAIVGLSFLVEILLARPAAADILSGFVPRLQGREALYIAIGIIGA
                     TVMPHNLYLHSALVQTRKIRPGAAHVRRALKINFLDSAVALNLAFLVNAAILILAATV
                     FFKTGRTDVASIKEAYRLLPMLGTQLAPKLFAIALIASGQSSTLTGTLAGQIVMEGYL
                     QLRINPWVRRLLTRLLAIIPAVVVILLFGENEVDNLLVLSQVILSVQLGFAVIPLIHF
                     VSDKATMGEFIIKPIIKTLAWGTALLLLYLNIKLVVEEVAPVMAAPGMAGWKLLITIA
                     GLGAVALLAYIALHPWIERQVRRKAPQLHPLPDPLTDWTPPAFRHIAVALDFSEHDEK
                     LLAYAVGQGGTAANYLLIHVVESASAQLLGKESDDYETRKDQERLDRYRAILGEKGLQ
                     SHTVLGFRHRSREIARIAREQQADMLVIGSHGHTGLKDLIYGETVNAVRHELKIPVLV
                     VS"
     gene            complement(1852..2532)
                     /locus_tag="VKQ52_07215"
     CDS             complement(1852..2532)
                     /locus_tag="VKQ52_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HLZ87009.1"
                     /translation="MKVSASKENYLKAIFHLQQEQGLVTTNALAAALRTRPASVTDML
                     KKLKEQKLLIYERYKGFKLNNEGRKAAVQVIRKHRLWEYFLVKKLQFGWDEVHDIAEE
                     LEHITSRALTDRLDAFLGYPETDPHGDPIPDSQGRLLVRRQVCLADLPLNKVVQVSGI
                     ASQTSEMLEVLQHNHIRLGTRLEIRKRFPFDDSLEVKVRNRPPVTLSARVAKNVLVND
                     DEPPAERR"
     gene            2707..3693
                     /locus_tag="VKQ52_07220"
     CDS             2707..3693
                     /locus_tag="VKQ52_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87010.1"
                     /translation="MFKYLLLIGLVLFISCKSKKVSLAVNDEKVNISDFLEFFQPMKL
                     PYQVTDTILKRKEPETAVINAKLFARFVPDSIVGHLFGKDLHPHLYAIGKISVPDAEQ
                     YLFVKMTAKERRALYILCFDKKNHFAAARPMLYSDNEPGTSGQAAMDNKYTLTILHQR
                     KAADGQILYHKDAYIFNGDVGFMLILTESNEGRPKIPPLYNPIDTLPRKHKFSGDYAQ
                     DKRNIVAIRDGKDPSRFWFFIHFEKDEGTCKGELKGEARFVAPGVARYRAYSDPCAIE
                     FTFIPEGVSLKEQGGCGVHRDIKCFFEGYFLRRKTKEKPEEHKRPKIRVNET"
BASE COUNT          826 a         1089 c         1054 g          767 t
ORIGIN      
        1 aaactcacca ccaataccgg gatcttcaac tcatgccgca ccgcgttcac ggtctctcca
       61 tagatcaggt cctttaaacc cgtatgtcca tgcgagccga tgaccagcat gtccgcctgt
      121 tgttcccggg cgatgcgggc gatctcccgg ctgcgatggc ggaagcccag caccgtatga
      181 ctctgcagcc ctttctcgcc aaggatggcg cggtagcggt cgagccgctc ctggtctttc
      241 cgggtctcgt agtcatcgct ttcttttccg agcagctggg ccgaggcgct ttccaccaca
      301 tggatcagca ggtagttggc ggccgttccg ccctggccca cggcataggc cagtaatttc
      361 tcatcgtgtt cgctgaagtc aagcgccacg gcaatatgcc ggaaagccgg cggcgtccag
      421 tccgtcagcg gatcgggcag gggatgtaac tggggagcct tccgccgaac ctgtctttcg
      481 atccagggat gcagggcgat ataggcaagc agggcgacgg cgccaagccc ggcgatggtg
      541 atgagcagct tccagccggc cattcccggg gccgccataa cgggcgccac ctcttccacc
      601 acgagtttta tattgagata gagcagcagg agggcggtgc cccaggcgag cgttttgata
      661 atgggcttga tgatgaattc ccccatcgtc gccttgtcgc tgacgaaatg aataagcggg
      721 atgaccgcga atcccagttg gacactcagg atcacctggc tgagcacgag caggttatcc
      781 acttcgttct ccccgaagag gaggatcacc actacggcgg gaatgatggc cagcagccgg
      841 gtcagcagcc gtcgcaccca gggattgatc cgcagttgca gatagccttc catcacgatc
      901 tggcccgcaa gggtgcccgt gagcgtggag ctctggcccg aggcgatcag ggcgatcgca
      961 aacaactttg gcgccagttg cgtacccagc atgggcaaga gccggtaagc ctccttgatg
     1021 gaagctacat cggtccttcc tgtcttgaag aaaacggtgg cggccaggat gaggatggcg
     1081 gcattgacaa ggaaggcgag gttcagggcc acggcactgt ctaaaaagtt gatctttagc
     1141 gccctgcgaa cgtgtgccgc tccggggcgg atctttcttg tctgtacgag ggccgagtgc
     1201 agatagaggt tgtggggcat gaccgtggcg ccgatgatgc cgatggcgat ataaagggcc
     1261 tccctgccct gcaaccgggg gacaaaacca ctcagaatat ccgccgccgc cggccgggcc
     1321 agcaggatct ctaccaggaa ggagagtccg acgatcgcga tcagcccgac gacaaaggcc
     1381 tccatccggc gcatccccat ccgctggagg aaaaggagta aaaaggtatc caggacggtg
     1441 atgcatacgc catagacgag gggcagaccc gtgagcagct ggatcccgat cgccataccg
     1501 aggacctcgg ccaggtcaca ggccgcgatc gcgatctccg cgagcacata cagcgggata
     1561 ttgatatagg ccggatagac ctcgcggttg gcctgggcca ggtcgcgtcc ccggacaata
     1621 cccagccgcg cggcgaggtt ttgcaggagg atggccatgc cgttgctcat cagcaggacc
     1681 cagatcagcg aatagccaaa acggcttcca cccgccagat cggtggccca gttgcccggg
     1741 tccatatatc ccacgctcac caggtaggcc ggaccgaaaa aaacgaaaag ctgccgccac
     1801 cacgggccct tgcgcgtggt ggtgtcgata ctgccgtgtg cttcaccgag actaacgtct
     1861 ttctgctgga ggttcatcat cgtttacaag gacatttttc gctaccctgg cgctcagggt
     1921 aaccggcggc cggttacgga ccttcacttc cagggagtcg tcaaaaggaa atctttttct
     1981 tatctcgagc cgggtgccca gccggatatg gttatgctgc aacacttcca gcatctcgga
     2041 ggtctgactg gcgatgcccg acacctgcac caccttgttc aggggcagat cggccaggca
     2101 aacctgccgc cgtaccagca gccgcccctg gctgtcgggg atcgggtcgc catggggatc
     2161 ggtctccggg tagcccagga aggcatccag ccggtcggtc aatgcccggc tggtgatatg
     2221 ctccagctct tcggcgatgt catgcacttc gtcccagccg aactgtaatt tctttaccag
     2281 gaaatattcc cagaggcgat gcttgcggat cacctggaca gcggccttcc gaccctcgtt
     2341 gttcagcttg aatcccttat accgctcata gatgagcagt ttctgctcct tcagcttctt
     2401 cagcatgtcg gtgaccgaag caggccgggt ccgcagcgcc gccgcgaggg cattggtcgt
     2461 gacgaggccc tgctcctgct gcagatgaaa aatggccttg agataattct ccttgctggc
     2521 agacactttc atattcgcct tgactaaatt aaaatttaga caaatctaaa tttaaaaatc
     2581 cacatttccg gcgttatttt ttcaaaaaaa gccctccggc ttgtcgcctt ctgtcgccga
     2641 atgtttattt tagccgggta aatctggcaa cggtccggag ggccgttctg cgataattaa
     2701 ccaagtatgt tcaaatacct gttgctgatc ggcctggtgc tgtttatttc gtgtaagagc
     2761 aagaaggttt ccctggcagt gaacgatgag aaggtgaata tttccgattt cctggagttc
     2821 tttcaaccca tgaaactgcc ctaccaggtg acggatacca ttttgaagcg aaaagagccg
     2881 gagaccgctg tcatcaacgc caaactgttc gcccgttttg tgccggactc catcgtcggc
     2941 catctcttcg gcaaggatct gcatccccat ctctatgcta tcggaaagat cagcgtcccc
     3001 gacgccgagc agtacctgtt tgtcaagatg acggcaaagg aacgccgggc cctctacatc
     3061 ctctgtttcg ataagaagaa ccattttgcc gcggcccgtc ccatgctcta ttcggataac
     3121 gagccgggta ccagcggcca ggcggcaatg gacaataaat atacgcttac catcctccac
     3181 cagcggaaag ccgcagatgg ccagatcctc tatcacaagg atgcctacat cttcaacggc
     3241 gacgtcggtt ttatgctgat cctcaccgaa tccaacgagg gcaggccaaa gatcccgccc
     3301 ctctataatc cgatcgacac cctgcccaga aagcacaaat tctccggcga ctacgcgcag
     3361 gataagcgga acatcgtcgc catccgggac ggaaaagacc cttcccgctt ctggttcttt
     3421 atccatttcg agaaggatga aggaacgtgc aagggcgaat tgaagggcga agccaggttc
     3481 gtcgctcccg gtgtggcccg ctatcgggct tattccgacc cctgtgcgat cgaatttacc
     3541 tttattcccg agggagtatc cctgaaggaa caggggggct gcggcgtcca ccgcgacatc
     3601 aaatgtttct tcgaagggta tttcctacga aggaagacaa aagaaaaacc ggaagaacac
     3661 aagcgaccga aaataagggt caatgagaca tgatcggcca tgaacagccg ggagcgggct
     3721 gcactgcggg caagtc
//
LOCUS       DATGFG010000382         3517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_938754, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000382 DATGFG010000000
VERSION     DATGFG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938754"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..524)
                     /locus_tag="VKQ52_07225"
     CDS             complement(63..524)
                     /locus_tag="VKQ52_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HLZ87011.1"
                     /translation="MFNGKNKSDSFEAGGSAPVSTSASLIGAGTTMKGDITSNGDLRI
                     DGTLVGNIHCSAKVVIGANGNVQGDINGQTADIMGKVTGTIKVKDLLLLKGGSTLNGN
                     IQAAKLQIEPTANFNGECHMTAAATGSNTAPAGKGSPEIKKEIKNVETAIA"
     gene            complement(536..931)
                     /locus_tag="VKQ52_07230"
     CDS             complement(536..931)
                     /locus_tag="VKQ52_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87012.1"
                     /translation="MARFDANSTFKRLQNRYRLVVMNDDTYEEVVTFKLSRLSVYTVL
                     CTIFVLLTGLTVALIVFTPLKLYIPGYGDVNNTKDLRELKIRTDSLEQEVRYKDQYLE
                     SIRNVLQGNTTVKLDTTTLKIPKPEKIND"
     gene            1149..>3517
                     /locus_tag="VKQ52_07235"
     CDS             1149..>3517
                     /locus_tag="VKQ52_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87013.1"
                     /translation="MAYRKILLSGVFSCLFLALLAQDGVDYTLTKPKKFENRVLAAEN
                     SNDGKKIKRVRRFIQNTITHYNYYFNANEKIKMILARAKASFRDDYTRLLPFYNYSLD
                     ATAAQKRELDSVIYKCTMGILIHDTRDDWIDNLYLLIGRTYYLKKDFDSAFITFQFVN
                     WAFAPKDKEGNDQPIGSNYNKDEGTSADKVATKENPNIGQKVFSVPPSRNDALIWKIR
                     TYIAKDQYPQASGLIEILLHDPLFPPRLEPSLQEVRALWFYKQNIYDSAAIFLEKALP
                     AAEGREETARWEYLIAQLYERTGDSYHARLFYEKTVNHTYNPILEIYARLNAIRQNKE
                     GGEDYIARNIEALVKMAHRDRYESYRDIIYFTAAQMELERNNKPGAISFLRQCIKYSV
                     GSANNQRNKAFLQLANLSFEGKDYRSAKNFYDSLNLTSADRETLGDISWLPDRKAALA
                     VIVKQLQVIDRQDSLQRIAAMPAQQRDAYIKKLVRYLRRQQGLRDEADSGFSMSPLNN
                     NSNAAPDLFASSGNTADWYFDNQALKAKGYSDFRTKWGNRPNVDNWQVSSLARNNLGP
                     RNGARNGLDLGDSASRVTSLNSNVDFKTLLANLPLTPDKMKKSMDSIESAFFTLGRTY
                     QDGIPDYILSINAYDTLLAKFPDTRHWEESLFNLYYCYKKLGDEENAQHILDLLNRKF
                     PKGAFTSRLANPDSVEGAAGSTKARATQQYEKVYNDFIEGRFEEAVAEKKVADSLYGD
                     KYWTPQLLYIESVYFIRSRQDPEARTVLNGIIAKWPKTPMAAKAATMLDV"
BASE COUNT          899 a         1103 c          791 g          724 t
ORIGIN      
        1 tcaggcaggg gcaatttacc tccaaccata atagcttctc aatggcctcg gtgaaccgcc
       61 cgctacgcta tagcggtttc cacattcttt atttccttct ttatttccgg ggaacctttg
      121 ccggcaggag ccgtgttcga acccgtggca gccgccgtca tatggcactc gccattgaaa
      181 ttggccgtag gctcgatctg taatttcgcg gcctggatat ttccattcaa cgtactgccg
      241 cccttcagca gaagcaggtc ctttaccttg atggtcccgg tcacctttcc catgatatcc
      301 gccgtctggc cgttgatatc cccctgcaca ttgccattgg ccccgataac gaccttggcc
      361 gaacaatgga tgttcccaac cagggtacca tcgatccgca gatcgccatt gctggtgata
      421 tcccctttca tggtggtacc ggcgccgatc aggctggcgc tcgtgctcac gggcgcagac
      481 ccgcccgctt caaatgaatc agatttgttc tttccgttaa acataaatat ggattttagt
      541 catttatttt ctcaggtttc gggatcttca aagtcgtcgt atccagcttc actgtcgtat
      601 tcccttgtaa cacattgcga atactctcca ggtactggtc cttgtaccgc acctcctgct
      661 ctaacgaatc agtgcgtatt tttagttcac gcaaatcctt ggtattgttc acatccccat
      721 acccggggat atacagcttc aggggcgtaa aaacgatcaa cgccaccgta agccccgtca
      781 acaatacaaa aatcgtgcag agaacggtgt aaacactcaa ccgggacaat ttaaacgtga
      841 ccacctcctc ataggtatca tcattcataa ctaccaggcg gtagcggttc tggaggcgtt
      901 taaaggtgga attggcatca aatctggcca tacaggatta attggtacaa tcttgggtct
      961 ttcaacctaa agataacaaa ataaatttcc ggtaaaagag ttatttatgc ggcggcggcg
     1021 ctttatttgc aaaacgtaaa ccgcctccgc ctcatggctt atgattggcc ttcggccgac
     1081 ggaccgaggg cccgtcttgg gcggcgacgg cgctttattt gcggaccggt ttctcaactt
     1141 tgatcatttt ggcctatcgt aagattttgc tgtcaggggt attttcatgt cttttcctgg
     1201 ccctcctggc ccaggacggt gtcgactata ccctcaccaa accaaaaaaa ttcgagaacc
     1261 gcgtccttgc cgccgaaaac tccaacgacg gaaaaaagat aaaaagggtc cgccgcttca
     1321 tccagaacac catcacccac tacaattact acttcaacgc caacgaaaag ataaagatga
     1381 tcctcgcccg ggcaaaggca tccttccgcg acgactatac ccggctcctc cccttctata
     1441 actatagcct cgacgcaacg gcggcacaga aacgcgaact ggactccgtc atctataaat
     1501 gtaccatggg catcctgatc cacgacaccc gcgacgactg gatcgataac ctctacctgc
     1561 tgatcggcag aacctactat ctgaaaaaag atttcgactc ggcctttatc accttccagt
     1621 tcgtgaactg ggccttcgcg ccaaaagaca aggaaggaaa tgaccagccc atcggcagca
     1681 actacaacaa ggatgaaggc accagcgccg acaaggtcgc cacaaaagag aaccccaaca
     1741 tcggccaaaa agtattctcc gttcccccga gccggaatga cgcgctcatc tggaagatcc
     1801 gcacctatat cgccaaagac cagtaccccc aggcctcggg actgatcgag atcctgctcc
     1861 acgatcccct cttcccaccc cgcctcgaac ccagcctcca ggaagtaaga gcgctctggt
     1921 tctataaaca gaacatctac gacagcgccg ccatcttcct ggaaaaagcc ctgcccgccg
     1981 cggaaggccg cgaagaaaca gcccgctggg aatacctcat cgcccagctg tatgaaagaa
     2041 caggcgacag ctaccacgca agactcttct acgagaagac ggtcaaccat acctataacc
     2101 ccatcctgga gatctatgcc cgcctgaacg ccatccgcca gaacaaggaa ggcggcgaag
     2161 attatatcgc ccgcaatatc gaagccctgg tgaagatggc ccaccgcgac cgctatgagt
     2221 cctaccggga tatcatctat tttacggccg cccagatgga actggaaaga aataacaaac
     2281 cgggcgccat ctccttcctg cgccaatgca taaaatactc cgtcggctct gccaataacc
     2341 agcgtaacaa ggcctttctc cagttggcca acctctcctt cgaaggaaag gattaccgct
     2401 ccgcaaagaa tttctacgat agcctcaatc tcacctccgc cgaccgggaa acccttggcg
     2461 acatcagctg gctccccgat cgcaaggcag ccctcgccgt catcgtcaaa caattacagg
     2521 tgatcgatcg ccaggatagc ctccagcgca tcgccgccat gccggcccag caacgggacg
     2581 cctatataaa aaaactggtc cggtacctcc gccggcagca ggggctccgc gacgaggccg
     2641 acagcggctt ctccatgtcc ccgctgaaca acaactccaa tgccgcgccc gatctcttcg
     2701 cctccagcgg gaacacggcc gactggtatt tcgataacca ggcgctaaaa gcaaaaggct
     2761 attccgactt ccggacaaaa tggggcaacc ggcccaatgt cgataactgg caggtctcct
     2821 ccctggcaag aaataacctc ggcccccgca acggcgccag gaacgggctc gacctgggcg
     2881 atagcgccag ccgggtcaca tcactgaatt cgaacgtcga cttcaagacc ctgctggcca
     2941 acctgccact cacgcccgat aaaatgaaga agtcgatgga ctccatcgaa agcgccttct
     3001 tcaccctggg ccgcacctac caggatggca tacccgatta catcctttcc atcaatgcct
     3061 acgataccct gctggccaaa ttccccgaca cccgccactg ggaggaatct cttttcaatc
     3121 tctactactg ctataaaaaa ctgggcgacg aagagaacgc ccaacacatc ctcgacctgc
     3181 tcaaccggaa gtttcccaaa ggcgccttca cctccaggct ggccaatccc gactccgtcg
     3241 aaggcgccgc gggcagcacc aaggccaggg ccacccagca atacgaaaag gtctataatg
     3301 actttatcga aggccggttc gaagaagccg tcgcggagaa aaaagtggcc gacagcctct
     3361 atggcgacaa atactggact ccgcagttgc tctacatcga atccgtttat ttcatccgga
     3421 gccggcagga cccggaggca agaacagtgc tcaacggcat catcgccaaa tggccaaaaa
     3481 cacccatggc cgcaaaagcc gccacgatgc tcgatgt
//
LOCUS       DATGFG010000383         5235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_25468, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000383 DATGFG010000000
VERSION     DATGFG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25468"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VKQ52_07240"
     CDS             complement(<1..315)
                     /locus_tag="VKQ52_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87014.1"
                     /translation="MLLIDQNIPLIVQARVQSTRYPGKVLKPFAEGRSLLEFQLMRLK
                     EAFPESPLVVATSTAAADEPIVQLAKAMGVFSFRGDEQDVLKRFVNCCQYFGFNGQII
                     RIC"
     gene            complement(386..2089)
                     /locus_tag="VKQ52_07245"
     CDS             complement(386..2089)
                     /locus_tag="VKQ52_07245"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ87015.1"
                     /translation="MPALLSIQQLGIGFVTGDGMAPGVEELSLEVARGEIVALVGESG
                     SGKSITSLSILRLLPTPPARYLSGKILFTGCDGQTVDLLQLGEEALRELRGAEIAMIF
                     QEPMTSLNPVFTCGNQVAEAIRAHRKVSAEEAREQAIALFGRVRLPDPEGIFHRWPHQ
                     LSGGQKQRVMIAMAMSSRPSLLICDEPTTALDVTVQKTILELIRQLQETEDMGVIFIT
                     HDLGVVAEIADRAAVLYKGKVVEQGPVQELFRHPRHPYTKGLLNSRPALHLKGERLPT
                     VSDYLEGKEPVKAVTREAAPVVTAGGETLVRVEDLRVWYPAKKTLLGKVLQYNRAVDG
                     VSFDVYKGETLGLVGESGCGKTTLGRALLRLVEPTDGRVFFEGRELGAVSAGELKRLR
                     SRMQLIFQDPYSSLNPRMTIGAAIAEAMKVHGLGSGEKQRRSMVIELLEKVSLSAAHV
                     DRWPHEFSGGQRQRIVIARALALRPSFIVADESVSALDVSVQAQVLNLLNDLKKEFGF
                     TIVFISHDLSVIRYISDRIVVMNKGRIEELGDAETIYYHPRSEYTKKLIAAIPRLAAA
                     D"
     gene            complement(2091..2861)
                     /locus_tag="VKQ52_07250"
     CDS             complement(2091..2861)
                     /locus_tag="VKQ52_07250"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HLZ87016.1"
                     /translation="MKTKEEIVNNWLPRYTGEKLENFGEYILLTNFDNYVNLFAEWHN
                     VPIIGEHKPMQCATAGDITIINFGMGSPGAATIMDLLTAIHPKAVLFLGKCGGLKKRN
                     KLGDLILPIAAIRGEGTSNDYFPPEVPALPAFALQKAISTTIREYAVDYWTGTVYTTN
                     RRVWEHDETFKEYLQKIRAYAIDMETATIFSVGFYNKIPTGALLLVSDQPMIPEGVKT
                     AESDLKITGEFVEKHIRIGIDSLKQLINNGLTVRHLRF"
     gene            2891..3334
                     /locus_tag="VKQ52_07255"
     CDS             2891..3334
                     /locus_tag="VKQ52_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HLZ87017.1"
                     /translation="MIKIYYPSYPFKIREPERKKEEIWDELRKQWVRLTPEEWVRQNF
                     IQYLLLVKNYPGSYMAVERRMKLGELNKRFDLLVFDKAAQPWMLVECKAMEERLDSRV
                     LWQVLRYNMAIPIKYLVITNGEECHAFIKGLTDFENISALPAYSE"
     gene            complement(3354..>5235)
                     /locus_tag="VKQ52_07260"
     CDS             complement(3354..>5235)
                     /locus_tag="VKQ52_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ87018.1"
                     /translation="RLDNDKEYRVSGVFHDMPENATWRLDWIASLQRNYNDHKAEGWP
                     DSWGANSINTFVLLSPRADVAAVGKQLYPFIANKDKGAIARPLLFSANDWHLRDHFED
                     GKIAGGQIEYVRLFVVIAWIILLIACINFMNLATARSDKRSREVGVRKVMGAERRGLI
                     GQFIGEAMVMALLSVITGVILISLTIPAFALLIGRPLDLGLTKPMHIGVLAAIALVCG
                     LVAGSYPALYLSAFKPAFVLKGIRTQGGGAAFIRKGLVVAQFTVAVALIISTAIIYRQ
                     IEHIRNRDLGYNHHNLVAAAAQGEIPQHFSQVRQDLINTGVVEKAALCNYNTLWGGNN
                     GSGAKWDGKDASQDPLISYRAVTPGFVETVRMHLLEGRDFRADMPEADSMHVLVTETF
                     AKMMGKGNAVGKRIWWDNNPPYTVVGVVKDFVFGNMYGKPEPVIFFCLAKNNISDSRY
                     IYVQMKPNVPAETAIAKMTAVLKKDNPAYPVNYSFVSDDFNSLFRTEKLMGQLSRLFA
                     ALAILISCLGLFGLSAYMAERRVKEIGIRKVLGASITGLTGLLSKEFLQLVGLSALIA
                     FPLAWWAMDRWLQQFTYRIGIEWWIFLVAGLAALAIALMTVSFQSVRAAISNPVKALR
                     SE"
BASE COUNT         1148 a         1651 c         1314 g         1122 t
ORIGIN      
        1 gcaaatacgg atgatctgtc cattgaatcc aaaatattga cagcagttga caaatcgttt
       61 taatacgtcc tgctcatccc cccggaagct aaatacaccc attgctttag caagctgcac
      121 gatgggctca tccgcggcgg ccgtcgaggt agcaacaaca aggggtgatt cggggaatgc
      181 ctcctttagc cgcatcagct gaaattcgag cagggaacgg ccttcggcaa atggcttcag
      241 gaccttgccg ggatagcggg ttgattggac tctcgcctgt acgatcagcg ggatattctg
      301 gtcgattaag agcacggcga atagactaat gatccggggg aaagattaat ggcgcaacgt
      361 cttttaattc ttttttaatg tcgatttagt ccgcggcggc cagtcgggga attgccgcta
      421 taagcttttt agtatactca ctccggggat ggtagtagat cgtctctgca tcgcccagct
      481 cttcgatgcg acccttgttc atcacgacga tacggtcgct gatataccga atgaccgaca
      541 ggtcatgcga gataaagaca atggtaaacc cgaattcctt cttcagatcg ttcagcaggt
      601 tcagcacctg cgcctgtacg cttacatcca gcgccgacac cgactcgtcg gcaacgataa
      661 aggatggccg cagcgccagc gcgcgcgcga tcacgatccg ttgccgctgg ccgccggaaa
      721 attcatgcgg ccagcggtcg acatgcgcgg cgctgagact aaccttctcc aacagttcaa
      781 tcaccatgct ccgccgctgc ttttcgcccg agccaagccc atgcactttc atcgcctccg
      841 caatagccgc accgatggtc atccgggggt tcagcgatga gtacgggtcc tggaaaataa
      901 gttgcatcct gctccgcaac cgcttcagtt ccccggcgga tacggccccc agctcacgcc
      961 cttcgaagaa gacccggccg tccgtcggct ccaccagccg cagcaaggcc ctgcccaggg
     1021 tagtcttccc acaacccgat tcgcccacca gtccaagggt ctcccccttg tacacgtcga
     1081 aactaacgcc atctacggcc ctgttgtact gcagcacttt tcccagcagg gtttttttgg
     1141 ccgggtacca gacccgcagg tcctccaccc gcaccagcgt ctcgcccccg gcagtcacca
     1201 ccggcgcggc ttcccgcgtc actgccttca ccggctcctt cccctccagg tagtcgctca
     1261 cggtcggcaa ccgctccccc ttcagatgga gggccggccg gctgttcaac aaccccttcg
     1321 tataaggatg cctgggatgc cggaacaact cctgcaccgg accctgctcc accacctttc
     1381 ccttgtaaag cacggcggcc cggtccgcga tctccgccac gacgcccagg tcatgcgtga
     1441 taaagatgac ccccatatcc tctgtctcct gtaactgacg gatcaattcc aggatggtct
     1501 tttgtaccgt aacgtccaga gccgtcgtcg gctcgtcaca gatcagcagt gaaggccggc
     1561 tgctcatggc catcgcgatc atcacccgct gtttctggcc cccgctgagc tggtggggcc
     1621 accggtggaa gattccttcc gggtccggca gccggacccg cccgaaaagt gcaatggcct
     1681 gctcccgcgc ttcctccgcc gacactttcc ggtgcgcccg gatggcctcg gccacctggt
     1741 taccacaggt aaagacagga ttgagggagg tcatcggctc ctggaagatc atcgcgatct
     1801 ccgccccccg cagctcccgt agcgcctcct ccccaagttg taaaagatcg accgtctgcc
     1861 cgtcgcaacc ggtgaaaagg atcttcccgg atagataccg cgccggcggg gtcggcaaca
     1921 gccgtaaaat cgaaagggaa gtgatcgact tccccgaacc ggattctccc accagggcaa
     1981 cgatctcccc ccgcgccacc tcgaggctaa gctcctcgac gccgggcgcc ataccgtccc
     2041 cggtgacaaa cccgatgccc aattgttgaa tggataacag ggccggcatg ctagaatctt
     2101 agatgtctta ccgtaagccc attattgatc agctgcttca aagagtcgat gccgatcctg
     2161 atatgtttct ccacaaattc gccggtgatc ttcagatcgc tctccgctgt cttgacaccc
     2221 tcgggaatca tcggctggtc gctcaccagc aacagcgcac cggtagggat cttgttgtag
     2281 aaccctaccg aaaagatcgt cgccgtttcc atgtcgatcg cataagcccg gatcttctgg
     2341 aggtattcct taaacgtttc gtcatgctcc caaaccctcc ggttcgtcgt atacactgtt
     2401 cccgtccagt aatcgaccgc atattcccgg atggtcgtcg agatcgcctt ctgcaatgca
     2461 aaggccggaa gggccggaac ctcgggcggg aaatagtcat tggacgttcc ctccccccgg
     2521 atggccgcga tcggcaggat cagatctccc agcttgttcc gcttcttcaa cccgccgcac
     2581 ttcccgagaa aaagcaccgc cttcggatgg atggccgtca acaggtccat gatcgtcgcc
     2641 gcccccggac tacccatccc gaaattgatg atggtgatgt cccccgccgt ggcgcattgc
     2701 atcggcttat gctcgcctat gatgggcaca ttatgccatt cggcgaataa attgacatag
     2761 ttgtcgaagt tggtcaacag gatatattcc ccaaagtttt ccaatttctc accggtatag
     2821 cggggcaacc agttattgac gatctcttct ttggtcttca tttcgtaaat ttagcaaata
     2881 gttttggcct atgattaaaa tctactatcc atcctacccc tttaagatac gtgaacccga
     2941 aagaaaaaag gaagagatct gggacgaact ccgcaaacaa tgggtccgcc tcaccccgga
     3001 agaatgggtg cgccagaact tcattcaata cctgctgctg gtaaaaaact acccgggttc
     3061 ctatatggcc gtggaaagaa gaatgaaact gggcgaactc aacaaacggt tcgatctcct
     3121 cgtcttcgac aaggccgccc agccctggat gctggtggaa tgcaaagcca tggaggaacg
     3181 cctggacagc cgcgtcctct ggcaggtgtt acggtataat atggctatcc cgataaaata
     3241 tctcgtcata accaatggcg aggaatgtca cgcctttata aaaggcctca ccgatttcga
     3301 gaacatctcc gccctccccg cctattcgga ataacgcctt tgggcggatt tttttactcg
     3361 ctgcgcaatg ccttcaccgg gttcgatatc gccgccctca ccgactggaa gctcaccgtc
     3421 atcagcgcga tcgccagcgc cgccagcccc gccacgagga agatccacca ctcgatgccg
     3481 attcggtagg tgaattgctg cagccaccgg tccatcgccc accaggccag cgggaacgcg
     3541 atcagcgccg acagccccac gagttgcagg aactctttcg acaatagccc tgtcaacccc
     3601 gttatgcttg cgcccagcac cttgcggata ccgatctcct ttacccgccg ctcggccata
     3661 taggccgaaa gtccgaatag tcccagacag gagataagga tggccaatgc ggcaaaaagc
     3721 ctggacagct gccccatcag tttctcggtt cggaacaaag aattgaaatc atcactgaca
     3781 aaactgtagt tgacaggata ggccgggtta tccttcttca gcacggcggt catcttcgca
     3841 atggcggtct ccgcgggaac attcggtttc atctgtacat aaatgtatcg cgaatcacta
     3901 atattatttt tggccaggca aaagaagatc accggctccg gtttgccata catattccca
     3961 aatacaaaat cttttacgac acccaccacc gtatagggcg gattgttgtc ccaccagatc
     4021 cgcttgccga cagcgttgcc cttccccatc atcttcgcga acgtctccgt caccaggaca
     4081 tgcatactgt cggcttccgg catgtcagcc cggaaatccc tcccttcgag gaggtgcatc
     4141 cgtaccgtct ccacaaaacc cggggtgacg gcgcggtaag aaataagtgg gtcctggctg
     4201 gcgtccttgc catcccattt ggcgcccgaa ccattgttgc cgccccagag cgtattgtag
     4261 ttgcagagcg cggctttctc gacaacgccg gtattgatca gatcctgcct cacctggctg
     4321 aaatgttggg ggatctcgcc ctgcgccgcg gccgccacga ggttgtgatg gttatatccc
     4381 aggtcacggt ttcggatatg ttctatttgc cggtaaatga tcgccgtcga gatgatcagg
     4441 gccacggcaa cggtgaattg cgctaccacg agccctttcc ggataaaagc agccccgccg
     4501 ccctgcgtcc ggatcccttt taaaacaaac gccggtttga aggccgatag atacaaagcc
     4561 gggtagctgc cggcgacaag cccgcacacc agcgctatcg ctgcaagcac cccgatgtgc
     4621 atgggtttcg tcagccccag gtccaggggt ctgccgatca ggagcgcaaa ggccggtatc
     4681 gtcaggctga tcagaatgac tccggtgatc accgacaaca acgccatcac catcgcctcg
     4741 ccgatgaatt gtccgatgag cccccgccgc tccgccccca ttaccttccg gacgcccact
     4801 tccctggaac gcttgtcact ccgcgccgtt gcgaggttca taaaattgat acaggcgata
     4861 agcaggatga tccatgcgat caccacaaac aggcgtacat attcgatctg gccgcccgcg
     4921 atcttgccat cctcgaaatg atcccgcaga tgccagtcat tggcactgaa caacaagggc
     4981 ctggcaatcg cccccttgtc tttgttcgcg ataaaaggat agagctgttt cccgacggca
     5041 gctacatcgg cgcgcggcga cagcagcacg aaggtgttga tggaattggc gccccagcta
     5101 tcaggccatc cctccgcctt gtgatcatta tagttgcgct gtaacgacgc gatccaatcc
     5161 agccgccagg tggcattctc cggcatatca tggaagacgc ccgacacgcg gtactccttg
     5221 tcgttatcca gcctg
//
LOCUS       DATGFG010000384         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1511602, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000384 DATGFG010000000
VERSION     DATGFG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511602"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3034)
                     /locus_tag="VKQ52_07265"
     CDS             complement(<1..3034)
                     /locus_tag="VKQ52_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLZ87019.1"
                     /translation="MDLSSGLKSAAASNGAGITFVNRTGSEEKLTYRQLYEGALRVLG
                     GLQQLGLQPGDELVIQIEDSKSFLLAFWGCILGRIIAVPLTAGTRGQHEAKVFTIWKY
                     LSRPFLLCEERQTSIPGRMIVSREIFSWPARGQEAATAGGDIAYIQFSSGSTGDPKGV
                     VLTHSMVACNIADIISSLGIGSSDLLLNWMPLTHDMGIIGFHLTGVFSHIDTVCIPTS
                     AFIRRPLLWMESASRHGATVCYSPNFGLHYFLTALERKPDNVWDLSGIRLIVNGAEPI
                     SIALCQKFTASLGGYGLPENCIVAAYGLAEAAVEVSSTPVGEGLTCHYLDRRELHIGN
                     RVRFVEATSEMAVCHMDVGYPVDNCQLRICGLDNTLFPENVLGEIQIRGGNVTSGYYN
                     NDEANQELFTADGWLRTGDIGFMRAGRLVITGRLKNMMILNGQNYYPADIERAIVENG
                     IAGYGKVAACGARGADQFTETLVVFVAETGADGDPAAIADRIRETVWQKLGIQTDRVI
                     PIRRIPKTTSGKIMHFALAERYMNGEFADEPKGPKAGQEEEVGSFKAMLIGWIREILG
                     SSTVDEDSNLFEMGMDSLTAIQLTNRIYRETQKHLPMGALFQHCTVKGICGLVGRISG
                     AGEPVKPDERGETAYLELSLAQKRIWRECRFYGGSVAYHIPVVHHIEGVVDADLLDES
                     IRLVIGRHEILRTSFVVVEDEPMREVHFRGEAGPRLEYTDLRGRTHRLQRAVSVVETM
                     VNSPFDLASPAQFRMGLIRLEENAYWFAFVIHHILADGWSLGMLFREISIFYNSLAEG
                     VRPLAIHEHPMQFGSYVEWQRRLLSTDTLAAARQYWLNELNDLPEPADLPNHSKNRML
                     GRRINTESYCHLITGERVGLLRKLSLQFETTPFALLMSLLNVLLYRYTNQSDIVTGFD
                     VNGRVSQEMERMAGYTLNTLCLRVSLDGRQSFATAVEAVKEKLYRAFEHQLYPFELLA
                     DHLPQGRTGIENPFFRVLVLYQNFYREEFTLLHNRIEMI"
     gene            3441..>4063
                     /locus_tag="VKQ52_07270"
     CDS             3441..>4063
                     /locus_tag="VKQ52_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87020.1"
                     /translation="MEESAIAEWVAKANGHLLVVKAWITLETLQKFGTCTVLADTDTF
                     FYKDPEPLFTKIDPAHALMHLREYNLFSNPKLSAFFSQRSFTSLDGNSYFLHPGCNMW
                     NSGIIGIHQDNTHLLPEIINLTGQLSFDADYPGLKKLMEQTSFSYFLESRLTGILEAE
                     GYVFHYWFFKYARYLLGAHFNEFQQSDREQFTRLLQETNLTREDFANL"
BASE COUNT         1013 a         1211 c          859 g          980 t
ORIGIN      
        1 tgatcatctc tatccggttg tgaagtaacg tgaactcctc cctatagaaa ttctgataca
       61 atacaagcac cctgaaaaac gggttttcga tcccggttct tccctgcggc aggtgatccg
      121 caagcagttc aaatgggtat aactggtgct caaatgccct gtacagcttc tctttaaccg
      181 cctcaaccgc ggtggcaaag gattgccgcc catccaggct cacccggaga catagcgtat
      241 ttaaggtata tcccgccatc cgctccattt cctgtgaaac cctcccgttg acgtcaaatc
      301 ctgttacgat atcgctttga ttcgtatacc tgtacagaag gacattcaaa agcgacatca
      361 gtaaagcaaa aggggtggtc tcgaattgca gggaaagctt cctcagaagc ccgacgcgct
      421 cgccggtgat caaatgacaa tagctttcgg tatttatccg ccttcccaac atccggttct
      481 tcgaatgatt cgggaggtcc gccggctccg gcagatcgtt cagctcgttc agccaatact
      541 gtcgtgccgc ggcaagcgta tccgtggaca ggagccttct ttgccattct acataggagc
      601 caaactgcat cgggtgctcg tggatcgcca aaggccttac accttctgcc aggctattgt
      661 aaaaaatgct gatctccctg aaaagcatac ccaatgacca accatcggcc aggatatgat
      721 ggatcacaaa tgcaaaccag tatgcattct cctccagccg gatcaggccc attcggaatt
      781 gagccgggct tgcgagatcg aaagggctat tgaccatcgt ctccaccacc gataccgccc
      841 tttgcaatcg gtgggtccga cctctcaggt cggtatattc caaacgagga ccagcctcgc
      901 cccgaaaatg gacttcccgc attggctcgt cttccaccac cacaaaggag gttctcagta
      961 tctcgtgacg cccgatcacc aacctgatgg actcatccag caagtctgca tccaccaccc
     1021 cctctatgtg gtgaaccacc gggatatgat aggccaccga accgccatag aaccggcact
     1081 cccgccagat gcgcttttgc gccagggaca gctccaaata agccgtctcc ccgcgctcat
     1141 ccggcttcac cggttcaccg gcccctgaga tacgccccac caacccgcag atccccttca
     1201 ccgtacaatg ttgaaaaaga gcgcccatcg ggagatgttt ttgtgtctcc cggtagatcc
     1261 tgttggtcaa ttgaatagcc gtaaggctgt ccattcccat ttcaaacaga ttgctatcct
     1321 catccaccgt agaggatccc aatatctccc ttatccagcc gatgagcatc gctttgaatg
     1381 atcccacctc ttcctcctgc ccggctttcg gtcctttcgg ctcatcggcg aattctccat
     1441 tcatgtatct ttctgccagg gcaaagtgca ttatttttcc gctggtggtc ttgggtatcc
     1501 ttcttatggg tatcaccctg tccgtctgta taccgagctt ttgccaaacc gtctccctga
     1561 tcctatcggc aatcgcagcc ggatcaccat cagcaccggt ttcggcaaca aatacaacca
     1621 gcgtctccgt aaactgatca gcaccccggg ccccgcatgc cgccaccttg ccatatccgg
     1681 caatgccatt ctcgacgatg gcccgttcga tatccgcggg ataataattc tggccgttga
     1741 ggatcatcat atttttcaat ctcccggtga taaccagcct gcccgcccgc ataaaaccta
     1801 tatctcccgt cctcaaccat ccatccgccg taaacaattc ctggttcgcc tcatcgttat
     1861 tataatatcc cgacgtaaca tttcctcccc tgatctggat ctcgccaagt acattttccg
     1921 ggaaaagagt attgtccaac ccgcagatac gcaactggca attgtccacc gggtacccga
     1981 cgtccatatg acaaacagcc atttccgatg tagcttcaac aaaccgcacg cgattgccga
     2041 tatggagctc tcttctgtcg agatagtgac atgtcaatcc ttctcccacc ggcgtactgc
     2101 tcacttctac cgctgcctcc gccaatccat aggccgccac tatacaattt tccggcaaac
     2161 cgtacccccc gagggatgct gtgaactttt ggcataaggc gatggaaata ggttcggccc
     2221 cgttgacgat caaccggata cccgacaagt cccaaacatt atccggctta cgctccaatg
     2281 cggtcagaaa ataatgcagc ccgaaattcg gcgaatagca aaccgtggcc ccgtgcctgc
     2341 tggccgattc catccacagc aatggccgcc tgataaaggc agaggtcggt atacaaaccg
     2401 tatcgatatg actaaatacg ccggtcaaat gaaaaccgat gatgcccata tcatgcgtca
     2461 atggcatcca attcaacaac aggtcgctgc tcccgatgcc caggctactg atgatatccg
     2521 ctatattaca cgccaccatc gaatgcgtca gcaccacacc tttcggatca cccgtcgacc
     2581 ccgatgaaaa ttgtatatag gcaatgtccc ccccggcagt cgcagcctcc tgtccccgcg
     2641 ccggccagga gaatatctcg cggctgacga tcattcgccc gggaatcgat gtttgtcttt
     2701 cctcacatag gagaaagggc ctggaaaggt atttccatat tgtaaagacc tttgcctcat
     2761 gctgaccccg ggtacccgcc gtcaagggta cagcaatgat gcgcccgagg atgcagcccc
     2821 aaaatgccag cagaaaactc ttgctatcct ctatttggat aaccagttcg tctcccggct
     2881 gaagccccag ttgctgaagc cctcccaata cccgcaacgc cccctcatat agttgccgat
     2941 aggtcagctt ctcctcggac cctgttctgt tgacaaacgt tatccccgcc ccgttgctgg
     3001 cggcagcact cttcaacccc gatgacaaat ccatggaaaa taattaaaag gaaaacgggt
     3061 acagctatcg ctaacgcagg cgataagctg ccgtcacata tttggtataa tccggttaaa
     3121 gtaaattact tcaaaaacca atataaaatt caacccttag ccgatttcgt aaaaaccgca
     3181 gaacatttag caatccttag ttcccggggt ttttatttgt ctttatcttt attcgatttg
     3241 atatgacaaa cacgactctc atctatctcg ttatcgaaaa cgaaaatact gcgcgcgcaa
     3301 atgaattgat ctattcactg tcttccttaa acgcggtagc gggtacatcg gtgagggaaa
     3361 aattgagaat tatcatttat tccaaccggc ggttcgaatt gccggcatat ctcaatggac
     3421 tcgatattac cttttctata ttggaggaat cggcaatagc cgaatgggtc gccaaagcca
     3481 acggccacct tctggtcgtc aaagcctgga tcactcttga aacactccaa aaatttggca
     3541 cgtgcacagt cctggccgac accgacacgt tcttctataa agaccctgaa ccgctgttca
     3601 caaaaataga cccggcgcat gcccttatgc accttagaga atataacctc ttctcaaacc
     3661 cgaagctgag cgcatttttt tcacagcgga gcttcaccag cctcgacggc aacagctatt
     3721 tcctgcatcc cggctgcaat atgtggaact cggggatcat aggtattcat caggataata
     3781 ctcacctgct tccggaaata atcaatctca ccgggcagct ttctttcgat gctgactacc
     3841 cgggactgaa aaaactgatg gagcaaactt cattcagcta ttttcttgaa agccggctga
     3901 ctggaatact cgaggctgaa ggatatgtct ttcattattg gtttttcaag tacgcgaggt
     3961 atttgctggg cgcgcatttt aatgagttcc aacaatccga ccgcgaacaa ttcacccggc
     4021 ttttgcagga aaccaatcta acccgggaag actttgccaa cct
//
LOCUS       DATGFG010000385         5451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1642871, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000385 DATGFG010000000
VERSION     DATGFG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642871"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="VKQ52_07275"
     CDS             <1..1526
                     /locus_tag="VKQ52_07275"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HLZ87021.1"
                     /translation="NQAFNVENQASTYLNNIEESAKSLQDQEVKITIVNWLLDYIGKA
                     ENNYRLVPTDLGIAEPTLLGQIAQYNQLVLERESNLKTTPAQNPLITRIDGNLDKVRQ
                     NMLQALQNVRTSYIIARDAIQQRGGQLKSQLKSIPGKSMRELNIQRRRKILEDLYSFL
                     LQKRLETSISSASTISNSKVVEPAAGGGLPIKPNRNSLYMTYMLIGLIIPVAIIAIIE
                     VMSDKVKNRGDIEKVTPAPILGEVGHSEDERTLVVTKNSRRFITEQFRIIRTNLQYIV
                     GKNERPVILVTSSFSGEGKSFVSTNIGAVMALAGKKTVVMEFDIRKPKIVSGLDLKRK
                     MGITNYIIGAAGFSDLLVKVDDIDNYYVIPCGPIPPNPAELLLSPRLDELIAQARREF
                     EVVILDTAPVGLVSDAIILSKYADCTLYIVRRGRTPKNLLSVVKDLYKQKKLPSISIL
                     LNDVKQESNYYGGYYGGYGYYAYGYNNESGYFDKEGRAGRRKGFVAQIFGRFKRLFS"
     gene            1553..2404
                     /locus_tag="VKQ52_07280"
     CDS             1553..2404
                     /locus_tag="VKQ52_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87022.1"
                     /translation="MKKTDWDLVIKPKTGFFDLHLEDIWHYRDLLILFVKRDFVAQYK
                     QTILGPLWNIIQPIFTTIMFLLVFGKIANIPTDGIKPILFYMSGITIWNYFSSCFSNT
                     STTFVTNANIFGKVYFPRLVLPLSVVISNIFRFFIQFGLLLVMMIYYQVKGEYPIHFG
                     FSWLLIPVLVVIMGGIGLGLGIIISSLTTKYRDFTVLVGFGVQLLMYATPVAYPLSYL
                     MGKSYKWIVMINPLSPITEAFRYALFGVGAFSPATLGYSALFMVVSLSVGVFMFSRIE
                     RSFMDTV"
     gene            2438..3691
                     /locus_tag="VKQ52_07285"
     CDS             2438..3691
                     /locus_tag="VKQ52_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ87023.1"
                     /translation="MTNAVIKVENISKQYRLGQVGTGTLSHDINRLWHRIRKKEDPYL
                     KIGEENDRTAGGESPYVWALQDISFDVEKGEVLGIIGRNGAGKSTLLKILSRTTTPTK
                     GNIKLKGRVASLLEVGTGFHPDLSGRDNVFLNGAILGMTKPEIKRKFDEIVSFAGVER
                     YVDTPVKRYSSGMYVRLAFAVAAHLEPEILIVDEVLAVGDMEFQKKCLGKMQDVSEKE
                     GRTILFVSHNMGAIKSLCTKGMLLEKGRLSFVGETNAAINKYLRAGLVESKGYYKNPS
                     PRLESDILEIKVLGKDSNISERFAFDEEIRIVFRLKVSQKHFDALLGFRVRDQLERNV
                     FTSQVPINKFVSRAGEHEIQVTIPSSILVPNRYEIVVGFHKVNVETIQFLEAPVQFVI
                     EETGTDFFQYAGNDYGCVFVDCDWQ"
     gene            3752..4378
                     /locus_tag="VKQ52_07290"
     CDS             3752..4378
                     /locus_tag="VKQ52_07290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLZ87024.1"
                     /translation="MIKKIARKIYRSFFSQNGSQHEESLKGGYCSIGENTVFNAHHQL
                     ELRKPLSGKVFLSVGNESLIEGRFVFEKDTGCIEIGDRVFIGGGTTFICIEGIKVDDD
                     VMFSWGCTIVDNNAHSIVSAERISDVSDWKRGIAENKTGAYKNWDVVKSAPVHIKRKA
                     WIGFNSIILKGVTIGEGAVVGAGSVVTRDVPDFAVVGGNPATIIKYTT"
     gene            4383..5252
                     /locus_tag="VKQ52_07295"
     CDS             4383..5252
                     /locus_tag="VKQ52_07295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ87025.1"
                     /translation="MNWHETIEYIRKEPEYSTLVQQAYFEEKLELNVERFRATNEFTE
                     TLRIIRELAPLAKDILEIGAGNGVACINFALLGYNVTAVEPDNSDTIGAGAIEKLIRQ
                     FRLENVSVHRQYAEEIDFKEKKFDIVYIRQAMHHAFDLKKFVRKSAEAIKPGGLLITI
                     RDHVIYDQPDKEWFLKMHPLHKFYGGENAFTADEYRAAITESGLTILKELKYYDSIIN
                     YYPVTDQEIQELMKKREQHVRGELKRKISFLSFLPGMLQLYKLKNGYLKREKYLNERE
                     IPGRMYSYIAQKS"
     gene            5249..>5451
                     /locus_tag="VKQ52_07300"
     CDS             5249..>5451
                     /locus_tag="VKQ52_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLZ87026.1"
                     /translation="MRNLLIVGSRGFIGKHMADYFAAISAYEVYGCDVVTDYAAANYF
                     QIDVANADYQEIFQQVRFDFCIN"
BASE COUNT         1504 a         1173 c         1452 g         1322 t
ORIGIN      
        1 ataaccaggc gttcaatgtg gagaaccagg cgtcgacgta tttgaataat atcgaagaaa
       61 gcgcgaaatc gctgcaggac caggaagtaa agatcaccat cgtcaactgg ctgctggatt
      121 atatcggcaa ggcggaaaat aactatcgtc ttgtgcctac cgatctcggg atcgccgagc
      181 cgaccctgct tgggcagatc gctcagtaca accagctggt gctcgaacgg gaatccaatc
      241 tgaaaacgac cccggcgcag aacccgctga tcacgcgcat cgacggcaat ctggacaagg
      301 tgcgccagaa tatgctccag gccctgcaga acgtccggac atcctatatc atcgcccggg
      361 atgccatcca gcagcggggg ggacaattga agagccagct caagagcatc cccggcaagt
      421 cgatgaggga gctgaacatc cagcgccggc ggaagatcct ggaagacctg tattccttcc
      481 tgctgcagaa gcggctggag acctctattt cctccgcttc gaccatttcc aactcgaagg
      541 tggtggagcc cgcggcaggc ggcgggttgc ccatcaagcc caaccggaac tccctctaca
      601 tgacctatat gcttatcggg ctgatcatcc cggtagcgat cattgcgatc atcgaggtca
      661 tgagcgacaa ggtgaagaac cggggagata tcgagaaggt gacgccggcg ccgatccttg
      721 gcgaagtagg acactcggag gacgaaagga ccctggtggt caccaagaac agccgccgct
      781 ttatcacgga acagttcagg atcatccgga cgaacctgca atatatcgtc ggcaagaacg
      841 aacggcccgt tatcctggtc acctcttcct ttagcggcga gggcaaatcc tttgtcagta
      901 cgaacatcgg cgcggtgatg gcccttgccg gcaagaagac cgtggtgatg gagttcgata
      961 tccggaagcc caaaatcgtt tcgggtctcg atctgaaacg aaagatgggg atcaccaact
     1021 atatcatcgg cgccgccggt tttagcgacc tgctggttaa ggtagacgat atcgataatt
     1081 actatgtcat cccctgcggc cccatcccac ccaacccggc ggagctcctg ctcagcccgc
     1141 gcctggatga gctgatcgcg caggcccgga gggagttcga ggtcgtgatc ctcgatacgg
     1201 cgcccgtagg gctggtcagc gacgccatca tcctgtcgaa gtatgcggac tgcaccctgt
     1261 atatcgtcag acgcgggcgc acgccgaaga acctgctcag tgtcgtgaaa gacctttaca
     1321 aacaaaagaa actgccgtcc atttccatcc tgttgaacga tgtgaagcag gaatccaatt
     1381 actatggagg atactatggg ggctatggtt attatgccta cggttacaat aatgaaagtg
     1441 gatatttcga taaagagggg cgcgccggca ggcgtaaagg attcgttgcc caaatattcg
     1501 gcagatttaa acggctcttc agctaatgga cataaaagat atggactcga atttgaaaaa
     1561 aacagattgg gacctggtta ttaaacccaa gaccggcttt tttgatcttc acctggaaga
     1621 tatctggcat tatcgggatc tgttgatact attcgtaaag agggattttg ttgcacagta
     1681 taagcaaacc attcttgggc cgttgtggaa tatcatccag cctattttca caacgatcat
     1741 gtttctcctg gtattcggca agatagccaa tattccgacg gatgggataa agccaatctt
     1801 gttttacatg tcggggatta ccatatggaa ctatttttcc agctgtttta gtaatacgtc
     1861 caccacattc gtcaccaatg ccaatatatt cgggaaggtt tacttcccgc gcctggtgtt
     1921 gcctctttcg gtggtgatct ccaatatatt ccggtttttt atccagtttg gactcttatt
     1981 ggtgatgatg atctattatc aggttaaagg tgagtatccc atccacttcg gcttttcctg
     2041 gcttctcatc cccgtgctgg tagtcataat gggcggtatt ggcctcgggt tggggattat
     2101 tatctcttcc ctgacaacaa agtatcgcga ttttacggta ttggtggggt ttggcgtgca
     2161 attgctcatg tatgcgacgc ctgtggccta tcccttatcc tatctcatgg gaaagagcta
     2221 caaatggata gtcatgatca atccgttatc cccgataacg gaggccttca ggtatgcttt
     2281 attcggggtg ggagctttct cgccggctac ccttggttac agcgcattgt tcatggtggt
     2341 cagtttgtcg gttggtgtct tcatgttcag caggatcgag agatccttta tggacaccgt
     2401 ttagcgttta acagtgaatt aatttattac gttttatatg actaatgccg tcataaaagt
     2461 agaaaacata tcgaaacaat accggcttgg acaagtaggg acgggaaccc tcagccatga
     2521 tatcaatcgt ttgtggcatc gtataagaaa gaaagaggac ccttacctga agattggtga
     2581 agagaacgat cggactgcgg gtggtgaaag tccgtatgtc tgggcgttgc aggatatttc
     2641 tttcgatgtg gagaagggag aagtgttagg gatcatcgga agaaatgggg ccgggaagag
     2701 tacgttatta aaaatattaa gccggacaac aactcccacg aaaggtaata tcaagctaaa
     2761 gggccgggtt gccagtttgc tcgaggtcgg cacgggtttt catccggacc tgagcggccg
     2821 cgacaatgtc ttcctgaacg gagcgatcct gggaatgacg aagccggaga taaaaagaaa
     2881 gtttgacgaa atagtcagtt ttgccggagt tgagagatat gtcgatacgc ccgtaaaacg
     2941 gtattcttcc ggcatgtatg tccggctggc atttgctgta gcggcgcatc tggaaccgga
     3001 aatacttatc gtcgatgaag tactggccgt gggagatatg gaattccaaa agaaatgcct
     3061 gggcaagatg caggatgtgt cggaaaagga aggcaggacc atcctgttcg tcagtcacaa
     3121 catgggcgca ataaaatccc tctgcaccaa gggaatgctc ctggagaagg gaagattgag
     3181 tttcgtggga gaaacgaatg ccgctataaa caagtatctg agagcggggc tggttgaaag
     3241 caaagggtat tacaaaaatc catcaccccg tttggaatcg gatattctcg agatcaaggt
     3301 cctggggaaa gacagcaata taagtgagcg attcgcattt gacgaggaga tcaggatcgt
     3361 tttcaggctc aaagtatcgc aaaagcattt cgatgccctg ctcggatttc gagtcaggga
     3421 ccaactggaa agaaatgtat tcacttccca ggtgccgata aacaagtttg tcagcagggc
     3481 gggagagcat gagatccagg tgacaatacc ctcttccatt cttgtcccca accggtatga
     3541 gatcgtggtg gggttccata aggtgaatgt agagaccatt caatttctcg aagcaccagt
     3601 ccaatttgtc atcgaggaaa cggggaccga tttctttcaa tatgccggaa atgattacgg
     3661 atgtgtcttt gttgattgcg actggcaatg accgatcaaa aacagggata tctttcaact
     3721 acggataagt gatagcgaga ttgccattta aatgataaaa aagatcgcca ggaaaattta
     3781 tcgttccttt ttcagtcaaa atggaagcca acacgaagag tcgttgaaag gcggatattg
     3841 ttcaatcgga gaaaatactg ttttcaatgc ccatcatcag ttggaactaa gaaagccgtt
     3901 atcgggcaag gtctttttgt ctgtcggtaa tgagagcctg atcgaaggga ggtttgtatt
     3961 tgaaaaggat acaggatgta tagagatcgg ggacagggtt tttataggag gaggtacaac
     4021 attcatctgt atcgagggta taaaggtaga cgatgatgta atgttttcct ggggttgtac
     4081 catcgttgac aataatgcgc attcgatcgt ttcggcagaa agaatatccg atgtatcaga
     4141 ctggaaaagg ggcatcgctg agaataagac cggtgcgtac aagaattggg atgttgtaaa
     4201 aagcgcaccg gtgcatataa aaaggaaggc ctggatcggg tttaactcga taattctaaa
     4261 gggcgtcaca attggagagg gagccgttgt cggtgcagga agtgtagtta cccgcgatgt
     4321 acccgatttc gccgtggttg gcgggaaccc tgcaacaata ataaaatata ctacgtaaca
     4381 atatgaactg gcacgagacc attgaatata tacgtaagga accggagtac tccacactcg
     4441 tacaacaagc ctattttgaa gaaaaactgg agcttaatgt ggaaaggttc agagcaacaa
     4501 atgaatttac cgagaccctc aggataatca gggagttggc gccattggca aaggatattt
     4561 tggaaatagg agcgggcaac ggagtagctt gtataaactt tgccttatta gggtacaatg
     4621 taaccgccgt agaaccggac aatagtgata ctatcggagc cggagcgata gaaaagctga
     4681 tccgacagtt tcggcttgaa aatgtttccg ttcaccggca atatgccgag gagatcgatt
     4741 ttaaggaaaa aaaattcgac atagtgtata tcaggcaggc catgcaccac gcatttgacc
     4801 tgaagaaatt cgtacggaag tctgccgaag ctattaagcc gggagggttg ctgatcacta
     4861 tcagggatca cgtgatctat gaccaaccag acaaggaatg gtttctgaag atgcatcccc
     4921 tgcataagtt ttatgggggt gagaatgcat ttaccgccga cgagtacaga gcggcaatta
     4981 cggagtccgg gcttacgata ttgaaagagc tgaagtacta tgatagtata ataaactact
     5041 atcccgtaac ggaccaggag atacaggagt tgatgaaaaa gagagaacag cacgtaaggg
     5101 gcgagttgaa gagaaagata tcttttcttt cttttctccc cgggatgtta caactatata
     5161 aattgaagaa tggctatctg aagagggaga aatatttgaa tgaaagagaa atacccggca
     5221 ggatgtactc gtatatagct cagaaatcat gagaaacctg ttgatcgtag gctcccgcgg
     5281 ctttatcggg aaacatatgg ccgactactt cgcagcaatc tcagcgtatg aggtgtatgg
     5341 ctgtgatgtc gttaccgact atgcggcggc caattatttc cagatcgatg tcgccaatgc
     5401 cgattaccag gagatatttc agcaggtgcg attcgatttt tgcatcaact g
//
LOCUS       DATGFG010000386         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_594213, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000386 DATGFG010000000
VERSION     DATGFG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594213"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..626
                     /locus_tag="VKQ52_07305"
     CDS             102..626
                     /locus_tag="VKQ52_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLZ87027.1"
                     /translation="MESLGKMLAPDTLRFERTLRGPIERVWAYLTESEKRGKWLAKGE
                     MELFEGGKVNLYFLHNELSPEAWDIPEKFKGMEGGHGFTGTVLRVNPPHLLSFTWEGD
                     SEVTFELHETDDAVLLQLTHRKLPTDQASRLSVLGGWHTHLDILVANFSEVVPPNFWQ
                     RYTKMEEVYRKISA"
     gene            682..>2117
                     /locus_tag="VKQ52_07310"
     CDS             682..>2117
                     /locus_tag="VKQ52_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLZ87028.1"
                     /translation="MSTQPTSIGHFAPIGDLQLYYELWGSGQPLVLLHGGGSTIESTY
                     GRILPLFAKTHTVIAVELQAHGRTRDIDRPLSFEHDADDVAALLGQLGIAKADFMGFS
                     NGGTTCLQMAIRHPELVNKLVLASAIYRRDGMPPGFFEGFPHARLEHMPGLLQEAFLD
                     VNPDPDGLQVMFDRDVTRMMAFRDIRDADIKAIKAPALVLNGDAEVVRAEHAMALSRV
                     LPHAKLAILPGGHGDYIGEICSPYKNNPLPALVTAMIGAFLRGFFCFAILALSCAGVW
                     AQTGDSVRLSSLPRPLFWENAPVRFEAGKDHLLIVAGEKTDMFRDPNVAYNTDNAPKL
                     LFMADSNFVLTAAIHHAFASKWDGGAIVLKADSLNWIKFCFEKDYTGQKRVVSVVTRG
                     ISDDCNSAPVVSNTVYYKIAKAGNVITLYYSVEGRHWYLIRHLQFDSTAPLKLGFLAQ
                     SPTGQRCTVVFSAIHYSTKKIGDPYTGE"
BASE COUNT          470 a          560 c          615 g          472 t
ORIGIN      
        1 cttgcgtgac ctgctgaacg agccggagga ttaggccgcg gccgcgaaga accggaggaa
       61 taagatccac ctcatttctg taaacacttt aaacattgta tatggaatcc ctgggaaaaa
      121 tgttagcgcc ggatacgctg cgtttcgaac ggacgctgcg cggacctatt gaaagagtat
      181 gggcctatct tacggagtcg gaaaaacgtg gcaaatggct ggcgaaagga gagatggagc
      241 tcttcgaagg cggaaaggta aacctgtatt tcctgcacaa cgagctaagc ccggaggctt
      301 gggacatacc ggaaaaattc aagggtatgg aaggggggca tggttttacc ggcaccgtat
      361 taagggtaaa cccgcctcat ctgttgtcct tcacctggga aggcgattcg gaggtcacct
      421 tcgagttgca cgagacggat gatgccgtgt tgttgcagct cacccatcgg aaattaccaa
      481 cggaccaggc ttcccgccta agcgtgctgg ggggctggca tacgcatctc gatatccttg
      541 tcgcgaattt tagcgaggtc gttccgccca atttctggca gcggtatacg aagatggaag
      601 aggtttaccg gaaaatatcc gcctaaccct tgttggagac cgtttctatg ggggtagaaa
      661 atagatcgct atttttgggg tatgagcaca caacctacct ccatcggcca ctttgcacct
      721 ataggtgatc tacagctgta ttacgagctt tggggcagcg gccaaccgct ggtgttgctg
      781 cacggcggcg gctcgaccat cgaatccacc tatggccgga ttttgccatt attcgcgaag
      841 acgcataccg tgatcgccgt ggaactacag gcgcatgggc gtacccggga tatcgaccgg
      901 ccgttgagct tcgaacacga cgccgatgat gtagccgccc tgctggggca gctcgggatc
      961 gcaaaagccg atttcatggg gtttagtaac ggcggcacca cctgtttgca gatggcgatc
     1021 cgtcatccgg agctggtgaa caaactggtg ctggcttccg cgatttatcg ccgggatggg
     1081 atgccgcccg gcttctttga aggcttcccg catgcgcggc tggaacacat gcccgggttg
     1141 ttacaggaag cctttctcga cgtcaacccg gaccccgatg gactacaggt catgttcgac
     1201 cgcgatgtca cccggatgat ggccttcagg gatatccggg atgccgacat aaaagccatc
     1261 aaggcgccgg cgctcgtcct caacggggat gcggaagtcg ttcgtgccga acacgccatg
     1321 gccctctccc gggtgctacc ccacgcgaag ctggccatcc tgccgggcgg acatggagac
     1381 tatatcggcg agatctgttc accgtataag aacaatccac tgccggcgct ggtgacagcc
     1441 atgatcgggg cctttctccg gggctttttt tgcttcgcca tcctggcgct ttcgtgtgcc
     1501 ggtgtatggg cgcagacagg ggactccgtc aggctatcct ccctcccgcg accgctcttc
     1561 tgggaaaatg cacctgttcg cttcgaggcc ggcaaggatc atctccttat cgtagcgggg
     1621 gaaaagacgg atatgttccg tgacccgaat gtagcttaca ataccgacaa cgcgcccaag
     1681 ctgttgttta tggcagacag taattttgtc cttacggccg ccatccatca tgctttcgcc
     1741 agtaaatggg atggcggggc catcgtgttg aaggcggaca gcctcaactg gataaaattt
     1801 tgttttgaaa aggattatac aggtcaaaaa agggtggtga gcgtcgttac caggggaatc
     1861 tcggacgact gcaattcggc cccggttgtc agcaataccg tctattataa gatcgccaaa
     1921 gcggggaacg tgatcacgct ttattattcg gttgagggcc ggcactggta cctgatccgg
     1981 catctgcaat tcgacagcac cgcaccgctg aagctgggat tcctggcgca atccccgacg
     2041 ggtcagcgtt gcaccgtagt attctcggct attcattatt cgacaaagaa gatcggcgac
     2101 ccgtatacgg gagaatg
//
LOCUS       DATGFG010000387         5196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_300758, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000387 DATGFG010000000
VERSION     DATGFG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300758"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1916
                     /locus_tag="VKQ52_07315"
     CDS             <1..1916
                     /locus_tag="VKQ52_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HLZ87029.1"
                     /translation="KPPIMPTATKTAKKTVRKTAVKKAAKKVAKKAANKATKKPAGKP
                     AKKPFSTNGNGSPITTGTKPTNTTPPDFIVTGQNAAALFTLKVYRGEGMALLAMNWKQ
                     GTPTPDFVGFAIEYQEPGGNQFYPVSNRLSFLDNKGNVNPNILSSRLSPIQKFRWIHF
                     PFNANQPGKFVYRVTPVFMKAGGQLSYGDFQEAAIELMAETYPGELNVAFTRGFIASQ
                     AFVDHFGTNGGVGTILPASANQGLDFKATDPNAEKALAWMGFEARQAILSVLDQAIAD
                     PKAQVRVVAYDFNDPEIVNRLVKLGSRLKIIIDDSGTHKPATACESRAAAMLAKSAGA
                     GNVLRQHMGSLQHNKTIAVDGQKIKFAIGGSTNLSWRGIYVQNNNAVILQGATAVQLF
                     FDAFDNYWSNSNNVAGFGKTASPDWNDLQLSGVDARISFSPHSATNAVLQSIAKDIAS
                     TSSSLLYSLAFLYQTKGPIRDSITTVTGNNKLFVYGLSDKSVGGLDIQRPDGNPPVGF
                     PAALLTNVPPPFKQEATGGSGIRLHHKFVVIDFDKPTARTYLGSFNFSGVADTENGEN
                     LLLIKDRRVAVSYMIEAVSMFDHYEFRDAQAKAGKAGKLFLQTPPADAKTKPWWDEDF
                     TNPAKARDRLIFA"
     gene            complement(1989..3266)
                     /locus_tag="VKQ52_07320"
     CDS             complement(1989..3266)
                     /locus_tag="VKQ52_07320"
                     /inference="COORDINATES: protein motif:HMM:NF042839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6786 family protein"
                     /protein_id="HLZ87030.1"
                     /translation="MNIRLLAAITLTTMLASCNSNPPAGSAADLPAGSFAYDLAFLQR
                     HDSVIILRDGDARVVVSPKYQGKVFTSTATGDAGPSFGWIHYKAFDGPADPHMNAYGG
                     ENRLWLGPEGGKFSLFFPPGAKMEFANWKTPAAFDTEPWTLVAADEHGVALEKQLSLT
                     NYAGTPLSIGIGRVISILDRAAIDTTLSLAADTSIHAVGYRTVNTLTNIGANAWTEST
                     GMPCLWLLDMFNPSPATTIVIPYGGGAKTGGPKNGGGVKPGGDTTKPATTDYFGEIAA
                     DRISYADNILFFKADGKSRGKLGIHPRRAKPVAGAYDAQHHVLTITLFDTDIAARYLN
                     QEWNTTKPPFSGDAVNAYNDGPLADGSQMGPFFELESVSPAAFLAPKQSQTHTHAVFH
                     FTGPEASLDAISRKVLGVSLEKIKGVFAGGGQR"
     gene            complement(3481..3759)
                     /locus_tag="VKQ52_07325"
     CDS             complement(3481..3759)
                     /locus_tag="VKQ52_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87031.1"
                     /translation="MNRDPRYRSIRSMVNDGAIKSLNEIFNHIPKSIVAADCGKKVTR
                     FTFLMENVDEFKMRELLKIGALCDLTVAQTLELAKEQYLKTHSDKLKL"
     gene            4046..4462
                     /locus_tag="VKQ52_07330"
     CDS             4046..4462
                     /locus_tag="VKQ52_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HLZ87032.1"
                     /translation="MKKQLVFTLTAMFAVMGLCAQSRNYKVVFDLSSKDTVNQQSITR
                     EIGLITDANPDAKIEVVIYGQGLDLAVKGRSRQEAAVQQLITGNKASFKVCAMTLKRN
                     NIDKSELVPGVEVVPDGIYEIISRQQEGWGYIKVGH"
     gene            complement(4523..4891)
                     /locus_tag="VKQ52_07335"
     CDS             complement(4523..4891)
                     /locus_tag="VKQ52_07335"
                     /inference="COORDINATES: protein motif:HMM:NF008740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HLZ87033.1"
                     /translation="MNLIGNLIWLICGGLLAALGYVVGGLVLCVTIVGIPFGLQCFKL
                     AGLELAPFGRRVVPTNSSMGCLAIIFNIIWLICGGFYTALVHVVFALLLAITIIGIPF
                     ARQHLKLAELSLAPFGRQVI"
     gene            complement(4910..>5196)
                     /locus_tag="VKQ52_07340"
     CDS             complement(4910..>5196)
                     /locus_tag="VKQ52_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLZ87034.1"
                     /translation="LLWRKPHFFAAAFPICGAGDPAKVGTYARKFPIWVFHGGADPVV
                     PVANSHLMVSALQAARAKVKYTEYPGVGHDSWKKAFAEPELLPWLFENHH"
BASE COUNT         1185 a         1400 c         1511 g         1100 t
ORIGIN      
        1 tcaaaccccc gatcatgcct actgccacta agaccgctaa aaagactgtc cgcaaaacgg
       61 cggtcaaaaa agccgcgaag aaagtcgcga agaaagccgc aaataaagct acgaagaagc
      121 ccgcgggaaa gcctgcgaag aagcccttct ccactaacgg caacggctct cccatcacca
      181 ccggcaccaa acccaccaac accaccccac cggattttat cgtcaccggg caaaacgccg
      241 ccgcgttgtt cacgctaaaa gtttatcgcg gcgaaggcat ggccttgttg gcgatgaact
      301 ggaaacaagg cacccccacg ccggacttcg tcggattcgc catcgagtac caggagccgg
      361 gcgggaacca attctatccg gtgtcgaacc ggttgtcatt cctggataac aaaggaaacg
      421 tcaatccgaa tattttgtct tcgcggctgt cgccgatcca gaagttcaga tggatccatt
      481 ttccttttaa tgcgaaccag cccggcaaat tcgtctaccg cgtaacgccg gtattcatga
      541 aggcgggcgg gcagctcagc tatggagatt tccaggaggc ggcgatcgag ctgatggcgg
      601 agacctaccc cggtgaactg aacgttgcct ttacgcgggg gttcatcgcc tcgcaggctt
      661 ttgtcgatca tttcggaact aatggcggcg taggcacgat cctccctgct tcggcaaacc
      721 agggtctcga tttcaaggct accgatccga atgcggaaaa ggcattggcc tggatgggct
      781 tcgaagcgcg gcaggccatc ctcagcgtgt tggatcaggc catcgccgat ccgaaggcgc
      841 aggtgagagt ggtggcgtat gatttcaacg atcccgagat cgtgaacagg ctggtcaagc
      901 tgggcagccg gctgaagatc atcatcgacg attccgggac gcacaagccc gctaccgcct
      961 gtgagagccg ggccgcagcg atgctggcta aatcggcggg cgccggcaat gtcctgcggc
     1021 agcatatggg gagcctgcaa cacaacaaga cgatcgcggt agatgggcag aagataaaat
     1081 tcgcgatcgg aggctcgacc aacctgtcct ggcgcggcat ctacgtgcag aacaacaatg
     1141 cggtgatcct gcaaggagct acggccgtgc aattgttctt cgacgcgttt gataattact
     1201 ggagcaatag caacaatgtg gcgggtttcg gcaagaccgc gtcccccgac tggaatgatc
     1261 tccagctttc gggtgtcgat gcgaggatca gtttctcgcc gcatagcgca accaatgccg
     1321 tcctgcaaag cattgcaaag gatatcgcat cgacatcgtc cagcctgctt tattcgcttg
     1381 cttttttgta ccagaccaag ggaccgattc gagattccat caccacagta acgggcaaca
     1441 ataaactctt cgtgtacggg ctctcggaca agtctgttgg cggtctcgat atccaacgcc
     1501 ccgatgggaa ccccccggtc ggttttcccg cggccctgtt gacaaatgtg cccccgccct
     1561 tcaagcagga ggccaccggc ggatcgggta tccggctgca tcataagttc gtcgtcatcg
     1621 atttcgacaa gcccacggcg aggacttatc tcggttcgtt caatttttcc ggggtcgccg
     1681 acaccgagaa tggggagaat cttttattga tcaaggatcg ccgggttgcg gtgagctata
     1741 tgatcgaggc cgtgtcgatg ttcgatcact acgagtttcg cgatgcgcag gcgaaggctg
     1801 gtaaggcggg aaaactgttc ctgcaaacgc cgccggcgga tgcgaagacc aagccctggt
     1861 gggatgagga ttttaccaat ccggcgaagg cgagggacag gttgatcttt gcttaggtgg
     1921 tgctggcgga gaatgtggcc cattgctggc cgacggagac catggccgat ggctggttgc
     1981 cgggttggct accgctggcc gccgccggca aatacgccct ttatcttttc aagcgacacc
     2041 cccagcacct tgcggctgat cgcatcgaga ctggcttccg gaccggtgaa atgaaacacc
     2101 gcatgcgtat gcgtctgcga ttgcttcggc gccaggaacg ccgcgggcga aacactttcc
     2161 aattcaaaga agggtcccat ctggctgccg tctgccagcg gtccgtcatt atacgcgttg
     2221 accgcatctc cgctaaaagg aggctttgtg gtattccatt cctggttgag atagcgggca
     2281 gcgatatcgg tgtcgaaaag cgtaatggtc aggacatggt gctgcgcgtc ataggcgccg
     2341 gcgacgggtt tggcccgccg gggatggatg cccagtttgc cgcggctctt gccgtcggct
     2401 ttaaagaaga ggatattgtc ggcgtagctg atcctatcgg cagcgatctc tccaaaataa
     2461 tccgttgtag caggctttgt cgtgtcgccg ccaggcttca cgccgccgcc attcttcggg
     2521 ccgccggtct tcgcgccgcc accgtagggg atgacgatcg tcgtagccgg cgaggggttg
     2581 aacatatcga gcaaccagag gcagggcatg ccggtggatt cggtccaggc gttggcgccg
     2641 atattcgtca gggtattgac cgtgcgatag ccgacggcat ggatggaggt gtccgcggcc
     2701 agggatagcg tggtgtcgat ggcagcccgg tcgagaatgc tgatgacgcg gccgatgccg
     2761 atagaaagag gcgtgccggc atagttggtc agcgatagct gtttttccaa cgccacgccg
     2821 tgctcgtcgg cagccacgag tgtccagggc tccgtgtcga aggctgcggg cgtcttccaa
     2881 ttagcaaact ccatctttgc gccgggtggg aagaacagcg agaactttcc cccttcgggg
     2941 cccagccaga ggcgattctc gccgccatag gcgttcatat gcgggtcggc agggccgtcg
     3001 aaggctttgt agtggatcca gccaaagctg ggtccggcgt caccggtggc ggtggaggta
     3061 aagaccttgc cctggtattt cggcgagact acgacgcggg catcgccgtc gcggaggatg
     3121 atcacgctgt catggcgctg aagaaacgcc aggtcatagg cgaagctgcc ggcggggagg
     3181 tcggcggcgc tcccggcggg tgggttgctg ttacaggatg cgagcatggt ggtaagggtg
     3241 atggcggcaa gcaatcggat attcatagac tgaagatacc aaaaatcaaa aaaggatgga
     3301 aaaccctttg ccggggctac tacataaaaa aaaccgtccc cgggcgaaga gacggctggt
     3361 gcgaaggctg tttgggtcct ggccggccag gttgccgggg tcaacaggaa aggcaaacgg
     3421 caatggttat ggtactcagg agggcgaact atattggctg cgaactacaa ttgctgccaa
     3481 ctataattta agtttatcgg aatgggtctt caggtattgt tctttcgcca gttcaagcgt
     3541 ctgtgcgacg gtgaggtcgc agagcgcgcc gatcttcagc agttctctca tcttgaattc
     3601 atcaacgttc tccatcagga aagtaaaccg ggtcactttc ttcccgcagt ccgcggcgac
     3661 gatcgatttg ggaatatggt tgaatatctc attcagtgac ttgatggctc catcattgac
     3721 cattgatcgg atggacctgt aacgaggatc acggttcata cgcaaagaaa ggatttaaat
     3781 taactctata tacgtccaca ccatcccatt tttcctgccg cttattccct ttttttgagt
     3841 cattcctacc ctttttttag gcctcttaat ccttttccag cgcgggttcc gtaagattgc
     3901 ggacggacaa cgaatttact aaattattta gtattcaaca actattattt ttggcccggc
     3961 aagccctttg ccgctattat aaataagcat aaatttatat gtgatttcag cctgcccatt
     4021 atcaaacgat taaaactccc caggtatgaa aaaacagttg gtcttcacgc tcaccgcgat
     4081 gttcgcggtc atgggtcttt gtgcccaatc caggaattac aaagtggtat tcgacctgtc
     4141 cagtaaggac acggtgaatc agcaatcgat cacccgggag atcgggctta tcacggatgc
     4201 gaatcccgat gcaaagatag aggtggtgat ctatggccag ggtctggatc tcgctgtcaa
     4261 ggggcggagc aggcaggagg ccgcggtgca gcagttgatc accggcaaca aagcgagttt
     4321 caaggtctgc gccatgaccc tgaagcgaaa caacatcgac aaaagcgaat tggtgccggg
     4381 cgtggaggtc gtgccggatg ggatctacga gatcatttcg agacagcagg agggatgggg
     4441 ctatatcaag gtggggcatt gatcttcctg caagcatgaa tcttccacca agcatcgcat
     4501 gccatctctt cggcaaccgc cattatatca cctgtctgcc aaatggcgcc agggacagct
     4561 ccgccagttt gagatgctga cgcgcaaagg ggatgccgat gatcgtgatc gccagcagga
     4621 gcgcaaacac gacatgcacc agggccgtgt aaaagcctcc gcagatgagc cagatgatat
     4681 tgaaaattat cgccagacag cccatgctgc tattggtggg cactaccctc cggccaaagg
     4741 gcgccagctc cagaccggcg agtttgaagc attgcagccc gaatgggatg cccacaatgg
     4801 tcacgcacag caccaggccg ccgacgacgt atccgagcgc ggctagcagg ccgccgcaga
     4861 tcagccagat caaattacct attagattca tagttatgca atatagccct caatgatgat
     4921 tttcaaaaag ccagggcaat agttccggtt cggcaaaggc ttttttccag ctgtcatgcc
     4981 ctacgccggg gtattcggta tacttcacct tcgccctggc cgcctgcaag gcgctgacca
     5041 ttaaatgcga gttggccacc ggcacgacgg ggtccgcgcc gccatgaaat acccagatag
     5101 ggaatttgcg ggcatacgtg ccaaccttcg caggatcacc ggcgccgcag atggggaagg
     5161 ccgcggcgaa gaaatgcggt ttgcgccaga gcaact
//
LOCUS       DATGFG010000388         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1572829, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000388 DATGFG010000000
VERSION     DATGFG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572829"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VKQ52_07345"
     CDS             complement(<1..593)
                     /locus_tag="VKQ52_07345"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HLZ87035.1"
                     /translation="MCRSLFLWLIGSFLMLTTQAQKTIIKDPNAEVRQAKGFHGIEVS
                     SAINLYLSQGEEETVVVSASDLQWRERIRTEVVDGILKISLDTHKWSRGNVKIKAYVS
                     FTTLDKLSASGASDVFVDGVITGDHLSVDLSGASDFKGAINVKQLRLEQSGASDAHVT
                     GVVSELAEFHSSGASDVKGYDLTVQSCEVHASGASDIR"
     gene            complement(640..1362)
                     /locus_tag="VKQ52_07350"
     CDS             complement(640..1362)
                     /locus_tag="VKQ52_07350"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HLZ87036.1"
                     /translation="MKNTLLLSLFTLATLFSFAQDGKVINDRNAQKRNVQGFHGIRIS
                     SGIDLYLSQSSEEAVAVSAAEPQYRDRIVTEVDNGILHIYMENHGYHWWDRMRKHLKA
                     YVSCKALDQLTASGGSDVYVQDAIHGDRLKMDLSGGSDLHGKFTVGDLTITQSGGSDS
                     YISGSAGSLSIHTSGGSDYHGYDLAADHCQVDASGGSDAYLTVNKELTAHASGGSDIH
                     YKGNGIIRDSHTSGSGSISHRD"
     regulatory      1506..1610
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1668..3923
                     /locus_tag="VKQ52_07355"
     CDS             1668..3923
                     /locus_tag="VKQ52_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ87037.1"
                     /translation="MKKTLALFCLLAAASTLYAQTLAQPRAKDSVKLFLQPVEVRALR
                     AGEKAPFTKTDLTKQQIEALNVGQDLPFLLNQTPSVVINSDAGNGVGYTGIYIRGTDD
                     SRINMTLNGIPYNDAEEQAIFFVDLPDFASSANSIQIQRGVGTSSNGAGAFGATINFS
                     TNEFNEKPYAEFNNSFGSFDTWKNTLKAGSGLIDGHFTVDMRLSRIASNGYIDRASSD
                     LRSLYFSAAWYDKNSSIRFNILQGREKTYQAWNGIPQAKWDGNLPALQQHYQDNIGSL
                     YFNQQDSLNLFHSSNKTYNYFTYKDQTDNYWQNHYQLFFNHQFSDLLSFNSAVFLTRG
                     YGYFQEYHDQGDSANARYAAYGLPPYVVGNDTLQSTNLIRQRWLDNYFYGSVFSLQYK
                     NNNTQLMLGGGWDRYDGKHYGNVIWAANGGFPDDYRYYSEPAIKIDYNVYAKWQQQLG
                     QRWTAFADLQWRHLDYELDGFDDNPSILVHPRYNFVNPKAGITYTHYDWQAYFSYAMS
                     GHEPNRDDFEAGVSDQPTPERLHDFELGVQKKNLRYSWGATAYYMLYHNQLVLTGKIN
                     DVGDYTRSNIPHSYRLGIELQGSAKPVNWFSVDANLALSRNKVFNYVEYDDNYDNGGQ
                     VSHAYKTTDIAFSPTVVGAATLNFIPVPHLTLSLPAKYVSQEYLDNAQQDNRKLPGYY
                     VQNFRAVYTLLNKLGRQTDFIFQLNNVFNKQYTPNGYTYSYVYGGQLVTENFVFPMAG
                     TNFMVAVNIKL"
     gene            3932..>4271
                     /locus_tag="VKQ52_07360"
     CDS             3932..>4271
                     /locus_tag="VKQ52_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87038.1"
                     /translation="MLKSFLFSLGVVGVGMTLHAQTPVKRPIRPADIYRLPVVSDPQV
                     SPDGKWVSYALTTIDSLKDSRNADIWMVSWDGSEDIQLTSSPDGESRARWSPDGKWLS
                     FLSSRSPGGSS"
BASE COUNT          958 a         1146 c         1186 g          981 t
ORIGIN      
        1 cggatatcgg aggcgcccga ggcgtgcacc tcacagcttt ggaccgttag gtcatagcct
       61 tttacgtcac tcgcaccgga ggaatggaac tccgccagtt cggatacgac gccggtgacg
      121 tgggcatcgg aggcgccgct ctgctccagc cggagttgtt ttacatttat agcccccttg
      181 aaatcgcttg ccccgctgag gtcgacggac aggtgatcac ccgtgatgac cccgtcgaca
      241 aagacatcgc tggcgccgga agcgctcagc ttatcgaggg tagtgaagga gacataggcc
      301 tttatcttta catttccccg ggaccattta tgggtgtcga gactgatctt caggatacca
      361 tccaccacct ccgtccggat acgctcccgc cattgcaggt cgctggcgct gacgaccacc
      421 gtttcttctt ccccctggct caggtagaga ttgatcgcgc tggatacttc gatgccgtgg
      481 aagccttttg cctggcgaac ttcagcattg ggatccttga tgatggtctt ttgcgcctgt
      541 gtggtcagca tcaaaaaact ccctatcaac cataagaaaa gcgatctgca catgggtgtc
      601 gtttgtttgt cccattggac aagcgaatat aaggcaattt taatcgcgat gcgagatgct
      661 gcccgagccg ctggtatggg aatcacggat gatgccgttg cctttataat ggatatcgct
      721 cccaccgctg gcatgcgcgg tgagttcttt attgacggtg agataagcgt cgctgccgcc
      781 gctggcgtcg acctggcagt ggtcggcggc gagatcgtaa ccgtggtagt cgctaccgcc
      841 gctggtatgg atagagagac tgccggccga accgctgata taggagtcgg agccacccga
      901 ctgggtgatg gtcaggtcgc cgacggtgaa tttaccgtgg agatcgctgc cgccggaaag
      961 gtccatcttc aggcgatcgc cgtgaatggc gtcctggaca tagacatcgc tgcccccgga
     1021 agccgtcagt tgatccaggg ccttacagga tacgtaggcc ttgagatgtt tgcgcatccg
     1081 gtcccaccaa tggtagccgt gattttccat atagatatgg aggatcccgt tgtccacttc
     1141 ggtgacgatc cggtcgcggt attggggttc ggcggcgctt accgcaacgg cttcctcgct
     1201 actctggcta aggtagagat cgatgccact ggaaatccgg ataccatgaa aaccctgaac
     1261 attgcgtttt tgggcatttc tatcgttgat gaccttacca tcctgcgcaa aggagaacag
     1321 ggtggcaagg gtaaaaaggc tcagcagcaa agtatttttc ataatcgtag ttttttgttc
     1381 gcttaatggc ccctggatgg aagggaatgt tgcatggagg gtaggacggg cgacagggcc
     1441 caaaggttac aggtgcggtg aaaaaacccg tacatgggcg cagccaaaaa aaacctatct
     1501 ttgtggcctg ttcctcgggg tgctccctgc cttgccggca ggtggctgag aaaatacccg
     1561 cagaacctga tcagggtaat gcctgcgcag ggaaggtgat ctccacatta ttcattcctc
     1621 tcctttcgcg gcattcctgg tgttttatta gtgacaactt acgctttatg aagaagacac
     1681 tggctctgtt ctgccttttg gcggccgcct cgacccttta cgcccagacg ctggcccagc
     1741 ccagggcaaa agattcggta aaactgttcc tgcaacccgt ggaagtacgg gcgctgcggg
     1801 caggggaaaa agcccccttt accaagaccg atctcaccaa acaacagatc gaagcgctca
     1861 acgtaggcca ggacctgccg ttcctgctca atcagacccc ctcggtggtg atcaattccg
     1921 atgccggcaa cggggtcggg tacaccggga tctatatccg gggaacggac gattcccgga
     1981 tcaacatgac gttgaacggc atcccctata acgatgcgga agagcaggcc attttctttg
     2041 tcgatctgcc ggactttgcc tcctcggcca acagcatcca gatccagcgc ggggtgggta
     2101 cctcctccaa cggcgcgggg gcctttggcg ccacgatcaa tttctcgacc aatgagttca
     2161 acgaaaagcc ctatgccgag ttcaataaca gctttggcag tttcgatacc tggaagaaca
     2221 cgctaaaggc cggttcgggg ctcatcgatg ggcattttac cgtcgatatg cgcctgtccc
     2281 ggatcgccag caacgggtat atcgaccgcg cctcaagcga tctgcggtcg ctgtattttt
     2341 ccgccgcctg gtatgacaag aactcttcca tccgcttcaa tatcctgcag ggccgcgaga
     2401 agacctacca ggcctggaac ggtatccccc aggccaagtg ggatggcaat ctgcctgcgc
     2461 tgcagcagca ttaccaggac aatatagggt cgctttattt caatcagcag gattcgctga
     2521 accttttcca ttccagcaat aagacctata actatttcac ctataaggat cagacggata
     2581 attactggca gaaccactac cagctgttct tcaaccacca gttctccgac cttctctcgt
     2641 tcaacagcgc ggtctttctc acgcgcggct atggttattt ccaggagtat catgaccagg
     2701 gggattcggc gaatgccagg tatgcggctt atggcctgcc gccctatgtg gtgggtaacg
     2761 atacgctgca gagtactaac ctgattcgtc aacgctggct cgataactat ttctatggaa
     2821 gcgtgttctc cttgcagtat aagaacaaca atacgcagct catgctcggc ggtggctggg
     2881 accggtatga cggcaagcat tacggcaatg tcatatgggc ggcaaacggc gggttcccgg
     2941 acgattaccg gtactattcc gaacctgcta tcaagatcga ctataacgtt tatgccaagt
     3001 ggcagcaaca gttgggtcag cgctggacgg cctttgccga cctgcaatgg cggcatctcg
     3061 attatgaatt ggacgggttt gatgacaatc catccatcct cgtgcatccc aggtacaatt
     3121 tcgtcaatcc gaaggcgggt atcacttata cgcattatga ctggcaggcc tatttctcct
     3181 atgcaatgtc ggggcacgag cccaaccggg atgatttcga agcaggggtg agcgaccagc
     3241 caacgccgga gcggctgcac gatttcgagc tgggggtgca gaagaagaac ctgcgctatt
     3301 cctggggagc cacggcctat tatatgttgt atcacaacca gcttgtgctg acggggaaga
     3361 tcaacgatgt gggggactat acgcgtagca atatcccaca cagctaccgg ctggggatcg
     3421 aattgcaggg aagcgcaaag ccggtgaact ggttttcagt ggacgccaat ctggccctga
     3481 gccggaataa agtgttcaac tatgtcgaat acgatgataa ctacgacaat ggcgggcagg
     3541 tgagccatgc gtataagacg accgatatcg cgttctcgcc gaccgttgtc ggagccgcca
     3601 ccctgaattt tatcccggtg ccccatctga cgctgagcct gccggcaaaa tatgtcagcc
     3661 aggaatacct ggacaatgca cagcaggata accggaagct acccggctat tatgtacaga
     3721 atttccgggc cgtatacacg ctgttgaaca agctgggcag gcagaccgac tttatctttc
     3781 agctgaacaa tgtcttcaac aaacagtata ccccaaacgg ctatacctat agctatgtct
     3841 atggcgggca gctggtcacc gagaatttcg tgttcccgat ggcgggaacg aattttatgg
     3901 tggcggtgaa tattaaattg taaattggag gatgttaaaa tccttcctgt tctctctcgg
     3961 tgtggtgggc gtcggaatga cgttacacgc ccaaactcct gtcaaacggc cgatacggcc
     4021 cgcggatatc taccggcttc ctgttgtgag tgacccccag gtatcgcctg acggcaaatg
     4081 ggtgtcctat gcgctaacga ccatcgactc gctgaaggat agccgcaatg ccgatatctg
     4141 gatggtgagc tgggacggca gtgaagatat tcaactcacg agcagcccgg atggggaatc
     4201 gcgggcgcga tggagcccgg atgggaagtg gctgtccttc ctttcgtcgc gttcgcccgg
     4261 aggttcttcg g
//
LOCUS       DATGFG010000389         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1051293, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000389 DATGFG010000000
VERSION     DATGFG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051293"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VKQ52_07365"
     CDS             complement(<1..1104)
                     /locus_tag="VKQ52_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLZ87039.1"
                     /translation="MELSKNYIPAATEEKWSEHWLSRRYFHSKPDGRPAYSVVIPPPN
                     VTGVLHMGHTLNETVQDILVRKARMSGFNACWVPGSDHASIATEAKVVGMLRERGIDK
                     NQLSRPEFMEYAYEWKDKYGGIIYEQIRRLGCSVDWDRVSFTMDPDYYTSVIDIFIDL
                     YKKGLIYRGARMINWDPQAKTALSDEEVEYRDEQGKLYYVNYALVDGKGKPLGRSITI
                     ATQRPETIMGDTAVCVNPNDERYRDLAGAFAVIPLVNRRVPIIFDEYVDPAFGTGALK
                     VTPAHDINDYNLGLKHSLPIIDTLNEDGTLSPAAEVFVGMDRFAARKAVIARLKEEGL
                     IQKEETHLTRLGFSQRTNAVVEPRISTQWFVKMK"
     gene            complement(1614..1742)
                     /locus_tag="VKQ52_07370"
     CDS             complement(1614..1742)
                     /locus_tag="VKQ52_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87040.1"
                     /translation="MEQMDTIRAKESTAKESTVMVFITAFLYFAAILLFAYLFTMS"
     gene            1873..3243
                     /locus_tag="VKQ52_07375"
     CDS             1873..3243
                     /locus_tag="VKQ52_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HLZ87041.1"
                     /translation="MYAIVDIETTGGYAANNAITEVAIVLHDGNRELKRYETLVRPGM
                     TIPRYVQALTGISDDMVSGAPSFEEIAPIIHEWLKDAVFVAHNVNFDYSFLHSQLKDC
                     GYHLDSRKLCTVRLSRKVFPGASSYSLGNICQHLGISIPNRHRAGGDANATVLLFEKI
                     LQAGGLEVIRGMLKGRNREQYLPIHLPAEQLDRLPQVPGVYYFHDQKGKVIYVGKAKN
                     LRHRVCSHFSNNKTGRQKQEFLRNIYSISHETTGTELMAYLLECIEIKRLWPLYNRSL
                     KRFEPTYGLYTYEDRNGYSRLILEKRIKQLQPVYTFSLLLEGQNLLRKLIREFDLCPR
                     LCFVQKGEHRCEGIAEGTCKGACEQQEPPESYNQRVDAAVTALLQSLPSFTLMDRGRH
                     ADEKSCILVEQGRLYGMGYLPADVAVYDTTDLKSYLTRYPENDYMRGLIYAHAERWPA
                     KRYDLQ"
BASE COUNT          789 a          874 c          856 g          750 t
ORIGIN      
        1 tttcatcttt acgaaccatt gggtggagat ccgcggctcg acgacggcat tggtgcgttg
       61 cgagaagccc agcctcgtca gatgggtttc ctccttctga ataaggcctt cctctttcaa
      121 acgggcgatc acggccttac gcgcggcaaa gcgatccatc ccgacaaaaa cctccgcggc
      181 ggggcttagc gtgccgtcct cgtttagcgt gtcgatgatg ggtagcgagt gtttcaaccc
      241 cagattgtag tcgttgatat catgcgcagg ggtcaccttg agtgcgccgg tgccaaaggc
      301 cggatcgaca tactcgtcga agatgatggg aaccctccgg ttgacgagcg ggataaccgc
      361 aaaggcgccg gccaggtccc gataacgctc atcattcgga ttgacacaaa cagcggtatc
      421 ccccatgatg gtctccggcc gctgggtggc gatggtgatg ctcctgccca acggtttgcc
      481 cttgccgtcg acaagcgcat agttgacata atacaattta ccctgctcgt cccggtattc
      541 cacttcttcg tcactaagcg ccgtctttgc ctggggatcc cagttgatca tccgcgcgcc
      601 acggtagata agcccttttt tatagaggtc gataaagata tcgatgacgg aagtatagta
      661 atcggggtcc atggtgaacg ataccctgtc ccagtcgacg ctgcaaccca gccggcggat
      721 ctgttcatag atgatcccgc catacttatc cttccattca taggcatatt ccatgaattc
      781 cgggcgggaa agctggttct tgtcgatccc ccgctccctg agcatgccga ccacctttgc
      841 ctccgtggca atggaagcat ggtcgctgcc gggcacccag caggcattaa agccgctcat
      901 ccgcgccttc cggaccagga tatcctgaac cgtctcattc agcgtatgac ccatatgcag
      961 tacgccggta acattgggtg gcgggatgac cacgctgtag gcggggcgcc cgtcgggttt
     1021 gctgtggaaa taacgcctgc ttagccagtg ttcggaccat ttttcctctg tcgcggccgg
     1081 gatatagttc ttgctcagtt ccatgatcat gcaatgttta gctctacaaa tgggtggcaa
     1141 aggtacgaat cgggcaaaaa aaattcccca tgatggggaa ttgcttttaa gaatattttt
     1201 ggtgtatatg actcccgcct tacttaatct ggtcggcaag gcgcaatttt ttcggtagta
     1261 taaacctatg caaaccagta caggtaagac aggaacatca ccaggccgat aaaaccaatg
     1321 gccagtaaga atgtttttgc tgtttctccg agatggatgg attggaagac ctttttcata
     1381 aacaaaattt tttagggtga aaccaaatga ttaaaataaa tgggtttcaa aggggatcgg
     1441 aaattttgac gaaaaacggt ttctcagatg aatttggcgt ggaaggttgc cggtctaaag
     1501 gtcgtagaac tagtaggttc cacaaaaata acacaatttt tgagaaccgg aaattctttt
     1561 ttggtgcttc cagaccacac ctaacccatt gcttctgagg gttttcctat tgcttaagac
     1621 atcgtgaaca ggtaggcgaa caacagaatg gcggcgaaat agagaaacgc agtgataaat
     1681 accataacgg tactctcttt tgcggtgctt tctttggcgc ggatagtgtc catttgttcc
     1741 atacagattt ggatttaggt taaaaaatta aaacacgaca ggaggtatcc tgcggtcatc
     1801 catagagtaa cggaacaaat attttacctt tacagaccca cctttgcaat aggatccggg
     1861 aaggtagata gaatgtacgc gatagtcgat atagaaacta ccggcggtta tgcggctaat
     1921 aacgccataa cagaagttgc tattgtgctg cacgatggaa accgggagct aaagcgatac
     1981 gagacccttg tccggccggg tatgaccatc ccccgctatg tccaggccct caccggcatc
     2041 tccgacgaca tggtgtcggg tgctccctcc ttcgaagaga tagcgccgat catccacgag
     2101 tggctgaagg acgccgtttt tgtcgcgcat aacgtcaact tcgattattc ctttttgcat
     2161 agtcagctga aggattgcgg ctatcatctc gacagccgca aactctgcac ggtgaggctc
     2221 agccgcaagg tcttccccgg cgcttccagc tacagcctgg gaaatatctg ccagcatctc
     2281 ggcatctcga tccccaaccg gcaccgggcc ggcggcgacg ccaatgccac cgttctgctc
     2341 ttcgagaaga tcctgcaggc cggcggcctg gaggtcatcc gcgggatgtt gaagggcagg
     2401 aacagggagc aatacctccc gatccacctg cccgccgaac aactcgaccg gctgccgcag
     2461 gtgcccgggg tctattattt tcacgaccag aagggaaagg tcatctatgt cggtaaggcc
     2521 aagaacctcc ggcaccgggt ctgcagtcat ttctccaaca acaagaccgg gcggcagaaa
     2581 caggaattcc tgcggaatat ctactccatc tcccatgaga ccaccggaac ggagctgatg
     2641 gcctacctgc tggaatgtat cgagatcaaa cggctctggc cactctataa ccggagcctg
     2701 aagcggttcg agcccaccta tggcctctat acctatgagg accgtaacgg ctactcccgg
     2761 ctgatcctgg aaaaacgaat aaagcaatta cagcccgtct ataccttctc ccttctgctg
     2821 gaagggcaaa atctcctgcg aaagctgatc cgcgaattcg atctctgtcc gcggctttgt
     2881 tttgtccaga aaggcgaaca tcgctgtgag gggatcgccg agggaacctg caagggggcc
     2941 tgtgaacagc aggagccgcc ggaaagttat aatcagcggg tggatgcggc ggtcaccgcc
     3001 ctgctgcaaa gcctgcccag ctttacgctg atggatcgcg gccggcatgc cgacgagaaa
     3061 agctgtatcc tggtggaaca gggacggctg tatggtatgg gctatctccc cgccgatgtg
     3121 gccgtgtacg ataccaccga tctgaaatcc tatctcaccc gctatcccga gaacgattat
     3181 atgcgcggcc tgatctatgc ccacgccgaa cggtggcccg caaaaagata cgatctgcaa
     3241 taaataaaaa agcccgacca tcggccggg
//
LOCUS       DATGFG010000390         5342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_943485, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000390 DATGFG010000000
VERSION     DATGFG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943485"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="VKQ52_07380"
     CDS             <1..1816
                     /locus_tag="VKQ52_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87042.1"
                     /translation="EVLSWRPGSPFRREAFVSFYDYARPGMTEAVVDLNTGKPVSVKQ
                     IPNVIGMGLDADSLVAETIVRKDSGWAAALKKRGLDIEGVTHRSIFPGDLGLAPIGHR
                     EQLVIPRIKENKIDIEGLLAYVDLTAGKVLKIIDEPGRFSGRTDLNYFNEDSARDTRK
                     GPAPIHISQPEGSSVTIDGQAISWQNWRLRFGIDNREGLVIYNVRFVDNGRERPVLYK
                     GSMPEMVVPYGAPGLLQAAYNFFDAGEYRLGQGIARPMTPGSDAPENAVYLPATLHRE
                     SGVPFQLNNAVAIFEEYGGTLWRHGTVSRRATNLAIKYYTQIENYDYGFTWRFKEDGT
                     IDIDIELTGIVEVQGVHRTSAMDSPDKNDHSWNGMPFGTLVKPHVEAINHQHFFVFRL
                     DMDVDGEAGNGVMEMNARSVPVGPENQYGNAFVVEHTHFMTEQQAQRSVNMASSRCWH
                     IMNNHLHNGLGQHTGYMLMPGGQAAPFAPPGTILRKKAGFLDHQFWVTQYNDEEEYPA
                     GRYPASNKVYDGIKQWTAKNRPIGNNDVVCWYVAGITHIVRPEEWPVMPCHHLGFSLM
                     PFGFFPENPTMGQANPDFIKHLKPLSSPAQTLPGQPVN"
     gene            1935..2861
                     /locus_tag="VKQ52_07385"
     CDS             1935..2861
                     /locus_tag="VKQ52_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLZ87043.1"
                     /translation="MKSAQKLSYGCLYGTLYLLSLLPFPLLYALSDLVCFFLFQVFGY
                     RKGVVVKNLRNSFPDKSPQEIDRLAHEFYHYLCDLFLEMFKTLSISRESMLRHCRMSP
                     EALAVFNKLSEEGKSLILVCGHYGNWEWAGNTFSLVCRQHLSVIYHPLSSQAFDRLMY
                     KMRTRFGTGLIEMNSTFRTMVKQKGTLTATAFIADQTPFPERAYWTQFLNQDTPVFMG
                     PETIARKLNYPIVYTSVERERRGYYRIHAELLVEKPAATVDGEISELHTRRLEQDIVS
                     HPEIWLWSHKRWKYSRPAAHTSTSTPNVINPC"
     gene            2855..3832
                     /locus_tag="VKQ52_07390"
     CDS             2855..3832
                     /locus_tag="VKQ52_07390"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLZ87044.1"
                     /translation="MLSGKELILSTKEYASEDIRASWAHTVSTLGLLFLAHTGTLYMP
                     TLIGKAACSVFAGLIIIRSFIIYHDFQHHAILYRSRTANVVMTVFGWYILAPASIWKR
                     SHDYHHQHNSKLFSADIGSYPIVTKCKFQAMGRKQRFSYLAIRHPVTILFGYITMFIW
                     GMCMRSFLNNPKKHLDSLFAILLHLAGSVLIWLIFGFQGWLLCVFIPFFIAQALGSYL
                     FYAQHNFPGVVFKDRCGWTYEGAAMESSSYMRMGPFMQWCTGNIGYHHIHHLNCRIPF
                     YKLPRAMECIGELQQAKITSLKPKDILACLRLKVWDPERQRMIGYREIF"
     gene            complement(3838..>5342)
                     /locus_tag="VKQ52_07395"
     CDS             complement(3838..>5342)
                     /locus_tag="VKQ52_07395"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HLZ87045.1"
                     /translation="GPDRVASGSALQGAADYLDAHASVIVLIWLVCLAVQLMRLTGGL
                     YRIHRIRQSSASPAIGEWERKLVFLARRMGIRREVALLQSGFVRMPAVTGILKPVILV
                     PMGMLAQIPPEQVETIFLHELAHIRRSDYLLNLLLHLSEAVFFFNPGIRWMASLLREE
                     REACCDDAVLAATGDKNIYFDALVGFMQFAVGRQELAMPLGGGKSDLLWRVRRMLTQE
                     NKKLHIMEKTVLSFGLMALVAVGLISMRERGTRQTPKAKDSVPVAVIRPSKDTVPRKH
                     STETHFSGTYSSDGHSFSPLNININDTKDGRVLKATATDENGDVYTLKEVDDKVTEFS
                     INGKQIAQEDYSKYVDIFDRIEDAAVPPVPPVPPVPPVPPVPPVPPVPPVPPVAPVPP
                     VPPVPPVPPVPPVPPVPPVPPVPPVPDYFVRHIIRDLLNDKLIDTKDPLSFTLDKNGL
                     TVNGVKQPAEVYQRYKEKYLELPKDHFIYEHHGGSTHSEIETDKKSPEFI"
BASE COUNT         1219 a         1600 c         1368 g         1155 t
ORIGIN      
        1 agaagtcctt tcctggcgac cgggtagccc cttccgccgc gaagccttcg tcagcttcta
       61 cgactatgcg agacccggca tgacggaagc cgttgtcgat ctcaacaccg gcaaacccgt
      121 ctcggtgaaa cagatcccca acgtcatcgg catgggcctc gacgccgact cccttgtcgc
      181 ggaaaccatc gtccgtaagg attccggctg ggccgcagcg ttaaagaaac gaggcctcga
      241 tatcgaaggc gttacccacc gcagcatctt ccccggcgac cttggtctcg cacccatcgg
      301 ccatcgggaa cagctcgtaa taccccggat aaaggagaat aagatcgaca tcgagggctt
      361 actcgcctat gtcgatctta ctgccggcaa ggtcctgaaa ataatcgacg aaccgggtcg
      421 gttcagcggc cggaccgatc tcaactactt caacgaagac tcggcccgcg acacgcgaaa
      481 aggcccggcg cccatccaca tctcccaacc cgaaggcagc tctgtcacta tcgacggcca
      541 ggccatcagt tggcagaact ggcggctccg cttcggcatc gacaaccgcg aaggcctcgt
      601 catctataat gtccgcttcg tggacaatgg ccgtgaacgt cccgtgctat acaagggctc
      661 catgcccgag atggtcgtcc cttatggcgc ccccggcctt ctgcaggcgg cgtataactt
      721 cttcgacgcc ggcgaatacc gtctcggtca gggtatcgcc cgcccgatga ccccgggcag
      781 cgacgcaccc gagaacgccg tctatctgcc cgccacgctg cacagggaat cgggggtccc
      841 gttccaacta aacaacgccg tggccatttt cgaagaatat ggaggaacgc tgtggcggca
      901 tggtaccgtg tcccgccgcg ctaccaacct ggccataaaa tattacaccc agatcgagaa
      961 ctatgactat gggttcacct ggcgttttaa agaggacggg accatcgaca tcgacattga
     1021 actcaccggc atcgtcgaag tccagggcgt acaccgcaca agcgcaatgg attcccccga
     1081 caagaacgat cactcctgga acggtatgcc ctttggcaca ctggtaaagc cgcacgtcga
     1141 agccatcaac caccagcatt tcttcgtctt ccgcctggac atggatgtcg acggcgaagc
     1201 cggcaacggc gtcatggaga tgaatgcaag atcggttcct gtcgggccgg agaaccagta
     1261 tggcaacgcg tttgtcgtcg aacataccca tttcatgacc gagcagcagg cccagcgttc
     1321 ggtaaacatg gcaagctccc gttgctggca tatcatgaac aaccatctgc acaatggcct
     1381 tggtcagcac accggctata tgctcatgcc cggcggccag gccgcgccct ttgctccccc
     1441 cggtaccatc ctgcggaaga aagcgggctt cctggatcac cagttctggg tgacgcagta
     1501 caacgatgaa gaggaatatc ctgccggcag atacccggcc agcaataaag tatatgatgg
     1561 aataaaacag tggacggcaa agaacagacc catcggcaat aacgacgtcg tctgctggta
     1621 cgtggccggt atcacgcata tcgtccggcc cgaggaatgg cctgtcatgc cctgccacca
     1681 tcttggcttt tcgctgatgc ccttcggctt tttcccggag aacccgacga tgggccaggc
     1741 caaccccgac tttataaaac acctcaaacc cctttcctca cctgcccaaa ccctacccgg
     1801 ccaacctgtg aattaaaacc caaataacat attttgttgg cgtaaactcc tatttttccc
     1861 tatcttaccg gggttggggc atcctgttgc cctgttcctt ttgcaacccc tattcaagaa
     1921 gtcctacgtc agctatgaaa agcgcgcaaa aattgtcata tggttgtctt tacggtaccc
     1981 tgtacctgtt gtccctgctg ccttttcctc tcttatacgc gctttcggac cttgtctgct
     2041 ttttcctgtt ccaggtattc ggatacagga agggggtggt agtgaagaac ctgcgaaatt
     2101 cctttcccga caaatcaccc caggagatcg atcgccttgc ccacgaattt taccattatc
     2161 tctgcgacct gttcctcgaa atgttcaaga ccctgagtat ttccagggaa tccatgctcc
     2221 gtcactgccg gatgtcaccg gaagccctgg cggtattcaa taagctatcc gaagagggga
     2281 aaagcctgat cctggtctgc ggacactatg gcaactggga atgggccggt aatacgttca
     2341 gcctggtttg ccgacaacac ctttcggtga tctatcatcc cttatccagc caggccttcg
     2401 accggctgat gtacaagatg aggacccgtt tcggcaccgg tctcatcgag atgaactcca
     2461 cgttcaggac gatggtcaaa caaaaaggaa cgttgacggc caccgcgttc atcgccgacc
     2521 agacgccctt tccggagcgg gcctattgga cacagttcct caaccaggac acgccggtgt
     2581 ttatggggcc ggaaaccatt gccaggaaac tgaattatcc catcgtctat acatccgtgg
     2641 aaagagagcg aagaggctac tatcggattc atgcggagtt gctggtagag aaacccgccg
     2701 caacggtcga cggtgagata tccgaactgc acacccggag actggagcag gatatcgttt
     2761 cgcatccgga gatctggctt tggtctcata aacggtggaa atattcccga cccgctgctc
     2821 atacttcaac ctccacccca aacgttatta acccatgtta agcggcaaag agttgatcct
     2881 ttccaccaag gaatatgcat ccgaggacat ccgtgcctcc tgggcacata ctgtctctac
     2941 cctgggatta ttattcctgg cccacaccgg cacgctctat atgcccacgc tcatcgggaa
     3001 ggccgcctgc agcgtatttg ccgggttgat cattatccgg agctttatta tctaccatga
     3061 ctttcaacac catgcgattc tgtataggag caggacggca aatgtcgtga tgaccgtctt
     3121 cggttggtac atcctggccc cggccagcat ctggaagcgg agccatgact atcatcacca
     3181 gcacaactcc aaattgttca gcgcggatat cgggtcctat ccgatcgtaa ccaagtgcaa
     3241 attccaggcg atgggccgga agcaaagatt cagttacctg gccatccggc atccggtcac
     3301 catcctgttc ggctatatca ccatgttcat ctggggcatg tgcatgcggt cgttcctcaa
     3361 caatcccaaa aaacacctgg attccctgtt tgcgatcctg cttcatctgg ccggttcggt
     3421 cctgatctgg ctgatcttcg gattccaggg atggctgctt tgcgtattca ttcctttttt
     3481 tatcgcgcag gcgctggggt cctacctgtt ttatgcccag cacaattttc ccggggtggt
     3541 ttttaaagac cgttgcggct ggacgtatga gggagccgcg atggaatcct ccagctatat
     3601 gcggatggga cctttcatgc aatggtgcac gggaaatatc ggctatcacc atatccacca
     3661 tcttaattgc cggatccctt tttacaaact tccaagggcc atggagtgca tcggggagct
     3721 ccagcaggca aaaatcacct cattgaaacc aaaggatatt ctagcctgtc tccggctaaa
     3781 ggtatgggac cccgagaggc agcggatgat cgggtacagg gagatattct agccccgtta
     3841 aataaactcc ggcgactttt tgtccgtctc tatctcggaa tgcgtcgagc cgccatgatg
     3901 ttcatagatg aaatggtctt tagggagctc gaggtatttc tccttatacc gctggtacac
     3961 ctcggccggc tgcttcacgc cattgacggt gagcccgttt ttgtcaaggg taaaagataa
     4021 agggtctttt gtatcgatga gcttgtcatt cagcagatcc cggataatat gccggacaaa
     4081 ataatccggc acggggggta caggcggcac tggcggaacc ggcggtacgg gaggaacggg
     4141 cggcaccggg ggcacaggag gaacgggtgg tacaggcgca accgggggta ccggagggac
     4201 gggtggcacc ggaggaacag ggggcaccgg gggaaccggc ggcacgggag gtacgggagg
     4261 aaccgccgca tcctctatcc gatcaaagat atccacatac ttgctgtagt cttcctgcgc
     4321 aatctgctta ccgttgatgc taaactccgt caccttatca tccacttcct tcagcgtata
     4381 gacatcgccg ttctcgtcgg tggccgtagc cttcaggacg cggccatcct tcgtatcgtt
     4441 gatattgata ttcagcgggc tgaaagaatg cccatccgaa ctataggtac cggagaaatg
     4501 cgtttcggtc gaatgcttgc gggggacggt atctttcgac gggcgtataa cagccaccgg
     4561 cacactatct ttcgcctttg gtgtctgccg ggtcccacgc tcccgcatac tgatcaatcc
     4621 caccgctaca agcgccatca ggccaaacga taaaacggtc ttttccataa tatgaagttt
     4681 tttattttct tgtgtcagca tccggcgtac gcgccacaac agatcgctct tacccccgcc
     4741 cagcggcatc gccagctcct gccgtcctac ggcgaattgc ataaagccca ccagcgcatc
     4801 gaaatagata ttcttatcac ccgtggcggc cagcaccgcg tcatcacaac aggcttcccg
     4861 ttcctcccgc agcagcgaag ccatccaccg gatgccggga ttgaagaaga acaccgcttc
     4921 cgacagatgc agcagcaggt ttaacaggta gtcactgcgg cggatatgcg ccaattcatg
     4981 gaggaagatg gtctccacct gttcgggcgg tatctgcgcc agcatcccca tgggcaccag
     5041 gatcaccggt ttcaatatgc ccgtgacggc cggcatccgg acaaaccccg attgcaatag
     5101 cgccacctcc cgccggatac ccatccgcct tgccaaaaag accagttttc gctcccactc
     5161 accaatggcc ggcgaagcgc tactctgcct gatccgatgg atgcggtaaa ggccgcccgt
     5221 cagccgcatc aattgcacgg caagacagac cagccagatc agtacgataa cggaagcatg
     5281 ggcatccagg taatcggccg ccccctgcaa cgcactcccc gatgcgaccc gatcgggacc
     5341 cg
//
LOCUS       DATGFG010000391         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_871509, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000391 DATGFG010000000
VERSION     DATGFG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871509"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VKQ52_07400"
     CDS             <1..396
                     /locus_tag="VKQ52_07400"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HLZ87046.1"
                     /translation="FGPIYPFGHGLSYTSFSYSNLTITPGVQHPQGDVTVSVEVTNTG
                     SRKGDEVVQLYINDEVTSVTQYEEQLRGFQRITLAPGEKRTVQFTLRPDDLSLLDKNN
                     VWRVEPGKFQAMVGSSSTDIRLKQEFEIR"
     gene            469..>2283
                     /locus_tag="VKQ52_07405"
     CDS             469..>2283
                     /locus_tag="VKQ52_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HLZ87047.1"
                     /translation="MSPLQRSLYILIFACLLGNTLTAQRGGGGVKWGKDGNSYYALES
                     NTLVRYDLPAFQRTVVVEAKEMPIAVQGFSFSDDEKKLLLFNNTKKVWRFNTRGDYWL
                     LDLQTRALKQLGAGRPASSLMFAKLSPDGTKAAYTSEHNVYVEDLATGTITPLTTEGT
                     FRLINGTFDWAYEEEFSCRDGFRWSPDGKSIAYWQLDATKVKNFLMIDNTDSLYPFTI
                     PVEYPVAGEDPSPCRVGVVDIASRKTTWMDVPGDSRQHYIPRMEWAANSTELVLEQLN
                     RKQNDAKIFICNAATGAATPIYEETDSAWIDVKHSYSDDPTGWEWMNGGKDFLWVSEK
                     DGWRHLFRLSRDGKKETLLTKGNYDLISLKAIDEKGGYAWFMASPDNATQQYLYRVRL
                     DGKGSLERVSPSTRHGTHSYTISPNALYAMHNFSNHLYESVSEWVSLPKHATIKTNDQ
                     PNRTGGRPQYPVQLFQLTTDDGVTMDGWMILPKGFDSTKKYPVLFNVYTEPAATTVRD
                     VAGAAGTPLYAGDIAADGYIQISLDGRGTPAPKGRTWRKCIYRKIGIINIRDQAMAAK
                     KILQWPFVDTSRIAVHGWSGGGSATLNLLFQYPDIYKTG"
BASE COUNT          582 a          756 c          551 g          394 t
ORIGIN      
        1 ttcggcccca tctatccctt tggccacggc ctcagctaca cgagcttctc ctacagcaac
       61 ctcaccatca cccccggcgt tcagcacccc cagggcgacg tcacggtctc cgtcgaggta
      121 accaacacgg gcagccgaaa aggggacgag gtggtacaac tctatataaa cgacgaggtc
      181 accagcgtaa cccaatatga agaacaactc cgcggcttcc agcgcatcac cctcgcaccg
      241 ggtgaaaaac ggaccgtgca attcaccctc cgccccgacg acctgtccct cctcgacaag
      301 aacaatgtct ggcgagtaga gccgggtaaa ttccaggcaa tggtcggcag cagttccacc
      361 gacatccggc taaaacagga attcgagatc aggtaatttc cgtacataaa agatcaaaaa
      421 caaggaagcg ccgccatgcg gcgtttctta tattgggtca aattccatat gagcccgcta
      481 caaagaagcc tgtacatatt gatctttgcc tgcctgcttg gcaacaccct caccgcccag
      541 cgtggcggcg gcggtgttaa atggggtaag gacggaaaca gctactatgc cctcgaaagc
      601 aataccctcg tccgctacga cctgcccgcc ttccagcgca ccgtcgtcgt cgaggcaaaa
      661 gaaatgccga tcgccgtcca gggcttttcc tttagcgacg acgaaaaaaa gctccttctt
      721 ttcaacaaca caaaaaaggt gtggcgcttt aacacccgcg gagactattg gctgctcgac
      781 ctccagaccc gtgcactgaa acagctgggc gccggcagac ccgcttcctc cctgatgttc
      841 gccaaactct cccctgacgg aacaaaagcc gcctacacaa gcgaacacaa tgtctacgtg
      901 gaagacctcg ccaccggcac gatcacccca ctcactaccg aaggaacatt ccgcctgata
      961 aacgggacat ttgactgggc atacgaagag gaattcagct gccgcgacgg cttccgctgg
     1021 agccccgacg gcaaaagcat cgcctactgg cagctcgacg caacaaaggt gaagaacttc
     1081 ctgatgatcg ataacaccga ctcgctctat cccttcacca tccccgtgga ataccccgtg
     1141 gccggcgaag acccctcgcc ctgcagggta ggggtggtcg acatcgcctc ccgcaaaacg
     1201 acctggatgg atgtccccgg cgacagccgg caacactata tcccccgcat ggagtgggcc
     1261 gccaacagca ccgaactggt gctcgaacag ctcaaccgca aacagaatga cgcaaagata
     1321 tttatctgta acgccgccac cggtgccgcc acacccatct acgaggaaac cgatagcgca
     1381 tggatcgacg taaaacacag ctatagcgat gaccccaccg gctgggaatg gatgaacggc
     1441 gggaaggact tcctctgggt ctccgaaaaa gatggttggc gtcacctctt ccgccttagc
     1501 cgcgacggga aaaaagaaac cctcctcaca aaaggcaatt acgatctgat cagtctcaaa
     1561 gccatcgacg aaaaaggcgg ctacgcctgg tttatggcct cgccggataa cgcgacccag
     1621 caatatctct accgcgtccg cctcgacggc aaaggcagtc tcgaacgggt atccccctcc
     1681 acccggcatg gcacccatag ttacactatc tcacccaacg ccctctacgc catgcacaat
     1741 ttctccaatc atctctacga gtcggtctcc gaatgggtga gcctgccaaa acatgcgacg
     1801 ataaagacaa acgatcaacc caaccgcacc ggcggccggc cgcaataccc ggtgcagctc
     1861 ttccagctca ccaccgatga tggcgtcacg atggatggct ggatgatcct gccaaaaggc
     1921 ttcgacagta caaaaaaata tcccgttctc ttcaacgtct ataccgaacc tgccgccacc
     1981 accgtaaggg atgtggccgg cgccgcgggc acccccctgt atgccggcga catcgccgcc
     2041 gacggctata tccagataag cctcgacggc cgcggcaccc cggcgccaaa aggcaggacc
     2101 tggcgaaaat gcatctaccg gaagatcggt atcatcaata tccgcgacca ggccatggcg
     2161 gcaaaaaaga tcctccaatg gccctttgtc gacacgagcc gcatcgccgt tcacggctgg
     2221 tccggcggcg gctccgccac cctgaatctg ttgttccaat accccgatat ctataagacc
     2281 ggc
//
LOCUS       DATGFG010000392         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1160416, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000392 DATGFG010000000
VERSION     DATGFG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160416"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VKQ52_07410"
     CDS             complement(<1..256)
                     /locus_tag="VKQ52_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87048.1"
                     /translation="MLTQHNDLQRTGWNAQEKTLNTKNVKPGTFGKLYTYTVDDEVFA
                     QPLVVTGLTIGGGTHDVVYICTVNNTIYAFDADSARSAGPY"
     gene            591..2006
                     /gene="gndA"
                     /locus_tag="VKQ52_07415"
     CDS             591..2006
                     /gene="gndA"
                     /locus_tag="VKQ52_07415"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HLZ87049.1"
                     /translation="MANNSFKFGMIGLGVMGRNLLLNMADHGFAVVGFDKDAQKGATL
                     ESSASPGTTVRGVTTLAEMIQLLEKPRKMMMLVPAGKPVDDVLHDLLPLLEKGDIVID
                     GGNSHYTDTLRRVTSMEEKGFHFMGVGISGGEQGARTGPSIMPGGDPEAYKDVQPLLE
                     AVAAKVNGVPCVAHLGKGAAGHYVKMVHNGIEYAIMQLISETYDLLHRGLGLGNDELH
                     KIYKNWNDGELQSFLVEITRDIFLKQDDKTDNRLVDMILDKAGAKGTGKWTSQDAMDL
                     GVPIPVIDMAVCLRELSAYKEERTQAAALYKNPSSDIPVPREVFIQQTQDALFFGVII
                     SYAQGLAMLHIASKELDMQIPIQEVVKVWRGGCIIRSSLLEVFFKAFQKNPELPNLLL
                     EGEIAQLLQAKKQNMRNLIIHAIHMGYPVAGLMAALSYFDAYRSERMPTNLIQAQRDY
                     FGAHTYQRIDMPGVFHTEWNK"
     gene            2034..3536
                     /gene="zwf"
                     /locus_tag="VKQ52_07420"
     CDS             2034..3536
                     /gene="zwf"
                     /locus_tag="VKQ52_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLZ87050.1"
                     /translation="MQNHKRPPASLIFIFGGSGDLNYRKLSPALYNLFIDGWMPDKFG
                     IVGIGRSPYNDEDYRQRLLNGIQQFSRRKGEPNGHWDEFSQHVTYLQMDAEKEGDYQK
                     IADVVKSKETEMGEHPNVIFYLSVAPQLMPDIAQKLGALKLCSETSTTRIVIEKPFGH
                     DLKSAHELNQLLMGMFKEEQIYRIDHYLGKESVQNILSLRFANALFEPIWSRHYIDYV
                     QITAAETVGVEGRGGYYEQSGALRDMVQNHILQLMCMIAMEAPVSFDANEIRNKKVDV
                     LNAIRRISKEEVNQVAVRGQYGEGWIKGEKVPGYKQEKSVNPNSAVETFAAVKFYIDN
                     WRWQDVPFYVRTGKRMNQKTTSITIQFKAAPSYAFPPEAAESWRANRLTISVQPEMEV
                     RLRFQAKRAGQSMTLNPVDMIFNPKDNEHEPEAYETLLLDVMEGNPTLFMRADQVESA
                     WKIVMPILETWETRAPANFPNYTPDSWGPEDAEALIARDGHVWITMPPIK"
     gene            3546..4268
                     /gene="pgl"
                     /locus_tag="VKQ52_07425"
     CDS             3546..4268
                     /gene="pgl"
                     /locus_tag="VKQ52_07425"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HLZ87051.1"
                     /translation="MELHVFKDSEALSNAAAKFIADSITTTLKTKPRYTIALSGGSTP
                     KRLHQILAQSPYKEQIDWTKLHVFWGDERAVPFEDSRNNAKMAYDTLLDFVPVPASQI
                     HVMRTDIGPEQSAAEYEKILHQYFDGAATSFDLVLLGMGDDGHTLSLFPGTPVIHEEK
                     LWATAFFLKAQDMYRITLTKTIVNRSACVAFLTAGTAKANALKEVLKGAYNPDLYPSQ
                     VIKPVGELHWFVDEAAAAKISS"
     gene            complement(4252..>4498)
                     /locus_tag="VKQ52_07430"
     CDS             complement(4252..>4498)
                     /locus_tag="VKQ52_07430"
                     /inference="COORDINATES: protein motif:HMM:NF021522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HLZ87052.1"
                     /translation="VLDALHIHETDEEEEPPAPAQLKIIWARDWMKAALVIDGHVHAL
                     FDFAAHGGYNINEFPPPNDFWTQGDRKLSDEMIRKIF"
BASE COUNT         1166 a         1307 c         1137 g          888 t
ORIGIN      
        1 agtaaggtcc ggccgaccgc gcactgtcgg cgtcaaacgc atagatcgta ttattcaccg
       61 tacagatata gaccacatcg tgtgtcccac caccgatcgt cagaccggtg acgacaagcg
      121 gctgcgcaaa tacttcgtca tccacggtgt aggtgtacag cttcccgaag gtccccggct
      181 tgacgttctt ggtgttgagg gttttctcct gcgcattcca gcccgtccgt tggagatcat
      241 tgtgctgcgt cagcatgtca acctgagagt aaagggtctg acaaacaaca aacatgagag
      301 cacagaatag aataaagctt ctcatcctga aggttgattt aaggtaaaaa aggaaccgag
      361 cctgtcacgg ttggatcata gtgacataag agattgaagc ggccttacat gggaaattcg
      421 aagaaaaatg aagcgagtga gtatacaaaa atacaaaagg atttcatata tcctaacatt
      481 taaggccctt ttatgggtgt tcaacgcccg ccgtaatcca agcttaatgc gaaagggatg
      541 tatttcaatg ataaaatgcc taaatttatg ccctatatta acattaattc atggcaaaca
      601 attcatttaa attcggcatg ataggcctcg gtgtgatggg gcgcaaccta ctgctaaata
      661 tggccgatca cggctttgca gtagtcggct ttgataagga cgcgcaaaaa ggagcaaccc
      721 tcgaatcatc cgcttccccc ggtacgacgg tcaggggcgt gaccacgctt gccgaaatga
      781 tccagctgct cgaaaaacca agaaagatga tgatgctggt ccccgcaggc aaaccggtgg
      841 acgacgttct tcacgatctt ctcccgctgc tggaaaaagg cgatatcgtc atcgacggcg
      901 gcaactccca ctatacagat actctccgtc gcgtaacctc catggaagag aaaggcttcc
      961 acttcatggg cgtcggcatc tccggcggcg aacagggcgc acgcaccggt cccagcatca
     1021 tgccgggtgg tgaccccgaa gcctataagg acgtacaacc cctgctggaa gcagtagccg
     1081 ccaaggtcaa cggcgtcccc tgtgtcgccc acctgggaaa aggcgccgcc ggtcactatg
     1141 taaaaatggt gcacaatggc atcgaatacg ccatcatgca gcttatcagc gagacctacg
     1201 acctgctgca ccgcggcctg ggcctcggca acgacgaact gcataagata tataagaact
     1261 ggaacgacgg cgaattacag tccttcctgg tcgagatcac ccgggatatc ttcctcaaac
     1321 aggatgacaa gaccgacaat cgcctggtag acatgatcct ggacaaagcc ggcgccaagg
     1381 ggacaggcaa atggacctcc caggatgcaa tggatctggg cgttcccatc cccgttatcg
     1441 atatggccgt ctgtctgcgc gaactctcgg cctataaaga ggaaaggacc caggccgccg
     1501 ctctttataa gaacccgtcc tcggatatcc ccgtccccag ggaagtcttt atccaacaga
     1561 cacaggacgc gctgttcttc ggcgtcatca tcagctatgc ccagggcctg gccatgctcc
     1621 atatcgcttc caaagagctg gacatgcaga tccccatcca ggaagtggtc aaggtctggc
     1681 gcggaggctg tatcatccgc tcttccctcc tcgaggtatt ttttaaagcc ttccaaaaga
     1741 accctgagct gccaaatttg ttattggaag gagagatcgc ccagttgctg caggccaaga
     1801 agcagaacat gcgcaatttg atcatccacg ccatccacat gggttatccc gtggcggggc
     1861 tgatggcggc cctgagctat ttcgacgcct atcgcagcga acgtatgccg accaacctga
     1921 tccaggcgca aagagactac ttcggagccc acacctatca gcgcatcgac atgccgggcg
     1981 tttttcatac cgaatggaat aaatagtgta tcataagaac caatagatag tttatgcaaa
     2041 accataaaag accacccgct tccctgatct ttatcttcgg gggaagcggc gacctcaatt
     2101 acaggaaact ttcccccgct ctctataact tgttcatcga cggatggatg cccgacaaat
     2161 tcggcatcgt gggcatcgga cgcagcccgt ataatgacga ggattaccgc cagcggctgc
     2221 tcaacggcat ccagcagttc agccgccgga aaggagaacc caacggccac tgggacgagt
     2281 tctcccaaca tgtcacctat ctccagatgg atgccgaaaa agaaggcgac tatcaaaaga
     2341 tcgccgatgt ggtcaagtca aaagagacag agatgggcga acaccccaat gtcatctttt
     2401 atttgtccgt agcaccgcag ctgatgccgg acatcgccca aaaactgggg gccctcaagc
     2461 tctgctccga aacatcgacc acacgcatcg tcatcgaaaa gccctttggt catgacctga
     2521 aaagcgccca tgaactgaac cagctgctca tgggcatgtt caaggaagaa cagatctacc
     2581 gcatcgacca ctacctcggc aaggagtcgg tacagaatat cctctccctc cgttttgcca
     2641 acgccctctt cgaacccatc tggagccgtc actatatcga ctatgttcag atcaccgccg
     2701 ccgaaacggt aggcgtggaa ggccgtggcg gctactacga gcaatcgggc gccctgcgcg
     2761 atatggtgca gaaccatatc ctccagctca tgtgcatgat cgcgatggag gcccccgtct
     2821 ccttcgacgc caacgagatc cggaacaaaa aggtggatgt gctgaatgcg atccgcagga
     2881 tcagcaagga agaggtcaac caggtggccg ttcgcggcca gtatggcgaa ggctggatca
     2941 aaggggaaaa ggtgccgggt tacaaacagg aaaaaagcgt caaccccaat tctgccgtcg
     3001 aaacctttgc cgccgtcaaa ttctatatcg acaactggcg ctggcaggat gtgcctttct
     3061 atgtccgcac cggcaaacgc atgaaccaga agaccacgag tatcacgata cagttcaaag
     3121 cggctcccag ctacgcattt ccgccggaag ccgcggaatc ctggcgggca aaccggctga
     3181 ccatcagcgt ccagcccgaa atggaggtcc gcctccggtt ccaggccaaa cgggccggtc
     3241 agtccatgac cctgaacccg gtggacatga tcttcaatcc aaaggacaac gaacacgagc
     3301 cggaagccta cgagaccctc ctgctcgatg tcatggaagg taaccctacc ctgttcatgc
     3361 gcgccgacca ggtggaatcc gcctggaaga tcgtgatgcc gatcctcgag acctgggaaa
     3421 cccgcgcacc cgccaacttc cccaattaca ccccggattc ctggggaccg gaagacgcgg
     3481 aagcgcttat cgccagggac ggacacgttt ggatcacgat gccgcctatc aaataatctc
     3541 agatcatgga actacacgta ttcaaagaca gcgaagcact aagcaacgcc gcggcaaaat
     3601 tcatcgccga cagcatcacc acgacgctaa agacaaagcc acgctatacc atcgcgcttt
     3661 ccggcggaag cacccccaag cggctgcacc agatcctggc ccagtccccg tataaggaac
     3721 agatcgactg gacaaagctg catgtcttct ggggtgacga acgggccgtg cccttcgagg
     3781 acagccgcaa caacgcaaag atggcatatg ataccctgct cgactttgtc cccgtccccg
     3841 catcccagat ccatgtgatg cggacggata tcggtccgga gcagtcggcc gcggaatatg
     3901 agaagatcct ccatcagtac ttcgatggcg ccgccaccag ctttgacctg gtgctcctgg
     3961 gcatgggcga tgacggacac accctctcgc tctttcccgg aacgccggtc atccacgagg
     4021 aaaagctttg ggcaacagcc ttcttcctga aagcacagga catgtaccgt atcacactca
     4081 ccaaaacgat cgtgaaccgg tcggcctgcg tggccttcct gactgccgga acggcaaagg
     4141 caaatgcatt gaaagaagtg ctgaaaggag cgtacaaccc tgatctgtat ccctcacaag
     4201 tgataaagcc ggtcggcgaa ctgcactggt ttgtcgatga ggccgcggct gctaaaatat
     4261 cttcctgatc atttcatcgc tgagtttccg atcaccctgt gtccagaaat cattgggtgg
     4321 cggaaactca ttgatattat atcccccatg ggccgcaaaa tcgaacaagg cgtgcacatg
     4381 cccgtcgatg accaatgccg ccttcatcca atcccgggcc cagatgatct ttagctgcgc
     4441 aggcgccggc ggctcctcct cttcatccgt ctcatggata tgcaaggcat ccaggaca
//
LOCUS       DATGFG010000393         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_593828, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000393 DATGFG010000000
VERSION     DATGFG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593828"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VKQ52_07435"
     CDS             <1..338
                     /locus_tag="VKQ52_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87053.1"
                     /translation="TLVSDRNILAVIVRNLVDNAVKYTISGSILVRVEKQEGGVLVQV
                     ADTGTGMSAEKVNRLLNAEQKATLESSSSFGYRFITELVRRLEGHLTIDSTPGKGTTV
                     SIYYPALAQ"
     gene            407..640
                     /locus_tag="VKQ52_07440"
     CDS             407..640
                     /locus_tag="VKQ52_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87054.1"
                     /translation="MFNKFPINFYYDGKQYSGNIQPLQTGLQNRIPTAFQVFLNNVYY
                     GLVRRKGTDWETDSPKCAIMVDIIGNHIYDRYE"
     gene            850..1086
                     /locus_tag="VKQ52_07445"
     CDS             850..1086
                     /locus_tag="VKQ52_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ87055.1"
                     /translation="MITPADKKVLKRFGENLRKLRKEKGLSLREMSYACSIDNSKIAK
                     IEKGMINITFTTLLQLAVALEAQPAVLLEYEPEI"
     gene            1207..1428
                     /locus_tag="VKQ52_07450"
     CDS             1207..1428
                     /locus_tag="VKQ52_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ87056.1"
                     /translation="MDKQQDALLKLGRRLTDLRQRQNLSLAQLSARTGLTGPEIAAIE
                     AGETDPAITTLLTLCRGLGIPPGELLPRE"
     gene            1562..1801
                     /locus_tag="VKQ52_07455"
     CDS             1562..1801
                     /locus_tag="VKQ52_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87057.1"
                     /translation="MTTLVGIFFSLISLFGLFKGKSKRNSRKLVLLGFSIFLIIGQIL
                     YMYSYLHIESQEFIGFSGLISGIILILSFQKNEAW"
     gene            1837..>2019
                     /locus_tag="VKQ52_07460"
     CDS             1837..>2019
                     /locus_tag="VKQ52_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87058.1"
                     /translation="MRTVILSAACCLISLFASTQPRSIYHDGWIDFNKNGTKDIFEDP
                     AQPVERRIADLLSQMTL"
BASE COUNT          520 a          600 c          436 g          463 t
ORIGIN      
        1 tgaccctggt atccgaccgg aatatcctcg ccgtgatcgt ccgcaacctg gtggacaatg
       61 ccgtaaaata taccatctcc ggcagtatcc tcgtccgggt ggaaaaacag gagggcggtg
      121 tcctggtcca ggtggccgac acagggaccg gtatgtccgc ggaaaaggtc aaccgcctcc
      181 tgaacgccga acaaaaagct acgctcgaat ccagttcttc cttcggctac cggttcatca
      241 ccgaactggt ccggcggctg gagggccatc ttactatcga cagcaccccg ggaaaaggga
      301 ccaccgtgtc catctattat cccgccctcg cacaataaat aaaagacgga gaggttttac
      361 agttggcacg atttattctc tataccttct gaaaaaaacc tattttatgt tcaataaatt
      421 cccgatcaac ttttattacg atggcaaaca atacagcggt aatatccagc ccttgcaaac
      481 aggtcttcaa aaccgcatcc ctactgcctt ccaggtattc cttaacaatg tctactacgg
      541 cctggtcaga agaaaaggta cggactggga aacggattcc ccaaaatgtg ccatcatggt
      601 ggacatcatc ggcaatcata tttatgatcg ctatgaatga ttcctcccaa cccggccccc
      661 ggccgggctt tcctcttttg gttcctctat tacggtaccg ctgattcgaa gatcgccgtc
      721 gcaatccgcc cggagaaatg acgggttcgt ggatgaacga attattaaat cacttttgtg
      781 cggccttatt ggaccacaat gatcttgaaa aaactttaca ccttaggact ccattagaag
      841 gaaaacataa tgataacgcc agccgataaa aaggtactga aaaggttcgg agagaacctc
      901 agaaaactcc gtaaggaaaa aggacttagc ctccgtgaga tgtcgtatgc ctgtagcatc
      961 gacaatagta agatcgccaa gatcgaaaag gggatgatca acatcacctt caccaccctg
     1021 ctgcaactgg ccgttgccct cgaagcccag cccgccgtcc tgctggaata cgagccggaa
     1081 atctgattct ccctttccca atggttacca acgggctgag cccgtaatag gtaggaccac
     1141 tccgcatgga gttgttcctc tattcatctt gctgtccaac cccatactct tatcacaacc
     1201 accgctatgg acaaacagca agatgccctg ctcaagctcg gccgccgcct gactgacctt
     1261 cgccagcgac aaaacctctc cctggcgcaa ctttccgcgc gcaccggcct caccggcccg
     1321 gagatcgccg ccatcgaagc cggggaaaca gaccccgcga tcaccaccct cctcaccctc
     1381 tgccgcggcc ttggcattcc ccccggcgag ctcctgcccc gggagtgacc ccgatccgta
     1441 ttcttacggc ggaaatccac tattgcaggg tgggcggggg gagcttatct taccaaagat
     1501 gaccgatagt catccggcca aaggccaatc gtatatcctt aactatgaaa aaccaataac
     1561 tatgacaacg ctggtgggta tcttcttctc ccttatctcc cttttcggcc tgttcaaagg
     1621 caaatccaaa cgcaacagcc gtaaactcgt ccttctcggc ttttccatct tcctgatcat
     1681 cggccagata ctctatatgt acagctacct tcacatcgaa agccaggaat tcatcggctt
     1741 ctcgggcctg atcagcggaa taatcttaat cctttccttt caaaagaacg aagcatggta
     1801 atttcttaaa acgcattttc agcagtattt ttacggatgc gcaccgtaat cctgtcagcc
     1861 gcttgctgcc ttatcagtct ttttgcttcg acccagccaa ggtcaattta ccacgacgga
     1921 tggatagatt tcaacaagaa cggtactaaa gacatcttcg aagacccggc gcaacccgtc
     1981 gaacgtcgca tcgccgacct cctcagccag atgaccctc
//
LOCUS       DATGFG010000394         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1165361, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000394 DATGFG010000000
VERSION     DATGFG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165361"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2435
                     /locus_tag="VKQ52_07465"
     CDS             <1..2435
                     /locus_tag="VKQ52_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ87059.1"
                     /translation="TLDMLPAREEVLKTSAGDDPLSVYKLKMSYGYHYQYSRNCLQLN
                     VGEGMRFSEIGLYAGIAATDWSWSPLAADFDNDGQKDIFISNGIQRRPNDLDVIKFMS
                     SGTVASALRKGRAADPATMQQMPPGKVSCFMFQGTDSLVFTDRSKEWGFDHPAFSNGA
                     AYGDLDNDGNLDLVVNTVNEPAMIYHNTGAGKDRHYLDVELRGPAGNVFGIGAKVAIR
                     SWGKLQYGYCSVTRGFESGSLGPVHFGLGGQTRVDTLEVIWPDGKMQRMTDVGVDRRL
                     VVHYRDAVVGHGGMLPVSVKGGGLLAEVTDSIGLPYRRVEDRFNDLDVQPLMPHQQST
                     RGPRLAVADIDGDGLDDLFICGGVRQPGSLFRQEASGRFVPVDTALFKADSFCPDADA
                     VFADLNGDGHPDLVVVSGGNEYPDGSAGLVDRVYFNTGDGHFRKGPVLPGIPENKAVV
                     VAADVDHDGDVDLFVGGSSFYGKIGEAPVSYLLLNNGKGNFTVADEAKAPGLSRIGQV
                     TSAVWADLDKDGWEDLVVVGEWMPVTVFMNHAGRLENRTEALGLGHTTGWWTSVCAAD
                     VNGDGKPDLLAGNWGENSKLTASSRYPLKLYVGDLDNNGVEDPVLAVARDGQYYPFLG
                     KDELEKHFPALIRKRYNSYAEFAGKTVEEVFGDGLDKMTVLKAECLSSVMLTNTGANG
                     FTVSRLPNEVQWSPVFTFVTGDWNRDGVMDIIAAGNFYGVLPYEGRYDASYGSLLLGE
                     GIMGSGRSGFCGDASGDCRPGFHVLPMSRSGILLEGEVRDGKVLRTARGPLLAFSRNA
                     GGVVFYRGRQIP"
BASE COUNT          470 a          500 c          964 g          636 t
ORIGIN      
        1 ttacgctgga tatgttgccg gcgcgggagg aggtgttgaa gacgtcggcg ggtgatgatc
       61 cgctcagcgt gtataagttg aagatgagct atgggtatca ttatcagtat tcgcgtaatt
      121 gtcttcagct gaatgtcggc gaggggatgc gattcagtga gatcgggctt tatgccggga
      181 ttgcggcgac ggattggagc tggagtccgc tggcggcgga ttttgacaat gacgggcaga
      241 aggatatttt tatctccaat ggtatccagc ggcgccccaa tgacctggat gtcatcaaat
      301 tcatgtcttc gggaacggtg gcttccgctt tgcggaaggg gcgggcggcc gatccggcga
      361 cgatgcagca gatgccgccg ggtaaggtaa gctgttttat gttccagggg acggatagcc
      421 tggtgtttac ggataggagt aaggaatggg gttttgacca tccggctttt tcgaatgggg
      481 cggcttatgg cgatctggat aatgacggga atctcgatct tgttgtcaat acggtgaatg
      541 agcctgcgat gatctatcat aataccgggg ccggtaagga ccggcattat ctcgatgtcg
      601 agttacgggg gccggcgggc aatgtttttg gtattggcgc caaggtggct atccgctcct
      661 gggggaaatt gcaatatggg tattgttcgg tgacgcgggg gttcgagagc gggtcgcttg
      721 ggccggtgca ttttgggctt ggagggcaga cgcgggtaga tacgctcgaa gtgatctggc
      781 cggatgggaa gatgcagcgg atgacggatg tgggggtgga tcgccggctg gtggtgcatt
      841 accgtgatgc ggtggtgggg catgggggga tgttgccggt tagtgtgaag ggaggtgggt
      901 tgcttgcgga ggtgaccgat tcgatcgggt tgccctaccg gcgggtggag gaccggttca
      961 acgatttgga tgttcagccg ttgatgccgc atcagcaatc gacgcggggc ccgagactgg
     1021 ccgttgcgga tatcgatggc gatgggcttg acgatctttt tatttgtggc ggggtgcggc
     1081 agccggggag tcttttccgg caggaggcat cggggcgttt tgtgccggtg gacacggcgt
     1141 tgttcaaggc ggacagcttt tgtccggatg cagatgcggt gtttgcggat ctgaatgggg
     1201 atgggcatcc cgaccttgtt gtcgtaagtg gggggaacga gtacccggat ggatcggcgg
     1261 ggctggtcga ccgggtctat ttcaataccg gggatgggca tttccgaaag gggccggtgt
     1321 tacccggcat ccccgagaac aaggcggtgg tggtggcggc ggatgtggat catgatggag
     1381 atgtggatct gtttgtcggc ggcagcagtt tttatgggaa gatcggggag gcgccggtgt
     1441 cgtacctttt gcttaataat ggcaagggga attttactgt tgccgatgag gcgaaggcgc
     1501 cggggctgtc gaggatcggg caggtaacga gtgcggtgtg ggcggacctg gacaaggatg
     1561 gctgggagga tcttgtcgta gtgggcgagt ggatgccggt gacggtgttt atgaaccatg
     1621 caggccggtt ggagaaccgg acggaggcgc ttgggcttgg gcatacgacc ggctggtgga
     1681 cgagtgtttg tgcggctgat gtcaacgggg atgggaagcc cgatctgctg gcgggtaact
     1741 ggggggagaa ttcgaagctg actgcttctt cgcgttatcc tttgaaattg tatgtgggcg
     1801 atctggacaa taatggggtg gaagatcctg tactggcggt ggcgagggat gggcaatatt
     1861 atccatttct tggcaaggac gaattggaga agcatttccc tgcgttgatc cggaagcgtt
     1921 ataacagtta tgcggaattc gcgggtaaga ccgttgaaga ggtgtttggg gacggcctcg
     1981 ataaaatgac agtgctgaag gcggaatgtc tctcgtctgt gatgctcacc aataccgggg
     2041 cgaatggatt tacggtcagc cggcttccga atgaagtgca gtggtcgccc gtctttacct
     2101 tcgtcacagg tgactggaac agggatgggg tgatggatat cattgccgcc gggaattttt
     2161 atggggtgtt accctatgag ggccggtatg acgccagtta tggatcgctc ttgcttggcg
     2221 aggggatcat gggtagcgga agatcagggt tttgcgggga tgcgtcgggc gattgccggc
     2281 cgggttttca tgtcttgccg atgagccggt cggggatatt gctggaaggg gaggtgcggg
     2341 atgggaaggt attgcgaact gcgcgcggtc ctttgctggc tttctcccga aatgccggcg
     2401 gggtcgtctt ttacaggggg agacagatcc cgtagtggtg gtatcttcaa gaggggaatt
     2461 tggtgtaata ttgtggccat gcgaaccctt tgtttatcct tgtgtttact ggcggcgacg
     2521 gctgtttttg cccagggcgg caatgagcct gtcgaccggg atattgtgcg
//
LOCUS       DATGFG010000395         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_366419, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000395 DATGFG010000000
VERSION     DATGFG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366419"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VKQ52_07470"
     CDS             <1..1084
                     /locus_tag="VKQ52_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ87060.1"
                     /translation="LGDGRGHFVRSADALPEMRVAKSCVRAADINGDGYPDLFVGGRV
                     IPGLYPVTPPSYLLINDGKGHFADRTAELAPALQKLGMVTDAAWADLRGDGKKELIVV
                     GEWMPVTVFQVEKGRLADHSRDYFDQEYSGWWNRLLVDDLDKDGRPEIVIGNLGSNTQ
                     CRADERHPAELYYKDFDGNGTVDPVLCYYIGDTSYPFMTRDELISQVSNMSQRFPDYK
                     SYADARLTDIFPPEALKGAGHLRATCLRTLYLKSGRDGRFHEQPLPVQAQYSPVFTIT
                     PLDYDGDGNEDLLLCGNTTHARLRFGKYDANYGVLLKGDGKGQFRYIDQPVSGFHLRG
                     DVRSVADWRGELLFGVNGVGVRAYRR"
     gene            1175..2350
                     /gene="galK"
                     /locus_tag="VKQ52_07475"
     CDS             1175..2350
                     /gene="galK"
                     /locus_tag="VKQ52_07475"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HLZ87061.1"
                     /translation="MIATDFVPALSSRVAQKFQEVFASNGALLVRSPGRVNLIGEHTD
                     YNNGFVLPAAIDKAIYLAIERRRDAEVHLFAMDLDQSYRGPIQPLAPSALHWPNYILG
                     VVEQLQRMGHVIGGFNCVFAGNIPLGAGMSSSAALECATAFALNQTFGLGLDPMTMVK
                     LSQKAENEFVGLQCGIMDMFASMFGRKDQVIRLDCQSLDYTYFPFKTDGLRILLLDTQ
                     VEHSLASSEYNVRRQQCEAGVKLVQRHHPGVKSLRDVTTDMLAAFVAPADELIYRRCS
                     FVVEENERVLAGTDDLQRGDVAAFGQKMYASHEGLSRKYEVSCPESDWLVEQVRGAEG
                     VLGARQMGGGFGGCTINLVKAGVIEGLVAGIAPLYRKAMNRELKWYIGQIENGTSPV"
BASE COUNT          468 a          594 c          800 g          529 t
ORIGIN      
        1 cctgggcgac ggcagggggc attttgtccg gagtgccgat gcgctgccgg agatgcgggt
       61 agccaagagc tgtgtacgcg cggcggatat caatggcgat ggatatcccg atctttttgt
      121 cgggggcagg gtcatcccgg ggctttaccc ggtgacgccg ccgagctatc tgctgatcaa
      181 cgatggaaag gggcattttg cggaccgtac ggcggagctt gcgccggccc tgcaaaagct
      241 gggaatggtg accgatgcgg cctgggccga tctcaggggg gatgggaaga aggagctgat
      301 cgtggtcggg gagtggatgc cggtgacggt attccaggtg gagaagggcc gtttggcgga
      361 tcatagccgg gactattttg accaggagta cagcggctgg tggaacaggc tgctggtgga
      421 cgacctggat aaggatggcc ggccggagat cgttatcgga aatttaggat cgaacaccca
      481 atgccgggcg gatgagcggc atccggccga actgtactat aaagacttcg acggcaatgg
      541 tacagtggac ccggtgcttt gctattatat cggggatacg agctatcctt ttatgacccg
      601 ggatgagctg atctcgcagg tgagcaatat gagtcagcga ttccccgact acaagagtta
      661 tgcggatgca cgcctgaccg atatcttccc gccggaggcc ttgaagggcg ccggtcatct
      721 gcgggctacc tgtctgcgga cgctgtacct gaaaagcggg agggatgggc ggtttcatga
      781 gcagccgctg ccggtgcagg cgcaatattc acccgttttt acgatcaccc ccctggatta
      841 tgacggggat gggaatgaag acctgttgct ttgcgggaat actacgcacg cgcggttgcg
      901 gtttgggaaa tacgatgcca attatggggt gttgttgaaa ggggatggga agggacagtt
      961 ccgctatatc gatcagccgg tatcggggtt ccatctgcgg ggggatgtgc ggtcggtagc
     1021 ggattggcgg ggggagctgc tttttggggt caatggagtg ggtgtcaggg cgtaccgaag
     1081 atgaacgggc ggactgttgc ggggccaaag ttttttttat tttccgtcaa aatagatgtt
     1141 attttgacat ttgcagcacc gccatatttc acttatgatc gcaacagact ttgttcccgc
     1201 gctttcctcc cgggtagccc aaaaattcca ggaagtattt gccagcaatg gcgccttact
     1261 cgtccggtcg cccggccggg tcaatctcat cggggagcat accgactaca acaacgggtt
     1321 tgtcctgccc gccgctatcg acaaggctat ttacctggcc atcgaacggc ggcgtgacgc
     1381 ggaggtacat cttttcgcca tggacctgga ccagtcgtat cgcggaccga tacagcccct
     1441 ggcgccgtcg gcgctgcact ggccgaacta tatcctcggg gtggtagagc agttgcagcg
     1501 gatgggacat gtcatcggtg gttttaactg tgtttttgcc ggcaatatcc cgcttggggc
     1561 ggggatgtcc tcctcggcgg cgttggaatg cgccacggcc tttgccctga accagacctt
     1621 cgggcttggg ctcgacccga tgacgatggt aaagctttcg caaaaggccg agaacgaatt
     1681 cgtgggattg cagtgcggca tcatggacat gtttgccagt atgttcggcc ggaaggacca
     1741 ggtgatccgg ctggactgtc agtcgctcga ctatacctat ttccctttca agaccgatgg
     1801 cttgcgcatc ctgttgctcg atacgcaggt ggagcattcc ctcgcctcct ccgaatacaa
     1861 tgtccggcgc cagcagtgcg aggccggggt gaagctggtg cagcgtcacc atcccggggt
     1921 gaagagcctg cgggatgtta ctacggatat gctggccgcc tttgtcgcgc cggcggacga
     1981 gctgatctac cgccgttgca gttttgtggt ggaagagaac gagcgggtgc tcgcggggac
     2041 ggatgatctg caacgcggtg atgtagccgc ctttggccag aagatgtatg cctcgcacga
     2101 agggcttagc cggaaatatg aggtcagctg tcccgagtcg gactggctgg tggaacaggt
     2161 cagaggggcg gaaggcgtcc tcggcgcccg ccagatgggt ggcggctttg gcggttgtac
     2221 gatcaacctg gtgaaggccg gtgtcatcga agggctggtg gcggggattg cgccgctgta
     2281 ccggaaggcc atgaaccggg agctgaaatg gtatatcggc cagatagaga acggcacatc
     2341 ccccgtgtaa taaattccat tgctatatga acaaattctt tcttgctccc c
//
LOCUS       DATGFG010000396         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_868841, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000396 DATGFG010000000
VERSION     DATGFG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868841"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="VKQ52_07480"
     CDS             complement(<1..1914)
                     /locus_tag="VKQ52_07480"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HLZ87062.1"
                     /translation="MEDHNTRYFGRDKLARTCIDRLLERFDDGSRVAVLRLTGSSGSG
                     KSSLLRAGILGGLTAPEHRGRYYVAAVRPEDLHDAGGREGSVIPSILNAIVKKAGLDI
                     SGADILDITDKGSGAPKRAAEIISRELRRRPNRDARLVLGLDQFEEIVDILAGREPGD
                     WHILLQFVEEAIRHPNIGFVYTLESSRKHQHDQLELGNAFKFAHEEELDLDRDFIDEI
                     IRKPFADTGYALANDVVDTLKRNLHELQDEKDHLAYHSVLPLLAVRLYFLWEFVSKRF
                     EPGTSEGNIPFDLSPNLHNTITVAQLQETDRTVLEFKDIIQQQAELAWKKAGIGSIQD
                     EQLEFFLQPLVGVENGRLKLIVGSRLAPYRNERALLRSFMRHGLIVGAGDGTVRFVHE
                     AVLMHWKEAKEWFDQRQEFLEMKARLRSRALAWDSDRRPKIRRTRDTEKEIAEAVEVL
                     WRYIRHWSIEQVDPLYQQDPLYEYCLTIFSLSRTPRALSLSFPKIAYNHATLAATYGL
                     VPLLKRYARLDAQSLLLVNEYGQKKTPLIQAAWGQLEAVQFLLDHGADPFLPDKNGWL
                     PCHAPIQMHRQDIFTALMDAAAGKSAKGHLEKRLLCPDNQTFLHIAAEYDNYDAAHDL
                     IHRYGFSPSSKSAL"
     gene            complement(1803..2741)
                     /locus_tag="VKQ52_07485"
     CDS             complement(1803..2741)
                     /locus_tag="VKQ52_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87063.1"
                     /translation="MMVSEQLNGEIGADGFLKPGHPFLLIAGSSDVREEMGIAQQTVQ
                     EVLREYGIHDVAAYVWDVQAGPEGLDQRRTMQRNIPRPSSDECKGVIYIVGERFGLPM
                     EEGFDISVIGNIEDWTQNGHYCLSPTWPNEAAEERQLLEKGYFPLTGGVFEFLDGRGY
                     RSDDHPEGKPIWLCLLAERPIERENEWMALNGSRWFNERTKDMTPAERTRFQEGEYKR
                     HWLAVHNLMHALAERGISQNPMPTPDKLRRGYAASSHRRSAGPAAFTAIPTASFPTMI
                     SRITTPGTSAGINSPGPASTGCWNVLMMGPGSPSSG"
     gene            2790..3026
                     /locus_tag="VKQ52_07490"
     CDS             2790..3026
                     /locus_tag="VKQ52_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87064.1"
                     /translation="MKKITNITHILLAAIATGGIGLPQPLQAQSQPPRPTQPASLAQS
                     ARILTAVLAYQSEPRPVNFCSIGKFFPADYFAGL"
     gene            3023..>3561
                     /locus_tag="VKQ52_07495"
     CDS             3023..>3561
                     /locus_tag="VKQ52_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87065.1"
                     /translation="MNAKDLRPLFVCWLLVVGMRVAAQPPAADHPGKIQNNWIVFIFE
                     QDGKGLTTAYPRYFLLYTDGTVHQFAPGEQPQPFEGIDQVISLASSDKHVVALRRDGT
                     VWTWGNNESGELGYPTPGKVPWAYHSSPMQVKGIAHAVAVSAKYNCSNALLADGTVWT
                     WGAKAPGHYLPDRSRWNYT"
BASE COUNT          759 a         1026 c          954 g          822 t
ORIGIN      
        1 tagcgcactc ttactggaag gggagaaccc atagcggtga atgaggtcat gtgccgcatc
       61 gtaattgtcg tattccgcgg cgatatgcag gaaggtttgg ttatccggac acaagagcct
      121 cttctccaaa tggccctttg cgctctttcc ggcggcggca tccatcaagg ccgtaaagat
      181 atcctgccgg tgcatctgga taggggcatg acaaggtaac caaccatttt tatccgggag
      241 aaagggatcg gcgccatggt ccaggagaaa ttgcacagcc tccaactgac cccaggcagc
      301 ctgtatgagg ggcgtcttct tctgtccata ttcgttaaca aggagaagac tctgtgcgtc
      361 cagtcgggcg tagcgcttta aaaggggtac caggccatag gttgctgcca acgtagcatg
      421 attgtatgcg atcttaggga aggagagact caaggctcgt ggggttcgac taagcgaaaa
      481 gatggtgagg caatattcgt atagggggtc ctgttgatac aatgggtcta cttgttcgat
      541 cgaccaatgc cggatatacc tccagagcac ttcaaccgcc tccgcgatct ctttttcagt
      601 atccctggtg cgtcttattt ttggccttcg atccgaatcc caggccaatg cccttgagcg
      661 aagcctggcc ttcatctcca ggaactcctg acgctgatcg aaccattcct ttgcttcctt
      721 ccagtgcatc aggactgctt cgtggacgaa gcggaccgtt ccgtcgccgg cgccgacgat
      781 gagtccgtga cgcatgaaac tgcggaggag tgctcgctca tttctgtaag gggccaaccg
      841 ggaaccaaca atcagtttca gcctaccgtt ctcgacaccg acaagcggtt ggagaaaaaa
      901 ctcgagctgc tcatcctgga tcgaccctat accggccttt ttccacgcga gctctgcctg
      961 ttgctggatg atatccttga attccagtac ggttctgtct gtttcttgca actgggcgac
     1021 cgtgatcgtg ttatggagat tgggtgagag atcgaatggt atattaccct cgcttgttcc
     1081 tggttcgaac cgcttactga cgaattccca taaaaaatag aggcgtacag caagaagcgg
     1141 caacaccgaa tggtaggcaa ggtggtcttt ctcgtcctgc aattcatgca ggttccgctt
     1201 gagcgtatcg acgacatcat tcgccagggc atagcccgtg tctgcaaaag gtttgcggat
     1261 gatctcgtcg atgaaatccc tgtcgaggtc cagttcttct tcatgggcga acttaaaggc
     1321 atttcccagc tctaactggt catgctgatg ctttcggctg ctttccagcg tgtaaacaaa
     1381 accaatattg ggatgacgga tcgcctcttc cacgaattgg aggaggatat gccagtcgcc
     1441 gggttcccga ccggccagta tgtctacgat ctcctcgaat tgatcgagcc ccaggacaag
     1501 ccgggcatca cggttcggcc gtcgccgcaa ctcccgggag atgatctcgg cggccctttt
     1561 cggagcgccg ctccctttgt cggtgatatc aaggatatcg gcgccactga tgtccaaccc
     1621 ggcctttttt acgatcgcat taaggatcga agggatcacc gatccctcgc gcccgccggc
     1681 gtcatgaaga tcttccggcc tgacggccgc cacatagtag cgtccacggt gctccggggc
     1741 ggtaagacct cccaggatac ctgccctcaa caaagatgat ttcccactgc cactggagcc
     1801 cgtcagccgg aggacggcga cccgggaccc atcatcaaaa cgttccagca gccggtcgat
     1861 gcaggtcctg gcgagtttat cccggccgaa gtaccgggtg ttgtgatcct cgatatcata
     1921 gtaggaaagc ttgcggtagg gattgctgtg aaggccgcgg ggccggcaga tcttcgatgc
     1981 gacgaagctg cgtatccccg ccggagttta tccggcgtcg gcatcgggtt ctgactgatc
     2041 cccctttcgg cgagcgcatg catcaggttg tgtacggcca gccaatggcg cttatattcc
     2101 ccttcctgga agcgggtccg ttccgccggt gtcatgtctt tcgtacgctc gttgaaccac
     2161 cggctcccgt tcaacgccat ccactcgttc tcccgctcga tcggcctttc ggccaggagg
     2221 cacaaccaga tgggcttccc ttcgggatgg tcgtcacttc gataaccccg gccgtccagg
     2281 aactcaaaaa cacctcccgt cagcgggaaa tagccttttt cgagaagttg ccgctcctcg
     2341 gcagcctcat tgggccatgt aggggacagg caatagtgac cgttttgggt ccagtcctca
     2401 atattgccaa taacgcttat gtcaaaaccc tcttccatcg gcaacccgaa ccgctcaccg
     2461 actatataga tcacgccctt acattcgtca gaagatggcc gggggatatt gcgttgcatg
     2521 gtacggcgtt gatccagccc ttccggtccc gcctgcacat cccaaacata ggcagccaca
     2581 tcatgaatgc catactcgcg gaggacctcc tgtacggttt gttgcgcgat gcccatttct
     2641 tccctgacgt cgctcgaacc cgcaatgagt aggaagggat gtccaggttt caggaacccg
     2701 tctgcgccga tctccccgtt aagctgttcg ctcaccatca tgataattga ttgcttgatt
     2761 cgtatattta tataagttaa acgccatcta tgaaaaagat aaccaatatt acccatattt
     2821 tattagcggc gatcgccacc ggcggcatcg gccttcccca gcccctgcag gcccaatccc
     2881 agcccccccg gcccacccaa cccgcctcgc tagcgcagtc tgcccgcatc ctcaccgcgg
     2941 tactcgccta ccaaagcgag ccacggccgg taaacttttg ttctataggc aaattttttc
     3001 cggcagatta ttttgccggc ctatgaacgc aaaagatctc aggccgctat ttgtttgctg
     3061 gttactggta gtgggaatgc gggtggccgc gcaaccgccc gcggcggatc atcccgggaa
     3121 aattcaaaac aactggatcg tgttcatctt cgagcaggat gggaaagggc tgaccaccgc
     3181 ctatccgcgg tatttcctgt tatatacgga tgggacggta catcaatttg cgccgggcga
     3241 gcagccccag cccttcgagg gtatcgatca ggtgatctcc ctggcatcca gcgataaaca
     3301 cgtcgttgcc cttaggcggg acggaacggt atggacctgg ggcaataacg aatcgggaga
     3361 attgggatat cccacgccgg ggaaagtccc ctgggcttac cactcctcgc cgatgcaggt
     3421 gaaggggatt gcccatgccg tcgccgtttc cgcgaaatac aattgcagca atgcgcttct
     3481 cgcggatggc acggtgtgga cctggggggc aaaagccccc gggcattatt tgcccgatcg
     3541 tagccggtgg aactacacag a
//
LOCUS       DATGFG010000397         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_822215, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000397 DATGFG010000000
VERSION     DATGFG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822215"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="VKQ52_07500"
     CDS             <1..1627
                     /locus_tag="VKQ52_07500"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HLZ87066.1"
                     /translation="KRRDCNNEAMEKTFRFTTGSGRLLDVIFRAYDNGVAFRYAFPQT
                     PADTRRVTGERTSFVIPEGTERWTQQYTPAYEALYPSATTGKADAPDHQQWGFPVLYK
                     IQDKPVWVMISEAGVSRDNCASRLSNKDADTIYRVTMPEDVIPAGQGWKSAWRVVMIG
                     SLADIVQSTLITDVSAPSKLADTSWIRPGAVAWEYWAYNHGSKDYQKIVEYVNLAKAM
                     HWPYVLIDWEWDRMGNGGNIENAVAYARSKGIKPLIWYNSRDSLSSGAGLDPYGRLST
                     HEARCKEFAWLNKIGVYGVKVDFFEDDRQQEMAYYLDLLADAANYHLMVDFHGATIPK
                     GWDRTYPNLMTMEAVYGAEWYAYAPVLTQQGARHNATLPFTRNVVGPMDYTPVTFTNS
                     NFSHTTTYAHELALSVVFESGLQHFADRPEGFYALPDAERRFLMTVPVAWDDTRLIDG
                     FPGKRVVIARRKGAAWYIGGLNGEDQPQTLKLSFDFLPPGAYHLELIRDGATGKEFTT
                     QTVAVTKGSIVKVNCLPRGGFGATLVPAQHPGR"
     gene            1809..>4265
                     /locus_tag="VKQ52_07505"
     CDS             1809..>4265
                     /locus_tag="VKQ52_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ87067.1"
                     /translation="MKKRNLKRALLRLLWRTLSLLLFSAGGYAQNPLTPKKITGKVVV
                     APGDKPLEGASVTVRGRAGGTQTDKNGEFSLDAKDGDVLIISYVGYLHSEVRVGARQV
                     INVRLQVEDRGKLNDVVVIGYGKARRGDVTGSISSISGTELMKTQPTTFDQALQGKVA
                     GVVVQQVSGQPGGGVSIQIRGVSSISGTNSPLYVIDGVIIPPVNDPGNGSNPLNTINP
                     AEIESIDVLKDASATAIYGSQATNGVVVITTKRGRAGAPLINYDAYAGYQELPKRLPT
                     MNLQQFATFINARAQVWGFDSRPEFANPKYLGPGTDWQKELFRKAPDMSHTVTVSGGD
                     SRTQYLLSGSYFDQKGMALGSDFQRISVRLNLDNKTTSWLKVGTSLQMAHVFENVNTT
                     ASGVISSALNLTPDIPVQNTDGTWGGITNTSGWVTPVVNPVAIAQLVKNQRKRNQLFG
                     NAYAEVQFYKDLTLRNEVSGNFDFATQDNFTPTYTFGKAVNGTNSGSTSISQNIYSVI
                     RNYLTYNHYFTKFNVNALVGHEAQTSSFENSGGSRSNFPTNNIQALNAGDATTARNSG
                     DNGLGSSLESYFGRINFSWDDKYLVTGNVRNDGSSNFPVNNRWVTTWSGAFAWKINNE
                     SFLKGVRDINELKLRLGYGLTNNQGIPGNTYVTQLQTVANGLSGIAQYQSNLANPNVT
                     WEKTNYYNGGLDATVLNGRVSFSLDAYYRKTDGLLLKVPLPLYSGTTAGYSPGSMAAP
                     YVNVGAVSNKGFDFRVSTTNVNGRNFTWKTDVTVSRNVNKVLNLGAGDQANLSQKSYV
                     INDIVEKTVVGQPIGEFYGYI"
BASE COUNT         1078 a         1150 c         1139 g          898 t
ORIGIN      
        1 caaacgccgc gactgcaata acgaggcgat ggagaagaca ttccggttca cgaccggcag
       61 cggccggctc ctcgatgtca tcttccgcgc ctacgacaac ggtgtagcct tccgctacgc
      121 ttttccccag accccagccg acacccgccg cgtgaccggc gagcggacca gcttcgtgat
      181 ccccgaaggc accgaaagat ggacacagca atataccccc gcttacgaag ccctctatcc
      241 ttcggccact acggggaagg cggatgcgcc cgatcaccag cagtggggat ttccggtatt
      301 atacaaaata caggataagc cggtgtgggt gatgatctcg gaggccggtg tcagcaggga
      361 caactgtgct tctcggcttt ccaataaaga cgccgacacc atctataggg ttacgatgcc
      421 cgaggacgtc attcctgccg gccagggctg gaagtccgcc tggcgggtgg ttatgatcgg
      481 cagccttgcg gacatcgtac agtcgacatt gatcaccgat gtaagtgcac cgtcgaagct
      541 ggcggatacc agttggatcc ggccgggggc cgtggcctgg gagtattggg catacaacca
      601 tggttcgaag gactaccaaa agatagtcga atacgttaac ctcgcgaaag cgatgcattg
      661 gccttatgtg ctcatcgact gggaatggga ccggatgggc aacggcggta acatcgaaaa
      721 tgccgtcgcc tatgcgcgga gcaaaggtat aaaaccgttg atttggtaca attcgaggga
      781 ttccctgagc tcgggagcgg ggctcgaccc gtatggaaga ttatcgacac acgaagccag
      841 gtgcaaggaa tttgcctggc tgaacaagat cggggtatat ggcgttaagg tcgacttctt
      901 cgaggacgac cggcagcagg agatggccta ctatctcgac ctgctggcgg atgcggcgaa
      961 ctaccatctc atggtggatt tccatggggc cacgataccc aaaggatggg atcgaactta
     1021 ccccaacctc atgaccatgg aggccgtata tggcgcggaa tggtatgcct atgcacccgt
     1081 cctgacgcaa caaggcgcgc gacacaacgc gaccttaccg tttacccgga atgtggtcgg
     1141 accaatggat tatacgcccg tgaccttcac caattccaac ttctcgcata cgaccaccta
     1201 tgcacatgag ctggcgttgt cggtggtgtt cgagtcaggg ctgcagcatt ttgccgaccg
     1261 gccggaaggt ttttatgccc tgcccgatgc ggagcgtcgg ttcctgatga cggtgccggt
     1321 cgcatgggac gacacccggc ttatcgacgg ctttcccggg aagcgcgtag tgatcgcccg
     1381 ccgaaagggg gccgcatggt atatcggtgg gttgaatggc gaggaccagc cgcaaacctt
     1441 gaagctctcc ttcgatttcc taccccctgg cgcgtaccat ctcgaactta tccgcgacgg
     1501 cgccaccggc aaggaattca ccacccaaac agtcgcggtc acgaaaggaa gcatagtgaa
     1561 agtcaactgt ttgccccgcg gcggattcgg cgcaacctta gtgccggcac aacatcctgg
     1621 ccgttaacca atgataccct aaaaataccg gaatgatacc cctccgcaaa acaatctgac
     1681 cccctcattt gctagctgcg ctaccccgcc tatcgacata taaaattatt tttgaatcga
     1741 tgcggtaacc acttgctgcc ccgtacgagg gttgccgatt gtaaagacaa aaccaagacg
     1801 attgcaatat gaaaaagcga aatctcaaaa gagcgttgct gcgcttgtta tggcgcacgc
     1861 tcagtcttct tctcttttct gccggcggat atgcacaaaa tccgctcact cccaaaaaga
     1921 tcaccggaaa agtagtggtc gccccaggcg acaaacccct cgaaggcgcc agtgtcacgg
     1981 tcaggggacg cgccgggggg acacaaaccg ataagaacgg cgagttcagc ctggatgcca
     2041 aggatggtga tgtgctgatc atcagctatg taggctattt acatagcgaa gtcagggtgg
     2101 gagcccggca ggtgatcaac gtccgcctgc aagtggaaga tcggggcaag ttgaatgatg
     2161 tcgttgtcat cggttatgga aaagcgcgcc gcggggacgt gaccggatct atttcttcca
     2221 tttcaggtac ggagctaatg aaaacccagc cgacgacatt cgaccaggcc ctgcagggaa
     2281 aggtcgcggg cgttgtggtg cagcaggtgt cgggacaacc cggtggtggc gtatcgattc
     2341 aaatacgcgg cgtgtcttcg ataagcggca cgaattcgcc attatatgta atagatggcg
     2401 ttattattcc tccggtcaac gaccccggca acggttctaa tccgttgaac acgatcaacc
     2461 ctgcggaaat agaatcgata gatgtgctga aagacgcctc tgcaaccgct atatatggtt
     2521 cgcaggcaac caacggcgtc gtcgttatta ctacaaaaag aggcagggcg ggggcgccgc
     2581 tgatcaatta tgacgcctat gccggatacc aggagctacc gaaaaggctg cctaccatga
     2641 acctgcagca gtttgccacg tttatcaacg cgcgggcaca ggtgtggggc ttcgactcca
     2701 gacccgaatt cgccaatcca aaatatttag gccccggaac cgactggcaa aaagaattat
     2761 tccgcaaagc gccggatatg agccataccg ttactgtcag tggtggcgat agccggacac
     2821 aatatttatt atcgggctct tatttcgacc aaaaaggtat ggcgctaggc tcggattttc
     2881 aaaggatttc ggtacggctg aacctggata ataagaccac cagctggcta aaagtaggaa
     2941 ccagtctcca gatggcgcac gttttcgaaa atgtgaatac caccgcctca ggcgtgatca
     3001 gttcggcact gaaccttaca cccgatattc cagtacaaaa caccgacggc acctggggcg
     3061 gtatcaccaa caccagcggt tgggtgacgc cggtcgtcaa cccggtggcg atagcccaac
     3121 tcgtaaagaa ccagagaaag agaaatcagc tgtttggcaa tgcatatgcc gaggtacaat
     3181 tctataaaga tttgaccctg cgaaatgaag tatccggtaa tttcgatttt gccacgcagg
     3241 ataattttac gcccacctat accttcggca aggccgtcaa tggtacaaac agcggatcca
     3301 cctccatctc ccagaacatt tattcggtga tccgcaatta cctgacgtac aaccattatt
     3361 tcacaaaatt caacgtcaat gcattagtag ggcatgaagc ccagacatcc tcttttgaga
     3421 attccggcgg ctcacggtcg aatttcccta ccaacaatat acaggctctt aacgccggcg
     3481 atgctaccac cgccaggaac tcgggcgata atggcctcgg ctcctcactg gaatcctatt
     3541 tcggccgtat caacttttcc tgggatgata aatacctcgt caccggtaac gtccgcaacg
     3601 acggttcttc gaatttcccg gtcaacaacc gttgggtcac cacttggtca ggggcattcg
     3661 cctggaagat caacaacgaa tcgtttttga aaggggtaag ggatataaac gaactgaaat
     3721 tgagactcgg atatggattg accaacaacc agggcatccc gggcaacacc tatgttaccc
     3781 aattacaaac ggtggccaat ggcctgtcgg gtatcgccca gtatcaaagc aacctggcaa
     3841 accccaatgt cacctgggaa aaaacgaact attacaacgg gggactcgat gcaacggtgc
     3901 taaacgggcg ggtgagtttt tcattggacg cctactaccg taaaacagac gggctcttat
     3961 tgaaggttcc gctcccgtta tactccggca ccaccgccgg ttattcgccg ggatcgatgg
     4021 cggcgcctta tgtcaatgtc ggcgccgtca gcaataaagg gttcgacttc agggtcagta
     4081 ccactaatgt caatggtagg aactttacct ggaaaaccga tgttaccgtc tcgcgtaatg
     4141 tcaataaagt actcaattta ggcgccggag accaggctaa cctgagtcaa aagtcctatg
     4201 tgatcaacga tattgtcgaa aagacggtgg tgggtcagcc cattggtgag ttttatggct
     4261 acatc
//
LOCUS       DATGFG010000398         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1277345, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000398 DATGFG010000000
VERSION     DATGFG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277345"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VKQ52_07510"
     CDS             <1..792
                     /locus_tag="VKQ52_07510"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HLZ87068.1"
                     /translation="ESNINKRLYRAKEKIRNDGIDFSVPTGAHLRLRLDAVLLVIYLL
                     FNEGYNSAGDNPAIRKDLCLEALRLAMLLTEKPATNEYPPVYALIALLCLHSARFDAR
                     LDNNDSLVILEDQDRTHWDRELIRQGLHFLSLSATGSTFTAYHLEAAIAAEHCLAPDF
                     ASTNWQRIHDYYTALEKLKPSSVIRLNLAIVTGKKDGPQAAIATLHELETHKALENYY
                     LLYASLGEFYFLLGRKEEALNYFLHAKTLTRSPAIRELIDRKMSR"
     gene            complement(854..2200)
                     /locus_tag="VKQ52_07515"
     CDS             complement(854..2200)
                     /locus_tag="VKQ52_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HLZ87069.1"
                     /translation="MKKRTFLKLSSVMMAGSALSPLMDWAQGEKITNWAGNLTYSTTS
                     LHKAVSIDDARAWVRSQPRFKVLGTRHCFNDIADSKDEFLSLTGLNKVIALDKVNHTV
                     TVESGIRYGELAVFLNDNGYALHNLASLPHISVAGGVATATHGSGAHNGNLSSAVMGM
                     EFLTAAGEPVSLTRRQHGEQFLAAVVHLGGIGVVTKVTLAIQPTYQVRQYVYENLPMA
                     QLEHHFDEIMSAGYSVSLFTDWRNRNVNEVWIKSRVGESGNFGTRPEFFGAHSATRNL
                     HPIRDISAENCTPQMGVPGPWHERLPHFKMGFTPSSGKELQSEFFVPREHSYAAIMAV
                     EKLHEQISPHLLITELRCIAADDLWMSPCRHQDSTAIHFTWKPEWEAVQKLLPQIEEA
                     LAPYRARPHWGKLFTMPHSRLAELYPKMREFQQVLRHYDPGGKFRNAYLNRVVFGV"
BASE COUNT          528 a          855 c          483 g          431 t
ORIGIN      
        1 gaatccaata tcaacaaacg cctctatcgc gcaaaggaaa agatccgcaa cgacggcatc
       61 gacttctccg tccccaccgg cgcccacctc cgcctccggc tcgacgccgt cctcctcgtc
      121 atctacctcc tcttcaacga aggctataac tccgccggcg ataatcccgc catccggaaa
      181 gacctctgcc tcgaagccct tcgcctcgcc atgctcctca ccgaaaaacc cgcgacaaac
      241 gaatacccgc ccgtctacgc cctcatcgcc ctgctctgcc ttcactccgc ccgcttcgac
      301 gcccgtctcg ataataacga cagcctcgtc atcctcgaag accaggaccg tacacactgg
      361 gatcgcgaac tgatccggca gggcctgcac ttcctctccc tgtccgccac cgggtccacc
      421 ttcaccgcct atcacctgga agccgccatc gccgccgaac actgcctggc acccgacttc
      481 gcctccacca actggcaacg catccacgac tactacaccg ccctcgaaaa actaaaaccc
      541 tcctccgtca tccgcctcaa cctcgcgatc gtaacaggca aaaaggatgg cccgcaggcg
      601 gcgatcgcca ccctgcacga actggaaacc cataaagcac tggaaaatta ttacctcctc
      661 tacgcctccc tcggcgaatt ctattttcta ttgggcagaa aagaagaggc cctcaactat
      721 ttcctccacg caaaaaccct caccaggtct cccgccatcc gcgaactgat cgatcgcaaa
      781 atgtcgcgct gaacggccac caacctccgc caccgtaaac tcccaccaac caccaacctc
      841 cgccaccgca aactcagact ccaaacacca ccctgttcaa atacgcattc ctgaacttcc
      901 cccccggatc ataatgccgc aacacctgct ggaactcccg catcttcgga tacagctccg
      961 ccaatcggct atgcggcatc gtaaacaact tcccccaatg cggtctcgcc cggtacggcg
     1021 ccagcgcctc ctcgatctgc ggcagcaact tctgcacagc ctcccactcc ggcttccagg
     1081 taaaatggat cgccgtactg tcctggtgcc ggcacggact catccacaga tcatccgccg
     1141 caatacaccg cagctcggtg atcagcagat gcggcgagat ctgctcatgc aacttctcca
     1201 ccgccatgat cgcggcataa ctatgctccc ggggaacaaa gaattccgac tgcagctcct
     1261 tcccgctgct cggcgtaaac cccatcttaa aatgcggcag ccgctcgtgc cagggaccgg
     1321 gcacccccat ctgcggcgta cagttctccg cggagatatc ccgtatcggg tgcaggttcc
     1381 tggtagccga atgcgcacca aagaactccg gcctcgtccc gaaattcccg ctctccccca
     1441 cccggctctt gatccacacc tcgttcacat tacgattgcg ccaatcggta aaaagactca
     1501 cactataccc cgccgacatg atctcgtcaa aatgatgctc cagctgcgcc atcggcaggt
     1561 tctcatacac atattgccgc acctggtagg tcggctgtat cgccagcgtc accttcgtga
     1621 ccaccccgat acccccaaga tgcaccacgg cggcaaggaa ctgctcgcca tgctgccgcc
     1681 gcgtcaggct caccggctcc cccgcggccg tcaggaactc catccccatc accgcactgg
     1741 acaggttgcc attgtgcgca cccgacccgt gcgtggccgt agcgacaccc cccgccacgg
     1801 aaatatgcgg cagcgaggcc aggttatgca gcgcatagcc attatcgttc aggaaaaccg
     1861 ccagttcgcc atagcggata ccggactcca ccgtcaccgt atgattcacc ttgtccagcg
     1921 cgatcacctt gttcagcccg gtgagcgaaa ggaactcatc cttcgaatcg gcaatatcat
     1981 tgaaacaatg ccgcgtaccc aacactttga atcgcggctg actccgcacc cacgcccgcg
     2041 catcatcgat cgacacggcc ttgtgaagcg aagtagtact ataggtaaga tttccggccc
     2101 agttggtgat cttctcaccc tgtgcccaat ccatcaacgg cgataacgcg gaaccagcca
     2161 tcataacgga agacagtttt aggaatgttc gtttcttcat acggcgcaat taggcaacca
     2221 agttaactaa ttttgccgat cttccgccgc cccttgacca gcaacctttg tccggaaaat
     2281 cgcgtatcgt aaaccat
//
LOCUS       DATGFG010000399         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1406665, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000399 DATGFG010000000
VERSION     DATGFG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406665"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1895
                     /locus_tag="VKQ52_07520"
     CDS             60..1895
                     /locus_tag="VKQ52_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87070.1"
                     /translation="MFKKIVAFLMPVVVLMTGCSKDPSQVLPTNLYSVADYPNTIDGL
                     NSVLATAYSAMRDANMFGFNFLPKAMANCTHLADDGGFDAGWTEMCKTDLTVANSYAQ
                     GVWAVCYAGIKNCNSVLTAANVYMTKYALPNDGPTVNLIRGQAYCLRGYYYIWLETLF
                     GQDFVPNGSAGLGVPMDTVLSASLEASQIGRSPIKDVWALIEGDLKQAASLLHGQVWT
                     GNDVGRASEWAAKGLLGKAYIYARDYANAKTTLSDVITNSGKTLMPYAKYRNAFTGIS
                     ANEFNEESLFELNIDYAANGNYGVYGPAPNSTSINGLIWSPYVLGTDGAETDAIALGY
                     GNEVIHDKNILRFGFPLGPYGLVSNPAYDPSKKASPSNPVLIMDPVYRQNSLLVRTAQ
                     TADPRLYVNCLQPWIDSAVNTPAAGYVPGTIPTAGFVPVSRPSGPTKANAYQWSFRKY
                     APIYSNVNNVPGGQADGANIYLLRLADVYLLYAEACINTNDGATGLEYLNKVKRRAYG
                     YPVDQPSAVDYASLTGATSAAAAGDPVLGHNPLYYERWAELFNEGQWWTDICRWHLGA
                     SEAAFYAADMASPALAFPDKSYAWPIPLQEVNANSKIAGQQNPGY"
     gene            2178..>3216
                     /locus_tag="VKQ52_07525"
     CDS             2178..>3216
                     /locus_tag="VKQ52_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HLZ87071.1"
                     /translation="MQLRFSLDREGEPVYSVNYGNKPVVLWSRLGFALSADSLFYKGF
                     SLRGSEQRAVDDRWKPVWGEVAAIRDHYQQLTVHLQKGGAAGLWLDIVFRVFEDGVGF
                     RYVFPKQAGLTYFIVTDERTEFHLAGDHKSFWIPGDYDTNEYPYTTSKLSEVDNRALV
                     AASTAIAVRVAPDPHAVQTPLMMKTADGLYLNIHEAGLAGYSAMQLHVDAGTLTLSAS
                     LVPDAVGNKAYLHAPAATPWRTIIVSDKAADILASKMILNLNEPSKLTVTDWIHPMKF
                     VGVWWEMQTGKGTWSYSDYADSVSAGGASIPNGRHSANTANVKRYIDFAAKNHIGGVL
                     VEGWNVGWEDWF"
BASE COUNT          621 a          686 c         1120 g          789 t
ORIGIN      
        1 cagacgagga tctattctgc cggggtagat tttaattttt aagctttaaa tttttactaa
       61 tgttcaagaa gattgttgcc tttcttatgc ctgtggtggt attgatgacc ggctgttcca
      121 aggacccttc acaggtgttg ccgaccaacc tgtattccgt ggcggactat cccaacacga
      181 tcgatgggtt gaacagtgtg ctcgccacgg cgtattcggc gatgcgggat gccaatatgt
      241 tcgggttcaa tttcctgccc aaggctatgg ccaattgtac gcatcttgcc gatgacggcg
      301 ggtttgatgc cggctggacg gagatgtgca agacggatct tacggttgcg aattcctatg
      361 cgcagggggt ctgggcggtt tgttatgccg ggatcaagaa ctgtaattcg gtgttgacgg
      421 cggcgaatgt atatatgacg aaatatgcgc tgccgaatga cgggccgacg gtgaacctga
      481 tccgggggca ggcctattgt ctgcggggct attattatat atggctggag accctgttcg
      541 ggcaggattt tgtgccgaac ggttcggctg gtcttggcgt gccgatggat acggtcttgt
      601 cggcgtcgct ggaggcctcg caaatcgggc gcagtccaat caaggatgtc tgggcgctga
      661 tcgaagggga tctgaagcag gcggcgagcc tgttgcatgg ccaggtgtgg accggcaacg
      721 atgtagggcg ggccagtgaa tgggcggcca agggtttgct gggtaaggcg tatatctatg
      781 ccagggacta tgcgaatgcg aagaccacgc tgtcggacgt gatcacgaac agcggcaaaa
      841 cgctgatgcc gtatgcgaaa taccggaacg cgtttaccgg gatcagcgcc aatgaattca
      901 atgaggagtc gttgttcgag ctgaacatcg actatgcggc caatggcaat tatggggtgt
      961 atggccccgc gccgaattcg acgagcatca atggactgat ctggagtccg tacgtgctgg
     1021 gaacggatgg ggcggagacg gatgcgattg cgctgggcta tgggaatgag gtgatccatg
     1081 acaagaatat cctgcggttt ggttttccgt tgggaccgta tggccttgtc tccaatccgg
     1141 cctatgatcc gtcgaagaag gcgtcgcctt cgaacccggt gttgatcatg gacccggtgt
     1201 accggcagaa ttcgttgctg gtgcggacgg cgcagacggc tgatccgcga ctgtatgtga
     1261 actgtcttca accctggatc gactcggcgg tgaatactcc tgcggcgggg tatgttccgg
     1321 ggacgatccc gacggcgggt tttgtcccgg tgtcgcggcc gagtgggcct acgaaggcaa
     1381 atgcttatca atggagtttc cggaagtatg cgcccattta tagtaatgtc aacaatgtgc
     1441 cgggtgggca ggcggatggg gcgaatatct acctgttgcg gctggcggat gtgtatttgt
     1501 tgtatgcgga ggcttgtatc aatacgaatg acggggcgac ggggttggag tatttgaaca
     1561 aggtgaagcg gcgggcctat gggtacccgg tggatcagcc ttcggcggtg gattatgcgt
     1621 cgctgaccgg tgcgacgagt gcggcggcgg cgggtgatcc ggtgctgggg cataatccgt
     1681 tgtattatga acggtgggcg gagttgttca atgaggggca gtggtggacg gatatctgtc
     1741 gttggcatct gggggcatcg gaggcggctt tttatgcagc ggatatggcg agtccggcgt
     1801 tggcgttccc ggataagagt tatgcgtggc cgataccgtt gcaggaggtg aatgcgaata
     1861 gtaagattgc ggggcagcag aatccggggt attagggagg gggggtatga ggttcgggcg
     1921 ccgggaggtg gcgtaactgc tgtggtgggg gtgtgggaaa tttggcctgt ttttgcggac
     1981 aatatgtata tttgacacat tacgatccgt gtgcgggatt gcggtgcagg tgccgggtgg
     2041 cgcacgggtc gttgtccttt caatgagaaa attggttcgt tatgccttat agggtaattg
     2101 cttgcttttg cttatgcttt ttttgttttt gctacacgat ccgcgcccag gagaaactaa
     2161 ccgccgcact tgggcgggtg caattgcgtt tttcgcttga ccgggagggg gagccggtgt
     2221 attccgtgaa ttatgggaac aagccggtgg ttttgtggtc gcggcttggg tttgcgctgt
     2281 cggccgactc gcttttttac aaaggttttt ccttgcgggg ttccgagcag agggcagtgg
     2341 atgaccgctg gaagccggtg tggggagagg tagcggcgat cagggatcat taccagcagc
     2401 tgacggtcca tttgcagaag gggggtgcag cggggttgtg gctggatatt gttttccgcg
     2461 tttttgagga tggggtcggg ttccgttatg tattcccgaa gcaggcgggg ctgacctatt
     2521 ttatcgttac ggatgaacgg acggaattcc atctggccgg cgaccataaa tccttttgga
     2581 tccccggtga ctatgatacg aacgagtatc cctatacgac atcgaaattg agtgaggtgg
     2641 ataaccgggc gcttgtggcc gcatctacgg ctatcgccgt gcgggtggcg cctgatccgc
     2701 atgcggtgca aacgccattg atgatgaaga cggcggatgg gctttacctg aatatccatg
     2761 aggcggggct ggcgggttat tccgccatgc agttgcatgt cgatgcgggg acgctgacgc
     2821 tctcggcttc gctggtgccg gatgcggtgg gtaacaaggc gtacctgcat gcgccggcgg
     2881 ccacgccgtg gcggacgata atcgtgagcg acaaggcggc ggatatcctc gcatcgaaga
     2941 tgatcctgaa tctgaatgag ccgtcgaagc tgacggttac ggactggatc cacccgatga
     3001 agtttgtggg ggtctggtgg gagatgcaga cgggcaaggg aacatggagt tattcggatt
     3061 atgcggacag tgtttccgcg ggtggggcgt cgattcccaa tggacggcat agcgccaata
     3121 cagccaatgt caaacgctat atcgattttg ccgcgaagaa ccatatcggg ggtgtgctgg
     3181 tggagggttg gaatgtgggt tgggaggatt ggtttg
//
LOCUS       DATGFG010000400         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1277246, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000400 DATGFG010000000
VERSION     DATGFG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277246"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VKQ52_07530"
     CDS             complement(<1..417)
                     /locus_tag="VKQ52_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLZ87072.1"
                     /translation="MRVGLFIPCYVDQFYPQVGIATLELLEKFGCEVVYPPDQTCCGQ
                     PMANSGFEHLTGGCTTLFIKNFNDFDYIVSPSGSCVLHIKEHSHEDAGLHNRIFELVE
                     FLTDILKIDDLDASFPYRVGLHQSCHGQRGLHLAQMT"
     gene            complement(423..1760)
                     /locus_tag="VKQ52_07535"
     CDS             complement(423..1760)
                     /locus_tag="VKQ52_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HLZ87073.1"
                     /translation="MIAIFDIGKTNKKLFLFDEQYQIVWERSCICEQTVDEDGEPCED
                     LAQLNAFIRDSLAQLPNDLTVRAINFSAYGASFVYIGKEGKPVAPLYNYLKPFPDDLR
                     KRFYGAHGGEVTFSMLTASPVLGSLNSGMQLYRLKEEQPKLFEKISYALHLPQYLSYL
                     LTGAPCSDITSIGCHTNLWNFSQQHYHEWVYREGIIDKLAPILPSTSVLPVAGASAIP
                     PAATGNAAAIPTGSRIPVSGIGLHDSSAAMIPYFESFREPFLLISTGTWCISMNPFNH
                     SPLTVAELQKDCLCYMSYQGKPVKASRLFAGYEHEQRVQQLAARFNVKPAAAAVVMYD
                     PAIIRQLSSDPRPEPATFEQAYHHLLMDIMEKQVASTQLVLHDTDVKRIFVDGGFGKN
                     QVYMHLLAEAFPDIEVFAASIPQATAMGAALAIHEHWNSRSRPTDIIELKYYK"
     gene            complement(1757..>2371)
                     /locus_tag="VKQ52_07540"
     CDS             complement(1757..>2371)
                     /locus_tag="VKQ52_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87074.1"
                     /translation="FYSMTVGDWGQSLLYATKLGPKAYTLVDLGHHLPNANIEQIVAL
                     LLMEGKLGGFHFNDSKYGDDDLTVGSIKPYQLFLIFNELVEGMDARGMDHTRDLGWMI
                     DASHNVKDPLEDLLQSVEAILITYAQALALDRKKLIAAQQANDTVAAQEVVQNSFRTD
                     TRALVAEARLRAGAALDPLALYRAENVREHLTKERGAGAVATGL"
BASE COUNT          470 a          562 c          792 g          547 t
ORIGIN      
        1 cgtcatctgc gccaggtgca acccccgttg tccgtgacag ctttggtgga ggccgacccg
       61 ataggggaag ctggcgtcga gatcgtcgat cttcaggatg tcggtgagga actcgacgag
      121 ttcgaatatc ctgttatgaa ggccggcgtc ttcgtgggaa tgttccttga tatgcaggac
      181 gcagctgccg gatggcgaaa cgatataatc gaaatcgttg aaatttttta tgaagagagt
      241 ggtgcagccg ccggtgaggt gttcaaaacc ggagttggcc atgggttggc cacaacaggt
      301 ctgatcgggg ggatagacta cttcgcaacc aaatttttcc agcaattcca gggtggcgat
      361 gcccacctgc ggataaaact ggtcgacata gcagggaatg aacaaaccta cacgcatgag
      421 tcttacttgt aatatttcaa ttctataata tccgttggcc gggagcggga gttccagtgt
      481 tcgtggatcg ccagcgccgc acccatggcc gttgcctgtg ggatggacgc cgcgaagact
      541 tcgatatccg gaaaggcttc cgccagcagg tgcatgtaga cctggttctt cccaaagccg
      601 ccgtccacga agatacgctt gacatcggta tcatggagca ctaattgcgt ggaagcgact
      661 tgtttttcca tgatgtccat caggaggtgg tgataggcct gttcgaaggt ggcgggttcg
      721 ggccggggat cggagctgag ctgccggatg atagcggggt cgtacataac gacggcggcc
      781 gcggcgggtt ttacgttaaa ccgggcggcc agctgttgaa cgcgctgttc gtgctcatag
      841 ccggcgaaga gacgggaggc cttgactggt tttccctgat agctcatata gcagaggcaa
      901 tccttctgca attcggccac ggtgaggggt gagtggttga aggggttcat cgaaatacac
      961 catgttcccg tggaaataag caggaagggc tcgcggaagc tttcgaaata ggggatcatg
     1021 gccgcggagc tgtcgtggag gccgatgccg ctgacgggga tgcgggatcc ggttggaatt
     1081 gccgcggcat tgcccgttgc ggcgggcggg atcgccgagg cgccagctac aggcaggacg
     1141 cttgtcgacg ggaggatggg cgccagtttg tcgatgatgc cttcccggta gacccattcg
     1201 tggtaatgct gttgcgagaa attccagaga ttggtatggc agccgatgct ggtgatatcg
     1261 gagcaggggg cgccggtgag caggtagctg aggtattggg gcaggtggag ggcgtaggat
     1321 atcttttcga acagctttgg ttgctcttcc tttagccggt agagttgcat cccggaattg
     1381 aggctcccca gtacgggaga ggcggtgagc atggaaaatg tgacctcgcc gccatgggcg
     1441 ccgtagaatc tcttccggag atcgtcggga aagggtttta ggtaattgta cagcggggcg
     1501 acgggtttcc cttcctttcc gatatacacg aagctggcgc cataggcgga gaaattgatg
     1561 gcgcggacgg tgaggtcgtt gggcagttgg gcgagggagt cccggatgaa agcgttgagc
     1621 tgcgccaaat cctcgcaggg ctccccgtct tcgtcgaccg tttgttcaca gatgcagctc
     1681 cgttcccaga cgatctggta ttgttcgtcg aagaggaaga gttttttatt ggtcttgccg
     1741 atgtcgaaga tggcgatcat aatccagtag caacggcgcc ggcgcctctt tcttttgtga
     1801 ggtgttcgcg tacgttttca gcgcgataga gcgccagggg gtcgagggcg gcgccggccc
     1861 gcagccgggc ttcggccacc aacgcacggg tgtcggtacg gaaactgttt tgtactactt
     1921 cctgggcggc gacggtatcg tttgcctgct gggcggcgat gagctttttg cggtcgaggg
     1981 ccagggcctg tgcataggtg atcaggatgg cctcaacgga ttgcaggagg tcttccagcg
     2041 ggtcttttac gttgtgagag gcgtcgatca tccatccaag gtcgcgggta tggtccatac
     2101 cccgtgcatc catgccttcc acgagttcgt tgaagatgag gaagagctgg tagggcttga
     2161 tacttcccac cgtcaggtca tcgtcaccgt atttggagtc gttgaagtgg aaaccgccga
     2221 gttttccttc catcaggagc agggcgacta tttgttcgat gttggcgttg ggcaagtggt
     2281 ggccgaggtc gacgagggta taggcctttg gcccgagctt tgtcgcgtac agcagggatt
     2341 gaccccagtc gcccactgtc atagagtaga a
//
LOCUS       DATGFG010000401         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_251289, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000401 DATGFG010000000
VERSION     DATGFG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251289"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3211
                     /locus_tag="VKQ52_07545"
     CDS             <1..>3211
                     /locus_tag="VKQ52_07545"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HLZ87075.1"
                     /translation="KGQWDPRVQFKGELPTGALFLEKKGFSAVLYNPEDLMRLTEAHH
                     GIKGAGSAKPGGSGGSGGGGIAKMAASTGSVLKTGGPGGVPAADQLRAHAYRVSFADA
                     NEDVTIVPDKILPGYNNYFIGNDPSKWGSNCRVFQGVTYQNLYPNIDVRYYTNEGQLK
                     YDIIVHPGGDVRRIRMRYEGADRLSIRKNQLVVGTSVGAVTQLSPYSYLYNQQGRTDI
                     RCRYVIRNGNMVSFDLPDHDSSATLIIDPTVIFCSFSGSKVSNWGFTATPGPDGSFFF
                     GGIVFGSAFPFTTGALEPSYSGGAFDVGLLKLNASGTSKVYATYLGGNDSETPHSLIC
                     DAQGNLIVLGRTYSSTFPFLTQEGSGGGADMFVAKINASGSKLIGCMRIGGSSDDCVN
                     MEDQARTHYEKADSLIRNYGDDSRSEVVLDGQNNILIAGSSQSRDFPITGTVFQPNFG
                     GGRQDGVVLKIDPTCNKLIWASFLGGVGADAAFVLKTNPMTGDIYVAGATTSTDFPGA
                     ANGGVIQGAYSGGVSDGYVAQISADGSRLIRSTYLGTSAGDAIYGLQFDKKGFPYVMG
                     TTNGTWPVLNVNYSIGGGRQFVSKLQPDLSAYIYSTTFGTPSGLSRGMPNISPVAFLI
                     DRCENIYVSGWGGWIIGGETDPYGLAGTAGLPTTPDAYKRLSDNRDFYFIVLSKNANG
                     LLYGTFFGENDNSQSISEHVDGGTSRYDQYGVIYQAICANCSGPQMTGRYPTTPGVWA
                     PVNGAGNSGCNMAAIKIAFNFSGVAGGMRISVNGRTGDTSGCIPMDTYIQDTIRNAKQ
                     YIFNFGDGSPDTATTSYAVSHTYVNRGTYTIMMVAIDSNSCNVADTTYRHVIARTDRA
                     PVSFTVTKDPNAPCTSLDYDFTNTSLAPVGKPFGPTSFVWQFGDGSPPRTMGFGPTNY
                     SYSGPGTYIVNLILDDTSYCNYPDTATEMLRVSPVAKAQFVTPSTGCAPYAAVFNNTS
                     LGGTSFTWNFGDPGSGELNTSSDVNPTHVYQTPGTYTISLHEEDPNTCNKTSDTSFTI
                     TVSVRPKAAFTYAPSPPVANVPIVFTNGSQGGVSYLWKFG"
BASE COUNT          633 a          789 c         1033 g          756 t
ORIGIN      
        1 acaaaggcca gtgggacccc cgtgttcaat tcaaggggga gctgcctacg ggtgcgctat
       61 tcctggagaa gaagggtttt tcggccgttc tgtataaccc cgaggacctg atgcggctga
      121 cggaggcgca tcatgggatt aagggggcgg gttcggccaa gccgggaggt tccgggggta
      181 gtggcggggg tgggatcgcg aagatggccg cttccaccgg gtctgtgttg aagaccggcg
      241 gcccgggtgg cgtaccggcg gcggatcagc tgcgggcgca tgcttaccgg gtgagttttg
      301 ccgatgccaa tgaggatgtc accattgtcc cggataagat cctgccgggg tataacaact
      361 attttatcgg gaacgaccct tcgaaatggg gctcgaactg cagggtcttc cagggggtga
      421 cctaccagaa cctttacccg aatatcgatg tccgttatta taccaatgag gggcagttga
      481 aatatgatat cattgtccac cccgggggtg atgtccggcg tatccgaatg cggtacgagg
      541 gcgccgaccg gctgagtatc cggaagaacc agttggtggt ggggacttcg gtaggggcgg
      601 tgacgcagct ttctccgtat tcttatctct ataaccagca ggggcgaacg gatatccgtt
      661 gccggtatgt catccggaac gggaatatgg tgagtttcga cctgccggac catgacagca
      721 gcgccacgct gatcatcgac cccacggtga tcttttgttc gttctccggc agcaaggtga
      781 gcaactgggg gtttacggcc acgccggggc cggatggcag cttttttttc ggggggatcg
      841 tctttggttc ggcctttcct tttaccacgg gtgcgcttga gccttcctat tccggcgggg
      901 cctttgatgt ggggttgtta aaactcaatg ccagcggcac cagtaaggtg tatgccacct
      961 atctcggggg gaacgattcg gagacgccgc acagcctgat ctgtgacgcg cagggcaacc
     1021 tgatcgtcct ggggaggacc tattccagta cttttccctt tctcacccag gagggcagcg
     1081 gcggcggagc cgatatgttc gtggcgaaga tcaatgcatc gggtagtaag cttatcgggt
     1141 gtatgcggat cggcggctcg tcagatgact gtgtgaatat ggaggatcag gcgaggaccc
     1201 attatgagaa ggccgattcc ctgatccgca attatgggga tgattcccgg agtgaggtgg
     1261 tgctggatgg gcagaataat atcctcatcg ccgggtcatc gcaaagccgg gatttcccta
     1321 tcaccggtac cgtcttccag cccaatttcg gcggcggccg gcaggatggg gttgtgttga
     1381 agatagatcc cacctgtaac aagctcatct gggccagttt tcttggcggc gtgggggcgg
     1441 atgccgcttt tgtgctcaag accaacccga tgacgggcga tatttatgta gccggcgcta
     1501 cgaccagcac tgatttcccc ggcgcggcca atggcggggt tattcagggg gcgtatagcg
     1561 gaggggtctc cgacgggtat gtggcccaga tatcggcgga cggttcgagg ctgatccgga
     1621 gcacttacct ggggacgagt gccggtgacg cgatctacgg attgcagttt gacaaaaagg
     1681 gttttccgta tgtgatgggg accacgaacg gcacctggcc ggtgcttaat gtaaactatt
     1741 ctatcggcgg ggggcggcag tttgtttcca agctgcaacc cgatctttcg gcctatatct
     1801 attccaccac gtttggcact ccgtcggggc tgtcgcgtgg tatgccgaat atctcgcctg
     1861 ttgcctttct gatcgatcgt tgtgagaata tctatgtaag cggctggggt gggtggatca
     1921 tcgggggtga gaccgatccg tacgggctgg cggggacggc ggggctgccg accacgccgg
     1981 atgcgtataa gcggcttagc gacaaccggg atttttattt tattgtacta tccaagaatg
     2041 cgaatgggtt gttgtatggg accttttttg gggagaatga caattcgcag agcatcagcg
     2101 aacatgtgga tggggggacg agccggtatg accagtatgg ggttatctac caggcgattt
     2161 gcgccaattg cagtggtccg cagatgaccg gccgttatcc gacgacaccg ggtgtttggg
     2221 cgccggtgaa tggggccggg aatagtggtt gtaatatggc tgcgataaag atcgccttta
     2281 atttttccgg ggttgcgggc gggatgcgga tctcggtgaa cgggcggacg ggggatactt
     2341 cgggttgtat cccgatggat acgtatatcc aggatacgat ccgcaatgcc aagcagtata
     2401 tcttcaactt tggggatggc agcccggata cggccacgac cagttatgcc gtgagtcata
     2461 cctatgttaa cagggggacc tataccatca tgatggtggc catcgattcc aacagctgta
     2521 atgtcgccga tactacttat cgtcatgtca ttgcccggac ggaccgggcg cccgtgagtt
     2581 ttaccgttac gaaggatccg aatgcgccgt gtacctcgct ggactatgat tttaccaaca
     2641 cttcccttgc gccggtgggt aagccttttg ggccgacttc cttcgtctgg caatttgggg
     2701 atggttcgcc gccgaggacc atggggtttg ggccgacgaa ctatagttat agcgggccgg
     2761 gtacttatat cgtgaacctg atcctggatg atacatcgta ttgtaattac ccggatacgg
     2821 cgacggagat gctgcgggta tcgccggtag caaaggcgca atttgtgacc ccatcgacgg
     2881 gttgcgcgcc ttatgcggct gttttcaaca acacctcgct tgggggcacc agctttacct
     2941 ggaattttgg cgatccgggc agtggagagc tcaacacgtc cagcgacgtc aaccccacgc
     3001 atgtgtatca gaccccggga acgtatacga tcagcttgca tgaggaggat cccaacacct
     3061 gtaacaagac ctcggatacg agctttacca ttacggtaag tgtccggccg aaggctgcct
     3121 ttacgtatgc gccttcgccg ccggtggcga atgtgccgat cgtctttacc aatgggtcgc
     3181 agggtggcgt cagttatctg tggaagtttg g
//
LOCUS       DATGFG010000402         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_549340, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000402 DATGFG010000000
VERSION     DATGFG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549340"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="VKQ52_07550"
     CDS             <1..1574
                     /locus_tag="VKQ52_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87076.1"
                     /translation="RALFVPFDNDMWVRYNAAQVKSADFTGSEVAALYNDSTRQGMVI
                     GSLDQDTWKTGIRIKGETDTTLSNLTVFAGFTDSVTTHDRIAHGTVHPVNGVCRGSKI
                     VIGDFDDWRTGMETYAHLDSLAQPKSIFNWNRPTPMGWNSWGTIQDKLTLSKATSVVD
                     FFADSCRGFRNGEHTLFIDLDAFWDNMTPGGVLGDVSKLDSFVTYCHRKGFRPGIYWT
                     PFAEWGKTDRKIEGSSHNYADTWTRQQGKLMDVDGGRAMDPTHPGTRERIVATLTRLK
                     QLGFEMIKIDFLGHGALEADHFYDTTVTTGMQAFRKGMAFVDSVLGGQMLVYSAISPN
                     IATARYVHMRRIGCDAFSAIDNTEYTLNSTGYGWWQSGIYNFVDADHVVFTNAPEGMN
                     RARLVSSLVTGTLMTGDDYSAPGKWRATARQLLQNPHLLAVVGKGKAWRPVFANTGNR
                     GVPVLVKHTGSAVYVAVINYENTPREFSIPISGIGAQVRKAKAIFGGQTVQSDGGQLK
                     VQVAGNDAEIFVKIF"
     gene            1689..>2855
                     /locus_tag="VKQ52_07555"
     CDS             1689..>2855
                     /locus_tag="VKQ52_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ87077.1"
                     /translation="MKKKLGYRICLLLALFAFSRAFSQQPANTPRPRITITGTVIDTT
                     GQRVVGASVTVPGKKSIGTSTDVNGRFILDVEEGATIIISYVGYAEQRITATSARKSV
                     DIVLRTAAAGESVVVTAYGKKVRKEAVVGSVTSVNPDALRIPASNLTNALAGQVAGVI
                     SFQRGGQPGADNSQFFIRGVTTLGYSASPLILVDNVELTANDLARLNVDDIASFSILK
                     DASAAALYGARGANGVILVTTKEGKIGKARINFRYENSVSKPTKNIELADPVTYMKGY
                     NEALTTRGGAPYWSPNQIQDATATWNHTPGSNPYLHPATDWMSMLFKKQTTTQRGNFS
                     VQGGNDLARYYISGSYDRDNGILQSNPVNNFNTGMKYENYQLRSNINVKVTKTTE"
BASE COUNT          696 a          769 c          818 g          572 t
ORIGIN      
        1 atcgtgcttt gttcgtgcct ttcgacaacg acatgtgggt tcggtataac gcggcacagg
       61 taaaaagcgc ggacttcacc ggtagcgagg tagccgcttt gtacaatgac tccacccggc
      121 agggcatggt catcggcagc ctggaccagg acacctggaa gaccggcata aggataaaag
      181 gagagaccga cacgacgctg tcgaacctca ccgtctttgc aggcttcacg gattccgtca
      241 ccacccatga ccgcatcgcg catgggacgg tgcatcccgt gaacggcgtg tgccgcggca
      301 gtaaaatagt gatcggcgat ttcgacgact ggcgcacagg catggagacc tatgcacacc
      361 tggacagcct cgcacagcca aagtccatct tcaactggaa caggccgacc ccgatgggct
      421 ggaacagctg ggggacgatc caggataaac tcacgctttc aaaagcaacg tcggtggtcg
      481 atttcttcgc ggattcctgc aggggattcc gcaacgggga gcatacgcta ttcatcgacc
      541 tcgacgcttt ctgggacaat atgacgcccg gcggggtgtt gggggacgtc tcgaagctgg
      601 acagctttgt cacgtactgt caccggaagg gcttccggcc cgggatctat tggacccctt
      661 ttgccgagtg ggggaagacg gaccggaaga tagagggttc gagtcacaat tatgcggaca
      721 cctggacaag gcagcaggga aagctgatgg atgtggatgg cggcagggca atggacccca
      781 cccatcccgg gacaagagaa cgcatagtcg ctaccctcac gcggttgaaa caattgggtt
      841 tcgaaatgat caagatagat ttcctggggc atggcgcgct cgaggccgat catttctatg
      901 acaccaccgt caccacgggg atgcaggctt tccgcaaggg aatggcattc gtcgacagcg
      961 tgctgggcgg ccagatgctg gtctattcgg ccatctcgcc caatatcgct accgcgcgtt
     1021 atgtccacat gcggcgcatc ggctgtgacg ccttcagcgc catcgacaac actgaatata
     1081 ccttgaacag caccggctac ggctggtggc aaagcggtat ctacaatttt gtggatgccg
     1141 atcatgtcgt gtttaccaac gcgccggagg gtatgaacag ggcgagactg gtgtcttccc
     1201 ttgtgacggg cacgctgatg acgggcgatg actattccgc ccccggcaag tggcgggcta
     1261 cggcgcgtca gctgttgcag aacccccacc tgctggcggt ggtgggaaag ggcaaggcct
     1321 ggcgtcccgt gtttgccaac accggcaacc gtggcgtacc cgtgttggtg aaacacacag
     1381 gttcggcagt gtatgtcgcg gtcatcaatt atgaaaatac cccccgggaa tttagcattc
     1441 ccatttcggg catcggtgcg caggtacgga aagcgaaagc catcttcggc ggacaaacag
     1501 tacaatcgga tggcggtcaa ttgaaagtgc aggtggccgg aaatgacgcg gagatttttg
     1561 ttaaaatatt ttgaaaaggc aggggaatgt ctatatttgt agaatagtat agtatagtac
     1621 agaaagttca cgccatctat ctataagggt ttttgctctg attaacttaa ccaaaacgat
     1681 gcttccgtat gaagaaaaaa ctaggctata gaatatgcct gctgcttgct ctatttgcct
     1741 tctccagggc attctcccaa caaccggcta ataccccgcg cccgcgcatc acgatcaccg
     1801 ggaccgttat cgacacgacg ggccagcggg tggtaggggc cagtgttacc gtcccgggta
     1861 agaaaagcat cggtacgagc accgatgtca acggccggtt catcctggac gtggaagaag
     1921 gcgccaccat catcatctcc tatgtagggt atgccgaaca acggatcacg gcgacatccg
     1981 cgcgtaagtc ggtggacatc gtcctgcgga cggccgcggc aggcgaatcc gtcgtcgtca
     2041 ccgcctatgg taagaaagtg cggaaggagg ccgttgtcgg ctccgtgacc agcgtgaatc
     2101 ccgatgcact tcggatccca gccagcaacc tcaccaatgc gctagccgga caggtggccg
     2161 gggttatctc ttttcaacgc ggcgggcagc cgggagccga taattctcaa ttcttcatcc
     2221 gcggagtgac taccttggga tatagcgcca gtccgctgat cctggtagac aatgtcgagc
     2281 tgacagccaa tgacctggcc cgcctgaacg tggatgatat cgccagcttc tccatactga
     2341 aagatgcaag cgccgcggca ttatacggcg caaggggcgc gaacggcgtt atccttgtca
     2401 ccaccaaaga aggaaagatc ggaaaagcca ggatcaattt ccgctatgag aattccgttt
     2461 ccaaaccgac gaagaatatc gagctggccg acccggtcac ttacatgaag ggttacaatg
     2521 aggccctgac gaccaggggt ggggcgcctt actggtcgcc taaccagata caggatgcaa
     2581 ctgccacctg gaaccataca ccgggcagca acccttattt acatccggcg acagactgga
     2641 tgtcgatgct gtttaaaaaa cagaccacta cccagcgggg aaactttagc gtacagggcg
     2701 gtaatgacct ggcccgttat tatatctccg gatcctatga cagggacaat gggatcttac
     2761 agtcaaaccc ggtgaacaat ttcaacaccg ggatgaaata tgagaattac cagctgcggt
     2821 ccaacatcaa cgtaaaggtg acaaaaacca cggaa
//
LOCUS       DATGFG010000403         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_9382, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000403 DATGFG010000000
VERSION     DATGFG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9382"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1268)
                     /locus_tag="VKQ52_07560"
     CDS             complement(141..1268)
                     /locus_tag="VKQ52_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87078.1"
                     /translation="MIQRLLQSVINDRMGKQKAILLLGPRQVGKTTLVKQLTSTMEGK
                     IGWFNGDEADTRQLLQNTTSTRLKALIGNNSVVVVDEAQRIPDIGITLKLIVDNMPEI
                     QIIATGSSALELAGQIKEPLTGRKYEYHLYPLSFAELVQHTSLLEEKRLLEHRLIYGY
                     YPEVVNKPGEERDTLQLLTDSYLYKDLFTLEQLKKPLLLEKLVQALALQLGNEVNYHE
                     LGQIVGADKETIERYVDLLEKAFVVFRLPSLSRNLRNEIKKGRKIYFWDNGVRNAVIK
                     NFNQLAQRQDTGALWENFIVTERQKINHYTGAYVNRYFWRTHAQQEVDYIEEHSGKLT
                     AWEFKWNPKAKGKIPKTFLEAYPGTETSIITPENMEDFVTG"
     gene            complement(1397..1777)
                     /locus_tag="VKQ52_07565"
     CDS             complement(1397..1777)
                     /locus_tag="VKQ52_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87079.1"
                     /translation="MMKKEISVVLVDDHIHFRNELATLIASFAEFVVPWQADNGKQLI
                     TRLAHGVVPDIVLLDINMPEMDGFETAAWLRTHYPAVKVLAISTYDNEKTINRMLSLG
                     ARGFVLKDVEPVEIRAALLDVAEK"
     gene            complement(1774..>2517)
                     /locus_tag="VKQ52_07570"
     CDS             complement(1774..>2517)
                     /locus_tag="VKQ52_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87080.1"
                     /translation="VRINKLQQEFQHAILSSKLEMQEQTFQEISQEIHDNIGQILSLA
                     KLHIATMDAAGSPVLENKIRDSKALVSKAIGDLRNLSYRLNTDYITDVGFDNAVKREI
                     ETIRRSEAYEARFSIEGKPYRFDQQKELILFRIVQELLNNILKHARAKTITVELHYSP
                     TALLVTIRDDGQGFDRSALGTPATNGNGSDSEGHGLGIKSMYHRARLIGAEFQLTSAI
                     GAGTVAAIHIPFNPTIDSLPLQPAKNSTL"
BASE COUNT          538 a          627 c          665 g          687 t
ORIGIN      
        1 caggctttcg gcggtgtcga ggacggtctg gacttcgggg gaaaaaaggg aggtgaacat
       61 atcccttaag ttagggattg tttggatgcg ctgttaccgt ataaacacct cttttttgtc
      121 tttggaacat caaggccttc ctatcccgtt acaaaatctt ccatgttctc aggggtgatg
      181 atacttgttt cagtacctgg ataagcctcg aggaatgttt ttggtatctt tccctttgct
      241 tttggattcc acttgaattc ccatgctgtc aacttgccgc tatgttcttc aatataatcc
      301 acttcctgct gggcatgagt gcgccagaaa tatctgttca cataggctcc ggtgtaatga
      361 ttgatctttt gccgctcagt tacgatgaaa ttttcccaaa gtgcgccagt atcctggcgc
      421 tgcgcgagtt ggttgaaatt tttgatgact gcattgcgaa cgccgttgtc ccaaaaatat
      481 attttccggc cttttttgat ctcgttacgc aggttgcggc taagcgaggg aagacggaaa
      541 acgacaaacg ccttttccag cagatcgaca taacgttcta tcgtttcctt atccgcgccg
      601 acgatttgac ctaactcatg ataattcact tcattaccta attgaagggc aagcgcctga
      661 acgagctttt ccagcagcaa tggctttttt aactgttcca gggtgaacag atccttgtaa
      721 aggtaactgt cggtaagtaa ctggagcgta tctctttcct ctccgggctt gttcaccact
      781 tcaggataat aaccgtaaat caggcgatgt tcaagcaatc gcttctcctc cagcaggctg
      841 gtatgttgta ccagttccgc aaaactcagg ggataaaggt ggtattcata tttgcgaccc
      901 gttaacggct cttttatttg tcctgccaac tccaatgcag aagaccctgt cgcaataatt
      961 tgtatctccg gcatattatc cactatcagt ttgagtgtaa tgcctatatc cggaatacgc
     1021 tgagcttcat ccacaactac aacagaatta ttaccgatca gcgccttgag acgcgtcgaa
     1081 gtggtattct gcaacaattg acgggtatca gcctcatcgc cgttaaacca tcctattttt
     1141 ccttccatag tgctggttag ttgctttacg agggtagtct tgcctacctg tcgggggcca
     1201 agaagaagga tggccttttg cttccccatc cggtcattta ttacggattg cagcaatctt
     1261 tggatcatag gcaaatttat gaatttgtcg tattaaaacg aaaaaataat attaaaattt
     1321 cgttttaaac cgcaaattta gccagaaacc ggtcgcggcg cttgcgaagc aggcctcaac
     1381 cgggggctcg gggaacctac ttctccgcca catccagcag cgcggcgcgg atctccaccg
     1441 gctcgacatc cttcagcacg aagccgcggg caccgaggct cagcatgcgg ttgatcgtct
     1501 tttcgttatc gtaggtggag atggccagca cctttaccgc ggggtaatgg gtccggagcc
     1561 aggcggcggt ctcgaagccg tccatctcgg gcatattgat gtcgagcagc acgatgtcgg
     1621 ggacgacgcc atgggcgaga cgggtgatga gttgtttgcc gttatcggcc tgccagggca
     1681 cgacgaattc ggcgaagctg gctatcagtg tggcaagctc gtttcggaaa tggatgtggt
     1741 catcgaccaa tacaacggag atctctttct tcatcatagt gtactgtttt ttgccggttg
     1801 caatggtaag ctgtctatcg ttgggttgaa tggaatgtgg atggcggcta ctgtgccggc
     1861 accgatggcg cttgtcagct ggaattcggc gccgatgagc cgggcgcgat ggtacatgct
     1921 ttttatgccg aggccgtggc cctcgctgtc gctaccattc ccatttgtgg ccggcgtccc
     1981 gagggccgac cggtcgaagc cctggccatc gtcccggatc gtgacgagca gggcagtggg
     2041 cgagtaatgg agctcgacgg tgatggtctt cgccctggcg tgtttcagga tattgttgag
     2101 cagctcctgt acgatgcgaa agaggatcag ctccttttgc tgatcgaacc ggtagggttt
     2161 gccttcgatc gagaaccgcg cctcgtaggc ctccgaccgc cggatggtct cgatctcgcg
     2221 tttgacggca ttgtcgaagc ccacgtcggt gatatagtcg gtgttgagcc ggtaggaaag
     2281 attgcgcaag tcgccgatag ccttcgacac cagcgccttc gaatcccgga tcttgttctc
     2341 cagcacaggg ctgcccgcgg catccatcgt cgcgatatgg agcttggcga gactgaggat
     2401 ctgaccgata ttatcgtgaa tttcctggct gatctcctgg aaggtctgct cctgcatctc
     2461 cagtttggag ctaaggatgg cgtgctggaa ttcctgttgc agcttgttga tccgcac
//
LOCUS       DATGFG010000404         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_7130, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000404 DATGFG010000000
VERSION     DATGFG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7130"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="VKQ52_07575"
     CDS             complement(<1..1662)
                     /locus_tag="VKQ52_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ87081.1"
                     /translation="MLHRPRIILLLRVILHRVRIILLLLLAAGCKPGQDNRLFTRLDK
                     DATGIDFQNTLFDGEALNVLNYIYFYNGGGVAIGDINNDGLPDILFTGNMVHNRLFLN
                     KGNFTFEDITEKSGLAAPSVEGWATGATMVDINGDGKMDIYICRSADANPARRRNLLF
                     INNGPASGADPHAAPTFSEKAAEYGLADEGFSTQAAFFDYDKDGDLDCFLINHSLHQY
                     TTGAIENPGWRKESRPAFECKLLRNDWNDSLHHPVYTDVSAQAGIHSDVLTFGLGLAI
                     SDLNNDGWPDIYVSNDFNEPDYLFLNNGPSHTFTESLANCMDHTSLYSMGNDAADFNN
                     DGLVDLMTLDMLPEDNHTQKMHSGAENFNKFQQLFNHGFYYQYSRNMLQKNNGDGTFS
                     EIGQLAGVSNTNWSWSALFSDFDNDGNKDLLITNGYVRDYTDMDFLKYSADNAMHNNT
                     TDKEAAIKDAIAKMPQSPDKSYLFRNNGNASPQAAGDGHDGPVFTRVNKEWGLDQQTV
                     SAGAAYVDLDNDGALDLVINNTNEYAGIYRNNARSLPNHHYIKIRL"
     gene            complement(1721..>3130)
                     /locus_tag="VKQ52_07580"
     CDS             complement(1721..>3130)
                     /locus_tag="VKQ52_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ87082.1"
                     /translation="NLFADQYAQYFACEATYFGTDRLVINQAWVGANFNPYYTDVLPQ
                     LQTIFANTDSAKPEHAMAEVVWVLTFMKATDYWGPIPYFQAGTVATSVPYDAQDKIYA
                     DFFHRLDGAIAILKANAGGNAYGSYDLIYGGSVDKWRKFANTLRLRLALRVSKADPAT
                     AKTEAEASVADGVMTTSPDDDGLIKRSSVGGDINGLSTMSDWNEFRMSATMASVLKGY
                     QDPRISQYFLPTVNSGDPTFGKFANYQGLRNGLSVAQLTLSPNLAAANSHQGQRWSST
                     SVTVGGTVVGEASYTNTPQHVIETAEAYFLRAEGALLGWNMGGTAQSLYETGIKNSMA
                     QWGITDATTVNTYIASANTPIAPGDYLNSPAVSTVPIAWSADPSVELQQIMTQKWLAL
                     FPDGMEAWADWRRSHVMKLYPAANSDNQLIPDPSKLWLRRIPFLLTERQNNAAGVQTG
                     VTALGGTDNELTPLWWDKN"
BASE COUNT          538 a          693 c         1150 g          749 t
ORIGIN      
        1 gaggcggatc ttgatatagt gatggttcgg caagctgcgg gcgttgttgc ggtagatgcc
       61 ggcgtattcg tttgtgttgt tgataaccag gtcgagggcg ccgtcgttgt cgagatcgac
      121 gtaggctgcg ccggcggaga cggtctgctg gtcgaggccc cattctttat tgacgcgggt
      181 gaatacaggc ccatcatgtc cgtcgccggc agcctgcggg gaggcgttac cgttgttacg
      241 gaagaggtag cttttgtcgg ggctttgcgg cattttggcg atcgcgtctt ttatggcggc
      301 ttccttgtcg gtggtgttgt tgtgcatggc attgtcggca gagtatttga ggaagtccat
      361 gtcggtatag tcccggacgt agccgtttgt gataaggagg tctttgttgc cgtcgttgtc
      421 gaagtcggag aagagggccg accaggacca gttggtgttg gagacgccgg ccagctggcc
      481 tatttcgctg aaggttccgt cgccgttgtt cttttggagc atattgcggc tgtactggta
      541 gtaaaagccg tggttgaaga gttgttggaa cttgttgaag ttttccgcgc cgctgtgcat
      601 tttttgggtg tggttgtctt cgggcagcat gtcgagggtc atgagatcga ctaggccgtc
      661 gttgttgaag tcggcggcgt cgttgcccat ggagtagagg gaggtatggt ccatgcagtt
      721 tgcgagcgat tcggtgaagg tgtgcgatgg gccattattc aggaagagat agtcgggttc
      781 gttgaagtcg ttggagacat agatatccgg ccagccgtca ttgttgaggt cggagatcgc
      841 caggccgagg ccgaaggtga ggacgtcgga gtggatgccg gcctgggccg agacgtcggt
      901 atagacgggg tggtggaggg agtcgttcca gtcgttgcga agcagtttgc attcgaaggc
      961 ggggcggctt tcttttcgcc agccggggtt ttcgatggcg ccggtggtgt attggtggag
     1021 ggagtggttg atgaggaagc agtcgaggtc gccatccttg tcgtagtcga agaaggcggc
     1081 ctgggtggag aagccttcgt cggcgaggcc gtattcggcg gctttttcgg agaaggtggg
     1141 ggcggcgtgg gggtctgcgc cggaggcggg gccgttgttg atgaagagaa ggtttctgcg
     1201 ccgggcgggg ttggcgtcgg cggagcggca gatgtagata tccattttcc catcgccgtt
     1261 gatatcgacc atagtggcgc cggtggccca gccttccacg gaaggtgcgg cgaggccgct
     1321 tttttcggtg atgtcttcga atgtgaaatt gcctttattc agaaagaggc ggttgtggac
     1381 catgttgccg gtgaagagga tatcgggaag gccgtcgttg ttgatatcgc cgatggcgac
     1441 accgccgccg ttgtagaaat agatgtagtt gaggacgttg agggcttcgc cgtcgaagag
     1501 ggtgttctgg aagtcgatgc cggtggcgtc tttgtcgagg cgggtgaaga ggcggttgtc
     1561 ctgcccgggt ttgcagcctg ctgcgagcag gaggaggagt atgatgcgga cgcggtggag
     1621 tatgacgcgg aggaggagta tgatgcgggg gcggtggagc atggtgtcga aataaaagct
     1681 cccatccttt ttggggatgg gagcttcaag ttaggcaatt ttagttctta tcccaccaga
     1741 gcggggtgag ttcgttgtcg gtgcccccca gtgccgtcac gcctgtctgg acgccggcgg
     1801 cgttgttctg gcgttcggtc agcaggaagg ggatccggcg cagccatagt ttgctgggat
     1861 ccgggatcag ctggttgtcc gaattggcgg cggggtagag tttcatgacg tggctgcggc
     1921 gccagtctgc ccaggcctcc atgccgtcgg gaaagagggc cagccatttt tgggtcatga
     1981 tctgttgcaa ttcgacagaa ggatcggcgc tccacgcgat gggtacagtg gatacggcgg
     2041 gtgagttgag gtagtcgcca ggagcaatgg gagtgttggc gctggctata taggtattga
     2101 cagtggtagc gtctgtgatg ccccattggg ccatggagtt cttgatacct gtttcataca
     2161 ggctttgagc cgtaccgccc atattccaac ccaggagggc gccttccgcg cgaaggaaat
     2221 aggcttcggc ggtttcgatg acatgttggg gggtgttggt gtaggaggct tctccgacca
     2281 ccgttccgcc gaccgttaca ctggtagagg accagcgttg tccctggtgg gagttggcgg
     2341 cggccaggtt tggcgacagg gtcaactggg cgacgctgag gccgttgcgg agtccctggt
     2401 agttggcaaa cttcccgaag gtggggtctc cgctgttgac ggtgggcagg aagtactggg
     2461 agatgcgggg atcctggtag cctttcagga cggaggccat ggtggcgctc atccggaact
     2521 cgttccagtc cgacatggtg gaaagaccgt tgatgtcacc gccgacggag gagcgtttga
     2581 tcaggccgtc gtcgtcgggg ctggtggtca tcacgccatc ggccaccgag gcttcggctt
     2641 ctgtctttgc ggtagccggg tcagctttgg agacccgcag ggccaggcga agccggaggg
     2701 tgttggcgaa cttgcgccat ttatcgacgg agccgccata gatcaggtca taggacccat
     2761 aggcattgcc gcccgcgttt gccttcagga tggcgatggc tccatcgagg cgatggaaga
     2821 agtcggcata gatcttatcc tgggcgtcgt aggggaccga tgtggctacg gtgcctgcct
     2881 ggaaataggg gatgggtccc caataatcgg tagccttcat gaaggtgagc acccagacca
     2941 cttcggccat ggcgtgttcg ggcttggcgg aatccgtgtt ggcgaagatg gtctgcagct
     3001 ggggtaatac atccgtatag taggggttga aattggcccc tacccaggcc tggttgatca
     3061 ccaggcgatc ggtgccgaag taggtggctt cgcaggcgaa gtactgtgca tactgatcgg
     3121 cgaagaggtt
//
LOCUS       DATGFG010000405         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_379217, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000405 DATGFG010000000
VERSION     DATGFG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379217"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VKQ52_07585"
     CDS             <1..577
                     /locus_tag="VKQ52_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87083.1"
                     /translation="TLWYPKAGTYTLTLTAKNKDGVKSTVSKKVKVLDRVMKQVLVTG
                     LTPFFWPVGHSLDHANIWVVIRLGANDVHYPIPSGANISFDAPIIYQSAVINGVDSTR
                     LPYAFDLPGKMIVNFPAMAVRSEIGLGYTGVGYGLELYAQDAGGTYLLSSSYAAMYIG
                     QSGSITWPVADYTRDIFIMRYANVSIVCDYE"
     gene            complement(596..1192)
                     /locus_tag="VKQ52_07590"
     CDS             complement(596..1192)
                     /locus_tag="VKQ52_07590"
                     /inference="COORDINATES: protein motif:HMM:NF005754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HLZ87084.1"
                     /translation="MKIILIGASGTIGKKIATAVEKDHELIRVGSKSGDLRVDISSPA
                     SIEKLFKETGPFDTLISAAGDGHFGPLKSMTDADFRKGLDSKLMGQINLVLMGQHHIR
                     PKGSFTLTAGILSQDPVPYGANLSAVNGALEGFGRAAAIELENGVRINVVSPGIVEDS
                     PGYFPYFPGHIPVEMDKVVAAYIKSAFGARTGQVYSVS"
     gene            1354..1701
                     /locus_tag="VKQ52_07595"
     CDS             1354..1701
                     /locus_tag="VKQ52_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ87085.1"
                     /translation="MEKRYHSPTECPITATIDVIGGKWKPVIIYILMDGPLRFGEIHR
                     TIPGMALKVLSRQLKELEEDGIITRTPYPEVPPRVEYALSSKGETLRPVMQLMSAWST
                     EHLLQVSLPAPTV"
     gene            1796..1948
                     /locus_tag="VKQ52_07600"
     CDS             1796..1948
                     /locus_tag="VKQ52_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87086.1"
                     /translation="MTEEQIAYQIQVLKEVGQEIRRSKKASRQFLIDAGIIEDKKKKK
                     QAQKKK"
BASE COUNT          503 a          624 c          443 g          438 t
ORIGIN      
        1 caccctttgg tatccaaaag caggaacgta taccctgacc ctcaccgcaa agaataagga
       61 cggtgtcaaa agcacggttt caaaaaaggt gaaggtgctc gatcgggtca tgaaacaagt
      121 cctcgtcacc ggtctgacac ccttcttttg gcctgtaggc cacagcctcg atcatgccaa
      181 tatatgggtg gtgatccggc tgggggcgaa tgacgtacat tacccgatcc cttccggcgc
      241 caatatcagc ttcgacgccc ccatcatcta ccaaagcgcc gtcatcaacg gcgtcgactc
      301 caccaggctc ccgtatgcgt tcgatcttcc cggaaagatg atcgtcaatt tccccgccat
      361 ggccgtccgg tctgaaattg ggctgggcta caccggcgta ggctatggac tggaattgta
      421 cgcgcaggat gccggcggaa cctatctgct ttcgtcttcc tacgccgcca tgtacatcgg
      481 ccaaagcggc tctatcacgt ggccggtggc agattacacg cgggacatat ttattatgcg
      541 atacgccaac gtaagcatcg tctgcgacta cgaataggct acaccgctgc catccttagc
      601 tcacggaata cacctgtcct gtcctcgccc caaatgcact cttgatatac gcggcgacaa
      661 ccttgtccat ttccaccggg atatgtcccg ggaaatacgg gaaatatccc ggcgaatctt
      721 ctactatacc cgggctgacc acgttaatgc gcaccccgtt ctccaattct atcgctgccg
      781 cccggccaaa accctcgagt gcgccgttga cggcgctcag gttcgcgcca taagggacag
      841 gatcctggga aaggataccc gccgtcagcg taaagctgcc cttgggccgg atatgatgct
      901 gacccatcag cacgaggttg atctgtccca tcagcttgct atccaaaccc tttcggaaat
      961 ccgcatccgt catcgatttg agcgggccaa aatgcccgtc cccggccgcg ctgatgagcg
     1021 tatcgaacgg tcccgtttct ttaaaaagtt tctcgatgga cgccggcgac gagatatcca
     1081 cccggagatc cccgctcttg cttccgaccc ggataagctc atgatccttt tctactgctg
     1141 ttgcaatctt cttaccgatg gtgcccgagg caccgatgag aatgatcttc atatctgatg
     1201 ttgtttaaat ttatttcctt tcatgggcca aagctcgcgc attactggaa aaaagtcaag
     1261 tagttaccca aaagtatagt aatgacaaaa atgtcgtaat tttgctgggt gcgggaaaca
     1321 tttcccccgc ttctcaaaaa aaaattccgc cgcatggaaa aaagatatca ctcgcccacc
     1381 gaatgcccca tcactgccac catcgacgtc atcggcggca aatggaaacc cgtcatcatc
     1441 tacattctca tggatggccc cctccgcttc ggcgagatcc atcgcaccat ccccggcatg
     1501 gcccttaaag tcctctcccg ccaactcaag gaactcgagg aggacggcat catcacccgc
     1561 accccctatc ccgaagttcc cccccgcgtc gaatacgccc tctcctccaa aggcgaaacc
     1621 ctccgccccg tcatgcaact catgtccgcc tggagcaccg aacacctgct ccaagtctcc
     1681 ctacctgctc caacagttta gggcataatt gaactagcac ccaaccgctt tgaggtttca
     1741 atctgcaacc tcaaaaactc cctatctttg taataccaat ccaaaacctt attatatgac
     1801 cgaggaacaa atagcatatc aaattcaggt cctgaaagaa gtcggccagg aaattcgccg
     1861 atccaagaaa gcttcccgcc aattcctcat cgacgcggga atcatcgaag acaagaagaa
     1921 aaagaagcaa gcccaaaaga agaagtagtg tcctaccatc agcctttcca gatcaccggt
     1981 ttccacagtt gcgaccatgc cgaagaag
//
LOCUS       DATGFG010000406         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1531392, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000406 DATGFG010000000
VERSION     DATGFG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531392"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VKQ52_07605"
     CDS             <1..226
                     /locus_tag="VKQ52_07605"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87087.1"
                     /translation="KQQRLFQDLEQKIATLVRRKDALEASLADPGTYSDKNKFVTAET
                     EYKKTSEELEQANRQYEQVFDKIVELEKAL"
     gene            237..2480
                     /locus_tag="VKQ52_07610"
     CDS             237..2480
                     /locus_tag="VKQ52_07610"
                     /inference="COORDINATES: protein motif:HMM:NF039873.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLZ87088.1"
                     /translation="MRHQPLFALLLLLLPFGGTLAQTARLGPRPKIGLVLSGGGAKGL
                     AHIGILKAIDSAGLKIDYITGTSMGSIIGGLYAVGYSADTIENISRRINWDLLLSNQS
                     SMRSMIMDEKDEYARYDVELPWVNHGFQLATGVIEGQELWLKFSELFRPVYKIKDFSR
                     FSIPFKCIATDVSTGEAVVLDTGEIVSALRSSMAIPSVFTAVTYGGRKLVDGGVVRNF
                     PVTDVRKMGADIVIGSNLAGSLRTPDQVTNALQVLLQVAFFREAADTRTQIPLCNIYV
                     HQPLDKFSMGNFNDAREIIDSGLAEGRRLYPRLKALADSLNAIYGPQDVVKNRLPNVY
                     PVKISSYEVKGLTRTTEGFFIQSMDLLTDHYYTAENLSRMVRRAFGTRYYNRITYALD
                     WLPDSTAHITFEAVENPLTFAKLGLNYNQFSGISAILNLTSRNFFTPTSRSLVTMNIG
                     QNFRIRAEHLQYFSRRANFTFTLGPQFDQFKITTYNTKYKEAGLYNQNYFRGDGHFGY
                     STNRDLTIGLGSRWEYTDYDPSITSSLDFKGNNSFLTSYFFIRSNTLDRPSYPRRGMK
                     FEAEADHVFEQTPRVQYFASIVTRSDTSFSSAPYQRFLVNYEQYLPVAQRYTLQMHLQ
                     GGLNLRYHNNIMNEFSIGGLTEQFHNQVTFAGLREGTFYSASIAEGALALRYQLFSNV
                     LVTAKANVLFSNFLDKSNFFTTPDFLSGYALTFTYQFALGPLELSAMYSDQSKAVIGY
                     VNIGIPF"
BASE COUNT          586 a          790 c          689 g          558 t
ORIGIN      
        1 gaagcaacag cggctgttcc aggacctgga acagaagatc gcgacgctcg tccggcgaaa
       61 agatgcattg gaagcatccc tggccgaccc aggtacgtat tcagataaaa acaaatttgt
      121 cacggcggaa acggaatata agaagacaag cgaagaactg gagcaggcca atcgccagta
      181 cgaacaggta ttcgacaaga tcgtggagtt ggaaaaggcc ctttgaatca tccttcatga
      241 gacatcagcc cctttttgcg cttcttctgc tgctactgcc ctttggcggg acgctggcgc
      301 aaaccgcgcg actggggcca agaccaaaga tcgggctggt gcttagcggc gggggagcaa
      361 aggggcttgc ccatatcggc atcctgaaag ccatcgattc ggcagggctg aagatcgact
      421 atatcaccgg cacgagcatg gggagcatca tcggcggcct ctacgctgtt ggctattccg
      481 ccgacaccat cgaaaatatc tcccggcgca tcaactggga cctgctattg tccaaccaat
      541 cctccatgcg aagcatgatc atggatgaga aggatgagta tgcccggtac gatgtcgaac
      601 taccctgggt gaatcacggg ttccagctag ccaccggcgt catcgaaggc caggaacttt
      661 ggctgaaatt ctccgagctg ttccggcccg tatacaagat caaggacttc tcccggtttt
      721 ccatcccctt taaatgtatt gccacggatg tgtcgaccgg cgaggccgtc gtcctggaca
      781 cgggagagat cgtctccgcg ctgcgctcga gcatggccat tccctccgtc ttcaccgccg
      841 tgacctatgg cgggcggaag ctggtggatg gcggtgtggt ccgcaatttc ccggtgaccg
      901 atgtccgcaa aatgggggcc gatatcgtca tcggcagcaa cctcgccggc tcgctgcgga
      961 cacccgacca ggtgaccaat gccctgcagg tgttgctgca ggtagcattc ttccgggaag
     1021 cggcggacac gaggacgcag atccccttgt gcaatatcta tgtccaccag ccgctcgata
     1081 agttcagcat gggcaatttt aacgatgccc gggagatcat cgattccggc ctggcggaag
     1141 gacgccgtct ctatccccgg ctaaaggcgc tggcggattc gctgaacgcc atctacggcc
     1201 ctcaggacgt ggtaaagaac cggcttccca acgtctatcc cgtcaagatc tcgtcctacg
     1261 aggtaaaggg attgacacgc accaccgaag gcttctttat ccagagcatg gacctcctga
     1321 ccgaccacta ctacaccgcg gagaacctct cccgcatggt gagaagggcg ttcgggacaa
     1381 gatattacaa ccggatcacc tatgcgctgg actggctgcc ggacagcacc gcccatatca
     1441 cgttcgaggc ggtggagaat cccctcacgt ttgcaaaact ggggctcaat tataaccagt
     1501 tcagcggtat cagcgccatc ctcaacctta ccagccgtaa tttctttacg ccaacctccc
     1561 gcagcctcgt gaccatgaac atcggccaga attttcgcat ccgggcggaa cacctgcaat
     1621 atttcagccg gcgggccaat ttcactttca ccctgggtcc gcagttcgat cagttcaaga
     1681 tcaccaccta caataccaaa tacaaagagg cggggctgta taaccaaaac tatttccggg
     1741 gggatggcca ttttggctat tccaccaacc gcgacctgac gatcggcctg ggcagccggt
     1801 gggagtatac cgactacgat ccttccatca cttccagcct cgatttcaaa ggcaacaaca
     1861 gtttcctgac cagctatttc tttatccgga gcaataccct cgaccggccc agctatcccc
     1921 gccgcgggat gaaattcgag gccgaggccg atcatgtctt cgaacaaacc ccaagggtcc
     1981 agtattttgc ttccatcgtt acccgcagcg ataccagctt ttcctcggcg ccctaccagc
     2041 ggtttctcgt caactacgag cagtacctgc cggtggccca acgctacacc ctgcaaatgc
     2101 atctccaggg cggtctcaat ctccgttatc acaacaatat catgaatgag ttctccatcg
     2161 ggggattgac ggagcaattt cacaaccagg taaccttcgc cggcctgcgg gaaggaacat
     2221 tttactcggc cagtatcgcg gaaggagcgc tggcgttgcg ctaccagttg ttcagcaacg
     2281 tgcttgtaac ggctaaggcc aatgtgctct tcagcaattt cctggacaag agcaatttct
     2341 ttacgacacc cgacttcctc tcgggctatg cccttacgtt tacctaccag ttcgcgcttg
     2401 gtccgctgga gctcagcgcc atgtactccg accagtcgaa agcggtcatc gggtatgtca
     2461 atatcgggat accgttttga tcgcggcggg ctacctcgcg gcaggatccc tcttcgatcg
     2521 ggccggcaga atccctctct gatcgggccg gctatttgcc gatcttctgg ctcacgacct
     2581 gcaggatcgc cgcttcggcc gcgatggttc tctcctcgat ggc
//
LOCUS       DATGFG010000407         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_512622, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000407 DATGFG010000000
VERSION     DATGFG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512622"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VKQ52_07615"
     CDS             complement(<1..994)
                     /locus_tag="VKQ52_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLZ87089.1"
                     /translation="MLLFNRYQYEPSTDMIGKGEFTRVYEALDTYIYLPVAVKVYRAI
                     DSAEKFGFVHVEKIMGMDHPNICRYLHIGEIRKESSAGENEKMQVCVMELLQDGDFAS
                     YYQAHRSPELLRKMILDILHGLTYLHAQRIVHRRLKPSNMLVGVTTQGPVVKITDFGL
                     GSGKATLRDARFSSMVVEVTDMAPEQFNPKEYGFNGNVTYHVDFWALGLAVYAALSDN
                     DVLFKNSPGDSREQVIRNILSPRLPEKVLRLPAPFNRFVMRCLAKRAEDRPQNTRELI
                     ELLAEPITIVEPGYDEVVRGEPREDTGGDEIVTRSSGVAVSAPAQMTVAEIPQVE"
     gene            complement(1000..1746)
                     /locus_tag="VKQ52_07620"
     CDS             complement(1000..1746)
                     /locus_tag="VKQ52_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HLZ87090.1"
                     /translation="MQISKIDYLYEIGARKNMEDYLWPLPGTASGQDRIFIVCDGVGG
                     AENGEVASRLVAEWVGRSLLKTPPAEIGLPLINELLEAARMKLVEFALLRGYGTDMAT
                     TLALLVLRKDGAFIAWCGDSRVYHLRKDEILFRTDDHSLVHSLIRQGELTEVEAREHP
                     QRNLLLKAIRADDLRPEAEGHWIDDIREGDYFLLCTDGLLENIPDDDLLSLLQQNERG
                     EIDLAKALRRYCYDKTRDNYSMYLIQVSIK"
     gene            complement(1749..>2423)
                     /locus_tag="VKQ52_07625"
     CDS             complement(1749..>2423)
                     /locus_tag="VKQ52_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ87091.1"
                     /translation="YAKAAAAYEQALRLSAGGKQLADDVLVAEMYNSLGHCYCKQGAY
                     EKAVKNGKEAIGHNRNFAEAYCNRGYAYYKWGKLPEAIADLQKAIALDSKPVYWHYVL
                     GMALLAKDDRGGAGAQFALCAQKDNVPASYSMEMGIICLNMGKYDSAYSYCRKAFESD
                     STNGYAAYYTAVALSVQGKAEESMPWFEQAFRRKIPGHSELKKERLLANIRRNGKFRE
                     LLNKYY"
BASE COUNT          531 a          802 c          556 g          534 t
ORIGIN      
        1 cctccacctg tggaatttcc gccactgtca tctgcgcggg cgcactcacc gccacaccgc
       61 tgcttcgggt tacgatctca tcgccaccgg tatcctcccg gggctccccc ctgaccacct
      121 catcataccc cggctccaca atcgttatgg gctcggccag caactcgatc aattcccgtg
      181 tgttctgcgg ccggtcctcc gcccgcttcg ccaggcagcg catgacaaac cggttaaagg
      241 gcgccggcaa ccgcaatacc ttctccggca gccgcggcga gaggatattc cggatcacct
      301 gttcccggct gtccccggga ctattcttaa aaagcacatc gttatcgctg agcgccgcat
      361 acacggccag gccaagcgcc cagaaatcga catgataggt gacattccca ttgaagccat
      421 attcctttgg attgaattgc tcgggcgcca tatccgtcac ctcgaccacc atcgacgaaa
      481 aacgcgcatc ccgcaaagtg gccttccccg atccgagtcc aaaatccgtg atcttcacca
      541 ccggcccctg ggtcgtcacc ccgacaagca tattggacgg cttcagccgc cggtgaacga
      601 tccgttgcgc atgcaggtag gtcagcccat gaaggatatc gagtatcatc ttgcgcaaca
      661 gctccgggct ccggtgagcc tggtaataag aggcaaaatc cccatcctgc aaaagctcca
      721 tcacacacac ctgcatcttt tcgttctcgc cggccgaact ctctttcctg atctccccga
      781 tgtgcaggta acggcagata ttggggtgat ccatgcccat gatcttttcc acatgtacaa
      841 agccaaattt ttccgcggaa tcgatcgccc gatagacctt taccgccacc ggcagataga
      901 tatacgtatc aagcgcctca tacacccgcg taaactcccc cttgccgatc atatcggtgg
      961 aaggctcgta ctggtaccga ttgaatagta acatggtctt tattttatgc ttacctggat
     1021 taaatacatg gaataattgt ccctcgtctt gtcataacaa tatcgccgga gggccttcgc
     1081 cagatcgatc tccccacgct cattctgctg cagcagggac aacaggtcat catccgggat
     1141 attctcgagc agcccatccg tacaaaggag gaaataatcg ccttcccgga tatcgtcgat
     1201 ccagtggccc tcggcctcgg gccgcagatc atcggcacgg atggccttta aaagcaggtt
     1261 ccgttgcgga tgctcccgcg cctccacctc cgtcagctca ccctgccgga taagcgaatg
     1321 cacaagcgaa tgatcgtccg tccggaaaag gatctcatcc ttccgcaggt gatagacccg
     1381 gctatccccg caccatgcga taaaggctcc gtcctttctc aacaccagca gtgcaagcgt
     1441 cgtagccata tccgtaccgt atccccgcag caaggcaaac tccaccagct tcatccgcgc
     1501 cgcctccagc aattcgttga tcaatggcaa cccgatctcc gctggcggcg tcttcaacag
     1561 cgagcggccc acccactccg ctacaagccg gctggccacc tccccattct ctgctccgcc
     1621 caccccgtca caaacgataa agatccggtc ctggccggac gcagtgccgg gtaagggcca
     1681 gagataatcc tccatgttct tcctggcgcc gatctcatat agataatcga ttttgctgat
     1741 ctgcatgatc aatagtattt atttagaagc tccctgaatt tgccattacg ccggatattc
     1801 gccagcagcc gctccttctt caattcgcta tgcccgggta tctttctccg gaaggcctgc
     1861 tcgaaccagg gcatcgactc ctccgcctta ccctgcacgc tgagggcgac ggctgtataa
     1921 taagcggcat aaccgttcgt actgtcactt tcaaaagcct tccggcaata ggaataagcc
     1981 gaatcatatt tccccatatt cagacagatg attcccattt ccatggaata ggaggccgga
     2041 acattatcct tttgcgcaca aagggcgaac tgcgccccgg ccccgccccg gtcgtccttc
     2101 gcaagcagcg ccatacccag cacataatgc cagtaaacgg gcttgctgtc gagggcgatc
     2161 gccttttgca gatcggcaat agcctccggc agcttgcccc atttatagta agcgtatccc
     2221 cggttgcagt aggcctcggc aaaattgcgg ttatggccga tcgcctcctt gccatttttc
     2281 accgcctttt cataagcgcc ttgcttgcaa taacaatgcc cgagggaatt atacatttcc
     2341 gccaccagaa cgtcatccgc cagctgcttt ccgccggcgg aaagccgaag ggcctgctca
     2401 taggccgccg ccgcctttgc ata
//
LOCUS       DATGFG010000408         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_78791, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000408 DATGFG010000000
VERSION     DATGFG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78791"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VKQ52_07630"
     CDS             <1..364
                     /locus_tag="VKQ52_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87092.1"
                     /translation="FSFELRNRIRAEDAVSWDGVGILKKDGAGNVLFEPVASPLLFMQ
                     PTPAVRVNRQDAQHTLLVGDRERTSGEMNELLHDEEPGRRRMAWWVIALIIAGIGLAV
                     LGWYFYSHGLSTGNQNKF"
     gene            393..1283
                     /locus_tag="VKQ52_07635"
     CDS             393..1283
                     /locus_tag="VKQ52_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLZ87093.1"
                     /translation="MPLIHYTTCPVCHSTELEPALTATDHTVSHESFAIWQCRQCTLR
                     FTQAVPDAASIGEYYRSEEYISHSNTNKGLVNRLYHLVRRQTLSDKFRLIASATRKRQ
                     GRLLDIGAGTGAFVAYMQEKGWEVTGLEPDETARERARTDHRVQLLDTDHLFSLPPDS
                     FDAVTLWHVMEHVHSLHPYVEQLKTLVRRGGRIFIAVPNYTSYDARVYREAWAAYDVP
                     RHLYHFSPDAMETLLEQHGLQLQYSEPMWYDAIYISMLSEKYRGRGNTLRGVFNGFSS
                     NARAFIDKSKCSSLIYVVSK"
     gene            complement(1287..2117)
                     /locus_tag="VKQ52_07640"
     CDS             complement(1287..2117)
                     /locus_tag="VKQ52_07640"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HLZ87094.1"
                     /translation="MNKLSLFFVAGCFLVACNNPSKDDTPATNPDKPADNTPPTLDYT
                     VIKAYPHDTSAYTEGFLFHDGKLYESTGTEPDMPADRRSLFGTVDLATGKITPKVELD
                     RNKYFGEGIVFLNGKVYQLTYTTKIGFVYDATTYKKIGEFTFPSKEGWGMTTDGTYLI
                     MSDGTSNLSYLDPNTFRLVKVLGVTDNNGPVSNINELELIKGYLYANQWQTNYILKID
                     PASGKVVARLNMDSLVSQVRNQYPGAEVLNGIAYDSTTGKVYVTGKLWPGIYEIKFPL
                     "
BASE COUNT          475 a          566 c          684 g          623 t
ORIGIN      
        1 gttctctttc gaacttcgta accgcatcag ggccgaggat gccgtatcct gggatggggt
       61 tggcatcctg aagaaggatg gtgcgggtaa tgtccttttc gagccggtag cctcaccgct
      121 tctttttatg cagcctactc ctgccgtgcg ggtcaatcgc caggatgccc agcataccct
      181 gctggtcggg gaccgggagc gcaccagcgg ggagatgaac gagctgctgc atgatgaaga
      241 accgggtcgc aggaggatgg cctggtgggt cattgcgctg atcattgccg gcatcggtct
      301 tgccgttctt ggctggtatt tctattccca tggtttgtca accggtaacc aaaacaaatt
      361 ctaatcccgg cctgccgaaa tcgatctcct ttatgccctt gatccattat accacctgcc
      421 ccgtctgtca ttcgaccgag ctggagccgg cgcttaccgc gacagatcac accgtttccc
      481 acgagtcttt tgccatctgg caatgccggc aatgcacact gcggtttacc caggctgtac
      541 cggatgcagc atcgataggg gagtattacc gcagcgaaga gtatatttcc catagcaata
      601 ccaataaagg gcttgtcaac cggttgtatc acctggtgcg taggcagacc ctgtcggaca
      661 aattccggct gatcgcttcg gctacccgga agcggcaggg gaggttattg gatattgggg
      721 ccgggacggg ggcttttgtg gcctacatgc aggagaaggg gtgggaggtg acggggctgg
      781 agcccgatga gacggcgagg gagcgggcgc ggaccgatca ccgggtccag ttactggata
      841 ccgaccattt attctccctg ccgcctgata gttttgacgc cgtcacgctt tggcatgtga
      901 tggagcatgt gcattccctg catccctatg tcgagcagct caagacgctg gtacgccgtg
      961 gcggccggat ctttattgcc gtccccaact atacttcata tgatgcgcgg gtataccggg
     1021 aggcctgggc ggcttatgat gtgccgcggc atctctatca tttctccccg gatgcgatgg
     1081 agacattgct ggagcagcat gggcttcagt tgcagtatag tgagccgatg tggtatgacg
     1141 ccatctatat cagcatgctg agtgaaaaat accgggggcg gggcaatacc ctgaggggtg
     1201 ttttcaatgg gttttcctcc aatgcccggg cctttatcga caagtcgaaa tgcagcagcc
     1261 tgatctatgt tgtctcgaag tagcggctag agagggaatt ttatctcata gatgcccggc
     1321 catagttttc ccgtgacata tactttcccc gtggtggagt catacgcgat gccattcagg
     1381 acctcggcgc cgggatattg attccttacc tggcttacga gggagtccat gttgagtctt
     1441 gccacgactt ttccgctggc gggatctatt ttgaggatat agtttgtctg ccactggttg
     1501 gcgtagagat agccttttat cagttccagt tcgtttatat tgctgacggg gccgttgtta
     1561 tcggtaactc caaggacctt gacgaggcgg aaggtattgg ggtcgagata actgagattg
     1621 ctggtgccgt cgctcatgat gaggtaggtt ccgtctgtcg tcatacccca gccttccttg
     1681 ctggggaaag tgaattcccc gattttctta taggtggttg cgtcgtatac gaagcctatt
     1741 ttggtggtgt aggtgagctg gtagaccttt ccgttgagga agacgatgcc ttcgccgaag
     1801 tacttattgc gatcgagttc cacttttggg gtgatcttac ccgtagccag gtctacggtt
     1861 ccgaagaggg agcggcgatc ggcgggcata tcgggttcgg tgcccgtgct ttcatatagt
     1921 tttccatcat ggaacaggaa gccttcggta taggcgctgg tgtcgtgcgg gtaggctttt
     1981 atgacggtat aatccagggt gggcggagta ttgtcggcgg gtttatccgg gtttgttgcc
     2041 ggggtatcgt cttttgaggg attattacag gctacgagga agcatccggc gacaaaaaaa
     2101 aggctcagtt tgttcataca atgcgcaata ggggacaaaa ataagggtta aaaaacttgc
     2161 attattcaaa aaaaatgtat tgttttacag tgtattatgc gttgatcagg gccctctgca
     2221 ccacacacca caattctctc ttccacaatg ggttctgttc ctcggtctct cttctgctga
     2281 aatatccttc ctgaaagcta atccattatt cctgtcagtt gccgctgaat gggcgggggt
     2341 ttagttgc
//
LOCUS       DATGFG010000409         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_935315, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000409 DATGFG010000000
VERSION     DATGFG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935315"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VKQ52_07645"
     CDS             <1..1185
                     /locus_tag="VKQ52_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87095.1"
                     /translation="RTYKLLLLVFIVLSPCLLEIYSMIWSETVYLLLSVLFIIAAHGY
                     FQTHSMRQLLGMALVAALASVTRYAGVSLVALGGLLLLCDGRLRWGARKLGHIALYGV
                     VAVSFLALNLYRNLRLTQTLTGYREPGLTPFGDNLHGFGSVLADWLPFFNEHYQAAPF
                     LAVLFILLITGIFLYRLVRRRDFFSYNNLSLSYFVVYTTFILVTATVSRFQALDSRLL
                     SPLFLPWLWGSTQWIPAALNRCPSRWKKPAIAISLIAAGCFIYGEIRTFRDNWEGIHY
                     AGIPGYTENNWRQSETMAWLREHRDSLEKAGPIYSDAFEGLWFLADLRSDLIAHKDNP
                     EDIKYMLRENHFSVVWFDDAVNDDLISIDYMRSRKQLVRELHFKDGAVYFFRTPPGSP
                     PH"
     gene            1225..1977
                     /locus_tag="VKQ52_07650"
     CDS             1225..1977
                     /locus_tag="VKQ52_07650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HLZ87096.1"
                     /translation="MFLIALAVAPGIAVCLFIYSLNKYGRESMRYLVMAFILGMAATV
                     PALLVQLRVEDVRTEPWRHSIAAYIWYAFAVVALSEEGSKFLVLRLYAYPKRVFREPF
                     DGVVYGVMIGMGFATVENIEYVHKFGLETGVSRFFLAIPAHAAFAVLMGYPVGRAKFE
                     PGRSFFLLAKGLLVAVLFHGSFDFFLFIQQNREATKYLSTGMLSFGAFATFYIAVRLA
                     MRALRMHQAKENQPNNYKQSENDGGNVIDKTG"
     gene            1955..>2192
                     /locus_tag="VKQ52_07655"
     CDS             1955..>2192
                     /locus_tag="VKQ52_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87097.1"
                     /translation="MLSIRPADLDDINTIGFLAQQVWPDTYGQILNPDQLQYMMGLFY
                     SPGALRRQMLDERQQFLLVEQGEEAIGFAAWGPTD"
BASE COUNT          386 a          604 c          624 g          578 t
ORIGIN      
        1 aggacctaca aactcctgct cctcgttttt atcgtgctca gtccctgtct gctggagatc
       61 tattcgatga tctggtcgga gacggtctac ctgttgttat ccgtcctttt tatcatcgct
      121 gctcacggct attttcaaac gcactcgatg cgccagttgc tcggcatggc cctggtggcc
      181 gcccttgcca gtgttacgcg ttatgccggc gtcagcctgg ttgcccttgg cgggctgctg
      241 ctgctttgcg acgggcgcct gcggtggggt gccaggaagc tggggcatat cgccctctat
      301 ggcgtcgttg cggtttcctt tctcgccctg aacctttatc gtaacctgcg gcttacgcag
      361 accctgaccg gctaccggga gccggggcta acgccatttg gcgacaacct tcatggcttt
      421 ggcagtgtcc tcgccgactg gctgcctttt ttcaatgagc actaccaggc ggcccccttc
      481 ctggccgtcc ttttcatctt actgattacg ggcatcttcc tgtaccggtt ggtccggcgc
      541 cgggattttt tttcctacaa caatctttcg ctgagctatt tcgtcgtgta taccaccttt
      601 atcctggtta ctgccacggt ctcccgtttt caggccctgg acagccggct cctgtctccc
      661 ctcttcctgc cctggctctg ggggagtacg caatggatcc cggccgcgct gaaccgctgt
      721 ccttcccgct ggaagaagcc ggcgattgcg atcagcctta tcgccgcagg ctgttttatc
      781 tatggggaga tccggacctt ccgggacaac tgggaaggca ttcactatgc cgggataccc
      841 ggttatacgg agaacaactg gcgacagtcc gagacgatgg cctggctgcg ggagcaccgg
      901 gattccctgg aaaaggcggg gccgatctat tcggatgcct tcgagggatt gtggttcctg
      961 gcggatctcc ggtccgacct catcgcccac aaggacaatc ccgaagacat caaatatatg
     1021 ttgcgtgaga accatttttc ggtggtatgg ttcgacgatg ccgtcaatga cgacctcatc
     1081 agcatcgact atatgcggag ccggaagcaa ctggtgcggg aattgcattt caaggatggg
     1141 gcggtttatt ttttccgtac tccacccgga tcgccgcccc attgagttct cgggtagtat
     1201 cgatatttca gctattttta aggaatgttt ttaattgcat tggctgttgc gccgggtatt
     1261 gcggtatgtc tttttatcta ctccctcaat aaatacggga gggagtcgat gcgctacctg
     1321 gtgatggcct ttatcctcgg gatggcggcc accgtgccgg ctttgctggt gcaattgagg
     1381 gtggaggatg tccgcacgga accctggcgt cattccattg ccgcttacat ctggtatgct
     1441 tttgccgtcg tggcccttag cgaggagggc agcaaattcc tggtgctgcg gctctatgct
     1501 tatcccaaac gggtcttccg cgagcccttc gacggcgtgg tctatggggt gatgatcggg
     1561 atgggttttg ccaccgtgga gaacatcgaa tatgtccata agttcgggct ggagacgggg
     1621 gtgtcccggt tcttcctggc cattcccgcg catgccgctt ttgccgttct gatgggttat
     1681 cccgtcggcc gcgccaagtt cgaaccgggc aggtcgttct ttttactggc aaagggattg
     1741 ctggtggccg tattgttcca tggcagtttt gattttttcc tttttatcca gcaaaaccgg
     1801 gaggccacaa aatatttgtc gacgggaatg ctttcctttg gggcgtttgc caccttctat
     1861 atcgcggtgc ggctggccat gcgggccttg cggatgcacc aggcgaaaga gaaccaacca
     1921 aataactata agcaatctga aaatgatgga ggaaatgtta tcgataagac cggctgacct
     1981 ggatgatatc aataccatcg gctttttagc ccagcaggtc tggcccgaca cctatggcca
     2041 gatcctgaac ccggaccagt tgcagtacat gatggggctt ttctatagcc cgggggccct
     2101 tcgccggcag atgctggacg agcggcagca gttcctgctt gtcgaacagg gagaggaagc
     2161 catcggcttt gctgcctggg gcccaaccga cg
//
LOCUS       DATGFG010000410         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1276628, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000410 DATGFG010000000
VERSION     DATGFG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276628"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..443
                     /locus_tag="VKQ52_07660"
     CDS             120..443
                     /locus_tag="VKQ52_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87098.1"
                     /translation="MEKRSFEDIEKELLTHLSAANLPKDHLAGISRSIAASYNAGLQI
                     VDWWVYGIPAFEKLVIQAQLPIIETKALQGLVQNEHFKAIEIHRKGIPRPDFFQLQLT
                     VERTT"
     gene            616..1776
                     /locus_tag="VKQ52_07665"
     CDS             616..1776
                     /locus_tag="VKQ52_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLZ87099.1"
                     /translation="MPLAQAASTDLCEALPPGRVSRIIQIHPSLRCNLSCKHCYSSSG
                     PGAKPGLEPAPLINLLEQAAPLGYNVVSLSGGEPFLYPHLGPLAQAAKQLGYYNSVTT
                     NAMLLSAASKQQILPFFDLVAVSVDGREANHDLLRDHPGSFAKMLHGVDILKAAGREI
                     GFIHTALPRSWQLLPWLAEFAQSHGARLLHIHPLEHTGRAANGLFLPEAADTRFTADT
                     LLTADKHLTTDTHFTDNRFTAENLHKLYIAAHYLQAYCSPDLFIQLDLLHRDNIIGNR
                     SFHFPHPKRRPRPTATTFSHLFGELIVNEHGDLLPIAHGCSNYFRIGNIHSGRPLDEM
                     IEAFIDEKFTHLLELHNTTYDAIAADPSLEIVNWSERLLENSHVISSPHPSA"
     gene            complement(1748..>2421)
                     /locus_tag="VKQ52_07670"
     CDS             complement(1748..>2421)
                     /locus_tag="VKQ52_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87100.1"
                     /translation="HRFLFATDFLAFMLQGKETVGTLLSFQLGFSGSLNFAPGSPYAL
                     PAGDGGALGLALHPYSPTLYVGFPVAGSVGAYSIDPFTGALSLQTTVKAGAAACWLKT
                     NSRGTRLYVLNSGNNAVQVFNTESPNAPVSMSTLVLKEPGTTYTTASGGTAISEADFA
                     LGFSPDGWTLYVVSQNTNPDLTAPNFNFLHVLRVAGDGSLTEPGEPLKLPVGADVRPM
                     GVATR"
BASE COUNT          567 a          924 c          496 g          434 t
ORIGIN      
        1 aaatccaccc tatcttgcac tcgaaactta cttcttattc ctgttcctac gtcacatctc
       61 tgatcccaca cacacgattg ctgccttccc ggcccgaccc aaaccgacaa tgcattttca
      121 tggaaaaaag aagtttcgaa gacatcgaga aagagctcct tacccattta tcagccgcca
      181 atctcccaaa agaccatctc gccggcatct cccggtccat cgccgcctcc tataacgcgg
      241 ggctgcagat cgtcgattgg tgggtctatg gcattcccgc ctttgaaaaa ctcgtcatcc
      301 aggcccaatt gccgatcatc gaaacaaaag ccctgcaagg cctcgtccag aacgagcact
      361 tcaaggccat cgagatccac cgaaaaggca tcccgcgccc ggacttcttt caactacaat
      421 tgacagtcga acggacaaca taaatgagag tcgaccgaac aacataaaca aaaggacatt
      481 gcaaaaaccc caacccgacc ccgtagtcac tcattgcaaa ccccgatcgc ccctttaaac
      541 aacccctacc caaactccgt agccccaacc caaaaaccgt agccctcatt gtacaaaccc
      601 caagcctctt gtcgaatgcc ccttgcccaa gccgcgtcta ctgatctctg tgaggctctt
      661 cccccgggaa gggtctccag gatcattcag atccacccca gtctccgctg caacctctct
      721 tgcaaacatt gctattcctc gtcgggtccc ggcgcaaaac ccgggctcga accggcgccg
      781 ctgatcaacc tcctcgaaca ggcggctcct ctcggctata acgtcgtcag cctctcaggc
      841 ggcgaaccct tcctctaccc gcatctcggc cccctggcgc aggccgcgaa acaactcggt
      901 tattataatt cggtcaccac caacgccatg ctcctctccg cggcgtccaa acaacagatc
      961 ctccccttct tcgacctcgt cgccgtcagc gtcgacggcc gtgaagctaa ccacgatctg
     1021 cttcgtgatc accccggctc cttcgccaaa atgctccacg gcgtcgacat cctgaaagca
     1081 gccggccgcg agatcggctt catccacacc gccctccccc gaagctggca gctcctcccc
     1141 tggctcgccg aattcgcgca aagccatggg gcccgtctcc tgcatatcca tccgctcgaa
     1201 cacaccggcc gcgccgccaa cggactcttc ctgccggagg ccgccgacac ccgcttcacc
     1261 gccgacaccc tcctcaccgc cgacaaacac ctcaccaccg acacccactt caccgacaac
     1321 cgcttcactg ccgaaaacct tcacaaactc tacatcgccg cacactacct gcaagcgtat
     1381 tgctcaccgg atctctttat ccaactcgac ctcctccatc gtgacaacat catcggtaac
     1441 cggagcttcc atttcccgca cccaaaacgg cggccccgcc ccacggccac aaccttctcg
     1501 catctcttcg gcgaactcat cgtcaacgaa cacggcgacc tccttcctat cgcccatggc
     1561 tgctcgaact acttccgcat cggtaacatc cacagcggcc ggcccctcga cgagatgatc
     1621 gaagccttca tcgacgaaaa atttacccat ctgctcgagc ttcacaacac cacctacgac
     1681 gccatcgccg ccgacccctc cctcgagatc gtcaattgga gcgaacgcct cctcgaaaat
     1741 agtcacgtta tctcgtcgcc acacccatcg gcctgacatc cgcgcccacc ggcaatttca
     1801 acggctcgcc cggctccgtc agcgacccat cgcccgccac ccgcaacaca tgcaggaaat
     1861 tgaaattcgg cgcggtcaga tcgggattcg tattctggct caccacatac aacgtccacc
     1921 cgtccggcga gaacccaagc gcgaaatccg cctcggaaat ggccgtccca cccgaagccg
     1981 tggtataggt ggtccccggc tccttcaaca ccagcgtgct catcgacacc ggcgcattcg
     2041 ggctctcggt attgaacacc tgcaccgcgt tgttccccga attcagcaca tacaaccgcg
     2101 tgccccgcga attcgtcttc agccagcagg ccgccgcccc ggccttgacc gtggtctgca
     2161 aactcagggc gcccgtaaaa ggatcgatgg aatacgcccc caccgaaccc gccaccggga
     2221 aacccacata cagggtcggg ctatacggat gcagggccag tcccaacgca cccccatctc
     2281 ccgccggcaa cgcataggga ctacccggcg caaaattcag gctgcccgaa aaacccagct
     2341 ggaaggaaag cagcgtccca accgtctcct taccctgtag cataaacgcc aggaaatccg
     2401 tcgcaaacag gaaccggtga t
//
LOCUS       DATGFG010000411         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1034064, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000411 DATGFG010000000
VERSION     DATGFG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034064"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /gene="nadC"
                     /locus_tag="VKQ52_07675"
     CDS             complement(<1..796)
                     /gene="nadC"
                     /locus_tag="VKQ52_07675"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HLZ87101.1"
                     /translation="MSNFFDEQLQLLVSNALKEDVGDGDHSTLSCIPPTARGKAVLHI
                     KQDGILAGMEIAEKILKMKEPTARFTAYKQDGDAMRDGEEAFIAEATIHTILQCERLI
                     LNCMQRMSGIATLTRQYVDRLQKYKTRVLDTRKTTPNFRLLEKEAVRIGGGINHRFGL
                     YDMILLKDNHIDYAGSIEAAIEKASAYRQAYKPHLKIEVETRNPNEVRRVLAIGKVDR
                     IMLDNFTPRQLSEAMSLIGDRFETEASGGINLNNIEDYARTGVDYIS"
     gene            complement(865..1254)
                     /locus_tag="VKQ52_07680"
     CDS             complement(865..1254)
                     /locus_tag="VKQ52_07680"
                     /inference="COORDINATES: protein motif:HMM:NF027349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HLZ87102.1"
                     /translation="MKSIFGLAVLTISLVLVSFRPTYTLDEITAAIRVGNAGELSRYL
                     DSRVDISLPEKSDTYSKIQAEMIIRDFFTTHGVQNFLIKLRGPNGGGSEFCVGVLQTR
                     NGDYRTTLFVKQKGDRQLLQELRFQAQ"
     gene            1417..>2372
                     /gene="gpmI"
                     /locus_tag="VKQ52_07685"
     CDS             1417..>2372
                     /gene="gpmI"
                     /locus_tag="VKQ52_07685"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HLZ87103.1"
                     /translation="MPDQKKVILVIMDGWGLGSRASSDAIRNASTPFVSSLYQQYPNT
                     TLVTYGEDVGLPEGQMGNSEVGHLNLGAGRIVNQELQRINVAIRDGSFAKNPVLLEAI
                     RGAKKEGKPLHLLGLVSDGGVHSHTRHLKAIVDTCGKEGLKEVYIHAFTDGRDTDPKS
                     GLGFIRDLQAHLNKTVGKIATVSGRYFAMDRDKRWERVQLAYNALVRGIGEKSTDALA
                     AVEQSYQAGITDEFIKPTVIVASDQQPLALIKDGDYALCFNFRTDRCREITEVLTQQD
                     LPDPGMKKLSLHYTTMTQYDAKFKGVKVIFENDDLKNTLGEI"
BASE COUNT          555 a          670 c          637 g          510 t
ORIGIN      
        1 cacttatata gtccacgccg gtgcgggcat agtcttcgat attgttgaga ttgataccgc
       61 cgctggcttc cgtctcgaac cggtcgccga taagggacat ggcctccgac aactgccggg
      121 gggtgaaatt atcgagcatg atgcggtcca ccttgccgat agccagcacc cttcgcacct
      181 cattgggatt tcttgtctcc acttctatct tcagatgggg cttatacgcc tggcggtagg
      241 cactggcttt ttcgatggcg gcttcgatgc ttccggcata gtcgatatgg ttgtccttca
      301 acaggatcat atcatagagc ccaaagcggt gattgatccc gccgccgatg cggacggcct
      361 ccttttcgag cagccggaaa ttgggtgtgg tcttccgcgt gtccaggacc cgggtcttgt
      421 atttctgcag ccggtcgaca tattgccggg tcagggtggc gatgccgctc atccgctgca
      481 tacagttgag gatgaggcgt tcacactgga ggatggtatg gatggtggct tcggcgataa
      541 aggcctcctc cccgtcccgc atggcatccc cgtcctgttt gtaggcggtg aagcgggccg
      601 tgggttcttt cattttcagg atcttctcgg cgatctccat accggcgagg atcccatcct
      661 gcttgatatg caggacggcc ttgccccggg ccgtcggggg gatacaggat aaggtgctat
      721 ggtcgccgtc gccgacatct tccttcaggg cattgcttac caggagctga agttgctcgt
      781 caaaaaagtt ggacataccg caaaaataca aaggccgggt cacaaagtga cccggccctc
      841 aggattaccc taataggaat ctctttactg tgcctggaac cgcagctcct gcagcagttg
      901 tctgtcccct ttttgcttga caaagagggt agtacgatag tcgccgttac gggtttgcaa
      961 tacccctacg cagaactctg aaccgccgcc gttcggaccc cgaagtttga taaggaagtt
     1021 ctgtacccca tgggtggtga agaagtcccg aatgatcatt tccgcctgta tcttgctata
     1081 ggtatcactc ttttcaggga gggagatatc tacacggcta tccagatacc tggataattc
     1141 gccggcattc ccgactcgaa tagcagcagt tatttcgtca agcgtatacg tggggcggaa
     1201 cgaaaccagg acaagcgaga tcgtaagtac cgccaaccca aatatgctct tcatgttaat
     1261 aaaatattgg tcgtcgtact taatcccaaa aatatgccaa actgccagga aggcaattaa
     1321 aatggcaaaa acagggcaca gcccggtgaa tggcgtattt tcgcggctcg aataacagca
     1381 gttaacagca cttaacagca gtaaaacccg ctttttatgc cagatcagaa gaaagtcatc
     1441 ctcgtcatca tggatggttg gggcctcggc agccgggctt cctccgacgc catccgcaac
     1501 gccagcaccc cctttgtcag ctccttatat cagcaatacc ccaatacgac cctggtcacc
     1561 tacggagaag acgtagggtt gccggagggc cagatgggca attccgaggt cggtcacctg
     1621 aatctcggcg ccggacggat cgtcaaccag gagctgcagc gcatcaatgt agccatccgc
     1681 gacggctctt ttgccaaaaa cccggtcctg ctggaagcga tccgcggggc taaaaaggaa
     1741 ggaaaacccc tgcacctcct cggcctcgtc agcgacggcg gggtgcacag ccatacccgg
     1801 cacctaaaag ccatcgtcga cacctgcggg aaggaaggac taaaagaagt atatatccat
     1861 gcctttacgg acggccggga caccgacccg aagagcggac tcggttttat ccgggacctg
     1921 caggcccatc tgaacaagac cgtcggcaaa atagccaccg taagcgggag gtattttgct
     1981 atggaccggg acaagcgttg ggagcgcgtc cagctcgcct ataatgccct tgtccgggga
     2041 ataggggaga agtccaccga tgccctggcc gcggtggaac agtcctacca ggccggcatc
     2101 accgacgaat tcataaagcc gacggtcatc gtcgccagcg atcagcagcc gctggccttg
     2161 ataaaagatg gggactacgc cctctgtttc aatttccgca ccgaccgctg ccgggagata
     2221 accgaagtcc tcacccagca ggatctcccc gatccgggga tgaaaaagct ctccctgcac
     2281 tacaccacca tgacccaata cgacgccaaa ttcaaaggcg taaaggtcat cttcgaaaat
     2341 gacgacctga agaacaccct tggagagatc at
//
LOCUS       DATGFG010000412         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1406012, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000412 DATGFG010000000
VERSION     DATGFG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406012"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /gene="vgrG"
                     /locus_tag="VKQ52_07690"
     CDS             <1..1001
                     /gene="vgrG"
                     /locus_tag="VKQ52_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HLZ87104.1"
                     /translation="ESGNLKAPDLADAIHVSEENLWHGGDLGDEELKSWGNAKLLKSR
                     LAKICGRVSIKGLNGARPGQVVRLKGFGKRFNGPVLVTGITQHYDQSIWETELQFGLA
                     AGWFYQREDISDKPASGLVPGVNGLQPGIVIQLENDPDKQHRVKIQLPLVDGHEGIWA
                     RVASLDAGDGRGSFFRPEVKDEVVVGFFNDDPRYPVILGMLNSSAKPAPIEAKDVNHE
                     KGFVTRSKMKFIFNDEKKTVCLETPKGKKIEIDEENDAIVLSDQHRNKISLTADGIIL
                     ESGKDIQLKAASGEIKLEALNIEQKADAQFSAEGNAGAALKSSGQTVVKGAIVNIN"
     gene            1013..1303
                     /locus_tag="VKQ52_07695"
     CDS             1013..1303
                     /locus_tag="VKQ52_07695"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HLZ87105.1"
                     /translation="MPPAARITDMHTCPMVSGLVPHVGGPILPPGSVNVLVGGLPAAS
                     VGDMLVCAGGPDSIVKGSATVLINNKPAARMGDTTAHGGIIVLGCLTVNIGG"
     gene            1307..1723
                     /locus_tag="VKQ52_07700"
     CDS             1307..1723
                     /locus_tag="VKQ52_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HLZ87106.1"
                     /translation="MSDKSKAFMGRGWAFPVEFHNEGSVRMLSGEEDIQNSLEVLFST
                     QVGERIMQPDYGSELGSFVFTPLNKSTITYMKAIISDAILFYEPRIILEDILIEPSKD
                     RPGVLEINLSYRVSATNNRYNYVYPFYHQEGTNLER"
     gene            1785..>2484
                     /locus_tag="VKQ52_07705"
     CDS             1785..>2484
                     /locus_tag="VKQ52_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87107.1"
                     /translation="MNSHLKIFSRLFKQEGTARDDRYLPALHPENIAIDDRQLKDLLL
                     YLQEYSGHLRFIPGSERMERDGDAADLIPGEESDNFNGQKDRPAQSWKEYLQMDIAVL
                     LADIAAYPVEQTGRLFVELQKNFDMEPDGRSFNLLLDHTYAVFERLDRWYASAIKEQT
                     DSKLKRDLRLYIQSYLSKKFADLRQIRLHAKSLLEGEEITLTKEHALLNVHKVWGEMN
                     SGDRSYGEHVFSGKD"
BASE COUNT          672 a          599 c          727 g          486 t
ORIGIN      
        1 gggaaagcgg taacctgaag gcgcccgatc tggcggacgc tattcatgtg tccgaagaaa
       61 acctttggca tggcggggat ctgggagatg aagagctgaa atcatggggg aatgcaaagc
      121 tgctgaaaag caggctggcc aagatatgcg gcagggtcag cataaaaggg ctgaacgggg
      181 cccggccggg ccaggtcgtc cggctgaaag gctttggcaa gcggtttaac gggccggtgc
      241 ttgttaccgg catcacgcag cattacgacc aatcaatctg ggagactgag ctgcaattcg
      301 gattggctgc cggctggttc tatcagcggg aagatatctc cgacaagccc gcttcgggac
      361 ttgtaccggg tgtcaacggc ctgcaaccag gcatcgtcat ccagttggaa aatgatcccg
      421 acaagcaaca ccgggtaaag atccagctgc ccctggtaga cggtcatgag gggatctggg
      481 ccagggttgc cagccttgat gcaggagatg ggcggggaag ctttttcagg ccggaggtga
      541 aggatgaagt cgtcgtcggg ttcttcaatg acgacccgcg ctatccggtg atcctgggaa
      601 tgctgaatag cagcgccaaa ccagctccga tagaagccaa agacgtaaac catgagaagg
      661 ggtttgtgac gaggagcaag atgaaattca tcttcaatga cgagaaaaag acggtatgcc
      721 tcgagacccc caaggggaaa aagatcgaga tcgacgaaga aaatgatgcg attgtcctgt
      781 cggaccagca tcggaataaa atatccctta ccgcggatgg gatcatcctg gaaagcggaa
      841 aagatataca gctgaaagcc gcatccgggg agataaaact ggaagcgctc aacattgagc
      901 agaaggccga cgcgcagttc tccgcggaag ggaacgccgg ggctgccctg aagtccagtg
      961 gacagaccgt agtcaaaggt gccatcgtga atatcaatta aaatcaaact atatgcctcc
     1021 ggccgcaaga attaccgata tgcatacctg ccccatggta tcggggttag tccctcatgt
     1081 aggcggaccc atcctgcctc cgggcagcgt aaatgtcctg gtcggcggtc ttcccgccgc
     1141 ttcggtggga gatatgctgg tctgtgccgg aggcccggat agcattgtga aggggtctgc
     1201 taccgtgctc atcaacaata agccggctgc caggatggga gataccaccg cgcatggagg
     1261 tattatcgtg ctgggctgcc tgaccgtaaa tatcggagga taaatcatgt cagataaatc
     1321 aaaagccttt atgggtcggg gctgggcctt ccccgtcgag ttccacaacg aagggtctgt
     1381 acggatgctt agcggtgagg aggacataca gaatagcctg gaagtgctat tctccacaca
     1441 ggtgggcgaa cggattatgc aacctgacta cggatcggag ctcggctcct ttgtctttac
     1501 gccgctcaat aaaagcacga tcacctatat gaaggcaatc atcagcgacg caatcctgtt
     1561 ctatgaaccc aggatcatcc tggaagacat attgatagag ccctcgaaag accggccggg
     1621 cgtattggag atcaatttaa gttaccgggt gtccgcgacg aataacaggt ataactacgt
     1681 atatcccttc taccaccagg aggggacgaa cctggaaagg tagccgacac agatgtggaa
     1741 aaccagcccg gaggccgcga aagcttcctt aaaaaaatac aacagtgaac agccatttaa
     1801 aaatattcag ccgcctgttc aagcaggaag ggacggcgag agatgacaga tacctgccgg
     1861 cactgcatcc tgaaaatatt gccatcgacg accgtcaatt gaaggacctg ttgttgtacc
     1921 tgcaggaata ttccgggcat ctccggttta ttcccggatc ggaaaggatg gagcgtgacg
     1981 gtgatgcggc ggatctcatc ccaggcgaag agtcggataa cttcaatggg cagaaggatc
     2041 gaccagcgca gagctggaag gaataccttc aaatggatat cgccgtatta ctggccgata
     2101 tcgcggccta cccggtagag cagaccggac gcctgttcgt ggaattgcaa aagaacttcg
     2161 acatggaacc ggacggcagg agcttcaatc tgcttctgga ccatacttat gccgtctttg
     2221 aaagactgga caggtggtat gccagcgcca taaaggaaca gacggacagc aaattaaaaa
     2281 gagacctgag gttatacatt caatcctacc tgagcaagaa gtttgctgat ctcaggcaga
     2341 tccggctgca tgcaaagagc ctgctcgagg gagaggaaat caccctcacg aaagaacatg
     2401 ccttgctaaa tgtgcacaag gtatggggcg aaatgaattc cggcgaccgg tcctatggcg
     2461 agcacgtctt tagcgggaag gatg
//
LOCUS       DATGFG010000413         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1485645, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000413 DATGFG010000000
VERSION     DATGFG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485645"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VKQ52_07710"
     CDS             <1..249
                     /locus_tag="VKQ52_07710"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF014699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HLZ87108.1"
                     /translation="PGVKREEIPAELVTSSGSGLDPDLSPAGARIQIARIAAARHLSP
                     DKLKDLVDRYTDGPLLGLFGPAHVNVLRLNLALDRIGH"
     gene            260..1384
                     /locus_tag="VKQ52_07715"
     CDS             260..1384
                     /locus_tag="VKQ52_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HLZ87109.1"
                     /translation="MPDKDESVQHFLELIRQSRRGKFKIYIGMSAGVGKTYRMLQEAQ
                     TLLRNGVDVKIGYIETHARKETEALLEGLPVLPRRRLFYKGKELEELDVQGVINLHPE
                     VVVVDELAHTNIEGSKNEKRWQDVMDILDAGINVISAVNIQHIESLNEEIRNISGIEV
                     AERIPDSVLKEADEVVNIDLTADELITRLKEGKIYTNDKIAVALKNFFQPEKILQLRE
                     LALKEVASQVERKIETELPRGSHPRAERFLACIGSNHETARKVIRKTARLASYYCSRW
                     WLLYVQTPKEDGDKIGLAAQRHLINNFKLATELGAEVIRLKQGNIAKGIMETAENHAI
                     TTICIGKPHLSLYNVIMSTAVFNQLLNKLAASDIDVVILS"
     gene            1397..>2482
                     /locus_tag="VKQ52_07720"
     CDS             1397..>2482
                     /locus_tag="VKQ52_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HLZ87110.1"
                     /translation="MSMRLKTKLSLGLIFLFVVIVLFGVLGLFYINRLSDEGRLVLKN
                     NHESLVYCNRMMEALENLRTDKNALATFQENLHKQQGNITEVGEKEATEELTTNFNEL
                     LADPSDASNYTDIRKSINKIQELNELAILRKNAVAQKTADSAKDILTILFTILTLVAF
                     TFIFNLPGIISGPISSLAEGIRAIAAKDYKKRIYLRQGDEFGELAEAFNTMAQKLDEY
                     ESSNLAQIKFEKSRIETIINQMRDGIIGLDEKRNILFLNAVAEKLLGLKESGIAGHYA
                     PDVALSNDLMRTLLRDETEKKDLKIYADNKESYFSKDVLEVTNNSAVIGQVIVLRNIT
                     LFHELNEAKTNFIATVSHELKTPISSIK"
BASE COUNT          648 a          615 c          736 g          483 t
ORIGIN      
        1 ccgggagtaa aaagggagga gatcccggcc gaactggtaa caagctcggg cagcgggctg
       61 gaccctgatc tgtcgcctgc cggcgccagg atacagatag cccggatcgc cgcggcccgg
      121 catctgtcac cggataaact aaaagacctg gtggatcgtt atacagatgg tcccttgctg
      181 ggtctgtttg gccccgcaca tgtaaatgta ctgcggttga acctggccct cgacaggatt
      241 ggacattgaa atacaaccta tgccggataa ggacgaaagc gtgcagcatt tcctggaact
      301 gatccgtcaa tcgcggcggg gcaagttcaa gatctacatc ggcatgagcg ccggggtggg
      361 gaagacctat cgcatgctgc aggaagcaca gaccctgtta cgcaatgggg tcgatgtaaa
      421 gatcggctat atcgagaccc atgcccgtaa ggagaccgaa gcgttgctgg aggggttgcc
      481 ggtgttgccg cggcgcaggc ttttctataa gggaaaggaa ctggaagaac tggacgtcca
      541 gggggtgatc aacctgcacc ccgaggtcgt ggtcgtcgac gaattagcgc ataccaatat
      601 cgaaggcagc aagaacgaga aacgctggca ggatgtcatg gatatcctgg atgcggggat
      661 caatgtgatc agcgccgtca atatccagca tatcgaaagc ctgaatgagg agatccggaa
      721 tatctccggc atcgaagtgg ccgaacgcat cccggacagc gtcctgaagg aggcggatga
      781 ggtggtgaat atcgatctga cggccgatga actgatcacg cggctgaaag agggaaagat
      841 atacacgaac gacaagatcg cggtggcgct gaagaatttt tttcaacccg aaaagatcct
      901 gcagttgcgt gaactggcgt taaaggaagt ggccagccag gtggaacgga agatcgagac
      961 ggaactgccc cggggctcgc atccgcgtgc cgagcggttt ctggcctgta tcggcagcaa
     1021 ccacgaaaca gccagaaagg tgattcgtaa aacggcccgc ctggcgagtt attactgcag
     1081 ccggtggtgg ctgttgtatg tacaaacacc aaaagaggat ggggacaaga tcgggctggc
     1141 ggcgcagcgt cacctgatca ataatttcaa gctggctacg gaactggggg cggaggtgat
     1201 ccggctcaag cagggcaata ttgccaaggg gatcatggag acggcggaaa accacgccat
     1261 taccacgatc tgtatcggga agccgcacct gagcctgtac aatgtgatca tgagcaccgc
     1321 ggtgttcaat caattgctga acaagctggc cgcctcggat atcgatgtgg ttatcttatc
     1381 ataattttgc gtcgctatga gcatgagact caaaacgaaa ttatccctgg gactgatctt
     1441 cctgttcgtc gtgatcgtcc ttttcggcgt tctgggatta ttctatatca atcgcctcag
     1501 cgatgagggc cggcttgtgt tgaagaacaa ccatgagtcg ctggtttact gcaaccggat
     1561 gatggaggcg ctggagaacc tgcgaacgga caagaacgcg ctggccacct tccaggaaaa
     1621 cctgcacaaa cagcagggga atatcaccga ggtaggagag aaggaggcga ccgaagagtt
     1681 gacaaccaat ttcaacgaat tgctggccga tccgtcggat gccagcaatt acaccgatat
     1741 ccgcaagagc ataaacaaga tccaggagtt gaacgaactg gccatcctcc gcaagaatgc
     1801 cgtcgcccaa aagacggctg acagcgccaa ggatatcctg acgatccttt tcacgatcct
     1861 gacgctggtg gcctttacgt tcatcttcaa cctgccgggg atcatttccg gaccgatcag
     1921 cagcctggcc gaaggtatcc gggccatcgc cgccaaggac tacaaaaagc gtatttatct
     1981 gcggcagggg gatgagttcg gagaactggc cgaggccttc aacaccatgg cccagaagct
     2041 ggatgaatat gagagcagca atcttgcgca gatcaaattc gaaaagagcc ggatcgagac
     2101 cattatcaac cagatgcggg acggcatcat cggtcttgat gagaaacgga atatcctctt
     2161 cctgaatgcc gtggcggaga agctgcttgg gctgaaggaa tccggcatcg ccgggcatta
     2221 tgcgccggac gtcgccctgt ccaacgatct tatgcggacc ctgctgcggg acgaaacgga
     2281 gaagaaggat ctgaagatct acgcggataa caaggaaagc tatttcagta aggacgtgct
     2341 ggaggtaacc aacaattcgg cggtgatcgg gcaggtgatc gtcctccgga atatcacgct
     2401 ttttcatgaa ctgaatgagg caaagacaaa tttcatcgcc acggtgtcgc acgagctgaa
     2461 gacgccgatc tcttccatta ag
//
LOCUS       DATGFG010000414         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_471594, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000414 DATGFG010000000
VERSION     DATGFG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471594"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VKQ52_07725"
     CDS             <1..265
                     /locus_tag="VKQ52_07725"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87111.1"
                     /translation="LPNLTAGLSQLAFRDSIVQERAWEFAGENGMRWFDIVRLQMLPQ
                     INAARSPLENPINTSNPLSTRYIAPIPFQDMSNDPNWKQNPGY"
     gene            319..2664
                     /locus_tag="VKQ52_07730"
     CDS             319..2664
                     /locus_tag="VKQ52_07730"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HLZ87112.1"
                     /translation="MKHLLPALLVCLTIRALAQTSAHPLTSETHHPSDPNSRAAARLT
                     SAARHPADPISHAATDPLEYIDPTIGNVGILLEPTRPTAQLPNQPVRVYPLRKDALDD
                     RITAFPLTAVSHRLGEAFALHPAIGTISTDSWTDRMPYDRDLEITRPWYYSTYLLNTG
                     ITVEYTPGRFTGFYRFHFPKDATPSILLDKLNGGPATWQFTGNDKLDASETWQGGVKI
                     FAHLEFTAAGPSGATAAQPDPMDAKPAIHFPAGTTVVNCKYAISFISPEQAIKNFHEE
                     IAHPGFEAVEQHARQAWSSIMNRIRVEGGTAAQRRTFYTALYRCHERMVDITEDGRYY
                     SGYSHQVESDPRPFYVDDWSWDTYLALHPLRTILHPAQEEDMLQSYVRMYKQSGWMPT
                     FPVLFGDYACMNGFHSSVTFLDAWRKGLRNFDSNTAYEGMRKNAVEATLLPWRNGPAT
                     ALDTFYRTNGWFPALHPGEKESVPQVHPFEKRQAVAVTLGASYDDWALAQLAKDLGRT
                     ADEQTFAARAHNYANLWNPEKKFFLPKDEKGDWIDIDPKFAGGPGGRDYYDENNGWTY
                     LWQVQEDIPHLIEGMGGKKATEQRLDQLFREDLGRSKQEFWNKFPDATGLVGQYSMAN
                     EPSFHIPYLYNYAGAPWKTQQKIRLLLSTWFPDNVFGIPGDEDGGGMSAFVVFSMMGF
                     YPVTPGVPIYDLASPVFDRITIHLHNGKNLTIVCRNNSRDNKYIQSIRWNGKPLEQVW
                     IRHGDIISGGTLELRMGNTPNEKLGAEPSSFPPSALDLQFK"
BASE COUNT          676 a         1081 c          709 g          563 t
ORIGIN      
        1 cctccccaac ctgacagccg gcctctccca actggccttc cgcgactcca tcgtacagga
       61 acgcgcctgg gaattcgccg gcgagaatgg catgcgctgg ttcgatatcg ttcgcttaca
      121 aatgctaccc caaataaatg cggcccgcag cccgctggaa aaccccatca atacaagtaa
      181 ccctctcagt acccgctaca tcgctcctat tcctttccag gatatgagca acgaccccaa
      241 ctggaaacag aatcccggat attagaaaac aaaaaataaa aaaggatcac ccgcaagggt
      301 ggtccttcca ctattatcat gaagcatctt ctccccgctc tgctggtctg cctcaccatc
      361 cgcgccctcg cacaaacatc cgcccacccg ctcacatccg aaacccacca cccctcggat
      421 cccaactccc gggccgccgc tcgcctcaca tccgccgccc gtcatcccgc cgaccccatc
      481 tcccacgccg ccaccgaccc cctcgaatac atcgatccca ccatcggcaa cgtcggtatc
      541 ctcctcgagc cgacccggcc gaccgcccaa ctcccaaacc aacccgtccg cgtctatccc
      601 ctgcgtaagg atgcgcttga cgatcggatc accgcttttc cccttactgc cgtttcccac
      661 cgcctcggcg aagccttcgc cctccatccc gccatcggta ccatcagcac cgacagttgg
      721 accgatcgca tgccctatga ccgtgacctc gagatcaccc gtccctggta ttactcgacc
      781 tacctcctca acaccggcat taccgtggaa tatactcccg gccgcttcac cggcttctat
      841 cggtttcact ttccaaagga cgctactcct tccatcctcc tcgacaagct caatggcggt
      901 cccgccacct ggcagtttac cggcaacgac aaactcgacg ccagcgaaac ctggcagggt
      961 ggggtaaaga tattcgccca cctcgaattc accgccgccg gcccgtccgg agccacggcc
     1021 gcgcagcctg atcccatgga tgcaaaaccc gccatccatt tccccgcagg caccaccgtc
     1081 gtgaactgca aatacgccat cagcttcatc agccccgaac aggccatcaa aaacttccat
     1141 gaagagattg cacatccagg cttcgaagcc gtcgagcagc atgcccggca agcctggtca
     1201 tcgatcatga accgtatccg tgtcgaaggc ggaacagcgg cccagcgcag gacattttac
     1261 accgccctct atcgctgcca tgaacgcatg gtcgacatca ccgaggacgg acggtactat
     1321 agcggctact cccaccaggt ggagtccgac cctcggccct tctatgtcga tgactggtcc
     1381 tgggatacct acctcgccct ccatcccctc cgcaccatcc tccatcccgc acaggaagag
     1441 gacatgctcc aatcctatgt ccgcatgtac aaacaatccg gctggatgcc caccttcccc
     1501 gtactcttcg gcgactacgc ctgcatgaac ggcttccact ccagcgtcac cttcctcgac
     1561 gcctggcgaa aaggcctccg caacttcgat agcaacaccg cctacgaagg aatgcggaag
     1621 aatgccgtcg aagccaccct gcttccctgg cgtaacggac ccgctaccgc gctcgatacc
     1681 ttctatcgga cgaatggctg gttccccgcc ctccaccccg gcgaaaagga atccgttccc
     1741 caggtacatc cgttcgaaaa acgccaggcc gtcgccgtca ccctcggagc ctcctacgac
     1801 gactgggccc tcgcacaact cgcaaaggat ctgggcagga cagccgacga acaaaccttc
     1861 gccgcccgcg cccacaacta cgccaatctc tggaacccgg aaaagaaatt cttcctcccg
     1921 aaggatgaaa aaggcgactg gatcgacatc gacccaaaat tcgccggcgg ccccggcggc
     1981 cgtgactact acgacgagaa caacggctgg acctatctct ggcaggtaca ggaagatatc
     2041 ccccatctga tcgaaggcat ggggggtaaa aaggctaccg aacaacgcct tgaccagctc
     2101 ttccgcgaag acctcggccg cagcaaacag gaattctgga acaaattccc cgacgccacc
     2161 ggcctcgtcg gccaatattc catggcaaac gaaccttcat ttcatattcc ctacctctac
     2221 aactacgccg gcgcaccctg gaagacccag caaaagatcc ggctcctcct ctccacctgg
     2281 ttccccgaca acgtcttcgg tatccccggc gacgaggacg gcggcggcat gtcggccttc
     2341 gtggtttttt cgatgatggg attctatccc gtgacgcccg gcgtgcccat ctacgacctc
     2401 gctagcccgg tgttcgaccg catcaccatc catctgcata acggcaaaaa tctcaccatc
     2461 gtctgccgga acaattcgcg cgacaacaaa tacatccaaa gcatccgctg gaatggcaaa
     2521 ccgttggaac aagtttggat tcgccatggg gatatcatta gcggtggcac gctggaatta
     2581 cggatgggca acactcccaa tgaaaagctg ggcgccgagc cgtcgagctt tccgccctcg
     2641 gcgctagatt tacagtttaa gtagtccttt gctggaggtg gttcgcggcg ccgcgggaca
     2701 cggcgcgctc gtcgcagcgc gacgagcact acccaaccaa taggattttg gaaaaggggg
     2761 ctggatttct ttcgttgggg attggtaaat tcgatacgtt tgatggtttc gagccctgca
     2821 ggctggaagc ctgcgataca gcagaccaca ggtctgcgct acgcccgaat ccgccgggcg
     2881 gcagacgccg cctgaactcg taggcgagac ccctgcgcta cgggcgtgtc gccagtttat
     2941 gcgactgccc cctgagcgcc gtggaagtta ttttctcacg agctcatgtg ttgcccgcaa
     3001 gtgcggtgtc caaccgcggg gcaccattt
//
LOCUS       DATGFG010000415         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1667322, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000415 DATGFG010000000
VERSION     DATGFG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667322"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VKQ52_07735"
     CDS             <1..311
                     /locus_tag="VKQ52_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLZ87113.1"
                     /translation="HIIGELNDNYVKLAKLSGEFVWHSHEHEDELFIVLGGTLMMDFR
                     DKPTVATGPGEILIVPKGVEHRPWTKDAEVTIMLIEPKATKHTGDIVTDRTVQQLEWI
                     "
     gene            complement(371..1201)
                     /locus_tag="VKQ52_07740"
     CDS             complement(371..1201)
                     /locus_tag="VKQ52_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87114.1"
                     /translation="MKSNGPFLINITVSLLLGLFAIAGCYTESNLSTQIGAAYPALPD
                     STMFVLLPKVATAHIDGVKIGKVIYNEGLLSTISWTHDRIIDNLRSTALQHGANLAKA
                     TVYVHKTQRTSEYISADLYLVREIQRYNYEDEIEWPSHRTLSFDDFKFSGPILSDPSI
                     GQSKGTYELGTIAKFRCQTSWINRTSRDSVALLLHEQGLFDLCEIYCRQYRDWIRKGN
                     PQEPFFGKMNKAWMEKRSQYEWQTRHGLDFARQAEWTARINDALLFGSGGSDAEFEVH
                     "
     gene            1484..2284
                     /locus_tag="VKQ52_07745"
     CDS             1484..2284
                     /locus_tag="VKQ52_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLZ87115.1"
                     /translation="MPVTHIRGIRLAYDDLGQPNGKPLVFIHGHPFNRTMWTEQVTHF
                     SNNYRLILPDLRGYGETEVTTPRVLLDEMALDIIHLLDELKIEKAVFIGLSMGGQIVL
                     DCYRLISRRFLALAIVDSDARGETPESAARRIEIAEQILQTGMIKHTDETIHKYIAKT
                     SMTNPAVYTPLYKMMTTTKPEGAAAAHKGRALRRDHTAILANIVVPALIVVGSEDFFT
                     PLPIARLMSDNIPGARLAVIDNTGHLPNMESPAEFNKVLGEFLTHLPE"
     gene            2457..>3703
                     /locus_tag="VKQ52_07750"
     CDS             2457..>3703
                     /locus_tag="VKQ52_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87116.1"
                     /translation="MFRNYFLTAWRNLARSRAYSALNILGLSIGMAVALLIGLWVVYQ
                     YSFDRFLPNSTEVYQAHIRFDRNGEPSEMMATPLPLSAELRKEIPEIKYAAVTDWMGN
                     HDLLVGNKKIFLPGAQAEEEFFKIFPYPILKGTLSGALDQPYSIVLTESTAHSLFGDV
                     DPIGKPVRIDNQNNLIVKAVIRDLPPNSTITLNYVIPFSYNILANDWVKQASEENNWN
                     RKSFQTFVSLQPHMDWKQLQPKLSALVKKYAPDDYEKGKVEVFFHPMTDWHLYSDFKA
                     GRQDGGFIEYVRLFSVIGVLVLLIACINFMNLATARSEKRAREVGVRKVLGSQRRDLI
                     VQFLVESLVITLVAAFFSLLLVQLAIPAFNNLTNASIRIPYDSPLFWSVMAIYIFGTG
                     LLAGSRPALYLSSFRSVKVLKGPL"
BASE COUNT          868 a         1273 c          791 g          771 t
ORIGIN      
        1 cccatattat cggcgaactc aatgacaact atgtaaagct cgcaaagcta agcggcgaat
       61 tcgtctggca ttctcacgaa cacgaagacg aactcttcat cgtcctcggc ggcaccctta
      121 tgatggactt ccgcgacaaa ccgaccgtcg ccaccggccc cggcgagata ctcatcgtcc
      181 ctaaaggcgt cgagcatcgc ccctggacaa aagacgccga ggtcacaata atgctcatcg
      241 aaccaaaggc cacgaaacac accggcgata tcgtcaccga ccgcaccgtc cagcaactcg
      301 aatggatctg agtcccgata tcttctatac gcatacatcg ctttgtcaga gcggcggctg
      361 gaccgtggag ctagtgcacc tcaaactctg cgtcggaacc gcccgatcca aatagcaacg
      421 catcgttgat cctcgcagtc cattcggcct gccgggcaaa atcaaggcca tgacgggtct
      481 gccattcata ctgggatcgt ttctccatcc aggctttatt catctttcca aagaaaggct
      541 cctgcgggtt tccctttcgt atccaatccc ggtattggcg gcaatagatc tcacacagat
      601 cgaaaaggcc ctgttcgtgc agcagcaacg caacggaatc tctggacgtc ctgttgatcc
      661 agctggtctg acaacggaat tttgcgatgg tgccgagttc gtatgtcccc ttactttgcc
      721 cgatcgacgg gtcggagagg atcggcccgg agaatttgaa atcgtcaaaa ctcagtgtgc
      781 gatggctcgg ccactcgatc tcatcctcat agttatagcg ctgtatctcg cgaaccagat
      841 agagatcggc ggatatatat tcactggtgc gttgggtctt atgcacgtac acggtcgcct
      901 tagccaggtt ggctccatgt tgcagcgccg tactccgcag gttgtcgatt atccggtcat
      961 gtgtccagga gatggtggaa agcagcccct cgttgtagat gaccttgccg atcttcacgc
     1021 cgtcgatatg cgctgtagct acttttggaa gcaggacaaa catggtcgaa tccggaaggg
     1081 cagggtaggc tgcaccgatt tgcgttgata agttgctttc tgtataacag ccggctatag
     1141 caaaaagccc caacagaagg ctgacggtga tgtttataag gaaggggccg ttacttttca
     1201 tttccctgga ggttgcatac aaaagtagag caccaacctc cggtactgtt gtaatcttac
     1261 gttaacgctt tcctatcgac ttgttaatcc attgacgatc tctccgcaag aggcccctgc
     1321 catcgcccca actcgaagca atctacgttg agccaaaccg ggatactagc tccccatgcc
     1381 gccccatctg atctacccca aaaactaaaa tctgcatctg ttccggcgcc ccctttcccg
     1441 ctacctttgc cgcgcgaaca accgcgacaa acaaaatcac atcatgccgg taacccacat
     1501 ccggggcatc cgcctcgcct acgacgacct cggtcaaccc aatggcaaac ccctcgtctt
     1561 catccacggt catcccttca accgcaccat gtggaccgaa caggtgacgc acttttcaaa
     1621 caactaccgc ctcatcctcc ccgacctccg cggctatggc gaaacggagg tgacgacacc
     1681 ccgcgtcctg ctcgacgaga tggcactcga catcatccat cttctcgacg aactgaagat
     1741 agaaaaggcg gtcttcatcg gtctctcgat gggtggtcag atcgtcctcg attgctatcg
     1801 cctcatctcg cggcgcttcc tcgccctcgc cattgtagac tccgacgccc gcggcgaaac
     1861 ccctgaatcg gcggcacgcc gcatcgaaat cgccgagcag atcctgcaaa caggcatgat
     1921 caaacacacc gacgaaacga tccataaata catcgcaaaa acctcgatga ccaaccccgc
     1981 cgtctacacc cccctctata aaatgatgac caccacaaaa cccgaaggcg ctgccgcggc
     2041 ccacaagggt agggccctcc gccgcgacca caccgcaatc ctcgccaaca tcgtcgtccc
     2101 cgctctcatc gtcgtcggct ccgaagactt cttcaccccc ctgcccatcg cgcgtctcat
     2161 gagcgacaac atccccggcg cccgcctcgc cgtcatcgac aacaccggcc acctcccaaa
     2221 catggaatcc cccgccgaat tcaataaagt gctgggagaa ttcttaactc atctgccgga
     2281 atgattggct tctatagcta ttccacgacg agacacccgc gattttacac cgtacaccaa
     2341 atgtatcaaa atcgcacaac ctccccccat cgccccaaac taacccattg atccccaacg
     2401 cgaaatattt ccggcacccc tttcgcccac ataatcctaa ccaacgaagt cccaccatgt
     2461 tccgtaacta cttcctcacc gcctggcgca acctcgcccg cagcagagcc tacagcgccc
     2521 tcaacatact cggcctctcc atcggaatgg ccgtcgccct cctcatcggc ctctgggtag
     2581 tataccaata ctccttcgac cgtttcctcc ccaactctac cgaggtctac caggcccaca
     2641 tccgcttcga ccgcaacggc gaacccagcg agatgatggc caccccgctg cccctctccg
     2701 ccgaactccg caaagagatc ccggaaataa aatacgccgc cgtcaccgac tggatgggca
     2761 accacgacct cctcgtcggc aacaaaaaga tcttcctccc cggcgcccag gccgaagaag
     2821 aattcttcaa aatctttccc tatcccatcc tcaaaggcac cctcagcggc gccctcgatc
     2881 agccctattc catcgtcctc accgaatcca ccgcgcactc cctcttcggc gacgtcgacc
     2941 ccatcggcaa acccgtccgc atcgacaacc agaacaacct catcgtaaaa gccgtcatcc
     3001 gcgacctccc gcccaactcg acgatcacgt tgaattatgt tattccgttc agctataata
     3061 tccttgccaa tgactgggta aaacaggcct ccgaagaaaa taactggaac cggaagtcct
     3121 tccaaacctt cgtcagcctg caaccgcata tggattggaa acaactccag ccaaaactct
     3181 cggccctcgt aaaaaaatac gcccctgatg actatgaaaa gggaaaggta gaggtcttct
     3241 ttcaccccat gaccgactgg catctctact ccgacttcaa agccggccgg caggacggcg
     3301 gcttcatcga atacgtccgc ctcttcagcg tcatcggcgt cctcgtgctc ctcatcgcct
     3361 gtattaactt tatgaacctg gccacggccc gcagtgaaaa acgcgcccgg gaagtcggcg
     3421 tccgcaaggt cctcggttcg caacgtcgcg acctcatcgt ccaattcctc gtcgagtccc
     3481 tcgtcatcac cctcgttgcc gccttcttct ccctcctcct cgtccagctg gcgatccccg
     3541 ccttcaataa cctcaccaac gccagcatcc gtattcccta cgacagccct ctcttctggt
     3601 ccgtcatggc aatctatatt tttggcaccg gcctcctcgc cggcagccgt cccgccttat
     3661 acctcagctc ctttcgatcg gtaaaagtcc tgaagggccc tct
//
LOCUS       DATGFG010000416         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1048464, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000416 DATGFG010000000
VERSION     DATGFG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048464"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="VKQ52_07755"
     CDS             <1..1358
                     /locus_tag="VKQ52_07755"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HLZ87117.1"
                     /translation="VINIAYTVRKKKSFIASDFGILLANTCLYFAAGLYLIAQLGLDR
                     YKGLYCAAMGIFHLGATWFLFRRNTVDKNVLYLLLGLTLSFVSLTAPIQLHGHYITLF
                     WASEAVLLYWLFLRSQMELIRVAAFLVWGAMLVSLTMDWLHIYGRGILTISPVKPESL
                     PIVFNKGFMTGLYAALSCWALFYLRQGTLWTKAALLTGGAVFFIAGLLEIHFQFTFYY
                     PAFELWVLYALLFTYVCVFVFARITEGLSSADLRPVGAGLLAAGIVVYLCSLVSVFHI
                     QQYFLETGRAGGHFITHWAGDLLTGLILARLIVRGRKEKFAVEPVLFTWLICIVTVTF
                     LSAEGQLLVNVLFFSRENGLDDLARVYNKTGLPILWGLCSFAFMWLGMRHKFKPLRIV
                     SLALFTLTLLKLFLFDIRDIPIAGKIAAFFCLGVLLLVVSFMYQRLKKIIIEDEEKRP
                     I"
     gene            1336..2250
                     /locus_tag="VKQ52_07760"
     CDS             1336..2250
                     /locus_tag="VKQ52_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87118.1"
                     /translation="MRKKGRYRWLPAVLLVFVCCRAGAQGVFAWRATADTVSRDGFYQ
                     ILLTPEVVAKCRADLGDLRILGPGNRFVSYVLKDSWEGATAEKDRLTIPGAVMAQKDS
                     SDQHSYVDLRFPEAYEVDWLSFSIRDPVFYKRAAEVFAEGANGAGWTRVTSITLAPTA
                     YMVKIPSVKTRRLRVVVANADNAPLVINGAGGFQAARCLLAYLKAGMVYQVLAGNAQA
                     AAPEYDLKYFTDSLTKTPPTLLLGPTEYAAYVNDHPAAPATKAKTVPGGHSGLLLWSI
                     LLAILILLIYFSVKMARVITQKEQHDRV"
BASE COUNT          427 a          578 c          718 g          613 t
ORIGIN      
        1 ttgtcatcaa tattgcctat acggtacgta agaaaaagtc ctttatcgca tcggatttcg
       61 gcattctcct ggcgaatacc tgtctgtatt tcgcggccgg gctctacctg atcgctcagc
      121 tggggctcga ccgctataag gggttgtatt gcgcggcgat ggggatattc catctcggcg
      181 ccacctggtt ccttttccgg aggaatacgg tggacaagaa tgtcctttac ctgcttttgg
      241 ggcttaccct gtcctttgta tccctgacgg cgcctattca gctgcatggg cattatatca
      301 cgcttttctg ggcctccgag gcggtgttat tgtattggct tttcctgcgg tcgcagatgg
      361 agttgatccg ggtggccgcg tttctcgtgt ggggggcgat gctggtgagt ttgactatgg
      421 actggctgca tatctatggc cggggtatct tgacgatatc gcccgtgaag ccggagagcc
      481 tccccatcgt attcaacaag ggatttatga cggggctcta tgcggcgctg agctgctggg
      541 cgctgttcta tctgcgccag gggacgttgt ggacaaaggc cgccttgctc acgggtggcg
      601 cggtgttttt tatcgccggg ctgctcgaga tccatttcca gtttaccttc tactatcccg
      661 cattcgaact gtgggtgttg tatgccttac tattcaccta tgtttgtgtg tttgtcttcg
      721 ccaggattac cgaagggctg agttcggcgg atctccggcc ggtgggcgcg gggttgctgg
      781 cggcggggat cgtggtgtac ctgtgctcgc ttgtttccgt cttccatatt cagcaatact
      841 ttctcgagac gggccgtgcg ggggggcatt ttatcacaca ttgggccggt gacctgctga
      901 cggggctgat cctggcgcgg ctgatcgtgc ggggaagaaa ggaaaaattc gccgtcgagc
      961 cggtgttgtt cacctggctt atctgtatcg tgacggtgac gtttctgagt gcggaaggtc
     1021 agttgctggt gaatgtcttg ttcttttccc gggagaacgg gcttgacgac ctggcgcgtg
     1081 tatataacaa gacgggtttg ccgatccttt gggggctgtg ctcttttgcc tttatgtggc
     1141 tggggatgcg gcataaattc aagcccctgc ggatcgtctc gctggcgctg ttcacgctta
     1201 cgctgctaaa gctgttcctt ttcgatatcc gggacatccc gattgccggg aagatcgccg
     1261 cgttcttctg tcttggtgtc ctgttactgg tcgtgtcctt tatgtatcag cggttgaaaa
     1321 aaatcattat cgaggatgag gaaaaaaggc cgatataggt ggctgccggc cgttctcctg
     1381 gtatttgtct gctgcagggc cggcgcacag ggtgtttttg cctggcgggc gacggcggat
     1441 accgtaagca gggacggctt ctaccagatt ctgcttaccc cggaggtggt ggcgaaatgc
     1501 agggccgatc ttggcgattt gcgcatcctg gggcccggca accgctttgt ctcttatgta
     1561 ctgaaagact cctgggaggg ggcaacggca gagaaggatc ggcttactat ccccggggcc
     1621 gtcatggcac aaaaagacag cagcgaccaa cacagctatg tggacctgcg gtttcccgag
     1681 gcttacgagg tagactggct cagcttttcc atccgggacc cggtgttcta caaaagagcg
     1741 gcggaggtct ttgcggaagg ggccaatggc gccggatgga cccgggttac ttctatcaca
     1801 cttgcaccaa cggcgtatat ggtgaagatc ccgtcggtga agacccggcg gctgagggtt
     1861 gttgtcgcca atgccgacaa cgcccccctt gtgatcaatg gcgccggggg tttccaggcg
     1921 gcccgctgcc tgctcgctta tctgaaggcg ggtatggtat accaggtatt agccggcaat
     1981 gcacaggcgg ccgcaccgga atacgatctc aaatatttta cggacagtct gacgaagacg
     2041 cctcctaccc tgctgctggg gccgacagag tatgcggcgt atgtcaatga ccatcccgct
     2101 gctcctgcga caaaggcaaa aacggtgcct ggtgggcatt cgggcctcct gctttggagt
     2161 attttattgg ctatattgat ccttttgatt tacttcagtg ttaaaatggc aagggtcatt
     2221 acacaaaaag aacagcatga tcgtgtataa tatcaccagt aaggtgcgat gggagatatt
     2281 ggagggttgg gtcacctggc aggtggaaga aatgatcccc gctatcctga agacgg
//
LOCUS       DATGFG010000417         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1431467, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000417 DATGFG010000000
VERSION     DATGFG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431467"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VKQ52_07765"
     CDS             <1..355
                     /locus_tag="VKQ52_07765"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HLZ87119.1"
                     /translation="LLQGAGARLTRTDYVACPSCGRTLFDLQTTTERIKARTAHLKGV
                     KIAIMGCIVNGPGEMADADFGYVGSGPGKITLYKGKEIVKRGVDSAIAVEELIRLLKE
                     HDAWIEPVETANVNS"
     gene            375..1328
                     /locus_tag="VKQ52_07770"
     CDS             375..1328
                     /locus_tag="VKQ52_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HLZ87120.1"
                     /translation="MNRIDRLHAILTHLQSKRKVTAQEMADRFNISLRTVYRDVKALD
                     ESGVPVIGEAGSGYTIMEGYRLPPVMFTQEEASALLLGAKLAEQFTDGSVKRHFHAAL
                     FKIKAVLRSPDKEYVESLTEHIEILSRYRPDNDSPQQHLSLLQQAIVHKRLVYVHYRS
                     NLKEEDTRRKVEPIGLLYYGSAWHLIGWCHLRNDYRDFRLSRMQGVILEDATFDPTAH
                     PSMKEYIEQIRAESDLQEAVVRFEKRIVKYLQEQKYLNGFVSEEEGDACVRMKFLVSN
                     LTYFARWLLMYTDAVRVEGPEALKVQIRELAEKSSVHHGSY"
     gene            1378..1920
                     /locus_tag="VKQ52_07775"
     CDS             1378..1920
                     /locus_tag="VKQ52_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87121.1"
                     /translation="MLIRTHPFMTVLYTTRQARLNDLPAWADHGSGGRRPVISGVEPE
                     GYLESKPGGATNCGSLSRDLYRYVADLDLLVCGPQYWFYYGVDGRPETRFTLEVALPV
                     QGKIPTALLPYFKQIPAFRCLSSRYEGPWEGIAAQYEKMLQHIKENQLKMSGIYAESF
                     LHIDPDHPDNQITEIQIGLC"
BASE COUNT          483 a          647 c          640 g          441 t
ORIGIN      
        1 cctgctgcag ggcgcgggcg cgcggctcac gcgcaccgat tacgtggcct gcccgtcctg
       61 cgggcgcacc ctcttcgacc tccagaccac caccgaacgg atcaaggccc gcaccgcgca
      121 cctcaagggc gtcaagatcg cgatcatggg ttgtatcgtt aacggaccgg gtgagatggc
      181 cgacgcggac tttgggtatg tgggcagcgg gccgggcaag atcaccctct ataaaggaaa
      241 agagatcgtc aagcgcggtg tggacagcgc tattgcggtg gaggagctca tccgcctgct
      301 aaaggaacac gacgcctgga tagaacccgt ggaaacggca aacgtaaaca gttgacaaac
      361 caatcccatt gccgatgaac cgcatcgaca ggcttcatgc catactcacc catctgcaaa
      421 gcaagcgaaa ggtgacggcc caggagatgg ccgatcgctt taatatcagc ctgcggacgg
      481 tgtaccggga cgtcaaggcc ctggatgagt ccggcgtgcc cgtcatcggc gaggccgggt
      541 cgggctatac gatcatggag ggttaccggc tgccacccgt catgttcacc caggaggagg
      601 ccagcgcgct gctgttgggg gccaaactgg ccgagcaatt cacggatggc tcggtaaagc
      661 ggcatttcca cgcggccctg ttcaagataa aggccgtgct tcgctctccc gacaaggaat
      721 atgtggaaag tcttaccgaa catatcgaga tcctttcccg gtacagaccc gacaatgatt
      781 cgccccagca acatctttcg ctcttgcaac aggccatcgt ccataagaga ttagtatatg
      841 tccattaccg gtcgaacctg aaggaagagg atacgcgccg gaaggtggag ccgatcgggc
      901 ttttgtatta cgggtccgcc tggcatctta tcggctggtg tcatctgcgg aatgactacc
      961 gggatttccg gctgagccgg atgcagggag tcatcctgga agacgcgacc ttcgatccca
     1021 ccgcccatcc ttcgatgaag gagtatatcg aacagatcag ggcggagtcc gacctgcagg
     1081 aggccgttgt gcggtttgag aaaagaatcg taaaatacct gcaggagcag aaatacctga
     1141 atggtttcgt ctcggaagaa gaaggcgatg cctgtgtccg gatgaaattt ctcgtctcca
     1201 acctgaccta ttttgcccgc tggctgttga tgtatacgga tgcggtgagg gtcgagggcc
     1261 ccgaggcctt aaaggtccag ataagggaac tggccgaaaa atcttccgta catcatgggt
     1321 cctactgaca tactgctgtc actttagggg tgttcctttg gggaaaccaa ctattgtatg
     1381 cttattcgta cgcatccctt catgaccgtc ctttatacca cccggcaggc ccggcttaat
     1441 gatcttccgg cgtgggcgga ccatgggtcc ggcggccgaa ggcccgtcat tagcggggtt
     1501 gagccggagg gctatcttga atcaaagccc ggaggcgcaa ccaattgtgg cagcctctcc
     1561 cgcgatcttt accggtatgt ggcggatctc gaccttctgg tatgcggtcc gcaatactgg
     1621 ttctactatg gtgtcgacgg ccggcccgag acccgcttta cgctcgaggt ggcgcttccc
     1681 gtgcagggga agatccccac ggccctcctg ccctatttca aacaaatccc cgccttccgt
     1741 tgtctttcca gtcgttatga aggcccctgg gaggggatcg ccgcgcaata tgagaagatg
     1801 ttgcaacaca taaaagagaa ccagctgaaa atgagtggca tttacgcgga atcttttttg
     1861 catatcgatc cggatcaccc ggacaaccag atcaccgaga tccagatcgg gctttgctga
     1921 cggggggccc ggatggcggc ccggacccag gatgccggcc cagaatccag ccaggatgcc
     1981 gcccaggatc gcgcccgggg cccggatgcc gcggtgttcc gggatgccgc cccggatgcc
     2041 agccccggcc ctcagatctt gccccgggcc gcagatgccg cccctgacac cagatcccgc
     2101 gccggacccc ggaccccgga ccccggaccc cggacccccg gaattattaa ccaataactg
     2161 ttttaaatct ttaatatgaa aaaaagcacc tgcgcagcct ttatttttga c
//
LOCUS       DATGFG010000418         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_266328, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000418 DATGFG010000000
VERSION     DATGFG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266328"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VKQ52_07780"
     CDS             <1..182
                     /locus_tag="VKQ52_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ87122.1"
                     /translation="IHARLLVEAKRHLLHTELSVKEVADGLGFEDPAYFNRWFKRMTG
                     NTPIGYRAEIRAIYR"
     gene            362..1090
                     /locus_tag="VKQ52_07785"
     CDS             362..1090
                     /locus_tag="VKQ52_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ87123.1"
                     /translation="MNTSTAFITGANKGIGLETARLLAAAGYHVLLGSRDRARGEQAA
                     AALRSAGYDRVESVVVDVSKEESVQAAFAEVAARVPRLDVLVNNAGIPGSFQQLPSAA
                     EDENLKKVFETNFFGVIRMIRVFMPLLRKSEQPRIVNVTSDLASLTLHSDPQWVYYPI
                     KSPAYGPSKTALNAYTVALAYELRDTPFKVNAVNPGYTSTDFNQHRGTKKVEEAAKII
                     AEYAMLPADGPTGKFVSDYGESPW"
     gene            complement(1160..1360)
                     /locus_tag="VKQ52_07790"
     CDS             complement(1160..1360)
                     /locus_tag="VKQ52_07790"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HLZ87124.1"
                     /translation="MQHTTSHDLRFKGNWNELKGKIKQKYANLTDNDLLYEQGKEDEL
                     LGRLQKKTGKTKQELKDWIDTL"
     gene            1705..>2434
                     /locus_tag="VKQ52_07795"
     CDS             1705..>2434
                     /locus_tag="VKQ52_07795"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HLZ87125.1"
                     /translation="MKALCIGAAAFLLFSFTPFKTIADIPPNAVASVSVVAVPSAVSS
                     PSAVGVNSAGALRSARVAVSLADSARFDLLYENLDLQGLALSREAYHRAVLGFLTMQI
                     TGLVRNPDVLSIVDFSLPSSQKRLFVIDMLNGRLLFNTFVAHGRNSGALMATRFSNRT
                     NSYMSSLGFYLTGDPFFGSHGYSLRLQGIERGWNDHAFNRRIIMHPADYVSEEHIQQW
                     GYLGRSEGCPAIPEELNAPIIDEIK"
BASE COUNT          471 a          561 c          786 g          616 t
ORIGIN      
        1 atatccatgc gcgcctcctg gtggaggcca agcgtcacct gttgcatacg gagttatccg
       61 tcaaggaagt ggcggatggg ctggggtttg aggacccggc ctatttcaac aggtggttca
      121 agcgaatgac cgggaatacg ccgattgggt accgggcgga gatccgggca atataccggt
      181 gaggcggggg cgatgcgtgg cgggagaaga ggcgatgggt gcccggagaa ggaatgggcg
      241 atgcgtggcg ggggggagtg aaggtcgatg tgcggccggg gggaacgaaa tccgggaaat
      301 gtaccgttca tgcccggcaa tgtgcaaggt ggttttgggg cgccgcgagt agttttgctt
      361 catgaataca tcaacagctt ttatcacagg ggccaataag ggtattgggc tggagaccgc
      421 gcgcctgctg gcggcggcgg gttatcatgt tcttttgggt tcacgggacc gggcacgtgg
      481 ggaacaggcg gcggcggcat tacgatcggc gggatatgac cgggtggagt ccgttgtcgt
      541 cgatgtcagc aaggaagaat cggtgcaagc ggcctttgcc gaggttgcgg cgcgggtccc
      601 acggctggac gtactggtga acaatgccgg tattccgggg agttttcagc aacttccgtc
      661 ggcggccgaa gatgagaatc tgaagaaggt gttcgaaacg aatttctttg gggtgatacg
      721 gatgatccgg gtgtttatgc cgctgttgcg caagtcggag cagccgagga tcgtgaatgt
      781 gacgtccgat ctggcgtcgc tgacgctgca cagtgatccg cagtgggtat attacccgat
      841 caagagcccg gcgtatggcc cttcgaagac ggcgctgaac gcctatacgg tggcgctggc
      901 ctatgaatta cgggatactc ccttcaaggt aaatgcggtg aacccggggt atacttcgac
      961 cgacttcaat caacatcgtg gaacgaaaaa ggtggaggag gcggcgaaga tcattgcgga
     1021 atatgcgatg ttgccggccg atgggccgac ggggaagttc gtcagtgatt atggcgagtc
     1081 gccgtggtaa aaaacaaggg ccatgaagag gatattgttt cccttcatag ccctacgctg
     1141 ccatgcagca tgctcctttc tatagggtat cgatccagtc tttcaactcc tgtttggttt
     1201 ttccggtttt cttttggagg cggccgagca gttcatcttc ctttccttgt tcgtacaaca
     1261 gatcgttgtc cgtcagattg gcatatttct gcttgatctt gcctttcaat tcgttccaat
     1321 tacctttaaa tctcagatcg tgacttgtag tgtgttgcat atatttcaat ttttaatgtg
     1381 acttacgtat ggggcggcgt ggggtgggtt agaatgtgca attgatgccc caatcggggc
     1441 agggaggtta caggataaac cggtggaatc gcgctgatga gcggccgttg ttgatactgc
     1501 agaaaaaatt gtacccgggg aggcgtgggc gccccgggag gtgtggggct cggagggtgt
     1561 agggcttggg aggtttgcat ggttcgggag ggggcttagg gaagggtaag ggttttgcgt
     1621 taaaattttt ataaagccgc ggggatgggg gcgtggcacc gggggacacc ggtatctttg
     1681 tcgggattta aaattcttcc tgtgatgaaa gcattgtgca tcggcgcggc cgccttttta
     1741 ttatttagct ttactccttt taagacgatc gccgatattc cccccaatgc tgttgcatct
     1801 gtttccgttg tcgccgtgcc gtcggccgtt agttcgcctt ccgctgtagg tgtaaattcc
     1861 gccggtgcgc tgcgttccgc cagggttgct gtttcgctgg ccgactccgc ccggtttgac
     1921 cttttatacg agaatctcga tctgcagggg cttgcgctct cgcgcgaagc ctatcatcgg
     1981 gccgtactcg gctttttgac gatgcagatc accgggcttg tccgtaatcc cgacgttttg
     2041 tcgatcgtcg atttttccct tccgtcctcc cagaagcggc tttttgtcat cgacatgctg
     2101 aatggccggc ttttgttcaa tacgtttgtg gcgcatggcc ggaattcggg tgcgttaatg
     2161 gcgacacgtt tttccaaccg caccaatagt tatatgagca gtctcggttt ttacctcacc
     2221 ggtgatccgt tcttcgggtc acatggctat tcgctgcggt tacagggcat cgagcggggc
     2281 tggaatgacc atgctttcaa ccggaggatc attatgcatc ccgctgatta tgtcagcgag
     2341 gagcatatcc agcagtgggg atacctgggt cgcagcgagg gttgtcccgc catcccggag
     2401 gagctgaatg cgccgatcat cgacgagatc aaag
//
LOCUS       DATGFG010000419         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1276708, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000419 DATGFG010000000
VERSION     DATGFG010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276708"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VKQ52_07800"
     CDS             <1..636
                     /locus_tag="VKQ52_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ87126.1"
                     /translation="EIVLPDSTRVYLGPAGTLRYGQNDDHNRVVQLEGEAYFVVRHDR
                     LRSFTVVAGALSIRDIGTEFNIRYYPGEPTMAIAVASGKVQVLRTPAATQNAAAPPPA
                     ANSLAALLPGQLLQYDSLTQRSGITDLPDPSAIGAWRKGILSFHRQRLKEVTGELERY
                     YGVRFRYSDHSAENILITTLLDNRNLGDALDIVSLTAGVHFIRQGTSIVVN"
     gene            658..>3761
                     /locus_tag="VKQ52_07805"
     CDS             658..>3761
                     /locus_tag="VKQ52_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ87127.1"
                     /translation="MKKLLLLFIVLSAIPDLLPAQDPGKKATVRFRHASLRQAFTELE
                     KQYGLNFSYNENNLALYAKEIDFQLKDVTASQVLDKIFAGSPLTWSFKGNLVVLTADP
                     NYRKKPGNSHALSEVLTGTITDGETTEPLPGVTIKAGDQAVQTDKEGRYSLPLTAGSY
                     SVTISHIGYAAREMHGVEISSATPTVVNLTLYKAGADLGEVVVVGYGRQSRKLLTSAI
                     STVQNSEFNQGNFNSPAQLLQGKVAGLTIARSGDPNGAPSIVLRGPSTLRPGAAQEPF
                     YVIDEVPGADIRLVSPDDIAAFEVLKDASATAIYGTRASNGVIMITTKKGRQAQTLVS
                     VNSKVSVENVARKIHMMNAGQLKAYLARNSLALDPSDDQGANTDWQEAVTQTAVSQNQ
                     HVSLSGGSNNLTYTATINYFDSKGILRNTGLNRLIGRAAIEQRVLDNHLKLGMSVSTA
                     TSNSDLLPDQSIILYNMLRYLPTVPIKEADGSYTENLQRVQYYNPVSLQNNAWQNLKN
                     KTTLLNGTAQVILPIGFKYDLNLTSQSGQDNSGTYYTSQYTLKTGINGQAYRSSYESS
                     KKMLETFLTFARRIRDHDINVLGGYSWQEDVTGDGFQASNQNFPTDALGYTNIGLGSP
                     IGNYRNDWGVNQYQKLRLISFYTRAKYSFKDRYLLQASLRRDASSAFGVNNRWATFPS
                     ASFAWRAIDESFLKSQSLFSDLKWRISYGITGNSLGFDPLISKVQYSSVGAFYYNGTF
                     VNAIGPSQNANPDLKWEKTAMFNTGVELALLKGRVKAIIEYYDKKTSDLIWYYPVSTI
                     QYFVSTYTANVGSISNKGFELTVDATPVKNASFQWVTSLSLAHNENRLVSLSNNIFKL
                     DSIFEASPGGQGQTGSYVQIIKSGKPVGQFFTFSYAGKNAAGVSQFYDATGKITLAPM
                     NFVDYRYAGNAQPKLQLSWGNTLTWKGFDCNIFVRVFLGAKVMDATLADLNRPDEVTN
                     YNLPVFSTNESSRDISAYIYSNRYVENASYLRLDNVTLGYTFSSFFKGMRSLRLYVAG
                     NNLA"
BASE COUNT          893 a         1222 c          896 g          750 t
ORIGIN      
        1 gagatcgtcc tgcccgacag cacccgcgtc tatctgggac ccgccggtac gctccgctat
       61 ggacagaatg acgaccacaa ccgcgtcgtt caactggaag gagaagccta cttcgtcgtg
      121 agacacgatc ggctccggtc atttaccgtc gtcgccggcg ccctctccat ccgggatata
      181 ggcactgagt tcaatatccg ctattacccc ggcgaaccga ctatggcaat agcggtagcc
      241 agcggtaagg tccaggtatt gcgcacgcca gccgccacgc agaacgccgc cgcaccacca
      301 cccgccgcca actccctggc ggcactcctc cctggccaac tccttcagta cgattccctg
      361 acccaacggt ccgggataac ggaccttccc gacccatcag cgatcggcgc ctggcgcaaa
      421 ggcatcctct ccttccaccg gcagcgcttg aaagaggtaa ccggagaatt ggagcgctac
      481 tatggagtcc gatttcggta cagcgaccat tccgcagaga atatcctgat caccaccctg
      541 ctcgataaca gaaacctcgg agatgctctc gatatcgtat ccctcacggc cggcgttcac
      601 tttatccgcc agggtacgtc catcgtcgta aactaagtcg tatactaagg tacaagtatg
      661 aaaaagttat tgctcttgtt catcgttctg tccgcgatac cggacctcct gccggcccag
      721 gaccccggca aaaaagcgac cgtccgtttc agacatgcca gcctccgcca ggcttttacc
      781 gaactggaaa agcaatatgg cctgaacttc tcctacaacg agaacaacct cgcgctatat
      841 gcaaaagaga tcgatttcca gctaaaagat gtcacggcca gccaggtcct cgacaagatc
      901 tttgcaggca gcccgttgac ctggtcgttc aaaggaaacc tggtagtgct taccgctgat
      961 cccaattatc ggaagaaacc cggcaactcc cacgccctct ccgaagttct cacaggcacc
     1021 atcaccgatg gagagacgac ggaaccctta cccggcgtca cgatcaaagc cggtgatcag
     1081 gccgtgcaga ccgacaagga aggccgatac tcccttcccc tgaccgcggg atcttattcc
     1141 gtgaccattt cccacatcgg ttatgccgcc cgggagatgc atggtgtcga gatttcttca
     1201 gcaaccccca ccgtggtcaa cctcaccttg tacaaggccg gggccgatct gggcgaggtt
     1261 gtcgtcgtcg gttatggccg ccaatcccgc aaactgctca ccagcgccat cagcaccgta
     1321 caaaactcgg agttcaacca gggcaatttc aacagccccg cgcaattgct ccagggcaag
     1381 gtggctggcc ttaccattgc ccgctcgggt gaccccaatg gcgccccgtc gatagtcctt
     1441 cgcggcccct ccaccctgcg tccgggcgcc gcccaggaac ccttctatgt catcgatgaa
     1501 gtccccggcg cggatatccg gttggtatcg ccggacgaca tcgccgcctt cgaagtgctg
     1561 aaagatgcct cggcgaccgc catctatgga acaagggctt ccaatggggt gatcatgatc
     1621 acgacaaaaa aaggccgcca ggcgcagacc ctcgtcagcg tcaacagcaa ggtatcggtg
     1681 gaaaatgtgg cccgtaagat ccatatgatg aacgccggcc aactcaaggc ctacctcgca
     1741 aggaacagcc tggccctcga cccatccgac gaccagggcg ccaataccga ctggcaggaa
     1801 gccgtcaccc aaacagccgt gtcccagaac caacacgtct ccttgagtgg cggctcgaac
     1861 aatctcacct atactgccac aatcaattat ttcgacagca aggggatact gaggaatacc
     1921 ggtctgaacc ggctgatcgg ccgcgccgct atcgagcaac gcgtcctgga caaccatctg
     1981 aagctgggga tgtccgtcag tactgctacc agcaattccg acctgctgcc ggatcagagc
     2041 atcattctgt acaatatgct gcgctatctt cctaccgttc ccataaagga agctgacggc
     2101 agctacacgg aaaatctcca gcgggtgcaa tactataatc ctgtttcgct gcagaacaac
     2161 gcctggcaaa acctaaagaa caagaccaca ctgctcaacg gaacggcgca ggtgatcctc
     2221 cccatcggct tcaaatacga cctcaacctg acctcccaga gcgggcagga caatagcgga
     2281 acctactata cgagccagta taccctgaag acaggcataa atggccaggc ctatcgttct
     2341 tcctatgaga gctcgaagaa aatgctcgag accttcctca cctttgccag acggatacgc
     2401 gaccacgaca tcaacgtact gggggggtac agctggcagg aagacgtcac cggcgatggc
     2461 ttccaggcaa gcaaccagaa cttccccacc gacgcccttg gctacaccaa catcggcctc
     2521 ggcagcccaa tcggcaacta ccgcaacgat tggggcgtca atcaatatca gaagcttcgc
     2581 ctcatctctt tttatacccg cgcaaagtat agcttcaagg accgctacct gctgcaggcg
     2641 tcgctccgcc gcgatgcctc ctccgccttc ggcgtcaaca accgctgggc taccttcccc
     2701 tccgcctcct tcgcctggcg cgccatcgac gaatcctttc tcaaatccca gtcgctgttc
     2761 agcgacctga aatggcgcat cagctatggg atcacgggaa actccctcgg cttcgatccg
     2821 ctcatctcga aagttcaata tagctccgtc ggggcgtttt attacaacgg aaccttcgtc
     2881 aatgcgatcg gcccctccca aaatgccaac cccgatctca aatgggagaa gacggctatg
     2941 ttcaacaccg gtgtcgaact cgccctgctg aaaggccggg tcaaagccat catcgagtac
     3001 tacgacaaaa agaccagcga cctcatctgg tattaccccg tttctacgat ccagtatttt
     3061 gtgtccacct acaccgccaa tgtcggttcc atctccaaca aagggttcga gttgaccgtc
     3121 gacgcaacgc ctgtgaagaa cgcgtccttc cagtgggtga cctctctcag cctcgcacat
     3181 aatgagaaca ggttggtttc actgtccaac aatatcttca agctcgattc catcttcgag
     3241 gcttcccccg gcggccaggg ccaaacgggc tcctatgtac agatcatcaa gtcaggcaaa
     3301 cccgtgggtc agttcttcac cttcagctat gccggcaaga atgcggccgg tgtctcccag
     3361 ttctatgatg ccaccggcaa gatcaccctc gcacccatga acttcgttga ctaccggtat
     3421 gctggcaatg cacagccgaa attacagctc agctggggca acacccttac ctggaagggc
     3481 tttgattgca atatattcgt aagggtcttc cttggagcaa aggtcatgga tgccaccctg
     3541 gccgatctca atcgcccgga cgaggtcacc aactacaatc taccggtatt ctccactaac
     3601 gaatcgtcca gggacatcag cgcctatatc tattccaacc ggtacgtgga aaacgcctcc
     3661 tatctgcgcc tcgacaatgt aacgctgggt tataccttct cttccttttt taaagggatg
     3721 cgcagtctcc gcctctatgt agccggcaac aacctggcca t
//
LOCUS       DATGFG010000420         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_68172, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000420 DATGFG010000000
VERSION     DATGFG010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68172"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VKQ52_07810"
     CDS             <1..805
                     /locus_tag="VKQ52_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ87128.1"
                     /translation="LPTMGLQLVAGRNFHPDIHSDSGNVIINQSMASLMGKAGQVGAL
                     LTYGDTNKRYYHIIGIVKDFLFNDLAGTVTPPLMLDCGREVQGVYNFLTIRLKPGKDL
                     SAQLAKVEAVIKANNPGYPVDYRFVDEQFNDMFESQAHMGALAGVFAVLAILISCLGL
                     FGLAAYTAERRTKEIGIRKVLGASVSGLAALLSIEFLQLVSLSCLIAFPVAWWLMHGW
                     LADFPYRTAIHWWVFGLAGIAAVVITLLTVSFQAIRAALSNPVESLRAE"
     gene            874..2304
                     /locus_tag="VKQ52_07815"
     CDS             874..2304
                     /locus_tag="VKQ52_07815"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HLZ87129.1"
                     /translation="MPHESISVFDMFKIGVGPSSSHTLGPWRAAQRFTCSLKKSYGLA
                     DLVSVKGLLYGSLAKTGKGHGTDIAIQLGLAGEDPVTFDPGNIDSAIETIRTNKQLVV
                     RGVKPVTFDPAVDIVFLYRETLPYHPNAMTFLAEWSGGQQLAETWYSIGGGFVKKEGD
                     DGASATASTQLPFPIDTNADLLHWCRKTGLAISEIVMENEAAWRPEQETRAGVNKIWT
                     VMRDCTFRGCHAEGMLPGGLNVKRRAADLNRKLIGGNNYHDYDTWIEAIRKGGNSFKY
                     TLDWVSCFALAVNEENASFGRVVTAPTNGAAGVIPAVLQYYVAFCDGYKEEKILQFIL
                     TASEIGSIFKKGATISAAMGGCQAEIGVSSAMAAAALTECVGGSQRQAMMAAEIAMEH
                     HLGLTCDPIGGLVQIPCIERNTMGAIKAITASQLALQSTPDFAKVSLDAVISTMWNTA
                     QDMNSKYKETSDGGLAVQIPLGLSEC"
BASE COUNT          502 a          808 c          584 g          464 t
ORIGIN      
        1 cctccccacc atggggttac agttggtggc cggaagaaac ttccatccgg atatccattc
       61 cgacagtggc aacgtcatca tcaaccagag catggcttca ctcatgggta aagccggcca
      121 ggtaggcgct ttacttacct atggcgatac aaataaaagg tattaccaca tcatcgggat
      181 cgtgaaggat tttctgttca acgaccttgc cggaaccgtc actcccccac tgatgctgga
      241 ctgcggccgg gaggtccagg gagtttataa tttcctcacc atccggctaa aaccgggtaa
      301 ggacctatcc gcccagctgg ccaaagtgga ggccgttatc aaggcaaata accccggcta
      361 tcctgtcgac tatcggtttg tggatgagca attcaacgat atgttcgaat cgcaggccca
      421 catgggagcg ctggccggcg tcttcgccgt gctggccatc ctgatctcct gcctggggct
      481 gtttggcctg gctgcctata ccgccgagcg gcggacaaaa gaaataggga tccgcaaggt
      541 cttgggagct tccgtttccg gtcttgccgc cttgttgtcc atagagttcc tgcagttggt
      601 atcgctctcc tgcctgatcg cctttcccgt ggcctggtgg ctgatgcacg gctggctggc
      661 tgattttccc tatcgtacag ccattcactg gtgggtcttt ggcctggcag ggatcgcggc
      721 cgtcgtgatc acgctgctga cagtaagttt ccaggcgatc cgggctgcac tgtcgaaccc
      781 ggttgagagc ctgagggcgg aatgatttta cacggtagaa ccaccccttc tcacccccaa
      841 aattccgtac ctttgccccc aaatccataa ctcttgccac acgaatccat ctccgtcttc
      901 gacatgttta agatcggcgt cggtccctcc agctcccata ccctaggccc ctggcgcgcc
      961 gcgcaacgct tcacctgctc cctcaaaaaa tcctacggcc tcgccgacct cgtctccgtc
     1021 aagggcctcc tctacggctc cctcgccaaa acaggcaaag gacacggcac cgacatcgcc
     1081 atccagctcg gcctcgccgg tgaagacccc gtcaccttcg accccggcaa catcgattcc
     1141 gccatcgaaa caataagaac gaacaaacaa ctcgtcgtcc gtggcgtgaa gcccgtcacc
     1201 ttcgatcccg ccgtcgatat cgtcttcctc tatcgcgaaa ccttacccta tcaccccaac
     1261 gccatgacct tcctcgccga atggtccggt gggcagcagc ttgccgaaac ctggtactcc
     1321 atcggcggcg gtttcgtaaa aaaggagggc gatgacggag catcggccac cgcctccacc
     1381 caactgccct ttcccatcga cacaaatgcc gatctcctcc actggtgccg caagaccggc
     1441 ctcgccatca gcgagatcgt catggagaac gaagcggcct ggcgccccga acaggagacc
     1501 cgcgccggcg tcaataagat atggaccgtc atgcgcgact gcactttccg cggctgtcac
     1561 gccgaaggaa tgctccccgg cggcctcaac gtcaaacgcc gcgccgccga cctcaaccgc
     1621 aaactcatcg gcgggaacaa ctatcacgac tatgacacct ggatagaggc tatccgcaag
     1681 ggcggtaata gtttcaaata caccctggat tgggtaagct gcttcgccct cgccgtaaac
     1741 gaagagaacg cctccttcgg ccgcgtcgtc accgctccca ccaacggcgc cgccggcgtc
     1801 attcccgccg tactgcagta ctatgtcgcc ttctgcgacg gctataaaga agaaaagatc
     1861 cttcagttca tcctcaccgc ctccgagatc ggcagcatct tcaaaaaagg cgccaccatc
     1921 tccgccgcca tgggcggctg tcaggcggag atcggcgtta gctcggccat ggccgccgca
     1981 gccctcaccg aatgtgtcgg cggctcccag cgccaggcca tgatggctgc cgagatcgcc
     2041 atggaacacc acctcggcct cacctgcgac cccatcggcg gcctcgtcca gatcccctgc
     2101 atcgaacgca acaccatggg cgccataaaa gccatcaccg cctcccagct ggcccttcaa
     2161 agcacccccg acttcgccaa ggtatccctc gacgccgtca taagtacgat gtggaacacc
     2221 gcccaggaca tgaacagcaa atacaaggaa acctccgacg gcggcctcgc cgtgcagatc
     2281 cccctcgggc tgagcgaatg ctagacttca tctttttaca atgcattctc cgcgccccat
     2341 cctaccttcg tccaaaac
//
LOCUS       DATGFG010000421         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_12625, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000421 DATGFG010000000
VERSION     DATGFG010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12625"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VKQ52_07820"
     CDS             <1..665
                     /locus_tag="VKQ52_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HLZ87130.1"
                     /translation="KKSETFSTASDNQPGVQIHVLQGERPMANQNKSLGTFNLDGIPP
                     APRGVPQIEVIFDIDANGILHVTAKDKGTGKEQKIRIEAGSGLNKDEVERMKAEAKAN
                     ESSDKAAREKVDKVNQADSLIFQTEKQLKEFSNKLPADKKAPIEAALNKLKEAHKSQD
                     VAAIDAAVAEMNTAWTAASEEMYKATQQAGGQPGAGAENGGHQSNAGGENVTDAEFEE
                     VK"
     gene            complement(1156..2940)
                     /locus_tag="VKQ52_07825"
     CDS             complement(1156..2940)
                     /locus_tag="VKQ52_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87131.1"
                     /translation="MSTEAFYQQHLDTCNGEIRGIQRRLNGMAVLRLALFVGLGYAIY
                     ALVQDYTLPLLILAVGLAAAFIGCVNLYYRWKDERTLLEKLRFVNTNELGLLAGEHNR
                     FPDGGAFLDSGNYLDDLDIFGRNSLFHLLNRTTTNKGTKALAERLRTPLQDPATIREE
                     QQAVRILAAQTRLRQLLTATGLLIDEKRDDLDGTGLADWLHTPPRLYQKMWLRVVIAL
                     LTAGNLLAVYIFFATNNYTLVVAGIILSRFALGAFSRYIHEQHRLISHKQELLDQYAG
                     ILAVFNKIDTPESPVLQQWQSVTRSAHTAIRRLSRLASYFDQRLNLLVNLLLNTFFLY
                     DLRCMIVLERWKAANRAAFPGWIRAVGAIEQLNSLATFAFNNPGYAYPAPVEDPGTGP
                     FIETTRLAHPLIPATRRVANDFTIGRDERLILVTGSNMSGKTTFLRTIGVNLLMAQCG
                     LPVCAATFTFTPLYLLTSLRISDSLQEQTSYFMAELKKLQKIILRLQTGVPCLVLIDE
                     ILRGTNSEDKTYGSEQFIRKLLQYHCLSLFATHDLSLGALEQPGLITNYCFESVIEND
                     ELHFNYRLQRGIARNRNASFLMKKMDII"
     gene            complement(2954..3106)
                     /locus_tag="VKQ52_07830"
     CDS             complement(2954..3106)
                     /locus_tag="VKQ52_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87132.1"
                     /translation="MAQKKNTAAYWLLFFVSTAAFVWAVASHFPYLTLILPFACTSFV
                     KAMDIM"
     gene            3225..>4229
                     /locus_tag="VKQ52_07835"
     CDS             3225..>4229
                     /locus_tag="VKQ52_07835"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF012889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HLZ87133.1"
                     /translation="MQLKLDFYLRFYTRPGQSILLVGDIPELGGGQLCAAIPLEYVNG
                     EFWHASVALTTEPSSPIYYHYVLRNPDGTLTEEFGDDKFIDPDKMEAPEVQVIDTWNF
                     AGEFENVFFTSPFRNVLLPAHKAVRKARVKGTVTCIFRVKAPLLEENEVVGLLGSATG
                     LNDWKEDDPLLLAPAGDWWTIALPLSHESFPLDYKYGIYNKKEKRLTRFETGPNRHVP
                     GDARPNKLTILHDGFVRLPNSTWHGAGVSIPVFSLRSKDSMGVGEFTDLELLVDWAVK
                     GGLRLIQLLPVHDTTANHNWLDSYPYAAISAFALHPIYLNLEKCAGKKNAALIRPLNK
                     K"
BASE COUNT          989 a         1015 c         1347 g          878 t
ORIGIN      
        1 cgaagaagtc ggagaccttt tctactgcca gcgataacca gccgggtgta cagatccatg
       61 tgttgcaggg tgagcggccg atggcgaatc aaaataagag tctgggaact ttcaacctgg
      121 atggtattcc gccggccccg cgtggcgttc cgcagatcga agtgatcttc gatatcgatg
      181 ccaacggtat cctgcatgta acggccaagg ataagggcac gggcaaggag cagaagatcc
      241 gcatcgaggc cggtagcggt ctgaacaagg atgaagttga acggatgaag gccgaagcca
      301 aggccaacga gagcagcgat aaggcagcgc gggaaaaagt ggacaaggtg aaccaggccg
      361 acagcctgat cttccagacg gaaaagcagt tgaaggaatt cagcaataag ctgcccgccg
      421 acaagaaggc gccgatcgaa gccgccctca ataaactgaa ggaggcgcac aagtcacagg
      481 atgttgccgc catcgacgcc gctgttgcgg agatgaacac tgcgtggacg gccgcttccg
      541 aagaaatgta caaggcaacc cagcaggcag gcgggcaacc cggcgcaggc gccgagaacg
      601 gcggacacca gtcgaatgcc ggtggggaga atgttacgga cgccgagttt gaagaagtga
      661 agtaagtgat tgaacgttag atattttcga aaaggggcct cattttgagg ccctttttct
      721 tgcaggtatc aaaaaaaatc gtacctttgc atcgcaattg aacaaccaaa cgcgcgtcta
      781 gcaagtttgc cagcttcaca agtgctttgt cgaccgacac ttctttcaac gattctcaag
      841 tgttctctac tctcttggtt gttcctatca aattttaaaa acagaacatt tatgaaaagt
      901 gatctagtgc actctattgt gcaaatggaa ccttctatcc tgaaacattg gatgaccgaa
      961 attaaggaaa ctgtggcgac cggtgtcgca atcagcgaag agcgcaagag ctctctatcg
     1021 ttaaacctct gggctatgcg gcggaaaggc cgttatgcgt cgaacagccg caggaagccc
     1081 ggcattatca caggactcgg ttattaaagg atagattatg tagcaactgg cccgcctacc
     1141 ggcgggcctt tttttttata taatatccat ttttttcatc aggaagctgg cattgcggtt
     1201 gcgggcgatg ccgcgttgca gccggtagtt gaaatggagt tcatcgttct cgatcacgct
     1261 ctcgaaacaa tagttggtga tgagtccggg ctgctcgagg gcgccgaggg agaggtcatg
     1321 ggtggcgaag agggagagac agtggtattg gaggagtttg cggatgaact gttcggaacc
     1381 ataggtcttg tcctcggaat tggtgccgcg aaggatctcg tcgatcagga cgaggcaggg
     1441 cacgccggtc tgcagccgga ggatgatctt ctgcagcttt ttcagttcgg ccatgaagta
     1501 ggaggtctgt tcctgcaggg aatcgctgat ccgcagagag gtgagcaggt agaggggggt
     1561 gaaggtgaag gtggcggcgc agacgggcag gccgcactgg gccatcagca ggttgacacc
     1621 gatggtccgc aggaaggtgg tcttccccga catattggaa ccggtgacga ggatgaggcg
     1681 ttcgtcgcgg ccgatggtaa agtcgttagc aacgcgcctg gtggccggga tcaggggatg
     1741 ggccagccgg gtggtttcga tgaagggccc ggtgccgggg tcctctaccg gtgcagggta
     1801 ggcgtagccg gggttgttga aggcaaaggt ggcgagggaa ttgagctgtt cgatggcgcc
     1861 gactgccctg atccaaccgg ggaaggcggc gcggttggcg gccttccagc gttcgaggac
     1921 gatcatacag cgcaggtcgt agagaaaaaa ggtattcagc aggaggttga cgagcaggtt
     1981 cagccgctga tcgaagtagg aggcaaggcg ggagaggcga cgtattgccg tgtgggcgct
     2041 gcgcgtcacc gactgccatt gctgaaggac gggtgactcc ggcgtatcga tcttattgaa
     2101 caccgccagg atgccggcat actgatcgag cagctcctgt ttgtgactga tgagccggtg
     2161 ttgttcgtga atatagcggc tgaaggcgcc gagggcgaac cgggagagga tgatgccggc
     2221 gacgacaagg gtataattgt tggtggcaaa aaaaatgtat accgcaagga gattaccggc
     2281 cgtgagcaag gcgatcacga cgcgcagcca catcttttgg tagagccggg gcggggtatg
     2341 aagccagtcg gccaggccgg taccgtccag gtcgtcccgt ttttcatcga tgagcagtcc
     2401 ggtggcagtg agcagctgac gaagccgggt ctgggccgcc aggatacgaa cggcctgttg
     2461 ttcttcgcgg atcgtggccg ggtcctgcag cggggtgcgc agtctttcgg ccagggcttt
     2521 cgtgcctttg ttcgtggtag tgcggttgag gaggtggaag agggagttcc gtccaaagat
     2581 atccaggtcg tccaggtagt tccctgaatc caggaaggcg ccgccgtcgg ggaagcggtt
     2641 gtgctcgccg gcgagcagcc cgagttcgtt ggtattgacg aagcgcagtt tttccagcag
     2701 ggtgcgctcg tccttccagc ggtagtagag attgacacag ccgataaagg cggccgcgag
     2761 gccgacggca aggatcagta acgggagggt atagtcctgt acaagggcgt agatggcata
     2821 gccgagaccc acgaagagcg caaggcgcag taccgccatg ccattcagtc ttctttggat
     2881 gccccggatc tccccattgc aggtgtcgag gtgttgctga taaaaggcct ctgtagacaa
     2941 gttattgaac agatcacatg atatccatcg ccttgacgaa ggaagtgcag gcaaaaggca
     3001 gaataagcgt cagatagggg aaatgggaag cgacagccca tacgaaggcg gcggtggaga
     3061 cgaaaaacaa taaccagtag gctgcggtat tcttcttttg tgccatgaga tcaaaatttg
     3121 tgcgaaagta agccaaaacc tgtattgaac caaagccaaa aaaatcaata ccttcgggca
     3181 attttggcgt aaattgacag cataatgtaa aaaatacaca ttaaatgcaa ttgaaattgg
     3241 atttctacct gcgcttttat acccgtcccg gccaatccat cctgttggtg ggggatatcc
     3301 ctgaactggg gggtggtcag ctttgcgcgg ctatcccgct cgagtatgtg aacggggagt
     3361 tctggcatgc gagcgtcgcc cttaccacgg aaccgtcttc cccgatatat taccactatg
     3421 tgctgcggaa cccggacggc acgcttacgg aggagttcgg ggatgataag ttcatcgacc
     3481 cggacaagat ggaggcgccg gaggtccagg tgatcgatac ctggaacttt gcaggggagt
     3541 tcgagaatgt cttttttact tccccgttcc gcaatgtcct gttgcccgcg cataaggccg
     3601 tgcggaaggc ccgcgtgaag gggaccgtca cctgtatctt ccgggtaaag gctccattac
     3661 tggaggagaa cgaggtggtg ggtctgctgg gcagtgcgac ggggctgaat gactggaagg
     3721 aggacgaccc gctgctgctg gcgccggccg gtgactggtg gacgatcgcg ttacccttat
     3781 cccatgagtc ctttcccctc gattataagt atgggatcta taataaaaag gagaagcggc
     3841 tgacccgttt tgaaacgggc cccaaccggc atgtgcctgg ggatgcccgg ccgaacaaac
     3901 tgacgatcct ccatgatggc tttgtgcgtc tgcccaattc gacctggcat ggtgcggggg
     3961 tctccattcc cgtattcagc ctgcggagca aggattcgat gggggtggga gagtttaccg
     4021 acctggaatt gctggtggac tgggccgtga agggcgggct ccggctgatc cagcttctcc
     4081 cggtacacga tactacggcc aaccacaact ggctggattc ctatccctat gccgccatct
     4141 cggcttttgc cctgcatccc atttatctca acctggagaa gtgcgcgggc aagaagaacg
     4201 cggcgctgat caggccgctg aacaaaaag
//
LOCUS       DATGFG010000422        13209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1276461, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000422 DATGFG010000000
VERSION     DATGFG010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276461"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VKQ52_07840"
     CDS             <1..423
                     /locus_tag="VKQ52_07840"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HLZ87134.1"
                     /translation="QIVARSYGVDPASGDLPDWPGSFQTITANLNNWSIDNSGLRYVV
                     QAGFSYGAPSPAYLIQELSAQRPILVAYMPSPSSGHAVIITGCSFIPTSSGPLIQSIV
                     VRDPWPSDENIQNDGRVEYPAAMLGSRITACWIVRVFR"
     gene            600..842
                     /locus_tag="VKQ52_07845"
     CDS             600..842
                     /locus_tag="VKQ52_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87135.1"
                     /translation="MSDNKFTVDLGKLKLSEDQLNHINSAIQKAVAGELAQLNLKEQI
                     LLIPVTSSINIQRYPWTRGFIAEVLNKQIIQELVHE"
     gene            853..1284
                     /locus_tag="VKQ52_07850"
     CDS             853..1284
                     /locus_tag="VKQ52_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87136.1"
                     /translation="MPAQEKPDGTAGARSLCPSSRCKPGAELIGVRQDDGTVAILPQP
                     LPVDQDFVDIVSQNALKPEQRFRFSNKCVEGGCSQWTGSRCGVVDQALTMLESVVAGL
                     PECSIRPSCRWFQQSGKDACAVCPYIITHATEKDLIMPLSP"
     gene            complement(1371..1802)
                     /locus_tag="VKQ52_07855"
     CDS             complement(1371..1802)
                     /locus_tag="VKQ52_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87137.1"
                     /translation="MKLFAEAAYISRTGTYTLDLDEANATMGIATSNGVTTSGALNYS
                     TTRYGGGLYFETSRKTDSYIELGAFKETTTLGSNGASDNAYTLTSPMIYRATLVMGKF
                     FLEGSYGPNYPPDGAPLYDLDGNGVAQKSFLEVKIGAVFAL"
     gene            complement(1857..2513)
                     /locus_tag="VKQ52_07860"
     CDS             complement(1857..2513)
                     /locus_tag="VKQ52_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87138.1"
                     /translation="MKRKSSVLPIAFLTGAAGRLLLLAPAVFILLLAGCSKSNDGANN
                     NNNNNNNGPSTTKLIYVNDTYTPIAITVNGVTSSIAVGGSLTYTGTAGTQLSGAAHTS
                     GLTSTGNVVGQVINWTISDQFPSSGSGTKTLDVGSNYFFLKVNNTSMYTITGVYVNFG
                     LVPQTFDNITFGYGLFNIGYYPAYANSNVRCISGGFFWQAYTNLSNAQNQEFLFTLTN
                     "
     gene            complement(2608..3852)
                     /locus_tag="VKQ52_07865"
     CDS             complement(2608..3852)
                     /locus_tag="VKQ52_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ87139.1"
                     /translation="MRKAFLCPKFFVSILFSGAFGYTALGQPRDVAGSPKEPDTSPLK
                     VKGYSTAANFAGGVDKSKVMDFFQDQQFDEALDYLSPVLQADSDNIPVLNYAGYAYYM
                     SDNPSAAQACYRRMLAVDSNSTTALHYLVLLNQNEDATAALGYARRLIGLQPNKAAWW
                     RVAGELFGRKERPDSALVYLGRAYAMAPGDVRTVVALGNLLCDGKAYAKADSIVDIAL
                     QKDSLNVSLLKLRVRSAYWQKQYDEVLAPGEKLLQLKEPSTNSLEWLALSYYDLKQYP
                     DCIRVCERMFDLGLDLEAVYYYEARAQAKMRNYAVSDSLLRKALSKAIGKTAEWYYDD
                     LGDNAEATHRYRQALAHYDTAYYLFKDPLTLYTCGRIAETELHNEALARQYFRRYLAL
                     ARPETAEEKKVYGYVRQRWGTK"
     gene            complement(3939..4202)
                     /locus_tag="VKQ52_07870"
     CDS             complement(3939..4202)
                     /locus_tag="VKQ52_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87140.1"
                     /translation="MKRFLVLAALFAVTVLASGASLPVGPAAHPRVFHKETYVYICNS
                     SGAYAYHSTKSCRGLNRCSHEILKVTLSEAVNTYRRKPCKICE"
     gene            complement(4373..4696)
                     /locus_tag="VKQ52_07875"
     CDS             complement(4373..4696)
                     /locus_tag="VKQ52_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87141.1"
                     /translation="MKIGIKMEITFLFKTYFLIAWGFLWGLVYCAALISDGRKGTVSS
                     IDFIAVALSSISFISGFKLIFMTFTTAVHLNTELEMDKVFTSYGGFCVMWIGVRGVYA
                     RIRRQ"
     gene            complement(4680..4916)
                     /locus_tag="VKQ52_07880"
     CDS             complement(4680..4916)
                     /locus_tag="VKQ52_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87142.1"
                     /translation="MPSNSSEVMEKKESLPVDKIVSLITTGLSGMMGGALLGFDFGVN
                     ATIIGGFIGTVAVCIAEVKSWQKNSTNQKVEDWY"
     gene            complement(4970..5965)
                     /locus_tag="VKQ52_07885"
     CDS             complement(4970..5965)
                     /locus_tag="VKQ52_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87143.1"
                     /translation="MLTIKSLLFIPLCLTVLWCHAQEDSLTRPSTQTSTTQKLNVSKA
                     IAEYAGFYTWDDYVQMYPAGITKDSSYSVGKFPVTSDLYGVFAVQSVDSDQNGVDVNV
                     TIKYLYYMKDKNIRKDSATSTITNYIYFLRYNYSASLEEFNKLTQQEKVSRYFGTAQR
                     YFIIRMSVFKAHAKKVPTKIITKGALGFGILNYPFKLRIQPHINDFTGSFNVGAAVSY
                     TYKHDSLKKWTYSHVLGIGISSITLDKAAVNTDTGAIGSTNSLAALTLSYGFMVQYDK
                     VQLGVFIGWDRISNLNNNTYGWKYQGNPWISIGLGLSIFSTSNNTQLQKNDTQPN"
     gene            6291..6827
                     /locus_tag="VKQ52_07890"
     CDS             6291..6827
                     /locus_tag="VKQ52_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLZ87144.1"
                     /translation="MGKTGLQREMDSFFRETEDDAFSIRRVTKGAFSQSRRKLAPDAF
                     LKLNEIIWRDFYKEVDYLGYHGHKLLAVDGRFLNLPDHASIRKGFGTRGMGRGRLRGK
                     KQSMCLLSMLYDPVNYLTLDVQTDHMDASELQLFLRHLNKVEKDAVVLLDRGYLARYI
                     FFILQSKGSHFIVRMKSN"
     gene            6828..7466
                     /locus_tag="VKQ52_07895"
     CDS             6828..7466
                     /locus_tag="VKQ52_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87145.1"
                     /translation="MQVKEFLKSRQQDMTVMMEVPDGDLDCYRQQFPRMRKQVKCRLV
                     RIKDEQGNIQVLCTSLLDPVKYNLGDLGELYLIRWAIEEGYKMHKARVQVEAFIGKTA
                     IAVKQDIYAKAMMMTLCAALAFPMEERVIKECRADQKKGLVKHPHKINRTYAYWSTKG
                     ILVGMFIKRLVRSALAAFDKQVEANTDAVLPGKRNPRPWKPPRHYYMNYKDV"
     gene            7494..7919
                     /locus_tag="VKQ52_07900"
     CDS             7494..7919
                     /locus_tag="VKQ52_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87146.1"
                     /translation="MKRIIALRGVGNSGKTTTIWQLRDILPARGYPQIPGSFVDHGRD
                     FSESFERNGARVGITSAGDAYDLVHDRVQELIDSHCEIIVCACRSRDNGDHGTNAAIN
                     TFSPPYVIRFIEKIREPDQTLRVVANMNSAEELFRNIEL"
     gene            8158..8925
                     /locus_tag="VKQ52_07905"
     CDS             8158..8925
                     /locus_tag="VKQ52_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87147.1"
                     /translation="MADANLAGFCLLALPNPQIQPLSILTSKDKSHAETTNVDIKNIF
                     VDNTEPLPRPTMDAAILKDASRSVVVELSVEGNANLLQHLLSFLKLSATFKLEKSRSI
                     MVRLENARKTGVNEFDLDAFVNSAKASQKSETFLEMLNKNELFVVTDIIKCKKYAFEY
                     SDNKVTSAQLKVDTPLDGGGNAGVHTTNSAKEDMTYEGDEDITLAVKAYRILYNHDKK
                     TGRDKYRIRKEEALATVKGPEKFSGDRLDAELVLSPQ"
     gene            8933..10447
                     /locus_tag="VKQ52_07910"
     CDS             8933..10447
                     /locus_tag="VKQ52_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HLZ87148.1"
                     /translation="MDEQIISSLVGHLKKGTCILVLGPEFINIDLEDKPFAGSILNYL
                     AECKFKKYKKPTNYLTEDGFFFFDEKNNNDQLIKENILREAAQYYRDLPVTESYETLA
                     QIPFNAIISLSPDKLIDTACRNIGKEPLYRKYTPEEFQDEPTIDSKQSPLIYNLFGDI
                     EDEDVLLFTFDNLFAFLDKLFQNAIFPNFQRRLCKARSFIFLGFTYDKWYLKLIFYLI
                     KKMRMAAQQIESKYAVIDMARDSVDQRIDFFKSHFGMIFSSENEKSFINDLYTACADQ
                     GMLFKDQVAKGEDVDEEEGPPRYRILYLASSPKDLKALDQGPEYFDSIKSKLRGHFYL
                     PMEPVFKVSADTLRKSILGEDGKEPQLVFISCHGGHDNTLLLCGEDGQSAPLSLDDLV
                     EIVSLLATKNRRLECVVFSSCQSAAQAKAISTIIPYAIGMREPILEDASNIFTEGFFE
                     GFLRKRSDLPFAFRNGILKLKACPLDGITYQDLPLLYHKGELVPPNLQTKAIGV"
     gene            10444..>13209
                     /locus_tag="VKQ52_07915"
     CDS             10444..>13209
                     /locus_tag="VKQ52_07915"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ87149.1"
                     /translation="MKKKFRYPGTKSFSTEEEEVFFGREEDIHNLLTSILVNPTTVLF
                     GYSGTGKSSIIRAGLIPQLYRHFVGDDSGFDNYRIIDINPKSHTEGKQLWAEIRRSLI
                     PQLNLEHPFLPEDATIQPTSLWHLLKKDQYNQELADYQLTTLFIVDQAEELFTYSDRD
                     VDEFVSELHPVIGQFVPESFRRRHPERSSEADSDTLVLSRSKTLHAPLRVKFLFSIRS
                     DKLNLITRLKKASTLILQNSFELLALNRDQAFAGIRRPARKTGDFASEKFTITEAAAE
                     KIFSELCTSDGTTFFNPEECRIDPFSLQIICSFIEQQLVIEKGIREVTGSHLGDIKAI
                     INGYYEDAINSLTYTDRPLTDAERDKVRDFLELQMIVDNRRIPIADVIILSNTQYAIT
                     RELLDQLVEKRILKVDNIPGGHMYEITHDSFIRPILEARARRLTTQQAATDKLPQSSV
                     DDLLKDIQDRWASYRKKEARTNLLSTIKKDQPGKKAEITEMMPPPSARSDMELYSIYK
                     KLGEAYIVNKDYSNAIKWLSLAAEIPQANDSTFLVEIYKNRSNAYFYQKNFEASTEDL
                     KKVLRLLPGDRQAIGYLIENYDLQDKFSEGIAWLDKEQVITSENIGVVTNMGFKLIDA
                     GNYEKATQYLQATLSRLPADYDASRGMGMVESFLKHPEPAKAWFENAFAADPQNPEPY
                     SDIGYMYYINGDLDNAIEWYQKSLVIKPDFADSIVGLSRAYYDKGDKEGNREYLKLAH
                     EAYLKLSSGTITIENYHYNLGLLEEALGDLDQAAENYSKAIVSEPAFEDAHTRLTDLL
                     LLQKQAGMLEQLYRGLMTQKPESAKFYQVYLGLAFEKQRRSEEAIELYRQAIAIDPAY
                     VHPHYRLGDIYRYQDQPEAAIEEYRKAMELEPANSYFPCVLGYVYRYLGNYDESIFCF
                     NQAIKLSP"
BASE COUNT         3504 a         3678 c         3066 g         2961 t
ORIGIN      
        1 cagatcgtcg ccagaagcta tggtgtggac ccagcctccg gtgacctccc ggattggcca
       61 ggcagtttcc agacgattac ggccaatctg aacaattgga gcatcgataa ttcaggatta
      121 cggtacgttg tgcaggccgg cttttcctat ggtgcaccca gcccggcgta cctgatccag
      181 gaattgtccg cgcagcggcc gatattggtc gcgtatatgc cttccccatc atccggccat
      241 gctgtgatca tcacgggctg cagctttata ccaacgagca gcggcccctt gatccagtcc
      301 atcgttgtca gggatccctg gccaagcgat gaaaatattc aaaatgacgg tagggtagaa
      361 tatcctgcgg cgatgctggg tagccgcatc actgcctgct ggatcgtacg ggtatttcgt
      421 tagctggtca cctacggtga aatgggtggc catccgccct tcacgtattt ttacgcttgt
      481 tttcttcccc gcattccctt accttcatat catccccctg aacattccca cctgtcgttc
      541 atccgcccca cccgggtagc cgaaaacggg atcaagagta ggcacaacat tttcttgcca
      601 tgagtgataa caaattcacc gtcgacctcg gtaagctcaa gctctccgaa gaccagctca
      661 accacatcaa cagcgctatt cagaaagccg tcgccggcga actcgcgcaa ctcaatttaa
      721 aggaacagat cctgttgatc cccgtaacct cgtcgatcaa catccaacgt tatccctgga
      781 cacggggctt catcgccgaa gtccttaaca aacagatcat ccaggagctc gtccacgaat
      841 aaagcccggc ctatgccagc acaggaaaaa cctgacggta ccgcaggtgc aagatcacta
      901 tgcccgtcca gtcgttgcaa accgggtgcg gagcttatcg gagtccggca ggacgacggc
      961 acggtagcca tcctgccgca acccttgccc gtcgatcagg actttgtcga catcgtgtca
     1021 caaaacgccc tgaagcccga gcaacgtttt cgttttagca ataaatgcgt ggaaggcggt
     1081 tgttcacaat ggacaggttc ccgctgcggc gtcgtagatc aggccctcac gatgctggag
     1141 agtgtcgtgg ccggcctgcc cgaatgcagc atccggccct cctgtcgctg gtttcaacaa
     1201 agcggcaagg atgcctgcgc cgtctgtccc tatatcatca cgcatgcgac ggagaaggat
     1261 ctgatcatgc cgcttagccc ctgagcctcg cgctgttcat ttcctccccc atcgctctct
     1321 tacctatcca aactccgtat caaaatgcgc cgccgccgtt ccggcgatcc ctacagggca
     1381 aagacagccc ctatcttgac ctccaaaaaa gacttttgag ccacgccgtt gccatccagg
     1441 tcatacaagg gagctccgtc cgggggatag ttgggaccgt aactgccttc caggaaaaat
     1501 ttgcccataa ccaaggtcgc cctgtagatc atcggggaag tcagtgtata ggcattgtcc
     1561 gaggctccat tactgcccag ggtggtagtt tccttaaagg cgcccaactc gatatagcta
     1621 tcggtcttcc ggctcgtttc aaaatagagg ccgccgccgt atcttgttgt ggaataattt
     1681 aaagcgccgc tggtagtcac gccattgctc gtggcaatac ccattgtggc attcgcctca
     1741 tcaagatcca atgtgtaggt cccggtacgg cttatgtagg ctgcttcggc aaacagcttc
     1801 acggttgtcc ctaataaaag cttggcatcg ccggtaaaat caaaataata accgggctaa
     1861 ttggtcagcg tgaataggaa ttcctggttc tgggcattgc tgagattggt atatgcctgc
     1921 cagaaaaacc caccgcttat gcagcgaaca ttggaattcg cataagccgg gtaatatccg
     1981 atgttgaata atccatagcc gaaagtgata ttatcgaatg tctggggaac cagcccgaag
     2041 tttacgtata ctccggtaat ggtatacatc gaagtattat tcactttcag gaaaaagtaa
     2101 ttcgatccga cgtcgagtgt ttttgtgccc gaacccgaag aagggaattg atcgcttatt
     2161 gtccaattga taacctgccc cacaacattt cctgttgagg tgagccctga ggtatgggca
     2221 gcaccggaaa gctgtgtccc ggctgtcccg gtataggtga gggagccgcc aaccgctatc
     2281 gaagaggtaa cgccattgac ggtaattgca atgggtgtat aagtgtcgtt tacatatata
     2341 agcttggtag tgctgggccc attattattg ttgttgttat tgttattggc gccgtcgttg
     2401 gattttgagc atccggcaag cagcaggata aacactgccg gcgccagcag cagtaggcgt
     2461 ccggccgcac ccgttaaaaa agcgattgga agaacagatg atttacgttt cataacatgt
     2521 gttttttaat gtaccaaaac aacacattta tgaaaccttg ggaaacgggg tttttctcgt
     2581 tcttaaaggg gtgttatcac ctgctgatca ttttgtcccc caccgttgcc gtacataccc
     2641 gtacaccttc ttctcctccg ccgtctccgg ccgggcgaga gcaaggtagc gccggaagta
     2701 ttgccgggcg agtgcctcat tatgcaactc cgtctccgcg atgcgcccac aggtatacag
     2761 cgtaaggggg tctttgaata agtaatacgc cgtatcgtaa tgcgcaagtg cctgccggta
     2821 tcgatgggtg gcttctgcat tatccccaag atcatcataa taccattccg ccgtcttccc
     2881 gatcgccttg ctcaacgcct tccgcaacag cgagtcgctc accgcatagt tccgcatctt
     2941 cgcctgggcc cgcgcctcat aataatacac cgcctcgaga tccaggccca gatcgaacat
     3001 tctctcgcat acccgtatac agtcgggata ctgtttcaga tcataatagg acaacgccag
     3061 ccattccagg gaattggtcg acggctcctt cagttgcaac agcttctccc cgggcgccag
     3121 cacttcgtca tactgcttct gccagtaggc gctccgcacc cgcaacttca acagcgagac
     3181 attcagcgaa tccttctgca acgcaatatc gacgatgctg tccgcctttg cataggcctt
     3241 cccgtcacaa agcaggttgc caagggctac cacggtccgc acatcccccg gcgccatcgc
     3301 ataagcccgg cccagataga ccagcgccga gtccggtcgc tccttccggc caaacaactc
     3361 gccggcgaca cgccaccacg ccgccttatt gggttggagc ccgatcagcc gccgcgcgta
     3421 tcccaacgcc gccgtggcat cctcattctg gttcaataac accaggtaat gcagggccgt
     3481 ggtcgaattg gaatccaccg ccaacatccg gcggtaacag gcctgcgccg cggaggggtt
     3541 atcactcata taataagcat aaccggcata gttgagaacg ggaatattat ccgagtccgc
     3601 ctgtaacacc ggggagagat agtccagcgc ctcgtcgaat tgctgatcct ggaaaaagtc
     3661 catcaccttg ctcttatcga ccccgccggc aaagttcgcc gcggtggaat agccctttac
     3721 tttcaacggg gaggtgtccg gctccttcgg gcttcccgcg acatcccgcg gctgccccaa
     3781 cgcagtatac ccaaacgccc cggaaaataa gatagaaacg aaaaatttag ggcacaaaaa
     3841 agcctttctc atagtatcta tggtttgcct gttgatgcag tccggcaaaa tttccataaa
     3901 cccaacgcaa aaaaaccccg ccccaaggcc tcgcggccct attcgcagat cttacagggc
     3961 ttccgccggt aggtgttcac cgcttccgac agcgtgacct tcaggatctc atgggaacag
     4021 cggttcaatc cacggcagga cttggtgctg tgataggcat acgccccact gctgttacag
     4081 atatagacat aggtctcctt gtggaagacc cgggggtggg cagcagggcc cacaggcagg
     4141 gaagctcccg acgccaaaac cgtgaccgcg aaaagcgcgg cgaggaccag gaatcttttc
     4201 atagcaggca atctttgcca cgcaaattac accacagcca acacccggtc aaccgtgaaa
     4261 acactaatgt cgtttgctca agcgaaaaag gagtgtttta acgaacaaca acaatagttt
     4321 tcttcatagg acatatcagc cagcccacta ttgcaaaaca aaatgttatg cgttactgtc
     4381 ttcttattct cgcgtataca cctctaactc ctatccacat tacgcaaaac ccgccataag
     4441 aagtaaaaac tttgtccatt tccaattctg tatttaagtg cactgcagtt gtaaaagtca
     4501 taaaaattaa tttgaaacca gaaataaaag aaatcgaaga aagcgctact gcaataaaat
     4561 caatggaact cactgtgcct ttacggccgt ctgatattag tgcggcacaa tagaccagtc
     4621 cccacaggaa tccccaggca atcaaaaaat aagtcttgaa caagaaggtt atttccattt
     4681 taataccaat cttcaacttt ctggttagtt gaatttttct gccaactttt aacctccgca
     4741 atgcatactg ccacagtgcc tataaaacca ccaataatgg tagcgttaac cccaaagtcg
     4801 aatcccagaa gtgctcctcc catcattcct gaaagtcctg tcgttattaa cgaaactatt
     4861 ttgtccacag gaagactttc ttttttctcc attacctcac tcgaattact aggcatattc
     4921 aaattatttg tgctgtcctc ggtataatcc agactgccgc ttatcactgc taattgggct
     4981 gcgtatcgtt tttttgtagc tgggtattat tacttgtcga aaaaatggaa agtcctaagc
     5041 ctatacttat ccagggattt ccctggtact tccagccata ggtattgtta tttagattgc
     5101 tgatccgatc ccaccctatg aaaacaccta gttgaacctt atcgtactga accatgaacc
     5161 cataactgag cgtcaatgcg gccagggaat tggtggagcc gatcgcaccg gtgtcggtat
     5221 ttacggcagc cttatccagg gtaatactgg agatgcctat tcccaataca tgggaatagg
     5281 tccatttttt aagggaatca tgcttgtagg tatatgatac ggcggcgccg acattaaaag
     5341 atcctgtgaa atcattaata tgcggctgaa ttcgaagttt gaaagggtaa ttaagtattc
     5401 cgaagcccaa agcgcccttc gtaatgattt ttgtaggtac ttttttggcg tgtgctttga
     5461 agacactcat tcttataatg aaatatcttt gagcggtgcc aaaatatctt gacaccttct
     5521 cttgctgtgt taacttattg aattcctcca gcgaagccga ataattatat cgtaagaaat
     5581 atatatagtt ggttattgta gaagtggcag agtcctttct tatattttta tccttcatgt
     5641 agtagaggta ttttatcgtc acattcacat cgactccatt ttgatctgaa tcaacgcttt
     5701 gaaccgcaaa tactccataa agatcactgg taactgggaa tttgccgaca gaataagaac
     5761 tatcttttgt tattccagct ggatacatct gaacgtaatc atcccaagta tagaaaccgg
     5821 cgtactcagc tatggcttta cttacattaa gtttttgtgt ggtggaggtt tgagtggaag
     5881 gccttgtcaa gctgtcttct tgcgcgtgac accataagac tgtaagacag agcggaataa
     5941 aaagtaagct cttaattgtc agcatcgtag ttaattttgg tatcgcaaaa tacgatttgc
     6001 tatatgctca tcccaccgtt aaaacaccca tatcgtttag ttaggcaaaa aaaggtgatt
     6061 tcacccccca gcaattggag caggtgccgt aaatttattt ggtatataag aagggggtaa
     6121 tctttcatcg gaaaaccaat caagactatc ggatcagagt atcgaaaaaa ttaatctacc
     6181 atatcaatga tgacactgaa ttcaaaggcc gtcacacgaa cggccaggaa ggggcctgca
     6241 ccagaaatcg gctgcttcca ttcagttatc tgataatctg cttacttagg atgggaaaga
     6301 cgggactaca gcgggagatg gatagcttct ttcgggaaac ggaggacgat gcgttcagca
     6361 tacgacgagt aacaaaaggg gccttcagtc agtcgcgcag aaagctggcg cctgacgcct
     6421 tcctgaaact caatgagatc atctggcggg atttttataa agaagtggac tacctgggtt
     6481 atcatggtca taaactgtta gcagtggatg gccgtttcct gaatcttccg gatcatgcca
     6541 gcattcggaa aggatttggt accaggggaa tgggccgggg aaggctaaga ggaaaaaagc
     6601 agagcatgtg cctgttgtcg atgctctatg atccagtgaa ttacctgacc cttgacgtgc
     6661 agactgatca tatggatgcc agtgaactgc agttgttctt acggcacctg aacaaggtag
     6721 aaaaggatgc tgtcgttttg ctggaccggg gttaccttgc ccgttatatc ttcttcatcc
     6781 tccaatcgaa gggaagtcat tttatcgtcc ggatgaaatc gaactagttg caggtaaaag
     6841 aattcttaaa aagccgccag caggacatga ctgtcatgat ggaggtaccg gacggtgatc
     6901 ttgattgcta ccggcaacag tttccacgga tgagaaagca agtgaaatgc aggctggtaa
     6961 ggatcaagga cgagcaaggg aacatacagg tgttgtgtac ttcactgctg gatcctgtca
     7021 aatacaacct gggggattta ggcgaactct atctgattcg ctgggcaata gaagaaggat
     7081 acaaaatgca caaggcgcgg gtgcaggtgg aagccttcat cggcaagacg gcaatcgcag
     7141 tgaaacagga tatttacgcc aaagctatga tgatgacctt atgcgctgct ttggcattcc
     7201 caatggagga acgggtgata aaagaatgtc gggctgacca aaagaaagga ttggtaaagc
     7261 atcctcataa gatcaaccgt acttatgcct attggtctac caaaggtatc ctggtaggaa
     7321 tgtttattaa aaggcttgtc cgatcagcgc tggctgcctt tgacaagcag gtggaagcca
     7381 ataccgacgc ggtcctcccg ggaaaaagaa acccgagacc ctggaaacct ccaagacatt
     7441 attatatgaa ctacaaagac gtttgatcct gttttaattt aaccaactta tttatgaaaa
     7501 gaatcatcgc tctaagaggg gttggcaata gcggaaaaac gacaacaatt tggcaactac
     7561 gagatatttt gccagcgaga ggctatccgc agataccagg ttcttttgtg gatcacggaa
     7621 gagatttttc agaaagcttt gaaagaaacg gagcaagagt gggaattaca agtgcaggag
     7681 acgcttacga cctggtacac gatcgcgttc aagaactaat tgatagccat tgtgaaatta
     7741 tagtttgtgc ttgcagaagt cgcgataatg gcgaccatgg cacaaatgca gcaataaata
     7801 ctttttctcc tccttatgta atccggttta ttgaaaagat ccgagagccg gaccaaacac
     7861 taagagttgt ggctaatatg aactccgctg aggaattgtt cagaaacatt gaattataga
     7921 cttatagcat gctctcaaag atttacttcg agggctcaaa aagtatacgc aagattgact
     7981 agcccccttc tgaatttggg tgagaaacat ctgggcttgg aagcttaact aaacagcatg
     8041 ggttaagaca cccatttctg ccttaagtca acgatattag tgaaaacacc cataaaccgt
     8101 ataaataccc ttggtaaatt ccaatttata cattagcttt cttttaaatc ctaatttatg
     8161 gccgacgcaa acctcgccgg attctgcctg ttggcgctcc ccaacccgca gatccagccc
     8221 ctcagcattc tcacctcaaa ggataagtcg cacgccgaaa ccaccaacgt ggatatcaaa
     8281 aacatcttcg tcgacaacac cgaacccctg cctcgaccta ccatggacgc cgccatcctc
     8341 aaagacgcca gccgcagcgt cgtcgtcgaa ctgtccgtcg aaggaaatgc caacctgcta
     8401 cagcacctcc tctccttcct caaactatcc gcaaccttca agctggaaaa aagccgctcc
     8461 atcatggtcc gcctcgagaa cgcccggaag acaggcgtca acgagtttga cctcgacgcc
     8521 ttcgtcaaca gcgccaaagc cagccagaaa agcgagacct tcctcgaaat gctcaacaag
     8581 aacgagctct tcgtcgtcac cgatatcatc aaatgcaaaa aatacgcctt tgaatacagc
     8641 gacaacaaag tcaccagcgc ccaactgaaa gtcgacacac ccctcgacgg cggcggcaat
     8701 gccggcgtgc acaccaccaa cagcgcaaaa gaagacatga cctacgaagg agacgaagac
     8761 atcaccctcg ccgtcaaagc ctatcgcatc ctttacaacc acgacaagaa aaccggccgc
     8821 gacaagtatc gcatccgcaa agaagaagca ctggccaccg tcaaaggacc cgaaaagttc
     8881 tccggcgatc gcctggacgc cgaactagtc ctatcacctc aataacccga ccatggacga
     8941 gcaaatcata tcaagcctcg tgggccatct gaaaaaagga acatgcatcc ttgtgctggg
     9001 ccccgaattc atcaacatcg accttgagga caaaccattc gccggcagca tcctcaacta
     9061 tctcgccgaa tgcaagttca agaaatacaa aaaacccacc aactatctca cggaggacgg
     9121 gttcttcttc ttcgacgaga agaacaacaa cgaccagctc atcaaggaga acatcctcag
     9181 ggaggccgcc cagtattacc gcgacctgcc cgtaacggag tcctatgaga ccctggcaca
     9241 aattccgttc aacgccatca tttccctgtc ccccgacaag ctcatcgata cagcctgccg
     9301 caacatcggg aaagagccgc tttaccgcaa gtataccccc gaagaattcc aggacgagcc
     9361 taccatcgat tccaaacagt cgcccctcat ctacaatctc ttcggcgaca tcgaggacga
     9421 agacgtgctc ctgttcacct tcgacaatct cttcgccttt ctcgacaagc tgttccagaa
     9481 cgccatcttc cccaacttcc agcgccggct ctgcaaggcc cgcagcttta tcttccttgg
     9541 cttcacctat gataaatggt atctcaaact catcttctat ctcatcaaga aaatgcgcat
     9601 ggcagcgcag cagatcgagt ccaaatacgc cgtcatcgat atggcccggg attcggttga
     9661 ccagcggatc gatttcttca aaagccactt cgggatgatc ttttcttccg agaacgaaaa
     9721 gtcctttatc aacgacctct acaccgcctg cgccgaccag ggcatgctgt tcaaagacca
     9781 ggtggcaaaa ggcgaagacg tggacgaaga ggaaggcccg ccccgctatc gcatcctgta
     9841 cctggcttcc tcgccaaaag acctcaaggc cctcgatcag ggacccgaat atttcgattc
     9901 cataaaaagt aagttgcggg gacatttcta cctgcccatg gaacccgtct tcaaggtcag
     9961 cgccgacacc ctgcgcaaat cgatcctcgg agaagatggt aaggaacccc aactggtgtt
    10021 catcagttgt cacggcggtc acgataatac cctcctgcta tgcggcgagg acgggcaatc
    10081 cgcccccctg tcactggacg atcttgttga gatcgttagt ttgctggcca ccaagaaccg
    10141 ccggctggag tgcgtcgtct tctccagctg tcagtcggcg gcgcaggcaa aagccatctc
    10201 caccatcatc ccctacgcca tcggcatgcg cgagcccatc ctcgaggacg ccagcaatat
    10261 cttcaccgaa gggttcttcg aaggattcct gagaaaaaga agcgatctgc ccttcgcctt
    10321 ccgcaacggc atcctcaaat taaaggcctg tcccctggac ggcatcacct accaggatct
    10381 tcccctgctc taccacaaag gcgaactcgt tccacccaat cttcagacaa aagccatcgg
    10441 ggtatgaaaa agaaattcag gtatccaggt acgaaatcct tctccacgga agaagaagag
    10501 gtcttcttcg gcagggaaga ggacatccac aacctcctca cctccatcct cgtcaatccc
    10561 accacggtgc tcttcggtta ctcgggcacg gggaaatcct cgatcatccg ggcggggctg
    10621 atcccccagt tgtaccggca ttttgtcggc gatgacagcg gcttcgacaa ctaccgcatt
    10681 atcgatatca accccaaaag ccatacggaa gggaaacaat tgtgggcgga gatccgcaga
    10741 agcctcatcc cccagttgaa cctggagcat cccttcctgc cggaagacgc taccatccag
    10801 ccgacctccc tctggcatct gttgaaaaaa gaccagtaca accaggaatt ggccgactac
    10861 cagctaacca ccctgttcat tgtcgaccag gcggaagagc tgttcaccta ttccgaccgc
    10921 gacgtcgatg aattcgtctc cgagctgcat cccgtcatcg gccagtttgt cccggaatcc
    10981 ttccggcgcc ggcatcccga aagatccagc gaagccgaca gcgataccct cgtcctttcc
    11041 cgcagcaaga ccctgcatgc gcccctgcgg gtcaaattcc ttttctccat ccgcagcgac
    11101 aagctgaacc tgatcacccg gctgaaaaaa gcaagcaccc tcattctaca gaacagcttc
    11161 gagctgctcg cgctcaaccg ggatcaggcc tttgccggca tccgccgccc cgcaagaaaa
    11221 acgggggact tcgccagcga aaaattcacg atcaccgaag ccgcggcgga gaagatcttc
    11281 agcgaactct gcacctccga cggcactacg ttcttcaatc ccgaagaatg ccgcatcgac
    11341 cccttcagcc tccagatcat ctgcagcttc atcgagcagc agctggtcat cgaaaaaggc
    11401 atccgcgaag tgaccgggag ccacctgggg gatatcaagg ccatcatcaa tggctattac
    11461 gaggatgcca ttaacagcct cacctacacc gaccggccac tcaccgatgc ggaaagggat
    11521 aaggtccgcg acttcctcga attgcagatg atcgtcgaca accggcgcat acccatcgcc
    11581 gatgtcatca tcttaagcaa tactcagtat gccatcacgc gggaattgct ggaccaattg
    11641 gtggaaaagc gcattctcaa agtggacaat attcccggcg ggcatatgta cgagatcacg
    11701 cacgacagct tcatccgccc catactcgag gcccgcgccc ggcggctgac cacccagcag
    11761 gccgccaccg ataagttacc ccaatccagc gtcgacgatc tgctaaagga tatccaggac
    11821 agatgggcga gctaccggaa aaaagaagcc cggaccaacc tgctgtccac catcaagaaa
    11881 gaccagccgg gtaagaaggc ggaaataacg gaaatgatgc ctcctccctc ggcccggtcg
    11941 gacatggagc tgtacagtat ttacaaaaaa ctgggcgaag cctatattgt caacaaggac
    12001 tattcgaatg ccatcaaatg gcttagcctg gccgccgaga tcccgcaggc caacgacagc
    12061 accttcctcg tggagatcta taaaaaccgg tcgaacgcct atttctatca gaagaatttc
    12121 gaagcctcca cggaggatct caagaaggtg ctccggctgt tgccgggcga tcgccaggcg
    12181 ataggctatc tcatcgagaa ttacgatctc caggacaaat tcagcgaagg catcgcctgg
    12241 ctcgacaagg agcaggtcat caccagcgag aatatcggcg tggtcaccaa catgggattt
    12301 aaactcatcg acgccggcaa ttacgaaaag gctacgcagt atttacaggc gacgctcagc
    12361 cgcctgcctg ccgactacga tgccagccgg ggaatgggga tggtggaaag ctttctcaaa
    12421 cacccggaac ccgcaaaggc ctggttcgaa aatgccttcg ctgccgaccc gcaaaacccg
    12481 gaaccctaca gcgatatcgg gtatatgtat tatatcaacg gcgacctcga caacgccatc
    12541 gaatggtatc agaagtccct ggtcataaaa cccgatttcg ccgactccat cgtgggcctg
    12601 tcccgggcct attacgacaa aggcgacaag gagggcaacc gggagtatct taagctcgcg
    12661 cacgaggcct atctaaaact ttcctccggc actatcacca tcgagaatta tcactataat
    12721 ctcggcctcc tcgaagaagc cctcggcgat ctggaccagg ctgccgagaa ctacagcaag
    12781 gccatcgtca gcgaaccggc cttcgaagac gcgcacaccc gcctcaccga tctcctcctg
    12841 ctgcaaaaac aggccggaat gctggagcag ttgtaccgcg gcctgatgac tcaaaaaccg
    12901 gaatcagcca aattctacca ggtctacctg ggcctggcat tcgaaaagca gcgccgctcc
    12961 gaagaggcga tcgagctata tcgccaggcg atcgccatcg atcctgccta tgttcacccc
    13021 cactaccggc tgggcgatat ctatcgctac caggaccaac ccgaagccgc catcgaggaa
    13081 taccggaagg cgatggaact ggagcccgcg aacagctatt tcccctgtgt gctgggctat
    13141 gtctatcgct atctcggcaa ttacgacgaa tccatcttct gcttcaacca ggccatcaaa
    13201 ctctcaccg
//
LOCUS       DATGFG010000423         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_833050, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000423 DATGFG010000000
VERSION     DATGFG010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833050"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VKQ52_07920"
     CDS             <1..283
                     /locus_tag="VKQ52_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HLZ87150.1"
                     /translation="ELKRMADSVNAILPQQPEAGDEDLSLERIPGMGFDTAYLREEIR
                     DQDSAMTLYRNEVANGSYVGLIYYANKYLPLIQSRRMEADSLLQLMEHP"
     gene            439..2979
                     /locus_tag="VKQ52_07925"
     CDS             439..2979
                     /locus_tag="VKQ52_07925"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLZ87151.1"
                     /translation="MKHPSLIPTERFLIAAFILCIGGCLLIYQQIDRQHAKADLVTHS
                     YIVKITLNQLLLNLTEAETNQRGYLLTHDSDFLQHYDDAMHKSRSLINILDSLTVDNS
                     HQREQLDKLRNQFNSEVVNLYTSMILPDGKDVTEEGSEAIAGNTREILNSLRITVYSM
                     LAAEDRLLQQRILDKEASTKVNPYLSMIVFLLFLLLSFLVYLKLKKETDLRLDSDEQL
                     ATQNAIFHQAEKMALIGSYTWNAATGEMRLSDNLCRLLGYEPQESALSPGQLLRLVHP
                     ADKHRVTYNGKPTLERLMRAEMPCRFITTDGIIKYLHVTGSPVYKPEGEVIVGTFQDV
                     TREIELRQTLENNDNFIRSLLGVAPSIITIFDVESRSIVFTSRRYFHSAGRSPELPSG
                     LADLAGMIHPEDRKAALHYFQQLQRLPDMEMAEFEYRVRGIEGDYHYWLTRSTPYKRN
                     GDNMAIQFISATIDITEKVRAEMELKAKSEALLISRYEQQSASRFKTMADSMPQIVWT
                     AKPTGEIDYFNQRWYEIMQGGAADPTTSEAPDLVENMHPDDRTAYFSHWDSCLSTGRP
                     FQMEFRFSDHKHPGEYCWFLAKALPIYDQGGAVLRWHGTFTDINDQKLAQLAIQQLVK
                     EKEDFISIASHELKTPVTILKGSIQLLEQEAGTITLPAETSQLIEMAGRQVNKLTRMV
                     ADLLDVAKLNGERVRLAKSDLLLGEVIEESIEQVSGPGIRDSIRVTGTTDVRVHANRE
                     RIGQVITNFLSNAKKYSPEGSVIAIDVSQEDHCVRVAVRDEGIGIPKSKLPFVFDRFF
                     RVEESAGRISGLGLGLYISAEIVALHQGEIGVDSEPGKGSTFWFTLPV"
     gene            3433..3738
                     /locus_tag="VKQ52_07930"
     CDS             3433..3738
                     /locus_tag="VKQ52_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87152.1"
                     /translation="MKGNVTHTIFFIYDVRDHALKFSLKAEVVCNDAGVYTVSNIRPE
                     SSDNDGVLLPPIQLKKENGGWIFLDNGQESILSNTIGRAIEDSLIGENHTHRHSPKQ"
BASE COUNT          899 a          917 c         1084 g          863 t
ORIGIN      
        1 cgaactcaag cggatggcgg atagcgtcaa tgccatcctt cctcaacaac ccgaagcggg
       61 agacgaagac ctttcgctgg aaaggatccc gggaatgggt ttcgacaccg cctatctccg
      121 cgaagagatc cgggaccagg acagcgccat gaccctttac cggaatgagg tcgcgaacgg
      181 gagttatgtc ggtcttatat actatgcaaa taaatatctc ccgctcattc aatcgagaag
      241 aatggaagcc gacagtttgt tacaattgat ggaacaccct taagagtttt ccccaatcca
      301 gggggttggt ccggcatttg cgtattatgc aataggcttt taataggata ttggatttta
      361 gcaacggcct ggatttctat ccgggctttt ttgtgtagaa acccgaactt tggagaaccg
      421 atctcaacct attgtcgcat gaagcatccc agtctgatcc cgacggaacg ctttctcatt
      481 gccgccttta tactttgtat cggcggctgc ctgttaattt atcaacagat agacaggcag
      541 catgcgaagg ccgaccttgt aacgcatagc tacattgtta agatcacgct taatcaattg
      601 ttgctcaatc tcaccgaagc cgagaccaat caacggggct acctgctcac gcatgattca
      661 gatttcctcc aacattatga tgatgccatg cataaatcga ggagtctcat caacattctc
      721 gattcgctga ctgtggacaa ttctcatcag cgggaacaac tggacaaatt gcggaatcaa
      781 ttcaacagtg aggtggtgaa tctctacacc agtatgatcc tgccggacgg aaaggatgtg
      841 accgaagaag gcagtgaggc tattgcagga aataccaggg agatattgaa tagtctacgg
      901 atcaccgttt actcgatgct ggcggctgag gaccggttgc ttcagcagcg gatactggac
      961 aaagaagcct cgacgaaggt gaacccctac ttgtcgatga tcgtcttcct tctttttctg
     1021 ctgttgagtt tcctggtata cctgaagttg aagaaggaga ccgatctgcg gctcgattcg
     1081 gatgaacaat tagcgacaca gaacgccatt tttcaccagg ccgagaagat ggccctgatc
     1141 gggagctaca cctggaatgc cgccaccggc gagatgcggc tctcggataa cttatgccgg
     1201 ctgctgggct acgagccgca ggagtcggcg ctttcccccg ggcagctgct gcggctggtg
     1261 catccggccg acaaacaccg cgtcacctat aatgggaagc ctacgctgga gaggctgatg
     1321 cgggcggaga tgccctgccg gttcatcacc accgacggga tcatcaaata cctgcatgtc
     1381 accggcagtc ccgtatacaa acccgagggg gaggtgatcg tcggcacgtt ccaggatgtc
     1441 accagggaga tagagttacg acagacactc gagaacaatg ataatttcat ccggagcctg
     1501 cttggggtgg cgcccagcat catcaccatc ttcgacgtgg aaagccggag cattgtgttt
     1561 accagccgac ggtattttca ttcggcgggc cggtcgcccg agctgccctc cggcctggcc
     1621 gatctggccg ggatgatcca tccggaagac cggaaggccg ccctccacta tttccagcaa
     1681 ttgcagcggc tgccggatat ggaaatggcg gagttcgagt accgggtacg ggggatcgag
     1741 ggggactatc attattggct tacccgcagc acaccttata agcggaacgg cgataatatg
     1801 gccatacagt tcatcagcgc caccatcgat atcacggaaa aggtgcgggc cgagatggag
     1861 ctgaaggcca agagcgaggc gctgctcatc tcgcgctacg agcagcaaag cgcatcccgg
     1921 ttcaagacca tggccgactc gatgccgcag atagtctgga cggcaaagcc aacgggagag
     1981 atcgattatt tcaaccagcg atggtacgag atcatgcagg gtggcgccgc cgaccccacc
     2041 acctccgagg ccccggacct ggtggagaac atgcatcccg acgaccgcac cgcttatttt
     2101 tcccattggg attcctgtct ttccacgggg aggcctttcc agatggagtt ccggttcagt
     2161 gatcacaaac atcccgggga atattgctgg ttcctggcga aggccttacc gatctatgac
     2221 cagggcggag cggtgttgag atggcatggg acgttcaccg atatcaacga tcaaaagctt
     2281 gcgcaattag ctatccagca actcgtgaag gaaaaggaag actttatcag catcgccagt
     2341 catgaattga aaacccccgt gacgatctta aaggggtcga tccaactgct cgagcaggag
     2401 gccgggacca ttacgctgcc cgcggagacg tcgcagttga tagagatggc ggggagacag
     2461 gtgaataagc ttacccggat ggtcgccgat ctgctggatg tcgccaaatt gaacggggag
     2521 cgggtccgcc tggccaagag cgatcttctt ttgggcgagg tgatcgagga gagtatcgag
     2581 caggtgagcg gaccgggaat acgagattcg atccgggtca cgggtaccac ggatgtgcgg
     2641 gttcatgcca accgggagcg gatcggccag gtgatcacga attttttatc gaacgcgaaa
     2701 aagtactctc ccgaggggtc tgttattgcg atcgatgtgt cacaggagga ccattgtgtc
     2761 cgggtggccg tcagggatga ggggatcggc attccgaagt cgaagctgcc ctttgtcttt
     2821 gaccggttct tccgggtgga ggaatccgcg gggaggatct ccgggttggg gctggggctg
     2881 tatatctcgg cggagattgt cgcccttcac cagggggaga tcggggtgga cagcgagccg
     2941 ggaaaggggt cgacgttttg gtttacgctg ccggtctgag ggtggggatt gcggtttgaa
     3001 gtgagggatg gcggttgggg tgagggtttg cgaatggggc gatcgcggga agttgggaaa
     3061 caaaaagacc atccttaccc gtgtaagtga tggtcttgac ccttaaaaca acctgtcgca
     3121 agagttaaaa tactttttag cctttatata gaattagcac tacaaccatg tagtcagata
     3181 actaaaatac ggggtttttt ctcaatttgt tatgtcatta ttttgggggg cgggcgggat
     3241 ggctcgtaac ttattgataa tcaataaagc ctcatccaat tatttaggag gttcagctgg
     3301 ttgcgacccc cgttcgtccc gtagaaaatc cttttggtgt atctacttat acgcggtgca
     3361 tatttgttaa aatatcagcc ggggtctttt ttcaggggcc ggcttggcat gtttgtagat
     3421 tagggtaccg gtatgaaagg caatgtgact cataccatct tctttatcta cgacgtcagg
     3481 gaccatgcct tgaaattctc cctgaaggca gaggttgtct gcaacgacgc cggggtgtat
     3541 accgtcagca atatccggcc ggagtcttcg gataatgacg gcgtattgct gccgcctatt
     3601 cagttgaaga aggagaatgg gggctggatc tttttggata atgggcagga gagcattctg
     3661 agcaatacca tcggccgggc gatagaggac agtcttatcg gggaaaatca tacccaccgt
     3721 cattctccta aacaatgatt cccagtcttt ttaagcgtac gga
//
LOCUS       DATGFG010000424         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1450999, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000424 DATGFG010000000
VERSION     DATGFG010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450999"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VKQ52_07935"
     CDS             complement(<1..534)
                     /locus_tag="VKQ52_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ87153.1"
                     /translation="MGLIRNALRKPITILVLVAGLLFFGIGAVRTIKIDIFPDLNLPV
                     IYVSHPYGGFTPDQMETYFGKQYVNLLLYVGGVKSIETRNIQGLTLIKLTFYEGTNMA
                     QAAAEVTGYTNRAQAIFPAGSQPPFILRFDASTLPVGQLVLSSPIRSNNELQDLANIY
                     VRASFSAVPGLVSPAPFG"
     gene            complement(552..1952)
                     /locus_tag="VKQ52_07940"
     CDS             complement(552..1952)
                     /locus_tag="VKQ52_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ87154.1"
                     /translation="MSKQQLTGLLGIFLLGGLPGAAGAQVLTLKEAVQTALNNYGTIR
                     AKINYVKASQANVKETKLEYLPDLNLSAQNVYGTVNSQFGPLASYKVSGVSSSGPVFA
                     TQNWNAAFGALYLTNVNWDFFQFGRAKEKTRVAQTVLDQNRSDLDQERFQQSVKVSAA
                     YLNVLAAQKLIISQQHNLDRAVAFQTVAKARARTGLNPGVDSSLANAEVSNARIALTN
                     AIEYEQEQANQLAQLMQVPPPVDWALDTLFLAKIPASLTDTPARSLDQHPLLQFYRQR
                     INVSKEEAKYLKTFTYPTFSLFGVLQDRGSGFGSGYAQYPDSYTANYFKGVSFDRGNY
                     LLGVGFVWNLTNPLRVHQQVASQQYISQGLQEEYGLVNQELQAQLTLAATRIRNALDN
                     YREAPIQVRAASDAYLQKETLYRNGLATIVDVTTAAFILNQAETERDIANSNVWQALL
                     FKAASSGDFGLFFNEF"
     gene            complement(2017..3429)
                     /locus_tag="VKQ52_07945"
     CDS             complement(2017..3429)
                     /locus_tag="VKQ52_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ87155.1"
                     /translation="MRLTTKFTLWYFGIMLVVLLIGGAIVYYEIQWKISRVEVVRHQR
                     LNDLIAQQIRQGGDYRDHPTRKRATITVIPADSAPKDLSSFYTRGIDWNPEFQRNEFK
                     LVVTSFYLIGGRYYRVTTYSFIPSFYQLLPGVVDSFKWILLVLLVLVIVSGGLISKYI
                     LAPFKRTMRVIQSFDLKQKEAIRLPNTQTSEFRELNQFLRKMTDKAREDYQSLKEFTE
                     NASHELQTPTAVIRGKLDLLMESDIRDEQAILIAEMQNALERLSRIHSSLTLLTKLEN
                     KEYEAKEPVCISNLMRETLDSFDELIQMKSITLESHVEKGVYIPLHLSLADLLITNLI
                     SNSIRHNAAAGAKGGRDGEEGSVGGSPGRGWIRVNLGREGLVVVNTGRQPQVPTQELF
                     ERFKKGNSGSDSIGIGLAIVRQICELSKFDIVYEYRDGMHLLAVSFQPRTPASKLLQN
                     LSLPLHLDAAEGRPDRQVRH"
     gene            complement(3426..>4130)
                     /locus_tag="VKQ52_07950"
     CDS             complement(3426..>4130)
                     /locus_tag="VKQ52_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87156.1"
                     /translation="YPNFGRMKVLIIEDNQELARNIRDFLGREGYICESSETMEQARE
                     KLTLFQYDCILLDIGLPDGNGIGLLNLIRSEHKESNVLIISAKNALDDKIIGLEKGAD
                     DYITKPFHLAELHARIRAVYRRKNLGGASIVEFNEISLNTDTFEARINNELLDTTPKE
                     FDLLLYFIVNKNRVLSRQAIAAHLWGDYTDNLANFDFVYQHVKNLRRKISNAGGMDYI
                     STVYGLGYKFNSHKQE"
BASE COUNT          902 a         1151 c         1131 g          946 t
ORIGIN      
        1 cccgaaaggc gcgggcgaca cgagaccggg aacagcgctg aaggaggccc ggacatagat
       61 attcgccagg tcctgtagtt cattattgct gcggatggga ctgctgagca ccagctgccc
      121 caccggcagg gtcgaggcgt caaaacgcag gataaagggc ggctgtgagc ccgccgggaa
      181 aatagcctgc gcccggttgg tataccccgt cacttccgcg gccgcctgcg ccatattcgt
      241 cccctcataa aaggtgagct tgatcagggt caggccctgg atattcctgg tctcgatact
      301 cttgaccccg cctacataca gcagcaggtt gacatactgt ttgccgaaat aggtctccat
      361 ctggtcgggg gtaaacccgc cataaggatg cgaaacgtag atgaccggca ggttcagatc
      421 ggggaagata tcgatcttga tcgtcctgac ggcgccgata ccaaagaaaa gcaaacccgc
      481 caccagcaca agaatggtga tcggttttct aagcgcattt ctgattaatc ccatggttgg
      541 agcagttaca gttaaaattc attaaagaac aagccaaaat ccccgctgga cgccgccttg
      601 aaaagaagcg cctgccacac gttgctattg gcgatatccc tttccgtctc ggcctggttg
      661 agaataaaag ccgccgtcgt cacatcgaca atggtggcca gtccgttccg gtataaggtt
      721 tccttttgca ggtaggcatc cgatgcggcc cttacctgta tcggcgcctc ccggtaatta
      781 tccagggcat tcctgatccg cgtggcggcc agcgtcagct gcgcctgcag ctcctggttg
      841 acaagcccgt attcctcctg cagtccctgg gaaatatatt gctgtgacgc cacctgctga
      901 tggacacgga gcggattggt cagattccat acaaaaccta cccccaacag gtagttgccc
      961 cgatcgaaac ttaccccctt gaaataattg gcggtatacg agtcgggata ctgggcatag
     1021 ccggagccga agccggagcc gcgatcctgc agcacgccaa acagggaaaa agtcggataa
     1081 gtaaaggtct tcaggtattt cgcttcttcc ttgctgacgt tgatgcgctg ccggtagaac
     1141 tgcaggaggg gatgctggtc gagcgaacgt gccggcgtat cggtgagtga agcaggtatc
     1201 ttcgcgagga aaagcgtgtc gagggcccag tccacgggcg gcggcacctg catcagctgc
     1261 gctaactgat tggcctgctc ctgctcgtat tctatcgcat tggtaagggc gatacgcgcg
     1321 ttggagacct cggcattggc cagggaagaa tccacgccgg gattcagccc cgtcctggcc
     1381 cgggctttcg ctaccgtctg gaaggccacg gcgcggtcca ggttgtgttg ctgggatatg
     1441 atcagcttct gagcagccag cacattgaga taggccgccg ataccttgac gctttgctga
     1501 aaacgctcct ggtcgagatc gctcctgttc tgatccagca cggtctgcgc caccctcgtc
     1561 ttctctttgg cacggccaaa ttgaaaaaaa tcccagttca cattggtgag atacaacgcg
     1621 ccaaaggcgg cattccagtt ctgcgtggcg aataccggac ccgaagagga cacacccgaa
     1681 actttgtagg atgccagcgg accgaattgg ctgttgacgg tgccatagac attttgcgcg
     1741 gaaaggttca gatcgggcag gtattccagc tttgtctctt tcacgtttgc ctgcgaagcc
     1801 ttgacataat tgatcttggc gcggatggtc ccatagttgt tgagcgccgt ctggacggct
     1861 tctttcaagg tcagaacctg ggcgccggcc gcccccggca gaccgcccag taaaaaaata
     1921 ccaagcaagc cggtcagttg ctgttttgac atgataatga gctgttttta aaaaattcct
     1981 ggctggataa gctagcctat ggcagcaaag cgatagctaa tgtctgacct gtcggtcggg
     2041 gcggccttcg gccgcatcca ggtgtaaagg gagcgaaaga ttttgaagta attttgaagc
     2101 cggggtacgc ggttggaaac tgacggcgag caggtgcata ccatccctgt attcgtagac
     2161 aatgtcgaat ttactcagtt cgcagatctg gcggacgatc gcgagcccga tgccgatgga
     2221 gtcgctgccg gaattccctt ttttgaaccg ttcgaataat tcctgcgtgg gaacctgcgg
     2281 ctggcggccg gtattgacga cgacgagccc ctccctgccc aggttgaccc tgatccatcc
     2341 gcgtccgggg gatccaccca ccgacccctc ctctccatca cgcccccctt tggcgcccgc
     2401 cgccgcattg tgccggatcg aattactgat caggttggtg atcagcaggt cagccagcga
     2461 caggtggagc ggaatatata cccctttttc gacatgcgac tcaagggtga tcgatttcat
     2521 ctggatcagc tcatcaaagg aatccagcgt ttcccgcatg agattgctga tacagaccgg
     2581 ctccttcgcc tcgtactcct tgttctcgag tttggtgagc agggtcagcg aactgtggat
     2641 ccgcgaaagc cgctccagcg cattctgcat ctcggcgata aggatggcct gctcgtcgcg
     2701 gatatccgac tccatcagca gatctaattt accccttatg acggccgtgg gcgtctgcag
     2761 ttcgtgagag gcattctcgg taaattcttt caacgactgg tagtcttccc ttgccttatc
     2821 ggtcatcttc cggaggaact ggttcagctc ccggaactcc gaggtctgtg tgttgggcag
     2881 acgaattgcc tccttttgct tgagatcgaa tgattgtatc acccgcatgg tccgcttgaa
     2941 gggagcgagg atgtatttcg agatcagccc gcccgagacg atcaccagca ccagcagcac
     3001 gagcaggatc cacttgaagg agtccacaac tcccggtagt aattgataga acgaagggat
     3061 aaaggaatag gtcgtgaccc ggtagtaccg gccaccgata agataaaagg aggtgaccac
     3121 cagtttgaac tcattccgct ggaactcggg gttccagtcg atgccccggg tataaaagga
     3181 actgaggtcc tttggcgcgg agtcggccgg tataacggtt atcgtagccc ttttccgcgt
     3241 cgggtgatcc ctgtagtcac ccccctggcg gatctgctgg gcaataaggt cattcagccg
     3301 ctggtgccgg accacctcca cccggctgat cttccactgt atctcgtaat agacgatggc
     3361 gcccccgatg agcagcacca ccagcatgat accgaaatac catagcgtga attttgttgt
     3421 tagcctcact cctgtttatg tgaattgaat ttatatccca gtccgtagac cgtactaata
     3481 tagtccattc cgcccgcatt gctgatcttg cgacgcaggt tcttgacatg ctggtaaaca
     3541 aaatcaaaat tagcgagatt atccgtgtaa tccccccaca aatgcgccgc gatggcctgc
     3601 cgggaaagga ccctgttctt gttgacgata aagtaaagca gcagatcgaa ctcctttggc
     3661 gtcgtgtcca gcagctcgtt gttgatccgc gcttcgaagg tatccgtatt caggctgatc
     3721 tcgttgaatt ccacgatgct ggcgcccccc aggttctttc ggcgatacac ggcccggata
     3781 cgggcatgca gctcggcaag atgaaaaggt ttggtgatat agtcgtcggc ccctttttcc
     3841 agcccgatga tcttgtcatc gagcgcgttc ttcgccgaga tgatcaggac attgctttcc
     3901 ttgtgctcgc tgcggataag gttcaacaac ccgatgccgt tgccatccgg caggccgatg
     3961 tccagcagga tacagtcata ctggaaaagc gtgagcttct ccctcgcctg ctccatggtc
     4021 tcgctcgact cgcagatata cccttccctg ccgagaaagt cgcggatatt gcgggccagc
     4081 tcctggttgt cttcgatgat cagcaccttc atccgcccaa aattcggata
//
LOCUS       DATGFG010000425         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_709636, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000425 DATGFG010000000
VERSION     DATGFG010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709636"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1576)
                     /locus_tag="VKQ52_07955"
     CDS             complement(80..1576)
                     /locus_tag="VKQ52_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87157.1"
                     /translation="MKKLYSIVVSLLAIAAHAQAPAPDTVTVDWGKTQMISKTTLTLQ
                     VVVNPPLRRGTPMHDSIFSSLKALGCDFVRYVPWLPYPRLGVAELYPPTSQKTSWDFS
                     VIDPMTLDFLEATRGHSVILNFSTIPAWMYTTKQPVTFPDDPYAVFWDYTQGSIPRDT
                     SYGEIANYYARLYSWYTRGGFTDELGKRHESGYHYAVPYWEVLNEVDFEHGNNARTYT
                     KLYDAVTDAIKKESPGTKFVGMALADPNRPEWFEYFLDPKHHRGGAPLDMISYHFYAT
                     GSVAAQGLDVMQYTFWDRADGFLNVAKFVESIRKRLSPTTKTTIDELGSILDNDNVVP
                     DRTDPIPGGYWNLSGALYAYTYLGLARLGIDIVGESQLVGYPTQYPSVSMINWKTGRP
                     NARYWVLKLLKDNFGPGDKFVNSGIAGSEPGAADAITLQAVVTARGNRLLLINKRNKA
                     VKVVLPREFIGATLSVVDGSTGDERPAKVAIAGSAIELQPFAVAVAGN"
     gene            1697..>2580
                     /locus_tag="VKQ52_07960"
     CDS             1697..>2580
                     /locus_tag="VKQ52_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87158.1"
                     /translation="MKLGLFAIGLDTYWPQFEGLEERLLDYLRIVEKQLAALHPDIVN
                     AGLVDTPDKAFAAGRLFRSADVGLIFLYVTTYALSSTVLPVVQAAGVPVVILNLSPSS
                     AIDYARFNALRDRTQMTGEWLAHCSACPVPEIVNVFHRVGIRSYQVTGMLERDEVCRA
                     DISEWVSAARVAHTMRYNRLGLMGHYYSGMLDVYTDLTQQYASFGGHIELLEVEELSA
                     LRKAVDPAAAARRVQLFREVFDIRPECSSSDLLQAAITSVALEQLVAKYKLGSLAYYY
                     KGTGNPDNEQAITSIILG"
BASE COUNT          551 a          739 c          732 g          558 t
ORIGIN      
        1 tagcgggtct cccacattct ttggcggagc cgccatcctc cccgaccacg ccaacccatc
       61 tgctccccgc cacggcccct caattccccg ccaccgccac ggcaaagggt tgtaactcga
      121 tcgccgaccc cgcaatcgca accttcgccg gcctttcgtc gccggtagac ccatccacca
      181 cgctgagcgt cgccccaatg aactcgcggg gcagcacgac cttcaccgct ttattccgtt
      241 tattgatcag cagcagcctg tttcccctgg cagtgacaac agcctgtaac gtgatcgcgt
      301 ccgcagcgcc gggttcactg ccggctatcc cactattgac aaatttatcg cccggcccaa
      361 aattgtcttt caatagtttg agtacccaat accttgcatt gggccggccg gtcttccagt
      421 tgatcatgct gacgctggga tattgcgtgg gatagccgac caactgggat tcgcccacga
      481 tgtcgatgcc cagtcgcgcc aggccaaggt aggtataggc gtagagcgct cccgacaggt
      541 tccagtaacc acccggtatg ggatccgtcc tgtccggaac gacgttatcg ttgtccagga
      601 tgctgccgag ctcatcgatg gtcgtcttcg tcgtcggcga gagtcgtttg cggatgcttt
      661 cgacgaattt ggcgacattg aggaagccgt ccgcgcggtc ccaaaaggta tactgcatga
      721 catcgagacc ctgggcggcg acactccccg tagcatagaa atgataggag atcatatcca
      781 gtggcgcccc gccccgatga tgtttgggat cgaggaaata ttcgaaccac tccggccggt
      841 tcggatcggc aagcgccatc cccacgaatt tggtcccggg ggactctttt ttgatggcat
      901 ccgtcacggc atcgtagagt ttcgtatagg tcctcgcgtt gttgccatgc tcgaagtcta
      961 cttcgttgag cacctcccag tagggaacgg cataatgata accggactca tgccgttttc
     1021 ccaactcatc ggtaaatccg cccctggtat accagctgta cagccgggca taatagttgg
     1081 cgatctcccc ataactggta tctcgcggaa tagaaccctg cgtataatcc cagaagacgg
     1141 cataggggtc gtccgggaaa gtaacgggtt gtttggtggt atacatccag gccgggatcg
     1201 tactaaagtt caggatgacg gaatgtcccc tcgtcgcctc gaggaaatcg agggtcatgg
     1261 ggtcgatcac gctgaagtcc caggatgtct tctgactcgt cggcggatat agctcggcca
     1321 cgcccagcct gggataaggc agccagggga catagcggac aaaatcacaa cccaatgcct
     1381 tcagggacga aaagatagag tcatgcatgg gagttccgcg gcgcagcggg gggttgacaa
     1441 caacctgcag ggtcagcgtt gttttcgaga tcatctgggt ctttccccaa tcaacggtga
     1501 ccgtatccgg cgccggtgcc tgggcatggg cagcaatggc caggagcgat actacgatgg
     1561 aataaagctt cttcattgac taaagttcgt caaccgcccg ggatggccaa gcgccggcaa
     1621 acgaaatcgc aaaaaggata tctcttgtcg caaaaaggac atttatggtc cggggcaggg
     1681 aaatagcttt actaccatga agctcggttt attcgccatc gggttggata cctactggcc
     1741 ccaattcgaa ggactggaag aaagattgct ggactatctg cggatcgtcg aaaaacaact
     1801 ggcggcattg cacccggaca tcgtaaacgc aggattggta gatacgccgg ataaggcttt
     1861 tgcggcggga aggcttttcc gatcggcgga tgtcgggctc atcttcctct atgtaaccac
     1921 ctatgcgctt tcgtcgacgg tgcttccggt agtccaggcg gccggggtgc cggtggtgat
     1981 cctgaacctg tcaccttcgt ccgcaatcga ttacgcccgt tttaatgcgc tccgggaccg
     2041 gacgcaaatg acgggggaat ggctggccca ttgttcggcc tgccccgttc cggagatcgt
     2101 gaatgtgttc caccgcgtcg gtatccgatc ctaccaggtc accgggatgc tggagcgcga
     2161 cgaggtatgc cgggcggaca ttagcgaatg ggtctcggcc gccagggtcg cccataccat
     2221 gcggtacaac cggctggggt tgatggggca ttactatagc gggatgctgg atgtctatac
     2281 cgacctgacc cagcaatacg cttccttcgg cggacatatc gagctgttgg aagtagagga
     2341 gctttccgcc ctgagaaagg ccgtcgatcc tgccgccgcc gcccgccggg tacaactatt
     2401 ccgggaagta ttcgatatcc ggccggaatg ttcgtcatcc gatctgctac aggcggcaat
     2461 cacgtcagtc gcgttggagc agctggtggc gaaatacaaa ttgggttcgc tggcctacta
     2521 ttacaagggt acgggcaatc ccgacaacga acaggcgatc acctctatta tcctggggaa
//
LOCUS       DATGFG010000426         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1392355, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000426 DATGFG010000000
VERSION     DATGFG010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392355"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VKQ52_07965"
     CDS             complement(<1..282)
                     /locus_tag="VKQ52_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ87159.1"
                     /translation="MSKIRCIIVEDEPLAVKVLADYIADTPFLELQGTFKDAILATEF
                     LRRESTDCIFLDIHLPRLKGMAFLRTLTQPPAVIITTAYHQYAVEGFDLN"
     gene            complement(275..1309)
                     /locus_tag="VKQ52_07970"
     CDS             complement(275..1309)
                     /locus_tag="VKQ52_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ87160.1"
                     /translation="MKPLFDKKRITLYTWIALAFWGLCSFGDLAEYLAAGEAGKRIIN
                     DTWRLIPVLVLNYILFEYTIPRLSWKRPGTGFLLIFGHIFLYSEGLYGWRQLGVAMGF
                     YTPFPAGSHDVSPQMGFSMGAVFFFGIIRYINNHNQLKKRAQQLQIEKQQAELNYLKS
                     QTNPHFLFNTLNNIYSLARDKSDLAPESVLRLSKILRYMLYEAGGQYIAIEQELKIIG
                     DYISLERLRYDDSLRINFNYDVEDMKQSLPPLLLIPLVENAFKHGVSETRHQPYIDIH
                     LSVEKRQLAFYVRNSTEEPAIDGKVRENIGLSNLRRQLELLYTDYTVSLEQKAAEFSA
                     TLKINLASHV"
     gene            1498..2160
                     /locus_tag="VKQ52_07975"
     CDS             1498..2160
                     /locus_tag="VKQ52_07975"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HLZ87161.1"
                     /translation="MNRTIQFGENVPGYNIRVLNEREIRAAAGILFLATFLSLMLILF
                     TNNFVPVKYVITIFFIDFIIRVFISPRFSPTLIIGRWIVSRQTPEYVGARQKKFAWAV
                     GLALSATMFVFLVLVNAYSPITGITCLVCLIFLYFESVFGICLACKFYSLVFRKKAQY
                     CPGEVCEVKAKQDIQRISAPQWLVVFFFAALLSLLISFGNGFYSKKPYDLFGLHTTAN
                     RK"
BASE COUNT          525 a          720 c          549 g          517 t
ORIGIN      
        1 attcagatcg aacccctcca cggcatactg gtgataggcg gtcgtgatga tcaccgccgg
       61 cggctgggtc agcgtgcgca gaaaggccat tcccttgagc cggggcaggt ggatatccaa
      121 aaagatacaa tccgtgctct cccttcgcag gaactcggtc gccaggatgg catccttgaa
      181 tgttccctgc aattccagga agggggtatc cgcgatataa tccgccagca ccttcaccgc
      241 cagcggctcg tcttccacta tgatacatct tatcttagac atgactggcc aggttgatct
      301 ttaatgtggc ggaaaattcg gccgccttct gttccagcga taccgtatag tcggtgtaca
      361 acagctcgag ctgccgccgt agattggaaa gcccgatatt ctcccgcacc ttcccatcga
      421 tggccggctc ctccgtcgag ttccggacat aaaaagccag ctgccgcttc tccaccgaaa
      481 gatggatatc gatatagggc tgatgccgcg tctcggaaac cccatgctta aaggcattct
      541 ccaccagcgg gatcagcaac agcggcggca gcgactgctt catatcctcc acatcgtaat
      601 tgaagttgat ccgcagcgag tcatcgtacc gcagcctctc cagcgagata taatcgccga
      661 tgatcttcag ctcctgctcg atggcgatat attggccgcc ggcctcatac aacatatacc
      721 gcaggatctt cgacaaccgt aacaccgact ccggcgccag gtccgacttg tccctggcca
      781 acgaatagat attattgagc gtgttgaaca ggaaatgcgg gttggtctgt gatttaagat
      841 agttcagttc ggcctgctgc ttctcgatct gcaactgctg cgctcttttc ttcagctggt
      901 tatgattgtt gatataccgg atgatcccaa agaaaaagac ggcccccatg ctgaacccca
      961 tctgcgggga cacatcgtgc gaccccgcgg ggaaaggcgt atagaacccc atcgcgacac
     1021 ccagctgccg ccagccgtac aaaccctccg aatacaaaaa gatatgccca aagatcagga
     1081 ggaaccccgt tcccggccgc ttccagctca gcctcgggat cgtatactcg aaaaggatat
     1141 agttgaggac aagaacgggg atcagccgcc aggtatcatt gatgatcctc ttccccgctt
     1201 ccccggccgc caggtactcc gccaggtcgc caaatgaaca gagcccccaa aaagcaagcg
     1261 cgatccaggt atagagggtg attcgttttt tatcgaaaag cggcttcata cgttcgtgaa
     1321 cacgggactc catccgggga gccgatacgc tgctaaatat cgggaaatat tttcacgccg
     1381 ctaaataccc ctcattggtt gacccgaacc cgcatcccat agaccaaaat cgatcgtctg
     1441 ttgatctaac ttgcagttcc tgaattaatt aggtagtatt atgaaaaact cagcacgatg
     1501 aaccgcacca tccagttcgg cgagaacgtc cccggttaca acatccgggt attgaacgaa
     1561 agggagatca gggccgccgc cggcatcctc ttcctcgcca ccttcctttc ccttatgttg
     1621 atcctcttca ccaacaattt cgttcccgtc aaatacgtga tcacgatctt cttcatcgat
     1681 tttatcatcc gggtctttat aagcccccgg ttctcaccca ccctgatcat cggccggtgg
     1741 atcgtcagcc gccagacccc cgaatacgtg ggcgcccggc aaaagaaatt tgcctgggcc
     1801 gtcggcctcg cgctctccgc cacgatgttt gtcttcctgg tcctcgtcaa cgcctatagc
     1861 cccatcacgg gcatcacctg cctggtctgc ctcatcttcc tctacttcga atcggtgttc
     1921 ggcatctgcc tggcctgtaa attctattcc ctggtcttcc ggaaaaaggc ccagtactgc
     1981 cccggcgagg tctgcgaggt gaaagcaaaa caggacatcc agcggatctc ggccccccaa
     2041 tggctggtcg tcttcttctt tgccgccctg ctatccctgc tgatcagctt cggcaatggc
     2101 ttctatagca aaaagcccta cgatctcttc gggctccaca ccacggcaaa caggaaataa
     2161 cccacatgca caaacgcagc ccactcgaat cctggttctc ccccttccgg gcgaacatca
     2221 tcggccacga ccagtgtttc gattctcccc tgggccgcaa accattggtc tacgcggact
     2281 ggacggccag cggcagggcc tatcggccca t
//
LOCUS       DATGFG010000427         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1392309, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000427 DATGFG010000000
VERSION     DATGFG010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392309"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VKQ52_07980"
     CDS             complement(<1..806)
                     /locus_tag="VKQ52_07980"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HLZ87162.1"
                     /translation="MRRIMIGFLCLVLTVHATAQTVSGIVKDSITSVPVKLATVAIRG
                     PEGDSTSKETITDEKGRFVFGKLPQGVFEVTLSFIGYNTKKLRVTIPAGSKAIHLGTL
                     LLGPGAEMLTAVVVDSRKSLVEEKIDRTVYNAERDKTIGSGDATDVLRRVPLLSVDID
                     GNVTLRGSANIKVLINNKPSTISANNLADALKQIPADQIRSVEVITSPSAKYDADGSA
                     GIINIILKKNELRGVILNTDMAFGLRGSFLGLDGGYRNKKMDLSVGGFGRA"
     gene            983..2020
                     /locus_tag="VKQ52_07985"
     CDS             983..2020
                     /locus_tag="VKQ52_07985"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ87163.1"
                     /translation="MATPRHRNREILFHTAFWAVYISFTINHISSYQNGPQIHWGRVL
                     TSTLISVLYVAIISYLNYFYFIPAFLIRKKIGRFLAAFAISFALISWLRIEVEGLVFG
                     DSSREVRNMARTQLVVQAVISDLFIVLFVGLLRFASDWLELDSRRRELETENLSAELN
                     FLKAQVNPHFFFNTLNNLYYLATIKSDLAPLAISKLSEVMRYMIYDSNHSKVQLAKEI
                     EYMQHYIGLERLRLEEGVPLEFEVAGRTDILITPLILIAFLENAFKHGVSNGNDQSWI
                     KARLEVDETRLTFRIGNGKPGRTVTPTEEEGIGLRNVKRRLELSYPGRYTLDIEDGED
                     RFFVSLTIAQP"
     gene            2017..>2620
                     /locus_tag="VKQ52_07990"
     CDS             2017..>2620
                     /locus_tag="VKQ52_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ87164.1"
                     /translation="MKITCHIIEDEPMASSLLELYVKKLPLLELVAVSANPLRALESL
                     KAMPVDLLLLDIRMPEMTGLSLLKVLQKKPLVIITTAYSEFALESYDLDVVDYLKKPI
                     TFERFLKAIDKVEQRLQPLGAAKEKWGGADYIFIKEGTRFIKVMIGEIIFIEGLRNYV
                     AIHTTHQKIVSLQRLKSLEDQLPADKFIRVHNSYIVSKNAI"
BASE COUNT          601 a          752 c          671 g          596 t
ORIGIN      
        1 gcacgcccaa aaccgccgac ggaaaggtcc atcttcttat tccggtaccc gccatccagc
       61 ccaagaaagg aacccctgag cccgaaggcc atatccgtgt tcaggatcac cccccggagc
      121 tcattttttt tcaaaatgat attgatgatg cccgccgaac cgtccgcatc atattttgcc
      181 gatggggaag tgatcacttc aaccgacctg atctggtcgg ccgggatctg tttcaacgca
      241 tcggccaggt tattcgccga tatggtcgaa ggcttgttgt tgatcaacac tttgatgttc
      301 gcgctgcccc gcagcgtaac gttgccgtcg atatccaccg agagcagggg caccctcctc
      361 aacacatcgg tggcatcccc gctcccgatc gtcttgtccc tttccgcatt atagaccgtg
      421 cgatctatct tttcctctac cagggacttt cggctgtcga cgacaacggc cgtaagcatc
      481 tccgcgccgg gccccagcag cagggtcccg agatggatcg ccttgctgcc ggcggggatc
      541 gtgacccgca gctttttcgt attatagccg ataaaggaca gcgtgacctc gaagacgccc
      601 tggggcagtt tcccgaagac gaaccttccc ttttcgtccg tgatggtctc cttcgaagtg
      661 ctgtcgccct ccgggccgcg gatcgcaacg gtggcaagtt tcacgggcac actggtgatg
      721 gaatctttca cgatgccgga caccgtctgt gcggtggcat gcaccgtcag caccaggcac
      781 agaaaaccga tcattatcct tctcatacgg gtggtttata tcaggttata aaagaggcaa
      841 aatagccgaa aagccgggtg gaaatattgg gtttgtaccc ttcgtatcta aaatttgacc
      901 gatcatgaat aatacttgac ggatgcggga ggcgcggctc gggcaatttc accaattgca
      961 gtatcttgtc tgcctgtaaa ccatggcgac gcctcgacat aggaaccggg agatcctttt
     1021 ccatactgcc ttttgggccg tctacatctc ctttaccatc aatcatatca gctcttacca
     1081 gaatggtccg cagatccatt ggggccgggt cctgacatcg accctgatca gcgtgctgta
     1141 tgtggccatc atatcctacc tgaactattt ctatttcatc cccgccttcc tgatcaggaa
     1201 aaagatcggc cggttcctcg ccgccttcgc gatctccttc gcgctgatct cctggttgcg
     1261 gatcgaggtc gaaggcctgg tcttcggcga ctcttccagg gaggtgcgta acatggcccg
     1321 gacacaatta gtcgtccagg cagtgatcag cgatctgttc atcgttctgt tcgtcggatt
     1381 gctgcgattc gcttccgatt ggctggaact ggattcccgg cggcgggaac tcgaaacgga
     1441 gaacctcagc gccgagctga atttcctgaa agcccaggtg aatccccatt tcttcttcaa
     1501 taccttgaac aacctgtatt atctcgccac gatcaagtcc gatctcgccc ccctggccat
     1561 ctcgaagctg tcggaagtga tgcggtatat gatctatgac tccaatcatt ccaaggtcca
     1621 attggcgaaa gagatcgaat acatgcagca ttatatcggc ctggagcgct tgcgtctgga
     1681 agaaggggtg ccgttggaat tcgaggtggc cggccgcacc gatatattga tcactcccct
     1741 gatcctgatc gccttcctgg aaaacgcctt taaacacggt gtcagcaacg gcaatgatca
     1801 aagctggatc aaggcaagat tggaagtgga tgaaacccgc ctgaccttca ggatcgggaa
     1861 cggcaagccg ggaagaaccg tgacgccaac ggaagaggag ggcatcgggc tcaggaatgt
     1921 gaagcgaagg ctggagctca gctaccccgg aagatacaca ctcgacatcg aagacgggga
     1981 ggatcgtttt tttgtaagcc taaccatcgc ccagccatga aaatcacctg ccatatcatc
     2041 gaagacgagc ccatggccag cagcctgctg gagctgtatg taaagaagct gcccctgctg
     2101 gaactggtgg ctgtcagcgc caatcccctc cgggccctgg aatccctgaa ggcaatgccc
     2161 gtcgacctgc tcctcctgga catccggatg ccggagatga ccggcctctc gcttcttaaa
     2221 gtgctgcaaa aaaagcccct cgtcatcatt acgaccgcct attcggagtt cgccctggag
     2281 agttatgacc tggatgtagt cgactacctg aagaaaccca tcaccttcga gcgtttcctg
     2341 aaggccatcg acaaggtgga acaacggctg caacccctcg gggccgcaaa agagaaatgg
     2401 ggtggggcgg attatatctt tatcaaagaa ggcacccgtt tcatcaaggt catgatcgga
     2461 gagattatat ttattgaagg gctcaggaac tatgtggcca tccacacaac ccatcagaag
     2521 atcgtgagcc ttcagcgcct gaagtccctc gaagaccagc tcccggccga caaattcatc
     2581 cgggtacata attcctatat cgtttccaag aacgccatct
//
LOCUS       DATGFG010000428         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_479649, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000428 DATGFG010000000
VERSION     DATGFG010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479649"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..2859
                     /locus_tag="VKQ52_07995"
     CDS             178..2859
                     /locus_tag="VKQ52_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HLZ87165.1"
                     /translation="MMPTVTMDIRLSADAQKALTIAQGLAREQLHAAVGAAHLLRALL
                     HADVGLEQRLWQMDQDVYYIADWADTRMEGYRKAAAPKASLRGDEAMMAVLEEADTVR
                     ALQNEEEVGPMHLLVALSTPGVGFSFEQLKTFPLQRERLLQASDPVTALLPGAGRGGA
                     GGSGDAGGGAVPGGRGSAAGGASSSAASVAGAAAGVGVSGTVGVGDVVGGAGVGQALL
                     KYCRDKTTDAALGKMDPIIGREKELRRMAEILCRRSKPNVLLIGEPGVGKTAMVDGFA
                     LAIQAGRVPALLKGARLFELDNGALVAGASYKGEIEERLKAILSELRGFDKAILFIDE
                     LHGLLDKSGAAAGAANLLKPELARGAITLIGATTGEEYRKYIEKDEAFARRFEGLVLT
                     EPDAENAFRMMKAVLPRYAEHHGIAAEEEVLREAVRLAKRYVKDRRLPDAAIDLVDQA
                     MASLRMAVDTGEEGWKRQWEEFCAMCEGVAGEPAEEDGARGANDAAVAVGEGVKVGAG
                     DWRWHYEQLKQATSPVIWAGVAPEEDMIVMQDEEAMRREVEGVHERLHGLLPLTRTAL
                     QVQDIASIVADKTGIPVGKLQAQERERLLQLPELLRQRVVGQDQAISAISEAILESRS
                     GLSKPGQPIGSFFFLGPTGTGKTELAKALAGLLFQDERFMIRFDMSEFKEEHSAALLY
                     GAPPGYVGYEEGGLLVNRIRQQPYAVVLFDEIEKAHPSVFDIFLQIMDEGKLHDKLGR
                     QGDFSHALIIFTSNIASRYVTEAFARGGAPTHAELLEKMAGHFRPEFLGRLTEIVPFG
                     QLRPEMMEAIFAIQMKGLYASLAAQGVGLIVEPGAARLVAEMGFSPEYGARPLAGVIR
                     GQLRRPLSRMIVSGELAPGSVVRLDVDDDGRLSWERL"
     gene            2931..3383
                     /locus_tag="VKQ52_08000"
     CDS             2931..3383
                     /locus_tag="VKQ52_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87166.1"
                     /translation="MAIEPYGIGGVEVKTDAFEAFADIPQNRVLLAEKLTKDPPVKPD
                     IVTGLTDIAAVFEHFGPEVNMEFETEEGTLRRETLRFQGLEDFGTKGITAQSPYLNEL
                     NAKQQQYHKIIRQLKTNKLLKQALANKETKDNLIRSLQALIAELDGTH"
BASE COUNT          666 a          726 c         1330 g          666 t
ORIGIN      
        1 caacgggaag atgatgccgt tcacggagtt gtgcaacaag ttcaatgccc tgaagagcga
       61 gaagaggatc aggaagctgt cggtgcagct ggtgaagaac gacaagacga attgtatcat
      121 cgcgcttttt gtgaatctaa aagagaagga agggcttttt agtaaactat tctgatcatg
      181 atgcctacag taacgatgga tatccggctt agcgccgatg cgcaaaaggc gctgacgatc
      241 gcccaggggc tggcgcggga gcagctgcat gccgcggtgg gcgccgcgca tttgctaagg
      301 gcgttgttgc atgccgatgt ggggctggag cagcgcttgt ggcagatgga ccaggatgtg
      361 tattatatcg ccgactgggc ggatacgcgg atggaggggt atcgcaaggc cgcggcgccg
      421 aaggcgagcc tgcggggcga tgaggcgatg atggcggtgc tggaggaggc ggatacggtg
      481 cgggccttgc agaatgaaga ggaggtaggg ccgatgcatc tgctggtggc gttgagtacg
      541 ccgggggtgg ggttctcttt tgaacagttg aagacctttc cgttgcagcg ggaacgacta
      601 ctgcaggcga gtgatccggt gacggcgttg ctgccggggg ctggtcgcgg cggggcgggt
      661 ggtagtgggg atgcgggtgg tggcgcggtg ccgggtggcc gtggttcagc ggcgggtggc
      721 gctagtagtt cggctgcgag tgttgctggg gcggcggcgg gtgttggggt tagcgggaca
      781 gttggtgttg gcgatgtagt tgggggcgcg ggtgtcgggc aggccttatt gaaatattgc
      841 cgggataaga ctacggatgc ggcgctgggg aagatggacc cgatcatcgg gcgggagaag
      901 gaattgcggc ggatggcgga gatcctttgc cggcggagca agcccaatgt gttgctgatc
      961 ggtgaacccg gggtgggcaa gacggcgatg gtggatgggt ttgcgctggc gatccaggcg
     1021 ggccgggtgc cggccttgtt gaagggagcg cggctttttg agctggataa tggagcgctg
     1081 gtagcgggcg catcgtataa gggggagatc gaagagcgat tgaaggccat tttatcggag
     1141 ctgcggggtt ttgacaaggc catcctgttc atcgacgaat tacatgggtt gctggataag
     1201 agtggcgcgg cggcgggtgc ggcgaatctg ttaaaacccg agttagcgcg gggggcgatc
     1261 acgctgatcg gggcgactac cggggaggaa taccggaaat atatcgagaa ggacgaggct
     1321 tttgcgcggc ggttcgaggg gctggtgctc acggagccgg atgcggagaa tgcgttccgg
     1381 atgatgaagg cggtgttgcc gcggtatgcg gagcatcatg ggatcgcggc ggaggaggag
     1441 gtgttgcggg aggccgtgcg gctggcgaaa cgatatgtaa aggaccggcg gttgccggat
     1501 gcggcgatcg acctggtgga ccaggcgatg gcttcgctcc ggatggccgt ggatacgggg
     1561 gaagagggat ggaagcggca gtgggaggaa ttttgcgcga tgtgcgaggg ggtggcggga
     1621 gagccggcgg aagaggatgg agcaaggggc gcgaatgatg cggctgtggc agttggagag
     1681 ggggtgaagg ttggggccgg ggactggcgc tggcattatg agcagttgaa acaggcgacg
     1741 agcccggtga tctgggccgg ggtggcgccg gaggaggata tgatcgtgat gcaggacgag
     1801 gaggcgatgc ggcgcgaggt ggagggtgtc catgagcggt tgcacgggct cttgccgctg
     1861 acgcgaacgg cgctgcaggt gcaggatatc gcttcgatcg ttgccgataa gacggggatc
     1921 cccgtcggga aattgcaggc gcaggaacgg gagcggcttt tacagctgcc ggagttgttg
     1981 cggcagcggg tggtaggtca ggaccaggcg atcagcgcca tcagcgaggc gatactcgaa
     2041 tcgcggtcgg ggctttcgaa gccggggcag ccgatcggtt cgttcttttt cctgggcccg
     2101 acgggtacgg gcaagaccga gttggcgaag gcgctggcgg gtctgttgtt ccaggacgag
     2161 cggtttatga tccgcttcga catgtcggaa ttcaaggagg agcattcggc ggcgttgttg
     2221 tatggcgcgc ccccgggata tgtcggttat gaggagggcg ggctgcttgt caaccggatc
     2281 cggcagcagc cgtatgccgt ggtgttgttc gatgagatcg aaaaggcgca tccgtccgtg
     2341 ttcgatatct ttttgcagat catggatgag gggaagctgc atgacaagct ggggcggcag
     2401 ggtgatttct cgcatgcgct gatcatcttt acttccaata tcgcgagccg gtatgtgacg
     2461 gaggcttttg cccgtggggg ggcgccgacg catgcggaac tgctggaaaa gatggcgggg
     2521 catttccggc cggaattcct cggcaggctg acggagatcg tgccgttcgg tcaacttcgg
     2581 ccggagatga tggaggcgat cttcgcgatc cagatgaagg gtttgtatgc atcgctcgcg
     2641 gcgcaggggg tagggcttat cgtggagcct ggggccgcga ggctggttgc ggagatgggc
     2701 ttttcgccgg agtatggggc gaggccgttg gcgggggtga tccgcgggca gctgcggcgg
     2761 cctttgtcaa gaatgatcgt gagcggggag ttggcgccgg ggagtgtggt gcggctggat
     2821 gtggatgacg acgggcgcct gagctgggag cggttgtagc cagggaattg cccagaccgg
     2881 ccggcgtaag acaagtaaaa ccattttatt catatttaaa ctatcgcttt atggcaattg
     2941 aaccttatgg aatcggtggg gtcgaggtca aaacggatgc gtttgaagcc ttcgccgata
     3001 ttccacagaa ccgcgtcctg ctggccgaaa aactgacgaa ggacccgccg gtaaagccgg
     3061 atatcgtgac cgggctgacg gatatcgccg cggtgttcga gcatttcggc ccggaggtga
     3121 acatggaatt cgagaccgaa gaaggcacgc tccggcggga gaccctgcgc tttcaggggc
     3181 tggaggattt cggcacgaaa gggatcacgg cgcagagccc atacctgaac gagctgaacg
     3241 caaaacaaca gcagtatcat aagatcatcc ggcaattgaa gaccaataag cttttgaaac
     3301 aggccctggc gaacaaggag accaaggata acctgatcag gagtttacag gcgttgatcg
     3361 cggagttgga tggtacccat taataaac
//
LOCUS       DATGFG010000429         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_586616, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000429 DATGFG010000000
VERSION     DATGFG010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586616"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..>3125
                     /locus_tag="VKQ52_08005"
     CDS             319..>3125
                     /locus_tag="VKQ52_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ87167.1"
                     /translation="MRKLHCLLLGTLLLISLGAFAQSTQVTGKVLDPSGTPIPNASIR
                     IKGVKGGTSADADGSFKLKVPPNAVLIVSGVGYETTEIPVNGRTSLTFSLKPGNSALS
                     EVVVTALGVKKEKKELGYAVTTIGKQDVELRPEEDITRILSGKAPGVDILATSGISGS
                     GTNISIRGISTITGGPSAPLFVVDGVPFDASSNVNNSFQYGGGISSSSRFLDLDPSNI
                     ESVSVLKGLAAATLYGEAGRNGVILVTTKGGSNRRINKKSEITATQSIFANTVANLPD
                     YTNIYGGGFDLVPSAAFSNWGAKFKNPPLQVSYSPSLQAAFPQYVGKTQDYRAYNSVP
                     KFFRTGLVSTTSVNVAAAGANTTFNANYTYLSDQGFTPNNRVYKNNFSLGGTAKLANN
                     FTANAVVNFAITDMQTPTVGANGGGGSADVASVFGDLIFTPRSINLMGLPYQNPVNGS
                     SIYYRGGNDIENPRWTAANDLSADKTYRTFGTFGLRYDVVKGLNIAYRFGLDLYQEFQ
                     TLEVNKGGKQGGPSYAGGLYRNTDIRNSVLNNTFTINYNTSLATNFSLDATGGFDMRH
                     DSYAQSGLKSTQQIVFGLFDHSNFISHDIYDEGGADLDYRNEEIREGAFAQATLGYKD
                     FLYLTGDARNDWVSTLEPANRQQFYPGVSASFIPTSAFEFLKASKAINYLKLRLDYAS
                     SAHFPTPYNTRPYLNLSTNSFIDKSGNVVNVASIPNQVPNPNLKPELIQEEEGGIEGK
                     FFDSRISLDLTGYFRTAKNQILNRQLDASSGYSYTTINAGSVDNKGIELALGYTVIRN
                     KNWTWDLNANFALNRSKVHDLPADIKNIEVAGGGYTNLGGFAINGKPLGILQGSYTQR
                     DPKSGKRIVDANGYFLGSSDIGIIGDPTPKYKLTGISTLSYRSLSFRMQWDYTRGGSI
                     FSNTIRTMLARGVTRDTEFDRT"
BASE COUNT          807 a          914 c          768 g          636 t
ORIGIN      
        1 acaaggagaa attagtataa actctgtgtg actcaatgtt gacagtgaaa gacggccccg
       61 gtttctaccg gggccttatt tttttcgcca tcccgcagtt atctgataat gatcgcatta
      121 ggcggcaaca ttatttaaaa aaaattattt ataaaataaa tgttaaaaat atactaattt
      181 aaacttacca aagctgatgt cggattgaag tagggtggga attgtaagcc ctttattttc
      241 aatctggtag ttattacgtt aagcctcggt accaggccgg accataggga aacaattaac
      301 aatctataat aactacacat gagaaaactt cactgcctgc tattgggcac gctgctgctc
      361 attagtctgg gggcattcgc ccaatcgaca caggtgaccg gaaaggtcct cgacccatca
      421 ggtacaccga ttcccaacgc aagtatcagg atcaaaggcg tcaaaggcgg taccagcgcg
      481 gacgccgacg gctcctttaa actgaaggtc ccccccaacg ccgtcctcat cgtcagcggc
      541 gtcggctacg aaacgacgga aattcccgtc aatggccgaa cttccctcac gttttccctg
      601 aaacccggta actctgcgct cagcgaagtg gtggttacgg cactgggagt aaaaaaggaa
      661 aagaaggaat taggatacgc ggtaactacc atcggcaaac aggatgtgga attacggccg
      721 gaagaggata tcacccgcat cctgtccgga aaggcgccgg gagtcgacat cctggccacc
      781 agcgggatct ccggtagcgg taccaatatc agcatccggg gtatcagcac catcaccgga
      841 ggcccatcgg cgccgctgtt cgtcgtggat ggagtaccct tcgacgccag cagcaacgtg
      901 aacaacagtt tccagtatgg cggaggcatt tcctcgagta gccggttcct cgatctcgac
      961 ccctccaaca tcgagagcgt tagcgtattg aaagggctgg ccgcagccac gctatacgga
     1021 gaggccggtc gcaacggggt catccttgtc accacaaagg gcggctccaa ccggaggatc
     1081 aataaaaaat ccgagatcac cgctacccag tccatctttg ccaataccgt cgccaatctc
     1141 cccgactaca caaatatcta cggtggcggg ttcgacctcg tcccctccgc cgctttcagc
     1201 aactggggtg cgaagttcaa aaatccgccg ctccaggtgt cctattcccc ttcgctgcag
     1261 gcagccttcc cccaatatgt ggggaagacc caggactaca gggcttacaa cagcgtcccg
     1321 aaattcttcc ggacagggct ggtcagcacc acctccgtca atgtggccgc cgccggagcc
     1381 aacaccacat tcaatgcgaa ttacacctat ctgagcgacc agggatttac tcccaacaac
     1441 cgggtgtata agaataattt cagtctcggc ggcaccgcga aactggccaa caatttcacg
     1501 gcaaacgctg tcgtgaattt cgccatcacc gacatgcaga cccccaccgt cggcgcgaat
     1561 ggcggtggcg gcagcgccga cgtggcctcc gtgttcggag acctcatctt caccccccgg
     1621 agcatcaacc tgatgggcct gccttatcag aaccccgtca acggttcaag catctactac
     1681 cggggtggta acgatatcga aaatccccgt tggacggccg ccaatgacct ttctgccgac
     1741 aagacctatc gtactttcgg aacctttggc ctgcggtatg atgtcgtcaa aggcctcaac
     1801 atcgcctacc ggttcgggct cgacctctac caggaattcc agacactgga agtcaacaag
     1861 ggcggcaaac agggcggccc aagctacgca ggcggattgt atcgtaatac agacatccgg
     1921 aactctgtcc tgaacaatac cttcaccatt aattacaata ccagtctcgc taccaacttc
     1981 agcctggacg caacgggagg atttgacatg cggcatgact cctacgccca gtcagggctg
     2041 aagagcaccc agcagatcgt gttcggcctt ttcgaccata gcaatttcat cagccatgat
     2101 atctatgacg aaggcggcgc cgatctggac tatcgcaacg aagagatccg ggaaggtgct
     2161 tttgcccagg ctacgctcgg ctataaggac ttcctttacc tgaccggcga tgcccgcaac
     2221 gactgggtct ctacgcttga accggctaac cggcagcagt tctatcccgg cgtcagcgct
     2281 tcattcatac ctacttcggc atttgaattc ctgaaggcca gcaaggcgat caactacctt
     2341 aagctgcgcc tcgactatgc cagcagcgcg cacttcccga caccgtacaa tacccgcccc
     2401 tatctgaacc tctccacgaa ttccttcatc gataagagcg gcaacgtagt gaatgttgca
     2461 tccattccca accaggtgcc gaatcccaat cttaagcccg aactgatcca ggaagaagag
     2521 ggcgggatag aaggaaaatt cttcgacagc aggatctccc tcgacctgac aggctatttc
     2581 cggacagcaa agaaccagat cctcaacagg cagctggatg cctcctcggg atatagctat
     2641 acgacgatca atgccggctc ggtagacaac aaagggatag aactggcgct gggatatacg
     2701 gtcatcagga ataagaactg gacctgggac ctgaacgcca attttgcact caatcgcagc
     2761 aaagtacatg acctgcccgc tgacatcaag aatatcgaag tcgccggcgg aggctacacc
     2821 aacctgggcg gcttcgcgat caacggcaaa cccctcggca tcttacaggg cagctatacg
     2881 caaagggacc ccaagtccgg aaagcgcatc gtggatgcca acgggtattt cctgggctcc
     2941 tccgatatcg gtatcatcgg cgacccgaca ccaaaatata aactcacggg tatcagcaca
     3001 ctgtcctata gaagcctctc cttccggatg caatgggatt atacccgcgg cggatcgatc
     3061 ttctccaata cgatccgtac gatgctggcc agaggggtaa cgagggatac cgagttcgac
     3121 cggac
//
LOCUS       DATGFG010000430         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_510242, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000430 DATGFG010000000
VERSION     DATGFG010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510242"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1718
                     /locus_tag="VKQ52_08010"
     CDS             441..1718
                     /locus_tag="VKQ52_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLZ87168.1"
                     /translation="MTNHRPIVKTLKFSILLLSLFSLGNRLFAQDGKALFQTNCASCH
                     SPFKVIIGPALKGATERIPDKKLLHDWIHNNTKVLAAGNSYFTALYNTYNKTPMNTFP
                     NLSDVEIDAILKYVDAAQPPTTSPTGEPETGSSAAKAAESDNTLLYGILTLILAIIMF
                     VLLQVNASLKKLSDDKEGVPAAEPVPFYRNKVYIALLVLVLFVFGGYLVVQGAIGLGR
                     QQGYQPEQPIFYSHKVHAGINQISCLYCHSNALDSRHATVPPLNVCMNCHAAINDYTH
                     GPKLYREDGAEVDGTAEIQKLYSYTGYDPKTSTYNTANAKPVEWIKIHNLPDHVFFSH
                     AQHTKAGQVQCQTCHGPIQEMDEVKQFAPLSMGWCINCHRTTKVNFPDSAGNNGNKFY
                     SIYERFHKDLKTGEMDSVTVENIGGTECQKCHY"
     gene            2038..>2620
                     /locus_tag="VKQ52_08015"
     CDS             2038..>2620
                     /locus_tag="VKQ52_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87169.1"
                     /translation="MIPGVADYYATTYVSGGDVIPVVAKVRDGRPIKIEGNELSSFTK
                     GGTSARVQASVLDLYDTARLRYPVQIVDGKPQEVSTFDAFDKLVGDALAGAAGSSVVL
                     LTATITSPTTKQIISEFLAKYPGSRHVQYDADSYAGLLLANEASYGKRAIPSYQFDKA
                     KVIVSLGADFLGTWLSPVEFARGWGQGRKVNAKE"
BASE COUNT          661 a          731 c          651 g          577 t
ORIGIN      
        1 ttgcggcaat atccagtacg cccgcaccgg gtttgttaca gacgataagg gcgactttga
       61 tgtgggtgtg ctggcgaaaa tggtcaatga tcctggctgt atttgaccct gctcccgagg
      121 cgaagatggc gatctgctgc atgcgcaaaa ctacaccggg cgccgcgatt tcggtgacct
      181 tttttcctcg gtaaacgtgt tgacaatact atgacagaag gtcagaacga tttgacgtaa
      241 ttcgctgaaa cccacgtcca acgcgggaaa aaaaattttc agcatgttga tatattgtta
      301 aaatcctgac ttatttttgc aacctgttaa tgtgcaggaa acgcattttc aacaaaaaaa
      361 ccagtgtatt tcctgcacat tactaatgtc cgttcttcgg acagggagat ttttccacat
      421 ttatacaacc ttttgtatat atgaccaacc atagaccaat agtcaaaacg ctaaaattca
      481 gtatcctcct cctctctctg ttttcactag gaaataggtt gtttgctcag gacggtaagg
      541 cgttattcca gacgaattgc gcctcgtgcc acagcccttt taaggtgatc atcgggcctg
      601 ccctgaaggg ggcgaccgag aggatcccgg acaagaagct gttgcacgac tggatccata
      661 ataatacaaa ggtgttggct gcggggaact cctactttac ggcgttgtac aacacgtaca
      721 acaagacccc gatgaacact tttcccaacc tgagcgatgt tgagatcgat gcgatcctca
      781 agtatgtgga tgcggcccag cctccgacta cctcgcccac gggtgaacct gagaccggaa
      841 gttccgccgc caaggccgca gagagcgaca acaccctgct ctatggtatc ctgacgctga
      901 tcctcgcgat catcatgttc gtgctcctgc aggtgaacgc gagcctgaaa aagctctccg
      961 acgacaaaga aggggtcccc gccgcggaac ccgttccctt ctaccgtaat aaagtgtata
     1021 tcgccttgct tgttctggtg ctttttgtgt ttggcggtta ccttgtggta cagggcgcca
     1081 tcggcctggg tcgtcagcag gggtatcagc cggaacagcc gatcttctat tcgcataagg
     1141 ttcacgccgg gatcaaccag atcagctgtc tgtactgtca cagcaatgcc ctggacagca
     1201 ggcacgccac cgtaccgccg ctgaacgtct gtatgaactg tcacgccgcg atcaacgact
     1261 atacccacgg tccgaaatta taccgcgagg acggggccga agtcgacgga accgccgaga
     1321 tccagaagct atacagctac accggttacg atcccaagac gagcacctac aacaccgcga
     1381 acgccaagcc ggtggaatgg atcaagatcc acaatctccc cgaccatgta ttcttcagcc
     1441 atgcccagca cacaaaggcc ggccaggtac aatgccagac ctgtcatgga ccgatccagg
     1501 agatggacga ggtgaagcaa ttcgcccctc tcagcatggg ctggtgtatc aactgtcacc
     1561 ggactaccaa ggtgaacttt cccgactccg ccgggaacaa cggcaacaag ttctacagca
     1621 tttacgaacg gtttcataag gacctgaaga ccggcgaaat ggactctgtc acggttgaga
     1681 acatcggcgg caccgagtgc cagaaatgcc actattaatc ttttaccccc ctcctccccc
     1741 tggcggagtt cacatactaa gtttaacttc tcaaatatga gcgacaaaaa gtactggcaa
     1801 agtttcggag agcgcaataa cagtgaagcg tttcagaaat caatatcgac agacggagag
     1861 ttcggaccgg accccctccc tctggaaggc ctggatggaa aaggattgct cgatgccaaa
     1921 actccccgca gagattttct taaatattta ggattcagta ctgctgccgc cacattggtc
     1981 gccagctgcg aaatccccgt aagaaaggtc gttccttacc ttaataagcc ggaaaacatg
     2041 atccccggtg tggccgacta ttatgccacc acctatgtat ccggcggaga tgtaattcct
     2101 gtcgtagcca aagtcaggga cggccgcccc attaaaatag agggcaatga actgtcctct
     2161 ttcaccaagg ggggaacttc cgcacgtgtt caggcttccg tcctggacct ctatgacacg
     2221 gccaggctgc gctatccggt tcagatcgtc gacggcaagc cccaggaagt gagcacattc
     2281 gacgcttttg acaaactggt cggggatgcc ctggcaggag cggcaggctc gtccgtcgta
     2341 ctcctgacgg caaccatcac ctcgcccacc accaaacaaa ttatcagcga gttcctcgcc
     2401 aaatatcccg gaagccggca tgtacaatat gatgcggatt cctatgcagg tctcctgctg
     2461 gccaacgaag cttcttacgg caagagagcc atcccctcct accagtttga caaggcgaaa
     2521 gtgatcgtga gcctcggcgc cgatttcctc ggtacctggt tgtctcccgt ggaattcgcc
     2581 cgcggatggg gacagggccg gaaggtaaac gctaaagagc
//
LOCUS       DATGFG010000431         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1157792, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000431 DATGFG010000000
VERSION     DATGFG010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157792"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VKQ52_08020"
     CDS             <1..441
                     /locus_tag="VKQ52_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLZ87170.1"
                     /translation="IKVQTDSAVRKLAASYPYDHFEDERAAVTLSTNFDEVNTSLERE
                     TAERLEIAGIEVVEARITHLSYAPEIAGAMLKRQQASAVVAARHKIVEGAVGMVEAAL
                     HLLSAKQIIELDEEKKAAMVSNLMVVLCGDRESQPVVNTGTLNH"
     gene            444..644
                     /locus_tag="VKQ52_08025"
     CDS             444..644
                     /locus_tag="VKQ52_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87171.1"
                     /translation="MAEKKSFALRMDAATMKAIEKWAADEFRSVNGQIEWVLHRALKE
                     AGRLKETSENGDTGNNNKTNGD"
     gene            814..2718
                     /locus_tag="VKQ52_08030"
     CDS             814..2718
                     /locus_tag="VKQ52_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ87172.1"
                     /translation="MNPIYPICYPLTRIKNLFLLLLLLAGFSRAATAQAPINAYARVT
                     NISGTTLTLSNINQTYHTFAAGENIVIIQMQDTVIGADTANNTSFGTIGSIAHAGVYQ
                     PATISSIVGSTMVLTAGLTSTYNTTANARVQVVSFQSLSAGNYTLNTGVTAVPWDGNV
                     GGIIAFQVGGTLTLNANVSADAAGFRGGSVNVGYELGCESTVYYSTNGTKYAYKGEGI
                     YSTPTISYTLATGREPLINGGGGGSDDNGGGGGGGNFTAGGQGGHGWTCSGNPSGGFG
                     GVALGTYISGGRVFMGGGGGGGQQNNAVGTNGVAGGGIVFIKATQIKTTCTGSYSITA
                     AGASATTSGNDGAGGAGAAGTVVLGVSSYNISGSCPLTIASNGGNGGNVGDNNGHGGG
                     GGGGQGAVIFSSALPGAGSNVTATTNNGNGGANSAFYDNTYAGNGAGSNNAGIISGAT
                     VVLPVDFLSITATRSGQDNVVSFTTGQTLQAVQYTIQRSADGSQFSDIGTVLGVTSSD
                     ATESYAFTDALPNAGKNFYRVRSTDVSGGEKYSTVVSVDRSDKAGGFHIYPNPAHGAF
                     TIQLRDATMNGSVGIAIEDLSGATVYRSLMMANNGRVSVANIGSLTAGIYLIRVETQG
                     GVQTGKLILH"
     gene            2871..>3062
                     /locus_tag="VKQ52_08035"
     CDS             2871..>3062
                     /locus_tag="VKQ52_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87173.1"
                     /translation="MFKYPVTILLILLVATQTFSKWCLIAGFQVNRDYIASNLCVNRL
                     NAISCCKGKCFLNKSLADDE"
BASE COUNT          670 a          725 c          992 g          675 t
ORIGIN      
        1 ataaaggttc agacggattc ggcggtgcgg aaattagccg cttcctatcc ttatgatcat
       61 ttcgaggatg agcgggcggc ggtgaccctg agcacgaatt ttgacgaggt gaatacctcg
      121 ttggagcgag agacggccga gcggctggag atcgcgggca tcgaggtcgt ggaggcgagg
      181 atcacgcatt tatcgtatgc cccggagatc gcgggggcga tgctgaagcg gcagcaggct
      241 tcggctgttg ttgcggcgcg gcataagatc gtcgaagggg cggtggggat ggtggaggcc
      301 gcgctgcact tgttgtcggc gaagcagatc atcgagttgg atgaggagaa gaaggcggcg
      361 atggtgagta acctgatggt ggtgctttgt ggtgaccggg agtcgcagcc ggtggtgaat
      421 acggggacat taaatcatta agaatggcgg aaaagaaatc ctttgccttg cggatggatg
      481 ctgcgacgat gaaggccatc gagaaatggg cggcggacga gtttcgcagc gtcaacggcc
      541 agatcgagtg ggtgctgcac cgtgcgctga aggaagcggg gcggttgaaa gaaaccagtg
      601 agaacggaga taccgggaat aataacaaga caaacgggga ttaaaaaata ttatttaaat
      661 attaatatgt agtcgaaccc cgggtttata ccgatagacc ccggggtttt attttcagag
      721 gacgcatacg gaaatcggtt caattagcgt ttaagagcat agaaatttgg cctactatcc
      781 tgttagtgat tgaaaaccaa ccctgaacaa ccgatgaacc ctatttaccc tatctgttat
      841 ccgcttaccc ggataaagaa cctgtttctc ctcctgttgt tgctcgcggg attctcccgg
      901 gcagctacgg cccaggctcc gatcaatgcc tacgccaggg taacgaacat ttccggaaca
      961 acgcttactc tttctaatat aaatcaaact tatcatacgt ttgccgcggg cgagaatatc
     1021 gtcatcatcc agatgcagga tacggtgatc ggcgccgata ccgcgaacaa taccagtttt
     1081 gggaccatcg ggtccattgc ccatgcgggg gtataccagc ctgcgacgat cagttccatt
     1141 gtcggatcga cgatggtgtt gacggcaggt ttgaccagta cgtacaatac gactgcgaat
     1201 gcgcgcgtgc aggtggtgag tttccaaagt ctgagcgccg gcaactatac gcttaataca
     1261 ggggtgacgg ccgtgccctg ggatgggaat gtaggcggca tcatcgcgtt ccaggtaggg
     1321 ggcaccctta cgctgaatgc gaatgtcagc gccgacgcgg cgggattccg gggtggcagt
     1381 gtcaatgtcg gctatgaatt aggctgtgag tccactgtat attacagtac taacggcacc
     1441 aagtatgcgt ataagggtga gggtatctat agtacgccga cgatcagcta tactcttgcc
     1501 actggtcgtg agccgctgat caatgggggc ggagggggta gtgacgataa cggcggtggt
     1561 ggcggcggcg gcaactttac ggccggcggg cagggcggtc acggctggac ctgttccggc
     1621 aacccttcgg gaggatttgg cggtgtggcg cttgggacgt atatctccgg aggccgcgta
     1681 tttatgggcg gcggcggtgg cggcggccag cagaacaacg ctgtaggcac caacggggtg
     1741 gccggcggcg gcattgtctt tatcaaggcc acccagataa aaaccacctg caccgggagc
     1801 tacagtatca cggcggcagg ggccagcgcc accaccagcg ggaatgacgg ggctggcggg
     1861 gccggggcgg ccggaacggt cgtgctgggg gtgagttcct ataatatttc gggcagctgt
     1921 ccgttgacaa ttgcgtccaa tggcggcaat ggcgggaatg tgggagacaa taatggtcat
     1981 ggcggcggcg gtggcggcgg tcagggggcg gtgatctttt cgtccgctct tcccggcgcg
     2041 ggttccaatg tcacggcgac gacgaataac ggcaacggcg gggcgaacag cgccttctac
     2101 gataatacct atgcgggcaa tggcgccgga agcaataatg ccgggatcat ctcgggcgcc
     2161 acggtcgtgt tgcctgtgga ttttctttct attacagcga ccaggagcgg gcaggacaat
     2221 gtggtcagtt tcaccacggg tcagacgctt caggccgtgc agtatactat tcagcggtcg
     2281 gccgacggct cgcagttcag cgatatcggt acggtgctgg gggtcacgtc gagtgatgcg
     2341 accgagagtt atgcttttac cgatgcgctc ccgaatgccg ggaagaattt ttaccgggtg
     2401 cggtcgaccg atgtttccgg tggggagaaa tattcaacgg tggtgtcagt ggaccggagt
     2461 gataaagccg gcggcttcca tatctacccc aaccctgcgc acggcgcttt tacgatccag
     2521 ttgcgggacg cgacgatgaa cggatcggtc gggatcgcga tcgaagatct ttcgggcgct
     2581 acggtctatc gtagcctgat gatggcgaat aatggaaggg tgagtgttgc gaacatcgga
     2641 tcgctgacgg cggggatcta tcttatccgg gtagaaacgc agggcggtgt gcagacagga
     2701 aagctgatcc tgcattaggg atgatcctgc gttgatcgcc tggcatgtgg tcggcgtggc
     2761 tcgtatggct cgtatggccg gtgttttcct gttctgaaaa aaattgatgg gttagggctg
     2821 ttgtatgctc caacccattt acctttgccc ccggtgtact gtttcaccga atgttcaaat
     2881 atcccgtaac catattactc atcctgctgg tggccacgca gaccttcagc aaatggtgcc
     2941 tgatcgccgg attccaggtg aaccgggact atatcgccag taatctttgt gtcaaccgcc
     3001 tcaatgccat cagttgttgc aaggggaaat gttttctcaa caaatcgctt gccgatgatg
     3061 ag
//
LOCUS       DATGFG010000432         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_607057, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000432 DATGFG010000000
VERSION     DATGFG010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607057"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VKQ52_08040"
     CDS             <1..320
                     /locus_tag="VKQ52_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87174.1"
                     /translation="TIYLKKGIMKKIKIAAMPAAILLALILAWTTRRPPLGPKNPPPG
                     SKNFDCTGVQNYHFNGISYLPVGQFGIDYYCAEGGDVCTYYQVSPGVYAACRLGAYTP
                     IHH"
     gene            complement(364..1761)
                     /locus_tag="VKQ52_08045"
     CDS             complement(364..1761)
                     /locus_tag="VKQ52_08045"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87175.1"
                     /translation="MNMRSVLSPFLLVTLVLTGLAVGGCRKAFLSKLPSTDLVVPSTL
                     SDFQALLDNDAVMSGTPVLGELSADNFYLPYTFWETLDTREQNAYVWAKDIYQGQGLV
                     DDWDIPYKQVFYANVVLEGLSGITVDNTNRQQWQNEEGSALFLRAYAFFNLAQLFAPV
                     YDSLTAGTDPGIPLRLHSDITTPSIRASVKDTYTQILSDLQQASALLPAGVPAANRNR
                     PSKPAALAMLARVYLSMRAYSLAGLYADSCLQLYSTLIDYNTVSPQAYLPFTRLNPEA
                     IYQSSFLTYTHVLAALAFPSCIIDSNLYRSYAPNDLRSSIFYLINTNGQPNMKGGYSG
                     TIYPFSGLAADEVYLVRAECAARQGDTTRALQDLNTLLKNRWLQGTFTPVTAASPAAV
                     LDTILAERRKELAFRGLRWTDLRRLNMEGRNITLIRLLNGANFQLNPRSLNYVLPIPP
                     DVLAYNPKMNQNPRQ"
     gene            complement(1773..>3947)
                     /locus_tag="VKQ52_08050"
     CDS             complement(1773..>3947)
                     /locus_tag="VKQ52_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87176.1"
                     /translation="TNNHYYVSAGLDRDLTNLVGNRYNRITLTGANTYMLVPQKLEFE
                     LGMAFTSSLTYNNNTVSSSNSYPYPYARLADAQGNALAVSYNLRQSYIDTAGGGQLLD
                     WNYRPLNELRNANNVTRVNDYRFLTGFHYTFRPGLQGYALYQYSRGVSDQQDLQNIQT
                     YYTRNLINEFTQIDASGQVIRPVPLGDIFDELVNLYQAHNGRLQVSYDHSFFGGHELH
                     ILSGAEIQAVEGRIRLNRLYGYNPLSQSSLPVASYTISYPQYSSPGSSMQIPSLDHNA
                     ASSDHYLSSYLNGSYRFRQRYTATVSGRIDESNLFGVNTNKKVIPLWSAGLAWEISRE
                     QFYRIDWLPYVKLRATSGYNGNVYKSVSGYTTASASTAVSIDVAYQNLYGSSYADIIN
                     PPNPNLTWEKVHVFNLGLDFASKGKTVEGSVEYYSKNGQGLIGPTGVDPTTGNTQYTG
                     NAANMVTHGVDLTLRTSIRVGAIRWNSVVLFNYVQDRVTRYLVRPGTIFPFFGSGTIN
                     PLVGKPLYSVYALPWAGLDPQTGDPQGTSAGHASKDYTALIGSSDFGTMLYKGPVNPP
                     FFGSWRNELLWRRWGISVNIMYKFGDYFRRTSIQYAYLFAGSSLGHPDYTRRWQNPGD
                     EKHTYVPSMVYPASSARDAFYQNAEVLVEKGDFVRLQDLYLYYELPKKAIPKWPVQAM
                     RLYLYGNNIALLWRANRQGIDPDATLGIPTPRTLAVGLKLEL"
BASE COUNT          936 a          968 c         1115 g          928 t
ORIGIN      
        1 cgaccattta tctaaaaaaa ggtatcatga aaaagataaa aattgcggca atgcccgctg
       61 ccattctgtt ggctttaatc cttgcgtgga cgacacgtcg gccacctttg ggcccaaaaa
      121 acccacctcc gggttcaaag aattttgatt gcacaggggt tcaaaactac cattttaatg
      181 ggataagcta tttacctgtg ggtcaatttg ggatagatta ttattgtgca gagggtggcg
      241 atgtctgcac ttattatcag gtttctccag gagtttacgc agcctgtcgt ttgggagctt
      301 atacacccat acatcattag ctataaataa cgcgtatgcc tcttggaaac aatggactat
      361 cactcactgc cgaggatttt gattcatttt gggattgtag gcgagaacgt caggtggtat
      421 ggggaggacg taattgaggc tacgggggtt gagctggaag tttgcgccat tcaggaggcg
      481 aatcagggtg atgtttcgtc cttccatgtt gaggcgtcgc aggtccgtcc agcggagccc
      541 ccggaacgcc agctccttcc ttcgttcagc caggatggtg tccaggactg ccgccggcga
      601 ggcggcggtg accggggtaa aggtgccctg cagccagcga tttttgagca gggtattcag
      661 gtcctgcaac gcccgggtgg tatccccctg ccgggcggca cattcggccc ggaccaggta
      721 gacctcatcg gcggccaggc cgctaaaagg atagatcgtg ccactgtatc cccctttcat
      781 gttcggttgc ccattggtat tgatcaggta aaagatggaa ctacggagat cgttcggcgc
      841 gtaggatcga tacagattgg agtctatgat gcaactgggg aaggccaggg cggccagcac
      901 gtgggtatag gtaagaaaac tgctttgata gatggcttcg ggattgagcc gggtaaaggg
      961 cagatacgcc tgggggctga cggtattgta gtcgatcaag gtgctgtata gctgcaggca
     1021 gctatcggca tatagacccg ccaaggaata ggcccgcatg ctcaggtaga cccgggccag
     1081 catagccagg gcggccggtt tggaggggcg attgcgattg gctgccggca cccctgccgg
     1141 caatagggca ctggcctgct gcagatcgga gagtatctgg gtataggtgt ccttcacaga
     1201 ggcccgtatg gatggggtgg taatgtctga gtgaagacgc agcgggatgc ccggatcggt
     1261 gccggctgtc aggctatcat acaccggggc aaagagctgg gccaggttga aaaaggcata
     1321 tgcccgcagg aacaaggccg atccttcttc attttgccat tgctggcggt tggtgttgtc
     1381 caccgttatg ccggataagc cttccagtac cacattggca taaaacacct gcttatacgg
     1441 gatgtcccaa tcgtcgacaa gcccctggcc ctggtagatg tccttcgccc atacgtaggc
     1501 attttgctcc cgggtgtcca gggtttccca aaaggtgtac ggcaagtaaa aattatctgc
     1561 cgataactct cccagaacag gcgttccgga catgacggca tcattgtcca ggagggcctg
     1621 gaagtcactc agggtggaag ggacgaccag gtcggtgctc ggtagcttac tcaaaaaggc
     1681 tttccggcag ccacccacag ccaggccggt cagcaccagg gttactaata ggaaggggga
     1741 taatacactt cgcatattca tgagggaggg ttttaaagtt ccagttttaa accgacagca
     1801 agtgtccggg gagtggggat gcccagggtg gcgtccgggt cgatcccctg ccggttggct
     1861 cgccacaaca aggcaatgtt gttgccatat aaatagagcc gcatggcctg taccggccat
     1921 ttcgggatcg cctttttggg cagctcgtag tacaggtaga gatcctgcaa gcggacaaaa
     1981 tccccttttt caaccaacac ttctgcattc tgataaaagg cgtccctggc ggagctggcc
     2041 ggatagacca ttgagggtac atacgtgtgt ttctcgtcac caggattttg ccaacgccgg
     2101 gtatagtcag gatgaccaag ggaagaacca gcgaacagat aagcatattg gatagatgtt
     2161 cttcggaagt aatcgccgaa cttatacatg atgttgacag atattcccca tcgcctccat
     2221 agcagctcat tgcgccagct cccaaaaaaa ggggggttga cagggccttt atacaacatt
     2281 gtaccgaagt cggaagaccc gatcagtgcg gtatagtctt tactggcatg tccagccgag
     2341 gtcccctggg gatcacccgt ctgtggatcg agccctgccc agggaagggc atagacggaa
     2401 tacaggggct ttcccactaa cggattgatt gtcccagagc caaaaaacgg aaagatagtg
     2461 ccgggtctga ccaggtaacg ggttacccga tcctggacat agttgaacaa caccaccgag
     2521 ttccaccgga tagcaccaac acggatgctg gtacgcaagg tcaggtccac cccatgagta
     2581 accatattgg ccgcattccc ggtatactgt gtatttccgg ttgtcgggtc tactcccgtt
     2641 gggccaatca agccttgtcc atttttacta taatactcga cactgccttc caccgtcttt
     2701 cctttactgg caaagtccag ccccaggtta aacacatgca ccttttccca ggtaaggttc
     2761 ggattgggag ggttgattat gtcggcgtat gacgagccgt acaggttttg ataagcaaca
     2821 tctatcgaga ctgccgtgga tgccgacgca gtcgtataac cagagacaga tttgtataca
     2881 ttcccattat atccgctcgt tgcccggagc ttcacatagg gcagccaatc gattcgatag
     2941 aactgttctc ttgagatttc ccaggccagc cctgccgacc acagggggat cactttttta
     3001 ttggtattaa ctccgaacag attgctctca tcgatacgtc cgctgacagt agcagtatat
     3061 cgctggcgga accggtacga tccattcaaa taagaagaga gatagtgatc cgaactggca
     3121 gcattatgat ccagagatgg aatttgcata gaactgccgg gagatgaata ttggggatat
     3181 gagatcgtat aactagcgac cggcaggctg ctttgagaaa ggggattgta gccgtataac
     3241 cggttcaggc gtatccgtcc ctccaccgcc tggatctcgg cgccggacaa gatgtgcagt
     3301 tcatggccgc caaaaaagga atggtcatag ctgacctgca agcgaccgtt atgagcctgg
     3361 taaaggttga ccagttcgtc gaagatatcc cccaggggaa caggccggat aacttgcccc
     3421 gatgcatcga tttgcgtaaa ctcgttgatg aggttgcggg tgtaataggt ctggatattc
     3481 tgcagatcct gctggtcgct tactccccgg ctgtattggt agagtgcata tccttgcaac
     3541 cctggccgga aggtataatg aaaaccggtc aggaaccggt agtcattgac cctggtgaca
     3601 ttatttgcat tccgcagctc attcaatggc cggtagttcc agtccaatag ctgtccgccg
     3661 cctgccgtgt cgatatagga ttgtcgaaga ttgtatgaaa cagccagggc attaccctgg
     3721 gcgtcagcca gcctggcata aggataggga taggagttgc tgctgctaac cgtattattg
     3781 ttgtaggtaa gcgatgaggt aaaggccatg cccagttcga actccagctt ttgaggaacg
     3841 agcatatacg tattggcacc ggtcagggtg atccggttgt aacgattccc taccaggttg
     3901 gtcaggtccc tgtccagccc tgcagacaca taatagtggt tgttggt
//
LOCUS       DATGFG010000433         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1040373, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000433 DATGFG010000000
VERSION     DATGFG010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040373"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VKQ52_08055"
     CDS             complement(<1..534)
                     /locus_tag="VKQ52_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HLZ87177.1"
                     /translation="MSILVNKQSKIIVQGFTGTEGTFHATQMIEYGTQVMGGVTPGKG
                     GSTHLERPVFNTVADAVKTTGANVSIIFVPPAFAADAIMEAAEAGIALVICITEGIPV
                     QDMVKAKNYLLGKNTRLIGPNCPGVITAGECKVGIMPGFVFKKGKIGIVSKSGTLTYE
                     AADQVAKAGLGISTAVGI"
     gene            complement(610..1149)
                     /locus_tag="VKQ52_08060"
     CDS             complement(610..1149)
                     /locus_tag="VKQ52_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HLZ87178.1"
                     /translation="MQKFISLVLLAVFAGGASAQTFTLKSNDVGGQATNKQFANSFGC
                     HGENVSPELHWENVPAGTQAFAVTIYDKDAPTGSGFWHWVIFNIPANVTGLKSDAGTI
                     AKNLAPADAIQCNTDAGQPGYMGPCPPPGPIHEYVVTVYALKSKIPLDKTAGPALAGF
                     YLNANAIEKASIVFYAQNK"
     gene            complement(1218..2075)
                     /locus_tag="VKQ52_08065"
     CDS             complement(1218..2075)
                     /locus_tag="VKQ52_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ87179.1"
                     /translation="MDLFPVRDSPAFPGRTAIRIYDSAQSPSRTRVILKNNLFSFLLE
                     GEKTVHRPGEPIHIRGGQFLLLAGGNTLMTERLSVNGRYHSLLFFFDDSVFRDFWSRH
                     PGLPDAGKGRESLVAFTADDFIRNYLDSLQLMLAANPALQEDLQLLKFEELMLYLAGK
                     YPGLLHSFRLSDPHDPDHRQMMAVVEGNVNHAITVDELAFLCNMSLSTFKRRFARLYS
                     TSPNKWLLQRRMELAASMLDSQEKPSEVYHKVGYENHSSFTQSFKQVYGMTPREFQKR
                     AVRPLNVYP"
     gene            complement(2104..>2832)
                     /locus_tag="VKQ52_08070"
     CDS             complement(2104..>2832)
                     /locus_tag="VKQ52_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ87180.1"
                     /translation="KCNLIIGTSGSGKTVLMKCLVGLFEPDSGEIVYDGKNFSEMNEE
                     HRKELRQNIGMLFQGSALFDSMTVEQNIMFPLDMFTKNTPAEKLKRVNEVLERVNLEG
                     ANSKFPAEISGGMKKRVGIARAIVLNPRYLFCDEPNSGLDPQTSLLIDKLIKEITDEY
                     NITTVINTHDMNSVMEIGNHIMYMYQGHKEWEGNNKEIIFSKNKLLNDFIFASEFLQD
                     AKDMRMLEVKGKIDNNRNMDELLK"
BASE COUNT          631 a          802 c          722 g          677 t
ORIGIN      
        1 gatgcccact gccgtggaga tacccagacc agccttggcc acctggtcgg cggcttcgta
       61 ggtgagcgta cccgatttgc tgacgatccc gatcttcccc ttcttgaaca caaaccccgg
      121 catgatcccg accttgcatt caccggcggt gatgacaccc gggcaattgg gcccgatcag
      181 ccgcgtgttc ttacccagca ggtaattctt agccttcacc atatcctgca ccgggatacc
      241 ttccgtgata cagatgacca gggcgatacc cgcttcggca gcctccatga tcgcatcggc
      301 agcaaaggcc ggcggaacaa agatgatcga cacattggcg ccggtggtct tcaccgcatc
      361 ggctaccgta ttgaataccg gccgttccag atgcgtgctg ccacccttac ccggggtaac
      421 cccacccatt acctgcgttc catattcgat catttgtgtc gcgtgaaatg tcccttccgt
      481 ccctgtaaag ccctgtacga tgattttgga ttgcttgtta actaagattg acatgagagt
      541 agatttgata tcgtagtctt gctggtgatt cggtgggcaa aaatagggga aaatggttaa
      601 aattccccgt tatttattct gcgcatagaa cacgatcgaa gccttttcta tcgcgtttgc
      661 attgaggtaa aaaccggcca gggcgggacc cgcggtcttg tcaagcggta ttttggactt
      721 cagcgcataa acggtgacca catactcatg gatggggccg ggaggcgggc agggacccat
      781 gtacccgggc tgaccggcat cggtattaca ttggatcgcg tcggccggcg ccaggttctt
      841 cgcgatagta ccggcatcgc tcttcaggcc cgtgacattg gcggggatat tgaagatcac
      901 ccaatgccag aagccgctgc ccgtaggcgc gtccttatca taaatggtaa cggcgaaggc
      961 ctgtgttccc gccgggacat tctcccagtg aagctcgggc gagacattct ctccatgaca
     1021 gccaaaacta ttggcaaatt gtttgttcgt ggcctggccg ccgacatcgt tgcttttcag
     1081 ggtaaaggtc tgggcagaag cgccgccggc aaatacggcc agcaatacca aggagatgaa
     1141 cttttgcata ttgatgattt ttagaataca aaaatagcca ctcctccacc tcccggttag
     1201 ggccgggggc tcaaaaatta tggataaacg ttcagcggcc ggacggcacg cttctggaac
     1261 tcccggggcg tcatcccgta cacctgctta aaagactggg taaaactcga atggttctca
     1321 tatcccacct tatgatatac ctcgcttggc ttctcctggc tgtccagcat actggcggcc
     1381 agttccatcc ttcgctgcaa cagccattta ttgggggaag tggaatacag ccgggcaaat
     1441 cgtcgcttga aagtagaaag actcatattg caaaggaacg ccagctcatc cacggtgatc
     1501 gcatggttga cattcccctc gaccacagcc atcatttgcc tgtggtcggg atcatgcggg
     1561 tccgacaacc ggaaggaatg cagcagcccg ggatacttcc ccgccaggta aagcatcagt
     1621 tcctcgaact tcagcagttg caggtcctct tgtaaggccg gattggccgc cagcatcagt
     1681 tgcagcgaat cgagatagtt gcggataaaa tcatcggcgg taaacgcaac aagcgactcc
     1741 cgtcccttgc cggcatcggg cagcccggga tgccgggacc agaaatcccg gaacacactg
     1801 tcgtcaaaga aaaaaagcag gctatgatac ctgccgttga cggacagtcg ctcggtcatc
     1861 agcgtattgc cgcctgcaag cagcaggaac tgcccgccac gaatatgtat cggctcaccg
     1921 ggccggtgaa ccgtcttctc tccctccagc aaaaagctaa aaagattatt tttcaggatt
     1981 acccgcgtcc gcgaaggaga ctgcgcactg tcgtagatgc ggatggccgt tctccccggg
     2041 aaggccggag aatcgcgaac gggaaataaa tccattggta tggtgatcgt cttttccatt
     2101 cactcacttc aacaattcat ccatattccg gttgttgtcg atcttgccct ttacctccag
     2161 catccgcatg tccttggcgt cctgcaggaa ctccgaggcg aatataaaat cattcagcag
     2221 tttgttcttg ctgaagatga tctccttgtt gttgccctcc cattccttat gcccctggta
     2281 catatacatg atgtgattcc cgatctccat cacactattc atgtcgtggg tattgatcac
     2341 ggtcgtgata ttgtattcgt cggtgatctc cttgatcagc ttatcgatca gtaaggaggt
     2401 ctgggggtcg agacccgaat tgggctcgtc acaaaaaaga tacctggggt tgaggacgat
     2461 ggcccgggcg atacctaccc gcttcttcat accccccgag atctcggccg ggaatttgct
     2521 gttggcgcct tcaagattga cacgctccag cacttcgttg acgcgcttca gcttctccgc
     2581 cggggtattc ttcgtgaaca tgtccagcgg aaacatgata ttctgctcca ccgtcatact
     2641 gtcgaacagc gcggacccct ggaacagcat cccgatgttt tgccgcagct ccttccggtg
     2701 ctcctcgttc atctcggaaa aattcttgcc gtcataaacg atctctccgc tgtccggctc
     2761 gaatagcccg accaggcatt tcatcaatac cgttttgccg ctgccgctgg tgccgatgat
     2821 aagattgcat tt
//
LOCUS       DATGFG010000434         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1075994, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000434 DATGFG010000000
VERSION     DATGFG010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075994"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /locus_tag="VKQ52_08075"
     CDS             complement(<1..1688)
                     /locus_tag="VKQ52_08075"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HLZ87181.1"
                     /translation="MKTVFLSLCLGVGAPVFAQQDYPISPVLFTQVTVTDHFWLPRIE
                     TNRTVTIPASFERCKTTGRVHNFEMAALRQGKFCTVYPFDDTDIYKTIEGASYSLAVH
                     PDPALARYLDSLITIIGKAQEPDGYLYTARTIDPAHPHEWSGPERWVKEHELSHELYN
                     SGHLFEAAVAHYNATGERNLLDIALKNADLLVRVFGPDKRHVAPGHEIVEMGLVKLYR
                     VTGKKEYLDLAKFFIEMRGERQYDKTSKDEWKNGSYWQANEPVTEQEEVEGHAVRAMY
                     LYSGMADVAALTGDTTYIRAVDRLWDNMVGKKMYLHGGIGSVPDGERFGDNYELPNLT
                     TYAETCAAIGSVFWNYRMFLLHGDAKYMDVLERALYNGLISGVGLDGRSFFYTNAMQI
                     TQGMVFPAQEPARAGWFTCSCCPTNVARLLPSVPGYIYGVKGRDVYVNLFISGKAQIG
                     LGGKEKVGLVQESNYPWDGDVKIGVEVARPTAFGLLLRIPGWARGEAVPDGLYSFAGG
                     VTRPVVIRVNGQVVEARMRNGYAVIERTWKKNDKIELVLPMDVRRVVASDSLKE"
     gene            complement(1823..>2737)
                     /locus_tag="VKQ52_08080"
     CDS             complement(1823..>2737)
                     /locus_tag="VKQ52_08080"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87182.1"
                     /translation="VINSGLYTLNSNYAANFARDNKGVPETIFAIPYDHVHETGNNIN
                     MMTLNGQNQNTYNMNAQPWNGFASVQEFYASYIDTVANPGPVGKVIGTDPAGDSVRGT
                     LDKRLTNFLVGPQFGADGKRLLDNGFEPSGPGYAGDPDGAPITFTPYVNELQPTAWRQ
                     SGARISKWGFYHGMTSNLDNDFALFRYADVLLMAAEASARKNNNWSDPYALTIVNQIR
                     TVHGGVSPYTSMTATSFLAERGREMFFEGWRRQDLIRFGLYNNAWRFHPADPADNLGP
                     HGINHLNIMPLPDAQLNANQALKQNPGY"
BASE COUNT          612 a          949 c          593 g          583 t
ORIGIN      
        1 tcttccttca aactgtcact ggccaccacc cgccggacat ccatcggcaa caccagctct
       61 atcttatcgt tcttcttcca ggtgcgctcg ataacagcat acccattccg catccgcgcc
      121 tctaccacct gtccgttcac cctgatcacc accggccgcg tcacgccgcc ggcaaagcta
      181 tacagcccat ccggcaccgc ctcacccctc gcccaacccg ggatccgcaa caacaatcca
      241 aacgcggtcg gcctcgccac ctcgacccct atcttcacat ccccatccca gggatagtta
      301 ctttcctgta ccagccccac cttctctttc ccgcctaatc cgatctgcgc cttcccgctt
      361 ataaataaat tcacatacac atcccttccc ttcaccccat agatataccc cggcacactc
      421 ggcaacagcc gcgccacatt cgtcggacaa caactacagg taaaccatcc cgcccgcgcc
      481 ggctcctgtg ccgggaacac catcccctgc gtgatctgca tcgcattggt ataaaaaaag
      541 ctcctcccgt caagccccac accgctgatc agcccattgt acagggcccg ctccagcaca
      601 tccatatatt tcgcatcccc atgcaacagg aacatccggt aattccagaa gacactcccg
      661 atcgccgcac aggtctccgc atacgtcgta agattcggca actcatagtt gtccccaaac
      721 cgctccccgt ccggcacgga gccgatcccc ccatgcaggt acatcttctt gcccaccata
      781 ttgtcccaaa gcctgtccac cgcccggata tacgtagtgt cccccgtcaa agccgcaaca
      841 tcggccatcc cggaatacag gtacatcgcc cgcacggcat gcccctccac ctcctcctgc
      901 tcggtcaccg gctcattcgc ctgccagtaa ctgccgttct tccactcatc cttgctggtc
      961 ttatcatact gccgctcacc ccgcatctcg ataaagaact tcgccaggtc caggtactcc
     1021 ttcttccccg tcacccggta taatttcacc aaccccatct ccacgatctc atgccccgga
     1081 gccacatgcc gcttgtcggg cccaaacaca cgcaccagca gatcggcatt cttcaacgct
     1141 atatccaaca ggttgcgctc gcccgtggca ttgtaatgcg caacggccgc ctcgaaaaga
     1201 tgccccgaat tatatagctc atggctaagc tcatgctcct tcacccaacg ctcgggcccc
     1261 gaccactcat gcggatgcgc gggatcgatc gtcctcgccg tatacaggta cccatccggc
     1321 tcctgcgcct tgccgattat cgttatcaag gagtcgaggt aacgcgccaa cgccggatcg
     1381 ggatgcaccg ccagcgaata ggaagctcct tcaatcgtct tatagatatc cgtgtcgtca
     1441 aacggataca ccgtgcaaaa cttcccctgc cgcaaagccg ccatctcgaa attgtgaacc
     1501 cggcccgtcg tcttacaccg ttcgaaggaa gccggtatcg tcaccgtacg attggtctcg
     1561 atccgcggca accagaaatg gtcggtcacc gtcacctgcg taaaaagcac cgggcttatc
     1621 gggtaatcct gctgcgcaaa gaccggcgcc cccacaccaa gacataaaga aagaaatact
     1681 gttttcatgt tctcaattta ctccaattga agtagaccac aacaaaaaca ggcgaaagac
     1741 ttcgcccgtt tttataattg ctaaacctta gggcacgctt ttcagactcc ccatggagag
     1801 gataccggga ggcgcgccgc catcaatatc ccggattttg tttcaaagct tgattcgcat
     1861 tcagctgcgc atccggcagc ggcataatat tcaggtgatt gatcccatgt ggcccaagat
     1921 tatccgccgg gtccgccggg tggaacctcc acgcattgtt gtacaaccca aagcggatca
     1981 ggtcctgccg cctccagccc tcgaagaaca tctccctgcc ccgctcggcc aaaaagctcg
     2041 tcgccgtcat cgacgtgtag gggctcacac ccccatgcac cgtcctgatc tggtttacga
     2101 tggtcaacgc atatgggtcg ctccagttat tgttcttccg ggccgaggct tccgccgcca
     2161 tcagcaagac atccgcatag cggaataatg caaagtcatt atccaggttg gaggtcattc
     2221 catgataaaa gccccatttg ctgatccggg cgccggactg acgccaggcc gtcggctgca
     2281 gctcattgac atagggtgtg aacgtgatcg gtgccccgtc cgggtcaccg gcatatcccg
     2341 gaccactcgg ctcaaacccg ttatccaaca accgcttgcc gtcggcgcca aactgcggac
     2401 ccacaaggaa attggtcagg cgcttgtcca gcgtccccct gacagagtcg ccggcggggt
     2461 cggtaccgat gacctttccg acgggaccgg gattggcgac agtatcgata tacgaagcat
     2521 agaactcctg cacactggca aaaccattcc atggctgcgc attcatattg tatgtgttct
     2581 ggttctgccc gttcagcgtc atcatgttga tattgttgcc cgtctcatgc acatgatcat
     2641 aggggatcgc aaagatcgtc tccggcacgc ctttgttgtc cctggcaaaa ttggcggcat
     2701 agttcgaatt aagcgtatac aatcccgaat tgatcac
//
LOCUS       DATGFG010000435         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_754666, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000435 DATGFG010000000
VERSION     DATGFG010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754666"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2525
                     /locus_tag="VKQ52_08085"
     CDS             <1..>2525
                     /locus_tag="VKQ52_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ87183.1"
                     /translation="MVTDELGTPLSGASVKIKGSGAESAKGAITDRNGHFAITGPEGT
                     LVLIVTYIGYQPQELTVQAGTTVTVHLKPAQNSLDEMVIVGYGVTSRRLNTGSVSSIT
                     AADIGKETVTNPITALQGHIAGMQITQDNGLPGGGVRVNIRGAFSTVSSAGFLPLYVV
                     DGVPFTLFNGGQPATDGLNAYGVTGANGSISPFSLIDPADIDRVDVLKDADATAIYGS
                     RGANGVILITTKKGGRSKTVYSVNAYTGIEKAGHYIPMMNTAQYLQMRREAFANAGVT
                     PTAANAKDLVVWDPNAFTDWQKWAIGGTAPLTNVNASVSGGNPQNSFIFSSTYRHEGS
                     VFRGPFDATTISNRLTAGHISEDRRFSINGSVSYASMVNNLPSIDMSTIYNLAPNYPL
                     YNSDGTLNWTSTNPLSYLKQPYNATSTNLITNMTLSYKLITGLVLKTDLGYSLTRLHQ
                     TQASPASTKNPAASPISSLSYADNDNGNYIVEPQVQYNRLIGGGKLDVLGGATFQQNK
                     STGVYLYGTGFSNEALINSLTAASSVITYNNNNSLYKYNAFFGRINYSFADKYILDLT
                     GRRDGSSRFGPGHRFGNFGAVGAAWVLSQEGFMKRLPFVSFAKLRASYGLTGNDQIPN
                     YQYFPTYSAGTSTYAYQGAGISYPSNIANPDLQWETRKKGDIALELGFFHDRLSLKTD
                     FYRDRTSNLLTYISTPAQIGTTAYYGNFPALIQNQGFEFELNTVNLAGKNLRWTTAFN
                     LTLNANKLISYPGLATSSYSTTYKIGRPTDITWLYHYTGTNPATGLPTFQDLNKDGVI
                     TTADREPVKYGTPCYGGMTNDISYKGFDLNLTFQFYRRYAYTN"
BASE COUNT          596 a          896 c          589 g          444 t
ORIGIN      
        1 gcatggtcac cgatgaactg ggcacacccc tcagcggcgc ctccgtcaaa ataaaaggct
       61 ccggcgccga atccgccaaa ggcgccatca ccgaccgcaa tggccatttt gccatcaccg
      121 gcccggaagg aacactggtc ctcatcgtca cctacatcgg ctaccagccg caggagctga
      181 ccgttcaggc cggaactact gtcacggtac acctcaaacc cgcgcagaat tccctggatg
      241 aaatggtcat cgtcggctat ggcgtcacca gccggcggct gaacaccgga tcggtcagca
      301 gcatcaccgc cgccgacatc ggcaaggaaa cggtcaccaa tcccatcacc gccctgcaag
      361 gccatatcgc cggtatgcag ataacccagg acaacggcct ccccggcggc ggcgtacgcg
      421 tcaatatccg cggggccttt tccaccgtct cctccgccgg cttcctcccg ctctacgtgg
      481 tggacggagt accctttacg cttttcaatg gcggccagcc ggccaccgac ggcctgaatg
      541 cctatggcgt caccggcgcc aacggcagca tcagcccctt cagcctcatc gatccagccg
      601 atatcgaccg ggtggacgtt ctcaaggacg ccgacgccac ggccatctac ggctcccgcg
      661 gcgccaacgg ggtcatcctg atcacaacaa aaaaaggcgg ccgaagcaaa accgtctaca
      721 gcgtcaacgc ctacacaggg atagaaaagg caggccatta tatcccgatg atgaacacgg
      781 cgcagtacct ccaaatgcgc agggaagcct tcgccaatgc aggcgtcacc cccaccgccg
      841 ccaatgccaa agacctggtg gtatgggacc ccaacgcctt caccgactgg cagaaatggg
      901 ccatcggcgg caccgccccg ctcaccaatg taaatgcctc ggtctccggc ggcaacccgc
      961 agaacagttt tatcttcagc agcacttacc gccacgaagg aagcgtcttc cgcggcccct
     1021 tcgatgccac caccatctcc aaccgcctga cggccgggca tatcagcgaa gatcgccggt
     1081 tcagcatcaa cggctccgtc agctatgcga gcatggtgaa caacctgccc tccatcgaca
     1141 tgagcacgat ctataatctg gcgcccaact accccctgta taacagcgac ggcaccctca
     1201 actggacctc taccaacccg ctgtcctatc tcaaacaacc ctataacgcc acatcgacca
     1261 acctcatcac gaatatgacc ctcagctata aactgatcac cggtctcgtg ttgaaaaccg
     1321 atctgggtta ttccctcacc cgcctgcacc agacccaggc cagccccgcc agcacaaaga
     1381 accccgcggc ctcgcccatc agctccctga gctatgcgga caacgataac ggtaactata
     1441 tcgtcgagcc ccaggtacaa tacaaccggc tgatcggcgg aggcaaactg gatgtgctcg
     1501 gcggcgccac cttccagcag aacaaatcca cgggcgtcta cctctatggc acgggtttca
     1561 gcaacgaggc gctgatcaac agcctcacgg cggcaagcag cgtcatcacc tacaacaaca
     1621 acaactccct gtacaaatac aacgcctttt tcggccggat caattattcc ttcgccgata
     1681 agtacatcct cgaccttacc ggcagacggg atgggtcttc ccgcttcggc cccggacatc
     1741 gcttcggcaa tttcggcgcg gtaggggccg catgggtgct gtcccaggaa ggcttcatga
     1801 aacgcctccc cttcgtcagt ttcgctaagc tgcgcgcttc ctacgggctc accggcaacg
     1861 accagatccc caattatcaa tatttcccca cctactcggc cggcacctcc acctacgcct
     1921 accagggcgc cggcatctcc tatccctcca atatcgccaa ccccgacctg caatgggaga
     1981 cgagaaaaaa aggggacatc gcactcgaac tggggttctt tcacgaccgc ctctcgctga
     2041 agaccgattt ctaccgggat cgcacttcca atctccttac ctatatctcc actcccgcac
     2101 agatcgggac cacggcctac tatgggaatt tcccggccct gatccagaac cagggattcg
     2161 agttcgaact caatacggtg aacctcgcgg ggaagaacct tcgttggacc acggcattca
     2221 acctgacact caacgccaac aaactcatct cctatcccgg cctcgcaaca tccagctaca
     2281 gcacgaccta taagatcggc cggcctacgg acatcacctg gctctatcat tacaccggca
     2341 cgaacccggc caccggcctg cccaccttcc aggatctcaa caaggatggc gtcataacca
     2401 cggccgaccg ggaaccggtc aaatatggta cgccctgcta cggcgggatg acaaacgaca
     2461 tttcctataa aggcttcgac ctgaatctta ccttccagtt ctatcgtcgg tatgcgtata
     2521 cgaac
//
LOCUS       DATGFG010000436         4842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_404472, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000436 DATGFG010000000
VERSION     DATGFG010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404472"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..2090)
                     /locus_tag="VKQ52_08090"
     CDS             complement(162..2090)
                     /locus_tag="VKQ52_08090"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLZ87184.1"
                     /translation="MWTQTSRMAWAALLLFSLSGQAQLTEKPPLHGNHWMAVTGKPLA
                     AEAGAQIFQKGGNAIDAACAMLGAVCTMWDVLCWGGETQAVIYNPKTGKVIAIDALGW
                     APTGATPAFFKSKGYNFPPEYGPLAAVTPGTPGGLCYMLAEYGTMSLKEVLAPAMEMA
                     KGYAIEAQTANSFEAQKRRLKEWPYSKALFLPHLGEKREAPEAGEVFVQKDLLETLSK
                     MVEAEQDALKHKKTRKEAIYAACDRFYKGDIAKEFVRGCQEQGGLITLDDLAQWKPIE
                     EEPMHVNYKGVEVYKLQQWTQGPAMLQALNILENFDLKGMGYNSSRYINTVYQSMSLA
                     FADRDFYYGDPYNNEGQPMKGLLSKEYAKERAKLVNADHNDAMSAPGDPYPFEGRTNP
                     FTELLKQRAKLSTPSMRGASGASGAMNETAAAGVPANETAAVDEALYHDRLWRGTTSI
                     EAADKDGWVISVTPSGGWTPACIAGHTGVGMSQRMQSFVLDSIIDPFNVVKPHQRPRV
                     TLTPSMALKDGKPYLCFSVQGGDTQDQNLLQFFLDMVEFGMTVQEATEAPNINSDQLW
                     LSLGGEKLDDRRPRPGRILLGNVTPPYVRKELRQMGYIATYGDRTSGPINAIMIDAKH
                     GTLWGGSSNNGEDYGIGW"
     gene            complement(2071..3084)
                     /locus_tag="VKQ52_08095"
     CDS             complement(2071..3084)
                     /locus_tag="VKQ52_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HLZ87185.1"
                     /translation="MRYCFIVLLVLFCFCRCSGPAGGPVLADYFATQDSGVQTGNTRM
                     VEITTPKGKFHVWTKRFGNNPKIKLLLLHGGPGGTHEYFECFENFLPKEGIEFIYYDQ
                     LDSWYSDRPNDSSLWTTEHFVEEVEQVRQALGLNKDNFYLLGHSWGGILAMEYALKYQ
                     ANLKGLIISNMMASMPEYEKYNGVLRSKMRKSLVDSLKGYEDRGLYKDSVYQALVINE
                     YYKQHLCRLADWPDPVNRMFNHLSEGIYVTMQGPSEFKTGGRLITWDRSKDLGKIVVP
                     ALMIGGEFDTMDPRYMEWMSKQVKEGSYVYCPGGSHCDMWDDQAHYFPGLIKFLKDVD
                     ANK"
     gene            3140..4213
                     /locus_tag="VKQ52_08100"
     CDS             3140..4213
                     /locus_tag="VKQ52_08100"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLZ87186.1"
                     /translation="MKKLLHLLLLPFLITTRLFAQLPDSTEADYELKNFTFVSGETLP
                     VLRLHYTTFGKPVKDKSGKVINAVYIMHGTTGNSHNFTNPLFAGHLFNPGQLLDATKY
                     FIILPDGIGHGKSSKPSDGLHMKFPKYTYDDMVRADYTVLTQQMGINHLRLILGTSMG
                     AMHCWVWAETYPDFMDACMSNASEPIEIGGRNRISRYAAIQCVEADPAWKGGEYTEPP
                     VVGLRGAYTSLFWMTSSPWQLQRRVPTREQAERAYTTGAENFIRHQDANDMIYAFDAS
                     RLYNPEPNLEKIKCVFYAVNSADDEVNPPEMNVMEPAIKKVPKGRYILLPITEQTSGH
                     GTHTNPTVWGNYLKELLSASEKK"
     gene            complement(4225..>4842)
                     /locus_tag="VKQ52_08105"
     CDS             complement(4225..>4842)
                     /locus_tag="VKQ52_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87187.1"
                     /translation="VKENFVAVPGRGFLLMKEYGRAYEQDLDEATFDEGWDPAFLFAS
                     NDIPDPSAGPSPMRMRLPEPRYGYARYRETSSPVYHDRGDLSLYYFPASRKDSCWSGM
                     ISQEQVTELNSPNLSYMMVPMQDRIVFLYNSFVHGEKMYASTTVIDPRGEQVTNEGIL
                     FWGLKNTLDFQQARQVAPDQVVIPYDIYVNGNANGKIGFAVVLFR"
BASE COUNT         1151 a         1707 c          981 g         1003 t
ORIGIN      
        1 cacccgttga attctacacc tccgatctgc ccggcgacta tctcatcgtc atccagggcc
       61 tctcccccga tggccgcgcc ggcgtcacat acaaacgctt caccgtaaaa cccggccacc
      121 cctgaccctc cgacccaaca acccaagccc gcccgttaat cctaccatcc tatcccataa
      181 tcctccccgt tattactact cccaccccaa agggtcccat gcttcgcatc gatcatgatc
      241 gcatttatcg gcccgctcgt ccggtcccca taagtggcga tataccccat ctgccgcaac
      301 tccttccgca catacggcgg cgtcacattc ccaagcagga tccggcccgg ccggggacgc
      361 cggtcatcca gcttctcccc gccaagcgac aaccacaact gatcggaatt gatattcggc
      421 gcctccgtcg cctcctgcac cgtcatcccg aactccacca tatcgaggaa gaactgcaat
      481 agattctgat cctgcgtatc cccaccctgt acgctaaaac agagataggg cttgccatcc
      541 ttcaacgcca tactgggcgt caacgtcacc cgcggccgct gatgcggctt gaccacatta
      601 aacggatcga tgatggaatc cagcacaaaa ctctgcatcc gctgactcat ccccacaccc
      661 gtatgccccg cgatacaagc cggggtccat cccccgctcg gcgtgacgga aataacccag
      721 ccgtccttat ccgcagcctc gatactcgtc gtcccacgcc acaaccggtc atgatacaac
      781 gcctcatcca ctgccgccgt ctcattcgcc ggaacaccag ccgccgccgt ctcattcatc
      841 gccccgctcg cacccgacgc cccccgcatc gaaggagtgc tcagcttcgc ccgctgcttc
      901 aacaactcgg taaaaggatt cgtccgtccc tcgaaaggat aaggatcacc cggcgccgac
      961 atcgcatcat tgtgatcggc attgaccagc ttcgcccgct ccttcgcata ctccttgctc
     1021 agcagaccct tcatcggctg cccctcatta ttatatggat cgccataata aaaatcccgg
     1081 tccgcaaaag ccagactcat cgattgatat accgtattga tataccggct gctgttatac
     1141 cccatcccct tcagatcgaa attctccaaa atattcaaag cctgcagcat cgccggcccc
     1201 tgtgtccact gctgtagctt gtacacctcg acgcccttat aattgacatg catcggctcc
     1261 tcctcgatcg gcttccactg agccaggtca tcgagggtga tcaacccacc ctgctcctgg
     1321 caaccccgta caaactcttt cgcgatatcc cctttgtaaa aacggtcaca agccgcataa
     1381 atggcctcct tccgcgtctt cttatgcttc aacgcatcct gctccgcctc caccatcttc
     1441 gaaagcgtct cgagcaggtc cttctgcaca aagacctccc ccgcttccgg cgcctcgcgc
     1501 ttttccccca ggtggggaag gaacaacgcc ttgctgtaag gccattcctt cagccgccgc
     1561 ttctgcgcct cgaaactatt cgccgtctgc gcctctatag catagccctt cgccatctcc
     1621 atcgcgggtg ccaacacctc cttcagactc atcgtcccat attccgccag catataacaa
     1681 agcccacccg gcgtccccgg cgtaaccgcc gccaacggac catattccgg cgggaaatta
     1741 tatcccttgc tcttgaaaaa ggccggcgtc gccccggtcg gcgcccaccc taatgcgtcg
     1801 atggcgatca ccttcccggt cttcggatta tagatcaccg cctgcgtctc cccaccccag
     1861 caaagcacat cccacatcgt acacaccgcc ccaagcatcg cacaggccgc atcgatcgca
     1921 ttgccaccct tctggaagat ctgcgccccc gcctccgcag ccaacggctt gcccgtcact
     1981 gccatccagt gattgccatg caacggcggc ttctcggtaa gctgggcctg cccggataaa
     2041 gaaaagagca gcaacgccgc ccacgccatc ctacttgttt gcgtccacat cttttaaaaa
     2101 ttttataagt cctggaaaat aatgcgcttg gtcatcccac atatcacaat gactcccccc
     2161 gggacaatac acataactcc cctccttcac ctgcttgctc atccactcca tatacctggg
     2221 gtccatcgta tcgaactccc cgccgatcat caaagccggc actacgatct tccccaggtc
     2281 cttgctcctg tcccaggtga tcagtctccc acccgtcttg aactcacttg gcccctgcat
     2341 cgtcacatag ataccctcgc tcaaatggtt gaacatccgg ttcaccgggt caggccagtc
     2401 cgccagccga cacaaatgct gcttgtaata ttcattgatg acaagcgcct ggtagacact
     2461 atccttataa agccccctgt cctcgtaccc cttcagcgag tcgacaagcg actttcgcat
     2521 cttcgaccgt agaacaccgt tatacttctc atactccggc atgctggcca tcatattcga
     2581 aatgatcaac cccttcagat tggcctggta tttcaacgca tattccattg caaggatgcc
     2641 accccagcta tgacccaaca ggtaaaaatt atccttgttc aatcccagcg cctgccgcac
     2701 ctgctccacc tcctccacaa aatgctccgt cgtccacaaa ctactgtcat tcggccggtc
     2761 gctataccag gaatccagtt gatcataata gatgaattcg atcccctcct tcggcagaaa
     2821 attctcgaaa cactcgaaat attcatgcgt tccccccggc ccgccatgca gcaacagcaa
     2881 tttgatcttc gggttattcc cgaaccgctt ggtccatacg tggaatttcc cctttggcgt
     2941 ggtgatctcc accatcctgg tattaccggt ctggaccccg gaatcctgcg tggcgaaata
     3001 atcggccagg accggccccc ccgccggccc cgaacaacgg caaaagcaaa acaataccag
     3061 caagacaata aagcaatatc tcataagact cattttttac gtagtaacat caatataata
     3121 aaattcctac attcactgga tgaaaaagct cttacatctt ttacttctac cctttctcat
     3181 caccacccgc ctgttcgccc agctccccga ctccaccgaa gcggattacg agctgaagaa
     3241 cttcaccttc gtcagcggcg agacccttcc tgtactccgg ctgcactata ccaccttcgg
     3301 caaacccgta aaagacaaga gcggaaaagt aataaacgcc gtctatatca tgcacggtac
     3361 aacgggcaac agccacaatt tcaccaaccc gctcttcgcc ggccacctct tcaatcccgg
     3421 acagttgctc gacgccacaa aatattttat catcctgccc gacggtatcg gtcacggcaa
     3481 gtccagcaaa cccagcgacg gtctgcacat gaaattcccg aaatatacct atgatgacat
     3541 ggtcagggcg gattacaccg tcctcaccca acagatgggc atcaatcacc tccgcctcat
     3601 cctgggaacc tccatggggg ccatgcactg ctgggtctgg gccgagacct accccgattt
     3661 catggacgcc tgcatgtcca acgcaagcga acccatcgag atcggcggcc gtaaccgcat
     3721 ctcccgctac gcggcgatcc aatgcgtcga agcagacccg gcctggaaag gcggggaata
     3781 tacggaacca ccggtggtcg gcctgcgcgg cgcctatacc tccctgttct ggatgaccag
     3841 cagcccctgg caactgcaac gccgcgttcc cacccgcgaa caggctgaaa gggcctatac
     3901 cacaggcgcg gagaacttca tccgccacca ggacgcaaat gacatgatct acgccttcga
     3961 cgcctcccgc ctctataacc ccgaaccaaa cctggaaaaa ataaaatgcg tcttctacgc
     4021 cgtcaattcc gcggacgatg aggtcaaccc gcccgaaatg aacgtcatgg aacccgccat
     4081 aaaaaaggtc ccgaaaggaa gatacatcct cctgcccatc accgaacaga catccggtca
     4141 cggcacccat accaacccca ccgtctgggg taattacctg aaagaattgc tgtcggcaag
     4201 cgaaaaaaaa taactatcgg aaaactatcg gaaaagcacc accgcaaacc cgatcttccc
     4261 attggcattc ccattgacgt aaatgtcata cgggatcacc acctggtccg gcgccacctg
     4321 cctcgcctgc tggaagtcaa gcgtattctt caacccccag aaaagaatgc cctcattggt
     4381 cacctgctca ccccgcggat cgatcacggt cgtggaagca tacatcttct ccccgtgtac
     4441 aaaactgttg taaagaaaaa cgatccggtc ctgcatcggc accatcatat aggaaagatt
     4501 gggggaattc agttcggtca cctgctcctg gctgatcatc ccgctccagc aactgtcctt
     4561 ccggctcgca ggaaaataat ataggctcag atcgccacga tcatgataaa ccgggctcga
     4621 tgtctcccgg tatcgcgcat acccataccg cggctccggt aaccgcatcc gcatgggcga
     4681 aggccccgca ctcggatcgg gaatatcatt cgaagcaaaa aggaacgcgg ggtcccaccc
     4741 ctcatcgaat gtcgcctcat ccagatcctg ttcatacgcc cggccatatt ccttcatcag
     4801 gagaaaaccc ctccccggta cggccacaaa attctccttc ac
//
LOCUS       DATGFG010000437         6360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1524033, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000437 DATGFG010000000
VERSION     DATGFG010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524033"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VKQ52_08110"
     CDS             complement(<1..865)
                     /locus_tag="VKQ52_08110"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLZ87188.1"
                     /translation="MQVTQIDYLHEVGAKKNQEDYLWPLPGTASSQDKVFIVCDGVGG
                     SESGEVASKLVAESVGEALLKTPLSGVGLPLINELLEAARLKLVEYATLKGLSTDMAT
                     TFTLLHLVKERAFIAWCGDSRVYHLRKNEILFRTDDHSLVHSLIRKGEMTEEEARNHP
                     QKNLLLKAIRADDSRPEAEGHWIEDIQEGDYFLLCTDGLLENISDADLLFLLHQNELG
                     EIDLVKAFRQYCYDKTRDNYSMYLIRVSPGPVEEEEKRPAGKGIRRRVGWLLFLLLIL
                     VAAAAFIIKENY"
     gene            complement(868..3738)
                     /locus_tag="VKQ52_08115"
     CDS             complement(868..3738)
                     /locus_tag="VKQ52_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ87189.1"
                     /translation="MKDLSTVLLLFAGLLTVERTVAQDSLSNRDAADIRYKAERLVKT
                     DLNELLNSISNTGFESQEVAESIHSSYSESRTRIFRDSLVVVEPDLNPVFQNSAQSGD
                     ALLDKYLKDIDILYKKSDSATIEFSNIRCSSVKKKDQFYIKVYYNCLFRGKSSVADQP
                     YSVTNRIAEIQAERQKNTWRLLIVRIGYFNPIDTANDVANNVPIKYDMKLTGLTAQST
                     AQDSAAAIQKQISFEEEEQGRIARQQEEQDRLEEEKFKKLMSLGNIAYRQKDYTAAMQ
                     HYKDAQEIRPNSPEVNLAMKTTNNAMTQFKYESARLYQDFLNKALLQAQNRQYKEAIE
                     SYQNAVKQKPEEAATLEPKIRELTDKFAILSDLQEKYNAGFIKEALKEYTDAIKKDPK
                     NSDYYLGRGRCHEKMTDESKSTANALKDYTQAYELDHQNLAAVRCRAELYARTGDYFK
                     ALSDYKICLTLDKENMEFYERKSEMHVLLKLPADAISDLDEALKIDPKASHVYLVKGL
                     LQYDLKETGKASENFTTCLRIDSSNALAWFNRGRCDILQNKIQAAATDFGSARDKGLD
                     SNNTRIVNEYARKYYERAVAQFSNNRTDSAVIFIDYAISIDLTSALYRFTRGDYYYSL
                     GRYNEAIGSYDRAIQYNPSYMAAFYKRGMARYGIADYKESIVNFEAALKLDPSNLLAE
                     KGDGDAYQALKDYANASIAYENTLRIAANVKPAPSPTLLAEVYNSLGHCYFEQADYEK
                     AVNNGKKALSSNRNFAEAYFNRGYAYYKQGQLSDAIDDLTKANSFESNHLQWHYVLAR
                     AYQDKKDLANAAVQYSSCVQKDSALSFPDAIYKQAYCNYMMQNYTGALPLYSRSLALH
                     LDTAVAGFNIEMGVVYLNMGKYDSAYYFCQKAYRKDSTNGYASYGIGSSLALQGKADE
                     SITWFERSFQTKTPSYGEIKKDKLLGDMRNNKKFKELLKKYY"
     gene            complement(3785..4654)
                     /locus_tag="VKQ52_08120"
     CDS             complement(3785..4654)
                     /locus_tag="VKQ52_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87190.1"
                     /translation="MIYSPWKAICCLSVSVLLCRLSSPAQVFSNDDGVREANFVTKVV
                     QLDEFIHRFNNDPNSGIRQYYLSHHRPFDKSRAQLIRSLFNYSQNWDSVQMDRFVQRA
                     TDPQRPDSLGFLQGKWYAEAKCSFLYNAAPVEADLILRIQLNPDGTAQWVIAAVKPDF
                     ALAREAAAAPVIPLSPAQKRVRFIQPAANDTYFAELDRDFADKRNLPAFFDQQFFERR
                     NSGPFYEALLKDRIRFVAVKKLRYHYLQVHDWIFTVENYERNTRNSGWLISSIRAAGK
                     EERADYEKKLLDE"
     gene            complement(4670..5881)
                     /locus_tag="VKQ52_08125"
     CDS             complement(4670..5881)
                     /locus_tag="VKQ52_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87191.1"
                     /translation="MNCLTSLTAAWMCLLCSNLLHAQDRICLENEMRQGKVNDISLST
                     VRYTPADRPNQPMLLPLNRILILFNSDGGYLVPSQMDSSNPNIRAYLNRFFSPPVHTR
                     TRDQVFTVQHTLLEVKIAREDDEYVYWEDNGRINKKDIAAVIYSNGTPKVLCSMSQAA
                     LILGTCQRTPLNLGIGTDLIISNEPVGANGDAGTKTGGAANGSGSTDVAPVLPAGGSS
                     TAGGDSSDSINRAKVKALLGNVKPEEFTDKAQKKTVELTGYLKILCDKTATTEDLDKA
                     VAQALALFISDSAVVEVSSNNRNAITRRPIREYLRHLKQIQYDKIEVKWTHVQYVSEL
                     KPGPDGRLYGTVSFEQEFKGYKDGKLVYSDVTIKHANVVLVTYQKITDGSAQQLWDVR
                     LGDIGVFAQKT"
     gene            complement(6115..>6360)
                     /locus_tag="VKQ52_08130"
     CDS             complement(6115..>6360)
                     /locus_tag="VKQ52_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87192.1"
                     /translation="GSAWSSARYVLTLATARTCAIHINDIPYGVLEGGKTMRVYLVPG
                     DYTIQASTIGNHPEVYTRRLVVTRQNLNHWRRFTIGL"
BASE COUNT         1366 a         1833 c         1619 g         1542 t
ORIGIN      
        1 aataattctc cttgatgatg aaggctgctg cagccaccag gatcagcagc aggaacaaca
       61 gccagccgac ccgtcgccgg atccccttcc cggccggcct cttctcctcc tcttcaacag
      121 gccccggact cacccggatc aggtacatcg aatagttatc cctcgtctta tcataacaat
      181 actgccggaa agccttcacc aggtcgatct ctccaagctc attctgatgc agcaggaaca
      241 acaggtcggc atcggatata ttctccagca gtccgtccgt acaaagcagg aaataatccc
      301 cttcctgtat atcctcgatc caatgcccct ccgcctccgg ccgcgaatca tcggcgcgga
      361 tagccttcag caacaggttc ttctgcggat gattccgagc ctcctcctcc gtcatctccc
      421 ccttccggat aagcgaatgg accagggagt gatcatccgt ccggaaaagg atctcgttct
      481 tgcggagatg gtatacccgg ctgtccccgc accaggcgat gaaggcccgc tccttcacca
      541 aatgcagcag cgtaaaggtc gtggccatat ccgtgctgag ccccttcagg gtggcatact
      601 ccaccagctt caaccgcgcc gcctcgagca attcgttgat aagcggcagc cccacgcccg
      661 aaagcggagt tttcaaaagc gcttcaccca ccgactccgc caccagcttg ctggccacct
      721 ccccgctctc cgatccccct acaccgtcgc aaacgataaa gaccttgtcc tgcgacgaag
      781 ccgttccggg cagtggccag agatagtctt cctggttctt cttcgcgccg acctcgtgga
      841 gatagtcaat ttgcgtaacc tgcattatca ataatatttt ttcaggagtt ccttgaattt
      901 tttattgttg cgcatgtctc cgaggagctt gtcttttttt atctcgccat agctgggtgt
      961 ttttgtctga aaggatctct cgaaccaggt gatcgactcg tccgccttcc cctgcagggc
     1021 aagcgaggag ccgatgccat aggaagcata accattggta ctgtccttcc tgtaagcctt
     1081 ctgacagaaa taataggcgg aatcatactt gcccatgttc aggtaaacga cccccatctc
     1141 gatattgaaa ccggctacag ccgtatccag gtgtagcgcc agactgcggg aatacaaggg
     1201 cagcgccccc gtatagttct gcatcatata gttgcagtag gcttgtttgt agatggcgtc
     1261 ggggaaagaa agcgccgaat ccttctgtac acaggagcta tactgcaccg cggcattagc
     1321 aaggtctttc ttatcctggt aagccctcgc cagcacataa tgccattgaa ggtggttgct
     1381 ttcgaaggag ttggccttcg tcagatcatc tatcgcatcc gacaactgcc cctgcttgta
     1441 gtaggcatat cccctgttga aataggcctc ggcaaaattg cggttactgc taagcgcctt
     1501 cttcccatta tttaccgcct tttcataatc ggcctgctcg aaataacaat gcccaaggga
     1561 attgtacacc tccgccagca gggtagggct gggtgcgggc ttcacattgg cggcgatacg
     1621 cagggtattc tcataggcta tcgacgcatt ggcataatcc ttcaatgcct ggtaggcatc
     1681 cccatcgcct ttctcggcaa gcaggttcga cggatcgagc ttcagcgctg cctcgaaatt
     1741 gacgatcgat tccttgtaat cggcaatccc atagcgcgcc atcccgcgtt tgtaaaaggc
     1801 cgccatataa gaagggttat actggatggc cctgtcataa ctcccgatcg cttcgttata
     1861 cctgccaagc gaataataat agtcaccccg ggtaaaccgg tataaggccg aagtcagatc
     1921 tatcgaaata gcataatcga taaagatcac cgcggaatcc gtccggttat tgctgaactg
     1981 tgcaaccgcc cgctcataat atttccgggc atactcgttg acgatccggg tattgttgct
     2041 gtccaacccc ttgtcccgcg cgctgccgaa atccgtggcg gcggcctgga tcttgttctg
     2101 caggatatcg caacgccccc ggttgaacca ggccagcgca ttggagctat cgatccgcag
     2161 acaggtggtg aaattctcac tggccttgcc ggtctccttc agatcgtatt gcaggaggcc
     2221 cttgacaagg tagacatgcg aggccttggg atcgatctta agcgcctcgt cgagatcgct
     2281 tatcgcatcg gcgggcaatt tcagcaacac atgcatctcc gacttccgct catagaattc
     2341 catattctcc ttgtccaggg tgagacagat cttgtagtcc gacagggcct tgaaataatc
     2401 ccccgtccgg gcatacaact cggcccggca acgaacggcc gccaggttct ggtggtccag
     2461 ctcatatgcc tgcgtgtaat ccttcagcgc attcgccgtc gatttcgatt catcggtcat
     2521 cttctcgtga cagcgcccgc ggcccaaata ataatcggag tttttggggt ccttcttgat
     2581 ggcgtccgta tactccttca atgcctcctt tataaaaccg gcattatact tctcctgcag
     2641 atcggaaaga atggcgaact tatccgtcaa ctcccggatc ttgggttcca gggtggcggc
     2701 ttcctccggc ttttgcttca cggcattctg gtaactctcg atagcctcct tgtattgccg
     2761 gttctgcgcc tgcagaagcg ccttgttcaa aaaatcctgg tagagcctgg ccgactcata
     2821 tttgaactgc gtcatcgcat tgttcgtcgt cttcatcgcc aggttcacct cgggggagtt
     2881 gggcctgatc tcctgcgcat ccttgtaatg ctgcatcgcc gcggtataat ccttttgccg
     2941 ataggcgata ttgccaagcg acatcagctt cttgaacttc tcttcttcca gcctgtcctg
     3001 ctcctcctgc tgcctggcga tgcggccctg ctcttcctcc tcgaagctga tctgcttctg
     3061 gatcgctgcc gcggagtcct gggccgtgct ctgcgcggtc agcccggtga gcttcatatc
     3121 gtatttgatc gggacattgt tggccacatc attggccgta tcgatgggat tgaaataccc
     3181 gatgcggacg atcagcagcc gccaggtgtt cttctgccgc tcggcctgga tctccgcgat
     3241 cctgttcgtc accgaatacg gctgatcggc caccgaactc tttcccctga acaggcagtt
     3301 atagtacacc ttgatgtaga actggtcctt tttctttacg ctgctgcacc ggatattgct
     3361 gaactcgatg gtggcgctat cgctcttttt ataaaggatg tcgatgtcct tgagatactt
     3421 gtcaagtaag gcatcccccg actgcgcgga attctgaaac acagggttca gatcgggttc
     3481 caccaccacg agcgaatccc ggaagatgcg ggtccggctc tccgaatagg aggaatggat
     3541 gctctcggcc acttcctgtg actcgaaacc cgtgttcgaa atggagttca gcaattcatt
     3601 aaggtcggtc ttcaccaatc tctcagcctt gtagcggata tcggctgcat ctcggttcga
     3661 aagggagtcc tgggcaaccg ttcgttcaac ggtaagaagg ccggcaaaaa gcaacaggac
     3721 ggtggacaaa tctttcataa ctagggttta taaagtggtt tttatggcgt gcctggtcgg
     3781 tacatcattc atccagcagc ttcttctcat aatcggctcg ttcttccttg ccggcggcac
     3841 ggatgctgga tatcagccag ccggaattgc gggtattccg ctcataattt tcgaccgtaa
     3901 atatccagtc atgcacctgc agataatgat acctgagttt ctttacggcg acaaaccgga
     3961 tcctgtcttt taacaacgcc tcgtagaagg gtccggaatt ccggcgttcg aagaactgct
     4021 ggtcaaagaa agcggggaga ttgcgcttgt cggcaaaatc ccggtccagc tcggcaaaat
     4081 aggtatcgtt ggccgccggc tggatgaacc tgacccgctt ctgcgcgggc gacagcggga
     4141 tcaccggcgc cgccgccgct tcccgggcaa gggcgaaatc cggtttaacg gccgcgatca
     4201 cccattgtgc ggttccatcg gggttcaatt gtatccgcag gatcaggtcg gcttctactg
     4261 gcgcggcatt gtacaggaaa ctgcactttg cctcggcgta ccacttgccc tgcaaaaacc
     4321 ccagcgaatc cggccgctgc ggatcggtgg cccgttggac gaaacggtcc atctgcaccg
     4381 aatcccagtt ctgggaatag ttgaaaaggc tccggatcaa ttgcgcccgg ctcttatcga
     4441 acgggcggtg atgcgaaaga tagtattgcc gtatccccga attcggatca ttgttgaacc
     4501 ggtgaataaa ttcatccagt tgtaccacct tcgtcacaaa attcgcttcc cggacaccat
     4561 cgtcattact gaagacctgt gcgggggaag acagtcggca taagaggacg gatacagaca
     4621 aacaacaaat agccttccat ggggaatata tcatgtttct gatgggttgt catgttttct
     4681 gggcaaatac tccgatatcc ccaagacgca catcccaaag ctgctgggcg cttccgtcgg
     4741 tgatcttctg gtaggtgacg agtacgacat tggcgtgctt gatggtcaca tcgctataga
     4801 ccagctttcc atccttatag cctttgaact cctgctcgaa agacaccgta ccgtacaacc
     4861 tcccgtccgg cccgggcttc agctcgctca catactggac atgcgtccac ttcacctcga
     4921 tcttatcgta ttgtatctgc ttcaggtgac ggagatattc tcttatcggt cgccgggtga
     4981 tggcattccg gttgttggaa gacacctcca ccaccgcgct gtcgctgatg aacagggcga
     5041 gcgcctgcgc cacggccttg tcgagatctt ccgtggtggc cgtcttgtcg caaaggatct
     5101 tcagatagcc ggtgagctcc accgtcttct tctgagcttt gtcggtgaac tcttccggct
     5161 tcacattccc gagcagggcc ttcaccttgg cccggttgat gctgtcggag ctgtcgccac
     5221 ccgcggtgga cgaaccgcct gccggtaata cgggcgccac atccgtgctg ccgctgccgt
     5281 tcgcggcgcc gccggtcttc gtaccggcat ccccgttagc acccaccggc tcgttgctga
     5341 tgatcaggtc ggtcccgatc cccaggttga gcggcgtccg ctgacaggtt cccaggatca
     5401 gcgcggcctg gctcatggaa cacaacacct tcggcgtccc gttcgaatag atcacggcgg
     5461 caatatcttt cttatttatc cgcccgttat cctcccagta gacatactca tcatcctccc
     5521 gggcgatctt cacctccaga agcgtatgct ggaccgtaaa gacctggtcc ctggtgcgcg
     5581 tatgcacagg tggtgaaaag aaccggttga gataggcgcg gatattcgga ttggagctat
     5641 ccatctggga aggaacaaga tagccgccat cggaattgaa caggatcagg atcctgttca
     5701 ggggaagcag catgggctga ttgggcctgt ccgcgggagt atagcgaacg gtgctgaggg
     5761 aaatatcatt caccttcccc tgacgcatct cattttccag acagatccta tcctgcgcat
     5821 gaagaagatt cgaacagagc aagcacatcc aggctgctgt cagcgacgtt aagcagttca
     5881 taatgaaaac agaatttgga atgatcaggt acggcgcaaa agggatacga acggccggac
     5941 tggaaccttc acggatagga atttttcttc gacagaggag cagaaagata ctaggaaaag
     6001 ctgagatcgc taagcttttc gccacaattt attctaccat aatagtggta ttgcggcctg
     6061 tggtaagtgg gtagtttctc ctctgaaacc accggcagcc agggcggctg cggcttacag
     6121 cccgatagta aatctccgcc agtgattcag gttctgccgg gtgacaacca gcctgcgggt
     6181 gtaaacctcc ggatgattgc cgatggtact cgcctggata gtataatctc ccggtacaag
     6241 atacacccgc atggtcttgc cgccctccag caccccatag gggatatcat tgatatgaat
     6301 ggcacaggtc ctggctgtgg ccagcgtcag gacgtacctt gctgacgacc acgccgatcc
//
LOCUS       DATGFG010000438         5578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1616347, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000438 DATGFG010000000
VERSION     DATGFG010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616347"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VKQ52_08135"
     CDS             complement(<1..186)
                     /locus_tag="VKQ52_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87193.1"
                     /translation="MARIGRKILSAFIDVGEDKKEDVPAGKPAAGNSAGAGNSAGSGN
                     SVGLGTAAGSGTGAGAGR"
     gene            complement(230..391)
                     /locus_tag="VKQ52_08140"
     CDS             complement(230..391)
                     /locus_tag="VKQ52_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87194.1"
                     /translation="MKKLLGVAIILLTLILSVIYRQLSHRPVKENPQHAYVPATLRDG
                     MLLSYLLFQ"
     gene            783..1637
                     /locus_tag="VKQ52_08145"
     CDS             783..1637
                     /locus_tag="VKQ52_08145"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ87195.1"
                     /translation="MRRLPILLFIVSSVFAHAQDTARQIPLWPKGAPGFENKRNEPEQ
                     ARDYWVKNIHNPSITTYLPPKEKATGAAVVIFPGGGHRLLVFNAEGRDPARFLNSLGI
                     AAFIVKYRLFREDSTLYSLDREVKQDAYRAMRLVRSKAAEWHIDPDRVGTLSFSAGGE
                     VANIIAYGPGKGDPQAPDPIDRLDGKPNFQLLVYPGPLGVPETVPADAPPAFLIAAND
                     DPCCSTPVVTILEKYRTAHVPVEAHILSGGSHGFNMGYRSNLAAVKAWPQLMANWLTD
                     QGLTKPKK"
     gene            complement(1994..2578)
                     /locus_tag="VKQ52_08150"
     CDS             complement(1994..2578)
                     /locus_tag="VKQ52_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87196.1"
                     /translation="MKILKLLSGIILFLTATVSQAQPDTAKITQRALLYADSLVKADA
                     FENWSLYADLAPFSVTKYYGGKDGYIGHVQMVRTFTISAQQEAAPELQMQTLVTLKDQ
                     WQCVIRLSRYFHRDGTKYHLVSYLIGQSKDEGENWKIFDVNYNTVGNLMQIFPDILDM
                     PIKETLVLSEEQELAQQAAAAAANPAKKSGAKRK"
     gene            2764..3825
                     /locus_tag="VKQ52_08155"
     CDS             2764..3825
                     /locus_tag="VKQ52_08155"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLZ87197.1"
                     /translation="MSQRSDFVYSAKQEPHRLRTKQILQQHPEVRKLIGKNKYTIFAV
                     LGLVLFQVVLAWVVKDRSWWMVVGAAYLLGAFADHSLFVMIHECAHHLLFKNRAANRL
                     AGILANLPQLFPSSVSFERYHIKHHSFQGVHELDADLPNRWEAKLINNYFIGKVIWLL
                     FYPLFQVFRISRLREIKPFDKWVALNWTAEVLFVAAIWYFLGPKAIVYLLLSFFFSVG
                     LHPLGARWIQEHYLTHDEQQETFSYYGVLNTVAFNVGYHNEHHDFPSIPWNKLPQIKK
                     TAPDYYDSLVSHKSWTVLFFRFLFDKEISLYSRIVRKERGKVALTDESRPDMDLVSAT
                     QTAPGPAHHAPGAAVITQA"
     gene            complement(3884..4762)
                     /locus_tag="VKQ52_08160"
     CDS             complement(3884..4762)
                     /locus_tag="VKQ52_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ87198.1"
                     /translation="MSTNIIREITPLTQNDCFTIFSRVKKEFSFPLHYHEELELNLII
                     NAKGARRIVGDHIDTIEDLELVLVGPNLYHAWFTHQCKSEEIREVTIQWHKDLFEEKL
                     LRRNQLSFIRNMMDRSQKGILFSRETATRLAPRIMTLNQKNGFDSVLELLSILHDLSI
                     SRNMRTLSDASFTNQHFTYNSRRIEKAFEYMNNNYDKPITLGEVARLVSMTEVSFSRF
                     IKKRTGNTFIDSLNEIRLGHASRMLIDTTHSISEISYHCGFNNISNFNRIFKKKKTCT
                     PKEFRENFNSGTRVFI"
     gene            5041..>5578
                     /locus_tag="VKQ52_08165"
     CDS             5041..>5578
                     /locus_tag="VKQ52_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87199.1"
                     /translation="MKLQLPDFAILIAYLVTMVVIGWNLRKKARQNKLNYLLGGKSLP
                     WYMLGLSDASDMFDISGTMWMVSLCFVYGMKSIWIPWLWPVFNQVFLMVYLSRWLRRS
                     NATTGAEWLATRFGRSGPGVRASHNIVVAFALLSCFGFMAYGFVGLGKFMQIFIPWDS
                     IKGSLPFHIPPAYVPHFYG"
BASE COUNT         1360 a         1673 c         1251 g         1294 t
ORIGIN      
        1 acgaccggcc cccgccccag tccccgaccc cgccgcagtc cccaacccca cggaattccc
       61 tgaccccgcg gaattcccgg ctcctgccga attccccgcc gccggcttac cggccggcac
      121 gtcttctttt ttatcctccc cgacatcgat aaatgcggaa aggatcttcc tgccaattct
      181 ggccatagtg attgttttaa atggttaaaa aatctttatt tccgggctgc tattggaaga
      241 gcaggtacga cagcagcatc ccatcccgca gcgtagcagg cacatacgcg tgttgcgggt
      301 tctccttcac cggccggtga gacaattgcc ggtagatcac ggataagata agagtgagca
      361 aaatgatagc cactccgaga agctttttca tattcggtta gttaggacag taatgttata
      421 acgggaatct gtattcgccg gaaggcgatg ggagaatggg ttttcggaac gtcaacccaa
      481 tggagaatgt ttcataattg taggagacaa ggctccgttt gatttgatgg agtaaagata
      541 gtagtgggtt gcccctccct gatagtacat ccggtaggag cggtcccttt tgtttaatga
      601 cggtccgcct cctgtgataa gcggtaaaat aggtgggtga gtgtcaaata tcaggtctga
      661 tcggtaattt tcgagagtcg agcggctgcc ggccagtaag agtggtaacc gctctttacc
      721 tatcaccgtt aggttgctac gccgttgcaa atccgtatct tcaggaaccc aaagatcaat
      781 ttatgcgtcg ccttcccatc ctccttttca tcgtctcctc cgtatttgcc catgcccagg
      841 acaccgcccg gcagatcccg ctctggccaa aaggcgcacc aggcttcgag aacaagcgca
      901 acgaacccga acaggcccgg gattactggg tgaagaacat ccacaaccca agcatcacca
      961 catacctgcc gccaaaagaa aaagcgacag gcgcagccgt tgtcatcttc cccggcgggg
     1021 gccacaggct gctggtattt aacgccgaag gaagagaccc cgcccgtttc ctgaacagcc
     1081 tgggtatcgc agcctttatc gtgaagtatc gtttattcag ggaagattca accctctata
     1141 gcctcgacag agaagtaaaa caggacgcct accgggccat gcgccttgtg cgcagtaaag
     1201 ccgccgaatg gcatatcgac cccgaccggg tcggaaccct gagcttttcc gccggcggcg
     1261 aagtcgccaa tatcatcgcc tatggcccgg gtaaaggcga cccgcaggcc ccggacccca
     1321 tcgaccgcct cgacggcaaa ccgaactttc aattgctcgt ctatcccgga ccgcttggag
     1381 tacccgaaac cgtacccgcc gacgcacccc cggccttcct catcgccgcc aatgacgacc
     1441 cctgctgctc aacacccgtc gtcaccatcc tcgaaaaata ccgcaccgcc cacgttccag
     1501 tggaagcgca tatcctatcc ggtggcagcc acggctttaa tatgggctac cgctccaatt
     1561 tagcagccgt aaaagcctgg ccacagctaa tggccaactg gctcaccgac cagggactca
     1621 caaaacccaa aaaataaact gcccgcctcg ggcaaaagaa tagcgcctgc ccaaaccgga
     1681 atgggcgcag gcccaaaaaa aaagcccccg gtaagaatac caggggcgta ctaatcaaag
     1741 tgtttttaac cattaaacct tatcctatga aaaaccaaat gtacacccgt aatctggcaa
     1801 ttccctgaat ctggtctatg gttattaata ctttaggaac ttactttaaa aatatcttgg
     1861 taaaccactt aaaaattgat cttggcgcca ggcgacttca tccgcaaaaa gcaggttttc
     1921 atcccgcacg ggccgcccgc tgacccgaaa accgatccca ccaccgagcc ggccacccac
     1981 ccgcgccccg gccctatttc ctctttgccc cgctcttttt agccggatta gccgccgcgg
     2041 ccgccgcctg ctgcgccagc tcctgttcct cactcaaaac caacgtctcc tttatcggca
     2101 tatcaaggat atcgggaaag atctgcatca aattccccac cgtattataa ttgacgtcaa
     2161 agatcttcca gttttcccct tcatctttgg actgaccgat caggtaagag actaagtggt
     2221 attttgtacc atccctgtgg aaataccggg atagccggat cacacactgc cactggtctt
     2281 tcagcgtcac cagggtctgc atctgcaact ccggcgccgc ctcctgctgg gcgctgatcg
     2341 taaaggtccg aaccatctgg acatgcccga tgtagccatc cttgccgcca taatatttgg
     2401 ttacgctgaa aggcgcaaga tcggcataca gggaccagtt ctcgaaggcg tccgccttga
     2461 caagactgtc tgcataaagt aaagctcgct gggtgatctt tgccgtgtcc ggctgtgcct
     2521 ggctgaccgt tgcggtcaaa aagaggatga tcccggataa gagtttcagg attttcatgg
     2581 gtattgattt gggcaccaat atactgcaaa attcctcccc atgcaacccc atgcccgata
     2641 ggatattatc aatatgtttt taaattcata aaaatctggg cctaaggcaa cacgagatca
     2701 ggaaaagccg tttttttcta gtaacttgac ccgcgtccaa caatcgccta tttattttcg
     2761 tctatgtcac agagatccga ttttgtctat tccgctaagc aggagcccca ccggcttcgt
     2821 accaaacaaa ttttacagca gcatccggaa gtgagaaagc ttatcggaaa gaacaagtac
     2881 acgattttcg cagtcctggg actcgttctc ttccaggttg tcctggcctg ggtggtaaaa
     2941 gaccgctcct ggtggatggt cgtcggcgca gcctacctgc tgggcgcctt tgccgatcac
     3001 tccctgttcg ttatgatcca cgagtgcgcg caccacctgc tgttcaaaaa ccgcgccgcc
     3061 aaccggctcg ccggcatcct cgccaacctc ccccaactat tccccagttc cgtttccttc
     3121 gaacggtatc atataaaaca ccactccttc cagggagtac acgaactcga cgccgatctt
     3181 cccaatcgct gggaagccaa actcataaat aactacttca tcggcaaagt gatctggctc
     3241 ctcttctacc ccctcttcca ggtgttccgc atctcgcgcc tccgcgaaat aaaacccttc
     3301 gacaaatggg tagccctcaa ctggaccgcc gaagtcctct tcgtcgccgc aatctggtac
     3361 ttcctcggcc ccaaagctat tgtctacctg ttgcttagct tcttcttctc cgtcggcctc
     3421 caccccctgg gcgcccgctg gatccaggag cactacctca ctcacgacga acaacaggag
     3481 accttcagct attatggagt gctcaacacg gtcgccttta acgtcggcta tcacaacgaa
     3541 caccacgact tcccctccat cccctggaat aaattgcccc agataaaaaa gacggccccc
     3601 gactactacg atagcctcgt gtcgcataaa tcctggaccg tcctcttctt ccgcttcctt
     3661 ttcgacaaag agatctcgct ctactcccgc atcgtccgca aggagagagg taaggttgcg
     3721 ctcaccgacg aatcaagacc cgatatggac ctcgtttccg ccacacagac agcaccaggc
     3781 cctgcccatc atgcacccgg cgcagcagtc atcacgcagg cctgagattc catgcgcagc
     3841 cgtcatcacc ctggcctgaa ctcccggctg gcgcctgaac agcctaaata aaaaccctgg
     3901 tcccggaatt gaaattctcc ctgaactcct ttggagtaca ggtcttcttc ttcttgaaga
     3961 tgcggttgaa gttggagata ttgttgaaac cgcagtggta ggagatctcc gaaatggaat
     4021 gcgtcgtatc gatcagcatc cgcgacgcat gacccagcct gatctcattc agactatcga
     4081 taaaagtatt ccccgtccgt tttttgatga atcggctaaa ggacacctcc gtcatactta
     4141 ccagcctcgc cacttcccca agcgtgatcg gcttgtcata attgttgttc atatactcaa
     4201 aggccttctc gatccgccgg ctgttatacg taaaatgctg gttggtaaag gaagcatccg
     4261 acagggtccg catattccgc gaaatggaca gatcatgcag gatcgataac aactccagca
     4321 cagaatcgaa accgttcttc tgattgaggg tcatgatccg cggcgccagc ctcgtagccg
     4381 tctcccgcga aaaaaggatc cccttctggc tccggtccat catattacgg ataaaactaa
     4441 gttgattgcg ccgcaacagc ttctcctcga acaggtcctt atgccattgg atcgttacct
     4501 cccggatctc ctcgctcttg cactgatgcg taaaccaggc atgataaaga ttcggaccca
     4561 cgagcaccag ttcgaggtcc tcgatggtgt cgatatggtc accaacgatc cgccttgcac
     4621 ctttcgcatt gattatcaga tttaactcaa gctcttcatg ataatgtaag gggaaactga
     4681 actctttttt aacccgcgag aagatggtga aacaatcatt ttgcgttagt ggtgtgatct
     4741 cacggataat gttcgtactc atatggcagt cgctcttttg aaagttaact aaagatacaa
     4801 tatccagaca atcgacgaat tctgataaaa aagggataaa atagtataac cctgaggtag
     4861 atacgaaaac aaccaacaaa acattactag ccaataacct ataccgccaa gcgcagactc
     4921 cccgtcaaaa atgaacaatt tctggataat aaagtagtac tcgggccgct gaaaataaga
     4981 taacttactt cccatgagac atctcttttt gtggatctgc tgctttccgt taaaattcag
     5041 atgaaactcc agctaccgga cttcgccatc ctcatagcct acctggtcac catggtcgtg
     5101 atcggctgga acctgcgcaa aaaagcccgg caaaataaac tgaactatct gctgggagga
     5161 aaatcccttc cctggtacat gctcgggctg agcgatgcct ccgatatgtt cgatatctcc
     5221 ggaacaatgt ggatggtcag cctctgtttc gtatacggga tgaaaagcat ctggatcccc
     5281 tggctctggc ccgtattcaa ccaggtcttc ctgatggtct atctctcccg ctggcttcgc
     5341 cggtcaaacg cgaccaccgg cgccgaatgg ctcgccaccc gctttggccg gtcaggcccc
     5401 ggcgtgcgcg cttcacacaa tatcgtcgtc gccttcgcct tgctgagctg cttcgggttc
     5461 atggcctacg gcttcgtagg gctgggtaag ttcatgcaga tctttatccc ctgggacagc
     5521 ataaaaggat ctctcccctt ccacatcccc cccgcctatg tcccccattt ctacggca
//
LOCUS       DATGFG010000439         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_518118, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000439 DATGFG010000000
VERSION     DATGFG010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518118"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1291
                     /locus_tag="VKQ52_08170"
     CDS             161..1291
                     /locus_tag="VKQ52_08170"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLZ87200.1"
                     /translation="MTPTDRLNSVLRYIKQAFIGKDEIVDLLGISLVAKENLFLLGPP
                     GTAKSAIVRLLSQCLEDGRNFEYLLTRFTEPNEIFGPFDIRRLKEGELVTNTEGMMPE
                     ASFVFFDEIFNANSAILNSLLMALNERIFRRGKETRRLPALMFVGASNMLPEDEALAA
                     LLDRFLLRVKCDYVDPDKLQEVLLAGWRLESESPAEKPVIHASTIIALQQECRQVDLA
                     PVRKQYIDIVHSLRSTGIKVSDRRAVKLQNIIAASALISQRRTAILSDLWVLKYIWDT
                     EEQIEILSGIIDSIIGKDAEGPAHPQTLYNRAPDPEEIMKEVAFLSSKWNGEALSFEE
                     RNVIKDKLRFVQNRCNWIKDAGQKTYIQKEIEGLWQHILQTS"
     gene            1264..2019
                     /locus_tag="VKQ52_08175"
     CDS             1264..2019
                     /locus_tag="VKQ52_08175"
                     /inference="COORDINATES: protein motif:HMM:NF039751.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87201.1"
                     /translation="MATHSPNLMIWWLEIDEAHLDHLRPVRHWGHLSMTAGPGVYWVK
                     DLRPDDPDRKEIRSIPFKRILYLRDDHLFPAGSLVPLRQVPGGLQWIPLEKGLPLQLP
                     SFNHNYFGVGERVTIRLVPSSKEEPPAALLVPTKLLQAYADTASSIRLRHLNWVVLDG
                     EALVIGAPILPLPGEVYWRQGDHLLPAGFGFAFPVLAEIAGLRLNLGGGLLLWDKAGT
                     CLPIAAGVWRPLSRSSVRLTGSDPDHHRSSLTS"
     gene            2021..>3348
                     /locus_tag="VKQ52_08180"
     CDS             2021..>3348
                     /locus_tag="VKQ52_08180"
                     /inference="COORDINATES: protein motif:HMM:NF039748.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87202.1"
                     /translation="MNFRQYFQPSVNYFWRPEAEDGTTVFCIPGDGTIVYSGFLMRVL
                     EDLSSQGLPPLGALLLALTATNHSQGDPLQKVEDILRSAVTGFRVDNPSVFGHMLDEA
                     MDFLRTLAALPLKYTGGEQRIQLFQVLFKGCHRQMSVEGSRDLLQAARQWIGETGKVR
                     EIPHELLTPARYTHDLKCLGLLSRQFPDEASIIGKLTTVPAIPGEILPEEEKEPGPAD
                     FVDSLIEQYSTFPIGILIRSIWSGLNIPIHHRVPGEQPLGGFSDLSNKGSFDKLLVSE
                     FANDEWLLLSRLANNEALFLHREIPPGADDLRRILLLDISLKSWGTPKVLAYAVLLAI
                     ARHPRTDIPCTAFAVGDDCYPIAFDTVDDLIGSLKRLDGCLHPAGGLAKFFGKRTGKD
                     KLEIIFIAPPDSHAHPAVKKVVSDHYSAFRYWVAVGREGEIDLYKNQNNSR"
BASE COUNT          697 a          912 c          980 g          759 t
ORIGIN      
        1 aagtgatcct ggccgatgac tgtgtaaaac ggttgtacgt cgaccgggtc atcggccgga
       61 aagcaatatc gcttgcgggg ctaccggtgc tgcgacggga agtggatgca gcgctgggtg
      121 actgggccac ttatttctgg aaagaattat cgttgaaaat atgactccta cagatcgttt
      181 aaatagtgta ttgcgctata tcaaacaggc ttttatcggc aaggacgaga tcgtcgacct
      241 gcttggcatc agccttgtcg caaaggaaaa tcttttcctt cttggtccgc cgggcacggc
      301 taagagcgcc atcgtccggc tcttgtcgca atgtctcgaa gatggccgca acttcgaata
      361 cctgctaacc cgtttcacgg agccgaacga gatctttggg ccctttgata tccgcagact
      421 gaaggagggg gagcttgtca ccaatacgga aggtatgatg cccgaagcct cgtttgtttt
      481 tttcgatgag atcttcaatg ccaatagcgc catcctcaac agcctgctga tggcgttgaa
      541 cgagcggatc ttccgtcggg ggaaggaaac ccgtcgcctt cctgccctga tgtttgtcgg
      601 ggcgagcaat atgctgcccg aggacgaggc gcttgccgcc ttgctcgacc gttttctgtt
      661 acgggtgaaa tgtgactatg tcgatccgga caaactgcag gaggtgctgc ttgcgggctg
      721 gcggctggaa tcggagtcgc cggcggagaa accggtcatc catgcgtcga ccatcatcgc
      781 gcttcagcag gagtgccgcc aggtggatct tgcgcctgtc cggaagcagt atatcgacat
      841 cgtgcatagt cttcgcagca cggggatcaa ggtctccgac cgccgcgcgg taaaattgca
      901 gaatatcatt gcggccagcg cgctgatcag ccaaaggagg acggccattc tctccgatct
      961 ctgggtattg aaatacatct gggacaccga agaacagata gagatccttt ccgggatcat
     1021 cgattccatt atcgggaagg atgccgaggg gcccgcgcat ccgcagacgc tgtataaccg
     1081 ggcgcccgat cccgaggaga tcatgaagga agtggccttt ctgtcctcga aatggaacgg
     1141 cgaagcgctt tcgttcgagg agcggaatgt catcaaggat aaattacgtt tcgtacagaa
     1201 tcgctgcaac tggataaagg atgcggggca gaagacctat atccaaaagg aaatagaggg
     1261 actatggcaa cacattctcc aaacctcatg atttggtggc tcgagatcga tgaggcgcac
     1321 ctggatcacc tgcgtcctgt ccgtcattgg ggtcacctat cgatgaccgc cgggcccggt
     1381 gtctattggg taaaggacct gcggcccgat gatcccgaca gaaaagagat caggagtatc
     1441 ccatttaaga ggatactcta cctgcgcgat gatcacctct ttcctgccgg cagcctggtg
     1501 ccgctgagac aggtgcccgg cggcctgcaa tggatacccc tggagaaagg actgcccctg
     1561 cagctgcctt cctttaatca taattatttt ggtgtcggtg aacgggtgac gatccgcctt
     1621 gtcccttctt caaaagaaga gccgcctgcc gcgctgttgg tgccgacgaa actgttgcag
     1681 gcgtatgccg atacggcctc ttccatccgg ttgcgtcacc tgaactgggt ggtcctcgat
     1741 ggagaggcgc tggtgatcgg cgcgccgata ctgcccctgc ccggcgaggt gtactggcgg
     1801 cagggagatc atctcctgcc ggccggcttc ggctttgcct tccccgtcct ggcggagata
     1861 gccggcctcc ggctcaacct tggcggcggc ctgctgcttt gggataaggc gggaacctgc
     1921 ctgccgatcg ctgccggtgt ttggcggcct ttgtcgcgta gttcggtgcg gttgactggt
     1981 tccgatcctg atcatcatcg ctcatcgtta acctcctgac atgaatttcc ggcaatactt
     2041 tcaaccaagt gtgaactatt tctggcggcc cgaagcggag gacggcacga cagtcttttg
     2101 tattcccggg gatggcacca tcgtgtatag cgggttcctg atgcgcgtcc ttgaagacct
     2161 ttcttcccag gggttgccac cgctgggcgc cctgctgctt gccctcactg ctaccaatca
     2221 ttcgcagggt gaccctttac aaaaggtgga agacatcctc cggagtgccg tcacggggtt
     2281 tcgcgtggat aacccttccg tattcgggca tatgctggat gaggcaatgg acttcctccg
     2341 cacgcttgct gccctgccgt tgaagtatac cggtggcgag cagcggatac aactgttcca
     2401 ggtgttgttc aaaggctgtc accggcaaat gtcggtcgaa ggctcccggg atctcctgca
     2461 ggcagcccgg caatggatcg gtgaaacagg gaaggtgagg gaaatccctc atgaactgct
     2521 aacaccggct cggtatacac atgatctgaa gtgtctcggc ttattatcga ggcaattccc
     2581 cgatgaggcc agtatcatcg gaaagttgac gactgtcccc gctatccccg gggagatact
     2641 cccggaagaa gagaaggagc ccgggccggc tgattttgtc gattccctga tcgagcaata
     2701 cagcactttc cccatcggta tcctgatccg gtcgatctgg tcgggtctca atatccctat
     2761 tcatcacagg gtcccgggcg aacaacctct cggtgggttt tccgatctta gcaataaagg
     2821 aagttttgat aagctcctgg tctccgaatt cgcgaatgac gaatggctgc tgctctcgcg
     2881 cctggccaac aatgaagccc tttttcttca ccgcgagata ccgcccgggg ccgacgacct
     2941 gcggcggata ctcctgctgg atatttccct gaaaagctgg gggacgccga aggtcctggc
     3001 ctatgccgtg ctgttggcga tcgccaggca cccgcgcacc gatataccct gcacggcctt
     3061 tgcggtggga gatgattgtt accctattgc cttcgatacg gtggacgacc tgatcgggag
     3121 cctgaaacgg ctggatggat gtcttcatcc cgccggggga ctggcgaaat tcttcgggaa
     3181 gcgaacgggg aaggacaaac tggagatcat ttttatcgcc ccgccggaca gccatgcgca
     3241 tccggcggta aagaaggtgg tgagcgatca ttattccgcc tttcgatact gggttgccgt
     3301 cggccgggaa ggcgagatcg atctgtacaa gaaccagaat aacagccg
//
LOCUS       DATGFG010000440         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_369267, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000440 DATGFG010000000
VERSION     DATGFG010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369267"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2288)
                     /locus_tag="VKQ52_08185"
     CDS             complement(<1..2288)
                     /locus_tag="VKQ52_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ87203.1"
                     /translation="MKHTVISFAVLLVALSSFAQQTRYFDDPQAEFRQGKEFFEHDFY
                     SLAYPIFRDLKYTLRETDKSNNSVEYQDVKYYYLVCALEQNDKIAVDAARDYIELENN
                     APRVGMISFHLAEYYFRQKDYASAVKLYDHADIDNLSNREIADLKFHEGYCYFTTRQF
                     DNALPLFNAIRQMPKDPNYLDANYYYGFICFYQKKYKDALEAFTVVENTPTYEKVVPY
                     YVANIYLVQGEKDKAIAYAEAHLKKGNQYYDAELRQLVGHGYYEKQDFVKALPFLEQY
                     VNQSKKVSREDLYELSYCYYQTKDWNKAISGFKQLGGASDSMSQNAMYLLGDAYLRTG
                     QKANARNAFLFCSMNSSNPKQKEISRFNYAKLSYELGYQDVALTELQGFLQQYPNSEY
                     AREGRELLVSVLAGTNNYKDALTLLDSLDHPSENARRLIPRILYGRATELVNDGMLLN
                     ANDLLDKALKDPNNGSVLPLVEFWKGEISYRLNKIDDAVRFYIEYLKTPVVNGEVNPT
                     NAYYNLGYCFLKKENYRQAQGFFEKVVRTPSLSSSPMEQDAYVRDADCYYMNRDYRTA
                     MTMYNKVLDLSWPASDYATFQKAMIAGVNNGKQKIELLNSISRRYPASSLSADVNMEV
                     AGTYLAGEQYREALPYLKNVLHTTGADALKPKAYLQSGIAWYNLNNNQEALKQYDSLL
                     RQYPNSPEAQDGLDNAKTIYVEEGRSSEYVNFAKGMGVEVSASQEDQLAYEEAEVQFN
                     NGNFPGAVQRFEAYLAKFPDGK"
     gene            2486..2908
                     /locus_tag="VKQ52_08190"
     CDS             2486..2908
                     /locus_tag="VKQ52_08190"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLZ87204.1"
                     /translation="MKKFFSTAYSESAFNLAALILRVTFGLILCFYYGYDKLRNFSHL
                     QYVFPDPIHLGHRISLALVIFAEFLCGILVALGLFTRFAALALVISMGVAEFLVHRGH
                     VAYMQGIIHEQAWLYFAAFFAILLVGPGRISVDGAMGR"
     gene            2937..3356
                     /locus_tag="VKQ52_08195"
     CDS             2937..3356
                     /locus_tag="VKQ52_08195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLZ87205.1"
                     /translation="MYTSETKVRVRYAETDQMNVVYHGNYAQYFEVGRVEAIRQLGLS
                     YKDLEATGIIMPVIEWTAKFVRPAHYDDLLTVRTTLREWPANHRIEFLQEVYNEKGKL
                     LTTGNVVLYFLRAETMEKTKMPEEMEARLKPWFTPGA"
     gene            3391..>3570
                     /locus_tag="VKQ52_08200"
     CDS             3391..>3570
                     /locus_tag="VKQ52_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLZ87206.1"
                     /translation="MNKVVAGIITIGDELLIGQVIDTNSAWMAQELNKSGIWVAHRIA
                     VGDIREDIWQALDQEF"
BASE COUNT          840 a         1046 c          834 g          850 t
ORIGIN      
        1 tattttccgt cggggaattt tgccaggtag gcctcgaaac gctgaacggc gcccgggaaa
       61 ttgccgttat tgaactgaac ctccgcttcc tcataagcca gctgatcttc ctggctggcg
      121 ctcacttcga cccccatccc cttggcaaaa ttgacatact cgctgctccg cccctcttcc
      181 acatagatgg tcttcgcatt atcgagccca tcctgcgcct cgggcgaatt gggatactga
      241 cgcaacaacg aatcgtattg cttgagagcc tcctggttat tgttcaggtt ataccaggcg
      301 atccccgatt gcagataggc cttcggcttc aacgcatcgg cgcccgttgt atgcagcaca
      361 ttcttcaggt agggcagcgc ctcccggtat tgctccccgg caagataagt accggcaacc
      421 tccatattta catccgccga caggctcgag gcgggatacc gccgggagat gctgttcagc
      481 agctcgatct tctgttttcc gttattgaca cccgcgatca tcgccttctg gaaagtggca
      541 tagtcactgg ccggccacga aagatccaac accttgttat acatcgtcat cgccgtccgg
      601 taatcccggt tcatataata acagtccgca tcccgcacat acgcatcctg ctccatcggc
      661 gaagagctca gactcggcgt cctgaccacc ttctcaaaaa agccctgcgc ctggcgataa
      721 ttttccttct tcagaaaaca atatcccaaa ttataatagg cattggtcgg gttcacctcg
      781 ccgttgacaa ccggcgtctt caggtattcg atataaaagc ggacagcgtc gtcgatcttg
      841 ttcaaccggt acgatatctc tcccttccag aactcgacca gcggcagcac gctcccattg
      901 ttcggatctt tcagcgcctt gtccagcaga tcgttcgcat tcagcaacat cccgtcatta
      961 accagttccg tcgcccggcc atacaggatc cgcgggatca gccgccgggc attctccgaa
     1021 ggatgatcaa gactgtccag cagcgtcagc gcatccttat aattattggt ccccgccagc
     1081 acactcacca gcaactcccg cccttcccgg gcatactcgg aattgggata ttgctgcaaa
     1141 aagccctgta gctccgtcaa cgcgacatcc tggtacccta attcatagga taacttggcg
     1201 tagttgaacc gggatatctc cttttgtttg ggattgctgc tgttcatgct gcagaacagg
     1261 aaggcattcc gggcgttggc cttctggccg gtacgcagat aggcatcccc aagaagatac
     1321 atcgcattct gcgacatcga atccgacgcc ccgcccaatt gcttgaaccc gctgatggcc
     1381 ttgttccagt cctttgtttg ataataacag taggacagtt cataaaggtc ctcgcggctc
     1441 actttcttcg actggttgac atactgctcg agaaagggca gcgccttgac aaaatcctgc
     1501 ttctcataat acccatgccc caccagctgc cgcagttcgg cgtcataata ttgattcccc
     1561 ttcttcagat gggcttccgc ataagcgatg gccttgtcct tctccccctg aacaagatag
     1621 atgttagcca cataataggg aacaaccttc tcataggtgg gcgtattttc tacaaccgta
     1681 aaagcttcca gcgcatcctt gtatttcttc tggtagaaac agataaaccc gtaatagtaa
     1741 ttggcatcga gatagttggg gtccttcggc atctgccgga tggcattgaa tagcggcagg
     1801 gcattgtcaa actgccgggt ggtaaaataa cagtacccct catgaaattt cagatcggcg
     1861 atctcccggt tgctcagatt gtcgatatcc gcatggtcat acagcttcac cgcgctggca
     1921 taatccttct gccggaaata gtattcggcc aggtgaaaac tgatcatgcc caccctgggc
     1981 gcattgttct ccagctcgat atagtcccgg gcggcgtcca cggctatctt gtcgttttgc
     2041 tccagggcac acaccaggta atagtatttt acatcctggt actcaacgga attattgctt
     2101 ttgtcggtct cacgaagcgt gtacttcagg tcgcggaaga taggataggc aagactatag
     2161 aagtcatgct cgaagaactc cttaccctgg cggaattcgg cctgcgggtc gtcgaaataa
     2221 cgcgtctgct gggcgaatga tgaaagagca acgagaagga cggcaaacga tattacagta
     2281 tgcttcactg gaaaaaattt gatttttatc atcagtcttc accggggccg caccaacagg
     2341 cctaacgcgc taagtttcta cttattttgc cttttaccaa acatcgttcc aaacacctgt
     2401 ggataactgc aaggattaac atttccttat aagtctttgc accacagctc gcaattccta
     2461 tcttcacggg caaattcaat cgtacatgaa aaaattcttc tccaccgcct attccgagtc
     2521 cgccttcaac ctggcggccc tgatcctgcg cgtgaccttc ggcctcattc tctgcttcta
     2581 ctatggttat gacaaactgc ggaacttctc ccatctccaa tatgtcttcc ccgatcccat
     2641 ccatctcggc caccgcatct cccttgccct ggtcatcttc gccgaattcc tctgcggaat
     2701 cctggtggcg ctgggcctct tcacccggtt cgccgccctc gcactggtca tcagcatggg
     2761 tgtcgctgaa ttcctggtcc acagaggaca cgtcgcctat atgcagggaa taatacatga
     2821 acaggcctgg ctgtatttcg ccgccttttt tgccatcctg ctcgtaggcc ccggccgcat
     2881 cagcgtagac ggcgcaatgg gcagataagc accggaccga tcaaccgatt cagactatgt
     2941 acaccagtga aacaaaggtc cgcgtacgct atgccgaaac cgaccagatg aatgtcgtct
     3001 atcacggcaa ctatgcccaa tacttcgagg tcggccgggt agaagcgatc cgccagctgg
     3061 gcctgagcta taaggacctg gaagcaaccg ggatcataat gcccgtcatc gaatggaccg
     3121 ccaaatttgt ccgcccggcc cattatgatg atctgctcac cgtccggacg accctacggg
     3181 aatggccggc aaaccaccgg atcgaattcc tgcaggaagt ctacaacgaa aaaggaaagc
     3241 tgctcacgac aggcaacgtg gtcctgtatt tcctccgcgc cgaaacgatg gaaaaaacaa
     3301 agatgccgga ggaaatggaa gcaagactaa agccctggtt cacccccggc gcataaccgg
     3361 gcggaagccg atttccttta cctttaccaa atgaataagg ttgttgccgg catcatcacc
     3421 atcggagatg aactgctcat cggacaagta atagacacca acagcgcctg gatggcgcag
     3481 gaattgaaca aaagcggtat ctgggtcgcc caccgcatag ccgtcggcga catccgcgaa
     3541 gacatctggc aggcattgga ccaggagttc
//
LOCUS       DATGFG010000441         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1546888, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000441 DATGFG010000000
VERSION     DATGFG010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546888"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VKQ52_08205"
     CDS             <1..1304
                     /locus_tag="VKQ52_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HLZ87207.1"
                     /translation="FKKKMTVTFEQYYERTDRWLEMSLYPSEQGVFAYFKEVTQRKKQ
                     EALLALEKKVLELNTAKQMSLRGLLNFFLKGIQKIFPGMYCCVLTLDEDNLSVRLLSA
                     PGLPAVYAHAIDGLPIGPKAGSCGTAMYRKETVIVPDIANDPLWEEQRDLTLQFGLRA
                     CWSIPVINGRGEVMAAFAVYYTTVKSPTDTEMDTFERVANLVAMIIESKKAEELLLKQ
                     ELTKQKMIAQAMVDAQEKERAEIGKELHDNINQILSTTKLYLELAKNDPKERLNLINR
                     SAGNIHNAIREIRNISRSLVPASIGDLGLQDSIADLVESLRTTKAINVEFYSAGSIDE
                     KISDKAKLMLFRIIQEQVNNVLKHSGARNLIIELILEDADQRIELSITDDGKGFDPGK
                     AGKKGLGLSNITSRADLFGGKVTIQSAPGRGCKLRVQVPVI"
     gene            1325..1966
                     /locus_tag="VKQ52_08210"
     CDS             1325..1966
                     /locus_tag="VKQ52_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87208.1"
                     /translation="MSKINILIADDHKLIRETWSYILNSDARFQVIAECGDAQEAVEL
                     AKTKRPHVVLMDINMTPFSGLEATQRIRKISPGSKVIGVSMHSQPAYAKKMLQMGARG
                     YVTKNSSKEEMIKAILEVNHGNKYICDEIKNIISEQLLDEKEDSPNINALTEREMQII
                     NFIKEGLSSKEIATSLNISLKTVEVHRHNILKKLKLKNSASLVNFINTNATYI"
BASE COUNT          673 a          635 c          524 g          480 t
ORIGIN      
        1 ccttcaaaaa gaaaatgacg gtcacttttg aacaatacta cgaacgtaca gaccggtggc
       61 tcgaaatgag cctctatcct tccgagcagg gagtgttcgc ctatttcaag gaagtgaccc
      121 agcgcaaaaa acaggaggcg ctgctggccc tcgaaaagaa agtgcttgaa ctgaacacgg
      181 ccaaacaaat gtccctccgg ggccttctta atttcttcct gaagggtatt caaaagatct
      241 tccccggcat gtactgctgc gtcctcacac tcgatgagga caacctcagc gtgcgactgc
      301 tgtccgcacc cggactgccg gcggtttacg cccacgctat cgacggcctc ccgatcggcc
      361 ccaaagccgg cagctgcggc accgccatgt accgcaagga aaccgtcatc gtcccggata
      421 tcgccaacga tccgctatgg gaagaacaac gcgacctgac cctccaattc ggtctccgcg
      481 cctgctggtc cattcccgtt ataaacggcc gcggggaagt aatggccgcg tttgccgtct
      541 actacaccac cgtgaaatct cccaccgaca cggaaatgga caccttcgaa cgggtggcca
      601 acctcgtagc gatgatcatc gagagcaaaa aggcggaaga actcctgctc aaacaggaac
      661 tgacaaaaca aaaaatgatc gcccaggcca tggtcgacgc ccaggaaaaa gaaagagcgg
      721 agatcggaaa agaactgcac gataatatca accagatcct ctccaccacg aaactctatc
      781 tcgaactggc aaagaacgat cctaaagaac ggctgaacct tatcaaccgc agcgccggaa
      841 atatccataa tgccatccgg gagatcagga acatttcacg tagcctcgtt ccggcaagca
      901 tcggcgacct cggactccag gactccatcg ccgacctcgt ggaaagcctg cgaaccacca
      961 aagcgatcaa cgtcgaattc tattccgcag gcagtattga cgaaaagata agcgataaag
     1021 caaaactgat gctcttccgc atcatccagg aacaggtgaa taacgtgctc aaacattccg
     1081 gggcgcgtaa tctcatcatc gaactgatcc tggaagacgc cgaccagcgg atagaactca
     1141 gcatcaccga cgacggaaaa ggtttcgatc ccgggaaagc cggaaaaaaa ggactcggcc
     1201 tgtccaatat caccagcagg gcagacctgt tcgggggcaa ggtcaccatc caatccgcgc
     1261 ccggccgcgg ttgcaaatta agagtgcaag taccagtcat ataacaatct taaaacaatc
     1321 ctatatgagt aagatcaata tcttaattgc tgatgaccac aaactgataa gggagacctg
     1381 gagttatatc ctaaacagtg acgcccggtt tcaggtgata gcggagtgcg gcgacgctca
     1441 ggaagcggtc gaactggcaa agaccaaaag gccacatgtc gttctcatgg atatcaatat
     1501 gacccccttt tccggtctcg aagcaaccca gcgcatccgg aagatctcgc ccggctcaaa
     1561 agtgatcggc gtatccatgc actcccaacc cgcctatgca aagaaaatgc tccagatggg
     1621 cgcccgcggc tacgtgacaa aaaactcttc caaagaagaa atgataaagg cgatcctcga
     1681 ggtcaatcac ggcaacaagt atatctgcga tgagataaag aacatcatct cggaacaact
     1741 gctggacgaa aaggaggatt ctcccaatat caatgccctg accgaaagag aaatgcagat
     1801 catcaacttc atcaaggaag ggctctcctc gaaagagatc gccaccagcc tcaatatctc
     1861 cctgaagacc gtggaggtcc atcgtcacaa tatccttaag aaactaaaac tgaagaattc
     1921 ggcctccctg gtgaatttca tcaatacaaa cgctacatat atttagaaaa cacctataac
     1981 tcattgattt ttcccttatt gtggataaat ctgcgcgcat ttgcttcgac tttccgggac
     2041 tttgatgcat ttttgtcggg aaaccaattc ctacgaaacg ccagggctca aggttcctgg
     2101 aaaagagtgt tcaaattcct gttgtacacc gcccccaatc ctatgaagac tgggggtttt
     2161 tttgcctccg cccatctccg cttcttttat ttttctttaa ggaataagtt ggtaactttg
     2221 cggttcattt ttcatagatg agtgatgaaa taaaacatga atgcggtctg gcattcattc
     2281 ggttacgcaa gccgttctcc tattatctac ag
//
LOCUS       DATGFG010000442         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_694539, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000442 DATGFG010000000
VERSION     DATGFG010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694539"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VKQ52_08215"
     CDS             <1..1090
                     /locus_tag="VKQ52_08215"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87209.1"
                     /translation="AYYNTGDATSWFQKAHDMAMQVINNQSQYNISLYTNYADMWNPA
                     NRQGSSNSKVNKEAIFWATYSTNTAIDINGNGNRTNLWFLTNYSGYSGTTIPGLTISI
                     QYGNDQKNRRFMPTRALLNFYNDTIDSRYAGSFQEIWLCNKAYTWTATDVKTFGKDPS
                     LVGHKMNVGDTAMWISKHVIPNRSKRAYACFGVDTTYNADGTISNGGGDHYVVLTKYL
                     DPLTKAGTTTYPGYLDLPILRLAEQYLIAAEADVQLNNPGEAATLVNVLRTRAAVKTP
                     VNHTADMQVNASQMTLDFILDERARELCGEYMRWFDLKRTGKLQSRIANYNPDITNFN
                     PNYYLRPIPLVEIQGLTNGAAFGQNPGY"
     gene            1090..3960
                     /locus_tag="VKQ52_08220"
     CDS             1090..3960
                     /locus_tag="VKQ52_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87210.1"
                     /translation="MRLTFILLLSSLSCLGQAPAASGLHSSAPPGLSRLQYGKQRLAA
                     VAGGGRSLVIKIDSSIGQKEGYTIDVNGNTARITGADASGALYGCLTLADKIKTGGWP
                     RSLHLADHPRMILRGACIGLQKPTLLPGRGPYEYPYTPQNFPWFYDKALWLKYLDSMV
                     DNRLNTLYLWNGHPFASLVRLPDYPYAVEVDDSTFKKNEAIYHWLTTEADRRGIWVIQ
                     LFYNIIVSKPFAEHNHLKTQDRNRHIIPIIADYTRKSIAAFVEKYPNVGLMFTLGEAM
                     EGVGQDDIDWLTKTIIPGIKDGLRAMGRTDEPPVVLRAHDTDGPADLRAALPLYSNLY
                     VEAKYNGEALTTYTPHGPWADLHRELSSLSKGLIENVHIMANLEPFRYGSPDFIQKCV
                     QAMHSVYGANGLHLYPQASYWDWPYAADSVSPGSPAPSSGTPASADSLTPSGSHETLP
                     AGRLLEMQRDWIWYKAWARYAWNSDRPRDEEIKYWVTQLQAKYGCDSTHARAVLDAYE
                     QSGEISPKLLRRFGITDGNRQTLTLGEFMTQLIDPERYGLFTLLYESEGPPGETLAEY
                     AEKEWKHLPHTGETPVDVINDVREEGRLAVSAIDRAAAPNTPASPNLPAATRPRNAAN
                     RATSAITANRAEFTRLRNDMYCYRALAGFYAAKAEAALHVLRFKYSNDLTDLQNALPP
                     LQTSVDQFRELTRLAAPAYRYANSMQTAQRKIPIRGANGRFITWQELLPLYEEELQHF
                     KTHLDSLKTSTGQPIAAPQPFQPATVQLAAGPGTTPTATNNTPTTAANPTSPTITTSG
                     APLLTYTPDIGAHPFTDTVLAIRAATPVLKNLGAIAQSSITQRKQGTKITFTCSEPVD
                     LLIGFFNQKGPAFLPPPQLETDASANDYGQAEPRITNALIIAGMPPVNIHAWSFQPGT
                     HTLELGKGLCLVLGFAKPLTKTKPFDAGLNEPGKKNLDWLFE"
BASE COUNT          929 a         1499 c          879 g          660 t
ORIGIN      
        1 cgcctattat aatacgggtg acgcgacttc ctggttccag aaagcccatg acatggccat
       61 gcaggtgatc aacaatcagt cgcaatacaa catcagcctc tataccaatt atgcggatat
      121 gtggaatccc gccaaccgcc agggcagcag caactcgaag gtcaacaagg aagcgatctt
      181 ctgggctacc tattccacca acacggccat cgacatcaac ggcaacggca accggaccaa
      241 cctctggttc ctcacgaact acagcggcta cagcggtacg accattcccg gcctgaccat
      301 cagcatccaa tacggcaacg accagaagaa ccgccgcttc atgcccaccc gcgccctgct
      361 caacttttac aacgacacca tcgactcccg ctatgcaggc tccttccagg agatctggct
      421 ctgcaacaaa gcctacacct ggacggcgac agatgtaaaa accttcggga aggaccccag
      481 cctggtcggt cacaaaatga acgtcggcga caccgccatg tggatcagca agcacgtcat
      541 ccccaaccgg tccaaacggg cctatgcctg cttcggcgtg gatacgacat acaatgccga
      601 tggcacgatc agcaatggcg gcggcgacca ctatgtcgtg ctgacaaaat acctcgatcc
      661 gctcacgaag gccggcacca cgacgtatcc aggttacctc gatctgccca tcctccggct
      721 ggccgaacaa tacctcatcg ccgccgaagc cgacgtccaa ctcaataacc ccggtgaagc
      781 agccaccctc gtcaacgtcc tccgcacccg cgccgcggtc aagactcccg tgaaccatac
      841 ggccgatatg caggtgaacg catcacagat gaccctcgac ttcatcctcg atgaacgcgc
      901 gcgggaactc tgtggggaat acatgcgttg gttcgacctc aagcgcacgg gcaaactgca
      961 aagccgtatc gccaactaca atccggatat cacgaacttt aatccgaact attacctgcg
     1021 tcccataccc ctggtcgaga tccagggcct gaccaacggc gctgcatttg gacaaaaccc
     1081 cggttactaa tgagattaac gttcatctta cttctgtctt cactttcctg tctcgggcaa
     1141 gccccggctg cttccggcct tcactcctcc gcacctccgg gtctctcccg actccaatat
     1201 gggaaacaac gcttagctgc ggtcgccgga ggtggccgca gcctcgtcat taaaatcgat
     1261 tcctccatcg gccaaaaaga aggatacacc atcgatgtta acggcaacac cgcccgcatc
     1321 accggcgccg acgcctcggg cgccctctac ggctgtctga cgctcgcgga caaaataaaa
     1381 acgggcggct ggccaaggag cctccacctc gcggaccatc cccgcatgat tctccggggc
     1441 gcctgtatcg gcctgcaaaa accgaccttg ctccccggcc gcggtcctta cgaatatccc
     1501 tatacgccgc agaacttccc ctggttttat gacaaggcgc tctggttgaa atacctcgac
     1561 tccatggtcg acaatcgcct caacaccctc tatctctgga atggccatcc cttcgcctcc
     1621 ctcgtccggc tgcccgacta cccctatgcc gtggaggtgg acgacagcac gttcaaaaag
     1681 aatgaggcca tctaccactg gctcaccacc gaggccgacc ggcgcggcat ctgggtcatc
     1741 cagctgtttt ataatatcat cgtctcgaaa cccttcgccg aacataacca tctaaagacg
     1801 caggatcgca accggcatat catccccatc atcgccgact atacccggaa atccatcgcc
     1861 gccttcgtcg aaaaatatcc caacgtcggc ctgatgttca cccttggcga ggccatggaa
     1921 ggggtagggc aggacgatat cgactggctg acaaagacca tcatccccgg gatcaaagac
     1981 gggctccgcg ccatgggccg caccgacgag ccccccgtcg tccttcgcgc ccacgacacc
     2041 gacggccccg cggacctccg ggccgccctg ccgctctatt ccaatttgta cgtcgaggcc
     2101 aaatacaacg gcgaggccct taccacctat accccccacg gcccctgggc cgatctgcat
     2161 cgcgaattga gcagtctcag taaaggcctt atcgagaacg tccatatcat ggccaacctc
     2221 gaacccttcc gctatggctc ccccgatttt atccagaaat gcgtccaggc catgcacagc
     2281 gtctatggcg caaacggcct gcacctctat ccccaggcct cttactggga ctggccctac
     2341 gccgccgaca gcgtgagccc gggatccccc gcaccctcct ccggcacccc cgcctccgcc
     2401 gactccctca caccctccgg ctcccacgaa acgctgcccg cgggtcgact cctcgaaatg
     2461 caacgcgact ggatttggta caaagcctgg gcccgctatg cctggaacag cgaccgcccc
     2521 cgcgacgaag aaataaaata ctgggtaacg caactacaag caaaatacgg ctgcgacagc
     2581 acccatgccc gcgccgtcct cgacgcctac gaacagtccg gcgagatctc gccaaaactc
     2641 ctgcgccgct tcggcatcac cgatggtaac cgccaaaccc tcaccctcgg cgaattcatg
     2701 acccaactga tcgaccccga gcggtacggg ctcttcaccc tcctgtacga atccgaaggc
     2761 cctcccggtg agaccctcgc cgagtacgcg gaaaaagagt ggaagcacct gccccacacc
     2821 ggcgaaaccc ccgtcgatgt catcaacgac gtccgcgagg aaggccgcct tgccgtcagc
     2881 gccatcgatc gcgccgcggc ccccaacacg cccgcatccc ccaacctccc cgcggccact
     2941 cgcccccgca acgccgccaa ccgcgcaacc tccgccatca ccgccaaccg cgccgaattc
     3001 acccgcctgc gcaacgacat gtactgctac cgcgccctcg ccggcttcta cgccgcaaaa
     3061 gcggaggccg cactccatgt cctgcgcttt aaatactcca acgacctaac cgacctgcaa
     3121 aatgccctcc cccctctcca gaccagcgtc gatcaattcc gcgaactgac ccgcctcgcc
     3181 gcccccgcct accgctatgc caacagcatg cagacggcac agcgcaagat ccccatccgc
     3241 ggcgccaacg gccggttcat tacctggcag gaactgcttc ccctatacga agaagaactc
     3301 caacacttca aaactcatct ggattctcta aaaacgtcca ccggccaacc catcgccgca
     3361 ccgcaaccct tccaacccgc caccgtccaa ctggcagccg gccctggcac aacacccacc
     3421 gccaccaaca acacccccac cactgccgca aaccccacct cccccacaat cacaacctcc
     3481 ggcgcacccc ttctcaccta cacacccgac atcggcgccc atcccttcac cgacacggtc
     3541 ctggctatcc gcgccgccac ccccgtcctg aaaaacctgg gcgccatcgc gcaatcttcc
     3601 atcacgcagc gcaaacaagg caccaaaatc accttcacat gctccgaacc cgtagatttg
     3661 ctcatcggct tcttcaacca aaagggcccg gccttcctgc cgccaccgca attggagacc
     3721 gacgccagcg ccaacgatta cggccaggcc gaaccgcgga tcaccaacgc actcatcatc
     3781 gcgggcatgc caccggtcaa tatccacgcc tggtccttcc aacccggtac ccacacgctg
     3841 gaactcggta aaggcctctg cctcgtactc ggcttcgcaa aaccgctgac aaaaacaaaa
     3901 cccttcgacg ccggcctcaa cgaacccggt aaaaaaaatc tcgactggtt attcgaataa
     3961 cgaatga
//
LOCUS       DATGFG010000443         3536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1698672, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000443 DATGFG010000000
VERSION     DATGFG010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698672"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1121
                     /locus_tag="VKQ52_08225"
     CDS             54..1121
                     /locus_tag="VKQ52_08225"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLZ87211.1"
                     /translation="MNIALITGSAGLIGSEAVSFFSPRFDLIIGIDNNLRQYFFGEGS
                     STEWNKNRLQEKFSNYRHYSPDIRDTSALEKIFTEYGADIKLILHTAAQPSHDWAARE
                     PFTDFSVNANGTLNLLEMNRLHCPEAVFIFTSTNKVYGDTPNYLPLREGETRWEIDEA
                     HPYYKHGIDELMSIDHTKHSLFGASKVAADILVQEYGRYFGMKTGIFRGGCLTGPNHS
                     GAKLHGFLSYLMKCAITGDQYTVFGYKGKQVRDNIHSFDLVNMFWEFYKNPTSGEVYN
                     AGGGRHSNCSMLEAISLCEEITGKKMNYIYTEDNRIGDHIWWISDVSKFKTHYPEWNW
                     KYGLMDILVQIKEATVSRVAH"
     gene            1136..1933
                     /locus_tag="VKQ52_08230"
     CDS             1136..1933
                     /locus_tag="VKQ52_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ87212.1"
                     /translation="MRLSVVIPAYNEEGSIEATVRTIHEALARNGISHQVLVVNDNSK
                     DRTQEILQALSDEIPEVNYINNPGANGFGFAVRMGLENFSGDCVAVMMGDLSDSPEDL
                     VRFYNKMLEGYDCVFGSRFIRGGKIYDYPFIKLVINRMANTIVQIMLGIRYNDATNAF
                     KLYRKETIQGLKPFLSHHFNLTVELPLKAIVRGYSYAVVPNSWTNRKTGESKLKIREM
                     GSRYFFILLYCLIEKFFSRGDYVKKPAPHASSEPVKNVSIPSPSPDR"
     gene            complement(1905..>3536)
                     /locus_tag="VKQ52_08235"
     CDS             complement(1905..>3536)
                     /locus_tag="VKQ52_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87213.1"
                     /translation="DLLLRKRASPGRLKETGETSTTEASLGEEVRIKRSNGIQFTQIF
                     VSYNRKGYLARALYQPPVLRITFTLENGQTLTQRAIKPILSDGVILNKYIDSRDEFRL
                     LMQTGGHLNTNVRSVRIDADPADWGFVKKIQLVTRYYLLTGATGVNEDSLALAGLTRE
                     YEQHPPRLIDPGKYIADSIRTGMDVSTTSSFFLRAEGWAFREKGDNHDILVQPVVRSG
                     DKVYELSAEKRQRPDLFFAFNRRDLDNAGFESSIMQTQLPPGQYQAGVAIYDHHDGKQ
                     YLGYTDHFFTVLKRFAVEGGGRTNPRFINKGNLQMAIDSIVDREDQVLIKGWAFIKGV
                     GSTSSAGWLILRGGREDYRIPVQTLPSEKVTGYFNDPRYGNCGFMVVVSKAALPEGVY
                     TLGIGMAAGSKDTTVLFDDRRLYIDTAESPAKIAGQPPVKDFPSGIDEVKDEKDFLTL
                     SGWAVAEQDTIDDDAIDIVLLSGDAIFIAPTQMRSRPDVKAALKSRLNADNSGFFAKI
                     PKAGLPAGKYRLGLYLHHGGGQGVMKFIDQGMVKE"
BASE COUNT          812 a          979 c          961 g          784 t
ORIGIN      
        1 caggtggacg accaggtaaa aaccattgaa acagacagca aactagtaaa acaatgaata
       61 tcgcattaat aaccggttcg gccgggctta tcggcagtga ggcggtgagc ttcttctctc
      121 cccgcttcga ccttatcatc gggatcgaca ataatctccg gcaatacttt tttggcgaag
      181 gcagttccac cgagtggaat aagaaccggc tgcaggagaa gttcagcaac taccggcatt
      241 acagccccga catccgcgat accagcgccc tggaaaaaat ctttacagaa tatggcgccg
      301 atataaagct gatcctgcat accgccgccc agcccagcca tgactgggct gcccgcgaac
      361 ccttcacgga tttttccgta aatgccaacg gaacgttgaa cctgctggaa atgaaccggc
      421 tgcactgccc cgaggcggtc tttatcttta cctccaccaa caaggtctat ggcgacacgc
      481 ccaattacct gccgctgcgg gaaggggaga cccgctggga gatcgatgag gcgcatccgt
      541 attacaagca cggcatcgac gagctcatga gcatcgacca taccaagcat tcccttttcg
      601 gggcttccaa ggtggccgcc gatatcctgg tccaggagta tggccggtat ttcggtatga
      661 agacgggtat tttccggggc ggttgcctga cagggcccaa tcattcgggc gcaaagctgc
      721 acggattcct gtcctacctg atgaaatgcg ccatcacagg cgatcagtat accgtcttcg
      781 gatacaaggg gaaacaggtg cgggacaata tccacagctt tgacctggtg aatatgttct
      841 gggagttcta taagaacccg acatcggggg aagtctacaa tgccggcggc ggcaggcatt
      901 ccaactgttc catgctggaa gctatttctc tctgcgagga gatcacaggg aaaaaaatga
      961 actacattta caccgaagac aaccgcatcg gcgaccatat ctggtggata agcgacgtga
     1021 gcaaattcaa gacgcactac cccgaatgga actggaaata tgggcttatg gatatccttg
     1081 tccagataaa ggaagcgacg gtgtctaggg ttgcgcacta aaacgacctt ataatatgag
     1141 attaagcgta gtcattcccg cctacaacga agagggctcc atcgaagcca cggtcaggac
     1201 catccatgag gcgctggccc gcaacggcat cagtcaccag gtactcgtcg tcaacgacaa
     1261 ttccaaagat cgcacccagg agatcctgca ggccttgtcc gatgagatcc ccgaggtgaa
     1321 ctatatcaac aatcccggcg ccaatggatt tggctttgcg gtaaggatgg ggctggagaa
     1381 tttttccggc gattgcgtgg ccgtgatgat gggcgacctg tcggactcgc cggaagacct
     1441 tgtccgtttc tataataaga tgctggaagg atatgattgc gtattcggca gccggttcat
     1501 acgaggcggc aagatctatg actatccctt cataaaactg gtcatcaacc ggatggccaa
     1561 taccatcgta cagatcatgc tgggtatccg gtacaacgac gctaccaatg cgtttaagct
     1621 gtaccgcaag gaaacgatcc agggcctgaa accctttctt tcccatcatt tcaacctcac
     1681 ggtcgagctt cccctaaagg cgatcgtaag aggttactcc tatgcggtcg ttccgaattc
     1741 ctggaccaac cggaagacgg gggagtcgaa gctgaagatc cgggagatgg gaagccggta
     1801 tttcttcatc cttctgtatt gtctcatcga aaagttcttc tcgcggggcg actatgtcaa
     1861 aaagccggcg ccccatgcct cttccgaacc cgtcaagaac gtctctattc cttcaccatc
     1921 ccctgatcga taaatttcat tacgccctga ccgccgccgt ggtggagata gagcccgaga
     1981 cggtatttgc ccgcgggcaa gcccgctttg gggatcttcg caaagaaccc ggaattgtcg
     2041 gcattcaacc tgctcttcag cgccgctttt acatcgggcc ggcttctcat ctgcgtgggt
     2101 gcgataaaga tggcatcccc cgaaaggagc acgatatcga tggcatcatc gtcgatggta
     2161 tcctgttcgg caaccgccca tccggaaagg gtaaggaaat ctttctcatc ctttacttca
     2221 tcgatacccg aagggaaatc cttcaccgga ggctggccgg cgatcttcgc cggtgattcg
     2281 gcggtgtcga tatagagtct cctgtcatcg aagaggacgg ttgtatcttt cgaacccgcc
     2341 gccattccga tgccaagggt atatacgcct tccggcaggg ctgcctttga caccacgacc
     2401 ataaagccgc agttcccgta gcgcggatcg ttgaagtaac cggtaacttt ttcgctcggc
     2461 agggtctgga cgggaatgcg gtaatcttcc cggccgccac ggaggatgag ccagccggca
     2521 gaggaggtgc ttcccacccc ctttataaag gcccagccct tgatgagcac ctggtcttcc
     2581 ctatcgacga tactatcgat cgccatttgc aggttcccct tatttataaa tcggggattg
     2641 gtcctgcccc cgccttcgac ggcgaagcgt ttcagcacgg tgaagaaatg atcggtgtat
     2701 cccaggtatt gtttaccgtc atgatggtca tagatggcga cccccgcctg gtactgcccg
     2761 ggaggcagct gggtctgcat gatggaggac tcaaaaccgg cattgtcgag atcccgccgg
     2821 ttgaaggcga agaaaagatc cggccgttgc cgtttttccg cggacagctc atatacttta
     2881 tcccccgaac ggaccaccgg ctgaacgagg atatcgtggt tatccccctt ttccctgaaa
     2941 gcccagcctt ccgcccggag gaaaaaagaa gaagtggtgg agacatccat cccggtacgg
     3001 atggaatcgg cgatatactt gcccggatcg atcagccggg gcggatgctg ttcatattcc
     3061 cgggtcagcc cggcgagcgc caggctgtct tcattgacac cggtggcgcc ggtgagcagg
     3121 tagtagcgcg tcaccagttg tattttcttt acaaaccccc agtcggcagg gtcggcatcg
     3181 atccggaccg accgcacatt ggtgttgaga tgaccgccgg tttgcatcag caggcggaat
     3241 tcgtccctgc tgtcgatgta tttattgagg atgaccccgt ccgagaggat cggttttatc
     3301 gcccgctggg ttagggtctg gccgttctcg agcgtaaacg tgatgcgcag caccgggggc
     3361 tgatagaggg cgcgcgcgag atagcccttg cggttatagc tgacgaagat ctgggtgaac
     3421 tggatgccgt tgcttctttt tatccgcacc tcctcgccca gggatgcttc cgtggtggac
     3481 gtctctcccg tctccttcag ccggcccgga gaagcccgct ttctaaggag caggtc
//
LOCUS       DATGFG010000444         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1678425, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000444 DATGFG010000000
VERSION     DATGFG010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678425"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VKQ52_08240"
     CDS             complement(<1..282)
                     /locus_tag="VKQ52_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87214.1"
                     /translation="MDAILIKGARVHNLKNIDVAIPRNKLVVVTGVSGSGKSSLTIDT
                     LFAEGQRRYAESLSAYARQFMARMNKPDVDYIKGLCPAIAIEQKVITRTP"
     gene            731..1312
                     /locus_tag="VKQ52_08245"
     CDS             731..1312
                     /locus_tag="VKQ52_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ87215.1"
                     /translation="MTLLRKKTDHELIHQFRDGDLYALEALIVRHKDKLYTSILFLVK
                     DKYLAEDIFQDVLIKIIDTIRGGRYTEEGKFLPWAMRIAHNLCVDHFRKVKRTPAIKT
                     SDDRDIFEVIHFTEEGADTKMMKRQSHDRVRQMLDLLPEDQREVIILRHYADLSFKEI
                     AALTNCSINTALGRMRYGLINLRKMMTEKKIAL"
     gene            complement(1679..>3471)
                     /locus_tag="VKQ52_08250"
     CDS             complement(1679..>3471)
                     /locus_tag="VKQ52_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLZ87216.1"
                     /translation="NSRVALDSSAKEKITGIEEKSPVQRKKEPLFNNAETRFFTGDAQ
                     WLVHLQPTGAAAGDLKGTVTFMAIYKDSVLGPVEIPFRYGVTAGGGLVAKAAGLQESA
                     SASSGLKKSTIDLANPVNSCGGTGGDTKGLLNIFILGLLGGLVGLVMPCTFPMIPLTV
                     SFFTKRSASRSKGIANAFLYGFFIFLIYVLVSVPFYFLQGNNANILNTISTNVWLNLF
                     FAAVFVAFALSFFGLFEITLPSGLSNSVDAKSDISSVGGIFFMALTLAIVSFSCTGPI
                     LGSFVVSSLGPNGGAIQLTVVLAGFGFALGLPFALFALFPSWLQSLPRSGGWMNTIKI
                     VFGFVELALALKYLSNADLVVHWGLLKRETFFALWILIGLALVLYLLGVIKFHHDPPP
                     KKLGGVRVTTAILFLAFVIYLIPGLTNTHYSNRALVSGFPPPLSYSLYGAAKAGGEVE
                     ANVVNDYDKALQLAKAQHKPILIDFTGWACVNCRKMEENVWPKPGVKELISKDYILVS
                     LYVDDRKQLPDDQQFVFTTSDGSKKSIRTVGDKFATLQSENFKNASQPLYVVISPDEK
                     LLNKPVGYTDNVTTYAQWLQCGLDAYKRMQ"
BASE COUNT          892 a          865 c          908 g          806 t
ORIGIN      
        1 gggggtgcgg gtaatgactt tttgctcgat cgcgatggct gggcagaggc ccttgatata
       61 atccacatcc ggcttattca tgcgggccat gaattgccgg gcgtaagcgc taaggctttc
      121 tgcgtatcta cgctggcctt ccgcaaaaag agtatcgatc gtcagtgaag atttacccga
      181 tccggagacc ccggtgacga ccaccagttt attcctgggg atcgctacat cgatattctt
      241 taaattatgt accctggctc cctttattaa aatggcgtcc actggtgcgg aagatttatt
      301 tactgttttg cccggctgtt tgacttttcc cgaagtgctg ttcatattat ataatagaca
      361 aacctacatc aaaatggttg ttaaatttgc ccggaagatc aggctgattg cgttatcggt
      421 cccaaaaata cccgtttcgt tggaatagga cgtattatag gacaaaatcg catacttagt
      481 acgttgattt taatggagaa tggggatttc accaacaaaa aatttcccaa aatgttgttt
      541 tttccaaaaa ttgtatatct ttaattcctg ttaggaattt atacacatat taacattccg
      601 aacaaaacaa accctggttt ttaccttaaa aatagttcgc ctatagattg aacacttcta
      661 catagaaaag ccaaaccatc tttaaaatcc ataatatata tcctatcacc tttaaaaccc
      721 gtagaagttt atgacattgc tacgcaaaaa aactgatcac gaacttattc accaattcag
      781 ggacggcgat ctgtacgcac tagaagcgct catcgtccgt cacaaggata agttgtacac
      841 ttccatcctc ttcctcgtta aggacaaata ccttgccgag gatatcttcc aggacgtttt
      901 aatcaaaatt atcgacacca ttcgcggcgg ccgctacacg gaagaaggaa aattccttcc
      961 atgggcgatg cgtatcgcgc acaacctgtg tgttgaccat ttccgcaaag taaagaggac
     1021 ccctgccatc aagacgagcg atgaccggga catcttcgaa gtgatccact ttacggaaga
     1081 aggcgccgac accaagatga tgaagcgcca gagccacgac cgggtacggc agatgctgga
     1141 cctgttgccg gaggaccagc gcgaagtgat catcctgcgt cactatgccg accttagctt
     1201 taaagagatc gcagccctga ccaactgcag catcaacacg gcgctgggcc ggatgcgtta
     1261 tggtctgatc aatcttcgta agatgatgac cgaaaaaaag attgccttgt aatgattttt
     1321 actctggaga aggatttgaa agatggtagt ccaatcgcag gtacgaagca atacgatggg
     1381 cgagtttttt aataatcttt aactactttt gggtacgggg aacggaaaca accgctaact
     1441 tcattgtgtg aaaagatcca ttaaaaaaaa tattgagagt tcccgcttga aaaagcgggg
     1501 actttttttg tttcagccgg ttcaggccgg agaaacttgt aacgcttgtc attctggccc
     1561 cccatctggt gtttaagcac ttgcccttag tcttgccgga aggccgggac agaatgacgc
     1621 ctaattaaag acgccgcctt ttaccgggcg gcgtctttct tttatggagc ctgattattt
     1681 attgcatccg tttgtaagcg tcgagcccgc actggagcca ttgggcatag gtcgtgacat
     1741 tgtccgtata ccccaccggt ttgttcagca gcttttcatc ggggctgatg acgacatata
     1801 atggctgcga agcattcttg aaattctccg attgcagggt ggcgaacttg tcgccgaccg
     1861 tgcggatgga tttcttgctc ccgtcgctgg tggtaaagac gaactgctga tcgtcgggca
     1921 gttgtttgcg gtcgtccaca tagagggaca ccaggatata gtccttgctg atcagctcct
     1981 ttacccccgg ttttggccag acattctcct ccatcttccg gcagttgaca caggcccagc
     2041 cggtgaagtc gataaggatg ggtttgtgct gcgctttggc cagttgcagg gccttgtcat
     2101 agtcattgac cacattggct tcgacctcgc cgcccgcctt cgcggcgcca taaaggctat
     2161 aggacaacgg cggcgggaaa ccgcttacca gggcccggtt ggaataatgg gtgttggtga
     2221 ggccgggaat aagatagatg acaaaggcca ggaacaggat cgccgtggtg acccttaccc
     2281 cgcccagctt ctttggcggc gggtcatggt ggaatttgat cacccccagc agatagagca
     2341 cgagcgccag gccgatgagg atccagagcg caaagaaagt ctcccttttc agcaagcccc
     2401 agtggacgac gaggtcggcg ttggacaaat atttaagcgc aagcgccagt tccacgaatc
     2461 caaacacaat tttgatggta ttcatccagc cccccgaacg gggcagcgat tgcagccagg
     2521 aagggaaaag ggcgaacagg gcaaagggca gacccagggc aaagccgaag ccggcgagga
     2581 caacggttaa ctgaatggcg ccgccattcg gaccgagaga gctgacgaca aagctgccga
     2641 gaatcggtcc cgtacaggag aacgagacga tggccagggt aagcgccata aagaagatgc
     2701 cgccaacact gctgatatcc gatttcgcat ccaccgaatt ggagagaccg ctgggcaggg
     2761 tgatctcgaa aagcccgaag aaagaaagcg caaaggctac gaacacggcc gcgaagaaca
     2821 ggttcagcca gacgttggtg gagatggtat tgaggatgtt ggcgttattc ccctgcagga
     2881 aatagaaggg gacgctgacc agcacataga tcaggaaaat aaagaaccca taaaggaagg
     2941 cgttggcgat ccccttcgac cgcgaagccg agcgcttggt gaaaaaggaa accgtgagag
     3001 ggatcatcgg gaaggtgcag ggcatgacga gaccgacaag gccgcccagc agtcccagga
     3061 taaagatgtt caacagtcct tttgtatcgc cgccggtgcc gccgcagcta ttgacgggat
     3121 tggccagatc gatggtactc ttttttaatc cgctcgaagc gctggccgac tcctgcagtc
     3181 ccgcagcctt cgccaccagg ccgccaccgg ctgtcacccc atagcggaag ggaatttcca
     3241 cgggacccag caccgagtcc ttatagatcg ccataaaggt gaccgtccct ttcaggtcac
     3301 cggcggcggc gcccgtgggc tgaagatgta cgagccactg tgcgtcgccg gtaaagaagc
     3361 gggtctccgc attgttaaag agcggctcct ttttacgctg gacggggctc ttttcttcga
     3421 tgccggtgat cttttccttt gcggaactat ccagcgctac ccgggaattg g
//
LOCUS       DATGFG010000445         5530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1179063, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000445 DATGFG010000000
VERSION     DATGFG010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179063"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..351)
                     /locus_tag="VKQ52_08255"
     CDS             complement(97..351)
                     /locus_tag="VKQ52_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87217.1"
                     /translation="MTPYIQNNQNYLAEEYRRFENYLLQESRIALLKIQSWQFAMKTP
                     EVGEHYQQEATDMVKKSLLSFVPNSFVLSEEGYYFKPIFN"
     gene            complement(815..2842)
                     /locus_tag="VKQ52_08260"
     CDS             complement(815..2842)
                     /locus_tag="VKQ52_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLZ87218.1"
                     /translation="MFINKLLFSTLFFAGMLAAVQDVRAQACTTAGQTPATAFPVCGT
                     SVFQQNSVPLCGGSNNIQIACATGPYTDVNPFYYKFTCFTAGTLGFLITPNTGSDDYD
                     WQLFDVTGHNPDDIYTNTSLFVSGNWSANSGATGAVAGVTGNVNCAGYSFPNKNGMPT
                     LIAGHNYLLLVSHFTLSQSGYALSFGGGTAGITDTVAPAVKAAAPVCDGSRIRVALNK
                     GMQCSTLAADGSDFSLSPAPGGLKVVGAYAVNCSGFDLDSLVVVVNGPLPPGNYSLTV
                     QTGTDGNTLLDVCGNQVPVGQAVSFTMVAPQPTPLDSLTPPGCAPDLLQLVFPKRIAC
                     SSIAANGSDFTVQGPTPVSVVGAYGQCDTGDMTYKILVKLSAPIQTAGNYRIVLAPGS
                     DGNTLIDECGQVSPSSSLPFTTGDTVSAALLTDQVILGCRTDTIIYSYPSVDGVNRWQ
                     WLFDGTDSSRLQDPPDRIYTVFGMKNVRLIVSNGFCTDTASLSLVLDNAFSAGLEVPN
                     ILCPKDYANYINKSTGTVNSWNWDFGDGTTYSGETPPDHLYPLTGVETKYTVTLVVGN
                     ANGCFDTAAQQIDVLRSCYIAVPSAFTPNGDGLNDYLYPLNAYKADNLTFKVFNRFGQ
                     LVFESHEWTQKWDGTVHGHAEPAGTYVWMLEYTDRDTKKKVFQKGTALLIR"
     gene            3322..>5530
                     /locus_tag="VKQ52_08265"
     CDS             3322..>5530
                     /locus_tag="VKQ52_08265"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HLZ87219.1"
                     /translation="MDKLIYVVPVLGAVGLLFTFIKFAWVSKQDPGNARMQEISKHIA
                     EGAMAFLKAEWKILSYFVIIAAILLALMGYSNPDSHWSIAVAFILGAVLSATAGYIGM
                     RSATKANVRTAHAARTSLSQALKVSFNGGSAMGFGVAGLAVMGLGGLFIILKAIFAPD
                     APVNSEEMRRTIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGQETVADDKFGGMSPILLP
                     MLIAGIGIIFSIIGTFFVRISDSAGVNTHTVQKALNMGNWGSIILTALACIGLVYYVL
                     PETMTLRGTDFTKWGVLGAIGVGLLVGTLMSIITEYYTAMGKAPVLSIIRQSGTGHAT
                     NVIGGLAVGMQSTFLPILVLAGGIWGSFACAGLYGVAIAAAGMMATTAMQLAIDAFGP
                     IADNAGGIAEMSELPKEVREKTDVLDAVGNTTAATGKGFAIASAALTALALFAAFVGV
                     AGIQGIDIYKAKVLASLFVGGMIPFIFSSLAIQAVGQAAMAMVEEVRRQFRTIPGIME
                     GTGIPEYDKCVAISTQASIKKMILPGAIAILFPLIIGFLLGPEALGGFLAGATVCGVL
                     MGIFQNNAGGAWDNAKKSFEKGVEINGEIYYKKSEPHKASVTGDTVGDPFKDTSGPSM
                     NILIKLMSIVSLVIAPTLASLFHTHDEGALHRAPKMEKQAVVSTGSPKTTATPL"
BASE COUNT         1241 a         1545 c         1483 g         1261 t
ORIGIN      
        1 accgagaaag agctgaagga atgctacgat ccgacaatag cataaaaaaa gaggccatcc
       61 ggcctctttt cttttatagg tgtagcaagc gatcgattag ttgaagatcg gtttgaaata
      121 atatccttcc tcgctgagga caaaactgtt ggggacgaag ctcaaaaggg atttctttac
      181 catgtcggtg gcttcctgtt gataatgttc tcctacttca ggagttttca tggcgaactg
      241 ccagctctgg atctttaaaa gggcgatacg gctttcctgc agaagatagt tctcaaaacg
      301 acggtactct tcggccaggt aattctgatt gttctgtatg tacggggtca tagggatcac
      361 tattagtttt aaaagaatat tggattgtgt tgcggacgaa cccgggagcc gcttctaaga
      421 ctaaacgcag gttcgggtgt gaaattgtgc ttttttttcg gtttattcct tcccccggaa
      481 tttttttccc aacccgggaa gattttggct ggacgcggaa tcacacaata cttattttca
      541 atattttatg atttggcacc cgattagtat tatatagatc aaacagctct tacaagatat
      601 cggttcaact caagatatta ccttacaaac gtgttgacgg ccatcaatag tttaaaaggt
      661 ccctaggttt ttagtttttt caggcttaca ggttcttcta caaaaaagtg ctcggccccg
      721 ctccagaaaa gtggggcctt cgcattttta tctatatgtc ggggaagcgc tccgcgcttc
      781 cggatgagag tccccgctac gcgggggctt ttttttatct tattaataag gcggtgcctt
      841 tctggaagac ctttttcttt gtatcccggt cggtatattc cagcatccat acataggttc
      901 cagcaggctc ggcatgacca tgcacggtgc catcccattt ctgcgtccat tcatgggact
      961 cgaataccag ttggccaaag cggttaaaga ccttgaaggt caggttatcc gccttgtagg
     1021 cgttcagggg atagagatag tcgttcagcc cgtcgccgtt cggggtaaag gcgctgggaa
     1081 cggcaatata acagctccgc aaaacatcga tctgctgcgc ggccgtatca aagcagccat
     1141 tggcattgcc gacgacaagg gtaacggtat attttgtttc cacacccgtc aggggataga
     1201 ggtgatcggg cggtgtctcg ccgctgtacg tggtgccatc gccaaaatcc cagttccagg
     1261 aattgaccgt acccgtgctt ttatttatat aattcgcgta atccttcgga caaaggatat
     1321 tggggacttc caggcccgcg ctgaaggcgt tatccaacac aagcgacagg cttgccgtat
     1381 ccgtacaaaa cccgttcgag acgatcaacc gcacgttctt catgccaaag accgtgtaga
     1441 tcctgtcggg cgggtcctgc agccggctgg aatccgtgcc gtcaaaaagc cattgccacc
     1501 ggttgacccc atcgacggat ggatagctat agatgatggt atccgtcctg caccccagga
     1561 ttacctgatc ggtcagcagc gccgccgata cggtatcgcc ggtggtaaac ggcaggctcg
     1621 acgatggact cacctgtccg cattcatcga taagcgtatt gccgtcggag ccgggagcca
     1681 ggacgatgcg gtagttgccg gccgtttgga tcggcgcgga caatttgacc agtattttat
     1741 aggtcatatc ccctgtatcg cactgcccat atgctccaac cacggaaacg ggcgtggggc
     1801 cctgcacggt aaagtccgag ccattggcgg cgatggagga acaggcgatc cttttgggga
     1861 agaccagctg cagcagatcc ggtgcacagc cgggtggggt aaggctgtcc agcggcgtcg
     1921 gttgcggcgc caccatcgtg aacgagaccg cctgccccac cggaacctga ttcccgcaga
     1981 catcgagcag ggtattacca tccgtacccg tctgcaccgt cagcgaatag ttgcccggcg
     2041 gcaaggggcc attcacgacg acgacaaggg aatccaggtc gaagccgctg cagttgacgg
     2101 cataggcacc gaccaccttt aacccgccgg gcgcgggcga caaactaaaa tcgctgccgt
     2161 cggcggcaag ggtggaacat tgcatccctt tgttcagggc tacccgtatc cggctgccgt
     2221 cacaaaccgg ggctgccgcc ttcacggcag gcgcgaccgt atcggtgatg cccgccgtac
     2281 cgccgccaaa ggagagggca tagccgcttt gcgatagcgt gaaatggctc accagcaata
     2341 agtaattatg tccggcgatc agcgtcggca tgccattctt gttcgggaaa gaatatcccg
     2401 cgcaattgac attcccggtg accccggcta ccgcgcccgt cgctccggaa ttggcagacc
     2461 agttgccgct gacgaagagg ctggtgtttg tatagatatc atcggggtta tgaccggtaa
     2521 catcgaaaag ctgccagtca taatcatccg aaccggtgtt cggagtaatg agaaatccca
     2581 gggtgccggc ggtgaagcag gtgaacttat aataaaaagg gttgacatcg gtataggggc
     2641 cggttgcaca ggctatctga atattgttgc tgccgccgca aaggggcacg ctgttctgct
     2701 gaaaaacaga tgtcccgcaa acaggaaagg cggtggccgg cgtctggccg gcggtagtac
     2761 aggcctgtgc ccttacgtcc tgcactgcag ccagcattcc ggcaaaaaat aaggtcgaaa
     2821 aaagcagttt gtttatgaac atgcacccat agagttattc agcaagacga tggtatccgc
     2881 attacggttg tctcccatcc ccgcttttgc cccgttaaac agcaaaaatt tgtccatttc
     2941 gggagtaatc ttccaaaaac gggaagaatt aaccgagtgg taaatatata aaaaaatgac
     3001 tttaaacacc ttgcgcactg gcatagtatt cgaataaacg attataccca aatagacacc
     3061 cctttacccc ttggttcttg acctaaacaa cctgagcaga aaaccttgaa caaggaaagg
     3121 tcccccagag gcccgatccc gtagtagtag aattctcatt caagttccgg tttttacctg
     3181 aagaatcctt tcgaactgaa gcgaaaggat ttttcattat gggcaatatt tgacattttc
     3241 cccctatttt tgccgtccct taagccatcc gcaataagta aacgattgcc gggatgaaaa
     3301 tgctgtttaa ggtcaaaact tatggacaaa ttgatttatg tagttcccgt tttgggggcc
     3361 gttggcctcc tattcacatt tattaagttt gcctgggtgt cgaaacagga tccgggcaat
     3421 gcccggatgc aggagatcag caagcacatc gccgaaggcg ccatggcctt cctgaaagcg
     3481 gaatggaaga tcctctccta ttttgtcatc atagcggcca tcctcctggc cctgatggga
     3541 tactccaatc ccgattcgca ctggtccatt gccgttgcct ttatccttgg cgccgtgctt
     3601 agcgccacag caggctatat cgggatgcgc agcgccacca aggccaatgt ccgtaccgcc
     3661 catgcggcac gcaccagcct tagccaggca ttaaaggtat ccttcaacgg tggttccgcg
     3721 atgggttttg gcgtcgccgg tctcgccgtt atgggcctgg gcggtctctt tatcatactg
     3781 aaagccatct tcgcccccga cgcccctgtc aactccgagg aaatgagacg taccatcgaa
     3841 gtgctgaccg gtttttcgct gggcgccgaa tccatcgcgc tctttgcgcg tgtcggcggc
     3901 ggtatctata caaaggcggc cgacgtcggc gccgacctcg tcggtaaagt ggaagccggc
     3961 atccccgagg acgacccccg caaccccgcg accatcgccg ataacgtagg cgacaatgtc
     4021 ggtgacgtgg ccgggatggg cgccgatctc ttcggcagct atgtggccac cgtactcgct
     4081 acgatggtat tgggacagga gaccgtagcc gatgacaaat tcggcgggat gtcccccatc
     4141 ctccttccca tgctcatcgc gggtatcggg atcatcttca gcatcatcgg aacgttcttc
     4201 gtccggatca gcgattcggc gggggtcaac acccatacgg ttcaaaaggc gctcaatatg
     4261 ggcaactggg gctccatcat cctgacggcc ctggcctgta tcggccttgt ttattatgtt
     4321 ctccccgaaa cgatgaccct ccggggtacc gacttcacca aatggggtgt gctcggcgcc
     4381 atcggcgtcg gtctgctggt cggaacgctg atgagcatta ttacggaata ctatacggcc
     4441 atgggtaagg cgcccgtact gagcatcatc cgccagtccg gcaccggtca cgcaacaaac
     4501 gtcatcggcg gtcttgccgt cgggatgcag tccaccttcc tgccgatcct cgtactggcc
     4561 ggcggcatct ggggttcctt cgcctgtgcc ggactctatg gggtcgctat cgccgccgcc
     4621 gggatgatgg ctaccacggc catgcagctg gctatcgacg cctttggccc catcgccgat
     4681 aacgcgggcg gcatcgccga aatgagcgaa ctgcccaagg aagtccgcga gaagaccgac
     4741 gtgctggacg ccgtaggaaa tacgacggcc gccacgggta aaggattcgc catcgcttct
     4801 gcggccctga ccgccctggc gctcttcgcc gccttcgtcg gtgttgccgg tatccagggt
     4861 atcgatatct ataaggcaaa ggtcctggcc agcctcttcg tgggcggcat gatccccttc
     4921 atcttttcct ccctggcgat ccaggccgtt ggccaggcgg ccatggcgat ggtggaagaa
     4981 gtaaggcgcc agttccggac catccccggc atcatggaag gcacggggat acccgaatat
     5041 gataaatgtg tcgccatctc gacacaggcc tccatcaaga aaatgatcct cccgggagcc
     5101 atcgctatcc tgttcccgct gatcatcggt ttcctcctcg ggcccgaggc cctcggcggc
     5161 ttccttgccg gcgccaccgt ctgcggcgta ctgatgggta tcttccagaa caacgccggc
     5221 ggcgcctggg acaatgccaa gaaatcgttt gagaaaggcg tcgagatcaa tggcgagatc
     5281 tactacaaga aatccgagcc ccataaggct tccgtcaccg gggataccgt gggtgacccg
     5341 ttcaaggaca cctccggccc gtcgatgaac atcctgatca aactcatgtc catcgtttcc
     5401 ctggtcatcg cccccaccct ggcttccctc ttccataccc acgatgaggg cgccctgcac
     5461 cgggctccaa aaatggagaa acaggccgtc gtcagcaccg ggtcgccaaa gaccacggcg
     5521 acacccctgc
//
LOCUS       DATGFG010000446         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1686614, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000446 DATGFG010000000
VERSION     DATGFG010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686614"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VKQ52_08270"
     CDS             <1..291
                     /locus_tag="VKQ52_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87220.1"
                     /translation="EYVNACRMRTAIMRKVDPFISQYDIIIAPPETGDQLAITNLTGH
                     PSVTLPVGYHADGTPGSISFIGQLYGEAELLAFAKFFQDATSYNKQRPKGFE"
     gene            433..>2574
                     /locus_tag="VKQ52_08275"
     CDS             433..>2574
                     /locus_tag="VKQ52_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87221.1"
                     /translation="MRMLHNYLRLAMRNLTRNRIYSMINIAGLAVGLAVFWLMSLYIA
                     DESGYDRDSVNADRIYRVVHSGEWAGGRFRMAQTPPAFGPALAKEDPAIETAARVNAE
                     GGGTIVYGDKKLDVGDMLLADSSLLNVFAFPFVAGDASTALSAPNSIVLTRTLAAKLF
                     GSADDALHKTVTIQNEGAVEVTGVIEDRPINSHLRFSALRPLHETNEEWKNSYLYTYL
                     LLKKGVDIHSVEARIPGFCDRHIQPLLGKGASYRMELQAVTSIHLHSHLDFEMTRNGD
                     IGYVYLFAAVALLVLGIAVINYINLATARASVRMKEIGVRRVIGGSRKQLISLFLTES
                     VFFTLLSATIAGVLVWMALPAFDQLSGKALTIGQFGMAREIVLPALSAVVIGLAGGLY
                     PALFLSGFATIPALKGQVGGQSATVLFRKSLVTFQFVITIFLITGSAIIYLQLQFMQG
                     KDLGFNKDQTLTFHLPNPDVRRHIEDLKTQLLGNPSVEAAAAAGNPIGNNDIGERQIQ
                     WEQNGVIQSEPRKAQSFYIDADYLSTMQIGLAAGRNFSATRPMDVTGSVLVNETLVRE
                     MGYTDVNAIGKRVKLGTDANGQVMMASVIGVVKDFNIYSLQHKIEPLLLRMPPVWNEE
                     DNLYVRVRKGRIPEALKYIAGVYSRFDPSTTPDFHFLDDNFSQQYAAEKKQASILLIF
                     TVLAIFIACLGLLGLVTFSVGQRTKEIGIRKV"
BASE COUNT          541 a          615 c          818 g          600 t
ORIGIN      
        1 gagtatgtga atgcgtgccg gatgaggacg gccattatgc ggaaggtgga tccgtttatc
       61 agccagtatg atattatcat tgcgccgccc gagacggggg atcagttggc gatcaccaat
      121 ctgacggggc atccgtcggt gacgttaccg gtggggtatc atgcggatgg gacgccgggg
      181 agtatttcgt ttatcgggca gttgtatggg gaggcggagt tgctggcgtt tgcaaagttc
      241 ttccaggatg cgacctcgta taataagcag cggccgaagg ggtttgagtg atcatgctct
      301 gtgcgctttt cgcgacatgt atcgaaagcg gacagcaggt gtattgaaag tggactggat
      361 gggtgggggg atggaggagt aagtctctac gggacagcta tttgtgtaaa ttggcatgaa
      421 ttaggaacaa gtatgcgtat gctacacaac tatctccgtc ttgcgatgcg gaacctcacc
      481 aggaacagga tctactcgat gatcaatatt gccgggcttg ctgttggcct ggcggtgttc
      541 tggttgatgt cgctgtatat cgccgatgag tcgggttatg accgggattc ggtgaacgcg
      601 gaccgcattt accgggtggt acattcgggg gaatgggcgg ggggacgatt ccggatggcg
      661 cagaccccgc cggcttttgg gccggccctt gcgaaggagg acccggccat cgagacagcg
      721 gcgagggtca atgcagaagg cgggggaacg atcgtttatg gggacaagaa gctggatgtg
      781 ggggatatgt tgctggcgga cagttcgctg ctgaacgtat ttgcgttccc ctttgttgcg
      841 ggagatgcga gtaccgcgct gtcggcgccg aattcgatcg tgctgacgag gacccttgcg
      901 gcgaagttgt ttggcagtgc cgatgatgcc cttcataaga cggtcacgat ccaaaacgaa
      961 ggcgccgtag aagttacggg cgtgatcgaa gaccggccca tcaacagcca tcttcggttc
     1021 agcgcgctgc ggcccctgca tgagaccaat gaagaatgga agaattccta cctctatacc
     1081 tatttgctgt tgaagaaagg ggtcgatatc cactccgtgg aagcgcggat cccaggcttt
     1141 tgcgacaggc atatccaacc gcttctggga aagggcgcca gttaccggat ggagttgcag
     1201 gccgtgacct cgatccacct gcattcccat ctcgatttcg agatgacccg caacggcgat
     1261 atcggctatg tctatctttt tgcggcggtg gccttgctgg tgctgggtat cgccgttatc
     1321 aactatatca atctggccac ggcgcgggcc tcggtccgga tgaaggagat cggggtgcgg
     1381 agggtgatcg gtgggagcag gaagcagctg atcagtttat tccttaccga atcggtattc
     1441 tttaccctgt tatctgcaac gatagccgga gtcctcgtct ggatggcgtt gccggccttt
     1501 gaccagttgt ccgggaaggc gctgacgatc gggcagttcg ggatggcccg ggagatcgtg
     1561 ctgccggcct tatcggctgt tgtgatcgga ctggcagggg gcctctaccc tgccctgttc
     1621 ctgtcgggtt ttgctaccat ccccgccctg aagggacagg tggggggcca gtcggcgacg
     1681 gtgttgttcc gcaaatcgct ggtgaccttc caatttgtca tcactatctt tcttatcacc
     1741 ggttcggcga tcatctattt gcaattgcag tttatgcagg gaaaggatct cggcttcaac
     1801 aaggaccaga ccctgacctt tcatctcccc aacccggatg tgcgccggca tatcgaggac
     1861 ctcaagacgc agttgttggg caatccttcg gtagaagcgg ccgctgccgc gggaaacccg
     1921 atcggcaata acgatatcgg cgagcgccag atccaatggg aacagaacgg cgtcatacag
     1981 tccgagccaa gaaaggcaca atccttttat atcgatgccg attatctttc cacgatgcag
     2041 atcggcctgg cggccggaag aaatttctct gcgacgcggc cgatggatgt caccgggtcg
     2101 gtactggtga atgaaacgct ggtgagagaa atgggataca ccgacgtcaa tgccatcggc
     2161 aaacgggtaa agctgggaac ggatgccaac gggcaggtga tgatggcttc ggtgatcggg
     2221 gtggtaaagg acttcaatat ttattccctc cagcacaaga tcgagccgtt gttattgcga
     2281 atgccgccgg tgtggaatga ggaagataat ctttatgtcc gcgtgcggaa ggggcggata
     2341 ccggaggcat tgaaatatat tgccggggtg tacagccgtt tcgacccttc cacgacaccg
     2401 gactttcatt ttttagacga taatttctcg cagcaatatg cagcggagaa aaagcaggcc
     2461 agtatcctgc tcatctttac ggtattagcc atctttatcg cctgtctggg gttgcttggc
     2521 ctggtgacat tcagcgtggg gcagcggacg aaggagatcg gtatccggaa ggtg
//
LOCUS       DATGFG010000447         4995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_46083, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000447 DATGFG010000000
VERSION     DATGFG010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46083"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2238
                     /locus_tag="VKQ52_08280"
     CDS             <1..2238
                     /locus_tag="VKQ52_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ87222.1"
                     /translation="TDSGFHPFTVAAVAEDMPLNTSISFDVLGNYQYLEMTDTDRVKS
                     SANWGLTYGDETYLLLRPGSRLPQQTRSLQAFRQQHFPEEAGGFKAADLGHKEAEEVK
                     NEAKGEQKEGEGGPKVGEGGQKAAKGGLKEGEGGQKVAEGEPKTGGGGQKVGEVEPKA
                     GEGGQNLAKGEQKEGKGDQKATKGEQKAAESRPRPAKVQARYVLQPLLSIHTDTSVQG
                     ASPTIDAKNIWILIGIAAGIVLIASINFTTLAIARSAGRAREVGVRKVVGGLRRQLIG
                     QFLTESVLLSILSAVIGYILALALLPWFNQLAGKHLAFSFRIFPQLSWMLVGLTLLVG
                     LLAGFYPALVLSGFNTIEVMKAKIKLGGSNLFTRSLVTFQFVLSIGLIIATVVMVRQV
                     NFMRSKDLGLVKENTVLIHAGNIDAATAYPLFRQELAGNPSVRGVAASGIGLGEGEGY
                     YGGGYDFNGKRTGVIEYPVDENFIPVMGVRLVAGRNFDRGITSDTAGNIIVNETLVHD
                     DLGITPRQAIGQQIKTASRPRSNPRPEVKTIIGVVRDFNFERLNKKVRPQLFSMPANF
                     TPSVFFIHLGAGDPTQALDQMAAVWRRLSPDIPFSYSFLDENLDRFYKSEARWGNIIA
                     CAGGISIFLACLGLFGLAALSAANRVKEIGIRRILGASTAAIVGLLTGGFLRLVVIAA
                     VIASPIAWVIMNRWLRDFAYRIDVGWWVFAVTALAAMGIAFLTIGLQAFRAARINPVE
                     NLRVE"
     gene            complement(2259..3545)
                     /locus_tag="VKQ52_08285"
     CDS             complement(2259..3545)
                     /locus_tag="VKQ52_08285"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HLZ87223.1"
                     /translation="MDYAQTVDYLLTKLPMYSRIGAAAYRKDLTNTWQLSDFVGNPER
                     RFRSIHIAGTNGKGSTSHMLAAIFQEAGYKTGLYTSPHLKDFRERIRINGEMIKETAV
                     VDFIQRIRPISEQLDPSFFEISVVMAFDYFAQEQVDIAIVEVGLGGRLDSTNIITPEL
                     SVITNIGYDHMNLLGDTLAAIAFEKAGIIKKGVPVVVGEHQPETAPIFSQRAHEEEAP
                     IRFADQHRYITDWKYQRHTLVAEVATSPVANDVNFYTLDLPGLYQAKNLVTVLEAVHV
                     MTGKGWKLDQKTVIRALRQVKKLTGLHGRWEMVQEHPDVVLDVAHNEDGIRQLLRQIE
                     VTDHEELHIVLGMVKDKAIDKVLSLLPRQARYYFTRAQIPRALPEAELAAQANAVGLP
                     GNAYPTVSAALAAAKAHARPRDLVVVCGSVFVVAEV"
     gene            complement(3589..4473)
                     /locus_tag="VKQ52_08290"
     CDS             complement(3589..4473)
                     /locus_tag="VKQ52_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87224.1"
                     /translation="MTERPDSFESEKNLKAGGYTAVICVLLLLVLIYAGWTQPVEPPP
                     VAEEGIEVNLGNSDKGLGDNQPYLPGQPSAEDKEKYTPPRQAVVEKEPVKDVETDDNN
                     KEDAPVVKKAIVTKPNATKIPDKEITRTKTKPVKQPETLPEKPAPKPKAVFHGVNGNG
                     TGGNAADDFKPGGNQGIAGGRGDQGAPGGDPKSNNYTGGGHGSGGVAISRGLGGRHIA
                     STPSFTDDFNENAKVAVDITVDAAGNVTGAQYTPRGSTTSNANYKEIAERKARQVKFT
                     PGSDESTGTIVFNFKVHA"
BASE COUNT          926 a         1345 c         1614 g         1110 t
ORIGIN      
        1 acggacagcg gtttccatcc cttcacggtg gcggcggtag ccgaggacat gcccctcaat
       61 accagcatct ccttcgacgt actcggcaac tatcaatacc tcgaaatgac ggacaccgat
      121 cgcgtaaagt cctcggccaa ctgggggctt acttatggcg atgagaccta tctgctcctc
      181 cgtcccggga gccggttgcc gcagcaaacc aggagcctgc aggccttccg gcagcagcat
      241 ttccccgagg aggcgggtgg ctttaaggcg gcggacctcg gccataaaga ggcagaagag
      301 gtaaagaatg aggcgaaagg tgagcagaag gaaggtgagg gtgggccgaa ggtaggcgaa
      361 ggtgggcaga aggcggcgaa gggtgggttg aaagaaggcg aaggcgggca gaaagtggca
      421 gaaggtgagc cgaagacagg tggaggcggg caaaaggtgg gtgaagttga gccgaaggct
      481 ggagaaggcg ggcagaattt ggcgaaaggg gagcagaagg aaggcaaggg tgaccagaag
      541 gcgacgaaag gggagcagaa ggccgccgaa tccaggccca ggccggcaaa ggtccaggcc
      601 cggtacgtgc tgcagcccct gctaagcatc cacaccgata cgtcggttca gggggctagt
      661 cctaccatcg atgcgaagaa tatctggatc cttatcggca tcgcggcggg tatcgtcctc
      721 atcgcttcga tcaacttcac tacgctggcg atagcccgta gcgcaggtcg cgcccgcgaa
      781 gtgggcgtgc gtaaggtcgt aggcggattg cggcggcaac tcattggaca attccttacg
      841 gagtcggtgt tgcttagtat cctgtccgct gttatcggtt atatattggc gttggcgttg
      901 ctgccctggt tcaatcaatt ggcgggaaag cacctggcgt tctcgtttcg gatcttccca
      961 caattgagct ggatgctggt ggggctgacg ctgctggtcg ggttgctcgc gggcttttat
     1021 cccgcgcttg tcctatccgg gtttaacacg atcgaggtga tgaaggcaaa gataaaactg
     1081 ggcggctcca acttatttac gcgtagtctc gtcaccttcc agttcgttct ttccatcggc
     1141 ctgatcatcg caacggtggt catggtccgg caggtgaatt ttatgcgcag caaggacctc
     1201 gggctggtaa aggagaacac tgtccttata catgcgggca atatcgacgc ggccaccgcc
     1261 tatccgctat tccggcagga actggccggc aacccctccg ttagaggcgt tgcggcctcg
     1321 ggtatcgggc tcggggaggg ggagggatat tatggcggcg gctatgactt caacgggaaa
     1381 cggacgggtg tgatcgaata tcccgtagat gagaatttta tcccggtgat gggagtgcgt
     1441 ctcgtggccg gccggaattt cgatcgtggc atcacctccg atacggccgg caatatcatc
     1501 gtcaacgaaa ccctggttca tgacgatctg ggtataacgc cccggcaggc catcgggcag
     1561 caaataaaga cggcgtcgcg gccgcggagc aatccccggc cggaggtgaa gaccatcatc
     1621 ggcgtggtgc gggatttcaa tttcgaacgg ctcaataaaa aagtgcggcc gcagctattc
     1681 tccatgccgg ccaatttcac gccctctgtt ttcttcatcc atctcggggc cggggacccg
     1741 acgcaggcgc tggatcagat ggcggccgtc tggcggcggc ttagcccgga tatcccgttt
     1801 agttacagct ttctcgacga gaacctcgat cgcttttata aatccgaggc ccgctggggg
     1861 aatatcattg cctgtgccgg tggcatctcg atcttccttg cctgtctggg tctcttcggc
     1921 ctggcggccc tgtcggcggc caatcgggtg aaggagatcg gtatccgcag gatccttggc
     1981 gcctctactg ccgccatcgt cggcctgctg acgggcggat ttctgcgcct ggtggtgatc
     2041 gccgcggtga tcgcttcgcc cattgcgtgg gtgatcatga accggtggct gcgggatttt
     2101 gcctatcgta tcgatgtggg gtggtgggta tttgccgtga cggccctcgc ggccatgggg
     2161 atcgctttcc taacgatcgg gctccaggca tttcgcgccg cccgtatcaa cccggtagag
     2221 aacctgcgcg tggagtgatc gccgcgagtc tcgtagcctt atacctccgc caccacgaac
     2281 acgctgccgc acactacgac cagatcgcgg gggcgtgcgt gtgccttcgc cgccgccagc
     2341 gccgcactga cggtagggta ggcgttgccg ggcagcccga ccgcattggc ctgcgccgcc
     2401 agttcggctt cggggagcgc gcgggggatc tgtgcacggg taaaataata gcgcgcctgc
     2461 cggggcagga ggctcagcac cttgtcgatg gccttgtcct ttaccatccc cagcacgata
     2521 tgcagttctt catgatctgt cacctctatc tgccgtagca actggcggat gccgtcctcg
     2581 ttatgggcga catcgaggac gacgtcgggg tgttcctgca ccatctccca gcggccatgc
     2641 aaccccgtca gcttcttcac ctgccgcagc gcgcgtatca ccgtcttttg atccagcttc
     2701 cagcctttgc cggtcatgac atgtacggcc tcgagcacgg tgacgaggtt cttcgcctgg
     2761 tagagacccg gcagatcgag agtataaaaa ttgacgtcgt tggccacggg agaggtggct
     2821 acttccgcca ccagtgtatg tcgctggtat ttccagtcgg tgatatagcg gtgttggtcg
     2881 gcgaagcgga tgggcgcctc ttcctcgtgc gcccgctggc tgaagatggg cgccgtttca
     2941 ggctggtgtt cccccacgac gacgggcacg ccttttttga tgatgcccgc cttctcgaag
     3001 gcgatggctg cgagtgtatc gcccagcagg ttcatatggt cgtatccgat attggtgatg
     3061 acggacaatt cgggggtgat gatattcgtg ctgtcgagcc ggccgccgag gcctacttcc
     3121 acgatggcga tgtccacctg ctcctgtgcg aaatagtcga aggccataac gacgctgatc
     3181 tcgaaaaagg acgggtccag ctgttcggag atcgggcgga tgcgctggat aaaatcaacg
     3241 acggctgtct ctttgatcat ttccccgttg atgcggatgc gttcccggaa atccttcagg
     3301 tggggggagg tataaagacc ggttttgtaa cctgcttcct ggaagatggc cgccagcata
     3361 tggctcgtgg agcccttgcc gttggtaccg gcgatatgga tgctgcggaa gcggcgttcg
     3421 ggattgccca caaaatcgct cagttgccag gtgttggtaa gatccttccg gtaggcggct
     3481 gccccgatgc ggctatacat ggggagcttc gtcaaaagat agtcgaccgt ttgtgcgtag
     3541 tccataggag ggcaaagata gagaaattgc cgggtcgcac ttggacggtt aagcgtgcac
     3601 tttgaaattg aaaacgattg tgcccgtgga ttcatcgctg cccggggtga atttcacctg
     3661 cctggccttg cgttcggcga tctccttgta attcgcatta cttgtggtcg atccgcgcgg
     3721 ggtatattgg gcgccggtga cattgccggc cgcatcgacc gtgatgtcca ccgcaacctt
     3781 cgcattctcg ttaaagtcgt cggtgaagga cggtgtgctg gcaatatgcc ggccaccaag
     3841 tccgcgggag atggcgactc cgccgctgcc gtgaccgccg ccggtgtagt tgttggattt
     3901 ggggtcgccg ccgggtgcgc cctggtcgcc gcggccgccc gcgataccct ggttaccgcc
     3961 gggcttaaag tcatcggctg cattgccccc cgttccatta ccgttaacgc cgtggaaaac
     4021 ggctttgggt tttggcgcgg gtttctcggg aagagtctcc ggctgtttga ccggttttgt
     4081 cttcgtcctg gtgatctctt tatcggggat cttagtggcg ttgggcttgg tgacgatggc
     4141 cttcttcacc accggtgcat cctctttatt attgtcgtcg gtctccacat cttttaccgg
     4201 ttccttttcg acaacggctt gtctgggagg tgtatacttc tccttgtctt cggccgaagg
     4261 ttgtcccggc aggtaaggct ggttgtcgcc gaggccctta tcgctgttgc ccaggttgac
     4321 ctcgatgccc tcttcggcca cggggggtgg ctccaccggc tgtgtccaac cggcatagat
     4381 gagcaccagc agcaggagca cgcaaatgac tgcggtgtag ccaccggctt tgaggttttt
     4441 ttcggattcg aagctatcgg gcctttcggt cataggagaa atttcaactg caatttacta
     4501 aaaccggatg gaatattccg ggggataacg cagtaaaatg ggagagaatt gccccgttgc
     4561 cccctgccaa aatgttaaaa atgtggaaga tgggcgatac ggcgcagcgg agtgcgtcaa
     4621 aacgggcggt acagcgacca ggggccgtag gtgcggagtc cggggggctg taggtgcggc
     4681 gtccagtggg ccatagagtc cgcgaccagg gagccattgg tgttgcgacc agggagccat
     4741 aggtaacgcg atggtggcaa gtggcaaccc cggtgtccac cctccggtgg cccgggaggg
     4801 agattgggct agttgtctcc ggtttccagg ggagaggctg tgttagtacg cactccggtt
     4861 cccaagggag aggctgtgtt ggcatgctct ccggtttcca ggggagaggc gagctagtgc
     4921 gcactccggt gcccgttgtg acgtctgtgg cggcggtact tcatcgggta atggatgtcg
     4981 taggccattt cgtag
//
LOCUS       DATGFG010000448         4438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1389490, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000448 DATGFG010000000
VERSION     DATGFG010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389490"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VKQ52_08295"
     CDS             complement(<1..207)
                     /locus_tag="VKQ52_08295"
                     /inference="COORDINATES: protein motif:HMM:NF038404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 2-containing transporter"
                     /protein_id="HLZ87225.1"
                     /translation="MNARPPKWPLLFLRWFCREEYLEEIEGDLTELFLRRHAHSPAKA
                     RRQFLWNVLKHVRPEFVKPVKLRKP"
     gene            complement(204..533)
                     /locus_tag="VKQ52_08300"
     CDS             complement(204..533)
                     /locus_tag="VKQ52_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLZ87226.1"
                     /translation="MKHHSIGEFEELVLLLVAALHDEAYGVSIQENLLERLSKNINIS
                     AIHVALKRMEDKGFVKSRFGGITEDRGGRRKKYYVITPLGKRILDGQYELRTGLYKMI
                     PKISFGR"
     gene            complement(586..1548)
                     /locus_tag="VKQ52_08305"
     CDS             complement(586..1548)
                     /locus_tag="VKQ52_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ87227.1"
                     /translation="MKTMSKTLYAALILSVTTVLFIGKAPAMQAPNGTAKIPRPASPP
                     TGFKHQYAVVNSVKIHYVIGGNGEPLLLVHGFGQNWYMWNRLLPELSKHFTVIAPDLR
                     GVGESGKPAGGYDKKNMAVDMHELMTKLGYQHINIAGHDIGMIVAYAYAAQFPGDVKK
                     IAFMDALLPGIEPVYSEVKAQAWWFGFFAQPHSGEIVSGNVGLFFTDFWPPLGYQKSA
                     FTQTEKNEFIRAYSVPGATTSAFHWFGEFPQDAIDDAAFMKNKLPMPLLAMGSEHFAG
                     SFLADHSRLVANEVHEVIIKDSGHWVVQEQTAQVQKALLDFFLN"
     gene            complement(1645..3687)
                     /locus_tag="VKQ52_08310"
     CDS             complement(1645..3687)
                     /locus_tag="VKQ52_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLZ87228.1"
                     /translation="MKNILWLLLFPLVSYAQKYQIYPGEYDNWVRHPKTKLLFDIVSS
                     STESVAGLPAIADGDGKGSASLHTAGFIGVDGYVYVWGDNACNISGLGLKLPWIGMTK
                     TAVSNARQVVAYANSGDGSGQGLGYGFAIVTNDNKLTLLGNTQSGFRGDGTEGNKAEP
                     GPYRVTVIGKPVQKVAVGSFIYVLYKDGTVDSWGGTRRKYYPTLVLGRGVDNPDPTRP
                     GAMKFPEPIVDLVGGGEWTYFKGRSGAIYGVAYNPRYLGLGANVSARNTPFNLTKTLG
                     LPGNPVQLAAGSQASYALMSNGDAYAWGDNTQAAIGNGKEAQFANFIAPWGGGLLWVD
                     KPVKINPAGVSFVKLFTGIGDAFYVVAEDNNGHLWVWGRNKGHVLWNGQGSPDGNIQA
                     TQPNRWDIPTPTKIDGFDRNNGQASGQGSGGEKTGDGDILVDVGGYKLHFVISKGKGI
                     PILFEAGGGEDATTWNKILLPIAKRTGTTLITYDRAGFGQSTFDTTQRGLMNGILGLE
                     MGLQKLGYDNNIMLVAHSQGGLYAQLYAARHPEKVKAAVLIDVTTTCFYDEKRLAATQ
                     RMINQQNTDKVKASDPGRYYQGADFSDNIESMRNVAFPNTIPVTDFVSDHPPFQDSTD
                     INDWKRCHREFAAASSNRTGILATGCGHFIFNDDPALVINAIVKAYNDARRASEAQ"
     gene            3901..>4438
                     /locus_tag="VKQ52_08315"
     CDS             3901..>4438
                     /locus_tag="VKQ52_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLZ87229.1"
                     /translation="MATPLQSNTIALNNARSLNGYASKPMTDIITNGFFIVDRKWVVT
                     HWNKAAENLLSIKAKDILGHNLWEAFAGVLPLEFYAVYHKALLEDIPIHFEEYWEELG
                     GWFDVITYYFADTLSVSFKNSNRSVAKGNPEEQLKTLNELYRYVTEITNDCLWEWDLV
                     GKQLFWIDGGHKRVFGYPI"
BASE COUNT         1054 a         1133 c         1129 g         1122 t
ORIGIN      
        1 aggttttctt aatttgaccg gttttacaaa ctcgggtctg acatgcttta gtacgttcca
       61 caggaactgc cggcgcgcct ttgccggcga atgagcatgc cggcggagaa agagttcggt
      121 aagatcgcct tcaatctctt ccaggtattc ttcacggcag aaccaccgga ggaaaagcaa
      181 tggccatttg ggcggtctcg cattcatcgg ccaaaggata ttttagggat catcttgtaa
      241 aggccggtcc gtaactcata ttgaccgtca agtatgcgtt ttccaagcgg agtgataaca
      301 taatactttt ttcttctgcc gcctctgtct tcggtaatac ctccgaatcg tgatttgaca
      361 aagcctttat cctccatccg tttcagggcg acatggatgg cgctgatatt gatattcttc
      421 gacaatcttt cgagaaggtt ttcctggata ctcaccccgt aggcttcatc atgtaaggca
      481 gccaccagta ggagcaccag ttcctcgaat tctccgatgg aatgatgttt catgttggta
      541 tttacttaac aaaagtaaat ataatgaatt aattccacat ggaggtcaat tcaaaaagaa
      601 gtccaatagg gctttttgaa cctgcgccgt ttgttcctga accacccaat ggccggagtc
      661 cttaataatt acttcatgta cctcattcgc taccagtctg ctgtgatctg ctaaaaacga
      721 gcctgcaaaa tgttcggaac ccatcgccag caagggcatg ggaagtttgt ttttcataaa
      781 tgctgcgtcg tcaatggcat cttgcggaaa ttcgccgaac cagtgaaagg cgctggtagt
      841 ggctcccggc actgaataag cccttataaa ttcatttttc tcggtttgtg taaaggcgct
      901 tttctgatag ccaagcggtg gccagaaatc ggtaaagaaa agacctacat tgcctgatac
      961 gatctcaccg gaatgcggct gggcaaaaaa tccaaaccac caggcctgtg ctttcacctc
     1021 gctatatacc ggttctatac cgggcaacaa agcgtccata aaggctatct ttttcacatc
     1081 gccggggaac tgggcggcgt aggcataggc cacaatcatc ccgatatcat gccctgcgat
     1141 attaatatgc tggtagccca attttgtcat cagttcatgc atatctaccg ccatgttttt
     1201 tttgtcgtaa ccaccggccg gtttgcctga ttcgccaaca ccccggaggt cgggagcgat
     1261 cacggtaaag tgctttgaaa gttcagggag caatcggttc cacatgtacc agttttgccc
     1321 aaaaccatgt actaatagca ggggctcgcc gttgccgcct atcacgtagt ggattttgac
     1381 gctgtttacc acagcgtatt ggtgtttaaa tccggtgggg ggtgaagcgg gcctggggat
     1441 tttcgcagtg ccgttgggag cctgcatggc aggagccttt ccgataaata atacagtggt
     1501 caccgaaagt atcaaggcgg catatagcgt tttgctcatc gttttcatac acatacttgt
     1561 tgatttataa ccaaagtaag gcaggaaaat cgggatgact gtgtgaccta tatcacattt
     1621 aaaaagccac tcctttaggc atagctattg cgcttcggat gccctgcgcg cgtcgttgta
     1681 agctttaacg atagcattga tcacgagggc aggatcatca ttgaatataa aatgcccgca
     1741 acccgttgcc aggatgcccg tccggttgga ggacgctgcg gcgaattccc ggtgacagcg
     1801 cttccagtca ttgatatccg tgctatcctg gaaagggggg tggtcggaaa caaagtccgt
     1861 taccgggatc gtattcggaa aggcgacgtt tctcatggac tcgatattgt cactaaaatc
     1921 cgccccctgg tagtagcggc cgggatccga tgcttttact ttgtccgtat tttgttggtt
     1981 gatcattcgt tgcgttgcgg cgagcctttt ctcatcgtag aagcaggtag tggtgacatc
     2041 gatcagcacg gccgctttca ctttctccgg gtgtcgggcg gcatagagct gtgcgtataa
     2101 tccgccttgg gagtgggcaa ccagcatgat attgttgtcg taacccaatt tttgcaaacc
     2161 catttcgagc ccgaggatgc cattcatgag cccgcgttgg gtcgtatcga aggtgctttg
     2221 gccaaagccc gccctgtcat acgtgatcag ggtggttcct gttcgcttgg caatgggcag
     2281 gaggatctta ttccaggtgg tagcatcctc gccgccgcct gcttcaaaaa gaatagggat
     2341 gcctttgcct ttagaaataa cgaaatgcag tttatatccc ccgacgtcga ccaggatgtc
     2401 gccgtccccg gttttctccc caccggatcc ttgacccgaa gcctgaccgt tgttgcggtc
     2461 aaagccgtcg atcttcgtcg gggtgggaat gtcccatcta ttcggctggg tggcctggat
     2521 attgccatca ggtgagcctt gcccgttcca taaaacatgg cccttgttcc ggccccatac
     2581 ccaaagatgt ccgttattgt cttccgcgac gacataaaag gcatcgccga tgcctgtgaa
     2641 cagcttcacg aaagatacgc cggccggatt gatcttaacc ggcttgtcca cccagagcag
     2701 gccgccgccc cagggcgcga taaaattggc gaattgcgct tcctttccat ttccgatcgc
     2761 ggcctgcgta ttgtcacccc aggcataggc atcgccgttg gacataagag cgtaggatgc
     2821 ctggctgccg gctgccagct gcaccggatt gcccggcagg ccaagcgttt tcgtgagatt
     2881 gaaaggggta ttccgcgcgg agacattggc accgaggccc aggtagcggg ggttgtaggc
     2941 caccccgtaa atggcgccgc ttcttccctt gaagtaggtc cattcccccc cgccgacaag
     3001 atccacgatc ggctcgggga atttcatggc gccgggccgg gtcggatcag gattgtcgac
     3061 tccgcggcca aggacaaggg tcgggtagta tttgcggcgc gtcccgcccc aactgtcgac
     3121 tgtcccgtcc ttgtagagga catagatgaa cgacccgacc gccaccttct ggacgggctt
     3181 tccgatcacc gttacccggt agggccccgg ctcggccttg ttgccctcgg taccatcccc
     3241 cctgaatccc gattgggtgt tccccaataa cgtcagctta ttgtcgttgg tgacgatagc
     3301 gaaaccatat cccaggcctt gccccgaacc atcaccggag ttggcatagg caacgacctg
     3361 ccgggcattc gagaccgccg ttttcgtcat cccgatccac ggtagcttaa ggccgagacc
     3421 ggagatgtta caggcattgt ccccccagac atacacgtat ccatcgacac cgataaaacc
     3481 cgccgtatga agcgacgcgg agcccttgcc atcgccatcg gcaatggcag gaaggccggc
     3541 caccgattcg gtgctgctgg aaacaatatc gaacagcagc ttggtcttgg gatggcgtac
     3601 ccagttatcg tactccccgg gatagatttg gtacttctgc gcatacgaca caagcgggaa
     3661 gagaagaagc caaagaatat ttttcatata tcaacactaa gatacgggaa cctgagcata
     3721 tccctccaat cctggcgggt cttgaccagg tggttctcgg cccggttcag gaaggtcacc
     3781 ggcgacgata tgtgaattgt ataacttatc cttacattta tgtaacaact accatcgtaa
     3841 aacaagccac ctttgcaagt gattgctttc catagcgatc gatcaggtaa tgggtaaaaa
     3901 atggcaactc cacttcaatc aaatacaata gcacttaata atgctcgcag cctcaacgga
     3961 tatgcaagta aaccgatgac agacataatt accaatgggt tttttatcgt ggatcgtaaa
     4021 tgggtagtga cgcattggaa caaagcagca gaaaatttgc tgtccattaa ggcaaaagat
     4081 attctggggc ataacctatg ggaggcattt gccggagtcc ttccattgga attctatgct
     4141 gtttaccaca aggccctcct ggaagatatt cctattcatt ttgaggagta ttgggaggaa
     4201 ttgggaggct ggttcgacgt aattacctat tatttcgcag acaccttgtc cgtttcattt
     4261 aagaacagta atcgttcggt cgccaaggga aatcctgaag aacagcttaa gacactaaac
     4321 gaactatacc ggtatgtgac ggagattaca aacgattgtc tttgggaatg ggaccttgtc
     4381 ggaaagcaat tgttttggat agatggagga cacaaaagag tttttggcta tcccatcg
//
LOCUS       DATGFG010000449         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_68771, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000449 DATGFG010000000
VERSION     DATGFG010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68771"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VKQ52_08320"
     CDS             <1..799
                     /locus_tag="VKQ52_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ87230.1"
                     /translation="ALVLLIFYLYKKNVLRFHIRSREAEASRDAGENLNNLKNSFFQN
                     ISHDIRGAYFGVGSICAVIHDKVATDEPVTPQLAENLMVASQNYKYMLNHFLEFSRFK
                     MGTVDEIVPETIDVAAEVAQIVQLNRFLAEEKNIRVDIHRDDHFPSTLLTDRLKITRI
                     FYNIFVNALKFSSAGSRVRIHLGMGDAVWILRVIDQGKGFHPDHLVRIFEPYATNRSP
                     ENPEGIGLGLYITNYLAKLLGGEIDIISNPGAGATVELRFPLHDNRA"
     gene            complement(779..1897)
                     /locus_tag="VKQ52_08325"
     CDS             complement(779..1897)
                     /locus_tag="VKQ52_08325"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87231.1"
                     /translation="MQPEQNVRAVSSAAMNRIQHEYARLISQNFCVSLLDLFDSGTFH
                     LERYCEDYHPHPQMQEIIRNIIGYGHSMGILLPNAEHYLTCALYLFPDASPEKILLIA
                     RNYAVDFYLNDTMGREYRSTSEERQRLNEIRARLAGRADLGNVDETASAAERANLDVL
                     AEIARTSPESWFESFLSLYLRHLSVAHRSYDCMSLGYIPKIEEYIAIRDDISGMPHTV
                     SMIEYSQGYYLDWPALEAVGLAEDLRDLGTTVSLIGALMNDVFSFEKEVIDNRSDSNL
                     LAIIVLNNFKMELREAMSVATTIVRNLLIEYEQHVRVIAEKIAALPDVEERERLGRYM
                     DGIKKCVQTCWLWQVDTRRYKRGRSIWRETSLSGPVVV"
     gene            2034..>2221
                     /locus_tag="VKQ52_08330"
     CDS             2034..>2221
                     /locus_tag="VKQ52_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87232.1"
                     /translation="MSHKPLIIFLLALTALSCHNNSGPSMPATTPPPAKPGPAPNPLE
                     SDTLIARNTGIDSAHACS"
BASE COUNT          522 a          711 c          514 g          474 t
ORIGIN      
        1 cgccctcgtg ctgctcatct tctacctcta taaaaagaac gtcctccggt tccatatccg
       61 gtcgcgcgaa gccgaagcct cccgcgatgc cggcgagaac ctcaacaatc tcaagaatag
      121 cttctttcag aatatctccc atgacatcag gggcgcttac tttggcgtag gaagcatctg
      181 tgccgtgatc cacgacaagg tcgccaccga tgagcccgtg acgccccagt tggccgaaaa
      241 cctgatggtg gcttcgcaga actataagta catgctcaac cacttcctgg aattctcgcg
      301 gttcaaaatg ggaacagtgg acgagatcgt tcccgaaacc atcgacgtag cggccgaagt
      361 agcacagatc gtacaactca accgcttcct cgccgaagag aagaatatcc gggtggacat
      421 tcaccgcgat gatcacttcc cttccacact ccttaccgac cgcctgaaga tcacccgtat
      481 cttctacaat atctttgtca acgccctgaa attctcttct gccggttccc gcgtccgtat
      541 tcacctgggg atgggagacg ccgtctggat cctgcgcgtc atcgatcagg gcaaaggttt
      601 ccaccccgac cacctggtgc gcatcttcga accctacgcg acaaaccggt cgccagaaaa
      661 tcccgaaggc atcggccttg ggctgtacat caccaactac ctcgcaaagc tcctcggcgg
      721 ggagatcgac atcatctcca atcctggcgc cggcgccacc gtcgaattgc gtttcccctt
      781 acacgacaac cgggcctgac agggaggttt ccctccagat cgaccgcccg cgcttgtacc
      841 tcctcgtatc cacctgccaa agccagcagg tctgcacaca ctttttgatc ccatccatat
      901 atctgcccag ccgttccctt tcctccacat cgggcaaagc ggcgatcttt tcggcgatga
      961 cccgtacatg ctgttcatac tcgatcagca ggttacgaac gatcgtcgta gccaccgaca
     1021 tcgcctcgcg cagctccatc ttgaaattgt tgagcacgat gatggccagc aggttcgaat
     1081 cgctccggtt gtcgatcacc tccttctcaa aggaaaaaac atcgttcatc aacgcaccga
     1141 tcagggaaac ggtcgtgccc agatcacgca gatcctccgc cagacccacc gcttccagcg
     1201 ccggccagtc caggtaataa ccctggctgt attcgatcat cgaaacggta tgcggcatcc
     1261 ccgagatgtc gtcccggata gcgatatact cctctatctt ggggatataa ccaagtgaca
     1321 tacaatcata gctccggtgc gccaccgaaa gatgccgcag atagagcgac aggaatgact
     1381 cgaaccagga ctccggtgag gtccgcgcaa tctccgccag cacatccagg ttggcccgct
     1441 ccgccgcgga cgcggtctcg tcgacattcc ccaggtctgc ccttccggca agccgtgccc
     1501 ggatctcgtt cagccgttgc cgttcctccg aggtcgaccg gtattccctc cccatcgtgt
     1561 cattcagata gaaatccacg gcataattgc gggcgataag caggatcttc tccggcgaag
     1621 catcggggaa caggtacaac gcacaggtga gataatgctc cgcattgggc agcaggatgc
     1681 ccatggaatg gccatagccg atgatattcc ggatgatctc ctgcatctgc ggatgtggat
     1741 gataatcctc gcaataacgc tccaggtgga atgtgccgga atcaaaaaga tcgagaaggg
     1801 aaacacaaaa attctggctg atcaggcggg cgtactcatg ttgaatacgg ttcatggcag
     1861 cggacgacac tgcccgaaca ttttgttcag gttgcataat aggtaaagtt taaatggtta
     1921 ctttctttaa gttacaaaaa aaagccgctc gttggagcgg ctttgttgag tttctcaaaa
     1981 ccccttgcca ctactccctc cggaaatggc aacgtatttg tattgatgaa tttatgtccc
     2041 acaaaccatt gatcatattc ctcctcgctc tcaccgccct ctcctgtcac aacaactccg
     2101 gcccctccat gcccgctaca accccaccac ccgccaaacc cggccccgcc cccaatccac
     2161 tggaaagcga taccctcatc gcacgcaaca ccggcatcga ctccgcacat gcctgtagcg
     2221 a
//
LOCUS       DATGFG010000450         7676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_641743, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000450 DATGFG010000000
VERSION     DATGFG010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641743"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2160)
                     /locus_tag="VKQ52_08335"
     CDS             complement(<1..2160)
                     /locus_tag="VKQ52_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLZ87233.1"
                     /translation="MPLESHFFEDVLSLVPAEIIVADAEYRFLYANASAIPDQHLREW
                     MIGRTNEEFCQYAGRPAAMARARRMVFEKARRTRRVVEWAESVMDSEGVVQHFQHRVF
                     PVFDEAGNLHQVIIYAVRITETKEFEEKVKVSEKRYRDLFNHSQALICTHDLDGKLLS
                     VNPAIRKVLDFEESEMLGRSIQDFVPDKHRKRFEEEYLHAIRQSVNAVSGEFCVLNKS
                     GEEIYLLYKNVRMEEPGSEPYVIGFSQDITDRIHMERELRITKQMTDDIARAKESFLA
                     HMSHEIRTPMNGILGIASLLNKTRLDPQQRNYLQLIQESANNLLVIVNDILDLEKIVA
                     GKLQLERIPFKIVDKIATTIQSFIYRAEEKELGLVFQNSIPADLVVDGDPYRLSQVLN
                     NILGNALKFTNEGHINITTGIREHDEEEVVVEIIIQDTGIGIGKERLQTIFEPFEQAD
                     ATISRKYGGTGLGLTICKNMVEMQNGELLVQSEEGKGSAFTIRVPYLISIEAMQENEV
                     SQEIDYKSLGPRKVLVAEDVELNQYLARHILESWDFEVVIVNNGREALEALEKSSFDC
                     ILMDVQMPEMDGIEATQHVRKMPDPVKAGIPIIALTANALKGDSEKYLAAGMTDYLAK
                     PFDEARLFRVISRNLTRLQDPVGSATPIATAHSHNTNNSNMIPGNARLYDLSMVQSVS
                     GGDEGFIKKMVALFIETVPQNMRDLKNALKEENWDQVG"
     gene            complement(2189..3550)
                     /locus_tag="VKQ52_08340"
     CDS             complement(2189..3550)
                     /locus_tag="VKQ52_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLZ87234.1"
                     /translation="MKNHHSFKVFIVEDDVWYGSMLQHYLSLNPEYEVKRFESPRDFF
                     AQLHENPDVVTLDYSLPDCDGAEVLKKIKEHNPDIRVIVISGQEDVATAINLLKNGAF
                     DYIVKDDDTKDRLWNSILHLREISNLKQEVETLKSQVGRKYDFSQMIIGKSEQIQKVF
                     SMIEKASKTNITVSVTGETGSGKEMIAKAIHYNSERHKLPFVAVNVAAIPKDLIESEL
                     FGHEKGAFTGAVTRRIGKFEEADKGTLFLDEIGELDINLQAKLLRVLQEKEITRIGSN
                     AVTKIDARIVVATHKNLLEEVKNKNFREDLYYRLIGLPITLPPLRERGNDILILAKHF
                     IDGFCKENKMDKRTLSPEAQTKLLTYPFPGNVRELKSVVELAVVMADEETILGEHITL
                     NTSASIADLLNKEMTLKEFEIQILQHYLDKYDKDVLLVAKKLDVGKSTLYRMIQNGEL
                     KMK"
     gene            3760..6921
                     /locus_tag="VKQ52_08345"
     CDS             3760..6921
                     /locus_tag="VKQ52_08345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HLZ87235.1"
                     /translation="MSLNREKLRQHFEEEYQKLNEQQRLAVDTTEGPVMVIAGPGTGK
                     TQILAARIGKILLETDAAPGNILCLTYTDAGTIAMRRRLLSFIGPDAYKVHIHTFHSF
                     CNDIIQENLPLFEKTALDAISDLERIQLFKELIDGFPKNHPLKRYRGDVYYEMGNLQS
                     LFSTMKREGWTPAFIDGKVDDYLADLPNREEYIAKRATKEFRKGDLRTDKIEEEKERM
                     NKLRAAVSEFDRFQELMRRHNRYDFDDMINWVIRAFEGNRALLSSYQERYQYILVDEY
                     QDTSGTQNRLVSLLITYWEQPNVFVVGDDDQSIYRFQGANVENMLDFAHQYQKDLLTV
                     VLTNNYRSTQPILDISRALIERNEERLVRQLPGLSKQLVASHPRTGKLHYPAQLLEYL
                     SPRQEMTGIALQVEKLLAEGVRPGHIAIIYKENKYGEELARFFQLKKIPVYSKRNLNL
                     FTVPLAQQVLLLLRYLAAEHDAPYGGDEMLFEILHFHFWSIAPIEIAKLSVEVADRQF
                     GEQRTSIRRLLCEKAARPARDLFDTGLPEGLKKASSVLEKLIGDVSNSTLQSLFENCI
                     RDTGILNAVLTGADKIGQMQVLTALFDFIKEETRRDPDLRLEQLIQMIDLMKDNEIPL
                     PLVRVSGNDKAVNLLTAHGSKGLEFEYVFFAGANASTWEKKRRPGGGYKYPDTLFASA
                     AGAVSSGDEELRRLFYVALTRVEQRLTISYSRYRDDGKELEPSVFLAEILESQPLAFE
                     KVTIPAEEQAAFDALPFSDAIAPEIQAAEEDFIGRMLEKFVMNVTALNNYLKCPLEFY
                     YRNLVRIPSPKNEATEFGSAVHFALQRLFEKMQLHAGEQFPAKEEMLADFDWYMRRHR
                     EIFTREAFARRMEYGLEVLANYYDKYLYQWNRIVAVERNIRNVVVRGVPLKGKLDKLE
                     FQGKEVNVVDYKTGDVDRATDKLKPPGAKDPNGGDYWRQAVFYKILVDGYEQKDWKVT
                     STEFDFVEPDKKKEYRKVKIVITPEDITTVTQQIIQTWQKIQNREFYTGCGKPDCHWC
                     NFVKTNNLAIALHEIGEEEEEEV"
     gene            6971..>7676
                     /locus_tag="VKQ52_08350"
     CDS             6971..>7676
                     /locus_tag="VKQ52_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HLZ87236.1"
                     /translation="MKIKTGLFFLILFSLLIVCIVGPTGCANIVAPLGGPKDTIPPRL
                     VSAVPKDSAKHFTGTKIIFNFDEYIDGKDIRNELLVSPVPKIDPIIDTKLRTVTVRIK
                     DTLQPGTTYALFFGKGIKDVNEGNVLRNFTYVFSTGDTIDRGDFTGSVFVANTGKPDS
                     TLVAVLHDKFDDSAVVKNRPRYIAKCDSTGRFHFRFVKPGVYALYAMKDEGGSHKYLS
                     KAQLFAFADSPVTVGTT"
BASE COUNT         1680 a         2013 c         2109 g         1874 t
ORIGIN      
        1 acctacctgg tcccaattct cttccttcag ggcattcttc agatcccgca tgttctgcgg
       61 caccgtttcg ataaaaagcg cgaccatttt ctttataaaa ccttcatcac caccggacac
      121 ggattgcacc atggagaggt catataatct ggcattccca ggtatcatat tgctattgtt
      181 agtgttatgc gaatgggcgg tagcaatcgg agtggcagaa ccaacggggt cctggagacg
      241 ggtcaaattt cgggaaataa cgcggaaaag ccttgcctca tcaaagggct tggcgagata
      301 atcggtcatt cccgcggcaa gatatttctc gctatctcct ttaagggcat ttgcggtgag
      361 ggcgatgatg gggatgcctg cctttaccgg gtcgggcatc ttccggacgt gctgggtggc
      421 ttcgatgcca tccatctccg gcatctgtac atccatcagg atacagtcga aggagctttt
      481 ttccagcgcc tcgagggctt cgcggccatt gttcacgatc acgacctcga aatcccatga
      541 ttcgaggata tgccgggcga gatattgatt caactccaca tcttccgcca ccagtacctt
      601 ccggggtcca aggcttttgt agtctatttc ctggcttact tcattttcct gcatagcttc
      661 aatacttata agatagggga cgcgtattgt aaaggccgat cccttgcctt cttcactttg
      721 taccagcaat tcgccgttct gcatttcgac catattctta cagatagtca atcccagccc
      781 tgtcccccca tacttccggg agatggttgc gtccgcttgt tcaaagggtt cgaatatagt
      841 ttgtaaccgt tctttcccaa ttcctattcc ggtgtcctgg ataatgatct ccaccaccac
      901 ttcctcttcg tcgtgttccc ggataccggt ggtgatattt atatgtccct cgttggtaaa
      961 tttcagtgcg ttgcccagaa tattgttgag cacctggctg agccggtagg ggtcgccgtc
     1021 caccacaaga tcggccggga tcgaattctg gaagacgagc cccagctcct tctcctccgc
     1081 gcgatagatg aacgactgga tcgtcgtggc gatcttgtcg acgatcttaa agggaatccg
     1141 ctcgagttgc aatttccctg ccacgatctt ctccagatcg agaatgtcgt ttacgatcac
     1201 taataagttg tttgccgact cctggatcag ttgcaggtaa ttgcgttgct gcgggtcgag
     1261 gcgggtcttg ttcagcaggc tggcgatccc gaggatgccg ttcatcgggg tgcggatctc
     1321 atggctcatg tgggccaaaa aagattcttt tgcccgggcg atgtcgtcgg tcatctgctt
     1381 ggtgatgcgc agttcccgtt ccatgtggat cctgtccgtg atatcctggg agaagccgat
     1441 gacgtagggc tcggagcccg gctcttccat gcggacgttc ttatagagga gatagatctc
     1501 ttcgccgctt ttgttcagca cgcagaattc cccgctcacc gcgttgacgg actgccggat
     1561 cgcgtgcagg tattcttcct cgaaccgttt gcggtgttta tccgggacga aatcctggat
     1621 gctgcggccc agcatttccg actcttcgaa gtcgaggacc ttgcgaatgg cgggattcac
     1681 ggaaagcagt tttccgtcga ggtcgtgggt gcagatgagg gcctggctat ggttgaacag
     1741 atcgcggtat cttttttcgc tgacctttac cttttcctcg aattcctttg tctcggtgat
     1801 gcgtaccgcg tagatgatga cctggtgtaa atttcccgct tcgtcgaaga cggggaatac
     1861 tctatgctgg aagtgttgta cgacgccctc gctgtccatg accgactctg cccattccac
     1921 caccctgcgg gtgcgccgtg ctttttcgaa taccatgcgc cttgcccttg ccatcgcggc
     1981 cgggcggccg gcatattggc aaaactcctc attggtgcgc ccgatcatcc attcacggag
     2041 atgctgatcc ggtatagcgc tggcattagc gtacaggaac cggtattcgg catccgccac
     2101 gatgatctcg gcgggaacaa ggctaaggac gtcctcaaaa aagtggctct ctaaaggcat
     2161 gcacgggccc gtttataggc attttggttt atttcatctt caactccccg ttctggatca
     2221 tgcgataaag cgtggattta cctacatcga gcttttttgc cacgagcaga acatccttgt
     2281 catacttgtc cagataatgc tgcaggatct ggatctcaaa ttccttaagg gtcatttctt
     2341 tattcagcag atcggcgatg ctggccgagg tgttgagcgt gatgtgttcg cccaggatcg
     2401 tctcttcgtc cgccatgacg acggccagct ccaccactga tttcaattcg cggacgttac
     2461 ccgggaaggg gtaggtcagc aactttgtct gggcctccgg tgacagggtc cttttgtcca
     2521 ttttgttctc cttacagaac ccatctataa aatgcttggc gaggatgaga atgtcattcc
     2581 cgcgttcgcg caggggtggc agggtgatgg gaagaccgat caaccggtag tacaagtctt
     2641 cccggaagtt cttattcttt acctcttcca gcagattttt atgggtggcc accacgatcc
     2701 gggcatcgat ctttgtgacg gcattactcc cgatacgggt gatctctttt tcctgtaata
     2761 cacggagcag ctttgcctgc aggttgatat ccagctcccc gatctcgtcc aggaagaggg
     2821 ttcccttgtc ggcctcttcg aacttcccga tgcgccgggt caccgcgccg gtgaacgccc
     2881 ccttctcatg accaaagagt tcgctttcga tgagatcctt ggggatcgcg gccacgttga
     2941 cggccacgaa gggcagttta tgccgttcgg agttataatg gatcgccttg gcaatcattt
     3001 ctttaccaga tcccgtctca ccggtgacag acacggtgat gttcgttttc gaggccttct
     3061 cgatcatgga gaagaccttt tggatctgtt cgctcttccc gatgatcatc tgggagaagt
     3121 cgtatttccg gcctacctgg cttttcaggg tttccacctc ctgtttcaga ttgctgatct
     3181 cgcggagatg caggatgctg ttccagaggc ggtcttttgt gtcatcgtcc tttactatat
     3241 agtcgaaggc cccgttcttg agcagattga tggccgtggc cacatcctcc tgaccggaaa
     3301 tgacgatgac ccggatatcg ggattgtgct cttttatctt ctttagcact tcggccccat
     3361 cgcagtcggg cagagaataa tccagcgtca caacatccgg attttcatgt agttgggcaa
     3421 aaaaatcgcg gggggattca aatcgcttta cctcgtactc cggattaaga gagagataat
     3481 gctggagcat cgagccatac cagacatcat cttcgacgat aaagacttta aaagaatgat
     3541 ggtttttcat gcgcgtttac ttaggatagg aatctaacgt tgaaatggct aatttattcc
     3601 catattaaga cattttccaa tccattctcg ctcttttcga cgaattaaga ctttgggggt
     3661 tgatattaag tggaaactct taacctccgg gggctgattt ttgcacgccg gttgatttct
     3721 taagaacaac ccgctggtgc aaaatcttac ctttggacca tgtcgctaaa ccgggaaaaa
     3781 cttcgccagc attttgagga ggagtaccag aaattaaatg agcagcagcg tcttgctgtg
     3841 gataccacgg aaggccccgt gatggtcatt gccgggccgg ggacgggcaa gacccagatc
     3901 ctggcggccc ggatcgggaa gatcctgctg gaaacggatg cggctccggg taatatcctt
     3961 tgtcttacct atacggatgc cgggaccatc gccatgcggc ggcggttatt gtcctttatc
     4021 ggtcccgatg cctataaggt ccatatccac acttttcatt ccttttgcaa cgatatcatc
     4081 caggagaacc tgcccctgtt cgagaagacg gcgctggatg ccatttccga cctggaacgc
     4141 atccagttgt tcaaggagct gatcgatggt tttcccaaga accaccccct gaagcgctat
     4201 cgcggggatg tctattatga aatgggcaac ctgcagagcc tgttctccac gatgaagcgg
     4261 gagggctgga ctccggcctt tatcgacggg aaggtcgacg actatcttgc cgacctgccc
     4321 aaccgggaag aatatatcgc caagagggcg acaaaggagt tcaggaaggg ggatctccgg
     4381 accgacaaga tcgaggagga aaaggaacgg atgaacaagc taagggcggc cgtgagcgaa
     4441 ttcgatcgct tccaggagct gatgcggcgt cataaccgct atgatttcga tgacatgatc
     4501 aattgggtga tcagggcctt cgagggtaac agggccctgc tttcgtccta ccaggagcgt
     4561 taccagtata tccttgtcga tgaataccag gacaccagcg ggacacaaaa ccggcttgtg
     4621 agtctcctga tcacgtattg ggagcagccg aatgtctttg ttgtgggcga tgacgatcag
     4681 agcatttacc gtttccaggg ggccaatgtg gagaacatgc tggactttgc ccaccagtac
     4741 cagaaggacc tgctcaccgt cgtgctgacc aacaattacc ggagcaccca gccgatactg
     4801 gacatttcga gagcgttgat cgagcggaac gaggagcggc ttgtccggca gctgcccggg
     4861 ctttcgaagc agctggtcgc gagccatccc cggacgggca agctgcatta cccggcgcaa
     4921 ctgctggaat acctgagccc ccgccaggag atgacgggga tcgcgctaca ggtggaaaag
     4981 ctgctggccg agggggttag gcccgggcat atcgccatta tctataagga gaacaaatat
     5041 ggggaggagc tggcgcgctt cttccagctg aagaagatcc cggtctacag caaacgcaat
     5101 ctcaacctgt ttaccgttcc gctggcgcag caggtcctcc tgctcctgcg ctacctggcg
     5161 gctgaacatg acgcgcccta tggaggggat gagatgcttt tcgagatcct tcactttcat
     5221 ttctggtcca ttgcgccgat cgagatagcg aagctttccg tcgaggtcgc ggaccggcaa
     5281 ttcggggagc agcggacctc tatccggcgg ttgctctgtg agaaggcggc acggccggcg
     5341 cgtgatctgt ttgatacggg gctgcccgaa ggcctcaaaa aggcgagttc cgtgctggag
     5401 aagctgatcg gggatgtatc caacagcacc cttcagtcgc tgttcgagaa ctgtatccgc
     5461 gacaccggca tcctgaatgc ggtgttgacc ggtgcggaca agatcgggca gatgcaggtg
     5521 ctgaccgcgt tgttcgattt tatcaaggag gagacccggc gggaccccga tctccgcctg
     5581 gagcagctga tccagatgat cgatctgatg aaggacaacg agatccccct accccttgta
     5641 cgggtgtcgg ggaatgacaa ggccgtcaac ctgctgaccg cgcatggctc caaaggcctg
     5701 gaattcgaat atgtcttttt tgccggcgcc aatgcttcca cctgggaaaa gaagcggcgg
     5761 ccgggcggcg gttataaata cccggacacc ctgttcgcct ccgcggcggg tgcggtatcc
     5821 agcggagatg aagaattgcg gcggcttttt tatgtggccc tgacccgggt ggagcaacgc
     5881 ctgaccattt cctattcccg ttaccgggat gacggaaagg agctggagcc ttcggtcttt
     5941 cttgccgaga tcctggagag ccaacccctg gcttttgaaa aggtgacgat cccggccgag
     6001 gagcaggcag cctttgacgc cctgccgttt agcgatgcca tcgcccccga gatccaggcg
     6061 gcagaggagg attttatcgg ccggatgctg gaaaaattcg tcatgaatgt gacggcgctg
     6121 aacaattacc tgaagtgtcc gctggagttc tattaccgga acctcgtccg catcccatcg
     6181 cccaagaacg aagccacgga attcgggagc gcggtgcatt tcgccttgca gcggctcttc
     6241 gagaaaatgc aattacatgc cggggagcag ttccctgcca aggaggagat gctggcggat
     6301 ttcgattggt atatgcggcg ccaccgggag atctttacga gggaagcgtt tgcccgccgg
     6361 atggaatatg gcctcgaggt gctggccaac tactatgata aatatctcta ccagtggaac
     6421 aggatcgttg cggtagaacg gaatatccgc aatgtcgttg tccgcggtgt accgttgaag
     6481 ggcaaactgg ataagctcga attccagggg aaagaggtga atgtcgtcga ttacaagacg
     6541 ggtgatgtgg acagggccac cgataaactg aaaccacccg gcgcaaaaga ccctaacggc
     6601 ggggactact ggcggcaggc tgttttctac aagatactgg tcgacgggta tgagcagaag
     6661 gattggaagg tgaccagtac ggagttcgat tttgtggagc ccgacaagaa aaaggaatac
     6721 cggaaggtga agatcgtcat tacgccggaa gacattacca ccgtcacgca acagataatc
     6781 cagacctggc agaagatcca gaaccgcgag ttttatacgg gttgtggcaa acccgactgt
     6841 cattggtgta atttcgtaaa aaccaataat ctggcgattg ccttacacga gatcggcgag
     6901 gaggaggaag aggaggtgta ggcgttggaa ataacatttt gttgcggttt aaacctatta
     6961 tgtttgcaga atgaagatca agaccgggct gttttttctt atattgttct ccctgctcat
     7021 cgtttgtatc gtcggaccca cgggatgtgc gaatatcgtc gcgccgctgg ggggtccaaa
     7081 ggatacgatc ccgccccggc tggtctcggc agtcccgaaa gattccgcca agcatttcac
     7141 cgggacaaag atcatcttca attttgacga atacatcgat ggaaaggata tccggaacga
     7201 gctgcttgtc tcgcccgtgc ccaagatcga tcccatcatc gatacgaaat tgcggacggt
     7261 cacggtcagg atcaaggata cactgcaacc cggcactacc tatgccttat tttttggaaa
     7321 gggcatcaag gatgtgaacg aaggcaatgt cctccgcaac ttcacctatg tcttttcgac
     7381 gggcgatacg atcgaccggg gcgatttcac cggaagcgta ttcgtcgcca atacgggtaa
     7441 gcccgacagc acccttgtcg cggtgctgca cgataaattc gatgattccg ccgttgtcaa
     7501 gaaccggcca cggtatatcg ccaaatgcga cagcacgggc agattccatt tccggtttgt
     7561 caaacccggg gtctatgcgc tttatgcgat gaaagacgag ggcggctcgc acaaatatct
     7621 ctccaaggcc cagctgttcg cctttgccga ctcccccgtc accgtcggca cgacca
//
LOCUS       DATGFG010000451         4651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_708972, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000451 DATGFG010000000
VERSION     DATGFG010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708972"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VKQ52_08355"
     CDS             complement(<1..287)
                     /locus_tag="VKQ52_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87237.1"
                     /translation="MRYVLFILILLFTLSGLRAQNVYTITADSVKLTGCDSSELIIEN
                     HTRAVPGFLFNTGNGRTIFKRVMQKLSDSQYLLGPDTLQIHFPNAWLQGGNA"
     gene            complement(311..1261)
                     /locus_tag="VKQ52_08360"
     CDS             complement(311..1261)
                     /locus_tag="VKQ52_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87238.1"
                     /translation="MGLRAQSVYTITADSVKLTGCDSSELIIENHTRATPGFLFNTGN
                     GRTVFKRGLQQLSASSYLIGADTLQLAVNPWVQGGNSFGADGVLGLKDNHLLDIIQGS
                     TSRMRFFSNGNVGIAQTVDNGYRLNVNGSIYSAGFLFGAEGAALTGPLSFDASAAQNI
                     IYGNIITFNTNNSQGNTVDGDYIFTNQPPQANSGGGHSRLIHVNKQVNRGTQDGEETA
                     LRIDFSVTGTANDLRAIHAATGNVVVETGNLLIGAATGSTSKLDITGANGYSQLRIRT
                     QYTPSSSADTNGNPGDIAVDDNYFYYKSSSGWKRVAMSSF"
     gene            complement(1312..2907)
                     /locus_tag="VKQ52_08365"
     CDS             complement(1312..2907)
                     /locus_tag="VKQ52_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87239.1"
                     /translation="MKAQNVYTIKADSVKLTGCDSSELIIENHTQNIPGFLFNTGNGR
                     TVFRRGAQKLNDSLYLVGSDTLNLGAKSWVQGGNAFGATGVLGTLDYYPLDLYTNGTP
                     RARLTPGGNFLLGTTTDNGNLLQVNGPASFSGNVNIGNATITNGGQLINTNAILFNHV
                     VSQYHAYQQNDILISRLENVLYNYQGRFMTTTSTGSDGTLTIDIVIPPGELAYWAPGI
                     VYPQGKVCLSFWEGGIPQSVSVQMFDNNRKAWAGPFTSTTNLSPQTMGYFEVGIPGSF
                     NYVNEIKIAMTPPAGGGINLQVLEYVLSGDNEGLANPNPYVSKSGDERLYNSLYFYNG
                     GINNVCISSWLPNYFLNNVLVGSATDNGSGNKLQVTGGMSATGGVQFSGLGGDNTQTR
                     VLVADASGNLYYRDASTLAANDLIRGSLAVNGTITAKKLTLTAKDWPDYVFDRGYRLP
                     ALSGVERYIRQNHHLPGITAAAEAEKQGVDVGDNQAALLRKVEELTLYVIDQNKKLEA
                     QNKKIETLSREITKLKRPERIKH"
     gene            complement(2959..4374)
                     /locus_tag="VKQ52_08370"
     CDS             complement(2959..4374)
                     /locus_tag="VKQ52_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87240.1"
                     /translation="MNIRIGILLIFLLFGWSFRTEAQSVYTIQADSVKLTGCDSSELI
                     IGNHTQNIPGFLFNTGNGRTIFKRGAQLLGNGYYLIGADTLKLNPNAWVQGGNSFGST
                     GVLGTLDNNHLDLYTNNVARARLSNTGHFLIGTTADDTASYLLRVKGGGIMMDGGPFY
                     KKYYTDRLQASLKIGSGYELYGVDHASGGYVLYISGGLDGSFDAGWQLAFGNNNNQIM
                     VLSDSLITTNRRLLIAGGLDMLEQYSYRSSPYVGFQYESHDGWDWAVVNSDGNPNLVV
                     TYAGNTLVGATADNGNKLQVGGNSYLNGNSILTGNVRLSGLTSDTTQTRVLVSDAGGN
                     LYYRDASTLAAGEVLRSSLAVNGPITAKKLRLTAKDWPDYVFNSGYRLPPLAGVESYI
                     RQQHHLPGFPSAGEVEKNGVDVGSTQAALVKKIEELILYNIAQEKRLTEQDKRMGAQE
                     KEIGLLKEEIDALKRLVIKQP"
     gene            complement(4376..>4651)
                     /locus_tag="VKQ52_08375"
     CDS             complement(4376..>4651)
                     /locus_tag="VKQ52_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87241.1"
                     /translation="DSRYRLPGLAGVESYIRQQHHLPGLPSAAEVEKYGADVGGTQVA
                     LLKKIEELTLYTIDQDKRLESQHMEIDALKKEVEALKELIKTQIRNK"
BASE COUNT         1009 a         1312 c         1193 g         1137 t
ORIGIN      
        1 gcattcccgc cctgcaacca cgcattgggg aaatggatct gaagcgtatc cggccccaac
       61 aggtattgac tgtcactcag cttttgcatc acccgcttga agatcgtccg tccattgccg
      121 gtattaaaga ggaaccccgg aacagcccgc gtatggttct cgatgatcag ctccgacgaa
      181 tcacaacccg tcagcttgac gctgtccgcc gtgatggtat agacattctg agcccgtagc
      241 cccgaaaggg tgaacaaaag gatcagtatg aataatacat atctcatgag gccgttttta
      301 tctttcactt ttaaaatgaa ctcattgcca cccgcttcca tcccgacgag cttttataat
      361 agaaataatt atcgtctacc gcgatgtcac cgggattgcc attcgtgtcc gccgaagaac
      421 tgggcgtata ctgggtccgg atccggagct ggctatagcc gttagccccg gttatgtcca
      481 gtttggacgt gctcccggta gccgcgccga tcaacaggtt acccgtctcc acgaccacat
      541 tgccggtggc cgcgtgaatg gcccgcaggt cgtttgctgt tcctgtaacc gaaaaatcga
      601 tgcgaagggc ggtctcctcg ccgtcttgcg tgccccggtt cacctgcttg ttgacatgga
      661 tcagccggga gtgaccgcca cctgaattgg cctgcggagg ctgattggta aaaatataat
      721 cgccgtctac cgtattaccc tgggaattgt tggtgttaaa ggttatgata ttgccgtaga
      781 tgatattttg ggcggccgac gcgtcaaaac tcaagggccc cgtcagggct gcgccctcgg
      841 cgccgaacag gaacccggca gaatagatcg aaccattgac attcagtctg tagccattat
      901 ccaccgtctg agcgataccg acatttccat tgctgaaaaa acgcatccgg gaagtgctgc
      961 cctgtatgat gtcgagaagg tggttatcct tcagacccag gactccatcc gccccaaaac
     1021 tgttaccccc ctgtacccag gggttgacgg cgagttgcag ggtgtcggcc ccgatgagat
     1081 agctgctggc gctcaactgt tgcagccccc gcttgaaaac cgtacggccg ttgccggtgt
     1141 tgaacagaaa gcccggggtg gcccgcgtat ggttctcgat gatcagctcc gacgaatcac
     1201 aacccgtcag tttgacgcta tccgccgtga tggtatagac ggactgggcc ctcaggccca
     1261 tcgaaaggcc aagcagaatt accaataaaa gaggacaagc gggtttcatg atcaatgctt
     1321 tattctttcc gggcgcttta acttcgtgat ctcccgggat aaggtttcga tctttttatt
     1381 ctgtgcttcc agtttcttat tctgatcgat gacatacagc gtcagttctt ccaccttcct
     1441 tagcagcgcc gcctggttat cgcccacatc gacaccctgc ttttccgctt cggccgccgc
     1501 ggtgatcccg ggtaaatgat ggttttgccg gatgtaccgc tccacaccgg aaagggcagg
     1561 cagcctgtag ccacggtcga acacataatc cggccagtcc tttgccgtca gcgtcagctt
     1621 tttcgcggta atggtcccgt tgacagccag ggagcccctg atgagatcat tggcggccag
     1681 ggtcgaggcg tcacggtagt aaagattgcc gctggcatcg gctaccagca cccgggtctg
     1741 ggtattgtca ccgccaagcc cggagaactg tacgccaccg gtggcggaca tcccgcctgt
     1801 gacctggagc ttgttgccgc tgccgttgtc ggttgccgac cctaccagta cattattcag
     1861 gaagtagttg gggagccagg aggagataca aacattatta attcccccat tatagaaata
     1921 caaggaattg tagaggcgtt cgtcacccga tttcgagaca taggggttgg gattggcgag
     1981 accttcattg tcgccgctga gcacgtattc gagcacctgc aggttgatcc ctccaccggc
     2041 cggcggggtc atggcaatct ttatctcgtt gacgtaattg aacgatccgg gaatgcctac
     2101 ttcgaaatac cccatcgtct gcgggctcag attggtggtc gaagtaaagg gccccgccca
     2161 ggcctttcta ttgttgtcga acatctggac ggacacggac tgtggtatcc ccccttccca
     2221 aaaagaaaga cacaccttgc cctgggggta tacgatcccc ggagcccagt aggccagttc
     2281 gccgggcggg atgacgatat cgatagtcag tgttccatcc gaccccgtgg aggtcgtggt
     2341 catgaacctt ccctggtaat tataaaggac gttctccagc cgggatatca ggatgtcatt
     2401 ctgttgatag gcatggtact gggatacgac atggttgaac aggatcgcat tggtgttgat
     2461 caactgcccc ccattggtta tcgtggcatt gccgatattg acatttccgc tgaaagatgc
     2521 cggtccgttc acctggagca ggttgccgtt gtccgtcgtt gttccgagca ggaagttccc
     2581 cccgggggta agcctcgccc tcggcgtgcc gttggtatac agatccagcg gataataatc
     2641 cagggtaccc aggacacccg tggccccaaa ggcgttgcca ccctgtaccc aggactttgc
     2701 accaaggttc agcgtgtcgg atccgacgag atataagctg tcattcagtt tttgcgcgcc
     2761 tctcctgaac accgttcgcc cattgccggt attgaaaaga aagcccggaa tgttctgcgt
     2821 gtggttctcg atgatcagct ccgacgagtc acagcctgtc aatttcacac tgtcggcctt
     2881 gatcgtatac acgttctggg ccttcaagcc agcataagaa aataatagta agaataatac
     2941 gacgttccgc atagtgtttt atggttgttt tattactagt cgttttaatg catcgatctc
     3001 ctctttcagc aacccgatct ccttttcctg cgcacccatc cgcttatcct gctcggtcaa
     3061 tcttttctcc tgtgcaatat tgtataagat cagttcctct atcttcttca ccagggcagc
     3121 ctgcgtcgac ccaacatcga caccattctt ttctacctcg ccggccgacg gaaatcctgg
     3181 cagatggtgt tgctgccgga tataggactc cactcccgcc aggggcggca gccggtagcc
     3241 ggaattgaac acatagtccg gccaatcctt tgccgtcagc ctgagtttct tcgccgtgat
     3301 cgggccgttg acggccaggg aggagcgaag gacttcaccc gccgccaggg tcgaagcatc
     3361 gcggtagtag aggttgccgc cggcatcgct gaccaacacc cgggtctggg tggtatcgga
     3421 agtaagaccg ctcaaccgta cattgccggt aaggatcgag ttgccgttta aatagctgtt
     3481 tccgccaacc tgcaatttgt tcccgttatc cgcggtagcg cctaccaggg tattgccggc
     3541 ataagtaaca accaaattcg ggttcccatc gctgttgacg accgcccagt cccatccatc
     3601 atggctctca tattggaacc ctacataggg ggatgatcga taggaatatt gttcaagcat
     3661 atctaatcca ccggcaataa gtaagcgccg gttggtggtg atcagggagt cgcttaaaac
     3721 catgatctgg ttgttgttat ttccgaatgc cagttgccag ccggcgtcga aactgccgtc
     3781 cagcccgccg gatatataca atacatagcc cccggaagca tgatcaacac catataattc
     3841 ataccccgat ccgatcttta gcgaggcctg caggcgatca gtataatact ttttataaaa
     3901 aggccctcca tccatcatga tcccgccgcc ttttacgcgg aggagatagg atgcggtgtc
     3961 atcggcggtc gtcccgataa ggaaatgacc ggtattggac agtctcgcac gggccacatt
     4021 gttggtatag agatccagat gattgttgtc cagcgttccc agcaccccgg tggagccaaa
     4081 gctgttaccg ccctggaccc acgcattggg gttgagcttg agggtatccg ccccgataag
     4141 gtaatatcca tttcccagca gctgcgctcc tcttttgaag atcgttcgtc cattgccggt
     4201 attgaaaaga aaacccggga tattctgcgt atggttcccg atgatcagct ccgaagaatc
     4261 acagcccgtc agcttgacgc tatcggcctg gatcgtgtat acggattgtg cctccgtccg
     4321 gaatgaccag ccaaacaaaa gaaaaatcaa caatatcccg atccgtatat tcatatcact
     4381 tatttcgtat ctgcgttttt atcagttctt ttagcgcttc cacctctttc ttcagcgcat
     4441 cgatctccat atgctggctt tccagccgct tgtcctgatc gatcgtatac agcgtgagct
     4501 cttctatttt cttcagcagg gcgacctgtg tcccgcctac atcggcgcca tacttctcca
     4561 cttcggcggc cgaaggcagc ccaggcagat ggtgctgctg ccggatatag ctctccacac
     4621 cagcaagtcc cggcagccgg tacctgctgt c
//
LOCUS       DATGFG010000452         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1059444, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000452 DATGFG010000000
VERSION     DATGFG010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059444"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1163
                     /locus_tag="VKQ52_08380"
     CDS             48..1163
                     /locus_tag="VKQ52_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87242.1"
                     /translation="MDQPKETVAPVSTLQCSSCGATLQFAPGTHHLTCQYCGAANEIK
                     DTDNSAVVSFDYAEFIASRNGGSAAQTARVVSCKNCGASTTLPPEVNSDNCVFCASPL
                     VLSDAQTQTIIRPHYVLPFLVPQATALEDFRAWLRKLWWAPSDLTKMVSGDAHQFKGI
                     YIPHWSYDAATVSDYAGSRGDYYYVTETYTETDDDGNTVEQTREVRQTAWSPAGGSVG
                     YAFNDVLISASHSLPQEVANRLEPWKLEALVHYNEQYVSGFRSELFQIDPEKGLEEAK
                     RRMQPVIREAVVEDIGGDEQVIDYINTTYNNLGLKYILLPAWISTYKYNNKVYHFTVN
                     ACTGEVVGERPYSAAKIALAVLAAIIVIILIVMMLQK"
     gene            complement(1591..2292)
                     /locus_tag="VKQ52_08385"
     CDS             complement(1591..2292)
                     /locus_tag="VKQ52_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87243.1"
                     /translation="MQAELGLLLTAAITVACLHTVTGPDHYLPFIALSRSRGWSLTKT
                     LFWTIVCGCGHVWSSVLLGLGGAAIGWSLSKVKGLENIRGGVAGWAMLGFGLVYGIWG
                     LIRARQNRRHKHFDVYEDGSVYVYDHKHGEAVRPRDRHKVTPWVLFIVFVLGPCEPMI
                     PLLYFPAAKASWTGMLILIAVYTFFTLATMTGMVIAGFYGLHFLQTEKLERYVHALGG
                     LTLFLCGTGMVFMGW"
BASE COUNT          516 a          639 c          741 g          467 t
ORIGIN      
        1 ttctcttttt agtaatatgc cgccgccctt atcaattcct taaataaatg gatcaaccca
       61 aagaaaccgt agcgccggtt agcacgctgc aatgcagcag ttgcggcgcc acgttgcaat
      121 tcgcgcccgg tacgcaccat ctgacctgcc agtattgcgg cgccgccaac gagatcaaag
      181 acacggacaa cagcgccgta gtgtccttcg actatgccga attcatcgcc agcaggaatg
      241 gcggctcggc ggcccagacc gcccgggtcg tctcctgtaa gaactgtggc gcctccacta
      301 ccctgccgcc ggaggtgaac agtgataatt gtgtcttttg cgccagtccg ctggtgctta
      361 gcgatgccca gacccaaacc attatccggc cgcattatgt ccttcccttt ctcgtgccgc
      421 aggcaacggc gctggaggat ttccgcgcct ggctcaggaa gctttggtgg gcgccttccg
      481 atctcaccaa gatggtgagc ggcgacgcac accagttcaa gggcatctat atcccgcact
      541 ggagctatga tgccgcgacc gtttccgact atgcgggcag ccggggcgat tactactatg
      601 tcacggagac ctataccgag acggacgacg acgggaatac cgtggagcag acgcgggaag
      661 tgcggcagac cgcctggtcg ccggcggggg gttcggtggg atatgcattc aacgatgtcc
      721 tgatctccgc cagtcattcg ctgccgcagg aggtagccaa ccggctcgag ccgtggaagc
      781 tggaggcgct ggtgcattat aacgagcaat atgtgagcgg atttcggagc gagttgttcc
      841 agatcgatcc ggagaagggg ctcgaggagg ccaagcggcg gatgcagccg gtgatccggg
      901 aagccgtggt ggaggatatc ggcggtgacg agcaggtgat cgattatatc aacacgacct
      961 acaataacct ggggttgaag tatatcttac tccccgcgtg gatcagcacc tataagtaca
     1021 ataacaaggt gtaccatttt accgtcaatg cctgtaccgg ggaagtcgtc ggcgagcggc
     1081 cgtatagtgc ggcgaagatc gcgctggcgg tgcttgcggc gataattgtg atcatcctga
     1141 tcgtcatgat gctgcagaag tagcattttt aggggtttgt tcctaatgcc tggtaagttt
     1201 accgttgaag tttgggaatc ggcgtcgcaa atccttggaa aaaggagggt ttgtggtgca
     1261 tctttgtatt gttaaagcaa ccgagcaacc ctaaaccatc cgagtgaccc cagagtaacc
     1321 gagcggcctt gcgcaacccc gagcaaccct taagaaccct gagcaacctt aaacaacctg
     1381 agcaacctct gaaacgcctg accgccccct tactacctga cccctgaccg gaccctgagc
     1441 aacccagtcg ttatccaacc ctatgtgaaa accaagttat gatccaaccc tatgtgaaag
     1501 ccgaccccgc ctgtacgcaa tcgttaaggc actgagttaa ccctttacag tgacttcatt
     1561 acccgccgag accccggcgg gtttttgttt ttaccaaccc ataaagacca tgcctgttcc
     1621 gcagaggaag agggtgaggc cgccgagggc gtggacatag cgctcgagct tttccgtctg
     1681 gaggaagtgg aggccgtaga agccggcgat gaccatgccc gtcatcgtgg ccagggtgaa
     1741 aaaggtatag acggcgatga ggatcagcat acccgtccag gaggcttttg cggccgggaa
     1801 gtagagcagg gggatcatgg gttcgcaggg gccgaggacg aagacgataa acagcaccca
     1861 gggggttacc ttatgtcgat cgcggggacg gaccgcctcg ccgtgtttgt ggtcgtagac
     1921 gtagacgctg ccgtcctcgt aaacgtcgaa gtgtttgtgg cggcggttct gccgggcgcg
     1981 gatcaggccc cagatgccgt ataccaggcc aaaaccgagc atggcccagc ccgcgacgcc
     2041 cccgcggata ttttccaggc cttttacttt cgagagggac cagccgatgg cggcgccgcc
     2101 gagcccgagg aggaccgaac tccagacatg gccgcagccg cagacgatgg tccagaagag
     2161 cgtttttgtg agggaccagc cgcgggacct ggacagggcg ataaagggga ggtagtgatc
     2221 gggaccggtg actgtatgga ggcaggcgac ggtgatggcg gcggtgagga ggaggccgag
     2281 ttcagcttgc atggggggtg ggtttggagg ggaacgatga ttgggtggga gtcggcggtg
     2341 tggccgcagc tggtggggcc ggg
//
LOCUS       DATGFG010000453         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1413150, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000453 DATGFG010000000
VERSION     DATGFG010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413150"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VKQ52_08390"
     CDS             <1..1221
                     /locus_tag="VKQ52_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87244.1"
                     /translation="LPVTVPSSSELGRVTSVIAKAIKARLFLYAASPLCNGNGEYYSS
                     FKNMDGQPLMNLTYDPGKWKRAADAALDAIQAAQATGVKLYTSSLPASTPAQKAQNNY
                     RYSMVDPWNSELVWGYSMPEPFYGWQRHSMPRVSGQAYNGNSPTLKIVETYYTSNGLP
                     IDVDPGFDYANRYQISGNTIVLHQNREPRFYASIAYDRGIYQANATNMTMYFRGNEAN
                     GQSLSSPLSDYSPTGYLVQKGVHPNSIITTSQNQVINYPWPIVRLAELYLDYAEALNE
                     YDGINSQSTVLQYIDPIRIRSGIPGVAASWSLVGKTSFSQDEMRSIIRQERMIELAFE
                     GHRCWDLRRWKLGAAYLNVPVYGMNIQGATAVDFYQPAIVEKRLFNTPGYYFFPISIN
                     DLSINKNLVQNPGW"
     gene            1280..>2279
                     /locus_tag="VKQ52_08395"
     CDS             1280..>2279
                     /locus_tag="VKQ52_08395"
                     /inference="COORDINATES: protein motif:HMM:NF027705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4959 domain-containing protein"
                     /protein_id="HLZ87245.1"
                     /translation="MKKKILYTLCALLLLSLTQHCKKNNDTPTPQNVQNISVAAGYGE
                     ADFSWTFPRDSSFLYVSVSYKDSSGKTIENKFSRFTDTAIVGGLQNRPYVFTVKTVTD
                     NGVASTPQTLNVTPLQTACQIVAGSLSILPDFGSVQINWSNSTGKLVGMKIQYTDSTG
                     TTQVASASSSASRDSTSISGLKAVPGNFTVTVLDAAGNSSVPVALSATPYAEVQFVKQ
                     NWQVIDFDSQETSGEGPNNGFAKYAIDDNIKTYWHTEWQNAQPPYPHYITIDMGQPQV
                     VSRIEIVNRQGHKDGMTQIQLLGSSDNSNWTNIGTFPFQQINAGQFFPVSGAARNMR"
BASE COUNT          580 a          672 c          571 g          456 t
ORIGIN      
        1 ttacccgtaa cagtcccctc ctcctcggag ctgggccggg tcacctcggt aatcgccaaa
       61 gccatcaagg ccaggctttt tctctatgcc gcgagccccc tctgcaatgg caacggggaa
      121 tattacagta gtttcaagaa catggacgga caaccgctga tgaaccttac ttatgatccc
      181 ggcaagtgga aacgggccgc tgacgcagca ctggatgcga tccaggccgc acaggccacc
      241 ggcgtcaaac tgtacaccag cagccttccg gcttccacac cggcacagaa ggcgcaaaac
      301 aattaccggt actccatggt cgatccctgg aacagcgagc tggtatgggg ctattccatg
      361 cccgaaccct tttacggatg gcagcggcac agcatgccaa gggtatcggg gcaggcttat
      421 aatggtaatt ccccgacact taagatcgtg gaaacctatt ataccagcaa cggtctgccc
      481 atcgatgtgg accccggctt cgattatgcc aacaggtacc agatttccgg caatacgatt
      541 gtcctgcacc agaacaggga accgcggttc tatgcgtcga tcgcttatga tcgcggcatc
      601 taccaggcga atgccaccaa tatgacgatg tatttcaggg ggaacgaggc caatggtcag
      661 tcgctgagct cgccgctttc agactattca cccaccggct acctcgtgca gaaaggagtc
      721 catcccaaca gcatcatcac tacatcgcag aaccaggtca tcaattatcc atggcccatc
      781 gtaaggctcg cggaattata cctcgactat gccgaagccc tcaatgaata cgatgggatc
      841 aacagtcaat ccaccgtcct gcagtatatc gatcctatcc gtatccgttc cgggatcccc
      901 ggggtggccg cctcctggag cctggtgggc aagaccagct tttcccagga cgagatgcgg
      961 agtatcatcc ggcaggaacg gatgatcgag ctggcattcg aagggcatcg ctgctgggat
     1021 ctccgccgct ggaaactggg cgccgcctac ctgaacgtac ccgtctacgg catgaatatc
     1081 cagggagcga cagccgttga tttttaccag cctgcaatag tggagaagcg gctgttcaac
     1141 acccccggct attatttttt cccgatcagc atcaacgatc tcagcatcaa taagaacctg
     1201 gtgcagaacc ccggctggta accaatcgca cggcatgaca actccaccaa aacccggtca
     1261 ttcaataaaa acactcatga tgaaaaagaa gatactttat acgctctgcg ccttgctcct
     1321 gctatcgctg acgcaacact gtaaaaagaa caatgatact cccacgccgc agaatgtgca
     1381 aaatatcagc gttgcggccg gttacgggga agcggatttt tcctggacct tccccaggga
     1441 ttccagcttt ctgtatgtaa gcgtaagcta taaagacagc agcggcaaaa cgatcgaaaa
     1501 taaattcagc aggttcaccg ataccgctat cgtcggaggg ttgcagaacc ggccttatgt
     1561 gtttacggtg aaaacggtga ccgacaatgg cgtcgcctcc accccccaga ccctgaacgt
     1621 gacaccgctt cagacggctt gtcagatcgt ggcgggcagt ctttccatcc tgcccgattt
     1681 cgggtccgtc cagatcaact ggtccaacag cacagggaag ctggtaggaa tgaaaatcca
     1741 gtataccgac tccaccggca ccacgcaggt agcaagtgct tcctcttcgg cctcccggga
     1801 cagcacatcg atttcagggc taaaggccgt accgggaaat ttcaccgtta ccgtattgga
     1861 tgccgccggc aattcctccg ttccggtggc gctgagcgcc acaccctatg ccgaagtaca
     1921 gtttgtaaag caaaactggc aggtgatcga tttcgattcc caggaaacga gcggggaagg
     1981 cccgaataac gggttcgcca aatatgctat cgacgacaac atcaaaactt actggcatac
     2041 cgaatggcaa aatgcccaac cgccataccc tcactatatc accatcgata tgggacaacc
     2101 gcaggtggtc tcccggatcg agatcgtcaa caggcaggga cacaaggacg ggatgaccca
     2161 gatacagctc ctgggcagca gcgataacag caactggacg aatatcggca ccttcccctt
     2221 ccagcagatc aatgcagggc agttttttcc ggtaagcggg gccgcccgca acatgcgct
//
LOCUS       DATGFG010000454         7628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_698394, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000454 DATGFG010000000
VERSION     DATGFG010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698394"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1205)
                     /gene="hflX"
                     /locus_tag="VKQ52_08400"
     CDS             complement(3..1205)
                     /gene="hflX"
                     /locus_tag="VKQ52_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HLZ87246.1"
                     /translation="MIDKKNSIQKAERAVLVGLVHKTQTEEQSREYLDELAFLAETAG
                     AVTLKRFTQKLLHPDSKTFVGKGKLEEIKTFIALKEIQVVIFDDELSGSQISNIEKIL
                     DTKTIDRSDLILDIFASRAKTAQAKLQVELAQYQYLLPRLRGMWKHLERLGGGIGTRG
                     PGETEIETDRRIVKDKIALLRRRLQEIDKQAFTQRKDRGEFIRVALVGYTNVGKSTLM
                     NLLSKSEVFAENKLFATLDTTTRKVVFENTPFLLSDTVGFIRKLPHHLVESFKSTLDE
                     VREADILLHVVDVSHPGYEEQLGVVNKTLQELNAFDKPIVVIFNKIDRYEANTFDPWL
                     EEPIRTEIMNELRERWDRETNNSCVFVSATERRNIDELRRTILDKVQQLYKIRYPYRT
                     VGSWTDGQ"
     gene            complement(1290..2126)
                     /locus_tag="VKQ52_08405"
     CDS             complement(1290..2126)
                     /locus_tag="VKQ52_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ87247.1"
                     /translation="MKKYFITAMVFLATTTAALAQKKHKSPPPKTTPTDSAALVNKAL
                     QSAAANSTEQPKPKKVWDLSKRSADHFMFELGYDNWAGKPDSANISGFNHSLNFYFMM
                     DFPFKTDQRLSMGVGLGLGSSQIYFNKTYPQIAAYNNLTLTFATSAGGGSAAGADHYK
                     RFKLVTNYLEIPVELRFALDPEHMDKSWKFAVGTKIGLLLTAYTKSVDPEDVTGHVLA
                     NVVEKESSKQFFSSFKFAPTFRVSKGVIGIFGQFQVNSLLKGSAGYTVFPFSGGVVLS
                     GL"
     gene            complement(2329..3747)
                     /locus_tag="VKQ52_08410"
     CDS             complement(2329..3747)
                     /locus_tag="VKQ52_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87248.1"
                     /translation="MIAVCLLPFLLLPFLNGMAADDFHFYNLYREHGFWGAQEFIYRT
                     WAGRYTTTFLSGSVVSLDLLRHYYWMPCMGLFVCTWGAIFFLLRSLCSVLAAGLAGRQ
                     IAQISFVLFVLFLYVQADIATGFYWFSSAIVYQTSFILFLVFAGCLIKRLFIAGPAPG
                     RDALLLLLAALIIGCNEMAAVFLLLFLAVLVGILLVDRGKAPGYLWLCLGVALALCIV
                     IASTSGVIRVRHRLMNGSTSYLGVLPVVVFQFIIALFAVFKEPLFWFAGIAAFLFGVR
                     LSQEAAFAAFREKKVLVPGLALLLLPAVFGLLTVMIGSRGSLPPRALNNLTGWLAGGS
                     LALAFLAGIRNGAAMPALGTIRLSPVLLTGLLTVLLLASVNYRDAWKTVVSGYFYHAV
                     QVDRDRRLTEAGRQHEGTVTLPDYQEAEEDRIHEAFPHGVFETVHSLLLERPPLIYYY
                     DGAATRDPAYRLYYGLDSIIVR"
     gene            3922..7029
                     /locus_tag="VKQ52_08415"
     CDS             3922..7029
                     /locus_tag="VKQ52_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ87249.1"
                     /translation="MKKKTFPAFLFHPRLILFFLILCLHLASFAQPIIIKGRVKNEKG
                     EPLPGATIAVKGTTTTTVSDNEGAFSIQAPPQGNLIITAVGYAGSEEKIGGRVDLLIV
                     LHQKAAELNDVVVVGYGTQKRADLTGAVVAVTGAEMNKRIATDPTQLLQGKLPGLSVT
                     QGSGEAGNEGLVLRIRGLGTNSSAGSNPLIIVDGLPGSLSSLDPQNIESVTLLKDAAS
                     AAIYGTRAANGVILVTTKQGTSGKLQLSYDYNIGITRPTALPNNLVYNSAQYMVLWDS
                     AANHSGYPNKFTQAQIDLYSSPSRDKHLYPDYNWLHEIMRTVMVQTHHLGFTGGRNGT
                     TYNIGLGYVDQPDIMLGFSYKKYNLQFNLNSKITDNITFGSSLTLNYSQRRYASRGSQ
                     DQFLSALSQSPMYGPILPDGSGRYVNSVFPSVQTPNKNPIAIARNALVNDKDYFIQSS
                     LFLNVRIIKGLEWKTSGGFNFEYENIYDFKPVINQYNWFAGANDLPERTLDVNGQGLT
                     ETDNNTVYPVGYTQLTYARSFGSHNLKLLAGTQAEYNKSQSLAGSRNTPFSNNITQEL
                     NAGPVGSQLASGTSAEWSLRSYYGRLNYDYREKYLLEANARYDASSRFPPGNRWGLFP
                     SVSAGWVLTKEHFLEDISWLTNLKLRGSWGRLGNQNISNYPYQDLYTSADAYNTNTSY
                     AYSFSGSTLNSGVAQNSLSDPKIKWETTRIVDIGADITVFNKLSLTFDWYNKLTYDIL
                     YKPQIPAYIGLGAPTINNGRMRNTGFEASAQYADNIGPVRYTLGGNIQVNRNTLVKFG
                     APQITANNTINIEGHPFGSYYMYQWIGIFQSTDEIQRSPVQQYSPQPGYLKFKDVDGN
                     DTVNANDRVIVPGVFPKFEYSFNAGASWKNFDLAIFLYGSYGQKQFIGGWGIQPFDQG
                     SPPPKSFLNAWTPANHSTTIPLLYITGQGNASSNITSASTYYLKDASFLRIKNVQLGY
                     TLPASMAKHIAMSSLRIYFAGDNLVTFTKFPGLDPERALGNGNVRFVVHPQNQVFSFG
                     VKAVF"
     gene            7050..>7628
                     /locus_tag="VKQ52_08420"
     CDS             7050..>7628
                     /locus_tag="VKQ52_08420"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87250.1"
                     /translation="MQHPPIRSILLPLTAALMLCACSKNFLDKNPTDQLSTANFWKTQ
                     SDADQALTSIYSYLIQGYNYTSSTNTGQGWGSGTPYWETLTDNAYSGAFGTVATGDIE
                     STTGTIQSDAYNTPYKAIQDCNVFLANIGHISLDPTTMNRYLGEVRFIRGYYYFLLEQ
                     LYGDVVLSYRPLGKDSASYKLPRTPKAVVIDSI"
BASE COUNT         1874 a         2294 c         1882 g         1578 t
ORIGIN      
        1 aattattggc cgtcagtcca ggaccctacg gtccgatagg gatatctgat cttatacagc
       61 tgttgtacct tatcgaggat cgtacggcgc agctcatcga tgttgcggcg ctcggtcgcc
      121 gagacaaaga cacagctgtt attcgtctcc cgatcccagc gctcgcgcag ctcgttcatg
      181 atctccgttc ttataggctc ctccagccag ggatcgaagg tattggcttc ataccggtcg
      241 atcttattaa atatgaccac tatgggtttg tcgaaagcat tcagctcctg cagggtcttg
      301 ttgacgactc ccagctgctc ttcatagccc ggatgggaaa catcgacgac atgcaacagg
      361 atatcggcct cccgcacctc atccagcgtg ctcttaaagc tctccacgag gtgatgcggc
      421 agcttgcgga tgaacccgac cgtgtcactc agcaaaaaag gcgtattctc gaagacgacc
      481 ttccgggtcg tggtgtcgag ggtggcgaac agcttgttct ctgcaaagac ctcgctcttg
      541 ctcagcaggt tcatcagggt cgattttccg acattggtat accccaccag cgcgacccgg
      601 atgaattcgc cccggtcctt acgctgggtg aaggcctgct tgtcaatctc ctgcaaccgg
      661 cggcgaagca gcgcgatctt atcctttacg atccggcggt cggtctcgat ctcggtctca
      721 cccggacccc gggtgccgat gccgcccccc agccgctcaa ggtgtttcca catcccccgc
      781 aacctgggta gaagatattg gtactgagcc agttctacct ggagtttggc ctgtgccgtc
      841 ttggcgcggc tcgcgaagat gtcaagaata agatccgatc gatcaattgt ttttgtatct
      901 aatatttttt caatattgga gatctgggaa cccgagagct catcgtcaaa aatgaccacc
      961 tggatctctt ttagcgcgat aaacgtcttg atctcctcca atttaccctt cccgacaaag
     1021 gtcttgctgt ccgggtgcaa cagcttctgg gtaaaccgct ttaacgtgac tgccccggcc
     1081 gtttccgcca ggaatgccag ctcatccagg tattcccggg actgttcctc cgtctgcgtc
     1141 ttgtgtacca aaccgaccag caccgctctc tccgcctttt ggatactatt tttcttatct
     1201 atcaaactat tatgattagg acggcaaagg taaaataaaa aatccgccag acagagccgg
     1261 cggatcaata tcattgccac aagcagtatt tacagcccac tcagcaccac acccccggaa
     1321 aaagggaata ccgtatatcc ggcacttccc ttgagcagac tgttgacctg gaactgaccg
     1381 aagatcccga taacaccctt gctgacgcgg aacgtcgggg caaatttgaa gctgctgaag
     1441 aattgtttgg aggactcttt ttcgacaaca ttagccagaa catggccggt gacgtcttcc
     1501 ggatcgacgg acttggtata ggcagttagc agcaagccga tcttggtacc gaccgcaaac
     1561 ttccagctct tgtccatatg ctcgggatcg agtgcgaacc gtaattcgac cggaatttca
     1621 agatagttgg tgaccagctt gaaccgcttg tagtgatccg ctcctgcggc gctcccgcct
     1681 cctgcggagg tcgcgaatgt cagcgttagg ttgttgtagg cggctatctg tgggtaggtt
     1741 ttattgaagt agatctggct gctcccaaga cccaaaccga cacccatact cagacgttgg
     1801 tcggttttga aggggaagtc catcatgaaa tagaagttaa ggctatggtt gaagccactg
     1861 atattggcgc tgtccggctt acccgcccag ttatcataac ccaattcgaa cataaaatgg
     1921 tcggccgacc gcttgctgag atcccagacc ttcttgggtt ttggctgttc cgtgctatta
     1981 gccgcggctg attgcagggc cttattgacg agtgcggcgg agtcggttgg agttgtctta
     2041 gggggtgggg atttgtgctt tttttgagcg agagcagcag ttgtggtggc taaaaaaacc
     2101 atcgcagtga tgaaatactt cttcataatt tcacttgtag gtgatgtctt ttggtgatct
     2161 gaaagcaaac acgagggctt tttccgaggt ttttaggccc atccgggttc tttttgatct
     2221 ggttgacaaa tgtattctcc ttggggttaa taaaaggtta aagaaccccc ttcaggcctg
     2281 atttttaatc ggccgcaaga tattactttt tcaccaaatc ggacggggtc accttacaat
     2341 gatggagtcg agaccatagt aaagccggta cgccgggtcc cgcgtcgcag ccccgtcata
     2401 gtaatagatc agcggcggcc tttccagcag caggctatgc accgtctcga agacgccatg
     2461 gggaaatgcc tcgtggatcc gatcctcctc ggcctcctgg tagtcgggca gggtaacggt
     2521 cccctcatgc tgcctgccgg cttcggtcaa tcgccggtcg cgatccacct ggacggcatg
     2581 gtaaaaataa cccgaaacga ctgtcttcca ggcatcgcgg tagttgacgc tcgccaacag
     2641 taaaacggtc agtaatccgg tcaggagtac cggggagagc ctgatcgtcc ccaacgccgg
     2701 catcgcggcc ccattacgga tacccgccag gaatgccagg gcaagagatc caccggcgag
     2761 ccagccggtc agattattca gcgcccgggg tgggagcgag cccctggacc cgatcatcac
     2821 cgtcaggaga ccgaacacag ccggcagcag gagcagggcc agcccgggaa ccaacacctt
     2881 cttttcccgg aaagcagcaa aagcagcctc ttgtgaaagc cggacgccaa agagaaaagc
     2941 ggcgatacca gcgaaccaga acagaggctc tttgaaaacg gcaaagaggg cgatgataaa
     3001 ttgaaagacc accaccggca gaacaccgag gtaagaagta ctgccattca tcagccggtg
     3061 ccgcacccgg atgacccctg aagtcgacgc aataacgata cacagggcca gggctacacc
     3121 caggcaaagc cacagataac ccggcgcctt cccgcggtca accagcagga tacccacaag
     3181 cacggctagg aaaagaagaa gaaatacagc cgccatctcg ttgcatccga tgatgagcgc
     3241 cgccagcaga agcagcaggg catcacgccc gggcgcaggg ccggcgatga atagccgttt
     3301 gatcaggcag cccgcaaaca caagaaagag aataaaagaa gtctgataga cgatagcgct
     3361 gctgaaccag taaaagccgg tggcgatatc cgcctgcaca tatagaaaca gcacaaacag
     3421 cacaaagctg atctgcgcga tctggcgccc cgccaaacct gccgccaaca ccgaacacaa
     3481 actcctcaac aaaaaaaaga tcgcgcccca ggtacaaaca aaaagcccca tacaaggcat
     3541 ccagtaataa tgccgcagca gatccaggct cacgacggag ccactcaaaa aagtagttgt
     3601 ataccgacct gcccatgtac gatagataaa ctcctgtgcg ccccaaaaac catgttcccg
     3661 gtacaggttg taaaaatgga aatcatccgc cgccatgccg ttgagaaagg gaagaaggag
     3721 aaaagggagc aggcaaactg cgatcaccag ccaaaagaaa cccgtaagcc tgttttttcc
     3781 cgcagttgaa ttacccatcg atcggaaaag tcctcccggc taaaaatacg catttacgcc
     3841 aaaaggagat aataatttga caatcttgcg gaattcaccg gaaggaaata gtccgtactt
     3901 gccctcacta attgctgcta catgaaaaag aaaaccttcc ctgcatttct cttccatccc
     3961 cgcttgatcc tcttcttcct cattctttgt ctgcatctcg cctcctttgc ccagcccatc
     4021 atcataaaag gcagggtaaa gaacgagaaa ggcgaacccc tacccggcgc caccatcgcc
     4081 gtaaagggga cgaccactac cacggtctcg gataacgaag gggcattctc catccaggcc
     4141 ccgccccagg gcaacctgat catcacggca gtcggatatg ccggcagcga ggaaaagatc
     4201 ggcggccgcg tcgacctcct catcgttctg catcaaaagg cggccgagct caacgatgtg
     4261 gtagtggtgg gctatggcac ccagaaaaga gccgacctca ccggcgctgt tgtcgccgtc
     4321 accggcgccg aaatgaataa acggatcgcc acagacccca cccagctgct ccagggcaag
     4381 ctccccggtc tctccgtgac ccagggctcc ggggaggcag gcaacgaagg cctggtgctc
     4441 cggatcaggg gcctcggcac caacagctcg gccggcagca acccgttgat catcgtagat
     4501 ggtcttcccg gcagcctgtc ttcgctggac ccccagaaca tcgagtcggt gaccctcctg
     4561 aaagacgcgg cttctgcggc catctatggt acccgcgccg ccaacggggt catcctggta
     4621 acgacaaaac aggggacgag cggcaaattg cagttgtctt atgattataa catcgggatc
     4681 acccgcccga cggccttgcc caataacctc gtctataatt cggcgcagta tatggtcctg
     4741 tgggactcgg cggccaacca ttcgggttac cccaataaat tcacccaggc ccagatcgat
     4801 ctgtacagta gccccagccg cgacaaacat ctctatcccg attacaactg gctccatgag
     4861 atcatgcgca ccgtcatggt gcagacccat cacctggggt tcaccggcgg caggaacggc
     4921 accacctaca atataggact cggctatgtc gaccagccgg acatcatgct gggattctcg
     4981 tataagaaat acaaccttca gttcaatctg aattcgaaga tcacggacaa tatcaccttc
     5041 ggttccagcc tcaccctcaa ttacagccag cgacgctatg cctcccgcgg cagccaggac
     5101 cagttccttt ccgccctttc ccagtcgccc atgtacggcc cgatactgcc ggacggcagc
     5161 ggccggtatg tgaattccgt cttccccagc gtacagacac ccaacaagaa ccccatcgcc
     5221 atcgcccgga atgccctggt caacgacaag gactatttta tccaaagcag cctgttcctg
     5281 aacgtcagga tcatcaaagg gctcgagtgg aaaacaagtg gtgggtttaa tttcgaatac
     5341 gagaacatct atgatttcaa accggtgatc aatcaataca actggttcgc aggtgctaac
     5401 gatcttcccg aaagaacact cgatgtcaat ggccagggac tcaccgaaac ggataacaat
     5461 acggtatacc cggtcggtta tacccaactc acctatgccc gtagcttcgg ttcccacaac
     5521 ctcaaactgc tcgcgggcac gcaggccgag tacaacaaat cccagtccct ggccggctcc
     5581 cgcaataccc ccttttccaa caatattacc caggaactca acgcgggtcc cgtaggctcc
     5641 cagctggctt ccggcacatc agcggaatgg tccctgcgtt cgtattacgg aaggctgaac
     5701 tatgactacc gggagaaata cctgctcgag gcaaacgcgc gttacgatgc gtcttcccgc
     5761 tttcccccgg gtaaccgctg gggcctcttc ccatcggtgt ccgccggctg ggtcctgaca
     5821 aaagaacatt tcctggaaga catctcctgg ctcaccaacc tgaagctccg gggctcctgg
     5881 ggaagactgg ggaaccagaa catctccaac tatccctacc aggacctcta caccagcgcc
     5941 gacgcctata ataccaacac ctcctatgct tattctttct ccggcagcac cctgaactcg
     6001 ggtgtagccc aaaactccct ctccgaccca aagatcaaat gggagacgac ccgcatcgtc
     6061 gatatcggcg ccgatataac ggtattcaac aaactaagcc tcaccttcga ctggtacaac
     6121 aagctcacct acgatatcct ctacaaaccc cagatcccgg cctatatcgg gcttggcgca
     6181 ccgactatca acaacggccg gatgcggaat acgggcttcg aggcttcggc gcaatatgcc
     6241 gataacatcg gccccgttcg ctataccctg ggcggcaata tccaggtgaa ccggaatacc
     6301 cttgtgaagt tcggcgcccc gcagatcacc gccaacaaca cgatcaatat cgaagggcat
     6361 ccgttcggca gctactatat gtaccagtgg atcggcatct ttcagtcgac cgacgagatc
     6421 cagcggtccc ctgtccagca gtatagcccg cagccgggat acctgaagtt caaggatgtc
     6481 gacggcaacg acaccgtcaa tgccaacgac agggtgatcg ttcccggggt cttcccgaaa
     6541 tttgaatact cctttaacgc cggcgcctca tggaagaact tcgatctcgc gatcttcctc
     6601 tatgggtcct atggccagaa acaattcatc ggcggctggg gcatccaacc cttcgaccag
     6661 ggctccccgc ctccaaagag ctttctcaat gcgtggaccc cggctaacca tagcaccacg
     6721 atcccgcttc tctacatcac cggtcagggg aacgccagca gcaatatcac cagcgcatcc
     6781 acctattatc taaaagacgc gtccttcctc cggatcaaga acgtgcagct cggctacacc
     6841 ctgcccgcct cgatggcaaa acatatcgcg atgagcagct tgcggatcta ttttgcgggt
     6901 gacaacctgg tcacgtttac aaaattcccg ggtctcgatc ccgaacgcgc actcggcaac
     6961 ggtaacgtcc gcttcgtcgt gcacccgcaa aaccaggtat tttcctttgg cgtaaaagcc
     7021 gttttctaaa tcgaaaaaat tgagatcaca tgcaacaccc acctataaga tcgatccttc
     7081 ttcctctcac cgccgccctg atgctatgcg catgcagcaa gaattttctc gacaagaacc
     7141 cgacagacca gctctccacg gccaatttct ggaagaccca gtcggatgcc gaccaggcgc
     7201 tgacctctat ctacagctac ctgatccagg gatataacta taccagcagc accaatacgg
     7261 ggcagggctg ggggtcgggt accccttact gggagacgct caccgacaac gcctatagtg
     7321 gcgcctttgg gacggtggcg acgggggata tcgaatcgac caccggcacg atacaatccg
     7381 atgcctacaa caccccttac aaggcgatcc aggactgcaa tgtcttcctg gccaatatcg
     7441 gccatatctc tctggacccg acgaccatga accgctacct cggggaggta cggtttatcc
     7501 gcggctatta ttatttcctg ctcgagcagc tatatggcga cgtcgtcctg agctaccgcc
     7561 ccctgggcaa ggactcggcc agctacaaac tgcccaggac gccaaaggcg gtcgtcatcg
     7621 attccatc
//
LOCUS       DATGFG010000455         5822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1205620, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000455 DATGFG010000000
VERSION     DATGFG010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205620"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VKQ52_08425"
     CDS             <1..582
                     /locus_tag="VKQ52_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLZ87251.1"
                     /translation="KGMKDFIMITLGTGVGSGIVANGKLIYGHDGFAGELGHTIIIPN
                     GRYHEGTGKKGSLESYASATGVKLTAIEMLEKSEEFSLLRNTPKERLDSRVVYDAAIQ
                     GDKLAKDIFDFTGSVLGMALANFVMFSSPEAIILFGGLTKAGDLILKPTREHMEENLI
                     QVFQNKVKILVSHLKESDAAILGASALVWDMKG"
     gene            complement(637..1596)
                     /locus_tag="VKQ52_08430"
     CDS             complement(637..1596)
                     /locus_tag="VKQ52_08430"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HLZ87252.1"
                     /translation="MPKPVAFCLLYLLSFRPCLGQLRWQRVDSAYGPLPSSMHVYRST
                     DSLDGFPSVAYYVSARLKDKNIQFTATTGQGKRFTPQQYYQIEQFPLLVVNCTFFSFA
                     NNQNFNLVVKDGKMQAYNVTSLRGTGNDSLLFYYPTRGAIGIDRKRRADVAWTFTDSS
                     RRWPYAFEEMPVIARGTDTVPSIYDLNDIEWKWWKMRTAVGGGPVLIHDGKIHITDVE
                     EQLFPGSNRNGERLPRTAMGYTRDGRLIILVVQGRAPGVAAGATLQQEAQLLYRLGCY
                     EALNLDGGGSTCMLVNGRETIRPSDRDGERPVPAVFLIKRTKQ"
     gene            complement(1608..2543)
                     /gene="lipA"
                     /locus_tag="VKQ52_08435"
     CDS             complement(1608..2543)
                     /gene="lipA"
                     /locus_tag="VKQ52_08435"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HLZ87253.1"
                     /translation="MESNVNASACLPAGESKIQKPNWLRVKLPIGENYKHVRGLVDTH
                     KLHTICESGNCPNMGECWGEGTATFMILGNICTRSCGFCAVATGRPEPVDWDEPQRVA
                     EAIYLMKVKHAVITSVDRDELKDGGSIIWQNTIRAVKALNPETTLETLIPDFKAEQEN
                     IARVIEAAPEVVSHNIETVERLTRQVRIQARYWRSMETLKVLKEGGMRTKSGIMLGLG
                     ESKEEVVQTMTDLRNSGVDVITLGQYLQPTKKHLPVVRFVHPDEFAGLREIGYNLGFD
                     YVESGPLVRSSYHSERHVIPGYGRGEWLRGKEAAL"
     gene            complement(2594..3406)
                     /locus_tag="VKQ52_08440"
     CDS             complement(2594..3406)
                     /locus_tag="VKQ52_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87254.1"
                     /translation="MSRLFLTILASTLVLNLFAQNADSVTMVSKRNRQNSKRDRMNNL
                     LRMEEEGDLIFNKHNIFGIRLATDGYGINFEKGKFKSPTKTLLFQFELNEKKDPKEHH
                     ISATSDGINFSSVVPYKLNNLYEFKMAIGEQRLIGGKGNRNGVAVTALYSGGVTVGML
                     KPYYLDIQNQITGATTRKTYAQMATDTTQGDLITGASGFTVGWGNLSIKPAVNARQAM
                     RFDYGRLNQTVAAIEVGLTEEYYFSKIPLVYLIPQHQFFFNAYVAILFGSRK"
     gene            3458..3883
                     /locus_tag="VKQ52_08445"
     CDS             3458..3883
                     /locus_tag="VKQ52_08445"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLZ87255.1"
                     /translation="MTSTVIYEGDLRTVAQHLQSGTEIETDAPTDNQGKGERFSPTDL
                     VATALGACMATTMGIKARDLQVDLRGMKLSIQKIMKPDPRRIAGVNIVFDFPGELVVD
                     EKQKTILERTAHTCPVMYSIHPDMEVKIEFNWNTVPATA"
     gene            complement(4061..5224)
                     /locus_tag="VKQ52_08450"
     CDS             complement(4061..5224)
                     /locus_tag="VKQ52_08450"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HLZ87256.1"
                     /translation="MREELLHFIWRFRHFNQQELVTEAGEPLQILFPGEPHRDQGPDF
                     RNARVRIGGVCYVGPVELHVLASDWVRHAHDGDVHYRDVILHVVWENDLRAEQGKMPV
                     LVLQSRVAGWLLSRYEHWMRHPSFVPCERQLAGVDAAVRAGWMRQLALRRLQRRAVAV
                     YDRLEENRQDWEETGWWLMARSMGLPVNAGVFEAVARSLPLRVLRRQGAYLPALEGLL
                     LGQAGLLPAADARMREYQFLRVKYGLRPVGSPVSFLRMRPAHSPLERLVQLAALLADG
                     RGWFAFIRDAETPEVVSAAMEGVRGLGAEMRRGILINAFIPLLFAYGQREKAMRWLSG
                     MKAERNVILRGWEERGMAAKDAAGSQALLELKKEYCNARRCLECAIGRALLAA"
     gene            complement(5264..>5822)
                     /locus_tag="VKQ52_08455"
     CDS             complement(5264..>5822)
                     /locus_tag="VKQ52_08455"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HLZ87257.1"
                     /translation="RIVLASGSPRRKQLLEWAEVDFEVLVKDTPETWPAGLATADVPV
                     HIARNKALAVKREVAPGRVVIAADTVVVLGEEIIGKPRDRAEAVNILSRLAGHRHEVI
                     TGVVLLRDGEEQAFADVTSVWFHPLSLPEIEGYVDRYQPYDKAGAYAIQEWIGVVGIK
                     GIEGDFYNVMGLPVSRVVQALRKWQ"
BASE COUNT         1268 a         1968 c         1433 g         1153 t
ORIGIN      
        1 aaaggcatga aggactttat catgatcacc ctcggcaccg gcgtcggcag cggcatcgtc
       61 gccaacggaa aactgatcta tggccacgac ggcttcgccg gcgaactggg acataccatc
      121 atcatcccca acggccgtta ccacgaaggc acgggaaaga aaggctccct ggaaagctat
      181 gcctccgcca ccggcgttaa actcaccgcc atcgagatgc tcgaaaaaag cgaggagttc
      241 agcctgctgc ggaatactcc aaaagaacgc ctcgactccc gcgtcgttta tgacgccgcc
      301 atccaggggg ataaactggc aaaggatata ttcgatttca ccggctccgt cctcggcatg
      361 gcgctcgcca atttcgtcat gttctccagc cccgaagcca tcatcctctt cggcgggctc
      421 accaaggccg gcgatctcat cctgaaaccg acccgcgaac acatggagga aaaccttatc
      481 caggtgttcc agaacaaggt aaagatcctc gtcagccacc tgaaggaatc cgacgccgcg
      541 atcctcggcg ccagcgcact ggtctgggat atgaaagggt gatgaggcca tgccggtctt
      601 tcggatgcag ccttattgca ttgttcggat acagccttat tgcttcgttc gcttgatcag
      661 gaaaaccgcg ggcaccggcc gctctccgtc cctgtcggaa gggcgtatcg tttccctgcc
      721 gttcaccagc atgcaggtac tgccgccccc atcgagattc aacgcctcat agcacccaag
      781 acgatacaac agctgcgcct cctgctgcaa agtggcgccc gccgccaccc ccggcgcccg
      841 gccctgcacc accaggatga tcagccgccc gtcccgcgta taccccatgg ccgtccgcgg
      901 aagccgctcg ccattccggt tgctgccggg aaacagctgc tcctcgacat cggtgatatg
      961 gatcttcccg tcatggatca acaccgggcc gcctcccacc gccgtccgca tcttccacca
     1021 cttccactcg atatcgttca gatcatagat cgacggaacg gtatccgtac ccctcgcgat
     1081 caccggcatc tcctcgaagg catagggcca gcgcctcgaa ctgtccgtaa acgtccaggc
     1141 gacatccgcg cgccgcttcc ggtcgatgcc gatcgcgccc cgcgtgggat aatagaacaa
     1201 aagcgaatca ttgcccgtcc cccggagaga ggtgacatta tacgcctgca tcttcccgtc
     1261 ctttacgacg agattgaaat tctgattatt ggcaaaggaa aaaaaagtac aattcaccac
     1321 cagtaagggg aactgttcga tctggtagta ttgctgaggt gtaaagcgct taccctgtcc
     1381 cgttgtcgcc gtgaactgga tgttcttatc cttcagccgg gcggagacat aataggcgac
     1441 agagggaaaa ccatcgagac tgtcggtact ccggtagaca tgcatcgaag aaggaagcgg
     1501 cccataagcg gagtccaccc gctgccagcg caactgcccc aggcagggcc ggaaggacag
     1561 caggtacaac aggcagaacg caaccggttt gggcatggca ggaattgtta taaagcagct
     1621 tctttccccc gcagccactc cccgcgacca tacccgggga tgacatgacg ttcggaatga
     1681 taggaactgc gcaccagcgg accactttcc acatagtcga atcccaggtt gtacccgatc
     1741 tcccgcagcc cggcaaattc gtcgggatgg acgaaccgca ccacaggcaa atgcttcttc
     1801 gtcggctgca ggtattgccc aagggtaatg acatcgacgc cgctgttgcg cagatcggtc
     1861 atggtctgta ccacctcttc cttcgattca ccaagcccca gcatgatccc gctcttggtc
     1921 cgcatccccc cttctttcag caccttgagg gtctccatgc tgcgccagta cctggcctgg
     1981 atccgcacct gccgcgtcag ccgttccact gtctcgatat tgtgcgaaac cacctcgggc
     2041 gcagcctcga taacccgggc gatattctcc tgctccgcct tgaaatcggg gatcagcgtt
     2101 tccagtgtcg tttcgggatt caacgccttc acggccctga tcgtattctg ccagatgatg
     2161 cttcccccat cctttaactc gtcccggtca accgacgtga tcaccgcatg ctttaccttc
     2221 atcaggtaaa tggcttcggc aacccgctgc ggctcgtccc agtccaccgg ctccggccgg
     2281 cccgtcgcca ccgcacaaaa accacagctg cgggtgcaga tattgccaag gatcataaag
     2341 gtggccgtcc cttcccccca gcattcaccc atattggggc agttgccgct ctcacagatg
     2401 gtatgaagtt tatgggtatc taccagcccc cgaacgtgct tgtagttttc gccgatcggc
     2461 aattttacac gtaaccaatt gggcttctgt atcttagatt ctcctgccgg gaggcaggcc
     2521 gatgcattta cgttggattc catccaacaa atgtacggca gtttccacta acaaatgtcg
     2581 gcccgccgac ccatcatttt cgactcccaa aaaggatggc gacataggca ttgaagaaga
     2641 actggtgctg tgggatgagg taaacaagcg gtatcttgga gaaatagtac tcttccgtca
     2701 agcccacctc gatcgccgca acggtctggt tcagccggcc atagtcgaac cgcatcgcct
     2761 gccgggcatt caccgccggc ttgatcgaca ggttgcccca tcctaccgta aaccccgatg
     2821 cgccggtgat caggtcacct tgcgtcgtat ccgttgccat ctgggcatag gtcttacgcg
     2881 tcgtggcccc cgtgatctgg ttctggatat cgagatagta gggctttagc atcccgaccg
     2941 tgaccccgcc ggaataaagc gccgtcaccg ccaccccatt ccggttaccc ttgccgccga
     3001 taagccgctg ttccccaatg gccatcttga actcatagag gttattgagc ttatagggaa
     3061 ccaccgagga gaagttgatg ccgtccgagg tcgcggagat atgatgttcc ttcgggtcct
     3121 tcttctcgtt cagctcgaac tgaaaaagca gggtcttcgt cgggcttttg aatttcccct
     3181 tctcgaaatt gatcccatag ccgtcggtgg ccagcctgat cccgaatata ttatgcttat
     3241 taaagataag atccccttcc tcttccatcc tcagcaggtt gttcattcga tcccttttgg
     3301 aattctgcct gttgcgtttc gacaccatag tgacgctgtc cgcattttgc gcaaaaagat
     3361 tcagcaccag ggtcgaggcg agaatagtca ggaaaagtct gctcactttt aatgatttaa
     3421 tttaactggc gtaaatttag gcaaaaatta ttgcacaatg acttcaacag ttatttacga
     3481 aggagattta cgaacggttg cccaacacct gcaatccggg acagaaatag agaccgacgc
     3541 acccaccgac aaccagggga aaggtgagcg tttctcccct accgacctcg tagctacggc
     3601 cctcggcgcc tgtatggcta ccaccatggg gataaaggcc cgcgatctgc aggtagacct
     3661 ccgcggcatg aaactgtcca tccagaagat catgaagccc gatccgcgca ggatcgccgg
     3721 cgtcaatatc gtcttcgact tccccggcga actcgtcgta gatgaaaaac aaaagaccat
     3781 tctcgagcgg accgcccata cctgccccgt catgtacagt atccatcccg atatggaggt
     3841 aaagatcgag ttcaactgga ataccgtacc ggcgacggcc taggcggccc aaagcggacc
     3901 accggttcgc tggccgaagg cccaacataa gccgttggcc gccgaacggg taaatttccc
     3961 aagacccgtt caggcggccc aaagcggacc accggttcgc tggccgaagg cccatcatta
     4021 gcagtgtcgc ttaaccgaga taaatttccc gacatctcgg ttaagcggcc aataacgccc
     4081 ggccgatcgc gcattcgagg catcgccggg cgttgcaata ttcctttttc agctccagca
     4141 gcgcctgcga gccggccgca tccttcgccg ccatcccccg ttcctcccat ccgcgcagga
     4201 tgacattccg ctcggccttc atcccgctca gccagcgcat cgccttctcc cgctgcccat
     4261 aggcgaacaa aagcgggata aaggcattga tcaggattcc gcgccgcatc tccgccccca
     4321 gtccccgcac cccctccatt gccgcgctca ccacctcggg ggtctctgca tcccggataa
     4381 aggcaaacca gccccttccg tccgccagca acgccgccaa ctgcaccagc cgttccaagg
     4441 gggaatgagc tggccgcatc cggagaaacg acaccggcga cccgacgggt ctcagcccat
     4501 atttcacccg taagaactga tattcccgca tccgggcatc cgcggccgga agcaaccccg
     4561 cctgccccag caacagcccc tccagcgcgg gtagataagc tccctgccgc ctcagcaccc
     4621 gcaatggcag gctcctcgcc accgcctcga agacccccgc attcaccggc agacccatgc
     4681 tccgcgccat cagccaccag cccgtctcct cccagtcctg ccggttctcc tccaggcgat
     4741 catacacggc aaccgcccgg cgctgcaatc gccgcaacgc cagctgccgc atccaacccg
     4801 cccggacagc cgcatccacg cccgccaact gccgctcaca ggggacaaag gacggatgcc
     4861 gcatccagtg ctcgtaccgg ctcagcaacc acccggccac cctcgactgc aacaccagca
     4921 ccggcatctt cccctgctcc gcccgcaggt cgttctccca gaccacatgc aggatgacat
     4981 cccggtaatg cacatcgccg tcatgcgcat gccgcaccca atccgaggcg agcacatgca
     5041 gctccaccgg tccgacataa catacccctc cgatgcgcac tcgcgcattc cggaaatcag
     5101 gaccctggtc ccggtgtggc tcacccggga aaaggatctg cagcggctcc cccgcttcgg
     5161 ttaccagttc ctgttgatta aaatggcgga accgccagat gaaatgaagc aactcttccc
     5221 gcataaccca cctcctcttt tattattatt gccatttgcg caactactgc catttgcgca
     5281 aggcctgcac caccctgctg acgggcaggc ccatcacgtt ataaaaatct ccttcgatgc
     5341 cctttatccc taccaccccg atccactcct ggatggcata ggccccggcc ttgtcataag
     5401 gctgatagcg atcgacatat ccttcgatct ccggcagcga caagggatgg aaccacaccg
     5461 acgtgacatc ggcaaaggcc tgttcctcgc catcccgtaa caagaccacc ccggtgatca
     5521 cctcgtgccg gtgacccgcc agcctcgaca ggatattcac cgcctcggcc cggtccctgg
     5581 gcttgccgat gatctcctcc cccagcacca ccaccgtatc ggcggcgatg accacccggc
     5641 cgggcgccac ctcccgcttc acggcaagcg ccttgttgcg cgcgatatgg acagggacat
     5701 ccgccgtcgc aagacccgcc ggccaggtct ccggggtatc ctttaccagc acctcaaaat
     5761 cgacttccgc ccattccaac aattgcttgc gccggggcga gccggacgcc aatacgatac
     5821 gt
//
LOCUS       DATGFG010000456         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1035094, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000456 DATGFG010000000
VERSION     DATGFG010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035094"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1004
                     /locus_tag="VKQ52_08460"
     CDS             315..1004
                     /locus_tag="VKQ52_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HLZ87258.1"
                     /translation="MVLAILAFTWTACSKAGSTSTTSAVTYLSMIHGAPYTGAATLYL
                     NDTLITQSTGIATGAFSSKYGTIRPGSYTAKLKKAGTDSLLDQLTASSYDTLNFYTIL
                     LYNSPSDKSAHAVKIVDDFSSVTSSNAYYRFFHLAPDYPRVNLYLNGTLMQDNRTPAD
                     NAVINDFNAFKPISAGYYTITVKDATTDSVIATSPSVSLAAGTPYTIWVGGLKSTNNT
                     NMSINVLQAAY"
     gene            complement(1021..>3560)
                     /gene="gcvP"
                     /locus_tag="VKQ52_08465"
     CDS             complement(1021..>3560)
                     /gene="gcvP"
                     /locus_tag="VKQ52_08465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HLZ87259.1"
                     /translation="ADLTGLPLANASLLDEGTAAAEAMTMFFNLKNKDHDHITTPRFF
                     VDGNIFAQTKDLLLTRALPIGIELVVGDYRKAVIDGSYFGAIVQYPASDGSVNDHSAF
                     IQQVHNAGAYVVMATDLLALTLLTPPGELGADVAVGSAQRFGVPMGFGGPHAAFFTAK
                     DEFKRSIPGRIIGVSVDAQGNRALRMALQTREQHIKREKATSNICTAQALLANMSAMY
                     AVYHGAEGLTRIAKRVSLLARTLATELEAMGYPNLNEYYFDTVRVKVKDPVALRQAAE
                     THGMNFFYSGGGTVGITLDETTAQEDVVNILSVFGHLTGTSLVSATFDVDNTLANIPT
                     SVTRVTEFLTHPVFNSHHSETKMMRYLRSLENKDLSLNTSMISLGSCTMKLNAATELI
                     PVSWPEFGGLHPFAPADQWKGYRRILTELEEWLSEITGFAATSLQPNSGANGEYAGLL
                     TIRAYHKAHNGSHRDVMLIPISAHGTNPASAIMAGMKVVVVKSDDEGHIDVEDLKEKA
                     IQYKDSLAGLMVTYPSTHGVFEESIKEICRTIHENGGLVYMDGANMNAQVGLTSPGLI
                     GADVCHLNLHKTFAIPHGGGGPGMGPICVTAQLKPYLPGHVQSTDAERRAAGNGQAVT
                     GAAGHAVSAAPFGSASILLISYAYIKMLGADGVTDATRYAILNANYMKARLEKYYKIL
                     YSGANGTCAHEFIVDLRPFKASAGVEAEDVAKRLMDYGFHAPTLSFPVPGTIMIEPTE
                     SEDKAELDRFCDALISIHEEIRAIEEGKADKTDNPLKNAPHTQQVIIAEEWKHAYTRN
                     EAAFPLPYTRENKFWPSVARVNNTYGDRNLICTCEPVSSYAEEAIAN"
BASE COUNT          707 a         1012 c         1050 g          791 t
ORIGIN      
        1 gaagtcaaac tgacccccgg gaagcatgcg ctgaccctgc agttcgccga cggtatccac
       61 cggagctatg gcgccgcgct ggccacgacg atccatgtta cggtgaaata gcatatcttt
      121 ataatacgat tgggccggcc ccgcttatgc tcatgggccg gccttttttt gggtggatgg
      181 agtgcgctca agacaacccg cgcaacgaaa aagccgtaag tgttgtcatg gagtaagatc
      241 cactgtagct tgagttacag cttgaaaaaa tgacatcaat gagaaaaagc acaagaaatt
      301 ttggctgttt tgttatggtt ttggccatcc tggcatttac ctggaccgcc tgctccaagg
      361 ctggttctac gtcgacgaca agcgcggtta cctatttatc gatgatccac ggcgcaccct
      421 ataccggcgc cgccacgctt tacctgaatg atacccttat tacccagtcc accggtatcg
      481 ccaccggggc cttttcgtcc aaatatggca ccatccggcc ggggtcctat accgcaaagc
      541 tcaaaaaggc cggtaccgac agtctcctcg accagctcac cgcctccagt tatgataccc
      601 tgaatttcta tacgatcctg ctgtataaca gccccagtga caaatcagcg catgccgtga
      661 agatcgtgga tgatttcagc tcggtcacct cgtcgaatgc gtattaccgt ttcttccacc
      721 tggcgccgga ctatcccagg gtcaacctct acctgaacgg cacacttatg caggacaacc
      781 gcaccccggc cgataatgcc gtcatcaatg acttcaatgc cttcaagccg atatccgccg
      841 gatattatac catcaccgtc aaggacgcca ccacggacag cgttatcgcc acctccccct
      901 ccgtcagcct cgcggccgga actccctata ccatctgggt gggcgggttg aaatcgacga
      961 ataataccaa catgtcgatc aacgtgttgc aggcagcata ttaaattaag ctgaacctgc
     1021 ctaattcgct attgcttctt ccgcatagga actcaccggt tcgcaggtgc agatcaggtt
     1081 gcggtcgcca taggtattgt tgacccgggc gacactgggc cagaacttgt tctccctcgt
     1141 atagggcagg gggaaggcgg cctcgttgcg cgtgtaggca tgtttccatt cttcggcgat
     1201 gatcacctgc tgggtgtgcg gcgcattctt caggggattg tccgttttgt ctgccttccc
     1261 ttcttcgatg gcgcgaatct cttcatggat gctgatcagg gcatcgcaga accggtccag
     1321 ttcggcctta tcctcgcttt ccgtcggctc gatcatgatc gtgcccggta cggggaagct
     1381 gagggtgggg gcgtggaagc cgtagtccat caggcgcttg gccacgtctt ccgcttcgac
     1441 cccggcgctg gccttgaagg ggcgcaggtc gacgatgaat tcgtgggcac aggtgccatt
     1501 ggcgccgctg tagaggatct tataatactt ttccagccgg gccttcatat agttggcgtt
     1561 caggatggca tagcgggtgg cgtcggtgac tccatcggcg ccgagcatct tgatataggc
     1621 atagctgatc agcaggatgc tggccgaacc gaagggggcg gccgatacgg cgtgaccggc
     1681 ggcgcccgtc acggcctggc cattacccgc cgcacggcgt tcggcgtccg tgctctggac
     1741 atgcccggga agatagggct tcagttgtgc cgttacacag atgggcccca tgccgggacc
     1801 gccgccgcca tgcgggatgg cgaaggtctt gtgcaggttg agatggcaga catcggcgcc
     1861 gatgagcccg gggctggtga gcccgacctg ggcgttcata ttggcgccgt ccatatagac
     1921 gagaccgccg ttctcatgga tcgtgcggca gatctccttt atgctttctt caaacacccc
     1981 atgggtggaa ggataggtga ccatcagacc ggccaggctg tccttgtatt gtatggcttt
     2041 ttccttcagg tcttccacat cgatatggcc ttcatcatcg ctcttgacga ctactacctt
     2101 catgcccgcc atgatcgcgc tggcagggtt ggtgccgtgc gccgaaatgg ggatcagcat
     2161 gacatcccgg tgggacccgt tgtgggcctt gtgataggcg cgaatcgtca gcagcccggc
     2221 atattcgccg ttggcgccgc tgttcggttg cagcgaagtg gccgcaaaac cggtgatctc
     2281 gctgagccat tcctcgagtt cggtaagaat gcggcgatat cccttccatt gatcggcagg
     2341 ggcaaaagga tgcaacccgc cgaattccgg ccagctgaca gggatcagct ccgtagcggc
     2401 gttgagcttc atcgtgcagg agccgagcga gatcatggaa gtgttcaggg agaggtcctt
     2461 gttctccagg ctccgcagat aacgcatcat cttcgtctcg gaatgatggc tgttaaagac
     2521 ggggtgggtg aggaactccg ttacccgggt gacggaggtg gggatattgg caagggtgtt
     2581 gtctacatcg aaggtggccg agacgagtga ggtcccggtg aggtgtccga acaccgagag
     2641 gatattgacc acatcttcct gggcggtcgt ttcatcgagg gtgatgccga ccgttccccc
     2701 gccggaataa aagaaattca ttccatgcgt ttccgccgcc tgccggaggg cgacagggtc
     2761 ttttactttt acccgcacgg tgtcgaaata atattcattc agattgggat accccatggc
     2821 ctccagttcg gtggcgaggg tccgggcgag caggctcacg cgtttggcga tgcgggttag
     2881 gccttccgcc ccgtggtaga cggcatacat cgccgacata ttggccagca gcgcctgggc
     2941 cgtacagata ttggaggtgg ctttttcgcg tttgatatgc tgttcgcggg tctggagcgc
     3001 catccgcaac gcgcggttac cctgggcatc gacgctgacg ccgatgatcc ggccggggat
     3061 cgaccgcttg aattcatcct tcgcggtgaa gaaggcggca tgcgggccgc caaagcccat
     3121 cggtacgcca aaacgctgcg cggagcctac ggccacgtcc gcgcccaatt ctcccggcgg
     3181 cgtgagcagg gtcaacgcca gcagatcggt tgccatcacg acataggcgc cggcgttatg
     3241 cacctgctgg ataaaggcgc tatgatcatt caccgatccg tcgctggcgg ggtattgtac
     3301 gatagccccg aagtacgagc catcgataac ggctttacga taatccccga ccaccagttc
     3361 gatcccgatc ggtaaagcgc gggtgagcag caggtccttt gtctgggcga agatattgcc
     3421 gtccacaaag aagcgggggg tagtgatatg atcatgatcc ttgttcttca ggttgaagaa
     3481 catcgtcatg gcctcggccg ctgccgtccc ctcatcgagc agggaggcgt tggccagggg
     3541 caggccggtc aggtcggcga
//
LOCUS       DATGFG010000457         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1264225, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000457 DATGFG010000000
VERSION     DATGFG010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264225"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..910)
                     /locus_tag="VKQ52_08470"
     CDS             complement(143..910)
                     /locus_tag="VKQ52_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87260.1"
                     /translation="MRFTIYGMLLGCLCPILLAAQDNYEIQVYGSQTVAKGNTMAELH
                     SNFTFDGSKTVESKVLPTNHVFHETIEITHGWNSWFETGFYFFNTLGNDGRTNYVGSH
                     IRPRVMAPAAWKWPVGVSLSMEAGYQKREYSEDDWTLEIRPIVDKQWGSLYVSFNPTF
                     DKSFHGLNKDLGYVFSPNLKAAYNITKIVAGGFEYYGSVGPLNHFQPAQSQQHQLFAA
                     LDLDWSPDWEFNIGYGWGFTQSTDNAIFKMILGYRFH"
     gene            complement(1061..>2273)
                     /locus_tag="VKQ52_08475"
     CDS             complement(1061..>2273)
                     /locus_tag="VKQ52_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ87261.1"
                     /translation="LLEAGVQPATLQLIYKNSLNTLPQEEVAVFDTAFHLLYHDAGDI
                     DKVKETQGMIDSIITFREIHFYYEDLQVTGFVYAFRGKTYVITAAARDDDGLAKLKAL
                     RLELGLGFAVAILLTLLAGVFFSRKALEPVSTMVEKVADISASHLDLRLSEGNGKDEI
                     AELALTFNRMLDRLEQSFEAQKQFVSNISHELRTPLAAIIAEAEVSAIKSRSPDEYRE
                     TLRLVLQDARKLSRLSGDLLDLAKASYDQAGIALKELRLDEVLLDARHVILQANPGYR
                     VDLIFEKEIGEEDSISIVGNEYLLRVAFSNLIENACKFSANKQCRITICFAEGNPVLR
                     FQDSGIGIARHDLQKIFTPFYRGENKRYAPGNGIGLSLTQRIITMHRGSIAVDSRQGE
                     GTTFTILLSRA"
BASE COUNT          512 a          680 c          578 g          503 t
ORIGIN      
        1 ccggttcttc tggtattctc ttacaggtgc acaggccggc aggataaccg ggaaaagggc
       61 cacgccgata acgaaaatga tggtttttct catatgtact tttttgtcgg aacacccgta
      121 tctgcctccc gggcctcctg gcttaatgaa accggtaccc caatatcatc ttgaagatcg
      181 cgttgtccgt gctctgggtg aatccccatc cataaccgat gttgaactcc cagtccggcg
      241 accagtcgag gtccagtgcc gcgaacaatt ggtgctgctg gctctgagca ggctgaaaat
      301 gattcaacgg cccaacggac ccgtaatact cgaacccacc cgccacgatc ttcgtaatat
      361 tgtaggcggc cttcaggttc ggcgagaaca catacccaag atccttgttc agcccgtgaa
      421 aggatttgtc gaaggtcggg ttgaaggata catacaggga gccccattgc ttgtccacga
      481 tcggccggat ctccagcgtc cagtcatcct cgctgtattc ccgcttctga taccccgcct
      541 ccatcgaaag actgaccccc accggccatt tccaggcagc aggcgccatc acccgcggcc
      601 ggatatgact ccctacatag ttggtgcggc cgtcgttacc aagtgtgttg aagaaataga
      661 acccggtctc gaaccaggaa ttccagccat gcgtgatctc gatcgtctcg tgaaagacat
      721 gattggtagg cagcaccttg ctctctaccg tcttcgatcc gtcaaacgta aaattgctgt
      781 gcagttccgc catcgtattc ccctttgcca ccgtttgact tccataaacc tgtatctcat
      841 aattgtcctg tgctgccaaa agaatgggac aaaggcatcc cagcagcatg ccatatattg
      901 tgaacctcat gttggagtaa aattaaagta gcccggtaaa gcagccggtt agagaccgat
      961 tataaataaa ttagaaaaaa attagaaaat tctgtagaaa atcgggatgc ctccaagggc
     1021 tcgcgggatg catcttatcc caggcctcgc cggctatatc tcaggccctg gaaagcagta
     1081 tggtaaaggt cgtaccctcc ccctgccggc tgtccacggc gatcgagccc cggtgcatgg
     1141 tgatgatgcg ttgggtcagg gaaagcccga tcccgttgcc cggagcatac cgtttgttct
     1201 cgccccggta aaaaggagta aagattttct gcaggtcatg ccgggcgatc ccgatgccgc
     1261 tatcctggaa acgaaggaca gggttccctt cggcaaagca gatggtgatg cggcactgct
     1321 tattggccga gaacttgcag gcgttctcga taagattgga gaaggcgacc cgtagcaggt
     1381 attcgttccc aacgatcgag atgctatcct cctccccgat ctccttctca aagatgagat
     1441 ccacccggta accgggattg gcctgcagga tcacgtgccg ggcatcgagc aatacttcgt
     1501 cgagccgcaa ctccttcagc gcgatcccgg cctggtcata acttgccttc gccagatcga
     1561 gcagatcgcc tgaaagccgg gacagcttcc ttgcatcctg caacaccagc cgcagggtct
     1621 cccggtattc atccgggcta cggcttttga tggcggacac ctccgcttcc gcgataatgg
     1681 cggccagcgg cgtccgcagc tcatgggaga tattgctgac gaactgtttc tgtgcctcga
     1741 aggattgctc cagccggtcg agcatccggt tgaaggtcag cgccagttcg gcgatctcat
     1801 ccttgccatt cccctcgctc agccgcagat cgagatgcga agccgagata tccgccacct
     1861 tctccaccat cgtggacaca ggctccagcg ccttccgtga aaaaaagacg ccggcgagca
     1921 gggtcagcag gatggcaacg gcaaagccca gccccagctc cagccgcagg gccttcagtt
     1981 tcgccaggcc atcgtcgtcc ctggccgcgg ccgttatcac ataggtctta ccccggaatg
     2041 catatacgaa cccggtgacc tgcaggtcct cgtaataaaa atggatctcc ctgaacgtga
     2101 tgatgctgtc gatcatcccc tgcgtctcct tcaccttgtc gatatccccc gcatcatgat
     2161 acagcagatg aaaagcggta tcgaaaaccg ccacctcttc ctgcggcagc gtgttcaacg
     2221 aattcttgta gatcagttgc agcgttgcgg gctgaacccc cgcctccagc agc
//
LOCUS       DATGFG010000458         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_373942, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000458 DATGFG010000000
VERSION     DATGFG010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373942"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VKQ52_08480"
     CDS             <1..411
                     /locus_tag="VKQ52_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HLZ87262.1"
                     /translation="AKEFGSQCVVTAIDTRMEADGQWYVYLNGGRVKTETLCMDWARE
                     AVDRGAGEILLTSMNNDGTKKGFALEITRRLAESLPVPVIASGGGGTPDHFVEVLGGG
                     KADAALAASIFHFKEITIPELKGYLQERGVAVRR"
     gene            445..1056
                     /gene="hisIE"
                     /locus_tag="VKQ52_08485"
     CDS             445..1056
                     /gene="hisIE"
                     /locus_tag="VKQ52_08485"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HLZ87263.1"
                     /translation="MTNVLIVNFMKSPDGLVPAIVQDHVTEKVLMLGYMNQEALDQTQ
                     KDGKVTFFSRSKQRLWTKGEESGNFLLVKQIAVDCDQDTLLIKAEPLGPVCHTGADTC
                     WDEKNHREDFLVCLEEIIEQRRLEGRPDDSYVARLFGKGINKIAQKVGEEAVELVIES
                     KDHDDQLFLNEVADLLFHYLILLNAKGFTLQDVTNVLRGRHKN"
     gene            1064..2158
                     /locus_tag="VKQ52_08490"
     CDS             1064..2158
                     /locus_tag="VKQ52_08490"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLZ87264.1"
                     /translation="MKLIFGVMLALPMMGLAQQAAPGFMITGKVVGLAENSAVFVTDA
                     SNPTDTVASSTVKGGQFALSGHVSEPNLFELNFASAKKKVPVFIGNDKMTLSGSVEDI
                     TKVKTTGSPSNDDFVAFQAMFTPYFTRLNAVMGQANSPAGASMKDSLFRVYKRLTDSI
                     FVSVDQFIAQRKNSYVSPFVLVVVNQLTEDVLAQQRRLQSLSAVVQKGYYAQYLAPQL
                     DKASVGAIGTSALDFTQNDTAGHAVSLSSFKGKYVLVDFWASWCGPCRMENPNVVATY
                     KKFKDKNFTVLGVSLDKAKEPWIKAIKDDGLAWTQVSDLKFWYNDAATKYHIESIPQN
                     LLVDPNGKIVARNLRGVELQQKLCELLGCN"
     gene            complement(2194..3312)
                     /locus_tag="VKQ52_08495"
     CDS             complement(2194..3312)
                     /locus_tag="VKQ52_08495"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HLZ87265.1"
                     /translation="MTKAKKITGLLAFLCLATAIPSSAQLTTLPDGGNKKASVSERIG
                     LTDVTINYDRPAVKGREGQIWGKLVAEGYTDQGFGTSKSAPWRAGANENTTITFSKDV
                     MVEGKPLPAGKYGFFIAYGANECTLIFSHNNSSWGSFFYDPKEDALRVTVKPVPMDKA
                     VERLRYEFVDEKENAATIALEWEKLSIPFTVETDYVNQQLESFRRELRDSKGFTWNSY
                     MQAAQFCAQRNTDLQEGLAWADQSISLPFIGDKNFQTLSTKAQILSELNRKDEADAVM
                     KEALPLGKMQDVHQYARQLLTQKRAKEAFDAFQFNYTKHPNEFTTNMGMARGYSGMGD
                     YKKAIEYLKKAQAQAPDNVNKNNIAQLMPKLEKGQDIN"
     gene            complement(3584..>4248)
                     /locus_tag="VKQ52_08500"
     CDS             complement(3584..>4248)
                     /locus_tag="VKQ52_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ87266.1"
                     /translation="GDVWSTNAAQNQVVQCFTQELTVSANIGQVNNLCVVSLPVTLLS
                     FSGRETGNSTVLLSWQTAAEANNKGFAVERKTGAGDFQQVGFVTSQEPDGNSNATLDY
                     QYGENLPSGGEFQYRLAQEDRDGKKQYSAVISIHSDGSAAGKLLVYPNPAPGGHMTVT
                     FDSPDKRDLTISDASGRVIRVIRNITDNTCQISNLRTGIYFLKVTSNAGGGSRLAKLI
                     VQ"
BASE COUNT          873 a          965 c         1363 g         1047 t
ORIGIN      
        1 gcgaaggagt ttggttccca gtgtgtggtg acggctatcg atacgcggat ggaggcggat
       61 ggacagtggt atgtgtatct gaacggcgga agggtgaaga cggagaccct gtgtatggat
      121 tgggcccgtg aggcggtgga ccggggggcg ggggagatcc tgctgacctc gatgaacaac
      181 gacgggacga agaagggttt tgcgctggag atcacccggc gactggccga atcgctgccg
      241 gtgccggtga ttgcctccgg cggcggcggg acgcccgatc attttgttga ggtgctgggg
      301 ggcgggaagg ccgatgcggc gctggcggcc agtatcttcc attttaagga gatcacgata
      361 ccggaattaa aaggatattt gcaggagcgc ggagttgccg tgcgcaggtg atcgacgact
      421 tgcctgagaa taaaaactga tcgtatgaca aatgttttaa tcgtcaattt tatgaaatcg
      481 cccgatggcc tggtacccgc tattgtccag gaccacgtca cggagaaagt gctgatgctg
      541 gggtatatga accaggaggc gctggaccag acacagaaag atggaaaggt tacttttttc
      601 agccggagca aacagcggct ttggacaaag ggcgaggaga gcgggaattt cctgctggtc
      661 aagcagatcg ccgtggattg tgaccaggac acgctgttga tcaaggcgga gccgcttggc
      721 ccggtttgtc ataccggcgc cgatacctgc tgggacgaaa agaaccaccg ggaagatttt
      781 cttgtctgcc tggaagagat catcgaacag cggcgtcttg aggggcgtcc ggatgattcc
      841 tatgtcgcca ggctttttgg caaggggatc aacaagatcg cgcaaaaggt tggggaagag
      901 gcggtagagc tggtgatcga atccaaggat catgatgacc agctttttct caacgaggtg
      961 gcggaccttt tgttccatta tttaatactc ctcaatgcaa aaggatttac cttacaggat
     1021 gtaaccaatg tgttgcgggg aagacataaa aattaaagaa tatatgaagt tgatttttgg
     1081 ggtaatgctg gcattgccga tgatgggttt ggcgcagcag gcggcgcccg ggtttatgat
     1141 cacggggaag gtggtagggc tggcggagaa ttccgcggtc tttgtcacgg atgcttcgaa
     1201 cccgacggat acggtggcgt cctcgaccgt aaagggtggg cagttcgcgt tgtccggtca
     1261 tgtatcggag ccgaatcttt tcgaactgaa ttttgcgtcg gcgaagaaga aggttccggt
     1321 ctttatcggg aatgataaaa tgaccttgag cggatcggtg gaagacataa cgaaggtgaa
     1381 gacgacagga tcaccgagca atgatgattt cgtggcgttc caggctatgt tcacgcccta
     1441 tttcacccgg ctgaatgccg tgatgggcca ggctaattcc cccgcgggcg catcgatgaa
     1501 ggattcgctt ttccgggtgt ataaacgcct gaccgattcc atttttgtat ccgtggatca
     1561 atttatcgct cagcgaaaaa actcttatgt ctcgcccttc gtgctggtag tcgtgaacca
     1621 gcttacggaa gatgtgctgg cgcagcaacg gcggttgcaa tcgctttcgg ccgttgtgca
     1681 gaaagggtat tatgcgcagt acctggcacc gcaactggac aaggccagtg tgggggcgat
     1741 agggacgagt gcgcttgatt ttacgcagaa cgatacggcg gggcatgctg tttccctttc
     1801 ttccttcaag gggaaatacg tgcttgtcga tttctgggcc agctggtgcg ggccttgccg
     1861 gatggaaaat ccgaatgtgg tagcgacgta taagaaattc aaggacaaga attttaccgt
     1921 gcttggggtc agcctagaca aggcgaagga gccctggatc aaggcgatca aggatgacgg
     1981 gcttgcctgg acgcaggtga gtgatctgaa gttctggtat aatgatgcgg ccaccaaata
     2041 ccatattgaa agcattccgc agaacctgct ggtggaccct aatgggaaga tcgtggcgcg
     2101 gaacctgcga ggggtggaat tgcagcagaa gctctgcgag ctgctggggt gtaattaaga
     2161 gaaaaccctg cctgacggca gggtttttta ctatcagttg atgtcctgtc ctttctccag
     2221 tttcggcatc agttgcgcga tgttgttctt gtttacatta tcgggggcct gtgcctgcgc
     2281 tttcttcagg tattcgatgg cttttttgta gtcgcccatg cccgagtatc ctcttgccat
     2341 gcccatattc gtcgtgaatt cgttgggatg ttttgtgtaa ttgaactgga aggcgtcgaa
     2401 ggcctccttt gctcttttct gtgtcaggag ctgacgggcg tattgatgca cgtcctgcat
     2461 ctttccgagg gggagggctt ccttcatgac ggcgtcggct tcgtccttgc ggtttagctc
     2521 gctgaggatc tgcgccttcg tggagagggt ctggaagttc ttgtcgccga tgaaggggag
     2581 gctgatggac tggtcggccc aggcgagtcc ttcctgcaga tcggtgttgc gctgtgcgca
     2641 gaactgggcg gcctgcatat aggaattcca ggtgaagcct ttactgtcgc ggagttcgcg
     2701 gcggaagctc tccagttgct gattgacata gtcggtttcg acggtgaagg ggatgctgag
     2761 cttttcccat tcgagggcga tggtggcggc attctctttc tcgtcgacga actcgtatct
     2821 caatctttcc actgctttat ccatcggcac gggttttacc gtcacgcgga gggcatcttc
     2881 ctttggatcg tagaagaagc tgccccagga ggagttgttg tgagagaaga taagggtgca
     2941 ttcgttggcc ccgtaggcga tgaagaaccc gtatttccct gccggcaggg gtttgccttc
     3001 caccatgacg tccttgctga aggtgatggt cgtgttctcg tttgcgcctg cccgccaggg
     3061 ggctgatttg ctggtgccga agccctggtc ggtatagcct tcggcgacca gttttcccca
     3121 gatctgtcct tcgcggcctt tgacggcggg gcgatcgtag ttgatggtaa catcggtgag
     3181 gcctatgcgc tcgctgacgg aggccttttt atttccgccg tcggggaggg ttgtcagctg
     3241 ggctgaggat gggatggcag tggcaagaca tagaaaggcc agcaggccgg tgattttttt
     3301 ggctttggtc atatggatgc ttttagctga tgaataatgc ggacagttag cggatcaaaa
     3361 atagggtatg tgtttatcgg gtgaaaggga atttacaccg aaggcggata gcagggatat
     3421 ggagcggcgg ccgctgccgc gttggccgag gcgttgaaaa acggaggcca atcgttgaag
     3481 attggcctct ttgccgctgg gatcatcggg ctggggtagt ccccgggccg aagaacggga
     3541 ccgaagaaat ggaaccgggg gggcgccgga tcgccggcga gtcttattgc acgatcagtt
     3601 tggccaggcg gctcccgccg ccggcgttgg atgtaacctt cagaaaatag atgcccgttc
     3661 ttagattact gatctgacag gtattgtctg ttatatttcg gattaccctt atgaccctgc
     3721 cgcttgcgtc gctgatggta agatctcttt tgtcagggct gtcgaaggtg actgtcatgt
     3781 gaccacccgg cgcggggttg ggatatacca ggagtttgcc ggcagcggac ccgtcggaat
     3841 ggatggagat gaccgcgctg tattgttttt tcccatcgcg gtcttcctgg gcgaggcggt
     3901 attggaactc cccgccggaa gggagatttt caccatactg gtaatcgagc gtggcgttgc
     3961 tgttgccatc cggctcctgc gaggtgacaa aacccacctg ctgaaaatca cctgcgccgg
     4021 tcttccgttc gaccgcgaag cctttattat tggcttcggc agccgtttgc caggatagta
     4081 atacagtaga gttgcccgtt tcgcggccac tgaaggagag gagcgtgacg ggcagggaaa
     4141 caacgcatag attgttgacc tggccgatgt tggcggagac ggtgagttcc tgtgtgaagc
     4201 attgtaccac ctggttctgg gctgcattgg tcgaccagac atctcctt
//
LOCUS       DATGFG010000459         9136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_973936, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000459 DATGFG010000000
VERSION     DATGFG010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973936"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="VKQ52_08505"
     CDS             complement(<1..1657)
                     /locus_tag="VKQ52_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HLZ87267.1"
                     /translation="MHRFLIALLLLTAAIDSRAQSNLATQANYQAAARFSPKKLEKMI
                     FTTQVDAHWLKKSTRFWYMYETTEGKKWYIVDPLKSEKKTLFDNDRLAAAISRIVKDP
                     FEAKHLGLDSLRFIKDENWIQFEVKSSESLRKKDSSKDSTAKKIYYFEYNLLDGSLNE
                     LTDYKKPKHKPAWAGISPDSNTIVFGRHFNLFWMDRANYEKALKNEDDSTIVEHALTT
                     DGVENYSYHGNGVLGGGGETNVDKEKNKDKRKPAQLFWSPDSKHFAMSRSDMRKVKDL
                     WVINALADPRPTLETYKYMMPGEKEAPIEQLLVFDLTEHTHQVISAAEFRDQDLAIWS
                     APTQERRRDDDYRTQLWLGPSSKFYFTRTSRDLHRIDACSVTIGDTIAQPLIKERLNT
                     YVEVRRLGLVSEGRELIEWSERDGWAHFYLYDGTGKQKNQITSGPFHCEDIVGIDDKK
                     RILYFTASGREAGENPYYLHLYKVNLDGSGLTLLNTGDFDHGVSMDDDQQFFVDNFSR
                     VNTIPRSTLYTSSGRKIMELQTADLSALLAAGYKFPEPFTVKAD"
     gene            1811..2566
                     /locus_tag="VKQ52_08510"
     CDS             1811..2566
                     /locus_tag="VKQ52_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HLZ87268.1"
                     /translation="MFRMLPYIPGYFYYISIVLQAICVVHCIRRGTQQKWIWLIVFLP
                     YIGCIIYLFMEILPRRRMGDWQAGLGSLIVSPAARIRRLEENLQFADTFNNRVLLANG
                     YMLTGRTEEAIELYSSSLTGAFSENEYVINRLIAAYFQTGRYPELIQLARKIYTTAPF
                     ARSEAHLYYARALDSTGDKEGAEREFRKMKGRFADFEARYQYGLFLQREGRGGEAAGI
                     LEDIVREGPHLSSRERRANYLWIQKSREELKKG"
     gene            complement(2580..2948)
                     /locus_tag="VKQ52_08515"
     CDS             complement(2580..2948)
                     /locus_tag="VKQ52_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HLZ87269.1"
                     /translation="MRYSDAELGEFRELINRKLDAAKKELAYLQGLITRKDEMGGDND
                     DARYMTMEDGSMSMEREQLSQMASRQITFIDHLEKALMRIENKTYGICRVTGKLIDKA
                     RLRAVPHATLSIEAKQMMNK"
     gene            3265..3531
                     /locus_tag="VKQ52_08520"
     CDS             3265..3531
                     /locus_tag="VKQ52_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87270.1"
                     /translation="MDTATFFVGRGADFFAGAAGATFLAAGLAILLAAGAFFTGLLPP
                     DEWADFDAVFLAGAIFLTFTVFLAGFAAFFLVAIQLGFFSDEHF"
     gene            complement(3502..7005)
                     /gene="ileS"
                     /locus_tag="VKQ52_08525"
     CDS             complement(3502..7005)
                     /gene="ileS"
                     /locus_tag="VKQ52_08525"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HLZ87271.1"
                     /translation="MANKYKEYQQLNLPSISQEVLAKWESTRAFEKSVELREGNTPFV
                     FYEGPPSANGLPGIHHVISRTLKDLVCRYKTMKGFQVKRKGGWDTHGLPVELGVEKML
                     GITKEDIGKKISIAEYNETCRREVLKYKDKWDELTRRMGYWVDLKDPYITFENNYIET
                     LWWILAQLYNKGLLYESVSIQPYSPAAGTGLSSHELNQPGCYKDVKDTSVVAMFKAVR
                     NPKSEFLFKLAGQAGQTGQDAADAEVFFMAWTTTPWTLPSNLGLTVGPRIDYVLVKTF
                     NPYTHLPIHVVLAKALINKYFKAEGENGGFENVSPDAKILPWKILGEFKGATLEELRY
                     EQLLPYEANSPEKAGGDPFRVLLGDFVTTEDGTGIVHTAPAFGADDYRVGMKYNIGIL
                     TMVDKQGKFLEGLGEFSNRYVKNYKDDPNYADVNVDICVMLKKENRAFRIEKYEHNYP
                     HCWRTDKPVLYYPLDAWFIRTTALKDRMVELNKTINWKPKSTGEGRFGNWLENMVDWN
                     LSRSRYWGTPLPVWRTELPENPTDQDLANRKAVCIGSIAELQTALKASIKAGFMKPAK
                     GDTEEAYIQKLTADLHKPYVDEIILASEDGRPMYRVADLIDVWFDSGAMPYAQWHFPF
                     ENKEVFARNYPADFIAEGVDQTRGWFYTLHALGSLLKESIHEELKDISPSVLKPGSQG
                     PVTAAGLDARYPGLAYKTVVSNGLVLDKNGNKMSKRLGNVVDPFDTIGKFGADATRWY
                     LITNASPWESMKFDLDGIKEVQRKFFGTLYNTYQFFALYANVDGFTFKEAYVPPAERP
                     EIDRWILSSLNSLVTKVIDGLDDYEPTLAGRLIEDFVDEHLSNWYVRLCRRRFWKGEY
                     EHDKICAYQTLYECLETLSRLMAPISPFFADWLFVNLNEVTGRIKADSVHHADFPAAN
                     ADFIDLLLEERMQLAQDASSLILSLRKKVNIKVRQPLQKVLVPVLNPGMKDQLEKVED
                     LIKAEVNIKEIQYLGGTDGFIKKKIKPNYIALGKKLGPKMKAVSAALTQFSQEEIAKL
                     EKEGDITLLIDKEPIILSIQEVDISSEDIPGWVVANKNALTVALDVTVTPQLAQEGNA
                     REFVNRIQKIRKDNGYDLTDRILVKVADVPSLTDSLTQFNDYICAEILADSIELVKEP
                     ANGTEIEVNDVLLKVFVTKKA"
     gene            complement(7722..8249)
                     /locus_tag="VKQ52_08530"
     CDS             complement(7722..8249)
                     /locus_tag="VKQ52_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HLZ87272.1"
                     /translation="MYNYVKALHVIFIVTWFSGLFYMVRLFVYHTEANAKPEPERTIL
                     LQQFRLMIRRLWLGITWPSAILTLIFGPWMMGLMGSIPAWLMIKMGFVVGLYLYHFSL
                     QVIYRQQMKGIFRYSSQQLRIWNEVATIFLVAIVMLAVVKQYLSWAWGLIGLFLFVVL
                     LMTAIKIYKKIRARS"
     gene            complement(8299..>9136)
                     /locus_tag="VKQ52_08535"
     CDS             complement(8299..>9136)
                     /locus_tag="VKQ52_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HLZ87273.1"
                     /translation="EGSPLIVLTRQLQAALQQLVDEPVGIAMRYGNPSMEDAYEDLLK
                     KTPQLEEVIAIPLYPHYAMASYETAVEYAQEIHRRKKYSFRLNFIKPFYNEKHYLNAL
                     SEHIRPYLKQGYDHILFSYHGIPGRHIRKSDPTGNHCLQSAACCETASPAHATCYRHQ
                     CFITTREIVRLLDIPEGKYSNSFQSRLGKGWLEPFTDIRLEEMPKEGIKKLLILCPAF
                     VSDCLETLEEIAMRGKETFLGAGGGEYTMIPCLNIDPLWVDALAGWVRDYAAGDRTMI
                     LD"
BASE COUNT         1736 a         2082 c         2840 g         2478 t
ORIGIN      
        1 catcggcctt tacggtaaag ggctctggga atttatagcc ggcggccagg agggcgctga
       61 ggtcggcggt ctggagctcc atgatcttcc ggccgctgct ggtatagagg gtggatcgtg
      121 gtatggtatt gacgcgggag aagttgtcga cgaagaattg ctgatcgtcg tccatgctga
      181 cgccgtgatc gaaatcgccg gtgttcagca gggtgaggcc gctaccgtcg aggttgacct
      241 tatacaggtg cagatagtag ggattttcgc cggcttccct gccgctggcg gtaaaataga
      301 ggatgcgttt tttgtcgtcg atgccgacga tgtcctcgca atggaagggg ccgctggtga
      361 tctggttctt ttgttttccc gtgccgtcgt agaggtagaa atgggcccag ccgtcgcgtt
      421 cgctccactc gattagttcc cggccctcgc tcacgaggcc gaggcggcgt acttccacat
      481 aggtgttgag gcgttctttt atcaggggct gggcgatggt gtcgccgatg gtgacggagc
      541 aggcgtcgat gcgatggagg tcgcggctgg tgcgggtgaa atagaatttc gaggaggggc
      601 cgagccagag ctgggtgcga taatcgtcgt cgcggcggcg ttcctgggtg ggcgccgacc
      661 agatggcgag gtcctggtcc ctgaattcgg ccgcgctgat gacctggtgg gtatgttcgg
      721 tcagatcgaa gaccaagagt tgttcgatgg gcgcttcttt ttcgccgggc atcatgtatt
      781 tataggtctc gagtgtgggg cggggatcgg caagagcgtt gatgacccag aggtctttta
      841 cttttcgcat atccgagcgt gacatggcga agtgtttcga gtcggggctc cagaagagct
      901 gggccggttt acgtttgtcc ttgttctttt ccttgtcgac gtttgtctcg ccgccgccgc
      961 cgaggacgcc attgccatga tagctgtagt tttccacgcc gtcggtggtg agggcgtgct
     1021 cgacgatggt gctgtcgtcc tcgttcttca gcgctttttc gtagttggcg cggtccatcc
     1081 agaagagatt gaaatggcgg ccgaagacga tggtgttgct atcgggggag atgccggccc
     1141 aggcgggttt gtgttttggt tttttatagt cggtgagttc gttgagggag ccgtcgagga
     1201 ggttgtattc gaagtaatag atctttttgg ccgtggaatc tttggaggag tcttttttgc
     1261 gcaacgactc ggagcttttt acttcgaact ggatccagtt ctcgtctttt atgaagcgaa
     1321 ggctgtcgag tccgaggtgt tttgcttcga aggggtcttt tacgatgcgg gagatggcgg
     1381 cggccagtct atcgttgtcg aacagggttt ttttctcgct tttgaggggg tcgacgatat
     1441 accatttctt tccttcggtg gtttcataca tataccagaa gcgggtgctt ttctttagcc
     1501 agtgagcgtc cacttgtgta gtgaagatca ttttctccag cttttttgga gagaagcggg
     1561 cggcggcctg gtaattggct tgggttgcga ggtttgactg ggcccgggag tcaatggctg
     1621 ccgtgagcag cagcagggct atcaggaagc ggtgcataag cagttattcg tttaattatg
     1681 ctatataata ttttcttttc cagccatttc ggttatatcc atccgcggtg gcatttcagg
     1741 atgagtgggt ggaaaaactt ttattatttt aggctcaaac tcccgaattt taaacccaaa
     1801 ctcccgaccc atgtttcgca tgctccccta cattcccggt tatttttact atatctcgat
     1861 cgtgttgcag gcgatctgtg ttgtgcattg tatccgcagg ggtacgcagc agaaatggat
     1921 ctggctgatc gttttccttc cttatatcgg gtgtatcatc tacctgttca tggagatcct
     1981 gccgcgccgg aggatgggcg actggcaggc tggtcttggt tctttgatcg tgagtcccgc
     2041 ggcgcggatc aggcggctgg aggagaacct gcagtttgcc gataccttca acaaccgggt
     2101 gttgctggcc aatggctata tgctgacagg gcggacggag gaggcgatag aactttactc
     2161 ttcgagtctt acgggcgcct tttcggagaa cgagtatgtg atcaaccggc tgatagcggc
     2221 ttattttcag acggggcgtt acccggagct gatccagctt gcgaggaaga tctataccac
     2281 ggcgcctttt gcccggagtg aggcgcatct atattatgcg cgggcgttgg atagtacggg
     2341 tgataaggag ggtgcggagc gggagttcag gaaaatgaag ggccggtttg cggatttcga
     2401 ggcccggtat caatatgggc ttttcctgca gcgggagggg cgtgggggtg aggccgcggg
     2461 tatcctggag gacatagtaa gggagggccc tcaccttagt tccagggaga ggcgggccaa
     2521 ttatctctgg atacaaaagt caagagaaga actaaagaag ggttgacccg tggggcgcct
     2581 tatttattca tcatttgttt tgcctcgatg ctcagggtgg cgtggggaac ggctctgagc
     2641 ctggctttat cgatcaattt tccggtgacc cggcaaatac cataggtctt gttctcgatc
     2701 ctcatcaggg ctttctccag atggtcgata aaggtgatct ggcggctggc catctggctc
     2761 agctgctccc tttccatgct catgcttcca tcttccatgg tcatgtagcg ggcgtcatca
     2821 ttgtcgccgc ccatttcgtc cttacgtgtg atcaatccct ggaggtaggc caattccttt
     2881 tttgctgcgt ccagtttgcg gttgatcaat tccctgaatt ctccaagttc tgcgtcgctg
     2941 tatctcatag tgggtccagt ttgttcttct tgtggggtgt ctaatactga ttttgtgaat
     3001 tccggttcgt atttgcgaac cgtttttgtt tttatttccg ggatgaccac ctttaccggt
     3061 ttagcgggga tagccggttt ttttgcttcc ggtctcggcg ctacgggcgg gggtggtggg
     3121 ggtgcggaga caaccgattt ggcaacaacg ggtgtagcgg ccggtttaac ggtcgcctgg
     3181 ggttgttggg cttttgcggc cgggatggtt gccttagagg taggggttgc agcggggtga
     3241 ctctttgctg cgggcttaga agtattggat acagctactt tttttgttgg taggggggcg
     3301 gatttttttg ccggtgctgc gggggcaacc tttttggcgg ccggtttggc aatactctta
     3361 gcagccgggg ctttctttac gggcttgctg ccacctgacg agtgggcaga ttttgacgcc
     3421 gtttttttag ccggtgctat tttcttgaca ttcacggtct ttttagcggg ctttgccgct
     3481 ttcttcttgg ttgccataca gttaggcttt tttagtgacg aacactttta aaagaacatc
     3541 attcacttca atttcagtgc cattcgcggg ttccttcacc aattcaatgg aatctgccaa
     3601 aatttccgcg caaatataat cattaaattg ggtcaaggaa tcagtaagag agggtacgtc
     3661 tgctacttta accaatatcc tgtcggtcag gtcataaccg ttgtctttcc ggattttttg
     3721 gatgcgattg acgaactcac gtgcattgcc ttcctgggcc agttgggggg ttacggtaac
     3781 atccagggcg acggtgagcg catttttgtt tgccactacc cagccgggga tatcctcact
     3841 ggaaatatcg acttcttgga tagacaatat tatgggttct ttatcaatta ataaagtaat
     3901 atcaccttct ttttccaatt ttgctatctc ttcctgcgaa aattgcgtga gcgcagcgct
     3961 gacagccttc attttaggtc ccagtttttt ccccagggcg atgtagttgg gttttatctt
     4021 cttttttata aagccgtcag taccaccgag atactggatc tccttgatat tgacctcggc
     4081 ctttatcagg tcttctactt tttcgagctg gtccttcatc ccggggttga ggacgggaac
     4141 cagcactttt tgcaggggtt ggcgaacctt gatattcact tttttccgca gcgacaggat
     4201 gagggaagag gcgtcctggg cgagctgcat gcgctcttcg agcagcaggt cgataaagtc
     4261 cgcgttggcg gcggggaagt cggcgtggtg tacggagtcc gcctttatgc ggccggtaac
     4321 ttcgttcagg ttgacgaaga gccagtcggc gaagaagggg gagatgggcg ccatcagcct
     4381 gctgagggtt tcgagacatt catagagggt ctggtaggcg cagatcttgt cgtgttcgta
     4441 ttcccctttc cagaagcggc ggcggcagag gcggacatac cagttgctga ggtgttcgtc
     4501 gacgaagtct tcgatgagcc ggccggcgag ggtgggctca tagtcgtcga gtccgtcgat
     4561 cactttggtt accaggctgt ttagagaaga caggatccaa cggtcgatct ccggtctttc
     4621 ggcgggcgga acataggctt ctttaaaggt gaagccgtcc acgttggcgt agagggcgaa
     4681 gaactggtag gtattgtaca gtgttccgaa gaatttccgt tggacctctt tgatcccgtc
     4741 gaggtcgaat ttcatgcttt cccaggggga ggcattggtg atgaggtacc agcgggtggc
     4801 gtcggcgcca aatttcccga tcgtgtcgaa gggatcgacg acgttgccga ggcgtttgga
     4861 catcttgttg ccgttcttat cgagcacgag gccgttggaa accactgtct tataggccag
     4921 cccggggtaa cgggcgtcga gcccggccgc ggtgacgggg ccctgggagc ccggtttgag
     4981 tacggagggc gaaatgtcct ttagttcttc gtggatggac tctttcagga gggagccgag
     5041 ggcgtggagg gtatagaacc agccgcgggt ttggtcgacg ccttccgcga tgaagtcggc
     5101 gggatagttc cgggcgaaga cctccttatt ttcgaagggg aagtgccatt gggcgtaggg
     5161 catggcgccg ctgtcgaacc agacgtcgat gaggtcggcg acgcggtaca tcgggcgacc
     5221 atcttcgctt gccaggatga tctcgtccac gtatggcttg tgcagatcgg cggtaagctt
     5281 ttggatatag gcttcttcgg tgtcgccttt tgcgggcttc atgaagcctg cttttatgga
     5341 ggctttcagg gctgtctgca gttcggcgat ggagccgatg caaaccgctt tgcgattggc
     5401 gagatcctgg tcggtggggt tttccggcag ttcggtgcgc cagacgggta agggggtacc
     5461 ccagtaccgg ctgcggctga ggttccagtc gaccatattt tccagccagt tgccgaagcg
     5521 gccttcgccg gtggattttg gtttccagtt gatggtcttg ttcaattcga ccatgcggtc
     5581 cttgagggcc gtggtgcgga tgaaccaggc gtcgaggggg taatagagga cgggtttgtc
     5641 ggtgcgccag cagtgcgggt agttgtgttc gtatttctcg atgcggaagg cgcgattttc
     5701 ttttttgagc atgacgcaga tgtccacgtt gacatcggcg tagttggggt cgtccttata
     5761 gttctttaca tagcggttgc tgaattcgcc gaggccctcc aggaatttcc cctgtttgtc
     5821 gaccatggtg aggataccta tattatattt catcccgacc cgatagtcat cggcgccgaa
     5881 ggcgggcgcg gtgtggacga tgccggtacc gtcttcggtg gtgacgaagt cgccgaggag
     5941 tacgcggaag ggatcgccgc cggctttctc ggggctgttg gcttcatagg gaaggagttg
     6001 ctcgtatcta agctcttcga gggtggcgcc tttgaattcg ccgaggatct tccaggggag
     6061 gatctttgcg tcgggggaga cgttctcgaa cccgccattc tcgccttcgg ctttgaaata
     6121 tttgtttatc aatgcctttg ccaggacgac gtggatggga aggtgtgtgt atgggttgaa
     6181 ggtctttacc agtacgtagt cgatacgcgg gccgacggtg agacccaggt tagaagggag
     6241 ggtccagggg gtggtggtcc aggccatgaa gaagacttcc gcgtccgcgg catcctgtcc
     6301 ggtctgcccg gcctgtcctg ccagtttgaa gaggaattcg cttttgggat tgcggacggc
     6361 tttgaacata gcgacgacgg aggtatcctt tacatccttg tagcagccgg gctggttgag
     6421 ctcgtgggag ctgaggccgg tgccggcggc gggggaatag ggctggatgc tgacgctttc
     6481 gtagaggagt cctttattat atagttgtgc gaggatccac cagagggtct cgatatagtt
     6541 gttctcgaag gtgatatagg ggtctttgag gtcgacccaa tagcccatgc ggcgggtgag
     6601 ttcgtcccat ttgtccttgt atttcaatac ttcgcgacgg catgtctcgt tgtattccgc
     6661 gatggagatc tttttgccga tatcttcctt ggtgatgccg agcatttttt cgacgccgag
     6721 ttcgacgggg aggccgtggg tatcccagcc gcctttgcgt ttgacctgga agcctttcat
     6781 ggtcttatag cggcagacga ggtctttgag ggtacggctg atgacgtggt ggatgccggg
     6841 gaggccgttg gcgctggggg ggccttcgta aaatacgaat ggggtattgc cttcccgtaa
     6901 ttccacactt ttttcaaagg ctcttgtgga ctcccatttc gccaggactt cctggctgat
     6961 ggagggtaag tttaattgtt ggtattcctt atacttgtta gccattgtgc tataatgcgt
     7021 taattgtgga tttgcgggtg agggcggccg gtagcggcca ttggggacgc gaatttacga
     7081 aaaaagggcc aataatcggg tgttttggca cggggggaag ggtggtcgca gggtaaggga
     7141 gtaattttgg cttgaggatt gtggtgggga aatggaggca ggccggaaaa tgttaaagga
     7201 ttgccaaaag gtcgctaaat cgatttaatt tgggggatgg gaggccggag ttatccacat
     7261 taacgggttt tggcataatt tctgaatact attttccaga aaagcgatac aaaacgggtc
     7321 atcacccgat caagatatac aagctattct aactctttct acggtttagg gtttaggggt
     7381 taaaaagggg ggtattccta ctccccttcc ttttaaaata ccctggtcgc ttatgcagtt
     7441 ggttcgtcag gacgaacatt agcaatccca ccgtatcgcc ccagttttcc ttattccgtg
     7501 tacattattt accttattca atggagatcc gtagccatcc cgtttttaat ctctgatatt
     7561 accttgcaaa aaacccgttc gacaagcacc aggctgtaag atctcaattc ctgaatcacc
     7621 agtgtacgaa tttcaattcc ggaaaaccta ccttttttac cccggaggac gtaaccctcc
     7681 cggtcttcga ccgggagggt tgtcattttt gggcagtgct ctcaggagcg tgcgcggatc
     7741 ttcttgtaaa tttttatggc ggtcatgagc aggacgacga agaggaagag gccgatgagg
     7801 ccccaggccc agctcaggta ttgtttgacg acggcgagca tgacgatggc gacgaggaag
     7861 atggtggcca cttcattcca gatgcgcagt tgttgcgagg agtagcggaa gatgcctttc
     7921 atctgttgtc tatagatgac ctgcagcgag aaatgataga gatagagccc gacgacgaaa
     7981 cccattttga tcatcagcca ggcagggatc gaacccatga ggcccatcat ccaggggccg
     8041 aagatcagcg tgaggatggc ggagggccag gtgataccga gccagaggcg gcggatcatc
     8101 aggcggaact gctgaagcag gatcgttcgt tcgggttcgg gtttcgcgtt ggcttcggtg
     8161 tgatagacga agagccgcac catataaaag agtccgctga accaggtgac gataaagatg
     8221 acgtgcagtg cctttacata gttatacata ggaaaaaatt tccaacatgg tgtgggggcg
     8281 tcccgacccg gggcgtcctt agtccaggat catcgtcctg tcgcccgccg cataatccct
     8341 gacccatccc gccaacgcgt ctacccacag cggatcgata ttgagacagg ggatcatcgt
     8401 atactccccg ccgcctgcgc cgaggaaggt ttcttttccc ctcatggcta tttcttccag
     8461 ggtctcgagg cagtcgctca caaatgcggg acagaggatg agcaatttct ttattccttc
     8521 ttttggcatc tcttccagcc ggatgtcggt gaagggttcg agccagcctt taccgagcct
     8581 tgactggaag gagttgctgt attttccttc ggggatatcg agcaggcgta ctatttcccg
     8641 ggtggtgata aagcattgat ggcgatagca ggtggcgtgt gccggcgagg ccgtctcgca
     8701 acaggcggcg gattgcaggc agtgattgcc cgtggggtcc gatttccgga tgtgccggcc
     8761 cggtatgcca tgataactaa acaggatatg atcatatccc tgtttgagat aggggcggat
     8821 atgttcgctc agggcattca gataatgttt ttcgttatag aacggcttga tgaaattcag
     8881 ccggaaggag tatttcttcc tgcggtggat ctcctgcgcg tattccaccg cggtctcgta
     8941 agaggccatg gcataatggg gatagagggg gatggcgatc acctcttcca gctgcggcgt
     9001 ctttttcagc aggtcttcat aggcatcttc catggatggg ttgccatagc gcatggcgat
     9061 cccgaccggt tcgtcgacca gctgttgcaa tgccgcctgg agttggcggg tcaggacgat
     9121 gagcggggag ccttcc
//
LOCUS       DATGFG010000460         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1561503, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000460 DATGFG010000000
VERSION     DATGFG010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561503"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..2106
                     /locus_tag="VKQ52_08540"
     CDS             262..2106
                     /locus_tag="VKQ52_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87274.1"
                     /translation="MKRHKTTPSGRTYFSFPALSIRQQLPLLICLLLLVLIALFGSIS
                     YLGVRRASMAVGEQRLQTLTEELSSLFQQSGHTLAVGTQALARQENIVNYFTGDSVNR
                     VASVKAMEVLQKMLADSQTIRAEVRDLQGRLLLQAGRGASSVHGLGSATEAKRQWAGP
                     DVSGEFGPGNRDSNFVGRLYLAGDSMFYPIVARVNRGERAEGWLLRWRPVRATQKSVA
                     QLSHLLGAKATLYFGNTDGSLWTDLLKQVTNTSPAVKELRKTLHYRAQGGKVLAYAMP
                     IAGTRWLILVELSEDLILEAANRFLYWMLGIGAVLVITGSLIAWLISRNVTRRLDRLM
                     WATTVIAEGDYSSQVPVDRQDEIGRLAASFNTMTMQVRHTQEDLEEKVKLRTHELETV
                     NKELEAFSYSVSHDLRAPLRAVSGYAMMLKEDYEHSFDDEARRITGNILANVKMMGRL
                     IDDLIGFSRLGKRDVRRQHTPMKDLVDVCVAELTPAWPEGKFQVNVGVLPACMGDSDL
                     LKQVWLNLVSNAMKYSSRREEPQIAIGAMEEAGGTVYFVRDNGAGFDMRYADKLFKVF
                     QRLHSQEEFEGTGVGLALVKRILEKHRGEIWAESSPGNGAAFYFRIPA"
     gene            2132..>2321
                     /locus_tag="VKQ52_08545"
     CDS             2132..>2321
                     /locus_tag="VKQ52_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87275.1"
                     /translation="MFNHVEVLLVEDNIHDAEMTIRALKKVNLANNLVHVKDGEEALD
                     FVYARGGFSNRQSADLPKV"
BASE COUNT          518 a          576 c          703 g          524 t
ORIGIN      
        1 cctgtttctg caaatagtcg tgaagctggt ccctattgat caattcgtcc cagggttggg
       61 tgatcaaata agaatttttg tcctgccgga gcaagctgcc tttcctgatc ttgtatagtt
      121 tcatacgggt caaatatagg caattccccg acggtccatc tcaaagatta agcggatttt
      181 aatccatttt acttgcagaa ttcattggtt ttttttactt tatcagacct cgaagaacga
      241 ccttgcttgc ctggcttaac aatgaaacga cacaaaacaa ccccatcggg aaggacttac
      301 ttttcttttc ccgctctttc catccgccag cagctcccgt tgctaatctg cctgttactg
      361 ttagtcctga tcgcgctatt cgggtccatc tcttacctgg gggtccggcg ggcctcgatg
      421 gcggtcgggg aacagcggct acagacactg acggaagagc tttcctcctt atttcagcaa
      481 tccggtcata ccctggcggt tggcacgcaa gccctggcca ggcaggagaa tattgtcaat
      541 tattttaccg gggattctgt aaaccgagtc gcctccgtca aggcgatgga agtattgcaa
      601 aagatgctcg ccgactccca gaccattcgg gcagaggtac gggacctaca gggcaggttg
      661 ctgctgcaag cgggaagggg tgcgtcttcg gtacacgggc ttggttccgc gacagaggcg
      721 aagcggcaat gggcggggcc ggatgtttcg ggagagtttg gcccgggaaa tcgtgatagc
      781 aattttgtag gccggcttta cctggccggc gacagcatgt tttatccgat agtggcccgg
      841 gtgaaccggg gggagcgagc ggaaggctgg cttctccggt ggcgcccggt gagggcgaca
      901 cagaagtccg ttgctcagct gtcccacttg ctgggcgcta aggctacgct ctacttcggc
      961 aataccgatg ggtctttgtg gacggacctg ctaaagcagg tgaccaatac ctcgccggcg
     1021 gtgaaagaac tgcggaagac cctacactat cgggcccagg gcgggaaggt gctcgcttat
     1081 gccatgccga tagcgggaac ccggtggttg atcctggtcg agctctcgga agatctgatc
     1141 ctggaggcgg ccaaccgctt tttatactgg atgctcggca tcggggccgt cctggtaata
     1201 accggaagcc tgatcgcctg gttgatcagc cggaacgtca cccgcaggct ggacaggctg
     1261 atgtgggcca cgaccgttat cgcggaaggg gattattcat cgcaggtgcc tgtagaccgg
     1321 caggatgaga tcggcaggct tgcggcatcg ttcaatacga tgacgatgca ggtgcgtcat
     1381 acccaggaag acctggagga aaaggtcaag ctcaggacgc atgagctgga gaccgtgaac
     1441 aaggagctgg aggccttttc ctattccgtc tcccacgatc tgcgtgctcc gctgagagcc
     1501 gtcagcggtt atgccatgat gttgaaagaa gactatgaac atagttttga cgacgaagcc
     1561 aggcgcatca cgggtaatat cctggcgaat gtaaagatga tggggaggct tatcgacgat
     1621 ctgatcgggt tttcccggtt gggaaagcgc gacgtcagga gacagcatac ccctatgaaa
     1681 gacctggtcg atgtctgtgt cgccgagctg acaccggcct ggcccgaggg gaaattccag
     1741 gtcaacgtcg gtgtattgcc tgcctgcatg ggcgacagcg acctgctcaa gcaggtgtgg
     1801 ctcaatcttg tcagcaatgc gatgaagtat tcttcccgca gggaggaacc gcagatcgct
     1861 atcggcgcca tggaagaagc gggaggaacg gtttatttcg tccgggacaa cggggcgggg
     1921 ttcgatatgc gatatgcgga caaattattc aaggttttcc agcgtctgca cagccaggag
     1981 gaattcgagg gaaccggggt ggggctcgcg ctggtgaaac gaattctgga gaagcaccgg
     2041 ggggagatat gggcagaatc ctcgcccggt aacggggccg ctttttattt caggataccg
     2101 gcctgatcga atataaatta accataccga tatgtttaac cacgtggaag tgctactcgt
     2161 agaagacaat atccatgatg cggaaatgac gatccgcgcg ttgaaaaaag tgaatctcgc
     2221 caataacctg gtccatgtga aggatgggga ggaggcgttg gacttcgtct atgcgcgggg
     2281 cgggttttcc aaccgtcaat ccgccgatct gccaaaggtg a
//
LOCUS       DATGFG010000461         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_237452, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000461 DATGFG010000000
VERSION     DATGFG010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237452"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VKQ52_08550"
     CDS             <1..488
                     /locus_tag="VKQ52_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HLZ87276.1"
                     /translation="GIQKPGLADLHPQTRLTEKIIQCMRTRYVLLALALATFIFAVFR
                     YYNAKGKPENVFQNAAPTDIRFPKTLMDVGAQKYHVPVEARFVVYNVGKKDLFIENVL
                     PDCHCTVADYTKKPISPNDSAVISLKYDARNMGSFQSSAVVSTNSVSSPTLLVFRGFV
                     Q"
     gene            complement(689..1420)
                     /locus_tag="VKQ52_08555"
     CDS             complement(689..1420)
                     /locus_tag="VKQ52_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87277.1"
                     /translation="MNSLEQLSRQVRLLRGFVATLAVIVIGCVIALVLIARRNDRFTE
                     LTVRRINIVEPDGRLALVISDHAAQHPGAMNGKDLPKRDRPAGMIFFNEEGDECGGLV
                     YDGTKRSASMTYSIDQYKNDQIMQIQYEQDSDRSLTRSYGFKLWDRSDRFTLQDEWGY
                     FDSVQRLKDTAAVTAAVKTLRAKGYLGVQRLFLGRTADGETGLFLRDDKGIPRLRICV
                     TKGNQPVIELLDEKGKVLSSGHITP"
     gene            1559..2938
                     /gene="tig"
                     /locus_tag="VKQ52_08560"
     CDS             1559..2938
                     /gene="tig"
                     /locus_tag="VKQ52_08560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HLZ87278.1"
                     /translation="MATVTRENIGLLTDKITVNVSKGDYLPSFEKSLKQYGKQANIPG
                     FRKGMVPAGLIKKMYGNSVFTDEVLRTVEKELTSYMSNEKLEIFAQPLPLPDNDSRQI
                     DINNPADYSFAFEIGLKPAFTLADLGSAKLTRLKVEVTPEMVDEEIERQRNRNGKMTD
                     QEAVNSDENVLNLQFTESDANGQPVEGGIQKDNSLLVKYFNEATRPKWIGLKKDDFLV
                     LQPSQAFEAKEREWVLGDLGLDKNDAAGADKYFKVTITRVGLIEKPELNEEFFKTAYP
                     GKEITTEAAFRDAVKEDIQAYWDNQSRNQLQHTLYHALLDQTKIEFPEDFLKRWMQTG
                     GETPKTQAEVEQEFPSFANQLKWTLIVDKIVHDNNIEVGPDDIRAFAKQQLFGYMGMP
                     GGAEEQPWVADYVQRMMQDRKFVEDTVHRIQTEKVFGWAETQIHPTDKPISKTEFEHL
                     VEEHQHHHH"
     gene            3100..3792
                     /locus_tag="VKQ52_08565"
     CDS             3100..3792
                     /locus_tag="VKQ52_08565"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLZ87279.1"
                     /translation="MNPANEFEKYAVKHRGISSNTLNDYNKYVVTNLTPNIIEERPMN
                     VAVMDVYSRLMMDRIIFLGYPINEEVANIVTAQLLFLDSTDRHRDIQMYINCPGGSVY
                     SGLGVYDTMQYVTPDVSTICIGMAASMGQVLMCAGTKGKRTALKHSRVMMHQPSGAIG
                     GQTTDIEITVNEIKKLRKELYEITAFHTGKTPKQVEKDSDRDYWLTAQEAKDYGLVDE
                     VLLLNPRKEIRE"
BASE COUNT         1034 a         1086 c         1000 g          827 t
ORIGIN      
        1 cgggcataca aaagcccggg cttgctgacc ttcaccctca aacccgttta acagaaaaaa
       61 tcatccagtg tatgcgtaca agatatgttc tattggcttt ggccctcgcc acctttatct
      121 ttgctgtatt cagatactac aacgctaaag gcaagcctga aaatgttttc caaaacgcag
      181 cgcccaccga catccgattc ccgaaaaccc tcatggatgt gggagcccag aaatatcacg
      241 ttcccgtaga ggccagattt gtagtctaca atgtcggaaa aaaggatctc tttattgaaa
      301 atgtgctccc tgattgtcat tgcaccgtcg cagactacac gaaaaaaccc atatcgccca
      361 atgactcggc tgttatcagc ctgaaatacg atgcccgaaa tatgggctcc ttccaatcct
      421 ccgccgtggt gtcgacaaac tcggtgtcat cgccgaccct tctggttttc aggggatttg
      481 ttcaatgatg tcaaatgcag attcttacgt aaagtgatac ctactttttc ttatcgacta
      541 tcccctttgc atccgataac cttcaccgtc aaatcagtaa ggcgacgagt tgtcagtctt
      601 aatagtaatt aatattttaa tatgatattg gcgagctggc gccgcaaggc atgtaggttc
      661 gacccctatc gcccgcactt cttactactc aaggggttat atgccccgat gaaagtactt
      721 tccctttttc atcgaggagc tcgatgacag gctggttccc tttcgtaacg cagatgcgaa
      781 gccgggggat ccccttgtca tcacgcagga acagcccggt ctccccatcg gccgtccgcc
      841 ccaggaagag ccgctgcacg cccagatatc cctttgcccg caatgttttc accgcggcgg
      901 tgacggcggc ggtatccttc agccgttgaa cagagtcgaa atagccccat tcatcctgca
      961 gggtgaaccg gtcgctgcga tcccagagct tgaacccata actccgggta aggctccggt
     1021 cgctgtcctg ctcgtactgg atctgcatga tctggtcgtt cttatactga tcgatggagt
     1081 aggtcatgct cgccgatcgc ttcgtcccgt catagacgag gccgccgcat tcatcccctt
     1141 cctcgttgaa gaagatcatg ccggcggggc ggtcgcgttt gggcaggtcc ttgccgttca
     1201 tcgcgccggg atgctgggcg gcatgatcgc tgatcacgag ggcaagcctg ccgtccggtt
     1261 cgacaatatt gatccggcgg accgtgagtt cggtaaaccg atcgttccgc cgggcgataa
     1321 gaacaagggc gatcacgcaa ccaatgacga tcaccgccag tgtcgcgaca aacccgcgca
     1381 ggagcctgac ctgccgggaa agctgttcta gagagttcat ggtcgtgaat ttagggataa
     1441 ttgtatcaaa tcgaaatact tcgctgaaaa cctaaaaccg gcggtaaaac ccaaaaaatg
     1501 cgtacctttg ccccccgatt tgattttttt tgccctccga tttaaatttt atagcattat
     1561 ggcaacagta acgagagaga atatcggact cctgacggat aagatcaccg tcaacgtctc
     1621 aaaaggcgat tatctgcctt ccttcgagaa atctctgaaa caatatggta agcaagcgaa
     1681 catccccggc ttccggaaag ggatggtgcc cgccggcctc atcaagaaga tgtatggcaa
     1741 ttccgtcttt acggacgaag tgttgaggac cgtggaaaag gagcttacct cctatatgtc
     1801 gaatgagaag ctggagatct tcgcccagcc gctcccgctg ccggacaatg attcccggca
     1861 gatcgatatc aacaaccccg ccgactactc gtttgcattc gagatcgggc tgaaacccgc
     1921 ctttacgctt gccgacctgg gttcggccaa actgaccagg ctgaaagtgg aagtcacccc
     1981 ggaaatggtc gacgaagaga tcgaaagaca gcggaaccgg aatgggaaaa tgaccgacca
     2041 ggaggccgtg aacagcgatg agaacgtcct gaacctgcaa ttcaccgagt cggacgccaa
     2101 tggacaaccc gtcgaaggcg gtatccaaaa agacaattcc ctgctggtaa aatattttaa
     2161 cgaggccaca aggccgaaat ggatcggcct gaagaaggac gacttcctcg ttctccagcc
     2221 gtcacaggcc ttcgaagcca aggagcggga atgggtgctg ggcgacctgg gccttgacaa
     2281 gaacgacgcc gccggcgccg ataaatattt caaggtgacg atcaccaggg tgggcctgat
     2341 cgagaagccc gagctcaacg aagagttctt caagaccgcc taccccggta aggagatcac
     2401 caccgaggcg gctttccgcg atgccgtaaa ggaagatatc caggcctact gggacaacca
     2461 gagccggaac cagctgcagc atacgcttta tcatgccctg ctcgatcaga cgaagatcga
     2521 gttccccgaa gacttcctga aacgctggat gcagacgggt ggcgagacgc cgaagaccca
     2581 ggcagaggtc gaacaggaat tcccctcgtt cgcgaaccag ctgaagtgga ccctcatcgt
     2641 ggacaagatc gttcacgaca acaatatcga agtaggcccc gatgatatca gggcctttgc
     2701 aaagcagcag cttttcggct atatgggcat gcccggcggc gccgaggaac aaccctgggt
     2761 agccgactat gtgcagcgga tgatgcagga ccggaaattt gtcgaggata ccgttcaccg
     2821 tatccagacc gagaaggtct ttggatgggc ggaaacgcag atccacccga cggataagcc
     2881 catcagcaag acggaattcg aacacctggt ggaagagcat cagcaccacc accactagac
     2941 atcgctttta cctccgcccg gcctcgcgcc gggctttttt tggaaccggc ctgctaaact
     3001 gtcagttttt tggcaatttg aaagtttgga cggagatttg aaaggaagtt cccttacctg
     3061 acagatagaa ccggcattac atcattgaaa aatacgatta tgaatcctgc aaacgaattc
     3121 gagaaatatg ctgtgaaaca ccgtggtatt tccagtaata cattaaacga ctacaacaaa
     3181 tacgttgtca ccaatcttac ccccaacatc atcgaagaac gcccgatgaa cgtagccgtc
     3241 atggacgtct acagccgcct gatgatggac cggatcatct tcctcggtta cccgatcaac
     3301 gaggaagtgg ccaatatcgt cacggcgcag ctgctcttcc tcgacagcac cgaccgtcac
     3361 cgggatatcc agatgtatat taactgcccc ggcggcagcg tctattccgg tctcggcgtc
     3421 tacgacacga tgcaatatgt gaccccggat gtgtctacca tctgtatcgg gatggcggct
     3481 tcgatgggcc aggtactgat gtgcgccgga acaaaaggca aacgcacagc gctcaaacac
     3541 agccgcgtca tgatgcacca gcccagcggc gccatcggcg gccagaccac cgatatcgag
     3601 atcacggtca acgagatcaa aaagctgcgg aaggaattgt atgagataac ggccttccat
     3661 acgggcaaaa cgcccaaaca ggtggagaag gacagcgacc gtgactactg gctgacggct
     3721 caggaagcaa aggattacgg cctcgtcgat gaagtgctgc tgctgaaccc aagaaaagag
     3781 atcagggagt agtccctcgc cgaccaaatc gaataagaca ctttcaatca taaaaaaatg
     3841 atcatgaact attccaaata tttgttgaaa cctacccacc tggatgacga gcccgatgaa
     3901 aaggaaccaa aggacgacaa gggtcccgac ctgggcctgc tggccaa
//
LOCUS       DATGFG010000462         4012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_580822, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000462 DATGFG010000000
VERSION     DATGFG010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580822"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VKQ52_08570"
     CDS             <1..671
                     /locus_tag="VKQ52_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87280.1"
                     /translation="LHSLVNKYIRDRIAKQENKAAANAAVTGDEHGDPNAPLPQDDDS
                     LNLLFRHEQNERGMIRVLLEYGLRSWDEQTLVADYVLGESAELADLIDNKRLLSLLQL
                     FRDWYQQGLEPTSKNFLYHEDQELSTLAVSVMDVNLELSPNWKEHYEGKIATPDDLYK
                     EDVTSVIRYLKLRKIKRLMEENQVDMNKPIPHEEWLVLYETHKHLKDMEIALMQGLGT
                     VIVK"
     gene            complement(728..1282)
                     /locus_tag="VKQ52_08575"
     CDS             complement(728..1282)
                     /locus_tag="VKQ52_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ87281.1"
                     /translation="MATFSRQGLVTELLDRTELLKASTQPFLRLTDEQLLLRPGPGRW
                     CIAEVYRHLNLSMDQYIRSILSQITLAPDHPSDVYRSGWLGDRLYGRIMPRADGSVLK
                     LKAVPAHSPGEGVLEGREELEAFQRHCDALDDILRHVGTKDLRRIRIPFFMNGLIRLR
                     LGDLLRFLVAHGERHLLQAQRVQV"
     gene            complement(1486..1995)
                     /locus_tag="VKQ52_08580"
     CDS             complement(1486..1995)
                     /locus_tag="VKQ52_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87282.1"
                     /translation="MSQSLLEKLQLTDERNLLIQGLPSSIEKQFVKLAFAKNLTPLLK
                     SKKIDFALIFAINEQQLRGIVQEVLPALRADGKFWVAYPKPGSKIATTLTRECSWGCI
                     TSMGYEIVRDVAIDHVWTAVRFKKAIPPVSFRAPLRESREPVLEEAVAERVSGTRKPA
                     SRSRRVTSA"
     gene            complement(2072..2737)
                     /gene="plsY"
                     /locus_tag="VKQ52_08585"
     CDS             complement(2072..2737)
                     /gene="plsY"
                     /locus_tag="VKQ52_08585"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HLZ87283.1"
                     /translation="MKEVLLILSAYLIGSIPTAIWVSRSFFGIDIREYGSGNAGATNT
                     FRVLGSRWGCFVMGVDVLKGVIATSLYILVPYYLHDETDRTNFMVGLGLAAVVGHIFP
                     IWAEFRGGKGVATLFGMIIAIQPLAAVCCVAVFLLVLYLTRFVSLSSILASVAFAVLI
                     LYIFDAKETLYRGFAIAVALLVVLTHQKNITRLLRGSENKVPILKYRDRRRHRRNVDR
                     DKE"
     gene            complement(2916..3734)
                     /gene="prmA"
                     /locus_tag="VKQ52_08590"
     CDS             complement(2916..3734)
                     /gene="prmA"
                     /locus_tag="VKQ52_08590"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLZ87284.1"
                     /translation="MVEITITTADWQEVLIALLSESGYEGFEQQDTFLRAYVREEDFD
                     RAALEAVLEPFGLSYAEQRLAERNWNEEWEKNFQPVVVDSFCAIRAHFHAPVEGVEHE
                     LVITPKMSFGTGHHATTFMMIQAMREVDFRGKRVLDFGTGTGVLAILAERLGAAEVVA
                     IDNDDWSIANAQENIEMNGCNNVIVSKIDEIAKVAGPFAVILANINKHVLLRELGEMG
                     QQLKEGGVILLSGLLEDDYNDIENEAVKNNLPISVRMTKGAWICIRSEKKAVQC"
     gene            complement(3789..>4012)
                     /locus_tag="VKQ52_08595"
     CDS             complement(3789..>4012)
                     /locus_tag="VKQ52_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HLZ87285.1"
                     /translation="GKQGLLHISEVSHKRLETMDGVLKEGDMVKVKLIGLDQKTGKFK
                     LSRKVLMPKPEGAGAGSPKAPAGGRPEHE"
BASE COUNT          975 a         1268 c          943 g          826 t
ORIGIN      
        1 gcctccacag cctcgtcaac aaatatatcc gggacaggat cgccaaacag gagaacaagg
       61 ccgccgccaa cgccgccgtc acaggcgatg aacacggcga tcccaatgct cccctgcccc
      121 aggacgacga ctcgctcaac ctgctcttcc gccacgaaca gaacgaacgg ggaatgatcc
      181 gcgttctcct ggaatacggc ctccggtcct gggacgagca gaccctcgtc gccgactatg
      241 ttctgggaga atccgccgaa ctggccgacc tcatcgacaa taaacgcctc ctctccctcc
      301 tccagctctt cagggactgg tatcagcagg gcctcgaacc aacctccaag aacttcctgt
      361 accacgaaga ccaggaactc agcaccctcg ccgtctcggt gatggatgtc aatctggaac
      421 taagccccaa ctggaaagaa cattacgagg gaaagatcgc gacccccgac gacctttaca
      481 aggaagacgt cacatccgtc atccgctacc tcaaactgcg aaagataaaa agattgatgg
      541 aagagaacca ggtcgacatg aacaaaccca tcccccatga agaatggctg gtgctgtatg
      601 aaacgcacaa gcacctgaaa gatatggaga tcgccctgat gcagggtctt ggcaccgtca
      661 tcgtcaagtg aaccgccggt atcggatgac cggcgccacc cgcttgccgg cccgtcaggc
      721 ccgaagccta cacctgaacc cgttgcgcct gcagcagatg ccgctcccca tgcgccacca
      781 ggaaccgcaa cagatcccca aggcgcagcc ggataagccc attcataaag aaggggatcc
      841 tgatccgccg caggtccttc gtcccgacat gccgcaaaat atcatccagc gcatcacaat
      901 gccgctgaaa tgcctccagc tcctcccgcc cctccaatac cccctcgccg ggagaatgcg
      961 ccggcacggc ctttaatttg agcacgctgc catcggcccg gggcatgatc cgcccataca
     1021 accggtctcc cagccagccg ctgcgataca catccgaagg atggtccggc gccagcgtga
     1081 tctgcgaaag gatactccgg atgtactgat ccatggaaag attgagatga cggtagacct
     1141 cggcaataca ccatctgccg ggacccggcc ggagaagcag ttgctcgtcc gtcaggcgaa
     1201 gaaagggttg cgtactggct tttagcagct cagtcctgtc cagtaactcc gttaccagac
     1261 cttgcctgct aaaggttgcc atggggaaaa ggggtaagat aaaagcatat cgttgtcgta
     1321 gcgctaaaga tacgcgaacc ccctgttggt ggcgaatttc agagaggaaa atacccaaag
     1381 accctgagcg ctcatccccg gagagaggat attctgcgcc ccccgcgcag gcgaagcccg
     1441 gccgatgcgc cagagctgcg gtcggatacc ctgctgatca cctggttagg cactggtgac
     1501 acgccggctg cggctggccg gcttccgggt gccgcttacc cgttccgcaa cggcctcttc
     1561 cagcacgggc tcgcgcgatt cgcgaagcgg agcccggaaa ctcaccggcg gaatggcctt
     1621 cttgaagcga acggccgtcc atacatggtc gatcgctaca tcccggacga tctcataccc
     1681 catactcgtg atacagcccc agctgcattc ccgtgtaagc gtggtcgcga tcttcgatcc
     1741 gggcttggga taggccaccc agaatttccc gtcagcccgc aacgcgggaa gcacttcctg
     1801 cacgatccct ctcaactgct gttcgttgat cgcaaagatc agcgcaaaat ctatcttctt
     1861 gctcttgagc aagggagtaa gattcttggc gaaggctaac ttgacgaatt gcttttcgat
     1921 ggaagaaggc agaccctgta tcaacaggtt tctttcatcg gtcaactgta gtttttccag
     1981 taaactttga gacattacta cgcctgttta tagtttctga tatgagcgag acagaccgca
     2041 ttacgcatgc gggatggcaa tattagcgaa tttattcttt atctctgtcg acattcctcc
     2101 ggtgccggcg ccggtcgcga tatttaagga taggaacctt gttctcactc ccccggagca
     2161 ggcgggtgat attcttctgg tgggtaagga cgacaagaag ggctacggca atggcaaacc
     2221 cccggtaaag ggtctccttc gcgtcgaaaa tataaagaat gagaacggca aaagctacgg
     2281 acgcgagtat gctgctaagc gaaacgaaac gggtaagata cagcaccagc aggaacaccg
     2341 ccacacaaca gacagccgcc agcggctgga tcgcaatgat catcccaaaa agcgtcgcta
     2401 cccccttccc gccgcgaaac tcggcccaga tggggaaaat atgaccgaca acggccgcca
     2461 gacccagtcc gaccataaaa ttggtccggt cggtctcgtc atgtaaatag tagggaacaa
     2521 gaatgtaaag actcgtggcg ataaccccct tcaacacgtc cacacccatc acaaaacaac
     2581 cccaccgcga acccaacact ctgaaggtat tggtggcacc ggcattacca ctgccatatt
     2641 cgcggatgtc tattccaaaa aacgatcggc tgacccagat agcagtagga atagaaccta
     2701 tcagatacgc cgatagaatg agtaaaacct ccttcattcg aatggttgag ttggtgcacc
     2761 gggactggtt ttctgccgaa atatacccat tcccggtgca cggctaataa tgggccttcg
     2821 gccagcggac ccgtggtccg cgttggttgg ataacaaata taccgaaaaa aagactgcga
     2881 tccattcgcc ttccgccact taatttcatc tttttttaac attggaccgc ctttttctcc
     2941 gatcttatac agatccaggc acctttcgtc atccgtacgg atattggtaa attattctta
     3001 acggcctcat tttcaatgtc attatagtca tcttccaaaa ggccgctcaa tagtataacg
     3061 cccccctctt tcaattgttg ccccatttcg cctaactccc ggagaagtac atgtttgttt
     3121 atattggcaa ggatgacggc aaacggcccg gcgaccttgg caatttcatc tattttgctc
     3181 actatcacat tattacaccc attcatctca atattttcct gagcattggc aatcgaccag
     3241 tcatcattgt cgatcgccac cacctccgcc gccccaagcc gctccgccag gatggccagc
     3301 acccccgttc ccgtaccgaa atcgagcacc cgcttccccc ggaagtccac ctcccgcatc
     3361 gcctgtatca tcatgaacgt cgtcgcatga tgtcccgtgc caaaactcat cttgggcgtg
     3421 atcaccaact catgctccac accctccacc ggtgcatgga aatgcgccct gatcgcacaa
     3481 aacgaatcga ccaccaccgg ttgaaaattc ttctcccact cctcgttcca gttccgctcc
     3541 gccaggcgct gctccgcata ggacaatcca aatggctcca gcacagcttc cagcgccgcc
     3601 cggtcaaaat cctcttcccg cacataggcc cgcaaaaaag tatcctgctg ctcgaatccc
     3661 tcgtaacccg attcactcag cagggcgatc agcacctcct gccaatcagc cgtcgttatc
     3721 gttatctcta ccataaaaaa aagtctgcgg ctcgcaccgc agactggtat tttggtttac
     3781 aatgatcttt actcatgctc gggacgaccg ccggcaggcg ccttcggcga acccgcacca
     3841 gccccctcgg gcttcggcat cagcaccttg cggctcagct tgaacttccc ggtcttctgg
     3901 tccagaccga tcagtttcac cttcaccata tcgccttcct tcagaacgcc atccatcgtc
     3961 tccagccgtt tgtgcgagac ctcgctgata tgcagcagac cctgcttccc gg
//
LOCUS       DATGFG010000463         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_795781, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000463 DATGFG010000000
VERSION     DATGFG010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795781"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2974
                     /locus_tag="VKQ52_08600"
     CDS             <1..>2974
                     /locus_tag="VKQ52_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87286.1"
                     /translation="ARKILDYQVSVVAGKKAKAFFVPGNHDWKQGKVGGWQQVKNEEA
                     YLHGLGMPNVDLLPRDGCPGPVAVPLGNKMVLVCMDSQWWLQDKQDRPGLESACDCKD
                     ERSIINSLNDIVSLYPDRLIVLAMHHPLYTHGEHGGYYTLKQHIFPLTDLKSGLYIPL
                     PVIGSIYPVSRGVFGNVQDVKNPRYKNLRDQVEAVIRGHNNIIDVAGHEHTLQLLQHD
                     SVYYVVSGAGSKNTRVRMGRHSLMARENIGFATIELHASGRSEIRFYNVDAKDLDQTF
                     YTAAVPPLIPHVDTTALVRSFPDSVTAVADSEFLAGGFKRWLLGANYRKEWSAPVKVK
                     VFDLTGWTPVQRGGGSQTRSLRIVNVEGKQYVLRGVKKYVSDIALPTALQGDQFVRDI
                     VSDGVSASYPYACMSIPPLAQALGVPHAHPQLVYVPDDPRLGRFRADYGNLFAFVEEH
                     EPGNGKKTYNMWDIESLLREDNDNTVDQRATLRARMLDMFVMDFDRHEDQWRWMAEDN
                     GKGKTLSPVPRDRDQPFFINNGFITGIVGSAWAVPQLQGFRAKARNIYGLNYAPRNFD
                     RNYLNELTEKEWRATAEEVVGKMTDSLIESAMRLQPLPIQGDAMHSIIARLKERRNYF
                     VGEMMNYYAFISRMVSVYGSDKRELFDVERRSGDSVIVTVYKLNKEGEAGKRLYSRVF
                     LSGMTHEIRLYGQGGDDQFHIHGGGGGGTVVRIIGGPGSDSYRNEVHAPAGKTKIYDL
                     STEKNVFEGSGAERKFLSTDPAVNEVVRMGYIGNSGLGFHYNILAPLLSVAYNPDDGV
                     FIGAGFRYTTNGFHKEPYKTLQTLTAEHSLSTKAYAFRYALEATKAIGSLDLLVHADV
                     KAPNNTINFFGFGNETVFNRQAKGGIRYYRTRYNSFDLDLQLRKRFGSVFSIAAGPVL
                     QYFSLDSAENFDRFINQTRLNGLDPAQLYRDRSYAGGRVSVIVDDRNDKILPSRGIFW
                     QTQFGSFGGLNDASHAYSR"
BASE COUNT          618 a          676 c         1036 g          644 t
ORIGIN      
        1 ggcgcggaag atcctggatt accaggtgtc ggtcgtggcg gggaagaagg cgaaggcctt
       61 ttttgtgccg ggcaatcatg actggaagca ggggaaggta ggcggctggc agcaggtgaa
      121 gaacgaggag gcctatctcc atggccttgg gatgccgaat gtggacctgt tgccccggga
      181 tggctgtccg gggccggtgg cggtgcccct gggcaataag atggtgctgg tatgtatgga
      241 cagtcagtgg tggctgcagg ataagcagga ccggccgggg ctggaatctg cctgtgactg
      301 caaggatgag cgatcgatca tcaattcgtt gaatgatatc gtgagcctgt atcccgaccg
      361 gctgatcgtg ctggcgatgc atcacccgct ctatacgcat ggggagcatg gcggttacta
      421 taccctcaag cagcatatct tcccgctgac cgacctgaag tccgggcttt atattccctt
      481 gccggtgatc gggtcgatct accctgtatc gcggggggta ttcgggaatg tgcaggatgt
      541 gaagaatccg cggtacaaga acctgcggga ccaggtggag gccgttatcc gggggcataa
      601 caatatcatc gatgtagcgg ggcatgaaca tacgctgcag ttgttgcagc atgacagcgt
      661 ctattatgta gtgagcgggg cgggttcgaa gaatacgcgg gtgaggatgg gcaggcattc
      721 gctgatggcc agggagaata tcgggtttgc gacgatcgag ctgcatgcga gcggcaggag
      781 cgagatccgg ttctataacg tggatgcgaa ggatctcgat cagacctttt atacggcggc
      841 ggtgccgccg ctgatccccc atgtcgacac gacggcgctg gtccggagct ttccggacag
      901 tgtaacggcg gtggccgatt cggaattcct tgccggcggg ttcaagcgat ggctgcttgg
      961 ggccaattac cggaaggaat ggtcggcgcc ggtgaaagta aaggtgtttg acctgacggg
     1021 ttggacgccg gtgcagcggg gtgggggaag ccagacgcga tcgctgcgga tcgtgaatgt
     1081 cgagggcaaa caatatgtgt tgaggggcgt gaagaaatat gtctccgata ttgcgttgcc
     1141 gacggccctg cagggcgatc aatttgtccg ggatatcgtg agcgatgggg tatctgcttc
     1201 gtatccgtat gcgtgtatgt ccataccgcc gctggcgcag gcccttggtg tgccgcatgc
     1261 ccatccgcag ctggtgtatg tgccggatga tccgcggctg gggaggttca gggcggacta
     1321 tggcaatctc tttgcctttg tggaagaaca tgagcccggc aatgggaaga agacctacaa
     1381 catgtgggat atcgagagcc tgttgcggga ggacaatgat aataccgtcg atcagcgggc
     1441 gacgttgcgg gcgaggatgc tcgatatgtt cgtcatggac ttcgaccggc acgaggatca
     1501 gtggcggtgg atggcggagg acaatggaaa ggggaagacg ctgtcgcctg ttccgcggga
     1561 cagggaccag cctttcttca tcaacaatgg gtttatcacc gggattgttg gttcggcctg
     1621 ggctgtgccg cagctgcagg gcttccgggc gaaggccagg aatatctatg gactgaatta
     1681 tgcgccgcgc aatttcgacc ggaattacct caacgagctg acggaaaaag agtggcgagc
     1741 cacggcggaa gaggtggtgg gcaagatgac cgattccctg atcgaatcgg cgatgcggtt
     1801 gcagccgttg cctatccagg gcgatgccat gcattccatc attgccaggc tgaaggagcg
     1861 gcggaactat tttgtcgggg agatgatgaa ctactatgcg tttatttcca ggatggtgag
     1921 tgtatatgga agcgacaagc gggaactgtt cgatgtcgaa aggcgcagcg gggattcggt
     1981 gatcgtgacg gtctataagt tgaataaaga aggggaggcg ggtaagcggc tctatagccg
     2041 ggtctttctc tccgggatga cccacgagat acggctatat gggcaggggg gtgatgacca
     2101 gtttcatatt catggcgggg gcggcggcgg aacggtggtg aggatcatcg gcggaccggg
     2161 tagtgacagc tatcgtaacg aggtgcatgc accggccggc aaaacgaaga tctacgacct
     2221 gagcacggag aagaatgtct tcgaaggcag tggcgcggag cgaaaattcc tgtccacgga
     2281 cccggcggtg aatgaagtgg tgcggatggg ctatatcggt aactccggac ttgggtttca
     2341 ttataatatc ctggcgccgc tgcttagcgt tgcgtataac ccggatgacg gggtatttat
     2401 cggtgcgggt ttccggtata cgacgaatgg gtttcataag gagccctaca agacgctgca
     2461 gacccttacg gcggagcact cgttgtcgac gaaggcgtat gccttccgtt atgcgctcga
     2521 ggcgacgaag gcgatcgggt cgctggacct cctggtgcat gccgatgtga aggcgccgaa
     2581 caatacgatc aatttctttg ggtttgggaa tgaaacggtg tttaacaggc aggcgaaggg
     2641 tgggatacgg tattaccgga cgcggtacaa cagtttcgat ctcgatctgc aactgcggaa
     2701 gcggttcggg tcggtgttct cgatcgcggc ggggccggtg ttgcagtatt tctcgctgga
     2761 cagtgcggag aatttcgacc ggttcatcaa ccagacccgg ctgaacggat tggatcccgc
     2821 gcagctttac cgggacagga gttacgccgg tgggcgggtg tcggtgatcg tggatgaccg
     2881 gaatgataag atcctgcctt cccggggtat tttctggcag acgcagttcg ggtcttttgg
     2941 gggattgaac gatgccagtc atgcctattc gcgg
//
LOCUS       DATGFG010000464         9455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_254168, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000464 DATGFG010000000
VERSION     DATGFG010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254168"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VKQ52_08605"
     CDS             complement(<1..704)
                     /locus_tag="VKQ52_08605"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HLZ87287.1"
                     /translation="MKQVIYESLLAKKRRGQRSFAVLIDPDNVNAEKIEELTSLAVSA
                     KVDYLFVGGSLVISNHLDEVVQHIRSNCSIPVILFPGTPSQVSPFADGLLYLSLISGR
                     NPELLIGQHVISAPAVRKSGLEIISTGYMVIDGGAPTTVSYISNATPIPADKNEIALC
                     TAMAGEMLGMKLIYMDAGSGARHPIREEMIACVAGHIEVPLVVGGGIRDPEKAYRNCK
                     AGADIIVVGNAIEKDAS"
     gene            857..1144
                     /locus_tag="VKQ52_08610"
     CDS             857..1144
                     /locus_tag="VKQ52_08610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ87288.1"
                     /translation="MEFLNVINNTQQQQFQLPVNGEIAWLEYRFSDGMIVLMHTDVPD
                     QLSGKGIGSALAAAAFDYAKNHHLKVKVYCPFVKTWLARHPDLLPLVVNPS"
     gene            complement(1139..1846)
                     /locus_tag="VKQ52_08615"
     CDS             complement(1139..1846)
                     /locus_tag="VKQ52_08615"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HLZ87289.1"
                     /translation="MLIITLLIFALAGPASSQTQFMGWAATFQNYKVTDKFGLYFDAQ
                     WRSTAQVRQAHALLLRPGINFYFSPSFTGAVGYAFIPQQRISAGVTGYLPEHRVWEQL
                     VFTHKVKTPAGHSATATVTHRLRLEQRYLPKHHAEGNELVKDGHRYAGRVRYFARGVL
                     PLAAQKDKGPFAALQNEIFFHTADGGSFDQNRAYVAAGYRMSRQFDVEMGYMNQYVAG
                     SSPVNNHILQVATYIRL"
     gene            2037..5429
                     /locus_tag="VKQ52_08620"
     CDS             2037..5429
                     /locus_tag="VKQ52_08620"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HLZ87290.1"
                     /translation="MAAKKPTSKGLQFLRRFTKENTNVFDQFSYDYRTSVIRNPSGEV
                     VFEMNNVEVPAQWSQIATDILAQKYFRKAGVPQPDGSLGRETSVKQVAHRMANCWRVW
                     GERYGYFASEKDAQIFYEELVYSILDQACVPNSPQWFNTGLHESYGITGKPQGHYFVD
                     PADGQLKRSTSAYERPQPHACFILSVEDDLVNEGGLMDLWVREARIFKYGSGVGTNFS
                     HIRGEGEKLSGGGTSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPEIMEFI
                     NWKVEEEKKVAALIAAGYPSDYEGEAYRTVSGQNSNNSIRIPNSFFDKLKNDENWELT
                     ARSTGKVMKSIPAREIWNQISYAAWRCADPGTQYETTINEWHTCPAGGPIRASNPCSE
                     YLFLDNTACNLASFNLRRFYNEKTNTFNVEDFEYSARLWTTVLEISVLMAQFPSKEVA
                     QLSYDYRTLGLGYANLGSMLMIMGIPYDSEEARAIAGAITAIMTGVAYKTSAEMAGAL
                     GAFPRYEENRESMLRVMRNHRLAAYDADEYEGLATKPQGLKAKYCPDYLLKAATKAWD
                     EAVQLGEKFGYRNAQSTVIAPTGTIGLVMDCDTTGVEPDFALVKFKKLSGGGYFKIIN
                     QSVPTALKNLHYTDKQIDTIVKYAVGSGTFAGAPAINHQSLSEKGFIAEEIKKLDAAV
                     GSAFEIGFVFNVYTLGEECLQRLGFKPAQYYNFEWSLLEALGFTDEEIEAANNYVCGT
                     MTVEGAPFLKEEHLAVFDCANKCGKKGRRYIHAHGHIRMMGATQPFISGAISKTINLP
                     NEAAVEEIADAYLLSWQLALKACALYRDGSKLSQPLSTRSDKKKKETETEKDTAPAQE
                     AAAIPQPAESTIVDLGKLTVQELLEEVQKRVQASPDTKLKRALATIVERRTLPAKRRG
                     FTQKAKINGQAIFLRTGEYSDGTVGEIFIDMAKEGATMRSMLNCFAIAISIGLQYGVP
                     LEEFVEKFVFTRFEPSGMVDHPNIKSTTSIVDFIFRSLAYEYLGRTDLVHVLDRPEVA
                     NTGSEDWDPADADKKPELSDVRIVAKAGGNAPSGASQPQPAKAPRATASVTRADNGLE
                     AMNAAARSMQSDAPACNTCGHITIRSGTCYKCLNCGNSMGCS"
     gene            5748..5870
                     /locus_tag="VKQ52_08625"
     CDS             5748..5870
                     /locus_tag="VKQ52_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87291.1"
                     /translation="MKRFFVAIVILSSTLLFSCSRSVTPDQAASNHYSHCRDMR"
     gene            6016..6492
                     /locus_tag="VKQ52_08630"
     CDS             6016..6492
                     /locus_tag="VKQ52_08630"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HLZ87292.1"
                     /translation="MTIQFGYNKKQVLDGLRGHFFSRPEIRTMVIMINVFAILSAILF
                     ALKKIQALPFLIFSVLWFSLWISVRRLLPLSIYKRSATFQDTFTLSLEEGGIVLETKK
                     GRQRWSWEDFTAFKETIYFFHVYFSPRSFFMIPKDSFKDITEIQAARKLLKERIGK"
     gene            complement(6494..7516)
                     /locus_tag="VKQ52_08635"
     CDS             complement(6494..7516)
                     /locus_tag="VKQ52_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87293.1"
                     /translation="MRSYVFFLAVCWGLVSCGSADKPDVSGVDVGKVHIERFDTAFFN
                     LDSNDIVQGLRRLTRHYPYFTPDFVGNILGAGGPLTDTSKVAFAATREFLVSYLSVSD
                     SLKVKYPRLDWLEDQLQQDFKYVKYYFPEYKLPQKVVAFVGPFDGPGVAITTYSLAIG
                     LQSYAGRNFPFYLTGKGQDLYPQYVSRRFEPEYIQANCMNAIAQDIFPDSSDNRPLIE
                     EMIMKGRYFWLAGKLLPDVPDSIRTGYSKAQLDWCAGNEAAIWNFFLQGTDLYTIDPD
                     ILKNYIGEGPKTLGMPDAAPGNIGAWVGWQIVKKYAAAHTDLKPARLMTMPVRTIFEE
                     ARYKPK"
     gene            complement(7513..7866)
                     /locus_tag="VKQ52_08640"
     CDS             complement(7513..7866)
                     /locus_tag="VKQ52_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HLZ87294.1"
                     /translation="MAFNERLAQRIREALAHLPDVVEKKMFHGICFMVKGKMCLCVRL
                     DEMLCRIGPHKYAEALEMNHCRPMIHNGRTMTGFVFVSEEGIRKKKDFEYWVNLALAF
                     NSEAKASKKVVKKKK"
     gene            7947..>9455
                     /gene="nadE"
                     /locus_tag="VKQ52_08645"
     CDS             7947..>9455
                     /gene="nadE"
                     /locus_tag="VKQ52_08645"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HLZ87295.1"
                     /translation="MKIALAQQNYHIGNFEENTRKIIAGIGQAKAAGADLVVFTELSV
                     CGYPPRDFLEFDDFIAQCYAAIDHIREHADTIGVIVGAPVRNPVREGKDLFNGAWLLY
                     EKEIKGVAHKTCLPNYDVFDEYRYFEPAYEWKVIPFKGKKIALTICEDIWNLGDNPLY
                     RVCPMDHLIRQHPDVMINISASPFNYDRDEDRKEVVRQNVLKYNLPMYYCNAIGSQTE
                     IVFDGSSLVYDAKGNLVHEMRSFEEDFAVVSLPHPVPPLFEELLPGLSVRSDADDEDA
                     DISGLPDNPATPQAPATTTIAPAVFDKDMQVSKQYDPHRILEYLTDDRNIRQIRKALV
                     LGIRDYFSKMGFSKAIIASSGGIDSAVTLALACEALGKDNVRAILLPSAYSTAHSVGD
                     AEQLSRNLGNPYDIIPIREVYDALLHTLEPVFKDTAFGLAEENLQSRARGNLVMALAN
                     KFGYVMLNTSNKSELSTGYGTLYGDMAGGLSVLGDVYKGQVYALARDINRGGE"
BASE COUNT         2384 a         3016 c         2176 g         1879 t
ORIGIN      
        1 gaggcatcct tttcgatggc attgcccaca acgatgatat cggccccggc cttacagttg
       61 cggtaagcct tctccgggtc acggatcccg ccgccaacga caagcggaac ttcgatatgc
      121 ccggcaacac aggcgatcat ctcctcccgg atgggatgcc gcgccccact gcccgcatcc
      181 atatagatca gcttcatccc aagcatctcc cccgccatgg ccgtgcagag cgcgatctca
      241 ttcttgtcgg cgggaatagg cgtcgcattg ctgatatagg agacggtcgt cggcgccccg
      301 ccatcgatga ccatatagcc ggtggaaatg atctccagcc cgctcttgcg gacggcgggc
      361 gcggagatga catgctgccc gataagcagt tccgggttac gacccgagat cagggataaa
      421 taaagaagcc cgtcggcaaa aggtgacacc tgcgacggcg tacccggaaa aaggatgacg
      481 ggaatggaac agttactgcg gatatgctgt accacctcat cgagatggtt ggatatgacc
      541 aggctgcccc cgacaaataa atagtccacc ttcgcactga cagccagact cgtcagctct
      601 tcaattttct ctgcattcac attgtccgga tcgatcagta ccgcgaagga tcgctgcccc
      661 cttcttttct ttgccaacaa ggattcataa ataacttgct tcattcgctg gtgccgttgg
      721 tgatacaaaa atgcgaaaag tttctggctt ttaagggggt attcgacgaa attaccatct
      781 cggggaatac gtatgtattt attcgtaaaa tccctgaata tttacgaaaa taacacatat
      841 atccgatttt tgcagaatgg aattcctcaa cgtaataaac aatacgcagc aacagcaatt
      901 tcagttgccc gtgaatggcg agatcgcctg gctcgaatat cgcttttcgg atgggatgat
      961 cgttctcatg cataccgacg tgccggacca gttgagcggc aaaggcatcg gctcggcatt
     1021 ggccgccgcg gctttcgatt atgcaaaaaa ccatcacctg aaagtaaagg tttattgccc
     1081 atttgtaaag acctggctcg cccgccaccc cgatctgctg cccctcgtcg tcaatccgtc
     1141 ataaccggat ataggtggca acctgtagaa tatgattatt gacgggactg ctccccgcga
     1201 catactggtt catatacccc atctcgacat cgaattgcct gctcatgcgg taccccgccg
     1261 caacatacgc ccggttctga tcaaaggaac ccccatccgc cgtatgaaaa aagatctcgt
     1321 tctgcaacgc ggcaaaaggc cccttgtctt tttgcgccgc caacggcaga acaccccgcg
     1381 caaaataccg gaccctgccc gcataccggt gcccatcctt caccagttca ttcccctccg
     1441 catgatgctt cggcagataa cgctgctcca gcctcaaccg gtgcgttacc gtagccgttg
     1501 ccgaatgccc ggcaggcgtc ttcaccttat gcgtaaagac cagctgctcc catacccggt
     1561 gctcgggtag ataaccggtc accccggcgc ttatacgctg ctgtgggatg aaggcataac
     1621 ccaccgcccc ggtgaacgaa ggcgaaaagt agaaattgat ccccggtcgc aacaacaagg
     1681 catgcgcctg tcggacctgc gcggtcgatc tccactgcgc atcgaaatag agcccgaatt
     1741 tgtcggtaac tttgtagttc tggaaagttg cggcccaccc cataaattgg gtctggctgc
     1801 tcgccggacc cgcaagggca aaaatcagga gagtaatgat tagcaaggtt ttatttggtg
     1861 ttttcatgca tcaaaaagaa tcaataaaaa gcaaacaaaa tggtaaaatt ttttactcac
     1921 atctatgtgc agaaatagct caaggaaatg tccgaatgaa aaaatacttt taattcccaa
     1981 ccctcccgaa aacaaaacct gacaataact ttcttcacca actaaatgaa ctgatcatgg
     2041 ccgccaaaaa gccgacatcg aagggcttgc aattcctccg ccgctttaca aaagagaaca
     2101 caaatgtatt cgatcagttt tcctacgatt accgcacatc ggtgatccgg aaccccagcg
     2161 gagaagtggt gtttgagatg aacaatgtcg aagtacccgc ccagtggtcc cagatcgcaa
     2221 ccgatatcct cgcccagaaa tacttccgca aagccggtgt gccccagccg gacggctccc
     2281 ttggccggga gacctccgta aaacaggtgg cgcaccgcat ggccaactgc tggcgggtat
     2341 ggggtgaacg ctatggctat tttgcatccg aaaaagacgc acagatcttc tatgaagaac
     2401 tcgtgtacag cattctcgac caggcctgcg tacccaattc cccacaatgg ttcaacaccg
     2461 ggctccacga aagctacggc atcaccggca aaccccaggg ccattacttc gtagaccccg
     2521 ccgacggcca attgaaaaga tccacctcgg cctacgaacg cccccaaccc cacgcctgct
     2581 tcatcctcag cgtggaagac gacctcgtca acgaaggcgg cctcatggac ctctgggttc
     2641 gcgaagcccg catctttaaa tatggctcgg gcgtgggcac caacttctcc catatccgcg
     2701 gcgaaggcga gaaactctcc ggcggcggaa cctccagcgg tctcatgagc ttcctcaaga
     2761 tcggcgatcg cgcggcaggc gccatcaaat ccggcggcac cacccgcagg gccgcaaaaa
     2821 tggtctgcct tgacctcgat cacccggaaa taatggaatt catcaattgg aaggtcgaag
     2881 aagaaaagaa agtggccgcc ctcatcgccg ccggttatcc cagcgactac gaaggcgaag
     2941 cctatcgcac cgtctccggc cagaactcca acaactccat ccgcatcccc aatagcttct
     3001 tcgataaact aaagaacgac gagaattggg agctcacagc ccgcagcacc gggaaagtca
     3061 tgaagtccat ccccgcccgg gaaatatgga accagatctc ctacgccgcc tggcgctgtg
     3121 ccgaccccgg cacccaatac gaaaccacca tcaacgaatg gcacacctgc cccgccggcg
     3181 gccccatcag ggcgtctaat ccctgcagtg aatacctgtt cctcgacaac acggcctgta
     3241 acctcgcctc cttcaacctc cgccgcttct acaacgaaaa gacaaacacc ttcaacgtag
     3301 aagacttcga atactcggcc cgcctctgga ccaccgtcct ggagatctcc gtcctgatgg
     3361 cgcaattccc ttcgaaagaa gtagcccagc tgtcctacga ttaccgcacc ctgggcctcg
     3421 gctacgccaa cctgggatcc atgctgatga tcatgggcat cccctacgac agcgaagaag
     3481 cccgcgccat cgccggcgcc atcaccgcga tcatgaccgg cgtcgcctat aagacctccg
     3541 ccgaaatggc cggcgccctc ggcgccttcc cccgctacga agagaatagg gaaagcatgc
     3601 tccgcgtcat gcgcaatcac cggcttgccg cctacgacgc cgacgaatac gaaggcctgg
     3661 cgacaaaacc ccagggcctg aaagcaaaat attgccccga ctacctcctg aaggccgcta
     3721 caaaagcctg ggacgaagcc gtacaactcg gcgagaaatt tggctaccgc aacgcccagt
     3781 ccaccgtcat cgcccccacc ggaacgatcg gcctggtcat ggactgcgac accaccggcg
     3841 ttgaacccga cttcgccctc gtgaaattca aaaaactctc cggcggcggc tacttcaaga
     3901 tcatcaacca gtcggtaccc acagccctga agaacctgca ttacaccgac aaacagatcg
     3961 ataccatcgt aaaatacgcc gtcggctcgg gaaccttcgc cggcgccccc gcgatcaacc
     4021 accaatccct gagcgaaaaa ggcttcatcg ccgaagagat aaaaaaactc gacgccgccg
     4081 tcggctccgc cttcgagatc ggcttcgtat tcaacgtcta taccctcggc gaagaatgcc
     4141 tccagcgcct cggcttcaaa cccgctcaat actacaactt cgaatggagc ctgctcgaag
     4201 ccctcggctt caccgatgaa gagatcgaag ccgccaacaa ctacgtctgc ggcaccatga
     4261 ccgtcgaagg cgcccccttc ctgaaggaag aacacctcgc ggtcttcgac tgcgccaaca
     4321 aatgcgggaa aaaaggccgg cgctatatcc acgcacacgg tcatatccgc atgatgggcg
     4381 cgacccaacc cttcatcagc ggcgcgatct cgaagacgat caacctgccg aacgaagccg
     4441 ccgtcgaaga gatcgccgac gcctacctgc tgagctggca actggccctc aaagcctgcg
     4501 ccctctatcg cgacggctcc aaactgtccc aacccctcag cacaagatcc gacaagaaaa
     4561 agaaggaaac cgaaacagag aaagacaccg ccccggccca ggaagccgcc gccatacccc
     4621 agccggccga atccaccatc gtcgacctgg gcaaactcac cgtacaggaa ctcctcgaag
     4681 aagtacaaaa acgcgttcag gcatcccccg atacaaaatt gaaaagggcg ctggctacca
     4741 tcgtcgaacg ccgcaccctg ccggcaaaac gccgcggctt cacccaaaaa gcaaagatca
     4801 acggccaggc catcttcctc cgtaccggtg aatacagcga cggaacagta ggcgagatct
     4861 ttatcgacat ggccaaggaa ggcgcaacca tgcgaagcat gctcaactgt tttgccatcg
     4921 ccatctccat cggcctccaa tacggcgtgc cactggaaga gttcgtggag aaattcgtct
     4981 tcacccggtt cgaaccctcg ggtatggtcg accaccccaa tatcaaaagc actacctcca
     5041 tcgtcgactt catcttccgc tccctggcct atgaatacct gggacgcacc gacctcgtac
     5101 acgtgctcga ccggccggaa gtcgccaata caggcagcga agactgggac cccgccgacg
     5161 ccgataaaaa gccggaactc agcgatgtac gcatcgtggc caaggccggc ggcaacgctc
     5221 cctcaggcgc ctcccagcct cagcccgcaa aagccccccg ggccacggct tcggtaaccc
     5281 gtgccgacaa cggcctggaa gccatgaatg cagccgccag gagcatgcaa agcgacgccc
     5341 cggcctgtaa cacctgcgga catatcacca tccgcagcgg aacctgctat aaatgcctca
     5401 attgcggtaa cagcatgggt tgcagctgac cgtagggcat tctcaacttc gtctaaggac
     5461 ggtatttaac cataaattcg attcccccat gtccatgggg gaatttcttt ttcctggccc
     5521 ctttcaacca cagctcaaac ccaaaatttg acaatttcga cctttccggg tcaggattgt
     5581 ccccctcccg gggccccttc agaaaaaccg ttattttttc ccggcacttc ttcgtacctt
     5641 ttccatagaa acaacgcaaa aaatatacag ccttacccct acgaaaaaat actaatatct
     5701 gcttttattc gtttttaata agtcctgtaa accctaaatt gaaaaacatg aaacgttttt
     5761 tcgtcgccat cgtcatcctt tccagtacat tattgttttc ctgtagccgt agcgtgaccc
     5821 ccgaccaggc cgccagtaat cactatagcc attgccggga catgcgctag cgtaccacaa
     5881 aaaaaatttt ttgaagcctc cggcgaaagc cgggggcttt tttttgcccg ggcggtcaca
     5941 ggcggcatcc attttttctt acttttaccc gcaataaaat ggaccgcgaa aggtctgaat
     6001 gttaaaactt cggggatgac tattcaattc ggatacaaca aaaaacaggt attggatgga
     6061 ttacgtggcc actttttcag cagaccggag atccgaacca tggtcatcat gatcaacgtt
     6121 ttcgccattt tgtcggctat cctattcgca ctcaagaaga tacaagcctt gcccttcctg
     6181 atcttctccg tcctctggtt ctccctgtgg atctccgtcc gtcgcctgct ccccctcagc
     6241 atctataaac gctccgccac cttccaggac accttcaccc tcagcctcga agaaggaggc
     6301 atcgtcctgg aaacaaaaaa aggccggcaa cgctggtcat gggaagactt caccgccttc
     6361 aaagagacca tctatttctt ccacgtctac ttcagccccc gctccttctt catgatccca
     6421 aaagactcct tcaaagacat caccgagatc caggccgccc gcaaactgct aaaggaacgc
     6481 atcggaaaat aacttacttc ggcttatacc tcgcctcctc gaaaatggtc cgcaccggca
     6541 tcgtcatcag ccgcgccggc ttcagatcgg tatgcgccgc cgcatacttt ttcacgatct
     6601 gccaacccac ccacgcaccg atattccccg gcgccgcatc gggcatccca agcgtcttgg
     6661 ggccctcacc gatataattc ttgaggatat ccgggtcgat cgtatacaga tcggtaccct
     6721 gcaaaaagaa attccagatc gccgcctcat tacccgcaca ccaatccaat tgcgccttgc
     6781 tataacccgt ccggatactg tccggcacat ccggcaacaa cttgccggcc agccagaaat
     6841 accggccctt catgatcatc tcctcgataa gcggccggtt gtcactgctg tcgggaaaaa
     6901 tatcctgcgc tatcgcattc atacaattcg cctggatata ctccggctcg aaacgccgcg
     6961 agacatactg cggataaagg tcctgcccct tccccgtaag atagaaaggg aaattcctgc
     7021 cggcatagga ctgcaaaccg atagcaaggg aataagtggt gatcgctacg ccgggaccat
     7081 cgaacggacc gacaaaagcc actacctttt gcggcagctt atactccggg aaataatact
     7141 ttacatactt gaaatcctgt tgcagttgat cctccagcca gtccagccgg gggtatttca
     7201 ctttcagcga atcgcttaca ctgagatagc tgaccaggaa ctcccgcgtc gccgcaaagg
     7261 cgaccttgct cgtgtcggtc agcgggccgc cggccccaag gatattcccc acaaaatcgg
     7321 gtgtgaaata cggataatgc cgcgtaagcc gccggagccc ctggacaatg tcattcgaat
     7381 cgagattaaa aaaagcggtg tcgaaccgct cgatatgcac cttcccgaca tcgacccccg
     7441 aaacatccgg cttgtcggca gaaccacagg aaacaaggcc ccagcagaca gcaagaaaga
     7501 acacgtacga tctcatttct tttttttaac gacttttttg gaagccttcg cttcactgtt
     7561 aaaggcaagc gcaagattca cccaatattc aaaatccttc tttttccgga tcccctcctc
     7621 actgacaaag acaaaccccg tcatcgtccg cccgttatgg atcatcggcc ggcaatgatt
     7681 catctccagg gcctcggcat acttatgcgg cccgatccgg caaagcatct catccagccg
     7741 gacacaaaga cacatcttgc ccttgaccat gaaacaaatg ccatggaaca tcttcttttc
     7801 caccacatcg ggaaggtgag caagcgcctc ccttatccgc tgggctaagc gttcattgaa
     7861 agccattccc aaatatagca ctttccccca tcggggcggc atgacccaca ttccgggaat
     7921 tttctttttt ggcgtaactt cgcggtatga agatcgcact ggctcagcaa aattatcata
     7981 tcgggaattt tgaggagaac acccggaaaa taatagcagg catcggccag gccaaagccg
     8041 ccggcgccga tctcgttgta ttcaccgaac tatccgtttg cggttaccca ccccgtgact
     8101 tcctggagtt tgacgatttc atcgctcaat gctacgccgc catcgaccac atccgcgaac
     8161 acgccgatac catcggcgtc atcgtcgggg ctccggtccg caaccccgtc cgcgaaggaa
     8221 aagacctctt caacggggcc tggctgctct acgaaaaaga gatcaaaggc gtcgctcaca
     8281 agacctgcct gcccaactac gacgttttcg acgagtaccg ctacttcgaa cccgcctatg
     8341 aatggaaagt gatcccgttc aaaggcaaaa agatcgctct caccatctgc gaagacatct
     8401 ggaatctcgg cgacaacccc ctctaccggg tctgcccgat ggatcacctg atccgccagc
     8461 accccgatgt catgatcaat atttcagcat cccccttcaa ttacgaccgt gacgaagatc
     8521 gaaaggaggt cgtacgccag aacgtgctga aatacaatct tcccatgtac tactgcaatg
     8581 ccatcggttc acagacagag atcgtcttcg acggcagcag cctcgtctac gatgcaaaag
     8641 gcaacctcgt ccatgaaatg agatccttcg aagaagactt cgccgtggtc agcctgccgc
     8701 acccggtgcc gcccctcttc gaggaactgc ttccgggact ctccgtccgc agcgacgcgg
     8761 atgacgaaga cgccgacatc tccgggcttc ccgacaaccc ggcgaccccg caggctcccg
     8821 ccacgacaac catcgccccc gccgtcttcg acaaggacat gcaggtgtcg aaacagtacg
     8881 atccacaccg catcctcgaa tacctcaccg acgaccgcaa catccgccag atccgcaagg
     8941 ccctcgtcct cggcatccgc gactatttct cgaaaatggg cttttcaaaa gcgatcatcg
     9001 ccagcagcgg cggtatcgac agcgccgtta ccctcgcgct tgcctgcgaa gccctcggaa
     9061 aagataacgt ccgggcgatc ctgctgcctt ccgcctattc caccgcacac tcggtgggcg
     9121 acgcggaaca gttgtcgcgt aacctgggta acccctacga catcatcccc atcagggaag
     9181 tatacgacgc cctcctccat accctggagc cagtatttaa ggatacggcc ttcggcctcg
     9241 ccgaagagaa cctccaaagc cgggcccgcg gcaatctcgt catggccctc gccaataagt
     9301 ttggttatgt aatgctgaac acctcgaata aaagtgaact gtccacgggc tatggaacat
     9361 tatacggcga tatggccggc ggccttagcg tactcggcga cgtatacaag ggacaggtat
     9421 atgccctggc ccgcgatatc aaccgcggcg gagag
//
LOCUS       DATGFG010000465         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_251877, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000465 DATGFG010000000
VERSION     DATGFG010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251877"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2314
                     /locus_tag="VKQ52_08650"
     CDS             <1..2314
                     /locus_tag="VKQ52_08650"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ87296.1"
                     /translation="STDIAMSSGGALWKDLSLGYSILSFATNGIIRRLWGKQYYRSIA
                     FSTVGDSLVYCNTTSGSYEYNLRTGEARTYLPGVAVSRVFRDASGDLWFTTMGAGLYR
                     LISNDVRSIRFTMGHGERVAVTAIARIGHELWIGENHNSIFRLALPGLSQLDRRQYFG
                     YALNRILAIDTTTDGKVLAGGDCALQEAGRDFHSIGGLFTGGVKSMVRIDDHRLLVAM
                     VSGAGIVSLPLHFYSSMTDTLWRERTTVAFYTHDTIYIGTLNGLYRFVKDAPPVFLGE
                     KVPFLRKRVSAIAEAADGTLWVGSYDDAGIIGYKNGRVVAALTRREGLTSDICRCLRV
                     HDNVLWIGTDKGLNRIEPGRTGYLITRYGSRDGLASDIVNVVLVDSPRVYVGTSEGLS
                     FFDEKQPANGEGCRLQLESVMNSGRERIADTGGLVIPWRDKDVRLEFAGISYRSAGDI
                     SYRYRMVGLDSNWRETRENVVEYPDLPSGEYEWQLTAVNKFGKQSRLLRLPITVTIQW
                     WKRPWVVVTTWLLSIGLLSLLLSYRLESIRRGRREKDDLLQRMSDLENIALKSQMNPH
                     FLFNCLNSIQLLISSGDTAGSNAYIAGLARLVRLTLHNSSRTFVPIGDEVDYLSSYLS
                     LEKLRFKEKIDYAIVVDPAIDRMMLSIPPMLIQPYVENALLHGLPPKKGGKGFIRIHL
                     HIAGDQLVVTIEDNGIGRRAAGAQKAGEPTGYPSKGMSLTEDRIGIMNTVYAGDAGIE
                     IIDLEDGAGEPAGTRITLRLPLLRREQAYS"
     gene            2441..>2780
                     /locus_tag="VKQ52_08655"
     CDS             2441..>2780
                     /locus_tag="VKQ52_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87297.1"
                     /translation="MKQSLNLLLVFCLLAHHLNAQEVYTIKSDSVKLTGCDSNELIIE
                     NHTQSVPGFLFNTGRGRTIFKRGAQLLGNTAYLIGGDTVNAWLLGGNSFGQTGVLGTL
                     DNHHLDLYTNG"
BASE COUNT          577 a          725 c          866 g          612 t
ORIGIN      
        1 atcgaccgat atcgccatga gcagcggcgg ggccttgtgg aaggaccttt cgcttgggta
       61 ttctatcctg tcctttgcta cgaacgggat catccgacgt ctctggggca aacagtatta
      121 ccgatcgatc gccttttcca cggtaggtga cagcctggta tactgcaata cgacgagcgg
      181 cagctatgag tataatctcc gtaccggcga agcaagaacg tatttgcccg gcgtcgccgt
      241 gtccagggta ttcagggatg cctccgggga tctatggttc actaccatgg gagccgggct
      301 ctaccggctg atctcgaatg atgtgcggtc gatccggttt acgatggggc atggcgaaag
      361 ggtggctgtc acggcgatcg ccaggatcgg ccatgaacta tggatcgggg aaaatcacaa
      421 ttctattttc cggctggccc tgccggggct tagccagctg gaccgccgac agtatttcgg
      481 atacgccctt aaccggatac tggctatcga tacgacaact gacgggaagg tcctggcagg
      541 cggcgattgt gcactgcagg aagccggccg cgatttccat tcgataggag ggctttttac
      601 cgggggggtc aaatcgatgg tccggatcga cgaccatcgc ctgttagtgg ctatggtatc
      661 gggggcgggg atcgtttccc tgccgctgca tttttattcg tcgatgaccg acacattatg
      721 gcgggagcga accacggtcg ccttttatac gcatgacacc atttacattg gcacgctgaa
      781 cgggttgtac cgctttgtaa aagatgcccc gccggtgttc ctcggcgaaa aggttccctt
      841 tctccggaag cgggtgtccg ccattgccga agctgccgat gggacgcttt gggtcggatc
      901 gtatgacgat gcggggatca tcgggtataa gaacggccgg gtggtggccg cgcttacgcg
      961 gcgggaggga ttgaccagtg atatctgccg atgcctgcga gtgcatgaca atgtcctttg
     1021 gattggaacg gacaagggac tgaaccggat cgaaccgggc aggaccggat atctcattac
     1081 ccgttatggc agcagggatg ggcttgcgtc ggatatcgtg aatgtcgtgc tggtggacag
     1141 cccgcgggtg tatgtcggga cttcggaggg actcagtttc ttcgatgaga agcaaccggc
     1201 gaacggggaa gggtgccggc ttcagctgga atcggtgatg aattcggggc gggagcggat
     1261 cgccgatacg ggcgggcttg tcattccctg gcgggacaag gatgtccggc tggaatttgc
     1321 cggtatctcc taccgatcgg ccggggacat cagctatcgc tatcgtatgg tgggactgga
     1381 cagcaactgg agggaaaccc gggagaacgt cgtagaatac ccggaccttc cgtccgggga
     1441 gtatgaatgg caattgaccg ccgtcaataa gttcggtaag cagagccggc tgttgcggct
     1501 acccatcacg gtgaccatcc agtggtggaa aaggccatgg gttgtcgtca cgacctggct
     1561 gctttcgatc ggcctcctat ccttgttact atcctaccgg ctggaaagca tccgccgcgg
     1621 acgccgggaa aaggacgatc tgctgcaacg gatgagcgac ctggagaaca ttgcattgaa
     1681 gtcccagatg aatccgcatt ttctattcaa ttgtctcaat tccattcagc tgctcatatc
     1741 atccggggat accgccggct ccaatgccta catcgccggg ctggccagac tggtccggct
     1801 gaccttgcac aactcatcgc ggacctttgt tcccatcggg gacgaggtgg attatctttc
     1861 ttcctacctg tcgctggaga agctgcggtt caaggagaag atcgattatg cgatagtggt
     1921 ggacccggcc atcgaccgga tgatgctatc catcccgccc atgttgatcc agccgtatgt
     1981 ggaaaatgcg ctgctacatg gccttccgcc aaaaaaaggc gggaaagggt ttatccgcat
     2041 tcacctgcat atcgcgggtg accagctggt ggtgaccatc gaggacaatg ggatcgggag
     2101 gcgcgcggcc ggcgcccaaa aagctggtga accgaccggg tacccttcga aggggatgtc
     2161 cctgacggag gaccggatcg gcatcatgaa taccgtatat gcgggagatg cgggtatcga
     2221 gatcatcgac ctggaggatg gcgccggcga gccggcgggg acccgtataa cgctccggct
     2281 gccgctattg cgcagggaac aggcgtattc ctgacggatg gctgcgcatg gtgttttcct
     2341 ttgtcacggt tacccatgcc aatgacgcgg ctactgccgc cagcgacacg cccgctcaat
     2401 tgcagcggat gcgttgtttg cgatcgatat tttagggcat atgaaacaat cccttaacct
     2461 gttgctggtg ttctgcctgc tggcccacca tctgaatgca caagaagtat ataccatcaa
     2521 gtcggacagc gtgaagctga ccggttgcga ctccaacgaa cttatcatcg agaaccatac
     2581 gcagagcgtt ccgggcttcc tgttcaacac cggcaggggg cggacgatct tcaaaagagg
     2641 ggcgcagttg ctgggcaata ccgcctatct tatcggcggc gatacggtga atgcctggtt
     2701 gctgggtggc aattcctttg gccaaacggg tgtgttgggg acattggata atcaccacct
     2761 cgacctgtat acgaacggga
//
LOCUS       DATGFG010000466         4545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_708154, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000466 DATGFG010000000
VERSION     DATGFG010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708154"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VKQ52_08660"
     CDS             complement(<1..1188)
                     /locus_tag="VKQ52_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87298.1"
                     /translation="MKPLYALLLILATLSLLLPTTSVRAEGTKQVMPNATNGTGLIVS
                     TTTTFPLGNVGSYLGAPTDDRIYIHISDFTTEMLYYGFNWEPLSPAGTPSTGTYSDVY
                     MNIYNPAGTLVTTIHLPSTAGSNGFISSYINAIQGPIIAGIPTNGYAPLTFTPSMNGD
                     YYVSFYRSSDGGNTHIAGGESMLSKYFDLTVAQGTLKIPGRVHCNEWAFSVYNPSKSD
                     IQDPLTPTNAQFYGYTPDSVTVKVSFPATGFEPLSYIVAFNSFGVKNSGNWLVDRNSI
                     NLPNLVAPYLTGGFDVFLNPPDPTVYPACVIPQAPILLAPVISGCPPGPYNVRFKAPQ
                     AGDYYLLFDLNGVPGFQNNTADRFIELPSQSPGIVTYVWDGKDGLGNVVPANTTFPII
                     FSYR"
     gene            complement(1448..2308)
                     /gene="rfbD"
                     /locus_tag="VKQ52_08665"
     CDS             complement(1448..2308)
                     /gene="rfbD"
                     /locus_tag="VKQ52_08665"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HLZ87299.1"
                     /translation="MEKQTILVTGANGQLGRELQLLAGSFPAFEFVFTDRNQLSIEDP
                     QQVLGFFAEHRPAWCINCAAYTAVDKAETEKETAFRVNGDAPGYLAAACRNTGSRLIH
                     VSTDYVFDGSSATPLKEDNPTGPINSYGASKLEGEHRAMENHPDGTIIIRTAWVYSEF
                     GNNFVKTMIRLMKERPAINVVDDQVGSPTYAADLAAAILHIVSSPRFVPGIYHYSNEG
                     RISWYQFATAIREHIGSACTVNPIPSSQFPTPAKRPHYSLLDKTRIRSTYQVSIPDWE
                     TSLVTCLSRL"
     gene            complement(2332..2892)
                     /gene="rfbC"
                     /locus_tag="VKQ52_08670"
     CDS             complement(2332..2892)
                     /gene="rfbC"
                     /locus_tag="VKQ52_08670"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HLZ87300.1"
                     /translation="MPFKTTDIQGLLIFEPSVFKDERGYFFESYNEQTFRKQGLDMRF
                     VQDNQSFSYHGVIRGLHYQLDPYAQTKLVRVLEGRVIDVAVDIRKGSPTFGKHFAIEL
                     SAENKLQLFIPRGFAHGFSVLSETAQLSYKCDGLYSKESEGGIRFDDPQLNIDWQVPA
                     AKAIVSAKDLELPVFAECRNTFQYKG"
     gene            complement(2897..3961)
                     /gene="rfbB"
                     /locus_tag="VKQ52_08675"
     CDS             complement(2897..3961)
                     /gene="rfbB"
                     /locus_tag="VKQ52_08675"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HLZ87301.1"
                     /translation="MVNYRKTILITGGAGFIGSHVVRLFVTKYPQYRIVNGDALTYAG
                     NLANLKDIQSAPNYIFEKADITDDARIKQLFDQYKFDAVIHLAAESHVDRSIMDPLAF
                     VRTNVLGTANLLNIAGAAWKNDFKDKLFYHVSTDEVYGSLGAEGFFTEETPYDPRSPY
                     SASKASSDHFVMAWHHTYGMPVILSNCSNNYGSNHFPEKLIPLMINNILNNKPLPVYG
                     KGENVRDWLFVDDHARAIDTIFHNGRVGEKYNIGGFNEWKNIDIVNLLCGIMDRKLGR
                     APGESAKLITYVKDRPGHDLRYAIDATKLNKELGWKPSLQFEEGLEKTVDWYLSHQDW
                     VKEVTSGDYQKFYNEQYSKR"
     gene            complement(3961..>4545)
                     /locus_tag="VKQ52_08680"
     CDS             complement(3961..>4545)
                     /locus_tag="VKQ52_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HLZ87302.1"
                     /translation="RMGINTYDVIEAAGTKWNFLKFQPGLVGGHCIGIDPYYLTYKAA
                     ALGYESKVITSSRYINDDMARYVARKIITHVLRISNEPRVLVKGVTFKENVSDIRNSR
                     IVDTVKELLAFNITVDVEDPYAMEEEVHEEYGLHLARKPAGDYDAVIITVPHVQYRDL
                     DDAYFTSITREGALIADLKGTYKNKITNRKYWSL"
BASE COUNT          927 a         1038 c         1477 g         1103 t
ORIGIN      
        1 ccggtaagag aagatgatgg ggaaggtggt attcgccggt acgacattgc cgagaccatc
       61 cttgccgtcc catacatagg tgacgatgcc cggtgactgg ctcgggagtt ctatgaaccg
      121 gtcggcggtg ttgttctgga atccggggac gccgttgaga tcgaagagca ggtaatagtc
      181 ccctgcctgt ggcgccttga accggacatt atagggtccc ggcggacagc ccgagatgac
      241 gggcgccagc aggatgggcg cctggggaat gacgcaggcc gggtaaacgg tggggtcggg
      301 tgggttcagg aatacgtcga agcccccggt gagataggga gccacgaggt tgggcaagtt
      361 gatcgaattg cgatcgacca gccagttgcc gctgttcttt acaccgaagc tgttgaaggc
      421 gacgatatag gacagaggct cgaaaccggt ggcagggaag gagaccttca ccgtgacgga
      481 gtcgggtgta tagccataga actgggcgtt ggtaggggta agcggatcct ggatatcact
      541 cttggaagga ttatataccg agaaggccca ctcgttacag tggacgcggc cggggatctt
      601 cagcgtaccc tgcgcgaccg tgaggtcgaa gtatttggac agcatcgatt ccccgccggc
      661 gatatgggta ttgccgccgt cgctgctccg atagaaagaa acataatagt ctccattcat
      721 cgaaggggtg aaagtaaggg gggcgtagcc gttggtcggg atcccggcga tgatcggtcc
      781 ctgtatggcg ttgatatagc tgctgataaa accattggaa cccgcggtgg aaggaaggtg
      841 gatggtggtc accagggtac ccgcggggtt gtagatattc atgtagacgt cggaataggt
      901 cccggtgctg ggcgtccctg cgggagagag aggttcccag ttgaacccat aatacagcat
      961 ttccgttgta aagtcactta tatgtatata tatccggtcg tcggtagggg ccccgagata
     1021 gctgcccaca ttaccaagag ggaaggtggt cgtggtcgaa acgatcagac ccgtaccgtt
     1081 tgtagcattg ggcattacct gttttgtgcc ttcggcgcgg accgaagtgg tcggcagcag
     1141 aagggacagg gttgccagga taaggagtag ggcgtataaa ggtttcataa ggatggatta
     1201 aggttgaccg atcaccggga tctgaccccg gggggttaat cttctggccg caagctaggc
     1261 aacaaaccca ttccctacaa tcgtgaaaag tacgcgtgta gaataggaaa atacgttctg
     1321 cgtttgccca ggttggagca gagaggcata cgcatacgat taatccaaca tctttgagcc
     1381 tgctatacgg aataagtttt ccacattaat agaaatactt atttactgtt agataaatga
     1441 ctgtcagtta aagacgggag agacaggtga ccaggctcgt ttcccagtcg gggatggaaa
     1501 cctggtatgt gctgcggata cgcgttttgt ccaacaggga ataatgcggg cgttttgcgg
     1561 gggttgggaa ctggctgctg gggatcgggt tgaccgtgca ggcgctgccg atatgctcgc
     1621 ggatcgcggt ggcaaactgg taccagctta tccggccttc gttgctataa tggtagatcc
     1681 cggggacgaa gcgggggctg cttacgatat ggaggatggc ggcggcgagg tcggcggcat
     1741 aggtggggga gccgacctgg tcatctacta cgtttatggc gggccgttcc ttcatcaggc
     1801 ggatcatggt ctttacgaag ttgttgccga attcggagta gacccaggcg gtgcggatga
     1861 taatggtgcc gtcggggtgg ttttccatgg ccctgtgttc gccttcgagt ttggaggcgc
     1921 catagctgtt tatgggcccc gtgggattgt cttcctttag cggagtggcg gaactgccgt
     1981 cgaagacgta gtcggtggag acgtggatga ggcggctacc ggtgttgcgg caggcggcgg
     2041 cgagatagcc gggggcgtca ccgtttaccc ggaaggcggt ttccttctcc gtctcggctt
     2101 tatccacggc cgtataggcg gcgcagttga tgcaccaggc cgggcgatgt tcggcaaaga
     2161 atccgagcac ctgctgcggg tcttcgatgg aaagttggtt ccgatcggtg aaaacgaatt
     2221 cgaaggccgg gaaggatccc gccagcagtt gcagttcgcg gccgagttgg ccgttggcgc
     2281 cggtgacgag gatggtttgc ttttccatgg agctggtgag ccttttggtg gttagccttt
     2341 atattgaaag gtattgcggc attcggcgaa aacgggcagc tccaggtcct tcgcggagac
     2401 gatggctttt gctgccggga cctgccagtc gatgttcagc tgcgggtcgt cgaagcggat
     2461 gccgccttcg ctttccttgc tatagaggcc gtcgcattta taagatagct gggccgtttc
     2521 gctgagtacg gaaaaaccgt gggcgaagcc gcgggggatg aacaattgca atttattctc
     2581 cgcggagagt tcgatggcga aatgtttccc gaaggtgggc gagcccttgc ggatgtctac
     2641 ggccacgtcg atgacccttc cttccagaac acgcacgagt tttgtctggg cataggggtc
     2701 caactgatag tgcaggccac ggatcacgcc atgataggaa aaggattgat tgtcctggac
     2761 gaatcgcatg tcaagcccct gtttccgaaa ggtttgttcg ttataggact cgaagaaata
     2821 cccccgttcg tctttgaata cggagggttc gaagatgagc agtccctgga tgtcggttgt
     2881 tttgaaaggc atccttttat cttttgctgt attgttcgtt gtaaaatttc tgatagtcgc
     2941 cggaggtcac ctcttttacc cagtcctggt gagagagata ccagtcgacg gttttttcca
     3001 ggccttcctc gaactggagc gagggtttcc agcccagttc cttgttgagc tttgtcgcat
     3061 cgatggcata gcggaggtca tggccggggc ggtcttttac gtaggtgatg agttttgcgg
     3121 actcgcccgg ggcgcggccg agcttcctgt ccatgatgcc gcagagcagg ttgacgatgt
     3181 cgatgttctt ccattcgttg aagccgccga tattgtattt ctcccctacc ctgccattat
     3241 ggaagatggt atctatcgcc cgggcgtggt catcgacgaa aagccagtcg cggacgttct
     3301 cgcccttgcc gtagacggga aggggtttat tattcaggat gttgttgatc atcaggggga
     3361 tcagcttctc cgggaagtgg ttgctgccat agttgttgga gcagttcgag aggatgacgg
     3421 gcatgccata cgtgtgatgc caggccatga cgaaatggtc ggaagaggcc tttgatgcgc
     3481 tatagggaga gcgggggtcg tagggtgtct cttcggtgaa gaacccttcg gcgccgaggg
     3541 agccgtagac ctcgtcggtg gagacatgat agaacagctt gtctttgaag tcgttcttcc
     3601 atgcggcccc ggcgatattg agcaggttgg cggtgccgag gacgttggtg cggacgaagg
     3661 ccaggggatc catgatggag cgatcgacat ggctttcggc cgcaagatgg ataacggcgt
     3721 cgaatttgta ttgatcgaag agctgtttta tccgcgcgtc gtcggtgatg tcggcctttt
     3781 cgaagatata gttgggcgcc gactgaatgt ctttaaggtt cgcgaggtta cccgcatagg
     3841 taagggcgtc gccattgacg atcctgtact gcggatattt tgtcacgaat aatcttacta
     3901 catgcgatcc gatgaagccg gctccgccgg tgatcaggat agtcttgcga taatttacca
     3961 ttacagactc cagtattttc tgttagtgat cttgttcttg taggttcctt tcaggtcagc
     4021 gatcagggcg ccttcgcggg tgatggaagt aaagtaagca tcgtcgagat ccctgtattg
     4081 gacatggggc acggtgatga tgacggcatc gtagtcgcct gccggtttgc gggcgaggtg
     4141 gagcccgtat tcttcatgca cttcttcctc cattgcgtaa gggtcttcga catcgaccgt
     4201 gatgttaaag gccagcagct cctttaccgt gtcgacgatg cgggagttgc ggatatcgct
     4261 gacgttctcc ttgaaggtga cgccttttac gaggacgcgg ggttcgttgg agatgcggag
     4321 gacatgggtg atgatcttcc ttgcgacata gcgggccatg tcgtcgttga tatagcggga
     4381 ggaggtgatc actttggatt cgtagcccag ggcggctgcc ttataggtga ggtagtaggg
     4441 atcgatgccg atgcagtgtc cacctacgag gccgggctgg aatttcagga agttccactt
     4501 tgttcccgcc gcctcgatga catcataggt gttgatcccc atgcg
//
LOCUS       DATGFG010000467         4755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1487408, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000467 DATGFG010000000
VERSION     DATGFG010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487408"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /gene="rsmH"
                     /locus_tag="VKQ52_08685"
     CDS             <1..558
                     /gene="rsmH"
                     /locus_tag="VKQ52_08685"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HLZ87303.1"
                     /translation="GDMDMRMDQRQTLTAYDVVNTWSEQQLHKLFEQYGEVTNAKTLA
                     RTIVQVRNTMSLKTIANFKTALNSVVKGNPNKYFAQVFQALRIEVNDELGALKEMLQQ
                     VPGLLSTGGRVAIITFHSLEDRIVKNFFKRGTFEDEDPADPFGQSTAPPLLKVITKKP
                     VIPGDAELRQNPRSRSAKLRVAQRI"
     gene            591..1016
                     /locus_tag="VKQ52_08690"
     CDS             591..1016
                     /locus_tag="VKQ52_08690"
                     /inference="COORDINATES: protein motif:HMM:NF039650.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HLZ87304.1"
                     /translation="MSKKAQNNETTPPWEDTPGAGQAAQPEPASKPATSKPTSRPRWK
                     PSLPGYRWIMKNIGFFLFLAALAVVYIYNGHYADKISRDITRTNKELKELQYEYKTLK
                     SEVMFRSKQSELARAVEPFGLKELVRPPFVLTDSIEQQH"
     gene            1054..3084
                     /locus_tag="VKQ52_08695"
     CDS             1054..3084
                     /locus_tag="VKQ52_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HLZ87305.1"
                     /translation="MEVKRDILWRVYLCFLGIAALSLVVLGKVFYIQRVQGAYWKGLS
                     DSLHQKFIELDAERGTIYSEDSSMLSTSIPFFNIYIDFGADGLREKNGRRFFDNLDSL
                     SGCLAGLFGDQSAAAYKKLLLKGYRTTDRYYELQSNVSFEKYQKLRDFPLVRQGRNKS
                     GFIAEVVNKRLNPFGLLANRTIGLARANAQNVGLERTYDTLLKGETGRRLVRYIAGGT
                     YIPVEGYEIEAENGRDIVTTIDVNIQDIAENALQKELIENEAEHGTCIVMEVATGKIK
                     AIANLGRQPDGSYWEDLNYAIRATEPGSTFKLATLLSLLEDKKVSLNERLNLEGGRWK
                     IAGRTVYDSEPHDNSLFTVKQAFEISSNVGMAKLAVAHYSGNPGQFIEHLRKLRLDHP
                     TGIDLAGESTPVLITPRSRTWSATALPWMAFGYEVLVTPLQTLTLYNAVANNGRMMKP
                     YLVSAVQESGITVKEAQPIAIVEKICSDETLRQLKECLNGVCQDPEGTGFKVFKDAFY
                     KVAGKTGTALVANRSRGYADHIYQSTFVGYFPAAAPKYTCIVVIKNKPFAKKYYGAAV
                     AAPVFREVADHLMSASVDQGLNYADSAGVADHNRLFASTAAPASQPAQNPSPSSRQTV
                     PDVKGMGLKDALYLLEGMDMRVAVRGSGKVRTQSIEPGSPLQKKETIFIQLD"
     gene            3104..4570
                     /locus_tag="VKQ52_08700"
     CDS             3104..4570
                     /locus_tag="VKQ52_08700"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HLZ87306.1"
                     /translation="MANLQEIVYKVSIRTIVGPRDRAIANLQTDSRLAGPGTLFIALK
                     GTHTDGHQYIPAVIAAGAVAIVCEDLPAGLQEDITYIQVEDSAASAGIIAHNFYGQPS
                     EKLKLVGVTGTNGKTTIATLLYKLYSELGYKCGLLSTVQNHIGDRIVPATHTTPDALH
                     LNALLKEMLDENCTHVFMETSSHAIHQHRIAGLRYAGGIFSNITHDHLDYHKTFEEYI
                     RVKKSFFDSLPSEAFAISNADDKRGAVMLQNTNAKKYFYSLRTLAEFKGRILENNLTG
                     LVMTINDQEVHFRLIGEFNAYNLLAVYGAAICLGEERSEILRVLSTLSGAEGRFDYVV
                     SPKEKIIGIVDYAHTPDALINVLATIQKLRKGHEKIITVVGAGGDRDKTKRPLMGAAA
                     AEYSDRVIITSDNPRSEEPEQIIRDIEAGLNTAAKRKYISITDRREAIRTALSLAGPE
                     DILLVAGKGHEKYQEIKGVKHPFDDKQILLEFFELLDK"
     gene            4576..>4755
                     /locus_tag="VKQ52_08705"
     CDS             4576..>4755
                     /locus_tag="VKQ52_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87307.1"
                     /translation="MLYTLFEWFRQHGIKVPGGALFQFITSRVLLAVVLSLVISMIFG
                     KRLIRLLVKKQIGETV"
BASE COUNT         1225 a         1403 c         1251 g          876 t
ORIGIN      
        1 ggcgatatgg acatgcggat ggatcagcgc cagacgctca cagcatatga tgtcgtcaac
       61 acctggtcgg aacagcaact gcataagctg ttcgagcagt atggcgaggt aaccaatgcc
      121 aaaacgctgg cccgaaccat cgttcaggta cgcaacacaa tgtcgctgaa gaccatcgcc
      181 aatttcaaaa cggcgctgaa tagcgtggtg aaagggaatc ccaataaata ctttgcccag
      241 gtgtttcagg ccttacgcat tgaagtgaac gatgaattgg gagctttaaa agaaatgttg
      301 caacaggtgc ccggcctcct ctctacaggc ggtcgtgtag cgatcatcac ctttcattcc
      361 ctggaagaca ggatcgtcaa gaacttcttt aaaagaggaa ccttcgaaga cgaagaccct
      421 gctgatccct tcggacaaag cacggcacct cccctgttaa aagtcattac taaaaaaccg
      481 gtgatccccg gcgacgccga attacgccag aacccccgat cccgcagcgc caagctacgg
      541 gtcgctcaac gcatatagat aagaagaaca cagaggcaga agaaggagac atgagtaaga
      601 aagcacaaaa taacgaaacg actcccccat gggaggatac accgggcgcc ggacaggcgg
      661 cccaacccga acccgcatca aagcctgcca cctccaaacc cacaagccgc ccccgctgga
      721 aaccctccct gcccggctat cgctggatca tgaagaatat cggcttcttc cttttcctcg
      781 ccgcactggc tgtggtctac atctataacg gccattatgc cgacaagatc tcccgggata
      841 tcacccggac aaacaaagaa ctgaaagagt tgcaatatga atacaaaacg ctgaaaagcg
      901 aagtgatgtt ccggagcaaa cagagcgaac tcgccagggc cgtggaaccc tttggactaa
      961 aggaactggt acggccgccc tttgtgctca cggacagcat tgaacaacaa cactaaaaaa
     1021 tatctcccgg ctgcaaagag ccggggcgcc agcatggaag tcaaacgcga tatattatgg
     1081 agggtatacc tctgctttct cggcatcgca gcgctatcgc tggtggtgct ggggaaggtg
     1141 ttttatatcc aacgcgtaca gggcgcctat tggaaaggac ttagcgacag ccttcatcag
     1201 aaattcatcg agctcgacgc cgaacgggga acgatctaca gcgaggacag cagcatgctc
     1261 agcacctcga tccccttttt caatatctat atcgactttg gcgccgacgg cctccgggaa
     1321 aagaacggcc gccgattctt cgataacctc gactcccttt ccggctgcct tgccggcctc
     1381 tttggcgacc agtccgctgc cgcctacaaa aagctgctgc tgaaaggcta tcgcaccacc
     1441 gatcgctact acgaactgca atccaatgta tcctttgaaa aataccagaa actccgcgac
     1501 ttccccctcg tccgccaggg tcgcaacaaa agcggcttca tagcggaagt ggtcaataaa
     1561 agattgaatc cgttcggctt gctggccaat cgtaccattg gcctggcaag agcgaatgcc
     1621 cagaacgtcg gactcgaacg cacctatgac accctcctca agggggagac cggccgccgc
     1681 ctggtccgct atatcgccgg cggaacctat atccccgtgg aaggctatga gatcgaggcg
     1741 gagaacggca gggatatcgt aacgaccatc gacgtgaaca tccaggacat cgccgagaac
     1801 gcgctgcaaa aagagctgat cgaaaacgag gccgagcatg gtacctgcat cgtcatggaa
     1861 gtagccaccg gtaagatcaa ggccatcgcc aaccttggcc gccagcccga tggctcctac
     1921 tgggaagacc tgaactatgc catccgggcc acggaaccgg gctccacgtt caaactggcg
     1981 accctgttat ccctgctgga agataaaaag gtctccctca acgagcggct caacctggag
     2041 ggtggccgct ggaagatcgc cggccgcacc gtctatgact cggagccaca cgataacagc
     2101 ctctttaccg taaagcaggc attcgagatc agctccaatg tcggcatggc aaaactcgcc
     2161 gtcgcccatt attccggcaa tcccggtcag ttcatcgagc atctccggaa gctgcgcctg
     2221 gatcatccca cggggatcga cctcgcgggg gaatcgacac cggtgctcat cacaccgaga
     2281 tcccgcacgt ggagcgccac tgccctgccc tggatggcct ttggctatga agtgctggtc
     2341 acccctcttc agacccttac cctctataac gccgtcgcca acaacggccg gatgatgaag
     2401 ccttacctcg tgagcgccgt acaggaatcg gggatcacgg taaaagaggc ccagcccatc
     2461 gccatcgtag agaagatctg cagcgacgag acccttcgtc aactgaaaga atgtctcaac
     2521 ggcgtatgcc aggaccccga aggaacgggc ttcaaagtat tcaaggatgc tttttacaag
     2581 gtagccggca agacgggcac cgctctcgtg gccaaccgct cgcgcggcta tgccgaccat
     2641 atctatcaat ccacctttgt cggttatttc ccggccgcgg ccccgaaata cacctgcatc
     2701 gtggtgatca agaacaaacc ctttgcaaag aaatactatg gcgcggcggt agcggcgccg
     2761 gtattccggg aagtggccga tcatttgatg agcgccagcg tcgaccaggg gctcaattat
     2821 gccgactcgg cgggcgtcgc cgaccacaac cgcctgttcg cgtcgacggc ggcaccggca
     2881 agccaaccgg cacaaaaccc ttcgccctcc tcccgtcaaa cagtacccga tgtaaagggt
     2941 atggggctga aggacgccct ctacctgctg gaaggcatgg acatgcgggt ggcggtaagg
     3001 ggcagtggaa aggtaagaac acaaagtatc gagccgggca gccccctgca aaaaaaggaa
     3061 accatattta ttcaactgga ttaaaacaga acatgccgac gtgatggcaa acctgcagga
     3121 aatagtatat aaggtaagta tccggaccat cgtagggccc cgggacaggg ctatcgccaa
     3181 cctgcaaacg gattcccggc tggcgggacc cggtaccctg ttcatcgccc tgaagggcac
     3241 ccacaccgac gggcatcagt acatccccgc cgtgatcgcc gccggcgccg tcgccatcgt
     3301 ctgcgaggac ctgccggccg gtctgcaaga ggacatcacc tatatccagg tggaagacag
     3361 cgccgcctcc gccggcatca tcgcgcataa cttctatggc cagccttcgg agaaactgaa
     3421 gctggtgggc gtgaccggca caaatgggaa gaccaccatc gccaccttat tatacaagct
     3481 ctattccgaa ctggggtata aatgcggcct cctcagcacc gtgcagaacc atatcggcga
     3541 ccggatcgtt ccggccacgc ataccactcc cgacgcgttg catctcaacg ccctgctaaa
     3601 ggaaatgctg gatgagaact gtacccatgt ctttatggag accagctccc atgccatcca
     3661 tcagcaccgg atcgccgggc ttcgttatgc cggggggatc ttcagcaata tcacacacga
     3721 ccacctcgac tatcacaaaa ccttcgagga atatatccgt gtaaagaaat ccttcttcga
     3781 ctccctgccc tccgaggcct ttgccatctc gaacgccgat gacaagcgtg gcgccgtgat
     3841 gctccagaac accaacgcaa agaaatattt ctatagcctc aggaccctcg ccgagttcaa
     3901 gggacggata ctggagaaca acctcaccgg gctggtgatg accatcaacg accaggaggt
     3961 ccatttccgg ttgatcgggg agttcaacgc ctataacctg ctggccgtgt atggcgccgc
     4021 catctgcctg ggtgaagaaa ggagcgagat cctgcgtgtg ctcagtacgc tcagcggggc
     4081 ggaaggccgg ttcgattatg tcgtatcgcc aaaggaaaag atcatcggca tcgtcgatta
     4141 tgcccataca ccggatgcgc tgatcaatgt cctggctacg atccagaagc tcaggaaggg
     4201 acatgaaaag atcatcaccg tcgtgggggc cggcggcgat cgcgacaaga caaaacgccc
     4261 gctcatgggc gctgccgccg cggaatacag cgacagggtg atcatcacct ccgacaaccc
     4321 ccggagtgaa gaaccggagc agatcatccg cgacatcgag gcgggactga acaccgctgc
     4381 aaaacggaaa tatatttcga tcaccgaccg gagagaggcc atccgcaccg cgcttagcct
     4441 cgccggaccg gaagacatcc tgcttgtcgc gggaaaagga cacgagaaat accaggagat
     4501 aaagggagtg aaacacccct ttgacgacaa acagatctta ttggaatttt ttgaattgct
     4561 ggataaataa gaagtatgct gtatacttta tttgaatggt ttagacaaca cgggataaag
     4621 gtgcccggcg gcgccctgtt ccagttcatc acctcccggg tgctgctggc cgtggtattg
     4681 tcactggtca tcagcatgat ctttggcaag cgactgatcc gcctcctggt aaagaagcag
     4741 atcggggaga ccgta
//
LOCUS       DATGFG010000468         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1929, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000468 DATGFG010000000
VERSION     DATGFG010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2930)
                     /locus_tag="VKQ52_08710"
     CDS             complement(<1..>2930)
                     /locus_tag="VKQ52_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLZ87308.1"
                     /translation="AMTGRMVVGGEALQWGQIQELTERGWDMEIINEYGPTEATVGCC
                     VYRLRTIGNENGRGAGVPIGRPMGNVRIYIVDKTGGLSPVGVAGELWIGGQQLARGYW
                     NRSELTAEKFIRDPFSQDPEARVYRTGDKGRWLRDGNIEYLGRMDEQVKIRGYRVEPG
                     EIEGMLEQSGLVRQTAVVAREDGAGGKRLVGYVVGKEGYHREALIGWLRDRLPEYMIP
                     VQWMELEAMPLTVNGKIDRRALPETEAVEVMASYVAPRTACEQTLVEIWKETLELEEL
                     GITENFFTAGGDSIKAIVAVSRIRSALSTELQLADLFEHTTVLKLAAFLDKNHVDREY
                     AQLSRKISEQVEVLRQKTMQRLAHNQQIEEVYPMSDIQKGMLFFSSLNPGLPVYHDQF
                     AHEIVLMDFEPELFARAFQLLVDKHSILRTIFDFEEALQLVLKKIPLEIGYIDLRSKE
                     PDEQRRVIHDHMVKERSKAFNYWASPLWRASLFRTHQDAVVFLFQFHHAILDGWSRAS
                     FETELHNLYISLKRGVDHKPGALRSTNKDAVIGELIEKCNEENLRFWENEMADHRRVD
                     IFSSVPTAQNFNKRYDADLAKRIKAVAKENDMPVKNFLFAAVWYTIKLLTYSEDVTVG
                     LVTHNRPAVEDGEKILGCFLNTVPVRISLNGERGLSWRAYFQKVKQKLEEIKKRERTT
                     LLDISNRLGEKPKNGNPFFDIIFNYTDFYIYKELYDESSGSDRRREPGNFFSFELTNT
                     YLDIGANLTDNGLFVHYILTRELKSGISLEQFHGYFERVLDRALYFSEELIMHDRLLS
                     VAATQNLFRLSHGGHIFYPPEETMVSLFEEQVRRTPGALSVVFKEQALTYRELDEWSN
                     RVGHALYRKGAGPEVLVPICVERSLEMVVGMLAILKTGGAYVPIDPAYPVERIGYMLA
                     DTGAKLVVSSRACRGLLGEGVEVVLLDGEDHLSELSCAIGGVARSEQLAYVIYTSGST
                     GRPKG"
BASE COUNT          669 a          948 c          562 g          751 t
ORIGIN      
        1 ccctttggcc tgcccgtcga tcccgatgtg tagatcacat acgcaagctg ctccgaacgc
       61 gcgacgccac ctattgcgca cgacaactcg gacaggtgat cctccccatc cagtaggact
      121 acttccactc cctcgcccag caaaccccgg caggcacgac tgctcactac cagcttcgcc
      181 cccgtatcag ccagcatata cccaatacgc tccaccgggt atgccgggtc tatcggcaca
      241 tatgcccctc ccgtcttcag tatcgccagc atccccacca ccatctcaag gcttctttcc
      301 acgcagatcg gcaccagtac ctccggaccc gcgcccttgc gatacagcgc atggcctaca
      361 cggttcgacc attcatccag ctcccggtac gttaatgctt gctctttgaa caccaccgac
      421 aatgcccctg gcgttcgccg cacctgctct tcgaacaacg acaccatcgt ctcctctggt
      481 ggataaaaga tgtgcccccc atgcgatagc ctgaataaat tctgcgtagc cgcaacggaa
      541 agtaaacgat cgtgcatgat caattcttcg gaaaaatata gagcccggtc cagtacccgc
      601 tcgaaatagc catgaaattg ttcaagagag ataccagatt tcagttccct tgtcaaaata
      661 tagtgaacga acagcccgtt gtcggtaaga ttcgcgccaa tatccaaata agtattggtc
      721 agctcaaatg aaaagaaatt accgggttca cgccttctgt cgcttcctga gctttcatca
      781 tacagctcct tgtaaatata aaaatcagtg taattgaaaa tgatatcaaa aaacgggttg
      841 ccgtttttcg gcttctcacc aagcctgttg gagatatcca gcaaggtcgt tctttcccgc
      901 tttttaatct cttcaagctt ctgcttaact ttttgaaaat acgcccgcca gctaagaccc
      961 ctttctccat tcagtgagat cctcacgggg acggtattca gaaaacagcc taatatcttt
     1021 tcgccatctt ccacggccgg cctgttatgc gttacgagcc ccaccgtgac atcctccgaa
     1081 taggtcaaca actttattgt ataccaaacc gccgcaaaca aaaagttctt gacgggcata
     1141 tcgttctctt tcgcaactgc ctttattctt ttcgcaagat ccgcatcgta tcttttattg
     1201 aagttctggg ctgtgggcac tgacgaaaaa atatccaccc gtctatgatc cgccatttca
     1261 ttttcccaga accgcaggtt ctcctcgtta cacttttcga tcaactctcc gatcacagcg
     1321 tctttattgg tggatcttaa tgcccccggc ttatgatcga cccctctttt taaggatata
     1381 tataagttat ggagctccgt ctcgaaagat gccctgctcc atccgtccag tatagcatga
     1441 tgaaattgaa aaagaaatac aacggcatcc tggtgggtcc tgaacaagct tgcccgccat
     1501 aacgggcttg cccaatagtt aaaggccttc gaacgctcct tcaccatatg atcgtgaata
     1561 actctccgct gttcgtcagg ctccttcgac cgcaggtcta tataccctat ctccaaaggt
     1621 atctttttaa gcaccagctg cagcgcttct tcgaaatcaa atattgttct gaggatagaa
     1681 tgcttatcga ccaaaagttg gaatgccctt gcaaataact ccggctcgaa atccatcagc
     1741 acgatctcgt gggcgaactg atcatgatat accggcaatc cgggattcag ggaagaaaag
     1801 aacaacatac ctttttgtat gtcgctcatt ggatatactt cttctatttg ttggttatgg
     1861 gcaagacgct gcattgtctt ctgcctcaat acttccacct gctccgatat cttcctgcta
     1921 agctgggcat attctctgtc aacgtggttc ttatcgagga aagcggccag ttttaacacg
     1981 gtagtgtgct caaagagatc cgccaattgc aattccgtcg ataatgccga tcggatcctg
     2041 cttaccgcta ctatggcctt gatagaatcg ccaccggccg taaagaagtt ctcggtaatg
     2101 cccaattcct ccagctccaa cgtttctttc catatctcca ccaatgtctg ctcacatgcc
     2161 gtacgcggcg ctacatagga ggccataact tccactgcct ccgtctccgg cagcgcccgc
     2221 cggtcgatct ttccgttgac cgtcaacggc atcgcctcca gttccatcca ctgcaccggg
     2281 atcatgtatt ccggcaaccg gtccctcaac catcctatca gcgcctcccg gtggtacccc
     2341 tccttcccta caacatatcc caccagtctc ttgccccctg cgccatcctc ccgcgccacg
     2401 actgccgtct gtcgcaccag cccgctctgc tcaagcatac cctctatctc tccgggttcc
     2461 accctgtagc cgcggatctt cacctgctcg tccatccggc ccaggtattc gatatttcca
     2521 tccctcagcc aacgaccctt atctcctgtc cggtagaccc gtgcttccgg atcctgactg
     2581 aatggatcac ggatgaactt ctccgccgtc agctccgaac ggttccaata cccccgtgcc
     2641 aactgttgcc cgcctatcca taactctccc gcaaccccca ccggcgacaa tcctcctgtc
     2701 ttatctacga tgtagatgcg tacattcccc atcggccgcc ctataggcac ccctgcccct
     2761 ctcccgtttt catttcctat cgtcctcaag cgatatacac aacagcccac cgtcgcctcc
     2821 gtcgggccgt attcattgat gatctccata tcccaacctc tttccgtcaa ctcctggatc
     2881 tgaccccatt gaagggcctc tccccccact accatcctcc cagtcattgc
//
LOCUS       DATGFG010000469         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_345583, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000469 DATGFG010000000
VERSION     DATGFG010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345583"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VKQ52_08715"
     CDS             <1..678
                     /locus_tag="VKQ52_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ87309.1"
                     /translation="SIASHELKTPLTTIKAYVQLLERSIGEEEPGKLYVERALAQIRK
                     LDNLIVDLLDLSKIESGKLKFNKKLFSFETTLSNAIEMIRQTYSDYRITRTGEADIEL
                     YGDEMRIEQVIINYLTNAVKYSPDRKEIDVEASVRPDGQLYVQVRDYGIGIRKDHQAN
                     IFSKFYRVEESASRFQGLGIGLYICAEIIRRHEGEYGVESEPGQGSAFYFSVPLNPEA
                     ALRKVAG"
     gene            716..>2997
                     /locus_tag="VKQ52_08720"
     CDS             716..>2997
                     /locus_tag="VKQ52_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87310.1"
                     /translation="MMRPGLLRNLQIGFGLSLLILVITSVASYSSIQNLMDGAGRVDH
                     TDSVLIKIDRIVAVLRDAESGQRGYLLTGDESFLAGYEGAQDRMKALVDSIGEMTAEN
                     PREEKNIVELRQVLFLPLTTLRVMIDQKKTDNIYNTTALMLAREQMQDARKVLQRMED
                     EERRLMSSRIDKVRRYSAYTPVLILTAALLSILITIFFYRRVHQDYLERAALYAELQQ
                     KDAEVARRIDIISEIADQISDGHYQVRVSDDMKGSLGELSGSLNKMAESLDYSFGLLS
                     DKEWLQSGIAKLSDEMVGETDLQALVMHVIAFIAAYSHSRVGAFYILDPVAGVLTRAG
                     AFALSGGDDRVKVKLGEGLVGQVARDGKMVVLNDIRLGEWIIRLASGDITPRTVLAFP
                     IFHEKKVRGVIELGSLEGYAERDKEFFTSIGDNIGAAIQSIESRQRLQELLEETQAQA
                     EELQSQHTELEHLNLELEVQAEKLQVSEEELKVQQEELMQTNQELEERSRTLEEKNQL
                     ILIRNLDIQRKAEELALTTKYKSEFMANMSHELRTPLNSILLLSRLLSENGGGNLSDE
                     QIEYARVIQNSGQGLLQLIDEILDLSRIESGKLPLEHALVTVTDVIDDMRMLFEPIAK
                     DKNLDLHFVIEEGTPRQLETDKMRLEQIVKNLLANAFKFTHKGSVTLRVRVSAEQKNF
                     LEFVVRDSGIGIPEDKHRLIFEAFQQADGSTRRLYGGTGLGLSISRELARLLGGDIRV
                     SSRPDEGAEFTVSIPQFKMEP"
BASE COUNT          666 a          717 c          992 g          622 t
ORIGIN      
        1 agtatcgcga gtcatgagct gaagaccccg ctgaccacca tcaaggccta tgtgcagctg
       61 cttgaacgca gtatcgggga ggaagaaccg ggtaaattgt atgtcgagcg ggcgcttgcc
      121 cagatacgaa agctggacaa cctgatcgtc gacctgctcg atctgtccaa gatcgagagc
      181 gggaagctga aattcaataa gaaattattc tcctttgaga ctactttgtc taatgcgatc
      241 gagatgatcc gtcagaccta ttccgactac cgtattacgc ggacggggga ggccgatatc
      301 gagctgtatg gggatgagat gcggatcgaa caggtgatca tcaattacct gaccaatgcc
      361 gtgaagtatt cgcccgatcg gaaggagatc gatgtcgagg cgagcgtgcg gccggacggg
      421 cagctgtatg tgcaggtgcg ggactatggg atcggcatcc gcaaagacca tcaggccaat
      481 atcttcagca agttctaccg cgtggaggaa tcggccagcc gcttccaggg actggggatc
      541 ggactatata tctgtgcgga gatcattcgc cggcatgaag gagagtatgg agtggagagc
      601 gaaccggggc aagggtcggc gttctacttt tccgttccgt taaatcctga agccgcgttg
      661 cggaaggtgg cgggatgaac ttttatcaag ataacccctt tgacacatga ttagaatgat
      721 gagacccggg ctgttgcgca atttgcagat cgggtttggg ctttccctgt tgatccttgt
      781 tatcacttcg gttgcttcct atagcagcat ccaaaacctg atggatggcg ccgggcgggt
      841 agatcatacc gattcggtgc ttatcaagat cgacaggatc gtggcggtat tgcgggacgc
      901 ggagtcgggg cagcggggct atctgcttac cggtgatgaa agctttctcg ccggatatga
      961 aggtgcgcag gaccggatga aggcgctggt cgacagcatc ggggagatga cggcggagaa
     1021 tccgcgggag gagaagaata tcgtggagct gcgccaagtg cttttcctgc ccctgaccac
     1081 gctccgggtg atgatcgacc agaagaagac ggacaatatc tataatacta ccgccctgat
     1141 gctggcccgg gagcagatgc aggatgcccg gaaggtgctg cagcggatgg aggacgagga
     1201 gcggaggctg atgagcagcc ggatcgacaa ggtgcggcgc tattccgcct atacgccggt
     1261 gctgatcctg acggcggcgc tgctctctat cctgatcacc attttctttt accggcgggt
     1321 gcaccaggat tatctcgagc gggctgccct gtatgcggag ttgcagcaaa aggatgccga
     1381 ggtggcgcgg cggatcgata ttatcagcga gatcgccgac cagataagcg acggccatta
     1441 ccaggtgagg gtctcggatg acatgaaggg cagtcttggg gagttgtcgg gttcgctcaa
     1501 caagatggcc gagtcactgg attattcgtt cgggttgttg tcggataaag agtggttgca
     1561 gagcggcatt gctaaacttt ccgatgagat ggtgggggag accgacctgc aggccctggt
     1621 gatgcatgtc atcgccttta tcgcagcata cagccatagc cgggtggggg cgttttatat
     1681 cctcgatccc gtggcgggag tgctgaccag ggccggagcc tttgcgctta gcggcgggga
     1741 cgaccgcgtg aaggtgaagc ttggggaagg gcttgtcggc caggtggcgc gggatgggaa
     1801 gatggttgtc ctgaacgata tccggcttgg ggaatggatc atccggctcg ccagcgggga
     1861 tattactccc cggaccgtgc ttgccttccc catctttcat gagaaaaagg tcaggggggt
     1921 gatcgagctg gggtcgctgg aaggttatgc tgagcgggac aaggaattct tcaccagtat
     1981 cggggacaat atcggtgccg ccatccaaag tatcgaaagc cggcagcggc tgcaggagct
     2041 gctggaggag acgcaggcgc aggccgagga gttgcagtcg cagcacactg agctcgagca
     2101 cctcaacctc gagctggagg tgcaggcgga aaaactacag gtctcggagg aagagctgaa
     2161 ggttcagcag gaggagctca tgcagaccaa ccaggagttg gaggagcgga gccgtacgct
     2221 ggaggagaag aaccagctga tcctgatccg caacctcgat atccagcgga aggccgagga
     2281 actggcgctg accacgaaat ataaatcgga gttcatggcc aatatgtcgc atgagctgcg
     2341 gacgccgttg aactcgatcc tgttgttgtc gcgactgttg tcggagaacg gcggcgggaa
     2401 tctctccgat gagcagatcg aatatgcccg ggtgatccag aactcgggtc aggggctttt
     2461 gcaactgatc gatgagatcc tcgacctttc gcggatcgaa tccggtaagc tcccgctcga
     2521 gcatgcgctt gtcaccgtga cggatgtcat cgatgatatg cggatgttgt tcgagcctat
     2581 tgcgaaggac aagaacctcg acctgcattt tgtcatcgag gaagggacgc cccgtcaact
     2641 ggaaacggac aagatgcggc tcgagcagat agtgaagaac ctgctggcga atgcgttcaa
     2701 atttacgcat aaggggtctg ttacgctgcg ggttcgtgtt tcggcggaac agaagaactt
     2761 cctcgagttc gtcgtccggg attcggggat cgggataccc gaggacaagc accggcttat
     2821 cttcgaggcg ttccagcagg cggatgggtc gacaaggcgg ttatatgggg gaacgggcct
     2881 tgggctgtct atcagccgtg agctggcccg gctgcttggc ggggacatcc gtgtcagcag
     2941 caggcccgat gagggcgccg agtttacggt gagtattcca cagttcaaga tggagcc
//
LOCUS       DATGFG010000470         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_917558, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000470 DATGFG010000000
VERSION     DATGFG010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917558"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..2100)
                     /locus_tag="VKQ52_08725"
     CDS             complement(466..2100)
                     /locus_tag="VKQ52_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HLZ87311.1"
                     /translation="MTLRAALVVLPVLFNCCVARAQGRLSGVRIGKTGNTAQLTIDKK
                     PFLILGGELGNSSASSMDYMRPYWEHLRQMHLNTVIAPVYWELLEPEENRFDYTLVDS
                     LINTARQYDLKLVLLWFGTWKNSMSCYTPVWMKTNARRFPRTADSAGRSQEIFSAFGK
                     ETLEADKKAFAALMRHIRETDAGQKTVLMVQVENEIGMLSTAREVSERANALYKGKIP
                     EELSGYLRKNKDRLVPELRKRWEENGARTDDSWENVFGKGVATEEIFQAWYYAKYANE
                     VALAGKKEYEIPMYVNAALPRPGVLPGRYPSAGPLPHLMDIWQAGAPAIDVLSPDFYN
                     PNTRYWCDLYVRNQNPLFIPEMQLDKAAAAKVFLAIGHYKAFGFSPFSIESGSDASAP
                     LSRSYDVLSQLSPLINSRKWIAMEGFVFDNKKKGDTLRMGDYSIALAHYNILPWAREG
                     RDSVWGMTGGLIIQTGADEFYVAGNGFVATFSNSDPARVTNIVSSDEVQYIDGREVKG
                     RRMNGDQDHQGRHIRFSTEDWGIQKVRLYNSPRDIE"
     gene            complement(2113..>3100)
                     /locus_tag="VKQ52_08730"
     CDS             complement(2113..>3100)
                     /locus_tag="VKQ52_08730"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87312.1"
                     /translation="EYNSEIMYSVERIVGDQNDDEVLSPTSFDPKTNIEGNLWTCNYQ
                     NSYDIPRGSKHFPVDRVIQYMRPLRQLIPTPYVYNVAFIDKVSDSRYDNTFRTVWQAT
                     NTNCSAAGINVGDTCFFLAPYKAYADSLKALGTKKYAILPPDSFYNAKSSAIQLYPSL
                     KKYDDNKRVGVSDYSGRPTPVARLGETYLLAAEAAIQAGQTSNALSLISTLRTRAAYR
                     AGLAPADLAARQAMMLMKNTGTAQSPVWSPLTTADMTLDFIMQERTRELFGENVRWAD
                     LACRGLLVSRVQAYNQLGGPNVKSTNVLRPIPQSLLDAITDPNKAQYQNPGY"
BASE COUNT          700 a          915 c          737 g          748 t
ORIGIN      
        1 cactgctcac gcccccggaa gagcccttga cggtaatagt ggccttgaca acaggctgac
       61 cgctctcgcc ggtgaccctg cccgttatat gaatgttgtt ttgcgccccg gcggccaggg
      121 caaagagaaa agtcgcgaga acacatactg caactctcat aagtgtttgt ttaaaatggt
      181 tggttaatat cggccggcag gcgccggcag gaatggcata tcaactgtaa gaagagaagg
      241 aaaagacaga tggcggcttg gcacgcatgg cccggaatcg cacacctttt aaaaaggaga
      301 gcctggtagt tggttttggc ggcatgaagc aatcgttttt tgaatggcag gcgcaactcg
      361 cgcccaatgg taatactaaa ttaagtaaaa atgtaaccga ttacaatttt ttcttacaaa
      421 ctctttgatt aaccccctgt taacaaggtg taaacgattg taatttcact cgatatcccg
      481 tggactattg tatagccgca ccttctgtat cccccaatcc tcggtggaga accggatatg
      541 ccggccctgg tggtcctggt cgccgttcat tcttcttccc ttcacctccc tgccatcgat
      601 gtattgtacc tcgtccgaac tgacgatatt ggtcaccctg gccgggtcac tattgctgaa
      661 agtggcgaca aagccgttgc ccgcgacata gaactcgtcc gcccccgttt ggatgatcaa
      721 tcccccggtc atcccccaca cggaatccct gccctccctc gcccagggaa gaatattata
      781 atgggccagg gcgatcgagt aatctcccat tcgcagggta tcgccctttt tcttattatc
      841 aaaaacaaac ccttccatcg cgatccattt ccgactattg atcaggggcg aaagctggct
      901 caggacatcg taactccggg ataagggcgc cgaagcatcg gacccggact ctatggagaa
      961 aggagagaac ccaaaagcct tgtaatgacc gatcgccaaa aacaccttgg ccgccgcggc
     1021 cttgtccagc tgcatctccg ggatgaacaa aggattttga ttccgcacat acaggtcaca
     1081 ccaataacgg gtattcgggt tgtaaaagtc gggggacagc acatcgatcg ccggtgctcc
     1141 cgcctgccag atatccatga ggtgtggcaa cggaccggcg ctcggatacc tgcccggcaa
     1201 cacccccgga cggggcaggg ccgcattgac atacatcggg atctcatatt ccttcttccc
     1261 cgccagggcg acttcattcg catatttcgc atagtaccag gcctgaaaga tctcctccgt
     1321 cgcaacgccc tttccaaaca cattctccca gctatcatcg gtcctggcgc cattctcttc
     1381 ccaccttttc cgtaattccg gcaccaggcg atccttattc ttccgcagat acccactcag
     1441 ctcttcgggg atcttcccct tgtaaagcgc attggccctc tctgagacct ccctcgccgt
     1501 cgaaagcatc ccgatctcat tttccacctg caccatcagg accgtctttt gcccggcatc
     1561 cgtctcccgg atatgtctca tcagcgcggc aaaggccttt ttgtcggcct cgagcgtctc
     1621 ctttccaaac gcagagaaga tctcctggct cctgcccgca ctatcggccg ttcgcggaaa
     1681 tcgtctggca ttggtcttca tccagacggg agtataacag ctcatgctgt tcttccatgt
     1741 cccgaaccat aacaacacca gcttcagatc atactgcctg gccgtgttga tcagactgtc
     1801 gacgagggtg tagtcgaacc ggttctcctc cggctccagc agctcccaat acaccggcgc
     1861 gatcaccgta ttgagatgca tctgccggag atgctcccaa tacggccgca tatagtccat
     1921 gctcgaggcg ctggagttgc ccaactctcc acccaggatc aaaaacggct ttttgtcgat
     1981 ggtaagctgc gcggtattcc cggtcttccc gatccggaca ccggacagcc tgccctgcgc
     2041 ccgcgcaacg caacaattaa acaataccgg caagacaaca agagctgctc tgagcgtcat
     2101 tctcaatact ttttaataac caggattttg atattgcgcc ttattcggat cggttatagc
     2161 atccagcagc gactggggaa tcggccgcaa cacattcgtg gatttgacat tcggcccacc
     2221 caactgatta tacgcctgaa cccggcttac caataaaccc ctgcaggcca gatccgccca
     2281 ccgtacattt tctccaaaca gttccctcgt tctttcctgc ataatgaaat caagggtcat
     2341 gtctgccgtt gtaaggggcg accagaccgg gctttgcgcc gtaccggtgt ttttcatcaa
     2401 catcatcgcc tgcctggccg ccagatccgc cggcgccagt cccgcccggt aagcggccct
     2461 cgtccttaac gtactgatca acgataaggc attggaggtt tgtcccgcct gtatggccgc
     2521 ttccgcagca agcagatagg tttcacccaa ccgggcaacc ggagtaggcc ggccggaata
     2581 atcactcaca cccactcttt tgttatcgtc atattttttc aggcttgggt acagttggat
     2641 cgcactgctt tttgcattgt agaacgaatc ggggggtaag atggcgtact tcttggttcc
     2701 caacgccttc agggaatcgg cataggcctt gtaaggtgcc aggaaaaaac aggtatcccc
     2761 gacattgata ccggcagcac tgcaattggt gttggtagcc tgccaaacgg ttctgaaggt
     2821 attatcatac cggctgtcac tgaccttgtc gataaacgcc acattatata cataaggcgt
     2881 ggggataagt tgtcgcaggg gccgcatata ttgtatcacc ctgtcgacgg gaaaatgctt
     2941 gctgcctctc ggaatatcgt aactgttctg gtagttgcag gtccacaggt tgccttctat
     3001 attcgtcttg ggatcgaagg atgtcggact cagcacctcg tcatcattct ggtcccctac
     3061 gatcctttcc accgagtaca tgatttcgct gttgtattca
//
LOCUS       DATGFG010000471         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_656505, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000471 DATGFG010000000
VERSION     DATGFG010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656505"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..2192)
                     /locus_tag="VKQ52_08735"
     CDS             complement(144..2192)
                     /locus_tag="VKQ52_08735"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HLZ87313.1"
                     /translation="MRKEKICLFFLSLFFLNFSLRAQELYVGADYHPHDNKDIAKIES
                     DIRLMQAAGFKVVRMGHLAWDSYEPTEGHFDFGWFDKVMALMDKAGIKVILDIAIRPA
                     PIWLHHKYPSIDVTDAGGNRLYPNHRYMEDVGDPMYQQYALRYADSLTRHYANYPALI
                     AFGIDNESGDGPISYSPSVRQRFIAWLRQKYGSTDSLDKAWAGQRWSRKIGDFDEIGL
                     PLSGNNIGAPERVLDFRRFVSDEVNGFLLKVIDKVNTNAPHALVNTNAWYYSQMKFFD
                     YTPIAYSGKMTREGCGFYPGNSLTTNYGVMDASFGIARIQFESTNPFWCTEFTTMTAV
                     PGSIRKSAYATLLYGNQMVCGWTWQSMHGGEEQYLEGMLDWDGTPNRKYEEYRKIATE
                     FKKIGKYFPYKLQAEVGLAFSFPSVIAGGYFPEQHQSQLQSCFDLFAYRNMDTRIIDI
                     GRSELHYKLVFLLGISVMDEATAARVRNYVKEGGTVVMTGNSAVVDATGKVFASTHPG
                     LLNDVFGIRVAGYEETESLNELSRKRLTGKKLELTYKGTAIPTEAARFEVIDPRGAEV
                     LGSITSLDKDYPIMTANRYGKGRAFYIGLPAKSELLGPLVDGLVDELSIRKGPAVPSG
                     VMARQIDKNHLLYLNITGEPKTIKGKGASRSILFDKDYQWDFVIPPYEPEFIETGARP
                     "
     gene            complement(2203..>2471)
                     /locus_tag="VKQ52_08740"
     CDS             complement(2203..>2471)
                     /locus_tag="VKQ52_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87314.1"
                     /translation="TVHIDHVPAGRYALEVYKVGYRCNDAYSAYLSMGRPSQLDRQEV
                     DQLKKQNDGSPVSREIITIQNGMPFLKELEMRENDVYFLNLIKL"
BASE COUNT          514 a          670 c          695 g          592 t
ORIGIN      
        1 tgaccaggtc tttcgcggga aagaaacggt cgatctgctg tgaccttgcc gttgttgccg
       61 acagcagcgc tgcaagcatg atcagtatgg cctttttttt catcaactat gctatttttc
      121 tatcgcggtc aaatggtttc aggtcaaggg cgagcgcctg tttcaataaa ctcgggctcg
      181 taaggcggga tcacaaaatc ccactgatag tccttatcga agaggatgct ccgggaggcg
      241 cctttgccct ttatcgtttt cggctcaccg gtgatattca aatatagcaa atggttctta
      301 tcgatctgcc gggccatgac gcccgatggc accgcggggc ccttccggat ggaaagttcg
      361 tcgaccagcc cgtcgaccag ggggcccaat agctcgcttt ttgcgggaag cccgatatag
      421 aaggccctgc ccttgccata gcggttggcc gtcatgatgg gataatcctt gtccaggctg
      481 gtgatgctgc ccagcacttc cgcaccgcgg gggtcgatga cctcgaaacg ggccgcttcg
      541 gtggggatag ccgtcccttt gtatgtaagt tcgagcttct tgcccgtcaa ccgctttcgc
      601 gacagctcgt tcaaggactc ggtttcctcg tagccggcca cccggatgcc gaatacgtcg
      661 ttcagcaacc cgggatgggt ggaagcaaag accttcccgg tggcgtccac taccgcggag
      721 ttgccggtca tcaccaccgt cccgccttct ttcacgtagt tgcggaccct ggcagcggtg
      781 gcttcatcca tcaccgaaat acccagcagg aagaccagct tatagtgcaa ttcgcttcgc
      841 ccgatgtcga tgatccgggt atccatattc cgataggcga acagatcgaa gcaggattgc
      901 agctggcttt gatgctgctc gggaaaatag ccgcccgcga tgacgctggg gaaggaaaat
      961 gcgagtccca cctcggcctg caatttgtag ggaaaatact tcccgatctt tttgaactcg
     1021 gtggcgatct tccggtattc ttcatatttc cggtttggcg tgccgtccca atccagcatt
     1081 ccttccaggt actgctcttc gccgccatgc atgctctgcc atgtccagcc gcagaccatt
     1141 tgattgccat atagtaaggt agcgtaggcc gacttccgga tggagccggg gacggccgtc
     1201 atcgttgtga actcggtgca ccagaaaggg ttggtgcttt cgaactggat ccgggcgatc
     1261 ccgaacgagg catccatgac gccatagttt gtcgtcaggg aattgccggg ataaaagccg
     1321 cagccctcgc gggtcatctt tccggaatag gcaatggggg tatagtcgaa gaatttcatc
     1381 tggctatagt accaggcatt tgtgttgacc agggcgtggg gagcgttggt attcaccttg
     1441 tcgatcactt tgagaagaaa cccgttgacc tcatcggaga cgaaccgccg gaaatccagc
     1501 actctttcag gcgcgccgat gttgttgccg gacaagggca gtccgatctc gtcgaagtcg
     1561 ccgatcttcc tggaccagcg ctggccggcc catgccttgt cgaggctgtc ggtgctgcca
     1621 tatttctgcc gcagccaggc gatgaagcgt tgccggacgg agggggaata ggagatcggg
     1681 ccgtctcccg actcattgtc gatgccgaag gcgatcaagg cgggataatt ggcataatgc
     1741 cgggtgaggg aatccgcata ccggagtgcg tattgctgat acatgggatc cccgacgtcc
     1801 tccatatacc ggtgattcgg gtagagccgg tttccgccgg catccgtgac atcgatggat
     1861 gggtatttgt gatgcagcca gatgggagcc ggccggattg caatatccag gatgaccttt
     1921 atcccggctt tatccataag agccataacc ttatcgaacc agccgaaatc gaaatgccct
     1981 tcggtgggtt catagctatc ccaggccaga tgccccatcc ggacgacctt gaatccagcc
     2041 gcctgcatga gccggatgtc cgattcgatc ttcgcgatgt ctttgttatc gtgggggtga
     2101 taatctgcgc ccacgtataa ttcctgtgcc cgcaaagaga aattcagaaa aaacagggac
     2161 aggaagaaca aacaaatttt ttcttttcgc atcagaattg tcttacagtt ttatcaggtt
     2221 gagaaaataa acatcgttct cccgcatctc cagctctttc agaaagggca ttccgttctg
     2281 gatggtgatg atctccctgc ttacgggcga gccgtcgttc tgttttttga gctggtcgac
     2341 ctcctgtctg tccagctgcg aaggccgtcc catcgacagg taggcggaat aggcgtcgtt
     2401 gcaacgatag ccaactttgt acacctcgag ggcatacctg ccggcgggga catggtcgat
     2461 gtgtaccgtg a
//
LOCUS       DATGFG010000472         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1385469, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000472 DATGFG010000000
VERSION     DATGFG010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385469"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VKQ52_08745"
     CDS             complement(<1..878)
                     /locus_tag="VKQ52_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ87315.1"
                     /translation="MKTSKVDIPRYDLDHFRHVHRVEDEPSVFGCNSLDRSRFIPGFE
                     LYSSMGLVSSAGPLRSEFYRISLTVTGTLDMQIGLERYRHQPRTMSFTFPNQIFSKNN
                     ISADAFGYYMLFKASFPEDVLPAGRLQTEFPFLSTTGTPLFQLSGEELAAVVELVKKM
                     DVEVRSDRPGRERAVQLYLYLLLLEARRSYERQGLDHPAPVPAGHGLVSRFQKLVGMH
                     FLSRRQVAEYAELLSVSANHLNKVVKEVTGKTASDSIREMLGQEAKSLLRYTDNSISE
                     IAYRLDFSDPASFNRF"
     gene            981..1388
                     /locus_tag="VKQ52_08750"
     CDS             981..1388
                     /locus_tag="VKQ52_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HLZ87316.1"
                     /translation="MTDIQSEADIKTLVDNFYAKVRVDDLLGPIFLSRLGEHWENHLA
                     TLYKFWGMALLGDNNYTGHPLSKHLPLPIEETHFNRWLHLFFETLTQHFDGPVAGEAM
                     KKAGLIARTFLARISAYQTGQPTIYPTPPSTDK"
     gene            1436..>3428
                     /locus_tag="VKQ52_08755"
     CDS             1436..>3428
                     /locus_tag="VKQ52_08755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HLZ87317.1"
                     /translation="MRKISHLSAASIRQILITTSILALFTLTLHAQPQKENIKVTDLL
                     RIKTVGEVRLNKEGTKAVFALTTIEPDNDPKASKWDYKYSTQLYIVPTDGSAAPRQLT
                     TKENATQPAWSPDGRQLAFVRNTDGKPQVFLLSFDGGEPAQLTHFRYGASSPKWSPDG
                     KEILFSARIGLKEMLKDSILNSAHALPAWPTEQAGIAKGTRPPTAKPNPDGSLEEIRA
                     YLDNDATDKKAKVVTKLNFQDEMEVNPETAFNQWFTLPAQPASPAQPGQPAPAPVPVA
                     FGFYNYTSVDYSPDGKKLVIVAHVDSSENPDRALESEIFIADRDGSHFHKLLGEKDKV
                     YGNPRISPDGRLIAYTLSPTSFVTVPQLYTMPLDEPGNTATKTLIPFDRAATGFTWSA
                     DNQYLYFIAQSNGGAPLYRANIATKKVEQLSDYNSGTTGFDLQAGRLVFGRTEVSDPS
                     ELYTADAAMHDAKLLTAFNGEWLQHRQLVLPEKHSFKNSLGQTVEYWVMKPANYTPGQ
                     HYPLLLDIHGGPSAMWGPGESSMWHEFQFFCAKGYGVVYCNPRGSGGYGLDFLRANIK
                     DWGAGPTSDVFTALDKAVAEGWADTSKLVVSGGSYAGYLVAWIVGHDHRFKAACAQRG
                     VYDLATFFGEGNAWRLVPNYFGGYPWEPATKAILQKESPI"
BASE COUNT          879 a         1211 c          730 g          608 t
ORIGIN      
        1 aaaaaccggt tgaacgacgc aggatcacta aaatcaagcc gataggctat ctccgagatg
       61 gaattatccg tataccgtaa aagcgacttc gcttcctgcc ccagcatctc ccgtatactg
      121 tccgacgccg tcttccccgt cacctccttt accaccttat taagatggtt ggcactcacg
      181 gacaacagct ccgcatattc agccacctgt cgcctcgaaa gaaaatgcat ccccaccagc
      241 ttctgaaacc gcgacaccaa cccatgcccc gccggcaccg gcgccggatg atccagcccc
      301 tgccgctcat aactgcgcct cgcctccagc agcaacaggt acagatacaa ctgcaccgcc
      361 cgctcccggc cgggacgatc actccgcacc tccacatcca tcttcttcac cagctccacc
      421 acggccgcca gctcctcccc ggaaagctgg aacaaaggcg tccccgtcgt actcagaaaa
      481 gggaactctg tctgcaaccg acccgcaggc aacacatcct cggggaaact cgccttgaaa
      541 agcatataat acccaaaggc atcagcactg atattgttct tcgaaaagat ctggttcgga
      601 aaagtaaaac tcatcgtccg cggctgatga cggtaacgct ccaggccgat ctgcatatcc
      661 aatgttcccg tcaccgtaag gctgatacga tagaattcag atcgtagcgg accggcactg
      721 gacaccaacc ccatactcga gtacaactcg aaccccggta tgaaccggct cctgtccagg
      781 ctgttacacc caaacaccga aggctcgtcc tccacccggt gaacatgccg gaaatggtcg
      841 agatcatacc tgggaatatc caccttgctg gtcttcataa tcccaaagat aatacgggca
      901 ccccatcaca caaataaaag ataaaattgt cctttattaa aatggtcttc ctatctttgc
      961 atcgtataaa agcgctcacc atgacagaca ttcaatccga agccgacatc aaaacactcg
     1021 tcgacaactt ctacgcaaag gtcagggtcg acgatctgct cggccccatc ttcctctcgc
     1081 gcctcggcga acactgggag aaccacctgg caaccctcta taaattctgg ggcatggccc
     1141 tcctgggcga caacaactac accggccacc ccctctcgaa acacctcccc ctccccatcg
     1201 aagaaaccca cttcaaccgc tggctgcatc tcttcttcga aaccctcacc cagcacttcg
     1261 acgggcccgt agccggcgaa gccatgaaaa aagccggcct catcgcccgc accttcctcg
     1321 cacgcatatc cgcatatcaa accggccagc ccaccatcta tccgaccccg ccatccaccg
     1381 acaaataggc gatttataca ggggattttg taagttgagg agacaaaact gacgcatgag
     1441 aaaaatatcc catttatcag ccgcctcaat tagacaaatc cttatcacca ccagcatcct
     1501 cgcccttttc acgctcaccc tccacgccca accccaaaaa gagaacatca aagtcaccga
     1561 cctcctccgc ataaaaacag tcggcgaggt ccgcctcaac aaggaaggca caaaagccgt
     1621 cttcgctctc accaccatcg aacccgacaa cgacccaaag gcctccaaat gggattataa
     1681 atatagcacg caactataca tcgtgccgac cgacggctcc gccgcccccc gccagctaac
     1741 gacaaaagaa aacgctaccc aacccgcctg gagccccgac ggccgccagc tcgccttcgt
     1801 tcggaataca gacggcaaac cacaggtatt cctcctttcc tttgacggcg gcgaaccggc
     1861 acagctcacc catttccgct atggcgcatc ctcgccaaaa tggtcccccg acggaaaaga
     1921 aatcctcttc tcggcccgca tcggcttaaa ggaaatgctg aaggactcca tcctcaacag
     1981 cgcacatgcc cttcccgcat ggcccacgga acaggccggc atcgcaaaag gcacacgccc
     2041 acccaccgca aaaccgaacc ccgacggcag cctggaagag atcagggcct acctcgacaa
     2101 cgatgccacg gataaaaaag caaaagtggt gacaaaactc aatttccagg atgagatgga
     2161 agtaaacccg gaaacagcct tcaaccaatg gttcaccctg cctgctcaac cggcttcccc
     2221 ggcccagcca ggccagcctg cccctgcccc cgtacccgtt gccttcggtt tctacaatta
     2281 cactagcgtc gactactccc ccgacggtaa gaaactcgtc atcgtcgccc atgtcgattc
     2341 ctccgaaaac cccgaccgcg cactggagag cgagatcttc atcgccgacc gtgacggcag
     2401 ccatttccat aaactgctgg gagaaaaaga caaggtctac ggcaacccac gcatcagccc
     2461 cgacggccgc ctcatcgctt acaccctcag ccccacctcg ttcgtcaccg tgccgcagct
     2521 ctatacaatg ccactcgacg aacccggcaa cacagccacg aaaaccctga tccctttcga
     2581 tcgcgccgcc acaggcttca cctggagcgc cgataaccaa tacctctatt tcatcgctca
     2641 atccaacggc ggcgccccac tctatcgcgc aaacatcgcc acaaaaaagg tggagcagct
     2701 ctccgactat aacagcggaa caaccggctt cgacctgcag gccggtcgcc tggtcttcgg
     2761 caggaccgaa gtatccgacc cctctgagct ctacaccgcc gacgccgcca tgcacgatgc
     2821 aaaactcctc accgccttca acggcgaatg gctccaacat cggcaactcg tcctgcccga
     2881 aaaacattcc tttaagaata gcctcggcca gaccgtcgaa tactgggtca tgaaacccgc
     2941 caactatacc ccgggacaac actaccccct gctcctcgac atccatggcg gtccgtcagc
     3001 catgtgggga ccgggtgagt cttccatgtg gcatgaattc cagttcttct gcgcaaaagg
     3061 ctatggcgtc gtctactgca acccccgcgg ctcgggtggc tatggcctgg atttcctgcg
     3121 ggccaatatc aaggactggg gcgccggacc gacaagcgat gtcttcaccg cactcgataa
     3181 agccgtggcc gaaggctggg ccgatacaag caaactggtc gtcagcggag gctcctacgc
     3241 cggctatctc gtagcctgga tcgtcggtca cgatcatcgc ttcaaagccg catgcgcgca
     3301 acgcggcgtc tatgatctgg ccaccttctt cggcgaaggc aacgcctggc gcctggtgcc
     3361 gaactacttc ggcggctatc catgggaacc cgcgacaaaa gccatcctcc agaaagaatc
     3421 ccccatca
//
LOCUS       DATGFG010000473         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_209406, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000473 DATGFG010000000
VERSION     DATGFG010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209406"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VKQ52_08760"
     CDS             complement(<1..1192)
                     /locus_tag="VKQ52_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HLZ87318.1"
                     /translation="MTTRLLIAGLSLVLLQTAAKAQDKMPVKFGKVTPQDFAVTAPGP
                     DSAAGAIVIADFGTSSFEGNTQGWFSLIYKHSCRIKILKREAFDAATITIPLYISGRE
                     VEKVESLRASTYNLEDGKVVETKLDDKSVFTNQLDKNHSEKKFSFPALKEGSIIEYTY
                     TQVSPFLFNLQPWAFQREYPCLWSEYQAEMPNFFQYVTLGHGFVPFSINTNDTRNVAF
                     HVTIPGGADRNERYDFDDNVVTHRWVMKNVPALKEEPFTTTVGNYMARLEFQLARYAF
                     PNGVNKDVMGNWTGLSKELLESDEFGADLTKGNGWMDDELAGITKGAGSQLEKTRKIF
                     AFVRDNFNCTSHSRLYMDNNLRTVFKNRNGSEAEINLLLTAMLIHAKIGAEPVILSTR
                     ANGFP"
     gene            complement(1221..3266)
                     /locus_tag="VKQ52_08765"
     CDS             complement(1221..3266)
                     /locus_tag="VKQ52_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3858 domain-containing protein"
                     /protein_id="HLZ87319.1"
                     /translation="MIALKPLNRILTILCLCLLHDLCSAQDKAKNTWGKISSADFSLP
                     GGSTIIDSTTSAVILADEGSVHYIGSKSGWFSYVYTRQTRIRILSKKVLGPGHDLSTV
                     SLRLYGRDENLEKLSKVEASAYNLENGQAVQTKLQDKDVFMTKLDKEHTEAKFTIPGA
                     KEGAILEYTYTITSDYWHLLPSWQFQWQEYPCLHSQYEVEIPQTLSFIFVRQGVHPYA
                     TDKGSEGRGSYKIREKIESLATQNEDRYFTINTIKHTWVMKDIPAFGAERYLSTPENY
                     VDKIDFQLSRTNNGETFEDQTNTWAKATEELLKREDFGGALAQDNDQLTELAGKAAGS
                     GADGLAQAKAIYYYVSHHFTCTNYYDKYIKTNLGDVLKRNSGTVGDINLLLIAMLRAK
                     GLRADPVVLSTREFGFNLASYPVLGRLNYVIARLKIEGKVYYLDAAEPQLGFGQLAGN
                     CYNGHARIISNTDSGSVWFLSDSLREKKLTMVLITGTDKGLEGQWQSTLGQEESYEVR
                     RDIDKRGETAYFKDIQTSWGDDMDIHDGGIDSLDKPEDPVKVHYEFLLKQTPGASVFY
                     LTPMLGEGYRENPFQAAERKYPVEMPYAIDETYIFSMEVPADYTVDELPKSAKVAFNG
                     DQGYFEYLIQQGGGQVQMRCRIRLNRAVFLPEDYSALRDFFAFVVKKESEPIVLKKK"
BASE COUNT          696 a          898 c          959 g          861 t
ORIGIN      
        1 tggggaagcc attcgcccgg gtactgagaa tgacgggctc ggcgccgatc ttcgcatgga
       61 tcagcatggc cgtgagcagc aggttgatct cggcctcgct gccgttcctg ttcttaaaca
      121 ccgtccggag gttattgtcc atgtatagcc ggctatggga agtgcagttg aaattgtccc
      181 ggacgaaggc aaagatcttc cgcgtctttt ccagctggga gccggcgcct tttgtgatac
      241 ccgccagttc gtcgtccatc cagccgttgc ctttggtaag atcggcgcca aactcatccg
      301 actccagcaa ttccttgctg aggccggtcc agttacccat gacgtccttg tttacgccgt
      361 tggggaaggc atagcgggcg agctggaatt ccaatcgggc catataatta cccaccgtgg
      421 tggtaaaggg ctcctctttc agcgcgggta cattcttcat cacccaccgg tgggtgacca
      481 cgttatcgtc aaagtcgtat cgctcgttcc tgtctgcgcc accggggatc gtcacgtgga
      541 aggcgacgtt ccgggtatcg ttggtgttga tgctgaacgg cacgaagcca tgaccaaggg
      601 tcacatattg aaagaagttc ggcatctccg cctggtattc gctccacaga caggggtatt
      661 cccgctggaa ggcccagggt tgcaggttga aaagaaaggg tgacacctgg gtataggtat
      721 attcgatgat ggagccttcc ttcagggcgg ggaacgagaa tttcttttcg gaatggttct
      781 tgtcgagctg gttggtaaac acggatttgt cgtcgagctt tgtctctacg acctttccgt
      841 cctccaggtt gtaggtgctg gcccgtaaac tctccacctt ctccacttcg cggccgctta
      901 tgtagagcgg gatggtaatc gtggccgcgt cgaaagcttc ccgcttcagg atctttatcc
      961 ggcaactgtg tttgtaaatg agcgagaacc agccctgggt attcccttcg aaggatgatg
     1021 ttccgaaatc ggcgatgacg atggccccgg cggcggaatc cggtcccggc gcggtaacgg
     1081 caaaatcctg gggagtgact tttccaaatt ttacgggcat tttgtcctgc gcctttgcgg
     1141 ctgtctgcag cagcacaagc gaaagcccgg caatgagcaa tcgggtggtc atgggagtag
     1201 ggggttatag gtgataggaa ttatttcttt tttagtacga tgggttcgct ttcctttttg
     1261 acgacgaagg cgaagaagtc gcgaagcgcg ctgtagtctt ccggcagaaa gacggccctg
     1321 ttcagccgga tcctgcaacg catctggacc tggccgcctc cctgctgtat caggtattca
     1381 aaatatccct ggtcgccatt gaatgccacc ttggccgact tgggcagttc gtcgacggtg
     1441 taatccgccg ggacctccat cgaaaaaata taggtttcat cgatagcata gggcatttcc
     1501 acgggatact ttcgctccgc cgcctggaag ggattctccc ggtatccctc gccgagcatc
     1561 ggggtcagat agaataccga ggcgcccggg gtctgcttca gcaggaattc atagtggacc
     1621 tttaccgggt cttcgggttt gtcgagggaa tcgatgccgc cgtcgtggat atccatgtcg
     1681 tcgccccaac tggtttggat atccttgaaa taggccgttt cccctctttt gtcaatgtcc
     1741 ctcctcactt cgtaagattc ttcctggcca agcgtggact gccattgtcc ctcgaggccc
     1801 ttatcggtgc cggtgataag caccatcgtc aatttctttt ccctgagcga gtccgaaagg
     1861 aaccacacgg accccgagtc ggtattgctg atgatccggg catgtccgtt ataacagttg
     1921 cccgcgagct gtccaaaccc cagctgaggt tccgccgcgt caaggtaata gaccttgccc
     1981 tctatcttta gccgcgcgat cacataattc agccgcccca acacgggata ggaggccagg
     2041 ttgaacccga attcccgggt gctcaatacg acagggtcgg cccgcagccc ttttgcccgg
     2101 agcatggcga tgagcaggag gttgatgtca cccaccgtcc cgctgtttct cttcaggaca
     2161 tcgcccaggt tggtctttat atacttatcg taataattgg tacaggtgaa atgatggctt
     2221 acatagtagt agatggcttt tgcctgggcc agtccatcgg cgccggagcc cgcggccttc
     2281 ccggccagct ccgtcaactg gtcattgtcc tgcgccaggg ccccgccaaa gtcttccctt
     2341 ttcaggagct cttccgtcgc cttggcccag gtgttggtct ggtcttcgaa ggtttcgcca
     2401 ttgttggtcc gcgatagctg gaagtcgatc ttgtcgacat agttttccgg cgtggagaga
     2461 tagcgctcgg cgccgaaagc cgggatgtcc ttcatgaccc aggtatgctt gatggtgttg
     2521 atcgtgaaat accggtcctc attctgggtt gccagactct ctatcttttc ccttatctta
     2581 taggaaccgc ggccttccga tcccttatcc gtggcatagg gatggacacc ctgccggaca
     2641 aagataaagg acagggtctg ggggatctcc acctcgtact ggctatggag acaggggtac
     2701 tcctgccatt ggaattgcca ggagggaagc agatgccagt agtccgatgt tatcgtatag
     2761 gtatattcga ggatcgcccc ttcctttgcc ccggggatgg tgaattttgc ctcggtatgt
     2821 tccttgtcga gtttggtcat aaagacgtcc ttgtcctgca gcttggtctg taccgcctgt
     2881 ccgttctcca ggttgtaggc ggaagcctct accttgctga gcttttcgag gttctcgtcc
     2941 cggccataga gccggaggct gacggtggag agatcatggc cggggccaag gaccttcttg
     3001 ctgaggatcc tgatccgggt ctgtcttgta tagacatagg agaaccagcc gctcttgctg
     3061 ccgatataat gaacggaacc ctcatcggcg aggatgacgg cgctggtggt gctatcgatg
     3121 atggtggagc cgcccggaag ggagaagtct gcggagctga tctttcccca tgtattctta
     3181 gctttgtcct gggcggaaca aaggtcgtgt aagagacata ggcataaaat cgttagtata
     3241 cggttcaatg gcttcagggc gatcatatga ggtgaggtta gggaataaac ggtcctaaaa
     3301 taaataaatt gcttatatat tcccaccccc tggggataaa aaaataacac ttgacacaaa
     3361 acaaccatac atacctgatt aaaaaatgga gccaggaatt gggttttcaa cact
//
LOCUS       DATGFG010000474         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1170280, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000474 DATGFG010000000
VERSION     DATGFG010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170280"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /locus_tag="VKQ52_08770"
     CDS             <1..1771
                     /locus_tag="VKQ52_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87320.1"
                     /translation="NANAVQAYMADIYTNLPMEDFVYEPNAGFLTGNGGRWQNFYHTD
                     AIDGEMAGPYGGTGDAATGFGFWPYDKIRVINVLISTLPKYAVNFTQDQVNEWLGEAY
                     FCRAYTYFALAKRYGGVPIVRTVVGPNASPASLQVPRDKEEDVYTFIASDFDSAFALM
                     SDPGSKIPQGAASKYAAPAYKSRAMLYAGTIAKYGAINYVDGDARAKGFAGIPATDAG
                     KFFQAAYDAAKLVEGHFSLYTGGYPDKQQNYVDLFLKPNNEDIFLRQYTQSDPNQNGD
                     NAGPHSWDATMTCRIMTADGLSRAYPSEDFVERFGALPVVNPDGTPVRFTNRADLMNG
                     LEPRLLATVYFPGASLRGISFDVQRGIYKTFTGSAAQELNNSLTSPNDVQTGPNVNLW
                     LGDINAVANGLDVIGRAGASTNGDDLTRTGFYVRKYVDYNKPASACGLYQSTQSYIDM
                     RYAEVLLNRAEAAMELGTGAMQADALSDINQVRDRAGAVPATVSMTIDTIRNERCKEL
                     AFEHQYWWDIRRWRTADQVLNNRNLYALLPYFITSENKYIFLKQLEPFKRTYNFQKLW
                     YYEPLPGGELGKNPNLFPNNPGY"
     gene            1810..2532
                     /locus_tag="VKQ52_08775"
     CDS             1810..2532
                     /locus_tag="VKQ52_08775"
                     /inference="COORDINATES: protein motif:HMM:NF037704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3823 domain-containing protein"
                     /protein_id="HLZ87321.1"
                     /translation="MNKLLIKGIIGAGFLLLGFSCTKTDNVAGPDSAFQGNIIDSVTG
                     KNLVTETGGVQIELKQLSWSANPDPYYIPSKPDGAFEDTRIFSGHYSVIPTQGAFWPV
                     DSILKDIKGPTSQNFTVVPYLEIINVTHTQNADSLVMTFQLQAPRTNGLPQIIDATEF
                     VNNTPFVGGGAFIQSFFNESSATVSINSNWSSAIGTTTYRTVVHNLQPGWTYYARIGV
                     RLNDSYKKYDLSDIVQVALPVK"
     gene            2549..>2878
                     /locus_tag="VKQ52_08780"
     CDS             2549..>2878
                     /locus_tag="VKQ52_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87322.1"
                     /translation="MNRRIVQRMVSVVATFVSTVPFLCSAQNPFVTDIYTADPSAHVW
                     ADGRLYVYPSHDVAPPRGCDLMDKYHVYSTDDMVHWKDHGQILESSQVPWGRPEGGFM
                     WAPDCAFK"
BASE COUNT          692 a          769 c          786 g          631 t
ORIGIN      
        1 caatgctaac gccgtgcaag cctacatggc cgatatttat accaatctgc ccatggagga
       61 ttttgtgtat gagccgaacg ccggcttttt gacgggcaat ggaggacgct ggcagaattt
      121 ttatcacacc gacgccatcg acggggaaat ggcaggccct tacggcggca ccggcgatgc
      181 ggcgacgggt tttgggttct ggccttacga caagatccgc gtcatcaatg tcctgatcag
      241 cacgctgcca aaatatgccg tcaacttcac acaggaccag gtgaacgaat ggctgggcga
      301 agcctatttc tgccgggctt acacttattt tgcattggca aaacgttatg ggggtgttcc
      361 gatcgtcagg accgtggtcg gtcctaacgc gagtcctgcc tccctgcagg tgccccgcga
      421 caaggaggag gacgtatata ctttcatcgc ttcggacttt gacagcgcat tcgccctgat
      481 gagcgatccc ggtagcaaga tcccgcaggg tgctgccagc aaatatgcgg cgccggctta
      541 taaatcgagg gccatgttat acgcagggac tatcgccaaa tacggagcca tcaattatgt
      601 agacggagac gcaagagcaa agggttttgc gggtatcccc gctaccgacg ccggcaagtt
      661 tttccaggct gcctatgatg cggccaagct ggtggaggga catttcagcc tgtatacggg
      721 aggatatccc gacaagcagc agaactatgt ggatctcttt ttgaaaccca ataacgagga
      781 tatcttcctc cggcaatata cccagtccga ccccaaccag aatggcgaca atgccggccc
      841 gcacagctgg gatgctacca tgacctgtcg cattatgacg gcggatggtc tttcaagggc
      901 ctatccttcg gaagattttg tcgaacgctt cggcgccctg ccggttgtca acccggacgg
      961 tacgcctgta cggtttacga acagggcaga cctcatgaac gggctggaac cccgcctcct
     1021 ggcgaccgtt tattttcctg gtgcgagctt gagggggatc agcttcgacg ttcaaagagg
     1081 gatctataag acctttaccg gcagcgccgc acaggagctg aacaattccc tgacctcgcc
     1141 caatgatgtc cagaccggac cgaatgtcaa cctgtggttg ggcgatatca atgccgtagc
     1201 gaacgggctg gatgtaatcg gacgggcagg cgccagcacc aatggcgacg atctcacccg
     1261 cacgggtttt tatgtgcgga aatacgttga ttataacaag ccggcgtccg cctgcggtct
     1321 ttaccagagc acacagagct atatcgacat gcgctatgcg gaagtgctgc tgaaccgggc
     1381 agaggccgcc atggaactgg gcaccggcgc tatgcaggcc gatgcgctta gcgatatcaa
     1441 ccaggtcagg gaccgtgcag gggccgtacc cgctacggtc agcatgacca tcgatacgat
     1501 ccgcaacgaa cgctgcaagg agctggcatt cgagcaccag tactggtggg atatccgcag
     1561 atggaggacg gccgaccagg tgcttaataa tcgaaatcta tatgcgctgt tgccttattt
     1621 tatcacatcg gagaacaaat acatcttctt aaagcagctg gagccgttca aacggacgta
     1681 taatttccag aaactctggt attatgagcc cctaccgggt ggagagctgg ggaagaaccc
     1741 caacctgttc cctaataacc cggggtatta acgagcgaag aaaggtaaac atccttaaaa
     1801 gaaattttta tgaacaaact attgataaaa ggaatcattg gcgccgggtt tctgcttctg
     1861 ggattctcct gcaccaaaac agacaatgtt gccgggccgg actcggcatt ccagggaaac
     1921 atcatcgaca gcgtcaccgg gaaaaacctg gtaacggaaa cgggaggcgt ccagatcgaa
     1981 ctgaagcagc tgagttggag cgccaacccg gatccctatt atatcccttc caaaccggac
     2041 ggcgcatttg aggatacgag gatcttcagc ggacattaca gcgtgatccc tacgcagggc
     2101 gctttctggc cggtggacag tatcctgaag gatatcaagg gccccaccag ccagaacttt
     2161 acggtagttc cctacctgga gatcatcaac gttacgcata cgcagaatgc ggactcgctg
     2221 gtaatgacct tccaactgca ggcgcccagg acaaacgggt taccgcagat catcgatgca
     2281 accgagttcg tgaacaatac gccctttgta ggcggcggcg cttttatcca gtctttcttt
     2341 aacgagtctt ccgccacagt gagcatcaac agcaactgga gcagtgccat cgggacgacg
     2401 acgtatcgga cagtcgttca taacctgcag cccggctgga cttattatgc aaggatcggc
     2461 gtccggctga acgacagcta taagaaatat gatctgtcgg acatcgtcca ggtggctttg
     2521 cctgtgaaat agtcaacaat tgttttttat gaatagacga atcgtccaaa gaatggtatc
     2581 cgttgtcgca acattcgttt cgaccgtccc tttcctgtgc agcgcacaga acccgtttgt
     2641 taccgatatc tatacggcgg acccgtcggc tcatgtctgg gctgatggcc gcctctatgt
     2701 gtatccttct catgatgttg ctccgccgcg tggatgcgac ctgatggaca aataccatgt
     2761 gtattccacc gatgatatgg tgcactggaa agaccatggt cagatcctcg aatcaagcca
     2821 ggttccctgg ggccggccgg aaggtggctt tatgtgggct cccgattgcg cgtttaag
//
LOCUS       DATGFG010000475         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_510492, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000475 DATGFG010000000
VERSION     DATGFG010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510492"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VKQ52_08785"
     CDS             <1..719
                     /locus_tag="VKQ52_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87323.1"
                     /translation="PPRKRNRILHLLGEMWPAYLIEVVVIILGISITLVLEEWRDSGK
                     EEKLERVYMQNLLGDVNADLESLKYALTSTEAILGRGDELLRFAKDPVRFPLTSHQVN
                     ADVRAILGRPKFIGHDATFSDLKSSGNLHLVNDISLKNLLFGYYSQAQGIKENQDAEQ
                     QATITLSGAYFLRYFALDDPGGAPAQTDTIGVRALAKNLEFRNNVLLRVTNRKELQAL
                     YQAANALALQLQAALLKKTS"
     gene            complement(723..1550)
                     /locus_tag="VKQ52_08790"
     CDS             complement(723..1550)
                     /locus_tag="VKQ52_08790"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLZ87324.1"
                     /translation="MSFPPLFRISLWSAWLLLLAACSANPYKVTNRSYKKQARQYAQT
                     ISEHPLPTGADSVPQSPYWVGTTNFNLRKPNFVIIHHTAQNSCPQTLQTFTLPRTQVS
                     AHYVICRDGTVYHLLNDYLRAWQAGVSKWGNITDVNSISIGIELDNNGFEPFPPAQIN
                     SLLHLLAKLKTAYGIPAANFIGHGDIAPVRKNDPSSFFPWKDLADKGFGLWYADTTGI
                     CVPAGFSSLTALRIIGYDIRDSSAAAAAFKRHFEQDTTRCWGEPEQKVLYSLYRQYQ"
     gene            1634..2329
                     /locus_tag="VKQ52_08795"
     CDS             1634..2329
                     /locus_tag="VKQ52_08795"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HLZ87325.1"
                     /translation="MITFCSLVTGGARLAAPRADESILGVVVDPQKQDLRFYWKDDSG
                     QLFRSIGHLKDWLGQRHKKLVFAMNGGMFRADNSPVGLFVQRGITVTPMDTAAGEGNF
                     YLQPNGVFYLTRDRKAGICPAKAFRDTGQITYATQSGPMLLIGGAFHPAFRKGSANVN
                     IRNGVGILPDHTLLFALSTEPVNFYDFAAYFKSRGCLDALYLDGFVSRAYLPEKDWTQ
                     TDGNFGVIMGVEE"
     gene            2410..2802
                     /locus_tag="VKQ52_08800"
     CDS             2410..2802
                     /locus_tag="VKQ52_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87326.1"
                     /translation="MKHLSILLMLSLLTAVLPTAKAQSLINTHWKAYIGDPVNDSLVL
                     HIKTDSSYVTDRNGTAVVRSVCKISADTLSLTDYDGEYACPSMTGRYKVAQSGGMLIF
                     TLIDDPCDGRAAALPLTKWRKVPDAGSK"
     gene            3087..>3329
                     /locus_tag="VKQ52_08805"
     CDS             3087..>3329
                     /locus_tag="VKQ52_08805"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ87327.1"
                     /translation="MKARRDVFQAIADPTRRAIIGLLAGQTLTLNTVAENFNMSQPAV
                     SKHMRILTECGLVHIVKRGRERHCSAQLTALEEVVRW"
BASE COUNT          745 a          807 c         1003 g          774 t
ORIGIN      
        1 atcctccccg gaaaaggaac cgcatcctcc acctgttggg agagatgtgg ccggcttatc
       61 ttatcgaggt ggtggtcatc atccttggta tttctatcac tctcgtgctg gaagagtggc
      121 gggacagtgg taaggaagaa aagctggaaa gggtatatat gcagaatctg ctgggggatg
      181 tgaatgccga tttggaatcg ttgaaatatg ccctgaccag tactgaggcc atccttgggc
      241 ggggagacga actgcttcgg ttcgcaaagg atccggtacg gtttccgctc acttcccatc
      301 aggtgaacgc cgatgtgcgg gcgatcctcg gacggccgaa attcatcggc cacgatgcca
      361 ctttctccga tctgaagagt agcgggaatc tgcacctcgt gaatgatatt tcactaaaga
      421 acctcctgtt tggctactat agccaggcac agggcataaa agagaaccag gatgcggaac
      481 aacaggccac catcacgctg agcggcgctt attttctccg gtattttgcg cttgacgatc
      541 cgggcggcgc acccgcgcag acggatacca ttggtgtcag ggcccttgca aagaaccttg
      601 aattccggaa taacgttctt ttgcgggtaa ccaaccggaa ggagttacag gcgctttacc
      661 aggcggccaa cgcacttgcc ctgcagttgc aggcggcgtt gctcaaaaag acctcctgat
      721 ccttactggt attggcggta cagggagtag aggacctttt gttccggctc tccccagcaa
      781 cgggtggtat cctgttcaaa gtgccgttta aaggccgcgg ccgcggcgct gctatccctt
      841 atatcatagc cgatgatccg gagggcggtg aggctgctga agccggccgg gacgcatatc
      901 ccggtggtat ccgcgtacca aagcccgaag cccttgtccg cgaggtcctt ccaggggaag
      961 aagctgctgg ggtcgttctt acgcaccggc gcgatatcgc cgtgaccgat aaaattggct
     1021 gcggggatac cgtaggccgt cttcaatttt gccagcagat gaagcaggct attgatctgc
     1081 gcggggggga agggttcgaa gccgttgttg tccagttcga tcccgatgga aatggagttg
     1141 acgtcggtga tatttcccca tttgctcacg ccggcctgcc aggcccttaa ataatcattc
     1201 agcaggtgat agacggttcc gtcgcggcag atgacatagt gtgcgcttac ctgggtacgg
     1261 ggcagggtaa aggtctgcag ggtctgtgga cagctgttct gggctgtatg gtggatgatc
     1321 acgaaattcg gtttgcgcaa attgaagttg gtggtgccca cccaatacgg tgactgcggc
     1381 acggaatcag cgccggttgg aagggggtgc tcactgatcg tttgggcata ttgccgggcc
     1441 tgttttttgt aggagcggtt ggtgaccttg tagggattgg ccgagcaggc ggccagaagg
     1501 agaagccagg cggaccacag ggatatgcgg aagagaggag ggaaggacat agaaaaaaat
     1561 tagctaaggg tgtaagataa aaaaaaataa agtagattga acccgaaaca tgcgggtcgt
     1621 catattatcg atattgataa cgttttgctc cctggtgacg ggtggcgccc gtcttgctgc
     1681 cccgcgggcc gatgaatcca tccttggcgt tgtcgtcgat ccgcaaaagc aggacctccg
     1741 gttctactgg aaggatgaca gcgggcagct cttccggagt atcgggcatt tgaaggactg
     1801 gctcggacag cggcataaga aacttgtctt tgcaatgaat ggcgggatgt tcagggcgga
     1861 caattcgccg gtggggcttt ttgtccagcg gggtattact gttacgccga tggacaccgc
     1921 cgcgggagaa gggaattttt atctgcagcc aaacggggtg ttctatctga cgagggaccg
     1981 gaaagcaggg atctgtcctg caaaggcttt cagggatacg gggcagatca cctatgcgac
     2041 gcagtcgggg ccgatgctgc tgatcggcgg ggcttttcat cctgcgttcc gaaagggttc
     2101 cgccaatgtc aatatccgga atggcgtcgg catcctgcct gaccatacgc tgctttttgc
     2161 cctttcaacc gagccggtga atttctacga ctttgcggcc tacttcaaaa gcaggggctg
     2221 cctggatgcc ttatacctgg atggttttgt ttccagggct tatctgccgg agaaggactg
     2281 gacccagacg gatgggaatt ttggcgtgat catgggtgtt gaggaatgag ctgaccggcg
     2341 tccgtcgcgt gttgtgaatg gtttggccga aggttttttt ctcagtactt tacttataaa
     2401 acctccgtga tgaaacacct ttctatcctg cttatgctct ctcttctgac ggccgtcctc
     2461 ccgacggcaa aggcacaatc gctgatcaac acgcactgga aggcatatat cggcgacccg
     2521 gtgaacgatt cgctggtcct gcatatcaag accgattcct cgtatgtgac cgaccgtaat
     2581 ggtactgcgg tggtgcgttc tgtttgtaag atctccgcgg acacgctctc gcttaccgat
     2641 tatgacgggg aatatgcctg tcccagtatg acggggaggt ataaggtggc gcaatccggc
     2701 ggcatgctca tttttaccct gatagatgat ccctgtgacg gaagggcggc ggcgctgccc
     2761 cttacaaaat ggaggaaagt gccggacgcc ggaagcaaat gacaccagga ggccggagcc
     2821 ccggtcatac cctgaatatc ttgttcagca tgcgggccgt ctggcggatg gagtcggtca
     2881 gttgtcgtgg gtctgctttt ggtgattccg gattgttctt tgaaatatcc gggccaaata
     2941 tacataagat atagatgcat ccaaaataaa tatatacacc agcccatttc ctgaaaaatg
     3001 gcggcgggaa aaaaataatt tggtaaaccg aatggtttat tatagtttta ctaaaccatt
     3061 cggtttagta ttataaacgc tatcgcatga aagcaagaag agatgtcttc caggccattg
     3121 ccgacccgac gaggcgcgcc atcatcgggt tgctggccgg gcagacgctg acgctgaata
     3181 ccgtcgcgga gaacttcaac atgagccagc cggccgtctc caagcatatg cgcatcctga
     3241 cggaatgcgg gctggtgcat atcgtcaaac gggggaggga acggcattgc agcgcccaat
     3301 tgaccgcgct cgaagaggtg gtgcgatgg
//
LOCUS       DATGFG010000476         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1462818, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000476 DATGFG010000000
VERSION     DATGFG010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462818"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VKQ52_08810"
     CDS             complement(<1..1267)
                     /locus_tag="VKQ52_08810"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HLZ87328.1"
                     /translation="MKKIININFHSRVIPIEETAYEMLQQYIESLRRYFANEEGKEEI
                     ISDIENRFAELFSETLKKGAACITDADVNTIIASMGRPEEFEGESTAEAAPAGSTAGQ
                     QQSGASQPNWQFAGDEHRRLYRAGNDKILGGVCAGLANYLRLDPAIVRIIFVLMTLGW
                     GSGVLLYIILWIVLPPRSLPVNVRKRLYRNSDDRIIGGVASGLAAYFHIDVWIPRLIF
                     ALPLILSVLTSIMRNVWWFHRWGGPEFFTGGFGGTLFITYIVLWIVLPEAVTASEKLE
                     MRGEKVDLESIKNTIKSDLESFKGRAKEMGTEMRESFQQVGSQVKQSTQAWAAEAGPA
                     IRKSGTGFGHAVGVLFKAFFLFIAGIIAFSLIMVLIGLAFRGDGLLHLKGYVLEGFWQ
                     NFLAWSGFFLFLVIPVIALLTWLIRRISGT"
     gene            complement(1310..1642)
                     /locus_tag="VKQ52_08815"
     CDS             complement(1310..1642)
                     /locus_tag="VKQ52_08815"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLZ87329.1"
                     /translation="MNIENTQSQMRKGILEFCILSVIRRGEAYPSDIVEEMKSANLNI
                     LEGTLYPLLTRLKNADMLTYRWVESNSGPPRKYFSLTPKGEAFYGELEATWTELANAV
                     HALTNNKH"
     gene            complement(1934..2878)
                     /locus_tag="VKQ52_08820"
     CDS             complement(1934..2878)
                     /locus_tag="VKQ52_08820"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HLZ87330.1"
                     /translation="MAIPVVDLSEFTSGTPQQKAAFVSALGKAYEEVGFVAVKNHGIP
                     DATVAGLYSNTQAFFSQPQDIKRKYEIAGLAGQRGYTSFGKEHAKGSTAPDLKEFFQF
                     GQTVEDNDPIKAEYPDNVQVAELPDFNKLFSKAYRSFETSGTQLLRAIALYLGLDENY
                     FDPYIHNGNSILRAIHYPPIKEEPRSAIRAEQHEDINLITLLVGASADGLEILSKKNE
                     WVPVTSLPDQIVVNVGDMLQRLTNNKLKSTTHRVVNPPREHWGSPRFSIPFFLHPKSS
                     MSLACLPGCIDAKHPKAYPDATAGEYLDERLREIGLKK"
     gene            complement(2921..>3377)
                     /locus_tag="VKQ52_08825"
     CDS             complement(2921..>3377)
                     /locus_tag="VKQ52_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HLZ87331.1"
                     /translation="LLQSPAYKKKKFQYEVKWGIDMQSEHERYLVEKHFRKPVIVTGY
                     PAAIKAFYMRQNEDGKTVAAMDILVPGIGELVGGSQREERLDKLEQRMRDMQIPVEEM
                     WWYLDTRRFGSVPHAGFGLGFERMMLFVTGMTNIRDVIAFPRAPKSAEF"
BASE COUNT          667 a          833 c         1078 g          799 t
ORIGIN      
        1 gggtgccgga gatgcgacgg ataagccagg tcagcagggc gatgaccggg atgaccagga
       61 agaggaagaa cccgctccag gccaggaagt tctgccagaa accttccagg acgtaccctt
      121 tcagatgcag cagaccgtcg ccgcggaagg ccaggccgat caataccatg atcagggaga
      181 aagcgataat gccggcgatg aacaggaaga aggccttgaa gaggacgccg acggcatggc
      241 caaacccggt gccgctctta cggatggcgg ggcctgcttc agccgcccag gcctgggtgc
      301 tttgtttgac ctggctgccc acctgttgaa agctctcccg catctcggtg cccatctcct
      361 ttgcccggcc cttgaagctt tccagatcgc ttttaatggt gttctttata gactccaggt
      421 cgaccttctc cccccgcatt tccagttttt cagatgcggt gacggcttcg gggagtacga
      481 tccacaacac gatataggtg ataaagaggg ttccgccgaa tccgccggtg aagaattccg
      541 gaccgcccca acgatggaac caccagacgt tgcgcattat cgaggtaagc acgctgagga
      601 tcaggggcag ggcgaagatc agccggggga tccacacgtc gatgtggaag taggcggcca
      661 ggccgctggc tacgccgccg atgatccggt cgtcggaatt gcggtagagg cgtttgcgga
      721 cgtttaccgg cagggagcgg ggcggcagga cgatccagag gataatatag aggagtacgc
      781 cgctacccca gccgagcgtc atgagtacga agatgatccg gacgatggcg gggtccaggc
      841 gcaggtagtt ggccaggccg gcgcagacgc ccccgaggat cttgtcgttc ccggcgcggt
      901 acaggcggcg gtgttcatcg ccggcgaact gccagttcgg ttgggaagcg ccggattgct
      961 gctggccggc ggtgctgccg gcgggtgcgg cttcagcggt gctctcgcct tcgaactctt
     1021 cggggcggcc catgctggcg atgatggtgt tgacatcggc gtcagtgatg caggcagcgc
     1081 cttttttcag ggtttcggag aagagttctg caaaccggtt ctcgatatcg ctgatgatct
     1141 cctccttccc ttcctcgttt gcgaaatacc ttcgcaggct ttcgatatac tgctgcagca
     1201 tctcataggc ggtctcttcg atcgggatga cccgactatg aaagtttatg ttaatgatct
     1261 ttttcattgt tagtgtattt ggaggttgag ttgtggcaat tttccggggt tagtgtttat
     1321 tgttggtgag tgcgtggacc gcatttgcca attcggtcca ggtggcttcc agttctccgt
     1381 agaaggcctc cccttttggt gtgagggaga aatatttgcg tggagggccg gagttgcttt
     1441 ctacccaacg ataggttagc atatctgcat tttttagccg ggtcagcaga gggtaaaggg
     1501 tgccttcgag gatattcagg ttcgcgcttt tcatttcttc cacaatatcc gaggggtagg
     1561 cttcaccgcg cctgattacg gagagaatgc aaaactccag gattcctttt cgcatctggc
     1621 tctgggtgtt ctcgatattc atacgctgaa gattttgtac gatgtaaaga tatgttgctt
     1681 tttagtacta tgcaacacac agtacctttt taaactcaga aacctacata gaaaggtaga
     1741 tggaggccat tgaatggcag cggattacgc gcgaaaaggc gggcaaaaaa aatatttttg
     1801 agaagggacg ggtggagaaa aaacggggat aagtggcggc cgctgccggg gtcaggggcg
     1861 tcagcggccg gggtaaacgg ggggccaacg gttggtcggg aacagatcag gggtgccggc
     1921 ggttggccgg agactatttt ttcaggccga tctcgcgcag gcgctcatcc aggtattccc
     1981 ctgcggtggc atcggggtag gcttttgggt gtttggcgtc gatgcagccg ggcaggcagg
     2041 cgaggctcat gctgcttttt ggatgcagga agaaggggat ggagaaacgg gggctgcccc
     2101 agtgttcgcg gggtgggttc accacgcggt gggtggtgga tttgagttta ttattggtca
     2161 gccgttggag catatccccc acgttgacca cgatctggtc gggaagggaa gtgacgggca
     2221 cccattcgtt ctttttggag aggatctcga gcccgtcggc ggaggctccg accagcagcg
     2281 tgatgaggtt gatgtcctcg tgctgttcgg cgcggatggc ggatcttggc tcttctttta
     2341 tcggcgggta gtggatggcc cgcaggatcg aattcccgtt gtggatatag gggtcgaagt
     2401 agttctcatc gagtccaaga tacagcgcga tcgcccggag cagctgggtg ccggaggtct
     2461 cgaaggaccg gtaggccttt gagaacagct tgttgaagtc cggcaattcg gcgacctgta
     2521 cgttatcggg atattcggcc ttgatggggt cgttgtcttc caccgtctga ccgaactgga
     2581 agaattcctt gagatcgggt gcggtgctgc ccttggcatg ttctttcccg aaggaggtgt
     2641 agccacgctg accggcgagg ccggcgatct cgtatttcct ttttatgtcc tggggttggg
     2701 agaagaaggc ctgggtattg ctgtagagtc ccgctaccgt cgcgtcgggg atgccatggt
     2761 tcttcactgc gacgaagcct acttcttcat atgcctttcc gagtgcggat acaaaggcgg
     2821 ctttctgctg gggtgttccc gaggtgaatt cggacaggtc aacgactgga atggccatgg
     2881 cattacattt agaggacatt aaagatagac aaaattcgtg tcaaaactcc gcgcttttcg
     2941 gggcacgggg gaaggcgatg acatcgcgga tattggtcat ccctgtaacg aaaagcatca
     3001 tgcgctcaaa ccccaggccg aagccggcgt gcggcacgct tccgaagcgg cgggtatcga
     3061 gataccacca catctcttcc acggggatct gcatgtcccg catgcgttgt tcgagcttgt
     3121 cgagccgttc ttcgcgctgg gacccgccga ccagttcgcc gatgcctggg acgaggatgt
     3181 ccatggcggc gacagtcttt ccgtcctcgt tctggcgcat gtaaaaggcc tttatggccg
     3241 cgggataacc ggtgacgatg acgggtttcc tgaaatgttt ttccacgagg tagcgttcgt
     3301 gttcgctttg catatcgatg ccccatttta cctcgtactg gaatttcttc tttttatagg
     3361 cgggtgactg aagaagg
//
LOCUS       DATGFG010000477         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_821735, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000477 DATGFG010000000
VERSION     DATGFG010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821735"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VKQ52_08830"
     CDS             complement(<1..745)
                     /locus_tag="VKQ52_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87332.1"
                     /translation="MTIMSRHPVVPLFLVLLAVYASATTACAQPILSAGEHAQLEIRQ
                     AGAHSIRITLKPSTYAGDLPFSPALIDRPYPSPALVVKDLSAPVKKQVGGLVVEISPA
                     PLTVTVTTRDGLPVQRLIFGDSALSFALDDAPVLGLGEGGHKPQPGVNWRTAPIEFDR
                     RGRWQDMQPRWQSDAYGSRNPVPLLIGTKGWALFVATPWGQIDLQAAGDGVFIPWQPT
                     PKDTIQQNEHNQGLAQAKGLPPAGSVVPGL"
     gene            complement(779..1765)
                     /locus_tag="VKQ52_08835"
     CDS             complement(779..1765)
                     /locus_tag="VKQ52_08835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLZ87333.1"
                     /translation="MQYRTLGATNIQLSAIGLGCMSMNHAYGQPDDAESIATLEKAIE
                     LGINFWDTADIYANGANEELVSRVLQPNRKKVFIATKFGFLSYADGKLGGFDASPAHA
                     KKAVEDSLRRLKTDVIDLYYAHRVDPNVPVEDTVGAMADLVKEGKVRYLGLSEASPQS
                     IRRAHAVHPISALQSEYSILTRDVEKEVLPLCRELRISLIPFSPLARGLMTNTLDVAT
                     LGEKDFRRTLPRYQGEHADNNRRLSAGFADLAAAKGCTPAQLAIAWVLAQGDNIIPIP
                     GTKRRKYLIDNAGAIDVRLTSDDFRAIEQLLQQYPNIGDRYNEANSRFLEKK"
     gene            1785..2015
                     /locus_tag="VKQ52_08840"
     CDS             1785..2015
                     /locus_tag="VKQ52_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87334.1"
                     /translation="MDDYIINVGKVEEWQMINDVPSLDEVFQRAKKVLVGGGTVALVR
                     EQRDGTSYRFEEFTNLDDFEAYKKNVYKYLPG"
     gene            complement(2205..>2695)
                     /locus_tag="VKQ52_08845"
     CDS             complement(2205..>2695)
                     /locus_tag="VKQ52_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ87335.1"
                     /translation="QVMYRHILAGLQNQGRVTSEGRPVQPRLPDELSSTFPLNILIAE
                     DNLINQQVILYILQKLGYDPVIVENGRQAVDAALNTGYDIILMDLQMPEMDGLEATRL
                     IRQSRLVDQPIIIALTANTMEGDEEECLAAGMNDYIGKPVKLEELLGKLRKWALQRQL
                     SI"
BASE COUNT          566 a          691 c          809 g          629 t
ORIGIN      
        1 acagaccggg aaccaccgag ccggcgggtg gaagtccctt ggcctgggca aggccctggt
       61 tatgttcgtt ctgctggatg gtatcctttg gagtcggttg ccaggggata aagactccgt
      121 caccggcggc ctggaggtcg atctgccccc agggtgttgc cacaaagagc gcccagccct
      181 ttgtgccgat gagcaggggt acgggattac gggaaccata ggcgtcgctc tgccagcggg
      241 gttgcatgtc ctgccatctt ccgcggcggt cgaactcgat cggcgccgtc cgccagttca
      301 caccgggctg cggtttatgg ccgccttcgc caagacccag cacgggtgcg tcatcgaggg
      361 caaaggaaag ggcgctgtcc ccgaagatga gccgttgcac cggcaaccca tcccttgtcg
      421 tgacggtcac ggtcaatggc gcggggctta tctctacgac cagacccccc acctgctttt
      481 tcaccggggc cgaaagatcc tttaccacca gcgccgggct ggggtagggg cggtcgatca
      541 gcgcgggact gaagggaaga tcaccggcat aggtcgacgg cttcagcgtg atgcggatgc
      601 tgtgtgctcc cgcctgccgg atctccagct gcgcgtgctc accggcggat agaatgggtt
      661 gggcacaggc ggtagtggca gatgcataca cggccaggag gaccaggaaa agcggaacga
      721 caggatggcg gctcatgatg gtcatagtgg gggaaatagg ttatgctcgc agcggcggtc
      781 atttcttttc caggaagcgg gaatttgcct cgttataccg atcgccgatg ttgggatatt
      841 gttgcagcaa ctgctctatt gccctgaagt cgtcactggt aaggcggaca tcgatagcgc
      901 ccgcattgtc gataagatat ttccggcgtt tggtgcccgg tatcgggatg atattatcac
      961 cctgcgccaa aacccaggcg atggccaact gggcgggggt gcagcctttt gccgccgcga
     1021 gatcggcaaa acctgcggag agacggcggt tattgtccgc gtgttctccc tggtaacggg
     1081 gcagggttct ccggaagtct ttctcgccaa gcgtggcgac atcgagggta ttggtcatca
     1141 atcccctcgc cagcgggctg aaggggataa gggagatgcg cagttcccgg cataagggca
     1201 acacctcttt ttccacgtcc cgggtcagaa tggaatattc gctttgcaag gcgctgatgg
     1261 ggtgaacggc atgtgcccgc cggatggatt ggggagaggc ttccgagagc cccagatagc
     1321 ggacctttcc ttcttttacg agatcggcca tggcgccgac ggtatcttcc accgggacat
     1381 tgggatcgac gcgatgcgca taataaagat cgatgacatc ggtcttcaga cggcggagac
     1441 tgtcctctac cgcctttttg gcatgggcgg gcgatgcgtc gaagccaccc agtttcccgt
     1501 cggcatagga aaggaagcca aacttcgtgg cgataaagac ctttttccgg ttgggttgca
     1561 ggacacgcga taccagctct tcgttggcgc cattggcata aatatccgcc gtatcccaga
     1621 agttgatccc gagttcgatg gctttttcca gggtagcgat cgattcggcg tcatcgggtt
     1681 gaccataggc atggttcatg ctcatacaac caagaccgat ggcggacaat tgaatgttgg
     1741 ttgctccgag tgttctatat tgcatttctt taattttaaa tagtatggat gattatatta
     1801 taaatgtcgg aaaagtcgag gaatggcaga tgatcaacga tgtcccctcg ctcgatgaag
     1861 tattccagcg ggcaaaaaag gtattggtcg gcggggggac ggtagccctg gtacgggagc
     1921 agcgcgacgg gacatcatat cgttttgaag agtttaccaa tctcgatgat ttcgaggcct
     1981 ataagaagaa tgtttacaaa tatctgcccg gatagttcga ttaccgtaaa taaaaagagg
     2041 ctatccttgc aggacagcct ctcccggcgc ctatgccggc agattatttt ttggagcaac
     2101 ccggttaagc gagattaacc gaaggtttcc atgatttatc tgtagatagc atgaatacct
     2161 cctttttttt ggtgaacggt agatacgaag ttacggaaaa aatatcaaat tgacaactgc
     2221 cgttgcaacg cccacttgcg aagtttgccc agcagctctt ccagctttac gggcttgccg
     2281 atataatcgt tcatcccggc agccaggcat tcttcctcat cgccttccat ggtattggcg
     2341 gttaatgcga tgatgatggg ttgatcgacg agtcttgatt gccggatgag acgggttgct
     2401 tccaggccgt ccatctcggg catctggagg tccatgagaa tgatatcata gcccgtgttc
     2461 agggcggcat ccactgcctg tcttccgttc tccacgataa cgggatcgta gccgagtttc
     2521 tgcaggatat acaggatcac ctgctggttg atgagattgt cttccgcgat caggatgttc
     2581 agcgggaagg tggaggacag ttcatccggc agtcttggct gcaccgggcg tccttcgctg
     2641 gtgacccggc cctggttttg caatcctgcc aggatatggc gatacatcac ctgtt
//
LOCUS       DATGFG010000478         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_856349, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000478 DATGFG010000000
VERSION     DATGFG010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856349"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1070)
                     /locus_tag="VKQ52_08850"
     CDS             complement(57..1070)
                     /locus_tag="VKQ52_08850"
                     /inference="COORDINATES: protein motif:HMM:NF027652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4961 domain-containing protein"
                     /protein_id="HLZ87336.1"
                     /translation="MKKITLPINKKSWSWIWKVAGTVIIIVIISCNLTISTVDQPASA
                     PGGSIVPITLHCTITSNNPQTSALVVGILVPKIWNASGNTTVTFTADQTTGPQAMSLI
                     PPNTPSPYGNGLSWQDDMLATIGHAGNLIPEYEWIAFQSNSQYTIAANTTESVTVNIQ
                     IKVPNNNVLFNMAYVLAESSDGLHSTAYNNPSTSYYGTFFPPTPLRITGSGTLLDFVN
                     PQLSVVVPGSSLDNDIITIPFNANASVNGLSSANQVYLCATGYTHTKDSIKVCEQTAR
                     TRLVSTGQGQWQTNIWPRGFFNLSPTQVLDSMEYYFTDATGATRVGYGGKATPFSFTF
                     SCQ"
     gene            complement(1072..1503)
                     /locus_tag="VKQ52_08855"
     CDS             complement(1072..1503)
                     /locus_tag="VKQ52_08855"
                     /inference="COORDINATES: protein motif:HMM:NF027710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5004 domain-containing protein"
                     /protein_id="HLZ87337.1"
                     /translation="MLRIIKTGCILLMGWLIFSCKVEHLTPPNEPMKNIAGSWGIIHA
                     IRNGTDMTNGFDFTKFRIVFTDSTYAIDSLLPFIVTTRGKWVFDDPQYPFNLIFTPAD
                     SSRSITAPFLFPVTGGVRNIIFTFSPGCPSNSYQYTLQKVN"
     gene            complement(1521..3263)
                     /locus_tag="VKQ52_08860"
     CDS             complement(1521..3263)
                     /locus_tag="VKQ52_08860"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87338.1"
                     /translation="MRKIFSFLTAVVVLFLLATACTKTGYLTATVISNIDSNDVFTDS
                     TYSMNFLNNVYTKVGFAADPKRFSGGAYAAGLDAACDEAEGPNASSSNGFTMFATGTV
                     SPSIVPADAWANSYSMVRAVNQFLAHLYKIPFNAALKAETRAEARFLRAWYYFILLEH
                     YGGVPLIGDSVFTASTPLHMTRRSFRACVNYILSECDSAYAHLPLTQSGANYGRASGG
                     ACLALKSRVLLYAASPLFNNGGFAKGLSGGLDSIVAYPDADPNRWVLAANAAQTLINL
                     NAYSLFVDSIPGKPGYGFQYLFLQRYNTEYIFANMMDPNKYLEVLWDVPSRGGGGGPH
                     PYQELVDAFPMSNGLPITDPASGYDPNNPYQNRDPRLGYTVIHDSSIRPIFNQPPAPV
                     SLYLNANVNPPVASSQDAVYTGTPTGFYIAKMLDTNVTQYGFNQSYRCLPLMRYAEIL
                     LNFAEASNEAYGPSQYVYQAVEAVRQRAGLRPYQLPAGLDQGGMRTAIQNERRLEFAF
                     EGLRFFDVRRWLIAGATQSLDMHGMEVDRSSSGTTYKSFEVRKHNFTNAMYLWPLPLS
                     EIAKSPELEQNPLY"
     gene            3447..>3965
                     /locus_tag="VKQ52_08865"
     CDS             3447..>3965
                     /locus_tag="VKQ52_08865"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HLZ87339.1"
                     /translation="MFPNLGYLLRWLTGWNLSLPVPTFGFFMALSFWAAYAVFTLELR
                     RRQCLTPATAAVPPDIRIGRLMDQLLLFCGLIGFAGALLLAKLEDSYNLWHHPGQWLL
                     RYHGLTYLGGLIFGAITYLVMLKRRGIPLATAADIGSPGMMLAYAIGRIGCHLAGDGD
                     WGILSTSPKPAWL"
BASE COUNT          861 a          988 c         1211 g          905 t
ORIGIN      
        1 gacagcgcga tgacggcggc gagatatttt tttaccattt gctgaagcat agtcatttat
       61 tggcaagaaa aggtgaagga aaagggagtt gctttgccgc catagcccac cctggtggcg
      121 cccgtagcgt cggtgaaata atattccatg ctgtccagga cctgggttgg cgacaggttg
      181 aagaagccgc gaggccatat attggtctgc cattgcccct gcccggtaga gaccagcctg
      241 gtcctggcag tctgttcgca taccttgata ctgtccttcg tatgcgtata accggtcgcg
      301 cacaaataca cctggttggc cgagctgagc ccgttgaccg atgcattcgc gttgaagggg
      361 atcgtgataa tatcattgtc gagcgagctg ccgggcacca ccaccgacag ctgcgggttc
      421 acgaaatcca gcagggtacc gctcccggtg atgcggagcg gagtgggggg aaagaaagtg
      481 ccgtagtaac tggtggacgg attattatag gcggtgctgt gaagaccgtc cgagctctcc
      541 gcgagcacat acgccatatt gaaaaggaca ttattgttgg gcactttgat ctggatattc
      601 acggtgacgg attccgtggt gttggcggcg atggtatatt gggagttgct ctggaaagcg
      661 atccactcat attccgggat caggttaccg gcgtggccga tggtcgccag catgtcatcc
      721 tgccaactca ggccattgcc ataaggcgaa ggcgtattcg gtggaatgag tgacatggct
      781 tgtggaccgg tggtctgatc tgcggtaaag gtcaccgtcg tattgcccga agcattccag
      841 atcttcggca ccaggatgcc gactacgagc gccgaggtct gcgggttgtt ggaggtgata
      901 gtgcaatgca gcgtaatggg cacgatcgac ccgccgggtg cgcttgccgg ctggtcgacg
      961 gtactgatgg tcaggttgca gctgatgatc acgatgatga tcaccgtgcc cgcgactttc
     1021 cagatccagc tccagctttt cttattgatc ggtaaggtaa tttttttcat gttagttgac
     1081 cttttggagg gtatattgat agctgttgga aggacagccg gggctgaagg tgaagatgat
     1141 attgcggaca ccgcccgtca cggggaacag gaagggagcc gtgatgctcc tgctgctgtc
     1201 cgccggcgta aagatgagat tgaagggata ctgcgggtcg tcgaagaccc atttacccct
     1261 ggtggtgacg ataaaaggaa gaaggctgtc gatcgcgtag gtactgtcgg taaagacgat
     1321 cctgaatttg gtgaaatcga acccattggt catatcggtt ccgttccgga tggcgtggat
     1381 gatgccccag gagccggcaa tattcttcat gggctcgttc ggcggggtga gatgttccac
     1441 cttgcaggag aagatgagcc atcccataag gaggatgcag cctgttttga tgatacgaag
     1501 catggcgttg atcgttagtg ttaataaagc ggattttgct ccagttccgg tgatttggcg
     1561 atctcggaca agggcagcgg ccagaggtac atggcattgg tgaaattatg tttccgtacc
     1621 tcgaaggatt tataggtcgt gcccgatgag gagcggtcta cctccatgcc gtgcatgtcg
     1681 aggctttgcg tcgcaccggc gatcagccat cgtcggacgt cgaagaagcg tagcccttcg
     1741 aaagcgaatt cgagccttcg ttcattttgg atcgctgtgc gcatcccgcc ctgatcgaga
     1801 ccggcgggca gctgataagg acgaagaccc gcacgctgac gaacggcttc cacggcctgg
     1861 tagacatatt gcgacgggcc ataggcttcg ttgctggctt cggcgaaatt cagcaagatc
     1921 tccgcatagc gcataagcgg cagacaacgg taggattgat taaaaccata ctgtgtcacg
     1981 ttcgtatcca gcatcttggc gatataaaaa ccggtaggag tgcccgtata caccgcgtcc
     2041 tggctgctgg ccacgggcgg attgacattc gcattcagat agaggctcac aggcgccgga
     2101 ggttggttga agatcggccg gatggacgaa tcatgaatga ccgtataacc aagccgcgga
     2161 tccctgtttt gataggggtt gttggggtca tagccggaag caggatcggt tatgggcaac
     2221 ccgttgctca tcgggaaggc atctaccaac tcctggtaag gatgcggacc gccgccgcca
     2281 ccgcgggatg gcacatccca caatacctcc aggtatttgt tgggatccat catattggcg
     2341 aagatgtatt cggtgttata ccgttgcagg aagagatatt ggaagccata gcccggcttt
     2401 ccgggtatac tatccacgaa caggctatac gcattgagat tgatcagggt ttgggccgca
     2461 ttggccgcaa gtacccatcg gttggggtcg gcgtcgggat aggccacgat ggaatccagt
     2521 ccgccggaca gtcccttggc aaaaccgccg ttgttgaaaa gcgggctggc ggcgtaaaga
     2581 aggacgcggg acttcagcgc gagacaggcg ccgccgctgg cacggccata gttggcgccg
     2641 ctttgggtca ggggcagatg ggcataggcc gaatcgcatt cggaaaggat atagttgacg
     2701 caggcccgga aggagcgacg ggtcatatgc agcggtgtgg atgcggtgaa aacggagtcg
     2761 ccgatcagcg gaacgccgcc atagtgttcc agcaggataa aatagtacca ggcgcgcaag
     2821 aaccgggcct ccgcccttgt ctcggccttc agcgcggcgt tgaagggtat cttatagaga
     2881 tgggcgagaa actggttgac ggcgcggacc atggagtagg agttggccca ggcgtcggcg
     2941 gggacgatgg aggggctgac cgtaccggtg gcgaacatcg taaacccgtt ggagctggaa
     3001 gcattgggac cctccgcctc gtcgcaggca gcatccaggc ccgcggcata cgccccaccg
     3061 ctaaagcgct tgggatcagc ggcaaaaccc accttggtat agacgttatt cagaaaattc
     3121 atcgagtagg tgctgtcggt aaaaacgtcg ttggagtcga tgttggaaat tacggtggcg
     3181 gtgagatacc ccgtttttgt acatgccgtc gccaaaagga agaggacgac aacggcggtc
     3241 aggaaggaaa atatttttct cataagcaag taatcgtttg ttctcggctt tggtttccgg
     3301 cttcggctcg caccggcctg ataaaagtag aacctgaaaa acgatttagc aaatttttaa
     3361 ggtacttttt tgcttaatta tttatttaca cagtcaaaat aatgccattt tgttgtttta
     3421 gtaaaattgt accatcttgt gagatcatgt ttcctaacct cggctatctg ttgcgctggt
     3481 tgacgggctg gaacctaagc ctgccggtcc ccactttcgg gttcttcatg gctttgtcgt
     3541 tttgggcggc ctacgccgtc ttcaccctcg agctgcgccg caggcagtgc ctcacccctg
     3601 ccaccgccgc ggtccccccc gacatccgca tcggccgcct catggatcaa ctgctgctgt
     3661 tttgcggact catcggcttc gccggcgccc tcctgctggc caaactggaa gacagctata
     3721 acttgtggca tcaccccgga caatggctgc tccgctatca cgggctcacg tatttaggcg
     3781 gactcatatt cggcgcgata acctacctgg taatgctcaa gcgtcgcggc atcccactgg
     3841 cgacagcggc ggatatcggc tccccgggga tgatgctggc ctatgccatc ggaaggatag
     3901 gttgtcatct ggccggtgat ggcgactggg gcatcttgtc gacttcgcca aaaccagcgt
     3961 ggctg
//
LOCUS       DATGFG010000479         7124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1493651, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000479 DATGFG010000000
VERSION     DATGFG010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493651"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VKQ52_08870"
     CDS             complement(<1..1290)
                     /locus_tag="VKQ52_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HLZ87340.1"
                     /translation="MDQHQSLWAFMHQQSAKLLQQTIQHIGLTFISLLIAILIGLPLG
                     IYIARNKKAAGIVLGAAGVLQTIPSIALLGFLIPVLGIGPIPAITALFLYALLPVIRN
                     TYTGINGVDPGVKEAALAMGMTATQRLFRVDLVLAFPVIMAGIRTATVINVGVGTLAA
                     YIAAGGLGEFIFGGIALNNTNMILAGAIPAALLAILLDFLLSLVQRTGSRRRRAVLGL
                     LPIGLLLFSSFYVLPIGDTRLLAGFTPEFMGRRDGDIGLREVYGLKIRTVVISDAVMY
                     KAIYDHKLDVISGYSTDGRLEAYGLVVLDDDKKIFPPYYAAPVVRNEVLRQYPELAPV
                     LNKLAGRINDSVMTLLNYRAEYLNQPPDRIARDFLVSQGLYQPPRNGKKGIIRLGSKI
                     FPEQYILTDMYAMLIRGFTDLEVESKTGLGGTKICFEA"
     gene            complement(1290..2045)
                     /locus_tag="VKQ52_08875"
     CDS             complement(1290..2045)
                     /locus_tag="VKQ52_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ87341.1"
                     /translation="MIVVKNITRNFGAHKAVDNISFTVEKGENIVFLGTSGSGKTTLL
                     KMINRLLEPDSGEILIDGRPITGQRPEDLRRNIGYVFQNNGLFPHYTIAENIAIVPRL
                     LGWGRDRIAQRVTTLMEKLRLSPAQYRDAYPSELSGGQQQRVGIARALAADAPILLMD
                     EPFGALDPITRGKVKKDFKELDELRAKTIVLVTHDIQEAFELADRIFLMDGGRIMQEG
                     TAEDLLDRPANQYVKDFFEDQRYIYDHFKNKKS"
     gene            complement(2042..3409)
                     /locus_tag="VKQ52_08880"
     CDS             complement(2042..3409)
                     /locus_tag="VKQ52_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HLZ87342.1"
                     /translation="MVKYDAIIIGSGQAANPLAKKLAAAGWKTALIEKKWIGGCCINV
                     GCTPTKTLIASGRVAYLVKRSADFGIHTTGVSVNIEEVIRRKNAHVLTARESTAKSLL
                     ETYNLDVLFGNAVFTGEKEITVTNEDGSVAELTAEKIFINTGTRPVIPSLPGLADIPY
                     LTSDTILDLLEIPSHLAIIGGSYIALEFGQLYRRLGSEVTIIENNSRFLSKEDEDIAM
                     AIKTILEEDGIRIHTGTAVQRVAKEEKGIRLESSAGAITASHVLVATGRAADTSALNT
                     AGAGIALDRHGFIIVNDRLETNVAGIYALGDVKGGPQFTHISYNDHLLVYKNLIEKAD
                     CSTAGRLPVYCLFTDPELGRIGLTEEAARDKGMNIKVATLPASHIARAWENDETRGML
                     KAIVNADDGTIVGAAMLSVAGGELMSILQMAMMGGITYDKLRDAIIAHPTFAESLNNL
                     FAKLQ"
     gene            3562..3858
                     /locus_tag="VKQ52_08885"
     CDS             3562..3858
                     /locus_tag="VKQ52_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87343.1"
                     /translation="MKFEEFKTSLSHGQPPSGITPSLTALWYAGKGDWEQAHNIAQDL
                     PTRDGSWVHAYLHRVEGDQGNASYWYNRADRKMPALTLEQEWEAITKAMLASGE"
     gene            complement(3827..4516)
                     /locus_tag="VKQ52_08890"
     CDS             complement(3827..4516)
                     /locus_tag="VKQ52_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLZ87344.1"
                     /translation="MSLTAILKKHRADYHPVVPFDAGRDRLYTFDFTTNNTHLSADDI
                     ADTDRFAAWINRTLQQNKARYGIGGYGEHRTLYARSKHFDQGAEPRRFHLGVDIWGPA
                     GTKVMAPLNGIVHSFAFNNNDSDYGATILLTHNLDGVGFHTLYGHLSLNSLKNLYEGK
                     NVTKGEVIAEFGMRFENGNWPPHLHFQLIADMQGWKGDYPGVCRFSERQQWLDNCPDP
                     EIILRMQASPL"
     gene            complement(4528..>7124)
                     /locus_tag="VKQ52_08895"
     CDS             complement(4528..>7124)
                     /locus_tag="VKQ52_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLZ87345.1"
                     /translation="GTNIANFRRQMDNIGFCFDWSREFRTSEPAYYKWTQWIFLQLFD
                     SFYDKKQNKALPIEWLVRYFEKDGNVHHPCPGDASTRFTAADWTAFDHRRRQEILMNY
                     RLAYCGYGEVNWCEALGTVLANDEVINGVSERGGYPVVKKKLRQWYLRITDYADRLLE
                     GLGTVDFSDAMKEMQTNWIGKSFGAEIDFAIAGQPAGTNLRVYTTRPDTLFGVDFMVI
                     APEHELLETITSAGQRPAVEEYLGYVKSRSERERMAEKKITGCFTGAYAIHPFSGLHI
                     PIWTSEYVLAGYGTGAIMAVPCGDERDFKFARFFDLPITNIIGDHFNGEEANPTKEAV
                     LQNSGFLDGLVMKDAIVIAIKKLEDKGIGTRKVNYRMRDAAFSRQRYWGEPFPIVWKN
                     GIAEALPEMALPLELPYVEKYGPGPEGEGPLANVPEWTAKHLETNTMPGYAGSSWYFL
                     RYMDPQNEEQFCSRAASDYWGQVDLYIGGTEHAVGHLLYSRMWTKFLFDRGYIGHDEP
                     FKRLVNQGMIQGSSRFVYRLELKGRPVEMKGQGKAASPGSDTPVFVSGGIVALQGTPE
                     FHDLVTLAVRGIFDDPSIELESIAVSALHVDVNIVDGVELDIEAFKKWRAEYADAEFI
                     LEEGKYICGTEVEKMSKRLYNTVNPDDLVNRYGADTFRMYEMFLGPVEQSKPWDTKGI
                     EGVHRFIRKFWRLFFDETKGKVWTTQKATDQEWKVLYRTIKKVEEATERFSFNTGVSA
                     LMIGVNELTDLKCHKKEVLEKLIVALTPYAPHFAEEIWQVLGNAGSVLDAAFPVVEEK
                     YLVESTKNYPIAINGKTRHELNIALDATQQEVEAIVLADDVVKKWLDGNPPKKVVYVK
                     NRMINVVI"
BASE COUNT         1475 a         2044 c         2021 g         1584 t
ORIGIN      
        1 cgcttcgaaa catatttttg ttccgccaag accggtttta ctctccactt ccaggtcggt
       61 aaaaccccgg atgagcatgg catacatatc cgtcaggata tattgttcgg ggaagatctt
      121 tgaacccaga cggatgatcc cctttttccc gttgcggggc ggctggtaca gcccctggga
      181 tacgagaaaa tcccgggcga tccgatcagg aggctggttg agatactcgg cccggtagtt
      241 gagcagggtc atgaccgagt cgttgatccg gcccgctaat ttattcagca cgggagccaa
      301 ttcgggatac tggcggagga cctcgttccg caccacgggc gcagcataat aaggcggaaa
      361 gatctttttg tcgtcgtcca gcacgacaag cccataggcc tccagcctcc catccgtact
      421 atagccgctg atcacatcca gtttatgatc atagatggct ttatacatca cggcatcgct
      481 gatgaccacg gtacggatct ttagtccata cacctcccgc aggccgatat cgccatcgcg
      541 acggcccatg aactccggcg taaaccccgc cagcagccgg gtgtcgccga tggggagcac
      601 ataaaaagag gagaatagca gtaaccctat cggcagcagt ccgaggacgg ccctgcgccg
      661 ccggctgccc gtgcgctgta ccagtgaaag caggaaatcc aggaggatgg ccagtaaggc
      721 ggcgggaatg gcgccggcca ggatcatatt ggtattgttg agggcgatgc cgccgaagat
      781 gaactccccg agcccgcccg ctgcgatata ggcggcgagc gtgccgacgc cgacattgat
      841 caccgtagcc gtacggatac cggccatgat cacgggaaag gccaggacga gatcgaccct
      901 gaacaaccgt tgggtagccg tcatccccat cgccagcgcc gcctctttta cgccggggtc
      961 caccccgttg atacccgtat aggtattgcg gataacgggc agcagggcat agagaaagag
     1021 tgcggtgatc gcgggtatgg ggccgatgcc cagcacgggg ataagaaagc ccagcagggc
     1081 gatgctgggt atggtctgca ggacacccgc ggcgccgagc acgatccccg ccgctttttt
     1141 attccgggcg atatagatgc cgagcggcag accgataagg atggcgataa gcagggaaat
     1201 aaaggtcaga ccgatgtgct ggatcgtctg ctgcagcagt ttggcggatt gctgatgcat
     1261 aaacgcccaa aggctctggt gttggtccat cagctttttt tattcttgaa gtgatcatat
     1321 atgtaccgct ggtcttcgaa aaaatccttc acatattgat tggccggccg atcaagcagg
     1381 tcttcggctg tcccttcctg catgatccgg ccgccatcca tcagaaagat ccggtcggcc
     1441 agctcaaagg cctcctgtat atcatgggta accaggacga tcgtctttgc ccgcagctcg
     1501 tcgagctcct tgaaatcctt cttcaccttt ccgcgggtga tgggatcgag ggcgccaaag
     1561 ggttcatcca tcaggagaat gggcgcgtcg gcagccaggg cccgggcgat gcccacgcgc
     1621 tgttgctggc cgccactgag ctcgctggga taagcgtccc ggtattgcgc cggtgacaac
     1681 cgcagctttt ccatcagcgt cgtgacacgc tgcgctatcc ggtcccgccc ccagcccagc
     1741 agacggggga cgatggcgat attctcggcg atggtataat gcgggaagag accattgttc
     1801 tgaaagacgt aaccgatgtt ccggcggaga tcttcgggcc gctgaccggt gatcgggcga
     1861 ccatcgatca ggatctcccc cgagtcgggt tcgagaagac ggttgatcat cttcagcagg
     1921 gtcgtcttgc cggaaccgct tgttcccagg aagacgatat tctccccctt ttcaacggta
     1981 aaggagatat tgtcaacggc cttgtgcgcg ccgaagttcc gggtgatatt ttttactacg
     2041 atcattgcaa ttttgcaaac aggttattca gggattccgc aaaggtcgga tgagcgatta
     2101 tcgcatccct cagcttgtca taggtgatcc cacccatcat cgccatctgc aggatgctca
     2161 tcagctctcc tcccgcgacg gataacatcg ccgctccgac gatggtcccg tcatccgcat
     2221 tgacgatcgc cttcagcatc ccccgggtct cgtcgttctc ccaggcacgg gcaatgtgcg
     2281 aagcgggcag ggtcgccact tttatattca tgcccttgtc ccttgccgcc tcctcggtga
     2341 gcccgatcct gcccaattcg ggatcggtga aaagacaata caccggcagc cgccccgccg
     2401 tgctgcaatc ggccttttcg atcaggttct tgtacacgag cagatggtcg ttgtaggata
     2461 tatgggtgaa ttgcggaccg cccttgacat cgccaagagc atagataccc gcaacattcg
     2521 tctccagccg gtcattgaca ataataaacc catgacgatc gagcgcgata ccggcgccgg
     2581 cggtgttgag agcggaagta tcggccgccc gccccgtagc caccagcaca tgggaagccg
     2641 tgatggcccc cgcggaactc tccagccgga tgcccttctc ctccttcgcc acgcgttgca
     2701 ccgcggtacc cgtgtggata cggatgccat cctcttcgag gatcgtcttt atcgccatcg
     2761 cgatatcctc atcctccttg gaaagaaagc ggctgttgtt ctcgatgatg gtcacctcgc
     2821 ttccgagacg acgatagagc tggccgaatt cgagcgcgat atagctgccg ccgatgatcg
     2881 ccaggtggga ggggatctcc agcagatcga ggatcgtgtc ggaggtgaga tagggaatgt
     2941 cggccagccc gggtaaagag gggatcaccg gtctcgtccc ggtgttgata aagatctttt
     3001 ccgccgttag ttccgcaacg gatccatcct cgtttgtcac cgtgatctct ttttccccgg
     3061 tgaacaccgc attgccgaac agcacatcga gattatacgt ttccagcagg ctcttcgccg
     3121 ttgattcgcg ggcggtcagc acatgcgcat tcttccggcg gatcacttct tcgatattga
     3181 cggaaacgcc ggtggtatga atgccaaaat cggcgcttct ttttaccagg taggcgacgc
     3241 gacccgaggc gataagagtc ttcgttgggg tacacccgac attgatacag catccgccga
     3301 tccatttctt ttcgatcagg gccgtcttcc agcccgcggc cgcgagcttt tttgccaaag
     3361 ggttggcggc ctgacccgag ccgatgatga tggcgtcata cttgaccatt gctgatgaga
     3421 ttgtcctaaa aataagcaat ttttagacca tccggcggct tccttccatc taccggcgcg
     3481 gcgggttgtc catccgcggt aagcccccta cctttgatgg gtgaaacagg gggaaaacca
     3541 cttatgcaaa acggaacaac tatgaaattc gaggaattca aaaccagcct gagtcatggc
     3601 cagccgcctt cgggcattac tccgagcctt acggcgttgt ggtatgcggg taagggtgat
     3661 tgggagcagg cgcataacat cgcacaggat cttcccaccc gcgatgggtc ctgggtacac
     3721 gcttatctgc accgggtgga aggagatcag gggaatgcca gctattggta caaccgggct
     3781 gaccggaaga tgcccgccct gaccctggag caggaatggg aagccatcac aaaggcgatg
     3841 cttgcatccg gagaatgatc tccgggtcgg gacagttgtc cagccattgc tgccgttcgg
     3901 agaaacggca aacgccgggg tagtctccct tccagccctg catatcggcg atcagctgaa
     3961 aatggagatg gggcggccag ttcccgttct cgaaccgcat cccgaactcc gcgatcacct
     4021 ccccctttgt gacattcttt ccttcataaa gattcttcag ggagttgaga ctgagatgac
     4081 catataatgt gtgaaaaccg acgccatcga ggttatgggt gagaaggata gtggcgccgt
     4141 aatcactgtc attattgttg aaggcaaaac tgtggacgat gccgttcagc ggcgccatga
     4201 cctttgttcc cgcgggtccc cagatatcga cgccaagatg aaaccggcgg ggttcggcgc
     4261 cctggtcgaa atgtttactc cgggcataga gggtccggtg ctcgccatag ccgccgatgc
     4321 cgtagcgggc cttgttctgc tgcaatgtcc ggttgatcca ggcggcgaag cgatcggtat
     4381 cggcgatatc atccgccgaa agatgggtat tgtttgttgt aaaatcgaag gtgtatagcc
     4441 tgtccctgcc cgcatcgaag gggaccaccg ggtgataatc ggcccggtgc tttttcagta
     4501 tggcggttag ggacatggtt tgcgcgctta aataacgaca ttgatcatcc tgttctttac
     4561 atacaccacc tttttcgggg gattcccatc cagccatttt tttacgacgt catcggccag
     4621 tacgatggct tccacttcct gctgggtcgc atcgagggcg atattgagct catgacgggt
     4681 cttgccgttg atcgcgatgg gatagttctt tgtcgattcg acgagatatt tttcctccac
     4741 gaccgggaaa gccgcatcga gcacggaccc ggcattaccc aacacctgcc agatctcctc
     4801 ggcaaaatga ggggcatagg gcgtcagggc cacgatcagt ttctccaaca cctccttctt
     4861 atgacatttc agatcggtga gttcgtttac gccgatcatc agtgcgctga cgccggtgtt
     4921 gaacgaaaag cgttcggtcg cttcttccac ctttttgatc gtccggtaaa ggaccttcca
     4981 ctcctggtcc gttgccttct gcgtcgtcca gaccttgcct tttgtctcat cgaagaagag
     5041 ccgccagaac ttgcggataa accggtggac accttcgata ccctttgtat cccagggttt
     5101 gctttgttcg acggggccga ggaacatctc gtacatccgg aaagtatcgg ctccatatcg
     5161 attgacgaga tcgtcgggat tgacggtgtt gtagagtcgc ttgctcattt tttccacctc
     5221 cgttccgcag atgtacttgc cctcttcgag gatgaattcg gcgtcggcat attcagcgcg
     5281 ccatttttta aacgcctcga tatccagttc taccccatcg acgatgttga catcgacatg
     5341 gagggccgaa acggcgatgg actccagctc aatggaggga tcgtcgaaaa tacctctcac
     5401 ggccagcgta accaggtcat ggaactcagg ggtaccctga agagcgacaa tcccgccgga
     5461 gacaaatacc ggtgtatccg atcccggcga tgccgcctta ccctggccct tcatctccac
     5521 cggccttccc ttcaattcga gccggtagac aaaccggctg ctcccctgga tcatcccctg
     5581 gttgaccagt cgtttgaacg gttcgtcatg accgatatag ccccggtcaa agaggaattt
     5641 ggtccacatc cggctataga gcaggtggcc gacggcatgc tcggtgccgc cgatatagag
     5701 gtctacctgg ccccagtaat cggaggcggc gcggctgcaa aactgctcct cattctgcgg
     5761 gtccatatac cggaggaagt accagctgct gccggcatac ccgggcatgg tattggtctc
     5821 gaggtgtttt gccgtccatt cggggacatt cgccaggggg ccttcgcctt cgggaccggg
     5881 accgtatttc tcgacatacg gcaactccag cgggagcgcc atttccggca gggcctcggc
     5941 aatgccgttc ttccacacga tcggaaaggg ttcgccccag tagcgctggc ggctaaaggc
     6001 ggcgtcgcgc atccggtagt tgaccttccg ggtgccgata cccttatcct ccagtttctt
     6061 tatggcgatg acgatggcgt ccttcatcac caggccgtcg aggaaaccgc tgttctgcaa
     6121 tacggcctct ttggtaggat tggcctcttc gccattgaaa tggtcgccga tgatattggt
     6181 gatgggcagg tcgaaaaagc gggcgaattt gaagtcgcgc tcgtcgccac agggtacggc
     6241 catgatggcg cccgtgccat agcccgccaa tacatattcc gatgtccaga tgggaatatg
     6301 caacccgctg aaaggatgga tggcataggc cccggtaaaa cagccggtga tcttcttctc
     6361 cgccatccgc tcccgctcgc tgcggctctt cacatagccg aggtattcct ccacggccgg
     6421 acgctggccc gccgatgtga tggtttcgag gagctcgtgt tcgggggcga tgaccatgaa
     6481 gtcgacgccg aagagggtat cgggccgggt ggtatagaca cggaggttgg tgccggcagg
     6541 ctgcccggcg atggcaaaat cgatctcggc gccaaagctc ttgccgatcc agttggtctg
     6601 catctccttc atcgcatcgc tgaaatccac cgttccgagg ccctcgagca ggcgatcggc
     6661 atagtcggtg atccggagat accactggcg cagcttcttc ttgacgacgg ggtacccccc
     6721 ccgctcgctg acgccgttga tcacttcgtc attggcaagg acggtgccca gggcctcgca
     6781 ccagttcacc tcgccataac cgcaataggc caggcgatag ttcatcagga tctcctgccg
     6841 acggcgatgg tcaaaggccg tccaatcggc ggcggtgaac cgggtagaag cgtcgcccgg
     6901 acagggatgg tggacattcc catccttctc gaaatagcgc accagccatt cgatcggcag
     6961 ggccttgttc tgttttttgt catagaacga gtcgaacagc tgcaggaaga tccattgggt
     7021 ccacttgtag taagcgggtt ccgaggtccg gaactctctg ctccagtcga aacaaaagcc
     7081 gatgttatcc atctgccggc ggaagttggc gatgttggtc ccgg
//
LOCUS       DATGFG010000480         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_936063, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000480 DATGFG010000000
VERSION     DATGFG010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936063"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..803
                     /locus_tag="VKQ52_08900"
     CDS             96..803
                     /locus_tag="VKQ52_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87346.1"
                     /translation="MKLKLTPLYILSFLFLTFFVMELHEWAHTAAAALVGGGWGPRGF
                     DHWGFESNATVSNGQRAVATLAGPLVNILLLWVGWGKMGNRDSLTDQSFGCSLVLACL
                     PMAMLKAAVTGGGDVTIGLKLLFTSVDATHHHLIATCGLIIVIIACVPPLIRLFILLP
                     SWLAKFVFFPIFLVLPSYLHHLIVHDLLNGWLTRFENDESLAYAWVIFWTAAIFGGWL
                     FTRRKMENLLVDRELPL"
     gene            complement(826..2001)
                     /locus_tag="VKQ52_08905"
     CDS             complement(826..2001)
                     /locus_tag="VKQ52_08905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ87347.1"
                     /translation="MTLSVIIVNYQVKYFLELCLHSVEKAVRGLDAEIIVVDNHSGDG
                     SCDYLQPRFPGVKFLANKENKGFGRANNQALEQARGQYILFLNPDTILAEDSVTRCLS
                     FLAATSGIGGLGVRMIDGGGRFLKESRRGFPTPWAGFCRLTGLSTLFPHNRLFSRYYL
                     GWLPADKPHPAPVLSGACLWVSRAILTEVGGFDEQFFLYAEDIDLSFRIEKAGYKNYY
                     FPGTTIVHFKGESTSKDLRYVRQFYQAMRQFRRKHINRGLPALFDPFIQAAIRVRAGI
                     AAMGRLAGSGPGPDRQGALRTLLRGDAQETGRVRALLSASGERMIVETEQEADELLFC
                     EGPGFSFLESISFLESITQPGRTGRHNPRVLFHAGGSGSAVGSPDRNGRGEALVLPG"
     gene            2075..2383
                     /locus_tag="VKQ52_08910"
     CDS             2075..2383
                     /locus_tag="VKQ52_08910"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ87348.1"
                     /translation="MTLTLKQIEKISKALGDTNRLKILHYIAGKGGCGECSGLQDVVG
                     LAQPSISHHVKILVESGLIEGEKEGRNYSYTLNSVVLNQYLAALRQLETKGAAVPVLL
                     "
     gene            2424..3212
                     /locus_tag="VKQ52_08915"
     CDS             2424..3212
                     /locus_tag="VKQ52_08915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLZ87349.1"
                     /translation="MLKSTLIRAAEAGAAVMQRYFQGEFTISNKEGINNLVTEADHAS
                     EKAIFEVIKTDYPDHFLLSEEAGEIVMDSNYKWIIDPIDGTVNFAHGLPLCCVSIALE
                     QDGEMILGAVYNPFIKEFFFAQRGYGATLNDKKIVVSGETSVAKSCLVTGFPYTYLDQ
                     PNGPLEVFSRLIRGGVPVRRLGSAAMDLCWVASGRFDGFYEHKLNAWDSAAGFLIVEE
                     AGGRVSDFTGGRYSPYQPHIIATNGKIHDELVEVVNGRHSVLIK"
     gene            3221..4258
                     /gene="thiL"
                     /locus_tag="VKQ52_08920"
     CDS             3221..4258
                     /gene="thiL"
                     /locus_tag="VKQ52_08920"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HLZ87350.1"
                     /translation="MSENRTEISSMGEFGLIEHLTANIEIKNASTLVGVGDDAAVIDH
                     FGRQTVITTDLLIEDVHFDLMYTPLQHLGYKSVIVNLSDIYAMNATPTQITVSIGISN
                     RFSVEALDEFYEGVYAACEHYGVDLIGGDTASSKKGFVISVTAIGEVAPDRFVRRGTA
                     QKGDLLCCSGDLGAAYIGLILLEREKKIFLESPGVKADLEGEKYVIGRLLKPEARKDI
                     VEFFQQSEILPTSMMDISDGLSSEILHICRQSGLGCVLHEEKIPIAEETRNAAYKFEL
                     DPTACALSGGEDYELLFTVAQQDYDKLVLNEQISVIGYMTEPEKGSKIITKGGNIFDI
                     TAQGWNAFASK"
     gene            4267..>4791
                     /locus_tag="VKQ52_08925"
     CDS             4267..>4791
                     /locus_tag="VKQ52_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87351.1"
                     /translation="MNRLYSFFLLVFSFFLFACGASQKGFRPDRKYAPEALRKDYMVF
                     RHLLEAWHPSLYWYTPRDSMSAYFDEGYAAIRDSMTEPQFRTLLSFVISKADCGHTSV
                     RASRAYAHYLDTAHLPQFPLILKFWTDTMVVAANLNRRDTVLKRGTVILSINGRTTHE
                     LTDTLFNYIVSDGLN"
BASE COUNT         1036 a         1293 c         1369 g         1093 t
ORIGIN      
        1 ggtaattgaa tttcaatcaa cgccattgag taacccttgt catttggtat gcggcgaaat
       61 taacgttttg gatgatattt ttgttacatt tatttatgaa gctaaaactg accccccttt
      121 atatcctatc cttcctcttc ctgaccttct tcgtgatgga gttgcacgag tgggcgcata
      181 cggcagcggc agccctggtc ggcggaggtt gggggcctcg cgggttcgac cattggggct
      241 ttgaatccaa tgcgacggtc tctaacggtc agcgggcggt ggccaccctg gccggacccc
      301 tggtcaacat cctgctcctg tgggttggct ggggaaaaat gggcaaccgg gatagcctaa
      361 ccgatcagtc ctttggctgc agcctggtcc ttgcctgcct gccgatggcg atgctaaagg
      421 cggccgttac cggcgggggc gatgtgacca ttggtctgaa gctgctgttc acgagtgtgg
      481 acgccacgca tcatcacctg atcgccacct gtggtctgat catcgtcatc atcgcctgtg
      541 tcccgcccct gatacgacta ttcatccttc tccccagttg gctggcgaag tttgtctttt
      601 tccccatttt cctggtcctg ccttcctatt tacatcatct catcgtgcac gatctgctca
      661 acgggtggct gacgagattt gagaacgatg aaagcctggc ctatgcctgg gtaattttct
      721 ggacagcggc catctttggc ggctggttgt ttacccgaag aaagatggaa aatctgctgg
      781 tggatcggga actgcccctg taacagggct ccctttgtaa cgaccttacc ccggaaggac
      841 cagcgcctct cccctgccgt tgcgatcggg gctaccgacg gccgaaccgc tgccgcccgc
      901 atgaaacagg acgcgcgggt tatgccgccc cgtcctaccc ggttgcgtga tgctctccag
      961 gaaagagatg ctctccagaa acgaaaagcc cggcccttcg caaaacagca gctcatccgc
     1021 ctcctgttcg gtttccacga tcattcgttc acccgaagcc gacagcagcg cccgtacgcg
     1081 cccggtctcc tgtgcatcac cccgaagaag ggtgcgaagg gcgccctgcc gatccggccc
     1141 tggtccgctt ccggccagtc ttcccatcgc ggcgatgccc gcccgtacgc ggatagccgc
     1201 ctggatgaaa ggatcgaaca gggcgggtaa tccccggttg atatgcttgc gacgaaactg
     1261 gcgcatggcc tggtagaact gccggacgta gcggaggtct ttgctggtgc tttccccttt
     1321 gaaatggacg atggtcgtcc cggggaaata gtaattttta tacccggctt tttctatccg
     1381 gaaactcagg tcgatgtcct cggcatagag gaagaactgc tcatcaaagc cccctacttc
     1441 cgtcagaatg gcccggctga cccagagaca ggcgcccgag aggaccggcg ccggatgggg
     1501 cttgtccgcc ggcagccatc ccaggtaata ccgggagaac agccggttgt gggggaaaag
     1561 ggtagaaaga ccggtcaggc ggcaaaagcc tgcccagggg gtaggaaagc cccggcggga
     1621 ctctttcagg aaacggccgc cgccatcgat catccggact ccgaggccgc cgatgccgga
     1681 ggtcgccgcc aggaaggaga gacagcgggt gacgctgtct tccgcgagga tcgtgtcggg
     1741 gttgaggaag aggatatact gtccgcgggc ctgttccagg gcctggttat tcgcccggcc
     1801 aaatcccttg ttctccttgt tggccaggaa cttaaccccg ggaaaccggg gttgcagata
     1861 gtcgcagctg ccgtcccccg agtggttgtc gacgacgatg atctcggcat cgagaccccg
     1921 gaccgctttc tccacggaat ggaggcagag ctcgaggaaa tactttacct ggtaattgac
     1981 gatgatgacg gacagtgtca tggcctcgcg aagtaagcat ttttatgcat atatcaccaa
     2041 aaattgatac atttgcattt gtcaatttaa tgtgatgaca ttaacgctca aacaaatcga
     2101 gaagatatcc aaggccctgg gagacacaaa ccggctcaag atcctacact atatagcggg
     2161 caagggtggt tgtggggaat gttcgggttt acaggacgtg gtcggccttg cgcagccttc
     2221 tatttcgcat catgtgaaga tcctggtcga gtccgggttg atcgaggggg agaaggaagg
     2281 aagaaactac agttacacgc taaacagcgt tgtgttgaat caatatcttg ccgcgttgcg
     2341 gcaactggaa acgaaggggg cggccgttcc tgttctgctg tagtggccga gggattatgg
     2401 gcaggctcgc ctatctttgc gccatgctaa aatcaacact tataagggcg gccgaagcgg
     2461 gagctgccgt tatgcaacgg tattttcagg gagaattcac catctccaat aaggaaggca
     2521 tcaataacct ggtaacggaa gcggaccacg cctcggaaaa agcgatcttt gaagttatca
     2581 agaccgacta tcccgatcac tttctcctca gcgaagaggc gggagagatc gtcatggatt
     2641 ccaattacaa gtggatcatc gaccccatcg acgggacggt gaattttgcg catgggcttc
     2701 ccctctgttg tgtgtccatc gccctggagc aggatggcga gatgatcctc ggcgctgtgt
     2761 ataacccctt tatcaaggaa ttctttttcg cccagcgggg atatggcgct accctgaacg
     2821 ataagaagat cgtcgtgagc ggtgagacca gtgtcgccaa atcctgcctg gtaaccggtt
     2881 ttccctatac ctacctggac cagccgaacg gaccgctgga agtattctcc aggctgatcc
     2941 gcggcggggt ccctgtgcgc cgtctggggt ccgcggcgat ggatctctgc tgggtcgcct
     3001 ccggccggtt tgacgggttt tatgagcaca agctaaatgc ctgggacagt gcagccggat
     3061 tcctgatcgt agaagaggca gggggcaggg tctcggattt caccggtggc cggtattccc
     3121 cttatcaacc ccatatcatt gccactaacg gcaagatcca tgatgagctg gtcgaggtgg
     3181 tgaatggccg gcattccgtt ctgatcaaat aacaggaact atgagcgaaa acaggactga
     3241 gatctcctct atgggagaat ttggattgat cgagcatctt acggcgaaca tcgagatcaa
     3301 gaacgcctct accctggttg gggtcgggga tgatgccgcc gttatcgacc attttggccg
     3361 gcagacggtg atcacgaccg atctgctgat cgaggacgtt cacttcgacc ttatgtatac
     3421 gccgctgcag catctgggct acaagtcggt gatcgtcaac ctgagtgata tctatgcgat
     3481 gaatgccaca ccaacgcaga taacggtgag catcggcatc agcaaccggt ttagcgtcga
     3541 agccctcgat gagttttacg aaggggtcta tgccgcctgc gaacactatg gggtcgatct
     3601 tatcggcggc gataccgcct cttccaaaaa ggggtttgtc atttcggtca cggccatcgg
     3661 cgaggttgcc cccgatcgtt ttgtaaggcg cggcaccgcg cagaaaggcg atcttctctg
     3721 ctgttctggc gacctgggag cggcttatat cgggcttatc ctgctcgaac gggagaaaaa
     3781 gatcttcctc gagagccccg gagtgaaggc cgatctggaa ggcgagaaat atgtcatcgg
     3841 ccggctgttg aaacccgagg cccggaagga tatcgttgaa ttcttccagc agtcggagat
     3901 ccttccgaca tcgatgatgg atatcagcga cgggctgagc tccgagatcc tccacatctg
     3961 ccgtcagagc gggcttggct gtgtactcca tgaagaaaag atccctatcg ccgaagagac
     4021 ccgtaacgcg gcctacaagt tcgagctgga cccgacggcc tgcgcgctta gcggcgggga
     4081 ggattatgaa ttattgttca ctgttgccca acaggattat gacaaactgg tgctgaacga
     4141 acagatcagc gtcatcggct atatgacaga accggaaaaa ggaagtaaga tcattacaaa
     4201 gggaggcaat atattcgata taactgcgca gggatggaat gcctttgcct ccaaatgagc
     4261 ttatccatga atcgacttta ttcctttttt cttcttgtat tcagtttttt cctttttgcc
     4321 tgcggggctt cccagaaagg ttttcgtccc gaccggaaat acgcgcctga agcgctgcga
     4381 aaggattata tggtattccg gcatctgctg gaagcctggc atcccagcct gtattggtat
     4441 acgccgaggg acagcatgtc ggcctatttt gacgagggct atgccgccat ccgggattcc
     4501 atgacggagc cgcagttcag gaccctcctg agctttgtga tctcgaaggc cgattgtggt
     4561 catacttcgg tgcgtgcttc cagggcctat gcgcattacc tggatacggc ccatctcccg
     4621 cagtttcccc tgatcctgaa attctggacc gatacgatgg tcgtcgccgc caatctcaac
     4681 cgccgcgaca cggtcctgaa acggggcacg gtgatcctga gcattaacgg gaggacgacc
     4741 catgagctga cggatacgct tttcaactat atcgtctccg atgggctcaa c
//
LOCUS       DATGFG010000481         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1136428, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000481 DATGFG010000000
VERSION     DATGFG010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136428"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VKQ52_08930"
     CDS             complement(<1..880)
                     /locus_tag="VKQ52_08930"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HLZ87352.1"
                     /translation="MEILHVSAECYPVAKAGGLGDVVGALPKYQRDLGHVAKVVMPMY
                     RTKFLAANQWEVVHKGYTHLHNYWFNYTVIKEQTNKLGFDLYLVDIYGLLDREKIYGY
                     GDDVERFTSFQIAVVDWVASWQHQPDVIHVHDHHTALIPFMLKYFFAYQHLQNIATIL
                     TIHNAQYQGQTGMDKTVFIPAWDVWKRGLLEWAGALNPLACGIRCAWKVTTVSPTYLE
                     EMRSQAAGLEALLEYEKGKCSGILNGIDSVVWNPATDRYLENHYDSTAVEEGKQRNKA
                     LLCQQYNLDPKKPLITF"
     gene            1007..>2119
                     /locus_tag="VKQ52_08935"
     CDS             1007..>2119
                     /locus_tag="VKQ52_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ87353.1"
                     /translation="MQQKTKWSQFGTLVTVFFFWGFVAASNDILIPVFKEKLHLEQWQ
                     AQMISVAFYVAYTIGSIIYYLISKFSGGDILNRIGYKNGIAVGLIISALGSLLFYPAA
                     MSASFAFLITGLFIIGLGYSLQQTAANPLAIIMGDPASGSQRLSMAGGINNFGTTIGP
                     LIVSIAIFGSAQVTPGIARVASISSVKGPYLILGAVFILIALIFKFSSLPNKIDLDQT
                     SADATQSHAVVDRGSPLKYPQLVLGMLGIFIYVGVEVSTASNLPLFMREKLHTPTDEI
                     APYVSLYWASLMIGRWTASVGAFGVSKLAGQILSFIMPYLAFGVFMVVNIIAGHDVHP
                     FYIYPVIIIGMIVGNLLSKGNPARQLLIFSLMGITAL"
BASE COUNT          436 a          651 c          502 g          530 t
ORIGIN      
        1 tgaacgtaat aagcggcttc ttcgggtcca ggttatattg ctggcagagc agggccttgt
       61 tccgttgctt tccctcctcc accgcggtgc tgtcatagtg gttctctaaa taccggtccg
      121 tcgccgggtt ccagacgacg gagtcgatcc cgttcaggat accgctgcat ttccccttct
      181 cgtactccag gagtgcctcc agacccgcgg cctggctccg catctcctcc aggtaggtcg
      241 ggctcaccgt cgtcaccttc caggcgcaac ggatgccgca ggccagggga ttcaacgcgc
      301 cggcccattc gagcagcccc cgcttccaga cgtcccaggc aggaatgaac accgttttgt
      361 ccatacccgt ttgaccctgg tattgtgcat tgtgaatggt cagaatagta gcgatgttct
      421 gtaaatgctg gtaggcaaaa aagtatttga gcatgaaagg gatcagtgcc gtgtgatgat
      481 cgtgcacatg gatgacatcc ggctgatgct gccaggaagc gacccagtcc accaccgcga
      541 tctgaaaaga cgtgaaccgc tccacatcat ccccgtaacc atagatcttt tccctgtcaa
      601 gcaatccata gatgtccacc aggtaaaggt caaaacccag cttattcgtc tgctccttga
      661 tgaccgtata attgaaccag taattgtgca gatgcgtata tcccttgtgt actacctccc
      721 attgattcgc cgccaggaat ttcgtccggt acatcggcat caccaccttc gccacgtgtc
      781 ccagatcccg ctggtatttc ggtaatgccc caaccacatc gcccagcccg ccggccttgg
      841 ccacgggata acactctgct gatacgtgaa gtatttccat tcctgttcaa tttagtaagg
      901 ttttcgatta aacggtgtgc caacgatgaa aaaatttttc gcttcggaaa aaggtggtat
      961 tttggtccct tattctgatt aaagcattaa ccacctttgt ctgcttatgc aacaaaaaac
     1021 caaatggtca cagttcggaa cactcgtcac cgtcttcttc ttctggggat tcgtagcagc
     1081 cagtaacgat attctcatcc cggtcttcaa ggaaaagctc cacctggagc aatggcaggc
     1141 acagatgatc tccgtagcct tctatgtcgc ctatacgatc gggtccatta tttattatct
     1201 catttcaaaa ttcagcggcg gggacatcct caaccgcatc ggctataaga atggtatcgc
     1261 cgtgggcctg atcatctccg cccttggctc actgctattc tatccggccg ccatgtccgc
     1321 ctcctttgcc tttctcatca ccggcttatt tatcatcggt ctgggttatt ccctgcagca
     1381 gaccgcggcc aatcccctcg ccatcatcat gggcgatccg gccagcggct cgcaacgcct
     1441 cagcatggcc ggcggtatca ataatttcgg cactaccatc ggtccgctga tcgtcagcat
     1501 cgccatcttt gggtccgccc aggtcactcc cggcatcgcc agggtagcca gcatcagcag
     1561 cgtcaagggg ccctacctca tcctgggcgc cgtctttatc ctcatcgccc tcatcttcaa
     1621 attctcttcc ctgcccaata agatcgatct ggaccagacc tcggcggatg ccacacaatc
     1681 ccatgcggtg gtcgatcgcg gcagccccct caagtacccg cagctggtgc tggggatgct
     1741 gggtattttc atttatgtag gcgtggaagt atccaccgcc agcaacctgc ccttgttcat
     1801 gcgagagaag ctgcacaccc ccaccgatga gatcgcgccc tatgtgtccc tttactgggc
     1861 cagcctgatg atcggccgct ggacggcctc cgtcggcgcc ttcggggtaa gtaagctcgc
     1921 cggtcagatc ctgagcttta taatgcccta cctcgcgttt ggcgtattca tggtcgtcaa
     1981 catcatcgcc ggtcacgatg tccatccttt ctatatctac cccgtcatca tcatcggcat
     2041 gatcgtcggc aacctcctta gcaagggtaa ccctgcgagg cagctgctga tcttctcttt
     2101 gatggggatc acggccctg
//
LOCUS       DATGFG010000482         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1226384, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000482 DATGFG010000000
VERSION     DATGFG010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226384"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VKQ52_08940"
     CDS             complement(<1..932)
                     /locus_tag="VKQ52_08940"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLZ87354.1"
                     /translation="MQTAPAGNRFAGLDHLRALAIIIVFVYHYGLFGHPPGLNEYFGS
                     WGWTGVDLFFVLSGFLIGGQLFAKLSRNQPINYGEFYLKRSLRILPAYFAVLLIYFTI
                     PAFTERSRLPPLWRFLTFTQNFGLDLSQHGAFSHAWSLCIEEQFYLLLPLLIISLTAW
                     RPKNKPLSDPSTPAEKAVWIIPALFLFGFLVRGYIWYGILDTPGQTAFGKTYYRLIYY
                     PTWSRLDGLLAGVALSALYYFKPTTWQRLTRRGNLLALIALLLIGGAWLLTSGDHQYE
                     MQGAIFGYPAIAIAYGALVLAALSPTSFLYRYSTA"
     gene            complement(979..>2391)
                     /gene="thrC"
                     /locus_tag="VKQ52_08945"
     CDS             complement(979..>2391)
                     /gene="thrC"
                     /locus_tag="VKQ52_08945"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLZ87355.1"
                     /translation="RTTVRLIQPTTSMKYYSLNSPGSFVDFKEATIRGQAPDKGLYFP
                     QSIPTLPKYLLTQIDTLSREELAYQVIRPYTGDTLPESELRRIVAETIDFDFPLVPIE
                     PAQTFAASASPDITSRQPQTFAASPASPDIFSLELFHGPTLAFKDVGARFMSRCLGYF
                     VRDNKRPVTVLVATSGDTGGAVASGFYDVEGIRVVILYPSGKVSSVQELQLTTLGKNI
                     TALEVNGSFDDCQQMVKEAFADKTLQQELFLTSANSINVARWLPQQFYYFFAWQQWTD
                     KKNPPVICVPSGNFGNICAGLLAHQSGLPAAHFIAACNANDVVPVFLQTGEYKTKAAK
                     PTLSNAMDVANPSNFVRILELFQKQLPSLKKTLSSVSISDDETVAAIRRLYTDHHYLA
                     DPHGAVGWLALHRYLDEHPGSKGFFLETAHPVKFYDTVEPIIGQQIPLPASVAALLDK
                     QKQSKKIAALYTNLKDFLLS"
BASE COUNT          469 a          482 c          931 g          509 t
ORIGIN      
        1 gcggtggaat accggtagag gaagctcgtg gggctcaatg cggccagtac gagtgcgccg
       61 taggcgatgg cgatggccgg gtaaccgaag atggcgccct gcatttcgta ctggtggtcg
      121 ccgctggtca ggagccaggc cccgccgatg aggagcaggg caatgagggc caggaggttg
      181 ccgcgccggg tgagccgttg ccaggtggta ggtttgaaat aatagagggc tgaaagggcc
      241 acgccggcga gcaggccgtc cagccgggac caggtgggat agtagatcag gcggtagtag
      301 gttttgccaa aggcggtttg tccgggggtg tcgaggatac cgtaccagat ataaccgcgg
      361 acgaggaagc cgaagagaaa gagggcgggg atgatccaga ctgctttttc ggccggtgtg
      421 gaggggtcgg agagcggttt attctttggg cgccaggcgg tgaggctgat gatgagcagg
      481 ggcaggagga gatagaactg ttcttcgatg caaagcgacc aggcatggga aaatgcccca
      541 tgttgggaaa gatcgaggcc aaaattctgg gtgaaggtga gaaaacgcca gaggggtggg
      601 agccggctgc gttcggtgaa ggccgggatg gtgaaataga tcaacaaaac ggcgaagtag
      661 gccgggagga tgcggagcga tcgtttgagg tagaattcgc cgtagttgat gggttggttg
      721 cgggagagtt ttgcgaaaag ttgtccgccg atgaggaagc cggacaggac gaagaagagg
      781 tctacgcccg tccaccccca gctgccgaaa tattcgttca ggcccggggg atggccgaag
      841 aggccgtaat ggtagacgaa tacgatgatg atggccaggg cccggagatg atccaggccg
      901 gcgaagcggt tgccggcggg ggcagtttgc acgggagaga cagtttatgt ggggggtgaa
      961 agatctgttg tgttgtctct aagataacag gaaatctttc agattagtgt acagggcggc
     1021 tattttttta ctttgttttt gtttgtcgag caatgcggcg acggatgcgg gtaacggtat
     1081 ctgctgaccg atgatgggtt ctacggtatc gtagaatttc accgggtgtg cagtttcgag
     1141 gaagaaccct tttgagccgg ggtgttcatc gaggtatcgg tgcaatgcga gccagcctac
     1201 ggccccgtgg gggtcggcga gatagtggtg gtcggtatag aggcggcgga tggcggccac
     1261 ggtctcgtcg tcggagatgc tgacgctgga cagggtcttt tttagtgagg gcaattgctt
     1321 ttggaagagc tcgaggatgc gcacgaagtt gctgggattg gcgacgtcca tggcattgga
     1381 gagggtgggt tttgcggctt ttgttttgta ttccccggtt tgcaggaaga cgggcaccac
     1441 atcgtttgcg ttgcaggcgg cgatgaaatg ggcggcgggg aggccggact ggtgggcgag
     1501 caggcctgcg cagatgttgc cgaagttgcc gctggggacg cagatgacgg gtgggttttt
     1561 cttatccgtc cattgttgcc aggcgaagaa atagtagaac tgttgcggca gccagcgggc
     1621 gacgttgatg gagttggcgg aggtgaggaa gagttcctgt tggagggtct tgtcggcgaa
     1681 ggcttctttt accatctgct ggcagtcgtc gaagctgccg ttcacttcca gggcggtgat
     1741 attctttccg agggtggtga gctggagttc ctgtacgctg ctgaccttgc cggaggggta
     1801 gaggatgacg acccggatgc cttccacgtc atagaagccg ctggcgacgg cgccgccggt
     1861 gtcgccggag gtggcgacga gtacggttac ggggcgtttg ttgtcgcgga caaagtaacc
     1921 caggcagcgg ctcatgaagc gggcgccgac gtctttgaag gcgagggtgg ggccgtggaa
     1981 gagctcgagg gaaaagatat cgggggaggc tggggatgcg gcgaaggttt gcggctggcg
     2041 ggaggtgatg tcgggggagg ctgatgcggc gaaggtttgc gcgggttcga tgggtaccag
     2101 ggggaagtcg aagtcgatgg tttccgccac gatgcggcgc agctcggatt cggggagcgt
     2161 gtcgccggtg tagggccgga tgacctggta ggccagttct tcgcgggaga gggtgtcgat
     2221 ctgcgtgagg agatatttcg ggagggtggg gatggattgg gggaaataga gtcctttatc
     2281 cggggcctgt ccgcggatgg tggcttcttt gaaatcgacg aatgagccgg ggctgttgag
     2341 gctataatac ttcatactgg ttgttggttg gatgagacgg actgttgttc g
//
LOCUS       DATGFG010000483         6337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1199104, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000483 DATGFG010000000
VERSION     DATGFG010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199104"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VKQ52_08950"
     CDS             <1..318
                     /locus_tag="VKQ52_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HLZ87356.1"
                     /translation="MTLVGVFRWMDLTGVAAYHNVIKELFPTLCNSTDTPRLIDDIVR
                     SGGRGVANGRGFYDYTPEEARLWEETFERFSYEIRELALRYPADVVKRRLSGEGEPVD
                     PNQ"
     gene            338..2815
                     /locus_tag="VKQ52_08955"
     CDS             338..2815
                     /locus_tag="VKQ52_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLZ87357.1"
                     /translation="MKRTILQALLGLQLLLCSVAGNAQTYYVDSSAAAGGDGSAAHPW
                     NNILSAVWGTPQPVTADVTVYFRKGTYYFNYAADSIIYLGSTKSGLNGHQFTLAAYPG
                     EQPVFDGSRLTTPYGYLVSIAGASNIRLRGLTFANITNMSAYGVVISGASDSVHIDSC
                     VFRNMLWDSDTAEAKFPTPADYNIAPIIINGSLGGTPAHIFIDSTLFDEIACGATDSL
                     ITTYGTVGTITQTANIDSDIILPQPRYQFYVSTTGSDTTGTGSKVKPWRTPRYALNNA
                     GYSWTTGSAVLVDSNVTVYLRAGTYYLDTSIYIGSNRGQNGKWTMLTHYPGEDATLDG
                     GHLTQKFSCIIIIDSASYIDIRGLNLQNLTDDSTLNVPGSKDTRFGIEIAGNCSHITI
                     DSNDLYNMKWTRDTTKAKNPQPDDVLSAITVLGNSNTPITDLLISENTIHDIVPGYAE
                     GMTLNGNVDSFTISDNEVYDVANIGICAAGNYKWVLTQYPYLLKANNQSRNGLIKDNT
                     VYRCISPVATSAGIYLDGSLNVKVQGNESYNNGTGLSVGNEQDSSSSGGHTIISNNFW
                     SNLGPGMYLGSNNSTSTCSTVVVKYNTVTDDWTINPTLYARANGRYGTSDSAGQGPEI
                     VMQRVQNLTFDENDVYSSSDNVLEFAFSQSNLVFTYNDYYTRDNNPCKAYFFRDTIGT
                     GISFKKDTTFNQYASETKLDTTSYLGGVAYNRSGCGTHGSSVVIPAATRTGARFAEDS
                     ARLYEYPNPVTNGLYLHIQSPAGGAASLELFDLSGRMLLRQDVTLAAGVNDIGWSDVK
                     QQGVKVGFYVLQLITAEGRTTVKVVVQ"
     gene            2906..3898
                     /locus_tag="VKQ52_08960"
     CDS             2906..3898
                     /locus_tag="VKQ52_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLZ87358.1"
                     /translation="MEYRQLGESGLSASAITFGAWAIGGWMWGGTERKDAVAAIRASY
                     DEGVTSIDTAAVYGQGLSEEIVGEALQGIPRDRVQIMTKFGMRWDLTKGGFAFKSKDN
                     DGRDLEIYKYAGRESIIRECEDSLRRLRTDYIDLYQQHWPDVTTPMAETMEALVELQK
                     AGKVRAVGMSNYDAAQMREAEKTIRLASDQVPYSMVRRDIEKELIPYCIEHKKAILAY
                     SPLQRGLLSGKIRPGHHFNEGDNRGDSKYYQGDNLNRINAFLDELRPLAAAKGATLAQ
                     LVIRWTLQRPGITIALVGARDARQAVQNANAIHVRLSAEEIAFIDGRLERLVLE"
     gene            3967..4377
                     /locus_tag="VKQ52_08965"
     CDS             3967..4377
                     /locus_tag="VKQ52_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87359.1"
                     /translation="MIQFHELRIGDIVLVEFDGQRREGEVTGLNAPDKEIQVENEVQE
                     FWYTPEDLYPIPLDDEQLLKFGFEKQDLPDGKVKYLKGPFRILLSGKGNFSDFEMWYR
                     EDRRHMTQPIAVHNLQNHFHGMTKIDLVRVGQEH"
     gene            4769..4885
                     /gene="ykgO"
                     /locus_tag="VKQ52_08970"
     CDS             4769..4885
                     /gene="ykgO"
                     /locus_tag="VKQ52_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HLZ87360.1"
                     /translation="MKVRASIKKRSADCKIVRRKGKLYVINKKNPRFKQRQG"
     gene            4912..5292
                     /gene="rpsM"
                     /locus_tag="VKQ52_08975"
     CDS             4912..5292
                     /gene="rpsM"
                     /locus_tag="VKQ52_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HLZ87361.1"
                     /translation="MARIAGVDLPKNKRGEIGLTYIFGIGRSTAQYILTKAGIGFDKK
                     VHQWNDEELNAIRNIITNEFKVEGALRSEVQMNIKRLLDIACYRGLRHRKGLPVRGQR
                     TRTNSRTRKGKRKTVAGKKKVAKK"
     gene            5498..5902
                     /gene="rpsK"
                     /locus_tag="VKQ52_08980"
     CDS             5498..5902
                     /gene="rpsK"
                     /locus_tag="VKQ52_08980"
                     /inference="COORDINATES: protein motif:HMM:NF003698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HLZ87362.1"
                     /translation="MAKAQANSAKAAAKKRIVKVDVYGDAHITASFNNIIISLTNKNG
                     QVISWSSAGKMGFKGSKKNTPYAAQMASSDAAKTAVDAGLKKVDVYVKGPGSGRESAI
                     RALSNNGIEVVMIKDVTPLPHNGCRPPKKRRV"
     gene            6026..>6337
                     /gene="rpsD"
                     /locus_tag="VKQ52_08985"
     CDS             6026..>6337
                     /gene="rpsD"
                     /locus_tag="VKQ52_08985"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HLZ87363.1"
                     /translation="MARYTGPKTRISRIFGEPILGNGKYLTKNSNPPGQHGGTKKRKA
                     PGEYALQLKEKQKAKYTYGVLERQFRKTFDEANRRKGVTGENLIKLLESRLDNTVYRL
                     GV"
BASE COUNT         1549 a         1481 c         1930 g         1377 t
ORIGIN      
        1 atgacgctgg tcggggtttt ccggtggatg gacctgacgg gcgtagcggc ctatcacaat
       61 gtaataaaag aattgttccc tacactttgc aatagcacgg atactccccg gttgatcgac
      121 gacatcgtcc gatcgggggg ccggggagta gccaatggcc ggggatttta tgactatacg
      181 ccggaggagg cccggttgtg ggaagagacc ttcgagcgat tcagctatga gatcagggaa
      241 cttgcgctcc ggtaccctgc cgacgtcgtg aagagacggc ttagcggcga aggcgagcca
      301 gtggatccga atcaataacc attaaataga ccctcttatg aagcgtacaa ttttacaagc
      361 cctcctgggt ttgcagctac tcctttgcag tgttgccggc aatgcccaaa cctattatgt
      421 ggacagttct gccgccgcgg gcggcgatgg cagcgcggct catccctgga acaatattct
      481 ttccgcggtg tgggggacgc cgcagcccgt taccgccgac gtaacggtat atttccgcaa
      541 aggaacctat tatttcaatt atgcggcgga ctcgatcatc tatctgggct ctacgaagag
      601 cgggctcaat ggccatcagt tcacgctggc tgcttacccg ggggagcagc cggtctttga
      661 cgggtcgagg ctgacgacgc cgtacggcta tcttgtaagc attgccgggg ccagcaatat
      721 ccggctgagg ggattgacct tcgccaatat caccaatatg agtgcctatg gggtggtcat
      781 cagcggcgcg agcgacagcg ttcatatcga cagctgcgtt ttccggaata tgttatggga
      841 cagcgatacg gccgaggcga aattccctac cccggccgat tacaatattg cgcccatcat
      901 tattaatggt tccctgggcg ggacgccggc gcacatcttt atcgacagca cgctgttcga
      961 cgagatcgcc tgtggggcga cggacagcct gatcaccacc tatggcacgg tgggaacgat
     1021 cacccaaacg gccaatatcg attcggatat cattctccct cagccccgtt accagtttta
     1081 tgtgagtacg acggggagcg atacgacggg aacgggttcg aaggtcaaac cctggcggac
     1141 gccgcgatat gcgcttaaca atgcgggtta tagctggacg acgggctcgg ccgttcttgt
     1201 cgactccaac gtcaccgttt atctccgggc cggaacgtat tacctggata ccagtatcta
     1261 tatcgggtcg aatcgcgggc agaacggtaa gtggacgatg ctgacgcatt acccggggga
     1321 ggacgcgacg ctggacggag gtcatctcac ccagaaattc tcctgtatca tcatcatcga
     1381 cagcgccagc tatatcgata tccggggtct caacctgcaa aacctgacgg atgactcgac
     1441 gctgaacgtg ccgggatcga aggatacgcg gttcgggatc gagatcgccg ggaactgttc
     1501 gcatatcacg atcgacagca acgacctgta caatatgaag tggacaaggg acaccacgaa
     1561 ggcgaagaac ccgcaaccgg atgatgtact cagcgcgatc accgtactgg gtaacagcaa
     1621 tacgcctatc accgatcttc ttatcagtga gaatacgatc catgatatcg tccccggcta
     1681 tgccgagggc atgacgctca acggcaatgt ggattctttt accatcagcg ataacgaggt
     1741 gtatgatgtc gcgaatatcg gcatctgtgc ggcggggaat tacaaatggg tactgaccca
     1801 gtacccttat ctgctgaagg ccaataacca gtcccgtaac ggcctgatca aggacaacac
     1861 ggtctaccgg tgtatctcgc ccgtggctac ttcggcgggg atctacctcg atggatcgct
     1921 gaatgtaaag gtgcagggga atgagagcta taacaacggc acgggactgt cggtgggcaa
     1981 tgagcaggat tcgagcagca gcggcggtca tacgatcatc agcaacaact tctggagcaa
     2041 tctcgggccg gggatgtacc tgggcagtaa caactctact tccacctgta gtactgtggt
     2101 ggtgaagtat aataccgtga ccgatgactg gacgatcaat cccactttat atgcccgcgc
     2161 caatggccgc tatggtacat ccgactcggc gggtcagggg ccggagatcg tgatgcagcg
     2221 ggtgcagaat ctgacattcg atgagaacga tgtctattcc tcgtccgaca atgtattgga
     2281 atttgccttt tcccaatcca atctcgtgtt cacgtacaat gactattaca ccagggacaa
     2341 taacccctgc aaggcctatt ttttcaggga tacgatcggc accggtatat ccttcaagaa
     2401 ggatacgacc ttcaaccagt atgcgtccga gacgaagctg gacaccactt cctacctggg
     2461 cggtgtggcg tataaccgca gtggttgcgg aacgcatggc tcgtcggtgg tgatacccgc
     2521 ggcgacgcgc accggggccc ggtttgcgga ggacagtgcg cggttgtatg aatatcccaa
     2581 cccggtgacc aatggactgt atctgcatat ccagagtccg gcgggcggtg cggcgagcct
     2641 ggagctgttc gatctttcgg gccggatgct tttgcggcag gatgtgacgc tggcggcggg
     2701 ggtgaatgat atcggctggt ccgatgtgaa gcagcagggg gtgaaggtgg gattctatgt
     2761 gttgcagctg atcacggcgg aaggccggac gacggtgaag gtggtagtgc agtagttggg
     2821 ggggcgccag gccgggagtt cgctcagaat aaagttagcc aggtccggtt tgtcctgttg
     2881 cctgtatatt cgtcgaaaaa agggtatgga atacagacaa ctgggtgaat cgggcctttc
     2941 cgcttcggcg atcactttcg gggcctgggc tattggcggg tggatgtggg gtggtacgga
     3001 gcggaaggat gcggtggcgg cgatcagggc ctcctatgat gagggtgtga cgtcgataga
     3061 tacggctgcg gtgtatggac aggggcttag cgaggagatc gttggggagg ccctccaggg
     3121 cattccgcgg gacagggtgc agatcatgac caaattcggg atgcgctggg atctaacgaa
     3181 gggcggtttt gccttcaaga gcaaggacaa tgacggcaga gacctggaga tctacaaata
     3241 tgcgggtagg gaaagcatca tcagggaatg cgaggacagt cttcgccggc tacggacgga
     3301 ctatatcgat ctgtatcagc agcactggcc ggatgtgacg acgccgatgg cggagacgat
     3361 ggaggcgctg gtggagctgc agaaggcggg gaaggtccgg gcggttggga tgtccaatta
     3421 tgatgcggcg cagatgcggg aggcggaaaa gacgatccgg ctggcgtctg accaggtgcc
     3481 ttacagcatg gtgcgccggg acatcgagaa ggagttgatc ccttattgta tcgagcataa
     3541 gaaggccatc ctggcgtata gtccgctgca acgggggctc ttatcgggga agatcagacc
     3601 ggggcaccat ttcaatgagg gggataaccg cggggacagc aagtattacc agggggacaa
     3661 cctgaaccgg atcaatgcgt tcctggatga gttgcggccg ttggcggctg cgaagggggc
     3721 gacgctggcc caattggtca tccgctggac cttgcagcgg ccgggtatca ctattgcatt
     3781 ggtgggggcg agggatgcgc ggcaggcggt gcagaatgcg aatgccatcc atgtgcggct
     3841 ttcggcggag gaaattgcgt ttatagacgg ccggttggag cggttggtcc tggagtaggg
     3901 gtaatcttct gttggggatt ttcatgaaaa gccgtaaatt ggaaaataac actaaaacga
     3961 tgtcatatga tccagtttca cgaattacga attggcgata ttgtgctggt cgagtttgat
     4021 ggtcagcgca gggagggtga ggtgacgggt ttgaatgcgc cggacaagga aatacaggtg
     4081 gaaaacgaag tccaggaatt ctggtatact cctgaggacc tgtaccccat acccctggat
     4141 gatgagcaac tgctgaaatt cgggttcgag aaacaggatc tgccggatgg caaggtgaag
     4201 tatctgaagg gccccttccg catacttttg tccggaaaag ggaatttttc cgattttgag
     4261 atgtggtatc gggaggaccg gcggcatatg acgcagccga tcgcggtgca taacctgcag
     4321 aatcatttcc atgggatgac gaagatcgac ctcgtgaggg tggggcagga gcattgaggg
     4381 gggtggatgc gtggattggg ctaaatttta gcatttttgg ccggtccggg cggtgaggtt
     4441 gaaaatattt acctacattt attgataaaa ttgaattttt ttgccagaat tttcataatt
     4501 cggcattata atcctatctt tgcgctccca aaatctgtat ggctaaacaa gcactcatta
     4561 aacaagacgg tatcattctg gaagccttgt ccaatgctat gttcaaagtc aagctggaaa
     4621 atgggcatga gatcctggca accatctcgg ggaaaatgag gatgaattac atacggatat
     4681 tgccggggga taaggtaggg gtggaaatga gcccttatga tctgacgaga ggtagaatta
     4741 tattcagata caaataataa gtattgttat gaaagttcgt gcatccatta aaaagcgcag
     4801 cgccgattgt aagatcgtga ggaggaaggg taagctgtat gttatcaaca agaagaaccc
     4861 ccgtttcaag cagcgtcagg gttaaatgat taaaaactat aaatttagaa tatggctcgt
     4921 attgccggtg tagacttacc aaaaaataaa agaggggaga tcggacttac ctatatcttc
     4981 ggtattggcc gttccaccgc tcagtatatc ctgaccaagg cagggatcgg tttcgacaag
     5041 aaggtgcatc agtggaatga tgaggagttg aacgccatcc gtaacatcat tacgaatgag
     5101 ttcaaggtcg aaggcgcgct tcgttccgag gtgcagatga atatcaagcg tttgctcgat
     5161 atcgcctgtt atcgtgggct gcgtcatcgt aagggtctgc cagtgcgtgg tcagcgtacc
     5221 cgtaccaaca gccgtacgag gaagggtaag cgtaagacgg ttgccggcaa gaagaaggtg
     5281 gccaagaaat agttgataca catgagccgg cggctgtcat tgggcgtcgt cattgggcgt
     5341 cgtcattggg cgtcgcttgg ggggggcagg gtcggctcgt gttgttcata ttaccaatta
     5401 tgcaccattg ttcccgggtc agcgccagat gcaggtgcag cggagggttg acctggttcc
     5461 ggaaacggaa tgcattttaa aaaagaagat tacctaaatg gcaaaagcac aagcaaacag
     5521 cgcaaaagcc gctgctaaga aaaggatcgt aaaagtagat gtctatggcg atgcgcatat
     5581 caccgccagc ttcaacaaca tcatcatcag cctcaccaac aagaacgggc aggtaatttc
     5641 ctggagcagt gccgggaaga tgggtttcaa ggggtcgaag aagaatactc cctatgccgc
     5701 ccagatggcc tcttcggatg ccgccaagac ggccgtggat gcgggcctga agaaggtgga
     5761 tgtgtatgta aagggccccg gttcgggccg ggagagcgct atccgcgccc tttccaacaa
     5821 tggcatcgaa gtggtgatga tcaaggatgt gactccgctg ccgcacaacg gttgccgtcc
     5881 ccccaagaaa cgcagggtat agtaaagcga tcgcttcgcc gatagggtgg ggcggtcagt
     5941 cttatttata aagccgggta agattcaatt ttaagatttt tgaataatct tgcccgggta
     6001 actcaaaaat ctaacaaagc acattatggc acgttacaca ggaccgaaga ccagaatttc
     6061 ccgaattttt ggcgagccta tcttaggcaa tggcaagtat ctgacaaaga acagcaaccc
     6121 tcccggccag cacggcggga ccaagaagcg caaggctccc ggtgagtatg cgcttcagct
     6181 gaaagaaaag caaaaggcca aatacaccta cggggtgctc gagcgccagt tccgcaagac
     6241 ctttgacgaa gccaacaggc gcaagggggt aaccggtgag aacctcatca agctgctgga
     6301 atcccggctg gacaatacgg tgtatcgcct cggcgta
//
LOCUS       DATGFG010000484         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_793717, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000484 DATGFG010000000
VERSION     DATGFG010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793717"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VKQ52_08990"
     CDS             <1..310
                     /locus_tag="VKQ52_08990"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HLZ87364.1"
                     /translation="SLYPSDTNWASYNASCAANMNDMVARYNKPVMISEVGMPANEAA
                     TCEAFITDLIHKVKAVSGGKGLGVFYWEPECYNNWQGYGLGAFDNTGKPTVAMNAFAQ
                     "
     gene            324..>2408
                     /gene="galA"
                     /locus_tag="VKQ52_08995"
     CDS             324..>2408
                     /gene="galA"
                     /locus_tag="VKQ52_08995"
                     /inference="COORDINATES: protein motif:HMM:NF041462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HLZ87365.1"
                     /translation="MKSLLLSIVFLPLLALAQSTPRQSTTVSTGLRQSTGGLPRLHQP
                     FDAGWKFHLGDAADAAKDFNYRTASVFVKTGKAAGTAIAPDFDDRNWRTLSLPHDWAV
                     ELPFENSPDSLVMSHGYKPVGGHYPATSIGWYRKHFTVAAADSGGRFIIHFDGIYRDS
                     KVWINGFYCCGNASGYSGFSCDITDYLRYDHDNVLVVRVDASQYEGWYYEGAGIYRHV
                     WLNRYNNLHIAEDGLFVHCAGSTTAAVTVETTIANDNPSPATTTVSAFLTDREGKLLG
                     RAPAEPLTLNPNEHRTVIQTISIQHPIRWSPESPYLYRAGATLRRGNTIIDEEKLRIG
                     IRTIRIDAGGVFVNGRYTKLKGVNNHQDHAGVGAAMPDYLQYYRARLLKQMGVNASRT
                     SHNPPTPEWLDACDSLGLLVVDENRLLNSGAEYLGQLHRLILRDRSRASVFLWSLGNE
                     EQMVQTRTVGRRIAATLIQHQREWDPTRISTYGADLANVYQGINEVIPVRGFNYRILG
                     VEPYHKDHPDQPIIGTEMGSTVTTRGIYVKDTVNAYLPDEDIYAPWWANTAEQWWALA
                     ADKPYWLGGFIWTGFDYRGEPTPYSWPNISSHFGVMDMCGFPKNIYYYYKSWWTDEDV
                     LHISPHWNWKGKEGQPIPVWVNTNAEEVELFLNKKSLGKKAMPRNSHLEWSVPYEPGR
                     LEAVATKKGKKLT"
BASE COUNT          541 a          831 c          594 g          442 t
ORIGIN      
        1 gtccctctac ccttcggata ccaactgggc ctcctacaac gcctcctgcg cggcgaatat
       61 gaacgatatg gtcgcccgct ataataagcc ggtgatgata tccgaagtag gcatgcctgc
      121 caacgaggcc gccacctgcg aagcctttat caccgatctt atccataagg taaaggccgt
      181 ctccggcgga aagggcctcg gcgtcttcta ctgggaaccc gaatgctaca acaactggca
      241 ggggtatgga ctcggggcct tcgacaatac cggcaaacct accgtcgcca tgaatgcatt
      301 tgctcaataa aaaatcgact cttttgaaat cgctactcct atcgatcgtc ttccttccct
      361 tgcttgccct ggcgcaatcg actccgcggc agtccaccac ggtttccacc gggctgcggc
      421 aatccaccgg gggcctcccg cgcctccacc aacccttcga cgccggctgg aaattccatc
      481 tcggcgacgc cgccgatgcc gcaaaggact tcaactatcg caccgcctcc gtctttgtaa
      541 aaaccggtaa ggccgccggc actgctatcg cccccgattt cgatgaccgc aactggcgca
      601 ccctctctct tccccacgac tgggcggtcg aactgccctt cgagaattcc cccgactccc
      661 tggtcatgtc ccatggctat aaaccggtcg gcggccacta tcccgcgaca agcatcggct
      721 ggtaccgcaa acatttcacc gtcgctgccg cggattccgg cggccgcttc atcatccact
      781 tcgacggtat ctatcgcgac agcaaggtct ggataaacgg cttctattgc tgcggcaacg
      841 ccagcggcta tagcggcttc tcctgcgaca tcaccgatta tctgcggtat gatcacgaca
      901 atgtcctcgt cgtccgcgtc gatgcctccc aatacgaagg ctggtactac gaaggcgccg
      961 gcatctaccg tcatgtatgg ctgaaccgat acaataacct gcatattgcc gaagacggcc
     1021 tgttcgtaca ttgtgccggt agcacaacgg ccgccgtcac ggtcgaaacg accatcgcca
     1081 acgacaaccc cagccccgcc accaccaccg tctccgcctt cctcaccgac cgcgaaggca
     1141 aactcctcgg ccgcgcgccc gcggagcccc tgaccctaaa cccaaacgaa caccgcaccg
     1201 tcatccaaac gatttccatc cagcatccca tccgctggtc acccgaatcg ccctacctct
     1261 atcgcgccgg ggccaccctc cgcaggggca acaccatcat cgatgaggag aagctgcgca
     1321 tcggcatccg caccatccgg atcgacgccg gcggtgtctt tgtcaacgga cgctatacaa
     1381 agctcaaggg tgtcaacaac caccaggacc acgcaggtgt cggcgccgcc atgcccgact
     1441 atctgcaata ttaccgtgcc cggctgctaa aacagatggg tgtcaacgcc agccgcacga
     1501 gtcataatcc tcccacaccc gaatggctcg acgcctgtga cagcctcgga ctgctggtcg
     1561 tcgatgaaaa tcgcctgctc aacagcggcg ccgaatatct cggccagctc caccgcctga
     1621 tccttcgcga ccggagccgg gcctccgtat tcctctggag cctgggcaac gaagaacaga
     1681 tggtgcaaac ccgcaccgtc ggccggcgca tcgccgccac gctcatccag caccagcggg
     1741 aatgggatcc cacccggatc agcacctatg gcgccgatct cgccaatgtc taccagggca
     1801 taaacgaggt catccccgtt cgcggcttca actatcgcat cctcggcgtg gaaccctatc
     1861 acaaggacca tcccgaccag cccatcatcg gcaccgagat gggcagcacc gtcaccaccc
     1921 gcggcatcta tgtaaaagat accgtcaacg cctacctgcc cgatgaagat atctatgcgc
     1981 cctggtgggc caacaccgcg gagcaatggt gggccctggc ggcggacaaa ccgtattggc
     2041 tgggaggatt tatctggacc ggcttcgact accgcggcga acccaccccc tatagctggc
     2101 ccaatatcag ctcccacttc ggggtcatgg acatgtgcgg cttccccaag aatatctatt
     2161 actattataa atcctggtgg acggacgaag acgttcttca tatctctccc cactggaatt
     2221 ggaagggtaa ggaaggacag cccatccccg tttgggtcaa caccaacgcc gaggaggtgg
     2281 aactctttct caataagaaa agccttggaa aaaaagcgat gccccgcaac agccatctgg
     2341 agtggagcgt accctacgaa cccggtcgcc tcgaggccgt ggcgacaaaa aaaggaaaaa
     2401 agctgacg
//
LOCUS       DATGFG010000485         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_347057, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000485 DATGFG010000000
VERSION     DATGFG010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347057"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..660)
                     /locus_tag="VKQ52_09000"
     CDS             complement(55..660)
                     /locus_tag="VKQ52_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ87366.1"
                     /translation="MAETLQRDEIIVKEIVDTARALFKKSGFKKTTMGDIARSLGKAK
                     SSLYYYYPSKEDIFEAVIYAEMDELLDEIHHALDAATNSKEKLKAYCRCRLEKLNELC
                     NLSDALKSEIAELHCVMAEMKSTFDTTHVELVKSILVEGVSNGEFKKINEDNIELIAY
                     LMVSSFRGLAVPLMVGQARSPRLDLQIDSIVDIMVEGIGRR"
     gene            804..1205
                     /locus_tag="VKQ52_09005"
     CDS             804..1205
                     /locus_tag="VKQ52_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ87367.1"
                     /translation="MSIQLNCILLIDDDEPTNFLNKMTLEQAGCTRQIRIAQSGQEAL
                     DYLREATTRPDLIFLDINMPAMDGWEFLERYRNLPTDRKADIVLIMLTTSLNPDDEKR
                     TRAIPEVAGFENKPLNQEQLDNLLKKYFSKS"
     gene            complement(1245..1679)
                     /locus_tag="VKQ52_09010"
     CDS             complement(1245..1679)
                     /locus_tag="VKQ52_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLZ87368.1"
                     /translation="MKKVADILSHKGARITSVSPQTTVLDALRIMADQNIGSVLVVEG
                     NQYLGIMTERDYSRKVILKGKSSTDTPVAEIMSKDFPGVTPNDTVEFCMELMSDKNIR
                     YLPVFENEQLCGIVSINDLVRETIRTHEETITHLKDYLHSGI"
     gene            1872..>2242
                     /locus_tag="VKQ52_09015"
     CDS             1872..>2242
                     /locus_tag="VKQ52_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HLZ87369.1"
                     /translation="MRTAFPIILLALNLLPPILPIQTVSAQSPAASAQQAIPPPAVVL
                     NQLGFYPEAPKFAVVTGLVIKDVFYIVSVADNAPSWQGRVDTVFFGRLGPLTSSANSS
                     VTTRLADFSAVHRLGVYRVVVP"
BASE COUNT          504 a          726 c          455 g          557 t
ORIGIN      
        1 tcctgccccg gctcaatagc tcaactcctc ctgcctgcgc caggtaggcg ctgtctaacg
       61 tctgccaatc ccttcaacca taatatctac gatcgaatcg atctgcagat ccagccgagg
      121 gctccgggcc tgccccacca tcagcggcac cgccagcccc cgaaaactac tcaccatcag
      181 gtaggcgatc agctcgatat tatcttcgtt tatcttcttg aattctccat tgctgactcc
      241 ttccaccagg atactcttta ccagctccac atgcgtcgtg tcgaacgtac tcttcatttc
      301 ggccataaca cagtgcaatt cagcaatttc actcttcagt gcatcgctta ggttacaaag
      361 ctcgtttagt ttctccagcc ggcaccggca ataggccttc agcttctctt tcgaattcgt
      421 ggccgcgtcg agggcatgat ggatctcatc cagcaattcg tccatttcgg catagatcac
      481 ggcctcgaaa atgtcctcct tgctcggata atagtagtag agagagctct tcgccttgcc
      541 aaggctccgg gcaatatcgc ccatcgtcgt cttcttgaac cccgatttct taaaaagggc
      601 tctggcagta tcgactatct cctttactat gatctcatcc cgttgaagcg tttcagccat
      661 aagggcaaat ttacacgttt tcctccccga atctggttaa tagctcgtga atcttgcatc
      721 tctgcgggac aatatataca tttgtcccac ccttcaccct aggtaagaca acctcccatt
      781 cttccattca acccctgtaa aacatgagta ttcaacttaa ctgtattctt ctcatcgatg
      841 acgatgaacc gacaaatttt ctgaacaaga tgaccctgga acaggccggc tgcacccgcc
      901 agatccgcat cgcgcaaagc ggacaggagg ctcttgacta tctgcgggaa gccaccaccc
      961 gtcccgacct catcttcctc gacatcaaca tgccggccat ggatgggtgg gagttcctcg
     1021 aacggtatcg caatctcccc accgaccgca aagccgacat cgttctgatc atgcttacta
     1081 cctcgctgaa ccccgacgac gaaaaaagaa cccgggccat ccccgaagtt gccggcttcg
     1141 agaacaaacc gctcaatcag gaacagctcg ataacttgct gaaaaaatac tttagtaaaa
     1201 gctaaccgcg ccgcaccccc acaccggccc gcgaacttca cgccctagat ccccgaatgc
     1261 aaatagtcct tcagatgggt gatcgtctcc tcgtgtgtcc ggatcgtctc cctgaccaga
     1321 tcgttgatgg aaacgatgcc gcacaactgc tcgttctcga acaccggcaa ataacggatg
     1381 ttcttgtcgg acattagctc catacagaac tccaccgtgt cattcggcgt cacaccgggg
     1441 aagtctttcg acatgatctc ggcaacgggg gtgtcggtgg aagacttacc cttcaggata
     1501 accttccgag aataatcccg ctccgtcatg atcccaaggt attggttccc ctccaccacc
     1561 aggaccgatc cgatgttctg gtcggccatg atccgcaggg cgtccagcac ggtcgtttgg
     1621 ggactcacag atgtgatacg cgcccctttg tgagaaagaa tatcagctac ttttttcata
     1681 cagcctgatt tttgggtgag gaaacatttt ttagttcgta aaagccgcca caggcggcta
     1741 ttgcatattt tcaatttacg aaatattaac aaaagcccac ctctcccgga cacttttatt
     1801 cttttcggtc cgcatccccg gcgcattcat atccctcgca tatttatctt tgccaacatc
     1861 aattacatcc catgcgcact gccttcccga ttatcctcct ggccctcaac cttctgcctc
     1921 ccatacttcc tatccaaacg gtatccgctc aaagcccagc cgcatccgcc caacaggcta
     1981 tcccaccccc cgccgtcgtc ctcaaccaac tcggtttcta tcccgaagcc ccgaaattcg
     2041 ccgtcgtcac cggcctcgtc ataaaagatg tattttacat tgtttctgtt gccgataacg
     2101 caccttcctg gcagggccgc gtcgacaccg tcttcttcgg ccgcctcggc ccgctgacct
     2161 cctccgccaa ttcctcggta accacccgtc tcgcggactt cagtgctgtt catcgcctgg
     2221 gcgtctaccg cgtcgttgta cc
//
LOCUS       DATGFG010000486         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_469043, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000486 DATGFG010000000
VERSION     DATGFG010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469043"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..1251
                     /locus_tag="VKQ52_09020"
     CDS             394..1251
                     /locus_tag="VKQ52_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HLZ87370.1"
                     /translation="MRQLKIATQITNRDSQAVEKYLQEISKIPMITPEEETVLAQRIK
                     MGDQKALDKLVQANLRFVVSVAKQYQHQGLSLSDLINEGNLGLIKAAQRFDETKGFKF
                     ISYAVWWIRQSILQALAEQGRLVRLPQNKIGTYNKANKAYMAFEQEHEREPSTEELAE
                     LLEMSETEINNIFQSNTRHSSLDAPVHEAEDVAMGDLLEGGDETDDDVMKDSLRAEIR
                     RVLKSLSPREAEIVNAYFGLDGENGVTIEQIGQKYDLTKERIRQIKERAIKRLQKARY
                     SNALKAYLG"
     gene            1506..2453
                     /gene="trxB"
                     /locus_tag="VKQ52_09025"
     CDS             1506..2453
                     /gene="trxB"
                     /locus_tag="VKQ52_09025"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HLZ87371.1"
                     /translation="MEQTPSEKVHCLIIGSGPAGYTAAVYAARANLRPVLYQGIQPGG
                     QLTITTEVENYPGYPDGVQGPEMMVHFEKQAMRMGADIRYGLATKVDFSGHPHRVWID
                     EEKLIEADSVIIATGASAKWLGLESEQRLNGYGVSACAVCDGFFFRNKEVAIVGAGDT
                     AAEEALYLSKLCTTVHMLVRKDRMRASRVMQERVQQTPNIRMYWNSEADEVLGESKVD
                     GLRIRNNKTGEKKDILVSGFFVAIGHQPNSAIFSDFIEMDEAGYIRTVPGTSKTNVEG
                     VFAAGDVQDKIYRQAVTAAGSGCMAALDAERYLSAKGLA"
     gene            2530..2889
                     /locus_tag="VKQ52_09030"
     CDS             2530..2889
                     /locus_tag="VKQ52_09030"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HLZ87372.1"
                     /translation="MVTVQLHNLIFSGRHGVFKEELATGNTFEVSLDVSYDESGKPFS
                     GLDSIVNYVTLFAIIKERMHQPSPLLEKIAEEIIVKIREQYPFIREAILSIYKLQAPI
                     GNFQGKAGMTLRKEFGE"
     gene            complement(2929..>3542)
                     /locus_tag="VKQ52_09035"
     CDS             complement(2929..>3542)
                     /locus_tag="VKQ52_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLZ87373.1"
                     /translation="SPLVFQYVVSIVAWVFFYILIEHHGRQALAISNAMRNIFGLFGV
                     FTWAFASTTNAMVSNIIGQKMEDKVITLIGKIMKLSLSVSILIFILLNCFPGLLLSIY
                     GQDEAFVRAAIPVLRIVSSAVVLMSFSIVWLSAVTGTGNTRINLLIEVITIVVYSLYV
                     WFTLEKWNMSIEVGWLSEWVYWLCIFSMSFAYIRSGRWKGKVI"
BASE COUNT          888 a          792 c         1084 g          778 t
ORIGIN      
        1 catcgaggct aaaatcgtcg gccgggtgga agcggcagcc gcgaaaagcc tggtaataag
       61 gaccggagga gaagaaatcg tttattaaat gttaaaagcg gctccaggct accggcgagc
      121 cttatctgca aagggtacgg gaagattgaa aggcgacaaa tttgctgctt gctttctcct
      181 actttggtaa aaaaagttta aaaagctctc aatttattcc acagggctat gaattttcat
      241 ttttgatgag tactttcgca aacggttctg gacggataga cattaattta tttttatatc
      301 ttttttgccc ctccgtgaac tctcccggct tgatttgtgt ttattattag taccctacag
      361 agattttgtc atcttttatt attattatcc gatatgaggc aacttaagat tgctacacag
      421 attacgaacc gtgattccca ggcggttgag aagtatttgc aggagatcag caagatcccg
      481 atgatcacgc ccgaggagga aactgtcctc gctcagcgga tcaagatggg cgaccagaag
      541 gcgttggata agttggtgca ggccaacctt cgctttgtgg tgtctgtggc aaaacagtat
      601 caacaccagg ggttatcact gagtgatttg atcaacgagg gcaacctcgg ccttattaaa
      661 gcggctcaac gctttgatga aaccaagggt ttcaagttta tttcgtacgc ggtatggtgg
      721 atccgccagt ccattttaca ggcgttggca gagcagggca gattagtccg cctgcctcaa
      781 aacaaaattg gcacctataa caaggctaac aaggcctata tggccttcga acaggagcat
      841 gagcgcgagc ccagcaccga agaattagcc gagttactcg agatgagtga gacggagatc
      901 aacaacattt tccagagtaa tacccgtcac agcagcctgg atgccccggt tcacgaggca
      961 gaggacgtag ccatgggtga tctgctggaa ggtggtgatg aaacggacga tgatgtcatg
     1021 aaagattcct tacgcgcaga gatccgccgt gtgctcaagt cattgagccc ccgtgaagcg
     1081 gagatcgtca atgcttattt tggcctggat ggcgagaatg gggtgaccat cgagcagatc
     1141 gggcagaaat atgatctgac gaaggagcgt atccgccaga tcaaggagcg ggcgatcaaa
     1201 cgcctccaaa aggcgcgcta cagcaatgcg cttaaggcat accttggata atttcctgta
     1261 aatcactcaa aatcaataca atcccgatca tttgatcggg atttttattt gcctgggagg
     1321 cccttggggc cgggaaaagc cggctattcg ggcgatcggt tgcatgttgg gagcagtttg
     1381 aataaaatcg gttgaaaaat agtgaaatat tgagtcattc aaccgacatt ccgctatgga
     1441 atttcgcatt agatttgccc gccaaaaatt tgaattatat ttatccgcca aaaaataaac
     1501 gcagaatgga gcaaacccca agcgaaaaag tacattgtct gatcatcggc tcggggccgg
     1561 ccgggtatac ggctgccgtc tatgccgccc gcgcgaacct ccggcctgtc ctgtaccagg
     1621 gaattcagcc gggtggacag ctgacgatca ccacggaagt agagaattac ccgggctatc
     1681 ctgacggggt gcaggggccc gagatgatgg tgcattttga gaaacaggcg atgcggatgg
     1741 gcgccgatat ccggtatggc ctggccacga aggtggattt ttccgggcat ccgcacaggg
     1801 tctggatcga cgaggagaag ctgatcgaag ccgattccgt cattatcgcc accggtgctt
     1861 cggccaaatg gctggggctg gagagcgagc agcggttgaa tggatatggg gtgagtgcct
     1921 gcgcggtttg tgacgggttc tttttccgga acaaggaagt cgcgatcgtc ggcgccggag
     1981 atacggctgc cgaggaggcc ttgtacctgt cgaagctttg tacgacggtg catatgctgg
     2041 tgcgcaaaga caggatgcgg gcatcgcggg tgatgcagga gcgggtgcag cagacgccga
     2101 acatccggat gtactggaac agcgaggccg atgaggtgct gggggagagc aaggtggacg
     2161 ggctgcggat caggaacaac aagaccgggg agaagaagga tatcctggtg agcgggttct
     2221 ttgtggcgat cggtcaccag cctaattcgg ccatcttttc ggattttatc gagatggatg
     2281 aggcggggta tatccggacc gttcccggga cgagcaagac caatgtagaa ggggtttttg
     2341 cggcggggga tgtgcaggac aagatctacc ggcaggccgt aacggctgcg ggcagcggct
     2401 gtatggcggc gctggatgcg gaacgctatc tttcggcaaa gggactcgca taggccgggg
     2461 tcgcgaagaa tagcattgac ggtgtcacga agaatagcat gggtggggct gcggtaaaaa
     2521 aacaaacgaa tggtcactgt acaactacat aacctgatct tctccggcag gcatggggtc
     2581 ttcaaggagg agctggctac gggcaatacg ttcgaagtga gccttgatgt gtcctatgac
     2641 gagagcggga agcccttcag cgggctggat tcgatagtca attacgtcac gttgttcgct
     2701 atcatcaagg agcggatgca tcagccttcg ccgttgctgg agaagatcgc ggaagagatc
     2761 atcgtcaaga tccgggagca gtatcctttt atccgggaag ccattctttc tatctataag
     2821 ttacaggcgc cgatcggcaa tttccagggc aaggcaggga tgacgctgcg gaaagaattt
     2881 ggcgagtgag ggttgccgtt gatagtgggg cctgtgcagc cgctgatctc agatgacctt
     2941 gcctttccag cggccgctgc ggatataggc gaaggacatg ctgaagatgc acaaccagta
     3001 tacccattcc gagagccagc ccacttcgat ggacatgttc catttttcca gtgtaaacca
     3061 tacatagagg ctatagacga cgatcgtgat cacctcgatc aggaggttga tgcgggtatt
     3121 gcccgtgccg gtgacggcgc ttagccagac gatggagaag gacatcagga cgacggcgga
     3181 ggagacgatc cggaggacgg ggatggcagc ccggacaaag gcttcgtcct ggccgtagat
     3241 ggacagcagc agaccgggga agcagttgag caggatgaag atgaggatcg acacggagag
     3301 gctcagcttc atgatcttcc cgatgagggt gatgaccttg tcttccattt tctggccgat
     3361 gatattgctt accatggcat tggtggtgga ggcaaaggcc caggtgaaga cgccgaagag
     3421 accgaagata ttgcgcatgg cgttgctgat ggcgagggcc tggcggccat ggtgttcgat
     3481 caggatatag aagaagaccc aggccacgat gctgacgacg tactggaaga cgagggggga
     3541 gg
//
LOCUS       DATGFG010000487         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_117262, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000487 DATGFG010000000
VERSION     DATGFG010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117262"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VKQ52_09040"
     CDS             <1..645
                     /locus_tag="VKQ52_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HLZ87374.1"
                     /translation="YANSAFNATGNGERLIVSNNGAYKVDTAFSVSCDFMIRSNAYYS
                     GGYDFSGLQVFLSIVNVTTGTGPTFDLGLTRPEAPQYFTFGVNGAQGADCSGYGNNNP
                     SNISDSTHFIPQLQSWYNVVCTFRKNTTSVYVNGVLVSTLTNPNTLSAVFCPDANFIV
                     GGWWNGGNSGDENIRGKLDEVRFYNRVLNAKEIAYLARNFQVTSNKASPGLKTR"
     gene            792..3035
                     /locus_tag="VKQ52_09045"
     CDS             792..3035
                     /locus_tag="VKQ52_09045"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HLZ87375.1"
                     /translation="MGRLIIISNRLPFSLDKDGDKVSLRQSSGGLVSAIKSYFENGAA
                     QRGDITEKMWMGVADFPQEDWDQAVAAHAASQDFGILPVFIDKDLYDNYYNGFSNSVL
                     WPLFHYFNNLAAFESHYFDAYVQVNQLFAEKLIPLLQPGDQVWVHDYQLMILPHLLRS
                     KRPEATIGFFLHIPFPSYEIFRLMPTEWKKILLQGVMGADLIGFHTYDYVQHFLQSVK
                     MLLGVDSYFHNLQYQDRLVRIDLFPIGIDYNKFHQGTTDPEVEELRNKVLDNFTDKKI
                     VFSVDRLDYTKGLMQRLSGFEYFLERYPEWREKMVFILNVVPSRDTIPAYNDRKKSIE
                     EKIGMVNGRFSTISWQPVIYRYNHLPFKELIALYQAADVALITPLRDGMNLVAKEYVA
                     ASLGRGVLILSELAGAASELSEAILVNPTDTADVASAITRALAMPLYEQRNRISLMQR
                     RLQEYDVFKWVNDFMDQLAQVKQEQQKQKVKLLDEKAIAAIRKHYQKSRNRCLMLDYD
                     GTLVPFSRIPSEASPDNAVKELLSRLSSDPRNHVAIISGRDLSSLDRWLGEFNITLVA
                     EHGASFKMRGGSWQQMVSVSDMWKEEIRRIMQLFVLRCAGSFIEEKTNTIAWHYRNTQ
                     SGLGFSRSRELLNTLSQLIQNTTLQVIDGNKVVEVRIAGFDKGAMALKIVGEMDPDFV
                     LCMGDDTTDEDMFKALEGEAYTIKVNGGASAAQYTLLSQQKVLPILNTLTSDSQVSLP
                     EKKYADS"
BASE COUNT          741 a          854 c          804 g          694 t
ORIGIN      
        1 tatgccaaca gcgccttcaa cgccaccggc aatggggaaa gactgatcgt ttcaaacaac
       61 ggtgcttaca aagtggacac tgccttttcc gtttcctgcg attttatgat ccgaagcaat
      121 gcttattatt ccggaggcta tgatttcagt ggcttgcagg tcttcctgag cattgtcaat
      181 gtcaccaccg gcaccggccc cacgttcgac cttggtttga cccggcccga agcgccgcag
      241 tattttacct ttggcgtcaa cggcgcgcaa ggcgccgatt gcagcggcta cggcaataat
      301 aacccgtcaa acatcagtga ttccacccat tttattccgc aactgcaaag ctggtacaat
      361 gtggtttgta cttttagaaa aaacaccact tctgtctacg tcaacggagt attggtaagc
      421 actctcacga acccgaatac actttccgcg gtgttctgtc cggatgccaa tttcatagtc
      481 gggggctggt ggaacggtgg caacagcggc gacgagaata tccggggtaa attagatgag
      541 gtccggttct ataaccgggt gttgaacgcg aaagagatcg cctatctggc caggaacttc
      601 caggtgacgt ccaacaaggc gagccccgga ctaaagaccc ggtgatctga tcatcgaaga
      661 atagcattca ttgaccgaaa tttcttgtaa gagcttcccc ccggggagct cttttatttt
      721 atggacttat tcttggcccc attgttgaat acaggtcaaa atggagtatc cctattaaaa
      781 ggattacaat aatgggtcgt ttaatcatta tatccaaccg gctacccttc tccctggata
      841 aggatgggga taaggtaagc ctgcgacaaa gctccggtgg attggtctcc gcgataaaga
      901 gctatttcga gaacggggcg gcacagcggg gggatataac ggagaaaatg tggatgggtg
      961 tagcggattt tccccaggag gactgggacc aggccgtcgc cgcccatgcc gccagccagg
     1021 attttgggat actgcccgtt ttcattgata aagacctgta tgacaattac tacaacggct
     1081 tttccaactc ggttctctgg cctttgttcc actattttaa caatctcgcc gccttcgaaa
     1141 gccactattt cgacgcctat gtacaggtga accagctttt cgcggagaag ctcatcccgc
     1201 tgctccagcc gggtgaccag gtctgggtac acgattatca attgatgatc ctgccgcatt
     1261 tactgcggtc aaaaagaccc gaagcaacca tcggtttttt cctgcatatc ccattcccct
     1321 cctatgagat cttccggctc atgcccaccg agtggaaaaa aatactcctg cagggggtga
     1381 tgggcgccga tctgatcggg tttcacacct atgactatgt ccagcacttc ctccagtcgg
     1441 tgaagatgct cctgggcgtc gatagttatt ttcacaacct gcaataccag gacaggctgg
     1501 tgcgcatcga tcttttcccg atcggcatcg actataacaa attccaccag ggaaccacag
     1561 atccggaggt ggaagagctc cgcaataagg tactcgataa tttcaccgat aagaagatcg
     1621 tcttctccgt cgaccggctc gactatacaa agggcctgat gcagcggctc agcggcttcg
     1681 agtatttcct cgaacgctac cccgaatggc gcgagaagat ggtctttatc ctcaatgtcg
     1741 tgccttccag ggatacgata cccgcctaca atgacagaaa gaagagcatc gaagaaaaga
     1801 tcgggatggt gaacgggcgg ttctccacca ttagctggca accggtcatc taccggtata
     1861 accacctacc gttcaaggag ctcatcgcct tataccaggc ggcggacgtg gccctgatca
     1921 ccccgcttcg cgacgggatg aaccttgtcg caaaggaata tgtggccgcc agcctgggcc
     1981 gcggcgtgct gatcctgagt gaactggccg gcgcggcaag tgaactgagc gaggccattc
     2041 tcgtgaatcc caccgatacg gccgatgtgg cttccgccat cacccgcgcc ctggccatgc
     2101 cgctttacga acagcgtaac cgcatctccc tgatgcagcg ccgcttacag gaatatgacg
     2161 tcttcaaatg ggtaaatgat tttatggacc agttagcaca ggtgaagcag gagcagcaga
     2221 aacaaaaggt gaagctgctc gatgaaaagg ccattgccgc catccgcaaa cattatcaaa
     2281 agagcaggaa ccgctgcctg atgctcgact atgacggcac tctcgttccc ttctcgcgta
     2341 tcccctcgga agcatcgccg gacaatgcgg taaaggaact gctgtcccgg ctgtcctccg
     2401 accccaggaa ccatgtggcg atcatcagcg gccgcgacct gtcttccctc gaccgctggc
     2461 tcggcgaatt caacatcacg ctcgtggccg aacacggggc aagctttaaa atgcgcggcg
     2521 gctcctggca gcagatggtg tcggtatccg atatgtggaa ggaggagatc cgccggatca
     2581 tgcagctctt cgtccttcgc tgcgcggggt ccttcatcga ggaaaagacc aacaccatcg
     2641 cctggcatta ccggaatacc cagagcggcc tcggcttctc ccggagccgg gaattgctca
     2701 ataccctttc ccaactgatc cagaacacca ccctgcaggt gatcgatggc aataaggtgg
     2761 tcgaggtccg gatcgcgggt tttgacaagg gggccatggc gctgaagatc gtgggcgaga
     2821 tggatcccga cttcgtcctg tgcatggggg atgataccac ggatgaagac atgttcaagg
     2881 ccctcgaagg ggaggcctat accatcaagg taaatggcgg cgcttcggcg gcccagtata
     2941 ccctcctgtc gcaacagaag gtattaccca tcctgaatac actcacaagc gattcacagg
     3001 tgtcgctccc cgaaaaaaag tatgccgatt cataagtata acatgggcgt cgtcggtaat
     3061 tgctcttacc tcgcctatat cgatacccgg gcg
//
LOCUS       DATGFG010000488         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_44221, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000488 DATGFG010000000
VERSION     DATGFG010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44221"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VKQ52_09050"
     CDS             complement(<1..445)
                     /locus_tag="VKQ52_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87376.1"
                     /translation="MSYVSLENSESFLKCFEVIAEDKSCIVSDSVLYFSGKTGKGFIT
                     VAGVATGIEVLAVNVSMLTEDLTLRFLPSPESASYILGFDEIEIPGKGDNDLSVNQVG
                     AHVEKVHSAVSIADAVKGRTVTFPRDRIIRSLIVRVRKSSLDHLIK"
     gene            733..1248
                     /locus_tag="VKQ52_09055"
     CDS             733..1248
                     /locus_tag="VKQ52_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87377.1"
                     /translation="MNNSTKKDCVDTDLRSLLKHQHKRVYSICRLFTHNYKEHQHLFT
                     NIIAAASQNIRSRKDNSDDKQTMLLRACINMAALHSISLDLAPATDRTIQFKSPDYQR
                     SMSNFRATMGEISDLEKFRLFLEFEKVSPEEMTGLTGIPAGNRPVSRKKESEAARKNF
                     IPYLKEKLIWS"
     gene            1239..1868
                     /locus_tag="VKQ52_09060"
     CDS             1239..1868
                     /locus_tag="VKQ52_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87378.1"
                     /translation="MELKKLLQQWNSFPEISMEERPVLSSDLEKIVVKNPLSDAFYLK
                     RKLILRIAVFSILWLINGWQLRHQWITNGTELYPQGALFLLLAYSLYFHIRLLLFADY
                     PTLLALPLINFLNKLETILDKYILSFKSVSALAGFYLLFGVEWLLSRVSPGAAEGLSH
                     NDWYKWLILIFLSVSFDILTLHTIIPRYRKLLGTVKKYRDGIHTRAQNK"
     gene            1947..2576
                     /locus_tag="VKQ52_09065"
     CDS             1947..2576
                     /locus_tag="VKQ52_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87379.1"
                     /translation="MQNILIADDHSIVRAGLKLLINSCIRDVAIDEAEDGEAVIKKMK
                     SAHFDLLILDINMPHTESFSLTAYLLKEYPGLKIIIFTMNQEVFFAKRFLKIGAHGYL
                     NKYSKESEIKYAIQKVLGGKRYISDNLAEILSGELTKKLKDNPFEDLSDREFEVVLQM
                     LKGSSVSEIANTLSLHKSTVGTHKTRILDKLKISNTVELINLARLYEII"
     gene            complement(2593..3420)
                     /locus_tag="VKQ52_09070"
     CDS             complement(2593..3420)
                     /locus_tag="VKQ52_09070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HLZ87380.1"
                     /translation="MYKAVFLDMDGTLLRSDHSVSDETVQTIRALTDKGVSVILVSAR
                     PINAVLPTFRHIGLPAHTPLITLNGSYIVENGEPVFQAMIDLETTTRVTEEVRPFKAT
                     IAYYLQREWFSEVKDAWTDHEQKIMDVPIGIAPITRLVEGWSARSIGPNKMMVMSEPD
                     NIAVIQQHLRSLYNGRLNIYPSKATYLEVMDSRGSKSNGVRFVCERLGIDPSEIIAMG
                     DNYNDIEMIRFAGMGVAMGNAPDDIKAVADYVTDTNNNDGVRKAMEKFFGGVIPQGA"
     gene            complement(3455..>3784)
                     /locus_tag="VKQ52_09075"
     CDS             complement(3455..>3784)
                     /locus_tag="VKQ52_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87381.1"
                     /translation="VFMTVGLDLVLLSFLIYVIEFRQKTAWTSFFGVFGKNPLFIYLL
                     SELFIVILNIIPVRSDESFFEWVNKVFYQALFPGAFGSLLFALSYMLVCWSVGKILDK
                     KKIYIRV"
BASE COUNT          983 a          979 c          892 g          930 t
ORIGIN      
        1 ccttgatcag gtgatccagg ctggacttgc ggacccgcac gatcaggctg cggatgatcc
       61 ggtccctggg aaaggtgacc gtccggccct tcaccgcgtc ggcgatggac accgccgaat
      121 gcaccttttc gacgtgagcg cccacctggt tgacagacag gtcgttatcc cctttgccgg
      181 gaatttctat ttcgtcaaaa cctaagatat aagaagctga ttcgggagag ggcaggaagc
      241 ggagagtgag atcttcggtc agcatggaga cattgacggc caacacttcg atacctgtcg
      301 cgaccccggc gacggtgata aaacccttcc ctgtttttcc ggaaaaataa agaaccgaat
      361 ccgatacgat acaagacttg tcttctgcga tcacctcaaa acatttcaga aaggattctg
      421 aattttcaag agatacatag ctcattgcgt taattttatt cgcctgtcaa atggaacttc
      481 ttccggctac taatcagcga caaggtataa attagccctt ctcgttgtta tagtaaaaac
      541 atgaattgaa agtagttttt ttactataca tgtgtctgta aaaacactta gtaaattctt
      601 taccgggcct gccggatacc attccacggt ccttttacgt tattaaaatg catcgtacgg
      661 ttattcgaag tggaaatttt tgaaaatatt tactgccgga tccgtttgtt ttatgcataa
      721 aatccatacc caatgaacaa ttctacaaag aaggattgcg tcgataccga cctccgctcc
      781 ttattgaagc accagcacaa gagggtgtat tccatctgcc gtttgttcac ccacaattac
      841 aaggagcacc aacatctttt caccaacatc attgctgccg cctctcagaa cattcgttcc
      901 cgtaaagata acagcgacga caagcagacc atgctgttga gggcctgtat caatatggcg
      961 gcgctgcact ccatcagtct cgacctggcg cccgccaccg atcgcaccat acagtttaag
     1021 tcccctgact accagcgtag tatgagcaat ttccgggcaa cgatgggcga gatctccgac
     1081 ctcgagaagt tccggctctt cctggagttc gaaaaggtgt ccccggaaga aatgaccggt
     1141 ctcaccggta tccctgccgg taaccggcct gtctcgcgga aaaaggagag tgaagcggcg
     1201 cggaagaatt tcattcccta cctaaaggaa aaactcatat ggagctaaag aagttgctgc
     1261 agcaatggaa cagctttccc gagatctcca tggaagagag acccgtcctg tcctccgatc
     1321 tggaaaagat cgttgtaaag aatcccttgt ccgacgcttt ctatcttaag agaaaactta
     1381 tcctgcgtat cgctgttttt tcgatcctct ggctgatcaa cggctggcag ctgcgacacc
     1441 agtggatcac caacggaacc gaactgtatc cccagggagc gctgttcctt ttactggcct
     1501 attcccttta ttttcatatc cggctccttt tgtttgccga ctaccctacc ctgctggcgc
     1561 tacccctgat caatttcctg aataagctgg aaacgatcct ggacaaatac atcctgtcct
     1621 tcaaatctgt aagcgcgctg gccggctttt acctgctttt tggggtggaa tggttgctgt
     1681 cacgcgtcag ccccggcgcc gccgagggcc tgagccataa tgactggtat aaatggttga
     1741 tcctgatatt tctttcagta tccttcgata tcctgaccct ccataccatc atcccccggt
     1801 atcgtaaact gctgggtacg gtcaaaaaat accgggatgg catccatacc agggctcaaa
     1861 ataaataaaa agctgaaacg atattctgac ttcccaatat aactaatatc ctctatattt
     1921 gggttaagtt ccctcaataa acaaaaatgc agaacatctt aatcgcagat gaccattcca
     1981 tcgtcagggc agggttaaaa ttgctgatca atagctgtat cagggatgtt gctattgatg
     2041 aagccgaaga tggggaggcc gttatcaaaa aaatgaaatc cgcgcatttt gatctgctga
     2101 tcctggacat caacatgccc catacggaat ccttcagcct cacggcctat cttctgaagg
     2161 aatatccggg tttgaagatc attatcttca ccatgaacca ggaagttttt tttgctaaaa
     2221 ggtttttgaa gatcggggcg catggttatc tcaacaaata ctccaaagag tccgaaatca
     2281 aatacgccat ccagaaagtg ttgggtggaa aacggtatat cagcgacaat ttagccgaga
     2341 tcctctccgg cgaattgacc aagaaactaa aagacaaccc tttcgaagac ttgtccgatc
     2401 gggaattcga ggtggtgctg cagatgctga aaggcagttc ggtctccgag atcgccaata
     2461 cgctcagcct gcacaagtcg acggtaggta cgcacaagac gcgtatcctc gacaaattga
     2521 agatctccaa taccgttgaa ttgatcaacc tggcgagact ctacgaaatt atctaagcgc
     2581 cctgcgggat gactaagcgc cctgcgggat gacgccgccg aagaatttct ccattgcctt
     2641 tcttaccccg tcattattgt tggtatccgt gacgtaatcg gccacggcct ttatgtcgtc
     2701 cggcgcgttg cccatggcca cacccatccc ggcaaaccgg atcatttcga tatcattgta
     2761 attgtcgccc atggcgatga tctctgacgg atcgatcccg agccgttcac agacgaaccg
     2821 gacaccattc gatttggaac cgcggctgtc catcacttcg agataggtgg ccttggaagg
     2881 atagatattc agccggccgt tgtacagcga ccggaggtgt tgctggataa ccgcaatatt
     2941 gtcgggctca ctcatcacca tcatcttgtt gggaccgata ctgcgggcgg accagccttc
     3001 gaccagccgg gtgatggggg cgatcccgat cggcacatcc atgatctttt gctcgtggtc
     3061 ggtccacgca tcctttacct cggagaacca ctccctttgc aggtaatagg cgatagtggc
     3121 cttaaagggt ctcacctctt cggttacgcg ggtggtcgtt tccagatcga tcatggcctg
     3181 gaagaccggt tcgccgttct ccacgatata gctaccgttc agcgtgatca ggggcgtatg
     3241 tgcgggtaac ccgatatgcc ggaaggtggg cagaacggcg ttgatgggtc tggccgaaac
     3301 aaggatcacc gaaacgcctt tatccgtaag agccctgatg gtttgaaccg tttcgtcgct
     3361 gacggagtga tcactcctca gcagcgtccc atccatatcg aggaatacag ctttgtacat
     3421 tacattcatt tgggaccgca agatagggta caatctacac ccggatataa attttctttt
     3481 tatccagtat tttccccacc gaccagcaga ccagcatata cgacaaagcg aagagaagcg
     3541 agccgaaggc gccggggaac agggcctggt aaaagacctt gttcacccat tcgaaaaagc
     3601 tttcgtcgct tctcacggga atgatgttca ggattacgat aaacagctca gagagaagat
     3661 agatgaaaag cgggtttttg ccaaagacgc cgaaaaagga ggtccaggcc gttttctgcc
     3721 ggaattcgat cacatagatc aggaaggaca acagcaccag gtcgagaccc acggtcataa
     3781 aaac
//
LOCUS       DATGFG010000489         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1116545, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000489 DATGFG010000000
VERSION     DATGFG010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116545"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2393
                     /locus_tag="VKQ52_09080"
     CDS             <1..2393
                     /locus_tag="VKQ52_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ87382.1"
                     /translation="EADWQNAVFRTGLRQNYNLSIRGGSDKVQTAFSAGYFDQKGIVL
                     GAFFTRYNLSANVDYTPFSWLKSQTSMKFTRGETALPFPTGGQGASAGIGYLTKLPPT
                     LDGGNLLTPLIKDGRGNYGYYNPNNQAVRNWGDGPVWAIETQDQKNLTNYFLGTTSLE
                     ATILPGLTLKTNVGVNTNGYTGYYFTPSDTRQLDQYGTGTTTTNNFYSQSSNTTFEWL
                     WENTVAYKKTFGDHNIDFVGGYSAQENTFSQMGGQGNNLVSNGLRDLEDLPALTSVYG
                     NQVTTTLVSEFARLNYAYKDKYLLTGTIRRDGTSKFAPDHQYGVFPSGSVAWRIKEEN
                     FLQNVNWLSDLKIRGSYGQTGNQLNVQPFVYLAQYTSGPGATNQGNNGYPFNKTYQPG
                     LILTQQPNSNLKWEVAKMTDIAVDAGFLDNKLTVTADYYHKVSSNFLLYLPIPGQSGF
                     TYQYQNVGSILNEGVELSVNWAHTAGDLHYNIGVNLTTIKNRLLSLNSHLNEVPNLVN
                     LGFSTTGTNNWGTYSMTKVGGPVGEFYGYKTAGIFQTQKEIDDLNAAAAAKYGAGTMY
                     EPTSGPGGSAVPGDRKFVDLNHDGRITPDSDQVALGSPIPKIFGGITFDGSWKNWDFS
                     VFFYGTAGNKIFNYQERTLESFETSTGSVGLENIGLKYYQNAWTPTHPSNRYARIDAN
                     EFNTNTRPSDVYIEDGSYLRLKNLTIGYTLPLKTSGAFAPRIRVYITAQNLFTITKYT
                     GADPEIGQPQGTDVQDMSVIGTSARGSSVRSTTASGIDVGTYPNSRFYILGFNVTF"
     gene            2419..4053
                     /locus_tag="VKQ52_09085"
     CDS             2419..4053
                     /locus_tag="VKQ52_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87383.1"
                     /translation="MKNKRNIIWVAVLVLLVPLACSRNFLNPSDTQSANGSSLFHTPQ
                     DAISLVNAIYDGFQHDNQNFVLKALWYNANYTTQDFFNWGADVSYNTYQFPTDFASLP
                     VYWNDSYQGIARANSAFPVIAQMRTQGVLTDSLANFLLGQAYFLRGVYYYYLACTFGG
                     VPLELNVTTDGFHPRSTQDSVFMRVVKDMDSASLLLPWPQQLAAGDLGRATKGAALGY
                     KGAAEMWLKDYKDAITDFQALIPHYTLLPNFMSIHEYSNQNNAESLFELQFHLPSSST
                     VPDWSYNNNEVTWQSSFMWPWETSNFGYTYANKKLYTSFEAGDLRKPATIIGPDDSIQ
                     SPGIVAIGGIRNYSFVIQGFQGKLSLPASHFTGTDGKIMNTCGKTGDLWTGDQPGQPR
                     SGYYPMKYWRDPNVSGNSPSQIDPQHTNHIFGDQNITLLRLGEIYLDLAECFYKTGDQ
                     TDATNALMTVRNRAWGGSAPASPFGPDFMQIMLQEYRHELGGEMSTWYNMRRTGLQIQ
                     YIQNTFGITIPPGHDLLPIPQSAIATNPFLKQNPGY"
     gene            4054..>5340
                     /locus_tag="VKQ52_09090"
     CDS             4054..>5340
                     /locus_tag="VKQ52_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ87384.1"
                     /translation="MKGHYLSFLLAASIFCSCRNGTLFERVPSSHSGITFSNTIVETD
                     TINPLDVVNIYNGGGVGIGDFNNDGLPDIYFGGNRVPSRLYLNKGNFTFEDITEKAGV
                     AGLGRWARGISVVDINNDGLPDIYICNTIYRDSLLRRNILYVNQGIDKDGIPHFRDMA
                     AEYGLDAHYQSTMASFFDYDNDGDLDVYITVNEAYNGDAPTTFLRRSDRTAAPSRGRL
                     FRNDWDSTLGHPVFHDVSDQAGISFEGYGHAATVCDINNDGWKDIYVSDDFLSNNILY
                     INNHNGTFTNHTRDYLKHTSYNSMGQDVVDINNDGLPDIVELDMNPEDNYRKKMMLAS
                     ESYLTYQNFDQFGYEFQYVRNTLQLNRGRQLGEGDSLGIPAFSEIGYMSGISQTDWSW
                     SPLVADFDNDGYRDIIVTNGFPRDVSDHDFMAYREEH"
BASE COUNT         1275 a         1672 c         1347 g         1046 t
ORIGIN      
        1 atgaggccga ctggcagaat gccgttttcc gcaccggcct gcgccaaaac tataacctct
       61 ccatccgcgg cggctccgac aaggtgcaga ctgctttctc cgccggctac ttcgatcaga
      121 aggggatcgt actgggcgcc tttttcaccc ggtataacct cagcgccaat gtcgattata
      181 ctcccttctc ctggctcaaa tcgcagacca gcatgaaatt tacccgcggg gagaccgcgc
      241 tgccctttcc caccggcggc cagggcgcaa gcgcgggcat cggctacctg accaaactgc
      301 cccctaccct cgatggcggt aacctgctga ccccgctgat caaagacgga agaggcaatt
      361 atggttatta taacccgaac aaccaggccg tgcgcaactg gggcgacggt ccggtctggg
      421 ccatcgagac gcaggaccag aagaacctta ccaactactt cctcggaacc acttccctcg
      481 aagccaccat cctcccgggc ctcaccctaa agacaaatgt gggtgtcaac accaacggct
      541 acaccggcta ttattttacg ccttccgata cccgccagct ggatcaatat ggtaccggaa
      601 cgacaacgac aaacaatttc tatagccagt cctccaacac caccttcgaa tggctctggg
      661 aaaatactgt ggcgtacaaa aagaccttcg gcgatcataa catcgatttc gtaggcggtt
      721 actccgccca ggagaatacc ttcagccaga tgggcggaca gggcaacaac ctcgtgagta
      781 acgggctgcg tgatctggag gacctccccg cgctgacaag cgtctacggc aaccaggtca
      841 ccaccacgct ggtctccgaa ttcgcgcggc tcaattatgc ctataaggat aagtacctgc
      901 tcacaggcac catccgccgg gacggcacat cgaagttcgc acccgaccat caatacggcg
      961 tattcccatc cggttcggtc gcctggcgga taaaggagga gaatttcctg cagaatgtca
     1021 attggctctc cgacctcaag atacgcggca gctacggtca gacgggtaac cagctcaacg
     1081 tgcagccctt tgtctacctg gcccagtata cctcgggccc cggcgccacc aaccagggca
     1141 acaacggcta tcctttcaac aagacctatc agccgggcct gatcctgacc caacaaccca
     1201 actccaacct gaaatgggag gtcgccaaaa tgaccgatat cgccgtggac gccggcttcc
     1261 tggacaacaa actgacggtc accgcggact actatcacaa ggtgtcgagc aacttcctgc
     1321 tctacctgcc gatacccggc cagagtggct ttacttatca ataccagaat gtgggcagta
     1381 tcctgaacga gggcgtcgaa ttgtcggtta actgggcgca tacggccggc gacctgcatt
     1441 ataacatcgg tgtcaacctc actaccatca agaaccggct gctgagcctg aacagtcacc
     1501 tgaacgaagt gcccaatctt gtcaacctgg gcttctccac caccggcacc aacaactggg
     1561 gaacgtacag catgacaaaa gtgggtggcc cggtgggcga gttctatggg tataaaacgg
     1621 ccggcatctt ccagacccaa aaagagatcg acgacctcaa cgccgcggcc gcggccaaat
     1681 acggcgccgg aaccatgtac gagcctacct ccggacccgg cggcagcgca gtgcccggcg
     1741 accgcaaatt cgtcgacctc aaccacgacg gccggatcac ccccgactcc gaccaggtgg
     1801 ccctgggcag cccgatcccc aagatcttcg gcggcatcac cttcgacgga tcgtggaaga
     1861 actgggattt cagcgtgttc ttctatggaa cggcgggtaa caagatcttt aactaccagg
     1921 aacggaccct cgagagcttc gagacatcca ccggctccgt cggcctggag aacatcgggc
     1981 tgaaatacta ccagaatgcc tggacgccca cccatccttc caaccggtat gcccgcatcg
     2041 acgccaacga attcaacacc aacacccgcc cctcggatgt ttacatagag gacggcagct
     2101 atcttcgcct gaagaacctg accatcggct atacccttcc gttgaagacg agcggagcct
     2161 tcgcccccag gatcagggtc tatataacgg cacagaacct gttcaccatt accaaatata
     2221 ccggcgccga tccggagatc gggcagcccc agggcaccga tgtacaggat atgtcggtga
     2281 tcggcacttc cgcgcgcggc agttcggtgc gcagcacgac cgcctcgggc atcgacgttg
     2341 gcacctatcc caattccagg ttctatatcc tcgggttcaa tgtgaccttt taatccttaa
     2401 caacacaaat caaaagacat gaaaaataaa aggaacatta tttgggtggc ggtattggtg
     2461 ttgctggttc ccctggcctg ttcccggaac ttcctgaacc cgtcggacac gcaaagcgcc
     2521 aacggcagtt cgctgttcca taccccacag gatgcgataa gcctggtcaa tgcgatctat
     2581 gacgggttcc agcacgataa ccagaatttc gtgctgaagg ccctctggta taacgccaac
     2641 tatacgacac aggatttctt caactggggt gcggacgtct cttataacac ctaccagttc
     2701 ccgacggact tcgcatcgct gccggtctac tggaacgatt cctaccaggg catcgccagg
     2761 gccaactcgg ccttccccgt tatcgcgcag atgcgcaccc agggagtcct caccgactcg
     2821 ctggccaact tcctgctcgg ccaggcgtat ttcctgcgcg gggtatacta ttactatctt
     2881 gcctgtacct ttggtggtgt tcccctcgaa ctgaatgtaa ccaccgatgg tttccatccc
     2941 cggtcgaccc aggactcggt gttcatgcgg gtggtaaagg acatggattc cgcctccctg
     3001 ttgctgccct ggccgcaaca gctggccgcc ggcgacctcg gaagagcgac aaaaggcgcc
     3061 gcactgggct ataagggggc tgccgagatg tggttaaagg actataagga cgccatcacc
     3121 gacttccagg ccctgatccc acactatacc ctgctgccca atttcatgag catccatgaa
     3181 tacagcaacc agaacaacgc ggaatcgctt ttcgagctgc agttccacct ccccagcagc
     3241 agcacggtac ccgactggtc gtataacaat aacgaggtga cctggcagtc ttccttcatg
     3301 tggccctggg aaacctccaa cttcggctat acctacgcaa acaagaaact gtacacttcc
     3361 ttcgaagccg gcgacctccg caaaccggca acgatcatcg gtcccgatga ctccatccaa
     3421 agccccggca tcgtggccat cggcgggata cgaaattata gtttcgtgat ccagggattc
     3481 cagggaaaat tatcccttcc ggcctcccat ttcaccggca cggatggaaa gatcatgaac
     3541 acctgcggca agaccggcga tctgtggaca ggcgatcagc cgggtcagcc gagatcgggc
     3601 tactatccca tgaaatactg gcgggacccc aatgtaagcg gtaactcccc aagccagatc
     3661 gacccgcagc ataccaacca catcttcggc gaccagaaca ttaccctgct ccggctcggg
     3721 gagatctatc tcgacctggc ggaatgtttc tacaagaccg gcgatcagac ggacgccacc
     3781 aatgccctga tgaccgtcag gaaccgcgcc tggggcggca gcgcccccgc ttctcccttt
     3841 ggaccggatt tcatgcagat catgctgcag gaataccggc acgagctggg cggagagatg
     3901 tccacctggt ataatatgcg gcgcaccggc ctgcagatcc agtatatcca gaataccttt
     3961 ggcatcacca ttccacccgg tcacgatctt ttgccgatac cgcaatcggc gatcgccacc
     4021 aatccgttcc tgaagcagaa cccaggttac taaatgaaag gacattatct atcattcctg
     4081 cttgccgcat cgatattttg ttcctgccga aacgggaccc tttttgaaag ggtcccgtcc
     4141 tcgcattcgg gcatcacctt tagcaatacg atcgtcgaga ccgatacgat caacccgctc
     4201 gatgtggtca atatctacaa cggcggcggt gtcggtatcg gggatttcaa caacgacggg
     4261 ctgcccgata tctatttcgg cggcaacagg gtgccatccc gcctctatct caacaaaggg
     4321 aatttcacct tcgaggatat cacggaaaag gcaggcgtcg ccgggctggg gagatgggca
     4381 agaggcattt cggtcgtcga tatcaacaac gacgggctac cggacatcta tatctgcaat
     4441 accatctacc gggactccct cctccgccgc aatatcctgt acgtcaacca ggggatcgac
     4501 aaggacggca tcccgcattt ccgggacatg gccgcggaat acgggctgga cgcacactac
     4561 cagtcgacca tggccagttt cttcgattat gataacgacg gcgacctgga tgtatatatc
     4621 acggtgaatg aagcctacaa cggggacgcg cccaccacct tcctccgccg cagcgaccgg
     4681 accgcggctc ccagcagggg aagactgttc cgaaacgact gggacagcac ccttggccac
     4741 ccggtattcc atgatgtttc cgaccaggcg gggatcagct tcgagggcta tggccatgcg
     4801 gcaaccgtct gcgacatcaa caacgacggc tggaaggata tctatgtctc ggatgacttc
     4861 ctgtcgaaca atatcctcta tatcaacaac cataacggaa cgttcaccaa ccacacccgg
     4921 gattacctga aacacacatc ctacaattcg atgggccagg atgtggtgga catcaacaac
     4981 gacggtctcc ccgatatcgt cgagctggac atgaatcccg aggacaatta ccggaagaag
     5041 atgatgctcg cttcggagag ctatcttacc taccagaact tcgaccagtt cggttacgaa
     5101 ttccagtatg tccgcaatac cctgcaattg aaccggggcc ggcagctggg cgagggtgat
     5161 tcactgggca tccccgcctt cagcgagatc gggtatatga gcggtatttc gcaaaccgac
     5221 tggtcctggt cgccgctggt agccgatttt gacaacgacg gctatcgcga tatcatcgtg
     5281 accaacgggt tcccccggga tgtctcggat cacgatttca tggcctaccg ggaagaacac
//
LOCUS       DATGFG010000490         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_129195, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000490 DATGFG010000000
VERSION     DATGFG010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129195"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VKQ52_09095"
     CDS             <1..790
                     /locus_tag="VKQ52_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87385.1"
                     /translation="FSRISFLYGSVSFRLFIEELNQELAFEIENADIRPEFDAIREYF
                     IKALKKKLAVVDITVRYTDHSIISATAKSEDISNINDSLIESVRFEFVRKEIFRPKDG
                     LIINRTNTFDTLLDQFHSSAKELFGTEQDLLNDILNVRKCKHYQQLKYLASKHESSIL
                     KLRFVLQPFSFLFLVSGERKYHIIWETLDTEEATYIWATEKSREALRAILNQIEEIIT
                     GIRKDGRQEYLKQDHLNFSRILHDYSDSKKGFITWKGTLDERLI"
     gene            complement(1021..1521)
                     /locus_tag="VKQ52_09100"
     CDS             complement(1021..1521)
                     /locus_tag="VKQ52_09100"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLZ87386.1"
                     /translation="MERTIRVRSHLPYKPELVWLALTDAGVLGKWFMDNDIKPELNHA
                     FTFRMAPQKGWDGITHCRILEVEYLRRLAYSYQGEAGGEKALACAGIHSETADKAVKG
                     IFARLDTVLSFTLTPIGGGTTLTMEHSGYKGFKLVVVSLIMGMGWKKQLRKKLPGVLE
                     RMVEGG"
     gene            complement(1640..>3420)
                     /locus_tag="VKQ52_09105"
     CDS             complement(1640..>3420)
                     /locus_tag="VKQ52_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87387.1"
                     /translation="LNEEEYVRINNRLQTEVQGRRQAIKTLKEAIARMDEPHSHATPG
                     GPDEDDLNRTLAKLEDQLARRRRMEADLIEAREQAEQFARSKDNFLANMSHEIRTPMN
                     AVLGMAGQLAKTNLDDKQRFYLDTIHSASENLLVILNDILDLTKIRAGKLALEKIGFQ
                     PSAVIGRALRVMMHKAEERGLLLRIAVCDEGLSRVLIGDPYRLNQVLLNLLSNAIKFT
                     SAGGVEISCKVLADTALSQKIVIEVTDTGMGMDESFIQNLFEKFTQEYDSVTRKYGGT
                     GLGMSICKELIELMGGEIRVASEKGIGTMVSLIIELEKGTDRDLSSRDSAPVNTETLQ
                     NKRVLVVDDNDMNRLVATTILGNYGTVIFEAANGQEAVDFLRGQEVDIVLMDIQMPVM
                     SGLEATQVIRREISASLPIIALTANAIRGENQKCLAAGMNDYLSKPFEEDAFIKMISE
                     WIVRQPMQADAGAAGPAAGDARESVPEPGRAALYDLHRLEEIGQGNPDFIRKMVGLFT
                     DQIPAAIDELTAAAANRDFQTVHEKAHLLKPILHNLSIDSLHKDIRELESLALTGTGA
                     DRIDQLVKTIAAIVGAVVEELRTMVF"
BASE COUNT          811 a         1018 c          760 g          831 t
ORIGIN      
        1 attttccaga attagtttct tatatggaag cgtatcgttc cggttattta tagaagaact
       61 gaaccaagaa ctggccttcg aaattgaaaa cgccgacatc cgtccggaat tcgacgcgat
      121 ccgggaatat tttattaaag ccttgaagaa aaaattggcc gtagtagata ttacggtccg
      181 gtatacagac cattcaatca tctccgcaac tgccaaatcg gaggatattt cgaatattaa
      241 tgattcactt atcgagagtg tacgctttga gttcgtcaga aaagagattt tcaggccaaa
      301 ggacggtctg ataataaata gaaccaatac ctttgacacc ttgctggatc agtttcattc
      361 ctccgctaag gagttatttg gtacggaaca ggacttgcta aatgatatct tgaatgtcag
      421 gaaatgcaaa cactatcagc aattgaaata cttggcatca aagcacgagt catccatact
      481 aaaactccgg ttcgttctgc agccgttctc cttcttgttc ctggtatccg gcgaaagaaa
      541 atatcatata atctgggaaa cactcgacac tgaagaagcc acatacatct gggctacgga
      601 aaaatcgaga gaagccttac gggccatatt aaaccagatt gaagaaatta tcaccggcat
      661 acggaaagac ggcagacaag aataccttaa acaggatcat ctcaatttta gccggatatt
      721 gcatgactat tccgattcca aaaagggatt tattacctgg aaaggcacgt tggacgagcg
      781 attgatctaa aattacccta tcttcacctc agctctttcc ccggtcgcaa ttcggtcgcc
      841 ccgaaaccgc caccctcata acgcattgta tctaaagcat aaacgcgtca ccgttcgagt
      901 cccgtccggt ccgcaagcac aaacgcaaca gtgcccagcc tttggctggg catttttcat
      961 tttcacccct ccccctatct cttcttcctg aaattcccaa aatccaacac cacggccccc
     1021 tcaacctccc tccaccatcc tttccagcac tcccggcaat ttcttcctca actgcttctt
     1081 ccaacccatg cccatgatca aactgacgac taccagtttg aaacccttat acccggaatg
     1141 ctccatcgta agcgtagtgc ccccacctat cggcgtcaac gtaaaactca aaacggtatc
     1201 gagcctggca aagatccctt ttactgcctt gtctgccgtc tccgagtgga tgcccgcgca
     1261 agccagcgcc ttttcaccgc cggcctcccc ctgatagctg tatgcgagac gtctcaggta
     1321 ttcgacctcc aatatcctac aatgcgtaat accatcccag cctttctgag gtgccattcg
     1381 gaaagtgaac gcatgattca actccggttt gatgtcgttg tccataaacc actttccaag
     1441 cactccggca tcggttaacg caagccacac caattcgggc ttatacggca aatgactcct
     1501 gacgcgaatg gtacgttcca tttttctgtt ttgatcctgc tattcactat ttttttggga
     1561 aaaccggaaa aaccgagaaa ccgggaaaac cgagaaaccg gaaaaccggg aaatcgggaa
     1621 aaccgggaaa cctgcccggt caaaacacca tcgttctcaa ctcctccacc accgctccaa
     1681 ctatagccgc gatcgttttc accaactggt cgatgcggtc ggcaccggtc ccggtaagcg
     1741 caagactctc cagctcacgg atatccttgt gcaaagagtc gatagaaaga ttatgcagga
     1801 tgggcttcag caggtgcgcc ttctcatgca ccgtttgaaa atcccggttg gcggccgcgg
     1861 ccgttaattc atctatggca gccgggatct gatcggtgaa aagacccacc atcttcctga
     1921 taaaatcggg attaccctga ccaatctcct ccagccggtg gagatcgtaa agcgctgccc
     1981 gccccggttc cggcactgac tcccgggcat caccagccgc cgggcccgca gccccggcat
     2041 cggcctgcat tggttgccgg acgatccatt cggagatcat tttgataaag gcgtcttctt
     2101 cgaaaggctt ggagagatag tcgttcatcc ccgccgccag gcatttctgg ttttctccgc
     2161 ggatagcatt ggccgtaagc gcgatgatcg gaaggctggc gcttatctcc cgccggatca
     2221 cctgtgttgc ctccagcccg ctcatcacgg gcatctgaat atccatcaac acgatatcca
     2281 cctcctggcc gcgcaggaag tcgaccgctt cctggccatt ggccgcctcg aaaataaccg
     2341 tgccataatt gccaagaatg gttgtcgcca ccaggcggtt catatcattg tcatccacga
     2401 ccagcaccct cttgttttga agggtctccg tattgacggg cgcgctgtcc cgcgacgaaa
     2461 ggtcacggtc cgtccctttc tccagctcga tgataaggga caccatcgtc ccgatcccct
     2521 tctcgctggc gacgcggatc tcgcctccca tcagctctat cagctcctta cagatgctca
     2581 ttccaagacc cgtgccccca tacttgcgcg taacggagtc gtattcctgc gtaaatttct
     2641 cgaacaggtt ctggataaaa ctttcgtcca tccccatccc cgtgtcggtt acttcgataa
     2701 cgatcttttg ggaaagcgcg gtatcggcca gcaccttgca ggagatctcc actcctcccg
     2761 ccgaagtgaa tttgatcgca ttgctcagca ggttgagcaa cacctggttc agccggtacg
     2821 gatctcctat caataccctg gacaaaccct catcacatac cgctatccgc aggagcagcc
     2881 cccgttcctc cgccttatgc atcattaccc gcaatgccct gccgatcacg gcggaaggtt
     2941 ggaacccgat cttctcgagg gccagtttcc ccgccctgat cttcgtcagg tcaagaatat
     3001 cgttgagtat caccaggagg ttctcggaag ccgaatgtat cgtgtcgaga tagaaacgtt
     3061 gcttgtcgtc caggtttgtt tttgccagtt gtcccgccat tcccagcacc gcgttcatcg
     3121 gcgtgcggat ctcatggctc atattagcga gaaaattatc ctttgacctg gcaaactgct
     3181 cggcctgttc ccgggcttcg atcaggtcgg cctccatccg ccgtcgccgg gccagctgat
     3241 cctccagttt cgcaagcgtg cggttgagat catcctcatc cggtccgccg ggcgtagcgt
     3301 gagaatgcgg ctcgtccatc cgggcgatgg cctccttgag ggtttttatg gcctgccgcc
     3361 ggccctgtac ttcggtctgc agcctgttat tgatacggac atactcctcc tcgttaagcc
//
LOCUS       DATGFG010000491         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1615448, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000491 DATGFG010000000
VERSION     DATGFG010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615448"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VKQ52_09110"
     CDS             complement(<1..1266)
                     /locus_tag="VKQ52_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87388.1"
                     /translation="MTTTTTTATTSITGTAAPTPRRLRLFRLIALILPILLLILTEAL
                     LHLFHYGYDTSVFITAPGNNDYLVFNPDASKQYFSDPLLATSGNRELFHKTKQPGTLR
                     IFVLGESTTIGYPYFHNGSFHRWLQYRLMRTNPGKKIEIINVSLTAVNSYTVLGFARE
                     VVKQEPDAVLIYTGHNEFYGALGVASTSRLGSTPWLINTLLWLRQWRLFQLLTRIFHA
                     LTHLFSTGPPKTGHTRMELMVADQQIAFGSPLYASGIRQFRTNMDETLALFHHSGIPV
                     FLSNLVSNLKDQRPLNSFTTTHLAADPTHPDADPTNTGADSAHPDPANALYNFTLAGQ
                     AWQRGDYPTAKQDYILAKDLDALRFRAPETLNDIIDSLCKRYRENTFLVDTRAAFEAA
                     SDHGIIGNSLLLEHVHPNLTGYALISDVFY"
     gene            complement(1277..>3103)
                     /locus_tag="VKQ52_09115"
     CDS             complement(1277..>3103)
                     /locus_tag="VKQ52_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLZ87389.1"
                     /translation="AHGVDMLLAPVLCLPRDPRWGRVEETYGEDTWLDARNGVAMVKG
                     LQGNGVDKPDAVISEPKHFAVHGIPEAGSNTAPVNIGEREARTSFLYVFEKAVREGGA
                     LSMMAAYSELDGIPCVDNKWLLTDVLRKEWGFKGFVLSDLGAIKMSFENHHTVATPAE
                     ALARSLKAGLNMQFYDWPHAEFRSAMHTAIQQHLLTLPQLDNAVRDILRVKFLLGLFD
                     HPYTDTTRIAAVFHTVAHQQLALKAAAESICLLKNDDHLLPIKNNIHTIALIGPLATS
                     TYLGGYSNGEGKAISVLDGLRQRAGDRLTIRYEPGYTAGDAPSVAPAPASANAPGNPT
                     DTARHSPTDPLTAALDAVHGSDLTIVALGEEPAIVGEGKDRAHLELSDQQANLIKAIV
                     ATGKPVVALLSNGRPLCIDWVAGHVPAILETWFSGEQGGLAIADILLGNTNPSGRLPM
                     TFPCSEGQIPFYYNHKPTSGHRYVDEKDTPLFPFGHGLSYTHFDYTDLKISPATIPVG
                     GSADIRVTVTNTGSLAGDEITQLYIRDLVSSVTTPAIALKGFARTSLAPGESKTLHFR
                     LGPDQLALWNTEMKRVVEPGEFKIMIGASSTDIRLTASLWVK"
BASE COUNT          527 a          636 c         1263 g          677 t
ORIGIN      
        1 gtagaaaacg tcggagatca gcgcatagcc ggtgaggttg ggatggacgt gttcgaggag
       61 gaggctgttg ccgatgatgc cgtggtcgga ggcggcttcg aaggcggcgc gggtgtcgac
      121 gaggaaggtg ttttcgcggt atcttttgca gaggctgtcg atgatgtcgt tgagggtttc
      181 gggggcgcgg aagcgaaggg cgtccaggtc ctttgcgagg atatagtcct gtttggccgt
      241 gggatagtcg ccgcgttgcc aggcctggcc ggcgagggtg aagttgtaca gggcgtttgc
      301 cgggtcggga tgggcggagt cagcgcctgt gttggtggga tcggcgtctg ggtgagtggg
      361 atcggcggcg aggtgggtgg tggtgaaact attgagcggc cgctggtcct tgagattgga
      421 gacgaggtta ctgaggaaga ccggaatgcc gctatggtgg aacagggcga gggtctcgtc
      481 catgttggta cggaactgcc ggataccgga ggcgtagagc ggggatccga aggcgatctg
      541 ctggtcggcg accatgagct ccatgcgggt gtggcccgtt tttggcggac cggttgaaaa
      601 gagatgggtc agggcgtgga atatacgggt gaggagttgg aagagacgcc actggcggag
      661 ccagagaagg gtgtttatga gccagggagt gctaccgagg cgagaggtgg aagctacgcc
      721 gagggcgccg tagaattcgt tgtgcccggt gtagatgagg acggcatcgg gttcctgttt
      781 taccacttcg cgggcgaagc ccaggacggt gtaggagttg acggcggtga gagagacgtt
      841 gatgatctct atttttttcc cggggttggt gcgcatcagc cggtattgga gccagcggtg
      901 gaaggagccg ttgtggaagt acggatagcc gatggtggta gattcaccga ggacgaagat
      961 gcgaagggtg ccgggttgtt ttgtcttgtg gaagagttcc cggttgccgg aagtggccag
     1021 gagcgggtcg gaaaagtatt gttttgatgc atccggattg aagacgaggt agtcgttatt
     1081 gccgggtgcg gtgatgaaga cggaggtgtc atagccgtag tgaaagaggt gcaggagcgc
     1141 ctctgtcagg ataaggagca ggatgggcag gatgagggcg atgagacgga agaggcggag
     1201 acgcctgggc gtgggggctg cggtgccggt gatgcttgtg gtggcggtgg ttgtggtcgt
     1261 cgtcatggtt ggcggtttat ttgacccaga gactggctgt gaggcggata tcggtggagg
     1321 aggcgccgat catgatcttg aattcgccgg gttccactac tcttttcatt tcggtgttcc
     1381 agagggctaa ctgatcgggg ccgaggcgga agtggagggt tttgctttcg ccgggcgcca
     1441 ggctggtgcg ggcgaagcct ttcagggcga tggcgggtgt cgtgacgctg ctgaccagat
     1501 cgcggatata caattgggtt atttcgtcgc cggcgagaga accggtgttg gtgacggtga
     1561 cccggatgtc ggcgctgccg ccgacgggga tggtggcggg acttattttg agatcggtgt
     1621 agtcgaaatg tgtatagcta agcccgtggc caaaggggaa caggggtgta tccttttcgt
     1681 cgacgtagcg atgaccgctt gtcggtttgt ggttatagta gaagggtatc tggccttcgg
     1741 agcaggggaa ggtcatgggc aggcggccgg aggggtttgt attaccgaga aggatgtcgg
     1801 cgatggcgag gccgccctgt tcgccggaga accaggtctc gaggatggcg gggacgtgtc
     1861 cggccaccca gtcgatgcag aggggccggc cgttggagag gagggcgacg acgggtttcc
     1921 cggtggcgac gatggctttt atgagatttg cttgctgatc gctgagttcg agatgtgcgc
     1981 ggtcttttcc ttcgccgacg atggcgggtt cttcgccgag ggcgacgatg gtgaggtcgg
     2041 acccgtggac ggcgtcgagg gccgcggtga gggggtcggt tggggagtga cgggcggtgt
     2101 ccgttggatt gccgggtgcg ttcgccgatg cgggggcggg ggcaacggag ggtgcgtcgc
     2161 cggctgtgta gcctggttcg tagcgtatgg tgagccgatc gccggcgcgt tggcggaggc
     2221 catcgaggac ggagatggct ttgccttcgc cgttggaata gccgccgagg taggtgctgg
     2281 tggcgagggg gccgatgagc gcgatagtat ggatgttgtt ctttatgggg aggaggtgat
     2341 catcgttctt caggaggcag atgctttcgg ccgcggcttt cagggcgagt tgctggtggg
     2401 caacggtgtg gaagacggcg gctatgcggg ttgtatcggt ataggggtga tcaaagaggc
     2461 cgaggaggaa ttttacgcgg aggatgtcgc ggacggcgtt gtcgagttgc gggagggtga
     2521 ggaggtgttg ttggatggcc gtatgcattg ccgagcggaa ttccgcgtga ggccagtcat
     2581 agaactgcat gttgagaccg gctttcagcg atcgggcgag agcttcggca ggtgtggcga
     2641 cggtgtggtg attttcgaag gacattttta tggcgccgag gtcggagagg acgaagcctt
     2701 tgaagcccca ttccttgcgg aggacgtcgg tgaggagcca tttgttgtcc acgcagggga
     2761 taccgtcgag ttcgctatag gcggccatca tggagagggc gccgccttcg cggacggctt
     2821 tctcgaagac gtagaggaag gaggtgcggg cttcgcgttc gccgatattg acgggggccg
     2881 tgttgctgcc tgcttcgggg atgccgtgga cggcgaagtg ttttggttcg gagatgacgg
     2941 catcgggttt atcgacgccg ttgccctgga ggcctttcac catggcgacg ccgttacggg
     3001 catcgagcca ggtgtcttcg ccataggttt cttcgacgcg gccccagcgg gggtcacggg
     3061 gcaggcagag gacgggggcg aggagcatat cgacgccgtg ggc
//
LOCUS       DATGFG010000492         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_350595, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000492 DATGFG010000000
VERSION     DATGFG010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350595"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VKQ52_09120"
     CDS             complement(<1..363)
                     /locus_tag="VKQ52_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87390.1"
                     /translation="MKKSLKVAHILSWFNLFFWGIPLVINLLNTLLAFNPLILGAVVL
                     FCSIPLHSYAALQLHKSIRRPEVKLSHNTPAGIRFVGLIALFFGFFFLVTGMAVARNT
                     SMLLDMLREQGPYKDMYAG"
     gene            complement(360..2201)
                     /gene="aspS"
                     /locus_tag="VKQ52_09125"
     CDS             complement(360..2201)
                     /gene="aspS"
                     /locus_tag="VKQ52_09125"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HLZ87391.1"
                     /translation="MYRTHTCGELRSAQIGQTVTLAGWVQTLRKFGSITFIDLRDRYG
                     ITQLLFGENLNKELTDNPLGREFVVQVKGKVTERTNKNPQLATGDIEITVQSFTILNK
                     AVTPPFTIQDDTDGGDDLRMKYRYLDLRRAAVRKNLELRYAVNRAARNYLHENHFMDL
                     ETPFLIKSTPEGARDFVVPLRMNPGQFYALPQSPQTFKQLLMVSGYDRYYQVVKCFRD
                     EDLRADRQPEFTQIDCEMSFVEQEDILAMFEGMIRSIFKTVKNIDYTEEIARMTWEDA
                     MWQYGNDKPDIRFGMPLLNLKSAFTKGGAIVARGELINGAGFGVFDNAETVLAIAVPG
                     CSEYTRKQTDELTDWVKRPQIGMQGLVYIKYNTDGTLKSSVDKFYGEEKLKAIAAAAG
                     AQPGHLILVLAGREERTRKAMSELRLEMGRRLGLRREDEFKLLWVLDFPLFEYAEDEN
                     RWVARHHPFTSPKPSDIRVMINNSPVIANPEKYLEHPYNHIKANAYDMVLNGNEIGGG
                     SIRIYQRELQEKMFAALGMTPEESMHKFGFLLGAFEYGAPPHGGIAFGFDRLCAILGG
                     SESIRDFIAFPKNNAGRDVMLDAPSTIDEKQLTELSIRLDTSGRDSL"
BASE COUNT          485 a          695 c          571 g          496 t
ORIGIN      
        1 cccggcatac atatccttgt aaggcccctg ctcccggagc atgtcaagca gcatcgacgt
       61 gttcctggcc acggccatcc cggtcaccag gaagaaaaac ccgaagaaca gggcgataag
      121 ccccacaaaa cggatacccg cgggggtatt atggctgagc ttcacctccg gtcgccggat
      181 gctcttgtgc agctgcagcg cggcataact gtgaagcggg atcgaacaaa agagtaccac
      241 cgcccccagg atgagcgggt tgaaggccaa caatgtattc aacaggttga tcacaagcgg
      301 gatcccccag aagaacaggt tgaaccagga aaggatatgg gcgactttca gcgatttttt
      361 cataagctgt ccctcccgct cgtatccaac cggatcgaca attccgtcag ttgtttctca
      421 tcgatcgtac tgggtgcatc cagcatcacg tcccttccgg cgttgttctt cgggaaggcg
      481 ataaaatccc ggatgctctc gctgccgcca aggatcgcgc aaagccggtc aaagccaaag
      541 gcgatacccc catgcggcgg cgcaccatat tcaaaggcgc ccagcaggaa gccgaacttg
      601 tgcatggact cctccggggt catccccagc gcggcaaaca tcttctcctg cagctcccgc
      661 tgatagatgc ggatcgagcc gcccccgatc tcattcccgt taaggaccat gtcataggcg
      721 ttcgccttga tatgattata gggatgctcc aggtatttct ccggattcgc gatcacgggg
      781 ctgttattga tcatcacccg gatgtcggaa ggcttcggcg aagtaaaagg atggtgacgg
      841 gctacccagc gattctcgtc ctcggcatat tcgaacaggg gaaagtccag cacccacaac
      901 agcttgaact cgtcctcccg gcgtaatccc aggcgccggc ccatctccag gcgtaactca
      961 ctcatcgcct tgcgcgtccg ctcctccctg ccggcaagca cgaggatcag gtggccgggc
     1021 tgcgcacccg cggccgcggc aatggccttc agcttctctt ctccatagaa tttatcgaca
     1081 ctgctcttca gcgtcccgtc ggtgttgtat ttgatataca caagaccctg catcccgatc
     1141 tgcggccgct ttacccagtc cgtcagctca tcggtctgct tgcgggtata ttcactgcaa
     1201 cccggaaccg caatggccag caccgtctcc gcattgtcga acaccccgaa cccggcgcca
     1261 ttgatcagct ctcccctggc gacgatggcg ccgccctttg taaaggccga cttcaggttc
     1321 agcaagggca tcccgaaccg gatatccggc ttgtcattac catattgcca catggcatcc
     1381 tcccaggtca tccgggcgat ctcttcggta tagtcaatgt tctttacggt cttgaaaata
     1441 gaccggatca tcccttcgaa catcgccagg atgtcctcct gctcgacaaa actcatctca
     1501 cagtcgatct gcgtgaactc gggctgccgg tcggcacgca ggtcctcgtc ccggaaacat
     1561 ttgaccacct ggtaatagcg gtcataaccg ctcaccatca gcaactgctt gaaggtttgc
     1621 ggcgactgcg gaagcgcata gaactggccc gggttcatcc gcaacgggac gacaaaatcc
     1681 cgtgccccct ccggcgtcga tttgatcagg aacggcgttt ccaggtccat aaaatggttc
     1741 tcatggagat aattacgggc cgcccggttc acggcatacc gcaattcaag gttcttcctc
     1801 accgcggccc ggcgaaggtc caggtaccgg tatttcatcc gcagatcgtc gccgccatcc
     1861 gtatcatcct ggatggtaaa cggcggcgtc acggccttgt tcaggatcgt aaaggattga
     1921 accgtgatct cgatatcccc cgtcgccaat tgtgggttct tgtttgtccg ctcggttacc
     1981 ttacccttca cctgtacgac gaattccctg cccagggggt tgtctgtgag ctccttattc
     2041 aaattttcgc caaacagcaa ctgcgtgatc ccatagcggt cacgcagatc gataaaagta
     2101 atacttccga atttccgaag ggtctgcacc catcccgcca gggtaacggt ctggccgatc
     2161 tgcgcgctcc ttaattctcc acaggtatga gtccggtaca ttccttattt tttgagcgcc
     2221 aaaggtatgc aggttgcggg aaaaatt
//
LOCUS       DATGFG010000493         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_349938, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000493 DATGFG010000000
VERSION     DATGFG010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349938"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2474)
                     /locus_tag="VKQ52_09130"
     CDS             complement(<1..2474)
                     /locus_tag="VKQ52_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HLZ87392.1"
                     /translation="MYMIGQVAALKNDIISLAELHRMVGEDSIGYIREAFAGTSPGEG
                     SGSLEVAIVGMACIYPGARNIDEFWRNIVLGKDAVTEVPDERWNKEIYYDPSATGGDK
                     SASKWGGFLPVVDFDPVEFGIPPQSLAAIEPAQLLSLLVAREALKNAGYADREFDREN
                     VSVIMAADGGNDLENLYRFRGLFPQLLGEMPKELDAALPRLTEDSFPGVLANVIAGRI
                     CNRLDLGGRNFSVDAACASSLAAVDLACQELQLGKADMVLAGAADLHNSINDYLLFSS
                     TRALSKTGKCMTFDSAADGIALGEGVAMLVLKRYEDAKRDGDTVYAIIKGVGGSSDGK
                     SLGLTAPRKKGQRKALERAYEQAGLSPAEAGLIEAHGTGTVVGDQTELSALSDLFLQS
                     GAIAGQTSLGSVKTQIGHTKCAAGLAGLIKAALSVYHGVKPPTLHIKAPNRGYAPRLS
                     PFRFDAEAGPWLEPSRIAGISAFGFGGTNFHVVIASESRMGISDRKAPPVIQSWPAEL
                     FVFRGDDYEEARGVLATCRSILLNNDKLSFIDLAYSLAVRNGKDVQLSIVADTVEDLL
                     LKMDLALSGAVSDGIFVREEKEGKVAFLFPGQGSQRVNMARSLLTIFPAMREGLLSNP
                     EYGRVLYPHSVFDEGSLRQQKDEIRDTRLAQPLLGIVDLAIAGFLRDLGIEPAMVAGH
                     SYGELPALCFAGAFGPQDLVPLSESRARAILDATGDDKGIMIALNCEPGMLNQLTGEG
                     SGIYAVNHNSPEQWVLAGATGDMQVLMEKLKAQEIWFSQLGVACAFHSPLVAGARLVF
                     EEAMKDVVFDPPSVAVWSNTTASVYPP"
BASE COUNT          547 a          835 c          603 g          513 t
ORIGIN      
        1 ggcggataca ccgaagccgt ggtattcgac catacggcca ccgaaggagg atcaaagaca
       61 acatccttca tcgcctcttc aaaaaccaat cgcgcacccg ccaccagcgg actatgaaag
      121 gcacaggcca cgcccagttg cgagaaccat atctcctggg ccttcaattt ctccatcaat
      181 acctgcatgt ccccggtcgc acccgccaaa acccattgct ccggggaatt atggttcacc
      241 gcatagatcc cgctcccctc accggtaagt tggttcaaca tcccgggctc gcaattcaac
      301 gctatcatta ttcctttgtc gtcacccgtc gcatccagga ttgcccttgc cctgctctcg
      361 ctcagcggca ccaggtcttg cggaccgaat gccccggcaa aacacagcgc cggcagttcg
      421 ccatagctat gccccgcaac catggccggc tcgattccca gatcccgcaa aaaaccggca
      481 atcgcaagat ctactatccc cagcaagggt tgtgcaagcc gggtatccct tatctcgtct
      541 ttttgttgtc gaagcgatcc ttcgtcaaag acgctgtgcg gataaagcac ccgtccatac
      601 tccgggttgg atagcaaccc ttcccgcatc gcagggaaga tcgtcagcag gctcctggcc
      661 atattgacac gctggcttcc ttgtcccggg aatagaaaag ctaccttgcc ctccttctcc
      721 tcccggacaa aaatgccgtc ggagacagcg ccggataagg caagatccat cttcagcagc
      781 aggtcttcca ccgtatcggc cactatcgac agctgaacat ccttcccatt gcgcaccgcc
      841 agactgtaag ccaggtcgat aaaagagagc ttatcattgt tcaggagtat cgaccggcag
      901 gtggcaagga cacccctggc ctcttcgtaa tcgtctcctc tgaatacaaa tagttcggca
      961 ggccaggatt ggatcaccgg cggcgctttc ctatcggaga tccccatccg ggattcactg
     1021 gcgatcacca catggaaatt ggtccctcca aatccaaaag cgctgatgcc cgcaatccgg
     1081 gaaggctcga gccaagggcc cgcttccgca tcaaaccgga agggactcag ccggggggca
     1141 taaccccggt tgggtgcttt tatatgaagg gtcggcggct ttaccccatg atacactgat
     1201 agcgcagcct tgatcaatcc cgccagaccc gcggcacact tcgtatgccc gatctgggtt
     1261 ttcacggaac ccaggctggt ttgcccggcg atcgcccccg attggaggaa taggtcggat
     1321 aaagcgctga gttccgtctg atcccctacg acggtaccgg tgccatgcgc ttcgataagc
     1381 cccgcttccg ccggcgaaag accggcttgt tcataagccc gttccaacgc cttcctttgc
     1441 ccttttttcc tcggcgccgt cagtccaaga ctcttgccat cgctcgatcc gcccacaccc
     1501 ttgatgatcg catagaccgt atccccatcc cttttcgcat cctcataccg tttcaacacc
     1561 agcatggcca ccccctcgcc caatgcgatg ccgtcggcag cggaatcgaa ggtcatgcac
     1621 ttgcccgttt tggacagcgc ccgggtgctg gagaaaagca ggtagtcgtt gatgctgttg
     1681 tgaaggtccg ccgctccggc cagcaccata tccgctttcc caagctgcag ttcctggcag
     1741 gccagatcca cggcggcaag agaggaagca caggccgcat ccacgctgaa attccgccca
     1801 cccaggtcaa gcctgttgca aatacggccc gcgatcacat tagccagcac accagggaaa
     1861 gaatcctcgg tcagcctcgg caaagccgca tccagttcct tcggcatctc accgagcagc
     1921 tgcggaaaaa gcccgcggaa ccggtagagg ttctcaagat cattgccgcc gtccgccgcc
     1981 ataatgaccg acacattctc ccggtcgaat tccctgtccg cataaccggc gtttttcagg
     2041 gcctcccttg caacaagcag gctgagcagt tgcgcaggct cgatagccgc cagggattga
     2101 ggcggtatcc cgaattccac cggatcgaaa tcgaccaccg gtagaaagcc gccccacttg
     2161 gatgcagact tgtcgccacc ggtagccgaa gggtcatagt atatttcttt attccaacgt
     2221 tcatccggaa cttcggttac ggcatccttt ccaaggacta tattccgcca gaactcatcg
     2281 atattcctcg cgccgggata gatacaggcc atgcccacta tggcgacctc caacgatccc
     2341 gacccttctc ccggcgacgt cccggcaaag gcctcccgga tatacccgat gctgtcctct
     2401 cccaccattc tatgcaactc cgcgagagag atgatgtcat tcttcagcgc ggccacctgg
     2461 ccgatcatat acatcccggc cgggaattgc tcctcctc
//
LOCUS       DATGFG010000494         4020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_750527, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000494 DATGFG010000000
VERSION     DATGFG010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750527"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2972
                     /locus_tag="VKQ52_09135"
     CDS             <1..2972
                     /locus_tag="VKQ52_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ87393.1"
                     /translation="GQPLVFSHPQFDAEQRRPKGDTVPPVRLTSRFLRGPEIDSKTSI
                     DKKDTIFIPAEADQTIDVLYEKTNRESFLGSISTVDSKVLEATPASNYVFALQGRLTG
                     VNIAQTSGFQAFSIDSLTHADILGNIPTYTTGAGPSDNSQFTIQLRGHAASSGQSPIA
                     IVDGVQRELYNIDPNTIQSVSILKDPLSNLLLGQNSSRGALIVTTKEPQMGPTRLSIT
                     AESGIQKSLGMPAPLPAYQYAYLLNEALLNDGKNPLYTQADFNAWRSHTDPLGHPDVN
                     WYHTILRSAPMLSRLNLSAAGGSAIARYVISLSYMNDQGIFVNSSANSYSTNAGIQRY
                     LLNSKVDVDVNKYFSLRLQVMGRLQDANQPGVTTGAILNNLLSTPNNAYPVKNPDGSY
                     GGNPNYTQNLLAQTVASGYALDHVRDVVSNLTLNYKLDKVTKGLWVRGGGNISVESET
                     NMNRSLQTSVFQAVVGQDTTYNHYGSSVAQISRYNTGAWARYRYVQLAAGYDRRFGDH
                     SVNGELLFDQKRSLLNYDLPAQLTNFAGKAAYSYKDRYFVQGAANYSGYDRYAPGHQY
                     GLFYAGGVGWDITKEDFFHKIAPWIDHFKLRATYGRTGNANVDYYGYYIWREHFGQSA
                     PNYAMGSTYNFAVQTLSEGGQVGSQFLPNSNATWEKADKFDGGIDLAFFHNRLLLTAD
                     YYHERYFDVMQQRGDNVALIGINYPAENIGRDLYQGLEVELTYQDRIGNVNYFISGNA
                     AAQESKVLFMDEQYEQNPWNVHTGRPVNQLFGYVAQGLFQTAADAASSPSLPGYTQHA
                     GDIKYKDLNGDGVIDQFDLAPIARDRPLVIYGLSAGISYKGIEFSALLQGVANRVAYV
                     DQPYYNYGFLPKGNTYSQAYTPALGRWIPEDVYKASAPRLTAGGNLENGQSLINTFAS
                     SYYVKNGDFFRIKNVNLSYTLPYRWVRRAKFGGVKLFVNAQNLWTWAAFHGIDPEVSL
                     PSYPLQRVVNAGINVKF"
     gene            3000..>4020
                     /locus_tag="VKQ52_09140"
     CDS             3000..>4020
                     /locus_tag="VKQ52_09140"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87394.1"
                     /translation="MKIRFLIPATILAAAITACQKQYEPVPLGEQVTLDYAFDTKDSA
                     GTLALTFLSTCYLEALPNGHNRVGGDYLDAASDDAVSNKLTVSPVQQIATGAYSAASP
                     NADDEWTHNYFAIRDCNIFIKEIYRVPLAEKLPSGRPAIAAYRSEARFLRAWEYFELV
                     KRYGGVPLLGDTVFTITQNAQMPRNSFADCINYIVSECDNIKDSLRTVSDITGASYGR
                     ITKGAAMALKAEALLLAASPLYNGANIDPSNPLTGYTSYDANRWQLAAQAAQDVIGSG
                     VYSLMTDFKAVFITQAQPIGANTESIFWMQVGANNSVEQANSPIGFSSAGAQGNTGPT
                     QNLVDA"
BASE COUNT          903 a         1244 c         1054 g          819 t
ORIGIN      
        1 tcggccagcc cctggtgttc agtcatccgc agtttgacgc ggagcaacgc cgccccaagg
       61 gggacaccgt gccacccgtc cgcctgacca gccgcttcct ccgcggaccg gagatcgact
      121 caaagacctc catcgacaaa aaggacacga tctttatccc ggcggaagcg gaccaaacaa
      181 tcgacgtcct gtatgagaag accaaccggg aaagtttcct gggctcgatc tccaccgtcg
      241 acagcaaggt gctggaggcc actcccgcca gcaactatgt gtttgccctg cagggccgcc
      301 tcaccggggt gaatatcgcc cagacctccg gcttccaggc cttttcgatc gactctctta
      361 cccacgccga tatcctgggc aatatcccca cctataccac gggcgctggt ccgtccgaca
      421 actcccaatt cactattcag cttcgcggcc atgcggcgag ctcgggacaa agtcctatcg
      481 ccatcgtcga cggggtacag cgggaactat acaatatcga ccccaatacc atccagtcgg
      541 tgtcgatcct caaggacccg ctgtccaacc tcctgctggg ccagaacagc tcccgcggcg
      601 cgctgatcgt gacgacgaaa gagccgcaga tgggaccgac gcggctaagc atcaccgccg
      661 agtccggcat ccagaaatcg ctcggtatgc cggcgccgtt gcctgcttat caatacgcct
      721 atcttctcaa tgaggccctg ctgaacgacg gcaagaaccc gctttataca caggccgatt
      781 tcaacgcctg gcgtagccat accgaccccc tgggtcaccc ggatgtaaac tggtatcaca
      841 ccatcctgcg cagcgcgccg atgctgagcc ggctcaatct tagcgccgcc ggcggctccg
      901 cgatcgcccg ctatgtgatc tcgctgagct atatgaacga ccagggcata ttcgttaatt
      961 ccagcgccaa cagctatagc accaatgcgg gaatccaacg gtacctcctc aactccaagg
     1021 tggatgtgga cgtgaacaaa tatttcagcc tgcgcttaca ggtgatgggc cgtttgcagg
     1081 atgcgaatca gcccggcgtc accacgggcg ccatcctcaa caatctcctg agcacgccca
     1141 acaatgctta cccggtgaaa aacccggacg gctcgtatgg cggcaacccg aactatactc
     1201 aaaacctgct ggcacagacg gtggcctcgg gctatgcgct ggaccatgtc cgcgatgtcg
     1261 tctccaacct taccctgaac tataaattgg acaaggtgac caagggactg tgggtgcggg
     1321 gcgggggcaa tatttcggtg gaatccgaga ccaatatgaa ccgtagcctg caaacatccg
     1381 tcttccaggc agtggtcgga caggatacaa cctacaatca ttatggcagt tccgtcgccc
     1441 agatcagtcg ctataatacg ggagcctggg cccgctaccg ttatgtccag cttgcggccg
     1501 ggtatgaccg ccgtttcggc gaccactccg tgaatggcga actgctattc gatcaaaaac
     1561 gttccctgct taactacgat ctgcctgccc agctgaccaa tttcgccggc aaggcggctt
     1621 atagctataa ggaccggtac ttcgtccagg gtgctgcgaa ctatagcggc tatgaccgct
     1681 atgcacccgg tcaccaatat ggtctcttct acgccggcgg ggtgggttgg gatatcacca
     1741 aggaagactt ctttcataaa attgcgccgt ggatcgatca tttcaagctg cgggcgacgt
     1801 atggccgtac cggcaacgcc aacgtagact attatggcta ttatatctgg cgcgagcact
     1861 tcggccagtc ggcgcctaac tatgccatgg gcagcaccta caattttgcc gtacaaaccc
     1921 tctcggaagg cgggcaggtg ggttcgcaat ttctgccgaa cagcaatgcc acctgggaaa
     1981 aggcggataa attcgatggg ggtatcgatc tcgccttctt tcacaaccgc ctgttgctga
     2041 cggcggacta ctaccacgaa cgttatttcg acgtgatgca gcaacgcggg gataacgttg
     2101 ccctcatcgg catcaactat ccggcggaaa atatcggcag ggacctctat cagggtctcg
     2161 aggtcgagct gacctaccag gaccgtatcg gcaatgtgaa ttattttatc tccggcaatg
     2221 ccgcggcgca ggagtcgaag gtgttgttca tggatgagca atacgaacaa aacccttgga
     2281 acgtccatac cggccggccc gttaaccaat tattcggcta tgtcgcgcaa gggctctttc
     2341 aaacggctgc cgatgccgcg agcagtcctt ccttgccggg atatacgcag catgccggcg
     2401 acatcaaata caaagacctc aatggcgatg gcgtcatcga ccagtttgac ctggccccga
     2461 ttgccaggga ccggccgctg gtgatctacg gcctttccgc ggggataagc tataagggca
     2521 tcgaattcag cgcactcttg cagggcgtcg ccaaccgggt agcatatgtc gatcaaccct
     2581 attacaatta tgggttcctc ccgaagggga atacctactc ccaggcctat acgccggctc
     2641 ttgggcgatg gatccccgag gacgtctata aagccagtgc gccgaggctg actgccggcg
     2701 gcaacctgga gaacggccag tccctgatca acacctttgc cagcagctac tacgtcaaga
     2761 acggcgattt cttccgcatc aagaatgtca acctgagtta tactctaccg taccgctggg
     2821 tccgaagggc aaaattcggc ggggtcaaac tatttgtcaa tgcccagaac ctgtggacct
     2881 gggccgcttt ccacggtatc gatccggaag tatccctgcc cagctatccg ctgcagcgcg
     2941 tggtgaatgc gggtatcaat gtaaaattct aattcgtcaa acgattaaac tgattgctga
     3001 tgaaaatccg gtttcttata ccagcgacga tcctggctgc cgccattacg gcctgtcaaa
     3061 agcagtacga gcccgtcccg ctcggcgagc aggtcaccct ggactatgcc tttgatacca
     3121 aggactccgc tggtacgctg gcgctgacct ttctatcgac ctgttacctg gaagcgttgc
     3181 ccaacgggca caatcgcgta ggcggcgact atctcgacgc cgcttcggac gatgccgttt
     3241 caaataagct cacggtatcc ccggtgcagc agatagccac cggcgcctat tccgccgctt
     3301 cgcccaatgc cgatgacgaa tggacccaca actatttcgc catccgggat tgcaatatct
     3361 ttatcaagga gatctatcgt gttcccctgg ccgaaaagct gcccagcggc cggcccgcta
     3421 ttgcggccta tcgttccgaa gcccgttttc tccgtgcctg ggaatatttc gaactcgtca
     3481 aacgctatgg cggcgtaccg ctgctggggg ataccgtctt cacgatcacc caaaatgccc
     3541 agatgccgcg caacagcttc gccgactgta tcaactacat cgtcagcgaa tgcgataata
     3601 tcaaggacag cctccgtacc gtatccgata tcaccggagc ctcctatggc cgtatcacaa
     3661 aaggggccgc catggccctg aaggcagaag cgcttctgct ggcggccagc ccgctttaca
     3721 atggcgccaa catcgatccg tcgaatccgc tcaccggata taccagctac gacgccaacc
     3781 gctggcaact ggcggcccag gcggcgcagg acgtcatcgg ctcgggcgtc tacagcctga
     3841 tgactgactt caaagctgtt tttattacgc aggcgcagcc gataggcgcc aataccgagt
     3901 ccattttctg gatgcaggtg ggcgccaaca acagcgtaga acaagccaat tcccccatcg
     3961 gttttagctc ggcgggtgcg cagggcaata ccggacccac gcaaaatctc gtcgacgcct
//
LOCUS       DATGFG010000495         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_180987, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000495 DATGFG010000000
VERSION     DATGFG010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180987"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..281)
                     /locus_tag="VKQ52_09145"
     CDS             complement(3..281)
                     /locus_tag="VKQ52_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87395.1"
                     /translation="MSVFLERKELFDQVLLLGEEEMQDPIGVFERFFSDYRLHECRYI
                     LWAMVQTCLTTDNTEFGNPEERADLLLRYKDLERLLEAGALILKKPKN"
     gene            complement(421..1617)
                     /locus_tag="VKQ52_09150"
     CDS             complement(421..1617)
                     /locus_tag="VKQ52_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ87396.1"
                     /translation="MSHYQDNHRLFHRHVAQTSPNPLGLEIVKAEGLYLYDKNGKEYL
                     DLIGGISVCNTGHRHPRVVEAIKDQADRYLHLLVYGELIQSPQVEYASLLAAHLPPSL
                     DSVYFTNSGAEATEGAMKLAKRITGRPEIVAFHQSYHGSTQGALSIIGDEYWRNAFRP
                     LLPGIRHVPYNTPAALDAITTHTACVIAETVQAERGVFAPDETWLRQLRQRCDETGTL
                     LVFDEIQAGFGRTGTLWAFQRYNIIPDILLLGKALGGGLPMGAFIAGRERMWTLTGDP
                     VLGHITTFGGHPLCCAAGLASMQVLLGSGWIEAVAQKEALFRRLLVHPAIKAVRSSGL
                     LIAVEFDNFEQNKRIIDTCIANGLLTDWYLFAPHCLRIAPPLSITEAAIRKACTTILS
                     SIDFFH"
     gene            1672..2208
                     /locus_tag="VKQ52_09155"
     CDS             1672..2208
                     /locus_tag="VKQ52_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HLZ87397.1"
                     /translation="MATILPVEGKSPRFGKNCFVAPNATVVGDVEMGDECSIWFNAVV
                     RGDVNCIRIGNKVNIQDGVILHGTYKKASVTIEDCVSIGHNAIIHGCTIRERVLIGMG
                     AIIMDNAEIGKNSIIAAGAVVLEGTKVESGTIYGGIPARKIKDVPADLISGQIDRIAN
                     HYLIYAGWFKDANDAPQP"
     gene            2550..2885
                     /locus_tag="VKQ52_09160"
     CDS             2550..2885
                     /locus_tag="VKQ52_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87398.1"
                     /translation="MSPLTLRTDFGCTEAVIDDDCGLKRFYEVANILSDDLDIKFTQK
                     TDDFDSLMWDFLYKGHILTLHYNIYTGISIYPRKFREAPRKENDAVVEVAKFLESKLL
                     INTARRYMS"
     gene            2983..>4016
                     /locus_tag="VKQ52_09165"
     CDS             2983..>4016
                     /locus_tag="VKQ52_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLZ87399.1"
                     /translation="MTSIRQTPFYTKLAMVLISLIALFYIAVLGKTVLVPLVFGLLFS
                     VMLLPVANFFERRLRLPRSLASILSVLLLVCSLVVLLYVVGAQVSNLADDWPQFKEQV
                     TTSVADLQRWISTKFHIRIKQQMTYVNNATSKLLETGGTVLGDVVVSFSSVLLTMVFI
                     LIDTFFLLYYRRLIIRFLVAVFREENSVIVYDVLAQIQSRIRQYILGLLLEMVIVSVA
                     TCLALWILGIKYAILLGLITGLFNVIPYIGIFTATLLSTLVTFATAGATKLLLVIATI
                     LGIHLIDSNVLLPVVVGAKVRINAFITVLGVIIGEAVWGIPGTFLAIPIIAIAKIVFD
                     RIEPLKPWGYL"
BASE COUNT          838 a          959 c         1213 g         1006 t
ORIGIN      
        1 agttaatttt taggcttttt caatatcagg gcgccagcct cgagcagccg ttcgaggtct
       61 ttgtagcgca acaagaggtc ggctctttct tccgggttgc caaattccgt gttatccgtt
      121 gtgaggcatg tctgtaccat ggcccagagg atataacggc attcgtgcag gcgatagtcg
      181 ctaaaaaaac gttcgaacac gccgatgggg tcctgcattt cctcttcccc cagcagcaag
      241 acctggtcga acaattcttt tctttccaaa aagacggaca taagattgat ttttaaggtt
      301 aacacttggc tgcatccggg ggggccggcg tgagggatgt cgaacggcgg atgctccact
      361 tatccgctat agtatggtga ggatcggccc tgggggggcg agagattcag aggggtaaaa
      421 ttaatgaaaa aaatctatcg aggataaaat tgtggtgcag gcttttcgga ttgccgcttc
      481 ggtgatggat agtggagggg cgatgcgcag gcaatgtggt gcgaagaggt accagtcggt
      541 gagcaggccg tttgcgatgc aggtatcgat gatgcgtttg ttctgttcga aattatcaaa
      601 ttctacggcg atgaggaggc cgctgctgcg taccgccttt attgccgggt gaacgagcag
      661 gcgccggaac agtgcttcct tttgagccac tgcttctatc cagcctgatc cgagcagcac
      721 ctgcatggag gcgaggcctg ccgcgcagca gagcgggtga ccgccgaatg tcgtgatatg
      781 gccgaggacg gggtcgccgg tgagggtcca catccgttcg cgaccggcga tgaatgcgcc
      841 catcggcagt ccgccgccga gtgcttttcc gaggaggagg atgtccggga taatgttgta
      901 ccgctgaaag gcccagaggg ttccggtccg gccgaagccg gcctggatct catcgaagac
      961 gagaagcgtg ccggtctcat cacagcgttg gcgcagctgc cgcagccagg tctcatcggg
     1021 ggcgaagacc cctctttccg cctgcacggt ctcggcgatg acgcaggcgg tgtgggtggt
     1081 gatggcgtcg agtgccgcgg gagtattgta tggtacatgc cggatgccgg gcagcagggg
     1141 gcggaaggca tttcgccagt attcgtcgcc gatgatgctg agggcgccct gggtggagcc
     1201 atggtaggat tggtggaagg caacgatctc cggccggccc gtgatgcgtt ttgccagttt
     1261 catggcacct tcggttgctt cggcgccgct gttcgtgaag taaacggagt ccagggaagg
     1321 gggaagatgg gcggccagga gggaggcata ttccacctgt ggagactgga tcagttcgcc
     1381 gtagaccagg aggtgcaggt agcgatcggc ctggtctttt atcgcttcca ccacccgggg
     1441 atgacggtga ccggtattac agacgctgat gccgccgatg aggtccaggt attccttgcc
     1501 gttcttatcg tagaggtaga ggccttcggc ttttacgatc tccaggccca gcgggtttgg
     1561 cgatgtttgt gcgacatggc ggtggaagag acggtgatta tcctggtaat ggctcatgga
     1621 agcgcaaagt tcgtaaattt gaacattaac taacaatcaa acaccacgat catggctacc
     1681 atactaccgg ttgaaggcaa atctccccgc tttggaaaga actgttttgt cgcgcccaat
     1741 gccacggtgg tgggggatgt cgagatgggg gatgaatgca gtatctggtt caacgcggtc
     1801 gtgaggggtg atgtgaactg tatccggatc ggtaataagg tgaatatcca ggatggggtg
     1861 atcctgcatg gaacgtataa gaaggccagt gttaccatcg aggattgtgt ttcgatcggg
     1921 cacaatgcga tcatccatgg atgtacgatc cgggagcggg tgttgatcgg gatgggtgcg
     1981 atcatcatgg acaatgcgga gatcgggaag aactcgatca tcgccgccgg cgcggttgtc
     2041 ctggagggga cgaaggtgga gtcggggacg atctatgggg gtatcccggc gcgcaagatc
     2101 aaggatgttc cggccgacct gatctccggg cagatcgatc ggattgccaa tcattatctt
     2161 atctatgcgg gctggttcaa ggatgccaat gacgccccgc agccttagac ctacccattt
     2221 accatgaaag ggcgggttaa ccgcaaaagg tagggggatg cttgcaaatg caggataata
     2281 ttcgtaggtt taagtacgtt tatatctgta tagatcccat gaaactaccc aagaccataa
     2341 ttctccttta tttcttttcg ttccttgtta caagtctgtc ttcctgtagt cttttcggac
     2401 caaagtatgg ctgtccgagt gatggcaaga atgtgggcgc ggagcggatc ctgagtggag
     2461 agaaagttcc aagatcgccg aagttcaaag catgaccgat aaaccccgta actcaatacc
     2521 ctaatataaa acttaaaaat ccctacacta tgagccctct aactctacgc accgatttcg
     2581 gctgtacgga ggccgtgatc gacgacgact gtggattgaa gcgtttctat gaagtcgcta
     2641 acatcctttc cgacgacctg gatattaaat tcacccaaaa gaccgatgat tttgattcgc
     2701 tgatgtggga tttcctttac aagggacata tcctgacact gcattacaac atctataccg
     2761 gtatttcgat ctatccccgc aagttccggg aggcgccgcg gaaggagaac gatgcggttg
     2821 tggaagtagc caaattcctc gaaagtaagc tattgatcaa tactgcccgg cggtacatgt
     2881 cctgacagca acgagggagt gtatagctga cgattgtttg ctgaatgttg tagcaacccg
     2941 tgttaacgca cgggttttgc ttttttgcct atattgcgat acatgacatc catccgacaa
     3001 accccctttt ataccaaact agccatggtg ctgatcagcc tgatcgccct gttctatatc
     3061 gcggtcctgg ggaagaccgt tcttgtgccg cttgtctttg gcctgttgtt ctccgtgatg
     3121 ttgttaccgg tggcgaattt tttcgagcgg cggctccggc ttccgcggag cctggcttcc
     3181 atattgtccg tgttgctgct tgtctgttcc cttgtcgtgc ttttgtatgt cgtgggggcg
     3241 caggtcagca acctggccga tgactggccg cagttcaagg agcaggtgac gaccagtgtc
     3301 gccgatctgc agcgatggat ctcgaccaaa ttccatatcc ggatcaagca gcagatgacc
     3361 tatgtgaaca atgcgacgtc gaagttgctg gagacgggcg gtacggtcct tggcgatgtc
     3421 gtggtctctt tctcgtcggt tttgctgacg atggtgttca tcctgatcga tacttttttc
     3481 ctgttgtact accggcggct gatcatccgc tttctcgtgg ccgttttcag ggaggagaat
     3541 tcggtgatcg tgtatgatgt gctggcgcag atccagagcc ggatacggca atatatcctg
     3601 gggttgctgc tggagatggt gatcgtttcc gtggcgacct gtcttgcgtt gtggatcctt
     3661 gggatcaagt atgcgatcct gctggggctt atcaccgggc ttttcaatgt cattccttat
     3721 atcggcattt ttacggcgac cctgcttagt acgctggtga cctttgcgac ggcgggggca
     3781 accaagctct tattagtgat cgccacgatc ctggggatcc atctcatcga ctccaatgtg
     3841 ttgttgccgg tggtcgtggg agcgaaggtg cggatcaatg cttttatcac cgtgcttggg
     3901 gtgatcatcg gggaagcggt ctgggggatt cccggcactt tcctggcgat cccgatcatt
     3961 gccattgcca agatcgtgtt cgaccggatc gagccgctta agccctgggg atacct
//
LOCUS       DATGFG010000496         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1408664, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000496 DATGFG010000000
VERSION     DATGFG010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408664"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VKQ52_09170"
     CDS             <1..241
                     /locus_tag="VKQ52_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87400.1"
                     /translation="LFPGTWLGRIPFMMMAFYLFVVCVLLQVAFSLLYPGDQPAGTTP
                     LYWTSPFEPLKRPGNYKLISLVLFVVVGILFFIFR"
     gene            260..1075
                     /locus_tag="VKQ52_09175"
     CDS             260..1075
                     /locus_tag="VKQ52_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ87401.1"
                     /translation="MKILNEKVIFLTGGSRGIGLECAKKYREAGAYVVIVTNDAVSLK
                     EAMALLAETTGQPGEATGQLGEAVSGLLCDVSRPLEVEQAIRQTLQRYGRIDVIHNNA
                     GIAHPSKPLEETDEPEWDRLFAINLKSILFTTQYGLEALKSSAGSILNTSSLVGEIGQ
                     ENHAAYAATKGAINALTKSMALDYARYKIRVNAVAPAGVWTPLLREWAHAQENEGTIA
                     EYLDGIHALGYCPEGDVVADACVFLASDQARFITGAILPVSGGAELGYRRIMK"
     gene            1072..2370
                     /locus_tag="VKQ52_09180"
     CDS             1072..2370
                     /locus_tag="VKQ52_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLZ87402.1"
                     /translation="MKTKTIIFGLLLLTLPYSKTVAQSDSLSPHSGSLSPHSDSPQLP
                     YSDYFTPRDGLHGFYTAVTKQKKATVAFLGGSITFNPGWREKVCTYLTARFPETDFHF
                     IAAGIPSLGSLPHAFRVQQDVLDSGRIDLLFLETAVNDRVNGTDSLTQVRCLEGILRH
                     VRKANPGGDIIMMAFADSDKIADYDRGIVPVEIANQDLVAGHYGLPSINLGKEVHDKI
                     RNKEFDWKLDFRDLHPSPFGQELYFQNIKCLLDTCGKWYPDSSANGSSPFSSANASSP
                     ISSANGSSPPPLDNANLDNGRYAPVSQARHNNAWILHRDWQPADTAGTRPGFVHVPVL
                     SGERPGAKLTLPFEGTAVGIAVVSGPDAGIVEYAIDNGPWRSVNLFTKWSNRLHLPWF
                     ILFGSGLPPGSHVLQLRIGQEKDPNSRGHACRIVHFLVNR"
     gene            2489..3085
                     /locus_tag="VKQ52_09185"
     CDS             2489..3085
                     /locus_tag="VKQ52_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ87403.1"
                     /translation="MTSNGHEKIIDYVSKFVGLTDEENNAFAACFKELRVVRRQFIVQ
                     PGFTARSRYFVIKGALRAYVVGDEGQDHTIQLAIDEWWISDYNSYIFQQPASMFVVAL
                     EDSVLLQINFEDEKLLKASNYKFETFFRILAERSTAFMQRRLIVNLTMSAEERYNSFM
                     EKYPLMAERIPQYAIASYLGMTTEFLSKIRNKKVKRNH"
     gene            3182..>3838
                     /locus_tag="VKQ52_09190"
     CDS             3182..>3838
                     /locus_tag="VKQ52_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ87404.1"
                     /translation="MQQLQHKVAVVTGGSSGIGLGIAKRFAAEGSKVVITGRNKTSLD
                     KAVAEIGHGTLGIQGDVSHLHDLDRIFRTVDEKLGKIDILVANAGSYIVGPLAEFTEE
                     MFDQLSDINFKGTFFTVQRALPALKDGASVVLVSSTVNGKGVPNHAAYSATKAAIRSL
                     ARSFSAELLARKIRVNTLTPGPVDTPIFSTVAGSPEDAKAMMEAMANFTPLKRVGTTE
                     E"
BASE COUNT          915 a         1101 c         1008 g          814 t
ORIGIN      
        1 gctctttccg ggcacctggc tgggccgcat ccccttcatg atgatggcct tttatctttt
       61 cgtcgtatgc gtgctgctgc aggtggcctt ctccctgtta taccccggtg atcaacccgc
      121 cgggaccaca ccgctatatt ggacctcgcc cttcgagccg ctgaaacggc ccggaaatta
      181 taaactgata tccttggtac tattcgttgt ggtgggaata ttattcttta tttttcgcta
      241 actgtcccat aaaagacgca tgaaaatatt gaacgaaaaa gttattttcc tgacaggcgg
      301 atcccgcggc atcggtctgg aatgcgcaaa aaaataccgg gaagccggcg cctatgtagt
      361 catcgtgacc aatgacgcgg tatccctgaa agaggccatg gcgctccttg ccgaaaccac
      421 aggacaaccg ggcgaggcca caggacagct gggcgaagcc gtctccggcc ttctctgcga
      481 cgtatcccgg cccctggaag tggagcaggc catccgccag acgctgcaac gatatggaag
      541 gatcgacgtt atccacaaca atgcgggcat cgcccatccg tcaaagcccc tggaggagac
      601 ggatgagccg gaatgggaca ggctttttgc catcaacctg aaaagtattt tatttacaac
      661 gcaatacggg ctggaagcct taaagtcatc cgcgggatcc atcctcaaca ccagtagcct
      721 ggtgggggag ataggacagg agaaccatgc cgcctatgcc gctaccaaag gcgcgatcaa
      781 tgcactcacg aagtccatgg cgctggatta tgcccgatat aagatccggg tcaacgccgt
      841 ggcgccggca ggagtgtgga cgcccttgct ccgggaatgg gcccacgccc aggaaaatga
      901 agggacgatc gcggagtacc tcgatggcat ccatgcgctc ggctattgcc ccgaaggaga
      961 tgtggtagcg gacgcctgcg tgttcctcgc gtcggatcag gcgcgattca tcaccggcgc
     1021 catcctgccg gtcagcgggg gggcagaact gggctaccgg cggatcatga aatgaaaacg
     1081 aaaactatta tattcggcct gctcctcctg accttgcctt acagcaaaac ggtcgcccaa
     1141 tcggattccc tgtcacctca ctcgggctcc ctgtcccccc actcggattc cccccaactg
     1201 ccgtattccg attattttac cccacgggac ggcctgcacg ggttctacac ggccgttaca
     1261 aaacagaaaa aggccacggt cgccttcctg ggcggctcca tcacctttaa tcccggctgg
     1321 cgcgagaagg tctgcactta tctgacggca cggttcccgg agacggactt tcacttcatc
     1381 gcggccggta tcccttcact ggggagcctt ccgcatgctt tccgggtgca acaggatgtc
     1441 ctggactccg gcaggatcga cctgctgttc ctcgaaaccg ctgtcaacga cagggtcaac
     1501 gggacggaca gtctcaccca ggtgcgttgc ctcgaaggga tcctgcggca cgtaagaaag
     1561 gccaatcccg gcggagacat tatcatgatg gcatttgccg actcggataa gatagcggat
     1621 tatgatcgcg gcatcgtccc ggtggagata gccaaccagg atctggtggc cggtcattac
     1681 ggactgccct ccatcaatct cgggaaagag gtgcacgata agataagaaa taaggaattc
     1741 gactggaaat tggacttcag ggatctgcac ccttccccat tcggccagga attgtatttt
     1801 caaaatataa aatgcctgct ggacacctgc ggtaagtggt accccgactc ctccgcaaat
     1861 ggatcgtctc ccttctcttc cgcaaacgca tcatccccca tctcctccgc gaatggatca
     1921 tcccccccac cattggataa cgccaacctg gacaacggcc gctacgcacc tgtttcgcaa
     1981 gcccgccaca acaacgcatg gatccttcac cgcgattggc agcctgcgga cactgccggc
     2041 acccgcccgg ggttcgtgca tgtgccggta ctgagtggag aacggccggg cgcaaaattg
     2101 acccttccct tcgaagggac agcggtcgga atagccgttg tatccggccc ggatgccgga
     2161 atagtagaat atgccatcga taacggtccg tggcgctccg taaacctgtt tacaaaatgg
     2221 agcaatcggc tgcacctgcc ctggttcatc ctgttcggaa gcggacttcc ccccggcagc
     2281 catgtcttgc aactgcgcat cggccaggag aaggatccaa acagcagggg ccatgcctgc
     2341 cgcatcgtac atttcctcgt taatcggtaa agccttcccc ccacggcccc accgccccac
     2401 cgcgccgtcc ccccacggcc ccccgacgcc gtccccgctt attgaaaaac taacctatcc
     2461 tgtgctatat ttgcggtata tagccaccat gacatccaac ggccacgaaa agataatcga
     2521 ctacgtctcg aaatttgtcg ggctaaccga tgaggagaac aacgcatttg ccgcctgctt
     2581 caaagagctc agggtcgtca ggagacaatt catcgtgcag cccggattta cagcaaggag
     2641 caggtatttt gttataaaag gggccctgag ggcctatgtg gttggggacg aagggcagga
     2701 ccatactatt caattggcta ttgacgaatg gtggatctcg gattacaaca gctatatctt
     2761 tcagcaaccg gcgagtatgt tcgtcgtcgc actggaggac agtgtcctgc tgcagatcaa
     2821 ctttgaagat gaaaagctgc tgaaagcatc gaactataag tttgaaacct tctttagaat
     2881 actggccgag cgttccactg ccttcatgca aagaaggctt atcgtcaacc tcacgatgtc
     2941 tgccgaagag cgatacaaca gcttcatgga aaaatatccg ctgatggccg aacggatacc
     3001 ccaatatgcc atcgcctcct acctgggaat gacaaccgag ttcctttcaa agatccgcaa
     3061 taaaaaagta aagcgcaacc attagttgaa ctacttcaac tgtttttcct aacctccttc
     3121 atttttaaaa gccgtgggct gtaagacctt tgcggagcaa caggtaaaaa taaataacta
     3181 tatgcaacaa ttacaacaca aagttgctgt tgtcacaggt ggcagcagcg ggataggtct
     3241 gggaattgcc aaacgtttcg ccgccgaagg ctcgaaagtg gtgattacag gtaggaataa
     3301 gacatccctg gataaggcgg tggcggagat cggtcatggt acgctgggca ttcagggcga
     3361 tgtgtcccac ctgcacgatc tggataggat tttcagaacc gtcgatgaaa agctgggcaa
     3421 gatcgatatc ctcgtcgcca acgcgggttc ttacatcgtg gggccgctgg ccgaattcac
     3481 cgaggagatg ttcgaccagt tgagcgacat caatttcaag ggaacattct tcacagtcca
     3541 aagggctttg cccgcgctga aagatggcgc ctccgtggtg ctggtgtctt ctaccgtcaa
     3601 tggcaaaggc gttcctaacc atgcggcgta ttccgcaacc aaggcagcca tccgttcgct
     3661 ggcccgtagc ttttcggcgg agttgctggc ccggaagatt cgcgtcaata cccttacccc
     3721 cgggcccgtc gatacaccga tcttcagcac cgtagccgga agtccggaag atgcaaaggc
     3781 catgatggaa gctatggcca attttacacc gctgaaaagg gtcggtacga cggaagag
//
LOCUS       DATGFG010000497         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_136193, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000497 DATGFG010000000
VERSION     DATGFG010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136193"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VKQ52_09195"
     CDS             <1..1117
                     /locus_tag="VKQ52_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HLZ87405.1"
                     /translation="RFSLSWARILPEGIGRVNKEGIAFYHRVIDECLKLGLTPYVTLY
                     HWDMPMTLEKEGGWTSHLMLKWFTRFVTLCAEEYGDKVKNWIVLNEPFGFTSLGYMLG
                     RHAPGRVGLSNFLPAIHHAALAQAEGGRILRQLVPGAHIGTTFSCSEVLPFTNNPEDI
                     QAAKRMDILMNRLFIEPALGRGYPREDFKFLEKLELHNRSWKYTERMTFNFDFIGIQN
                     YFPIVVRYNPLIPIVQASEVKAAARKVPYTAMGWEINPDSFYRILKRFWLYGGVKEII
                     VSESGAAFRDELVDGAVDDQARVDYYQQYLLALLRAKKEGVNIKGFFAWTLMDNFEWA
                     EGYRARFGLIHVDFDSQLRTIKNSGYWFRDFLLGNKR"
     gene            1422..2636
                     /locus_tag="VKQ52_09200"
     CDS             1422..2636
                     /locus_tag="VKQ52_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLZ87406.1"
                     /translation="MHEKKLLYKGKIIRKLYFSSALSCSDLSTQIKTSLPLTTKMVNE
                     LIRDNDIIETGYAPSTGGRRPQMYSLRPDRMYTVAVAMDQLFTRIVIMDMQRRYVSDP
                     EKIELQLTKNPLALETLATKLNEHIHKSGVSREKIVGIGIGMPGFVDARKGLNYSFLG
                     NGETSITQYISEKVNVPTYVENDSSLIALAELSFGAARHKKSAMVINLGWGIGLGLIL
                     NGELFRGYNGFAGEFSHLPLFTNDKLCSCGKMGCLETEASLMVVVEKAKEGIRNGRVT
                     LLQEKQLTDNLDHASRAVISAAGKGDKFAVELLSEAGYHIGRGVAILIHLLNPEAIVL
                     SGRGSFAGKIWQISLQQALNEHCIPRLSANTTIEVSELGWEAELIGAAALVMDNYVKG
                     SIKKGTSTVMVR"
BASE COUNT          747 a          866 c          719 g          636 t
ORIGIN      
        1 ccgcttctcc ctgtcatggg cccgcatcct ccccgaaggc ataggccggg tcaacaaaga
       61 aggcattgcc ttctatcatc gcgtcatcga cgaatgcctt aaactgggcc tgacccctta
      121 cgttaccctc tatcactggg acatgcccat gaccctcgaa aaagaagggg gctggacaag
      181 tcacctgatg ctcaaatggt tcacccgctt cgtcaccctc tgcgccgaag aatacggcga
      241 taaggttaag aactggatcg tcctcaacga acccttcggc ttcaccagcc tgggctacat
      301 gctcggccgc catgcgccgg gtagggtagg gctctccaac ttcctccccg ctatccacca
      361 tgcggccctg gcccaggccg aaggcggccg catcctccgg caactcgtcc ccggcgccca
      421 tatcggcacc accttctcct gctccgaggt gctgcccttc accaacaacc ccgaggacat
      481 ccaggcggca aaacgcatgg acatcctcat gaaccgcctc ttcatcgaac ccgctctcgg
      541 ccgcggctac ccgcgcgaag acttcaaatt cctcgagaaa ctcgaactcc acaaccggag
      601 ctggaaatac accgaacgga tgacctttaa cttcgatttc atcgggatcc agaactattt
      661 ccccatcgtc gtccgctata accccttgat ccccatcgtc caggcctccg aggtaaaggc
      721 cgccgcccgc aaggtcccct acaccgccat gggttgggag atcaaccccg acagcttcta
      781 ccgcatcctc aaacggttct ggctatacgg cggggtaaaa gagatcatcg tcagcgaaag
      841 cggcgccgcc ttcagggatg aacttgtcga tggcgccgtc gacgaccagg cccgtgtcga
      901 ctactaccag caatacctgc tcgccctcct ccgggccaaa aaggaagggg tcaatataaa
      961 aggcttcttc gcctggaccc tcatggacaa tttcgaatgg gccgaaggct accgcgcccg
     1021 gtttggcctc atccacgtag actttgattc tcaactccgt accatcaaaa actcggggta
     1081 ctggttccgc gactttctcc tcgggaacaa aaggtagcgg cctctctctc ccactcgagc
     1141 ggcttccccc accacttaag cgccgccgcc ctccccctta accgccctcc ccctccccct
     1201 caaactgccc ccaagagcca tccttctacc caattaagag ctatcttcct actatttttt
     1261 aaaaactccc tagtaaaaac taaatagacc atcgtaaatg gtctattttt ttcgatgtcg
     1321 tatcaacttt cataaaaaat ataatttctt cttaactttt ttataaaagt ttattaactt
     1381 taattttatt actaatcgct cctgtacgct gactgatccc tatgcatgag aaaaagctgt
     1441 tatataaagg aaagattatc aggaaattat atttctcgag cgccttatcc tgttccgatc
     1501 tgagcaccca gatcaagaca agcctgcccc taacgacaaa aatggtaaat gaattgatca
     1561 gggacaacga tatcattgaa acaggttacg ctccttccac cggcggacgg cgtccccaaa
     1621 tgtattccct gcggcccgac aggatgtata ccgttgccgt cgccatggac caattgttca
     1681 cccgcatcgt catcatggac atgcaacgcc gttatgtcag cgatccggag aagatcgaac
     1741 tccaactgac caaaaatccc ctggcgctcg aaacgcttgc cacaaaactg aatgagcaca
     1801 tccataaatc cggggtaagc agggaaaaga tagtcggcat cggtatcggt atgccgggat
     1861 tcgtagacgc aaggaaaggc cttaactatt ccttcctggg taacggggag acgagcatta
     1921 cacaatatat atcggaaaag gtcaatgtgc ccacctacgt cgagaacgat tccagcctca
     1981 tcgcactcgc cgagctcagc tttggggcgg cgcggcataa gaaaagcgcg atggtcatca
     2041 acctgggctg gggcatcggc ctcggcctga tcctcaacgg cgagttgttc cggggataca
     2101 atggtttcgc cggcgaattc agccatctgc cgctgttcac caatgataaa ctatgtagct
     2161 gcgggaagat gggctgtctc gaaacagagg cctccctgat ggtcgtagtg gagaaagcca
     2221 aggaaggcat ccgcaacggg cgcgttaccc tgttgcagga aaaacagctg acggacaacc
     2281 tcgaccatgc atcgagggca gtgatctccg cggcaggcaa aggggacaaa ttcgccgtgg
     2341 aactgctgtc cgaagccggc taccatatcg gcagaggtgt cgccatcctt attcacctgc
     2401 tgaacccgga agcgatagtg ctcagcggaa gagggtcatt cgcgggaaag atctggcaga
     2461 tatccctgca gcaggccttg aacgagcatt gtatacccag gctttcggcc aatacgacca
     2521 tcgaggtgtc tgagctgggg tgggaggcgg aactcatcgg ggcggccgcg ttggtaatgg
     2581 acaattatgt aaaaggatcg ataaagaaag gaacgagtac agtcatggtg cgctgaggat
     2641 ggaacagcat gggaaaaatg attgaacggc cataggaaag ataatcaaaa cttcattttc
     2701 ttaaatcaaa actacaacgt taatgagaaa gttaaaggct catttgctcc tattcatgct
     2761 atgctgcatg gggagcgcag cactacttgg ccaggaccgg atatcagtag ccggtaacgt
     2821 cagggatagt gttggaaatc ccgtctccag tattactgtt atggtcaaag gcgccaaatc
     2881 gggcacctcg actgacgccc ttggcaattt caagatcagc gtgccaaggg gaggacacct
     2941 cgtgttttcg ggtatcgggt acctgtcc
//
LOCUS       DATGFG010000498         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_479149, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000498 DATGFG010000000
VERSION     DATGFG010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479149"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1106)
                     /locus_tag="VKQ52_09205"
     CDS             complement(84..1106)
                     /locus_tag="VKQ52_09205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLZ87407.1"
                     /translation="MKKIGFLSFGHWSNHPGYKTRTAGDTLLQSIDLAVAAEEIGLDG
                     AYFRVHHFAAQLASPFPLLSAVGAKTSKIEIGTAVIDMRYENPLYMVEDAGAADLISG
                     GRLQLGISRGSPEQVIDGWRYFGYQPAEGETDADMGRNKALQFLDKLKGVGFAQPNPS
                     PMFPNPPGLLRLEPHSGGLRERIWWGAASNATAVWAAENGMHLQSSTLKFDESGKPFH
                     IQQAEQIRLYKEAWKKAGHQRKPRVSVSRSIFALMTDQDRHFFGQEANRVDKIGIIEN
                     DTRAIFGRTYAGEPDQLIRELAQDEAIQEADTLLLTIPNTLGVDYNVHVLSSILKHVA
                     PGLGWR"
     gene            complement(1218..2456)
                     /locus_tag="VKQ52_09210"
     CDS             complement(1218..2456)
                     /locus_tag="VKQ52_09210"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HLZ87408.1"
                     /translation="MKRLLPVHPLHPIVLLRRIASHHRNPPFLRIHRLLPIILLLLLA
                     TSNPLRAQQPAFIHPGILHSAASLAHIYSVAQNRTMPEYGSYELLRDHPLSSAQYKMK
                     GPFAVISRDGAYAYTKSSFEADFSAAYLNALMWAATKDSAHAKRSLAILEAYADSLTT
                     IPSTNDAPLLAGLEGMKIINAMEILRYTYKEIPAAAMNKINRMIRSVFLPVCEKFYDT
                     PAYTNGNWGPIVTKTYLSAAIWFDSRTMYKKAIDFYYNANDNGTIRNYISGATGQIQE
                     SGRDQGHSQLGIGALATVCEIAWNQGDDLYSALDNRLLKGFEYVAKYNLGHDDVPFTT
                     WKDITGKYSNWTTISPTSRGRFIPVYEMVYNHYVRRKGLSMPYTAEVLKKIRPEGYDR
                     DQPAFGTLLFYGAGSPTPGH"
     gene            complement(2453..3352)
                     /locus_tag="VKQ52_09215"
     CDS             complement(2453..3352)
                     /locus_tag="VKQ52_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLZ87409.1"
                     /translation="MPLAPENKTPHPSNLIAMLVDHVSDPFFAGFARRIDRRARTLGY
                     KLVLGSTENDISFMPKLISVFNNMNVAGYIIAPPPGVDPDLKKLLQKGKPVVLFDRYP
                     TDPGPYTVLSDNFKGAYEAVGHFVRNGHRHIALVTVGSNQKRITDRRKGYSAAIDNHR
                     LPSYITELPASASADQVKTAIRTLLQANKAIDAVLFADYSLTFTGLAALRQQKKRIPD
                     HIAVIGFDDNENFQLFAPALTAVVQPLDEMVENILQCIAGHSGEKSARRRPTPIVLPT
                     RMVIRSSTIRTPSTLHLSTTLHR"
BASE COUNT          716 a          779 c         1124 g          847 t
ORIGIN      
        1 cattgtactg tcatgctgta ggcgcccatg gatctaagtt gttgccagat ttcgtccgaa
       61 aatcatgtta gccagactaa agtttaacgc cagcctaatc cgggtgcaac atgcttcaaa
      121 atagaagaca gcacgtgtac attgtagtcg actcccaacg tattgggtat cgtcaacagg
      181 agcgtatccg cttcctggat ggcctcgtcc tgagccaact ccctgatgag ttgatccggt
      241 tccccggcat aggttcttcc gaaaatggcg cgtgtatcat tttcaataat gccgatctta
      301 tcgactctat tggcttcctg cccaaagaaa tgtctgtcct gatcagtcat caacgcgaaa
      361 atggaccggc tcaccgaaac ccttggcttg cgctgatgtc ctgccttttt ccatgcttct
      421 ttgtacaacc tgatctgttc tgcctgttgt atatggaaag gcttgccgct ttcgtcgaat
      481 ttcagggtgg aactttgcag gtgcatcccg ttttcagccg cccaaacagc tgtagcatta
      541 gaggctgctc cccaccaaat acgttcccgc aatcccccgg agtggggttc caatcgtaac
      601 aggcccggtg gattagggaa catgggggac gggttgggtt gggcaaatcc gacacctttg
      661 agtttatcca ggaattgtaa cgctttgttg cgtcccatat cggcgtctgt ttctccttca
      721 gccggttgat acccgaaata acgccatcca tcgatcactt gttccggcga acctctactg
      781 attcccaatt gcaatcgccc gcccgagatc aagtcagcgg ctccggcatc ttccaccata
      841 tagaggggat tttcataccg catgtcaata acggctgtcc cgatctctat tttgcttgtt
      901 ttggcgccga cggccgaaag caaaggaaat ggagatgcta actgagccgc aaaatgatgt
      961 acccgaaaat atgcaccatc taaaccgatc tcttcggcag caacggccaa atcaatggat
     1021 tgaagcaagg tgtcgcccgc cgtgcgggtt ttatagcctg gatgattgga ccaatgtccg
     1081 aatgataaaa atcctatttt cttcatgcgt tccttttcga ttgtactata gcctttgcaa
     1141 agatagccat aatatcattg cccggcaccg aaggagaacc cggcaaagtt agaggtcttt
     1201 gatgcggtgg gcggaggtta gtggccggga gttggggaac cggcgccata gaagaggagg
     1261 gtgccgaagg cgggctggtc gcggtcgtag ccttcgggcc ggatcttttt gaggacctcg
     1321 gcggtgtagg gcatggagag gcctttgcgt cggacgtaat ggttatagac catttcgtag
     1381 acagggatga aacggccgcg gctggtgggt gagatagttg tccagttgga gtatttgccg
     1441 gtgatgtcct tccaggtggt gaagggaacg tcatcgtggc cgagattgta tttcgcgacg
     1501 tattcaaatc ctttcagcag ccggttgtcg agggcggagt agagatcgtc gccctgattc
     1561 cacgcgatct cacataccgt tgcgagggcg ccgatgccga gttgcgaatg tccctgatcg
     1621 cggccactct cctggatctg gcccgtggcg ccgctgatat agttgcggat ggtgccgttg
     1681 tcgttggcgt tataataaaa atcaattgct ttcttgtaca tggtccgcga gtcgaaccag
     1741 atagcggcgg agaggtaggt ctttgttacg atcgggcccc agttgccatt cgtataggcg
     1801 ggggtgtcgt agaatttttc gcagacgggc aggaagacgg agcggatcat ccggttgatc
     1861 ttgttcatgg cggcggcggg aatctcctta tacgtatagc gaagtatctc catggcgttg
     1921 atgatcttca tgccttcgag cccggcgagg aggggggcat catttgtcga cgggatcgtg
     1981 gtcaggctgt ccgcataggc ttcgaggatg gccagggacc gttttgcgtg tgccgagtct
     2041 tttgtcgccg cccacatgag ggcgttgaga taagcggcgc tgaagtcggc ctcgaaggag
     2101 gattttgtgt atgcgtaggc gccgtcgcgg gagatgacgg caaaggggcc cttcatctta
     2161 tattgggcgc tggacagcgg atgatcgcgg agcaattcgt aggagccata ttcgggcatc
     2221 gtccggtttt gcgcgacgga atagatatgg gccagggaag cggcggaatg caggataccg
     2281 gggtggatga aggccggttg ttgggcgcgg agagggttcg aggttgccag caggaggagc
     2341 aggatgatcg ggaggagtcg atggatccgg agaaatgggg ggttccggtg gtgtgaggcg
     2401 atccggcgaa gtaagacgat cgggtgaagt gggtggaccg ggaggagtcg tttcatcggt
     2461 gtaaggtggt tgagagatgc agggtggagg gtgttcggat ggtggaggag cggatgacca
     2521 tgcgggtggg caggacgatg ggggtcggcc ggcgcctggc gcttttttcg ccggagtggc
     2581 cggcaatgca ttggaggata ttctctacca tttcatcgag aggttggacg acggcggtga
     2641 gggccggtgc gaagagctgg aagttctcat tatcgtcgaa accgatgacg gcgatgtgat
     2701 cgggtatccg ctttttttgc tggcgcaggg cggcgaggcc ggtaaaggtg aggctgtagt
     2761 ctgcgaacag gacggcgtcg atggccttgt tggcctggag cagggtgcgg atggcggttt
     2821 tgacctgatc ggcggaggcg gatgcgggca attcagtaat ataggatggg aggcggtgat
     2881 tatcgatggc ggcggagtat cctttccggc ggtcggtaat gcgcttttga ttggagccga
     2941 ctgtgacgag ggcgatatgg cggtggccgt tgcggacgaa atgcccgacg gcctcatagg
     3001 ctcctttgaa gttgtcggag aggacggtgt acggaccggg gtcggtggga tagcggtcga
     3061 agaggacgac cggttttcct ttttgcagga gctttttcag atcggggtcg actcccgggg
     3121 gcggtgcgat gatatagcct gccacattca tattattgaa aacggatata agttttggca
     3181 taaagctaat atcattttcc gtgctgccca ggactaattt gtagccgagg gtgcgggcgc
     3241 ggcggtcgat gcgtcgggcg aagccggcga aaaaggggtc cgagacgtgg tcgaccagca
     3301 tggcgatgag gttgctggga tggggggtct tgttttctgg tgcgagcggc ataacaatcg
     3361 ggttttaatc agagaacctg tttataaagc taggcggcaa atccgctttt ccaaaggggg
     3421 ctttctcata aaaagagggc aaattggtta tttctaaggt ttgctc
//
LOCUS       DATGFG010000499         5070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1143159, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000499 DATGFG010000000
VERSION     DATGFG010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143159"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VKQ52_09220"
     CDS             <1..1378
                     /locus_tag="VKQ52_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87410.1"
                     /translation="REIGQSFADLGERKQIRFTFVTKADRLCVLFDRDKIERILFNLL
                     SNAFKFTPESGSIQLSLEAGAPCPDGRQPVTIRVTDSGIGIPRDQRERIFERFFQHAT
                     PGVILNQGTGIGLSITREFVQLHGGTIHVESEEGYGSVFTVILPLAPAGIDVIAVEKG
                     PAGELNGVDKEGGGERPDVEEPADAGRPAAGSIKRRRNAPGTGSVRPLILLVEDNEDF
                     RFYLKDNLRREYEVLEAANGKEGWQQALAHPELIVSDISMPYMDGIELTKKLKADKRT
                     SHIPVILLTALTEEAQQLKGLGTGANDYITKPFTFELLNARIKGLLEWNNKLRTAYTK
                     QVKVLTPERDGESGNEKLMKRIVGCLEEHFHGPELSVEFLSRQLGMSRSSLYGKLLEI
                     TGETPVEFIRSFRLEKAAALLEKSDLTIAEIAYEVGFTKPNYFTQAFKTKYKILPSDY
                     PGRQEKKS"
     gene            1755..4895
                     /locus_tag="VKQ52_09225"
     CDS             1755..4895
                     /locus_tag="VKQ52_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ87411.1"
                     /translation="MKRCLPKGNTCYFPERRSRRCNAFSLSFFFLVCIFSSLAGYGQQ
                     RVTATGKVIAGDSAIGGATVQVKGHRGGVQTAADGRFSIDAPKGATLVITFVGFAPEE
                     VKASSEFTVVQLKQAVQQGSEVIVVGYGTQKKATLTGSISQVAGSEIAVSPSPNVGAS
                     LAGKLPGLIVNQTNGEPGKDDPNLLIRGMATIPGPPGSPVNSGPLIIIDGVERSGLTR
                     LDPDDIDAISVLKDASAAIYGARAANGVILVTTKTGHRGKTAFDFEYNYGIQNVTKIP
                     KMLDAATFAQVYNEGLFYREGRPAVFTPFYADSVIQKFRDGSDPVLYPNTDWVKAVLK
                     SSHVQRIGIQASGGSDAVRYLLSFGSTTQDGDFRHVPDNYGQYNMRLKLDADIAPNLT
                     VGANISGIFSDATYSSVTGQPSIGQPGGTSVNFVNILQSNPTLAAVYPNGDIAPGRLN
                     QNPLLLDQEGFDKVSNGSIYSTFTGTYRAPFIKGLRVDISYNYDFANQFEKLWQIPAY
                     YYSYNVNTKNYDHLLQNTVIQDNDIYNKWTTQLTNARLTYDRIFAGKHHVTALAGVEQ
                     QYNTNSFTNSGRKNFVSTAVPQLNVGSSAAADQSVSGSASAGAYNNYFGRLNYDFASK
                     YLLEFSFRDNGSQIFPVGKRYGFFPGGSAGWVLSEEKFMHNIPAITYLKLRASHGELG
                     NDRVYAYQYLQSYSFGSNYVFGSGDVPGIYPNTVPNPNITWEVSKKTDFGLVATLWNG
                     LLGIDATYFLEHRSRILVQPNLSVSNVYGYPSLPSENIGRVDNHGYELVLTHRQSFKD
                     FSYSLSANFSYARSKIIYMDEVPQAEPWQNATGHPIGSALYYKTVGIFKDQADLTRNA
                     NEAGSGSQVGDQKVANLSGGTQITANDQFRFDYSPIPMETFGFTSVFRYKSWDLNLFF
                     YGQSKVYNYDAIYANLGSADFSNAMVARATNRWSVSNPNGSLPRSGEWTPGGTTLYLK
                     DATFIRLKTAEIGYNLPHNLLARTKTFTDFRVYVSGFNVLTWAKAIKYADPEINGNST
                     AYPQQRIINFGCTARF"
BASE COUNT         1213 a         1272 c         1410 g         1175 t
ORIGIN      
        1 ccgggagata ggtcagtctt ttgcggatct cggagagcgg aaacagatcc gtttcacctt
       61 tgtaacaaag gcggacaggc tttgcgtgct tttcgatcgg gacaagatcg agcggatact
      121 tttcaacctg ttgtcgaacg cttttaagtt cactccggaa agcggcagca ttcagttgtc
      181 gctggaagca ggcgcgccct gcccggatgg ccgtcagccg gtgacgatcc gcgttacgga
      241 ttccgggatc ggcattccgc gggatcaacg tgaaaggatc ttcgaacggt tctttcagca
      301 tgcgacgccg ggggtgatcc ttaaccaggg cacgggcatc ggtttgtcga tcaccaggga
      361 atttgtgcaa ctgcatggag gcaccattca tgtggagagt gaagagggtt atgggagcgt
      421 tttcaccgtg atcctgccgc tggcgccggc cggcatcgac gttattgcgg tggagaaagg
      481 gccggcgggg gagctgaacg gtgtggacaa agagggtggt ggtgagagac cggatgtaga
      541 agagcctgct gacgccggaa ggccggccgc cgggagtata aaacggcgga gaaacgcacc
      601 gggtaccggg agcgtaagac cgctgatcct gttggtggag gataatgagg atttccggtt
      661 ttacctgaaa gataacctgc ggagggagta cgaggtactg gaggcggcta acggcaaaga
      721 aggctggcag caggccctgg ctcacccgga gcttatcgtc agtgatatca gcatgccgta
      781 tatggacggg atcgagctga ccaaaaaact gaaggcggac aaacgcacca gccatatccc
      841 cgtgatcctt ttgacggcgc tgaccgaaga ggcgcagcag ttgaaagggc tgggtacggg
      901 cgccaatgac tatattacca agccctttac tttcgaattg ttgaatgcgc ggatcaaagg
      961 tttgctggaa tggaacaata aattgagaac tgcctatacg aaacaggtaa aggtcctcac
     1021 gccggaacgg gatggggagt ccgggaatga gaagctgatg aaaaggatcg tgggctgcct
     1081 ggaagaacat ttccatggtc cggagctctc ggtggaattc ctgagcaggc agctcgggat
     1141 gagccggagt tcgctatatg gtaagttgct ggagatcacg ggagagacac ctgtggagtt
     1201 tattcgctcg ttccggctgg aaaaggcggc tgcccttctc gaaaagagtg atctgaccat
     1261 tgccgagatt gcctacgagg tcggatttac caagcccaat tatttcacac aggctttcaa
     1321 aacaaaatat aagatcctgc cttccgatta cccgggccgg caggaaaaga aaagctagtt
     1381 gttcctaacg tctcagtttt tgattcctcc cgtctcaatc cccgctgatc tatcgtccgg
     1441 gttaccgctt attgtcgccc tgagattata tttgatctgt aacagatgac acttgtatga
     1501 aaggatctgc gatgggtacc ggattgaagg gataatttat tcaatcgatt gaataaaaaa
     1561 gagattagac gggaaagtga acttagactg aatacctaaa ttattaactg tctgtataat
     1621 agatgcaaat aacgaaagaa gatggttttt ttgattttat tcaatcgatt gataattctg
     1681 atttgattgc cgatcgcatt tttaaatgct tttgtcggag aacaaagttc attctcacac
     1741 taaaactctg ctaaatgaaa cgatgtttgc caaaagggaa tacatgctat ttccctgaac
     1801 gccgctcaag gcgttgtaat gctttctcac tttctttctt tttccttgtc tgtatctttt
     1861 cctcgctggc agggtatggc cagcaacggg tcacggcgac gggcaaggtc attgccggcg
     1921 attcggccat cggcggtgcg accgtacagg tgaaggggca caggggcggt gtgcagacgg
     1981 ctgcggacgg aaggttctcc atcgatgctc cgaaaggcgc gaccctggtg atcacctttg
     2041 tcggctttgc accggaggag gtgaaagcca gttccgaatt tacggtcgtt cagttgaaac
     2101 aggccgtgca gcagggcagc gaggtgatcg tggtgggtta tggtactcag aagaaggcca
     2161 cgctgactgg ttctatttcc caggtggcgg ggtccgagat tgccgtaagc ccttccccta
     2221 atgtaggagc gtcactggcg ggcaagcttc cgggcctgat cgtcaaccag accaatggcg
     2281 aacccggtaa ggatgatccg aatttactca ttcgcgggat ggctaccatt cccggcccac
     2341 cgggctcacc ggtgaatagc ggtccgctta ttatcatcga tggggtggag cgtagcgggc
     2401 tgacccggct tgacccggat gatatcgacg ccatttccgt actgaaggat gcttccgccg
     2461 ccatctatgg cgccagggct gccaacggtg tcatcctggt tacgaccaaa acgggacaca
     2521 gaggcaagac ggcattcgat ttcgagtata attatggtat tcagaacgtg accaagatcc
     2581 cgaagatgct ggacgccgct acctttgccc aggtatacaa cgaaggcttg ttttaccgcg
     2641 aaggtcgtcc tgcggtcttc acgccttttt atgcggattc ggtgatccag aagttcaggg
     2701 acggttccga cccggtactc taccctaata ctgactgggt aaaggcagtg ctgaaatcct
     2761 cccacgtgca gaggatcggg atccaggcca gcggcggttc cgatgccgta cggtacctgc
     2821 tgtccttcgg gtctacaacg caggatggcg atttcaggca cgttccggac aattacgggc
     2881 agtataacat gcgcctgaaa ctggacgcgg acattgctcc caacctgacc gttggcgcca
     2941 acatctcggg cattttcagc gacgctacct actcttctgt taccggacag ccttctatcg
     3001 gacagccagg tggcacgagc gttaattttg tcaatatctt acagtcgaac cccaccctgg
     3061 ccgcggtgta tccaaatgga gacatcgcgc ccggacgttt gaaccaaaac ccgctgctgc
     3121 tggatcagga gggttttgac aaggtcagta atggttccat ctattctact tttacgggca
     3181 cttaccgcgc gccgttcatc aaagggttac gggtggatat atcctataac tacgattttg
     3241 cgaaccagtt cgaaaagcta tggcagattc ccgcttacta ttactcctac aacgtaaata
     3301 cgaaaaacta tgaccacctg ttacagaata ccgttatcca ggataacgat atctacaaca
     3361 aatggacgac acagctgacc aatgcccggc tcacctatga ccggatcttc gccggtaagc
     3421 accatgtgac cgcccttgcc ggtgtggaac agcaatacaa tacgaattcc tttaccaatt
     3481 cgggtcgcaa gaattttgtg agcacggccg ttccgcagct caacgtgggc agttcggccg
     3541 cggcggatca gagtgtcagc ggcagcgctt ccgcgggagc ttacaacaac tatttcggcc
     3601 gtctgaacta tgacttcgcg tccaaatacc tgctggaatt ctcgttcagg gacaatggct
     3661 cgcagatctt tcccgtgggt aagcgctacg gcttcttccc gggtggttcg gcaggatggg
     3721 tgctttccga agagaagttc atgcataata tccccgccat cacctacctg aaattacggg
     3781 cttcgcatgg cgaactgggc aacgaccggg tgtatgccta ccagtacctg cagtcttatt
     3841 ctttcgggag caactatgtt ttcgggagcg gcgatgtgcc cgggatctat cccaacacgg
     3901 tgcccaaccc gaatatcacc tgggaggtga gcaagaagac ggacttcggc ctcgtggcta
     3961 cgctgtggaa cggcctgctg gggatcgacg ctacctattt cctcgagcat cgttcgagga
     4021 tcctggtgca gcccaatctt tccgtgagca atgtttatgg ttatcccagc ctgcccagtg
     4081 agaatatcgg ccgggtggat aaccatggct atgagctggt gttgacccac agacagtcct
     4141 tcaaggactt ctcctactcc ctttctgcaa acttcagcta tgcaaggagc aagatcatct
     4201 atatggatga agtgccgcag gcagagccat ggcagaatgc gacgggccat cccatcggtt
     4261 ccgcactgta ctataagacc gtcgggatat tcaaggatca ggcagacctg acgagaaatg
     4321 ccaatgaagc cggaagcggc agccaggtgg gggatcagaa agtagcgaac ctgagcggcg
     4381 gaacacagat caccgcgaat gatcaattcc gttttgacta ttcgcccatc ccgatggaga
     4441 cctttggttt cacttcggta ttccgttata aatcctggga cctgaacctg ttcttctacg
     4501 ggcagtcgaa ggtgtacaac tacgatgcga tatacgccaa tctcggtagt gcggatttct
     4561 ccaacgccat ggtggcgagg gcaaccaacc gctggtcggt gagcaatccg aatggatccc
     4621 ttccgcgctc cggcgaatgg acaccggggg gaaccacttt gtacctgaag gacgccacct
     4681 ttatcaggtt gaaaacggcg gagatcggtt acaatttacc gcataacctg ctcgccagga
     4741 ccaagacctt taccgatttt cgcgtatacg taagcggctt caacgtgctg acctgggcga
     4801 aggccatcaa gtacgccgac cctgagatca acgggaactc cactgcgtac ccgcagcaaa
     4861 ggatcatcaa ttttggatgt actgccaggt tttaaaaaag ataattttta aaaatacgtt
     4921 ttatgaaaaa gatttttgtc ataatcatat tatccggctg tctggcggcc tgccgcagga
     4981 atgtgctgga tattcctccg caggacagga tctcggagaa tgccgtatgg acggacccca
     5041 acctggttgc tgcctatgag aacaacctgt
//
LOCUS       DATGFG010000500         4221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1228168, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000500 DATGFG010000000
VERSION     DATGFG010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228168"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VKQ52_09230"
     CDS             <1..833
                     /locus_tag="VKQ52_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87412.1"
                     /translation="LENIGGLSNKGIEITLSGKPVVTKDFSWTVTFNIAHNTNRVTAL
                     YKNNPVPSPNYYFQYTVGHDFQTYYLPQWGGANSQNGLPQWYTDNTKKTLTNGYDSAT
                     FVLNDNYTATPKVFGAFTNNFTYKGFYLDIQFNYNFGNYLFDTWGFITNSEGSFLGGY
                     NQMTSQLRAWQKPGDKTDIPQIVFGGNDNSTNLSTRYLYKGNYIRMRNLQFGYTIPKS
                     AVDRLHLSSISVYIRGTNLLTFATDKNLPYDPESGVASSTNFEIFIPKTIAGGIKIGF
                     "
     gene            852..2276
                     /locus_tag="VKQ52_09235"
     CDS             852..2276
                     /locus_tag="VKQ52_09235"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87413.1"
                     /translation="MKKILNKYTLGCLSLVLVVAGCSKNFINKTPNDSLLTSQALGDA
                     NALQSALNGAYNQLGQVSVYGRDLPVVGDLMADNTYVQLKNSNRYISQYNYSVTKLDA
                     VPSEVWPGCYAAILRANQIIDSKLTGTKVNEIKAQAYAIRALMYFKLVTYFAKPYTTD
                     TTALGVPLVLHYAPYALPTRNTVGTVYNQMVSDLKAAMPIAPAYVNSVTLNKYAIEGL
                     LARVYLYMADYKDAEAAATDVINNSGFSLVSAANLPAFWANPGVQTDQTEVMFEVNQD
                     VLVNNGFDDFGGIYFNGYADLYCSGQLYNLYSQTDARIALIDSGTTAAGTFAWQVVKF
                     PNAGNSDKDNLKVIRLAEVYLIGAESAARLNDPTTAQGWLNTLMTNRDPAFAGYSDIG
                     QTLINDIIQERRKELAFEGDRFFDLNRLGLQINRAANAGALPAGIGNVNLTIAWPDNR
                     RVAPIPNAEIQANPNIASQQNPGY"
     gene            2423..3730
                     /locus_tag="VKQ52_09240"
     CDS             2423..3730
                     /locus_tag="VKQ52_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87414.1"
                     /translation="MKYLFGLLLVLAAAPGWAQTPWEDIYTDFVLYNKRVALEKDLRE
                     RVVGRHFSAPLDSSTEDGYLSACWAVEQFLFDGPVVMQGFDHLFAGYASLSDDTKRAL
                     LEAVYAVAPERYRQEMEAVLGKEENPRLFALCAVYLFRADTSREQANTLKIRMVERFP
                     GYDTIPVLQVLQHFLSFTRPKLPPIGELFAWQQQSDGRRNQRGAIGPTGGGAKTIYSF
                     QRWNRDYAGLAIVQNADGSFVRDGDGRLQVFEQLARSGPGLPYFLTNGNTPQGIYSIQ
                     GTDVSRTNFIGPTPNLQLLLPGEGKWGDYFQDPDSAAGDSLGCYLALLPPGWRGYAPM
                     MEAWAAGAIGRTEIIAHGTTIDPEYFRGRPFYPLTPTMGCLCAKELWNPTTGHLLVSE
                     QNNLVNAFLSTPGRKGFLIVVNIDDQRRAVSREEVEAWVRKRG"
     gene            complement(3797..>4221)
                     /locus_tag="VKQ52_09245"
     CDS             complement(3797..>4221)
                     /locus_tag="VKQ52_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLZ87415.1"
                     /translation="IGNLGKDCVTNMVNGKNVINFNVAHTEKFRDAQGNQKDKTIWVE
                     CAYWTDRTGIAPYLKKGTQVYAEGTPEVRTYPKNDGTTGVSLTLRVQSVQLLGSRGSE
                     GGGGYSGGGSEGYSSPQAGAARVPSASDITEPIDDLPF"
BASE COUNT          929 a         1126 c         1247 g          919 t
ORIGIN      
        1 aactggagaa catcggcggt ttatcgaaca agggcatcga gatcacgctc agcggaaagc
       61 ccgttgtcac caaggatttc agctggacgg tcaccttcaa catcgcgcat aacacgaacc
      121 gcgtcaccgc cctctacaag aataatccag tccccagccc caactattat ttccagtata
      181 cggtgggcca tgatttccag acctactacc tgccgcaatg gggcggagcc aattcgcaaa
      241 acggtctgcc gcaatggtat accgacaaca ccaagaagac gctcaccaac gggtatgaca
      301 gcgccacctt tgtgttgaac gacaactata cggctacgcc aaaggtattc ggggccttta
      361 cgaataactt tacgtataag ggcttttacc tggatatcca gttcaactat aatttcggga
      421 actacctgtt cgatacctgg ggattcatca ccaactccga aggctccttc ctcggaggat
      481 acaaccagat gacgagccaa ctgagagcct ggcagaaacc cggcgacaag acggatatcc
      541 cacagatcgt cttcggcggg aacgacaatt ccaccaatct ctccacgcgt tatctctaca
      601 agggtaatta tatccgcatg cggaatctgc agttcggcta tacaatcccg aagtcggccg
      661 tggacaggct gcatctgtcc agcatcagcg tatatatccg gggcacgaac ctgctgacct
      721 ttgccaccga taagaacctg ccttatgatc ccgaatccgg tgtagccagc agtaccaatt
      781 ttgagatttt tatccccaag acgatcgcgg ggggcattaa aatcggcttt taatctttaa
      841 aacacacgga aatgaaaaaa atactcaata aatatacact gggctgtctt tccctcgtat
      901 tggtcgtggc gggttgcagt aaaaacttta tcaacaaaac accaaacgat tccctgctca
      961 cgagccaggc cctcggcgat gccaatgccc tgcagagtgc gctgaacggc gcctataacc
     1021 aactgggcca ggtgtccgtt tatggccgcg atctcccggt ggtcggcgat ctgatggccg
     1081 ataacaccta tgtccagttg aagaactcca accggtatat ttcccagtac aattacagtg
     1141 ttacgaagct ggatgccgtc cccagtgagg tttggccggg ctgctatgcc gccatcctgc
     1201 gcgccaacca gatcatcgat tccaaactga cgggcacgaa ggtgaacgag atcaaggcgc
     1261 aggcctatgc catccgcgcc ctgatgtatt tcaagctggt cacctatttc gcgaagccgt
     1321 acaccacgga taccaccgcc cttggcgtcc ccctggtcct tcactatgcg ccgtatgcgc
     1381 tgccgacgcg caacacggtc ggcaccgtct acaaccagat ggtcagcgat ctgaaagccg
     1441 cgatgcccat tgccccggca tatgtcaatt cggtgaccct gaacaagtac gccatcgagg
     1501 ggctgctcgc aagggtctat ctttacatgg cggattataa ggatgcggaa gccgccgcaa
     1561 cggatgtcat caacaacagc ggtttcagcc ttgtctccgc ggccaacctg cccgcgttct
     1621 gggcgaaccc gggagtgcag accgatcaaa cggaggtgat gttcgaggtc aaccaggatg
     1681 tgcttgtcaa caacgggttc gacgattttg gcggcatcta tttcaacggg tatgccgacc
     1741 tgtattgcag cggccagctc tacaacctgt atagtcaaac cgatgcccgt atcgcgttga
     1801 tcgatagcgg cacgacggca gccggcactt ttgcctggca ggtggtcaaa ttccccaatg
     1861 cggggaactc cgacaaggat aacctgaagg tgatccgcct ggcggaagta tatctcatcg
     1921 gggcggaatc ggcggcccgg ctgaacgatc ctaccacggc gcagggctgg ctgaatacac
     1981 tgatgaccaa ccgggacccc gcctttgcgg gttactcgga catcggtcag accctgatca
     2041 acgatatcat ccaggagaga aggaaggagc ttgcatttga aggcgaccgg ttcttcgatc
     2101 tgaacaggct gggcctgcag atcaaccggg ctgcgaatgc cggagcgctc cccgcgggga
     2161 tcggcaatgt aaatctgact attgcctggc cggataaccg gcgggtggcg ccgatcccca
     2221 atgcggagat ccaggccaac cccaatattg cgagccagca gaatccagga tactaaggcc
     2281 cgggccttcg gaactacccg gcgataaacc tacagtacaa aggccgctcc tcgttggggc
     2341 ggcttttgtt ttgagacgac ctgcttccgg aggcttttgt tttgtggcgg cccgcttccc
     2401 gcggcttttg tattttcgtg ggatgaagta tttgtttggg ttgttgctgg tgctggcggc
     2461 ggctccgggt tgggcgcaga ccccgtggga ggatatctac acggattttg tgttgtataa
     2521 caagcgggtg gcattggaga aggatctgcg ggagcgtgtg gttggccggc atttttcggc
     2581 gccgctggac agcagtacgg aggatggcta tttatcggcg tgctgggccg tggagcagtt
     2641 cctttttgac ggaccggtgg tgatgcaggg tttcgatcat ttatttgccg gctatgcgtc
     2701 gctctccgat gatacgaaac gggccctgct ggaggcggtg tatgcagtgg cgccggagcg
     2761 ttaccggcag gagatggagg ccgtgttggg gaaggaagaa aacccgcggc tttttgcgtt
     2821 gtgcgccgta tacctgttcc gggcggacac gagcagggag caagccaata ctctgaagat
     2881 caggatggta gaacggttcc cggggtatga cacaattccc gtgctgcagg tgttgcagca
     2941 tttcctgagt tttacccggc cgaagctgcc ccccatcggg gagctgtttg cctggcagca
     3001 gcagtcggac gggcgccgaa atcagagggg cgccatcggt ccgacgggag gaggggccaa
     3061 gacgatctat tcctttcagc ggtggaaccg ggattatgcg gggctggcga tcgtacagaa
     3121 tgcggatggc agttttgtgc gggatgggga cgggcggttg caggtgttcg aacaattagc
     3181 gcgatcggga cccggtctgc cttatttcct gacgaatggg aatacgccgc agggtatcta
     3241 tagcatacag gggacggatg tgtcgaggac gaactttatc ggtccgacac caaacctgca
     3301 gctcttatta cccggcgagg gaaaatgggg ggattatttc caggatcccg attcggcggc
     3361 gggtgactcg ctggggtgct acctggcgtt gttaccgccg ggatggcggg gatatgcgcc
     3421 gatgatggag gcctgggccg cgggggcgat cgggcggaca gagatcattg cgcatggcac
     3481 gaccatcgat ccggaatatt tcaggggaag gcctttctat ccgcttacgc cgacgatggg
     3541 ctgtctgtgc gccaaggagt tatggaatcc aaccaccggg catttgctgg tgagtgagca
     3601 gaacaacctg gtaaatgctt ttttgtcgac gccgggaagg aagggtttcc tgatagttgt
     3661 caatatagat gatcagcgga gggcggtgag ccgggaggag gtggaggcgt gggtgcggaa
     3721 gcgcggataa gggcctggcg gggatgagac aagtcaaaga aaaacccccg atgagcatcg
     3781 ggggtttcgg gatgatttag aaaggaagat cgtcgatcgg ctcggtgata tcgcttgccg
     3841 acggtactct tgccgcgccc gcctgggggg aagaataacc ttcggagccg ccgccggaat
     3901 agccgccacc gccttcggag cctctgctgc ccagcagctg cacggactgt acgcgcaggg
     3961 tgagggagac accggtggtg ccgtcgttct tgggataggt ccttacttca ggagtacctt
     4021 ccgcatatac ctgggtgcct tttttaagat agggggcgat gcccgtcctg tccgtccaat
     4081 atgcacattc cacccaaatc gttttatctt tctgattgcc ctgggcatcg cggaatttct
     4141 cggtgtgtgc cacgttgaaa ttgatcacgt tcttcccatt caccatattg gtgacacagt
     4201 cttttcccag attgccgatt a
//
LOCUS       DATGFG010000501         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1264826, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000501 DATGFG010000000
VERSION     DATGFG010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264826"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VKQ52_09250"
     CDS             <1..381
                     /locus_tag="VKQ52_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLZ87416.1"
                     /translation="LKEGGPYKGKRITFHDSCYLGRANDIYEAPRKVLEALEVELVEM
                     KRCRSKGLCCGAGGAQMFKEEEKGQTRVNFERSGEALGTGAGVVAAACPFCNTMLTDG
                     VKHAEKEAAVQVLDVAELIAASID"
     gene            415..945
                     /locus_tag="VKQ52_09255"
     CDS             415..945
                     /locus_tag="VKQ52_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87417.1"
                     /translation="MNSHHSELIPGDFAPESRVWIYQSPRLFFLSEALEIEKMLETFV
                     ADWKSHGTPVKGYANLFYGQFIVLMADETATGVSGCSTDSSVRLIRDIEERFGMPLFD
                     RLMLAFRLKDKVETVPLAQLSHALENGFIGPDTLYFNNTVQTKVELESKWLIPLKDSW
                     LAGRYLKAEGTRTPGK"
     gene            1050..1122
                     /locus_tag="VKQ52_09260"
     tRNA            1050..1122
                     /locus_tag="VKQ52_09260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1083..1085,aa:Gly,seq:ccc)
     gene            complement(1154..2878)
                     /locus_tag="VKQ52_09265"
     CDS             complement(1154..2878)
                     /locus_tag="VKQ52_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLZ87418.1"
                     /translation="MFRHPLFTGCILMLLAALLPSSGSAQARGTDTISYSSTCVNSTI
                     LFSSPLLDTFYTPDYIEWHFSDPASGYNDSSGANKPRHLFAATGQYPVELDVWYKGSD
                     TIKIRDTITIVTPMNFNFGPDIYVCGKKPDTLISGPNIPGATYTWNDADTTHTDTIRI
                     TKSGIYTVAVNGCGVTDSIGVFSSDTPRLDLGKDHIMCDSANLQLDAASQNGRYTWLL
                     DGAALPDTGDQLITHYPGGTYVAIVTVPGCGVYSDTVSITYSQPLAPPFDLGPDTLLC
                     PKQVFTLDAAIPGATAYDWNTGSTDSVITITATGDYFVFVTYKNQCQVTDSVLVTYRD
                     DKPLDFHDTAICKGSTLVLNADFGQGTYKWTAIPPQRDDQNQTGQSTYFVYEPGKYAV
                     LAQVGQCVYTDTLTVRFDDSLRVTMPKDTTLCYGEDFLLRVAGNADTLVWQDGSYGDA
                     YRPAQPGIYTVVARNGCGTDTLTATVNFTTCGCQLLLPNAFTPDGDGHNDTFRPLHAC
                     EMTNFEMTIYNRYGELVFRSVNPDDAWDGRFRGARVQGGAYVWMARYFNPVTKQPVFR
                     KGTVLVIR"
BASE COUNT          616 a          769 c          944 g          645 t
ORIGIN      
        1 ctgaaggaag ggggtcccta taaaggcaaa cggataacct tccacgacag ctgctatctt
       61 ggccgggcca atgatatcta cgaggcgccc cgcaaggtgc tggaagccct cgaagtggag
      121 ctggtggaga tgaaacgctg ccgcagcaag gggctttgtt gcggggcagg cggcgcccag
      181 atgttcaagg aagaggagaa ggggcagacc cgggtgaatt tcgaacgcag cggcgaggcc
      241 ctgggtacgg gggcgggtgt ggtcgcggct gcctgtcctt tttgtaatac gatgctgacc
      301 gatggcgtca aacacgcgga aaaggaggcg gcggtacagg tgctggatgt cgccgaactc
      361 atcgcggctt cgatcgatta gccgattgcg gccgggattg cttaattttg tgtcatgaat
      421 tcccatcact ccgaactgat acccggcgat ttcgcgccgg aatcgcgggt ctggatctac
      481 cagagccccc ggcttttctt cctgtccgag gccctggaga tcgaaaaaat gctggaaacc
      541 tttgtcgccg actggaaatc ccatggcacc ccggtgaagg gctatgcgaa cctcttttac
      601 gggcaattca tcgtgctgat ggccgatgag acggccaccg gggtcagcgg ttgcagcaca
      661 gacagcagcg tccgcctgat cagggatatc gaggagcgct ttggcatgcc gcttttcgac
      721 cggctgatgc tggcgttccg gttaaaggac aaggtggaga cggtccccct ggcccagttg
      781 tcccacgccc tggaaaatgg gtttatcggg cctgatacgc tctatttcaa caataccgta
      841 cagactaaag tggaattgga gtcaaaatgg cttattccat tgaaggatag ttggttggct
      901 ggccgctatt taaaggcgga gggaacccgg acgcccggaa aatgaacttt accggaaaat
      961 gggcgggtta gcgaaaaaga tgagaagtta ttttattgat acccggcgaa cccttacatt
     1021 tgcgtcccgg ttaaaaatcg gcaagatctg cggaagtagc tcagttggta gagcgacagc
     1081 ttcccaagct gtaggtcgcg ggttcgaacc tcgtcttccg ctcaaaggac acttgatggt
     1141 gtcctttttt tatttacctg atcaccagca ccgtcccctt acggaatacg ggttgtttgg
     1201 tcacggggtt gaaatacctc gccatccaca cgtaggcgcc gccctggacc ctggcgccac
     1261 ggaaacggcc gtcccaggca tcgtcggggt tgacgctgcg gaagacgagt tcgccgtagc
     1321 ggttgtagat agtcatctcg aaattggtca tttcgcaggc gtgcagcggc cggaaggtat
     1381 cgttgtgccc gtcgccgtcg ggggtaaagg cattgggcag cagtagctgg cagccgcagg
     1441 tggtaaaatt gacggttgcc gtcagcgtgt ccgtaccgca cccgttgcgg gccacgacgg
     1501 tatagatacc gggctgggca ggccgatagg catcgccata gcttccatcc tgccagacaa
     1561 gggtgtctgc attacctgct acccgcagca ggaaatcctc gccatagcaa agcgtggtat
     1621 cctttggcat cgtcacccgc aggctgtcgt cgaagcgtac ggtgagcgtg tcggtataga
     1681 cgcattgccc cacctgggcg aggacggcgt atttgcccgg ttcgtagacg aaatacgtgg
     1741 attgtcccgt ctggttctga tcgtcgcgct gtggtgggat ggccgtccat ttgtaggttc
     1801 cctgcccaaa atcggcattc agcaccagcg tgctgccttt gcagatggcg gtgtcgtgga
     1861 aatcgagcgg cttgtcgtcc cggtaagtga ccagtacgct gtcggtgacc tggcactggt
     1921 tcttataggt gacgaacacg aaatagtccc cggtggcggt gatcgtgatg acgctatcgg
     1981 tagagccggt gttccagtca tacgccgtcg cgccgggaat cgccgcatcg agggtgaaca
     2041 cctgtttagg acagaggagg gtatcggggc cgaggtcgaa gggaggcgcc agcggctggg
     2101 aataagtgat ggagacggtg tcgctataga cgccgcagcc gggaacagtc acgatcgcca
     2161 catacgttcc gccggggtag tgcgtgatca gctgatcccc ggtgtcgggc agcgcggccc
     2221 catcgagcag ccaggtgtac cgcccgttct gcgaggcggc atcgagctgc aggttggcgc
     2281 tgtcgcacat gatatggtcc ttgcccaggt cgagccttgg cgtgtcgctg gaaaatacgc
     2341 cgatgctgtc ggtcaccccg cagccgttga cggcgaccgt atagatgccg gatttcgtga
     2401 tcctgatagt atcggtatgc gtggtatcgg catcgttcca ggtgtaggtg gcgccgggaa
     2461 tattgggtcc gctgatcagg gtgtcgggtt tcttcccgca gacatagata tcgggaccga
     2521 aattgaaatt catcggcgtt acgatcgtaa tggtgtccct gatctttatc gtatcgctgc
     2581 ccttgtacca gacgtccaac tccaccgggt attggcctgt ggccgcaaac agatgccggg
     2641 gtttgttcgc gccggagctg tcgttatagc ccgacgccgg atcactgaaa tgccattcga
     2701 tgtagtcggg tgtgtaaaag gtgtccagca gcggcgagga aaaaagtatc gttgagttga
     2761 cacaggtgga ggaatagctg atcgtgtccg tccccctggc ctgggcagac ccggaggaag
     2821 gcagcaatgc cgccagcagc ataagaatac agccggtaaa aaggggatgc ctgaacatcg
     2881 gcctggtcat tagtgatgaa cttttaatag ggttcttacc tgttgtaccg attgattctg
     2941 cagccgggca taatagaccc cgtcggccag gaag
//
LOCUS       DATGFG010000502         6551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1652235, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000502 DATGFG010000000
VERSION     DATGFG010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652235"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VKQ52_09270"
     CDS             complement(<1..548)
                     /locus_tag="VKQ52_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87419.1"
                     /translation="MKLFKRRVVIFSVLFCGCFLAGGQAQAQLRDYLNLPDHDTKPYY
                     IGIGAMYVTSHLQVTAHPKFLQSDSILYVTPGNSGGFGLSGMFTFRLAEHFEFRFAFP
                     EFIFANNTLAYHVNNPPAGETNVATKQIQSLLLGFPAQVKFLSDRINNFRVYMLGGVN
                     YRYDLASNSSARKAQNLVKLVPT"
     gene            complement(676..1476)
                     /gene="ubiE"
                     /locus_tag="VKQ52_09275"
     CDS             complement(676..1476)
                     /gene="ubiE"
                     /locus_tag="VKQ52_09275"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HLZ87420.1"
                     /translation="MANYSHDRIVPFKDSQMGKKQQVAEMFDKIAFRYDFLNRFLSGG
                     IDQYWRRRAIGEVRIAIGKDRVGTVAPTATTADATRSILDVATGTADMAIMMARHLHA
                     VRITGVDISTGMLEIGRQKIAKAQLGERIILQTGDSESLQFPDETFDAITVAFGVRNF
                     ESLEKGLKEMLRVLKPGGKLVVLEFSQPRTPGIRQCYDLYLRLVAPNVGKMISSSREA
                     YQYLNDSVRAFPEGAAFVRILDGCGYSNTRLRRLSLGICTLYTGQKQG"
     gene            1498..2181
                     /gene="yihA"
                     /locus_tag="VKQ52_09280"
     CDS             1498..2181
                     /gene="yihA"
                     /locus_tag="VKQ52_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HLZ87421.1"
                     /translation="MKVSNELWGEAPYLTALLCIMDIHSSVYVISSPDHTQSPKPDRP
                     EYAFIGRSNVGKSSLINMLCNNEKLAKTSGTPGKTQLINHFSINDEWYIVDLPGYGFA
                     KVSQSQRKKWEKMIEDYLRRRENLVNVFVLIDSRHSPQKIDLEFVNRLGEWQVPFTLV
                     FTKADKENQRVVSANVKAFLGKMRETWQFLPQHFVTSTLKKMGRDKILKLIEEMNGEF
                     EEGRRSPGS"
     gene            complement(2243..2716)
                     /locus_tag="VKQ52_09285"
     CDS             complement(2243..2716)
                     /locus_tag="VKQ52_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HLZ87422.1"
                     /translation="MLWRKFNGDVIELPIIEAVENAIRRETEKGYRLKVCIGTDSQVK
                     GLETEFATVIVFLREGHGGFMFIHNEKTRHQYTIKERMLVEVAKSIEIAYELCNLFSK
                     YNVDMEVHADINTNPHFKSNDALKEAMGYILGMGFAFKAKPEAFASSSCANKVVN"
     gene            complement(2787..3515)
                     /locus_tag="VKQ52_09290"
     CDS             complement(2787..3515)
                     /locus_tag="VKQ52_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HLZ87423.1"
                     /translation="MKKYFAEFLGTFSLVLFGCGAAVVSGKTIPDAVLAGAPAGIGVL
                     GIALAFGLAVVVMCYAIGPISGCHINPAITIAMLIAGKISTKDSVGYIIAQFAGALLG
                     AAVLYAFQQGAPDFSMGEWALGSNGWGPGYLGNYSTSSAFMAEAVLTFLFLFVIFATT
                     SRLGSGPMSGLAIGLTLVLIHLVAIPITGTSVNPARSFGPALFAGGKALQQLWLFIVA
                     PLAGAIVAAGVWRGLFETKDKQGA"
     gene            complement(3549..3929)
                     /locus_tag="VKQ52_09295"
     CDS             complement(3549..3929)
                     /locus_tag="VKQ52_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HLZ87424.1"
                     /translation="MNDMSQTAKKTLVLGASDNPQRYSYLAMTRLRSNGHPVVAIGRK
                     PVTVGDIPVETEKKAFPGVDTVTLYLNPRSQKEYYEYILSLHPKRIIFNPGAENEELL
                     RLAQDNGIQPLEACTLVLLSTGQY"
     gene            complement(4029..5018)
                     /locus_tag="VKQ52_09300"
     CDS             complement(4029..5018)
                     /locus_tag="VKQ52_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HLZ87425.1"
                     /translation="MKNKKIVIAGGTGFIGQALAARWAKDNQVVILSRQSASATNNSY
                     SHRLLTAADGYNITYWHWDGRFVEKHWADELEGCDLVLNLAGRSVNCRYTERNKQEIS
                     DSRADATRVIGQAIRETVVPPKLWVNGGSATIYRHAQDRPQDEYTGEIEDDFSVRVCK
                     RWERTFEEQRTPFTRKIALRLAITLGEGGVVIPYLNLLKYGLGGPQGDGRQMYSWVHI
                     DDVGRAIEWFLEHPELEGVYNVAAPGPITNRFFMTTFRRLTGRRIGLRAPALLLKLGA
                     LLIGTETELVLKSRWVLPTKLQETGFRFQYERIEPALADIVARVGAPGDAARS"
     gene            complement(5266..5781)
                     /locus_tag="VKQ52_09305"
     CDS             complement(5266..5781)
                     /locus_tag="VKQ52_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87426.1"
                     /translation="MTLAEAKAQFIADWGRFGTNWGINRTMAQIHALLLVSPDALSAD
                     EVMEQLSISRGNANMNLRELIDWGLVQKVLIPGERKEFFSAEKDIWKVARQIIKERKK
                     RELDLMIPVLKELSEVEGDKRDKAVKTFTDTINNIRKFSEQADKTLDTMIKADEHWFL
                     GSLVKLFAGKN"
     gene            complement(5927..>6551)
                     /locus_tag="VKQ52_09310"
     CDS             complement(5927..>6551)
                     /locus_tag="VKQ52_09310"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HLZ87427.1"
                     /translation="YDDEDDYYEYEWKIGVSLLDLGGNNYQYGSQSRTAGNPKSSVSD
                     VDLNQKFNYVGSMAQFNDSLATIVNSMAPLTGHFIIWNPARLVINVDRQLPEHFALNT
                     ELTLNLGGDNTAKRLFTKEITLFALTPRWEKKNLGGYLPLTVTTDGKVWVGGAVKAGP
                     LLLGVHNWANVFSKTKTQNGGFYLALMIRPGKGFRFKEAKEYTCPKN"
BASE COUNT         1387 a         1823 c         1833 g         1508 t
ORIGIN      
        1 gtggggacca gttttaccag attctgcgcc ttgcgcgcgg aggaattgga ggcaagatcg
       61 tagcggtagt taacgccgcc cagcatgtat acgcggaagt tattgatccg atcggacagg
      121 aacttcacct gcgcgggaaa acccagcagg agcgattgga tctgtttggt cgcgacattg
      181 gtctctcccg cgggcgggtt gttgacatgg taggcgaggg tattattggc aaaaataaac
      241 tccgggaagg caaaccgaaa ctcgaaatgc tcggcaagcc ggaaggtgaa catgcccgac
      301 aggccaaaac caccggaatt gccgggtgtg acatacagga tgctgtcgct ttgcaggaat
      361 ttcggatggg ccgttacctg caaatggctg gtgacgtaca tcgcgccgat gccgatataa
      421 tacggttttg tatcgtgatc gggcaggtta agataatccc tcaactgggc ctgcgcctgg
      481 ccgcccgcca gaaaacagcc gcaaaacagt acggaaaaaa tgactaccct acgcttgaat
      541 aacttcataa actatttaaa atcagtccaa taaaacgttc ctggaaagat tgaccaggat
      601 ttcagggtag atactctcga taggcttgat ttgaagtggt acacataacg aaaaaaccgc
      661 cggttaattg cccttttacc cctgtttctg cccggtatac agcgtacaga tgccgaggct
      721 gagccggcgc aaccgggtat tcgaatagcc gcagccatcc aggatccgga cgaaggccgc
      781 cccttcgggg aaggcccgga cggaatcgtt caggtattga taggcttccc ggctgctgga
      841 tatcattttt cccacgttgg gggccaccag ccggaggtag agatcgtagc attgccggat
      901 gccgggtgtc cgcggttgag agaattccag caccaccagt ttgccacccg gtttcagcac
      961 ccgcaacatc tccttcaatc ctttctcgag gctttcgaaa ttgcggacgc caaaggctac
     1021 cgttatcgcg tcaaaggttt catcgggaaa ctgcagggat tcgctgtcgc ctgtctgcag
     1081 gatgatcctt tccccgagct gcgccttcgc gatcttctgc cggccgatct ccagcatgcc
     1141 cgtggaaata tccacccccg tgatccgcac ggcgtgcaaa tggcgggcca tcatgatggc
     1201 catgtccgcg gtgcccgtag ccacatcgag gatcgaccgg gtcgcatcgg ccgtagtcgc
     1261 ggttggcgcg acggtcccca cccggtcttt gccgatggcg atgcgcacct ccccgatggc
     1321 ccgccgccgc cagtactggt cgatcccgcc ggacagaaag cggttaagga aatcataccg
     1381 gaaggctatc ttgtcgaaca tctcggccac ctgctgcttc ttgcccattt gtgaatcctt
     1441 gaacgggacg atcctgtcat gcgaataatt agccatagaa cggcgaaatt acgaatcttg
     1501 aaggtaagca acgaattgtg gggcgaagct ccgtatctta cggccttatt gtgtattatg
     1561 gacatccatt cctccgtcta tgtgatcagc agtcccgacc acacgcaaag tccgaagcca
     1621 gaccggccgg agtatgcgtt tatcggccgg agcaatgtcg gcaagtcatc gctgataaat
     1681 atgctttgca acaatgaaaa actggcaaag acctccggta cgccgggcaa gacccaactg
     1741 atcaatcatt tcagcatcaa tgacgaatgg tacatcgtgg atctgcccgg ctatggcttt
     1801 gccaaggtgt cccagtcgca gcggaagaaa tgggagaaga tgatcgagga ttatctccgg
     1861 cgccgcgaga atctggtaaa tgtctttgtg ctcatcgaca gccggcactc cccacagaag
     1921 atcgacctcg aattcgtcaa ccggctgggc gagtggcagg ttccctttac ccttgtcttc
     1981 acaaaagcag acaaggagaa ccagcgtgtc gtcagcgcca acgtaaaggc ctttctgggg
     2041 aagatgcgcg aaacctggca gttcctgcca caacattttg tcaccagcac gttaaagaaa
     2101 atggggaggg ataagatcct gaagctgata gaggagatga acggggagtt cgaagagggg
     2161 agacgatccc ccgggtcgtg aatcgggggc acgaccgttg accgtcgggg gctaaatgat
     2221 ggccctgggc ggcagtcagg gtctaattga ccactttatt tgcgcagctg ctgctcgcaa
     2281 aggcttccgg cttggccttg aaggcgaagc ccataccgag aatgtagccc atcgcttcct
     2341 tcaatgcatc attgctcttg aaatgcggat tcgtattgat gtcggcgtgt acttccatgt
     2401 cgacattata cttgctgaag agattgcaga gctcgtaggc aatctcgatg ctctttgcca
     2461 cttcgaccag catccgttcc ttgatggtgt actgatgccg ggtcttctcg ttgtgaatga
     2521 acatgaaccc gccatgtcct tcgcggagaa aaacgatcac ggtagcaaat tccgtttcca
     2581 aaccttttac ctgggagtcg gtgccgatac agactttcag ccggtaacct ttttcggtct
     2641 cgcggcggat tgcgttttca acggcttcaa tgatgggaag ttcgatgaca tctccattaa
     2701 attttcgcca tagcatacta taaaggattt ttcgaattta ccgaaaaagg ctttattgac
     2761 aaaatcgtat ggagattaag cttttattaa gcaccctgtt tgtcctttgt ttcgaacagg
     2821 cctctccata cccctgcagc cacaatggct cccgccagcg gggcgacgat gaacaaccaa
     2881 agttgctgca aagccttgcc gccggcgaac agcgcgggcc caaagcttcg tgcgggattt
     2941 acggatgttc ccgttatagg gatggctacc aggtggatga gcacgagggt caggccgatg
     3001 gcgagcccgc tcatcgggcc gcttcccagc cgggaggtag tggcaaaaat cacgaagaga
     3061 aacaggaacg tgagcacggc ttccgccatg aacgccgagg aggtgctata gttgccaagg
     3121 tatcccggcc cccagccatt tgagcccagc gcccattctc ccatgctgaa atcaggcgca
     3181 ccctgctgaa aggcatataa caccgccgca ccaagcaggg cgccggcaaa ctgggcgatg
     3241 atatacccga cggaatcctt tgtgctgatc tttcccgcga tcaacatggc gatagtgatg
     3301 gcggggttga tatgacatcc cgaaatggga ccgatggcat agcacatgac caccaccgcg
     3361 agcccgaatg ccagggcgat accgagcacg ccgataccgg cgggtgcgcc tgccaataca
     3421 gcgtcgggaa tcgttttccc cgataccacc gcggcgccac agccaaacag gactaacgag
     3481 aaggttccca ggaattcggc aaaatacttc ttcatatgat agtgtttatc agctaaagta
     3541 agtatttttt agtactgtcc tgtgcttaac agcaccagcg tacaggcctc caggggttgg
     3601 atgccattgt cctgcgccag ccgaaggagt tcttcgttct ccgcaccggg gttgaagatg
     3661 atcctttttg ggtgaagcga gaggatatac tcgtagtatt ccttctgtga gcggggattg
     3721 aggtagaggg tcacggtatc gaccccggga aacgcctttt tctccgtttc gaccgggata
     3781 tctcccacgg ttaccggctt ccggccgatg gccaccaccg ggtgtccgtt gctgcgcagc
     3841 cgggtcatgg ccaggtagct ataccgctgc gggttatcgg aagccccgag gaccagtgtc
     3901 ttttttgccg tctgggacat atcattcata caatcggcag cgctttttgt tcactgcgat
     3961 cgccgccgtg cgcgggatcc cgggtatggc cgccgttcgt gggatcccgg atattatgat
     4021 gaaacccgtt atgaacgagc tgcatctcca ggggcgccca ccctggcgac gatatcggcc
     4081 agcgccggct ctatccgctc gtattggaac cggaatcctg tttcctgcag ctttgtcggc
     4141 aacacccagc ggctttttaa tacaagctcc gtctcggtgc cgatgagcag ggcgcccagt
     4201 ttcaacagga gggcaggtgc gcggagcccg atccggcggc cggtcagccg gcgaaaggtt
     4261 gtcatgaaga agcgattggt gatggggccg ggcgccgcga cattgtagac accctccagt
     4321 tccgggtgtt cgaggaacca ctcgatagcg cggcccacat catcgatatg cacccagctg
     4381 tacatctggc ggccatcgcc ctggggcccg cccagcccgt atttcagcag gttgagatag
     4441 gggatgacca ctccgccttc ccccagtgtg atcgccagcc gcaaggctat cttgcgggta
     4501 aagggagtac gttgttcttc gaaggtgcgc tcccagcgtt tgcatacccg cacggaaaaa
     4561 tcatcttcga tctcacccgt atactcgtcc tgcggacgat cctgcgcgtg ccggtagatg
     4621 gtagcggagc ctccgttgac ccagagcttt ggcggaacta ccgtctcccg gatggcctgt
     4681 cctatcaccc gcgtggcatc ggcccggctg tcggaaatct cctgtttgtt gcgctccgta
     4741 taacggcagt tgacgctgcg cccggccagg ttgaggacga ggtcgcagcc ttccaactcg
     4801 tcggcccagt gcttctcgac aaaacggcca tcccaatgcc aatacgtaat attgtatcca
     4861 tcggctgccg taaggagccg gtggctatag gagttgttgg tggcggaggc agactgacgg
     4921 cttaggatca ctacctggtt gtctttcgcc cagcgggcag ctaatgcctg accgataaag
     4981 cctgtgccgc cggcaatgac gatcttctta tttttcatgt tcattgtttt tgaagtttca
     5041 ataaatattg aaatattggg gtcaaataaa agccgcataa cggcttgttt gggtggtggc
     5101 cggggggtcg gcctggcggg ccggtgggtc ggcggctcag cgaggcggat cggtggatcg
     5161 gcgggtaagc caggcgggtc gatgggtcgg cctcagccag gcgggtcgat gaggcgggtc
     5221 ggccggtagg ggctcgtggc ggctcctggg tctcgcggcg gcacctcaat tcttcccggc
     5281 gaacagcttc accaggctgc ccaggaacca gtgctcatcc gccttgatca tggtatccag
     5341 cgttttgtcg gcctgttcac tgaatttgcg gatattgttg atcgtatccg taaaggtctt
     5401 gaccgcctta tcccgcttat cgccttccac ctcgctcagc tccttcagca cagggatcat
     5461 cagatcgagc tcccgtttct tccgctcctt tatgatctgc cgggccacct tccagatgtc
     5521 cttctcggcg gaaaagaatt cctttctctc cccggggatc agcaccttct gcaccagccc
     5581 ccaatcgatc agctcccgca ggttcatgtt cgcattgccc cggctgatcg acagctgctc
     5641 catcacctcg tccgcgctca gggcatcggg actcaccagc aacagcgcat ggatctgcgc
     5701 catcgtccgg ttgatgcccc agttggtgcc gaacctgccc cagtcggcga tgaattgtgc
     5761 ttttgcttcc gctaatgtca tgttgtaaag atagggccgg ttttcgaact ttcaaaatat
     5821 attgaaagtt ttatttcccg ccgcctcctc ggacaggcgc tggcctggct gtccgtttgg
     5881 ccgtttggcc gtttggccgt ttggtcgttt ggtcgtttgg cggcggctaa ttcttcggac
     5941 aggtatattc ctttgcctct ttgaaccgga accctttccc cgggcggatc atcagcgcca
     6001 ggtagaagcc cccgttctgc gtcttggttt tggagaagac attggcccag ttgtggaccc
     6061 ccagtaatag cggtcccgct tttaccgcgc cgccgaccca gacctttccg tccgtggtca
     6121 ccgtcaacgg caggtacccg ccgagattct ttttctccca gcgcggggta agggcgaaga
     6181 gggtgatctc ttttgtaaaa agccgttttg ccgtgttgtc gccgccaagg ttcagggtca
     6241 gttcggtgtt caaagcaaaa tgttcgggca gttgccggtc cacattgatc accagcctcg
     6301 ccgggttcca gatgatgaaa tgaccggtta aaggcgccat cgaattcacg atcgtagcaa
     6361 ggctgtcgtt gaattgggcc atggagccga cataattgaa tttctggttg agatcgacat
     6421 cggagacact gctcttggga ttgccggcgg tcctgctctg tgatccatac tggtagttat
     6481 tcccccccag atccagcagg gagacgccga tcttccattc atattcataa taatcgtctt
     6541 cgtcgtcgta t
//
LOCUS       DATGFG010000503         7799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_58740, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000503 DATGFG010000000
VERSION     DATGFG010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58740"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VKQ52_09315"
     CDS             complement(<1..668)
                     /locus_tag="VKQ52_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HLZ87428.1"
                     /translation="MKVVIIGGGIGGLAIGAFLYQKGVEIVINERSVHTPECGHAFLM
                     HRDGLSVLNELNAGHKATLPGETITRFTLKTPAGESIRETELDSWKCIKRGDLIAFLS
                     SLLPRDVVKAGRCFSHFLHEGDRIAAAVFLNGETEYGDLFIGSDGGNSRVRESLFGAV
                     RMTPVTVKEIVGIAASDHLAGRWPGMFMKFQHSGKGLAFGMIPTFKKEFVWFMQYDPS
                     LGDLA"
     gene            complement(665..1834)
                     /locus_tag="VKQ52_09320"
     CDS             complement(665..1834)
                     /locus_tag="VKQ52_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLZ87429.1"
                     /translation="MFDPTTIDLRILRERAYNLRWATVPEGVIPLTAADPDFPSAPEI
                     AEAICRFAKDRYLCYGPPEGLPEFKESMAGHYQAKRNLSIAPGNVFPVDSAAFGIYLT
                     CRAFLRPGDEAIIFDPVDFLFRYSIESVGGTAVAFPIPPGDAPVDFQRLENSITQRTR
                     LLCLCNPLNPTGKVFTKAELLCFGELACKYDLTILSDEIWSDIVYPPQIYTSIASLGE
                     EISARTVTVTGFSKSYGLAGLRIGAVMATDADRFKKLMDASLHHPTIHGANVLSQVAA
                     TAALKECGYWLTGFLTHLHAMRNLVVGALNSIPGFACHSPEGCYVAFADISRTGKTSA
                     AMRDLLFDKARVAIVPGLKEWFGEGAQGYIRISFATSEEIMQTALSNIKKTMQHL"
     gene            complement(1880..2269)
                     /locus_tag="VKQ52_09325"
     CDS             complement(1880..2269)
                     /locus_tag="VKQ52_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ87430.1"
                     /translation="MKNIFLIDDDPVFVFLTRKMIRSMNFKAEINEISDGLMAISYLS
                     ENIDKPELLPDIIFLDLSMPVMDGWEFLNEYALLQPRLTKKIALFIVSSSISPHEVER
                     SKTFQVVHDFLIKPLAKGKIREIIEEM"
     gene            complement(2274..3539)
                     /locus_tag="VKQ52_09330"
     CDS             complement(2274..3539)
                     /locus_tag="VKQ52_09330"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87431.1"
                     /translation="MDKETKSLSDRQAAGLAALAKQIVTTASLKAQVTAFKKNEEFSR
                     NNEADQKLVADKLDKQKKFYENILNKLPTDIAVFDPDHRYLFVNPGAISKEEYRKFII
                     GKDDYQYAEYRNRDISVAHSRRAKFLEVKNTGKEIRWEDSLPDPNGNIITHLRRLYPV
                     YDEAGALSMVIGFGIDITERKIMEEKQASLVKQLSAQNNQLVDFCNIVSHNLRAPLVN
                     ISMLAEFIEESEDADEQQLMISKLRPVVGNLHDTFNELVESIQIKQDLEIKTEDICLA
                     DCLQKTLDGLQTEINKLQAHIEVDFSKAPVIRYPPKYIRSILDNLVSNSLKYHSPARQ
                     PSIELRSEIVDKNIVLSIRDNGLGLDLTKHRDNVFKIGKVFHRHPNAKGFGLFMTKTQ
                     VESMGGRIWVESQPDKGTVFFIEFVHQKT"
     gene            complement(3644..3808)
                     /locus_tag="VKQ52_09335"
     CDS             complement(3644..3808)
                     /locus_tag="VKQ52_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87432.1"
                     /translation="MLPISRKPPSRRSRGRRARRQQQHQQQWHQVRRHQQQDQQQDQQ
                     DQQQQDQQRH"
     gene            complement(3812..3991)
                     /locus_tag="VKQ52_09340"
     CDS             complement(3812..3991)
                     /locus_tag="VKQ52_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87433.1"
                     /translation="MKTHSGGLRTLPGPLLPRNPGKPINPVNTVNPGSPGKPRKPGNL
                     ANTVNPAETKISKAL"
     gene            4318..5100
                     /locus_tag="VKQ52_09345"
     CDS             4318..5100
                     /locus_tag="VKQ52_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87434.1"
                     /translation="MAPKWIFSLEEYIKGPLGSFKALQVIIAAVCMLIPLTLKIMDRD
                     EFYPKQLTSAGLAVPGYSVRGFAVDSATSIRDNSGGFQLQVADSVLRIQIGTASLVPK
                     DKLGFRMSVSDYVYGSRSYVFGILYCLAAMLFVFNGVVYLHRRDTLLLRANGHWYNLW
                     IGLFLLGVALCPARVSPLWHVLFSVFFFAGNIGVLLFIANPGESRGSRMVRIGMAVAV
                     CLALILTWTGPLTLLWAEWISLVIIATHLILVTLSVDINPDP"
     gene            5120..6454
                     /locus_tag="VKQ52_09350"
     CDS             5120..6454
                     /locus_tag="VKQ52_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87435.1"
                     /translation="MHRLHGGFTVAATIKPGCATPLGDTLKRLNRTQGAGPADFGKAE
                     TVLFVSGVILPEDHYQGETLPETFVFATTYCGPLSVHLDDLVQTNAACLVAMFRHCEC
                     FPPGDSPDAKVIKDYLQSHSYPCTFSSRYQCMTKAEISREKRLREEIQKYLFQAQELN
                     AFDSLTALQVKTLIGRHIRIREDEFGWAFRPAKDMRFSEWLRFHRVAIITVLFIFLLD
                     FLFIKHESFIPVFLGKSLLWDAGSIAALAIVVLAIMWVISWSRNPTATRPADEAIRKI
                     AASQLHPVLNEMTAAAPLKSGNLRRWYYSSAHWVLRHFVKSIEVPTVSSIRWLVVNKR
                     RRLLFLSNFSNMTDFYVRDFLNGFWTPVGINFMFTNGRGFPDAALLFLNGIIGDPEGY
                     MNAVHAGQHVTDFWYAHEPLLTQDIIHKFRNIRNGLFREMNEAEAGEWLKLL"
     gene            6487..>7799
                     /locus_tag="VKQ52_09355"
     CDS             6487..>7799
                     /locus_tag="VKQ52_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87436.1"
                     /translation="MRAPYTIRPEVADYFSGDRKLAKDDMQGLIARGYDSLNASRYIL
                     LQIADADLAKKYYKDLADNYISRADKSNEPELTENRDPKRAVHLAFTHHGLKQLGLPE
                     AVRATFSRPFIEGMSYPFSDPDNPGNPGDPSGIISERSTVLGDTGRSAPANWYWGYGK
                     YYVDCVLLLYAENRAALDILLHEVYTGKATGLKLVRECEDFEYDENKLSREHFGFSDG
                     ISQPIIKGFSKSKVETSEEKLIEPGEFILGYKNEYGHYSPSPYITNPPPGTGLSLVPE
                     APEVPECKDRYDLGKNGTYLVFRQMEQHVEEFWRFHYHHSKEDAVSQTEKAVRLGAKM
                     IGRWPDGQPLVTCPSGVCGPDTGSLNEFMYYQQDKDGVNCPLGAHIRRTNPRDQVHTG
                     RNAAESLIMSRRHRMLRRGRIYGVPLVPDMDIEKMICLSKDKPSPA"
BASE COUNT         1646 a         2026 c         2235 g         1892 t
ORIGIN      
        1 gccaggtcgc caagcgaggg gtcgtattgc ataaaccaca cgaattcctt tttgaaggtg
       61 gggatcattc cgaacgccag ccccttcccg ctgtgctgga atttcataaa cattcccggc
      121 cacctgccgg cgaggtggtc actggcggcg atgccgacga tctcttttac ggtgaccggc
      181 gtcatccgga cagcaccgaa caacgattcc cgcacccgcg aatttccacc gtcggagccg
      241 atgaacagat cgccatattc tgtttccccg ttcaaaaaga cggcggcggc tatccggtcg
      301 ccttcatgga ggaaatgaga aaagcagcgg ccggccttca ccacgtcgcg gggaagcagg
      361 gaagacagga aagcgatcag atcgccccgt ttgatacatt tccaggaatc gagctccgtc
      421 tcccggatgg actcgcccgc aggtgttttc agggtgaagc gggtgatcgt ctcgcccggc
      481 agggtggcct tgtgcccggc gttcagttcg ttcagcaccg acaggccatc ccggtgcatc
      541 aggaaagcgt gaccgcattc aggtgtatgc accgatcgtt cgttgatgac gatctccact
      601 cccttctggt agaggaaagc gccgatcgcc agacctccga tcccacctcc gatgatgacg
      661 actttcatag atgttgcatt gtctttttaa tattggagag cgccgtttgc attatttcct
      721 ccgatgtggc gaaactgatc cggatatatc cctgcgcccc ctcgccgaac cattctttca
      781 gacccggcac gatcgccacc cttgccttat cgaataagag atcccgcatc gccgcgctgg
      841 tcttgccggt gcgggagata tcggcaaacg cgacataaca tccttcgggg gagtggcagg
      901 caaatccggg aatggaattc agggcaccga ccaccaggtt gcgcatggca tgcaagtgcg
      961 tgagaaatcc ggttagccag tatccgcatt ccttcagggc cgccgtagcc gccacctgtg
     1021 aaaggacatt cgctccatgg atggtcgggt gatggagcga ggcatccatc aattttttga
     1081 accggtctgc atcggtggcc atcacagcgc cgatgcgtag tccagcgagg ccataggatt
     1141 tgctgaaccc cgtgaccgtt accgttcttg cgcttatctc ctcgccaagg gaggcgatgc
     1201 tggtatatat ctgcggtggg taaacgatat cgctccatat ttcgtcggac aggatggtca
     1261 ggtcgtattt acaggctaat tccccgaaac aaagcagctc ggctttggtg aagactttgc
     1321 cggttgggtt cagcgggtta cagagacaga gcaggcgcgt ccgttgggtt atcgaatttt
     1381 cgagtcgttg aaagtccacc ggggcatcgc cgggagggat cgggaaggca acggccgttc
     1441 cgcccacgga ttcgatggaa taccggaaaa gaaaatccac cgggtcgaag atgatggctt
     1501 cgtcgccggg gcgcaaaaat gcccggcagg tgaggtagat gccgaaggcg gcgctgtcga
     1561 cggggaaaac attgcctggt gcgatagaaa ggtttctctt tgcctggtaa tggccggcca
     1621 tgctctcctt gaattccggc aggccctcgg gggggccata gcacaggtat ctgtctttgg
     1681 caaaacggca gatggcctcc gcgatctcgg gggcgctcgg aaagtcgggg tccgccgccg
     1741 tcaacgggat cactccttcg ggcacggtgg cccagcggag gttgtaggct ctttcgcgca
     1801 ggatacgcaa gtcgatggta gtagggtcga acatagggtc gggttaactg ggtccggtta
     1861 acaaggtcgg gttatatggc tacatctctt cgattatctc tcggatcttt cctttcgcca
     1921 gtggcttgat aagaaaatcg tgcaccacct ggaaggtctt cgatctttcc acttcatgcg
     1981 gcgagatgga agacgaaacg ataaaaagcg cgatcttctt cgtgagccgg ggctggagca
     2041 aagcgtattc gttcaggaac tcccagccgt ccattacggg catgctcagg tcaagaaaga
     2101 tgatatcggg taacagctcg ggcttgtcga tattttcgct caggtaactg atcgccatca
     2161 ggccatcgct gatctcattt atctcggcct tgaaattcat cgatcggatc atcttcctgg
     2221 tgaggaaaac aaatacggga tcgtcatcga taagaaagat atttttcatc ggatcaggtt
     2281 ttttggtgta cgaattcgat gaaaaaaacg gttcctttat cgggttggct ttctacccag
     2341 atccgtccac ccatggattc cacctgggtc ttggtcatga agagaccgaa acctttggca
     2401 ttgggatggc ggtggaatac tttgccgatc ttaaagacat tgtcccggtg tttggtcaga
     2461 tcgaggccca ggccattgtc ccggatggaa aggacgatat tcttatcgac gatctccgac
     2521 cgcagttcga tggacggctg tcttgcgggg gagtggtatt tcagggaatt gctgaccagg
     2581 ttgtccagga tgctccggat atatttgggc ggatagcgga tcacgggggc cttgctgaaa
     2641 tccacctcga tatgggcctg cagtttgttg atctcggttt gcaatccgtc gagggtcttt
     2701 tggagacaat ccgcgaggca gatgtcttcc gtctttatct cgaggtcttg ttttatctgg
     2761 atggattcga cgagttcatt aaaggtatcg tgcagattac cgaccacggg tcgcagcttg
     2821 gagatcatca actgctgttc atccgcatct tcgctttctt cgatgaattc cgccagcatc
     2881 gagatattca ccagcggggc gcgcaggtta tgggagacga tattgcagaa atcgaccagc
     2941 tggttattct gcgcggaaag ctgtttcacg agggaggcct gcttttcctc catgatcttt
     3001 cgctcggtga tatcgatgcc aaagccgatc accatcgaga gtgcgcccgc ttcgtcatag
     3061 acggggtata ggcgtcgcag gtgggtgatg atattgccgt tggggtccgg aaggctgtct
     3121 tcccaccgga tctctttccc cgtgttcttt acttcgagga actttgccct tctggagtgg
     3181 gcgacggaga tatcccggtt ccggtattcc gcgtactggt agtcgtcctt gccgatgatg
     3241 aacttccggt attcctcctt gctgatggcg ccggggttga caaacaggta gcggtggtcc
     3301 ggatcgaaga cggcgatgtc ggtaggcagt ttgttcagga tgttctcata gaacttcttt
     3361 tgtttatcca gtttgtcggc gaccagtttc tggtcggcct cgttgttccg ggaaaattct
     3421 tcgttctttt tgaaggccgt cacctgggct ttcagtgacg cggtcgttac gatctgtttc
     3481 gcaagggcgg cgaggccggc ggcctgtcgg tcggaaaggc tcttcgtctc tttgtccatg
     3541 acgcgtaaat gacccagcaa aacgccttcg gcggtggtga ggggaatgct ggcgacaagg
     3601 agcagggtgg tgtcggttgg tggtgatgct ggtggtgctg ctgctagtgc cgttgctggt
     3661 cctgctgctg ttggtcctgc tggtcctgct gctggtcctg ctgctggtgc cgccgcacct
     3721 ggtgccactg ctgttgatgc tgttgttgac gcctcgcccg acgaccacgc gatcgccgcg
     3781 aagggggttt ccgcgatatg ggaagcaagc gctataaggc tttcgaaatc ttcgtctccg
     3841 ccgggttgac cgtgttggcc aggttgcccg gtttgcgagg cttgcccggg ctgcccggat
     3901 tgactgtgtt gaccgggttg attggcttgc ccgggttgcg gggaagcaac gggcccggca
     3961 gggtcctcag cccgccggag tgtgttttca tatttatagg cgaattccat aatcttccct
     4021 ccaatgccgc tcttttttag cgtattggcc tccagattct atcgagaatt cgcataaatg
     4081 cgtggattac agagcaatgt ctgttctttc atagaatcgg atgccgttgc taaatagtta
     4141 acggattatt gagcggacta ttgtagtaaa aaggccagaa ctcgggccgg gatagcgctc
     4201 gggatcgggc ggggcgccta aaacaatcgt tggccgaccg ggggaaaaag ggtgatttca
     4261 cggtggttcg gtaagtggcg gcattgtatt tttaaggggt tggtaaatta caatgtcgtg
     4321 gcccctaaat ggatattttc gctggaagag tatataaagg gcccgctcgg ctcgtttaag
     4381 gccctccagg ttattatagc agccgtctgt atgctgatac ccctcaccct gaaaataatg
     4441 gaccgggatg aattctatcc gaagcagctg acttcggccg ggctggcggt gcccgggtat
     4501 tcggtccgtg gttttgcggt cgactccgcc acgtcgatca gggataacag cgggggattt
     4561 cagttacagg tagcggactc cgttctgcgg atccagatcg gtacggcatc cctcgtccca
     4621 aaggacaaat tgggtttccg gatgagcgtc agcgattatg tctacgggtc gaggagttat
     4681 gtatttggga ttctctactg tctggcggcg atgttgttcg tttttaacgg agtggtctac
     4741 ctccaccggc gggacacgtt gctcttgcgc gccaacgggc attggtacaa tctgtggatc
     4801 ggcctgttcc tgctgggcgt cgcgctctgc ccggcaaggg tcagtccgct ttggcatgtc
     4861 ctattttcgg ttttcttttt cgcgggaaat atcggcgtat tgctgttcat cgccaacccc
     4921 ggcgaatcga ggggaagcag gatggtcagg atcggaatgg cggttgccgt ctgcctggcg
     4981 ctgatactaa cgtggacggg ccctttaacg cttctatggg cggagtggat ttcacttgtc
     5041 attattgcga cgcatttgat cctggtgacc ctttccgtgg acatcaaccc cgatccttaa
     5101 ccaaaacctg ccgttcatta tgcaccgact acatggcgga ttcaccgtag cagcgactat
     5161 aaagccagga tgcgcgacgc ccttgggaga caccctgaaa cgtctgaacc ggacgcaggg
     5221 tgcggggcct gctgatttcg gcaaagccga aacagtgttg ttcgtctccg gcgtcatcct
     5281 gcccgaagac cattatcagg gagaaacctt gcccgagacc tttgtatttg ccaccaccta
     5341 ttgtggcccc ctttccgttc atctcgacga cctggtgcaa accaatgccg cctgcctggt
     5401 agccatgttc cggcattgcg aatgctttcc acccggggac agtccggacg ctaaagttat
     5461 aaaggactac ttacaatccc attcttatcc ctgcacgttt agctcccggt atcagtgcat
     5521 gacgaaagcc gagatctccc gggagaagcg gcttcgggag gagattcaaa agtatctttt
     5581 tcaggcgcag gaattgaatg ccttcgactc ccttaccgcg ttgcaggtta aaacgctgat
     5641 cgggcggcat atccgcatcc gcgaagacga gtttggctgg gctttccgtc ccgccaaaga
     5701 catgcgtttc tccgaatggc tgcgattcca ccgggtggcg atcattactg tcctttttat
     5761 ctttttgctg gactttctgt tcattaaaca tgagtccttc attccggtct ttttaggaaa
     5821 gagcctccta tgggatgcgg gatcgatcgc cgcattggct atcgttgtcc tggccattat
     5881 gtgggtgatc tcctggagca gaaaccctac ggccacccga ccggccgatg aggcgatcag
     5941 aaagatcgcg gccagccagc tccatcccgt gctaaatgaa atgacggccg ccgcaccctt
     6001 aaaaagcgga aacctgagac gatggtatta ctcttccgcc cattgggtcc tgcggcattt
     6061 cgtgaaatcg atagaagtac ccacggtcag ctccatccgc tggctggtcg tcaacaaaag
     6121 aaggcgactg ctttttcttt ccaatttttc gaatatgacc gatttctatg tacgggattt
     6181 tctgaatggc ttttggacgc ccgtcgggat caatttcatg tttaccaatg ggagggggtt
     6241 cccggatgct gccttgttat tcctaaatgg gatcatcggg gacccggagg ggtatatgaa
     6301 tgccgtccat gcagggcagc atgtaacgga cttctggtac gcgcatgaac ccctcctcac
     6361 ccaggatatc attcacaaat tcaggaatat caggaatgga ttgttcaggg agatgaatga
     6421 agcggaagcg ggtgaatggt tgaaacttct ataaaacaat ttgctggcaa tagtaaacct
     6481 tacatcatga gagcccccta taccatccga ccagaggttg ccgactattt ttccggcgac
     6541 cggaagctgg ccaaagacga catgcaggga ttgattgccc ggggatacga ctccctcaac
     6601 gcctcacggt acatcctgct ccagatcgcc gatgccgacc tggcaaaaaa gtattataaa
     6661 gacctcgccg ataattatat ctcgagggcc gacaaatcca acgaaccgga gctcaccgaa
     6721 aatcgtgatc ccaaaagggc ggttcacctg gcctttaccc atcatggcct gaagcagctt
     6781 ggattgccgg aagcagtacg agcaactttt tcaaggccgt tcattgaagg catgagctac
     6841 cctttttccg atccggacaa tccgggcaat ccgggcgatc catccgggat catcagcgag
     6901 cgatcgaccg tattgggaga taccggcaga agcgcccccg ccaactggta ttggggctat
     6961 ggcaaatact atgtggattg tgtactcctg ctatacgccg agaacagggc agccctggac
     7021 attttactac acgaggtata caccggtaaa gctaccgggt tgaaattggt acgcgagtgc
     7081 gaagattttg aatacgacga gaacaaactg tccagggaac attttgggtt tagcgacggg
     7141 atctcccaac cgatcatcaa gggcttctcc aaatcgaaag tggagacctc cgaagagaaa
     7201 ctgatcgagc ccggggagtt catattgggc tacaagaacg agtacgggca ctattctccc
     7261 agtccctata tcacgaatcc tcccccggga accggtcttt cgctggtgcc ggaggcgccg
     7321 gaggtgccgg agtgtaaaga caggtacgac ctgggcaaga acggaaccta tcttgtcttc
     7381 aggcaaatgg agcagcatgt ggaagagttc tggcgttttc actatcacca ctcgaaggaa
     7441 gatgccgtca gccagacgga aaaggcggtg cggctcggcg cgaagatgat tggccgttgg
     7501 cccgacggac agcccctggt cacctgcccg tccggggttt gtggtccgga taccggatcc
     7561 ctgaacgagt ttatgtatta tcaacaggat aaggacggcg tcaactgccc tttaggcgcg
     7621 catatcaggc gtaccaatcc ccgtgaccag gtgcacaccg gtcgtaatgc ggcggaatcc
     7681 ctgatcatgt cccgaagaca tcggatgctc cgccgcggcc ggatttacgg cgtcccgctg
     7741 gtccccgata tggatattga aaaaatgatt tgcctgtcga aagataagcc gtcgccggc
//
LOCUS       DATGFG010000504         4875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_133235, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000504 DATGFG010000000
VERSION     DATGFG010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133235"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VKQ52_09360"
     CDS             complement(<1..239)
                     /locus_tag="VKQ52_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87437.1"
                     /translation="MKKITPHLYQLDLGFVNAFLIEDIVPSPDPADDPAPSLPPTSGL
                     IPAPDLTPARAIAPGFTLVDTGTPGNLSKIFAQLK"
     gene            complement(276..851)
                     /locus_tag="VKQ52_09365"
     CDS             complement(276..851)
                     /locus_tag="VKQ52_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ87438.1"
                     /translation="MRIKDEQKIDLFLASTLTLVGQVGLVGLTMPLIAKRSGVAIGTL
                     YIYFKSKDDLIHALYKEVKNRFGATIFIGFSQELPVKEGFQVIWENSLRYVVSNYSEQ
                     VFLQQFIASPYKREDQAMAVARPVMAPLAQLFERGQKKGLLKPDTEFLMPSLLFGFVQ
                     QIAAHIHHKPARLTKAFMDTSFRFFWDAIRA"
     gene            complement(985..1215)
                     /locus_tag="VKQ52_09370"
     CDS             complement(985..1215)
                     /locus_tag="VKQ52_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87439.1"
                     /translation="MASPQEQKDKFEDGFQQIVDAAPVNVGSKWNGRYNWVDPPGKTA
                     SQPKIDTVVNKLPEELKEQYEDLLNNFRHNRL"
     gene            1379..2140
                     /locus_tag="VKQ52_09375"
     CDS             1379..2140
                     /locus_tag="VKQ52_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ87440.1"
                     /translation="MARIFITGSADGLGQLAAKTLVSQGHQVVLHARNPERGRQAMAK
                     TPGAEEVLTADLSSLRETVQLAKNVNELGAFDAVIHNAGVYQVSNPEQNIDGLPILLA
                     VNTIAPYILTCLIQKPKRLIYLSSGMHTQGDPSLKGLHGNTLRVTYSDSKLHDLMLSM
                     AVARKWKNVLSNAVDPGWVPTKMGGAGAPDNLQKGVETQVWLATSNDATAKVTGSYFH
                     HKKETRFLPEAANAAVQEKFLAICQQLSGVHFPGE"
     gene            complement(2227..2598)
                     /locus_tag="VKQ52_09380"
     CDS             complement(2227..2598)
                     /locus_tag="VKQ52_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87441.1"
                     /translation="MANNMTYEELVKKHPGSLVEKIVTEVVNKDTVEVHFEDEDDEQW
                     AVIKVHVYEEDKEMAIRLLSDDRWVLQFGYYDDEDEFIELLQPLTQPEIDLIPKGLKK
                     VMLKVLVSEQGLRLPGNFLSR"
     gene            complement(2692..3972)
                     /locus_tag="VKQ52_09385"
     CDS             complement(2692..3972)
                     /locus_tag="VKQ52_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HLZ87442.1"
                     /translation="MTNYRNFKIGCLVFLATTMFAPGCMNLFLSPSERSFQKGLQRFN
                     QKQYKEALDLFEESAAAGNGAAMNQIGWMNANGLGVPLDYGNALKWYKQAVRAGSTTA
                     LNNLGWMYQYGEGVQENKPRAVAFYQKAADAGNLTARCNLGSMYELGEGVPVDYSRAM
                     QEYGRAAALNHAAGMNHLGWMYEKGEGVAVDYQQALSWYHKAADRGNAYAINNLGMMY
                     QQGKGQPVDIKLAMENYQKAAKAGSPEAMNNIGWLYRNGLGVPENDSLAMLWYRRSAD
                     EGNDVGMFNIGWMYHHGAGVPVDYQQAIDWYQKAAKAKNSNAEGNMGWMYEKGLGVAR
                     DCRQAMDWYIRAAADGNTNAMDNLGWMYQHGDGVTRNVVLALEWYSKAADKGNRQAMT
                     NLAGMYEDGDGVPKDKGKAGEWREKAKQVSGAEE"
     gene            complement(4056..4520)
                     /locus_tag="VKQ52_09390"
     CDS             complement(4056..4520)
                     /locus_tag="VKQ52_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLZ87443.1"
                     /translation="MKKRDNYFVVMMVLTALIAGNPAAAQTSGPLYNEIAQLDSLQFD
                     AFNTRNLDHLMYFFDNSLELYQDNTGVRNYDQTKQAFGSLFKMDYVLTRNLVPGSMEV
                     YPIKDYGAIETGQHRFSHMENGQQQVSTYKFMQIWQKKEGVWRVTRGMTYGH"
     gene            complement(4537..>4875)
                     /locus_tag="VKQ52_09395"
     CDS             complement(4537..>4875)
                     /locus_tag="VKQ52_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87444.1"
                     /translation="ADYSRFLIWVMNGAGLSKALYQEMTSPLSKIKDNKYMGLGWAVY
                     PELPGGQYGLSHSGEDPGVKTLAIILPQSKRGLLIFTNSDNGPQLYTLLVKGYLKEQG
                     NAIFIIETKN"
BASE COUNT         1093 a         1248 c         1239 g         1295 t
ORIGIN      
        1 tttttcagtt gggcgaagat tttggagagg ttgccgggtg ttcctgtgtc gacgagggtg
       61 aagccgggtg cgatggcgcg tgctggggtg aggtcgggtg cgggaatgag gccggacgtt
      121 gggggcagac tgggtgcggg gtcgtcggcc ggatcaggag agggtacgat atcctcgatg
      181 aggaaggcgt tgacgaagcc gagatccagc tggtagagat gtggtgtgat ctttttcata
      241 aatgaatttt ttttcatttt tggtgcaaaa aaaaatcagg cccggatcgc atcccagaag
      301 aaccggaatg aggtatccat aaaggctttg gtgagtcgtg cgggtttgtg gtggatatgg
      361 gcggcaatct gttggacaaa gccaaagagc aggcttggca taaggaattc ggtatccggc
      421 ttcaggagtc cttttttttg cccgcgttcg aacagctggg cgaggggggc catcacgggc
      481 cttgccacgg ccatggcctg gtcttcgcgt ttgtagggag aggcaataaa ttgttgcagg
      541 aacacctgtt cggaatagtt ggagactaca tagcggaggc tgttctccca tatgacctga
      601 aaaccctctt tgacggggag ctcctggctg aagccgatga aaatagttgc gccaaagcga
      661 ttttttactt ccttgtaaag ggcatggata aggtcgtcct tgctcttaaa atagatgtac
      721 agggtcccga tggcaacgcc ggatcgtttt gcgatgaggg gcatggttag cccgacgagg
      781 ccgacctggc ctacgagtgt aagggtgctg gccaaaaaaa ggtcgatctt ttgctcgtct
      841 ttgatgcgca tggggcaaag gtagggaata atgaaaaaat attcattatt aattcgggac
      901 ccctaccggg tgataaatgg caggccgggt gagaaatggc gggccggtga tgaatggcct
      961 gccgggtgag aaatggcggc gtctctacaa tcgattatgc ctgaaattat tgagtaagtc
     1021 ttcatactgc tccttcaatt cctccgggag cttattgacc accgtatcga tcttgggttg
     1081 agacgccgtc tttcccgggg gatcaaccca gttgtaccgg ccattccatt tcgacccgac
     1141 gttgacgggt gcggcgtcta cgatctgctg gaacccgtct tcgaatttat ctttttgctc
     1201 ctgtggggat gccatacact tccgtttatt tcgcgggcaa ggtcggattt ttttggcatt
     1261 cgccggctat tatactcccc tgctggtgaa tggcaacggt tcctgccata gaggatataa
     1321 tacatagtga ggagaattcc agccaaatta gtaagttgca tagacatcat gataaaagat
     1381 ggcgagaata ttcattacag ggtcagcaga cgggctgggg caattagcgg ccaaaacatt
     1441 ggtcagccag gggcaccagg tagtattaca tgcccgcaat cctgaacgag ggcgtcaggc
     1501 catggcaaaa acacccggcg ccgaagaagt cctgaccgca gatttatcca gtctccggga
     1561 gaccgttcag ttagcgaaga atgtcaatga gctgggcgcc ttcgacgcgg ttattcataa
     1621 cgccggtgtt taccaggtct ccaaccccga acaaaatatc gacggattac ccatacttct
     1681 cgccgtgaat accatcgcac cctatattct cacatgcctg atccaaaaac ccaaacggtt
     1741 gatctatctc agttcgggga tgcatacgca aggtgatcca agcctaaaag gcctgcatgg
     1801 aaatacactg cgggtcacgt attccgattc caaactgcat gacctgatgc tttctatggc
     1861 ggtagcccgt aaatggaaaa atgtgttgag caatgcggtg gatcccggct gggtgccaac
     1921 aaaaatgggt ggcgccggcg cacccgacaa tctgcaaaaa ggcgtcgaaa cgcaggtctg
     1981 gctggcaacg agcaatgatg ccacagctaa agtcacgggt agttacttcc atcacaaaaa
     2041 ggagacccgt tttttgcccg aggcggccaa tgccgcggtg caggaaaaat tcctcgccat
     2101 ttgccaacaa ctcagcggcg tgcattttcc aggggagtaa catccatatt tacagggaat
     2161 ggcgctgggt ctttttgaga ttggagaaat gccaatttag actggtcttg catcgtgtcc
     2221 gtggcgctat ctcgatagga aatttcccgg gagccgcagg ccttgctcgc tgaccaggac
     2281 tttgagcatg acctttttaa gccccttggg aatcaggtct atttccggct gggtaagcgg
     2341 ctgaagcagt tcaatgaatt catcctcgtc atcatagtat ccaaactgca atacccatct
     2401 gtcgtcggac agtaaccgga tggccatttc cttgtcctct tcatagacat gcacttttat
     2461 tactgcccac tgttcgtcgt cctcatcctc aaagtgaacc tcgacggtgt ctttattcac
     2521 tacttctgtc acgatcttct ctaccagaga gcctgggtgc ttttttacca gttcttcgta
     2581 tgtcatattg tttgccatag atatcgcaaa gataatgcgc tatgtctagc caggactgac
     2641 gtcaatagaa aatatatttg aataaagcca accgggttgg tttcctatcc gtcattcctc
     2701 cgcccctgat acctgttttg ccttctcgcg ccattccccg gccttccctt tatcttttgg
     2761 aacgccgtct ccgtcctcgt acatgccggc gagattggtc atcgcctgcc tatttccctt
     2821 gtccgccgcc tttgagtacc attcaagggc tagcacgaca ttccttgtga ccccatcacc
     2881 atgctggtac atccatccga gattatccat cgcattggtg ttgccgtctg cggctgcccg
     2941 aatgtaccag tccatcgctt gcctgcagtc tcttgcaacg cccagtcctt tttcatacat
     3001 ccatcccatg ttgccttctg cgttgctgtt ttttgccttt gctgcctttt gataccagtc
     3061 gatcgcctgt tggtagtcca ccgggacgcc agcgccgtgg tgatacatcc agccaatatt
     3121 gaacatgcca acatcgttac cctcatcggc agaccgtcgg taccacagca tcgccaatga
     3181 gtcgttttcg ggcactccca agccgttccg gtacaaccag ccaatgttgt tcatggcctc
     3241 cggacttccg gcttttgctg ctttctggta attctccatt gccaatttaa tgtcgacagg
     3301 ctgcccttta ccctgctgat acatcatacc gaggttattg atagcgtagg cgttacccct
     3361 gtcagccgct ttatggtacc aggagagtgc ctgctggtaa tcgacggcga ctccttcgcc
     3421 cttttcgtac atccatccca ggtggttcat cccagccgca tgattcagtg cagccgcccg
     3481 tccgtactcc tgcatggccc gggaatagtc aacgggtaca ccctcgccca actcatacat
     3541 cgaacccaaa ttgcagcgag cagttaaatt cccggcgtct gctgcttttt gatagaaagc
     3601 cactgcccgg ggcttatttt cctgcacccc ttctccatat tgatacatcc accctaaatt
     3661 gtttaaagcc gttgtactgc cagctctcac cgcttgttta taccacttca gtgcgtttcc
     3721 gtagtcaagt ggaacaccca gcccatttgc attcatccac ccgatctggt tcatagctgc
     3781 gccgttgccg gcggcggctg attcctcgaa aaggtcgagt gcttccttgt attgtttctg
     3841 attaaatcgt tgcaggcctt tttgaaaaga cctttcactg ggactgagga agaggttcat
     3901 gcaccctggc gcgaacatgg tcgttgccag aaagaccagg catccaattt tgaaatttcg
     3961 ataatttgtc attcggtcaa aggagggttt ggctaacaag ataaggaatg taaggaacta
     4021 tattgcgttt tcatcccctg catctcaaag gcgaattagt ggccatacgt catccccctg
     4081 gtgacccgcc agacaccctc ctttttctgc catatctgca taaacttata ggtgctcacc
     4141 tgctgttgtc cgttctccat atgtgaaaac ctgtgctgcc cggtctcgat cgcgccataa
     4201 tcttttatcg gatacacttc catactgccc gggaccaggt tccgggttag cacatagtcc
     4261 attttaaaaa ggctgccgaa agcctgcttt gtctggtcgt agttacgtac gccggtgttg
     4321 tcctgataaa gctccaggct attatcgaaa aagtacatca ggtgatcgag gtttctggta
     4381 ttaaaggcgt cgaactggag agaatcgagc tgtgcgatct cgttatacag ggggcccgaa
     4441 gtctgcgccg cagcagggtt accggcaata agcgcggtca ataccatcat aactacaaaa
     4501 tagttgtccc gttttttcat ttgtattttt ttactgttag tttttcgtct caatgataaa
     4561 tatggcattc ccctgttcct tcagataacc cttgacgagg agagtataaa gctgcggtcc
     4621 gttgtccgaa ttggtaaaga tcagcagtcc gcgtttggat tggggtaata taatggcaag
     4681 cgttttaact ccagggtcct cgccgctgtg cgacaagccg tattggccac ccggcagttc
     4741 aggataaact gcccagccca ggcccatgta tttgttgtct ttgatcttag acaacgggct
     4801 ggtcatttcc tggtagaggg ctttggacaa accggcgccg ttcatcaccc aaatcaggaa
     4861 cctgctgtag tccgc
//
LOCUS       DATGFG010000505         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1237779, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000505 DATGFG010000000
VERSION     DATGFG010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237779"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="VKQ52_09400"
     CDS             complement(<1..1737)
                     /locus_tag="VKQ52_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLZ87445.1"
                     /translation="MINKRNLPIVLLVLAGGVFVAFRTLGLGGTPPTKYERILHSVGE
                     YLTQVHYSPKPIDDKFSEEVFHKYLKEVDGEKDVFLQSDVDNLGGRYSTKIDDEILGS
                     TTIQFVPAVTEVYRKRLAETEAIYKEVLSKPFDFTKDEDFNQNYEELKFPVNDADRKE
                     AWRKRLKYLTLERYAELLDQQEANKNKPGFVVRSNEELEKDARARSMKVMDRIYERLK
                     VKETDDDRFNMFVQCIVQSMDPHTDYFPPVEKRYFDEQMSGHFFGIGASLVYEDGNIK
                     IGSLVTGSPAWKSGQVGVGDIIQKVAQGSQEPVDMAGYFTEDAIKIIRGTKGTEVRLW
                     LKKPDGTVKMVSLIRDEIVQDELTFARSAIVNSPKGKIGYIYLPEFYADFDNPKGSRC
                     SIDVAKEIFKLKEQKVDGIVLDLRDNGGGSLYDVVQMAGYFVEQGPIVQVRDRDGKPS
                     IIPDHDKSVLYDGPFAVMVNELSASASEIFAAAIQDYHRGVIIGSTSTYGKGTVQRPY
                     GLDKTLGFMDANSELGTLKLTLQKFYRINGGSTQLRGVSSDIVLPDIYPDIYEYSKIR
                     EKDNPDALPWDEI"
     gene            1958..2031
                     /locus_tag="VKQ52_09405"
     tRNA            1958..2031
                     /locus_tag="VKQ52_09405"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1992..1994,aa:His,seq:gtg)
     gene            2085..2699
                     /locus_tag="VKQ52_09410"
     CDS             2085..2699
                     /locus_tag="VKQ52_09410"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HLZ87446.1"
                     /translation="MFHLSWWFYTIPFISAFIHWLTIWMALKLLFHPRHPKKILGFTL
                     HGVFPKRQKQIAESLGRIVGQELLSFGDIEQTITNPENVRRILPLAEEHIDNFLRVKL
                     KEQMPMISMFIGDKTIGQLKAVFMKELEDLFPVIMKNYVANLRHDLDLERIVVEKIAA
                     FSADRLENMLNEFLTKEFRFVEIIGAALGFLIGALQILLTLLMR"
     gene            2824..>3564
                     /locus_tag="VKQ52_09415"
     CDS             2824..>3564
                     /locus_tag="VKQ52_09415"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HLZ87447.1"
                     /translation="MRKTWLLFLALAMSAIGFSQGPPMGAGGGGRRGVGGQAPSIGHF
                     YGKVVDSKTGKGMDGASVQLIQSKFNPATRAQKDTVVGGMITGRHGDFSLENLPILGN
                     FRLRITAIGYGTYEQKVAFDLKGLRGAAGGGNGGGVGGGGTGDGQPNMAALQALNAID
                     KDLGNIKLAEDAQNLEQVTVTASKPLITMGVDRKIYNVEKDISAAGGSATDVMKNVPS
                     VQVDIDGNVTLRNSPPTIFVDGRPTTLTL"
BASE COUNT          820 a         1078 c          854 g          812 t
ORIGIN      
        1 gatctcatcc cagggcaggg catccgggtt atccttctcc cggatcttgc tgtactcata
       61 gatatcggga tagatatccg gtaaaacgat atcggaagac accccgcgca actgggtgct
      121 cccgccattg atccggtaga atttctgcag ggtcagcttc agggtgccca gttcgctgtt
      181 ggcatccata aagcccaacg tcttgtccag gccatagggc cgttgcaccg tccccttccc
      241 ataggtggag gtactgccga tgatcacccc gcggtgatag tcctggatcg ccgccgcgaa
      301 gatctccgac gcggaagcac tcagctcgtt gaccataacg gcaaaggggc cgtcatacaa
      361 aacactcttg tcatgatcgg gaatgatgga aggcttgcca tcccggtccc gcacctggac
      421 gatggggccc tgctcgacaa aataacccgc catctgcacc acgtcatata atgaaccgcc
      481 accgttatcc cgaagatcga ggacgatacc atccaccttc tgctccttca gcttgaagat
      541 ctccttcgcg acatcgatgc tgcaacggct gcctttcgga ttgtcgaagt cggcatagaa
      601 ctcgggcaga tagatatacc cgatctttcc cttgggcgaa ttgacgatcg cactgcgggc
      661 aaaggtcagc tcatcctgta cgatctcatc ccggataagc gataccatct tgaccgtacc
      721 atccggcttt ttcaaccaca accgcacctc cgtgccttta gtgccccgga tgatcttgat
      781 ggcatcctcg gtgaaataac cggccatatc caccggctcc tggctgccct gggccacctt
      841 ctggatgatg tcccccacgc ctacctggcc ggacttccac gcgggactgc ccgtcaccag
      901 cgaaccgatc ttgatattgc catcctcata cacgagcgag gcgccgatgc caaagaagtg
      961 gccgctcatc tgctcatcga aatatctttt ttccaccggg ggaaaataat ccgtatgcgg
     1021 gtccatcgac tggacgatac actgcacgaa catattgaac cggtcgtcat cggtctcctt
     1081 taccttcagc cgctcgtaga tgcgatccat caccttcatg ctccgcgcac gcgcatcctt
     1141 ctcgagctcc tcgttgctgc gcacaacaaa gccgggtttg ttcttattcg cctcctgctg
     1201 atccagcagc tcggcatacc gctcgagcgt gaggtacttc aaccgcttgc gccaggcttc
     1261 cttccggtcc gcatcgttga cgggaaattt caactcctca tagttctggt tgaaatcctc
     1321 gtcctttgta aaatcgaagg gcttggaaag gacctccttg tagatggcct ccgtttccgc
     1381 aagccgcttc cggtatactt ccgtcaccgc gggaacgaac tggatggtcg tactgccaag
     1441 gatctcgtcg tcgatcttgg tcgaataccg gcctcccagg ttatccacat cggactgcag
     1501 gaaaacatcc ttttccccat ccacctcctt taggtatttg tggaatactt cctcggagaa
     1561 tttatcgtcg atcggtttcg ggctgtagtg aacctgcgtg agatattcgc ctacactgtg
     1621 caggatgcgt tcatatttgg tgggtggggt acccccgagc cccagggtcc tgaaagccac
     1681 aaagacgccg cccgccaaaa ccaagaggac gatgggtaaa tttctcttat tgatcatatg
     1741 ctttagcact tttaacatag ctattcaaaa ataacgataa atataatggt atgttgtcgc
     1801 ccaagttgaa tattaacagc ctctttgcca catttacatg agcggcacgg gatacacggt
     1861 aaaatacccc gcgccgctca ttccatcccc aagacaaata attttacaca atgtttttgg
     1921 cgaaacccgt ttttgcatta attttgccgt tcaactggtg gttgtagctc agctggttag
     1981 agcatcagat tgtggttctg agggtcgtgg gttcgaaccc catcatccac ccgcaaaaaa
     2041 tagcaaaaag aggccatacc tggcctcttt tttataataa cactatgttc catttgtcct
     2101 ggtggttcta caccatcccc ttcatctccg cctttatcca ctggctcacg atctggatgg
     2161 ccctcaagct cctgttccac ccccgtcacc caaaaaagat actcggcttc actctccatg
     2221 gcgtcttccc gaaacgccag aaacagatcg ccgaaagcct cggccgcatc gtcggccagg
     2281 aactgctctc cttcggcgac atcgaacaga ccatcaccaa ccccgaaaac gtccggcgca
     2341 tcctgcccct ggcggaagaa catatcgaca acttcctccg ggtgaaacta aaagaacaaa
     2401 tgccgatgat ctcgatgttc atcggcgaca agaccatcgg tcagctgaaa gccgtcttta
     2461 tgaaggagct cgaagacctc ttccccgtca tcatgaagaa ctatgtcgcc aatctccgcc
     2521 acgacctcga cctcgaacgc atcgtcgtgg aaaagatagc cgccttctcc gccgatcgcc
     2581 tcgagaacat gcttaacgaa ttcctgacga aggagttccg cttcgtggag atcatcggcg
     2641 cggcccttgg ttttctcatc ggcgcgctcc agatcctgct cactttactg atgagataaa
     2701 ctgccttttt taagtgtatc gtattagccc ttccgcatct gattcctacc gtctgtatag
     2761 aaaccggcaa tttgctaaag gttttgacct gaattttgca ggcaaaatga atacttaata
     2821 ctcatgagaa aaacttggct tttgttcctc gcactggcaa tgtcggcgat cggcttttcc
     2881 cagggcccac ccatgggcgc cgggggcggc ggtagaagag gcgtgggcgg acaggctccc
     2941 tccatcggcc acttctatgg aaaagtagtg gactccaaga cgggaaaagg aatggacggc
     3001 gcctcggttc aactgatcca aagcaaattc aacccggcta cccgcgccca aaaagatacg
     3061 gtcgtcggcg gcatgatcac cggccgtcat ggcgacttca gcctcgagaa tctccccatc
     3121 ctgggcaatt tccggctcag gatcaccgcc atcggctatg gcacatacga acagaaagta
     3181 gcattcgatc tgaaagggct ccgcggcgcc gctggaggcg gcaatggcgg aggcgtgggt
     3241 ggcggaggga ctggcgacgg tcagcccaat atggccgccc ttcaggccct caacgccatc
     3301 gataaggacc tggggaatat caaactggcg gaagacgctc aaaacctcga acaggtgacc
     3361 gtaacggctt ccaagcccct catcacgatg ggcgtcgacc gcaaaatata caacgtggaa
     3421 aaagatatct ccgccgcggg cggctcggcc accgatgtca tgaagaacgt tccgtccgta
     3481 caggtggaca tcgatggcaa cgtcaccctt cgcaacagcc cgcccaccat cttcgtcgat
     3541 ggccgcccca ccacgctgac cctc
//
LOCUS       DATGFG010000506         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1063731, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000506 DATGFG010000000
VERSION     DATGFG010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063731"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VKQ52_09420"
     CDS             complement(<1..642)
                     /locus_tag="VKQ52_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HLZ87448.1"
                     /translation="MKPAIAVFWLRRDLRLEDNAGLYHALKSGIPVLPVFVFDRNILD
                     LLENKADRRVEFIYETLTAIRQQLRQLGSGLGVYYGRPPGMFRHLLAEYDVKAVYTNT
                     DYEPYAIDRDRDIGELLAEKNIPFHCYKDQVIFEKKEVLKDDGTPYTIFTPYSRRWKA
                     LLNDFYLSSYPVKKYYRHFLRKDPGPMPSLASMGFKKTGEPFPPAAVKTGLLRK"
     gene            complement(642..1490)
                     /locus_tag="VKQ52_09425"
     CDS             complement(642..1490)
                     /locus_tag="VKQ52_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HLZ87449.1"
                     /translation="MNPSADWINHNGVFLPAGTPVLTAGNRGFRYGDGLFETLLIKKG
                     QIRLRDYHFHRLFTGIDLLRFTKPALFTPASLEKQILRLCERNDHTALARVRLVVFRG
                     DGGLYDPQTLHPHYLIESWPLRSDDTGINENGLVIDIYPDGQKPCDPIANCKSNNFLL
                     YVLAALYAKDHRLNDCLVLNSHHRLADSTIANLFYVKQGQVFTPPLSEGCVAGVMRRF
                     LLATLPGAGFPVHERPVTAEDLLQADEVFLTNAIKGIRWVREFRTAQYASTLSGTIYR
                     QLIKEM"
     gene            complement(1505..2176)
                     /locus_tag="VKQ52_09430"
     CDS             complement(1505..2176)
                     /locus_tag="VKQ52_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87450.1"
                     /translation="MKRPELNQAIKVAIADDHALFRAGVKTALAVKKDVELVAEADNG
                     MQLLHLLKHVEPDVILLDIQMPIMDGIQTLPEIRKDHPHVKVIILSMHNDHSMISKLM
                     EIGANSYLTKNSDSETIYQAIKTCYEQEFFFNELTNKALLTGLRTKRPEIGAPQDVNL
                     TEKELTVLKLMCEEKTTKEIADIVDISPRTVEAIRDKLKTKTGAKSMAGLVMYAVKNG
                     IIQQQ"
     gene            complement(2876..>3494)
                     /locus_tag="VKQ52_09435"
     CDS             complement(2876..>3494)
                     /locus_tag="VKQ52_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87451.1"
                     /translation="HKIFRDGIKMALKNKEYLRILWEAEDGKDLVHKMQIKRPDVLLM
                     DIRMPEVDGINAIGLLRKEYEDVKIIVLTMYDDQEMISKMMEMGANAYLTKTTDPDEI
                     YQAILTCMNDDFYFNDLVNKAVLSKLQTKRQVRQFYPSPIKFSEKEIRILKLLAEDKT
                     TEEISKEVFLSTRTIETIRQNMKTKIGAKTIAGLIMYAMRNKIID"
BASE COUNT          818 a          826 c          903 g          947 t
ORIGIN      
        1 cttcctgagc aggccggttt ttacggcagc ggggggaaag ggctcgccgg tctttttgaa
       61 ccccatggag gcgagcgatg gcatcggccc gggatccttc cggagaaaat ggcggtaata
      121 tttttttacc gggtaggaag acaggtaaaa gtcgttcaac agggccttcc atctgcggct
      181 atagggagta aagatggtat agggggttcc gtcatccttc aggacctcct tcttttcaaa
      241 aatgacctga tccttatagc agtgaaaggg gatgttcttc tccgccaata gttccccgat
      301 gtcccggtcc ctatcgatgg catagggttc atagtctgta ttcgtataaa cggcctttac
      361 atcatattcg gccagcagat gccggaacat cccgggcggc cggccataat atacgcccag
      421 acccgacccc agttgccgga gttgttgccg gatggccgtc agggtttcgt agataaattc
      481 cacccgccgg tcggccttgt tctccaggag gtcgaggatg ttccggtcaa atacaaagac
      541 cggaagcacc ggaatgccgc ttttcagggc atgatagagc cccgcattat cctccagccg
      601 gagatcccgg cgcaaccaga aaacggcgat ggctggtttc actacatttc ttttatcagt
      661 tgtcgataga tagttccgga cagggtgctt gcgtattgtg ccgtacgaaa ttccctcacc
      721 cagcggatgc cttttatcgc gttggtgaga aagacctcat cggcttgcag cagatcttct
      781 gccgtgacgg gtcgttcatg gacgggaaag cccgccccgg ggagggtggc cagcaggaac
      841 cgccgcatga cgccggctac gcagccctcc gataacggtg gggtaaacac ctgcccctgt
      901 tttacgtaaa agaggttggc aatggtactg tccgcaagac gatggtggct attcagtacc
      961 aggcaatcat tcaaccggtg atcttttgca taaagggcgg cgagtacgta gagaaggaaa
     1021 ttattggatt tgcaattggc tattggatca cagggctttt ggccatccgg gtaaatatcg
     1081 atgacaagac cattctcatt tatgccggtg tcgtcggacc tcaggggcca ggattcgata
     1141 agataatgag gatgaagggt ttgaggatca tagagaccac catcaccccg aaagacgacg
     1201 agccttaccc gggcgagggc ggtatgatcg ttccgctcgc aaagccgcag gatctgcttt
     1261 tccagcgaag ccggggtgaa caaggcgggt ttggtaaacc ggaggaggtc gataccggtg
     1321 aagagacggt gaaaatggta atcccggagc cggatctgac ccttttttat cagcagggtc
     1381 tcaaaaagcc catccccata acggaatccc cggttacccg ccgtcagaac gggggttccg
     1441 gccgggagaa agactccatt atgatttatc caatcggcgg aggggttcac cggtcctatg
     1501 ggctttactg ctgttggatg ataccatttt ttacggcgta cataaccagg ccggccatgg
     1561 attttgcgcc tgttttggtc ttcagcttgt cccgaatggc ttcgacggta cgggggctga
     1621 tatccacgat atcggcaatt tccttggtgg tcttttcctc gcacatgagc ttcagaacgg
     1681 tgagttcctt ttcggtgaga ttcacgtcct ggggggctcc aatctcgggt cttttggtgc
     1741 gcaaaccagt tagtaatgcc ttgttggtca gctcattaaa gaagaattcc tgttcgtaac
     1801 aggtctttat agcctggtag atggtctcgg aatccgaatt cttggtgagg taggagtttg
     1861 cgccgatctc cattagcttg gagatcatcg agtggtcatt gtgcatggat aggataatga
     1921 ccttgacatg ggggtgatcc ttgcgaattt cgggcagggt ttggattccg tccattatag
     1981 gcatttggat atcaagcaga atgacatcgg gttcgacgtg ttttagcagg tgtaggagct
     2041 gcattccatt gtcggcttcc gccaccagtt ccacgtcttt ttttacggca agagccgttt
     2101 tgaccccggc cctgaaaagg gcatgatcat cggctatggc aactttaatt gcctggttaa
     2161 gctcgggtct tttcatacga tttagaattt aggaacacaa aattctgtct ttgcggacaa
     2221 gccattttac cggctccggg cagttggacg gactgccgaa tatacttgca aattgaagca
     2281 aaaaatgcaa tacttactat aggtaaacta cgtaacaact tgttttcgga atatacgatc
     2341 cctacgaaga taagtggttt tttcccgata ttaaagagga atttatacga atccggacat
     2401 cgttgatcta cgaaagggcc agtaaaagcc gaaaagtcaa aatagggcaa agttgcgctt
     2461 gcctacctgt ccggccaccg ctatttttgt acaagttgat tgacaggatt agcaacctcg
     2521 aaccgtccaa tgtcctagaa accgtccaga accttttttc aatattctat gagacccggc
     2581 aaaatccaat agtcaatcaa ctttattccg caagtagttc gatatcctga taaagcgaaa
     2641 aaccagtatc aagtatccaa tccagatcca ttgagaagcc gatccaggtt cctatgaaaa
     2701 acaagcctga tcatttccaa tatcccattt ccgcaattca tcgcgccgtt gctgtatcag
     2761 gtgactaggt atcctctctg gtttgtaaag tttttgcagg ttttctatgc atcgggcata
     2821 gagaaccttt tttgttcgta tcgacgatgg gtattcgttg gtatgggaag ggagctcagt
     2881 cgatgatctt attccgcatg gcgtacatga taagaccggc aatcgttttg gcgccgatct
     2941 ttgttttcat gttctggcga atggtttcga tcgtgcgggt actgaggaag acttccttgg
     3001 agatctcttc ggtcgtctta tcttctgcga ggagtttcag gatgcggatc tccttttcgg
     3061 aaaattttat gggacttggg tagaattgcc ttacctgtcg tttggtctgc agtttggaca
     3121 gaacggcctt attcaccagg tcgttgaaat aaaagtcatc attcatgcaa gtaagaatag
     3181 cctggtatat ttcatcaggg tcggtggtct ttgtcagata ggcattggcg cccatctcca
     3241 tcattttgga gatcatttcc tggtcgtcat acatggtcag cacgatgatc ttgacatcct
     3301 catattcctt ccgcagcagc cctattgcgt tgatgccgtc gacttccggc atccggatat
     3361 ccatcagcaa aacgtccggt cttttgatct gcatcttatg caccaggtct tttccgtctt
     3421 cagcctccca taaaatcctg agatattcct tgttcttcag agccatttta ataccgtcgc
     3481 ggaaaatctt atga
//
LOCUS       DATGFG010000507         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1610681, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000507 DATGFG010000000
VERSION     DATGFG010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610681"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VKQ52_09440"
     CDS             complement(<1..665)
                     /locus_tag="VKQ52_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ87452.1"
                     /translation="MRKLYYGLLAVVLPAGFLGAQTLTRDEAVRIALKNNLGIELARN
                     NVAIAGVYNSYGIAGGLPVVTGSVSDQEQSTSLKQEYANPANNKSSNNAFSNNLSGSV
                     NGYMLLYNGERVVTAKKRLGTIETQTRQQLSSRALILVNNVLLKYYDVVRQQSYARTL
                     QASIDAQKQQLAIVQAQQSVGLANNADLFQSQVDLNTQVQNLQAQQLVIDQDKTDLLT
                     LLTL"
     gene            complement(685..3750)
                     /locus_tag="VKQ52_09445"
     CDS             complement(685..3750)
                     /locus_tag="VKQ52_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ87453.1"
                     /translation="MNISELSLKRPVLATVMNLMLILFGVVGYNFLAVRDYPAIDPPI
                     ITVQTNYTGANPDLIESQITEPLEKNINGIPGIRTISSTSSLGSSYITVEFNLGVDLE
                     AAASDVRDKVGQAQRSLPLDIDAPPVVTKSDANADFILILAVQSRTKSLMELSDYAEN
                     VLQQQLQTIDQVSSVNVFGDKTYSMRIWLDQDKMNAYSIAYNDIATAMNNENVELPPG
                     KIYGNNTELTIKTLGRLTTEKDFRDVILREDSSGIVRLSDVARVELGPQSPEQSWKYN
                     GVNAVGLAIIPQPGANNIAIADEFNRRLAEIEKSNKSDLVFNVLIDNTQTIRRSLAEV
                     KETLLIAFALVVLVIFFFFRNWLIALRPLIDIPISLIATFFIMYVAGFTINILTLLGI
                     VLATGLVVDDGIVVTENIFRKLEQGFPIRKAALEGSREIFFAVISTSLTLAVVFMPVV
                     FLQGFVGRLFREFGVVLAAAVLISSFVSLTITPVLNVYLTSKRSGHGWFYDKTEPFFT
                     GMENGYKRLLMGFMRIRRMAFVLIAACGGLIWLILGNLQSEIAPLEDRSSIRFMVTLP
                     EGASYSYTQNVTDQVASFLYDSVPERDFVFARTPAGSNTNSAQPRIGLTEPQQRGRSQ
                     NQIAADLQRKVARFNDARIFAIQEQTIAVGSGSKTSLPVQFVLENQDLDKMKKILPAF
                     LEACRKDKTFSNVDVNLKFNKPELQITLDRMKIKDLGLSTNDVVSAMQAAFSGGRLAY
                     FIMNGYQYYVIAQVERKDRNDPTDISKIYVRNKSGNPIPLDAVVHIEQNSSPSTLYHF
                     NRYKAATISASLADGKTIGDGIVAMQAIANRMLDASFQTALSGPSRDYAESSSNIVFA
                     FVLALVLIYLVLAAQFESFVDPFVIMLTVPLALAGALLSLWVFHQTLNIFSEIGMIML
                     IGLVTKNGILIVEFTNQKREQGMGKMEAVVEAASQRLRPILMTSLATSLGALPIAMSL
                     GAAATSRIPLGIVVVGGILFSLILTLFVIPAVYTFISSKRKVHLTEVTE"
BASE COUNT          863 a         1207 c         1003 g          803 t
ORIGIN      
        1 agggtcagca aggtcagcag atcggtcttg tcctggtcga tgaccagttg ctgcgcctgc
       61 aaattctgta cctgggtatt cagatccacc tgcgactgga acagatcggc attgttggct
      121 aaccccacac tctgctgcgc ctgcacgatc gccagttgct gcttctgtgc atcgatcgac
      181 gcctgcagcg tccgggcata actttgctgc cgcactacat cataatattt cagcaacaca
      241 ttgttcacca ggatcagcgc ccgcgacgac aactgctgcc gggtctgcgt ctcgatggtg
      301 cccagccgct ttttagccgt caccacccgc tccccgttgt ataataacat atacccgttg
      361 accgaaccgg acaggttgtt ggagaaggca ttgttgctgg acttattgtt ggccggattg
      421 gcatactcct gcttgagact ggtcgactgt tcctggtcgg acacactgcc cgtcaccaca
      481 ggcagcccgc cggcgatccc atagctgttg taaacgccgg caatggcgac attattcctc
      541 gccagctcga tcccaagatt gttctttaac gcgatgcgta ccgcctcatc ccgcgtcagc
      601 gtctgcgccc ccaaaaaacc ggccggcaac accaccgcca gcaatccata atatagtttt
      661 ctcataatca tctggccaaa agaattattc ggtcacttcc gtcaagtgta ccttccgctt
      721 actcgaaata aaggtataca ccgccgggat cacaaacagg gtcaggatca gcgagaacag
      781 gatcccgccg acaaccacga tccccagcgg gatccggctc gtggccgccg ccccaagact
      841 catcgcgatc ggcagggccc caagggaagt ggccagtgaa gtcatcagga tcggacgcag
      901 ccgctgcgaa gccgcttcca ccaccgcctc catcttgccc atcccctgct cccgcttctg
      961 attcgtgaat tccacgatca ggataccgtt cttcgtcacc agcccgatca gcatgatcat
     1021 cccgatctcc gaaaagatgt tcagcgtctg gtggaacacc cacaggctca gcaaggcccc
     1081 ggcgagcgcc agcggcacgg taagcatgat cacaaacggg tccacaaagc tctcgaactg
     1141 cgccgccagc acaagataga tcagcaccag cgccagcacg aaggcaaaga cgatattcga
     1201 ggagctctcc gcatagtccc gtgaaggccc cgacaacgca gtctggaagg aggcatccag
     1261 catcctgttc gcaatggcct gcatggccac gatcccgtca ccgatggtct ttccgtcggc
     1321 aagggatgcc gaaatggtag ccgccttgta ccggttgaag tgatacagcg tcgaaggact
     1381 gctgttctgc tcgatatgga cgacggcatc cagcggtatc gggttcccgc tcttgttccg
     1441 cacatagatc ttgctgatat cggtggggtc gttacggtcc tttctttcca cctgcgcgat
     1501 gacataatac tgatagccgt tcatgatgaa ataggccagc cggccgccgc taaaggctgc
     1561 ctgcatcgcg gaaacgacat cgtttgtcga caagccgaga tccttgatct tcatcctgtc
     1621 gagcgtgatc tgcaattccg gtttattgaa cttcaggttg acatccacgt tggaaaaggt
     1681 cttatccttc cggcaggcct cgagaaaagc cggcaggatc ttcttcatct tgtccaggtc
     1741 ctggttctcc agcacgaact gcaccggcag actcgtcttc gaccccgatc ccacggcaat
     1801 cgtctgctcc tggatggcga agatgcgcgc atcgttgaac cgcgccacct tgcgctggag
     1861 atcggcggcg atctggttct gactccgccc ccgctgttgc ggttcggtca atccgatacg
     1921 cggctgcgcg gaattggtat tactccccgc cggcgtccgc gcaaagacaa aatcccgctc
     1981 cggaacggag tcatagagga aactggccac ctggtcggtc acattctgcg tataggaata
     2041 actcgcccct tccggcaggg tcaccataaa gcggatactg ctgcgatctt ccagcggcgc
     2101 gatctcgctt tgcagattcc ccaggatcag ccagatcagc ccgccgcaag cggcgatcag
     2161 tacaaaggcc atccggcgaa tccgcataaa gcccattagc agccgcttat aaccattctc
     2221 catccccgta aagaaaggtt ccgtcttatc atagaaccag ccatgccccg accgcttgct
     2281 ggtcaggtag acattcagca ccggcgtaat ggtcagcgag acaaaggaag agatcagcac
     2341 ggccgcggcc agcaccaccc cgaactcccg gaacagccgg cccacaaatc cctgcaaaaa
     2401 gaccacgggc ataaagacca ccgccaaggt caaagacgtg gagatcaccg caaagaagat
     2461 ctccctgctg ccctccagcg cagccttgcg gatgggaaac ccctgctcca gttttctaaa
     2521 aatattttcc gtgacgacaa tcccgtcatc gacgacaagc ccggtggcca gcacgatacc
     2581 aagcagggtc aggatattga tcgtaaaccc ggccacatac atgataaaga aggtggcgat
     2641 aagcgaaatg gggatatcga tcagcggccg cagcgcgatc agccagttgc ggaagaaaaa
     2701 gaagatcacc agcaccacca gcgcaaaggc gatgagcagc gtctccttca cctcggcaag
     2761 cgaacgccgt atcgtctggg tattatcgat cagcacattg aacaccaggt cggacttgtt
     2821 ggacttctcg atctccgcca gccgccggtt gaactcatcg gcaatggcga tattgttggc
     2881 gccgggctgt gggataatgg ccagccccac cgcattcacg ccgttgtatt tccagctctg
     2941 ctccgggctc tgcggcccca gctccacccg cgcgacatcg ctgagccgga caatacccga
     3001 actgtcctcc cgcaggatca catcccggaa atccttctcc gtcgtcagcc ggcccagggt
     3061 cttgatcgtc agttccgtat tgttcccata gatcttcccc ggcggcagct ccacattctc
     3121 attgttcatc gccgtggcga tatcgttata cgcgatgcta taggcattca tcttgtcctg
     3181 gtcgagccag atccgcatgg aataggtctt gtcgccgaat acattcaccg aactcacctg
     3241 gtcgatggtc tgcaactgct gctgcagcac attctccgca taatcgctca gctccatcag
     3301 gctcttggtc cggctctgca ccgcgaggat aagaatgaaa tcggcattgg catcggactt
     3361 ggtcacgaca ggcggcgcat cgatgtcgag cggcagcgac cgctgcgcct gccccacctt
     3421 atcccgcacg tcactggccg cggcctccag gtcgacgcca aggttgaatt cgacggtgat
     3481 atagctgctg cccagcgaac tcgtggaaga gatcgtacgg atccccggga tgccgttgat
     3541 attcttctcc agcggctcgg tgatctggct ttctatcaga tcggggttgg cacccgtata
     3601 gttcgtttgc accgtgatga tcggcggatc gatggcaggg taatcccgga cggcaagaaa
     3661 attatagccc acaacgccga acaggatgag catcaaattc atcacagtgg ccagtaccgg
     3721 tcgcttgaga gataactctg aaatgttcat agcgggattt atttggcgtt ggcagccgtt
     3781 ggagtgactt cttcttccgt ccggacactc cggatcgtga ccagcgcatc gggccgcgca
     3841 aaaagtacac ccgtcacgac gacggtatcc ccggga
//
LOCUS       DATGFG010000508         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_326163, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000508 DATGFG010000000
VERSION     DATGFG010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326163"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VKQ52_09450"
     CDS             complement(<1..889)
                     /locus_tag="VKQ52_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87454.1"
                     /translation="MNEQIAGSTPNDLISLEISKPVGPRELVFRYLKYLPWIAICVIL
                     GFVLGYIKIRYYVPIYMVQSSLLIKNDEQAGGGKDDRLGALFLADPGTNLANEIQLLR
                     STPVISRVVRNLNLQTWYYNNGKLRSSLLYKNAPFHLLILHAADSTAAVTVTVTVLND
                     REFTLDGDVKTRYAFNRPFAIRNNQLMLIRDPAASFAPYQRPVFTASWQSATDVAGGL
                     ASAIKIVPGTDNSTILQLSMETEDVELGKDVLNALMSVYDSLIVEDKNRIAVNTMQFI
                     DKRLDTLRDQLGGIEGNLRS"
     gene            complement(902..1729)
                     /locus_tag="VKQ52_09455"
     CDS             complement(902..1729)
                     /locus_tag="VKQ52_09455"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HLZ87455.1"
                     /translation="MHQPTAVWKILLLNGLVLAFFSTMLCSCGNTRHLVYMQGQFDTA
                     KLSQTLPADPVIQKGDILSIIVYSDNPEATKIYNQTLITTATAGGGVSAGASEGLGAT
                     PSSPGYLVDENGNIEFQGLGLLHVAGLTRTVLKDSLNFRLLPFLQNPYYTIRFLNYKF
                     TMLGEVGKPGIYSIPGDHINIFEALGLAGDMTFYGRRDNVLVIRESDGKRQFARLDLT
                     KPEIMASPYFFLQQNDVVIFEPSKKKIAANDQVTARNISLAATLISVLAVLYGIFHH"
     gene            complement(1733..2263)
                     /locus_tag="VKQ52_09460"
     CDS             complement(1733..2263)
                     /locus_tag="VKQ52_09460"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HLZ87456.1"
                     /translation="MSVSANNWYVVYTKPRWEKKVYQLFSERKIEAYCPLNRVRKKWS
                     DRMKWVEEPLFKSYVFVHVKEEEQTAVRMVPGVINFIYWLGKPAIVKHSEIETIRKFL
                     NDYEEVTAEPITLEKNMKVTIRKGAFMDKEAKVVKVLNNKVKVVIESIGYALVAVIDR
                     SNVLQAENARSKRQSI"
     gene            2735..>2925
                     /locus_tag="VKQ52_09465"
     CDS             2735..>2925
                     /locus_tag="VKQ52_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87457.1"
                     /translation="MEPPSWSGRLSRLRQLFWLFFLLTIAYMIWVRTYLSPLSSDEIV
                     QFEIAKTVSKAQAIIADWK"
BASE COUNT          697 a          792 c          703 g          733 t
ORIGIN      
        1 agctccgcag gttgccctcg atgccgccca actggtcccg gagcgtatcg aggcgcttat
       61 cgatgaattg catcgtgttc accgcgatcc ggttcttatc ctcgacgatc agcgagtcgt
      121 acaccgacat cagggcattg agcacatcct tccccagttc cacgtcctcg gtctccatgg
      181 acaactgcag gatagtggaa ttatccgttc cggggacgat ctttatcgcg ctggcgaggc
      241 ccccggcaac atcggtcgcc gactgccagc tggcggtgaa caccggccgc tggtagggtg
      301 caaagctcgc ggcagggtcc cggatcagca tgagctggtt attgcggatg gcaaacggcc
      361 ggttgaaggc ataacgggtc tttacatccc catcgagcgt gaactcccgg tcgttgagga
      421 ccgtcacggt gaccgtcacc gccgcggtgg aatcggcggc atgcaggatc agcagatgga
      481 acggcgcatt cttatacagc agactggacc gcagtttacc gttattgtaa taccaggttt
      541 gcaggttgag gtttcttacc acccgcgaga tcaccggcgt actccgcaac agctggatct
      601 cgttggcgag attggtgccg gggtcggcca ggaagagcgc ccccaaccgg tcatccttgc
      661 cgccgcccgc ctgctcgtca ttcttgatca acagggagga ctgtaccata tagatcggta
      721 catagtaccg gatcttgata taccccagga caaagcccag tatcacacag atggctatcc
      781 agggaagata tttcagataa cgaaacacca gttccctggg gcctaccggc ttgctgatct
      841 ccaggctgat gaggtcgttc ggggtggaac ctgctatttg ttcattcatg gatggacact
      901 gttaatgatg gaaaatacca tagagaaccg cgagtacgga gatgagtgtt gcggcaaggg
      961 atatgttgcg ggccgtaacc tggtcgttcg cggcgatctt tttcttcgac ggctcgaaga
     1021 tcaccacgtc attttgctgc aggaaaaaat agggggaggc catgatctcc ggcttggtaa
     1081 gatcgagccg cgcgaactgg cgcttcccat ccgattcccg gatgaccagc acattgtccc
     1141 gccggccgta aaaggtcata tccccggcga gaccaagcgc ctcgaagata ttgatatgat
     1201 ctccgggaat ggaatagatc cccggctttc ccacctctcc cagcatcgta aacttataat
     1261 tcagaaaacg gatcgtataa tagggattct gcagaaaggg caataaccgg aaattcaggg
     1321 agtccttcaa caccgtgcgg gtcagccctg ccacatgaag caggcccagc ccctggaatt
     1381 cgatattgcc gttctcatcg acaagatagc ccggtgagct tggcgtggcg cccaatcctt
     1441 cggaagcccc cgcactgacc ccgccgccgg ctgtggcagt cgtgatcagc gtttggttat
     1501 agatcttggt ggcttccggg ttgtcactat acacgatgat actcaggatg tcccctttct
     1561 ggatgaccgg gtcggccggc aaggtctgac tcagcttcgc cgtatcgaat tgcccctgca
     1621 tgtagaccag gtgccgggtg ttcccgcagg agcaaagcat tgttgaaaaa aatgccagta
     1681 caagcccgtt caggagtaat attttccaca ctgccgttgg ttgatgcata tatcagatgg
     1741 attgtctttt tgatcttgca ttttctgcct gcaatacgtt cgaccggtcg atcacggcaa
     1801 ccagtgcata accgatactc tctatcacca cttttacctt attgttgagc accttcacca
     1861 ccttcgcctc cttgtccatg aacgccccct tccggatggt cactttcata ttcttttcga
     1921 gcgtgatcgg ctcggcggtc acttcctcat agtcattgag gaatttccgg atggtctcga
     1981 tctcgctgtg tttcacgatg gccggcttcc ccagccagta gatgaaattg atcaccccgg
     2041 gcaccatcct gacggccgtc tgctcctcct ccttcacatg aacgaacaca taggacttga
     2101 agaggggctc ttccacccac ttcatccggt cgctccattt cttccgcacc cggttcaacg
     2161 ggcaatacgc ctcgatcttt ctttcggaga aaagttgata cacttttttc tcccatcgcg
     2221 gtttggtgta aacgacatac cagttgtttg cagacactga catagcttcg cctcctcagt
     2281 cacagacatt gatttgtaac taattactta tcagccactt gtcctggaag ttcaattgct
     2341 tccaacggaa aagggcataa gcgagcattt tgttaaaaat tactccttat acccttccat
     2401 tctattgtaa aactaaaact cggaatgtat tcttcacagg actaaaaaaa tatttttacg
     2461 cgttgcgatc aaaaaaacaa acaaagccgg gatttattgc tttcaaagga tacaaattct
     2521 tataaaagaa gctcttacat ttcttaggca caaatataag gtattttaat attcaatatc
     2581 ttcaattgtt aattgaaaaa caatgtttta atcgtcaaaa caaatttcat gatcccttaa
     2641 tccggagccc gcaataaaag aaaaaagtta tcaccacagg cccatgatcg cgccggattt
     2701 tctaacttaa cggccacaag aaatcggtat ttgtatggaa cctccttcgt ggtccggccg
     2761 tctgagccgt ctgcgacaac ttttctggct cttcttcctc cttaccatcg cttacatgat
     2821 ctgggtaaga acgtatcttt ctccgctaag ctcagacgag atcgtacagt tcgagatcgc
     2881 aaagacggtt agcaaagctc aggccataat tgcagactgg aagat
//
LOCUS       DATGFG010000509         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_66914, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000509 DATGFG010000000
VERSION     DATGFG010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66914"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..776
                     /locus_tag="VKQ52_09470"
     CDS             66..776
                     /locus_tag="VKQ52_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87458.1"
                     /translation="MYSKSMDGRNILIVEDEKKIADTLKMGLAENGFDVEVAYDGKIG
                     YQLLMSRNFDLVILDINLPGMNGYELCKAIRNRDMNMLVIMLTSMSSLDDKIEGYDAG
                     ADDYLVKPFEFRELLLKIRALLKRTPGQAVPVGNLLKAADLEMNLDNKEVHRAGKRIK
                     LTAKEFQLLEYLMRNKNRVVSRADLAINVWDVDFNTNTNVIDVYISYVRNKVDRDFEH
                     KLIQTHVGMGYILKTQPN"
     gene            776..2167
                     /locus_tag="VKQ52_09475"
     CDS             776..2167
                     /locus_tag="VKQ52_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ87459.1"
                     /translation="MAVRTRITLLFTLLVVAILTLVCASVYYFSYTNRIKDIQTRLSN
                     RAITTGRLLSQGGVFDQALIRKIDASTSMAMKDKVVEAYDRSDHRIYWYSDNPADTLR
                     TDRNILDKARKSKDRIYFTQGIKDAIAFYYADENASFIIIAAAYDEAGNEKLRHLRLV
                     LSLSFIGGILISVIGGYLFSISLLHPIKRIADEVNDISARNLTRRIRSATGDPRDEWY
                     YLSDTLNQLLNRLQESFEIQGRFIANASHELSTPLTSISSQLEVALQRMRTMEEYRKV
                     IQSVYQDTRQLSKLTQTLLEFARASGTAGGLEIDLIRVDEILLRLPAELTKANPLFTV
                     TLDFDKLPEAEEELLVFGNEDLLFTAIRNIVTNACKYSDDHHATVRLTIQAKEIAIAV
                     EDKGRGIGEEEWEKIFQPFYRSDDGLAVPGFGLGLSLSRRILQLHKGSVRLHSVVGKG
                     STFAITLPPAAKI"
     gene            2245..>2943
                     /locus_tag="VKQ52_09480"
     CDS             2245..>2943
                     /locus_tag="VKQ52_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ87460.1"
                     /translation="MRIKHIALAGLIPLMLFSCVSSKKFKGLQSKYDSLNSAYSSLQN
                     DLKNCNGKTAGLTRDKSDCDARVAALQKQVDFLKENNTQALKQLEDMSVISSAQAQSI
                     RKSLDNLGAKDAYIQNLQAAMAHKDSLNMALVSNLKGAIGNLNDQDINIKVDKGVVYV
                     DISDKLLFATGKYNVTPRAGEVLGKVAQVLKAQPDIEFMVEGHTDNVPFRRGDLLDNW
                     DLSVKRATAVIRILQ"
BASE COUNT          742 a          946 c          686 g          569 t
ORIGIN      
        1 gttagagccg cattagaatt ggattagaga gtcccggact cctgccaaat ccttaattta
       61 gcagggtgta tagtaaatcc atggacggcc ggaatatact catcgtagaa gacgaaaaaa
      121 agatcgcgga caccctgaaa atggggctgg ccgagaacgg atttgacgtc gaggtcgcct
      181 atgacggcaa aataggctac caactcctga tgtcccgcaa cttcgatctc gtcatcctgg
      241 atatcaacct gcccgggatg aatggctacg aactctgcaa agccatccgc aatcgcgaca
      301 tgaacatgct cgtcatcatg ctcacgtcca tgagctcgct cgacgacaag atcgagggat
      361 acgacgccgg cgccgacgac tacctcgtca aacccttcga attccgcgaa ctcctgctga
      421 agataagggc cctgttgaaa cgtaccccgg gacaggccgt acccgtaggc aacctcctga
      481 aagccgccga tctggaaatg aacctggata ataaggaagt gcatcgcgcg ggtaaacgga
      541 taaaactcac ggcaaaggaa tttcaattgc tggaatacct catgcgcaac aagaatcgcg
      601 tcgtctcccg cgccgatctc gccatcaacg tctgggacgt ggatttcaac acaaatacaa
      661 atgtcatcga tgtatacatc agctatgttc gtaacaaggt ggacagggat ttcgaacaca
      721 aactcattca aacccatgtc ggcatgggct acatcctgaa aacccagccc aactgatggc
      781 ggtgcgcacc cgcataacgc ttctctttac cctcctcgtg gtggccatcc ttactctcgt
      841 ctgcgcatcg gtctactact tttcttacac caaccgcata aaggatatcc agacacgcct
      901 ctccaatcgc gccatcacca ccggccgcct cctgagccag ggcggcgtct tcgatcaggc
      961 cctcatccgc aagatcgacg cctccacctc catggcgatg aaagataagg tagtggaagc
     1021 ctatgaccgg tccgaccatc gtatctactg gtacagcgac aaccccgccg acacccttcg
     1081 cacagaccgg aacatcctcg acaaggcccg gaaaagcaag gaccggatct acttcaccca
     1141 gggaataaag gacgccatcg ccttttatta tgccgacgag aatgcttcct ttatcattat
     1201 tgccgcggcc tatgacgagg ccggcaacga aaaactccgc cacctccgcc tcgtcctttc
     1261 cctgagcttt atcggcggca tactcatctc cgtcatcggc ggctatctct tttccatcag
     1321 cctcctccac ccgataaaac gcatcgccga cgaggtgaac gacatctcgg cccgaaacct
     1381 tacccgccgc atccggtccg ccacgggcga tcccagagac gaatggtatt acctctccga
     1441 tacgctgaac cagttgctca accgcctgca ggagagcttc gagatccagg gccgctttat
     1501 cgccaacgcc tcccacgaac tctcgactcc cctgacctcc atctccagtc agctggaagt
     1561 agccctgcaa cggatgcgga ccatggagga ataccggaag gtgatccagt cggtgtacca
     1621 ggacacccgg cagctgagca aactcaccca aaccctgctg gaatttgccc gggcctcggg
     1681 cacggccggc ggactggaga tcgacctcat ccgcgtcgat gagatcctcc tgcggctccc
     1741 cgcggaactc acaaaggcaa accccctctt caccgttacc ctcgacttcg acaaactgcc
     1801 cgaagccgag gaagagctgc tggtcttcgg caatgaagac ctgctcttca ccgccatccg
     1861 gaatatcgtc accaatgcct gcaaatactc cgacgaccac cacgccaccg tccggctgac
     1921 catccaggca aaagagatcg ccatcgccgt ggaagacaag ggccggggga taggggagga
     1981 ggaatgggag aagatcttcc aacccttcta ccgcagcgac gacggcctcg ccgtgcccgg
     2041 ttttggcctc ggcctctcct tgtcccgccg gatactccaa ctccataaag gctcggtccg
     2101 cctccattcc gtcgtcggta aaggctccac ctttgcgatc accctccccc cggccgcaaa
     2161 aatctaatca aattctaatc cgcctttaag ccggcactaa tttcttcacc caaccttgta
     2221 gggtaaatca atcaaacaat atttatgcgt atcaagcaca tcgcactggc tggtctcatc
     2281 ccgcttatgc tcttctcctg cgtcagcagc aaaaaattca aagggttgca gtcaaaatac
     2341 gatagcctca acagcgccta cagctccctg caaaacgatc tgaagaactg caacggcaag
     2401 accgccggac tcacccgtga taagtccgat tgtgacgccc gtgtcgccgc cttgcaaaaa
     2461 caggtcgatt tcctgaagga aaacaatacc caggccctga aacagctcga agacatgtcc
     2521 gtcatcagca gcgcccaggc ccagagcatc cgcaagtcgc ttgacaacct cggcgcaaag
     2581 gatgcctaca tccagaacct gcaggccgcc atggcgcata aggattccct caatatggcc
     2641 ctcgtgagca acctcaaagg cgccatcggc aacctgaacg atcaggacat caatataaag
     2701 gtggataaag gcgtcgtcta tgtcgacatc tccgacaaat tgctcttcgc aaccggtaaa
     2761 tacaacgtca caccccgcgc cggggaagtg ttgggtaagg tagcccaggt gctcaaggcc
     2821 caacccgata tcgaattcat ggtcgaaggc catacggata atgtcccctt tcgccgcggc
     2881 gatctcctcg acaactggga tcttagcgta aaacgcgcca ctgccgtcat ccgcatcctc
     2941 cag
//
LOCUS       DATGFG010000510         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1464185, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000510 DATGFG010000000
VERSION     DATGFG010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464185"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VKQ52_09485"
     CDS             complement(<1..692)
                     /locus_tag="VKQ52_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLZ87461.1"
                     /translation="MADLKFQRNFGIAAHIDAGKTTTTERILRYTGMIHKIGEVHDGA
                     ATTDWMEQEKERGITITSAAVSCQWNFPTDKGKVDANTKKYYFNIIDTPGHVDFTVEV
                     ERSMRVLDGLIALFSAVDGVEPQSETVWRQANRYRVPRIGFVNKMDRSGADFLMVVNQ
                     VREMLGAKAVPLQLPIGAEDDFKGVVDLIKMKGIIWHMETEGMTFDEIPVPDDMMADV
                     LEWRAKLVEAVA"
     gene            complement(814..1281)
                     /gene="rpsG"
                     /locus_tag="VKQ52_09490"
     CDS             complement(814..1281)
                     /gene="rpsG"
                     /locus_tag="VKQ52_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HLZ87462.1"
                     /translation="MRKTQAKKLPLAPDARYNDILVTRFVNNLMWQGKKNTAFTIFYD
                     ALEKVAKTTNEDGYDIWKRALSNITPAVEVRSRRIGGATFQIPSEVRPDRKISLSMKW
                     LIRYSRERNGRSMAEKLAGEIVAASKGEGGAFKKKEDTHRMAEANKAFAHFKV"
     gene            complement(1317..1730)
                     /gene="rpsL"
                     /locus_tag="VKQ52_09495"
     CDS             complement(1317..1730)
                     /gene="rpsL"
                     /locus_tag="VKQ52_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HLZ87463.1"
                     /translation="MPTIQQLVRKGREIIRAKSKSRALDACPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNKVEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGSL
                     DTAGVKDRKKSRSKYGTKKEKAGAKGAAAKPAGKK"
     gene            complement(1797..>2561)
                     /gene="ilvE"
                     /locus_tag="VKQ52_09500"
     CDS             complement(1797..>2561)
                     /gene="ilvE"
                     /locus_tag="VKQ52_09500"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR01123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HLZ87464.1"
                     /translation="AERMMMPAVPEEIFIEGMRRLIDLDRDWIPMKSDYSLYIRPFMF
                     ATDDAIGVKASDTYKFMILLSPTGPYYSAPMRIYVEEKYTRAARGGFGFAKAAGNYGG
                     SMLATAVARQRGYDQVLWTDAFEHKYVQEIGTMNVFFVIGDKVITPDLEAGTILEGVT
                     RDSAMELLRDEGYTVEEGPVNIDDLVRAYTAGELKEVFGTGTAATISMIKELAYEDIT
                     MKFDTDKWVVAPTVKRVLTDIREGRREDTRGWMFRV"
BASE COUNT          568 a          794 c          558 g          641 t
ORIGIN      
        1 tcggcaacag cctccaccag tttggcgcgc cattcgagaa catcggccat catatcgtca
       61 ggcaccggaa tctcgtcaaa ggtcatccct tccgtttcca tatgccagat gatccccttc
      121 atcttgatca ggtcgaccac gcccttgaaa tcatcttcgg cgccgatcgg caactgcaga
      181 gggacagcct tcgcaccgag catctcccga acctggttga caaccatcag gaagtcggca
      241 ccgctgcggt ccatcttgtt cacaaacccg atccggggta cccggtaacg gttggcctga
      301 cgccaaacag tctcggactg gggctcaacg ccgtcgacgg cggagaacag ggcgatcagc
      361 ccgtccagca cccgcatcga acgctccacc tcaacggtga aatccacgtg acccggagta
      421 tcgataatat tgaaatagta ttttttggta ttggcatcca ccttcccctt gtcggtcggg
      481 aagttccatt ggcagctgac agcagccgag gtgatcgtga taccgcgctc tttttcctgc
      541 tccatccagt cggtggtagc tgcaccatca tgcacctccc cgatcttgtg aatcatcccc
      601 gtgtagcgca aaatccgctc cgtggtagtg gttttaccgg catcgatatg agccgcaatc
      661 ccgaagtttc tttgaaattt taagtctgcc atattgttta ttgaaaagtt gcgcaaaggt
      721 acgaaaaaaa attcatcata ccccttctaa aaacaaaaac cccgcggcat ctcttcagat
      781 tcgcggggtt tttatatata atttagtact tatctatact ttaaagtgag cgaaagcttt
      841 attagcttca gccatccggt gcgtatcttc ctttttcttg aatgcaccac cctcaccctt
      901 gctggccgca acgatctccc cggcaagctt ttcagccatg ctccggccat tcctttcacg
      961 gctgtaacgg ataagccatt tcatgctcaa agagatcttc cggtccggac gaacttcgga
     1021 agggatctgg aacgtagccc caccgatacg ccggctgcga acctcgacag cgggagtgat
     1081 gttggaaaga gcccttttcc agatatcata accatcttca ttggtcgtct tggccacctt
     1141 ttccagggca tcatagaaga tagtgaacgc ggtattcttc ttaccctgcc acatcaggtt
     1201 attcacaaaa cgggtcacca ggatgtcatt gtaccttgcg tcgggggcca gcggtaattt
     1261 tttggcttgc gtcttcctca ttgtttggcg gaattatact tagttataat attttattat
     1321 ttctttccag cgggtttagc tgcggcgccc tttgcaccgg ccttctcctt cttcgtacca
     1381 tacttgctcc ggctcttctt ccggtccttc acccctgccg tatccaggct gccgcgaacg
     1441 atgtggtaac gtacccccgg aaggtccttc acacgaccac cacggatcag cacgatcgag
     1501 tgctcctgga gattgtgccc ttcacccggg atatacgcga tcacctccac cttgttcgtc
     1561 aaccgcacct tcgctacctt acgcagggcg gagttgggct tcttgggagt ggtcgtgtaa
     1621 acacgggtgc aaacaccacg gcgctgcgga catgcatcca aagccctgga cttgctcttt
     1681 gcacggataa tctctcttcc ttttctaact aattgctgta tggtaggcat tctgaaccta
     1741 ttttattttg ggactgcaaa agtacagtcc cgcgtcttat aatccaaatt tttctgctat
     1801 actctgaaca tccatccccg ggtatcctcc cggcggcctt cccggatatc ggtcaacacc
     1861 cgcttcaccg taggggcaac aacccattta tccgtgtcga atttcatcgt aatatcctca
     1921 taagcaagct ctttgatcat cgaaatcgtc gccgcggtac ccgtcccgaa cacctccttc
     1981 aattccccag cggtatacgc ccggacgaga tcatcgatat tcaccgggcc ttcttccacc
     2041 gtatacccct catcccgcaa taactccatg gcactgtccc gcgtaacccc ttcaaggatc
     2101 gtccccgctt ccaggtccgg ggtgatgacc ttgtccccga tgacaaaaaa gacgttcatc
     2161 gtcccgatct cctgtacata tttatgctcg aaggcatccg tccacaacac ctggtcatac
     2221 ccccgctgcc tggccaccgc agtcgccagc atgctcccgc catagttacc cgccgccttg
     2281 gcaaacccaa aaccgccccg ggccgcccgc gtatatttct cctcgacata gatccgcatc
     2341 ggcgcggagt aatacggacc cgtaggacta agtaatatca tgaacttata cgtatccgaa
     2401 gccttcaccc cgatcgcatc atccgtcgca aacataaagg gccggatata aagcgaataa
     2461 tcactcttca tcgggatcca atcccggtcg agatcgatca gccggcgcat cccttcgata
     2521 aagatctcct ccggaacagc aggcatcatc atccgctcgg c
//
LOCUS       DATGFG010000511         5901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_697053, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000511 DATGFG010000000
VERSION     DATGFG010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697053"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VKQ52_09505"
     CDS             <1..1060
                     /locus_tag="VKQ52_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLZ87465.1"
                     /translation="SAFNFPVAVWSWNSMIAWVCGNTCVWKPSEKTPLCALACQHIVS
                     AVFAANNVPEGVCGLVTGGRELGEWLAADTRIPLISATGSTRMGKAVGTTVAARLGRS
                     LLELGGNNAIIVSADADLNIAIPGAVFGAVGTAGQRCTTTRRLIIQEKVYDSVKEKLT
                     AAYGQLRIGDPLDEKYHVGPLIDTDAVKAYQQAVAAAKQQGCRFLVEAETLAGDGFES
                     GCYVRPCIAEADPQYPIVAQETFAPILYLLKYKTLEEAIAIQNGVPQGLSSSIMTLNL
                     REAEQFLSAAGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTN
                     TINYSSQLPLAQGIKFNL"
     gene            1159..2619
                     /locus_tag="VKQ52_09510"
     CDS             1159..2619
                     /locus_tag="VKQ52_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ87466.1"
                     /translation="MKKWASLLLLAITSYCSVHAQVRLGILGGLHSSKVLETNHIPGW
                     DTTTKPFNSTRSGFQLGLILEMPIGHHGLFFQPAFTYITKGRTYDKNNDSVKSLLTDT
                     IYNKQTLKLSYMEIPLNFTYKLPLTANRMNSFFISAGPYVSFVYSGNVTTESLTATTK
                     KYSTETDPVTVGKGPDTYSTLDYGVNARAGFEIGNVMLSAYYSRGLGNFYHASYSGTF
                     HHELVGATLGIWLTSSGPAKALRKKDTDRDGVNDDVDLCPLQPGPAAWHGCPVPDTDH
                     DGVDDDHDSCRTIPGVGRYNGCPVPDTDHDGIDDEHDSCRTVPGLARYNGCPIPDRDH
                     DGINDEEDKCPDSAGTAENHGCPVVVVPEIKKEETEQINFIAHNVNFASASDRLLDSS
                     YVALDQLAGLLLAHPAWHLTIEGYTDNSGTPAKNLLLSRKRADAVKVYLVHKGIPATR
                     LTPAGFGQEKPIADNRTARGKAANRRVELKLSIMPE"
     gene            2754..4466
                     /locus_tag="VKQ52_09515"
     CDS             2754..4466
                     /locus_tag="VKQ52_09515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HLZ87467.1"
                     /translation="MIGSLRSLNGWLLGILLGCSPFTMFAQGTSVKSELPKGWHLLDK
                     ERDGYYGISVNKAYEFARLKKLKSKTILVAVIDSGVDTLHEDLKGILWTNPKEIPGNG
                     IDDDHNGYVDDIHGWNFIGGKDGTNVKDDSQEEARVYYTFKEKFEAPGLDTAALSPED
                     KDNYRMWAKARKTIMGDGTESGVDLVILHKALTACVKSDSILQQAMGKVEYTGNDLDT
                     FQTRTSESKSAKAVFLYLFKANKIMDMTNKAFLSDFSEQVDQEERKASAKENPPKDYR
                     HDIVKDDEKDFNDRYYGNNDVMAGDPMHGTHVTGIIAGEKLNGKGQIGIADNVRVMMI
                     RAVPNGDEHDKDIALAIRYAVDNGARVINMSFGKNLSPEKPWVDSAVKYAESKGVLLI
                     HAAGNDNADVDTTDNFPNPNFRHSTYVASNWITVGASSDPLAEPGFQSYTASFSNYGK
                     QEVDVFAPGTRIYSTLPGGRNYGNLQGTSMAAPVVTGVAALLLEYYPNLTPQQVKYCI
                     EKSAVAPPTKVKVPGSEDTMVNLSDISRSGGIINAYEALKLASTLNTAPEKPKKSTLH
                     NAMN"
     gene            4525..5034
                     /locus_tag="VKQ52_09520"
     CDS             4525..5034
                     /locus_tag="VKQ52_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ87468.1"
                     /translation="MIPRTEIIPNDMYNGYIALIKENDFREAIKKNTRQFRKTVENIP
                     RKKFDHAYAEGKWTIRQMLQHIIDAERVFAYRALRLSRKDPTPLPGFDENLWAVNDGG
                     SARRWKDLLEEFKAVRESTEYLYDALSDDQLRFTGTVRELPLNAFTIGYILPGHVTHH
                     LRILEERYL"
     gene            5060..>5901
                     /locus_tag="VKQ52_09525"
     CDS             5060..>5901
                     /locus_tag="VKQ52_09525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ87469.1"
                     /translation="MNLLLSLPPRRANRIAVSCFFFLAGLCFSSWASRIPSIQQKLHL
                     NNVSLGGVLLALPCGLMASLPLAGWLVAKFGSRPIAIGAGLLYASILPLLGFAGAVWQ
                     LVTVLFFFGMAGNMLNIAINTQAIGTESLYGRTIMASYHGLWSLAGFSGASIGGLFLG
                     LGWLPWQHFLVITGAAWLLICFMARNLVPRDLAADENRPIFARPDRSLINLGIIAFCS
                     MICEGTMFDWSNVYFQKVIFPPKALAGIGLTAFMSTMATGRFVGDWVTTRLGIKRILQ
                     VSGIL"
BASE COUNT         1362 a         2012 c         1452 g         1075 t
ORIGIN      
        1 ctccgccttt aacttcccgg ttgccgtatg gagctggaac agtatgatcg cctgggtttg
       61 cggcaacacc tgcgtctgga aaccctccga aaagacccca ctctgcgcgc tcgcctgtca
      121 gcatatcgtg tccgccgtct ttgccgccaa taatgtcccc gagggggtct gcggcctcgt
      181 caccggcgga cgggaactcg gcgaatggct ggccgcggat acccgtatac ccctcatctc
      241 cgccaccggc tccacccgca tgggcaaggc cgtaggcaca accgtggccg cccgcctggg
      301 cagatccctg ctggaactgg ggggcaacaa tgccatcatc gtctcggcag acgccgacct
      361 caacattgcc atccccgggg ccgttttcgg cgccgtcggc acggccggcc agcgctgcac
      421 caccacccgc cgcctcatca tccaggaaaa agtatacgat agcgtaaagg agaaactcac
      481 ggccgcctat ggccagttac gcatcggcga ccccctcgac gagaagtacc atgtcggtcc
      541 cctcatcgac accgacgccg taaaagccta tcaacaggcc gtcgcggcgg ccaaacaaca
      601 gggctgccgc ttcctcgtcg aagccgagac cctcgccggc gacggcttcg aaagcggttg
      661 ctatgtccgg ccctgcatcg ccgaggccga tccccaatac cccatcgtcg cccaggagac
      721 cttcgccccc atcctctatc tgctgaaata taagaccctc gaggaggcta tcgccatcca
      781 gaacggcgta ccccagggcc tctcctcctc catcatgacc ctgaacctcc gcgaagcgga
      841 acaattcctc tcggccgccg gcagcgactg cggcatcgcc aacgtcaaca tcggcaccag
      901 cggcgccgag atcggcggcg ccttcggcgg ggaaaaagag accggcggcg gccgcgaaag
      961 cggaagcgat gcatggaaag cctatatgcg ccgccagacc aataccatta actactcatc
     1021 ccaattgcct ctggctcaag gaataaaatt caatctctaa gacccctgcc cgaccttgtc
     1081 gctgcaacgc atttttcgcc aaccggcccc ggcgcaaaaa taattggtgg gattacgcgt
     1141 ttactatcat ctgcagttat gaaaaaatgg gcatcgcttc ttttattggc catcaccagc
     1201 tactgttccg ttcacgcaca ggtcagactg ggtatcctgg gtggcctgca ttcttccaag
     1261 gtattagaaa ccaatcatat acccggatgg gacacgacca cgaaaccctt taacagcacc
     1321 cgctccggtt ttcagctcgg cctgatcctg gaaatgccca tcggtcatca tggcctcttc
     1381 ttccagcccg ccttcaccta tatcaccaag ggccgcacct atgacaagaa caacgattcc
     1441 gtcaaaagcc tcctcacgga taccatctac aacaaacaaa cactgaagct ctcctacatg
     1501 gagatcccgc tgaatttcac ctataagctg ccgctcaccg ctaaccgcat gaacagtttc
     1561 ttcatcagcg ccggccctta cgtcagtttt gtctacagcg gcaacgtcac caccgaaagc
     1621 ctcaccgcca ccacaaagaa atacagcacc gagaccgacc ccgtcaccgt gggaaaaggc
     1681 cccgacacct attcgaccct ggattacggc gtcaacgccc gggccggctt cgagatcggc
     1741 aatgtcatgc tgagcgctta ttatagtcgc ggcctcggca acttctatca tgcctcctat
     1801 tccggaacct tccatcatga actggtgggc gccaccctcg gtatctggct cacctcctcc
     1861 ggtccggcaa aggccctccg gaagaaggat accgaccgcg acggggtcaa cgacgacgta
     1921 gacctctgcc ccctccagcc cggacccgcc gcctggcatg gctgccccgt cccggacacc
     1981 gaccacgacg gcgtggacga cgaccacgat tcctgccgca ccattcccgg cgtcggccgc
     2041 tacaacggct gccccgtccc cgataccgac cacgacggca tcgacgatga acacgattcc
     2101 tgccgcaccg tccccggcct ggcccgctac aacggctgcc cgatacccga ccgcgaccat
     2161 gacggcatca acgacgaaga agataaatgc cccgacagcg ccggaacagc ggagaaccat
     2221 ggctgccccg tcgtagtcgt ccccgaaata aagaaagaag agaccgaaca gatcaatttc
     2281 atcgcccata acgtcaattt cgcctcggcc agcgacaggc ttctcgacag ctcctacgtg
     2341 gccctggatc aattggccgg cctcctgctg gcccaccccg cctggcatct gaccatcgaa
     2401 gggtataccg ataattccgg taccccggcc aaaaacctcc tcctgtcccg caaacgggcg
     2461 gacgcggtca aggtctacct ggtccacaag ggcatccccg cgacccggct gacaccggcc
     2521 ggctttggcc aggaaaaacc catcgcggat aaccgcaccg ccaggggcaa ggccgccaac
     2581 cggcgtgtgg aactaaaact cagtattatg cccgaatagg ccttccgggc gcctcagaca
     2641 ggaaatttaa catgttaata aggccattcc tgtaattttt gccgggggtt ggcgcgagtt
     2701 ttatttattt tacaggattc aacacgacta cacataaact aaaaacgaca aaaatgattg
     2761 gaagcctgcg aagcttgaat ggatggttat tagggatact gcttggttgc tcgccgttta
     2821 cgatgtttgc ccaggggaca agtgtaaaat ccgaactgcc gaagggctgg catctgctgg
     2881 acaaggaaag ggacggatac tatggtatca gcgtcaataa agcctacgaa tttgcaagat
     2941 tgaagaaatt gaaaagcaag accatcctcg tggcggtgat cgactcgggg gtggataccc
     3001 ttcacgaaga cctcaaaggc atcctctgga ccaacccgaa agagatcccc ggcaacggca
     3061 tcgacgatga ccacaacggc tatgtcgacg atatccacgg ctggaatttc atcggcggca
     3121 aggatggcac caatgtaaaa gacgactccc aggaagaagc ccgggtatac tacaccttca
     3181 aggagaaatt cgaagcgccg ggcctcgaca ccgccgccct cagtcccgaa gacaaggaca
     3241 actaccgcat gtgggcaaaa gcccgcaaga ccatcatggg cgatgggacg gaaagcggcg
     3301 tcgacctcgt catcctccac aaggccctca ccgcctgcgt caagagcgat agcatcctcc
     3361 agcaggccat ggggaaggtg gagtacaccg gtaacgacct ggacaccttc cagacccgca
     3421 cgtcggagag caagagcgcc aaggccgtct tcctctatct cttcaaggcc aataagatca
     3481 tggatatgac aaacaaagcc ttcctctccg acttctccga acaggtggac caggaggaaa
     3541 gaaaggcctc cgcaaaagag aacccgccaa aagattaccg ccacgatatc gtaaaggatg
     3601 acgaaaaaga tttcaacgat cgctattacg ggaacaacga cgtcatggcc ggtgacccga
     3661 tgcacgggac acacgtgacg ggcatcatcg ccggggaaaa actcaacggc aaaggccaga
     3721 tcggtatcgc cgacaatgtc cgcgtgatga tgatccgcgc cgtgccgaac ggggacgaac
     3781 acgataagga catcgccctc gcgatccgct atgccgtcga caacggcgcc cgcgtcatca
     3841 acatgagctt tggcaagaac ctctccccgg aaaaaccctg ggtggattcg gccgtaaaat
     3901 acgccgaaag caagggggtc ctcctcatcc acgcggcggg taacgacaac gccgacgtag
     3961 acacaacgga caatttccct aaccccaact tccggcactc gacctatgtc gcctccaact
     4021 ggatcaccgt aggcgccagc agcgaccccc tggccgaacc cgggttccag agctataccg
     4081 cctccttctc caattacggt aaacaggaag tcgatgtgtt cgcccccggc acccgcatct
     4141 actctaccct gcccggcggc aggaattatg gcaacctcca gggcaccagc atggccgcgc
     4201 ccgtcgtcac cggcgtcgcc gccctcctcc tggaatacta tcccaacctt accccgcaac
     4261 aggtaaaata ttgcatcgag aagtctgccg tggccccgcc taccaaggtg aaagttccgg
     4321 gctccgaaga cacgatggtc aatctctcgg atatctcgag atccggcggt atcatcaacg
     4381 cctacgaagc gctaaagctc gcctcgacgc tgaacaccgc accggaaaaa ccaaaaaagt
     4441 ccaccctcca caacgcaatg aactaaaaaa aaggccgggt ttatcccggc ttctttttta
     4501 tatttagtat acaaacaccg acacatgatc ccacggaccg aaattatccc taacgacatg
     4561 tacaatggct atatcgccct gataaaggag aatgacttcc gcgaagccat aaagaagaac
     4621 acccggcaat tccgcaaaac ggtagagaac atcccccgca agaaattcga tcatgcctac
     4681 gcggaaggta aatggaccat ccgccagatg ctccagcata tcatcgacgc cgaacgcgtt
     4741 ttcgcctatc gcgccctgcg cctgtcccgt aaagacccga ccccgctgcc cggcttcgac
     4801 gagaatctct gggccgtcaa tgacgggggc tccgcccgcc ggtggaaaga cctgctcgaa
     4861 gagttcaagg ccgtccggga atcgacggaa tatctttacg acgccctctc cgatgaccaa
     4921 ctgcgcttca ccggcaccgt cagggagctg ccgctgaacg ccttcaccat cggctatatc
     4981 ctccccggtc acgtaaccca tcacctccgc atcctcgaag aacgttatct ctgagcccct
     5041 ccaattctaa ccgacataca tgaacctact tctttctctc ccgccccggc gggcgaaccg
     5101 cattgccgtg agctgtttct tcttcctggc cgggctctgc ttctcctcct gggcttcccg
     5161 tatccccagt atccagcaga aactccatct caataacgtc tccctcggcg gggtactcct
     5221 ggccctcccc tgcggcctga tggcctccct gcccctggca ggctggctcg tggcaaaatt
     5281 cggcagccgc cccatcgcga tcggcgccgg cctcctctac gcctccatcc tccccctgct
     5341 gggctttgcc ggcgccgtct ggcagctggt caccgtcctc ttcttcttcg gcatggccgg
     5401 aaatatgctc aatatcgcca tcaataccca ggcaatcggc acggaatccc tctatggccg
     5461 caccatcatg gcctcctacc atgggctctg gagcctcgcc ggcttctccg gcgcctccat
     5521 cggcggtctc ttcctcggcc tgggctggct cccctggcag cacttcctgg tcatcaccgg
     5581 cgccgcctgg ctccttattt gcttcatggc ccgcaacctg gtcccccggg acctcgccgc
     5641 ggacgagaac cgccccatct tcgcccggcc cgaccgctcc ctgatcaacc tcgggatcat
     5701 cgccttttgc tccatgatct gcgaaggcac catgttcgac tggagcaatg tctatttcca
     5761 aaaagtgatc ttcccaccca aggccctcgc cggcatcggc ctcaccgcct ttatgtccac
     5821 catggccacc ggccgcttcg tcggcgactg ggtcaccacc cggctgggca taaaacgcat
     5881 cctgcaggtg agcgggatcc t
//
LOCUS       DATGFG010000512         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1491464, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000512 DATGFG010000000
VERSION     DATGFG010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491464"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VKQ52_09530"
     CDS             <1..821
                     /locus_tag="VKQ52_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HLZ87470.1"
                     /translation="GTPVTGAIFALEVLALGRIQYDALMPCFMASVLADIVCGAWGIR
                     HTAYHIHFAATGTSFFFHFDLLLLFKVILAGIAFGLAGMAFAETSHAIKKYSSLLIKT
                     KWLVPVAGGLIIIGLTYALGTEDYLGLGVAGKNPDSVTIPSCFHAGGAAGLSWFWKLL
                     FTAITLGTGFKGGEVTPLFFIGAALGNTLALLSGAPVDLLAGLGFIGVFAGATNTPIA
                     CTLMGVELFGADHVLYYAVACFTAYYFSGHTGIYTAQRVGVAKVAARRYGMRKK"
     gene            859..1332
                     /locus_tag="VKQ52_09535"
     CDS             859..1332
                     /locus_tag="VKQ52_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87471.1"
                     /translation="MFRASTSFIKAQIPTASLPLLLDFYKQELEYQRVHSITVSDDAV
                     YFSNRDWSIVGSRFGSQYFCFSTGRILIQDTGSEYHVLLEGDLSRIMWLGGTLAAVCG
                     LATFFASGFNLIAIALGLLIFLLVAVINYVYTLLFFPVYFTSLRNEMEESLQGLP"
     gene            complement(1329..>2459)
                     /locus_tag="VKQ52_09540"
     CDS             complement(1329..>2459)
                     /locus_tag="VKQ52_09540"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLZ87472.1"
                     /translation="TPGNFMCEHIRLSADKKYLLCSANTGTDALDLERRHVLMVSVDK
                     PDMKIMTPGEGLEWGPVLTGDGSALAFISATAQRPPLPAVLRLKDHGGGNIETLCSDR
                     IPADFPQSRLVTPRRVTFTAADGVVVHADLFEPPGGKTRKPAIVYIHGGPPRQMLLGW
                     HYSDYYSNAYASNQYLASLGFVVLAVNYRLGIGYGYEFHKPAKAGAAGASEYRDIKAA
                     GEWLRRQNFVDTGKIGVYGGSYGGFLTAMALAKDSRLFAAGVDFHGVHDWLVQSSIFT
                     ASKDRYEKAPDYEQALRTAWLSSPVSAIDTWRSPVLIIQGDDDRNVRFSESVDLIRRL
                     EKKGVPYESLMIVDDTHHWMNFTNAVTVYAAAADFFVRKFMR"
BASE COUNT          456 a          709 c          713 g          581 t
ORIGIN      
        1 tcgggacgcc ggtgacgggg gccatcttcg cgctggaagt actggcgctt ggcaggatcc
       61 agtacgatgc gctgatgcct tgttttatgg ccagcgtgct ggcggacatc gtttgcggcg
      121 cctggggtat ccggcatacc gcctatcata tccatttcgc ggcaacaggg acgagcttct
      181 ttttccattt cgatctcctt cttcttttca aagtcattct cgcgggtatc gccttcgggc
      241 tggcggggat ggccttcgcg gagacctcgc atgccattaa aaagtacagc agcctgctga
      301 tcaaaaccaa atggctggta cccgtagcag gcgggcttat tattatcggg ttgacatatg
      361 cgcttgggac cgaagactat ctgggtctcg gcgtcgcggg taagaacccc gattccgtga
      421 cgatcccctc ctgctttcat gccggcggcg ccgccgggct cagctggttc tggaaactgc
      481 tctttacagc gatcaccctg ggtaccggtt ttaagggtgg ggaggtcaca ccgctcttct
      541 tcatcggggc agccctcggc aatacccttg cgttattgag cggcgccccc gtggacctgc
      601 tggcgggtct tggctttatc ggggtcttcg ccggcgccac caacaccccc atcgcctgca
      661 ccctgatggg tgtggaacta tttggcgctg accatgttct gtactacgcg gtggcttgtt
      721 tcacggccta ttacttcagt ggtcacaccg gtatctatac ggctcagcgg gtgggcgtcg
      781 ccaaggtggc cgcccgccgg tatgggatgc ggaaaaaata atttgggggg gcgggatagt
      841 tattatcttg ctgtacccat gtttcgagca tccacctctt ttataaaggc tcagatcccg
      901 acagcctctc ttccgctgct gcttgacttc tacaagcaag aactggaata tcagcgcgtt
      961 cattctatta cggtgtccga cgatgccgtc tacttcagca accgggactg gtcgatcgtc
     1021 ggatcgcgtt tcggcagcca gtatttttgc ttctccaccg gcaggatcct gatccaggac
     1081 accggcagcg aataccacgt tcttctcgag ggtgatcttt cacggatcat gtggctgggg
     1141 ggcaccctgg ccgccgtctg tggactggca acgtttttcg cctcgggttt caatttgatc
     1201 gcgatcgccc ttggcctgct tatctttttg ctagtggcgg tgatcaacta tgtctatacc
     1261 cttctcttct tcccggtgta cttcaccagc ctgcgaaatg aaatggaaga gtcactgcag
     1321 ggtctgcctt agcgcataaa tttccgaacg aagaaatccg cggccgcggc gtatacggtg
     1381 acggcattgg tgaaattcat ccagtgatgg gtatcgtcga cgatcatcag ggattcgtag
     1441 ggcacccctt tcttctccag ccggcggatg aggtcgacgc tctcgctgaa ccggacgtta
     1501 cggtcgtcat ctccctggat gatcagcacc ggcgacctcc aggtatcgat ggccgacacc
     1561 ggggaagaaa gccaggccgt ccggagggcc tgttcgtagt cgggggcttt ctcatacctg
     1621 tctttagacg ccgtgaagat cgacgactgg acaagccagt catggacacc gtggaagtcc
     1681 acacccgcgg caaagagccg ggagtctttt gccagcgcca tggcggtgag gaagccgccg
     1741 taagaaccac cgtagacgcc gatcttcccc gtgtctacaa agttttgccg ccgcagccat
     1801 tcaccggccg cctttatatc cctgtactcc gaagcaccgg cggctccggc ctttgcgggc
     1861 ttgtgaaatt catagccata gccgatgcct aaccgatagt tgacggccag gaccacaaaa
     1921 ccaaggctgg ccaaatactg gttggaagcg tacgcgttgg aataataatc cgaataatgc
     1981 cagcccagga gcatctgccg tggcgggcct ccgtggatat agacgatggc cggcttccgg
     2041 gtcttgcccc ccggtggctc gaagaggtcg gcatggacta cgaccccgtc ggccgcggta
     2101 aaggtgaccc ttcgcggcgt caccaatctg ctttgaggaa agtccgccgg tatcctgtca
     2161 ctgcataagg tttcgatatt cccgccgcca tgatccttca ggcgcagaac agcgggtagc
     2221 ggcggccgtt gagccgtcgc gctgataaag gcaagggcgg acccatcacc ggtgaggacg
     2281 ggcccccatt ccaggccttc gcccggcgtc atgatcttca tatccggctt gtcgacgctg
     2341 accatcagga catgccgtct ttcgaggtcg agtgcgtctg tcccggtgtt ggcgctgcag
     2401 agaaggtatt ttttgtcggc gctgaggcgg atgtgttcgc acataaagtt acctggtgt
//
LOCUS       DATGFG010000513         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_233803, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000513 DATGFG010000000
VERSION     DATGFG010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233803"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..492
                     /locus_tag="VKQ52_09545"
     CDS             208..492
                     /locus_tag="VKQ52_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87473.1"
                     /translation="MSVTFDPIINPIVVTLAVLVGVIAGFVIGKGKVAKARSTILRLE
                     NDLVHSNQETLEAQQALVALETHLQEQSSPVIPMKITGSKDNSSKEKASK"
     gene            complement(555..1295)
                     /locus_tag="VKQ52_09550"
     CDS             complement(555..1295)
                     /locus_tag="VKQ52_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLZ87474.1"
                     /translation="MLQPWRTGKVIRIEQEASQTRRFWIQIPEMGSFDFEPGQFVTLD
                     LPIHEKLSKRIRSYSIASWPDGTNVIELVIVLLDGGAGTTYLFNEIRVGSELTLRGPQ
                     GVFTLPDPIEKDLIFICTGTGVAPFRSMSHHILNCTIAHREIYLIFGCRHFGDCLYRQ
                     ELTELAEKIPCFHYIPTFSREKPDPANPLLRTGYVHPIYEEICRVDGGLRPASFFLCG
                     WKDMIDEAKQRIQALGYDRKSIHQELYG"
     gene            complement(1334..>2602)
                     /locus_tag="VKQ52_09555"
     CDS             complement(1334..>2602)
                     /locus_tag="VKQ52_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ87475.1"
                     /translation="KASGAATICGSNATQVVLHAATTGDDVALWYDSQTATVPIAAGN
                     NTSTSEITSNKTYYVALNDLQLKGGAPNKLQFASTSSTLGGAYFRFGGNFVMFTTGVP
                     LTIESAKMYIGHSGKMTFTLATLASLDNSTGGYSYYPLYSTTLDVYATTAHPDTAQQV
                     NVAPGDNTDTGATYLLNIPVPTPGSYIIIIDCANYASAFLNANIKTDPYPLTIPGIFS
                     VTGNDFRDYGKADSVTYSHKFYYPFYNIGIRLPGCPGPRTAVTATTEPSPSISLSGKV
                     LTSTASYGNQWYLGDSALAGHNGVTDTAKFPGLYYTMVTDTVTGCVLTSNKIQFTPTG
                     DANTQIGLTVAPNPSSGTFQLVFYMATADNTSIIIDDMYGQKLYEEDHSGFSGFYNQT
                     INVPNLASGLYILQIIHGGQRYKTTIMIRH"
BASE COUNT          614 a          714 c          671 g          603 t
ORIGIN      
        1 aggtcgcagc tatcgttaac cggcatctgg ggtaaaaaaa taaaaaaata ttgcgggctt
       61 tttgttggaa aagtagaata ttttagtggt atatttgtcc attggtaagg ttctcagaag
      121 cttgccacta tccccagaag agcgtgttgt tattcccttt tgaagtacct gtcttctttg
      181 tcctgaaact taaattttgt aagaacaatg tcagtcactt ttgatccaat catcaaccct
      241 atcgtcgtta cactggcggt tcttgttggt gtgattgcag gttttgtcat tggcaaaggg
      301 aaagtagcaa aagcccgctc caccatcctc cggctggaaa acgatctggt gcacagcaac
      361 caggaaaccc tggaagccca gcaggcgctc gtagcattgg aaacccattt gcaggaacag
      421 tccagccccg ttattccaat gaagattacc ggtagcaagg acaacagctc caaagagaaa
      481 gcctccaagt aaagagaaag cctccaagta gaaagcctcc gggtaaagag cctggccctc
      541 atcaggcaac gccatcaccc atacaactcc tggtgtatcg atttccggtc atagccaagc
      601 gcctgtatcc gctgcttggc ctcatcgatc atatccttcc atccacatag aaaaaagctg
      661 gcgggcctca accctccatc cacccggcag atctcctcgt aaatgggatg cacatacccc
      721 gtccggagaa gcggattagc cggatcgggc ttttcccggg aaaaagtggg gatataatga
      781 aaacagggga tcttctccgc cagctcggtt aattcctgcc ggtagagaca atcgccaaaa
      841 tgccggcagc caaagatcag gtagatctcc cggtgggcga tagtacagtt gaggatatga
      901 tgactcatgc tccggaacgg cgcaaccccc gtcccggtac aaataaagat cagatccttt
      961 tcaatcgggt ccggcaaggt aaacacaccc tgcggccccc ttagcgtcaa ttcactaccc
     1021 acccttattt cattgaacag gtacgtcgtt cccgcccccc cgtccagcaa tacgatgaca
     1081 agctcgatca cgttggtccc gtcgggccag gaagcgatgg aataactgcg gatccgcttg
     1141 ctcagcttct catggatggg aaggtccagc gtcacgaatt gccccggctc gaagtcgaac
     1201 gatcccatct ccggtatctg tatccaaaac ctcctcgtct gggaagcttc ctgctcgatg
     1261 cgtatgacct tgcctgtccg ccagggttgc aacatatcgt taagtttatg gctcccctaa
     1321 tatatagaat tttttagtgt ctgatcatga tcgttgtttt atatcgctga ccaccgtgga
     1381 taatttgcag gatatacaac cccgaagcaa ggttgggcac attgatcgtc tgattataga
     1441 agcccgagaa cccggaatgg tcttcctcgt ataacttctg cccatacata tcgtcgatga
     1501 tgatcgaggt attatccgcg gtagccatat agaacaccag ctggaaagta ccggagctcg
     1561 gattcggcgc aaccgtcagc ccgatctggg tattggcatc acccgttggc gtgaactgga
     1621 tcttgttcga cgtgagtacg caacccgtta ccgtatccgt caccatcgta taatacaatc
     1681 ccgggaattt ggccgtgtcc gttaccccat tgtgcccggc aagcgccgaa tcacccagat
     1741 accactggtt gccataggat gccgtgctcg taaggacctt cccggaaagc gagatgctgg
     1801 gcgaaggctc cgttgtcgcc gtcaccgccg tcctcggtcc cggacaaccc ggtagccgga
     1861 tgccgatatt atagaacgga taatagaatt tatgcgaata cgtgacgctg tcggccttgc
     1921 catagtcacg gaaatcatta cccgtcaccg agaagatccc cggtatcgtc aacgggtagg
     1981 ggtccgtctt gatattggca ttgaggaatg ccgatgcata attggcgcag tcgatgataa
     2041 tgatatagct gcccggcgtc ggcacgggaa tgttcaacag gtaggtcgcc cccgtgtcgg
     2101 tattgtcccc cggcgcgaca ttcacctgct gggccgtgtc gggatgcgcc gtggtggcat
     2161 agacatcgag cgttgtggaa tacaggggat agtaggaata cccgcccgtg ctgttgtcga
     2221 gactggccag ggtcgccagc gtaaaggtca tcttgcccga atgcccgata tacatcttcg
     2281 cactctcgat ggtcagcggc accccggtcg tgaacatcac aaaattaccg ccaaaccgga
     2341 aataagcgcc gccaagcgtg ctgctcgtgc tggcgaactg cagcttgttc ggcgcgccgc
     2401 ccttcagctg cagatcgttc agcgctacat aataggtctt gttgctcgtg atctcggagg
     2461 tggacgtgtt attgcccgcg gcaatcggta cggtagcggt ctggctgtcg taccagaggg
     2521 cgacatcatc acccgtggtc gcggcatgca gcaccacctg ggtggcgttg ctgccgcaga
     2581 tggtggccgc accactggcc tt
//
LOCUS       DATGFG010000514         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_919944, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000514 DATGFG010000000
VERSION     DATGFG010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919944"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3017)
                     /locus_tag="VKQ52_09560"
     CDS             complement(<1..>3017)
                     /locus_tag="VKQ52_09560"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HLZ87476.1"
                     /translation="SPWQRRMADEPASPGFDDHAWLASEWTLQMGADGDGGADAWYRT
                     AVRIDSGGEYTLQAVGGDRATVFVDGVVAASGNLHEGSFRLALTKGSHTIAVFTAHDG
                     RDKLAGFLGSMMDVDPKGLAGEMRLEKGAPTLQRLAGWRFLPAAAPLPDGAPGGVGVS
                     GAAGAAALILPTGKETGWRDYTIGDDAFGKREGFGWFRVVLPEPPAGAGSGVLDFRST
                     DENATVFLNGRRLLRHEGWNLPFRVNLDGLDTMRRPLVLTVFIENYSNEGGIDRPVQV
                     HYFSESREIKGWRMHGGIGEPGMIANWSAMEGRPVMEDRAAMEDRSAMETGRPGAPCY
                     YRTEFRVPPYEARGEHPIWRVHIDGLGHGSVWVNGHNLGRYPEKTPAPGEYIPECWLK
                     DGVNELVIYDEDGRRPDSVTVRRERAAGRRLTWYSAGKEVADYVDPMIGTAKSAVPTK
                     WGNEGGTYPGAVAPWGFVQMTPETRVDGGYDHADAAIGWFSCIGHMSGYPGGSAGRGK
                     IMPVVEAAGSMPENRQGPAPRAFLHTDEVASPGYYRVLFRDNNTLVETTASERVGWMR
                     CSFPRGVLPRVYIAGLDGIAALHFDRPVVREEKTGGGRVLTFVAARDSATVVEIAVAV
                     SMVSIANAEGNWRAAGEKHRFDEVARQTREKWRKVLSVVDVEDGKEEDKIIFYTALYH
                     ALLFPWIISDADGQYRGADRQIHRVSGREAYGGFSPWDSFRSQQPLLTLLFPGRERDI
                     ILSMLDVYRQTGYLPSEPMTGNHGVPIIVDAYRKGIRGFDPMEAYAAMRKGIVDKPYR
                     PADREAYRRLGYLPLSYPESVTRTVEYAYDDWVLSQFAKEVLHRDGDADDLIKGSYGY
                     RNVFDVDSLFFLPREGGRFLRRPGTSGYKEGDQWAYTYFDLQYPVDLVNLLGGDREFV
                     RRLDGALANGDIVFDNETVFHVPYLFQYAGASGKTREWVRAFRDGRFSATPGGLPGND
                     DLGAFSSWYVFSAMGFYPLCPGRPEYVTGTPLF"
BASE COUNT          677 a         1176 c          617 g          547 t
ORIGIN      
        1 ggaataacgg cgtcccggtc acatattccg gacggccggg acacaaagga taaaagccca
       61 tcgccgaaaa aacataccag ctcgaaaaag cacccaggtc atcatttccc ggtaacccac
      121 ccggtgtagc gctaaaccgg ccatccctga acgcccggac ccactcccgc gttttgccgg
      181 acgccccggc atactgaaaa agatacggca catgaaaaac cgtctcattg tcaaaaacaa
      241 tgtccccatt cgccagcgcc ccatcgagcc gccggacgaa ttcccggtcc ccgcccaaca
      301 ggttgacgag gtccacagga tattgcagat cgaaatacgt atacgcccac tgatcccctt
      361 ccttataccc gctcgtcccc ggccgccgca ggaaccgtcc gccctcccgc ggcaaaaaga
      421 aaagcgaatc cacatcgaac acattccggt aaccataact cccctttatc aaatcatcag
      481 catccccatc ccggtgcaac acctccttcg cgaattggga caacacccag tcatcatacg
      541 catactccac cgtgcgcgtc accgactcgg ggtacgaaag cggtaaataa cccaatcgcc
      601 ggtaagcctc gcggtccgcc ggccggtagg gcttgtccac gatccctttc cgcatggccg
      661 cataagcctc catcggatca aatcccctta tacccttgcg gtaagcatcc acgatgatcg
      721 ggacaccgtg atttcccgtc atcggctccg agggaagata acccgtttga cggtacacat
      781 ccagcatcga caggatgata tcccgctccc gcccggggaa caacagcgtc aataacggct
      841 gctgactgcg gaaactgtcc cagggcgaaa acccgccata cgcttccctc cccgataccc
      901 ggtggatctg ccgatccgct cccctgtatt gcccatccgc atcggaaatg atccagggga
      961 acaacaaagc atgatacaac gcggtataga aaataatttt atcttcttcc tttccatcct
     1021 ccacgtccac caccgacaac acctttcgcc atttctccct cgtctgccgc gctacctcgt
     1081 caaaacgatg cttctccccc gcggccctcc agttcccttc tgcgttggcg atactcacca
     1141 tactaacggc aaccgctatc tctacaacgg tagcgctatc cctcgcggca acaaacgtca
     1201 acacccggcc tccacccgtt ttttcttccc tcacgaccgg ccggtcgaaa tgcaaggcgg
     1261 ctatcccatc caatccggcg atatataccc ggggcagaac cccacgtggg aagctgcacc
     1321 gcatccagcc cacccgttcc gacgccgtcg tctccaccag tgtattatta tccctgaaca
     1381 acacccggta atagcccggc gacgccactt catccgtatg aagaaaagcc ctcggcgccg
     1441 gaccctgcct attctctggc atcgaccccg cagcctccac gactggcatg atcttccccc
     1501 tccccgccga accacccgga tacccactca tatgcccgat acaactaaac cacccgatcg
     1561 ccgcatcggc atgatcatac ccgccatcca cccgggtctc gggtgtcatc tgcacaaatc
     1621 cccagggcgc cacggccccg ggataggttc ccccttcgtt tccccatttg gtgggaacag
     1681 cactcttcgc cgtcccgatc atcgggtcca catagtcggc cacttcctta ccggccgaat
     1741 accaggtcaa cctccggccc gcggcccgct cccggcggac agtgacacta tcgggccggc
     1801 gaccgtcctc atcgtaaata accagttcat tcaccccatc cttcaaccag cattcaggaa
     1861 tatactctcc gggtgcaggc gtcttctccg ggtaacggcc aaggttatgt ccattcaccc
     1921 acaccgaccc atgccccaac ccatcgatat gcacccgcca gatcggatgt tcaccccgcg
     1981 cttcatacgg aggcacccga aattccgtcc ggtaataaca aggcgctccc ggcctgcccg
     2041 tctccatcgc cgacctgtcc tccatcgccg cccgatcctc cataactggc cggccttcca
     2101 tcgccgacca attcgcgatc atccccggtt cgccgatccc cccatgcatc ctccatccct
     2161 ttatctcccg cgactcactg aaataatgca cctgcaccgg ccggtcgatc ccgccctcat
     2221 tcgaataatt ctcgataaac accgtcaata ccagcggcct ccgcatcgta tccaatccat
     2281 ccaggttcac cctgaacggc aaattccaac cctcatgccg caaaagccgc cggccattga
     2341 gaaacaccgt ggcattctca tccgtgctcc tgaaatccag caccccgctt cccgcgccag
     2401 ccggcggttc cggcaacacc acccggaacc acccaaatcc ttccctcttc ccaaacgcat
     2461 catccccgat ggtataatcc cgccaacccg tttcctttcc cgtcggtaag atcaacgccg
     2521 ccgcccctgc cgcccccgaa acacccacac ctccgggcgc cccatccggc aacggcgccg
     2581 ccgccggcaa aaacctccac cccgccagtc gctgcagcgt cggcgccccc ttctccaacc
     2641 gcatctcgcc cgcaagccct ttgggatcga catccatcat cgatcccaaa aaaccggcaa
     2701 gcttatcacg cccatcgtgc gccgtaaaca cagcgatagt atgactcccc tttgtcaggg
     2761 ccaggcgaaa acttccctcg tgcaaattgc ccgacgccgc aaccactcca tccacaaaca
     2821 ccgtcgcccg atcgccgcca actgcctgta acgtatattc gccaccggaa tcgatccgca
     2881 ccgccgtacg ataccaggca tccgcgcccc cgtccccatc agcgcccatc tgcaaagtcc
     2941 actcactcgc caaccaggca tgatcatcaa aacccggcga cgccggctca tccgccatcc
     3001 gccgttgcca gggcgac
//
LOCUS       DATGFG010000515         8847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_30991, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000515 DATGFG010000000
VERSION     DATGFG010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30991"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VKQ52_09565"
     CDS             <1..477
                     /locus_tag="VKQ52_09565"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ87477.1"
                     /translation="KQYALVRTSRNILFDFVRNEVGEEELNRRFPAYADKSISDLLVH
                     SASCYFHWLAYLALQQPRGSLYEGGTTMEELHRLYEQVDTTMALFLEHFAASMDESFD
                     GVHDDGWRVRVTPLELFTHVTTHEYHHKGQIVLICRMLGHVPVDTDASNAFEEGKK"
     gene            600..1103
                     /locus_tag="VKQ52_09570"
     CDS             600..1103
                     /locus_tag="VKQ52_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLZ87478.1"
                     /translation="MMKREAVFTKDFQSKKITVVREFDAPLDLVWKAWTDSAILDQWW
                     APRPWRAETKKMDFRAGGKWLYNMIGPDGNGAWCVVAFHKVDANRGFSSSAAFCDEAG
                     NTNSDFPVMEWQSEFSQTGGVTTVRVEISFAKEADMQTIIAMRFLEGFAMGLGNLEEY
                     LQAHVPA"
     gene            complement(1234..3633)
                     /locus_tag="VKQ52_09575"
     CDS             complement(1234..3633)
                     /locus_tag="VKQ52_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87479.1"
                     /translation="MIKNFFTTAWRNLLKNKAFSLINISGLAIGMAIALLIGLWVLDE
                     LSFDSYHRNHDRIAQVMQHQTLNGDISTFGTMSYPVAGELRRSYGSDFKYVVMTSVPG
                     SNFLTYGEKKIPVSGLFMEPAGPKMLTLNMLEGSLDGLDDPHSILLPASMAKTLFGDK
                     PALNNSLTIDNKQQVRVTGVYEDLPDNSSFKSIGFFAPWELYRTSAQWIINSANIWDN
                     NSFQVFVQIADNTGFEAVTKKIADSKQAHVDPEDKKFRTKLFLHPMNDWHLRSHWDSN
                     GVKAGGLITYVWLFAIIGVFVLILACINFMNLSTARSEKRAKEVGIRKTIGSLRWQII
                     GQFYSESLLVVAFAYFLSILLVRLALPFFNGIAGKRMTIPYGHPLFWLLGIAFTIVTG
                     FIAGSYPALYLSSFRPIKVLKGTFKAGRIAALPRKVLVVLQFTISIALAIGTIVVYEQ
                     VQYTKNRPIGYDRNGIMMLKMKSADFYGKYGLLSAELKSSGAVRDFAESSSPVTEVWA
                     TGDIFSWPGKDPNQNVDFANIWVTPEYGRTVGWQISRGRDFSEALRTDSSSVVINEAA
                     VRSMGLKDPIGASITSGSGAHTQTFRVIGVIRDILMESPFQPVKQTIYFLDYDNVNWI
                     ILRLNPQQSASASVAKIAAVFKKLIPAAPFDYKFADTEFAAKFGAEERIGRLSTIFAS
                     LAIFISCLGLFGLASFVAEQRTREIGVRKVLGASLFSVWKLLSRDFVLLVGISVLIAT
                     PLAWLFMNNWLREYPYHMDLSWWVFTAVAAGALVITILTVSFQAIRTALANPVNSLRS
                     E"
     gene            complement(3746..4963)
                     /locus_tag="VKQ52_09580"
     CDS             complement(3746..4963)
                     /locus_tag="VKQ52_09580"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HLZ87480.1"
                     /translation="MSTHRSFPAVPTAILTAIFILSLTFSIHAQSAHVNPWAPTDHGP
                     LPPISSLTNGVWLKGELHVHSRHSKESSNNPIAKIIAFSRSVGMDYVCITDHDNHVEG
                     DVEHNTWADPEFHSDSVLLLYGAEWTTTRGHGNVFSARPYDHQRLYDVRDQRDIVVGA
                     IVKELGIHLSANHPSGKDHFGYSYDMVKSIEVWNSAIWSKNANAIMIWDDMLSSGRKL
                     TGRGGSDAHHGVPETPDKATKNSYQAKANYVGTPTTWVFATERTAQAVVDALTNGRVC
                     VTANPYAPRVEFYADLDQDGKMDMMMGDNAKATGRPVRFHIQLTGNTASDSAYTVNVI
                     KNGDPFNTYKTSGKIPTVEFTDTPASIGRTYYRVEVQGPPTPYPQVPGSMALSGNMVG
                     LSNPLYFNFDPNF"
     gene            complement(5069..5776)
                     /locus_tag="VKQ52_09585"
     CDS             complement(5069..5776)
                     /locus_tag="VKQ52_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ87481.1"
                     /translation="MSIKGLFPIEKWDFGSESILADLPERDKELLMARKLEQVYKNGE
                     IIFREETYPTGIFFLVNGKAKKYKVDKDGREQIIYVAHSGELLGYHAILSGDNYPDSA
                     AVMKDSLIAFIPREDFIEAIRQSDILNRRLLKTLSHEFVVLTNSLALFAQRSVRERLA
                     LQLIVVREKYKEDFEPGMPVEINMSRDDLASLVGTARENVVRMLSEFKEDGIVETKGR
                     KIIVRDVKRLIKVANLS"
     gene            5895..6191
                     /locus_tag="VKQ52_09590"
     CDS             5895..6191
                     /locus_tag="VKQ52_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87482.1"
                     /translation="MKNPRAPRLTARRLQELKDLGFRFVLIKGYTIDRRSDYIELNHF
                     TLVPVKELPEDPGQKEIYEPIESEILLEWASSPDNGVMAFIESDQMKLPNGDYS"
     gene            complement(6168..6956)
                     /locus_tag="VKQ52_09595"
     CDS             complement(6168..6956)
                     /locus_tag="VKQ52_09595"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLZ87483.1"
                     /translation="MKGAQFLFFPLAWITFADCNNPTSKKEDRVIHINHIVDLTHTLT
                     AEFPYIPVKNLTYPFKLIPMATLKDNGVAANSWHIHEHLGTHIDAPNHFINDQKSVDQ
                     IALQDLIVPVEVIDIEEKASKNRDAELTVGDLEQFESKNGRIPEHSCVIMYSGWEKHL
                     KDSLFVGLDDRQVKHYPGFSNAAIQFLIKERNIAGIGVDVLSFDPGIDENYTGHKTLF
                     AAGKWGVECVANLSKIPPTGATIIVGAPKVGQATGGFSRIIAVW"
     gene            complement(7013..7567)
                     /locus_tag="VKQ52_09600"
     CDS             complement(7013..7567)
                     /locus_tag="VKQ52_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLZ87484.1"
                     /translation="MNRRLFLQLPLAASALVAEAQDPVKTRSKQGFKVEKGKDHFQEE
                     LLIMGGQFDCKVSAKDTNGDLCIYDTTRQEKGGPVLHVHFNQDEWFYIIKGEFIARVG
                     EDTLSLKPGDSAFAPRKIPHAFAKISEGEGQMLVLFQPAGSMEDFFKQMSKLGKNIPK
                     DQERALKNLWEAHGMAMVGPPLKF"
     gene            7758..>8847
                     /locus_tag="VKQ52_09605"
     CDS             7758..>8847
                     /locus_tag="VKQ52_09605"
                     /inference="COORDINATES: protein motif:HMM:NF020768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HLZ87485.1"
                     /translation="MKAALPLFFCFFLMLPATLWAGNSLDKVGNPTATPLVAFSLRQL
                     SSAYTGKAIQVRRSSDNASQDIGFTATGNLDTAALKTFVGSGNGYVSIWYDQSGNGYN
                     ATQTTVANQPVIMSGGVINRDNGQPSVYTSATGFLTYGPVSQLSGTTQVTRMEVCRSR
                     DANNLAITEGLGTYQLDLQFFPTKIWVQFETGNIVANAAVSNPTTLMSINSVRNSGAS
                     QLYVNTALLGSATATIMTFSAPVTGYIGVRFDYLSSNTGIPGAFSETILFNSVLSDAD
                     RQAMNYNENWYYSLGFDPCSSTAASLSANGTTSRALYACTQDGPYAYYYDPAHPLKLL
                     FGIAKDPGSSGANPSFVVDSINLTTTSDP"
BASE COUNT         1948 a         2185 c         2516 g         2198 t
ORIGIN      
        1 aaacaatatg cactggtgcg gacctcgaga aatattcttt tcgactttgt ccggaacgag
       61 gtgggggagg aggaactgaa ccggcgtttt cctgcttatg ccgacaagag catcagcgat
      121 ctgctggtac atagcgcgtc ctgttatttt cactggctgg cctacctggc gctgcaacag
      181 ccgcggggat ccctgtatga gggtggcacg acgatggagg aattgcaccg cttgtatgaa
      241 caggtggata cgacgatggc gcttttcctg gaacattttg ccgcgagcat ggatgaatct
      301 tttgatgggg ttcatgatga tggctggcgg gtgagggtaa ccccgctgga gctttttacg
      361 catgttacga cccacgaata tcatcacaag gggcagatcg tgctcatctg ccggatgtta
      421 gggcatgttc cggtggatac ggatgcgagt aatgcatttg aggaggggaa aaaataatgg
      481 cggaaaaatt ttcgcaaccg aatgtttgcg catatatttg caactgaacg gttgctaata
      541 gagcccccgt tgcccggcat tttggataac ctattggaaa atttaaaaaa taaaaagcca
      601 tgatgaagag agaagcggtc tttaccaaag actttcaaag caagaaaatc accgttgtca
      661 gggaatttga tgccccattg gacctggttt ggaaagcctg gaccgacagc gcgatcctcg
      721 accagtggtg ggcgcccaga ccctggcggg cggagacgaa gaaaatggat ttcagggccg
      781 ggggtaaatg gctatacaac atgatcgggc ccgatggtaa tggcgcgtgg tgtgtcgtcg
      841 cgtttcacaa ggtggatgcg aacaggggat ttagcagttc ggctgctttt tgtgatgaag
      901 cggggaatac caattcggat tttcccgtca tggaatggca gagcgagttt agccagaccg
      961 ggggcgtgac gacggtgcgg gtggagatca gcttcgcaaa ggaagcggat atgcagacga
     1021 tcatcgcgat gcgcttcctg gaaggtttcg ccatgggtct tggcaacctc gaagaatacc
     1081 tgcaggcgca tgtaccggcg taaggtttat ggggctcctg ttccgggcgg agggtggtga
     1141 atcatcccgc gcggaagccg atggtcattc cgatcggagg gtggtgatta tgccgcgcgg
     1201 aagccgatga ttgtttcgat cggagggtgg tgattattcc gatcggaggc tgttgaccgg
     1261 gttggccagt gctgtccgga tggcctggaa gcttaccgta aggatggtaa tgaccagggc
     1321 gccggcggcc acagccgtga agacccacca ggacaggtcc atatgatagg gatattcccg
     1381 cagccagttg ttcataaaaa gccaggcgag cggggtggcg atcagcaccg aaatgcccac
     1441 gagcaggaca aaatccctgc tcagcaattt ccagacgctg aataaggagg cacccaacac
     1501 ctttcgcacc ccgatctccc tggtgcgctg ttcggcgacg aaggaggcca gcccgaacag
     1561 gccgagacag gagataaaga tggcaagcga tgcaaaaatc gtagagagcc ggccgatgcg
     1621 ttcttcggcg ccgaattttg cggcgaactc ggtatccgcg aatttataat cgaagggcgc
     1681 ggcggggatg agctttttga agaccgccgc aatttttgcg acggaggcgc ttgcgctttg
     1741 ctgtgggttc aggcgcagga tgatccagtt gacgttgtca tagtcgagga aatagatcgt
     1801 ctgtttcacc ggctggaagg gggattccat cagtatatcc ctgatcacgc caatgaccct
     1861 aaaggtttgg gtgtgcgctc cggaacccga tgttatgctg gcgccgatag ggtctttcag
     1921 ccccatggat cttaccgcgg cttcgttgat gacgaccgaa gaggagtctg tccggagggc
     1981 ttcagagaaa tcgcgacccc tactgatctg ccagccgacc gttcgtccgt actccggggt
     2041 gacccatatg ttggcgaagt caacgttctg gttggggtct ttcccgggcc aggagaatat
     2101 gtcgccggtg gcccagactt ccgtaaccgg actcgatgac tcggcgaaat cccggacggc
     2161 ccccgaagat ttcagctcgg cgctcaacaa accatatttc ccgtagaagt cggcggattt
     2221 cattttcagc atcatgatgc cgttgcggtc gtagccgatg ggccggttct tggtatactg
     2281 cacctgctca tagacgacga tcgtcccgat ggccagcgcg atggagatcg tgaactgcag
     2341 gacgaccaat actttccggg gcagggcggc gatcctgcct gccttgaaag ttcctttcag
     2401 cacctttatc ggcctgaagg aagagaggta taatgccggg tagctgccgg cgatgaaccc
     2461 ggtgacgatc gtaaaggcga tgcccagcaa ccagaacagg ggatgaccat agggaatggt
     2521 catccgcttc ccggcgatgc cgttgaagaa gggcagggcc agccggacca gcaggatgga
     2581 gagaaagtag gcgaaggcta ccacgagcag cgattcacta tagaactggc cgatgatctg
     2641 ccagcgcagg gagccgatcg ttttccggat gcctacctct tttgcccgtt tttcgctgcg
     2701 ggccgtgctg aggttcataa aattgataca ggcgaggatc agtacgaaga cgccgatgat
     2761 cgcaaacagc catacatagg tgatcagccc gcctgccttt acaccgttgc tatcccaatg
     2821 cgaacggaga tgccaatcat tcatgggatg caggaagagc tttgtccgga atttcttatc
     2881 ctctgggtcc acatgggcct gtttactatc cgcgatcttt ttcgtcaccg cctcgaagcc
     2941 ggtgttgtcc gctatctgta cgaacacctg aaaggagttg ttgtcccata tgttcgctga
     3001 gttgatgatc cattgcgcag aggtgcgata gagttcccag ggggcaaaaa agccgatact
     3061 cttaaagctg ctgttatcgg ggagatcctc gtaaaccccg gtaaccctta cctgttgttt
     3121 attatcgatg gtcaggctat tgttgagggc cggtttgtcc ccgaaaaggg tctttgccat
     3181 ggaagcgggc agcaggatcg aatgtgggtc gtccagcccg tccagacttc cttcgagcat
     3241 gttcagggtg agcatcttcg gaccagccgg ttccatgaac aggccgctga cggggatctt
     3301 tttctcgcca taggtgagga aattcgagcc gggcacggag gtcatcacca catatttaaa
     3361 atcgctccca tagctgcgcc tgagctcacc ggcaaccgga taggacatcg tgccaaaggt
     3421 ggagatatcc ccgttcaggg tttggtgctg catcacctgg gcgatgcgat cgtggttccg
     3481 atggtaactg tcgaaggaaa gctcgtcgag cacccataga ccgatcagca gggcgatggc
     3541 cattcctatg gcgaggccgc tgatattgat cagggaaaat gctttgttct tcagcagatt
     3601 gcgccaggcg gtggtaaaga agtttttgat catatccttc cgtggcgcca ataaaatgcc
     3661 aaatggccaa tcggctgcaa atcaaccgcc tatttcgctt tccgccgggt tgactgtccg
     3721 gttttgatac tccgggtgtc cgcgatcaga agttggggtc gaagttgaaa taaagagggt
     3781 tcgacaaccc gaccatatta ccgctgagcg ccatcgatcc gggcacctgt ggataggggg
     3841 ttgggggtcc ctgtacttcg acccggtaat aggtgcggcc gatggatgcc ggcgtatcgg
     3901 tgaattcgac ggtggggatc ttgccgctcg tcttgtaggt gttgaagggg tcgccgttct
     3961 ttatgacatt gacggtgtag gcagaatcgc tggccgtatt cccggtgagc tggatatgga
     4021 atctgacggg cctgcccgta gcctttgcat tgtcgcccat catcatgtcc attttgccgt
     4081 cctggtccag atcggcgtag aactcgacgc ggggtgcgta gggattggcg gtgacacaga
     4141 cccggccgtt tgtcagggcg tcgactactg cctgtgccgt ccgttcggtg gcgaataccc
     4201 aggtggtggg tgttccgaca tagttggctt ttgcctggta gctgttcttt gttgccttat
     4261 cgggtgtttc cgggacgcca tggtgtgcgt cgcttccacc cctgccggtg agtttgcggc
     4321 cggaggacag catgtcatcc cagatcatga tcgcgttggc gttttttgac cagatggcgg
     4381 aattccagac ctcgatggat ttgaccatat cataggaata gccgaaatgg tcttttccgc
     4441 tggggtggtt ggccgacaga tggataccca attcttttac gatggcgccg accacgatgt
     4501 cccgctggtc gcggacgtca tagagccgct ggtgatcata gggtctggcc gaaaatacat
     4561 ttccatgacc gcgtgtcgtc gtccactcgg cgccatacaa tagcaggacg gagtcagaat
     4621 ggaactcggg gtcggcccag gtgttgtgtt cgacatcgcc ttccacatgg ttgtcatggt
     4681 cggtgataca gacataatcc ataccgacgg atcgggagaa ggcgatgatc tttgcgatgg
     4741 ggttgttcga ggactccttg ctatgccggg agtggacgtg gagttctcct ttgagccata
     4801 cgccgttggt aaggctggag atggggggca gcggaccgtg gtctgtaggc gcccaggggt
     4861 tgacatgggc ggattgtgcg tggatggaga aggtaaggct gaggatgaag atggcggtga
     4921 gaatagccgt ggggacggcc gggaaagatc tatgtgtgct catttgttct gctttccccg
     4981 atagaacggt tggcggggca atggatgact atgcgggcat gttagcaaat tgttatctgt
     5041 ccgatataac gtcgaacgcg gtttgccgct agctgaggtt ggcgaccttg atcaggcgct
     5101 ttacgtcacg cacgatgatc tttcttcctt ttgtctcgac gatcccatct tctttgaact
     5161 ctgaaagcat ccgcacgaca ttttctcttg cggtgcccac gaggctggcc aggtcgtcac
     5221 ggctcatgtt tatttcaacg ggcatgccgg gctcgaaatc ctctttatat ttttcccgga
     5281 cgacgatcaa ttgcagggcg agcctttccc tgaccgatcg ttgggcgaac agcgccaggc
     5341 tgtttgtcag gacgacgaac tcgtggctta gtgtcttgag cagtcttctg ttcaggatgt
     5401 ccgactggcg gatggcctcg ataaaatctt ccctcgggat gaatgcgatc aggctatcct
     5461 tcatcacggc ggccgagtcg gggtagttat ccccggagag gatcgcatga taacccaaca
     5521 attcacccga atgggctaca tagatgatct gttcgcggcc atccttatcc actttatact
     5581 tctttgcctt gccgttcacg agaaagaaaa tacctgtggg ataggtttct tccctgaaga
     5641 tgatctctcc gttcttatac acctgctcca gtttcctggc catcaacagc tccttgtccc
     5701 gctcggggag atcggcgagg atcgattcac tcccgaaatc ccatttttca atcggaaaga
     5761 gacccttgat gctcataggc acaaatctag ccttttaaaa attgatttta ctcacttttt
     5821 tcagtgacaa ttctcaccgc gggttctggg acattcgact agttttgatc atcccaaaac
     5881 caaaatgaag tggtatgaaa aacccaaggg cgccaaggtt gacggcacgc aggcttcagg
     5941 aattgaaaga cctcgggttc aggtttgttc ttataaaggg ctacacgatc gacaggcgtt
     6001 ccgactacat cgagctgaat cattttaccc tggttccggt gaaagagttg cccgaagatc
     6061 ccgggcagaa ggagatttat gagcctatcg aaagcgaaat tttgctggag tgggccagct
     6121 ccccggacaa tggcgtcatg gcatttattg aatcggatca aatgaagcta ccaaacggcg
     6181 attattcttg aaaaacctcc tgttgcctgg cctacttttg gtgcgccgac aatgatcgtg
     6241 gcgcctgttg gcggaatttt ggagagattt gccacgcatt ccactcccca tttgccggcg
     6301 gcaaataatg ttttgtggcc ggtgtaattt tcgtcgatgc cggggtcgaa ggagagtaca
     6361 tccacgccga taccggcgat atttctttct tttatcagaa attgaatcgc ggcgttggag
     6421 aaaccgggat aatgtttgac ctgccggtca tccaatccca cgaacaggga atctttcagg
     6481 tgtttttccc agccggaata cattattacg caggaatgtt cggggatccg tccatttttg
     6541 gattcgaatt gttcgagatc cccgaccgtt aattcggcat cccggttttt cgatgctttt
     6601 tcttcgatat cgataacctc taccgggacg atcagatcct gtaaggctat ctggtcaacg
     6661 gatttctgat cgttgatgaa atggttgggt gcatcgatat gggttcccag atgttcgtga
     6721 atgtgccagg agtttgccgc gacgccgtta tcctttagcg tagccatggg aataagctta
     6781 aaaggatagg tcaggttctt taccggtata taggggaatt cggcagtaag ggtatgcgtc
     6841 aggtctacga tgtggttaat atgaataacg cggtcttctt ttttcgaagt cggattattg
     6901 cagtcggcaa aggtgatcca tgcaagtgga aaaaataaaa attgcgctcc cttcatatcc
     6961 aaatctacaa ttattcccct ggatggggaa cggagagcgt tggcctttcg cgctaaaatt
     7021 tcagcggagg acccaccatt gccattccat gggcttccca caggtttttc agcgcgcgtt
     7081 cctgatcttt cggaatattt ttacccagct tgctcatttg tttaaagaag tcttccatgg
     7141 accccgcggg ttgaaagaga acgagcattt gcccttctcc ttcgctgatc tttgcaaagg
     7201 catggggaat ctttcttggc gcgaaggcgg agtctcccgg cttcagcgat agggtatcct
     7261 ccccgaccct ggcaataaac tcccctttga ttatatagaa ccattcatcc tggttaaaat
     7321 ggacatggag taccgggcct cccttttcct gccgggtggt atcatagata cataaatccc
     7381 cgttggtatc ctttgcggaa accttgcagt cgaattggcc gcccatgatc aggagttctt
     7441 cctggaaatg gtcctttcct ttttctactt taaatccttg cttcgatctg gtcttgactg
     7501 gatcctgggc ttctgccacc agggcagatg ccgccagcgg gagctggagg aataaacgtc
     7561 ggttcatatt ggttgggttt tgggtggaga tgggttgcca gggtataagt tatgcatttt
     7621 tttaaactta atatcaaata ggtaggtgtt ctttttatac caaaccaaac tattacgatt
     7681 gtccatgccc ccctgccggc ctagcttcgc atttgaaatt ccggccccta taaaagcaga
     7741 tgttttaatc caattccatg aaagcagcct tacccctgtt cttttgcttt ttcctgatgc
     7801 tgccagccac cctgtgggca gggaattccc tcgataaggt tggtaatcca acggcgaccc
     7861 cacttgtcgc attcagcctc cggcaactca gctcggcata tacaggcaag gccatccagg
     7921 tgcggcgcag cagtgacaat gcctcacagg atatcggctt tacggctact ggaaacctgg
     7981 atacggctgc gctgaagacc tttgtgggga gcggcaatgg ttatgtgagc atttggtatg
     8041 accaaagcgg caatggttat aatgcgaccc agaccacggt cgccaatcaa ccggtgatca
     8101 tgagcggcgg cgtgatcaac agggataatg gccagccttc cgtgtacacc tctgctaccg
     8161 gcttcctgac atacgggcca gtaagccagt taagcgggac cacccaggtc acgcgtatgg
     8221 aggtttgccg gtcccgcgac gccaacaacc ttgccattac cgaaggattg ggtacctacc
     8281 agctggatct gcaattcttc cccactaaga tctgggtgca gtttgaaacc ggtaatattg
     8341 ttgccaacgc ggccgtgagc aatccgacca cgctgatgtc gatcaattcc gtgaggaaca
     8401 gcggggcctc gcagctatat gtcaacacgg ctttactcgg ttccgccact gcgaccatta
     8461 tgacattcag cgcacccgtg acaggctata tcggggttcg gtttgattac ctttcaagta
     8521 acaccggcat acctggtgct ttttctgaaa ctattttatt caattcggtc ttatcggatg
     8581 cggaccggca ggcgatgaat tataacgaga attggtacta ttcgctgggg ttcgacccat
     8641 gcagcagtac agcggccagt ctttctgcga acggtacgac ttccagggca ctgtatgcct
     8701 gtacgcagga cggtccttat gcatactatt atgatccggc ccatccgttg aagctgttgt
     8761 tcgggattgc gaaagaccct ggcagcagcg gggcaaaccc ctcgtttgtg gtagactcga
     8821 tcaatctgac gacgacctcc gatccaa
//
LOCUS       DATGFG010000516         6048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_440979, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000516 DATGFG010000000
VERSION     DATGFG010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440979"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /gene="rnr"
                     /locus_tag="VKQ52_09610"
     CDS             complement(<1..2019)
                     /gene="rnr"
                     /locus_tag="VKQ52_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HLZ87486.1"
                     /translation="MKNKKKDKNRRKAPADSANLLQGRLDVTRSGMGFVIVEGRDQDI
                     LVRPADFNTALHGDTVRVRVVSAGGRSGRAQGEVVDVVDRKQLEFLGRIEISQSFAFF
                     IAESDKPMPDIYVPLSRLNGAVNQDKVIVRITEWEKNKKPQGEVVQVMDKSDQNDLAM
                     KDILLENGFPIFFPENVLEESGRLPDLIPGAEIANRRDMREVLTFTIDPVDAKDFDDA
                     ISLRRVDKGEWEIGIHIADVSYYVEPETALDKEAFTRATSVYLPDRVNPMLPERISNE
                     LCSLRPHEDKLTFSAVFHMTADAKVKSSWLGRTVIHSDHRFTYEEVQEIIEQKTGLYQ
                     PEIELLNDIAQRLRKHRFRKGAINFSSTEVRFKLDPSGKPVGIMVKESKESHQLVEEF
                     MLLANRHVAEAVAKIKVNKKPIPFPYRVHDTPDKEKLLPFVEFAKKYGHVFDMQTPQA
                     IAKSFNQMLQDVKGRPEQHVLEQLGIRTMAKAVYTTENIGHYGLGFEHYCHFTSPIRR
                     YPDVMVHRVLQEVLEGKTEVDKKMESKCKQSSERERAAMESERAANKYKQVEYMQSFL
                     GDEFEGVISGVASFGFWVETVEHKCEGLVSLNSLLEYDDFRHVETDYALVGRRSGRQF
                     RMGDKVTIKVVAANLTKRQLDYEWVITAAEKEAVRMEPRAASGPAQAQE"
     gene            complement(2016..3083)
                     /gene="lpxK"
                     /locus_tag="VKQ52_09615"
     CDS             complement(2016..3083)
                     /gene="lpxK"
                     /locus_tag="VKQ52_09615"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HLZ87487.1"
                     /translation="MNFNFPLLKPIRILLFPFSLAYALVVTIRNWCFDKKIFPSTSFN
                     LPIICVGNLAAGGTGKSPMVELLIRLLKDRYAIAVLSRGYKRKTRGYALANASTTALD
                     IGDEPMQFHSKFPDVAVAVGEERIVAIPQLLHDRPETRAIILDDAFQHRAVRAGLNVL
                     LTDCSNLFTRDWWLPSGDLRDAPSGYRRADIIVVTKCPEDLSDDQRRAIATEIDPQPH
                     QQIFFSAIRYGTPYHITRQTAGVIDEEVEVLLVTGIANPAPLKRWLNEHSRTYYEMAY
                     SDHHIFSIDDLRAVTRRFEGITAEKKLILTTEKDAVRLIKFRQELEGWPIYVLPIAPK
                     FLFGDEGRFGDVIFQFIKEFK"
     gene            complement(3080..3757)
                     /gene="bioD"
                     /locus_tag="VKQ52_09620"
     CDS             complement(3080..3757)
                     /gene="bioD"
                     /locus_tag="VKQ52_09620"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HLZ87488.1"
                     /translation="MHPLFITGIGTGVGKTLVSAIVTEALDGYYWKPVQAGFAEGTDA
                     EWVGARLSRRVGNGAGSSRVLPGAYTLRMPASPHIAAREEGVVIRVEEIRRQLPAVRP
                     LVVEGAGGLLVPLNDREFMADLALQLDATVILVSRNYLGSINHSLLTAEVCRTRGIRV
                     AGWVFNDQYGDYEEEIVRWSGYPRLGSVPFTGLPDEGFVKGQAERLRANLAYLYDRHP
                     GAVEKPI"
     gene            complement(3759..4307)
                     /locus_tag="VKQ52_09625"
     CDS             complement(3759..4307)
                     /locus_tag="VKQ52_09625"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HLZ87489.1"
                     /translation="MRVIGGELGGRRINPPAKMPHTRPTTDIAKEGLFNILENNLDLS
                     ALRTLDLFGGTGSISYELASRGVKDLTVVEKDPVMFAFIQKTAKELGLEAVYKVVRMD
                     VFKFIGQCGERFDLIFAGPPYALDTIDELPKLVVSRGLLNEGGWFVLEHTPRNNYEGY
                     PLYVTQRHYGTTIFSIFVNKKS"
     gene            complement(4298..5152)
                     /locus_tag="VKQ52_09630"
     CDS             complement(4298..5152)
                     /locus_tag="VKQ52_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HLZ87490.1"
                     /translation="MLKPAFQIEVSDISDEELLQSRLLVEVNPNAFTYVLLNQRNMSP
                     LVVKYFQLEPSKDSPLTDTLREIMEGDELLQRPVKETLLVYNFPESSLIPEPVFTMDT
                     NREIIDTLHGNLQKGLILTEKIPWWELFNVYRISPELHHLLQQTFTAGKYWHYYSLLL
                     KSFKMFDSTEVEDCIKVIFYSDKMVTLVIREGKVQLVQTFLYHDTKDVAYHLLNCCRQ
                     LGLDQESVRLLVGGLIDRQSTLSTELHKYFLRIDFEDIDESIKVTDELKELPLHYFSS
                     ILKMAVCA"
     gene            5315..>6048
                     /locus_tag="VKQ52_09635"
     CDS             5315..>6048
                     /locus_tag="VKQ52_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLZ87491.1"
                     /translation="MDLTNNTKTYKVGITNRDILKMALPISLAILVPQINFITNNIFL
                     GHLDETGNALSTAGITGVYYLLFAVVGQGLNNGLQALIARRAGEGRVEEIGKLFSQGM
                     IIAIVLAFAGILVTWLLAPLALSWSLHSAPLRDQAISFLRIRIWGLPCLYIYQMRNAL
                     LVGTNQSKFLVYGTLAETIVNIALDYGLIFGHFGLPAIGFNGAAYSSIIAEASGMIVV
                     FAVIQAKGISKELQLYKHWVVDRANTR"
BASE COUNT         1317 a         2010 c         1422 g         1299 t
ORIGIN      
        1 ttcctgcgcc tgcgccggcc ccgacgctgc ccgcggttcc atccgtaccg cctccttctc
       61 cgccgccgtg atcacccact cgtaatccag ttgccgcttg gtgagattgg cggctaccac
      121 cttgatagtc accttgtcgc ccatgcggaa ttgccggccg ctacgccggc ccacaagcgc
      181 ataatcggtc tcgacatgcc ggaaatcatc atactccagc aggctgttga gactcaccag
      241 cccctcgcac ttatgctcca ccgtttccac ccagaaccca aaggatgcca ccccgctgat
      301 caccccttcg aactcatccc ccaggaaact ctgcatatac tccacctgct tgtacttgtt
      361 cgccgcccgc tcactctcca tcgccgcccg ctcccgctca ctggattgct tacacttgga
      421 ttccattttt ttatcaacct ctgtctttcc ttccaacacc tcctgcaaca ctctgtgaac
      481 catgacatcg ggataacggc gaatgggcga ggtaaaatga cagtaatgct cgaatcccag
      541 cccgtaatgc ccgatattct ccgtcgtata gaccgccttg gccatcgtcc ggatgcccag
      601 ttgctccagc acatgctgct cgggccggcc ctttacatcc tgcaacatct gattgaatga
      661 cttcgcgatt gcctgcggtg tttgcatgtc gaagacatgc ccgtatttct ttgcgaattc
      721 tacaaagggc agcaactttt ccttgtccgg cgtatcatgc acccggtagg ggaacggaat
      781 gggcttctta ttgaccttga tcttcgccac cgcctccgcc acatggcggt tggccagtaa
      841 catgaactcc tccaccaatt gatgcgattc cttgctctcc tttaccatga tacctaccgg
      901 cttcccgctc ggatcgagct tgaaccgcac ttccgtggag gagaaattga tggccccctt
      961 gcggaaacgg tgcttgcgca gccgctgggc gatatcattc agcagctcga tctccggctg
     1021 ataaagcccg gtcttctgct cgatgatctc ctgcacctcc tcatacgtga agcggtgatc
     1081 ggaatggatc accgtccggc ccagccagga actcttcact ttcgcatccg ccgtcatatg
     1141 gaacaccgcc gagaaggtga gcttgtcctc atgcggccgt aaggaacaga gctcgttcga
     1201 gatccgctcc ggcagcatgg ggttcacccg gtcgggcagg tacaccgagg tcgcccgggt
     1261 aaaggcttcc ttgtccaggg ccgtctccgg ctccacataa taactcacat cggcgatatg
     1321 tatcccgatc tcccattccc ccttatccac ccggcgaagc gaaatggcat cgtcgaaatc
     1381 cttggcatcc accgggtcga tggtaaaggt cagcacctcc cgcatatccc ggcgattggc
     1441 gatctccgcc cccgggataa gatccggcaa ccgcccggac tcctccagca cattctcggg
     1501 gaaaaagatg ggaaacccgt tctccagcag gatatccttc atcgccaggt cgttctggtc
     1561 actcttgtcc atcacctgca cgacctcgcc ctggggcttc ttgttcttct cccattccgt
     1621 gatccggacg atcaccttgt cctggttcac cgccccgttc aggcgggaca aagggacata
     1681 gatatccggc atcggcttat cactctcggc gataaaaaag gcaaaggatt gactgatctc
     1741 gatgcgcccc aggaactcga gctgcttacg atccaccaca tccaccacct ccccctgggc
     1801 gcggccgctc cggcccccgg cactgactac ccgcacccgc acggtgtccc cgtgcaatgc
     1861 ggtgttgaaa tcggccggcc gaaccaggat atcctggtcc cgcccttcca cgatcacaaa
     1921 ccccatccca ctccgcgtaa cgtccaaacg cccctgtaac aggttggcgg agtcggccgg
     1981 ggccttcctt ctgtttttat ctttcttttt gtttttcatt tgaattcttt aataaattga
     2041 aatatcacat ctccaaaccg gccctcgtcc ccaaacagga atttcggcgc gatcggtaat
     2101 acataaatag gccatccctc cagctcctgc cggaatttga taagccgcac cgcatccttc
     2161 tccgtggtca ggatcagctt cttctccgcg gtgatcccct caaaccgccg ggtcacggcc
     2221 cgcagatcat cgatcgaaaa gatatgatga tcgctgtaag ccatctcgta atacgtccgc
     2281 gaatgctcgt tcagccatcg cttcaacggg gcaggattgg caatccccgt aaccagcaac
     2341 acctccacct cctcatcgat aacccccgcc gtctgccggg tgatatgata gggcgtccca
     2401 taccggatcg ccgaaaaaaa gatctgctga tgcggctgcg gatcgatctc cgtcgcaata
     2461 gcccggcgct ggtcatcgga caggtcctcg ggacacttgg tgacgacgat gatatctgcc
     2521 cggcgatagc ccgagggcgc gtcccgcaga tccccgctcg gcagccacca gtcccgggta
     2581 aaaagattgg aacaatccgt cagcaggaca ttgagccccg ccctcaccgc ccggtgctga
     2641 aaagcatcgt cgaggatgat cgcccgcgtc tccggccggt cgtgcagcaa ctgcgggatc
     2701 gcgacgatcc gttcctcccc caccgccacc gccacatcgg ggaatttgct atggaactgc
     2761 atcggctcgt cgccgatatc cagcgccgtg gtcgacgcat tggccagcgc ataccccctt
     2821 gtcttccgct tatacccccg gctcagcacc gcaatggcat accggtcctt caatagccgg
     2881 atcagcaact ccaccatcgg cgatttcccc gttccaccgg cagccaggtt gccgacacag
     2941 ataatgggga ggttaaagga ggtggaagga aagatctttt tatcaaaaca ccagttccgg
     3001 atagtgacga caagtgcgta agcaagcgaa aagggaaata aaaggatgcg tataggcttt
     3061 aacaatggga agttgaaatt catattggtt tttcaacggc accgggatgc ctgtcataaa
     3121 gatacgccaa atttgctctc aaccgctccg cctgcccctt cacgaacccc tcgtccggca
     3181 gaccggtaaa gggtacggac cccaaccggg gatacccgct ccatcgcacg atctcctcct
     3241 cataatcccc atactgatcg ttgaagaccc acccagcgac ccggatcccc cgggtccggc
     3301 agacctccgc cgtcagcaac gagtgattga tactgccaag ataattccgg cttaccagga
     3361 taacggtggc atccagctga agcgcgagat cggccatgaa ctcccggtcg ttcagcggta
     3421 ccagcaatcc cccggcccct tcgaccacca acggccggac agccggcagc tgccgccgga
     3481 tctcctccac ccggatcacg acaccttcct cccgcgcagc aatatgcggc gaggccggca
     3541 tccgcagggt atacgccccg ggcaacaccc gcgacgagcc ggctccattc cccacccgcc
     3601 ggctcaaccg tgccccgacc cactccgcat ccgtcccctc agcgaaaccc gcctgcacgg
     3661 gcttccaata atacccatcc agcgcctcgg tcacgatcgc cgacacaagc gtcttgccga
     3721 cacccgttcc aataccggtt ataaaaagag gatgcatctc agcttttctt gttcacaaaa
     3781 atggagaaga tagtagtacc ataatgccgc tgcgttacat acagcggata gccttcataa
     3841 ttattcctcg gcgtatgctc gaggacgaac cagccgccct cgttcaacaa cccacggctc
     3901 accacgagtt ttggcagctc gtcgatcgtg tcgagagcat agggcggacc ggcaaagatc
     3961 agatcgaacc gctccccgca ctgcccgatg aatttgaaca catccatccg caccacctta
     4021 tagacggctt ccagccccag ctccttcgcc gtcttttgaa taaaggcgaa catgacgggg
     4081 tctttttcca ccaccgtcag gtcctttacc ccacgcgagg ccaactcgta gctgatgctg
     4141 cccgtacccc caaaaagatc gagggtccgc agcgccgata ggtccaggtt attttcgagg
     4201 atgttgaaaa gcccttcctt ggcgatatcg gtcgtcggtc gggtgtgggg catcttcgcc
     4261 ggagggttga tgcgcctgcc tcccaactcc ccaccgatca cgcgcatacg gccattttca
     4321 ggatggaaga aaaataatgc aggggtaatt ccttaagctc atccgtcacc ttgatgctct
     4381 catctatatc ctcgaaatcg atccgcagga aatatttgtg caattccgtg ctcaacgtgg
     4441 attgcctgtc gatcagccca cccaccagca gccgcacgga ctcctggtcg agccccagct
     4501 gccggcagca gttcagcagg tgataggcga catccttggt gtcatggtac aggaaagttt
     4561 gtacgagttg gactttcccc tcccggatca ccagcgtcac catcttgtcg gaataaaaga
     4621 tcaccttgat acaatcctcc acctcggtgg agtcgaacat cttgaaactc tttagcaaca
     4681 ggctgtaata atgccagtac ttgccggccg tgaaggtctg ctgcaggaga tgatgcagtt
     4741 cgggggaaat gcgatagaca ttgaacagct cccaccaggg gatcttttcc gttaggatca
     4801 gccccttctg caggttgcca tgcagggtat cgatgatctc ccggttcgta tccatcgtaa
     4861 agaccggctc ggggatcagg ctgctttccg ggaaattata gaccagcagg gtctccttca
     4921 ccggccgctg caacaactca tccccttcca tgatctcccg cagcgtatcg gtaagcgggc
     4981 tatccttgct gggttccagc tggaaatatt tgaccaccag cggactcata ttccgctggt
     5041 tgagcaatac atacgtaaaa gcattggggt tcacttccac caatagccgc gactgaagca
     5101 attcctcatc ggagatatcc gaaacttcta tctggaacgc aggttttaac atatatgtgc
     5161 aaaatcgggc aatatgaaaa aaatagcccg attttcgcaa acaactaaag tctgcccttc
     5221 gggcgagccg ctgccagcgg ctcttgtgtc agatttgcaa taatactaca aaaacccgta
     5281 aattgccctc ctttttgccg caataccatc ggacatggac cttacgaaca acaccaaaac
     5341 ctataaagtc ggcatcacca acagggacat cctcaaaatg gcccttccca tcagtctcgc
     5401 catcctggtg ccccagatca atttcatcac caacaatatc ttcctcggcc atctcgacga
     5461 aaccggcaac gccctgtcca cagcaggcat caccggtgtc tactacctgc tgttcgccgt
     5521 cgtcggccag ggactcaaca acggactcca ggcccttatc gccagacgcg cgggggaagg
     5581 ccgcgtcgaa gagatcggta aactcttctc ccagggcatg atcatcgcca tcgtactggc
     5641 cttcgccggt atcctcgtga cctggctgct cgccccactg gccctctcct ggtccctcca
     5701 ttccgcaccc ctccgcgatc aggccatctc cttcctccgc atccgcatct ggggactccc
     5761 ctgtctctat atctaccaga tgcgcaacgc cctgctcgtc ggcaccaacc aaagcaaatt
     5821 cctggtctac ggcaccctgg ccgaaacgat cgtcaacatc gccctcgact acggcctcat
     5881 ctttggccac ttcggcctcc ccgccatcgg ctttaacggc gccgcctact cctccatcat
     5941 tgccgaagcc tccggcatga tcgtcgtctt cgccgtgatc caggcaaaag gtatcagtaa
     6001 ggaattacaa ctctacaaac actgggtagt cgaccgggcc aatacccg
//
LOCUS       DATGFG010000517         9336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1176750, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000517 DATGFG010000000
VERSION     DATGFG010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9336
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176750"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VKQ52_09640"
     CDS             complement(<1..423)
                     /locus_tag="VKQ52_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HLZ87492.1"
                     /translation="MKENLFMRAEDDLDFMPIIPLNETDNEGLNEIEIPKELPVLPLR
                     NTVLFPGVVLPITVGRDKSIKAVNDAYKADKLIGVLAQKDSTIEDPTVTDLEDVGTVA
                     RIIKLIKMPDGGTTVILQGKRRFKVGAVTAEDPYFKAQV"
     gene            complement(628..1215)
                     /locus_tag="VKQ52_09645"
     CDS             complement(628..1215)
                     /locus_tag="VKQ52_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HLZ87493.1"
                     /translation="MKRNAFVLSVALVLAMLHTQAQGFHLGIKAGANLFKVDGQSFSQ
                     EFKFGYNVGAFSELNFTPSIGIQPELLFNQTNYRTGTQFSDVYSGGVNNYEGKLNYLS
                     IPVLLSIRPIPLLSILVGPQFGILLNKNEHLTDQAQQAFKSGDFSLVGGAQLNLASIK
                     VGARYVIGLNNINDVDNSNSWKNQGWQVYAGFRIF"
     gene            complement(1317..1970)
                     /locus_tag="VKQ52_09650"
     CDS             complement(1317..1970)
                     /locus_tag="VKQ52_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HLZ87494.1"
                     /translation="MKPSMNKLAFNKWSAPLALLLLLLTASVSHTYAQQSLELGLKAG
                     GNLMKVGGRSFHGKSYPGFSAGVYGKLNFTSKWSLQPEIDWNQTIAKTTDEFNALYGG
                     VSFQQVNLNYVAIPVLLSFKPVPELSILLGPQYAYLVSQTTGLLQYPNDAGKNAFNHN
                     DLSIVFGGQLNLGKVIFGLRYTAGLNNISFSTTDTWRQYGFQAYIGYQLWDLKLRKK"
     gene            complement(2109..3332)
                     /locus_tag="VKQ52_09655"
     CDS             complement(2109..3332)
                     /locus_tag="VKQ52_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLZ87495.1"
                     /translation="MRSRRFVLIVLFIAVLLDIYVYQAVKTITRDLPSQWRAIVEGAY
                     WGGSILGLVTFVIIPYLQLRYTRVNSYLTATILGSYIGKTLTIVFLLVDDLRRLAEWG
                     IAGVQPGSGTGAILRSPWLSWIGIALGAGVFGSLIYGFGNKYKYRVRRVKLAFENLPA
                     SFKGLKIIQLSDIHAGSLANREAVGKGIDLVLKEEPDLILFTGDLVNNVATEMKDYQS
                     IFSRLRAPMGVFSTLGNHDYGDYGWWETPEQKKANLDRLKQIQKEMGWRLLMNEHVVL
                     EKDGGEIALIGIENWSAKARFPKYGRMDLAYPGAESYPFKILMSHDPSHWDAEVRPGY
                     PDIDLMLSGHTHGMQFGIDLPGFRWSPVQFVYKQWAGIYTAGRQHLYVNRGYGFIGYP
                     GRFGMLPEITVIELA"
     gene            3544..4344
                     /locus_tag="VKQ52_09660"
     CDS             3544..4344
                     /locus_tag="VKQ52_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLZ87496.1"
                     /translation="MAKTIGVANQKGGVGKTTSAINLAASFAVLEFKTLLVDADPQAN
                     STTGVGFDLHNVTQSLYDCMVNHIPVKDVILKSEIPHLDVVPSHIDLVGYEIEMINYP
                     NRESVLKGILEPVKDEYDFIIIDCSPSLGLITVNALTASDSVIVPVQTEFFALEGLGK
                     LLNTVKIVQSRLNPQLAIEGILMTMYDGRLRLCNQVVSEVRKHFDEIVFDTIIHRNTK
                     ISEAPSVGKPVILYDALSKGSVNYLNLAKEILQKNNMTRIRQEERILE"
     gene            4357..5277
                     /locus_tag="VKQ52_09665"
     CDS             4357..5277
                     /locus_tag="VKQ52_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HLZ87497.1"
                     /translation="MSTPHKKDKEALGKGIRSLLQSIDADLKTTSGELKSSVVEAVTN
                     MLRIPLEQIETNPRQPRHDFDEVALQELAQSIRLHDIIQPVTVSRLPTGKYRLISGER
                     RFRAAKIAGLKDIPAYVRQADDQQLLELALLENLQREDLNAMEIALSYKRMMEELNYT
                     QEQVAERMGKERSTVANYIRLLKLPPDIQVAVRSNQLSMGHARALINVDTVDKQLYLF
                     SEIKTKGLSVRQTEELVRKLYKENTPVKNSVKPTLPEAFKRIEDNLASHFSTRVKLNH
                     NKKGEGSISIEYYSLQELNKLLDQLGVSVN"
     gene            5288..6040
                     /locus_tag="VKQ52_09670"
     CDS             5288..6040
                     /locus_tag="VKQ52_09670"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HLZ87498.1"
                     /translation="MKMARFFLLAFTLLFLHFLCIGQTVDTVPPSPKGEQLRTPVKLS
                     DTLPRIDSAIKRRRRTDSPYKIDSVARHVHNPRKATLYSTFFPGLGQIYNRKYWKVPI
                     VWAAVGIPAYTYFYNRNWYQKCQFAISILGAYEAAGLPVPDSMLKKVDARLQTFVSNQ
                     DDNNLRSYRNEFRKDQDYSVLFFILFWGLNIVDATVDAHLMSFDVSNELSLHLDQPSG
                     NATVRGPMGSGMGVSLLFDFHKPRYKQLVALP"
     gene            6104..6820
                     /gene="dapB"
                     /locus_tag="VKQ52_09675"
     CDS             6104..6820
                     /gene="dapB"
                     /locus_tag="VKQ52_09675"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HLZ87499.1"
                     /translation="MKIALIGYGKMGRAIEEIAVSRGHTIVLKVSIDNLEDNTIAKIK
                     DADTAIEFTGPESAFDNIIRCLDAGVPVVSGSTGWLGRLEEAKQYCRQQNGTMLYASN
                     FSVGVNIFFEVNKRLAALMAPHPDYDVRLTEVHHTEKKDAPSGTAITLAEQILEKIKR
                     KKEWVNHISDNLDELEILSERMDPAPGTHTIEYSSAIDTITITHTAHNRRGFATGAVL
                     AAEFIAGKKGIYQMSDVLGF"
     gene            6867..7277
                     /locus_tag="VKQ52_09680"
     CDS             6867..7277
                     /locus_tag="VKQ52_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HLZ87500.1"
                     /translation="MLSKKTQYAFQALMYLAEKESDEPVLIAEIARKKKIPLKFLENI
                     LLELKNANILESKKGKGGGYYFAKNPKDVPLATVMRLLDGPISLLPCVSLYFYERCKN
                     CDEKSCGLHTVMSQVRDANLKILEKKTIADLAKG"
     gene            complement(7279..8139)
                     /locus_tag="VKQ52_09685"
     CDS             complement(7279..8139)
                     /locus_tag="VKQ52_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLZ87501.1"
                     /translation="MNTSLDHLSAGQRQQLQKAVHIIVRAVHPEKIILFGVYGAAGGA
                     GLISTLPPALGAFDLLIVTREEDRRSDYELQDIIESRCREEVAVTALVHDITYVNKRV
                     KERQYFFSMVVLEGTLLFDAGRIPLAEAFVPNLEEVRYLAEKDFERWNRQARDFFRSA
                     EFIRGKRGWNTATFLLHQSAEQCYQAILLAFTGYKPTTHNLDKLRRYTNRFSVELALL
                     FPLDNEDEEDLFRQLSSGYVSARYKEEFIISEDEVEVLMERIRRLLDIAERVCSNHFT
                     SLSKRATGGE"
     gene            8270..8647
                     /locus_tag="VKQ52_09690"
     CDS             8270..8647
                     /locus_tag="VKQ52_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ87502.1"
                     /translation="MHYLGKNLRYLRKQLSKTQSEIASLIKKGQTTIGNWENGISEPN
                     LDELLVISNFFDTPLDILVKVDLAEANMAHRAAGTGTAPYDHNADPTPLVREKDDTLS
                     YVLHEIKSLREEMERLHARLPEK"
     gene            complement(8696..9160)
                     /locus_tag="VKQ52_09695"
     CDS             complement(8696..9160)
                     /locus_tag="VKQ52_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87503.1"
                     /translation="MKPKVQAFLYASMVLFLSVMAIGRANAGVNATYDQHPYYLHALS
                     DLRAARWMIQHRPGDWAQTVDEVEAVRRIDAAIGEIKKAAIEDGKNLEDHPPVDERPD
                     HFGRLHAALDFLRKARQDISHDEDNRFAQGLQARAYMHIDGAINAVKKAIHQ"
BASE COUNT         2347 a         2458 c         2378 g         2153 t
ORIGIN      
        1 gacctgcgcc ttgaagtagg gatcttcggc agtgaccgcc ccaaccttga accttcgctt
       61 gccctgcagg ataaccgtag ttccgccatc cggcatcttt atcagtttga tgatcctggc
      121 caccgtgcct acatcttcga gatcggtaac cgtcgggtct tcgatggtac tatccttttg
      181 cgccagcacc ccgatgagtt tatctgcttt ataggcgtcg ttcacggcct tgatgctctt
      241 gtcgcggccc accgtgatcg gcaggaccac accgggaaaa agtaccgtat tgcgtaaggg
      301 gagtaccgga agttctttgg ggatctcgat ctcgttcagg ccttcgttat ctgtttcatt
      361 caagggtatg atgggcatga agtccagatc atcttccgct ctcatgaata aattctcttt
      421 caatttcttc tagtttattt accgaaccgg gtctgagccg ggtccgcttc aatggaattg
      481 gtgacaaatt agcacctgta aaggaaaaat cctactcttt tatcgacgaa ttatacaggg
      541 gacaagattg gtgccataac gagaaaaatg aagaaaccct cctgttttgg cagaagggtt
      601 gtcttattgc gggtaattta cctcaattta gaagatgcga aaacctgcat acacctgcca
      661 tccctggttc ttccaggagt tcgagttgtc cacgtcattg atgttgttca gtccgatgac
      721 ataccgggct cccactttga tgctggcaag gttcagttgg gcgccgccaa caagcgagaa
      781 atctccactc ttaaaggctt gctgcgcctg atcggtcaga tgttcgttct tgttgagcag
      841 gataccgaac tgcggtccca ccaggatact caatagcggg atcggccgga tactgagcag
      901 aacaggaatg ctgagatagt tcagtttacc ttcatagttg ttgaccccgc cactgtaaac
      961 gtcgctgaat tgagtgcctg tgcggtagtt ggtctggttg aacaggagct cgggctgaat
     1021 accgatgctg ggcgtaaagt tgagctccga aaaagcgccg acattgtaac cgaatttgaa
     1081 ttcctgactg aaagactgcc cgtccacctt aaacaggttg gccccggcct taatgcccag
     1141 atgaaaacct tgcgcctggg tatggagcat ggctaacaca agcgctacgg ataatacgaa
     1201 tgcgttcctc ttcatgatgg tttgttttaa attaacggaa tggattgcaa gtactatttc
     1261 aacttatatg ccagtgtttt tagggtaaca atttctacaa gccatcaaaa cggaaattat
     1321 ttttttctga gtttaagatc ccacagctga taaccgatat aggcctggaa tccgtattga
     1381 cgccaggtgt ccgtcgtgct gaaactgata ttgttgagcc ctgccgtata ccggagccca
     1441 aagatcactt tgccgaggtt taactgaccg ccaaaaacga tggagaggtc gttgtgatta
     1501 aaggcattct tacctgcatc gttcgggtat tgcagcagac ccgtggtttg gcttaccagg
     1561 taggcatatt gcggccccag caatatcgac agctcaggca ccggcttaaa agacaataac
     1621 accgggatcg cgacgtaatt gaggttcacc tgctgaaaac tcaccccgcc gtaaagcgcg
     1681 ttgaactcgt ccgttgtctt ggcaatagtc tgattccagt cgatctccgg ttgcagggac
     1741 cacttggaag taaagttcaa ttttccatag acacccgcgc tgaagcccgg ataggatttc
     1801 ccgtgaaagg atcttccacc caccttcatc agattcccac cggccttgag cccgagctcg
     1861 agggattgct gtgcataggt atgggagacg gaagcagtaa gtagtaacag gagtaaggca
     1921 agaggagcgg accatttgtt aaaagccagt ttgttcatgc tcggtttcat tgttgatgct
     1981 gttttttggt tccttataat catgggatga aaatacgctt gaatagttta tcggtttgtt
     2041 aaaatcctta aagaagtcgt taatggatta acagttctct aacactttat gcgcttccat
     2101 caacgcgctt acgccaattc gatgacggtt atctcgggca acatcccgaa acgccccgga
     2161 taaccgatga agccgtatcc cctgttgaca tacagatgtt gccgccccgc ggtatagatg
     2221 cccgcccact gtttgtagac gaattgcacc gggctccacc ggaagccggg gagatcgatg
     2281 ccgaattgca tcccatgggt atggccggac agcatcagat cgatgtccgg gtatcccggc
     2341 cttacctcgg catcccagtg gctggggtcg tggctcatca ggatcttgaa cggataggac
     2401 tccgcccccg gataggccag atccatgcgg ccatatttgg ggaaccgggc cttggcgctc
     2461 cagttctcga taccgatgag cgcgatctct cccccatcct tttccagcac cacatgttcg
     2521 ttcatcagga gtcgccagcc catttctttc tggatctgtt tcaacctgtc caggttcgct
     2581 tttttctgtt cgggcgtttc ccaccagcca tagtctccat agtcatggtt tcccagggtc
     2641 gagaacactc ccatcggggc ccggagccgg ctaaagatgg actggtagtc cttcatttcc
     2701 gttgcgacat tattcaccag gtcgccggtg aacaggataa ggtcgggctc ttccttcagt
     2761 acgaggtcga tccccttgcc cacggcttcc cggttggcga ggctcccggc gtgaatatca
     2821 gacaattgaa tgatcttcaa acctttgaat gaggcgggca ggttttcgaa ggccagcttt
     2881 acccggcgga ccctgtactt gtatttattc ccgaacccat agataaggga accaaaaacg
     2941 cccgcgccga gggcgatccc gatccaactg agccaggggc tgcggaggat ggcgcccgtc
     3001 ccggaacccg gttgaacccc ggcgatcccc cactctgcca ggcggcgcag atcatccacg
     3061 agcaggaaca cgatggtcag ggtcttgccg atatagcttc cgaggatggt cgccgtcaaa
     3121 tagctgttca cccgggtata gcgcagctgc agataaggga tgatcacaaa ggtgacaaga
     3181 ccaaggatcg agccccccca ataagcccct tcgacaatgg cccgccattg cgatggcagg
     3241 tcccgcgtga tcgtttttac cgcctggtaa acatagatat ccagcaatac cgcaataaaa
     3301 aggacgatca ggacgaacct ccggcttcgc atttcgggtc ttattttgcg caaaaatgca
     3361 gcaaacttcc cgaaataagg acttgcggca aaaaatcgtc cgggaattta tgttgaagaa
     3421 tgtatttttg ccatcctgtc agggttaagg gtctgttgcc atggtgctgc gtcccactta
     3481 taccccgggt ggggggaaat aaattttgtc atcttaccga ttggtgacaa tttagcaatt
     3541 attatggcaa aaactattgg tgttgcaaat caaaagggcg gtgtaggtaa aaccacctcg
     3601 gctatcaacc tggcagcgtc ctttgctgtg ctggaattca agaccttgct ggttgatgcc
     3661 gacccacagg caaacagtac caccggggtt gggtttgacc tgcacaacgt gacgcagagt
     3721 ttgtacgatt gcatggtcaa tcatatcccg gtgaaggacg tcatcctgaa gtccgagatc
     3781 ccccatctgg atgtggttcc ctcccatatc gacctcgtgg ggtatgaaat agagatgatc
     3841 aactatccca acagggaaag tgtgctcaag ggtatcctgg agccggtgaa agacgaatat
     3901 gattttatca tcatagactg ctccccctcc ctgggactga tcacggtgaa tgccctgacg
     3961 gcttccgatt cggtgatcgt gccggtacaa accgagttct ttgccctgga aggtcttgga
     4021 aagttgctga acacggtcaa gatcgttcag agccgcctca acccgcaact ggctatcgaa
     4081 ggcatcctga tgacgatgta tgacggacgc ctgcggttat gcaaccaggt ggtcagtgag
     4141 gtccgcaagc atttcgacga gatcgtgttc gacactatta tccaccggaa caccaagatc
     4201 agcgaggcgc ccagcgtggg caagcccgtg atcctttacg acgccctcag caagggttcc
     4261 gtgaattacc tgaatcttgc caaagagatc ctgcaaaaga acaacatgac gaggatcagg
     4321 caggaagaga gaattttaga ataagtaaag aacgacatga gtactcccca taaaaaagat
     4381 aaagaggccc tcggaaaggg catccggtct ctgctgcaga gcatagatgc cgacctgaag
     4441 acgacttccg gagaattgaa gtcctccgtt gtggaagcag tgaccaatat gctgcggatc
     4501 ccgcttgagc agatcgagac caatccccgg cagccccggc atgattttga cgaggtggcc
     4561 ttgcaggaac tcgcgcagtc gatccggctt cacgatatca tccagcccgt aacggtctcc
     4621 agactgccga cgggaaaata ccggcttatc tccggcgaac gccgtttccg tgcggcgaag
     4681 atcgcggggt tgaaagatat ccctgcctat gtgcgccagg ccgatgacca gcaattgctc
     4741 gaactggccc tgctcgagaa cctccaacgg gaagacctca acgccatgga gatcgcgctt
     4801 agctataagc gaatgatgga ggaactcaac tacacgcagg aacaggtggc cgaacggatg
     4861 ggaaaggaac gcagcactgt agccaactat atccgcctgc tgaagcttcc acccgatatc
     4921 caggtagcag tccgcagcaa ccagctgtct atggggcacg cccgcgcact gatcaatgtc
     4981 gacacggtcg acaaacaatt atatctcttc agcgagataa agacaaaagg cctttccgtt
     5041 cgccagacgg aagagctggt acggaagctg tacaaagaga acacccctgt taaaaattcg
     5101 gtaaagccca cgctgcccga agcattcaaa agaattgaag ataacttagc atcgcatttc
     5161 agcacccggg taaaactcaa tcacaacaag aaaggagaag ggagcatttc cattgagtat
     5221 tactcgctcc aggaattgaa caaattgctg gaccaattgg gtgtctctgt aaactaagcc
     5281 ccttttcatg aagatggccc ggttcttttt gttggcgttc accttgctgt tcctgcactt
     5341 cctgtgtatc ggtcagacgg tcgacactgt tccccccagc ccgaaaggtg agcagctcag
     5401 gaccccggta aagctttcgg ataccctgcc ccggatcgat tcggctatca agcgccggcg
     5461 ccgtaccgat tctccctata agatcgattc tgtggcgaga catgtacaca atccccgcaa
     5521 ggcaaccctg tattccactt ttttcccggg actgggacag atctacaacc ggaaatactg
     5581 gaaggttccc atcgtctggg ccgccgtcgg gataccggcc tatacctatt tctacaaccg
     5641 gaactggtac cagaaatgcc agttcgccat ttccatcctc ggcgcctacg aagcagcggg
     5701 gctgcccgtt ccggattcga tgctcaaaaa agtggacgcg cggcttcaga cctttgtaag
     5761 caaccaggac gataataacc ttcgcagcta ccggaatgag ttccgcaagg accaggatta
     5821 ttccgtcctt ttcttcattc tcttctgggg cctgaatatc gtggacgcca ctgtcgacgc
     5881 ccacctgatg agtttcgatg tcagcaatga actcagtctc catctcgacc agccctccgg
     5941 gaatgcaacg gtgcgggggc ccatgggttc cgggatgggg gtcagccttc tcttcgactt
     6001 ccacaaacca cggtacaagc agttggttgc gctgccctga gaaagaaaag ccgggccggt
     6061 tcccacgaaa aataatcgta gctttgcaaa atttgttcgc aatatgaaga tcgcgcttat
     6121 cggttatgga aaaatgggca gggcgataga agagatcgcc gtgtcgagag gacataccat
     6181 cgtcctgaag gtttcgattg acaacctgga agacaacact atcgcaaaga taaaggacgc
     6241 cgataccgcc atcgagttca ccggtcccga aagcgccttt gacaatatca tccgttgcct
     6301 ggacgcgggt gtaccggtgg tttccggttc taccggctgg ctgggccggc tggaagaagc
     6361 aaagcagtac tgccggcaac agaacggcac catgctctat gccagtaatt tcagcgtagg
     6421 cgtcaacatc ttcttcgaag tgaacaaacg gctggctgct ctcatggcgc cgcatccgga
     6481 ttacgatgtg cggctgaccg aggtccatca taccgagaaa aaggatgcac ccagcggaac
     6541 ggcgatcacg ctggccgagc agatactgga aaaaataaag cggaaaaaag aatgggtcaa
     6601 tcatattagt gataacttag acgaattgga gatcctgagc gaacgcatgg acccggcgcc
     6661 tggtacgcat accatcgagt acagctccgc catcgatacg atcaccatca cacacacggc
     6721 acataaccgc cggggctttg cgaccggcgc cgtgctggcc gcggaattca tcgccggcaa
     6781 aaaaggcatc taccagatga gtgatgtgct gggattttga acgccgcatg ggaaaaacga
     6841 taacaaccag gtcaaaagga taaaaaatgc tatccaaaaa aacgcagtat gctttccagg
     6901 cgcttatgta cctggcggaa aaagaaagcg acgaaccggt gctgatcgca gagatcgccc
     6961 ggaaaaagaa gatccccctc aaattcctgg agaatatcct gctggaatta aagaatgcga
     7021 atatcctgga aagtaagaaa ggaaagggtg gtggctatta ttttgccaaa aacccgaagg
     7081 atgtgcccct ggctaccgtg atgcggctgc tggatggacc catttccctg ctgccctgtg
     7141 tcagcctcta tttttacgaa cgttgtaaga actgcgatga gaaaagctgt ggcctgcaca
     7201 cggtgatgag ccaggtcagg gacgccaatc ttaagatcct ggaaaaaaag accatcgccg
     7261 atctggcaaa gggttgagtc attcccctcc cgtggcccgc ttgctcaggc tggtgaaatg
     7321 attagaacat accctctcgg caatatccag caaccggcgg atcctttcca tcaatacttc
     7381 cacctcatct tcggagatga taaactcctc cttgtaacgg gcgctcacat agccggacga
     7441 tagttgccgg aagagatctt cctcgtcttc gttatcgagt ggaaacaata aggcaagctc
     7501 tacggagaac ctgttcgtat agcgcctgag cttgtcgaga ttatgggtcg tgggtttata
     7561 cccggtaaag gcgagcagaa tggcctggta acattgttcc gccgactggt gcagcaggaa
     7621 ggtggccgta ttccatcctc tttttccccg gatgaactcg gcgctgcgaa agaaatcccg
     7681 cgcctggcgg ttccaccttt caaaatcctt ctccgccaga taacgtactt cttccaggtt
     7741 ggggacaaaa gcctcggcaa ggggtatccg cccggcgtcg aataacaatg tcccttcgag
     7801 aacgaccatc gaaaagaaat actgccgttc ctttacccgc ttattcacat aggttatatc
     7861 gtgtacaagg gccgtcaccg ccacctcttc ccggcagcgg ctctcgatga tatcctgcaa
     7921 ctcgtagtcc gagcgccggt cctcctcccg ggtgacgatc agcaggtcaa aggcgccaag
     7981 agcgggcggc aaggtggaga taaggcccgc accaccggcg gcgccataca ccccgaagag
     8041 gatgatcttc tccggatgaa ccgcccggac aatgatatga actgcttttt gcagctgttg
     8101 acgctgcccg gcagacagat ggtcaagcga agtattcata gttttaaaag gttatgtgtt
     8161 agcaataact ttggctatat ttgtaaaggg ttaaaataca aatatcactt aaattgatat
     8221 tttttaccaa aaaagataat aacattttta gaactctatc tttattgcta tgcactattt
     8281 aggaaagaac ctgcggtatc tgcgaaagca attatccaaa acccagtctg agattgcatc
     8341 tcttataaaa aaggggcaga cgacgatcgg taattgggag aacggtatca gcgagcccaa
     8401 tttggatgag ctgctcgtca ttagcaattt ttttgatacg cccctggata tcctcgtcaa
     8461 ggtggacctc gccgaagcca atatggcgca cagggcggcc ggcacgggaa cggctcccta
     8521 cgatcacaat gccgatccca cgccgcttgt ccgcgaaaaa gatgatacct tatcgtatgt
     8581 cctgcacgag atcaaatccc tgcgcgaaga gatggaacgg ctccacgccc gcctccccga
     8641 aaaataaaaa aggcagccgg atggctgcct ttctcatgtg tgttcgacag aatgtttact
     8701 gatgaatagc cttcttgacg gcgttgatcg ccccgtcgat atgcatatag gcacgcgcct
     8761 gcagcccctg tgcaaaacgg ttgtcctcgt catggctgat atcctgacgc gccttgcgca
     8821 ggaagtccag cgcggcatgc aaacggccaa aatgatccgg acgctcgtct accggcggat
     8881 ggtcttcgag attcttaccg tcctcaatag ccgctttttt gatctcgccg atggcggcat
     8941 cgatccgccg gacggcctct acctcatcta ccgtctgcgc ccagtcgccg ggacgatgtt
     9001 ggatcatcca gcgcgccgcc cgaagatcgg acaacgcatg caggtagtaa ggatgctgat
     9061 cataagtggc gttcacaccg gcattggccc ggccgatggc cattacggat aagaacagga
     9121 ccatcgaagc gtacagaaaa gcttggactt tcggtttcat aacgtgattt taagttttaa
     9181 aatggatccg ttcaaataga tagtaaacgc gtgccaaaga ttaaaaccgc attaaagaaa
     9241 ccgggttaaa cggtcgttaa accctgattg acaaataact gctcagagat gtaggttttc
     9301 gataactatc gccgaagctc ctcccccgcc attgca
//
LOCUS       DATGFG010000518         5037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_49446, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000518 DATGFG010000000
VERSION     DATGFG010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49446"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VKQ52_09700"
     CDS             complement(<1..411)
                     /locus_tag="VKQ52_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87504.1"
                     /translation="MFDVLLSIAISFTVTFLAIPAVINVAEMKKLFDMPDARKVHHAP
                     ITPLGGIGIFAGFIFGSLLTLHFNQFADLQYFIAAAFVIFFLGLKDDILVISPVKKFI
                     GQVLAAFIIIYYGNIQIRSMHGFLGLYQLPEMFSL"
     gene            619..1467
                     /locus_tag="VKQ52_09705"
     CDS             619..1467
                     /locus_tag="VKQ52_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HLZ87505.1"
                     /translation="MKSINMADLKAIASQIRRDIVRMVHGSASGHPGGSLGCTEYFTA
                     LYFKVMKHDPNFHMDGLNEDIFFLSNGHISPVFYSALARSGYFDIKELATFRKINSRL
                     QGHPTTHEHLPGIRVASGSLGQGMSVAIGAALSKKLNNDKNIVFSLHGDGELDEGQNW
                     EAIMFAAAKKVDNLISTVDWNGQQIDGTTKSVMDLMNIHAKFDAFGWTTLEMNGNDMD
                     EVIATLDKAKGLVGQGKPIAIMMHTIMGKGVDFMENDHHWHGVAPNDEQLKKALAQLP
                     ETLGDY"
     gene            complement(1494..2717)
                     /locus_tag="VKQ52_09710"
     CDS             complement(1494..2717)
                     /locus_tag="VKQ52_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87506.1"
                     /translation="MNFLFAWRYFRAKKSTNAINIIAWVSMTAIVVGAASLILVLSVF
                     NGFEDLVKSLYTSFYPDLKITPATGRVLTLTAEQLNRLRTVPGVHALSLVAEDKGLLE
                     SNGIPIPAYIKGVDSNFTRVTGVAEKLERGQFVIGTSDHPQVILGSGIANTLGVEAEK
                     ELLPLGVYLFRRGSTDFSADPTASMNHEAMLTSGAFRIQSDFDEHYAITNLEFVKRMI
                     GLQPDEYSDAELSITDPNQAPAVKQAIMQMLGKDYRVQTRYEQNQSLYSVMGTEKWVI
                     YIVLTLILIVAAFNMVGALTMLVWEKQKDIHVLKALGADDGRIQRIFLSEGLLLALMG
                     GVGGIVLAAIVCLLQIRYKLIPLQGGSFLIDYYPVKLVATDFLLVLGTIGIIALLASW
                     LPARKAASQPIALKS"
     gene            complement(2714..3133)
                     /locus_tag="VKQ52_09715"
     CDS             complement(2714..3133)
                     /locus_tag="VKQ52_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HLZ87507.1"
                     /translation="MQEGKRQKQVGALLMQELSDIFQRLGLTMIDGGLVSLTAVKVTP
                     DLLEARIYLSLFQVKDVPAAMKKIEERGWEIKRELAARVKHQLRRIPTLDYFHDDTLE
                     HADRMEDLFRKIRSEKPGNGEEPAGTAGNPDGKEGPK"
     gene            3208..5022
                     /locus_tag="VKQ52_09720"
     CDS             3208..5022
                     /locus_tag="VKQ52_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ87508.1"
                     /translation="MKKFSVILQYLSRYKGKIVVYMIFNILSIVFGLISLGMLSPFLS
                     LLFDQDGSKGQAVTINTNAIGGLKDLLADVVRHSDKSTALLFICLLIITSTILKNLFY
                     YWSSYISSPIRSATITFLRNDVYDKILKLPIGYFTEQRKGDLISRMTNDIYEIESSVV
                     STLEGLIKDPLTIIGYLAYMIYLSPHLSLFLLILLPVTGFLIGRISRTLKRQSKDASQ
                     RLGESLSVLDETLGGIRIIKGFGAESLLRRKFVQINDTLFHIRNKMNARRDLASPLTE
                     TLGIAILTIVLYYGGMLIFRTHGKGFSGGDLFTYISVFALLINPAKNLSTSMFNIQRG
                     AAAMERVEDLLRAPVTIQDNPNAKKLEVFRDRIEFRGVNFSYEDTVILEDINLVIEKG
                     KTIALVGSSGAGKSTLADLVPRFHDVTGGELLIDGINIKDYSLRSVREQLSIVTQEPI
                     LFNDTIAANIALGKPDATQEEIEQAARIANAHTFIIQKEEGYQTNIGDRGSKLSGGER
                     QRLTIARALLKNPPILILDEATSSLDTESERLVQDAINKMMEHRTSIVIAHRLSTIRH
                     ADEIIVLQKGQIVERGTHDELIGRGGFYKKLVEMQEVK"
BASE COUNT         1131 a         1244 c         1454 g         1208 t
ORIGIN      
        1 caggctaaac atttcgggta gttgatataa gccgaggaag ccgtgcatgc tgcggatctg
       61 gatattcccg tagtagatga tgataaatgc cgccaatacc tggccgatga atttctttac
      121 cggggagatc accaggatat cgtcctttag ccccaggaag aagatgacga atgccgcggc
      181 tataaaatac tgaagatcag caaattgatt aaagtgtaag gtaagcaggc tgccaaagat
      241 aaatccggcg aagatgccga tcccgccgag gggggtgatg ggggcatgat ggactttccg
      301 ggcgtcgggc atatcgaaca gcttcttcat ttccgccaca ttgataacgg cagggatggc
      361 aaggaaggta acggtaaaag aaatagctat cgaaagtaga acatcaaaca tcagtctgat
      421 tttgattcgt gaacaaaaat aggaattctt ttttatgctt ctatatacga caaattctta
      481 ttgcaaagat taggcctgtt gttcctgcgg aggttgtagg gtctggcaag ccgtcgtgta
      541 acaagggaga ctaggactga aagataatac aaaagtttaa gacttgcaaa aaaccgttag
      601 atttgtcgcc aggaattaat taaatcaatc aatatggcag acttaaaagc gatcgcttcg
      661 cagatcagaa gagatatcgt cagaatggtt catggctccg caagcggcca tccgggtggt
      721 tcactcggtt gcaccgaata cttcaccgcg ttgtatttca aagtcatgaa gcacgaccca
      781 aactttcata tggacggtct gaacgaagac atcttctttt tatccaatgg tcatatttcc
      841 cccgtattct attcggccct tgcccggtcc ggatatttcg acatcaaaga actggcaacc
      901 ttccgcaaga tcaattcccg gctgcagggg catcctacca cgcatgaaca tctgcccggt
      961 atccgggtgg ccagcggttc gcttggtcag gggatgagtg tcgccatcgg ggcggctctg
     1021 agcaagaaac tcaataacga caagaatatt gtgttctcgt tacatggtga cggagaattg
     1081 gatgaggggc agaactggga agccatcatg ttcgcggctg caaaaaaagt tgacaatctt
     1141 atctctacgg tcgactggaa cggtcagcag atcgacggga cgaccaagtc ggtgatggat
     1201 ctgatgaata tccatgccaa gttcgatgct tttggctgga ccacgttgga gatgaacggc
     1261 aacgacatgg atgaagtgat cgccaccctc gacaaggcga agggtcttgt gggtcagggt
     1321 aaacccatcg ccattatgat gcatacgatc atgggcaagg gggtcgattt tatggagaac
     1381 gaccatcact ggcatggtgt ggcgcccaat gacgagcaat tgaagaaggc gcttgcgcaa
     1441 ttgcctgaaa cgcttggaga ttattaagat agaaggcccc cggaacgggg cctttaactt
     1501 ttcagtgcga tgggctggct ggcggccttg cgtgcgggca gccaggaggc cagcagggcg
     1561 atgatgccga tggtgcccag taccagcagg aaatcggttg ccaccaattt tacgggataa
     1621 tagtcgatga ggaaggagcc tccctgcagg gggatgagct tgtagcggat ctggaggagg
     1681 caaacgatcg cggccagtac gatcccccct accccgccca tcagggccag caggaggcct
     1741 tcgctgagga agatgcgctg gatcctgccg tcgtcggcgc ccagggcctt tagtacgtgg
     1801 atatcctttt gtttttccca gacgagcatg gtgagtgccc ccaccatatt gaaggcggct
     1861 acgatgagaa tgagggtcag gacgatataa ataacccatt tctctgttcc cataacgcta
     1921 tagaggcttt ggttctgttc atagcgggtt tgtacccggt agtccttccc cagcatttgc
     1981 atgatcgcct gtttgacggc aggggcctgg tttggatcgg tgatcgataa ttcggcatcg
     2041 ctgtattcat cgggttgcag cccgatcatc cgtttgacaa attcgagatt ggtgatggcg
     2101 taatgttcgt cgaagtccga ttggatccgg aaggcgccgg aggtgagcat ggcttcgtgg
     2161 ttcatggagg cggtgggatc ggcgctgaag tccgtgctgc cgcggcggaa gaggtacacg
     2221 cccaggggca gcagttcctt ttcggcttcc acgccgaggg tgttggcgat gcccgagccc
     2281 aggatcacct gggggtggtc gcttgtcccg atgacgaatt ggcctctttc cagcttctcc
     2341 gccacgccgg tcacccgggt aaagttgctg tccactcctt ttatataggc ggggatgggg
     2401 atgccgttac tctcgaggag gcccttgtcc tcggccacca gcgacagggc gtgaacgccg
     2461 ggtaccgtcc tgagcctgtt cagctgttcc gcggtgaggg tgaggaccct gcccgtggcc
     2521 ggggtgatct tcagatcggg ataaaagctt gtatagaggg attttacgag atcctcgaag
     2581 ccgttgaaca cgcttaggac gaggatcagg gaggcggcgc ccaccacgat ggccgtcata
     2641 ctcacccagg cgatgatatt gatcgcattg gtcgatttct ttgcgcgaaa ataccgccag
     2701 gcgaagagga agttcatttg gggccctcct ttccgtccgg gttgccggcc gtaccggcag
     2761 gttcctcacc gttcccgggt ttttccgatc ttatcttccg gaagagatct tccatccggt
     2821 cggcgtgttc gagggtatca tcatggaaat agtcgagggt cgggatacgg cggagctggt
     2881 gttttacccg ggctgccagc tcgcgtttga tctcccagcc ccgttcttcg atctttttca
     2941 tggcggcggg gacatccttc acctggaaaa gactgaggta gatgcgtgcc tcgagcagat
     3001 cgggggtgac ttttacggcg gtgagggaga ccaggccccc gtcgatcatg gtcaggccca
     3061 ggcgttggaa gatatcgctc agctcctgca tcagcaaggc tcccacctgc ttttgtcgtt
     3121 ttccttcttg catatccttt aacttttata aatctttgat ttaacgggat tagcgttaaa
     3181 tttgccccca aatttacgga tttcagcatg aagaagttct cggtgatctt acagtacctg
     3241 tccaggtaca aaggcaagat tgtggtgtat atgatcttta acatcctttc tatcgtcttc
     3301 gggctgatct ccctgggtat gcttagtccc tttctatccc tcctgttcga ccaggatggg
     3361 agcaagggtc aggctgtgac catcaatacc aatgcgatcg gcgggttgaa ggacctgttg
     3421 gcggatgttg tccggcattc cgacaagtct acggccctgc tttttatttg tctgctgatc
     3481 atcaccagca cgatcctcaa gaacctgttc tattactggt cgtcgtatat ctcttctccg
     3541 attcgcagcg ccaccatcac ttttttgcgg aacgatgtct atgacaagat cctcaagctg
     3601 cccatcgggt attttaccga gcagcgcaag ggggatctta tctcgcggat gaccaatgat
     3661 atctatgaga tcgagtcttc ggttgtcagc acgctggaag ggcttatcaa ggacccattg
     3721 acgatcatcg gctatctcgc ctatatgatc tatctcagtc cgcatctttc gcttttcctg
     3781 ctgatcctgt tgccagtgac cggttttctt atcggccgga tcagccggac gctgaagcgg
     3841 cagtcgaagg atgcgtcgca gcgcctgggg gaaagtcttt cggtgctcga tgagacgctt
     3901 ggcgggatca ggatcatcaa gggctttggc gcggagtcgt tgttacgccg caagttcgtc
     3961 cagatcaacg acactctttt tcatatccgg aacaagatga atgcgcggcg ggatctggct
     4021 tcgccattga cggagacgct ggggatcgcg atcctcacca tcgtcctgta ttatggcggc
     4081 atgcttatct tccgtacgca tggcaagggt ttctcggggg gagatctctt tacctatatc
     4141 tccgtctttg cgttgctgat caacccggcg aagaacctgt ccacgtcgat gttcaatatc
     4201 cagcggggtg cggcggcgat ggagcgggtg gaggaccttc tccgggcgcc ggtgaccatc
     4261 caggataatc ccaacgcgaa gaagctggaa gtattccgcg acaggatcga gtttcgcggc
     4321 gttaattttt cctatgagga tacggttatc ctggaggata tcaacctcgt gatcgagaag
     4381 ggcaagacga ttgcgcttgt cggcagcagt ggtgcgggta agtcgaccct tgccgacctg
     4441 gtcccccggt ttcatgatgt caccggcggg gaattgctga tcgacgggat caacataaag
     4501 gactattcgc tccgatcggt gagggagcag ctgagtatcg tgacgcagga gcccattctt
     4561 ttcaacgaca cgatcgccgc gaatatcgcg cttgggaaac ccgatgccac gcaggaggag
     4621 atcgagcagg cggccaggat cgccaatgcg catactttca tcatccaaaa ggaagaaggc
     4681 tatcaaacga atattggtga ccgtgggagc aagctcagcg gcggtgagcg gcagcggctt
     4741 accattgcgc gggccctgct caagaaccca ccgatcctta tccttgacga ggctacttct
     4801 tcgcttgaca ccgagagcga gcggctggtg caggatgcca tcaacaagat gatggagcac
     4861 cggacgagca tcgtcatcgc tcaccggctg agcacgatac ggcatgccga tgagatcatc
     4921 gtgttacaaa agggccagat cgtggaacgg ggcacgcatg atgagctgat cggccgtggc
     4981 gggttctaca agaagctggt cgagatgcag gaggtgaaat aattgttttt gcggctg
//
LOCUS       DATGFG010000519         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_700880, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000519 DATGFG010000000
VERSION     DATGFG010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700880"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VKQ52_09725"
     CDS             <1..790
                     /locus_tag="VKQ52_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLZ87509.1"
                     /translation="SASYKLPPSASAEQNKNLTCNFCGAIYQKFVPEYPTPDIAPAIR
                     DNAVIAGYGENVYCPQCLSKNRERLVKAVIEKYGLLPNPASPQAAAPLPAIRILHFSP
                     EKNLYAWLHRQSPVTTVDTAPRFYTSIDPQIRYADATKLPFEDNSFHLVIANHILEHI
                     PEDQKAMREIHRVLTAQGVAILQVPYSTTLTGTIEEPFIDDPKRQAALYGQKDHVRIY
                     ALNDYLRRLKTAGFNVRVLSPEDLIPFRPHAIQPNESVFLCYKH"
     gene            874..1323
                     /locus_tag="VKQ52_09730"
     CDS             874..1323
                     /locus_tag="VKQ52_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLZ87510.1"
                     /translation="MYQSLAQLYLRLALGTAYTFFGLDRLGAWGPYGKPWVSWGDWSH
                     FSTHAHHLMFFLPFGLAEAFAVIATLLEITCGLLLLAGFLTRWAAFTSSGLALCFALA
                     MSIADGITSPLGYSVFTVSAASLLLALQPGYRWSIDSLRTKNPIFTT"
     gene            1320..>2138
                     /locus_tag="VKQ52_09735"
     CDS             1320..>2138
                     /locus_tag="VKQ52_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HLZ87511.1"
                     /translation="MKNITVATAQFEMASGNKNYNLSIIRELSAKAAAAGADAIAFHE
                     CSISGYTFARHLSKQQLLDLAEYIPDGTSIQALTDIARENNIVVLAGLFEKDRENKIY
                     KAYVVVGADGLIAKFRKLHPFINPHLTPGNEYVVFELKGWTCGILICYDNNIIENVRA
                     TTLLGADIIFMPHVTMCTPSTRPGAGFVDPGLWAEKETNPTPLRIEFDGLKGRSWLMK
                     WLPARAYDNAIYAIFSNAIGMDDDQLKNGCSMIVDPFGDVIAECRRLGNDIALAV"
BASE COUNT          487 a          819 c          430 g          402 t
ORIGIN      
        1 gtccgcctcc tacaaactcc ccccgtccgc atcggctgaa caaaacaaaa acctcacctg
       61 caacttctgc ggcgccatct atcaaaaatt cgtccccgaa tacccaaccc ccgatatcgc
      121 acccgccatc cgcgacaacg ccgtcatcgc aggctatggc gagaacgtat actgcccgca
      181 atgcctcagc aagaaccgcg aacgcctcgt aaaagccgtc atcgaaaaat atggcctcct
      241 tcctaacccc gcatcccccc aggctgccgc ccctctcccc gccatccgca tcctccactt
      301 ctcccccgaa aagaacctct atgcctggct tcaccggcaa agccccgtca ccaccgtcga
      361 taccgctccc cgcttctata catccatcga cccgcagatc cggtatgccg acgcaacaaa
      421 actcccgttc gaagacaata gcttccacct cgtcatcgcc aaccacatcc tcgaacacat
      481 ccccgaagac cagaaagcca tgcgcgagat ccaccgcgtc ctcacagcac aaggggtcgc
      541 catcctccag gtgccctatt ccacaaccct gaccggcacg atcgaagagc cattcatcga
      601 cgaccccaaa cgacaggccg ccctctatgg ccagaaagac catgtccgca tctatgcgct
      661 caatgattac ctccgccggc taaagaccgc cggcttcaac gtccgcgtcc tctcccccga
      721 agacctcatc cccttccgtc cccacgccat ccagccaaac gaatccgtct ttctctgcta
      781 caaacactaa catcccctgg cctaccccct ttctccattc tggcacccaa ttatccccat
      841 caataaaaga tctttgcatc aaacaaatac cccatgtacc aatccctcgc acaactctat
      901 cttcggctcg ccctcggcac ggcttacacc ttcttcggcc tcgaccgcct cggcgcctgg
      961 ggcccctatg gaaaaccctg ggtcagctgg ggcgactgga gccatttctc cacccacgcc
     1021 caccatctca tgttcttcct ccccttcgga ctcgccgaag catttgccgt catcgctacc
     1081 cttctcgaaa tcacctgtgg cctcctgctg ctggccggct tcctcacccg gtgggccgca
     1141 ttcaccagca gcggccttgc gctctgtttc gccctcgcca tgtctatcgc cgacggcatc
     1201 acctcccccc tcggttactc cgtattcacc gtcagcgccg ccagtctcct gctggcctta
     1261 cagccgggct accgctggag catcgactcc ctcaggacaa aaaatcctat cttcacaaca
     1321 tgaaaaatat caccgtcgcc acggcacaat tcgaaatggc cagcggcaac aagaactata
     1381 acctttccat catccgtgaa ctctcggcaa aagccgccgc ggcgggagcg gatgccatcg
     1441 ccttccacga atgcagcatc agcggctata ccttcgcccg gcatctgagc aaacaacaac
     1501 tgctcgatct cgccgaatat atccccgacg gcacaagcat ccaggccctc accgacatcg
     1561 cccgcgagaa caatatcgtc gtcctggccg gactcttcga aaaagacagg gagaataaaa
     1621 tatacaaagc ctatgtggtc gtcggcgccg acggcctcat cgccaaattc cgcaagctcc
     1681 atccctttat caacccgcat ctcacccccg gcaacgaata cgtggtcttc gaactgaaag
     1741 gttggacatg cggcatcctc atctgctatg acaacaatat catcgagaat gtaagggcca
     1801 ccaccctgct gggcgcggat atcatcttta tgccgcatgt caccatgtgc accccttcca
     1861 cccgccccgg cgccggcttc gtcgaccccg gactctgggc ggaaaaagag accaacccta
     1921 cccccctccg catcgagttc gacggactga aaggccggtc atggctgatg aaatggctgc
     1981 ccgcacgagc ctacgacaac gccatctacg ccatcttctc caacgccatc ggcatggacg
     2041 acgaccagct gaagaacggc tgctccatga tcgtcgatcc cttcggtgac gtcattgccg
     2101 aatgccgccg gctcggcaac gatatcgccc tggccgtc
//
LOCUS       DATGFG010000520         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_513698, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000520 DATGFG010000000
VERSION     DATGFG010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513698"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VKQ52_09740"
     CDS             <1..725
                     /locus_tag="VKQ52_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ87512.1"
                     /translation="AADGRTAYYASDRADSRGGLDLYSFEMREDIRPAQTTWVRGRVF
                     DRKTNKGLPSSVILTDLSTRDVISKLQTDETGNYLITLPKGKEYAFNVNRRGYLFFSE
                     HFALDKDQGDSAYHIDIPLQPLEANAAVTLKNIFFESNKYELKSESEAELNEVVQLMK
                     ENPTLRIQINGHTDNSGKATENQVLSENRARAVTNYLTTKGIAAVRLSYKGFGDSQPV
                     ADNATPEGRAKNRRTELTVISK"
     gene            769..1899
                     /gene="dprA"
                     /locus_tag="VKQ52_09745"
     CDS             769..1899
                     /gene="dprA"
                     /locus_tag="VKQ52_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLZ87513.1"
                     /translation="MEQQALYQMALTRIPGIGPAYTRKLIARFGDAVAVFQAKKEQLL
                     AMEGIREGPADAILGFAGHKALQAELVLLEKKGVRPLFFTDAGYPDRLRKVPDSPPLL
                     FYKGNADLNARRILAVVGTRNPSDYGRQATAQLIRQLAQPGLLVISGLAAGIDTAAHK
                     AALNQQLPTVGILGHGFGYLYPPENESLANAMLRQGGLLTSFPFDAGPEKYHFPDRNK
                     LVAALCDAVLVVETARKGGSLSTVNEARKYHRRVFAVPGRIMDTRSAGCNWLIRQGMA
                     EMLTSGDQLPAAMGWDWPAGGTGVQASLRLSSSDSEGTEQEELLLHLLKEKDSLSIDE
                     LGSRCGLPPSSLALLLLNLETAGLISALPGKRYKCNPSEIRA"
     gene            1965..3470
                     /gene="guaB"
                     /locus_tag="VKQ52_09750"
     CDS             1965..3470
                     /gene="guaB"
                     /locus_tag="VKQ52_09750"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HLZ87514.1"
                     /translation="MARINSSAGKSASPSKFFGDGLTFDDVLLLPAFSQVLPREVDIR
                     SRLTKAITLNTPMLSAAMDTVTEATLAIALAREGGIGILHKNMSIEKQVEQARKVKRS
                     ESGLIIDPITLHKDATIGDALRLMKENKIGGIPICDNNQVLVGILTNRDLRFEDNKRR
                     KVSEVMTPYEKLITAPEGTDLKKAEKILQKYKIEKLPVVNKQGKLIGLITYRDILQLS
                     SCPNAVKDSYGRLLVGAALGITKDLLDRASALQQIGVDVVTLDSAHGHHIGVINALKS
                     LKKSFKNLQVIAGNIGTASGAKALADAGADAVKVGIGPGSICTTRIVAGAGVPQITAI
                     MEAASALRSRGVPVIADGGIRYTGDMVKALAAGANVVMMGSVFAGVEESPGETIIYEG
                     RKFKQYRGMGSIGAMSQGSSDRYFQDVEDDIKKLVPEGIEGRVAFKGSLSEVVQQYVG
                     GLRAGMGYCGAKDIKTLQQQSQFIRITNAGMKESHAHDVEITKEAPNYSRR"
BASE COUNT          846 a          961 c         1019 g          689 t
ORIGIN      
        1 tcgcggcgga tgggcggacg gcctattacg ccagcgaccg ggccgacagc cggggtggcc
       61 tggacctcta ttccttcgag atgcgggagg atatccgtcc cgcgcagacg acctgggtaa
      121 ggggaagggt cttcgaccgc aagaccaata aagggttgcc ctccagcgtc atcctgaccg
      181 atctttccac ccgggacgtc atcagtaaat tgcagaccga tgaaacgggc aactacctga
      241 tcaccctgcc aaagggaaag gagtatgcct tcaacgtcaa ccggaggggc tatcttttct
      301 tctccgaaca tttcgctctc gacaaggacc agggcgacag cgcttatcac atcgatattc
      361 ccctgcagcc tctcgaagcc aatgcggccg ttaccctgaa gaacatcttc ttcgagtcca
      421 ataaatacga actgaagtcc gagtccgaag ccgagctcaa cgaggtggtg cagctgatga
      481 aagagaatcc cacgttacgg atccagatca atggtcatac ggacaattcg ggcaaggcga
      541 cggaaaacca ggtattgtcg gagaacaggg ccagggcggt gaccaactat ctcacaacca
      601 aaggcatcgc agcggtgcgc ctgtcctata aaggttttgg cgacagtcag cctgtcgcag
      661 acaacgccac tccggaagga cgggcaaaga accggcgcac agaactcact gtcatcagca
      721 agtagcctga aagatttgat ttcgtacctt aggggtaatc gctcccttat ggaacaacaa
      781 gccctttatc aaatggccct gacccggata ccgggtatcg gtcccgccta tacgagaaag
      841 ctcatcgcgc gttttggcga tgctgtcgct gttttccagg caaaaaaaga gcagctcctt
      901 gccatggaag ggataaggga agggcccgcg gatgcgatac tggggttcgc cggacataaa
      961 gccctgcaag ccgaattggt cctgctggaa aaaaagggcg tccggccgct cttctttacg
     1021 gatgcgggat atcctgaccg gcttcgcaag gttccggatt ctccgcccct gctcttctac
     1081 aaaggcaacg ccgatctcaa cgcgagaagg atcctggctg tcgtcggcac gcgcaatccg
     1141 agcgactacg gacggcaggc gacggcgcaa ttgatcaggc aattggcaca acccggtctc
     1201 ctcgttatca gcgggctggc ggccgggatc gatacggcag cccacaaagc ggcactgaac
     1261 cagcagcttc ccacggtggg cattcttggc cacgggtttg gctatttata cccgccggag
     1321 aacgaatcgc ttgcaaatgc gatgctccgg cagggcgggt tgcttacttc atttcccttc
     1381 gatgccgggc cggagaagta tcattttccc gatcggaata aactggtggc agccctctgc
     1441 gatgccgtgc tggtcgtgga gacggcgcga aaaggaggga gtctgtcgac cgtgaatgaa
     1501 gcccgtaaat accatcgtcg ggtctttgcc gttcccggga ggataatgga caccaggagc
     1561 gcgggttgca attggcttat ccggcagggg atggccgaga tgctcacatc gggcgatcag
     1621 ttacccgccg ccatgggttg ggattggccg gcgggcggaa caggcgttca ggcttcgctg
     1681 cggttgtctt catccgacag cgaagggacg gaacaggaag agctgctgtt acatctgctg
     1741 aaagaaaagg acagtctatc catcgacgag cttggcagcc gatgcggctt gcccccctcg
     1801 tcccttgcct tgctgctgct caacctggaa acggcgggtc tgatcagcgc attgcccgga
     1861 aagcgataca aatgcaatcc ttccgagatc cgggcctgac aaggctttca ggggcatgcc
     1921 gacaaaaatt tggaccatac cgctcgtcta ccctatcttt gcacatggca agaataaatt
     1981 cctccgctgg aaagtcagca tccccctcaa aatttttcgg agacggactt acgtttgatg
     2041 atgtactctt gttacccgca ttttctcagg tattgccgcg cgaagtagat atccggagcc
     2101 ggcttaccaa agctatcacc ctcaacaccc ccatgctttc cgccgcgatg gataccgtta
     2161 cggaagcaac cctggcgatc gccctggcga gagagggtgg catcggcatc ctgcataaga
     2221 atatgagcat cgagaagcag gtggagcagg cgcgtaaggt gaaaaggagc gagagcggac
     2281 tgatcatcga ccccataacc ctgcataagg acgcgaccat cggcgatgcg ctgcggttga
     2341 tgaaggagaa taagatcggg ggtattccca tatgtgataa caaccaggtc ctggtgggca
     2401 ttcttaccaa tcgcgacctc cgctttgaag acaataaacg ccggaaggtg agcgaggtaa
     2461 tgacgcctta tgaaaaatta ataaccgccc cggaaggcac cgatctgaaa aaggcggaga
     2521 agatcctcca gaaatacaag atcgaaaaat tacccgttgt caataaacag ggtaaactga
     2581 tcgggctgat cacctatcgc gatatcctgc agctatccag ttgtcccaat gccgtcaagg
     2641 attcctatgg ccggttactg gtcggcgccg cccttgggat caccaaggat ctgctggaca
     2701 gggccagcgc tcttcagcag ataggggtgg atgtggtcac actcgacagc gcccacggtc
     2761 atcatatcgg tgtcatcaat gcattgaaat ccctgaagaa atcttttaag aacctccagg
     2821 tcatcgcggg taacatcggt acggcatccg gggcaaaagc gcttgccgat gcgggtgccg
     2881 atgcggtgaa ggtagggatc ggtccggggt cgatctgtac cacccggatc gtcgcgggcg
     2941 ccggagtacc gcagatcacg gccatcatgg aagccgcttc ggcgctccgt tccaggggcg
     3001 tccccgtcat cgccgatggc ggtatccgtt ataccggcga tatggtaaag gcgcttgcag
     3061 ccggcgccaa tgtcgtgatg atgggtagcg tattcgcggg agtcgaggaa agtcccgggg
     3121 aaacgatcat ctatgagggc cggaaattca agcagtaccg gggcatgggc tccatcggcg
     3181 cgatgtcaca gggaagcagt gaccgctatt tccaggatgt ggaagacgat atcaaaaaat
     3241 tagttccgga aggcatcgag ggccgggtag cctttaaggg cagcctgtcg gaagtcgtcc
     3301 agcaatatgt aggcggtctg cgcgcgggga tgggctattg tggcgcaaag gatataaaga
     3361 ccttacaaca acaatcccaa ttcattcgca ttaccaacgc cgggatgaag gaaagccatg
     3421 cacacgatgt agagatcacg aaggaagccc cgaactatag tcgccggtaa accgctgccg
     3481 cccccccggg gcggcagttc aaaactgatc ccgct
//
LOCUS       DATGFG010000521         9514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1340892, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000521 DATGFG010000000
VERSION     DATGFG010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340892"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VKQ52_09755"
     CDS             <1..1176
                     /locus_tag="VKQ52_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HLZ87515.1"
                     /translation="GFTVIQAVAIAELDGLHTPNANGDLPLLHDDPARPNEKYFAYID
                     TLIDIAAGYHLYIALLPTWGDKVFKDWWGKGPEIFHPDNIFTYGKWIGNRFKNKPNLI
                     WIIGGDRDPRPGSEDVAVWRALAKGITEGVGGADKALMSFHPQPKGTGSSSQWFQSDE
                     WLDLNLLQTGHCRDTEVWETVGDDYRKTPVKPVVNGENIYEEMPVCFNAKELGYANAY
                     DVRKAAYLSVFAGACGHTYGCGPVIFFGERGSNLFADMHGWKEALDLTAANEMKHLRA
                     LMESRPMLDRVPDQSMLASEGSCGPERIQATRGKDYAFVYSAYGRDIIVRAHAISGDR
                     VDAGWFDPRTGKSAFIGTFEGGRQLTFTPPLPVASPVPSQREDWVLILDDAATKRWR"
     gene            complement(1234..3480)
                     /locus_tag="VKQ52_09760"
     CDS             complement(1234..3480)
                     /locus_tag="VKQ52_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87516.1"
                     /translation="MKQYLTLVWLIIGLSPTCAIGQLNNGGLYSLFGVDADTRTNYLK
                     YGIVTGSVTSDDWFAPSGAGNNVIDTSNAAFYKALLQSGNNASFSQRMSQLLYARVNG
                     KLWLDAAYGRDYTAASTAKDSTVFTIAAKNGDDPNNWQGGVASTPAKNDLIDVYAHMR
                     RDGLNIHDSLWFFTGIVAFGNAANSYYDVELYKKAFGFNPSTNSFSSAGTSGGHTEWL
                     FDAAGNITQTGDVIVAVSFFPGSVPTIDVRIWVSQTTYNTWHGGSSAPTYFNFGGAFS
                     STSGTYGYASIVSKAGTTAFGAGIANYSGTPAQDTTYATPWGSSSSSAGYESVYQSEQ
                     LIEVGLNLTRIGIDPALYATLNPCQSFFSEIFFASRSSASFSANLQDFVTPLTFLRQP
                     VMDFAVQGDTLRCNHPTGTITLTDKSTAGYYSWQSIGSGNISGANSDSSQLSITKPGT
                     YVVSASPAEGCPTSQVDTIVVPIDTFPPVATAFAGAGGPNIYLYGGNAAASNYPTPFG
                     GSQGLTWNWSGPQNFSSSQQNPVTDTAWGTYNLTVTEKRNGCTATASVPVLAAMFTTL
                     QTDALQLEGHYTGQDVDLAWKDAVVRPGADYIVERSNGQGGFEPIGDVVASLSDSTDE
                     RATFSFTDNHPMDGNDLYRIKLQSADGSIFYSPTLAIGTGASSLRSVYLAANGSQPPT
                     LVVQSAQDRVGTIIEYDISGRILARRDTPFQSGTNTITLREMPKGRVTVVALFSGDKL
                     VWCQKAFY"
     gene            3662..4711
                     /locus_tag="VKQ52_09765"
     CDS             3662..4711
                     /locus_tag="VKQ52_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87517.1"
                     /translation="MHTLSSGSFLTLVTGLAAGLVLCAAAPCTAQPTPQRTLLALSKT
                     NHTLAIVDPASLKIIATVPVGSDPHEVVASTDGKTAYVSIYGSGSLHELSVIDLVGQK
                     ALPAIDTKPLFGPHGLVFVDGKAWFTAEGSKAVGRYDPATGKLDWAMGTGQDRTHMLY
                     VTADGKQVYTTNVSSGTVSLLVDTLIQPGKMAPPGARPREEWMQIVVPASRGSEGFDV
                     SPDGKELWTAASEDGAITIVDLRARKLATKLDARVTGANRLAFTPDGKRVFVSSLQSG
                     ELTVIDAATRKEVKRLKIGHGAAGILMDPEGARAFVACSADNYVAVVDLKTLEVTGHF
                     DVGGVPDGLAWAVRP"
     gene            4876..5421
                     /locus_tag="VKQ52_09770"
     CDS             4876..5421
                     /locus_tag="VKQ52_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87518.1"
                     /translation="MKKAKFVLVLLFPLFVAHHLSAQTVSSSGDTIRYHVVKGYLSFI
                     IPWVTINKNTTTSEFQSATTIGFPVGINIYYTKHFGFSYEITPSVAWQQSAGKTGTSK
                     TSNLLFDPGPIFRFAHGFNIIPRLAFETQGRYGFTPVFNKVYARTPAVDYWFSVSLPA
                     RFGNNAPASVGASLQIGFTFN"
     gene            complement(5545..6018)
                     /locus_tag="VKQ52_09775"
     CDS             complement(5545..6018)
                     /locus_tag="VKQ52_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87519.1"
                     /translation="MKKITALFALSFLLLTGAAHAATTVPAYAATTAPGVPAAISREF
                     SEKFAKATDIRWETMKGYYKATFSINGKTLFAFYTDRGNLMGVAHNLSPLNLPQPLRE
                     EIKSSYAGYWITDLFTYHNADENGVVITLENADKVIVLKSTGSQVWDLYRSSVKS"
     gene            6231..6482
                     /locus_tag="VKQ52_09780"
     CDS             6231..6482
                     /locus_tag="VKQ52_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87520.1"
                     /translation="MTLEEYEELVLVHLEKARDCGEVERIVARSIAKLRDKHLPSATI
                     AEYVHALRSGLEKLSINDFDPVHWCNIRYAIVCLKNYHL"
     gene            complement(6484..7914)
                     /locus_tag="VKQ52_09785"
     CDS             complement(6484..7914)
                     /locus_tag="VKQ52_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLZ87521.1"
                     /translation="MAKANPKKKKAAAKKPASKKKASPAPKASKAPKASKPSKPSKAS
                     PPRTSHKPAPPPVVNSLSAPAAAGPGGRHFAELKPVSEGPWTDNNSTTFFNSPYYNKN
                     AVTAVPPMQNGSDGVLTLDEIIGADAVNKIRANGKLVFHSVGDTGDTTHTKYEADEMP
                     VAEMMVNDVEDPNQANRAAFLFHLGDIIYQFGDEPSFYYEQFYEPYRTYNAPIFAIPG
                     NHDGMTHKQNETPLQPFLDNFCVSTPSASPDAQGLIRDRMTQPGVYFTLDAPFVSIIG
                     LYSNVLDGYPGGIISNYKGKYPAISNAQLDFLQSELTRLKALRATTNTAILIAVHHPP
                     YASAVSKGGSPMLIDDMDKIFNATGVWPDAVLSGHTHHYERWTRNVNGHEIPYVVAGC
                     GGYNIKPLRTAPTISTTAVAPGSQALRNYLPSYGYLKISVNASRLAIALNSTNQQYGA
                     GADTVLVDLNAHKIIREGKGDPNALT"
     gene            8024..>9514
                     /locus_tag="VKQ52_09790"
     CDS             8024..>9514
                     /locus_tag="VKQ52_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ87522.1"
                     /translation="MKLLVCLFLALCLSMTGMAQDSLSVRDSLKHEPALIEKTMNEVI
                     ITSTRSNRTFQNIPTRVEFINGEELDEKGNMKPGDIRMMLNESTGIQTQQVSATSANS
                     SIRIQGLDGRYTQLLKDGFPLYAGFSGGLGLLQTPPLDLKQVEIIKGSSSTLYGGGAI
                     AGLVNLISKTPTDQRELRFLVNGTSAHGLDVNGFYGQRWGKTGLTFYGGRSSNAAYDP
                     SGSVVFSAIPKFDRYVLNPKLFLYLSQKTKLSFGVNTSFENRIGGDMQYIKGHGDNTH
                     GYYERNKSQRLSTQFSLDHQFNEHSGLTVKNSVSNFNRTITIPGYSFDGKQWSSYSEV
                     AYNTRRKNTEWVAGLNVYTDDFSESPRDSFPRRDYRQNTIGGFIQNTWTIRPWLQTEA
                     GLRGDYVADYGLAVLPRISALFKFTPRLTSRLGGGLGYKTPTIFTEETERIQYQGVLP
                     ISPDSNGLERSYGVNGDINYRTHIGQVSFSVNQLFFYTRINRPLVLD"
BASE COUNT         1998 a         2516 c         2904 g         2096 t
ORIGIN      
        1 ggattcaccg ttatccaggc ggtggcgatc gccgaactgg atggattgca tacgcccaat
       61 gccaacggtg accttccgct tctccatgac gaccctgccc ggcccaatga aaaatacttt
      121 gcctatatcg acacgctgat cgacattgcc gcaggttacc acctgtatat tgcgttgttg
      181 ccgacctggg gcgataaggt gttcaaggat tggtggggga agggccctga gatctttcat
      241 cccgataaca tcttcacata tggcaagtgg atcgggaaca ggtttaagaa taagccgaac
      301 ctcatctgga tcatcggtgg cgacagggac ccgcgcccgg ggtcggaaga tgtggcggta
      361 tggagggccc tggcgaaagg gataactgag ggcgtgggcg gggcggacaa ggccttgatg
      421 agctttcatc cccaacccaa gggaacgggt tcctcctccc aatggtttca gagcgacgag
      481 tggctcgatc tcaacctgct gcagacgggg cattgccggg acaccgaggt gtgggaaacg
      541 gtgggtgatg actatagaaa gaccccggtg aagccggtgg tgaacggcga gaacatctac
      601 gaggagatgc ctgtttgttt caatgcgaaa gagctcggtt atgccaatgc ctatgatgtc
      661 cgcaaggcgg cgtatctcag cgtctttgcg ggcgcctgcg gccataccta tggctgtggc
      721 ccggtgatct tttttgggga acgggggagt aatctgtttg ccgatatgca tggatggaaa
      781 gaggcgctgg atctcaccgc ggccaacgag atgaagcatt tgcgggccct gatggagtcg
      841 aggccgatgc tcgaccgggt cccggatcaa tcgatgctgg caagcgaagg cagttgcgga
      901 ccggaacgga tacaggcgac gcggggaaag gattatgcct ttgtatacag cgcctatgga
      961 agagatatca tcgtcagggc gcacgcgatc tccggggata gggtggatgc gggctggttc
     1021 gatccgcgaa cgggtaagtc ggcgttcatc gggacctttg agggcggcag gcaactgacc
     1081 tttacgccgc cattgcctgt tgcgtcgccg gtgccttcgc aacgggagga ctgggtgctg
     1141 atcctggatg atgcggcgac gaaacgttgg cgctgaccgg gcgccgattg cagccgggaa
     1201 tcgcgtcaga tcgaccaggt tgggccgacc gcgttaataa aaagctttct ggcaccagac
     1261 caatttatca ccggagaaca acgcaaccac cgttacacgg cccttgggca tttcccgcag
     1321 ggtgatcgta ttggtacctg actggaacgg tgtatccctt cgggccagga tgcggccgga
     1381 gatatcgtat tcgatgatcg tccctaccct atcctgcgcg gattggacca ccagggttgg
     1441 cggttgcgat ccgtttgccg ccagatagac cgatcgcagc gaagaagcgc cggtcccgat
     1501 ggcaagggtg ggagaataga aaatgctgcc gtcggcagac tgcagtttta tccggtagag
     1561 atcattgccg tccattggat ggttgtccgt aaaactgaag gtggcgcgtt catctgtgga
     1621 gtccgaaagt gatgcgacga cgtcgccgat gggctcaaag ccgccttgtc cattgctcct
     1681 ttcgacgatg tagtcggcgc ccgggcggac cacggcatcc ttccaggcga ggtctacgtc
     1741 ctggccggtg tagtgtccct ccagttgcaa cgcatcggtt tgcaaggtgg tgaacatagc
     1801 cgcaagaaca ggaacggaag cggtggccgt acagccgttc cttttttcgg tgacggtaag
     1861 gttatacgtt ccccatgccg tgtcggtgac ggggttctgc tgggaagagg agaaattctg
     1921 cgggccggac caattccagg tgaggccctg cgatccaccg aacggggtcg gatagttgct
     1981 ggcggctgcg ttgccaccgt ataaatagat attgggtccg ccggcgcctg cgaaggcggt
     2041 cgctacgggt ggaaaggtat cgatcgggac gacgatggta tccacctggc ttgtgggaca
     2101 gccttcagcc ggagacgccg atactacata ggtaccgggc tttgtaatgc tgagctggct
     2161 gctgtcgctg ttggccccgc tgatgttgcc gctgccgatg gactgccagc tataatagcc
     2221 ggcggtggac ttgtcggtaa gcgtgatcgt tcccgtagga tgattacagc gaagggtatc
     2281 gccctgcacc gcgaaatcca tcaccggctg tcgcaggaag gtgaggggcg tgacaaaatc
     2341 ctggaggtta gccgaaaaag aagcggagga ccgggaggca aaaaagatct cggaaaaaaa
     2401 tgactggcag ggattcaggg tagcgtacag ggccgggtcg atgccgatgc gggtcaaatt
     2461 gaggcccacc tcgatcaact gttccgactg gtataccgat tcgtatcccg ccgagctgct
     2521 ggatgagccc cagggtgtag cataggtggt atcctgcgcc ggcgtgccgg agtagttggc
     2581 gataccggcc ccgaatgcgg tggttcccgc tttggagacg atggacgcat atccatatgt
     2641 tccggaagtg gaactgaagg cgccgccaaa gttaaaatag gtgggtgccg aggagccgcc
     2701 atgccaggtg ttgtacgtgg tctggctgac ccagatacgg acgtcgatgg tcggtacgga
     2761 gccggggaag aaactaacgg ccacgatcac gtcgccggtt tgcgtaatat ttccagccgc
     2821 gtcaaaaagc cattcggtgt gcccgccgct ggttcccgca ctggaaaaac tatttgtcga
     2881 aggattaaag ccgaaggcct ttttgtacaa ttccacatcg taataactat ttgccgcgtt
     2941 accgaacgcg acgatgccgg taaagaacca aagggagtcg tgtatgttga gtccatcccg
     3001 gcgcatatgg gcgtagacgt cgatcaggtc gttcttggcg ggggtcgagg cgacgcctcc
     3061 ctgccagttg ttggggtcgt ccccgttctt tgcggcgatg gtaaagacgg tggaatcctt
     3121 tgccgtggac gctgctgtat agtcccttcc ataggcggca tccagccaga gttttccgtt
     3181 taccctggcg tagagcaact gggacatccg ctggctgaag gaggcgttgt tacccgactg
     3241 taaaagcgct ttataaaagg cggcgttgga ggtatcgatg acattgttgc cggcgcccga
     3301 gggagcgaac cagtcatcac tcgttaccga gccggtgacg ataccatact taaggtaatt
     3361 cgtacgggtg tcggcgtcga caccaaagag agagtaaagg ccgccattgt tcaattgtcc
     3421 gatggcgcat gtgggagata agccgataat cagccacaca agggttaggt actgcttcat
     3481 aagtaacgga tttaaaacct catcatagga tggcttatca gttgcgtcct actctaagta
     3541 gttacgctta ccgtaaaaat acgtatttcc ccccgggttt gcaacagtag ttggagcgga
     3601 cgggttgaga aggctcacaa ttaattgact tatattacac tgtcattaaa aacttttaat
     3661 tatgcatacc ctgtcatcgg gatcctttct gacccttgtc accggcctgg cggccgggct
     3721 tgtcctttgc gccgcggccc cctgtactgc gcaaccgacg ccgcaacgca cgttgctggc
     3781 gctttcgaaa accaatcata cgctggcgat cgtcgacccc gcgtcgttaa agatcattgc
     3841 gaccgtgccc gtcggttcgg acccgcacga ggtggttgca tcgacggacg gaaagaccgc
     3901 ttatgtctcc atttatggca gcggcagcct gcatgaactg agtgtgatcg acctcgtcgg
     3961 gcagaaggct ttgccggcga tagatacgaa acctttattc ggtccgcatg ggctggtctt
     4021 tgtcgatggc aaggcatggt tcacggcgga agggtccaag gctgtgggcc gttatgaccc
     4081 cgccacgggc aagctcgact gggcgatggg gacggggcag gacaggacgc atatgctgta
     4141 tgtgacggcc gacgggaagc aggtctacac cacgaatgtc tcttccggca ccgtcagcct
     4201 tttagtggat acccttatcc agccggggaa gatggcgccg cccggagcga ggccgcgcga
     4261 ggagtggatg cagatcgtag ttcccgcgtc caggggatcg gaggggttcg atgtgtcgcc
     4321 tgacggaaaa gagttgtgga cggcggcgag cgaggatggg gccatcacca tcgtggacct
     4381 gcgggccagg aagctggcga cgaaactgga tgccagggtg acgggcgcca accggcttgc
     4441 gttcacgccc gatggaaaac gggtttttgt ttcgagtctt cagtcgggtg agttaacggt
     4501 tatcgatgcc gcaacccgga aggaggtcaa acggctcaag atcggtcacg gcgcggcggg
     4561 gatcctgatg gacccggagg gcgccagggc ctttgtcgcc tgctcggcgg ataattatgt
     4621 ggcggtggtt gacctcaaaa cattggaggt gactggtcat ttcgatgtcg gcggggtgcc
     4681 tgacgggctt gcgtgggcgg taaggccata gggatggttt gcggtttggc gatggggctg
     4741 cggaaggttg gggcgggaga aagttgccga acggcgggag aaagttgctg gttggcgtta
     4801 tatattatcg tatataacgt attttgccgc cgcatcgttg catccgatgg atgccggctt
     4861 aaccaaccaa aacatatgaa aaaagcaaaa tttgtccttg tactgctgtt cccgttgttt
     4921 gtcgcccatc atctttccgc ccaaaccgtt tcctcgtccg gcgataccat ccggtatcat
     4981 gtcgtcaagg gctatctcag ctttatcatc ccctgggtaa cgatcaacaa gaacaccacc
     5041 acctctgaat ttcaaagcgc taccacgatc gggttcccgg tggggatcaa tatctattac
     5101 accaaacatt ttggtttcag ctatgagatc acgccttccg tagcctggca acagagtgcg
     5161 ggcaagacgg gtacgagcaa gaccagcaac ctgttgttcg atcccggtcc gatatttcgt
     5221 ttcgcgcatg gtttcaacat cattccccgg cttgctttcg aaacgcaggg gaggtatggg
     5281 ttcacgcccg tattcaacaa ggtgtatgcg cggaccccgg ctgtcgatta ctggttctcg
     5341 gtgtcgttgc ctgcccgttt tggcaataat gctcccgcct ctgtcggcgc cagtctgcag
     5401 atcggtttca cctttaatta agatggttgg gcggattagg tggtttggcc ggattgatcg
     5461 cggggggtcg gccggaatca ggggggtggc cggttcaggt ggttcggccg gtttgatcag
     5521 gtgcgtggcg cgatcgcctg gtcatcagct cttgacggag ctgcgataca gatcccagac
     5581 ctggctgccg gtgctcttca ggacgatcac cttgtcggcg ttctccagtg ttatgaccac
     5641 gccattttcg tcggcattgt ggtaagtgaa cagatcggtg atccagtagc cggcatagga
     5701 gcttttgatc tcttcccgca gcggctgggg gaggttgagg ggcgaaaggt tgtgcgccac
     5761 gcccatcagg ttaccccgat cggtgtaaaa ggcgaacagg gtcttgccgt tgatggaaaa
     5821 ggtggctttg taataccctt tcatcgtttc ccatctgatg tcggtggcct ttgcaaattt
     5881 ttcgctgaat tccctgctga tggcagcggg cacgccgggg gcggtggttg cggcgtaggc
     5941 ggggacggtg gttgcggcat gggcggcgcc ggtgagtaag agaaaggaga gggcgaagag
     6001 tgctgttatc tttttcattt ttttgttttt tacgatgatg aatttcgatt gacagcacga
     6061 aattatgccg cgccgcggtg gcgttttggc aaatgagatg aaccgggaaa attctgcggg
     6121 caaggggaga ttttgggcct gggagcccct gaaacccact atcaaccggc agattggcaa
     6181 atagccggat attattgtaa atttactata ataaaacgcg gagggttgcc atgacgctgg
     6241 aagaatacga agagctcgtt cttgttcacc tggagaaagc acgggattgc ggggaagtcg
     6301 agcggatcgt cgcccggtcg atcgcgaaat tgcgcgataa gcaccttccc tccgctacca
     6361 tcgcggagta tgtccatgcc ttacgcagcg gactggaaaa actttccata aatgatttcg
     6421 accccgtaca ctggtgcaat atccggtatg cgatcgtctg tctgaagaat taccatttat
     6481 aaatcatgtc aaagcgttgg gatcgccctt gccttcccgg atgatcttgt gtgcgttgag
     6541 atcgaccagg acggtgtcgg cgccggcgcc atattgttga ttggtcgagt tgagggcaat
     6601 ggccaaccgc gaggcgttga cggaaatttt caggtagccg taggagggca ggtaattacg
     6661 cagcgcctgg ctcccggggg caacggcggt ggtactgatc gtcggggccg tccggagggg
     6721 ttttatatta tatccgccgc agccggcaac gacatatggg atctcgtggc cgttcacgtt
     6781 ccgggtccag cgttcatagt gatgcgtatg cccggagagg acggcatcgg gccagacgcc
     6841 ggtggcgttg aagatcttgt ccatatcgtc gatcagcatc ggagagccgc ccttggatac
     6901 cgcggaggca tagggaggat ggtgaacggc gataaggatg gcggtattgg tagtggcccg
     6961 cagcgccttc aaccgcgtga gttccgactg gagaaagtcg agctgcgcgt tgctgatcgc
     7021 gggatatttc cctttgtagt tggagatgat gccgccggga tagccgtcga ggacattgct
     7081 gtagagcccg atgatggaga cgaacggcgc gtcgagcgta aaataaacac ccggctgggt
     7141 catgcggtcc ctgatgagtc cctgggcgtc gggggaagcg gagggagtgg agacgcagaa
     7201 attgtcgagg aagggctgga ggggtgtttc gttctgctta tgggtcatgc cgtcgtgatt
     7261 gccggggatg gcgaatatcg gggcgttgta ggtgcggtag ggttcataga actgttcata
     7321 gtagaaggag ggttcatcgc caaactggta gatgatatca cccaggtgaa acaagaacgc
     7381 ggcacggttc gcctggttgg ggtcttcgac gtcgttgacc atcatttcgg ccacgggcat
     7441 ttcgtccgct tcgtatttgg tgtgtgtcgt gtcgcctgtg tcgccgacgg aatggaacac
     7501 gagctttcca tttgcccgga tcttgttgac ggcatcggcg ccgatgatct catcgagtgt
     7561 caggacgccg tccgatccat tttgcatcgg tggtaccgcg gtgacggcat ttttgttgta
     7621 gtagggcgaa ttgaagaagg tggtgctgtt attgtctgtc catggtcctt ccgaaacggg
     7681 tttcagttcg gcgaaatgcc ggccgcccgg cccggctgcc gcgggtgcgg aaagcgaatt
     7741 aacgacaggg ggcggggcgg gcttgtgcga tgtgcggggg ggcgaggctt tggaaggctt
     7801 tgagggtttg gatgccttcg gggctttcga tgctttgggg gccggggatg ctttcttttt
     7861 ggaagccggc tttttagctg cggccttctt cttcttcgga tttgcctttg ccatagataa
     7921 gattataaat gttctccaaa attagcggta cgaggaagaa ccgccaacgc gaaaaccatc
     7981 cttttttatc caccaatagg gacgggctgc ttatctttgt cgcatgaagc tgctggtctg
     8041 ccttttcctg gcgctatgcc tgtccatgac cggtatggcc caggatagct tatccgtccg
     8101 tgacagtctt aaacatgaac cggccctcat cgagaaaacc atgaacgagg tgatcatcac
     8161 ctccacccgc agcaatcgca cctttcagaa catccccacc cgcgtcgaat tcatcaatgg
     8221 cgaagaactc gacgaaaagg gcaatatgaa acccggtgac atccgcatga tgctcaacga
     8281 aagcaccggc atccagaccc agcaggtgtc ggccacctcc gccaattcca gcatccgcat
     8341 ccagggcctc gacgggcgct acacccagct cctcaaagac ggcttccccc tctatgccgg
     8401 tttctcgggc ggcctgggtc tgctccagac tccgcccctc gacctcaaac aagtggagat
     8461 catcaagggc tcctcctcca ctctctacgg aggcggcgct attgccggtc tcgtcaacct
     8521 gatctccaaa acacccaccg accagcgtga actccgcttt ctcgtcaacg gcacctccgc
     8581 ccacggcctc gacgtcaacg gcttctacgg gcagcgctgg ggcaagaccg gcctcacctt
     8641 ctatggcggc cgcagcagca atgccgccta cgacccctcc ggcagcgtcg tcttcagcgc
     8701 cataccgaaa ttcgaccgct acgtcctcaa ccccaaactg ttcctctatc tcagtcaaaa
     8761 aaccaaacta agtttcgggg tcaacaccag cttcgaaaac cgcatcggcg gcgacatgca
     8821 gtacatcaag ggccatggcg acaacaccca cggctactat gagcgcaaca aaagccagcg
     8881 cctgtccacc caattcagtc tcgaccacca gttcaacgag cacagcggcc tcaccgtcaa
     8941 aaattccgtc agcaacttca accgcaccat taccatcccc ggctacagct tcgacggcaa
     9001 gcaatggtcc agctattccg aggtggccta caacacccgt cggaaaaata ccgaatgggt
     9061 cgccggcctc aatgtctaca ccgacgactt ttcagaatct ccccgcgaca gcttcccccg
     9121 gcgcgactac cggcaaaaca ccatcggcgg ctttattcaa aacacctgga ccatccgccc
     9181 ctggcttcaa accgaagcgg ggctccgcgg cgactatgta gcagactatg ggctcgccgt
     9241 cctgcccagg atctccgcgc tcttcaagtt caccccccgg ctcacctccc gcctcggcgg
     9301 cggcctggga tataagaccc cgacgatctt caccgaagaa accgaacgca tccaatacca
     9361 gggcgtgctt cccatatccc ctgacagcaa cggcctcgaa cgatcctatg gcgtgaatgg
     9421 ggacatcaac taccgcacac atatcgggca agtcagcttc agcgtcaacc agctcttctt
     9481 ttacacccgg atcaacaggc cccttgtcct tgat
//
LOCUS       DATGFG010000522         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_830101, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000522 DATGFG010000000
VERSION     DATGFG010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830101"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VKQ52_09795"
     CDS             complement(<1..458)
                     /locus_tag="VKQ52_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HLZ87523.1"
                     /translation="MLQLNKGDVFAGNYTLIRLIDTGGFADVWEATYRLTGDNVALKI
                     YPRLDEKGIESLESEYKNLFRLQHTHLVKALHFDKQEGYPFLVMVYYEGGNGSRKIGE
                     YTEAEIGRCLYEMAGALHYLHARNIVHKDIKPNNFLMDGEGHFYLADLGL"
     gene            complement(470..1357)
                     /locus_tag="VKQ52_09800"
     CDS             complement(470..1357)
                     /locus_tag="VKQ52_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87524.1"
                     /translation="MKWGLLFLLFWVCCSRTGFAQYQPATASEMTGFVGQVVQASEKN
                     GDVVIHSECGIFSIRSTHLATFTTRMLYTGHEYTIILFSDQMIPNFKLVLWRHDASNW
                     VRVDSSDQDLAKARHLESKFVGDMEIVKVKPFVAQEYAFQLVSMSGKNETGRYGLVIQ
                     EHETKANAYGSRPEVKSGDHFFKTQGYQWAYLKVDPVTKKNLVDGQWQTNTDEGLFMA
                     NEEKKEFQQLQPTSLASKYTINSVTTPNGITAYNLTHSSGVAATMEVDLVNKTLKIWS
                     SANGRSYIIVYTLVDNYYH"
     gene            complement(1388..1846)
                     /locus_tag="VKQ52_09805"
     CDS             complement(1388..1846)
                     /locus_tag="VKQ52_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87525.1"
                     /translation="MKLLARFFFFLFLFCGAWFCGWGQSMVPIIQQTQSTIGQIEKDG
                     DVVDVALYDYIFKDNNSTFTYEYQFYSSDSYTVRVIGDETRNTNLAIRIYRLVGSNWQ
                     QVKQVVATGKAAASLPFRPPGSERFRIEVSCDLTGGYTSSCFGLIIVRVE"
     gene            complement(1859..>3355)
                     /locus_tag="VKQ52_09810"
     CDS             complement(1859..>3355)
                     /locus_tag="VKQ52_09810"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLZ87526.1"
                     /translation="SSEKAGSMLSSKQTGLTPTQYRAPELFDQRMDHDPIKATDVWAL
                     GASLYEMVTGRPPFGELGGLMQKEGSGMERLPAAYSDQLNRLIEECLAKDAWNRPTAS
                     KLEKRAQYYLETGQWYEPEEWKGKEQQGGDQGSRKTIRRDEREHREEGSGQGAAGGGQ
                     GVYDGGAAGGPGGVGGVGGGAGGVGGGRVAGGAGGTERGDQGYGGGAGVAGGGRGSGG
                     AEGNGGAGVGQRKRPSEAIEKADKNTFRMGVIAVVVAITLIIVIFVMASNARRGTATN
                     DPSNTGDTPDVSKVGVVRDSSYQARDTVAVKPVPVVDGGTSGGGTGGGSTGGSGTDGG
                     AGKGTSGGSSGARDGGSFGVSGGAGDGGGTGGGSTAANYLDIKNPVIDDKPEDNCQPQ
                     LTRIVRNGSVLRIYFFVPSCSSRLTLYGPGDERALFLRQHGVKGNVYPLTAISWTGSE
                     AVVPADGLRIIATFQCPPDDVTLLDVREDRNRLDQGMTFITFNGLHLQ"
BASE COUNT          740 a         1238 c          683 g          694 t
ORIGIN      
        1 ctaagcccca gatcagcgag atagaaatgt ccctccccat ccatcaggaa attattgggc
       61 ttgatatctt tatgtacaat gttacgcgca tgcaggtaat gcagggcccc cgccatctca
      121 tacaggcaac ggccgatctc cgcctccgtg tactccccga tcttccggga cccgttccca
      181 ccctcataat agaccatcac gaggaaagga tacccctcct gcttatcaaa atgcagggcc
      241 ttcacgaggt gcgtatgctg cagccggaaa agattcttgt actcggactc caggctctcg
      301 atccccttct catccagccg cggatagatc ttcaacgcca cattatcccc cgtcagccga
      361 taggtcgcct cccacacatc ggcaaagccc cccgtgtcga tgagccggat cagcgtataa
      421 ttgccggcaa aaacatcccc cttgttcaac tgcagcataa cgtcgtttat taatggtaat
      481 agttatcgac aagcgtatac acgatgatat aacttcgccc attcgcagaa ctccagatct
      541 tcagcgtctt gttcaccaga tccacctcca tcgtcgccgc cacccccgaa ctgtgcgtca
      601 ggttgtaagc cgtgatccca ttcggcgtgg tcaccgaatt gatcgtatac ttcgaagcca
      661 ggctggtggg ctgcaactgc tgaaactcct tcttctcctc gttggccata aacaacccct
      721 cgtcggtatt cgtctgccac tgcccgtcga cgagattctt ctttgtcacc gggtccacct
      781 tcagataggc ccactgatac ccctgcgtct tgaagaaatg atccccgctc ttcacctccg
      841 gccggctgcc ataggcgttc gccttcgtct catgctcctg gatcaccagc ccataccggc
      901 ccgtctcatt cttccccgac atcgacacca gctgaaaggc atactcctgc gccacaaaag
      961 gcttgacctt gacgatctcc atatccccca cgaacttgga ttccaaatgc cgcgccttcg
     1021 ccaggtcctg gtccgacgaa tccacccgca cccagttact cgcatcatgc cgccatagca
     1081 ccagcttgaa gttcgggatc atctggtcgg agaaaagaat tatagtgtat tcgtgaccgg
     1141 tatacagcat cctcgtcgta aaagtagcca gatgcgtgct gcggatcgaa aagatcccgc
     1201 actccgaatg gatcaccaca tccccgttct tctcgctcgc ctgcaccacc tgcccgacaa
     1261 accccgtcat ctcggacgcc gtcgcaggct ggtactgcgc aaatccggtg cgggaacaac
     1321 agacccagaa cagtaagaaa agcaaacccc atttcatttg tatcgattga aaagataaat
     1381 aatactccta ctccacccgt acgatgatca acccaaaaca actcgacgta taccccccgg
     1441 taagatcaca ggacacctcg atccggaaac gctccgatcc cggcgggcga aaaggcagcg
     1501 acgccgccgc cttccccgtc gccaccacct gcttcacctg ctgccagttg ctccccacca
     1561 accggtagat ccggatggca agattggtat tgcgcgtttc gtccccgatc acccgcaccg
     1621 tatacgagtc actcgaatag aactggtatt catacgtgaa cgtggaattg ttatccttga
     1681 aaatatagtc gtacaacgcc acatccacga catccccatc cttctcgatc tgcccgatcg
     1741 tactctgcgt ctgctggatg atcggcacca tactctgtcc ccacccgcaa aaccacgcgc
     1801 cgcaaaacag gaacaggaaa aaaaagaatc tcgctaatag cttcatatcg tcgttttatt
     1861 attgaagatg caatccgtta aaggtgataa aggtcatccc ctggtcgagc ctgttccggt
     1921 cctcccgcac atccagcagc gtgacatcat cgggcggaca ctgaaacgtc gcaatgatgc
     1981 gtaacccatc cgccggcaca acagcttcag accccgtcca ggaaatagcc gtcagcgggt
     2041 acacattacc cttcacccca tgctgccgta gaaaaagcgc ccgctcatcc cccgggccgt
     2101 acaacgtcag cctcgacgaa caggagggta caaaaaaata gatgcgcagc acactcccgt
     2161 tccggacgat ccgcgtcaac tgcggctgac aattatcctc cggcttgtca tcgatcaccg
     2221 ggttctttat atccaggtaa ttagctgccg tcgacccacc gccggtgcca ccgccatcac
     2281 ccgccccacc gctgaccccg aacgaacccc catccctggc ccccgaactg ccgccactcg
     2341 ttcctttccc cgcgccgcca tcggtaccac tacctccggt cgacccaccg ccggtgccac
     2401 cgccactcgt tcccccatct accaccggca ccggcttcac cgcaaccgta tcccgtgcct
     2461 ggtaactgct gtctctcaca acacccacct tcgaaacatc gggcgtatcc cctgtattcg
     2521 acggatcatt cgttgcagtc ccgcgccgcg cattactcgc catgacgaag atcacgatga
     2581 tcagcgtgat cgctaccacc acggcaataa cccccatgcg aaaagtattt ttatcagcct
     2641 tctcaatcgc ctccgaaggc ctcttccgtt gcccgactcc ggcgccgcca ttcccttccg
     2701 cccccccgga tccccgtcca cctccggcga cacccgctcc acccccatat ccctgatcgc
     2761 cccgttcggt cccacccgct ccaccggcaa ccctgccgcc accaactcca cccgctccac
     2821 ctccaactcc accaactcca cccggtccac cggcagctcc cccatcataa acaccctggc
     2881 cacccccggc ggcgccctgc ccactaccct cttcacgatg ctcccgctca tcccgccgga
     2941 tcgtcttccg gctaccctga tcaccaccct gctgttcctt ccccttccat tcctccggct
     3001 cataccactg ccccgtctcc agatagtact gcgcccgctt ctccagcttc gacgctgtag
     3061 gacggttcca cgcatccttt gcaagacatt cctcgatcaa ccggttcagt tgatcactat
     3121 aggccgcagg caaccgctcc atcccggagc cctccttctg catcaaccca cctaactccc
     3181 caaacggcgg ccgccccgtc accatctcat ataaagaggc gcccaacgcc cagacatccg
     3241 tcgcctttat ggggtcatga tccatccgct gatcgaacaa ctccggcgcc cggtactgcg
     3301 tcggcgtcaa acccgtctgc ttcgaagaaa gcatactccc cgccttctca ctgct
//
LOCUS       DATGFG010000523         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_501502, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000523 DATGFG010000000
VERSION     DATGFG010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501502"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VKQ52_09815"
     CDS             <1..1213
                     /locus_tag="VKQ52_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ87527.1"
                     /translation="SSFRPILVLKGRFSASKGGILLRKGLVIGQFAITVALIIGSLVV
                     YRQIRFMNKQELGMNIDQMLIVKPPSLTEWDSTFISRMNSMTNDLARIPGIRGAATSW
                     SAMGGETGRSFNVRRIDQDKTIHFTTRNNGISMGFLELYNIKVIAGRGYTYTDFNPDF
                     NKLHNIIINESATKLLGYATPQDAIGKTILRGQRQWTVIGVINNYHQKSLRYPIEPTM
                     LMPAYGTNSAISIKINTQDLPVTIAAVKKVYDAFFPGNFFDYYFLDARYNEQYANDQL
                     FGKAFAIFSGFAIFIACLGLLGLSLFATAQRTKEIGVRKVLGASVGNIVLLLSRDFIG
                     LVIVAFIIASPLAWLVMHRWLQDFAYRINIEWWIFMIAGLLAVTIALVTISYQAIRAA
                     VANPVRALRSE"
     gene            complement(1247..2239)
                     /locus_tag="VKQ52_09820"
     CDS             complement(1247..2239)
                     /locus_tag="VKQ52_09820"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HLZ87528.1"
                     /translation="MKTKLYTLLLLAIPSVALAGARTLNEAISNGSNWSSTSTWSLGR
                     VPASGDSIVIPAGKTVIFDQSATLNNVYITIGGTLNFNQNNSLALDNVSIVSILSGGM
                     LTATHPTPNELLSVGGVNKYDGKTDNTIAGPALASSSTGTSPTGFTVTPITLPVTFVS
                     FSADRSSDGPVQLTWNTANEIDNSHFEIERSLDGSDWTSVATVAAGNSSLADTYNYND
                     ATAPVAQTWYRIRQVDLDGKYLYSKIVLAGATANAQATIIASGRTVTIFPEKLAGGRL
                     MVRLITPGGQVLQQQSFESASSRIDLSLSTSANGIYLVQVTDGSQWSVVKKVML"
BASE COUNT          591 a          683 c          673 g          575 t
ORIGIN      
        1 gtcctccttc cgccccatcc tggtgttgaa gggcaggttc agcgcctcga aaggcggcat
       61 cctgttgcgc aagggattgg tcatcgggca attcgcgatc acggtagcgt tgatcatcgg
      121 gtcactcgtt gtataccggc agatccggtt tatgaacaaa caggagctgg gaatgaatat
      181 cgaccagatg ctgatcgtaa aaccgccttc gctcaccgaa tgggattcca ccttcatcag
      241 ccggatgaac agcatgacaa acgatctggc ccggataccc ggtatccggg gagcggctac
      301 ttcctggagt gcaatggggg gcgagacggg acggagcttt aatgtccgcc ggatcgacca
      361 ggacaaaacc atccatttca ccacgcgcaa caacggcatc agcatgggct ttctcgaact
      421 gtacaacata aaggtcatcg ccggaagggg ctatacctat accgatttca accctgactt
      481 caacaaactg cacaacatca tcatcaatga aagcgccaca aaattgctgg ggtacgctac
      541 cccacaggac gcgatcggca agaccattct ccgcggtcag cggcaatgga cggtcatcgg
      601 ggtgatcaac aactaccatc agaaatccct gcgttatccg atcgagccca cgatgctcat
      661 gcccgcctat ggcaccaaca gcgccatctc cataaagatc aatacccagg accttccggt
      721 gacgatcgcc gccgtgaaaa aagtgtacga cgcctttttc cccggcaatt tcttcgatta
      781 ctatttcctc gatgcccggt acaatgagca atatgccaac gaccagctct ttggaaaagc
      841 cttcgccatc ttttcgggtt tcgccatctt tatcgcctgc ctgggcttgc tggggctctc
      901 tctcttcgcc actgcacaac gtacgaagga gattggcgtc cgcaaggtac tcggtgcgtc
      961 ggtcggtaat atcgtcctgc tcctgtcgcg cgatttcatc ggcctcgtga tcgttgcctt
     1021 tatcatcgcc tcgcctctcg cctggctggt catgcaccgg tggttgcagg actttgccta
     1081 ccggatcaat attgaatggt ggatcttcat gatcgccggt cttctggccg tcactatcgc
     1141 cctggtgacc atcagctacc aggccatccg cgcggccgtc gcaaaccctg tacgggcgct
     1201 gcgcagcgag taaagggaat tttttttttg aaggggcttt ggacggttat aacattactt
     1261 tctttactac cgaccactga ctgccatccg tcacctgcac gaggtatatc ccgttggcag
     1321 aggtggaaag ggacaggtcg atgcggctcg aggcgctttc aaaggactgc tgttgcagca
     1381 cctgcccacc aggtgtgatc aatcttacca tcagccggcc accggccagc ttttcgggga
     1441 agatggtcac cgttctgccg gaggcgatga tcgtagcctg cgcattggca gtggcaccgg
     1501 ccaggacgat cttgctgtac agatacttac cgtcgagatc cacctgacgg atgcgatacc
     1561 aggtctgcgc aacgggtgcg gtcgcgtcgt tataattgta ggtatcggcc agcgaactgt
     1621 tccccgccgc aacggtggcg acggaagtcc aatccgaacc atcaaggcta cgctctatct
     1681 cgaaatgact gttatcgatt tcattggcgg tattccaggt cagctgtacg ggcccatcgc
     1741 tgctgcggtc agcggaaaag gagacaaatg taacgggcag ggttatggga gtgacagtga
     1801 agccggtagg cgaggtgccg gtagagctgc tggcgagggc gggaccggcg atcgtgttat
     1861 ccgttttgcc atcatatttg ttgacgccgc caacggacag cagctcgttc ggggtagggt
     1921 gggttgcagt cagcatacca ccgctaagga tggagacgat ggatacatta tcgagcgcca
     1981 gactattatt ctgattgaag ttaagggtac cgccgatcgt gatatataca ttatttaaag
     2041 ttgcggattg atcgaatata accgtttttc cggccgggat gacgatcgag tcgccacttg
     2101 ccgggacccg gccaagactc caggtgctgg tgctggacca gttgcttcca ttacttatgg
     2161 cttcgttcag ggtacgggcg cctgcgaggg ctacggaggg gatcgcgagg agcagcagag
     2221 tgtaaagttt agttttcata cctaatcagt tttaacctga tatagaaaag acaaggtatg
     2281 taccaaagac acaacccgtg ccaaagcgcg cggctgaccc caagtagatc actgaaaagc
     2341 aatgaattat aaactcacat aaagaaaacc tccattggct ggaaatttcc cgccgatcgg
     2401 aaaaattcca gggataggaa tatttcctct ttttgggata cacggccaca acaatcgatc
     2461 aggccaaaat caatttaatc atctagaact caacccgccg gaaggcgatt tgactattat
     2521 cc
//
LOCUS       DATGFG010000524         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_186514, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000524 DATGFG010000000
VERSION     DATGFG010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186514"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VKQ52_09825"
     CDS             <1..490
                     /locus_tag="VKQ52_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87529.1"
                     /translation="AYDVHSWLEARHEVLMDYVKEGGNLVVQYNRGNLGRMSTAIGPY
                     NLAISNSRVTDEHARVNFLLPDHPVLNYPNKITDRDFEGWIQERGIYFAGQTDPAYQE
                     VLSMSDPGETEQKGSLVIAHYGKGTFVYTGLVFFRELPAGVPGAYRLMANIIALNQKK
                     GF"
     gene            533..685
                     /locus_tag="VKQ52_09830"
     CDS             533..685
                     /locus_tag="VKQ52_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87530.1"
                     /translation="MHRRRDIAIVTAIVVGLVVGRFIKRATFGLLVGLFIGLVVAMLI
                     ANRNRK"
     gene            801..2528
                     /locus_tag="VKQ52_09835"
     CDS             801..2528
                     /locus_tag="VKQ52_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HLZ87531.1"
                     /translation="MSRPDWIVLALTLLGIIAYGLYKSRTTKDLDGYFLSNRTVPWYM
                     VLLGIMGTQASAVTFLSAPGQAYTDGMRFVQYYFGLPLAMVVLCISFVPVFHRLKVYT
                     AYEYLEQRFDLKTRTLTSFLFLLQRGLSTGISISAPSIILSSLLGWDIVWTNIFMGGL
                     LIIYTVSGGAKAVAYTQQLQMIIIFSGMTIAGYLVVHRLPEGVGLSEILHIGGKAGKL
                     NIITTGFTGKRGGFDWNDRYNLLSGIIGGFFLALSYFGTDQSQVGRYLTARSLNESRL
                     GLLLNGLVKVPMQFLILLLGVMVFAFYQYNHEPVFYNKSQLDLLAHSKYKDSLALVTA
                     RYEAAESQRLITMRNPGTTGWPSAFIDPSLLAERKEALEKEKAGYKATIRRWLNDPAV
                     GGDSNDTNYIFLRFVTDYLPKGLVGLLIAVIFLAAWGSIAATLNSLASCTIIDFHKRF
                     SSRTLPAEKEYRLSQLYTFFWGIFCVAVAQLAYNLGNSLIEAVNVLGSLFYGVILGIF
                     LVAFYRPRVGGWAVFISAVIVEIGVIVLNILSRKGIVPLSFLWFNLVGAAGVVLLSEG
                     LQALMKKKI"
BASE COUNT          556 a          629 c          830 g          717 t
ORIGIN      
        1 ggcttatgat gtccatagct ggcttgaggc caggcatgag gtgttgatgg actatgtgaa
       61 agagggcggc aaccttgtcg tgcaatacaa ccgcggtaac ctgggccgga tgtcgactgc
      121 tatcggtccg tacaacctcg ccatctccaa tagccgggtg acggatgagc atgcgcgggt
      181 gaatttcctg ctacccgatc atccggtact gaattatccc aataagataa ccgaccggga
      241 ttttgaggga tggatacagg agcggggtat ctatttcgcg ggacagaccg atccggccta
      301 ccaggaggtg ttgtcaatga gcgacccggg tgaaacggaa cagaaaggta gcctggtgat
      361 cgcgcattat gggaagggaa ccttcgtcta caccgggctt gttttcttcc gggagttgcc
      421 cgccggggtg ccgggcgctt accggctgat ggctaatatt atcgcactga atcagaagaa
      481 aggtttttag gttgatgcgc gcatgctgaa ccccagggaa atttgaaaaa ttatgcatcg
      541 aagaagggat attgccatcg taacggctat tgtagtaggt cttgtagtag ggcggtttat
      601 caagcgggcc acattcgggt tgttggtagg gctgttcatc gggctcgtcg tcgctatgct
      661 gatcgctaat cgtaatagaa aataaaaatg gacgaggata aggttccggt ttttaagagt
      721 tggagcacct ggtattggct ggtgatcggc ttcctcgtgt tttgcatcgc cgttttttat
      781 ttcctgacca aacactattc atgagccggc ctgactggat cgtattggcg ctgacattgc
      841 ttgggatcat tgcctatgga ttgtacaaaa gtcgtactac caaggacctg gacggatatt
      901 tcctgtccaa ccggacggtg ccctggtaca tggtgttgct ggggatcatg gggacccagg
      961 ccagtgcggt gacctttctg tctgctcccg gtcaggcata tacggatggg atgcggtttg
     1021 tccaatacta ttttgggttg ccgctggcga tggtggtgct ttgcatcagc tttgtgccgg
     1081 tgttccaccg gctaaaggtt tataccgcct atgagtatct ggagcagcga ttcgatctga
     1141 agacccgaac gctcacttct tttcttttcc tgttgcagcg ggggctcagt acggggatca
     1201 gcatctccgc gccttccatc atcctttctt ccctgctggg ctgggacatc gtgtggacga
     1261 atatctttat ggggggattg ctgatcattt atacggtcag tggcggggcg aaggccgttg
     1321 cctatacgca gcaattgcag atgatcatca ttttcagcgg gatgaccatt gcgggttacc
     1381 tggtggtgca ccgcctcccg gaaggggtcg ggctttcgga gatcctgcat attggcggca
     1441 aggccgggaa actgaatata attactacgg gttttacggg caagaggggc ggtttcgact
     1501 ggaacgaccg ctacaatctt ttaagcggga tcatcggcgg attcttcctg gcgctgtctt
     1561 atttcgggac cgaccagtcg caggtggggc gttatctcac ggcgagaagt cttaacgaga
     1621 gccggctggg attgctgctg aacgggcttg tcaaggttcc gatgcaattc ctgatcctgt
     1681 tgctgggggt gatggtcttt gccttttacc agtacaatca tgagccggtg ttctataata
     1741 agtcgcagct ggacctgctg gcgcattcga agtacaagga ttcgctggcc ctggtgaccg
     1801 cccggtatga ggcggcggag agtcagcggc ttatcactat gcgaaatccg ggtactacgg
     1861 gctggccgtc cgcatttatc gatccgtcgc tgctggcgga aaggaaggag gcgcttgaaa
     1921 aggagaaggc gggttataag gcgacgatcc ggcggtggtt gaacgaccct gccgtgggcg
     1981 gcgactccaa cgataccaat tatatctttc tgcgatttgt caccgactat ttgccaaaag
     2041 ggctcgtagg gctgctgatc gccgttatct tcctggctgc gtgggggtcc attgcggcca
     2101 cgctcaattc cctggcctcc tgtaccatca tcgattttca caaacgcttt tcatccagga
     2161 ccctgcccgc ggaaaaggag taccggctct cgcagttgta tacctttttc tggggtatct
     2221 tctgtgtggc ggtggcgcaa ctggcctata atctcggcaa cagtcttatc gaggcggtga
     2281 atgtgctggg gtcgttgttc tatggcgtca tcctggggat cttccttgtg gcgttttatc
     2341 gtccgcgggt aggcgggtgg gcggtgttta tcagcgcggt gatcgtggag atcggggtca
     2401 ttgtgctgaa tatcctgagc cggaagggca ttgtaccgtt gagtttcctc tggtttaacc
     2461 tcgtgggggc tgcgggtgtg gtgctgttga gtgaaggatt gcaggcgttg atgaagaaaa
     2521 aaatatgact ataaacccaa atgccttgca tatatgaaaa gaacgctttt tacgctctgc
     2581 ctgatggccg tctatgtcct ccgggcacag accatcaacg taacggtttc agaaaaattc
     2641 actttgcgtg accggggcat gaccgaaaag gacggcggta ccctgcgaag aggggatttt
     2701 ttttattgtg tggagacggt ttacaagtcg at
//
LOCUS       DATGFG010000525         5613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_29309, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000525 DATGFG010000000
VERSION     DATGFG010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29309"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="VKQ52_09840"
     CDS             complement(<1..1684)
                     /locus_tag="VKQ52_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ87532.1"
                     /translation="MADEIKKRKIFDFSLLRRVFRYAAPYKNRIYWSIALSILLAILS
                     PLRPFLIQLTINTYIRNGWVNAIIWMTVFQIVMLLVETVARFYFSFITAWLGQTVVRD
                     LRVDVYKKVLGLNLSQFDKTPIGTLTTRTINDIEAINDIFSDGLIPIIADLLSILSVL
                     VFMFWADWRLSLVCLAPFPLLILATWLFKESVNKSFIRVRNAVAALNAFVQEHITGMA
                     IVQAFAAEKREEVKFRKINREHRNANINAIFAYSVFFPVVEIILAVSTGLLVWWGATR
                     VMNLPPAEGKELSGKIVSFFLYLNLLFRPLRVIADKFNVLQMGMVASERVFRVLDNDD
                     LIKTQGSFAPARIRGKVEFDKVNFAYTDDRYVLKNISFTMQPGQTVAIVGHTGSGKTS
                     IISLMNRLYQIQQGEIRIDDVRIEDYSLDALRRSIGVVLQDVFLFSGSVVDNITLRNG
                     DIPMERVITAAKLIGMHDFIMRLPGGYHYDVMERGGSLSLGQRQLLSFIRALLYDPSI
                     LILDEATSSVDTESEQLIQEAIDKLIAGRSSIIIAHRLSTIRKASQIIVLDKG"
     gene            complement(1895..4144)
                     /locus_tag="VKQ52_09845"
     CDS             complement(1895..4144)
                     /locus_tag="VKQ52_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87533.1"
                     /translation="MNGSVRSFSALLLTCALLLAGSALRAQPNSYPYKVAQDRMLWHD
                     YVDKEQQRLILLGGNRNDSVVRLTRDEAVNLQVTDALIRQVDELQQQIELDSTLNTNN
                     KKRYLRGLSDVLQKFDNAVQSREIPAAQAPGMIDAFMRAMQLDRKGESIEPVITGSPY
                     ETGKIVVDCFSYPTENSGVKPARLVLTRRFYEMHPSLIFNYLSTHPGVPFEDSLIIIA
                     GHHDIGQLYDFAAAGNDLGYRIRSSKDSLLHMVAMLATSKSGSGRLYFPFLDNLVKGK
                     ITTEDIDKVKDDDLAYYKLLVQTRLDYAARLLPPLKDTPLEMNALTAMLEKKGKQVFV
                     GEINALHTVENPAIRFKILDPLTPEELYYLVVLSEDEIYTSSYLGVYDRIFQRMKAPY
                     GDSLLMQVHGDYFRKFIKMAAAYNKLDHFLGTMDKQNAGTVMKSFVIHLENAGEEEAV
                     DVADSYSSIVEKSPALARFILNEVKWNYDKNVATGDKKGIIIYHLLQTLFESADTTKK
                     VDLSAKLGIPPIYTVDRHSLADDSGRVIQQVYFYGDKDKDGQNSYADFMRIFLPKPKA
                     KPEWKITENPQWTTITSIRGKPVIIYANKPLFGEDDPDAKAQKALSDYLYDRHLKPTI
                     VIHRGHSYHVKYTIEQMPASARIVVLGSCGGYNNLSEVLKISEDAHIISSKQVGTKTV
                     NEPILQAINNTLVAGRNIEWLPMWRELEAGFHKDPIAREKFDDYIPPYKNLGAIFIKA
                     YRKAMDDGE"
     gene            4341..5021
                     /locus_tag="VKQ52_09850"
     CDS             4341..5021
                     /locus_tag="VKQ52_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87534.1"
                     /translation="MESKGKKILIADDEPDILEIIQYNLTKEGYEVITAKDGDEAIGK
                     ARIVRPDLIILDIMMPKKNGVEVCEVLRSQPSFKETLILFLTALSDEGSHIRGLETGA
                     DDYVTKPISPKVLISRVNALFRRVNKEPEDKVLKIENLSIDPAKFEVMVEGKDVTLAK
                     KEFELLYLLASRPGRVFLRNEILNQVWGNEVIVGDRTIDVHIRKIRQKLGLDCITTVK
                     GVGYKFNL"
     gene            5046..>5613
                     /locus_tag="VKQ52_09855"
     CDS             5046..>5613
                     /locus_tag="VKQ52_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HLZ87535.1"
                     /translation="MPSTKNLSPRQLSALTAGLLAFPISVGIYILTGNAIVGAASLLL
                     IFSGSYGLILFTLEKFIYRKIKLIYKLIHQTKATRKEEVYFKYILPQKSIDEVREDVE
                     KWGEKRNEEIELLKKNEAYRKEFLQNLAHEFKTPIFAIQGYVDTLLNGAMEDMAIRKK
                     FLENAAKNVDRLVNLMNDLDEISTLERGE"
BASE COUNT         1305 a         1542 c         1530 g         1236 t
ORIGIN      
        1 cgcccttgtc gaggacgatg atctggctgg ccttgcggat cgtggaaagg cggtgggcga
       61 tgatgatcga acttcgcccg gcgatcagtt tgtcgatggc ttcctggatc agctgttcgg
      121 attcggtatc gacggaagag gtggcctcgt cgaggatgag gatggaagga tcgtagagca
      181 gggcgcggat aaacgacagt agctggcgct ggcccagcga gagcgagccg ccgcgttcca
      241 tgacatcgta atgatagccg cccggaagcc gcataataaa atcgtgcatc ccgatgagct
      301 ttgccgccgt gatgacgcgc tccatcggga tatccccatt gcgaagggtg atattatcca
      361 ccaccgaacc ggagaacagg aatacatcct gcaagacgac gccgatgctc cggcgcaggg
      421 catcgaggct atagtcttcg atccggacat cgtcgatccg gatctcgccc tgctggatct
      481 ggtacagccg gttcatcagg ctgatgatcg acgtcttccc gctgccggta tgcccgacga
      541 tggccaccgt ctggccgggc tgcatcgtaa aggagatatt tttcagcaca tagcggtcat
      601 cggtataggc aaagttcacc ttgtcgaatt cgacctttcc ccttatccgg gcaggcgcga
      661 aactgccctg cgtctttatg agatcatcgt tgtccaggac acggaacacc cgttcactgg
      721 ccaccatgcc catctgcagc acgttgaact tgtccgcgat gacccgcagc ggccggaaca
      781 gcaggttaag gtagagaaag aaggaaacga tcttcccgct cagctccttt ccctccgccg
      841 ggggcaggtt catgacccgc gtggcgcccc accagacgag caggcccgtg gacacggcca
      901 ggatgatctc caccaccggg aagaaaacgg aataggcgaa gatcgcgttg atattggcgt
      961 tacggtgttc gcggttgatc ttcctgaact tcacctcttc ccgtttctcg gcggcaaagg
     1021 cctggacgat cgccatcccg gtgatatgct cctggacaaa ggcgttcagc gcggcaacgg
     1081 cattccgcac ccggataaag gatttgttca ccgattcctt aaacagccag gtggccagga
     1141 tcagcagggg aaagggcgcc aggcagacaa gagagagccg ccagtcggcc cagaacataa
     1201 agaccaacac ggaaaggatg gacaataagt ccgcgatgat ggggatcagc ccgtcggaga
     1261 aaatatcgtt gatcgcctcg atatcgttga tcgtacgggt tgtcagcgtg ccgatcggcg
     1321 ttttatcaaa ctgcgacagg ttgagcccca acaccttctt atacacatca acccggaggt
     1381 cgcggacgac cgtctgcccc agccaggccg tgataaagga aaaatagaag cgggcgaccg
     1441 tctccacgag gagcatgact atctggaaaa cggtcatcca gatgatggcg ttgacccagc
     1501 cattccggat atacgtgttg attgtcaatt ggatcagaaa gggacgaagg ggggacagga
     1561 tcgccagcag gatggataaa gcgatcgacc agtagatcct gttcttgtag ggagccgcat
     1621 aacggaagac ccgccgcaat aagctaaaat caaaaatctt ccttttcttg atctcgtcag
     1681 ccatgtgatt gggcctcttg ctttgatata aaaaaatagc actcggccca atcacctctt
     1741 acgattggcc ttcggccggc ggaccccggg tccgcaacgc ggtaaagata cgaagattgc
     1801 ggccaaccct ctttgccgct catcacccgg atagccccac gcccacaact actccccatc
     1861 atccatcgca actactcccc gtcatccatc gcaactactc cccatcatcc atcgccttgc
     1921 ggtaggcctt tataaagatg gcgcccaggt tcttgtacgg cgggatatag tcgtcgaatt
     1981 tttcgcgggc aatagggtcc ttgtgaaaac cggcctccag ttcgcgccac atcggcagcc
     2041 attcgatgtt cctgccggcg acgagcgtat tgttaatcgc ctggaggatg ggctcgttca
     2101 ccgtcttcgt acccacctgt ttggacgaga tgatatgcgc atcttcgctg atcttcaata
     2161 cttcgctcag gttgttatag ccgccgcagg agcccagcac gacgatccgc gccgaagccg
     2221 gcatctgctc gatggtatac ttgacatgat agctatgccc ccggtggatg acgatcgtcg
     2281 gcttcaggtg acggtcgtag agataatcgc taagcgcttt ctgcgccttt gcatcggggt
     2341 catcctcgcc aaaaaggggc ttgttggcat aaatgatcac cggctttccc cggatggagg
     2401 tgatggtcgt ccattgcggg ttctccgtga tcttccattc gggttttgcc ttcggtttcg
     2461 gcaggaagat gcgcataaag tcggcatagg agttctgtcc gtccttgtcc ttgtcgccat
     2521 agaagtacac ctgctggatg acgcggccgc tatcatccgc cagtgaatgg cgatcgaccg
     2581 tatagatcgg cgggatcccc agtttcgccg agagatccac cttcttcgtc gtatcggcgg
     2641 attcgaaaag ggtttgtagc aggtgataga tgatgatccc ctttttgtca ccggtcgcca
     2701 cattcttgtc gtagttccat ttcacctcgt tcaggatgaa acgcgccagg gcggggctct
     2761 tctccacgat gctgctgtag gagtcggcca catccacggc ttcttcctcg cccgcattct
     2821 cgagatggat gacaaaggat ttcatcaccg tgccggcatt ctgcttgtcc atcgtcccca
     2881 ggaaatggtc cagcttattg taggcggcgg ccatctttat aaacttccga aagtaatccc
     2941 cgtgcacctg catcagcagg ctgtccccat agggggcctt catccgctgg aagatgcggt
     3001 catagacgcc caggtagctg gaggtataga tctcgtcctc gctaagcaca acgagataat
     3061 acagctcttc gggggtcagc ggatcgagta tcttgaagcg gatggcggga ttctccaccg
     3121 tgtgaagcgc attgatctcg ccgacaaaca cctgtttccc ttttttctcc agcatggccg
     3181 tgagcgcatt catttcgagg ggggtatcct ttagcggcgg gagcaaccgg gccgcatagt
     3241 cgagccgggt ttggaccagc aacttgtaat aggccaggtc gtcatccttc accttgtcga
     3301 tatcctcggt ggtgatcttt ccctttacca ggttgtcgag gaaggggaag tacaagcggc
     3361 cgctcccgct tttgctggta gccagcatgg caaccatatg caggagagag tccttgctgc
     3421 tccggatgcg atagcccagg tcattgcccg cggcggcaaa atcatagagc tgcccgatat
     3481 cgtgatgacc cgcgatgatg atgaggctgt cttcgaaggg cacccctgga tgggttgata
     3541 agtaattgaa gatcagcgaa ggatgcattt cgtaaaagcg ccgggtcagc accagccggg
     3601 cgggtttgac cccgctgttc tcggtaggat aactaaaaca gtcgacgacg atcttccccg
     3661 tctcataagg actgccggtg atcaccggct cgatgctctc ccctttccgg tccagttgca
     3721 tggcccgcat aaaggcatcg atcatcccgg gggcctgggc ggccgggatc tccctggact
     3781 ggacagcgtt gtcgaacttc tggagtacat ccgacagacc ccgcaggtac cgtttcttat
     3841 tattggtgtt gagggtggaa tccagttcga tctgctgctg cagttcatcc acctgccgga
     3901 tcagggcgtc cgtcacctgc aggttgacgg cttcgtcccg ggtcaggcgg acgacgctgt
     3961 cattcctgtt accccccaac aggatcagcc gttgctgctc cttatcgaca tagtcatgcc
     4021 agagcatacg atcctgggcc accttgtacg ggtaggaatt gggctgcgcc cgcagtgcgg
     4081 aaccagccag caacagcgca caggtcaaca ataaggcgga aaaagatcgt accgatccgt
     4141 tcataggcaa ccttttggta atatttgcaa aaatactacc aaaaagacta ccagtataat
     4201 tacaatatag ttgaataaaa taaaatatgt ttgtgggaga atcgggatgt gttatttatg
     4261 tattaagaaa ttgtgaactg acttcccaaa ttcccgcgcc gtggtttatt tcgagtaggt
     4321 ttgcgtaaat tttctgatta atggaatcaa aaggcaagaa gatcctgata gccgacgatg
     4381 aacccgatat cctggagatc attcagtaca atttgacaaa agagggatat gaggtgataa
     4441 cggcaaagga cggtgatgag gccatcggga aggccaggat cgtccgtccc gacctgatca
     4501 tcctggatat catgatgcct aaaaagaatg gggtggaggt ctgcgaggtc ctgcggtcgc
     4561 aaccctcttt taaggaaacc ctgatcctct tcctcaccgc cctcagcgac gagggttcgc
     4621 atatccgggg cctggagacc ggcgccgacg attatgtcac aaagcccatc agccccaagg
     4681 tgttgatcag ccgggtaaat gccctgttcc gccgggtgaa taaagagccc gaggacaagg
     4741 tcctgaagat cgaaaaccta agcatcgacc ccgccaaatt cgaggtgatg gtcgaaggaa
     4801 aggacgtgac cctcgccaag aaggaattcg aattattata cctgctcgca tcccgccccg
     4861 gccgcgtgtt cctgcgaaac gagatcctga accaggtgtg gggcaacgag gtgatcgtgg
     4921 gcgatcgcac catcgacgtc catatccgga agatcaggca aaaattagga ctggactgca
     4981 ttacaacagt aaaaggcgtg ggatataagt tcaatctgta gccgattcat taaattcgtg
     5041 gctccatgcc ttccactaaa aacctgtctc ctcggcagct atccgctctc accgccggtc
     5101 tgcttgcctt tcccatctcc gtaggtatct atatcctcac cggcaatgcg atcgtgggcg
     5161 ccgcctccct gctgctgatc ttttcgggca gctatggact catccttttc accctcgaaa
     5221 agttcatcta ccggaagata aagctcatct acaagctgat ccaccagacc aaggccaccc
     5281 ggaaggaaga ggtctatttc aaatatatcc tgccccagaa aagcatcgac gaagtacggg
     5341 aggacgtgga aaaatggggg gagaaacgca atgaagagat cgaattgctg aagaagaacg
     5401 aagcctatcg caaggaattc ctgcagaacc tggcccatga gttcaagacc ccgatcttcg
     5461 ccatccaggg ctatgtggat accctcctga acggggccat ggaagatatg gccatccgga
     5521 aaaaattcct cgagaatgcc gccaagaacg tcgaccggct ggtcaacctg atgaacgacc
     5581 tggatgagat ctccaccctc gaacgcgggg aac
//
LOCUS       DATGFG010000526         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1503015, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000526 DATGFG010000000
VERSION     DATGFG010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503015"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VKQ52_09860"
     CDS             <1..219
                     /locus_tag="VKQ52_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87536.1"
                     /translation="GYNLKSILKTGVFSSARVYASLLNWFGRDKYKGGVNPEAQNTNL
                     TNSTYPLPGDYGAMPLNKSAVMGINFYF"
     gene            246..1787
                     /locus_tag="VKQ52_09865"
     CDS             246..1787
                     /locus_tag="VKQ52_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87537.1"
                     /translation="MKNIYRITGGLSLGLALLAVSCSKQLNQQPPSSITTGQFYSNTN
                     DFTQAVNGAYNQLRAYPDQVLWMGEMRSDNIYATSDGNRDWQAIRNFSPNLTTVGFIN
                     SGWDNNFNGIYAVNSVLDNLKTKGQNVTDPNLRARFAAECHFLRAFYYFQLVRLYGEV
                     PIIDHEMTAAQVAKIKRSPVTDVYTLIISDLNTAIDSLPGSYTTVDIGRATKWAAKGL
                     LGLVYLTRSGAAYQGVDGPTQGTNEWSLAMAQFNDIIGSGQYTFGASYPNIFSYTSEN
                     NPEVLFDVQFMSTNNGADFPSQLTPLGYWGGVGIAGTYGNGYGSCTFDISRGLRASYT
                     GSMGAATDVRDTFNIQWKYPTNTSAPIQTDTSDPFIKKYLNIAKRGTAYNDWPINFIV
                     MRYTDVLMMKAECILHGAPGAQSTVDSIVNLVRHRAGIANISNVDLPTLMEERRREFL
                     GEGIRWNDLVREGMAVTTMNNWIAPDAQSSTISSVIPAFVIYPVPEQEILTAPGLYSQ
                     NPGYQ"
     gene            1799..>2574
                     /locus_tag="VKQ52_09870"
     CDS             1799..>2574
                     /locus_tag="VKQ52_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HLZ87538.1"
                     /translation="MNLSSILLPAALLLARSMVMAQPLDATGPKKAMPDEWIDQDTHH
                     KVVRLSRKPGNNLSFYFHNNPFIGDKMIFSSTDSTGKHLYTVDLQSLALHRVSENGSN
                     AEIVGHHSRSVYYQSHDSVFNTSIETGKTRMVFVFPAGFKGSITTLNADETLLAGGWA
                     SDEQREIAKKYPEKHDFFDRIFDAHLPNMLFTIDVKTGELKKIHGENTWLGHIQFSPT
                     DPDLLMFCHEGPWQKVDRIWTIRMQSGEVRLMHKRTVENE"
BASE COUNT          626 a          846 c          622 g          480 t
ORIGIN      
        1 gggtataatc taaaaagcat cctcaagacc ggcgtcttct ccagcgcccg ggtatatgcc
       61 tctctgctca actggtttgg ccgggataaa tacaagggcg gcgtaaaccc cgaagcgcag
      121 aacaccaacc tgacgaacag cacctacccg ctgccgggcg actacggagc catgccgctc
      181 aacaaatccg ccgtcatggg gatcaatttt tacttctaat gccaaatcaa agtctgatga
      241 aaacaatgaa aaatatatat aggatcactg gcggtctgtc cctgggcctg gccctgctgg
      301 ccgtctcctg cagcaaacag ctcaaccagc aaccgccctc cagcatcacc accgggcagt
      361 tctacagcaa taccaacgat ttcacccagg ccgtcaacgg cgcctataac cagctccgcg
      421 cctatcccga ccaggtactc tggatggggg agatgcgctc ggataatatc tatgccacct
      481 ccgacggcaa ccgcgactgg caggcgatcc gcaatttctc gccgaacctg accaccgtgg
      541 gctttatcaa cagcggctgg gacaacaatt tcaatggcat ctacgcagtc aacagcgttc
      601 tcgacaacct gaagacaaaa ggccagaacg tcacggatcc caacctgcgc gcccgcttcg
      661 ccgccgaatg ccatttcctg cgggcctttt actacttcca attggtccgc ctctatggag
      721 aagtacccat catcgatcac gaaatgacgg ccgcccaggt ggcgaagatc aaacgcagcc
      781 ccgtgacgga tgtctatact ctcatcatct ccgaccttaa caccgccatc gacagcctgc
      841 ccggcagcta caccaccgtg gacatcggcc gggctaccaa atgggccgca aaaggcctgc
      901 tcggactcgt ctatctgacc cggtcgggcg ccgcctacca gggcgtggac ggtcctaccc
      961 agggcaccaa cgaatggagc ctggcgatgg cccagttcaa cgatatcatc ggcagcggtc
     1021 agtacacctt cggggcaagc tatcccaata tcttctccta taccagcgag aacaacccgg
     1081 aagtgctctt cgatgtgcag ttcatgagca ccaacaacgg cgcggatttc cccagtcaac
     1141 tgacacccct cggctactgg ggtggggtag gcatcgccgg aacatatggc aacggctatg
     1201 gttcctgcac cttcgatatc tccagggggc tccgtgcttc ctataccggt agcatgggcg
     1261 ccgcaacaga tgtccgcgac acctttaaca tccagtggaa gtatccgacc aatacctccg
     1321 cgcccatcca gaccgatacc tccgacccct tcatcaaaaa atacctcaac atcgccaaac
     1381 gcggcaccgc ctacaatgac tggcccatca acttcatcgt gatgcgctac accgacgtgc
     1441 tgatgatgaa ggccgaatgc atcctccatg gcgcccccgg cgcccagtcc accgtggaca
     1501 gtatcgtcaa tctagtaaga caccgcgccg gcatcgccaa catctccaat gtcgacctgc
     1561 cgaccctcat ggaagagcga cgccgcgaat tcctcgggga aggcatccgc tggaacgacc
     1621 tggttcgcga aggcatggcg gtgacgacca tgaacaactg gatcgccccc gatgcacagt
     1681 cctcgacgat cagctccgtc atcccggcat tcgtgatcta ccccgttccc gaacaggaga
     1741 tcctcacggc cccgggatta tattcccaaa atccaggtta tcaataaaaa tagtacgcat
     1801 gaacctatct tccatcctcc tgcccgctgc cctgctcctg gcccgctcaa tggttatggc
     1861 acagccgctc gatgcgacag gcccgaagaa agcgatgccc gatgaatgga tcgaccagga
     1921 cacgcaccac aaagtggtgc gcctgtccag gaaaccgggc aataatctca gtttctattt
     1981 tcacaacaac cccttcatcg gggacaagat gatcttttcc tccaccgaca gtacgggtaa
     2041 acatctttat accgtcgatc tgcagtccct ggcgcttcac cgcgtcagcg agaacggcag
     2101 caatgccgag atcgtggggc atcacagcag gagcgtctac tatcagtccc atgacagcgt
     2161 cttcaacacc agcatcgaaa ccggcaagac ccggatggtc tttgtcttcc ccgccggttt
     2221 caaggggagc atcacgaccc tcaacgccga cgagacattg ctggcaggcg gctgggccag
     2281 cgatgaacaa cgggagattg caaaaaaata cccggagaaa cacgatttct tcgatcgcat
     2341 cttcgacgcc catctcccta atatgctctt caccatcgac gtgaagaccg gggagttgaa
     2401 aaagatccac ggcgagaaca cctggctcgg ccacatccag ttctccccca ccgaccccga
     2461 tctgctcatg ttctgccatg aaggcccctg gcaaaaggtg gaccgtatct ggaccatccg
     2521 catgcagtcc ggcgaggtcc ggctgatgca caaacgcacc gtcgagaacg agat
//
LOCUS       DATGFG010000527         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1195013, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000527 DATGFG010000000
VERSION     DATGFG010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195013"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VKQ52_09875"
     CDS             <1..911
                     /locus_tag="VKQ52_09875"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ87539.1"
                     /translation="NHWIQWLTYTIIFCTGVIRAFAGPALQAIIAQIVPREKLPGAVT
                     LNTSAFLFASVLGHATAGFLIAHTNYLICFSVVGLYVAIAWFFLFTLAPKQIMVAGEK
                     KTWESIKEGLSFVFHTKEVLGALALDLFAVLFGGAVAMIPVYARDILNVGPIGFGWLN
                     AADDIGSIFMIMMMTLFPLRHKQGYILLYAVFGFGVCIIVFGLSKLYLLSFIALLCSG
                     MLDGISVVIRGTIVQVKTPDPMRGRVSSVNSMFINSSNELGQFESGVMARLLGVVPSV
                     VFGGCMTILVVITTWFRAPTLRKLEY"
     gene            complement(972..1466)
                     /locus_tag="VKQ52_09880"
     CDS             complement(972..1466)
                     /locus_tag="VKQ52_09880"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HLZ87540.1"
                     /translation="MSDLLNNWEKKSKDRQKDYRRFLEKAEKPAVRNRVLEQLPRLHE
                     EAFERVDCLQCANCCKHYSPRFKTPDIKRIAKHLKLKESDFIGRYLRLDADGDYVVKT
                     TPCPFLGADNYCGIYEVRPSDCERFPYTDEDVLVRRPALTQKNSTFCPIVYFVLERLI
                     TSLP"
     gene            1658..2551
                     /locus_tag="VKQ52_09885"
     CDS             1658..2551
                     /locus_tag="VKQ52_09885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HLZ87541.1"
                     /translation="MISTPIYLIIIGLEILLSNYRHRKTYTFKDTLSNLYLMLLNSGI
                     DLLFRGIYLVVLQFCYRHHWISITNVFSYWLLLLLAEDFLYYWLHRWDHEIRFFWAVH
                     VTHHSSEHMNFTVGFRSSVFQPLYRWVYFIPLALVGFKPLDIVFVYSATQIWGIFVHT
                     ELIGKMGWLEYILVTPSHHRVHHASNPRYLDKNMGMFLIIWDQLFGTFQPELTPEEYQ
                     PTRYGLTKPVEKPGPSRLVFHEWRAIGKDLARRDIGWKEKWQYVFGPPGWSHDGSRQT
                     SEQMREAEAVENLDFAPDPQF"
     gene            2959..>3284
                     /locus_tag="VKQ52_09890"
     CDS             2959..>3284
                     /locus_tag="VKQ52_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87542.1"
                     /translation="MNLFEQQANEFAGRHIGPNEKETSGMLRTIGAGSLDELIDRTIP
                     ASIRSRKSLNIPGPLSEHQYLTSLRQIAGLNKVFKSYIGQGYYDTIVPSVILRNVFEN
                     PGWYTQ"
BASE COUNT          696 a          921 c          850 g          817 t
ORIGIN      
        1 ggaaccactg gatccagtgg ctgacctata cgatcatctt ctgcaccggg gtgatccgcg
       61 cctttgcggg tcccgcactc caggccatca tcgcccagat cgtgccaagg gaaaaactgc
      121 ccggcgccgt cacgctcaac accagcgcct ttttgtttgc ctcggtactc gggcacgcca
      181 cggcggggtt tctcatcgcg cataccaatt atctcatttg cttcagcgtc gtcgggctgt
      241 atgtcgccat cgcctggttc ttccttttta cgctcgcacc caaacagatc atggtggccg
      301 gcgaaaagaa gacctgggaa agcataaagg aagggctcag ttttgtcttc catacaaaag
      361 aagtgctggg ggcgctggcg ctcgatctct ttgcggtgct tttcggcggc gccgtggcca
      421 tgatccccgt ctatgcccgc gatatcctga acgttggccc catcggtttc ggctggctca
      481 acgctgccga cgatatcggc tccatcttca tgatcatgat gatgaccctt ttcccgctgc
      541 gccacaaaca ggggtatatc ctcctctatg ccgtattcgg tttcggggtt tgcatcatcg
      601 tgttcgggct ttcgaaacta tacctgctct cctttatcgc cctgctgtgc agcggcatgc
      661 tggacgggat cagcgtcgtc ataagaggca ccatcgtaca ggtgaagacc cccgatccca
      721 tgcggggaag ggtatcctcg gtcaattcca tgttcatcaa ttccagtaac gagctggggc
      781 aattcgagag cggggtgatg gcgcggctac tcggtgtcgt accctccgtg gtatttggtg
      841 gttgcatgac catcctcgtc gtgatcacca cctggttcag ggcgccaacc ctgcggaagc
      901 tggagtattg atcgcgggac tgcgtccggc gatacggatt ttcgcaggaa gattgcagca
      961 tgaacatggg ctcatggcag cgaagtgatg agccgctcga gcacaaaata aacaatgggg
     1021 caaaaggtgg agttcttctg cgtaagcgcg ggccggcgga ccaggacatc ttcgtccgta
     1081 taggggaagc gttcgcagtc ggaaggcctg acctcataaa tgccgcagta gttatcggcg
     1141 cccaggaagg ggcagggtgt cgttttcacc acgtaatccc catccgcgtc cagccgcagg
     1201 taacgcccga tgaaatcact ttcctttagt ttgaggtgtt tggcgatgcg cttgatatcc
     1261 ggcgttttga accggggtga gtaatgcttg cagcaattgg cgcattggag acagtcgacg
     1321 cgctcaaaag cctcctcgtg cagccggggc agttgctcca ggacccggtt cctgacggca
     1381 ggtttttcag ccttttccag gaaccgccgg tagtcctttt gcctgtcctt tgacttcttc
     1441 tcccaattat tcaataaatc actcatatat aagcgtttgc actcagaaac agaccacagg
     1501 cctgttggcc gaaggccccg acataagcca ttgggcgcca aagggatatt ttactcgtcc
     1561 atagccctta ggcacccttt aacaatacgc taccgcaaag gtgcgttatt tgccttaatt
     1621 ttaagttgtg gaaaacctta gggaacaaat actcattttg atcagtacgc cgatctatct
     1681 catcatcatc ggcctggaga tcctgctcag caattatcgc catcgcaaga cctacacgtt
     1741 taaagatacg ctttcgaacc tttatttaat gctgctcaac agcgggatcg acctgctttt
     1801 ccgcggcatt tatctggtgg tccttcagtt ctgctaccgg caccactgga tatcgatcac
     1861 caatgtcttt tcctactggc tgctgttgct gctggccgag gacttcctgt actactggct
     1921 gcaccgctgg gatcatgaga tccggttctt ttgggcagtg catgtgaccc accattcttc
     1981 ggagcacatg aattttacgg tgggcttccg gtcttcggta ttccagccgc tttaccggtg
     2041 ggtttatttc atcccgctgg cgctggtggg tttcaaaccc ctcgatatcg tcttcgtata
     2101 ttccgccacc cagatctggg gcatttttgt ccataccgag ctcatcggca agatgggctg
     2161 gctggaatat atcctggtca ccccctccca ccaccgggtg caccacgctt ccaatccgcg
     2221 ttacctggat aagaatatgg ggatgttcct gatcatctgg gaccagttgt tcggaacctt
     2281 ccaacccgag ctgacaccgg aagaatacca gcccacccga tacgggctga ccaagccggt
     2341 ggaaaagccc ggcccgtccc gtctcgtctt ccatgaatgg cgggccatcg gcaaggacct
     2401 cgcgcggcgg gatatcggct ggaaggaaaa atggcaatat gtttttggcc ctccgggatg
     2461 gagccatgac ggcagccgcc agaccagcga gcagatgcgg gaagcggaag ccgttgaaaa
     2521 tctggacttt gcaccggacc ctcaatttta atttcctttt aatatattct gttctccttt
     2581 tcggtgatgg ggaggtactt ttgtaccagc aattcattca tgcctacgca cgaatagttt
     2641 agcattcaat ttttagggtt taggggtttt taagaaaaat ggccagtccc aatttgtcaa
     2701 agtagggacg gccatttttc atttttgtct ttagcggcct ccggccgctg gggggccagc
     2761 tccagtttgt caaaatcgga gcggcccatt ttcatttggc ccattttcat ttaatgccct
     2821 ggaaagggcg ccaggcctcc ggccgccggt gggccagctc cggttttgca cctcttcccc
     2881 accacttcgt caatttgcaa tgaattttcc ctgcatcgtc gtacctttgc aaatccttca
     2941 aaaggtcaac cctcaaatat gaatctattc gaacagcaag cgaatgaatt tgccgggcgg
     3001 cacatcggtc cgaacgaaaa agaaacctcc ggaatgctga gaaccatcgg agcaggctcc
     3061 ctcgacgaac ttatcgacag aacgatcccg gcctcgatcc ggagcaggaa atccctgaat
     3121 atccccggtc cgctgagcga gcaccagtac ctcaccagcc tgcggcagat agccggtctc
     3181 aataaagtat tcaaatccta tatcggccag ggttattatg acacgatcgt gccgagcgtg
     3241 atcctgcgga acgtattcga gaacccgggc tggtataccc agta
//
LOCUS       DATGFG010000528         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_585864, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000528 DATGFG010000000
VERSION     DATGFG010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585864"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VKQ52_09895"
     CDS             complement(<1..1102)
                     /locus_tag="VKQ52_09895"
                     /inference="COORDINATES: protein motif:HMM:NF023504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87543.1"
                     /translation="MSLPKEPRQKMINMMYLVLTALLALNVSVEVLNAFKTVNHSIEK
                     SNKVIDEKNNLTYAAFTREVNDPQTMAKAKTWAPPAQQVQKLAADLSSYLDNLKEKLI
                     AASSPKVDKDGKREFADGNLDAATRLMDKEGEGPKLYERLKKYRLDLLAVIDPAKYPD
                     ESDKVKEDLKRTQDEFAHQLPLDTHIPPSESGNTAISNDSAKNWTVRYFHMTPTIAAL
                     TILSKFQSDVKNSESQVVDYLHKQVGEVKVIFDKFEPLVSPSATYLMPGDELEVKAGI
                     GAFSAAAKPKITINGSTLPLGEDGTADWKSRVSSTGTVNVKIEYVKPDGSIGTITKDV
                     KYTVGQPSGASVFLEKMNVLYVKEENPVKISGG"
     gene            complement(1193..1969)
                     /gene="gldL"
                     /locus_tag="VKQ52_09900"
     CDS             complement(1193..1969)
                     /gene="gldL"
                     /locus_tag="VKQ52_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HLZ87544.1"
                     /translation="MSAGVSKSTERLINIIVCVGAAVVIFGALQKILHTALADFFLTA
                     GLLTEALIFLVYAFLPPPGGEMHALVEALPKLASSGTSGNPALDKMDKMLQEADITPA
                     NLTKLSDGFKKFGTTVEKISDVSGALAATTDYTNKTKEAALALDSMKGAYQSATNTIT
                     SFNAAADSTKQFHSQVQVLTKNLGSLNAIYELELQDTNNHLKAMNSFYGNLVKASETM
                     QGSVADAKKTQEQIALLAKNLGNLNHVYGNMLSAMQGRQA"
     gene            complement(2114..3397)
                     /locus_tag="VKQ52_09905"
     CDS             complement(2114..3397)
                     /locus_tag="VKQ52_09905"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLZ87545.1"
                     /translation="MKNLSVLTAFFALFLMAGCKLGGKSGKGLQDDGQLHGVAPASAY
                     NLSKPPGMVYIPPGTFHMGPSDEDMNYSYTSRNRQTSISGFWMDATEITNNEYRQFTN
                     WVRDSIAATLMQYGKDVDGRFQIDWKKTATIKWGDKATIEKINAIIVTPENRIFGRKE
                     IDPSKLKFHSEVFDLKEAAAKGNEGKPRSQFINKHDVPVYPDTLVWIRDFSYAYNEPM
                     TKRYYSHPAFGNYPVVGVNWNQATVFCEWRTQYLNAHLEVKKMATESNFRLPTEAEWE
                     YAARGGRSQSMYPWGNYYLRNKRGCLLANFKPGRGNYPEDGGFYTVRADAYWPNDFGL
                     YCMAGNVAEWTSSFYYEGSYNFQHDMNPDIRWNAKDSDPPRMKRKVIRGGSWKDVGLY
                     LQTSARTYEYQDTAKSYIGFRCVIDLSPTITNNRR"
     gene            complement(3580..>4343)
                     /locus_tag="VKQ52_09910"
     CDS             complement(3580..>4343)
                     /locus_tag="VKQ52_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87546.1"
                     /translation="GEERYRDMATGNMAFLRTRLKGEGIHFYNHAYKGAARVPAFLDD
                     YALLIDALVQLQDITGDAGYLAEAGEIMRFVIGHFSEPETGFFFYTHDGQKDILLRKR
                     EIYDGATPSGNSMMASNLLYLGVVFDQREWIGRASRMAAALTRPVTGYPGSFGVWATI
                     FQALTYTIPEVVITGHRPENARKEFLAHLIPYRVFQSSPEENTQFPLLRDKPAGREPL
                     IFVCQGYACQLPVTEVATAIRLVENVYKFSRFDVQ"
BASE COUNT          933 a         1105 c         1199 g         1106 t
ORIGIN      
        1 tgccaccgga gatcttgacg gggttctctt ccttcacgta cagcacgttc attttctcga
       61 ggaagacgga ggcgccggaa ggctgaccga ccgtatactt cacgtccttg gtgatcgtgc
      121 cgatgctgcc gtcgggcttg acatattcga tctttacatt taccgtaccg gtagaggaga
      181 ccctggactt ccaatcggca gtgccgtcct cacccagcgg gagggtagaa ccgttgatcg
      241 tgatcttagg tttggcagcc gcgctgaaag cgccaatacc tgctttcact tccaactcgt
      301 cacccggcat gaggtaggtt gcactggggg aaacaagggg ctcgaatttg tcgaatatga
      361 cttttacttc accaacctgc ttgtggaggt aatccacaac ctgcgattcg gagttcttta
      421 catcgctctg gaatttgctg aggatggtaa gagccgcgat ggtcggggtc atatggaaat
      481 accgtacggt ccagttcttg gcgctgtcat tgctgatggc ggtgttcccg gactcggagg
      541 gcgggatatg cgtatccagc ggcagctggt gggcgaactc atcctgtgtc cgcttcagat
      601 cttccttcac tttatccgat tcatcggggt atttcgccgg gtcgatgacg gccaggaggt
      661 cgagccggta ttttttcagt ctttcgtaca gcttggggcc ttcgccttcc ttgtccatga
      721 gacgggtggc ggcgtcgagg ttaccgtcgg cgaactctct tttaccgtcc ttgtccactt
      781 tgggcgaaga ggcggcgatc agcttttcct tcaggttgtc aagatagctg gagagatcgg
      841 cggccagctt ctgtacttgt tgagcgggcg gcgcccaggt cttggccttg gccatggtct
      901 ggggatcgtt cacctcccgg gtaaatgcag cataggtcag gttgttcttt tcgtcgatga
      961 ccttgttgga tttttcgatg ctatgattca cggtcttgaa ggcattcaac acttccaccg
     1021 atacgttcag cgcgagcagc gccgtcagta ccaggtacat catgttgatc atcttctgcc
     1081 gcggttcttt aggtagagac atgggtcagt aattatttgt actggttaga atagtttttc
     1141 atattaggtt catgcgggcc ggaaaaaacg ggctcaggcg aaaaggctct ttctaagcct
     1201 ggcggccttg catggcgcta agcatgttcc catagacatg gttaaggtta cccaggttct
     1261 tggccagaag ggcaatctgc tcctgtgtct tcttggcatc ggcaacgctg ccctgcatgg
     1321 tctcggaagc tttcaccagg ttaccataga agctgttcat ggctttcaga tggttgttgg
     1381 tatcctgcag ttccagttca tagatcgcgt tcagcgaacc gaggttcttg gtcaggacct
     1441 gcacctgcga atggaattgc ttggtgctgt cggcagcggc gttaaagctc gtgatggtgt
     1501 tggtcgcact ctggtaagca cctttcatgc tatcgagggc cagggcggct tccttcgtct
     1561 tgttggtgta atccgtagta gcagccaggg cgccggagac gtcgctgatc ttctctacag
     1621 tagtgccgaa tttcttgaaa ccatcgctca gcttggtgag gttggccgga gtgatatcgg
     1681 cttcctgcag catcttgtcc atcttgtcca gggcagggtt accggaggtt ccactgctgg
     1741 ccagtttggg gagggcttcc accagggcgt gcatttcacc accgggaggc gggaggaacg
     1801 catacaccag gaagataagc gcctctgtca gcagaccggc ggtaaggaag aagtcagcca
     1861 gggcggtatg caggatcttt tgaagggcgc cgaaaattac gacggcagcg ccaacgcaca
     1921 caatgatatt gataagtctt tcggtagatt ttgatactcc agcgctcata gtgattaatt
     1981 tttaaattgt ttgtaaagga ttttaaaggg ttatggaaaa aagagatcct gttgattttt
     2041 agttagtctc tcaaatcaaa aacgtgtatt ctacggaaaa tattttggta cccggaagat
     2101 aatctgtagc cggctatcga cggttgttcg ttatcgtcgg cgacagatcg atcacacaac
     2161 ggaaaccgat gtatgattta gcggtgtcct ggtactcata cgttctggcg ctggtctgca
     2221 ggtataagcc tacgtctttc caactgccgc cacggatgac cttacgcttc attcgggggg
     2281 gatcggagtc cttggcgttc cagcggatgt cggggttcat atcgtgctgg aagttgtaag
     2341 atccttcata gtagaaggaa gaggtccact ccgccacatt accggccata cagtacagac
     2401 caaagtcgtt gggccagtaa gcatcggccc gtacggtata gaaaccgccg tcttcaggat
     2461 agttaccgcg tccgggtttg aagttggcga gcagacaacc tcttttattt ctaaggtagt
     2521 agttacccca gggatacatc gattgggaac gaccgccacg ggccgcatat tcccattccg
     2581 cttcggtcgg cagccggaag ttcgactcgg tagccatttt cttgacttcc aggtgagcat
     2641 tcaggtactg cgtccgccat tcgcagaaaa cggtggcctg gttccagttg accccaacga
     2701 cggggtaatt gccgaaagcc gggtgggaat agtaccgttt ggtcatgggt tcgttatacg
     2761 catagctgaa atcgcggatc cataccagcg tatcgggata caccgggaca tcgtgcttgt
     2821 tgatgaactg gctgcggggc ttgccttcat tgcccttcgc ggcagcttcc ttcaggtcga
     2881 acacctcgga atggaatttc agcttggaag gatcgatctc tttccggccg aagatgcggt
     2941 tctcgggagt aacgatgatg gcattgatct tttcgatcgt ggccttgtca ccccacttga
     3001 tggttgccgt cttcttccag tcgatctgga aacgaccatc gacatccttg ccatattgca
     3061 tgagggtcgc agcgatggag tccctcaccc agttggtgaa ctgccggtat tcgttgttgg
     3121 tgatctcggt agcatccatc cagaaaccgc tgatagacgt ctgcctgttg cgggaagtgt
     3181 aggaatagtt catatcctcg tcgctgggcc ccatgtgaaa ggttcccggc gggatataga
     3241 ccatgcctgg aggtttggaa agattgtacg ccgaggcggg agccacgcca tgcagctgcc
     3301 cgtcgtcttg taatcctttg ccggattttc cacctagttt acagcctgcc attaaaaaga
     3361 gggcaaaaaa ggccgttaaa acggataggt ttttcatttt cataatatta aagggtattg
     3421 ggcaaaaaaa gtcttttgac aacatctcgc tacaagttta aaattatttt ttcgaaagtc
     3481 aaagcttcgt accgatcaaa tccgtttata ctgaccggtt aaaaccccgc tttgaactgg
     3541 tttccagatg ctaaagtaat aaacgcgacc caggccggat tattgtacat caaaacggct
     3601 aaacttatac acattttcga cgagacggat tgctgtagcc acctcagtta cagggagttg
     3661 gcatgcgtac ccttggcaca cgaagatcag gggttcccgg cctgccggct tgtccctgag
     3721 caaagggaat tgggtatttt cctccggtga agactggaaa acccggtaag ggatcaggtg
     3781 agccagaaac tccttccggg cattttccgg ccggtggccg gtaataacta cctccggaat
     3841 agtatacgta agcgcctgaa agatggttgc ccaaacgcca aaagacccgg ggtagccggt
     3901 gaccggccgg gtcagggcgg cggccatccg gctggcccgc ccgatccatt cccgctggtc
     3961 gaaaacgaca cccaggtata acaaattgct tgccatcatg gaatttcccg agggcgtagc
     4021 cccgtcatag atctcccttt tgcgcagcag gatgtctttc tgcccgtcgt gagtatagaa
     4081 gaagaaaccg gtctcgggct cgctgaaatg cccgatcaca aaacgcatga tctcgcccgc
     4141 ttccgccagg taacccgcat cccccgtgat atcctgcaac tgcaccagcg catcgatcag
     4201 cagggcataa tcgtccagga aggccggcac ccgcgccgcg cccttatagg catggttgta
     4261 gaagtggatg ccctccccct tcagccgggt ccgcagaaac gccatattgc cggtcgccat
     4321 gtcccggtaa cgctcctcgc caa
//
LOCUS       DATGFG010000529         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_910614, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000529 DATGFG010000000
VERSION     DATGFG010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910614"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VKQ52_09915"
     CDS             <1..764
                     /locus_tag="VKQ52_09915"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HLZ87547.1"
                     /translation="EHYTMNLTLKVWRQKNKKDKGGFETYSVSGISSEMSFLEMFDVL
                     NERLVVEGKEPIAFDHDCREGICGMCSMYINGRPHGPWEGTTTCQLHMRAFKDGDTIV
                     VEPWRANAFPVVKDLVVDRSAFDRIIQAGGYISVNTGNAVDANAIPVEKDKADTAFAA
                     AACIGCGACVAACKNSSALLFVSAKVSHLALLPQGSPERKTRVADMVAQMDKEGFGSC
                     TSTGACEAVCPKEISITNIARLNAEYGRAGLTADK"
     gene            859..2511
                     /locus_tag="VKQ52_09920"
     CDS             859..2511
                     /locus_tag="VKQ52_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ87548.1"
                     /translation="MIRVNNVTLSFGKRVLFDEVNLTFTKGNCYGVIGANGAGKSTFL
                     KILAGDIEPSKGAVEIAPGQRMAVLNQNHFAFDEDTALNTVLKGHKSMWKVMQERDTI
                     YAKTDFTEQDGIRAGELEGEFGEMGGYTAESDAAALLSDLGVKESLHANLMKELPGAM
                     KVRVLLAQALFGNPDILILDEPTNDLDVETINWLENFLAEYENIVIVVSHDRHFLDAV
                     CTHVADVDRQKIKIFTGNYTFWYESSQLMARQLSDKNKKNEDKRAALLEFIARFSANA
                     SKSKQATSRKKALEKLTIEEIEPSNRRYPGIIFKQLREVGNQILNIEKLSKKAENGRF
                     LFSNVTFSVNKGDKIAFVSKDHLALSTFFRLINGEVTADGGKFEWGTTVTKAYLPNDN
                     SEYFEGEQINLMDWLRQFVPPHVTDVDEPFLRGFLGKMLFSGDEVLKNTHVLSGGEKV
                     RCMLSRMMLQDPNVVILDEPTNHLDLESIQAFNESMSNFKGIVLLTSHDHTFMQTVAN
                     RVIELTPGGVIDRLMSFDEYLEDARVKQLREEMYPREQLVKA"
     gene            2614..>3094
                     /locus_tag="VKQ52_09925"
     CDS             2614..>3094
                     /locus_tag="VKQ52_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87549.1"
                     /translation="MIRRLLILFLFFCGACLAARSQQPGGDTAKKAGIDTVGKGIDTA
                     QKPVDSAARADSLHRADSVRKAADSLHKSDSLKKVKADTLLSAAALLRQLDSLNTAPA
                     GPVDSLKMKLDAQLNGAANQRPGVSPGMPGGLQRQGANPGYAPSLGAGNGSGGASPSA
                     "
BASE COUNT          698 a          762 c          994 g          640 t
ORIGIN      
        1 tggaacatta tacgatgaac ctcaccctaa aggtctggag gcaaaagaac aagaaagaca
       61 aaggcggttt tgagacctat tccgtcagcg ggatctcttc ggagatgtcc tttttggaga
      121 tgttcgatgt gctgaatgaa cgactggtgg tggaaggcaa ggagcccatc gcgtttgacc
      181 atgactgccg ggaggggatc tgcggcatgt gttccatgta tatcaatggc cggccccatg
      241 ggccctggga gggaacgacc acctgccagc tgcacatgcg ggcttttaaa gatggcgata
      301 ccatcgtggt cgagccctgg cgggccaatg ctttcccggt ggtgaaggac ctggttgtcg
      361 accggagcgc atttgaccgg atcatccagg ccggcgggta tatctcggtg aatacgggga
      421 atgcggtgga tgccaatgcc atcccggtcg agaaggacaa ggcggatacg gcttttgcgg
      481 ccgcggcctg tatcggctgc ggagcctgcg tggcggcctg taagaacagt tcggcgctgc
      541 ttttcgtgtc ggcgaaggtg tcgcacctgg cgttgttgcc gcagggcagt cccgagcgga
      601 agacgcgggt ggccgatatg gtcgcgcaga tggacaagga agggtttggc agctgtacgt
      661 ccaccggggc ctgtgaggcg gtttgtccca aagagatctc catcaccaat atagcgaggc
      721 tgaatgccga gtatggccgg gccgggctga cggcggataa atagaaaccg ggctatcttt
      781 ttgcccggat aaaatgccgt ttcaacaccc ggacaaaatg ctacctttac agccagataa
      841 aatgttactt ttgcgcccat gatcagagtg aataatgtca ccctttcctt tgggaaacgg
      901 gttcttttcg atgaagtcaa tcttactttt acaaagggca attgctatgg ggtcatcggc
      961 gccaacggcg ccgggaagtc tactttcctg aaaatactgg cgggggatat cgagccttcg
     1021 aagggggccg tcgagatcgc ccccggacag cggatggccg tgctgaacca gaaccacttt
     1081 gcctttgacg aggataccgc gctgaatacg gtgctgaaag gacacaagag tatgtggaag
     1141 gtgatgcagg agagggacac catctatgca aagacggatt ttaccgaaca ggatggcatt
     1201 cgcgcgggag aattggaggg agagttcggc gagatggggg ggtataccgc ggagagtgat
     1261 gccgcggcgc tgctcagtga cctgggggtg aaagagtccc tgcatgccaa cctgatgaag
     1321 gagctgccgg gcgccatgaa ggtgcgggtg ttgctggcgc aggcgttgtt cggcaacccg
     1381 gatatcctga tcctggatga accgaccaat gacctcgacg tagagaccat caattggctg
     1441 gagaacttcc tggccgagta tgagaatatc gttatcgtcg tttcccacga ccgtcacttc
     1501 ctcgacgcgg tgtgtaccca tgtggcggat gtcgaccgcc agaagatcaa gatctttacg
     1561 ggcaactata cgttctggta cgagtcttcg cagttgatgg cgcggcagtt atcggataag
     1621 aacaagaaga atgaagataa gcgggccgcc ctgctggagt tcatcgcccg gttctccgcc
     1681 aacgccagca agtccaagca ggctacttcc cggaagaagg ccctggaaaa gctgacgatc
     1741 gaggagatcg agccgtcgaa ccgccgttat cccgggatca tcttcaagca gttgcgggaa
     1801 gtgggcaacc agatcctcaa tatcgagaaa ttatcgaaga aggccgaaaa cggccgcttt
     1861 ttgttctcca atgtgacctt tagcgtcaac aagggggata agatcgcttt tgtcagcaag
     1921 gatcatctcg cgctgagcac ctttttccgg cttatcaatg gggaggttac ggctgacggc
     1981 gggaagttcg aatgggggac gacggtcacc aaggcctatc tgcccaatga caacagcgaa
     2041 tatttcgagg gggagcagat caacctgatg gattggctgc ggcaattcgt cccgccgcat
     2101 gtcaccgatg tcgacgagcc gttcctgaga ggtttcctgg gtaaaatgct ctttagcgga
     2161 gatgaggttt tgaagaacac gcatgtgttg tccggtgggg agaaggtgcg ttgtatgctg
     2221 agccggatga tgctgcagga cccgaatgtg gtgatcctcg acgagccgac caaccatctc
     2281 gacctggaat ccatccaggc cttcaacgaa agcatgagca atttcaaggg gatcgtcctg
     2341 ctgacgtccc atgaccatac ctttatgcag acggtggcca accgggtgat cgagctgacg
     2401 ccgggcggag ttatcgaccg gctgatgtcc tttgatgaat acctcgaaga tgcgcgcgtc
     2461 aagcagctgc gggaagagat gtatcccagg gagcaactgg ttaaagcgta ggggtgggat
     2521 acggcgccgc ggtgtgccgg atacataggt ggttttgtcc tggtggatac aataaatatg
     2581 cagattcggc atttttaaga aaaaccacgg ttgttgatca gaaggctgct tatacttttc
     2641 ctcttctttt gtggcgcctg cctcgccgcg aggagtcagc aaccgggtgg cgatacggcg
     2701 aaaaaggccg ggatcgatac ggtcgggaag ggtatcgata ccgcgcagaa gccggttgat
     2761 tcggcggccc gggcggacag cctgcaccgg gcggattccg tcagaaaggc ggcggacagc
     2821 cttcataaga gtgattccct aaaaaaagtt aaggcggata ccctactttc ggcggcggcg
     2881 ctgctccgcc agttggactc attgaatacc gcgccggcgg ggccggtgga ttccctaaag
     2941 atgaagctgg atgcccagtt gaacggggcg gccaatcagc ggccgggggt gagcccgggg
     3001 atgccgggcg ggcttcagcg acagggggcc aatccgggtt atgcgccatc cctgggggcg
     3061 ggaaacggga gcgggggagc cagtccttcc gccg
//
LOCUS       DATGFG010000530         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_499554, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000530 DATGFG010000000
VERSION     DATGFG010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499554"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /gene="mraW"
                     /locus_tag="VKQ52_09930"
     CDS             complement(<1..375)
                     /gene="mraW"
                     /locus_tag="VKQ52_09930"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HLZ87550.1"
                     /translation="MTTAPNSEDNQGRHAYHLPVLYQESLEGLAIKPDGVYADLTFGG
                     GGHSRGILERLGPKGRLFAFDQDSDARRNLPDDSRITFLPHNFRHAGRFLRLHEVVPV
                     DGILADLGVSSHQFDEGQRGFSY"
     gene            complement(372..833)
                     /gene="mraZ"
                     /locus_tag="VKQ52_09935"
     CDS             complement(372..833)
                     /gene="mraZ"
                     /locus_tag="VKQ52_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HLZ87551.1"
                     /translation="MIGFLGEYEATLDTKGRFLLPVGFKKQLPEDGPTQFVINRGFEK
                     CLSLYPMKSWEPIFARVSQLNDFDPEVRQFRRYFLNGATMVELDSAGRLLIPQNLKEH
                     AGLEKDIVLAAAVDKIEIWDKSKYQQFFESFSPEAFSSLAKQVMNPGGLSS"
     gene            1087..1497
                     /locus_tag="VKQ52_09940"
     CDS             1087..1497
                     /locus_tag="VKQ52_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87552.1"
                     /translation="MPGAGYSGTPLLKKLGIKADWKVRLIGAPADYFTLLGTGLPGQL
                     CSAREVPDLIHLFAPTRKDFEKEMQAALRARDRNPQLVVWVSWYKKSSGKATDLTEDI
                     IRNYALANGLVDIKVCAVSEDWSGLKLVVPLSRR"
     gene            1584..>2202
                     /locus_tag="VKQ52_09945"
     CDS             1584..>2202
                     /locus_tag="VKQ52_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HLZ87553.1"
                     /translation="MQPQDISIADYRYTLPPEQIAFHPLPERDASRLLILREGVITDD
                     SYRHIAGHLPEESLLVFNNTKVVEARIEFRKETGARIEIFCLEPPADYGGIAAALAQT
                     GKVRWKCLIGGASKWKRGQVLSKKITAAKGAASTGAMGGDAAAGAAGGGAGEELVLEA
                     RYLEKSEDAFLIELSWTPAELSFAEVLHQAGFIPLPPYIHREAEAA"
BASE COUNT          477 a          574 c          665 g          486 t
ORIGIN      
        1 ataggagaag cctcgttgcc cttcgtcgaa ctggtggctg ctcaccccca gatcggccag
       61 tatcccgtcg acgggaacga cttcatgcaa acgcaggaag cggccggcgt gacggaagtt
      121 gtgcggcagg aaggtgatgc ggctatcgtc cggcaggtta cggcgggcgt cgctgtcctg
      181 gtcgaaggcg aatagtctgc ctttcggtcc cagtctttcg agtatcccgc gggaatggcc
      241 gccgccgcca aaggtgaggt ctgcatacac cccatcgggt ttgatggcca gaccctcgag
      301 cgattcctga tacaggacag gcaggtgata ggcatgccga ccctggttat cctcgctgtt
      361 tggcgccgtc gtcacgatga caaccctccc gggttcatca cctgttttgc caggctgctg
      421 aatgcttccg gtgaaaaaga ttcaaagaac tgttggtact tactcttgtc ccagatttcg
      481 atcttgtcta cagccgcggc cagtacgatg tctttttcga gaccggcgtg ctctttcagg
      541 ttttggggga tcaacaaccg cccggcggag tccagttcga ccatcgtcgc cccattcagg
      601 aaatatcttc tgaattgacg aacttcgggg tcaaaatcat tcaattggct taccctggcg
      661 aatatgggct cccagctttt catcggatag aggctaaggc atttttcaaa tccacgattg
      721 atgacaaact gggtgggacc gtcctccgga agctgctttt tgaagccaac cggaagcagg
      781 aagcgacctt ttgtgtccag agtagcttcg tattcgccca gaaaaccaat catttatgag
      841 gagttgaatc aatctttact gataactaac acaaaataac actttttccc actttattga
      901 gggaaaacgg aaaatttaat ttttccacaa tagtgcattg tggctctgtg tgttgtcatg
      961 gatgggttta ggtgccggcc ggccggtttt tggccttttt cggctgtgca tggtctgtga
     1021 atagcggtgg aaaagtggtg gaaaaccgcg gttttgatgg ggataaccaa gatcaatcaa
     1081 ttttatatgc ctggagccgg ttactcggga acaccgttat tgaaaaagct ggggataaag
     1141 gccgactgga aggttcggct catcggcgcc ccggccgatt acttcaccct gctcgggacc
     1201 gggcttccgg ggcagctctg ttcggcgcgg gaggtgccgg atcttatcca tttatttgcc
     1261 cccacacgaa aggatttcga aaaggaaatg caggcggcgc tgcgcgcccg cgacaggaac
     1321 ccgcaactgg tggtctgggt gtcctggtat aagaaaagca gcggcaaggc caccgacctc
     1381 acggaggata tcatccgcaa ctacgccctt gccaatgggt tggtggatat caaggtgtgc
     1441 gccgtcagcg aggactggag cgggctgaaa ctggtagtgc cgttgagccg gcggtgaagc
     1501 ccggggcgac ctacgccgtg agccggggcc ggaaagccgt atatttacac cctaaaaacc
     1561 ggcggcagtt agccgctacg tatatgcaac cacaggatat ctccatcgcg gattaccgct
     1621 atacgcttcc tcccgaacaa attgcctttc atcccctgcc ggagcgcgac gccagcaggc
     1681 tattgatcct cagggaaggg gttatcactg acgacagcta ccgccatatt gccggtcacc
     1741 tgccggaaga aagtttactc gtattcaata ataccaaagt ggtggaggcc cgcatcgaat
     1801 tccgtaagga gacgggtgcg cggatcgaga tcttttgcct ggagccgccg gctgactatg
     1861 gcggcattgc cgcggccctg gcgcagacgg gaaaggtgag atggaaatgc ctcatcggcg
     1921 gggcttcgaa gtggaagcgg gggcaggtgc tgagtaagaa gatcacggcg gcgaagggtg
     1981 cggcttcaac tggtgcgatg gggggcgatg cggcggcggg ggctgcgggc ggtggggcgg
     2041 gggaagaact tgtgctggaa gcccgttacc tggagaaatc ggaagatgcc ttcctgatcg
     2101 aactgtcctg gacaccggcg gagctgagct ttgccgaagt actgcaccag gctggattca
     2161 tccctctccc accctatatc caccgcgagg cggaagccgc gg
//
LOCUS       DATGFG010000531         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1655728, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000531 DATGFG010000000
VERSION     DATGFG010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655728"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /gene="smpB"
                     /locus_tag="VKQ52_09950"
     CDS             <1..378
                     /gene="smpB"
                     /locus_tag="VKQ52_09950"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HLZ87554.1"
                     /translation="MVLAGTEVKSIRDGKVNFVDSFCIFFQGELWVRNMHIAEYRFGT
                     TNNHIAVHDRKLLLTKRELRKLENKIKEKGYTIVPLRIFFNEKGYAKLEIGLGKGKKQ
                     YDKRETIKQRDTQKEIKRYLNRQ"
     gene            482..1549
                     /locus_tag="VKQ52_09955"
     CDS             482..1549
                     /locus_tag="VKQ52_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87555.1"
                     /translation="MQKFVYICALLAIHGVLSAQTVSGSWYGHADVEMAGIHNNYLTE
                     LVLKQKGTRVEGIFGYYFRDKYQSFFIHGRYNPQTREITILNIPVTFYASTTTVNSVD
                     CNTNFKGTLLLSRAGSTVRGALISDERYRYTCPEIKVSYALDKTDNETDSTISAGVAS
                     ARIWKPQADDLVVDAVTAAAPVQPQPALPAPVAEKPAPDTALAAAPPAGPATVTPPAD
                     PAKEESKKIEESFARRKPIVNRILEVESDSLRLSFYDNGEIDGDSISVFVNNQVVLTH
                     QGLAAKAFNLFLRLDSTRDINEISMYAENLGRIPPNTALMVLTDGKNRYEVFMSSSLS
                     ENATIQVKRKKPKSSLASTQQ"
     gene            complement(1592..>2069)
                     /locus_tag="VKQ52_09960"
     CDS             complement(1592..>2069)
                     /locus_tag="VKQ52_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87556.1"
                     /translation="YLGSFEGEERLLVIYTDGNYELTDMELTQRFDADKILEIRKFDP
                     EKIITAIYLDNEKLQYNVKRFKIETTTLHNKFLFIKEGEGNRLELVTLHPEPVVVIKS
                     GRGAQAKTQKIKIAGFVEVMGWKAVGSKLFDFSKSVEFEWGHKAEAKETPPQQELF"
BASE COUNT          505 a          707 c          458 g          399 t
ORIGIN      
        1 atggtattgg ccggaaccga ggtaaaatcc atccgggacg gcaaggtcaa cttcgtcgac
       61 agcttctgca tcttcttcca gggggaactc tgggtccgga acatgcacat cgccgaatac
      121 cgcttcggca ccaccaacaa ccatatcgcc gtacacgacc gcaaactcct cctcaccaaa
      181 cgcgaactgc gcaaactgga gaataaaata aaggagaaag gctataccat cgttcccctc
      241 cgcatcttct tcaacgaaaa aggctacgcc aaactcgaga tcgggctcgg aaaagggaaa
      301 aaacaatacg acaagcgcga aaccatcaag caacgcgata cccaaaagga gataaaacgc
      361 tatctcaacc ggcaataaat ccaacttccc ggtcgtttat ggagtttgcg ccaattctca
      421 ttattgtggc acgaaaattt ctatttctac cagtaaaagg gccctgccct gttaccaccc
      481 gatgcaaaaa tttgtctata tatgtgccct cctcgccatc cacggcgtac tgtccgccca
      541 aaccgtgtcc ggctcctggt atgggcacgc ggacgtcgaa atggccggca tccacaataa
      601 ctacctcacc gagctcgtct taaaacaaaa aggcacccgc gtcgaaggca tcttcggcta
      661 ctacttccgc gacaaatacc aaagcttctt catccacggc cggtacaacc cccaaaccag
      721 ggagatcacc atcctcaata tcccggtcac cttctacgcc tccaccacca ccgtcaacag
      781 cgtggactgc aacaccaatt ttaaagggac cctcctcctt tcccgcgccg gcagcaccgt
      841 caggggcgcc ctcatcagcg atgagagata ccgctacacc tgcccggaaa taaaagtgag
      901 ctatgcgctg gataagactg ataatgaaac agatagcact atttcggccg gtgtcgccag
      961 cgcccggatc tggaaacccc aggcggatga tctcgtagtc gacgccgtca ccgccgctgc
     1021 tcccgtccag ccccagcctg ccctgccggc cccggtggcc gaaaagcccg ccccggacac
     1081 ggcccttgcc gcggctccgc ccgccggccc cgcgacggtg acacccccgg ccgacccggc
     1141 aaaagaagag agcaaaaaga tcgaggaatc cttcgccagg cgtaaaccga tcgtaaaccg
     1201 catcctggaa gtcgaatccg actccctccg gctcagcttc tatgataatg gggagatcga
     1261 cggcgacagc atctccgtct ttgtgaacaa ccaggtcgtc ctgacccacc agggcctcgc
     1321 ggcaaaagcc ttcaacctct tccttcgcct cgacagcacc cgcgatatca acgagatcag
     1381 catgtacgcc gagaacctcg gccgcatccc acccaacacc gccctcatgg tcctcaccga
     1441 cggcaagaac cgctacgagg tctttatgag cagcagcctc tccgagaacg ccaccatcca
     1501 ggtaaaacgc aaaaagccaa aaagctccct ggcctccacc cagcagtaaa cgtcctcaac
     1561 cgcccgccgg caaccgggca agccgccctt cttagaacag ctcctgttgc ggcggggttt
     1621 ctttcgcttc cgctttatgc ccccactcga actccacact cttgctgaag tcgaataatt
     1681 tactgccgac agccttccag cccatcacct ccacaaaacc ggcgatcttg atcttctgcg
     1741 tcttggcctg cgccccacgc cccgatttga tcaccaccac cggctcggga tgcagcgtca
     1801 ccagctccag ccggttgccc tccccttcct tgatgaacag gaatttatta tgcaaggtgg
     1861 tcgtttctat cttgaaccgc ttcacgttgt actgcagctt ctcattgtcc aggtagatgg
     1921 ccgtgatgat cttctccggg tcgaatttcc ggatctccag gatcttgtcg gcatcgaacc
     1981 gctgcgtcaa ctccatatcg gtgagttcat agttcccatc ggtatagatc accagcagcc
     2041 gctcctcccc ctcgaaactc cccaggtat
//
LOCUS       DATGFG010000532         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1243688, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000532 DATGFG010000000
VERSION     DATGFG010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243688"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..920
                     /locus_tag="VKQ52_09965"
     CDS             360..920
                     /locus_tag="VKQ52_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87557.1"
                     /translation="MRKTLGVLSPFIFILILFACKKSGTGASQGLSGTWAFLGMHTQT
                     QTTSNVGGGVTLVANTTYNTKNNVGTITFSTDSMVISGLGYSVDTSYMAYFVFNNTVY
                     DSSAQTLSLTIPPTSASAKYQVVGSDSLYFPGGGILTALDSSATQGQGSHYVIHGDSL
                     TLTTRGVDTSGGALTVVNATIHLKRK"
     gene            1010..2341
                     /locus_tag="VKQ52_09970"
     CDS             1010..2341
                     /locus_tag="VKQ52_09970"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ87558.1"
                     /translation="MKNSRRTFLQQSSALVGGAVFLQAFGTHPFSFSGGVAPSDQINV
                     GAIGLNGMGWADLSAALKLPGVNVVALCDVDRNVLDKRMKDLADKKIDTSRIRTYGDY
                     RKLLAQKDIDVVIIGTPDHWHALIMIEACMAGKDVYVEKPVGNSIGECRAMMAAQKKY
                     NRVVQGGQWQRSQQHFKDAVDFVYSGQLGNIRTVKVWCYQGWMKPGPVVPDSAPPAGV
                     DYAMWLGPAPVRPFNPSRFHFNFRWFWDYAGGLMTDWGVHLLDYGLLGMKAQYPKTIA
                     ALGGNFAYPDLYEETPDTLTTLYEFDGFNLVWDHAMGIDNGSYGRDHGIAYIGNNGTL
                     VLSRGGWEVIEERQSKSKVSKPFEKVSDNGLDNHMANFIDVVRSRRMENLHCPIEAGS
                     FVATVAQMGNISLRSGQRLSWDKAAGKFTDDRVNQQFLMKEYHNGYKLPMI"
     gene            2525..3376
                     /locus_tag="VKQ52_09975"
     CDS             2525..3376
                     /locus_tag="VKQ52_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLZ87559.1"
                     /translation="MKRLATVAVCLTWGCRIAMAQDLKLDNRLAAGDTIKEVSITSGW
                     GSAPDTLPHRIDWQKAASYKQPFPFQSLFLPAAMIAYGATAVHNGALQKVNGEVKDQI
                     WEDNPHQPFHLDNYLMFAPTLSVYALNAAGVHGAHNFKDRTIILLMANVLANGTVFSV
                     KSWSHERRPDGSDYASFPSGHTAEAFVGAEFMRLEYKDVSPWYGIAGYAMATATGMLR
                     MYNDKHWLSDVVAGAGVGIASTRIAYWLYPKMQHLFGKGKNPDVVNVVMPTYNNGSFG
                     LSYVKTF"
     gene            complement(3458..>3846)
                     /locus_tag="VKQ52_09980"
     CDS             complement(3458..>3846)
                     /locus_tag="VKQ52_09980"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HLZ87560.1"
                     /translation="AADCEYNWQWYGDLVGGYFKSHPKQQEAKFIIVDKNNPATQGLP
                     DPWTHFDELYNYKYYAPDIKVLIKVDETSYTGGANGDNHPMAWYHDFEGGRSFYTALG
                     HTDATWSDPLFLHHLLAGIQYAMGKK"
BASE COUNT          834 a          867 c         1215 g          930 t
ORIGIN      
        1 cgtaaaggcg gccgggtttt ttcacggcgg tgtcgaggcg tggtttggga tgacggagga
       61 ggatgtggcg accgatgtcg ataatatcga caggggcgct tcctgagaat atatgcaggt
      121 gataggaggt atcccgggag gcagggtgtt ggtcgcggat ggtgtcctga ccatgaatgc
      181 agagggaaat gaggaaaaac ggaataaaga gataaagggg tttgggggcc ggcacctgtt
      241 ttggttaaaa atagaaagga taaatgtaag tgagatgtta atgacgcctg tattaccaat
      301 agagtactac tacacactaa atttggggtt ttttcgttat gggactcaaa gccaatagta
      361 tgcgaaaaac cctcggggtc ctgagccctt ttatctttat cctgatcctc ttcgcgtgta
      421 aaaaatcggg tacgggcgct tcgcagggtt tgagcggtac ctgggccttt cttggcatgc
      481 atacccagac gcaaaccaca tcgaatgtgg gcggtggagt aacgcttgtc gccaatacga
      541 cctataatac gaagaataat gtgggtacga taactttcag caccgattcg atggtcattt
      601 ccgggctggg gtactccgtg gatacttcct atatggccta ttttgtcttt aacaatacgg
      661 tgtatgactc gtctgcgcag acgcttagtc ttaccatacc gcctacttcg gcttcggcga
      721 aataccaggt ggtgggcagc gactccctct atttccccgg cggtgggatc ctgacggcgc
      781 tcgattcttc ggccacgcag ggtcagggaa gccattacgt gatccatggg gatagcctta
      841 cgcttaccac gcgtggggtc gataccagcg gcggggcgtt gacggtggtg aatgccacga
      901 tccatttgaa gcggaagtag ttgaacggcg gcgtggggcg ctgaaattgc aatcggttaa
      961 ataagttttt gtatattgaa ccggcgcagc taaatctgcg ttgcttcata tgaaaaattc
     1021 gcgacgtact tttcttcagc aaagctcggc cctggtgggc ggagccgtct ttctccaggc
     1081 ttttggcacc catccttttt ctttttccgg cggcgtggcg ccttccgacc agatcaatgt
     1141 gggcgctatc gggttgaacg gcatgggctg ggccgatctt tcggctgcgc tcaagttgcc
     1201 gggcgtcaac gtcgttgcgc tttgcgacgt cgacaggaat gtgctcgaca agcggatgaa
     1261 ggacctggcg gacaagaaga tcgatacgag caggatcagg acctatggcg actaccggaa
     1321 gctcctggcg caaaaggaca tcgatgtggt cattatcggt acgcccgatc actggcatgc
     1381 gctgatcatg atcgaggcct gtatggcggg taaggatgta tatgtggaga agccggtggg
     1441 caattccata ggggagtgcc gggcgatgat ggccgcgcag aagaaataca accgggtggt
     1501 acagggtggc cagtggcagc gaagccagca gcatttcaag gatgcggtgg attttgttta
     1561 ttcgggtcag cttggcaata tccggacggt gaaggtgtgg tgttaccagg ggtggatgaa
     1621 gcccgggccc gttgtcccgg atagcgcgcc tcccgcgggt gtcgactatg cgatgtggtt
     1681 gggccctgcg ccggtgagac cattcaaccc gagccggttc cattttaatt tccgttggtt
     1741 ctgggattat gccggggggt tgatgacgga ctggggggtt cacctgctgg actatggatt
     1801 gctggggatg aaggcgcaat atccgaagac gatcgccgcg ctgggcggca acttcgctta
     1861 cccggacctg tatgaagaga cgccggatac cctgacgacc ctgtatgagt ttgacggatt
     1921 caacctggta tgggaccatg cgatggggat cgacaatggc agctatggcc gggatcatgg
     1981 gatcgcctat atcggcaaca acggtacgct ggtgcttagc cggggaggat gggaggttat
     2041 cgaggagcgg cagagcaaga gcaaggtgag caagcctttt gaaaaagtgt ctgataatgg
     2101 attggacaat catatggcca actttatcga tgtcgtgcga agccgcagga tggagaacct
     2161 gcactgtcct atcgaggcgg gttcgtttgt ggccaccgtt gcgcagatgg ggaatatcag
     2221 tctccgcagc gggcagcggc tttcctggga taaagcggcg gggaagttta ccgacgaccg
     2281 ggtgaatcag caattcctga tgaaggaata tcacaatgga tacaagctgc ccatgatctg
     2341 agattgccca atggggctgc taaaacgatt ttgtgttaag cttcttaacc aaaatcggtg
     2401 ttaattcgcg gattgagtgt cgcaaacacc ccttacagcc tgattttatc aaaatttctc
     2461 ttcatttttg ccgttctaaa cgggataggg ccgtccataa ctaaacagta aagaaaagct
     2521 cattatgaag agactcgcaa ccgtagcagt ttgtctgaca tgggggtgcc ggatagcgat
     2581 ggcacaggat ctcaaattgg ataaccggct tgctgccggg gatacgatca aagaggtgag
     2641 tatcacttcg ggctgggggt cggctccgga tacgcttccg caccggatcg actggcagaa
     2701 agcggcttct tacaagcaac cttttccttt ccaatccctt tttcttcctg cggcgatgat
     2761 cgcctatggg gcaacggcgg tacataacgg cgcgttgcag aaggtgaacg gggaagtgaa
     2821 ggatcagatc tgggaagaca accctcatca accgttccac ctggacaatt acctgatgtt
     2881 tgcgcccacc ttatcggtat atgcgctgaa tgccgccggt gtccatggag cgcacaattt
     2941 caaggatcgg acgatcatcc tgctgatggc caatgtcctt gccaacggca ctgttttctc
     3001 ggtaaaaagc tggtcgcatg agcgcaggcc ggacgggtcg gattatgcgt cctttccatc
     3061 ggggcacacg gcggaggcgt ttgtgggtgc ggagttcatg cggctggaat ataaggatgt
     3121 ctctccctgg tatggtatcg ccggttatgc catggcgacg gccacgggga tgctccggat
     3181 gtacaatgac aagcattggc tgagtgatgt ggttgccggt gcgggtgtgg gtattgcctc
     3241 tacgcgtatt gcttattggc tgtacccgaa gatgcagcat ctttttggga aggggaagaa
     3301 ccccgatgtg gtgaacgtgg tgatgccgac gtataacaac gggtcgttcg ggctttcgta
     3361 tgtgaaaact ttctagcggc ggcgagcgca ggcgtgcggt ggggatggtt ttagtggcgg
     3421 ggggcagttt caggcatgcg gtgtgggatg tcggcagcta tttttttccc attgcgtatt
     3481 gaataccggc cagcaggtgg tggaggaaga ggggatcgga ccaggtggcg tcggtatgac
     3541 cgagggcggt gtagaaggat ctcccgcctt cgaagtcgtg gtaccaggcc atgggatggt
     3601 tatcgccgtt ggctccgccg gtgtaggagg tttcgtccac ctttatgagg accttgatgt
     3661 caggggcgta atacttgtaa ttatacagtt cgtcgaagtg ggtccagggg tcgggaaggc
     3721 cctgggttgc ggggttattc ttatcgacga tgatgaattt cgcttcctgt tgtttggggt
     3781 gtgatttgaa ataaccgccc accagatcgc cataccattg ccagttgtat tcgcagtccg
     3841 cggccg
//
LOCUS       DATGFG010000533         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_593701, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000533 DATGFG010000000
VERSION     DATGFG010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593701"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..764)
                     /locus_tag="VKQ52_09985"
     CDS             complement(30..764)
                     /locus_tag="VKQ52_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HLZ87561.1"
                     /translation="MKIGVVQTRPVKGDVAANLARHQRVASFAIERGVDLLVFPELSL
                     TGYEPELAKELATTKEDERFAGLQRMSDKYGIFIGAGMPIATEEGVQIGMIIFRPDDP
                     RLVYAKQYLHADELPFFVPGKEQVFLSLDDNKIAFSICYELSVPEHSVNAYSHRANIY
                     LSSVAKTVAGVEKALETLSGIACTYSMTALMANAVGPCSDGVCGGGSVVLDDQGEVLA
                     GLDEEGEGMLVYDTGTRKVDLCEFEE"
     gene            complement(770..1333)
                     /locus_tag="VKQ52_09990"
     CDS             complement(770..1333)
                     /locus_tag="VKQ52_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLZ87562.1"
                     /translation="MTELEQYLRSYMGVADEDMEVLVSAFYPATLEKGDFFLRAGRIC
                     DKLSFHREGLMRVFARHGEKEVTQWISFKGNFITDLQGIVCDEPSRFTVQALTRCELY
                     TIDKKDYLDLPRRIAKWATLERMLITRCFGFMETRVFSLLSMSGEERYAYLQGQNPEL
                     FDTVPLKYLASMMGMTPESLSRIRKKK"
     gene            1425..2111
                     /locus_tag="VKQ52_09995"
     CDS             1425..2111
                     /locus_tag="VKQ52_09995"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLZ87563.1"
                     /translation="MVLGHFGVGFGAKKFAPKISLGTLFIAVQWADLLWPVLLLLDVE
                     KVTIRHDNPNFPLDFVSYPFTHSLLMGIVWGLCFGLLYWLAKKDKRGAIVLAICVVSH
                     WVLDLVVHAPDLPLLPGDSPKLGLGLWNNQLATAIVEGLLFLVGLVLYLKTTRAKNTT
                     GKWTLWLLVILLVANQMAGRLSPLPPSGHAIGWAAQYQWIFVLLGYWVDRNRVTAHPL
                     PSPPPSYTAA"
     gene            complement(2062..2529)
                     /locus_tag="VKQ52_10000"
     CDS             complement(2062..2529)
                     /locus_tag="VKQ52_10000"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HLZ87564.1"
                     /translation="MEYQLAQVNVGRILGPMESPVMVEFAANLDRINAIAEGSPGFVW
                     RLKDGSNNATSIPVTEDRFLLVNLSVWRSIDDLFAYVYRSDHKDILRRRAEWFERMKE
                     MFLACWYVPAGHEPTVAEAMERVAYIRRNGPTPYAFTFKQRYTMEEAREAGVL"
     gene            2651..>3041
                     /locus_tag="VKQ52_10005"
     CDS             2651..>3041
                     /locus_tag="VKQ52_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87565.1"
                     /translation="MADRKHTFGEKLREILIEIFIIVFAVTLSIYLHSWSEHRHEQKV
                     VHEFLRGLKTDLTDDIPRIRAARDTIAKVQDNFEHAYSLHKGQLPDSIVGHYFIYREV
                     TTHLNSARYEGFKSSGKLDNIENESLRE"
BASE COUNT          710 a         1037 c          651 g          643 t
ORIGIN      
        1 gctaacccgg tggtcccttg tgccggtatt cactcctcaa attcgcatag atcgactttc
       61 cgggtcccgg tatcatacac cagcatcccc tcaccctcct catccaaacc ggccagcacc
      121 tcaccctggt catccagcac cacggacccg ccgccacaga ccccatccga acagggtccg
      181 acggcattcg ccatcaacgc cgtcatcgaa taggtacaag cgatcccgct cagggtctcc
      241 aacgccttct ccacccccgc aaccgtcttg gccacactgg aaaggtagat attcgcccgg
      301 tggctatagg cattcaccga atgctcggga acagacagct cataacaaat cgaaaaggct
      361 atcttattgt catccagtga tagaaacacc tgctccttcc ccggcacaaa gaagggaagc
      421 tcatccgcat gcaggtactg cttcgcatac accagccggg gatcatccgg ccggaaaatg
      481 atcatcccga tctgtacccc ctcctccgtc gctatcggca tccccgctcc gataaagatc
      541 ccatacttgt cactcatccg ctgcaacccc gcaaaccgct catcctcctt cgtcgtcgcc
      601 aattccttcg ccagctccgg ctcataaccc gtaagggaaa gctccgggaa aacaagcagg
      661 tccacccccc gctcgatagc gaagctcgcc acccgctggt gccgggcaag attcgctgcc
      721 acatcaccct ttaccggtct ggtctgtaca acccctattt tcatatcgtt tactttttct
      781 ttcttatccg gctgagcgac tccggggtca tccccatcat cgaagccagg tacttcaacg
      841 gtacggtgtc gaacagctcg ggattctgtc cctgaaggta ggcatatctt tcctcacccg
      901 acatcgacaa aagcgaaaac acccgcgtct ccataaaacc aaaacaacgc gtgatcagca
      961 tccgctcgag cgtcgcccat ttggcgatcc gccgcggcag atcgagataa tccttcttgt
     1021 cgatggtata cagctcacaa cgcgtcagcg cctgcaccgt gaaccgcgat ggttcgtcac
     1081 agacgatccc ctgcagatcg gtgataaaat tccccttgaa agagatccat tgcgtcacct
     1141 ccttctctcc gtgccgggcg aacacccgca tcagcccctc ccggtgaaaa ctaagcttat
     1201 cgcagatgcg tcccgctctg aggaagaaat cccccttctc cagcgtcgcg ggataaaagg
     1261 ccgaaaccag gacttccata tcctcatcgg ccacacccat gtacgaacga aggtattgtt
     1321 cgagctcggt cataccgcta atttcttacc atttgtcaag aaaacctcct gttcgcccga
     1381 ctaattttgc cggcgtagta tattacatcc aattttacac aactatggtc cttggacact
     1441 tcggcgtcgg cttcggcgca aaaaaattcg ccccgaaaat ctctctcgga accctattca
     1501 tcgcagtaca atgggccgac cttctctggc cggtgctcct cctgctcgat gtagagaaag
     1561 taactatccg ccacgataac cccaacttcc cgctggattt cgtgtcctac cccttcaccc
     1621 atagcctgct catgggcatc gtctgggggc tgtgcttcgg gcttctctat tggctcgcca
     1681 aaaaagacaa aagaggcgcc atcgtcctcg ccatctgcgt ggtaagccac tgggtcctcg
     1741 acctcgtcgt ccacgcaccc gaccttcccc tcctgcccgg cgactccccc aaactgggcc
     1801 tcggcctgtg gaacaaccaa ttggccaccg ccatcgtcga aggcctcctc ttcctcgtcg
     1861 gcctcgtgct ctatctcaaa accacccgcg caaaaaatac cacgggcaaa tggacccttt
     1921 ggctcctggt catcctgctg gtggccaacc agatggcggg ccggctctcc ccgctgcccc
     1981 cctccggtca cgccatcggc tgggccgccc aataccagtg gatcttcgtc ctcctcggct
     2041 actgggtcga ccgcaaccgt gtcacagcac acccgcttcc ctcgcctcct ccatcgtata
     2101 ccgctgctta aaggtaaacg cgtagggtgt gggcccattg cgccggatat acgctacccg
     2161 ctccatcgcc tcagccaccg tcggctcatg ccccgccggc acataccaac aggccaggaa
     2221 catttccttc atccgctcga accattccgc acgccgccgc aggatatcct tgtgatccga
     2281 ccggtataca taggcgaata agtcgtcgat cgaccgccat accgacagat tcacgagcaa
     2341 aaagcgatcc tccgtcacgg ggatcgacgt cgcattgttc gacccatcct tcagtctcca
     2401 gacaaaaccg ggactgccct cggcaatcgc attgatccgg tccaggttgg ccgcaaactc
     2461 caccatcacc ggactttcca tcggcccgag gatccggccg acattcacct gcgctaattg
     2521 gtattccatc tattttgtac tttagttcaa atctaagcca atgctcatgc ccgtacaagc
     2581 atctgaataa ttcatatatt taaccatgca ggacgaactc accaaacacg gcctaaaaat
     2641 atacaagacc atggcagatc gtaagcacac cttcggcgaa aaacttcggg agatcctcat
     2701 cgagatcttc atcatcgtct tcgccgtcac cctatccatc tatctgcaca gctggagcga
     2761 acaccgtcac gaacaaaagg tggtgcatga attcctccgc ggcctgaaaa ccgatctcac
     2821 cgacgacatt cctcggatca gggcggcccg tgataccatc gcaaaagtgc aagacaactt
     2881 tgaacatgcc tacagcttac ataaaggaca actaccggac tccatagttg gtcactattt
     2941 catctatcgc gaagtgacca cgcatctcaa tagcgcaaga tacgaaggct tcaaatccag
     3001 cgggaagctg gacaacatcg aaaacgagag ccttcgtgag a
//
LOCUS       DATGFG010000534         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1491005, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000534 DATGFG010000000
VERSION     DATGFG010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491005"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="VKQ52_10010"
     CDS             complement(<1..1746)
                     /locus_tag="VKQ52_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HLZ87566.1"
                     /translation="MKKYFTFLICLLLVLPGILMAGNILDKAGNPSATPVAAFSLRLL
                     SSSYAGKAIQVRRSSDNTVQDIGFTTAGDLDTAALKTFVGSGNGYISIWYDQSGNGIN
                     ATQATTSNQPSIVTAGAINRDNGKPAIYTSANGYLSYSGMSQFSGLYTATRMAVARSR
                     NGNSLAIIDGLGSYQLDCQMQSGTAIGIQVNTGSIVVNGAVANTNALAAINSIRNNGA
                     SKLYINGVLNASNTSSIGAFGSSVTGMIGVRNDLGAPGPGAFSETILFSSVLSDADRQ
                     AIDNSQISYYSLTNPLDKAGSPSARPVTAYSLRQLGSAYTGKASQVRRSSDNAIQDIG
                     FTAAGDLDTASLKTFVGSGNGYVSIWYDQGGKGINAIQATMANQPQIVSNGAVYRDNG
                     RPVMWTSTTGYLTYGPMSQFGGSYQATRMAVARSRNGNSLSIIDGLGPYQLDCQMQSS
                     TALGIQVNTGSIVVNGSTANTNVLTAVNAIRDNGASKLYVNGTLAGTNTSSIGTFSSS
                     LTGYIGVRNDLAGPSPGTFSETILFNSVLSDADRQAIDSSQNSYYTLVSPTNPLDRLG
                     SPSARPVTAFSLRKLG"
     gene            complement(1803..>2539)
                     /locus_tag="VKQ52_10015"
     CDS             complement(1803..>2539)
                     /locus_tag="VKQ52_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87567.1"
                     /translation="ICKIQATLGYGYAGLIPDMYLQDSIAAYYASVSLINGIGYIDWD
                     GEEFLFDQGLGNYSVKRFHRVLFQRTAAGGMPYLRIMGATLSEGAWHYQSVWNVGGGG
                     NMYDLPSRSWGIEGKDIRNVAYSNYFPATFGINAGIGSSSTVQPYENLEAISVGVGVT
                     YMLSLQQSSVESCPRKYGIFSAIRTWENARAAGAFPRMLKKQLADPTRYFHLEQVNNN
                     TWNLYTVDSSGNNKVLYQTLTRAPGY"
BASE COUNT          543 a          729 c          685 g          582 t
ORIGIN      
        1 tcccagtttt cgcaggctga aagccgtcac cggccgggcc gacggtgaac ccagcctgtc
       61 cagtggattg gtcggcgaca ccagtgtata gtaactattc tggctgctgt cgatcgcctg
      121 ccggtccgca tcggacagca cggaattgaa caggatcgtc tccgagaaag ttccgggact
      181 cggtcccgca agatcgttcc tcacgccgat atagcccgtt aacgatgagc tgaaagttcc
      241 tatgctgctg gtattggtcc ccgccagggt tccattcaca tacagcttcg atgctccatt
      301 atccctgatg gcattgaccg cagtcagcac attcgtattg gcagtgctcc cgttgaccac
      361 gatgctcccc gtattcacct ggatgcccag ggcagtggag gactgcatct ggcaatccag
      421 ttggtagggc cccaaaccgt cgatgatgga cagcgaattg ccgttccgcg atctggcaac
      481 cgccatgcgg gtggcctggt agctcccgcc gaactggctc atgggaccgt atgtcaggta
      541 cccggtggta gacgtccaca tcacaggccg gccattgtcg cgatagacag ccccattgct
      601 gacgatctgc ggctggttgg ccatagtggc ctggattgca ttgatccctt tacctccctg
      661 gtcataccag atacttacat agccattgcc gctgcccaca aaggttttca acgaagccgt
      721 atccaggtcg cccgctgcag taaagccgat atcctggata gcattgtcac tgctacgccg
      781 tacctggcta gctttacccg tgtaagccga gcccagttgc cgcaggctat aggccgtgac
      841 aggcctggcg ctgggggagc cggccttgtc caaaggattg gtcagggaat aatagctgat
      901 ctggctgtta tcgattgcct gcctgtcggc atcggacagc acggagctga acaaaatagt
      961 ctccgagaac gctccgggcc caggcgctcc caggtcattg cggaccccga tcatccccgt
     1021 tacagaagac ccgaaagctc cgatgctgct ggtattggag gcattcaaca ctccattgat
     1081 atataatttg gaagctccgt tgttcctgat ggaattgatc gccgccagcg cgttggtatt
     1141 ggctactgcc ccgttgacga cgatgctccc cgtattcacc tggatgccga ttgcagtccc
     1201 cgattgcatc tggcaatcca gctggtagct ccctaacccg tcgatgatgg caagcgaatt
     1261 gccattacgt gaacgggcga ccgccatgcg ggtggccgtg tagagcccgc tgaactggct
     1321 cataccgcta taagacaggt atccgttggc tgaagtataa atggcaggct tgccgttatc
     1381 ccggttgatc gccccggccg tcacaatgga cggctggttg gacgtagtag cttgcgtagc
     1441 gttgatgccg tttccgctct gatcatacca gatacttata tatccattgc cgctgcccac
     1501 gaaggtcttc agcgcggccg tatccaaatc ccccgcagtg gtaaagccga tatcctgcac
     1561 cgtattgtcg ctgctgcgcc gcacctggat cgccttgccg gcatatgaag agctcagcag
     1621 ccgcaggctg aaggcagcca caggagtcgc cgacggatta cctgctttgt ctaaaatatt
     1681 tcctgccata agaatgccgg gcagcaccag caaaagacag atcaggaacg taaaatattt
     1741 tttcatgatg acatctgtta tggttgatga aacatgtgaa tggaatgggg aggccaggcg
     1801 ggtcagtatc ccggcgccct ggtaagggtc tggtagagca ccttattgtt gccggaagaa
     1861 tccaccgtat acagattcca ggtattattg tttacctgct ccaggtggaa atagcgtgtg
     1921 ggatcagcca gctgcttctt cagcatcctg gggaaggcgc ccgccgccct tgcattttcc
     1981 caggtcctga tggcggagaa gatgccatat ttccgagggc atgattctac gctgctttgc
     2041 tgcaatgaca acatataggt aacccccacc ccaacggaaa tggcttccag gttctcgtat
     2101 ggctgaacgg tagaggagga cccgatcccc gcattgatcc cgaaagtagc ggggaaataa
     2161 tttgaatagg ccacgttccg gatatccttc ccttctattc cccagctgcg acttgggagg
     2221 tcatacatat ttccccctcc gccgacattc catacgcttt gataatgcca tgccccttcg
     2281 ctgagggtag cccccatgat gcgcagatag ggcatgcctc cagccgccgt tcgttggaaa
     2341 aggacccggt ggaagcgttt gacagaataa ttgccaagcc cctggtcaaa caggaattcc
     2401 tccccgtccc agtctatata gccgatcccg ttgataaggc tcacactggc ataataggcc
     2461 gctatggagt cctgcaggta catgtcggga atcagtcccg catacccgta gccgagcgtt
     2521 gcctggatct tgcagatcg
//
LOCUS       DATGFG010000535         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_154227, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000535 DATGFG010000000
VERSION     DATGFG010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154227"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VKQ52_10020"
     CDS             complement(<1..239)
                     /locus_tag="VKQ52_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87568.1"
                     /translation="MKKLLLATTLLGGLLLSAFRTAPGTLSPDERKYAIEYFQKTKAR
                     LLKDLKGLSEAQLSWKADSSRWSVYQCTEHIALAE"
     gene            354..1340
                     /locus_tag="VKQ52_10025"
     CDS             354..1340
                     /locus_tag="VKQ52_10025"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HLZ87569.1"
                     /translation="MRQIAFREALREAMNEEMRRDDRVFLMGEEVAEYNGAYKVSQGM
                     LDEFGPKRVIDTPISELGFAAVGVGAAQNGLRPIVEFMTWNFAVLALDQILNTASKML
                     AMSGGQVGCPIVFRGPNGSAGQLGAQHSTAFESYYANIPGLKVVSPSNGYDAKGLLKQ
                     SIRYEEDPVIFMESEVMYGDKAEVPAEEYYIELGKADIKKAGKDVTIVSYNKMMKVAL
                     GAAAELEKEGVSAEVIDLRTIRPLDWMTILESVKKTNRLVIVEEQWPFASISSEITYR
                     IQKEGFDYLDAPIRRITSADAPMHYAPNLVALALPDVGRTVKLVKEVLYMKK"
     gene            complement(1399..1536)
                     /locus_tag="VKQ52_10030"
     CDS             complement(1399..1536)
                     /locus_tag="VKQ52_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87570.1"
                     /translation="MKQNLELQAPPTGKILFKLTVLGCVLLGCVYGFLMLFNTALAAL
                     K"
     gene            complement(1689..2576)
                     /locus_tag="VKQ52_10035"
     CDS             complement(1689..2576)
                     /locus_tag="VKQ52_10035"
                     /inference="COORDINATES: protein motif:HMM:NF027464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HLZ87571.1"
                     /translation="MESRAPRFSGVMKLLLNTALTCLLCSCQQPAGSLHNPSPSLAPP
                     AANPSSPLASPSAISLAPHGENPYPTIGAIPLPPGYHRLPADHDPFTAWLRTVNLKKN
                     RTVYLFDGTPKRNQSAQFAVLDISVGHADLQQCADAVMRLRAEYYYATHDLDNIDFHT
                     EQGVRLNFRQWADHYGNTRASFNNYLNKVFTWCGTHTLEKQLVTRPLGSIRAGDVLIK
                     GGFPGHAMIVMDVAEDAKGNRIYLLAQSYMPAQDIHVVVNPTDPGLSPWYAADTTQGL
                     IETPEWTFTTNQLRTWPAE"
     gene            complement(2589..>2777)
                     /locus_tag="VKQ52_10040"
     CDS             complement(2589..>2777)
                     /locus_tag="VKQ52_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87572.1"
                     /translation="GSTWTFQALSLLPGYACHITTETGAGLTAAPETGITTETDTGAP
                     APAPVFHEISPADITARF"
BASE COUNT          613 a          568 c          970 g          626 t
ORIGIN      
        1 ttctcggcaa gggcgatgtg ttcggtgcat tggtataccg accagcggga actgtcggct
       61 ttccagctga gctgggcttc gctaagccct ttaagatctt ttagcaggcg ggcttttgtt
      121 ttttggaagt actcgatggc atatttgcgt tcatcggggg agagtgtgcc gggagccgtg
      181 cggaaagcag agaggaggag gccgccaaga agggtggtgg ccaggagtag ctttttcatg
      241 ttttggtgat tgtcccacga atatatatag atttgtcatc aagtaagtgg gtattccgaa
      301 tgatttgata atctccatga caaccaataa atttgcggct ctaaattgta ttaatgagac
      361 aaattgcttt tcgggaggct ctgcgcgaag ctatgaacga agaaatgcgg cgcgatgacc
      421 gggtattctt aatgggggaa gaggtggcgg aatataatgg cgcctataag gtaagccagg
      481 ggatgctgga tgagttcggc cccaagcggg tgatcgatac ccccatttcg gagttgggat
      541 tcgccgcggt gggtgtcgga gccgcacaaa acggccttcg ccctatagtg gagttcatga
      601 cctggaattt tgcggtacta gcccttgatc agatcctgaa tacggcgtcc aagatgctgg
      661 cgatgagcgg cggccaggta ggctgtccca tcgttttccg cgggcccaat gggtcggcgg
      721 gtcagcttgg tgcgcagcac tccacggctt ttgaaagcta ttatgccaat atccccggtc
      781 tcaaggtggt gagcccgagc aatgggtatg atgccaaggg gttgttgaaa cagtccatcc
      841 ggtatgaaga ggacccggtt atctttatgg agagtgaggt gatgtatggc gacaaggcgg
      901 aggtgccggc cgaggagtat tatatcgaac tggggaaggc ggatatcaag aaggcgggga
      961 aggacgtgac gatcgtttcc tataataaga tgatgaaggt ggcgcttggc gccgcggctg
     1021 aactggagaa ggaaggggtc agcgccgagg tgatcgatct gcggacgatc cgcccgctgg
     1081 actggatgac cattctcgag agcgttaaga agacgaaccg cctggtgatt gtggaggagc
     1141 agtggccctt tgcgtcgatc tcttcagaga tcacctaccg gatccagaag gaagggttcg
     1201 attacctgga tgcgccgatc cgccggatca cgtctgccga tgcccccatg cactatgcac
     1261 ccaacctggt agcactggcg ttgccggatg tgggaaggac ggtgaagctg gtgaaagagg
     1321 tgctgtatat gaagaagtaa aagaaaagaa aggcttataa taaaaaagcc cgccggttgg
     1381 cgggcttttt tattggagtt atttgagggc ggcgagggcc gtattgaaca gcatgagaaa
     1441 gccgtataca caacctaaca atacacaacc caagactgtc aatttgaaaa ggatcttacc
     1501 ggtaggggga gcctggagct caagattctg cttcataggg aaatactttt ttggattata
     1561 aaaatgttgg tttttaaata gtcattggat ggagctaaga tacggcaaag ggagatggaa
     1621 tgcaaggggt ttttacaagg aaatgtatga tttgtcgtat ttcgcaggta attgctaagg
     1681 gggactgctt attctgccgg ccaagttcgt aactgattgg ttgtgaatgt ccattccggt
     1741 gtctcgataa gaccctgggt ggtgtcggcg gcataccagg ggctgaggcc ggggtcggtg
     1801 gggttgacga cgacatggat atcctgggcc ggcatatagc tctgggccag gagatagatg
     1861 cggttgccct ttgcatcttc ggcgacgtcc atgacgatca tggcatgtcc cgggaagccc
     1921 cctttgatca ggacgtcacc ggcccggatg ctcccgaggg ggcgggtgac gagctgtttt
     1981 tccagggtat gggtgccgca ccaggtgaag accttattca agtaattatt gaagctagca
     2041 cgtgtattac cataatggtc agcccattga cggaagttta aacggacgcc ctgttcggtg
     2101 tggaagtcga tattgtccag atcgtgggtg gcgtagtaat actcggcgcg gaggcgcatg
     2161 acggcgtcgg cgcattgctg gagatcggca tggccgacgg agatgtcgag gacggcgaat
     2221 tgggcggact ggttgcgttt tggggtgccg tcgaagaggt agacggtccg gttctttttc
     2281 aggttcacgg tcctgagcca ggcggtgaag gggtcgtggt cggcgggcag gcgatgatag
     2341 ccagggggga gggggatggc gccgatggtg gggtatgggt tttcgccgtg gggcgcgagg
     2401 gagatggccg atgggctggc gaggggcgat gaagggttgg cagcaggtgg ggcgagagac
     2461 ggtgaagggt tatggaggga gccggccggc tgctggcagg agcagagcag gcaggtgagg
     2521 gcggtgttta gcaggagttt cataaccccg gagaagcggg gtgcgcggga ttccatacaa
     2581 aggatctgtt aaaaccgggc ggtgatgtcg gcgggggaga tttcgtggaa gacaggggcg
     2641 ggggcgggcg cgccggtatc ggtttcggtg gtgatcccgg tttccggggc ggcagtgagg
     2701 cccgctcctg tttcggtggt aatatggcag gcatacccgg gaagaaggga aagggcctgg
     2761 aaggtccagg tagagcc
//
LOCUS       DATGFG010000536         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_328435, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000536 DATGFG010000000
VERSION     DATGFG010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328435"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VKQ52_10045"
     CDS             <1..334
                     /locus_tag="VKQ52_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87573.1"
                     /translation="AAQKVIVDKNTGVIDLQKTLDGTGLLGGAFGLLPVNGQTLLTTI
                     TYKINYGSNNALQQIQVQLQYYDKKSLISAGLLNGLINKLDNVLSGNVITTSSAPRPP
                     LVIMTRYN"
     gene            637..>2505
                     /locus_tag="VKQ52_10050"
     CDS             637..>2505
                     /locus_tag="VKQ52_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ87574.1"
                     /translation="MALYRRADSLYHLDRSTRVTDSLALAGFTVVADELEKAGGRAGK
                     DTMLTGALLKKGILLDAGGDFARARVAYCRVLGYHPADDSLVFVTQVLVGTVYYNMDR
                     FDSASYFLLRAEALAGRFSDRDNRVRLYNTLGALYCDNGNFRQGKNYFDNALELVRGG
                     KLYDTAAAVSLQINIATASFRVGQYADALAIYRQLLGYRPLRDYVYENMGRAYVGMEN
                     YPAAMDWFRRVKVEQIPAVLNEMANAELRLHRLDSCVWYLRRLQELARMDAGARVRPV
                     DMGVNAYYTAEERSERGDLRGGLEALQRAIVVFAGNFNKRDPYGNPVSFSGAFAYYRL
                     FDALVRKAELYERSYEAGGRKNENDLVASYATYSAALSLLRYIEKSYATDEAKLFLKK
                     KSGPVHAEALSVCLRLDRLHPGGDYLEQAFLISERSKASVITANLEEQAFMGAPGAEQ
                     ELLQKVNNCKYRIARLNVKSEGALDSAGLAAITREKEGEETELSRLQKALEQDGEYYR
                     VRYGDASPGVRNLQRQLGRNEALVSLYAAGNMLHVFVVTKDGLRHVGMDSLAGMERDV
                     EGWLDKLKTTGGGVRFNGGETGRRLYSRLVQPIQVAAGGRTEWVIIPDGVFALLPFE"
BASE COUNT          491 a          514 c          953 g          547 t
ORIGIN      
        1 cgcggcgcag aaggtgatcg tggacaagaa taccggggtg atcgatctgc agaagacgct
       61 ggacggtacg gggctgcttg gcggggcgtt cgggttgttg ccggtgaatg ggcagacgct
      121 gctcacgacg atcacgtaca agatcaatta tggtagtaat aatgcgttgc agcagataca
      181 ggtgcagttg cagtactacg acaagaagtc gttaatcagt gcgggcctgc tcaacgggct
      241 gatcaataaa ctggataatg tgctgagcgg aaatgtgatc acgacctctt cggctccgcg
      301 gccgccgctg gtgatcatga cgagatataa ctagctttta agcttctata atcggataag
      361 acttggcgag ggatttccat ccttcgcctt tttttggggg ggatgccgcc ggggtgagtg
      421 gggtacaagg ggcaataagg ggcgggggga ggcgttgtag gtcacatggt gcgtgtggcc
      481 atattgttat taggggcctt gtttttggcg gggtccggag tggcggggcc tggcggcccg
      541 ggtggcaggg gtgtgcctgg gaagtccggg aagtccggtg ggtcagttgg ggcaggcggg
      601 ccgggtaagt tgggtgggcc aaatggggac cgcctgatgg cgctttatcg ccgggcggac
      661 agtctttacc acctggaccg gagcacgcgg gtgacggaca gcctggcgct ggcggggttt
      721 acggtggtgg ccgatgaact ggagaaggca gggggccggg ctggaaagga tacgatgttg
      781 acgggtgcgc ttttgaagaa gggcatcctg ctcgatgccg gcggggattt tgcacgggcg
      841 cgggtcgcgt attgcagggt acttggctat catccggcag acgactcgct tgtttttgtg
      901 acgcaggtac tggtggggac ggtctattac aatatggacc gcttcgattc ggcgagttat
      961 tttcttttgc gggcggaagc cctggcgggc aggttcagcg accgggataa ccgggtccgc
     1021 ctttacaata cacttggtgc gctttactgt gataacggca atttccggca ggggaagaac
     1081 tatttcgaca atgccctgga gcttgtccgg ggtgggaaat tgtatgatac tgcggcggcg
     1141 gtgagtttac agatcaatat tgctacggct tcgttccggg tgggccagta tgccgatgcg
     1201 ctggcgatct accggcaatt gctggggtac cggccgttgc gggattatgt gtatgagaat
     1261 atgggccgcg cgtacgtggg gatggagaat taccctgcgg cgatggactg gttccggcgg
     1321 gtgaaggtgg agcagatacc ggctgtgttg aacgagatgg ccaatgcgga gttgcggctg
     1381 caccggctgg atagctgcgt gtggtatctg cgacggctgc aggaacttgc ccggatggat
     1441 gcgggggcga gggtgcggcc ggtggacatg ggcgtgaatg cttactacac cgccgaggaa
     1501 cggagtgagc ggggggatct gcgggggggc ctggaggcgc tgcagcgggc gatcgtcgtg
     1561 ttcgcgggaa atttcaataa gcgggaccct tatggcaacc cggtgagttt ttccggggcg
     1621 tttgcctatt accggctatt cgacgccctg gttcgaaagg cggagttata tgagcgatct
     1681 tatgaagcgg ggggacggaa gaacgagaac gatcttgtgg cctcgtatgc gacgtattcg
     1741 gcggcgttgt cacttttgcg ttatatcgag aagagctacg cgacggatga ggcgaagctc
     1801 tttttgaaga agaagagcgg accggtgcat gccgaggcgt tgtcggtctg tttgaggctc
     1861 gatcgattgc atccgggtgg agattacctg gagcaggctt ttcttatcag cgagcggagc
     1921 aaggcttcgg tgatcacggc caacctggag gagcaggcat ttatgggggc gccgggtgcg
     1981 gagcaggaat tgctgcaaaa ggtgaataac tgtaaatacc ggatcgcgcg gttgaatgta
     2041 aagagcgagg gggcgctgga cagtgcggga ttggctgcta ttacgcggga gaaggagggg
     2101 gaggagacgg agttgtcgcg actgcaaaaa gcattggaac aggatgggga atattaccgg
     2161 gtcaggtatg gggacgccag tccgggggtg cggaaccttc aacggcaatt ggggaggaat
     2221 gaggcgcttg tgagcctgta cgcggcgggg aatatgttgc atgtctttgt cgtgacgaag
     2281 gatgggttga ggcatgtcgg gatggattcc ctggcgggga tggagaggga tgtggagggg
     2341 tggctggaca agttgaagac gacgggcggt ggcgtgcggt ttaacggtgg agagacagga
     2401 cgcagactat atagccggct tgtacagccg atacaagtcg cagcgggcgg gcgaacggag
     2461 tgggtgatca ttcccgatgg ggtgtttgct ttgttgccgt tcgag
//
LOCUS       DATGFG010000537         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1629920, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000537 DATGFG010000000
VERSION     DATGFG010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629920"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VKQ52_10055"
     CDS             complement(<1..1024)
                     /locus_tag="VKQ52_10055"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HLZ87575.1"
                     /translation="MVEFNDIHRVYFIGIGGIGMSALARYFQFHGKEVSGYDKTATTL
                     THELEKEGIAVHYEEDLDKIPRDADLVVYTPAVPATHKELVYYRQEGKPLLKRSEVLG
                     MITRSSFNICVAGTHGKTTITTMIAHILRHSGYGCNAFLGGIAVNYNSNYWSNERNVC
                     VVEADEYDRSFLRLSPDIAVITAMDADHLDIYGTAEEVQNAFIAFSGCLKPNGLLMSK
                     EGLKRGAELRGDHHLTYGLVPGADFFASEIRIDQGDYLFRMQSADWSIDKVVLPMGGR
                     HNVENAVAAIAVAHRLGIAGDKIVEAVAAFRGVKRRFEYIIREPGRVFIDDYAHHPEE
                     LRALISG"
     gene            complement(1017..2147)
                     /gene="murG"
                     /locus_tag="VKQ52_10060"
     CDS             complement(1017..2147)
                     /gene="murG"
                     /locus_tag="VKQ52_10060"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HLZ87576.1"
                     /translation="MSRKIIIAGGGTGGHIFPAIAIAGALKQQDPDIELLFVGAQGKM
                     EMEKVPQAGYRIEGITIAGFNRSSLIKNIGLPFKLIKSFIQVNRILRSFMPDAVVGVG
                     GYSSFPVLRSAQAKGIPTFIHESNSFAGKSNILLGKKATHVFVASDGMEKFFPAGRIS
                     VVGNPVRSAIVQSRVTREEGIRFFGLDPALPTVLSTGGSLGAKGINEAVAAHLEEFGQ
                     NGLQLIWQTGKPFAEQAATLAGGKKNVWTRDFIPQMEYAFAAADIVISRSGAMSIAEL
                     CVARKPVVFVPFPFAAEDHQTVNAQNLVNKNAGLLVKDSEAKGKLVDTVLALAKDNDR
                     QAVLRTNIGRLAITDAADRIAGEILKAIASPPQATKQSRKNG"
     gene            complement(2144..3403)
                     /locus_tag="VKQ52_10065"
     CDS             complement(2144..3403)
                     /locus_tag="VKQ52_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HLZ87577.1"
                     /translation="MSNTTTIERTPFSGLRSRTKGDKVIWTAVVLLAVVSLLVVYSST
                     GLLAYKMYKGNTEVYLFKQFAFILVGVMIIYFAHQVNYTIYSKVAKILFIVSIPLLFY
                     TLFFGVKLNEGSRWIRLPIINMTFQTSDLAKLALFMYLSRLLSRKQDVIKDFRTGFIP
                     VITPVAVICLLIAPANLSTALLIGATSLMLLFIGRVSARHLLMTIGVAAIPVALLIGA
                     AVIRHKANGGTTEKPKTEGKSRLFIRVDTWISRVETFVYGGKEDNSDNNYQTNQAKIA
                     IAKGGILGLGPGNSQQRNFLPHPYSDFIFAIIIEEYGLVGGVFIVFIYLVFLLRSIRI
                     FRRCPFAFGAFLALGLSFTLVIQALANMAVNVNLFPVTGVTLPLVSMGGSSFLFTCLA
                     IGIILSVARNAESAEGAGPVNKEEVTA"
     gene            complement(3400..>3725)
                     /locus_tag="VKQ52_10070"
     CDS             complement(3400..>3725)
                     /locus_tag="VKQ52_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87578.1"
                     /translation="ESMEKPTILILGGVDKGNDYSLIRDLVKEKVKAIVCMGVDNRKI
                     HEAFQQDVPLMVNTASAEEAVKAAFHFASKGDVVLLSPACASFDLFKNYEDRGRQFKE
                     AVRDL"
BASE COUNT          780 a         1093 c         1012 g          840 t
ORIGIN      
        1 ccccgctgat cagggcccgc agctcttccg ggtgatgcgc atagtcatcg ataaagaccc
       61 gcccgggttc cctgatgata tactcgaagc gtctttttac cccgcggaag gcggcaacag
      121 cttccacgat cttatccccc gcaatgccga gacgatgagc gacggcgatc gccgcgacgg
      181 cattctccac gttgtgccgg cctcccatgg gcaggaccac cttatcgata ctccagtcgg
      241 cgctttgcat ccggaaaaga tagtcgccct ggtcgatcct gatctccgaa gcgaagaaat
      301 cggcgccggg caccagccca taggtaagat ggtgatcgcc tctcagttcg gctccccgct
      361 tcagcccttc cttgctcatc aggagcccgt tgggtttcag acagcccgag aaagcgatga
      421 aggcgttctg aacctcttcc gccgtgccgt agatgtcgag gtgatcggca tccatcgcgg
      481 tgataacagc gatatcggga ctcaaccgaa gaaagctgcg atcgtactca tcggcctcca
      541 ccacacagac attccgctca ttgctccagt agttgctgtt ataattgaca gcgatcccgc
      601 cgagaaaggc gttacagcca tagccgctat gccgcaggat gtgcgcgatc atcgtcgtga
      661 tggtcgtttt gccatgtgtc cccgccacac agatattaaa agagctacgg gtgatcattc
      721 ccagcacttc gctgcgcttc aggaggggct tgccttcctg ccggtagtaa accagttcct
      781 tgtgcgtagc gggcaccgcc ggcgtataca ccaccaggtc ggcatcacgg ggtatcttat
      841 ccaggtcttc ctcatagtgg acggcgattc cttccttctc caattcatgg gtgagcgtgg
      901 tagccgtttt gtcataacca ctcacctcct tcccgtgaaa ttggaagtac ctggccagcg
      961 cgctcatgcc tattccgccg atgccaatga aataaaccct gtgtatatcg ttgaattcaa
     1021 ccattttttc tgctctgttt cgttgcctgt ggcggtgagg cgatcgcttt tagtatctcc
     1081 cccgcgatac ggtcggccgc atcggtgatc gccagccggc cgatatttgt tctcaacacc
     1141 gcctgccggt cgttatcttt tgccaacgcc agtaccgtgt ccactaactt cccctttgcc
     1201 tcgctatctt ttaccaggag accggcattc ttattcacca ggttttgggc gttcaccgtc
     1261 tggtggtctt cggctgcaaa aggaaacggc acaaagacaa ccggctttct ggctacacac
     1321 aattccgcga tcgacatggc ccccgaccgg gagatcacga tatccgccgc ggcaaaggca
     1381 tattccatct gggggataaa atcccgggtc cagacattct tctttcctcc cgcaagggtc
     1441 gcggcctgtt cggcaaacgg tttaccggtc tgccagataa gctgcagtcc gttctgccca
     1501 aattcctcga gatgggcggc gacggcctcg ttgatgcctt ttgcgccgag tgagccgccg
     1561 gtggaaagaa ccgtgggcag ggccgggtcc agtccaaaga accggatgcc ttcttctctc
     1621 gtgaccctgc tctgcacgat agcgctgcga acaggattcc ccacgacgct gatccggccg
     1681 gcagggaaga atttctccat gccgtcgctc gcgacgaaca catgcgtcgc cttcttcccc
     1741 aataagatat tgctcttccc cgcgaaagag ttgctttcgt ggatgaaggt ggggattcct
     1801 tttgcctgtg ccgagcgcag gacggggaag ctcgaatatc cgcccacccc taccacggca
     1861 tcgggcataa aggaccgcag gatgcggttc acctggataa agctttttat cagtttaaaa
     1921 ggaagaccga tattttttat caaagaactt ctgttgaacc cggcgatggt gatcccttcg
     1981 atccggtaac cggcctgggg aaccttctcc atctccatct taccctgtgc gccgacaaac
     2041 agcagttcga tgtcggggtc ctgttgtttc agcgccccgg cgatcgcgat ggcagggaag
     2101 atatgcccgc cggtgccacc gcccgctatg atgattttcc tgctcaagcc gttacctcct
     2161 ctttgtttac cggccccgcg ccttccgcgg attccgcatt tcgcgccacg ctcaggatga
     2221 tgccgatggc cagacaggtg aacaggaaag agctgccgcc catgctcacc aggggtaagg
     2281 tcaccccggt gacgggaaac aggttgacat tcaccgccat attggccagc gcctggatca
     2341 ccagggtgaa gctgagccca agggcgagaa aggccccaaa ggcaaagggg cagcgccgga
     2401 agatgcggat gctgcgcaac aggaacacga ggtaaataaa tacgataaat acaccaccca
     2461 ccaatccgta ttcttcgatg atgatggcga agatgaaatc cgaataaggg tgcggaagaa
     2521 aattccgttg ctggctgttg ccgggcccca gcccgagaat accacccttg gcgatggcga
     2581 tcttggcctg gttggtctgg taattattat cgctgttgtc ttcctttccg ccgtagacaa
     2641 aggtctccac acggctgatc caggtatcca cccggatgaa aagccggctt ttgccttctg
     2701 tctttggttt ctccgtcgtg ccgccattcg ccttgtgccg gataacggcc gcaccgatga
     2761 gcagggcgac gggtatggcg gccacgccga tggtcatcag cagatgcctg gcgctgaccc
     2821 ggccgatgaa caggagcatc aggctggtgg cgccgatgag cagggcggtg gagagattgg
     2881 ccggtgcgat cagcagacag atgacggcca ccggggtgat cacggggata aacccggtac
     2941 gaaagtcctt gataacatcc tgtttcctgc tcagcagcct gctcaggtac atgaacaggg
     3001 ccagcttcgc caggtcggac gtttgaaagg tcatattgat gatcggcagg cggatccacc
     3061 ggcttccttc gttgagcttt accccgaaga acagggtata gaacaacagg gggatcgaga
     3121 cgatgaagag gatcttcgcc accttggaat aaatagtata gttcacctgg tgggcgaagt
     3181 agatgatcat gacgccgacc aggatgaatg cgaattgttt gaacaggtag acctcggtgt
     3241 tgcccttgta cattttatag gccagcaaac cggtggagct atacacgacc agcaaggaca
     3301 cgacggcgag caggacgact gccgtccaga tcaccttgtc tccctttgtc cgggaccgga
     3361 gtccgctaaa gggtgttctt tctatcgtcg tggtattgct catagatcgc ggacagcctc
     3421 cttgaactgt cttcccctgt cctcgtaatt tttaaacaga tcgaaactcg cgcaggccgg
     3481 gctcagcaaa accacgtcgc ccttggatgc gaaatggaaa gcggctttta cggcttcctc
     3541 cgcgctggct gtattgacca tcagtgggac atcctgctgg aaggcctcgt ggatcttgcg
     3601 gttgtccaca cccatgcaga cgatggcctt taccttttcc ttcaccaggt cgcggatcag
     3661 cgaatagtcg ttccccttgt ccacgccgcc gaggatcagg atggtcggct tctccatgct
     3721 ttcca
//
LOCUS       DATGFG010000538         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1169047, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000538 DATGFG010000000
VERSION     DATGFG010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169047"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VKQ52_10075"
     CDS             <1..743
                     /locus_tag="VKQ52_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HLZ87579.1"
                     /translation="NLFTTLRMTATNDVYLDTLRAEMAGGKLTAQAHIDGTDPKKIYL
                     YSTLGVEDMNIEKLMLKLDNFGEDVVINKNVQGIISGQIKSTFLIHPDFTPQIDQSQA
                     EMDLEILNGVLTNFTPMQAMSSYFADKNLMKVRFDTLRNTLTFKNGALYIPDMNINSS
                     LGFMEISGKQSMNMQMEYYIRIPLKLVTQAGFHKLFGKKQEEVDPNQVDAIEYRDKDK
                     HVHFINLKISGTPDAYKVGLGKAKKNVG"
     gene            complement(781..1302)
                     /locus_tag="VKQ52_10080"
     CDS             complement(781..1302)
                     /locus_tag="VKQ52_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87580.1"
                     /translation="MATLTDEQVDFIAEDIRAHGILLEDLRDNLLDHICILVEQGLAE
                     GGDFAGLYAMIIPTFYKKELYELEEEALFLASLKGPRLLLSRGRFFQLSFGLFLTPYL
                     FYGLGDFFHLLPVAYDVLPMVILRWTLVGSVWPLICLLVIYLTPEGLDPLVPRHAKVL
                     LGAKPFIRVLPGS"
     gene            complement(1303..1644)
                     /locus_tag="VKQ52_10085"
     CDS             complement(1303..1644)
                     /locus_tag="VKQ52_10085"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLZ87581.1"
                     /translation="MVYSTELLKGTLKTIVLKLLADNKKMYGYEITQRVKEITSDRIQ
                     ITEGALYPTLHALEADKLVTTEVEYIGKRVRKYYSLSSTGKAKAKEKVNELASFIQTM
                     TLLLDIKPKPI"
     gene            1738..>2292
                     /locus_tag="VKQ52_10090"
     CDS             1738..>2292
                     /locus_tag="VKQ52_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87582.1"
                     /translation="MLFRFSSIPSGPAGPGLLVQGDTIFARHKVETKAAKRTIFLAEH
                     LLVFVVQGTKFLHLPGQTITVAPNSVVLLKKGIYVMAEYIGEGLSLESMMVFLPVGAI
                     KQFALEQGWQQPSAAPDCSCVVIPTNELLDSFKTQYLHYFGKSFPGLEQLLAGKLREL
                     LLLLLATSQRETIIRFLQSAMDEEP"
BASE COUNT          567 a          658 c          570 g          497 t
ORIGIN      
        1 agaacctctt cacgacgttg cggatgacgg ccaccaatga tgtctacctg gacacgttga
       61 gggcggagat ggccggcgga aaactcaccg cccaggcgca tatcgacggc accgacccga
      121 aaaagatcta cctctatagc acccttggcg tcgaagacat gaatatcgaa aagctgatgc
      181 tcaagctcga caattttggc gaggacgtag tgatcaataa gaacgtacag ggcatcatca
      241 gcggccagat aaagagcacc ttcctgatcc acccggattt caccccgcag atcgatcagt
      301 cacaggcgga gatggacctc gagatattaa acggggtatt gacgaatttc acgcccatgc
      361 aggccatgtc ttcctacttc gcggataaga acctgatgaa ggtgcgtttc gatacccttc
      421 gcaacacgct taccttcaag aatggggcgc tctatatccc cgatatgaac atcaattcct
      481 cccttggttt tatggagatc tccggcaagc agtcgatgaa catgcagatg gaatactata
      541 ttcgcattcc cctgaaactg gtgacccagg ccggtttcca caagctcttc ggcaagaagc
      601 aggaagaggt ggaccccaac caggtagacg ccatcgagta ccgcgacaag gataaacacg
      661 tccatttcat caacctgaag ataagcggca ccccggatgc ctacaaggtg gggctgggca
      721 aggcaaaaaa gaacgtgggc tgatcatccc gcaatgcctg gaacaagtgt cgatcatccc
      781 ctatgatccc ggcaataccc ggataaaggg cttcgcgccc aacaggacct tcgcatggcg
      841 gggaaccagc ggatcgagcc cctccggtgt aagatagatc accagcaggc agatcagcgg
      901 ccagacagaa ccgaccaggg tccagcgcag gatcaccatc ggcaggacat cataggccac
      961 cggtagcagg tgaaaaaagt cgcccagccc ataaaaaaga tagggcgtca ggaacaatcc
     1021 aaatgacagt tgaaagaacc ggccccggct cagcaacagc cgcggcccct tcagggaagc
     1081 caggaacaac gcctcctcct ccagttcata cagctccttt ttataaaagg tcggaatgat
     1141 catcgcgtac aatccggcaa aatccccacc ctccgccaat ccctgttcga caaggatgca
     1201 gatatgatcc agcaggttgt cccgcagatc ctccagcagg atcccatgcg cacggatatc
     1261 ctccgctata aagtctacct gttcatcggt taaggtcgcc atttaaatgg gttttggttt
     1321 tatgtcgagc aacagggtca ttgtctggat aaacgacgcc agctcattca ccttctcctt
     1381 tgccttcgcc ttaccggtcg agctcaggct ataatatttc cgcacccgct tgccgatata
     1441 ctccacctcc gtcgtcacca gcttatccgc ctccagcgca tgcaaggtcg gataaagcgc
     1501 accctcggtg atctgtatcc tgtcgctggt gatctccttc acccgctgcg tgatctcgta
     1561 cccgtacatt ttcttgttgt cggccagcag ttttaaaacg atggttttaa gcgtgccctt
     1621 tagtaattcg gttgaataga ccatgggcca atatacataa aattccaatg tattggagtc
     1681 acaggggaga aaaaatgcta tccgtatcct tcttctggct acctttgcgc ttcttctatg
     1741 ttatttcgtt tttcttccat cccttccggc cctgcgggcc cgggcctgct cgttcaggga
     1801 gataccattt ttgcccgtca taaggtggag acaaaggcgg ccaagaggac catattcctc
     1861 gcggaacatc tcctggtgtt cgtcgtgcag ggaacaaaat tcctgcacct gcccggacag
     1921 acgatcaccg tggcccccaa cagcgtggta ttgctgaaaa aaggcatcta tgtcatggcg
     1981 gaatatatcg gggaggggct ctccctggaa tcgatgatgg tcttcctgcc ggtgggggcg
     2041 ataaagcaat tcgccctgga gcagggctgg caacagccat cggcggcgcc cgactgttcc
     2101 tgtgtcgtca tcccaaccaa cgagctgctg gacagtttta agacccagta ccttcactac
     2161 ttcggcaaat cctttcccgg attggaacag ctgctggcgg gaaagctccg cgaactgctg
     2221 ctcctgttgc tggccaccag ccagcgcgag acgatcattc gttttctgca atcggcgatg
     2281 gatgaagaac cc
//
LOCUS       DATGFG010000539         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_16152, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000539 DATGFG010000000
VERSION     DATGFG010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16152"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VKQ52_10095"
     CDS             complement(<1..222)
                     /locus_tag="VKQ52_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87583.1"
                     /translation="MKRKDFTYTLVIIAAVIISLCFPNYFVEIGGYKLAGLITPLLQI
                     IMFGMGTSMSIHDFAGVIKTPKGVIIGVFS"
     gene            complement(238..1629)
                     /locus_tag="VKQ52_10100"
     CDS             complement(238..1629)
                     /locus_tag="VKQ52_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLZ87584.1"
                     /translation="MKNSRRNFLRNTALGGLAVGGLANLSFEDRVAHTTRGVRRASAP
                     ADLKITDMRYAVVQNVGRTAIIRIDTNQGIYGLGEVRDGADERYALMLKSRILGQNPC
                     NVEMIFKMIRQFGGPSRQAGGVCGVEMALWDLCGKAYNVPAWQLLGGRYRDKIRLYAD
                     TPEAKSPEEQRKLIKYRLEEQGYTWLKMDVGIGEIKNIPGALVNADYWGPVLNQWQGD
                     YMSYANTEHPFTQIQITDKGFEELTRIVDNVRSMVGYGIPLSTDHYGHFDLNNGIRLG
                     RALEKYRLAWMEDIVSWERTDQWKTITDALETPTLTGEDIYLLKNFKPLIDIHAVDII
                     HPDLVSSGGLLETKHIGDYAEEHGIAMAMHQAGTPVSFMANVHCAAATQNFLALEHHS
                     IDVPWWESLVKTTDGRALITKGFANIPLTAPGLGIELNDEEVKKHLAAKDKSYFAPTP
                     EWNDLRSHDRIYS"
     gene            complement(1641..2579)
                     /locus_tag="VKQ52_10105"
     CDS             complement(1641..2579)
                     /locus_tag="VKQ52_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87585.1"
                     /translation="MYMNKKLLFSALFVFGCYCSEAQQVGMSPEYIKALTSQWKGERF
                     SDGRPKVSDDLLERLKPLSLEDIWNVLRNKGYLNQFEGNWQVVNPDSVMTGRVVTAQY
                     MPLRPDYADLIKQQGKAEGRGQHGGTNSWPIDILVDGDVYVADSYEKMADGTLIGDNL
                     GNSIYAHSHRGVIFYGSVRDMAGLSEIKGFNGWVKGSDPSYIQQMMLSSINAPIRIGR
                     ATVLPGDVVLANKWGVIFIPAYLCQEVVITSEVTELRDEFGHQRLREKKYLTGQIDSE
                     WTEDIKKDFLDWLNHYPGKLPMTKKELDDYLKERNY"
BASE COUNT          677 a          896 c          760 g          748 t
ORIGIN      
        1 gctgaaaaca ccgatgataa cgcccttcgg cgtttttatc acccccgcaa aatcatggat
       61 actcatggag gtacccatcc cgaacatgat gatctgtaac aacggcgtga tcaggcccgc
      121 taatttatat cccccgatct cgacaaaata gttggggaag caaagagaga tgatcaccgc
      181 cgcaatgata accagggtat aggtaaaatc tttccgtttc aaaatgttta ttgtttatta
      241 gctataaatc ctgtcgtggc tacgcaggtc attccattcc ggcgtgggag caaaataact
      301 cttgtccttt gccgcaagat gtttttttac ttcttcgtca ttgagctcga tcccgagccc
      361 gggagcggtc agcggaatat tggcgaatcc cttcgtgatc agggcgcggc cgtccgtcgt
      421 tttgaccagg ctttcccacc agggtacatc tatcgaatga tgttccaggg caaggaagtt
      481 ctgtgtcgcc gcggcgcagt gtacattcgc cataaagctg acgggcgttc ccgcctggtg
      541 catggccatg gcgatgccat gttcttcggc atagtccccg atgtgttttg tctccagcag
      601 cccgccgctg ctcaccaggt cgggatggat gatgtcgacg gcgtgaatat cgatcagcgg
      661 tttgaaattc ttcagcaggt aaatatcttc cccggtgagc gtaggcgtct cgagggcgtc
      721 ggtgatcgtc ttccactgat cggtgcgttc ccaggatacg atatcctcca tccacgccag
      781 ccggtatttc tccagcgccc ttccaagacg gatgccgttg ttcaggtcga aatgtccata
      841 gtggtccgtc gataacggta tgccgtagcc gaccatgctc cggacattgt ccacgatcct
      901 ggtcagttcc tcaaacccct tgtcggtgat ctggatctgg gtaaacggat gttcggtatt
      961 cgcataggac atataatctc cctgccactg gttgaggacg ggcccccagt aatccgcatt
     1021 gacgagggcg cccgggatat ttttgatctc gccgatccct acatccatct tcagccaggt
     1081 gtagccctgc tcctccagcc tgtatttgat caacttccgt tgctcttccg gggatttcgc
     1141 ctcgggagta tccgcataca ggcggatctt atcccggtac cgcccgccca gcagctgcca
     1201 ggcagggaca ttataggcct tgccgcaaag atcccaaagc gccatctcca cgccgcagac
     1261 ccctccggcc tggcggctcg gccccccaaa ctgccggatc atcttaaaga tcatctccac
     1321 attgcagggg ttttgcccga ggatccggct cttcagcatc agcgcatacc gctcgtccgc
     1381 cccatccctt acttctccaa ggccatagat cccctggttg gtgtcgatgc ggatgatggc
     1441 cgtacgcccc acattctgaa cgacggcata ccgcatatcc gtaattttca gatcggcggg
     1501 cgccgaagcc cggcgaacgc cacgggtcgt atgcgcaacc cgatcttcga atgaaagatt
     1561 ggccagcccg cccaccgcaa ggccgcccaa cgcagtgttc cgtaaaaagt tccttctgct
     1621 atttttcata tggcttgctt ttaataattg cgctccttca gatagtcatc gagttctttt
     1681 tttgtcatcg gcaatttccc cgggtaatgg ttcagccagt cgaggaaatc cttctttata
     1741 tcttccgtcc actcgctgtc gatctggccg gtaagatatt tcttctcccg cagccgctgg
     1801 tgcccgaact catcgcgcaa ttcggtcacc tcggaggtga tcactacttc ctggcagaga
     1861 taggcgggga taaagatgac cccccattta ttggccagca ccacatcccc gggaagcacg
     1921 gtggccctgc cgatccggat cggggcattg atggaggaga gcatcatctg ctggatatag
     1981 gaagggtccg atccctttac ccagccgtta aaacccttta tctcgctcag cccggccatg
     2041 tcccggacac tgccataaaa gatgacgccg cggtgcgagt gcgcatagat ggaatttccc
     2101 aggttatccc cgataagcgt accatcggcc atcttctcat agctatcggc gacatagaca
     2161 tccccatcga caaggatatc gatgggccag gaattggtgc cgccatgctg cccgcggcct
     2221 tcggcctttc cctgctgctt gatcaggtcg gcgtagtcgg gacgcaaggg catatactgc
     2281 gccgttacca cccggcccgt catgacgctg tcggggttca ccacctgcca gttgccttcg
     2341 aactgattca aatatccctt attccgcagc acgttccaga tatcttccag cgataggggc
     2401 tttagccgtt caagcagatc atccgacacc ttcggccggc cgtcggagaa ccgctccccc
     2461 ttccactggg aagtgagcgc cttgatatac tccggggaca tgcccacctg ctgggcctca
     2521 ctgcaatagc agccaaaaac gaagagcgcg gaaaaaagca gctttttgtt catatacata
     2581 gcgattgttt acgtgttttg ttcggataaa attcctcctg gtgtccgtca tgacgaataa
     2641 atttatttta gcgcatatgc gcgcatgtga gagcgcgctg atcacttctg aaggagagtg
     2701 gcatcccggg gaaggaacat cgtcgagttg tcgcctggct cacgaattta atataaatcg
     2761 ttgatacgca ggcaaaattc cgcaaatatg aaaagaacaa gaaacggata aaatagtact
     2821 atttaatctc cgccttagtt tggaatttta gcctgccatt cacctcgact tttccattca
     2881 aaactaagtg cgcgcccccc tcccctatcg ccgccctgcc ggcgcactaa cggctattct
     2941 aaccattata aatgaaaaaa aaatgaacaa actaacattg cttgagcatg ggctgcgcaa
     3001 aagctggctg ctgaccttgc tgctggcggc actggccaat gtcgctgttc ttgcacaaac
     3061 ccggccgatc acgggaaagg t
//
LOCUS       DATGFG010000540         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_693687, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000540 DATGFG010000000
VERSION     DATGFG010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693687"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VKQ52_10110"
     CDS             complement(<1..1152)
                     /locus_tag="VKQ52_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87586.1"
                     /translation="MRDNKIFGPEGGADGKGPGDGGMFVGRGPGVEEFAGGGPGVAWY
                     FRMAWRDSRRNRGRLLLFISSIVLGIAALVSTLSFGHNLREGIDDQAKELVGADLVFG
                     AYHPVSAKVLAQADSAPNRHSEERSFASMIYFVKSGQSRLVQVRALNGDYPFYGSLVT
                     TPAAAGETFRGKQMALIDKTLLLQYGGQVGDSVRIGNLSFAIAGSLDQAPGRNELSTT
                     MAPPVYIPLRYLADAGLVRMGSRVDYRYYYQYPRSDVRKLMTVLGPRLEKADLYYDTV
                     DTRKRKMSRGFGDLTQFLTLISFIALLLGCVGVGSSVNIYIKEKIADIAVLRCLGLKS
                     RQAFFIYLIQVLGIGLIGSVAGAALGVAIQQVLPGVLKDLLPFQTSFRIS"
     gene            complement(1139..1819)
                     /locus_tag="VKQ52_10115"
     CDS             complement(1139..1819)
                     /locus_tag="VKQ52_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ87587.1"
                     /translation="METILTVSNVSKTYHLAGKTLHVLNDVSFSVEAGSTLAIVGPSG
                     SGKTTLLGLSAGLDRSTSGSVELNNIPLGELSEDERAAVRNRYVGFIFQNFQLMPTLT
                     ALENVMVPMELKREKHARTRAKELLDTVGLGDRLHHYPVQLSGGEQQRVSLARAFANQ
                     PRILFADEPTGNLDAETSEKITQLLFELNRQAGTTLVLVTHNTELAASTQRIIRLAGG
                     HIIADERQ"
     gene            1900..2538
                     /locus_tag="VKQ52_10120"
     CDS             1900..2538
                     /locus_tag="VKQ52_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HLZ87588.1"
                     /translation="MHNRPHNTRAYTSILIMVLLLVTASFQPRTQKHIVFFGDSLTAG
                     YGVSPGQGFPELIQHRLDSLHLPYQATNAGVSGETSSGGKNRIAWVLRQPVDIFVLEL
                     GANDGLRGIPVAGTTANLQAIIDAVKARYPSARIILAGMQVPPSMGSTYATAFRNLFP
                     ALAAKNKISLIPFLLQNVGGIPQLNQRDGIHPNPAGHRIVASNVWQTLKPLL"
     gene            2733..2912
                     /locus_tag="VKQ52_10125"
     CDS             2733..2912
                     /locus_tag="VKQ52_10125"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLZ87589.1"
                     /translation="MKSSELHRRIRKNGWNHIRTDGSHYIYEKEGRTYPVPYHGAKEI
                     GKGLEKKIIKEMQLK"
     gene            2940..3338
                     /locus_tag="VKQ52_10130"
     CDS             2940..3338
                     /locus_tag="VKQ52_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87590.1"
                     /translation="MKLVKVIIEKTKNMYSSYAENVSGIYGGGDTVEEAKNSILDAIR
                     LLKEHNDKKHIPAILRGKYTIVYKYDAESFLKHYKGIFTNSALERITGINQKQFHHYA
                     SGLKKPRRAQAKKIESALHRLGNELLSIEL"
BASE COUNT          853 a         1146 c          731 g          657 t
ORIGIN      
        1 cgagatgcga aagctcgtct gaaagggcag gaggtctttc aatacgcccg gcaacacctg
       61 ctggatcgct accccaagcg ccgcccccgc caccgatccg atcagcccga tccccagcac
      121 ctggatcagg taaataaaga aagcctgcct cgactttaac ccaagacaac gaagcacggc
      181 gatatcggct attttctctt tgatataaat attcacgctg ctgcctacgc cgacacaccc
      241 caacagtaac gcgatgaagc tgatcaacgt caggaattgc gtcagatcgc caaatcccct
      301 gctcattttt ctctttcgcg tatcgacggt atcatagtac aggtccgcct tctccagcct
      361 tgggccaaga acggtcatca acttccggac gtcgcttcgt ggatattgat agtagtagcg
      421 gtaatcgacc ctgcttccca tccgcacaag ccccgcgtca gccaggtaac gcagcgggat
      481 ataaacaggc ggcgccatcg tcgtcgacaa ttcattgcgg cccggcgcct ggtcgaggct
      541 ccccgcgatg gcaaaactca ggttgccgat ccgcacgctg tcccccacct gaccgccgta
      601 ctgtaacagc agggtcttat cgataagcgc catctgcttg ccccggaagg tctcgcccgc
      661 cgctgccggc gtcgtgacaa gagacccgta aaaaggatag tcgccgttca acgcgcgcac
      721 ctgcaccagc cggctctgcc cgctcttgac aaaatagatc atggaagcaa agcttcgctc
      781 ttccgaatgc ctgttcggcg cggaatccgc ctgcgccagc actttcgccg acacgggatg
      841 ataggcgcca aacaccaggt cggccccgac cagttccttc gcctggtcgt cgatcccctc
      901 gcgcaggtta tgcccaaacg aaagcgtcga caccagcgcc gcaatcccca atacgatgga
      961 tgaaataaaa agcaataacc gcccccggtt ccgccggcta tcccgccagg ccatccggaa
     1021 ataccacgcc acgcccggcc cgcctccggc aaactcctct acccccgggc cacgcccaac
     1081 aaacatcccc ccgtcgcccg gccccttccc atcggcccca ccttccgggc caaatatttt
     1141 attgtctctc atcggcaatg atatggcctc ctgcgagccg gataatgcgt tgggtactgg
     1201 ccgccaattc ggtattatgc gtaacgagga ccagcgtcgt ccccgcctgg cggttaagct
     1261 caaaaagaag ctgcgtgatc ttctcgctgg tctccgcatc aagattgccc gtgggctcgt
     1321 cggcaaacaa aatacgcggc tgattcgcaa aagcccgcgc cagcgacacc cgctgctgct
     1381 ccccacccga taactgaacg gggtagtgat gcaaccggtc ccccagccca accgtatcca
     1441 gcaattcctt tgccctcgtt cgcgcatgct tctcccgctt cagctccatc ggaaccatca
     1501 cattctccaa cgccgtcagg gtgggcatca actggaaatt ctggaaaata aatccgacat
     1561 accggtttcg gacagccgcc cgctcatcct cgctgagctc gccaagcggg atattattca
     1621 actccactga acccgatgtg ctgcggtcga ggcctgcgct caatccaagc agggtcgtct
     1681 tcccgctgcc ggaggggccg acgatggcaa gggtcgatcc cgcttccacg gagaagctca
     1741 catcattgag aacatggagg gttttgccgg ctaaatggta ggtcttgctg acattgctga
     1801 cggtaaggat ggtttccaca agcataattt acaactatat ccgtaaaatt gttgttcaat
     1861 aggtattctc cgtccagttt agacgccaaa gccgccaaaa tgcacaaccg tcctcataac
     1921 acccgggcat acacctccat cctgatcatg gtcctgttgc tcgtcaccgc ctccttccag
     1981 ccgcgaaccc aaaaacacat cgtcttcttc ggcgacagcc tcactgccgg ttatggcgtg
     2041 tcgccgggtc agggcttccc cgaactcatc cagcatcgtc tcgactccct ccaccttcct
     2101 taccaggcca ccaatgccgg cgtcagcggc gaaacctcct cgggcggcaa aaaccgcatc
     2161 gcctgggtcc tccgccaacc cgtggacatc ttcgtcctcg aactcggcgc caacgacggc
     2221 ctccgcggga tccccgtggc cggcaccacc gccaacctcc aggcgatcat cgacgcggta
     2281 aaggcccgct atccctcggc ccggatcatt ctcgccggca tgcaggtccc cccttccatg
     2341 ggctccacct acgccaccgc cttccgcaac ctcttccccg ccctcgccgc caaaaacaag
     2401 atcagcctca tccccttcct cctccaaaac gtcggcggca tcccccaact caaccaacgc
     2461 gacggcatcc accccaaccc cgccggccac cgcatcgtcg cctccaacgt ctggcaaacc
     2521 ctcaaacccc tgctctgacc tacccccaaa ttctcttgac actgtcagga aaattccaaa
     2581 tacctgaaaa tcaaaaaagt ataattttcc agacactgtc tggaaaatcc ttaaacttcc
     2641 cccgtctcta attccccaat cttttattac aaatattctc tccaaaaggt agcattttta
     2701 ctacctttac atcgccagca aacctcatcc ctatgaagtc gagcgaactg caccgaagga
     2761 tcagaaaaaa tggatggaac catatccgca cagacggaag ccactatata tatgagaagg
     2821 agggacgcac ataccccgtg ccctatcatg gcgcaaaaga aataggcaag ggattagaaa
     2881 agaagatcat caaggaaatg caactaaaat aacctcacca ctcgcaaaaa caaacaaaaa
     2941 tgaagctcgt aaaagtgatc attgagaaaa caaaaaatat gtactcctcc tacgccgaaa
     3001 acgtctcagg catctatggc ggcggcgata ctgtcgagga agcaaagaac tcgatcctgg
     3061 atgcgattcg tctcctgaaa gaacacaacg acaaaaaaca catcccagct attttgcgag
     3121 gcaaatacac tatcgtctac aaatacgacg cagaaagctt cttaaaacac tataagggca
     3181 tatttacaaa ctccgccctg gaacgtatca ctggcatcaa ccagaagcaa ttccatcatt
     3241 acgcatcggg cctgaaaaaa ccacgccgcg cacaggcaaa aaagatagaa tccgccttgc
     3301 acaggctcgg caatgaactt ttatccatcg agctttaact attcgtctaa ccccaacccc
     3361 caacctaacc accccaaccc cgccaac
//
LOCUS       DATGFG010000541         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1432788, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000541 DATGFG010000000
VERSION     DATGFG010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432788"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VKQ52_10135"
     CDS             <1..1131
                     /locus_tag="VKQ52_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87591.1"
                     /translation="RNLSLKTSGASNGQTIAGVMATGAHGSAFDVGAVQDYVVGLHII
                     VSPTRHIWLERASYPVASTTFLQSLQTERIQDDDLFYSALVSFGSFGIIHGAMIETEP
                     LFLLESYMSRMPYDATLQQLMQTLDFSNARLPNGNERPYHFAVIINPYDIGNGAYVTT
                     MYKRPYVPGYTPPQPNKEGIGPGDDAPCFIGRLTQAIPALVPTLVNKLLAGALTPFAK
                     QMGTLAETFDNTDLHGKLLSAAIGIPISYVGQAISLLLDINKTSGPFAGLFACRFVKK
                     SMAKLAFTHFDYTCVLELDAAFSDETYAFYTAVWKGFEDAKIPFAFHWGKINELNFDR
                     LTTMYGDNVQTWIAARNKLLDADTRQVFTNATLRAWGLDKSL"
     gene            1133..>2210
                     /locus_tag="VKQ52_10140"
     CDS             1133..>2210
                     /locus_tag="VKQ52_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87592.1"
                     /translation="MYKLLIPLTLALAARTATAQTSTPQIPRVRVTTALTPDSLIAQL
                     KMINAAVAGPSPAAGKHLLLSNRALNVFLSEAAGYYLSTPNDLTLYKNSVIANSAEGT
                     FALYHNMREPAGIDSSLRRFLSVGTQANVADAFNASSSGRPYNNQFGFLLKQTWIAKP
                     RVTATPGEIRAMDALRAGILHSLEGDIRRKAAAGEAALNAIDTARDIPGQDLASAKDI
                     ARQKFNADLQLETTFEFAYRQAEALARSFGYRVVAFNWTSISLYLPLVTENFQVAASP
                     GAVLSSRHAYPVHFNLTHTRLWEGGTFGRIFLTLAGDCSLNNSRDGYILTKSGNNYIG
                     DYNSFITPAVKGQLIYIPKDSHIGI"
BASE COUNT          488 a          744 c          529 g          449 t
ORIGIN      
        1 cggaacctct cgctaaagac ctccggcgcc agcaacggcc agaccatcgc cggcgtaatg
       61 gccaccggcg ctcatgggtc cgccttcgac gtcggcgccg tacaggatta tgtcgtcggc
      121 ctgcatatca tcgtcagccc cacccgtcat atctggctgg aacgggcctc ctaccccgtc
      181 gcttccacta cctttctcca aagcctccag accgaacgca tccaggatga cgatcttttc
      241 tactcggcac tcgtcagttt cggcagcttc ggtatcatcc atggcgcgat gatcgagacc
      301 gaaccactct tcctgctgga aagctatatg tcgcggatgc cctacgacgc caccctgcaa
      361 caactcatgc agaccctcga tttcagcaat gccagactgc cgaacgggaa cgaacgaccc
      421 tatcatttcg ccgtcattat caatccctac gatataggca atggcgccta tgtcacgaca
      481 atgtataaac gcccatatgt tccgggctat accccgccac agcccaacaa ggaagggatc
      541 gggccggggg atgacgcccc ttgtttcatc ggcaggctta cccaggccat cccggcactg
      601 gtgcccaccc tcgtcaacaa gctgctggcg ggcgccctta ccccttttgc caaacaaatg
      661 ggtacgctgg ccgagacctt tgacaatacc gacctccacg ggaaactgct gagcgccgcc
      721 atcggcatac cgatcagtta tgtcggccag gcgataagtc tcctgctgga catcaataag
      781 acaagtggtc cattcgccgg gctcttcgct tgccggttcg taaagaagtc catggccaaa
      841 ctggccttca cccattttga ttatacctgt gtgctggaac tggacgccgc cttctccgac
      901 gagacctatg ccttctatac cgccgtatgg aaaggatttg aagacgcaaa gatccccttt
      961 gcctttcatt gggggaagat aaacgaattg aatttcgacc gcctgacaac catgtacggc
     1021 gataatgtcc agacctggat cgccgcccgg aacaagctgc tggacgccga cacccggcag
     1081 gtattcacga atgccaccct gcgtgcatgg ggattggaca agtctttata gtatgtataa
     1141 actgctgata ccgctgacac ttgcgcttgc ggcccgcaca gcaacggctc aaacctcgac
     1201 tccccagatc cccagggtac gggtcacaac ggccctcact cccgacagcc ttatcgccca
     1261 actgaagatg atcaacgccg ccgtggcggg cccatccccc gccgccggta aacacctgct
     1321 tctttccaat cgcgcgctga acgtcttcct gtcggaggcg gcagggtact atctttccac
     1381 tcccaacgat cttaccctct ataagaattc cgtcatcgcc aattcggccg aaggaacatt
     1441 cgccctctat cataacatgc gtgagccggc cggcatcgac tccagcctcc ggcgattcct
     1501 tagcgtcggc acccaggcca atgtcgccga tgccttcaac gcctcctcgt ccggacggcc
     1561 ctataacaat caatttgggt tcctgctgaa acagacctgg atcgccaagc cccgcgtcac
     1621 ggccacaccc ggtgagatca gggcgatgga cgccctgcgc gccggcatcc tccattcgtt
     1681 ggaaggagac atccgccgca aagccgcggc cggggaagcg gcgctaaacg ccatcgatac
     1741 cgctagagat atcccgggac aggatctcgc ctcggccaaa gacatcgccc ggcagaaatt
     1801 caacgccgac ctgcaattgg aaaccacctt cgaattcgcc tatcgccagg cggaagccct
     1861 ggcgcgcagc ttcggctaca gggtcgtagc ttttaactgg acaagcatca gtctctatct
     1921 tcccctggtg accgagaact ttcaggtcgc tgcgtctccc ggcgccgtcc tctccagccg
     1981 gcatgcttat cccgtacatt tcaacctcac ccatacccgc ctgtgggaag gcggtacatt
     2041 cggccgcatc ttcctcactc tggccggcga ctgctcactg aacaattccc gcgacggcta
     2101 catcctcaca aaatccggaa acaactatat cggcgattac aacagcttta ttactcccgc
     2161 cgtaaaaggg cagctcatct atattccgaa agattcgcac atcggcatca
//
LOCUS       DATGFG010000542         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1046209, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000542 DATGFG010000000
VERSION     DATGFG010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046209"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..1580)
                     /locus_tag="VKQ52_10145"
     CDS             complement(348..1580)
                     /locus_tag="VKQ52_10145"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HLZ87593.1"
                     /translation="MPKKIKVANPVVELDGDEMTRIIWKFIKDKLILPYLDIDIKYYD
                     LGVEYRDKTDDQVTVDAANAIKQYGVGIKCATITPDEARVKEFHLKQMWKSPNGTIRN
                     ILDGTVFREPIVMKNVPRLVTNWTAPIIVGRHAFGDQYRATDTVIKGKGKLTMTFTPE
                     DGSAPQTFEVFNFKGDGVALSMYNTDESIKGFARSCFNMALSKKWPLYLSTKNTILKK
                     YDGRFKDIFEDIYQKEFKQAFQEAGIVYEHRLIDDMVASALKWNGNFVWACKNYDGDV
                     QSDSVAQGFGSLGLMTSVLVTPDGKIMEAEAAHGTVTRHYRDHQAGKPTSTNPIASIF
                     AWTRGLAFRGKLDGNKELIDFANALEAVCVETVESGLMTKDLAICVHGNKVEHGKHFL
                     YTEEFLDAIDKNLQKKLN"
     gene            1693..2316
                     /locus_tag="VKQ52_10150"
     CDS             1693..2316
                     /locus_tag="VKQ52_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ87594.1"
                     /translation="MLELLRQYVSGYVALTNEEFTLLAERIVVRTFDRRVQLLAVGER
                     EEYMNFVVKGLVRMYFHRGKTEVITNIAKEGELISSSSSFLSGMPSHYFVETLEPATL
                     LSITRADLDEIYRQSVLIERLGRLMTTQFVMQKEEWEHECMRLDTRERFTRFVEKNPD
                     LVSRVPQKYLASYLNMKPETFSRLKNLVKKRPAAVKSPSRTEFGSRN"
     gene            2324..>2847
                     /locus_tag="VKQ52_10155"
     CDS             2324..>2847
                     /locus_tag="VKQ52_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLZ87595.1"
                     /translation="MSNAITSYKAALEELYRYLSRFTPLSKQEFNQFLPYIEVREFEK
                     KAVVTGRGDVERYLNIVAWGLVRKYLPVRNREITIQLATEGHIIHSDISFHYRSPSTS
                     IIETIEATVLFSISYDSLQEAYDLFPKAERLGRLLISDLFVKKDNRYFDQLRKNTRER
                     FLDYVRSHPQMLQRV"
BASE COUNT          650 a          690 c          762 g          745 t
ORIGIN      
        1 agaagcagga gtagtgtcct catggtagta gtagttttgg tagtgagaaa tatgaggacc
       61 taaagtaggg aagaattaca tgttatcttt cgtatatata taaagtataa aaaatagcca
      121 tttgaaatta tttgccttac agggtcattt tgtggacatt ttaagccata taatcggttt
      181 tggttgagcg ttagcaaggt ctggggtaga ttgggatcaa attaaaaatc tattaatatt
      241 atgcgctaat aaaaatgtta gtggctttcg ctgcgaaatg gctgcagaaa agcaaaaccc
      301 ctctgccgga gcaaaggggt tttaatcaag tcgaagagcc gtttggccta atttagcttt
      361 ttttgcaggt tcttatcaat cgcatcgagg aactcttcgg tgtagaggaa atgcttcccg
      421 tgttcgacct tattgccgtg tacacagatc gccaggtcct ttgtcatcag accgctttcc
      481 accgtctcca cgcatacggc ttcgagggcg ttggcaaagt cgatcagttc cttgttgccg
      541 tccagcttcc cgcggaaggc aagaccgcgg gtccatgcga agatggaggc aatcgggttt
      601 gtggaggtag gcttccccgc ctggtggtcc cggtagtggc gggtcaccgt accatgggcg
      661 gcctcggcct ccatgatctt gccgtcgggc gtgaccagca ccgaggtcat cagtcccagc
      721 gaaccaaagc cctgggcaac ggagtcgctc tgcacatcac cgtcatagtt cttacatgcc
      781 catacgaaat taccgttcca tttcagcgcg ctggccacca tgtcgtcgat aagccggtgt
      841 tcatagacga tgccggcttc ctggaaggcc tgtttgaact ctttttggta gatatcttcg
      901 aagatatcct tgaaccggcc atcgtatttc ttcaggatcg tgttctttgt cgaaagataa
      961 agcggccatt tcttgctgag cgccatatta aagcagctac gggcaaagcc tttgatggac
     1021 tcgtcggtat tgtacatgga cagggctacg ccgtcaccct tgaagttgaa cacttcgaag
     1081 gtctggggtg cactgccgtc ctcgggggta aaggtcatcg tgagtttgcc cttccccttg
     1141 ataaccgtat cggtagcccg gtactgatcg ccaaaagcat gtctgccgac gataatgggt
     1201 gccgtccaat tggtaaccag ccggggaaca ttcttcatga cgatgggctc gcgaaatacc
     1261 gttccgtcga gaatattgcg gatcgttccg ttggggctct tccacatttg cttcaggtgg
     1321 aactccttta cccgcgcctc gtcgggggtg atggttgcac atttgatgcc gacaccgtat
     1381 tgcttgatgg cgttggccgc atcgaccgtt acctggtcgt ccgtcttgtc gcggtactcg
     1441 acgccgaggt cataatattt gatatcgata tccaggtagg gcaggatcag tttgtctttg
     1501 atgaatttcc agatgatccg ggtcatttcg tccccgtcca gttcgacgac gggattggca
     1561 actttgattt ttttgggcat atttctacta tttttatcgc taaaaggata gcaaacctac
     1621 acgaattgag tcattgggca aaatcctctt tttcgtaagt ttgtgtagct ttcttcttct
     1681 taactacctg ctatgcttga attgttgcgg caatacgtat cggggtatgt agcattgacc
     1741 aacgaggagt tcacgcttct ggcggaaagg atcgtagtcc ggaccttcga ccggcgggta
     1801 cagctattgg cggtagggga gagggaggag tatatgaatt ttgtggtaaa ggggctggtc
     1861 aggatgtatt ttcaccgggg caagaccgag gtgatcacca atattgccaa ggagggggaa
     1921 ctcatcagca gttcgtcatc cttcctttcg ggtatgcctt cccactattt tgtagaaacg
     1981 ctggagccgg cgacgctgtt atcgatcacc cgcgccgatc tcgatgagat ctaccggcag
     2041 agcgtcctga tcgaacggct ggggcggctc atgaccactc aattcgtgat gcagaaggag
     2101 gaatgggaac acgagtgtat gcgcctggat acccgtgagc ggttcacccg cttcgtggag
     2161 aagaaccccg acctcgtgtc gcgggtgccg cagaagtacc tggcctctta tctgaatatg
     2221 aagcccgaga cctttagccg gttgaaaaat ctggtaaaaa agcgaccggc cgccgttaaa
     2281 tcaccgagcc ggacggagtt cggttcaaga aattaaagtc aacatgagta acgccatcac
     2341 gtcctacaag gctgctttgg aggaactcta tcgttatctc tcccgcttta ctcccctgtc
     2401 gaaacaggag ttcaaccagt tcctgccgta tatcgaggtc cgggaattcg agaaaaaagc
     2461 ggtggtcacc ggcagggggg atgtggagcg ttacctgaat atcgtcgcct gggggctggt
     2521 acggaagtac ctgccggtaa ggaaccggga gatcaccatt cagctggcaa cggaggggca
     2581 tatcatccat tcggatattt ccttccatta ccggtccccc tccacgagta tcatcgagac
     2641 catcgaagcg acggtcctct tctccatttc ctatgacagt ctgcaggaag cctatgatct
     2701 ctttccaaaa gccgaacggt tgggacgatt actgatctcc gatctttttg tcaaaaagga
     2761 taaccggtat ttcgaccagt tgcgcaagaa cacgcgcgaa cgctttctcg attatgtccg
     2821 ctcgcatccg cagatgctgc agcgggt
//
LOCUS       DATGFG010000543         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1145207, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000543 DATGFG010000000
VERSION     DATGFG010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145207"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1713
                     /locus_tag="VKQ52_10160"
     CDS             67..1713
                     /locus_tag="VKQ52_10160"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLZ87596.1"
                     /translation="MSSRFPKLLFIGGLLLVLCCLHGYSQVGDSLAKKIDATFSEWDR
                     TNSPGVALAILKDGKIIYKRGYGMSNLEYGIAISPASIFHVASISKQFTAAAIQRLAL
                     EGKLSLDDDVRKYIPDMPNFGHTITIRNLVHHTSGLRDQWDLQWLAGWREGDLITEED
                     VLDMMKRQQALNFVPGDEYLYCNTGFTLLGVIVKKVTGVSLRNYTDSVFFKPLGMSST
                     HFHSDHTEIVPNRTSAYDRDGERWAIDIPVFDTYGATSLFTTVEDLAKWDENFYTARV
                     GGKAFIDSLLQPGVFNNGRPQNYASGLVIGEYKGRATVEHSGSDAGYRANFLRFPDQH
                     FSVIILANLGNISPSDLCHKVADIFLPPRPGLITRVTIVDSAMVSRWAGDYLEATSKA
                     RLKMEPLISPSMNSGTLMLNGVALTPRNDSVFVHPNNGVEYRFYSMGARVRLILRAPG
                     MQDRLYEKVKKITVSLAGLEEYTGAFYSKEVDARYTLFMKDYTLQVKMPRNEAMELTQ
                     YTKDLFTGPFTVEFLRDKKGKVSGFLLTTPRSRNIRFEKM"
     gene            1892..2155
                     /locus_tag="VKQ52_10165"
     CDS             1892..2155
                     /locus_tag="VKQ52_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLZ87597.1"
                     /translation="MSKTEKLLQRLLSIPKDFTWEELVKLLAYFGFEELKKGKTGGSR
                     RKFADERKRVIILHKPHPGNIVKEYAIRQVIEHLKEKGYIKNE"
BASE COUNT          583 a          501 c          659 g          566 t
ORIGIN      
        1 atcaggcctt tttatagatg aaacgtgaat gtagaatttt cctacctttc tgtaaaatgt
       61 tatcccatgt cctcccgttt cccaaaactc ctgtttatcg gcggtttgct actggtgctt
      121 tgctgtttgc atggctatag ccaggtgggc gattcgcttg ctaaaaagat cgacgccact
      181 tttagtgaat gggacaggac caactccccc ggcgtggcgc ttgcgatcct gaaggacggg
      241 aagatcatct acaaacgggg gtacgggatg tcgaatctcg agtacggtat cgccatctcg
      301 ccggcttcta tttttcatgt ggcctctatt tccaaacaat tcacggcggc agccattcag
      361 cggctggccc tcgaggggaa attgtcgctg gacgatgatg tccggaagta tatccccgat
      421 atgcccaatt tcggccacac gatcaccatc cgaaacctgg tccatcatac cagcggattg
      481 cgcgatcagt gggacctgca atggctcgcg gggtggcgcg agggcgacct gatcaccgaa
      541 gaggatgtac tggacatgat gaagcggcag caggcactga attttgtgcc gggtgacgag
      601 tatctttatt gcaatacggg gtttacattg ctgggggtga tcgtgaaaaa ggtgacgggg
      661 gtctcgctgc gcaattatac cgactcggtg tttttcaaac ccctggggat gagttctacc
      721 cattttcaca gcgatcatac ggagatcgtg cccaaccgga cctcggctta tgacagggat
      781 ggagaacgat gggcgatcga tatacctgta ttcgatacct atggcgccac gagtttgttt
      841 accacggtgg aggacctggc caaatgggac gagaattttt atacggcccg ggtcggcggc
      901 aaggcgttta tcgattccct gcttcagccg ggtgttttta ataacggcag accacaaaat
      961 tatgcatcgg ggctggtgat cggggaatac aaaggcaggg cgacggtgga acattcgggg
     1021 tcggatgccg ggtaccgggc taattttctg cgattcccgg accagcattt ttcggtgatc
     1081 atactggcca acctgggcaa tatcagtccg tcagacctgt gccacaaggt ggcggatatt
     1141 tttctgccgc cgaggccggg actgatcacg cgggtaacga tcgtggattc cgccatggtc
     1201 tcccggtggg cgggagatta tctcgaagcg acaagcaagg cccggctaaa gatggagccc
     1261 cttatctccc cttcgatgaa cagcggcacc ctgatgctca acggtgtcgc gctgaccccc
     1321 aggaatgatt ccgtatttgt gcatcccaat aacggggtgg aataccggtt ttattcgatg
     1381 ggcgcgcggg tccggcttat cctgcgggcg ccggggatgc aggaccggct atatgagaaa
     1441 gtaaagaaga tcacggtgtc gctggcgggg ctggaggagt ataccggggc attttacagc
     1501 aaggaggtgg atgcgcggta tacgcttttt atgaaagact atacgttgca ggtgaagatg
     1561 ccgaggaatg aggcgatgga gttgacgcag tatacaaagg atctttttac cggtccgttc
     1621 acggtggagt tcctgcggga taaaaaggga aaggtgagtg ggtttttgct gacgacgccg
     1681 cggtcgcgga atatacggtt tgagaagatg taggtcaatg ggttatgagg gggatttgga
     1741 ggattcggaa taatgaatta aattgcaact gaaatatagt tgcagatagc atattcgtat
     1801 ttttatcgcg cctatgttcg aacaaagtaa acctaaaaat aaatatggcg atggaatcaa
     1861 gaaaattcac taaatctaat tgctttaagc gatgtcgaag acagagaaat tactgcagcg
     1921 attgctatcc atccccaagg acttcacctg ggaggagcta gtcaaattac tggcttattt
     1981 tggttttgaa gaactaaaga aaggcaaaac gggaggatca aggcgcaaat tcgcagacga
     2041 acgaaagcgg gttattatct tacacaaacc ccatcccggc aatattgtaa aagaatatgc
     2101 gatccggcaa gtgatcgaac atttaaaaga aaagggatac atcaaaaatg aatgatgttt
     2161 tggaatataa aggctactat gcatcggttc attttagttc cgaagacgag gtgttttatg
     2221 gcaagctcct gggtatcgac gatctggtga atttcgaggg ctcgtccgta aaagagttga
     2281 agaaggcttt tcatgaagcc gtcgatgat
//
LOCUS       DATGFG010000544         5094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_121285, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000544 DATGFG010000000
VERSION     DATGFG010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121285"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VKQ52_10170"
     CDS             <1..996
                     /locus_tag="VKQ52_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLZ87598.1"
                     /translation="MDALPIVERTPVPFASKVRSTYNGQDVGVMHACGHDAHVAILMS
                     TATILAGMKKELKGTVKFVFQPAEEGPPTGEEGGADLMIKEGVLDNPKVDVMFGLHMA
                     ADVESGRIKYRSEGEMAASDWFTIKVHGKGSHGSQPWSGIDPIVISAQIIEGLQTIVS
                     RQTELTKDAAVISVCVIQGGVRSNIIPEEVQMTGTVRTLDTAMQRDIHERMRRTVTRI
                     AESAGATAEISIDNKTLITYNDPTLVRKMLPTMQRTAGAANVQVMDAMTGSEDFSFFA
                     ARVPAFFFFLGGMKPGQDPHTTAPHHTADFYLDETGFKLGVKTFCNLVFDYADAH"
     gene            1057..1368
                     /locus_tag="VKQ52_10175"
     CDS             1057..1368
                     /locus_tag="VKQ52_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HLZ87599.1"
                     /translation="MAKPAVEESFPFGETTLVFKVKGKMFLLTGLDSPVLEFNVKCDP
                     EKAIEWREQYAAVQPGYHMNKKHWNTVTADGSIPNRILKQMIDDSYELVIRSLPKKDQ
                     L"
     gene            complement(1359..1934)
                     /gene="nadD"
                     /locus_tag="VKQ52_10180"
     CDS             complement(1359..1934)
                     /gene="nadD"
                     /locus_tag="VKQ52_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HLZ87600.1"
                     /translation="MKIGLYFGSFNPIHHGHLLIATYILQNTPLQQVWFVVSPQNPLK
                     PAASLLNEYHRLYLVQLAIEGEKDLKALDIEFKLPKPSYTVDTLAYLQEKYPTHEFSV
                     IMGSDSFQNLSRWKNADWLLRNYSLYVYKRPEHENLPVYPGARDVQVLNAPLLPISST
                     DIRKSIKEGKSIRYWVPEPVRLEIERNGYYS"
     gene            complement(1931..2158)
                     /locus_tag="VKQ52_10185"
     CDS             complement(1931..2158)
                     /locus_tag="VKQ52_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HLZ87601.1"
                     /translation="MKRLSELEPGTVARIHSFEKNDLFLKLMEMGCVPGELIRVEQVA
                     PLGDPISITVAGYNLSLRLDEAENIFMEEEK"
     gene            2171..2605
                     /gene="ybeY"
                     /locus_tag="VKQ52_10190"
     CDS             2171..2605
                     /gene="ybeY"
                     /locus_tag="VKQ52_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HLZ87602.1"
                     /translation="MNNPIIQFNFLEPISLADRTRLKRFIVSLFKKEKTPLKEIQYIF
                     CSDPYILEINRQFLHHDYYTDIITFDLSEENHPINAEIYISVDRVRENAGQFASSVKT
                     ELQRVILHGALHLCGYGDKTTSEEKEMRKMEEKYLALYERFT"
     gene            2713..4575
                     /gene="mnmG"
                     /locus_tag="VKQ52_10195"
     CDS             2713..4575
                     /gene="mnmG"
                     /locus_tag="VKQ52_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HLZ87603.1"
                     /translation="MFPQYDVIVVGAGHAGCEAAAAAANLGSKVLLVTMNMQTIAQMS
                     CNPAMGGIAKGQIVREIDALGGYSGIVTDKSMIQFRMLNRSKGPAMWSPRAQNDRMMF
                     AATWREMLEQTPNVDFYQDMVNSLVIKDGRVAGVITGLGHTILSNAVVVTSGTFLNGV
                     IHIGEKRFGGGRVAEKAATGITEQLVSLGFESDRLKTGTPPRIDGRSLDYSKMEEQSG
                     DEEIIGFSYLDIPKPSRQKSCWITYTNTTVHEILKTGFDRSPMYTGRIEGVGPRYCPS
                     IEDKINRFAERERHQLFIEPEGWNTVEIYVNGFSTSLPEEVQYAALRQVPGFENVRIF
                     RPGYAIEYDYFPPTQLRHSLETKGLDNLFFAGQINGTTGYEEAACQGLMAGINAHQKI
                     SGQEPLILKRSEAYIGVLIDDLISKGTEEPYRMFTSRAEFRTLLRQDNADLRLTEMSY
                     RLGLASQERMEKTLEKKNGVEKVKEVLQTFSLEPAESADYLLEKNTAPLTQKQKAGQV
                     LLRPSIDLNSMTRAIPRLHEELKTYSPEILEQTEIQVKYDVYIEKEKDLVQKMANLED
                     LLIPENFNYDRISSLSNEALQKFKKIKPRTLGQASRISGVNPSDVQILMVFMGR"
     gene            complement(4638..>5094)
                     /gene="nadA"
                     /locus_tag="VKQ52_10200"
     CDS             complement(4638..>5094)
                     /gene="nadA"
                     /locus_tag="VKQ52_10200"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HLZ87604.1"
                     /translation="RKMILWPGACMVHEIFSLEKITKLKIRHPNAKLIAHPECEDPIL
                     RIADFIGSTTALLNYTKKDASMEYIVATETGILHQMQLSSPQKAFIPAPPTNNCACND
                     CPHMKLNTLEKLYLCMKYEEPELVMDPVLMVAARKPIERMLEISAKYGL"
BASE COUNT         1366 a         1398 c         1202 g         1128 t
ORIGIN      
        1 atggacgccc tgccgatcgt agagcggact cccgttccct ttgcaagtaa agtacggtcc
       61 acctacaatg gccaggatgt cggcgtcatg cacgcctgtg gtcacgatgc ccacgtcgcg
      121 atcctgatga gtaccgcaac catactcgcg gggatgaaaa aggagctgaa aggcaccgtc
      181 aaattcgtct tccagcccgc cgaggaaggc cctcccaccg gtgaggaagg cggcgccgac
      241 ctgatgataa aagaaggcgt cctcgacaac ccgaaagtgg atgtgatgtt tggcctgcac
      301 atggccgccg acgtagagtc gggcaggata aaataccgta gcgaaggaga gatggccgcc
      361 tcggattggt tcacgataaa agtccacgga aaaggctccc atgggtcaca accatggagc
      421 gggatcgacc cgatcgttat ctcggcccag atcatcgaag gactgcagac tatcgtgagc
      481 cgccagaccg aactgacaaa agatgccgcc gtcatttccg tctgcgtgat ccagggtggg
      541 gtccgttcca atatcatccc cgaagaagtc cagatgaccg gaaccgtcag gaccctcgac
      601 accgccatgc aacgggatat ccacgaacgg atgcgccgta ccgtcaccag gatcgccgaa
      661 agcgccgggg ccaccgcgga gatcagcatc gataataaga ccctgatcac ctacaatgac
      721 cccaccctgg taagaaaaat gcttcctaca atgcaacgga ccgcgggcgc cgccaatgta
      781 caggtgatgg atgcgatgac cggctccgag gatttttcct ttttcgccgc ccgggttccc
      841 gccttctttt tcttcctggg tgggatgaaa cccggccagg acccgcatac gactgccccg
      901 caccacaccg cggacttcta cctggatgaa acgggattca aattaggcgt gaagaccttt
      961 tgcaatcttg tattcgatta tgcagatgcc cattaagtat ttttgaattg ttttatacat
     1021 aacgagatgg atattgaaac actaagagag tactgcttgg caaaacccgc cgtcgaggaa
     1081 tcctttcctt tcggcgagac cacgctcgtc tttaaggtaa aaggaaagat gttcctgctc
     1141 accggcctgg atagccccgt cctggagttc aatgtcaaat gcgatccgga aaaagccatc
     1201 gaatggcggg aacaatatgc cgccgtacaa cccgggtatc acatgaataa aaagcattgg
     1261 aataccgtca ctgccgacgg tagcattccc aatcgcatcc tgaagcaaat gatcgacgat
     1321 tcatatgagc tggtaattcg ctccctgcca aaaaaagatc agctgtaata gccgtttctt
     1381 tctatctcca gccgcacagg ctccggtacc caataccgga tcgatttccc ctcttttatg
     1441 ctcttacgta tatctgtcga ggagatcggc aggagggggg cgttcaatac ctggacatcc
     1501 cgggcgcccg ggtagaccgg aaggttctca tgctccggtc gcttataaac ataaagggag
     1561 taattccgca acaaccaatc cgcattcttc catcgcgaca ggttttgaaa gctatcgctt
     1621 cccatgatga ccgagaactc atgagtggga tatttctcct ggagataggc aagggtatct
     1681 accgtgtagg aaggctttgg taatttgaac tcgatatcga gggccttgag atccttctct
     1741 ccttcgatgg ccaactgtac caggtagagc cggtgatatt cattgagcag ggacgctgcc
     1801 ggctttaaag ggttctgtgg ggagaccacg aaccatactt gttgaagggg agtattttgc
     1861 aggatatagg tggcaatgag taggtggccg tggtggatcg ggttaaagga tccaaagtag
     1921 agcccgatct tcatttctcc tcctccataa agatattctc tgcctcatcg agccgcaggc
     1981 tcaaattata tcccgccacg gtgatagaaa tcggatctcc caggggagct acttgttcta
     2041 ctctgatcaa ttcacccggt acacaaccca tttccattaa ctttaaaaag aggtcatttt
     2101 tttcaaatga atgaatcctt gcaacggtcc cgggttctaa ttctgataat ctcttcatct
     2161 ttgcaaaatt atgaataatc ccatcattca atttaatttc ctcgaaccca tctcactggc
     2221 cgatagaacg cggttaaaaa gatttattgt ctctcttttc aaaaaggaaa agaccccgct
     2281 gaaggagatt cagtacatct tttgttccga cccctatatt ctcgagatca accggcaatt
     2341 ccttcatcac gactattata cggacattat caccttcgat ctctccgaag agaaccaccc
     2401 aataaacgcc gaaatctata ttagtgttga tagggtaaga gagaatgcgg gacaatttgc
     2461 ttcttccgta aaaaccgaac tccaacgggt catccttcat ggagcccttc atttatgcgg
     2521 gtatggtgat aagacgacca gcgaagaaaa ggaaatgcgg aaaatggagg aaaaatacct
     2581 ggctctctat gaacgtttca cgtgaaacaa gcaaggtgca aaataattcc atttcccaac
     2641 cgttccacgt ggaacggcac caaacccaaa atattccgcc gccattaata aaggtcatag
     2701 taattttgca tcatgtttcc tcaatatgat gtgattgttg tgggtgccgg tcatgccggc
     2761 tgcgaagccg ctgccgccgc cgccaacctg ggatcaaaag ttctcctcgt aaccatgaac
     2821 atgcagacca tcgcccagat gagctgcaac cccgcaatgg ggggtatcgc caagggccag
     2881 atcgtccgtg agatcgacgc cctgggtggc tattccggca tcgttaccga taaaagcatg
     2941 atccagtttc gtatgctcaa tcggagtaag ggtcctgcca tgtggagccc ccgcgcccag
     3001 aacgatcgga tgatgtttgc cgcaacctgg cgggaaatgc tcgaacaaac ccccaatgtc
     3061 gatttctacc aggatatggt caattccctg gtgataaaag acggtagggt ggcaggcgta
     3121 ataacaggtc ttggtcatac catcctctcc aacgccgtgg tcgtcaccag cggaacgttc
     3181 ctgaacggag tcatccatat cggcgaaaaa cgctttggcg ggggtcgtgt cgcagagaaa
     3241 gcagctaccg gcatcaccga acaactcgta tccctgggat ttgaaagtga tcgcctaaag
     3301 acaggcaccc ctccccggat cgacggtcgc agccttgatt attcaaagat ggaggagcaa
     3361 agcggggatg aagaaataat tggtttctcc tatctcgata ttccaaaacc cagcaggcaa
     3421 aaaagctgct ggatcaccta tacgaataca accgttcatg agatcctgaa gactgggttt
     3481 gacaggagcc ctatgtacac cggccgcatc gaaggggtag ggccccgcta ctgcccaagc
     3541 atagaagata agatcaatcg cttcgccgaa cgcgaacgcc atcagctctt catcgaacct
     3601 gaaggctgga acaccgtcga gatctatgtg aacggtttct ccacctccct ccccgaagag
     3661 gtacaatatg cagccctccg ccaggttcca ggcttcgaaa acgtccgcat cttccgcccg
     3721 ggctatgcca tcgaatacga ctattttccg cccacccaac tccgtcattc cctagagaca
     3781 aaaggactcg acaatctctt tttcgcaggc cagataaacg ggaccaccgg ctatgaagaa
     3841 gcagcctgcc agggactcat ggccggcatc aacgctcatc aaaagataag cgggcaagag
     3901 ccgcttatcc tcaaacgcag cgaagcctat atcggcgtcc ttatcgatga cctcatcagc
     3961 aagggaaccg aagaacccta tcgcatgttt acctcccgcg ccgaattccg caccctgctt
     4021 cgccaggaca acgccgatct ccgcctcacc gaaatgagct atcgactagg actcgccagc
     4081 caggaaagaa tggagaaaac actcgagaaa aagaatggcg tcgaaaaagt aaaggaagtc
     4141 ctccagacct tctccctgga accggcagag tccgcggact atctcctcga aaagaacacc
     4201 gcccccctaa cccaaaaaca aaaagccgga caggtccttc tccgtccttc catcgacctc
     4261 aactcgatga ccagggccat cccccgtctt catgaagaat taaaaaccta ctctcccgaa
     4321 atactcgaac aaaccgagat ccaggtgaaa tacgatgtct atatagagaa agaaaaagac
     4381 ctcgtccaga aaatggccaa cctcgaagac ctcctcattc ccgagaactt caactacgac
     4441 cgaatctcct ccctgtccaa cgaagccctg cagaaattca aaaagataaa accaaggacc
     4501 ctcggccagg caagccgtat cagcggcgtc aacccgagcg acgtacaaat cctcatggtc
     4561 ttcatgggga gataagataa aaaaaggaca gaaaaagcat tctgtccttt taatttattg
     4621 ataatcaatt atttacgtca taatccatac tttgcgctga tttccagcat ccgttctatc
     4681 ggctttctcg ccgcaaccat cagcacggga tccatcacca gctcgggttc ttcatacttc
     4741 atacacaggt atagcttctc aagcgtattt agcttcatat gcgggcagtc attgcaggca
     4801 cagttgttgg tcggcggagc agggataaag gccttctgtg gtgaggataa ctgcatctgg
     4861 tgcaatatcc ccgtctcagt cgccacgata tattccattg acgcatcttt cttcgtataa
     4921 ttcagcaacg ccgtcgtcga accgataaaa tccgcgatcc gtaaaatggg gtcctcacat
     4981 tcggggtgcg cgatcagttt tgcattcggg tgacggatct tcagctttgt gatcttctcc
     5041 aggctgaaga tctcatgcac catgcaagcc cccggccaga gaatcatctt gcgg
//
LOCUS       DATGFG010000545        13259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_575774, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000545 DATGFG010000000
VERSION     DATGFG010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13259
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575774"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3560)
                     /locus_tag="VKQ52_10205"
     CDS             complement(<1..3560)
                     /locus_tag="VKQ52_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87605.1"
                     /translation="MSAQPSEHPPRRSWPRRLVRIFVRTLLTLLVILLLVFFLIQTPF
                     VQNIARGKVETYLSRKLRVPVRIGHLDISFFRSVTLRDIYIEDRHKDTLLSAGLVSVR
                     LRMLALLHNNLSIQEIHLQNVTANILRRSPNDTTFNYQFLIDAFVGKPAAKPDTAKST
                     PLQISMNTLLLDNIHFVYADTLTGNTAIITIGHNKTDMRALDLDKQIYQVSGFSLANT
                     SLFYRNSKTAMATSARVTGLHAGDLLLNLDDLTIRSRSLELDSTNFAFDNDKQPRRKK
                     GMDYYHLLANDITLHGKDLVYSASGISGLITKGELAERSGFRLNQLQTRFFYSDHKAV
                     LEDLLLRTPGTLLRRSATLQYDSITAITTNPAHTLLSLDLPNSHVQLKDILVFAPFLG
                     SQPVFSHPNDTWQINTAVSGNLDALTVQTLQFSGIKDLKLDMAGKLLHPMNSRRIRAD
                     LDIRNVSGSRTALLALLPKNTLPGNITIPARYSLHGHLAGSMDDMISDLTLNTTSGSI
                     LLKGTARNFRSTTAVTYDLTIQTKALDLGAILQDTTQWGAVTSGFHIKGTGLDIHNAN
                     VAFNGAITAATFRQYTWHDLAIDGSLANQQAVLHSAINNTAVHFELQATADLARKFPA
                     LNLDWNIDTLDLHAIHLTKDTLQFKGHLTAGFADTNPDSLQGTLKLTNIDLLRGTQRL
                     STDSIILVATRQEDIEEIRLHSEMADLDWHGRYKLTETATALEHTIDKYYHLSGFKDT
                     AFTPQDWTMELNLRASPMALVLMPALRGTDTIGARIAFNSDRNDLHLGIRTPRIVYGT
                     AFFHDIDLSAGTADSSLRYNLTLADGHGSGILLYKTSLYGALQDDHLATALLLRDGKG
                     KDRYRIAGTLDQQQGQGPIKFRFAPDSLLLNYDRWQVSRDNFIQYDSTGLIAHDLTIS
                     NNNDSLSLNSKGTTGTAPLDVRFGNFRLSTISRLANQDSVIADGILVGNAEVKNVTTN
                     PVFTSDLKIQNLSYKSDTLGDLTLKVNNEKANAFSADIGLEGKNNDIAITGDYYTGDS
                     RMDLKLDLRRINLSAFSHSIEGVVDNMKGYLMGKFTIAGTVDKPNVRGNLYFDSSVIT
                     PTISGEALDVSKDRIAFDEDGFNFSQFELRDSAGNKLVIDGNVYTKDYRSFGFDISLN
                     AQNFRMVNAPENSSRQFYGQLNLDAAINLEGKLDAPKVDG"
     gene            3801..5225
                     /gene="rpoN"
                     /locus_tag="VKQ52_10210"
     CDS             3801..5225
                     /gene="rpoN"
                     /locus_tag="VKQ52_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HLZ87606.1"
                     /translation="MLKQVQTQKQQHTILPQQIELLNLFHLNTLELEQRIQDELSENP
                     ALEELGAGDEASSDKFSKDTIQDFQGWEEYAYDDIPDYKTEYNNYINTEKVPEKPIAE
                     VPDFREELKKQCRFLDVPEDKHYLFDFLIDSLNENGFLEQDLEELASEISFKKKVWIE
                     PEELEGVLKLLRELEPFGAGCRSVQEYMLLQLRRMNVKRPDVRTAICLLQEHFHDLRS
                     CNLEKLRRQLGLDDDEIRIVLNLLAHLGTRPFNPENNAMQANPSILPDFIVIDEGDTL
                     ELALYRQRSSNLHISQSWMEMVQQASQNTNTEKSTKQYLRSKLSSAQWFINAIQQREN
                     NMLKIMRAILDMQYEYFKYGDILMLRPMKLKDIADRVKVDISTVSRLTCNKYAETPFG
                     TVLLKDLFTEGIVNQEGNNISNRVIQNIIGEVIDAEDKKAPYTDRQLVTILAEKGYSI
                     ARRTVAKYRELMNIPVAQVRGLWN"
     gene            complement(5269..5943)
                     /locus_tag="VKQ52_10215"
     CDS             complement(5269..5943)
                     /locus_tag="VKQ52_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HLZ87607.1"
                     /translation="MKNNNLFVLDLRVFLLGTALLLATTLLGQQQQTSTESNLKAKFG
                     IKGGFDLTTLYSSDVSTNHMKPGFDAGVFAKLPVTRGFSIQPELLYSLKGAKNTYSNF
                     IEGSGEYRFNLGYLQLPVLAVVNLAPNFNLHLGGYAAYLVNANIKNVDNNGNIQSATE
                     LNTGDFNRWDFGLVGGLGFDIENVTLGARYNYGLSEIGKSGNATGDLTKNQKNAGFSI
                     YVGFGF"
     gene            6028..6798
                     /locus_tag="VKQ52_10220"
     CDS             6028..6798
                     /locus_tag="VKQ52_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HLZ87608.1"
                     /translation="MRSIWTGAIGFGLVNIPVKLYSATQGSELDLDMLDKKDHANIKF
                     KRVNANTGKEVAWENIVKGYKLDDRYVVLTDEDFQKASPEKTKTIAIEEFIKETEIDG
                     IYYETPYYLEPDKSGARAYVLLRDALHKTGMAGFGSFVLRNKEGLCLIKPMEDILVLN
                     RIRWAQEIRPTDEIKVPGGAPKPAEMKMALELIRQLAGPFDIAKYKDSYSDELMKLIK
                     AKAKGKAPKPALRVVHKTKSTDLLSQLKASLGGKKKAS"
     gene            complement(6827..7759)
                     /locus_tag="VKQ52_10225"
     CDS             complement(6827..7759)
                     /locus_tag="VKQ52_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLZ87609.1"
                     /translation="MKKPFRLSAVWKAMKRAAIDFNNDNGFKLAASLSYSMIFSIGPV
                     LIVVISLAGIFWGQQAVEGRIYQQIKDLVGASAAVQIQDIIRNIQRSHHSVAGAIIGG
                     VILVIGATGVFAEIQGSINYMWSILAKPKKGWLKLLLNRLLSFSLILAFGFISMVALI
                     INSVMDLLGDYLKKYFSHFTVYFFYSVNLLLSIGAIILLFMIIFRILPDAILRWKDAF
                     IGAVFTALLFLLGKFLIGLYLGNSNIGILYGAAASFIVILTWVYYSSIILFYGAEFTK
                     AYANIAGYGVRPSESAVFIIKSESREIDGELGSD"
     gene            8142..9278
                     /locus_tag="VKQ52_10230"
     CDS             8142..9278
                     /locus_tag="VKQ52_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLZ87610.1"
                     /translation="MKELKFASPLRTASILIILVICFVILSWAADVIVPLLFAIIFSS
                     MLFPICYQLEKWGCHKGVAAFISVLLAGAVTALFLGVVLMQVMHLVEQGPQFVARLTS
                     LLDRVETVVAQRFHIEKSTQADHVHLLLNKALDNSSAFLSSTMSFTTNILSEFLLILL
                     FGFFLLYLRVFFLEFFYKVFPSSDKLLIDDTLQKIYAVIQNYLLGLLKVICIIGTLNS
                     IGLWALGIESPLFFGFLGGMLVVIPYIGILIGAALPVIVALVTKDSYWYAGGVLGVFL
                     FVHILEGNVITPYVVGSKVSINPLVAVFSLLLFGKLWGLSGLILALPVTAICKIIFDT
                     LPGFKAVGFLLGKPQKYHFKRYSILHAKLRRYNKAAPVNRGGVH"
     gene            9596..10708
                     /locus_tag="VKQ52_10235"
     CDS             9596..10708
                     /locus_tag="VKQ52_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HLZ87611.1"
                     /translation="MPDQFLNQFDHFPALIWDAILVAVALVIGLVLKFLLSLLLRRKP
                     REGRPLFSVTRSMLARLGQPFSYFLPLLTLNFLLPLMKMKATVMPFANKTIEILLTIS
                     FANVLIAVVKVFEDYAYHTFDIQKADNLKERKIRTQLQFIRRLAISLILILTVCFILL
                     SFNNLRKIGTGLLTGVGVGGIIIGFAAQRSLGNLLAGFQIAFTQPLRIDDVLVVEGEW
                     GRVEEITLTYVVLNIWDQRRLILPINYFVEKPFQNWTRTTADILGTVFLYLDYSVSLD
                     GLRAELGRLLENDPLWDKRVKVMQVTNTSEKTMEVRALMSATSSGNAFDLRCNVREGL
                     ITYLQKSYPDAFPVDRVVLKRASMPATSKLREDYAS"
     gene            complement(10696..11151)
                     /locus_tag="VKQ52_10240"
     CDS             complement(10696..11151)
                     /locus_tag="VKQ52_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87612.1"
                     /translation="MAKKDKNTIDLPEVKDIPGQENIKPPRLGELADTTASSADEEGD
                     DVLEQDDDLAGPGSNVSRDERRALANVDNREVDTEDEVLNEAALDSTDDDGDPLNEGS
                     FGSDVSSDDLDIPGADEDDSDEELGEEDEENNDYSISDNNDDTDINYDA"
     gene            complement(11527..12333)
                     /locus_tag="VKQ52_10245"
     CDS             complement(11527..12333)
                     /locus_tag="VKQ52_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HLZ87613.1"
                     /translation="MRTLITLLLLSLCVADAQAQYKLVWSDEFNYTGLPDSTKWSYDV
                     GGNGWGNNELEYYSNADTNNAVVRNGHLYITARKQQISTATATTRIVRPYTSARLVTK
                     FKGDWLYGRIEVRAILPAGRGLWPAIWMLPTDWKYGGWPASGEIDIMENVGFMPDSIF
                     GSIHTKSFNHVIHTQKTKGLFIKDPYTAFHTYAVEWDTQQIRFFVDGNVYTSFHNTGK
                     GFAEWPFDQRFHLLLNIAVGGGWGGQKGIDDNSFPSSMIVDYVRVYQSAK"
     gene            complement(12330..>13259)
                     /locus_tag="VKQ52_10250"
     CDS             complement(12330..>13259)
                     /locus_tag="VKQ52_10250"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HLZ87614.1"
                     /translation="QLKQEGFEIPILPAFRPDNAMNVDDPAKFNGYIKRLESVTNVAI
                     SNFNDYLFALENRHDFFASMGCTVSDHGLEEIYVEEFTGWEVEAIFNKVRAGKELNDT
                     ERRKFKSAMLVHFAEWDWEKGWVQQFHLGALRNNNSRMTQKLGPDTGWDSIGDFSQGR
                     ALGRFLDRLDKEDKLARTILYNLNPADNELFATMIGNFNDGSIAGKVQYGSGWWFLDQ
                     KDGMTRQINALSNMGLLSRFVGMLTDSRSFLSFPRHEYFRRILCNLFGEEITNGELPN
                     DLEWIGKIVRDICYYNARQYFNFQPTGALAYSE"
BASE COUNT         2754 a         2892 c         4250 g         3363 t
ORIGIN      
        1 tcgccgtcca cctttggcgc atccaatttc ccttcgaggt tgatggccgc gtcgaggttg
       61 agctggccat agaactggcg ggagctgttc tcgggggcgt tgaccatgcg gaagttctgt
      121 gcgttcaggg aaatgtcgaa tccgaagctg cgataatcct ttgtatagac gttgccgtcg
      181 atcaccagct tgttgccggc cgagtcccgc aattcgaact ggctgaagtt gaaaccgtct
      241 tcatcgaagg cgatgcgatc tttcgagacg tccagggctt cgccggagat ggtcggagtg
      301 atcacgctgc tgtcgaagta aagattgccc ctgacattgg gtttatcgac agttccggcg
      361 atggtgaatt tccccatgag gtagcccttc atattgtcga cgactccttc tatggagtgt
      421 gaaaaggccg agagattgat gcgtcgaagg tcgagcttca ggtccatcct gctatcaccc
      481 gtgtaatagt caccggtgat agcgatatca ttgttcttgc cttcgaggcc gatgtcggcg
      541 gagaaggcgt ttgccttttc attgttgacc ttcagggtca ggtcaccgag ggtatcgctc
      601 ttataggaca ggttttgaat tttcagatcg gaggtaaaga ccgggttggt ggtcacattc
      661 tttacttcgg cgttgccgac cagaatgcca tctgcgatga ccgagtcctg gttggccagg
      721 cggctgatgg tgctgagccg gaaattgccg aaacggacgt cgaggggggc tgtgccggtg
      781 gtccctttgc tgttgaggga aagggaatcg ttgttgttgc tgatggtaag gtcgtgggct
      841 atgaggccgg tggagtcgta ttgaatgaag ttgtcgcggc tgacctgcca gcggtcgtag
      901 ttgaggagga ggctgtcggg ggcgaacctg aattttatcg ggccctggcc ctgctgctgg
      961 tcaagtgtcc cggcgatgcg atagcggtcc tttccttttc cgtcacgcag gaggagggca
     1021 gtcgccaggt gatcgtcctg caatgcgccg taaagggatg ttttatacag caggatgccg
     1081 ctgccatgac cgtcggccag ggtgaggtta tagcgcagac tgctgtcggc tgttccggcg
     1141 ctgagatcga tgtcatgaaa gaatgctgtc ccgtagacga tgcgcggcgt tcttatcccc
     1201 agatgaagat cgtttcgatc gctgttaaag gctatgcggg cgccgatggt atcggttcct
     1261 cttaacgcag gcatgaggac cagcgccatg ggcgaggccc ggagattgag ttccatggtc
     1321 cagtcctgcg gggtaaaggc ggtgtctttg aaacccgaga gatgataata tttatcgatc
     1381 gtatgttcga gggcggttgc tgtttcggtg agtttatacc ggccgtgcca gtcgaggtcg
     1441 gccatttccg agtgaagccg gatctcttcg atgtcctctt gccgggttgc cacgaggatg
     1501 atgctgtcgg tcgagaggcg ttgggtaccc cgaaggaggt cgatgttggt gagttttagc
     1561 gtaccctgga gggagtcggg gttggtatcg gcaaagccgg ctgtgagatg tcctttgaac
     1621 tgaagggtgt cctttgtcag gtggatggcg tgaagatcga gggtatcgat attccagtcg
     1681 aggttgaggg ccgggaattt ccgggccaga tcggcggttg cctgtaattc gaagtgtacg
     1741 gcagtgttgt ttatggcgga atggaggacg gcctgctggt tggcgaggct gccgtcgatg
     1801 gcgaggtcgt gccaggtgta ttgccggaag gtagcggccg tgatggcgcc gttgaaggcg
     1861 acgttggcgt tatggatgtc gaggcctgtg ccttttatgt ggaagccgga ggtgacggcg
     1921 ccccattggg tggtatcctg caggatagcc ccgagatcca gggcttttgt ttgtatcgta
     1981 agatcgtagg tgacggccgt cgtcgaccgg aagttgcggg ccgttccctt gagaaggata
     2041 ctgcccgatg tggtattcag ggtcagatcg gagatcatgt cgtccatgct gcccgcgagg
     2101 tgtccatgga ggctgtagcg agcggggatg gtgatattgc cgggcagggt attctttggc
     2161 aacagggcca ggagggctgt acggctgccc gatacgttac ggatatcgag gtcggcgcgg
     2221 atgcggcggc tgttcatggg atggaggagc ttaccggcca tgtcgagttt caggtccttt
     2281 atcccgctga actggagggt ttggaccgtc agcgcgtcga ggttaccgct gacagccgtg
     2341 tttatctgcc aggtgtcgtt tggatggctg aataccggtt gactacccag gaagggggcg
     2401 aagacgagga tatcttttag ttgaacgtga ctgttgggga ggtcaaggct gagtaaggtg
     2461 tgggcggggt tggtcgtgat ggccgtgatg gaatcgtatt gaagggttgc ggatcgccgc
     2521 aggagggtgc cgggggttct caggaggagg tcctctagca cggctttatg atcggagtag
     2581 aagaagcggg tctgtagctg gttgaggcgg aagccggacc gttcggccag ttctcccttt
     2641 gtgataagac cgctgatgcc ggaggcgctg tagaccaggt ctttgccgtg tagggtgatg
     2701 tcgtttgcca gcaggtgata gtaatccatg ccttttttcc ggcggggttg tttgtcgtta
     2761 tcgaaggcga agttggtgct gtcgagttcg agggaccggg aacggatggt aaggtcatcc
     2821 aggttcagga gcagatcgcc ggcatggagc ccggtgacgc gggcgctggt agccatggcg
     2881 gttttggagt tgcggtagaa gaggctggtg ttggcgaggc tgaagccgga cacctggtag
     2941 atctgcttat cgaggtcgag ggcccgcatg tcggtcttgt tgtggccgat ggtgatgatg
     3001 gcggtgttgc cggtgagggt atcggcgtat acgaaatgga tattatcgag gaggagggtg
     3061 ttcatgctga tctgcagggg agtgcttttc gccgtgtctg gttttgcagc gggcttacca
     3121 acgaaggcgt cgatgaggaa ctggtaattg aaggtcgtgt cgttgggtga ccgacggagg
     3181 atgttggccg tcacgttctg caggtggatc tcctggatcg acaggttgtt atgaaggagg
     3241 gccagcatcc ggagccggac gcttaccagg ccggcggaga ggagggtatc cttgtggcgg
     3301 tcttcgatat agatgtccct gagggtcacc gaacgaaaga agctgatgtc gaggtggccg
     3361 atgcgaacgg gaaccctgag ctttcgggaa agataggtct cgacctttcc gcgggcgatg
     3421 ttctggacaa agggggtttg gatgaggaag aagacgagaa gcaggatgac gagcagggtc
     3481 aggagggtcc tgacgaagat gcgcaccaac ctacggggcc aggatctgcg aggcggatgt
     3541 tcggatggtt gtgcgctcat aaattatgat tctgaataat ataagttagg ttaatctggt
     3601 aacccgacaa aatttagtcc atcttgtgca aatgacggga gaacatattg tcattcaatg
     3661 aatttgttcc gttttttccc caaaaccgtc ggtttttttc tacaggtggg cggtggcatg
     3721 ggtgctttcc cgttttagga caagcggtta agattctggc aactcgtttg tcctatccat
     3781 tatcactaaa accgggtacc atgttgaagc aagtacagac gcaaaaacag caacacacta
     3841 tcctgccgca acagatcgag ttactgaacc tatttcatct gaataccctg gagctggaac
     3901 agcgaataca agatgagcta agcgagaacc cggccctgga ggagctgggg gcgggtgatg
     3961 aagcgtcatc ggataagttt tccaaggata cgatccagga ttttcagggc tgggaagagt
     4021 atgcgtatga cgatatcccg gattacaaga cggagtacaa caattatatc aataccgaga
     4081 aggtgccgga gaagccgatc gccgaggttc cggatttcag ggaggagttg aagaagcaat
     4141 gccgtttcct ggatgtgccg gaggacaagc actatctttt tgattttctt atcgattcgc
     4201 tgaatgagaa tggtttcctg gaacaggacc tggaggagct ggcctcggag atctctttca
     4261 aaaagaaggt gtggatcgag ccggaggaac tggaaggcgt gctgaagctg ttgcgcgaac
     4321 tggagccgtt tggggcgggt tgccggtcgg tgcaggagta tatgctgctt caattgcggc
     4381 ggatgaatgt caagcgcccg gatgtgcgta cggcaatttg tcttctccag gagcattttc
     4441 acgatctgcg aagctgcaac ctcgaaaaat tgcggcggca gttggggctc gacgatgatg
     4501 agatcaggat cgtgctgaac ctgctggcgc atctcggtac ccggcctttt aatcccgaga
     4561 acaatgcgat gcaggccaat cccagcatat tgccggattt tatcgtgatc gacgaagggg
     4621 atacgcttga actggcgtta taccggcagc ggagcagcaa tttgcatatc agccagtcgt
     4681 ggatggagat ggtacagcag gcctctcaga atacgaatac cgaaaaatcg accaagcaat
     4741 acctgcgcag caagctttct tctgcgcagt ggtttatcaa tgcgatacag cagcgggaga
     4801 acaatatgct gaagatcatg cgggcgatac tggatatgca gtatgaatac ttcaaatatg
     4861 gagatatcct tatgttacgg ccgatgaagt taaaggatat tgccgaccgg gtgaaggtgg
     4921 atatttccac cgtgagccgg ctcacctgta acaagtatgc ggagacgccg tttggaacgg
     4981 tgttgttgaa ggatctgttc acggagggga tcgtcaacca ggagggtaat aatatcagca
     5041 atagggtgat ccagaacatc atcggggagg tgatcgatgc ggaggataag aaggcgccgt
     5101 atacggaccg tcaattggtg acgatccttg cggagaaggg gtattcgatt gcccggcgga
     5161 cggtagccaa atacagggag ctgatgaata tcccggtggc gcaggtgagg gggctttgga
     5221 attaaagatc ttgaaagagg ccccgcttgc acggggcctt ttctttgttt agaagccaaa
     5281 gccgacgtag atggagaacc cggcgttctt ttgattcttt gtcagatcgc cggtagcgtt
     5341 accggatttg ccgatctcgg acaggccgta attgtaccgg gcgccgaggg taacgttttc
     5401 gatgtcgaag cccaggccgc cgaccaggcc gaagtcccag cggttgaagt cgccggtgtt
     5461 cagttcggtg gcgctctgga tattgccgtt gttatctacg ttcttgatgt tggcgttcac
     5521 caggtaggct gcatagccgc cgagatgcag gttaaaattg ggagcgaggt tgacgacggc
     5581 cagtacgggc agctgcaggt agccgaggtt gaagcgatat tcgccggagc cttcgatgaa
     5641 attgctgtag gtgttctttg cgcccttcag gctgtagagc agttccggct ggatggagaa
     5701 gccccttgtg acgggcagtt tagcgaagac gccggcgtcg aagcctggtt tcatatgatt
     5761 ggtggatacg tcgctgctgt ataaagtagt cagatcgaat ccgcctttta tcccgaactt
     5821 tgctttgagg ttggactcgg tggaggtttg ttgttgctgg ccaaggaggg tagtggccag
     5881 cagtaaggct gtacccagca ggaagacgcg cagatccagt acgaataggt tgttgttttt
     5941 cataattaac gtttctgaac aaaaatcaat tgttatgccc ggcccggctg gcatgcaatt
     6001 tattccaaca ccttaaaaca catctatatg agatcgatct ggacgggggc aatcggcttt
     6061 ggcctggtga acatccccgt aaaactgtac agcgccacgc agggcagcga actggacctg
     6121 gacatgcttg acaagaagga tcatgcgaat atcaaattca agcgggtgaa tgcgaatacg
     6181 ggcaaggagg tagcctggga gaatatcgtc aaggggtata agttggacga ccggtatgtg
     6241 gtgctgacgg atgaggactt tcagaaagcg agtcccgaga agaccaagac gatcgccatc
     6301 gaggagttta tcaaggagac ggagatcgac ggcatttatt atgagacgcc ttattacctg
     6361 gagccggata agtcgggagc gcgggcgtat gtgctgttgc gtgatgcgct tcacaagacg
     6421 gggatggccg gtttcgggag ttttgtgtta cggaataaag aggggctttg cctgatcaag
     6481 ccgatggagg atattcttgt cctgaaccgg atccggtggg cgcaggagat ccggcccacg
     6541 gatgagatca aggtcccggg tggggcgccg aagccggcgg agatgaagat ggcgctggag
     6601 ttgatcaggc agttggcggg gccattcgat atcgcgaaat ataaagattc ctattccgat
     6661 gagctgatga aactgataaa ggcgaaggcg aagggtaagg cgccgaagcc ggcgttgcgg
     6721 gttgtgcata agacgaagag tacggatctg ttgtctcagt tgaaggcttc gctgggcggg
     6781 aagaagaagg ccagttagtt tgcgggcgag gatgccgggc cggcgatcag tcggagccca
     6841 gttcgccgtc tatttccctt gattcacttt tgatgatgaa gacggccgat tcggatgggc
     6901 ggacgccgta acccgctatg ttggcgtagg cctttgtaaa ttccgcgccg tagaagagga
     6961 tgatgctgga atagtagacc caggtgagga tgacgatgaa ggaggcggcg gcgccataga
     7021 ggatgccgat gttggaatta cccaggtaga ggccgatgag aaatttcccc agcaggaaga
     7081 gcagggcggt aaaaacggcc ccgatgaagg cgtccttcca gcggaggatc gcgtcgggca
     7141 ggatgcggaa gatgatcatg aacagcagaa tgatggcgcc gatgctgagg aggaggttga
     7201 ccgaataaaa gaagtagacg gtaaagtggg agaagtattt tttcagatag tcaccgagca
     7261 ggtccatgac ggagttgatg ataagtgcga ccatcgagat aaagccgaag gcgagaatga
     7321 gggaaaagga gagcaggcgg ttgaggagca gcttcagcca gccttttttg ggttttgcga
     7381 ggatggacca catataattg atggagccct ggatctccgc gaagacgccg gtggcgccga
     7441 tgacgaggat caccccgccg atgatggcgc ccgccacgga atggtggctg cgttgaatat
     7501 tcctgatgat atcctgtatc tgaaccgcgg cgctggcgcc gacgaggtct tttatctgct
     7561 ggtagatgcg gccttcgacg gcctgctgtc cccagaagat gccggccagg gagatcacga
     7621 cgatgagtac ggggccgatg gaaaagatca tggaatagga aagggaggcc gccagtttga
     7681 agccgttgtc gttattaaaa tcgatcgcgg cccgtttcat ggctttccag acagccgaaa
     7741 gtcgaaaggg tttcttcatt tttgtagagt ttattcccca gtttggtggt ttggaggcgt
     7801 tttttacggt caaagaaaat tggcaagcat ttttcactag tatgaataga taaaattatt
     7861 ccttactact aaaaatcatg ttatgagcgg cttactttat ttaatagcag tgatcttgat
     7921 cataggctgg ctgttggggg tatttgtata tagcgtaggt gggctgatcc atgtattaat
     7981 tgtattggca gttatatcgc tgatcctggg tatcatcagg agggcctgac cgctttgttt
     8041 cggggtaaag ggcttgttta caggcccgca aggccggtcg taagaccggc tcttttttta
     8101 gccgtcacca gcggcatatt catcatcaat cgtcgtcact catgaaagaa ctgaaatttg
     8161 cctcgccgct gcgtactgcg tccatcctga tcatcctggt catctgtttt gtcatcttat
     8221 cctgggcggc ggatgtgatc gtaccgttgc tttttgccat tatcttttcg agcatgttat
     8281 tccccatttg ttaccaattg gaaaaatggg gctgtcataa gggtgtcgcg gcctttatct
     8341 ccgttttgct ggcgggggcg gtcacggcgc tttttcttgg ggtggtgctc atgcaggtga
     8401 tgcaccttgt ggagcagggg ccgcagtttg tggccaggct gacgtcgttg ctcgaccggg
     8461 tggagacggt ggtggcgcag cgttttcata tcgagaagtc gacacaggcc gatcatgtgc
     8521 atcttttgct gaacaaggct ttggataaca gcagcgcctt tctttcttcg acgatgtcct
     8581 ttaccacgaa tatcctgtcc gagtttctgc tgatcctttt attcggtttc tttttgttgt
     8641 atctgcgggt atttttcctg gaattctttt acaaggtgtt tccttcttcg gacaagttgc
     8701 ttatcgatga cacgctgcag aagatctatg cggtgatcca gaattacctg ctcgggttgt
     8761 taaaagtgat ctgtatcatc ggaacattga attccatcgg gctttgggcg ttggggatcg
     8821 aatctccgtt gttctttggg ttcctggggg ggatgctggt ggtcattccc tatatcggca
     8881 tcctgatcgg ggctgcgttg cccgttatcg tggcgctggt gaccaaggat tcgtactggt
     8941 atgcgggcgg ggtgctcggg gtgttcctgt tcgtacatat cctggagggg aatgtgatca
     9001 cgccgtatgt ggtggggtcg aaggtgagca tcaatccgct ggtggcggtg ttctcgctgt
     9061 tgctttttgg gaagctgtgg gggctgtcgg ggttgatcct ggcgttgccg gtgacggcga
     9121 tctgtaagat catattcgac acgttgccgg ggttcaaggc ggtggggttt ctgctgggca
     9181 agccgcagaa gtatcatttc aagcgatatt cgatcctgca tgcgaagctg aggcgatata
     9241 ataaagccgc cccggtgaac cggggcggcg tccattaacc taaacctcta tcgcacaaaa
     9301 tagttgttga tcgtcgaagg cgtcgcggag ttcgtcggtg gggcggcctt gctcattttc
     9361 agctcctgca ggggggccgg catttccatg tcctcacttt agggtcttac cgaaaaagat
     9421 atgccaggga aattactgcg gtaaaactgc ggggaatggg gaaatgggga ggttttgggg
     9481 cgatcttttg gatggttttg gggcttttgg ggaatggccg gccgatcggg tcctttttgg
     9541 ggaagaattt ggctcaaacc ttgcaattta tccgtcagga tcaagaaatt aacctatgcc
     9601 cgaccagttt ttgaaccaat tcgaccactt ccccgccctg atctgggatg ccatcctggt
     9661 ggctgtggcg cttgtcatcg gtcttgttct caaattcctg ttatcgttgc tgttgcggag
     9721 aaagccaagg gagggccgcc cgcttttttc cgtgacccgg agcatgctgg cccggcttgg
     9781 tcagcctttt agctatttcc tgcccctgct gacgctcaat ttcctgttgc cgctgatgaa
     9841 gatgaaggca acggtaatgc cttttgcgaa taagacgatc gagatcctgc tgacgatctc
     9901 ttttgccaat gtgttgatcg ccgttgtaaa ggtgttcgag gattatgcct atcatacgtt
     9961 cgatatccag aaggcggaca acctgaaaga gcggaagatc aggacccagc tgcagtttat
    10021 ccggcggctg gcgatcagtc ttatcctcat ccttaccgtt tgttttattc tactgagttt
    10081 caacaatctg cggaagattg ggaccggctt gctgacgggt gtgggtgtgg gggggatcat
    10141 cattggtttt gcggcgcagc ggtcgcttgg gaacctgctg gccggtttcc agatcgcttt
    10201 tacccagcct ttgcggatcg acgatgtcct tgtggtggag ggggaatggg ggcgggtgga
    10261 ggagatcacg ctcacgtatg tggtgctcaa tatctgggat cagcggaggc tgatcctgcc
    10321 gataaattat tttgtcgaga agccgttcca gaactggacc cggaccaccg cggatatcct
    10381 gggcaccgtc tttctttacc tggactacag tgtttccctg gatgggcttc gggcggagct
    10441 ggggaggctg ctggagaatg atccgctttg ggataagcgg gtgaaggtga tgcaggtgac
    10501 gaatacgagt gaaaagacga tggaggtacg cgcgctgatg agcgcgacca gcagtggtaa
    10561 tgcgttcgat ctgcgttgta atgtgcggga ggggttgatc acctatctgc aaaagagcta
    10621 cccggatgcc ttcccggtag acagggtcgt attaaaaaga gcttcgatgc cggcgacatc
    10681 gaagctccgg gaagactatg cgtcgtaatt gatgtcggtg tcgtcgttgt tgtcgctgat
    10741 gctgtagtcg ttgttctctt cgtcttcttc gccgagttct tcgtcgctgt cgtcttcgtc
    10801 ggcaccgggg atatccaggt cgtcgctgga gacatcgctg ccaaagcttc cttcattcaa
    10861 cggatcgccg tcatcgtcgg tgctgtcgag ggctgcctcg ttgaggacct cgtcttcggt
    10921 gtcgacctca cggttatcga cattggccag ggctctgcgt tcatcgcggg atacgttgct
    10981 gccgggtcct gcgagatcgt catcctgttc caggacatca tcgccttctt cgtcggcgga
    11041 ggaagctgta gtatcagcca gctctcctaa acggggtggc ttgatatttt cctggcctgg
    11101 gatgtctttt acctcgggga ggtcgatcgt gttcttgtcc ttttttgcca taccgtttgt
    11161 tttagacttg taagttaggg atttttcggg ccgtcccggg gttaagaaat cggtccgggc
    11221 ctgggttggc tatgcaattc gggtgcccgg cgcatgcacc aggactgcag ggaagcaaaa
    11281 cagcattttc acagaatccg ttgctaatac gatttatcat ttataaatat gaaatattta
    11341 tattcgttag ttattaattt taaaaagagt aaggttttca tataaaaatc aatcatttct
    11401 ccataaataa taagggttat atttagaact tacttacttt agcagtagga taaggtctaa
    11461 attcgagtcg actcactacc cggtattttc atgccgggtt ttctttttta aggggagtat
    11521 atgggtctat tttgcggact gatagaccct gacgtaatcg acgatcatgg aggaggggaa
    11581 gctgttatcg tcgatacctt tttgtccgcc ccagccgccg cctacggcga tgttcagcag
    11641 cagatgaaac cgttgatcga acggccattc ggcaaagcct ttaccggtat tatggaagga
    11701 ggtatagacg tttccatcta cgaagaagcg gatctgttgg gtgtcccatt cgacggcgta
    11761 agtatggaag gcggtgtagg ggtcctttat aaagagccct tttgtctttt gtgtatggat
    11821 aacatggttg aagctttttg tgtggatgct gccgaagatg ctatcgggca tgaatcctac
    11881 attttccatg atatcgatct cgccgctggc gggccagccg ccatatttcc agtcggtggg
    11941 cagcatccag atagcgggcc agaggccccg gccggcgggc aggatggcgc gcacttcgat
    12001 gcggccgtag agccagtcgc ccttgaattt tgtgacgagc cgggcggagg tgtaggggcg
    12061 tacgatgcgg gtggtggcag tggcggtgga tatttgttgt ttgcgggcgg tgatgtagag
    12121 gtggccgttg cgaacgacgg cgttgtttgt gtcggcgttt gaataatatt cgagttcgtt
    12181 gttgccccag ccgttgccgc cgacatcata ggaccatttt gtggagtcgg ggaggccggt
    12241 gtagttgaat tcgtcggacc agacgagttt atattgagcc tgtgcgtctg cgacgcacag
    12301 gctgaggagt aatagggtga tgagcgttct cattccgagt aggcgagtgc gccggtgggt
    12361 tggaagttga aatattgccg ggcgttatag tagcagatat cccggacgat cttgccgatc
    12421 cattccaggt cgttgggtag ttcgccgttg gtgatctctt cgccgaagag gttgcagagg
    12481 atgcggcgga agtactcgtg ccgggggaag gaaaggaagc tgcgggagtc ggtgagcata
    12541 ccgacgaagc ggctgagcag gcccatattg gagagggcgt ttatctggcg ggtcatcccg
    12601 tccttctgat cgaggaacca ccagccggag ccgtattgta ctttaccggc gatggagccg
    12661 tcgttgaagt tgccgatcat ggtagcgaac agttcgttgt cggcggggtt gagattgtag
    12721 aggatggtcc gggccaattt gtcttccttg tcgaggcgat cgaggaagcg gccgagagcg
    12781 cgcccctggg agaagtcgcc gatggagtcc cagccggtgt cgggcccgag tttttgcgtc
    12841 atgcggctat tgttgttgcg cagggcaccg aggtgaaact gctggaccca ccctttttcc
    12901 cagtcccatt cggcgaagtg gacgagcatg gcggatttga atttccggcg ttcggtgtca
    12961 ttcaactctt tgccggccct taccttgttg aagatggctt ctacttccca gccggtgaat
    13021 tcttccacgt agatctcttc gaggccgtgg tcgctgaccg tgcagcccat ggaggcgaag
    13081 aagtcgtggc ggttctcgag cgcgaagaga taatcgttaa agttggagat ggcgacgttt
    13141 gtcacggact cgagtctttt tatatagccg ttgaactttg ccggatcgtc gacgttcatc
    13201 gcgttatcgg ggcggaaagc agggaggata gggatctcga agccttcctg tttcagctg
//
LOCUS       DATGFG010000546         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1180247, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000546 DATGFG010000000
VERSION     DATGFG010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180247"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VKQ52_10255"
     CDS             complement(<1..872)
                     /locus_tag="VKQ52_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HLZ87615.1"
                     /translation="MGRKLLLEKAGVPGIRGYEVYRREGGYASVEKALKNMTPDQVTE
                     EVKKSGLRGRGGAGFPTGMKWSFLAKPEGVPRYLVCNADESEPGTFKDRYLMEFLPHL
                     LIEGLIVSSYALGSKATYIYIRGEYAWIPEILEQAIAEAKANGWLGKNILGTGFDCEI
                     YVHTGAGAYICGEETALIESLEGKRGNPRIKPPFPAVKGVWDCPTVVNNVETLAAVVP
                     ILNIGGEEYAKIGVGKSTGTKLLSACGNINKPGVYEIDMTISVEEFIYSDEYCGGIAN
                     GKRLKACIPGGSSVP"
     gene            complement(920..1420)
                     /locus_tag="VKQ52_10260"
     CDS             complement(920..1420)
                     /locus_tag="VKQ52_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HLZ87616.1"
                     /translation="MIQFSDEKLKKVSEIIGRYPEGKQKSALLPVLHLAQEEFGGWLS
                     VEAMDYVATLLRIEPIEVYEVATFYSMYNLKPVGRYMFEVCQTGPCMLRGSDDIIDYI
                     KKKLGINVGETTPDGLFTLKTVECLGACGYAPMMQLGKHFREHLTPEKVDQIIEECRQ
                     TAAANN"
     gene            complement(1536..>2004)
                     /locus_tag="VKQ52_10265"
     CDS             complement(1536..>2004)
                     /locus_tag="VKQ52_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87617.1"
                     /translation="EFDIPVGSTGDCYDRFLVRNQEMWQSMRIIEQAYRKIQDMKGAE
                     AEVYHADAPEYYLPEKSDVYTKMEALIWHFKIVMGETEIPPGEVYSAVEGGNGEVGYY
                     LISDGGRTPYRLHFRRPCFIYYQAYSELTKGAMLSDAIIVMSSLNLIAGEMDA"
BASE COUNT          445 a          729 c          399 g          431 t
ORIGIN      
        1 ggaaccgacg atccgcccgg gatacaggcc ttcagccgct tcccattggc gatcccaccg
       61 caatattcat ccgaataaat gaactcttcc acggaaatag tcatatcgat ctcataaaca
      121 cccggcttat tgatgttccc gcaagccgac aacagcttcg ttcccgtcga cttgcccacc
      181 ccgatcttcg catattcttc tccgccgata ttgaggatcg gcaccacggc cgccagcgtc
      241 tcgacattat tcaccaccgt cgggcaatcc cacaccccct tcacggccgg gaagggcggc
      301 ttgatcctcg ggttaccccg cttgccctcg agcgactcga tcagcgcggt ctcttccccg
      361 cagatatagg cgcccgcacc cgtatgaaca tagatctcgc aatcgaaccc cgtccccagg
      421 atattcttcc ccagccagcc attggccttc gcctcggcaa tcgcctgttc gaggatctcg
      481 ggtatccacg catattcgcc ccggatatag atataggtcg ccttgctgcc aagcgcatag
      541 gacgaaacga tcagtccctc gatcagcagg tgcggaagga attccatcaa ataccggtcc
      601 ttgaacgtcc cgggctcgga ttcgtcggca ttacaaacaa ggtaacgcgg aactccttcc
      661 ggtttcgcca ggaagctcca cttcatcccc gtcgggaaac ccgccccacc gcgaccacgc
      721 aggccgctct tcttcacctc ttccgtcacc tggtctggcg tcatattctt cagggccttc
      781 tcgacactcg catatccacc ctcgcgccgg tacacctcgt acccgcggat ccccggaacc
      841 ccagcctttt ctaacaatag ctttctaccc atagtaaaaa ttcgtttata cgatcaattc
      901 ccgccccatc gcggcccaac taattattag cagccgcggt ctgccggcat tcttcgataa
      961 tttgatccac cttctccggc gtcagatgct cccggaaatg cttgcccagc tgcatcatcg
     1021 gcgcataccc acaggccccg agacactcca ccgtcttcag cgtgaacaac ccgtccggcg
     1081 tcgtctcacc cacattgatc cccagcttct ttttgatata gtcgatgata tcatcactgc
     1141 cccgcaacat acagggcccc gtctgacaaa cctcgaacat ataccggccc accggcttca
     1201 ggttatacat cgaataaaag gtagctacct catacacctc gatcggctcg atccgcaaca
     1261 gcgtagcgac ataatccatc gcctccacgc tcagccagcc cccgaactcc tcctgcgcca
     1321 ggtgcaacac cggcaacaaa gcactcttct gcttcccctc gggataacgg ccgatgatct
     1381 cactcacctt ctttaacttc tcgtctgaaa attgtatcat atcaaaacat gcattaatag
     1441 tctccgctca acaccctcca cagggccaat atctgctgcg ttccgcgcgc cccctccacg
     1501 gccaatatct gctccgcccc gcgacctccg cccggtcacg catccatctc ccccgcaatc
     1561 aaattcagcg aactcatcac aatgatcgca tcactcaaca tcgccccctt cgtcaattcc
     1621 gaatacgcct ggtaataaat aaaacagggc cggcggaaat gcaaccggta cggcgtccgc
     1681 cccccatcac tgatcaggta gtacccgact tccccattcc cgccttcgac ggcgctatac
     1741 acctcacccg gcgggatctc cgtctcaccc atcacgatct tgaaatgcca gatcagcgcc
     1801 tccatcttcg tatagacatc cgacttctcc ggcaaataat attccggcgc atccgcgtgg
     1861 tacacctccg cctccgcacc cttcatatcc tggattttcc ggtaagcctg ctcgataatc
     1921 cgcatcgact gccacatctc ctggttacgc accaggaagc ggtcataaca atccccggtc
     1981 gacccgacag gaatatcgaa ctcg
//
LOCUS       DATGFG010000547         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_608544, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000547 DATGFG010000000
VERSION     DATGFG010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608544"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VKQ52_10270"
     CDS             <1..1275
                     /locus_tag="VKQ52_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLZ87618.1"
                     /translation="AKLGARYAGMELSKDGKAIFVLAEGKILKIDAENGKQETVGING
                     EMVLREADEKAYIFDHAWRQMKEKFYVVNLNNVDWDFYYSAYKKFLPYINNDYDFAEM
                     LSEMLGELNVSHTGCYYFGAGRPTGDKTASLGIFYDHSYTGVGLRIAEVIAEGPLDKY
                     SGRIRPGVILEKIDGEAITDTVDHYKYLNRKAGKLMLLSLYDPGTGVRWEETTKPIGL
                     GEEEELLYKRWVNLRRKETDSLSKGRIGYVHVRAMDDQSMRTVFGEALGRNLEKDALV
                     VDTRFNGGGNIHEQLSDFLNGKKYFDIIPHGQYVGSEPYEKWVKPSIVVIGEANYSDA
                     HLFPAAYKLKGVGKTLGMPVPGTGTFVWWEEQIDPGLVFGMPMGGWRAPDGLFEEGHQ
                     LEPDILVRDEPKTLSMGHDQQIEAAVKELMKK"
     gene            complement(1325..2629)
                     /locus_tag="VKQ52_10275"
     CDS             complement(1325..2629)
                     /locus_tag="VKQ52_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87619.1"
                     /translation="MLSIPLRITVLACLFLICLVCCLIRIRLLRKKTRHLQLLADQQA
                     RLAEDALRTKSLFLANMSHEIRTPMNGITGMSSLLGQTALTREQRGYIDTIRSCTDTL
                     LAVINNLLDFSTMESGKIILEEKETDLRLCVEEVLEVYMARALKAGILLHSHIDEDVP
                     ARIITDGARLRQILINLIGNAIKFTEQGEIRIRILIPPTPMPPDTPPGHIQIGFEVKD
                     TGIGIDPEQFSQLFQSFSQVDSSVSRRYGGTGLGLTISDKLVQLMNGHIAVESKPGKG
                     STFTFTILAHPIEPKTMTPTTPTIPVLADKYPLCILLAEDNPINQQLALIILTKMGYN
                     PIVAFNGKEVLQHLEKGPFDLILMDIQMPEMDGLEATRLIRKGEGHQPVIIAMTANAT
                     KQDRDECLAEGMNDYLSKPVDLEELAYMLKKWGHLINTPHRV"
BASE COUNT          592 a          590 c         1091 g          742 t
ORIGIN      
        1 gccaaattgg gcgcgcggta tgcggggatg gagctatcga aggatgggaa ggcgatcttt
       61 gtgctggcgg aggggaagat actgaagatc gatgcggaga acgggaaaca ggagacggtg
      121 gggatcaatg gggagatggt gctccgggag gcggatgaga aggcctatat cttcgatcat
      181 gcctggcggc agatgaagga gaaattctat gtcgtcaacc tgaataatgt ggattgggat
      241 ttttattatt cggcgtataa gaaatttcta ccctatatca ataacgacta tgattttgcg
      301 gagatgctga gcgagatgct gggtgagctg aatgtctcgc ataccggttg ttattatttc
      361 ggcgccggaa ggccgacggg tgacaagacg gcgtcgcttg ggatctttta tgatcattcc
      421 tatacgggtg tgggtttgcg gatcgcggaa gtgatcgccg agggtccgct ggataaatat
      481 tccgggcgga tcaggcctgg ggttatcctg gagaagatcg atggcgaggc gataaccgac
      541 acggtggatc actataaata tctcaaccgg aaggccggga agctgatgct tttgtcgctt
      601 tatgaccccg gcacgggtgt ccgctgggag gagacgacga agccgatcgg tcttggggaa
      661 gaggaggaat tgttgtacaa gcgctgggtg aacctgcggc ggaaggagac ggattcgttg
      721 tccaagggaa ggatcgggta tgtgcatgtg cgggcgatgg atgatcagag tatgcgaacg
      781 gtgttcgggg aggcgcttgg cagaaacctg gagaaggatg cgctggtcgt cgacacgcga
      841 tttaacggcg gggggaatat ccatgagcag ctgtcggatt ttctgaatgg gaagaaatat
      901 ttcgatatca tcccgcatgg gcagtatgtg ggatcggagc cttatgagaa gtgggtgaag
      961 ccgtccattg tggtgatcgg tgaggccaat tattcggatg cgcatttatt ccccgcggcg
     1021 tataagctga agggtgtggg gaagacgctg gggatgccgg tgccgggtac gggtacgttt
     1081 gtctggtggg aggagcagat cgatccgggg cttgtatttg ggatgccgat ggggggatgg
     1141 cgggcgcccg atgggttatt cgaggagggg catcagttgg agcccgatat ccttgtgcgg
     1201 gatgaaccga agacgctgtc gatggggcat gaccagcaga tcgaggcggc tgtaaaggaa
     1261 ttgatgaaga aatagtttga gggcgccgcc ggggagtttg gtgggcgccg gggggtagtg
     1321 gctgttaaac ccgatggggg gtattgatga gatgccccca tttttttagc atataggcca
     1381 gttcttcgag gtctaccggt ttgctgaggt agtcgttcat gccttcggcc aggcactcgt
     1441 cgcggtcctg tttggtggcg ttggccgtca tggcgatgat aacgggttga tggccttcgc
     1501 ctttgcggat gaggcgggtg gcttcgaggc catccatttc cggcatctgg atatccatca
     1561 ggatgagatc gaaggggcct ttttcgaggt gttgcagtac ttctttccca ttgaaggcga
     1621 cgatggggtt gtagcccatt tttgtcagga tgatgagggc cagctgctgg ttgatggggt
     1681 tgtcctcggc cagcaggatg cagagcggat atttatcggc gaggacgggt atggtgggtg
     1741 ttgtgggggt catggttttt ggttcgatgg ggtgagcgag gatggtaaag gtgaatgtcg
     1801 agcctttgcc gggtttgctt tccacggcga tatggccgtt catcagttgt accagtttat
     1861 cggagatggt gaggcccagg ccggtgccgc cgtatctgcg gcttacggag gagtctacct
     1921 gtgagaagga ctggaagagt tggctgaact gttcgggatc gatgccgatg ccggtgtcct
     1981 ttacttcgaa gccgatctgg atatggccgg gcggggtatc cgggggcatg ggtgtcgggg
     2041 ggataaggat gcggatgcgg atctcgccct gttcggtaaa ttttatggcg ttgccgatga
     2101 ggttgatcag gatctgccgg aggcgggcgc cgtcggtgat gatacgtgcg gggacgtcct
     2161 cgtcgatgtg actatgcagc aggataccgg ctttcagggc acgggccata tagacctcga
     2221 gtacttcttc cacgcataac ctgagatcgg tttctttttc ttccagaatg atcttcccgg
     2281 attccattgt tgaaaagtcg agcaggttat tgatgacggc gagcagggtg tcggtgcagc
     2341 tgcggatggt gtcgatatag ccgcgttgtt ctcttgtgag ggcggtctgg ccgaggaggg
     2401 aggacatgcc ggtgatgccg ttcatggggg tgcggatctc atggctcata ttggccagga
     2461 agaggctttt tgtccgcagc gcgtcttcgg ccaggcgagc ctgctggtct gccaggagtt
     2521 gcaggtgcct ggtctttttc cttagcaggc ggatccggat caggcagcaa accagacaga
     2581 ttaggaaaag gcaggccaga actgttattc taaggggtat ggacagcatc ggcttgggtt
     2641 tcagagttca gagcgtaaca aaataggttc ataggacctc agggtctatg gttaacggta
     2701 caggatggct tgtattttgg ccttttggat ttatttgacc actatagggg tttttgctta
     2761 gccgggtcgc ccaggggagt ctaaaactga cggttagggc tacatgccct gtaagggtaa
     2821 aatttaggtg tgcctggccg ccgtttgggg gcagggagat gggttttgcg tttgagggcg
     2881 aggggttgga ttttgcgttt gagggccggg gttagacctc gtccaggcgg aaggggagtc
     2941 tccggatgcg cttgccggtt aggtcgaaga tggcgttgca gagggcgggc gccaggggtg
     3001 gtagtccggg ttcgc
//
LOCUS       DATGFG010000548         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_383607, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000548 DATGFG010000000
VERSION     DATGFG010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383607"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VKQ52_10280"
     CDS             <1..769
                     /locus_tag="VKQ52_10280"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HLZ87620.1"
                     /translation="SFGLNAGGSQKTKNNGEFGLKVSAYLDQVTMILPSEFEPAGAYR
                     VGRNGDLRHPDYGSSGRQTYTASFSFSQVINTRLQGSLLLDIVEQQGYLGLPFHRVYF
                     TDGTDSIEKLPSSRFKLPLGLRLNYFWGDHLILRGYYRYYADSWGLRSHTASLEASVK
                     VTPFFSVTPFYRYYVQTAADYFAPYAAHLKTDGYYSSNYALAGFNSSFFGAGIRLAPP
                     KGILYSHLKMMEIRYGHYTQTTDLVSDVISLNLQFRR"
     gene            complement(1076..1573)
                     /locus_tag="VKQ52_10285"
     CDS             complement(1076..1573)
                     /locus_tag="VKQ52_10285"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HLZ87621.1"
                     /translation="MTHTQTSATYKPILLIFAYILVITISIEAASASFDSMRWMNHFM
                     AAFFLVFSFFKFLDLSAFADSYSTYDIIAARWRGWGLIYPFVEAALGVSYLTGASPLF
                     TNIATFLVMGVSSIGVIRSVLRKQRIQCACLGAVFNLPMSTVTIIEDGVMIAMSATVI
                     GAMLL"
     gene            complement(1662..2240)
                     /locus_tag="VKQ52_10290"
     CDS             complement(1662..2240)
                     /locus_tag="VKQ52_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ87622.1"
                     /translation="MTHLYIKNMVCNRCIYTVRQIVEAEGLHPANITLGEVQLPAPPT
                     AEQLTAIRRKLHEFGFEILDDQKHRVIEKIKTIIIEHIHQHGPDTGKHPVFSKLLAAE
                     LHKDYSALSKLFSEAEGITIEKYIIQQKIEKVKEWLAYDELTLSEIADRLGYSSVAHL
                     SAQFKKTTGFTPSAFRKQKDHHRRPLDRVGKE"
BASE COUNT          518 a          574 c          758 g          604 t
ORIGIN      
        1 ttccttcggg ctgaatgcag ggggatcgca gaagacaaag aacaatgggg aattcgggct
       61 gaaggtgagc gcatacctcg accaggtgac gatgatcctg ccttcggagt tcgagccggc
      121 aggcgcctat cgggtcggtc gcaacggaga cctgcggcat ccggactatg ggagcagcgg
      181 gcgtcagacg tatacggcct cgttctcctt ttcgcaggtg atcaatacgc ggctgcaggg
      241 aagcctgctg ctggatattg tcgagcaaca gggatatctc gggctgcctt ttcaccgggt
      301 gtattttacg gatgggacgg attcgatcga gaagctgccg tcgagccggt ttaaattacc
      361 cttgggattg cggttgaatt atttctgggg cgaccatctc atcctgcgtg gctattaccg
      421 gtattatgcc gatagctggg ggctccggtc gcatacggcc agcctggaag cctcggtgaa
      481 ggtgaccccg ttcttctcgg tgacgccctt ctaccggtat tatgtacaga cggcggccga
      541 ttattttgcc ccgtatgccg cgcatctgaa gacagacggt tattattcca gcaattatgc
      601 ccttgcgggc ttcaacagca gtttcttcgg ggcgggcatc cggctggcgc cgccgaaagg
      661 tatcctgtac agtcacctga agatgatgga gatccggtat gggcactata cccagacgac
      721 agatctggtg tcggatgtga tctcgctaaa cctgcagttc aggagataag aacccatgat
      781 tgggggcgtg gagagtgatc ggctacagac atttgtgtat tttgactgca acgtggaata
      841 aaatgttgct tcccgtcgtt taataataca actttgtttt gttgatcgca cgcgtaaaac
      901 aagctgatcc ggcgaaagta gatttcccga tccacgtgag atcgccccgt taaagatccg
      961 taccccgcca gtacatccta tcgaaaaacc ggagcaacga tcctgaacca aaagttattg
     1021 tgccatgcat acggttttgc gagcccaccg atcggtgggc tcgctttttt ttgggttata
     1081 acagcatggc accgatgacg gtcgcgctca tggctatcat tactccatct tcgataatgg
     1141 tcaccgtgct catgggcagg ttgaacaccg cacccagaca ggcgcattgg attcgctgct
     1201 tcctgagcac cgaccggatg acgccgatgc tgctgacgcc catgacaagg aaggtggcga
     1261 tgttggtgaa taagggagat gcgccggtga ggtaggagac acccagggcc gcttcgacga
     1321 agggatagat cagtccccag ccgcgccagc gggcggcgat gatgtcgtat gtgctgtagc
     1381 tgtccgcgaa ggcggaaaga tccaggaatt taaagaagga aaagaccagg aagaaggcgg
     1441 ccatgaagtg gttcatccag cgcatggagt cgaaggaggc gcttgccgct tcgatgctga
     1501 tcgtgatgac caggatatag gcgaagatga ggaggatcgg tttataggtg gccgaggttt
     1561 gtgtatgtgt catgatcgga ttattttatc atacaaagtt agccccgggc ggtggcgggg
     1621 gtgttacagg attgtggaaa aggattgtat aattcggcgg atcactcctt ccctaccctg
     1681 tccagcggac ggcggtgatg gtccttttgt ttacggaagg ccgagggtgt gaagccggtt
     1741 gtttttttga actgggcgga gagatgtgcg acgctgctgt agccgagccg gtcggctatt
     1801 tcgctcaggg tgagttcgtc gtatgccagc cattccttca ccttttctat cttttgctga
     1861 atgatgtatt tttcgatggt gatgccttcg gcttcggaga agagtttgct gagggcggag
     1921 tagtctttgt ggagttcggc cgccaggagc tttgagaaga cggggtgttt gccggtgtct
     1981 gggccgtgct ggtggatatg ttcgataata atggtcttta tcttttcaat gacgcggtgt
     2041 ttttggtcat cgaggatctc gaatccgaat tcgtggagtt ttcgccggat ggcggtgagt
     2101 tgttccgctg ttggcggggc agggagttgg acttcgccga gggtgatgtt ggcgggatgc
     2161 aggccttctg cttcgacaat ctgtctgacc gtatagatac agcggttgca aaccatgttc
     2221 tttatgtaaa ggtgggtcat gggtgcgaag gtaaagcaga ttaccggatt gccccgcggc
     2281 ttactcgctg cgcaaggacc tcacgggatt ggccatcgcc gccttcaccg cctggtaact
     2341 gatcgtaagc agggcgatca ggagtgcgat gatcccggcg gagaggaaga tccagacgct
     2401 gatcctgatc cggtaagcaa agctctgcaa ccatttatcc atcagcatcc atgc
//
LOCUS       DATGFG010000549         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1375311, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000549 DATGFG010000000
VERSION     DATGFG010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375311"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VKQ52_10295"
     CDS             complement(<1..685)
                     /locus_tag="VKQ52_10295"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87623.1"
                     /translation="MKRTNLLYYALLAGVLLGYAACKKSFLTAQPEGTQLESNYYQSP
                     DEAFAGLVAAYNPLSWTVVSSYAPKMVLLTLASDEATAGGGSNSDNPGMQAWNTYTLN
                     AATPNVPPDLWSRNFTGIFRVNTMLQKVGSVPGLSSDLKTRYTAEMQFLRAYYYFDLV
                     REFGNVPLITAVLQQSQFFTQTQAKPADVYAQIEKDLNAAIPNLPATVDPATEGGRVT
                     KGAAQALLGK"
     gene            complement(705..>3391)
                     /locus_tag="VKQ52_10300"
     CDS             complement(705..>3391)
                     /locus_tag="VKQ52_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ87624.1"
                     /translation="LQGRTSGVTIAANSGQPGSSQQVRVRGTTSINASDPLYVVDGVP
                     LDGGGLDYLNPADIESIEVLKDAASQAIYGARAAAGVILVTTKKGKAGTMRVSYDGYT
                     GTQAPARKLHLLNAVQYATLRNESSLAGGKGIVFSNPASLGNGTDWQKQIFDNSASEM
                     NHQLSFSGGTDKSTFFTSLGYFDQNGIVASPISWYKRVTARFNGSHKINPWLNFGENI
                     GYSYIKNDGLGNTNSEFGGPLSSAINLDPVTPVVITDPTVLNNPTSIYNTEPVERDAL
                     GRPYAISQYVGQEETNPVAYIKTRQGNGYNWSHNIVANGYLGVTPIKGLELRTSIGTK
                     LAFWGSNSFTPIYYLNGSTSNPITNFYRDAENLFIWDWENTASYNRHFGLHNVTALVG
                     TSAWENNNGVGINGNFQYMPVTSLGQATPNYSLPNADKVANGYDNPVHHVSSEFARVV
                     YSYDDRYLLEGQIRRDGSTKFGDNNKYGYFPGGSVGWVVTREHFFPTTQALSFLKVRG
                     SYGVTGQDNISDFLYESTVSGGRNYTFGGNYGIGYSPNAPPNPDLKWEQTTQIDVGFD
                     AVVLQNFNVTLDLYNKKTTGMLMTEQLPLYVGSSGNPTGNIGDMSNKGIELEIGYNKK
                     IGEVQLGLKGNGSLLRNRVTYLGVTPYLVGATLQSSAYELARTAAGHPIGSFYGFKTL
                     GIFQTQADVSNYVNKSGQMIQPNAKPGDFKWADLNGDGAITANDRTFIGDPTPHYTYG
                     FTATAAWRGFDIVVFGQGAGGNMIFQGLRRLDIQTANYSTKALGRWTGPGSTNSNPRL
                     VDGDPNNNFTNPSAFYLEPGAYFRIKTLQIGYTLPQSLLRKWTLQKVRVYVSGYNLVT
                     FTHYTGYDPEIGGGSNTSIDRGFYPQARTLQAGLSVGF"
BASE COUNT          733 a         1031 c          880 g          747 t
ORIGIN      
        1 cctttccaag caacgcctgc gccgcaccct tggtgacccg cccgccttcg gtcgcaggat
       61 ccacggtggc cggcaggttg gggatcgccg cattcagatc cttctcgatc tgcgcataga
      121 catccgccgg cttcgcctgg gtttgcgtaa agaactgact ttgctgcagc acggccgtga
      181 tcagcggaac attgccgaat tcccgcacga ggtcgaaata ataataagcc cgcaggaact
      241 gcatttccgc cgtataacgg gtcttcagat cgctgctcag cccgggaaca ctcccgacct
      301 tctgcagcat cgtattgacg cggaagatac ccgtgaaatt gcggctccaa aggtccggcg
      361 gcacattggg cgtcgcagcg ttcagcgtat acgtattcca tgcctgcatg ccgggattgt
      421 cggagtttga cccgccgccg gcagtcgcct catccgaagc cagcgtcagc agcaccatct
      481 tcggcgcata ggagctcacc accgtccagg ataaagggtt ataggcagcc accagcccgg
      541 caaaggcctc gtccggcgac tggtaatagt tactttccaa ttgagtaccc tccggttgcg
      601 ccgtgaggaa actcttttta cacgctgcat aacccagcaa tacaccagcc aggagagcgt
      661 aatataaaag gttggttctt ttcatactgt tgttttttta atcgttagaa tcctacgctt
      721 aagccggcct gcagggtccg ggcctgcgga taaaaaccac ggtcgatact cgtattcgac
      781 ccgccgccga tctccggatc gtaaccggta taatgagtga aagtgaccag gttgtaacca
      841 ctcacataaa ccctcacctt ctgcagcgtc cacttgcgca gcaggctctg cggtaacgta
      901 tagccgatct gcagcgtctt gatgcggaaa taagcacccg gctccaggta gaaagcagag
      961 ggattggtaa agttgttgtt gggatcgcca tccaccagcc gggggttgct attggtagac
     1021 ccggggcccg tccagcgacc caatgccttg gtggaatagt tggcggtctg gatatccagc
     1081 cggcgcagac cctggaagat catattgccc ccggctccct ggccaaagac cacgatgtcg
     1141 aaaccccgcc aggcggcagt agccgtaaag ccatacgtat agtgaggagt agggtcgccg
     1201 ataaaggtcc ggtcattggc cgtaatagcc ccatccccat tgagatcggc ccacttgaaa
     1261 tccccgggct tggcattggg ctggatcatt tgaccgctct tgttcacata gttgctgacg
     1321 tccgcctggg tctgaaagat acccagtgtc ttgaaaccat agaacgatcc gataggatgc
     1381 cctgccgccg tacgcgccag ctcataggca ctggattgca aagtggcccc caccaggtaa
     1441 ggcgtgaccc ccaggtaggt gaccctgttc cgcaacaacg acccgttacc cttcagtcca
     1501 agctgcacct cgccgatctt cttgttataa ccgatctcga gctcgatccc cttattgctc
     1561 atatccccga tattgccggt cggattgccg ctggaaccga catacaaagg caattgctcg
     1621 gtcatcagca tccccgtcgt cttcttgtta tacagatcca atgtcacatt gaagttctgc
     1681 aggacaacgg catcgaagcc gacatctatt tgcgtcgtct gctcccattt cagatcggga
     1741 ttcggcggtg cattcgggct gtaaccgatg ccgtaattgc cgccgaaagt atagttgcgc
     1801 cccccgctca ccgtcgattc gtagagaaaa tcactgatat tatcctgacc cgtcacccca
     1861 tagctgccgc gcaccttcag gaagctaagc gcctgggtcg tggggaagaa gtgctcccgc
     1921 gtgacgaccc agcccacaga accaccggga aaataaccgt acttgttgtt gtcaccgaat
     1981 ttggtggacc catcccgcct gatctggccc tcgagaagat acctgtcgtc gtagctgtaa
     2041 accaccctcg cgaattcaga gctcacatga tgcaccgggt tatcataacc attggccacc
     2101 ttgtccgcat tgggcagact gtagttcggc gtcgcctgcc ccaggctggt caccggcatg
     2161 tactggaagt tgccgttgat gccaacccca ttgttattct cccaggcgct cgtcccgaca
     2221 agcgccgtga cattgtgcaa cccgaaatgc cggttatacg aagccgtatt ctcccagtcc
     2281 cagataaaga ggttctccgc atcccggtaa aaattcgtga tgggattgga ggtagagccg
     2341 ttcaggtaat agatgggcgt aaagctgttg ctcccccaga aggcgagttt cgtaccgatc
     2401 gaggtcctta gctccaaccc cttgatcggc gtcaccccga gataaccgtt cgcaacgata
     2461 ttgtgcgacc agttgtaccc gttgccctgc ctggttttga tataggctac cgggttggtc
     2521 tcttcctggc cgacatattg ggaaatggca taggggcggc caagcgcatc ccgctccaca
     2581 ggctccgtat tatagatact ggtggggttg ttcagcacgg tcgggtccgt gatcaccacg
     2641 ggcgtcaccg ggtccaggtt gatcgccgaa ctcagcggac cgccaaattc actgttcgta
     2701 ttcccgagtc catcgttctt gatataggaa tagccgatat tctccccgaa attgagccag
     2761 ggattgatct tatgcgaccc gttgaaccgg gcagtcaccc gcttgtacca ggaaatagga
     2821 gaagccacga tgccattctg atcgaaataa ccaagcgaag taaaaaaagt actcttatcc
     2881 gtgccgccgc tgaaactgag ctggtggttc atttcgcttg cactgttatc gaagatctgc
     2941 ttctgccagt cggtgccgtt gccaagcgac gcaggattgc tgaagacgat ccccttgccg
     3001 ccggcaagcg aggattcatt ccgcagcgtg gcatattgca cggcattcag caaatgcagt
     3061 ttcctcgcag gcgcctgcgt accggtatag ccgtcatagc tcacccgcat cgtccccgcc
     3121 ttaccctttt tcgtagtcac caggataacc ccggcagccg cacgggcccc gtagatcgcc
     3181 tggcttgccg catccttcaa cacttcgata gactcgatat cggccgggtt cagataatcc
     3241 agcccgcccc catccagcgg caccccatcc accacataca acggatcgct ggcattgatg
     3301 gacgtcgttc ccctcacccg cacctgctgc gaagaccccg gctgccccga attggcggca
     3361 atggtcacac cggacgtgcg cccctgcaag g
//
LOCUS       DATGFG010000550         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_59343, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000550 DATGFG010000000
VERSION     DATGFG010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59343"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1538
                     /locus_tag="VKQ52_10305"
     CDS             168..1538
                     /locus_tag="VKQ52_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87625.1"
                     /translation="MPTDESLIRQVKKLFEEKTGWGDSEKWSNQDFLQLGELIREETG
                     VTISHVTLKRIWGKVKYESLPNTHTLNTLVQYLGFENWRDFSVRQTAPEPKPEEVVML
                     RKEPERPGGNGPEQDALLAGAPVKEAEGVKQEEPVGERRWRKVVWMVAPLAVIMLVLL
                     FLKGQQPPPQARDYSFSSKKTVSKGLPNSVIFDYDATRSPDDSVVIQQSWDTTRRVKV
                     PRNGHQYTSVYYYPDFYHATLQVHGHVVKTHSLLIQTDGWLPLVEQDPVPVYFKTHEA
                     ISGGKMSLSLDQIRSKNILLQPSPPTVMFANVRDFGEIYSDHFTFETSLRNDYAEGSA
                     VCQETRIYLLCEGTAIWVPLIAKGCVSTVDLYFTYFYTSGKREDLSSLGVNFSDYIKL
                     RIESDSGKAKILINDQLAYTVPRHILHSKIIGILYRFKGTGSVDYVSLGNGKTEYSDN
                     FSFNDK"
     gene            complement(1525..2679)
                     /locus_tag="VKQ52_10310"
     CDS             complement(1525..2679)
                     /locus_tag="VKQ52_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HLZ87626.1"
                     /translation="MTSIILLLTAVITVLSRCVSTPPAPPDPRGEAFAGSTACLDCHH
                     DITNSYLHTAHALTSRPATAQSIKGRFPAEYDYGPHGRVLLQRYDSNFYQTAVHGQRT
                     EAHRFDIVIGSGRKAQTYLYWQGDAAYELPVSYFVTGASWANSPEFPADSIWFGRAIA
                     TECFECHASFIRNKPAVHTDAFHQTDQFARTTLLYGIDCERCHGPAAQHVAWQTKHPL
                     EKQPRFITRLSTLTRQQRLDACAVCHSGIHTEQLSLFRFRPGDAFSAYYYAEPVRNYD
                     TATMDVHGNQYQLLLSSECFKRSTNLQCNTCHDPHRQERESMTVFVRRCLSCHSPLPP
                     HKGMDSALVLTKCIDCHMPARPSGVITLQTAAGQKPVPDLIRTHRIAIYH"
     gene            2792..>3031
                     /locus_tag="VKQ52_10315"
     CDS             2792..>3031
                     /locus_tag="VKQ52_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HLZ87627.1"
                     /translation="MPKGKTKKVKEASTEEKIMEAARKLFTQRGFAATRTRDIAEEAG
                     INLALLNYYFRSKQKLFDIVMMENFRRFLGGMSANF"
BASE COUNT          671 a          691 c         1037 g          632 t
ORIGIN      
        1 cgctgatcag gcaaagcagg aggaggaggc gcagcataaa tttgttaaat tgcttatata
       61 aagttgagca ggctgcaagg aaaaagcgaa tcattcccaa aacatgttgg tacaagttgg
      121 tacaaatccc ggcggcaggc gcaacttcaa ctaattttac agcatctatg ccaaccgatg
      181 aaagcctgat ccggcaggta aaaaaattgt tcgaggaaaa gaccggctgg ggggatagcg
      241 agaagtggag caaccaggat ttcctgcaac tgggcgaact gatccgggag gagaccgggg
      301 ttacgatcag ccatgttacc ctgaaacgca tttgggggaa ggtgaagtac gagagcctgc
      361 ccaataccca cacgctcaat accctggttc aatatctcgg gtttgagaac tggcgggatt
      421 tcagcgtccg gcaaaccgcc cccgagccaa agccggaaga ggttgtcatg ttgaggaagg
      481 agccggagag gccgggaggg aatggcccgg agcaggatgc cttgttggcg ggggcgccgg
      541 ttaaggaagc ggagggggtt aaacaggagg agccggtagg agaacggcgg tggcggaagg
      601 tcgtctggat ggtggcgccg ctcgcggtga tcatgctggt gctgctgttt ttgaagggcc
      661 agcagccgcc gccccaggca agggattatt ctttcagcag caagaagacg gtgagcaagg
      721 ggttgcccaa ttcggtgatc ttcgattatg acgcgacacg gagtcccgac gactccgtcg
      781 tcatccagca gagctgggac acgacgcggc gggtaaaggt tccccgcaat ggtcatcagt
      841 atacttcggt gtattattac ccggattttt atcatgccac gctgcaggtt catgggcatg
      901 tggtgaagac acacagcctg ttgatccaga ccgatggctg gctgcccctg gtggagcagg
      961 acccggtgcc cgtgtatttc aaaacgcatg aggccatcag cggggggaag atgagcctgt
     1021 ccctggacca gatcaggagc aagaatatcc tgctgcagcc atcgccgccc acggtgatgt
     1081 ttgccaatgt gcgggatttt ggcgagatct attccgacca ttttaccttc gagacctcgc
     1141 tacggaacga ttatgcggaa ggatcggccg tttgtcagga gacgcgcatc tacctgcttt
     1201 gcgagggaac ggccatctgg gtaccgctta tcgccaaggg ctgcgtgtcc acggtggacc
     1261 tttacttcac gtatttctat accagcggga aacgggaaga tctttcttcg ctgggcgtca
     1321 acttttccga ctatatcaag ctgcggatcg agtcggacag cggcaaggca aagatcctta
     1381 tcaacgatca attggcatat accgttccgc gccatatcct gcacagtaag atcatcggta
     1441 tcctctaccg gtttaaggga acgggttcgg tggattatgt ctccctcggg aacgggaaga
     1501 ccgaatactc ggacaatttt agtttcaatg ataaatagcg atgcggtgtg tccggatgag
     1561 atcgggcacg ggcttttgtc cggcggcggt ctgcagggtg atgacgcctg aggggcgggc
     1621 gggcatgtga caatcgatgc atttggtcag gacgagggcg gaatccatgc ccttgtgcgg
     1681 cggcagggga ctgtggcagc tgaggcaacg ccggacgaag acggtcatgg attcgcgttc
     1741 ctggcggtgc gggtcgtgac aggtgttgca ctggagattg gtcgatcgct tgaagcattc
     1801 gctgctgagg agcaactggt attggttgcc atggacgtcc atggtggcgg tgtcgtaatt
     1861 gcgaaccggt tcggcatagt aatacgccga gaaggcgtcg ccggggcgaa agcggaacag
     1921 ggagagctgt tcggtgtgga tgcccgaatg acagacggcg caggcgtcga ggcgttgctg
     1981 gcgggtgagg gtggagaggc gggtgatgaa gcggggctgt ttttcgagcg gatgttttgt
     2041 ctgccaggcg acgtgctggg cggcggggcc gtgacagcgt tcgcagtcga tgccatagag
     2101 cagggtggtc cgggcgaact ggtccgtctg gtgaaaagca tcggtgtgca cggccggttt
     2161 gttgcggata aaggaggcat ggcattcgaa acattcggtg gcgatggcgc ggccgaacca
     2221 gatgctgtcc gccgggaact cagggctgtt ggcccaggag gcgcccgtca cgaaatagga
     2281 gaccggcagt tcataggccg cgtcaccctg ccagtacaaa taggtctggg ccttgcggcc
     2341 ggagccgatg acgatatcga agcgatgggc ctcggttctt tggccgtgga cggccgtctg
     2401 gtagaagttg ctatcgtagc gttggaggag caccctgccg tgggggccat agtcgtattc
     2461 cgcggggaat cggcctttta tcgactgggc ggtggcgggg cgggaggtga gggcatgggc
     2521 ggtgtggagg tagctattgg tgatgtcgtg gtggcagtcg agacaggcgg tgctgcccgc
     2581 aaaggcttcg ccgcgggggt cggggggtgc gggcggggtg ctgacacagc gggaaaggac
     2641 cgtgataacg gcggtaagaa ggaggattat ggatgtcaaa catctgtata gcaagctctc
     2701 tacgaattgg ttaagggaag gtagtccaat ttgttttaat catttgatta aaatacttgt
     2761 ttaaatcaga aacgcgtagt agctttgtgt catgccaaag gggaagacaa agaaagtgaa
     2821 ggaagcatcc acggaggaga agatcatgga agcggcgcgc aagctgttca cccagcgggg
     2881 gtttgccgcg acgcggacga gggatatcgc cgaggaagcc gggatcaacc tggccttgtt
     2941 gaactactat ttccgtagca aacagaagct gttcgatatt gtgatgatgg agaatttccg
     3001 gcggttcctg ggcgggatgt cggccaattt c
//
LOCUS       DATGFG010000551         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_392121, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000551 DATGFG010000000
VERSION     DATGFG010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392121"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2610
                     /locus_tag="VKQ52_10320"
     CDS             <1..2610
                     /locus_tag="VKQ52_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ87628.1"
                     /translation="LPAIGCSATGREVTIRPAAGAGQLTISSANATTTLDLNNGSHYI
                     LDGRPGGTDTTHLLVIGNTNTAASAIRLINGAGYNELHYLDIQGVTTSGASGVLFFAG
                     SDSALGNARNGIDSCTISAGATPPLYGIYGLDWQGPKNKGNRINACNIVNVLGTISND
                     GCGICFPVGYDSLIITNNSLYQTADRNTPPTGANVNQNYTGIRVGSSYDGGHLISGNF
                     IGGTAPKAGGDPMRLFQCHQFYGIKAICNTGAAMATQILDNTISNISWTNPNLGVYLI
                     YLGDFNGSTFNGRVSGNILGSTNGAATLVVTNPTYSSSNSVQFNGIWYLANAMTDPLI
                     ISDNHISGIAADANTPGSFYGMAITLNGYYHGSYYIRDNYIGDDIYHDNIWFQGAYAV
                     NGIAITQSQYPDTLVVTGNHIANMMSGSPYGNTSGDLVGINCYGASCGWRRIDSNTIH
                     DIYTVSGTAGDFYDIYYVGNNQPNTGIFTTNLSISGNNIYNSGFGMNTGGPSIYYQGG
                     ICLVNSAYGTMQATVSHNRIEGLYLPDTAASRILTFTPQLEVGMEFNNAGGGTITVDN
                     NMVRMGMGTGSRYQGGTVFGIWDLGGNNRYLHNTVYIQDYQMANEGGAIAFTSAEHSS
                     GSTVVMNNIFSGSKPANPKSYGLIDRPVSGLSFQCDYNIYYPGDTSASGARLYAWDST
                     FAQWQASTGFDAHSRLTNPLLVRPEGAWQDLDLHLQHGTPAESAGTASYTTLLDYDGQ
                     VRAQLTPVDIGADADNFNGVHVPPPTDTTTHDTTSAPRPVPTDSMGLVRPNGNPFSTT
                     LTLLIASPVTGTAQISIYNFSGNLLFTLPVSVIPGDNTVQIPMAQLPDGNYLVKVDIG
                     GKHETIQVMKKSN"
     gene            complement(2783..3346)
                     /locus_tag="VKQ52_10325"
     CDS             complement(2783..3346)
                     /locus_tag="VKQ52_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87629.1"
                     /translation="MKRTIFHLVVLAALVAGTAGVASAQSASGQGAAKGVSGKGAPEK
                     NSAAKGAAHGASADPACMQNSAARGVSAQTASAASASATGAAFPRTKDFFSKKGRRLF
                     TIAYYGEKELPRDIRAIVKPEYYDYTILTVEEVRLDGRSIYFIDMEDDTTLKTVRIAD
                     GEMELVRTLHRGDLDARPQDKAIVAGN"
     gene            3607..>4338
                     /locus_tag="VKQ52_10330"
     CDS             3607..>4338
                     /locus_tag="VKQ52_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ87630.1"
                     /translation="MTLSAVLLYSPQRRHRLTRHLVLWVLYAGYFTMQSYFPTGRVTG
                     ISSHFTRIALLSTAAYFPFCFLAAYLLLYYLYPRFLQRGQVLRFLLAFGALFLLGIVI
                     NAFAGVFFYRFSGREKVGLGARMGLGFHNTTIALITGLFILGLRLGRNAWLQRAANLR
                     LAAQKARAELQLLKTRVDPDFLFTTLDRLRAKVDSGDPGAPAAILQLSETLSGILYEG
                     EGELAFTANELPRYSQEESVFRQPAT"
BASE COUNT          898 a         1488 c         1138 g          814 t
ORIGIN      
        1 ctcccggcca tcggttgttc cgccacggga cgcgaggtca cgatccggcc cgccgcgggc
       61 gcgggccaac tcaccatcag cagcgccaac gccaccacca ccctcgatct caacaatggt
      121 tcgcattata tcctcgacgg ccgccccggc ggcaccgaca ccacgcacct gctggtcatc
      181 ggcaatacca acacagcggc cagcgctatc cgtctcatca acggcgccgg ctacaacgag
      241 ctgcactacc tcgacatcca gggcgtgacc accagcgggg ccagcggcgt cctcttcttc
      301 gcgggcagcg actccgccct gggcaatgcc cgcaacggca tcgactcctg tacgatctcc
      361 gccggcgcta ccccgccgct ctatggcatc tacggcctcg actggcaggg gccgaagaac
      421 aaaggcaatc gcatcaatgc ctgcaacatc gtcaacgtcc tcgggacgat cagcaacgac
      481 ggctgcggca tctgctttcc cgtgggttat gacagcctga tcatcaccaa caacagcctc
      541 tatcagacgg ccgaccggaa taccccgcct accggcgcca acgtcaacca gaactacacc
      601 ggcatccgcg tgggctccag ctatgacggg gggcatctga tcagcggcaa cttcatcggc
      661 ggcaccgcgc caaaggccgg cggggacccc atgcgcctgt tccaatgcca ccagttctat
      721 ggcatcaaag cgatctgcaa caccggtgcg gcgatggcta cccagatact cgacaacacc
      781 atcagcaata tctcctggac gaatcccaat ctgggcgtgt acctcatcta cctgggagac
      841 ttcaacggct ccaccttcaa cggccgggtc tcgggcaata tcctcggcag caccaacggc
      901 gcggccaccc tcgtcgtcac caaccccacc tactcgagca gcaattcggt acagttcaac
      961 ggcatctggt atctggccaa tgctatgacc gatccgctca ttatcagcga caaccatatc
     1021 agcggtatcg ccgccgacgc caacactccc ggctccttct atggcatggc gataacgctc
     1081 aacggctact atcacggcag ctattatatc cgcgataatt atatcggcga cgatatctac
     1141 cacgacaata tctggtttca gggagcctat gcggtgaatg gcatcgccat cacacaaagc
     1201 cagtaccccg acacccttgt cgtgacgggg aaccacatcg ccaacatgat gtccggctcc
     1261 ccctatggaa atacttccgg cgatctggtc gggatcaatt gctacggcgc gtcttgcggg
     1321 tggcgccgca tcgacagcaa cacgatccac gatatctaca ccgtctcggg cacggccggc
     1381 gatttctatg acatctatta cgtgggaaat aatcaaccga acacgggcat atttaccacc
     1441 aacctcagca tcagcggcaa caacatctac aactcgggct tcggcatgaa caccggcggt
     1501 cccagtatct actaccaggg cgggatctgt ctcgtcaaca gcgcttacgg caccatgcag
     1561 gccacggtaa gccataaccg catcgagggg ctctaccttc ccgacaccgc tgccagccgt
     1621 atcctcacct tcacgcccca gctggaggtg ggcatggaat tcaacaacgc aggcggcgga
     1681 acgatcacag tggacaacaa catggtccgc atgggcatgg gcaccggcag ccgctaccag
     1741 gggggaaccg tgtttggcat ctgggatctg ggcggcaaca accgctacct gcataatacg
     1801 gtctacatcc aggattacca gatggcgaat gagggtggcg ccatcgcttt tacgagcgcc
     1861 gagcattcat ccggctcgac ggtggtcatg aacaatatct tttccggcag caaaccggcc
     1921 aacccgaagt cctatgggct catcgaccgg ccagtgtcag gcctctcctt tcaatgcgat
     1981 tacaacatct attatccggg cgatacctcg gcaagcgggg cccgtctcta cgcgtgggat
     2041 tccacgttcg cgcaatggca ggcctcgacg ggcttcgacg cccactcccg gctgaccaat
     2101 cccttgctcg tgcgacccga aggcgcctgg caggacctcg atctgcactt gcaacatggg
     2161 acgcccgcgg aaagcgcggg gactgcttcg tatacgacgc tgctggatta tgacggccag
     2221 gttcgcgccc agctgacacc ggtggacatc ggcgccgacg cggacaactt caacggcgtc
     2281 cacgtcccgc caccgacgga taccactacc catgacacca cttccgcccc gcgccccgtc
     2341 cccaccgact cgatgggact ggtacggccc aacggcaacc cgttctccac gacactcacc
     2401 ctactgatcg catcacccgt caccggcacg gcgcagatca gcatctacaa cttctccggc
     2461 aacctgctgt tcaccctgcc ggtgtccgtt atcccggggg ataatacggt gcagataccg
     2521 atggcgcagc ttcccgatgg gaattatctg gtgaaggtcg acatcggcgg caagcacgag
     2581 acgatacagg tgatgaagaa aagtaattag gtgcggcggc ctccggatcc accactcgca
     2641 cccaccaccg cccgccgacc atggccagcc tcgaccccct gcatctcgga cccaccgcca
     2701 ccgcccgtcg tgccaacgcg cggacccgct gcacctcgga cccaccgccg ggcaccccaa
     2761 cggacatggg caaccgccga tctcaattac cagccactat ggccttgtcc tgcggacggg
     2821 catcgaggtc tccccggtgg agggtccgca ccagttccat ctcgccatcg gcgatcctaa
     2881 cggtcttcag cgtcgtgtca tcctccatgt cgatgaagta gatcgacctt ccatccagcc
     2941 gcacttcctc caccgtcagg atggtataat cgtaatactc cggtttgacg atcgcccgga
     3001 tgtcgcgggg caattccttc tcgccgtagt aggcgatggt gaacagacgc cggcccttct
     3061 tgctgaagaa gtcctttgtc cggggaaatg cggcgcccgt ggcggaagca gacgcggcgg
     3121 aggcagtctg cgcggagaca cccctggcag cggaattctg catgcaggca ggatcggcag
     3181 aagcaccgtg agccgcaccc ttggcagcgg aattcttctc cggtgcaccc ttgccggaga
     3241 cacccttggc agccccctgg ccggaggcac tctgcgccga agcgacaccg gcggtccctg
     3301 caacaagggc ggccaaaaca acgagatgga aaatagtcct tttcatatat ttacttttta
     3361 ggttgataaa gaggaggccc tggaaaaagc ctccggtttg ctttatgaac atccatcatt
     3421 ggatgacaac ccaaaactac tccgccgctt ccccctccgg acgcttcttc gaccgttacc
     3481 cccttcccgc ccctattcga cggtcacggg tcgcccgccg ccactcatcg tcgacgaatg
     3541 gcattcgtcg aaaaacggtt cgccttctac cgcatctctc tacctttaac aaggcaaaac
     3601 aaaggcatga ccctcagcgc agtactacta tattctccac agcgtcgcca ccggctgaca
     3661 agacatctcg tcctttgggt gctctatgcc ggctatttca cgatgcagag ctatttcccc
     3721 accggccgcg tcaccggcat cagcagtcac tttacccgca tcgcactcct cagcacggcg
     3781 gcgtactttc cgttctgctt cctggcggcc tatttgttat tgtattatct ctacccgcgc
     3841 ttcctgcaac gcggccaggt cctgcggttc ctcctcgcct tcggggccct gttcctcctg
     3901 ggcatcgtga taaatgcttt cgccggggtg ttcttctacc ggtttagcgg cagggagaag
     3961 gtgggcctcg gagcccgaat gggtctgggc tttcataaca ccaccatcgc tctcattacc
     4021 ggtctgttca tcctggggct gcgcctcggc aggaacgcct ggttgcagcg tgcggccaat
     4081 ctgcggctgg cggcccaaaa ggcaagggcg gagctccagt tattaaagac gcgggtcgat
     4141 ccggattttc tcttcaccac cctcgaccgc ctgcgagcca aggtcgatag cggggacccc
     4201 ggcgcgccgg ccgccatcct ccaactgtcg gagaccctga gcggcatcct gtatgaagga
     4261 gagggggagc tggcctttac tgcgaatgag cttccacgtt atagccagga ggagtcggtc
     4321 ttccggcaac ccgccacg
//
LOCUS       DATGFG010000552         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_347452, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000552 DATGFG010000000
VERSION     DATGFG010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347452"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VKQ52_10335"
     CDS             complement(<1..481)
                     /locus_tag="VKQ52_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HLZ87631.1"
                     /translation="MSLYAQRGVSAQKEEVHQAVEKLDKGLYPNAFCKIYPDYLGGDA
                     SWVNLMHADGAGTKSVLAYLYWKETGDSSVWKGIARDAIAMNLDDLLCVGIYDNLLFS
                     STIDRNKRNIPGEVLSEIINGSQEFFDELGGYGVGIRYLGGETADVGDVVRTIAVNGT
                     "
     gene            567..1520
                     /locus_tag="VKQ52_10340"
     CDS             567..1520
                     /locus_tag="VKQ52_10340"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HLZ87632.1"
                     /translation="MDIYRHLSQLPVFQRSVITIGTFDGVHTGHAHILRQLREEAARI
                     DGETVIITFFPHPRKVVRGGAEEVRLINTLDEKIQLLSWQQIDHLVIVPFTEAFSQLT
                     AEEYIKDFLLAKFHPHTVIIGYDHRFGKGRQGDYHLLEQFSASEGFVLQEIPVHLLNE
                     VSVSSTRIREAILRADIDTANQLLGYPFFFSGKVVKGNRLGRTLGYPTANLDPQNSEK
                     LIPADGVYAVEAELLPAAGPLFSGPRIQGMMGIGIRPTINGRDRTIEVNLFDFDEDIY
                     DRELRVFVKKFLRPELKFNGLEELKGALAQDKLDTLAALNP"
     gene            complement(1510..2508)
                     /gene="holA"
                     /locus_tag="VKQ52_10345"
     CDS             complement(1510..2508)
                     /gene="holA"
                     /locus_tag="VKQ52_10345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HLZ87633.1"
                     /translation="MSVEKILGDWKKLKFKPVYWLEGEEEFYIDELVNYAEHHILSEA
                     EAGFNLTVFYGRDADWAAVINACRRYPMFAERQVVLLKEAQQMRDIDKLEAYIDKPLV
                     STVFVVAYKEKKVDGRSKLAKLLKEKAELYTTKKLYDNQLPEWTTELVRQKGYTMTQK
                     ALLLLVDHIGNDLSRINNEVEKLLVNLSGRKEITEDDIERYVGVSKEYNVFELQDAFA
                     KKDRVKAIRIIQYFEGNPKAGPIQLILPSIYNFFSKAYMIFGQPARDEKGVAAAIGVN
                     PYFVKDYLAAVRNYNYEGIEHGLLLLHAYNLKSVGVGSAGVEDASLLKELVVKIMG"
     gene            2677..3657
                     /locus_tag="VKQ52_10350"
     CDS             2677..3657
                     /locus_tag="VKQ52_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87634.1"
                     /translation="MSTTTNYPSATPQSQPPKKDYKNLVIGLLAAGFLGTWGYLLWNN
                     NKQEQIQTQQQTQIAKVSDEKGQLQKNFDDALVRLDSLTGTNNKLSATVNERDGQVAK
                     LKGQIRGLLSKQHLTEAEKKKAEELIKELNDKVSNLEQQVAQLTQDNQQLTADKTQLT
                     ADKEKLTNDLTTTNTAKQDLEKKVDVASTLNASNISVTPMHEKSNGQDKETTTAKRVN
                     KLVIGFDVANRIAQTGQTDVYVCITGPDGKPISVPALGSGTFTTRDEGDKMYTAKVPV
                     DFEAGKTKSVQFAWKQNSQFQKGNYKIEIYHNGFKIGEGTRELKKGGLFS"
     gene            complement(3731..>4471)
                     /locus_tag="VKQ52_10355"
     CDS             complement(3731..>4471)
                     /locus_tag="VKQ52_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87635.1"
                     /translation="QEARGLIEYNPFKSPLSDTSLITRTMILVNTLSFNRSDPRWGFD
                     LSNTRNGGKTLLTYGYERRQTNEWSLRTRLNMNKAIALTGVFRNGVNDYNNSSTNFDS
                     ANYNLKQWSAEPGLTYTRRSNLRIGLSCRLTSKQNSPVWGGQKYTSEAWNTDFKYNIL
                     QSTSIQGKFTMSSIVYTAGGGKSPSTTSAVSYTILEGLQPGKNYLWNLDLTKKLGNSL
                     ELSLQYEGRKAGTTGVVHTGRAALRALL"
BASE COUNT         1109 a         1408 c         1015 g          939 t
ORIGIN      
        1 tggtcccatt gacagcgatc gtccgcacca cgtcgccaac gtccgccgtc tccccgccaa
       61 gataccggat acccacacca tatcccccca gctcgtcgaa gaattcctgc gagccattga
      121 tgatctccga caacacctcc cccgggatat tccgcttgtt gcgatcgatc gtggaagaga
      181 acaacaggtt atcatagatg ccgacacaga gcaggtcatc caggttcatc gctatcgcat
      241 cccgcgcaat ccccttccag acactgctgt caccggtctc tttccagtac aggtaggcaa
      301 gaacactctt cgttccggcc ccatcggcat gcataaggtt tacccaggac gcatcccccc
      361 ccaaatagtc gggatagatc ttgcaaaaag cgttcgggta gagcccctta tccaactttt
      421 caacggcctg gtgaacttct tctttctgag cggagacccc gcgttgggcg tagagtgaca
      481 taggcggcaa aggtaaaaga taaatcgctt agatttgcaa caaaacgggc cacgggtccg
      541 ttggccgaag gcccagattt taaacaatgg atatctatcg tcatctctct caattacctg
      601 tctttcagcg ctcagtaatc accatcggaa cctttgacgg cgtccacacc ggccacgccc
      661 atatcctccg gcaactcagg gaggaagccg cccgcatcga cggcgaaacc gtcatcatca
      721 ccttcttccc ccacccccgc aaagtggtca ggggcggcgc cgaagaagtc cgcctgatca
      781 acaccctcga cgaaaagatc cagctccttt cctggcaaca gatcgaccat ctcgtcatcg
      841 tccccttcac cgaagccttc tcccagctga cggccgagga atatataaaa gacttcctcc
      901 tggcgaaatt tcacccgcat accgtgatca tcggctacga ccatcgcttc ggcaaaggcc
      961 gccagggcga ctaccacctg ctcgaacagt tcagcgcatc ggaggggttc gtcctccagg
     1021 agatccccgt ccacctgctg aacgaggtat ccgtcagctc tacccgcatc cgcgaagcca
     1081 tcctccgcgc cgatatcgat acggccaacc agctcctcgg ctaccccttc ttcttctcgg
     1141 gaaaggtggt aaaaggcaac cggctgggcc gcaccctcgg ctacccgacc gccaacctcg
     1201 atccgcagaa cagcgaaaaa ctgatccccg ccgacggcgt ctacgccgtg gaagccgaac
     1261 tcctccccgc tgccggcccg ctcttctccg gcccccgtat ccagggcatg atgggcatcg
     1321 gcattcgtcc gaccataaac ggcagggatc ggaccatcga ggtcaacctt ttcgattttg
     1381 atgaagatat ctatgacagg gaactgcgcg tcttcgtgaa gaaattcctc cgccctgaac
     1441 taaagttcaa cggcctggaa gaactaaaag gcgccctcgc acaggacaag ctcgacacgc
     1501 tcgcagcgct caacccatga tctttaccac caattccttc aacagcgacg catcctcgac
     1561 accggcactg ccaaccccca cgctcttcag gttataggcg tgcagcaaca gtaacccgtg
     1621 ctcgatgccc tcgtaattgt aattacgcac cgccgcaaga tagtccttga caaaataagg
     1681 attcaccccg atggccgcgg caaccccttt ctcgtcccgg gccggctggc caaagatcat
     1741 ataggccttg gaaaagaaat tatagatgga aggcaggatc agctggatcg gccccgcctt
     1801 gggattcccc tcgaaatact ggatgatccg gatcgccttc acccggtcct tcttcgcaaa
     1861 agcatcctgt aactcgaata cattgtactc cttgctcacc cccacatacc gctcgatatc
     1921 gtcttccgtg atctccttcc tgccggaaag gttcaccagc agcttctcca cctcgttatt
     1981 gatccggctc aggtcattgc cgatatggtc caccagcaat aacagggcct tctgcgtcat
     2041 ggtgtatccc ttctgccgga caagctccgt cgtccattcc ggcagctggt tatcgtacag
     2101 cttctttgtc gtatacagct ccgccttttc ctttaacagc ttggctaact tgctccgccc
     2161 atccaccttc ttctccttat acgccaccac gaacaccgtc gaaacaagcg gtttgtcgat
     2221 atacgcttcc agcttgtcga tatcccgcat ctgctgggcc tccttcaata gcaccacctg
     2281 ccgctcggca aacatcgggt atcgccggca ggcattgatc acggccgccc aatcggcatc
     2341 cctgccataa aatacggtca ggttgaaccc cgcctccgcc tccgagagaa tatgatgctc
     2401 cgcatagttc accagctcat cgatatagaa ctcctcctcc ccctccagcc agtaaacagg
     2461 cttgaatttt aattttttcc agtcaccgag gatcttttca acagacatcc ttgttgtttt
     2521 ttgtgaggca atattacata aatctttagg aatcaggcgt tgaacgcaat aaaaaggttt
     2581 ttggcacggt tttagactaa tggcaagtgt aaccgcaaaa tcggttagca ggtttcggaa
     2641 aaccatttct ataacatctg aaaaaaacaa taagctatga gtacgacaac caactatccc
     2701 tcagcaactc cccaaagtca gccgccgaag aaagattaca agaatctggt gatcggtctg
     2761 ctggccgccg gctttcttgg gacatggggc tatttgctct ggaacaacaa caaacaggag
     2821 cagatccaaa cccagcagca aacgcaaatt gctaaggttt cagacgaaaa aggacaatta
     2881 cagaagaatt tcgacgatgc tctcgtgaga ctggatagcc tcaccggcac caacaacaaa
     2941 ctctctgcca ccgtcaacga aagggatggc caggtagcaa aactcaaagg ccagatccgc
     3001 ggtctgttga gcaaacaaca cctcaccgaa gccgaaaaga aaaaggccga agaactgatc
     3061 aaggaactca acgacaaggt cagcaacctc gaacaacagg tagcgcagct tacccaggac
     3121 aatcaacagc tgacggctga caaaacccag ctgacagctg acaaggaaaa gctgaccaac
     3181 gacctgacaa ccaccaatac ggcaaagcag gacctcgaaa agaaagtaga cgttgcttct
     3241 acgctcaacg cctccaacat ctccgtcact ccgatgcatg aaaaaagcaa tggccaggat
     3301 aaggaaacca ccactgccaa gagagtgaac aagctggtta tcggctttga cgttgccaac
     3361 cgcatcgctc agaccggcca gaccgatgta tatgtctgca tcaccggccc cgatggcaag
     3421 cccatctccg ttcctgcact gggtagcggc acgttcacta cccgcgacga aggcgacaag
     3481 atgtacaccg caaaggttcc tgtcgatttc gaagcaggca agaccaaatc cgtacagttc
     3541 gcctggaaac agaacagcca gttccagaag ggtaactaca agatcgagat ctaccacaat
     3601 ggtttcaaga tcggcgaagg cacccgcgaa ctcaagaaag gcggtctctt ctcctagtct
     3661 catataggct ccacataaga aaaagccccg gtcaagcgcc ggggcttttc ttttataatc
     3721 atgcccgcaa ctataataat gcccgcaacg ccgccctccc cgtatgcacc accccggtgg
     3781 tgcccgcctt gcgcccctca tactgcagac tcaattccag gctgtttccc agcttcttgg
     3841 tgaggtccag gttccacaga tagttcttcc ccggctgaag cccctccagt atcgtatacg
     3901 ataccgccga ggtcgtgctg ggcgacttcc ctcccccggc cgtgtatacg atggaactca
     3961 tcgtaaactt cccctggatc gacgtgctct gcaagatatt gtacttgaaa tcggtgttcc
     4021 aggcctccga cgtatatttc tgcccgcccc agaccgggga gttctgtttg gaggtgagcc
     4081 ggcagctaag cccgatccgc aggttcgaac gccgcgtata ggtaagcccg ggctccgccg
     4141 accactgttt caggttgtaa ttggcgctgt cgaaattcgt actggaattg ttgtagtcgt
     4201 tgaccccgtt acggaatacc cccgtcagcg cgatcgcctt gttcatattc aaccgggtcc
     4261 gcagcgacca ctcattggtc tgcctcctct catacccata ggtaagcagc gtcttccccc
     4321 cattccgcgt attgctgaga tcaaaacccc agcggggatc gctccggttg aaagaaagcg
     4381 tattcaccag gatcatcgtc cgggtaataa gcgaagtatc gctgagcggc gacttgaagg
     4441 gattgtactc gataagcccc ctggcctcct g
//
LOCUS       DATGFG010000553         7536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_503183, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000553 DATGFG010000000
VERSION     DATGFG010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503183"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VKQ52_10360"
     CDS             complement(<1..369)
                     /locus_tag="VKQ52_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87636.1"
                     /translation="MKSLLFIPICAISLATAAQNLKTNDVPAVVKAALVKQFPQASKV
                     GWEKEKGNYEANWGGRSGEDHSVQFTPGGQFVEQVDAIAVKDLPSSIVSYVKNHYNTM
                     IKEAGKRLDAKGAHTYEVEVK"
     gene            554..1981
                     /gene="atpD"
                     /locus_tag="VKQ52_10365"
     CDS             554..1981
                     /gene="atpD"
                     /locus_tag="VKQ52_10365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HLZ87637.1"
                     /translation="MANTTQKSGRVVAIRGSVVDVWFPGRLPLINTLLYCGERGEIVI
                     EVQFQLDERTVRGIALTPAQGLARGVEAVNSGRPLEAPVGEGTLSHMFNVFGRPIDHS
                     VAIDGMRYRSVHGRPPALRQRSTHSEVFETGIKVIDLLMPLEKGGKAGLFGGAGVGKT
                     VLLTEMIHNMAGFHKGVSIFCGIGERCREGEELYRQMKAAGVLEHMVMVFGQMNEPPG
                     SRFRVGHTALTIAEYFRDEAHKDVLLLVDNIFRFIQAGMEVSGLMGQMPSRMGYQPTM
                     GTELSQLEERIANTASGAITSIQAVYVPADDLMDPAAVHTFSHLSASIVLSRKKASEG
                     LYPAIDPLQSNSRMMNPAVIGERHYQLAQQVRQTLAQYEELKDIIAMLGLDQLSAADR
                     AIVSRARQLERFLTQPFFTTEAFNGIKGRMVALQQCLDGCERILADAFKDYPEAALYM
                     LGAVDEVKHTDRHDSNIDQSQHPGA"
     gene            1941..2342
                     /locus_tag="VKQ52_10370"
     CDS             1941..2342
                     /locus_tag="VKQ52_10370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HLZ87638.1"
                     /translation="MIVTSINLSILVPDRVFLQEKGVRSVVAESLRGAWGLLPNRLDC
                     VLVLKPGILTYRDKDSRERFVAIDQGVLIKTGPEIRLSVRHAVEGPDLGQLRERVERE
                     FRRLGEEEAEMRSAIYKVESGFIRRLLKMRQ"
     gene            2350..2646
                     /locus_tag="VKQ52_10375"
     CDS             2350..2646
                     /locus_tag="VKQ52_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HLZ87639.1"
                     /translation="MAKDDFRRDVGTDERRKLHARKVKGHNPLTGFAVFGLIGWSVAV
                     PTLVGVAAGLALDRGHTGGRSWTLTLLVAGLVLGCWNAWYWVRKEGDKINKENK"
     gene            2649..2933
                     /locus_tag="VKQ52_10380"
     CDS             2649..2933
                     /locus_tag="VKQ52_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HLZ87640.1"
                     /translation="MAYIAGTFFAGLLGGFFFFGGLRYSVGKMLTSAYGWLWALGGFV
                     IRMVGCLALLYFSAGSDAVRWLAGLAGMMVARTLILRFTKAMEVCHAAKS"
     gene            2917..3588
                     /locus_tag="VKQ52_10385"
     CDS             2917..3588
                     /locus_tag="VKQ52_10385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLZ87641.1"
                     /translation="MRLSPDDLVYWRHGIVKLNATIVFTWAIMLVLVVGARLITRRLK
                     SDLRISGWQALLEMLVLGIGDQIREVGLREPRRYIGFLGSLFLLIATANLCTIIPGYE
                     PPTGSLSTTAALALSVFGAVPVFGIRTAGWKNYLASYVKPSFILLPFNIISEFSRTLA
                     LAVRLFGNIMSGSMILGILLIIAPLFFPVLLNVLGLLTGMVQAYIFSILAAVYIAAAS
                     NAKNE"
     gene            3598..3867
                     /locus_tag="VKQ52_10390"
     CDS             3598..3867
                     /locus_tag="VKQ52_10390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HLZ87642.1"
                     /translation="MDNMTVIGIASIVTAGATTGIGCIAPAISEGKAIVAALSSLAQQ
                     PDASSTITRTLFVGLAMIESVAIYCFVVSMILIFANPFWNHVTVK"
     gene            3874..4677
                     /locus_tag="VKQ52_10395"
     CDS             3874..4677
                     /locus_tag="VKQ52_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87643.1"
                     /translation="MSIDWFTVVAQIVNFLVLVWLLQRFLYKPVLDAVKKREQKIAGQ
                     LADAAAALSSADGKKKEYEEKRLALEQGSSALMETAKAEAGTEKDRLLAEARKVVETQ
                     RAGWLEALRQEREHAGREMVDKTRSAVLDIARHCLQDLADTGLQERMTAAFMRMIDGL
                     VDNQRQVLAAALRASPVVTVKSAFALREEDRTVLAEKIAALRGAGGEGIGFEVDPALL
                     CGIGLYMSGYKLEWAIGDYLEKIGASAAELSWQEMSATAISSGTSIKTV"
     gene            4684..5124
                     /locus_tag="VKQ52_10400"
     CDS             4684..5124
                     /locus_tag="VKQ52_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87644.1"
                     /translation="MEADIFLRSYDVALDAIARATTQVRPMATLEETGKVMAIGAGVV
                     TVSGLPGAGLEELLRFPGGLYGIAFDLDQESIGAVLLDDYRHLNTGDPVERTGRVMDV
                     PVGEGLIGRVVDPLGRPLDGGGRYCTATGCPSNEPPPRSWTGRR"
     gene            5106..6203
                     /gene="atpA"
                     /locus_tag="VKQ52_10405"
     CDS             5106..6203
                     /gene="atpA"
                     /locus_tag="VKQ52_10405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HLZ87645.1"
                     /translation="MDRAPVTVPLQTGIKVIDALIPIGRGQRELILGDRQTGKTAIAI
                     DTILNQRSAEKQVLCIYCAIRQQASGVARIIAELQEKGAMDYTTVVFTEGNDPPGLAY
                     IAPYAATSIAEYFMEAGRDVLIVCDDLTKHARAYREISLLLRRPPGREAYPGDIFYIH
                     SRLLERATRLRPEAGGGSLTALPIVETEAQNMSAYIPTNLISITDGQVYLSPTLFELG
                     VLPAVDVGRSVSRVGGAAQLPAYHRVTGDLKLGYAQFGELESFARFGTRLDEHTRRVI
                     IRGQRIRQVLKQAEHAPVVVLEQIVLLLALMEGCFDQVDTAKMPEAEGKVRGSAMVAP
                     GTLRRKLLGGEKMSDADKAVLLAAAREALTK"
     gene            6200..7204
                     /locus_tag="VKQ52_10410"
     CDS             6200..7204
                     /locus_tag="VKQ52_10410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HLZ87646.1"
                     /translation="MSDSIENLQHQIVTATELHSVVRTMKALAAAGISQYEHAVVSLQ
                     EYYRSVESGLAACIRQLGAEAPAAGWLADEMSGAGRRKSPAAVIVFGSDQGLVGQFNG
                     SLVQFCKEKIEAIASEKVILVVGERMEAEWQGGEAQPAGRYELPVSVSGIGLLVDRLL
                     IDLERQRDEGTIGDVYVFHNRPSGRAGYKPVIQRLLPLAEAWRAELSGLSWAGEKRPE
                     VIGGVVATSMALIREYLFVSLFKSCAESLAAENASRLAAMQNAEKNIGQLSDELVQAY
                     HRLRQEEIDEELSDIISGSEALSRRNRGVGLPQYASNHHQVSSAAVLLNTMRLYSLKK
                     "
     gene            7219..>7536
                     /locus_tag="VKQ52_10415"
     CDS             7219..>7536
                     /locus_tag="VKQ52_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLZ87647.1"
                     /translation="MKKVMIAVDGGVLSEATAHAGYELARWMGAVVALVYVVDNIGAM
                     GDSGISAREWEALEEKEGRDMLERLKREMGATDAWIFVEAGSAARTILKLAAEWGADV
                     LVIG"
BASE COUNT         1531 a         1738 c         2704 g         1563 t
ORIGIN      
        1 ttttacttcg acttcgtagg tgtgtgctcc cttggcatct aagcgtttgc cggcttcctt
       61 gatcatcgtg ttatagtggt ttttcacata gctgacgatc gatgacggta gatctttgac
      121 ggcgatggcg tcgacttgtt cgacgaactg gccgccgggc gtgaattgaa cagaatggtc
      181 ttcgccggaa cgaccgcccc agttggcttc atagttccct ttttccttct cccagccgac
      241 ctttgaggcc tggggaaatt gtttgacaag cgcggccttt acgacagcag gtacgtcgtt
      301 ggttttgaga ttctgcgcgg cagttgccag gctgatggcg cagatcggga tgaacaaaag
      361 tgatttcata aattagatat ttcgtataaa ggtatttggt gattctgagg aattgctgaa
      421 ttctgatact ttcggatagc tgcaaaggta tggtgtgatg gaacgtgacg acgatgacga
      481 gcatcataga gtgatgtgag ataggtcagt gggaggaaca gcggttgagg gtaattttca
      541 ttattaaata actatggcaa atacaacgca gaagtcgggt cgcgtggtcg cgatacgagg
      601 aagcgtagtg gacgtatggt tccccgggcg gcttccgttg atcaatacct tgctgtattg
      661 tggagaacgc ggggagatcg tgatcgaagt gcaatttcag ctggatgagc ggaccgtgcg
      721 cgggattgcg ctgacgccgg cgcaagggct ggcaagaggg gtagaggcgg tgaatagcgg
      781 caggccgttg gaggcgccgg tgggggaagg aacactctcc cacatgttca atgtctttgg
      841 caggcccatc gaccatagcg tagcgattga tggtatgcgc tacagatcgg tgcatggccg
      901 gccgccagcg ctccggcagc ggtcaaccca ttcggaggta ttcgaaacgg ggatcaaggt
      961 gatcgacttg ctgatgccac tggagaaggg cggcaaggcg gggctcttcg ggggagctgg
     1021 agtcggcaag acggtattgc tcacggagat gatccataat atggcgggct ttcataaggg
     1081 cgtcagtatc ttctgcggca tcggcgaaag atgtcgcgaa ggagaagagt tgtacaggca
     1141 gatgaaagcg gcgggagtgc tggagcatat ggtgatggtg ttcgggcaga tgaacgaacc
     1201 acctggcagc cggttccgcg tgggacacac ggcgctgacg atcgcggaat attttcggga
     1261 cgaggcgcat aaggatgtgt tgctgctggt ggacaatatt tttcgtttta tccaggcggg
     1321 gatggaggtg tcggggctca tggggcagat gccgtcgcgg atggggtatc agccgactat
     1381 gggcacggag ttgtcgcagt tggaggagcg catcgccaat accgcttcgg gtgcaattac
     1441 gtcgatccag gcggtctatg tgccggcgga cgaccttatg gatccggcag cagtgcacac
     1501 cttttcgcat ctgtcggcat cgatcgtgtt gtcgcgcaag aaggcgagtg aaggattgta
     1561 cccggcgatc gacccgctgc aatccaattc acggatgatg aatccggcgg tgatcgggga
     1621 gcggcactac cagctggcgc agcaggtgcg gcagacgctt gcacagtacg aagagctcaa
     1681 ggacatcatc gcgatgctgg ggctcgacca gctgtcggct gcggacaggg cgatcgtgag
     1741 cagggcgcgg cagctggaaa ggtttctcac gcagcccttc tttacgacgg aggcgttcaa
     1801 tgggataaag ggcaggatgg tcgcgctgca gcaatgtctg gacggatgcg agcggatcct
     1861 cgcggatgcc ttcaaagact acccggaagc cgcgttgtat atgctcgggg cggtggacga
     1921 agtgaaacat acggatagac atgatagtaa catcgatcaa tctcagcatc ctggtgcctg
     1981 accgggtttt cctgcaggag aagggggtgc gcagcgttgt ggcggagagc ttgagaggcg
     2041 cctggggttt gttgccgaac cggctggact gcgtgctggt gctgaagccg ggaatattga
     2101 cttaccggga caaagatagt cgggagcgat tcgtagcgat cgaccagggc gtgctgataa
     2161 aaacggggcc ggagatccgg ctgtcggtgc ggcatgcggt ggaaggcccc gaccttggcc
     2221 aattgcgcga gcgggtggag cgggagtttc ggcggctggg cgaagaggaa gcggagatgc
     2281 ggtcggcgat ttataaggtg gaaagcggct ttatccgccg gttattaaag atgcgtcaat
     2341 gacagtatta tggcgaagga cgattttcgc agggacgtgg gcaccgatga gcggcgaaag
     2401 ctgcacgcca ggaaggtgaa ggggcacaac ccgctgacgg gctttgccgt cttcgggttg
     2461 atcggctggt cggtggcggt gcccacgttg gtgggggtgg cggcaggcct ggccctggac
     2521 cgcgggcata ccggagggcg gtcgtggacg cttacgttgc tcgtcgctgg gctggtgctg
     2581 ggctgctgga atgcctggta ttgggtgagg aaggaaggcg ataaaatcaa taaggaaaac
     2641 aaataactat ggcatacatc gcgggtacat ttttcgctgg cctgctgggc ggattcttct
     2701 tctttggcgg gttacgctac agcgtcggca aaatgctgac gtcggcctat gggtggttgt
     2761 gggcgctggg cggcttcgta atccgtatgg tgggctgtct ggcgctcctg tatttttcgg
     2821 cgggaagcga cgcggtgcga tggctcgccg ggctggccgg gatgatggtt gcgcgcacgc
     2881 tgatcttgcg gttcacaaaa gcaatggagg tgtgtcatgc ggctaagtcc tgacgacctg
     2941 gtctattggc ggcatggtat cgtcaagctg aatgcgacga tcgtctttac gtgggcgatc
     3001 atgctcgtgc ttgtcgtggg cgcgcggctg atcacgcgga ggctgaagtc ggacctgcgg
     3061 atctccggct ggcaggcatt gctggaaatg ctggtgctgg ggatcgggga ccagatcagg
     3121 gaggtggggt tgcgggagcc acgcaggtat atcgggtttc tcggttcctt gttcctgctg
     3181 atcgcgacgg ccaacctttg cacgatcatt ccggggtatg aaccaccgac agggagcctg
     3241 tcgacgacgg cggcgctggc gctgtcggtg tttggggcgg tgccggtgtt cggcatccgt
     3301 acagcgggat ggaagaacta cctggcctcg tatgtaaagc cgagcttcat cctgctaccg
     3361 ttcaacatca tcagcgaatt ttcgcgaacg ctggccctgg cggtgcggct ctttgggaat
     3421 atcatgagcg gcagcatgat cctgggaata ctactgatca tcgcgccgct gttcttcccg
     3481 gtgttgttga atgtgctggg cttgctcacg ggtatggtgc aggcatatat cttcagcata
     3541 ttggccgcgg tctatattgc cgcggcgtcg aatgctaaaa acgaataaac aattgatatg
     3601 gacaacatga ctgttatcgg cattgcctcg atcgttactg cgggagcgac gacggggata
     3661 gggtgtatcg ctccggcgat atcggagggc aaggccatcg tggcggcgct gtcgtcgctg
     3721 gcacagcagc cggacgcttc ttctacgatc acgcggacct tgttcgtcgg cctggccatg
     3781 atcgaatcgg tggcgatcta ttgcttcgtg gtatcgatga tcctgatctt cgccaacccc
     3841 ttctggaatc acgttacggt aaaataaaac ggtatgtcga tcgattggtt cacggtagtg
     3901 gcgcaaatcg tcaatttcct ggtactggtg tggttgctcc agcgtttcct ctacaagcct
     3961 gtgctcgacg cagtgaagaa gcgcgagcag aagatcgcag gccagctcgc ggatgccgct
     4021 gctgctttga gttccgccga tgggaagaag aaggagtatg aagagaagcg attggcgttg
     4081 gaacagggca gctcggcttt aatggagacg gcaaaggcgg aggcgggaac ggaaaaagac
     4141 cggctgttgg cggaggcgcg caaggtggtg gaaacgcaac gggcaggctg gctggaggcg
     4201 ttgcgacagg aacgggagca cgcgggccgg gaaatggtgg acaagacgcg atcggcggtg
     4261 ctggacattg cgaggcactg cctgcaagac ctggcggata ccgggttgca ggagcggatg
     4321 acggcagcat ttatgcggat gatcgacggg ttggtggaca atcaacggca agtgctcgcg
     4381 gcggccttaa gagcttcgcc ggtagtaacg gtgaagagcg cgtttgcgct gagggaggag
     4441 gaccggactg tgttagcgga aaagatagcg gcgcttcgcg gcgcgggcgg agaaggaatc
     4501 ggcttcgagg tcgatccggc tttgctctgc ggtatagggc tctatatgtc cggctataag
     4561 ctggaatggg cgatcggcga ctacctggag aagatcgggg cgtcggcggc cgagctcagc
     4621 tggcaggaga tgtctgcgac ggctatatca tcggggactt cgataaaaac ggtataatat
     4681 aatatggaag cggacatctt cttacgatcc tatgatgtgg cgttggacgc cattgcgagg
     4741 gccaccacgc aggtgcgccc catggcgacg ctggaagaga cgggtaaggt aatggccatc
     4801 ggggccggtg tggtgaccgt gtcgggattg ccgggcgcgg ggctggagga gttgctgcgg
     4861 ttcccgggtg gtttgtacgg cattgccttc gacctggacc aggagtccat cggggcggtc
     4921 ctgttggacg attaccggca cctcaacacg ggtgatccag tggagcgcac gggcagggtg
     4981 atggacgtgc cggtgggcga agggctcata gggcgggtgg tcgatccgct gggccgtccg
     5041 ctggacggcg ggggccggta ttgtacagcc acaggctgcc catcgaacga accgcccccg
     5101 cgatcatgga ccgggcgccg gtgaccgtac cgcttcagac ggggatcaag gtgatcgacg
     5161 cgttgattcc catcggccgg ggtcagcggg aattgatcct gggggacagg caaacgggga
     5221 aaacggccat cgcgatcgat acgatcctga accagcgcag tgcggagaag caggtgctgt
     5281 gtatctattg tgcgatacgg cagcaagcgt caggcgtggc gaggatcatc gcggagctac
     5341 aggaaaaagg ggccatggac tatacgacgg tagtctttac tgaaggtaac gacccgccag
     5401 gactggctta tatcgcgcca tatgcggcga cgagcatagc ggaatatttc atggaggcgg
     5461 gccgggatgt actgatcgtg tgtgacgacc tcaccaagca tgcgagggct taccgggaaa
     5521 tttcgttgtt gctgcggcgg ccgccgggac gggaagcgta tccgggggat atcttttaca
     5581 tccattcccg gttgctggag cgggccacgc ggctgcggcc ggaagcgggg ggaggatcat
     5641 tgacggcctt gcccatcgtg gagacggaag cgcagaatat gtcggcatac attcctacca
     5701 acctgatctc gatcaccgac gggcaggtat acctgtcgcc gacgcttttc gaactgggcg
     5761 tgctgccggc ggtggacgtc ggcaggtcgg tttcgagggt gggcggcgcc gcccaactac
     5821 cggcctatca ccgggtgaca ggcgatctca aactggggta tgcccagttc ggggagttgg
     5881 agagctttgc gcgcttcggc acccggctgg acgaacatac ccggcgggtg atcatccggg
     5941 ggcaacgcat ccggcaggtt ctaaaacagg cggagcatgc gccggtagta gtgttggagc
     6001 agattgtgct gctgctggcg ttgatggaag ggtgttttga ccaggtcgat acggcaaaga
     6061 tgccggaggc ggaaggcaag gtgcgcgggt cagcgatggt ggcgccggga acgctgaggc
     6121 gcaaattgct ggggggagag aaaatgagcg atgcggacaa agcggttttg ctggccgcgg
     6181 cgagggaggc gctgacgaaa tgagtgacag tattgaaaat ctgcaacacc agatcgttac
     6241 ggcaactgag ctgcactcgg tagtacggac gatgaaggcg ctggcagcgg ccggtatcag
     6301 ccagtatgag catgcggtcg tgtcgctgca ggaatactac cggtcggtgg aatcggggct
     6361 ggcggcgtgt atccggcagt tgggcgcaga ggcgccggcg gcagggtggt tggcggatga
     6421 aatgtcgggt gctggacgtc gaaagtctcc ggcggcggtg atcgtgttcg gatcggacca
     6481 ggggttggtg ggacaattca acgggtcact cgtgcaattc tgcaaagaaa agatcgaggc
     6541 catcgcttcg gaaaaggtca tcctggtggt gggcgagcgt atggaagcgg aatggcaggg
     6601 cggtgaggcg cagccggcgg gccggtacga actgccggta tcggtgagcg gaataggatt
     6661 gttggtcgac cggttgttga tcgacctgga acggcagcga gacgagggaa cgattggaga
     6721 cgtgtatgtt ttccacaacc gcccgtcggg gagggccggt tataagccgg tgatccagcg
     6781 gctattgccc ctggcggagg cctggcgcgc ggaattgtcc ggcctgtcat gggcggggga
     6841 aaaacggcct gaggtgatcg gcggcgtggt cgcgacaagc atggcgctga tccgggaata
     6901 cctttttgtt tcgttgttta agtcctgtgc ggaatcattg gcggcggaga atgcgagccg
     6961 cctggcggct atgcagaacg cggagaagaa catcgggcag ttgtcggacg aactggtgca
     7021 ggcatatcac cggttgcggc aggaagagat cgacgaggag ctttcggata ttatttcggg
     7081 ctcggaggcg ctgtcacggc ggaatcgggg tgttggcttg cctcaatacg caagcaacca
     7141 ccaccaggtg agcagcgcag cggtgttgtt gaacacgatg cggttgtatt cgttgaaaaa
     7201 ataaacagga tggcatatat gaaaaaagta atgattgcgg ttgacggagg cgttctttcc
     7261 gaagccacgg cgcatgccgg gtatgagctc gcccgatgga tgggcgcggt tgtggcgctg
     7321 gtatacgtag tggataatat cggcgctatg ggcgatagcg ggatatcggc cagggaatgg
     7381 gaggcgttgg aggaaaagga ggggcgcgat atgctggaaa ggctcaaaag ggagatgggg
     7441 gctacggacg cctggatatt cgtcgaggca ggttccgcgg cgcggacgat tctgaagctt
     7501 gctgcggaat ggggcgcaga tgtgctggta atcggt
//
LOCUS       DATGFG010000554         5398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_172303, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000554 DATGFG010000000
VERSION     DATGFG010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172303"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VKQ52_10420"
     CDS             <1..666
                     /locus_tag="VKQ52_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLZ87648.1"
                     /translation="CLKALQQAGKKIIFVDCSGSAIGLVPETATCNGILQAWYPGEKG
                     GEAVADVLWGDYNPSGKLPVTFYKNVDQIPGFEDYTMKGRTYRFMSDPLFPFGFGLSY
                     TTFSIGEAHLSAKEMQRDGSVDLTVPVSNIGRRQGTEVLQVYVRKAGDTDGPLKTLRN
                     FRRVKLTPGGRSEVTMNLNADVFQFFDRGSGKMMTTPGEYELLYGTSSADNDLKSIKV
                     TIL"
     gene            663..>5398
                     /locus_tag="VKQ52_10425"
     CDS             663..>5398
                     /locus_tag="VKQ52_10425"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HLZ87649.1"
                     /translation="MSARVILCLLLLAGYAAGAQQLRVESPNHRIIAALYCVQGGDRG
                     EWYLELAGMVPRISLGLERSDQGFRNDLRFIRAGRPVPIDEQYTVVHGKRSHCNNRAN
                     EVTVSFENAELSKMELIIRAYDDGLAFRYRFPEGRDSLVVKEEMTAYTIAPGTARWLE
                     KWNPANEGLYKAMKDDSVRQDWCYPALLNVPGSDNWFLIHEAGLDRTYCGSKLSNIAA
                     GDTYKITFPDQKDGRGLGASAPSIRLPWQSPWRVIIAGSLNDIVRSTLVEDVSPPSIL
                     PKTDWIKPGLVSWNYWSHNHGTKDYTVVCAFTDLAARMNWPYTLFDWEWDAMGNGGAL
                     EDAARYALSKGVRPLIWYNSGGPHTYVTATPRDRMLTHDNRVEEFRKLKQLGIAGVKV
                     DFFESEKQDMIRYYLDILEDAAKAEMMVYFHGSLVPRGWSRTYPNLMTCEAVRGAEWY
                     NNGPDFTMTAPGHNCTLPFTRNVVGPMDYTPVTFTNSQFPHVTSYGHELALSVVFESG
                     LQHLADRPEGYDGLPDAARHFLMEVPNAWDDSKLLEGYPGQEIILARRKGDGWWLGGL
                     NATLREKTKTLKFGFLPAGMKYKLTLIADGEHDKALATSYQVVDSSSEVEVKMLRRGG
                     FAASLVPVASAAYARILPGKGTFVNPVLPGDHPDPTLLKVGDDFYHCGSSFQFNPYLP
                     IYHSRDLVHWEVIARVVPPSLAGFVDDHPSAGIWQGAITRFYGSYWIYFSAGGQWFCK
                     ADNPAGPWSAPERVKSPLNVGYDNSIFVDDDGSPYMIMKNGPKFNRIQEIGRDGQLTG
                     KMLNLDWINASGQYSWAEGPVMCKRNGLYYYFMAGSVAGGQYVLKATALTEDSTKWTR
                     LGDFFRPATDRENRFPVVNHMSAPVRLADGSWWTIAQSYERLPGDDWSGMGRQTGLYR
                     VTWDGDRPWGEAPVSTPLTAPHLPRAGLLRTSAQSDAFDSGSLGLCWHFLNRGAAANI
                     SLTARKGWARLSPEGGRTHLVQKETDHYYSAVTCVDMDATDTAARAGIYLTNGNESMV
                     VRLYSGYDGERKIILVMDTALRSAANPFGRTVWLKLRRDAHMLTGYFSGDGKDWKPLG
                     PPISAVRLDKEQPKWNWWVGTSVGLFAEGRPADFDAFVIKDGFSALPAIGHSDYAQLS
                     TNVADDYVAASATAGGWFLLTGVDIGQATAGIEAMVSATEPGKLEIWLDELGMAKPAA
                     VIPVEVSGRAGAWKTFSTKLKGISGRHDVYVLISGGTPGAIRVRSLRFTRCDGQSKRV
                     DTASFPWPVHADNGDGTFTNPVIMADFPDVDVIRVKDTYYMLATTMFTFPGVPLLQSH
                     DLVNWSYCTNIVGRMDEGPCYDLDGCNRYGHGQWAGSLRYHDGVFYVLFNTLNEGAFL
                     CTATEPAGAWKIRRLGRGFHDCGLLFDDGGKIYVASGYNQIHITELDSNFSPVSGDSL
                     VFTGDLRRGLEGTHVYKLNGYYYLYCTYGGGDGFQVALRSKNIWGPYQEKLVLHETDR
                     SSVNFGIHQGALIQTQTGEWWTMLFIDRGPFGRFPSLQPVSWQDNWPVAGVEGRAVAK
                     FKKPDVGREYPVKNLPTSDEFTADTLG"
BASE COUNT         1122 a         1372 c         1750 g         1154 t
ORIGIN      
        1 tgcctcaagg ccttgcagca agcgggcaag aagatcattt ttgtcgattg ttccggttcc
       61 gctatcgggc tggtgcctga gaccgcaacc tgtaatggga tcctgcaggc atggtatccc
      121 ggcgaaaagg gcggtgaagc cgtggccgat gtactctggg gggattataa cccttcgggc
      181 aagctgccgg tcacctttta taagaacgtt gaccagattc cgggattcga ggattatacc
      241 atgaagggaa ggacctaccg gtttatgtcg gatccgctgt tccccttcgg gtttggcctc
      301 agctatacga ccttttcgat aggggaagca catctctcgg caaaggagat gcagcgggat
      361 ggctcggtcg atctgacggt acccgtgtcg aatataggac gccgtcaggg tacggaggta
      421 ttgcaggtgt atgtacgcaa ggcgggcgat accgatggtc ctttaaaaac gttaagaaac
      481 ttccgtcggg tgaaacttac gccgggcggg cgcagcgagg tgacgatgaa cctgaatgcc
      541 gatgtgttcc agttcttcga ccgtggcagc ggtaaaatga tgacgacccc gggtgaatac
      601 gagctgctgt acggcaccag ctcggcggat aatgacctca aatccataaa agtaacgatc
      661 ttatgagcgc ccgggtaatt ctatgtcttc tgttgcttgc gggctacgct gccggcgcgc
      721 agcagttgcg cgtcgagtcg ccgaaccacc gcatcatcgc ggcactctat tgcgtgcagg
      781 gaggcgatcg tggcgaatgg tacctggagc tggcggggat ggtccccagg atctcgctgg
      841 ggctggagcg gagcgaccag ggattccgga acgaccttcg attcataaga gcaggaagac
      901 ccgtgcccat cgacgagcag tatactgtcg tgcacggcaa gcggtctcat tgcaacaaca
      961 gggccaacga ggtgacggta tcgttcgaga acgcggagct gtcgaaaatg gagttgatca
     1021 tacgagctta tgacgacggc ctggccttcc gttaccggtt cccggagggt agggattccc
     1081 tcgtcgtaaa ggaagagatg acggcttata ccattgcgcc ggggactgcc cgatggctgg
     1141 agaaatggaa cccggccaat gaagggttat acaaagcgat gaaggacgac agcgtccgcc
     1201 aggactggtg ttacccggcc ctgctcaatg tgccggggag cgataattgg ttccttatcc
     1261 acgaggctgg tctggatagg acctattgcg gctcgaagct gagcaatatc gccgcgggcg
     1321 acacctataa aatcactttt cccgatcaga aggatggcag gggactgggt gcttccgcgc
     1381 cttccatccg gctgccctgg caatcgccct ggcgagtgat cattgccggg agtctcaacg
     1441 atatcgtcag gtcgaccctg gtggaggatg tatcgcctcc ttccatactg ccgaagacgg
     1501 attggataaa gcctggtctt gtttcctgga attattggtc gcataatcat ggcaccaaag
     1561 actataccgt tgtctgcgca ttcaccgatc ttgccgcgag gatgaactgg ccgtatacgc
     1621 tcttcgattg ggagtgggat gccatgggaa atggcggcgc gctggaggat gcggccagat
     1681 atgcactctc gaagggggta cggccgctga tctggtataa ttcgggcgga ccacatacct
     1741 acgtgaccgc cacgccgcgg gaccggatgc tgacgcatga caaccgcgtg gaggagtttc
     1801 ggaagctgaa gcagctgggt attgcggggg tcaaggtgga tttcttcgaa agcgagaagc
     1861 aggatatgat ccggtattat cttgatattc tcgaagatgc cgcgaaggcg gagatgatgg
     1921 tttatttcca tggtagtctt gttcccaggg gatggtcgcg cacctatcct aacctgatga
     1981 cctgcgaggc ggtgcgcggg gcggaatggt ataataatgg ccccgatttt acgatgacgg
     2041 cgcccgggca caattgtacg ctgccgttca cacggaacgt cgtgggaccg atggattaca
     2101 cacccgtgac ctttaccaat tcccagtttc cgcatgtgac atcctatggc catgagctgg
     2161 cgctgagtgt agtgttcgag tcgggactgc agcacctggc ggaccggccc gaagggtacg
     2221 acgggctgcc ggatgcggcc aggcatttcc tgatggaggt tcccaatgcc tgggacgata
     2281 gtaagctcct ggagggctat ccggggcagg agatcatcct ggcacgccgc aaaggcgatg
     2341 gttggtggct gggcggtctg aatgccacgc tgcgggaaaa gacgaaaacg ctgaaattcg
     2401 gctttctgcc cgccgggatg aaatacaagc tgacgctgat cgcggatggg gagcacgata
     2461 aagcgctggc gacgagctac caggtggtgg acagttcgag cgaggtggag gtgaagatgc
     2521 ttcgccgggg cggttttgcg gcttcgctcg tgcctgtggc gtctgctgcg tatgcccgga
     2581 tcctacccgg caaaggaacg tttgtcaacc cggtattgcc gggagatcat cctgacccga
     2641 ccctgctgaa ggtaggagac gatttctatc attgcggatc gagtttccag ttcaatccct
     2701 atctcccgat ctaccacagc cgggacctgg tgcactggga agtgatcgca agggtggtgc
     2761 cgccatcact tgccgggttc gtggacgacc acccatcggc cggcatctgg cagggtgcga
     2821 tcacccggtt ctacgggtcc tactggattt atttttccgc cggcggccaa tggttctgca
     2881 aggcggacaa tcccgcgggc ccgtggtcgg cgccggaacg ggtaaagagc ccgctgaacg
     2941 tcggatatga caattcgatc ttcgtcgatg atgacgggtc accatacatg atcatgaaga
     3001 acggtccgaa gttcaaccgc atccaggaga tcggccggga cggccagttg acgggcaaga
     3061 tgctgaacct ggactggatc aatgcgagcg gacagtatag ttgggcggaa ggaccggtga
     3121 tgtgcaagcg gaatggcctc tactattatt tcatggccgg cagtgtagcc ggaggacaat
     3181 atgtgctgaa ggcgactgcg ttgacggaag attcgacgaa gtggacccgg ctgggcgatt
     3241 ttttccggcc tgccacggat cgtgagaacc gttttcccgt cgtcaaccat atgtccgctc
     3301 ctgtccggct ggcggatggc agctggtgga cgatcgcaca gagctatgaa cgcctgccag
     3361 gggacgactg gtcgggcatg ggccggcaga ctggtctgta ccgtgtgacc tgggatggtg
     3421 accggccctg gggcgaggcg ccggtcagca cgccgttgac ggctccacac ctgccgcgcg
     3481 ctgggctctt acgaacaagc gcgcaatcgg acgcgttcga ctccggttcg ctgggccttt
     3541 gctggcattt tcttaatcgc ggggcagcgg ctaatatatc gctaactgcc cggaaggggt
     3601 gggcaagact gagtcccgag ggcggcagga cacaccttgt acagaaagag acggatcatt
     3661 attattcggc cgtgacctgc gtggacatgg atgcgaccga tacggcggcc agagcgggca
     3721 tatatctcac gaacggcaat gagtcgatgg ttgttcgtct ctacagtggt tatgacgggg
     3781 agaggaaaat tatactggtt atggatacgg ctctccggtc ggcagccaat ccattcgggc
     3841 gcacggtatg gctgaagctg aggcgcgatg cgcacatgct cacaggttat tttagcggcg
     3901 atggcaagga ctggaagccg ctggggccgc ccatcagcgc ggtacggttg gacaaggaac
     3961 agccgaaatg gaactggtgg gtgggtacga gcgtgggcct ctttgcggaa ggcaggcccg
     4021 cggatttcga tgcattcgtt atcaaggatg gtttttctgc cttgccggcg atcggccata
     4081 gtgattatgc gcagctgtcg acgaatgtcg cggacgatta tgtcgccgcg tcggcgacgg
     4141 cgggcggctg gttcctgctg accggcgtcg atatcgggca ggcaaccgcc gggatagagg
     4201 cgatggtatc ggccacagaa cctggtaagc tggagatctg gctggacgag ttgggcatgg
     4261 ctaaacccgc cgcagtgatc ccggtggagg ttagcggacg ggccggagcg tggaagacat
     4321 ttagtacaaa gctgaaagga atttccggcc ggcacgacgt gtatgtacta atatccggag
     4381 gcacgccggg tgcgatccgt gtaagatcgc tccgttttac ccggtgtgac ggccagtcca
     4441 agcgggtgga tacggcaagc tttccctggc ccgtgcatgc cgataatggc gatggcacct
     4501 ttaccaatcc tgtgatcatg gcggatttcc ccgatgtgga tgtgatccgg gtaaaggaca
     4561 cctattatat gctggcgacc accatgttca cctttcccgg tgtcccgctg ttgcaatcgc
     4621 atgaccttgt gaactggagc tattgcacca atatcgtggg ccgcatggat gagggcccct
     4681 gctatgacct ggacggctgt aatcgttatg ggcacggtca atgggccggc agcctgcggt
     4741 atcatgacgg tgttttttat gtcctgttca atacgttgaa cgaaggggcc tttttgtgta
     4801 cggctaccga gccggccggc gcctggaaga tcaggcggct cggtcgggga ttccacgact
     4861 gcgggctgct gttcgacgat ggcgggaaga tctacgtggc ctccggctac aatcagatcc
     4921 atataacgga gctggatagt aatttttcgc ccgtgagcgg cgattccctg gtctttacgg
     4981 gggacctgcg gaggggtttg gaaggaaccc atgtatataa gctcaatggt tactattacc
     5041 tgtactgtac ctatggcggc ggcgatgggt tccaggtggc cttgcggtcc aaaaatatct
     5101 gggggcccta ccaggaaaaa ctggtgctcc acgagactga tcgaagcagt gtcaatttcg
     5161 ggatccatca gggagctttg atccaaacgc aaaccggtga gtggtggacc atgctgttca
     5221 tcgatagggg gccctttgga aggttcccct cactgcagcc ggtgtcgtgg caggataatt
     5281 ggccggtggc cggcgttgaa ggcagggccg tggcgaagtt caaaaagcct gatgtaggaa
     5341 gagaatatcc ggtcaagaac ctgcccactt ccgatgagtt cacggccgat acgctggg
//
LOCUS       DATGFG010000555         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1576842, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000555 DATGFG010000000
VERSION     DATGFG010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576842"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VKQ52_10430"
     CDS             <1..932
                     /locus_tag="VKQ52_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ87650.1"
                     /translation="LSSARAGTSLQAAEIARLVKLAYLQLQVYREKQLLLEQMDSIYS
                     RFSAMARLRFEKGESNLLEKTTLENQAQQIVLQLRQTLADRRNASIQLGLVLNTDQVL
                     DAADRLALPGPGAGAVSDSSVLNRHPVVGYYRQQEAIAAATTRVQRSRLLPDITIGYT
                     NQSIAGWQLTKDRSEAYYGNASRFSTVQLGLSIPIFNRAQQARIKAAEQQQAIVGATR
                     QLAVTQLQAQLSQTLNAYAKYAESVAYYRSSGLAQSNTLLQTGILSYKNGAIGYIEWG
                     ALAGSAVQIRLQYIDAISNLVTQQIELDYLFTQ"
     gene            945..2096
                     /locus_tag="VKQ52_10435"
     CDS             945..2096
                     /locus_tag="VKQ52_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ87651.1"
                     /translation="MNNIKSLKTIAFGILFTGLSCSQPSHPTKKPAGLSSPDVVVLNN
                     VQMTNAGVETAYPQQKEMQTTIRVNGTVDVPPQNLVSVSFPMGGYLRSTPLLPGYPVH
                     KGDAIAVMEDQSFVQLQQEYLTTRARLDYLSVDLQRQKELSDADATSKKTYQLALSDY
                     KTQEAVLRSTREKLRLIHIDPEKLSVGNISSQTRIYSPIDGYVSKVNVNVGKYVNPAD
                     VLFELVNPTDIHAAMTVYEKDIALFRKGMHGKVALLDQPAQSYDVETILITRNVNDGR
                     SGVIHCHFEGAHHELLPGMYLSGTFQLDSRKVVAVPEDAVVSYMGRRYVFVARKPTEF
                     QLTAVEAGGAEQGLIELTSGGNPDWGNTRIVTKGAYALLSKLKNKMQED"
BASE COUNT          590 a          671 c          591 g          484 t
ORIGIN      
        1 aactgagcag cgcaagggcg gggacttcat tgcaggccgc ggagatcgcc cgcctggtga
       61 agctggccta tctgcaactg caggtttacc gggagaaaca actcctgctg gagcaaatgg
      121 acagcatcta cagccgtttc agtgccatgg cccggcttcg gttcgaaaaa ggagaatcca
      181 atctgctgga aaaaacaacg ctggagaacc aggctcaaca gatcgtcttg cagctccgcc
      241 aaacccttgc cgacaggcgg aatgcctcca tccaattggg tctggtgctg aatacggacc
      301 aagtcctcga tgccgccgac cggcttgccc tgcctggccc cggcgccggc gcggtttctg
      361 acagcagcgt cctgaacagg caccctgtag taggatatta ccgccaacag gaagccatcg
      421 ccgccgcgac gacgcgcgtc cagcgatccc gcctccttcc cgatatcacg atcggctaca
      481 caaatcagtc tatcgccggc tggcagttga ccaaagaccg ttcggaagcc tactacggaa
      541 atgccagccg cttctcgacc gttcagctgg gactcagcat cccgatcttc aaccgcgccc
      601 aacaagcccg cataaaagcg gccgaacagc agcaggccat tgtgggggcc acccgtcaac
      661 ttgccgttac tcaattgcag gcccaattgt cgcagaccct gaatgcctac gccaaatacg
      721 cggagtccgt cgcgtattac cgcagcagtg gactcgccca gtccaatacg ctgctacaaa
      781 cgggcattct gagctacaag aacggcgcca tcggctatat cgaatgggga gcactggccg
      841 gcagcgccgt ccagatccgc ctgcaataca tcgatgcgat cagtaacctg gtaactcagc
      901 aaatcgaact cgactacctt tttacacaat aaaaccgaaa aattatgaat aatataaaat
      961 ccctgaaaac aatcgccttt ggcattctct tcacgggcct ctcgtgttcc caaccttccc
     1021 atcccacaaa gaaaccagct ggactatctt cacccgacgt cgttgtcctt aacaatgtcc
     1081 agatgaccaa cgcgggagtg gagacggcct acccgcagca aaaggaaatg caaacgacca
     1141 tccgcgtcaa tggaacggtc gacgtgccgc cgcaaaacct ggtatcggtt agctttccca
     1201 tggggggcta tctgcggtca accccgctat tgcccggtta cccggtgcat aaaggggacg
     1261 ccatcgctgt catggaagat cagtccttcg tgcagctgca gcaggaatat cttacgacca
     1321 gggcccggct ggattatctg tctgtcgatc tgcaacggca aaaggaactc agtgacgccg
     1381 acgccacgag caaaaagacc tatcagctgg cattgagcga ctataagacg caggaagcgg
     1441 ttcttcgttc aacgcgggaa aagcttcgct taatccacat cgatccggag aaactgtccg
     1501 tgggaaacat ctcgtcgcag acccgcatct attcccctat cgatggatat gtcagcaaag
     1561 tgaacgttaa cgtcggcaaa tacgtcaacc ctgccgacgt actgttcgaa ctcgtcaatc
     1621 ccacggatat tcacgcggcc atgacggtgt acgaaaagga cattgcgctg tttcgtaaag
     1681 gcatgcacgg taaagtcgcc ctgctggatc agccggcaca gtcttacgac gtggaaacca
     1741 ttttgatcac ccggaacgtc aatgacggcc gctcgggagt gatccattgc catttcgaag
     1801 gcgcccatca cgagttactg cccggaatgt acctttccgg caccttccag ctcgatagcc
     1861 gcaaggtggt ggccgttccg gaggatgccg ttgtcagtta catgggccgg agatacgtct
     1921 ttgtagcccg aaaaccaacc gaatttcagc tcacggcggt ggaggcaggc ggcgctgaac
     1981 agggcctcat cgagttgacg tcaggaggca acccggactg gggcaatacc cgtattgtta
     2041 caaaaggagc ttatgccttg ctgagtaagc tgaagaataa gatgcaggag gactagcagc
     2101 gcgaggtaga ggaagattaa gtgaagccaa aataagaagg atggcaggca ctaaacctgt
     2161 catccttttc ttcatttaat ttatagtatt caaatctacc aggattggcg catcataaaa
     2221 attcgccatt ttcattgagt gtcaccctta tgcgttggtc tccattttcc aacaatactt
     2281 tgtacttttt cgtggctttc cccgatctgt caaagtattt gacaaggtaa atgttc
//
LOCUS       DATGFG010000556         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1538203, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000556 DATGFG010000000
VERSION     DATGFG010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538203"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VKQ52_10440"
     CDS             <1..196
                     /locus_tag="VKQ52_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87652.1"
                     /translation="LKGAKRLEEAAGGKKGGKKKAAAGEAPKGKKKADVEEAEALTIY
                     LPDVIQGIRLELSKDNIPFK"
     gene            203..502
                     /locus_tag="VKQ52_10445"
     CDS             203..502
                     /locus_tag="VKQ52_10445"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HLZ87653.1"
                     /translation="MGQPAARLTDMHLCPMVTPGVPPIPHVGGPITGPGCPTVLIGGT
                     PASVMGDLCTCVGPPDSVLVASTSVLIGGKPAVRMGDSTVHGGTILGGDPTVLIG"
     gene            617..1492
                     /locus_tag="VKQ52_10450"
     CDS             617..1492
                     /locus_tag="VKQ52_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLZ87654.1"
                     /translation="MPVISIAIQKGGSGKTTTALNLGAALSREGKKVLLIDADPQANL
                     SQSLGIEDEPQFNLYSELKKEILEEGGQLSRAIVHACQGIDVIPASIDLGIVELEMAG
                     LMGREYLMSDLLKPLVDNYEYIFIDCPHAISLLTVNALVASDYVMLPLPGEFLPLKGV
                     YGFMRQFEVIRKKLNPRLELMGMVMTKYDERKQMNVGVRKQLEEKFDGKVFRTVIRTN
                     IQLAKAQEAGKDIFSYDRSANGAKDYRQLAGELMGKSYPATGVVGVAATTGIVGVAAV
                     SATAGLVGVATAAAV"
     gene            complement(1489..3873)
                     /locus_tag="VKQ52_10455"
     CDS             complement(1489..3873)
                     /locus_tag="VKQ52_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ87655.1"
                     /translation="MKKLTSLLLFLLASMIATAQNTQTIRGVVIDKTSEKPLPGVTVL
                     VVGTNLGAVTDSIGRYVLRGVPLGRQQVSYGSAGYQGASIPEVLVTAGKEVILDMALE
                     QHLVTLKTFTITAAAAKKGAATNEFSAGSARSISIDDITRYAGGRNDPSKLVSNYAGV
                     VANSDARNDIVVRGNSPAGVLWRIEGSPSPSPNHFATLGTTGGPVSALNTNMLKTSDF
                     LTGAFPAEYGNATAAVFDINLRSGNSSRHETTLQVNAFSGLEATVEGPLNNKNNGAAY
                     LASYRYALGQLTNAIGINIGTKAVPHYQDWAFNITTGNGALGRFSFFGMGGLSHIDLI
                     GSKTDSTDFYGQADEDSYDKSNFSYFGIKHTLDIDNRTYLRTVVTYAHTLDHYDQYRY
                     PDPVPPYQDRWLEYTSDNRTSTVRFSTYLNQKVDSRFSYRIGIAGEDLGLKIHVFDKT
                     ARPASAPFDTVSNYDGHPLLLQYFGQFKYRVTEQFSIVGGLHGMHFDANNSSALEPRL
                     SATYQLPAGQSISLSYGLHSQLQPLPVYYQTFDETDNIRDSSNRKLDLTRAQHFVAGY
                     EWRFLPGWRVKAEAYYQYLFNVPVERTPSGFSMLNAGADFGFPDKVGLVNKGTGTNKG
                     VELTLERFLNNGYYLLVTASVFDSKYKGSDGIQRNTAFNYKYVFNALAGKEWKLGNSG
                     ANAFTVDIRLSTIGGRYATPVNVGASLAAGYEILDTLHYNSQRLSDYFRLDTKFGYRT
                     SSRKRRLSSTLYLDLQNVTNRRNIFLLQYNQAKGSASPIYQIRFFPDILYRIQF"
     gene            4008..>4558
                     /locus_tag="VKQ52_10460"
     CDS             4008..>4558
                     /locus_tag="VKQ52_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87656.1"
                     /translation="MRWRNDKTGFDDRWVISIGIPIFSLVIPIIFFGWRIGMPPYLAW
                     NVYWPALVITATVWIGSRYIMIWARTRYPQFREVKKRLWVQMSVILLFVLFVNNVLGA
                     LLDCDAGLRDSAHQLTQADILIDSNAATILCTLLVTAIYESIYFVSELRKSIEEKELL
                     KRESLQAQVNALKIQVNPHFLFNT"
BASE COUNT          960 a         1088 c         1493 g         1017 t
ORIGIN      
        1 tctgaaaggg gccaagcggt tggaggaagc agccgggggg aagaaggggg ggaagaagaa
       61 ggcagccgct ggagaggcgc cgaagggaaa gaagaaggcc gacgtcgaag aggcggaggc
      121 gctgacgatc tatctgcccg atgtgatcca ggggatacgg ctggagttga gcaaggacaa
      181 tataccattt aaatagttgt ttatgggaca gcccgcagca agactaaccg atatgcatct
      241 atgcccgatg gtgacaccgg gtgttcctcc tattccgcat gtcggcggcc cgatcacggg
      301 tcccggttgt ccgacggtgc ttatcggcgg aacgccggca tcggtaatgg gggatctctg
      361 tacttgtgtc ggtccgccgg acagcgtttt ggtggcctct acaagtgtac tgatcggtgg
      421 gaagccggcg gtgcgaatgg gtgattcgac ggtgcacggg ggaacgatcc tgggtggtga
      481 tccgacggtg ttgatcgggt gatggtttgg ctgcagccgc gattaatcca caccgatggg
      541 gaaaaactat ttctgagtaa cggcaaacaa taaatatttt taggtacttc atcaaacccc
      601 taaaaaatag ccaaacatgc cagttatttc gattgcgatt caaaaaggcg ggtctggaaa
      661 gaccacgact gctctcaacc tgggcgcagc cttatcacgg gaaggtaaaa aagtgttact
      721 gatagacgcc gatccccagg caaatctctc tcagtcgctt ggcatcgaag acgagccgca
      781 attcaacctt tattcggaat taaagaagga gatcctggaa gaagggggtc agctgtcccg
      841 ggccattgtt catgcctgtc aggggatcga tgtgatcccg gcgtcgatcg atctgggcat
      901 cgtagagttg gagatggcgg gtctcatggg aagggaatat ctgatgagtg acctgctgaa
      961 gcccctggtc gataactatg agtacatttt catcgactgt cctcatgcga tcagcctgct
     1021 tacggtgaat gcgttggtgg ccagcgacta cgtcatgctt cctcttccgg gtgagttcct
     1081 gccgctgaag ggggtatacg ggtttatgcg gcaattcgag gtgattcgca agaaactcaa
     1141 tcccaggctg gagctgatgg gcatggtgat gaccaagtat gacgagcgta agcaaatgaa
     1201 cgtcggcgtg aggaagcagt tggaagagaa gttcgatggc aaggtattcc ggacagtgat
     1261 ccgtacgaac atccagttgg ccaaagcgca ggaagccgga aaggacatct ttagctacga
     1321 ccgcagcgca aacggcgcca aagactaccg gcagctggcg ggtgagttga tgggaaagtc
     1381 gtatcccgcc acaggcgtcg taggggtcgc cgccaccacg ggcatcgtag gggtcgccgc
     1441 cgtctcggcc accgcgggcc tggtaggagt cgccaccgca gcagctgttt aaaactggat
     1501 ccgataaagg atatcaggga aaaagcggat ctgatagatc gggctggcgc taccttttgc
     1561 ctgattatat tgcaggagga agatgtttcg gcggttggtg acgttctgaa ggtcgagata
     1621 gagcgtcgag cttagccgcc gtttcctgct gctggtgcga tagccgaatt tcgtgtcgag
     1681 acggaagtag tcgctcagcc gttgagagtt gtagtgcaac gtatccagga tctcgtagcc
     1741 ggccgcgaga gaagccccga cgttgacggg agttgcgtaa cggccgccga tggtggatag
     1801 ccggatatcc accgtgaagg cgttggcgcc gctgttaccc agtttccatt cttttcccgc
     1861 gagtgcattg aaaacatatt tataattgaa ggcagtattg cgttggatgc catcactacc
     1921 cttgtatttc gagtcgaaga cgctggccgt gaccaggaga taatagccat tgttcaggaa
     1981 gcgctcgagt gttagttcta cccccttatt tgtaccggtg cctttattta ccaatcccac
     2041 cttatcgggg aagccgaagt cggcgcccgc gttcagcatg gaaaagccgc tgggtgtcct
     2101 ctccacgggc acattaaaca gatattgata ataggcttcg gcctttaccc gccagccggg
     2161 gaggaagcgc cattcataac ccgcgacgaa gtgctgggcg cgggtgaggt ctaattttcg
     2221 attggaagag tcgcggatat tgtcggtttc gtcaaaggtt tgataataaa caggcagcgg
     2281 ttggagctgg ctgtgcaggc catagctcag cgaaatggat tgtcccgccg ggagttgata
     2341 cgtggccgag agccggggct cgagggcgga gctgttgttc gcatcgaaat gcatcccatg
     2401 caatcctccg acaatactga actgttcggt gacccggtat ttgaactgcc cgaaatactg
     2461 gagcagaagg ggatggccgt catagttgct gacggtgtcg aagggggcgc ttgcgggccg
     2521 cgcggttttg tcgaagacat ggatctttaa cccgaggtcc tcgcctgcaa tgccgatgcg
     2581 gtaagagaag cggctgtcga ctttttggtt gaggtaagtc gagaaccgta cggtggaagt
     2641 gcggttgtcg gaggtatact ccagccagcg atcctggtag ggcggtacgg ggtccgggta
     2701 gcggtactgg tcatagtgat cgagggtatg ggcataggtg acgacggtgc ggaggtaagt
     2761 cctgttgtcg atgtcgaggg tgtgtttgat accgaagtag gagaaattgc ttttgtcata
     2821 gctgtcttca tcggcctggc cgtagaagtc cgtggagtct gttttgctgc cgatgagatc
     2881 gatatgactg aggccgccca tgccgaagaa ggagaagcgg cccagcgcgc cgttgccggt
     2941 ggtgatatta aaggcccagt cctggtaatg ggggacggcc tttgtaccga tgttgatgcc
     3001 gatggcattg gtcaattgac ccagggcata gcgatacgag gcgagatagg cggcgccgtt
     3061 attcttgtta ttgagcggac cttcgactgt tgcttcgagt ccgctgaagg cgttcacctg
     3121 gagcgtcgtt tcgtggcggc tgctgttgcc gctgcggagg ttgatgtcga agacggctgc
     3181 ggtggcgttg ccgtattccg cggggaaggc gccggtgagg aagtcggagg tcttgagcat
     3241 attggtgttg agggcgctga cggggccgcc ggttgttccg agggtggcga agtgattggg
     3301 gctcggggag ggggagcctt cgatacgcca gaggacgccg gcggggctgt tgccccggac
     3361 gacgatgtcg ttccgggcgt cgctgttagc gacgacgccg gcgtagttac tgaccagttt
     3421 ggaagggtcg ttacggccgc ccgcataacg ggtgatatcg tcgatactga tcgagcgggc
     3481 gctgccggcg gagaactcat ttgtggcggc gcctttcttt gcagccgctg ccgtgatggt
     3541 aaacgtcttc agggtgacga gatgttgttc cagcgccatg tcgaggatga cctccttgcc
     3601 ggccgtgacc agtacttcgg ggatggacgc gccctggtag cccgcgctgc cataggaaac
     3661 ctgctggcgc ccgaggggga caccccgcag cacatagcgg ccgatcgagt cggtgacggc
     3721 gccaagattt gttcccacga cgaggacggt gacgccgggg agcggttttt ccgaggtctt
     3781 gtcgataacg acgccgcgga tggtctgggt gttttgggca gtggcgatca tcgacgccag
     3841 gaggaatagc agcaggctgg tgagcttttt catggttacc attttcaata caaacataca
     3901 gggcgggccc gggggcggca atcgaaaagg agtgggctgt ggaaaaggcg gtatgaaccg
     3961 gcgggaattg gcttccgaat cctggcaatt ggtcgtactt ttatcctatg cgttggcgca
     4021 acgataagac gggatttgac gaccggtggg tgatctccat cggcatcccg atcttttccc
     4081 tcgtgatacc gatcattttt tttggctggc gcatcggcat gccgccctat ctcgcctgga
     4141 atgtctattg gcctgccctc gtcataacgg cgacggtctg gatcggcagc cggtatatca
     4201 tgatctgggc gcgtacgcgg tatccccagt tcagggaggt gaagaaacga ctgtgggtac
     4261 agatgtcggt gatcctgctg ttcgtcctgt ttgtgaacaa tgtcctcggc gccttgctgg
     4321 actgcgatgc cgggttgcgg gacagcgcgc accagctgac ccaggcggat atcctgatcg
     4381 attccaatgc ggcgaccatc ctctgcaccc tgctcgtgac cgccatctat gagagtatct
     4441 acttcgtgag cgaactacga aaatcgatag aagagaagga gttgctgaag cgggagagcc
     4501 tgcaggcgca ggtgaatgcc ctgaagatcc aggtgaaccc tcattttttg ttcaacac
//
LOCUS       DATGFG010000557         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1180984, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000557 DATGFG010000000
VERSION     DATGFG010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180984"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VKQ52_10465"
     CDS             <1..444
                     /locus_tag="VKQ52_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87657.1"
                     /translation="FSAVNDFAAPAPTATNPYPVSPWTAFLATPSGGADQVLTLSFSP
                     LKFPPWTRGKTISATSMTLFTVGWQPGNFVVAPLAPLPAAQLVMNPVAGVTEPNVCSA
                     NIGLPPGTSLGTWSFELQLQGAADFRSLSKSQIGDLIIVINFSAT"
     gene            454..804
                     /locus_tag="VKQ52_10470"
     CDS             454..804
                     /locus_tag="VKQ52_10470"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLZ87658.1"
                     /translation="MEITLPLLVRQALERLIRAFDPEQVLLFGSVAKGTNDDNSDVDL
                     LIVTRTVDDVSLLRRRARQLTAGCFPAVDIVFVSSDELANAEILKSPFLASITEKGLV
                     VYLKVQSEAVRPNN"
     gene            1240..1593
                     /locus_tag="VKQ52_10475"
     CDS             1240..1593
                     /locus_tag="VKQ52_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87659.1"
                     /translation="MEVRLLPKEPASRFDVSIILQRLLLQIRDKGIAIHPMTQVLEEN
                     RFSQNVNGELGITEPIQFIAHRPKYFPSLGLHLIHPIVWIIYTLVLYTSIRIRISQTP
                     LLRSVEKNFRGDKFE"
     gene            2069..3991
                     /locus_tag="VKQ52_10480"
     CDS             2069..3991
                     /locus_tag="VKQ52_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HLZ87660.1"
                     /translation="MNLPKITSPSIPVAKRNQLFGIALLLTSLLSVIRVNAQTGLWTW
                     ISGDNVANITGIYGTKGTGAATNKPGNRDSHSGWKDAAGNFWVFGGWDFNGNAYNDLW
                     KYTVSTGHWTWVSGDNTQNNAGIYGTKGTAAAANKPGARFGQSAWVDAAGDFWIFGGQ
                     GYDVNGRYGYLNDLWKYHPATGQWTWVSGDNTRRNTGVYGTKGTAAAANKPGARYYQA
                     GWIDATGNSWLFGGYGYDGAGNQGGLNDLWKYNPTTNQWTWVSGDNTRNKTGVYGTKG
                     TAAAANKPGNRDSHSGWVDASGNFWIFGGYDDNSGSLDNDLWTYNTATGQWTWVSGDN
                     TQNNPGVYGTKGTAAAANKPGARYGQSATVDGGGDFWVFGGQGYDASGNPGVLNDLWK
                     YNPTTNQWTWTSGDNTQGSTGVYGILGTASAANKPGGREYLSAWIDASNDLWVLGGYD
                     NNISDFNDLWKFNSLLPLPIREVSLEGINRSNENLISWQTIGEENTARFEVERSTDGT
                     DFSALGSVSAIGFGNNSYSFTDAQLPESKVYYRLRIEDSNGAFSYSRVIVLPGGTQNS
                     KFTVYPNPAKNSITLNIADFSLLNTTVKLFDAGGRLVGAFRISGGQQYIDLSRIPRGI
                     FLLQLTNGETVKIVKE"
     gene            3996..4241
                     /locus_tag="VKQ52_10485"
     CDS             3996..4241
                     /locus_tag="VKQ52_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87661.1"
                     /translation="MFAFSTNQVQTNNISKNTAATTIGIFVRKNAGALSLTIEDDGKG
                     FDREHANGGIGLANIRNGVEGFNGTLTITTQLKELWR"
BASE COUNT         1158 a         1068 c         1101 g         1024 t
ORIGIN      
        1 ttcagtgcag taaatgattt cgctgctccc gcgcctaccg caaccaaccc ttaccccgta
       61 agtccatgga ctgcttttct ggcaacgcct tccggcggag ccgaccaggt gcttacccta
      121 tctttttcac cgttgaagtt cccgccctgg acgcggggca agacgatttc tgccacatcg
      181 atgacgctgt ttactgtcgg ctggcagccg ggtaacttcg tcgtcgcccc gcttgcacca
      241 ttgcctgctg ctcagctggt gatgaacccg gtagccggcg taacagagcc aaatgtttgt
      301 tccgcgaata ttggactacc acctggtacc tcgttgggca cctggagttt tgagttgcag
      361 ctgcaaggcg cggcagactt cagatcgctt tccaaaagcc agatcggcga tttgatcatt
      421 gtcatcaatt tttcagcgac ataaacccat tatatggaga ttacccttcc actcctggtg
      481 aggcaggcgc ttgagcggct aatccgcgca ttcgatcccg aacaggtgct gttgttcggt
      541 tccgttgcca agggcaccaa tgacgataat agtgacgttg acctgctgat cgtgacaaga
      601 acggtggacg acgtatcact actgcgacgg cgggcccggc aattgacggc gggctgtttt
      661 ccggcggtgg atatcgtttt tgtcagttcg gatgaattgg caaatgctga aatccttaaa
      721 tctccatttt tagcttccat taccgaaaag ggcttggttg tatacctaaa ggtccaaagc
      781 gaagcagtac ggccaaataa ctgaggaggc actgcggaag cgtcgtttgc agcaaaccat
      841 tccgaacgga tcccgggata aaaaggtggt gccggtgttc aggctgccat gcattacctt
      901 catgtgacca ctatcacccg gttggttgac cattataaat cagtaaaatt ccctccagta
      961 ttagtttcag gtaaaagtat atatgaaatt taaactgtta tcgttattga tgctgttttc
     1021 tgcaccagca atcgctcagc aggaaggcga aggcactttt attaaaccgg cgccggcgaa
     1081 ctatgaagtc aggcaagctt tcgagcggtg gaccccgcac agcgagagac cattttgtcg
     1141 ataggcgcat ttattcagaa cctggattat gccgcggatc atttcgacta ccattgccag
     1201 ggaaacctcc tggcgaggtc taaccagggt gagaacataa tggaagtacg acttttgccg
     1261 aaagagcctg cttcccgctt tgacgtttca ataattttgc agcgtctttt gttacagata
     1321 cgcgataaag gtattgccat tcatcctatg acgcaggtgc tggaggaaaa tcgcttttcg
     1381 caaaatgtaa atggtgagct ggggataaca gaaccgatac aattcattgc ccatcggcct
     1441 aaatattttc cctctctagg tcttcacctt attcatccga tcgtctggat catttatact
     1501 ttggtactgt atacatcaat caggatcagg atcagtcaaa cgccattact ccggtcagtc
     1561 gaaaagaatt tccgcggaga taaatttgaa tgaaattgct ctatatggcc aacatcagta
     1621 tcaccggctt ttagtgtggg aaagccaact aatgattaaa aagcagtaga attactacag
     1681 ctccaacacg catttatatc atattttaag tgttttcacc tatatgcaat attgtttgca
     1741 ggcttgcctg ccgagtcggc aggtttcttt ctgataacca gaatggccgg atcctgccgg
     1801 ctgaatgtga ctcctgttgc ttacgcatga gttcgacagc cagcctggaa agtagacctg
     1861 gttgatccaa aaaatggact tcactccctt aaaaagaatt ttcccatcat tggaccggct
     1921 tcaaattgct gatgatcaaa tcattagtaa ccaaattatt acccgcagaa ataagtctat
     1981 catgtaatat ttgctacata catatactta gtagtgatat gtttcgttta tgaggggcta
     2041 taaatccaga catatcctga atcatccaat gaatctacca aagatcactt ccccgtcgat
     2101 ccctgtagca aagagaaatc agcttttcgg gatcgcttta ttacttacca gcctacttag
     2161 tgttatccgg gtaaacgcac aaacaggtct gtggacctgg atcagcgggg ataatgtcgc
     2221 caatatcacc ggcatttatg gaaccaaagg aacaggcgcg gcaaccaata aacccggcaa
     2281 cagggacagc catagcggtt ggaaagacgc cgccggaaat ttctgggtct ttggcggatg
     2341 ggattttaat ggcaatgctt acaatgatct atggaagtac accgtgtcca ccggccattg
     2401 gacctgggtc agcggagaca acacccaaaa taatgctggc atatacggca cgaaaggaac
     2461 tgctgccgca gccaacaagc ccggtgccag attcggacaa agcgcgtggg tagacgctgc
     2521 cggcgatttc tggatcttcg ggggacaagg atatgatgtg aacggaaggt acggatacct
     2581 caacgacttg tggaaatacc atccggcgac tggtcaatgg acctgggtca gtggggacaa
     2641 tacccgaagg aacaccggcg tatacggcac gaaagggacg gcagccgcgg cgaataaacc
     2701 gggcgcaagg tattaccagg caggctggat cgacgccacc ggcaatagct ggctctttgg
     2761 cgggtatggt tatgatggag cgggcaacca gggtggcctc aacgacctgt ggaaatacaa
     2821 tccaacgacc aatcaatgga cctgggtcag cggggataat acgcgcaaca aaacaggggt
     2881 ttatggcacc aaggggactg ccgccgcagc caataagccc ggcaacaggg acagccatag
     2941 cggatgggtg gatgcttccg gtaatttctg gatcttcggc ggctatgatg ataatagtgg
     3001 aagcttagac aacgacctgt ggacgtataa cacggcaacc gggcagtgga cctgggtcag
     3061 cggagacaat acccaaaata atccgggagt ctacggcacc aaaggtactg cggccgcggc
     3121 gaacaagccc ggagcaaggt atggccaaag cgctacagta gatggcgggg gagatttctg
     3181 ggtatttggc ggccagggtt atgatgcatc cggtaatccc ggggtattga acgatctctg
     3241 gaaatacaat cctacaacca atcaatggac ctggaccagc ggagataata cgcaaggcag
     3301 caccggcgta tatggtatcc tggggacagc ctccgctgcc aacaagcccg gtggaaggga
     3361 atacctcagc gcctggatag acgcttccaa cgatctctgg gttttgggcg gatatgataa
     3421 caacatcagc gatttcaacg acttgtggaa attcaattcc ctgctgccgc tgcctatccg
     3481 ggaggtttcg ctggaaggaa taaaccgttc caatgaaaat ttaatatcct ggcagaccat
     3541 cggcgaagaa aataccgccc gctttgaagt ggaaagaagc actgacggaa cagatttctc
     3601 tgctctggga agcgtttcgg cgattggctt cggcaacaat agttactcct tcaccgacgc
     3661 tcagttaccc gaaagtaagg tatactaccg cttgagaatc gaggatagca atggagcctt
     3721 ctcttattcc agggtcatcg tcttgcccgg tggtacccaa aacagtaaat ttaccgtcta
     3781 tcctaatcct gcaaagaaca gtatcaccct gaacatcgcg gatttcagcc tgctgaatac
     3841 gactgtaaaa ttgttcgatg cgggcggcag gcttgttggt gcatttcgga tttccggtgg
     3901 acagcaatac attgatctaa gccgcatacc caggggaatt ttcctgctgc aattgaccaa
     3961 tggtgaaacc gttaaaattg taaaggagta gaattttgtt tgccttttca accaaccagg
     4021 tacaaaccaa caatatctct aaaaatactg cggccaccac aataggcatc tttgtcagga
     4081 aaaacgccgg tgcacttagc ctgacaatag aagacgacgg taaaggcttc gaccgggaac
     4141 atgcaaacgg gggcattgga cttgccaata tccgcaacgg cgtggaaggc ttcaacggga
     4201 cattgaccat caccacacag ttgaaggagc tgtggcgatg aaatgaaacg gattcactgc
     4261 tcaccgcatc gccggtatca ccaaaacagg ccgcccggcg tgccggatga tatgctccgc
     4321 cacgctgccc attagcaaat gcgtcacacc t
//
LOCUS       DATGFG010000558         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1375649, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000558 DATGFG010000000
VERSION     DATGFG010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375649"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VKQ52_10490"
     CDS             complement(<1..608)
                     /locus_tag="VKQ52_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HLZ87662.1"
                     /translation="MQSGQLQPADLDPGLFQFIKDKLITGAEGGWGMSFSKVDYDTPD
                     YKLLTTMQQNVYAFAGAKTYQELRALNDLLYDSGGRIVSFGQFRDKIEQYRQQALQIN
                     DQYNKQWLQTEYNMAIAQGQMARKWQGYQENKDLYPNIKYMTAGDARVRPAHALLDGT
                     VKPVDDPFWDKYYPPIDWGCRCDTVSSEGPATKGMDSPPVNVT"
     gene            complement(725..2098)
                     /locus_tag="VKQ52_10495"
     CDS             complement(725..2098)
                     /locus_tag="VKQ52_10495"
                     /inference="COORDINATES: protein motif:HMM:NF017850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HLZ87663.1"
                     /translation="MEITNSSKAGGITVKTNDPKDIVIQNLVIRPVIRSKADVQDWRS
                     AQIQAESTLGVRRWLYDLYADALLDPVLTSEINKRCSAVTKKLQFKDAAGKENDQVKQ
                     LLKKKRFKKMLREIILSKMWGVTVLEFKPDTPDLFYSVPRKHIRPRDKRIVWEQYETD
                     GETGIVYTDPPYNNYIIEVCEDSEDLGLILKAVPYVLIKRGDIGDWAQFVEVFGMPFR
                     KAEYDPFDETSRALMEKALDTAGSAPWMLLPKGGTFELIQNNGTAANGDIYEKLKDAC
                     DEQILILITGSTETTKSSKSSGYAQSKTHENTKQDVLDEDRDFVVSVLNEDFIPLMDN
                     HGWGLAGGEFYYEDEDPLEDRQQKVAVLQSMKQMGTPVSDDMVYEASGFAKPDNYNEL
                     KQAPPPAPPGDPGEGQEDTEETTSDEGGTPGTGKANKKPPKDQLSGKGFWYHLRSTLA
                     DFFDPAP"
     gene            complement(2189..2614)
                     /locus_tag="VKQ52_10500"
     CDS             complement(2189..2614)
                     /locus_tag="VKQ52_10500"
                     /inference="COORDINATES: protein motif:HMM:NF018704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp36 family protein"
                     /protein_id="HLZ87664.1"
                     /translation="MAFLTKDDLVSHIKAEVIDFITESDDTVVGEAIAAAIEEAKGYL
                     SDYDIDTIFTATGTDRNPIVLLYTKDIAVWHFINLANPNIEISFRETRYNLAIEWLEK
                     VQSRKTNPAIPLRPSLPDGSPFQWDMVRAGSNTARRGRY"
BASE COUNT          556 a          754 c          681 g          761 t
ORIGIN      
        1 gtaacattga ccggcggact atccattcct tttgtagcag gcccctcgct ggacacggta
       61 tcgcaacggc aaccccaatc aatcggcgga taatatttat cccaaaacgg atcgtccacc
      121 ggcttaacgg ttccatcgag caaggcgtgg gcaggacgga cgcgggcatc cccggccgtc
      181 atatatttga tattcgggta caggtctttg ttttcctggt agccctgcca cttcctggcc
      241 atctgccctt gcgcgatggc catgttgtac tcggtttgaa gccactgctt attatattgg
      301 tcattgatct ggagggcctg ctgccggtac tgctcgatct tatcccggaa ctgcccgaaa
      361 cttacgatcc tgccaccgga atcatagagc agatcgttca gcgcccggag ttcctggtag
      421 gtcttggctc cggcaaaggc gtaaacgttt tgctgcatgg tggtaagcaa cttgtaatcc
      481 ggggtatcgt aatccacttt gctaaagctc attccccatc caccctctgc cccggtgatc
      541 agtttatcct ttatgaattg gaacagaccg ggatcgaggt cggccggttg tagctggccg
      601 ctctgcaacc ggttaagtat ttcctcaaag gtgctttccg gttctccggc agatagtttt
      661 tcatggatat gagaacagcg ataaagatca gtcaacaacc tggccagccc cgtatgccct
      721 atagctacgg ggccgggtcg aaaaaatccg ccagggttga gcgcaagtga taccaaaagc
      781 ccttccctga aagttgatcc tttggcggct tcttattcgc cttgcctgtt cctggagtac
      841 caccttcgtc cgaagttgtt tcttccgtat cctcctggcc ctcgcccgga tcaccagggg
      901 gagccggtgg cggggcctgt ttcaattcgt tgtaattatc cggctttgcg aatccgctgg
      961 cttcatacac catatcgtcg ctcacgggcg ttcccatttg cttcatggat tgcaggacgg
     1021 ccactttttg ttgccggtcc tccagcgggt cttcgtcctc atagtaaaat tcccctccgg
     1081 ccaagcccca tccgtggttg tccatgaggg gtataaaatc ttcgttcaat acgctgacga
     1141 caaaatccct atcctcatcc agtacgtcct gcttggtatt ttcatgggtc ttgctttgcg
     1201 catagccgct gctcttggag gatttggtcg tttccgtact cccggtgatc aggatcagga
     1261 tttgctcgtc gcaagcgtct ttcaactttt cgtatatatc cccattggcg gcagtcccgt
     1321 tgttctggat caattcaaac gtcccccctt tgggcagcag catccaggga gcggacccgg
     1381 cagtatccag ggccttttcc atcagcgccc tggaggtttc atcgaaagga tcgtattccg
     1441 cttttcggaa cggcatcccg aacacctcga cgaattgcgc ccaatcccca atgtctcccc
     1501 gctttatcaa aacgtaggga acagctttta agatcagacc caggtcctcc gaatcttcgc
     1561 atacctctat tatatagtta ttatagggtg ggtcggtata aacaatgcct gtttccccgt
     1621 ccgtctcata ttgctcccac acgatccgtt tgtccctggg acggatatgt ttacggggaa
     1681 cggaatagaa caggtcgggg gtatccggtt taaactccag gacggtcacc ccccacattt
     1741 tggaaaggat gatctcccgg agcatcttct taaatcgctt tttctttaaa agctgtttca
     1801 cctggtcgtt ttccttcccg gccgcgtcct tgaattgtaa tttcttggtg acggcgctgc
     1861 atcgtttatt gatctcggag gtgagcaccg ggtccagcag ggcatcagca tagaggtcgt
     1921 acagccagcg gcggaccccc agggtacttt ctgcctggat ttgggcgctc cgccagtcct
     1981 ggacatcggc tttgctgcga atcaccgggc ggatcaccag attttggatc acgatgtcct
     2041 tcggatcatt cgtttttacc gttatacccc cagctttcga actattcgtt atttccattt
     2101 taaagcctct ttttatcccg gacgatgaaa tacccgtccg ggaagtgatc gtcgatttta
     2161 aacacgatta aatgggtttt aaacagggtc aatacctacc cctcctggcg gtattacttc
     2221 ccgctctgac catatcccat tggaaaggtg atccgtctgg tagggatggc cgtaagggga
     2281 tggccgggtt cgtctttcgg gactgcactt tttccagcca ttcgatggcc aggttgtacc
     2341 gggtttcccg aaaggaaatt tcaatgttcg ggttggccag gttgataaag tgccataccg
     2401 caatgtcctt tgtatagagt agtacaatcg ggttgcggtc cgtacccgtg gccgtgaaga
     2461 tggtatcaat gtcatagtcg ctcaggtatc ccttcgcttc ctcgatggcg gcggcaatgg
     2521 cctcgcccac tacggtatca tcggattcgg taataaagtc gatgacctcc gctttaatgt
     2581 gcgataccag gtcgtccttt gtcagaaaag ccatactaaa attttttaat gtttttggac
     2641 cgtttgccaa accgaagcgg ggctgtttgc tccagtgtct tattgttaat aatccatacc
     2701 gcgccttcca cagcatccgg gaagtcatcg tgcgccctgc tggtaagatc gg
//
LOCUS       DATGFG010000559         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_985897, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000559 DATGFG010000000
VERSION     DATGFG010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985897"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="VKQ52_10505"
     CDS             complement(<1..1487)
                     /locus_tag="VKQ52_10505"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HLZ87665.1"
                     /translation="MNVQEFAIRLKDQTTGQMGRVVSMTESGMAKFVRSVKMGEDELE
                     KFAHKAESLKDRVGETFRDLLRGLAPFLAAFGVGALVEGLGEVAIHAEQTKVQFEVLL
                     GSASKATTMIENLHAFADKTPFQFNDLAEASKTLLSFGVSAQNTMPVLKRIGDVSGGN
                     AERLQMLSSAFGKVAAFQRLTGREMMEFIHAGWNPLRDISEATGLSMEKLQEHMRKGE
                     IGINMVGAALEHATGPGGRFHDMLLKQSETLGGKWSTLKDHIHELALQAATALAPLLK
                     QLVEFGEWLLQNKEYVIGLAAGIGAYVLIVQGATIATNIWRFAQLLLNTAMKMNLIGL
                     IIGAIIFLVFWIKHAWEHFGWFRGAVYGAWEALKGLGTLIKDFLVDTLVNYLKGIWGL
                     GEALVKFFKGDFSGAWESAKQATKDLIGVSTIQHAVENAKKLGQNVAAGYNQGVREMA
                     TAHGKEGTTNPEKENGIAGAAFKFSKPGGEDTADSISKNGVRNIH"
     gene            complement(1665..1985)
                     /locus_tag="VKQ52_10510"
     CDS             complement(1665..1985)
                     /locus_tag="VKQ52_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87666.1"
                     /translation="MNNETKALRSGELPAEQESQLKEKYPTLFKLEFEDKVAYLRKPD
                     RATLKAAMSKVLTDPIGFAEVILKNCWLDGDEDIKKNEEYIMGSVSPLNNIVNARTAE
                     LVKL"
     gene            complement(2009..2437)
                     /locus_tag="VKQ52_10515"
     CDS             complement(2009..2437)
                     /locus_tag="VKQ52_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87667.1"
                     /translation="MAEYFNSSEYAWSDLQVVLNGKVITGIRGIKHSMEQDLEEVYGA
                     GVQPLAIQEGNMKYEGEITLLKMEADDLETAVLAAGYPSVLKAKGLSIVVHFENDEGY
                     VNTKIVSKLKISKWEESMKQNDKFMEIAVPFKALSIQKVS"
     gene            complement(2460..>2831)
                     /locus_tag="VKQ52_10520"
     CDS             complement(2460..>2831)
                     /locus_tag="VKQ52_10520"
                     /inference="COORDINATES: protein motif:HMM:NF022218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HLZ87668.1"
                     /translation="DNTATASTDDLSSFARGRIIDKAIRTTYATYVNEINEHFDVDSN
                     GRIPQPVIKYLENEIIQAIEGAEGASLSNADNAVQAYIDPNQNVLSTNTTKVKVRLIP
                     KYYNKEIDVDLGFTNPQVITN"
BASE COUNT          597 a          802 c          660 g          772 t
ORIGIN      
        1 atatggatat tgcgcactcc gtttttgctg atactatctg cagtatcctc cccacccggt
       61 ttagaaaact taaaagcggc cccggcgatg ccgttctcct tttccgggtt ggtcgttcct
      121 tctttgccat gcgcggttgc catttctctt actccctggt tatatcccgc cgcaacgttt
      181 tgcccgagct tcttggcatt ttccacggca tgctgtatgg tgctcacgcc aatcaggtct
      241 ttggtagctt gcttggcgct ttcccaggct cctgaaaaat cccctttaaa aaactttaca
      301 agggcttctc ccaggcccca tatacctttc aggtaattga cgagggtatc gaccagaaag
      361 tctttgatca gtgtccccaa accttttaag gcttcccagg ctccatacac ggccccccga
      421 aaccatccaa aatgttccca ggcatgttta atccaaaaca ccaggaagat gatcgcgcca
      481 atgatcagac cgataagatt cattttcatg gcggtattta acagcagttg agcaaagcgc
      541 caaatattgg tggcgatggt cgcaccctgc acgatcagga cgtaagcgcc gataccagcg
      601 gccaggccga tcacatactc tttgttttgg agtaaccact caccgaactc cactagctgt
      661 ttcagcaagg gagccagggc ggtcgcggcc tgcaaagcca gttcatggat atgatctttg
      721 agcgttgacc acttgccacc taatgtttcc gattgtttga gcaacatatc gtgaaaccga
      781 ccgcccggcc cggtcgcgtg ctctaaagca gccccgacca tgttgatccc gatctctcct
      841 ttgcgcatgt gttcctggag cttttccatg ctgaggcccg tggcctcgga aatatcccgg
      901 agcggattcc acccggcatg aatgaactcc atcatttcgc gccctgtcaa ccgttgaaag
      961 gcggccacct tgccgaaggc cgacgacaac atttgcaagc gttcggcatt gccgccgctg
     1021 acatcgccaa tccgtttgag gaccggcatc gtattttgag cactgacgcc aaaagaaagg
     1081 agcgttttgg acgcctccgc caggtcgttg aattgaaagg gcgttttgtc cgcaaaggcg
     1141 tgtaggttct cgatcatggt cgttgccttc gaagcgctcc cgagcaatac ctcaaattgt
     1201 accttagtct gctcggcatg gatggccacc tcgcccagcc cttcgaccaa tgccccgaca
     1261 ccaaaggcag ccaagaaagg agccaatccc cgaagcaggt cgcggaaggt ttctcccacg
     1321 cgatctttga gcgattcggc cttatgggcg aacttctcca attcatcctc tcccattttc
     1381 acgctacgca cgaactttgc cataccgctt tccgtcatac tgaccacccg gcccatttgc
     1441 ccggtcgtct ggtctttcag tcgtatggcg aactcctgta cgttcatggt ggttttacaa
     1501 tttgggttgg ctccctgcct cggccttgcg aatgttctcc agtatcttaa tagtctgtgc
     1561 ccactgctcg tcgtctaacc gggaggggtc gatcttcagg aaatactgca actgtatatc
     1621 gaaatagagg atggggttat cctcatactt ccccgaagcc tcccttagag ctttaccagc
     1681 tcggccgtgc gggcattaac gatattgttc aaaggggata ccgaccccat gatgtattcc
     1741 tcattcttct ttatgtcctc gtcgccgtcc agccagcaat ttttcaggat gacctcagca
     1801 aagccaatag gatcagtgag cactttgctc atggccgctt tcagcgtagc ccgatccggt
     1861 ttgcgcaggt aggcaacctt atcttcaaac tccagcttaa acagcgtggg atacttttct
     1921 ttgagttgac tttcctgttc ggccggaagt tcgccggacc gcagggcttt cgtttcgtta
     1981 ttcatgggta cttggttgta atggattatt aggatacttt ttgaatggaa agggctttga
     2041 agggaacggc aatctccatg aacttgtcat tctgtttcat cgattcttcc cacttgctga
     2101 tcttcagctt ggatacgatc ttcgtgttga cgtagccctc atcattctcg aaatggacga
     2161 caatagatag cccctttgct ttcagcacgg aaggataccc ggctgccagg accgccgtct
     2221 ccaggtcgtc ggcttccatc ttcaataagg taatctcccc ttcatacttc atattacctt
     2281 cctggatcgc cagcggttgg acacccgccc catacacttc ctccaggtct tgttccatcg
     2341 agtgtttaat gccccggatg ccggtaatca ctttcccgtt taacactacc tgtaaatcgc
     2401 tccaggcata ttccgaactg ttaaaatatt ctgccattta aaagccgttt aaacgctgtc
     2461 taattagtga tgacctgcgg gttggtaaag cccaggtcta cgtcgatctc tttattgtaa
     2521 tacttgggaa tgagccgcac ctttactttt gtggtattcg tgctcagtac attttgattc
     2581 ggatcgatgt acgcctgtac tgcattgtcc gcattgctca ggctggcccc ctcggcccct
     2641 tcgatggcct ggatgatctc attctccagg tacttaatca ccggctgcgg aatacgccca
     2701 ttactatcca cgtcaaaatg ctcgttgatc tcattcacgt aggtagcata ggtggtgcgg
     2761 atggccttgt caatgatccg gccacgggca aaggagctga ggtcgtcggt tgacgcggtg
     2821 gccgtattgt c
//
LOCUS       DATGFG010000560         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_32503, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000560 DATGFG010000000
VERSION     DATGFG010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32503"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="VKQ52_10525"
     CDS             <1..1566
                     /locus_tag="VKQ52_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HLZ87669.1"
                     /translation="LLTIYGVGNLHAAISYRKYKERFQRDTGFKQEERQIETEYSLHF
                     RATYQLNGRTRKSWVNIINPRRGVLIMGSPGSGKSWFLIEPMIEQLIRKGMALLVYDF
                     KFPSLTQFTHDQFLQHQNNYPPGTRFYCVNFTDLSHSHRCNLLDPVTLENKNDAMGIS
                     RTILLSINRIWAQKQGDFFVESSINYLAALIWFLRIYKDGAFCTLPHVIELSKASYEE
                     LFTILNAEPETRGLIGPFKEAFLNRTMEMLDGQVASARIPLNRLDSPDFYYVLSGNDL
                     KLDINNPSFPAVLCLGGDPERQEALAPVLSLFIDRLNRRINKAHRYPCALVLDEFATV
                     RATGVLNTIATGRSNNISSILAVQDLTQLTQQYSHDEAKQIMNTADNLICGQVSGETA
                     RLVSDRFHTHAYLKTTVSTNSSDISTSKTEHTEDAITPATLANLSSGEFIGVVADEPE
                     ARLELKGFHASFVKRPDSKTKQHPLPAVGRVDAKLLKENFLRIGNETTTIIKDEMKRI
                     LNDPALKQYIVKR"
     gene            complement(1913..>3014)
                     /locus_tag="VKQ52_10530"
     CDS             complement(1913..>3014)
                     /locus_tag="VKQ52_10530"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HLZ87670.1"
                     /translation="GREEVLRRLSDPFWFQSFGAVMGMDWHSSGITTSVMGALKRAIN
                     PHSRELGIYICGGKGSASRQAPAEILKVCESTGLDGVELVRSSKLSAKVDNTVVQDGF
                     QLYMHCFVLSREGQWTVIQQGMSPGNSMARRYHWHSAGLRSFVEEPHTAICGENQGEI
                     LNLADRGADPARKGVLALTREKPEMMMQDVQQLVLPAHHDVRTEDVDLRRLGAVLWLA
                     HDRQPADFEELVLLEGVGPRTLQSLALVSEVIHGAPSRFRDPARFAFAHGGKDGHPFP
                     VPIDVYDETIGILQTAVQKAKVGQSDKLEAVKKLNLLARRVEDQFTPTGSGLEEWIEK
                     ERADSWKYGGRTVFEKAKPPEGVVRARQLQLF"
BASE COUNT          776 a         1038 c          635 g          565 t
ORIGIN      
        1 ctgctgacca tttatggggt cggcaacctg cacgccgcca taagctatcg taagtacaag
       61 gaacgcttcc aacgcgacac cggcttcaaa caggaggaac ggcagatcga aacggaatac
      121 tcccttcatt ttcgcgcaac ctaccagcta aatggccgca cccggaaaag ctgggtcaac
      181 atcatcaacc cgagacgcgg cgttctcatc atgggatccc ccggctccgg caaatcctgg
      241 ttccttatcg aaccgatgat cgaacaactc atccgaaaag ggatggccct gctggtatat
      301 gatttcaaat ttccttccct cacacaattc acccacgatc agttcctgca gcaccaaaac
      361 aactacccgc cgggaacccg gttctactgc gtcaacttta ccgatctgtc ccacagccat
      421 cgctgcaacc tcctcgaccc ggtgaccctc gagaacaaga acgacgcaat gggtatcagc
      481 aggaccatcc tgctaagcat aaaccgcatc tgggcgcaaa aacaaggaga cttctttgtg
      541 gaatcgtcta tcaactatct ggccgccctg atctggtttc tcaggatata caaggatggc
      601 gccttttgca ccctgccgca tgtcatcgag ctttccaaag catcttacga agagcttttt
      661 accatcctca acgcagaacc ggaaacccgc ggcctgatag ggcctttcaa agaagccttt
      721 ctgaacagga ccatggaaat gctggacggc caggtggcct ccgcccggat acccctcaac
      781 cgcctggact ccccggattt ttactatgtc ctcagcggta acgatctcaa actggatata
      841 aataacccgt ccttccccgc cgtcctttgt cttggcggag acccggaaag acaggaagca
      901 ctggcgccgg tgctttcctt gttcatcgac cggctcaacc ggcgtatcaa taaagcgcac
      961 cggtacccct gcgccctggt actcgacgaa tttgcaaccg tccgggcaac cggcgtcctt
     1021 aacacgatcg caaccggccg gtccaataat atatcctcca tcctcgccgt gcaagacctt
     1081 acccaactga cccaacaata ttcacacgat gaagcaaaac agatcatgaa caccgccgac
     1141 aacctcatct gcggtcaggt cagcggggaa acagcccgcc tggtcagcga tcgattccat
     1201 acccacgcct acctgaagac caccgtctcc accaatagct ccgatatatc gaccagcaaa
     1261 accgaacaca ccgaagacgc catcacaccc gcaacactcg ctaacctctc ttccggcgaa
     1321 ttcatcggcg tcgtagccga tgagccggag gccaggctgg aattaaaagg atttcatgcc
     1381 tcctttgtaa aaaggccgga tagtaaaacc aaacagcacc cccttccggc cgtcggcagg
     1441 gtagatgcca agctcttaaa ggaaaacttc ctccgcatcg gcaacgagac caccacgatc
     1501 ataaaagacg aaatgaaacg catcctcaac gacccggcac taaaacaata tatcgtcaag
     1561 cgatagacag ctatcccgat agccatacac acccccaaaa acacatgaaa ataattttct
     1621 acacactctc taaaatcctc taaacatacc ccactaaaga aaacccttac cccatcgaat
     1681 cactcacttc cttgtgtatg gatcacgcga gcgcactgta catttatcgc gtgcaaaatc
     1741 tctaaccaag gaccaggtat tcggtatgaa cgcttccaca aaagaaaaca ctgaaaaagc
     1801 aagagcactg aaaaagctca tgcggaaatg gcttacgaca caggggacag aaagaaaggc
     1861 tagcttactg atagaaaaaa atccggagct gaaacgtaga aagaccgcca gctcaaaata
     1921 actgcaactg cctcgccctg acaacgccct ccggcggctt tgccttctca aagaccgtac
     1981 ggccgccgta tttccaggag tctgcgcgct ctttttcgat ccattcctcc aggccgctgc
     2041 cggtaggcgt gaactgatcc tccacccgcc gcgccagcaa attcaatttc ttcacggcct
     2101 ccagcttgtc actctgcccc accttcgcct tctgcaccgc cgtttgcagg atgccgatcg
     2161 tctcgtcata gacatcgata gggacaggaa aaggatgccc atccttaccc ccatgcgcaa
     2221 aagcaaaacg cgccggatca cgaaaccgcg acggcgcccc atggatgacc tcgctgacaa
     2281 gcgccagcga ctgcagcgtc cgcggcccca ccccttccag caacaccagc tcctcgaagt
     2341 cggcaggctg ccggtcatgc gccagccaga gcacggcgcc caacctgcgc aggtccacat
     2401 cctccgtccg gacatcatga tgcgccggca acaccagctg ctgcacatcc tgcatcatca
     2461 tctccggctt ctcccgcgtc aacgccagca cccccttcct cgccggatcg gccccccggt
     2521 cggccaggtt caggatctcc ccctggttct cgccacagat ggccgtatgc ggctcctcca
     2581 caaaagacct tagcccggcc gaatgccagt gataccgccg cgccatggaa ttacccggac
     2641 tcatcccctg ctggatcacc gtccactgcc cctcccggct aaggacaaaa caatgcatat
     2701 acaactgaaa accgtcctgc accaccgtat tatccacctt cgcactcagc ttgctgctcc
     2761 gcaccagctc caccccatcc agtcccgtac tctcgcatac cttaaggatc tccgccggcg
     2821 cctgccggct ggcactcccc ttgcccccgc agatataaat gcccaactcc cgcgaatgcg
     2881 ggttgatcgc ccgcttcaac gcccccatca ccgaggtggt gatccccgac gaatgccagt
     2941 ccatccccat caccgcccca aaactctgaa accagaaagg atccgatagt ctgcgcaaca
     3001 cctcctcccg ccca
//
LOCUS       DATGFG010000561         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1613300, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000561 DATGFG010000000
VERSION     DATGFG010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613300"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VKQ52_10535"
     CDS             <1..335
                     /locus_tag="VKQ52_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HLZ87671.1"
                     /translation="TIAVSFTENGIRRTVYRDVNSGGSFGASPLRQQIGIGRAEKIDR
                     LEITWPTTGLIQSFSNIAPRQFIHIREGENEIKTMNIRAFPFKCLDLQKNSAACEPPM
                     VGITHSVK"
     gene            344..2926
                     /locus_tag="VKQ52_10540"
     CDS             344..2926
                     /locus_tag="VKQ52_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HLZ87672.1"
                     /translation="MNKLALSAILLFGFTLFGKTQTIAKHALPSLAKHALPSPTQSLT
                     INDSGYFEKRGVNLLVFTNQYNGMFYDEKTAGIELIHHGVRTCTGGAVRLQNTPEQWD
                     LIPILVSRKVDKANNSIEVVLKYREYDFLSRIRVSAKDSGVEISVYLDQPLPEKLEGN
                     AGFNLEFLPAAYFEKTYLADGRPGTFPLYPAGQTKMEPLAQKIPQFAGHTTFDDRGRN
                     EFIVPLPLATARSMVLAPEDPAREVTIRSQDADLMLFDGRNLAQNGWFILRSLLPAKK
                     TGKVLTWYLEPSAIPDWKRKPVIGFSQVGYRPNQEKTAVIELDKNDAPLPTASLFQVT
                     AEGTPVEKWKGSVHSWGRYLRYTYAKFDFSAVKDPGLYYIQYGQERTNTFRISPDVLD
                     DSWHPTLDVWLPVQMDHMEVNEAYRVWHGVPFMDDALQAPVNHQHFDGYSMGASTQTK
                     YKSLEHIPGLAVGGWFDAGDFDIQTGSHCDAVLALVDAWEKFRPLRDETYIDYPTHYV
                     DIHRPDGKPDILQQIKHGTLNMVAQVKNIGHPVRGIVVPNLHQYHHLGDGSTETDNLP
                     YNPELKPYESDGRSSGTMDDRWAFTERTSFLDYYTAAALAAACRALRGFDDSLAEQSL
                     YYAQKLWNENDTVSKNDTSRFAAMFRRNLKTTAALQLYITTSDQRYAKEFRDDIWQTL
                     DRSVLFGMTTALQACAYMDDAYRQKLKEYVIKYKALSDGYTRQNPYGVPMAAGGWGGN
                     GGIINWAITNYYAHTVFPDIVGVEDVYRGLDYIFGCHPYSNLSFVSSVGTRSKKVTYG
                     NNRADFSYIAGGVVPGILIIKPDFPENKEDWPFLWGENEVTVGICANYIFLSAAAVSL
                     PGEK"
     gene            2956..>3890
                     /locus_tag="VKQ52_10545"
     CDS             2956..>3890
                     /locus_tag="VKQ52_10545"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HLZ87673.1"
                     /translation="MHSIYSRTIAAGMLTLLLLGSRSSYAQDPKFYIFLSFGQSNMEG
                     AAKPEAADTIVDPRFRVMEAVDCPDLKREKGHWYTAAPPLCRCHTGLTPADYFGRTLV
                     AHLPAGITVGVINVAVGGCKIELFDKDHFQSFTANAPDWMKGIIKEYDGNPYARLVEM
                     AKLAQKDGVIKGILLHQGESNTNDSTWPAKVKLVYGNLLSDLGLKAEDVPLLSGEVVN
                     ADQGGVCASMNGIIDALPHTIPTAHVISSAGCPAGNDHLHFTAAGYRELGRRYGVTML
                     SLLTPAQPMPPGFDSVRADIPHGRIDTISYASATVG"
BASE COUNT          957 a         1126 c         1036 g          771 t
ORIGIN      
        1 ccaccatcgc cgtcagcttt acggagaacg gcatccggcg aaccgtctac cgcgatgtca
       61 actccggcgg aagcttcggc gcctcgcccc tcagacagca gatcggcatc ggccgcgccg
      121 aaaagatcga caggctcgaa atcacctggc cgacaaccgg gctgatacag tcattttcga
      181 atatagcccc ccggcaattc atccacatca gggaaggtga aaacgaaata aaaacgatga
      241 acatcagggc gtttcccttc aaatgcctcg accttcaaaa gaatagcgcc gcttgcgaac
      301 ccccaatggt ggggataacc cattccgtca aataaaccta agtatgaata aactagcttt
      361 atccgcgatc ctcctttttg ggtttaccct ttttggcaag actcaaacca tcgcaaaaca
      421 tgccctccca tccctcgcaa aacatgccct cccatccccg acacaatcat tgacgatcaa
      481 cgactccggc tattttgaaa agcgcggggt caacctgctc gtcttcacca accagtataa
      541 tgggatgttc tacgacgaga agaccgccgg catcgaattg atccatcatg gcgtaaggac
      601 ctgcaccggc ggagccgtac gactccagaa tacgcctgaa cagtgggacc tcatccccat
      661 cctggtgagc agaaaagtag ataaagcaaa caacagcatc gaggtcgtcc tgaagtatag
      721 agaatacgac ttcctgtcga gaatccgcgt ctccgcaaaa gacagcggcg tagagatcag
      781 cgtttacctc gatcaacccc ttcctgaaaa actggaaggt aacgcggggt tcaacctgga
      841 attcctgccc gccgcctatt ttgaaaaaac ctacctcgcg gatggccggc ccggaacctt
      901 cccgttatat cccgccggcc agaccaaaat ggaaccgctg gctcagaaga tcccccaatt
      961 cgccggtcat accacctttg acgacagggg caggaacgag tttatcgtcc cgctgccgct
     1021 ggcgacggca agatccatgg tgctggcgcc ggaagacccc gcccgggaag tcaccatccg
     1081 gtcccaggac gccgatctga tgctcttcga cggacgcaac ctggcacaga acggctggtt
     1141 catcctgcgc agcctgctgc ccgcgaaaaa aacaggaaaa gtattgacct ggtacctgga
     1201 acccagcgcc atccccgact ggaagcggaa acccgtgatc ggtttctcgc aggtgggcta
     1261 tcgccccaat caggaaaaga cggcggttat cgagctggat aagaacgacg cgccattgcc
     1321 aaccgcttcc ctcttccagg tgacagccga gggtacaccc gtggagaaat ggaagggcag
     1381 cgtccattcc tggggaagat acctgcggta tacctatgcg aaattcgatt tcagcgccgt
     1441 taaagacccg ggattatatt atattcaata cggtcaggaa aggacgaaca ctttccggat
     1501 cagcccggat gtgctcgacg atagctggca tcctaccctt gatgtctggt tgccggtgca
     1561 aatggaccat atggaagtca atgaagccta ccgggtatgg cacggcgtac cctttatgga
     1621 cgacgctttg caggcgccgg tgaaccacca gcatttcgac ggctacagca tgggcgcgtc
     1681 cacccaaaca aaatacaagt ccctcgaaca catcccgggt ttagcagtag gcggatggtt
     1741 cgatgccggc gacttcgata tccaaaccgg ctcccattgc gatgccgtac tggccctcgt
     1801 ggatgcctgg gagaaattcc ggcctctccg ggacgagacc tacatcgact atcccaccca
     1861 ttatgtcgat atccaccggc cggatgggaa gcccgatatc cttcagcaga tcaaacatgg
     1921 aacattgaac atggtcgccc aggtaaaaaa catcggccat ccggtcaggg ggatcgtggt
     1981 gcccaatctg caccaatatc atcacctcgg agacggctcc accgaaacgg ataaccttcc
     2041 ttataatcct gaactaaaac catatgagtc cgatgggaga tccagcggca ccatggacga
     2101 ccgctgggcg ttcaccgaaa ggaccagctt cctggattat tatacggcag ccgccctggc
     2161 cgcggcctgc cgtgcactac ggggattcga cgacagcctc gcggaacaaa gcctttacta
     2221 cgctcaaaag ctgtggaatg agaacgatac ggtaagcaaa aacgatacct cacggtttgc
     2281 tgcgatgttc cggcgaaact tgaaaaccac cgccgccttg caattgtata taaccaccag
     2341 cgatcaacgc tatgcaaagg agttccggga cgatatctgg cagacactcg acaggtccgt
     2401 actgttcggc atgacgacgg cgctgcaggc ttgtgcgtat atggatgacg cgtacaggca
     2461 gaaactaaag gagtatgtca taaaatacaa ggccctgagc gacggctata cgcggcaaaa
     2521 tccctatggg gtaccgatgg ctgccggcgg ctggggcggg aatggcggca tcatcaattg
     2581 ggcgatcacc aactactacg cccataccgt cttccccgac attgtgggtg tggaggacgt
     2641 ttacaggggt ctcgactata ttttcggatg ccatccctat tcaaacctct ctttcgtctc
     2701 ttcagtcgga acgcgttcca aaaaggtgac ctatggtaac aaccgcgctg atttcagcta
     2761 tattgcaggc ggcgtcgttc cgggaatatt gatcattaaa ccggattttc ccgagaacaa
     2821 agaggactgg cccttcttat ggggagaaaa cgaagtgacg gtcggcatct gcgccaacta
     2881 tatctttctt tcggccgcgg ccgtttcgtt gccgggggag aaataacatc ggatcataaa
     2941 accagacatc gactaatgca tagtatttat tcaaggacca tcgcagccgg aatgctgacg
     3001 ctcctgctgc tgggaagcag gtcttcctac gcacaggatc ccaaatttta tattttcctg
     3061 agtttcgggc agtcgaatat ggaaggggct gccaaacccg aagccgcgga cacgatcgtc
     3121 gacccgcgtt tccgggtgat ggaggcggtg gattgcccgg acctgaagcg ggagaagggg
     3181 cattggtaca cggccgcgcc gccactgtgc cggtgtcata cgggcctgac gcctgcggac
     3241 tatttcggac gtacgctggt cgcgcacctg cccgccggta tcacggtcgg cgtgatcaat
     3301 gtagccgtcg gcgggtgtaa gatcgagctc ttcgataaag atcattttca atccttcacc
     3361 gccaacgccc cggactggat gaaagggatc ataaaggaat acgacggcaa tccctacgca
     3421 cggctggtgg agatggcgaa gctggcccaa aaggacgggg tcatcaaggg catcttattg
     3481 caccagggcg aatcgaacac caatgacagc acctggccgg ctaaggtgaa gctcgtttac
     3541 ggaaacctgt tgagcgacct gggactaaaa gccgaagacg ttcctttgtt gtcgggggaa
     3601 gtggtaaatg ccgaccaggg aggcgtttgc gcaagcatga acgggatcat cgatgcattg
     3661 ccgcacacga taccgacggc ccatgtcatc tcttccgccg gctgcccggc cggtaatgac
     3721 catttgcatt ttacggccgc cggataccgg gagctggggc gccgctatgg ggtaaccatg
     3781 ctttcgctgc tgaccccggc acagccgatg ccccccggtt tcgactcggt gcgggccgac
     3841 atcccgcatg gcaggatcga caccatcagc tatgcttccg ccacggtcgg
//
LOCUS       DATGFG010000562         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1144071, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000562 DATGFG010000000
VERSION     DATGFG010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144071"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VKQ52_10550"
     CDS             <1..1228
                     /locus_tag="VKQ52_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87674.1"
                     /translation="NVSINGVQVEYNPSAYVAFAAGTVDYRFRDYTVGDATKGQYLAL
                     ARYGWGKKDGNNVILTYYTGKRQLYNVNTTTQGTAVPDYNLMGFTLQGCYRLGKTTAF
                     IAEVAKSSLPYYSLDSSHAHSVLASALRMNDRSNEAYSVKVNSFIPRSQTQLSVTYSR
                     FGANFQSFSLFTTGTRQSAWALRVDQPFFQKHLSVTGSIRTNDFVNPLLSTAYSSTAV
                     FASIQATMRLKKWPTVSFGYFPSSQIVRLDNARYQENLFYTMTASVSHSYKTKGISMI
                     SLLLYTRFYNKITDTGFIYFNTQNLLLSQTVLLGRLSVQLQGSVAANASYTLYVADGK
                     AEYRIWNWMSLGAGIKYNAQTVYDIRQWGYSATAAMRVPKVGEFRLMGDKGFIPGNNR
                     RLVPNNLGRLTYSKVF"
     gene            1238..2341
                     /locus_tag="VKQ52_10555"
     CDS             1238..2341
                     /locus_tag="VKQ52_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87675.1"
                     /translation="MRKKISSALLVGLLAFVGTRAQVSMVLQVPPTGVMQKNQLWNMA
                     IVNAGATGSRVVIQLTLLDGKDGQAVMTAQTRPILLTSGTTMVTAREVAPVQYEYLSP
                     QFNIDRDPNGFLPVGNYKACYSILLQAQKSEGRMAEDCIPVEVQPLSPPQLNLPADTS
                     TVATAYPPFSWLPPTPVNLFSNLTYDFLLVEVLPDQGAYQAIQENIPTYTISHLKDPV
                     HLYPASAKPLDTGRIYAWRILALNEGQFIAQSEVWTFKLATAKAPLVVPGGHYMPMRA
                     VNERSAGIHVHTCDTIGIKFYSFEKDHPAVVNFYAADGTIVKTAQEHIVYGDNYLAYV
                     LNRAFEKGKVYRIELADTRKNIYSTSFILTTAK"
BASE COUNT          592 a          630 c          680 g          585 t
ORIGIN      
        1 gaatgtcagc attaacgggg tccaggtaga atacaaccca tcggcctatg tggcctttgc
       61 agcgggtacc gtggactacc ggttccggga ctataccgtg ggtgacgcca caaagggcca
      121 gtatcttgct cttgcccggt atggttgggg gaaaaaggac ggcaacaatg tcatcctcac
      181 ctactatacg ggcaaacgcc agttgtacaa tgtcaatacc accacgcagg gaactgcggt
      241 gccggattat aacctgatgg gctttaccct gcaaggttgt tatcgactcg gaaaaacgac
      301 ggcgttcatc gcggaagtgg ccaaatcatc gcttccttac tacagcctgg atagcagcca
      361 cgcccacagc gtcctggcca gcgccctgcg gatgaatgac aggagcaatg aggcctattc
      421 tgtaaaggtc aattccttta ttcccaggtc tcagacgcag ttgagcgtca cctattcgcg
      481 gtttggcgcc aactttcaat cgttcagtct tttcaccact ggtacgcggc agagcgcatg
      541 ggcgttgaga gtggaccagc cctttttcca aaagcacctg tcggtgacag gttcgatcag
      601 aaccaacgat tttgtcaatc ctttactcag tactgcgtat agcagtaccg cggtgtttgc
      661 gagtatccag gcgaccatgc ggctgaaaaa atggccgacc gtctccttcg gctattttcc
      721 ttcttcgcag atagtccggc tggacaacgc ccggtaccag gagaatcttt tctataccat
      781 gaccgccagc gtgagtcatt cttataagac aaagggcatt tcgatgatca gtcttttgtt
      841 gtacacacgg ttctacaata agattaccga caccggtttt atctatttca atacccagaa
      901 cctgttgcta agccagacgg tgttgctggg gcggttgagt gttcagttgc agggttcggt
      961 ggcggccaat gcttcctata ccctttatgt tgccgatgga aaggcggaat accgcatttg
     1021 gaattggatg tcacttggcg cggggataaa gtacaatgcc cagaccgtct atgacatccg
     1081 gcaatgggga tattcggcga ccgcggccat gagggtgcca aaggttggcg aattccggct
     1141 gatgggcgac aaaggattca tcccgggcaa taacaggagg cttgtgccca acaacctggg
     1201 acgattaacc tatagtaaag tattttaaaa gctgtctatg cgaaaaaaaa tcagttcggc
     1261 gttgcttgtg ggtctgctgg cttttgtcgg gacgagggcg caggtttcca tggtgttaca
     1321 ggtgcccccc accggcgtga tgcagaaaaa tcagctgtgg aatatggcca ttgtcaatgc
     1381 gggtgcgacg ggatcgagag tggtgatcca gttgaccttg ctggacggca aggacgggca
     1441 ggcagttatg acggcgcaga cccgcccgat attgttgacg agcggaacga cgatggtcac
     1501 cgcaagggag gttgccccgg tgcaatacga atatctttcg ccgcagttca acatcgacag
     1561 ggatccaaac gggttcttgc cggtaggcaa ttacaaagcc tgttattcga tactacttca
     1621 ggcgcaaaaa agcgaaggca ggatggcgga agattgtatt cccgttgaag tgcagcccct
     1681 gagtccgccg caattgaatt taccggcgga tacgtcgacc gtagcgacgg cctatccccc
     1741 tttcagctgg ttgccaccga cacccgtcaa tcttttcagt aaccttacgt atgacttcct
     1801 gctcgtggag gtattgcccg atcaaggcgc ctaccaggcc attcaggaga atatcccgac
     1861 ctataccatc agtcatctga aggaccctgt gcacctgtat ccggcgtccg caaagccgct
     1921 ggataccggc aggatatacg cgtggcggat ccttgcgcta aacgaaggac agttcatcgc
     1981 gcaatcggag gtctggacct tcaaattggc gacagccaag gcgccgctgg tagtgcccgg
     2041 ggggcactat atgccgatga gggccgtcaa cgagcggtcg gcgggtatcc atgtccacac
     2101 ctgtgatact atcggcatta aattctattc cttcgaaaag gatcatccgg ccgtagtaaa
     2161 tttttacgct gccgatggaa cgatcgtgaa aacagcgcag gagcatatcg tgtatggtga
     2221 caattacctg gcgtatgtgc tgaacagagc ctttgagaaa gggaaagtgt accgcatcga
     2281 gcttgccgac accaggaaaa atatctattc gaccagtttt attttaacca cggccaaata
     2341 acagagcaaa agatatgatc ttactactag ctacccggta tcgaaaatgt attgctttcc
     2401 tgctcttctt tgtcgcgggt ctctttcacg atgcagcagc gatggccaac gaagcggggc
     2461 tgaggggggc gtttgtgcct gtttcgt
//
LOCUS       DATGFG010000563         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1533061, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000563 DATGFG010000000
VERSION     DATGFG010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533061"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..508
                     /gene="uraH"
                     /locus_tag="VKQ52_10560"
     CDS             191..508
                     /gene="uraH"
                     /locus_tag="VKQ52_10560"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HLZ87676.1"
                     /translation="MSTITTHILDISQGRPASGVEVILYTTNCEITRSRTNSDGRITG
                     WDANVGPGVYTLRFETKHWYESQSIPTFYPYVEIHFEITAVAHYHIPLLISPWGYSTY
                     RGS"
     gene            517..1953
                     /locus_tag="VKQ52_10565"
     CDS             517..1953
                     /locus_tag="VKQ52_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87677.1"
                     /translation="MMKISIDEKDKESLLASLGAANLAFQHNYPGDRPDRQPVHTVYG
                     GANLFKSDTCPKMGDTALRSLRTYASNFAELARALSLEGHELLPHTEADILALDKKLQ
                     GLPEDQRRKEKAWLPWAVYHKVIKKLTAEPVEDFRIDFEDGYGNRPDEEEDATAITAA
                     RELAAGMRNNTLSPFIGIRIKPFTEDLKTRSVRTLDLFLTELLEQTGGALPANFIVML
                     PKVTIPEQVTTLVRLFRLLEKANRLPDNTLKMETMVEATQIIMDEDGRNPLYRIIKAS
                     EGRCIAAHFGTYDYTASCGITARYQTMAHPVCDFAHHMTKVALAGTGIWLSDGATNIM
                     PIGPHRGEHLSFEQQQENRAAVHRAWRTGFGHTMHSLINGFYQGWDLNPAQLPMRYAA
                     TYTFFLSSLDDAIHRLKIFVERSAVSTLTGDIFDDAATGQGLLNFFLKAMNCGAIEEK
                     EALATGLTLDEIRSRSFLQILKGRRGQG"
BASE COUNT          500 a          705 c          454 g          347 t
ORIGIN      
        1 gaaaaattcg gctatatcta tatcgtttgc gccaccggcc gatcggcagc cgaaatgctc
       61 ggcattctca acacccgcct caacaacacc ccggagcagg aaattcacat agccatgacc
      121 gaacaggaaa agatcacccg cctccgcctc caaaaattac tcacatgagc accatcacaa
      181 aattattccc atgagcacca tcaccaccca catcctcgac atctcccagg gccgtcccgc
      241 atccggcgtg gaagtcatcc tctataccac aaactgcgag atcacccgca gccgcaccaa
      301 cagcgacggc cgcatcaccg gctgggacgc gaacgtcggc cccggtgtct atacgctccg
      361 gttcgagaca aaacactggt acgagtccca gtccatcccg accttctacc cctacgtgga
      421 gatccacttc gagataaccg cggtcgccca ttaccatatt cctttactga tcagcccctg
      481 gggctattcc acctaccggg gatcctaaaa cctgacatga tgaaaatatc gatcgacgaa
      541 aaagataaag aaagtctcct ggcctccctg ggagcggcta accttgcctt ccagcacaac
      601 taccccggtg accggcccga ccgtcagccc gtacacaccg tctacggtgg cgccaatctt
      661 ttcaaaagcg acacctgccc aaaaatgggc gacaccgccc tgagaagtct ccgcacctac
      721 gcctccaact tcgccgaact ggcccgcgcc ctctcgctcg aaggtcacga acttcttccc
      781 catacggaag ccgacatcct ggccctggac aaaaaactgc aaggccttcc cgaagaccag
      841 cgccggaaag aaaaagcctg gcttccctgg gccgtctatc ataaggtgat caaaaagctg
      901 acagccgagc ctgtcgaaga cttccgcatc gacttcgagg acggctatgg caaccgcccc
      961 gatgaagaag aggacgccac cgccatcacg gccgcgcggg aactggccgc cggtatgcga
     1021 aacaataccc tgtccccctt catcggcatc cgcataaaac cctttaccga agacctcaaa
     1081 acccgcagcg tccgaaccct cgacctcttc ctcaccgaac tcctggaaca aaccggcggc
     1141 gcgcttcccg ccaacttcat cgtcatgctg ccaaaagtga cgatccccga acaggtgacc
     1201 accctcgtcc ggctcttccg cctcctcgag aaagcaaacc gcctccccga caataccctg
     1261 aagatggaga cgatggtaga ggctacccag atcatcatgg acgaggatgg gcgcaacccg
     1321 ctttaccgga tcataaaagc ctccgaaggc cgctgtatcg cggcccattt cggaacctat
     1381 gactacacgg cctcctgcgg catcaccgcc cgctaccaga ccatggccca cccggtctgc
     1441 gatttcgccc accacatgac aaaggtcgcc ctcgccggca ccggcatctg gctctccgat
     1501 ggcgccacca acatcatgcc catcggtccg catcgtggcg aacacctcag cttcgaacaa
     1561 caacaggaga accgcgccgc cgtgcaccgc gcctggcgca ccggctttgg ccacaccatg
     1621 cactcgctga taaacggctt ctaccagggc tgggacctca accccgcaca acttccgatg
     1681 cgctacgccg ccacctatac cttcttcctc agcagcctcg acgacgcgat ccacaggctg
     1741 aagatctttg tcgaacgcag cgccgtttct acgctcacag gggatatctt cgacgacgca
     1801 gcgacaggac agggactgct caatttcttc ctgaaggcaa tgaactgcgg cgccatcgaa
     1861 gaaaaagaag cgctggcgac aggcctcacg ctcgacgaga tccgcagtcg cagcttcctt
     1921 cagattttaa aaggcagaag agggcaggga tagtgttttt ttgtaccttc cacctatatg
     1981 catttatcac caaaagaaac ggagaa
//
LOCUS       DATGFG010000564         5700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_20027, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000564 DATGFG010000000
VERSION     DATGFG010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20027"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VKQ52_10570"
     CDS             complement(<1..905)
                     /locus_tag="VKQ52_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLZ87678.1"
                     /translation="MKSLLIRGFLFALALVFGGEARAGGADNDTALLRQMIVTYFGAV
                     ADKDFVKLTAMTTEDFTIYEIGKVWNNDSVFRNIQYHQPFTVKFTLTDFKVVADARLA
                     FGSYHSHAAFVIADSIKFTLDFIETATFRKTAAGWKINLIHVTSLEPPVVDMPRLYRR
                     YDTVRYVPEHYRERVELFGSEAPGKGGTIFLGNSITEFGDWKRLLHDPSVLNRGIAGD
                     NTFGILDRLPEVIARQPAKLFIEAGVNDIGQGVPVGMIVGNISSIVGYVRVKSPGTKI
                     YVVSVLPTNENARSEYPEVCGKNAVV"
     gene            complement(951..1862)
                     /locus_tag="VKQ52_10575"
     CDS             complement(951..1862)
                     /locus_tag="VKQ52_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ87679.1"
                     /translation="MKRPVKPYIELESIGELHELIRCSPPRHPLISIIDHTDFYALRP
                     KQDVLYRFGFYTISCKKFKGLLNYGKSRYDFKEGSLLFTAPGQVIGAGPDVTVDEGWA
                     LFVHPDLLHGTSLGSRMHQYSFFHYEVNEALHISEEEKAIIKDCVDKIARECRQPIDK
                     HTQGLLVSNIELLLNYCNRFYDRQFYTRTRTNADVVQRFEVLLKEYFSQGNLIETGLP
                     AVTHFASKLHLSPNYLSDLLQKSTGKSTLEHIHLELVEKAKSLLWGTDNPISEIAYEL
                     GFEHPSHFTKIFKAKTGKSPSEYRVRN"
     gene            complement(1890..2702)
                     /locus_tag="VKQ52_10580"
     CDS             complement(1890..2702)
                     /locus_tag="VKQ52_10580"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ87680.1"
                     /translation="MKKTVFITGASSGFGRATVQLFHENGWNVIATMRTPENEKSLST
                     PGGVLLTRLDVTDKSSIGAAVQAGMRQFGQIDVLVNNAGFGSLGALEAAPEELIRQQF
                     DVNFFGLIEVTKAVLPQMRARRSGVIINVSSIGGRLGFPFSTLYHATKFAVEGLTESL
                     QYELNPLGIRLKLVEPGGYKTDFLGRSMGFYGVGGLDDYRASFDRFNDKLSQWPLSEN
                     IGEVAGAIYEAATDGSEKLRYPIGQDALPLLEARQQMDDIAFKKLMVGETGI"
     gene            2822..3844
                     /locus_tag="VKQ52_10585"
     CDS             2822..3844
                     /locus_tag="VKQ52_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLZ87681.1"
                     /translation="MRYKLLGRSGLKVSELCLGTMGFGTEAGWGADKAASFAIMEAFA
                     NAGGNFLDTANIYKLGTSEKIIGEFITPRDRDFFVLATKYSLKDNTTNPNASGNNRKN
                     MMRSVERSLKRLQTDFIDLLYLHIWDDLTPIDEVLRGIDDLIRQGKVNYAAISDTPAW
                     IVSKGNTMAELMGWSQFVALQVEYSLLQRTPERELTPMAKHYGMTVTPWAPLAGGALT
                     GKYLRGEQGRVKPESNRRDENSTRITREVMAIAGKLGVEPSQVALQWTRQQGFECIPI
                     VGATRLEQLHENLQMVDLVLAPDDLKRLDEASAIDLGFPGKFFREEGVRQNNFGGFHD
                     RIIRRS"
     gene            complement(3860..4714)
                     /locus_tag="VKQ52_10590"
     CDS             complement(3860..4714)
                     /locus_tag="VKQ52_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HLZ87682.1"
                     /translation="MKNTFTMHACLGVLLAIFVSGCKTVNPGEVGFVVHKGVIQPGIL
                     SPGRYHVNIFSSRIHTFKTRINEYSTIMSPPTKEGLEVKVNLTVLYHIKPEAAPTIYT
                     NVGMDYGNTIVINNFMAIVREYTMTYTAIDLLGERETIEKNIEDKLRAAISPYGIVLD
                     DVLVKDIDMPSQVLEAIENKAKADQVAKQTTLELQTKRERENFDLETREKELKFSLDK
                     QRNDSLMMQIEANAVRNYQNIINPSLTDRLLKFKSIEVTKELVKSPNAKVIITDGKSM
                     MLNTIGEK"
     gene            complement(4838..>5700)
                     /locus_tag="VKQ52_10595"
     CDS             complement(4838..>5700)
                     /locus_tag="VKQ52_10595"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLZ87683.1"
                     /translation="LYLLSDVIYGILYYLIGYRRKVVMDNLRQAFPEKSEAERIRIAK
                     KFYHNFIDSFVEVIKLVSANESWLHKRFTVDARVLEEVYATGKPCQMHLGHTFNWEWG
                     QIVLTALTKYQILVVYMPIGNKVFEKLFYRLRTRSGNVFLPAPNMREAIVPYLPTQYL
                     LGLVADQCPGNMQTSYWIDFLGRPTPFASGPEKGARYGHLPVVFAAIDKPRRGHYHAT
                     IKLACMDAAELPEGELTREYVRYLESVIRQRPDMWLWSHRRWKHAWKEAYEPMRIDEG
                     RQAPWSAPVS"
BASE COUNT         1356 a         1686 c         1383 g         1275 t
ORIGIN      
        1 accaccgcat tcttcccaca tacctccggg tattccgacc gggcattttc attcgtcggt
       61 aacacactga cgacatagat cttcgttccg ggcgacttca cccgcacata acccacgatc
      121 gaggagatat tccccacgat catcccaacc gggacaccct gcccgatatc gttgaccccc
      181 gcctcgataa agagcttcgc cggttgacgg gcgatcacct cgggcaaacg gtccaggatc
      241 ccgaaggtat tatcccccgc aatcccgcgg ttcaacacgg aaggatcatg caaaagccgc
      301 ttccagtccc cgaattccgt gatactgttg cccaggaata tcgtcccgcc cttccccggc
      361 gcctcgctcc cgaacaactc caccctctcc cggtaatgtt ccggtacata ccgcacggtg
      421 tcatatcgcc ggtacagcct gggcatatcc accaccggcg gttccagcga cgtcacatga
      481 atgaggttga tcttccagcc cgccgccgtc ttccggaagg tcgccgtctc gatgaaatcc
      541 agggtgaatt tgatcgaatc ggcaattaca aaagccgcat ggctgtgata gctgccaaag
      601 gccaaccggg catccgcaac cactttaaaa tcagtaaggg tgaatttgac ggtgaaaggt
      661 tgatgatatt ggatgttccg gaacacactg tcgttattcc agacttttcc aatctcatag
      721 atggtaaagt cttcggtcgt catcgctgtc agcttcacaa aatccttatc cgccaccgcg
      781 ccgaaatagg tgacgatcat ttgccgcaac agggccgtgt cattatccgc ccccccggcc
      841 cgcgcctcgc ccccaaaaac caacgccaat gcaaacaaaa aaccacgtat aagtagactt
      901 ttcatacccc aatttacccc atcccctccg gcaccgatat gccaaaatgc tcaatttctt
      961 actcggtact cactcggcga ctttcccgtc ttggccttaa agatcttggt aaaatgcgaa
     1021 ggatgctcaa aaccgagctc ataggcgatc tcactgatcg ggttatctgt cccccataac
     1081 aacgacttcg ccttctcgac aagctcgaga tggatatgct ccagcgtgct cttcccggtg
     1141 gacttctgaa gcagatcgga aaggtaattg ggcgacagat gcagcttcga cgcaaaatgc
     1201 gtaacggcag gcagcccggt ttctattaaa ttgccctggc tgaagtattc tttcagcaac
     1261 acctcgaagc gttgcacgac atcggcattg gttcttgtcc gggtatagaa ttgccggtca
     1321 taaaaccggt tgcagtagtt cagcagcagt tctatattgc tgacaagcag gccctgggta
     1381 tgcttatcga tgggttggcg gcattcccgg gcgatcttat cgacacaatc cttgatgata
     1441 gccttttcct cctccgagat atgcagcgcc tcattcacct catagtgaaa aaaggaatac
     1501 tggtgcatcc tgctgcccag gctggttcca tgcagcaggt cggggtgaac gaacaaagcc
     1561 catccttcat ctaccgttac atcgggaccc gcgccgatca cctggcccgg tgcggtaaaa
     1621 agaagcgacc cctccttgaa atcataacgg cttttcccat agttcaacag ccccttgaac
     1681 ttcttacagg agatcgtata gaaaccaaac cggtacagga catcctgttt cggccgcagg
     1741 gcataaaaat ccgtatggtc gatgatgctg atcagggggt gacgcggcgg ggaacacctt
     1801 atcagttcat gcaattcacc gatagattca agctcgatat aaggcttaac tggccgtttc
     1861 attggttata taaagatacg cccgataatt cagatcccgg tctcgccgac catcagcttc
     1921 ttgaacgcaa tatcatccat ttgttgccgc gcctccaaca aaggcaaggc atcctggccg
     1981 atgggatagc gtaatttctc gcttccgtcc gtggcggcct cgtaaatggc cccggccacc
     2041 tcgccgatat tttcagacaa cggccactgc gatagtttgt cattaaaccg gtcaaaggaa
     2101 gcccgatagt catccaggcc gccgacgcca taaaacccca tcgagcgtcc gaggaaatcg
     2161 gttttatacc cacccggttc aaccagcttc aaccggatgc ccagcgggtt gagttcgtat
     2221 tgcagggact cggtcaaccc ctccaccgcg aacttggtgg catggtaaag cgtcgagaac
     2281 gggaacccga gcctcccgcc gatggaagac acattgatga tgacaccgga tctgcgcgcc
     2341 cgcatctgcg gtaaaacagc cttggtcact tcgatcaggc cgaaaaagtt cacgtcgaac
     2401 tgctgacgga tcaactcctc cggtgccgct tccagcgcgc caagcgaccc aaagccggca
     2461 ttgttcacca gcacatcgat ctgcccgaat tgacgcatcc cggcctgaac agcagccccg
     2521 atgctggatt tatcggtaac atccagccgg gtcaggagca cgccgccggg cgtggaaaga
     2581 ctcttttcat tttcgggagt acgcatagtg gcgatcacat tccagccatt ctcatggaat
     2641 aactgcacgg ttgcccttcc gaaaccggag gaggcgcctg taataaatac tgtctttttc
     2701 atttgagctt gtattgattg tgacaacaaa gctcccaaca actccccggc tctacttata
     2761 tgtttcttcg gaaggcttat acatttctta gtaccttcgc ccctcagcac aattttcacc
     2821 catgagatac aaattactcg gacgcagcgg cctcaaagta tctgaactat gcctgggaac
     2881 aatgggcttc ggcaccgagg ccggctgggg cgccgataag gcggccagct tcgccatcat
     2941 ggaagccttc gccaatgccg gcggcaattt cctggacacg gccaacatct acaaattggg
     3001 cacaagcgaa aagatcatcg gcgaattcat caccccgcgc gaccgcgatt tttttgtact
     3061 ggccaccaaa tattcgctga aggataatac caccaacccc aatgccagcg gcaacaaccg
     3121 gaaaaatatg atgcgcagcg tcgagcgaag tctcaaacgg ctgcaaaccg acttcatcga
     3181 tctgctgtac ctgcacatct gggatgacct cacccccatc gacgaggtgt tgcggggtat
     3241 cgacgatctt attcgccagg gcaaggtcaa ctatgccgcc atcagcgata cgccggcctg
     3301 gatcgtaagc aaggggaata ccatggccga actcatgggc tggagccagt tcgtggcgct
     3361 ccaggtagaa tacagcctcc tccagcgcac gcccgaacgg gaattgaccc ccatggccaa
     3421 acactacggg atgaccgtaa caccctgggc ccctttagcc ggcggcgccc tcaccggtaa
     3481 atacctgcgc ggtgagcagg ggcgggtgaa gccggaaagc aaccgccgcg acgagaacag
     3541 cacccgcatc acccgcgaag taatggccat tgccggcaaa ctgggcgtcg agcccagcca
     3601 ggtggcgctg caatggaccc gccagcaggg tttcgaatgc atccccatcg tcggtgccac
     3661 ccgcctcgaa caactgcacg aaaacctcca aatggtcgat ctcgtgctgg ctcccgacga
     3721 cctcaaacga ctggacgaag ccagcgccat cgacctcggc ttcccgggca aattcttccg
     3781 ggaagagggc gtccgccaaa acaatttcgg cggcttccac gaccggatca tccggcggtc
     3841 atagctttcc cctggccccc tatttctcgc cgatagtatt cagcatcatc gatttcccat
     3901 cggtaatgat caccttggca ttgggtgatt tcacgagctc ttttgtcacc tcgatgctct
     3961 tgaatttcaa caaccggtcc gtaagggagg ggttgatgat attttgataa ttgcggacgg
     4021 cattggcttc gatctgcatc atcagagaat catttctttg cttgtccagc gagaacttca
     4081 attccttttc ccgggtctcg aggtcgaaat tctcacgctc ccgtttcgtc tgcaattcaa
     4141 gggtggtctg cttggccacc tggtctgcct ttgccttgtt ctcgatggcc tccaacacct
     4201 gcgaaggcat gtcgatatcc ttcaccagca catcgtccag cacgatccca taaggagaga
     4261 tggccgctct caatttatcc tcgatattct tctcgatcgt ttcccgctcg ccaagcaggt
     4321 cgatcgccgt ataggtcatg gtatactctc tgacgatggc cataaaatta ttgatgacaa
     4381 tggtgttgcc ataatccatg ccgacattgg tgtaaatagt gggcgcggcc tcgggcttga
     4441 tatggtatag gaccgtcagg ttcaccttca cttccagccc ttccttggtc ggcgggctca
     4501 taatggtgga gtactcgttt atccgggtct taaacgtgtg gatcctggac gagaagatat
     4561 tgacatgata ccgccccggc gacaagatac ccggttgtat cactcccttg tgaacgacaa
     4621 acccgacttc cccgggatta acggtcttgc aaccggagac gaaaatagcc agcaacacgc
     4681 ccaggcaggc gtgcatagta aaggtgttct tcataacaac agttttaagt tatagtaaaa
     4741 agcaacgaac cgaaggtata cccgcctccg gttaacccgt ggttaaaaga gtacctgcat
     4801 ttcctaaccg ttagttaaag aagcttattc ctatatccta agaaactggc gctgaccatg
     4861 gagcctgtct cccctcatcg atcctcatcg gttcatacgc ttccttccag gcatgtttcc
     4921 atctccggtg gctccacagc cacatatccg gccgttgacg gatcactgat tccaggtatc
     4981 gtacatactc gcgggtcagc tcgccttccg gcagctcggc ggcatccata caggccagct
     5041 tgatggtagc atgataatga ccgcgccggg gtttgtcgat agcggcaaac accaccggca
     5101 ggtgcccata gcgggctcct ttctccggtc cgctggcaaa aggggtgggg cgccccagga
     5161 agtcgatcca ataagaggtt tgcatattgc ccgggcattg gtcggcgaca agccccagca
     5221 ggtattgagt ggggagatac ggaacaatgg cttcacgcat attgggcgcc ggcaggaaaa
     5281 cattgccgct gcgggtgcga agccggtaga aaagcttttc aaacacctta ttgccgatcg
     5341 gcatatatac gaccagtatc tggtatttcg tcagcgcggt cagtacgatc tgaccccatt
     5401 cccaattgaa agtatgcccc aggtgcatct ggcagggctt gcccgtcgcg tacacttctt
     5461 ccagcacgcg cgcgtccacc gtaaatcgtt tgtgcaacca ggactcgttc gcggacacca
     5521 gcttgatgac ctccacaaaa gaatcgataa agttatgata gaattttttc gcgatccgga
     5581 tccgctcagc ctcgcttttt tcaggaaaag cctgccgcag attgtccatt acaaccttgc
     5641 gccggtaacc gataagataa tagaggatcc cataaatgac gtccgacaac aaataaagga
//
LOCUS       DATGFG010000565         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1301366, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000565 DATGFG010000000
VERSION     DATGFG010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301366"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /gene="tyrS"
                     /locus_tag="VKQ52_10600"
     CDS             complement(<1..981)
                     /gene="tyrS"
                     /locus_tag="VKQ52_10600"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HLZ87684.1"
                     /translation="MNLIEELKWRGMIQDIMPGTEEQLNKEMTSAYIGFDPTSDSLHI
                     GSLVPILLLVHLQRAGHKPFALVGGATGMVGDPSGKSEERNLLSEDVLNHNLACVKKQ
                     LEKFLDFNPGLPNKAEMVNNYDWFRDMRFLDFIRDVGKHITVNYMSAKDSVKKRLEGE
                     NGMSFTEFTYQLVQGYDFYWLYTNKNCKLQMGGSDQWGNITTGTELIRRKAGGEAFAF
                     TCPLLTKADGGKFGKTEKGNVWLDPEKTTPYQFYQFWLNATDDDARKWIKIFTFLSAG
                     EIDPLVHDHTARPEARLLQKALAQHVTTFVHGEEGYKKALADTEKMFANQS"
     gene            1195..2328
                     /locus_tag="VKQ52_10605"
     CDS             1195..2328
                     /locus_tag="VKQ52_10605"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HLZ87685.1"
                     /translation="MLKRMRGRKTLCSFLLVVAGSLLHLSAFAQFTKYSNEFLNIGAG
                     ARGLAMASAQVATVSDATAGYWNPAALANIRDNPQLSLMHAEYYAGIGKYDFASLALP
                     LKDNKRVLGLTALRFAVDDIPNTIFLVQPDGTVNFDNITTFSSADYAFIVSLAQREKI
                     FGDKTINFGVNAKIIHRTAGSFANAWGFGFDAATQITGKRWKLGVVAKDVTTTFNAWS
                     FSLDQAIREVFYETQNEIPGKSIELTAPQLLIGGSYNFRLGRKLNLLAEGDLDVTFDG
                     RRNTVISTNPVSIDPRLGLELSFKDVFFVRGGVNNFQRVLDDRDTLNKKKVWIYQPSV
                     GAGFKVGDIQIDYAFVNLANQSYPLYTHVFSLRIDLRRKEKKR"
     gene            2352..>2626
                     /locus_tag="VKQ52_10610"
     CDS             2352..>2626
                     /locus_tag="VKQ52_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87686.1"
                     /translation="MKKVFTFFFLLFTLGVAAQSYNNEWIDYSKTYYKFKVAGNGLYR
                     IPQATLVNAGLGGVPAQNFQLFRNGQEVPIYVSNGTGIMGGGDYIEF"
BASE COUNT          549 a          625 c          768 g          684 t
ORIGIN      
        1 ggattggttg gcgaacatct tttcggtgtc cgcgagcgcc tttttatagc cttcttctcc
       61 atgaacaaag gtggtgacgt gttgggcgag cgctttttgc agcagccggg cttcgggccg
      121 ggcggtgtgg tcatggacca gggggtcgat ctcgccggcg gataagaagg tgaagatctt
      181 tatccatttc cgggcgtcgt cgtcggtggc gttgagccag aactggtaga actggtaggg
      241 ggtggtcttt tccgggtcga gccagacatt ccctttttcc gtcttcccga acttgccgcc
      301 gtcggccttg gtcaggaggg ggcaggtgaa ggcaaaggcc tcgcccccgg ctttgcggcg
      361 gatcagttcg gtgccggtgg taatattgcc ccactggtcg cttccgccca tctggagctt
      421 gcaattttta ttggtgtata gccagtaaaa atcatagccc tggaccaatt ggtaggtaaa
      481 ttccgtgaaa ctcatcccat tttccccttc cagacgcttc tttacgctgt cctttgcaga
      541 catgtaattt accgtaatat gtttgcccac gtcgcggata aagtccagga agcgcatgtc
      601 cctgaaccaa tcgtagttgt tgaccatttc ggccttgttg ggcaggccgg ggttgaagtc
      661 gaggaatttt tccagttgtt tttttacgca ggcgagattg tggttgagga cgtcttcgct
      721 cagcaggttt cgttcctcgc ttttcccgct ggggtcgccg accatgccgg tagcgccgcc
      781 aaccagcgca aatggcttat ggccagccct ttgcaggtgt acgagcagga ggatgggcac
      841 caggctgccg atgtggaggc tgtcggatgt agggtcgaat ccgatgtagg cggaggtcat
      901 ttccttattg agctgttctt ccgttccggg catgatatcc tggatcatgc cccgccattt
      961 tagttcttcg atcaggttca tagtattttc gcgaatgggc ccgtggccca ttttagcgca
     1021 aaactaaagg attcccccca acttggggac aggtatgtta atagtacaat attgcgcagg
     1081 cggacgcgtt ataaatcaat gatccgtacc gttgaatcga agatccatca gactgctgag
     1141 aagacttcga tgtctgatga aagccaagag tataaatacc ttaaccgatg gcttatgctg
     1201 aaaagaatgc ggggcaggaa aaccctctgc tcctttttac tggtggttgc cggatccctt
     1261 ttacacctgt ctgcttttgc ccagtttacg aaatattcca atgaattttt aaatatcggc
     1321 gcgggggcgc gtggccttgc catggccagt gctcaggtgg ctacggtatc ggatgccacc
     1381 gcgggttact ggaatcctgc cgcgctggcg aatatccggg ataatcccca gctcagcctg
     1441 atgcatgccg agtactatgc ggggatcggc aaatatgatt ttgcgagcct ggcgctgccg
     1501 ctgaaagata acaagcgggt gctggggctt accgcgttgc ggtttgccgt cgatgatatt
     1561 cccaacacga tctttcttgt gcagccggac ggtacggtga atttcgacaa tatcaccacc
     1621 ttctcgtcgg ccgactatgc gtttatcgtg agcctggcgc agcgggagaa gatctttggt
     1681 gacaagacga tcaattttgg cgtgaatgcc aagatcatcc atcgtacggc ggggagtttt
     1741 gccaatgcct gggggtttgg gttcgacgcg gccacgcaga taacgggtaa acggtggaag
     1801 ctaggtgttg tggccaagga tgtaacgacc acgttcaatg cctggtcgtt cagtctcgac
     1861 caggcgatcc gggaggtgtt ttatgagacg cagaatgaga tccccgggaa atcgatcgaa
     1921 ttgaccgctc ctcagctgct gatcggcggc tcttataatt tccggttggg gaggaaactg
     1981 aatctgctgg cggagggcga cctggatgtg acctttgacg gcaggcgcaa tacggtgatc
     2041 agcacgaacc ctgtgagtat cgatccgcgg ctggggcttg aactttcctt taaggatgtg
     2101 ttctttgtcc ggggcggggt caacaatttc cagcgggtgc tggatgaccg ggatacgctg
     2161 aacaagaaga aggtctggat ctaccagccg agcgtgggcg ccggtttcaa ggtgggggat
     2221 atccagatcg attatgcgtt tgtgaacctg gcaaaccaat cctatccgtt gtatacccat
     2281 gttttttcac tacgaatcga tttgcggaga aaggagaaga agaggtgaca gaacagcaaa
     2341 accctattgg tatgaaaaaa gtatttactt tctttttctt attgtttact ttgggggttg
     2401 ccgcccagtc gtacaataac gaatggatcg actacagcaa gacctattat aagttcaagg
     2461 tcgccggtaa cggtctttat cgtatcccgc aggcgaccct tgtgaatgcc gggttgggcg
     2521 gggtgcctgc gcaaaatttc cagcttttcc gcaatggcca ggaagtgcct atttatgtga
     2581 gtaacggcac cgggatcatg ggtggcggcg actatatcga gttttg
//
LOCUS       DATGFG010000566         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1657544, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000566 DATGFG010000000
VERSION     DATGFG010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657544"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VKQ52_10615"
     CDS             <1..244
                     /locus_tag="VKQ52_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87687.1"
                     /translation="NLASDAGGKSNAAPASNALRQQDAAPPKAPGLQPWVSSFTYGFV
                     LSHGKSGISITQNVSSSTLRKLYCHDVGNVSLYFGW"
     gene            324..905
                     /locus_tag="VKQ52_10620"
     CDS             324..905
                     /locus_tag="VKQ52_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87688.1"
                     /translation="MKTTRKIGVVTALLVSFAFTSQAQTANPGNGNTGSSDKEWRISV
                     GPEAGLPLGNYSNGYNWYFGGSAQLDIPVMHSLFVTVNAGYDNLFAKSGEIPGTNLQL
                     IPLKAGLKYFFIGDLVYVQAQAGATIVANKTDVLADKSAAFTYTPQVGVLLKLAPKNY
                     IDVGFRWEQTSSFYNGGSNLSTLGLRVAYTFGL"
     gene            1132..1662
                     /locus_tag="VKQ52_10625"
     CDS             1132..1662
                     /locus_tag="VKQ52_10625"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ87689.1"
                     /translation="MATKWVLDPSHSEVEFKVKHMMISNVSGKFTKFDAAVETEGEDF
                     MTAKVTFTADVDSISTGAEQRDGHLKSPDFFDAAQFPQLKFVASKYENVDNDGSYEVW
                     GDLTIHGVTKNVKLDAEFGGVIKDPWGNTRAGITASGKINRKDFGLSWHGVTEAGSLI
                     VSDEVRIHVGLEFVKA"
     gene            1726..>3340
                     /locus_tag="VKQ52_10630"
     CDS             1726..>3340
                     /locus_tag="VKQ52_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HLZ87690.1"
                     /translation="MHYVSVEGLSKSYGVKPLFQQITFHIEEGDKIALVARNGSGKST
                     LLRILSGKDTAENGTVWIHKDVTVALFEQEPAFIEDRSILDNIFHHDHPVINAIKAYE
                     AASDEEDADKITDAIVRLDELGAWDFDTKVKQILGKLNIHHLQQPAGTLSGGQRKRVA
                     LAKTLIDIGFEHKHVLLIMDEPTNHLDVEMVEWLEHYLNQEKVTLLLVTHDRYFLDNV
                     TDEIWEMDGSSLHVYKGDYENYLEKKAARLENDTSSIEKARNTYRKELEWMRKQPKAR
                     TTKSKSRQDNFYVVEAKAKQRIEDQQVELQMKMNRLGGKVAEFKKVYKNYGEKVILKG
                     FDYTFKRGERVGVIGRNGVGKSTFINILQGLEPADSGKVNIGETVIFGNYSQQGLVIK
                     EDMRVIEFVKNIAEHFPLASGGSLSAAQFLNLFLFPPEQQYTYISKLSGGEKRRLHLL
                     SILFRNPNFLILDEPTNDLDLPTLAILENFLSEYQGCLLIVSHDRYFMDRLVDHLLVL
                     EGDGLIRDFPGNYSQYRDWLKDNEEDAAFP"
BASE COUNT          841 a          923 c          854 g          722 t
ORIGIN      
        1 caacctggca tccgatgcag gcggcaagag caacgccgca ccggcgtcca atgcgctccg
       61 gcagcaggat gcggcgccgc caaaagcgcc cggtctgcaa ccatgggttt cctcctttac
      121 ctatgggttt gtattatccc atggtaagtc tggtatttcc atcacgcaaa atgtttcttc
      181 ttccacattg agaaagctct actgtcatga tgtgggaaat gtctcgcttt atttcggttg
      241 gtgaataagt tttacgcgcg cgtccgaact tttttttaac agaatagtaa tagtaagtat
      301 tcgtaaacat aaatcaaaca aagatgaaaa cgacacgtaa gatcggcgta gtaacagccc
      361 tgttagtaag ttttgctttt acatcccagg cacagacggc caatcccggc aacggcaata
      421 ccggttcctc cgacaaagaa tggcgcatca gcgtaggccc tgaagcgggt ctgcccctcg
      481 gtaattattc caacggctat aactggtatt tcggcggaag cgcgcaattg gatattcccg
      541 ttatgcatag tctgttcgtc accgttaacg cggggtatga caatctcttc gccaaaagcg
      601 gtgagatccc cgggaccaac ctccaactga taccgttgaa ggcgggtctg aagtacttct
      661 tcataggtga cctcgtctat gtacaggcac aggccggcgc gaccatcgtt gcgaacaaaa
      721 cggatgtcct tgccgacaaa tcggcggcct tcacctatac tccccaggtg ggcgtattgc
      781 tgaagcttgc accgaagaac tatatcgatg tcggcttccg ttgggagcag accagctctt
      841 tttataatgg cgggtcgaac ctcagcaccc tggggcttcg cgtagcctat actttcgggc
      901 tttaatgatc cctcttttca ggcggcggcg cccctccggg gcgccgcttt tttttagccg
      961 ccgcttcccg gcaaagaccc ctctttgccg gacattgtcg aaaaagcggt ggctctattt
     1021 ttcgctgagg agccgccgct ttttttcatg aacccttaat ataggttaat ggcgggcata
     1081 tcctttgcct gtaattttgt gtcatcgttt aaacattaaa acttatattt tatggctaca
     1141 aaatgggttc tcgacccttc ccactcggaa gtggaattca aggtaaaaca tatgatgatc
     1201 tccaacgtca gcgggaaatt cacgaaattc gacgctgccg tcgagacgga aggcgaagac
     1261 ttcatgacgg caaaggtgac cttcacggcc gatgtcgatt ccatctccac gggcgccgaa
     1321 caacgggacg gacacctgaa atcccccgat ttcttcgatg ccgcacaatt cccgcagttg
     1381 aaattcgtag cctccaaata tgagaatgtg gataatgacg ggtcgtacga ggtgtggggt
     1441 gatctgacca tccatggagt gaccaaaaac gtaaaactgg atgcggaatt cggcggggtc
     1501 atcaaggatc cctggggaaa cacccgcgcg ggtatcaccg ccagcggcaa gatcaaccgg
     1561 aaggattttg gcctttcctg gcatggcgtg accgaggccg ggagcctgat cgtcagcgat
     1621 gaggtcagga tccatgtggg cctggaattc gtaaaagcat aatataagag aacaggctgt
     1681 ctgtttttgc ccttcaggcg gcctgttttc gttgtatatt tgcgcatgca ttatgtttcc
     1741 gtcgaagggt tgagcaagtc atacggggtc aaaccccttt tccagcagat aacgttccac
     1801 atcgaagaag gcgacaagat cgccctggtg gcccgcaacg gcagcgggaa atccaccctc
     1861 ctccgcatcc tttcaggcaa ggatacggcc gaaaacggca cggtctggat ccataaggat
     1921 gtcaccgtag ccctttttga acaggagccc gcctttatcg aagaccgctc catcctggac
     1981 aatatctttc accacgacca tcccgtcatc aacgccatca aggcctatga agcggccagc
     2041 gacgaagagg acgccgataa gatcaccgac gccatcgtcc ggctcgacga actgggcgcc
     2101 tgggatttcg acaccaaggt gaaacagatc ctggggaaat taaatatcca tcacctgcaa
     2161 cagccagcgg ggacactctc cggcggccag cgtaaacgcg tcgccctggc caagaccctc
     2221 atcgatatcg gcttcgagca caaacatgtc ctgctgatca tggacgagcc caccaaccac
     2281 ctcgacgtgg aaatggtgga gtggctggaa cattacctca accaggagaa agtgaccctc
     2341 ctgctggtga cccacgaccg ctactttctc gataacgtca ccgacgagat ctgggaaatg
     2401 gacggcagca gcctgcatgt ctataaaggc gactacgaga actacctcga gaaaaaagcc
     2461 gcccggctgg aaaatgatac ctccagcatc gaaaaagccc gcaacaccta tcgcaaggaa
     2521 ctggagtgga tgcgcaagca gccaaaggcc cgcaccacca agagcaagag ccgccaggat
     2581 aacttctatg tcgtcgaggc aaaggccaaa caacgcatcg aggaccagca ggtggaattg
     2641 cagatgaaga tgaaccgcct cggcggcaaa gtggccgaat tcaaaaaagt atacaagaac
     2701 tatggggaga aagtgatcct gaaaggtttc gactacacct tcaagcgcgg cgaacgggtg
     2761 ggtgtcatcg gccgcaacgg cgtcggcaaa tccaccttca tcaatatcct gcagggcctg
     2821 gaacccgcgg acagcggaaa ggtcaatatc ggcgagacgg tcattttcgg gaattacagc
     2881 cagcaggggc tcgtgataaa agaggatatg cgggtgatcg aattcgtaaa gaatatcgcc
     2941 gaacattttc cgctggcttc cggcggctcc ctgagcgcgg cacaattcct caatcttttc
     3001 ctcttcccgc ccgaacaaca atacacgtat atctccaaac tttccggcgg tgaaaagcgg
     3061 cggctccacc tgttgtcgat cctcttccgc aaccccaact tcctgatact cgatgaaccg
     3121 acaaacgatc tcgacctccc taccctggcc atcctcgaga acttcctgag cgaataccag
     3181 ggttgtttgc tgatcgtttc ccatgaccgg tatttcatgg acaggctggt agatcacctg
     3241 ctcgtgctgg aaggcgatgg cctgatccgg gacttcccgg gcaactactc ccaataccgg
     3301 gactggctaa aggacaacga agaagacgcg gcattcccaa
//
LOCUS       DATGFG010000567         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1119379, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000567 DATGFG010000000
VERSION     DATGFG010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119379"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VKQ52_10635"
     CDS             <1..1431
                     /locus_tag="VKQ52_10635"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLZ87691.1"
                     /translation="LRIAYKFIVPQYGTDRCGRLETKNGWIYEVAQWYPRMCVFDNVV
                     GWNTLPYLGQGEFYLEYGNIEYNVNVPSEMIVGGSGELLNPAEVLTPAQLKRYTEAKT
                     SEKTIALRGENEVTDPASRPQGKRLTWRFKCTNTRDVAWAASKAFIWDAARINLPSGK
                     KSLAESLYPVESAGDSAWGRSTEYVKGCIEYYSNYLYEFSYPAATNVAGNVGGMEYPG
                     IVFCGSRSRKGNLWGVTRHEFGHNWFPMIVGSNERKYAWMDEGFNTYINTVADSAFNK
                     GEYFRPVQTRARLAAPFFNPADPMMTIPDQMTSREWGILSYNKPGTGLELLREEILGK
                     DRFDYAFRYYVHQWAFKHPTPYDFFHAIENASGENLDWFWRGWVFNNWKIDQAVTAVD
                     YVQGDSTKGATITIENKGKLPMPATVEVKEENGNTSRVKLPVEIWMKSGIWKFHYPST
                     SKITQVTIDPDQRYPDSDPSNNTFHP"
     gene            1577..>2932
                     /gene="zwf"
                     /locus_tag="VKQ52_10640"
     CDS             1577..>2932
                     /gene="zwf"
                     /locus_tag="VKQ52_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLZ87692.1"
                     /translation="MTQKKVVPASITIFGAKGDLTWRKLIPALYNLYVDHHLPPIFHI
                     FCVDFLTVDDAGYRSWLLDGVNEFSRTGRADAQKWTSFADRITYVQGDFMKKETYDTL
                     KDNIQKFEDKEKIRGVRMFYFAVAPRFIEVIADTLCSRKLCNRDTLDRIVVEKPFGTD
                     LDSARKLNRYLGKRFKEKQIYHIDHYLGKEMVQNILAFRFANHVFEPLWNNEYIDHIQ
                     ISVAEEVSVGKRGGYYDSSGALRDMIQNHLLQLLCIIAMDCPKAYEAEAIRDAKIKVL
                     KHVRIFTEEQVFKNVIRGQYTQGIVHGQPRPAYREEEQVKPDSNTETFVAARFFIDSQ
                     RWKGVPFFLRTGKNMAKQSTTIMIQFKDSPHKIFKDDITPNRLIISIQPELEIGLLFE
                     GKVPGLHMKLLPMEMDFMYKDAYIESLPEAYEALLLDVLDGEATQFMRADQIEWAWKV
                     VM"
BASE COUNT          732 a          998 c          687 g          515 t
ORIGIN      
        1 ctcaggatcg cctataaatt catcgtcccc cagtacggta ccgaccgctg cggccgtctc
       61 gaaacaaaga atggctggat ctatgaagta gcccaatggt atccccgcat gtgcgtcttc
      121 gataatgtcg tcggctggaa cacgctgccc tatctcggcc agggcgaatt ctatctcgaa
      181 tacggcaaca tcgaatacaa cgtcaatgtg ccctccgaaa tgatcgtcgg cggcagcggc
      241 gaactcctca accccgccga ggtcctcacc ccggcccaac tgaaacgcta caccgaagca
      301 aagacaagcg agaagaccat cgccctccgg ggcgaaaacg aggtcaccga cccggccagc
      361 cgcccccagg gcaaacgcct cacctggcgc ttcaaatgca ccaacacccg cgacgtggcc
      421 tgggccgcct cgaaagcctt tatctgggat gcagcccgta ttaatctccc cagcggcaaa
      481 aaatccctcg ccgaatccct ctaccccgtg gaaagcgccg gcgactccgc ctggggccgc
      541 agcaccgaat atgtaaaggg ctgtatcgaa tactactcca actacctcta tgagttttcc
      601 tatcccgccg ccaccaacgt cgccggcaac gtcggcggga tggaataccc cggcatcgtc
      661 ttctgcggca gccgcagccg taaaggcaac ctctggggcg tcacccgcca cgaattcggc
      721 cacaactggt tcccgatgat cgtcggcagc aacgaacgca aatacgcctg gatggacgaa
      781 ggattcaaca cctatatcaa tacggtagcc gactcggcct tcaacaaagg cgaatacttc
      841 cgccccgtac agacccgcgc ccgcctcgca gcgcccttct tcaaccccgc cgaccccatg
      901 atgaccatcc ccgaccagat gacctcccgc gaatggggca tcctgtcgta taataaaccc
      961 ggcaccggcc tcgaactcct ccgcgaagag atcctcggca aagaccggtt cgactacgcc
     1021 ttccggtact atgtccacca gtgggccttc aaacatccca ccccctatga cttcttccac
     1081 gccatcgaga acgcctccgg cgaaaacctc gactggttct ggcgcggctg ggtcttcaac
     1141 aactggaaga tcgaccaggc cgtcaccgcc gtggactatg tccagggcga ctccacaaag
     1201 ggcgcaacca tcaccatcga gaacaaggga aagctgccca tgcccgccac cgtcgaggta
     1261 aaagaggaga acggcaatac cagccgcgtg aagctgcccg tggagatctg gatgaagagc
     1321 gggatttgga aattccacta tccctccacc agcaagatca cccaggtcac catcgacccc
     1381 gaccagcgat acccggacag cgatccgtcc aacaatacct tccatcctta acacctaatc
     1441 acaaaaaaag aggcccccac ggtgggcaaa accagggggc ctctttttcc aaatatgtcc
     1501 ggcaaatgaa atttaccggg gagccgcccc atcaggcggc tttcacttat atttgtacct
     1561 actcaagtcc aatcatatga cacaaaagaa agtagtcccc gcttcgatca ccatctttgg
     1621 ggcaaaaggc gacctgacct ggcgtaaact catccccgct ttgtacaatc tctacgtcga
     1681 ccaccacctt ccccccatct tccatatctt ctgtgtcgac ttcctcaccg tggacgacgc
     1741 aggctaccgc agctggctgc tcgatggggt aaacgaattc agccgcaccg gccgcgccga
     1801 cgcccagaaa tggacgtcct tcgccgaccg gatcacctat gtccagggtg acttcatgaa
     1861 gaaggagacc tacgacaccc tgaaagacaa tatccagaaa ttcgaggata aagaaaagat
     1921 aagaggcgtc cgcatgttct actttgccgt ggcgccccgt ttcatcgagg tcatcgccga
     1981 cacactctgc agccggaaac tctgcaaccg cgacaccctc gaccgtatcg tcgtcgaaaa
     2041 gccctttggc accgacctcg actcggcccg caaactcaac cgctatctcg gcaaacgctt
     2101 caaggaaaaa cagatctacc atatcgacca ctatctcggc aaggaaatgg tccagaatat
     2161 cctggccttc cgcttcgcca accatgtctt cgaaccgctg tggaacaacg agtacatcga
     2221 tcacatccag atcagcgtcg ccgaagaggt cagcgtcggc aaacgcggcg gctactacga
     2281 cagcagcggc gccctccgcg acatgatcca gaaccacctg ctgcaactgc tctgtatcat
     2341 cgccatggat tgcccgaaag cctacgaggc cgaagcgatc cgcgacgcca agataaaagt
     2401 gctcaagcat gtccgcatct tcacggaaga acaggtgttc aaaaacgtta tccgcgggca
     2461 gtatacccag ggtatcgtac atggccagcc ccgccccgcc taccgcgagg aagaacaggt
     2521 gaagcccgac tcgaacaccg agacctttgt cgccgcccgc ttctttatcg acagccagcg
     2581 ctggaaaggc gtccccttct tcctccgcac cggcaagaac atggcgaagc aatccacgac
     2641 catcatgatc cagttcaagg actcgcccca taagatcttc aaggacgata tcacccccaa
     2701 ccggctcatc atcagcatcc agcccgaact cgagatcgga ctcctcttcg agggcaaagt
     2761 tcccggcctc cacatgaaac tcctccccat ggaaatggac ttcatgtaca aggatgccta
     2821 catcgaatcg ctgcccgaag cctacgaggc cctgctcctc gacgtcctcg acggcgaagc
     2881 cacccaattc atgcgcgccg accagatcga atgggcctgg aaagtcgtca tg
//
LOCUS       DATGFG010000568         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1451366, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000568 DATGFG010000000
VERSION     DATGFG010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451366"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VKQ52_10645"
     CDS             complement(<1..987)
                     /locus_tag="VKQ52_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLZ87693.1"
                     /translation="MLKDSLLLVVSLLFIVSMLAMLSDKLRISYPIFLVIAGLGISLI
                     PGIPRIELDPDIVFVIFLPPLLYSAAWMTSWHEFWQLRRPIGLLGFGLVLCTAFTVAY
                     FSHWLIPNFSVSLGFLLGGIISPPDAVAATSVLQNLRVPKRVVSILEGESLVNDASSL
                     IVFRFAMAAILTGKFVFWRAGVDFLLVVFMGVVIGLVIAHIAYAIHKWLPTTSSIDTA
                     ITLICPYLMYIVAEHFHYSGVLAVVSGGLFLSYRSHELFSYDSRLQSQSVWNVLVFLL
                     NGVVFILIGLQLPAVVRGLGEYSLYAAIRYGIAISLLTIVVRILWVYPGAYLP"
     gene            1085..1576
                     /locus_tag="VKQ52_10650"
     CDS             1085..1576
                     /locus_tag="VKQ52_10650"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HLZ87694.1"
                     /translation="MKLIAAAFLALLSACQSPAGTPSAHKTDSVVPATTLVKAPPDST
                     TLGGEWFLQPVLPSDTAAGKTPTLQLDLGKSRFSGNTGCNSMHGQFWYGNTDSSLSFG
                     DKINMTRMACPGYNEPAFLKSLKNVSHYRLQNGVLTLLSDDRTELSRWMRKRATAPKA
                     LKA"
     gene            1677..1952
                     /locus_tag="VKQ52_10655"
     CDS             1677..1952
                     /locus_tag="VKQ52_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87695.1"
                     /translation="MAQTLVDLTRELLNSGVFNHLPDNEIARLHWMILQGQRDEELPL
                     QPLFTYWYRGDYYSSNASARLLQQCNEYLQRMSQPLIDVYGEEFYEA"
BASE COUNT          544 a          604 c          518 g          372 t
ORIGIN      
        1 gggcagatag gcgccgggat agacccaaag gatccggacg acgatggtca gcaggctgat
       61 ggcgatgcca taccggatgg ccgcgtacag ggaatattcc ccaagaccac gcacgacggc
      121 gggtagctgc aacccgatca ggatgaacac caccccgttg agcaggaata caaggacatt
      181 ccagacgctt tgtgactgca gacggctgtc atagctaaag agttcatgac tccggtagct
      241 gaggaaaagc ccaccgctca ccaccgccag cacgccggaa taatgaaagt gctccgcgac
      301 gatatacatc agatagggac agatcagggt aatcgcggta tcgatgctgg acgtagtggg
      361 cagccacttg tgaatggcat aggcgatatg cgcgatgacc agcccgatca cgacccccat
      421 aaaaaccacc agcaggaagt cgacacctgc ccgccagaac acaaatttcc ccgtgaggat
      481 cgccgccatg gcaaaccgga aaacgataag actcgatgca tcattcacca ggctctcgcc
      541 ctccaggatg ctgaccaccc gcttggggac ccgcaggttc tgcaacacac tggtggcggc
      601 taccgcatcc ggcggagaga tgatccctcc cagcaggaag ccaagcgata ccgaaaaatt
      661 ggggataagc caatgggaaa aataggccac cgtaaaggca gtacaaagca ccaatccaaa
      721 cccgagcagc ccgatcggtc ttcgcaactg ccagaactca tgccaggagg tcatccaggc
      781 cgcggaatag agtagcggcg gaagaaagat gacaaaaacg atatcgggat ccagctcgat
      841 ccgcgggatg ccggggataa gactgatccc cagcccagcg atcaccagga aaataggata
      901 ggagatcctc agcttatcgc tgagcatcgc cagcatcgac acgataaaaa gcagcgaaac
      961 gacgagtaaa aggctgtctt taagcataat gtaagataat atttatcgtg ctcccatcca
     1021 ccggggaaat ccttccccac gctgttggca cagtttttcc gcggatatcc caaaacacca
     1081 tcacatgaaa ctcattgctg ctgcttttct cgcccttttg agcgcctgcc agtcaccggc
     1141 agggacgccc tccgcacata agaccgactc cgtcgtacca gccaccaccc tggtaaaggc
     1201 gccgccggat agcaccaccc ttggcggtga atggttcctc caacccgtac tgccatcgga
     1261 taccgccgcc gggaaaacac cgaccctgca actggacctc ggtaaatccc gcttttcagg
     1321 caacaccggc tgcaattcga tgcacggaca attctggtat ggcaacaccg acagcagcct
     1381 ttcctttggc gacaagatca acatgacaag gatggcctgc ccgggataca acgagcccgc
     1441 attcctgaag agccttaaga acgtttccca ctaccggctg caaaacgggg tcctgaccct
     1501 tctctccgac gacagaacag agctgtcccg gtggatgcgg aaacgcgcaa ccgcaccaaa
     1561 ggccctgaaa gcctaatcca catcgaatcc acaatccgtt gccgacacag taggataaca
     1621 taagcttcat actgtggtaa catttttgct cgaactttcc attaaagaaa cctggtatgg
     1681 cacagacact ggttgatctg acgagggaat tgttgaacag cggggttttt aatcaccttc
     1741 cggacaacga gatcgcccgg ttgcactgga tgatcttaca gggacagcgg gacgaagagc
     1801 tgcccctgca accgttgttc acctattggt accggggaga ttactattcc tccaacgcct
     1861 cagctcgtct gttgcagcaa tgcaacgagt atttacaacg gatgagccag ccgctcatcg
     1921 atgtctacgg cgaggaattc tacgaagcct gagggctgta gtaaccgccg ggatcgctca
     1981 acaccttttt ccactgacgc tggtggctct cctgccattt cttatccggg tacacccc
//
LOCUS       DATGFG010000569         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1050021, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000569 DATGFG010000000
VERSION     DATGFG010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050021"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VKQ52_10660"
     CDS             <1..651
                     /locus_tag="VKQ52_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ87696.1"
                     /translation="PGRPDEVIGVVKNYHQRSFRQDYTPLILFMDPAYPWIPANYYSI
                     KVNTADIAGLVTGIKASWKNLFPESSFDYFFLDDFYDRQYQHERRFGQLFGWFSGLAI
                     IIACMGLFGLTAYTVARRTKEIGVRKVLGASVTGITRLLIWEFIRLIVIAGCLAMPLA
                     GWLIVEWMQGYAFRATLTWWQFALPLLLLIGITIGTTGWLTVRAARINPANALKEE"
     gene            complement(682..1050)
                     /locus_tag="VKQ52_10665"
     CDS             complement(682..1050)
                     /locus_tag="VKQ52_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ87697.1"
                     /translation="MTAIKESSTNQQNRQIVQDACPVTYTLGKIGGRWKPLIIYQLLD
                     GAKRYGYLKKAIPGITEKMLIQHLRELETDGLIVRVAKPVVPPHVEYSLSEDGMTLAP
                     ILGAMANWGLARRGAERTAD"
     gene            1129..2013
                     /locus_tag="VKQ52_10670"
     CDS             1129..2013
                     /locus_tag="VKQ52_10670"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HLZ87698.1"
                     /translation="MKYVLLGSLGNITKPLAQQLIAAGQEVTIVSSDAGKAPAIKTLG
                     ATPAIGSIEDVTFLTNTFRGADAAYIMIPPRFDANPWKQWIGGVGEKYAAAIKAAGLK
                     KIVFLSSIGADRPAGVGPVSGIHLAEVALNTLTGVDIRYLRPAYFYTNLLSSIGLIKQ
                     AGIYGNNYSADAGVVMVHPRDIAAVVAEELLGLTFKGSSVRYIAGDERNSREIAAVLG
                     AAIGKPDLPYVGFSDEDNHKGLLGAGLPEEVAQNYTEMGAAIRTGIMFADYKKHRPAL
                     SPTKLEDFAKEFAAAYNA"
     gene            complement(2097..2651)
                     /locus_tag="VKQ52_10675"
     CDS             complement(2097..2651)
                     /locus_tag="VKQ52_10675"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ87699.1"
                     /translation="MRIIQKAKRGDYPEYAEMYMKWIPDDGLLLQHLKDNFEENKKFI
                     YSLPESLLHYRYEEGKWSIKEILVHIVDDERIYAYRALCFARGEKEGLPGFDQDAYAA
                     YSGANERSPDSIFEEYEAVRNSTITLFKHLPEEAFDRMGHGTGTFAGATVRALGYHIA
                     GHEQHHMNVIRKYYLSGFAGGGNL"
     gene            2900..3187
                     /locus_tag="VKQ52_10680"
     CDS             2900..3187
                     /locus_tag="VKQ52_10680"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ87700.1"
                     /translation="MNQKLDTKRLERMCKALGDPYRIQMLEYIRNNPEWTQCTAITES
                     FNLAQSTVSHHLKQLVDVDLLHSEKEGRCTKYKINDEAFTEYVEYLKCFQQ"
     gene            3279..>4180
                     /locus_tag="VKQ52_10685"
     CDS             3279..>4180
                     /locus_tag="VKQ52_10685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ87701.1"
                     /translation="MRRIKEKNRGIATILSFALIPLTGLATDVYLPSLPAMAADLHVS
                     SGAIQNTLLVFLVSAGLSQLFVGSLLDSFGRYRISMAALLLFSLASFTIANTHSIYML
                     FAMRAVHGITVAIIVVAKRAYFLDSFEGERLKNYTSLFSIVWATAPIVAPFVGGYLQT
                     AFGWHGSFYFLGAFTLVILALDLIFGGESLQTFSPFKPAEIFQTYGSMLKTKDYGMAL
                     AIMGLSYSVLMVYGMTSPFIIEHLYHYSPIITGYSSLLSGVALMTGGIISKSIIHKPL
                     DRKIPVAMGLQAVIGIAMIAVSSL"
BASE COUNT          956 a         1261 c         1005 g          958 t
ORIGIN      
        1 cccggccgcc cggacgaggt catcggcgtc gtaaagaatt atcaccagcg atcgttccgg
       61 caggactaca cgccgcttat cctgttcatg gacccggcct atccctggat accggcaaac
      121 tattactcca taaaggtcaa caccgcagat atcgccggcc tcgtgaccgg cataaaagcc
      181 tcctggaaaa acctcttccc ggagtcttcc ttcgattatt tcttcctgga tgatttctac
      241 gatcgccaat accaacatga acgccgcttc ggtcaactgt tcggctggtt ctccggcctt
      301 gccatcatca tcgcctgtat gggattgttc ggtcttacgg cctataccgt cgcccggcgg
      361 acaaaggaga tcggcgtccg caaagtactg ggcgcatccg tgacggggat cacccgcctg
      421 ctgatctggg agttcatccg cctgatcgtt atcgcaggtt gcctggccat gccgctggcg
      481 ggatggctga tcgtcgaatg gatgcagggc tacgccttcc gcgccaccct cacctggtgg
      541 cagtttgcgc ttcccctgct gctgttgatc ggcatcacta tcggtacaac gggctggcta
      601 acggtaaggg ccgctcgcat caatccggca aacgccctga aggaagagta aattccaccc
      661 gcgcgctcaa ttccccttct ctcaatccgc cgttctctca gccccccttc tcgcgaggcc
      721 ccaattcgcc atcgcgccca ggataggggc gagcgtcatc ccgtcctccg aaagactgta
      781 ttcgacatgc ggcggaacca cgggttttgc cacccgtacg atcagcccgt ccgtctccag
      841 ttcccgcaga tgctggatca gcatcttctc ggtgatcccc gggatagcct tcttcagata
      901 gccataacgc tttgctccat cgagcaattg atagatgatc aatggcttcc acctgccgcc
      961 gatcttcccg agggtatagg tcacagggca ggcgtcctgg acgatctgcc ggttttgctg
     1021 gttggtagaa ctttctttta tcgcggtcat aatgatactt actttagggt gggtactccc
     1081 taaatcgaca gtaaagatat agctttgctt catcaaatca aaagagatat gaaatatgta
     1141 ctactggggt ccctgggaaa tatcaccaaa ccccttgcac agcaactgat agccgccgga
     1201 caggaagtaa ccatcgtaag cagcgacgcc ggcaaggccc cggccattaa gaccctcggc
     1261 gccacccccg ctatcggctc gatagaggac gtcacttttt taaccaatac cttccgcggc
     1321 gccgacgcgg cctatatcat gatcccgccc aggttcgatg caaatccctg gaagcaatgg
     1381 atcggtgggg taggggagaa atatgccgct gccataaaag ccgccgggct caagaagatc
     1441 gtctttctga gcagtatcgg ggcggataga ccagccgggg tgggacctgt ttcagggatc
     1501 cacctcgccg aagtggctct taacaccctc accggtgtcg atatccggta cctccgcccg
     1561 gcctatttct acactaactt attgagcagc atcgggttga tcaaacaggc tggcatatac
     1621 gggaataact atagcgcgga cgccggcgtc gtgatggtcc atccccgcga tatcgccgcc
     1681 gttgtcgcgg aggaattgct gggccttaca ttcaagggaa gcagtgtacg gtacatcgcc
     1741 ggcgacgaaa gaaacagcag agagatcgct gccgtgctcg gcgccgccat cggcaaaccc
     1801 gatctgccct atgtcggttt tagtgatgag gacaaccaca aaggcctgct tggcgccggt
     1861 cttcccgaag aggtcgccca aaactatacg gaaatgggcg ccgctattcg caccgggatc
     1921 atgttcgccg actataaaaa gcaccggccg gccctttcgc cgaccaaact ggaggatttc
     1981 gcaaaagaat tcgccgccgc ctacaacgct taaaaatccg gccggctcaa acgagccggc
     2041 tacttttttt aacggaatcg gtcgttttta ttgaactttg gggaacccgg catcatttac
     2101 aaattgccgc ccccggcgaa accggacaaa taatactttc ggataacatt catatgatgc
     2161 tgttcatgcc ctgcgatatg ataccccaat gcccttaccg ttgcaccggc aaaagtcccc
     2221 gtcccatgtc ccatccggtc aaacgcctct tcgggcaggt gcttaaatag cgtgatggtt
     2281 gaattcctga cggcctcata ctcttcgaaa atactgtccg gcgaccgttc atttgccccg
     2341 gaataagcag cataggcgtc ctggtcgaat ccggggagac cttccttctc accacgggcg
     2401 aaacaaagcg ccctgtacgc atagatcctt tcgtcatcga cgatgtgaac caatatttct
     2461 ttgatactcc attttccctc ctcgtatcgg tagtgtaaca gggattcggg aagagaataa
     2521 atgaattttt tattttcctc gaaattgtcc ttcaggtgtt gcagcagcag gccatcatcc
     2581 gggatccatt tcatatacat ctctgcatat tcagggtagt cgcccctttt cgctttttgt
     2641 attattctca ttgtcgtatt ggttgagtag ggtttccagc aggtttacgg cgccggttat
     2701 ttcttcctcg tccagtgggg caaaaccgat tcggatgttg cttcccttac aaaattaggg
     2761 gaagcgatca tatatcctac gcgaagaggg gccgggaagg agcagtttgc ccgcacccgc
     2821 cctggcccgt tgagcacgcg caggtcctct gtcgccccgc cgacattgtt atatcgaaaa
     2881 acatcgatac atttgtgtcg tgaatcagaa actcgatacg aaacgactcg aaaggatgtg
     2941 taaggcgctc ggggatccct accgcatcca aatgctcgag tacatccgga ataacccgga
     3001 atggacccaa tgtaccgcca tcacggagag cttcaacctc gcccaatcta ccgtatccca
     3061 ccacctgaaa caactcgtgg acgtcgacct cctccatagc gaaaaagaag gccgatgcac
     3121 aaaatacaag atcaacgacg aggcattcac ggagtacgtc gaatacctaa aatgcttcca
     3181 gcaataggcg aagcccgggg gccccttttt tttcgaacaa tcgatcgata tatttcgttt
     3241 tatcgaataa acacaaacaa acaagaaaac caacgatcat gagacgcata aaagaaaaga
     3301 accgcgggat agcaacgatc ttatcctttg cccttatccc gctcaccgga ctggcaaccg
     3361 atgtctacct gccctcctta ccagccatgg cggccgacct gcacgtaagc agcggcgcca
     3421 tccagaatac cttactcgtc ttcctcgtaa gcgccggcct cagccaactc ttcgtcggca
     3481 gcctcctcga cagcttcggt cgctatcgga tcagtatggc cgccctgctg ctcttctccc
     3541 tggcaagctt caccatcgcc aacacccata gcatctatat gctgttcgcc atgcgcgcgg
     3601 tacacggtat aacagtggcg ataatagtag tggcaaaacg cgcctacttc ctcgatagtt
     3661 tcgaaggcga acgcctcaag aactacacca gcctgttctc catcgtctgg gccactgcgc
     3721 ccatcgtcgc gcccttcgtc ggtggttatc tccaaacagc cttcggctgg cacggtagct
     3781 tctacttcct cggcgccttc accctcgtta tcctggccct cgatctcatc ttcggcggtg
     3841 aatccctgca gacatttagt ccctttaaac cggccgagat cttccaaacc tacggctcca
     3901 tgttaaagac aaaggactat ggaatggccc tcgcgatcat gggtctcagc tacagcgtcc
     3961 tcatggtata cgggatgaca agccccttta tcatcgaaca tctctatcac tattcaccca
     4021 tcatcacggg ctatagctcc ctgctttcgg gagtagccct gatgaccggt ggcatcatct
     4081 cgaaatccat catccataaa ccactcgaca ggaagatccc cgtagccatg ggcctccagg
     4141 cggtgatcgg catcgccatg atcgcggtat ccagcctctg
//
LOCUS       DATGFG010000570         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1020823, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000570 DATGFG010000000
VERSION     DATGFG010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020823"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VKQ52_10690"
     CDS             <1..198
                     /locus_tag="VKQ52_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLZ87702.1"
                     /translation="FHGESDRRTGFVQGEMLYRSLKVLGRSVEYVRHPGATHELTRSG
                     DNRQRIDQMLRTYEFFQRYVQ"
     gene            476..1753
                     /locus_tag="VKQ52_10695"
     CDS             476..1753
                     /locus_tag="VKQ52_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87703.1"
                     /translation="MNRLCTLVTLTIILGLLHTSATAQQIAGIASVSRHELDTETDLL
                     GVNETPVLKAQPWERTPDRITGHIDHSKLNNMKSRTEALVHLFQDSCFSAGDYTPVWH
                     GEYFSGRSLTGSLLKFAAQCSFYGGEKNTDKKADLLILANDLTPLLGHLTVYNNAYLT
                     ITATITRRNGCFYFELPQAEAGEQATSSNGAPAGLTTSFWLVTTDSTRLPYMPVTRKE
                     YLLEARKQLISDTDRISTEWRNKITIRPTTAQEAEKQQELEQLKALYTGTELEARTRT
                     YLAGFLTDEAYLQANIRKATALQRATLHFVDSLLHTDAAELNRPAVIAPSDNTQTSGE
                     QAAQPVDGNTPNADAGVDKAFAFHGFADGQPGATLLVKQNPTFFDPYLSEDKPQFFLV
                     GWRYDSSDPLAADLNRQLHQNFNGQGVQEMLGK"
     gene            2120..2722
                     /locus_tag="VKQ52_10700"
     CDS             2120..2722
                     /locus_tag="VKQ52_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ87704.1"
                     /translation="MKAEVKYTDVELIEAISAGTDLNKAIQFIYRQHAEATSSFIRHY
                     GGSEQDADDIFQEAVVAFIDVVRKGKFRMESNVRTFLASIAKNCWFNELKKRERADHR
                     NRQFELGRDQNEPDVAEHINDLERKKQLRDLVDGLGESCRKILLLFYYENMTMKEIVY
                     HLPYENEQVVRNKKYKCLQQLTGLIKDNPAVARQMQEIIK"
     gene            2731..3534
                     /locus_tag="VKQ52_10705"
     CDS             2731..3534
                     /locus_tag="VKQ52_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ87705.1"
                     /translation="MTPDQRHLLIDHLDESLRGRATATLEEIIGNDPEAEKEWNYLRL
                     AVDAVRDAGLNQQVAAARAAWKAEFEAADSMAPVKTMPSPQSGSTGKVRTLYRYTLRA
                     AACILIITGSSALYKYVSVSSGSLYDRYYTTYTLGATRGAGDTDPIVQAYDAKDWTTV
                     RNLANTAKPRTNQTDFLAGMACLEQKQYDEAISHFEQIIAVNTHAGTSYYQDEAEYYL
                     AISWLAHKNVNEAMPILEKILTDQHHQYHEKVMKMSFFDLRLAQYKENK"
BASE COUNT          936 a         1260 c          735 g          611 t
ORIGIN      
        1 ttccacggcg agagcgatcg ccgcaccggc ttcgtccagg gcgaaatgct ctaccgcagc
       61 cttaaagtcc ttggccgatc ggtggaatat gtccgccatc ccggagccac tcatgagctc
      121 acccgcagcg gcgacaaccg ccagcgcatc gaccagatgc tgagaaccta tgaattcttc
      181 cagcggtatg tacagtaaca agcggtagat acccttctca ccatcctgac catcgctcat
      241 ggccggcaag ggccggcccg gccccatccc atcccgggac aaaagacaag aattctttcc
      301 aatcgcggga cgccgcgcgg cgccccgcaa acggcatcct cctccaaacc aattccttgc
      361 atttttggct cgtcgattgc cctcatgggg catctcacct cccatatccc ccgaaagatc
      421 ttgcacgaca acaaccacta tcccatggta ccccaaaccc taaagaaagc aagacatgaa
      481 tcgtctctgt accctggtaa ccctgactat catcctcggc ctgttgcata cctccgcgac
      541 cgctcagcaa attgccggca tcgcctctgt ctcccggcat gaattagata ccgaaaccga
      601 tctgcttggc gttaacgaaa cgcccgtcct caaagctcaa ccctgggaaa gaacccccga
      661 tcgcattaca ggtcacatcg accatagcaa actcaacaat atgaaaagca ggaccgaagc
      721 cctggtccat ctcttccagg actcctgctt ctccgccggc gactacaccc ccgtatggca
      781 tggcgaatac ttctccggcc ggtcactcac cggctccctg ctgaaattcg ccgcccagtg
      841 cagcttctat ggtggagaaa agaacaccga caaaaaagcc gaccttctca tcctcgcaaa
      901 cgacctcacc cccctcctcg gccaccttac ggtctacaac aatgcctatc tcaccatcac
      961 ggctaccatc actcgcagga acggctgctt ttacttcgag ctcccccagg ccgaagcagg
     1021 cgaacaagcc acttcttcca acggcgcccc cgccggcctc accacttcct tctggctcgt
     1081 cacaaccgac agcacccgcc tcccctacat gcctgtcacc cggaaagagt acctcctcga
     1141 agcccgcaaa cagctcatca gcgacaccga tcgcatctcc accgaatggc ggaacaagat
     1201 caccatccgc cccaccaccg cccaggaggc cgaaaaacag caggaactcg aacaactaaa
     1261 agccctctac accggcacag aactcgaagc ccgcacccgc acctatctcg ccggcttcct
     1321 caccgacgag gcctacctcc aggcaaacat caggaaggcc actgctctcc agcgcgctac
     1381 ccttcatttc gtcgacagcc tcctgcacac cgacgctgcc gaactcaacc gccccgcggt
     1441 catcgccccc tccgacaaca cacagacctc cggcgaacaa gccgcccaac ccgtcgacgg
     1501 caacactccc aacgccgacg caggcgtcga caaggccttc gccttccacg gcttcgccga
     1561 cggccaaccc ggcgccaccc tgcttgttaa acaaaacccc accttcttcg acccctatct
     1621 cagcgaagac aaaccccagt tcttcctcgt cggctggcgc tacgacagct cggatcccct
     1681 cgccgccgac ctcaaccggc aactccatca aaacttcaac ggacaagggg tacaggaaat
     1741 gctcggcaaa taactatcag ccggcaccgc tctccccagc accccacact ccatttacgg
     1801 cccccaccca ccccccaacc ccaccaggtg ggggccgtcc tatccccctc aacctaccca
     1861 aacaccccag gcgctcacgc aaaatcaccc caatcacccc agtcagatca ataaatttta
     1921 atccgggccc aaacctcctg actaccaact ccctacactc ctcccaccgc tccgggcacg
     1981 catttccggc aaccgcctcc cacctccccg gggccaatct aacttcatga catacaattg
     2041 catgccattc tttcgttact atagtacaca aaaaactacc ctgagcggtt acgattcccc
     2101 aaatagaatt gactaaataa tgaaagccga ggtaaaatat actgatgtcg aattgatcga
     2161 agccatcagt gccggtaccg atctgaacaa agcaattcag ttcatctacc ggcaacatgc
     2221 cgaagcaaca agctctttta ttcgtcacta tggcggcagc gaacaggacg cagacgatat
     2281 cttccaggaa gccgtcgtcg ccttcatcga tgtcgtacgc aaaggaaagt tccggatgga
     2341 atccaacgtt cgcaccttcc tcgcttccat agcaaagaat tgctggttca acgagctgaa
     2401 aaaacgggaa cgcgcagatc accgtaaccg tcagttcgag ctgggccgcg atcaaaacga
     2461 acccgatgtc gccgagcata taaacgatct ggaaaggaaa aaacagctcc gcgacctggt
     2521 agatggcctg ggagaatcct gtcgaaagat cctcctgctc ttctactacg agaacatgac
     2581 gatgaaagag attgtgtatc acctgccgta tgagaacgaa caggtggtaa gaaacaaaaa
     2641 gtacaagtgc cttcagcaac tgacagggct tattaaagat aatcctgccg ttgcacggca
     2701 aatgcaggaa attatcaaat agcctatact atgacacccg accaacgaca tttactgatc
     2761 gatcatctcg atgaaagcct ccggggcagg gccacagcca ccctcgaaga gatcatcggg
     2821 aacgatcccg aagccgaaaa ggaatggaac tacctgcgtc tcgccgtaga cgcggtaaga
     2881 gatgccgggt tgaaccaaca ggtcgccgcc gcccgcgccg cctggaaagc cgaattcgaa
     2941 gccgccgaca gcatggctcc cgtaaaaacc atgccttcgc cccagtccgg ctccaccggc
     3001 aaggtccgca ccctctatcg ctataccctc cgcgccgccg cctgtatcct catcataacc
     3061 ggcagctccg ccctctataa atacgtctcc gtaagctccg gctcactgta cgaccgctat
     3121 tatactacct ataccctcgg cgccacccgc ggcgccggcg acaccgaccc gatcgtccag
     3181 gcatacgacg caaaagactg gaccaccgtc cgcaacctcg ccaacacggc aaaaccgcgg
     3241 accaaccaaa ccgacttcct cgccggcatg gcctgtctcg aacaaaaaca atacgacgag
     3301 gcgatcagcc acttcgaaca gatcatcgcc gtaaatacac acgccggaac cagctactac
     3361 caggacgaag ccgaatacta tttggccatc agctggctgg cgcacaagaa cgtcaacgaa
     3421 gccatgccca tcctggaaaa gatcctgacc gaccagcacc accaatacca cgaaaaagtc
     3481 atgaaaatgt cctttttcga tctccggctg gcccaataca aggaaaataa ataagaaaac
     3541 cg
//
LOCUS       DATGFG010000571         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_122738, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000571 DATGFG010000000
VERSION     DATGFG010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122738"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VKQ52_10710"
     CDS             <1..339
                     /locus_tag="VKQ52_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87706.1"
                     /translation="SILCKKSVLQLKAYEDKALGEDTATIEYLARMDSLHFLNEMPEL
                     YIYVYHGNNSWNYDHWGAIFRCSTALLPEESKIVADILSRKYSVPEGSQLLDTIMERV
                     HSEEVLISRS"
     gene            336..2381
                     /locus_tag="VKQ52_10715"
     CDS             336..2381
                     /locus_tag="VKQ52_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylase"
                     /protein_id="HLZ87707.1"
                     /translation="MRKLFIFLLSLLHLSGHAGPPGGEIVWDRYGVPHIYGETAMGMY
                     YAFGWAQMHNHANLILKLYGQARGKAAEYWGENYLPSDKQVLLFGLPDLAAREYAQQD
                     PVFKGMLDAFVAGLNAYARVHRDQIDSASARVLPVTVQDVFAHGLRVVFLRFVAGEDL
                     GMASRKLQAGSNALAIAPSRSAQKTALLLANPHLPWGDLFTWFEAHLQSPGFNAYGAA
                     VVGLPVLNIAFNDHLGWTHTVNTIDVCDRYELSVRGDGYLLDGVVRPFEKRAVVLKSR
                     QPDGRLKEQTVELVHSRHGPVIGIKGDKAYAIRIAGLENPRIFYQWHLMAAATDWSQF
                     ERALKMMQLPMFNVIYADGVGNIFYLFDGNVPDRAEGDWQFWHGTVDGTNSRDIWNKT
                     LPYADLPKLLNPRTGFIQNANDPPWTCTYPPVLAPDRYPAYLSPRGMGLRPQRAVNMI
                     RGDSLITLPALAAYKLNTGMEAADRFLTDLLAAAGEYGTDTLVKKAVAVLRGWDRATH
                     ADSRGAVLFARWFDKLSPEMFSRQWAPDDPVETPVGLKDRKGAVGLLAQAAGEVIRDY
                     DSLDVAWGDVYRFRVGGLDYPANGGPEQYGIYRTIYFTRDKDNKYRAVAGDSYVAVTE
                     FGKKVKAWVLLSYGNASQPGSRHLGDQLSLLARGELRPALLGKEEIEKNAEETERLK"
     gene            2448..2930
                     /locus_tag="VKQ52_10720"
     CDS             2448..2930
                     /locus_tag="VKQ52_10720"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HLZ87708.1"
                     /translation="MKKEFRLLYEEYASAQELPAPDAFLLSRALAATRDAYAPYSRFR
                     VGAAARLANGEVITGTNQENASYPVGICAERTLLSVAGALYPGVAIETLAVSYYNEHG
                     VSERPIAPCGICRQSLQEFEQRTGRAMRVILGGASGKVIVLEGSGLLLPFAFSGDELK
                     "
     gene            3089..>4005
                     /locus_tag="VKQ52_10725"
     CDS             3089..>4005
                     /locus_tag="VKQ52_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ87709.1"
                     /translation="MARITPLTFQAGVPCVLLLLSFSLFFVSSCKEHADAAGSNHKPK
                     DGDPALVDVLVAYARPISNEIEANGSVVANEYVELHPEISGRIVYLNVPEGAHVAKGT
                     VIARINDADLQATLAKSKAQLDLYQKTEERERKLLDINGINQADYDLAVSNVSSTRAD
                     IDYTQAQLDKTIIRAPFDGVVGLRQVSPGAYVTPTNIIATVQQLDKTKVDFTIPEQYS
                     SVVRKGSLVDVQIDATANVRRKGLIVATEPQINLSSRNLKVRALLQDGVAHPGSFVKV
                     FVNAGADKRAIMVPTNCIIPEDRNNQVILV"
BASE COUNT          832 a          997 c         1274 g          902 t
ORIGIN      
        1 agtatccttt gcaagaagag tgttttgcag ctcaaagcat acgaggacaa ggcgcttggt
       61 gaggataccg cgacgataga gtatctcgcc cgtatggaca gtctgcattt tctgaatgaa
      121 atgccggagc tttatatcta tgtatatcat ggcaacaatt cgtggaatta tgatcattgg
      181 ggcgctatct tccggtgcag cacggctttg ctgcccgagg aatcaaagat cgtcgccgat
      241 atcctgagcc ggaaatactc cgttcccgaa gggtcgcagt tactcgatac gatcatggag
      301 cgcgtgcatt cggaagaagt cctaataagc cggtcatgag aaaactattc attttccttt
      361 tgagtctttt gcatctttcc ggtcacgcgg ggccacccgg cggggagatc gtctgggaca
      421 ggtatggggt tccgcatatt tatggagaga cggcgatggg catgtattac gcctttggct
      481 gggcacagat gcacaaccat gccaacctga tcctgaagtt atacggacag gcgaggggaa
      541 aagcggcgga atactggggt gaaaattatc tgccatccga taagcaggtg ttgttgttcg
      601 gtttaccgga tctggcggct cgggagtatg cgcaacagga cccggtgttc aaagggatgc
      661 tggacgcttt tgtggccggg ttgaatgcct atgcccgggt tcaccgggat cagatcgatt
      721 cggcgagtgc gcgggtgctt cctgttacgg tgcaggacgt ctttgcccat ggtcttcggg
      781 ttgtctttct ccgtttcgtt gcgggagagg atctggggat ggcgagccgc aaattgcagg
      841 ccgggtcgaa tgccctggcc attgcaccgt cgcgatcggc gcaaaagacc gccttgttgc
      901 tggccaatcc gcatttgccc tggggggatc tgttcacctg gttcgaagcg catctgcaat
      961 cgcccggttt caatgcttat ggggccgccg ttgtcgggct tcctgtgctg aacatcgcct
     1021 ttaacgatca cctgggctgg acgcatactg tcaataccat cgatgtctgc gaccggtatg
     1081 aattgagcgt acggggagat ggttatttat tggacggggt agtgcggcca ttcgaaaagc
     1141 gagccgttgt cctgaagagc cggcagccgg atgggaggct gaaggagcag acggtggagt
     1201 tggttcattc ccgtcacggt cccgtcatcg ggataaaggg agataaggcg tatgccatcc
     1261 ggatcgccgg gctggagaac ccccggatct tttatcaatg gcatcttatg gcggcggcta
     1321 ccgactggag ccagttcgaa cgtgcgctaa agatgatgca gctgccgatg ttcaatgtca
     1381 tctatgcgga tggcgtgggg aatatcttct acttatttga cgggaatgtt cccgaccggg
     1441 cggagggcga ctggcagttc tggcatggga ccgtggacgg cacgaattcc agggatatct
     1501 ggaacaagac gcttccgtat gccgacctgc cgaagctgct gaatccccgg acggggttta
     1561 ttcagaatgc gaatgatccg ccctggacct gtacgtatcc gccggtgctg gcgcccgacc
     1621 ggtatccggc ttatctgtct ccgcggggga tgggtttgcg tccgcagcgg gccgtcaata
     1681 tgatcagggg tgattcgctg atcacgctcc cggcgctggc cgcgtacaaa ttgaatacgg
     1741 gaatggaggc tgcggatcgt tttcttaccg acctgctggc ggcagccggc gagtacggca
     1801 ccgataccct ggtaaagaag gcggtggccg tgttacgcgg gtgggatcgg gcaacgcatg
     1861 cggacagccg gggggcggtt ctgtttgccc ggtggttcga caagctctcc ccggagatgt
     1921 tcagccggca gtgggcgcct gatgatcctg tagagacccc ggtggggctc aaagacagaa
     1981 aaggggcggt tgggctgctg gcgcaggcgg cgggtgaggt tatccgggat tatgacagcc
     2041 tggatgtggc atggggggat gtataccggt tccgggtagg tggattggat tatcctgcca
     2101 atggagggcc ggagcagtat ggcatctacc ggaccattta ttttaccagg gacaaggaca
     2161 ataaataccg ggccgttgcc ggcgacagct atgtggccgt tacggagttc gggaaaaaag
     2221 tgaaggcctg ggtattgttg agttacggca atgcttcgca gccggggagc aggcatttgg
     2281 gggatcagtt atcgctgctg gcccggggtg aactgcggcc ggccctgctg gggaaggagg
     2341 agatcgaaaa gaatgcggag gagaccgaac ggctcaaata gagttatccc tgcgggccat
     2401 aaatagagta tttttgcggg ccatcccatc aacagggggc tcgcgacatg aaaaaagaat
     2461 tccgtctttt gtacgaagaa tatgcgtcgg cgcaggaact gcctgctccc gacgcatttt
     2521 tgctttcaag ggcccttgcc gccaccaggg atgcctatgc gccttattcc cggttcaggg
     2581 ttggagccgc cgccaggctg gccaacgggg aggtgatcac cggcaccaac caggagaatg
     2641 cctcttaccc ggtgggtatt tgcgctgagc ggacgctttt gtcggtggcg ggtgccttgt
     2701 atcccggtgt ggcgatcgag acccttgcgg tgagctatta caatgagcac ggggtcagcg
     2761 agcggccgat cgctccctgc ggcatctgcc ggcagtcgtt gcaggaattc gagcagcgga
     2821 cgggcagggc gatgcgggtt atcctgggtg gagcgagcgg gaaggtgatc gttctcgagg
     2881 ggtcggggtt gctgttgccc ttcgcttttt cgggggacga gctgaaatga gaggtttttt
     2941 acgaagatcg cagccattta ccgaccataa gtccatttgg gggttcatcc aataaacgag
     3001 ccggttcgtc caggagaggt caaatctttc tatctttgcg gaccttcagg ggaagccatt
     3061 cccgcctgga tacaaaaaat aaccgttaat ggctcgtatc actccgctaa cctttcaggc
     3121 aggggttccc tgtgtgctgt tgctattatc ctttagcctt tttttcgtgt cttcctgtaa
     3181 ggaacatgcc gatgccgccg gttccaacca caaaccgaag gacggtgatc cggcccttgt
     3241 ggatgtgctt gtcgcgtatg cccgtcctat cagcaatgag atagaggcga atggcagtgt
     3301 cgtcgccaac gagtatgtgg aattgcatcc cgagatcagc gggcggatcg tctacctgaa
     3361 tgtccccgag ggggcccacg tggcgaaggg taccgttatc gcccggatca acgatgccga
     3421 tctgcaggcc acgctggcga aatcgaaggc ccagctggac ctttatcaaa aaacggagga
     3481 gagggaacgc aaactgctgg atatcaacgg cataaatcag gcagactacg atcttgccgt
     3541 aagcaatgtc agcagcacca gggcggatat cgattatacg caggcccagt tggacaagac
     3601 cattatccgg gccccttttg acggggtggt cgggttacga caggtgagtc ccggggccta
     3661 tgtcactcct accaatatta tagccaccgt ccagcaactg gacaagacca aggtggattt
     3721 caccattccc gagcaatata gttctgtcgt gcggaagggc tccctggtgg atgtccagat
     3781 agatgcgacg gccaatgtcc ggcgcaaggg gcttatcgtg gccacggagc cccagatcaa
     3841 tctctccagc cgcaacctga aggtacgggc cctgttgcag gacggggtag cccatccggg
     3901 ttcctttgtc aaggtatttg tcaatgcggg tgcggacaaa cgggcgatca tggttcctac
     3961 caactgtatc attcccgagg acaggaacaa ccaggtgatc ctggt
//
LOCUS       DATGFG010000572         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_389130, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000572 DATGFG010000000
VERSION     DATGFG010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389130"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VKQ52_10730"
     CDS             complement(<1..919)
                     /locus_tag="VKQ52_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ87710.1"
                     /translation="MRIPLLPTLCLFAASVFFLPARGQSLSQLLKKLSTATTDSERAD
                     LYYSLSTRYWTGNTDSLLLLADLSLQAAKRDGYEKGVALALLSKGIGFGAKQEYSEAL
                     NCYLQALRISEQLKMDGLRSNLYDDMGNVYSHLKDYSRATEYHQDALKIAQRNGDRHE
                     TAVLLANIAENYKNMGAYDSALAYNVRALPIMESLHDSVVIAIILLNTGDDYNKKGQP
                     EKALPYFQRCDAMAQRIGDAEDIAWSAVSVADSYRRQRRYALSIRQARAALDSARRLD
                     LGELIEECYSVLYSAYRENGDFRQALDYRN"
     gene            complement(1479..>2841)
                     /locus_tag="VKQ52_10735"
     CDS             complement(1479..>2841)
                     /locus_tag="VKQ52_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ87711.1"
                     /translation="FRSTSTASEIFFYTAADGFAGLAIVRYSTTNGEQSACYIRFPMD
                     ARTRNLSGHEKRKLRIFKKMNLAFGLTPGILFPTAAWLLGIRLSAPAWFFAAAGPLLV
                     CILSAILIRLGQFERARLFFFILHPLLLTGVYLLRIDPGTDLFFICYGVLSVFFLQNL
                     YHVIFSFSLSLTCYFMAHGHGRAALVSLETSNIDLYLFNRLLAIFFIFYGIFLIRNEN
                     DRYQLQLNELNSLKSKLFSVIAHDLRGPLHAMVHLFKHMVQYDLPADEIKELIPEVAR
                     GLGETTGQIENLLEWAKSQMQSEVIRPQLLEISQVIHDVLSFLHLQVEAKELRIEKRL
                     SQPVYVYADKEMMRLVLRNLLSNAIKFTPERGVITVGASESPTDIEVYVRDTGTGISP
                     ENLERLAQNVQYSTPGTANESGTGLGLLLCREFLSRNGGYLEVASQLGKGSTFSFSLP
                     KQI"
BASE COUNT          725 a          829 c          683 g          604 t
ORIGIN      
        1 aattgcggta atccagggcc tgccggaaat ctccgttctc ccggtaagcg gaataaagga
       61 cggaataaca ttcttcgata agctcgccga gatcgagccg ccgcgcactg tccaacgcgg
      121 ccctcgcctg ccggatactc agcgcatacc ggcgctgcct gcggtacgaa tcagccaccg
      181 acaccgccga ccaggcaata tcctccgcgt cgccgatccg ctgtgccatc gcgtcacacc
      241 gctgaaaata ggggagggcc ttctccggtt gccccttctt gttatagtca tcacccgtat
      301 tcaacaggat aatggcaatc accaccgagt catgcagcga ttccatgatc ggcaacgcac
      361 gaacattata cgccagcgcc gagtcataag cccccatatt tttatagttc tccgcgatat
      421 tggcgagcag caccgccgtc tcatgccggt caccattcct ctgcgctatc ttcaaggcgt
      481 cctggtgata ttcagtggcc cgggaataat ccttcaggtg ggaataaaca tttcccatat
      541 catcgtacag gttcgaccgg agcccgtcca tcttcaactg ctcgctgatc cgcaatgcct
      601 gcaggtaaca gttcagcgcc tccgagtact cctgcttcgc gccaaaaccg atacccttcg
      661 acaaaagcgc aagcgctacc cccttttcat aaccatcccg cttcgccgcc tgcagggaga
      721 gatcggccag caacaaaaga ctgtcggtat tcccggtcca gtaccgggtg gaaagagaat
      781 aatagaggtc tgccctctcc gaatcggtag tggcggtgga caatttcttt agtaactggg
      841 atagggactg accccgggca ggcaaaaaaa agacagacgc agcgaacagg cacaaggtcg
      901 gcaacagggg tattctcatc cggatactgg tatttcccta caagtaaatg aaaatatctt
      961 acaggtactg tagtaatatt tctcattttg acgcttattt gcgcccaggc aaaatagaag
     1021 cattactaca atgaatgggc aaaaggcgtt ttatctttat tgtagaaggc ccgtttttaa
     1081 ggtccaattc ctgtgagcct gattcgtccc tgaggcacaa caccctgaac ttgcatactt
     1141 cggtacccga ttgctcttat aaagctgcat ccaccccggt gcagctttct tcatttcgcc
     1201 ccgcccgcaa aaaagccgct cgtaaatgaa ccggtcctaa aaataaaaaa aggggtttgt
     1261 ggaaacaaac cccgctaacc tatccccctt gagcggggac tactaaactc aagctgtgct
     1321 attctgcggt aaaaatacag cgcagccgat cgtatccttg tagtaaacta actaaaatga
     1381 gaaccggcat gcatacatat cgtccgaagc ggcattcata tcaagggaac tggcgttcat
     1441 atcatggcaa ctggcgttca tttcacggaa accggcgttc atatctgctt gggaagcgaa
     1501 aaagagaaag tgctgccctt acccagctga ctcgccactt ccaggtagcc gccattcctg
     1561 ctcaggaact ccctgcacaa caacaacccc agccccgttc cgctctcatt cgccgtgcca
     1621 ggtgtcgaat actggacatt ctgcgccagc ctctccaggt tctcgggact gatgccggtg
     1681 ccggtatccc gcacatacac ttcgatatcg gtcggactct cgctcgcccc gactgtaatg
     1741 acccccctct ccggagtgaa cttgatcgca ttggacaaca ggttgcgcag gacgagacgc
     1801 atcatctcct tatccgcata cacatacacc ggctggctga gccttttctc gatccgcagt
     1861 tccttcgcct ccacctgcaa atgcaggaag ctcaggacgt cgtgtatcac ctgcgagatc
     1921 tccagcaact gcggccggat aacctccgac tgcatctggc tcttcgccca ttccagcagg
     1981 ttctcgatct gccccgtggt ctctccaagc cccctggcca cctccgggat cagctccttg
     2041 atctcatcgg ccggcagatc atattgcacc atatgcttga aaagatgcac catcgcatgc
     2101 aacggccccc gcaggtcgtg cgctatcacc gagaacaact tgctcttcag agaattgagc
     2161 tcgttcagct gcaattggta acggtcgttc tcattccgga tcaggaaaat gccgtagaag
     2221 ataaagaaga tggcgaggag ccggttgaac aggtacagat cgatattgct cgtttccagg
     2281 ctcacaagcg cagccctgcc atgcccgtgc gccatgaaat agcaagtaag cgaaagcgaa
     2341 aaggaaaaaa tgacatgata gaggttttgc aggaagaaca ccgataacac cccataacag
     2401 ataaagaaca ggtcggtacc gggatctatc cgcaacagat aaaccccggt gagcaacagg
     2461 ggatgcagga taaagaagaa aagccgggcc cgctcgaact gcccgagccg gatgaggatg
     2521 gcagagagga tacagaccag tagcggaccg gcagcagcga aaaaccatgc cggcgcggaa
     2581 agccggatcc ccagcagcca ggcggccgtt gggaacaata tgccaggggt aagaccaaag
     2641 gcaaggttca tcttcttgaa aatcctcagc ttccgcttct catgtccgct aagattcctt
     2701 gttcgcgcat ccatcgggaa gcgaatgtaa caggcggatt gctctccatt tgtagtagaa
     2761 tacctaacta ttgcgaggcc tgcgaagccg tccgccgccg tgtagaagaa tatctcactt
     2821 gccgtagaag tacttcgaaa t
//
LOCUS       DATGFG010000573         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1028152, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000573 DATGFG010000000
VERSION     DATGFG010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028152"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VKQ52_10740"
     CDS             <1..230
                     /locus_tag="VKQ52_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87712.1"
                     /translation="DLSGTDLPHRYFEALCFICPFAVSAMAGPENQEWLNKLWDYIVN
                     FKLKDFDYYDNSIKMINMIILSGNYWAPLNG"
     gene            complement(232..1371)
                     /locus_tag="VKQ52_10745"
     CDS             complement(232..1371)
                     /locus_tag="VKQ52_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ87713.1"
                     /translation="MNKMSNKRSSIILLTATALLLAGMLSSCSSSDGEEKAKSTAEPA
                     KKDSSAAPTATFQLEKGQLSTDLFVPGELIAFQQVDLYAKVNSFVKKLYVDVGSEVKE
                     GQLLATMEAPELQSQLASAESRVKSQEAIYIASKANYDRVVETSKTPGTISQNDVDQA
                     DARQKSDMAQWDAAKSAYKEVVETLKYLEIRAPFSGVISMRNVNPGAYVGPSGKGSEL
                     PLFTLQEQGHLRLVVSVPEVLTGYLRDKQEVKFTVKSMPDHKFTATVRRLAGALDNRL
                     RAERTEMDVFPKNKELLPGMVAEVDLSMPARDSTYVVPASAVVNSTERVFVIRVTADR
                     KAEWIDVKTGRSQGGKTEVYSGKLGAGDQLVRTASEEIRNGAAIR"
     gene            complement(1352..>4038)
                     /locus_tag="VKQ52_10750"
     CDS             complement(1352..>4038)
                     /locus_tag="VKQ52_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ87714.1"
                     /translation="PDQLVEALRNNNQATPSGNVRIGNLNYFTPTNTTIATVKDFGDI
                     PIYVNGVQNVYLKDVAVVEDAADITAGYALINGKRSVYLPITKSAAASTWEVVQNLKK
                     AIPKFQAQLPEDVKLSYEFDQSVYVINAVRNLLSEGAIGALLTGLMVLLFLGDLRGAG
                     IVILTIPICVISGVLFLNLFHQTINIMTLSGLSLAIGILVDESTVTIENIHQHLAMGK
                     PKSLAIWDACREIAFSKLLILFCILAVFAPAFTMSGIPGALFLPLALAIAFSMITSYL
                     MAQTFVPVMANWVMKGHHKKGGAGFASDEEEFRASGLTAADEKNVAEEKQKLMEHGTG
                     KDGKETRFDRFRKRFLRFTDRMMPYRMPIAIAYIVLGLGGAWLLLNSIGRDVLPRNNA
                     GQFQVRLRAPDGTRIERSEEYMIRTLHILDTMVGHENIGVTSAMVGMHGGQFSTNPIY
                     LFMAGPQEGVLQVSLKEDYKVKMDDLKEEFRRRLAQDLPEIKVSFEPIELTDKILSQG
                     SPTPIEVKLIGKNKKINEQYAYKVIERLKQIPYLRDVQLGQAIHYPAVNIDIDRIRAA
                     QLGANAAAISRSLLAATSSSRFTEKNVWLDHTSNLSYFVQVQVPENKMTSLHDIGETP
                     IVPNLPRPQLSDVATLRVDSVYGEDDDIGATPVLSVTANLNKIDLGRATHDVNEAIGS
                     IGKLPRGLTIETRGLSQVLTETLDSLQTGLITAVIVIFLMLAANFQSFKVSLVTLCTV
                     PAVLLGSLALLMLTGSTLNLQSYMGMIMSVGVSISNSVLLVTNAEELRMHSGDALKAA
                     REAAATRLRPILMTSVAMVVGMIPMASGLGEGGDQAAPLGRAVIGGLIASTFAALFFL
                     PLIFAWVQRKTTTQSVSLDPSDKVSKHYIPSLYEQNEQ"
BASE COUNT          846 a         1232 c         1081 g          879 t
ORIGIN      
        1 atgatctttc cgggacggac cttccccacc gctatttcga agcgctgtgc ttcatctgcc
       61 cctttgccgt ctccgcgatg gccggccccg aaaaccagga atggctcaac aaattgtggg
      121 actatatcgt caacttcaaa ttaaaggact tcgattacta cgataacagc atcaaaatga
      181 tcaatatgat catcctctcc ggcaattact gggctccgct gaacggatag cctaacggat
      241 tgccgctcca ttccgtattt cttcactggc cgttcttacc agttgatcgc ccgcacccaa
      301 cttgccgctg tatacttctg tctttccccc ctgtgacctg ccggtcttca catcgatcca
      361 ttcggctttc cggtcggcgg tgacccgtat gacaaagacc ctctccgtcg agttgacgac
      421 ggcggaagcc ggcaccacat acgtgctgtc cctcgcgggc atcgacaggt cgacctcggc
      481 caccatgccg ggcagcagtt ccttgttctt cggaaagaca tccatctccg ttctttccgc
      541 gcggagccgg ttgtccagtg cgccggccag cctcctgaca gtagccgtga acttatggtc
      601 cggcatggac ttcaccgtaa acttcacttc ctgcttgtcg cgcaggtacc ccgtcaatac
      661 ttcgggaacg gaaaccacga gccgcagatg cccctgctcc tgcagggtga agagcggcaa
      721 ctcggaccct ttaccggaag ggcctacata ggctccggga ttgacattac gcatgctgat
      781 gaccccgctg aacggagccc ggatctcgag atatttcaac gtctcgacca cctctttata
      841 ggccgatttt gcggcatccc attgcgccat atcggatttc tgccgcgcat cggcctggtc
      901 cacgtcattc tgggagatgg tgcccggcgt cttgctggtc tccacgaccc ggtcataatt
      961 ggccttgctg gcaatataga tggcctcctg ggatttcacc ctggattcgg cgctcgccag
     1021 ctggctctgc aattcgggcg cttccatcgt cgccagcaac tgcccttcct ttacctcgga
     1081 accgacgtcc acatacaact ttttgacaaa gctgttgacc tttgcataga ggtccacctg
     1141 ctggaacgcg atcaattccc cgggcacaaa gagatcggtg gacaactggc ccttctccag
     1201 ttggaaagta gccgtggggg cagccgaact atcctttttc gcgggctcgg ccgtactctt
     1261 cgccttttcc tccccatcgg aagaggaaca gctgctgagc ataccggcca gcaacaaggc
     1321 ggtcgcagtc agcaggatga tggatgatct tttattgctc attttgttca tacaatgacg
     1381 gtatataatg tttacttact ttatcggacg gatcaaggga tacggattgc gtggtcgtct
     1441 tgcgctgtac ccaggcgaag atcaagggca ggaagaagag ggccgcaaag gtcgaagcga
     1501 tgagtccgcc gatcacggcg cgaccgagcg gagccgcctg gtccccgcct tccccaagcc
     1561 cggaagccat cgggatcatc ccgacaacca tcgccacgct ggtcatcagg atcgggcgaa
     1621 gccgcgtagc tgccgcctcc ctggccgcct tcaacgcatc cccggaatgc atgcgcagct
     1681 cttcggcatt ggtcaccagc aacacggagt tggagatcga cactccaacg gacatgatca
     1741 tccccatata ggattgcagg ttgagcgtcg agccggtgag catcagcagc gccagtgaac
     1801 ccagcaacac cgccggtacg gtacagagcg tgacaagcga gaccttgaag gactggaaat
     1861 tggcagccag catcaggaag atgacgataa cggcagtgat aagcccggtc tgcaggctgt
     1921 ccagcgtctc cgtcagcacc tgtgaaagac cacgggtctc gatcgtcagc ccgcgcggaa
     1981 gcttcccgat ggatccgatg gcctcgttca cgtcatgggt cgcccgcccg agatcgatct
     2041 tattcagatt ggccgtcacc gacaatacgg gtgtcgcacc gatgtcgtca tcttccccgt
     2101 aaacggaatc aaccctcaac gtggcgacat cactcagctg cggtcgcggc agattgggta
     2161 cgatcggcgt ctccccgata tcgtgaaggc tcgtcatctt gttctcgggc acctgcacct
     2221 gcacgaaata ggaaaggttg gacgtatggt cgagccagac gttcttttcg gtgaaccggg
     2281 aagaagaggt ggccgcgagc aggctgcggc tgatggcagc ggcattggcg cccagctgcg
     2341 ccgcccgtat gcggtcgata tcgatgttca cggccgggta atggatggcc tggccaagct
     2401 gcacatcccg cagatacggt atctgcttca accgctcgat caccttgtag gcatattgtt
     2461 cattgatctt cttattcttt ccgatcagct ttacctcgat cggcgtcggc gaaccctggc
     2521 tcaggatctt atcggtcagc tcgatcggct cgaaggagac ctttatttcg ggcaggtcct
     2581 gcgccagccg gcgccggaat tcctccttca gatcgtccat ctttaccttg tagtcctcct
     2641 tcaggcttac ctgtaatacc ccctcctggg gacccgccat gaacaaatag atagggttcg
     2701 tggagaactg gcccccgtgc atccccacca tggccgaggt cacgccgata ttctcatgcc
     2761 caaccatcgt gtcgaggata tgcagggtcc ggatcatgta ttcttcggag cgttcgatcc
     2821 gcgtcccatc cggggcgcga agccgcacct ggaactgtcc cgcattattt ctgggcaata
     2881 catcccggcc gatactattc agcaggagcc aggccccgcc aagtcccagg acgatgtaag
     2941 cgatcgctat cggcatacga taaggcatca tccggtcggt aaaccggaga aagcgtttgc
     3001 gaaaccggtc gaaacgggtc tccttgccat ccttaccggt gccgtgttcc atcagcttct
     3061 gcttctcttc ggccacattc ttttcatcgg cagccgtcaa cccggaagcc ctgaactctt
     3121 cttcgtcgga agcaaagccc gccccccctt tcttgtgatg ccccttcatg acccagttgg
     3181 ccatcacggg cacaaaggtc tgggccatca ggtaggaagt gatcatcgag aaagcgatcg
     3241 ccagggccag gggcaggaac aacgccccgg ggatgccgct catcgtaaag gccggcgcaa
     3301 acaccgccag gatacaaaag aggatcagca gtttcgagaa agcgatctcc cggcacgcat
     3361 cccagatggc cagcgacttc ggtttgccca tcgccaggtg ctggtggata ttctcgatcg
     3421 tcaccgtgga ctcatccacc aggataccga tcgccaggga aagcccgctc agcgtcatga
     3481 tattgatggt ctgatggaac aggttgagga aaagaacacc cgagatcaca cagatcggga
     3541 tagtcaggat cacgatgccg gcgccacgga ggtcaccgag aaagagcaat accatcagcc
     3601 ccgtaagaag ggcgccgatc gccccttcgg acaacaggtt cctgacggca ttgatcacat
     3661 atacggactg gtcgaattca taggagagtt ttacgtcctc cggtagctgc gcctggaatt
     3721 tggggatcgc cttcttcagg ttctgcacca cctcccaggt ggaagcagcg gcagacttgg
     3781 tgatcggcag gtacacactc cgcttgccgt tgatcagcgc atatccggcg gtgatatccg
     3841 ccgcgtcttc caccaccgcc acatctttca ggtagacatt ctgcaccccg ttgacataga
     3901 tcgggatatc gccaaagtcc ttcaccgtcg caatcgtcgt gttcgtaggg gtaaaatagt
     3961 tgaggttgcc gatgcggaca ttccccgaag gggtcgcctg gttattgttc cgtaaggcct
     4021 ccaccagttg atccgggg
//
LOCUS       DATGFG010000574         6921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1474584, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000574 DATGFG010000000
VERSION     DATGFG010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474584"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VKQ52_10755"
     CDS             <1..573
                     /locus_tag="VKQ52_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HLZ87715.1"
                     /translation="EKVWRSIQQKDGQAFENFYREHYRFFLLAACKYLRDAGPAQEIV
                     NDVFIKLWEEAETIHIETSLKFYVHRAVVNRCLNVLDREKRDRERQRGLIHLPQEVSE
                     SREMEDDELKIRLYQAIDRLPEQCRKVFRMSRFEELKQREIADKLGISIKTVKNHITY
                     ALKHLNVVLGDWNRLPAWIGLINYFFWPHR"
     gene            611..1717
                     /locus_tag="VKQ52_10760"
     CDS             611..1717
                     /locus_tag="VKQ52_10760"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ87716.1"
                     /translation="MDPNATYDDLSWELIVLALQGELSPEDELRFHQWLASSAANREH
                     YEGLARIWKEGVADYPFYEQANETQGWVALRQKMEAGQAGWEEAGKAGLGEAAQAGRG
                     EAGQEGREEARVIPVGAGKRVIGMARRMAVAAVLLLMAGAGWWYLSGRGSATAYETAA
                     NEEKNIPLPDGSTVQLQPRTRIEVAAGYNKTDRTVVLIEGKARFDVSRQKQQPFMVDM
                     DAAQVKDIGTSFTIEKTKDSIEVAVSEGRIAFIPKKAGAPREISAGGVVCLYTPAHGL
                     GEIRETGSAAGSADSLRFDNAPLSTVLSALEKVSGKKILLTDAQIAQKKLTIRLSGES
                     LEDALRVICASFDLEYSVRNGVYILEKRKPSNHP"
     gene            1888..4395
                     /locus_tag="VKQ52_10765"
     CDS             1888..4395
                     /locus_tag="VKQ52_10765"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ87717.1"
                     /translation="MPEALAIVESRVAVRFAYPTELLRGQKNVSITASQMPLPIFLDS
                     LFLGTGLSYQVIGDQIVLQGLFPSSRVTLSGYIRDSLTGELLIGASVYLPATGAGVLS
                     NNYGFYSLSVPDADTIELEVSYVGYKSMRRRVNAKGDVSLSIGLPLNNVQEEINTLTI
                     VNDKREDFVKKNPSGLTDLSPEMIVAAPSVSGSGDLIGSVEMLPGVQAGIDGTPGYFV
                     RGGNAGQNLILLDEATLYNPSHIFGLVSVFNPPTIKHASLMKGGFPAVYGDHVSSVLD
                     VAMKDGSNQQTGGVIQMGSVSTGVTLYGPLEAGKSSFLVSGRRSTTDLLLHPFLHNNY
                     FSNYYFYDVNAKLNFEVSPRDRLLFSFYSGRDNNTYSSDSTDSAGIGYSMHFGNTAFT
                     ARWTHPFSGKVFSSTSVEYNRYHQFLSATQEGYFAQLYSGIRDINAKTNITWYPSPVH
                     KINAGVDYLYQTLYPASLSGRVAPADSTLTIVPSGIPPKTATRMAAYASDEIRWGRWL
                     VSMGVRLPYYYKPDAQYFSVEPRLSLLYMIDATTSAKLSFATMHQYIHLVQSYNATFP
                     AEIWIGSSNVVRPQASRELSAGLFKNFSGNTFQTSVEFYYKRLDNQLLFGGQATPVID
                     NSIESELIFGKGWDYGAELFLRKNRGKWTGWLAYTLAYAYEQFDSLNEGQRFPFAYDR
                     RQMLDISAAYSFAPHWKVAANLLLASGRAFSLSPDSTFVPGNDPLYANPGRGRGRGRG
                     RGGNPNVDTGTWNIIANNYRLSPYNRLDLSVHYARTRTTAKRVTETEWIFSVYNVYAR
                     PNNAFVYRTIDPATRKVIARELPLIPVIPSITYSYKF"
     gene            4514..5326
                     /locus_tag="VKQ52_10770"
     CDS             4514..5326
                     /locus_tag="VKQ52_10770"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HLZ87718.1"
                     /translation="MKSTNTRRIIWLFVPLAAGILIFAACKKSSSSGGNGQLEVRLTD
                     GPSPDYDAVLIDVEKVEVNVSADTGTGSGWQTLPLLKPGIYNLLDFRNGIDTVLASVS
                     LPAGTLSQMRLTLGSNNSVVINGQSYPLKTPSAQQSGLKFNIHSTLTSGIVYRLWIDF
                     NAGRSIVTTGNGDFILKPVIRTYSEAIGGSIKGTVLPTAAKPTVWAIQGTDSLLALPD
                     SATGYYFFGGVNAGSWNLLFHAQDTTYKDTAFTVTVSTGVVTNAGTVTLRKK"
     gene            complement(5436..5693)
                     /locus_tag="VKQ52_10775"
     CDS             complement(5436..5693)
                     /locus_tag="VKQ52_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87719.1"
                     /translation="MDPDHSTPDDLKASILSDIQAFLISLEAGASNEQLDASLTRIRE
                     REILLLKEKGLMLSPVLWKIMHSRFANKKDKDIIDTTGYGQ"
     gene            5797..6171
                     /gene="crcB"
                     /locus_tag="VKQ52_10780"
     CDS             5797..6171
                     /gene="crcB"
                     /locus_tag="VKQ52_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HLZ87720.1"
                     /translation="MKTAMIIALGGALGSLSRYGVQTFVYRVYPFVFPFGTFLVNVAG
                     CLLIGMIYGLSEKGNLLSPEWRLFLTTGFCGGFTTFSTFAYENANLVQGSEYLYTALY
                     ILGSVVLGIGAVFLGILLIRIV"
     gene            6183..6536
                     /locus_tag="VKQ52_10785"
     CDS             6183..6536
                     /locus_tag="VKQ52_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HLZ87721.1"
                     /translation="MELNGDSKLLRIFIGEADKIGHQCLYEAILMAAKERGLAGCTVM
                     RGIMSFGASSRVHAARLIEISRDLPIVVEIVDKEEKVTAFLEIAGSLLEKAGCGGLIT
                     IEKAQVLYYKPAPGK"
BASE COUNT         1591 a         1780 c         1952 g         1598 t
ORIGIN      
        1 gaaaaagtct ggcgatccat acaacaaaag gatgggcagg catttgaaaa tttctaccga
       61 gagcactatc gtttctttct gctggctgcg tgcaagtatc tcagagacgc ggggccggcc
      121 caggaaatcg tcaatgatgt ctttatcaaa ctttgggaag aggcggaaac catccatatc
      181 gagacctcgt tgaagttcta tgtccaccgt gcggtggtca accggtgcct taacgtcctg
      241 gacagggaaa aaagagacag ggagcgtcaa cgggggctaa tccacctccc gcaggaggtg
      301 tccgaatcgc gggagatgga ggacgatgag ctgaagatca ggctgtacca ggccatcgac
      361 cgattgccgg agcagtgccg gaaggtattt cggatgagcc gttttgagga attgaagcag
      421 cgggagattg ccgataagct ggggatctcc atcaaaaccg ttaagaatca tataacttat
      481 gcgctgaagc acctgaatgt ggtgcttggc gactggaatc gtctgccggc atggatcggc
      541 ctgataaatt attttttttg gccccaccgc taggtctgtg ggcgcttttt tttgtcttat
      601 atctgagaat atggatccga acgcaacata tgatgatctt tcctgggagc tgatcgtgct
      661 ggccctgcaa ggtgaattgt cgccggaaga cgagctccgc ttccatcagt ggctggcatc
      721 ctcggcggct aaccgggaac attatgaagg cctggcgcgg atctggaagg agggtgtggc
      781 agattatccg ttttatgagc aggcgaatga aacccagggc tgggtagcgc tccggcaaaa
      841 aatggaggcc gggcaggcgg gttgggaaga agccggaaag gcaggcttgg gagaggcggc
      901 gcaggcgggc cgtggagagg cggggcaaga aggccgggaa gaagccaggg tcatccctgt
      961 tggcgccgga aagcgggtga tcgggatggc gcgccggatg gccgtggccg ctgtgctatt
     1021 gttgatggcc ggggccggat ggtggtatct ctccggaagg ggatctgcca ccgcttacga
     1081 gacggcggcc aatgaagaaa agaatattcc gctgccggac gggtctactg tacaactaca
     1141 gccccggacc cggatcgagg tagctgccgg atacaataag acggaccgga ccgtggttct
     1201 tatcgaagga aaagcacggt ttgacgtatc ccgtcagaaa caacagccct ttatggtgga
     1261 catggatgcc gcccaggtaa aagacatcgg gaccagcttt accatcgaaa agacgaaaga
     1321 cagtatagag gtggcggtat ccgaaggcag gatcgctttt attccgaaaa aggccggtgc
     1381 gcccagggaa atatccgcgg gcggcgttgt gtgtttgtat actccggcac atggtttggg
     1441 agagatccgg gaaacgggtt ctgccgcggg aagcgccgat tcattgcgat tcgacaatgc
     1501 gcctttgtcc acggttctca gcgccctgga aaaggtctcc ggcaagaaga tcctgctgac
     1561 cgatgcgcag atcgcacaaa aaaagctgac gatccggctg agcggagaat ccctggaaga
     1621 tgcgcttcgg gtgatctgtg cgtccttcga ccttgaatac agtgtgagaa acggagtata
     1681 tatcctggaa aaacgaaaac cttcgaacca cccttaaaca gactactcat ccataccaat
     1741 cagcgcatta ccggcgttta cgcagcgtta ccaacctttg ccctggcgtt ggtatgcctt
     1801 ctattccctt cgttattcta tccggctgcc gcccagcaaa agccggggcc ggagcccctg
     1861 gtgacggtcg acatcaaaaa caggccgttg cccgaagccc tggccatcgt cgaatcccgc
     1921 gtcgccgtcc ggttcgccta tccgacggaa ctgttgcgcg gacagaagaa tgtttcgata
     1981 acggcctcgc agatgccgct gcctatcttc ctggatagtc ttttcctggg gaccggtctt
     2041 agttaccagg tcatcggcga ccagatcgtc ctccaaggcc ttttcccatc gtcgagggtc
     2101 acgctcagcg gttatatccg ggactccctg acgggggaat tgctcatcgg ggccagcgtt
     2161 tatctcccgg cgaccggggc gggtgtgctt tccaataatt atggctttta ttcgcttagc
     2221 gtcccggatg ccgataccat cgagctggag gtttcctacg tggggtataa aagcatgcgg
     2281 cggcgggtga acgcgaaggg ggatgtttct ctttccattg ggctgccact aaacaatgtg
     2341 caggaggaga tcaataccct aaccatcgtc aatgacaaac gggaggattt tgtaaagaag
     2401 aacccgtccg gtttaacgga cctctcgcca gagatgatcg ttgcagcccc ttcggtcagc
     2461 ggcagcgggg atcttatcgg cagtgtggag atgctccccg gagtgcaggc gggtatcgac
     2521 ggaacgccgg gctattttgt caggggcgga aatgccgggc agaacctgat cctgctggac
     2581 gaggccaccc tgtataaccc cagtcatata ttcgggctgg tgagtgtttt caatccgccc
     2641 accatcaaac acgccagcct gatgaaaggg ggctttcccg ctgtttatgg cgatcacgtg
     2701 agctcggtgc tggatgtagc catgaaggac ggcagcaatc agcagaccgg aggggtcatc
     2761 cagatgggaa gtgtttcaac cggggtaacc ctttacggcc ccctggaagc cggcaaatcg
     2821 tcctttctcg tttccgggcg caggagtacg accgatctgt tgcttcaccc cttcctccat
     2881 aacaactatt tcagcaatta ttacttctat gatgtaaacg cgaagctcaa tttcgaggtt
     2941 tcgcccaggg acaggctcct tttcagcttt tacagcggta gggacaacaa tacttactcc
     3001 agcgacagca cggacagcgc ggggatcggt tattccatgc atttcggcaa tacggccttt
     3061 accgcgcggt ggacccatcc cttctcggga aaagtatttt ccagcacttc ggtggaatac
     3121 aaccggtatc accagttcct gtcggctacc caggagggtt attttgcgca gttgtattcg
     3181 gggatcaggg atatcaatgc caagacgaat attacgtggt atccctcccc tgttcataag
     3241 atcaatgcgg gggtggacta tctctatcaa acgctctatc ccgcatcgtt gtcggggcgg
     3301 gtggcgccgg cggattcgac gctgaccatc gtgccgtcgg ggataccacc caaaacggcc
     3361 acgcgcatgg ctgcttatgc cagtgacgag atcaggtggg ggcgctggct ggtatcgatg
     3421 ggggtgcgcc tgccctatta ttacaaaccc gatgcgcagt atttttcggt ggagccgaga
     3481 ttgtccctgc tgtacatgat cgatgcgaca acgagcgcga agctgtcctt tgccaccatg
     3541 catcagtata tccatcttgt gcaaagctat aatgccacct tcccggccga gatctggatc
     3601 ggcagcagca atgttgtgcg gccgcaggca tcccgggaac tgtcggcggg gctgttcaag
     3661 aacttttcag gaaatacctt ccagaccagt gtcgaattct attataagcg cctggacaat
     3721 caattgttgt tcggaggcca ggcgacgccg gtgatcgaca acagtatcga gagcgaactc
     3781 atcttcggca agggatggga ctatggcgcc gagctcttcc ttcgcaaaaa cagggggaaa
     3841 tggaccggat ggctcgccta tacgcttgcc tatgcctatg aacagttcga ttcgctgaac
     3901 gagggccagc gtttcccgtt tgcctatgac agaaggcaaa tgctcgatat ctcggcggcc
     3961 tattcgttcg cgccgcactg gaaggttgcc gccaatttgc ttttggcgag cgggcgggca
     4021 ttttccctca gcccggattc cacctttgtt cccggcaatg atcccctgta tgccaatccc
     4081 gggagaggcc gggggcgcgg ccggggaagg ggcgggaatc cgaacgtgga taccggcacc
     4141 tggaatatca ttgccaataa ctaccggctt agtccttata atcgcctcga cctgagtgtg
     4201 cattatgcca ggaccaggac caccgccaaa agggtaaccg agacggaatg gatcttttcg
     4261 gtgtataatg tgtatgcccg gcccaacaat gcctttgttt accggacgat cgatcccgcc
     4321 accaggaagg tgatagccag ggaattgcct ctcattcctg tcattcccag tattacatat
     4381 agctacaaat tttaattcaa acgtaaaaaa agggtccctg cactgggacc aggggtgcgt
     4441 tttcctgtct tatcagggaa gaaccggatg accgtcagca aatggcgggg atcctcaaac
     4501 aaaaatcaca ctcatgaaaa gtacaaacac acgcagaatc atttggttat tcgttccgct
     4561 ggccgcaggc atcctgatct ttgccgcctg caaaaaaagc agcagcagcg gtggcaacgg
     4621 gcaactggaa gtacgattga cggacggccc ttcgccggac tatgacgcag tactcatcga
     4681 tgtcgaaaag gtagaagtga acgtgtccgc cgacaccggg accggttcgg ggtggcagac
     4741 gttgccgctg cttaagcccg ggatctataa cctgcttgat ttcagaaatg gcatcgacac
     4801 ggtcctggcg tccgtgagcc tgcctgccgg gacgctgtcg cagatgcggc tgaccctcgg
     4861 gagcaataat tccgtggtca taaacgggca atcttatccc ctgaagactc cttccgcgca
     4921 gcaatcgggt ttaaaattca atatccacag cacgctgacc agcggcatcg tttaccggct
     4981 ttggatcgat ttcaatgccg ggcgcagcat cgtgacaacg ggaaacggcg atttcatcct
     5041 taagccggtg atccgtactt attccgaggc tattggcggc agtatcaagg gaactgtact
     5101 gcctaccgca gccaagccga cggtttgggc gatacagggc acggatagcc tgctggcgtt
     5161 gcccgattcg gcaaccggct attatttctt cggcggcgtg aatgccggtt cgtggaattt
     5221 actcttccat gcacaggata ccacctataa agacactgct ttcacggtga ccgtcagcac
     5281 gggcgttgtg acgaatgccg ggacggtgac gcttcgcaag aaataacacc tatgattacc
     5341 tgtataaatt aagtactccc aagtctatcg agatcatctc gtaggatttt ggattttagc
     5401 acaacggccc ggatctctat ccgggccttt ttgttttact gcccgtatcc agtcgtatca
     5461 atgatgtcct tgtctttttt attggcaaaa cggctgtgca tgatcttcca taataccggt
     5521 gagagcatga gtcctttttc ttttaacagt agaatttccc gctccctgat gcgggtgaga
     5581 ctggcatcca gctgttcgtt ggaagcaccg gcctccaggc tgatgaggaa tgcctggata
     5641 tcagagagga tggatgcttt cagatcgtcg ggagttgaat gatcagggtc catatggtcg
     5701 gtgccttaac agagttgcca taagcgcagc aaaaaatgca ccaattattg taactttaag
     5761 acctcacctg cacctatcga tgaaaaaagt aaatcgatga agacggccat gatcatcgcc
     5821 ctgggcggcg ccctcggcag cctgtcccgt tacggcgttc aaacctttgt ttacagggta
     5881 tatccattcg tcttcccgtt tggtaccttt cttgtcaatg tcgccggatg cctgcttatc
     5941 gggatgatct atggtctttc cgagaaaggc aatctcctgt cgcccgaatg gcgccttttt
     6001 cttaccaccg gcttttgcgg cgggttcacc accttctcca ctttcgccta tgagaatgcc
     6061 aatcttgtcc agggttccga gtacctgtat accgcgcttt atattctcgg tagtgtcgtc
     6121 ctgggtatcg gcgcggtgtt cctgggtatc ctcctcatcc gaatagtata aaacgcctgt
     6181 ctatggaact gaacggagat tccaaactat tgcgcatctt tatcggagaa gccgataaga
     6241 tcggtcacca gtgtctttat gaagccatcc tgatggcggc caaggagcgg ggccttgcgg
     6301 gttgtacggt catgcggggg atcatgtcct tcggcgcctc ttcccgggta catgccgcgc
     6361 ggttgatcga gatctcccgg gatctgccca ttgtcgtgga gatcgtggac aaagaagaaa
     6421 aggttaccgc ctttttggag attgccggtt ccctgctcga aaaagccggc tgcggcggtc
     6481 ttattactat tgaaaaggcc caggttctgt actataaacc ggcgcccggc aaatgaccgg
     6541 aagggacgga agggcgttaa gggcagtgcg gggagctcaa ttaagcatgg ctgaccagct
     6601 gatgaacaca ccagacaaac aggcaaccaa aaatcatccc gagggtgaaa aggatgagga
     6661 ggcctttaac agaataagca cgcttgtttc tatctatttg atagtcagcc aatttcaaat
     6721 aaatgaggtt gaaccggggt taaaattgga ttaaaattag attaagaaat cccaataagc
     6781 aaacttaggg tcatgaaagt tgccattgca ctatcgatcg ccgttttgca cgtgctgtcc
     6841 tcccgggagc aggtgcttcc cctgaaggtg agcggtgatc accattttct cgtaaccacg
     6901 gatgggaaac catttttctg g
//
LOCUS       DATGFG010000575         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1621347, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000575 DATGFG010000000
VERSION     DATGFG010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621347"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2011)
                     /locus_tag="VKQ52_10790"
     CDS             complement(<1..2011)
                     /locus_tag="VKQ52_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HLZ87722.1"
                     /translation="MSDKKYWQSFGERSNSEGYRKATGDEFQESLPLEGLDGKGLLDA
                     KHPRRDFLKYLGFSTAAATLAASCEMPVRHVVPYLNKPDNLIPGVADYYATTYTNGGD
                     VVSVVAKVRDGRPIKLEGNTLSSLSKGGTSARVQAAVLDLYDMARLRFPVQMTDGKAA
                     EVSTLEAFDKLVGEALAAAGGPVVLLTGTLTSPTTKQIISEFLAKYPGSRHVQYDADS
                     YSGILLANEASYGKRAIPSYRFDNAKVVVSLGADFLGTWLTPVDFARQWSVNRKVSAN
                     NPEMSKHYQFESLLSMTGANADERITHRPTETGAIALALLAELGGSVTAPALPEKVKA
                     GVKKAAKDLMDNKGKALVVSGSNNVNVQIIVNAINEAIGAGGSTINWNAPIQVRQGID
                     SEMNALVADLNDGKVGALLVYGVNPAYDYYNAEAFKSGLKKVKASISFNDRLDETTEL
                     TKFVLPAPHFLESWGDAEPKPGYFSFLQPTIAPLFKTRHFQDSLMKWSGSTTTYEAFF
                     KQYWTSKLGSVEAYEKALQDGVIEPATEAASGVSYNNSKVGDAASAISAIKKGGAFEV
                     VLYQKVTMGTGAQANNPWLQEVPDPVTKADWDNYAVVSPKFAKDTWNLDLGDRRQADA
                     YEVYPTKQVIKVKAGNKELSLPAVIIPGTNNEVIGIAVGYGRSSAN"
BASE COUNT          443 a          607 c          639 g          534 t
ORIGIN      
        1 ggttggcgct gctccggcca tagccgacgg cgataccgat cacctcgtta ttggtgccgg
       61 ggatgatcac cgcgggaagg ctcagttctt tgttgccggc ttttaccttg atcacctgct
      121 tggttggata gacttcgtaa gcatcggcct ggcggcggtc gccaagatcc aggttccagg
      181 tatccttggc gaatttgggg gatacgacgg catagttgtc ccagtcggcc ttcgtcaccg
      241 gatcgggtac ttcctgcaac caggggttgt tggcctgtgc gccggtaccc atcgtcacct
      301 tctggtaaag gacaacctca aaggcgccgc ctttcttgat ggcgctgatg gcgctggcgg
      361 catcacccac cttcgagtta ttatacgata cgccggaggc ggcttccgtg gcaggctcga
      421 tgaccccgtc ctgcagggcc ttctcgtagg cttccacgct gcccagcttg ctggtccagt
      481 attgtttgaa aaaggcttcg taagtagtcg tgctcccgct ccatttcatc agggagtcct
      541 ggaaatgtct cgtcttgaac agcggggcga tcgtcggctg aaggaaggag aaatagccgg
      601 gcttgggttc ggcatccccc cagctctcga ggaagtgcgg ggcaggaaga acgaatttgg
      661 tgagctcggt cgtttcgtcg agccggtcat tgaaggagat cgatgccttc acttttttca
      721 ggccggattt gaatgcctcg gcattataat aatcataggc cgggttgacc ccgtaaacaa
      781 gcagcgcgcc tactttcccg tcgttgagat cggcgacaag ggcgttcatc tcgctgtcga
      841 taccctggcg aacctggatg ggggcgttcc aattgatggt agacccgccg gcgccgatgg
      901 cttcgttgat ggcgtttacg atgatctgga cattgacatt attgctgccg ctgacgacca
      961 gggccttgcc cttattgtcc atcaggtctt ttgctgcttt tttaaccccg gccttcacct
     1021 tttccggaag agcaggggcc gtaacggatc cacccagctc agccagcagg gccagcgcga
     1081 tcgcgcccgt ttcggtgggg cgatgggtaa tgcgttcgtc ggcgttggcg ccggtcatcg
     1141 agagcaggct ttcaaactgg tagtgcttgc tcatctcggg gttgttggcg ctgaccttcc
     1201 ggttcaccga ccactgacgg gcgaagtcca ccggcgtcag ccaggttccc aggaagtccg
     1261 ctccaaggga gacgaccacc ttggcattgt caaaccggta ggacggaatg gcgcgtttgc
     1321 cgtaggaagc ctcatttgcc agcaaaatac cgctataaga atcggcatcg tactgtacat
     1381 gacggctgcc cggatatttt gccaggaact cggaaatgat ctgtttggtc gtgggagaag
     1441 taagcgtacc ggtcagcagc acgacgggac caccggccgc ggccaaggcc tcaccaacca
     1501 gcttgtcaaa agcttcgagg gtgctgacct cggcggcctt gccatccgtc atttgaacag
     1561 ggaaccgcag ccgggccatg tcataaaggt cgagcacggc ggcctgcacc cgcgccgatg
     1621 taccaccctt gctaagagaa gaaagggtgt tgccttcgag cttgataggg cggccatccc
     1681 tgactttcgc aacgacggaa acgacatcac caccattggt ataggtggtt gcataatagt
     1741 cggcgacgcc gggaatgagg ttatccggtt tgttgagata gggaacaaca tgacggacag
     1801 gcatttcgca ggaagcagcc agggtggcgg cagccgtgct aaagccaaga tattttagaa
     1861 aatccctgcg gggatgtttg gcatcgagca atccctttcc gtccagcccc tccagcggta
     1921 agctctcctg aaactcatcc ccggtcgcct tccgataccc ctcgctgtta ctacgctctc
     1981 cgaaactttg ccagtatttt ttgtcgctca tattggagaa gttatatata aattgtcttc
     2041 caaatcggtt ttacttactc cgcgggattt ctctttcgac taaatcgcgg tcacaccgcg
     2101 gaggtgggat taataatggc atttctggca ttcggtaccg ccgatgtcct tgacagtgat
     2161 gctgtccttg tcatgattct tcaggtcatc gtggaaatgc tcgtaaatgc tatagaactt
     2221 att
//
LOCUS       DATGFG010000576         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1034697, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000576 DATGFG010000000
VERSION     DATGFG010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034697"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VKQ52_10795"
     CDS             complement(<1..270)
                     /locus_tag="VKQ52_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87723.1"
                     /translation="MKKFLFWAASCCLLSSCNNAGTGSSTKDTTAAATATPATPPQSE
                     FADARYTEMGKKALAQLSSGDVTGWMDAYADNAVYIWSSGDSLAGK"
     gene            complement(311..874)
                     /locus_tag="VKQ52_10800"
     CDS             complement(311..874)
                     /locus_tag="VKQ52_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87724.1"
                     /translation="MKLNPAVALLVVLAACNSAPSAPATDASKPAVDSAAMKAIRSPY
                     DIRYSSKFEMDDPKNAETVLALWKAYDNGDLNVGKDMIADSIALYLANGAAIHTSRDS
                     ATASVQAVRNSFKAVEDRVDAVMAIKSTDRNEHWVLIWGTEVDTHKDGKIDSTQLQET
                     WRFNSSGKADLLYQFARPPLPPAPKKK"
     gene            1010..2575
                     /locus_tag="VKQ52_10805"
     CDS             1010..2575
                     /locus_tag="VKQ52_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87725.1"
                     /translation="MTRIHLYLLPLVFAALPAQTFAQPKAAPATTMAQPRSAPGAPSA
                     ERPVSGSRILNIDSAVTTRAEVTIKGQHVPYEATAGTIPVWDEEGKPIAGVFYTYYER
                     SDIKDRDARPLVISFNGGPGTPSVWMEIGYTGPRRLKIDDEGYPIQPYGVQENPYSIL
                     DVADIVYIDPVNTGFSRAVSKDIPTSKFFGVNEDIKYLAEWIATFISRKNRWASPKFL
                     IGESYGTTRVSGLALELQDAQWIYLNGVVLVSPTDLGIGRSDFVKTSLLVPYYAATAW
                     YHKALAPDLQKKDLTDYLPEVEDFTVNELIPALAKGGFIDEPKRQALATKLARYIGLS
                     EKAVLQSNLEIPTSFFWKELLRDKGFTVGRLDSRYKGIDRQDAGAQPDYNAELTSWLH
                     SFTPAINIYLREDLHYHTDLKYFMFGPVWPWDNQNNHVGEELRQAIAQNPYLHLLIQS
                     GYYDGACDYFNAKYSLWQLDPSGKLKDRLHWEGYRSGHMMYLRKPDLEAANESLRKFI
                     HEATPKPGEPAKY"
     gene            2666..>3602
                     /locus_tag="VKQ52_10810"
     CDS             2666..>3602
                     /locus_tag="VKQ52_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87726.1"
                     /translation="MPKPVLAIGLLALVFFGACKKDHSVSPFTFTIKLVSGGGQTDTI
                     GNVLKNQLSFLATQGNGAVRDGYLRFETMTANNTIQYTDYHLQNSPQANPIPLFNYAW
                     QLNQTVGTQTLKVILFDSVMEPKDSLTVNATALATSAGWHITSSIPIGTFCVAFGQLP
                     SGRVFTATNKQDYPYYSDDDGGTWHAITTFPMTAHITSIAVSKDNEVFMAVPNIGVYY
                     SQDKGRSWQLRDNNLPLQNYGGSLQCTASGTLLLETVSGPYYSTDKGQTWNWDGQGLL
                     NGAGPAPELPVTAFSMTDGTILAALNGYVVKSTNGG"
BASE COUNT          857 a         1226 c          801 g          718 t
ORIGIN      
        1 cttcccggca agactgtccc cggaagacca gatatacacg gcattatccg catacgcatc
       61 catccagccc gtgacatctc cggacgaaag ttgagccaat gccttcttac ccatctccgt
      121 ataacgggca tcggcaaatt cggattgcgg tggagtagcc ggggtcgccg tggcggcagc
      181 agtggtatct tttgtcgaag atccggtacc ggcattgttg caggaagaca gcaggcagca
      241 ggaagccgcc cagaagagaa actttttcat ggcaaagcat tttttttgat gaagtaaaag
      301 aagacagttg ctattttttc ttcggagccg gcggcagcgg tggcctggcg aattgataaa
      361 ggagatcggc cttcccgctc gagttgaatc gccaggtctc ctgcagttgc gtggaatcga
      421 tcttcccgtc cttatgggta tccacttccg tcccccagat cagcacccaa tgctcattcc
      481 tgtccgtgct cttgatggcc atcaccgcat ccacccggtc ctcgacggcc ttgaacgagt
      541 ttctaacggc ctgcacagac gcagtggcgc tatccctact ggtatggata gccgcaccat
      601 tcgccaggta aagcgcgatc gagtcggcga tcatgtcctt acccacattc agatcaccat
      661 tgtcataggc cttccataag gcaagcaccg tctcggcgtt tttcggatca tccatctcga
      721 acttcgatga atacctgatg tcataaggtg atcggatcgc tttcatggct gcggaatcca
      781 cggccggctt cgaagcatcg gtagccggcg ccgatggagc gctgttgcat gcggcaagaa
      841 caaccaggag ggccactgcg gggtttagtt tcataaccat aatttttaat taataaatta
      901 atgacgacca ataaggtacg attatttttt taatatataa catctaaacc cttacttcct
      961 cgtcctcgcg attttttcct atattcggtc attcgcataa actaactgta tgacccggat
     1021 tcacctttac ctcctcccgc tcgttttcgc cgccctcccg gcccaaacct ttgcccagcc
     1081 aaaagccgca cccgcaacca ccatggccca gccaagatcc gcaccaggcg cgccctccgc
     1141 cgaacgaccc gtcagcggaa gccgcatcct caatatcgat tccgccgtca ccaccagggc
     1201 cgaagtaacg atcaaaggcc agcacgtgcc ctacgaagcc accgccggca ccatccccgt
     1261 ctgggacgag gaaggcaaac ccatcgccgg ggtattctac acctattacg aacgctcgga
     1321 tataaaggac cgtgacgccc ggccactcgt gatctccttc aatggcggcc ccggaacccc
     1381 ctccgtatgg atggagatcg gctacaccgg cccccgccgc ctgaagatcg acgacgaagg
     1441 ataccccatc cagccctatg gcgtgcagga aaatccctac tccatcctgg atgtcgccga
     1501 catcgtatac atcgaccccg tcaataccgg cttttcccgc gccgtcagca aggatattcc
     1561 caccagcaaa ttcttcggcg tcaacgagga cataaaatat ctggcagagt ggatcgccac
     1621 cttcatctcc cgcaagaacc gctgggcctc ccccaaattc ctcatcggcg aaagctatgg
     1681 gaccacccgc gtctccggcc tcgccctcga attgcaggac gcccaatgga tctacctcaa
     1741 cggcgtagtc ctggtctccc cgacagacct tggcatcggc cgcagcgatt tcgtaaagac
     1801 ctccctcctc gtaccctact atgcagccac ggcctggtac cacaaggccc tcgcccccga
     1861 tctccaaaag aaagacctca ccgactacct ccccgaagta gaggacttca ccgtcaatga
     1921 gctgatcccc gccctcgcaa aaggcggctt catcgacgaa ccgaaaagac aggccctcgc
     1981 cacaaagctc gcccgctaca tcgggctatc tgaaaaagcc gtactgcaat ccaacctcga
     2041 gatccccact tccttctttt ggaaagagct cctccgcgac aaaggcttca ccgtcggcag
     2101 actcgactcc cgctacaaag gcatcgatcg ccaggacgcc ggcgcacaac ccgactacaa
     2161 cgcggaactc acctcctggc tgcactcctt cacccccgcc atcaatatct acctccggga
     2221 agacctgcac taccacaccg acctgaaata ttttatgttc ggccccgtct ggccatggga
     2281 caaccaaaac aatcacgtcg gcgaagagct ccgccaggcc atcgcccaaa acccctacct
     2341 ccacctgctg atccagtcgg gttactatga cggcgcctgc gattacttca acgccaaata
     2401 cagcctctgg caactcgacc ccagcggcaa actcaaagac agacttcact gggaaggcta
     2461 tcgcagcggc cacatgatgt acctgcgcaa acccgatctc gaagcagcaa acgaaagcct
     2521 gcgaaaattc atccacgagg ccacgccaaa acccggcgaa cccgcgaaat actgacgagc
     2581 gcacaaacct tcaagccgcg atcccatact gcttcgcccg cttgcatttc aacccttttc
     2641 cccctatctt gggaaaaact ttcctatgcc taaacctgtc ctggcgattg gcttgcttgc
     2701 cctcgtcttc ttcggcgcct gcaaaaaaga tcatagcgtc tcccccttta ccttcacgat
     2761 caaattggtg agtggtggcg gtcagaccga caccatcggc aacgtactaa agaatcaact
     2821 gagcttcctc gcaacccagg gtaacggagc cgtaagggac ggctacctcc gcttcgaaac
     2881 gatgactgcc aataacacca tccagtacac cgattatcat ctccagaact ccccacaggc
     2941 caatcccatc ccgctcttca attacgcctg gcagctcaac cagaccgtcg gcacccaaac
     3001 actaaaagtc attctcttcg attccgtaat ggaaccaaaa gattccctca ccgtcaacgc
     3061 caccgccctg gctacttccg cgggctggca tatcaccagc tccataccca tcggcacatt
     3121 ctgcgtagcc ttcggccagc tgccctccgg ccgggtattc actgctacca acaagcagga
     3181 ctacccctac tactcggacg atgacggcgg cacctggcac gccataacca ccttccccat
     3241 gacggcccat atcacctcca tcgccgtctc aaaagacaac gaggtcttca tggccgtccc
     3301 caacatcggc gtctattatt cccaggacaa gggccgatcc tggcagctgc gcgacaacaa
     3361 tctgccgtta caaaactatg gcggttcctt gcaatgcacc gcctccggta ccctgctcct
     3421 cgagaccgtc agcggcccct actactccac cgacaaggga caaacctgga attgggatgg
     3481 ccagggactg ctcaacggcg caggccccgc accggaactt cccgtcaccg ccttctcgat
     3541 gaccgacggt accatccttg cagccctgaa cggctatgta gtaaaatcca cgaacggtgg
     3601 ag
//
LOCUS       DATGFG010000577         5564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_983490, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000577 DATGFG010000000
VERSION     DATGFG010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983490"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VKQ52_10815"
     CDS             <1..1053
                     /locus_tag="VKQ52_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ87727.1"
                     /translation="LSVTDVGLKEIGSGVRGSNDFQELGDLNPSIRSIVAYNTDSKII
                     GTLRANGILLAGTTPEGGTISGSSSIVQLDAWNWEDAAYKMDNAIHLTMPTFLPRPRR
                     FGGGGGRFAGLQAPTDPTKEALAKVEEIKAFFREAKAYNAETTHKETNLKFEAVKGLF
                     TKQQKLFVHGDQVKQMLIAIDFAKEFGFDVTIVGGSESWLIADLLKQNNISVLLQQLH
                     ALPTTEDDDVDQPFKTPAQLQKAGVLFALNDNHEESRYRNLNFNAGTAVAYGLTKEQA
                     LQAITLNAAKICGIDDRTGSLEAGKDANIVISGGDILDMRTSLIEHCFIQGREVSLEN
                     KQTQLYHRYMTKYGLQ"
     gene            1111..3546
                     /locus_tag="VKQ52_10820"
     CDS             1111..3546
                     /locus_tag="VKQ52_10820"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HLZ87728.1"
                     /translation="MKLLPALLFPAILLSSGLHAQPPAAAPREAREATRTQLPNGWSL
                     TPAGQSLPLSSDLPLNIALSPDGIHAAVTNNGNGVQSIDLINLKEQRVTASVPIGKAW
                     LGLAFARKQPLLYASGGNDDWIIRYTVKADSLINYDTLILGRPWPADKISPTGLALDE
                     GRDRLYVVTKEDKSLYIVDLKTKKPVLTVPLHSEAYTCLLNPLKAELYISAWGGRKVW
                     IYNTERDRLQDSVTTEDHPTDMALDRKGRTLFVANANANSVSVIDLPAHNVIETLHTA
                     LEPDAPTGSTTNSLTLDAAGKTLYIANADNNCLAVFDVAHPGHSVPKGFIPVGWYPTA
                     VRIAGRNLLVANGKGLSSMANPDGPSPMGHPANYKKTDATQQKVQYIGSLFKGTLSVI
                     PVPDAATLAKYTRQVYANTPYSQAVAQTASGEAGNPIPRRVGEGSPIKYVFYVLKENR
                     TYDQVLGDLPQGNGDTSLCLFGREVTPNAHAITEEFVLLDNFYVDAEVSADGHNWSMA
                     GYATDFVEKNWPTNYSGRGGEYDFDGSRPVANPTKGFIWDYCARAGVSFRNYGEFTDN
                     GYPTLAVLKQKEHYCKAYPGWDLGIQDIRREKVFEADFDSLLQADALPQFSTIYLPND
                     HTSGLYKGAYSPIAQVADNDLALGRLVEHISHSRIWGQSVILVLEDDAQDGPDHVDAH
                     RSPAYVISPYIKRHSVNHTPYSTTSVLRTMELILGLPPMSQFDAAATPLWSCFIPTPD
                     TTAFTAMPARVDLNTRNTASTPSASLSRHFDLSDADRIPDQLLNAVLWKAIKGEDSPV
                     PQPRRSAFVRVTP"
     gene            complement(3602..>5564)
                     /locus_tag="VKQ52_10825"
     CDS             complement(3602..>5564)
                     /locus_tag="VKQ52_10825"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HLZ87729.1"
                     /translation="AAIDAQRSYIESIRTYPINTEIRTVKTFIAATTPPVAPTIGGRA
                     PAGPLAAAEVSGAVTVELNNSFYLLPRTPMRRRPFDTRVGFFASHYTTYGDDQQRVEE
                     TEFIHRWRLEPKEEDIEKWKRGELVEPKKQIVYYIDPATPKKWRPYLIAGINDWQAAF
                     EKAGFRNAIVGKEWPENDRTMSLEDARFSVIRYFASDIENAYGPNVADPRSGEILESH
                     IGWYHNVMKLVHDWYMVQTAAVDPRARKMKFDDALMGDLIRFVSSHEVGHTLGLRHNM
                     GSSSKTPVEKLRDKAWVEANGHTASIMDYARFNYVAQPEDNITPRGLYPRIGDYDKWA
                     ILWGYKGIPDTKDEEEDKKILNHWVIDSLKNPRLWFGGEGQNFDPRAQTEDLSDNAMK
                     ASEYGIKNLKRILPNLPEWCREEADHYDNLEEMYQQVVGQFGRYMGHVVKNVGGVQET
                     FKSIEQQGDVYEATPKATQKEAVDFLNKNLYATPTWLLNKDILNKFSNPGLEDLLSTV
                     QANNLKSLLSPARLFRLYICTSRYGSSAYSVDELLADAQKGVWSELGPATPIDIYRRK
                     LQKEYITDLIALLNAEPPPPPPAGLPRGFNVFSGDIRNTDVPSSARAQLVELRTQIAG
                     AIPRETDKASKYHLQDVQERIKQALNPKQ"
BASE COUNT         1172 a         1840 c         1414 g         1138 t
ORIGIN      
        1 ctctccgtga ccgatgtagg tctgaaagag atcggcagcg gcgtccgggg ttccaacgac
       61 ttccaggaac tcggcgacct caaccccagc atccgctcca tcgtagccta taacacggat
      121 tcgaagatca tcggcaccct cagggccaac ggtatcctgc tggccggcac cacccccgaa
      181 ggcggcacta tcagcggctc atccagcatc gtccagctcg acgcatggaa ctgggaggac
      241 gccgcttata agatggataa cgccatccat ctcactatgc ctacctttct cccgcgcccc
      301 cggcgttttg gcggtggagg cggccgcttc gcaggcttgc aggcgcccac cgatccgaca
      361 aaggaagccc tcgccaaggt cgaagagata aaggccttct tccgtgaggc aaaggcctac
      421 aatgccgaga ccacgcacaa ggagaccaac ctgaaattcg aggccgtaaa agggctcttc
      481 acgaaacaac agaaactgtt cgtccatggc gaccaggtga aacagatgct catcgccatc
      541 gatttcgcaa aggaattcgg gttcgacgtg accatcgtcg gcggcagcga aagctggctt
      601 atcgccgacc tcctgaaaca gaacaatatc tccgtcctcc tccagcaact ccatgccctg
      661 cccaccaccg aggacgatga tgtcgaccag ccctttaaaa cgcccgcgca attgcaaaag
      721 gccggcgttc tctttgccct caatgataac cacgaagaga gccgttatcg caacctgaat
      781 ttcaacgccg gaacggctgt tgcctacggc ctcacaaagg agcaggcgtt gcaggccatc
      841 accctcaacg cggcaaagat ctgcggtatc gacgaccgca cgggctcact cgaggccggc
      901 aaggacgcca atatcgtcat cagtggcggc gatatcctcg acatgcggac aagcctcatc
      961 gaacactgct ttatccaggg ccgcgaggta agcctggaga acaagcagac gcaattgtat
     1021 catcggtata tgacgaagta cgggctacaa taatgattcc ataacgagcc ccgcgcaagc
     1081 ggggcttttt ttatgcctat ttttgagggc atgaaactcc tccccgcgct ccttttcccc
     1141 gccatcctcc tctcgtccgg cctgcacgcc caacctccgg cggctgcccc ccgggaagcc
     1201 cgggaagcta cccgcaccca actccccaac ggctggtccc tcacccccgc cggccaatcc
     1261 ctcccgctaa gctccgacct gcccctcaat atcgccctgt ctcccgatgg catccatgcc
     1321 gccgtgacaa acaatggcaa tggcgttcaa tccatcgatc ttatcaacct gaaagaacag
     1381 cgggtcaccg cctccgtccc catcggaaag gcctggctgg gcctggcctt tgcccgaaaa
     1441 caacctttac tctatgcctc cggcggtaat gacgactgga tcatccgcta caccgtcaaa
     1501 gccgacagcc tcatcaacta cgataccctc atcctgggcc gtccctggcc ggcggataag
     1561 atcagtccca ccggtctcgc cctggatgaa ggccgcgaca ggctgtatgt cgttacgaag
     1621 gaagacaagt cgctctacat cgtcgatctc aaaacaaaaa agccggtcct gacggtaccc
     1681 ctccattccg aagcctatac ctgcctgcta aatccactca aggccgaact ctatatctcc
     1741 gcctggggag gccgtaaggt ctggatctac aacaccgaaa gggacagact gcaggattcc
     1801 gtgacgacgg aagatcatcc caccgacatg gccctcgacc gcaagggccg caccctcttc
     1861 gttgccaatg ccaatgccaa ttcggtgtcc gtcatcgatc tccctgcgca taacgtcatc
     1921 gaaaccctcc acaccgccct ggagcccgac gcccccacag ggtccaccac caacagcctc
     1981 accctcgatg ccgcgggcaa gaccctctat atcgccaatg ccgacaacaa ctgcctcgcc
     2041 gttttcgatg tcgcccatcc gggacatagc gtcccgaaag gatttatccc cgtcggctgg
     2101 tatccgacgg ccgtgcgcat cgccggccgc aacctgctcg tcgccaacgg caagggcctt
     2161 tcttcgatgg cgaacccgga tggtcccagt cccatggggc acccggcaaa ctataagaag
     2221 acggatgcca cgcaacagaa agtacaatac atcgggagcc tgttcaaggg tacgctatcg
     2281 gtgatccctg tccccgatgc cgccacgctc gcaaagtata cccggcaggt gtatgccaat
     2341 acgccttatt cccaggccgt cgcgcagacg gcatccggcg aagcgggcaa ccccatcccc
     2401 cgcagggtgg gagagggttc ccccatcaaa tacgtgttct atgtgttgaa ggagaaccgc
     2461 acctacgacc aggtactggg tgatctcccc cagggcaatg gcgacaccag cctttgtctc
     2521 tttggcagag aggtcacccc caacgcccac gcgatcaccg aagaattcgt gctgctggat
     2581 aatttctatg tcgatgcgga ggtcagcgcc gacggccata actggtcgat ggccggctat
     2641 gccacggatt tcgtggaaaa gaactggccc accaactata gcggtcgcgg cggggaatat
     2701 gattttgacg gaagccgccc cgtggccaat ccgacaaaag ggtttatctg ggactactgc
     2761 gcccgcgccg gggtatcctt ccgtaattat ggggaattca cggacaacgg ttatcccacc
     2821 ctcgccgtgc tgaaacaaaa ggaacattac tgcaaggcct acccgggctg ggatctgggc
     2881 atccaggata tccgccggga aaaggtcttc gaagccgatt tcgattccct gctgcaggcg
     2941 gatgccctgc cgcagttcag cacgatctac ctgccgaatg accataccag cggtctctac
     3001 aagggcgcct acagccccat cgctcaggtg gcggacaatg accttgccct gggaagactg
     3061 gtggaacata tctcccatag ccggatctgg ggacagtcgg tgatcctcgt actggaagac
     3121 gatgcccagg acggccccga ccatgtcgac gcccaccggt cgcccgccta tgtcatcagc
     3181 ccgtatatca aacggcattc cgtcaaccat accccttatt ccactacctc ggtccttcgt
     3241 accatggagc tgatcctcgg tctgccgccc atgagccagt tcgatgcggc agccacgccg
     3301 ttgtggagtt gttttatccc tacaccggac accaccgcct tcacggcgat gcctgcgcgg
     3361 gtcgatctca atacccgcaa tacggcctcg acgccttcgg cctccctgtc ccggcatttc
     3421 gatctctccg atgccgaccg gataccggac cagctgctca acgccgtcct ctggaaggcg
     3481 ataaagggcg aggacagccc cgttccccag ccccgccgca gcgcctttgt tcgcgtaacc
     3541 ccataaaaaa aggagaccgc ctcaagtcaa acctgggcgg cctcctttat acgaacgtcg
     3601 tttactgttt cgggttcaat gcctgtttta tcctctcctg cacatcctgt aaatggtact
     3661 tactcgcctt atccgtctcc cgggggattg ctcccgcgat ctgtgtcctc agctccacca
     3721 gctgcgcccg ggcgctggag ggcacatccg tgttccggat gtcaccggag aacacattga
     3781 accccctcgg cagccccgcc ggcggcggtg gcggcggctc ggcattcagc agcgcgataa
     3841 gatcggtgat atattccttc tgcagcttcc gccggtaaat atcgatcggc gtggcaggcc
     3901 ccagttccga ccagacaccc ttctgtgcat cggcaagcaa ctcatcgacg ctataggcgc
     3961 tgctgccata tctcgacgta cagatgtata gccggaacag ccgtgccggc gacagcaggc
     4021 tcttcagatt attggcctgt accgtactca acagatcctc cagaccggga ttactgaact
     4081 tattcaggat atccttattc aacaaccacg tgggtgtggc ataaagattc ttattgagaa
     4141 aatcgacggc ctccttctgt gtggccttcg gcgtggcttc atatacatct ccctgctgct
     4201 cgatgctctt aaaggtctcc tgtactccgc cgacattctt caccacatgc cccatataac
     4261 ggccaaactg cccgaccacc tgctggtaca tttcttcgag gttgtcgtag tggtctgcct
     4321 cctccctgca ccactcgggc agattgggca ggatacgctt caggttcttt atgccgtatt
     4381 cgcttgcctt catggcattg tcgctgagat cctcggtctg cgcacgcgga tcgaagttct
     4441 ggccttctcc gccaaaccag agccgcgggt tcttcaggct gtcgatcacc caatggttga
     4501 ggatcttctt gtcctcctcc tcatccttcg tgtcggggat gcccttatac ccccagagga
     4561 tcgcccattt atcatagtcg ccgatccggg gatacagacc cctgggggtg atattgtcct
     4621 ccggctgcgc cacatagttg aaccgggcat agtccatgat ggaagcggtg tgcccattgg
     4681 cttccaccca ggccttgtcc cgcagcttct ccaccggcgt cttgctgctg ctgcccatat
     4741 tgtgacgcaa cccaagcgta tgccccacct cgtgagacga tacaaaccgg ataagatcac
     4801 ccatcagtgc atcgtcgaac ttcatcttcc gcgcccgcgg atccaccgcc gccgtctgga
     4861 ccatatacca gtcatgcacc agcttcatta cattatggta ccagccgata tgactctcca
     4921 ggatctcccc gctgcgcgga tcggcgacgt tcggtccata ggcgttctcg atatcgctcg
     4981 caaaataccg gatcaccgag aaacgcgcat cctcgagcga catcgtccgg tcgttctccg
     5041 gccattcctt gccgacaatg gcattcctga agccggcttt ctcaaaagcg gcctgccagt
     5101 cgttgatccc ggctatcaga taaggccgcc attttttagg cgtggccgga tcgatataat
     5161 acacgatctg tttcttcggt tcgaccagct ccccgcgctt ccatttctcg atgtcctcct
     5221 cctttggctc gagccgccag cgatggatga attccgtttc ttccacccgt tgctggtcat
     5281 ccccataggt ggtgtaatga ctggcgaaaa aaccgacccg cgtatcgaag ggtcgccggc
     5341 gcatgggcgt cctgggcagc agataaaaag agttgttcag ttccaccgtc accgcgcccg
     5401 atacttcggc cgccgcaaga gggccggcag gcgcccgtcc gccgatggta ggcgccacgg
     5461 gtggcgtggt tgcggcgata aaggtcttca ccgtccggat ctccgtattg atggggtagg
     5521 tgcggatgct ctcgatatag gaacgctggg catctatcgc cgcc
//
LOCUS       DATGFG010000578         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_800736, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000578 DATGFG010000000
VERSION     DATGFG010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800736"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VKQ52_10830"
     CDS             complement(<1..619)
                     /locus_tag="VKQ52_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87730.1"
                     /translation="MDMYLLLRNNKQSGPYSFEELKGMGLKAYDLVWLEGKSAAWRYP
                     CEIDELSAFAPQVEEQPFDRFYKRPTATASASATATATAPVKSPAGSTTLSTTTAPPV
                     TTTTTSAPPAAPAATPAPAAPLYTGEPSAVPGKRIIYVTMPAGRTPAPVREIPREPVR
                     ELPKEPARPAQALAPVAQPGIPDPAHRAYASGPGYTSPRAEDYSSQ"
     gene            913..>2353
                     /locus_tag="VKQ52_10835"
     CDS             913..>2353
                     /locus_tag="VKQ52_10835"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HLZ87731.1"
                     /translation="MDAAIQEKVNAWLTGNYDQATKDEIARLQKENPTELTESFYRSL
                     EFGTGGLRGIMGVGTNRMNKYTVGMATQGYANYLKASFPGKEVRVAIAHDSRNNSRFF
                     AETAANVFAANGIKVFLFQSLRPTPELSFAIRHLHCQGGLVCTASHNPKEYNGYKAYW
                     DDGGQLVPPHDANVIKEVEKIAGVEAVKWGGGESNISMIGKDLDEAYIAMVRNLSVYP
                     EVIERQHNLRIVYTPIHGTGIKLVPEVLQRFGFTNVHVVDEQATPDGNFPTVIYPNPE
                     ESEAMSIGLRKAQQIDADILLGTDPDADRVGIGVKDRKGKWVLMNGNQTAVLAFNYMI
                     EARKAKGIAGATDMVVKTIVTTNLIDEIAARNGVECYNVLTGFKYIAELIKEKEGKKN
                     YVIGGEESYGLMIGSQIRDKDAVSAVALLCEMAAYEKDKGRSLYEKLIDLYVEYGFYK
                     EHLISITKKGMNGQKEIAEMMSAYRSHPPA"
BASE COUNT          561 a          554 c          748 g          490 t
ORIGIN      
        1 actgtgaaga ataatcctca gcgcgggggc tggtataacc gggaccggag gcgtaggcgc
       61 ggtgtgccgg atcggggatg ccgggctgtg caaccggggc cagggcctgt gcaggccggg
      121 ccggttcttt tggcagttcg cgaaccggtt cgcggggtat ctcgcgaacg ggtgccgggg
      181 tccggccggc gggcatcgtc acatagatga tccgttttcc gggaaccgcg gagggttcgc
      241 cggtatagag gggggccgcc ggagcaggcg ttgctgcggg tgcggccggg ggggccgatg
      301 tggtggttgt cgtaaccgga ggggccgtcg tggtggagag ggtagtggat ccggcgggcg
      361 atttcaccgg ggccgtggca gtcgcggtag ctgatgcgct agcggtggcc gttggccttt
      421 tgtagaaacg atcgaagggc tgttcttcca cctggggagc aaatgcgctg agctcatcta
      481 tctcacaagg atatctccac gccgcacttt tcccttccaa ccataccagg tcgtaggctt
      541 tcagccccat ccccttcaat tcttcgaaag agtatgggcc gctttgcttg ttattacgaa
      601 ggagcaaata catatccatg ttccggttaa gaggcgttta cgatttcagg cggtacagag
      661 aataacagca aatttttcac ctgatattgt cccgctaatg tgcttaacat aaatttagcg
      721 aagagtaagt tatgacttac caaaccgggc tttcaatgga tttgaggagg aagaacaggg
      781 ccggagtcgt tggcccaata caaatgtaca attttgatca ataataacat agcgtcgcgg
      841 aatctttctg ccgggatttg ttattgttcg ttttcttgcc gatcttcgga acccaatata
      901 aattaatgca atatggatgc tgctatacag gaaaaagtga atgcatggtt aacgggaaac
      961 tatgaccagg ccacgaagga cgagatcgcc cggctgcaga aagagaatcc cacggaattg
     1021 acggaaagtt tttaccggag cctggaattc gggacggggg gattgagggg catcatgggg
     1081 gtggggacca accggatgaa caaatacacc gtgggaatgg ccacgcaggg atatgccaac
     1141 tatctgaagg cttcgttccc gggtaaggaa gtacgggtag ccatcgcgca tgacagccgc
     1201 aacaacagcc gtttttttgc ggaaaccgcc gctaatgtat tcgcagccaa tgggataaaa
     1261 gtatttctct tccagtcgct gcggccgacg cccgaactga gctttgccat tcgtcacctg
     1321 cactgccagg gtgggttggt gtgcacggct tcgcataacc caaaggaata caacgggtat
     1381 aaggcttatt gggacgacgg cggccagctg gtgccgcccc acgacgcgaa tgtcataaaa
     1441 gaagtggaga agatcgcggg ggtggaggca gtgaagtggg gcgggggtga atcgaatatc
     1501 agcatgatcg ggaaggacct ggatgaggcg tatatcgcca tggtgcggaa cctttctgtt
     1561 tacccggaag taattgagcg tcagcataat ttgcgaattg tatacacccc catccacggt
     1621 acggggatca agttggtgcc cgaggttctc cagcggttcg ggtttaccaa tgtacatgtg
     1681 gtggatgagc aggccacccc cgatggcaat tttccgaccg tgatctatcc gaaccccgag
     1741 gaaagcgagg cgatgagcat cggtctacgg aaggcccagc agatcgacgc cgatatcctt
     1801 ctgggtaccg atccggatgc cgaccgggtt ggcatcgggg tgaaggacag gaaggggaag
     1861 tgggtgctga tgaatggtaa ccagacggcc gtactggcct tcaactatat gatcgaggcc
     1921 cgtaaggcga aggggatcgc cggcgccacc gatatggtgg tgaagacgat cgtgacgacc
     1981 aacctgatcg acgagatcgc cgcgcgcaac ggcgttgaat gttataatgt ccttaccggg
     2041 ttcaaataca tcgccgagct catcaaggaa aaggaaggaa agaagaacta tgtcatcggc
     2101 ggggaggaaa gttacggcct gatgatcggc agccagatca gggacaagga tgcggttagc
     2161 gccgtggccc tgctttgcga aatggcggcc tatgaaaaag acaagggccg cagcctgtat
     2221 gagaagctga tcgacctgta tgtcgaatac gggttctata aggaacatct catctccatc
     2281 actaagaagg ggatgaatgg gcagaaggag atagcggaga tgatgagcgc ctaccgcagc
     2341 catccacccg ccg
//
LOCUS       DATGFG010000579         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_37789, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000579 DATGFG010000000
VERSION     DATGFG010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37789"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="VKQ52_10840"
     CDS             <1..1376
                     /locus_tag="VKQ52_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87732.1"
                     /translation="EMDGKGDEIFVNCNWIMAGRNNATEFEGKYRSPMYGDVNRHYGW
                     VQAGSRSDKGGIRSGDQYRGYSAIGEFDISDGETLTIMPTIWEWDGNEDEMTAFMSFL
                     TGHVNEIKNASLQLIQGVNQLSGGNTSQFLLDMGNTNLSLFSMAVNAILGQAKDRPIG
                     LDAAGRFKSKAFVLNRGLCRDIAQRNLGYGLGVLQMDYDENQLGNTRDHGAYTILIKI
                     ELLGSGSTTATTPSNPPGRYIPPPMTTPPAGTATHGSAPVSNPPAGTPTHGSTPVSNP
                     AGGSSYIPPPSTNSPGAASHGSAPVSTPASGGRYIPPPVANPAGRGGAPAGAPNGASP
                     AGPASPSANPAGPSANPAGPAGGAVSIPGTWTGTYGDGPSSTSHGFFSLQFNADGSLL
                     MLDPRRAVLARGSYVMNGISMTGSLSWLAGGRYLIAGRTKGPTMTGTWGRETSNFNGG
                     NWTLTKQ"
     gene            1384..2814
                     /locus_tag="VKQ52_10845"
     CDS             1384..2814
                     /locus_tag="VKQ52_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87733.1"
                     /translation="MHNSLKQLLAVSSLFLGLGSGALGQKASFDLASFLPPAGWQVND
                     LGGAVSFTASDSAKGTLCILTIYKSADGSGNADQDFKQDWNTKMIGGFGTSASPRIKK
                     TPAKNGWESRIGVDTFRRNGIVYLGILAVMSGYNKTMDMLVITNRKEYLNTLDPFYSS
                     IEMQRPAATGPANAMAGAGTAATGSAVAGVSVAHGDMDAIRYTLPRGWTEGRTAEGLV
                     TASSPLLECTGESYYTLYVNGVMDYDGDLQELGLKIHKSCFYQQDPVFQYPEAGRRVV
                     KGIDDQGREFLSYEAPAFIFKSDNSWHYGYIYLLRSGGKVATFMLELRPQNRDRFASP
                     SAMTLYFLYPCNAIRSIWNKFAASVSFVNPGPKAEYDPADLTGRWSCHITLGASIWGY
                     VSDLPLQKYHFADDGRWQSEKMITQNNFGRYSVSGDKLLITDGAGKTISYRFKLERVF
                     EYNNWSRHITLYDSNGTETELVYDGD"
BASE COUNT          670 a          924 c          811 g          521 t
ORIGIN      
        1 tcgaaatgga cggtaagggt gatgagatct tcgtcaattg caattggatc atggccggca
       61 ggaacaatgc gacggaattc gaaggcaaat accggtcgcc catgtatggc gacgtgaacc
      121 gccactatgg ttgggtgcag gcgggcagcc gcagcgacaa gggcggtatc cggtcgggcg
      181 atcaataccg gggttattcg gcgatcggcg aattcgatat cagtgacggc gagacattga
      241 cgatcatgcc gaccatctgg gaatgggacg gcaacgagga tgaaatgaca gcgttcatga
      301 gtttcctgac agggcatgtc aacgagatca agaacgcaag tcttcagctg atccagggcg
      361 tcaaccagct ttccggcggc aacacaagcc agttcctcct ggacatgggg aatacgaatc
      421 tgtcgttatt ctccatggct gtgaacgcca tcctcggaca ggcaaaggat cggccaatcg
      481 gactcgatgc cgccggccgc ttcaaatcga aggccttcgt cctcaaccgg ggcttgtgcc
      541 gggacatcgc gcaacgcaac ctgggatacg gactgggcgt gctccaaatg gattacgacg
      601 agaatcaact gggcaacacc cgcgatcatg gagcctatac gatcctgata aagatcgaat
      661 tgctcggttc gggttcaacc accgccacta ctccttcgaa ccctcctggc cgttatattc
      721 cgcccccaat gactactcct cccgccggca cggctaccca cggtagcgca ccggtatcaa
      781 atccccccgc cggcacacct acccacggta gcacacccgt atccaacccc gccggaggca
      841 gcagctatat tcccccaccc tcgacaaact cccccggtgc agccagccac ggcagcgcgc
      901 ccgtatccac ccccgcctcc gggggccggt atatcccacc tcctgttgcc aatcccgccg
      961 gccgcggcgg agcacccgcc ggcgccccga acggcgcttc accagccggg ccagccagcc
     1021 catccgcaaa ccctgcggga ccatccgcaa accccgcggg cccggccggt ggcgccgttt
     1081 cgatccccgg aacctggacc ggaacctatg gcgatggtcc cagcagcacc agccatggct
     1141 tcttcagcct ccagttcaac gccgacggaa gcctgctcat gctggacccg cgccgggcgg
     1201 tcctggcgag aggctcctat gtgatgaatg gcatctcgat gaccggctct ctttcctggt
     1261 tggcgggcgg acgatatctc atcgccggcc ggaccaaggg cccgacgatg accggcacct
     1321 ggggccgcga gaccagcaat ttcaatggtg gaaactggac cctgacaaaa caataatcag
     1381 cgtatgcaca actcacttaa acaactgctg gcggtcagca gcctcttcct gggactggga
     1441 tccggcgccc tcggccagaa agccagcttc gatctcgcca gcttcctgcc acccgccggt
     1501 tggcaggtga acgatctggg tggcgcggtc tctttcaccg cctccgactc ggcgaagggc
     1561 accctctgca tcctcacgat ttacaagagc gccgacggca gcggcaacgc ggaccaggat
     1621 ttcaaacagg actggaacac aaagatgatc ggcggtttcg ggacctccgc cagtccccgg
     1681 atcaaaaaga cgccggcaaa gaacggatgg gaatcccgga tcggggtcga taccttcagg
     1741 aggaacggga tcgtttacct cggaattctc gccgtaatga gcggctacaa caagaccatg
     1801 gacatgctgg tcattacaaa ccgcaaagaa tatctcaata cgttggaccc cttctattcc
     1861 agcatcgaga tgcaacgacc tgcggcgacg ggtccggcca acgcaatggc cggcgccggt
     1921 acagcagcga cgggatctgc tgttgccggc gtttccgttg cccacggcga catggatgcc
     1981 atccgctata ccctgccaag gggatggacc gaaggcagga ccgccgaggg actcgtcacc
     2041 gcctcctccc cgctgttgga atgcaccggc gaaagctact acacgctcta tgtcaacggg
     2101 gtgatggatt acgacggcga cctgcaggaa ctgggcctga agatccacaa aagctgcttc
     2161 taccagcagg acccggtgtt ccaatacccc gaggcaggtc ggcgggtggt aaaaggcatc
     2221 gacgaccagg gacgggaatt tctttcctat gaggcgccgg cctttatctt caagtcggac
     2281 aacagctggc attacggcta tatctatctg ctgcggagcg gtggaaaggt ggccaccttc
     2341 atgctggaac tgcggccgca gaaccgcgat cggtttgcct cgcccagcgc catgaccctg
     2401 tatttcctgt acccctgcaa cgccatccgg agcatctgga acaaattcgc cgcatcggtg
     2461 agctttgtca acccgggtcc gaaggcggaa tatgatccgg ccgatctgac gggcagatgg
     2521 tcatgccata tcacgctggg cgcctcgatc tggggctatg tcagcgatct ccccctccag
     2581 aaatatcatt tcgcggacga tggccgctgg caatccgaaa agatgatcac ccagaacaat
     2641 tttggccggt acagcgtcag cggggacaaa ctcctcatca ccgatggggc ggggaagacc
     2701 atcagctacc gcttcaaact ggaacgcgta ttcgaataca ataactggag tcgccacatc
     2761 acgctttacg acagcaacgg aacggaaacc gaactggtct atgatggcga ctaaataccc
     2821 cattaaaaat aaagtaacat gagaaagcta cccctgctcc tggtcttcat ctccctggcg
     2881 tccgccgtca ccgcacaaca gccttcgcaa aaagaaatgg aaaaag
//
LOCUS       DATGFG010000580         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_875515, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000580 DATGFG010000000
VERSION     DATGFG010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875515"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VKQ52_10850"
     CDS             complement(<1..920)
                     /locus_tag="VKQ52_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HLZ87734.1"
                     /translation="MPTAAYIVLIPLLPLAGFAILGLFGRKYLGTFSGILGTGLVLGS
                     TVLSQWVAYAYFFIDVLPGGVHVPITAFKATWLSFTPALSIDMSIILDPISVMMIVVV
                     SFISLMVHIFSLGYMKGEERYGTYYAFLGLFTFSMLGLVLSGNIFETYIFWELVGCSS
                     FLLIGYYYDRPTAVAAAKKAFIVTRFADLGFLIGILILSFQAHALDFNGLIRNLAAQT
                     RPGIANPASFLGFPLVSWGLILVFIGGAGKSALFPLHIWLPDAMEGPTPVSALIHAAT
                     MVVAGVYLVARLYLLYALAVPLPQILLYVGA"
     gene            complement(931..1245)
                     /gene="nuoK"
                     /locus_tag="VKQ52_10855"
     CDS             complement(931..1245)
                     /gene="nuoK"
                     /locus_tag="VKQ52_10855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HLZ87735.1"
                     /translation="MTQIPLTHILFVSTALFFIGMYGLFTRRNMITMLMSVELILNSV
                     NINFVAFNRYLYPEKLEGVFFSLFIVAIAAAEAAVAIAIIINLYRNLHTIDVEEADEM
                     KF"
     gene            complement(1242..1760)
                     /locus_tag="VKQ52_10860"
     CDS             complement(1242..1760)
                     /locus_tag="VKQ52_10860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HLZ87736.1"
                     /translation="MSASAVVFYILSTFILVAGLLAVTSRKIFRSAIWLLFSLTGIAG
                     LYFWLQMEFIAAVQIIVYVGGIVVLIIFSIFLTQQSGKEMPPPFRSRAIFSALAVCTG
                     FALTCILLRNYTFPAGGHAAPDTGVANIGSQLLSTTEHGYVLPFEVISILLLAAMIAC
                     IVIALKTKPGDK"
     gene            complement(1760..2239)
                     /locus_tag="VKQ52_10865"
     CDS             complement(1760..2239)
                     /locus_tag="VKQ52_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HLZ87737.1"
                     /translation="MAFLKDYISDIYRGARSLLKGMKLTGYYFTHRKEIITEQYPDNR
                     ADMKLPERFRGEVVMLHDENNEHACTGCTACELACPNATIKIITKFDTTPDGKKKKAL
                     DTFVYHLELCTMCNLCIQACPTDAIKMAQNFEHSVYDRSQLTKKLNKPGSKIREGVE"
     gene            complement(2227..>2897)
                     /locus_tag="VKQ52_10870"
     CDS             complement(2227..>2897)
                     /locus_tag="VKQ52_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HLZ87738.1"
                     /translation="ISSLSVIGILMAGWASNNKYSLLGAMRSGAQIVSYELSAGLSVI
                     SIITLTGSLRISDIIASQQDGWWLFKGHIPAIIAFIIFIIAVTAETNRAPFDLAEAES
                     ELTAGFHTEYSGMRFALFFLAEYVNIFIVCSIAATLFLGGWMPLHIGHWTGFNHVMDF
                     IPSSLWFIGKTFFLIFVIMWFRWTFPRLRIDQLLDLEWKYLLPISLFNLLLTTLIAIE
                     GWHF"
BASE COUNT          721 a          675 c          921 g          580 t
ORIGIN      
        1 gcgcctacgt aaagcaggat ctgcggcagc gggaccgcga gggcatagag caggtacagg
       61 cgggctacga ggtagacgcc cgccacgacc atggtcgcgg catggatgag ggcggatacc
      121 ggagtgggcc cttccatggc atccggcagc cagatgtgaa ggggaaagag ggcggattta
      181 cccgcgccgc cgatgaatac gaggatcagg ccccaggaga ccagggggaa gccgaggaag
      241 gatgcggggt ttgctatgcc cggccgggtc tgggcggcaa ggttccggat caggccgttg
      301 aaatccaggg catgcgcctg gaaggaaagg atcaggatac cgatgaggaa gccgagatcg
      361 gcgaagcggg taacgataaa ggcttttttt gccgcggcga cggccgtcgg gcgatcatag
      421 taatagccga tgagcaggaa ggaggagcag ccgacgagtt cccagaagat ataggtttcg
      481 aagatattgc cggatagcac caatcccagc atcgagaagg tgaagaggcc gagaaaggca
      541 taataggttc cgtagcgttc ctcgcctttc atgtagccga ggctgaagat atgcaccatc
      601 agggagataa aggaaaccac gacgatcatc atcaccgaga tagggtccag gatgatgctc
      661 atgtcgatgg aaagggccgg tgtaaaggat agccaggtgg ccttgaaggc cgtaatgggg
      721 acatggacac cgccgggcag tacatcgata aagaaatagg cataggcgac ccattgggac
      781 aggaccgtgc tacccaacac gaggccggtg ccgagaatgc cggaaaaagt ccccaggtat
      841 tttctgccga acagccccag gatggcaaag ccggccagcg gcaggagggg gatcaggacg
      901 atataagcag cagttggcat agttacaggt tcagaatttc atttcatccg cttcctccac
      961 atcgatggtg tgcaggttcc ggtatagatt gatgatgatg gcaatggcca cggcggcctc
     1021 ggcggcggca atggcgacga tgaacaggga aaaaaagact ccttccagtt tttccgggta
     1081 caggtatctg ttaaaggcga caaaattgat gttgacgctg ttgaggatca gttccaccga
     1141 catcagcatc gtgatcatat tccggcgggt gaacaggccg tacatcccga tgaagaagag
     1201 ggcggtgctg acaaacagga tatgggtcaa cgggatctgg gtcatttgtc tccgggtttt
     1261 gtttttaaag cgatgacgat gcaggcgatc atggccgcca gcagcaggat gctgatcact
     1321 tcgaagggca ggacataccc gtgttcggtg gtgctgagca actggctccc gatgttggcg
     1381 acaccggtgt cgggcgcggc atggccaccc gcggggaagg tataattacg caaaaggata
     1441 caagtgaggg caaagcccgt acaaaccgcc agtgcggaga aaatggcgcg gctccggaag
     1501 ggtggcggca tttcctttcc cgattgctgg gtgaggaaga tggagaagat gatcagtacg
     1561 acgatgccgc ctacatagac gatgatctgt actgccgcga tgaattccat ctgcagccag
     1621 aaatagaggc cggcaatacc cgtgagggag aacagcagcc agatagctga gcggaaaatt
     1681 ttcctggagg tgaccgcgag caggccggct accaggataa aagtggaaag tatgtaaaaa
     1741 acgacggcag acgcgctcat tattcaacgc cctctctgat tttagaaccc ggtttattta
     1801 atttttttgt cagttggctg cggtcatata cactatgttc aaaattctgg gccattttta
     1861 tggcatccgt ggggcaggcc tggatgcaga gattgcacat cgtacagagc tccagatgat
     1921 agacaaaggt atcgagcgcc tttttcttct tgccatcggg ggtcgtgtcg aatttggtga
     1981 tgatctttat cgttgcgttg ggacaggcga gttcacaggc ggtacagccg gtgcaggcgt
     2041 gttcgttgtt ctcatcgtgc agcatgacca cctccccgcg aaacctttcg ggcagtttca
     2101 tgtcggcccg gttgtcgggg tattgctcgg tgatgatctc cttccggtga gtaaaatagt
     2161 agcccgtcag cttcatccct ttcaggaggg agcgggcgcc gcggtagatg tccgatatat
     2221 agtcttttaa aaatgccatc cttctattgc tatgagcgtc gttaataata ggttgaacag
     2281 gcttatcggc aggagatatt tccattcgag atccagcagc tggtcgatgc ggaggcgcgg
     2341 gaaggtccag cggaaccaca tgatcacgaa gatgaggaag aaggtcttgc cgataaacca
     2401 gagggaggac gggataaaat ccattacgtg attgaacccg gtccagtgac cgatgtgcag
     2461 cggcatccac ccccccagga agagggtggc ggctatcgag cacacgataa agatattgac
     2521 atattcggcc aggaagaaca gggcgaatct catcccggaa tattcggtgt ggaagccggc
     2581 ggtgagctcc gattccgctt cggccagatc gaagggggct cggttggtct ccgccgtgac
     2641 ggcgatgatg aagatgataa aggcgatgat ggcagggata tgtcccttga acagccacca
     2701 gccgtcctgc tgtgaggcga tgatatcgga gatgcggagg ctgcccgtca gggttatgat
     2761 ggagatgacg gagagtccgg cggataattc atagctgacg atctgtgcgc cgctgcgcat
     2821 ggcgcccagc agggaatatt tattattgct ggcccagccc gccatcaaaa tgccgatcac
     2881 ggaaagggaa gagatag
//
LOCUS       DATGFG010000581         5565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1046484, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000581 DATGFG010000000
VERSION     DATGFG010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046484"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VKQ52_10875"
     CDS             complement(<1..1118)
                     /locus_tag="VKQ52_10875"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87739.1"
                     /translation="MRNKFIEMMCMGLVLFCSCQKSFLELSNPNAVTIPQFLTSETDV
                     LLALNGCYSAMKNNNAMGEESDLFTDQRSDDTGTNDNQSNAGEPFQFNNFSLLPTNSY
                     LYSHWKQMYNAIAQCNTLLGGIDKVTFTDSLKPTYTAEAKFLRAMLLFDLVREWGDIP
                     MSTVPLNTPAQITAATYRVPAVQVYNQIVADLTDATKSNLPVRQPAGNRGHASMQAVY
                     YLLGKVYLTKFATLDGGNSGAASNSDLDSANYCLTTCYNLRTFTNLGSIAYTDNFDVT
                     RKASCPELIFQLVYLQGDLNYYSHLATDAQAAGEYIDTKKTQSGVGYNVTHDLVKEYE
                     VTDPRDSFSIRYDSFKTVKDWYVTKFRDTSAAAGVNGYG"
     gene            complement(1129..4689)
                     /locus_tag="VKQ52_10880"
     CDS             complement(1129..4689)
                     /locus_tag="VKQ52_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ87740.1"
                     /translation="MTRKLQSSFMGCGLLFLFLLLGQRGWAQTIRLDLRGSDLSKVVA
                     ALQQQAPTVNFTYSQEVLEKVKVDRLQLKAQRLEDALEILQKRYGLHYLLDGSNLTLK
                     YVPLNAATDAPMAGSRRIEGLVADENGQPVRSATVHVKGTQNAVVTDDNGHYSIMVMA
                     GEHLEVSSVGYGTQDVLVKGVSPLDIRMHQGRDALNEVVFVGYQKMRKSDFTGAVASV
                     KASELNVSAPTAGQALVGKVAGVVVSQTDGSPYASPKIRVRGVGSFNASTDPLYVIDG
                     YPAGNDLYINPNDIESIDILKDAASAAIYGSRAAGGVVLITTKRGRPGKGKLEYDYQY
                     GVDQLAHKVKLLNGDQFAQLVIDGRNDSYKDLVQNQGLVWNDNMYLDNNATRVARVGN
                     GASISIPTELYDFTTGKEIPQKYNTDWQNELYRNAPFMRHNLAFSGGTQNMRYMISGD
                     YQSQDGIMLNTAQQRVNFRANVDGDVNDKLKVGASISYTGNTNHETQEGRWDHSPAFG
                     ALIYMPVFPAYNPDGSIAQYLADAESAQYGYQSIENPIATAELTQIKRQGNRATYNGF
                     ATYELIPHLLFKTNLGLQTYGEKYDYYLPTSLSSGTNPPYSAASIAAANAVAQQLNQK
                     DELAEFTLNYEKHFGPHAINILGGYTVQSTSSDLLSITAKGFQNDLIPQVTAGTPGQI
                     TINSGTGKSSYTLNSYLGRAQYNYEGKYYLTGSIRTDGSSRFGLHNKWAVFPSVSGGW
                     NISKENFYKGWLEEHTTLRLRASWGLSGNNNIGNYNTVQTYSSTGVVFGNNVISQAYI
                     TGNVQDPNLEWESTSQYNLGLDLGMFHDRIQLIANFYDSRSFNLLVNNNISAVSGFTS
                     ILTNLRNSRIRNQGFDIQVDGKVIQSSGGFNFNVSGNISINRNKVLNLGGASTIISDG
                     AERQYITHITETGQPIGMFYGYKVAGMVRASDTANINADTKVYSANGQTFPKGYVLKG
                     PPRSLAQTNPLRPGDLYFKDVNGDGVVNANDLSIIGSPYPKFTYGFSLSASYKAIDAS
                     ASFAGSYGNQVLDGQDYYLFNMEGSGNQYEVVSQRYRSESQPGNGHVYRASRAGTQSN
                     STRLSTFYLQDGSFLRCTNISIGYNLPDISVLHRAGLSAIRVYAGVNNVFTITKYKGY
                     NPEVDYNYSVGSSTANLTPGVDYGVYPLVRAYNMGVHVTF"
     gene            complement(4713..>5565)
                     /locus_tag="VKQ52_10885"
     CDS             complement(4713..>5565)
                     /locus_tag="VKQ52_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ87741.1"
                     /translation="RLLSEVEEQRLVQELWDRIAARENPEIGKDPENRLRHRRMLRYA
                     AVWAGMIMFSGGIWMQWNRRGGRSIARKASTYTTIGTAYQQVRKVLLPDSSIVWLNSA
                     THVSFPADFATHREVRLSGEAFFEVASDVHHPFVVIAGNTATHVFGTSFNISAYPEAG
                     ELRISLKSGKIGVEYGNKTQKVLLPGQLMVYDKESGNGEVLQQAPKEMDVWTAGRMLF
                     YKTPLKEALAQIEARYGVHIIYDHAITDRTITARFDNTALEKVLQSLSFGWDLHFVRN
                     GDTLHVR"
BASE COUNT         1236 a         1638 c         1410 g         1281 t
ORIGIN      
        1 ccatagccat tgacacccgc cgccgcactc gtgtcacgga atttggtcac ataccagtcc
       61 ttcaccgtct tgaaagaatc ataccggatg gaaaagctgt cccgcggatc agtcacctca
      121 tattccttca cgaggtcgtg cgtcacattg tatcccaccc cgctctgggt cttcttggta
      181 tcgatatatt caccggcagc ctgggcatcg gtagccaggt ggctataata attgaggtca
      241 ccctgcaggt acaccagttg gaagatcagt tcgggacagg aggccttcct ggtcacgtcg
      301 aaattgtccg tgtaggcaat gctgcccagg ttcgtgaagg tccgcagatt gtaacaggtg
      361 gtcaggcagt aattggcgct gtccagatca ctgttggacg cagccccgct gttaccccca
      421 tccagcgtgg caaacttggt caggtatacc ttccccagca aataatagac ggcctgcatc
      481 gacgcatgac cccggttgcc cgcgggctgg cgcaccggaa ggttgctctt cgtagcgtcg
      541 gtgaggtccg ccacgatctg gttatacact tgcaccgccg gaacgcggta agtagccgcc
      601 gtgatctgtg ccggagtatt caatggcacg gtcgacatgg gaatatcccc ccattcccgc
      661 accaggtcga aaagtagcat cgcccggagg aatttggcct ccgccgtata cgtcggcttg
      721 agactatcgg taaaggttac cttatcgatc cccccaagca gggtattgca ctgtgcaatg
      781 gcgttataca tctgcttcca gtggctatag aggtaagaat tggtcggtag cagggagaaa
      841 ttgttgaact ggaaaggttc acccgcattg gactggttat cattggtacc cgtatcatcc
      901 gatcgctgat cggtgaacag atcgctctcc tcgcccatcg cattgttgtt cttcatggcg
      961 gaataacacc cattcaacgc gagcaaaaca tcggtctccg aagtaaggaa ctgggggatc
     1021 gtcaccgcat tggggttgga taactccagg aaacttttct ggcaggagca gaacaacacc
     1081 agccccatac acatcatttc aatgaattta tttctcatcg tattgatttt agaaggttac
     1141 atgaacaccc atattataag ccctcacaag aggatagact ccatagtcga cacccggcgt
     1201 caggttggcc gtgctgctgc cgacgctgta gttataatcc acttcagggt tatatccctt
     1261 gtatttggtg atcgtaaaga cattgttcac cccggcatag acgcggatag cactaagccc
     1321 ggccctgtgc aggaccgaaa tatccggcag gttatacccg atcgagatat tggtacaccg
     1381 caggaagctg ccgtcctgca ggtagaaggt cgaaagccgg gtactattgc tctgcgtacc
     1441 ggcacgcgag gcgcgataca catgcccatt cccgggctgc gattccgacc ggtaccgctg
     1501 cgatacgact tcgtactgat tacccgaccc ttccatattg aagaggtaat aatcctggcc
     1561 atccagcacc tggttgccat aggaaccggc aaaactggca ctggcatcga tagccttgta
     1621 actggccgac aggctgaacc cataggtaaa tttgggatat ggcgacccga tgatgctcag
     1681 gtcattggca ttgacaacgc catctccatt gacatccttg aaatacaggt cacccgggcg
     1741 aagcggattg gtctgcgcaa gagaacgggg cggacctttc aatacatatc ccttggggaa
     1801 agtttgcccg ttcgcactgt aaaccttggt gtccgcattg atattggccg tatccgatgc
     1861 ccgcaccata ccggcgacct tatatccata gaacatcccg atcggctgac ccgtttccgt
     1921 gatatgcgtg atatactgcc gctcggcgcc atcactgatg atcgtactgg ccccgccaag
     1981 attgagcact ttgttccggt tgatcgaaat attgccgctt acattgaagt tgaaaccacc
     2041 ggagctctgg atcacctttc catccacctg gatatcgaag ccctggttcc tgatccgcga
     2101 attccgcagg ttggtcagga tggaggtaaa cccggacacc gcggaaatat tattgttgac
     2161 cagcagattg aaactccggc tgtcgtagaa gttggcgatc agctggatcc ggtcgtggaa
     2221 catgcccagg tcgagcccca ggttgtattg ggacgtggat tcccactcca ggttcgggtc
     2281 ctgtacattg ccggtgatat atgcctgcga gatcacgttg ttgccaaaga cgacacccgt
     2341 tgaactatag gtctggacgg tattgtaatt cccgatattg ttgttcccgc taagccccca
     2401 ggaagcccgg agacgcagcg tggtatgttc ttccagccag cccttataga aattttcctt
     2461 cgagatgttc cagccgccgc tgacagaggg gaacacggcc catttgttgt gaaggccaaa
     2521 acgggaggaa ccatccgtac gaatggatcc cgtcaggtaa tacttgccct cgtaattgta
     2581 ctgcgcccgg ccaagatagg agttcagcgt atacgagctc ttgccggttc cactgttgat
     2641 cgtgatctgt ccgggtgtac ccgccgtcac ctgggggatc aggtcgttct ggaagccttt
     2701 ggccgtgatc gacaacagat cgctgctcgt cgactgaacc gtatacccgc ccaggatatt
     2761 gatcgcatgc ggcccgaaat gcttttcata gttgagcgtg aactccgcca gctcgtcctt
     2821 ctggttgagc tgctgggcca ccgcattcgc cgccgcgatc gacgccgcgg aatagggtgg
     2881 gttggtaccg ctactcagcg aagtgggcag gtaataatca tatttctcgc cataggtctg
     2941 caggccaaga ttggtcttga aaagcagatg cgggatcagc tcgtaagtgg caaaaccatt
     3001 gtaagtggcc cggttaccct gacgcttgat ctgggttaac tcagccgtgg caatcgggtt
     3061 ttcgatagac tgatacccgt attgcgccga ctcggcatcc gcaaggtatt gcgcaatgct
     3121 gccatcgggg ttatacgcgg ggaacacggg catatagatc agcgcgccaa aagccgggct
     3181 atgatcccag cggccttcct gggtctcgtg gttggtattg cccgtatagg aaatgctggc
     3241 cccaaccttc aatttatcat tcacatcccc gtcgacattg gcgcggaagt tgacccgctg
     3301 ctgggccgtg ttcagcatga tcccatcctg gctctggtaa tccccgctga tcatataccg
     3361 catattctgc gtcccgccgg aaaacgccag gttatggcgc atgaacggcg cattccggta
     3421 cagctcattc tgccagtcgg tattgtattt ctgcgggatc tccttgcccg tggtaaaatc
     3481 atacagctcg gtgggaatac tgatgctggc cccgttgcca acccgcgcaa cacgcgtggc
     3541 attgttatcg aggtacatat tgtcgttcca gaccagcccc tggttctgta caagatcctt
     3601 atagctgtcg ttgcgcccat cgatcaccag ttgcgcaaat tggtcgccat tcagcagttt
     3661 caccttatgc gccaactgat cgacaccata ttgatagtcg tattccagct ttcctttgcc
     3721 cggtctcccc cgcttggtcg tgatcagcac gaccccgccg gccgcgcgcg atccataaat
     3781 ggccgcggaa gcggcatcct tcaggatatc gatcgactcg atatcattgg ggttgatata
     3841 aaggtcattg ccggccggat aaccgtcgat aacatacaac ggatcggtgc tggcattaaa
     3901 agaaccaaca ccccggaccc ggatcttggg gctggcatac ggcgaaccat cggtctgcga
     3961 taccaccacc ccggccacct tacctaccag tgcctgaccg gcggtaggag cggatacgtt
     4021 cagctcactg gccttgacac tcgcaaccgc accggtaaag tcgctcttcc gcatcttctg
     4081 ataccccaca aaaaccacct cattcaacgc atcacgcccc tgatgcatcc ggatatccaa
     4141 cggactcacg cctttcacca gcacatcctg cgtgccgtaa ccgacagaag acacctcgag
     4201 atgctccccg gccatcacca taatggaata atgaccgtta tcatcggtca ccacggcatt
     4261 ctgcgtccct ttcacatgca ccgtggccga acggacaggc tgcccgttct catcggccac
     4321 aagcccttcg atccgccggc tgccggccat cggggcatcc gtcgcggcat tcagcggaac
     4381 atatttcagg gtaaggttcg acccatcgag cagataatgt aacccatacc gcttctgcag
     4441 gatctccagc gcatcttcca ggcgctgcgc cttcaattga agccgatcca ccttcacttt
     4501 ctccaggact tcctggctat aggtaaaatt caccgtgggc gcctgctgct gcagggccgc
     4561 aacgacttta ctcagatccg atccacgcag atcgagtcgg atggtctgag cccaccccct
     4621 ctgccccagc aacaggaaca aaaagagcaa cccacacccc ataaagctgc tttgtaattt
     4681 tctggtcata ccttatgaat tgtttgtttt agttatctga catgtaaagt atctccgttc
     4741 cggacaaaat gcaggtccca gccaaaggac agggattgca gcactttctc cagtgccgta
     4801 ttatcaaacc ttgccgtaat ggtcctgtcc gttatcgcat gatcgtaaat gatgtgtacc
     4861 ccatagcgcg cctcgatctg cgccagcgcc tccttcaacg gcgttttgta gaaaagcatc
     4921 cgccccgccg tccagacatc catttccttc ggcgcctgct gcagtacctc tccattaccc
     4981 gactccttat catataccat cagttggccc ggcagcaaca ccttttgcgt cttattccca
     5041 tactcgaccc cgatcttgcc gctcttgagc gaaatacgca gctcaccggc ctcgggatag
     5101 gcggatatgt tgaaggaagt gccaaagaca tgggtggccg tgttcccggc gatgacgaca
     5161 aagggatgat gaacatcgga tgcaacctcg aaaaatgcct cgccgctgag ccggacctcc
     5221 cggtgcgtgg caaaatccgc cgggaaggat acatgggtcg cggagttgag ccagacgatc
     5281 gaactatccg gtaacaacac cttgcgcacc tgctgatagg ccgtgccgat cgtcgtatag
     5341 gtcgacgcct tcctcgcgat actccttcca ccccgccggt tccactgcat ccagatccca
     5401 ccggagaaca tgatcattcc cgcccagacg gcggcatagc gcaacatccg ccggtgacga
     5461 agccggttct cggggtcctt ccctatctcg ggattctccc tggcagcgat ccgatcccac
     5521 aactcctgca caagacgctg ttcctcgacc tctgacaaca atcgc
//
LOCUS       DATGFG010000582         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_979944, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000582 DATGFG010000000
VERSION     DATGFG010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979944"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VKQ52_10890"
     CDS             complement(<1..199)
                     /locus_tag="VKQ52_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87742.1"
                     /translation="MNFIDDIPPPAGSSDPTGDLELIAAYRQSSDLKIVAQLYDRYLQ
                     LLYGVCLKYLGERETAKDAVMD"
     gene            complement(250..1152)
                     /gene="xerD"
                     /locus_tag="VKQ52_10895"
     CDS             complement(250..1152)
                     /gene="xerD"
                     /locus_tag="VKQ52_10895"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HLZ87743.1"
                     /translation="MWEPYKKGYKAWLQLERSLSDNSVEAYLRDIDKLTQWLQAAGTL
                     KSPDAIELGDLQEFLHWIAGLGLNAASQARILSGIRSFYKYCFIEQIVRKDPTTLLES
                     PKLKRALPDVLSFDEIESIIGRIDLSKPEGGRNKAILETMYSCGLRVSEVVGLRISCL
                     YLDAGYIRVIGKGDKERLVPVGDAAIKYIRIYQQDIRIHVPVKPGSEDILFLNRRGSK
                     LTRVMIFLFLRDLVRQAGITKNISPHTFRHSFATHLVEGGADLRAVQEMLGHESITTT
                     EIYTHLDREYLRETLHRYHPAFRH"
     gene            1341..1793
                     /locus_tag="VKQ52_10900"
     CDS             1341..1793
                     /locus_tag="VKQ52_10900"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HLZ87744.1"
                     /translation="MKGFLSRLEKKRPKGFRDLEAEANKEVWERTDCLDCANCCKTMS
                     PTYTPEDIRRISAHLNMTPEAFREKWLYKDKTGDWMNVKQPCQFLDLKTNMCDIYAVR
                     PRDCAGFPHHVKKPMNADYMHMYKQNIEYCPATYRLVETVMEKLNGHK"
     gene            complement(1804..2160)
                     /locus_tag="VKQ52_10905"
     CDS             complement(1804..2160)
                     /locus_tag="VKQ52_10905"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HLZ87745.1"
                     /translation="MLSKAEVGAEGERLAAQWLTQQGFTILKRNFRHGRYEVDIIAGR
                     NAVLHFIEVKCRRSTAYGHPEQSVSRKKLEHVLQGGAGWLAQWPGHRRVQYDVLAITL
                     RRPAPPEYILFEDVYV"
BASE COUNT          516 a          586 c          715 g          555 t
ORIGIN      
        1 tgtccatgac ggcgtctttc gccgtttccc gttccccgag gtatttcaga cagacgccat
       61 aaagcagttg caggtagcga tcatacagtt gcgccactat tttcaggtcg gacgattgcc
      121 ggtaggcggc gatcagttcc aggtcgccgg tcgggtcgga gctgcctgca gggggtggta
      181 tgtcgtcgat aaagttcaaa ttatacttcc ggtccgtctt gtgaacaaag taagaaaaat
      241 ttcgggaact caatggcgga atgccgggtg atagcggtgg agggtctccc ggagatattc
      301 gcggtcgagg tgggtgtaga tctcggttgt cgtaatgctt tcatgaccca gcatttcctg
      361 tacggcgcgc agatctgccc cgccttccac caggtgggtg gcaaaggagt ggcggaaggt
      421 gtgcggggag atgttcttgg tgataccggc ctggcggacc aggtcccgga ggaagaggaa
      481 gatcatcacc cgcgtgagtt tactaccccg ccggttgagg aacaggatgt cctcgctgcc
      541 gggtttcacc ggtacatgta tgcggatatc ctgctggtag atgcggatgt attttatcgc
      601 ggcatcgccc accggcacca gtcgttcctt atcccctttc ccgatgacgc ggatatagcc
      661 ggcatccagg tagagacagg agatacggag accgacgact tcgctgaccc gaagcccgca
      721 gctgtacatc gtctcgagga tggccttgtt gcgtccgcct tccggcttgc tcagatcgat
      781 ccggccgatg atggattcta tctcgtcgaa actgaggacg tcggggaggg cccgcttcag
      841 tttgggggat tccaggaggg tggtggggtc cttgcggacg atctgttcga taaagcagta
      901 tttgtaaaag gagcggatgc ccgagaggat acgggcctgg gaggcggcgt tcaggccgag
      961 ccccgcgatc cagtggagaa actcctggag atcgcccagt tctatggcgt cgggagattt
     1021 tagtgtgccg gcggcctgga gccattgggt cagcttgtcg atgtcgcgga gataggcttc
     1081 gacggagttg tcggagagcg atctttccag ttgcagccag gctttgtagc cttttttata
     1141 gggctcccac atagctcgaa gataaataat gtgcgggaga tgcactgtcg ggaaattagc
     1201 gcatctcccg caaaacgcta atatttggcc ttcggccgac ggacctccgg tccgttttga
     1261 atactatcct atatttgcag cctatgacga cagttaatct acgttccttc agacagatcg
     1321 tgcttcataa caagaggcgg atgaaaggtt ttttaagccg gttggagaag aagcgaccga
     1381 aagggttccg cgatctcgag gcggaggcga ataaggaagt ctgggaacgg accgactgtc
     1441 ttgactgtgc caattgttgt aagacgatgt cccccacgta tacgccggag gatatccggc
     1501 ggatctcggc gcatctgaac atgacgccgg aggcgtttcg cgagaagtgg ttgtacaagg
     1561 acaagacggg ggattggatg aatgtgaaac agccctgtca gtttcttgac cttaagacca
     1621 acatgtgcga tatctatgcc gtcaggcccc gcgattgcgc gggcttcccg catcacgtca
     1681 aaaagcctat gaatgcggac tatatgcaca tgtataaaca gaatatcgag tattgccccg
     1741 ctacttaccg gttggtagag accgtgatgg agaagctcaa cggtcacaag taaacgtctt
     1801 cggtcataca tagacgtctt caaaaagaat gtattcggga ggggcagggc gccggagcgt
     1861 gatggcgaga acatcatatt gcacccggcg atgccccggc cattgcgcca gccagcctgc
     1921 tccgccttgc aggacatgtt ccagcttttt ccggcttacg ctttgttcgg gatgcccgta
     1981 agcagtggag cggcggcact tgacctcgat gaagtggagg acggcattac ggcccgcgat
     2041 gatgtccact tcataacggc catggcggaa attgcgtttt agtatggtaa agccttgttg
     2101 ggtcagccat tgtgccgcca gtctttcccc ttctgccccc acttctgcct tcgacaacat
     2161 cttgtatgaa ttttcattat tttgctgcgc gaaaagagga ataacatgga aaaaggccgg
     2221 aaggttttca aattacaggg gaaagttcag cattatgcct ggggtggggc agcctatctg
     2281 ccgcgtcttc tgaatatccc caacccggag gggcgtccat ttgccgaata ctggatgggt
     2341 gcacatgata acgcaccttc ggaactggat gg
//
LOCUS       DATGFG010000583         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_580910, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000583 DATGFG010000000
VERSION     DATGFG010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580910"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VKQ52_10910"
     CDS             complement(<1..625)
                     /locus_tag="VKQ52_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HLZ87746.1"
                     /translation="MQLKSIVAGWMLVAAVQRLPAQMSVNGVVYVNTYKALAMSEMQR
                     SGIPASIILAQGLHESEAGTSELVKQSNNHFGIKCKDDWKGQVVYHDDDARQECFRSY
                     ASAADSYRDHSDFLRRSSRYAFLFQLDPADYEGWAYGLKKAGYATNIRYSQILIKLIK
                     QYNLQQYTLIAMGRLKPSEEVVLTMPGAPAVTTVAATAVETPVVVTKN"
     gene            complement(632..1270)
                     /locus_tag="VKQ52_10915"
     CDS             complement(632..1270)
                     /locus_tag="VKQ52_10915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HLZ87747.1"
                     /translation="MEIIDPLAQAYAERYSSKLSPLLCDIAAYTAAHHPEAHMLSGPL
                     QGKLLELVSHLLRPRRVLEIGTFMGYSALCLASGLTGDGKLHTIELREEDADRAEENF
                     RRANWLDRIILHRGNALGIIPALEEVWDIVFIDADKVGYEAYYQLVLPKVRSGGLIIA
                     DNVLFHGGVLADEIKGKNAKAIQAFNEVVAADERVEKVMLTVRDGLFFIRKK"
     gene            complement(1349..2848)
                     /locus_tag="VKQ52_10920"
     CDS             complement(1349..2848)
                     /locus_tag="VKQ52_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HLZ87748.1"
                     /translation="MSSFLLFAFVIGYFLLLLVVAWRTSRHSNNDSFFIGNRNSNWML
                     VAFGMIGTSLSGVTFVSVPGTVGDWSGDVHKAFGYFQVIIGYWFGYLVIAFVLIPLYY
                     RLHLTSIYNYLHTRFGPVAYRTGALFFIISRTVGATARLYLVINVLHIFILKDLGIPF
                     GLTSLVILLMILLYTFEGGVKTIVYTDTLQTTFMLLGLCVCTVYILQNLHLSFGGALQ
                     AMGARGFTHIFNGDAGSKGFWGKQVLGGMFIAIAMTGLDQEMMQKNISVRNVRDSKKN
                     VMTFSFIMVLVSFLFLLLGGLLYLYSLQRGAVYQGRALLLGGRNVIGDDLFPTLALHY
                     LPAAVSIIFIIGLISALFPSADGALTALTSSFCIDLLGLRDRTDLSEAQKKRTRLTVH
                     VSFALVFLLCILVFKWVDNKSIVNTVLELAGYTYGPLLGLFAFGIFTKRVLPNTWKIT
                     ALCLLAPVACYFLSQNSGRWFGGFQIGLELLVINGLLTFLGIWALSKRA"
BASE COUNT          809 a          955 c          661 g          570 t
ORIGIN      
        1 cattcttcgt caccacaacg ggcgtctcca ccgccgtcgc cgccaccgtc gtcaccgccg
       61 gcgcaccagg catggtaagc accacctcct ccgacggctt cagtctgccc atcgcgatca
      121 gcgtatattg ctgcaggtta tactgtttta tcagtttgat caggatctgg gagtatcgga
      181 tattcgtagc ataccccgcc ttcttcaacc cataggccca tccctcataa tccgcggggt
      241 ccagctggaa aaggaaagca tatcgcgaac tccggcgaag gaaatccgaa tgatcccggt
      301 aagaatcggc cgcggaggca taacttcgaa aacattcctg ccgggcatca tcgtcatgat
      361 acaccacctg ccccttccag tcatccttgc atttgatccc aaaatgattg ttcgactgct
      421 ttaccagttc actcgtgccc gcttccgact catgcagccc ctgcgccagg atgatcgacg
      481 ccggtatccc cgaccgctgc atctccgaca tggccagcgc cttgtaggtg ttgacataca
      541 ccaccccatt cacactcatc tgcgccggca accgttgcac cgccgccacc agcatccatc
      601 ccgctacaat actttttaat tgcatccttt attatttctt ccggataaag aacaacccat
      661 cccggaccgt aagcatgact ttttctaccc gctcatccgc cgccaccact tcattaaagg
      721 cctggatcgc cttcgcattc ttccccttta tctcatcagc caaaaccccg ccatgaaaca
      781 gcacattgtc ggcaatgatc agtcccccac tccgcacctt cggcaatact aattgataat
      841 aagcctcata tcccacctta tcggcatcta taaaaacaat atcccagacc tcttcaaggg
      901 ctgggatgat gcctaacgca tttccccggt gcaatattat ccggtccagc cagtttgccc
      961 ggcggaaatt ctcctccgcc cggtcagcat cctcctcccg cagttcgatg gtatgcaact
     1021 tcccatcccc cgtcagaccc gatgccaggc aaagcgcgct gtaccccata aaagtcccga
     1081 tctccaaaac ccgccgggga cgcagcaaat ggctcaccag ctccagcagc ttcccctgca
     1141 aagggccact cagcatatgg gcctcggggt gatgcgccgc cgtatacgcc gctatatcgc
     1201 acagcaaagg actcagcttc gaagaatacc gttcggcata tgcctgcgcc agggggtcga
     1261 ttatctccat tccggtaaaa tttaatttcg gtcaccgggc tccgccggtt tttgttgcgc
     1321 aaagatacgc cttccggcat ctcggcgcct atgcccgctt cgacaacgcc cagatcccta
     1381 aaaaggtcag cagaccattg ataaccagca gttcgagccc gatctggaaa cccccgaacc
     1441 agcgcccgga gttctggctg agaaaataac aggccaccgg cgccagcagg caaagcgccg
     1501 tgatcttcca ggtattgggc agcacccgtt tcgtaaagat gccaaaggca aaaagcccca
     1561 gcagggggcc atacgtatac cccgccagct cgaggacggt attgacgatg gatttattat
     1621 ccacccactt gaacacaaga atgcaaagca ggaataccag ggcaaaactg acatgcacgg
     1681 tcagccgcgt acgctttttc tgcgcctcgc tcaggtcggt cctgtcccgc aaccccagca
     1741 gatcgataca aaaggacgag gtcagggccg taagcgcccc atccgcactg ggaaaaagag
     1801 ccgagataag cccgatgata aaaatgatcg acaccgccgc cggcagataa tgcagggcca
     1861 gggtcgggaa cagatcatcc ccgatgacat tccgcccccc aagcaacaac gcacgcccct
     1921 gatacaccgc tccccgctgc aacgaataca ggtacaacaa cccacccagc aacaggaaca
     1981 aaaagctcac cagcaccatg ataaaactaa aggtcatcac attcttcttg gaatcccgga
     2041 cattcctcac actgatattc ttctgcatca tctcctggtc cagccccgtc atcgcaatgg
     2101 cgataaacat cccgccaagc acctgcttcc cccaaaaacc cttgctgccc gcatccccgt
     2161 taaagatatg cgtaaagccc ctggccccca tcgcctgcag ggccccgcca aacgaaagat
     2221 gaaggttttg cagaatatac acggtacaga cacaaagccc cagcagcatg aaggtagtct
     2281 gcagcgtatc ggtatacacg atggtcttca caccgccctc aaatgtatac aacaggatca
     2341 tcagcaggat gaccaggctc gtcaacccga aaggaatacc gagatccttc aggataaaaa
     2401 tatgcagcac attgatgacg aggtaaagcc gcgccgtggc gcccaccgtg cgggaaatga
     2461 taaagaacaa agccccggta cggtacgcca ccggcccgaa acgggtatgc agataattgt
     2521 agatcgaagt cagatgaagc cggtagtaca gggggatcag gacaaaggcg atcaccaggt
     2581 aaccaaacca gtaaccgatg atcacctgga aatagccaaa cgccttatgg acatccccgg
     2641 accagtcgcc caccgtaccc gggacactga caaacgtaac ccccgaaaga ctggtcccga
     2701 tcatcccaaa agccaccagc atccagttgg aattgcggtt gccgataaag aaagagtcat
     2761 tgttggaatg acgcgaggta cgccaggcta ccaccagcag caacaggaaa taaccgatga
     2821 caaacgcaaa aagcagaaaa gacgacatct atcaaaaata ggaaaaatcc tatctccggt
     2881 gcaggtaaaa gtcgaaaccc gcccgtatca ccggcaacgg atgaccatag atatcattat
     2941 aaggcgaatt cttattggaa atagcgtcga acatcagcgc tatataaaac ccgct
//
LOCUS       DATGFG010000584         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_415014, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000584 DATGFG010000000
VERSION     DATGFG010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415014"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VKQ52_10925"
     CDS             <1..636
                     /locus_tag="VKQ52_10925"
                     /inference="COORDINATES: protein motif:HMM:NF019583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HLZ87749.1"
                     /translation="QQGWFVPHDIPGMVALMGGREKAIADLSEMFARTPGNMLWNDYY
                     NHANEPVHHIPFLFNRLGAPWLTQRWSRQICQRAYHNGVEGLVGNEDVGQMSAWYVLA
                     ASGFHPVCPGDTRYEITSPVFSRVVIRVGGGGSGAGLGERGGSDARGNDGGGRTFTIV
                     ARNNSPENVYIQSATLNGKPYRVCHIDHAQIVAGGRLELMMGARPNKNWGL"
     gene            633..>4051
                     /locus_tag="VKQ52_10930"
     CDS             633..>4051
                     /locus_tag="VKQ52_10930"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HLZ87750.1"
                     /translation="MKTKTGLLMIWVFFAAAATAAAQDLVHYANTLQGTASDFGLSYG
                     NTYPATALPFPMNAWSPQTGKDGDGWKYQYSADKIRAFGETHQCSPWVGDYGVFSLMP
                     VLDSAQPDPDKRATGFSHANEIGQPGYYSVKLDNGIRVEMSPTERGCHMRISYSGKAR
                     GHAAYMMIDGYIKESKVYIDAGRRRISGWVNNGRWTPAGFRNYFIIAFNRPFVSWDSR
                     GGYVQFAKGVVVEAKIASSYISPEQAEVTLERELGGFASFDATHRAADSVWNRLFRRV
                     LVEGGTEEQKATFYSCLFRASLFSHSFFEYDKDGKPWYYSPYDSRVHSGYMYTDNGFW
                     DTFRSQFPLTNILHPTMQGRYMQALLAAQQQCGWLPAWSAPSETGGMLGNHAISLLAD
                     AWVKGIRSFSPDSALKAYAHEAMNKGPWGGANGRAGWKDYWELGYVAYPESQGSTAQT
                     QEYAYDDFCAYALARMTGNTFYEKMFARQMYNYKNVFDTGTGFMRGRQENGEWTPGFD
                     PLAWGGPYTEGNAWHYNWSVFHDVQGLIGLMGGEAKFVAKIDSVFTLPDTVVYGSYGG
                     MIHEMKEMVLAHMGQYAQGNQPIQHMIYLYSYAGQPWKTQRHIREVMDRLYNATPNGY
                     PGDEDQGGMSSWYVLSAMGIYSVCPGTDQYVLGSPVFRKVTITLENGKRFVIEAANNS
                     ASNVYIRSATLNGRVYTHNWIRYNDIISGGVLHLEMSGEPDYHRGTAPDDKPFSVSTQ
                     DGGGYAAVAGIPLTLAPVLQDNMVIQQNKPFTIWGHAAPGEAVLVSADWLSGELTVYA
                     DSAGAFAAIVAVPGVKAGDYREHSLTVSCGGELKTLSHLLIGEVWLCSGQSNMQFSMA
                     TVLDSTADIAAARYPAIRLFNASLNFSDHPLEELGGSWVACSPGTVRGFSAVGYYFGR
                     RLQQTLNVPVGILFSGIGASAAQAYVPRAVLAADTLLDRRYLEPYLQGPHSHEPVDGG
                     FSFEKVTRPFLLYNAMIHPLRHLSVRGICWYQGESNRVERVSYTRLTQALIRSWRQAF
                     AQGDLPFYLVQVAPFFYDQPDSTLADYAFFREAQERVTTLANTGMVVTMDVGEARNLH
                     PLNKKPVGERLADMALNREYGYTDRVYRGPQYRWWEVAGRDAVVHFEVGTVA"
BASE COUNT          795 a          956 c         1353 g          947 t
ORIGIN      
        1 cagcaggggt ggtttgtgcc gcacgatatc cccggcatgg tagcgctgat gggcgggagg
       61 gagaaggcga tcgccgatct gagcgagatg ttcgccagga ctccggggaa tatgctttgg
      121 aatgactatt ataatcatgc gaatgagccg gtgcatcata ttccttttct tttcaaccgg
      181 cttggagcgc cctggctgac gcagcgatgg agccgccaga tctgccagcg ggcttatcat
      241 aatggggtgg aggggttagt gggcaatgag gatgtggggc agatgtccgc gtggtatgtg
      301 ctggctgcga gtgggtttca tccggtatgt ccgggggata cgcggtatga gatcaccagt
      361 ccggtgtttt cgagggttgt tatccgggtg ggcggtggtg gcagtggtgc ggggttgggt
      421 gagcgcggcg gcagtgatgc gagggggaat gatggcggtg gcaggacgtt tacgatcgtt
      481 gcccgcaata atagtccgga gaatgtttat attcaatcgg cgaccctgaa tgggaagcct
      541 taccgggtgt gtcatatcga ccacgcgcag atagtagcgg gggggaggct ggaattaatg
      601 atgggcgccc ggcccaataa aaattggggt ttatgaaaac taagacagga ttgctgatga
      661 tatgggtgtt ttttgctgct gcggcgacgg cggcggctca ggacctggtg cattatgcca
      721 ataccctgca gggtacggcg tcggactttg ggttgagtta tgggaatact tatcctgcca
      781 ccgccctgcc ctttcccatg aatgcttgga gcccgcaaac gggcaaggat ggggatggct
      841 ggaaatacca atattcggcg gacaagatcc gcgcctttgg cgagacgcat caatgcagcc
      901 cctgggtggg cgactatggc gtattctcgc tgatgccggt actggacagc gcgcagcctg
      961 atcccgacaa acgggctact ggttttagtc atgcgaatga gatcggccag cccggctatt
     1021 atagtgttaa actggataac ggtatccggg tggagatgag ccctaccgag cgaggctgtc
     1081 atatgcggat ctcgtattca ggaaaggcaa gggggcatgc cgcgtatatg atgatcgacg
     1141 gctatatcaa agagagtaag gtgtatatcg atgcaggccg tcgcaggatc agcggatggg
     1201 tgaataatgg caggtggaca cccgcgggtt ttcgaaatta ttttattatt gcatttaaca
     1261 ggccgtttgt gtcgtgggat agtagaggtg gctatgtgca gtttgcgaag ggggttgtcg
     1321 tggaggcgaa gatcgcgtcg tcgtatatca gtcctgaaca ggcggaagtg actttagaac
     1381 gggagttggg cgggtttgcc tcttttgacg ccacgcatcg ggcggcggat tcggtttgga
     1441 accggctttt tcgccgggtg ctggtggaag gcgggacgga ggagcagaag gcaaccttct
     1501 actcctgtct ttttcgcgcc agtcttttct cgcatagctt tttcgagtat gataaagatg
     1561 gcaagccatg gtattatagt ccttatgata gccgtgttca tagcgggtat atgtatacgg
     1621 ataatggttt ctgggatacg ttccgctcgc agtttccgct gacgaatatc ctgcatccta
     1681 cgatgcaggg gagatacatg caggcgttgc tggcggcaca acagcagtgc ggctggctac
     1741 cggcctggtc cgcgccttcc gagacgggcg gcatgctggg caaccatgcc atctcgctgc
     1801 tggccgatgc gtgggtgaag ggcattcgca gttttagtcc cgactccgcg ctgaaggcct
     1861 atgcgcatga ggcgatgaat aaagggccat ggggtggagc gaatggccgc gccggctgga
     1921 aggattattg ggagttggga tatgtggcct atcccgaatc ccaggggtcg accgcgcaga
     1981 cccaggaata tgcgtacgat gatttctgcg cctatgcttt ggcgcggatg acagggaata
     2041 ccttttatga gaagatgttt gcgcggcaga tgtataatta taagaatgta tttgataccg
     2101 gtaccggttt tatgcgaggg cggcaggaaa atggggaatg gacgccgggt tttgatcccc
     2161 tggcctgggg cggtccctat acggagggga atgcgtggca ctacaattgg agtgtgttcc
     2221 atgatgtgca gggactgatc gggttgatgg gtggtgaggc gaagtttgtg gcgaaaatcg
     2281 attcggtgtt tacgttgcct gataccgtgg tatacggcag ctatggcggc atgatccatg
     2341 agatgaaaga gatggtcctt gcccatatgg ggcagtatgc ccagggcaac cagcctatcc
     2401 agcatatgat ctacctgtat agctatgccg gccagccctg gaagacccag cggcatatcc
     2461 gcgaagtgat ggaccggttg tacaatgcga ctccgaacgg ttatcccggg gatgaggacc
     2521 agggcggcat gtcttcctgg tatgtactga gcgcgatggg catctacagt gtgtgcccgg
     2581 gcaccgacca gtatgtgttg ggcagtcctg tcttcaggaa ggtgacgatc acgctggaga
     2641 atggcaaacg gtttgtgatt gaagcggcga ataatagtgc ttccaacgtc tatatccgat
     2701 cggccacgct gaatggccgg gtgtatacgc acaactggat caggtataac gatattattt
     2761 cgggcggcgt gctgcacctg gagatgtccg gcgagcccga ctaccatcgt ggcaccgcgc
     2821 cggatgacaa gcctttttcc gtgtcaacgc aggatggtgg gggttatgct gccgtggccg
     2881 ggatcccgct gacccttgcc ccggtgttac aggacaatat ggtgatccag cagaacaagc
     2941 cctttacgat ctgggggcat gcggctccgg gcgaggcggt tttggtgtcg gccgactggc
     3001 tttccggtga gctcaccgta tatgccgaca gtgccggcgc gttcgcggcg atcgtggctg
     3061 ttccgggtgt caaggcgggg gactatcggg agcattcgtt gaccgtcagc tgcggcggcg
     3121 agctgaagac cttgtcgcac ctgctgatcg gcgaggtctg gctgtgtagt ggccagtcga
     3181 atatgcagtt cagcatggcg acggtgctgg attcgaccgc ggatatcgcc gccgcccggt
     3241 accctgctat ccgcctgttc aatgccagcc tgaatttcag tgatcatccg ctcgaggagc
     3301 tgggcgggag ctgggtggcg tgctcgcccg ggacagtgcg cggattcagt gctgtggggt
     3361 attatttcgg gcggcggttg caacaaacat tgaatgttcc cgtggggatc ctcttttcag
     3421 ggatcggggc ttctgccgcg caggcctatg tgcccagggc tgtattggcg gcggataccc
     3481 tgctcgacag gcgctacctc gagccctatc tgcagggccc gcattcccat gagccggtgg
     3541 acggcggttt ttcgttcgag aaggtgaccc ggccatttct cctgtacaat gcgatgatcc
     3601 atcccttgcg tcatttgtcg gtgcgcggca tctgctggta ccagggcgaa tccaaccggg
     3661 tggaacgagt gtcctatacg cggctcaccc aggccctgat ccgcagctgg cggcaggctt
     3721 ttgcccaggg cgatctgcct ttttacttag ttcaggtggc gccgttcttt tacgaccagc
     3781 ccgattcgac gctggccgat tatgcttttt tccgggaggc gcaggaaagg gtcaccaccc
     3841 tggcgaatac cggcatggtg gtcacgatgg atgtcggcga agcgaggaat cttcaccccc
     3901 tgaataaaaa acctgtcggt gaacgccttg ccgatatggc gctcaatcgc gaatatggat
     3961 atacggatcg ggtataccgt ggtccgcaat accggtggtg ggaagttgca ggccgggatg
     4021 ctgttgtcca tttcgaagtg ggaacggtgg c
//
LOCUS       DATGFG010000585         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_376461, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000585 DATGFG010000000
VERSION     DATGFG010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376461"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VKQ52_10935"
     CDS             complement(<1..394)
                     /locus_tag="VKQ52_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87751.1"
                     /translation="METYRMEPGRIPFASFLSTAFDKGDYSTDDVLAFVLPLFRKVLG
                     FHEAGMVAPFDREGALFVHAGVLDIDETLAHAPADALYRVNALFPREPAGPPPSYLPG
                     YRSFEHPAGHHDPQTDIYTLGLILGNMAM"
     gene            complement(399..1343)
                     /locus_tag="VKQ52_10940"
     CDS             complement(399..1343)
                     /locus_tag="VKQ52_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87752.1"
                     /translation="MLPEALPYHLKVRDHLQGQASTWEFFSAARTREEQEGSLQPELL
                     KDCRPLTREKDAELYAQIDLARERLGLAAIGVTACEEEAANAAARIIFLRRQAFLVLA
                     GPVLESTAAERLAVIARALSLVRLYTLEDGTLEIADRIVTAMAHHPASDASCRETARL
                     FQLHTALFCDRGVLAVTGDGVDGSGEQGEQGVARPGEGTADLNRLDMNRLDLFGQAAL
                     YALTREILWELLQPEEFRTDLTLRMAHQYFPDLSWPGAGTGAVRERLVATLSGAAVNV
                     HDYFSYLLQDIAQADPSLAVRLGERVRAFAEETQLSVK"
     gene            1382..>2907
                     /locus_tag="VKQ52_10945"
     CDS             1382..>2907
                     /locus_tag="VKQ52_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLZ87753.1"
                     /translation="MRDYISELNDRQKEAVLHMDGPIMIVAGAGSGKTKVLTTRIAHL
                     LAQGVSPWNILALTFTNKAAKEMKERVEKILGNSDARNLYIGTFHSVFARVLRGEAER
                     LGYPRNFTIYDTDDAKSVLKTVINELNLDDKHYKPNTVYNRISQAKNSLVGPADYAND
                     YALQQEDMRSNRPAIAQIYDAYAKRCFKNGAMDFDDLLIKMYELLKNFPDALHKFQHK
                     FKYIMIDEYQDTNPAQYEVIKLLGAAHENVCVVGDDAQSIYSFRGATIENILQFQKDY
                     DDVKVVKLEQNYRSTQSILNVANEVIRNNKGQIPKNLWTDNAPGEKIRLVRMMTDNDE
                     GKFVADTIQEQRLRNHYNNRDFAILYRTNAQSRAFEESLRRMAIPYTMYGSISFYSRK
                     EIKDFVAYLRIIVNSRDEESLKRIINYPTRGIGKTSIDRAVLAANENNISLWEVLEKA
                     AQFGFRAGTLEAIENFVLMIRSFTSMLEKHNAYEVAAHLGKQTNLIKDLFNDKSTEGV
                     A"
BASE COUNT          735 a          841 c          808 g          523 t
ORIGIN      
        1 ccatcgccat gttgccaaga ataagcccca gggtataaat atccgtttgc gggtcatgat
       61 gccccgctgg atgttcgaag ctgcgataac cgggaagata ggaaggggga ggaccggcgg
      121 gttcccgggg gaaaagcgcg ttcacccggt aaagggcgtc ggcaggggcg tgcgccagcg
      181 tttcgtcgat atccaggaca ccggcatgga caaaaagcgc gccttccctg tcaaaaggcg
      241 caaccatccc ggcttcatga aaaccaagca cttttctgaa tagcggcagc acgaatgcga
      301 ggacatcgtc cgtcgaatag tcacccttgt cgaaggcggt ggacaagaag ctggcaaaag
      361 ggatgcggcc tggttccatg cgataggttt ccataggttt attttactga caattgggtc
      421 tcttccgcaa aagcacgcac ccgttccccc aaccggactg ccagcgaggg atcggcctgc
      481 gcgatatcct gcagaagata ggaaaaatag tcgtggacgt tgacagccgc gccggaaagc
      541 gtcgcgacca gccgttcgcg gacagcgccg gtcccggcgc ccggccagga taagtccggg
      601 aaatactggt gcgccatccg cagcgtaaga tcggtgcgga actcctcagg ctgcagcaat
      661 tcccataaga tctcccgggt aagggcatag agcgctgcct ggccgaacag gtccagccgg
      721 ttcatgtcga gccggttcag gtcggccgtt ccttcgcccg gccgggcgac gccctgttcc
      781 ccctgctccc cgctgccgtc gaccccatca ccggtgaccg caagaacacc acggtcgcag
      841 aagagggcgg tatgcaattg gaaaagtctc gctgtttccc ggcaggacgc atcgctggcc
      901 ggatgatgag ccatcgcggt gacgatccgg tccgcgatct ccagtgttcc gtcctcgagg
      961 gtatacagac gcaccaggct caacgcacgg gcgatgacgg ccagccgttc ggccgcggtt
     1021 gattcgagca ccggcccggc aagaacgaga aaggcctgcc gccggagaaa gatgatccgc
     1081 gcggctgcat tcgcagcctc ctcctcgcag gcggtgacgc ctatcgctgc gaggcccagc
     1141 ctctcccggg caagatcgat ctgcgcataa agctccgcat ccttttcccg cgtcagcgga
     1201 cggcaatcct tcaataattc cggctgcaaa gaaccctctt gctcttcgcg ggtacgggca
     1261 gcagaaaaaa actcccaggt ggaagcctgc ccttgtaaat ggtcgcggac cttgagatga
     1321 taaggtagag cctcgggtaa catagatcac gtaaatctac ggatttaagt aacttcgcga
     1381 catgcgagat tatataagcg aattgaacga ccggcagaaa gaagcggtat tacatatgga
     1441 cggtccgatc atgatcgtgg caggcgctgg aagcggtaag acaaaggtgc tgacgacgcg
     1501 catcgcccac ctgctggcgc agggggtgtc tccgtggaac atcctggcgc ttacctttac
     1561 caataaggcg gcaaaagaga tgaaggaacg cgtggaaaag attctcggca acagcgacgc
     1621 ccgcaactta tacataggaa cttttcatag tgtgttcgcc cgggtgctaa ggggcgaggc
     1681 cgaacggctg gggtatcccc ggaatttcac catttatgat acagatgacg ccaaaagcgt
     1741 gctgaagacc gtcatcaatg aattgaacct cgacgacaaa cattataaac ccaatacggt
     1801 ctataaccgg atctcacagg ccaagaacag cctcgtcgga ccggcggatt acgccaatga
     1861 ctatgccctc cagcaggaag atatgcgcag caaccggccg gccatcgcgc agatctacga
     1921 tgcctatgcc aaacgttgtt ttaagaacgg ggccatggac ttcgacgacc tgctgatcaa
     1981 gatgtacgaa ctgttaaaga acttccccga tgccctccat aagttccagc acaagttcaa
     2041 atacatcatg atcgatgagt accaggatac caacccggcc cagtacgaag tcataaaatt
     2101 gctgggcgcc gctcatgaga atgtctgcgt cgtcggcgac gacgcccaga gcatttatag
     2161 tttccgcggg gcgaccatcg agaatatcct ccagttccag aaggactatg acgatgtaaa
     2221 ggtggtaaag ctggaacaga actaccgcag tacccaaagc atcctcaatg tcgccaacga
     2281 ggtcatccgc aacaacaagg gccagatccc aaagaatctg tggacggaca acgcccccgg
     2341 cgaaaagatc cggctggtcc ggatgatgac ggacaacgat gaagggaaat tcgtagccga
     2401 caccatccag gaacaacggc ttcgcaatca ctacaacaac cgcgacttcg ctatcctcta
     2461 ccggaccaat gcccaaagcc gggcctttga agaaagcctg cgccggatgg ccatcccgta
     2521 tacgatgtac ggcagcatca gtttctattc ccggaaggag ataaaggact tcgtggccta
     2581 cctgcggatc atcgtcaact cccgggacga ggaatccctg aagcgcatca tcaactaccc
     2641 aacgcgcggc atcggcaaaa cctccatcga cagggccgtg ctggcagcga atgagaacaa
     2701 tatctccctc tgggaggtgc tggagaaagc cgcgcaattc ggcttcaggg ccggcacgct
     2761 cgaagcaata gagaatttcg tactcatgat ccgcagcttc acctccatgc tggagaaaca
     2821 caacgcctat gaggtggccg cacacctcgg caaacaaacc aacctgatca aggatctgtt
     2881 caatgacaag tccaccgaag gcgtggc
//
LOCUS       DATGFG010000586         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_984251, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000586 DATGFG010000000
VERSION     DATGFG010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984251"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1230
                     /locus_tag="VKQ52_10950"
     CDS             187..1230
                     /locus_tag="VKQ52_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HLZ87754.1"
                     /translation="MTDLSHFDSNGPSNPKNNIFGLPFSEDDARLVVLPVPWEVTVSY
                     GAGTSRACEHVFHASMQVDLFDGETGDAWRQGLYMRQPDKKVLMKSDYLRKEAELYIN
                     FTSKDEPVEKNSFMCKSLKEINEGSASLNKWVYEQTKGLLDQGKLVALLGGDHSTPLG
                     YFKAIAEKHGDFGILQIDAHCDLRKCYEDFVYSHASVMYNALQEIPQLKRLIQVGVRD
                     YSLDEWEFIQSSNYKVITYFDKDIKERGYEGQTWRQIADEIVSHLPEKVYISFDIDGL
                     DPKLCPHTGTPVQGGFEIEQIFYLIRKIIQSGRQFLGFDLVEIGVGDTDWDANVGARI
                     LWKLSNLLVASNS"
     gene            1249..1869
                     /locus_tag="VKQ52_10955"
     CDS             1249..1869
                     /locus_tag="VKQ52_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87755.1"
                     /translation="MRYQYVVILKKHGERATNVLSVFLCLFSALVFAFIGIGAFHSDS
                     FRGLFGGSWAIGAPTDGYLNITVALVLVIGLVLTRLSRRRISGGKIRYRYLLLLAALG
                     WLFFTPAPWIGPLFFVLTFLEYQTKRPLEIGFDQDHVVINTLIRQQFAWGDFNNVLLR
                     DGLLTLDFKSNRLIQKEVADDDEDDDADEEEFNAYCRTRLAAVAGN"
     gene            1916..2518
                     /locus_tag="VKQ52_10960"
     CDS             1916..2518
                     /locus_tag="VKQ52_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87756.1"
                     /translation="MDSISLPVVDPAFEKLKRNGVWLHTLAGALLLAHAISHFRSEST
                     HTLYFWCLLIISLDIFLLVLAGRHLLQQLPRVNLFFRFIEIIFFLCIGVLMIIEGNEL
                     TGIVHFLLSIAYGYLFYCERALRQTETLSFHHTGITIPGLPESRFLYWTHINNVNAAY
                     DSIDISTSEEEDLRFDLRVNLNYDQLETIQAFCRHYLGPR"
     gene            2567..>3303
                     /locus_tag="VKQ52_10965"
     CDS             2567..>3303
                     /locus_tag="VKQ52_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87757.1"
                     /translation="MLSESPFILYSDGKGNIFEDTSLYVTGRSGWDALPVPAEEWIPL
                     PDGGSLYELPGRRGIGIDVLTGEMRLCDKGWAVAAFIPPAHTGLYLAAYETGPDAPTL
                     PLFCYTAAGWHDGKFYVPAVRIEQDIRQDCAGYDWDIINDGVDRILKAYPQNRLVKHL
                     ADNCCLTYHCPAARNFFMGRWECPIPTSPACNANCIGCISFQPQEEAIPSTQDRLTFK
                     PLPEEIVEFTVPHLQEAPFPIVSFGQG"
BASE COUNT          712 a         1107 c          755 g          729 t
ORIGIN      
        1 ccatccactc cctccccatt tccccgcagc ccctgcggca acccgcatcg cttcagcgtt
       61 ccaacactaa ttttatcccc tttttttgcc atgtgtgtat actacgctaa cttgcgcacc
      121 gttttcgagg agcagcccct aaacaagaag cggcgctccg attttcagcc atgagactac
      181 tacgccatga cagatttatc gcattttgat tccaacggcc ccagcaaccc taagaataac
      241 atcttcggct taccgttctc agaagatgac gcccgtctcg tggtcctccc cgtcccctgg
      301 gaagtgaccg tcagctacgg cgccggcacc tcgagagcct gcgaacacgt cttccacgcc
      361 agcatgcagg tcgacctctt cgacggcgaa acaggcgacg cctggcgcca gggcctctat
      421 atgcggcaac ccgataaaaa ggtcctcatg aagagcgact atctccgcaa agaagccgaa
      481 ctctatatca acttcacctc caaggacgaa cccgtcgaga agaacagctt catgtgcaaa
      541 tccctcaaag agatcaacga aggaagcgcc agcctcaaca aatgggtcta cgaacaaaca
      601 aaaggactcc tcgaccaggg caaactcgtc gccctcctgg gcggcgacca tagcactccc
      661 ctggggtact tcaaggccat cgccgaaaaa catggcgact tcggcatcct ccaaatcgat
      721 gctcactgcg atctgcggaa gtgctacgaa gattttgttt actcccacgc cagcgtgatg
      781 tacaacgctt tacaggaaat acctcaacta aaaagattga tacaggttgg cgtccgcgat
      841 tactctctcg acgaatggga attcatccag agcagcaact ataaggtcat cacctatttc
      901 gataaggata tcaaagaacg cgggtatgaa ggccagacct ggcgacagat cgccgacgag
      961 atcgtgagcc atcttcccga gaaggtctat atcagcttcg atatcgacgg cctcgatccc
     1021 aagctctgcc cgcacaccgg cacccccgtc cagggcggct tcgagatcga acaaatattt
     1081 tatctgatcc gcaagatcat ccaaagcggt cgccagttcc tcgggttcga cctcgtcgag
     1141 atcggggtcg gcgacaccga ctgggacgcc aacgtcggcg cccgcatctt atggaaattg
     1201 agtaacttgc tggtggcaag caactcctga tcattgccct ctctttttat gcgctaccag
     1261 tatgtggtca tcctcaaaaa acacggcgaa cgggcaacaa acgtcctcag cgtttttctc
     1321 tgtcttttct ctgcccttgt ctttgcattc atcggtatcg gggcatttca cagcgactcc
     1381 tttcgggggc tcttcggcgg aagctgggcc atcggcgcgc ccaccgatgg ctatctgaat
     1441 atcacggttg cgctcgtcct ggtgatcggc ctcgtcctca cccgtctatc ccgccgacgc
     1501 atctccggcg gcaagatccg ctaccgctac ctgctgctcc tcgctgccct gggctggctg
     1561 ttcttcaccc ccgccccctg gatcggcccc ctcttcttcg tcctcacctt cctcgaatac
     1621 cagaccaaac gccccctcga gatcggcttc gaccaggacc acgtcgtgat caacaccctg
     1681 atccgccagc aattcgcctg gggagacttc aataacgtcc tcctgaggga cggtttactc
     1741 accctcgatt tcaagagtaa tcgattgata cagaaggagg ttgcggacga tgacgaagat
     1801 gatgatgcag atgaggagga gttcaacgcc tactgccgca cccgcctcgc ggccgtagcc
     1861 ggaaattaat tttatggaaa atgcgtatcc ccgcatcccc tgaaaaacta ctcttatgga
     1921 ttccatttcc ctccctgtcg tcgaccctgc tttcgaaaag ttgaagagaa acggcgtctg
     1981 gttgcatacc ctcgccggcg ccctgctcct ggcccacgcg atcagccatt tccgcagcga
     2041 atcgactcac acgctctatt tttggtgcct gctgatcatt tccctcgaca tcttcctgct
     2101 ggtgttagct ggccgccatc tgctccagca actcccccgc gtcaacctct tcttccgctt
     2161 catcgagatc atcttcttcc tttgcatcgg cgtgctcatg atcatcgaag gaaatgagct
     2221 caccggcatc gtccatttcc ttctttccat cgcttatggt tatcttttct attgcgaacg
     2281 ggccctccgc caaaccgaaa ccctctcctt ccaccatacc ggtatcacca tccccggcct
     2341 gcccgaaagc cgcttcctct actggaccca tatcaacaac gtaaacgctg cctacgacag
     2401 catcgatatc tccacctccg aagaagaaga cctgcggttc gacctgagag tcaatctcaa
     2461 ctacgatcag ctcgagacca tccaggcctt ctgccggcat tatctgggcc cgcgataaac
     2521 catcggtttc caccacacca ttgccgaaca ttccctactt ttgcggatgc tttcggaatc
     2581 accgttcatc ctctattccg acggaaaggg gaatattttt gaagacacct cgctttatgt
     2641 caccggccgc agcggctggg acgccctccc cgtacccgcc gaagaatgga tccccctccc
     2701 cgatggcggg tccctgtatg agctgcccgg ccgccggggc atcggcatcg acgtcctcac
     2761 cggcgagatg cggctttgcg acaagggctg ggccgtggcc gcctttattc cccccgctca
     2821 caccggcctc tatcttgcag catacgaaac aggccccgac gccccgacac tccccctctt
     2881 ttgctatacc gccgcaggct ggcacgacgg gaaattttat gtccccgccg tgcgcatcga
     2941 gcaggatatc cgccaggact gtgcgggtta tgactgggat atcatcaacg acggtgtcga
     3001 tcgcatcctg aaagcgtatc ctcagaaccg tctcgtaaaa cacctcgcgg acaactgctg
     3061 cctcacctat cattgccccg ccgcacgcaa tttctttatg ggccgctggg aatgccccat
     3121 ccccacctcc ccggcctgca acgctaattg catcggctgc atctccttcc aaccacagga
     3181 agaggccatt ccgtcgacgc aggaccgcct cacgttcaaa cccctgcccg aagagatcgt
     3241 cgagttcacc gttccgcacc tgcaggaggc gcccttcccc atcgtgagct ttggccaggg
     3301 ctg
//
LOCUS       DATGFG010000587         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_913393, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000587 DATGFG010000000
VERSION     DATGFG010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913393"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VKQ52_10970"
     CDS             <1..1000
                     /locus_tag="VKQ52_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ87758.1"
                     /translation="AGLVMINITPDIRKNYTIIRDDLKRSKAIVEMGASQNSPTEYNS
                     VDNRFNWKGKDPGMNADFAMSNMTLEYGKTIGWHILEGRDFSPDFPSDSTAFIINEAA
                     AKQMGFKHPIGETVIWNMKPFHVIGMIRNIVFESPYSISVSPSIFHISGGDNYVTTLR
                     LNPHLSMQEALARTRTVFTQYNPTYTFDYRFVDKEYAKKFNEEERMGQLSGFFTLLAI
                     FISCLGLFGMASFMAEQRRKEIGVRKALGASVFNVWRLLSREYVILVIISLVIATPIT
                     FYFMHNWLQQYEYRYKMSWIVFTEAGIGALLIALLTISYQVLKAAVASPVRSLRTE"
     gene            1100..1582
                     /locus_tag="VKQ52_10975"
     CDS             1100..1582
                     /locus_tag="VKQ52_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87759.1"
                     /translation="MSIDNSVSLMTRLAELKHSTTLFLESIRGEYLKVLLESQAEVWA
                     HSNTIIERVTRLYFDSSDLPVLYCTSHLNKSNLTVQEYTSLAEGLLPLGIVFHRYNHA
                     HSIKKTNVVLSEEINRDIARSLNVTSPLIFQKRYDYWVGDRQIGYICEFFNEESLDRV
                     "
     gene            1579..>2798
                     /locus_tag="VKQ52_10980"
     CDS             1579..>2798
                     /locus_tag="VKQ52_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLZ87760.1"
                     /translation="MNYQPFTADAGHLSGENILRDDTLTVPYSGIGELLEQLDRFFSD
                     HDKKKICCVALITENTVAHALIILYLLREKLNFILLNGHAPVEDKIPFFCDIILTVAP
                     SVSGRHDWPGNLQLVHNPGHKRNRFAITRHSGSIFFLSSGTSGQAKYIYFKQQNLLRN
                     ANNCVKRFAIDDRSRVLVPVPVNHMYGMGVGLLPALLSGASICLIDKNNIVKLLDRIS
                     TFQPSVTLFTPAVIKMLLLLRNSVQKKGVYITAGEKLADQAYKDFEYRYGPLFNLYGC
                     TELGAIAVSDADGNLKALEQVKVRISPAENGQIVCRHNAGFVSYLDRHGHRVPSQKEG
                     QGWYRTNDIGMVTGDQGFRVIGRADHCTNRYGFLVSLDEVASLLESLFAEINQAIVFE
                     GNKEKGLSTNLVAV"
BASE COUNT          711 a          728 c          680 g          679 t
ORIGIN      
        1 tgccggcctg gtcatgatca atataacacc ggatattcgg aagaattaca cgatcatccg
       61 ggatgacttg aaaagatcga aggccatcgt ggagatggga gcatcgcaaa acagcccgac
      121 agaatataat tccgtggata accgtttcaa ctggaaagga aaagacccgg gtatgaatgc
      181 cgattttgct atgagtaata tgaccctgga atatggaaag actatcggct ggcatatcct
      241 ggaaggccgt gacttctccc ccgatttccc atccgattcc acggctttta tcatcaacga
      301 agcagcggca aagcagatgg gtttcaagca tcccatcggc gaaaccgtta tctggaatat
      361 gaagcccttt catgtgatcg ggatgatcag gaatattgtg ttcgagtctc cctattccat
      421 ttcggtgagc ccgtccattt tccatatttc cggcggtgat aactatgtga ccacgctcag
      481 gctcaacccc cacctgagca tgcaggaagc cctggcacgg acgagaacgg tcttcaccca
      541 atataacccc acctatactt tcgattaccg gtttgtggat aaggagtatg ccaaaaaatt
      601 caacgaagag gaaagaatgg gccaattgtc gggttttttc accctgctgg ccatatttat
      661 aagctgcctc ggtcttttcg gtatggcttc ctttatggcc gaacagcgca ggaaagagat
      721 cggtgtacgc aaagcattgg gagcatccgt attcaacgtc tggcggctgc tgtcaaggga
      781 atatgtgata ctcgtcatta tctccctggt tattgcgacg ccgataacgt tttacttcat
      841 gcataactgg cttcaacaat acgaataccg ctataagatg tcctggatcg tctttacgga
      901 ggccggcatc ggcgccctgc tgatcgccct tttgaccatt agctaccagg tgttgaaagc
      961 ggcggtcgcc agcccggtca ggagcctgcg gacagaataa tccctgccct acccaattat
     1021 tttcctcttt taccgataac gttacccggg cagcctgaat gttcgggtta ccgcctttat
     1081 ttaatctaat ccttccgaca tgtcgatcga caatagtgtc agcctgatga ccagactcgc
     1141 agagctaaag cattccacga ctttattcct cgaatccatc aggggtgaat acctgaaggt
     1201 cctgctggaa tcacaagccg aagtatgggc tcactcgaat actataatcg aacgggtgac
     1261 aaggctgtat ttcgattcat cggatctccc ggtactctat tgtacaagtc atttgaataa
     1321 aagcaacctg accgtccagg aatatacttc gctggcggaa ggcctgttac ctctcggcat
     1381 tgtattccat cgctacaacc atgcccattc gataaaaaaa acaaatgtcg tcctgagcga
     1441 ggagatcaac agggatattg ccagatccct gaatgtgacc tccccgctga ttttccaaaa
     1501 aagatatgat tactgggtcg gcgaccgcca gatcggctat atctgcgaat ttttcaatga
     1561 agaatccctg gacagggtat gaattaccag ccttttaccg ccgatgccgg tcatctttcg
     1621 ggggaaaata tcctgcggga tgacacgctc accgtaccct atagcggcat cggcgagcta
     1681 ttggagcagc tggatcgttt cttctccgac catgataaga aaaagatctg ttgtgtcgcc
     1741 ctgatcaccg aaaatacggt tgcccacgca ctgatcattt tatacctgct tagagaaaag
     1801 ctgaatttca tcctgctgaa cgggcatgct cccgtcgagg ataagatccc cttcttttgc
     1861 gacatcatcc tgaccgttgc cccctctgta tccggccgcc atgactggcc tggcaacctc
     1921 caattggtcc acaaccctgg tcacaagaga aacaggttcg ccataacacg acattcaggc
     1981 tccatatttt tcctgtcgtc aggcacgtcc ggccaggcga aatacatcta cttcaaacaa
     2041 caaaacctcc tccgaaacgc caacaactgt gtgaagaggt ttgccatcga tgaccgcagc
     2101 agggtcctgg tccccgtccc tgtcaatcat atgtacggca tgggagtcgg cttactgccg
     2161 gcgctgcttt caggcgccag catttgtctc atcgataaga ataatatcgt caagcttttg
     2221 gacaggatat ccaccttcca acccagcgtc acgttattca caccggcggt gataaagatg
     2281 ctcctgttac tcaggaacag cgtccaaaag aaaggcgtct acatcactgc cggtgaaaag
     2341 ctggccgatc aggcatacaa agactttgaa taccggtatg gcccgctctt caatctttat
     2401 gggtgcacgg agttgggcgc cattgccgtc tccgacgccg atgggaatct gaaagcattg
     2461 gaacaggtga aggtcaggat cagccctgcg gagaacgggc aaatagtatg caggcacaat
     2521 gcgggcttcg tctcttacct tgaccggcat ggtcatcgcg tgccttccca aaaggagggc
     2581 cagggttggt acaggaccaa tgatatcggc atggtcacgg gagaccaggg ctttcgggta
     2641 ataggacgag cggatcactg cacaaaccgg tatgggttcc tggtatcgtt ggatgaggtt
     2701 gcctcccttc ttgaaagcct ttttgccgaa ataaaccagg cgatcgtctt cgaaggaaat
     2761 aaggaaaagg ggctgtcgac gaacctggta gccgtgtg
//
LOCUS       DATGFG010000588         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_924468, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000588 DATGFG010000000
VERSION     DATGFG010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924468"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VKQ52_10985"
     CDS             <1..738
                     /locus_tag="VKQ52_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ87761.1"
                     /translation="QVAKTGDGQLAYNPSSANSSSPSNEKPQGLGFNILSTPRAGQFS
                     LALPDGSKVWLNNASSLRYPVAFTGATREVELSGEAYFEVAKNATHPFYVHTTGGPAG
                     PATIEVLGTSFNIMAYGDEASERATLVDGSIRYAHGASSTLLKPEEQSVLDAHGNLQT
                     LRHVNIAEITAWKSGYFHFDHANLETTMRQLARWYDVSVEYAGPIPPQEFVGKIQRSM
                     PLSAVLKGLEGEHVHFQLEGRKLVVKP"
     gene            complement(758..>2306)
                     /locus_tag="VKQ52_10990"
     CDS             complement(758..>2306)
                     /locus_tag="VKQ52_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87762.1"
                     /translation="RRLAGQDTTLYPGGVQEEETHQGRASTSMETLGRYSGELGPGRL
                     EAVVGLSYQQQRTSYHRLLASDYPSDALLSAGTGATALPDQQNSFSYRYLTFFQRSNY
                     NLSRKYSLSAAIRREDIGGPGMAMPAGYFWNAGAAWVFSREAFLADSRLLSFGKIKVT
                     YGTTGNDPGAGWQDPIGMPPPNARLPWQRNVNEELGLDLGFLHDRLQLSGMFLRGWSG
                     NQLLTVTPDNGPVMPLMAVDHSGLVVVDHPGIKVENRVFEMELSGRRMRLGPLQWSST
                     LNLTIPRNRLAQWPGLAGSAYSGEYVAGKSLSVSSAYHYLGVDSRTGLYSFQTKNPDG
                     LPGPGDRVPDAGFDPNYYAGWNNDFRIGNWQLEFFVLFRSQRGVNPLIALARQNAPGM
                     QAAGQLSNGPVEWLTHWRKPGDVASQQRLTSGQDAAALARLNDYLASDGAVTDASYVR
                     VKRIVLGWNMPEKKAKKMKMQTCRVFLDGANLWTITHFPVTDPETQDPRVLPPMRELS
                     VGVKVGF"
BASE COUNT          489 a          762 c          609 g          446 t
ORIGIN      
        1 caggtggcca aaacgggcga cggtcaatta gcatacaacc cgagctccgc caattcctct
       61 tccccttcga acgaaaaacc ccagggcctc ggcttcaata tcctgagtac tccccgtgcg
      121 gggcagttct ccctcgctct ccccgatggc agcaaggtct ggctcaacaa tgcatcctcg
      181 ctgcggtatc cagtcgcctt tacgggagct acccgcgaag tggagcttag cggcgaagcc
      241 tatttcgaag tggcgaaaaa cgcgacgcat cccttttatg tacataccac cggcggcccc
      301 gcaggccccg ccaccatcga agtcctcggc acatccttca atatcatggc ctacggcgat
      361 gaggccagcg aacgcgcgac cctggtcgac ggcagcatcc ggtatgcaca tggcgccagc
      421 agcaccctgc tgaaacccga agagcaatcg gtgctcgacg cccacggcaa cctgcaaacg
      481 ctgcgccatg tgaacatcgc cgagatcacc gcctggaaga gcggctactt ccacttcgac
      541 cacgccaatc tcgagaccac catgcggcaa ctggcccgct ggtacgatgt ctccgtcgaa
      601 tatgccggcc ccattccgcc ccaggaattt gtcgggaaga tccaacgcag catgcccctc
      661 tccgccgtgt tgaaaggcct cgaaggcgaa cacgtgcatt ttcagctgga aggaaggaag
      721 ctggtggtga aaccctgatc agcgtctccc ttattttcta aaatcccacc ttcaccccca
      781 ccgacagttc ccgcatcggc ggtagcaccc ttgggtcctg cgtctccggg tcggtaacgg
      841 ggaaatgtgt gatcgtccac aaattcgccc catccaggaa cacgcggcag gtttgcatct
      901 tcatcttctt cgccttcttc tccggcatat tccagcccag tacgattctc ttcacccgca
      961 cgtaggaggc atcggtaacc gcgccatccg acgcgagata gtcgttcagc cgggccaggg
     1021 ccgcggcatc ctgcccagac gtaagccgtt gctgcgaagc gacatccccg ggctttcgcc
     1081 agtgggtcag ccattcgacg gggccgttgc tgagctgtcc cgccgcctgc atcccgggtg
     1141 cattttgccg ggcaagggcg atcaatgggt ttaccccccg ctgacttcga aagaggacaa
     1201 agaattccag ttgccagttc cctattcgga aatcgttgtt ccaaccggca taatagttcg
     1261 gatcgaagcc cgcatccggc accctgtcgc ccggtccagg cagcccatcg gggttcttcg
     1321 tctgaaaact atacagcccg gtcctgctgt cgacacccag gtaatgatag gcggaagaaa
     1381 cagaaaggga tttgccggca acatattccc cggaataagc ggaacccgca agacccggcc
     1441 attgcgccag ccggttgcgg ggtatcgtca ggtttagggt cgagctccat tgcaaagggc
     1501 caagccgcat tctcctgccc gacaattcca tctcgaaaac ccggttctcc acttttatcc
     1561 cgggatgatc gaccaccact aaaccggaat gatcgactgc catcagcggc ataaccgggc
     1621 cattgtccgg ggtcacggtc agtaattggt tgcccgacca accccggagg aacatcccgg
     1681 acaattgcag ccggtcatgc aggaaaccga ggtccaatcc cagctcttca tttacattcc
     1741 gctgccaggg caatcgtgcg ttgggcggcg gcattcctat tgggtcctgc caacccgcgc
     1801 cggggtcatt accggtggtg ccgtaagtga cctttatttt tccaaagctg agaagccggc
     1861 tatccgccag gaaagcctcc cggctgaaga cccatgcggc gccagcattc cagaaataac
     1921 ccgccggcat cgccataccg gggccaccga tatcctccct ccggatagca gccgagagac
     1981 tatattttcg cgaaagatta tagttcgacc gttggaaaaa ggtaaggtac cgataggaaa
     2041 agctgttctg ttgatccggc aaagccgtcg caccggtgcc ggcactcagc agggcatcgc
     2101 tgggatagtc actcgccagc aaccggtgat agctcgtgcg ctgctgctgg tagctcaggc
     2161 ccaccaccgc ctccaatcgg cccggcccca gttcacccga ataacggccc agagtttcca
     2221 tcgatgtaga agcccgcccc tggtgggtct cctcctcctg tactccccca gggtaaaggg
     2281 tcgtatcctg ccccgccagc cggcgg
//
LOCUS       DATGFG010000589         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1005698, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000589 DATGFG010000000
VERSION     DATGFG010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005698"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..619
                     /locus_tag="VKQ52_10995"
     CDS             215..619
                     /locus_tag="VKQ52_10995"
                     /inference="COORDINATES: protein motif:HMM:NF025808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiubiquitin domain-containing protein"
                     /protein_id="HLZ87763.1"
                     /translation="MEQQEHRPERPKEEEIKELREELARDQEVQEQAEKIVQETDADM
                     RRIEKKLEELEHPEIELVVNGEPKLWHQREISYKEVVILYYGTYEDDEAITYSVDYSH
                     GPEGHREGILKKGQSVPVINKMRFRVQRTNRS"
     gene            594..1793
                     /locus_tag="VKQ52_11000"
     CDS             594..1793
                     /locus_tag="VKQ52_11000"
                     /inference="COORDINATES: protein motif:HMM:NF004804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HLZ87764.1"
                     /translation="MSRELIARSPDLARLQEEGYEIRFREGFLLTSSVPYLDCQLKLR
                     YGTLACEVTAEKGVAVRPAAHPMWWQGTYPFQTDGVAIMAIQNPTQGKTLLTGFTVDF
                     LFSARPVDRDGTFIDYPNFYEKVKTYIGIISGPALQLYPDAVLKSERVFAEEEDDSPF
                     LYPDTNTSRAEINAISDKVKGQKIGIIGVGGTGSYILDLVSKCLVAEIHLFDADPFAQ
                     HNAFRSPGAASEADIDAKCKKVAYFQRIYSKMRRGVIAHEVRLTAAQFPLLDALTFVF
                     IAIDKTGIKRELFDYLVTHNIPFIDVGIGVTRREDTLLATARVTAADAQRHSHMPEHV
                     SMEPDKDEQDDYGTNIQIAELNALNAAVAVIHWKRKYGVYDNTDKSMHIQVAIEQIKI
                     YTDEDAP"
     gene            2289..2681
                     /locus_tag="VKQ52_11005"
     CDS             2289..2681
                     /locus_tag="VKQ52_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLZ87765.1"
                     /translation="MANFKTHLVTGALAGGCILLGCNLMDQSNRIEKGQMPAYDGGEM
                     LKQLLVGLAIGGLAGILPDILEPADSPFHRKFWHSGLLGVALCHGMVKLGQSGLSEQT
                     KVCLQGGTAAYLSHLLLDLSTPMGLPLV"
     gene            complement(3045..3119)
                     /locus_tag="VKQ52_11010"
     tRNA            complement(3045..3119)
                     /locus_tag="VKQ52_11010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3082..3084),aa:Val,seq:tac)
     gene            complement(3291..>3789)
                     /locus_tag="VKQ52_11015"
     CDS             complement(3291..>3789)
                     /locus_tag="VKQ52_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87766.1"
                     /translation="FFKVMDNEAGEDLSWFWRGWFLHNWSYDIAVQSVQNSAPATASA
                     TPNSASGSAPNSAGSTTNNSASGTNSNGSSNSTAIATPNSAASAEVAITLANLDRMAF
                     PAILELTWKDGTKQRISLPVETWLQSGTRTLRFPATQPLAAVTVDPDHALPDANRANN
                     TWTAP"
BASE COUNT          975 a          788 c         1108 g          918 t
ORIGIN      
        1 cagcattgac gtagttccct cctacgagct gtatgaatac attttaggaa agatgccgga
       61 tgtaaaggta atctcgccgc cggcggtcgt aaagcacatt ctgaaagaga taaaggctgg
      121 cctcaaaaaa aatagttgaa aaagttcgga ttttttcgat ggcgaacgat cagttcgcca
      181 tccggtgttt tatttgtgta taagaaacaa aaatatggaa caacaagaac accggccaga
      241 aaggcccaaa gaggaggaga tcaaggaatt gcgggaggaa ctggcccgtg atcaggaagt
      301 tcaggagcag gcagaaaaaa ttgtccagga gacggatgca gacatgcgcc gcatcgaaaa
      361 gaaattggag gaattggagc atcctgagat agaactagtc gtcaacggtg agcctaagct
      421 ctggcatcaa cgagagattt cctacaaaga ggtcgtcatt ctctattatg gtacatatga
      481 ggacgacgag gcgatcacct attcagtcga ttattctcat ggacctgaag gtcatcggga
      541 ggggatactg aaaaagggac aatccgttcc ggtcattaac aaaatgcgtt ttcgtgtcca
      601 gagaactaat cgctcgtagc cccgacctag ccaggctcca ggaagagggg tatgagatcc
      661 ggttccgtga gggtttcctt ctgaccagca gtgtgccgta tctcgattgc caattgaagt
      721 tgagatatgg gacactcgct tgtgaagtga ccgctgaaaa gggggtggca gtgcgtccag
      781 ctgctcatcc aatgtggtgg cagggcacat accctttcca gacagacggg gtggcgatca
      841 tggcgatcca gaatcctacg cagggaaaaa ctctgctcac agggttcacg gtcgactttc
      901 tcttctctgc tcggcccgtt gatagggatg gaacgtttat agactatccg aatttttacg
      961 aaaaggtaaa gacctacatt ggcattatct cgggtccggc cttgcagctc tacccagatg
     1021 ctgttttgaa gtctgaacgg gtttttgcgg aggaggaaga cgacagcccg ttcctctatc
     1081 cagacacgaa cacttctcgg gccgagatca atgcaatctc tgacaaagta aagggacaga
     1141 aaatagggat tataggtgtt ggcggtaccg gatcatacat cctggacttg gtgtcgaaat
     1201 gcttagtagc ggaaatccac cttttcgatg ctgatccatt cgcccaacac aatgcgtttc
     1261 gatcacccgg ggcggcctca gaagcggata ttgatgcaaa atgtaagaag gtcgcctatt
     1321 ttcagaggat ctactcgaag atgcgtcggg gcgtcattgc gcacgaggtc cggctgaccg
     1381 cggcacagtt tcctctgttg gacgccctta ccttcgtttt cattgcgata gataagacgg
     1441 ggattaaaag ggagctattt gactacctgg taacgcacaa tataccgttc attgacgttg
     1501 ggatcggtgt gaccaggagg gaagataccc tcctggccac ggcacgggtg accgctgccg
     1561 acgcccaacg acattcgcac atgcccgagc acgtttctat ggaaccagat aaagacgaac
     1621 aggatgatta cggaaccaat atacagattg cagagcttaa cgcactgaac gccgcggtag
     1681 cggtgatcca ctggaaacga aaatatggtg tttatgacaa cactgataaa tcgatgcata
     1741 ttcaagtggc tatagaacaa attaagatat atacggatga agatgcgcca taaattcgta
     1801 gagttccttc cttcggagaa agagccgggt acgctatata tttcagtcga gtttggcgtc
     1861 gcggtgcatc tctgcgcctg cggatgtggg caggtggttg tgacgtcact cgcgccgtac
     1921 gcgtgggcat tcttatatga cggagaaacc gtctccttgt acccatcgat tgggaactat
     1981 tcactgccat gcaggtctca ttactggata agatggggag aggtgttgtg ggccaataaa
     2041 ctgacaaata aagaaatcga tcgggtccgt cgccgggatg tgttggcgct tgaacaaaag
     2101 gacaaaggac agaaaaagag gacgggaaat agtcgtttga aagaatagac tcaccgatct
     2161 gttaagcgcc cttaagcagg gtttgagaaa aagttctgga aaatttaact attctgactg
     2221 atcaggaacg atcagctcgc gtacacatct tttcttttta tccaatgaca ttataaaaat
     2281 ttaatccgat ggcgaatttc aaaacgcact tagttactgg agctctagcg ggcggctgta
     2341 ttttgctggg ctgcaatctt atggatcaat caaatcgaat cgaaaagggg cagatgccag
     2401 cctatgacgg cggggaaatg ctgaagcaat tattggtagg gttggcgatc ggcggtctgg
     2461 ccggcatcct gccggatatt ctggagcctg cggacagtcc atttcaccga aaattctggc
     2521 atagcggctt gcttggagtg gcactttgtc acggcatggt gaagctcgga cagtcaggtt
     2581 tatcggaaca aactaaagtc tgcctacagg gtggcacggc tgcatatctg tcgcacttgc
     2641 ttttagattt gagtactccg atggggctgc cgttagtgta ggctggagat aaaaattgga
     2701 tcaactggga gctgtctgtt tttcttgtaa atggtgtttt atgcaacgat ataaaaattt
     2761 aagcggcgat tcgggggtgt atgcgtttga aattggcatg gatcatatta gagtgcagtt
     2821 ccacggaacc ggcaaaattt atcggtatag ttattataga gctggcatga cgcatgtcga
     2881 ggaattaaaa cgtcttgcac tgattgggcg aggtctgaat agttatatcg taaggaacgt
     2941 tagattcctt tatgattaaa cgcaatggta gaagcgcgaa ggtgccggtg ttaggcacaa
     3001 tgttggtaca aataaaaaaa gcacctttta agtgcttcat ttcgtgggag ttgacgggtt
     3061 cgaaccgccg accctctgct tgtaaggcag atgctctgaa ccagctgagc taaactccct
     3121 tttcctttaa acggttggtt ttccgttgaa aggagtgcaa agataaggct taaaagatat
     3181 ctgcaaaaaa ttttttgacc atttttgggg tgaatttttg gtgcaactcc atgagggaga
     3241 tggatatatc attttgaaat ttaccaggtt cggggtatcg acaggccggc tcagggcgcg
     3301 gtccacgtgt tgttggcgcg gttggcgtcg gggagcgcgt ggtcggggtc gacggtgacg
     3361 gcagccaggg gctgggtggc gggaaagcgg agggtgcggg tgccggattg gagccaggtt
     3421 tcaacgggga gggatatgcg ctgtttggtg ccgtccttcc aggtgagctc gaggatggcg
     3481 gggaaggcca tgcggtcgag gttggcgagg gttatggcga cctcggcgct ggcggcggag
     3541 ttgggggtgg caatggcggt ggagttggag cttccattgg agttggtacc ggaggcggag
     3601 ttattggtgg tgctgccggc ggagttgggg gccgaaccag aggcggagtt gggggtggca
     3661 ctggcggtgg cgggtgcgct gttctggacg gattggacag cgatatcgta actccagttg
     3721 tggaggaacc agccgcgcca gaaccaggaa agatcttcac ccgcctcgtt gtccatcact
     3781 ttaaagaaa
//
LOCUS       DATGFG010000590         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_480166, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000590 DATGFG010000000
VERSION     DATGFG010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480166"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(771..1244)
                     /locus_tag="VKQ52_11020"
     CDS             complement(771..1244)
                     /locus_tag="VKQ52_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87767.1"
                     /translation="MTFATTTSQETQAGILKSKIVELQSALFFTESRSIVTLPTHVIS
                     DVELDNEGQIWFVIPKPAMQIEAYDKEIPAKLDFFKKGKDFFVKVRGTAFLQTELTEA
                     NENAGLSAEMKARMNDAAVIAVKIKIQDFELVDNSPKPTQSWLQASRSQLSSWFF"
     gene            1524..2693
                     /locus_tag="VKQ52_11025"
     CDS             1524..2693
                     /locus_tag="VKQ52_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLZ87768.1"
                     /translation="MRACLFAFLLLSLSVSTLGQQRERFPVDTVPSKKDLVPFTIKLP
                     QLPRRDTTTAPSPNKNLLAFPFAVRSLETNWGFGGIAARFFKAQQHDTTIRTSDVNIL
                     GLYTVRSQLILVLSSTIFFPKEDHIARFQASYSYYPDDFWGLGNHTSFTDKEGFSQKQ
                     YFVNPQFLKRVHGNMYLGITYEFQHTGPVTYTPGGVFDKENIAGRHGGNTSGIGPILS
                     WDTRNNAYSPDHGGFAEIQFEYFDKYLGSDFNFRLLSVDLRKFVHLSDKEVLALQGLG
                     GFTFGNTPFRKLEELGGADMMRGYYGGRYTDKCLMAYQAEYRRFLFWRIGMVGFIATG
                     EVAPTPGRFELDGFHSAYGGGLRFALSKEEKLNLRVDYGVSKHANAFTVQLREAF"
     gene            complement(2748..3113)
                     /locus_tag="VKQ52_11030"
     CDS             complement(2748..3113)
                     /locus_tag="VKQ52_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HLZ87769.1"
                     /translation="MAWIYLFVASVFEIAWTFSLRFMSVKKLRALPWKGFFSQWENWA
                     VLAPFAGYVLFGLGNIYFFSLAMKEIPASTALAVWMGTALIGVKFVEIVFLRTPYDWQ
                     QFIYMGFILVGIVGLKRMP"
     gene            complement(3282..4250)
                     /locus_tag="VKQ52_11035"
     CDS             complement(3282..4250)
                     /locus_tag="VKQ52_11035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HLZ87770.1"
                     /translation="MGTIVYYTTGILILSVFVFIWLERKFPYTKGIPVFRDGFWVDLI
                     WYTFIQSYFLKIFIFGYLIEPMRRNWHLQSMHLLDGWPVALQVLLFLVIHDFYIYWFH
                     RAQHHSKLLWRTHEAHHSNKEVDWLAGTRSHVVEIIINQTIEFAPIVLLLGPDSPVVA
                     IKALIDAVWGMYIHSNIDVKSGKLQYFINGPEMHQWHHANDRAVFYANYSTKFAIWDW
                     IFGTAYLPGRKKPSEYGLYYDYPKDYFLQHWFSVRRMDEKKLVDRHPWFRAYYFSRPR
                     LWQWMLRKLRLTAGQEERQAGAPETERGAAPELKAAASHGEGPARA"
BASE COUNT         1059 a         1275 c          939 g         1000 t
ORIGIN      
        1 tctcttcgcc gcagttcttt actaactacc cttcctcaca ccacccgccc tcccggggca
       61 gaaaaaaatc atccatattt tgccaaatta tttagcaaaa acacctatct ttaccacctg
      121 cggtcctgac aggcggggtt gcaccgatca agccatagtc attacgcctt atccatcaga
      181 agccgccgct tgtggctttg tccgaagatt ggccggctgc cgcaaggtag ccggtcagtc
      241 gcattctacc cggaaaccag tatgaaacga cacaggacag tacaggatgg tgatgcgtgg
      301 agctacccgt tttttcgttt gggcttcatg cgaaaaattc gctggaatgc agtaacggaa
      361 agggttttac ataaattttc acagctttga gataatagaa ttaacattaa gttgtttgcc
      421 ctgcaccgga aaagcatact atatttgtat catcaaacac gaaatcatca gggcgccgat
      481 gatcaccaac agagaacggc gccaatcgag ataaagagtt ttcatatggc aagcaatcgt
      541 tagaggtcga ctgtttctac agtggacctt cttttttttc cctacttttt cgctcgcgcc
      601 tccacatcct ctcttcgcat cctctcttcc tctccccgcg cgatccccct gcactccgaa
      661 cttcgctcgg atatccccaa aaactgaaat ccgcgaacta tgtcgcggac tcagcttcct
      721 atatgtaaca cgcgcagtat gcaaaacgat tcggttcccg ggatactcgc ttagaagaac
      781 cagcttgaca actggctccg gctggcctgc agccaactct gagtaggttt ggggctatta
      841 tccaccagtt cgaaatcctg tatcttgatc ttgacggcaa tcaccgctgc atcattcatc
      901 ctcgccttca tctccgcaga cagacccgca ttttcatttg cttcggtcaa ttcggtctga
      961 aggaatgccg tacccctcac cttgacaaag aagtccttcc ctttcttaaa gaaatccagc
     1021 ttggcaggga tctccttatc ataagcctcg atctgcatcg ccggcttcgg gatcacgaac
     1081 catatctgac cttcgttatc cagctccaca tcggagatca catgggtcgg aagggtaacg
     1141 atcgaacggc tttcggtaaa gaacaaagca ctctgcaatt cgacgatctt actcttcaag
     1201 attccggctt gtgtttcctg ggaagtggtg gtagcgaatg tcataatttc aagtatttgg
     1261 gtagagatta agtgttgttt ttgtcttcgg agttttttcg tcatccgtag acatttaggt
     1321 aaagacaatt ggcataccaa ctctcaaaac actgatttca aactacttaa aaacccttag
     1381 acccgtgccg gccgtcccag tttgggatta actttccaaa atttgattgg agacccattt
     1441 taattgatct ttgcgccgct aaggtttgaa cctgtagcgc ccttttctgt taaacctaac
     1501 cgaaagacac cattatatta tatatgagag cctgtctctt tgctttttta ttgctatccc
     1561 tttccgtatc aacgctcgga caacagcggg aacgctttcc cgtcgatacc gtcccgagta
     1621 agaaggacct tgttcccttc accataaaac tcccccaact cccgcgcagg gacacgacca
     1681 ccgccccctc ccccaataag aacctgctgg ccttcccctt cgccgtccgc tccctcgaga
     1741 ccaactgggg ctttggcggc atcgcggccc gcttcttcaa ggcccagcaa cacgatacca
     1801 cgatccggac atcggatgtc aatatcctgg gcctgtatac tgtgcggtca caactgatcc
     1861 tcgtcctcag ctccaccatc ttctttccaa aagaagacca tatcgcccgc ttccaggcct
     1921 cctatagcta ttatcccgat gatttctggg gcctggggaa tcacacctcc tttaccgata
     1981 aggaagggtt ctcgcagaaa cagtattttg tcaatccgca attcctaaag cgggtgcacg
     2041 gcaatatgta cctggggatt acctatgaat tccagcacac cggcccggtg acctataccc
     2101 ccggcggcgt cttcgacaag gagaatatcg cgggcagaca tgggggtaac acctccggca
     2161 tcggccccat cctctcctgg gacacaagga ataatgccta tagcccggac cacggcggct
     2221 tcgccgagat ccagttcgaa tatttcgata aatacctggg gagcgatttc aacttccgcc
     2281 tcctgagcgt cgatctccga aaatttgtcc acctctcgga caaagaggtc ctcgccttac
     2341 agggactcgg cggcttcacc tttggcaata ccccgtttcg caaactcgaa gaattaggcg
     2401 gggccgatat gatgcgcggc tactacggcg gacgatatac ggacaaatgc ctcatggcct
     2461 accaggcgga ataccgccgc ttcctcttct ggcggatagg aatggtgggc tttatcgcca
     2521 ccggagaggt agcgccgacc ccaggccgct tcgaactcga tgggttccac tcggcctatg
     2581 gaggcggatt gcgttttgcc ctaagcaagg aggaaaagct caacctgcgg gtggattatg
     2641 gcgtctccaa acatgccaat gcctttaccg ttcaattaag ggaagctttc taaaaccagc
     2701 catcaaaggg cccgctggtc aggcctggcg cctccgggct ttgtcattta cggcatcctc
     2761 ttcagcccaa cgatcccgac caggataaaa cccatataaa taaactgctg ccagtcatac
     2821 ggagttcgga gaaatacgat ctccacaaac ttgaccccga tgagcgccgt ccccatccaa
     2881 acggcaagcg ccgtagacgc agggatctct ttcatcgcca gcgaaaagaa atagatattg
     2941 ccaagcccga acaacacata ccccgcaaag ggcgccaata cggcccagtt ctcccactga
     3001 ctaaaaaatc ccttccaggg cagggccctt agcttcttca cactcataaa ccgcagcgag
     3061 aaggtccagg cgatctcaaa aaccgacgct acaaaaagat atatccaggc cataagagaa
     3121 tttcggcgaa aaattggcga aatataattt ttcgcctcaa tatatacaat atctgtacac
     3181 ttcgtgtacg aaccggattc catccggttt tggtaaaatc ttaaattcat gccccgggcc
     3241 attacgcccc cgccggctcc cttacgcccc cgccggcccc attacgccct cgccggcccc
     3301 tcaccatgcg aggcagccgc cttcagttca ggcgccgccc ctctttccgt ctccggcgcc
     3361 ccggcctgcc gctcctcctg ccctgccgtc agccgcagct tccgcaacat ccactgccac
     3421 agccgcggcc gggaaaaata ataggccctg aaccagggat gccggtcgac cagcttcttc
     3481 tcatccattc gcctcaccga gaaccagtgc tgcaggaagt aatccttcgg atagtcataa
     3541 tataacccat attccgaagg cttcttccgc cccggcaggt atgccgtccc gaagatccag
     3601 tcccagatcg cgaatttagt agaataattg gcataaaaca ccgccctgtc attggcatgg
     3661 tgccattggt gcatctccgg accgttgatg aagtactgca atttcccact ctttacatcg
     3721 atattggaat ggatatacat cccccatacc gcgtcgataa gcgcctttat cgccaccacg
     3781 ggcgagtcgg gccccagcag caacacgatc ggcgcaaatt cgatcgtctg gttgatgatg
     3841 atctccacca catgcgaccg cgtacccgcc agccagtcca cctccttatt cgaatgatgc
     3901 gcctcgtgcg tccgccacaa cagcttggaa tgatgctgcg cccggtggaa ccagtagata
     3961 taaaaatcat ggatcaccag gaagagcagc acctgcagcg ccaccggcca gccatccagc
     4021 agatgcatgg attgcagatg ccagttccgc cgcatcggct cgataagata cccgaaaatg
     4081 aagatcttca ggaaatagct ctggataaaa gtataccaga taaggtcgac ccagaatcca
     4141 tcccggaaga cgggaatccc cttggtataa gggaatttcc gctccagcca gataaagaca
     4201 aagacgctca atatcaggat cccggtggta taataaacaa tagttcccat gttcttacaa
     4261 aggtcttaca atg
//
LOCUS       DATGFG010000591         3719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_704425, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000591 DATGFG010000000
VERSION     DATGFG010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704425"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VKQ52_11040"
     CDS             <1..340
                     /locus_tag="VKQ52_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87771.1"
                     /translation="SAGNIVVTVNSSCKEFPLKREDQVIVFRIIQEALHNAIKHAEAT
                     RIGINLGWDGQRLLILVRDNGKGFDAGRTQNQGVGVINMKRRTRLLNGTISWPSDAAE
                     GTTVSISIPT"
     gene            352..990
                     /locus_tag="VKQ52_11045"
     CDS             352..990
                     /locus_tag="VKQ52_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87772.1"
                     /translation="MKITIGITDDHALFLHSLSALISSIPSFQVVLEALNGEELLTQL
                     RRGSHPDIVLLDVGMPVLDGVRTALVLAKEYPAIKTVALSMKADDLSIISMLKAGCCA
                     YLLKEIRPDELEKALMEIYTHGYYNADAINLNYRRIIQKSAEDWAQALSAREKEFLRH
                     ACSDLTYKEIATLMFVAERTIDGYRDALFQKMGVKSRVGMVLEALRHDLVSL"
     gene            1077..>3719
                     /locus_tag="VKQ52_11050"
     CDS             1077..>3719
                     /locus_tag="VKQ52_11050"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HLZ87773.1"
                     /translation="MPSLHEEIFVSYADEDKGWAQGYLIPLLQDLGWKTSTHEQFQPG
                     RSKMDELERAIGTAQYTFLVFSPAYLHDTWTSLLEQLASFFSVRERQYSLIPVMYKKC
                     ELPLRISFRVMLDFSDPAGWEREKKRLLDFLCAGAVDKRQALPCPYPGMRPFAADRAD
                     QFFGRDTETEQVIRIIRNQQFLCILGASGSGKSSLIFAGVLPRLARSALFEKDFWLVR
                     DMRPGPTPLQKIRQVVGDDCADVPAAVRNLLASQPPAKRLLLIIDQFEEVFGAVSKEE
                     SEGFIRIVEAMRRLEDCCVIITMRAAFYQDLMASSLWPVTVAERIELVPLRGKELAEA
                     IERPALQAGVYLEAGVLDLIIHDAANEPGALPLIQEVMILLWDKREGLLITQNAYRSL
                     GKSGLAVALAAKADATFLCLTTERQRIAKRIFLRLIQFGEGRPDTRRQQSSTALYSLE
                     DEKADFSYCLLNLVNNRLLTLSGEENDKAMDVDIAHESLIDGWPLLHTWIAERREAEQ
                     ARRRLQQKAEEWIRLGGQEAGLLGAVELAEANTWIRSYDARELGYGEDIEGLITASTA
                     AMEEAERLKRAAKEKEVARLRRFAMGLAIAFLVALSAGAVAYMELHIARSRQDAAQAL
                     GIKDHPSEALTLGRSGYLKNVNDETRGSLLATLQQSSQILKFLHTNHSFSWSVRISPD
                     GRTLVSGHSDGTIIFWDLKTYRPSGPPHKEHKRSVNVVDYSRDGRFLASASADGTVLL
                     WDAPHARVIRTLYHDPGNNIAALSFSPDGHTLAIAGTTKDIFLLDVLTGKRIGDPLQA
                     HTATVNVVAFSPNGKLLASGSNDETIILWDVQSRTPIGDPLDDPLGGVLALAWSPDGT
                     RLASGAQSASSGDVSENLILWDIAT"
BASE COUNT          777 a         1093 c         1122 g          727 t
ORIGIN      
        1 ttccgccggg aacatcgtcg tgacggtgaa ctcttcctgc aaggaatttc cgctgaagcg
       61 ggaggaccag gtgatcgttt tccgcatcat ccaggaggcg ctgcacaatg cgatcaagca
      121 tgccgaagcc acgcgcatcg ggatcaacct cggctgggat ggccaacgcc tccttatcct
      181 ggttcgggac aacgggaagg ggttcgatgc gggcaggacc caaaaccagg gtgtcggggt
      241 catcaatatg aaacgtcgta ccaggctcct caacggcacc atttcctggc cgtccgatgc
      301 cgcggaaggc accacggtga gcatctccat accaacttaa aatttacatg tatgaaaata
      361 accatcggca tcacggatga tcacgcgctt ttcctccatt cgcttagcgc gctcatcagc
      421 agtattccct cttttcaggt ggtgttggag gccctgaatg gcgaggaact gctgacccag
      481 ctgaggcggg gatcgcaccc cgacatcgtc ctgctggacg taggcatgcc cgtgctcgat
      541 ggcgtcagga cagcccttgt gttggcgaaa gagtatcccg ccattaagac cgtggcgctc
      601 tccatgaaag cggatgacct gtcgatcatc agcatgctga aagccggctg ctgcgcctat
      661 ctgctcaagg agatccgccc ggacgagctc gagaaggccc tgatggagat atatacccat
      721 gggtactaca atgccgatgc catcaatttg aattaccgtc gcatcatcca aaagtcggcc
      781 gaagactggg cccaggcgct ttccgcccgc gaaaaagagt tcctcaggca tgcttgcagt
      841 gacctcacct ataaagagat tgccaccctg atgttcgtcg cggaacgcac catcgatggc
      901 taccgcgacg ccctctttca aaagatgggc gtcaagagca gggtaggcat ggtgctggaa
      961 gccctccggc acgacctggt gtcgctgtga ggacccggtt ctcgctatga cgagctggtt
     1021 tcgctttgac gacccgggat cacgctgatg aattgataac ccctaattag tagcatatgc
     1081 cttccttgca tgaagaaata ttcgtttcat atgcggatga ggacaaaggc tgggcccagg
     1141 gctacctgat ccccttgctg caggacctcg gatggaagac ctccacgcat gagcaatttc
     1201 aacccggccg gtccaaaatg gacgagctcg agcgggcgat cgggactgcc cagtatacct
     1261 tcctggtatt tagcccggcc tatctccatg acacctggac ttccctgctg gagcagctgg
     1321 cctccttctt tagcgtgaga gaacggcaat acagcctgat cccggtgatg tacaaaaaat
     1381 gcgagctgcc cttgcggatc agcttccggg tgatgttgga tttcagtgat ccggcggggt
     1441 gggagcggga gaagaaaagg ttgctggact tcctgtgtgc cggcgcggta gataaacggc
     1501 aggcgctgcc ctgtccctat ccgggtatgc ggccgtttgc ggcggacagg gcggatcaat
     1561 tcttcggccg cgacacggag acagagcagg tgatccgcat catccggaac cagcaattcc
     1621 tttgcatcct gggtgcttcg ggttccggca agtcttccct gatctttgcc ggcgtgctgc
     1681 cccggctcgc ccggtctgcc ttgtttgaaa aagacttctg gctggtgcgg gatatgcggc
     1741 cgggtccgac tcccctgcaa aagatcaggc aggtggtcgg cgatgattgc gccgatgtgc
     1801 ctgccgccgt ccgtaacctc cttgcctcgc aacctcctgc gaaacgcctg ctgctgatca
     1861 tcgatcagtt cgaagaggtg ttcggcgccg tgagcaagga ggagtcggaa gggttcatcc
     1921 gcatcgtgga ggccatgcgc cggctggaag attgctgtgt catcatcacc atgcgggccg
     1981 ctttctacca ggacctgatg gccagcagcc tgtggccggt gaccgtcgcc gaacgcatcg
     2041 agctggtacc cttgcgggga aaggaactgg ctgaggccat cgaacgcccg gcgctccagg
     2101 ccggcgtata cctggaagcg ggtgtcctcg acctgatcat ccacgatgcc gcgaacgaac
     2161 cgggcgccct cccattgatc caggaagtga tgatcctgct ctgggacaaa agggaaggct
     2221 tgctcattac ccaaaacgct taccggtcgc tgggaaagag cgggctggcg gttgccctgg
     2281 ccgcgaaggc ggatgccacc ttcctgtgcc tgaccaccga gcgccagcgg atcgccaagc
     2341 gcattttcct gcggctcatc caattcgggg aaggccggcc ggatacccgg cgccagcagt
     2401 cttccacagc attgtacagc ctcgaggacg aaaaggccga tttctcctac tgcctgctca
     2461 atctcgtgaa caatcgtctc cttacgctca gcggggagga gaacgacaag gcgatggatg
     2521 tggacatcgc gcacgaatcc ctcatcgacg gatggccgct gctccatacc tggatagccg
     2581 agagaaggga ggcggaacag gcgagaaggc gattgcagca aaaggcagag gagtggatcc
     2641 ggctgggcgg gcaggaagcg gggttgctgg gcgcggtcga actggccgaa gcgaatacct
     2701 ggatccggag ctatgatgcc cgcgaactgg gctatggaga agacatcgag ggcctcatca
     2761 ccgccagcac tgcggcgatg gaggaagcgg aacggcttaa acgggcggca aaggaaaaag
     2821 aagtggccag gttgcgccgg ttcgcgatgg gtctggccat tgccttcctg gtggccctgt
     2881 cggcaggagc cgtcgcctac atggaactgc atatcgcgag atcccgccag gatgcggcgc
     2941 aggcccttgg gatcaaggac catccttccg aagcgcttac gcttggccgg tcgggatacc
     3001 tcaaaaatgt caacgacgaa accaggggca gtctcctggc caccctgcag cagagttcgc
     3061 agatattgaa attcctgcac accaaccata gttttagctg gagcgtcagg atcagcccgg
     3121 acggccggac gctggtgtcc ggccacagcg acggcaccat catcttctgg gacctgaaga
     3181 cctaccggcc ctcggggccg ccgcataaag agcataaacg ctcggtgaat gtcgtggact
     3241 acagcaggga cggcaggttc ctggcgtctg cttccgccga cggcaccgtc cttctttggg
     3301 atgcaccgca tgcccgggtg atcaggaccc tctaccatga ccccggcaac aatattgccg
     3361 cactctcttt cagcccggac gggcatacgc ttgccatcgc gggcaccacc aaggatatct
     3421 ttctcctgga tgtcctaacg ggtaagcgaa taggtgatcc gctgcaggcg cacaccgcca
     3481 cggtcaatgt ggtcgccttc agtcccaacg gaaagttgct ggcttccggc agtaacgatg
     3541 agacaatcat attgtgggat gtgcaaagcc gcacgcccat cggcgacccc ctggacgatc
     3601 cccttggcgg ggtgctggca ttggcctgga gtcccgacgg tacccggctc gcttcggggg
     3661 cccaatccgc atccagcggg gatgtttccg agaacctgat cctctgggat attgcaacg
//
LOCUS       DATGFG010000592         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_955082, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000592 DATGFG010000000
VERSION     DATGFG010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955082"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VKQ52_11055"
     CDS             <1..819
                     /locus_tag="VKQ52_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLZ87774.1"
                     /translation="IHNLSPRKSQPLVKVNCAALPTNLIESELFGHEKGSFTGATERK
                     IGKFEKADKGTIFLDEIGEMPPELQVKLLRVLQEKEIERIGSNSSTRVDVRIIAATNR
                     NLEKEVAEGRFRLDLYYRLNVFPVLLPALRERKEDIPLLAAHFIHYFNRKAGKKITGL
                     SDKVAKNMMAYSWPGNIRELQHVIERSILLAKGTLIEDVALPGIPAKQAEAPREETRL
                     KTIHEMERDHIISVLKKCNGRIWGPGAAAEVLNLPPTTLKSKMIKLGIKKEYTK"
     gene            complement(910..1743)
                     /locus_tag="VKQ52_11060"
     CDS             complement(910..1743)
                     /locus_tag="VKQ52_11060"
                     /inference="COORDINATES: protein motif:HMM:NF005395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ87775.1"
                     /translation="MKQVIVLVGAGSIGQAITRRVSAGKQVLLADLRQDNVDAAAKTL
                     SDAGFEVTTATVDVSKRASVEALVEKATSLGEVFGVVHAAGVSPSQASPEMILHVDLY
                     GTALLLEAFGKVIVRGGSGVVIASQSGHRLPPLSVEQNAALATTPVEELLGLPFLQPG
                     QVKDSLHAYQLAKRGNSLRVMAEAVRWGKRGARLNTISPGIIITPLANDELKGPRGDG
                     YRRMLEVSAAGRAGTPDEVGNLAALLMGPDGAFITGSDFLIDGGVTAAYWYGDLAPKQ
                     S"
     gene            complement(1755..2810)
                     /locus_tag="VKQ52_11065"
     CDS             complement(1755..2810)
                     /locus_tag="VKQ52_11065"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HLZ87776.1"
                     /translation="METKQVNAFGTGAADKPLQSVQIRRRMVGAHDVEMEILYCGICH
                     SDLHQIKNDFGGTTYPIVPGHEIVGRVTAVGDHVKDFKVGELAAVGCIVDSCGHCEYC
                     KDGIEQFCDEGCTYSFNSPDKVLGGVTYGGFSKTYVCNERYVLHMPDFKDLAAAAPLL
                     CAGITVYSPLKHWGTGPGKRVGILGIGGLGHLAIKIAKAMGAHVVVFTTSASKVEDAK
                     RLGADEAVLSTDASQMAKYARSLHFIIDTVSAKHDVNPYLNLLRHDGAVVLVGLPPQP
                     LEVSAFSVVLGNRSFAGSNIGGIAETQEMLDFCAKHTIVAEIEKIDMDYVNTAFERLE
                     KGDVHYRFVIDMASLKN"
BASE COUNT          775 a          921 c          752 g          654 t
ORIGIN      
        1 atccataatc tttctccccg taaaagccag ccgcttgtaa aagtgaattg cgcggcgctc
       61 ccgacaaacc tgatcgaatc cgaattgttc gggcacgaaa aaggatcatt caccggggcc
      121 acggaaagaa agataggcaa gtttgaaaag gcggacaaag gaacaatatt cctggacgag
      181 atcggcgaaa tgccccccga gctacaggta aagcttctgc gggtactgca ggaaaaggaa
      241 attgaacgca tcggatcgaa ctcctccacc cgggtagacg tccggattat tgccgcaact
      301 aaccgcaacc tcgagaaaga agtggctgaa ggacgcttcc gcctcgattt gtattaccgg
      361 ctcaatgtct tccccgtact actgccggcg ctacgcgaac gtaaagagga cattccctta
      421 ttagcggccc attttataca ttattttaac cgaaaagcgg gcaaaaaaat caccggcctc
      481 tcggataaag tggcaaagaa tatgatggct tacagttggc ccgggaatat ccgggaactg
      541 cagcatgtaa ttgaaagaag catcctgctg gcaaaaggga cattgatcga agatgtggca
      601 ttaccgggga tcccggcaaa acaagcagaa gccccaaggg aagaaacccg gctcaaaacc
      661 attcatgaaa tggaacggga tcatattatc agcgttctaa agaaatgtaa tggtaggatt
      721 tggggccccg gggcagcggc ggaagtgttg aacctgcccc cgactacgct gaaatcgaag
      781 atgataaagc tgggcattaa gaaggaatat acgaaatgac aggtccagcc ggtgctttct
      841 ctatgatctc cacgatgacc cgccgggcaa tattgccgct ggcgccaatg atgatgacat
      901 ttttcataat cagctttgtt tcggtgccaa atctccatac caataagccg ccgtcacccc
      961 cccgtctatc aggaagtcgc tgccggtgat aaaggcgccg tccggtccca tcagcaaagc
     1021 ggccaggttg cccacctcat ccggcgttcc ggccctgccc gcagcggaca cctccagcat
     1081 acgccgataa ccatctcccc gcgggccctt cagctcgtca ttcgccagcg gggtgataat
     1141 aatccccgga ctgatcgtgt tgagcctggc gccgcgtttg ccccagcgca cggcctccgc
     1201 catcacccgc aacgaatttc cgcgctttgc cagctggtaa gcgtgaagcg agtccttcac
     1261 ctggccgggc tgcaggaacg gcagacccag caattcctcg accggcgtgg tcgccagggc
     1321 ggcgttttgc tctacagaca gcggaggcag ccggtgaccc gactgcgacg cgatcacaac
     1381 gccggaccca ccgcggacga tcaccttccc gaacgcctcc aggagaaggg cagtaccata
     1441 tagatcgaca tggaggatca tttcaggcga tgcctgagac ggggacaccc cggcggcatg
     1501 aacgacgcca aacacctcgc caagcgatgt ggccttctct acaagggcct ccactgaagc
     1561 ccgcttcgac acatctacgg tcgccgtggt tacttcaaat cccgcgtcgc tcaaggtctt
     1621 cgccgcggca tcgacgttat cctgtcgtag atccgccagt aaaacctgct tacccgcact
     1681 aacccgccgg gtaatcgcct gcccgatcga tccggcgccg actagcacaa tgacttgttt
     1741 catgatactt ggtattaatt tttcaaagac gccatatcga tcacaaaccg gtaatgaaca
     1801 tccccctttt ccagtcgttc aaaagccgta ttcacataat ccatatcgat cttttctatt
     1861 tcagccacga tagtatgttt cgcacagaag tccagcatct cctgggtttc tgcaatcccg
     1921 ccgatattgg aaccggcaaa gctcctgtta cccaggacga cgctaaaggc acttacttcc
     1981 aacggctgcg gaggcaggcc caccagcact acggccccgt catgccgcaa caaattcaga
     2041 tacggattta catcgtgctt tgccgaaacc gtatcgatga taaagtgcag gctacgcgca
     2101 tacttcgcca tctggctggc gtcggtcgac aagaccgcct cgtctgcgcc cagccgcttt
     2161 gcatcctcga ccttcgacgc agaggtcgtg aacactacca cgtgcgcccc catggccttc
     2221 gcaatcttga tggcaaggtg tcccagaccg ccgataccca atatgccgac ccttttgccc
     2281 ggacctgtgc cccaatgttt caaaggggaa tacaccgtaa tcccggcgca caagagcgga
     2341 gccgctgccg ccaggtcctt aaaatccggc atatgcagca cgtaccgttc gttacataca
     2401 taagtcttgg agaaaccgcc ataggtgacg ccgcccaaaa ccttatccgg gctattgaac
     2461 gaataggtac atccttcatc gcagaactgc tcgataccgt ccttgcaata ttcacaatga
     2521 ccgcagctgt ccacaataca acccaccgcg gccagctcac ctaccttgaa gtcctttaca
     2581 tggtcaccca cagccgtcac ccgaccaacg atctcgtggc ccggcacgat cggataggtc
     2641 gtaccgccaa aatcgttctt gatctgatgc aggtccgagt gacagatccc gcagtacagg
     2701 atttccattt cgacgtcgtg ggcacccacc atccggcgac ggatctggac ggattgtaga
     2761 ggcttatcgg ccgcccccgt tccgaaggcg ttaacttgtt ttgtttccat tatagtgatg
     2821 tttaattatc ccggcgcaaa ggtagcacca tctccgcccc ggctgctaac tcatatcaaa
     2881 cggcttttta cgaaaatcaa acaattcctg aggtcgcctg cgtatccgcc acatctatgc
     2941 cggaattccg gcttttacca actacttcgg tacgtttgaa atttgtaaat ttgcagtatg
     3001 gagaaagtgc aacccgtaga ggaattacac tcttcaaaaa aggaagggat acctgactgt
     3061 atcggtaacg atattggaca gtttaatgta ttccgcctgg ac
//
LOCUS       DATGFG010000593         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_441938, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000593 DATGFG010000000
VERSION     DATGFG010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441938"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..>2016
                     /locus_tag="VKQ52_11070"
     CDS             252..>2016
                     /locus_tag="VKQ52_11070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HLZ87777.1"
                     /translation="MKSSIIKITTPSLLACLTGLSLSAQQPAHRLPKEAAPLAYVDPY
                     IGTGGHGHVFLGASVPFGAVQAGPMNINKGWDWCSGYHYSDSVVKGFAQNHLNGTGIP
                     DLGDILVMPFTGAVRTDVGTQQDPASGYASHYDHRGEIARPAYYSLWLKDHKVNVELT
                     ATERVAFHRYRFPKDRPAHIIIDLLQGNFDAGWQHPKVKAHLLKLNDSTLIGWRQSTQ
                     WAKDRLIYFAIRTSFPLKDFSLINGNTPIRELTLEADTIKGLISFAGNPGTVMLKIGV
                     SNVSGEKALANIEAEIPRWNFEEIVRQGNEKWRRALDKIRIEAADSIRRRVFYTALYH
                     TMIAPALYNDHDSSYRGADGKVIDHAPYNNYTIFSLWDTYRTLNPLMTLIHPERVDDI
                     INTMLAIYVQQGKLPIWHLQGRETDCMVGYSAVPVIADAYLMGFRGFDANLALEAMKA
                     SSTRDDYGMNYLKQEGYIPADKERESVSKALEYAIDDWCIARMATRMGRTEDAAYYAK
                     RGEYFTRYFDTTTKFMRPVLADGHYREPFNPFQSIHEWGDYTEGNSWQYTWLVPQDVE
                     RLISMMGGDKAFVTKLDSLFVV"
BASE COUNT          464 a          616 c          517 g          419 t
ORIGIN      
        1 taacgcgatc gacgggaagg tattccctgt cgacatcgca taggtagact gcgtacccat
       61 caacgggttg acaaagtcga caggatcgcg aacgacatcg acccactggg cctgcgtagc
      121 gccggcaatg gcaagcaggg cgctaaatag aacagcgtgt tttagcatgg tattgttaaa
      181 aattttacgt cgcaagctat cgattcctgt tcagattttt acagctaatc ctgcttcggt
      241 atctttagcg catgaaatcg agcatcataa aaataacaac cccttctctc ctcgcttgcc
      301 tcaccggtct ctccctctcc gcgcaacaac ccgctcatcg tttgccaaag gaggccgcgc
      361 cgcttgcgta tgtggaccct tatatcggca ccggcggtca tggccatgtc ttcctcggcg
      421 ccagcgtccc ctttggcgcg gtgcaggcag gaccgatgaa tatcaataaa ggctgggact
      481 ggtgctcggg atatcactat agcgatagcg tcgtcaaagg ctttgcccag aaccatctca
      541 acggtaccgg gatccccgat ctcggcgata tcctcgtcat gccctttacc ggcgccgttc
      601 gcaccgacgt aggcacccag caggatccgg catccggcta tgcctcccat tatgaccatc
      661 gcggcgagat cgcccgtccc gcctactatt ctctctggct gaaagaccac aaggtaaacg
      721 tcgaactcac cgccaccgaa cgcgtggcct ttcatcgcta ccgctttcca aaggatcgtc
      781 ccgcccatat catcatcgat cttctccagg gcaatttcga cgctggctgg cagcatccaa
      841 aggtgaaggc tcacctgctg aagctgaatg actctactct catcggctgg cgccaaagca
      901 cccagtgggc gaaagacagg ctcatctatt tcgccatccg caccagcttc ccgctaaagg
      961 acttctcgct catcaacggc aatacaccca tcagggagct gaccctcgag gccgatacga
     1021 taaaggggct tatttccttt gcgggcaacc cgggcaccgt gatgttgaag atcggcgtct
     1081 ccaacgtcag cggcgagaaa gccctggcca atatcgaagc ggagatcccc cgctggaact
     1141 tcgaagagat cgtccggcag ggtaatgaga aatggcgccg cgccctggac aagataagga
     1201 tcgaagccgc cgactccatc cggcgaaggg tcttttatac ggcgttgtat catacgatga
     1261 tcgctcccgc attgtacaac gatcacgaca gcagctaccg tggcgccgat gggaaggtga
     1321 tcgaccatgc gccgtataat aattatacga tcttctcgct ctgggatacc taccgcaccc
     1381 tgaacccgct gatgacgctg atccatcccg aacgggtgga tgacatcatc aacaccatgc
     1441 tcgccatcta tgtccagcag ggtaagctgc ccatctggca tctgcagggt cgcgagaccg
     1501 attgcatggt cggttacagc gccgtccccg tcattgccga cgcctacctg atgggcttcc
     1561 ggggcttcga cgccaacctt gcgctggagg cgatgaaggc ctcttccacc cgcgatgact
     1621 atggaatgaa ctacctgaaa caggaaggct atatccccgc agataaagaa cgggagtcgg
     1681 tgtccaaggc gctggaatat gccatcgacg actggtgcat cgcccgcatg gcgacaagga
     1741 tggggcgcac ggaagatgcg gcctactatg ctaaacgcgg tgaatatttt acccgctatt
     1801 tcgatacgac gacaaaattc atgcggcccg tcctcgccga cggccactat cgcgaaccct
     1861 tcaacccctt ccagtccatc catgaatggg gcgactacac cgaaggcaat tcctggcaat
     1921 atacctggct cgtcccccag gacgtcgaaa ggctgatcag tatgatgggc ggtgacaaag
     1981 ccttcgtcac gaaactggac agcctctttg tcgtca
//
LOCUS       DATGFG010000594         4413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_229984, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000594 DATGFG010000000
VERSION     DATGFG010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229984"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VKQ52_11075"
     CDS             <1..875
                     /locus_tag="VKQ52_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87778.1"
                     /translation="LGADAMYSNTTGLGNTATGASALTFNTIGSFNTANGYSALGFSK
                     TGIQNTAIGAYVLNYNSSGSNNTASGYQSLPQNDAGSSNTANGAASMYFNTEGNNNVA
                     VGSSSLHQNITGNNNTALGQGAGYLSSGDGNVFIGNQAGFSERTGSNKFYVGNDQLHT
                     LLYGDFSTGQVLLGNLNPTGYVFAGTRTLNVTGGVLADSIRLAPSDQWADFVFDSSYA
                     LPSLGEVGAFVKEKRHLPGIPSAGEVAVSGVSLGEMNQKLLQKVEELTLYLIQQQKEI
                     AELKSMVKELAVKK"
     gene            913..2289
                     /locus_tag="VKQ52_11080"
     CDS             913..2289
                     /locus_tag="VKQ52_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ87779.1"
                     /translation="MSISMKLILPAVLFLPMFCVQKAGAQLIVQNGASLITNGAGHVA
                     LQNTGLVCNGTVSQGPGGTFSFSGSGNDSITGSMPPLFDQLEIAKAGGARLLLKQGLT
                     VNSSVLFSGGIIDLDGNILSLSSAASLTGEGETSYITGAGGGSVQIATTAAGAPSALN
                     LGNLGAVITSGSDLGALQVSRWHVPAVNPANGAMTGIQRSFAITPANDVALNATLRFY
                     YLNEELNGKDPNGLGLWSSGDGVKWSFVGADSRDATQKFVEKAGIASFSVWTLSDATS
                     PLPVRLINFSAVCGSNDVVVKWTTAGETGFADFVVEKSSNASDWIAIATIPGSNDPLG
                     ASYGYTDAQPAATAYYRLKMVDKDGQFTYSPVFSGGCSDLTMPFALYPNPAVSSITAR
                     ISVRQGSAAAISVFNAAGQAVETIGVSLVPGANSIPLPINSLPAGIYFVQAVMAGGEI
                     QRGAFVKK"
     gene            complement(2302..3090)
                     /locus_tag="VKQ52_11085"
     CDS             complement(2302..3090)
                     /locus_tag="VKQ52_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ87780.1"
                     /translation="MPEEKINILIVEDESLVALDLATGLEKAGYHVVGTADHAEEALQ
                     IFGANEVDIVLMDIHLAGKKDGIDTVVDMMKIRQTPVIYLTAYTDAATIQRVKQTHPA
                     AFLLKPYSIANVRIAIELAFHQLAVLRQQKGQGRVIPLEETGGGKKEGAAKESILQLD
                     DSIFFKVNHQFIKLRLTDILYVEADGNHLNIFTTERKFVLRLSLNQLFEKLRFKKLVR
                     IHRSYAVNIDTIQSFNDMEVVTGKRQLPIGRNYKEEFLRNFDFR"
     gene            complement(3149..>4413)
                     /locus_tag="VKQ52_11090"
     CDS             complement(3149..>4413)
                     /locus_tag="VKQ52_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HLZ87781.1"
                     /translation="SALTCYSLALQLAEHSEKGRNLTGRLYNNISQVYAEYKKDYPAA
                     LQALEKAAAFNQQRKNNKSLTFTYENMADVYSRMGDNRRSLEYAGKTLALARQLNFPE
                     RIENAYLQLHASYKAIGRYDSALYYYTLYHHLSDSLVNLDKNSQMAEMQTRYETAKKE
                     AEIGQLNRDNRDKNRQILLLVAGLVIVCLVAAAMLWLYRRVNRQKGVISRQSQQLEMV
                     MKELHHRVKNNLQIVSSLLSLQSYKLSDEEAIAAIRQSQQRVQAMSLIHQRLYKTDES
                     AFVNIKEYLTDLAESLLSSYGFDKEQFDLRITAEQKLLDVDKVLLLGLIVNEILTNAF
                     KYAFKNIDRPDRPSLRITCTGKDGEFILSIKDNGPAWDATKWKETGGSFGKQLVTALC
                     RQLRATQELTVQEGTQFIFRIPLEAQIA"
BASE COUNT          880 a         1148 c         1332 g         1053 t
ORIGIN      
        1 ccctgggagc tgatgctatg tatagcaata ctacgggcct tggcaacacc gccaccggcg
       61 ccagcgccct gacgtttaat accatcggct cttttaacac ggccaatgga tattcggccc
      121 ttggcttcag taagaccggc attcagaata ccgccatcgg agcatatgtg ctaaactata
      181 actcttcggg ctccaacaac acggcctcgg gatatcagtc gcttccgcaa aatgatgcgg
      241 gctcaagcaa tacggccaat ggagccgcat cgatgtattt caataccgag gggaacaata
      301 acgttgcggt gggttcgtct tcccttcacc aaaacattac cggcaacaat aatactgcgc
      361 tgggccaggg ggcagggtac cttagctcgg gcgacgggaa tgtcttcatc ggcaaccagg
      421 cgggcttttc ggagcgcacc ggcagcaaca agttctatgt cggcaatgac cagttgcata
      481 ccctgctata cggggatttc agcacggggc aggttttgtt ggggaacctc aacccaacgg
      541 ggtatgtttt cgccggcacg cgaacgctga atgtcaccgg gggcgtgctt gcggacagta
      601 tccggctggc tcccagcgat cagtgggccg actttgtatt cgacagcagc tatgcgctgc
      661 cctcgcttgg agaagtaggt gcgtttgtaa aagaaaaacg gcatctcccg ggcatcccca
      721 gcgcgggcga ggtggctgtc agcggcgtca gccttgggga gatgaaccaa aagctgctgc
      781 aaaaagtgga ggagctgacg ctttacctta tccagcagca aaaggagatt gccgagttaa
      841 aatccatggt caaagagctg gccgttaaaa aatagaccgg agaacgggtt ttatccggga
      901 aaaaatacca aaatgagcat ttcaatgaag ctgatcctac cggcagtcct tttcctgccg
      961 atgttttgtg ttcagaaagc cggtgcgcaa ctcatcgtgc agaatggcgc atcgctgatc
     1021 accaatggcg ccgggcatgt cgcgttgcag aacaccgggc tggtctgtaa tggaaccgtg
     1081 agccagggcc cgggcggcac gtttagcttt agcggcagcg gcaatgactc catcaccggg
     1141 agcatgccac cgctgttcga ccagctggag atcgccaagg ccggcggcgc caggctattg
     1201 ctgaagcagg ggctgacggt gaacagcagt gtgctcttca gtggcggcat catcgacctg
     1261 gacgggaata tcctgtcgct gtcatccgcc gcgagtctta ccggggaagg cgagaccagc
     1321 tatattaccg gcgccggggg cggcagcgtg cagatagcta ccaccgctgc gggggcgccc
     1381 tccgcgctga acttaggcaa cctgggcgcc gtcatcacca gtggcagtga ccttggcgcc
     1441 ctccaggtgt cgcggtggca tgtccccgcg gtgaacccgg cgaacggcgc gatgaccggc
     1501 atccagcgca gctttgcgat cacgcccgcc aatgacgtgg ccctgaacgc gaccctgcgc
     1561 ttctactatc ttaatgagga gctgaatggc aaggacccca acgggctggg tctctggagc
     1621 agcggcgatg gggtgaaatg gtcctttgtc ggggcggaca gccgggatgc cacgcagaaa
     1681 ttcgtggaaa aagcgggcat tgccagcttc agcgtctgga cgcttagcga tgctaccagc
     1741 ccgctgccgg tgcggctgat caattttagc gcggtgtgcg ggagcaacga cgtggtggtg
     1801 aaatggacga ccgccggcga aacgggtttt gccgattttg tagtggaaaa aagcagtaat
     1861 gccagtgact ggatagcgat tgccacgata ccgggcagca acgatccttt gggggcttct
     1921 tacggttata ccgatgccca gccggcggcc accgcctatt accggctgaa gatggtggat
     1981 aaggacgggc aatttaccta ttctcccgtc ttcagtggcg gctgtagcga cctgaccatg
     2041 ccgtttgccc tttatcccaa ccccgctgtg agcagcatca cggcccggat atcggtgcgg
     2101 cagggttcgg cggcggcgat cagtgtattc aatgcggcgg ggcaggcggt ggagacgatc
     2161 ggggtgtcgc ttgtgccggg cgccaattcg atccccttac cgatcaattc gcttcccgcg
     2221 ggcatctatt ttgtgcaggc ggtgatggcg ggtggggaga tccagcgggg ggcgtttgtg
     2281 aagaaataga actttttcgg attaccggaa atcgaaattc cggaggaatt cttccttgta
     2341 gttccggccg atggggagtt gccgtttgcc ggtgaccact tccatatcgt tgaacgattg
     2401 gatggtgtcg atattgacgg cataggagcg atggatgcga acgagttttt tgaagcggag
     2461 cttttcgaac agttggttga gtgacagcct gagtacgaac ttccgttccg tggtgaagat
     2521 attgaggtga ttgccgtccg cttcgacgta gaggatatcg gtgaggcgga gctttataaa
     2581 ctggtggttg accttgaaga aaatgctatc atccagttgc agaatggatt ctttggccgc
     2641 cccttccttt ttcccgccgc cggtctcttc cagggggatg accctgccct gccctttttg
     2701 ctgccggaga acggccaact gatggaaggc cagctcgatg gcgatcctta cattggcgat
     2761 gctgtatggt ttcagcagaa atgccgcggg atgggtttgt ttgacccgct ggatggtagc
     2821 cgcgtctgtg tatgccgtga ggtagatgac gggtgtctgc ctgatcttca tcatatccac
     2881 caccgtatcg atgccatcct ttttgccggc cagatggata tccatcaaga caatatccac
     2941 ttcgttggcg ccgaagatct gtagcgcttc ttcggcatga tccgccgtgc cgaccacgtg
     3001 gtagcccgct ttttcgaggc ctgtcgccag gtccagcgcc accagggatt cgtcttcgac
     3061 gatgaggata tttatttttt cttccggcat tgctagttca tttgttcggc gtttttccga
     3121 tgttctgcgt tttttgcggc caggcgtatc aggcgatctg tgcttccagg gggatgcgga
     3181 aaatgaattg ggtcccctcc tgaacggtca attcctgggt ggcgcgcaat tgcctgcaca
     3241 gggccgtgac gagttgtttg ccaaaggagc cgcccgtctc cttccatttg gtggcatccc
     3301 atgccggtcc gttatccttt atcgaaagga tgaactcccc gtccttgcct gtacaggtga
     3361 tgcgcagtga agggcgatcg gggcgatcga tattcttaaa ggcatattta aaagcgttgg
     3421 taaggatctc attgacgatg aggcccagca agaggacctt gtcgacgtcc agcagcttct
     3481 gttccgcggt gatccggagg tcgaactgtt ctttatcaaa cccgtaggaa gacaacaggg
     3541 attcggcaag atcggtgagg tactctttta tattgacaaa ggcggactcg tccgttttgt
     3601 acaggcgttg gtggatgagg ctcatggcct gtaccctttg ctggctctgg cggatggcgg
     3661 caatcgcctc ttcgtcgctc agcttgtagg attgcaggct gagcaggctg ctgacgatct
     3721 gcaggttgtt ctttaccctg tgatgcaact ccttcatcac catttccagc tgttgggatt
     3781 gccggctgat cactcccttc tgtcgattta cccgccggta taaccaaagc atcgcggcag
     3841 cgacgaggca gacgatcacg agaccggcga ccagcagcag gatctgcctg ttcttatccc
     3901 ggttgtccct gttcagctga ccgatctccg cctctttctt cgccgtctcg tagcgggttt
     3961 gcatttcagc catctggctg ttcttgtcga ggttaaccag gctgtcgctg agatgatggt
     4021 agagggtgta ataatagagt gcggagtcat atcttccgat ggctttgtag ctggcatgaa
     4081 gctggagata ggcgttctcg atgcgttcgg gaaaattcag ttggcgcgcc agggcgagtg
     4141 tcttccccgc gtattcgagg gagcgtctgt tgtcacccat cctgctatag acatcggcca
     4201 tattctcgta ggtgaaggtg aggcttttgt tgttctttcg ctgctggttg aatgccgctg
     4261 ctttttccag ggcttgcagg gccgccgggt aatccttttt gtattcggca tagacctggc
     4321 tgatattgtt gtacaggcgg ccggtgaggt ttcgtccttt ttccgaatgt tccgccaatt
     4381 gcaaggcgag ggagtagcag gtgagggccg aat
//
LOCUS       DATGFG010000595         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1024639, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000595 DATGFG010000000
VERSION     DATGFG010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024639"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1274
                     /locus_tag="VKQ52_11095"
     CDS             240..1274
                     /locus_tag="VKQ52_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLZ87782.1"
                     /translation="MKFEAITIKDIAKALHLSVSTVSKALRGSHEISEETKKHVLAYA
                     SEHNYKPNPIAQSLKRGRSKSIGVIVCNIDNNFFSAVINGIESVARQKDYNVIITQSQ
                     ESYEREVANSEHLSSRSVDGLIISLSAETKRVDHLIKLHDKGLPMVFFDRITDEIATH
                     KVIADNFKGAYEATRHLLQQGFRRIAHITSSGSLSITLERLEGYKKALEDAGLKSDPK
                     YIKFCPHGGMIKEETQQALTELLQLKNRPDAIFTASDRLSTTTLSLLRKMQIAVPQQI
                     ALVGFTNSISADIFHPSLTSVVQPAIEMGQLATELLIQLIEAKRPVTEFHKRVLDTEL
                     QIRDSSLRVR"
     gene            complement(1327..1980)
                     /locus_tag="VKQ52_11100"
     CDS             complement(1327..1980)
                     /locus_tag="VKQ52_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ87783.1"
                     /translation="MKVALIGATGFVGTALVNELTSRGHEVIAIARHPEKLAVKSPLI
                     HRVTADILDAQQVAKAVKGADAVVSAYNPGWTNPRIYEEFLEGSRAIQAGVKQSGVKR
                     LIVIGGAGSLEIKPGLQLIDTPQFPAEYKPGASGARDYLNEIKKEKDLDWTFFSPAIL
                     MNHDTAGVRRGHYRLGLDNPVFDEKGQSILSVEDLALVIVDELEHPKHIKARFTAAY"
BASE COUNT          418 a          517 c          604 g          466 t
ORIGIN      
        1 tgcagagacg ccgatgatgt cggcgccggc cgacgcgagc cccagcgcca tgccccggcc
       61 gatgcccttg tttgcaccgg tgaccagggc gaccttaccc ttcagattaa acagatcact
      121 cataaagata ttatcagcag ttcacttata tttgacaagt gtacacccta attcctataa
      181 gaaaggattg atcttcaact ttttttacga aaacgtttgc gcggaggttc tactattaca
      241 tgaagtttga ggctattacc ataaaagata ttgccaaggc attgcacctg tctgtatcta
      301 ccgtttcgaa ggcgctgcgg ggcagccacg agatcagcga ggagacaaaa aagcatgttc
      361 tggcctatgc cagcgagcat aattataagc ccaacccgat cgcgcagagc ctgaagcgcg
      421 ggcggagcaa gtcgatcggg gtaatcgtct gtaatatcga taacaatttc ttttccgcgg
      481 tgatcaatgg catcgagtcg gtagcgcgcc aaaaggatta caatgtcatc atcacccaga
      541 gccaggagtc atatgagcgg gaggtcgcca acagcgagca tctttcctcg cgttcggtgg
      601 atgggctgat catctcgctc tcggcggaga cgaagcgggt ggaccatctt atcaagttac
      661 acgacaaggg gttgccgatg gtcttttttg accggatcac cgatgagata gccacccaca
      721 aggtgattgc cgataacttc aagggcgcct acgaggcaac gcggcatctc ctgcaacagg
      781 ggttccggcg gatcgcgcat atcaccagca gcggcagtct gtccatcacc ctggagcggc
      841 tggaagggta taagaaggcg ctggaggatg cggggctgaa gtccgatccg aaatacatca
      901 aattctgtcc ccatggcggg atgatcaagg aggaaaccca gcaggcgttg accgagctgc
      961 tgcagctgaa gaaccggccc gatgccatct ttaccgccag cgaccggctt agcaccacga
     1021 cgctgagcct gcttcgcaag atgcagatag ccgttccgca gcagatcgcc ctggttggct
     1081 ttaccaattc catttccgcg gatatcttcc atccttccct gacctcggtg gtccagccgg
     1141 cgatcgagat gggtcaattg gccacggagc tgttgatcca gctcatcgag gccaagcggc
     1201 ccgttaccga attccacaag cgggtgctgg atacggagtt gcagatcagg gattcgagcc
     1261 tgcgtgtgcg ttagtttcgg tgcccgttag ccagcgtgtg cgttaggggc gcatagatgc
     1321 ctgagatcaa taagccgcgg tgaaccgggc cttgatatgt ttgggatgtt ccagttcatc
     1381 gacgatgacg agggccaggt cttccacgga gaggatgctt tggccttttt cgtcgaagac
     1441 ggggttgtcg aggcccaggc gatagtggcc ccggcggacg cccgcggtgt catggttcat
     1501 gaggatggcg gggctgaaga aggtccagtc caggtctttt tcctttttta tctcgttgag
     1561 atagtcacgg gcgccggacg caccgggttt gtattcggcg gggaattgcg gggtatcgat
     1621 cagttgcaga cccggtttta tttcgaggct gccggcgccg ccgatgacga tgaggcgttt
     1681 tacgccggat tgtttgacgc cggcctggat ggcgcgcgat ccttcgagaa attcttcata
     1741 tatccggggg tttgtccagc cggggttata ggcgctgacg acggcatcgg ccccttttac
     1801 tgctttggct acctgttgag cgtcgaggat gtcggcggtt acgcggtgga tgagcgggct
     1861 ttttactgcg agcttttccg ggtggcgggc gatggcgatg acctcgtggc cgcgggaggt
     1921 caactcgttt acgagggctg ttccaacgaa gccggtggct ccgatcaggg ctactttcat
     1981 tttgtcggtg atttagttac ttttg
//
LOCUS       DATGFG010000596         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_261220, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000596 DATGFG010000000
VERSION     DATGFG010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261220"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VKQ52_11105"
     CDS             complement(<1..1316)
                     /locus_tag="VKQ52_11105"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ87784.1"
                     /translation="MNLKQGYRKYAGGIVLPHLAVWSLLVAAGLELLAAGVWGLLLAA
                     GLGLLPVDAAAQGPTKNVALVGRVSDEKTGDPLPGAVIHIKGTTHEVAADNNGEFRFI
                     TGQRVPVTYSVSYVGYKTREIEVTNYEHAELKLQGGNAQLNDVVVVGYGVQRRSDVTG
                     AVAGVGKSALSQPAVSLDNLLQGSVSGVAVTQNNSQPGSTAAIRVRGGNSISFSNDPL
                     YVIDGFIVYNNNNYTNSGATSGAGVNALSTINPSDIESIEVLKDASATAIYGSHGANG
                     VVIITTRRGRKGTDEVNYSGYYGSQSAVKKIGLLNATQWGSLVNDVNVSDGVAKTYTD
                     AQLAALGKGSDWQSAGIRKAPVQNHELSVSGGDDRSKYLLSGNYFDQDGILYNTGFKR
                     YSGRFNYERNVTDLLKLNLSAFGSHSAENSLVGSAYTSINLSNAWST"
     gene            1659..2414
                     /locus_tag="VKQ52_11110"
     CDS             1659..2414
                     /locus_tag="VKQ52_11110"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLZ87785.1"
                     /translation="MTTTILEQLALIEQNEGVRILYACESGSRAWGFPSPDSDYDVRF
                     IYAQPRDNYISIRDPQDVVEIPVNPVLDINGWDIRKTLQLFLKSNSPLYEWLQSPIGY
                     KEEGGFAAELRRLMTEYYSHRSGCHHYLSMAYNTLNNDLKGNEVKLKKYFYALRPALA
                     CGWILEKRTVPPMEFAVLRTLITDSAWNATVDELLRQKARADEKALIAPIPLLQHWLS
                     NTLEQFKNRADTIPAVKHDTTRLDELFRKTIVP"
     gene            2447..>3110
                     /locus_tag="VKQ52_11115"
     CDS             2447..>3110
                     /locus_tag="VKQ52_11115"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLZ87786.1"
                     /translation="MTFHQLIREPQHLLLKCISGSRAYNLALPASDTDIKGIFILPRR
                     ELYGLNYTPQVSNETNDEVYYEIGRFIDLLSKNNPNILELLSTPETSVLFRHPLMHLI
                     RPQDFLSKLCLDTFAGYAKTQIKKARGLNKKINRSFEKERRTILGFCHVVEGNATVPL
                     AAWLDAKGLDQADCGLVNLAHFRDAYLLYHEGHPPPQSQHPGLSPHSPPPQPPSQTPP
                     TAT"
BASE COUNT          695 a         1038 c          753 g          624 t
ORIGIN      
        1 gtcgaccagg cattggaaag attgatggaa gtataggccg agcccacgag ggaattctcg
       61 gcggaatgac tgccaaaagc gctcaggttc aacttgagaa gatcggttac atttcgttcg
      121 tagttgaagc ggcccgagta acgcttgaac cccgtgttgt acaagatgcc gtcctggtcg
      181 aaataattgc cgctgaggag atacttgctg cgatcgtcgc cacctgacac ggacagttca
      241 tggttttgca ccggtgcctt gcggataccc gcgctctgcc agtccgagcc cttgcccagc
      301 gcggccaatt gggcgtcggt ataggtcttg gccacgccgt cgctgacatt gacatcgttc
      361 accagcgagc cccattgcgt ggcgttgagc aggccgatct ttttgactgc ggactgggag
      421 ccatagtaac cggaatagtt tacttcgtcc gtccccttcc ggccccggcg ggtggtgatg
      481 atcacgaccc cgttggcgcc atgggagcca tagatggccg tggccgaggc gtctttcagg
      541 acctcgatcg actcgatatc gcttggattg atggtcgaca gcgcattcac tcccgcgccg
      601 ctcgtcgcgc ccgaattggt gtagttatta ttgttgtaga cgatgaagcc atcgatcaca
      661 tagagcggat cgttgctaaa ggagatggag ttcccgcccc gcacccggat agcggccgtg
      721 ctgccgggct gcgaattatt ctgggtcacc gccacacccg acaccgaccc ctggaggaga
      781 ttatccaacg aaacagcggg ctggctgagg gcgctcttgc caactccggc cacggcgcct
      841 gtaacgtcgc tgcgacgctg gacaccataa ccgacaacca ccacgtcgtt gagttgcgca
      901 ttgccaccct gcagcttcag ctccgcatgt tcgtagttcg tgacctcgat ctcccgggtc
      961 ttatagccta cgtagctaac cgaataggtt accggtacgc gctgacccgt gataaaccgg
     1021 aattcgccgt tattatcggc agccacctca tgtgtagttc cttttatatg gataacggcc
     1081 ccgggtagcg ggtcgcccgt cttctcatcg cttacgcgcc ccaccagtgc aacattcttt
     1141 gtgggcccct gcgccgccgc atccaccggc aaaagcccca gccccgcagc caaaagcagc
     1201 ccccagaccc ccgcagccaa aagctccaac cccgccgcta ccagcaaact ccacaccgcc
     1261 aaatgcggca atacaatgcc cccggcatat tttctatacc cttgcttcag attcatgatt
     1321 tgatatttta tcgatttatg gattcgggaa agccgctgaa aaaatctcag cagcaacaac
     1381 acccgcaccg gctgcccgca tcgaccccgg gcctcgcatc ctgaccggac gccgtccggt
     1441 tctgattcgc cgcacaaaaa attgcatgac tccgccaggc agcggaatac cctttcgcag
     1501 ttgccggttg catagacttg ataagttaat tctcagcaaa tattggaaac atttattagt
     1561 ccaccaaatt agtagacaaa taaatttttc gcgccatccc ccatcctccg cgcccccgcg
     1621 aaaaatatat tcagtttttt ccccgatatt taccatccat gacaaccacc atccttgaac
     1681 agctggccct catcgaacaa aacgaagggg tccgcatcct ctacgcctgc gaatcgggta
     1741 gccgtgcctg gggcttcccc tccccggaca gcgactacga cgtccgcttt atctatgccc
     1801 agccccgcga caactatatc agtatccgcg acccgcagga tgttgtcgag attcccgtca
     1861 accccgtgct cgacatcaac ggctgggaca tccgcaaaac gcttcaactc ttcctcaaat
     1921 cgaactctcc gctgtacgaa tggctgcagt cgccgatcgg gtataaggaa gagggcggct
     1981 tcgcggcgga gctccgccgg ctcatgaccg agtattactc ccaccggtcc ggttgccatc
     2041 attatctgtc catggcctat aataccctga acaacgatct aaaggggaac gaggtaaagc
     2101 tcaagaaata cttctacgcc ctgcggccgg cgctggcctg cggctggatc ctcgaaaagc
     2161 gtaccgtccc cccgatggag ttcgctgtcc tgcgtaccct gatcaccgac tccgcctgga
     2221 atgcaacggt ggacgagctc ctgcggcaaa aggcccgggc cgacgagaaa gccctcatcg
     2281 cgcccatacc cctattgcag cactggctca gcaacaccct ggaacaattt aagaaccggg
     2341 cggataccat ccccgccgta aagcacgaca caacccggct cgacgaactc ttccggaaaa
     2401 caatcgtgcc gtaaatggcc gccgccgccg gcttaatcgt ctatccatga ccttccacca
     2461 gctcatccgg gaaccgcaac atctcctgct gaaatgcatc agcggcagcc gcgcctataa
     2521 ccttgccctg cccgcctccg acacggacat caaggggatc ttcatcctcc cccggcggga
     2581 actctacggc ctcaactata cgccacaggt atccaatgag acgaacgacg aggtctatta
     2641 cgagatcggt cgatttatcg atctcctcag caagaacaac cccaacatcc tcgaactcct
     2701 aagcacaccc gaaacaagcg tcctcttccg gcatccgctc atgcacctca tcaggccgca
     2761 ggacttcctg tccaaactct gtctcgacac ctttgccggc tatgcaaaga cgcagataaa
     2821 aaaggcaagg ggattgaata agaagatcaa ccgcagcttc gaaaaggagc gccgcaccat
     2881 cctcggcttc tgccacgtcg tggaaggcaa cgcaaccgtc cccctcgccg cctggctgga
     2941 cgcgaaaggc ctcgaccagg ccgactgcgg actcgtcaac ctcgcccact tccgcgacgc
     3001 gtacctgctt tatcatgagg gccatccccc gccacaaagc cagcaccccg gcctttcccc
     3061 gcattctccc ccgcctcaac ccccatcgca gaccccgcct acggccactt
//
LOCUS       DATGFG010000597         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_613046, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000597 DATGFG010000000
VERSION     DATGFG010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613046"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="VKQ52_11120"
     CDS             <1..1957
                     /locus_tag="VKQ52_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ87787.1"
                     /translation="SGSVVLTQSTARALFGSQDPLNKVIRRGRGYVVKVTAVIKDLPR
                     NSTFRFDYLTPFSDFAAIDWVKAATTNWSDAYFALYVSLRPGADAARAAAKARGLVRQ
                     YAPASYRTFHREAMLYPMKDWHLYTDFKNGEAVGGLIDYIRMFSIVGVLVLFIACINF
                     MNLSTARSEKRAREVGVRKVIGSSRTGLIVQFLVESVILTFFSFVFALGLVQLALPAF
                     NALAGTAIRVSYDSGIFWLIMLSYVLFTGLLAGSRPAFYLSSFQPVKVLKGAMRVGRA
                     ATLPRKVLVVLQFSCSIALIISTIIVYQQIQYAKDRPRGYDPNRLLVTDVHHDNYRAY
                     KQEVLQSGVVSSVTQSLSPATEIYSHNTIDNWPGRLANEPLSLAMNAVADTDYFRTLG
                     IGFVAGGNFKGNFGVDSLNVILNEAAIRRMRLKDPINQFIGWSPSNAPHRLRIIGVVR
                     DALTRNPFAPAEPTLFVYQPGWTGEISYRLSPTVSTPEALARLKAIYQKYNPDFPFDY
                     SFVDDSYASKFALETLIGRLAGIFAGLAIFISCLGLFGLAAYMAEQRTKEIGIRKVLG
                     ASVAQVLVLLSGDFMVLVGISCVIASPVAYYFLHQWLLGYYYRISIHPFVFVLAAVAA
                     MAITAVTVGFQSVRTALRTPVNSLRSE"
     gene            complement(2000..>2904)
                     /locus_tag="VKQ52_11125"
     CDS             complement(2000..>2904)
                     /locus_tag="VKQ52_11125"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HLZ87788.1"
                     /translation="RQGIAGYQTYDSGFNRHYTDFFPSAALTWNQTPAQQWTLTYSRR
                     IDRPAYQDLNPFEFKLDEYTYKKGNTDLRPQYTNSIGLSYVLKSKLVATLNYSHIKDV
                     FTQLVDTTGRSKAFLIKKNLATQDITSLNISYPWSIGRYSLFANVNTFYSAYNANFGS
                     GRTVNLNVFSANVFVQQGFRLGKDWTADLSGFYTSPAIWQGTFKTHALGNVDIGAQKA
                     IWNKKGLIKLSVSDIFFTQHWTAVSDFAGQYVRSTGHTESRQLKLYFTYKFGNSQVKA
                     ARARKTGAEEESKRVSTQGGGITN"
BASE COUNT          517 a          690 c          943 g          754 t
ORIGIN      
        1 gtccggctcg gtggtgctta cccagtcgac ggccagggcg ctgtttggga gccaggaccc
       61 gttgaacaag gtcatccggc gaggccgcgg atatgtggtc aaggtaacgg ccgtgataaa
      121 ggatctgccg cgcaattcga ctttccgctt cgattatctt actccttttt ccgattttgc
      181 cgccatcgac tgggtcaagg cggcgaccac caattggagc gacgcttatt ttgcgctgta
      241 tgtttcgttg cggccgggcg ccgatgccgc gcgggccgcg gcgaaggcga ggggtctggt
      301 gcggcagtat gcgcccgcgt cctaccggac atttcaccgg gaagcgatgt tgtacccgat
      361 gaaggactgg catctgtata cggattttaa gaacggggag gctgtgggcg gattgatcga
      421 ttatatccgg atgttcagca tcgtcggggt gctggtgctt tttatcgcgt gcatcaattt
      481 tatgaacctt tccacggcca ggagtgagaa gcgggcacgg gaagtgggag tgcggaaggt
      541 gatcgggtct tcgaggaccg ggcttatcgt gcagtttctt gtggagtcgg ttatcctcac
      601 gtttttttcg tttgtatttg cgcttggttt ggtgcagttg gcgctgcccg cgtttaacgc
      661 cctcgcggga acggcgatca gggtttccta cgacagcggg atattctggt tgatcatgct
      721 ttcctatgtt ttgtttaccg gtctactggc ggggagcagg ccggcatttt atctttcctc
      781 tttccagccg gtgaaggtat taaaaggagc catgcgcgtc ggccgggcgg ccacgctgcc
      841 gcgcaaggtg cttgtcgtcc tgcagttttc ctgtagcatc gcgctgatca tcagcaccat
      901 tattgtttat cagcagatac agtatgccaa ggaccgcccg agaggctatg atccgaaccg
      961 gctgctggtg accgatgtgc atcacgataa ctaccgggcg tataagcagg aagtattgca
     1021 gtcgggcgtc gtgagcagcg tgacccaatc gctgagcccg gccaccgaga tctattcgca
     1081 taatactatc gataactggc cgggccgctt ggcgaatgag ccgttgtccc tggcgatgaa
     1141 tgccgtggcg gatactgatt atttccggac cctgggtatc ggttttgtgg cgggcggaaa
     1201 tttcaagggc aattttgggg tggactcgtt aaacgtcatt ctcaacgagg cggcgatccg
     1261 gcggatgcga ttgaaggatc cgatcaacca gtttattggc tggagtccgt ccaatgcgcc
     1321 ccaccggctg cggatcatcg gcgttgtcag ggatgcgttg acgcgtaatc cttttgcgcc
     1381 ggcggagccg accctgtttg tataccagcc gggttggacg ggcgagatat cctatcggct
     1441 gtcgccgacc gtgagtacgc cggaggccct ggcccggcta aaggccatct accagaaata
     1501 caatcccgat tttcctttcg attattcctt tgtggatgat agttatgctt cgaagtttgc
     1561 gctggagacg ctgatcgggc ggctggcagg gatctttgcg gggctggcga tctttatatc
     1621 ctgtcttggt ctttttgggc tggcggcgta tatggccgag cagcggacga aggagatcgg
     1681 catccggaag gtgttgggtg catccgtggc gcaggtgctg gtgctgttgt cgggggattt
     1741 tatggtactg gtggggatca gttgtgtgat cgcgtcgccg gtggcgtatt atttcctgca
     1801 tcagtggttg ttgggatatt attatagaat tagtatccat ccttttgtgt ttgtgctggc
     1861 ggccgtggcg gcgatggcga taactgccgt gacggttggc ttccagtcgg tgaggacggc
     1921 gttgcggacg ccggtgaaca gcctgagatc ggagtaagcc ttctctttcc ggtgactgca
     1981 tcatcgggat gccggatcat cagttggtga taccgccgcc ctgggtgctg acgcgtttac
     2041 tttcttcttc ggctccggtt ttgcgggcgc gggccgcttt gacctgggaa ttcccgaatt
     2101 tataggtgaa atagagcttc agttgccggc tctcggtgtg gccggtggag cggacgtatt
     2161 ggccggcgaa gtcactgacg gccgtccagt gctgggtaaa gaagatatcg cttaccgaga
     2221 gctttatcaa tcctttttta ttccagatgg ctttctgggc gccgatgtcg acgttgccaa
     2281 gggcatgcgt cttgaacgtt ccctgccaga tggcgggcga tgtatagaag ccggagagat
     2341 cggccgtcca gtcctttccg aggcggaagc cctgctggac gaagacattt gcggagaaga
     2401 cgttgagatt cacggtacgg ccgctgccga agttggcatt gtaggccgag tagaaggtgt
     2461 tgacgttggc gaacaggctg taccgtccga tggaccaggg gtagctgata ttgaggctgg
     2521 taatgtcctg ggtcgccagg ttcttcttta tcaggaaggc cttgcttctg ccggtggtat
     2581 ccaccaattg cgtgaacaca tcctttatat gactgtagtt gagggtggcg accaatttcg
     2641 acttcaggac atagctaagg ccgatgctgt tggtgtattg ggggcggaga tcggtgttcc
     2701 ctttcttgta agtgtattca tcgagtttga attcgaaggg attcaggtcc tggtaggcag
     2761 gccggtcgat ccggcggcta taggtaaggg tccactgctg cgccggggtt tggttccagg
     2821 tgagggctgc actggggaaa aaatccgtgt aatggcggtt gaatcccgag tcataggttt
     2881 gatacccggc gatgccctga cggt
//
LOCUS       DATGFG010000598         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_211543, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000598 DATGFG010000000
VERSION     DATGFG010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211543"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VKQ52_11130"
     CDS             complement(<1..417)
                     /locus_tag="VKQ52_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87789.1"
                     /translation="MLILLDCRPLQNAGPESEKSRLILSVTAALSREEGLQWLLLVDH
                     TCPAGIFPGLPGHTLLRQRAFPGRTGWRIWYDWQIPRLARKHKAGLVMLTGGVAAGVL
                     PAPQYLWMPERANPGEGRGHLSLYGGRLAESLRRAGA"
     gene            707..1843
                     /locus_tag="VKQ52_11135"
     CDS             707..1843
                     /locus_tag="VKQ52_11135"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HLZ87790.1"
                     /translation="MAHAQCNCSATVPATPVSYYSSFPSTNAATTTVSFPQFNPSIGT
                     LACVSLNDTVTGVTTTSALNKASSSVTYKFQLTVSNDLEGPPGGDLSISNSYNRTYGP
                     STLARLGFPGDTITYGPDTIFNNEIGYASTSSGTAPYLGNGTVDFTYSLSGGVVSLQG
                     GLNYTAGPTTNYWGAFKLTYYWCPSTTLSTTIQDFTASPTDGSIILQWLTTNQQPNTQ
                     YEIQISTDGKSFYSVGEAEGDASATGTSAKYQYQYNPDPSHLGKLYFRIEEKDPFGKV
                     SYSAVLVVDPTASGKPDAVSYQTFPNPATNSLQVQFNSTQTGRFLVELVGTSGQVIQK
                     KAVTLTGGSSIRLDLDPQPVKGLYFLRTTDLTHEHSYVSKVFIN"
     gene            1967..2677
                     /locus_tag="VKQ52_11140"
     CDS             1967..2677
                     /locus_tag="VKQ52_11140"
                     /inference="COORDINATES: protein motif:HMM:NF025488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HLZ87791.1"
                     /translation="MEYNTTRNHLIMREYGRHIQKMIEYLLTLEDREKRERNAQVVIE
                     LMGFLNPHLKNVEDFRHKLWDHLFLISDFKLDVGSPYPIPTRETLRARPKPLKYPKRY
                     PRFSHLGKNLEIVIDKALAEENPDKRNGFANAVAYYMKLAYNNWHKETVHDDAIQSEL
                     SQLTNGQLEFTNTPFVKSFRPNENREREANGNRTYNDYRNNKRNQKYQQRNNNNNNNR
                     NNDRNNDRGGNKFKKKRF"
     gene            2696..>3021
                     /locus_tag="VKQ52_11145"
     CDS             2696..>3021
                     /locus_tag="VKQ52_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87792.1"
                     /translation="MHSFEVIGGKKLKGEIIPQGAKNEALQIISAVLLTPEKMTISNI
                     PDILDVNLLIDLLVEMGVKVERPQRDTCIFQADNVDTDYLRSEAYRQKSGRLRGSVML
                     AGPMLAR"
BASE COUNT          774 a          894 c          717 g          636 t
ORIGIN      
        1 cgccccggcc cggcgaaggc tttccgccag ccgcccgcca tacaacgaca aatggccccg
       61 gccctccccg gggtttgccc tttcgggcat ccacaggtat tgcggcgccg gcagtacacc
      121 cgcggctacc cctcccgtca gcattaccag gccggccttg tgctttctgg ccagccgcgg
      181 gatctgccag tcataccaga tacgccaccc cgtccgcccc ggaaacgccc gctgacgaag
      241 aagggtatgt cccggcagcc cggggaaaat accggccggg caggtatgat ccaccagcag
      301 cagccattgc aggccctcct cccggctgag ggcggctgtg acagagagga taagacggct
      361 tttttcactt tccggcccag cgttctgcag gggccggcaa tccagcagaa tgagcatacg
      421 caaaggtagg taaggagggg ctattccggc cggtccggtt tttatccaca cagacgcaga
      481 catcaaacta atgacaatca cttacttgaa tatataaaag gatctgcaaa aatgtggttt
      541 ttacggtaca gtcaaatcgg gtagtaatgc cttggcctca ttcttgcagt tgtataattt
      601 tgagtattaa tccgtttaaa tccgatcact attaaaattc gtgcctatga accgtgtgta
      661 cactcctgta tttattatca ccatgttgat agtgaatatc cctcggatgg ctcatgccca
      721 atgtaactgt agtgcgactg ttcccgccac acccgtaagt tactactcct ccttcccatc
      781 caccaacgcc gctaccacca ccgtcagttt ccctcagttc aacccttcga tcggtacact
      841 cgcctgtgtc agcctgaacg atacggtcac gggggtcacg accacttcgg cgcttaataa
      901 agcttccagc tccgttacct ataagttcca gctcaccgta tcgaatgacc tggaaggccc
      961 tcccggcggc gacctgtcca tctccaacag ctataaccgc acctatgggc cttcgaccct
     1021 ggcccgcctc ggcttccctg gagatacgat cacttacgga ccggatacca ttttcaataa
     1081 cgagatcggc tatgcctcca ccagcagcgg tacggcgccc tacctcggga acggaacggt
     1141 ggattttacg tatagcctta gcggtggggt cgtttccctc caggggggcc tgaactacac
     1201 cgcgggccca accacgaact actggggagc attcaagctc acctattact ggtgcccctc
     1261 gacaactctt tccaccacca tccaggattt tacggcctct cccaccgatg ggtccatcat
     1321 cctgcaatgg ttaaccacaa accaacaacc caatacccaa tatgaaatcc aaatcagtac
     1381 agacggcaaa agtttctatt ccgtcggaga ggcagaaggc gacgcttctg caacaggtac
     1441 ttctgcaaag taccaatatc aatacaaccc tgatccgtct catctgggca agctctattt
     1501 ccggatcgaa gagaaggatc ctttcggaaa agtaagctat tcggccgtcc ttgtcgtaga
     1561 tcccactgcc tccggtaagc cggatgccgt cagctaccag accttcccca accccgccac
     1621 gaatagtctc caggtacagt tcaacagcac ccagacaggc aggttcctgg tagagctcgt
     1681 agggacgtcg ggacaggtca tacagaaaaa agcggtgacg ttgacaggcg gcagctcgat
     1741 caggctggat ctcgatcccc agccggtgaa agggctttat ttcctgcgga cgaccgacct
     1801 cacccacgaa catagctatg taagcaaggt ttttatcaat tagcatttcc tctgaaaaac
     1861 cataaaatcc aattagcctg cgagaagcct ccaccggagg cttttccttt tgcggagttt
     1921 gcaggctggg ctttttcatg tagatttgtt tccttaaatc gagtgtatgg aatataatac
     1981 cacgcggaat caccttataa tgcgtgaata cggccggcat atccagaaga tgatcgaata
     2041 tcttctgacg ctggaggaca gggaaaaacg ggagcgtaac gcacaggtgg ttatcgagct
     2101 gatgggcttt ctcaatccgc atctgaagaa cgtagaagat tttcgccata aattatggga
     2161 ccatctcttc ctcatctccg acttcaaact ggacgtcggt tccccctatc ccatccctac
     2221 ccgggagacg ctgcgggccc gtccgaagcc gctcaaatac cccaagcgct acccgagatt
     2281 ctcccacttg ggaaagaacc tcgagatcgt catcgacaag gccctcgcgg aagagaaccc
     2341 cgacaagcgc aatggtttcg ccaatgccgt ggcctattat atgaagcttg cctacaacaa
     2401 ctggcataag gaaaccgtcc atgatgacgc catccagagc gaactttcgc agctgaccaa
     2461 cgggcagctg gaattcacca atacgccctt tgtcaagagc ttccgtccca acgagaaccg
     2521 cgaaagagaa gccaacggca accgcaccta taatgactac cgtaataaca agcggaacca
     2581 gaagtatcag caacggaaca acaataacaa caacaaccgc aataacgacc ggaataacga
     2641 ccggggcggg aataaattca aaaagaaacg attctgacgg atcaaatcaa cgcgcgtgca
     2701 ttcatttgaa gtcatcggcg gtaaaaaact taagggagag attattccac aaggcgctaa
     2761 gaacgaagct ttacagatca tcagcgccgt cctgctgacg ccggagaaaa tgaccatctc
     2821 caacatccct gatattctcg atgtaaatct gctgatcgac ctgctggtcg agatgggtgt
     2881 caaggtcgaa agaccacagc gggatacctg tatcttccag gccgacaacg tggatacgga
     2941 ctatctgcga agcgaagcct atcgccagaa aagcggccgg ctccgcggat cggtcatgct
     3001 ggcgggtccc atgctggccc g
//
LOCUS       DATGFG010000599         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_269752, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000599 DATGFG010000000
VERSION     DATGFG010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269752"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VKQ52_11150"
     CDS             complement(<1..566)
                     /locus_tag="VKQ52_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLZ87793.1"
                     /translation="MRYCFFLGLFFWGLAVSAQDGSWSCYGQNAGGSRYSALRQINSR
                     NVAQLKVAWTCRTGELAKYEGTYALEKAAFETTPILIGRTLYLSTPSDRVIAVDAATG
                     QQRWVYDPNVNLHADHSEISNRGVAYWSGGRVFIGTIDGRLIALDAATGRPAASFGKD
                     GIVDLRAGVGEDVAETSPPVVTGDLVIVG"
     gene            765..>2827
                     /gene="ppk1"
                     /locus_tag="VKQ52_11155"
     CDS             765..>2827
                     /gene="ppk1"
                     /locus_tag="VKQ52_11155"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HLZ87794.1"
                     /translation="MRRKTIPRDISWLSFNGRVLQEAADPTVPLRERIRFLGIFSNNM
                     DEFFRVRVATLRRMSKYSGRKSRLNMHLEASPDKILNDIQAIVLEQGDEFDAIWGGIR
                     KEMENEKIFLVTEQDLDPTQQQFIQTYFEEEVRTNTIPLMIESIPTFPFLRDKSLYMG
                     VVLSRRDGSMKRKYAIIEIPSRVLGRFVILPSPSMDEHHIMLLEDIIRFNLRNIFAYF
                     GYDSYDSWVFKVTRDAEIDIDNDISTTLIQKIEKGLKNRRKGKPVRFVYDEQMDKGLL
                     DYLLRRLNLSKKSGNLIPGGRIHNFRHFMDFPDVFPKQGHRKKPFTHPALQTTPRVTD
                     VVLEKDVMLHFPYHSFNPLIDMLREAAIDPNVTTIKITGYRLASNSKIINALINAVRN
                     GKHVTVMLELRARFDEEANLEWKERLEDEGVKVLIGIPNLKVHAKICLIKKRINNFTI
                     HYGFVSTGNLNEKTARVYGDHCLLTSDRHVMADVNRIFNYLENYKDGPAPLKACKTLI
                     PCPTNLRHELVKMIARETKLAREKKPAAITLKMNSLSDEDLIEKLSEAAKAGVRIQMV
                     IRGIFCMFSENSKFLIPVKAISIIDEYLEHARVFIFHNGGKEKTYISSADWMVRNLDH
                     RVEATCPILDEGIARELKEILNIQLSDNVKARWLDNELLNKYKRDHTENKVRSQIEIY
                     NYLYQ"
BASE COUNT          718 a          879 c          673 g          557 t
ORIGIN      
        1 cccacgatga caagatcacc cgtcaccacc ggcggcgagg tctcggcaac atcctccccc
       61 accccggcac ggaggtcgac gatcccgtcc ttgccaaaag acgcggccgg ccggcccgtt
      121 gcggcatcca gcgcgatcag cctgccgtcg atagtcccga tgaaaaccct gccgcccgac
      181 cagtacgcga ctccccggtt actgatctcc gagtgatcag cgtgcaggtt cacattcgga
      241 tcatagaccc atcgttgttg ccccgtcgca gcatccacgg cgatcacccg gtcgctgggc
      301 gtggagagat aaagcgtccg cccgatcagg atcggcgtcg tctcgaatgc cgccttttcc
      361 agcgcatacg tgccctcata tttggccagt tcgcccgtcc ggcaggtcca cgccaccttt
      421 agctgcgcca cattgcggct gtttatctgc cgcagggcgg aataccggct gccgcccgca
      481 ttctgtccgt agcaggacca ggagccatcc tgcgccgata cggccagccc ccaaaaaaat
      541 aacccgagga aaaaacaata tcgcatagct gtaattttgt ctcaaaccta tcggaataac
      601 caaacgtgtc cgattttctt tgaccaaaag accgccttca ttgacgtgcg gacaaagggc
      661 ataattctct tcatgtgttt aattaataat tacttaattt acttatccct tttgcattgg
      721 gtactttgca aaaaaagggg ataatgacag aaacaaccgg ttcaatgaga agaaaaacca
      781 tcccacgcga catctcctgg ctttccttta acggccgggt gctgcaggaa gcagcagacc
      841 ctactgtgcc attgcgtgag cgcatccgtt tcctcggcat tttttccaat aacatggacg
      901 agttcttccg ggtccgcgtc gccaccctgc gacgcatgag caaatacagc ggcagaaagt
      961 cccggctcaa tatgcacctc gaagcatctc ctgacaagat cctgaacgat atccaggcca
     1021 tcgtcctgga acaaggcgat gagttcgacg ccatctgggg cggcatccgc aaggagatgg
     1081 agaacgaaaa gatcttcctg gtcaccgaac aggacctcga ccctacccag cagcaattta
     1141 tacaaactta ttttgaagag gaagtacgca ccaacactat cccgttgatg atcgagagca
     1201 tccccacctt ccccttcctg cgcgacaaat ccctctacat gggcgtcgtg ctctcccgcc
     1261 gcgacggcag catgaaacgg aaatacgcca tcatcgagat ccccagccgg gtcctcggcc
     1321 gtttcgtcat cctgccctcc cccagcatgg acgaacacca tatcatgctg ctcgaggata
     1381 tcatccgctt caacctgcgc aatatcttcg cctacttcgg ctatgatagc tacgactcct
     1441 gggtcttcaa ggtcacccgc gacgcggaga tcgatatcga caacgatatc tccaccaccc
     1501 tcatccagaa gatcgaaaaa ggactgaaga accgccgcaa gggtaaaccg gtgcgcttcg
     1561 tctacgacga acagatggac aagggcctgc tggactacct gctcagacgg ctcaacctct
     1621 ccaagaagag cggtaacctc atccccggcg gccgcatcca taacttccgg cattttatgg
     1681 acttcccgga tgtgttccca aaacaggggc atcggaaaaa accctttacc catcccgctc
     1741 tccaaaccac gccccgggtc accgacgtgg tcctcgaaaa ggatgtgatg ctccacttcc
     1801 cctaccattc cttcaacccc ctgatcgata tgctgcgcga agccgccatc gatccaaacg
     1861 tcaccaccat caagatcacc ggctaccggc tggcctccaa ttcgaagatc atcaacgcgc
     1921 tgataaatgc cgtccgcaac ggcaaacacg tcaccgtgat gctcgagctc cgcgcccggt
     1981 tcgacgagga agccaacctc gaatggaagg aacggctcga ggacgagggt gtcaaggtcc
     2041 tcatcgggat ccccaacctg aaggtgcacg cgaagatctg tctcatcaag aagcggatca
     2101 acaatttcac catccactac ggctttgtca gcaccggcaa cctgaacgaa aagacggcac
     2161 gggtatatgg cgaccactgc ctgctcacct ccgatcgcca cgtgatggcc gacgtcaacc
     2221 gcatctttaa ttacctcgag aactataagg atggaccggc cccgctaaag gcctgcaaga
     2281 ccctcatccc ctgccctacc aacctgcggc acgaactggt aaagatgatc gcccgggaga
     2341 ccaaattggc cagggaaaag aaaccggcgg ccatcaccct caagatgaac tcgctgtcgg
     2401 acgaagatct tatcgagaaa ttgtcagagg cggcaaaagc gggagtcagg atccagatgg
     2461 tcatccgggg gatattctgc atgttctccg agaacagcaa gttcctgatc cccgtaaaag
     2521 ccatcagcat tatcgatgaa tacctggaac atgcccgcgt gtttatcttc cataatggcg
     2581 gaaaagaaaa aacttacata tcttcggccg actggatggt ccgtaacctc gatcaccggg
     2641 tggaggcaac ctgtcccatc ctcgatgagg gcatcgcccg ggaattgaaa gagatactga
     2701 acatccagct gtcggacaac gtcaaggcgc gctggctcga caacgaattg ctgaataagt
     2761 ataaacgcga tcataccgag aacaaggtgc gatcgcagat cgagatctat aattatttgt
     2821 atcaaaa
//
LOCUS       DATGFG010000600         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_343490, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000600 DATGFG010000000
VERSION     DATGFG010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343490"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..299
                     /locus_tag="VKQ52_11160"
     CDS             48..299
                     /locus_tag="VKQ52_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87795.1"
                     /translation="MKPRITTTDPQASASPVKSHFSLTKYMLFFVLLIAVVLNGLTGY
                     HLFQHSNYDLSSLMIIASYLSIVMSIYVLTVSRRKPAIH"
     gene            589..2196
                     /locus_tag="VKQ52_11165"
     CDS             589..2196
                     /locus_tag="VKQ52_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87796.1"
                     /translation="MKRSVFRWFRDISIAKKLYFTVGIMALLIAIELIVLFFSIGTLS
                     SVRAYVGGEGLWSKAQKDGLYQMLKYARTHDEKDYDKFQEYLKVNMGDHKARIELGNP
                     NPNLDIVRQGFIEGRNHPEDVDGMIKLFRRFHSNAYIHRAIVAWTKADATIMDFVDLG
                     RRLHTVINSPSSSPQQINALLDEIDPLTNRLTPQEDEFSFALGEGSRWLEGVVLRLLF
                     IVALTVECTGLILAITVSRGIQKGLQEILLSARAVKKGDFSRKARSYSADEIGTLANN
                     FNSMAEELQKLELENREVNNSLEKKVYQRTQEIESKNKELEQFAYVASHDLQEPLRTI
                     SGFVDLLKKEYRDKLDGNGHIYLNYLAQASDRMKTLIKDLLDYSRIGREKEAMPVDCN
                     LLLDEVLADLGKSIRESGAHVISGGLPIIRAYPTELKLLFQNLIANSIKFRHPERLPE
                     VSVSVTQQNGKWQFAVSDNGIGIEKQFLERIFIIFQRLHSRTQYEGSGIGLAHCKKIV
                     EFHEGRIWADAEPGKGSRFFFTISKDL"
     gene            complement(2279..2512)
                     /locus_tag="VKQ52_11170"
     CDS             complement(2279..2512)
                     /locus_tag="VKQ52_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87797.1"
                     /translation="MKRNGIFPLLLVLFVLAVVVMARCGGPEPRKMAVDSTSPMGPAP
                     DNNSANNPSLADTNYEKDRTRPITDTMKKDSGR"
     gene            2646..3584
                     /locus_tag="VKQ52_11175"
     CDS             2646..3584
                     /locus_tag="VKQ52_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HLZ87798.1"
                     /translation="MDFIDYYKVLGVNKNATQDEIRSAYRKLARKHHPDLNPNDKEAN
                     KKFQQINEANEVLSDPEKRKKYDQYGKDWQHAEAFEKAQQEAGRQGGRSRGTGGFEGF
                     GGTTFGGAEDFEPGEGGEFSDFFESLFGGGGRRGGNSKARFRGQDYQAELQISLTEAA
                     TTHQRVLTVNGKNIRITIPAGVEDGQIIKLKGHGAPGSNGGPDGDLYITFSITEDPRW
                     TREGDHLHMKAPLDLYTAMLGGEITVETLTGMVKLKIKPETPNETTMRLKGKGFPVYK
                     KEGQHGDLFVSLEIKLPTHLTEKEKELFRQLKDLQH"
BASE COUNT          952 a         1219 c          780 g          674 t
ORIGIN      
        1 gtctataatt taaacaacaa aaattgtcac ctctaaaaat gaaaaatatg aaaccgagaa
       61 taacaaccac cgacccgcaa gcttccgcca gccccgttaa gagccatttt tccctgacga
      121 aatacatgct cttcttcgtc ctgctgatcg ccgttgtact caacgggctt accgggtacc
      181 acctctttca gcattcgaac tatgatctca gcagcctgat gatcatcgcg tcctatctgt
      241 cgatcgtcat gagcatctac gtgctcaccg tgagtcgccg caaaccagcg atacactaat
      301 tccccatcat acatacaacg acccggccgc cgcaaagtga ccgggtcttt ttctgtccgc
      361 cacccgctca cggtcctccc tctctccact ctctcccggc tccgctttct ctgccgttcc
      421 cctctctccc ctctgccccc cggcccgcgc tctcactgtc attccccgct ccacacggtc
      481 ctccctctct ccgcccgccc ctcttttttt gctattcccc cactcttttc ttactttgta
      541 ccaccctggc acacctgaca cacctggcgc ttaccaaaac cttcaagtat gaaaagatcc
      601 gtctttcgat ggttcaggga tatctccatt gccaaaaagc tctatttcac agtcggcatc
      661 atggccctgc tcatcgcaat cgaactgatc gtcctgttct tctccatcgg aaccctctcc
      721 tccgttcgcg cttatgtcgg cggcgaagga ctctggtcca aggcacagaa agacggcctc
      781 taccaaatgc tcaaatacgc ccgcacccat gatgaaaaag actacgacaa attccaggaa
      841 tacctcaaag tcaacatggg cgaccacaaa gcccgtatcg aactcggcaa ccccaatccc
      901 aacctcgaca tcgtccgcca ggggttcata gagggccgta accaccccga agacgtcgac
      961 ggcatgataa aactcttccg ccgcttccat agcaacgcct atatccaccg cgctatcgtc
     1021 gcatggacaa aggccgacgc caccatcatg gacttcgtcg atctcggccg ccgcctccac
     1081 accgtgatca attccccctc cagctctccc caacagataa acgccctcct cgatgagatc
     1141 gatccgctga ccaaccgcct caccccacag gaagacgaat tctcatttgc tctcggcgaa
     1201 ggctcccgct ggctcgaggg ggtcgtactg cgactgctgt tcatcgtggc gctgaccgtc
     1261 gaatgcaccg gcctcatcct ggccatcacc gtcagccgcg gcatccagaa aggcctgcag
     1321 gagatcctcc tctccgcccg ggcggtaaag aaaggtgact tcagccgcaa ggcccggtcc
     1381 tattccgccg acgagatcgg caccctggcc aataacttca actccatggc cgaagaatta
     1441 caaaaactgg aactggagaa ccgggaggta aacaactccc tggaaaaaaa ggtctatcag
     1501 cggacccagg aaatagaaag caagaacaag gaactggaac aattcgccta tgttgcttcg
     1561 cacgatctcc aggagccgct ccgcaccatc tcaggattcg tcgatctcct caagaaagaa
     1621 taccgcgaca aactcgacgg caacgggcat atctatctga attatctcgc ccaggcatcc
     1681 gaccggatga agacccttat aaaagacctg ctcgattact cccgcatcgg ccgcgaaaaa
     1741 gaggccatgc ccgtcgactg caacctgctc ctcgatgaag tcctcgccga tctgggtaag
     1801 tccatccgtg aatccggcgc ccacgtgatc tccggcggcc tccccatcat ccgcgcctat
     1861 cccaccgaac taaaattgct gtttcaaaac ctcatcgcca actccatcaa attccgccat
     1921 cccgaacgct tacccgaagt atcggtctcg gtaacccagc agaacgggaa atggcaattc
     1981 gccgtcagcg acaacggcat cggcatagaa aaacaattcc tggaaaggat cttcatcatc
     2041 ttccagcgcc tgcactcccg cacccagtac gaaggctcgg gcatcgggct ggcgcactgc
     2101 aaaaagatcg tggaatttca cgaaggccgc atctgggcgg acgccgaacc gggaaaagga
     2161 agccgcttct tcttcaccat cagcaaggac ctctgacccc ggcaccccga atacctctat
     2221 ctccaaaaat ctctgacccc ggcacctctc cccacaaata ccctgcgccc cggcccctct
     2281 aacgcccact atcctttttc atcgtatcgg ttatcggccg cgtccggtcc ttttcataat
     2341 tcgtatcggc aagagaagga ttgttggcgc tgttattatc cggcgccggc cccatcgggg
     2401 aggtggaatc gacagccatc ttccggggct ccggacctcc acacctggcc atcaccacaa
     2461 ccgcaagcac aaacaggacc aataacaggg ggaaaatacc gtttcgcttc ataactgtct
     2521 tttactcagc ggtaaaaaaa ccatgccatc acccctcgac caactccccc aaaaagcacc
     2581 ctaaaactcc caaccaacca caacctgaaa aatctgttaa ctttgtcaaa aaggatactc
     2641 atacaatgga tttcatcgac tactataagg tcttgggagt gaacaaaaat gccacgcagg
     2701 acgagatccg ctccgcctac cggaaattgg cccgcaaaca ccatcccgac ctcaacccga
     2761 acgataagga agcaaacaag aaattccagc agataaacga ggcgaacgaa gtactcagcg
     2821 accccgagaa aagaaagaaa tacgaccagt acggtaaaga ctggcaacac gcggaagcat
     2881 tcgaaaaggc ccagcaggaa gccgggagac agggcggccg ctccaggggt acgggcggct
     2941 tcgaaggctt cggaggcacc accttcggcg gcgcggaaga cttcgaaccc ggcgaaggcg
     3001 gggaattctc cgacttcttc gaatccctct tcggcggcgg tggccggcgc ggcggcaaca
     3061 gcaaggcgag gttccgcggc caggattacc aggccgaatt acaaataagc ctgaccgaag
     3121 cggcaaccac ccaccagcgc gtactcaccg tcaacggtaa gaacatccgc atcaccattc
     3181 ccgccggggt agaggacggc cagatcatca aactgaaagg acacggcgcc cccggctcca
     3241 acggcggacc cgacggcgac ctctatatca ccttctccat caccgaagac ccccgctgga
     3301 caagagaagg cgaccatctc cacatgaagg cccccctcga tctctacacc gccatgctcg
     3361 gcggcgagat cacggtcgaa accctcaccg gtatggtcaa actaaaaata aaacccgaga
     3421 ctccaaacga aaccaccatg cggctaaaag gaaaaggctt ccccgtttat aaaaaggaag
     3481 gtcagcacgg cgatcttttt gtcagcctgg agataaaact ccccacccat ctcaccgaaa
     3541 aggaaaaaga actcttccgg caactaaaag atctgcaaca ctaaaaaagc ccaaaccaat
     3601 acggtacaac cgacaggccc tccca
//
LOCUS       DATGFG010000601         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1152098, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000601 DATGFG010000000
VERSION     DATGFG010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152098"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2023
                     /locus_tag="VKQ52_11180"
     CDS             <1..2023
                     /locus_tag="VKQ52_11180"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87799.1"
                     /translation="PMRAIIFFLLIGAGLPILLPAQSPASTTAGSTWTIQADSIDPAN
                     YYGVTVANGMVGLVSSPEPFRAKDVVLNGAFDLYGRGRVSNILKTFNFVNGYLDIDGR
                     RIDNFKQVTHLRQTLDMQHACLTTTFDYGDKASVSYTWYSLRQLPYTALVDVTITAHK
                     DIEIVPASVMEAPDMLKDVENYYNEIDRPHVTISLLTSTAKSPTGKLLMAASNSILFD
                     EAHGTEPAIIHEMWDNNMHLIKFRKKLQTGATYHFAVVGSTLTSAHNDDPLNEAERLT
                     LFATLQGSKQLIKAHEQAWDQLWQSDILIDGDDALQRDIRLMLYHLYSFAREGTAYSL
                     SPMGLSGLGYNGHTFWDTEIWMYPALLLLQPKIAASLMEYRYRRLPAAMQNAADHGYK
                     GAMFPWESAASGNEETPVWALSGPFEHHITADVGIAAWNYYCVTRDKTWLKEKGYPLL
                     QATADFWTSRVERNSPGHYDLRNVVAADEWAENVDNNAYTNAAARANLQYATEAARIL
                     GLTPDPDWMNVRNNIPILRFPDGTTREHAAYNGESIKQADVNLLSYPLKEITTPAEIR
                     RDLDYYEPRVGEGPAMTQAIFAILYERLGLPDKALKVLKQGYTPNLRPPFGVIAETAT
                     GNNPYFATGAGGVLQAMLNGFGGLDITGNGIVQLPTNSPPAGTPSGSPA"
     gene            2173..>2499
                     /locus_tag="VKQ52_11185"
     CDS             2173..>2499
                     /locus_tag="VKQ52_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87800.1"
                     /translation="MKFSLTLLKATLRNLYFLVFLNGFLLAALLCFKMDAAYENGLFT
                     SIQSSINLQIDSDDNADSIVVKSMNICYYLMRPRATTFAGKASAEMGPEAGLFRSTAV
                     DLMTASG"
BASE COUNT          605 a          910 c          552 g          432 t
ORIGIN      
        1 acccatgcgt gccatcatct tcttcctcct catcggtgcc ggcctcccca tcctcctgcc
       61 ggcgcaatcc ccggcatcca ccaccgccgg ctccacctgg accatccagg ccgacagcat
      121 cgaccctgcc aactactatg gcgtcaccgt cgcaaatggc atggtaggcc tcgtctcctc
      181 cccggaaccc ttccgcgcaa aagacgtcgt cctcaacgga gccttcgacc tctatggccg
      241 cggccgcgtc agcaacatcc tgaagacgtt caacttcgtc aacggctacc tcgacatcga
      301 cggccgccgc atcgacaact ttaaacaggt tacccatctc cggcagaccc tcgacatgca
      361 acacgcctgc ctcaccacca ccttcgacta cggcgacaag gcaagcgtca gctatacctg
      421 gtactccctc cggcaattac cctatacggc cctggtagac gtcaccatca ccgcccataa
      481 agacatcgag atcgtccctg ccagcgtcat ggaggcgccc gatatgctga aagacgtcga
      541 aaactattac aacgagatcg accggcccca tgtcaccata tccctgctga cctccacggc
      601 caaaagcccc accgggaaac tgctcatggc cgcctccaac agcatcctct tcgacgaggc
      661 ccatggcacg gaacccgcca tcatccatga gatgtgggac aacaatatgc atttgataaa
      721 attcaggaaa aagctgcaaa caggcgccac ctaccacttc gccgtcgtcg gctccaccct
      781 cacctcggcc cacaacgacg atcccctcaa cgaagccgaa cgcctcaccc tcttcgccac
      841 cctccaggga agcaaacaac tgataaaagc gcacgaacag gcctgggatc aactgtggca
      901 aagcgatatc ctcatcgacg gcgacgacgc cctgcaacgc gatatccggc tcatgctcta
      961 tcacctgtac tcctttgccc gcgaaggcac ggcctacagc ctctccccca tgggcctgag
     1021 cggcctcggc tacaacggtc acaccttttg ggacaccgag atctggatgt accccgccct
     1081 gctcctgctc cagccaaaga tcgccgcctc cctgatggaa taccgctacc ggcgcctccc
     1141 ggccgccatg cagaacgccg ccgatcacgg ctataaaggc gcgatgttcc cctgggaaag
     1201 cgcggccagc ggcaacgaag aaacccccgt ctgggccctc agcggcccct tcgaacacca
     1261 tatcaccgcc gacgtcggca tcgccgcctg gaactactat tgcgtcaccc gcgacaagac
     1321 ctggctaaag gaaaaaggct acccgctcct gcaggccacc gccgacttct ggacaagccg
     1381 ggtcgaaagg aacagcccgg gccattacga tctccgcaac gtcgtagccg ccgacgaatg
     1441 ggccgagaac gtcgacaaca acgcctatac gaacgccgcc gcccgggcca acctccaata
     1501 cgccacggaa gccgcccgca tcctgggcct gacgcccgac cccgactgga tgaacgtcag
     1561 gaacaatatc cccatcctcc gcttccctga cggcaccacc cgcgaacacg ccgcctataa
     1621 tggtgaatcc ataaaacagg ccgacgtcaa cctcctcagc tacccgctga aagaaattac
     1681 tacccccgcg gagatccgcc gcgacctcga ttactacgaa ccccgggtgg gcgaaggacc
     1741 tgccatgacc caggccatct tcgccatcct ctacgaacgc ctcggactcc ccgacaaggc
     1801 cctcaaagtc ctcaaacagg gctatacgcc caacctgcgc ccacccttcg gagtgatcgc
     1861 agagaccgca acgggcaata acccctattt cgcaaccggc gccggcggcg tcctccaggc
     1921 catgctcaac ggattcggcg gcctggacat aacaggcaac ggtatcgtac aattgcccac
     1981 caactcccct ccggctggca ctccctcagg atcaccggcg taggtataaa cagaaacact
     2041 tataccacaa agtgatcata acagggtata ccccaataaa taggacattc ttgtaagggg
     2101 atacaataaa agtttctttt aacgtttagt aaaaggaact atccaatatc gccaaaaagc
     2161 ctgtcgccga ctatgaagtt ttcgcttacc ctcctaaaag caaccctgag aaatttgtat
     2221 ttccttgtat ttctcaacgg attcctactg gccgccctcc tttgctttaa aatggacgcc
     2281 gcgtacgaga acggactttt cacctccatc caatccagca tcaacctaca gatcgattcc
     2341 gacgataacg cagactccat cgtggtaaaa tccatgaata tctgctacta tctgatgagg
     2401 ccccgcgcca ccaccttcgc cggcaaagca tccgccgaaa tgggacccga agccggcctc
     2461 tttcgctcca ccgcggtaga cctcatgacc gccagcggc
//
LOCUS       DATGFG010000602         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1487487, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000602 DATGFG010000000
VERSION     DATGFG010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487487"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VKQ52_11190"
     CDS             complement(<1..183)
                     /locus_tag="VKQ52_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLZ87801.1"
                     /translation="MRIGELSKRSGFTRDTIRFYEKKGLIRLPDAGRDRYQFKDYPDS
                     VLKRLLAIRQIKEYGFT"
     gene            279..752
                     /locus_tag="VKQ52_11195"
     CDS             279..752
                     /locus_tag="VKQ52_11195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HLZ87802.1"
                     /translation="MEKMPVSSYIVRFNDCDPLGHLNNARYIDYFLNAREDHLREQYG
                     IDLREWASRGIVFVVSHHDIRYLRPVTYNERVDIQSALIAWGESWLLVEMLMFDAARQ
                     LKAIMWTRFARIDPRTGKRAAHPEELLEWAKETLVEGIEVEKGLGGRVEKLRVAG"
     gene            825..2234
                     /gene="leuC"
                     /locus_tag="VKQ52_11200"
     CDS             825..2234
                     /gene="leuC"
                     /locus_tag="VKQ52_11200"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HLZ87803.1"
                     /translation="MSKLPSTLFDKVWDTHVVRKIEGGPDVLFIDRHFIHEVTSPVAF
                     LGLESRHLKVMFPEKTFATADHNTPTINQHLPVQDALSANQLKALEVNAAKYGISHWG
                     LGNPKNGIVHVVGPENGITLPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLSSQC
                     IMQPKPKKMRISVDGVLGKGITPKDVVLYVISRLTAAGATGYFVEFAGEVFRSMSMEG
                     RMTVCNMSIEMGARGGMIAPDETTFAYIRGREKAPKGAAWDKALAYWKTLQTEEGAVF
                     DKEYHYDAADIEPMITYGTNPGMGIGITRHIPLAQAAGGSKASYEKSLDYMGFHEDEA
                     MLGKKVDYVFIGSCTNGRIEDFRAFASIVKNRKKADHVTAWIVPGSHIVEQQIREEGI
                     LDILTAAGFQLRQPGCSACLAMNDDKIPAGKYAVSTSNRNFEGRQGPGARTMLASPLV
                     AAAAAVTGVVTDPRTLLAD"
BASE COUNT          520 a          547 c          704 g          523 t
ORIGIN      
        1 ggtaaagcca tattctttta tctggcggat ggccaggagg cgtttgagga cggagtcggg
       61 gtagtctttg aactggtacc gatcgcgtcc ggcgtccggg aggcggataa ggcctttttt
      121 ctcgtagaaa cggatggtat ccctggtgaa acccgatctt tttgacagtt cgccgatgcg
      181 catatgggtg tttttgggtg gttcggaaat tttttcgagg ccgctgatga ctgtggagta
      241 tactccagca tctattttcc ctaccaaatt acgcaattat ggaaaaaatg ccggtttcgt
      301 cttatatcgt ccgattcaat gactgtgatc cgcttggcca tctgaacaat gcgcggtata
      361 tcgattattt tctgaacgcg agggaggatc atttgcggga gcagtatggg atcgatcttc
      421 gggagtgggc ttcgaggggg atcgtttttg ttgtcagtca tcatgatatc cggtatctgc
      481 ggccggtgac ctataatgag cgggtcgata tccagtctgc gctcatcgcg tggggggaga
      541 gctggttatt ggtggagatg cttatgtttg acgcggcgcg gcagttaaag gcgatcatgt
      601 ggacccgctt tgcgcggatc gacccgcgga cggggaagcg ggcggcgcac ccggaggaat
      661 tgctggaatg ggcgaaggaa acgctggtgg agggtatcga ggtggagaag gggcttgggg
      721 gcagggtgga gaagctgcgg gttgcgggat agaaaaaatt cattataaaa gctcattata
      781 tggatatttt ttaagaattt tgcagcttgt ttaaattata agaaatgagc aagctgccat
      841 ctacattatt tgacaaagta tgggacacgc atgtggtgag gaagatcgag ggtggtcccg
      901 atgtactttt tatcgatcgc catttcatcc atgaggtgac cagtcctgtg gccttcctgg
      961 ggctcgagag ccgccacctg aaggtgatgt tccctgagaa gacctttgct acagccgatc
     1021 ataatacacc taccatcaac cagcatcttc ccgtacagga cgcgctgtcg gccaaccagt
     1081 tgaaggcgct ggaagtgaat gccgccaaat atggcatctc gcattggggg ctgggcaacc
     1141 cgaagaacgg catcgtgcat gtcgtcggac cggagaacgg gatcaccctt cccgggatga
     1201 ccatcgtctg cggcgacagt catacatcca cacatggggc cttcggcgcc atcgcttttg
     1261 ggatcggcac ctccgaagtg gagatggtgt tgtcctccca gtgtatcatg caacccaagc
     1321 caaagaaaat gcggatcagt gtcgatgggg tattgggtaa gggcatcacg ccgaaggatg
     1381 tggtcctcta tgtcatttcg cggcttacgg ctgccggcgc tacgggatat tttgtcgagt
     1441 ttgccggtga ggtattccgg agcatgagca tggaagggcg gatgacggtt tgcaatatga
     1501 gcattgaaat gggcgcccgt ggcggcatga tcgccccgga tgagacgacc tttgcctata
     1561 tcaggggccg ggaaaaggct cccaagggag cggcctggga caaggccctg gcctactgga
     1621 agaccctgca gacggaagaa ggggccgtct tcgataagga atatcattac gatgccgccg
     1681 atatcgagcc catgatcacc tatggcacca accccggtat gggtatcggc atcacccggc
     1741 atatccccct tgcgcaggcg gcaggcggca gcaaggccag ctacgaaaaa tcccttgact
     1801 atatgggttt ccatgaggat gaggccatgc tgggcaagaa agtagattat gtgttcatcg
     1861 ggagctgcac caacgggcgg atcgaggact tccgcgcctt tgcctccatt gtaaagaacc
     1921 ggaaaaaggc cgatcatgtc acggcgtgga tcgtccccgg cagtcatatc gtcgagcagc
     1981 agatccggga agaagggata ctggatatcc tgaccgctgc cggattccag ctgcgtcagc
     2041 ccggatgctc cgcctgtctg gctatgaacg atgacaagat accggccggg aaatatgctg
     2101 tcagcaccag caaccgcaat ttcgaaggaa gacaggggcc cggcgccagg acgatgctgg
     2161 caagcccgct ggtggccgcg gccgctgccg tgacgggcgt tgtaaccgac ccccggacct
     2221 tactcgcaga ctagatttgt ttcacctcaa caaccgaaaa gtagtatggc atacgataaa
     2281 tttactgttt taag
//
LOCUS       DATGFG010000603         6484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_266386, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000603 DATGFG010000000
VERSION     DATGFG010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266386"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VKQ52_11205"
     CDS             complement(<1..895)
                     /locus_tag="VKQ52_11205"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HLZ87804.1"
                     /translation="MPLHGDDGYPGDDRYPGDRKYSFAEVLRQIESKGYTVGYPSRLM
                     EQIPRVAVHLPLDSSLDKALKACLAGLNIRYVIEDKTVFFGYKEGAGEGSAIYADLEG
                     VVLDCESQPLPGATVSRTGSNRTVLTDSAGRFRLPVRAFTSEVTVSHVGYADRELALS
                     NRQPNIVVMANVVQELDGVEVSAYSPQWPQESTTASYAWRSAEMFERIDGVSGVLSNL
                     EGRLPGVSIRQYNGVPGSAYEVLLGGKHSIQQGNEPLVVVDGVPVASGVFLPPIGSGS
                     AQGPNGASALNGIPAGDIRKIE"
     gene            complement(1024..2265)
                     /locus_tag="VKQ52_11210"
     CDS             complement(1024..2265)
                     /locus_tag="VKQ52_11210"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HLZ87805.1"
                     /translation="MSSNNHHIIQLLVKRQRGEALTEEEMDQLVKWLETSGFDRPLTH
                     LSTDEQWMAEVLADPRGISEDQVSLNAYVPERWGRRWLRSGVAAVFLILFACGAWLFW
                     RGWERMHPPAADAPHTGRDIPDDAGVVFIGSDDRATRVDTLSVETTFDREGDYRLRKI
                     DSNGMAYEKQGIRARPAGEKQRISAQSPGAAGGRTLLRLVIGRRRRQFHLRLPDGTNV
                     WLAAGSRFSFPSEGREATAPFFLEGEAFFDVVHDPGKPFHLQTPRGEDIQVLGTVFNV
                     EAFKGSQETRVSLFSGLVKLMAVDYDTVVRPGQQVMIREGQPVLRAIADSAGMMSWIG
                     QELFFHFRNTDFNAAISQVASWYGYTLSNPDNVKGTPLTCDLRRDPSPAGLVRQIGYM
                     ESGYAYLRLQGHVIQISKHPF"
     gene            complement(2271..2843)
                     /locus_tag="VKQ52_11215"
     CDS             complement(2271..2843)
                     /locus_tag="VKQ52_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ87806.1"
                     /translation="MSRKRDLASQFIAFRDGSPSAFAYFFRHYNVRIYYYLLRKVKNV
                     PAAQELTENAFLALFANHSKIESANHLLAFLYYFARHCSARFLQGQPCTEENGDWRVP
                     PASEILGILEDVEVIQNESQTMIQAEIQRLSRQRKKVVEMKFYLQLNVNTIAQNLGIS
                     PQTVRNHLSQSMLRLKNRLGEGFNNGLLFI"
     gene            3137..3688
                     /locus_tag="VKQ52_11220"
     CDS             3137..3688
                     /locus_tag="VKQ52_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87807.1"
                     /translation="MNRTTFAGALALIALFFTSCTNSGTAKSGDSATAATAVENPDMY
                     HGGGHSVPWTYIQPCIDAYIDTMNRYGINSDSPRQKITSCPDSTYMITTSEAFRADSL
                     MAYLKQQIDKYDPKGQGANLNFVIYPGIRTASMLTAVNVGTSTAGRITYFIVPDTSAN
                     PPKLGRITGGGDGSGFEVGGLQP"
     gene            3721..4377
                     /locus_tag="VKQ52_11225"
     CDS             3721..4377
                     /locus_tag="VKQ52_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87808.1"
                     /translation="MQLLYFQYFQLFSLLLAILCRKGLNAFSIGILIPILLVDNATEI
                     LGANYTRIFHSLNNYFVYNVYYLISTPLYLYLFATMLKGDARENRRLFVAGLAIELFI
                     IVNYFLFQGRAEFNTFTALLVSLTNIVLSCLVLTRLAIRKEDDSHLLLDPYFWINAVI
                     LLFSLVSIIVLGMQKYIALNHIQFGGKNLYFALMEAANAALYTGYSGAVLLCQTQRSK
                     "
     gene            4353..5138
                     /locus_tag="VKQ52_11230"
     CDS             4353..5138
                     /locus_tag="VKQ52_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLZ87809.1"
                     /translation="MPDAKEQITTTIFAVILVLLFFGVLFIVMLSYINYRRRQTAQEK
                     KAMQDQFDQQLMQTRLEIQEQTFRYLSEEIHDHVGQLLSLAKIQLSSFKEEGPIDFPM
                     LKDAGDNVARALQDLRSLAKGLHAGHIRHFSIREAVDEEVQRVRKAGGLTVSVTSEGA
                     EKKIKDDKQLILFRIIQECLQNCLKHAAATNINIGFTWYAEQLRVRVRDDGQGFDAER
                     ALAAANGLGLENIRNRARLTGGSSAIQSRPGEGTTVTVQIPYE"
     gene            5245..>6484
                     /locus_tag="VKQ52_11235"
     CDS             5245..>6484
                     /locus_tag="VKQ52_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HLZ87810.1"
                     /translation="MITELNAIVELEKQLNIIIPHKTGELSPAKIGYRCIDGRRVIGL
                     SLPNSHISDLSFFQNFEHLRELYLSNNNITDVDHLDGAVSLVTLWLNKNNITDIGPLA
                     KLTNLRELWLNENQIESISPLRHTDSLEQLWLSNNRIRDIVPLQDLYNLRILDLRSNP
                     IQKLPPWVVARGKMDVNWKNDRFYPEGITLYNNPLTDPPPEVVQLGKETMKAWFRSGN
                     AIPINELKILLVGHGGVGKTTLVKCLTGRSPDPDEKETHQINITNDRYTFNGQDISIN
                     FWDFGGQEVMHSTHQFFLSRRSLYLLVLDGRRDEDAEYWLKHIESFGGDSPVLIVLNK
                     TDTNPRYDVDRIFLQKKYPFIAGFCKTTCKPDNITGINSLYAAIESALDKVPLVRSKW
                     PKNWNAVKQDLTQIHEDYVTQ"
BASE COUNT         1541 a         1957 c         1590 g         1396 t
ORIGIN      
        1 cctcgatctt ccggatatcg ccggcaggga tgccgttcag ggcgctcgcc ccgttggggc
       61 cctgggcgga accagaaccg atgggcggaa gaaaaacacc ggaggcgaca ggaaccccgt
      121 ccaccacgac caggggttca tttccctgct ggatggaatg ctttccaccc agcaacacct
      181 cataggcgct ccctggaacc ccgttatact gtcggatgga aacgcctggc aaccggcctt
      241 ccaggttcga aagcaccccg ctgacaccgt cgatgcgttc gaacatctcc gcgctcctcc
      301 aggcatagga cgctgtggtg ctctcctgcg gccattgagg gctatatgcc gaaacctcca
      361 ccccgtcaag ctcctgcacc acattcgcca tcacaacaat attcggctgc ctgttggaaa
      421 gcgcaagctc cctatccgca tagccgacat ggctcactgt cacctccgaa gtaaaggccc
      481 ttacgggcag ccggaagcgc cccgccgaat cggtgagcac ggtcctgttg gagcctgtcc
      541 ggctcaccgt ggcgcctggc agcggttggc tttcgcaatc cagcaccacc ccttcgaggt
      601 cggcgtagat ggcgctcccc tcaccggcac cttctttata gccaaaaaag accgtcttat
      661 cttcgatcac atatcggatg ttcaacccgg caagacatgc cttcaaggcc ttatccagcg
      721 atgaatccag cggcagatgc accgctaccc ggggaatttg ttccatcagt cgcgaaggat
      781 agccgaccgt atagcccttc gactcgattt gccggaggac ctcggcgaaa gaatacttcc
      841 tgtctccggg atacctgtcg tctcccgggt aaccgtcatc accgtgaagg ggcacttggg
      901 cggggcacag gtaaatgcag gaaagcaggc cccccgacag gaaggttcgc cgaaccgtcg
      961 ctgcaagaat gctctctcga aatgctgacg ccctcatagt ggcttaatgt ggagttggtc
     1021 ctctcaaaat gggtgtttgg aaatttggat cacatgtccc tgcaatcgta aataggcata
     1081 tccgctctcc atatatccta tttgtcgtac cagtccggct ggtgaagggt cacgtcgcag
     1141 gtcgcaggta agaggcgttc cctttacatt gtccgggttg gatagcgtat acccatacca
     1201 ggaagccacc tggctgatgg ctgcattgaa atcggtattg cggaagtgaa agaacaattc
     1261 ctggccaatc cagctcatca tgccggcaga atcggcaatc gcccgcagca ccggctgccc
     1321 ctcccggatc atcacctgct ggccgggacg cacaacggtg tcatagtcca ccgccatcaa
     1381 ctttaccagg ccgctgaaca gcgatacccg ggtctcctgg cttcccttaa aggcctcgac
     1441 attgaacacc gtacccagca cctggatgtc ctcgccccgc ggagtttgaa ggtgaaaggg
     1501 cttgccggga tcatgcacga cgtcgaagaa ggcctccccc tctaagaaga atggggccgt
     1561 tgcctcccgt ccttccgacg gaaaggagaa ccgggagccg gcggccagcc atacattcgt
     1621 tccatccggc agtcgcaggt gaaactggcg ccgccgcctt ccgatcacca ggcgaagcaa
     1681 ggtccttccg ccggcagcgc cgggcgactg ggcgcttatt cgttgttttt cgccggccgg
     1741 tcgcgccctt attccctgtt tttcgtatgc catgccgttg gaatcgatct tccggagacg
     1801 atagtcgccc tcccggtcga aggtggtttc aaccgaaagg gtatccacac gggtggccct
     1861 gtcatcgctg ccgatgaaca cgacccctgc atcgtccggg atgtccctgc ctgtatgcgg
     1921 ggcatcagcg gcaggcggat gcattctttc ccacccacgc caaaagagcc aagcgccaca
     1981 ggcgaacaaa ataagaaaga cagcagccac cccgctcctc aaccatcggc gcccccatct
     2041 ctccggaaca tacgcgttca aggatacctg gtcttcgctg atcccccggg gatcagccag
     2101 gacttcggcc atccattgtt cgtcggtcga cagatgcgtc agtggccggt caaatcccga
     2161 tgtttccaac catttgacga gttggtccat ttcctcctcc gtcaatgctt caccgcgttg
     2221 acgctttacc agcagttgga taatatggtg gttgttggat gacatccgga ttatataaaa
     2281 aggagaccgt tgttgaaccc ttccccgagc cggttcttca aacgcagcat gctttgggaa
     2341 agatggttgc gtaccgtctg agggctaata cccaggtttt gtgcgatggt gtttacgttc
     2401 aactggagat agaatttcat ctccacgact tttttccgtt gacggctcag ccgctggatc
     2461 tcggcctgga tcatcgtttg gctctcgttc tggatgactt ccacgtcttc gaggataccc
     2521 aggatctccg aagcaggcgg aacgcgccag tccccattct cctccgtgca gggctgtccc
     2581 tgcaggaacc gggcgctgca atggcgggca aagtaataaa gaaaggcaag caggtgattg
     2641 gcgctctcga tcttgctgtg gttggcaaaa agcgccagaa aggcattctc cgtcaactcc
     2701 tgtgcggcag gtacattctt tactttacga aggagatagt aatagatgcg gacattgtaa
     2761 tgacggaaga agtaagcaaa ggccgatggg ctgccgtcgc ggaaggcaat aaattggctg
     2821 gcaaggtctc gttttcggga catgagcaat tagttttagg tttaaatgga cacagtaaca
     2881 taatcggtac gcgatgcaga aagtgaatct ttcagttcaa tggcatgttt ttatttttac
     2941 aaaaaaacga acagtcgtta gttaacccgc ctgccccggc gtaacgggcg ccgcgagctc
     3001 accatatata cgcataaggt atcccgtaaa aaaaactcgt cctttcttac aataataaac
     3061 cgtgtttttc tcctggcagg ttcggccgct gtccattatc ttgcagccca ttactaaaca
     3121 cctaaactca tctccaatga accgaaccac tttcgcgggc gcccttgcgc tcatcgcact
     3181 ctttttcacg tcatgtacca atagcggcac ggctaaatcc ggcgactcag ccacggcagc
     3241 aaccgctgtc gaaaacccgg acatgtacca tggcggcggc cattccgttc cctggacata
     3301 tatccaaccc tgtatcgacg cctatatcga taccatgaac agatatggca tcaacagcga
     3361 cagcccgcgg cagaagatca ccagttgtcc cgactccacc tatatgatca cgaccagcga
     3421 agccttccgg gcggacagcc tgatggccta cctgaaacaa cagatcgata aatatgatcc
     3481 gaaaggacaa ggcgcaaacc ttaacttcgt catctatccc ggcatccgaa ccgcatcgat
     3541 gttgaccgcg gtcaacgtgg gaacgtcgac ggccggccgg atcacctact ttatcgttcc
     3601 ggatacctcg gccaacccgc caaaactggg ccggataaca ggcggtggtg acggctccgg
     3661 tttcgaagta ggcggcctgc aaccctaaat cacctggcgc catccggcgc cgcgctatct
     3721 atgcagttac tctattttca atactttcaa ctgttcagcc ttttgctggc catcctgtgc
     3781 cgcaaagggc tgaacgcttt ttccatcggt atcctgatcc ctatcttact ggtggacaat
     3841 gcaaccgaaa tcctcggcgc caactatacg cgcatctttc actcgcttaa caactatttc
     3901 gtatataacg tttactatct tatttccacc ccgctgtatc tctacctctt cgccactatg
     3961 ctaaaaggcg acgcccgcga gaaccgccgc ctcttcgtcg ccggcctggc gatcgagctc
     4021 tttatcatcg tcaactactt cctcttccag ggcagggccg agttcaacac tttcaccgcc
     4081 ttgctcgtaa gtttgacaaa tatcgtcctc tcctgtctcg tgctcacccg cctcgccatc
     4141 cgtaaagagg atgactccca cctgctcctc gatccttact tctggatcaa cgcggtgatc
     4201 ctgttattta gtctcgtttc catcatcgtc ctcggcatgc agaaatatat tgccctcaac
     4261 catatccaat tcggcgggaa aaatctgtat tttgcactca tggaggcggc taacgccgcc
     4321 ctatacaccg gctatagcgg cgccgttcta ctatgccaga cgcaaaggag caaataacca
     4381 caaccatctt cgcagttatc ctcgtactgc tcttctttgg cgtattgttc atcgtgatgc
     4441 tctcctatat caactaccgg cggcggcaga cagcccagga gaaaaaggcc atgcaggacc
     4501 agttcgatca gcaactcatg caaacccgcc tcgaaattca ggaacaaacc ttccgctacc
     4561 tcagcgaaga gatccatgac catgtcggcc agctgctaag tcttgctaaa attcagctaa
     4621 gcagtttcaa ggaagaggga ccgatcgact tccccatgct aaaagatgcc ggcgacaacg
     4681 tcgcgcgcgc actgcaggac ctccgcagcc tggccaaggg actccatgcc ggccatatcc
     4741 gccacttcag catccgcgaa gccgtcgacg aagaagtcca gcgcgtccgc aaggccggcg
     4801 gcctcaccgt ctccgtcacc tccgaaggag cagagaaaaa aataaaggat gacaaacagc
     4861 tcatcctctt ccgtattatc caggaatgtc tacagaactg cctcaagcac gccgccgcca
     4921 ccaatatcaa catcggcttc acctggtacg cggaacaact ccgcgtccgc gtccgcgatg
     4981 atggccaggg cttcgacgcg gagcgcgcgc tggcggcagc caacggtctg ggcctcgaga
     5041 acatccgcaa ccgcgcgcgc ctcaccggcg gctccagcgc catccaaagc cggccggggg
     5101 aagggacgac cgtgacggtg cagatacctt acgaataagc cccgaccacc ccggggaaaa
     5161 cacccttttt gttcatccca gcccctccgt gcttccttat ccaagataaa ttcagtatca
     5221 tcatatccta atatcccgac aggaatgatt accgagctga acgcaatagt agagttggaa
     5281 aaacaactga atatcatcat cccgcataaa acaggagagc tgtcccctgc caagataggc
     5341 taccgctgca tcgatggccg aagagtcata gggttgagct tacccaattc gcatatcagc
     5401 gatctctcct tcttccagaa ttttgaacat ctcagagagc tatatctgag caataataat
     5461 attaccgatg tcgaccacct ggacggcgct gtctccttag taacactttg gttgaacaag
     5521 aacaatatca cggatatcgg cccgctcgcc aaacttacca acctgcggga gctatggctg
     5581 aacgaaaacc agatcgaatc catttctcca ctgcgacaca cggactcact ggaacaatta
     5641 tggctgagca ataataggat cagggatatc gttccgctcc aggacctgta taacctgcgg
     5701 atcctcgatc tgcgaagcaa tcccatccaa aaactccccc cctgggtcgt agccagggga
     5761 aaaatggatg ttaactggaa gaacgaccgg ttctatcccg aggggataac cctttacaac
     5821 aatccgctta ccgatccgcc acccgaggtt gttcaattgg gtaaagagac gatgaaggcc
     5881 tggttccgta gcgggaacgc catcccgatc aatgagctga agattctgct ggtgggtcat
     5941 ggcggcgtcg gcaagaccac gctggtgaag tgcctcaccg ggagatcccc cgatccggat
     6001 gagaaagaga cccatcagat aaatatcacc aacgatcggt acaccttcaa cggccaggac
     6061 atctccatca atttctggga ttttggcggc caggaggtga tgcatagtac ccaccagttc
     6121 ttcctcagcc ggcgcagcct ctacctgctg gttctcgatg gccgccggga cgaagatgcc
     6181 gaatactggt taaaacatat tgagagcttt ggcggagatt cgccggtgct gattgttctc
     6241 aacaaaacgg ataccaaccc gagatatgat gtcgacagga tcttcctcca aaagaaatac
     6301 cccttcatcg ccggcttttg caaaacaacc tgcaagcctg acaacatcac tggaatcaat
     6361 tccctgtatg ccgccattga atcggccctg gacaaagtgc cgctggtccg aagcaaatgg
     6421 ccaaagaact ggaacgcagt aaagcaagac ctcacgcaga tacacgagga ttatgttaca
     6481 cagt
//
LOCUS       DATGFG010000604         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_95854, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000604 DATGFG010000000
VERSION     DATGFG010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95854"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1041
                     /locus_tag="VKQ52_11240"
     CDS             187..1041
                     /locus_tag="VKQ52_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLZ87811.1"
                     /translation="MEKILLAMDAANLNMKAIDFGCYIARLTRSRLTGVFLEGLVSGL
                     NRKSGGDGTDPFGAGAWDSDADNPEVVPATVPSALAEANIRRFREACVCRDTLSLIHR
                     DRGIPVSEILAETRFADLLVIDPETSFSRADHGVPGRFVKDVLEASECPVLIAPYSFD
                     AIEEIVFTYNGTASAVYAIKQFTALFPELGNKKVFVVSVRQDETPAIEDQYKMKEWLK
                     AHYDNLEFVILKGDTSDELFGYLLEKKGSIVVMGAYGRSMLSRFFKPSQARLIVKTIN
                     LPIFIAHQ"
     gene            complement(1064..2056)
                     /locus_tag="VKQ52_11245"
     CDS             complement(1064..2056)
                     /locus_tag="VKQ52_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLZ87812.1"
                     /translation="MRTYSRQLLLGLFITVGVGLFALAILTVGSRQKAFSHTARIRIL
                     FDDAEGLQSGDNVRLSGVKIGTVKKVSLDEDNRVEVWIAVGEGDLAHIYTDTRGKIGN
                     DGFIGNRIVVLRGGTKAAGRVREMGSLMGERTVQTEDMLATLQENNKNLLVITGEMKV
                     IAHKINAGDGTVGLLLNDGRMADQLRRTVAGLNAAAVRTVEMTARLDEFAGELNDTGG
                     LAHRVVADTVVFRRLQEMMTELRTAVAAVSGAAAGIREASGGLNRENTPAGMLLHDQG
                     VAEDLRVTAGHLRTSSQKLDEDLEALQHNFLLRGFFRKKEKARRDSVAAAARRP"
     gene            complement(2083..2832)
                     /locus_tag="VKQ52_11250"
     CDS             complement(2083..2832)
                     /locus_tag="VKQ52_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87813.1"
                     /translation="MRTRFFIQVYGVSAFIIRFFRVAVTPPYEWREVIRQCREIGLRS
                     MGIITLTGFITGIVFTKQSRPSLADFGATSWLPSLVSLALVRALAPLITSLIIAGKIG
                     SNMGAELGSMRVTEQIDAMEVSSANPFKFLVVTRIVAICCMLPLLVTYMAFVGLAGSY
                     LNVHQNEQTSLRAFIDDAFGRISFLDLFASAFKALAYGFTIGVTGCYMGYTAGQGTVG
                     VGRAANSAVVVSMFLIFVEEILIVQVVNAIR"
     gene            2950..>3349
                     /locus_tag="VKQ52_11255"
     CDS             2950..>3349
                     /locus_tag="VKQ52_11255"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87814.1"
                     /translation="MNKWYPVALIMMIGSWLPQLPDLSLAGGHRALSSRPHPGVSTPR
                     HTGLSRHPDLVDRFYLRRNNRLFWFGPATTGAAGRQQLIASIDSAAWQGLDSNAYHPA
                     LLRQLANAGPRADSLLLRADRQYTGAALSLA"
BASE COUNT          711 a         1217 c          768 g          653 t
ORIGIN      
        1 caggcggcaa atctggcatt tgttatcatc cttaaattta cgaattcccc cgctgacccc
       61 attcccccgc cggccacaat tctcccgctt accgcaattt ccccgctgac tacaatcatc
      121 ttccccgatg acccccctca tcggaatccc tccccaccaa cccgaccttt ataaaaaaag
      181 gtacatatgg aaaagatact gctggccatg gacgccgcca acctcaacat gaaagccatc
      241 gacttcggct gttatatcgc ccggctgacc cgctcgcgtc tcactggcgt cttcctggaa
      301 ggcctggtca gcggtctgaa ccgcaaaagc ggcggcgacg gtaccgaccc gtttggcgcc
      361 ggcgcctggg actcggatgc cgacaatccc gaagtggtcc cggccaccgt tccctcggcc
      421 ctcgccgagg ccaatatccg ccggttcagg gaggcctgcg tctgccgcga taccctctcg
      481 ctgatccatc gcgaccgcgg tatccccgtc tccgagatcc tcgccgagac ccgcttcgcc
      541 gacctcctcg tcatcgaccc cgaaacctcc ttctccaggg ccgatcacgg cgtccccggc
      601 cgtttcgtca aggacgtcct cgaggcatcc gaatgccccg ttcttatcgc cccctacagt
      661 ttcgacgcga tcgaagagat cgtcttcacc tataacggca ccgcctccgc cgtatacgca
      721 ataaaacaat tcaccgctct ctttcccgaa ctgggcaata aaaaggtctt tgtcgtcagc
      781 gtccgccagg acgaaacccc ggcgatcgaa gaccaataca aaatgaaaga gtggctcaag
      841 gcgcattacg acaacctcga gttcgttatc ctaaaaggcg atacctccga tgaactcttc
      901 ggttatctcc tcgaaaaaaa gggctccatc gtcgtcatgg gagcctatgg acgatcgatg
      961 ctgtcccgct tcttcaaacc cagccaggcc cgcctgatcg tcaaaaccat caacctcccg
     1021 atctttatcg cccatcaata accttcatcc cctcatgtcg tcgtcacggc cgtcgcgcgg
     1081 ccgcggccac gctgtcccgt ctcgcctttt ccttctttcg gaagaaaccc cgtaacagga
     1141 aattatgctg caaagcctcc aggtcctcgt ccagtttttg actgctggtc cgcagatgac
     1201 cggccgtaac ccgcaggtct tcggccacgc cctggtcatg cagcagcatc cccgccggcg
     1261 tattctccct gttcaatccc ccgctggcct cccggatacc cgccgcggcg cccgaaacag
     1321 ccgctaccgc agttcgtaac tccgtcatca tttcctgcag ccggcggaag accaccgtgt
     1381 ccgccaccac ccggtgcgcc agcccgcccg tatcgttcaa ttcccccgca aattcatcca
     1441 gcctcgcggt catttccacc gtcctgacag ctgcggcatt caatccggcc accgtccgcc
     1501 gcaattgatc cgccatccgc ccatcgttca gcagcaggcc caccgtgccg tcgccggcat
     1561 tgatcttgtg ggcgatcacc ttcatttcgc ccgtgatcac cagcaggttc ttgttgttct
     1621 cctgcagcgt cgccagcatg tcctccgtct gcaccgttcg ctcacccatc aggctaccca
     1681 tctcgcgtac cctccccgcc gccttcgtac ccccacgcaa taccacgatc cggttgccga
     1741 tgaacccatc gttgccgatc tttcctctcg tatcggtata gatatgcgcc aggtccccct
     1801 caccgacagc gatccacacc tcgacccggt tgtcttcatc caggctcacc ttcttcaccg
     1861 tccctatctt gacgcccgat aaccggacat tatcccccga ctgcaatcct tcggcatcat
     1921 caaagaggat gcgtatcctc gcggtatggg aaaaagcctt ttgccggctg cccaccgtca
     1981 ggatggcgag ggcgaacaac ccgaccccca cggtaataaa aagacccagt aataactggc
     2041 ggctatacgt tcgcatacat tggatatttt aacccgatcc attcatctta tggcattcac
     2101 cacttgtacg atcaggatct cttctacaaa aatgaggaac atcgatacca ccaccgcgga
     2161 gttcgcagcc ctgcccacgc ccaccgtacc ctgcccggcc gtgtacccca tataacaacc
     2221 cgtaacaccg atggtaaacc catacgccaa cgccttgaat gccgaggcga acaggtccag
     2281 gaaggagatg cggccaaagg catcatcgat aaaagcccgc aggctcgtct gctcattctg
     2341 atgcacgttg agataagaac ccgccagccc gacaaacgcc atataggtga ccagcaacgg
     2401 caacatgcaa cagatggcca ctatccgcgt gacgaccagg aatttgaaag gattggccga
     2461 ggaaacctcc atggcgtcaa tctgctcggt tacccgcatc gaccccaatt cggcccccat
     2521 attcgacccg atcttgccgg cgatgatcag cgacgtgatc agcggcgcca atgcccggac
     2581 aagcgccagc gaaacaagcg agggaagcca ggacgtagcc ccgaaatccg caagtgatgg
     2641 ccgcgactgc ttggtaaaga cgatccctgt tataaaaccc gtcagcgtga tgatgcccat
     2701 gctccgcagc ccgatctccc ggcactgccg gatcacctcc ctccactcat acggcggtgt
     2761 cacggccacc cggaagaacc ggatgataaa ggccgatacc ccgtacacct ggataaaaaa
     2821 cctggtcctc atcgaataaa gatagcaggg gagcacagcg ggaaacatga ccggactcac
     2881 cccgcccact gaccatgctc agcaggcaac cctggtgacg gcgctacctt gctttcttca
     2941 aacctccgta tgaacaaatg gtatccggtc gcattgatca tgatgatagg ttcctggctg
     3001 ccgcagctac ccgatctcag tctggccggc ggccacaggg cgctttcatc ccgtccccat
     3061 ccgggcgttt ccaccccccg gcacaccggt ctctcccggc acccggatct cgtggatcgc
     3121 ttctaccttc gacgaaacaa ccgcctcttc tggttcggac cagccaccac cggcgcagcc
     3181 ggccggcaac aactgatcgc ctccatcgac agcgccgcct ggcagggcct cgatagcaat
     3241 gcctaccatc ccgccctcct ccggcaactg gccaacgccg gaccccgcgc cgacagcctc
     3301 ctgctgaggg ccgatcgcca atacaccggg gccgcgctct cccttgccc
//
LOCUS       DATGFG010000605         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_141792, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000605 DATGFG010000000
VERSION     DATGFG010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141792"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VKQ52_11260"
     CDS             complement(<1..1302)
                     /locus_tag="VKQ52_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HLZ87815.1"
                     /translation="MVAIGQDALSIDDFSDILFNKGTVTLGKEELEKVDTNFRFLREF
                     SSDKLIYGLNTGFGPMAQYKVSQENLIRLQYNLIRSHSSGSGRLLPVELIKATMIARL
                     NSLMRAYSGVHTDVVELLRDLINKDIYPCIYEHGGVGASGDLVQLAHLGLAMIGEGEV
                     VYEGAVYPAMEVFGRLRLKPLSITGREGLAILNGTSAMTGIGMVNIIRAWQLLEWSVL
                     LSSMVNEVVEAYDDHYSHELNIVKRHRGQNAIAAMMRDILKESGMVRRRAEHLYDPVN
                     IEQEVFEDKIQEYYSLRCVTQILGPIYDTILQAERTVTDELNSVNDNPVIDHLNHNVF
                     HGGNFHGDYVSLEMDKLKIAVTKLSMLSERQLNYLLNDKLNQKFPPFLNLGVLGFNFG
                     MQGMQFTATSTVAENQTLSFPMYVHSIPNNNDNQDIVSMGTN"
     gene            complement(1311..2624)
                     /locus_tag="VKQ52_11265"
     CDS             complement(1311..2624)
                     /locus_tag="VKQ52_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLZ87816.1"
                     /translation="MSFHDITYRSAGELKLLQEEKLQTLLPWLQEHSPFYRRLFSLHN
                     LQPETIRRLEDLPGIPTTTKEDLQQFNWDFLCVEKNKIIEYTSTSGTLGSPVTIALTE
                     NDLDRLAYNEQASFSTAGGSAADRYQLMLTLDRQFMAGMAYYAGARKMGAGVIRLGPG
                     APSLQWETIFRLQPTTIVAVPSFIIKLIQYAQDHGIDLRRCSVEKAICIGEGIRNPDF
                     SWNVLGEKIREAWNIRLYSTYASTEMQTAFTECEEGRGGHLRPELLIVELLGDDDRPV
                     PPGSPGEVTITTLAVEGMPLLRYKTGDICTYDTNPCACGRNSLRLSPVLGRKKQMIKF
                     KGTTLYPPALFDLLNGMAEIRDYVVEVSTNELGLDEVLLYLQPAALTDDTDHRIRSWL
                     QARLRVIPSIRYVRPEDIQRLQVTESNRKLIKFIDKRGDALPVQK"
     gene            2791..>3419
                     /locus_tag="VKQ52_11270"
     CDS             2791..>3419
                     /locus_tag="VKQ52_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87817.1"
                     /translation="MKKILLVLLLPAGIVSCHHKEKILVGRPLIDSLISHSVTPVIAT
                     QNQAELLFWLRRIDTVVPGYLNESRYAGCLVQSFRLFGVIDSLRKADSVLHAIDRNFN
                     HKETSADMALVSLCITGHRFREADSLLQKAKQLGLRPYESHAASFDVAFELGNYFQAR
                     TELNAIQSPGDYGYYFRKSKIDHLDGELDSAISDMNKAALLAETNDYLKG"
BASE COUNT          798 a          896 c          876 g          849 t
ORIGIN      
        1 gttagtgccc atgctgacga tatcctggtt gtcattatta ttggggatgc tatgcacata
       61 catcgggaaa gacagggtct ggttctccgc aacggtggag gtggccgtga attgcatgcc
      121 ctgcatgccg aagttaaagc ccagcactcc cagattgaga aaagggggaa atttctggtt
      181 cagtttgtcg ttcaataaat aattcaactg ccgttcggat aacatggaca atttggtcac
      241 ggcgatcttt agcttgtcca tttccagcga gacataatcc ccgtggaaat tcccgccatg
      301 aaaaacattg tgattgagat ggtcgatcac gggattgtcg ttcacagaat tcagttcatc
      361 cgtcactgtt cgctcagcct gcaggatcgt atcatagatg gggccaagga tctgcgtgac
      421 acagcgcagg gaatagtatt cctggatctt atcctcgaat acttcctgtt caatgttgac
      481 gggatcgtat aaatgctccg cccgtctgcg gaccattccg ctctccttca ggatatcccg
      541 catcatagcc gcgatggcgt tctgcccccg gtggcgcttt acgatattca attcgtggga
      601 atagtgatcg tcgtaggctt ccaccacttc atttaccatg gacgacagaa gtaccgacca
      661 ttccaacaat tgccaggccc ggatgatatt gaccatgccg atgcctgtca tggccgatgt
      721 cccgttcagg atagccaatc cttccctccc ggtgatggaa agcggtttta gccgcaggcg
      781 gccgaagacc tccatagccg gataaacggc gccctcatag acaacctctc cttcgccgat
      841 catggcaagg cccaggtggg ccaattgaac aaggtctccg ctggcgccca cgccgccatg
      901 ttcgtagatg cagggataaa tatctttatt gatgaggtct cgcagtaatt ccacgacatc
      961 cgtatgaacg cccgaatagg ccctcatcag gctgttgagg cgggcgatca tcgtagcttt
     1021 tatcagttcc acgggcagga ggcgaccact gcccgaacta tggctacgga tcaggttgta
     1081 ttgcaggcgg atcaggtttt cctggcttac cttgtattgg gccatggggc cgaagccggt
     1141 attcaacccg tagatcagtt tatcggaaga gaattccctg agaaatctga aattggtatc
     1201 cactttttcc aattcctctt tacctaaggt aacggtacct ttgttaaata aaatgtctga
     1261 aaaatcgtca attgaaaggg cgtcctggcc aatcgcaacc atgggcaaaa ttatttttga
     1321 acgggtaggg catcgcccct tttatcaatg aatttaatta atttcctatt ggattccgtc
     1381 acctgcaatc gttgaatgtc ctcgggcctt acgtatctga tcgatggtat gacccttaac
     1441 ctggcttgca gccagctcct gatcctatga tccgtatcgt cagtcagggc ggcaggctgc
     1501 aaatatagta agacttcgtc cagccccaac tcattggtgg aaacttccac cacataatcc
     1561 cggatctcgg ccatgccatt gagcagatcg aacagcgccg gagggtagag ggtagtccct
     1621 ttgaatttga tcatttgttt tttgcgtccc agcaccgggg acaatcgcag gctattcctg
     1681 ccacaggcac agggattggt gtcataggtg cagatatcgc cggttttata tcgcaaaagc
     1741 ggcatgcctt caaccgccaa tgtcgtaatc gtcacttccc cgggagaacc tggcgggaca
     1801 gggcggtcat cgtcacccaa tagctcgacg atcaatagtt ccggccgcaa atgtccgccg
     1861 cgtccttctt cgcattcggt aaacgccgtc tgcatctccg ttgaagcata ggtcgaatac
     1921 aaacggatat tccaggcttc cctgatcttt tcgcccaaca cattccacga gaaatcgggg
     1981 ttgcggatgc cttccccgat gcagatggcc ttttcaaccg agcagcgtct caggtcgatg
     2041 ccatggtcct gtgcatattg gataagcttg atgataaaag aggggacggc cacgatggtc
     2101 gtaggctgga gacggaagat ggtctcccat tgcagggatg gcgcccccgg gccaaggcgg
     2161 atcacgccgg cccccatttt tcgggccccc gcataataag ccatgcccgc catgaactgc
     2221 ctgtccaggg taagcataag ttgatatctg tccgcggccg accctccggc ggtggaaaag
     2281 gaggcctgtt cattataagc cagccggtca agatcgttct ccgtcagggc gatggttacc
     2341 gggctgccaa gggtccccga agtcgaagta tattctatta ttttgttttt ttctacgcac
     2401 aaaaaatccc agttgaattg ttggagatcc tcttttgtag tggtcgggat accaggcaga
     2461 tcttccagcc tcctgatcgt ctccggctga aggttatgta aggaaaacag cctgcggtag
     2521 aagggtgagt gctcttgcaa ccagggcagt aaggtttgca gcttttcttc ctgtaataat
     2581 ttcaactcgc cggccgaccg gtatgtgatg tcgtgaaagc tcatgatccg ctaaaataca
     2641 atagattcgc aggtatggca aagcaaatat tcatgatcgg ctgtttttca tatccgcagc
     2701 tgcgatccgg ccgggttcgc gccctggaag aacaggcagc acaggacatc tgaatgatta
     2761 gccttttcgt acatgaggga aaccctcgtc ttgaaaaaga tactcctggt cttgctgctg
     2821 cctgcaggga tcgtctcctg tcatcacaaa gaaaagatac tggtcggccg gccattgatc
     2881 gacagcctga tctcccactc ggtcactccg gttatcgcaa cgcaaaacca ggcggaattg
     2941 ctgttctggc tccgccgcat cgataccgtt gttcccggct atctcaatga atcacggtat
     3001 gccggttgtc ttgtccaatc cttccggctc tttggcgtga tcgacagcct gagaaaggct
     3061 gacagcgtcc tgcatgcgat agaccggaat ttcaaccaca aggaaacatc cgcggatatg
     3121 gcacttgtct cgctctgcat caccggtcat cgtttcaggg aagccgacag cctgctgcaa
     3181 aaagcgaagc aattggggct gcggccctat gagtcccatg ctgcgtcctt tgacgtagcc
     3241 ttcgaactgg ggaattattt ccaggcaagg acggagctga atgccattca gtcgcctggt
     3301 gattatggct attactttcg aaaatcgaag atcgatcacc tggatggaga actggacagc
     3361 gcgatcagcg acatgaacaa ggctgccttg ctggcagaga caaacgatta cctgaaggg
//
LOCUS       DATGFG010000606         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_248337, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000606 DATGFG010000000
VERSION     DATGFG010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248337"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VKQ52_11275"
     CDS             <1..364
                     /locus_tag="VKQ52_11275"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87818.1"
                     /translation="TAGKILFVEDTGEYLYSIDRMFWHLQRTGKLDRLAGLVVGGFKV
                     KPDDPGEEFGRSVYDIVTDRVKSYGYPVCFDFPVGHQKENYALKYGIPHLLDVRDGGV
                     TLTDGSVPAPPSSAPQSK"
     gene            366..1274
                     /locus_tag="VKQ52_11280"
     CDS             366..1274
                     /locus_tag="VKQ52_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HLZ87819.1"
                     /translation="MPIIPPFLRPGDTIAIVCPAGYMDPEKAQTCIGTLESWGYHVRT
                     GKTLGGASPTYFSGTDDQRLADFQQMLDDPEVKAILCGRGGYGLSRIIDRIDFGRFAK
                     TPKWIIGFSDITVLHTHILTRLGIATLHAPMAGAFNDGGAAGPGVVSLRHALLGESAV
                     HRAPAHPFNRPGAASGPLIGGNLAILAHLTGSVSEPDTDGCLLFLEDVGEYLYNIDRM
                     LHQLLRSGKLRNLAGLLIGGFTEMKDTIRPFGATVEEIIRDIAADAAYPVAFDFPVSH
                     GKENVALKVGATYRLEVTPAGTILQE"
     gene            complement(1280..2110)
                     /locus_tag="VKQ52_11285"
     CDS             complement(1280..2110)
                     /locus_tag="VKQ52_11285"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HLZ87820.1"
                     /translation="MQTALKYRSLLSVFVLTLAVAGYPGEGMAQRFNHGGFSRPAPAA
                     PARVAVPPMRQEARPAAPPARPEIRPDERPTERPTINGGGRNIGNHDFSRPVDVHQTV
                     NVHETVNVRDHVNVYHTGGYRGIHPYYYHPYRPYYWGPHWHPLGFFLTALATDAIFFS
                     FNSQRYYYDDGVYYQPANGGYSVVPAPVGALVAALPPGYETTMVGNDTYYYFGGAFYV
                     YTGQGYQVVVAPAGAVITQLPVGAVEQQINGDTLLVYNNTYYLPISQDGQDAYEVVNP
                     "
     gene            complement(2134..2874)
                     /locus_tag="VKQ52_11290"
     CDS             complement(2134..2874)
                     /locus_tag="VKQ52_11290"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HLZ87821.1"
                     /translation="MYKKALFFLSVCFLSAQLQAQTRRIDTVAVAILDRMTAVIGDLG
                     SCSFTVKSNYDVSSKELGLIKHSDEEQVYLHGPNKLLLKSDGDRGSRDFYFDGKKLSY
                     YSLDKNQYGQIDAGMSLMEMIDTVNKLYGIEFPAADFLYPTFVDDVLAESKELVYLGL
                     TKVDGKECYHIAGKGADKTFQFWISDDAFSLPLKMVIVYTGREMNPQYEALLTDWQVN
                     PNLPDAIFGFTAPHKAKKVRLVPLSHKK"
     gene            3001..3936
                     /locus_tag="VKQ52_11295"
     CDS             3001..3936
                     /locus_tag="VKQ52_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLZ87822.1"
                     /translation="MTFVQLEYIVAVDTYRHFATAAGHCFVTQPTLSMQVQKLEEELG
                     LKIFDRSKQPVIPTEAGGEIIEQARRILGEKHLISEIVQEKKGVLAGELRIGIIPTLA
                     PYLLPLFVQQFTAKYPQIKLVVNELTTEMVVARLREGRIDVGILVTPLQENGIREQVL
                     FYEELLVYVSRKNAAYKKTYMLAQDIDPNKLWLLEEGHCFRSQIVRLCELRKASKEGS
                     HFDYEAGSLETLRRMVELNDGITILPELAALDLTGKQQQLVRHFKRPAPMREVSLVVH
                     RDFVKQRLVQALHQEILRSVPEKVRQNKNQNVIPI"
BASE COUNT          928 a         1230 c         1132 g          892 t
ORIGIN      
        1 caccgcgggc aagatcctct ttgtcgaaga caccggcgag tatctctaca gtatcgaccg
       61 catgttctgg cacctgcagc gtaccggcaa actggaccgg ctcgccgggc tcgttgtcgg
      121 cggctttaag gtaaagcccg atgaccccgg cgaggagttt ggccgatcgg tgtacgatat
      181 cgtcaccgac agggtcaaat cctatggtta ccccgtctgc ttcgactttc ccgtcggtca
      241 tcaaaaagag aactatgcac ttaaatacgg gatcccgcat ctgctcgacg tacgggatgg
      301 aggggtgacc ctgacggatg gctctgttcc cgcaccccct tcttccgccc cacaatcaaa
      361 atagcatgcc catcattccc cccttcctcc ggcccggtga cactattgcg atcgtctgcc
      421 cggccggcta tatggacccc gaaaaagcgc aaacctgtat cgggacgctc gagtcctggg
      481 gctatcatgt ccgtaccggc aaaacactgg gcggcgcatc gccgacatat ttctccggca
      541 cggatgacca gcgtctcgcc gactttcaac agatgctgga tgatcccgaa gtaaaggcca
      601 tcctctgcgg gcgcggcggc tatggcctca gccggatcat cgaccggatc gactttggcc
      661 ggtttgcaaa aacacccaaa tggatcatcg gcttcagcga tatcaccgtg ttacatacgc
      721 atatcctcac ccgactggga atagcgacgc tgcatgcccc gatggcgggt gcattcaacg
      781 acggtggcgc cgccggcccc ggtgtggtgt ccctgcgtca cgccctcctg ggtgaaagcg
      841 cggtccaccg ggctcccgcc catcccttca accgccccgg cgcggcttcc ggcccgctga
      901 tcggcggcaa cctcgcgatc ctcgcccatc ttaccggcag tgtctcggag ccggacaccg
      961 acggctgtct cctcttcctc gaggatgtcg gggaatacct gtacaatatc gaccgcatgc
     1021 tgcatcagct gctgcgcagc ggcaagctcc ggaacctcgc cggtctgctg atcggcggct
     1081 ttacggaaat gaaagatacc atccgcccct ttggcgccac tgtggaagag atcatccgcg
     1141 acatcgccgc cgatgccgcc tatcccgtgg cctttgattt tcccgtcagc cacggaaaag
     1201 aaaatgtggc gctcaaagtg ggcgccacct atcgattgga agtcaccccc gcggggacta
     1261 ttctccagga atagaaagtc tatggattaa ccacctcata ggcgtcctgg ccatcctgcg
     1321 agatgggcag gtagtaggtg ttgttataca ccagcagggt atcgccgttg atctgctgct
     1381 ccacggctcc aacgggcagc tgggtgatga ccgcgccggc cggcgcaacc actacctggt
     1441 atccctgacc ggtgtagaca tagaacgctc ccccaaaata ataatacgtg tcattgccca
     1501 ccatcgtcgt ctcatatccc ggcggcaggg cggcgacaag cgctcccacc ggcgcgggca
     1561 cgacggaata accgccgttg gcgggctggt aataaacgcc atcgtcataa tagtagcgct
     1621 ggctgttaaa gctgaagaag atggcgtcgg tcgccagcgc cgtcaggaaa aagcccagtg
     1681 gatgccagtg cggtccccag tagtacgggc ggtacggatg gtaatagtaa ggatggatac
     1741 cgcgatagcc acccgtatga tagacattca catggtcgcg tacgttcacc gtctcatgga
     1801 cattcaccgt ctggtggacg tcgacgggtc ggctgaaatc atggttgccg atattccggc
     1861 cgccgccatt gatggtgggc cgttcggtgg gccgctcatc gggccggatc tccggccgcg
     1921 ccggaggcgc ggccggcctt gcctcctggc gcatgggcgg aactgcaacc cgtgcaggcg
     1981 ccgccggtgc gggccggcta aaaccaccat ggttgaatcg ctgggccatg ccctcccctg
     2041 gatagccggc taccgccagc gtcaatacga agacagagag gagagagcga tattttaaag
     2101 cggtttgcat aactgagtat tgaggataat aatttatttt ttatgggaca aaggaaccag
     2161 gcggaccttt ttggctttat gcggggcggt aaaaccgaag atcgcatccg gcagattggg
     2221 attcacctgc cagtcggtga gcagggcctc gtactgcgga ttcatctccc tgccggtgta
     2281 aacgatcacc attttcaacg ggaggctaaa ggcgtcgtcg gagatccaga attgaaaggt
     2341 cttgtccgcc cctttgccgg cgatgtgata acattccttg ccgtccacct ttgtcaaccc
     2401 gagatagacc agctccttcg actcggccag gacatcatcc acgaaggtgg gatagaggaa
     2461 atcggccgcg gggaactcga tgccgtacag tttgttcacg gtgtcgatca tctccatcag
     2521 ggacatgccg gcatcgatct gcccatactg gttcttgtcg agggagtaat agctcagctt
     2581 tttcccgtcg aaataaaaat cccggcttcc gcggtcgccg tccgacttta gtaacagctt
     2641 gttggggcca tgcaggtata cctgctcttc gtcggaatgt tttatcagcc ccagttcctt
     2701 gctgctgaca tcataattcg acttcaccgt aaaagaacag gaaccaaggt caccgatgac
     2761 cgcagtcatc cggtcgagga tggcgaccgc cacggtgtcg atccggcggg tctgcgcctg
     2821 aagctgcgca gacaaaaagc aaacggaaag aaagaatagc gcttttttat acatggtctt
     2881 ccattttgaa tttattcctg caaggtttgg aaaagcgggt aactcccggc aaaatggatc
     2941 gttatttatt cgttaacaca atagttatta cttttaccat ttcatagaat ttatctatat
     3001 atgacctttg tacaactgga atatatcgtc gccgtggaca cgtatcgtca tttcgccact
     3061 gccgcggggc attgcttcgt tacccagccc acccttagca tgcaggtgca gaaactggaa
     3121 gaggaactcg ggctcaagat cttcgatcgc agcaaacaac ccgttattcc caccgaggcg
     3181 ggtggtgaga tcatcgaaca ggcccgcagg atcctcggcg aaaaacacct catctccgag
     3241 atcgttcagg aaaagaaagg cgtccttgcc ggcgaactgc gcatcgggat cattcccacg
     3301 ctggctcctt acctgttgcc tctctttgtg cagcagttca cggcgaaata cccccagatc
     3361 aaactcgtcg tcaacgagct gaccaccgaa atggtcgtcg cccgcctccg cgaaggccgg
     3421 atcgatgtcg gtatcctggt caccccgctc caggagaacg gcatccggga acaggtgctc
     3481 ttctatgaag agctgctcgt ctatgtctcc cggaagaatg ccgcctataa aaagacctat
     3541 atgctcgccc aggacatcga tcccaacaaa ctatggctgc tggaggaagg acactgtttc
     3601 cgcagccaga tcgtccggct ctgcgaactg agaaaggcca gcaaggaagg cagtcacttc
     3661 gactatgagg ccggcagcct cgaaaccctt cgccggatgg tcgaactgaa cgacggcatc
     3721 accatcctcc ccgaactggc ggcactcgat ctcacgggta agcagcaaca gctcgtccgt
     3781 cacttcaagc ggccggcgcc gatgcgggag gtaagtcttg ttgtccaccg cgactttgtc
     3841 aaacaacgcc tcgtccaggc gcttcaccag gagatcctcc ggtctgtgcc cgagaaggtg
     3901 cggcagaaca agaaccagaa tgttatcccg atatgagggc gatcaaccgg tatccgatat
     3961 gagacccctt aaccgatata ccaccgatta aatttcgacg atttttttgc tgcaatcctt
     4021 aatttgcagc cgatatggct atcaaaaaga tattgcttaa cgtattaggc gagaaaaaat
     4081 acctgtcctt cctggccggg agcttccagc ggctgtatag cgccggtctc ctggggaaag
     4141 aatatgccga tgtctttttt ctgaagaagt ttatccggaa gg
//
LOCUS       DATGFG010000607         5200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_329949, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000607 DATGFG010000000
VERSION     DATGFG010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329949"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VKQ52_11300"
     CDS             complement(<1..898)
                     /locus_tag="VKQ52_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLZ87823.1"
                     /translation="MSNYPYQIRSLEQYRSEYKKSVEDPEGFWAGIAANFQWRRPWDK
                     VLEWNFTEPKVRWFGGAQLNITENCLDRHLAERGDEPAIIWEPNDPEEHFRILSYREL
                     HAKVCQFANVLKNNGMKKGDRACIYMPMVPELAIAVLACARIGVIHSVVFGGFSAQSI
                     ADRIQDAQCSLVITSDGANRGNKQIPLKSVIDDALVQCPSVKKVIVLTRTRVAVSMIK
                     GRDVWWEDEIRKVETQGNPPCPVEVMDAEDLLFILYTSGSTGKPKGVVHTCGGYMVFT
                     NYSFVNVFQYRPGDVFFCTADIG"
     gene            957..2504
                     /locus_tag="VKQ52_11305"
     CDS             957..2504
                     /locus_tag="VKQ52_11305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ87824.1"
                     /translation="MPSLSTTSTKDIRKIIIASSVGTLIEWYDFYIFGSLATVIAGQF
                     FPKSNPTAAILSTLATFAAGFIVRPFGALVFGRIGDLIGRKYTFLLTLIIMGGSTFAI
                     GLVPNYEHIGFAAPLIVLVLRLMQGLALGGEYGGAATYVAEHSPGHQRGFYTSWIQAT
                     ATIGLFLSLGVILLTRHLLNADQARSIEIFNDKGWRIPFLLSIFLVAISVYIRLKMQE
                     SPLFSQLKTEGKLSTNPLKESFGHKANLKIVLLALFGATMGQGVVFYTGQFYAQSFLE
                     NVCKIDFDQSKTMLLFGILLATPFFVVFGSWSDKVGRKWIMMIGMLLAILTYPFLFKQ
                     LLVHSNPEGRTEFAEQKEIRTSVAFINTTSRDVIRTSATLSHYDGGLLVTETKKDTVY
                     ADGHTGKKPVVTVSRTLGTADYWKITGVLFLMVFYVTMVYGPIAAFLVELFPTRIRYT
                     SMSLPYHIGNGVFGGMTPFIATLLTTIYTGDKLVGLWYPIGVTAVCLVIGVIYIPAKG
                     STETFDQ"
     gene            2527..2769
                     /locus_tag="VKQ52_11310"
     CDS             2527..2769
                     /locus_tag="VKQ52_11310"
                     /inference="COORDINATES: protein motif:HMM:NF044889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87825.1"
                     /translation="MNLLKKALGIVWIALGPLVLFYLIKTGASEITKKPVIDTRIQWG
                     VFIGIFIPIAVGIVLFGYYALKGEYGTESRTPKGNN"
     gene            2870..3781
                     /gene="gldA"
                     /locus_tag="VKQ52_11315"
     CDS             2870..3781
                     /gene="gldA"
                     /locus_tag="VKQ52_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HLZ87826.1"
                     /translation="MSIEIKDLVKIYGEQRAVDTISFQVGKGEIVGFLGPNGAGKSTT
                     MKILTGYLGQDGGEARVCGISVKESPLETKKKIGYLPEANPLYPDMYVREYLDFIADV
                     QQVTDKKARIEAVIALVGLTEESKKKLGQLSKGYKQRVGLAAALIHNPEVLILDEPTS
                     GLDPNQIIEIRDVIKRLGKDKTVLFSSHILQEVEALCDRVVIINRGKLVADSLISRLR
                     TEGSEANTIRVAFREQLEPAALGEINGIRSLQRLSANEWELQTEEPDEVKKQLLELAL
                     QNKWNIVSLQSENKSLEAIFRSLTNQD"
     gene            3805..5088
                     /gene="eno"
                     /locus_tag="VKQ52_11320"
     CDS             3805..5088
                     /gene="eno"
                     /locus_tag="VKQ52_11320"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLZ87827.1"
                     /translation="MSYIADIHARQILDSRGNPTVEVDVVTENNHRGRAAVPSGASTG
                     IHEAVELRDGDKKVYSGKGVLKAVANVNDIIADQLIGFPVNEQASLDARLIELDGTEN
                     KGKLGANALLAVSMAVAKAAAKESKLPLYRYLGGVNSTVLPMPLMNILNGGVHADNKI
                     DYQEYMIVPVGAETFSDGLQWGVEIFHTLKSVLKKKGYSTNVGDEGGFAPDIQSNEEA
                     IETVLQAIEAAGYKVGSQIGIALDAASSEMFKDGKYKFYKSSGKELTSDQMVEYWTSW
                     VNKYPIISIEDGMAEEDWDGWKKLTDAIGNKVQLVGDDLFVTNTKILQKGIDRGVANS
                     ILIKVNQIGTITETINAVQLAQTHGYTTIMSHRSGETEDTTIADLAVALNCGQIKTGS
                     ASRTDRLAKYNQLLRIEEELGDAGIYPKGRIKFKK"
BASE COUNT         1268 a         1537 c         1332 g         1063 t
ORIGIN      
        1 aaccgatgtc cgccgtacag aaaaagacat cccccggccg gtactggaac acattgacaa
       61 aactgtagtt ggtaaagacc atatagccgc cacaggtatg caccaccccc ttcggctttc
      121 ccgtcgaacc ggaagtatac aggatgaaaa gcaggtcttc cgcatccatc acttccaccg
      181 ggcagggcgg attaccctgg gtctccacct tgcggatctc gtcttcccac catacatccc
      241 ggcccttgat catgctgacg gcgacacgcg tacgggtaag aacgatcacc ttctttacgc
      301 tgggacattg tactaaggcg tcatcgatca ccgacttcag cgggatctgc ttattgccgc
      361 ggttggcgcc gtcggaagta atgacaagac tacattgtgc gtcctgtatc ctgtctgcaa
      421 tgctttgtgc ggaaaatccg ccaaaaacca ccgagtggat caccccgatc cgggcgcagg
      481 ccaggacggc gatcgccagc tcgggaacca ttggcatata gatacaagcc cggtcgcctt
      541 ttttcatccc gttgttcttc aacacattgg cgaactggca aaccttggcg tgcaattccc
      601 tgtaagacaa aatgcgaaaa tgctcctcgg gatcgttggg ttcccaaatg atggcgggtt
      661 catcccctct ctcggccagg tggcgatcaa ggcagttctc ggtgatattc agctgcgcgc
      721 cgccgaacca acgcaccttc ggctcggtaa aattccattc cagtactttg tcccagggac
      781 ggcgccactg aaagtttgcg gctatacctg cccagaatcc ttccggatct tccacacttt
      841 ttttatattc ggaccgatat tgttcgagtg accggatctg atagggataa ttgctcatga
      901 cacgaaatag atttggggcc ataaatgtag atcaaaatat tttatcttta gccgctatgc
      961 catcattatc cactacttcg acaaaagaca tccgcaagat catcatcgcc tcatccgttg
     1021 ggacactcat cgaatggtac gatttctaca tcttcgggag cctggcgacc gtcatcgccg
     1081 gtcagttctt tcccaaatcg aatcccaccg cggccatcct gtcgacgctc gcgacctttg
     1141 ccgccggttt catcgtgcgg cctttcggcg ccctggtctt tggacgcatc ggtgacctca
     1201 tcggccggaa atacaccttc ctgctgaccc tgatcatcat gggcggctcc acctttgcca
     1261 tcggcctcgt gccgaattat gagcatatcg gttttgcagc cccgctgatc gtcttagtcc
     1321 tgcgcctcat gcagggtctt gccctcggcg gggaatatgg cggcgccgcc acctatgtag
     1381 cagaacattc tccgggccac caacgcggtt tctacacctc ctggatccag gccaccgcca
     1441 ccatcggcct cttcctcagc ctgggcgtca tcctgcttac ccgccacctg ctcaacgcag
     1501 accaggcgcg gagcatcgaa atattcaacg acaagggctg gcggatcccc ttcctcctct
     1561 ccattttcct ggtggcgatc tccgtctata tccgcttgaa aatgcaggaa tcccccctgt
     1621 tcagccagct gaagacggaa ggcaaactat ccaccaatcc gctgaaagaa agcttcggcc
     1681 acaaagccaa cctgaagatc gtcctgctgg ccctgttcgg cgccacgatg ggccagggcg
     1741 tcgtcttcta taccggtcag ttctatgccc agtccttctt ggaaaatgtt tgtaagatcg
     1801 attttgatca gtcgaagacg atgctgttat tcggcatcct gctggctacg cccttcttcg
     1861 tcgttttcgg gtcctggagc gacaaggtgg gaagaaaatg gatcatgatg atcgggatgc
     1921 tgctggccat ccttacctat cccttcctgt tcaaacaact gctggtccat tcgaaccccg
     1981 aaggccggac ggagttcgcc gaacaaaaag agatcaggac aagcgtcgcc tttatcaata
     2041 caacgagccg cgacgtgatc cgtacctcgg ccaccctctc ccattatgat ggcggcctgc
     2101 tcgtcacgga gacaaaaaag gacacggtct atgcggatgg ccacacgggg aaaaaacccg
     2161 tcgtcaccgt gtcccgtacc ctgggcaccg ccgattactg gaagataacg ggcgtccttt
     2221 tcctcatggt cttttatgtg acgatggtct atggcccgat cgccgccttc ctcgtggaat
     2281 tgttccctac ccgcatccgc tatacctcga tgtccctgcc ctaccatatc ggcaatggcg
     2341 tctttggcgg catgaccccg ttcatcgcca cactgctgac gaccatctac accggcgata
     2401 aactggtcgg tctctggtac cccatcggcg tcaccgccgt ttgcctggtc atcggcgtca
     2461 tctatatccc ggcaaaagga tccaccgaaa catttgatca ataacagcaa taaaaatagt
     2521 agcatcatga acctcctcaa aaaagcgctt ggcatcgtct ggatcgccct gggaccgctg
     2581 gtcctcttct acctcataaa gaccggcgca agcgagatca caaaaaagcc ggtgatcgac
     2641 accaggatcc aatggggggt ctttatcggc atttttatcc ccatcgccgt cgggatcgtc
     2701 ctgttcggct attatgcgct aaagggcgaa tacgggacgg agtcccgtac cccgaagggg
     2761 aataattagc cgcttgacga cgaagggcta tattgtcctt aggcccgcag gaggaaagcc
     2821 gacagcgaat aggccacaaa accctaaatt cgcgtcgtaa acagcgtaaa tgtctattga
     2881 gataaaagac ctcgtgaaaa tatacggcga acaaagggcc gtagacacca tttccttcca
     2941 ggtcggcaaa ggggagatcg tcggcttcct cggtcccaac ggggccggca aatccaccac
     3001 catgaagatc ctcacgggct acctcggcca ggacggcggt gaggcccgcg tctgcggcat
     3061 ctccgtaaag gaaagcccgc tggagacaaa gaaaaagatc ggctatctgc ccgaagccaa
     3121 ccccctctat cccgatatgt atgtccggga atatctcgat ttcatcgccg acgtacaaca
     3181 ggtcaccgac aagaaggcgc gcatcgaagc cgtcatcgcc ctggtggggc tgacggagga
     3241 aagcaaaaaa aagctgggac aactgtccaa aggctataag cagcgggtgg ggcttgccgc
     3301 cgcattgatc cacaaccccg aggtattgat cctggacgag cccaccagcg gtctcgatcc
     3361 gaaccagatc atcgagatcc gcgatgtcat caaacgcctg ggtaaggaca agaccgttct
     3421 cttctcctcg catatcctgc aggaagtgga agccctctgc gatcgcgtgg tcatcatcaa
     3481 caggggaaag ctggtcgccg acagcctgat cagccggctc cgcacagagg gcagtgaagc
     3541 aaacaccatc cgggtcgcct tccgggagca gctggagcct gctgcactcg gggagatcaa
     3601 cggcataagg tccctgcagc ggctttccgc caacgaatgg gaactacaga ccgaagagcc
     3661 cgacgaggtg aaaaaacaat tgctggaact ggctttgcag aacaagtgga acattgtttc
     3721 tttgcaaagc gaaaacaaaa gcctggaagc catcttccgg agcctgacca atcaagatta
     3781 aaaagattaa aatacgaatc catcatgagc tacattgccg acattcatgc aagacagatc
     3841 ttagacagcc gcggaaatcc caccgtcgag gtggacgtcg ttacggaaaa caaccatcgc
     3901 ggccgtgccg ccgtgcccag tggcgcttcc accggcatcc atgaagccgt cgaactccgg
     3961 gatggcgaca aaaaggtcta cagcggaaaa ggtgtattga aagccgtggc gaatgtgaac
     4021 gatatcattg ccgaccaact catcggcttc cccgtgaacg aacaggccag cctggatgcc
     4081 cggctcatcg agctggacgg cacggagaac aaaggcaaac tgggcgccaa tgccctgttg
     4141 gccgtcagca tggccgttgc aaaggccgct gcaaaggaaa gcaagctgcc cctctaccgc
     4201 tacctgggcg gcgtcaacag caccgtattg cccatgcccc tgatgaatat cctcaacggt
     4261 ggtgtccacg ccgataataa gatcgattac caggaatata tgatcgtccc cgtcggcgcc
     4321 gagaccttta gcgacgggct ccaatggggt gtcgagatct tccacacact gaagtccgtg
     4381 ctgaagaaaa aaggctatag caccaacgtg ggtgacgaag gcggcttcgc cccggatatc
     4441 cagagcaacg aagaagccat cgagaccgta ctccaggcca tcgaagccgc cggctacaag
     4501 gtgggcagcc agatcggcat cgccctcgac gccgccagca gcgaaatgtt caaggacggg
     4561 aaatacaaat tctacaagag cagcggaaaa gagctcacca gcgaccagat ggtggagtac
     4621 tggacctcct gggtcaataa atatcccatc atctccatcg aggacggcat ggcggaagag
     4681 gactgggatg gctggaaaaa gctcaccgac gccatcggga acaaggttca gcttgtcggc
     4741 gacgatcttt tcgtcaccaa tacgaagatc ctccagaagg gtatcgaccg cggcgtggcc
     4801 aacagcatcc tgatcaaggt aaaccagatc gggaccatca ccgagacgat taatgccgta
     4861 caattggctc agactcacgg ttataccacg atcatgagcc atcgaagcgg ggagaccgag
     4921 gatacgacca tcgccgacct cgccgtagcg ctcaactgcg gccagatcaa gaccggcagc
     4981 gcgtcccgca ccgaccggct ggccaagtac aaccagcttc tccgcatcga ggaagaactg
     5041 ggagacgccg gtatttatcc caaaggaagg atcaaattca aaaaataagt tgtagattgg
     5101 gtcctgaccc ggctgcgccg ggtcaggacc tttgtttttt tatgaagctg ttgacgcata
     5161 tcccatcctg gttgaagaac aaatacctgt tgaccgccgt
//
LOCUS       DATGFG010000608         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1274629, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000608 DATGFG010000000
VERSION     DATGFG010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274629"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3139
                     /locus_tag="VKQ52_11325"
     CDS             <1..3139
                     /locus_tag="VKQ52_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLZ87828.1"
                     /translation="FLAYLKQRKPKIFDAYDHQQFTFGSLLKRLPIARDPSRIPLVPV
                     VFNIDLGLDNGVAFEGLHYRMAYNPREYENFEIFLNASGSEQALTFEWSYNTQLFKPA
                     SIRRMMEGFEHLLRELTTNTNTLIRDLPVMDSYARNDRLDSLNDTAVAYPKDTPLYAL
                     IDKKAAETPDHPALQFQSIVITYKTLAEKTSRLAHLLKANGVRKGDPVGLALDRSAEM
                     VIALLAIWKAGGAYIPLDPAYPDERIEFMLKDAAAGILLTSGKYRSRFRTQAKNLILE
                     DCWNKVNDYPATPPTTGTGADLAYILYTSGSTGTPKGVAIEHHNLVNFMLSMQRKPGI
                     TAADRLLAVTTISFDIAGLELYLPLISGATLVLADAGSAKDGRILLDIVRTQKITMMQ
                     ATPSTWRMMLDAGWDTPLPLKALCGGEGLPKDLAGTLLTKCSSLWNMYGPTETTIWST
                     VKQIQPDDETITIGRPIDNTQVYILDESATPVALGAIGEIYIGGEGVARGYHNRPALT
                     TERFVNNSFTADGSKMYRTGDLGRLLETGDILCLGRIDHQVKIRGHRIELGEIEHSLI
                     KQPGVKEAVVIAREDRPGDQRLVAYIVPTGAGLPEGSHIPVQQQREWENGLHNLLPAY
                     MIPNDYVAMKLLPLTANGKIDRKSLPRPAAKTGTAEAKEYTPPTTDLEKIIAASWVQS
                     LGLSKVSIHDDFFELGGHSMIAIQVMTQLEKALGTRLPLTTLVECPTIAQLAQRLEQK
                     DNALSWSSLVPIRAEGSKTPLYIVHGGGLTVMIFHSLAKSLDPDQPVYGLQARGLNGI
                     DEPLDNMNAIAAHYLSEILEHNPDGPYCLAGYSFGGFVAFEMARQLLAMGKEVKMLGI
                     FDTAADNTSVQFEPLWKRVLRKIGRQFPKLFFILQSLGRHPGQTIKYQTTFFRKKMRQ
                     PLQRFGLLAEEKNELDTLEHAGKINEKIEIAYSNYKMQPLNINVDLFRVKTRLYFVSD
                     PECLSWRPYALKGVTVHEVPGDHKTFLMDPHYKEMAVILQQCLDERNTAPRIEQKEAI
                     SIRPLVVTRGKLTGT"
     gene            complement(3126..>3309)
                     /locus_tag="VKQ52_11330"
     CDS             complement(3126..>3309)
                     /locus_tag="VKQ52_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87829.1"
                     /translation="YHNNGFEQINTMRFSLDFLPLLFVLAVLGQKDIPAWLMKGMIGY
                     AVFLNLLSFLIHFMYQ"
BASE COUNT          821 a         1155 c          775 g          558 t
ORIGIN      
        1 cttcctcgcc tatctcaaac aacgcaagcc gaagatattc gatgcctacg accatcagca
       61 gttcaccttc ggcagcctcc tgaaacggct gcccatcgcc cgcgacccat cccgcatccc
      121 cctcgtgccc gtcgtcttta atatcgacct gggcctggat aacggcgtgg ccttcgaagg
      181 cctccattac cggatggcct ataacccccg cgaatacgag aacttcgaga tcttcctcaa
      241 cgcctcgggc tccgaacagg cgctgacctt cgaatggtcc tataacaccc aactctttaa
      301 accggcctcc atccgccgta tgatggaagg cttcgaacac cttctccggg aactgaccac
      361 caatacaaac accctcatca gggacctccc ggtaatggat tcctacgccc gcaatgacag
      421 gctcgacagc ctgaacgaca ccgccgtagc ctatccaaag gatacccccc tctatgcgct
      481 tatcgacaaa aaagccgcgg aaaccccgga ccacccggcc ctgcagttcc agagcatcgt
      541 catcacctat aaaaccctgg ccgaaaagac cagccgcctc gcccacctgc taaaagccaa
      601 tggcgtccgc aaaggcgatc ccgtaggcct ggccctcgat cgctcggcag agatggtcat
      661 cgccctcctc gctatctgga aggcaggcgg ggcctatatc cccctcgatc ccgcctaccc
      721 cgacgaacgg atcgaattta tgctcaagga cgcagccgcc ggcatcctgc tcacctcggg
      781 aaaataccgc agccgcttcc gcacacaggc aaagaacctg atcctcgaag actgctggaa
      841 taaagtgaac gattacccgg ctaccccacc cactaccggc acaggggcag acctggccta
      901 tatcctctat acctcgggct ccaccggcac gccaaaaggc gtggccatcg agcaccacaa
      961 cctggtcaac ttcatgctca gcatgcagcg caaacccggc atcaccgccg ccgatcgcct
     1021 gctcgccgtt acgactatct ccttcgacat cgccggtctg gaactctacc tgcccctcat
     1081 cagcggcgcc acactcgtac tggcagacgc cgggtcggca aaggacggcc gcatcctgct
     1141 ggacatcgtc cgtacacaaa agatcaccat gatgcaggcc accccctcca cctggcggat
     1201 gatgctggat gccggctggg atacccccct gcccctgaaa gcgctctgcg gcggcgaagg
     1261 cctgccgaaa gacctggccg gaaccctgct cacaaaatgc tcgtccctct ggaatatgta
     1321 cggccccacc gagaccacca tctggtccac cgtaaaacag atccagcccg acgacgaaac
     1381 catcaccatc ggccggccga ttgacaatac ccaggtctat atcctcgacg aatcggcaac
     1441 ccccgtggcc ctcggcgcca tcggcgagat ctatatcggc ggagaaggcg tagcccgcgg
     1501 ctatcataac cgccccgctc tcaccaccga acgcttcgtc aacaactcct tcaccgcgga
     1561 cggctcgaaa atgtaccgca ccggcgacct cggacgcctc ctcgagaccg gagatatcct
     1621 ctgcctcggc cgcatcgatc accaggtaaa gatccgggga caccggatcg aactgggcga
     1681 gatagaacac agcctcatca aacaacccgg agtaaaggaa gccgtcgtca tcgcccgcga
     1741 agaccgcccc ggcgatcaac ggctcgtagc ctatatcgtt cccaccggtg ccggactccc
     1801 cgaaggcagc cacattcccg tacagcaaca gcgcgaatgg gaaaatggcc tgcacaacct
     1861 gctgccggcc tatatgatcc cgaacgacta cgtcgccatg aaactgctgc cgctgacggc
     1921 aaacgggaaa atagaccgca aatccctgcc ccgccccgcc gccaaaacag gcaccgccga
     1981 ggcaaaagaa tatactccgc cgacaaccga cctggaaaag atcatcgccg cctcctgggt
     2041 ccaatccctc ggcctgagca aggtcagcat ccacgacgat ttcttcgaac tgggtggcca
     2101 ctccatgatc gccatccagg tgatgaccca attggaaaag gcgctgggca cccgcctgcc
     2161 cctgacaacc ctcgtcgaat gccccactat cgcccagctg gcacaacggc tcgaacaaaa
     2221 agataacgcc ctctcctgga gctccctcgt cccgatccgc gccgaaggct ccaaaacccc
     2281 gctgtatatc gtccacggcg gagggctgac cgtgatgatc ttccattcac tggcaaaaag
     2341 cctggacccc gaccaacccg tttatggcct gcaggccagg ggactcaatg gcatcgacga
     2401 accgctcgac aatatgaacg ccatcgccgc ccactatctc tccgagatcc tggaacataa
     2461 ccccgacggc ccctattgcc tcgccggtta ctccttcggc ggcttcgtcg ccttcgaaat
     2521 ggccaggcag ctcctggcca tggggaaaga agtaaaaatg ctcggcatct tcgacaccgc
     2581 cgccgacaac acctcggtac aattcgaacc gttatggaag agggtcctgc ggaagatcgg
     2641 ccgccagttc ccaaaactct tcttcatcct ccaatccctg ggtagacacc ccggccagac
     2701 gataaaatac cagaccacct tcttccggaa gaaaatgcgc caacccctcc aacggtttgg
     2761 attactcgcc gaagaaaaga acgagctgga taccctcgaa cacgccggta agatcaatga
     2821 aaagatcgag atcgcctata gcaactacaa aatgcaaccc ctgaatatca acgtcgatct
     2881 gttcagggta aaaacccgcc tgtatttcgt aagcgacccc gaatgcctca gctggcggcc
     2941 ctatgccctc aaaggcgtga ccgtccacga agttcccggc gaccataaga ccttcctgat
     3001 ggacccgcac tataaggaaa tggccgtgat cctgcagcaa tgcctcgatg aacgcaatac
     3061 agccccgcga atagaacaaa aagaggccat ctccatccgt cccctcgtcg ttacccgcgg
     3121 gaaactcact ggtacataaa atggatcagg aaactgagaa gattcaaaaa gacggcatac
     3181 ccgatcatcc ccttcataag ccaggcgggg atgtcctttt gcccaagaac ggcaaggaca
     3241 aacagcaacg gaaggaaatc gagggagaat cgcatcgtgt ttatctgctc gaacccgttg
     3301 ttatgatag
//
LOCUS       DATGFG010000609         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_176132, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000609 DATGFG010000000
VERSION     DATGFG010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176132"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VKQ52_11335"
     CDS             complement(<1..575)
                     /locus_tag="VKQ52_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87830.1"
                     /translation="MKKTIVFFVLLFLSAGSRLASQTLYVDAVNGNDHAKGTLSHPVA
                     SLEQAVALAKRSAHHEPVTIKIAPGLYKLKRQLKIEPRNGPEDTAEYTFEAMIMPDDT
                     SWKPAKMPVIQSWSTNNTRTFFSHAVGFEILRDNVSIKGLKFVGNADPAVDYYYPIER
                     DSNKLKNLDISQCYFIGDRNSAPIQGAVYTQG"
     gene            complement(597..>2204)
                     /locus_tag="VKQ52_11340"
     CDS             complement(597..>2204)
                     /locus_tag="VKQ52_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLZ87831.1"
                     /translation="STTRYAAPRNELEAALAEIWQQLLAVERVGIHDNFFKLGGHSLL
                     AARVISLIGKTLGAELLIKDLFLYPTLLELAAHLRLQKKGLRLPPITRETGPEHLPLS
                     FSQERLWLTGQAQGSMPHPISRVLRLSGRLDKEALSAALRQTINRHEVLRTVIRAKGD
                     GYCQQVMPADSWQMECIDAQVYRPFDLSADVLLRAQLIAVADQEHLLILVLHPIAADE
                     GSCDILLRELAILYPALTILYPGDDPRAIDILPPLPIQYADYALWQRRRLADGLFSPA
                     LDYWRNELAGVTSFELPTDHARPAVRSVRGASLYLPLDPALLKAARDLARSSGATLFM
                     TFLAAFKILLYRCSGEEDICVGTPVPGRQQPETEGLIGHFINILPLRSRVTADATFLS
                     LLEEVKETTLKAYEHQDVPFEKPAEMGMNERDMPPAPIFRVLFGMQHGSRATEMRIGE
                     LVLSDESSGDTLVNFNLTLTISCGDNAPGCRIAYHTDLYKAETIRRLSSRYMRLLQSI
                     IGAPDRRIAALEDDEEAKVAPLALSHE"
BASE COUNT          465 a          589 c          664 g          486 t
ORIGIN      
        1 ccctgtgtat acaccgcccc ctgtatgggc gctgaattcc tgtcaccgat gaaatagcac
       61 tgggatatat ccaggttctt tagcttgtta ctgtcccttt caatgggata ataatagtct
      121 accgccgggt cggcattacc cacgaacttg agccctttga tagatacatt gtccctgagg
      181 atctcaaacc ctacggcatg actaaaaaag gtgcgggtat tgttcgtcga ccacgattgg
      241 atcaccggca ttttagccgg tttccagctg gtgtcgtccg gcatgatcat cgcttcaaag
      301 gtatattccg ccgtatcctc cgggccgttt cgcggttcga ttttcagctg acgtttgagt
      361 ttatacaaac cgggagcgat ctttatggta accggttcat gatgggcaga cctttttgcc
      421 agggctaccg cttgctccag gctggccacc gggtgagaca gggtgccctt tgcatgatca
      481 tttccattca ccgcatcgac gtacaacgtc tgcgaggcaa ggcggctgcc tgcactcaga
      541 aagagaagga cgaaaaagac gatcgttttt ttcatttatt ttgggagttg agtgaattat
      601 tcatgggaaa gcgcaagtgg cgccactttg gcttcttcgt cgtcctccag cgctgcgatc
      661 cgccggtcgg gggctccgat gatggactgg agcaaccgca tataccgcga cgatagccgt
      721 ctgatcgttt ccgccttgta taggtccgta tggtaggcaa tccggcagcc aggggcattg
      781 tccccgcaac tgatcgttag ggtaagattg aaattcacca gggtgtcgcc ggaagactca
      841 tcggacagga cgagttctcc tattcgcatc tccgttgcgc gcgaaccgtg ctgcatcccg
      901 aacagtaccc ggaatatcgg cgcagggggc atgtcccgtt cattcatccc catctcagcc
      961 ggcttttcaa atgggacatc ctggtgttcg taagctttca gcgtcgtttc cttcacttcc
     1021 tccagcaggg aaaggaaggt agcatctgcg gttaccctgc tgcgcagagg caggatattg
     1081 ataaaatgac cgatcagccc ttcggtttcc ggttgttgcc ggccggggac gggagtccct
     1141 acacagatgt cctcctcacc gctgcaacga tacaataaga tcttgaaggc ggccagaaag
     1201 gtcatgaaca gggtggcgcc ggatgagcgg gccagatccc gtgctgcctt caggagcgcg
     1261 gggtcgaggg ggaggtacag actggctccg cggacactcc gcacggcggg ccgggcatga
     1321 tcggtgggaa gttcaaatga ggtcacaccg gccagttcgt tgcgccaata gtccaacgca
     1381 ggagaaaaga gcccgtctgc gagccggcgg cgctgccaga gagcataatc ggcgtattgg
     1441 atcggtaagg gaggcaggat gtcgatggcc cggggatcat cgcccggata aagaatggtc
     1501 agcgccggat acaggatggc taactcacgc aggagaatat cgcaggaccc ctcatcggcg
     1561 gcgataggat gaagcaccag gatgaggaga tgctcctggt cggcgaccgc gatcagttga
     1621 gccctgagca ggacgtcggc cgaaagatcg aaagggcggt agacctgcgc gtcgatacat
     1681 tccatttgcc agctatcggc gggcatcact tgctgacagt acccgtctcc ctttgcacgg
     1741 atcaccgttc gcagcacctc atgacgattg atcgtctgcc ggagggcagc cgatagcgcc
     1801 tctttgtcca gccttccgct gaggcgcaat accctggaga tgggatgagg catgctgccc
     1861 tgggcctgac cggttaacca caatcgctcc tggctaaagg ataatgggag gtgttccggc
     1921 ccggtttcgc gggtgatggg cggaagccgg aggccttttt tttgcagccg tagatgggcg
     1981 gccagctcta acagtgttgg atataggaaa aggtccttga tcaggagttc ggcgccgagg
     2041 gtcttgccga tcagggagat gacacgggcg gctaataaag aatggccgcc aagtttgaag
     2101 aagttatcat ggatgccgac ccgttccacg gcgaggagct gctgccagat ctcggccagg
     2161 gcagcctcca gctcgttgcg gggcgccgca taccgggttg tgga
//
LOCUS       DATGFG010000610         8008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_916743, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000610 DATGFG010000000
VERSION     DATGFG010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916743"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VKQ52_11345"
     CDS             <1..980
                     /locus_tag="VKQ52_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ87832.1"
                     /translation="QLCNNCLIQLLIPGGGTIQMNYQVVLTGGSFLTVGSGVNLKMIG
                     TNATAFNDPTNPANYIILTGNGNNQIQWADNTATLTATNLGPYDGIFTSTQVNATDFV
                     LLKEFGAAPVQFTNNTITNTGPSIFGTSLVGPNTLNGFGTLPILVSSFSATLNQDVVN
                     LAWTTTLEENADHIAVERSSDAGAHWATLGTVPAKGNSGSATDYTFTDDRLIAGTLEY
                     RLQLVDKDGKYKYSDVKVIRTSLITAANIYPNPAHDYVNVTLGGNSTETIAIRLFNQN
                     GQLLQEKSVSNGGGTTVALSVGNYPQGNYLLVVTAADGTKQTSKLLISK"
     gene            1101..2267
                     /locus_tag="VKQ52_11350"
     CDS             1101..2267
                     /locus_tag="VKQ52_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ87833.1"
                     /translation="MRRRTSSVLKKVGLAMCIGMLSHGNSSAQCINNMGTRTYDTTLT
                     NNGFGNYNLSFPQWSPDSGQLVSVKLTANVTSTYGYNLRNVTASPATYMVNIGQEDQI
                     SAAFGAPFTSLTPQYVGTYTLQAGESQLVAPFPFLTNHISSDSLTGNVATFLGNSSVN
                     INYLSFTYTNLVAYNNASYYYSNTINTTTQFSVQYLYCQAGVVLAANLTRWTATLTAP
                     STVELDFTAVNEVAGRRYEIQRSSDGHSFTTIATQTATSDGLTADYTYTDHLPTGAGG
                     KWYYRLQVVDNGTFSWSAVREIDVNAMEKNLRIYPNPATDHIDIATGTANSDWQTDIL
                     SASGMLVQRETLLNSGLLHIPFRNRLSAGTYFVRITDLRAQKTQVSSFIVNPAN"
     gene            2490..3575
                     /locus_tag="VKQ52_11355"
     CDS             2490..3575
                     /locus_tag="VKQ52_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ87834.1"
                     /translation="MTYDTVLTSIDGNEVYTYNVPYFPVSNLTLYAVTIRSIINVQSG
                     MTLTNISSGTLTASAQWQRTDDISSAPTGDFYNTLNPSAFKATNLAAGASKVFAPTMI
                     ISNNTALVDSVNTADGSLVAGDYEGTGYITFQYTTTSTLSLLPSPTKVDSSASSFADQ
                     MHFMVTYYYCNPGNLATDILNFTAVKQDNGTVLLAWSSANEQPHRDYKIQVSTDGFNF
                     TDYASVASEPVNSSANYTYSYPVDPTAAGKLYFRLRIVDEIGPPHYSIVCIIDLDHGV
                     SVRSGFFIYPNPPTDFVNLNLPGGSPQPWQVDIIAADGSLIQRNRITNTNTPRVDFVR
                     RLSGGTYFVRAFDPRNGTRYSASFIVQ"
     gene            complement(3746..4942)
                     /locus_tag="VKQ52_11360"
     CDS             complement(3746..4942)
                     /locus_tag="VKQ52_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ87835.1"
                     /translation="MKKSNVFAFYALLIGLLCTRSAFVRGQTNLDKGENAIKSFFKKK
                     NKSPQADTARAATGNASATNASATGAAVAGDAGNNAPVSLKTYSNYDFVPGDQIVFED
                     NFADDADGEFPAHWSLEKGQAIINKVGGIQAFFLTEGNYVRVAPRMKTPDNYLPENFT
                     IEFDYFSNHGYDPVILFTTATGDSRHLHFGREVASGYFPKDFSGEYPGDRGDAFDGRW
                     HHAAMIKKGNQIKCYEDQYRVLVMPDCGECKVTKLEMGGIGSLENPIVFKNFRIAAGG
                     NMNMIGKKFTENKIVTHGINFDVDKATIRPESMGTLNMIVGVMKDNPDVKFEVDGHTD
                     NSGAPAHNLDLSQQRADAVRAQLISMGVDASRLTTRGFGDTKPISDNTTPEGKANNRR
                     VEFVKI"
     gene            complement(5074..5703)
                     /locus_tag="VKQ52_11365"
     CDS             complement(5074..5703)
                     /locus_tag="VKQ52_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87836.1"
                     /translation="MIKIAIVEDNNTLRSSLESLFNRTEGMRCVASLNNLLNVISEVG
                     STNPDIILMDIGLPNISGIEGVRTVKTHFPGIQVLMFTVFDDDEKIFDAIRAGASGYL
                     LKKTPPAEIVQAVQDLYHGGAPMTSSIARRVIQSFQAAPSTLVEDYRLTVREDEILYS
                     LVDGLSYKKIADKYCVSISTIRTHICNIYHKLHVNSKAEAVARVLGPKR"
     gene            complement(5766..7652)
                     /locus_tag="VKQ52_11370"
     CDS             complement(5766..7652)
                     /locus_tag="VKQ52_11370"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HLZ87837.1"
                     /translation="MKPAFFLSVWLFVAGLVRGQEAVTNLAAARGLGLEGPVCRVDST
                     IVPYNQFLALLEREPRRHVFGVKVLLRNSSDSLLDLSLGYGEVDYIDAILVMAGGGVQ
                     TGASGDTQTIAGGRLRPTPKGSTYLQRQTEVLPLRLPAHSRAWLTVSIRQRTDEYNFN
                     GLTLYDAGALNAAFVHDLPAGNSFLIIQMLFQGFLLCQLLFVLFQWLIIRRKEYLYYS
                     LYMALIALYFLSKQESLFGMEILFSRWPLLKIYLGKTLLILPYYVYFRFVRSFLEIPR
                     DHPRLNRWMVRIEYFLLGYAVLDLVFIVITFDRGVQTQIYTSVFTLVFLLTLGFLIYM
                     YRHRQALVLFIISGSLFVATGHILGLIFSYLEVNRHIDLGVPDIFIFPQTGIVLEVLC
                     FTAGLSYKGQMTEKEKISSQQKLIEQLKANELLQNRMQHIRNKIAQDLHDDIGSTLSS
                     ISILSDLALRENSSSQTMETMNEIKDSSMLLMDRMDDIVWSINPRNDSLENLLIRVRH
                     FATTLFEARGVDYVIEIQKNINEVRLPMDYRQHIYLILKEAINNLVKYAGASQAWLEV
                     QFDERHLRVCVRDNGRGFDVTVPGTGNGLSGMRRRAELMGAQVSVVSSPGQGTSIGLR
                     VDIG"
     gene            complement(7706..>8008)
                     /locus_tag="VKQ52_11375"
     CDS             complement(7706..>8008)
                     /locus_tag="VKQ52_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ87838.1"
                     /translation="QVEGERVNTAFFRQGETKIELVQGLGPESVISRYIDKRGEGMHH
                     IAFEVADIHAEMRRLAAEGFTLLNAEPKDGADNKLVCFLHPKDTNGVLIELCMERR"
BASE COUNT         1843 a         2469 c         2026 g         1670 t
ORIGIN      
        1 cgcagctgtg taacaactgt ctcatccaac tgttgatccc tggcggcggg acaattcaaa
       61 tgaactacca ggtggtcctc accggcggtt cttttctcac cgtcggcagc ggggtaaacc
      121 tgaaaatgat cgggaccaac gctaccgcct tcaacgatcc gaccaacccg gcaaattaca
      181 tcatcctcac cggcaacggc aacaaccaga tccaatgggc ggataacacg gctacactca
      241 ccgccaccaa cctcgggccc tacgacggta tcttcacgtc cacccaggtg aacgccacgg
      301 atttcgtcct gctgaaagag tttggcgcgg ctcccgtaca attcaccaac aacaccatta
      361 cgaataccgg tccctccatc ttcggcacca gcctggtagg tcccaatacc cttaatggtt
      421 tcggtaccct cccgatcctc gtgtcttcct tctcggctac cctcaaccag gatgtagtca
      481 acctggcctg gaccacgacc ctggaagaga acgccgacca catcgccgtc gaacgcagct
      541 cggacgccgg cgcccattgg gcaaccctcg gcaccgttcc cgccaaaggg aactccggct
      601 ccgccacgga ttacaccttt acggatgaca ggctcatcgc cggcaccctc gaataccggc
      661 tgcaactcgt agacaaggac ggtaagtata aatactccga tgtcaaggtc atacgcacca
      721 gcctgatcac cgcggccaac atctacccca accccgccca tgactatgtc aatgtaaccc
      781 tcggcggcaa ctccaccgag accatcgcca tccgcctctt taaccaaaac ggacaactgc
      841 tccaggaaaa gagcgtaagc aatggcggcg gcaccaccgt tgccctgtcg gtcggcaact
      901 atccgcaagg aaattatctc ctcgtcgtca ccgcggcaga tggaaccaag cagacatcaa
      961 agctcctgat ttccaaatag tctacactct tataacaata gaacgcagaa gccgcctccc
     1021 aatggggggc ggttttgttt tgggataaag aaaataaatc atgagtattt tgagttatta
     1081 ggacaatcca atatccgact atgagacgcc gtacttcgtc cgtactgaaa aaagtgggtc
     1141 tggccatgtg catcggaatg ctgtcgcatg gcaactcttc cgcccaatgt atcaacaaca
     1201 tgggcaccag gacctatgat accaccctga caaacaatgg cttcgggaac tataacctca
     1261 gctttcccca gtggagcccc gactcgggac aattggtttc cgtcaaactc accgccaacg
     1321 tcacctccac ctatggctac aacctccgca acgtaaccgc cagcccggct acctatatgg
     1381 tcaatatcgg ccaggaagat cagatcagcg cagccttcgg cgcccccttc accagcctga
     1441 ccccccaata tgtcggaacg tataccctgc aagcgggaga atcacagctg gtagcgccct
     1501 ttcccttcct taccaaccat atctccagcg acagcctcac cggcaacgta gctaccttcc
     1561 tcggcaacag ttcggtgaac atcaactacc tgtcctttac ctataccaac ctggtggcct
     1621 ataacaacgc ctcttactat tacagcaata cgatcaacac gacgacccag ttctccgtac
     1681 aatacctgta ttgccaagcc ggggtcgtat tagcggctaa ccttacccgt tggaccgcga
     1741 cactcaccgc cccgtccacc gtggaactcg acttcaccgc cgtgaacgaa gtcgccggcc
     1801 gccgctatga gatccagcgg agcagcgatg gccacagctt taccaccatt gcaacacaga
     1861 cggcgacatc cgacggcctg acggccgatt atacctacac cgatcacctg cccaccggcg
     1921 ccggcggaaa atggtactac cggctacagg tagtcgataa tggaaccttc tcctggtcgg
     1981 ccgttcggga gatcgatgtc aatgccatgg aaaaaaacct gcgcatctac cccaatcccg
     2041 cgaccgatca tatcgatatc gccaccggta cggccaatag cgactggcag acggacatcc
     2101 tctcggcaag cggtatgctg gtgcagcgcg agaccctgct caactcgggc ctcctccata
     2161 tccccttccg caacaggttg tccgcgggaa cttacttcgt gcggatcacg gacttgcgcg
     2221 cacaaaagac acaggtctcc tcctttatcg tcaacccggc caactgattc ctatcgaatt
     2281 ttaatccggg ggataaaata aatccctttg gattagagtt attacaacgg aatccctatc
     2341 gcaaaatcga atttgttcct atgaggcggc gaagaatcca cttatccggg gtatccgtaa
     2401 tcctcctgat cttgctgttg tcaggagcat cactgttacc attgcgccta ccggctcaac
     2461 tgatcacctg ttcaggtaca ctgggttcca tgacctatga cacggtgttg acaagcatcg
     2521 acgggaacga ggtctacacc tacaacgtcc cgtatttccc ggtcagtaat ctcacactct
     2581 atgcggtgac catccgctcg atcatcaacg tacagtccgg tatgacgctg accaatatca
     2641 gcagcggcac gctcaccgct tccgcacaat ggcaacggac agacgacatc agcagcgccc
     2701 ccacggggga tttctacaat accctcaacc cttcagcctt caaggccacc aacctcgctg
     2761 cgggggcatc gaaagtgttc gccccgacga tgatcatcag taataacacc gccctcgtag
     2821 actccgtcaa caccgcggat ggtagcctgg tcgcgggcga ctacgaaggc acaggctata
     2881 tcacctttca gtatacgacc accagcacac tgtccctcct gcccagcccc acaaaggtgg
     2941 acagctcagc gagcagcttt gccgaccaga tgcatttcat ggtcacctac tattattgca
     3001 accccggcaa cctggccacc gacatcctga acttcaccgc ggtgaaacag gataatggca
     3061 cggtcctgct cgcctggagt tccgccaacg aacaacccca ccgggattac aagatccagg
     3121 taagcaccga cgggttcaac ttcaccgact acgcttccgt cgcctccgaa ccggtcaaca
     3181 gctccgcaaa ctatacctat tcctatcccg tcgatcccac tgccgccggt aaactctatt
     3241 tccgcctgcg catcgtggat gagataggcc ctccacatta ttccatcgtt tgtatcatcg
     3301 atctcgatca cggcgtctcc gtgcgaagcg ggttctttat ctatcccaac ccgcctaccg
     3361 atttcgtcaa cctgaatctc cccggtggta gcccgcagcc ctggcaggtg gacatcatcg
     3421 ccgccgacgg cagcctcatc caacgtaatc gcattaccaa cacgaatacc ccccgcgtgg
     3481 actttgtccg gagactatcc ggcggcacct attttgtccg ggccttcgac ccccgcaacg
     3541 gaacacgcta tagcgcatcc tttatcgttc agtaaaaaaa gaaccgccgg cccgggcctg
     3601 tctccggacg cggcggtagc ggcctgcatc gaaatattgc cgctccgccg cgtcggctaa
     3661 aaatggccct tcgggcatgg gagctgtgct cccatctgcg gcggtttgtc acacctgccg
     3721 ggagcctaac tcccgtctgc tatcattata tctttacgaa ctcgacgcgg cggttgttgg
     3781 ccttgccttc aggcgtcgta ttatccgaga taggtttggt gtcgccaaag cccctggtgg
     3841 taagcctcga agcatcgaca cccatggaga tgagctgggc cctgacggca tcggcgcgct
     3901 gctgggaaag atcgaggtta tgcgccggag cgccgctatt atcggtgtgc ccatccacct
     3961 cgaatttgac atcgggattg tccttcatca ccccgacgat catattcaac gtacccatgc
     4021 tctcgggcct gatcgtggcc ttgtcgacat cgaagttgat gccatgggtg acgatcttgt
     4081 tctcggtgaa cttcttcccg atcatattca tattgccgcc ggcggcgatg cggaagttct
     4141 tgaagacgat cgggttctcc aggctgccga tgccgcccat ctccagtttg gtgaccttgc
     4201 attcgccaca gtcgggcatg acgagcaccc ggtactggtc ctcataacac ttgatctggt
     4261 tccccttctt gatcatggcg gcatgatgcc acctgccgtc gaaggcatcc ccgcggtcgc
     4321 cgggatattc accgctgaag tccttgggga aatagcccga ggctacctcc ctgccgaagt
     4381 ggagatgccg ggaatccccg gtggcggtcg tgaacaggat caccgggtca tatccatggt
     4441 tggagaaata gtcgaactcg atggtgaaat tctcgggcag ataattgtcg ggcgttttca
     4501 tccgcggagc cactctgaca taattcccct cggtgaggaa gaaggcctgg ataccaccca
     4561 ccttattgat gatcgcctgg cctttttcca gcgaccagtg cgcggggaac tcgccatcgg
     4621 catcgtcggc gaaattgtcc tcgaacacga tctgatcgcc ggggacgaaa tcataattgg
     4681 aataggtctt caggctgacg ggggcattgt tccccgcatc accggcgact gctgcacccg
     4741 tggccgacgc attcgtagcc gatgcattgc cggttgcggc cctggcggtg tccgcctgcg
     4801 gactcttgtt cttcttttta aaaaagcttt ttatggcgtt ctcccccttg tcgagattgg
     4861 tctgcccccg gacaaaggcc gaccgcgtac aaagcagacc gatcagcagg gcataaaagg
     4921 cgaagacgtt actttttttc atggttgatc tatttgagca aatagacgat aaaaccatgc
     4981 acgggtcaat cggatgaaca tatgattttg gacggccttc ccgcaactgg gcgatcggac
     5041 aactgggcga tcgggcaacc ggggcgatcg gccctaccgc ttcggcccca ggacacgggc
     5101 caccgcctcg gccttcgaat tgacgtggag cttgtggtag atattgcaga tatgcgtgcg
     5161 gatggtgctg atgctgacac agtatttgtc ggcgatcttc ttgtagctaa ggccgtcgac
     5221 aagagagtat aggatctcgt cctcgcggac ggtgagccgg taatcctcga caagggtaga
     5281 gggcgcggcc tggaaggact ggatcacccg ccgggcaata ctcgaggtca tgggggcgcc
     5341 gccatgatag aggtcctgca cggcctggac gatctcggca ggcggggtct tcttgagcag
     5401 gtaaccgctg gcgccggcac ggatggcgtc gaagatcttc tcgtcgtcgt cgaagacggt
     5461 gaacatcagg acctggatgc cggggaaatg ggtcttgacg gtccggaccc cctcgatccc
     5521 ggaaatattg ggcaacccga tatccatcag gatgatgtca gggttggtgc tccccacctc
     5581 gctgatgaca ttcaaaaggt tgttcaggga ggccacgcac cgcataccct cggtccggtt
     5641 gaaaagactt tcgaggctgg agcgcaacgt attgttgtct tctacgatgg caatcttgat
     5701 catcgcgcca aatatgcaaa gtattttgga ggattccaat cggatattta taggatattt
     5761 cggattcatc caatatccac ccgcagccca atggacgtgc cctgcccggg tgacgagacg
     5821 acggacacct gcgcacccat caactccgcc cggcgccgca tccccgaaag gccattgccc
     5881 gtgccgggaa cagtgacatc gaagccccgg ccattgtccc gcacgcaaac ccggagatgc
     5941 cgctcgtcga actgcacctc cagccaggcc tgcgaagccc cggcatattt aaccaggtta
     6001 ttgatagcct ccttgaggat gagataaata tgctggcgat agtccatcgg cagccgtacc
     6061 tcgttgatat tcttctggat ctcgatgaca taatccacac ccctggcctc gaagagcgta
     6121 gtggcgaaat gccgcacccg tatcagcagg ttctccaggg agtcgttgcg ggggttgatg
     6181 ctccacacaa tatcgtccat ccggtccatc aacagcatcg agctgtcctt gatctcgttc
     6241 atggtctcca tcgtctggct gctgctattc tcccgcaaag ccagatcgct gaggatggag
     6301 atgctgctga gggtggagcc gatatcgtca tggagatcct gcgcgatctt gttccggata
     6361 tgctgcatcc tgttttgcag cagctcgttc gcctttagct gctcgatcag cttttgctgg
     6421 ctgctgatct tttccttttc ggtcatctgg cccttatagc tgagaccggc cgtaaaacaa
     6481 agcacttcca ggacgatccc cgtctggggg aagataaaga tatcgggcac cccgaggtcg
     6541 atatgccggt tgacctcgag atagctgaag ataagcccga ggatatgacc cgtggccaca
     6601 aaaagactgc ccgagatgat aaataacacc aacgcctgcc ggtgccggta catatagatc
     6661 aggaaaccca gggtgagcag gaacaccagc gtgaaaacac tggtatagat ctgcgtctgg
     6721 acgccccggt caaaagtaat gacgataaag acaagatcga ggacggcata gccaagcagg
     6781 aaatattcga tccttaccat ccagcggttc agccggggat gatcacgggg tatttccaaa
     6841 aagctgcgga caaatcggaa atagacatag tacggcagga tcagcagggt cttgcccagg
     6901 tagatcttca acaacggcca gcgggagaaa aggatctcca tcccaaagag cgattcctgt
     6961 ttgctcagga aatacagggc gataagcgcc atgtaaagag aataatagag atattccttg
     7021 cgacggatga tcagccattg gaatagcacg aacagcaact ggcacaggag gaagccctgg
     7081 aaaagcatct ggatgatgag gaaggagttg cccgccggca ggtcgtggac aaaggcggca
     7141 ttgagggctc ccgcgtcata gagggtgagc ccgttgaaat tgtattcgtc ggttcgctgc
     7201 cggatgctga cggtgagcca tgccctgctg tgcgcgggaa gacgcagggg aagaacctcg
     7261 gtctggcgtt gcaaataagt actccccttc ggcgtagggc gtaatctccc gcccgcaatg
     7321 gtctgggtgt caccactcgc tccggtctgc acacccccac ccgccatcac gagaatagca
     7381 tcgatataat ccacctcccc gtagcccagg gaaagatcga gcagcgagtc gctgctgttc
     7441 cgtaatagca cctttacacc aaacacatgc cggcgcggtt cacgctcgag taaggccagg
     7501 aattgattgt agggaacaat agtggagtcg acacggcaca ccggcccctc cagccccagg
     7561 cccctcgccg cggccagatt ggtgacagcc tcctggcccc tgacaaggcc ggcgacaaac
     7621 agccaaacgg acaagaagaa cgcaggtttc acggtcagtt tgatttttag gctaaggaac
     7681 gccaccaatc caaggggcac tcctctcagc gccgttccat acacagctcg atcagcaccc
     7741 cattggtgtc cttcggatgc agaaaacaca ccaacttgtt gtccgcccca tccttcggct
     7801 cggcattcag cagcgtaaaa ccctccgccg cgagccgccg catctcggcg tggatatccg
     7861 cgacctcaaa ggcgatatga tgcatccctt ccccgcgttt gtcgatatag cggctgatga
     7921 cactctcggg ccccagcccc tgcaccagtt cgatcttcgt ctccccttgc cggaaaaagg
     7981 ccgtattcac ccgttcgccc tccacctg
//
LOCUS       DATGFG010000611         3897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1205516, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000611 DATGFG010000000
VERSION     DATGFG010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205516"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VKQ52_11380"
     CDS             <1..479
                     /locus_tag="VKQ52_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87839.1"
                     /translation="TTENRARVVPETTASKPGDTTATSGDSTPAGGAASAATSTSGAA
                     KDSVANTGGQAVAKPAATTPVPPAVSPAPIVGRDPVPVAGQSLYHFVILRTESKNHAL
                     RRYNQLLGYQLNIRLETKDSTFFKLYFPIAAAIRDTAHIRDSLADVYAAKISIESH"
     gene            495..998
                     /locus_tag="VKQ52_11385"
     CDS             495..998
                     /locus_tag="VKQ52_11385"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLZ87840.1"
                     /translation="MRPSASYWIEKLQLTPHVEGGAFGEVYRSELIIPRSSLPLFFQG
                     SRNASTSIYFLLAAGQFSAFHRIASDELWHFYFGDPLLVYEITHGGRLIEHRLGQNPE
                     NGESFQTVVKAGSWFASVPAPGSEYTLVGCTVAPGFDFEDFELAEREPLASQYPEHAE
                     IIRRLTR"
     gene            complement(1036..2328)
                     /locus_tag="VKQ52_11390"
     CDS             complement(1036..2328)
                     /locus_tag="VKQ52_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ87841.1"
                     /translation="MAAVILSFALLFTTPAHAQFPADTMRHTYLLRPDRVFDGEQMHE
                     GWQVLVHEHRIEAIGPAADIKAPAGAITLDLHGATLLPGLIEGHSHLFLHPYNEVAWD
                     DQVLKEPRAERTARAVVHARQTLLAGFTTTRDLGTEGAWYDDVGLKESIEKGIIPGPR
                     MLCATRAIVATGSYGPKQLSYDLESPHGAAEADGPEGVTKEVRNQIAHGASLIKVYAD
                     YRWGLDGTAQPTFTVEELKTIVEVAASSGRQVIVHSSTTEGMRRAIEAGVSTIEHGEG
                     GNPELFAAMKAKGIALCPTLAAGEAILQYKGWRKGTDPDPERIKAKKENFREALASGV
                     TICMGGDVGVYTHGTNAREMEDMVEYGMKPLDVLRSATSVNADVFRIGNHVGRIKPGL
                     LADLVVVQGDPLTNMTYIRKIVWVMKDGVIYRPDPVTP"
     gene            complement(2576..>3897)
                     /locus_tag="VKQ52_11395"
     CDS             complement(2576..>3897)
                     /locus_tag="VKQ52_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLZ87842.1"
                     /translation="SPYMLLLVWVVAGAVSLLGAMIYAELGTMFPDTGGPYIYLQKTY
                     GDFTAFIYGWSASVVINTTALASMAFVAAQYIGFFVHLPRFDPAVEASIKLHLPFVAD
                     IYPLENFGVKSTAAAILLGLTVLNYCSTKQSNIVQLLATALKTLALVLLIGGLLFTGK
                     GNTANFVTNSPDFHPQNILFLLAAFMAATSGAFSSYDGWYNLNMVAGEIENPGRNISR
                     SLLIGLGSCIGIYALVTLAYIYVIPVGQMAHSSLVAADAMEKVLGVAAAGFVSVLIVI
                     SAFGATNANFLANIRVLFAMGEGGDFFAWGGRVHPRYGTPGNAAVFMGVISIGFVYSG
                     SFDILADMFVFLSWIFYGLTAVGLFILRKRMPDHPRPFRVKGYPILPAIFIAFAAFYL
                     VTTIYNDISGYNSGKSPIIESVFGLLLTATGIPFYLYFQRQKRKNEK"
BASE COUNT          800 a         1055 c         1292 g          750 t
ORIGIN      
        1 ccaccacaga gaaccgggcc agagtagtcc ccgagaccac cgcgtcgaaa cccggggaca
       61 cgacggcgac aagcggagac agcacgcccg cgggtggagc ggcgagcgca gcaacgtcta
      121 caagcggtgc ggctaaagac agtgtggcga atacgggtgg ccaggcggtg gcaaagcccg
      181 cggccacgac gcccgtccct ccggcagtaa gcccggcgcc catcgtcgga cgggatcccg
      241 tcccggtagc aggacaatcc ctttaccatt tcgtcatcct gcggaccgag agcaagaacc
      301 atgcgcttcg cagatacaat caattacttg gttaccagtt gaatatccgc ttggaaacaa
      361 aggattccac ctttttcaag ctctatttcc cgattgccgc ggcgataaga gataccgcgc
      421 atatccggga ctcgctggcc gatgtctacg ccgcgaaaat atcgatcgaa tcccattgac
      481 ctcttattaa tatcatgcga ccttccgcct cgtactggat cgaaaaattg caattgaccc
      541 cgcacgtcga aggcggcgca tttggcgaag tgtatcgcag cgaactgatc atcccccgga
      601 gttccctacc cctctttttc cagggcagcc gcaacgcctc gaccagtatc tattttctgc
      661 tcgccgcggg ccagttctcg gcctttcacc ggatcgcctc agatgagcta tggcattttt
      721 attttggaga cccgctgctg gtgtacgaga tcacccacgg cggccgcctt atcgagcatc
      781 gccttggcca gaacccggaa aatggcgagt cgttccagac ggtggtaaaa gcggggagct
      841 ggtttgcgtc cgttccggcc cccggcagcg agtacaccct tgtcgggtgc acggtggcgc
      901 cgggttttga ttttgaggat tttgagttgg cggagcggga gccgctggcg tcgcagtacc
      961 cggagcatgc ggaaattatc aggagattga cgaggtgagg cggccggcac catggtttgt
     1021 ggtgacgccg gccgcctatg gtgttacggg atcgggacgg tagatgaccc catccttcat
     1081 tacccaaacg attttacgga tgtacgtcat gtttgtgagg ggatcaccct gcaccacgac
     1141 gaggtcggcg aggagaccgg gttttatccg gccgacatgg ttaccaatgc gaaagacgtc
     1201 ggcattgacg gatgtagcgg accgcaggac gtcaaggggt ttcattccat attccaccat
     1261 gtcctccatc tcgcgggcat tcgtcccatg cgtatacacc ccgacatcgc cgcccatgca
     1321 gatggtcacc cccgaagcga gggcctcacg gaaattctcc ttttttgcct taatgcgctc
     1381 aggatcgggg tccgtgccct ttcgccagcc tttatactgc aggatggcct cgcccgcggc
     1441 cagggtcgga cagagcgcga tgccctttgc cttcatcgcg gcgaagagct cgggattacc
     1501 accctcccca tgttcgatcg tgctgacgcc ggcttcaatc gcgcggcgca tgccttcggt
     1561 tgtcgacgaa tgaacgatga cttgccggcc gctgctggcg gctacttcca cgatggtctt
     1621 caactcctcc accgtgaatg tcggctgggc cgtgccgtcg agaccccagc ggtagtcggc
     1681 gtagactttt atcagagagg cgccatgggc gatctggttg cgaacttcct tcgtgacgcc
     1741 ctcggggccg tcggcttcgg ctgcgccatg gggtgattcg aggtcgtagg ataactgttt
     1801 cggtccgtag ctacctgtcg ccacgatggc ccttgttgcg cagagcatgc gcggaccggg
     1861 gatgatgccc ttttcgatag actccttcag cccgacgtca tcataccagg ccccttcggt
     1921 acccagatcg cgggtggtgg tgaagccggc gagcaaggtc tggcgggcat ggacgacggc
     1981 gcgggcggtg cgttcggcac ggggctcttt caacacctgg tcgtcccagg ctacctcatt
     2041 atacggatgc aagaagagat gactatgccc ttcgatgagg ccggggagca gtgtcgcgcc
     2101 atggaggtcg agggtaatcg cgccggcggg agcctttatg tcggcggcgg ggccgatcgc
     2161 ttcgatgcgg tgttcatgga cgaggacctg ccagccttcg tgcatttgtt cgccgtcgaa
     2221 aacgcggtcg ggccgcagga ggtaggtgtg gcgcatggtg tcggcgggga attgggcatg
     2281 cgccggagtg gtgaagagga gagcgaacga gaggatgacg gctgccacaa ttatgaaggt
     2341 cggtaaggga tgtaaccgag cgcagcgaag gttaggaacg agaagaagcg agtgcccgga
     2401 gcttcgacga agttcggtct gcgcggcgga ggatggtggt ctggcggatt gcgttaggga
     2461 tcgaaggacg taccgtgtac gccggagagc ccgacccgcg gtcgcgggga acgcccaagg
     2521 catcctgaca ccggccggtt gttgccgggc gcgaggtgtg gagtcgcggt gcaggttatt
     2581 tctcattttt ccgcttttgc cgttgaaagt acaaataaaa aggaataccc gtagcggtga
     2641 gcaggaggcc gaagaccgat tcgatgatgg ggctcttacc gctgttatag ccgctgatat
     2701 cgttgtaaat ggttgtgacg agatagaagg ccgcgaaggc gatgaagatc gcagggagga
     2761 tggggtagcc ttttacgcgg aacggccggg ggtggtccgg cattcttttg cgcaggatga
     2821 agagaccgac cgcggtgagg ccatagaaga tccaggagag gaagacgaac atatcggcaa
     2881 ggatgtcgaa gctgccgctg tatacgaagc cgatgctgat cacgcccatg aagacggcgg
     2941 cgttgccggg ggtgccatag cggggatgga ctctcccacc ccaggcgaag aagtcgcccc
     3001 cctcccccat cgcgaagagg acgcggatgt tggcgaggaa attggcattc gtggcgccaa
     3061 aggcggagat gacgatcagc accgagacga acccggccgc agccaccccg aggacctttt
     3121 ccatcgcgtc ggcggcaacg agggaggagt gggccatctg cccaacggga atgacataga
     3181 tgtacgcgag ggtcaccagg gcgtagatac cgatacagga cccaagcccg atgaggaggc
     3241 tgcggctgat gtttcggccc gggttctcga tctcgcccgc gaccatattc agattatacc
     3301 acccgtcata cgaggagaag gccccggagg tggcggccat gaaggcggcc aggaggaaga
     3361 ggatgttttg cggatgaaag tccgggctgt tggtgacgaa gttggccgtg ttacccttgc
     3421 cggtgaagag gagtcctccg atgagcagga caagtgccag ggtcttgagg gccgtagcga
     3481 gaagctgaac gatattgctt tgcttcgtgc tgcaatagtt gagtacggtg aggccgagga
     3541 ggatggcggc ggcggtggat tttacgccga aattctccag gggatagata tcggcgacga
     3601 agggaagatg gagctttatc gaggcttcga cggcggggtc gaagcgcggc aggtggacga
     3661 agaaaccaat atattgagcg gcgacgaagg ccatggaggc aagcgcggtg gtgttgatga
     3721 cgacggaggc ggaccagcca tagatgaagg cggtgaagtc gccgtaggtc ttttgcaggt
     3781 agatataggg gccgccggtg tcggggaaca tcgtccccag ttcggcatag atcatggcgc
     3841 cgagcaggga gaccgcgccg gcgacgaccc agacgaggag gagcatgtac ggcgaac
//
LOCUS       DATGFG010000612         8349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_608900, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000612 DATGFG010000000
VERSION     DATGFG010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608900"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VKQ52_11400"
     CDS             <1..209
                     /locus_tag="VKQ52_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HLZ87843.1"
                     /translation="VAGGVGIAGMQTGIYTLNSPGGWQIIGRTPVRLFDPQADPPIRL
                     QPGDRVRFYPVTPTEFRELSGRPG"
     gene            343..1338
                     /locus_tag="VKQ52_11405"
     CDS             343..1338
                     /locus_tag="VKQ52_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HLZ87844.1"
                     /translation="MAFSIIKPGLLDTIQDLGRHGFGNWGVSPGGAMDSYAAQVANLL
                     VGNDKQEAVMEIHFPGPQILFEQNTLIAITGADFSPTVNDEVIPRWQPVVVRKNTVLH
                     FPSLVQGGRCYLSVHGGYCLKKWLNSYSTNLKAGAGGWHGLALKKGDELPFNENTIYF
                     AGLLKDESNYEVLPWRVNPDKIYQYPHEIGFIPGHEWGLLSAASKSVFLENNFLIHPS
                     SDRMGYQLKGAPLALDQPVELVSSAVNFGTVQLLPNGQLIVLMADHQTTGGYPRIAHV
                     VSAHLPKLAQLRPSDTIQFKLMELETAERMVISRQKELHILQRSCMDHLNELVCQ"
     gene            1329..2099
                     /gene="pxpA"
                     /locus_tag="VKQ52_11410"
     CDS             1329..2099
                     /gene="pxpA"
                     /locus_tag="VKQ52_11410"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HLZ87845.1"
                     /translation="MPVDLNCDMGEGMDTDAAIFPFITSTNIACGAHAGDGDTMRRTV
                     ELAMVQGVAIGAHPSYPDRARFGRVDILGAGLSPAALKDSLAALEDSLFEQLRSLDAV
                     CRELGVRMHHVKPHGALYNRAARDVLVSGLICRVVAAVDASLLLYGLSGSGMSAAAAE
                     AGLGFVSEVFADRGYRPDGSLVPRSEPGALIEDEAAAVAQVLKMVREGRVAVTGGGEV
                     TLRAETVCLHGDGAHAVEFARLIHGALAAEGVKMVAPA"
     gene            2223..3494
                     /locus_tag="VKQ52_11415"
     CDS             2223..3494
                     /locus_tag="VKQ52_11415"
                     /inference="COORDINATES: protein motif:HMM:NF025720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HLZ87846.1"
                     /translation="MATTTDQSRLEGGYTYDSFTGVSSRAKRPVETLFLWWCSGAYQG
                     LLKDCPSEQTKYAGLGGVLLATFVLAALSAGYAIYSVFADAVWAVVFAIIWGLIIFNF
                     DRFLVSTMRKYGVSRRKQIWMAMPRLALALLIGFTIARPLELKIFEKEIDVQMAANRH
                     QKMLMNDSLLQAENRVTLQSAQLERDRLGARKASLEDTLHQLQQDYIREADGTGGSQR
                     RGIDKLTRLKQQAYETAARQYAPELLQLERQIHYQDSLIATANAGRELRNRQYEAQVV
                     ADVGFLERNKALSDLSAHESSVFWTSLLLSLLIILIETGPVLSKLIMNTGPYDLALAS
                     LELRQMASAEDELRRDKELRQDKRKEIDGKQKEMTNGLLQQLSDLQKKHVKEELDQWE
                     RGEDTIPKRVPLEEVTRRIRERYGFRGEDVL"
     gene            3544..3792
                     /locus_tag="VKQ52_11420"
     CDS             3544..3792
                     /locus_tag="VKQ52_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87847.1"
                     /translation="MENLKTELVRIEGIDGAGDFVLEEKDHGDLVVYDIYREDRYLMT
                     LARDGGILFMNFDADERDKELFKLSHLNQFIEKIQAIF"
     gene            complement(3859..4083)
                     /locus_tag="VKQ52_11425"
     CDS             complement(3859..4083)
                     /locus_tag="VKQ52_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87848.1"
                     /translation="MKINSINAWPTTLPALPESTIATYGTKTRRNRPGVAKPFAPPAP
                     PDEETIRRLNALAAKNPFCTPYKAKKHELL"
     gene            4245..6182
                     /locus_tag="VKQ52_11430"
     CDS             4245..6182
                     /locus_tag="VKQ52_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87849.1"
                     /translation="MLIRLAAAIALSWCCLVSVGQDSAGHRSLPDTFPRSSPADTIPR
                     GQVRDSTRRRFDRWRNNNIFQFFRNAITKGRPDSVVYINTLAPLNTKSESPFKPFEGK
                     IIRHIYIRGYGFEQTFTDTSRRLEYFGTNLLNHLHRKTRDWVIRDALFIKENTPVNAF
                     KLADNERLIRSLNYIQDARILVTPQPDNSDSVDLVVVVKDLFSIAGALGSLGYPPLSI
                     RGNISEANFLGMGQRIQLGANLEQNRSPAFGPQFLYSKTNISHSFVNATASYTQINSN
                     IYDGTPDETAWFIQLDRPLYAPYAHLAGGFRVGDFQNFNVYAKPDSLFYKYHYNTRDG
                     WIGWNIGSNRFLSNTSVRDRRFVAFRYFKNDFDSVPGKIGNTFNFRFNDREAALTSFT
                     FFRQDFYKTNYVYGFGTTEDLPIGYNVAFTGGWYRQLNLNRLYTGVDANAYVVTKRGG
                     FAQLFLRSGVFLYRGQGQDGSVLIGASYYSPLFVYPNVKIRQYINFSFTRQINRLGID
                     PLTINNVFGLRYFTGDSTFGNMRITLHSETTFFLNYKFLGFKFAPFAFGDLSFLTPED
                     DRLQKSALYHGIGAGIRTRNENLVFNTIEFRMVYFPRKTMQNNSFKIMVNTGIQFRYN
                     STYVRQPDVVFLNTDGLNSIY"
     gene            complement(6212..6829)
                     /locus_tag="VKQ52_11435"
     CDS             complement(6212..6829)
                     /locus_tag="VKQ52_11435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLZ87850.1"
                     /translation="MHTILFLGDSYTFGEGVPLHQGYPYQTVQLLRAAGQDIAAPEII
                     ARTGWTTDELAHTLSFVRLLPHYDFVTLLIGVNDQYRGYPTGEYAPSFGRLLQRAIQL
                     AGTPSKTFVLSIPDWGYSPFAATRDKTLISGEIDAFNDLAKDIAQRQKVHVIDITAHS
                     RTTGAGPESFTADGLHPAASTYHFWAQQLAGAISATLPSRELPID"
     gene            complement(6944..>8349)
                     /locus_tag="VKQ52_11440"
     CDS             complement(6944..>8349)
                     /locus_tag="VKQ52_11440"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HLZ87851.1"
                     /translation="LNLIASNIYNIQGGPLSSIYKQQQVGNGNNKYFTAQTDYTLPLG
                     EKSKFEAGARAAIRNNSSVNDIDTLGADNAFHVAGLLSSQYTFRDRVFAGYVTYGNAI
                     RDFTYQLGLRGESSDYNGTQNYTNFNVAGHQQDTVGHFSNSFPLSLFPSVFLTQKLGG
                     NQDLSLNYTRRIDRPNFFQLFPFTDYSDTLNVSRGNPGLKPQFTNSFELAYEKTFPGN
                     NTFLASADYKRTNDLITRNPEAGINPISGQPVIISTFINAKSSFVGGFELIGRNAITK
                     WWDLTSNINIFTSKINTIDTGLFAVTPVGQTWSWYGKINNSFKLTKALTLQLSGDYTS
                     KTVLPPGGSASNGGGGGGGRGFGGQVSGNANGYSRPTGGVDAALRYEFLKNKAAAITL
                     SVSDVLRTRVNSVYTYAATFEQDAVRRRDPQFFRLQFNYRFGKFDMTLFKRKNLKGEQ
                     NNIEGMQGGGGGGPQSN"
BASE COUNT         1671 a         2064 c         2699 g         1915 t
ORIGIN      
        1 tggtcgccgg cggtgtggga attgcgggca tgcagacggg gatctatacg ctgaacagtc
       61 cgggtggctg gcagatcatc ggccggacgc cggtgaggct tttcgatccg caggcagatc
      121 cgccgatccg gttgcaacct ggggacaggg tccggttcta tccggttacg cccaccgagt
      181 tcagggagct gtccgggcgt cccggatagt aagcctttta aataatcggt gatggttatg
      241 gtttgttaat gtagtagctt agaatacccc gagtgcaatt ttgcaaggta atcccgcctg
      301 atgaagccat cgttgcgacc cccaccaaat caaaatcaaa ctatggcttt ctccattatt
      361 aaacccggct tgctcgatac tatacaggac ctgggcaggc atggtttcgg caactggggc
      421 gtcagtcccg gcggagcgat ggacagttat gcggcgcagg tggcgaatct gctggtcggc
      481 aatgacaagc aggaagctgt gatggaaatt catttccccg gtccgcagat cctcttcgaa
      541 cagaacacgc ttatcgcgat cacgggcgct gatttttccc cgacggtgaa tgacgaggtg
      601 atcccgcgct ggcagcctgt cgtggtaagg aagaacaccg ttcttcattt tccttcgctg
      661 gtgcagggcg gaaggtgtta cctgagcgta catgggggtt attgtctgaa gaaatggctc
      721 aacagctaca gtacgaacct gaaggccggg gccggtggct ggcatgggct ggccctgaag
      781 aagggggatg agctcccatt caatgagaat accatctatt ttgcgggatt attaaaggac
      841 gaaagcaatt atgaggtgtt gccctggcgg gtgaatccgg acaagatcta ccagtacccg
      901 catgagatcg ggtttatccc gggacatgag tgggggttgt tgtcggctgc ctcgaagagt
      961 gttttcctcg agaataattt cctgatccat ccttcttccg accggatggg ttaccagttg
     1021 aagggggcgc cgcttgccct cgatcagccg gtggagctgg tgtcttccgc cgtcaatttc
     1081 gggacggtgc agttgttgcc caatggtcag cttatcgtgc tgatggccga tcaccagacg
     1141 acgggcggct atcctcgcat tgcgcatgtt gtctctgccc atctgcccaa actggcgcag
     1201 ctgcggcctt ccgatacgat acaattcaaa ttgatggaac tcgaaaccgc cgagcggatg
     1261 gttatttccc gccagaagga gctgcatatc ctgcagcgca gttgcatgga tcacttaaat
     1321 gaactggtgt gccagtagat ctgaactgcg acatggggga ggggatggat acggacgcgg
     1381 ctattttccc ttttatcacc agtacgaata ttgcctgtgg cgcccatgcg ggtgacgggg
     1441 acaccatgcg caggacggtg gagctggcga tggtgcaggg ggtggcgatc ggtgcgcatc
     1501 cttcgtaccc ggaccgtgcg cggttcgggc gggtggatat ccttggggcg ggtttgtcgc
     1561 cggctgcgct caaggattct ttggccgcgc tggaggattc cttgtttgag caactgcgaa
     1621 gcctggatgc cgtatgccgg gaactgggcg tccggatgca tcatgtgaag ccgcatggag
     1681 ccctttataa ccgggcagcg cgcgatgtcc tggtgagcgg gctgatctgc cgggtggtag
     1741 cggccgttga tgcttcgctg ttgctttacg ggcttagcgg gagtggaatg tcggctgcgg
     1801 cagcggaggc aggcctcggg tttgtcagcg aggtctttgc ggaccggggt tatcgtcccg
     1861 acggcagcct ggtgccgcgg tcggaaccgg gggccctgat cgaggatgaa gctgctgccg
     1921 tggcgcaggt gctgaagatg gtgcgcgaag gccgcgtggc ggtcaccggc ggcggggagg
     1981 tgacgctgcg cgcggaaacg gtctgtctgc atggggatgg ggcgcatgcg gtggagtttg
     2041 cgcggttgat ccatggggca ctggccgcgg agggggtgaa aatggtggct cctgcctgaa
     2101 aacggaggct cctgcctttt agcatttcct ttgcaatttg tcgggagaat tcccgttctt
     2161 tgtttgttcc cactccaaca caatgaagac ccgcgcgcgg gttaacccca atctattttt
     2221 ttatggcaac aacgacggat caaagccggc tggaaggcgg ctatacctat gattcattca
     2281 caggggtgag cagccgggca aagcggccgg tggaaaccct ttttttgtgg tggtgcagcg
     2341 gggcgtacca gggattactg aaggactgtc cttcggagca gaccaagtat gccgggctcg
     2401 gcggggtgtt gctggcgacc tttgtgctgg cggcgctctc ggcgggctat gctatttaca
     2461 gtgtttttgc cgatgcggtg tgggcggtgg tgtttgcgat catctggggg ctgatcattt
     2521 tcaatttcga ccgctttctc gtttcgacga tgcgcaaata tggcgtgtcc cggcggaagc
     2581 agatctggat ggcgatgccg cggctggcgc tggcgctgct gatcgggttc acgatcgcgc
     2641 ggccgctgga gctgaagatc ttcgagaagg agatcgatgt tcagatggcg gccaaccggc
     2701 accagaagat gctgatgaat gacagcctgt tacaggcaga gaaccgggtg accctgcagt
     2761 cggcgcagct ggagcgggac cggcttggcg cgcggaaggc ctccctcgag gacacgcttc
     2821 accagctgca gcaggattat atccgggagg cggatggtac gggcggctcg cagcgccggg
     2881 gcatcgacaa actcacccgg ctgaaacagc aggcttatga aacggctgca cgccaatatg
     2941 cacccgaatt gctgcagctg gagcggcaga tccattacca ggacagcctg atcgcgacgg
     3001 cgaatgcggg ccgcgagctc cgcaaccgcc aatatgaggc gcaggtggtg gccgatgtcg
     3061 gtttcctgga gcggaacaag gccttgtcgg acctctcggc gcatgagtcg agtgttttct
     3121 ggaccagcct gctgttgtcg ctgttgatca tcctgatcga gacgggaccg gtgctgtcca
     3181 agctgatcat gaataccggt ccatacgatc ttgccctcgc ctcgctggag ctgcggcaga
     3241 tggcgtcggc ggaagacgag cttcggcggg acaaggagtt gcggcaggac aaacggaagg
     3301 agattgacgg gaagcagaag gagatgacga acgggctgtt gcagcagctt tcggatctgc
     3361 agaagaagca tgtcaaagaa gaattggacc agtgggagcg gggggaggat acgatcccga
     3421 agcgggtgcc gctggaggag gtgacgcgca ggatccggga gagatacggg ttccgggggg
     3481 aggatgtttt atagggtgtt ggtgtgatgg cccgggtttt tcggtatatc cccaaaaggc
     3541 gatatggaaa atctaaagac cgagcttgta cgaatcgagg gtatcgatgg ggccggggat
     3601 ttcgtgctgg aggaaaagga tcacggcgat ctcgtcgtct atgatatcta tcgtgaggat
     3661 cgttacctca tgacgctggc cagggacggc ggcatcctgt tcatgaactt cgacgcggac
     3721 gagcgggaca aggagctttt caagctatcc catctgaacc agttcatcga gaagatacag
     3781 gctattttct aatgcaaatg gaggtctccg tttgtagaaa ttccctccca ggaaggaggg
     3841 aatttttttc ccagaaattt acaggagttc gtgttttttc gccttatacg gcgtgcagaa
     3901 aggatttttt gccgcgagcg cgttgaggcg gcggatcgtc tcctcatcgg gcggggcagg
     3961 tggtgcaaac ggttttgcga cgcctggtct gtttctgcgt gttttggtgc cgtaggtggc
     4021 gatggtcgat tcgggtaagg cgggtaaggt cgtgggccag gcgttaatag aatttatctt
     4081 catgggaatg tattgtgggc tagtatattc aaaaaggata ccatgcaatt tgtattggca
     4141 ttttataaca ttatgctaac caaaaagaag gcagcgatat tgacaagtta ttattcttat
     4201 attgggccat tgataatcaa tggtcacatt ttttgaggaa aggaatgctg atacgacttg
     4261 cagcggctat cgcccttagt tggtgttgtc ttgtttctgt cgggcaggac agcgccggcc
     4321 atcgatccct gcccgatacc ttcccgcgct cgtccccggc cgacaccatt ccgcgcggcc
     4381 aggtgcggga cagcacccgc cggcggttcg accggtggcg gaataacaat atcttccagt
     4441 tctttcgcaa tgccatcacc aagggaaggc cggattcggt ggtctacatc aatacccttg
     4501 cgccattgaa tacaaagagc gaaagccctt tcaagccctt tgagggcaag atcatccggc
     4561 atatctatat ccgggggtat ggtttcgagc agacctttac ggatacgtcc aggcggctgg
     4621 aatattttgg tacgaacctg ctgaaccacc tgcaccgcaa gacgcgggac tgggtgatcc
     4681 gggacgcgct ttttatcaag gagaatacgc cggtgaatgc gtttaagtta gccgataacg
     4741 aacggttgat ccgcagcctc aattatatcc aggatgcacg gatcctggtg accccgcagc
     4801 cggacaactc cgactcggtt gacctcgttg tagtggtaaa ggacctgttc agtatcgcgg
     4861 gcgccctggg tagcctgggc tatccgccgc tcagcatccg ggggaatatc agcgaggcca
     4921 atttcctggg gatgggccag cggatccagc tgggcgccaa tctggagcag aaccggtcgc
     4981 cggctttcgg gccgcagttc ctgtacagca agacgaatat cagtcattcc tttgtcaatg
     5041 ccaccgcctc gtatacccag atcaattcca atatctatga tgggaccccg gatgagacgg
     5101 cctggttcat acagctggac cggcccctgt acgcgcctta tgcccatctg gcgggtgggt
     5161 tccgggtagg ggattttcag aatttcaatg tctatgccaa gccggacagc ctgttttaca
     5221 agtatcatta taacacacgc gacgggtgga tcggctggaa catcggctcc aaccggtttc
     5281 tcagcaacac ctcggtgcgt gaccggaggt tcgtcgcgtt ccgctatttc aagaacgatt
     5341 ttgacagtgt gcccggcaag atcggcaaca cgttcaattt ccgcttcaac gaccgggaag
     5401 ccgcccttac ctcttttacc tttttccgcc aggacttcta caaaaccaac tatgtctatg
     5461 gcttcgggac aacggaggac ctgcccatcg ggtacaatgt agccttcacc ggcgggtggt
     5521 accggcaatt gaacctgaac cggctttata cgggggtcga tgccaatgcc tatgtcgtta
     5581 cgaaacgggg cggatttgcc cagctgtttc tccggagcgg ggtattcctc taccggggcc
     5641 agggccagga cggcagtgtg cttatagggg cgagttatta cagcccgctg ttcgtgtatc
     5701 cgaatgtgaa gatcaggcaa tatatcaatt tcagttttac ccggcagatc aaccggctgg
     5761 ggatcgatcc gctgaccatc aacaatgtat tcgggttacg ctatttcacc ggggattcga
     5821 cctttggaaa tatgcggatc acgcttcatt cggaaacaac cttctttctc aactataaat
     5881 tcctcggctt caagtttgcc ccctttgctt tcggggatct ttcctttctc acgcccgagg
     5941 acgacaggct gcagaaatcc gccctgtacc atggcatcgg tgcgggtatc cggacgcgta
     6001 atgagaacct cgtgttcaac acgatcgagt tccggatggt atatttcccg cgcaagacga
     6061 tgcagaacaa ttcctttaag atcatggtca atacgggtat ccaattccgg tataacagca
     6121 cctatgtgcg acagccggac gtcgtctttc tcaacaccga tgggttgaac agtatttatt
     6181 gatggcggcg gccgacgccg gtgggagggt gctagtctat cgggagttcg cggctgggga
     6241 gggttgcgga aatggcgccg gccagttgct gcgcccagaa gtggtaggtc gatgcggcgg
     6301 gatggaggcc gtcggcggtg aagctttccg ggccagcgcc ggtggtacgg ctgtgggccg
     6361 tgatatcgat gacatgtact ttttgccgtt gggcaatatc ttttgcgagg tcgttaaacg
     6421 cgtcgatctc cccggagata agggtcttgt cgcgggtggc ggcgaaggga gagtagcccc
     6481 agtcggggat ggagaggacg aaggtctttg acggcgttcc ggccagttgt atcgctcttt
     6541 gcaggaggcg gccaaaggag ggggcgtatt ccccggtggg gtacccgcgg tactggtcgt
     6601 tgacgccgat gagcagggtg acgaagtcgt agtggggaag gaggcggacg aaggaaaggg
     6661 tatgcgccag ttcgtccgtc gtccagccgg tgcgggcgat gatctcgggg gcggcaatgt
     6721 cctggccggc ggcgcggagg agttgtacgg tttgataggg gtacccctgg tgcaggggga
     6781 cgccttcgcc aaaggtgtag gagtcgccga gaaagaggat ggtgtgcatg gctgtaagat
     6841 aaagaaaaag aggccgtctc aaaccaaaag tagacgggcc tctttttaca ttatgtccgg
     6901 taagcaagct taccggggga gcgccggcgg gcgccctttt ttgctagttc gattgtgggc
     6961 cgccgccacc gccgccctgc atgccttcta tattgttttg ttctcccttg agattcttgc
     7021 gcttgaaaag cgtcatatcg aatttcccaa agcggtagtt gaattgcaga cggaagaatt
     7081 gcgggtcgcg ccggcgtacc gcatcctgtt cgaaggtggc cgcataggta tatacgctgt
     7141 tgacgcgggt gcggaggaca tcgctcacgc tcagggtgat ggcggccgcc ttgttcttca
     7201 ggaactcgta tcgcagggcc gcgtcgacgc cgccggtggg ccgggaatag ccgttcgcgt
     7261 tgccgctgac ctggccgccg aagcctctgc caccaccgcc gccgccaccg ttgctggcgc
     7321 tgccgcccgg cgggagcaca gttttggagg tatagtcgcc ggaaagctgg agggttagcg
     7381 cttttgtcag tttaaagctg ttgttgatct tcccatacca gctccaggtc tggcccaccg
     7441 gggttacggc aaagaggccg gtgtcgatgg tgttgatctt cgaggtgaag atgttgatat
     7501 tgctggtgag atcccaccat ttggtgatgg cgttgcggcc gatgagctcg aagccgccga
     7561 cgaagctgga ctttgcgttg ataaaggtgc tgatgatcac gggctggccc gaaatggggt
     7621 tgatgccggc ttcggggttg cgggtgatca gatcgttggt gcgcttgtaa tcggccgagg
     7681 caaggaacgt attgttgccc gggaaggtct tttcataggc cagttcgaag gaattggtga
     7741 actggggttt cagtccgggg ttcccgcggc tcacgttgag cgtgtcggaa tagtcggtga
     7801 aggggaacaa ctggaagaaa ttgggccggt cgatccgccg cgtatagttc aacgagaggt
     7861 cctggttacc gcccagtttc tgggtcagga agacgctggg gaacaggctt agcgggaagc
     7921 tgttggagaa gtgaccgacg gtatcctgct ggtgtcccgc gacgttgaaa ttggtgtagt
     7981 tctgggtgcc gttgtaatcg gaactttcac cgcgcagtcc caactggtag gtgaaatccc
     8041 tgatcgcgtt gccataggtg acatagccgg caaagacgcg gtcgcggaag gtgtactgcg
     8101 aggagagaag acccgccaca tggaaggcat tatcggcgcc cagggtatcg atatcgttga
     8161 cgctgctgtt gttgcggata gcggcgcgcg ccccggcctc gaacttggat ttttcgccca
     8221 acgggagggt ataatcggtc tgcgcggtga aatacttgtt gttgccattg cccacctgtt
     8281 gctgtttgta gatggatgac agcgggccgc cctggatatt gtagatgttg gaggcgatga
     8341 ggttgaggt
//
LOCUS       DATGFG010000613         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1495386, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000613 DATGFG010000000
VERSION     DATGFG010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495386"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VKQ52_11445"
     CDS             complement(<1..309)
                     /locus_tag="VKQ52_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87852.1"
                     /translation="MKKIYVLLQNGYLSLASLLAFFLLPGLSGFAQSCPMNGVTSLNA
                     YPNTYYPGTQALVSPGNKSFTVGAVTYGTTPISAGDILLIIQMQGAQISSGNNSNYGD
                     G"
     gene            complement(485..1195)
                     /locus_tag="VKQ52_11450"
     CDS             complement(485..1195)
                     /locus_tag="VKQ52_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87853.1"
                     /translation="MKVRGFLVFGLLVLSGSVFSQARRDSSFSLLVNPSISFTHANDV
                     HINHWLAKYGYPTVPSVPSSYNFEIAAIPASSSLLYCIRLSSINSVNNLSSFNIMAGL
                     YTAVVKKHSFLLFLGGATGFHGDIITLNGKLPAEYQELASRIHGPLALRRTGLCLEPG
                     ARLFWYPIQLGIVQIGAQASLAYDWDVNSHWRLGYYSNNHGKYSHFKGIARPSDQQKV
                     SEHGLSYGAGLSFRVNLH"
     gene            complement(1248..>2850)
                     /locus_tag="VKQ52_11455"
     CDS             complement(1248..>2850)
                     /locus_tag="VKQ52_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HLZ87854.1"
                     /translation="GLVKNRDRHEKDDRPGRNDASRFVMGWNNEKKREALQHRRSQLL
                     DDAEQAAEALQRATHRNRRLQDQVFAVKHLREHADFRSINVAALQQAIHHIEEQTGKL
                     KKGSDQLKTLTQQLEDIRQQIEARQKERDQLVEKASLYEHDLQAREKDRQGLSAFVQH
                     LTEADKDLLLQFQQKHGALLAENPLTPDNIDDWYQQIMAAAEQTARQYEDSAAKEGRD
                     LDRAIHKIKNPPLEILQRFTDWSADTQQLPVEREHAGEYLEWLEKLESENLPKYKKDF
                     ERLLHDTAVIKMGVLNEELESWERKIRNSITTLNQSLAGINFNRLPDTYIQLGIRPVP
                     DTVIKEFRSRLLNALPQAADWQQNSFEEKALHFKQNVQSFIDTLDESEPYRNRVLDVR
                     NWFEFWADEKFRETHELKKTYRQMGQLSGGEKAQLTYTILCSAIAYQFGITREGRNSR
                     SLRFIAVDESFSNQDEEKATYLMELCKQLHLQLLVVTPSDKIQIVENFIAHVHLVQRV
                     NNRHSILFNMTRKELKQKRALVVGE"
BASE COUNT          564 a          703 c          867 g          716 t
ORIGIN      
        1 gccatcgcca taattgctgt tattgccgga gctgatctgc gcaccctgca tttggatgat
       61 caggaggata tcaccggcgc tgatcggggt ggtgccgtag gtgaccgcac caacggtgaa
      121 ggacttgtta ccgggcgaga cgagggcctg tgtgcccgga taatatgtat tgggataggc
      181 gtttaagctg gtgacgccgt tcatgggaca actttgtgcg aaaccggaga ggcccgggag
      241 gaggaagaag gccaggaggc tggcgaggga gagatacccg ttttgcaaca gcacgtagat
      301 ctttttcatc ggtaacagat tttgagatta ggggatagta gggtattggc tctgtagtag
      361 tatggccaat tagataccaa ttccgacgga attaagaacc aatcaaatag ccgaatttaa
      421 taatgtgagc atatcccgct gcgggagaaa aattcccttg ctgggaagaa ccaggggttt
      481 ctgcttagtg gagattaacg cgaaagctca ggccggcgcc ataggagagg ccgtgttcgc
      541 ttaccttttg ctggtcgctg ggccgggcga tgcctttaaa atgactgtat ttgccgtgat
      601 tgttgctgta atagccaagc ctccaatgag agttcacatc ccaatcgtag gccaggctgg
      661 cctgggcgcc gatctgtacg atacccagct gaatggggta ccagaagagc cgggcgccgg
      721 gttcgagaca gagacccgtc cggcgaaggg ccagggggcc gtggatccgg gaggcgagct
      781 cctggtattc cgccggcagt ttaccgttca gtgtgatgat atcgccgtga aagcccgtcg
      841 cgccgccaag gaagaggagg aaggagtgtt tctttaccac ggcggtgtac aacccggcca
      901 tgatattgaa ggagctcagg ttattgacgg aattgatgct cgagagccgg atgcagtata
      961 gaagggagga ggaggccggg atggcggcta tctcgaagtt atagctgctg ggaacggagg
     1021 gtacggtggg atagccatat ttcgccagcc agtgattgat atgcacgtcg ttagcgtggg
     1081 taaaactgat gctggggttg acgagcaacg aaaagctgga gtccctccgg gcttgtgaaa
     1141 ataccgaccc gctaagaacc aacaaaccga aaaccagaaa cccgcgaacc ttcatctcat
     1201 aaaagtagaa aaaatcgggg gcagtgcata gtcgattaac aaaaatctca ttcgcccacg
     1261 accagcgctc ttttctgctt tagctctttt cgcgtcatat taaaaaggat gctatgccgg
     1321 ttatttaccc gttggaccag gtgaacatgg gcgataaagt tctcgactat ctggattttg
     1381 tcactcgggg tcactaccag caactgcagg tgcagctgtt tacagagttc catcaggtag
     1441 gtggcttttt cctcgtcctg gttgctaaaa ctctcgtcga cagcgatgaa acgcaggctc
     1501 cgggagttgc ggccttcccg ggtgatgccg aactggtaag cgatagcgct gcaaaggatg
     1561 gtataggtga gctgcgcctt ttcgccgccg gataactgcc ccatctggcg gtaggtcttt
     1621 tttagttcgt gcgtctcgcg gaatttctca tcggcccaga attcgaacca gttacggacg
     1681 tccagcaccc ggtttcgata gggctcgctt tcgtcgaggg tgtcgataaa ggactggacg
     1741 ttctgtttga aatggagcgc tttttcctcg aaactgttct gctgccagtc ggcggcctgg
     1801 gggagggcgt ttagcaggcg gctccggaat tcctttatca cggtatccgg aacggggcgg
     1861 atgcccagtt ggatataggt atccggcagc cggttgaagt tgatcccggc cagggactgg
     1921 ttcaaggtgg tgatgctgtt cctgatcttt ctttcccagc tttccagctc ttcgttcagg
     1981 acgcccattt tgatgacggc tgtatcgtgg agcagccgct cgaagtcttt cttgtatttc
     2041 ggaaggttct ccgactccag tttctccagc cattcgagat actcgcccgc gtgttccctt
     2101 tcgacgggga gctgttgtgt gtcggccgac caatcggtga accgctggag gatctcgagg
     2161 ggcgggttct ttatcttgtg gatggccctg tccagatcgc ggccttcttt tgccgcggaa
     2221 tcctcgtatt gtcgggcggt ttgttccgcc gccgccatga tctgctgata ccagtcgtcg
     2281 atattgtcgg gggtcagcgg gttttcggcc agcagggcgc cgtgtttctg ctggaattgc
     2341 aaaagcagat ccttatcggc ttccgtcagg tgttggacga aggcggagag cccctgccga
     2401 tctttttccc gggcctgaag gtcgtgttcg taaagggaag ctttctcgac cagctggtcg
     2461 cgctcttttt gtcgggcttc gatctgttgc cggatatctt ccaattgctg tgtcagggtc
     2521 tttaactgat cgcttccttt ctttagtttg cccgtctgct cttcgatatg gtggattgcc
     2581 tgctggagcg cggcgacgtt gatggatcgg aaatcggcgt gttcgcgcag gtgttttacg
     2641 gcgaagacct ggtcctgcaa cctgcggttg cggtgggtgg cccgctgcag cgcttccgcg
     2701 gcctgttccg catcgtcgag cagttgcgac cggcgatgtt gcagggcctc ccgtttcttt
     2761 tcattgttcc agcccatgac aaagcggctg gcgtcgttgc gtcccgggcg gtcgtccttt
     2821 tcatgccgat ccctgttctt taccaaaccc
//
LOCUS       DATGFG010000614         7179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1193949, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000614 DATGFG010000000
VERSION     DATGFG010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193949"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VKQ52_11460"
     CDS             complement(<1..1514)
                     /locus_tag="VKQ52_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ87855.1"
                     /translation="MTIQRLLTKMVSFTLLCVLFTHSAFSQTKTVTGKVLDDKGAPVQ
                     GATVTAKGSRVGTSTGADGSFTLSVSSNVTTLVISSVGFSTQEVAVSDGPLSVALAPN
                     QASLNEVVVIGYGTTVRKDVTGSVATVRAKDFNKGVTTPEELLIGKVAGLQVANNSGQ
                     PGGTTITKIRGNNSIVSGNNPLYVVDGVPLDATSPIPGQKAASVGTSPLNNPLLFLNP
                     GDILQIDVLKDASSAAIYGSRGENGVVLITTNKGNGKPAVDGSVTYTFPTGLMKSVDV
                     LSAAQYRSELNVYGFHSDSGLSLNPFQQIVRHTPSALYSAAISGGNENGKYRASFAAN
                     DNQGIILKSALQQYTATFSGEHYAINKRLRVNVNLTASHYTLQSAPISTDAGSTGNLI
                     SAAMNWNPTLRLVNSDGSYMQTNPSGQINPLALSTYSNDYSHVTQILGNAGLSFQILP
                     FLSYNFLYGINYGSATRDAELQGLIAATGSNADGKGSAAFASGSLFSETITHTLT"
     gene            complement(1704..2438)
                     /locus_tag="VKQ52_11465"
     CDS             complement(1704..2438)
                     /locus_tag="VKQ52_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HLZ87856.1"
                     /translation="MGLQETSVIFQKIRYRHYFLCHYLPLTSGIDTVSRSLLKFKRGA
                     QPDLENWLHRTRCALRETPISLPPDTIIIRALHHNETSPSQNRPISLDLLGQTLSGHF
                     HFPYLPGLLAKTRPTNTNQALTLRQRQAELQDVWRINPAALLNIANPISNPSGATDPS
                     APINPAALTASTADNASTPDNTPTPNNPSTPGNTSTPGAHPPLTSILLIDDILTTGAT
                     ARTIIQTLLQAFPTCTITIFTLAKVE"
     gene            2498..3238
                     /locus_tag="VKQ52_11470"
     CDS             2498..3238
                     /locus_tag="VKQ52_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLZ87857.1"
                     /translation="MNNTNRMNTGMKFLADWEARFAAYIREHGELTDGSHDLGHFQRV
                     WKAARFINREEGGMADELILLAAAYFHDWVSLPKNHPRRSESSRLSAEKAVELLSGVV
                     SGFPVEKIEGVRHAIHAHSFSAGVEAKTAEAKILQDADRMEALGAIGAARTFYIAGQL
                     HTQLFDADDPLAASRPLDDRRFALDHFEVKLLKLPALMNTAAGRRLAEKNAAWLRLFA
                     QKVKDEIEGRYGDGDGEVGGYAEGVIEG"
     gene            complement(3327..4562)
                     /locus_tag="VKQ52_11475"
     CDS             complement(3327..4562)
                     /locus_tag="VKQ52_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLZ87858.1"
                     /translation="MSYLLPRHWCLLLLLPLATRAAAGPAKRTAVPTNKITGPTNNIA
                     GPSDTTIQIVFTSDIHYGITRPAFDGDSNVASYLVNRRMIATINTLPALPLPPGQGVN
                     AGQPVGPIDYIMISGDIANRQEPPYPTAAVSWKQFTRDYGEGLTVKDHHGHPSTFLLV
                     PGNHDLSDAIGYYKKMDPQTDPTSMVGIYNYMLHPASPKTNATWHFPADKISYSREIG
                     GIHFMFITIWPDSANRIWMEKDLAAVNPNTPVVIVAHDPADGDAAHFRNPNGNHDING
                     GDRFECLLEETLKDNPDPAGKTDGKPQNDLIEQRGFVAFLKAHPNIKAYFHGHNNWNQ
                     FYTYTGPDHDIQLKVFRVDSPMKGKFSAHDETKLSFQLISIDPGSRTLTVRECLWNTD
                     PQHPGKPPVWGESLTMSLE"
     gene            4693..5466
                     /locus_tag="VKQ52_11480"
     CDS             4693..5466
                     /locus_tag="VKQ52_11480"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLZ87859.1"
                     /translation="MKQDESLEEWLERVDQDYLAHISLDCVVFGFHAGQLKILLLKMK
                     HEDRRALPGGFVMKEETLEEAAIRTLKERTGLENVFLQQFCVFSDPSRSDPDHRADDL
                     RRIGIDPEKAQWFAQRFISVGFYALVEFSCVVPRPDLLSDTCDWYNLDERGPLILDHD
                     QILEAALHALQLQLNYQPVGYNLLPRKFTLPELQKLYEAILGKKLDRRNFQRRIFSYG
                     ILRSLDEKRTGVAHKAPYLYSFDLRKYQKALKLGLQGGW"
     gene            5619..>7179
                     /locus_tag="VKQ52_11485"
     CDS             5619..>7179
                     /locus_tag="VKQ52_11485"
                     /inference="COORDINATES: protein motif:HMM:NF039936.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87860.1"
                     /translation="MKTFLPVFSLLLFFYSASAQTQVCPLNNNFSFGNLTHWEAYTGN
                     NAGGNGPNAIKQWYDSSTAAPAGTLGATVLYEYNLPSTAGIQILNASSTDLYGNFSTI
                     PTINGYRYTNSILLGSTAITRSSSAGTGGGYVRGVTYRINVPPGPTDEPYTMTYAYAM
                     VLENGTHNSNDQPLFSATLKIGDSVISCASPKYFLPTLNNANPRDAGATLDTALAKSQ
                     GFNLSPRSSPNANPNGQGAGASEHLQDVWYKGWTEVTFDLGPYRGQQVVLTFETDNCV
                     PGGHFAYSYIALRNTCGGLQVSGPSSACIGSTLVYSVPALTGASYQWSVPPGWSIVSG
                     ADSNILKVQVTNSNGTVTAQEQNSCANLTASLPVTTVPPTVAGSVSKNGEVCSGSNST
                     QLTLTGNVGSVLNWLSTTDGVHYTPITTTAPTYQADNLTTSTAYVAVVQNGESCNIDT
                     ATAAKIVVDPQTVGGALSPSSMQFCLGQFKDALLTLTGQTGTPVNWQSSPDGSTWTDF
                     SPADVDSEYEVS"
BASE COUNT         1415 a         1574 c         2521 g         1669 t
ORIGIN      
        1 gtcagggtat gggtgatcgt ttcggagaac agggagccgc ttgcgaaagc tgccgaacct
       61 ttaccatccg cgttgctgcc ggtggcggcg atgagaccct gaagttccgc gtcgcgggtg
      121 gcgcttccgt agttgatccc atacaggaag ttatagctga ggaaggggag tatctggaag
      181 gacaggccgg cgttgcccag gatttgggtg acatggctat agtcgttcga gtaggtggaa
      241 agggcgagcg ggttgatctg gccgctgggg ttggtctgca tgtagctgcc gtcggaattg
      301 acgaggcgaa gcgtcgggtt ccagttcatg gcggcgctga tcaggttgcc cgtgctgccg
      361 gcgtcggtgc tgataggggc cgattggagg gtgtagtggg atgcagtcaa attgacgttg
      421 acccggagtc gtttgttgat agcgtaatgt tcgccgctga aggtggcggt gtattgctgc
      481 aatgcgcttt tgagaataat gccctggtta tcgttggcgg cgaaggaagc gcggtatttc
      541 ccgttttcat tgcctccgct gatggcggcg gaatagaggg cactgggcgt atgccggacg
      601 atctgctgga aggggttcag gctgagaccg gagtcgctgt ggaagccgta gacgttcagt
      661 tcggaccgat attgtgctgc ggagagcacg tcgaccgatt tcatgaggcc tgtgggaaag
      721 gtataggtga cagatccgtc tacagcgggt ttgccgttac ccttgttggt ggtgatgagg
      781 acaacaccgt tctccccgcg ggacccgtag atggctgcag aagaggcgtc cttgaggacg
      841 tcgatctgaa ggatatcacc ggggttgagg aagagcaggg ggttgttgag gggagaggtg
      901 cccacgctgg cagccttttg gccggggatc ggcgaggtcg cgtccagcgg tacaccatct
      961 actacgtaga gggggttgtt gccgctgacg atggagttgt taccgcggat ctttgtgatc
     1021 gtggtaccgc cgggctggcc gctgttgttg gcgacctgga ggccggcgac cttgccgatg
     1081 aggagttctt cgggggtggt aacgcctttg ttaaagtctt ttgccctgac cgtcgctacg
     1141 gaaccggtga cgtccttacg gacggtggta ccgtaaccga tgacgaccac ttcattgagg
     1201 ctggcctggt ttggcgccag ggcaacgctg agggggccat cggagacggc cacttcctgt
     1261 gtggagaagc cgacggagct gatgacgaga gtagtgacat tagaggaaac cgagagagta
     1321 aaactaccat cggcgcctgt ggaggtgccg actcgtgaac ctttggcggt gacggtcgcg
     1381 ccctgaacag gagcaccttt gtcgtccagg accttaccag ttaccgtttt tgtttgggag
     1441 aaagcagagt gtgtgaaaag tacacaaagc agtgtgaagc ttaccatttt agtaagcaat
     1501 cgttgaatgg tcataaagca aaagtttagc aattattgat cgaaggtaaa aaaattaaat
     1561 tttacttaac aaatttattt ttacgatcta aattattggc gcttatcgtc atggacagct
     1621 gattgcgggt ctttgctgca atgggcgggg tagttttttt gctaaaaggt cttagcaggg
     1681 gatgggttcc cccaaacccc gttttactcg acttttgcga gggtgaagat cgtgatggtg
     1741 caggtgggga aggcttggag gagggtttgg atgatggtgc gggcggtggc gccggtggtg
     1801 aggatatcgt cgatgaggag gatggaggta agtggggggt gggcgccggg ggttgaggtg
     1861 ttgccaggag ttgaagggtt attgggagtc ggggtgttgt cgggagttga ggcattgtcg
     1921 gcggttgagg ccgtgagggc tgcggggttg atgggggcgg acgggtcggt ggcgccggat
     1981 ggattggaga tgggattggc gatgttgagg agggctgcgg ggttgatgcg ccagacgtcc
     2041 tggagttcgg cctggcgctg gcggagggtg agggcctggt tggtgttggt gggccgggtc
     2101 ttggcgagta acccgggaag gtagggaaag tgaaaatggc cggaaagggt ttgtcccagg
     2161 aggtcgaggg agatgggccg gttttgggag gggcttgttt cgttgtgatg gagagcgcga
     2221 ataataatgg tgtccggcgg gagggagatg ggggtttcgc ggagggcgca tcgggtgcgg
     2281 tgtagccagt tctcgagatc gggttgggcg ccgcgtttga atttcagcag gctgcgggag
     2341 acggtatcga tcccggaggt gagggggagg taatggcaaa ggaagtagtg ccggtaacgg
     2401 atcttttgaa aaatgacgga tgtttcctgc agccccatga tctttgcttc aatttttgcc
     2461 ggcggaccgt ggtccgcgcc ataaaaatac gaatcgaatg aataatacga atcgaatgaa
     2521 tacagggatg aagtttttag ccgattggga ggcccggttt gcggcctata tccgggagca
     2581 tggggagctg acggatgggt cgcatgatct cgggcatttt cagcgggtat ggaaggcggc
     2641 gcggttcatc aaccgcgagg agggcgggat ggccgatgag ctgatcctgc tggcggccgc
     2701 ctattttcat gactgggtat cgctgcccaa gaaccatccg cggcgcagcg agtcttcgcg
     2761 gctgagtgcg gagaaggcgg tggaactgct gagtggggtt gttagcgggt tccctgtcga
     2821 aaagatcgag ggggtgcggc atgccatcca tgcgcatagt ttttcggcgg gtgtcgaggc
     2881 gaagaccgcg gaggcgaaga tcctgcagga tgcggaccgg atggaggcgc tgggggcgat
     2941 cggggcggcg aggacctttt atatcgccgg gcagctgcat acacagcttt ttgatgccga
     3001 tgatcccctg gcggcgagca ggccgctcga tgaccggcgt tttgccctcg atcatttcga
     3061 ggtgaagctg ttgaagctgc ctgcgctgat gaacacggcg gcggggcggc ggctggcgga
     3121 gaagaatgcg gcctggctgc ggctttttgc gcagaaggtg aaggacgaga tcgagggccg
     3181 ctatggggac ggggatgggg aggtaggggg ctatgctgaa ggggttattg aaggatagag
     3241 ggttggtgtg aggatggggt atcgggagat cggagctttg atgcggatgg ggttattgaa
     3301 ggataggggg gcttatgagg atggggttat tccaacgaca tcgtaaggct ttcgccccag
     3361 acggggggct tgccggggtg ttgtggatcc gtattccaga gacattcccg cacagtgagg
     3421 gttcttgagc ccgggtcgat gctgatcagc tggaaggaga gttttgtctc gtcgtgcgcc
     3481 gagaatttgc ctttcatcgg ggagtcgacg cggaagacct tgagctggat atcgtgatcg
     3541 ggaccagtgt aggtgtagaa ctggttccag ttgttgtggc cgtggaaata ggcctttatg
     3601 ttgggatggg cctttagaaa ggcgacgaag ccgcgttgtt cgatgaggtc gttctgcggt
     3661 ttgccgtcgg tcttgccggc cgggtccggg ttgtctttca gggtctcttc gagcaggcat
     3721 tcgaagcgat cgccaccatt tatatcgtgg ttgccgttgg gattgcggaa atgggcggcg
     3781 tcgccatccg ccggatcgtg ggcgacgatg acgacggggg tgttcgggtt gacggccgcc
     3841 aggtcttttt ccatccagat gcggttggcc gagtccggcc agatggtgat gaacatgaaa
     3901 tgaatgccgc cgatctccct tgaataactg atcttgtccg ccgggaaatg ccaggtggca
     3961 tttgtttttg ggctggcggg gtggagcata taattataaa tgcctaccat gctggtgggg
     4021 tcggtctgcg ggtccatctt cttataatac ccgatggcgt cggagagatc gtgattaccg
     4081 gggactagga gaaaggtgga ggggtggccg tgatggtctt ttaccgtgag cccctctccg
     4141 tagtcccggg tgaactgttt ccaggacacc gcggcagtgg ggtagggcgg ttcctggcga
     4201 ttggcgatat cgccggagat catgatatag tcgatggggc cgacgggttg gccggcgttg
     4261 acgccctggc cggggggaag cggaagggcg ggaagggtat ttatcgtcgc gatcatccgg
     4321 cggttgacga gataggaggc tacgttgctg tcgccgtcga aagcggggcg ggtgataccg
     4381 taatggatat cggatgtgaa aacgatctgg atggttgtgt cggaggggcc ggcgatattg
     4441 tttgtggggc cggtgatctt gtttgtgggg acggcggtgc gctttgcggg cccggcggct
     4501 gcgcgggtag cgagcggcag caggagcagc aggcaccaat ggcggggaag gagataactc
     4561 atgcaggatg tgggtgaacg ttaaaaattg gggcaaaatt accgctactg gtggttgtta
     4621 ccggttatgt ccatgttaat tcattctgta gaaacatggt aaaaacataa tttttagcct
     4681 attttgggat cgatgaagca agatgaaagt ttagaagaat ggctggaacg ggtggaccag
     4741 gattatctcg cccatatatc gctggactgc gtggtttttg gatttcatgc ggggcagttg
     4801 aagatccttt tgttgaagat gaagcatgag gaccggcggg cgttgccggg tggctttgtg
     4861 atgaaggagg agacgctgga agaggcggcc atccggacgt taaaggagcg aacggggttg
     4921 gagaatgtgt tcctgcaaca gttttgtgtg tttagcgatc cgtcgcgaag tgatccggat
     4981 caccgggcgg atgatctgcg gaggatcggt atcgatcccg agaaggcgca gtggtttgcc
     5041 cagcggttta tcagcgtggg tttttatgca ttggtggagt ttagttgtgt ggtgccgagg
     5101 cccgatctgt tgtcggacac ctgcgactgg tataatctcg atgagcgggg cccgttgatc
     5161 ctggaccatg accagatact ggaggcggcg ttgcatgccc tgcaattgca actgaattat
     5221 cagccggtgg gctataacct gctgccgcgc aagttcaccc tgcccgagtt gcagaagctg
     5281 tatgaggcca ttctcgggaa gaaactggac cggcggaatt tccagcgaag gatcttttcc
     5341 tacgggatcc tgcggagcct ggacgagaaa aggacgggag tggcccataa ggccccctat
     5401 ctttatagtt ttgacttgcg caaataccag aaggccctta agcttggctt gcagggggga
     5461 tggtaatcag ccccttatgc aatccggtgg gcggggaagg ctttcctatt gaaaaaataa
     5521 ctgtcggtcg agtaaactct aatgggaaat ccttcgtaat cctatggggg agtattattt
     5581 ttaggggtga ggggaagcca taaacagtgc atttcattat gaagacgttt ttacctgtat
     5641 tcagtctgct actcttcttt tattccgcct ctgcgcagac gcaggtatgt ccgctgaaca
     5701 acaatttttc ctttgggaat ctcacccatt gggaggctta taccgggaac aatgcgggcg
     5761 gcaacgggcc aaacgccatc aagcagtggt atgattcgag cactgccgcg cccgccggca
     5821 cgctgggcgc tacggtcctg tatgagtaca atctaccttc cactgccggg atccagatcc
     5881 tgaatgcgtc ttccaccgac ttgtacggta acttttccac tattccgact attaacgggt
     5941 atcggtatac caattccatt ttgctgggca gtacggccat tacccggagc agtagtgcgg
     6001 gtacgggtgg cggatatgta cggggagtaa cgtacaggat caatgttccg cccgggccta
     6061 cggatgagcc gtatacgatg acctatgcct atgcgatggt gttggagaac ggtacgcaca
     6121 actcgaatga tcagcctttg ttctccgcca ccctgaagat cggggacagt gtgatctcct
     6181 gcgcgtcgcc aaaatatttt ttaccgaccc tgaacaatgc gaaccctcgg gatgcgggcg
     6241 ccacgctgga tacggcgctg gccaagagcc agggttttaa cctgagtccg cggtcctcgc
     6301 ccaatgccaa cccgaacggg cagggcgccg gtgcgtccga acacctgcag gatgtgtggt
     6361 ataagggctg gacggaagtg acctttgatc ttggccctta ccgcgggcag caggtggtgc
     6421 ttacgtttga gaccgataac tgtgtgcccg ggggacattt tgcctattcc tatatcgcgc
     6481 tccgcaatac ctgcggaggg ttgcaggtga gcggcccctc gtcggcctgt atcgggagca
     6541 cgctggtcta ctctgttccc gcgctgacgg gcgcgagcta tcagtggtcg gtgccgccag
     6601 gctggagcat cgtttcaggg gcagacagca atatcctgaa ggtgcaggtg acgaacagta
     6661 acggcactgt taccgcgcag gagcagaaca gttgcgccaa tctaacggcc tcgctgcctg
     6721 tgacgacggt gccgcccacg gttgcgggga gtgtcagtaa gaatggggag gtttgttcgg
     6781 gctccaacag cacccagctc acccttacgg ggaatgtcgg gagtgtcctg aactggctct
     6841 cgacgaccga tggagtgcat tacacgccga tcacgactac ggctcccacc taccaggccg
     6901 ataatttaac gacttccacc gcctatgtag cggtggtgca gaacggtgag tcctgtaata
     6961 tcgatacggc cacggcggcg aagatcgtgg tggacccgca gacggtgggt ggggcgctta
     7021 gtccttcctc catgcagttc tgtcttgggc agtttaagga tgccttgctg acgctgaccg
     7081 gccagacggg tacgccggtg aactggcagt cctcgccgga tggaagtacc tggacggatt
     7141 tctcgccggc tgatgtggac agtgaatacg aggtttcgg
//
LOCUS       DATGFG010000615         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_898, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000615 DATGFG010000000
VERSION     DATGFG010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2334
                     /locus_tag="VKQ52_11490"
     CDS             <1..2334
                     /locus_tag="VKQ52_11490"
                     /inference="COORDINATES: protein motif:HMM:NF039200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HLZ87861.1"
                     /translation="LFFNLTFFSGILPLIAQDRLVDKDNIVWDGASRDAAGSMPCGGG
                     DIGLNVWVENGDLLFYFGQSGSFDENNALLKGGRVRLRLRPNPLEGVVRQELVLHDGS
                     VVVSGRVGAGSGKGAAVKIRVWVDVFRPAVHVDVDGSRPLAADVTYESWRAADRVMEG
                     KANNANSYKWAWRHAPGGQVVTHGDSIRFQDGGVLFYHRNRDQTVFDVTVRQQGLDTV
                     KAFLYNPLEGLTFGGMLTTTGMRPGERVSGRYGDTDFEGWRLVSDRAVRHRELLLVLS
                     TEQVKDVAAWEAGLRKKVADVRVYSGSARQATADWWNAFWRRSFIYIKPDSPDGSPEW
                     QAGRNYQLFRYMLGCNAHGDYPTKFNGGLFTYDPALTDTSLRFTPDFRNWGGGTMTAQ
                     NQRLVYWPMLRSGDLDLMRSQFSFYLRSQRAAEFRSRVYWGHGGACFTEQLENFGLPN
                     NAEYGWDMPADHDKGVEYNAWLEYEWDTVLEFCLMMLEEERYKGEGSSLAVYVPFIES
                     CLTFFNEHYQYLARLRGRKTLDGDGHLILYPGSGGETYKMAYDASSTIAGLHTVLSRL
                     LELPAGYLTDSSRAVWTTMLQRLPPPAFRRFDGHVTISPAKVWERINNKECPQLYPVF
                     PWGVYGIGRPGLDTAINTYKYDTDAVRFRSYVGWKQDNIFAARLGLVRDAAALTVEKL
                     RDGPRRFPAFWGPGFDWVPDHNWGGSGMIGLQEMLLQTDGKKIFLFPAWPREWDVHFK
                     LHAPYSTTVEAVLRKGKVELLKVEPASRQQDIINFLR"
     gene            2331..>2843
                     /locus_tag="VKQ52_11495"
     CDS             2331..>2843
                     /locus_tag="VKQ52_11495"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HLZ87862.1"
                     /translation="MMKQLLFVFLCCMGGGVHAQSPYAVLEGTGIRDVHWTKGFWAER
                     FAVCRDSMLPHLWRIYTDKHISHAFDNFEIAAGLDTGSHAGPPFHDGDFYKLLEGMAA
                     MYAVTQDKRLDSLMDLAIAVIARSQRPDGYIHTPVVIEERKRSGKATAFEDRLNFETY
                     NMGHLMTAACV"
BASE COUNT          552 a          607 c         1017 g          667 t
ORIGIN      
        1 ctttttttta atttaacctt ttttagcggg attttacctt tgatcgccca ggaccggctt
       61 gtggataagg acaatattgt ctgggacggg gccagtcgtg atgccgcggg gtcgatgccc
      121 tgcggaggcg gtgatatcgg gctgaatgtc tgggtggaaa atggcgattt gctgttctat
      181 tttggccaaa gcggcagttt tgacgagaat aatgcattgt tgaagggagg acgtgtgcgg
      241 ttacggttgc ggcccaaccc gcttgagggg gtggtccggc aggaactggt attgcatgat
      301 gggtcagtgg tggtgtcggg gcgtgtgggc gcgggaagcg ggaagggggc ggctgtgaag
      361 atccgggtgt gggtggatgt attccggccg gcggtgcatg tggacgtgga tggaagccgg
      421 ccgttggctg cggatgtgac ctatgagagc tggcgggcgg cggaccgggt gatggaaggc
      481 aaggcgaata atgcgaattc ctataaatgg gcgtggcggc atgcgccggg cgggcaggtg
      541 gtgactcacg gggacagtat ccgctttcag gacggggggg tattgttcta tcatcggaac
      601 agggatcaaa cggtctttga tgtgactgtc cggcagcagg ggttggatac ggtgaaggca
      661 tttttgtata acccgttgga ggggttgacg tttggcggga tgttgacgac gacggggatg
      721 cggccggggg aaagggtcag cgggcggtac ggagatactg actttgaggg atggaggctg
      781 gtgagtgaca gagcggtacg gcatcgggaa ttgttgctgg tgctaagcac ggaacaggta
      841 aaggatgtgg ccgcctggga ggcggggttg cggaagaagg tagcggacgt gcgggtgtat
      901 tcgggatcgg cccggcaggc gacggccgac tggtggaatg ctttttggcg gcgaagcttt
      961 atctatatca aaccggatag ccctgacggc tcgccggaat ggcaggcagg acggaactac
     1021 cagctgttcc gttatatgct ggggtgtaat gcccatggcg attatccgac caagttcaat
     1081 ggcggacttt tcacctacga tcctgcgctc accgatactt ctcttcgatt cacaccggat
     1141 tttcgcaatt ggggcggtgg gacgatgacg gcgcagaacc agcggctggt gtattggccg
     1201 atgttgcgca gcggggatct tgacctgatg cgatcgcagt tttcctttta cctgcgcagt
     1261 cagcgggcgg ccgaatttcg gagccgggtc tattggggac atggcggcgc ctgctttacc
     1321 gagcaactgg agaattttgg gttgccgaac aatgccgagt atggctggga tatgccggcc
     1381 gatcatgata agggtgtgga atataatgcg tggctggaat atgagtggga taccgtactg
     1441 gaattttgcc tgatgatgct ggaggaggag cggtacaagg gagagggttc ttctcttgcg
     1501 gtgtatgttc cctttatcga aagctgtctg accttcttta acgagcatta ccaatacctc
     1561 gcccggttgc gtggccgcaa gacgctggat ggggacgggc atctgatcct ctaccccggt
     1621 tcgggcggag agacatataa gatggcctat gacgcttctt cgacgatcgc ggggttgcat
     1681 acggtgctgt cgcgactgtt ggaattaccg gcaggatatt tgacggacag ttcgcgcgcg
     1741 gtgtggacga cgatgttgca gcggctgccg ccgccggctt ttcgacgttt tgacgggcat
     1801 gtaacgatct cgccggcgaa ggtgtgggag cggatcaata ataaggagtg tccacaactg
     1861 taccccgtgt tcccctgggg ggtctacggg atcggccggc ccgggctgga tacggcgatc
     1921 aacacttata aatatgatac cgatgcggtg cggttccgga gctatgtcgg ttggaagcag
     1981 gacaatatct ttgcggcccg gctggggctc gtcagggatg ccgctgcgct gaccgtggaa
     2041 aagctacggg atggaccgag acggttcccg gcattctggg ggccgggttt tgactgggtg
     2101 ccggatcata actggggagg cagcgggatg atcggattgc aggagatgct tttacagacg
     2161 gacggaaaga agatcttttt gttccccgca tggccgcggg aatgggatgt acattttaaa
     2221 ttgcatgcgc cttactcgac gacggtggag gccgtgttga ggaaggggaa ggtggagctg
     2281 ctgaaagtcg aacctgcttc ccggcagcag gatattataa atttcctacg atgatgaaac
     2341 agttgttgtt tgtttttttg tgttgtatgg gtggcggggt gcatgcgcag agtccgtatg
     2401 cggtgctgga gggtacgggt atccgggatg tgcactggac gaagggtttc tgggccgagc
     2461 gttttgctgt ttgccgggat tcgatgctcc cgcatctctg gcggatctat accgacaagc
     2521 atatcagcca tgcgttcgat aatttcgaga tcgccgcggg actggatacg ggttcccatg
     2581 cgggtccgcc gttccatgac ggggatttct ataagctatt ggaagggatg gcggcgatgt
     2641 atgccgtcac gcaggacaag cgcctggaca gcctgatgga tttggcaatt gccgtgatcg
     2701 ccaggagtca gcggcctgac ggatatattc atacgccggt ggtgatcgag gagcggaagc
     2761 gctcggggaa ggcgacggcc tttgaggacc ggctgaattt tgaaacgtac aatatggggc
     2821 atctgatgac cgcagcctgt gtg
//
LOCUS       DATGFG010000616         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_962511, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000616 DATGFG010000000
VERSION     DATGFG010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962511"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VKQ52_11500"
     CDS             complement(<1..514)
                     /locus_tag="VKQ52_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ87863.1"
                     /translation="MKNRISLIIADDHEIFRDGLALMLSKQEAVTLAGQAGDGQELLR
                     LVDETRPDMVLTDIKMPRLDGIAAAKIILERYPTTKIIALSMYEEEDLIVEMLEAGAK
                     GYLLKNADKREILEAILTVNEGNIFYCKHTTAHLASLIVKSKFDSHKKSAGPLFTDRE
                     REIIRLICRQH"
     gene            complement(556..1296)
                     /locus_tag="VKQ52_11505"
     CDS             complement(556..1296)
                     /locus_tag="VKQ52_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ87864.1"
                     /translation="MEAKVLNITLYVSLIIGIIIVYFFVSIIRYHRRYMKLQREKIFA
                     EITIRENERKRIAGDLHDSLGPLLSAVKLNISSVDVENAADREILEKTGGYLDEIIGS
                     MRRISHDLLPSTLERKGLLEAIREFLLQVKNKQSVNIQLYIVKEINVPKEKEIHIFRM
                     IQEIVHNTIKHAHAQNLQIGFSEEGGHLLCLTKDDGRGFDKEKVLSSSLGLGLRSLES
                     RCEILNGILTLDSMPGGGTNYFIKIPVG"
     gene            complement(1327..1983)
                     /locus_tag="VKQ52_11510"
     CDS             complement(1327..1983)
                     /locus_tag="VKQ52_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87865.1"
                     /translation="MKAFYIFLLSEIILFPLIVGLVRLRRVKKSGYQPFFILILLGAI
                     MELVSGFLIKVLHAHNIVPINIFILIEWLLIALQFHAWGLLRGRKPLFYALLAVPTLV
                     WVTEYLIFGQIFNFAPYFQVLYCFLIVLFSVNTINFMITHDYRNLFGNTTFLICIGFI
                     IYFIYRIVFQWAYQTSLTGATETSTFIIMLFGYVNALTNIIFGIALLRIPPPQKFTLK
                     "
     gene            complement(1990..2556)
                     /locus_tag="VKQ52_11515"
     CDS             complement(1990..2556)
                     /locus_tag="VKQ52_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87866.1"
                     /translation="MNKTPRPMWLLSKALSVVTVFLFLAGVAGCGPQQGDGDHGKPPI
                     NEDTLRNHVIPIGLAIQYTKGFQAALDSNYHLRPKVLDSLRFSHGEEFPSDVFYDLLS
                     QTNPKQGSAKGIRIYFGRGSDGQFKLVMVPVDSLGNDMIDHLVNLKGKAVPGTAHVES
                     LTVSDGQGFEVGQQCPTACDNGGSGLNQ"
BASE COUNT          749 a          828 c          691 g          673 t
ORIGIN      
        1 tatgctgccg gcagataagc cggatgatct cacgctcccg gtccgtgaac aacggacccg
       61 ccgacttctt atgcgagtcg aacttgctct tgacgatcag cgacgccagg tgggccgtcg
      121 tatgcttgca gtagaagata ttgccctcgt taacggtaag aatggcctcc aggatctccc
      181 gcttgtccgc attcttcagc aaatacccct tggctcccgc ctccagcatc tccacgatca
      241 ggtcctcctc ctcatacatc gaaagggcaa tgatcttggt cgtcgggtaa cgctcgagga
      301 tgatcttggc ggccgcgatc ccatccagcc gcggcatctt gatatcggtg agcaccatat
      361 ccggccgggt ctcatccacc agccgcagta attcctgccc atcccccgcc tgcccggcaa
      421 gcgtgacggc ctcctgcttg gacaacataa gggcaagacc atcccgaaag atctcatggt
      481 cgtctgcgat tatcaggctg attctatttt tcatccggat aaaattagga ctttaccacg
      541 ggacaagaca catttttagc ctacaggtat cttaataaaa taattagtgc caccccccgg
      601 catcgaatcc agggtcaata tcccgttcag gatctcacaa cggctttcca ggctccgcaa
      661 acccagccca agactgctgg acaagacctt ctccttgtca aaacccctcc cgtcatcctt
      721 ggtaagacac aacaggtgcc ccccctcctc gctgaagccg atctgcaggt tttgggcatg
      781 cgcatgcttg atggtgttgt gcacgatctc ctggatcatc cggaaaatat ggatctcctt
      841 ttcctttgga acattgatct ccttgacgat atagagctgg atattcacgg attgcttgtt
      901 cttcacctgc aacaggaact cccggatggc ctccagcaaa cccttgcgct caagagtgct
      961 gggcaacagg tcatggctga tcctccgcat gctcccgatg atctcgtcaa gatacccccc
     1021 cgtcttttcg aggatctccc ggtccgcggc attctcgaca tccacagagc tgatgtttag
     1081 cttcaccgcg gacaaaagcg gccccaggct gtcatgaaga tcccccgcga tccgctttcg
     1141 ctcattctcc cggatcgtga tctccgcaaa gatcttctcc cgctgcagct tcatataccg
     1201 ccggtggtac cggatgatgg atacaaagaa ataaacgatg atgatcccta tgatcagaga
     1261 tacgtaaaga gtaatattca ggaccttggc ttccattttg catgaattgt cggaccacgg
     1321 tttgccctac ttcagggtaa atttctgcgg cgggggtatc cttagcaacg caatgccaaa
     1381 aataatattt gtaagggcat ttacgtaacc gaacagcatt attatgaaag tactggtctc
     1441 ggtggcgccg gtcaggctgg tctggtaagc ccattggaat acgatccggt agataaaata
     1501 gatgataaac ccgatgcaga tcagaaaggt ggtattaccg aaaagattgc ggtaatcgtg
     1561 cgtgatcatg aaattgatgg tattgacgct gaatagcacg atcaggaagc agtacagaac
     1621 ctgaaaataa ggggcaaaat tgaagatctg gccgaaaatg agatattccg ttacccagac
     1681 gagagtgggg acggccagca aagcataaaa aagcggcttt cggccccgga gcaaccccca
     1741 ggcatggaac tgcaaggcaa taagaagcca ctcgatcagg ataaaaatat tgatcgggac
     1801 gatattatga gcatgcagga ccttgatgag aaagccactg accaattcca tgatggcccc
     1861 caacaggatg aggataaaaa agggctggta gccgcttttc tttacccggc gcagacgtac
     1921 cagccctacg ataagaggaa aaaggatgat ctcgctgagg aggaatatat aaaatgcctt
     1981 catacggttt tactggttaa gcccgctccc cccgttatca caggcggtcg gacactgctg
     2041 acctacctcg aaaccctgac cgtcactcac cgtcaacgat tccacatgcg ccgtacccgg
     2101 caccgccttg ccctttaaat taacgagatg atcgatcatg tcgttgccaa ggctgtccac
     2161 cggcaccatc accagcttga actgcccgtc ggaaccccgc ccgaaataga tgcggatccc
     2221 cttcgcactc ccctgcttgg ggttggtttg cgacaaaaga tcatagaaca catccgaagg
     2281 gaactcctcg ccgtgactga acctcagcga atcgagcacc ttcggtctca gatgatagtt
     2341 actgtcgaga gcagcctgga agcccttcgt atactgtatc gccagaccga taggaataac
     2401 gtggttccgc aacgtatctt cattgatggg gggcttccca tgatctccat caccctgctg
     2461 cggcccacag ccggcgacac ctgcaagaaa taaaaagaca gtgacgacgc tgagcgcttt
     2521 cgatagaagc cacatcggcc tcggggtctt gttcataaaa agtgttttgc gttgataaat
     2581 tgatgaagag attagtaatt gaaggacaat atactatttc cgcctatagg gcgtatcagg
     2641 tattaccccc tgattaaaat aagtataact acgcatgatt ttcagtaaga ctgcgctagg
     2701 gcgtctgccc cacaggatgg aaatttgtat taaccttgcc cgatcagaat gtggaaataa
     2761 tttgccagcc cctaaacccc ccatctcccc ctgatcacat accaaaaacc attaaacgtt
     2821 cgtgaggtta ccagcagcaa tcccgtcgac aggtcgggat tcgctgttcg gtactgggcc
     2881 cggtctccgg gccattaaaa ccctaactcc cccggccttc aacgctaccc gcccccgatc
     2941 t
//
LOCUS       DATGFG010000617         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1602411, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000617 DATGFG010000000
VERSION     DATGFG010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602411"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="VKQ52_11520"
     CDS             complement(<1..1633)
                     /locus_tag="VKQ52_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLZ87867.1"
                     /translation="MLTGALSLSQLLPESVQRALAITPEEGSTYLDAQHVVILMQENR
                     SFDHALGTLRGVRGYNDPRAIELPNRNKVWLQTNAEGQTYAPFRLDIRNTRATWMNSL
                     PHSWANQVNARNDGKHDKWLDEKRNSIAAYSHMPLTLGYHTREDLSFYYALADAFTVC
                     DQNFCSSLTGTNPNRLYFWTGTVREQQQEGSRALVWNDDMDYGTLKWAAFPERLEDHG
                     ISWKCYQNEMSIDTGLRGEQDPWLSNFQDNPLEFFAQYNIHLHPPHIGELKRQHAQLA
                     AEIADLEKQIAALPAGDAHIDHLQKNLHQQRQDLEKLKARPGFADGRRLDELPARDQA
                     LHRKAFDTNTGDEHYHALEDLNYTDAGVERTMKAPKGDVLYQFRQDVQSGKLPMVSWL
                     SAPENFSDHPSAPWYGAWYVSEVLDILTQNPEVWKKTIFILAYDENDGYFDHVPPFTA
                     PHPHKQGTGKVSAGIDTRVDFITLEQEQERNGFPEKFARECSIGLGFRVPLIIASPWT
                     RGGWVNSQVFDHTSTLQFLEKFLSNKLGRPVTEPNI"
     gene            complement(2043..2327)
                     /locus_tag="VKQ52_11525"
     CDS             complement(2043..2327)
                     /locus_tag="VKQ52_11525"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ87868.1"
                     /translation="MPQMDAKQAEKISKALSDPTRLKILSEIQKKHDWLYCVDLYENI
                     NLAQPSICHHLKQLTETSIILPEKEGRNIKYTMNNAVIDDYINFLEGLKK"
     gene            2403..>2748
                     /locus_tag="VKQ52_11530"
     CDS             2403..>2748
                     /locus_tag="VKQ52_11530"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HLZ87869.1"
                     /translation="MNFHLTQIPERTTRPRTYGLTMVNDKGLSVAEARNLVSGASPYV
                     DLVKLAFGTPLVTAGLHEKIRLFQDAGIPVYFGGLLLEAFLIRNQFNDYLSLLKEYNI
                     TTIEVSDGSIDIP"
BASE COUNT          640 a          849 c          637 g          622 t
ORIGIN      
        1 agatattcgg ctccgtcacg ggccggccca gcttattgga aaggaacttc tctaggaact
       61 ggagcgtcga agtatgatcg aacacctggg agttgaccca cccgccccgc gtccagggcg
      121 acgcgatgat cagcggaacg cgaaagccaa gcccgatcga acactcccgc gcaaatttct
      181 cgggaaaccc attccgctcc tgctcctgtt cgagcgttat aaaatccacc cgtgtatcga
      241 tccccgcgga caccttcccc gtcccctgct tatgcggatg cggtgcggtg aacggcggga
      301 catgatcgaa atatccatca ttttcgtcgt aggcaagaat aaagatcgtc ttcttccaca
      361 cctcggggtt ctgcgtgagg atatccagca cctccgagac ataccaggcc ccgtaccacg
      421 gcgcactggg atggtcggaa aaattctccg gcgccgacag ccaggacacc atcggcagct
      481 tacccgactg tacatcctgg cgaaactggt acagcacatc ccccttcggc gccttcatcg
      541 tccgctccac accggcatcc gtatagttca ggtcttccag cgcatgatag tgctcatcgc
      601 cggtattcgt gtcgaaagcc ttacgatgca gggcctggtc ccgcgccggc aattcatcga
      661 gccggcgccc gtccgcaaaa cccggccgcg ccttcagctt ctccaggtcc tgccgctgct
      721 gatgtaaatt cttctgtaaa tgatcgatat gcgcatcccc cgccggcaac gccgcgatct
      781 gcttctcgag atcggcgatc tccgcggcca gctgcgcgtg ctgccgcttc aactccccga
      841 tatgcggcgg atgcagatgg atattgtact gcgcaaagaa ctcgagcggg ttatcctgga
      901 aattggagag ccaggggtcc tgctcgccac gaagtcccgt atcgatactc atctcgttct
      961 ggtaacattt ccaggaaata ccatggtcct cgagccgctc gggaaatgcc gcccacttca
     1021 gcgtgccata atccatatcg tcattccaca caagcgcccg cgaaccctcc tgctgctgct
     1081 cccggaccgt gcccgtccag aaataaagcc ggttcgggtt ggtgcccgtc agcgaagaac
     1141 aaaagttctg gtcacaaacg gtaaaagcat cggctaaagc atagtaaaag gacaggtcct
     1201 cccgggtatg atagccaagc gtcagcggca tatgcgaata cgcggcgata ctgttccgtt
     1261 tctcatccag ccatttgtcg tgcttcccat cgttgcgggc attcacctgg ttggcccagg
     1321 aatgcggcag cgagttcatc cacgtcgccc gggtatttcg gatatcgagc cgaaacggcg
     1381 catacgtctg cccttcggca ttcgtctgca accacacttt attacggttg ggaagttcga
     1441 tcgcccgggg atcgttataa ccgcgcaccc cgcgaagcgt gccgagcgcg tggtcaaacg
     1501 accggttctc ctgcatcaaa ataacgacat gctgggcgtc aagatacgta ctgccttcct
     1561 cgggggttat cgcgagagca cgctgaacag attcgggcaa cagttgggaa agactgaggg
     1621 cgcccgtcaa caaggccgtt tttttaagaa aatctcttct ggtatcaggc atccgcgaat
     1681 ctagcgagaa tgcccgacgc acaggttacc aaattcttaa aaaatcaccc atttccgcag
     1741 cctccgtccg gctggcacta ggcattaatt aaataagaga ttacacaaag cgctgcaaag
     1801 cccaatacgg taaacaaaaa attccggccg agacgcttgt tcctttcttc cagcagaggg
     1861 gaagaaaagg cttcttcaca atttaaacaa aactggttca tcttcaatac atttaaatgt
     1921 ttcaatttat caattccatt acaaatgtac aaaacattta aatatttcga tcttttttcg
     1981 ggattatttt ttccccagcc ccgccaaaac ccaacagccc cgcccaaccg ccgccggccg
     2041 ccctatttct tcaatccctc caaaaaattg atataatcat cgatcaccgc attgttcatc
     2101 gtgtatttga tattcctccc ctccttctcg ggcaggatga tcgacgtttc cgtaagctgc
     2161 ttgagatgat gacagatgga cggctgcgca agattgatat tctcgtacag gtcgacgcag
     2221 tacaaccaat catgcttctt ctggatctca ctgaggatct tcaatctcgt tggatcggaa
     2281 agggccttgg agatcttttc cgcctgtttc gcgtccatct gcggcatatg acaaactatt
     2341 tatacctaag atacgtaatt ccgcccaatc cttcaatccc ctgatttccc taacttgcaa
     2401 ccatgaattt ccatctgacg cagatacccg aaagaacgac aagacccagg acctacggcc
     2461 tcaccatggt caacgacaaa ggcctcagcg tcgccgaagc ccgcaacctc gtttcaggcg
     2521 cttcccccta tgtcgacctg gtgaagctgg ccttcggcac ccccctcgtt accgccggcc
     2581 tccacgaaaa gatccgcctc ttccaggatg ccggcatccc cgtctatttc ggcggcctgc
     2641 tcctcgaagc cttcctcatc cgcaaccagt tcaacgatta cctttccctg ctgaaagaat
     2701 acaatatcac taccatcgag gtgtcggatg gctccatcga catccccc
//
LOCUS       DATGFG010000618         9485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1392663, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000618 DATGFG010000000
VERSION     DATGFG010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392663"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VKQ52_11535"
     CDS             <1..1127
                     /locus_tag="VKQ52_11535"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HLZ87870.1"
                     /translation="KALALHRNKEIKGLDVSLNLVTDSSAVFNVVIDPRSGDALNVRG
                     RSNLVFQLEKSGKMDLTGSYEVNSGYYSLSFNLLKRKFAIDRGSIITWTGDPTTATVS
                     LTASYTALTPSIDLISNEIAGLSSTDQNKYRQKLPFLVTLKMEGDLMKPTITFDISLP
                     TNVLALWPDVDQRLTQLRYQQSEMNKQVFALLLLGRFVGEDPLASAVGGGASPGNLAF
                     SSASQILTNQMDQLAASLIKGVDIHFDLNNQQDYSTGYEIDYTELDVTLSKQLMDERI
                     RVSVGSNFDVVGSGAPSQAPSNLAGNVAVDYKLSRDGRYLLRAYRSNQYQAVILGQVV
                     ETGVGFVLTFDYDRFKEIWHRAKGETIQERKTSKSSNSVQ"
     gene            1124..3454
                     /locus_tag="VKQ52_11540"
     CDS             1124..3454
                     /locus_tag="VKQ52_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLZ87871.1"
                     /translation="MMTGYKRMLRLFFPLGLLLAGLASCHESRYLTVNQTLYTANKTQ
                     VQSSAPMTKKEQKKWAEDMEGYLRPKLNGKTLGVRLKLWIYNIAGTTHKTKGFKHWLK
                     YKVGEPPVLASPAILQNNAAVLQSHLENKGFFHDTVIVTTLVKDKHLTATYTAQIGPR
                     YTIRNIAYPDDSDAVSNIIDTMQRRSLLKKGDFYDLETIKDERVRIDDRLKNRGFYYF
                     NPDYLLAELDTAVGNHQVDMTMRLKEEMPDPARQLYHIDKVTVYAHYDNHSDTNAKHA
                     YNTPEGYHIIDTAHYLKPVVFSKTLVIKPGDIYRVNDHNTSLSRLVSLGVFQFVKARF
                     EPASTADTNSRLDVFYYLTPSQKKQLRFEVTALTRSDNTTGTEVSVNWRHRNLFRGAE
                     LFTAKVYGGLEGQSVGFTDTTHLHRKVVLTRRAGIDLNLYFPRIIAPFNLNASSKFVP
                     KTRLQAAYDIYENSSEYTLTSSRVSFGYQFKNRITSENGITVLGINYVRPTHIDPAYQ
                     DTLNKYLNLARAIEKQFIIGPSWNFNYNSILDPRNARRPNNFWFNANVDFSNNILGIL
                     TGANVNTTGKQKEIFNVPFAQYMRFETDFRHYLSLSKYNQLVTRVNAGIGIAYGNSTT
                     MPFIKEFFAGGTNDIRAFRARALGPGSYYAGDPNRDPNIPDQPGDIKLEFNLEYRAKL
                     FGFVRWAIFTDWGNVWTLHKDTSRPGAVFTANFLNDFAVGAGTGLRFDLTIVVLRIDI
                     AVPLRTPWKPQGSKWNFKSATDISDMVLNLAIGYPF"
     gene            3540..3746
                     /locus_tag="VKQ52_11545"
     CDS             3540..3746
                     /locus_tag="VKQ52_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87872.1"
                     /translation="MKDQKRTQPKNPAIHDPSRIQHKTPRKASIENPDKNDQPDPGKE
                     IRIDDDPDQTKRKIPVSEDPRRKE"
     gene            3758..4243
                     /locus_tag="VKQ52_11550"
     CDS             3758..4243
                     /locus_tag="VKQ52_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HLZ87873.1"
                     /translation="MKTKLYASGKKTLHVLNDLIRINIDLIAGFEKAAHEEKTPEPEL
                     RDAFYRLAVESRSYVNALHAQVIRLGGAPVTQATITGKIYLHWLEGKNRFDGADSQLR
                     LAACRASAEATETAYRQALDEHLSHDLHQLLENQLWALERAHQQLHDLESSITQHDQH
                     H"
     gene            4227..4565
                     /locus_tag="VKQ52_11555"
     CDS             4227..4565
                     /locus_tag="VKQ52_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87874.1"
                     /translation="MINTIDLAECEVLLPGDWVQKGSGDIYSFTSDKMELRDQRLFRE
                     LYILEKGKPREQPIPYALTIKDDYCGILVGEEEFIILSITGEVNGSATMEWQDAAGRT
                     LGFDRAAAPA"
     gene            4922..5398
                     /locus_tag="VKQ52_11560"
     CDS             4922..5398
                     /locus_tag="VKQ52_11560"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLZ87875.1"
                     /translation="MKNALFFSLNTSLLRIATTIVSVIKVDELGQMWFFVPRPQQALH
                     EFDREFPVRLEFFRKGKEFFLHVSGKAFIVTDPEEINSLVHEDIRELTGNHLVLIRVK
                     MLKADYFGNAHSSLHAGWWHDLKNQLHTWMFNTRPGYKPYYLADAPLPQLTYTPVA"
     gene            5526..6146
                     /locus_tag="VKQ52_11565"
     CDS             5526..6146
                     /locus_tag="VKQ52_11565"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HLZ87876.1"
                     /translation="MKNFPTYLAIGLFTAFIGMGCGGGASNDSTQAAKDSNATKMDST
                     GTDTTGAGKMASIPPTVSQADQKFAVDAANAGMTEIQAAKMAQQQGTEADVKSYAAMM
                     IKDHTAAANRLKAVAAKKNLTLPSDISPEMQQNLTDLQKKTGKTFDQAYMKMMVSDHK
                     KAVSAFEDEAKNGSDADIRAFADSTLHTLHHHLDEAQKCEKMLSKM"
     gene            6311..6850
                     /locus_tag="VKQ52_11570"
     CDS             6311..6850
                     /locus_tag="VKQ52_11570"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLZ87877.1"
                     /translation="MSEKNFTLSHRDGTFFRKDNTFGVCFERILDHPVSIVWKAITDP
                     AQLPKWLAPVIIKDDTISLQLTGGTMGGRILQWKENTLLEYEWYEGSIVRWELVTEGP
                     GGCRLVFTHRNVMQSQLQGAATGWHYHMDILGIILDGDTPPQNPVKRWEAISRDAAAR
                     YKAALQRFEGRPSIPFIRN"
     gene            complement(6852..9341)
                     /locus_tag="VKQ52_11575"
     CDS             complement(6852..9341)
                     /locus_tag="VKQ52_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87878.1"
                     /translation="MFHNHLVIAWRNLLRNKTNSFINIGGLAIGITCVLFIVLYVQDE
                     LSFDRGFAQADRIYQVNMETNFGGLEANTSNTPSPVAISLHNSFPEVVAYTRLYQPGN
                     MVVHNDVAADVAGHSFTEKRIWAADSNFLQVFSFRLIAGDRATCLKKYHSIVLTESMA
                     RKYFGDGQAIGRTLTLDTAAAPYVVTGILRDLSTNSSLQFDALTPLQDNALVQHFSWS
                     WVWTQLQTYVVLNPETAGDPAAIRRLEAKFPVMVRRDAARAFARIGQPFDEFIRKGGK
                     WDFYLQPLTRVHLYSAGTGTPYTNLGSIRYVYIFSVIAVFIILLACINFMNLSTAQAA
                     KRAKEVGIRKVLGSMKAQMVRQFLSEALLYTALASALAVGLLYVLMPAFDVVAGKQLN
                     FTDLFRHGSWVIILFVLLVTGLLAGSYPAFYLTSFKPVDVLKGGGLFARSLGNQLIRN
                     GLVVFQFTVSIALMISTLIVYRQLQYMNTIDLGFNKDHVVIFPNVEKLPNGSEKALRE
                     QLSGIPGMRAVSVSSGMPANMATIFIDFYVPVKTGVPEPLARDVSLTSYLVDEYFVPS
                     LQMQLIAGRNFSKEFHDSASVIVNEATVRQIGWKSPLGKRIRYPGNDDQTFTVIGVVR
                     DFNFQSLRDTVVPFALFHTSSMTFKPTTNYIVASVDGRHTAAALQGAERVWKRWVPAV
                     PFEYSFLDRDYAALYQSEEKMGHVFGVFTSLSILVACLGLFGLSVYTAERRVKEIGIR
                     KILGASARQVVGLLSKEFLKLVCLSTLIAFPLAWWAMSRWLMDFAYRTPMSAWLFVLA
                     GAVALAIALLTVSGQALKAAMRNPARSLRSE"
BASE COUNT         2439 a         3005 c         2191 g         1850 t
ORIGIN      
        1 gaaaggcgct ggccctgcac cggaacaagg aaataaaagg cctcgacgtg agcctgaacc
       61 tcgtcaccga cagcagcgcg gtcttcaacg tcgtgatcga cccgcgaagc ggggacgccc
      121 tcaacgtccg tggccgcagc aacctggtct tccagctgga gaagagcggc aagatggacc
      181 tcacgggctc ctacgaggtc aacagcggct attatagcct ttccttcaac ctgctcaaac
      241 gcaagttcgc gatcgatcgg ggtagtatca tcacctggac cggcgacccc accaccgcaa
      301 ccgtcagcct gaccgcctcc tacaccgccc tgaccccatc catcgatctc atctccaatg
      361 agattgccgg cctttcctca accgatcaga acaaataccg gcaaaaactg cccttcctcg
      421 ttaccctgaa gatggaggga gatttaatga aaccaaccat cacctttgat atttcgctgc
      481 ccaccaacgt cctggccctc tggcccgacg tcgaccaacg tctcacacag ctgcgctatc
      541 aacagtccga aatgaataaa caggtcttcg ccctcctgct gctcggccgc ttcgttggag
      601 aagatcccct ggcttccgcg gtgggcggcg gcgccagccc cggcaacctg gctttctcga
      661 gcgccagcca gatcctcacc aaccagatgg accagttggc cgccagcctc atcaaaggcg
      721 tcgacatcca tttcgacctc aacaaccagc aggattacag cactggctat gagatcgatt
      781 acaccgaact ggatgtcacc ctttccaaac agctgatgga cgagcggatc cgcgtcagcg
      841 taggcagtaa cttcgacgtc gttggaagcg gcgctccgag tcaggcccct tccaacctcg
      901 ccggcaatgt cgccgtagac tataaactgt cccgggacgg ccgttacctg ctccgggctt
      961 atcgctcgaa ccaataccag gcagtaatcc tgggacaggt ggtagagacg ggcgtaggct
     1021 tcgtcctgac cttcgactac gacaggttca aagagatctg gcatcgcgcc aagggggaaa
     1081 cgatacagga aagaaaaaca tccaaatcca gtaattctgt tcaatgatga caggctataa
     1141 acgcatgctc aggcttttct tccctttggg cctcttgctg gccggcctcg cgtcctgcca
     1201 tgaatccaga tatctcacgg taaaccaaac gctgtacacg gccaataaaa cgcaggtcca
     1261 gtcctctgcg ccgatgacaa agaaagagca aaagaaatgg gccgaggaca tggagggcta
     1321 cctccggccc aagctcaatg gcaaaaccct cggcgtccgg ctgaaactct ggatctacaa
     1381 catcgccgga acgacgcata agacaaaagg cttcaaacac tggctgaaat ataaagtagg
     1441 agagcccccc gtcctggcat ctccggcgat cctccagaac aacgcggcgg tcctgcaaag
     1501 ccacctcgag aacaaaggat tttttcacga tacggtcatc gttaccaccc tcgtaaagga
     1561 taaacacctt acagccacct acaccgcgca gatcggtccc cgctacacca tccgtaacat
     1621 cgcctatcca gacgactccg atgccgtcag caacatcatc gacacaatgc aacgccgctc
     1681 cctgctgaaa aaaggcgact tctacgatct cgagacgata aaggacgaac gcgtgcggat
     1741 cgacgaccgg ctcaagaacc gcggcttcta ttatttcaac cccgactacc tcctcgccga
     1801 gctcgacacc gccgtcggga accaccaggt ggatatgacc atgcggttga aagaggagat
     1861 gcccgatccc gcccgccagc tctatcacat cgataaggtc accgtgtatg cccactatga
     1921 caaccactcg gacaccaacg ccaaacatgc gtataacact cctgaaggct accacatcat
     1981 cgataccgcc cattacctca agcccgtcgt ctttagcaag acgctcgtga taaaacccgg
     2041 cgatatctac agggtcaatg atcacaacac ctcccttagc cgccttgtca gcctgggcgt
     2101 cttccagttc gtaaaggccc gcttcgagcc cgcctcgact gccgatacca acagcagact
     2161 cgacgtcttt tactacctga cgccttccca gaagaaacaa ctgagattcg aggtcaccgc
     2221 cctcactcgc agcgataaca ccaccggtac cgaggtctcg gtcaactggc ggcatcgcaa
     2281 ccttttccgc ggcgcggagc tgttcaccgc aaaggtctat ggcggcctgg aaggacagtc
     2341 ggtaggcttt accgatacaa cccatcttca cagaaaagta gtcttgaccc gccgcgcggg
     2401 gatcgacctg aatctgtatt tccctcgcat cattgccccc ttcaacctca acgccagcag
     2461 caaattcgtt ccaaagaccc gcctccaggc ggcctatgat atctatgaga acagctccga
     2521 atacaccctc acctcctccc gcgtcagctt cggttaccag ttcaagaatc gaatcacctc
     2581 cgagaacggc attacagtcc tggggattaa ctatgtccgg cctacccata tcgacccggc
     2641 ctatcaggac accctcaaca aatacctcaa cctggcccgg gccatcgaaa agcagttcat
     2701 catcgggccc agctggaatt ttaactacaa ttccatcctc gatccccgca acgcccggcg
     2761 accgaataat ttttggttca acgcaaacgt cgacttctcc aataacatcc tcggtattct
     2821 caccggcgct aacgtcaata ctacggggaa acaaaaagag atcttcaacg tgccctttgc
     2881 ccagtacatg cgcttcgaaa ccgacttccg gcactacctt agcctcagta aatacaacca
     2941 gctggtcacc cgcgtcaatg ccgggattgg catcgcctat ggcaatagca ccaccatgcc
     3001 ctttataaaa gagttcttcg ccggcggcac caacgatatc cgcgccttcc gcgcccgcgc
     3061 cctcggcccg ggcagctatt atgccggcga tcctaaccgc gatccaaaca tccccgatca
     3121 acccggagac atcaaactgg agttcaacct ggaataccgc gcaaagctct tcggcttcgt
     3181 ccgctgggcc attttcaccg actggggtaa cgtctggacc ctccataaag ataccagccg
     3241 ccccggagcg gtcttcacag ccaacttcct caacgacttt gccgtcggtg ccggcacggg
     3301 cctccgattc gacctcacca tcgtggtcct ccgcatcgac atcgccgtcc ccctccgcac
     3361 tccctggaaa ccccaaggct ccaaatggaa cttcaaaagc gcaacggaca tctccgatat
     3421 ggtcctcaac ctcgccatcg ggtatccctt ctgatctacc tgacggcaat attttccccg
     3481 cattctcacc gcacccggcg atggcacatt tttttcggcc ttatttgaaa actcaggtta
     3541 tgaaagatca aaagagaacg cagccgaaga atcctgctat tcacgatcct tccaggatcc
     3601 agcacaagac accccgcaag gcgtccatag agaacccgga caagaacgac caacccgatc
     3661 cgggaaaaga aataaggatc gacgacgatc ccgaccagac aaaaagaaaa attcccgtca
     3721 gcgaagatcc gagacggaaa gaataaaacc gatcactatg aaaacaaaac tctacgccag
     3781 cgggaaaaag accttacacg tattgaatga cctcatccgc atcaatatcg atctcatcgc
     3841 tggctttgaa aaagcggctc atgaagaaaa gacgccggaa cccgaactcc gcgacgcctt
     3901 ttaccggctg gctgtcgaaa gcaggtccta tgtcaacgcc ctgcacgcac aggtcatcag
     3961 gctgggcggc gcacccgtga cccaggccac catcaccggt aagatctatc ttcactggct
     4021 ggaaggaaag aatcggttcg atggggccga cagccaatta aggctggcgg cctgtcgcgc
     4081 ttccgccgag gcgaccgaaa ccgcctatcg gcaagccctc gacgagcacc tgtcacatga
     4141 tctgcatcag cttctcgaaa accagctttg ggccctcgaa agggcccatc aacaattgca
     4201 cgacctggaa tcctcaataa cacaacatga tcaacaccat tgaccttgcg gaatgtgaag
     4261 tcctcctgcc cggcgactgg gtccaaaaag gatcgggcga tatctactcc ttcacatcgg
     4321 ataaaatgga attgcgcgac cagcgcctgt tcagagaact gtatatcctc gaaaaaggaa
     4381 aacccaggga acaacccatt ccctatgccc tgaccatcaa ggacgactac tgcggtatcc
     4441 tcgtcgggga agaagaattc atcatcctca gcataacggg cgaggtcaac ggctccgcaa
     4501 cgatggaatg gcaggacgcc gccggccgca cactcggatt cgatcgcgcc gctgccccgg
     4561 cctgaaacgc cgctgcccat gcttgaaaca ccaataataa aaccccgtgg aaacggggtc
     4621 tctggtatag tattctttca ggtttattga cccggttata tattaaaaaa ttgtgccacc
     4681 tcgttcgtcc gtcgtatcat cccggtttac aatgggtatt tttttcctca ccctccccat
     4741 tgaccggtcc cgatgcctcc gaagcgattt cccttgataa tgagattctc ctgtaatatc
     4801 cacaccccgc tcactgcccc ttttaacact ggtactgaat ttgaaaaaat cactggtaat
     4861 aaaatgacta cttatgttgc cccaatcgga cctgcaattt ctgcagcaaa aaatacagga
     4921 tttgaaaaat gccctattct ttagcctgaa cacctctctg cttaggatcg caacaaccat
     4981 tgtttcggtg ataaaagtgg acgaattagg acaaatgtgg tttttcgttc cccgccccca
     5041 acaggcttta catgagttcg acagggaatt ccccgttagg ctcgagttct tccgtaaagg
     5101 aaaggaattc tttctccatg tctcgggtaa ggcctttatc gtcaccgacc ccgaggagat
     5161 caacagtctc gtccacgaag acatccgcga actcaccggc aaccacctcg tgctcatcag
     5221 ggtaaaaatg ctgaaagcgg attacttcgg gaacgcccac tcctcgttgc acgccggctg
     5281 gtggcacgac ctgaagaacc agctgcatac ctggatgttc aacacccgcc ccggttacaa
     5341 accctactat cttgccgacg ctcccttacc ccagcttaca tatactcccg tggcataacg
     5401 cccgtttcgt atccttgctc cgtaccctct ctttcttact tcccatatcc ttcgtcgcat
     5461 aaccatcctc catggcataa caattgaagc actatcggtg agtcattact gaaaaatagc
     5521 cgattatgaa aaacttcccg acgtatctcg ccattggact cttcaccgcc ttcatcggca
     5581 tgggttgcgg cggcggcgcc tccaatgaca gcacacaggc agcaaaagat tccaacgcaa
     5641 caaaaatgga cagcaccggc accgatacca ccggcgcagg taagatggct tctatacccc
     5701 caaccgtatc ccaggccgat cagaaattcg ccgtggacgc cgcaaatgcc ggcatgaccg
     5761 agatccaggc cgcaaaaatg gcccagcaac agggaaccga agccgacgtt aagagttacg
     5821 ccgccatgat gataaaggac cataccgcgg ccgcgaacag gttaaaggcg gttgctgcca
     5881 aaaagaacct taccctgcct tccgatatct cgccggaaat gcagcagaac cttaccgatc
     5941 tgcaaaagaa gaccggcaaa accttcgacc aggcctatat gaaaatgatg gtctccgatc
     6001 acaaaaaggc ggtcagtgcc tttgaagacg aagcgaaaaa tggctccgac gccgacatcc
     6061 gtgccttcgc tgacagcacg ttgcacaccc tgcatcatca cctcgacgaa gcgcagaaat
     6121 gcgaaaagat gctatcgaaa atgtaatagg atcaggtacc gttaactacc attcggatct
     6181 tacccagtga acaggcgggc gggaaattcc cgcccgttct gctttttttc agcccccact
     6241 ctttgtgaaa ccaaaaggtt tcctatattt gcaacgaaag ggtttcctat ttatcttaaa
     6301 atcgtttgtt atgtccgaaa aaaacttcac tctctcccac agggatggga ccttcttccg
     6361 aaaggacaat acctttggcg tttgcttcga gcgcatcctc gaccatcccg tgtccatcgt
     6421 atggaaagcg atcaccgacc cggcccaact ccccaaatgg ctggcgccgg tcatcataaa
     6481 agacgacacc atctcgctcc agctcaccgg cgggacaatg ggcggcagga tactgcaatg
     6541 gaaagagaac accctgctcg aatacgaatg gtatgaaggc agtatcgtcc ggtgggaact
     6601 ggtcaccgaa ggcccgggcg gctgccgtct cgtctttacc catcgcaacg tcatgcaatc
     6661 ccaactccag ggcgcagcca ccggctggca ctatcatatg gatatcctcg gcatcatcct
     6721 cgatggcgat actccaccgc aaaaccccgt caaacgctgg gaagctatct cccgcgatgc
     6781 cgccgcccgc tacaaagctg ctttacagag atttgaaggc agaccatcca tcccctttat
     6841 aaggaactga atcactcgga ccgcaaactc ctggcaggat tacgcatcgc cgccttcagc
     6901 gcctgcccgc ttaccgtcag caacgcaatg gccagcgcaa ccgcgcccgc cagaacgaat
     6961 agccaggcac tcatcggcgt gcgataggca aaatccatca gccatcgcga catcgcccac
     7021 caggccagtg gaaaggcgat cagcgtggaa agacaaacca gcttcaggaa ttccttggac
     7081 aaaagcccaa ccacctgccg cgcactggcg ccaaggatct ttcggatacc gatctccttg
     7141 acacgccgct cggccgtgta gaccgacaac ccaaaaagcc ccagacaggc cacaaggatc
     7201 gacagactgg taaacacgcc aaagacatga cccatctttt cctccgactg gtacagagcc
     7261 gcataatccc tgtccaggaa agaatattcg aaaggaacag ccggaaccca ccgcttccat
     7321 acccgctccg ccccctgtaa tgccgctgcc gtatgccggc catccacact ggccacgata
     7381 tagttcgtgg tcggtttaaa cgtcatgctg ctggtatgaa aaagcgcaaa cggcacaacc
     7441 gtgtcccgca gtgactggaa gttgaagtcc ctgactaccc cgatcaccgt aaaggtctgg
     7501 tcatcgttcc ccgggtaccg gatgcgtttg cccagcggac tcttccaccc gatctgccgg
     7561 acggttgcct cattgacgat cacggacgcc gaatcgtgga attcctttga aaaattccgc
     7621 cccgctataa gctgcatctg cagcgacgga acaaagtatt cgtcgacaag atacgaggtc
     7681 agcgaaacgt cccgggccag cggctccggg acccccgtct tgacagggac ataaaaatca
     7741 ataaagatag tcgccatatt ggccggcatc cccgatgaca cgctcacggc gcgcatcccc
     7801 ggtatcccgc taagctgctc ccgcaaggcc ttctccgacc cattgggtaa cttctccaca
     7861 ttgggaaaga taaccacatg atccttgttg aacccaagat cgatggtatt catatactgc
     7921 agttgccggt agacgatcag cgtcgagatc atcagggcga tcgagacggt aaactggaaa
     7981 acgacaagcc cgttgcggat aagttggttg cccaggctcc gggcgaataa ccccccgccc
     8041 ttcagcacgt ccacgggctt gaaagaagtg agataaaaag caggatagct cccggctagc
     8101 agccccgtca ccaacagcac aaaaaggatg atgacccagg aaccatgacg gaaaaggtcg
     8161 gtaaagttca actgcttccc cgccacaaca tcgaaagcag gcattaatac atacaaaagg
     8221 ccgacagcca gcgcggaagc cagcgccgta tacaacaaag cctcggacag gaactggcgc
     8281 accatctgcg ccttcatcga tcccaacacc ttccggatcc ctacctcctt cgcccgtttc
     8341 gccgcctgcg ccgtcgacag gttcataaaa ttgatacagg ccagcaggat gataaagacg
     8401 gcgatgacgg aaaagatata gacataccgg atactgccca ggttcgtata gggcgttccg
     8461 gtccccgcgg agtagaggtg gaccctggtc aacggttgca gatagaaatc ccattttccg
     8521 cccttcctga taaattcatc gaagggctga ccgatccgcg caaaggcccg cgccgcatcc
     8581 cggcgcacca ttacaggaaa tttggcctcc agccgtctga tcgccgccgg atccccggcc
     8641 gtctccgggt tgagcacgac ataggtctgc aactgtgtcc aaacccagct ccaactgaaa
     8701 tgctgcacca gcgcattgtc ctgcaacggc gtcaacgcat cgaactgcaa cgaggagttg
     8761 gtggacaagt cccggagaat acccgtcact acatagggcg ctgcggccgt gtcgagggtc
     8821 agcgtccgcc caatggcctg cccatcacca aaatatttgc gggccatgga ctccgtaagc
     8881 acgatcgaat gatatttctt caggcaagtg gcccggtccc cggctataag ccggaaagag
     8941 aatacctgca aaaaattcga atccgccgcc cagatccgct tttccgtgaa agaatgcccc
     9001 gcgacatccg ccgctacatc attgtgcacc accatattcc ccggctgata cagccgggta
     9061 tacgcaacca cttcggggaa actgttatgc agggagatcg ccaccggcga aggcgtattc
     9121 gacgtattgg cctccagtcc gccaaaattc gtctccatat tcacctggta gatccggtcg
     9181 gcctgggcaa atccccggtc aaaactcagt tcgtcctgca catacaggac gataaaaagg
     9241 acacaggtga tcccgatggc aagaccgccg atgttgataa aactgttcgt cttattgcgc
     9301 agcaggtttc gccaggcaat gacaagatgg ttatggaaca tattgataag attggactac
     9361 tcggtcttca gcatctgcga ctactccgtc ttcagcgtct ttgcgggatt ctccctcgcc
     9421 gccctcatcg cccgcagccc caccgtcagc accgcgatca cgacggccag caaacctgct
     9481 gcggc
//
LOCUS       DATGFG010000619         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1591637, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000619 DATGFG010000000
VERSION     DATGFG010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591637"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VKQ52_11580"
     CDS             complement(<1..952)
                     /locus_tag="VKQ52_11580"
                     /inference="COORDINATES: protein motif:HMM:NF016159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HLZ87879.1"
                     /translation="MLSKQEQYLDNESTVRLAEALLGRFTHCHERYAFYHASSLEFNE
                     VYSFVRAIFEDPGVLYEQSVRIASHLFEVSLHPKIKPGELYVGYFGNIQDDGAVMDAV
                     GIFKAETKTLFADLIPGEDDLDLQFKEGVELSKVDKGCLILKRGEENGYELLVFDANG
                     KGEEAQFWKDKFLGVLIRQNEFVQTQGFLTLTKNFVTQQLPGEFEVSKTEQIDILNRS
                     MDYFKTHGNFDRNEFESEVLHHEDLIQSFRNYDEQYRKNNDLDPLDSFEISSHAVKKQ
                     ARVFKSVIKLDKNFHIYVHGNRELIEKGFDEATGKHYYKIY"
     gene            1542..>2726
                     /locus_tag="VKQ52_11585"
     CDS             1542..>2726
                     /locus_tag="VKQ52_11585"
                     /inference="COORDINATES: protein motif:HMM:NF020698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HLZ87880.1"
                     /translation="MTLKMNNNDVLTLAPGSNTRRPFIGMVMLSIGLLLGFSLFGFST
                     PRNPASPGKPADLYIFTTTVTVNDQVADGVSNDIIRVYVTYATGPLAGQPADGVSVNF
                     QPQGAAISSSRTTGPASFPSGYVDFPVTSTATGTSPVKVTIGGVAAPQFVEVTFVPGP
                     PDPSTSYLLVVQDNAAANGTSDDIVKAFLYDAWGNTVADSTPVSFSIPGGTTLAATSK
                     THLGAAYAYYTSPTAGTYQVGATINGGQPLTDGSGNLTVPIHFVAGPPNPNPPPGSTN
                     PSYYKTIIQSAPADNSTPTQVELHATDGTNNEPIGTPVKFVITSNTPASGPAVMNGVS
                     GGTYTSSLPDNSGTIDVSIKDGTAGDVTIQAFVWDITTNQWISFGSQTVTFVVAPPNP
                     NPP"
BASE COUNT          677 a          828 c          575 g          646 t
ORIGIN      
        1 aatagatctt ataatagtgc tttcctgtcg cctcatcaaa ccccttctct atcagctcac
       61 ggttaccatg cacataaatg tggaagttct tatccagctt gatcacgctc ttgaacaccc
      121 gcgcctgctt cttcactgca tgtgatgaga tctcgaagga atcaagcggg tcgagatcgt
      181 tgttcttccg gtactgctca tcataattgc ggaaagactg gatcagatcc tcatgatgca
      241 gaacctcgct ctcgaactcg ttgcgatcga aattcccatg cgtcttgaaa taatccatgc
      301 tccggttgag gatatcgatc tgttccgtct tggatacctc gaactccccc ggcaactgct
      361 gggtgacaaa attcttcgtc agggtcagga atccctgcgt ctgcacgaac tcattctgcc
      421 ggatcagcac gccaaggaac ttatccttcc agaactgcgc ctcctctccc ttgccattgg
      481 catcgaagac aagtaactcg tacccgttct cctccccccg cttaaggatc agacatccct
      541 tatccacttt cgataactcc actccctcct tgaattgcag atcgagatcg tcttcccccg
      601 ggataagatc agcaaaaagc gtcttcgtct ccgccttgaa gatacccacc gcatccatca
      661 ccgccccatc atcctggata ttaccgaaat atcccacata caactccccc ggctttatct
      721 tgggatgcag cgacacctcg aaaagatgag aagcgatccg caccgactgc tcataaagca
      781 cacccgggtc ctcgaatatc gcccggacaa aactatacac ttcattgaac tccagcgaag
      841 aggcatggta aaaagcataa cgttcatgac aatgggtaaa ccgcccaagt aaggcctccg
      901 ccaggcgaac ggtcgactcg ttatccaggt actgctcctg tttcgacagc aaaagcgccc
      961 ctccgttttg cttatttccg gtataatgta cagtcaatct gtcaattgta gcctctgtaa
     1021 agatcatcgt aaaaatcgta aagatagggt tctctcccgg ctcgcggaaa atgtcctaac
     1081 tgtttgactt tgatcaaagg ttcatccgcg cacggtagtt ttcccttaga aaatctaaaa
     1141 aatgtggata actaataaaa ctaacgaaaa aggacaaatg ctttaattac caggtcttta
     1201 tgagataaat acaatttcgt caatccaccg gtgtcaaatt tatcctagcc ccagtacaat
     1261 aaaaaggggg taaaaatcct ttttataata gtataaatcc gatatcccga tagaaagacc
     1321 ttcaagctaa aacccaatta ttatgaaacc caaaccttac acaaactttt taccgctccg
     1381 gctcgttgcc cgaaagtgac aagcacttag tgtctcctca tgctgtcacc gcaacgactt
     1441 ctgtaggtgt cctgtcaccg tttttcctac tgtacaaaac aagttgccgt gtctccatga
     1501 gaccgtaaag attattgtgt agggtgaaat ttccgtgttc tatgacccta aaaatgaata
     1561 ataacgatgt tctgacgctt gcccctggta gcaatacccg gcgtcctttc atcggtatgg
     1621 tgatgctgag cattgggctg ttactgggct tttccctgtt tggcttctcc actccccgaa
     1681 acccggcatc cccgggcaaa cccgccgacc tgtacatttt caccaccacc gtgaccgtga
     1741 atgaccaggt tgccgacgga gtctcgaatg atatcataag agtatatgtc acctacgcaa
     1801 ccggccccct cgcgggacag ccggccgatg gcgtatctgt caattttcag ccgcaagggg
     1861 ccgccataag ctcttcccgt acaacgggcc ctgcgtcctt tcccagcggc tacgtagact
     1921 ttcccgtaac cagcaccgcc accgggacct ctccggttaa agtcactatc ggcggcgtgg
     1981 ccgctcccca gttcgttgag gtgaccttcg ttccaggtcc gccggatccc agcaccagct
     2041 atctgctggt agtccaggac aacgcagcgg ccaacggcac ctccgacgac atcgtgaaag
     2101 cctttctcta tgatgcatgg ggcaacacgg tggccgatag cacaccggtg agctttagca
     2161 tccccggagg aacaacattg gccgccacct ccaagacaca tctcggcgct gcctatgcct
     2221 actacaccag ccctaccgcc ggtacctacc aggtgggcgc caccatcaat ggcggtcagc
     2281 cgctcacgga cggaagcggc aacctgacgg tccccatcca tttcgtcgcg ggcccgccaa
     2341 atcccaatcc tccgcccggt tcgaccaatc cttcttacta taaaacgata attcagtcgg
     2401 cccctgccga taattcaact cctacccagg tggagctcca tgccaccgac ggtacgaata
     2461 atgaacccat cggtacaccg gtgaaatttg tcatcaccag caacacgcct gcatcaggtc
     2521 cggcagtaat gaacggggtt tccggcggca cctataccag ttcgcttccc gacaacagcg
     2581 ggaccatcga cgtctccatc aaggacggta ccgccggtga tgtgaccatc caggccttcg
     2641 tctgggatat tacgaccaac caatggatat cctttggatc gcagacggta acctttgtcg
     2701 tcgctccccc aaatcccaat cctccg
//
LOCUS       DATGFG010000620         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1261451, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000620 DATGFG010000000
VERSION     DATGFG010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261451"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..1017
                     /locus_tag="VKQ52_11590"
     CDS             250..1017
                     /locus_tag="VKQ52_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ87881.1"
                     /translation="MAAKTIISVNNLTKKYGDFEAVKGISFEVQEGEIFGLLGPNGAG
                     KSTTLEIIETLRDKTSGKVTVDGLDLDKDPGEIKKIIGVQLQTSGYYPGLNLSELILL
                     FGGLYNQEVDPLELLDRVNLRDKARAKFKELSGGQKQRFSIATTLINEPRIIFLDEPT
                     TGLDPQARRNLWELIKEIRAKGTTVIITTHYMDEAEILCDRVAIIDSGKIIALESPDR
                     LIDKLVAEGFERPKEVKKANLEDVFIQLTGHALRPEN"
     gene            1075..1611
                     /locus_tag="VKQ52_11595"
     CDS             1075..1611
                     /locus_tag="VKQ52_11595"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HLZ87882.1"
                     /translation="MSITTTDLRYPIGRYEPKPYSEHQKQEWLNDIKFLPQSLENAIH
                     NLDEAQLQTPYREGGWTAQQVVHHVADSHINAYCRFRLGLTEDKPAIRPYDEKAWALL
                     NDVQTIPINVSITLLYALHTRWYATIADLTLKQWEREVYHPEAEKVMTLWYLLGNYAW
                     HSRHHVAHITSLRERNHW"
     gene            1668..2225
                     /locus_tag="VKQ52_11600"
     CDS             1668..2225
                     /locus_tag="VKQ52_11600"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLZ87883.1"
                     /translation="MKDLSWKILSSEYLFKESWFTLRSDTCETQDGKIIKPFYVYEFP
                     TWVIAVALTADGRFILERQYRHALGVTAMELAGGCVDPTDATLEDALRRELLEETGYR
                     FEAVEYLGKTSANPSTNNNWAYFYLATGGQRVQEQHLDFNEEIEVCLYTLDELKQLLR
                     DEGIVQSMHVAALFYALTRLGHLHW"
     gene            2729..>3088
                     /locus_tag="VKQ52_11605"
     CDS             2729..>3088
                     /locus_tag="VKQ52_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87884.1"
                     /translation="MLRHYFRIALRNLAKQKVLTFINVAGLSIGLACFSLILLFAVSE
                     FNYDKFNTRADRIFRVAQLATRDDGQDMGTVDLNVALGPTMKKAFPDVEEAVRISHGD
                     KKFMRGNNGISLLDMFFA"
BASE COUNT          692 a          863 c          906 g          627 t
ORIGIN      
        1 ccggtccgat gtcggcacgg gaagtggtgg acggctggct acacagcccc ggacaccgcc
       61 gcaacattga aggggatttt aacctcaccg gtatcggtct tgccttcggc cgcgatggga
      121 acatcttctt tacgcagata ttcgcgaaat aattgcctaa ttcaccatcc cgcgatggcg
      181 ggggagaggg taggcgccgc ctgcataaat tggccccgcg aaaaaccgcg ttttccgtaa
      241 cttgctccca tggcagcgaa aacgatcatc tccgttaata atctcacaaa aaaatacggc
      301 gacttcgaag ccgtcaaggg catcagtttc gaggtacagg aaggcgaaat attcggtctg
      361 ctcgggccca acggcgccgg taaatcgacc acgctcgaga tcatcgagac cctgcgcgac
      421 aagaccagcg gaaaagtcac ggtggatggc ctcgacctgg acaaagaccc cggggagatc
      481 aagaagatca tcggtgttca gttacagacc tcgggttatt atcccgggct caacctcagc
      541 gaactcatcc tgctctttgg cgggctatat aaccaagagg tagatcccct cgagctgctc
      601 gatcgtgtca atctccgcga taaggcgagg gcgaagttca aggaactcag cggcggccag
      661 aagcaacgct tctccatcgc aaccacgctg atcaacgaac cccggatcat ctttctcgac
      721 gaacctacca ctggtctcga cccccaggcg cggcgcaatc tctgggaatt gataaaggag
      781 atccgcgcaa agggcaccac ggtcatcatc actacacatt atatggacga agccgagatc
      841 ctttgcgacc gggtcgcgat catcgactcg gggaagatca tcgcgctgga gtcccccgat
      901 cggctgatcg acaaacttgt tgcggagggt ttcgagcgac cgaaggaagt gaagaaggcc
      961 aacctggaag acgtgtttat tcaactcacc gggcatgcgc tgcggccgga gaactaggac
     1021 gatctcgagg caatggcggc gacacgggaa atcttgcaac cataatctta gcatatgagc
     1081 atcactacaa ccgacttgcg ttatccgatc gggcggtatg agccaaagcc ctactccgag
     1141 catcagaaac aggaatggct gaacgacatc aagttcctgc ctcagtccct ggagaatgcc
     1201 atccacaacc tcgacgaagc ccagttgcag acaccctacc gtgaaggcgg ctggacagcg
     1261 cagcaggtgg tgcaccacgt ggcggacagc catatcaacg cctattgccg gttccggttg
     1321 gggctgacag aggacaaacc tgccatccgg ccctatgacg aaaaggcctg ggccctcctg
     1381 aatgatgtcc agaccatccc catcaatgtc tccatcacgc tgctgtatgc gctgcatacc
     1441 cgctggtatg ccaccattgc cgatctcacg ctcaagcagt gggagcgcga ggtctatcat
     1501 cccgaggccg agaaggtgat gacgctttgg tatctgctgg gcaattatgc gtggcatagc
     1561 cggcatcacg tggcgcatat cacttccctt cgcgaaagga atcattggta gggcgcccgc
     1621 cgcgctgatc tggggttggc gcgggcgcct caaacacaaa actttgcatg aaagatctca
     1681 gttggaaaat cctctcatca gaatatctct ttaaggaaag ctggttcacg cttcgaagcg
     1741 acacctgcga gacccaggac ggaaagatca tcaaaccgtt ttatgtctac gaattcccga
     1801 cctgggtcat cgccgtcgcc ctcaccgccg atgggcgatt cattctcgaa cggcaatacc
     1861 gccacgcgct tggcgttacg gccatggaac tggccggtgg ctgtgtagat cccaccgatg
     1921 ccacgctcga ggatgcgctt cggcgggagt tgctcgaaga gacgggctat cgtttcgagg
     1981 ctgtcgaata tcttggcaag acctcggcca atccctcgac caacaacaac tgggcctatt
     2041 tctatctggc taccggtggg cagcgggtac aggaacagca cctcgacttc aacgaagaga
     2101 tcgaggtgtg tctctatact ctcgacgagc taaagcaact gctccgcgat gagggtatcg
     2161 tccagagcat gcatgtcgct gcgctgttct atgcgctgac ccgcctcggg catctgcatt
     2221 ggtagggcag ggggtaggag agcagggcct ggattggtag gcctgagggc gtcgaggggc
     2281 ctggagtggt gggctgagag cgtcgggagg gacctggatc ggagggtaag gggctgtcgg
     2341 ggggcggccg gggttggtgg gcaagatgcc gtcgggggcg gccaggattg gtgggctgag
     2401 ggcgtcgagg ggccaggatt ggtgggcagg gggaacggcc aggatcggag ggtaagggct
     2461 gtcgggaacg gccaggattg gggcaagagg ccgtcggcgg tggctaggat tggtggtaag
     2521 gggtcatcgg gggcggccag gattggtggg cagcggtgtg cgttcccctc cgggtttccg
     2581 gattggcaat attgcctgtt acgaattatt tcgtacattt tgtgttcgaa aacgaacact
     2641 ttcgtacact ggctttcgcg ctgctccttg ataaccagca ccctgtcttt ccggcatcct
     2701 cttcgcggca tggaacaaaa ccaattctat gctccggcac tatttccgca tcgccctccg
     2761 caatctcgcc aaacaaaaag tcctgacttt tatcaatgtc gccggccttt ccatcggcct
     2821 cgcctgcttc tcgcttattc ttcttttcgc cgtcagcgaa ttcaactacg acaaattcaa
     2881 cacccgggcc gaccgcatct ttcgtgtggc gcagctggcc acccgagatg acgggcaaga
     2941 tatgggtacg gtagacctca atgtggccct ggggccgacg atgaaaaaag cctttcccga
     3001 tgtggaagag gccgtgcgca tctctcatgg cgacaaaaaa ttcatgcggg gcaacaacgg
     3061 catctccctg ctcgacatgt tctttgcg
//
LOCUS       DATGFG010000621         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_859033, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000621 DATGFG010000000
VERSION     DATGFG010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859033"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4678)
                     /locus_tag="VKQ52_11610"
     CDS             complement(<1..>4678)
                     /locus_tag="VKQ52_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLZ87885.1"
                     /translation="QGLLTGRCGLMPIQRWYFSREQDDVSYYNQRVLMGVDKNIKGEV
                     LEDAVKMLLRRHDGLRFVYHRGDEGWEQEYGTYMGRLDQEDLRNAAAGELDQAIGRRA
                     DSYQRSLDIGKGELVRVVWMRTPDWEQDDRLLIVIHHLAVDGVSWRILLEDLEELLAK
                     GAAEPGRKTSSYREWRERLVAYGSSRRVQQQRKYWEEVLGSYRALEVEWSDGAREVRI
                     EDMGSYRVRLDKEQTRRLLQELPRVYHTEINDVLLAALAQTICDWQSREKITIGLEGH
                     GREEGLFEGVDVSRTVGWFTSLYPVALEVERGLAAGELLKAIKEQLRRIPDKGIGYGV
                     LGYEQTAWDIRFNYLGQLDNATRNNRWLRDVGGPAGESMSGRTRVGEKLLISAAVEGG
                     ELVLEYRYSGLHYGEETMRKLGEAYKKNLGAIIGHCGQQDREVYTPSDYGLGGEVSYK
                     ELDRFLSDKATRITGLYRLSGLQEGMLFHGLYDGKGGAYLEQFSCELKGVDEAVFAGS
                     WSILLRRHSVLRSAFYYDAFRLPVQCVYGEVQLPLTVLDYRELGEAEQAAAVRAYEEL
                     DHEKGFDFREPPLLRVGLLRLSDERYRMVWTHHHILFDGWSRQILVEEFLAAYELLSS
                     GGILPGSEDDRYEDYIRYIEQRDKTLEEEYWRGCLEEVTTGTLLPFIRSGADRNKGDG
                     QYKEEMLVLDQEVAESIGAYAQRLKVTVNTVMQGAWAYLVHRYTGNREIIYGVTVAGR
                     PEDLAGMERRVGLYINTLPLCSRLEEGQETGAWLMAMQEGQLRSREYQYTPLSEIQRW
                     TGIRGELFDSLLVFENYPVSKVVSARSWRLEVGDLQIRQQTNYPLTLTVSIGEEIGIG
                     FTYNTGLLTLAEVRRIQAHFGHVLRQIIASAQLKIGDIALVTPAERQQLLSGFNNTVV
                     AYAGEKTVVDLFRQQAVRTPEKVAVVFGAEMLTYGELEGRSNQLARYLRELGVEEGGL
                     VPICVERSLEMVIGILGILKAGGAYVPLDPAYPADRLQYMLEDTAARVIVSSRACRGL
                     LPEKDGLVVVRPDEQQEEISRRSGEVILLNRKKGHLTYVIYTSGSTGRPKGVSMPEEG
                     LVNLLLWHEESQGHRADKRVLQFASINFDASFQELFSALCFGGRVYLIDEQSRKDAGE
                     LLKAMIAYGIHHLFIPYVVLKNISEHAREMELYPGELEAIFTAGEQLRLSEDIRVLCD
                     RTGAKLFNYYGPSEAHVVTAYEVRASDYGTRILPPIGKPIGNTSIYILDQRQDLCAVG
                     VVGEICIGGVQVASGYLHRPELTAEKFVANPYGKGRIYRTGDLGRWLEDGEIEFLGRR
                     DHQVKVRGNRVELGEVESVLGQCPGVRQCVVLDREDIHGYKLLVGYVVAEGTFSRENI
                     WWYMRSRLPDYMIPSVLIGLDSLPLTINGKVDKKALPQVDGDGLRKGQYVAPRTESEQ
                     ALTEIWAELLGIERIGIDDNFFELGGHSLLATRVVSAMRRLLGLEVAIRDLFIYPTIG
                     ELSAHLGAPVRGQVLPVIVAGQRPDRIPLSYSQERLWFIDQLEGGVQYHMPSVLRLRG
                     ALNVEAL"
BASE COUNT          989 a         1724 c          936 g         1029 t
ORIGIN      
        1 agcgcctcca cattcaacgc accccgcagc cgcagcaccg acggcatatg atactgcaca
       61 cccccctcca gctgatcgat gaaccacaac cgctcctggc tgtatgacaa cggtatccgg
      121 tcgggccgtt gcccagctac gatcaccggc agcacctgcc cgcgcaccgg ggcgcccaga
      181 tgggccgaca gctcgccgat agtggggtaa atgaacaggt ctctgatggc cacctccaat
      241 cccagcagcc ggcgcatcgc cgacaccacc cgcgtcgcca gcagcgaatg acctcccagc
      301 tcgaaaaaat tatcatcgat gccgatccgc tctatcccca gcagctccgc ccaaatttcc
      361 gtcaacgcct gctctgactc ggtccgcggc gccacatatt gccccttccg taaaccatca
      421 ccgtccacct gcggcaacgc ctttttgtcg acctttccgt tgatcgtcaa cggcaggctg
      481 tccagcccga tgagcacaga cggtatcata tagtcaggta agcggcttcg catgtaccac
      541 caaatatttt ccctgctgaa agtcccttcg gcaactacat aacccaccag cagcttatag
      601 ccgtgaatat cttccctatc caacaccaca cattgacgca cccccggaca ctgacccaat
      661 acactctcca cctcacctaa ctccacacgg ttaccccgaa ccttcacctg atgatcgcgc
      721 cgccctaaaa actcaatctc cccatcctcc agccagcggc ccaggtcacc cgtgcggtat
      781 atcctcccct ttccatatgg gttggccaca aacttctccg ccgtcagttc gggccgatgc
      841 aaataccctg atgctacctg gacaccccct atacagatct ctccaactac tcccaccgca
      901 cacaagtcct gccgttgatc taagatatag atcgacgtat tgccgatcgg tttgccgatc
      961 ggcggtaaga tacgcgtgcc gtagtccgat gccctcacct cataagccgt caccacatgc
     1021 gcctcggatg gcccgtaata gttaaaaagc ttcgcaccgg tcctgtcgca aaggacccgg
     1081 atgtcctcac ttaatctcaa ttgctcgccc gccgtaaaga tcgcttccag ctcacccgga
     1141 tatagttcca tctcccgcgc atgctcactt atattcttca acaccacata gggaatgaat
     1201 aaatgatgta tcccatacgc tatcatcgct ttcaacaatt ctcccgcatc cttgcgggac
     1261 tgctcatcga tgagatacac ccgcccgcca aaacatagcg cggaaaacag ctcctggaaa
     1321 cttgcatcga aattgatact ggcaaactga agcacccgct tgtcggcccg gtgtccctgg
     1381 ctttcctcgt gccacaagag taaattgacc aatccctctt ccggcatcga cactcccttg
     1441 ggacggcccg tcgaacccga ggtatagatc acataggtta aatgaccttt ctttctgttc
     1501 agcaaaatga cctcccccga cctccggctg atttcttcct gctgctcatc cggccgtaca
     1561 acgacaagtc catctttttc cggcaatagt ccccggcacg ccctgctgct cacgatcacc
     1621 cgggcagcgg tatcttccag catatactgc aaccggtcag ccggataggc cggatccaac
     1681 ggcacatatg ctccaccggc tttcaatatc ccaagaatcc caatgaccat ctcaaggctc
     1741 ctttccacac aaattggcac cagccctccc tcttcaaccc ccagctctct taagtaacgg
     1801 gccagttggt tgctccgccc ttccagttcc ccataggtca gcatctctgc tccaaacacc
     1861 accgccacct tctccggcgt ccgcacggcc tgctgccgga aaagatctac caccgtcttt
     1921 tcgccggcat acgccaccac cgtgttattg aaccccgaca acagctgctg ccgctctgca
     1981 ggggtaacca gcgctatatc tcctatcttc agctgcgctg atgcgatgat ctggcggagc
     2041 acatgcccga aatgagcctg tattcgccgc acctccgcca gtgtcagcag tcccgtatta
     2101 taagtaaacc cgatgcctat ctcctcccct atgcttaccg taagggtcag gggatagttg
     2161 gtttgttgcc ggatttgcag atcgcccact tccaaacgcc agctccgcgc ggaaaccacc
     2221 ttgctgaccg ggtagttctc aaaaaccagc aaactgtcga acagctcgcc ccgaattccc
     2281 gtccagcgct gtatctcgct caacggggta tattgatatt ccctgctcct cagctgcccc
     2341 tcctgcatcg ccatcagcca tgcccccgtc tcctgccctt cctccagtct tgagcacaac
     2401 ggcagcgtgt tgatatataa tcctacccgg cgctccatgc ccgccagatc ctccggccgg
     2461 cctgctaccg ttaccccata aatgatctcc cggtttcccg tgtaccgatg aaccaaatag
     2521 gcccatgctc cctgcatcac cgtattcacc gtcaccttta atcgctgcgc atatgccccg
     2581 atggattccg ccacttcctg gtcgagcacc agcatttctt ccttgtattg gccatctcct
     2641 ttgttgcggt cggctcccga acgaataaaa ggcaacaggg tgcccgttgt tacttcttcc
     2701 aggcagcccc gccagtattc ctcctccaac gtcttatctc tctgctcgat ataccggata
     2761 tagtcttcat accggtcgtc ttcactcccc ggcaatatac cacccgatga caacagctca
     2821 tacgccgcca aaaattcttc caccagtatt tgccgcgacc acccgtcgaa aagaatatgg
     2881 tgatgcgtcc ataccattcg gtaacgctca tcactcaacc gcaacagccc caccctcagg
     2941 agtgggggct cccggaaatc aaaacccttc tcgtggtcaa gctcttcata ggccctcacc
     3001 gccgccgcct gctcagcttc tcccaactcc ctgtagtcca gcaccgtcaa cggcagctgc
     3061 acctccccgt atacacactg tacaggcaga cggaacgcat cgtaataaaa cgcgctacga
     3121 agcacggaat gccggcgcaa aaggatcgac cagctgcccg caaataccgc ttcgtccacc
     3181 cctttcaatt cacagctaaa ctgctcgaga taagctccgc ccttgccgtc atataagccg
     3241 tggaacaaca tcccttcctg taacccgcta agccggtaaa gcccggttat tcgggtagcc
     3301 ttatccgata gaaatcgatc cagctcttta tagcttacct ctccgcctaa cccataatcg
     3361 gatggcgtat acacctctct atcctgctgc ccgcaatgcc cgatgatcgc cccgaggttc
     3421 tttttgtacg cttcccctaa tttccgcatc gtctcctccc catagtgcaa cccgctgtaa
     3481 cgatactcca gcaccagctc cccgccctct accgcagcgc tgatcaacag cttttcccct
     3541 acccgcgtgc ggccgctcat gctttctccc gccggacccc caacatcccg cagccacctg
     3601 ttattcctgg ttgcattatc caattgcccg aggtaattga accggatatc ccaggccgtc
     3661 tgctcatacc ccagcacacc atatccaatc cccttatcgg gtatccgccg caattgttcc
     3721 tttatcgcct tcaagagctc gcctgctgcc aaaccccgct ccacctccag cgccacggga
     3781 tacaggctcg taaaccaacc taccgtgcgg ctcacatcca ctccttcgaa taatccctcc
     3841 tcccggccat gtccctccaa cccgatggtt atcttctcac gactttgcca gtcgcagata
     3901 gtctgcgcca gcgccgccaa cagcacatca ttgatttccg tatgatagac ccggggcagc
     3961 tcctgcagca accgccttgt ctgctcctta tctaaccgca ccctgtaact gcccatatcc
     4021 tcgattcgca cctcccgggc gccgtcgctc cactccacct ccagcgcccg gtagcttccc
     4081 aacacctctt cccaatactt tcgctgttgc tggacgcgac ggctgcttcc ataggccacc
     4141 agccgctcgc gccactcccg gtacgaactg gtctttcttc ccggttcggc ggcgcccttc
     4201 gccaaaagct cttccaggtc ctccaggagg atccgccatg acaccccgtc caccgcaaga
     4261 tgatggatca cgatcagtaa cctgtcatcc tgctcccagt ccggcgtccg catccacacc
     4321 acccgcacga gctccccttt cccgatgtcc aggctccgct ggtagctgtc ggcccggcgc
     4381 ccgattgcct ggtcaagctc tcccgccgcg gcattccgca gatcttcctg gtccagccgc
     4441 cccatatatg tcccgtattc ctgctcccaa ccctcatccc cgcgatgata cacgaaccgc
     4501 agcccgtcat gccggcgcag cagcatcttc actgcatcct caagcacttc cccttttatg
     4561 ttcttgtcca cccccatcag cacccgctga ttataatagg atacatcatc ctgttcccgg
     4621 ctaaaatacc agcgctggat gggcattagc ccgcatcgtc ccgtcagcaa cccctgct
//
LOCUS       DATGFG010000622         4989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1105891, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000622 DATGFG010000000
VERSION     DATGFG010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105891"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1330
                     /gene="sucC"
                     /locus_tag="VKQ52_11615"
     CDS             119..1330
                     /gene="sucC"
                     /locus_tag="VKQ52_11615"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HLZ87886.1"
                     /translation="MNLHEYQAKELLKKYNVPVQEGIPVDNAGAAEEAYKNLKVQFGN
                     NFAVVKAQIHAGGRGKGKIEGTEQRGVAVGKSAEDIKQIASNILGGTLVTIQTGPAGK
                     KVNKVLIAQDVYYPGANPVKEFYLSVLLDRARGQNVIMYSTEGGMDIEEVAHKTPDRI
                     FKEWVHPGGGLQAFQARKIAFNLGLSGEAFKNCVKFVTNLYNAYVGLDCSMLEINPLF
                     KTSDEKIIAVDCKMNLDDNALMRHPDLEALRDISEEDPTEVEAGKYNLNFVKLDGNVG
                     CMVNGAGLAMATMDMIKLSGGEPANFLDVGGTANATTVEAGFRIILKDPKVKAILINI
                     FGGIVRCDRVAQGVIDAYKSIGDIKVPIIVRLQGTNAEEAQQLIAQSGLKVQSAILLS
                     EAAALVNKAVS"
     gene            1330..2154
                     /locus_tag="VKQ52_11620"
     CDS             1330..2154
                     /locus_tag="VKQ52_11620"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HLZ87887.1"
                     /translation="MGLTTLQQIEETVKAIRAVCATAPKVGIVLGSGLGNLSKEIRVE
                     RELSYADIPHFPVSTVEGHHGKLIFGTLNDRPVVTLSGRFHYYEGYTPQQVIYPIRVL
                     KFLGVETLLLSNAAGGMNKAFHVGDLMIINDHISLFTVNPLLGRNEPLLGPRFPDMSE
                     PYSRELIRKAKAIASGLGISVHEGVYAGVTGPTFETRAEYKLLHIAGGDAVGMSTVQE
                     VIAARHLGMSVFAMSVITDLGIREEDNIITHEEVLQAAAAAEPKLTALFKGLIAAL"
     gene            2210..4267
                     /locus_tag="VKQ52_11625"
     CDS             2210..4267
                     /locus_tag="VKQ52_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HLZ87888.1"
                     /translation="MNRRRIIIAGVFLVMLLSGSGLLAQNPLGRFGNIGSGGGGGKDS
                     LQHRKQDTITINFRYLDSSKLYRFDSSILDLGRKLPLRSTWINVGNIGNAARSLIFTP
                     RMQSGWDPGLHAYDLYTFTVDETRFFHTTKPYTELGFFLASKAEQFIDVFHTRNIRPN
                     WNYSFEYRLINAPGTFQNQNTNHNNYRLTSWYQSRNKRYQNFFILVASKLGSSENGGI
                     QNPRDLDSVQYSNQTTVPVKLGQNLLQSTGNPFSSSITTGTKYSSATYLMRQQYDLGQ
                     KDSIVTDSSVIPLFYPRVRLEHTMAYSTYSYRFFDLYYPTSYTVDSAYYAQNLGLVNV
                     HPTDSFWRQDNWRNFSNDFSIYQFPDSKNPQQFIKLGATLELLTGNFDTAQLATGVLY
                     TAHKLNNQNVFAHGEYRNKTRNQKWDIEAAGRLYLNGLNAGDYNAYISLKRLISRQVG
                     YFQAGFENANRTPGYVFNGVSSFNLDTASQHKGFSKENTTHLFASLEQPQHQLLLSAS
                     YYLMTNYSYFYKYYKEAQASVFNILQVNFRKQFTLYRHWKWRTNTTIQQVAGSSPVHV
                     PLIVSANQVGYDGNFGLKNLVLSFGTEIRYVSSYKADGYSPAVGQFFSQDSTIRQKAP
                     DVNLYLHLRIRSFTGFLRAENVNALAFSPHGFGFYHNNFVAPYYPSPGMIIRFGFFWG
                     FIN"
     gene            complement(4297..>4989)
                     /locus_tag="VKQ52_11630"
     CDS             complement(4297..>4989)
                     /locus_tag="VKQ52_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLZ87889.1"
                     /translation="VKPTEKYTYGYKKTTILVALLNAAILLLSLGAIGYEAVHRLMEP
                     HPLPGKTISIVAAIGIAINAITALLFFRSKENDLNIRSAFLHLLSDAIVSAALVIGGI
                     IIFLTQLYWIDAVLSLLVAVIILFSTWQLLRDSLRLSLDGVPGGIEIKKVRETIAKMD
                     GVRDFHHIHIWAISTTENALTAHLVVDRNCTMEDAEQLKHRIKHELLHLNIQHATLEV
                     EMENAPCDEPDC"
BASE COUNT         1116 a         1293 c         1454 g         1126 t
ORIGIN      
        1 gccggagtgc ggcctttgcg gtgaattatc ttattttatt atgcgtaagc gcgtcagcga
       61 gggttttttt tccggtttag gtgattctgc cctattttcg cactaaattt tttcctccat
      121 gaacttgcat gaatatcagg ccaaagaact actgaaaaag tataatgtac ccgtacagga
      181 aggcatcccg gtagataatg cgggggctgc cgaagaagca tacaaaaatc ttaaagtaca
      241 gtttgggaac aattttgcag tagtgaaggc tcagatccat gccggtggac gtggtaaagg
      301 aaagatcgaa ggtacggagc agcgcggtgt tgccgtgggc aagagtgccg aggacatcaa
      361 gcagatagcc tccaacatcc tggggggtac gctggtgacg atccagaccg gtcctgccgg
      421 aaagaaggtg aacaaggtgc tgatcgccca ggacgtttat tatcccggcg ccaatcccgt
      481 gaaggaattc tacctgtcgg tgctgctcga ccgcgccagg ggccagaatg tgatcatgta
      541 cagtacggaa ggcggtatgg atatcgagga agtggcgcac aagaccccgg acaggatctt
      601 caaggagtgg gtgcatccgg gtggcggcct tcaggctttc caggcccgca agatcgcctt
      661 taacctcggg ctgagcggcg aagcgttcaa gaactgtgtg aaattcgtta ccaatcttta
      721 taatgcctat gtagggctcg attgttcgat gctcgagatc aatcccctgt tcaagaccag
      781 cgacgagaag atcattgccg tcgactgcaa gatgaacctg gatgacaatg ccctgatgcg
      841 gcatccggac cttgaggcgc tgcgggacat cagcgaggaa gatcccacgg aggtcgaggc
      901 cggcaaatac aacctcaact ttgtcaaact ggatggtaac gtaggttgta tggtcaatgg
      961 cgccggtctt gccatggcga ccatggatat gatcaaactc agcggcggcg agcctgccaa
     1021 cttcctggac gtgggtggta cggccaatgc cacgacggtg gaagccggtt tccggatcat
     1081 tctcaaggac cccaaggtaa aagccatcct gatcaatatt ttcggtggta tcgtccgttg
     1141 cgaccgggta gcccaggggg ttatcgatgc gtacaagtcc atcggcgaca tcaaggttcc
     1201 catcatcgtg cggctgcaag gcaccaatgc cgaagaggcc cagcaattga tcgcccagag
     1261 cggcttaaag gttcagtccg ccatcctgct tagtgaagcg gcggcgctgg ttaataaagc
     1321 cgtttcctga tggggttgac cacattacaa cagatagaag agaccgtgaa ggcgatccgg
     1381 gcggtgtgcg ccacggcgcc gaaggtaggt atcgtgctgg gtagcggtct tggtaatctt
     1441 tcaaaggaga tccgcgtcga gcgggagctc tcctatgccg acatcccgca ttttcccgtt
     1501 tctacagtag aaggccatca tggaaagctc atcttcggga cccttaacga ccgtcccgtc
     1561 gtaacgctct ccggccgctt ccattactat gaggggtata ctccgcagca ggtgatctat
     1621 cccatccggg tgttgaaatt tctgggggta gagaccctcc tcctttccaa tgccgccggc
     1681 ggaatgaaca aggcctttca tgtcggcgac ctgatgatca tcaacgatca tatcagcctt
     1741 tttacggtga atcccttatt gggccggaac gaaccgttgc tgggaccccg ctttcccgat
     1801 atgagcgagc cctatagccg ggagctgatc cggaaggcga aggctatcgc ttcaggatta
     1861 gggatctccg tgcatgaagg agtgtatgcg ggggttaccg ggccgacttt cgagacccgt
     1921 gccgagtaca agctcctcca catcgccggt ggtgacgccg tcgggatgag cacggtgcag
     1981 gaggtgatcg cggcccgtca cctggggatg tccgtatttg cgatgagtgt gatcaccgat
     2041 ctcggtatcc gcgaagagga caatatcatc acccatgagg aggtattgca ggcggcggca
     2101 gccgcagaac ccaagctgac ggcgctgttc aaagggctga tcgcggcgtt gtaaggatta
     2161 acttttctta tgcgacttaa tgagtaaatt gtccggaatt aatgcagcag tgaaccgacg
     2221 tcgaattata atcgcaggag tattcctggt gatgctgtta tcgggatcag gtctcctggc
     2281 gcaaaatccg ctgggccgtt ttggcaatat cggttccggg ggaggcggcg gaaaagacag
     2341 cctgcagcat cgcaaacagg acacgatcac catcaatttc cgttatctcg attccagtaa
     2401 gctgtaccgg ttcgactcct ccatcctcga cctggggcga aagctgccgt tgcgatccac
     2461 ctggatcaac gtgggcaata tcgggaatgc ggcccgcagc ctgatcttta cgccaaggat
     2521 gcaatcgggc tgggatccgg gcctgcatgc ctatgatctc tataccttca cggtcgacga
     2581 gacgcggttc tttcatacga caaagccgta taccgaactg ggttttttcc ttgccagcaa
     2641 ggcggagcaa ttcatcgatg tctttcacac gcggaatatc cggcccaact ggaattactc
     2701 gttcgagtac cggttgatca atgcgcccgg aaccttccag aaccagaata ccaaccataa
     2761 caactaccgg ctcacgtcct ggtatcagtc gaggaacaag cggtatcaga atttttttat
     2821 actggtagcg agcaaactgg ggtcttccga gaatggcggg atccagaatc caagggacct
     2881 cgactccgtg cagtacagca accagacgac cgtcccggtg aagctggggc agaacctgct
     2941 gcaatccacc ggtaaccctt tctcctcatc gatcacgacg ggaacgaagt atagctcggc
     3001 cacctacctg atgcgccagc aatacgatct ggggcagaag gattcgatcg tgacggactc
     3061 ttcggtgatc cccctgttct acccgcgggt gcgcctggag catacgatgg cgtacagcac
     3121 gtacagctac cggttcttcg atctttatta tccgacctcc tataccgtgg attccgctta
     3181 ttatgcccaa aacctggggc tggtgaatgt ccatcccacg gacagtttct ggcggcagga
     3241 caactggcgg aatttctcca atgactttag catctaccag ttccccgact cgaagaaccc
     3301 gcagcagttt atcaagctgg gggctacgct cgaattgctg accggcaatt tcgatacggc
     3361 gcaactggcg acgggggtat tgtatacggc ccataagctc aataaccaga atgtctttgc
     3421 ccatggagaa tatcggaaca agacccggaa ccagaaatgg gatatcgagg cggcgggccg
     3481 gctttacctc aacgggctga acgccgggga ttacaatgca tatatcagtc tgaagcggct
     3541 gatcagccgg caggtggggt atttccaggc agggttcgag aatgcgaacc ggacgcccgg
     3601 ctatgtcttt aacggggtca gcagcttcaa tctcgatacg gcttcgcagc acaagggctt
     3661 tagcaaggag aacacgaccc atctttttgc ctcgctcgag cagccgcagc atcagctgct
     3721 gctgagcgcc tcgtattacc tgatgaccaa ttactcctat ttttataaat actacaagga
     3781 ggcccaggcg tccgtattca atatcctgca ggtaaatttc cggaaacagt tcaccctcta
     3841 ccggcattgg aaatggcgca ccaatacgac gatccagcag gtggccggtt catcgccggt
     3901 gcatgttccg ctgatcgttt cggccaacca ggtggggtat gatgggaatt tcgggttgaa
     3961 gaacctggtc ctcagttttg gaacggagat ccgctatgtc agttcttata aagccgatgg
     4021 gtattcgccg gcggtggggc agttcttcag ccaggattcg accattcgcc agaaggcgcc
     4081 ggatgtcaac ctgtacctgc atctgcggat aaggagcttt accggctttc tgcgtgccga
     4141 gaacgtcaat gcgctggcct tcagcccgca tgggttcggc ttttatcata ataacttcgt
     4201 agcgccctat tatccctcgc cggggatgat catccgtttt ggatttttct ggggatttat
     4261 caattaggcc acaggcctgg cggccgaagg agcgaactag caatccggtt catcgcaggg
     4321 ggcgttttcc atctctactt cgagggtggc gtgctggatg ttgaggtgca gcagttcatg
     4381 tttgatcctg tgcttcagct gctcggcgtc ttccatggtg cagttgcggt cgacgacgag
     4441 atgggcggtg agggcgtttt ccgtcgtgct gatggcccag atatggatat gatggaagtc
     4501 tcttacccca tccattttcg cgatggtctc ccgcactttt ttgatctcga tgcctccggg
     4561 tacgccgtcg agggacaggc gcaggctatc gcgcaggagc tgccaggtgc tgaagaggat
     4621 gatgacggcg acgagcaaac tcaggacggc gtcgatccag tacagctggg tcaggaaaat
     4681 gatgatgccg ccgatgacga gggcggcgga tacgatggcg tcggacagca ggtggagaaa
     4741 ggcgctgcgg atattcaggt cgttctcctt gctgcggaag aagagcaggg cggtgatggc
     4801 attgatcgcg atgccgatgg ccgcgacgat ggagatggtc ttgcccggca gggggtgggg
     4861 ttccatgagc cggtgcaccg cctcgtaccc gatggcgccc agggacagca gcaggatcgc
     4921 ggcgttgagc agggcgacga ggatcgtcgt ctttttatag ccgtaggtat atttttcggt
     4981 gggctttac
//
LOCUS       DATGFG010000623         9996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_514586, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000623 DATGFG010000000
VERSION     DATGFG010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514586"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..575)
                     /locus_tag="VKQ52_11635"
     CDS             complement(15..575)
                     /locus_tag="VKQ52_11635"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLZ87890.1"
                     /translation="MNTLSDNALMLKVRSGDLDMMGLLFQRYHRPLYGFLFHMTQERE
                     ASEDMMQNVFYRMLRSRHTFTGEGEFRTWMYHLARNVLKDHFRQTRRKGNHYEIGEYE
                     ERLSAGVAADEQVERKLEIKALERALGHLSPESREVLVLSRFQELKYAEIAQVLEISV
                     GAVKVRVHRAIHQLKERYWQSETENK"
     gene            722..1180
                     /locus_tag="VKQ52_11640"
     CDS             722..1180
                     /locus_tag="VKQ52_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HLZ87891.1"
                     /translation="MITSTVSQLHWYRIISPNFDKAIQYILSMDLTALETGKYPIDGD
                     DVFAIVNEYTTKPASECDPESHRDYADIQVVVAGVERFGYAPLVDQSETTPYDPEKDV
                     AFYTIAAEDLSYITLHAGEFIIFFPSDIHQPELFTQQPDLVRKIVLKVAL"
     gene            1185..1652
                     /locus_tag="VKQ52_11645"
     CDS             1185..1652
                     /locus_tag="VKQ52_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87892.1"
                     /translation="MDQSEKLAFKATLKKWALDLLGQRIATTNEAMRQAQEAANSEEK
                     SSVGDKYETGRAMGHLQKDMHARQLAELVRELAALHAVNTDQLYQEARTGALLQTDKI
                     DFFISIGLGRQHIDGRTIVFLSPLAPLARTLQDKKAGDSILFDRETMPIRRIF"
     gene            1731..3692
                     /locus_tag="VKQ52_11650"
     CDS             1731..3692
                     /locus_tag="VKQ52_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HLZ87893.1"
                     /translation="MRTTRLFLLAGLTSLASLAALTVFAQDPGSEPVALIPAPVSQTV
                     NAGHFELPKTIVIEAQDQRDLDQGLQDLKTRLSLPTGYTVTITRQPTPDATIRLVLNK
                     SAEATLGAEGYYLSITPQAVLIRANQAAGLFYGIQTLLQLLPKEIESRQVVNRHTWPA
                     PCITITDYPRFGWRGLMFDVSRHFFTKQEVKDFIDEMVKYKFNLLHMHLTDDEGWRVE
                     IKSLPRLTEVGAWSAKRVGTFGMFADPAPDEKRDYGGFYTQDDIRDLVRYAKARFVNI
                     LPEVDVPGHSLAAIVSYPELSCTEGADKYHVRSGEKIMNWYKGGFDALYDNTLCPANE
                     KIYPFLDKVFTELAALFPFHYIHVGGDECAKNFWEKSPAVKALMQKEHLQTMEQVQSY
                     FEKRVEKIVESKGKKVIGWDEILEGGLAPNAAVMSWRGVKGGIAAAKMGHQVVMSPTT
                     FFYLDYMQGDATVEPPVYATLRLRTTYSYEPVPDSVDPKLIIGSQANLWTEQVYNTRH
                     MQYMVWPRSMAVAEDLWTPKEKKNWDDFAGRVEANFARLDVEQVKYARSMFDAIITPK
                     KDISANDSVTVDLATEVSGLDIYYSFDNSNPDNFYPKYTGTLTIPKDASWLKVITYRD
                     GKPIGHQLNISVLALQQRVGLREAPKEVD"
     gene            3702..4397
                     /locus_tag="VKQ52_11655"
     CDS             3702..4397
                     /locus_tag="VKQ52_11655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ87894.1"
                     /translation="MKDILGQALTDYYRQGHAGKLWVHRSNGKKEEMPVEIYFRDMTS
                     MPELEWRALQYCRGRVLDIGAGAGSHALALQRLGLDVTALDISPLGTTLMEQRGVKKV
                     IHQDFFLLKTGAYDTLLLLMNGIGLAGDLDGLSLFFTRARRLLRPGGQLLFDSSDIAY
                     LYSGKPPKGPDYYGIIDYQYEYKRQRSDWFRWLFVDRKTLSAVAAKEGWSTEILLEDR
                     HGQYLARCRPVSS"
     gene            complement(4387..5433)
                     /locus_tag="VKQ52_11660"
     CDS             complement(4387..5433)
                     /locus_tag="VKQ52_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87895.1"
                     /translation="MLAWFAPGLVSAMLGLYVIREAQVTNVTVDDYALTQRTVFSTRS
                     IPLKELEGYREGGKDSFRLIPKQGRSLQLTTAIADRRELIEWVKDNYPDIDKREREAE
                     EATLLRNERFGATQSEREATLVKARQFTRIANIGGMALGLWFVFYPQPFDLSLTLIFL
                     APLVALVMIRRFRGLIRFYSVKSSPYPSLGMMIGFTAGGGAVRAGMAYNLYEFPSSAW
                     LLLLAGAAGMLLLIFLLAREAFTGEKRKSLVIIGVAVAALAYSYGLLVNTNCHYDRST
                     ARVLPVAVTGKRASHGKSTTYYLQLAPWGRFPEGREVDVSKSFYNNIQPRDTVLVLLN
                     QGRWDIPWFRLVSY"
     gene            complement(5643..5948)
                     /locus_tag="VKQ52_11665"
     CDS             complement(5643..5948)
                     /locus_tag="VKQ52_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87896.1"
                     /translation="MKKTACIQLVLITAALAACNRPMYQQEYYSPAYYPGDLPDSTNS
                     CPIDYSPFPPDYYNWWYGFRPYGTVYVDPYVNVYYRHRHRETVRRSGFGGKISAVSS"
     gene            6175..7641
                     /locus_tag="VKQ52_11670"
     CDS             6175..7641
                     /locus_tag="VKQ52_11670"
                     /inference="COORDINATES: protein motif:HMM:NF023283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HLZ87897.1"
                     /translation="MPTAELSYLLETSPSVQIIRMRNAHWVIPFLFRAFKSENILSLP
                     EQTLINALAEELRNHSEDIEDLEEARIEFGEDEESRARKYLLNWVQRRLLQDFPDTDA
                     VTQYQLSAHTEKLFQWLQSLEKRQFVGTESRFRFLFQTLREMVEYTEDDAAKRLEELK
                     NRKAEIDKEIKKIEMGYKPEVYTNAQLRERLEWFTRLSHELLSDFREVEDNFKTIHRH
                     IVEQHTRAEINKGAIVGYAFEAYDALRKSDQGRSFYAFWDFLVSRAGQEEWRELTDQL
                     LQMLQDRNIESDGEFVQDIKSLLLQQGRNVYESNDRMAEKLSRIITEKEIARHRRLRQ
                     QIGQIKELVFKFMDDETPSCGIRLDTPAPIRMNMERRLNLVEKQAAGVVKQPANAREK
                     VEDLERFGRLLNTTHVDRKKLWQKVETVLEQKTTATLAEIIGITGLEHGVAEVVGYFS
                     FLREKAAKVQSMQEITELIPLDEEKTRFIEVPYLLFSR"
     gene            7693..8283
                     /locus_tag="VKQ52_11675"
     CDS             7693..8283
                     /locus_tag="VKQ52_11675"
                     /inference="COORDINATES: protein motif:HMM:NF025205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HLZ87898.1"
                     /translation="MNTDKQNILPYTPVFIKLLKSPVEYLEKTAWEQLLQYQGELTRF
                     LQQLGLVLVLEKEDGYAYLEQARLDEEENVIGWVRRIQLGYEESVLLVLLRDMMAEFE
                     VGEAGARELIRKRREIKEYAELFFRENASRVKFIRDLDRLIDRVEELDFLEKVEQHEL
                     QDEQKFRVKKIIKARVDNEILENFKQQLSEHAVGRI"
     gene            8264..>9996
                     /locus_tag="VKQ52_11680"
     CDS             8264..>9996
                     /locus_tag="VKQ52_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87899.1"
                     /translation="MQLGVFSTDENKSGFRLQYMELFNWGTFDEHVWKITPGGETSLL
                     TGANGSGKTTFVDALLTLIVPEKKYRFYNQSSGSEKKGDRTEESYVLGGYGSVHSDNG
                     LSSKTLYLRDNKEQAYSILLAHFSNEAEQSVTLFQVRWFSGHEMRRVFAIAHKPLHIE
                     DDFKPFDLGGAWRRRIDQQYNKGSRKQVEWFDAASRYAQRMVEVLGMQSMQALSLFNQ
                     TMGIKVLGDLNEFIRGNMLEPRNMEQEFQELKKHLSTLLDAQRNIEKTGEQVRLLHPL
                     KKHFLAYKEQTDLGAAARQELDTARIWKNYTKYHLLDEYIRSQEKDAGNLRRDSEEAK
                     QKITTLLEEERQTLNQLEQNKAGQRLQQLQKEREGLEQQVKETEAALKKFADWCALLH
                     LETSPADEAAYQRIKKEAERTDKKLATEIRNNNEDLYEAQDRQKKATEEKTKIEGELN
                     TLLQSRNNIPAHLVALRRDLCAELKIDDQELPFAGELIQVRNEEMDWQPALEKLLNSL
                     ALRLLVPEKHYRKVSRYVNNTQLKSRLVYHLIKDIALQQHADPGTVWHKLEFQPGHPL
                     SSWVSQQVIQQ"
BASE COUNT         2550 a         2960 c         2606 g         1880 t
ORIGIN      
        1 atccacattg catactattt attttcggtt tccgattgcc aatagcgttc tttcaactga
       61 tggatggccc ggtgtacccg caccttcacg gcgccgacgc tgatctccaa cacctgggct
      121 atctccgcat atttcagttc ctggaagcgg ctcagcacca gcacctcccg gctttccggg
      181 gacaggtgac caagcgccct ttccagggcc tttatctcca gcttccgctc aacctgctca
      241 tccgccgcca ccccggccga aagccgctcc tcatactcgc cgatctcgta atgattccct
      301 tttcgccgcg tctgcctgaa atgatctttc agaacattac gcgccaaatg gtacatccag
      361 gttcggaact ccccctctcc cgtgaaggta tgacgcgacc ggagcatccg gtagaacaca
      421 ttctgcatca tatcctcgct tgcctcccgc tcctgcgtca tatggaagag aaaaccatat
      481 agcggccggt ggtatcgctg gaacagcaag cccatcatat ccagatcgcc ggatcttacc
      541 ttcagcataa gcgcgttatc cgagagtgtg ttcaagcagc agggttattc tgataaaaga
      601 tagaagggaa actttatccc gctaaaaagg ttacccaaaa tgccggattt attttttctc
      661 ccgcaaaagg gctaacttcc gccggcaaaa aaaccaacca caagcaaaca agtcatcgcc
      721 catgatcacc tcgacagtat cgcagttaca ttggtacagg atcatcagcc cgaattttga
      781 caaagccatc caatacattc tttccatgga tcttaccgcc ctggaaacag ggaagtatcc
      841 catcgacggc gacgatgtct tcgcaatagt caatgaatat acgacaaagc ccgcgtcgga
      901 atgcgacccc gagagccatc gcgactacgc ggatatccag gtcgtggtcg ccggggtaga
      961 gcggtttggc tatgccccgc tggtcgatca gtccgaaacc accccctacg acccggagaa
     1021 agacgtcgcc ttctatacca tcgccgccga agacctcagc tatattaccc tgcacgccgg
     1081 ggaattcatc attttcttcc cctccgacat ccatcagccg gagcttttta cccagcagcc
     1141 cgacctcgta agaaagatcg tcttaaaagt cgccctgtaa acccatggat cagtcggaaa
     1201 aactcgcatt caaagccacg ctgaaaaaat gggccctcga cctcctcgga caacgcatcg
     1261 ccaccacgaa tgaggccatg cgccaggccc aggaggcggc caacagcgaa gagaaaagct
     1321 cggttggcga caagtacgag accggccggg ccatgggcca tctgcaaaaa gatatgcatg
     1381 cgcgtcaatt ggcggaactg gtccgggagc tggccgccct ccacgccgtc aacaccgatc
     1441 aactctacca ggaagcaagg acaggcgccc tgctccaaac cgacaagatc gattttttta
     1501 tcagcatcgg tctcggcaga cagcacatcg acggcaggac catcgttttc ctctcgccac
     1561 tggcgccgct ggcccgaacc ttgcaagaca aaaaagccgg cgacagcatc ctcttcgatc
     1621 gcgaaacgat gcccatccgg cggattttct gacaaaacat agtacattct tccaataaat
     1681 gttaccttga caaacagatc attcacaaac taaaacagtt gccaaccaag atgagaacga
     1741 cacgtctgtt tcttctcgcg ggcctcacaa gcctggcgag tcttgctgcc ttaaccgtct
     1801 tcgcccagga ccccggatcc gaacccgtcg cactgatccc cgcacccgta agccagaccg
     1861 tcaatgccgg acatttcgag ctcccaaaaa cgatcgttat cgaagcccag gaccagcgcg
     1921 atctcgacca gggcctccag gatctaaaaa cccggcttag cctgccgacc ggatataccg
     1981 tcaccatcac ccggcagccg acacccgacg ccaccatccg gctggtgctg aacaagtccg
     2041 ccgaggcgac tcttggcgcc gagggctact atctctccat taccccccag gccgttctta
     2101 tccgcgccaa ccaggccgcc ggccttttct atggcatcca aaccctgctc cagctgttgc
     2161 caaaagagat cgaaagccgg caggtggtga accggcatac ctggcccgcc ccctgcatca
     2221 ccatcaccga ctacccgcgc ttcggctggc ggggactgat gttcgacgtg tcccgtcatt
     2281 tctttaccaa acaggaggta aaggatttta tcgacgagat ggtaaaatac aagttcaacc
     2341 tgctgcacat gcacctcacc gatgacgagg gctggcgggt cgagataaag agtcttcccc
     2401 ggctcaccga agtaggcgcc tggagcgcaa aacgcgttgg aacctttgga atgtttgccg
     2461 accccgcccc ggatgagaaa cgcgactacg gcggcttcta tacccaggac gatatccgcg
     2521 atctggtccg gtacgcaaag gcccgcttcg tcaatatcct ccccgaggtc gatgttcccg
     2581 gccacagcct tgccgccatc gtttcctatc ccgaactctc ctgcacggaa ggcgccgaca
     2641 aataccatgt acgctccggc gaaaagatca tgaactggta taaaggcggt ttcgatgcgc
     2701 tctatgataa taccctctgc cccgccaacg aaaagatcta tcccttcctc gataaggtct
     2761 ttaccgaatt ggccgccctg ttccccttcc actatatcca tgtcggtggc gacgaatgcg
     2821 ccaagaactt ctgggaaaag agcccggccg tcaaggcgct gatgcagaaa gaacatctgc
     2881 agaccatgga acaggtgcag agctatttcg agaaacgcgt cgaaaagatc gtggagtcga
     2941 aaggcaagaa ggtcatcggc tgggacgaga tcctggaagg cggcctcgcc ccgaatgcgg
     3001 ccgtcatgag ctggcgcggg gtaaagggcg gtatcgccgc ggcaaagatg ggtcaccagg
     3061 tggtgatgag cccgaccacc ttcttctacc tggactacat gcagggagac gccaccgtcg
     3121 agccgccggt atatgccacg ctccggttaa gaacgaccta ttcctatgag cccgtacccg
     3181 acagcgtgga cccaaaactt attattggca gccaggctaa tctctggacg gaacaagtct
     3241 ataatacccg ccatatgcaa tacatggtct ggccccgtag catggccgtg gcggaagatc
     3301 tctggacccc aaaagagaaa aagaactggg atgatttcgc cggcagggtc gaagctaatt
     3361 tcgcccgcct ggatgtggaa caggtgaaat atgcccggtc gatgttcgat gccatcatca
     3421 ctccaaagaa agatatctcg gccaatgact cggttaccgt cgatctcgcc accgaagtga
     3481 gcggcctgga catctattat agttttgaca attccaatcc ggacaatttc tatccgaaat
     3541 ataccggcac actgaccatc cccaaggacg cttcctggtt aaaggtgatc acctaccggg
     3601 atggcaagcc catcggccat cagctcaata tctccgtcct ggccctgcaa cagcgggtcg
     3661 gcctgagaga agccccgaag gaggtggatt aagccgacga catgaaagat attcttggcc
     3721 aggccctgac ggactattac cgtcaggggc acgccggcaa gctctgggta catcgcagca
     3781 acgggaagaa agaagaaatg ccggtggaga tctatttccg cgacatgacc agcatgcccg
     3841 aactggaatg gagagccttg caatattgcc ggggacgggt cctggatatc ggggcgggag
     3901 cggggagtca tgctctcgcc ctgcagcggc tcggcctgga tgtaacggcc ctggatatct
     3961 ccccgctcgg tacgacgctc atggagcagc gaggggtgaa aaaagtgatc caccaggatt
     4021 tctttttgct gaagacaggc gcctatgaca ccctcctgct gctgatgaac ggcatcggac
     4081 tggccggcga cctcgacggt ttgagcctct tctttacccg cgcccgccgg ctgctgcgtc
     4141 ccgggggcca gttgcttttc gattcctcgg acatagctta tctctactcc ggcaagccgc
     4201 caaaaggccc ggactactat ggcattatcg actatcaata cgaatacaaa cgccaacgct
     4261 cggactggtt ccgatggctg ttcgtcgatc ggaagacgct gtcggcagta gcggcaaagg
     4321 agggatggag caccgagatc ctgcttgaag accggcatgg tcaatatctc gcgcgatgcc
     4381 ggccggtcag tagctgacca accggaacca ggggatatcc catcttccct gattcagcag
     4441 gaccaatacc gtatcccggg gttgtatatt gttataaaaa gatttgctga catccacttc
     4501 tctcccttcg gggaatctcc cccagggcgc gagctgcaga tagtaggtcg tactcttgcc
     4561 atggctggcg cgtttgccgg tcactgccac cggcagcacc cgggccgtag atcgatcata
     4621 gtggcagttg gtattcacga gcagtccata gctataggcc agggccgcca ctgcgacccc
     4681 gatgatgaca agggattttc tcttctctcc ggtgaaggct tctcgagcca gcaaaaatat
     4741 aagcaaaagc atcccggccg ccccggccag cagtagcagc caggcggagg agggaaattc
     4801 atacaggtta tacgccatcc ccgcccgcac ggccccgccg ccggccgtaa aaccgatcat
     4861 catcccaaga gaagggtagg ggctgctttt cacagaatag aaacggatca gccccctgaa
     4921 acgccggatc ataaccagcg cgaccagcgg agcaagaaag ataagcgtca gggaaaggtc
     4981 aaagggctgg ggataaaaca cgaaccagag gccgagggcc atcccgccga tattggcgat
     5041 ccgggtaaat tgccgggctt tgacaagcgt cgcctcgcgt tcgctctgtg tggcgccaaa
     5101 ccgctcattt ctcaacaacg ttgcctcctc cgcttcccgc tcccgtttgt cgatatccgg
     5161 gtaattgtcc tttacccact cgatcagctc ccgccgatcg gcgatagccg tcgtcaattg
     5221 caaggaccgg ccctgtttgg gaatcagccg gaaactatcc ttgcctccct cccggtatcc
     5281 ttccaactcc ttaagcggaa tgctccgcgt ggagaaaacc gtccgctgcg tcagcgcgta
     5341 gtcatcgacc gtcacattcg tgacctgcgc ttcccggatg acataaaggc caagcatcgc
     5401 ggagacgagt ccaggcgcga accaggctag cattgggccg acagatccgg agcgggcggc
     5461 atgataaaaa aggaaaacgg cgcccgtaag gcaaaccggc aataagaggt agcaaaaggc
     5521 tttataagcc cggttcatgg ggtattcctt catggggttt cttgggctca gggttgtctt
     5581 gggctcatta aataaacaaa aaatccccgg cttctgcaac gacattaaca agcttaacaa
     5641 attcaggacg ataccgcact gatcttgccg ccaaatcctg acctccgcac ggtctcgcga
     5701 tgacggtgcc gataatatac attcacataa ggatcaacat ataccgttcc ataaggtctg
     5761 aatccatacc accagttgta atagtcgggc ggaaaggggg agtagtcgat agggcaggaa
     5821 ttggtggaat ccggcaggtc gccgggataa taagccgggg aataatactc ctgctggtac
     5881 atgggcctgt tacaggcggc aagggcagcc gtgatcagga caagctgaat gcaggcggtc
     5941 tttttcatga cggtatagcg ggcgttggtg ctgcataaat gtactcccca actccccgat
     6001 ccgccgttat ccgctggtta tgggctggtt attccttcgt tatcggctat tgtgtccgca
     6061 cccctgaaat tttccacgac aaggccgaaa ttatccccaa cgctggtttt ttttatcccc
     6121 accacggtct ggccctcaac atcgtatccg gtgaatcgat aatttcagcc cttgatgcca
     6181 acagcggaat tatcctatct actcgaaact tccccatccg tacagatcat ccggatgcgc
     6241 aacgcacact gggtgatccc cttcctgttt agagccttca aatcggagaa catcctttcc
     6301 ctgccggagc agaccctcat caatgccctc gccgaagagc ttcgcaacca ttccgaggac
     6361 atcgaagacc tggaagaagc ccggatcgaa ttcggcgaag acgaagagag ccgggcgcgt
     6421 aaatacctgc tcaactgggt ccagcgccgc ctgttacagg atttccccga caccgacgcc
     6481 gtcacccaat accagcttag cgcccatacc gagaagcttt ttcaatggct gcaaagcctg
     6541 gagaaacgcc agttcgtagg tacggaaagc cggtttcgct ttctcttcca gaccctgcgg
     6601 gaaatggtcg aatacaccga ggacgacgcc gcaaaacgac tggaagagct aaagaaccgg
     6661 aaagccgaga tcgacaagga gatcaaaaag atcgaaatgg gctataagcc cgaagtctat
     6721 accaacgcgc agctgcgcga acgcctggaa tggttcaccc gcctcagcca tgaactgctt
     6781 agcgatttcc gggaggtgga agacaatttc aagaccatcc accggcatat cgtcgagcag
     6841 cacacccgcg ccgagatcaa taaaggcgcc atcgtcgggt acgccttcga agcctatgac
     6901 gccctgcgca agagcgacca gggccggagc ttttatgcct tctgggactt cctcgtctcc
     6961 cgggcaggcc aggaggaatg gcgcgaactc accgatcagc tgctgcagat gctgcaggac
     7021 aggaacatcg aaagcgatgg agagttcgta caggatatca aatccctgct cctgcaacag
     7081 gggcgtaatg tatacgagtc caacgaccgg atggcggaga agctgagccg gatcatcacg
     7141 gagaaagaga tcgcgcgcca tcgccggctg cgtcagcaga tcggccagat caaggagctg
     7201 gtcttcaaat tcatggacga cgagactccg tcctgcggca tccggctcga taccccggcg
     7261 cccatccgca tgaatatgga acgccgcctc aacctggtgg aaaaacaggc cgccggcgtc
     7321 gtaaaacaac ccgccaacgc ccgcgaaaag gtggaggacc tggaacgctt cggccgcctc
     7381 ctcaatacaa cgcatgtcga caggaaaaag ctctggcaaa aggtggagac cgtcctcgaa
     7441 caaaagacta ccgccaccct cgcggagatc atcggcatca ccgggctgga acatggcgtc
     7501 gccgaggtcg tcggctattt cagcttcctg cgcgaaaaag cagcaaaggt gcagtccatg
     7561 caggagataa ccgaactgat cccgctggac gaggagaaga cccggttcat agaagttccc
     7621 tacctgctgt tcagcaggta accatcggcc cgcggcgccg taaagcaagc ccgcaacaaa
     7681 agaaacacag acatgaacac agataaacaa aatatcttac cgtatacccc ggtattcata
     7741 aagctcctga aatcaccggt ggagtatctc gaaaagaccg cctgggaaca actgctccag
     7801 tatcagggag agctgacccg gtttctccag cagctggggc tggtgctcgt actggaaaaa
     7861 gaggacgggt acgcctacct cgagcaggca aggctcgacg aggaagagaa tgtcatcggc
     7921 tgggtacggc ggatccagct gggctatgaa gagagcgttc tgctcgtgct gctccgcgat
     7981 atgatggccg agttcgaagt aggggaggcc ggcgcccgcg aactgatcag gaaacgccgg
     8041 gagataaaag aatatgccga gctcttcttc cgcgagaacg ccagccgcgt aaagttcatc
     8101 cgcgatctgg accggctgat cgatcgggtc gaggaactcg atttcctgga gaaagtagaa
     8161 cagcacgaac tacaggacga gcagaaattc cgggtgaaga agatcataaa agcacgcgta
     8221 gacaacgaga tattggaaaa cttcaaacaa caactatccg agcatgcagt tgggcgtatt
     8281 tagtaccgat gagaacaaat ccgggttccg tttgcaatac atggagctct tcaactgggg
     8341 aactttcgac gaacatgtct ggaagatcac acccggcgga gagacatccc tgcttaccgg
     8401 cgccaatggc agcgggaaga cgacctttgt cgatgcactg ctgaccctga tcgtccccga
     8461 aaagaaatac cggttttaca accaatcctc cggcagcgag aaaaaaggcg acagaacgga
     8521 agaatcctat gtgctgggcg gatacgggag cgtccatagc gacaacggcc tctccagcaa
     8581 gaccctttac ctccgggaca ataaggagca ggcctatagc atcctgcttg cccatttctc
     8641 caacgaggcg gaacagtcgg tcaccctttt ccaggtgcgc tggttcagcg gtcatgagat
     8701 gcgccgcgtc tttgccatcg cgcacaaacc cctgcacatc gaggacgact tcaaaccctt
     8761 cgatctcggt ggcgcctggc ggcggcggat cgaccagcaa tacaataaag gcagccgcaa
     8821 acaggtggaa tggttcgacg cggccagccg ctatgcccaa cggatggtgg aagtcctggg
     8881 aatgcaaagc atgcaggccc tgagtctctt taaccagacc atggggatca aggtcctggg
     8941 cgatctgaac gagttcatcc gcggcaatat gctggaaccc cgcaatatgg aacaggagtt
     9001 ccaggaactc aaaaaacatc tctccaccct gctggatgcc cagcgaaaca tcgagaagac
     9061 cggggagcag gtacggctgc tccacccgct gaagaaacat ttcctcgcat acaaagaaca
     9121 aaccgatctc ggcgcggccg cccggcagga actggatacc gcccgcatct ggaagaacta
     9181 taccaaatac cacctgctcg acgaatacat ccgcagccag gaaaaagatg ccggcaatct
     9241 gcggcgggat tcggaagagg caaaacaaaa gatcaccacc ctgctcgaag aagaaagaca
     9301 gacgctgaac cagctggagc aaaataaggc gggccagcgg ctgcaacagc tgcaaaaaga
     9361 aagggaaggc ctcgaacaac aggtaaaaga aaccgaagcc gccctgaaaa aattcgccga
     9421 ctggtgtgca ctgttgcacc tcgagacgag ccccgccgac gaggccgcgt accagcggat
     9481 caagaaagaa gccgagcgca ccgataaaaa attagccacg gagatccgca acaataacga
     9541 agacctgtac gaagcgcagg atcggcaaaa gaaagcaacg gaagaaaaga cgaagatcga
     9601 gggcgaactg aataccctgc tccagagccg gaataatatc cccgcccatc tcgtggccct
     9661 ccgccgcgat ctctgcgccg aactcaaaat agacgaccag gaactcccct tcgccggcga
     9721 actgatccag gtccgcaacg aggaaatgga ctggcagccg gcattggaaa aactactgaa
     9781 ttctcttgcc cttcgtttgc tggtcccgga gaaacattac cggaaggtca gccgctacgt
     9841 caacaataca cagctaaagt cccggctcgt ctatcacctg ataaaagaca ttgccctgca
     9901 acaacacgcc gatcccggca cggtctggca caagctggag tttcaacccg ggcatccgct
     9961 cagcagctgg gtaagccagc aggtcatcca gcaata
//
LOCUS       DATGFG010000624         6869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1606708, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000624 DATGFG010000000
VERSION     DATGFG010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606708"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VKQ52_11685"
     CDS             <1..672
                     /locus_tag="VKQ52_11685"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HLZ87900.1"
                     /translation="QPGDYRVELYFAEPWLGAGSPKLDCTGWRTFDVAVNGQPVIKNL
                     DIWKEAGRATALKKIVDVHVTGNKLDIGFPHIESGQALIAAIAIATKDRKQVPAPPSV
                     DTATYRLEPAYDTRPVKTYKNAEAGITGDTTEWNIEVGVGDKYSLTIRYRWQNPDATA
                     RLEVRMADGTLINTEPVTLKTTLPSKWNYISSTTGTMINAGHYIIRLIVPAKENLRID
                     ELQVQ"
     gene            757..4863
                     /locus_tag="VKQ52_11690"
     CDS             757..4863
                     /locus_tag="VKQ52_11690"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HLZ87901.1"
                     /translation="MKRLLTITFLLLAAAAAATAQEIKLTNLRTEYRTNPLGIGTLTP
                     RLSWELASSHHGIKQSAWRILLYLDTVTNLFWDSQKQPGAASIGTIYSGPPLTAGHTY
                     YWKAMVWDEKRNDSSKFSAINSFQTGLISPADWKGAQWIAYSQLPDTSLRLPALTTKK
                     PAPGRDTLPIFQKQLTITKPLLRATAFISGLGHFELSVNGEKTGDHFLDPGWVTYDKK
                     ALYVALDLTAQLQQGGNTLRVLLGNGFYYIPRERYHKLLVAYGYPKLIARIVLEYKDG
                     STEDILTNSSWQTALGPITFSSIYGGEDYDARREQPGSQEGPDGIAQTWRPAIVVQGP
                     PRLEAQSAEPLKVFEHFEPRSVTRRTPTSYIYDLGQNASGIAAITLTGQKGATVRITP
                     AELLAGDSANQKATGKPYYWEYTLKGGQPESWQPRFTYYGFRYLQLDGAVPAGQPNPG
                     NLPVVTQLTGLHTRNAMERTGKFTCSNELFRRIDTLIDWAVQSNLASVFTDCPHREKL
                     GWLEEAHLMGNSLRYGYDIASLYRKVIRDMMDAQTDSGLIPEIAPEFTVFGAPFRDSP
                     EWGSSAILLPWYCYQWYGDKGALEEAWPMMQRYIAYLARQAQDHLLYEGLGDWYDIGP
                     NHPGVSQLTTKGITASAIYYYDLTIMAQTATLLNQPDQGREYTAQAEAVKQAFNDKFL
                     GTKTGNIGSGSQAANAMALYTGIVPRQYRDRVFANIVKDLHQRDNSLTAGDIGFRYLL
                     KMLDEAHRPDLIYDMNNRTDVPGYGYQLAKGATALTESWQAYPSVSNDHLMLGHLLEW
                     FYEGLAGIHALDSPAHSTIHPEPVGNIQWAGADYHSPYGPISAHWKKGANTFDLEVTI
                     PGNTTATVWLPAPTGAVITEGWKRLSLRPDVHLIRRDSGWAVFELGSGSYTFRAVTGN
                     TVSEKAMAAIYEKIKTPYKYGLVLVPPDKNKKLDCPSVFRKGDSWYMSYIIYDGRGYE
                     TWLAKSADLLHWTTEGRIMSFTDTTTSSTPNSPSTPATPNFPAAATTSAGTPNSTTAP
                     TTPAAISDSAGVGMPRKAPRMVDSNHPKSQSKGMRSAGGDVPTRPSGEAPNSPTAKSS
                     GEAPSSPTANPGGEARNNWDANQKAGYISLQDITWGGTYQLQQYNGKYWMPYFGGTTT
                     GYEAGTLSEGMAFTDKDPATPHEWQRLDHPILSPRDTNRSWWDNHTMYKSWVVWDKAR
                     HTGHPFLLYYNANGDSLDRKRGAERIGMAVSDDMIHWSRFGRDPILDHLTGITGDPYI
                     QKIGDIYALFYFGAFWKGTHAAFNRFACSYDLVHWTDWTGPDLIEPSETYDEVFAHKS
                     FVINYKGIIYHYYCAVDKKGNRGIAVATSTDKGKSELTFDRTKSGPQN"
     gene            4888..>6869
                     /locus_tag="VKQ52_11695"
     CDS             4888..>6869
                     /locus_tag="VKQ52_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HLZ87902.1"
                     /translation="MRIFLAVGVCLLLSSAATPQTPETNQIPHQRLNFDAGWKFHPGD
                     DPSASAPAFNDKPWRSLDLPHDWSIEGEFSQNNSTGQQEGGLPAGIGWYRKTFKLPSG
                     ITPAVTEGQAGAGMPGMAHRMVDSNNPKSQAKGMRATGPDVPVRPQLSPNVPVRPGIS
                     GDKIYISFDGIYRNSEVWINGHLLGKRPNGYISFRYDLTPWLKKDADNVLAVRVDNSA
                     QPNSRWYTGSGIYRHVWLETLPAAPIAIDHWGIQVSTGDITDQQARLRISTTLRSAQT
                     GQSCTLQQVLYDANGNKVTTLTLPYTAKDTLNTVQQDLWFPAAQRWSPEHPYLYTLET
                     SLFIGNRLIDRQMTRTGIRQFQFDFRQGFLVNGHHLKIKGVCLHHDLGALGAAVNTSA
                     MRRQLLILKEMGCNAIRTAHNPPAPEFLDLCDEMGFLVMDEAFDMWQKKKNKYDYSLD
                     FAQWHRRDLEDQVRRDRNHPSIFIWSIGNEIREQFDASGITLTKELTGIVRSIDPTRP
                     ITSAMTETDTLENNIYQAGVLDVMGLNYNQQVYDSAQRKYPGQPFLATETTSAEATRG
                     HYDMPSDSIRRWPTSAKVPAVGVNRDYTVSAYDDVSAYWGSTHEETWRIVKRDPFVAG
                     LFVWSGFDYLGEPTPYRWPARSSYFGIVDLAGFPKD"
BASE COUNT         1656 a         2480 c         1599 g         1134 t
ORIGIN      
        1 caacccggcg actaccgcgt cgaactctat tttgcagaac cctggctggg cgccggcagc
       61 ccaaaactcg actgcaccgg ctggcgcaca ttcgatgtcg ctgtcaacgg acagccggtg
      121 ataaaaaacc tcgacatctg gaaagaagcc ggccgcgcca ccgcgttaaa aaagatcgtc
      181 gacgtacacg tcaccggaaa taaactcgac atcggcttcc cgcacatcga gtccggacag
      241 gccctcatcg ccgccatcgc catcgcgaca aaagatcgaa aacaggtccc ggctccccct
      301 tccgtcgaca cggccaccta ccgcctcgaa cccgcctatg acaccaggcc cgtgaaaaca
      361 tacaaaaatg ctgaggccgg aatcacaggc gatacaacgg aatggaacat cgaggtcggg
      421 gtaggggata agtattccct cacgatcagg tatcgctggc agaaccccga cgccacagcc
      481 agactggagg tccgcatggc cgacggcacc ctcatcaata ccgaacccgt gaccctgaaa
      541 acaaccctgc cgtcaaaatg gaactatatc agcagcacca ccggcaccat gatcaacgcc
      601 ggtcattata tcatcaggct catcgtcccg gcaaaagaaa acctccggat cgacgaactg
      661 caggtccaat aaaacaagac gaaccactga tctcaaaaaa caagatgaac cactgatcca
      721 ataaacaaga tgaaccactg atccaataaa gcaaatatga agcgactcct caccataaca
      781 ttcctcctcc ttgcagcagc agccgcggca acagcacagg agatcaaact caccaacctc
      841 cgtaccgaat accggaccaa ccccctgggt atcggcaccc tgaccccgcg tctcagctgg
      901 gaactggcat cctcccatca cggcatcaaa caatccgcct ggcgcatcct gctctatctg
      961 gacacggtca ccaacctctt ctgggactct caaaaacaac caggcgccgc ctccatcgga
     1021 acaatatact ccggtccacc actcaccgcc ggccacacct actattggaa agcgatggtc
     1081 tgggacgaaa aacggaacga cagctccaaa ttcagcgcaa taaactcctt ccagaccggc
     1141 ctcatcagcc ctgccgactg gaaaggtgcg caatggatcg cctacagcca actccccgac
     1201 accagtctcc gccttccggc actcactaca aaaaagcccg ctcccggcag ggacaccctc
     1261 cccatctttc aaaaacagct gacgatcacc aaacccctcc tccgcgccac cgccttcatc
     1321 agcggtctcg gtcacttcga gctcagcgtc aatggcgaaa agacaggcga ccacttcctc
     1381 gatcccggct gggtcaccta tgataaaaag gcactctatg tagccctcga cctcacggca
     1441 cagctgcaac agggcggcaa caccctccgc gtcctccttg gtaacgggtt ctattacatc
     1501 ccgcgcgaac gctatcacaa actcctcgtc gcctacggct atccaaagct catcgcccgc
     1561 atcgtcctcg aatacaagga cggcagcaca gaggacattc tcacaaattc cagctggcaa
     1621 acagccctgg gacccatcac cttttccagc atctacggcg gcgaagacta cgacgcccgc
     1681 cgcgaacaac ccgggagcca ggaaggcccg gacggcatcg cccaaacctg gcgcccagcc
     1741 atcgtcgttc aggggccgcc cagactagag gcccagtccg ccgaaccgct caaggtcttc
     1801 gaacacttcg agccaagaag cgtcaccagg cgaaccccca cctcctacat ctacgatctc
     1861 ggccagaacg cgtccggtat cgccgccatc accctcaccg gacaaaaagg cgccaccgtc
     1921 cgcatcaccc ccgccgaact cctcgccggc gacagcgcca accagaaagc caccggcaaa
     1981 ccatattatt gggaatacac gctaaaaggc ggacaaccgg aatcctggca accccgcttc
     2041 acttattatg gcttccgcta tctccaactc gacggcgcgg tacccgccgg ccagcccaac
     2101 cccggcaacc ttccggtagt cacccaacta accggcctcc acacccgcaa cgccatggag
     2161 cgtacgggca aattcacctg tagcaacgaa ctcttccgcc gcatcgacac cctcatcgac
     2221 tgggccgtcc aaagcaatct cgccagcgtc ttcaccgact gcccccaccg cgaaaagctg
     2281 ggctggctcg aagaggccca tctgatgggc aactccctgc gttatggcta tgatatcgcc
     2341 tccctctatc gcaaggtcat ccgcgatatg atggacgccc agaccgacag cggcctcatc
     2401 cccgagatcg cccccgaatt caccgtcttt ggcgcaccct tccgcgactc tcccgaatgg
     2461 ggcagcagcg ccatcctgct tccctggtat tgctaccagt ggtacggcga caagggcgcc
     2521 ctggaggagg cctggcccat gatgcagcgc tatatcgcct atctcgcccg ccaggcgcag
     2581 gatcatctgc tctatgaagg gctcggcgac tggtacgata tcggccccaa ccaccccggc
     2641 gtttcccaac tgacgacaaa aggcatcacg gcaagcgcca tctactacta cgatctgaca
     2701 atcatggccc aaaccgccac cctgctgaac cagccggacc aaggccgcga atacacggcg
     2761 caggccgaag ccgtaaagca ggccttcaac gacaaattcc tcggcactaa aaccgggaat
     2821 atcggcagcg gcagccaggc cgccaatgcc atggccctct acacgggcat cgttccgcgg
     2881 caataccggg acagggtttt cgccaacatc gtaaaagacc tccatcagcg cgacaacagc
     2941 ctcaccgccg gcgacatcgg cttccgctac ctgctaaaaa tgctcgacga agcccatcgc
     3001 cccgacctca tctacgacat gaacaaccgt accgatgtcc cgggctacgg ctaccaattg
     3061 gcaaaaggcg ccacggcgct caccgagtcc tggcaggcct atccctccgt ttccaatgat
     3121 cacctgatgc tcggccacct cctcgaatgg ttctacgaag ggctcgccgg tatccacgcc
     3181 ctcgacagtc cagcccacag tactattcac cccgaacccg tcggcaatat ccaatgggcc
     3241 ggcgccgact accacagccc ctatggcccc atctccgccc actggaaaaa aggggccaac
     3301 accttcgatc tcgaggtcac gatccccggt aatacgacgg ccaccgtctg gctccccgcc
     3361 ccgacgggtg cggtcatcac cgaaggctgg aaacgcctct cgctccggcc cgatgtccat
     3421 ctcatccgcc gggacagcgg ctgggccgtc ttcgaactgg gctcgggcag ctataccttc
     3481 cgcgccgtca ccggcaacac cgtcagcgaa aaagcgatgg ccgccatcta cgagaagata
     3541 aagaccccct acaaatacgg cctggtcctg gttcccccgg acaagaacaa aaaattggac
     3601 tgccccagcg tcttccgcaa gggcgactcc tggtatatgt cctatatcat ctatgacggt
     3661 cgcggctacg agacctggct ggccaaaagc gccgacctcc tccactggac gaccgagggc
     3721 cggatcatgt ccttcacgga tacaacaact tcctccacgc ccaactcccc atccaccccc
     3781 gccacgccca acttcccggc cgccgcaacc acctctgccg gaactcccaa ctccacgacc
     3841 gccccaacca cccccgccgc catcagcgac tcagccggtg tcgggatgcc ccgtaaggcg
     3901 cctcgcatgg ttgactccaa ccatccaaaa agtcaaagca agggaatgcg cagcgccggc
     3961 ggggacgtcc cgacccggcc cagcggtgaa gccccaaact cccccaccgc gaagtccagc
     4021 ggagaagccc caagctcccc caccgcgaat cctggcggag aagccagaaa caactgggac
     4081 gccaaccaaa aagccggcta catctccctc caggacatca cctggggcgg cacctaccaa
     4141 ctacagcaat acaacggcaa atactggatg ccctatttcg gcggcaccac cacgggctat
     4201 gaagccggca ccctctccga agggatggcc tttacggaca aagatccggc caccccccac
     4261 gaatggcaac ggctcgacca ccccatcctg agcccaagag ataccaacag gtcctggtgg
     4321 gacaaccata ccatgtataa aagctgggtc gtctgggaca aggcccgcca caccggccac
     4381 cccttcctgc tctactataa tgccaacggg gacagcctgg acaggaaaag aggcgccgaa
     4441 cgcatcggta tggccgtctc ggatgacatg atccactggt cccgtttcgg gcgggacccc
     4501 atcctcgatc acctcacggg catcaccggc gacccgtata tccaaaaaat aggcgatatt
     4561 tacgccctgt tctatttcgg ggccttctgg aaaggcaccc acgccgcctt taaccgtttc
     4621 gcatgttcgt acgatctcgt gcactggacg gactggacgg gccccgatct gatagaaccc
     4681 tccgaaacat acgatgaagt ttttgcccac aagtcttttg tcattaatta taaaggcatc
     4741 atatatcatt actattgtgc cgttgacaaa aaaggcaatc gcggcattgc agtggctacc
     4801 tccaccgaca aagggaaaag cgaattgaca tttgaccgga cgaagtccgg tccccaaaat
     4861 tgattttagt agatagtaga aaagagtatg cgtatatttc ttgccgttgg cgtttgttta
     4921 ttgctttcaa gtgccgcgac accccaaacc cccgagacaa accagatacc ccatcagcgc
     4981 ctgaacttcg acgccggctg gaaattccac cccggcgacg acccgtccgc cagtgccccc
     5041 gcatttaacg acaaaccctg gcgcagcctc gacctccccc atgactggag catcgaaggc
     5101 gaattcagcc agaacaattc cacgggtcaa caggaaggcg gcctccccgc cggcatcggc
     5161 tggtatcgca aaaccttcaa attaccctcc ggcatcacac ccgccgtcac cgagggccag
     5221 gccggtgccg ggatgccggg catggcgcat cgcatggttg actccaacaa tccaaaaagc
     5281 caagccaagg gaatgcgcgc gacaggaccg gacgtcccgg tccggccgca actctccccc
     5341 aacgtcccgg tccggccagg aatctccggc gacaaaatct atatctcctt cgacggcatc
     5401 tatcgcaaca gcgaggtctg gatcaatggc cacctcctcg gcaagcgccc caacggctat
     5461 atctcctttc gctatgacct caccccctgg ctgaaaaaag acgccgacaa cgtcctcgcc
     5521 gtccgcgtcg acaactccgc acaacccaac tcccgctggt ataccggctc ggggatctac
     5581 cgccacgtct ggctggaaac cctccccgca gcacccatcg ccatcgacca ctgggggatc
     5641 caggtctcca ccggcgacat aaccgatcag caggcccgcc tccgcatctc caccaccctc
     5701 cggtccgccc agaccgggca gtcctgcacc ctgcaacagg tgctctatga tgcgaacggc
     5761 aataaggtga cgaccctcac ccttccgtac accgcaaaag ataccctcaa caccgttcag
     5821 caggatctct ggtttccggc agcccagcgc tggtcccccg aacatcccta tctctatacc
     5881 ctcgagacca gcctctttat cggcaacaga cttatcgatc gccaaatgac ccgtaccggc
     5941 atccgccagt ttcaattcga tttccgccag ggcttcctcg tgaacgggca ccacctcaag
     6001 ataaaaggcg tctgtctcca ccatgacctc ggcgcccttg gggccgccgt caacaccagc
     6061 gccatgcgcc gccaactcct catcctgaaa gagatgggtt gcaacgccat ccgcactgca
     6121 cacaatcccc ccgcacccga attcctcgat ctctgcgacg aaatgggttt cctcgtcatg
     6181 gacgaggcct tcgatatgtg gcagaagaaa aagaacaaat acgactattc cctcgacttc
     6241 gcccagtggc atcgccgcga cctcgaagac caggtccgcc gcgaccgtaa ccacccctcc
     6301 atcttcatct ggagcatcgg taacgaaata cgggaacagt tcgacgccag cggcatcacc
     6361 ctgacaaagg aacttaccgg tatcgtaaga tcgatcgacc ctacccgccc catcacctcc
     6421 gccatgaccg agaccgacac cctggagaac aatatctacc aggccggcgt cctcgatgtc
     6481 atgggactca actataatca gcaggtgtat gattcggcgc aaaggaaata tcccggccaa
     6541 cccttcctcg ctaccgagac cacctcggcc gaggccaccc gggggcacta cgatatgccc
     6601 tccgacagca tccgccgctg gcccacctcc gcaaaggtcc ccgccgtcgg cgtcaaccgc
     6661 gactataccg tttccgccta tgacgacgtc tcggcctact ggggctcgac ccacgaagag
     6721 acctggcgca tcgtaaaacg ggaccccttc gtggcggggt tgtttgtgtg gagcggcttc
     6781 gattatctcg gtgagcccac tccctatcgc tggcccgccc gcagctccta ttttggcatc
     6841 gtcgacctcg ccggcttccc caaggacag
//
LOCUS       DATGFG010000625         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1097462, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000625 DATGFG010000000
VERSION     DATGFG010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097462"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VKQ52_11700"
     CDS             <1..357
                     /locus_tag="VKQ52_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87903.1"
                     /translation="QQTRFEDALQLDYNVTGRPDGKTIAPLLLITFVENAFKHGVNPE
                     QPSAIRIRIDIGEKELRLEVANKKVVAKLLPSEPIGLGIKNTRQRLGILYPGQYTLSI
                     EEGEKDFHVLLTLHLT"
     gene            354..1052
                     /locus_tag="VKQ52_11705"
     CDS             354..1052
                     /locus_tag="VKQ52_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ87904.1"
                     /translation="MIKAIAIDDEAPALKVIANFCDRTDAISLECTFRRPAEALNYLH
                     QSPVDLIFLDIQMPSQSGIEFYKTVGPGTLAIFTTAHSQYAVEGFNLNAIDYLLKPFT
                     LERFQQAVNKAVQFLAFRDTQQGQGQFSEALLIRADYSLIRINTAEILFVEAADDYLK
                     IFQETKPPITVRMTMKSLVTMLSPADFMRIHRSYIVSLRRIDNVRNKMISLGGRDIPI
                     GNNYEAAFFRQFGR"
     gene            1155..1922
                     /locus_tag="VKQ52_11710"
     CDS             1155..1922
                     /locus_tag="VKQ52_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HLZ87905.1"
                     /translation="MEQNTRARFLKSLLIGATAVPVLVEACSKSGNSSSSSSSSSSSS
                     STSGSTDSTYTGTSSSSCSATPSETAGPYPYDLSSNSAIFRTNITEGKTGIPLSLTLT
                     IVNSNSSCAVIPGARVDIWHCDKDGYYSEYSEPGYLGTQDNTGKTFLRGIQLADTNGQ
                     VKFTTIYPGWYTGRVTHIHVEVFVNSVLKLTTQLAFPDSLNATIYGTSLYSAHGQNTI
                     TNSSDQVFSDSYSSELVTISGNTTDGYTATFKVGVPL"
     gene            2014..2547
                     /locus_tag="VKQ52_11715"
     CDS             2014..2547
                     /locus_tag="VKQ52_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87906.1"
                     /translation="MSNAIIRLAYRQIIDMHSITPFEQKIFSVTFAEFGIQQQSFSKG
                     MELYTWESIRNHFPKSQQALPFKVSFSIAGLISSLQNKIPGLEDALGNENIPFTQHRF
                     SLIASDVRDPSVHAVSLTWITDDLILHGIIGDQLLLSQQPPQTFQVKMQPGLSVISYT
                     PCEERSTRQPFATMASL"
BASE COUNT          696 a         1035 c          576 g          536 t
ORIGIN      
        1 caacaaaccc gcttcgaaga cgccctccag ctggactaca atgtcaccgg ccggccggat
       61 ggcaagacca tcgcccccct cctgctcatc acctttgtgg aaaacgcctt caaacatggc
      121 gtcaacccgg aacaaccctc cgccatccgc atccgtattg acatcgggga aaaggaactc
      181 cggctcgaag ttgctaataa aaaggtagtc gccaaactat tgccctccga acccatcggc
      241 cttggcatca aaaacacccg ccaacgcctc ggtatcctct accccggaca atacaccctc
      301 tccatcgaag agggagagaa agatttccat gttttgctaa ctttacacct gacatgatca
      361 aagccatcgc tatagacgac gaagcgccgg cactaaaagt gatcgcgaat ttttgcgacc
      421 ggacagacgc catcagcctc gaatgcacct tccgcaggcc cgccgaagcg ctgaactacc
      481 tgcaccaatc tcccgtcgac ctcatcttcc tcgacatcca gatgccctcc cagtcaggga
      541 tagaattcta caagaccgtc ggccccggca ccctcgccat cttcactacg gcccacagcc
      601 aatacgcggt agaaggtttc aacctgaatg ctatcgacta tctgctaaag cccttcaccc
      661 tcgaacgctt tcagcaggca gtgaacaaag ccgtccagtt cctcgccttc cgcgacaccc
      721 agcaaggcca gggacaattc tccgaagccc tcctcatccg cgctgactat agcctcatcc
      781 gcatcaacac cgccgagatc ctcttcgtcg aagccgccga cgactacctg aagatcttcc
      841 aggagacaaa acccccgatc accgttcgca tgacgatgaa aagcctcgtc accatgctct
      901 cccccgccga cttcatgcgc atccatcgct cctatatcgt atccctccgc cgcatcgaca
      961 acgtccgcaa taaaatgatc tccctcggcg gccgcgatat ccccatcggg aacaactacg
     1021 aagccgcctt cttccgccaa ttcggccgtt aaccgcagga ttccccgcct ttacctcatc
     1081 taagctgtta aagcgatttt ccccggcaac ttgactttcc acgcccgcgt tactccaaca
     1141 aatactccac taccatggaa caaaacacaa gggcaagatt cttaaaaagc ctgctcatcg
     1201 gggctaccgc cgtgcctgtc ctcgtagagg catgcagcaa aagcggcaac tcatccagca
     1261 gcagcagtag tagcagcagt agcagcagca cctcgggttc aacggacagc acctacacgg
     1321 gcacatccag ctcgtcctgc tccgccaccc cctcggagac agccggcccc tatccgtatg
     1381 acctcagcag caactccgct atcttccgca ccaacatcac ggaaggcaag acaggcatcc
     1441 ccctctcgct gaccctgacc atcgtcaatt ccaacagcag ttgcgcggtc atcccgggcg
     1501 cccgcgtcga tatctggcat tgcgataagg acggctatta ctccgaatac agcgaacccg
     1561 gctacctggg gacacaggat aacaccggca aaactttcct gcgcgggatc caactcgccg
     1621 ataccaatgg ccaggtaaag ttcacgacca tctatcccgg ctggtacacc ggccgcgtga
     1681 cccatatcca cgtcgaggtc ttcgtcaact ccgtgctgaa actcaccacg cagctggcct
     1741 tccccgacag cctgaatgcg accatctatg gcacctcgct ctattcggca catggacaaa
     1801 acacgatcac caactcttcc gaccaggtgt tctctgactc ttacagcagc gaactggtca
     1861 ccatcagcgg aaataccacg gacggttata cagccacatt caaagtggga gtgcccctct
     1921 gaaacactag tacttcaaac agggatgcgg agcaacggcc tggtcccgca aggggccagg
     1981 ccaattttta aaaaagaaaa aaaacaatca actatgtcaa acgcaatcat tcgcctcgcc
     2041 tatcgccaga tcatcgatat gcactcgatc acccccttcg aacaaaagat cttctccgtc
     2101 accttcgccg aattcggcat ccagcagcaa tccttctcaa aaggcatgga actgtacacc
     2161 tgggagtcca tccggaatca tttccccaaa tcccagcagg ccctgccgtt caaggtcagc
     2221 ttctccatcg ccgggctcat cagctccctc caaaacaaga tccccggcct ggaagacgcc
     2281 ctcggcaacg agaatatccc tttcacacaa caccggttca gcctcatcgc ctccgacgtc
     2341 cgcgacccat ccgtacacgc ggtcagcctg acctggatca ccgacgacct catcctccac
     2401 gggatcatcg gcgaccaact cctgctctcg caacaaccac cccagacttt tcaagtgaaa
     2461 atgcaaccgg gcctgtccgt gatcagctat accccctgtg aggaacgcag cacccgccag
     2521 cccttcgcaa cgatggcctc gctatagccc cctgcccgcc cccggccgct ctgccttccg
     2581 ccccccacac acgtcaacgc aaacctcaca cgcgccaacc ccaccctgat cggcgcgcac
     2641 gttcccttcc gcccttacac acgtcacctc aaacctcact ggcgcgtcaa cgccatccct
     2701 cacacactcc aactccaccc tgatcggcgg gtactctccc gccgtcccgg cgccggtcat
     2761 aacttttgtt atattgaccg gaagggccgt ttacactact ttgccgccct taacatacac
     2821 gcatgaacgt tacactttct tct
//
LOCUS       DATGFG010000626         9082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_389823, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000626 DATGFG010000000
VERSION     DATGFG010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389823"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VKQ52_11720"
     CDS             complement(<1..1466)
                     /locus_tag="VKQ52_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87907.1"
                     /translation="MTPSYKPAAYIQWIVLALFLFFFWPAYFYQAPSTGIDPSWSIAL
                     HLARQSHLVFGREFIFTYGPLGILYCRLPIGVNLLFYLLSDLFYLGSFLWIVKRLLKE
                     NAGAGTLIYLFLCVLAGAYISINLWYFIFFLYFLFSFLREKENTALLIQAALLSVISF
                     YIKVSSGMTAIGIFLACLVFCVVFARMTIRYFLVVLLAYLLFIFLSALVLHVALVDYI
                     RVNLQLIGFYNDSMMRPIGADYRAFLYPAVFTVLIAVVWFAVRLGRAFLKKKIREDAG
                     LLFIYGTVALSAFVFFKGAFVRSDTHIYHFYEYIGPLAGLLFLFTPRGERRLAAIACW
                     VILLPALWAINALPGSRRPYTRLVTLGIFTDKAGEIGTYFKGIGRYNSELRHSDSLRY
                     SDNPYRKIIGDSTVDIMPHEVSTLYFNGLRYDPRPVIQSYSAYSEYLDKLNYAKYTSP
                     DAPQYVLFNIGSVDDRVPFFDETRTKLALLDRYEVAGR"
     gene            1538..2491
                     /locus_tag="VKQ52_11725"
     CDS             1538..2491
                     /locus_tag="VKQ52_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ87908.1"
                     /translation="MKITAVVVTFNRLGLLKECIAGLKRQTAPLHEIVVVNNGSSDGT
                     DVWLAGEQGLTVVTQGNLGGSGGQYTGISKAMERDPDWVWLMDDDAEPYPDALEKLLH
                     FLDADTVAAAACSVIDNANRISLVHRGEYDYRRLTSEYGCRPTPETEYARDSVDVGYA
                     TFVGIIVNSRAVKTIGLPKKELFLHFDDIEYSLRLRKFGRIVLVPASRMLHKENASTH
                     FYTKKVLGKPRVRVKYEKLWLRYFVIRNVIWGVKEYHGKKWDTPFVLANYYIRSVVGI
                     LLFDDHKWKRIVFLTGAFRDGLSAHFENSYDYINGRTGLYP"
     gene            complement(2527..3468)
                     /locus_tag="VKQ52_11730"
     CDS             complement(2527..3468)
                     /locus_tag="VKQ52_11730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ87909.1"
                     /translation="MPECRICKNDKGIKDVYRVKEMMFGLDEYFEYFLCPVCNCLQIV
                     AFPEDMGKYYGAAYYSHSPDKSLLKRLSTRMAEWRDKADLFNEHFLGRIIALFVPGRE
                     EIRFISYLGGIHKHSRILDVGSGMGFYLHRLEALGFTDLRGIDPFLKEEIVHSGKVTI
                     QRKYLADYEEKDFDLITLQHTFEHLPNPQETLKMVYDRLVPGGYCVIRVPVIPNMAWE
                     EYRENWFQIDAPRHIFIPSVDTMERLCGPVGFRMDGIVYDSTPKQFYISEQYRQGIPL
                     IKQKNAISPEKMAEYRRKTFVANKTGKGDQAIFILRK"
     gene            complement(3461..4684)
                     /locus_tag="VKQ52_11735"
     CDS             complement(3461..4684)
                     /locus_tag="VKQ52_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87910.1"
                     /translation="MKINRYFPFVFVYFFINSVGLPWGLLYTTLLSPLLYIWVVRTRR
                     KEVLFPFLAVSALFFAGWAFTGMDMGKFLVSWANMMGVYIFAQAVYTFLKTCQDKERI
                     FWRILVVNFAFCLIAIPFYFTPWADIFWIEQVYTEGVNSLKRFKLFTYEASYYATMFT
                     PVFLYFFYQVMLKQNKRGLLGMALLLTVPYLLSLSMGVIICLCAACGLTYLLYFGPLT
                     RKKRLFHLVIAGVVLMIAGVLVLVVFFPENALVVRTENVFSGRDISTQGRTTDAFYLA
                     QRILERRNAWCGIGPGQLKLIGADIIRDYYSYDQDFDIITIPNAMAETLVVFGWIGLF
                     ARLGIEIGLFVYTKVWKNYYRLALFLFMFLYQFSGSFITSTAEYVIWILAFTNVFPQF
                     DIKLPSISKNKLTNA"
     gene            complement(4671..5600)
                     /locus_tag="VKQ52_11740"
     CDS             complement(4671..5600)
                     /locus_tag="VKQ52_11740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ87911.1"
                     /translation="MANNVYIIIVNYKKWTDTRDCLESLFRSTHQPFTVIVTDNASGN
                     GSLESLAAWAEGRTDYRLFQRAEWEQEGIVPAELPRLVLLQNDRNKGFAEGNNVAIRA
                     LLTEKAYVWLLNPDMTVEENTLSTLVGFAGSRPFRAITGSVIKYFDPPHAIHLYGGGS
                     INWRSGTVELLQEEADLPRLDYISGGALFTHTAHFNEIGLLPEEYFLYWEETDWCSRA
                     KERGYLLQVCPDAICYDKISTTIGRGFMADYFYTRNGLLFLSKFRKDPSGLAMSLTFL
                     RFLKRVSTGKWKRAWGVYKGYRSFLKMRSNENK"
     gene            complement(5593..6714)
                     /gene="glf"
                     /locus_tag="VKQ52_11745"
     CDS             complement(5593..6714)
                     /gene="glf"
                     /locus_tag="VKQ52_11745"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HLZ87912.1"
                     /translation="MDKYDFLIVGAGLFGAVVAHEARKRGKTCLVVDRRPHIGGNTYC
                     EDEEGINVHKYGAHIFHTNDERVWKYVNSFVEFNRYTNSPLACYKGELYNLPFNMNTF
                     YQLWKVRTPEEARAKIEEQKGQWQTKEPANLEEQALSLVGHDIYEKLIKGYTEKQWGR
                     KCTELPAFIIRRLPVRFTFDNNYFSDKYQGIPIGGYNRLTGALLEGCEVRLGVDFCRE
                     RNALAPLAHTIVFTGQIDEYFDYRFGQLEYRSLRFEHERLNLPNYQGNAVVNYTERDI
                     PYTRIIEHKHFEFGTQEHTVISREYPLEWRPGLEPYYPVNDDKNTRIFKQYRLLADAE
                     ENVLFGGRLAEYRYYDMHQIVAAALTSVRSLFPEAAING"
     gene            complement(6707..8143)
                     /locus_tag="VKQ52_11750"
     CDS             complement(6707..8143)
                     /locus_tag="VKQ52_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HLZ87913.1"
                     /translation="MIKRNLIYNSLLSVSQILFPLIIFPYTFKILEPPGIGLVSFADS
                     LTQYLISFAAIGIPVYGVIETAKVRHDPARLARLFSELVLIQLVISLFITVVFIGLIF
                     TVEKLAEHKLLYFLSCGTLLMNAFTVEWFFQGIERFKYITMRTISVRFLFLIAVFVLV
                     KKKEDLLLYYGLTFISAFLNGVINFSYARREVRLEFTGLSCMRHMRPLLHIFFSNVAI
                     SVYILLDTVILGFLADDTAVGYYATAIKICKIPLAFMTALSTALIPRISASLSSGNRE
                     YVQNLIQKSFAYIILISLPIGAGLLLLAPELITVLANRNYLPAIPVVRTMAPLTLIIG
                     LSNLFGIQILTSMGKEKLTLYAVTLGMILSVVCNLILIPRFSYMGTAYTTLLTEGLVT
                     IVTGWYALRHFRVALPYNILWQSGLACLLFYPCYYLAGLLVAEGLLRMLLVISMAATV
                     YFCTQYFVFRNIFVKQTIADLLTKVRYG"
     gene            8284..>9082
                     /locus_tag="VKQ52_11755"
     CDS             8284..>9082
                     /locus_tag="VKQ52_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87914.1"
                     /translation="MSDSPSFQKDTTDDLDLAPMLVKAITFIRQHGWKIMLSSLVGLV
                     MGGFLYYLLPHTYKARMVVQSSVLSNAQDLRVINNWDDLLNKNGYAILGAIWHQPYNV
                     VSELNGIKAEIIPGTDENSGIVIEAAVSDSSMLPGVQQGIIYGLENSEYIKQRVATRR
                     QNLEYQIAQTKQELAKLDSARSYLRSFSGGSEPANSRLVLDVSNVTSQRLSCYDRIAG
                     FEEKLKFTRGVYLLQEFVPAKRGKIIPAPVLPLLGFALGLLLSYCSVV"
BASE COUNT         2321 a         2363 c         2460 g         1938 t
ORIGIN      
        1 atccttcccg ctacctcata gcgatcgaga agggctaact tggttcttgt ttcgtcgaag
       61 aaagggaccc ggtcgtcgac gctgccgatg ttgaacagga catattgcgg cgcatccggg
      121 gaggtgtatt tcgcgtaatt gagcttgtcc aggtattcgc tgtaggcgga ataggactgg
      181 atcacaggcc gcggatcgta ccgcagcccg ttgaaataaa gggtggagac ctcatgaggc
      241 atgatgtcca ccgtgctgtc gccgatgatc ttccggtagg gattgtcgct gtatcgcagg
      301 ctatcggaat ggcgcagttc cgaattgtac cggccgatcc ctttgaaata ggtaccgatc
      361 tccccggcct tgtccgtaaa gatcccgagc gttacgagcc gcgtgtacgg ccgccggctg
      421 cccggcagcg cattgatcgc ccaaagtgcg ggcagtaaaa tgacccagca ggcaatcgcg
      481 gcaagccttc tttccccgcg tggggtgaag aggaagagga gacctgcaag cgggccgatg
      541 tattcataaa aatggtagat atgggtatcc gaccggacga atgctccctt gaaaaagaca
      601 aatgcactca gggctaccgt cccgtaaata aaaaggagcc cggcatcttc ccggatcttc
      661 tttttaagga atgcacggcc aagcctgacc gcgaaccaca cgacggcaat aaggaccgtg
      721 aatacggccg ggtagagaaa ggcccggtag tcggcgccaa tgggccgcat catcgaatcg
      781 ttgtaaaagc cgatcagctg gaggttaacc ctgatataat ccaccagcgc aacatgcagg
      841 accagggcgc tgaggaagat gaaaaggaga tacgccagga gcacgacgag gaaataccgg
      901 atcgtcatcc gcgcaaaaac aacacaaaaa acgagacagg ccaggaagat gccgatcgcg
      961 gtcatacccg agctcacctt gatataaaag gaaatgacag acagcagggc agcctggatc
     1021 agcagggctg tattctcctt ttcccggagg aaggaaaaaa ggaaataaag gaaaaagata
     1081 aaataccaca gatttatcga aatataggcc cccgccagga cacaaaggaa caggtagatc
     1141 agggtccccg ccccggcatt ctcctttagc agccgtttga cgatccagag gaaactaccc
     1201 aggtaaaaca gatcggaaag aagatagaag aggaggttga cgccaatcgg cagacgacaa
     1261 tacaggatgc cgagcgggcc ataggtgaag atgaactccc tgccgaacac aagatgggat
     1321 tgacgggcaa gatgaagcgc gatgctccag gacggatcga tgcccgtcga gggcgcctgg
     1381 taaaaatacg caggccagaa gaaaaaaagg aaaagggcaa ggacgatcca ttggatatag
     1441 gcggcaggct tgtaggaagg ggtcatcagg atatcagttt tggtgagcta tttatttaat
     1501 atctttcaaa aatagaagaa gttactaatt ttgcgccatg aaaattaccg ccgttgtcgt
     1561 gacttttaac cggttggggc ttttaaagga gtgtatcgcc ggtttgaaac ggcaaacagc
     1621 ccccttacac gagatcgtcg tcgtcaacaa tggcagttcg gatggtacgg atgtctggct
     1681 ggccggagaa caagggctca ccgtcgtcac ccagggaaac cttggcggat cgggcggtca
     1741 gtacaccggc atcagcaagg cgatggaaag ggatcccgac tgggtctggc tgatggacga
     1801 cgacgcggag ccctatcccg atgcattgga gaagctgctg cattttctcg acgcggatac
     1861 cgtagccgcc gccgcctgtt ccgttatcga taatgcaaac cggatctcgc tggtacatcg
     1921 gggggagtat gactatcgcc ggctgaccag cgagtatggc tgccggccga ccccggaaac
     1981 ggaatacgcc agggactcgg tcgatgtcgg atacgccacc tttgtgggga tcatcgtcaa
     2041 cagccgggcc gtgaagacca tcggcctgcc caaaaaagag ctcttccttc actttgatga
     2101 tatcgaatac tccctgcgcc tcaggaaatt cggtcggatc gtcctcgtgc cggccagcag
     2161 gatgctccat aaagagaacg ccagtacgca tttctatacg aagaaggtcc tcggtaaacc
     2221 acgggtcaga gtgaaatacg agaaactctg gttacggtat ttcgtcatcc ggaacgtcat
     2281 ctggggtgtg aaagaatacc atgggaagaa atgggatacc cccttcgtcc tggccaacta
     2341 ttatatccgg agcgtagtgg gtatcctgct gtttgacgat cacaaatgga aaaggatcgt
     2401 atttctcacc ggcgcattca gagacgggct gtccgcccac ttcgagaaca gctatgacta
     2461 tatcaatggc cggacagggc tttacccata aaacgatgcc tgccggagaa cacgcctgcc
     2521 cgggccttat ttcctcagaa taaagatcgc ctgatcccct ttgccggtct tgttggccac
     2581 aaaggttttc ctccggtatt cggccatttt ttcaggactg atggcattct tttgtttaat
     2641 caggggaatg ccctggcggt actgctcact gatataaaat tgttttggcg tgctgtcgta
     2701 gacgatgcca tccatgcgga accccaccgg accgcaaagc cgttccatgg tatccaccga
     2761 cgggatgaag atatgacgcg gggcgtctat ctggaaccag ttctcccggt attcctccca
     2821 ggccatattg gggatgaccg gtacgcgaat gacacaataa ccgccgggta ccaaccggtc
     2881 atacaccatc ttcaacgtct cctgcggatt gggaagatgc tcgaatgtat gctgcagcgt
     2941 gatcagatcg aaatccttct cttcatagtc cgccaggtat tttctttgga tcgtgacctt
     3001 tcccgaatgg acgatctcct ccttgagaaa ggggtcgatc cccctgagat cggtaaaacc
     3061 cagggcctcg agacggtgca ggtaaaaccc catcccggaa ccgacatcca ggatacggga
     3121 gtgtttgtgg atgcccccga gataggagat aaagcggatc tcttcccttc cggggacaaa
     3181 aagcgcgatg atcctgccga ggaaatgttc attgaacaga tcggccttat cccgccattc
     3241 ggccatccgg gtggacagcc gcttcagcag actcttgtcg ggggaatggc tgtaataggc
     3301 ggcgccgtaa tacttcccca tatcttcggg aaaagcgact atctgcagac aattgcacac
     3361 gggacaaagg aaatactcga aatattcgtc caggccgaac atcatttctt tgacccggta
     3421 aacatccttt atccccttat catttttaca aattctgcat tcaggcattg gttagcttat
     3481 tttttgaaat tgacggcaat ttaatatcaa attgcgggaa tacattggta aaagccagga
     3541 tccagatcac atattccgcc gtactggtga taaaggaacc ggagaactgg taaaggaaca
     3601 tgaacaggaa aagggccagc cggtaataat tcttccagac ctttgtatat acgaacaagc
     3661 ctatttcaat gccgagccgt gcaaaaagcc ctatccagcc aaagacgacc agggtctcgg
     3721 ccatggcatt ggggatggtg atgatatcga aatcctggtc ataagagtaa taatcccgga
     3781 taatatcggc ccctatcagc ttcagttgcc ccgggccgat accacaccag gcgttccgcc
     3841 gttcgaggat acgctgagcc aggtagaagg cgtcggtggt gcgcccctgc gttgaaatat
     3901 cacgacccga gaacacattt tccgtccgga cgacaagggc attctccgga aagaatacca
     3961 ccagcacgag cacgccggcg atcatcagca cgacgccggc gatcaccagg tgaaagagcc
     4021 tttttttccg ggtcagtgga ccgaaataaa gcaggtaggt gagcccgcag gccgcgcaaa
     4081 gacaaatgat cactcccatg gaaagcgaga gcaggtaggg tacggtcagc aacagggcca
     4141 tccccaggag accccgctta ttttgcttta acatcacctg gtagaagaag tacaggaaca
     4201 ccggggtgaa catcgtggca taatacgagg cttcataggt gaacagtttg aagcgcttca
     4261 ggctgttgac gccttccgta tatacctgtt cgatccagaa gatatcggcc cagggcgtaa
     4321 aatagaaagg gatggcgatg aggcaaaagg caaaattgac gaccaggatc cgccaaaaga
     4381 tcctctcttt gtcctggcag gtcttcagga acgtatagac ggcctgggcg aagatataca
     4441 cgcccatcat attcgcccag gagaccagga atttccccat atccatgccg gtaaaggccc
     4501 agccggcaaa gaacagcgcc gaaaccgcca ggaaggggaa caggacctcc tttctgcggg
     4561 tcctgaccac ccagatatac agcagcgggc tcagcagggt agtatagagc agcccccagg
     4621 gaagccccac ggagttaatg aagaaataaa caaatacgaa ggggaagtat ctatttattt
     4681 tcattggatc gcatttttaa gaaagatcgg tagcctttgt aaactcccca ggcccttttc
     4741 catttgcccg tcgacacccg tttcaggaac cggagaaagg taaggctcat cgctaatccc
     4801 gaaggatctt ttctgaattt ggataagaac agcagtccgt tccgggtata gaaataatcg
     4861 gccatgaaac ccctgccgat ggtggtgctt atcttgtcat aacagatggc gtcggggcag
     4921 acctggagca gatagcccct ttctttcgcc cggctgcacc agtccgtttc ctcccagtag
     4981 aggaagtatt cctccggcag caggccgatc tcgttgaaat gagcggtatg cgtaaaaaga
     5041 gcgccccccg agatataatc cagccgcggg agatcggcct cttcctgcag cagctcgacc
     5101 gtacccgacc gccaattgat actgcccccg ccgtacaaat ggatggcatg cggcggatcg
     5161 aagtatttta tcaccgaccc ggtgatcgcc ctgaacggcc ggcttccggc aaaccccacc
     5221 agggtcgaaa gcgtattctc ctccaccgtc atatcggggt tcagcagcca gacataggcc
     5281 ttctccgtca acagggcgcg gatggcgaca ttattgccct cggcaaagcc cttattgcga
     5341 tcgttctgca acagcacgag gcggggcagc tccgcgggga cgatcccttc ctgttcccat
     5401 tcggcgcgct ggaacaaccg gtagtccgtc cggccttcgg cccaggccgc gaggctttcg
     5461 agggagccgt tgccggaagc attgtccgtt acgatcacgg taaatggctg atgggtgctg
     5521 cggaacaggg actccaggca gtcccgggta tccgtccatt ttttgtagtt gacaataatg
     5581 atgtatacgt tgttagccat ttattgctgc ttcggggaat aaggaacgga cactggtgag
     5641 agcggccgcc acgatctggt gcatgtcgta atatctgtat tccgcaagcc ggccgccaaa
     5701 aagaacattc tcttcggcat ccgccagcaa ccggtattgt ttaaagatcc gggtattctt
     5761 atcgtcgttg acgggataat acggttcgag cccgggccgc cattccaggg ggtactccct
     5821 gctgataacc gtgtgctcct gggtgccgaa ctcaaaatgc ttgtgttcga tgatccgggt
     5881 atagggaata tctctctccg tataattgac gacggcattg ccctggtagt tgggcaggtt
     5941 gagccgttcg tgttcgaacc ggagcgaccg gtattccagc tgtccgaacc ggtaatcgaa
     6001 gtattcgtct atctggcccg tgaaaacgat ggtatgcgcc agcggggcaa gcgcattccg
     6061 ttcgcggcag aagtctacac ccagcctgac ctcgcagccc tcgagaagcg ctcccgtaag
     6121 ccggttatac cccccgatgg ggataccctg gtatttatcg ctgaaataat tgttgtcgaa
     6181 ggtgaaccgc accggcagac ggcggatgat aaaggcgggc agttccgtgc atttgcggcc
     6241 ccattgcttt tcggtatagc cctttatcag tttctcatag atatcatgcc ctacgaggga
     6301 aagggcctgc tcttccaggt tggcgggttc ttttgtctgc cattgcccct tctgctcctc
     6361 gatcttcgcc ctggcctcct ccggcgttct taccttccag agctggtaga aggtattcat
     6421 gttgaacggg agattgtaca attcgccttt ataacaggcc aggggcgaat tggtataccg
     6481 gttgaactcg acaaaagaat tcacatattt ccacacccgc tcgtcattgg tatgaaagat
     6541 atgcgccccg tatttatgca cattgatccc ctcctcatcc tcgcaatagg tgttgccgcc
     6601 gatatgggga cggcgatcga ctaccaggca ggtcttgccc cgcttcctcg cttcatgtgc
     6661 aactacggcg ccgaataacc cggcgcccac aataagaaaa tcatacttat ccataccgga
     6721 cttttgtgag tagatctgcg atcgtttgtt ttacaaatat gttccgaaag acaaaatact
     6781 gggtacaaaa atagaccgtc gccgccatcg atatcaccaa cagcatccgc aacagccctt
     6841 ccgcaaccaa taaaccggca agatagtaac aaggatagaa aagaagacag gccagcccgc
     6901 tttgccacag gatattatag ggcagcgcca cccggaaatg ccggagggca taccagccgg
     6961 tgacgatcgt aaccaatcct tcggtcagca gggtcgtata ggccgtcccc atatacgaga
     7021 accgcggtat caggatcagg ttgcacacga cgctcaggat catgcccagc gtaacggcat
     7081 aaagggtaag cttctccttt cccatggagg tcaggatctg gatcccgaac aggttgctca
     7141 gcccgatgat cagggtgagg ggcgccatcg tccggaccac cgggatcgcc ggaagatagt
     7201 tgcgattggc gaggacggta atgagttccg gcgccagcag cagcagcccc gcgccgatgg
     7261 gcagggagat caggatgata taggcaaagg atttttgtat caggttttgc acatattccc
     7321 ggttcccgga agataaggag gccgagatgc gggggatgag ggccgtgctg agcgccgtca
     7381 taaaggcaag ggggatctta cagatcttga tcgccgtagc ataatacccc accgcggtgt
     7441 cgtccgcgag aaaaccaaga atgaccgtat cgagcagaat atagacgctg atcgccacat
     7501 tggaaaagaa gatatgcaac agcggccgca tatgccgcat acaggaaagc ccggtgaatt
     7561 caagccgcac ctcgcggcgg gcataggaga aattgatcac gccgttgagg aaggcgctga
     7621 taaaggtgag gccataatac agcagcaggt cctccttctt ttttaccagg acaaagaccg
     7681 cgatcaggaa aagaaagcga accgagatgg tccgcatggt aatatacttg aaccgttcga
     7741 tcccctggaa gaaccattcg acggtgaagg cattcatcaa tagggtaccg cagctgagaa
     7801 aatacaggag cttatgctcc gccagttttt cgacggtgaa aataagcccg atgaaaacga
     7861 cggtgatgaa caacgagata acgagctgga tcagtaccag ttcggagaat agcctggcca
     7921 ggcgtgcggg gtcatgccgc accttggccg tttctatcac cccatagacc gggatgccga
     7981 tggccgcaaa agagatgaga tattgggtga gactgtcggc gaaactgacc aggccgatac
     8041 ccggaggctc gaggattttg aacgtataag gaaagatgat taacgggaac aatatctgac
     8101 ttacggaaag cagagaattg tatatcaaat ttcttttaat cactcgcgaa attaatcata
     8161 atgagcatat ttcgtctaaa aacttgggcc cctgaatgga aatattgatt accttgtggc
     8221 attttttgca accagttttt tttacataaa cggccggaat aatattcaat aatattatta
     8281 tatatgagcg acagcccttc gttccaaaaa gacacgaccg atgatctgga tcttgccccc
     8341 atgctggtga aggctatcac ctttatccgt caacatggct ggaagatcat gttgagcagc
     8401 ctggtgggat tggtcatggg cgggtttctt tactatctgt tgccgcacac ctataaagcc
     8461 cggatggtcg ttcagtccag cgtgctttcc aacgcacagg acctgcgcgt catcaacaac
     8521 tgggatgatc ttttgaacaa gaacggctat gcgatactgg gcgcgatctg gcaccagccg
     8581 tacaatgtgg tgtccgaatt gaacggcatc aaggcagaga tcatccccgg taccgacgag
     8641 aacagcggca tcgtgatcga ggctgctgtc agcgactcct ccatgctgcc gggagtccag
     8701 caggggatca tctatggatt ggagaacagc gagtatatca aacagcgggt cgccacccgc
     8761 agacagaacc tggaatacca gatcgcacaa acaaagcagg aactggctaa gctggattcg
     8821 gcaagatcct atctccggag tttttccggc ggaagtgaac ccgcgaattc ccggcttgta
     8881 ctcgatgttt cgaacgtcac cagtcagcgg ctgagctgtt atgaccgcat cgccggcttc
     8941 gaagagaaac tgaagttcac gcgtggggtc tacctcctcc aggagttcgt gccggccaaa
     9001 cggggcaaga tcatcccggc gcccgtgctg cccctgctgg ggtttgccct gggtctcctg
     9061 ctcagctatt gctcggtggt ct
//
LOCUS       DATGFG010000627         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_688267, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000627 DATGFG010000000
VERSION     DATGFG010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688267"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VKQ52_11760"
     CDS             <1..398
                     /locus_tag="VKQ52_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87915.1"
                     /translation="ATAALLGNRLLNKPTGAGYTENPPLHPNELTPHNLAFFAACQYS
                     WILGCNPYSMCFLYGWGKNNVPYMHSNYGHGSQRGGISNGITGEKDHGDGSGIEFLTS
                     DNGNEWRWTEQWLPHAAWFLDAVTAMNRP"
     gene            complement(775..1533)
                     /locus_tag="VKQ52_11765"
     CDS             complement(775..1533)
                     /locus_tag="VKQ52_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HLZ87916.1"
                     /translation="MKITITKNEQEFDVTAAWRIIGQMLAKPDAVIGLSTGQTTIGMH
                     RIVAEIYGKYPFDVSRVTLFNVDELTNLPREYAGSCYSMIHTQLAAPLGISDENFIMP
                     PTMSDDFVREARLFEERLAARGGADLQMLGIGWNGHIGINQPGTPFGSETWVSPMDPI
                     FEAKVRKETGLPPEHELGGLTLGIKNIMQTRKIVLIAKGEHKASIIEQAILGPVTTDI
                     PASVLQLHPNCEILLDAAAGARIAKTAWERGFEW"
     gene            complement(1535..1801)
                     /locus_tag="VKQ52_11770"
     CDS             complement(1535..1801)
                     /locus_tag="VKQ52_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HLZ87917.1"
                     /translation="MITKEYLILAPEGMHARPATAMVKLARQFQVAIQLRKGGKAARL
                     NSLMSILALGIKGGEAVVLEFEGADETAAVVAFDGFFADNLKDW"
     gene            complement(1798..>2240)
                     /locus_tag="VKQ52_11775"
     CDS             complement(1798..>2240)
                     /locus_tag="VKQ52_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HLZ87918.1"
                     /translation="SVEELKAAKAVVAEAKAELTRAGIVFAAEVPVGIMIETPSAALT
                     ADLLAKEADFFSIGTNDLCQYTLAVDRMNEKIASLYDPFHPAVLRLIRMVIEEGHRAG
                     IPVGMCGELAGDAAATKILLDMGLREFSMNAASIPEVKNIIIGL"
BASE COUNT          472 a          792 c          507 g          469 t
ORIGIN      
        1 tggctaccgc cgccctcctc ggcaaccgcc tgctgaacaa gcccaccgga gccggctata
       61 ccgaaaaccc gccgctgcat cccaacgagc tcactccgca taacctcgcc ttcttcgccg
      121 cctgtcaata ttcctggatc ctgggctgta atccctattc gatgtgcttc ctctatggct
      181 ggggaaagaa taatgtaccc tatatgcatt ccaactatgg ccatggctcc caacgcggcg
      241 ggatctccaa tggcatcacc ggcgaaaaag accatggcga cggcagcgga atcgaatttc
      301 tcaccagcga caacggcaac gaatggcgct ggaccgaaca atggctcccc cacgccgcct
      361 ggtttctgga cgccgtcacc gccatgaacc ggccataacg ccgcccccgc ccctgccgcc
      421 ctatcgcgcg ccaatcctgc aaaacaacac ggctaaatcg gtttagcggc cgctcaatct
      481 gggaaagacc gcctgccgga tgcagcgggg attccaaaag cgggaatctg gggtccggga
      541 cccccgttga caaccaacac cttatgcggt ggcacgccac ttgtctctta tataggcatg
      601 aaaaagctta tcaaagagaa tcatttatcg gtaccgggtc ttatcatcgc cttcctcctc
      661 ggaatgctgg cgaccttctg cttctacatt ttcaggtaac ggccggcccg cggactcctg
      721 cccgcaggta acagcccggt ccgcagataa cagccccgcc ccgaccccta aaccctacca
      781 ctcaaaaccc ctttcccagg ccgttttcgc tatccgcgcc ccggccgccg catccagcag
      841 gatctcacag ttgggatgca attgaagcac agacgccgga atatccgtcg tcaccggccc
      901 caggatcgcc tgctcgatga tcgacgcctt gtgttcccct tttgcgatca gcacgatctt
      961 cctcgtctgc atgatatttt tgatcccaag cgtcagtccg cccagctcat gctcgggcgg
     1021 caagcccgtc tcctttctta ccttcgcctc gaatataggg tccatcggcg agacccaggt
     1081 ctcactcccg aaaggcgttc ccggctgatt gatcccgata tgtccgttcc aaccgatgcc
     1141 cagcatctgc agatcggccc cgcctctcgc cgccagccgc tcttcgaaca gccgcgcctc
     1201 gcgaacaaaa tcatcggaca tcgtcggcgg catgataaaa ttttcgtcgg atatccccag
     1261 cggtgccgcc aactgcgtat ggatcatcga ataacaactc cccgcatact cccgcggcag
     1321 attggtcagc tcatcgacat taaaaagcgt gacccgcgag acgtcaaacg ggtatttccc
     1381 atagatctcg gcaacgatgc ggtgcatccc gatcgtcgtc tgacccgtcg acaatccgat
     1441 cacggcgtcc ggcttcgcca gcatctgccc gatgatccgc caggcggccg tcacatcaaa
     1501 ttcctgctcg ttctttgtga tggttatttt catgttacca gtcttttaaa ttgtcagcaa
     1561 aaaagccgtc aaaggccaca actgctgccg tctcatcggc cccctcgaat tccagcacca
     1621 ccgcctcgcc acccttgatc cccagcgcaa ggatactcat caggctgttc agccgcgccg
     1681 ccttcccgcc cttcctgagt tggatggcca cctggaattg ccgggctaat ttgaccattg
     1741 ccgtggccgg ccgcgcatgc atgccttccg gcgctaagat caggtattct tttgtgatca
     1801 taatccaata ataatattct tcacctccgg tatcgacgcc gcattcatcg aaaactcccg
     1861 caggcccatg tcaagtagta ttttcgtcgc cgccgcgtcc cccgccaatt ccccacacat
     1921 ccccaccggg atgcccgccc gatgcccttc ttcgatcacc atccggatca gccgtaacac
     1981 ggccgggtga aaaggatcat acaaagaggc gatcttctca ttcatccggt ctacagccaa
     2041 cgtatattgg caaagatcgt ttgttccgat actaaaaaaa tccgcctcct tcgccagcag
     2101 atcggccgtc aaggccgccg acggggtctc gatcatgatc cccaccggca cctccgcggc
     2161 aaagacgata cccgccctcg tcaactccgc cttcgcctcg gccaccaccg ccttcgccgc
     2221 cttcaattcc tccacactcc
//
LOCUS       DATGFG010000628         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_953788, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000628 DATGFG010000000
VERSION     DATGFG010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953788"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2142
                     /locus_tag="VKQ52_11780"
     CDS             <1..2142
                     /locus_tag="VKQ52_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ87919.1"
                     /translation="NTSFSLFDDHVRIGENLFAFYKTNPGYLNAPGVNSTNSINAAYQ
                     IPNIIPIYDIAGNYAGTISQGTGNSSNPVAIQQRQANNTNQDYQIVGNVFADVDFLDH
                     FTVHTSVGGTADSYYYNGFATTPYENAENNTAANLYEETFGWNSSLIWTNTLKYANKI
                     GKHDLSILGGCEYIYNTGKAVLATRGNYYITDSSNLTVSPNLWTLNFGQASTQTNNSG
                     VIGDNGILTPYQIAIFSLLGRLDYSYDGKYLLSATFRRDGSSVFAPSQRYGNFPAVTA
                     GWRISQESFMSGIHWLNELKIRGGWGKLGSISNINPTNAFTLFGQQINQSYYDLNGTS
                     SNPVAGLYVSQYGNPNTSWEKDQLTNIGFDASVLGNKLDFSFDWYKKLISGLLFVPQV
                     PGTNGGAVDPFTNSGNVQNTGVDLALTYHGSVHNDLRFDITGTFTSYNNKVVALPPGT
                     LYIDEPAGAQTITSRIQPGHPLGAFFGYRVIGLFQSWTDVYKSPTQQDAAPGRLKYAD
                     VDHSGKIDPKDRTFFGNPNPKFSAGLNLSVNYRKWDLYMFLYASVGNDILNNVKSSTD
                     FPQSFGNQISTSVAVNSARLVNASGAPAGINDSTAHVANPGTNVPMLEQSANFSNSGV
                     FNSYIMEKGSFLRCRNLTIGYSFVSNAIKRLHCDKLRVYAQALNLFTITKYSGLDPEL
                     NPGSNVVFGVDSGVYPNNQKTYNVGVSLTVH"
     gene            2167..3999
                     /locus_tag="VKQ52_11785"
     CDS             2167..3999
                     /locus_tag="VKQ52_11785"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ87920.1"
                     /translation="MTKKVKRIAVVTMVLLTIIIYACNRKLDLQPKGSLLASNVTNKS
                     GVEGLLIGAYALLSGENVPGQNLGNGSGASNYVYGSECADDSYKGSTPSDQPDAIPLM
                     TWSLAQAGTSSYLDEKWIVLFTGIQRANDVIRTLRLSPSISPADTVELEAEARFLRGF
                     YHFEGKKIWNNFPYAGENVSYTNNNLDVPNFTGGGYVNIWPQIVGDLAYAANNLPGTQ
                     PNVGRVNKWAAMAFLAKAYLYQHRYDSALALLNQVIASGTTPSGTPYSLEPVYESNFN
                     PAQKNSSESVFATQASVNDGSSIATNGGNGDTGDELNFPYNHGPGCCGFNNPSWNLVQ
                     AYKTDANGLPYLNGSYNSAPLVSDRTNPWTGTIDPRLDWVAGRPGEPYFDWGTIDSTW
                     IRDVLDDGLFVPKKNGYAQSQQGKFSSTETVFWGAAEVDANNVNLCRFADVLLWAAEC
                     EAQIGSLDRAETYVNRVRQRAANPQGFVYLNATYDAGSSTYSPQTTPGDKYLVKPYAA
                     GAFSAQGQGYAISAIQMERRLELAMEGQRFFDLSRWDNGTGSMQATLNAYVAAEKNRS
                     GFYRVNSGANFTRGVNEYFAIPQNELDAENAKGTVYLKQNPGYQ"
     gene            4069..>5301
                     /locus_tag="VKQ52_11790"
     CDS             4069..>5301
                     /locus_tag="VKQ52_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87921.1"
                     /translation="MLKSYLTTAWRNIRKNKMFSTINVVGLSIGMAACLLILQYVNFE
                     LSYDRFDTNIGDLYRVYNDRYQNGKLIQHGTITYSGIGKAMQHDFPEVMDHARVEPFG
                     KQIVIYGNKKFGDQQMFGVDNSFLTMFSYHLVAGDPNTALVKPYTAVLTETAVNRYFG
                     LKGNDVASALGKVIFVDRDSIPYKVTGICRDVPGNSHLQFDLLTSYVTLYSGKEPWKE
                     SDSDFRDSDFWHYIQLKHGTDYKALEAKFGGFSQRYFQGDKVSGSVEKFYLQPLARAH
                     LYSDFEYEIGKTGSATVVWGLLIIALFIIVIAWVNYINLSTARSTERAKEVGVRKVIG
                     AAKSQLIRQFLTESFLINLFALILAIALMFIFQPAFNALIGHKLSFSYLLQRSVGGYS
                     ITVGLLVLTLTGIFVSGFY"
BASE COUNT         1299 a         1505 c         1341 g         1156 t
ORIGIN      
        1 aacaccagct tcagtttgtt cgatgaccat gtccgcatcg gggagaatct ttttgcgttc
       61 tataagacca atcccggcta tctcaacgcg cccggcgtca acagcacaaa ctcgatcaat
      121 gcggcctacc agatccccaa cattatcccg atctatgata tcgcaggcaa ctatgcgggc
      181 accatttcgc aaggtaccgg caactcctcc aacccggtag ccatccagca acgtcaggcg
      241 aacaatacca accaggacta tcagatcgtc ggaaatgtat tcgccgatgt cgatttcctg
      301 gatcacttca ctgtccatac cagcgtcggc ggaacggccg attcctacta ttataatgga
      361 ttcgccacca cgccgtatga aaatgcggaa aataatacag ccgccaacct gtatgaagaa
      421 accttcggct ggaacagcag cctcatctgg accaacacct tgaaatacgc caacaaaata
      481 ggcaaacacg atctgagcat cctcggcggt tgcgaataca tctacaatac cggcaaagcc
      541 gtcctggcaa cccgcggcaa ctattatatc accgactcaa gcaatctgac ggtaagcccc
      601 aatctttgga cgctgaactt cggccaggct tccacccaaa ccaataactc cggcgtcatc
      661 ggtgacaatg gcatcctgac cccatatcaa atagccatat tctccctgct cggcagactg
      721 gattatagtt acgacggcaa ataccttctc agcgccactt tccggcggga tggttcttcg
      781 gtattcgccc cttcccagcg atacggtaat tttcccgccg ttactgccgg atggcggatc
      841 tcgcaggaat cattcatgag cggcatccat tggctcaatg aactcaagat ccgtggaggt
      901 tggggcaaac tgggatccat cagcaatatc aacccgacga atgccttcac cttattcggg
      961 cagcagataa accaatccta ttatgatctc aatggaacga gcagcaaccc tgtagcaggc
     1021 ctttatgtga gccagtatgg caacccgaac accagctggg agaaggacca gctcaccaac
     1081 atcgggttcg acgcaagcgt gctcggcaat aagctggact ttagttttga ttggtataag
     1141 aaactgatca gcggactgtt gttcgttccg caggttccag ggacgaatgg aggcgccgtc
     1201 gatcctttca ccaattcggg taatgtacag aacacgggcg tggatctggc gctcacctat
     1261 cacgggtctg ttcacaatga cctgagattt gatatcaccg gaacctttac ttcttacaac
     1321 aacaaggtcg tcgcgcttcc tcccggcacg ctgtacatcg atgaacccgc cggtgcgcag
     1381 acgataacct cgaggatcca gcccgggcat ccgcttggcg catttttcgg ctaccgggtg
     1441 atcggcctct tccaaagctg gaccgacgtg tataagtcgc ccacccagca ggatgcggcg
     1501 cccggccggt tgaagtacgc cgacgtggat cacagcggga agatcgatcc caaagacagg
     1561 acattcttcg gcaaccccaa cccgaaattc agcgccggcc tcaatctctc cgtgaattac
     1621 aggaaatggg atctctacat gttcctgtac gcttccgtcg gcaacgatat cctgaataat
     1681 gtgaagtcgt ccaccgattt cccccagtct ttcggcaacc agatcagcac cagtgtggcc
     1741 gtcaattcgg caagactggt gaatgccagc ggcgcacccg ccggcatcaa cgactcgaca
     1801 gcccatgtcg ccaacccggg aaccaatgtc ccgatgctcg agcaaagcgc caatttcagc
     1861 aattcgggcg tattcaattc ctatatcatg gagaagggct ccttcctccg gtgcagaaac
     1921 ctgaccatcg gctacagttt cgtttccaat gcgatcaaac ggctgcattg cgacaagctg
     1981 agggtttatg cacaggcgtt gaacctgttt acgatcacca aatattcggg actggatccg
     2041 gaactgaatc cggggagcaa tgtcgtgttt ggggtggatt cgggcgtata tccgaataat
     2101 caaaagacct ataacgtggg ggtaagcctt actgtacatt aattcatttt gaaaaaagga
     2161 atatatatga caaaaaaagt aaaaaggatc gcggtggtaa cgatggtgct gctgacgatc
     2221 atcatatatg cttgcaatag aaaattggac ctccagccca agggttcgct gctggcttcg
     2281 aatgttacca ataaatccgg ggtagagggg ctgttgatcg gtgcttacgc gctcttatcc
     2341 ggcgagaacg tacccgggca gaatttgggg aatgggagcg gcgcctccaa ctatgtatat
     2401 ggaagtgaat gtgccgacga ctcctacaag ggatccacgc cctccgatca gcccgatgcc
     2461 atcccgctca tgacctggtc tctggcacag gccggcacca gtagttatct ggatgagaaa
     2521 tggatcgtcc tgttcacggg tattcagcgg gcaaatgatg tgatccgcac ccttcggctg
     2581 tccccatcca tttcgccggc ggatacggtg gaattggagg ccgaggcaag gttcttacgc
     2641 gggttctatc atttcgaagg caagaagatc tggaataact tcccttatgc aggggagaac
     2701 gtttcgtata ccaataacaa cctggatgtc ccgaatttca ccggcggagg gtatgtcaat
     2761 atctggccgc agatagtggg cgacctcgcc tacgcggcca ataatttgcc gggaacgcaa
     2821 cccaatgtcg ggcgggtgaa caaatgggca gccatggcct ttttggcgaa ggcttacctg
     2881 taccagcatc ggtatgatag cgcgctggcc ttgttgaacc aggtcattgc cagtggcacc
     2941 actcccagtg gtactcccta ttccctcgaa cctgtttacg aaagcaattt caaccccgcc
     3001 caaaagaata gctccgaaag cgtttttgcc acccaggctt ccgtgaatga cggttcgtcc
     3061 atcgccacca atggggggaa cggcgatacg ggggatgagc tgaacttccc ctacaaccat
     3121 ggacccggct gttgtggatt caacaaccct tcatggaatc tggtgcaggc gtacaagacc
     3181 gatgccaacg gccttcctta cctgaacgga agctataaca gtgctccgct ggtaagcgac
     3241 cggaccaacc cctggaccgg caccatcgac ccccggctcg attgggtggc gggcagaccg
     3301 ggtgagcctt atttcgattg ggggaccatc gattccacct ggatccgcga cgtcctcgat
     3361 gacgggttgt tcgtccccaa gaagaacggc tatgctcaga gccagcaggg aaaattctct
     3421 tctaccgaga cggtattctg gggcgccgcc gaagtcgatg cgaataatgt caatctctgc
     3481 cgtttcgccg atgtccttct ctgggccgcc gaatgcgagg cgcaaatcgg tagtctcgac
     3541 cgggccgaga cctacgtcaa tagggtgcgt caaagggccg ccaacccgca gggattcgtc
     3601 tatttgaacg ctacgtatga cgccggttca agcacctact ccccgcaaac gactccgggg
     3661 gacaaatatt tggtaaaacc ctatgccgcg ggcgcattca gcgctcaggg ccaggggtat
     3721 gcgataagcg ccattcagat ggaaagaaga ttggagctgg cgatggaagg ccagcgcttc
     3781 ttcgatctgt ccagatggga caacggcacg ggctccatgc aggctacctt gaatgcgtat
     3841 gtcgccgcag aaaaaaacag gagcggcttc taccgggtca acagtggcgc caacttcacc
     3901 aggggcgtca acgaatattt tgccatcccg cagaatgagc tggacgccga aaacgcaaag
     3961 ggaacagtgt acctcaaaca aaatccgggg tatcagtaag gcgaccgtcc gccccatatg
     4021 caacattttg ggggttggcc cgtctttcct ttgccaatcc ccaaaactat gctcaaaagc
     4081 tatcttacca ccgcctggcg caacatccgg aagaacaaga tgttctccac catcaacgtc
     4141 gtcggtctct ccatcggcat ggccgcctgc ctgctgatcc tgcaatacgt caatttcgag
     4201 ctcagctatg accggtttga caccaacatc ggggacctgt accgtgttta caacgaccga
     4261 taccagaatg gcaagctcat ccagcatggg accatcacct actccggcat cggcaaggcc
     4321 atgcagcacg atttccccga agtgatggat cacgcccgcg tggagccgtt tggaaaacag
     4381 atcgttatct acgggaacaa aaagtttggc gaccagcaga tgtttggggt agacaattcc
     4441 tttctcacca tgttctccta tcatcttgtt gcgggggacc cgaatacggc cctggtaaag
     4501 ccctatacgg ccgttctcac ggagacggcc gtaaacaggt atttcggatt gaaagggaat
     4561 gacgtggcat ccgcgctggg caaggtgatc ttcgtagaca gggactccat cccctataag
     4621 gtgaccggca tttgccggga tgtgcccggg aattcgcacc tgcaattcga cctcctcacc
     4681 tcttatgtca cactttattc aggaaaagag ccctggaagg aatccgactc cgatttcagg
     4741 gactccgatt tctggcacta tatccaatta aaacacggca cggactacaa agccctcgaa
     4801 gcaaaattcg gcggctttag ccagcggtat tttcagggcg acaaggtctc cggcagcgtc
     4861 gaaaaattct atctccagcc tcttgcaagg gcacatctct actcggactt cgaatacgag
     4921 atcgggaaaa cgggtagcgc gaccgtcgtc tggggactgc tgatcatcgc cctcttcatc
     4981 atcgtcatcg cctgggtgaa ttatatcaac ctctcaaccg cccggtccac cgaaagggca
     5041 aaagaagtgg gcgttcgaaa ggtgatcggc gccgcaaaaa gccagctgat ccgccaattc
     5101 ctcaccgaat cgtttttgat caacctcttt gcgctgatat tggccatcgc cctgatgttc
     5161 atctttcagc cggcattcaa tgcgctgatc ggccacaaac tctctttttc ctatctcctg
     5221 caaagaagcg tcggagggta cagcattacc gtcggcctgc tggtcctgac gctgaccgga
     5281 attttcgtat ccggttttta c
//
LOCUS       DATGFG010000629         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1408815, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000629 DATGFG010000000
VERSION     DATGFG010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408815"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VKQ52_11795"
     CDS             complement(<1..942)
                     /locus_tag="VKQ52_11795"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ87922.1"
                     /translation="MSRKHLFFLTLLAGSQAAFAQNPADTAGKQLDQVVVTATKYPVK
                     QSLTGKVLDIITREQLEKSAGKQLTEVLNTQAGVVVAGAQNNLGSQQSIYLQGADAGK
                     TLILIDGIPAYDPSGTNTAFDLNLINTDQVERVEILKGSQSTLYGSDAVAGVINIITR
                     KGAGKALTASGTAAGGSYGTFKGSAGIDGKIGNTGYNVQYTYLRSDGVSSAYDTTGKG
                     HFDHDGFNENIVLANLTQKVSDHFGLRGNFQYSYYTAGLDAGTYTDDKAYTSWDKNTR
                     AGIGGDYTIGAAALHFNYNYNTVTRHYLDDSSALISQG"
     regulatory      complement(1052..1276)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1339..2271
                     /locus_tag="VKQ52_11800"
     CDS             1339..2271
                     /locus_tag="VKQ52_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLZ87923.1"
                     /translation="MRQDHFYEYWPIIDRPDIEWPGGKRLAVYIGLNVEHYETGKPSI
                     GILDKAAGRDPDPLNEGWRDYGSRVGIWRIMELLDKYGIRPTVLLNSDVCKYYPRIIE
                     EGSRRNWSWVAHGRNNSMFAGGKQPQLPLEVERQYLNEILSTIQIATGKSCKGWLGPL
                     GLSETYDTVDLLADEGITYVLDWVNDDQPYPLKAFHGEIISVPYSLELNDLPLFLNHG
                     ITGPEFGQMIIDQFEVLQRDARKAARVMAIGIHPFVVGQPFRFKYFEQAIAFLSRQKD
                     VWITTSDEIAAWYMLNYYPTHVRKLREVGIAQGL"
     gene            complement(2239..>2700)
                     /locus_tag="VKQ52_11805"
     CDS             complement(2239..>2700)
                     /locus_tag="VKQ52_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87924.1"
                     /translation="LHRLAREMNKGVLVSTHELDLALQVADEVWLLQSAGPPHRGVPE
                     DLVLGGIFEAAFAKEDVSFDRATGVFRIHPEGVRPIGLAEKGITAFWTRRALQREGYT
                     VVSGQAVMAVDNIEIDEEQGRPVWVLRTRAGTRRLGSIAELLQALGDPHLP"
BASE COUNT          512 a          767 c          821 g          600 t
ORIGIN      
        1 gccctggctg atcagcgccg aggaatcgtc gagataatgc cgggtgacgg tgttgtaatt
       61 gtagttgaaa tgcagggcgg ccgcgccgat ggtatagtcg ccgccgatgc cggcccgggt
      121 gttcttatcc caggaagtgt aggccttgtc gtcggtgtat gtgcccgcgt ccaggccggc
      181 ggtgtaatag ctgtactgga aattgccgcg gagcccgaaa tgatcggaga ccttttgggt
      241 gaggttggcc aggacgatgt tctcgttgaa accgtcgtga tcgaaatggc ccttgcccgt
      301 ggtgtcatag gcggaggaga cgccgtcact gcgcagatag gtatactgga cattgtagcc
      361 cgtgttgccg atcttcccgt cgatgcccgc cgatcccttg aacgttccat agctgccccc
      421 ggcggcggtg cccgaggcgg taagggcttt gccggcgcct ttccgggtga tgatgttgat
      481 cacgcccgcc accgcgtcgc tgccatagag ggtggactgg cttcccttga ggatctcgac
      541 gcgttccacc tggtcggtat tgatcaggtt gagatcgaag gccgtatttg tccctgaagg
      601 gtcgtaggcc gggatgccgt cgatcaggat cagggtcttg cccgcgtcgg cgccctggag
      661 atagatgctc tgttgtgaac ccaggttgtt ctgggcgccc gcgacgacga caccggcctg
      721 ggtgttcagg acctccgtca gctgtttgcc ggcgcttttt tccagctgtt cccgggtgat
      781 gatatcgagt accttccccg tgagcgactg ttttacgggg tatttggtgg ccgttacgac
      841 cacctggtcc aattgtttgc cggcggtgtc cgcaggattc tgtgcaaagg ccgcctggct
      901 gcctgccagc agcgtcaaaa agaaaagatg ttttctgctc attacagttt gttttttgcc
      961 ggccgaaggg gaaataatgc cacatgggat caataaagac aggccgccat gaggcgcctt
     1021 caatcccgct tttcacccga aagccatgaa tgatgataga tcgatcgctg gcaggtcttc
     1081 tggcttctgc tctcaccggc ttaccttccc atccgcgggc ccacggacag tggtatgaag
     1141 tattgccggg tgattttccg ctgccggcag gcgccggcgg gcggaagagc atttacagct
     1201 acggggatag cgccggattc tcaccggtct tcccttttaa tgacgcgagg cgtcaaccat
     1261 gatcggcgcg aaagtacggt tttttgtgga ttcccggtcg ctgttccccg ggtataaaaa
     1321 ttgctgtgtc aaccgcatat gagacaagac cacttctacg agtactggcc gatcatcgac
     1381 cggccggaca tcgagtggcc ggggggcaag cggctggctg tctatatcgg ccttaatgta
     1441 gagcactatg aaaccggaaa gccctccatc ggcatcctgg acaaggcggc gggacgtgac
     1501 cccgaccctc tgaacgaggg ttggcgggac tatggcagcc gggtcggcat ctggcggatc
     1561 atggagctgc tggacaaata tggcatccgt cccaccgtcc tgctcaattc cgatgtttgt
     1621 aagtactacc cgcggatcat cgaggagggc agccggcgca actggagctg ggttgcccat
     1681 ggcaggaata attcgatgtt cgccggcggc aagcaacccc agttaccgct ggaggtagaa
     1741 cgtcaatatc tcaatgagat cctgtcgacg atacagatag ctacggggaa gtcctgcaag
     1801 ggctggctcg ggcccctggg gctgagcgag acctatgaca cggtcgatct gctggccgat
     1861 gaaggcatca cctatgtcct ggattgggtc aacgatgacc agccctatcc cctgaaggcc
     1921 tttcacgggg agatcatctc cgtcccctat tccctggaac taaatgatct gccgctgttc
     1981 ctgaaccatg ggatcacggg tcccgaattc ggacagatga tcatcgacca gttcgaggtc
     2041 cttcagcggg atgcccgcaa ggcagccagg gtcatggcca tcggtatcca tcccttcgtc
     2101 gtcggacagc ccttccgctt caaatacttc gagcaggcga ttgcctttct ttcccgccaa
     2161 aaagatgtct ggataaccac cagcgatgag atcgcggcct ggtatatgtt gaattattac
     2221 cccacccatg tccggaagtt aagggaggtg gggatcgccc agggcttgta gaagctccgc
     2281 gatggagcca agccgccgcg ttccggctct tgtacgcagg acccataccg gccggccctg
     2341 ttcttcgtca atttctatat tatcgacagc catgacggct tggccggaca ctacggtata
     2401 tccttcccgc tggagggccc gccgggtcca gaaggcggtg atgccctttt ccgccagccc
     2461 gataggccgc actccttcgg gatggatgcg gaacacgccg gtggcgcgat cgaagctgac
     2521 atcttccttt gcaaaggctg cttcgaagat gccgccgagg accaggtcct cgggcactcc
     2581 cctgtgcgga gggccggccg attgcagcag ccagacctcg tcggctacct gtaaggcgag
     2641 gtccagttca tgggtggaca ccagcactcc cttgttcatt tcccgcgcca ggcggtgaag
//
LOCUS       DATGFG010000630         4682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_229151, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000630 DATGFG010000000
VERSION     DATGFG010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229151"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VKQ52_11810"
     CDS             complement(<1..851)
                     /locus_tag="VKQ52_11810"
                     /inference="COORDINATES: protein motif:HMM:NF020928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HLZ87925.1"
                     /translation="MQAEGATIPFMARYRKEATGNMDEVQIADVVEQIKYFSELDKRK
                     ETVLKTIEGLGKLTPGLKERIDHCYDATVLEDIYLPYKPKRKTRATTAVEKGLEPLAK
                     FLFEQGAGDPAEEAGKFINEQVKDMKDALQGARDIIAEWIAENEQARNKVRQLFTESA
                     ALSAKVITGKKDEEEAQKYRDYFEFNEPLADCPSHRILAIRRGEKEGYLIMDLNIDKA
                     SAVDALDRIFVKGGPAAAEVTKAVEDSFDRLLKSSIENEFRLISKNKADEEAIRVFAE
                     NLRQLLLA"
     gene            979..2340
                     /locus_tag="VKQ52_11815"
     CDS             979..2340
                     /locus_tag="VKQ52_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLZ87926.1"
                     /translation="MDIKNNILETIGRTPLIRLNKLTRELPCTVLAKVEYFNPGNSIK
                     DRMALKMVEVAEQEGKLRPGGTIIECTSGNTGMGLALAAVVKGYKCIFTTTDKQSKEK
                     IDILKALGAEVIVCPTNVEPEDPRSYYSIARRLAKEIPNSFHANQYDNLANRLAHYES
                     TGPEIWEQTDGKITHLVVATGTGGTVTGTAMFLKEKNPAIQVWAMDPFGSLLTKYFRT
                     GQIDMGEVHPYISEGIGEDFVPQNYDMRVVDAFEQVTDKDAAIMARRIAREEGLFCGY
                     SAGTAVQGLMQLKDLLKKDHLVVVILHDHGSRYVGKVYNDQWMIERGFLDVKTFKDII
                     AGRGAKRLITATPENTVAEAFELMQKYHIENLPVIKDGEPIGAISESGLFSRVMNNGS
                     DIKGKTIAEVLEKPYPIVAFDTPLERLSTLITRENGAVLGKDDSGNYHIVTKYDVIHA
                     LGA"
     gene            2968..3741
                     /locus_tag="VKQ52_11820"
     CDS             2968..3741
                     /locus_tag="VKQ52_11820"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HLZ87927.1"
                     /translation="MPSFTDQLGRTIFLPRIPHRIISLVPSQTELLYTLGLDEAVVGI
                     TKFCVHPESWFRSKPRIGGTKAIDPARVDALQPDLIIANKEENEKPQIEALATRYPVW
                     ISDVHTLQDALAMIRDVGAITGKATEASALATTIATRFQQLPIPVKPVPAAYLIWRNP
                     WMAAGGDTFINDMLRYCGYTNVFSGHDRYPTLELSALAGPNCPHILLSSEPYPFRERH
                     ITEIKEVLPDATIRLVDGEFFSWYGSRLLEAPAYFRQLQ"
     gene            3763..>4682
                     /locus_tag="VKQ52_11825"
     CDS             3763..>4682
                     /locus_tag="VKQ52_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87928.1"
                     /translation="MQFSDQPTGSESNKPSRKKPIFPVNEGLRNYLKQHGREVKLPVS
                     YRDLLLHITYSVPLKDKHHQDTLWETALYDMRHWDFIREGLVQMYAILKTEGDLSYTK
                     HLDVARIDFCSFANSHPFRIRIVNKFNDNYDHYYIKQADASRIYGLELEHLLSPNRIT
                     FFTHHNTLVEEHIPGIPGDLFIQNFLEDPQTNKIRLAKEFVKFNERCFVRLLGDMRSY
                     NFVVDITPDIEDYQYRIRAIDFDQQSYEGRKNLYIPQFFKENRAFVDLCLNHLNKESI
                     EQYQTEERTMMAFRVASSRFRLMELLNIMA"
BASE COUNT         1201 a         1485 c         1015 g          981 t
ORIGIN      
        1 gccagcagca gctgccggag attctcggca aacacccgga tggcttcctc atccgccttg
       61 ttcttgctga tcaggcgaaa ctcattttcg atagaggact tcagcagccg gtcgaaagag
      121 tcttcaacgg ccttcgtcac ctccgcggca gccgggccgc ctttcacaaa tatcctgtcc
      181 aacgcatcca cggcggaagc cttgtcgata ttcaggtcca tgatcaggta accttccttc
      241 tcccctcgcc ggatggcgag aatgcgatgc gaaggacaat cggccagcgg ctcgttgaac
      301 tcgaaataat cccggtattt ctgggcttcc tcctcatcct tctttccggt gatgaccttg
      361 gcgctcaacg cagcggactc ggtgaagagc tgccgcacct tattccgcgc ctgttcattc
      421 tccgcgatcc actccgcaat gatatccctc gcgccctgca gagcgtcttt catatccttc
      481 acctgctcat tgataaactt ccctgcctct tccgccggat cacccgctcc ctgttcaaaa
      541 aggaatttcg ccagcggctc caggcccttt tcgacggccg tcgtcgccct cgtcttacgc
      601 ttgggcttat aaggcagata aatatcttcc agcaccgtcg cgtcataaca atggtcgatc
      661 cgctccttca acccgggagt cagcttgcca agcccttcga tggtcttgag caccgtctcc
      721 ttacgcttgt ccagttcgct gaaatatttt atctgctcca cgacatccgc aatctgcact
      781 tcgtccatat taccggtagc ttccttccgg taacgggcca taaagggaat ggtggccccc
      841 tccgcctgca aatcgaaaat gttattgact tgcttaaggc taaagtttaa tttttccgcg
      901 atcttctgta tataaactgg ttccatggtt gactaaaatg tgggcggcaa atgtaaggca
      961 aaatcctacc tttatcacat ggatatcaaa aacaatattc tcgagactat tgggcgcact
     1021 ccgttgatcc gtttgaacaa actcacccgg gagcttccct gcaccgtgct tgcgaaagta
     1081 gaatacttca accccggtaa ttccataaaa gaccgcatgg ctttgaaaat ggtggaggtc
     1141 gccgaacagg agggaaaact aagacccggc ggcaccatca tcgaatgcac cagcggcaac
     1201 accgggatgg gcctcgcact cgccgcagtg gtcaaaggct ataaatgcat cttcaccacc
     1261 accgataaac aatccaagga aaagatcgat atcctcaagg ccctgggcgc cgaggtcatc
     1321 gtctgcccca ccaatgtcga gccggaagac ccgcggtcgt actattccat cgcgcgacgc
     1381 ctcgcaaaag agatccccaa ctccttccac gcaaaccaat acgataacct ggccaaccgc
     1441 ctcgcccatt acgaaagcac cgggccggag atctgggagc aaaccgatgg gaagatcacc
     1501 catctcgtcg tcgccacagg cacgggcggg accgtaaccg gcacggccat gttcctgaaa
     1561 gaaaaaaatc ccgccatcca ggtatgggcc atggacccct tcggctccct gctgacaaaa
     1621 tatttccgca ccggtcaaat cgacatgggt gaggtccacc cctatatcag cgaaggcatc
     1681 ggcgaagact tcgttccgca gaattacgat atgcgtgtcg tagacgcctt tgaacaggtt
     1741 accgataagg acgcagccat catggcccgc cgcatcgccc gcgaagaagg cctcttctgc
     1801 ggctatagcg caggcaccgc cgtccagggc ctcatgcagc taaaggacct cctgaaaaaa
     1861 gaccacctcg tcgtcgtcat cctccacgat catggaagtc gttatgtcgg gaaagtatat
     1921 aacgaccagt ggatgatcga acggggcttc ctcgacgtaa agaccttcaa ggacatcatc
     1981 gccggccgcg gcgccaaacg gctgatcacc gccacaccgg aaaataccgt agcggaggcc
     2041 tttgagctca tgcaaaaata ccatatcgag aacctgcccg tgataaagga cggcgaaccc
     2101 atcggcgcca tcagcgaaag cggcctcttt tcccgcgtca tgaacaacgg ttccgatatc
     2161 aaagggaaaa ccatcgccga agtactggaa aaaccctacc ccatagtagc cttcgacact
     2221 ccgctggaaa ggcttagtac gctcattacc agggagaacg gagcggtgct cggaaaggat
     2281 gacagcggta attaccacat cgtcaccaag tatgatgtca tccacgcact gggagcctga
     2341 catgggtctg accccctcct gacatgggtc tgcccccata aaggaacgta gttttacgaa
     2401 cgacacaata gcaatcttcc tattgcatta tgcaatgatt acgaaatcgt tagtaaaatc
     2461 tgcgaccgtg aaataaagca aaacctctat tgccgtatca attttaacaa attatttttg
     2521 acctagaagt tgattgataa catcaatcac ccgatatcca aaaaaaccaa acaaacgccc
     2581 cggtggcaaa ccagaacctg gatccgttaa aaagaccgaa cccaatacct aagaaattcc
     2641 gtggtgaaac cgagttcacg aggttctttc cgacaattga aaagacattg tgaaccgggg
     2701 aaaaatccaa tatcagtcaa cttctacttt tttgatcgca actcgcaaaa gatataaggc
     2761 ccttaaagac taaaacgtat tcctcgaaat ccaacgtccg aacaagaacc tgaaaagcag
     2821 tcctatccga gaaacctact gctatcaaca gtacccctcc tgctaaatct gattagagca
     2881 acacacattt agcaggaggc cttcaccgac gggccgcacc ggcctcacct gcactctcct
     2941 ttttagttgt ttttcgtacc ttccggcatg ccgtccttta cagatcagtt aggcaggacc
     3001 attttcctgc cgcggatacc ccacaggatc atctccctgg taccctccca aaccgaatta
     3061 ctctacaccc tgggactgga cgaagccgtc gtcggcatca caaaattctg cgtccacccc
     3121 gaatcctggt ttcgcagcaa gccccgcatc gggggaacaa aggccatcga ccccgcccgg
     3181 gttgacgccc tgcaaccgga tcttatcatc gccaacaaag aagagaacga gaaaccccag
     3241 atcgaggccc tcgccacccg ctatcctgtc tggatcagcg acgtccatac actccaggac
     3301 gcccttgcca tgatccgcga cgtcggcgcc atcaccggga aggccaccga agcctccgca
     3361 ttggccacga cgatcgccac ccgcttccaa cagctcccca tccccgtcaa acctgttcct
     3421 gccgcttatc tcatctggag aaacccctgg atggccgccg gcggcgacac ttttatcaat
     3481 gatatgcttc gctattgcgg ctacaccaat gtcttttcag gccacgaccg ctatcctacc
     3541 ctcgaactct ccgccctcgc cggcccaaac tgcccgcata tcctcctttc ttccgaaccc
     3601 tatcccttcc gcgaacgcca catcaccgaa ataaaggaag tactgcccga cgccacgatc
     3661 cggctcgtcg acggtgaatt cttcagctgg tatggcagcc gcctgctcga agcccccgct
     3721 tactttagac agttgcagtg aaaatccgat atttgaagga tcatgcaatt cagcgatcag
     3781 cccacaggct ccgaatccaa caaacccagc cggaaaaaac ccattttccc ggtgaacgag
     3841 ggcctgcgca attatctcaa acaacacggc agggaagtca aactccccgt atcctaccgc
     3901 gatctcctcc tgcacatcac ctattccgtt cccctcaaag acaaacacca ccaggacacc
     3961 ctctgggaaa cggccctcta cgacatgcgc cactgggatt tcatccgcga aggcctcgtc
     4021 cagatgtatg ccatcctcaa gaccgaaggc gatctcagct ataccaaaca cctggacgtg
     4081 gcccgcatcg acttctgcag cttcgccaac tcccacccct ttcgcatccg tatcgtcaac
     4141 aagttcaacg acaactacga ccattactat atcaaacagg ccgatgcctc ccgcatctat
     4201 ggcctggaac tcgaacacct cctgtcgccc aaccggatca cgtttttcac ccaccacaac
     4261 accctcgttg aagaacatat ccccggcatc ccgggcgatc tctttatcca gaacttcctc
     4321 gaggacccgc agaccaataa gatccggctg gcaaaagagt tcgtaaaatt caacgaacgg
     4381 tgcttcgtcc ggctcctggg agacatgcga tcctataatt tcgtagtcga catcaccccc
     4441 gatatcgaag actaccaata caggatcagg gccatcgact tcgaccagca atcctacgaa
     4501 ggccggaaga acctctatat cccccagttc ttcaaagaga accgggcctt tgtcgatctt
     4561 tgtctcaacc acctgaacaa agagtccatc gaacaatacc agacggaaga acggacgatg
     4621 atggccttcc gggtcgcctc ctcccgcttc cggctaatgg aactgttaaa cataatggcc
     4681 tg
//
LOCUS       DATGFG010000631         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_867201, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000631 DATGFG010000000
VERSION     DATGFG010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867201"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1973)
                     /locus_tag="VKQ52_11830"
     CDS             complement(99..1973)
                     /locus_tag="VKQ52_11830"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLZ87929.1"
                     /translation="MRLPLFCSFFFFFFCSGWGLAQTSLSVPLNIREAINKGTRTVTG
                     QPGEHYWQNRADYSIQVRFDPRTRQVSGTETIVYTNNSPDTLRQLLFKLYPNLYKKGA
                     PRMQGVRPEDLTDGVAVSNLLIEGQKPATNFPASAAGTDVTIRIPATGPGRSVRVALD
                     FSYTLNKTSHIRTGQVDSGAFFIAYFFPRIAVYDDIDGWNRNPYLGHLEFYNDFCRFQ
                     VEVTVPGDYMVWGTGDLVNRRELLSADCNQRIDEAETKDGVVTIVDTGDLARGDVLRG
                     ADSNTWKFQAADVTDFVFAVSDHYIWKSSSLVVDGATGRRTRVDAVFNPAHRDFYEVV
                     DFARKTVEGMSYRFPAWPYPYPHETVFDGLDQMEYPMMVNDNPLSRREDVIELTDHEI
                     FHTMFPFYMGTNETKYAWMDEGWASIGEWLISPMIDSTIVDEYGVSSYASLAGREVDL
                     PIMTLSTQESGGTYFLNAYVKPAMGYLYAKDLLGDSLFRQGLHRYIRDWHGKHPMPLD
                     FFGSMNAGSGRNLDWFWKRWFYDDGYPDLGIKEVQHKGGAAAVVVVNTGGKPVPVDLT
                     IEFADGSKKNIHRSAAIWEKGNGETVIPVTSAKAISRVSLGSTYTPDSHPADNVWKAH
                     "
     gene            2092..>2477
                     /locus_tag="VKQ52_11835"
     CDS             2092..>2477
                     /locus_tag="VKQ52_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87930.1"
                     /translation="MKPLRLPFTLLCAWAAICLPARAQNSFPQLGKNSISEVIGAMTL
                     EEKASLVVGNGLVIPGFDLPGNNPAQLNDAQKKVPGASGVTRGIPRLGIPPIVVTDGP
                     AGVHVFNTGASRIYYATAWPVGTLLA"
BASE COUNT          568 a          833 c          578 g          498 t
ORIGIN      
        1 atctatcata gatcagcata ttatagttag caaaaacgca ccgccggccc ctcccaccgc
       61 cggtccctcc caccgccggc ccctcaacgc cggccgcctc aatgcgcctt ccacacatta
      121 tccgccggat gactgtccgg cgtataggtg ctgccgagac ttaccctgct gatcgccttc
      181 gcagacgtaa cagggatcac cgtttcgccg ttccctttct cccagatcgc tgccgatcgg
      241 tggatgttct ttttgctccc atcggcaaac tcgatcgtta gatccaccgg caccggcttg
      301 cccccggtat tgaccacgac gacggcagcc gcgccaccct tgtgctgtac ctcctttatc
      361 cccagatcgg gatacccgtc atcgtagaac cagcgcttcc agaaccaatc caggttccgg
      421 cccgacccgg cattcatact gccgaagaaa tccagcggca tcggatgctt cccatgccag
      481 tcccggatat accggtgcag cccctgccgg aacaggctgt cgcccagcag gtcttttgca
      541 tacaggtacc ccatcgccgg cttgacatag gcgttgagaa aataggttcc gccgctttcc
      601 tgcgtggaca gggtcatgat cggcagatcg acctccctgc ccgcaagcga cgcataggac
      661 gacaccccat attcgtcgac aatggtcgaa tcgatcatgg gcgagataag ccactccccg
      721 atgctcgccc aaccctcgtc catccacgca tatttcgttt cattcgtgcc catataaaaa
      781 gggaacatcg tatggaagat ctcatgatcg gtgagctcga tcacatcctc tctccggctt
      841 agcgggttgt cattgaccat catcgggtac tccatctggt cgagaccatc gaacaccgtt
      901 tcatgcggat aaggatacgg ccacgccggg aaccggtaac tcataccctc caccgtcttc
      961 cgggcaaaat cgaccacttc gtaaaaatcc cggtgcgccg ggttaaaaac ggcatccacc
     1021 cgcgtccgcc ggcccgtcgc cccatcaacg accaggctgg aagacttcca gatataatga
     1081 tcactcaccg caaacacaaa atccgtaaca tcggcggcct ggaatttcca ggtattcgaa
     1141 tccgcaccac gaaggacatc cccccgggcc agatcacccg tatcgacgat cgtaaccacc
     1201 ccatcctttg tctccgcctc gtcgatccgc tgattacagt cggccgataa caactcccgc
     1261 cggttgacaa gatcacccgt accccaaacc atataatcac cgggaaccgt cacctcgacc
     1321 tggaagcggc aaaaatcatt gtaaaattcc agatggccca ggtaagggtt ccggttccac
     1381 ccgtcgatat catcatatac cgctatccgg ggaaagaaat aggcgataaa aaaagccccg
     1441 ctgtccacct ggcccgtccg gatatgcgaa gtcttattga gcgtatagga aaagtccagg
     1501 gcaacccgaa ccgaccggcc cggccctgtc gccgggatcc gaatggtaac atccgttccc
     1561 gcagcggacg ccggaaaatt agtcgccggc ttctgcccct cgatcaacag gttggaaacc
     1621 gcaaccccat cggtcagatc ctccggccgc acaccctgca tccgcggcgc cccctttttg
     1681 tagagattag ggtataattt aaaaagcaac tgccgtagcg tatccgggct attattggta
     1741 tacacgatcg tctccgtccc actcacctgc ctggtccggg gatcgaatcg cacctggatg
     1801 gagtaatcgg cccggttctg ccagtaatgc tcccccggct gccccgtcac cgtccgggtc
     1861 cccttgttga tcgcctcccg gatattcaac ggcacagaca gggaggtctg ggccagcccc
     1921 cacccggaac aaaagaagaa gaagaagaag gaacaaaaga gcggtagtct cattggcata
     1981 agacggcgtt tttttatcaa tataaagcct aaatcccgga tctcggcctc ccctcaacca
     2041 cccttttttg ccggtaaaaa aaattattct acattgtgtc aaaataacat aatgaaacct
     2101 ctacgcttgc cattcaccct tctctgcgcc tgggctgcca tctgcctccc cgcccgggcc
     2161 cagaacagct tcccgcaact gggcaaaaac tccatctccg aagtgatcgg cgccatgacc
     2221 ctcgaagaaa aagccagtct cgtcgtaggc aatggtcttg ttatccccgg tttcgatctc
     2281 ccgggaaata accccgctca actgaacgat gcgcaaaaga aagtacccgg cgcctccggc
     2341 gtcacccggg gtatcccgcg tcttggcatc ccccccatcg tagtcacgga cggccccgcc
     2401 ggcgtacacg tgttcaacac cggagcgagc cgcatctatt atgccaccgc ctggcccgtc
     2461 gggacgctgc tggcaag
//
LOCUS       DATGFG010000632         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_232097, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000632 DATGFG010000000
VERSION     DATGFG010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232097"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1514
                     /locus_tag="VKQ52_11840"
     CDS             153..1514
                     /locus_tag="VKQ52_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ87931.1"
                     /translation="MRLRYKPALLALICGLLIVSSCKKSFFTDQNVNPNALPTVTPDL
                     LLPTVEAALGYTQGGDISRYSALLMQQMFGANSQSQQYYQYNFNPGNFDNLWPDYYTS
                     TLENDYTLIQIADAGGYNRYGGIARIIMAYGLQVGVDLWGDLPYSQAFKGNASEANLH
                     PSYDKAAGLYDTIVSLINVGTSLLQSPNTGSLTPGTQDVVFGGNAAAWIKFGHAIKAR
                     LALHRSKGSAQMAATALQEIAQAFASNKDNAQYTFGTQQTSANPWYQFYRDRPGDENF
                     PSSTLSVNMLADNDPRYVAFDLDSAVGGNAVSYYNQVNSPVEFITYDELLFASAEATL
                     RNGGSVATAQGFYQAAITANMKKLGVAQTDMDTYLAAHGTLPVTSDAAIAAIASEEYI
                     ALFLNPETWTLWRRTGVPSLTSVSGNAIPRRLLYPQSEINFNGSNVPQNVTLTSPKIF
                     WDN"
     gene            1708..3024
                     /gene="eat"
                     /locus_tag="VKQ52_11845"
     CDS             1708..3024
                     /gene="eat"
                     /locus_tag="VKQ52_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HLZ87932.1"
                     /translation="MSNQLNRSLGPLLLWGLGVGYVISGMYFGWNLGLAEGGTAGLAI
                     ATAGVILLYITFTFSYTELACAIPRAGGVFDYATRGLGRDWGFVAGMAQWIEFLFAPP
                     AIAAAIGAYFHLFLPEIPVTVIAIAAYFVFTALNIYGVKAAAGFELFVTVFAVVELLI
                     FSGVCFPHFSFKNLDRNAFPHGWTGVWAAVPFAVWFFLGLEGVANVAEEAKRPQRDIL
                     IGFGSALVTLIILCVLVFVSSIGVAGWEAIVYPTPGAADTSDSPLPMALSKITGTSGW
                     VYHLLITIGLMGLVASFHGLVLAAGRATLEFGRVRYLPVMIGRIHSRRGTPANALVVN
                     MVIGIVALLSGRTGDIIIISVFGALTLYVLGMVTVLVLRRKEPGLARPFRVPWYPVFP
                     IVALVIAVVCLVAMITLNIKLSLIYFSILLVSYLWFRLTAGGRRDR"
     gene            3048..>3463
                     /locus_tag="VKQ52_11850"
     CDS             3048..>3463
                     /locus_tag="VKQ52_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87933.1"
                     /translation="MQNNQSLHELLARLGEEQVEKVKLAIADIDGILRGKVIGFDKFK
                     SIASTGFGFCDVVFGWDAMDRAYDNGVFTGWHTGYPDAVAVVDVETMRRVPWEGGIPF
                     FLADFRASGSGVGSGGDAVGAMAVCPRSLLKRVIGQA"
BASE COUNT          605 a          777 c         1170 g          911 t
ORIGIN      
        1 tgtgtggacg aagtatgacg gtgtcgaccc cgagaccagc ctgggcgggc ctaataacca
       61 gcagggtttg gactatttca ataatcccaa taccaagagt tatggccttc gcctcaatgt
      121 cggcctttag ttcaactata aaaatgaata aaatgagact aagatataaa ccagcattac
      181 tggcattgat ctgcgggctg ttgatcgttt cttcgtgtaa aaagagcttt ttcaccgatc
      241 agaatgtcaa cccgaatgca ttgcctacgg tgacgccgga tcttttgttg ccgaccgtgg
      301 aggcggcgct tggctatacg cagggtggcg atatatcgcg ttactcggct ttgctgatgc
      361 agcagatgtt tggcgccaac agccagtcac agcagtatta ccagtataat ttcaatcccg
      421 ggaatttcga taatctctgg ccggattatt atacttcgac gctggagaac gattatacgc
      481 tgatccagat agccgatgcg ggtgggtata accggtatgg aggcatcgcg cggatcatca
      541 tggcctatgg gttacaggta ggtgtcgatc tttgggggga ccttccctat tcccaggctt
      601 tcaagggtaa tgcgtcggaa gccaacctgc accccagtta tgacaaggct gccgggctgt
      661 atgacactat cgtttcgctg atcaatgtcg gaacgtcgct gttgcagtcg ccgaatacgg
      721 gttcgctgac gcccggtacc caggatgttg tctttggcgg gaatgcggct gcctggatca
      781 agttcgggca tgcgataaag gcccggctgg ccctgcaccg gagcaagggt agtgcgcaga
      841 tggcggctac ggctctgcag gagatagcgc aggcctttgc ctccaataaa gacaatgcgc
      901 aatacacctt tggtacgcag cagacctcgg ccaatccctg gtaccagttt taccgtgacc
      961 ggccggggga tgagaatttt ccgagcagta cgctgtcggt gaatatgctg gcggacaatg
     1021 acccgcggta tgtcgctttc gaccttgaca gtgccgtggg tggcaatgcc gtcagctatt
     1081 acaaccaggt caattcgccg gtggagttca tcacttatga tgagttgttg tttgcaagcg
     1141 ccgaggccac gctgaggaat ggcgggagtg ttgccactgc gcagggcttt taccaggcgg
     1201 cgatcacggc caatatgaag aagctggggg ttgcgcagac cgatatggat acttatctgg
     1261 ccgcgcatgg gactcttccg gtgacatcgg atgcggctat tgcggcgatc gcgtcggagg
     1321 agtatatcgc cttgttcctg aacccggaga cctggaccct gtggcggcgt acgggcgtgc
     1381 cttcgctgac gagtgtttcg ggtaatgcca tcccgaggcg gctgttgtat ccgcagagcg
     1441 agatcaattt caatgggtcg aatgtgccgc agaatgtgac cctgacctca ccgaagatat
     1501 tttgggacaa ttgatgagag gtggagggat tgtttttgat ttttgggccc cggcgactgc
     1561 cggggccttt ttttggaggg ggcgccaccg gggatggcgg tttgggggag tttgggatgg
     1621 ttggaggatt tttgccggtg aggcgttttt tggtgtttgg gggaattttg cggtttgggc
     1681 gatttgccgc aattgttatc tttagggatg tccaatcaat tgaaccgtag ccttggtccg
     1741 cttttgttat gggggcttgg cgtggggtat gtcatttccg ggatgtattt cggctggaat
     1801 cttgggttgg cggagggggg tacggcggga ctggccattg cgacggccgg ggtcatcctg
     1861 ctttatatca cttttacgtt ctcctatact gagttagcct gtgccattcc gcgggccggg
     1921 ggggtattcg actatgccac ccgggggctt gggcgggatt ggggttttgt cgccgggatg
     1981 gcgcagtgga tcgagttcct ttttgccccg ccggcgatcg cggcggcgat cggggcgtat
     2041 tttcatcttt ttcttccgga gatcccggtg acggtgatcg cgatagcggc ttattttgtg
     2101 tttacggccc tgaacatcta tggcgtgaag gcggcggcgg ggttcgagct tttcgtgacg
     2161 gtatttgccg tggtggagct gctgatcttt tcgggggttt gtttcccgca tttttccttt
     2221 aagaacctgg accggaatgc tttcccgcat ggctggacgg gagtctgggc ggcggttcct
     2281 tttgccgtgt ggttctttct cgggctcgag ggagttgcca atgtggcgga ggaggcgaag
     2341 cggccgcagc gggatatact gatcgggttt gggtcggcgc ttgtgacgct gatcatcctg
     2401 tgtgtgctgg tgttcgtgtc gtcgatcggc gttgccggat gggaggctat cgtgtatccc
     2461 acaccgggag ccgccgatac ttcggattcg ccgttgccga tggctttgtc gaagataacg
     2521 ggtaccagcg ggtgggtata tcatttgctg atcacgatcg gcctgatggg attggtagct
     2581 tcgttccatg gattggtgct ggcggcgggc agggcgaccc tggagtttgg ccgggtgcgt
     2641 tatcttccgg tgatgatcgg gcggatccac agccgcaggg ggacgccggc gaatgccctt
     2701 gtggtcaata tggtgatcgg gatcgttgcc ttgctgtccg ggcggacggg ggatatcatt
     2761 atcatttcgg tgtttggggc gcttaccctg tatgtgctgg ggatggtgac cgtgcttgtg
     2821 ttgcggagga aggagccggg gctggcccgg cctttccggg tgccgtggta cccggtcttc
     2881 ccgatagtgg cgctggtgat cgcggtggtg tgcctggtgg cgatgatcac gcttaatatt
     2941 aaattatccc tcatctattt ctccattttg ctggtgagtt atctttggtt ccgtttgacg
     3001 gccggaggcc ggcgtgaccg ataggtttga cattaaaaca aaggctgatg cagaacaatc
     3061 aatcgcttca tgagctgctt gcccggctgg gtgaggagca ggtcgaaaag gtaaagctgg
     3121 ccattgcgga tatcgacggt attttgcggg gcaaggtgat cggttttgac aaattcaagt
     3181 cgatcgcctc gactggtttt ggattttgtg atgtggtttt tggctgggat gcgatggaca
     3241 gggcgtatga caacggggtt tttacgggct ggcatacggg ttatcccgat gcggtggcgg
     3301 tggtggatgt ggagacgatg cggcgggtgc cgtgggaagg ggggatacct ttctttttgg
     3361 cggattttcg tgcgagcggt agcggggttg gctcgggggg ggatgcggtg ggggcgatgg
     3421 cggtttgtcc gcggagtctg ctgaagcggg tgatcggtca ggc
//
LOCUS       DATGFG010000633         4813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1464407, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000633 DATGFG010000000
VERSION     DATGFG010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464407"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="VKQ52_11855"
     CDS             <1..1843
                     /locus_tag="VKQ52_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLZ87934.1"
                     /translation="VQNIFALEQVLSGPGRNFIRAANGQEALKTVLNKEIDLVILDVQ
                     MPDMDGFEVAQILKSHHRTKDIPIIFASAEKKEHRFMMKGFEEGAVDYLYKPLDPAVT
                     EAKVSVLLQLHLQKKELVRKNIILEKHALLINNSADLICIINARTLKFEEINHAVGPM
                     LGYTVEEARNTSILFYLDREDRPAVNNLCKSDQEKFSFEVRLYAKDRSIRWLNWNVVN
                     KDGLWFANARDITGIKEAEEIKNYLSIVVKQSSDAIYLHDHEGKIISWNEGAEKMYGF
                     SEAEAVNMKIWNIVPEQLLAGMQTVISDLISGKKIQVQEMQRITKYGKMIDVLFSASV
                     ITDFSGGLKSVAITEREITQQKKADQEIRQLNADLKVNITQLEATNKELESFSYSVSH
                     DLRAPLRRINGYAGIILEDHRTQLDEELRRLFGNIKNNADRMSILIDNLLEFSKLGRR
                     RVAKVPVDLTELVQRVLKDLDEGERDKATIQIGYLPPAEGDPALLYQVLVNLVSNAIK
                     YSGKKAAPEIGIGSCENEEEYVYFVRDNGIGFNMTYAHKLFGVFQRLHNDDGFEGTGV
                     GLAIVQRIVVKHGGRVWAEGQPGEGATFYFSLPKPVTARNTIEYHEP"
     gene            1833..2957
                     /locus_tag="VKQ52_11860"
     CDS             1833..2957
                     /locus_tag="VKQ52_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ87935.1"
                     /translation="MNPEFNYPVRILILEHEPDDIALLQYELKKGGLQYSSVVVQDRA
                     NYEQTLRDFKPDLVLSDFVLPEFDGAAAFYLKKEIAPDIPFIIVSGTIGEENAVQLIK
                     AGVTDYVLKDKLFTVVTKIGRALKESQEHREKKEQEQRLENYARELERSNRELEEFAY
                     IASHDLQEPLRMVGSFLQLLQRKYQDKLDEEANEYIHYAVDGAARMKRLIGDLLNYSR
                     TNREWAIEQVDISATIREALRNLTTSINDSGAMVNFQDMPVLEADPVQMLQLFQNLIA
                     NAIKFRKEESPVIKIGAVKSDGHWLFSVEDNGIGIERQYAEKIFVIFKKLHNNIKFEG
                     TGIGLAVAKKIVERHGGKIWFESVPGTGTTFYFTLYPVNK"
     gene            2954..3382
                     /locus_tag="VKQ52_11865"
     CDS             2954..3382
                     /locus_tag="VKQ52_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ87936.1"
                     /translation="MKPITILLVEDNPGDVLLAQVAFEKSKFPVNVQVAMDGDEALAY
                     LYKKGRFGESPRPDLILLDLNLPAKSGAEVLADLKSDEELKAIPVIILTTSQAERDIV
                     LCYKLHANCFVNKPFGFESFDKVVTCIEDFWFDVVRLPGN"
     gene            3407..3994
                     /locus_tag="VKQ52_11870"
     CDS             3407..3994
                     /locus_tag="VKQ52_11870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ87937.1"
                     /translation="MTGTTAQRPPSPYTLIDKATRESGFTMASDVDVCAFLKTLAASK
                     PGGKFLELGTGTGLSTSWILDGMNASASLISVDNDPAVLQIAKQFLGDDHRLHLVESD
                     GAAWIEQNRNLKFDFIFADTWHGKYLMLEEVLDMLDPAALYIVDDMLPQENWPEGHQE
                     KAIGLVKKLESRTDLLISKLPWSTGVIVAVKCPGN"
     gene            3998..4708
                     /locus_tag="VKQ52_11875"
     CDS             3998..4708
                     /locus_tag="VKQ52_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HLZ87938.1"
                     /translation="MESIFENPESHYKVTFKRDGQSFLSMLGVIVKPFDRSLVSLLTR
                     EEIKIAEKYTGSRYSEFVHSRYITKRAVQQLMGDVPMDKIQLLKGVFEQPILEIAGSG
                     AGYDASVAHKDDIAASLIFPRYHPAGIDIDVTVPRNSNTLNRLITEHEQQVCRGDSEK
                     KMVIYTAKEALSKIIRCGLTVPVHFLEVNDLQKEEIFYCGVFTNFCQYRFMSFAGDEY
                     IITMVFPRNSNCTIEKMH"
BASE COUNT         1335 a         1167 c         1246 g         1065 t
ORIGIN      
        1 ggtacagaac atctttgcgc ttgaacaggt attgtccggc ccgggcagga attttataag
       61 ggccgccaac ggacaggaag cattgaagac cgttttgaat aaggagatcg acctggtcat
      121 cctggatgtc cagatgcccg atatggacgg ctttgaagtg gcccagatcc tgaaatccca
      181 ccatagaact aaggatattc ccatcatctt tgcatccgcg gaaaagaagg aacaccggtt
      241 catgatgaag ggattcgaag aaggcgccgt cgattatctc tacaaaccgc tggaccccgc
      301 cgtaacggaa gccaaggtct cggtccttct tcaactccat cttcaaaaaa aggaattggt
      361 caggaagaac atcatattgg aaaaacacgc cctcttaatc aataattccg cagacctgat
      421 ctgcatcatc aatgcccgga cattgaagtt cgaggagatc aaccatgcgg taggtcccat
      481 gctcggctat acggtggaag aggcgaggaa cacgtccatc ctgttctatc ttgaccggga
      541 agaccgtccc gccgttaata acctgtgcaa atccgatcaa gaaaaattct cattcgaggt
      601 gcggttgtat gccaaagaca gatcgatccg ctggctcaac tggaatgtcg tgaacaaaga
      661 cggcctgtgg tttgccaatg cgagggatat caccgggata aaagaagcgg aggaaataaa
      721 gaattacctc tcgatcgttg taaagcagtc cagcgatgcc atctatcttc atgatcatga
      781 agggaagatc atctcctgga acgaaggggc cgaaaaaatg tatggtttct cggaagcgga
      841 ggccgtcaat atgaagatct ggaacatcgt tccggaacag ttgctggccg ggatgcaaac
      901 cgtaataagc gatctcatca gcgggaagaa gatccaggtt caggaaatgc aacgcattac
      961 aaaatacggc aaaatgatcg acgtcctctt ttccgcctcc gtcatcacgg acttcagcgg
     1021 cggcctgaag tcggtcgcca tcaccgaaag ggaaatcacc caacagaaga aggccgacca
     1081 ggagatcagg caattgaatg ccgatctcaa agtgaacatc acccagttgg aagccacaaa
     1141 taaagaactg gagtcattct cttattccgt gtcccacgac cttcgggccc ccctcaggcg
     1201 tatcaacggg tatgccggga tcatactgga agatcatcgc acgcaattgg atgaggaatt
     1261 gcgccgtttg tttgggaaca taaagaacaa tgcggacagg atgtcaatcc tgatagataa
     1321 cctgcttgaa ttttcaaaat tagggcggcg cagggtggcg aaggtcccgg tggatctcac
     1381 ggaactcgta caaagggtcc tgaaagacct ggatgaaggc gaaagggaca aggcgaccat
     1441 acagatcggc tatctgcccc cggcagaggg agacccggca ttgctctacc aggtgctcgt
     1501 taacctcgtc tccaatgcga tcaaatactc cggtaagaag gcagcgccgg agattggcat
     1561 cggttcctgt gaaaatgagg aggaatatgt ctattttgtg agggacaacg gaataggatt
     1621 caatatgacc tatgcgcata aattgttcgg cgtgttccag cgactgcaca acgacgatgg
     1681 gttcgaaggc acgggagtag gcctggccat cgtccagcgg atcgtggtaa aacacggcgg
     1741 aagggtatgg gcagaagggc agccgggtga aggggcgaca ttttacttct ccctgccaaa
     1801 accggtaacc gcaagaaata ccatcgaata ccatgaaccc tgaattcaac tatcccgtca
     1861 gaatactgat cctggaacac gagccggacg acatcgcctt gctgcaatat gaactgaaaa
     1921 agggcggttt gcagtattca tcggtagtcg tgcaggatcg cgcgaattat gaacaaaccc
     1981 tccgggattt caagccggac ctggtgctct ccgattttgt ccttcctgaa ttcgatggcg
     2041 ccgccgcctt ttatctgaag aaagagatcg ctcccgacat cccctttatt attgtctccg
     2101 ggacgatcgg tgaggaaaat gcggtgcagc tgatcaaggc cggggtcacc gactacgtgc
     2161 tgaaagacaa attatttacc gtagtaacca agatcggccg ggcattaaag gagagccagg
     2221 aacacaggga gaaaaaggag caggagcaac ggctggaaaa ctatgcccgc gagctcgaac
     2281 ggtccaacag ggagttggaa gagttcgcct atatcgcttc gcacgacctg caggaaccgc
     2341 ttcgcatggt agggagcttc ctgcaactgc tgcagcggaa ataccaggat aagctggacg
     2401 aagaggccaa cgaatacatc cactatgctg tcgacggcgc cgcccgtatg aaacgattga
     2461 tcggcgacct gctgaattat tcccgcacga accgggaatg ggcgatcgaa caggtggata
     2521 tctcggctac catccgcgag gcgctgagaa acctgaccac cagtataaac gacagcggtg
     2581 cgatggtcaa tttccaggat atgcccgtac tggaggcaga ccccgtgcaa atgctccagt
     2641 tgttccaaaa ccttattgcc aatgccatca aattcaggaa ggaagagtcg cccgttataa
     2701 aaataggcgc cgttaaaagc gacggccact ggctgttctc cgtggaagat aatggaatag
     2761 gtatcgagcg gcaatacgcg gaaaagatct tcgtcatctt caaaaagttg cataacaata
     2821 taaaattcga gggtacaggt atcggacttg ccgttgcaaa aaagatcgtc gaaagacacg
     2881 gaggaaagat ctggttcgaa tcggtaccgg gaacaggaac gaccttttat ttcactttat
     2941 accccgtaaa caaatgaagc ctattactat ccttctcgtg gaagataacc ccggagatgt
     3001 cctgctcgca caggtagcct tcgaaaagag taagtttccc gtcaacgtac aggtggcgat
     3061 ggacggtgac gaagcattgg cctacctgta taaaaaagga aggttcggtg agtccccccg
     3121 gcccgatctg atcctgctgg acctgaatct gccggcaaaa agcggtgccg aagtactggc
     3181 cgaccttaaa agcgacgagg aattgaaagc cataccggtg atcatcctta ccacctcgca
     3241 ggccgaacgg gatatcgtac tatgttataa gctgcatgcc aattgcttcg tgaataagcc
     3301 gtttggattc gagtcctttg acaaggtggt gacctgcatc gaggatttct ggttcgacgt
     3361 cgtcaggctg cccggtaact aaagataaac ccattaagta tatactatga caggaacgac
     3421 agctcagcgc cccccctcgc cctacacgct tatagataaa gccaccaggg aatccggctt
     3481 tacaatggcc tccgatgttg acgtgtgcgc ttttttaaaa acactggccg cttcaaaacc
     3541 gggcggcaaa ttcctggaat tgggaaccgg caccggtctg tcgacgtcct ggatcctcga
     3601 tggaatgaat gcttccgctt cactgatctc cgttgacaac gaccccgcgg tactgcagat
     3661 cgcaaagcag ttcctgggcg atgaccacag gttacacctg gtagagtcgg atggcgccgc
     3721 ctggattgaa caaaacagga atttgaaatt tgattttata tttgccgata cctggcacgg
     3781 caagtatttg atgctggaag aagtgctgga tatgttggac cctgcggccc tgtacatcgt
     3841 agacgatatg ttaccccagg aaaattggcc tgagggacac caggaaaaag ccatcgggct
     3901 ggtcaaaaaa ctcgaaagca ggaccgattt gctgatttcc aaacttccct ggtctaccgg
     3961 agtgatcgtt gccgtaaaat gtccgggtaa ttaaccgatg gaatccattt tcgaaaaccc
     4021 ggaaagccat tataaagtta cctttaaaag ggacgggcaa tcctttttgt cgatgctcgg
     4081 cgttatcgtc aaaccatttg accgatccct ggtttcactg ctaacccggg aagaaataaa
     4141 gatagcggag aaatatacgg gttcaagata cagtgagttt gtccactcca ggtatattac
     4201 caaaagggct gttcaacaac tcatgggtga tgtaccaatg gataagatcc agctgctgaa
     4261 aggcgttttt gaacagccta tcctggaaat tgccggcagc ggcgccggct atgatgcgtc
     4321 tgtagcccat aaggatgata ttgcggcttc tctcatattc ccccggtatc acccggccgg
     4381 tatcgatatc gatgtaaccg tcccccggaa tagcaatacc ctgaaccggc tgatcaccga
     4441 acatgaacaa caggtctgca ggggtgatag cgaaaagaag atggtaattt atacggcaaa
     4501 ggaggcgctt tccaagatta tcagatgcgg gctgacggtc ccggttcact tcctggaggt
     4561 aaacgatctt caaaaggaag agattttcta ttgcggtgtc ttcaccaatt tctgccagta
     4621 tcggtttatg tcctttgccg gagatgagta tattattacc atggtttttc caaggaatag
     4681 caactgcacc atcgaaaaaa tgcactaacc gggccgttat ttctgcagtt cataaatgga
     4741 tgagatcttg tccttataaa cgggaattac gatgtcattc atggctactt ccgcggtgaa
     4801 ttcatttttt ccc
//
LOCUS       DATGFG010000634         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1443913, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000634 DATGFG010000000
VERSION     DATGFG010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443913"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VKQ52_11880"
     CDS             <1..372
                     /locus_tag="VKQ52_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87939.1"
                     /translation="PDNGRYHFGPTDTALMAKLTVVSRDSMRYTAYPVIYVKNNETHY
                     LVDTVFAQDLALRFNKITDDQRIELGMKESGDMVPFIAMKVLEFPQINILWIGTIIMI
                     SGFVMSLLWRRRQAKVKMAVR"
     gene            488..1087
                     /locus_tag="VKQ52_11885"
     CDS             488..1087
                     /locus_tag="VKQ52_11885"
                     /inference="COORDINATES: protein motif:HMM:NF025492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HLZ87940.1"
                     /translation="MTTMSASAQTADTTLPLNIPRPEYGPYDTIIVPAKIYGGELLPA
                     NTIEYTWVRAPMTPAMRRRFEEWTRLRNAVYVTYPYARKAGMIMNEMNAKMALLSTEK
                     QRKEYIQSREKDLKKEFTEPLEQLSIYQGKILMKLINRQTGNNCYDIIKEYKGGFTAR
                     FWQTVAFFFSSNLKQPYDPKGDDRAIEVIVQEVERMYHS"
     gene            complement(1185..1784)
                     /locus_tag="VKQ52_11890"
     CDS             complement(1185..1784)
                     /locus_tag="VKQ52_11890"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLZ87941.1"
                     /translation="MKRLLLLLGATLGIAAGAAAQSTAASSVRSTAASSPQPPAASTL
                     RSTAASSPQPAVASSVRSTAASPARSTLPSTDRSADIAAIKQLNADWIHNYMVKDTAT
                     FDRIFADDFILINPSGKKFTKKDVIANIGQPIVSAKVDTVEVQLHGNIGLVIAKCSFV
                     TRAEGKESAGQTDYLDVYEKRKGRWWAIVAHVTYLGSPQ"
     gene            complement(1781..2272)
                     /gene="rfaE2"
                     /locus_tag="VKQ52_11895"
     CDS             complement(1781..2272)
                     /gene="rfaE2"
                     /locus_tag="VKQ52_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HLZ87942.1"
                     /translation="MKVPQIIQQKIYDLPGMRHQLRRWRLLSKTIAFTNGCFDILHQG
                     HIASLSDAAREADILIVGLNSDASTRRLKGEQRPINNQEARATVMASLLMVDAVIIFD
                     EDTPLDLIKTVMPDVLVKGGDYTLEQIVGAKEVMEAGGRVVINPIVPGFSTTGIIDKI
                     CRL"
     gene            complement(2235..>2621)
                     /locus_tag="VKQ52_11900"
     CDS             complement(2235..>2621)
                     /locus_tag="VKQ52_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87943.1"
                     /translation="KPLFFVYTVGIWCLYLLSTWFGFFAISATSHLTIIDALSVLAMG
                     SVGMIVSPGGIGAYALLVQETVSFYHVPAIPFGLALGWLLWFGQFLSFILFGTVSFIL
                     LPRINKKPHYQIIAHEGPADHPTKNL"
BASE COUNT          550 a          592 c          897 g          582 t
ORIGIN      
        1 cccgataatg gcaggtatca tttcgggcct accgatacgg ccctgatggc gaagcttacg
       61 gtggtgagca gggacagtat gcggtatacg gcctatcccg tgatctatgt gaagaataac
      121 gagacgcatt atcttgtcga cacggtattt gcgcaggacc tggccctgcg gttcaacaag
      181 atcaccgatg atcagcgcat cgagctgggc atgaaggaat ccggcgatat ggtgcctttt
      241 atagccatga aggtgttgga atttccgcaa attaatatcc tgtggattgg gacgatcatt
      301 atgatcagtg gcttcgtaat gagcctgttg tggcgccgcc gccaggcaaa ggtcaaaatg
      361 gcggtccgct aagggttacc cggagggggt atggggtatt aattagtgag gttttttagt
      421 aattttaaga ccaatgcgct tccgtgtaca caaatacctg ttgtcattat ctgttattct
      481 attggtaatg accacaatga gcgcatccgc ccaaaccgcg gacaccacgc ttcccctcaa
      541 tattccacgg ccggagtatg gtccgtatga tacgatcatc gtgccggcca agatctatgg
      601 cggagaattg cttccggcca ataccatcga atatacctgg gttcgcgctc cgatgacacc
      661 ggccatgcgg cgccgtttcg aggagtggac gaggctgcgg aatgcggtgt atgtgacgta
      721 tccctatgcc cgcaaggcgg ggatgatcat gaacgagatg aatgcgaaga tggccttgct
      781 cagcacggaa aagcagcgaa aggagtatat acaatcccgt gagaaggacc tgaagaagga
      841 attcaccgag ccgctggagc agttgtcgat ctaccagggg aagatactga tgaagctgat
      901 caaccggcag accggcaata attgttacga tatcatcaag gaatacaagg gagggtttac
      961 ggcgcgtttc tggcagaccg tggccttctt tttcagcagt aatctgaagc agccgtatga
     1021 tcccaagggg gatgaccgcg ccatcgaggt gatcgtgcag gaggtggagc ggatgtacca
     1081 cagttgatgt gggcggcggg cggcggtcag tgcggctcgg cggtttgggg tggcgtggct
     1141 cgccggaggg acggtggtca gtgcgctcgc cggaggggcg gtggtcattg cggcgacccc
     1201 agataggtta catgcgcgac gatggcccac cagcggcctt ttcttttttc atatacatcg
     1261 aggtagtcgg tctggccggc ggattctttt ccttcggcgc gggtgacgaa gctgcatttc
     1321 gctatgacga ggccgatatt gccatggagc tggacctcga cggtgtcgac ctttgccgag
     1381 acgatgggtt ggccgatgtt ggctattacg tcctttttcg tgaatttttt gccggagggg
     1441 ttgataagga tgaagtcgtc ggcgaagatg cggtcgaagg tggcggtgtc ttttaccatg
     1501 tagttgtgga tccagtcggc gttgagttgt ttgatggccg cgatgtctgc ggaccggtca
     1561 gtggagggga gggtggatcg ggcgggggat gcggccgttg accggaccga ggatgcgact
     1621 gccggttgag gtgaggatgc ggccgttgat cggagggtgg aggcggctgg cggttgaggc
     1681 gaggatgcgg ccgttgaccg gactgaggat gcggccgttg attgggcggc ggcgccggcg
     1741 gcgatgccga gggtggcgcc gagaaggagc aggagtcttt tcataaacga catattttat
     1801 cgatgatccc tgtcgtggag aagccgggaa cgatggggtt gatcacgacg cggccgccgg
     1861 cctccattac ttcttttgcg cctacgatct gttcgagggt atagtcgccg ccttttacga
     1921 ggacgtcggg catgacggtc tttatgagat cgagcggggt atcctcgtcg aagatgataa
     1981 cggcgtccac catgaggagg gaggccatga cggtggcgcg ggcctcctgg ttattgatag
     2041 ggcgttgctc gcctttcagg cgtcgggtgc tggcgtcgga gttgaggccg acgatgagga
     2101 tatcggcttc ccgtgcggcg tccgagaggg aggcgatgtg tccctggtgg aggatgtcga
     2161 agcagccatt cgtgaacgcg atggtcttac tcaggagtcg ccagcggcgc aactggtgtc
     2221 gcatgccggg aaggtcataa attttttgtt ggatgatctg cgggaccttc atgagcgatg
     2281 atttgataat gtggtttttt gttgatgcgc ggcagcagga tgaagctgac cgtgccgaac
     2341 aggataaagc taagaaattg tccaaaccaa agcaaccagc ccagggcgag gccgaagggg
     2401 atggccggaa catggtagaa ggagacggtt tcctggacga gcagggcgta ggcgccgatg
     2461 ccgccggggg aaacgatcat gccgacactg cccatcgcga ggacgctgag cgcgtcgatg
     2521 atggtgaggt ggctggtggc gctgatggcg aagaatccga accaggtgct tagcaggtag
     2581 aggcaccaga tgccgacggt atagacaaag aagaggggtt t
//
LOCUS       DATGFG010000635         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1032504, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000635 DATGFG010000000
VERSION     DATGFG010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032504"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VKQ52_11905"
     CDS             <1..1418
                     /locus_tag="VKQ52_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ87944.1"
                     /translation="DRDKLYVSGYYGKDNLSSADIFGLSWGNATATVRWNHTFGPRLF
                     SNTSLIYSNYNYQIRFANNGNDFTVNSVLRDFNVKEEFQYYLNPRNTIRFGVNSIDHR
                     LSPGVVDGGNGKSGVNDTSFQVRYAWENAVYVSNDWKATDRLNISYGFRGEFFSPFGR
                     GDYYTVDPAGRITDTLHNTGGGIIKTYFNPEFRVAFSYQLNPGSSVKASYARNTQNIH
                     LLSPGSIGQPTDRYVLSNNMIKPETSDQVSLGYYQNLQHDRYELTVETYYKALGNQID
                     YRDGADLHTNDHIETELLYGKGRAYGIEWLLRKKTGSLTGWIGYTLSRSEKKIDGINN
                     DQWYNAFQDRTHDISVVAMYKLNAKWNLSANWVYNTGNAISLPSGKYKSDGRTVYYYS
                     ERNGYRMPAYHRLDLGATVQLKQRPRFSSELAFGVYNAYARNNAYLITFRDNKNNPDQ
                     TEAVQTTLFSIVPFISYNFKF"
     gene            1424..2221
                     /locus_tag="VKQ52_11910"
     CDS             1424..2221
                     /locus_tag="VKQ52_11910"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HLZ87945.1"
                     /translation="MNHTFSTTTAFFIIILFTACQKVINLNLRTAPAKYVIEGNVTNL
                     PGPYQVTISQTGAVESKYSFNGVSQANVSIRDNAGNSETLREVQPGVYQTATLRGVEG
                     RRYDLTIRIGENSFTSTSTMPQQVNLDSLYTEQIYNFSKMVTAVVPLFTDPVGKGNCY
                     RFNQYINGNLDRTLYGENDDFTDGKRATWALLRPDPDSTLHPGDRVDVEMQCIDRPVY
                     DYWFSVDQASLGDDGGSQPGNPVTNIQGGAMGYFSAHTAQTKTILIK"
BASE COUNT          623 a          644 c          642 g          525 t
ORIGIN      
        1 gtgaccggga caagctctat gtatccggct attacggaaa agacaatttg tcgagtgcgg
       61 acatcttcgg gcttagttgg gggaatgcaa ccgcaacggt tcgctggaac cacacgttcg
      121 gtccccggct gttctccaac acctcgctga tctatagcaa ttacaattac cagatacgct
      181 ttgccaataa tggaaatgat tttaccgtga actccgtact gcgggatttc aatgtcaagg
      241 aagaattcca atattatctc aatcccagga ataccatccg gtttggcgtc aactccatcg
      301 atcatcggct gagccccgga gtggtagatg gcggcaacgg caagagcggg gtcaacgaca
      361 ccagcttcca ggtcaggtat gcctgggaga atgcggtata cgtcagcaac gactggaagg
      421 ctacggacag gctcaatatt tcctacgggt tcagaggcga gtttttcagc ccctttggca
      481 ggggtgatta ctataccgtg gatcctgccg gtcggatcac cgacacttta cacaataccg
      541 gcggtgggat catcaagacc tatttcaacc ccgagttcag agtggcgttc agctatcagc
      601 tgaacccggg cagctccgta aaagcatcct atgcccggaa cacccagaat atacacctgt
      661 tgtctcccgg cagcatcggg cagccgacgg atcgctatgt tctcagcaat aatatgatca
      721 agccggagac ttcggaccag gtatccctgg gatattacca aaacctgcaa catgacaggt
      781 atgagctgac cgtagaaacg tattataagg cgcttggcaa ccagatcgac taccgggatg
      841 gggccgatct gcataccaat gatcatatcg agaccgagct cctgtatggc aaggggcggg
      901 cgtatgggat agaatggctc ctccggaaga aaacgggcag cctgacgggc tggatcgggt
      961 atacgctctc caggtctgaa aaaaagatcg acgggatcaa caatgatcaa tggtacaatg
     1021 ccttccagga caggacacat gatatttccg tcgtggccat gtacaagctg aacgccaaat
     1081 ggaatttgtc cgccaactgg gtatataaca cgggcaatgc gatcagtctc cccagcggca
     1141 agtataagtc ggacggccgc acggtctatt actattcgga aaggaatggt taccggatgc
     1201 ccgcttatca ccggctggac ctgggtgcga cggtgcagtt gaagcagcgc ccccgcttct
     1261 cctcggagct tgcattcggc gtatataatg cctacgcaag gaataatgcc tacctgatca
     1321 ccttccggga caataagaat aatcccgacc aaacggaggc ggtacagacc accctgttca
     1381 gcatcgtgcc attcatctcc tataatttca aattttaacc gccatgaacc atacatttag
     1441 tactaccact gcttttttca ttattatcct gtttacggct tgtcagaagg tgatcaatct
     1501 gaacctgcga actgctcccg caaaatatgt gatcgaaggt aatgtcacca atctccccgg
     1561 tccctaccag gtaacgatca gccagacggg agcggtagag agtaagtata gctttaatgg
     1621 cgtcagccag gcgaacgtct ctatccggga taatgcgggc aatagcgaaa cgctccggga
     1681 ggtgcagccg ggggtctatc agacggccac gctgcggggt gtcgaggggc ggcgttatga
     1741 tctcaccatc cgaatcgggg agaatagctt tacctctacg tccacaatgc ctcagcaggt
     1801 aaacctggat agtctctaca ccgagcagat ctataacttc tcaaagatgg tgaccgctgt
     1861 agtgcctttg ttcaccgacc cggtgggcaa aggaaattgc taccggttca accaatatat
     1921 caatggcaac ctggacagga ccttgtacgg ggagaacgac gacttcacgg atgggaagcg
     1981 agccacctgg gccctgctcc ggccggaccc cgatagcacc ctgcatcccg gtgaccgggt
     2041 ggatgtggaa atgcagtgta tcgatcgccc agtgtacgac tattggttca gcgtcgacca
     2101 ggcttccctg ggagacgatg gcggcagcca gccgggtaat ccggtaacga atatccaggg
     2161 aggggcaatg gggtatttca gcgcgcatac ggctcaaaca aagaccatcc tgattaaata
     2221 gggggattgg gctgtagtat ataggagtcc gtgggttgaa ggtattcaaa aaattgtcta
     2281 ttttgagggt ccaacctaag cctatgccta aatactccgt cctgaacctg tccgccgccg
     2341 cattgctcct ggtcaccgcg ctcctgcctg cgtgtagcaa aacgggcagc aaagccaacg
     2401 gccaacccaa tcccaaagac acgatccaga tcac
//
LOCUS       DATGFG010000636         4905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_588284, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000636 DATGFG010000000
VERSION     DATGFG010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588284"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="VKQ52_11915"
     CDS             complement(<1..1790)
                     /locus_tag="VKQ52_11915"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ87946.1"
                     /translation="MSKPFILAPLFVAFFFLSAHSQVRIRGSVRGIVADTAGKLPMSD
                     ATVSVTPERDTSDAQFAITDKRGGFLFRGLATGGYHLVITFEGYRHVRKNFVIDGTTR
                     DIDLGAVLMERAPDILAEVVIQRPPMTIKKDTIEYNAESFATKPNAVAEDQLKKLPGV
                     QVDASGNITAQGEKVQRLLVNGKRFFGNDPKLATRNIPPEIIEKYQIFDDLDDQSKFS
                     GFDDGNRIKTINIITRKDKRQGYFGRGIAGAGTDQTYNESAVLHHFNNDRQISALEEG
                     NDINKQNFTVQDVLGSSGSRRGSGGGPGAAANPSAAGITTVWAGGANYRDSWGKYDAY
                     GSYFYNFTHVSSNTSSLTQKFFTTAEDTSNSITQTSPAISRSTNQRLNFNIENKNDSG
                     NSFVFRPNITWQHTSPNSSQTTTTVDQDGRPVSSSIGNSYSSNDGFNINGSNLTLRHK
                     FRKPYRTISLDVTGTVNVNDGSGYYHSANTFYKLDSLQDLRQFYTDSLHSVNIAPTLS
                     YTEPISKHSIIEVNYNHTYNRSRTINNTYDYSDAAKGYTSFDSLFSNSYRFESNSDRV
                     SLNYRLQHAKLNVNIGSGVQFMNFDSKNTT"
     gene            1950..2582
                     /locus_tag="VKQ52_11920"
     CDS             1950..2582
                     /locus_tag="VKQ52_11920"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HLZ87947.1"
                     /translation="MKALNILALFLSIGTAGHAQFEGVLTYDCTIRNKTLTTVYESRT
                     KVLLEAKIYPMKAGIADIREAREQDPILFDFEAKKATRIGAKHHETISSDLAPVTNDH
                     NDKAKDGDITITLVGPEKIDAYNCQHFVVKIKNTTTELWITKDLGVSEICMLSQFDYY
                     PAGSILYDKLKAAGGDGIVVRSKTGEVNVNLTNAQVKTVPPSYFEIPGKP"
     gene            complement(2788..3171)
                     /locus_tag="VKQ52_11925"
     CDS             complement(2788..3171)
                     /locus_tag="VKQ52_11925"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HLZ87948.1"
                     /translation="MQEKQLFEYAVIRVVPRVDREEFLNVGVILYCHGSRALGLKYRV
                     DEERLRTLAPTLDIAEVEAHLKGFEEIVNGGEAGGPIGKLDGAGRFRWLTATRSTIIQ
                     TSRVHPGFCGDFAGELQRLYETMVL"
     gene            complement(3184..4029)
                     /locus_tag="VKQ52_11930"
     CDS             complement(3184..4029)
                     /locus_tag="VKQ52_11930"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HLZ87949.1"
                     /translation="MSISIPELRQVAVTRYVTPLREGGSLPAIAEADDGFLYVLKFRG
                     AGQGVKSLIAELIGGEIARALGLRIPEIVFAQLDAAFGRSEADEEIQDLLKASVGLNL
                     GLHYLQGSITFDPVVNRVDETMASQIVWLDCLLTNVDRTARNTNMLVWNKELWLIDHG
                     ASLYFHHSWDESNYGPSADTPPAYWQDQSVRPFAQVKDHVLLPRATRLAEVDAVFREV
                     LTPAVIEGIVGLVPDAWLTGGLWTESPAEVRKVYSEFLIRRVANSAVFIEYAVDAQKK
                     NNASV"
     gene            complement(4282..4611)
                     /locus_tag="VKQ52_11935"
     CDS             complement(4282..4611)
                     /locus_tag="VKQ52_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87950.1"
                     /translation="MKMLLFTALAVSLTTAAFAQGPGHQPTHIPGHPRVNQVNGRIDN
                     QERRINTERREGELTGGQARQDRRNLRAINQEKHDMRSQDNGHLTRGDQRALNQQLNR
                     NSRKIGQ"
     gene            4700..>4905
                     /locus_tag="VKQ52_11940"
     CDS             4700..>4905
                     /locus_tag="VKQ52_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ87951.1"
                     /translation="MEFLEVRLPDISFTLKKNQRLAIAGETGSGKSTLLKIIAGLATP
                     NAGPQTSAAANSANPNIGPQTSPAT"
BASE COUNT         1070 a         1613 c         1165 g         1057 t
ORIGIN      
        1 tttgtcgtgt tcttactatc gaaattcatg aactggaccc cgctgccgat attcacattc
       61 agcttcgcat gctgtaaccg gtaattcaga ctgaccctgt cgctgttgga ttcgaatctg
      121 tacgagttac tgaacaagct atcgaaggaa gtatacccct tcgccgcgtc gctgtagtca
      181 taggtattat tgatcgtccg gctccggtta tacgtatggt tataattcac ctcgatgatc
      241 gaatgcttcg atatcggttc cgtatacgat agggtagggg cgatattcac cgaatgcagc
      301 gaatcggtat aaaactgccg taaatcctgc aacgagtcca acttgtagaa agtattcgcg
      361 gaatgataat agccggaccc gtcattcaca ttcaccgttc cggtcacatc cagcgatatc
      421 gtccggtacg gcttccggaa tttgtgccgc aacgtcaggt tgctcccgtt gatgttgaac
      481 ccgtcattgc tgctatacga attgccgatg ctgctgctca cggggcgccc gtcctggtcc
      541 accgtcgttg tcgtctgact gctgttcggg ctcgtatgct gccacgtgat attcggccgg
      601 aagacaaagg aattcccgct atcgttctta ttctcgatat tgaagttcag ccgttgattg
      661 gtgctgcggg agatcgccgg gctcgtctgc gtgatactat tcgacgtatc ctccgccgtc
      721 gtaaagaatt tctgcgtaag actcgaggta ttcgacgata catgcgtgaa attgtagaag
      781 tagctgccat aagcgtcata cttcccccag ctgtcccggt aattggctcc gccggcccat
      841 acggtcgtga tccccgccgc gctgggattg gccgccgcac ccggcccccc gccgcttccc
      901 cgccggctcc cgctgctccc cagtacatcc tgaacggtaa agttctgctt attgatatca
      961 ttcccctcct cgagcgccga tatctgccgg tcgttgttga aatgatgcag caccgcgctc
     1021 tcgttatagg tctgatccgt acccgcgccc gcgatcccgc ggccaaaata gccctggcgc
     1081 ttgtccttcc gcgtgatgat attgatcgtc ttgatccggt tcccatcgtc gaaacccgag
     1141 aacttgctct ggtcgtcgag atcatcgaag atctggtact tctcgatgat ctccggcggg
     1201 atattccgcg tggccaattt ggggtcgttc ccgaaaaatc gtttgccgtt aacgagcaat
     1261 cgctgcacct tttccccctg cgccgtgata ttcccgctgg catccacctg taccccgggc
     1321 aacttcttca gctgatcctc ggccaccgca ttgggcttcg tcgcaaaact ctcggcatta
     1381 tactcgatcg tatccttttt tatcgtcatg ggtggacgtt ggatgaccac ctccgccagg
     1441 atgtccggcg cgcgctccat caggacggct ccgagatcga tgtccctggt cgtcccgtcg
     1501 atgacaaaat tcttccggac atgccggtat ccctcgaacg tgatgaccag gtggtacccg
     1561 ccggtcgcca gtcccctgaa caaaaaacca ccccgcttat ccgtgatcgc gaactgggca
     1621 tccgatgtat cccgctcggg ggtaacactt accgtcgcat cgctcatggg cagtttaccc
     1681 gccgtgtcgg caacgatccc ccgcaccgac ccccggatcc gcacctggga atgggcggaa
     1741 agaaaaaaaa aggcgacgaa caggggggcc aggataaaag gcttgctcat aagctgggga
     1801 attacccgca tcggcaacgg acgaagatcc gcgcccggtc cgggggctca gtgactacga
     1861 aattaatcgc aggtttactc cccaacggcg atttcaggtg aatacccact ctttatcggt
     1921 ggatggacga aactccttaa cttgccgtca tgaaagcgct caacatcctg gcattatttc
     1981 taagcattgg gaccgcaggc cacgcccagt tcgaaggcgt cctgacctat gactgcacta
     2041 tcaggaacaa aaccctcacc acggtctatg aatccaggac gaaggtcttg ctggaggcga
     2101 agatctaccc catgaaggcg gggatcgccg atatccggga ggcccgggaa caagacccga
     2161 tcctcttcga tttcgaggcg aaaaaggcga ctcgcatcgg cgcaaaacac cacgagacca
     2221 tttcctccga cctggcgccg gtgacaaacg accataatga taaagcaaaa gacggggata
     2281 taacgatcac gttggtgggc ccggaaaaga tcgatgccta caactgccag cacttcgtcg
     2341 taaaaataaa gaacaccacc accgagctct ggatcacgaa agaccttggc gtctccgaga
     2401 tctgtatgct ctcccagttc gactactatc ctgccggctc tatcctctat gataagctca
     2461 aagccgcggg cggcgacggc atcgtcgttc gttcaaagac cggcgaggtc aacgtcaatc
     2521 tcaccaacgc ccaggtaaag accgtgccgc catcctactt cgagatcccc ggcaaaccct
     2581 gatcacggca tcgcgccaaa tcctcatcgc catgaaccct gacgcgggcg ccccctcatg
     2641 ggccgggctc tccactatag tcctcgccct ggctaccgcc ggctgcgggc tgccgctgcg
     2701 atccctggcg cggcctcgcg ccaaatcctg atccgcgccc ctcacagacc tctcgtcgcg
     2761 ccctctcgtc cggcccccgg ctcatagcta tagaaccatc gtttcgtata gtcgctgtaa
     2821 ctcccccgca aaatctccgc aaaaccccgg atgaacccgc gacgtctgga tgatcgtgct
     2881 cctcgtcgcc gtcagccagc gaaaccgccc cgctccgtcc agtttcccga tcggcccacc
     2941 cgcctcccca ccgttcacga tctcctcgaa ccctttcaga tgcgcctcca cctccgcgat
     3001 atccagcgtc ggcgccaagg tccgcaaccg ttcctcatcc acacgatact tcaaccccaa
     3061 cgcccgcgag ccatgacaat acaggatcac ccccacattg aggaactcct cccggtctac
     3121 acgcgggacg acccggatca cagcgtactc gaacaattgc ttttcttgca ttgagggcct
     3181 catttaaacc gatgcgttat tttttttctg cgcatccacg gcatattcta taaagacggc
     3241 ggagttggcc acccgcctga tcaaaaactc gctatacacc ttacgcacct ccgccggcga
     3301 ttccgtccat aacccaccgg tcagccaggc atccggcacc agcccgacga tcccttcgat
     3361 caccgccggt gtcaacacct cgcggaacac ggcatccact tcggccaatc gcgtcgcccg
     3421 gggcaacaac acatgatcct tcacctgcgc aaacggccgc acagattgat cttgccaata
     3481 cgccggaggc gtatcggccg aaggcccata attagattca tcccaactat gatgaaaata
     3541 aaggctcgct ccgtgatcga tcagccacaa ttccttattc cagaccagca tattcgtatt
     3601 ccgcgccgtc cggtctacat tcgtcagcaa acaatccagc cacacgatct gtgaagccat
     3661 cgtctcgtcc acccggttga ccacggggtc gaacgtgatg ctcccctgca ggtaatgcag
     3721 gcccaggttc aaccccacac tggcctttaa cagatcctgg atctcctcat ccgcctcgct
     3781 tctcccgaac gcggcatcga gctgcgcaaa cacgatctcg ggtatccgta atcccagcgc
     3841 ccgggcaatc tctccgccga tcagctccgc tatcagcgat ttcacgccct gccccgcacc
     3901 ccggaatttc aggacataca ggaacccatc atcagcctcg gctatcgccg gcaacgaacc
     3961 gccctccctc aaaggggtga cataccgcgt aacggccacc tgtcgcagct caggaataga
     4021 aatagacata acgaagcggc aagataagtc gaatttcgat attcccattc cccacaccgc
     4081 gacgccatca taacgcctcg catcggccat cctcatgccc cccacatcgt gatggcctca
     4141 tcccactcca caccgcgacc ccgccaaagc gcccccggcc cggcgcaaaa aaaactgcgc
     4201 cggaaccgct tcctcaaaga gagcgatcac ccggcgcagg cggaggacta tatccttcaa
     4261 acgatacgat caccaactgg cctattggcc gatctttctg ctgttacgat ttaattgttg
     4321 attcaatgcc cgctgatcgc cacgggtcag atgtccatta tcctggctcc gcatatcatg
     4381 tttctcctgg ttgatcgccc ggagattccg ccggtcctgg cgcgcctgac cgccggtcag
     4441 ctcaccttcc ctgcgttcgg tgttgatcct tctctcctgg ttatcgatgc ggccgttaac
     4501 ctggttgaca cgcgggtgac cgggaatatg cgtaggctga tggccgggac cctgggcaaa
     4561 agctgccgtt gtaagcgata cggcaagcgc cgtgaatagt agcattttca ttttgtaaga
     4621 ttttaagttg catatttgaa cggccccggt ccggaaggtt taacctaaaa atggttaatt
     4681 gtggttaatt ttgagcccaa tggaattcct cgaagtaaga ctacccgata tctccttcac
     4741 cctcaagaag aaccaacgcc tcgccatcgc cggcgaaaca ggctccggca aaagcaccct
     4801 gctgaagatc atcgcgggcc tcgctacccc caacgcgggc cctcaaacct ccgccgccgc
     4861 aaactccgcc aaccccaaca tcggccctca aacctccccc gccac
//
LOCUS       DATGFG010000637         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_33383, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000637 DATGFG010000000
VERSION     DATGFG010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33383"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VKQ52_11945"
     CDS             complement(<1..353)
                     /locus_tag="VKQ52_11945"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HLZ87952.1"
                     /translation="MIRAICCVPVSPLRAEPSHRTEMVSQLIFGECCEILEQTREWSR
                     VRVRYDGYEGWCTGTHLTEIDESEYDTGVTPLVPEWVTPLEYNGHPMMVPMGSHLTAM
                     KNGRAHWRKNVVHYKG"
     gene            506..1084
                     /locus_tag="VKQ52_11950"
     CDS             506..1084
                     /locus_tag="VKQ52_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ87953.1"
                     /translation="MNKDPFHHMTDQELLNRFYDDHNNEWLGSLLQRYTLLLFGVCMK
                     YLRDEEEARDCVQHIFVKVITELGKYRVEYFKSWIYTIARNHCLMKLRDQHGRQTELS
                     DSLLAAWDEEPGKNLHWEKEQLLQWMGQALEELGKEQKLCVILFYLEKKSYQEIATTT
                     GFTLMQVKSYIQNGKRNLKLSIEKKAREGANK"
     gene            1081..1443
                     /locus_tag="VKQ52_11955"
     CDS             1081..1443
                     /locus_tag="VKQ52_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87954.1"
                     /translation="MNKDWLKILSNSNKDIDNQQLMDYLSGKLSGEALHEMERSMADS
                     EFLNDAVEGLQRVGDKKNMQAYVDELNASMQKTLEKKKNRRLKRRLREDPWALIAIVL
                     VISLCILGYIVIRKLLHP"
     gene            1491..>2143
                     /locus_tag="VKQ52_11960"
     CDS             1491..>2143
                     /locus_tag="VKQ52_11960"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87955.1"
                     /translation="MDVKRLFTLLSLFCILQSANAQRISYIVSFPNLAHHEAEVELVA
                     SDLTNRTAVFRMSRSSPGRYATHEFGKNVYDVRAFDQSGKPLPVNRTDGDVYEVTRHT
                     GYIRVQYTLYGNYADGTYVGLDPTNVHFNMPGAFLWLKGAENVPITIHFNVPAEWHWT
                     VATQLQPTGDSLTFLARNFQYFMDCPTKIGALRYRKWEVKNPDGKTYTFRIALDGGA"
BASE COUNT          565 a          658 c          467 g          453 t
ORIGIN      
        1 cccttgtagt gtacgacatt cttacgccaa tgcgcacgtc cattcttcat cgccgtcaga
       61 tgactgccca tcggcaccat catcggatgc ccgttatatt caagaggagt gacccattcg
      121 gggaccagcg gcgttacccc ggtgtcatat tcgctctcat cgatctccgt cagatgagta
      181 ccggtacacc agccttcata accgtcatac ctgacccgga ccctgctcca ttcccttgtc
      241 tgctccaata tctcgcaaca ctcgccaaag atcagctgac taaccatttc tgtccggtgt
      301 gagggttccg ccctgagggg gcttacgggg acgcaacaaa ttgctctgat catacggtaa
      361 attaaaaaag gtttcgccga ctgcaaaatt ttatcctgtt aagatcaacc cactacttaa
      421 aacataaaaa tttaatgtaa attttacaga cttatgcaat ttcagccgcc ctgcatctat
      481 agtatataat gcttattttc cgcccgtgaa caaagatcca ttccaccata tgaccgacca
      541 ggagttgctg aaccgtttct atgacgatca taacaacgaa tggctgggtt ccctgctcca
      601 acgctatacc ctgcttcttt tcggggtctg catgaaatac cttcgggacg aagaagaagc
      661 ccgcgactgc gtccagcata tcttcgtaaa ggtgatcacc gaactgggca aataccgggt
      721 cgaatatttc aaatcctgga tctataccat cgcccgcaac cactgcctga tgaaactgcg
      781 cgaccagcac ggccggcaga ccgaactctc cgactccctc ctcgccgcct gggacgaaga
      841 acccggcaaa aacctccact gggaaaagga acagctgctc caatggatgg gccaggccct
      901 cgaagaatta ggaaaagagc aaaaactctg tgtaattttg ttctacctgg aaaaaaagag
      961 ttaccaggaa atagccacga ccactggctt tacgttaatg caggtaaaaa gctatatcca
     1021 gaacggcaag cgaaacctaa aactatcgat cgaaaaaaaa gcccgggagg gcgccaataa
     1081 atgaacaagg actggctcaa gatattatcc aacagtaaca aggacatcga caaccaacag
     1141 ttgatggact acctcagcgg caaactctcc ggtgaagccc tgcatgaaat ggagcggtcc
     1201 atggccgata gcgaattcct caatgacgcc gtcgaaggcc tccagcgggt aggcgacaaa
     1261 aagaatatgc aggcctatgt cgacgaactg aatgcgtcca tgcaaaaaac cctggaaaaa
     1321 aagaagaacc gccgcctgaa acgccgcctc agggaagatc cctgggccct gatcgccatc
     1381 gtcctcgtga tcagcctctg cattcttggc tatatcgtca tccgaaagct ccttcatccc
     1441 tgacaaagtc ccgtaaattg cctatattcg tctaactatt agcagtttat atggatgtaa
     1501 aacgcctttt cacccttctt tccctgttct gtatcctcca gtccgccaac gcccaacgca
     1561 tcagctatat cgtgtccttt cccaacctgg cccaccacga agccgaggtc gagctggttg
     1621 ccagtgacct caccaaccgg accgcggtct tccggatgag ccgctcctcc cccggccgct
     1681 atgcaaccca cgagttcggc aagaatgtct acgacgtcag ggccttcgac caatccggca
     1741 aacccctgcc cgtcaaccgc accgacggcg atgtctacga agtcacccgc cacaccggct
     1801 atatccgggt acaatatacc ctctacggca attacgccga tggcacgtat gttggcctcg
     1861 accccaccaa cgttcatttc aatatgcccg gcgccttcct ctggctgaaa ggcgcggaga
     1921 acgtccccat taccatccat tttaatgtgc ccgcggaatg gcactggacc gtcgccacac
     1981 aactgcaacc cacaggggac agcctcacct tccttgcccg caatttccag tacttcatgg
     2041 actgcccgac aaagatcggc gccctccgct accggaaatg ggaggtaaag aaccccgacg
     2101 gcaagaccta caccttccgc atcgccctcg acggcggcgc cga
//
LOCUS       DATGFG010000638         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1494979, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000638 DATGFG010000000
VERSION     DATGFG010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494979"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VKQ52_11965"
     CDS             complement(<1..533)
                     /locus_tag="VKQ52_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87956.1"
                     /translation="MIAISTRKITPEERKLVTKAKPSLYRRIESFFMTMGFIVLMLVI
                     PLLIYDHFRPVSSGTQAVYCIFIVTLALFISIWIFRGRLGYQKKRRSDPEEVEVIRVQ
                     TTRAVRREDPEDFGVAFYIDVMDMGRQKVLYLWGQYLDILEYEKEFPNTEFEIVRFAG
                     RDSFIDFALLGVHFPEE"
     gene            complement(552..1361)
                     /locus_tag="VKQ52_11970"
     CDS             complement(552..1361)
                     /locus_tag="VKQ52_11970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ87957.1"
                     /translation="MNLHNKNAIIYGAGGSLGGAVARAFAQAGARVFLTGRGAGSIQK
                     ITDEILAAGGVAEAAVVDAFDKDAIGKHLEMVVEKAGSVDISFNAVGVDVVQNMPLID
                     ISMEDFVTPVTRTMQTRFLTATLTARVMIRQRSGVILSLTATPGGIGYPHTGGFAPAC
                     CAVEGFSRNLASELGIYGIRVVNIRSGGSPDSKVFRDAIDRFPDAMETILRQMEGDTM
                     LKKLPSMAYIANTAVFLASDLAGMITGVTVDVTCGTTAGLNYRVASPDVVG"
     gene            1434..>2056
                     /locus_tag="VKQ52_11975"
     CDS             1434..>2056
                     /locus_tag="VKQ52_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87958.1"
                     /translation="MNKKIKTIITGATGMVGEGVLLECLDHPDVEQVLVINRKPGGRT
                     HPKLREIIHPDFFNLQPIEAQLAGYNACFFCLGISSVGIGKEEYKRITYDLTLHAGQV
                     LAGQNPDMTFCYVTGAGTDSSEKGRVAWARVKGATENALMRLFKNAYMFRPAFMKATA
                     GQKNLKGWYKYFAWLYPIGHAIYPAGFCTLREVGQAMINVAARGYPK"
BASE COUNT          488 a          586 c          539 g          443 t
ORIGIN      
        1 ttctcttcgg ggaaatggac gcccagcaac gcaaagtcga tgaacgaatc ccggccggca
       61 aaccggacga tctcgaattc ggtgttgggg aactcctttt catattccag tatatccagg
      121 tattgccccc atagatacaa gaccttttgt cggcccatgt ccattacgtc gatatagaag
      181 gcaactccaa aatcttcggg atcttcccgc ctgaccgctc ttgtcgtttg gacccggatg
      241 acttccactt cttcgggatc actcctgcgc ttcttttggt agccgagcct tccccggaat
      301 atccaaatgg aaatgaatag cgcaagggtc acgataaaga tacagtagac agcctgtgtg
      361 ccggaggaaa ccggcctgaa atgatcgtat atcagtaagg gtatcactaa catgagaacg
      421 ataaagccca ttgtcatgaa aaagctctcg atccggcggt acaagctggg ttttgctttg
      481 gtgacgagtt ttctttcttc cggcgttatc tttctggtcg aaatggcgat catgcccgct
      541 ttgttttaca gttaccccac cacatcgggc gaagccaccc ggtaatttaa ccccgcggtc
      601 gtaccgcagg tgacgtcaac ggttacgccg gtgatcatcc ccgccaggtc cgaggccagg
      661 aaaacggcgg tattggcgat ataggccatc gagggaagct tcttcagcat cgtatcgccc
      721 tccatttgcc ggaggatcgt ttccatggca tccggaaagc gatcgatcgc atccctgaac
      781 acctttgaat cgggcgagcc gcccgaccgg atattgacca cccggatacc atagatgccc
      841 agttccgaag caaggttccg ggaaaaaccc tccacggcgc aacaggccgg cgcaaaacca
      901 cccgtatggg gataaccgat ccccccgggc gtcgcggtaa gcgagaggat cacccccgat
      961 cgctgcctga tcatcaccct cgcggtaagc gtggccgtca ggaaccgggt ttgcatggtc
     1021 cgggtgaccg gggtcacgaa atcttccatg gagatatcga tcagcggcat attctgtacc
     1081 acatcaaccc ccaccgcatt aaaggagatg tccacgctcc cggccttctc caccaccatt
     1141 tccaggtgct tgccaatggc atccttatca aaggcgtcca ccaccgctgc ttccgcgacg
     1201 ccgccggcgg caaggatctc atcggttatt ttctgaatgg aaccggcgcc gcgccccgtg
     1261 agaaagaccc gggctccggc ctgggcaaac gccctggcaa cggcgccgcc aagggaaccg
     1321 ccggcaccgt agataatggc attcttgttg tgaaggttca tatcgctttt ttcaagatta
     1381 aggttatctt caaccgttgt taaacagatt gaaaataccc aattgcaggc aaaatgaata
     1441 aaaaaattaa aaccatcatc accggtgcaa cgggcatggt cggagaaggg gtgctgctcg
     1501 aatgcctgga ccaccccgat gtggaacagg tgctggtcat caaccggaag cccggtggaa
     1561 gaacgcatcc caaactgcgg gagatcattc atccggattt ttttaacctc caaccgattg
     1621 aagcccaatt ggcggggtac aatgcctgct ttttttgcct ggggatatct tccgtcggca
     1681 tcggtaaaga ggaatataag cgcatcacct acgacctcac cttgcacgcc gggcaggtgc
     1741 tggcagggca gaatcccgat atgacctttt gctatgtcac gggcgcgggc accgacagca
     1801 gcgaaaaagg ccgcgtcgcc tgggcccgcg taaaaggcgc caccgagaat gccctgatgc
     1861 ggctgtttaa aaatgcgtat atgttccggc cggcgtttat gaaggccacc gcgggacaaa
     1921 aaaatctaaa aggctggtat aagtattttg cctggctcta tcctatcggc cacgctattt
     1981 atccggccgg cttttgtacc ctccgggaag tggggcaggc catgatcaac gtggccgcca
     2041 gggggtaccc aaaaca
//
LOCUS       DATGFG010000639         4454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_709861, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000639 DATGFG010000000
VERSION     DATGFG010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709861"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VKQ52_11980"
     CDS             <1..943
                     /locus_tag="VKQ52_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLZ87959.1"
                     /translation="GKYTAEDVVVTAGCMEALVMCLKATTRPGDTVAIENPTYFGIFQ
                     VIESLGLKAVEIAADPLTGIDPKKLESLIRKFQIKACVFVTNFSNPLGSCMPDEKKKA
                     LVQLLARHRIPLIEDDIYGEMYFGRNRPRTCKSFDTEGWVLYCSSLSKSLAPGYRIGW
                     TIPGRFTQRLIQIKMMHTVSGTTLTQQAIGHFLGIGRYEYHLKKLRKALHTQCLRYMQ
                     GILQYFPPDTKVSRPQGGFVLWIQLDHQLNAYRLYQEALKHNVSVAPGQLFSAKGQFG
                     NCLRLSYARPWDAEVEEALRMLGSLIRKMSPTPRPAT"
     gene            988..1428
                     /locus_tag="VKQ52_11985"
     CDS             988..1428
                     /locus_tag="VKQ52_11985"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HLZ87960.1"
                     /translation="MNLVQRVEHWGDTHHPQWLDVIRILLGAFLCFKGIDFLMNMGTM
                     LGLITNRMSFGSFTSMLMSNYIAFAHLLGGILLILGVLTRFACTLQIPILLGAIFFLN
                     TSLYRPFSEMAIAILVLLLLILFLIVGNGPIQLLKFRDEKTDSI"
     gene            complement(1432..2502)
                     /locus_tag="VKQ52_11990"
     CDS             complement(1432..2502)
                     /locus_tag="VKQ52_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87961.1"
                     /translation="MRTDWMVTALLFAVTVAAAQRPIGSGEALATAHRIEAATNRGDA
                     GALDRFLDLDSLIDRMRQKSHALKDPAFFRGFSESFTTNFGHYGQRILPSIKLGNYRL
                     LREYESKGSRHLLFRMFGAGGLNYHDYTLIRVKDSIKAADVYVYSIDESMSGSIATLA
                     DMMNNSAEKLPEDVSMMIKLTDENNKKNYAGVKEQYEQLDEKYKKTKAMQLVYISACH
                     HLDQALYEGALEHFAASFPDAPSGYLMLIDLYYLRKEYDKGFTALDKMDKLLGGDPAL
                     DIFRGNFYRLQGKKAESLACYERVFRYDPGIAANARFLIVAYEEAGDKAKAKTVITEF
                     RKTGAYHEADLSDLFTKYPELK"
     gene            complement(2502..4280)
                     /locus_tag="VKQ52_11995"
     CDS             complement(2502..4280)
                     /locus_tag="VKQ52_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HLZ87962.1"
                     /translation="MTEPTRLFDCLTLNLEKDPLPDMLAGKEGGVYRTYSTQEVADTI
                     QKLSTGLLRLGVSGVDRTPEGVEKIGIVCKNRPEWIMLDMAVQRTGAVLAPVYPTIAL
                     HELEFILNEAQVKIIFVNDRDLFEKVESLRDRVPTLREVFTIEPVAGARNWKELLVTP
                     TAEEVARLKQIQDSIDEKDLATVIYTSGTTGTPKGVMLSHKNIVSNVKSCIPCLPQGK
                     IRALSFLPLNHIYERMLSYVYLFNNSSIYYAESMDTIGDNLKEVKPQMFVTVPRLLEK
                     VYDRIMHKGAELTGVKKKLFFWAHDLAARYEINKPMGFWYDLQLSLANKLIFSKWREA
                     LGNNITCIASGGAAAQVRLIRIFTAARVPILEGYGLTETSPVISVNRMDIKDRHFGTV
                     GPLIPDVEVRIAEDGEILCKGPNVMMGYYKRPDLTAEAITDGWFHTGDIGEFVDNKYL
                     KITDRKKEMFKTSGGKYVAPLAIESKLKESPFVEQIMVVGPERKFVGALIVPAFQQLT
                     EWSRQNGVTASSHDQMIRDPKVLALYRQVIDGYNSNFGHVEQIKRFELLPAEWTVETG
                     EMTPKLSLRRKVVTEKYAATIEKIYA"
BASE COUNT          993 a         1405 c         1047 g         1009 t
ORIGIN      
        1 tggaaaatat acggcggaag acgtcgtcgt aacagccggc tgcatggaag cgctggtcat
       61 gtgcctgaaa gcaacgaccc ggcccgggga taccgtggcc atcgagaacc ctacctattt
      121 cggcatcttt caggtgatag aaagcctcgg cctgaaagcc gtggagatcg ccgccgaccc
      181 attgacgggt atcgacccaa aaaaactgga gagcctgatc cggaaattcc agatcaaagc
      241 ctgcgtcttc gtcacaaatt tcagcaaccc cctgggctcc tgcatgcccg acgaaaaaaa
      301 gaaagcgctg gtgcagctgc tcgcccgcca ccgcatcccc ctcatcgagg acgatatcta
      361 tggagaaatg tatttcggcc gcaaccggcc ccgcacctgt aaatccttcg acaccgaagg
      421 atgggtattg tattgcagct ccctgtcaaa atccctggcc ccgggctatc gtatcggctg
      481 gaccatcccc ggccgcttca cccaacgact gatacagata aaaatgatgc acaccgtgtc
      541 cggcaccacc ctgacccagc aggccatcgg ccattttctc ggcatcggcc gctacgaata
      601 ccatttgaag aaattgcgca aagcccttca cacccagtgc ctgcgctaca tgcagggcat
      661 cctccaatat ttcccgcccg acacgaaggt atcccggccc cagggcggct tcgtactgtg
      721 gatccagctg gaccaccagt taaatgccta tcgcctctat caggaagcat tgaaacacaa
      781 tgtctccgtg gcgccgggcc agcttttctc cgcaaaagga caattcggca actgcctccg
      841 gctcagctac gcccgcccct gggacgccga agtggaagaa gcgctccgca tgctgggctc
      901 cctgatccgc aaaatgtcac ccaccccacg cccggctact tgacgatggc ataaaaattt
      961 cgtaccttat aggtaaaaca atgctatatg aatctagtac aacgtgtaga acactggggc
     1021 gacacccatc atccccaatg gctcgatgtg atccgcatac tcctgggtgc cttcctttgc
     1081 ttcaaaggca ttgacttcct gatgaacatg ggcaccatgc tcgggctgat caccaaccga
     1141 atgtcctttg gttccttcac ctccatgctc atgagcaatt atatcgcctt cgcacacctg
     1201 ctcggcggta tcctcctcat cctcggcgtg ctgacccgct tcgcctgcac gctgcagatc
     1261 cctatcctgt tgggcgctat attttttctg aacacctccc tctaccggcc cttctccgaa
     1321 atggccatcg ccatcctcgt actcctgttg ctgatcctgt tcctgatcgt cgggaatgga
     1381 cccattcaac tgctgaaatt ccgcgacgag aaaacggatt ccatctgacc gctattttag
     1441 ttcgggatat tttgtaaaaa gatcgctgag gtcggcttcg tgataggcgc cggtcttccg
     1501 gaattcggtg atcaccgtct ttgcctttgc tttgtccccc gcctcctcat acgccacgat
     1561 caggaagcgc gcgttggcgg caataccggg atcatagcgg aacacccttt cataacaggc
     1621 aagactctcc gccttcttac cctgcagccg gtagaaattg cccctgaaga tatcgagggc
     1681 ggggtcccca cccaggagct tatccatttt atcgagggcc gtaaaaccct tgtcatattc
     1741 tttccgcaga taataaagat cgatcagcat caggtaacca ctcggggcat ccgggaaact
     1801 ggcggcaaaa tgctcgagcg caccctcata caaggcctga tcgagatgat gacaggccga
     1861 aatatagacc agttgcatcg ccttggtctt tttgtacttc tcatccagct gctcatactg
     1921 ctccttcacc ccggcatagt tcttcttgtt gttctcatcg gtcagcttga tcatcatgct
     1981 gacatcctcc ggcagttttt cggcgctgtt gttcatcata tccgccaggg tcgcaatact
     2041 gccgctcatc gattcatcga tcgagtagac atacacatcg gccgccttga tcgaatcctt
     2101 cacccggata agcgtatagt catgatagtt taaccccccg gccccgaaca tccggaaaag
     2161 taaatgacgc gaacccttgc tctcatactc ccgcaaaagc cggtaattac ccagtttgat
     2221 actgggcagg atccgctgcc cataatgccc gaaattggtg gtaaaagact cggaaaaccc
     2281 ccggaaaaag gccgggtcct tcaacgcgtg gctcttttgc cgcatccggt cgataagact
     2341 gtcaagatcg agaaaacggt cgagcgcacc cgcatccccc ctattggtgg ccgcctcgat
     2401 ccggtgggcc gtggccaacg cctccccgct gccgatcggc cgctgcgccg ccgctacggt
     2461 aacggcaaaa agcaatgccg tcaccatcca atccgtcctc attatgcgta aattttttcg
     2521 atggtcgccg catacttctc cgtcaccacc ttgcgccgca gacttagctt gggcgtcatc
     2581 tcacccgtct ccaccgtcca ctcggccggc aacagctcga agcgttttat ctgttccaca
     2641 tgtccgaaat tgctgttata cccatcgatc acctggcggt aaagggccag caccttcggg
     2701 tccctgatca tctggtcatg gctgctcgcc gtaaccccgt tctgccggct ccattccgtc
     2761 agctgctgaa aggcagggac gataagcgcg ccgacaaact tccgctccgg cccgacgacc
     2821 atgatctgct ccacaaaagg cgattccttc agcttgcttt caatggccag cggggccaca
     2881 tacttcccgc cgctggtctt gaacatctcc ttcttccggt cagtgatctt caggtattta
     2941 ttgtctacga actccccgat atcccccgtg tggaaccagc cgtcggtgat ggcttcagcg
     3001 gtaagatcgg gtcgtttata atatcccatc atcacattcg ggcccttgca gaggatctcg
     3061 ccatcctcgg cgatccttac ctcgacatcg gggatcagcg gaccaaccgt accaaaatgc
     3121 cggtccttta tatccatccg gttgacacta atgacgggag aagtttccgt aagcccatag
     3181 ccctccagga tcgggacacg cgcagccgtg aagatgcgga tcagccgcac ctgggccgcc
     3241 gccccgccac tggcgataca ggtaatattg ttgccaagcg cctcgcgcca tttgctaaag
     3301 atgagcttat tggccagtga aagctgaaga tcataccaaa atcccatcgg cttgtttatc
     3361 tcataccgcg cagccaggtc gtgggcccag aagaacagct tcttcttcac tcccgtgagc
     3421 tcggccccct tgtgcatgat ccggtcgtac accttctcca gcagccgcgg aacagtgaca
     3481 aacatctgcg gcttcacctc cttcaggttg tccccgatgg tatccatact ctcggcataa
     3541 taaatggaac tgttgttgaa taggtacaca tacgacaaca tccgctcata gatatgattc
     3601 agtggcagaa aactgagcgc cctgatcttg ccctgtggta aacaggggat acaggatttg
     3661 acgttgctca cgatattctt atgcgaaagc attaccccct tcggcgtccc cgtggttccg
     3721 gaagtataga taacggtggc cagatctttc tcgtcgatgg aatcctgtat ctgtttcagc
     3781 ctcgccacct cttccgccgt cggcgttaca agcagctcct tccagttccg ggccccggct
     3841 accggctcga tagtaaacac ctcccggagg gtgggaaccc ggtcccgaag actctccacc
     3901 ttttcgaaaa ggtcccggtc attgacaaaa atgatcttca cctgggcttc gttcaggatg
     3961 aattcgagct catgcagcgc gatggtggga taaacgggtg cgaggacggc gccggtacgc
     4021 tgtacggcca tgtcaagcat gatccattcc ggtcggttct tacatacgat gcctattttt
     4081 tctactcctt ccggggtccg gtctactccg gaaaccccca gccgaagcaa tccggtactg
     4141 agcttttgaa tagtgtcggc aacttcctgc gtgctatagg tccgatagac cccgccttcc
     4201 tttcccgcca acatatccgg cagcgggtcc ttctccaggt tcagggtcag acaatcaaac
     4261 aatcgcgtgg gttcggtcat aaataactgt ttgccgacaa attagcgaaa aaagcacgaa
     4321 tggccaaccc accggcgaca ccccgaaacc taagccggcc acaccccaaa acgtaagccg
     4381 gcgacacccc aaaacctact tcgccgccac ctggtaatac tgcccctccg gcatcttgaa
     4441 gaccctccca tgct
//
LOCUS       DATGFG010000640         6199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1500250, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000640 DATGFG010000000
VERSION     DATGFG010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500250"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VKQ52_12000"
     CDS             complement(<1..1304)
                     /locus_tag="VKQ52_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HLZ87963.1"
                     /translation="MIRTLDIVVSILYILAILAIGLYVGIRHRRKNAGEYFLAGKTLR
                     WPAIGLALFATNISTVHLVSLAQSGFDTGLLNGDFEWMAAFTLILLALFFVPFYITSG
                     VTTLPDFLEKRYDRSSRDWLVMVSIVSAVIIHIAFSMLAGGIVLKTLFGVSMYTSVVV
                     ISLITALYTIIGGLKAVVVTESIQTVVLITGAVIISMAAWHRMGGWHAMTDTLHAHGQ
                     INRLSMLRPAGDSSGMPWYSVFLGYPVLGIWYWCADQTIVQRVLGARDEQHARIGPLF
                     CGFIKILPVFIFVLPGLFAYTLAATGRLDIHSLRTAGGDVNSKGIYTLMITQLLPSGL
                     VGILVAALLSGLMGQVSGALNSISTLVSYDIYRRRRPEADDRQLVRMGRISAGMALLL
                     SLALLPLLNRYESIFNGLNDIIAHIAPPITCVFLLGVFWKKAS"
     gene            complement(1309..2769)
                     /locus_tag="VKQ52_12005"
     CDS             complement(1309..2769)
                     /locus_tag="VKQ52_12005"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLZ87964.1"
                     /translation="MKHGLLTAALLVAINLPAQETTSSSGKASALIRGQVKNNRDTSL
                     GYRIYGYLRDPQESIAIDRKGRFSKEIPIRPGGQEMTLAVGYNLFIFIQPGDTIDLSW
                     DGKDAGPSLSVSSPKAERDTELKTQLFLYQQFQPRYRDIIVSYSRKLPDSVNFNKIDH
                     LYNDEITALLTRPRTTHTNKFIEDVYYQYATFLADHFLLTGHDLSLEHPYEGTDLPFL
                     ARQDQWHALRPYQAESEETFRTSHWYREFLLEYIKFGFTPFMTWDSKDAIHYLQNDYY
                     NGMRSFRSREIRDWFVTRLIMDAPDFFSFDEIDTVYRDFITKVTFRPYADTLRQFYAG
                     LQKLRSGNPAPAFVLQNEKGGNVSLADLRGHVVYIDFWGNDCAPCIDDIKNHVPALHE
                     RYKDKNIVFVNICVDSDEKTWKEGLEKLHLEGVNLVAEGWTNNKVCNDYQVRGIPHYI
                     LIDAAGRIVDSNAPRPWQNELFPLLDTLVEKQPAYQ"
     gene            complement(2766..3995)
                     /locus_tag="VKQ52_12010"
     CDS             complement(2766..3995)
                     /locus_tag="VKQ52_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLZ87965.1"
                     /translation="MIKSILVKDLRFPLAKGAGSDAIHRDPVYSYAVTQLQDDSGHTG
                     TGLAFTLGEGNDMVCEAARFYARRLLERDLEELMAGFGREFNALSNEQQFRWLGPHKG
                     VVHLGLASVTNACFDLWAKKRGLPLWKLLIDLSPEELLNTLDLSYLEDVLPSPRALQL
                     LREKVPGKVSREKILEKGYPGYDTSIGWFNYSDEQVRENTIRAVAEGFTAMKLKVGSA
                     DPTRDIRRANIVREAAGADIRIMVDANQQWTLPQALRIGKHLQPMDPFWIEEPTHPDD
                     VLAHKTLAEALAPTRLAIGEHVPNRIVFKNYLQAGAAGFIQVDAVRVGGVSEFITVSL
                     LSRLFGIPVVPHVGDMGQLHQHLVLFNHISLGHEALFLEHIPHLKKYFQHPADVRGGV
                     YATPQEPGSSCDLIDGL"
     gene            complement(4069..>6199)
                     /locus_tag="VKQ52_12015"
     CDS             complement(4069..>6199)
                     /locus_tag="VKQ52_12015"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HLZ87966.1"
                     /translation="YLLEGKNDSAQALVDKSFICTGPGSGGTRYGCFQMLGNLHLQFD
                     YEASDGVRAKDSPAFDHYSRELILHDAMARCTYKVAGVTYKREYLTSFGDDIAMIRVT
                     ADQPGRLNCRISIDRPEKGKSQVRGRDRDREGNRDPELQLSGQLDNGVDGKGMSYLAR
                     IRTRLTGGTCTAKGNTLVVKKATEMIVFVSAATDWKGSDHKEKTKQILADAMQKPYEQ
                     QVQEHIKAYRLLFDRMDLQLGTDTSLAALPTDQRLLRFHQNPAADNGLAVLFFQFGRY
                     LSISSTRVGLLPPNLQGLWANQVHTPWDGDYHLDINVQMNHWPVEVTNLSELNLPLTD
                     LVAGMVPQGEKTAKAYYNAGGWVAHVITNPWGWTEPGESASWGVTKVGAGWLCDNLWQ
                     HYDFTGDTGYLRRIYPILKGAAQFYNDMLIEDPRHGWLVTAPSSSPENWFTLPNGHNA
                     SICMGPAIDNQIIRQLFGNVITAAQILKKDEAFRSTLAERLQHIPPAGIVAPDGRLQE
                     WLEDYKETDVHHRHVSHLYGLYPAALITPDSTPLLAEACKKTLEARGDDGPSWSIAYK
                     MIWWARLYDGNRAYRILTEIMTPTLATNINYGAGGGIYPNLLSAGPPFQIDGNFGSTA
                     AIAEMLVQSHDGCIHLLPALPDAWKAEGNVRGLKARGNFTVDITWKDGKVVQYHISSP
                     HPGKLSPLQPHKVKVKVNGVVTEIIAD"
BASE COUNT         1284 a         1704 c         1758 g         1453 t
ORIGIN      
        1 gaagccttct tccagaacac gccgagcagg aacacacagg tgatgggcgg ggcgatatgg
       61 gcgatgatat cgtttagtcc gttgaagata ctctcatacc ggttgagcaa gggaagcagg
      121 gcgagggaca acagtaatgc catgcctgcc gagatgcgac ccatgcgcac cagctgtctg
      181 tcatctgcct ccggtcgtct gcgtcggtag atgtcatagc ttaccaatgt agagatcgag
      241 ttcaatgcgc ccgagacctg ccccatcagt cccgacagca gggccgcgac caggatgccc
      301 accagtcccg aaggaagcag ctgggtgatc atcagcgtgt aaataccctt gctgttcaca
      361 tcgccgccgg ccgttctcag cgaatgaata tccaaccggc ccgttgcggc cagcgtatag
      421 gcaaacaatc ccggcaggac gaaaatgaat acgggtaata tttttatgaa cccgcagaac
      481 agggggccga tacgggcatg ttgttcatcg cgggcgccca ggacccgctg tacgatcgtc
      541 tggtccgcgc accagtacca gatgcccagg acgggatagc ccaggaagac ggagtaccag
      601 ggcatgccgc tgctgtcgcc ggcgggacgc agcatggaca aacggttgat ctgtccgtgg
      661 gcgtggagtg tgtccgtcat cgcgtgccac ccgcccatcc gatgccaggc ggccatactg
      721 atgataacgg cgccggtgat gagcacgacg gtctgtatgg actcggtgac gaccaccgcc
      781 ttcagcccgc cgatgatggt gtagagggcg gtgatcaagg agatcaccac cacgctggta
      841 tacatgctga ccccgaacaa ggtctttagt acgatgccac cggccagcat ggaaaaggcg
      901 atatggatga tgacggcaga tacgatggag accatgacca gccagtcgcg gctcgaacgg
      961 tcgtaccgct tctccaggaa gtcgggcagg gtggtgacgc cggaagtaat atagaatggg
     1021 acaaagaaca aggccagcag gatgagggtg aatgcggcca tccattcaaa atccccgttc
     1081 agcaggccgg tgtcgaagcc gctctgcgcc aggctcacga ggtggacggt ggagatattg
     1141 gtggcgaaca gcgccagccc gatggcgggc cagcgcaggg ttttgccggc caggaaatat
     1201 tcccccgcgt tcttccggcg gtgacggatg cccacgtata accctatcgc aaggatggcg
     1261 aggatataca aaatgctgac cacgatgtcc aaggttctga tcatattgct attggtatgc
     1321 aggctgtttc tctaccagtg tatccaacag gggaaatagc tcattctgcc atggacgcgg
     1381 ggcattgctg tccacgatcc tgcccgccgc atcgatgagg atgtagtgtg gtattcccct
     1441 aacctgatag tcattgcaga ctttattatt cgtccatcct tccgcgacga gattaacgcc
     1501 ttccaaatgc agcttttcga ggccttcctt ccatgtcttt tcatcactgt ccacacagat
     1561 gtttacaaaa acaatgttct tatctttata cctttcatgc agggccggaa catgattttt
     1621 aatatcgtcg atacaggggg cgcaatcgtt gccccaaaaa tcaatgtaca ccacatgacc
     1681 tctgagatct gccagtgaca cattcccgcc tttttcattt tgaagcacaa aggcaggagc
     1741 cgggtttccc gacctcagct tctgaaggcc ggcgtagaac tgcctaagcg tatcagcata
     1801 gggacgaaaa gtaactttcg taatgaagtc ccggtaaacc gtgtcgatct cgtcgaatga
     1861 aaagaagtct ggtgcgtcca ttatcaacct cgtgacgaac caatccctga tctcccggct
     1921 tcgaaaactt ctcattccat tgtaatagtc attttgcagg tagtgaatag cgtccttgct
     1981 gtcccaggtc atgaacggag tgaacccgaa ttttatatat tccaacagga attcccggta
     2041 ccagtgactg gtcctgaatg tttcctccga ttctgcctgg tagggtcgaa gagcatgcca
     2101 ttggtcctgc cttgccagga acggcaggtc tgtgccttca tagggatgct ccaggctcag
     2161 atcatgtcct gtcaacaaaa aatgatctgc caaaaaagta gcatattgat aatagacgtc
     2221 ttctatgaat ttgttcgtat gtgtcgtccg cggccgggtt aagagcgcgg tgatttcgtc
     2281 attgtacaga tgatcgatct tattgaaatt tacagaatcc ggcaacttcc gggagtaaga
     2341 tacaatgatg tccctatacc ggggctggaa ctgttggtac aggaaaagct gtgtcttcag
     2401 ttccgtatca cgttcagcct tcggactgct gacggacagg gatggcccgg cgtctttacc
     2461 atcccagcta aggtcgatgg tgtcgcctgg ctgaataaaa ataaacaggt tgtagccgac
     2521 ggcaagggtc atctcctgtc cccccggcct tatcggtatt tctttgctga acctgccctt
     2581 tctgtcaatg gcaatcgact cttgcggatc tctcaggtag ccgtatattc tatatcccaa
     2641 ggaggtatcc ctattgttct tcacctgccc cctgatcagg gcagatgctt tccccgatga
     2701 ggatgtagtt tcctgcgccg gcaggtttat tgccaccagc aaggcggctg tcagcaaacc
     2761 atgcttcata agccatcgat taaatcgcaa ctgctgcctg gttcctgtgg ggtggcatat
     2821 acaccgcccc ggacgtcggc cggatgttga aaatattttt tcagatgagg gatatgctcc
     2881 aggaacagcg cttcatgtcc cagggaaata tgattgaaca acaccaggtg ttggtgcagt
     2941 tgtcccatgt ctccgacatg cggcaccacg ggaataccga agagccggct gagcaggctg
     3001 acggtgatga actcacttac tccccccacc cgtacggcat ccacctggat gaaaccggct
     3061 gcgccggcct gcaggtaatt tttaaagacg atcctgttgg gtacgtgttc gccgatggcc
     3121 agtcttgtgg gggccagcgc ttccgccagt gtcttatgcg ccagtacatc gtccggatgg
     3181 gtaggctctt ctatccagaa agggtccatg ggttgcagat gtttaccgat gcgtaacgcc
     3241 tgtggcaggg tccactgctg gttggcatcc accataatgc ggatgtccgc tcctgctgct
     3301 tcccgcacga tgttggcgcg ccggatatcc cgggtggggt ctgccgaccc gaccttcagc
     3361 ttcatggccg tgaacccttc ggctactgcc cggatggtgt tctcccggac ctgctcgtcg
     3421 gagtagttga accagccgat ggaggtgtcg tatcccgggt atcctttctc caggatcttc
     3481 tcccgcgaaa ccttcccggg taccttctcc cgcaacagct gcagggccct gggggaggga
     3541 agcacgtctt ccaggtagga cagatccagc gtattcagca gctcttccgg cgaaaggtcg
     3601 atgagcaatt tccataaagg caggccccgt ttttttgccc agagatcgaa acaggcattg
     3661 gtgacggaag ccagcccgag atgcaccacg cctttgtgcg gacccagcca gcggaactgt
     3721 tgttcgttgg aaagggcatt gaactccctg ccgaagccgg ccatcaactc ctcaagatct
     3781 ctttccagca agcgccgggc atagaagcgg gccgcttcgc agaccatatc gttcccttcg
     3841 ccgagggtaa aggccaagcc ggtgccggtg tgaccgctat cgtcctgcaa ttgggtcacg
     3901 gcataggaat acacggggtc ccggtgaatg gcgtcgctgc ccgcgccttt ggccagggga
     3961 aaacgaaggt cttttaccag tatacttttg atcatagact attttcgata tgcgtggatc
     4021 catcggcgat gatcttgtgt ggatccttcg gcgatgatct ggtttggttc aatcggcgat
     4081 gatctcggtt acgacaccat tcaccttcac ttttactttg tgtggctgga gggggcttag
     4141 cttgccggga tggggggagc tgatgtgata ctgaacgacc ttaccgtcct tccacgtaat
     4201 atctacggtg aaattgcccc gcgccttcaa tcccctcacg ttcccttcgg ccttccaggc
     4261 gtccggcagg gcgggtagca ggtggatgca tccgtcgtga ctttggacca gcatctctgc
     4321 gatggccgcc gtcgagccga agttgccatc tatctggaaa ggcgggccgg ccgacaacag
     4381 gttggggtaa atgcctccac cggcgccgta gttgatgttt gtcgccaggg taggtgtcat
     4441 gatctccgtc agtatccggt aggccctgtt gccatcgtag agcctggccc accagatcat
     4501 tttataggcg atgctccagc tcgggccgtc gtcgccgcgg gcctcgagtg tttttttaca
     4561 ggcttccgcc aggaggggtg tggaatcggg ggtgatcagg gcggcgggat acagcccata
     4621 gagatgcgac acgtggcggt ggtgcacgtc ggtctcctta taatcttcca gccattcctg
     4681 caaccggcca tccggcgcca cgatgccggc cggggggata tgttgtagtc tttcggccag
     4741 cgtcgaacga aaggcctcgt ctttctttag gatttgggcg gccgtgatca cgttgccgaa
     4801 cagttgccgg atgatctggt tgtcgatggc gggccccatg caaatactgg cgttatggcc
     4861 attgggcagg gtgaaccaat tttccggaga agaagagggc gccgtcacca gccagccgtg
     4921 ccgcggatct tctatcagca tgtcgttata aaattgggcg gctcctttta ggatgggata
     4981 gatgcgccgc agatagccgg tgtcgccggt gaagtcataa tgctgccaga gattgtcgca
     5041 aagccagccc gcccctacct ttgtcactcc ccaggaggca gactcgccgg gttccgtcca
     5101 gccccaggga ttggtgatga catgcgccac ccatccaccg gcattgtaat aggcttttgc
     5161 cgtcttctcc ccttgcggga ccatgccggc gaccaggtcg gtgagcggca ggttcagctc
     5221 ggaaaggttg gtcacctcca ccggccagtg attcatctgc acgttgatat ccaaatggta
     5281 gtcgccgtcc cagggcgtat gcacctggtt tgcccagagc ccctgcaaat tggggggcag
     5341 caggccaacc cgggtgctgc tgatgctgag gtagcggccg aactggaaga agagtacggc
     5401 caggccgttg tccgccgccg ggttctgatg gaagcggagg aggcgttgat cggttgggag
     5461 ggcggcaagg gaggtatcag tcccgagttg caggtccatt ctgtcgaaca ggagtcgata
     5521 ggcttttatg tgctcctgca cctgctgctc atatggcttt tgcatagcgt ccgccaggat
     5581 ctgttttgtc ttctctttat ggtcgctgcc tttccagtct gtcgcggccg atacgaatac
     5641 gatcatttcc gttgcctttt tcacgaccag cgtattgccc ttcgcggtgc aggttccgcc
     5701 ggtgagcctt gttcttatcc ttgccaggta ggacatgccc ttcccatcga cgccgttgtc
     5761 gagctgaccg gataactgta gttccggatc cctattccct tccctatccc tatccctacc
     5821 cctgacctgg gacttgcctt tctcgggtct gtcgatcgag atgcggcagt tcagcctgcc
     5881 cggctgatcc gcggtcaccc ggatcatggc gatgtcgtcg ccaaaactgg tgaggtattc
     5941 ccttttgtac gtaacgccag ctactttgta tgtacagcgc gccatggcgt catgtagtat
     6001 cagttcgcgg gaatagtggt cgaatgccgg gctgtcctta gcacggactc cgtctgatgc
     6061 ctcataatcg aattgaaggt ggagattgcc gagcatttga aaacaaccat agcgtgtacc
     6121 gccggatccc gggcctgtac aaatgaatga tttgtcgacc agtgcctggg ccgagtcgtt
     6181 cttgccttcc aggaggtat
//
LOCUS       DATGFG010000641         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_236283, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000641 DATGFG010000000
VERSION     DATGFG010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236283"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VKQ52_12020"
     CDS             complement(<1..227)
                     /locus_tag="VKQ52_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ87967.1"
                     /translation="MQRYLFLPIFLLLAQATAHAQPADGILTLPAALRIAVTNYPQIK
                     AAQHMANASALELKAAKQDGLPDFTAGIQLA"
     gene            complement(394..762)
                     /locus_tag="VKQ52_12025"
     CDS             complement(394..762)
                     /locus_tag="VKQ52_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLZ87968.1"
                     /translation="MITAYHLYTGADGDSHVVRGQIPDATLVKAESIQFKETAAHSTF
                     DWHDAPNTQYVITLSGTLEFTTRGGETFTLRPGDILVAMDTTGSGHKWRLVDDQPWRR
                     AYIIFADGADPNFIPDNNKA"
     gene            complement(759..2261)
                     /gene="mqo"
                     /locus_tag="VKQ52_12030"
     CDS             complement(759..2261)
                     /gene="mqo"
                     /locus_tag="VKQ52_12030"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF003603.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HLZ87969.1"
                     /translation="MSTTAKTGSRPGIALVGAGIMSATLAVMLKELDPSLNITIYEIL
                     DSPAQESSNAWNNAGTGHAALCELNYTPENGDGSIDIHKALEVNTEFDLSRQFWSYLV
                     TKGAIKDPRTFIHSVPHHSFVRGGDNVAYLQRRFTALSAHPLYQGMEYTTDRQQLKEW
                     FPLVMEGRDTGELVAATRMITGSDVDYGALTRQLLDILKGMDGFDIQYSTRVQDLHRT
                     DSYWDLKLRDERTGHHRHTLASFIFLGAGGGSLPLLQKSKIAEGRGYAGFPVSGVWLR
                     CDNPAVATRHHAKVYGKAAVGSPPMSVPHLDTRHIDGQVSLLFGPYAGFSTKFLKHGS
                     YLDLFGSIDPDNILPLLAVGRDNFSLEKYLVGQVFESEKDRFAALREFFPLAKEEDWR
                     VEVAGQRVQIIKKDPEHGGVLQFGTELVGAADHSLVAMLGASPGASTAVSIMIHVIER
                     CFAPQLSNGWGEKLKPMIPSHGQSLIADPTLAHRLRATTAEALHLQNVQP"
     gene            2509..3015
                     /locus_tag="VKQ52_12035"
     CDS             2509..3015
                     /locus_tag="VKQ52_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87970.1"
                     /translation="MKKNTLLVLLLLSFFFSRAQSHGYGMIRAVDFNYGHTDPALGGM
                     FSFGVKGKVVGAGVGTAILHIPGSSSPYFPVFGELNFYTDLRQVRPILNLQAGYGIYS
                     QDLGFGVTEKGGLYLGPNLGLLFPMAGRTAFLFSVGYVRTTLSVSAGGESASASTDGW
                     SVSAGLKF"
     gene            complement(3074..>3982)
                     /locus_tag="VKQ52_12040"
     CDS             complement(3074..>3982)
                     /locus_tag="VKQ52_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ87971.1"
                     /translation="NPPPLSSFGPLKQIDAGVLKVSYAEAGPANGPVVILLHGWPYDI
                     HSYADVAPLLAAKGYRVIIPSLRGYGATSFLSAETIRNGQQSAFAVDTIALMDALHIG
                     KAIVAGFDWGARTADIMAVLWPDRCKALVSVSGYLIVNLIANAKPLPPKAELGWWYQY
                     YFATERGRLGYDANRHEFNRLIWTLASPKWNFDDATFERSAAAFNNPDHVAIVIHNYR
                     WRLSLAEGEAKYDHLEKKLSEAPVITVPTITIGSDFDGAAVDGKAYAAKFSGKYTHKI
                     LPGIGHNVPQEAPKAFADAIIEVDGY"
BASE COUNT          740 a          974 c         1366 g          902 t
ORIGIN      
        1 taggccagct ggataccggc ggtgaagtcg ggaagcccgt cctgttttgc tgctttcagt
       61 tcgagggcgg aggcgttggc catatgttgt gccgctttta tctgcgggta gttggtaacc
      121 gcgatgcgga gagccgccgg caatgtgagg atgccgtccg cgggctgagc atgggccgtt
      181 gcctgtgcca ggaggaggaa gatggggagg aagagataac gttgcatggt cggctggttt
      241 ttgttgtcgg tgtaaaacta gcgccgcgcg gaggggatgc ccgggacgaa tcgccgaatg
      301 ggcaaaagct gtcgatggtt gctggaggaa agctggaggg aaagggccgg tttcccggaa
      361 ataggaaagc cgcctggcgg cggctttcaa cagttatgcc ttgttgttgt ccgggatgaa
      421 gtttgggtct gcgccgtcag cgaagatgat ataggctcgt cgccagggtt ggtcgtcgac
      481 cagccgccat ttatggccgg agccggtggt gtccatcgcg acgaggatgt caccgggccg
      541 gagggtgaag gtctcgccac cacgggtagt aaattcgagt gtacccgaaa gggtaatgac
      601 atattgcgtg ttgggtgcgt cgtgccagtc gaaggtggaa tgcgcggcgg tttctttgaa
      661 ctggatggac tcggctttga cgagcgtcgc atccgggatc tgaccacgaa ccacgtggga
      721 gtcgccgtcc gccccggtat aaagatgata cgcggtgatc atggttgtac gttttgcagg
      781 tgtaaggctt cggcggtggt ggcgcggagg cggtgtgcca gtgtggggtc ggcgatgagc
      841 gactggccgt gcgaggggat catcggtttt agtttttcgc cccagccatt cgacaattgg
      901 ggggcgaaac agcgttcgat gacatggatc atgatcgata cggccgtgga tgcgccgggg
      961 cttgcaccga gcatcgcaac gaggctgtga tcggcggcgc cgacgagttc ggtgccgaac
     1021 tgcaggacgc cgccgtgttc cgggtccttc ttgatgatct gtacccgctg tccggccact
     1081 tccacacgcc agtcctcttc ctttgcgaga gggaagaatt cgcggagggc ggcgaagcgg
     1141 tctttttcac tttcgaagac ctggccgacc aggtattttt cgagtgagaa gttatcgcgg
     1201 ccgacggcca gcaggggcag gatattatcc ggatcgatgg agccgaagag atcgagatag
     1261 gagccgtgtt tgaggaattt cgtcgagaag cccgcataag ggccgaagag cagggagacc
     1321 tggccgtcaa tgtggcgggt atcgagatgg ggtacagaca tgggcgggga gccaaccgcg
     1381 gccttcccat agacctttgc gtgatgacgg gtggcgacgg cagggttgtc gcagcgaagc
     1441 cagacgccgc ttaccgggaa gccggcatag ccgcggcctt cggcgatctt tgatttttgc
     1501 aggagcggga gtgatccgcc gccggcgccg aggaagatga aggaggcgag ggtgtggcgg
     1561 tgatggccgg tgcgttcatc gcgaagcttt agatcccagt agctgtcggt acgatgaagg
     1621 tcctggacgc gggtggaata ttggatatcg aagccatcca ttcctttcag gatgtcgagc
     1681 agttgccggg tgagggcgcc atagtcgacg tcgctgccgg tgatcatgcg ggtagcggct
     1741 accagttcgc cggtgtcgcg gccttccatc accagcggga accattcctt tagttgttgt
     1801 ctgtcggtag tgtattccat cccctgatag agcggatggg cggagagggc ggtaaagcgt
     1861 ctctgcagat aggcgacgtt gtcgccgccg cgaacgaagc tgtggtgggg cacggaatgg
     1921 ataaaggtgc gtgggtcttt gatcgcgcct tttgtcacca gataggacca gaactgccgg
     1981 gagaggtcga attcggtgtt tacctcgagg gccttatgga tatcgatgct tccgtcgccg
     2041 ttctcggggg tgtaattgag ttcgcagagg gcggcgtggc cggtgccggc gttgttccag
     2101 gcgttggagc tttcctgtgc ggggctgtcg aggatctcgt agatggtgat gttgaggctg
     2161 gggtcgagtt ctttcagcat caccgccagt gtagcgctca tgatgcccgc gccgacgagg
     2221 gcgatgcctg ggcggctgcc ggttttggca gttgtggaca tgatataggt ttttgtatta
     2281 gtgccttttt agcggcataa agataaggat gttcttcttt tatgacatga atgcgctgat
     2341 catgatgcgg gtccggacgg cgatgtcgaa tccggtgaag gcgttgcgtg gcgaataagc
     2401 cggggcgccg gcggggaatc gggaagagta tgaaatgcgg cgccgatata ttatattggc
     2461 gtagtcctgc gttgttcctg gacctatcgt taacctataa accgcgttat gaaaaaaaac
     2521 acattgcttg tccttctcct cctttccttt ttcttctccc gggcccaaag ccatggttat
     2581 ggcatgatca gggccgtcga tttcaactat ggccataccg atcccgccct tgggggcatg
     2641 ttctcttttg gcgtcaaggg caaggtcgtc ggcgccggcg ttgggacggc catcctgcat
     2701 attcccggca gcagcagtcc ctattttccc gtgttcgggg agttgaactt ttacaccgat
     2761 ctccggcagg tgaggcccat cctcaacctg caggccggct atgggatcta ttcccaggac
     2821 ctcggttttg gagtgacgga aaagggcggg ttgtatcttg ggcccaacct cgggctattg
     2881 ttcccgatgg cagggcggac cgccttttta ttcagcgtgg ggtatgtacg cacgacctta
     2941 agcgtcagcg ccggcgggga atcggcgtca gcctcgacgg atgggtggtc ggtcagcgcg
     3001 gggttgaagt tttgaccgtg cgggcgtccg gcgacgggca gccattgctt cggcttagta
     3061 accgtcgacc tcgttagtaa ccgtcgacct cgatgatggc gtcggcgaag gcctttgggg
     3121 cttcctgggg aacattatga ccgatgcccg ggaggatctt gtgtgtatac ttacctgaaa
     3181 atttggcggc ataggccttc ccatccacgg ccgcgccatc gaaatcgctg ccgatggtga
     3241 tggtgggtac ggtgatgacg ggtgcttcac tcaacttctt ttcgagatgg tcatattttg
     3301 cttcgccctc tgccaggctg aggcgccagc gatagttgtg gatcacgatg gcgacatgat
     3361 cgggattatt gaaagccgca gcgctgcgtt cgaaggtagc atcgtcgaag ttccattttg
     3421 gcgaggcgag ggtccagatg agccggttaa actcgtggcg gttggcgtcg tagccgaggc
     3481 ggccgcgctc cgtggcaaaa taatactggt accaccagcc caattcggcc tttggcggca
     3541 ggggctttgc gttggcgatg agattgacga tgaggtagcc gctaacggat acgagggctt
     3601 tacagcgatc gggccagagg accgccatta tatctgcggt acgggcgccc cagtcgaagc
     3661 ccgcgacgat ggcttttccg atgtgtaagg catccatcaa cgcgatggta tctacggcga
     3721 atgccgattg ctgtccgttg cggatggttt cggcggaaag gaaactcgtg gcgccatagc
     3781 cacgcagaga agggatgatc acccggtagc cttttgccgc gagcaagggc gccacgtctg
     3841 catagctatg gatatcatag ggccagccgt gtaagaggat gactacgggc ccattggccg
     3901 ggccggcctc ggcatagctg accttcaata cgccggcatc gatctgcttc aacggaccga
     3961 aggaggaaag tggcggcggg tt
//
LOCUS       DATGFG010000642         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_47881, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000642 DATGFG010000000
VERSION     DATGFG010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47881"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1410
                     /locus_tag="VKQ52_12045"
     CDS             157..1410
                     /locus_tag="VKQ52_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ87972.1"
                     /translation="MPKKIVILTTGQPSTNPRMVKEVEALREAGETIIVFYSYWAAWA
                     LPADEELLARHPGVFRMVGGSPGKDRLSWLLTRLFYKMAFRLGGRVPFFRQYALARTT
                     RSLEKAALRERADLFIAHNLGSLPAAVKAARKWGAAVGFDAEDYHRGEFERPEGPRYR
                     YTAYVEETFMPACDYLTAASPLIAEAYEKILGGKRLTVVNNVFSRDYLQPERRSTGRG
                     LRLFWFSQTVGPDRGLELIVRAIALLPEYEIRFDIAGACSDAYKALLTGFTNGKDYLE
                     FIPPVSPDRLFPLSAGYDIGMAAEVPHSENRDRCLTNKLFTYLLAGNGILASSTSAQR
                     RFMEENPGIGYVYDAADARDLAACLSRLYTDRALLEDCRRRSRALGADRYNWETEKLV
                     FLEQIAPSLKKNEKDPDHRPAVSAQ"
     gene            1367..>2599
                     /locus_tag="VKQ52_12050"
     CDS             1367..>2599
                     /locus_tag="VKQ52_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ87973.1"
                     /translation="MKKILIIAPQFPPNNQAAVHRTRLFAQHLPAFGWEPVILTIREK
                     YYEEKPDWNLVKLLPPALRVERTNAFPITRPRLIGDLGLRAFFHLHRKARRMLKEGSF
                     DFVYLPIPPFYTALIGRWLHAGTGIRYGIDYIDPWVHRFPGSEKKFSRHWWSSQLAKL
                     LEPIAVKRASLITGVAEGYYQGVLERNPRLAERVVFGAMPYGGEEKDHDKVKELGLQP
                     YLFSRKPGKLQLVYAGAMLPKAYGPLEAMLQTIRDNPGGFADVEFHFIGTGKTTNDAN
                     GHTIKPLAEKYGLWEKTIFEYPARIPYLDVLIHLDAADGVFVLGSTEPHYTPSKVFQG
                     ILSHKPVMALLHEASSARKVLLESGAGSVLAFNGDDYRQRIEGGFIPAFEQYRAFLRE
                     YDFTKVNRELFSEYSAYKV"
BASE COUNT          523 a          715 c          809 g          552 t
ORIGIN      
        1 cgctgatgcg ggaggtgctc aatttcatgt atgattttgg gttcgtggtc tatgatatct
       61 gttcggtctc gacacgcaga cctcttgata aagccttgtg gcagacagac ctcctgtttg
      121 taagggaaga cagtatcttc cggtcagaca agcgatatgc ctaagaagat cgtcattctt
      181 acaaccggcc agccctctac caatccccgg atggtgaaag aagtggaggc attacgggaa
      241 gccggtgaga ctatcatcgt attttatagt tactgggccg cctgggcatt accggcggat
      301 gaggaattgc tggcccggca tcccggtgtg ttccggatgg taggcggcag tcccggaaag
      361 gatcggctct cctggctgct gacgcgattg ttctacaaga tggcgttccg gctgggaggc
      421 cgggtaccct tctttcggca atatgccctg gcccggacga cccgatcgct ggagaaggcg
      481 gcgctccggg aaagggccga tctctttatc gcgcataatc ttggcagtct tcccgcggcc
      541 gtaaaggcgg cgcggaaatg gggggcggcc gttggcttcg atgccgagga ttatcaccgc
      601 ggggaattcg aacggcccga ggggccgcga taccggtaca ccgcctatgt ggaggagacc
      661 tttatgccgg cctgcgatta tctcaccgcg gcttcgccgc tgatcgcgga ggcctatgag
      721 aagatcctgg gcggcaagcg gctgacggtg gtgaataatg tattcagccg ggactacctg
      781 cagccggagc ggcgatccac gggccggggg ctacggcttt tctggttctc ccagacggtg
      841 ggtcccgacc ggggtctgga gctgatcgtc cgcgcgatcg ccctgttgcc ggaatacgag
      901 atccggttcg acatcgcggg ggcctgctcc gatgcataca aggcgcttct caccgggttt
      961 actaacggga aggactatct cgaatttatc ccgccggtgt ccccggaccg gctgttcccc
     1021 ctgtcggcgg gttacgatat cggaatggcg gcggaggtcc cgcacagcga gaaccgcgat
     1081 cgttgtctta ccaataaact gtttacctat cttcttgccg ggaatgggat cctggcttcc
     1141 agtaccagcg cccagcgccg cttcatggag gagaacccgg ggataggata tgtctatgat
     1201 gccgccgatg cccgcgacct ggctgcctgt ctgtcgcggc tgtataccga ccgggcgttg
     1261 ctggaggatt gccgccggcg ttcccgggcg cttggcgccg accggtataa ctgggagacc
     1321 gaaaagctcg tctttctcga acagatagcc ccctccctga agaaaaatga aaaagatcct
     1381 gatcatcgcc ccgcagtttc cgcccaataa ccaggcggcc gttcatcgta cccggctttt
     1441 tgcccagcac ctgcctgcct ttggctggga accggtgatc ctgacgatca gggaaaaata
     1501 ttacgaagag aaaccggatt ggaacctggt gaagctgctc cctcctgccc tgcgggtgga
     1561 gagaacgaat gcctttccga tcacgcggcc gcggctgatc ggcgacctcg ggttgagggc
     1621 ttttttccat ttgcaccgca aggcgcggcg gatgctgaaa gaaggatcat tcgactttgt
     1681 ctacctgccc attccgcctt tttatacggc gctgatcggc agatggctgc atgccggaac
     1741 ggggatcagg tacggcatcg actatatcga cccctgggtg caccgtttcc cggggtccga
     1801 aaaaaaattc tcccgtcact ggtggagcag tcaattggcg aagctgctcg agcccatcgc
     1861 ggtaaagcgg gcctccctga tcacgggcgt ggcggaagga tattaccagg gagtgctgga
     1921 aaggaatccc cggctggcgg agcgggtcgt ttttggcgcc atgccctatg gcggggaaga
     1981 gaaggatcat gacaaggtga aagagctggg actgcaaccc tatctttttt cccggaaacc
     2041 gggcaagctt cagctcgtgt atgcgggggc catgctgcca aaggcctatg gaccgctgga
     2101 agcgatgctg cagacgatac gggataatcc ggggggcttt gccgatgtgg aatttcattt
     2161 tatcggcacc ggtaagacga ccaatgacgc caacgggcat acgataaagc cgctcgctga
     2221 aaagtacggg ctttgggaga agaccatttt cgaatacccg gcacggatac cttacctcga
     2281 tgtgctgatc cacctcgatg cggccgatgg ggtctttgtg ctgggcagca cggagccgca
     2341 ttacacgccg tccaaggtct tccaggggat cttgtcgcat aagccggtga tggccctgct
     2401 gcatgaggcg agcagcgcca ggaaggtgct tctcgagtcg ggcgccggat cggtcctggc
     2461 gttcaacggc gacgactaca ggcaacggat cgaaggcgga ttcatcccgg ccttcgaaca
     2521 ataccgcgca ttcctccggg agtatgactt tacaaaagtg aacagggaac ttttctcgga
     2581 atatagtgct tacaaggtg
//
LOCUS       DATGFG010000643         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_163977, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000643 DATGFG010000000
VERSION     DATGFG010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163977"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VKQ52_12055"
     CDS             <1..464
                     /locus_tag="VKQ52_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87974.1"
                     /translation="DGSLFFMRPVADTGKFHLYRSAYQNGKYQTPAEVSFSAPDNFSD
                     VDPAVAADESFLVFSSKRPPATQMELFIVFRKGGVWGVPQQLGAEVNRGTYSIEARLS
                     PDGRRLYFSSVYLARPAATGDVLSRRRELERSEWETGLLNIWSVPMDKWVK"
     gene            583..1497
                     /locus_tag="VKQ52_12060"
     CDS             583..1497
                     /locus_tag="VKQ52_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ87975.1"
                     /translation="MQPTETLQQFYARVRKTEAEGIPLNNAGSGHFNVFPRDTCYRAT
                     PYGRRDFYKVSFILGTGRLHYADRLIGMDRPSLLFSNPMIPYSWEAVSTEQKGWYCLF
                     TEAFVHPVERKETLQDSPLFQTNGMPVFFLDKDQQEEVGDIFRKMMAEMASDYVHKFD
                     VLRNYLHLLIHQAMKLRPVSLVERPAGAAARITQQFLELLERQFPVDTTETELQLKTA
                     NDFAEKLSVHVNHLNRALKEVTGKTTTEHISARVIREATALLRHSDWNIAQIAYGLGF
                     EYPAYFNIFFKKHTGITPLQARNAVMPG"
     gene            1671..>2371
                     /locus_tag="VKQ52_12065"
     CDS             1671..>2371
                     /locus_tag="VKQ52_12065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ87976.1"
                     /translation="MSALFRSTKRINERYQPFATLLSLALLPLSGFATDIYVPSLPYM
                     ADALHVSSLQIQVTITLFLVSYGVSQLFIGSVLDSFGRYRIGLASLVIFSLASLVIGL
                     THSIHLIYLMRIIQGVTVGAAVAGKRAYFVDLFTGGKLKHYLSLFSIIWSTGPIVAPF
                     VGGWMQTAFGWEMNFYLLALVGAVFAVLEFLFSGETLPQPTAFRLRRVVGVYAGMLKT
                     GEFVLGILLLGLAYS"
BASE COUNT          429 a          525 c          805 g          612 t
ORIGIN      
        1 cggatgggag tctttttttt atgcggccgg ttgccgacac gggtaaattt catctgtatc
       61 gcagcgccta tcaaaatgga aagtatcaga cgccggcgga ggtgtcgttc agcgcgccgg
      121 ataatttcag tgatgtcgat cccgctgtgg cggcggatga gtctttcctg gtgtttagca
      181 gcaagcggcc gccggccacg cagatggagc tttttatcgt tttccgcaag ggtggggtat
      241 ggggtgtgcc gcaacagctt ggtgcggagg tgaaccgggg aacctatagt atcgaggcga
      301 ggctgagccc ggatggccgg cggttgtatt tttcgagtgt ctacctggcc cggcccgcgg
      361 ctaccggcga tgtccttagc aggcggcggg aactggaacg gagcgagtgg gagacggggc
      421 ttttgaatat ctggtcggtg ccgatggaca aatgggtgaa gtgatgggcg aggcggcggg
      481 taaggtgatg ggcagggaca gatggggatg attggggatg atcggggatg gatggggatc
      541 ggcggggttg gtggggttgc ttgaatttta taattttgtc ttatgcaacc gacggagacg
      601 ctacagcaat tctatgcccg ggtgcgcaag acggaggcgg agggtattcc cctgaacaat
      661 gcgggatcgg ggcatttcaa tgtgtttccc cgggatacct gttaccgggc tacgccgtat
      721 ggccgccggg atttttacaa ggtgagtttt atactgggca cggggcggtt gcattatgcc
      781 gaccgattga tcgggatgga ccggccctcg ctgctttttt ccaacccgat gatcccttat
      841 tcctgggagg cggtgtcgac cgaacagaag gggtggtatt gtctttttac cgaggctttt
      901 gtacatcctg tcgagagaaa ggagacgttg caggattcgc cgcttttcca gacgaatggg
      961 atgccggtgt tcttccttga caaggatcag caggaggagg tgggggatat cttccggaag
     1021 atgatggcgg agatggcgtc ggattatgtg cataaattcg atgtgttgcg taattacctg
     1081 catctgctga tccaccaggc gatgaagttg cggccggttt cgcttgtcga gcggccggcg
     1141 ggggcggcgg caaggatcac gcagcagttc ctggagctgc tggagcggca attcccggtg
     1201 gacaccacgg aaacggagct gcagctgaag acggccaatg attttgcgga gaaattgtcc
     1261 gttcatgtca atcacctgaa ccgggcgctg aaggaggtga cgggtaagac gaccaccgag
     1321 catatttcgg cgcgggtgat ccgggaagcg accgctttgt tgcggcattc agactggaac
     1381 atcgcgcaga tagcgtatgg gcttgggttc gaatatcctg cctatttcaa tatcttcttt
     1441 aagaagcata cggggatcac ccccctgcaa gcgcgaaatg cggtgatgcc gggctgatgc
     1501 gggccgttgg acgccgatgt gggccgttgg gagccgatgt gggccgatgg gccctgatat
     1561 gggcgttgtg ggcggatctg ccattgtttg aattttataa tgatcggtgt ggatactgta
     1621 atgcggggat gggatggccg gggtagcttt gcaggcataa atcgacggtt atgtctgcat
     1681 tgtttagatc gactaagaga atcaacgaac gttatcagcc ttttgcgact cttctttcac
     1741 ttgccctgct tccgctttcg ggttttgcga cggatattta tgtgccttcc ctgccgtata
     1801 tggcggatgc gttgcatgtg agcagtttac aaatacaggt taccattacg cttttccttg
     1861 tcagttatgg ggttagccaa ttgtttatcg ggagtgtgct ggacagtttt ggccggtacc
     1921 ggatcgggct ggcttctctc gtgatcttct cgctggcgag tcttgtcatc gggttgacgc
     1981 actcgatcca tcttatctac ctgatgcgga tcatccaggg ggtgacggtg ggtgcggcgg
     2041 tcgcgggtaa gcgggcctat tttgtggacc tgttcaccgg gggcaagctc aagcattatc
     2101 tgagtctttt ttccatcatc tggtcgacgg ggccgatcgt ggcgcctttt gtcggcggct
     2161 ggatgcaaac ggcgtttggc tgggagatga atttttattt gctggcgctg gtgggcgcgg
     2221 tgtttgccgt cctggaattc ctgttcagcg gtgagacgtt accgcagccg acggccttcc
     2281 ggttgcgccg ggtggtgggc gtgtatgcgg ggatgctgaa gaccggtgag tttgttctcg
     2341 ggatcctgct gcttgggctt gcttattcca t
//
LOCUS       DATGFG010000644         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1600978, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000644 DATGFG010000000
VERSION     DATGFG010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600978"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VKQ52_12070"
     CDS             complement(<1..1195)
                     /locus_tag="VKQ52_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87977.1"
                     /translation="MKHTFIVLLLGISCLHADCQFKSLLNKVKDKVTSKITGKSADST
                     SAKPDAGSPATGGQGAGSGSASSGGAASQGVNGGGNAAAAGNGAATGNGGVAGSTGGA
                     GNGVAGGNSGAAGGPGAPQPPVFPTTGKMVKVTTATFGIDTDEDWNNLLRNPMVQDLI
                     SRLRQRGVTGTDKEVLIQAMQNQQAYSDIMADMQAKYGSAAAFKPTPAFVFAVFLSSY
                     DYIMTPEYIRAEVGKTDAMGGQGGLAGSMANAFAPGITTIIDIAARKSYTVANFLNIL
                     PAAIVEDMSSFQDAFGMVAYLKKYMHQPNIKMQALGQRSFHGYNANVVGVEIPVTPVT
                     DEDGKQSDALLFLHGLLSGNSNDMVRKHYDPSYKLFVETYYSHDLDGKIPAPITGDKN
                     ILHIDG"
     gene            1467..>2489
                     /locus_tag="VKQ52_12075"
     CDS             1467..>2489
                     /locus_tag="VKQ52_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HLZ87978.1"
                     /translation="MDAQLFDKLVTIRRHLHQNPELSFTEFKTATLVESQLGDLDIPT
                     ERIALTGVIGTLKKSDGPVIVLRADMDALPITEESGVAFPSQTEGIMHACGHDIHTTM
                     LLGAAHLLKKAAFSGTIKFVFQPAEEGPMRSPQPGKSGGQLIMESGKLDDAHAILGLH
                     VHPLLPVGVLSYKNGESLANVGNFAIRINGKGGHPGLMKAVIDPVAIAARLITAAHAL
                     VGPQPDPPTAVLAITHVATEAKPSFNVIPSHLLLQGSLRAVRIADYNDIVSRLRTLLH
                     QLETEFHCTIDLEFSAYYPSLLNDPALHEQLSPVYANIFGASNVTPGQDYLVGEDFSF
                     YSRAMPG"
BASE COUNT          567 a          856 c          553 g          513 t
ORIGIN      
        1 acccgtcgat gtgcaggata ttcttatccc ccgtgatggg cgcggggatc ttcccatcca
       61 ggtcatggga gtaataggtc tctacaaaaa gcttatagga aggatcataa tgttttctta
      121 ccatgtcgtt gctgttgccg ctaagcaggc cgtgcaggaa caggagcgca tccgactgct
      181 tcccatcctc atcggtcacc ggcgtcacgg ggatctccac ccctacgaca ttcgcgttat
      241 acccgtgaaa actccgctgc cccagggcct gcattttgat attgggttgg tgcatgtatt
      301 tcttgagata ggccaccatc ccgaacgcat cctggaaact gctcatatcc tctacgatcg
      361 ccgccggcag gatattcagg aaattggcga cggtgtacga tttcctggcg gcgatatcga
      421 tgatcgtcgt gatgccgggc gcaaacgcat tggccatcga ccccgcaaga ccgccctgtc
      481 cacccatggc atccgtctta cccacctccg cccggatata ctccggcgtc atgatataat
      541 cataactgct taggaacacc gcaaacacaa acgccggggt cggcttgaac gcggccgcac
      601 tcccgtattt tgcctgcatg tccgccatga tgtcggaata agcctgctgg ttctgcatcg
      661 cctggatgag aacctcctta tccgtacccg ttaccccccg ctgccgcagg cggctgataa
      721 gatcctgcac catcgggttc ctgagcaggt tgttccaatc ctcatccgta tcgatgccaa
      781 aggtcgccgt ggtcaccttc accatctttc ccgtcgtcgg gaacacaggt ggctgcggtg
      841 caccgggccc gcccgccgcg ccgctattgc ccccagcgac accgttccca gcgccaccgg
      901 tactccccgc gaccccgcca ttccctgtgg cagcaccgtt tcccgccgca gcggcattcc
      961 ccccgccatt cactccctgc gaagccgcac ctcccgaact tgcactcccc gaacccgcac
     1021 cctgcccccc ggtcgcagga ctccccgcat ccggcttggc cgatgtactg tcggcgctct
     1081 tccccgtgat cttactcgta accttatctt ttaccttgtt caggagactc ttgaactgac
     1141 agtcggcatg taggcaggaa atccccagca gcagcacaat gaaggtgtgt ttcatcagcg
     1201 caaaatacgc caaaaaacca tttcgatgac acgggactaa ctgttaagtt tgtgtgcttt
     1261 tacgtccgcc cccacattac ccaattaccc cgattggcca aatacaaaaa aaggactaaa
     1321 taaaaaagga ggcgtataga aatacacccc cgtctaacga ttgcttgcca tatgaaaatt
     1381 gtaaaaaacc aaaccttgac ggcacaatat taaacaagaa atataagtct accaaattag
     1441 tagactaaaa ttttgtaaat tgtcccatgg acgcccaatt attcgacaag ctcgtcacca
     1501 tccgtcgcca cctccaccaa aacccggagc tgagttttac cgagttcaaa accgcgacac
     1561 tggtcgaatc acaactcggc gatctggata tcccaaccga acgcatcgcc ctcaccggcg
     1621 ttattggaac gctgaaaaag agcgacggac cggttatcgt tctccgcgcc gatatggacg
     1681 ccctccccat aaccgaagaa tccggtgtcg ccttcccctc acaaaccgaa ggcatcatgc
     1741 acgcctgcgg tcatgatatc cataccacca tgctgctcgg tgcagcccac ctgctgaaaa
     1801 aagcggcttt cagcggtacc atcaaattcg tcttccagcc cgctgaagaa ggtcccatgc
     1861 gcagccccca acccgggaaa tccggtggtc agttgatcat ggaaagcgga aagctcgacg
     1921 acgcccacgc catcctcggc ctgcatgttc atcccttatt acccgtcggc gtcctctcct
     1981 ataagaatgg cgaatccctc gccaatgtcg gcaacttcgc catccgcatc aacggcaaag
     2041 gcggccaccc cggactgatg aaagccgtca tcgaccccgt cgccatcgcc gcccgcctca
     2101 tcaccgccgc ccacgccctg gtcggtcccc aacccgatcc cccaaccgcc gttctggcga
     2161 tcacccatgt cgcaaccgaa gcaaaaccca gcttcaacgt catcccctct cacctcctgc
     2221 tacagggctc gctaagggcc gtccgcatcg ccgactacaa tgacatcgta agccgtctaa
     2281 gaacgctgct ccaccaactg gaaaccgaat tccactgcac catcgacctc gaattcagcg
     2341 cctactatcc cagcctgctg aacgatcccg ccctccacga acaactgtca cccgtctacg
     2401 cgaacatctt cggcgcttcc aacgtaacgc ccggccagga ctacctggtc ggcgaagact
     2461 tctccttcta ctcccgcgcc atgccgggc
//
LOCUS       DATGFG010000645         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_945528, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000645 DATGFG010000000
VERSION     DATGFG010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945528"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VKQ52_12080"
     CDS             complement(<1..376)
                     /locus_tag="VKQ52_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87979.1"
                     /translation="MLQNYLKVAWRNLFRNKLHTGINIGGLIIGFTIGIATLLLTYQQ
                     FQWDSQHVSGKRLYEAYQVFHDPAGEHFENAFPLGQGPGYKAEAPAIERMTRIGDGGN
                     HIEYKGRDITIPVTTVDADFLSM"
     gene            612..2993
                     /locus_tag="VKQ52_12085"
     CDS             612..2993
                     /locus_tag="VKQ52_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87980.1"
                     /translation="MLKNFFVIAWRNVRKNRAYASINVLGLSLGIACGLLIFTLVHYH
                     LSFDTFHADSRRIYRIVTEWHDEAADYSQGTPSPLGKAFRKDFDYTEKVARVIDFGAL
                     VTLQDKGETKKFSERVAYAEPEYFDIFNFPLTHGDKTTALAAPGQALLSEKMAKKFFG
                     DAAAAIGRTIRVFNQTDFQVVGILRDIPVNTDRKTEIYLSYGSLKDHDPRHASDSSWG
                     SVYSQCMTFIRLKPSVTVAQTNKALSIISKKYQEGRDAQTVVFRLQPLADIHFNPTFD
                     GIVDKKYLWALAFIGLFILITACVNFINLATAQALNRSLEVGIRKVLGGLSRQLFWQF
                     ITETAMIALLASALGYIVAASLLPSVNGLFKTQIGFDLLTDPAALAFGFVLLLVVIFL
                     SGSYPGLVLSRFQPVLALKSKLSQKQAGGFSLRRTLVVTQFAISQMLIIGTIVVAGQL
                     HFVGTADLGFTKDAIVLLPLPQHTNEKINMLGARIRELPGVETLSFCADAPASEGISS
                     SNIHFDNRKEDEHWEVSKKPGDADYLSTFGLHLAAGRNFFASDTTSEYLVNETMVKRL
                     NLRSPEEVIGKPMRINGHTGAIVGVVKDFNSSSLHGDIAPIALFPNASNYSTCAVKLN
                     PAYIHANLASLEKIWNQAFPDFLYSYGFLDDSIEKFYEADTSLLRMIEVFAAIAVFIG
                     CLGLYGLTSFMAVRKTKEIGVRKVLGAGIPGTLWLFGREFTQLVIIAFVIAAPISWWV
                     MHQYLQDFKYRITIGPGIFLLSIAATALIVLATVSYQSVRSALANPVKSLRSE"
     gene            3005..>4810
                     /locus_tag="VKQ52_12090"
     CDS             3005..>4810
                     /locus_tag="VKQ52_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ87981.1"
                     /translation="MLKNYLKTALRFLRNNRIFTFINIIGLSLGTLCCLYILLYIEQQ
                     YSYDKHEANAADIYRITTDMKISGDRLHTSWSSPPIAPALKKDFPEVRETTRAFDASI
                     FGAEEHLLIYKQRFFFEKEVLYVDSNFFDIFTYHFLEGNPAAALREPKTVVLNKTTAV
                     KLFGAEDPLGKTIRLSNKGGDETLKVTGIVDESLGKSHLHANVFFTMNTGLIGDIILH
                     RMTNWAGGNLLWSYVKLQPGADPKTFTAKLPSFLNGHGAQDLKSLGIVKQLSLQPIAT
                     IHTTAGYDHELQAPVSPSFLGLLLLIAVLIQVIACINFMNLSTARASKRAKEVGVRKV
                     IGAARGNLIGQFLGESFLLSLIGIALALPLLIICLPYLNSITHADITAAALKDGRVLL
                     LLTALVIGTGLISGSYPAFYLSAFNTVKVIKGNFTNRVSAAGLRKALVVFQFSLSIIL
                     ITGIVIIYGQLNYIKNKDLGYNTKQMLIFNSYAMGADLGAFTSGLRTLSGIKEVTRTN
                     SQLGIRPIQDQTVYLYGKTKADEQVAQNYVTDKYFLRTTGIQLLAGRDFHDYDSARVL
                     INVTLATRLGIDPAKAPGLKLHSSYDSTKTFEIIGV"
BASE COUNT         1088 a         1581 c         1062 g         1079 t
ORIGIN      
        1 acatcgacag gaagtcggcg tccactgtcg tcacggggat cgttatgtcc cgccccttgt
       61 attcgatatg attaccaccg tcgccaatcc gcgtcatccg ctcgatcgcc ggcgcctctg
      121 ctttataacc tggtccctgc ccaaggggaa atgcattctc aaaatgttcc ccggcaggat
      181 catgaaacac ctggtaggcc tcatacaggc gcttcccgct cacatgctga ctatcccact
      241 gaaattgctg gtacgtcagg agcaacgtgg cgatcccgat cgtaaacccg atgatcaggc
      301 cgccgatatt gattcccgta tgcaacttgt tgcggaacag attccgccag gcgactttca
      361 gatagttttg cagcatgtgt cagtggtttt ttgaagattg ggataactca ctctaaaaag
      421 atgcctaatt accaggggct taaagaccaa tattttatcg gcagacaacc cttcagcact
      481 gtccgcttcc gatacacccc atgatcacaa tcgcacaatc tacgaaaact atggtagtgc
      541 aaaaacttga aaaacaattg ggtagggaat tggtatcatc ttagtacaag cagcacccac
      601 aaccagcctg tatgctcaaa aacttctttg tcatcgcctg gcgcaacgtc cggaagaaca
      661 gggcctacgc ctccatcaat gtcctcggcc tctcccttgg catcgcctgt ggcctcctca
      721 tcttcacgct cgtccactac caccttagct tcgatacctt tcacgcggac agccgacgca
      781 tctaccgcat cgtcactgaa tggcacgacg aggcagctga ctactcccag ggaacccctt
      841 cgccgctggg aaaggccttc cgcaaggact tcgactacac ggaaaaggta gcacgcgtca
      901 ttgattttgg cgcgctcgtt accctccagg ataaggggga gacaaaaaaa ttctcggagc
      961 gcgtcgccta tgccgagccg gagtacttcg atatcttcaa cttcccgctt acccacggcg
     1021 acaaaaccac cgccctcgct gcccctggcc aggccctcct gtcggaaaag atggcgaaaa
     1081 aattctttgg cgatgctgcc gccgcgatag gccggacgat ccgcgttttt aatcaaacgg
     1141 acttccaggt cgtcggcatc ctccgggata tcccggtcaa taccgatcgc aaaacggaga
     1201 tttacctctc ttatggcagc ctgaaagacc atgatccgcg ccatgccagc gacagcagtt
     1261 ggggcagcgt ctatagtcag tgcatgacct ttatccggct gaaaccatcc gttaccgtcg
     1321 ctcagaccaa taaagcctta tccatcatca gcaaaaaata ccaggagggt agggacgccc
     1381 aaaccgttgt cttccggctc caacccctcg ccgatatcca tttcaatccc acctttgatg
     1441 gcatcgtgga caaaaaatac ctctgggcgc tggccttcat cggcttgttc attcttatca
     1501 ccgcctgtgt caacttcatc aatctcgcta ccgcccaggc actcaaccga agcctggaag
     1561 tgggcatccg gaaagtcctc ggtgggctga gccgtcagct cttctggcag ttcatcaccg
     1621 aaacagcgat gattgccctc ctggcttccg cgcttggcta tatcgtcgcg gcatccctcc
     1681 tgccctccgt caatgggctg tttaaaacac agatcggttt cgacctcctc accgatcccg
     1741 cagctctcgc ttttggcttt gtactgctgc tggtcgtgat cttcctttcc ggctcctatc
     1801 ccggcctcgt cctgtcccgc tttcagcccg ttctggcatt gaagagcaaa ctttcccaaa
     1861 aacaggcggg tggcttttcc ctccggcgta ccctggtcgt cacccaattc gccatctccc
     1921 agatgctgat catcggcacg atcgtcgtcg ccggccagct gcattttgtc gggacggccg
     1981 acctcggctt taccaaagac gccatcgtcc tcctgcccct tccccagcat accaacgaaa
     2041 agataaacat gctcggtgcc cggatccggg aattgccggg tgttgagaca ctctcttttt
     2101 gtgccgacgc gcccgcgtcc gaaggcatca gttcctccaa tatacacttt gacaatcgaa
     2161 aagaagacga acactgggaa gtaagtaaaa aacccggcga tgccgattat ctttccacct
     2221 ttggactgca tcttgccgcc ggccgcaact tctttgcttc cgacacgacc agcgaatacc
     2281 tcgtaaacga aaccatggtc aaaagactga accttcgctc acccgaggag gtcatcggga
     2341 agccaatgag gataaacggg cacaccggcg ccatcgtggg cgtcgtcaaa gatttcaaca
     2401 gctcttccct gcacggcgac atcgccccca tcgccctttt tcccaatgca agcaactatt
     2461 ccacctgcgc ggtcaagcta aaccctgcct atatccatgc caatctcgcc tccctggaaa
     2521 agatctggaa ccaggccttc cctgattttc tttactccta tggatttttg gacgacagta
     2581 tcgaaaaatt ttatgaggcc gacacttccc tcctgcgaat gatcgaggtc ttcgccgcca
     2641 tcgccgtttt catcggctgt ctcgggctct atggcctcac cagcttcatg gccgtccgca
     2701 agacaaagga gatcggcgta agaaaagtgc tcggcgccgg catcccgggg accctctggc
     2761 tgttcgggcg tgaattcacg cagctggtca tcatcgcctt cgtcatcgcc gcacctatca
     2821 gttggtgggt catgcaccaa tacctccagg atttcaaata ccgtatcacc atcggccccg
     2881 gtattttcct actttccatc gctgcaacgg cgcttattgt cctggcgacc gtcagctacc
     2941 aatccgtccg ctccgccctc gccaatcccg taaaatcatt acgtagcgaa taaactcact
     3001 gtctatgcta aagaactatc taaaaaccgc cctccgcttc ctgcgcaaca acaggatctt
     3061 caccttcatc aatatcatcg gtctctccct cggcaccctc tgctgcctgt acatcctgct
     3121 ctacatcgaa caacagtaca gctacgacaa acacgaagca aacgccgccg acatctatcg
     3181 catcacaacc gacatgaaga tctccggcga tcgcttgcat acatcctggt cctcaccccc
     3241 catcgcgcct gcactgaaaa aggacttccc cgaggttcgg gagaccactc gcgcattcga
     3301 tgcctccatc ttcggcgccg aagaacacct gctcatttat aagcaacggt tcttcttcga
     3361 aaaggaagtc ctctacgtcg actccaactt cttcgacatc ttcacctatc attttttgga
     3421 gggcaacccc gccgcggcat tgcgtgaacc caaaaccgtc gttctcaaca aaaccaccgc
     3481 ggtcaaactg ttcggtgccg aagatcctct cggcaagacc attcgcctca gcaacaaggg
     3541 gggtgatgaa acgctaaagg tcaccggcat cgtcgatgaa agcctgggca aatcgcactt
     3601 gcatgcaaac gttttcttca ccatgaatac gggcctcatc ggcgatatca tcctccaccg
     3661 gatgaccaac tgggccggcg gcaatctcct ctggagctat gtaaaactcc agcccggcgc
     3721 cgaccccaaa acgttcaccg caaaactacc ctctttcctc aacggtcatg gcgcccagga
     3781 tctcaagagt ctcggcatcg taaaacaact tagtctccag cccatcgcta cgatccatac
     3841 gacggccggc tatgaccatg aactgcaggc cccggtaagc ccgtcctttc tcggtctgct
     3901 ccttctcatc gccgttctca tccaggtcat cgcctgcatc aacttcatga acctctcgac
     3961 ggcccgcgcc tccaaacggg caaaggaagt cggtgttcgc aaggtcatcg gcgcggcacg
     4021 aggcaacctc atcggccagt tcctcggcga gtccttcctg ctctccctca tcgggatcgc
     4081 cctcgctctg cccctgctga ttatctgcct cccgtatctc aacagcatca cccatgccga
     4141 catcaccgcg gccgctttga aagacggccg ggtcctgctc ctgctcaccg ctctcgtcat
     4201 cggcaccggg ctcatttccg gcagctatcc cgcgttctat ctctcggcct tcaacactgt
     4261 gaaggtcatc aagggaaatt tcaccaaccg ggtctctgct gccggtctgc gcaaagcact
     4321 ggtcgttttc cagttctcgc tctccatcat ccttataacg ggcatcgtca tcatctatgg
     4381 ccagctaaac tatatcaaga acaaggacct aggctacaac acaaagcaga tgctcatttt
     4441 caactcctat gcaatgggcg ccgacctggg cgccttcacg agcggtctcc gcacgctttc
     4501 cggtataaag gaggttaccc gcaccaacag ccagctgggc atccgcccga tccaggacca
     4561 gaccgtctat ctctatggga agaccaaggc ggacgaacag gtcgcccaaa attatgtcac
     4621 ggacaaatat ttccttcgca cgactggcat ccaactcctc gccggccgcg acttccacga
     4681 ctacgactcc gcccgtgtcc tcatcaatgt caccctcgcc acgcgtctcg gcatcgaccc
     4741 ggcaaaagcc cccggtctga aactccattc cagttatgat tcgacaaaaa ccttcgagat
     4801 catcggcgtc
//
LOCUS       DATGFG010000646         3994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1605105, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000646 DATGFG010000000
VERSION     DATGFG010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605105"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3994
                     /locus_tag="VKQ52_12095"
     CDS             <1..>3994
                     /locus_tag="VKQ52_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HLZ87982.1"
                     /translation="DIGEFWASPAIGPGGTFTDAKSNLFVYSGGPDATIKFGAVGTAD
                     NPRTIQVSVNGNVAADTVMNSFNDLVTTRPVPLAQIAGGTATVGFQNSSTVSTDRMVV
                     SFYEINYPRQFNFGGAANFTFQLPARPAGYLLNITNVAASASTPVLYDMTNGYRYTAV
                     VNGSTLAFALNGSTATMNLVLVNEDPSTVQSVNSLTSKTFLNLANSANQGNYIIISNP
                     LLSTSSSGSNPLVDYKNYRSSATGGGFDAQVYDINELVDQFAFGIKKDPLSIQNFMRY
                     ARAKFATKPQYVLLIGHSMVYSDYYTYDQVNRDPLADVLNLVPTFGNPASDNKLSADD
                     GVGATPITPIGRLSVVSGDELETYLTKVKEYEQVQTTAPNTIAGRLWMKNGVHLTGVS
                     EPYLGTIICNYMTYYGSIISDTLTGASISLFCDGNASQVSQVPVSVISNLFATGLSFL
                     DYFGHSSNTVLGYNLDNPNDYNNQGKYPIFFINGCDAGNFFIYDDHRPGGTSKTLSET
                     YVLAPERGSIAFVASTNFGIVNYLNILLTGLYNLMDGSDYGKPLGILQKDALNGLVNA
                     VPGDFFARLHAEQMTTHGDPYIKLNQGPTDYDIESSQVTINPTLVSVSNTVFTVSARI
                     YNLGKAVSDSVQVTVTRTFPNGTSALLLSKKLKGIRYVDSIQLAVPIVASRDKGVNKI
                     TITVNPANVIPEVTYANNSVTTDVFIYQDGATPVYPYNYAIINTPTSKLIASTANPLI
                     PSTQYVMELDTTQNFNSSLKVDKYLTSVGGELEFDPGVNFMDSVVYYWRVSPVPTTPT
                     GVYTWNYSSFIYIDPARSSTGMNQSHYFQHQGSTADSMQIAASRQWTFGTTTHNFYIT
                     NAMYPTSGIYEQDFALGVDGNSYIQSACIGHSLIFNVFNPITLRAWLNVDPSTGNNLY
                     LSGSAAANCGPQRWWNFEFSYESAASRYLMTRFMDSIPAGYFVVVRSFDYDASGGSYC
                     PNWRADTSLYGSGKSLYHYLLNAGFADVDSITFPRDWQFIYKKGDPSFVPQYKISQGL
                     FDKITQSLDASAPLYSGTVISPLFGPSKKWSQVHWRGHDPVTPVTDTVGVQIVGIDTL
                     GNFSPLYNLSRGVQDFDVSAVNARQYPYLQLRMTTLDTVHAKPFQLDYWRVNYTPVPE
                     GAIAPNILLKAPDTVALGQPIEFAIAFKNVSPFNFDSMRVKLYLQDRSGAFHTITLPR
                     KKPLVTGDTVAIDYTFDSKPYSGANTIYVGFNPDNDQPEQYLGNNFVYKPVFVKGDSR
                     NPNLDVTFDNVHILNEDIVSAKPHIQIKLQSQSQYLLLTDTSLVNVQVKYPDGSLHTY
                     RFNTDTLRFTP"
BASE COUNT          874 a         1019 c         1138 g          963 t
ORIGIN      
        1 cgatatcggg gagttttggg cttcaccggc cattggtccg ggtggaacct ttacggatgc
       61 caagtcgaac ctttttgttt atagcggcgg ccctgatgcg accatcaaat tcggggcggt
      121 gggtacggcc gataacccgc ggacgataca ggtgtccgtc aacgggaacg tcgctgcgga
      181 taccgtgatg aacagtttca acgacctggt gacgacccgg ccggtgccgc ttgcacagat
      241 cgccggaggg actgcgacgg tggggtttca aaacagttcc acggtgagca cggaccggat
      301 ggttgtttcg ttctatgaga tcaattatcc ccggcagttc aattttggcg gggcggccaa
      361 ttttaccttt cagttgcctg cccggccggc gggttacctg ctgaatatta ccaatgttgc
      421 ggcatcggca tcgacccctg tgttgtatga tatgacaaat gggtatcgtt ataccgcggt
      481 ggtaaatggc agtacgcttg catttgcgct gaacgggtct accgcgacga tgaacctggt
      541 gctggtgaat gaggaccctt cgacggtgca gagcgtcaac agcctgacgt caaagacctt
      601 cctcaatctt gccaattccg ccaaccaggg gaattatatc attatcagta atccgcttct
      661 ttccaccagc agcagtggca gcaaccccct ggtggactat aagaactatc gcagcagtgc
      721 gacgggtggc ggttttgacg cgcaggtgta cgatatcaac gagctggtgg accagttcgc
      781 ttttgggatc aagaaggatc ccctttccat ccagaatttc atgcggtatg cgcgggcgaa
      841 atttgcgaca aagccgcagt atgtcctgct gatcgggcat tcgatggtgt attcggatta
      901 ctatacctat gaccaggtga accgcgatcc gctggccgac gtgctcaatt tggtgcctac
      961 tttcggtaat ccggcgtccg acaacaagct tagcgccgac gacggggttg gcgccacccc
     1021 tatcacgccg atcggacggt tgtccgtcgt ctccggggat gagttggaga cgtatctgac
     1081 caaggtgaag gagtatgagc aggtgcagac cacggcgccg aataccattg ccggccggct
     1141 gtggatgaaa aatggggtgc acctgacggg ggtgagcgag ccctacctgg ggaccatcat
     1201 ttgtaattac atgacctatt acggcagtat tatttcggat acgcttacgg gcgccagtat
     1261 atccctgttc tgcgatggga acgccagcca ggtgagccag gtgccggtga gtgtcatttc
     1321 caacctgttc gcgacggggc ttagctttct ggactatttc ggtcactctt cgaatacggt
     1381 acttggttat aacctcgata acccgaatga ttataacaac caggggaaat acccgatctt
     1441 ttttattaat ggttgtgatg cgggtaactt ctttatctat gatgaccacc ggcccggtgg
     1501 gaccagcaag actttgtcgg agacttatgt actggcgccg gagcgcgggt cgatcgcctt
     1561 tgtggccagt accaacttcg ggatcgtcaa ttacctgaat atccttttga cgggtttgta
     1621 taacctgatg gatgggagtg attacgggaa gccgcttggc atcctgcaaa aagatgcgct
     1681 gaacggcctg gtcaacgcag tgccgggcga cttttttgcc cggttgcatg cggagcagat
     1741 gacgacgcat ggggacccct atatcaagct gaaccagggg ccgaccgact atgacatcga
     1801 gtcttcgcag gtgacgatca atcccacgtt ggtgtcggtg agcaataccg tatttacggt
     1861 gagtgcgcgg atctataacc tggggaaggc ggtttcagac agtgtacagg tgacggtgac
     1921 ccggaccttt cccaatggga cttccgccct gttgttatcg aagaagctga agggcatccg
     1981 ttatgtggac tcgatccaac tggccgtgcc gatcgttgcg tcgagggaca agggggttaa
     2041 taagatcacg atcacggtga acccggctaa tgttattcca gaggtgacgt atgcgaataa
     2101 ttcggtgacg acggatgtgt tcatctacca ggatggggct acgcctgtct atccttacaa
     2161 ctatgcgatc atcaatacgc cgacttcgaa gctgatcgcc tcgacggcga acccgctgat
     2221 cccgagtacg cagtatgtga tggagctcga tacgacgcag aacttcaact cttcgttgaa
     2281 agtggataaa tacctgacct ccgtgggtgg tgaactggaa ttcgacccgg gggtaaactt
     2341 tatggacagc gtcgtgtact actggcgggt ctcacccgta ccgacgacac cgacgggtgt
     2401 ctatacctgg aactacagtt cattcatcta tatcgatcct gcgaggagca gcacgggaat
     2461 gaaccagtcc cattatttcc agcatcaggg ttcgacggcg gatagtatgc agattgccgc
     2521 cagccgtcag tggacctttg gcactaccac gcacaatttc tatatcacga acgccatgta
     2581 cccgacatcg gggatatatg agcaggattt tgcgcttggt gtggatggga actcctatat
     2641 tcagagcgct tgtatcggtc actcgcttat cttcaacgta ttcaacccga ttacgctgcg
     2701 ggcctggctg aacgtggatc ccagtacggg gaataatctt tatctcagcg ggagcgctgc
     2761 ggcgaactgc ggaccccagc gctggtggaa tttcgagttc tcctatgaaa gtgcggcgag
     2821 ccgttacctg atgacgcggt tcatggacag tatccctgcc ggctatttcg tggtggtgcg
     2881 gagctttgac tatgatgcga gcggcgggtc ctattgtccc aactggcggg cggatacctc
     2941 cctgtatggc agcggcaagt ccctttatca ttacctgttg aatgccgggt ttgccgatgt
     3001 cgattccatt accttcccgc gggactggca attcatctat aagaagggtg atccttcttt
     3061 tgtaccgcag tataagatat cgcaagggct ttttgacaag atcacccagt cattggatgc
     3121 ttctgctccg ctgtacagcg ggacggtgat ctcgccgttg ttcgggcctt cgaagaaatg
     3181 gagccaggtg cattggcggg ggcatgatcc cgttacgccg gtgacggata cggtgggcgt
     3241 gcagatcgtg gggatcgata cgctcgggaa tttcagtccg ttgtataacc tcagccgggg
     3301 ggtgcaggac tttgatgtat cggccgtgaa tgccaggcaa tacccctatc tgcaactgag
     3361 gatgacgacg ctggataccg tgcatgcgaa acccttccaa ctggattact ggcgggtgaa
     3421 ttacacgcct gttccggaag gcgccatcgc cccgaatatt cttttgaagg ccccggatac
     3481 cgtggcgctt ggtcagccga tcgagtttgc catcgccttt aagaatgtga gccccttcaa
     3541 tttcgacagt atgcgggtga agctgtatct gcaggaccgg agcggcgcct tccatacgat
     3601 caccctgccg cggaagaagc cgctggtcac gggtgatacg gtggcgatag attatacatt
     3661 cgattccaag ccttattccg gcgccaacac catctatgtg ggtttcaacc cggataatga
     3721 ccagccggag caatacctgg ggaataactt tgtgtacaag cctgtttttg taaaaggaga
     3781 cagtcgcaat cccaatctcg atgtcacctt cgacaatgtc catatcctga acgaagatat
     3841 cgtttcggcc aagccgcata tacagatcaa gttgcaaagc cagtcacagt atttgttgct
     3901 gacggatacg tcgcttgtga atgtgcaggt gaaatacccg gatgggtcgc tgcatacata
     3961 ccggttcaac accgacacgc tgcggttcac ccct
//
LOCUS       DATGFG010000647         6580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1555779, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000647 DATGFG010000000
VERSION     DATGFG010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555779"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1145)
                     /locus_tag="VKQ52_12100"
     CDS             complement(6..1145)
                     /locus_tag="VKQ52_12100"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HLZ87983.1"
                     /translation="MRMIMRPIQMVDLKRQYTKIKSEVDLAIQEVLDTAAFINGKAVT
                     DFAAGLAAYTGSKHVIPCANGTDALQIAMMALDLQPGDEVITPSFTYVATTEVVALLK
                     LKPVFVEVDPKTFCIDPAAIEKAITPRTRAIVPVHLYGQSAPMREIVDIAARHNLYII
                     EDNAQAIGSTYRFADGTVKKTGSIGTIGTTSFFPSKNLGCYGDGGAIFTDDDTLASRL
                     KMIANHGQSRRYYHDLVGCNSRLDSIQAAVLNVKLKRLDEYITARRAAADHYDKAFNA
                     HPKITTPYRAPYSHHVFHQYTLVLEGVDRNGLNEFLAGRNIPSMIYYPVPAHRQQMFA
                     GYGGGNYHLETTDWLTERVISLPMHTELDDEQLQTISDAVLEFIK"
     gene            1247..2536
                     /locus_tag="VKQ52_12105"
     CDS             1247..2536
                     /locus_tag="VKQ52_12105"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HLZ87984.1"
                     /translation="MSIIFYHVFLLLYKTGIRLISPWNRKARLWLEGRQGLFDRIRAE
                     MQAAAGKPGPVIWMHCSSLGEFEQGRPVLEELRRQSPGCRIVLSFFSPSGYTTRKDYK
                     GADHIFYLPLDSPGNARLFIDLVKPTIVLWVKYDYWYYFLVELKKRRIPLLLISGIFR
                     EDQPFFKWYGRLHRYMLECFTHLFVQTEASKLLLRRLRLAENVTVSGDTRFDRVIEIA
                     ASGEPLPLIEAFCGRQPVIVAGSTWEEDEEELDHYANTHPDIRFIVAPHEISEARLQE
                     VEGLFRHCIRFSVLAKGAVPPAAGRGAWPEPNVLIIDNIGMLSRLYRYATVTYVGGGF
                     GDDGVHNVLEAAVYGKPVVFGPVIEKYIEAVELTDSGGGLIIDSALEAEKVFNRLLQN
                     PEECRETGEASSRYVHSKKGATEQIVRYIQENRLLTN"
     gene            complement(2509..3369)
                     /locus_tag="VKQ52_12110"
     CDS             complement(2509..3369)
                     /locus_tag="VKQ52_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87985.1"
                     /translation="MERLKELITQLKEQFDRKVDPAQMLDTTRLLEAELQLLASQPQP
                     VRVMPTKIAVMMPSATKMYAGQQPAPSNPEPQKDLAAQRNNGTNGSYPEQKKEPAPTP
                     APAPANPPVTAAGPDTPDSRYMPPAPPPVVVPEPNPAVTIAATATLEKPLERGQWPFN
                     PLADKPIDRFTLSQRAREVNDVIGFASTSLNDKLKSDVSDLKSALNDTPVRDLRKAIG
                     VNDRYVFINQLFRGDEVMYERSLKTINGFRILPEAEYWMERELKVKLGWDENREATRH
                     FYQLVKRRFS"
     gene            3458..4027
                     /locus_tag="VKQ52_12115"
     CDS             3458..4027
                     /locus_tag="VKQ52_12115"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HLZ87986.1"
                     /translation="MFIEPHLKGERRGWIEVICGSMFSGKTEELIRRLKRAKIANLKV
                     EIFKPAMDTRYHEQHIISHDENAIQSTPIDNSQTILLLAGEVDVIGIDEAQFFDDQLP
                     EVCDQLAVRGIRVIVAGLDMDYLAKPFGQMPYLLAKAEYITKLHAICVKCGNIANYSY
                     RKTAKTGQFLLGEKEAYEPRCRHCYYTLA"
     gene            4088..4750
                     /locus_tag="VKQ52_12120"
     CDS             4088..4750
                     /locus_tag="VKQ52_12120"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HLZ87987.1"
                     /translation="MTEHIKNIINLLKKDLLLEIRQQYTFYGVLLYVVSTIFVLYLSM
                     GQPEGPVWKGLFWMIQLFICVNAVAKSFLQEGRGRLLYFYSIAGARDFILSKLLFNAL
                     LMLVMSLLSLALFLFMLGNPMGHEGYFILISCLGGVSLSLVFTFLAALAARAQQGAAL
                     MAILGFPLVIPQLMLLNRVSTIAFEDVLQGGLGWMLAILFGLDVLLIALALILFPFLW
                     KD"
     gene            4825..5493
                     /gene="ccsA"
                     /locus_tag="VKQ52_12125"
     CDS             4825..5493
                     /gene="ccsA"
                     /locus_tag="VKQ52_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLZ87988.1"
                     /translation="MLRQTWWKILCVLLLAYTVIGGFLGPVPAKPIVNETIRNLYFHV
                     AMWFSMMVFLTVSAVYAIRYLRRPNHVDDIYSLEYAKAGIVFGTLGLITGSEWAGFTW
                     GAFWSNDPKQLGAMIAMLIYYAYLVFRNGMPDIDKRARLGAVYNIFAFAMLFPTLWIL
                     PRLVESLHPGGLGGNPAFKDSDPRMAAVFWGGAVPGWCLLGVWITTLRIRLKLLAEKK
                     LSNI"
     gene            5496..5720
                     /locus_tag="VKQ52_12130"
     CDS             5496..5720
                     /locus_tag="VKQ52_12130"
                     /inference="COORDINATES: protein motif:HMM:NF040113.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87989.1"
                     /translation="MYKKFWVLLLSCWMLTLSAMAQNDTTRPVEMADGLRANGKIYVV
                     VMVLVTILAGLIFYVVRLDRKISRLEKENK"
     gene            5775..>6580
                     /locus_tag="VKQ52_12135"
     CDS             5775..>6580
                     /locus_tag="VKQ52_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HLZ87990.1"
                     /translation="MEYKFFEAVEKSFDKAAAFTNWDPGILEQIKQCNAVYRMFFPVK
                     IGDKIEVIKAYRVQHSHHKTPCKGGIRFAPSVNLDEVMALAALMTFKCAIVNVPFGGA
                     KGGISIDPKKYTPYELEKITRRYTSELIKKNFIGPGTDVPAPDYGTGEREMAWILDTY
                     MSMRPGEIDALGCVTGKPVSQGGVRGRREATGLGVFYGIREVCNMEEIMKKQGLKTGV
                     EGKTVVVQGLGNVGYHAAKFFREGGARVIALAEFEGAIMNPAGLNEEEVF"
BASE COUNT         1359 a         1549 c         2033 g         1639 t
ORIGIN      
        1 aatatttatt ttatgaattc gagaacggca tcgcttatcg tctgcagttg ctcatcgtct
       61 aattctgtgt gcatgggaag ggagatgacc cgttccgtca gccaatcggt agtttcgagg
      121 tggtaatttc ccccgccata gccggcaaac atctgctggc ggtgagcggg tacgggataa
      181 tagatcatcg acgggatatt ccggccggcc aggaattcat tcagtccgtt gcggtcgact
      241 ccttcgagga ccagggtata ctggtggaat acatggtggc tgtagggcgc ccggtagggc
      301 gtcgttatct tcgggtgagc gttgaatgct ttatcataat ggtcggcggc ggcacgtctg
      361 gcggtgatgt actcgtccag tcttttcagt tttacattca acacggcggc ctggatggaa
      421 tcgaggcggc tgttacagcc taccagatcg tgataatagc gcctgctctg cccatggtta
      481 gcaatcattt tcaaccggct agctaacgtg tcatcatcgg taaatattgc tccgccgtcg
      541 ccataacacc ccaaattttt tgacgggaag aaggaggtgg tgccgatggt cccgatggag
      601 ccggtctttt ttaccgttcc gtcggcgaag cggtatgtac ttccgatcgc ctgggcgttg
      661 tcttcgataa tatacaggtt atggcgggcg gcgatgtcca cgatctcccg catgggagcg
      721 gactggccgt agagatgaac gggcacgatg gcgcgggtgc ggggggtgat cgctttttcg
      781 atggcggcgg ggtcgataca gaaggttttc gggtcgacct ctacgaatac gggtttcagt
      841 ttgaggaggg ctacgacctc ggtggtggcg acataggtga aagagggggt gatgacctcg
      901 tcaccgggct ggagatcgag ggccatcatg gcgatctgca gggcgtcggt gccgtttgcg
      961 cagggaatga cgtgtttcga ccccgtatat gcggcgagtc cggcggcaaa atcggtcacc
     1021 gccttcccat tgatgaatgc cgcagtatcc agcacctcct gaatggccaa atcaacttca
     1081 ctctttattt ttgtatattg ccgctttaga tctaccattt ggatgggccg cataatcatt
     1141 cgcattttag acgggcacaa acctactgca aaaatgtggt ttatccaatg ctacgaatgg
     1201 gtagcgccgc ccggtggcgg ccggatacgg ggtattatta tatgatatga gtattatttt
     1261 ttatcatgtt tttcttctac tgtataaaac aggcattcga ctgatctctc catggaacag
     1321 gaaggccagg ctttggctgg agggaaggca ggggcttttc gaccggataa gagcagagat
     1381 gcaggctgcg gcgggcaagc cggggcccgt gatctggatg cattgttctt cgcttggaga
     1441 gttcgagcag gggcggccgg tactggagga actgcggcgg cagtcgccgg gttgccgtat
     1501 tgtgcttagt ttcttttcgc cctcggggta tactacccga aaagactaca agggggccga
     1561 tcatattttc tatctgccat tggacagccc gggaaatgcc cggttgttca tcgacctggt
     1621 gaagcctacg atcgtattgt gggtgaagta tgattactgg tattattttc tcgtggaatt
     1681 gaagaaaagg aggatccccc tgttgctgat ttcggggatc ttccgggagg accagccttt
     1741 tttcaaatgg tatggccgcc tgcaccggta tatgctggaa tgttttacac acctttttgt
     1801 gcagacggag gcgtcgaagc tattgttgcg gcggttgcga ttagcggaga acgtcacggt
     1861 cagcggggat acccggttcg accgggtgat cgagatcgct gcatcggggg agccgttgcc
     1921 cctgatcgag gctttttgcg gccggcagcc ggtgatcgtg gcaggttcca cctgggagga
     1981 ggacgaggag gagctggatc actacgccaa tacgcacccg gacatccggt ttatcgtggc
     2041 gccgcatgag atcagcgagg cccggctcca ggaggtggag ggtcttttcc ggcattgcat
     2101 ccggttttcc gtgctggcaa aaggggccgt tcccccggcg gcaggaaggg gggcatggcc
     2161 cgagccgaat gtgctgatca tcgacaatat cgggatgctg agccggttgt accggtatgc
     2221 caccgtcacc tatgtgggag gaggatttgg ggatgatggg gtgcacaatg tgctggaggc
     2281 ggcggtttat ggcaagccgg ttgtttttgg gccggtcatc gagaaatata tcgaggcggt
     2341 ggaactgacg gacagtggcg gagggttgat catcgacagt gcgctggagg cggaaaaagt
     2401 gtttaaccgg ctgttacaga acccggaaga atgccgggag acgggggagg cctcgagccg
     2461 ttatgttcat tcgaagaagg gagctacgga acagatcgtc cggtatattc aggagaaccg
     2521 tcttttaacc aattgataga aatggcgggt ggcctcgcgg ttctcatccc agcccagttt
     2581 taccttcaat tccctttcca tccagtattc ggcttcgggt agtatcctga agccattgat
     2641 cgtcttgagg ctgcgttcat acatgacctc gtcgccgcgg aagagctggt tgataaagac
     2701 atagcggtcg ttgaccccga tggccttcct gaggtcgcgg acgggggtat cgttgagtgc
     2761 ggatttgaga tccgacacat cggatttcag cttgtcgttg agggaggtgc tggcaaagcc
     2821 gatgacatcg ttcacttccc gggcgcgctg ggagagggta aagcgatcga tcggtttgtc
     2881 tgccagggga ttaaagggcc actggcctct ttccaggggt ttctccaggg tggcggtggc
     2941 ggcgatcgtc acggccggat ttggctcggg cactaccacc ggaggaggag cgggcggcat
     3001 ataccgggaa tccggggtat ccggcccggc ggcggtgacc ggcgggttgg ccggggccgg
     3061 ggcgggagtc ggagcgggtt ccttcttttg ttcgggatag ctgccgttgg tgccgttatt
     3121 tcgttgggct gcaaggtctt tctggggctc gggattggac ggggcaggtt gctgtccggc
     3181 atacattttg gttgcggatg gcatcataac cgctattttt gtcggcatta ccctgacggg
     3241 ttggggctga ctggccaaaa gctgcaactc ggcttcgagg agccgggtgg tatcgagcat
     3301 ctgagcagga tcgacctttc ggtcgaactg ctccttcaat tgggtgatca gttctttaag
     3361 tcgttccatg gtacaacaag ctaaacagca ctttaagggt tagtagaaga acgtatataa
     3421 agttataaca aattccgaac ctaatttcta cctatctatg ttcatagaac cccatcttaa
     3481 gggtgagaga cgcggctgga tcgaagtcat ttgcggatcg atgttctcgg ggaagaccga
     3541 agaattgatc aggcggttaa agcgtgctaa aattgctaat ctaaaagttg agatctttaa
     3601 acctgccatg gatacccgct atcatgagca gcatatcatt tcgcatgatg aaaatgctat
     3661 ccaatcgacg cctatcgaca attcgcagac catcctttta ttggcggggg aggtcgatgt
     3721 catcggcatc gacgaggccc agttcttcga cgaccaattg ccggaagtct gcgaccagct
     3781 tgcggttcgt ggcatccggg tgatcgtcgc cggtcttgat atggactacc tggctaaacc
     3841 ttttggccag atgccttacc tgctggctaa agctgaatac ataaccaagt tgcatgccat
     3901 ttgtgtcaaa tgtggaaaca ttgccaacta ttcctaccgc aagacggcga agacagggca
     3961 gttcctgttg ggtgaaaagg aggcttatga gccgcgctgc cgccattgtt attacaccct
     4021 ggcgtgaaaa accggcctcc cggggggcca tcggacaagc aaaaattaac gaatcatttg
     4081 gttttttatg accgaacata ttaagaatat tataaattta ttaaaaaagg accttttact
     4141 cgagatccgg cagcagtata ccttttacgg ggtgttatta tatgtggtgt ccacgatctt
     4201 cgtgctgtac ctgtcgatgg ggcagccgga gggaccggtg tggaaggggc ttttctggat
     4261 gatccagcta tttatatgtg taaatgcggt ggccaagagt tttttgcagg agggccgggg
     4321 acggttgcta tatttctatt cgatcgcggg agcgcgcgat tttattcttt ccaagctgct
     4381 ttttaatgcc ctgctgatgc tggtgatgag tctgctcagc ctggccctgt tcctttttat
     4441 gctgggtaac ccgatgggcc atgaaggata cttcattttg atctcctgtc ttggaggggt
     4501 gagcctgagc ctggtgttca ccttcctggc ggcgttggcg gcgagggcgc agcaaggtgc
     4561 ggcgctgatg gccatcctgg gtttcccgct ggtgatccct cagctgatgt tgctgaaccg
     4621 ggtgtcgacc attgcgttcg aggatgtgtt gcagggaggg ctcggctgga tgctggcgat
     4681 cctcttcggg ctggatgtct tgctgatcgc actggccctg attctttttc catttttatg
     4741 gaaagactga atattttcct cctacctttg ccctccaaac ggggtatcct gtaaggccgg
     4801 gatcggcccc tcaagaagaa atctatgctt cgtcagacct ggtggaaaat attgtgcgtc
     4861 cttttattag cgtatacagt catcgggggc tttctgggcc ctgttcccgc caaacctatc
     4921 gtcaacgaaa cgatccgaaa tttatacttc cacgtcgcca tgtggttttc gatgatggtc
     4981 tttctcaccg tctccgcggt gtatgccatc cggtacctcc gcaggcccaa tcatgtggat
     5041 gatatctatt cgcttgaata tgcaaaggcg gggatcgtct tcggcacgct cgggctcatt
     5101 acggggagcg agtgggccgg gtttacctgg ggcgctttct ggagcaacga ccccaagcag
     5161 ttgggggcga tgatcgccat gctcatctac tatgcctacc tggtgttccg caacggtatg
     5221 ccggatatcg ataagcgggc aaggcttggc gcggtgtaca atatttttgc gttcgcgatg
     5281 ttatttccca cgctatggat cctgccgcgg ctggtggaga gtctgcatcc gggcgggttg
     5341 ggcggcaatc cggccttcaa ggacagcgat ccgcggatgg cagcggtatt ctggggcggc
     5401 gccgttccgg gctggtgcct gcttggggtt tggatcacga ccctgcgtat ccgcctgaag
     5461 ctgcttgccg aaaaaaaact ttctaatatt taagaatgta taagaaattt tgggttctcc
     5521 tgctctcctg ttggatgctg accttgtctg cgatggcgca gaacgatacg accaggccgg
     5581 tcgagatggc cgatgggctc cgggccaacg gtaagatcta tgtcgttgtg atggtgctgg
     5641 tgaccatcct cgcgggtctg atcttttatg tggttcggct ggacaggaag atcagcaggc
     5701 tggaaaagga aaataagtag tcacacatgg gcgttggcca tgcaggcttc gtcctactta
     5761 aatatgcaag ccaaatggaa tacaaatttt ttgaagccgt agagaaaagc tttgataaag
     5821 cagcagcttt taccaattgg gaccccggca tcctggagca gataaagcaa tgtaacgccg
     5881 tgtatcgtat gttcttcccc gtcaagatcg gggacaagat cgaagtgatc aaggcctacc
     5941 gggtacagca ctcgcatcac aagacgccct gtaagggcgg catccggttc gcaccttcgg
     6001 tgaacctgga tgaagtgatg gcgctggctg cgctgatgac ctttaaatgc gccatcgtga
     6061 atgtgccctt tggcggcgcc aagggcggga tcagtatcga tccgaagaag tacactcctt
     6121 atgagctgga gaagatcacg cggcggtata cctccgaact gatcaagaag aactttatcg
     6181 gtccgggtac ggacgtcccc gctcccgact atggtacggg tgagcgggag atggcctgga
     6241 tcctcgacac ctatatgagt atgcggcccg gggagatcga tgccctcggt tgtgtaacgg
     6301 gcaagcctgt cagccagggt ggcgtcaggg gccggcgtga ggccacgggt ctcggcgtct
     6361 tctacgggat ccgcgaggtc tgcaatatgg aggagatcat gaaaaagcag ggactgaaga
     6421 ccggggtgga aggaaagacc gtggtggtgc aggggcttgg caatgtgggt tatcatgccg
     6481 ccaagttctt ccgtgaaggc ggggccaggg tcattgcact ggccgagttc gagggcgcga
     6541 tcatgaaccc ggcgggattg aacgaggaag aagtgttcca
//
LOCUS       DATGFG010000648         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_935983, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000648 DATGFG010000000
VERSION     DATGFG010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935983"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VKQ52_12140"
     CDS             complement(<1..1229)
                     /locus_tag="VKQ52_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLZ87991.1"
                     /translation="MSFQHLNLIEPLLKALNDEGYTTPTPIQQQAIPIILERKDLLGC
                     AQTGTGKTAAFALPLLQLLCEDAAGVKETTRTGGHRPVRALILTPTRELAIQIDESFA
                     AYGKYTGLKHTVIFGGVPQHSQVQTLQRGVDILVATPGRLLDLCDQKLLHLKDIHYFV
                     LDEADRMLDMGFINDVRKILTRLPQRKHSLFFSATMPPEIQKLAGSILVNPSKVEVTP
                     VSSTAEAIQQSIYFVDKKDKRSLLLHILHNHAIPTALVFTRTKHGADKVAQGLSRAGI
                     RAEAIHGNKSQNARQRALENFKSRHTRILVATDIAARGIDIDDLTHVINFELPNVPET
                     YVHRIGRTGRAGASGTALSFCDGEEKEYLRDIQKLIARQIPVVEDHPHVMHASLATHS
                     PAPEAVVARAEANRTNHG"
     gene            complement(1583..1804)
                     /locus_tag="VKQ52_12145"
     CDS             complement(1583..1804)
                     /locus_tag="VKQ52_12145"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HLZ87992.1"
                     /translation="MKQYLSLLLRLDQFIRQKGTGSPPEFARKMGISERSLYEYLKVL
                     KDLGAPIRFSRQDHSYYYEIDGQFHVSFL"
     gene            complement(2009..>2620)
                     /locus_tag="VKQ52_12150"
     CDS             complement(2009..>2620)
                     /locus_tag="VKQ52_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HLZ87993.1"
                     /translation="SSAPHFYVTMSIDMDKAVEARGKLNEVSPVKISFNDIVLKAVAV
                     ALKQHPAVNSSWLGDKIRINHHVHIGVAVAIEDGLLVPVVRFADTKTLSQISTEVKDL
                     AQKAKNKKLQPADWEGSTFTISNMGMFGVDEFTAIINPPDACILAISSIQQVPVVKNG
                     AIVPGNVMKLTLSCDHRVVDGATGSAFLQTVKSLLEEPLRMMI"
BASE COUNT          588 a          663 c          726 g          643 t
ORIGIN      
        1 ccgtggttgg tgcggttggc ttccgcgcgg gcgacgaccg cttccggcgc cggggaatgc
       61 gtggcgagag aggcatgcat cacatgcgga tgatcttcca ccaccgggat ctggcgggcg
      121 atcaacttct ggatatcccg caggtattct ttttcctccc catcgcaaaa ggagagggcc
      181 gtgccgctcg cacccgctct accggtccgg cctatgcggt gaacataggt ctccggtaca
      241 ttgggcagtt cgaagttgat gacatgggtg aggtcgtcga tatcgatgcc tctcgccgca
      301 atgtccgtgg ccacgaggat gcgtgtatgc ctgctcttga agttctccag cgcccgctgt
      361 cttgcattct gcgatttgtt gccgtggatc gcttcggcgc ggataccggc gcgggaaagc
      421 ccctgggcta ccttgtccgc cccgtgcttg gtccgggtaa agaccagcgc cgtgggaata
      481 gcgtgattat gcaggatatg cagcaggagg ctccgcttgt ccttcttgtc gacgaagtag
      541 atcgattgtt gaatggcctc ggccgtggaa gacaccgggg tgacttccac cttggacggg
      601 ttcaccagga tggagcccgc taatttctgg atctccggcg gcatcgttgc cgagaagaac
      661 agggagtgtt ttctctgcgg aagccgcgtc aggatcttcc ggacgtcgtt gataaaaccc
      721 atatccagca tgcggtcggc ctcgtccaat acgaagtaat gaatatcctt gaggtggagg
      781 agtttctggt cgcaaagatc gagcaatctg cccggggtgg caacgaggat gtctactccc
      841 cgctggaggg tctgcacctg ggagtgctgg ggcaccccgc cgaagataac cgtatgtttt
      901 agaccggtat acttgccata ggcggcgaag ctctcgtcta tctggatagc cagttcacgg
      961 gttggcgtaa ggatcaatgc cctgacgggg cggtgccctc cggtcctggt cgtctctttt
     1021 acccctgccg catcttcgca tagcaattgg agtaagggaa gggcgaaggc cgcggtcttg
     1081 ccggtgccgg tctgggcgca gccaaggagg tcttttcgtt ccaggatgat cggtatagcc
     1141 tgttgttgga taggtgtagg tgtcgtgtag ccctcatcgt tcaaagcctt taacaaaggc
     1201 tcaatgaggt tgagatgttg aaatgacaaa atgatttcta tttaaattga aaaaaggccc
     1261 atctactcag caatatcctt caggagtatg tacagaaggt tgtcacaaaa agtgattaga
     1321 aaggaatata acggttgcaa aggtagacta tccggttcaa atggccaaaa tgactatttc
     1381 ggggttttta aaagattttt tgcggtcttt ttggtccgca ttcgtttttg tactatattt
     1441 gattcaacaa attttttcta acagttaaag tttggttgga acagcaagga cacagttctt
     1501 gagagccctt tgtttctaca gaggcctcga aaggataagg tttaaagggc cgcctcatgt
     1561 ctatgaggcg gctttttgtg cattagagga aactgacatg aaattgcccg tcgatctcgt
     1621 agtagtagga atgatcctgc cgggagaacc ggatcggggc gcccaggtcc tttagaacct
     1681 ttaggtattc ataaagactt cgttcggaga tacccatctt acgggcaaac tccgggggag
     1741 accccgttcc tttttggcgg atgaattggt ctaatcgcag gagaaggcta aggtattgct
     1801 tcataaaagg tgttttttga cgccaccgct cagaagcatt aaaccatata aaaaagccac
     1861 ttaaaaatac gggagtctcc gaaataagcg gaggagtccg tccaaaaaat aaaggccggt
     1921 aaatgctaac actcatcggc ctttatacaa atttatttaa accaaaacgg accggaggcc
     1981 cgccggccca aggccacatc ggcctttcct agatcatcat acgcagcggc tcctcgagca
     2041 ggctctttac cgtctgcagg aatgccgaac cggtggcgcc atccactacc cggtgatcac
     2101 agctcagggt cagcttcata acgttaccgg gtacgatcgc cccattcttc accaccggca
     2161 cctgctggat cgaactgatt gccaggatac aggcgtccgg cggattgatg atggccgtaa
     2221 actcatccac gccgaacatc cccatattag agatagtgaa ggtgctgcct tcccagtcgg
     2281 caggctgtaa cttcttattt tttgcctttt gggccaggtc ttttacttca gtggaaattt
     2341 gtgaaagcgt cttcgtgtcg gcgaagcgga ccaccggcac aagaagacca tcttcgatgg
     2401 cgactgccac gccgatgtgc acatgatggt tgatgcggat cttatcgccc agccagctgc
     2461 tgttcactgc cggatgctgc ttcagggcca cggccaccgc cttcagcacg atatcgttaa
     2521 aggagatctt caccggggat acctcgttca acttaccccg ggcttctacc gccttgtcca
     2581 tgtcgataga catcgttaca tagaaatgcg gcgcggaaga
//
LOCUS       DATGFG010000649         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1592097, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000649 DATGFG010000000
VERSION     DATGFG010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592097"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..984
                     /locus_tag="VKQ52_12155"
     CDS             172..984
                     /locus_tag="VKQ52_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HLZ87994.1"
                     /translation="MNNEKERIITIIQTRRGSSRLPDKVLMPLQGKPLFVRQAERVRA
                     ARFSGRVIIATTTDAGDDPIEAVCRQEGLDCFRGDAADLLDRHYQAALRYPADAVIKI
                     PSDCPLIDPAIIDKVISFYLDHPGQYDFVSNLHPATWPDGNDVEIMTFPTLLRAWREA
                     DRPMEREHTTPYIWERPGEFRIGNVTMENGADHSMSHRFTIDYAEDYTFIRAVYDALY
                     PSNPLFGVDEILALLERRPDIYAINAGYAGVNWYRHHLDELKTVNAEQTKQL"
     gene            988..1869
                     /locus_tag="VKQ52_12160"
     CDS             988..1869
                     /locus_tag="VKQ52_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLZ87995.1"
                     /translation="MMTKTELDNLQSMALKVREHIIRMSEGGGCFIGASLSCVELMVY
                     CYAQVIRRERNHPQDESKKSGDNLGRDYLFLSKGHDVPALYGTFVEMGLLDAGRLGNH
                     LSTADSIYWHPNRQIPGVEFHSGSLGHLPAVAAGVALDCKLRLADNRIVVITGDGELN
                     EGSVWESILVANAYGLDNLTVVVDRNQFQANIRTEELVPLEPLADKFLAFGCTVSRVD
                     GHDFRELEKAFGEPAERGKVKVIIADTVRGKGLPSIEARADRWFCSFTAPEVAQLLEE
                     LHGHVKANIESETLVVR"
     gene            1880..2794
                     /locus_tag="VKQ52_12165"
     CDS             1880..2794
                     /locus_tag="VKQ52_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLZ87996.1"
                     /translation="MTYEELLIQTALEDERIIVMTAENRALVRNVPKAIGNRFIDTGI
                     TEQTMVGMAAGLALRGRIPVVHALAPFLTMRAYEFIRTDVGIAHLPVKLSGYIPGFLS
                     DGNGPTHQSIEDVSIMRGIPGMEVYCPADEDDLVTMLPHIWSSPAPAYTRVNHKKGTY
                     THAPYRPGKAEVIAEGTDVTLLVYGFLFENALRTRELLEKEGLSVGLVNMRSLKPVDE
                     EVIVKAAGFSRHLVTIEDHLETGGLYSIVAEVLLRRRMTASVTPLSLGERWFRPGLLG
                     QVLETEGFTPEKMVSTIISHINSVSYAK"
     gene            2784..>3576
                     /locus_tag="VKQ52_12170"
     CDS             2784..>3576
                     /locus_tag="VKQ52_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ87997.1"
                     /translation="MPNKVAFNEDYPVTTKSDELYKRAEPIMTPVTQTLAKGPGQYVD
                     GVAPKYLKKGKGSRVWDVDGNEFIDYNMAIGPLSLGYAYPRVDEAIVEQLKDGITFSM
                     MHELEVTLAELIHKVIPNAESIRISKTGADVTSAAVRVARAYTGRSKVLCCGYHGWHD
                     WYIGVTSRNAGIPEAVRDLSATFEYNDIASVREALDEDTACVILEPFVFEAPKNNFLQ
                     ELKALCEANGTLLIFDEMWTGFRIAVGGAQEYFGVTPDLACYSKAF"
BASE COUNT          772 a          872 c         1121 g          811 t
ORIGIN      
        1 aggtgcattc ctattttgat gtcgatgagt tgaggagatt ggcgcctgtt cagaacgggg
       61 gtatttcggc ggcgaccatt gcgggcgttt gccgccggta cattgaattt aaaggaacaa
      121 aagacgaatt tttaagaata accagaacgg ctacgactac aatcagctta catgaacaac
      181 gagaaagagc ggatcattac cattattcaa accagaaggg ggtcttcccg tctgcctgac
      241 aaggtgctga tgcctttgca gggcaagccg ctttttgtgc ggcaggccga acgggtaagg
      301 gcggcccggt tttccggccg ggtgatcatc gccactacga ccgatgcggg tgacgatccc
      361 atcgaagcgg tatgcaggca ggaagggttg gattgtttcc gtggcgatgc tgccgatctg
      421 ctggaccggc attaccaggc ggcgttgcgt tatcctgccg atgccgtgat caagatcccc
      481 agtgattgtc ccctgatcga ccccgcgatc atcgacaagg tcatttcttt ttatttagac
      541 catcccggac aatacgattt tgtaagtaac ctgcatccgg ccacctggcc cgacggcaat
      601 gatgtggaga tcatgacctt tccaacgctt ttgcgggcct ggcgtgaggc ggaccggccg
      661 atggagcggg agcataccac cccctatatc tgggagcgtc ccggggaatt ccgcatcggg
      721 aatgtgacga tggaaaatgg ggcggatcat tcgatgagtc accggtttac gatcgattac
      781 gcggaagatt atacctttat ccgcgctgtc tacgatgctt tatatccttc aaacccgctg
      841 ttcggggttg acgagatcct ggcgctgctg gagcggcggc cggatattta cgctatcaat
      901 gcgggatatg ccggggtcaa ctggtaccgg catcatctcg atgagctaaa gaccgttaat
      961 gccgaacaga caaaacaact ataagtaatg atgacaaaga ccgaattgga taacctgcaa
     1021 tcgatggccc tgaaagtaag ggagcatatc atccggatgt cggagggtgg cgggtgtttt
     1081 atcggcgctt cgctttcctg tgtcgaactg atggtctatt gttatgcgca ggtgatccgc
     1141 cgggagagga accatccgca ggacgagtcc aaaaaatcgg gcgacaattt agggcgggat
     1201 tacctttttc tttccaaagg gcatgatgta ccggccctgt atggaacatt tgtcgagatg
     1261 gggttactgg atgccggtcg cctgggcaat catttgtcca cggccgacag tatttactgg
     1321 catccgaacc ggcagatccc cggtgtcgag ttccattccg gttcgctcgg gcatcttccc
     1381 gcggtggctg ccggtgtcgc cctcgattgc aaactgcggc tggcggacaa ccggatcgtc
     1441 gtgatcacgg gtgatggcga gctcaacgag ggtagcgtat gggaatcgat cctggtggcg
     1501 aatgcctatg ggctggataa cctgacggtg gtggtagacc gcaatcagtt ccaggcgaat
     1561 atccggacgg aggaactggt tccgctggaa ccgctggccg ataaattttt ggcctttggc
     1621 tgtaccgtct cccgggtgga tggccatgac ttccgggagc tggaaaaggc ttttggcgag
     1681 ccggcggagc gcggcaaggt gaaggtgatc atcgccgata ccgtgcgggg caagggcctg
     1741 cccagcatcg aggcaagggc cgaccgctgg ttttgcagct tcaccgcccc ggaggttgcc
     1801 cagctgctgg aagagctgca tgggcatgta aaagcaaata ttgaatctga aacattggta
     1861 gtacggtaat aaagcgatca tgacatacga agaactcctt atacaaacag cattggaaga
     1921 cgagcggatc atcgtgatga cggcggagaa ccgggcgctg gtgcgcaatg tgccaaaggc
     1981 catcggcaac cggtttatcg atacgggtat tacggaacaa acgatggtgg gcatggccgc
     2041 ggggcttgcc ctgcgggggc gcattcccgt ggtgcatgcg cttgcgcctt ttctcactat
     2101 gcgggcctat gaattcatcc ggacggatgt aggcattgcc catctgccgg taaagctcag
     2161 cggctatatc ccgggttttc tttcggacgg caacggtcct acccatcagt ccatcgaaga
     2221 cgtttcgatc atgcggggta tccccgggat ggaggtgtat tgtcccgccg acgaggatga
     2281 ccttgttacc atgttgccgc acatctggtc ttcccctgcg ccggcctata cgcgggtcaa
     2341 ccataagaag ggcacgtata cccatgcgcc ctaccggccc ggaaaggccg aggtgatcgc
     2401 cgaaggaacg gatgtgaccc tgctggtgta tggattcctg ttcgagaatg cgctgcggac
     2461 gagggaactg ctggagaagg aagggctctc ggttgggctg gtgaatatgc ggtcgctaaa
     2521 acccgttgac gaggaggtga tcgtaaaagc ggccggtttc tcccggcacc tggtgaccat
     2581 cgaggaccac ctggagacgg gggggcttta ttccattgtg gcggaggtgt tgctgcggcg
     2641 gcggatgacg gcgtctgtga ccccgctttc gcttggggaa cgctggttcc ggcccgggct
     2701 gttggggcag gtactggaga ccgagggatt tacccctgaa aagatggttt cgacaataat
     2761 ttcccatata aattctgtta gttatgccaa ataaggttgc ttttaatgaa gattacccgg
     2821 taacgacgaa atccgatgag ctttataagc gggcggaacc gatcatgacg cctgtgaccc
     2881 agacccttgc caagggtcct ggtcaatatg tggatggtgt ggcgcccaaa tacctgaaga
     2941 aggggaaggg gtcgcgtgtg tgggatgtgg acggcaatga attcatcgat tataatatgg
     3001 cgatcggtcc gttgtccctg ggctatgcct atccgcgggt ggatgaggcg atagtcgaac
     3061 aactgaagga tgggattact ttttccatga tgcatgagct ggaggtaacg cttgccgagc
     3121 tgatccacaa ggtgatcccg aatgccgagt cgatccggat cagcaagacg ggagccgatg
     3181 tcacgagcgc cgcggtccgg gtggccaggg cgtataccgg caggagcaag gttttgtgtt
     3241 gcggctatca tggctggcac gactggtata tcggggttac gagcaggaat gcggggatac
     3301 ccgaagcagt cagggatctg agcgctacgt tcgaatataa tgatatcgcc tctgttcgcg
     3361 aggcgctgga tgaggatacc gcctgtgtca ttttggaacc ctttgtattc gaggcgccga
     3421 aaaataattt cctgcaggag ctgaaggctt tgtgcgaggc caacggcacc ctgctgatct
     3481 tcgatgagat gtggacgggt ttccgcatcg cggttggggg cgcccaggaa tattttgggg
     3541 tgacgcccga tctcgcctgt tattcgaagg cgtttg
//
LOCUS       DATGFG010000650         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_46389, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000650 DATGFG010000000
VERSION     DATGFG010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46389"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VKQ52_12175"
     CDS             <1..259
                     /locus_tag="VKQ52_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ87998.1"
                     /translation="PLIGKLAYQQYLAVAKRSLKLDSTIQGTERVSFQVDQKGQITNY
                     KIEQSVSPAHDAGVIQLISNGPAWKLLHGRKARAVVSLSFP"
     gene            324..1166
                     /locus_tag="VKQ52_12180"
     CDS             324..1166
                     /locus_tag="VKQ52_12180"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HLZ87999.1"
                     /translation="MSKLFVCLVLTACLYQNSYAQSIKTPPPSPPQSVKQDFGLSTIE
                     LSYSRPGMKGRKIFGDLVPYGKVWRTGANQATTLTFGDPVTIGGTKIPAGKYGLLSIP
                     GADEWTLIITHQLDVTNPAAYKPDQDIVRVQAKPFQLPFPMETFSILFANLTPSSCDL
                     QLIWENTVVVLPITTDVDTRVMAQIDNAMNKDIHPYFTAAFYYLENGKDLNKALEWFD
                     KAIAQDPSAGGSFFIVYQKARCLAKMGRKAEAKTTAQKGIELAKQANNADYVALNEKL
                     IATL"
     gene            complement(1228..>2595)
                     /locus_tag="VKQ52_12185"
     CDS             complement(1228..>2595)
                     /locus_tag="VKQ52_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88000.1"
                     /translation="LDKVKYGINLYKYDGRMKQLQQVGLQGSDKSFGPFPPRMTQFAG
                     KLLIFYYQVADNGAIQLLYSAVDPESLDISAGKLLYSISEKNVGLFKLEKAVFGNKLR
                     LCLSPDSSKLLVSQSGNTNEMFACTINADFSIEKPVVSKVKANMEDFVVESALIDGVG
                     NRYFTYSFFEDKAKKNGVFVQNSSGKEGFLSFRANPDGGEPGKLSLQLSKDYGKAFVY
                     GASSTEQLEEGVVLVTVDADGIKLGKPQLCSFPAELKDRLRKMDYTVKRHDPEVEKGV
                     SYKSAILEDGTVVLTGFPEDHENHAPMISPGQHFASSISRDYAGPIFNIFIKDGKSSL
                     GVIYRNQRLSPASIPIAIPYGNKLICIYTDSEKGIASDDPHTNDKVKDESDLVLAMAI
                     LNSDGTIISKKKLAAPVGSANFFTMYMQQLNSRQFLIPLGRERVNLARYYSELEEWAT
                     LDINP"
BASE COUNT          648 a          771 c          561 g          615 t
ORIGIN      
        1 ccccctgatc ggcaaactcg cctatcagca atatctcgct gtcgccaaac gatcgcttaa
       61 gctggactcc accatccagg gtaccgaacg ggtttcgttc caggtagatc aaaaagggca
      121 aataacgaat tacaaaatag aacaatccgt ttcccctgcc catgacgccg gcgtcatcca
      181 gctgatctcg aacggaccgg cctggaaatt actacatggc agaaaggcca gggcggtagt
      241 aagccttagc ttcccctgac cggctttaat tggatggtct ccgatttgta ccgtacattt
      301 agctcctcaa aaaccaccac tccatgagca agttattcgt ttgtcttgtc cttacagcct
      361 gcctgtacca gaactcctat gcacagtcta ttaaaacacc accgccttcg ccaccgcagt
      421 ccgtaaagca ggacttcggc ctttcgacga tagagctgtc ctattcccgc ccgggaatga
      481 agggccgcaa gatctttggc gacctcgtac cctacggcaa agtctggcgc accggcgcaa
      541 accaggccac gacactcacc ttcggcgacc cggtgaccat cggcggcaca aaaattcccg
      601 cgggtaaata cggcctgctc agtattcccg gcgccgacga gtggaccctc atcatcactc
      661 atcagctgga tgtcacgaac ccggccgcct acaaacccga tcaggatatc gtccgggtac
      721 aggccaagcc cttccagctg cccttcccca tggagacctt ttccatcctc ttcgccaacc
      781 tcacgcccag ctcctgcgac ctgcagctca tctgggagaa caccgtcgtc gtactgccca
      841 taacaaccga cgtggacacc agggtcatgg cacagatcga caatgccatg aacaaggaca
      901 ttcatcctta ttttaccgct gccttctact atttggagaa cgggaaagac ctgaacaagg
      961 ccctggaatg gtttgacaag gccatcgcac aggacccatc cgccggcggt tccttcttca
     1021 tcgtctatca aaaggcccgc tgtcttgcca aaatggggag aaaagccgaa gccaaaacca
     1081 ccgcgcaaaa aggcatcgag ctcgccaaac aagccaataa tgccgactac gtcgcgctga
     1141 acgagaaact catcgccacg ctctagcaat aaaaaaaccc ggctaagcgt cgggtttttt
     1201 actttgaata cttcggtgag gcgcccacta cgggttgata tccaacgttg cccattcctc
     1261 cagctctgaa taatatcggg ccaggttcac cctttcccgc ccaaggggaa tcagaaactg
     1321 gcgtgaattc agctgctgca tgtacatcgt aaaaaagttc gcgcttccta ccggggcagc
     1381 cagcttcttt ttgctgatga tcgttccatc actgttcagt atagccatcg ccagaacgag
     1441 gtcgctttcg tccttgacct tgtcattcgt atgaggatcg tcagatgcaa tccctttttc
     1501 gctgtcagta taaatacaga tcaatttatt gccgtacgga attgctatcg gtatggacgc
     1561 aggagataac ctctggtttc ggtaaataac gccaagactg gactttccat cctttataaa
     1621 tatattgaat ataggtccgg cataatctct tgatatcgag gacgcgaaat gctgtccggg
     1681 agatatcata ggcgcgtggt tctcatgatc ctctggaaat cccgtcagta ctacggtacc
     1741 atcttccagt atagcgctct tatagctcac ccctttctct acctcagggt catgacgttt
     1801 cactgtataa tccattttac gcagccggtc tttcaactcc gccggaaacg aacatagctg
     1861 cggcttccca agctttatcc cgtcggcgtc aaccgtcacc aggacaaccc cctcctccag
     1921 ctgctcggtt gaactggccc cgtaaacaaa tgctttccca taatcctttg atagttgtaa
     1981 cgaaagcttg cccggttccc cgccatcggg gttcgcgcga aaagacagaa aaccctcctt
     2041 gccactggag ttctgcacga acacgccatt ctttttcgcc ttatcctcaa aaaaggaata
     2101 ggtaaaatat cggttaccca caccatcgat caaggcactt tctacgacaa agtcttccat
     2161 attcgccttg accttcgaca ctaccggttt ttcaatggaa aagtctgcgt tgatcgtgca
     2221 ggcgaacatt tcgttggtgt tgcctgactg gcttaccagt aatttactgc tgtccgggct
     2281 caggcaaaga cgcagcttat ttccaaagac cgccttctcg agtttaaata accccacatt
     2341 cttttcagaa atggaataga gcagcttccc ggctgaaata tccagggact cgggatctac
     2401 cgccgaataa agcaattgaa tggcgccatt gtccgccacc tggtaataaa agatcagaag
     2461 ctttcctgca aactgggtca tcctgggcgg aaagggcccg aacgatttat cgcttccttg
     2521 cagccccacc tgctgcaact gcttcatacg gccgtcatat ttataaagat tgatcccata
     2581 cttgacctta tccag
//
LOCUS       DATGFG010000651         4606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_867708, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000651 DATGFG010000000
VERSION     DATGFG010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867708"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="VKQ52_12190"
     CDS             <1..1920
                     /locus_tag="VKQ52_12190"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ88001.1"
                     /translation="STDVYGSYFFNNQHTVVRQTDSAISPVQTNYPSDSALVSNGNSY
                     NISRIQNHRIYLNVESRFDTNNSLIFRPNITFQRSTPSGTSFTEETDNHLNPINSSYN
                     LTSSTNTGFNINSTNLQFRHRFAKPYRTISIDVNTTANINNGTGTNYAINNIFVKHIT
                     DTLNQFYYDSLHSWTVTPTLSYTEPIAKNQILQLTYNHTYVRSTTINRTYDYVDSLKA
                     YTRFDYLFSNSYKFTQNSDQVALAWRFQNAKYNLSVGSGIQWTDFNSDNTTKGITVAH
                     NYVNYTPTVNFNYQFSNTQHLRLNYMGRTGTPSASQLQPLTTTSDSLSFQMGNPALKP
                     QFTHSLRILYANFDPGTQRVIFATVNASTIVNDIQSAVYRNSLGGQNSTYVNLNGTYN
                     ISGFFNYGFPLKHPKSNLNFMTNVTYAQSQSLLAQDSLSAADNRYAHEYTRNTGLNET
                     IKWTTNIRKNFDMNFSSTTGYTINKNPGVQRSKSDSSKTGSVSSSTNLNTFTETLSAE
                     FTAYTNSGWLIAASFDYTYTYTHSNTYTAHIPLLTPSIAKQLFKKKNGELRLTVFDVL
                     NKNTSVSKTVGSYGAVTYNRTNVLSRYAMLTFTYNLNNFAGANQRRMPGMFPGRFRGG
                     GGGGFRDGGGNFPIN"
     gene            complement(1917..2087)
                     /locus_tag="VKQ52_12195"
     CDS             complement(1917..2087)
                     /locus_tag="VKQ52_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88002.1"
                     /translation="MKQDHLSYNVRKARIYKDIYRPSFKLSYFLGTLLSVIIHGQSRV
                     GSPSMHEGRRRY"
     gene            2240..2809
                     /locus_tag="VKQ52_12200"
     CDS             2240..2809
                     /locus_tag="VKQ52_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88003.1"
                     /translation="MRSAILALAPVMLSVSLSAQGVFSNKTQTIVEKVIQDYPNHFYN
                     IKGELIGHATQTSSYKSTLELPGASSSTITLVTASGAGGSGWTCTVLQTASFEEARAR
                     FSEIYGQLSNSIVSAAGQKTFILSGQYEAPTADKKDNEVIFTLLPGVGEMKKLRVELM
                     LCEEGQDWVLSLTVNDDPQEMRQLAANGR"
     gene            complement(2820..>4606)
                     /locus_tag="VKQ52_12205"
     CDS             complement(2820..>4606)
                     /locus_tag="VKQ52_12205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ88004.1"
                     /translation="RAGVLSSVALPIFLKDGSFWGFVGFDEVKEEREWYEAEFAILRS
                     YASSLAAAIERKQIEVELVQAKEMAESASHAKSEFMANMSHELRTPMNGIIGFTDLVL
                     TTELQKAQRDYLQNVKKSAYGLLEIINDILDFSKIEAGKLLIDHLPFKLDELIEETID
                     MLTLKAFEKKLEMLYRVDPSIPSQFLGDPVRIRQIVVNLLGNAIKFTGAGEILVSIRK
                     SSNIYQSENKSFIRLAIEVKDTGIGIRREKLQKIFESFTQADSSTTRKYGGTGLGLTI
                     SRSLAELMGGYLTVESEPAKGSSFTLHLTLEIANQQPEIQQPQKALLQKVLLVDDNAT
                     NLQLMQEIFSYFHIESVLASGAEEAIARMKEARQQKDPFSLVITDHLMPGMDGIALVK
                     ELKRLFPGNNAPYVLMLSSLEKDLYQHEAAKAGITKFLSKPVKMHELYGALLSLFEMN
                     RQQDGQHFQAPGSSVEKISEQPASIMVADDDPINMLLISEVLRRMGFEVIQVSNGKEA
                     LERLPHCDPVIIFMDVNMPEMDGYTTTRHIRLMPEPWCRLPIIALTADAMKGDREKCL
                     EAGMNKYISKPFRLEEIDEVLRNYTLMV"
BASE COUNT         1039 a         1245 c         1270 g         1052 t
ORIGIN      
        1 tcgactgatg tctatggcag ctattttttc aacaaccagc ataccgtggt gaggcagacg
       61 gatagcgcca tctcgccggt acagaccaat tacccatccg actcggccct ggtatccaac
      121 ggcaattcct acaatatctc ccggatccag aaccaccgga tctacctcaa cgtggaaagc
      181 cggttcgaca ccaataactc cctgatcttc cggcccaata tcaccttcca gaggagtaca
      241 ccgagcggga cctcgttcac ggaggagacc gataaccatc ttaacccgat caatagttcc
      301 tataacctta cctccagcac caataccggc tttaatatca acagcaccaa cctgcaattc
      361 cggcaccggt tcgccaagcc ctaccggacg atctcgatcg atgtcaacac cacggccaat
      421 atcaataacg gcaccgggac caattacgcg atcaacaata tttttgtaaa gcatatcacc
      481 gatacgctga accagtttta ttacgactcc ctgcatagct ggacagtcac gcctaccctg
      541 tcttatacgg aaccgatcgc gaagaaccag atcctgcagt tgacctataa tcatacctat
      601 gtccggagca cgacgatcaa ccggacctat gattatgtgg attcgttgaa ggcctatacc
      661 cgtttcgact acctgttcag caactcgtat aagttcacgc agaattcgga ccaggtggcc
      721 ctggcctggc gcttccagaa tgccaagtat aacctgtcgg tcgggtccgg catccagtgg
      781 accgatttca acagtgacaa cacgacgaag gggatcaccg tggcgcacaa ttatgtgaat
      841 tacacgccga cggtcaactt caattaccag ttctccaata cccagcattt gcggctcaac
      901 tatatgggaa ggacgggaac acccagtgca tcgcagctgc aaccgctgac cacgacgtcg
      961 gacagtctca gtttccagat ggggaaccct gcgctgaagc cgcagttcac ccattcgctg
     1021 cggatcctgt atgctaattt cgatcccggc acccagcggg ttattttcgc tacggtgaac
     1081 gccagcacca tcgtcaacga tatccagtcg gcggtgtacc gcaacagcct tggcgggcag
     1141 aacagcacgt atgtcaacct gaacgggaca tataatatca gcgggttctt caactatggt
     1201 ttcccgctga agcatccgaa gtcgaacctg aacttcatga ccaatgtcac ctatgcgcag
     1261 agccagagcc tgctggcgca ggactcgctg tcggcggcag acaaccggta tgcgcatgaa
     1321 tacaccagga atacggggct gaacgagacg atcaagtgga cgacgaatat caggaagaac
     1381 ttcgacatga acttcagttc caccacgggg tatacgatca ataagaatcc gggggttcag
     1441 cgctccaaga gcgattcctc gaagaccggg agtgtcagca gcagcaccaa tctcaacacg
     1501 ttcacggaga cgctctcggc ggagtttacg gcttatacca acagcgggtg gctcatcgcg
     1561 gcgagtttcg actacaccta tacatatacg cactccaata cctatacggc gcatattccc
     1621 ctgctgaccc ccagcattgc caagcaattg ttcaaaaaga agaacgggga actccggctg
     1681 accgtattcg atgtgctgaa taagaataca tcggtgtcca agacggttgg cagctatggg
     1741 gcggtgacgt ataaccggac gaatgtgctg agccgttacg cgatgcttac cttcacttat
     1801 aacctcaaca atttcgccgg ggcgaaccag cgacggatgc cggggatgtt cccgggccgg
     1861 ttccgcgggg gcgggggcgg cgggttccgg gacggcggcg gcaatttccc gatcaattaa
     1921 tagcggcggc gtccttcatg catgctcgga cttcccaccc gggactgacc gtggatgatg
     1981 acggagagca gcgttccaag aaagtaggaa agtttgaaag aaggtctata gatgtccttg
     2041 taaatgcggg ctttacgaac gttgtaggag aggtgatctt gcttcatcga tattggattt
     2101 aaggatggaa accttttgtg gatacaggat tttaacgatc aatttacggg tttattgtat
     2161 aaagttgtgt taaagcgggg cgtagatttg ctattgcagg ggggagtaat cggttgtatc
     2221 ttgccgaaaa tccttactta tgagaagcgc aatactcgcg ttagcgccgg tgatgctctc
     2281 cgtttccctc tctgcccagg gggtcttttc aaacaagacc cagaccatcg tagaaaaggt
     2341 catccaggat tatcccaatc atttttacaa catcaagggg gaattgatag gtcacgcgac
     2401 gcagacctca agttacaagt ccacactgga gttgccgggg gcatcttcca gcacgatcac
     2461 gcttgtcacc gcctccgggg ccggtgggtc cggctggacc tgcaccgtgc tgcaaacggc
     2521 atccttcgag gaggccaggg cccgtttttc cgagatctat ggtcagctca gcaatagcat
     2581 cgtctccgcc gcgggtcaaa agacctttat cctgagcggt caatatgaag cacccacagc
     2641 tgataaaaaa gataatgaag tgatctttac gcttctgccc ggtgtcgggg aaatgaaaaa
     2701 gctccgggtg gagcttatgc tgtgtgaaga aggccaggac tgggtccttt cgctcacggt
     2761 gaacgacgat ccccaggaga tgcggcaatt ggctgccaat gggcgatgac cggcccgaac
     2821 tacaccatca gggtataatt ccgtaagact tcgtcgatct cttccagccg gaacggcttg
     2881 gagatgtatt tgttcatgcc ggcttcgagg catttttccc ggtcgccctt catggcgtcc
     2941 gcggtgaggg cgatgatcgg cagccggcac cagggttcgg gcatcagccg gatatgccgg
     3001 gtagtggtat aaccatccat ctcgggcata ttcacgtcca taaagatgat gaccgggtcg
     3061 cagtggggca accgctccag tgcttccttt ccattgctga cctggatgac ctcgaagccc
     3121 atgcggcgca ggacctcgga gatcagcagc atattgatcg gatcgtcatc ggccaccatg
     3181 atgctggcgg gttgttcgga gatcttctcc acgctgctgc cgggcgcctg gaagtgctgg
     3241 ccatcctgtt ggcggttcat ctcgaacagg gacagcaggg cgccatacag ctcgtgcatt
     3301 ttcaccggtt tggaaaggaa tttagtgata cccgcctttg ccgcctcgtg ctggtagagg
     3361 tctttctcca acgaggagag catcaggaca tagggagcat tgtttcccgg aaagagccgt
     3421 ttcaattctt ttaccagggc gatgccatcc atgcccggca tcaggtggtc ggtgatcacc
     3481 aggctgaacg ggtctttctg ctgccgggct tccttcatcc gggcaatggc ctcttcggct
     3541 ccggaggcca gaacggattc gatgtggaag tagctgaaga tctcctgcat caactgcagg
     3601 ttggtggcgt tgtcgtcgac gagcagcact ttttgcagca gggccttttg cggctgctgg
     3661 atctccggtt gctggttggc gatctccagg gtcaggtgga gggtgaagga gcttcccttt
     3721 gccggttcgc tttcgacggt gagatagccg cccatcagct ctgccaggct gcgggagatg
     3781 gtgagtccca gcccggtgcc gccatattta cgggttgtgg agctgtctgc ctgggtgaag
     3841 ctttcgaaga tcttttgcaa cttttcgcgg cggataccga tgcccgtatc ctttacctcg
     3901 atcgccaggc ggataaagga tttgttctcg gactggtaga tattgctgga cttccggatg
     3961 gagacgagga tctcgcctgc cccggtgaat ttgatggcgt tgccaaggag gtttaccacg
     4021 atctgccgga tgcgcacggg gtcgcccagg aactgggagg ggatggaagg gtcaacgcgg
     4081 tagagcattt ccagcttttt ctcgaaggcc ttcagggtaa gcatgtcgat ggtctcctcg
     4141 atcagttcat ccagtttgaa ggggaggtga tcgatgagca gtttccccgc ctcgatcttg
     4201 gagaagtcga ggatatcgtt gatgatctcc agcaggccat aggcggattt ctttacattc
     4261 tggaggtagt cgcgttgcgc tttctggagt tcggtcgtga gcacgaggtc ggtgaagccg
     4321 atgatgccgt tcatgggggt acgtaattcg tggctcatat tggccatgaa ttcgctcttg
     4381 gcgtggctgg cggattcggc catttccttg gcctgtacca gttccacctc gatctgtttc
     4441 cgttcgatgg cggcggccag cgatgaggca taggaacgaa ggatcgcgaa ttcggcctcg
     4501 taccattccc gctcctcctt cacctcgtcg aatcctacga agccccagaa gcttccgtcc
     4561 ttcaggaaaa tgggcagggc gacactggac agtacgccgg cccgct
//
LOCUS       DATGFG010000652         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_481249, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000652 DATGFG010000000
VERSION     DATGFG010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481249"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VKQ52_12210"
     CDS             <1..263
                     /locus_tag="VKQ52_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88005.1"
                     /translation="YLISDRFLESGSYLRLDNATLGYTIKPHTQNVKSIRVYATGSNL
                     FVITKYRGTDPEINIGGLTPGIDNQNFYPKTRSYIIGVSANF"
     gene            283..1881
                     /locus_tag="VKQ52_12215"
     CDS             283..1881
                     /locus_tag="VKQ52_12215"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88006.1"
                     /translation="MKRTHILVFVLAALLGFGACTKLNVPVESQYVSGNFPTTTNDYA
                     ALFGTMYSNLSSNFAITYWRMQELSTDEAILPARDGNFDDGGQYRQMHYHTWTYDHPY
                     VTGIWQWGFGGINNCNRLITLTIQSKADTVTKTANLAEIRAMRALYYFFMMDLYGNVP
                     LITTFPVATPPSTQPRAKVFQFIETELLSIAQQLPSKTSSLATNTLQYGRPTKAMAFA
                     VLMKMYLNADIYTGGATRYNDVVAMADSIQHNSNYYLDARFRDIFLPNNGPQINETIF
                     AIPYDQQIPGNQFTRFGFYYYLVNAYGFNVGESIAMSTTPEFYNRFNIPGDFRTKTWL
                     VGPQFYPDGTGGFTNQPVYYPGTTTQIVINPTLTLVAPKPMDLGNTIASQAEGIRSIK
                     YYPDPTTIQATRLNGNDVPVFRLADVYLMKAEAILRGATPTTVNGVLQTPGYLVNLLR
                     TRAGAQLAPTVTLDTLLDERARELSWEGWRRNDLIRFGLFEKSYPLPPSDPLTMNTDV
                     TRRLFPVPISEIKLNSNLAQNPGY"
BASE COUNT          478 a          559 c          513 g          459 t
ORIGIN      
        1 cctatctgat ctccgaccgt ttcctggaaa gcggctctta tctccgcctg gataatgcga
       61 ccctggggta tactatcaaa cctcatacgc agaatgtgaa aagcatccgt gtctacgcaa
      121 cgggcagtaa ccttttcgtt atcacgaaat accgcggcac agaccccgag atcaatatcg
      181 gcggtttgac gccgggtatc gacaatcaga atttttaccc caaaacacgt agctatatca
      241 tcggtgtaag cgccaacttc taatcggcaa aactctaaaa aaatgaaaag aacacatata
      301 cttgtttttg tactggcagc tttgctcggt ttcggcgcct gtaccaaact gaatgtaccg
      361 gtcgagtccc aatatgtaag cggcaatttt ccgacgacta ccaatgacta tgccgccctg
      421 ttcggcacca tgtacagcaa cctttcatcc aacttcgcga tcacctactg gcggatgcag
      481 gagctatcca cggacgaagc catccttccg gcgcgcgatg gtaatttcga cgacggaggg
      541 cagtaccggc agatgcacta tcatacctgg acctatgatc acccctatgt caccggcata
      601 tggcagtggg ggttcggcgg catcaataac tgtaaccggc tgatcacgct gacgatccaa
      661 tcaaaagccg atacggtcac caagacggcc aatcttgccg agatcagggc catgcgcgcc
      721 ttgtattatt ttttcatgat ggacctctac ggcaatgtgc cgctgatcac gaccttcccg
      781 gtagccacgc cgccttctac ccagccgagg gcaaaggtct tccaattcat cgaaacggag
      841 ttattgagca tcgcgcagca actgccttcc aagacgagca gcctggccac caataccctg
      901 caatacggac gtccgaccaa ggcgatggcc tttgccgtgc tgatgaagat gtacctgaat
      961 gcggacatct acacaggcgg cgccacccgg tataatgacg ttgtcgcgat ggccgacagc
     1021 atccagcata acagcaatta ttacctggac gcccggttcc gggatatctt tttgcccaat
     1081 aacggtcccc agataaacga aaccattttt gcgatccctt atgaccagca gatccccggc
     1141 aatcagttta cccggtttgg attttactat tatctggtga atgcctatgg ctttaatgta
     1201 ggtgagagta ttgctatgag caccacgccc gagttctaca accggtttaa tatcccgggt
     1261 gatttcagga cgaagacatg gctggtgggt ccgcaattct atcctgatgg cacgggaggg
     1321 tttacgaacc agccggttta ttatcccggc accacgacgc agatcgtcat caaccctacc
     1381 ctgacgctcg tggcgcccaa gcccatggac ctggggaata ccattgcgag ccaggcggaa
     1441 gggatcaggt cgataaagta ttatcccgac cctacgacca tccaggctac gcggctcaac
     1501 ggcaatgacg ttccggtgtt ccggcttgcc gatgtctacc tgatgaaggc ggaagctatt
     1561 ttgcgtggcg ccactcccac tacggtgaac ggcgttctgc agacacccgg ctatcttgtc
     1621 aatttgctgc gtacgcgggc gggtgctcag cttgcgccca ctgttaccct ggacaccctg
     1681 cttgacgaac gtgcccggga gttgtcctgg gaaggctggc ggcgtaatga ccttatccgg
     1741 tttgggctgt ttgaaaagtc ttacccgctg ccccccagtg acccgctgac gatgaatacg
     1801 gatgtgacca ggagactttt ccctgtgccg atctcggaga ttaaattgaa tagtaacctg
     1861 gctcaaaacc ccgggtattg agattgtgat aatgatataa aaaaaagggc gccttcgggc
     1921 gccttttttg ttcaatagaa tccggcgtcg gagaggtgct cgtcgataag gaaaacggtg
     1981 aggctgcggg ggccgtgggc gccgaggac
//
LOCUS       DATGFG010000653         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_960190, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000653 DATGFG010000000
VERSION     DATGFG010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960190"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2296
                     /gene="priA"
                     /locus_tag="VKQ52_12220"
     CDS             <1..2296
                     /gene="priA"
                     /locus_tag="VKQ52_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HLZ88007.1"
                     /translation="ETILVFNEEAGDDLSHLDNDEYIVAEALQIKKELKLADVQQVLD
                     SSHVYPVIKRLIEKKICFVWESLKETYTPKKENYVVLNAEYNDEDRLADLLNNWSRAP
                     KQQELLLAYLHISRTEGDVTRAELLKKSGASDAQLKGLVDKQILRVEQRVVDRIHYLP
                     RDIKIDFELTPAQQQAFEQVNTVFGQKAVCLLHGVTSSGKTLVYIRQMEQVIRRGQQV
                     LYLLPEIALTAQIIRRLKEHFGGYIGIYHSKFSQNERLEIWNKIRTGELKIILGARSA
                     LFLPFNNLGLVVTDEEHDPSYKQQEPAPRYNARDAAIYYASLFGAKVLLGSATPSVET
                     YYNATTGKYGLVEMLERYGLGRLPEIQIVDTKALRFARTPVKKEGEAFAGASWLTDIE
                     GGAGPGRSERGAGPGGMGREERDAAREKEVRDPATGARDNGKPILSPALHAAIERALG
                     AGRQVILFQNRRGYSPYQVCEVCGWIPQCRNCDVSLNFHKLTNKLHCHYCGTVYPPVQ
                     TCAACGNHKFVQRNFGTERIEEYLEEVFPKARVARMDIDSIRGKTAHDTLIQQFEQGR
                     IDILVGTQMVVKGLDFENVSVVGILDADSILSFADFRVNERSFQLMEQVSGRAGRKDA
                     DGLVLIQVANTAHPVLGYVQAHDYALFYRHEIAARQQFGYPPFSRVIRVTFRHRDKEV
                     VQSAAQFFANHMKKDFGSWMVGPAEPMVGRVRNQYLMELMLKLPKDGHTIAFAKQVIQ
                     QQTAIVQNARQWRSVVIIPDVDTI"
BASE COUNT          510 a          578 c          799 g          481 t
ORIGIN      
        1 cgagaccatc cttgttttca acgaggaggc gggggatgat ctctcccatc tcgacaatga
       61 tgaatatatc gtggccgagg ccctgcagat caagaaagag ctgaagctgg ccgatgtgca
      121 gcaggtgctc gactcttccc atgtctatcc cgtgatcaaa cggctgatcg aaaaaaagat
      181 ctgttttgtc tgggaatcgt taaaagagac ctacaccccg aagaaagaga attatgtggt
      241 cctgaatgcg gaatacaacg atgaggaccg gctggccgac ctgctcaaca actggagccg
      301 cgcgccgaag caacaggagc tgttgctggc ctatcttcat atttcccgga cggagggcga
      361 tgtgacgcgg gcggagttac tgaagaaatc cggcgcttcc gacgcccagc tcaaggggct
      421 tgtcgacaag cagatcctgc gggtcgaaca gcgggttgtc gaccggatcc attacctgcc
      481 ccgggatatc aagatcgatt tcgagctgac accggcgcag cagcaggcct tcgagcaggt
      541 gaacaccgtc tttggccaga aggcggtttg ccttttgcat ggcgtgacat cgagcgggaa
      601 gaccctggtg tatatccgtc agatggagca ggtgatccgc cggggtcagc aggtgttgta
      661 cctgctgccg gagatcgcgc taacggcgca gatcatccgc cggctgaagg agcattttgg
      721 cggctatatc gggatctatc attccaagtt cagccagaac gagcggctgg agatctggaa
      781 caagatccgg accggggagc tgaagatcat cctcggggcg cggtctgcgc tttttcttcc
      841 ttttaacaac ctcgggcttg tcgtcaccga tgaggaacat gacccgtcct ataaacagca
      901 ggagccggcc ccgcggtata atgcgcggga tgcagccatc tactatgcgt cgctttttgg
      961 agcgaaggta ttgctgggca gcgccacccc gtcggtggag acctattaca acgcgacgac
     1021 ggggaagtat gggttggtgg agatgctgga gcgatatggg ctggggcggc tgccggagat
     1081 acagatcgtc gatacgaagg cattgcggtt tgcccggacg ccggtgaaga aggagggaga
     1141 ggcttttgcg ggtgcatcct ggttgacgga tatagaaggg ggtgcgggac ccgggagatc
     1201 ggagcgaggc gcgggacccg ggggaatggg ccgggaggag cgggatgccg ccagggagaa
     1261 ggaagtgcgc gatccggcga cgggggcgag ggataacggc aaacccattc tcagcccggc
     1321 cctgcatgcg gcgatagagc gggcgctggg ggcgggccgg caggtgatcc tcttccagaa
     1381 ccggcggggg tattcgccgt accaggtgtg tgaggtatgc gggtggatcc cgcaatgccg
     1441 gaactgtgat gtatcgctga actttcataa gctgaccaat aagctgcatt gtcattattg
     1501 tgggacggtg tatccgcccg tgcaaacctg cgcggcctgt ggcaaccaca agtttgtcca
     1561 gcgcaatttc gggacggagc ggatcgagga atacctcgag gaagtattcc ccaaggcccg
     1621 ggtggcgcgg atggatatcg acagtatccg gggtaagacc gcgcatgaca cgctgatcca
     1681 gcaattcgag cagggaagga tcgatattct tgtcggtacg cagatggtgg tgaaggggct
     1741 ggatttcgag aatgtgagtg tagtggggat cctcgatgcg gacagtatcc tgagttttgc
     1801 cgatttccgg gtcaatgagc ggagttttca gctgatggag caggtaagcg gccgggcggg
     1861 gcggaaggat gcggatgggc ttgtcctgat ccaggtggcg aatacggcgc atcccgtgct
     1921 ggggtatgtg caggcgcatg actatgcgct tttttaccgg catgagatcg ccgcgcggca
     1981 gcaatttggt tacccgcctt tttcgcgggt gatccgggtg acgttccgcc accgggacaa
     2041 ggaggtggta cagtcggcgg cgcagttctt cgccaaccat atgaaaaagg attttggctc
     2101 ctggatggta gggccggccg agccgatggt ggggcgggtc cgcaatcaat acctgatgga
     2161 gctgatgctg aagctgccca aggacgggca taccatcgct tttgccaagc aggtgatcca
     2221 gcagcaaacc gccatcgtgc agaatgcgcg gcagtggcgg agcgtggtga tcatcccgga
     2281 tgtggatacg atataatttt accgttatga taaatattgc cgggaatgtc ccgctgaaga
     2341 agtacaatac gtttggcatc gatgtgtc
//
LOCUS       DATGFG010000654         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_101246, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000654 DATGFG010000000
VERSION     DATGFG010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101246"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VKQ52_12225"
     CDS             <1..308
                     /locus_tag="VKQ52_12225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88008.1"
                     /translation="IEQVLINLVVNAIEAVKDRDQPLITLSGNAAKDGRPVIKVADNG
                     MGMSPEVMENIFIPFFSTKKNGSGIGLSLCKQILLLHKGNIQVQSVEGVGTAFYLHF"
     gene            complement(330..1103)
                     /locus_tag="VKQ52_12230"
     CDS             complement(330..1103)
                     /locus_tag="VKQ52_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ88009.1"
                     /translation="MKVLIVEDERPASENLVEELQAIDDNIDVVAGCNSVDETVRWLS
                     NNPQPDLILMDIQLSDGLSFNIFKACEVTCPVIFTTAYDKYLTQAFEYSSIDYLLKPI
                     SQDRLKNAIRKYKNLRNHFVNAQDALANGQALADYSSHPDRVRSRILVRKGVEFQAVR
                     VEDAGYFFTEHKLIFLVDKENRKYMAEKSNLSELEEELDRNIFYRANRKYIINANYIK
                     RFKPLERSKISVELVLPVNEEIIISQENSASFKKWIGES"
     gene            complement(1273..2307)
                     /locus_tag="VKQ52_12235"
     CDS             complement(1273..2307)
                     /locus_tag="VKQ52_12235"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ88010.1"
                     /translation="MNKQIVLNDRLVKSIGIPVLGLFIPNLSGLVTNRLYSYSELAAC
                     YLFFIVVAFLVWEGNVRLMYYIREKFPWSKKSYYKIIVALFFANIVYSGAISLLLLRL
                     WQFCSREPQGDNEHLVNTVLIIIIAACFITNIYEILFLNLEKEYNESRVEQLNVAKAQ
                     AELEALKNQIDPHFIFNSLNTLSFLITRDPKNARLYNDTLARVYRYILSNKERDLVLL
                     REEVEFISNYFYLLKIRFADAISMVIEITDLSAENYLIPPISLQALVENAIKHNEFSE
                     KKPLAIDVSISSDYVIVRNVMNRRSNQQPTSKIGLSNLDNRYKLITKRNIQVENNFES
                     FIVKLPIVRF"
     gene            3192..>3371
                     /locus_tag="VKQ52_12240"
     CDS             3192..>3371
                     /locus_tag="VKQ52_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88011.1"
                     /translation="MRKLVLFLVASLLVSVLVKAQTREVTGKVTDSAGIPLAGASINV
                     RGSRVGTSAGPDGSFR"
BASE COUNT          704 a          686 c          993 g          988 t
ORIGIN      
        1 tgattgagca ggtgctgatc aatttggtgg tcaatgcgat cgaggcggta aaggatcggg
       61 atcaacccct gatcaccctg tcgggtaatg cggccaagga cggacgcccg gtaatcaaag
      121 tcgcagacaa tggcatgggt atgtcgccgg aagtgatgga gaatattttc atccccttct
      181 ttagcaccaa gaaaaatggg agcggtatcg gcttgagcct ttgcaaacaa atcctccttt
      241 tgcataaagg gaacatccag gtgcagtcgg ttgaaggcgt cggtacggca ttctatttgc
      301 atttctgacg gcgccttcgc gtcggaaggt tagctttcac cgatccattt tttgaaggag
      361 gccgagttct cctggctgat gatgatctct tcgttgacgg ggaggacgag ttccacgctg
      421 atcttgcttc gttcgagggg tttgaaccgt ttgatatagt ttgcgttgat gatatacttg
      481 cggttggcgc ggtagaagat gttcctgtcg agttcttctt cgagttcgct gaggttgctt
      541 ttctccgcca tgtatttccg gttctctttg tctaccaaaa agatcagctt atgttccgtg
      601 aagaagtagc cggcgtcctc gacgcggacg gcctggaatt ccacgccttt tctgacgagg
      661 atgcgggatc tgacccgatc gggatgggag ctgtagtcgg ccagggcctg gccgttggcg
      721 agggcatcct gggcgttgac gaaatggttg cggaggttct tgtattttct tatggcgttt
      781 ttcagtcgat cctggctaat aggttttagc agataatcga tgctgctgta ttcgaaggcc
      841 tgggtgaggt atttgtcata ggctgtggtg aagatgacgg ggcaggtgac ctcacaggcc
      901 ttgaagatgt tgaaggagag tccgtcggag agttggatat ccatcaggat gagatcgggt
      961 tggggattat tgctcagcca tctgaccgtc tcgtcgacgc tgttacagcc tgccaccaca
     1021 tctatattat cgtctatggc ctgtaattcc tcgaccaggt tctcggacgc gggccgttcg
     1081 tcttctacga taaggacttt catgtggcta gtttttgttt ttcaaaatta gttagtattc
     1141 ccttgctttc attaccgttg gtgtaaagaa gcctccttcg atgcggctag tatatgacgt
     1201 agtcatacag tgaaatgttg cacatgggac ggaataaaaa tgggacggcg gtgcggattt
     1261 cttcgggcgg cttcagaacc tgacgatcgg cagttttaca atgaaggatt cgaaattgtt
     1321 ttcgacctgg atatttcgtt ttgtgatgag tttatagcga ttgtcgaggt tgctgaggcc
     1381 tatcttggac gtgggttgct ggttgctgcg gcggttcatc acgtttcgga cgatgacata
     1441 atcggacgag atggagacgt cgatggccag tggttttttc tcgctgaatt cgttatgttt
     1501 tatggcgttt tcgacgaggg cctgcaggga tatcgggggg atgaggtagt tttcggcgga
     1561 caggtcggtg atctcgatga ccatgcttat ggcgtcggcg aagcggatct tcagcagata
     1621 gaaataattg ctgatgaact ccacctcttc ccggaggagg acgaggtcgc gttccttgtt
     1681 gctgaggata tagcggtata cccgggcgag ggtgtcgtta tagagccggg cgttctttgg
     1741 gtcgcgggtg atcaggaagg acagggtatt gagggaattg aagatgaagt gggggtcgat
     1801 ctggttcttg agggcttcca gttcggcctg cgccttggcg acgttgagtt gttccacgcg
     1861 ggattcgttg tattcttttt cgaggttgag gaagaggatc tcgtagatat tggtgatgaa
     1921 acaggcggcg atgatgatga tgagtacggt attgacgagg tgttcgttgt cgccctgggg
     1981 ttcgcgggag cagaactgcc agaggcggag gaggagcagg gagatggcgc cggagtagac
     2041 gatattggcg aagaagagcg cgacgatgat cttataatag gatttttttg accaggggaa
     2101 tttttcccgg atatagtaca tgaggcggac attgccttcc cagacgagga aggcgacgac
     2161 tatgaagaag aggtagcagg ccgccagttc ggagtagcta tagaggcgat ttgtgacaag
     2221 accggaaagg ttagggatga acaggcccag gacagggatg cctatgcttt ttaccaaccg
     2281 gtcgtttaag acgatctgtt tattcatagc aggttagggc ccaggcggcg gcgtctgagc
     2341 gactattttt tgattgttct gggaagtggt aagcgacggg ctatcccgtt agttcgttgg
     2401 tgtagtagat tggaataacg gcaagtaatc catttgtgct gtgatagaaa agttaattta
     2461 ttgcaaaagg aatttgggga gattacgaac gggcgcgcaa caggatcagc ggtgccgacg
     2521 ttagtatcat tcatacctta aaagaaacca ttagacacgg aaatatcccg tttgagtcag
     2581 tttacccatc tgccgatgtc aaatgtggtt atctttccgt gtgtgactta attaaactca
     2641 ttccggggga aaggtatttc actaatgggc aacttccccg gagtctaaaa caagttgtcc
     2701 ttagaagttt tctcatgtga cccgcaggtg gtttctacca cctgctcttt ttttctcccg
     2761 aacgccctgt tttttgtccc gaaatgccgg gtttccggct tgttcgtgcc ggccttattt
     2821 gttttcgggg ttgttcctac ctgtttccct gtttgttcct atttgtttcc acttgtacga
     2881 gcgtgtacgg gtttgttcat tcttcaatgg ttccgctttc tttttcaatt tctccgcttg
     2941 ttttcttcaa ctgtagcgat aatgggccca ctgtgttgtc gcttcttttt taacctacca
     3001 ttttgatagg gttaccaatt tagtccttgt ataacggggt tgggacagtt gttttccagc
     3061 ttgggtatct ctgttgaaag ttcgtgcgtc ggttgtctta ttttttgtgt cttttttgaa
     3121 cggcccatcg ggtgagatcc ctgtgtgccc tgagttaacc tacattttta ccattacaaa
     3181 accaacagca aatgagaaag cttgttctat ttcttgtagc gagcctgctg gtatcggtgc
     3241 tggtgaaggc acaaacccgg gaggtgaccg ggaaggtgac cgattcagcc ggtattccgt
     3301 tagcgggtgc atctattaat gtcagaggat cgagggtggg caccagcgcc ggtccggatg
     3361 gcagtttccg g
//
LOCUS       DATGFG010000655         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1495631, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000655 DATGFG010000000
VERSION     DATGFG010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495631"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VKQ52_12245"
     CDS             complement(<1..464)
                     /locus_tag="VKQ52_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ88012.1"
                     /translation="MHVELTDKEIIGRILHGEQALFAQLVQRYQQYVFTLVLRFTDNR
                     EDAEEVSQDVFVKAYRSLADFRGEAKFSTWLYTVVRTSCITFLRKKKLATTSMDNERT
                     LIQLENRESGFNADTIEQKSRHAMVNAAIRLLSPDDAQLITLFYKGEQTLEE"
     gene            637..1050
                     /locus_tag="VKQ52_12250"
     CDS             637..1050
                     /locus_tag="VKQ52_12250"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HLZ88013.1"
                     /translation="MGPDELVFVWLIFLSLSIGAVIFGIRYMRNRENMAMIEKGLDPN
                     IKPERPRPAPFRSLKWGLLLVGAGLGLFLAYLLDNTLLYRVGHYVSRYGGGDFEVNGA
                     NVSVYFALIAIGGGMGLITSYRIEKKELLDKQDKS"
     gene            complement(1121..2713)
                     /locus_tag="VKQ52_12255"
     CDS             complement(1121..2713)
                     /locus_tag="VKQ52_12255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLZ88014.1"
                     /translation="MKKLQVWLPLLFAIVMIVGMLIGFRLRGNIRPTGIFMTRKPTPV
                     QEVMDLINMNYVDSVSTDTIGNDAIEGMLSHLDPHSVYIPAVDAVEMNEDLQGNFEGI
                     GVEFQIFDDTVNVMNVLAGGPSDKANLKVGDKFLKVGDSVVAGNGITNDRIRRLLRGP
                     GASQVTVSLLRADNGNTPLQVHITRGTIPLPSVDVAYMINKETGYIRINRFSETTHAE
                     FAQAIMQLQKQGLQRLILDLRGNGGGILQESIECADDFLDGDKLITYTVGARSPKAEY
                     RCRRDGLFEKGKLVLLTDEGTASASEVLTGALQDWDRATIIGRRTFGKGLVQQPFQLS
                     DGSTLRLTIARYYTPTGRSIQKPYDKGHDDYNEEVMKRFHDGEVLHGDTTTRHAGPAY
                     RTLGPEKRIVYGGGGITPDIFIGFDTSTFSHAATNLYVDGTINRFIYVYYIQHLSTFR
                     NFKAPADFIAGFHDEEGLWRSLSAYAPQDKLASIPAKEKDILKQRLKALMARQIWRTE
                     GYYEVGNAYDPVVSKALEVVSK"
     gene            2963..>3321
                     /locus_tag="VKQ52_12260"
     CDS             2963..>3321
                     /locus_tag="VKQ52_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88015.1"
                     /translation="MNILIADSGATKCEWCLLQDGKRKKVDTQGISPYFLDAAGIGEV
                     IRLELLPGLKKAPVDVVYYYGTGCISIDNRRTVQKAIRQAFPGAKVHVTHDLMGAAIA
                     LCGSSKGMVCNLGTGSFS"
BASE COUNT          664 a          759 c         1079 g          819 t
ORIGIN      
        1 atctcttcga gggtctgttc gcccttgtag aagagggtga tcaactgggc gtcgtcgggg
       61 ctgaggagcc ggatcgcggc gttgaccatg gcatgtctcg atttttgttc gatggtgtcg
      121 gcgttgaatc ccgactcccg gttctccagc tgtatgaggg tgcgttcatt gtccatcgat
      181 gtggtagcca attttttttt tcgcaggaag gtgatgcaac tggtacggac cacggtatac
      241 agccaggtgc tgaatttggc ctccccgcgg aagtccgcca gggagcggta ggcttttacg
      301 aatacgtcct ggctgacctc ttcggcgtct tcgcggttat cggtaaaacg aaggacaagg
      361 gtgaatacgt attgctggta ccgttgcacc aactgagcga acagcgcctg ttctccatgc
      421 aggattcttc caatgatctc tttatcagtg agttcaacgt gcattgctag ttatgagacg
      481 acctgatgac gcctcaggtt acaaaggaag gtacaaaaaa taaaggaggg ttttttgtgg
      541 ggagcactgt aacctgtact ttccgggcgt agtctgagag agtgtataac atcgcgccga
      601 agcgcgagga atttgtaaaa gtaaaaaaat aatgaaatgg gaccagatga actagtattt
      661 gtatggttga tattcctatc tctttcgatc ggcgccgtca ttttcggcat ccgctatatg
      721 cgaaaccggg agaatatggc catgatagaa aaggggcttg accccaatat aaagcccgag
      781 cgtccgcggc ctgcgccttt ccgcagcctg aaatggggtt tgttgctggt gggggccggg
      841 ttgggattgt tccttgccta cctgctcgac aacacattgt tgtaccgggt ggggcattat
      901 gtgagtcgct atggcggcgg ggattttgag gtcaatggcg ccaatgtgag tgtttatttt
      961 gcgcttattg ccatcggcgg cgggatgggg ttgatcacct cttaccggat cgagaagaag
     1021 gaactgctcg acaagcagga caaatcgtag tagttattaa tggtttgatt aaagttgcgg
     1081 accggctttt cagccggtcc ttttttattc ccgtctttct ttacttggag accacttcga
     1141 gggccttgct tacgacgggg tcgtaggcgt tgcctacttc gtaatagcct tcggtgcgcc
     1201 agatctgccg ggccatcagt gctttcagcc gttgtttcag gatatctttt tcctttgcgg
     1261 ggatcgatgc cagcttatcc tgcggggcgt aggcggagag ggaccgccag agtccttctt
     1321 cgtcgtggaa gccggcgatg aaatcggccg gggctttaaa attccggaag gtggacaggt
     1381 gttgaatata atagacgtag ataaagcggt tgatggtgcc gtccacgtag aggttggtgg
     1441 cggcgtgcga gaaggtgctt gtatcgaagc cgatgaagat gtcgggggtg atgccgccgc
     1501 cgccgtagac gatccgtttt tcggggccga gggtgcggta ggcggggccg gcgtgtcttg
     1561 tcgtggtatc accgtggagg acttccccgt cgtggaagcg tttcattact tcttcgttgt
     1621 agtcgtcgtg gcctttgtca tagggttttt ggatgctccg gccggtgggg gtatagtaac
     1681 gggcgatggt gaggcggagg gtggagccgt cgctcagttg gaagggttgt tgtaccaggc
     1741 ctttgccaaa ggttctgcgg ccgatgatgg tggctctgtc ccagtcctgg agggcgccgg
     1801 tgagcacctc gcttgccgag gcggttcctt catcggtgag caggaccagt tttccttttt
     1861 cgaagaggcc gtcgcggcgg cagcggtatt ccgcttttgg cgagcgggcg ccgacggtat
     1921 aggtgatgag tttgtcgccg tcgaggaagt cgtcggcgca ttcgatggat tcctggagga
     1981 tgccgccgcc gttgccccgg agatcgagga tcaggcgttg gaggccttgt ttctggagtt
     2041 gcatgatggc ctgggcgaat tcggcgtggg ttgtctcgga gaagcggttg atccggatat
     2101 agccggtctc cttattgatc atataggcga cgtcgacgga ggggaggggg atggtgcccc
     2161 gggtgatatg cacctggagg ggggtattgc cattatctgc ccgaaggagg ctgacggtga
     2221 cctggctggc gcctggtccg cgcagcaatc tgcggatgcg gtcgtttgtg atcccattgc
     2281 ctgcgacgac ggagtcgccc actttcagga atttgtcccc gaccttgagg ttggccttat
     2341 cgcttgggcc gccggcgagg acgttcatga cgttgacggt gtcgtcgaag atctggaatt
     2401 cgaccccgat gccttcaaaa ttgccctgga ggtcttcgtt catttcgacg gcgtcgacgg
     2461 cgggtatata gaccgaatgc gggtcgagat ggctgagcat gccttcgatg gcgtcgttcc
     2521 cgatggtgtc ggtggagacg gagtcgacat agttcatatt gatcaggtcc atcacttcct
     2581 gcacgggggt tggtttgcgg gtcatgaaga tgcctgtggg gcggatattc ccccggaggc
     2641 ggaaaccgat aagcatgccg acgatcatga cgatggcgaa taacaagggt agccagacct
     2701 gcaatttttt cattcctaca atatttttaa caaaggacgc cgccggcggt cccggccgaa
     2761 ggccaattaa ttagccggat tgaccggccg ggtttaaatt tcgaaaaaac ccggccggca
     2821 atcgccagaa ggctgcaaat taatgaatca taagttaaat tgcaggctaa aagctgatag
     2881 acggcgggcc gggccggaac cgggtatttt gcggccgggt cagcgaaccc gccggccgga
     2941 gggaagatca aaagataggc caatgaatat actgatcgcg gatagcgggg ccaccaaatg
     3001 cgagtggtgc ctgcttcaag acggaaagag aaagaaagtg gatacacagg ggatcagccc
     3061 ctatttcctg gatgccgccg gcatcggtga ggtgatccgg ctggaattgt tgccggggtt
     3121 gaagaaggcg cctgtggatg tcgtttatta ttacggcaca gggtgtataa gtatcgacaa
     3181 ccggcggacg gtgcaaaaag ctatccgcca ggcctttccg ggagccaagg tacatgtgac
     3241 ccatgacctt atgggggcgg cgatagcgtt gtgcgggtct tcaaagggga tggtctgtaa
     3301 cctgggcacg ggcagttttt c
//
LOCUS       DATGFG010000656         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1346586, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000656 DATGFG010000000
VERSION     DATGFG010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346586"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VKQ52_12265"
     CDS             <1..418
                     /locus_tag="VKQ52_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HLZ88016.1"
                     /translation="FQTRYAKIGVYICYDRHFPDGARVLGLNGAEIVYNPSATVAGLS
                     QYLWKLEQPAHAAANGYYMGCINRVGEEKPWNLGRFYGSSYFVDPRGQIFAQASEDKD
                     ELLIAEFDLAKIDEVRSVWQFYRDRRPETYGRLTEL"
     gene            433..1785
                     /locus_tag="VKQ52_12270"
     CDS             433..1785
                     /locus_tag="VKQ52_12270"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ88017.1"
                     /translation="MAIHNHRLTDEEYARNFADIHPPFETRDGALVDANRCLYCYDAP
                     CTKSCPTGIDVPKFIKQIVTDNLRGSAHTILQSNIMGGGCSRVCPVEKLCEGACVYNL
                     MEEAPIPIARLQRYPTEKAIREKWPLFERSAPTGKKVAIVGAGPSGLSAAHLLARAGV
                     EVTIYEKEQKGGGLMTYGIAAYKVTPAFCAEELDYILSIGGITILYDRELGRNLSLAH
                     LRKQYDAVYLAFGVGLARRLDIPGEDLEGVIDAIRFIYAIRAEGYPAVPVGDKVAVIG
                     MGMTAIDAATQARRLGAKEVTLVYRRTQAEMPCTEAELDIAKLDGCRLVWLAAPKAIL
                     SDGDQGRVTRLICSVMEPGPPDASGRRSPVDTGQTITLEVDMVIKAAGQIPFETLVSS
                     EAIGNNKGRVSVDKSATTIEGVFAGGDNVNGGKEVVDAVQAGKDAAAAMLRYINNKQQ
                     "
     gene            1810..>2102
                     /locus_tag="VKQ52_12275"
     CDS             1810..>2102
                     /locus_tag="VKQ52_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88018.1"
                     /translation="MASLQTDFLGIRSPNPFWLASAPPTDKQINVLRAFEAGWGGVVW
                     KTLGSQIKNVSSRYSAVDYHHSRIMGFNNIELISDRPLDLNLREIKECIRLFP"
BASE COUNT          469 a          703 c          561 g          369 t
ORIGIN      
        1 cttccagacc cgttatgcaa agatcggcgt gtatatctgc tacgaccggc atttccccga
       61 cggcgcacgg gtcctgggtc ttaacggtgc ggagatcgta tacaatcctt ccgctaccgt
      121 ggcggggctg tcacaatatt tgtggaaact ggaacagcct gcccatgccg cggccaacgg
      181 ctattacatg ggttgtatca accgtgtcgg cgaagagaag ccctggaatt tagggcgctt
      241 ctacggcagc agctatttcg tggatccccg cggccagatc ttcgcgcagg cctccgagga
      301 caaggacgag ctgctgatag ccgaattcga tctggcgaag atcgacgagg tgcgcagcgt
      361 ctggcagttc taccgcgatc gccggcccga aacctatggc agactgacgg aactataacc
      421 aaaatcattg ctatggcgat ccacaatcac cgcctcaccg acgaagaata cgcccggaat
      481 ttcgccgaca tccatccccc cttcgaaacc cgggatggag ccctagtcga cgccaaccgc
      541 tgcctctatt gttacgacgc cccctgtacc aaaagctgtc ccaccggcat cgatgtgcca
      601 aaattcatca aacagatcgt taccgacaac ctcaggggct cggcgcatac gatcttgcaa
      661 tccaatatca tgggcggcgg ctgctcccgc gtctgccccg tggagaaact ttgtgaaggc
      721 gcctgcgtgt acaacctgat ggaggaagcc cccataccca tcgcccgtct ccagcgctat
      781 cccacggaga aggctatccg ggaaaaatgg cccctgttcg aacgaagcgc ccccacggga
      841 aaaaaggtgg ccatcgtcgg agccggtccc tcgggactta gcgccgccca cctcctcgcc
      901 cgcgccggcg tggaggtcac catctatgaa aaggaacaaa aggggggcgg cctgatgact
      961 tatggcatcg ccgcatataa ggtaacaccc gccttctgcg cggaggagct ggactatatc
     1021 ctctccatcg gcggcattac catcctgtat gaccgggagc tgggccgtaa cctcagcctt
     1081 gcccatctgc ggaaacaata tgatgccgtc tacctcgcct ttggtgtggg tctcgcccgt
     1141 cgtctcgata tacccggcga agacctcgaa ggcgtcatcg acgccatccg gttcatctat
     1201 gccatccgcg ccgaaggcta tcccgccgtc cctgtcggcg ataaagtagc cgtcatcggc
     1261 atggggatga ccgccatcga cgcggctacc caggcgcgcc gcctcggcgc gaaagaggtg
     1321 accctcgtct accgccgcac ccaggccgag atgccctgta cggaagccga actggatatc
     1381 gccaaactcg acggctgccg cctcgtctgg ctggcggcgc caaaagcgat cctgtcggac
     1441 ggcgatcagg gccgcgtaac ccggctcatc tgcagcgtca tggaaccggg accacccgat
     1501 gccagcggcc gccgcagccc cgtcgacacc ggccaaacga tcaccctcga agtggacatg
     1561 gtcatcaagg ccgccggaca gatccccttc gaaacactcg tcagcagcga agcgatcggt
     1621 aacaacaagg gccgcgtctc cgtcgataaa agcgccacca ccatcgaggg cgtcttcgcc
     1681 ggcggcgata acgtcaacgg cggtaaagag gtggtcgatg ccgtacaggc cggcaaagat
     1741 gccgccgctg ccatgctccg gtatatcaac aacaaacaac aatgatcacc atcaaaatgc
     1801 cgaacaacta tggctagtct ccaaaccgat ttcctcggca tccgctcgcc aaaccctttc
     1861 tggctggcca gcgcgccacc caccgacaaa cagatcaacg tcctccgcgc cttcgaagcc
     1921 ggctggggcg gcgtcgtctg gaagaccctg ggcagccaga taaagaacgt ctcctcccgc
     1981 tactccgccg tcgactatca ccacagccgc atcatgggat tcaataatat cgaactgatc
     2041 agcgaccgcc ccctcgacct caacctgcgc gagataaaag aatgcatccg cctcttcccc
     2101 ga
//
LOCUS       DATGFG010000657         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1376196, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000657 DATGFG010000000
VERSION     DATGFG010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376196"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..887
                     /locus_tag="VKQ52_12280"
     CDS             126..887
                     /locus_tag="VKQ52_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ88019.1"
                     /translation="MSGNNPQMFTGVADPRTPYMLRENGNGTYKGFIPWLGTSGLAAG
                     DYPQNFWGNATATSTTSPAVNNSRYIFNDTAEYPMMTASEMQLILAESYLRNNDQANA
                     LTAYTKAIQLNMDMLTTIYPRNIPAAKVMTPASEAAYLANPKVVPAVGGLTLSHIMLQ
                     KYIALYGWGVNETWTDMRRFHYTDADPVTGNQVYANFVVPSGINIYATNGGKPVYRCK
                     PRYNSEYLYDVPELTRIGAENPDYNTYECWFSQKN"
     gene            906..1709
                     /locus_tag="VKQ52_12285"
     CDS             906..1709
                     /locus_tag="VKQ52_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88020.1"
                     /translation="MKKLKSIILLSSLAVITGVFSCKRDMALRDPAVPVSVNSANISL
                     IDVAPNLSTVLGVKADTFNVLFNNVKVTGYTAGTSPVMTFSGMYPLSGTAAGYAAIPA
                     GDQTIKFARGVNTLDSLVLFTFNESLQANSYYTLLITDSIKSGNDAARMFLKDNFSAV
                     DTGFYNMRFVNAVLNDTAGVDVWSARQAKNIYTNVKPGNVVDFAKFNSSWTADTLFCR
                     RAGTAVGLDTLLTQNFYSLRTYTLVYKGNAFSAVKTDTKRRHLISYVNK"
     gene            complement(1805..>2137)
                     /locus_tag="VKQ52_12290"
     CDS             complement(1805..>2137)
                     /locus_tag="VKQ52_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88021.1"
                     /translation="LGGIRHAASSLGMDTRWVGGMPTALIKPLTAAGARGMLVDAMAH
                     NVDSFAARLSGIMQGSSETAFYVVAVYFGSVSIRNTRYALPAMLLAELVSVISSIFLC
                     YLFFPGIQ"
BASE COUNT          534 a          665 c          512 g          426 t
ORIGIN      
        1 tccgccaaca ccgacaatgc gatgtgcaag ttccagggcg gcaccacctc gcccgtaaac
       61 agctactacg ggccctaccg ctccaacctg ggaagctatc gccagggtaa tatatcgccg
      121 acctgatgtc gggaaacaac ccccagatgt tcaccggcgt cgccgatccc cgcaccccct
      181 atatgctgcg ggagaacggc aacggaacat ataaaggatt cattccctgg ctcggtacca
      241 gcggcctcgc cgccggcgac tacccgcaga acttctgggg caacgctacc gctacatcca
      301 ccacatcgcc cgcggtcaac aacagccggt atatcttcaa cgatacggcc gaatacccca
      361 tgatgaccgc atcggaaatg cagctcatcc tggccgaatc ctatctccgg aataacgacc
      421 aggccaatgc attgacggcc tacacaaagg cgatccagtt gaacatggat atgctgacga
      481 ccatctatcc acgcaatatt ccggccgcca aagtaatgac gcccgcaagc gaagccgcct
      541 acctcgccaa ccccaaagtt gtgcccgccg taggcggtct gaccctttcc catatcatgc
      601 tgcaaaaata tatcgcgttg tatggctggg gcgtaaatga aacctggaca gatatgcgaa
      661 gatttcacta caccgatgcg gatcccgtca ccggcaacca ggtctatgcc aattttgtcg
      721 tcccttccgg catcaatatc tatgcgacca atggcggaaa gccggtttat cgctgcaaac
      781 cccggtataa ctcggaatac ctctacgatg tgcccgagct cacacgcatc ggggctgaga
      841 accccgacta caacacttac gaatgctggt tctcacaaaa aaactaaccg tcaaaatctg
      901 gaaaaatgaa aaagctcaaa agcatcatcc tactttcttc cctggcggtc atcaccggcg
      961 tcttctcctg caaaagggat atggccctga gagatcctgc ggtgcccgta tcggtgaatt
     1021 cggccaatat ctcgctgatc gatgtggcgc ccaacctcag caccgttctt ggggttaagg
     1081 cggacacctt caacgtcctt ttcaataacg taaaggttac cggttacacc gccggcacct
     1141 ccccggtaat gaccttcagc ggcatgtacc ccttatccgg tacggctgcc ggttacgccg
     1201 ccatacccgc cggggatcag accatcaagt tcgccagagg cgtcaacacg ctggattcgc
     1261 tggtgttgtt caccttcaac gagagcctgc aggcgaattc ctattacacg ctactgatca
     1321 ccgactccat caaatcgggc aatgacgcag cacggatgtt cctgaaagac aatttctcgg
     1381 cggtcgacac cgggttttac aatatgcgtt ttgtcaatgc ggttctcaat gataccgctg
     1441 gcgtcgatgt ctggtccgcg cgacaggcga agaacatcta cacaaatgtt aaaccaggca
     1501 acgtcgtcga ctttgcaaaa ttcaattcca gctggaccgc cgacacgctg ttctgccgca
     1561 gggcgggcac ggcggttggc ctggacacgc tgttgaccca gaacttctac agcctcagga
     1621 cgtataccct cgtctataaa ggcaatgcat tcagcgctgt aaagacggat accaaacggc
     1681 ggcatcttat ttcatatgtg aacaaatagt atggttatgg ttcttattgc gcaggacacc
     1741 ctgtgagaac gtcacatgag aatggttcgt taataccggc ccgccccatc aggggcgggc
     1801 tcttttactg gatgccggga aaaaataaat aacaaagaaa gatggagctg atcacgctga
     1861 ccagctcggc cagcagcatg gcgggcaggg cataccgcgt attgcggatc gatacactcc
     1921 caaaatagac cgccactaca taaaaagccg tctcagacga cccctgcatg atccccgaca
     1981 gccgggcggc aaaactatcg acgttatgcg ccatcgcatc caccagcatc cccctcgctc
     2041 ccgcagccgt caggggtttg atcagcgccg tcggcatgcc gcccacccac cgtgtatcca
     2101 tgccaagcga ggaagccgca tgccggatcc cgcccaa
//
LOCUS       DATGFG010000658         5206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1373551, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000658 DATGFG010000000
VERSION     DATGFG010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373551"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3707
                     /locus_tag="VKQ52_12295"
     CDS             <1..3707
                     /locus_tag="VKQ52_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLZ88022.1"
                     /translation="FPGYPFPLEEQGAGDDPVDLEEQRTGGDLVYVAMGTILNKDVEL
                     YRTCIGVLSQVGYRAILSVGSKVDMVQLGPMPASIRAAEYVDQRAVLTEAALFITRGG
                     MASVHEAIHTLTPMIVIPVIPEQQMTAERIQELGIGIVLPPGQVTGESLQSAIRQILT
                     NRQGYIERIKALQNDEPVLSVPAKARGLLQDFFRSAPPAGKTLVDLFLSQAERIPDAI
                     ALRCGDKQLTYGQLHGKTNKLAHYLSDLGIGSGVAAVVVLEPCLEMLVAILGILKAGG
                     CYIPADPEYPPERIDYVLKDSACAVVLSKRTVLEKCRLQTAPALVLLDEPIPQPATAS
                     GSCPVSGPSAADPAYIMYTSGSTGRPKGVIVEHGQIYRYITDVHRRLQLEECASYAIL
                     GTFSADAGLTAVFSALCYGKLLHIVDIKAFTSITSLTRYFRQSPVDCYKITPSLLARL
                     LANEGGHAILPVKRLLLGGEACPRTLANTLYTLLPPGCRLFNHYGPTETTVGVTTYAF
                     PDRVQDLPAMIPLGKPLPAVLAHVLDQDGGYVPAGDPGELYIEGSLIARGYLNNPDLT
                     HQKFTTIVLSGQKRRLYRTGDWVRRLPDGNLEYLGRMDHQVKILGNRIELQEVEEVIL
                     ASGIVQTCVVLAKENRQGSPYLAAYIQPRQGFEKDKLLRSVRAKLPSFMVPGSWIPME
                     EWPLTLNNKIDRLALPDPEKQEPSNGDTLFPAGGEIEPVLLDIWMQLLGVPQIGPRDD
                     FFQLGGNSLQLLQLSFEIHNRLSIGISAAELFDYSTLERLSARLSETGRPILPPANPA
                     AEGFDRTEATLAQRNFFIQNKLYPLSAFPNSSLTCEILGELDRPKLEKALLSLINRNE
                     SLRTSYHLSKGKVHSSEDAVSTFQVEYLQCLSNEIDREILEAVRPFTLSQAPLIRVLL
                     FELRNTRKYLHLYLPHINSDGESLKLIMRDLETQYNDALPAERQQFGYFLEHTHAYFH
                     SGHYEEDEAFWARQLHADIPPLHFNFDHPGKNPGPAKLDGVFAVVPFPENFTAALQRY
                     QKEKNSTKFQVLFISFFLLLYKISSVDDLTVLLPVHGRDKKGFEGIIGLLSNVIPVRL
                     RFDPGQGVTDCIQSCKRAILLAISHQQYPFELIHRIWAKKGRRSTSLRQSFFGYHDHR
                     REYTLGSTPLKIYIPERDKEDLPLSAAIFETSQGLTMRISSTAGGFNGGELRRLTDLY
                     FTIAAQLIESEAGDTVGSLLEQAELSLQNS"
     gene            3728..4477
                     /locus_tag="VKQ52_12300"
     CDS             3728..4477
                     /locus_tag="VKQ52_12300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ88023.1"
                     /translation="MEAVSWFTKEEDWLLKRPIIFNKNLVKLTFIEVGRLLKLLGNPA
                     KNSKILDLCCGIGRHSLEFARHGLQVTGVDITRPYLEIAAENAKKEGLSLEFVQSDMR
                     DFCQPDTFDIVVNLCTSFGYFDDMEDDLRVLGNIYRMLTANGKFMIEILGKEVIAATF
                     RSLEELEFEGYKVRAVSRVLENWNRLECRRIITKGDMEKEIIAYHRLYSATEMQGHLE
                     RVGFKNIRVFGDFSGAPYDNEAKSMILVADK"
     gene            4489..>5206
                     /locus_tag="VKQ52_12305"
     CDS             4489..>5206
                     /locus_tag="VKQ52_12305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLZ88024.1"
                     /translation="MEKLHLQPEEIALNLNGRMELPSREEFYTNYPFYKYWNASNNRK
                     LLSPEGINLYIHIPFCIQICDYCFYMKELIKSKDQVDDYIDSLCLEIKMMSEKFSLGS
                     RRVNSIYIGGGTPSVLTEKQFRKLIETLNRYHLIEDPEFAFEAEPGTFNKSKLEWLKE
                     CGVNRISMGIQSFDDEVIRLSSRKHTAGQAINSIAIVKEVGGFELNIDLLSGLAGESM
                     QTWERSVQTALEQETDMLTIY"
BASE COUNT         1328 a         1396 c         1383 g         1099 t
ORIGIN      
        1 cctttcccgg ctatcctttc ccgttggagg aacagggggc cggcgacgac ccggtcgatt
       61 tggaggaaca gagaaccggc ggcgacctcg tctatgtagc catgggcacc atactgaaca
      121 aggatgtgga attatacagg acatgtattg gggttttaag ccaggttggt tatcgcgcta
      181 tcctgtcggt gggctcaaag gtggatatgg tacaactggg accgatgcct gcgtctatcc
      241 gggcggccga atacgtcgac caacgagccg tccttacaga agcggcgttg tttataaccc
      301 gcgggggcat ggccagtgtg cacgaggcga tccacacctt aacgccgatg atcgttatac
      361 cggtgatccc cgagcagcag atgacggccg aaaggataca ggaattgggc attgggatcg
      421 tcctgccgcc cgggcaggta accggggaaa gtctgcaatc agccatccgg cagatcttaa
      481 ccaacaggca agggtatatc gagcggataa aagccctgca aaacgatgag ccggtcttat
      541 ccgtcccggc gaaagcccgc gggttgctgc aggatttttt ccgttctgct ccgccggccg
      601 ggaaaaccct tgtagatctt tttctttcgc aggccgaaag gatcccggac gccattgccc
      661 tgcgctgcgg ggacaagcag cttacctacg gacaactgca cgggaaaacg aataagcttg
      721 ctcattacct gagcgatctg ggaatcggca gcggcgttgc ggcagtcgtg gtgctggaac
      781 cctgcctgga gatgctggtt gccattcttg gcatcctgaa ggcgggagga tgttatatac
      841 cggccgaccc ggaatatccc ccggaaagga tcgactatgt tctgaaggac agtgcgtgtg
      901 ctgtagtgct cagcaaaaga acggtcctgg agaaatgccg gctgcaaacg gccccggccc
      961 tcgttctgct ggatgaacct attccgcagc cggcgacagc ctccggctcc tgcccggtgt
     1021 cggggccatc cgccgccgat cccgcatata tcatgtatac ctcgggctcc accggaaggc
     1081 caaagggtgt catcgtagag catggacaga tctaccggta tattacagac gtccaccgcc
     1141 ggctgcaact ggaagagtgc gccagttatg ccatcctggg aacattttcc gcggatgcgg
     1201 gtcttaccgc cgtcttttcc gccttatgct atgggaagct cttacatatc gtcgacataa
     1261 aagcctttac cagcattacg agcctgaccc ggtatttcag gcaatcccct gtggactgtt
     1321 ataagatcac cccttccctg cttgcccggc ttttggcaaa cgaaggggga catgccatac
     1381 ttccggtgaa gcgattatta ctgggaggag aggcctgtcc ccggaccctg gcgaacaccc
     1441 tctataccct attgccgccg ggctgccggc tattcaatca ctatggacca accgagacta
     1501 cggtaggcgt caccacctat gcctttccgg accgggtgca ggaccttccg gcgatgatcc
     1561 cgttgggcaa accgctcccc gccgtcctgg cgcatgtcct ggaccaggac ggcggatacg
     1621 taccggcagg cgacccgggc gagttataca tcgagggatc gctgatcgcc agggggtacc
     1681 tcaacaaccc ggacctgacc catcaaaaat ttacgaccat cgtcctttcg ggccaaaaaa
     1741 ggagattata tagaacagga gattgggtaa ggaggctgcc ggacggaaac ctggaatacc
     1801 tgggaaggat ggatcaccag gtgaagatcc tcggcaaccg gatcgaacta caggaggtgg
     1861 aggaggtgat actggcaagc ggcattgtac agacatgtgt tgtgctggca aaagaaaaca
     1921 ggcagggttc tccttacctg gccgcgtata tccaaccccg gcagggattc gaaaaagaca
     1981 agctcctgcg atctgtcagg gcgaaactgc cgtcgtttat ggtcccggga agctggatac
     2041 ccatggagga atggccgttg accctcaata acaagatcga ccggctggca ttgcccgatc
     2101 ccgaaaagca ggaaccctcc aacggcgaca ccctcttccc ggcgggcggg gaaatagagc
     2161 ccgtactgct ggacatctgg atgcaattgt taggagtgcc ccagataggg ccaagggacg
     2221 atttctttca actcgggggg aactcattgc aactgcttca gctaagcttc gagatccata
     2281 accggcttag catcgggatc agcgccgcgg aactatttga ctatagcacc ctggagcgac
     2341 tttcggcaag gctgtcggaa accggccgcc ccatccttcc gccggcaaat cccgccgcag
     2401 aagggtttga taggacggaa gccacccttg cccagcgaaa tttctttatc caaaacaaac
     2461 tgtatcctct cagcgccttc ccaaactctt ccctcacctg cgagatcctg ggggaattgg
     2521 ataggccaaa gctggaaaag gcgcttcttt cccttataaa caggaatgaa agccttcgaa
     2581 ccagttatca cctttccaaa ggaaaggtac attcgagcga agacgcggtg tcgaccttcc
     2641 aggtagaata tttacaatgc ctttcgaacg agatcgacag ggagatcctg gaagccgtgc
     2701 ggcctttcac cctaagccag gctcctttga taagggtact cttatttgaa ctccgtaaca
     2761 cccggaaata cctccatctc tatctccctc atatcaactc ggatggcgaa tccctgaaat
     2821 tgatcatgcg ggacctggaa acccaataca acgacgcgct gcccgctgaa aggcaacaat
     2881 tcggttattt cctggaacac acacatgctt atttccattc cggtcactat gaggaagacg
     2941 aagctttttg ggcccggcaa cttcatgccg acatcccccc actgcatttc aatttcgatc
     3001 atccgggtaa aaaccccggg ccggctaaat tggacggggt tttcgccgtc gtaccctttc
     3061 ccgaaaactt caccgccgcc cttcagcgat accaaaaaga gaaaaacagc acaaaattcc
     3121 aggtgctttt tatctctttc ttcctcctcc tgtataagat cagctccgtc gatgacctga
     3181 ccgtgctgct gccggtgcat ggcagggata aaaaagggtt cgaaggcatc atcgggcttc
     3241 tttcgaatgt catcccggta cggcttcgtt tcgaccccgg ccagggagta acggactgta
     3301 tccaatcctg caaaagagcg atcctcctgg cgataagcca ccaacaatac ccctttgagc
     3361 tcattcatcg gatctgggcg aaaaaagggc gcagatcaac gagtctgcgg caatcctttt
     3421 tcggctacca cgatcaccgg agagaatata ccctgggaag caccccgctt aaaatatata
     3481 tcccggaaag agacaaggaa gacctgccgc tatccgcggc catattcgaa accagccagg
     3541 gattgaccat gcggatatcc tcaaccgccg gcggattcaa cggcggggag cttcgaaggt
     3601 taacagacct gtatttcact attgccgccc aactgatcga aagtgaagcc ggtgacaccg
     3661 tgggctccct gctggagcag gcggaactat ccctgcaaaa ttcttaacac actaaatact
     3721 gcaaatcatg gaagctgtct cttggtttac aaaagaggaa gactggttgc tgaaacgccc
     3781 gatcatattc aacaagaatc tcgtcaagct gacttttatc gaggtaggcc gactgctgaa
     3841 actgctcggc aacccggcca aaaacagtaa aatacttgac ctgtgctgtg ggatcgggcg
     3901 acactccctg gaatttgcca ggcacggact gcaggtgacc ggtgtggata tcaccaggcc
     3961 ttacctggaa attgccgccg aaaatgcgaa aaaagaaggt ctttcccttg aattcgtgca
     4021 aagcgatatg cgggactttt gccagcccga cacctttgac attgtcgtca atctctgcac
     4081 ctccttcggc tatttcgatg atatggaaga tgatctgagg gtgctcggca acatctaccg
     4141 gatgctgacc gcaaacggga aattcatgat cgaaatactg ggaaaggaag tgatcgcggc
     4201 caccttccgc agcctggagg aactggagtt cgaagggtat aaagtgagag ccgtcagcag
     4261 ggtcctcgaa aactggaaca ggcttgaatg caggcggatc attacgaaag gagacatgga
     4321 aaaagagatc attgcttacc accggcttta ctcggcgacg gaaatgcaag gccacctgga
     4381 aagggtggga tttaaaaata tccgcgtatt cggggatttc tccggcgcac cctatgacaa
     4441 cgaagcaaaa tcaatgatac tggttgcaga caaataaata tacttattat ggaaaagctt
     4501 cacctccagc cggaggaaat cgcactaaac ctgaatgggc ggatggaatt accttcaaga
     4561 gaggagttct ataccaatta tccgttttat aaatactgga atgccagtaa caatcgcaaa
     4621 ctactgtcgc ccgaagggat caatctttat atccatatcc ctttctgcat ccagatctgc
     4681 gattattgtt tctacatgaa ggaactgata aaatcgaagg accaggtaga cgactatata
     4741 gactccttgt gcctggagat caaaatgatg agtgagaagt tcagcctcgg gagcaggagg
     4801 gtcaactcca tttatatcgg tggcggtacc ccatccgtac tgacggagaa gcagtttaga
     4861 aaactgatcg aaaccctgaa ccggtatcac ctgatcgaag atcccgaatt cgcattcgaa
     4921 gccgagccgg gaaccttcaa taaaagcaag ctggagtggc ttaaggaatg cggggtaaac
     4981 aggatcagca tgggcatcca gtccttcgat gatgaagtga tccgcctgag cagcagaaag
     5041 cataccgccg gccaggcgat caattccatc gccatcgtca aggaggtggg cgggttcgaa
     5101 ttgaatatcg acctgttaag cgggcttgcg ggagagagca tgcaaacatg ggaacggagt
     5161 gtacaaacgg ccctggagca ggagaccgac atgttgacga tctata
//
LOCUS       DATGFG010000659         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_390292, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000659 DATGFG010000000
VERSION     DATGFG010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390292"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VKQ52_12310"
     CDS             <1..523
                     /locus_tag="VKQ52_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ88025.1"
                     /translation="QISINQYIEKETGALRYPSLVANAGYNYSRTQNSVGFSLLNLNY
                     GPYAGITLNVPIFNGNIYKKQQEAAGINVKNARLVRDTLLLNYTSNAVKNFQAYTNNL
                     RQVETAKANYDLSLKLLDLVMKRFQLRQATIVDVKNAQQSFENAGFLLVNVNYAAKAA
                     EIALRRYANQLTY"
     gene            complement(573..1091)
                     /locus_tag="VKQ52_12315"
     CDS             complement(573..1091)
                     /locus_tag="VKQ52_12315"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLZ88026.1"
                     /translation="MATTLEIASTYYQSPLGLLRIGGTDTYISEISFVDHLEETDYNR
                     PRLQGPVPPIVIQCIEQLIQYFQGQRRVFDFPVYQQGTPFQQRVWNELTAIPFGKTLS
                     YLELSRRLGDTKAIRAAASANGRNNIAIVIPCHRVIGSKRDLVGYAGGLWRKKWLLDH
                     ETKIMYGVQTLF"
     gene            complement(1127..1462)
                     /locus_tag="VKQ52_12320"
     CDS             complement(1127..1462)
                     /locus_tag="VKQ52_12320"
                     /inference="COORDINATES: protein motif:HMM:NF007495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HLZ88027.1"
                     /translation="MATIEWQDFEKIDLRAGTIIEVNDFPKAKKPAWQLTIDFGDLGI
                     RKSSAQITDLYEKQELVGKQVIAVVNFPPKQVANFFSECLVLGVYTDKKEVVLLTTDK
                     KVTNGWKIG"
     gene            1502..1924
                     /locus_tag="VKQ52_12325"
     CDS             1502..1924
                     /locus_tag="VKQ52_12325"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLZ88028.1"
                     /translation="MKRTATAAWSGSGKDGNGHLTTQSTTLNNAQYSYKTRFEQGVGT
                     NPEELIAAAHAGCFTMALAFQLQGAGFTPDSLETTATINFDVAAQPATISQSALAIKG
                     KVPGITNEKFQELVKAAEQGCPVSRVLKCEITSQATLL"
     gene            2115..3554
                     /locus_tag="VKQ52_12330"
     CDS             2115..3554
                     /locus_tag="VKQ52_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ88029.1"
                     /translation="MSTKKSSTPGKLQASRREFLRGAAMATTGFFIVPRHVLGRGFLA
                     PSDRLTVAAIGAGGKGHDDLRNFYGSGKVDVAYLCDVDDRQAAASVKEYPKAKYYKDF
                     REMLDKEHKHFDAVSVSTPDNCHAAAAIAAMQLNKHVYVQKPLTHDIYEARMLTEAAK
                     RYKVVAQMGNQGSSGDGVRLMQEWYNAGLIGDATAIHVWTNRPVWPQGVAKPTGKEEI
                     PKELDWDLWQGPAQADDYHKLFLPFNWRGWWAYGTGALGDMGCHLIDPPFKTVGLGYP
                     NAVECSISSTYEVMWNPAYHPESCPTSSYVKMNFPGKPGKPDVALHWMDGGIRPVRPD
                     ELGADEPMGNDDGGAIITGTKGKIMCNCYGAKPTLLPTSRMSEVSSVPQTLARVPEGH
                     YVQWVNACIAGYGQKQLSSPFEYAGPLTETILMGNLALRSYMIKDANGHFPGRKKLLW
                     DAENMKITNFDAANQFVKREYRQGWKLGL"
     gene            3787..4329
                     /locus_tag="VKQ52_12335"
     CDS             3787..4329
                     /locus_tag="VKQ52_12335"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HLZ88030.1"
                     /translation="MKNFHAGWYLIYTKPRHEKKVHTRLTEMKINSFLPLTKKLRIWH
                     DRKKIINEPLFPSYVFIYLDDLQAYYEGIDTDGSLYYVRTGKEIARVNDIVINNIRIL
                     VDKGSEIEVLTENIQPSQEFLIRQGPLTGLSCEIVQVSGSHKVLVRVHLLQRNLLVTL
                     PSDYLTQPLHSEEPASRRHT"
     gene            4349..>4675
                     /locus_tag="VKQ52_12340"
     CDS             4349..>4675
                     /locus_tag="VKQ52_12340"
                     /inference="COORDINATES: protein motif:HMM:NF016377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ88031.1"
                     /translation="MKIPRILNQTWKDHDVPADFKEMADSWRKMHADWQYVLWTDEMN
                     RNFVRQHFPYFLPTYDGYETNIQRVDAVRYFVLYKMGGFFIDLDFECLANIEPILANA
                     ECVFGKE"
BASE COUNT         1118 a         1129 c         1346 g         1082 t
ORIGIN      
        1 gcagatctcg atcaaccagt atatcgagaa agagaccggc gccctccgtt atccttcgct
       61 ggtcgcaaac gcaggttaca actacagccg tacacagaac agcgtaggtt tttcgctgct
      121 gaacctcaac tatggacctt atgcggggat cacgctgaat gtgccgatct tcaatgggaa
      181 tatttataag aagcagcagg aggcggcggg tatcaatgtg aagaatgccc ggcttgtgcg
      241 ggataccctg ttgttgaact atacctcgaa tgccgtcaag aatttccagg cgtatacgaa
      301 taacctgcgg caggtggaga cggcaaaggc gaattacgat ctttcgctga agctgctcga
      361 cctggtgatg aagcgtttcc agttgcggca ggcgacgatc gtggatgtca agaatgcgca
      421 gcagagtttt gagaatgcgg gtttcctgct ggtgaatgtg aattatgcgg cgaaggcggc
      481 ggagattgcg ctcaggaggt atgcgaatca gttgacgtat taggtcgttg ccccgaggct
      541 cgcctggaac cggacgggta cgcaatgggc cgttagaaga gcgtctggac gccgtacatg
      601 atctttgttt cgtggtcgag cagccatttt ttgcgccaga ggcccccggc gtagccgacg
      661 aggtcgcgtt tggagccgat gacgcggtga caggggatga cgatggcgat gttgttgcgg
      721 ccgttggcgg aggcggcggc gcggatggct tttgtatcgc cgaggcggcg ggagagttcg
      781 aggtaggaga gggtctttcc gaaggggatg gctgtcagtt cgttccagac ccgttgctgg
      841 aagggagtac cttgctgata gacggggaag tcgaagacac ggcgttgtcc ctgaaagtac
      901 tggatgagtt gttcgatgca ctggatcacg atcgggggga cggggccctg gagcctgggg
      961 cggttgtaat cggtctcttc gaggtgatcg acaaagctga tctcgctgat ataggtatcc
     1021 gtgccaccga tgcggagcag gccgagcggg gactggtaat acgtcgaagc gatctccagc
     1081 gtggttgcca ttgttgctga tttggggtgg gtcccggggt gaggagctag ccgatcttcc
     1141 agccgttggt caccttttta tccgttgtca ataaaactac ttcttttttg tcagtataca
     1201 cacccaggac gaggcattcg ctgaaaaaat tggccacctg ttttgggggg aagttgacca
     1261 cggcgatgac ctgcttaccc acaagttcct gcttttcata caggtctgtt atttgtgcgg
     1321 aggattttcg gatgcctaaa tcaccgaaat cgatggtcaa ttgccaggcc ggcttttttg
     1381 ctttgggaaa atcattaact tcaattattg ttccggcacg gaggtctatc ttctcgaaat
     1441 cctgccattc tattgtcgcc atggttccag ttctttttat cttaattaaa tatacaattt
     1501 tatgaaacgt acagcaacag ccgcgtggag tggctccgga aaagacggta acggccattt
     1561 aacgacacaa agcacgaccc tgaacaatgc ccagtactcg tacaagacgc gtttcgagca
     1621 aggagttggg accaatcccg aggagctgat cgccgcagca catgccggct gcttcacgat
     1681 ggcgctggcg ttccagctgc agggggccgg atttaccccg gacagcctgg agacgaccgc
     1741 cacgatcaat tttgatgttg ccgctcagcc ggcgaccatt tcccaatcgg cgctggcgat
     1801 caagggaaag gttccgggta tcaccaatga aaaattccag gagctggtca aggctgccga
     1861 gcagggctgc ccggtgagcc gtgttctgaa atgtgagatc acttcgcagg caaccttgct
     1921 gtaaggcatt aacgccaaat taattttgca atttttagca ccttcgggtg cttcccgcgc
     1981 gggcgcagcc ccgggacaat atgctaaaaa agcgctccaa atttttgttg ctggagcgct
     2041 ttttttgtgt gtgaggttca gtaacttacc cacgcaaatt ttgcgactat ttaaactcag
     2101 caacacgtta cattatgtcc acaaagaaaa gttctacccc cggcaagctg caagcatccc
     2161 gccgggaatt cctgcgcggc gccgcgatgg cgaccacggg tttttttatc gtgccacggc
     2221 atgttcttgg cagggggttc cttgctccca gtgaccggct gacggttgcc gccattggcg
     2281 ccggcggaaa gggccatgac gacctccgga atttttacgg cagcgggaag gtggatgtcg
     2341 cctatctctg cgatgtcgat gaccgtcagg cggcggcttc cgtcaaggag tatcccaagg
     2401 cgaaatatta caaggatttc cgcgagatgc tggacaagga gcacaagcat ttcgatgcgg
     2461 tgtccgtatc gacgccggat aattgtcatg ccgcagccgc cattgccgcc atgcagttga
     2521 acaagcatgt gtatgtacag aaaccgctga cgcacgatat ctatgaggcg aggatgctga
     2581 ccgaagccgc gaagcggtac aaggtagtgg cccagatggg caaccagggt tcatcgggag
     2641 atggggtgcg gctgatgcag gagtggtaca atgccgggct tatcggcgat gccacggcca
     2701 tccatgtctg gaccaaccgg cccgtgtggc cgcagggggt tgccaaaccg acggggaagg
     2761 aggagatccc gaaggaactg gactgggatc tctggcaggg acctgcccag gcggatgact
     2821 atcataaact attcctgccg ttcaactggc ggggctggtg ggcctatggt acgggtgcgc
     2881 tcggagatat gggctgtcac ctgatcgatc cgcccttcaa aaccgtgggg ctgggttatc
     2941 cgaatgccgt ggagtgcagt atcagctcca cctatgaggt catgtggaac ccggcctatc
     3001 atcccgaaag ctgccccacg tcttcctatg tgaagatgaa cttccccggc aaaccaggta
     3061 aaccggatgt agccctgcac tggatggatg gtggtatccg gcccgtgcga ccggatgaac
     3121 tgggggccga tgagccgatg ggcaatgacg acgggggtgc gatcatcacc ggaacaaagg
     3181 gaaagatcat gtgcaactgc tatggcgcca agcctacgtt gctgcccaca tcccggatgt
     3241 ccgaggtgag cagtgtgccg cagaccctgg cacgggtgcc ggaaggccac tatgtccaat
     3301 gggtgaatgc atgtatcgcg ggatatggcc agaaacagtt gagctcacct ttcgagtatg
     3361 cgggaccgtt gacagaaacg atactgatgg gcaacctggc gttacggagc tatatgatca
     3421 aggacgcgaa cgggcatttc cccggcagga agaaactgct ctgggatgca gagaatatga
     3481 agatcacgaa tttcgatgca gccaaccagt ttgtcaagcg ggagtaccgc caaggctgga
     3541 agttaggtct ctaataccga agcgatccaa atttaataaa gagggtatcc ctgatcaggg
     3601 ataccctctt ttagtaaaaa ccgaaataag aaatgatggt actactaagc gacaagttaa
     3661 tatacttcat ttaatttgtc ccaacctgtt gatttgttat cctttgatct gcccggggag
     3721 atgttacttg ctgcagcgat agctgtaatc cggaaccagc aatctttcca ataaaaatat
     3781 attacgatga aaaatttcca tgccggatgg tatctgatct atacgaagcc gcgccatgaa
     3841 aaaaaggttc atacccgact gacggaaatg aaaatcaatt ctttcctgcc tttaacaaaa
     3901 aagctgcgga tatggcacga tcggaaaaaa attataaatg aaccgctttt cccttcctat
     3961 gttttcatct acctcgatga cctgcaagcc tattatgaag gtatcgatac ggacggcagc
     4021 ctctattatg tcaggacagg aaaggagatc gcgagggtca acgatatcgt gatcaacaat
     4081 atccggatac ttgttgataa aggaagcgag atcgaggtgc tgaccgagaa tatccagcca
     4141 agccaggaat tcctgatacg gcagggtcct ttgacggggc tttcctgcga aattgtccag
     4201 gtgagcggca gccataaggt actggtcagg gtacatctcc tgcaaaggaa tttacttgtc
     4261 accttaccct ccgattacct gacgcagccc ctgcattctg aagagccggc atcgcggcgg
     4321 catacctagc gaaacatccg agttttatat gaagatccca cgtatcttga atcagacatg
     4381 gaaggaccat gatgtccccg cagacttcaa agaaatggcg gattcctggc gtaaaatgca
     4441 tgccgattgg caatatgtgc tctggacgga tgagatgaac aggaatttcg tcaggcagca
     4501 tttcccttat tttttgccaa cctatgacgg ctatgagacg aatatccagc gtgtcgatgc
     4561 cgtgcgatat tttgtgttgt acaaaatggg cggcttcttt atcgacctgg atttcgaatg
     4621 cctggcgaac atcgagccca tcctggccaa tgccgaatgt gtttttggga aggag
//
LOCUS       DATGFG010000660         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_940520, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000660 DATGFG010000000
VERSION     DATGFG010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940520"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2506
                     /locus_tag="VKQ52_12345"
     CDS             <1..>2506
                     /locus_tag="VKQ52_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88032.1"
                     /translation="DTALVRAFDWAKAQALHYRGKPADPVGPWYESALPPRDAFCMRD
                     VSHQAIGGAILGLDAENKNMLTLFAKNITAPKNWCSWWEMNKHGVPAPEDYRSDKEFW
                     YNLDANFDLLWATWRLGAWTGDSSYYEDPVFRRFQQKTTGAYIDSWVLQPDSLLTRPA
                     HPNAPIPFNEQDAFDRCRGLPSYSEGIPNMKMGVDLVAALYRGLRTYADILRQRGQPS
                     AANTFARRAAQYRSHLETRWWNDSLGRYQTWYSTDNHFGLGEGETFLLWFDALQDTAR
                     LRRTIDHLASVRWNMENTSYLPYLFYREGSWDTARQTLLYLADPATERREYPEVSFGV
                     IQAVVLGLMGVDPIPGTRTVTTIYRHRGPGSAWLERLPILGTTLTVRHASPLETTVAN
                     TGRRRLIWRAQFSGSFTSARVSTSSASARVGGKKMPMQRLADKWGRIISYVDLPLDAG
                     QQMTVTVSHGRTGLASTPSNPESPPHTYKNSSPALRKAIEACIRTKERVLNLPGGRID
                     LWPEGAVQKELYISNATEDDTLSKQKHIAICLENVHHLLIQGHHTLLVLHGKMLSFAL
                     LNCSDITIRDIKVDYERPTMSEMTILSVQPHEAEVLIHKDSRYRIDEAGKIHFYGEGW
                     ETRNFHTIVYDPADETMRYSSFQPFGESNATATAPLQVHFQGDFSKTGLHAGEVLTFR
                     DPYRDNVGAFIGQSRNVLLHNVDMYYMHGLGIVSQYSENLRFIGVHITPRPGSGRVIS
                     AFADCFHFSGCKGSILLDSCYTKGSHDDAVNIHGTHLRIVSIAGSAAGNGGATSPGST
                     NAADAATPSAPNILRVQFMHPQTWGFKAFYPGDSIAY"
BASE COUNT          548 a          817 c          681 g          460 t
ORIGIN      
        1 gacaccgcgc tggtgcgggc tttcgactgg gcaaaggcgc aagccctgca ttacaggggg
       61 aaaccggcgg atccggtggg cccctggtat gagtccgcac tgccgccccg ggatgcgttc
      121 tgcatgcggg acgtatccca ccaggcaatc ggcggcgcta tcctgggtct cgacgcggaa
      181 aacaagaata tgctgacgct gttcgcgaag aacatcaccg cccccaaaaa ctggtgctcc
      241 tggtgggaga tgaacaagca cggcgtaccc gcgccggagg actaccgcag cgacaaggaa
      301 ttctggtata acctggatgc taatttcgac ctgctatggg ccacatggcg gctgggcgcc
      361 tggacaggcg acagcagcta ctacgaagac cccgtattcc ggcggtttca gcagaagacc
      421 accggcgcct atatcgacag ttgggtgctg caacccgact ccttgttgac acgacccgcc
      481 cacccgaatg cacccatacc ctttaacgag caggacgcct tcgaccgttg tcgcggcctg
      541 ccatcgtatt cggagggcat ccccaatatg aaaatgggcg tcgacctggt ggccgcgctt
      601 taccgcggtc tccggacata tgcggatatc ctgagacaac gcggacagcc gtccgcggcc
      661 aacacctttg cgcgacgtgc ggcgcaatat cgctcccacc tggagacccg ctggtggaat
      721 gattctctcg gccgctatca aacctggtac agcaccgata accattttgg actcggtgag
      781 ggcgagacct tcctcttgtg gttcgatgcc ctgcaggaca cggcccgcct tcgcaggaca
      841 atcgaccacc tggcgtcggt gcgctggaat atggaaaata cttcctacct gccctatttg
      901 ttttaccgcg aagggtcctg ggatacggcg agacagaccc tgctctacct ggccgatccg
      961 gccaccgaac ggcgggaata tccggaggta tcctttggcg tcatacaggc ggtggtcctc
     1021 ggtctgatgg gggtggaccc cataccgggc acccgcacgg taacgacaat atatcgacac
     1081 cgcggccccg gaagcgcctg gctggaacgg ctacccatcc tgggcacgac cctcaccgta
     1141 cgacatgcct cgcctctgga aaccaccgtc gccaacacgg gccgccggcg actcatctgg
     1201 cgggcgcagt tctccggctc attcacaagc gccagggtct ccacctcctc cgcgagcgca
     1261 agggtagggg ggaaaaagat gccgatgcaa cgacttgcgg acaaatgggg ccggatcatc
     1321 tcctatgtgg acctgccgct ggacgccgga caacaaatga ccgtcaccgt tagccatggt
     1381 cgcaccggcc tggcatccac tccatccaac ccggaatccc cgccgcacac ctataaaaac
     1441 tccagccccg ccctccgcaa agccatcgaa gcctgcatcc ggacgaagga aagggtacta
     1501 aacctacccg gcggccgcat cgacctctgg cccgaagggg ccgtccaaaa ggaactctac
     1561 atctccaacg ccaccgagga cgataccctg tcaaaacaaa aacacatcgc gatctgcctg
     1621 gaaaatgtac atcacctcct tatacaagga catcataccc tgctggtact gcacggcaaa
     1681 atgctgtcct tcgcattact gaactgcagc gacatcacga tccgcgacat aaaggtcgat
     1741 tatgagcgcc cgaccatgtc cgaaatgacg atcctgtctg tccagccgca cgaggccgaa
     1801 gtcctcatcc acaaagattc acgctacagg atcgacgaag ccggcaagat ccacttttac
     1861 ggagaaggct gggagacgcg gaatttccat accatcgtat atgatcccgc cgacgaaacg
     1921 atgcggtact cctcgttcca gcctttcggg gaaagcaacg ctactgccac ggcaccgttg
     1981 caggtgcatt tccagggcga tttcagcaag accggactcc atgcaggcga ggtgcttaca
     2041 ttccgcgatc cctaccggga taacgtcggc gcctttatcg ggcagagccg gaatgtgctg
     2101 ctgcacaacg tcgacatgta ctatatgcac ggactgggca tcgtttccca atactccgaa
     2161 aacctccgtt tcatcggcgt tcacatcacc ccccgccccg gctccggccg cgtcatcagc
     2221 gccttcgcgg actgctttca tttttccggc tgcaagggca gcatcctgct ggacagttgt
     2281 tacacaaagg gctctcacga cgatgcggtg aatatccacg gcacacatct gcgcatcgtt
     2341 tccatcgcgg gcagcgcggc cgggaatggt ggggcaactt cgccgggttc gaccaatgcg
     2401 gccgatgcgg ccactccgtc ggcccctaac atcctccgcg tccaattcat gcatccgcag
     2461 acctggggtt tcaaggcatt ctatcccggc gacagcattg cgtata
//
LOCUS       DATGFG010000661         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1656558, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000661 DATGFG010000000
VERSION     DATGFG010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656558"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1215)
                     /locus_tag="VKQ52_12350"
     CDS             complement(79..1215)
                     /locus_tag="VKQ52_12350"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding peptidase"
                     /protein_id="HLZ88033.1"
                     /translation="MKVYTCSNCQNPLYFENSVCLNCRHAVGFDAVELTMITLKPESA
                     GKSGIFSHVSKENELYKYCKNAEYGVCNWLLPASSASGFCIACTLNRVIPTLDGDVNK
                     GRWKNLEVAKHRLIYSLLRLRLPFEVGGSVNGVASDAQRMANGARGATGDEKEESIAF
                     DFMADTPAGERVVTGHDNGVITINIAEADEVQRVRNKQDLGERYRTLLGHFRHEIGHF
                     YWDVLIRDRAVIDQFRLLFGDERQDYAAALATYYSNGAPADWNQHFVSPYAAAHPWED
                     WAETWAHYLHLMDTLETAWSFGLSIDPVEVDEGAGISASIVKDPYGVKDFNRLIKRWL
                     PLCFAVNSLNRSMGHPDFYPFILSSPVIEKLRFIHGICQSGVKS"
     gene            complement(1271..2149)
                     /locus_tag="VKQ52_12355"
     CDS             complement(1271..2149)
                     /locus_tag="VKQ52_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HLZ88034.1"
                     /translation="MKYHISHQTTYRYYAPVSLCYNLLRLAPRNTPSQLCTRSEVKIF
                     PEPDSLQEYKDFFGNKVIYFSIEKEHAQLSVKVYSEIEKRVAAAREIGLYGNATIESV
                     KQQLAENRQEAEEIGQYIFETPMTAGNAEIAAYARASFSPGRPLLEAAGDLMRRIYTD
                     FRFTPGFSTVSTPLETVLRERKGVCQDFAHLAIACIRSVGLPARYVSGYIETIPPPGT
                     EKLTGVDASHAWFSVYIPQLGWVDFDPTNNVQPAEQHITIGWGRDYADIAPMRGVLLS
                     SGTHGLSVSVDVSRVR"
BASE COUNT          475 a          718 c          623 g          493 t
ORIGIN      
        1 cgccgtttcc ggcgcagcgg ttgggccgcg caatagccgg tctggtctcc gcatagaatg
       61 cccggttccc cctggctcct acgacttcac gccgctctgg cagatcccat gaataaaccg
      121 aagtttttcg atcacggggg aagagaggat aaacggataa aaatcggggt gccccatact
      181 gcggttgagg ctattaacgg cgaagcatag gggcagccat cgtttgatga gccggttgaa
      241 gtccttcact ccataggggt ctttgacgat gctggcgctg atgcccgccc cctcatccac
      301 ttccacggga tcgatcgaga ggccgaagga ccaggccgtt tccagcgtat ccatcaggtg
      361 tagataatgt gcccaggtct ccgcccaatc ctcccaggga tgggccgcgg catagggact
      421 cacaaaatgc tggttccagt cggcgggcgc tccgttcgaa tagtaggtgg ccagcgcggc
      481 cgcatagtcc tggcgttcgt cgccgaaaag cagacggaac tgatcgataa cagcgcggtc
      541 cctgatcagc acatcccaat agaaatgccc gatctcatgg cgaaaatgcc ccaggagcgt
      601 ccggtagcgc tcgccgaggt cctgtttatt ccgcacccgc tgcacctcat ccgcctccgc
      661 gatattaatg gtgatgaccc cgttgtcatg gcctgtcacc accctttccc cggcgggcgt
      721 gtccgccatg aagtcaaagg cgatggattc ctccttctcg tcgccggttg ctcccctcgc
      781 accattggcc attcgctgcg cgtcgctggc caccccattc acgctgcccc ccacctcgaa
      841 gggaagccgc agtcgcagca atgaatagat cagccggtgt ttggccactt ccaggttctt
      901 ccaccgcccc ttgttgacgt ccccatccag cgtggggatc acgcggttca gcgtgcaggc
      961 gatgcaaaag ccggatgccg aagaagcggg cagcagccag ttgcagactc catactccgc
     1021 gttcttgcag tatttatata gttcgttctc cttcgataca tgggaaaaaa tgccgctctt
     1081 tccggcggat tccggcttca gcgtgatcat cgtcagctcc acggcatcga agcccacggc
     1141 gtgccggcaa ttcaggcata cactattctc gaaatacagg gggttctggc aattgctaca
     1201 ggtgtatact ttcatggctg gcttatgaat cctgggaaat gcaacaacta taccacggga
     1261 accggcattc tcatcgcacc ctactcacat ccaccgacac cgacaaccca tgcgtcccgc
     1321 tactaagcaa gactccccgc atcggcgcaa tatccgcgta gtcccgtccc cagccaatgg
     1381 tgatatgctg ttccgccggc tgcacattgt tggtggggtc gaaatccacc cagcccagct
     1441 gcggaatata gacgctgaac caggcatgag acgcatccac accggtgagc ttctcggtgc
     1501 ccgggggcgg gatcgtctcg atgtatccgc tgacataccg cgccggcaga cctaccgatc
     1561 ggatacaggc gatcgccagg tgcgcaaaat cctggcacac ccccttgcgc tcgcgcagga
     1621 ctgtctccag cggagtactc accgtcgaaa agccgggggt aaaccggaaa tcagtataga
     1681 tccggcgcat gagatcaccc gccgcctcca gcaagggcct gccgggtgaa aaggatgccc
     1741 gcgcataggc cgcgatctcc gcgttccctg ccgtcatcgg ggtctcgaaa atatattgcc
     1801 cgatctcttc cgcctcctgc ctgttctccg ccagctgctg ctttacgctt tcgatagtgg
     1861 cattcccgta caggccgatc tctctcgctg ccgccacgcg cttctcgatc tccgaataga
     1921 ccttcaccga cagctgcgca tgctcctttt cgatggagaa atagatcacc ttattaccga
     1981 aaaaatcctt gtactcctgc aggctgtcgg gctcgggaaa aattttcacc tcactgcggg
     2041 tacacagctg cgaaggagta ttcctcggcg ccagccgcag caggttgtaa caaaggctca
     2101 cgggtgcgta gtatctgtag gtcgtctgat ggctgatgtg atacttcata gattcattga
     2161 aggtcgaagc ttcgctgggt gtcctcgtgt ttaaaatagg ttttggagat cacctccggg
     2221 atggcggcca gcaggccata gatccgggat aagaattcgt ccagccgggt gtagatcgcg
     2281 gcttcgcttt cctccacagg cagaaggtc
//
LOCUS       DATGFG010000662         4419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_606597, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000662 DATGFG010000000
VERSION     DATGFG010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606597"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(573..1316)
                     /locus_tag="VKQ52_12360"
     CDS             complement(573..1316)
                     /locus_tag="VKQ52_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ88035.1"
                     /translation="MPIKTVIVEDEEKSLCVLKELIRISAADIEVLGVASHVEKAVDL
                     IESIAPQLVLLDVRIADGLGFEVLKRLHKRDFQLICITAYDNYAVAAFRYSAIDYLLK
                     PVGMEDWQEMLRRVRTRLAERDDVDRVGALLHRLDDPVRKKLTLSTLNGFDFVDLADI
                     VWCNSEGGYTVFHLADRSKRVSSRNLGAYEAQLCEEGFFRIHHSCMINLRCIKSYKKG
                     KGGYVVMCNNVELEVSQRRKADFLRKLAP"
     gene            complement(1307..4285)
                     /locus_tag="VKQ52_12365"
     CDS             complement(1307..4285)
                     /locus_tag="VKQ52_12365"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ88036.1"
                     /translation="MRVVVLLFSWLITAALADGQELSYHRFTVKDGLPGSIVYHCLQD
                     AKGFIWFATDQGVSRFDGRNFRNYTREDGLPDNDILKLYLDRFDNVWFLSFIGIPSVL
                     YHDSIISFNNCRGVRSICEDTRNDCIQLMMADELSGTNGCYRGPDHPGRWKFSPDITS
                     MNVAYPHPLLRVSTPGNTDFFLSGRGVAAGETLKVKEGGVTASFLFNRNDKEFSPFAR
                     QWGCSVLSNGRSIVFLAVDSAYYSDIGRLSPLFSLRSLVSRQNLTIPEVNDVFGEDDS
                     TLWICTRNYGLLRVRNFLDSRHTVDEFFPGHFCTSMIRDREGGYWVTTYSDGVFYCPN
                     LCFRAVSSPHDLNFASVRSLRLMDTTHIVAGFDNGNIMVFDRHSLRHRVFPRWAISNR
                     NNRIMDLAPLGRHSLVVAADRGLYRLSFADTEARLRPGAFKELFVRSDDSLLAANAEG
                     AMLLDTTGRLLRPILSPRATCLAGFGDLLYWGGHAGIHVFQQGRSRLFGEQYPGLTGV
                     INHIDIAPDSAIWISTEEGLMIWKAGAMHHIGKEQQLISNLCKQVSFHGTTAWVATDK
                     GISRLDYHWQDTGLHYSAFSITEEDGLRADNVNRIVIAGGTVWAATTRGISSFPEDYT
                     GRSTLRPGINITRIAVDDEPLALKDSAAVTYRSGKLLVELAGISFRSGGHVAYEYRLT
                     GIDNNWNSLTGNTIDIHALPFGRFVLQARAIDRWGARSLPISIMILHPSPFYRSAWFI
                     VSFYLFFTLLTAAGLYMFNRHRQRKKDKEFRLRKKMHELEIAALRAQINPHFIFNCLT
                     SIQYHILRADVLSANAYLHKFSNLIRLTLQCSTLPIVSLEEEIRMLTLYLELEKLRLG
                     DRMNYRLVLPPNFNPEGLSFPSMIVQPYVENAIQHGIAPLEGRIGELAIEFTLTAGYL
                     CCTVEDNGPGIGRNRLRQTDRTHIPLGTSLTSRRLGIINSINKQKIHLEIIDKSLAGS
                     AGSGTIVHLRFPLKTDYSCL"
BASE COUNT          921 a         1200 c         1351 g          947 t
ORIGIN      
        1 tggtctgggc gttccccaaa accattcccc agactgtaac acttcctacc cggcatattt
       61 ccttcccata catacgacgc ctgcggcgtc tgccagggct aaagtcgggc cttcggccgg
      121 ccttcctccg gtcggccctg ccgcttgcgg gcacctacca tggcctctgg gatcaagcga
      181 tccggcgttg tctagcgccg gatatttttt ttgcccgtgg tctgggcgtt ccccggcgcc
      241 gccccggcta tcgccggggg gcgagtccct cgccctcgct tcgcgaggcc ccggcgccgg
      301 gtcgggctct ccgctgtagt cctcgccctt cggtcttcgg gctgccgctc cgttccctaa
      361 cgcggctccc tcagctccca ccactcgccg ccccgcaaat ttctctctta ttcatacgac
      421 gcctgcggcg tctgccgggg agaacttcgt cgaagctccg agctctcgct tcgactcgtt
      481 cgcaaccttc gctgcgctcg gttgggtcgg gccttcggcc ggcctccctc cggtcggcct
      541 tggctcccta acgcggctcc ctgccgcgcc cgtcatggcg ccagctttcg caggaaatcc
      601 gctttgcggc gttgcgagac ctccagctct acgttgttgc acatgacgac ataccctcct
      661 tttccttttt tatagctttt gatgcatcgc aggttgatca tgcaactgtg gtggatgcgg
      721 aagaagcctt cttcgcacag ttgcgcctcg taggctccca ggttgcggga ggaaacgcgc
      781 ttggatctgt cggcgagatg aaagacggtg tatccgccct cggaattgca ccagacgata
      841 tcggcgagat cgacgaagtc aaagccattg agcgtggaga gggtgagttt cttcctgacg
      901 gggtcgtcga ggcggtggag caaggcgccg acgcggtcga catcgtcccg ctccgccagt
      961 ctcgtgcgca cccgccggag catttcctgc caatcttcca ttcccaccgg ctttagcagg
     1021 tagtcaatgg cggaataccg gaaggcggcc accgcatagt tatcgtatgc tgtaatacag
     1081 attagctgaa aatcgcgctt gtgcagtcgt ttgaggactt cgaaccccag gccgtcggcg
     1141 atgcggacgt ccagcaaaac gagttggggt gcgatggatt caatgagatc caccgctttt
     1201 tcgacatggc ttgcgacgcc aagtacctcg atatccgcgg cggagatccg gatcagctcc
     1261 ttcaggacgc aaaggctttt ttcttcatct tcgacgatga ctgtttttat aggcatgagt
     1321 aatcggtttt aagggggaat cggaggtgaa cgatcgtgcc ggaaccggcg gagccggcaa
     1381 gcgacttgtc gatgatctcc aggtggatct tttgcttatt gatcgaattg atgatcccga
     1441 ggcgcctcga ggtgaggctg gtgcccaggg gtatatgggt tctgtccgtc tgccggagcc
     1501 ggttgcggcc gatgccgggc ccgttgtctt cgacggtaca gcacaggtag ccggcggtca
     1561 gcgtgaattc tattgccaat tccccaatgc gtccttccag cggggcgatg ccgtgctgga
     1621 tagcattctc cacatagggt tggacgatca tcgagggaaa ggaaaggcct tccgggttga
     1681 aattgggtgg cagcaccagc cggtaattca tgcggtcgcc cagccggagt ttctcgagtt
     1741 ccaggtaaag ggtaagcatc cggatctcct cctccaggga aacgatgggc agggtactgc
     1801 attgcaatgt caggcggatg agattggaaa atttatgaag ataggcatta gcgctgagca
     1861 cgtcggcccg gagaatatgg tattgaatgg aagtgaggca gttaaaaatg aaatgcgggt
     1921 ttatctgagc gcggagcgca gcaatctcga gttcgtgcat cttcttcctg agacggaatt
     1981 ctttgtcttt cttgcgttgc cggtgacggt tgaacatata cagcccggcg gcggtgagca
     2041 gggtaaagaa gaggtagaac gaaacgatga accatgccga ccggtaaaac ggggaggggt
     2101 gcaggatcat gatacttatc ggaaggcttc tggcgcccca gcgatcgatg gctcttgcct
     2161 gcaggacgaa acggccgaag ggaagcgcgt ggatatcgat ggtgttaccg gtgagtgaat
     2221 tccaattgtt atcgatgccc gtgagccggt attcgtaggc cacatggcca ccgctgcgga
     2281 aggagatgcc ggccagttcc accagcagtt ttcccgatcg gtaggtgact gccgcgctat
     2341 ctttcaaggc aagtggttca tcatccacgg ctatacgggt aatattgatc cccggccgga
     2401 gtgtggagcg ccccgtatag tcttcgggaa aggaggagat gccccttgtc gttgcggccc
     2461 agaccgtgcc gccggcgatc actatgcggt ttacattgtc ggcccgcaac ccgtcttctt
     2521 ccgtgatgga gaaagcggaa tagtgtaatc ccgtgtcctg ccagtggtaa tcgagccggg
     2581 agatgccctt gtctgttgcg acccaggccg tcgttccatg aaaactcacc tgtttgcaaa
     2641 ggttgctgat cagctgttgc tccttgccga tgtgatgcat ggcgccggct ttccatatca
     2701 tcaacccttc ctccgtcgag atccagatcg cggagtccgg agcgatatcg atgtgattga
     2761 tgacgccggt gaggcccggg tactgctcgc cgaaaaggcg gctccggccc tgttgaaaaa
     2821 catgaatgcc ggcatggccg ccccaataaa gcaggtcgcc gaagccggcc aggcaggtag
     2881 cgcggggaga aaggatcgga cgcaggagcc ggccggttgt atcgagcagc atagcccctt
     2941 ccgcattggc cgccagaagg ctgtcatcac tcctcacgaa gagttcttta aaggcgccgg
     3001 gtcgcaggcg ggcttcggtg tcggcgaatg acagccggta aaggccccga tcggcggcaa
     3061 ccaccaggga atggcgccct aagggggcaa gatccatgat ccggttgttc cggttgctta
     3121 tcgcccagcg gggaaatacc cggtggcgaa gcgaatggcg atcgaaaacc atgatgttgc
     3181 cgttgtcgaa tcctgcaaca atgtgagtgg tatccatgag ccgaagactg cggacactgg
     3241 caaagttgag gtcatgcgga ctggaaaccg cgcggaaaca aagattgggg caatagaaga
     3301 ccccatcgct gtaggtggtt acccaatagc cgccttcccg gtcgcggatc atggaggtac
     3361 aaaaatgtcc gggaaagaat tcgtcgacgg tgtgccggct gtcgaggaag ttgcggacgc
     3421 ggagcaggcc gtagttgcgg gtgcagatcc aaagcgtcga atcgtcttcg ccgaagacat
     3481 cgttcacctc ggggatcgta agattttggc gggaaaccag cgatcgcagg gagaagaggg
     3541 gagacagccg gccgatatcc gaatagtagg cggaatcaac ggcaaggaat acgatggacc
     3601 tcccattgga gagcacgctg caaccccact ggcgtgcaaa gggggagaac tccttgtcgt
     3661 tgcggttgaa taggaaactt gccgttacgc cgccttcctt tactttcagg gtttcgccgg
     3721 ccgccactcc cctgcccgaa agaaagaagt ccgtgtttcc cggggtagac acccgcagaa
     3781 ggggatgggg ataggctaca ttcatcgaag tgatgtcggg ggaaaatttc catcggccgg
     3841 gatgatcggg tccccggtag cagccgttcg tacccgaaag ttcgtcggcc atcatcagtt
     3901 gaatacagtc attacgcgtg tcttcacaaa tggaccgaac gcccctgcaa ttattgaagc
     3961 tgattatcga gtcgtgatac aggacggagg gaatgccgat gaaggagagg aaccatacat
     4021 tgtcgaaccg gtcgagataa agcttgagga tatcgttgtc gggaaggccg tcctctctgg
     4081 tgtagttgcg aaagtttctg ccatcgaagc ggctgacgcc ctgatccgtc gcaaaccaga
     4141 tgaatccctt tgcatcctgg agacaatggt aaacgatgga accgggcaga ccgtccttta
     4201 cggtaaaccg gtgataggaa agttcctgcc catcggccag ggcagccgtg atcaaccagg
     4261 aaaaaaggag aacgactact cgcatagatc ggcggggtta tcggcaaaat taaaccttag
     4321 gcgccgaaag gaggacggct gcggcggcgt ccgtcgctgc ctaggtgttt acacggagga
     4381 tacatcgaaa aaaatcaggc aggtgttgca gttgttggc
//
LOCUS       DATGFG010000663         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1503942, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000663 DATGFG010000000
VERSION     DATGFG010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503942"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..920
                     /locus_tag="VKQ52_12370"
     CDS             156..920
                     /locus_tag="VKQ52_12370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HLZ88037.1"
                     /translation="MAPFAHPSSIVRTIWGRGDTVLLIFAGASAEFALNRSVDWLYFT
                     GKLPADPLGRLFSTVAYAQQIVFAEEDRALAAIDNITAIHRGVEEKRGASIPATAYLD
                     VLFMLIGYSISSFELLERRLTVEEKAEVFAVFERVGRRMGLTGMPEDFGAWERMRARY
                     LKENLAVSNFSHDLYRRYRKSLGAVRYFLLVQAQVLLVPGRVRRLLDLPSVHLLYPVV
                     QVYKLFRWLRLDKGIKILLLPPRYRAQVFGLDQKMG"
     gene            955..>2021
                     /locus_tag="VKQ52_12375"
     CDS             955..>2021
                     /locus_tag="VKQ52_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ88038.1"
                     /translation="MQTRRDFLQKMTGSAIGLSLLTRTASAAQPAIKEEHYDGPMLRV
                     AIMGLGSYGTRVAEAMKSCTKAKLVGVISGTPAKIKAWQTKYNIPEKNCYNYEDFDRI
                     KDNPDIDAVYVITPNGLHHNQVIRVAMAKKHVICEKPMAVNAKEGQEMVDACQNAGVR
                     LLVGYRMHFEPHTLEVIRMRKAGEFGRILFFQGLSGFIIGDPTQWRLNRALSGGGAMM
                     DIGIYSINGSRYMIGEDPVWVTAQETKTNPVKFKEGVDETIQFQLGFPGGAVASCLST
                     YSLNNLDRFFLDGTQGFAELQPATGYGPIQGRTNKGEINQPVTTHQTVQMDEMAGIIL
                     EGHQPVVPVDGQEAVKDLKIV"
BASE COUNT          411 a          507 c          653 g          450 t
ORIGIN      
        1 gccgaccggc tgggttttac aaaaccttcg gtgatccgga cgctgatggg ttatactttc
       61 acctgggtgg atatcctttg ttatacactg ggtatcggga ccgtgctggg gttcgaggcc
      121 cttgcgcggt tgcgcccaaa actaaaatga cggctatggc gccttttgca catccttctt
      181 ccattgtccg caccatctgg ggccgcgggg atacggtcct gctcattttt gcgggggctt
      241 cggcggagtt tgcgttgaac agatcggtcg actggttata ctttacgggt aagcttccgg
      301 ccgatccgct cggcagactt ttttcgacag tggcgtatgc gcagcagatc gtctttgccg
      361 aagaggaccg cgcgttggcg gcaattgaca atatcaccgc gatccatcgc ggggtggagg
      421 agaagcgcgg cgcttccatt ccggcgacgg cctacctgga tgttctcttt atgctgatcg
      481 ggtattcgat cagcagtttc gagttgctgg agagacggtt aacggtggag gagaaggcgg
      541 aggtgttcgc tgtttttgag cgggtggggc ggcggatggg cctgaccggt atgccggagg
      601 atttcggggc gtgggagcgg atgcgggcgc gttacctgaa ggagaacctc gcggtaagca
      661 atttttcgca tgatctttac cgccgctacc ggaagagcct gggcgcggtg cgttatttcc
      721 tgctggtaca ggcccaggtg ctcctggtgc cgggaagagt gcggcgactg ctggatctgc
      781 cttcggtgca tcttctttat cccgtggtgc aggtgtataa gctgttccgc tggctccggc
      841 tggataaggg tataaagatc ctgttgttgc ccccgcgtta ccgggcacag gtgtttgggc
      901 tggatcaaaa aatgggataa cttaggggac tatttcccat attatcccta tatcatgcaa
      961 acacgccgcg atttcctcca gaaaatgacc ggttcggcca ttggcctttc cctgctgacg
     1021 cgcacggctt ccgccgctca gcctgcaata aaagaagagc attatgacgg tccgatgcta
     1081 cgcgtggcca ttatgggcct cgggagttat gggacgcggg tggccgaggc catgaaaagc
     1141 tgtaccaagg ccaaactggt gggtgtcatc agtggcacgc cggccaagat aaaggcctgg
     1201 cagacgaagt ataacatccc cgagaagaac tgttacaatt acgaggactt cgaccggata
     1261 aaggataacc ccgatatcga tgcggtgtat gtcattacac ccaatggcct gcatcacaac
     1321 caggtgatcc gggtggcgat ggcgaaaaag catgtcatct gtgagaagcc gatggccgtc
     1381 aatgcgaagg agggccagga gatggtggat gcctgtcaga atgccggggt ccggctgctg
     1441 gtgggttacc ggatgcattt cgagccgcat acgctggagg tgatccggat gcggaaggcg
     1501 ggggagttcg gcaggatcct tttcttccag gggctttccg ggtttatcat cggagatccg
     1561 acgcagtggc ggttgaacag ggcgctctcg ggtggaggcg caatgatgga catcggcatc
     1621 tattcgatca atggttcccg gtatatgatc ggcgaggacc cggtctgggt cacggcgcag
     1681 gagacaaaga ccaatcccgt aaaattcaag gagggggtgg atgagaccat ccagttccag
     1741 ctgggcttcc ccggcggagc cgtcgcctcc tgcctgtcca cgtatagcct gaacaacctg
     1801 gaccgtttct ttctcgacgg aacgcagggt tttgcggagc tgcagcccgc cacggggtat
     1861 ggaccgatac agggacgtac gaacaagggt gagatcaatc agccggtgac cacgcatcag
     1921 accgtccaga tggatgagat ggcgggtatt atactggaag gccaccaacc ggtcgtaccg
     1981 gttgatggcc aggaggccgt caaggacctc aagatcgtcg a
//
LOCUS       DATGFG010000664         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1390644, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000664 DATGFG010000000
VERSION     DATGFG010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390644"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1569)
                     /locus_tag="VKQ52_12380"
     CDS             complement(100..1569)
                     /locus_tag="VKQ52_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLZ88039.1"
                     /translation="MFPWKSIIQIQKDSATPIYLQIANSMALEIKRGRIGPGIKLPGS
                     RQLSETLTIHRKTIIRAYEELEGQGWIEMRPSRGTFTSKELPEINPRKLPEEKRPGPG
                     NYPNETGYPIRVNTVVKTPGNPMRHIIGFHEGPDARLMPAYELAKAYRSALLKKVNLK
                     YTSYVETAGVEKFRRILSDYLNSSRGLRTSFENILVTRGSTMGLYLIVAALFEKGDTV
                     IVGDTNYYYADRTFLQAGMKLARIPVDDHGIDVDAIEKLCRRKKISAVFITPHHHFPT
                     TVTLSASRRMKLLTLAESAGFVIIEDDYDYDFHYQSSPILPMISADSKGMVIYIGTLS
                     KTLSPAIRTGYVVAPVNLIIELARLRQLIDTQGDPFMELALADMFQDGTIKRLLKKAL
                     QEYHRRRDFLCAALTQNPFAGKIDFRIPDGGLSIWAKFHPAIPLPPLAGKLKEKGIIL
                     SNGLIHNTTSHNLNATRMGFGWMTEKEAEKAIAILGSII"
     gene            complement(1641..>2034)
                     /locus_tag="VKQ52_12385"
     CDS             complement(1641..>2034)
                     /locus_tag="VKQ52_12385"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HLZ88040.1"
                     /translation="FSRIIAAIAGHLSRNHRKLFLNIRTDGFLLDLPTALPETLTRIK
                     AYGNAGADGIFVPCISAPQDIEQVVTATTLPVNVMAIPGLPAFETLQQLGVKRISMGP
                     FLFNKVYAHAATLAAQVIHEKDLAPILS"
BASE COUNT          368 a          446 c          690 g          530 t
ORIGIN      
        1 gatccctttc atattctttt gatttgaatg ttgtacttca aaagtagaaa tgatccggac
       61 tatttagcgg accggttttt agatatcagg tagtccggtt tagatgatgg aaccgaggat
      121 ggcgatggct ttttccgctt ctttttcggt catccagcca aatcccatgc gggtggcatt
      181 gaggttgtgg ctggtggtgt tatggatcag cccgttggaa aggatgatgc ctttttcctt
      241 tagcttccct gcgaggggcg gtagggggat ggcggggtgg aattttgccc agatggagag
      301 gccgccgtcg gggatgcgga aatcgatctt gcccgcgaag gggttttggg tgagggcggc
      361 gcataggaaa tcccggcggc ggtggtattc ctgcagggct tttttgagga gtcgttttat
      421 cgttccgtcc tggaacatat cggcgagggc cagttccatg aatggatcgc cctgggtgtc
      481 gatgagttgt cttaaccggg ccagctcgat gatcaggttg accggggcga cgacatagcc
      541 cgtgcggatg gcgggggaca aggttttgct cagtgtcccg atatagatca ccatgccctt
      601 gctgtcggcg ctgatcatcg ggaggatggg ggaactctgg tagtggaaat cataatcgta
      661 atcgtcctcg atgatgacga agccggctga ctcggcgagg gtgaggagtt tcatccgccg
      721 ggaggcggat agggtgacgg tggtagggaa gtgatgatgc ggggtgatga atacggcgct
      781 tatctttttc cggcggcaga gtttttcgat ggcgtccaca tcgatgccat ggtcgtcgac
      841 gggtatgcgt gccagtttca tgccggcctg gagaaaggtc ctgtcggcgt agtaataatt
      901 ggtatcgccg acaatgacag tatcgccctt ttcgaagagg gcggccacga tgagatagag
      961 tcccatggtg ctgccgcggg tgacgaggat gttctcgaag cttgtccgca gaccccggct
     1021 gctgttgagg tagtccgaga ggatgcgcct gaatttttcc acgccggccg tttcgacata
     1081 ggaggtatat ttcaggttga ctttttttag cagcgcgctg cggtaggcct tcgccagttc
     1141 gtatgccggc atcaggcggg cgtccggtcc ttcgtggaag ccgatgatat gacgcatcgg
     1201 gttgccgggt gtctttacga cggtattgac gcggatggga taaccggtct cgttgggata
     1261 attgccgggg ccggggcgtt tttcttccgg tagttttcgg gggtttatct ccggcagttc
     1321 cttgctggtg aaggttcccc gggagggacg catctcgatc cagccttgtc cttccagttc
     1381 ttcgtaggcc cggatgatcg tttttcgatg gatcgtaagg gtttcggaga gttggcggga
     1441 gccgggcagt tttatgccgg gaccgatgcg gcctcttttt atttccaggg ccatcgaatt
     1501 ggcgatctgc aggtagatgg gcgtggcgct gtccttttgt atctgtatta tcgatttcca
     1561 gggaaacatg acgggttatt cgatcatcga agatagtttt tttgtgggta cgtccgggga
     1621 aaggcggcgg caagggtggg ttatgaaagg atgggggcaa ggtctttttc atggatcacc
     1681 tgtgcggcca gggtggcggc gtgagcatac accttattaa aaaggaaggg ccccatgctg
     1741 atccttttta ccccgagttg ctgcagcgtt tcgaaggcgg gcagcccggg gatggccatg
     1801 acatttaccg gcagggtagt ggcggttacc acctgttcaa tatcctgggg agcgcttata
     1861 cagggaacaa agatgccatc ggcgccggcg ttgccgtagg cttttatccg ggtaagcgtt
     1921 tcgggcagtg ctgtcgggag gtcgaggagg aacccgtctg tccggatgtt gaggaagagt
     1981 ttccggtggt tccggctgag gtgaccggcg atggccgcga tgatcctgct gaag
//
LOCUS       DATGFG010000665         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_583984, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000665 DATGFG010000000
VERSION     DATGFG010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583984"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            697..1008
                     /locus_tag="VKQ52_12390"
     CDS             697..1008
                     /locus_tag="VKQ52_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88041.1"
                     /translation="MLGQRSLFDSIIDVPQAPKNNRGGRDPQLLQRRNEVIVARYYYL
                     VRIKKMQYPEALATLRVQFFHSEIIIQRVLQRHYEQLKRFKEERPPVGVFKKNYPWLV
                     W"
     gene            complement(1013..1516)
                     /locus_tag="VKQ52_12395"
     CDS             complement(1013..1516)
                     /locus_tag="VKQ52_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88042.1"
                     /translation="MKEIYLETLERIESQVPAIKYIDQDFGQIDNVKEGTRAPVGFPC
                     ALIDFDEAQFSNLGNKVQLAEPATLKIRLAFPNWQPGNNLVKEQVRQYAVSMYDTEIA
                     VNQALHGWRGSIFGPLTRTRRETEKRHDYKVIAVTYEFTYKDMTCSPAYQPAPGNTGL
                     GIDIAFT"
     gene            complement(1513..1995)
                     /locus_tag="VKQ52_12400"
     CDS             complement(1513..1995)
                     /locus_tag="VKQ52_12400"
                     /inference="COORDINATES: protein motif:HMM:NF016927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HLZ88043.1"
                     /translation="MGIIQAMKVLAQELKQLQRDAPRYIGDLAVNHFNENFQREGFVD
                     DTLQPWPPREKDKDEGRAILFKTGQLRKSIRVVRKSSQEVVIGSDIKYAKVHNEGGII
                     QVKAHMRKSKTGKELPIRAYMYKAQQRKFMGESKQLTRQIEQYFDSRIEAIKIKVDAV
                     "
     gene            complement(1997..>2423)
                     /locus_tag="VKQ52_12405"
     CDS             complement(1997..>2423)
                     /locus_tag="VKQ52_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88044.1"
                     /translation="DNRTGGFIVRSKKAGSLNSEQQDVVNFLFGQGERIVIPEAETAS
                     HLASEEVELNGANFAIQTVAGKGSMSQQIADATNQAGNLVLNIRTRTDKNELKKGLAS
                     VKGRKGLNTVMVLYDNKRTVITKKEILNKDFKALDSLFN"
BASE COUNT          523 a          647 c          532 g          721 t
ORIGIN      
        1 tattctgtgt gacttcttca gtttcgcacc ggaggatgtc ctgcaatatt atattaatca
       61 tatatcctat atagcaacgg aggatgtttc attggataat ccgtatggcg cggccacctt
      121 cttttttatc aaatattcca cctaataaca atcccttata atcgaaataa ttatttgccc
      181 ttattgtaac tacaaggacc agtctggata tagtttatga tgatttttta aaatttatgc
      241 ttggggtttt acacggtacg cattgccgtg taaacaccta gccattaatc atattttaac
      301 tataccttat cgtttacagc cgtttactac ttcgcctatt tcttaaattt agtttagact
      361 catcattcct taacctctgt ctaaaccggt tacctatggt acacatccga cctacggaga
      421 tcatcgttac aatctctacc cgaaaccccg tggaggacct cttttgtttt caaaatgccc
      481 taatggcaat gatccagcac gttgaccctg tgcagttagg cggctcacaa gacccgctct
      541 acttctatgt ggaactgctg cgaaactttc tgccagatga gcgacaatgg aagacctttc
      601 taagagttta gtggcaaggc cccgtcaggg gccttttttt ttgcactgtg tcaaactttt
      661 ccttcttgct ttggtaaatt cttttaatat tgatccatgc tcggccagcg ttcccttttt
      721 gatagtatca tcgacgtgcc gcaggcccct aagaataaca ggggcggcag agacccgcag
      781 ctactccagc ggcggaatga agtgattgtt gcccggtatt attacctggt gcgcatcaaa
      841 aagatgcaat acccggaagc cctggccacc ctccgggtac aatttttcca ttccgaaatt
      901 atcatccagc gcgtcctcca gcggcattat gagcagttaa aacgattcaa ggaagaaagg
      961 ccgccggtcg gagtgtttaa aaagaattac ccctggctgg tttggtaacc cctcacgtga
     1021 aggctatgtc tatccccagg cccgtattac ccggcgctgg ctggtaagcc ggtgagcagg
     1081 tcatatcctt atacgtaaat tcatacgtaa ccgcgatcac tttgtaatca tgccttttct
     1141 ccgtttccct ccttgtccgg gtcagcggac caaagataga gccgcgccag ccgtgcagcg
     1201 cctggttgac ggcgatctcc gtgtcataca tactgacggc atattgccgt acctgttctt
     1261 tgaccaggtt attgcctggt tgccaattgg gaaaggccag ccgtatcttt agagtagctg
     1321 gttcggccag ttgcaccttg tttcccaggt tactgaactg cgcctcgtca aaatcgatca
     1381 gcgcacaggg aaagccaacc ggcgcacggg tcccctcttt gacattatcg atctgcccga
     1441 agtcctggtc gatgtatttg attgccggaa cttgtgattc gatccgctcc agtgtctcca
     1501 gataaatttc tttcataccg catctacttt tattttaatg gcttcaatcc ggctgtcgaa
     1561 atactgctcg atttgccggg taagctgctt actttctccc atgaacttcc gttgttgggc
     1621 cttatacata tacgcccgga taggcagctc tttgccggtt ttgcttttcc gcatgtgggc
     1681 tttcacctgg ataataccgc cctcgttatg gactttggcg tacttaatgt ccgagccgat
     1741 caccacctcc tggctacttt tccgaacgac ccggatgctc ttacgtagct gcccggtctt
     1801 aaacaggatg gccctgccct cgtccttgtc cttttcacgg ggaggccagg gctggagggt
     1861 atcatctacg aagccttccc tttggaaatt ctcgttaaaa tgattcacag ccagatcccc
     1921 aatgtagcgg ggagcgtccc gttggagttg cttcagctcc tgggcgagca ccttcattgc
     1981 ctgaatgatt cccatatcag ttaaataagc tgtccagggc tttaaagtcc ttgtttaata
     2041 tttctttttt ggtaatgacg gtccgcttgt tgtcatacag gaccatgacg gtattcagcc
     2101 cttttcggcc ctttacgctg gccagccctt tcttcaattc gtttttgtcg gtccgggtcc
     2161 gaatattcag caccaggttt ccggcctggt tcgtcgcgtc cgctatttgc tggctcatgc
     2221 tccctttgcc ggcaacggtc tggatggcga aattcgcccc gttcagttcc acttcctcac
     2281 tggccaggtg ggaagcggtt tccgcttccg gtatcacaat ccgttcgccc tggccgaaaa
     2341 gaaaattaac gacatcttgc tgctcactat taagactgcc tgctttcttg ctacgcacga
     2401 taaagccgcc cgttcgattg tcc
//
LOCUS       DATGFG010000666         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1535889, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000666 DATGFG010000000
VERSION     DATGFG010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535889"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2314)
                     /locus_tag="VKQ52_12410"
     CDS             complement(<1..2314)
                     /locus_tag="VKQ52_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HLZ88045.1"
                     /translation="MRIALSITLGLLLTLHLSALPTVKTGGLPAWLYTIHPDLDKKPA
                     RDEISDGYFYQLLDLQTSLPDKARYTHYIINITNESGVQNASEVSVTFAPEFQQVVFH
                     RVNIIRGGTVLNQLNLSRIRVIQEEKEAGEFEYNGLKRAFLTLKDVRKGDRIEVAYSV
                     VGFNPVFGNKYSDDFAFNSSTAVCNYYKSITTTQDRPLQVLSVNKAPAPVEQHLGNTL
                     VYSWDNPELQSSDPDSDTPSWFDKSPTIYVTEYKNWAEVIDWGMNTFNHYHFPLPAGL
                     QQKIAAWRDRAKGDKDLFANIATRFVQDEIRYLGLEIGVNTHKPHAPADVFNQRFGDC
                     KDKALLLTTILQQEGIPASVALISTDTRSQLAGIAPSPRDFDHAIVAIRRSKGVYTYV
                     DPTNSGQRGELADLYIPDYGYALVLEDGATGLQTVTPGRINDYTITEQLDARYYDSSR
                     FNVTSIYTGGAADDIRGMFAESSLRDVEDRYRKYYASFLEGVLPARPVTWHDDSTKNE
                     VTVQEHYTVPQLWSTDKKGKKYFEFTVRILDQAFPDPADATDDAPLALTYPYNVHYTL
                     NLSLPEDWDWGGGEMHIKNDAYQFDFIPTVHGSNMTLHYSFKSLRDHIAASDIRQYKE
                     DYKNIISKIAFQLYKTITPDGSLAEETPAEMKTTPSAPAIKDWKVCWPAIWLTFFFSL
                     LFSRLFAWLNSRGEETLYAPGSGYPLGGWIVLLGISIGINLVLEGTQLFRENSYSYSN
                     WTAFGNAGGTNLQYLFMTELAIHLSFLAGGG"
     gene            2703..>3576
                     /locus_tag="VKQ52_12415"
     CDS             2703..>3576
                     /locus_tag="VKQ52_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88046.1"
                     /translation="MTITLHTYLLLSCSSLFSQQVSYVEPYSDRFTLRQEMLGKVGDY
                     YWVSAITRKRAVRRSEFASTEERNFMIYDGRMSLKSLLDDPPYTVNSVREYLVAADDH
                     FDRVNLLNAGSRETEVRLQRYEPGGEALEPDHTIGDFPFAEPGTSFMLVRSQDRARIL
                     LLGFEFLSSSPPRLHAILYDQDWHLLSQRVYKHPFLTQPIIQDDYSTFPLEDFNKSPI
                     KLADDGQWLMLSPSRLNSNFLLFHFSPVDTAVSCREIMLPGTSSMEDVCLSIDNTTGE
                     AVGGVLSTFHYSPLK"
BASE COUNT          748 a          790 c         1139 g          899 t
ORIGIN      
        1 cgccgccgcc ggcgaggaag ctgaggtgga tggcgagttc cgtcatgaag agatattgga
       61 ggtttgtgcc gccggcgttc ccgaaggcgg tccagttgct atagctatag gagttttcgc
      121 ggaacaactg ggtgccttcc agaaccaggt tgatgccgat gcttatgccg agcaggacga
      181 tccagccgcc cagtgggtat ccggagcccg gcgcataaag ggtttcttcg ccgcggctgt
      241 tcagccaggc gaagaggcgg gagaagagga gggaaaagaa gaaggtgagc cagatggcgg
      301 gccagcagac cttccagtcc tttattgccg gtgcggaggg ggtggttttc atttcggcgg
      361 gtgtttcttc cgccagggag ccatccgggg tgatcgtctt gtagagctgg aaggcgatct
      421 tactgatgat gttcttatag tcttccttat actgccggat gtcggatgcg gcgatatggt
      481 cgcgaaggga tttgaatgag tagtgcaggg tcatattgct tccatgcacg gtggggatga
      541 agtcgaactg ataggcgtcg ttctttatgt gcatttctcc gccgccccag tcccagtctt
      601 ccggcaggga gaggttcagg gtatagtgaa cattgtaggg ataggtcagc gccagcgggg
      661 cgtcgtcggt ggcgtccgcg gggtcgggga aggcctgatc caagatcctg acggtgaact
      721 cgaaatattt tttgcctttt ttgtcggtgc tccagagttg ggggacggta tagtgttcct
      781 gtaccgtcac ctcatttttt gtgctatcgt cgtgccaggt gacgggcctg gcgggcagga
      841 cgccttccag gaaggaagca taatatttgc ggtaacgatc ttcgacgtcc ctgaggctgc
      901 tttctgcgaa catgccacgg atgtcatcgg ctgcgccacc ggtgtagatg gaggtgacgt
      961 tgaaccggga gctgtcgtag tatctggcgt ccagctgttc ggtgatggtg tagtcgttta
     1021 tgcggccggg agtgacggtt tggagaccgg tggccccatc ttccaggacc agggcgtaac
     1081 cgtagtcggg gatatagagg tcggcgagtt cgccccgttg cccggaattg gtcgggtcga
     1141 cataggtgta tacgcctttt gaccgccgga tggccacgat ggcgtggtcg aagtcgcggg
     1201 gggagggggc gataccggcc agctggcttc gggtgtcggt gctgatgagg gccacggatg
     1261 cggggatgcc ttcctgttga aggatggtcg tgagcaggag ggccttatcc ttacagtcgc
     1321 cgaaccgctg gttgaaaacg tctgcggggg catggggttt gtgggtattc acgccgatct
     1381 cgaggcccag gtaacggatc tcgtcctgta cgaagcgggt ggcgatgttg gcgaagaggt
     1441 ccttgtctcc ttttgcgcgg tcgcgccagg cggcgatctt ttgttggagg ccggccggca
     1501 ggggaaaatg ataatggtta aaagtattca tgccccaatc gatgacttct gcccaatttt
     1561 tgtattcagt aacgtagatg gtgggggatt tgtcaaacca ggacggggta tccgaatcgg
     1621 ggtcggagga ttgcagttcc gggttgtccc aggaatagac gagggtgtta ccgaggtgtt
     1681 gttctacggg cgccggcgct ttgttcaccg acaggacctg cagggggcga tcctgggtgg
     1741 tggtgatcga cttataataa ttacataccg ctgtggaact attgaaggcg aagtcatccg
     1801 aatatttatt tccgaaaacg ggattaaagc cgacgacgga ataagctact tcgatgcggt
     1861 cgcctttgcg gacgtctttc agggtgagaa aggccctttt cagaccgttg tattcgaact
     1921 caccggcttc tttctcttcc tggatgaccc tgatgcggga gaggttcagc tgattgagga
     1981 ctgtgccgcc gcggatgata tttaccctgt gaaagacgac ctgctggaat tcgggggcga
     2041 aggtaacgga gacctcggag gcgttctgga cgccggattc attggtaata ttgatgatat
     2101 aatgggtgta gcgggcttta tcggggaggc ttgtctggag atcgaggagt tgataaaaat
     2161 agccgtcgct tatttcgtcc cgggcgggtt tcttatcgag atcgggatgt atcgtgtata
     2221 gccaggcggg aaggccgccg gtttttacag tgggcagggc ggagaggtgc agggtcagga
     2281 gtaagcccaa tgtgatggac aatgcgatac gcatgaataa ggatcattag gttttcaaac
     2341 ttacaaaaaa aaagggccag gatagaaatc cagccccgtt tttaaatcca atatcctatg
     2401 aaaaaccgaa ttatataggg caaccggcgt gccggaaggt gcgcgagatc caagggaggt
     2461 aaaaaacttt tttagactgc tgatttatag ttacttatat ttcttgatga gttctaatgc
     2521 gaaatcacgc ctatacacgc ttggcttatt tcctcccagg aaaggaaaat ttttcccatt
     2581 gcaggaatga tgcggggtgg catggggaac atggccctta gcggtggggt aatcaaatga
     2641 tcatactgat gcattaaaaa aattgttaac ttcaagaaga aggagttggt atgggatggc
     2701 gcatgacgat aacactacac acttatttat tattaagctg ttcttcgctc ttttcccagc
     2761 aggtgtcgta tgttgagccg tatagtgacc ggttcacgtt gcgacaggag atgctgggta
     2821 aagtagggga ttattattgg gtgtcggcga taacgcgtaa gagggcggtg cggcgcagcg
     2881 agtttgcgag cacggaagaa cgtaatttca tgatctacga tgggcggatg agtttgaaga
     2941 gtctgctcga cgatccgcct tatacggtga attccgtccg ggaatacctg gtggcggcgg
     3001 atgatcattt cgatcgggtc aaccttttga atgccggcag cagggaaacg gaggtccgtt
     3061 tgcagcgata cgagcctggc ggagaggcgc tggaacccga tcatacgatc ggtgattttc
     3121 cctttgctga acccgggacc agttttatgc tggtgcgcag ccaggacagg gcgcggatcc
     3181 tgttgttggg ctttgaattc ctttcttctt ctcctccccg tctgcatgcc atattgtatg
     3241 accaggactg gcatctgctt tcccagcggg tgtacaagca tcctttcctg acgcagccga
     3301 tcatccagga cgattattcc acctttccgt tggaggactt caataaatcg ccgatcaagc
     3361 tggccgatga tgggcagtgg ctgatgttat ctccttcacg gctgaacagc aatttcctgc
     3421 ttttccattt ctccccggtg gatacggcgg tctcctgccg ggagatcatg ttacccggga
     3481 cctcttcgat ggaggatgtc tgtttgtcga tagataatac tacgggggaa gccgttggag
     3541 gggtgttgtc gaccttccat tattcaccgc tgaaga
//
LOCUS       DATGFG010000667         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1576365, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000667 DATGFG010000000
VERSION     DATGFG010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576365"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1597
                     /locus_tag="VKQ52_12420"
     CDS             95..1597
                     /locus_tag="VKQ52_12420"
                     /inference="COORDINATES: protein motif:HMM:NF042600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6770 family protein"
                     /protein_id="HLZ88047.1"
                     /translation="MKKLTSIISLLLFACSLQAQSKIFKEVGEDIATQMKTITQDNAL
                     VGYLAFTRLEKADADSFNYRVTIMDENLNDIGTVNFRQGYLDLQAVSFEQNVLCLGYV
                     QSPLASGESVRTRKDYKKAQDAAMSSHILVQFINLGGKVINTYYKEVTLRMETLTGKS
                     AFSAMKIIGYLKYGMQIRNIPNRGFALFYGDDVKQNLLLFDTRGALTHEQEIPTIADR
                     YYLRTTATDIYLLLKDNVRVPEGGFKLYLYSAKDLTAENYFDLRDGYDNWLKVLSFDN
                     DPATGDAFIAGCIINPHRERQFVTPTDYSYTPYLGLFTLDLGNPHKEMHANCSYWSSE
                     TIPGISKDGLFTDKDFYVKYATAFRDYNGNTIFAGTALVGKGFVGAAKYKLTDGVFVR
                     QEASGNIALDNTIPCDETKYFGPAAILYDLDKKDFYKVVNQDTKSNYMIIDDEENIYI
                     YNVNGKKVVRTIPHKDGNVKINVYPAKEGHMMVSEYNRKEKYTRFSIEAL"
     gene            1657..2172
                     /locus_tag="VKQ52_12425"
     CDS             1657..2172
                     /locus_tag="VKQ52_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HLZ88048.1"
                     /translation="MITGITFIEAFVEDPGNLYTFLRHTANWDERMTARMTASYGKAY
                     NYSQMEYPFRPLLPELKQLAHKIGEQIGFVPNNCLINFYTDGKSKMGFHSDQTDILEA
                     GTGVAIVSLGETRTLKFRNIADPSQTESYELPSGSLIYMTQEVQSLWQHAVPKADTDR
                     GRMSLTFRQMK"
     gene            complement(2189..2533)
                     /locus_tag="VKQ52_12430"
     CDS             complement(2189..2533)
                     /locus_tag="VKQ52_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88049.1"
                     /translation="MRKLIMKMPISIDGFVSDANGKNDWVFKTGDEESLAWSVGAVRQ
                     AGLIIMGRKSFEGLAPYWPTAPGPFAAPGKPIVAIGGAGFMRSLILTSHSKNLPSGVR
                     HIVSIPRQARYA"
     gene            complement(2554..>2756)
                     /locus_tag="VKQ52_12435"
     CDS             complement(2554..>2756)
                     /locus_tag="VKQ52_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88050.1"
                     /translation="NGNPRNNAFSLVNNSFKPGAETTTVYIEKVFDDLATLEFMVSGG
                     YKEGLAAGMSNLDEYFRTLVTK"
BASE COUNT          734 a          667 c          686 g          669 t
ORIGIN      
        1 aatatatcgg taattatgcc acgagcatgt attgtgaatt tgtcgatcgc ttcagaaaaa
       61 aatgaacagg aacctttaac aataagacat cgtcatgaag aaattaactt caataataag
      121 cctgctgctt tttgcctgtt ccctgcaggc tcagtcaaag atcttcaaag aggtaggcga
      181 ggatatagcg acccagatga agaccatcac ccaggacaat gccctggtcg gttatctggc
      241 cttcacccgg ttggaaaaag ccgatgcgga ctcgttcaac taccgcgtaa cgatcatgga
      301 cgagaacctc aatgatatcg gcacggtaaa ttttcggcag ggatacctcg atctccaggc
      361 cgtatccttc gagcagaacg tgttatgcct ggggtatgtc caaagcccgc tggcgagcgg
      421 agaatcggtc aggacccgga aagattacaa aaaggcgcag gatgccgcca tgtccagcca
      481 tatcctggta caattcatca accttggcgg caaggtaatc aacacctact ataaggaggt
      541 aaccctgagg atggagacgc ttaccggcaa aagtgctttt tcggcgatga aaataatagg
      601 gtatctgaaa tatgggatgc agataaggaa tatccccaat agaggtttcg ccttgtttta
      661 tggcgacgat gtgaaacaga acctgctatt gttcgacacc aggggagccc tgacccatga
      721 acaggagatc ccgaccatcg ccgatcgtta ttacctgcgt actacggcga cggatatcta
      781 tcttttgctg aaggataatg tacgcgttcc cgaaggcggg ttcaagctct acctatattc
      841 cgcgaaggat ctcaccgccg agaattattt cgatctcagg gacgggtatg acaactggtt
      901 aaaagtcttg tcgtttgaca acgatcccgc cacgggcgac gcgttcatag ccggctgtat
      961 cattaacccg caccgggagc ggcagttcgt cacgccaacc gactattcct atactcctta
     1021 cctgggttta tttacgctcg atctgggaaa tccgcacaag gagatgcatg ccaattgcag
     1081 ttattggtcc agtgaaacca ttcccggtat ttccaaggat ggcctgttca ccgacaagga
     1141 cttttacgtc aaatatgcca cggcattccg ggattataat ggcaatacca tttttgccgg
     1201 cactgccctg gtagggaagg ggttcgtggg agccgccaaa tacaagctga cggacggggt
     1261 cttcgtgcga caggaggcct ccggcaacat cgccctggac aatactattc cctgtgatga
     1321 gacaaaatat ttcggccccg cagccattct gtacgacctg gacaagaagg atttttacaa
     1381 agtggtcaat caggatacta aaagcaacta catgatcatc gacgatgaag agaatatcta
     1441 tatctacaac gtgaacggca aaaaggtggt gcggacgatc ccgcacaagg acggcaatgt
     1501 aaagatcaat gtctatcctg ccaaggaagg gcacatgatg gtgtcggaat acaaccggaa
     1561 ggaaaagtac acccgctttt cgatcgaggc tctttaaagc ggccggagca gtgcatataa
     1621 taaaaacaga cggaattgtt attatcttaa attcacatga ttaccggaat aacctttata
     1681 gaggcgttcg tcgaagatcc cggaaattta tatacttttc tgaggcacac ggcaaattgg
     1741 gatgaaagga tgacggctcg catgacggca agctatggca aggcgtataa ttattctcaa
     1801 atggaatacc ctttccggcc tttgctgccc gagttgaaac aacttgctca caagataggg
     1861 gagcagattg gcttcgtgcc aaacaattgc cttatcaact tctataccga tgggaagtcc
     1921 aagatgggtt ttcattcgga ccagacggat atcctggaag ccggtacggg tgttgcaatt
     1981 gtttctcttg gagagacgag aacactaaag tttcggaata ttgctgatcc ctcccaaacc
     2041 gagagttatg aattgccttc cgggagcctg atctacatga cgcaggaagt gcaatcactc
     2101 tggcaacatg cggttcccaa ggcggatact gatcggggga gaatgagttt gacgtttcgg
     2161 cagatgaaat aaaatgggcg aaatatgttt atgcgtacct cgcctgcctt gggatcgaca
     2221 caatatgcct tacgccggat ggtaagtttt tgctatggct cgttaggatg aggctccgca
     2281 taaatcccgc gccaccaatt gctacgatcg gtttccccgg cgcggcaaaa ggcccgggcg
     2341 ccgttggcca gtaaggggcc aatccctcaa acgacttcct gcccatgata atcaatcctg
     2401 cctgccggac tgccccaaca ctccaggcca gcgattcctc gtctccggtc ttgaagaccc
     2461 aatcgttctt gccattggcg tcgcttacaa aaccgtcgat cgagatcggc atcttcatga
     2521 tcaattttcg cataacgaaa ggtttgcttt ttattatttg gtgaccaggg tgcggaaata
     2581 ttcgtccaga ttgctcattc ccgcggcaag gccttccttg taccctcctg agaccatgaa
     2641 ttcaagggtg gcgaggtcgt cgaatacttt ttctatgtac accgtggttg tctctgcccc
     2701 tggtttaaag gagttattca ccagggaaaa ggcattgttc ctgggattgc cgtttt
//
LOCUS       DATGFG010000668         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_64177, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000668 DATGFG010000000
VERSION     DATGFG010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64177"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3010)
                     /locus_tag="VKQ52_12440"
     CDS             complement(<1..3010)
                     /locus_tag="VKQ52_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ88051.1"
                     /translation="MRKLLRILGCAIALVWSLQTVAQDQAITGVVRDPKDGAPLSGTT
                     IVNKRTTKSVMAKEDGTFTIQARPGDKLMLSHIGRKTNTITIGSQSNYTIALEPENSD
                     LGEAVVTAMDIKRNPRELGYSVQSVTGKTIAETQRENFITALQGRVSGLTITSTGGQA
                     GASASIVLRGFNTLGGTNQPLFVVDGIIIDNTTFNQNSYGGTGVGLSQDLPNRNNDYT
                     NRIADLNPNDIESLTVLKGPEATALYGSQASNGAIVIVTRKPKAGAKPTFVYDDNFRA
                     QTITRFIDVNNSYSSGYTNGVPSVPTSTQLLRYFGPAWTGGTPLYDNLHHFFKSAFSQ
                     THNLAAEFGVRNSGFRISGSYFNSDGVVPHNNYLKSNIRIVNTTRIGKYVDINPSIEY
                     IHSNNLKPQKGGSSYLEDLYLWPANNDIRNYQDKNGNKQVLYQTNVNADYDNPLWSAK
                     NNKTRDITDRVIATLGININPFSWLSIAGRFGYDTYEQDGYLFTHPESFLLTSATGGT
                     LDNYYHRYSGYNHTITATARKKVGDFSLRLLGGTQWQDLETKAWDVNGTHLIDSARTD
                     SNNTLPSSRIRLLRNQQGHSNLQITREIAYFGEFTLGWRDLIYLTYSHRFESASPLPA
                     QNRNYNYPAASLSAIMSDIIPSIKSNNILNYWKIRGSFAGTARLNDPYSNQSFFVNNL
                     ATQYIPSFAYGYTNSNPYLRPERQRTFELGTEIRLLNNMINIEGAYYNTYCYDQIAQI
                     YRASYATGFILNTANAASTRNEGIELTVGVSPVKHKDFSWDIQFNFNHMWSNVLTLPN
                     SIGQLNDYYNSDTYFEGNVRAGLIRGHSTGTITGFNYLKNNAGQVLIDPGSGVAIRQQ
                     LNYSIGDRTPKFTLGTNNVFRYKNWNLNFLWDLKVGGDIYNGTDYFLTGIGKSARTNA
                     RNTPVVVKGVLQDGNENTSHPTVNNISIIPYFNTFYYTNLPDVEFVQRNVNWLRLRDI
                     TLSYRMPETIFKHVRGVKGLLVFATANNLIL"
     gene            complement(3141..3890)
                     /locus_tag="VKQ52_12445"
     CDS             complement(3141..3890)
                     /locus_tag="VKQ52_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HLZ88052.1"
                     /translation="MLKQERQAYILHQVNLHNQVLSTSLSQELDVSEDTIRRDLQDLA
                     DSQKLIKVHGGALSLSYSDYRHPANPIYAHAQKKLIARKAAALIQNDMFITTTGGTTL
                     IELARHLPQNLKATFISGSLPVTLEYCHHPLIDVILIGDKVSKNARITVGGEAIARIR
                     QIKADICFLGINAIDVQHGITDNDWEVVQVKKAMIESAKKVICLTISEKINSFQPFHV
                     CPVEDLDMLITELPPDDPLLEPFRQKGIEIV"
BASE COUNT          832 a          893 c         1400 g         1086 t
ORIGIN      
        1 gcaggatgag gttgttggcc gttgcaaaga cgagcaaccc tttgaccccg cggacatgtt
       61 taaaaatggt ttccggcatc cggtagctga gcgtgatgtc ccgcagccgc aaccaattga
      121 cattgcgctg aacgaattcc acgtccggca gattagtgta atagaaggta ttgaaatacg
      181 ggatgatcga gatgttgtta acggtgggat gcgaagtatt ttcatttccg tcctggagca
      241 cacctttgac gacgacggga gtattcctgg cgttcgtcct tgcgcttttt ccaatacccg
      301 tgaggaaata gtcggtaccg ttatagatat cgccgccgac cttcagatcc cagaggaaat
      361 tcaaattcca gttcttatac cggaagacat tgttggtccc cagggtgaac ttcggagtgc
      421 ggtcgccgat ggaatagttc agctgctggc ggatggcgac gccgctaccc ggatcgatca
      481 acacctgccc ggcgttgttc ttcagatagt tgaagcccgt gatggtgccg gtggaatgtc
      541 cgcggatcaa gccggcgcgg acgttgcctt cgaagtaggt gtcggagttg tagtagtcgt
      601 tcagttgacc gatggagttg ggaagggtca gcacattgct ccacatatga ttgaagttaa
      661 actggatatc ccaggagaaa tccttgtgtt tgaccggcga aacacctacg gtgagctcga
      721 tgccttcgtt ccgggtggag gctgcattcg ccgtgttgag aatgaagcct gtggcgtagc
      781 tggcccggta gatctgggct atctggtcat aacaatacgt gttgtagtag gccccttcga
      841 tgtttatcat attgttgagc aggcggatct cggtacccaa ttcgaaggtg cgctgtcttt
      901 ccggccggag ataggggttg gagttggtgt agccgtaggc aaaggatggg atatactggg
      961 ttgccaggtt attgacgaag aaggactggt tggaataggg gtcattcagg cgggcggtgc
     1021 cggcgaacga accacggatc ttccagtagt tgaggatatt gttgctcttt atcgatggga
     1081 tgatgtcgga catgatcgcg ctgaggctgg ctgccgggta gttatagttc ctgttctgcg
     1141 caggcagcgg agaagcgctt tcaaaccggt gggaataggt caggtagatg agatcgcgcc
     1201 agccgagggt gaactccccg aagtaagcga tctcacgggt gatctgcagg ttggaatgtc
     1261 cctgctggtt ccggaggagc ctgatccggc tgctgggcag ggtattgttg ctgtcggtgc
     1321 gggcggaatc gatgagatgc gttccgttga cgtcccaggc cttggtctcg agatcctgcc
     1381 attgcgttcc gccgaggagc cggagcgaga aatccccaac ctttttcctg gcagtggcgg
     1441 tgatggtgtg gttataaccg ctgtagcggt ggtaataatt atcgagggta ccgccggtag
     1501 cggaagtgag gagaaaggat tccggatggg taaaaagata accgtcctgt tcataggtgt
     1561 cgtagccaaa acggccggcg atggaaagcc aggagaaggg attgatattg atccccaggg
     1621 tggcgatcac ccggtcggtg atatcgcggg tcttgttgtt cttggcgctc cagagtgggt
     1681 tgtcgtagtc ggcattcaca tttgtctggt aaagcacctg tttattacca tttttgtcct
     1741 gatagttccg gatatcgttg ttggccggcc ataggtaaag gtcttcgaga tagctggaac
     1801 cgcctttttg gggcttgagg ttgttggagt ggatgtattc gatcgacggg ttgatatcta
     1861 catatttacc gatcctggtg gtgttgacaa tacggatatt ggacttcagg tagttgttgt
     1921 gggggacgac gccgtcagag ttgaagtagg acccactgat ccggaagccg ctgttcctga
     1981 ccccaaactc tgccgcaaga ttgtgggtct gggaaaaggc ggacttaaag aaatggtgca
     2041 ggttatcata cagtggggtg ccgccggtcc atgcaggccc gaaatacctc aacaactgcg
     2101 tgcttgtcgg gacactcggt acgccattgg tatagccact gctataggag ttgttgacgt
     2161 cgataaaacg ggtgatcgtt tgcgcgcgga aattgtcgtc atagacaaag gtgggctttg
     2221 cgcctgcctt gggcttccgc gtaacgatca cgatggcgcc gttggaagcc tggctgccgt
     2281 aaagggcggt ggcttccggt cctttcagaa cggtaaggga ctcgatatca ttcgggttaa
     2341 ggtccgcgat ccggttggta tagtcattat tccggttggg taaatcctgt gacagcccca
     2401 cgcccgtgcc gccatacgag ttctggttaa aagttgtatt gtcaataatg atcccgtcta
     2461 cgacgaacag cggttggttg gtgccgccaa gcgtattgaa accccgcaac acgatggatg
     2521 cggaagctcc cgcctgtccg ccggtggatg taatcgtcaa accggatacc cggccctgga
     2581 gcgccgtgat aaaattctcc cgctgggtct cggcgatggt cttgccggtc accgactgga
     2641 cggagtagcc caattccctg ggattgcgtt tgatgtccat agcggtgacg accgcttcgc
     2701 ccaaatcgct gttctccggt tccaatgcga tggtgtagtt ggattgacta ccgatggtaa
     2761 tcgtattggt cttgcggccg atatgggaga gcatcagttt gtctccgggg cgggcctgga
     2821 tcgtgaacgt tccgtcttct ttcgccatga cggatttcgt cgttcgcttg ttgacgatgg
     2881 ttgtccctga caagggagcc ccatctttag ggtcgcgcac cacaccggtg atcgcctgat
     2941 cctgtgcaac ggtctgcagg ctccagacca aggcgatggc gcacccgaga attcgtaaca
     3001 attttcgcat ggttagttct gttttttgtt gagtttggtt atttgaatag cgcacatggc
     3061 gaacatgttc gtccttttca aagggaggct tatgtagggt gggatggtgt gatcagggct
     3121 agcgggatcg gcggataggt ttagactatt tcgatgcctt tttggcgaaa gggttccagc
     3181 agggggtcgt ccggcggcag ttcggtgatc agcatatcga ggtcttcgac ggggcagaca
     3241 tgaaagggtt ggaaggagtt gatcttctcg gaaatggtga gacagatgac cttctttgcg
     3301 gattcgatca tggctttttt tacctggacc acttcccagt cgttgtcggt gatgccgtgc
     3361 tggacgtcga tggcgttgat gccgaggaag cagatatcgg cctttatctg gcggatgcgg
     3421 gcgatggctt cgccgcccac ggtgatgcgg gcgtttttcg acactttgtc gccgatgagg
     3481 atcacgtcga tgagggggtg gtggcaatat tccagggtta cgggcaggct gccggagatg
     3541 aaggtggcct tcaggttttg gggcaggtgc cgggccagtt cgatgagggt ggtgccgccg
     3601 gttgtggtga tgaacatgtc gttctggata agggctgcgg ctttgcgggc gatgagtttt
     3661 ttctgcgcat gagcatagat ggggttagcg gggtggcggt agtcggaata ggagagggat
     3721 aaggcgccgc cgtgtacttt tatgagtttc tggctgtcgg ccaggtcctg gagatcgcgg
     3781 cggatggtgt cttcggatac gtccagttcc tggcttaggc tggtggatag gacctggtta
     3841 tgaaggttca cctggtgtaa gatatacgcc tgtctctcct gcttgagcat ggtcgttttg
     3901 gttgagtttt agtagctgcg gcctaaagta agctaaaaaa ccgcaaaaaa acgcaagcgt
     3961 ggattaaaat gtgcaaaaaa acgcagagaa ggtgcgggat aatgcgggag tgtgcgagtg
     4021 ttgtggggat gttgagggag tgtgcgggtt tgcgggggcg gggaggtttg ggtggtggga
     4081 ggtttgggtt ggctggagtc ggtggtggtg ggaggctttg gttgggggag gtttgggggc
     4141 tgtgggtagt gggtttgggg ttgttggtgg agggaggttt ggtagtggga ggtttgggtt
     4201 ggagcagttg a
//
LOCUS       DATGFG010000669         5121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_324327, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000669 DATGFG010000000
VERSION     DATGFG010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324327"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2466
                     /locus_tag="VKQ52_12450"
     CDS             <1..2466
                     /locus_tag="VKQ52_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HLZ88053.1"
                     /translation="KRPIRYLIRIAGALVCLLLLAWLCLVGYVLLNKASLLEKARSAL
                     QERVGGEVRIGKLELSLFRHFPNITARFSDVSMRDSAWQQHHHDLLKVADVYVSCNLF
                     RTVLTRQVQPGEIDLEHGQVYFYTDSTGYTNTYMLSDRKTDKPRKAADPPDIGLTDIR
                     LVVERQDKRKLFDLDVWRLRCTIEKDNRLLHLTVNEEILVNQFAFNTEKGSFIKGKRL
                     SGQFGVDYNTASKIIQFTDARVAIDDHPFLFSGRFFPTVVPDPFILKIGTDKILYRQL
                     TALLTPNIQQKLDQYDIDKPVTIAAQLDAGSADDHEPQIQVRLNLDNGSVLTPTGRFT
                     AVSFKGSFTNEWLRGQKRGDENSGIRFLGFSGKLQNLPLRADTVTITNLNHPQLACDL
                     HSQFALSLLNDITGSQTLQFTGGSGTMNLLYKGPLSENDTAGTVVNGRLDLDSAALIY
                     LPYQFRLTNGKGRLLFKDQDLFIEQVNIRAGGTRITVRGVARNLVALLDRNAENVSMD
                     WSLTTPHLDLEDLTVLAGRPVAATVRRNDNNSLFGAAFSRVDRLLKEGTIHVGLEAAD
                     LRFRKFTGAHAKADLVFDDHEIKLTRLNVEQGGGSLSLKATLDRKGPGEGNPVTLESH
                     LDQVDLPKLFTSFNDFGQDAIGARNLRGKLTAEIRLAGALTNKAKMVGNSLKGTIGFT
                     IRDGQLVDFAPMEKIQMAVLKNKGLSEIRFGELTNQLDLDSTTLTIHRMEIRSTAFTL
                     FAEGVYDLRKGADMSLQIPLSNLSRNRNPEIPPDSRGNDSKAGLSVRLRARTGEDGKL
                     KVSWDPFKKALKKVKPHSGRGGR"
     gene            2505..4544
                     /gene="uvrB"
                     /locus_tag="VKQ52_12455"
     CDS             2505..4544
                     /gene="uvrB"
                     /locus_tag="VKQ52_12455"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HLZ88054.1"
                     /translation="MPFNLNAPFPPSGDQPEAIRQLTEGLLTGEQYQTLLGVTGSGKT
                     FTIANVIQNVQRPTLVLTHNKTLVAQLYGEFRQFFPDNAVGYFVSYYDYYQPEAYMPV
                     SNTYIEKDLSINEELEKLRLQATTQLLSGRRDIIVVASVSCIYGIGNPAEFENGIVRL
                     QQGQVISRQGFLHSLVNSLYKRSEIEFARGNFRVKGDTVDINLPYVDYGYRISFFGDE
                     IDSIESFDIQTGKRIAALDNAAIFPANLYLAPKDILHQVIYEIQDELAEQVTYFKNVG
                     KYIEAQRLSERVNYDVEMIRELGYCNGVENYSRFFDRRMPGTRPFCLLDYFPKDFLLV
                     LDESHQTVPQISGMYGGDRSRKLVLVDYGFRLPSALDNRPLNFHEFESLTGQTIYVSA
                     TPGDYELEKTEGVVVEQVVRPTGLLDPPIEVRPSANQIDDLLDEIDKRVKKGDRVLVT
                     TLTKRMAEEMDKYLHRIDIKSKYIHSEVDTLERVEILRQLRLGEIDVLVGVNLLREGL
                     DLPEVSLVAILDANKEGFLRNEKSLTQTAGRAARNVDGMVIFYADTITVSMQRTMDET
                     LRRREKQIAHNEKHGIIPRTVIKSKEQVFAQTSVLDIKGYDPADKYALAPDEELVAVA
                     AEEQEVYQTIPQVEKAIAKTKKEMEKAARDLDFMEAARLRDEMFGLQKKLKEMKG"
     gene            4564..>5121
                     /locus_tag="VKQ52_12460"
     CDS             4564..>5121
                     /locus_tag="VKQ52_12460"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88055.1"
                     /translation="MENTDKRLFLLDALALVFRAYYALISNPRLTTKGRNTNAQFGFT
                     NALVDLINNQQPSHMAVCFDTEAPTERHTDFADYKANRQEAPEDLLSALPDIKAIIKG
                     FNIPCIEFDGYEADDVIGTLSKQAAAAGYEVYMVTPDKDYGQLVSEKIRIYKPGYQGG
                     TAEILGPKEVCEKWNIKDVAQVIDIL"
BASE COUNT         1167 a         1352 c         1544 g         1058 t
ORIGIN      
        1 aaacgaccca tacgatatct gatccgaata gcaggcgccc ttgtttgcct gctattgctt
       61 gcctggctgt gcctggtggg ttatgtcctg ttgaataagg cctcgctgct ggaaaaggcg
      121 aggagcgcct tgcaggaaag ggttggcgga gaggtccgga tcgggaagct ggagctgtcg
      181 ttgttccgcc attttcccaa tatcaccgcc cggttctcgg atgtgtcgat gcgggacagc
      241 gcgtggcagc aacaccatca tgatctgctg aaggtggccg atgtgtatgt ctcctgcaac
      301 ctgttcagga ctgtgctcac gcgccaggtg cagccagggg agatcgatct cgagcatggc
      361 caggtgtatt tctataccga cagcacgggg tatacgaaca cctatatgct tagcgacagg
      421 aaaacggata agccccgtaa ggccgccgat ccgccggata tcgggcttac cgacatccgc
      481 ttggtggtgg aacgacagga caaacgaaag ctcttcgatc tcgatgtctg gcggctgcgc
      541 tgtaccattg aaaaggataa ccggctgctc cacctgaccg tcaatgagga gatcctggtc
      601 aaccagtttg ccttcaatac ggagaagggc agttttataa aggggaagcg gctgtccggg
      661 cagtttggcg tggattataa tacggccagc aagatcatcc agtttactga tgcgagggta
      721 gcgatcgacg atcatccctt tttattcagc ggccgtttct ttcccaccgt ggtccccgac
      781 cctttcatcc tgaagatcgg gacggacaag atcctctacc gccagttaac ggccctgctg
      841 acgcccaata tccagcagaa gctggaccag tatgatatcg acaagccggt gacgatcgcc
      901 gcccagctgg atgccgggtc ggccgatgac catgagccgc agatacaggt gcggctgaac
      961 ctcgataatg ggagtgtcct cacgccaacg ggtcgtttta cggcagtatc cttcaaaggc
     1021 agttttacca acgagtggct tcgcggtcag aagcgggggg atgagaattc ggggatccgt
     1081 ttcctgggtt tttccggcaa gttgcagaac ctgccccttc gggccgacac cgttacgatc
     1141 accaacctga atcatccgca gctggcctgc gatctgcatt cccagttcgc cctctccctg
     1201 ctgaacgaca tcaccggcag ccagaccctt caattcaccg gtggcagcgg gaccatgaac
     1261 ctcctgtata aggggccgtt gtccgagaat gacacggcgg gtacggttgt caatggccgg
     1321 ctcgatctcg actccgccgc gcttatctat cttccttacc agttcaggct caccaatggc
     1381 aaggggcggc tcctctttaa agaccaggac ctttttatcg aacaggtcaa tatacgggcc
     1441 ggcggcacga ggatcacggt gaggggagtc gccaggaacc tcgtcgccct gctggacagg
     1501 aacgcggaga atgtaagcat ggactggagc ctgaccactc cccatctcga cctggaagat
     1561 cttacggtcc tggcgggacg tcccgtggcg gcaaccgtcc ggaggaacga caacaacagc
     1621 ctgtttggcg cggctttttc gagggtggac cgcctgttga aggaaggaac gatccatgtg
     1681 gggctcgagg ccgccgatct ccggttcaga aaattcaccg gcgcccatgc aaaggccgat
     1741 ctggtcttcg atgatcatga gataaaactg acccggctga acgtggagca gggcgggggc
     1801 tctctcagtc tgaaagccac gctcgaccgc aaggggccag gtgaaggtaa cccggtcacc
     1861 ctggaatccc atctggacca ggtagatctg ccgaagctgt ttacctcctt taatgatttt
     1921 ggccaggatg ccatcggcgc ccgcaacctg cggggaaagc tcacggcgga gatccggctt
     1981 gcgggggcgc tgaccaataa ggcaaagatg gtcggcaaca gcctgaaggg aacgatcggt
     2041 tttaccatcc gcgatgggca gctggtggac tttgccccga tggaaaagat ccagatggcg
     2101 gtcctgaaga acaaggggct ttccgagatc cggtttggag agttaacgaa ccagttggac
     2161 ctggactcaa caaccctgac gatccaccgg atggagatcc ggtccacggc ctttacgctc
     2221 ttcgccgaag gggtctacga tctcaggaag ggggccgata tgagcctcca gatcccgctc
     2281 agcaacctct ccaggaaccg gaacccggag atcccgccgg acagccgggg caatgacagc
     2341 aaggcggggc taagcgtgcg cctgcgcgcc cggaccgggg aggatgggaa gctgaaggtc
     2401 agctgggacc cgttcaaaaa ggcgcttaaa aaggtaaaac cgcattccgg ccggggaggc
     2461 cgatagccaa tccaggataa tcggtaattc ggtaatttcg ctctatgcca tttaatctta
     2521 acgcaccttt tcctccttcc ggagaccagc cggaagcgat ccgtcagctg acggagggtc
     2581 tccttaccgg ggaacaatat caaaccttat tgggggtcac cggctccgga aagaccttta
     2641 ccattgccaa tgttatccag aatgtccagc ggccgaccct tgtgctgacg cataacaaga
     2701 cccttgtcgc gcagttgtat ggggaattcc ggcagttctt cccggacaat gccgttggct
     2761 atttcgtatc gtactacgat tactatcagc cggaggccta tatgcctgtg agcaatactt
     2821 atatcgagaa ggacctgtcc atcaacgagg agctggaaaa gttgcggttg caggcgacga
     2881 cccagctgct cagcgggcgc agggatatca tcgtcgtcgc ttccgtcagc tgcatttatg
     2941 gtatcggtaa cccggccgag ttcgagaacg ggatcgtccg gctgcagcag gggcaggtga
     3001 tctcgcgcca ggggttcctg cattccctcg tcaattccct gtataagcga agtgagatcg
     3061 agtttgcgcg gggtaatttc cgtgtcaagg gggatacggt ggatatcaat ctgccgtatg
     3121 tagattatgg ctatcggatc agctttttcg gcgacgagat cgactcgatc gagagcttcg
     3181 atatccagac cggcaagcgc atcgccgccc tcgacaatgc cgccatcttt ccggctaatc
     3241 tctatcttgc gccaaaggat atcctgcacc aggtgatcta tgagatccag gacgagcttg
     3301 ccgaacaggt cacctacttc aagaacgtcg ggaagtatat cgaggcgcag cggcttagcg
     3361 aacgggtgaa ctacgatgtg gagatgatcc gggaattggg gtattgcaac ggcgtggaga
     3421 attattcccg ctttttcgac cggcggatgc ccgggacgag gcctttctgc ctgctggatt
     3481 attttccaaa ggatttcctt ctcgttctgg atgagagtca tcagaccgtg ccgcagatca
     3541 gcgggatgta tggcggtgac cgcagccgca aactggtgct ggtggactat ggcttccggc
     3601 ttcccagtgc gcttgataac cggccgctca acttccacga gttcgagtcc ctgaccgggc
     3661 agacgatcta tgtctccgcc acgccggggg actacgagct ggaaaagacc gagggggtcg
     3721 tcgtggagca ggtggtccgc cctacgggac tgcttgatcc gcctatcgag gtgcggccca
     3781 gcgccaacca gatagacgat ctgctcgatg agatagacaa gcgggtgaag aagggggacc
     3841 gggtgttggt gacgacgctg acgaagcgga tggcggagga gatggacaaa tacctgcacc
     3901 ggatcgacat caaatccaaa tatattcata gtgaggtcga tacgctcgaa agggtggaga
     3961 tcctgcggca gctgcgactt ggcgagatcg atgtgcttgt cggggtcaac ctgttgcggg
     4021 agggtctcga tctgccggag gtgtcgcttg tcgcgatcct cgatgccaat aaggaagggt
     4081 ttctgcgtaa tgaaaaatcc ctgacccaga cggcgggtcg cgccgcccgt aatgtggatg
     4141 ggatggtgat cttttatgcc gatacgatca cggtgagcat gcaaaggacg atggacgaga
     4201 cgctgcggcg aagggaaaag cagatcgcgc ataacgagaa gcatggcatc atcccgcgca
     4261 cggtcataaa gtccaaggag caggtctttg cccagacctc ggtgctggat atcaagggct
     4321 atgacccggc cgataagtat gcgcttgcac ccgatgagga actggtggcg gtggcagcgg
     4381 aagaacagga ggtttaccag accattccgc aggtggagaa ggccattgcg aagaccaaaa
     4441 aggaaatgga aaaggcggcg agagatctgg attttatgga agcggcaaga ttacgtgatg
     4501 agatgttcgg gttgcagaag aagctgaagg agatgaaggg gtaaattgcc taatttcgct
     4561 accatggaaa atacggataa gagattattt ctcctggacg ccctggcgct tgtttttcgc
     4621 gcctattatg cactgatcag caatccccgg ctcacaacca agggccggaa taccaatgcg
     4681 caattcgggt tcaccaatgc cctggtggat cttatcaaca atcagcagcc ttcgcatatg
     4741 gcggtgtgct tcgacacgga ggcgccgacc gagcggcata cggatttcgc ggactacaag
     4801 gccaaccggc aggaggctcc cgaggacctg ctgagtgcgc tgcccgatat caaggccatc
     4861 atcaagggtt tcaatatccc ctgcatagaa ttcgatggct atgaggccga tgacgtcatc
     4921 ggtacgttga gcaaacaggc cgcggcggcg gggtatgagg tgtatatggt cactcccgac
     4981 aaggactatg ggcaattggt atcggaaaag atccgcatct ataagcccgg ctaccagggc
     5041 ggcaccgcgg agatcctggg accgaaggaa gtttgtgaga aatggaatat caaggatgtg
     5101 gcgcaggtga tcgatatcct t
//
LOCUS       DATGFG010000670         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1376814, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000670 DATGFG010000000
VERSION     DATGFG010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376814"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..3128)
                     /locus_tag="VKQ52_12465"
     CDS             complement(141..3128)
                     /locus_tag="VKQ52_12465"
                     /inference="COORDINATES: protein motif:HMM:NF039643.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HLZ88056.1"
                     /translation="MQKPRILLILLIILSFACRAQEIPYKTCPNCWNPDSLGNHRAVI
                     RFNGPVVIAHTAGVTATQSPVAHAHISWRLLIADPFTHRIIVQDQKTGARIPNVSTAN
                     LTRESGDIFFEPASGNGIYYIYYLPYRNEGHSNYPKGAYWQPDTTIPVNTTAPINCTV
                     TTIESIDAFNTFYPMEVIATARETKALKAKYPARPFLLFPEDRDHPIRMKNDLPLRWI
                     KSGPGNTINITADKGEFYAYQIGLFALKALDSIQLRFNTPFASTCINTTGVSYDGRPF
                     TKQVNTATDQIQPLWCGLDIPQNARPGVYTGTVTIRAKNAAPQTLPLHITVTDHLQAD
                     GGAGRPAKMTRLKWLNSTLAQENTVIAPYTPLTIQGDTLIRLLGRSIRLNQQGFPEQI
                     QTFFTPEMTDIGPTPNNLLAENIHFHFTRRSDGKDIRLKTQGLQFTERSPGTLQWQAA
                     SIADELRMDVSASLEFDGFLAYTVRVTALQDLDLRDIVMHIPFQKEMATHLMGLGQKG
                     GYRPDSMYHWKWDVAHKNQDGAWIGAVNGGLQYSLRDEHYVRPLNTNFYLQKPLIAPA
                     SWANGDKGGIDIGIKGRSMLANNYSGARQLKKGDTLYYNFNLLITPFHTLDTDFQWAT
                     RFYHKYNNLDTVKATGATVVNIHHATPINPWINYPFIEAQKLKRYIDSAHQLGLKVKI
                     YNTVRELSDHAYETFALRSLGNEIYSSGPGKGFSWLQEHIRNDYIPAWFVPEIKDAAM
                     INSGMSRWHNYYVEGMNWLVQNVGIDGIYLDDVAFDRVTMKRIKRVLTKDGHPGIIDL
                     HSANQYNPRDGFNNSANLYMEHFPYLSRLWFGEYFDYEKNPPDFFLIEVSGIPFGLMG
                     EMLQGGGNPWRGMVYGMTNRMPWSDNADPRPIWKAWDDFGMQGTKMIGYWSPNCPVKT
                     GNPDIPATVYSKAGSAMVALASWAQQDTTANLDIDWQRLGIDKTHAAIEAPEIKNFQP
                     ARHFVPGEPIPVEKGKGWILIIR"
     gene            3208..>3911
                     /locus_tag="VKQ52_12470"
     CDS             3208..>3911
                     /locus_tag="VKQ52_12470"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ88057.1"
                     /translation="MKTRNRVIFFINKGVHLLDLAGAVQAFYEAGEYGCAYEMVFVAD
                     KTGPVCSAGLPFARLDHYSTVEAGAGDLLIVAGFELRELAETRKPALHGWLRKAGADG
                     AAICSVCTGAFLLAEAGLLDGRECTTHWKHTGRLQKDFPGVRVQTDRLFVKDRNIYTS
                     AGVTTGLDLALFLLEERHGPEFAYKVARELVMYIRRDGDASQESVYLQYRAHINNDIH
                     VVQDWIIHHLHKKIRI"
BASE COUNT          731 a          875 c         1357 g          948 t
ORIGIN      
        1 ggtatcggtt tgcgcaatat tcgtaaccgc gtgagctatt acaacggggg ggtcacaatt
       61 gactcccagc cggggaaagg atgcatcctt accataatca ttccccttgt tcctgccggc
      121 ggcatgaaat gaccgtttcc ttaccggatg atgagtatcc agccttttcc cttttccacc
      181 gggatcggct caccgggtac gaaatgccgg gccggctgga agttctttat ttcgggggct
      241 tcaatggcgg cgtgggtttt gtcgataccc agccgttgcc aatcgatatc caggttggcc
      301 gtcgtgtcct gttgcgccca ggatgccaat gctaccatcg cggagccggc tttgctgtat
      361 accgtagcgg ggatatcggg attgccggtc tttaccgggc agttggggct ccagtagccg
      421 atcatctttg tcccctgcat cccaaaatca tcccaggctt tccagatggg ccgtgggtcg
      481 gcgttgtccg accagggcat gcggttggtc atgccgtaga ccatcccccg ccaggggttg
      541 ccaccgccct gcagcatttc gcccatcagg ccaaagggga tgccgctcac ctcgatgaga
      601 aagaaatcgg gcgggttctt ttcataatcg aaatattccc caaaccagag ccggctgaga
      661 taggggaaat gctccatgta gagattggcg ctgttattga agccatcgcg ggggttgtat
      721 tgatttgcgg agtgcagatc gatgatgccc gggtggccgt cttttgtcag cacgcgtttt
      781 atgcgtttca tagtgacccg atcgaaggcg acatcatcga gatagatgcc gtcgatgccg
      841 acattctgca cgagccagtt catgccttcg acgtagtagt tgtgccatcg gctcatgccg
      901 ctattgatca tagcggcgtc ttttatttcc gggacgaacc aggcggggat gtagtcgttg
      961 cggatatgtt cctgcagcca ggaaaaacct ttgcccggtc cggaggaata gatctcattg
     1021 cccaggctgc ggagggcgaa ggtctcgtag gcgtgatcgg agagctcgcg cacggtgtta
     1081 tagatcttta ccttcaggcc gagttggtgg gcggagtcga tatagcgctt tagcttctgt
     1141 gcctcgataa agggataatt gatccagggg ttgatggggg tagcgtggtg gatgttaacc
     1201 acggtggcac cggtggcttt taccgtgtcg aggttattgt atttatgata gaagcgggta
     1261 gcccactgga agtcggtgtc gagggtgtgg aagggggtga tgaggaggtt gaaattatag
     1321 tacagcgtat cccctttctt tagttggcgg gcgccgctgt agttgttggc caacatggat
     1381 cgtcccttga tgccgatgtc gatgcccccc ttgtcgccgt tggcccagga ggccggggcg
     1441 atcaggggtt tttggaggta gaagttggtg ttcaggggac ggacatagtg ttcgtcgcgg
     1501 agggagtatt gcaggccgcc gttgacggcg ccgatccagg cgccgtcctg gttcttatgg
     1561 gcgacgtccc atttccaatg gtacatgctg tccgggcgat agccgccttt ttgtcccagg
     1621 cccatcaggt gggtggccat ttccttctga aaggggatgt gcatgacgat gtccctgaga
     1681 tcgaggtcct gcagggctgt tactcttacg gtgtaggcca ggaagccgtc aaattcgagg
     1741 gaggcggaga cgtccatccg gagttcgtcg gcgatgctgg cagcctgcca ttgcagggtg
     1801 ccgggtgatc tttcggtgaa ttggaggccc tgggttttca ggcggatgtc tttcccatcg
     1861 gatcgtcttg taaagtgaaa gtggatattc tcggcgagca ggttattggg ggtcggcccg
     1921 atgtcggtca tttcgggggt gaagaaggtc tggatctgtt cggggaagcc ttgctggttg
     1981 aggcggatgc tgcggcccag gaggcggatg agggtatcgc cctggatggt gaggggagta
     2041 tagggcgcga tgacggtgtt ctcctgtgcg agggtggagt tgagccattt gaggcgggtc
     2101 atttttgccg gcctgccggc gccgccgtcg gcctgcaggt gatcggtaac ggtgatgtga
     2161 agaggcaggg tttgcggcgc cgcgttcttt gcacgtatcg tgacggtgcc ggtgtagacg
     2221 ccgggtctgg cgttttgagg gatgtcgagg ccgcaccaga ggggttggat ctgatcagtt
     2281 gccgtattta cctgtttggt gaacggacgg ccgtcatagg acacaccggt ggtgttgatg
     2341 caggtggagg cgaagggggt gttgaaacgt agttgaatgc tgtcgagggc tttgagtgca
     2401 aagagaccta tttggtatgc gtagaattcg cctttatcgg cggtgatgtt tatcgtgtta
     2461 cccggaccgc tttttatcca gcgaagggga agatcgtttt tcatgcggat gggatggtcg
     2521 cggtcttcgg ggaagaggag aaagggtctt gcgggatatt ttgctttcag ggcttttgtt
     2581 tccctggccg tggcgatcac ctccatggga tagaaggtat tgaaggcgtc gatggactcg
     2641 atggtggtga cggtgcagtt gatgggtgcg gtagtgttta ccgggatggt ggtatcgggt
     2701 tgccagtagg cgccctttgg atagttgctg tggccttcgt tgcgataggg gagatagtag
     2761 atatagtaga tgccgttgcc ggaggccggc tcgaaaaaga tgtcgccgga ttcgcgggtg
     2821 aggttggcgg tgctgacgtt ggggatgcgg gcgcctgtct tctggtcctg gacgatgatg
     2881 cgatgggtga agggatcggc gatgagaagg cgccaggaga tatgggcgtg ggcgacggga
     2941 gactgggtag cagtgacgcc ggcggtgtgg gcaatgacca cggggccgtt gaaccggatg
     3001 acggcgcggt ggttgccgag cgagtcggga ttccagcagt tggggcaggt tttgtaggga
     3061 atttcctggg cccggcaggc aaacgaaagg atgatcaaca ggatgagcag gatgcgcggt
     3121 ttttgcatgc gtcgaaggta agggatgaaa acgatttgtc gattttcgtc caaattttga
     3181 ggaaattggg agatattagg acttattatg aaaacaagaa accgggttat tttctttata
     3241 aacaaggggg ttcatctgtt ggacctcgcg ggggcggtgc aggcctttta tgaggccggg
     3301 gagtacgggt gcgcctatga gatggtattt gtcgcggaca agacagggcc ggtgtgttcg
     3361 gcggggctcc cctttgcccg gctggaccac tattcgacgg tggaggccgg ggcgggcgat
     3421 ctgctgatcg tagccgggtt tgagctgcgg gaactggcag agacgcgaaa gccggcgctg
     3481 catggctggc tgcgcaaggc cggcgctgat ggggcggcga tctgttcggt ctgtacggga
     3541 gcctttctcc tggcggaggc cgggctgctg gacggtcggg aatgtacgac gcactggaag
     3601 cataccgggc gattgcaaaa ggatttcccc ggtgtgcggg ttcagaccga cagattgttt
     3661 gtaaaagaca ggaacatcta taccagcgcg ggagtaacga cggggctcga cctggcgctt
     3721 ttcctgctgg aagagcggca cgggccggag ttcgcctata aggtggcgag agagcttgtc
     3781 atgtatatcc ggcgggatgg cgacgcgtcg caggagagtg tctatctgca ataccgcgct
     3841 catatcaaca acgatatcca tgtcgtacag gattggatca tccatcacct tcataagaag
     3901 atcaggatcg a
//
LOCUS       DATGFG010000671         4960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_596719, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000671 DATGFG010000000
VERSION     DATGFG010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596719"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VKQ52_12475"
     CDS             complement(<1..180)
                     /locus_tag="VKQ52_12475"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88058.1"
                     /translation="MPDQLLTQLQRGDYRALARAISYVENGVKEYSALLAEWSPATTP
                     VIGITGPPGAGKSTLV"
     gene            231..947
                     /locus_tag="VKQ52_12480"
     CDS             231..947
                     /locus_tag="VKQ52_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLZ88059.1"
                     /translation="MRPGTEKKLYTKVRSKQLSLQHVCEVGVWLPEMSNILDFIVKDR
                     LRTTLVEPDPKSIAAIRTYFRDYPNVELLPYAAFDHHGTLELVQRNASTFVSTLPYSP
                     AQVNDNYHIQQEDRFAVECRRFDELDDASISLLSVDTEGSEWYVIKYLKSRPLVISVE
                     MHGKSYLNPFYGEIAGWMANEGYDKWYMDKTDIVYYKKGAFPVTGVEKLQLSLMAAFV
                     RLRRVRKRIGKFFTGRKAGK"
     gene            944..1948
                     /locus_tag="VKQ52_12485"
     CDS             944..1948
                     /locus_tag="VKQ52_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLZ88060.1"
                     /translation="MRSFIISRTDAIGDVVLTLPVAGVLRELYPQARIFFLGRSYTEE
                     VVKASVHIDAFLNWDEWKRMPAAEAAKAMKAVGADTIIHVFPDKRIALLARRAGIRER
                     IGTANRIYHWFTCNRLVKLSRRHSPLHEAQLNLQLLKPLGARDLYSLDEIGGYYGLTR
                     LQPLPPEIAGLPDPDRYNLVLHPKSRGSAREWGLDNFRELIGLLPRDQFRIFITGTAS
                     EGKLLQSLLREFPFLTDLTGRLSLGELMAFLSRVDGLVAASTGPLHLAAAVGIDALGI
                     YPPIRPMHPGRWAPIGPGAKVFVKEGDCEDCRKTGNCACMRDIRPALLRDYLLSLISK
                     "
     gene            1963..2607
                     /locus_tag="VKQ52_12490"
     CDS             1963..2607
                     /locus_tag="VKQ52_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HLZ88061.1"
                     /translation="MTNFIPVFPLGIVVYPGETLNLHIFEPRYKQLINECYTEAKPFG
                     IPTVIDNRLNEMGTLVKITELVKVHDNGEMDIRTQGMRVFRVLELIKTVPDKLFSGAI
                     VNYPDNIEGPGKRELMQKVVNAIRELHRMLNIEKDFHKPDEELASYDIAHHIGLSLEQ
                     EYELLGLLREEQRQEYIKRHLGKVLPVIAEMETLKERVKLNGHFKNLSSFKFED"
     gene            2610..3335
                     /locus_tag="VKQ52_12495"
     CDS             2610..3335
                     /locus_tag="VKQ52_12495"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLZ88062.1"
                     /translation="MATTLCFDFGNTRMKCAVFTDGNFAEERVLADDSDVTIRMLLDQ
                     YRPDRSILSSVIDHNPGLEVLLQQRTHFHKLGHQSRLPVTTPVGKPETIGSDRLALVV
                     AAVTLFPGKNNLVIGLGSAITYNYVNKYREFIGGGISPGVEMRFRSLQAFTAKLPLVK
                     ADWNFPLAGYDTRTNILSGVILGMAKEVDGMIGAYEEKYDNFNVLLSGGDAGYFTRHL
                     KKKIFADPYLIYKGLYAISEFNR"
     gene            3319..3909
                     /gene="lptC"
                     /locus_tag="VKQ52_12500"
     CDS             3319..3909
                     /gene="lptC"
                     /locus_tag="VKQ52_12500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HLZ88063.1"
                     /translation="MSSIDKVFVVLLVTVLTGVACNDKATVRPTTGPHKIIPEEATDL
                     VSLMSEPMTDSTGKLTARVKGKLTAPYMLRYQLADSPYAEFPRTLHVDFYNDSAKTES
                     QLDAHYGKYLQNQEKVYLRDSVVVKNLQKGDTLRCQELWWDSHTEKFYTDKPVRIYTP
                     DKILYGTGMEADQNFRWYTITKLTGTVLTAGNSIPK"
     gene            4042..4677
                     /locus_tag="VKQ52_12505"
     CDS             4042..4677
                     /locus_tag="VKQ52_12505"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HLZ88064.1"
                     /translation="MSDKRDHILIVAEELFGEKGFDGTSVRDIAQKAGVNLAMISYYF
                     GSKEKLLEALIVFRAEYAYGILEELNKDQSLSPWDKIDRLVEFYVDRILNNLRFHNIM
                     YQEATAVRSEEIKNRIIAIKMRNLEQITKIITDGQQKKLFRQVDIPMTVGTIMGTISS
                     YTQSRSYSCNILAIREDADDDAYRAKLGPRLKIHLKQLLRAHLDIRNETPA"
BASE COUNT         1204 a         1243 c         1402 g         1111 t
ORIGIN      
        1 gaccagcgtg ctcttgccgg cgccgggcgg tccggtgatg ccgatcaccg gagtagtggc
       61 gggcgaccac tccgcgagca gggcgctata ctcttttaca ccattctcca cataggaaat
      121 agcgcgcgcg agggcgcggt agtcaccccg ttgcaactgt gtcaatagct ggtccggcat
      181 agctgtacaa atataattat gtaatgatga aaacaattaa ttttaccggc atgagacccg
      241 gaacggaaaa aaagctctat acaaaagtcc gatcgaagca gctctcgtta cagcatgttt
      301 gcgaagttgg ggtctggctt ccggagatgt ccaatatcct cgattttatc gtaaaggatc
      361 gactgaggac gaccctggtg gaacccgatc ccaagagcat tgccgccatc cggacttact
      421 tccgggacta cccgaatgtc gagctgctgc cctatgccgc tttcgaccat cacggaaccc
      481 tggagctcgt ccagcgcaat gcctcgacct ttgtttccac cctgccctat agtccggcac
      541 aggtgaatga caactatcat atccagcagg aggaccggtt cgcggtcgaa tgccggcgtt
      601 tcgacgagct ggacgatgcg tctatctccc tgctgagtgt cgacaccgaa ggcagtgaat
      661 ggtatgtcat aaaatacctg aaaagccggc ccctcgtgat ctcggtggag atgcacggca
      721 agagctatct gaatccattc tacggcgaaa tagcaggttg gatggccaat gaagggtatg
      781 ataaatggta tatggacaag accgatatcg tttactataa gaaaggcgcc tttcccgtca
      841 cgggtgtcga gaaactgcaa ttgtcgctga tggccgcctt tgttcggctg cgccgcgtcc
      901 gcaagcggat cgggaaattt tttaccggaa ggaaagcagg aaaatgagga gttttattat
      961 cagccgcacc gatgccatcg gagatgtggt gctcactctg ccggtggccg gggtattgcg
     1021 ggagctctat ccgcaggccc ggatcttctt cctgggccgc agctatacgg aagaggtggt
     1081 gaaggcgtcg gtgcatatcg atgctttttt gaattgggat gaatggaagc gcatgcctgc
     1141 cgcggaggcg gcgaaggcca tgaaggctgt cggtgcggac acgatcatcc atgtattccc
     1201 cgacaagcgg atcgccctgc tggcgcgaag ggcgggtatc cgggagcgta tcgggacggc
     1261 caaccggatc tatcactggt ttacctgtaa ccggctggtg aaactatccc gccggcattc
     1321 tcccctgcat gaggcgcagc ttaatttgca attgctgaaa ccgctgggcg ccagggacct
     1381 ttattcgttg gacgagatcg gaggctacta tgggctgacc cggcttcagc cgcttccgcc
     1441 ggagatagcg gggctccccg acccggatag gtacaacctt gtattgcatc ccaaatcgcg
     1501 gggcagtgcc cgcgagtggg gattggataa tttccgggag ctgatcgggc ttttgccgcg
     1561 cgatcagttc aggatcttta tcaccggcac ggccagtgaa ggcaagctcc tgcaatccct
     1621 gctacgggaa ttcccgtttc tcaccgatct caccgggcgg ctttcgctgg gggaactgat
     1681 ggcctttctt tcccgcgtgg atggccttgt ggcggccagc accggtccgc ttcatcttgc
     1741 cgcggcggtc gggatcgacg ccctgggaat ttatccaccg atacggccga tgcacccggg
     1801 gcgctgggcg ccgatcggac ccggggcaaa agtctttgta aaagaaggag attgtgaaga
     1861 ttgccgtaag accggaaact gtgcctgcat gcgggatatc cggcctgcgc tactgaggga
     1921 ttacctgctc tcgctaatct caaaataggt atttttgctg ctatgacaaa tttcattcct
     1981 gtgtttcccc tgggtatcgt tgtctatccg ggagagacgc tgaacctgca tatattcgaa
     2041 ccgcggtata agcaactcat caatgaatgc tatacagagg caaagccctt tggcattcca
     2101 acggtgattg ataatcgcct gaatgaaatg gggacgctgg taaagatcac cgaactggtg
     2161 aaggtgcatg acaacggtga gatggatatc cggacccagg gcatgcgggt attccgggtg
     2221 ctggagctga taaagaccgt gcccgataaa ttgttcagtg gtgcgatcgt caattatccc
     2281 gacaatatcg aggggcccgg caagagggag cttatgcaaa aggtggtcaa cgccatccgg
     2341 gaactccacc ggatgctgaa tattgaaaaa gactttcata agccagatga ggaactggca
     2401 agttatgata tcgctcacca tatcgggctt tcccttgaac aggaatatga gctgcttggc
     2461 ctgctgcggg aagaacagcg gcaggaatat atcaaacgcc atcttggaaa ggtgttgccg
     2521 gtcattgccg aaatggagac gctgaaggaa agggtaaaat tgaacggaca cttcaaaaac
     2581 ctatcttcgt tcaaatttga agactagcca tggccacgac cctctgcttc gattttggca
     2641 atacccggat gaaatgcgcc gtctttaccg acggcaattt tgcagaagaa agggtattgg
     2701 ccgatgacag tgacgttacg ataaggatgc tgctcgatca atatcgcccc gacaggagta
     2761 ttttatcttc cgttatcgat cataaccccg gcctggaggt cctgctgcaa cagcgtacgc
     2821 attttcataa gctgggtcat cagtcccggc tgccggtgac gacgccggtg ggtaagcccg
     2881 agactatcgg gtccgatcgc ctggcgcttg tcgtggccgc ggtgaccctt tttcccggga
     2941 agaacaatct tgtcatcggg ctcgggtcgg cgatcaccta taactatgtg aataaatacc
     3001 gggaattcat cggcggcggc atttcgccgg gggtggagat gcgtttcagg tcgctacagg
     3061 cctttacggc caagctcccc ctggtaaaag cggactggaa cttcccgctg gcggggtatg
     3121 atacgcggac caatattctc agcggggtga tcctggggat ggcgaaggag gtggatggca
     3181 tgatcggggc ctatgaggag aaatatgaca actttaacgt gttattaagc ggcggggatg
     3241 ccggctattt cacccgtcac ctgaaaaaga agatatttgc agacccatat ttgatctata
     3301 aaggtttgta tgccatcagt gagttcaatc gataaggttt ttgtggtttt gctggtcacc
     3361 gtgctgacgg gggtagcctg caatgacaag gcgacggtgc ggccgacgac gggtccgcat
     3421 aagatcatac ccgaagaggc caccgacctg gtgagcctga tgagtgaacc catgacggat
     3481 tccaccggaa aactgacggc cagggtgaag gggaaactaa cggcgcccta tatgctgcgc
     3541 taccagttgg cggattcccc ttatgcggag tttccccgga ccctgcatgt ggatttttat
     3601 aatgattctg cgaagaccga gagccagctc gatgcccatt acggaaaata tttacagaac
     3661 caggagaagg tttacctccg cgattcggtc gtggtcaaga acctgcagaa gggagatacg
     3721 ctgcgctgcc aggagctctg gtgggacagc cataccgaga agttctatac ggacaagccg
     3781 gtgcgcatct acaccccgga taagatactc tatgggacgg ggatggaagc ggaccagaac
     3841 ttccggtggt acaccatcac caagcttacc ggcaccgtgc tgactgccgg caacagcatc
     3901 cccaaataag acatcccgat aattatcaat ttgttatcca gatcaccccg ccagcggcgg
     3961 ggatttttat taaaaattat caattaaacg tttgtttaat tcaaacgttt gtttaacttt
     4021 gtcctgcaat tagcacttta catgtcagat aaaagagatc atattctcat tgtagcagag
     4081 gagcttttcg gagaaaaggg gtttgacgga acgtcggtga gggatatcgc ccagaaggcc
     4141 ggggtcaacc tggccatgat ctcttattat tttggctcga aggagaagct gctggaggcc
     4201 ctgatcgtct tccgggccga gtacgcctac ggcattttgg aggagttgaa caaggatcag
     4261 tcgctgagtc cctgggacaa gatcgaccgg ctggtcgagt tctatgtcga ccggatcctg
     4321 aacaacctcc ggttccacaa tatcatgtat caggaagcca cggccgtccg gagtgaggag
     4381 atcaagaaca ggatcatcgc catcaagatg cggaacctcg agcagataac gaagatcatc
     4441 accgatggtc agcaaaagaa gctgttccga caggtggaca tcccgatgac ggtcgggacg
     4501 atcatgggaa cgatctcttc ctatacccaa tcccgaagct atagttgtaa tattcttgcc
     4561 ataagagaag acgcagacga tgatgcctat cgcgcgaagc ttggcccccg gctcaagatc
     4621 catctcaaac aattgctgcg ggcccacctg gatatccgga acgagactcc ggcgtaaagc
     4681 tgagcgttcg tgtggtggtg atgagaaaat aattgaacga agttaaatac aagcacaaca
     4741 tggaaaaaaa gttagcagga gttctatgct tcttactttt tacaatcttg gtagccgatg
     4801 cacagccgcg gacccttacc ctggatgagg cggtgcagtt gggactacag aacagcaaac
     4861 aattaaaaag atcgcaggag aagatcgacg aggcccttac ccagctggat caggcgaagg
     4921 atctccgtct tcccagcgcc aaggtgagct ttcaatatct
//
LOCUS       DATGFG010000672         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_453952, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000672 DATGFG010000000
VERSION     DATGFG010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453952"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VKQ52_12510"
     CDS             <1..1341
                     /locus_tag="VKQ52_12510"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HLZ88065.1"
                     /translation="FRHGLRTDAATHYEKGMDISGTVNALKRAALLIKELAGGEIASE
                     IVDVYPDPANKIEVTLKNHYLKKLSGKNYHPDAVKNILLSLGFEIIREGIDAIHVAVP
                     WHKPDISLPADLVEEIMRIDGLDNIAIPAAITISPSVEVGVSQASWKSAVAHYLVGQG
                     FNEILTNSITNSAYFSEQELETTVKMINSLTAELNVLRPSLLETGLESVGYNLNRKSG
                     QLRFFEFGKTYSTTGVGNYEETNHLCLYLTGQLQEDSWKGKGKEVDFYYVKGLCERIF
                     PILGLKKPDWEPLDHPKLETGLAVTVDGQALVGIGPVRPELLKRFDCKQEVFFVDFHW
                     DNLLHLALNKKIEFRELPRQLPVYRDLAMIVDQALPFERVEKAIRGAKLEKLQEVKLF
                     DIFESEKLGKGKKSLAVSFTFLDEEKTLTDKEIDTMVGRIMGALEKEVQAEIRK"
     gene            1392..1721
                     /locus_tag="VKQ52_12515"
     CDS             1392..1721
                     /locus_tag="VKQ52_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88066.1"
                     /translation="MSEETNSAGKQGPPAGEAELRVRRIQEKLQQLLRQRDLLLKENG
                     KLREELRQLQQGHSDQGLRLEQLQQQVEILKATKAAMSEGEKRALEKRLGQYIREIDR
                     CITMLGE"
     gene            complement(1726..1980)
                     /locus_tag="VKQ52_12520"
     CDS             complement(1726..1980)
                     /locus_tag="VKQ52_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88067.1"
                     /translation="MFLKVKSPVFIDHLTIQVDHPQKCLLRAVLKDDTGLVCRDLETE
                     VENDQQELDWNGLNDLPYGVYTLEISRGKDEMKMRLVKRV"
BASE COUNT          597 a          566 c          639 g          475 t
ORIGIN      
        1 ttccggcatg gcctgcgcac cgacgccgcc acgcactatg aaaaaggaat ggatatttcc
       61 ggcacggtaa atgcgctaaa aagggcggcc ctgctgatca aagaactcgc cggcggcgag
      121 atcgcttccg agatcgtcga tgtctatccg gaccccgcga acaagatcga ggtgaccctg
      181 aaaaatcact acctgaaaaa actcagcggt aagaactatc accccgatgc ggtaaagaat
      241 atcctgctgt cgcttgggtt cgagatcata agggaaggga tcgatgccat tcacgtggcc
      301 gtaccctggc acaaaccgga tatcagcctg ccggccgacc tggtggaaga gatcatgcgc
      361 atcgacggcc tggacaatat cgccatcccg gccgcgatca ccatctcgcc ctccgtcgag
      421 gtcggcgttt cccaggcttc ctggaagtcg gccgtggcgc actacctggt cggacaggga
      481 ttcaacgaga tcctcacgaa ctcgatcacg aacagcgctt atttcagcga acaggaactg
      541 gagacaacgg taaaaatgat caacagtctt accgcggagc tcaatgtcct gcgtccctcg
      601 ctgctggaga ccgggctcga gtccgtcggt tacaacctca accggaagag cggtcagctg
      661 cgctttttcg agtttggcaa gacctatagc accaccgggg tagggaatta cgaggagacg
      721 aaccatctct gcctgtatct caccggacag ctgcaggaag attcgtggaa gggtaaggga
      781 aaggaagtcg acttttatta tgtaaaagga ctttgcgaac gcattttccc gatactggga
      841 ctgaagaagc cggactggga acccctggat catcccaagc tggaaacggg tctggcggtc
      901 accgttgatg gccaagccct ggtcgggatc ggcccggtgc ggcccgaact gctgaagcgc
      961 ttcgactgca aacaggaagt cttttttgtc gatttccact gggacaacct gctgcacctg
     1021 gccctgaaca aaaagatcga gttccgggag ctgccgcggc agctgcccgt ctatcgcgat
     1081 ctggcgatga tcgtggacca ggccctgcct ttcgagcggg tggaaaaagc catccggggc
     1141 gcgaagctgg agaaattgca ggaagtaaag cttttcgata tatttgaaag tgagaagctg
     1201 gggaagggca agaaatcgct ggccgtcagc tttaccttcc ttgatgaaga aaagaccctg
     1261 accgataagg aaatcgacac aatggtcggc cgcatcatgg gagcgctgga gaaagaggtg
     1321 caggccgaga tacgcaagta acaggatcgg gagatgcccg atgatccacc ggtccgttta
     1381 aatggtcgcc aatgagtgaa gaaaccaata gtgcgggcaa gcagggtcct cctgccggag
     1441 aagcagagct aagggtcagg cggatccagg agaagctgca gcaattgttg cggcagaggg
     1501 atcttctgct gaaagagaac gggaagctta gggaggagtt acgtcagctg caacaggggc
     1561 attcggacca ggggctcaga ctcgaacaat tacaacaaca ggttgagata ctaaaagcga
     1621 caaaagcggc tatgagtgaa ggggaaaaaa gggcattgga gaaacgtctt ggccagtaca
     1681 tccgggaaat cgaccggtgt attaccatgc tgggtgaatg accggtcaga ccctttttac
     1741 caatcgcatt ttcatctcat cctttccacg cgatatttcc agggtataca ccccatacgg
     1801 caggtcgttg agtccattcc agtccaactc ctgctggtcg ttctctacct ctgtttccag
     1861 atcccggcag accaggccgg tgtcatcttt caggacggcc ctcaataaac atttctgtgg
     1921 atgatcgacc tggatggtta agtggtcgat aaaaacgggg gacttgactt ttaagaacat
     1981 ggctcttgta gttttaagat agggtattgg tgtcaacgtt cattttttat aaggatggtc
     2041 gatagtcaga gtcgcaaaac cggttaacct tccgccacgt cataatccct tgtgtataag
     2101 gtgtggataa atataaaaca agtatttcaa ctatgcaaga gttaatccca ataaatgttg
     2161 tcatcggtga ccggacctac cggatcaaga tcgagcccaa ggatgaagag gctgtccgga
     2221 agacgttgaa gctgatcaat gacaagctca tcgaatataa gacccagttt gcgggtc
//
LOCUS       DATGFG010000673         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1487905, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000673 DATGFG010000000
VERSION     DATGFG010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487905"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1001
                     /locus_tag="VKQ52_12525"
     CDS             180..1001
                     /locus_tag="VKQ52_12525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ88068.1"
                     /translation="MGRGNFFAIPVTDKVKISAVIITYNESRNIRRTLSRLYWCDEIV
                     VVDSYSTDDTVDICREFGCRVHFRHFDGYGAQKKHAVALAKNDWVLCLDADEVLSEEL
                     IGEIARVFAQGPVFSGYRLPMNLVFLGREFRHGRESQRYFLRLFRKTRGQFNEASVHE
                     RIELPDPAGKLKHALQHFSYDDLGHWYAKMDRYSTLGAEDAVRKGKRKSVFALVFALP
                     YYFLRYYFLERNFLNGVEGFYWAVLNSYYHFTKYAKIREIHSRPARLTLSQRFGG"
     gene            934..1704
                     /locus_tag="VKQ52_12530"
     CDS             934..1704
                     /locus_tag="VKQ52_12530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLZ88069.1"
                     /translation="MPRSGKSIAGLRVLLYHSVSGDDHRDGLTVTRQQLEQQFSYLRR
                     KGYTAILLSDLIAYCEGGKPLPRNPVLVTFDDGFLNNIKIAYPLAKDYGIKINLFVVP
                     VFMKRGGYRNEPCLQPEDIERLDPALVEIGLHSYSHPNYTYLLPAEIEADLDRCLYSL
                     QQMGIPYQPCLAYPFGAYPRRNDEAQSGFFELLEQKGIRLAFRIGNRVNALPLRQKFL
                     IQRLDIRGDEPFFFFRWSLAFGKKFFRLPGFLRRVGMA"
     gene            1763..2209
                     /locus_tag="VKQ52_12535"
     CDS             1763..2209
                     /locus_tag="VKQ52_12535"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HLZ88070.1"
                     /translation="MKTILDQSTRAELIARINALPTDSQAQWGRMTLYQMLKHCTLWD
                     EWMQGRRQYPRRSLLGRIIGRMALRSSVKDERPMMRNAMSLPELQITEDGDVVLQRAR
                     WIAAIEGYGHFSNPGFVHFFFGKMTEEEMGIFAYKHADHHLRQFNR"
BASE COUNT          453 a          564 c          721 g          568 t
ORIGIN      
        1 atgggtgttt tattatgatg atctgcgctg aggcgtgccg gaggtgtggg aaccgccggg
       61 gtgtgttgaa cggcggcgct aatttttttt gagccgggcg taggggtgcg gagtgtttcc
      121 tgcgttttat ccatatgcta accatacggg agacacccag agttcccccg gaaccttttt
      181 tgggaagggg gaacttcttt gccattccgg taacggacaa ggtgaagatc agcgccgtca
      241 tcattaccta taacgagagc cggaatatcc ggcggaccct gtcgcggctt tactggtgcg
      301 atgagatcgt cgtggtggac agctacagca cggatgatac ggtggacatc tgccgggagt
      361 ttggctgtcg cgttcatttc cggcattttg acggctatgg cgcgcaaaaa aaacatgcgg
      421 ttgcgctggc gaagaacgat tgggtgctgt gtctcgacgc cgacgaggtg ctttccgaag
      481 agctgatcgg ggaaattgcg cgggtgtttg cacaggggcc cgtcttttcc ggctaccggc
      541 tgccgatgaa ccttgttttc ctgggcaggg aatttcgtca tggccgggag agccagcggt
      601 atttcctccg gcttttccgc aagaccaggg ggcaattcaa tgaggcaagc gtccacgagc
      661 gtatcgaact gccggacccg gccgggaagc tgaaacatgc cctgcaacac tttagctatg
      721 acgacctggg ccattggtat gcgaagatgg accggtattc cacgcttggg gccgaggacg
      781 cggtaagaaa gggtaagcgc aaatctgtat ttgcgctcgt ttttgcactg ccgtattatt
      841 tcttacgtta ttactttctg gaacggaatt ttctcaacgg tgtcgagggc ttctactggg
      901 ccgttttgaa ttcctattat cattttacca aatatgccaa gatcagggaa atccatagcc
      961 ggcctgcgcg tcttactcta tcacagcgtt tcgggggatg atcaccggga tgggctgacc
     1021 gtcacccgcc agcagttgga gcagcagttc agctatctcc ggcgcaaggg gtatacggcg
     1081 atcctgctga gcgatcttat cgcctactgt gaagggggga agccgttgcc gcggaacccg
     1141 gtgctggtca cttttgacga tgggttcctg aacaatataa agatcgcgta cccgctggcg
     1201 aaggattacg ggatcaagat caatcttttt gtcgtcccgg tctttatgaa gcggggcgga
     1261 taccggaatg aaccctgtct tcagcccgag gatatcgaaa ggctcgatcc ggcgctggtg
     1321 gagatcggtt tgcattctta ttcccatccc aattatacct atcttttgcc ggcggagatc
     1381 gaggcggatc tcgaccgttg cctgtacagt ctgcagcaga tgggtatccc ctatcagccc
     1441 tgcctggcct atccctttgg cgcctatccc cggcgcaatg acgaggcaca aagcgggttc
     1501 ttcgagctgt tggagcagaa gggtatccgg cttgctttcc ggatcggcaa ccgggtcaat
     1561 gcgttgccgt tgcggcagaa attcctgatc cagcggctgg atatccgggg ggacgagccg
     1621 ttcttctttt tccggtggag tctggctttt gggaagaagt tcttccggtt gccggggttt
     1681 ctccggcggg tggggatggc gtaagttgga atttttcctt ccttttttgt ccggttgggg
     1741 acggtcgggt tgtctactgg gtatgaaaac catactcgac caatcgacaa gggctgaatt
     1801 gatcgcccgg atcaatgccc tgcctacgga cagtcaggcc caatggggca ggatgaccct
     1861 ttaccagatg cttaaacatt gcaccctctg ggatgagtgg atgcagggca ggcggcaata
     1921 cccgcggcgg tcgctgctgg gccggatcat cggcaggatg gcgttgcgca gttcggtgaa
     1981 ggatgaacgg ccgatgatgc ggaatgcgat gtccctgccg gagctacaga tcacggaaga
     2041 cggggatgtc gtcctgcaac gggccaggtg gatcgcggcg atcgaggggt atgggcattt
     2101 ttccaacccg ggttttgtgc attttttctt tgggaagatg acggaggagg agatggggat
     2161 ctttgcgtat aagcatgcgg atcatcattt gcggcagttc aataggtaag aaaagggccc
     2221 ccggtgaagt taccggaggc cctggataca gccgcttaca gcactttttc gagtttttcc
     2281 ccgagtgccg ggccccggag gttctt
//
LOCUS       DATGFG010000674         5498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1187467, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000674 DATGFG010000000
VERSION     DATGFG010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187467"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VKQ52_12540"
     CDS             complement(<1..382)
                     /locus_tag="VKQ52_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HLZ88071.1"
                     /translation="MSSPAKNFATWGLLFIAGIVFFSVGVLAFINPMSSYVKLTKFAG
                     VGLLVNGGLLLAMIIVRPMRPRERNWLQAESILHLFFGILFLLNPLLAFIALPYFIGS
                     WLFLVGALKILAALSLRHRLGRWGF"
     gene            734..913
                     /locus_tag="VKQ52_12545"
     CDS             734..913
                     /locus_tag="VKQ52_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88072.1"
                     /translation="MKALLSILVLVAIVFAYIISASLYVQGDLVLAYSLILASIASFV
                     LWIRSNDILKMLAKA"
     gene            957..1184
                     /locus_tag="VKQ52_12550"
     CDS             957..1184
                     /locus_tag="VKQ52_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88073.1"
                     /translation="MKTFVAFLTLPIIFLICFLAAKFYISGQYVAAYTLVLGWFASFT
                     LWIRNIGLLFQKPVVVAPRAPRAYIAGAKNR"
     gene            1277..2278
                     /locus_tag="VKQ52_12555"
     CDS             1277..2278
                     /locus_tag="VKQ52_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLZ88074.1"
                     /translation="MKKAKHEDLISAIESEDGIDRAGFIKCMAWAGTGVLWMMSGGVL
                     KSFGMSQLIDRNTGHIKPGLALPKSDFSFVQISDSHIGFNKPANPDVIGTLQAAIDKI
                     NTLPSAPSFVLHTGDLSHLAQKDEFDTLDQALKSIKTDKIFYVPGEHDVLNDNGKSYL
                     ERYGKGTKGQGWYSFDTGGVHFIGLVNVVDTAEGGLGVLGADQLQWLADDLKGLSNST
                     PVVVFAHIPLWAVYPQWGWGTRDSEQALALLKRFGSVSVLNGHIHQTMLKVEGNITFH
                     TATSTAFPQPQPGTAPAPGPMKVPAEKLRGLLGLTSVNYVEHAHSLAITDTPLISQP"
     gene            2275..2802
                     /locus_tag="VKQ52_12560"
     CDS             2275..2802
                     /locus_tag="VKQ52_12560"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ88075.1"
                     /translation="MRTCFVLLLACLAAGPLSAQLQPVDNGSSVQFKIKNLGFNVTGT
                     LTGLSGQIRFDPAHPEEAAFDVSVDANTINTDNGMRDDHLRQETYFNVQHYPRIRLLS
                     GKISLHKRGAYLFAGRLTIKDHTKDVVFPFTVVEIDGGYRFQGVFTISRRDFEVGGFS
                     TISDELEVTLNIIAK"
     gene            2809..3258
                     /locus_tag="VKQ52_12565"
     CDS             2809..3258
                     /locus_tag="VKQ52_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLZ88076.1"
                     /translation="MLLNNRSLVTAALVILVAIGSIAATSTAPVTATSVTPATTPVTA
                     PNGEYTNLKVLPKDISSKTLQSIMIDDFEDGLGVGCGFCHAEQKGSHKLDYASDAKPE
                     KLIARNMIRMTLKLNAKYFQVHRPGLGSSGLVVTCTTCHQGKPRPGE"
     gene            3349..3633
                     /locus_tag="VKQ52_12570"
     CDS             3349..3633
                     /locus_tag="VKQ52_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88077.1"
                     /translation="MPPVRINNVTVTQMMGNHLVVLTAPTADYDVAPAAVGVIVNAAL
                     TQQNGGPVTGPFPTGNVYTVSGDAGGVIHQIPHVRYIGHTPGGNAQFTTQ"
     gene            3748..4617
                     /locus_tag="VKQ52_12575"
     CDS             3748..4617
                     /locus_tag="VKQ52_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ88078.1"
                     /translation="MKRTSGFIAIVSAVLLFSCVSTRKFNAMQQADRAKYDSLYASSQ
                     SQLQSCRDANADLSRQNTGLQNQLNDLNRQLPLIRENNTQLLKQLQDLSVISSSQAES
                     IRKSLENIGAKDTYIRDLQSALARRDSLNLALVMNLKGVIGNLDDKDINIKVEKGVIY
                     IDISDKLLFNSGSYTVTENAKVVLGKVALVLKNQPDIEFMVEGHTDSVRYAHGDLLDN
                     WDLSVKRATSVIRILQFQYNIPPERMTAAGRSEYITVASNSTVEGRAANRRTRIVILP
                     QLDQFFKLLEPKQ"
     gene            4726..>5498
                     /locus_tag="VKQ52_12580"
     CDS             4726..>5498
                     /locus_tag="VKQ52_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ88079.1"
                     /translation="MRNLPRNILLTGATGILGSHVLYELLIACIKAHTQTRITLVVRS
                     TDKETARERIIGVLSNPYRPYLLHAFTTEELLSFVNIIDAGLNELRDPHLAGLERRGP
                     LHIIHCAAHTNLSVNSNVYDTLYQHNYMGTLHLLQWASAILEKFTFIGGAYSIGHRGG
                     IIPDSYKQLTSTGEKAEIAGRPWRNPYERIKAEAGYAVIDFCTRYGIKWQILRPTTIC
                     GRIMYRPLFYTPKFNVFYLFGKFFQNAARSNNRNEVVRVL"
BASE COUNT         1373 a         1622 c         1322 g         1181 t
ORIGIN      
        1 tgaaccccca tctccccagg cggtggcgaa gagaaagcgc ggccagtatc ttcaatgccc
       61 ccacgaggaa aagccatgac ccgataaaat agggcagggc gataaaggca agtaaagggt
      121 tcaacaggaa aagaatgccg aagaacaaat gcaggatact ctcggcctgc agccagtttc
      181 gctcccgtgg gcgcatgggc cggacgatga tcatggccag cagcagccct ccgttcacca
      241 gcaacccgac accggcgaac ttcgtcagtt ttacataact gctcatcggg ttgataaagg
      301 cgagaacgcc gacgctgaag aagacgatgc ccgcgataaa gagcagcccc caggtggcga
      361 aatttttagc tggtgaagac atatagcaat atacacagaa tcaacgcaaa atgtaagccg
      421 cctcacgcaa aggggcagct tttttgttga taaatacccc tagaccaccc cagaattttc
      481 aaaaaaagct tgcatagctc aaaaacgcac actactttta caccatcaac ggcacacgcc
      541 tccaatctcc ccgataaacc agcgtcgtac ccaatctccc tcgaaacacc tccatttagc
      601 ttcctgtaat tcttactgat ccttcttcaa ttatcctctg caattacctt cgcacccaat
      661 gactatgagc tacccccaat agcgtaattc ttattttaac catagtcttt taatcaccta
      721 aaacgtaccc agtatgaaag ctcttcttag catcctcgtt ctcgtagcca tcgtttttgc
      781 ctacatcatc agcgcttctc tttatgttca gggcgacctc gttcttgctt actctctcat
      841 ccttgcttcg atcgcttctt ttgttctctg gatcagatcc aacgacatct tgaaaatgct
      901 ggcaaaagca taagcttacc aaaacttctt atctctaaca ccgcaaaatc acaaaaatga
      961 aaactttcgt tgccttcctg actcttccca ttatcttcct gatctgcttc ctcgcagcaa
     1021 aattctacat ctccggccaa tacgtcgccg cctataccct ggtgctcggc tggtttgcct
     1081 ccttcacgct ctggatccgg aacatcggct tactgttcca aaaaccggtt gtcgtcgcgc
     1141 cccgcgcacc ccgggcctat atcgccggtg cgaaaaaccg ctaaatcgcc aatttgatca
     1201 agaatccggc ccgcctcatg taaaagattt cagccgcgcg cagagaacag cgttttttgc
     1261 tttacaattt cagtatatga aaaaagcaaa acacgaggat cttatcagcg caatcgaaag
     1321 cgaagacggc atcgacaggg ccggttttat caaatgcatg gcctgggcag gcacaggcgt
     1381 actctggatg atgagcggcg gcgttctcaa atcctttggg atgagccagc tcatcgacag
     1441 gaacacgggc catatcaaac cagggctcgc actgcccaaa tccgacttca gtttcgtcca
     1501 gatcagcgat agtcatattg gcttcaataa acccgccaat cccgacgtca tcggcacgct
     1561 ccaggcggcg atcgataaga tcaataccct gccatcggcc ccatcctttg tcctgcatac
     1621 cggcgacctt tcccacctgg cgcagaagga cgagttcgat acactcgacc aggcgttgaa
     1681 aagcataaag accgacaaga tcttctatgt tcccggtgaa cacgatgtcc tgaacgacaa
     1741 cgggaagtcc tatctcgaac ggtatggcaa gggcacaaag ggacagggct ggtacagctt
     1801 cgacacgggc ggcgtacact ttatcggatt ggtcaatgtc gtggataccg ccgaaggcgg
     1861 cctgggcgta ctgggcgccg atcagctgca atggctggcg gatgacctca aagggctgtc
     1921 gaacagcaca ccggtggtcg tgtttgccca tatcccgctc tgggccgtct atccccaatg
     1981 gggctgggga accagggaca gcgaacaggc ccttgccctc ctcaaacgat ttggctctgt
     2041 gtcggtgctg aacggacata tccaccagac gatgctgaag gtagaaggca atatcacctt
     2101 ccacacggcg acatcgacgg cctttcccca gccgcaaccc gggacggcgc ccgcgcccgg
     2161 accgatgaaa gtgcccgccg agaaactgcg cggcctcctc ggcctcacca gcgtgaacta
     2221 tgtcgaacac gcccattccc ttgcgatcac cgacaccccg ttaatcagcc agccatgaga
     2281 acctgcttcg tactattgct ggcctgtctg gccgccgggc cgctttccgc gcaactccag
     2341 cccgtcgaca acggctcctc cgtacagttc aaaatcaaaa acctgggctt caatgtgacc
     2401 gggaccctca ccggcctttc cgggcagatc cggttcgatc ctgcccaccc ggaggaagcg
     2461 gccttcgatg tttccgtcga cgccaatacc atcaataccg acaacgggat gcgcgatgat
     2521 catcttcgac aggaaacgta ttttaacgtg cagcattacc cgcgtatccg gctgctttcg
     2581 gggaagatat cccttcacaa aagaggcgcc tacctgtttg ccggccggct caccatcaag
     2641 gatcacacaa aggatgtcgt ttttcctttt accgtagtgg agatcgatgg gggctaccgg
     2701 ttccagggag tttttaccat cagccgaagg gattttgagg tcgggggctt cagcaccatc
     2761 tccgacgaac tggaagtcac attaaatata atcgccaaat aaagaaagat gctgttaaac
     2821 aatcggtcgc tggtaacggc agccctcgtc attctcgtgg ccatcggttc catcgccgcc
     2881 acgtccacgg ccccggtaac cgccacttcc gtaactccgg caaccacccc ggtgaccgcc
     2941 cccaacggcg aatacaccaa cctcaaagtt ctcccgaagg atatctcttc caaaaccctg
     3001 caaagcatca tgatcgatga cttcgaggac ggcctcggcg tcggctgtgg tttctgtcac
     3061 gcggagcaaa agggctcgca taagctcgac tatgccagcg acgccaaacc ggaaaagctg
     3121 atcgcccgca acatgatacg gatgacgctg aaactgaacg caaaatattt ccaggtgcac
     3181 cggccggggt tggggagctc cggtctggtg gtcacctgta ctacctgtca ccaggggaag
     3241 ccccggccgg gggaatgacc aggtgattct acctgttttc cggaacaggc ctttccactc
     3301 ttttcttcgc ccgcgattgg tgctaacatt gcctaaaacc aatgtaacat gccaccagtc
     3361 agaatcaaca acgtcaccgt gacccagatg atgggcaacc acctcgtggt cctcacagcc
     3421 ccaaccgcgg attacgacgt agcgccggca gccgtgggcg ttatcgtcaa cgcggcgctt
     3481 acacagcaga acggcggtcc cgtcaccggc cctttcccta ccgggaatgt ctataccgtc
     3541 agcggggacg ccggcggagt aattcaccag atcccgcatg tccggtatat cgggcatacc
     3601 ccgggaggaa atgcgcaatt cacaacgcag taaggggagg gccctccccc tttgcgtgat
     3661 catcatcaag ggtttgtgtg ataagggtca tatatgccgc cgtttttcac ctttaccttt
     3721 acggcgcatg tataaaaccc tttttatatg aaacgcacat caggctttat cgccatcgtc
     3781 tcggccgtcc tccttttctc ctgcgtgagc accaggaaat tcaatgccat gcagcaggca
     3841 gaccgggcta aatacgactc cctttacgcc agctctcaaa gccaactgca atcctgcagg
     3901 gacgccaacg cggatctcag ccggcagaac accggtctgc agaaccagct caacgatctg
     3961 aaccggcagt tgccccttat cagggagaat aatacgcaac tgctgaaaca gttgcaggat
     4021 ttgtcggtta tcagcagctc ccaggccgaa agcatcagga aatccctgga aaatataggc
     4081 gcaaaggata cttacatccg ggacctgcaa tcggctcttg cccgccgcga ctcgctgaat
     4141 ctcgccctgg tgatgaacct gaaaggcgtc atcggcaatc tcgacgataa ggacataaat
     4201 attaaggtag agaaaggggt tatctatatc gatatctccg ataagctgtt attcaacagc
     4261 ggcagctata cggtaacgga gaacgcaaag gtcgtactcg gtaaggtggc gctggtactg
     4321 aaaaaccagc ccgatatcga attcatggtc gaagggcata cggacagcgt gcggtatgca
     4381 catggagatc tgcttgacaa ctgggatctc agcgtcaaac gcgcaacgtc cgtcatccgg
     4441 atactgcaat tccagtataa catcccaccc gaaagaatga ccgctgcggg gcgcagtgaa
     4501 tacattaccg ttgcttccaa ttccacggtg gaaggccgtg cggccaaccg gcgcacgagg
     4561 atcgtgatcc tgccacaatt ggaccagttc tttaaattac tagaacccaa acaataattg
     4621 acgcgggcat agaatagtta aaggtttatt tttgttcttt tccaacaatt ccgattgttc
     4681 cggcggagga tacccggtag tttagcctga ttaaaggaca tgactatgag gaacctcccc
     4741 cggaacatat tgctcaccgg cgccaccggt attcttggca gccacgtgtt gtacgaattg
     4801 ctgatcgcct gcatcaaagc ccatacccag acgaggatca cactggtggt gcgatcgacg
     4861 gacaaagaaa cggcgcgtga gcggatcatc ggcgtcctct cgaaccccta ccggccctat
     4921 ctccttcatg cctttacgac agaggaatta ttgtcgttcg tcaatatcat cgatgccggg
     4981 ctgaacgaac tgcgggatcc acacctcgcg ggtctggaga gaaggggccc ccttcacatc
     5041 atccattgcg ccgcgcacac caatttgtcg gtcaactcca atgtgtacga tacgctctac
     5101 cagcacaact atatgggcac gctccacctg ctgcaatggg catccgccat cctggaaaaa
     5161 ttcaccttca tcggcggcgc gtattccatc ggccaccggg gagggatcat cccggacagc
     5221 tataaacagt tgacctcaac gggtgaaaag gcggaaatag ccggccgtcc ctggagaaac
     5281 ccctacgaaa ggataaaggc ggaagcgggc tatgccgtca tcgatttttg tacccggtat
     5341 gggatcaaat ggcagatcct ccggcctact actatttgcg gcaggatcat gtatcgcccc
     5401 ctgttctata cgccgaaatt caacgtattc tacctgtttg gcaaattctt tcaaaatgcg
     5461 gcgagatcca ataacaggaa cgaggtggta cgcgtcct
//
LOCUS       DATGFG010000675         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_940785, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000675 DATGFG010000000
VERSION     DATGFG010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940785"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /gene="hutU"
                     /locus_tag="VKQ52_12585"
     CDS             <1..1471
                     /gene="hutU"
                     /locus_tag="VKQ52_12585"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HLZ88080.1"
                     /translation="IRALKDLGSDETLLIQSGKPVGILRTHPDAPRVLLSNSQLVPKW
                     ADWEHFDELEKKGLMMYGQMTAGSWIYIGSQGIVQGTYETYAAAAAKHFNGTLRGTLN
                     VTAGLGGMGGAQPLAITMNDGVALIAEVEEWRIDKRLETRYLDRKMDDIDAAIDLALE
                     AKAMKEPLSIGVRCNAVHLLERLLQRNCIPDTLTDQTSAHDPLIGYWPHELSYEQAVV
                     LRRENPEQYTDYAYRSMRRHVECMLELQARGAITFDYGNNLRARAKERGLANAFDFPG
                     FVPAYIRPLFCLGKGPFRWAALSGDPADIAVTDKVIAGLFPENDSLQRWLKLAQEKIA
                     FQGLPARICWLGQGEREKAGLAFNELVRTGAVKAPIVIGRDHLDTGSVASPNRETEAM
                     LDGSDAVADWPLLNALVNTAGGASWVSLHHGGGVGMGYSIHAGMVIVADGTPEAAARL
                     ARVLRNDPGMGVIRHADAGYELAKTTAREHGLDISQRVQ"
     gene            1690..>2055
                     /locus_tag="VKQ52_12590"
     CDS             1690..>2055
                     /locus_tag="VKQ52_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88081.1"
                     /translation="MNRRLATLAFVCVFSGLLVLFSCTKNANPTPPIHDTVTVIKNDT
                     TKLTDTLYASKPDTTVNLTKGLLVYLPFSGNIADSSGNGNPTQAVGSVLTYDAHGYAN
                     SAFGGTGNGEKVIVTNNGSI"
BASE COUNT          455 a          619 c          573 g          408 t
ORIGIN      
        1 catcagggca ttgaaagacc tcggatccga cgagaccctg ctgatccaga gtggtaaacc
       61 ggtgggtatt ctccgtaccc atcccgatgc accaagggta ttgctatcca attcgcagct
      121 ggtgcccaaa tgggccgact gggagcattt cgatgagctc gaaaagaaag ggttgatgat
      181 gtatgggcag atgacggccg gctcctggat ctatatcggc agccagggga tcgtacaggg
      241 aacctatgag acctatgccg cggccgccgc aaaacatttc aacggaacgc tgcgcggcac
      301 attgaatgtc actgcgggcc tgggcggaat gggcggcgcc cagccgctgg ccatcacgat
      361 gaatgatggc gtcgcgctta tcgccgaagt ggaggaatgg cgcatcgata aacggctgga
      421 gacccgctac ctggacagaa aaatggacga tatcgacgcc gcgatcgatc tcgccctgga
      481 ggcgaaagcc atgaaggagc ctctttccat cggggtccga tgcaatgccg tccacctgct
      541 ggaacggctg ctgcaacgca actgcatccc cgataccctg accgaccaga catcggcgca
      601 tgacccgttg atcggctact ggccgcacga actgtcctat gaacaggcgg tcgtcctgcg
      661 ccgggagaac ccggaacaat ataccgacta tgcctaccgg agcatgcgcc gtcatgtgga
      721 gtgcatgctc gagctgcaag cccggggcgc gatcactttc gactatggca ataacctgag
      781 ggcaagggca aaagagcggg gattggccaa tgcctttgat ttccctggat ttgtgcccgc
      841 ctatatccgc cctctcttct gcctggggaa gggccccttc cggtgggccg ccctcagtgg
      901 cgatcccgcc gatatcgcgg tcaccgacaa ggtgatcgcc gggctattcc ccgaaaacga
      961 ctcccttcaa cgctggctga aactcgcaca ggaaaagatc gcttttcagg ggcttccggc
     1021 ccgcatctgc tggctgggac agggagaaag ggaaaaggcc ggtctcgcgt tcaacgagct
     1081 ggtacgcaca ggcgcggtaa aagcgccgat cgtgatcggc cgtgatcacc tcgacaccgg
     1141 gtccgtcgcc tcccccaacc gggagaccga ggccatgctc gatggcagcg acgcggtcgc
     1201 cgactggccc ctgctgaacg ccctcgtcaa taccgccggc ggcgcatcct gggtaagcct
     1261 gcatcacggc ggcggagtgg gaatggggta tagtatccat gcggggatgg tcatcgtcgc
     1321 ggatggcacc cccgaggccg cggcccgtct ggcccgcgtt ctgcgcaacg accccggaat
     1381 gggggttatc cgccatgccg acgccggtta cgaactggcc aaaacgacgg ccagggagca
     1441 tggcctcgat atcagccaac gggtgcaata agtctgcagt cctgcccgac acgattaaga
     1501 gacgaattgt cacggctatc ggcattctga cacagttatg aacttttccg aaatatgtca
     1561 atatccggct atattttagc gtttactatt taatgttact cctaaccgac ctcccattaa
     1621 cctttacttt atccatgaaa ggaggggccc ctgtggctcc cttttttcaa taacctttaa
     1681 tcaatatcca tgaaccgtag acttgcaacc cttgcctttg tttgtgtttt ctcgggatta
     1741 cttgtgttat tttcctgtac caagaatgcc aatcccacac cgcccattca tgacaccgtc
     1801 accgtgatca agaacgacac gaccaaattg acggataccc tctatgcttc aaaacccgat
     1861 acgaccgtca acctcaccaa ggggctcctg gtctacctgc ccttctccgg aaatattgcc
     1921 gactccagcg gcaacggcaa tcctacccag gcggtgggca gcgtcctcac ctacgacgcg
     1981 catggctatg ccaacagcgc attcggcggc actggcaacg gggagaaggt gatcgttacc
     2041 aataacgggt ccatc
//
LOCUS       DATGFG010000676         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1507254, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000676 DATGFG010000000
VERSION     DATGFG010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507254"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VKQ52_12595"
     CDS             <1..1285
                     /locus_tag="VKQ52_12595"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88082.1"
                     /translation="LSPGLLAPSQVYTTDASATSAVLTLYSYYPTTNSLGYFTYAGGL
                     AADELQTSSSTADLLQFAASSVTATNTTMDNYLWIYPYQVIREANQAIAGLNASTSVS
                     AATKSQLLGEALFFRAFYFFHLVNYFGPVPLTLNTTEIDNAAIGRTDTATLYAQMITD
                     LKQAESLLPSAYVTTLHARVNKYAAAALLARIYLYTNDYPDAETEATKVIGATDVTYS
                     LPAPASTFVNTSPETILQFATLYGYSMFGAGYRTTSASTPPTYYLFNNFVGSFESGDL
                     RRSAWIDSAGSGTGLYYRINKYKLATATAGNEYNVVLRLAEQYLIRAEARAHLYTDLT
                     GAKADLNTVRARAGLGATTATTSQSLLTAILQERKVELFGESAHRWFDLKRTGQANTV
                     LGAAKPSTWQPTAVLLPIPQTELLLNNKLTQNPGY"
     gene            1304..>2020
                     /locus_tag="VKQ52_12600"
     CDS             1304..>2020
                     /locus_tag="VKQ52_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88083.1"
                     /translation="MIKRLLHCAAGILLATTIASAQQSPVADTGQRSLPRLYLLLQSS
                     HEADWLTAANSFYQLKRQLTVDSLQKAERIRFPHGVLVRMDSLQTVYHAKSAKEKEAA
                     FKRWIRQFPQEKTDPDVSYDYARHNVGREYALEDNVPKAMQYAGMFVSRPWKGEGAAG
                     IAYVLADKGHYKEAGILLQRAIDNAVAFMTFRRNENGAAFAATGYVSYCTKYAEILYH
                     EKQYDSALKYVAIAHDSAKKV"
BASE COUNT          473 a          709 c          470 g          368 t
ORIGIN      
        1 gctctccccc ggtctgctgg cgccttccca ggtatacacc accgatgcct ccgccaccag
       61 cgccgtattg accctgtatt cctattatcc gacaaccaac agcctgggct attttaccta
      121 tgccggcggc ctcgccgccg atgaattgca aacctcttcc tctaccgccg atctgctcca
      181 gtttgccgcc tcctccgtca cagccactaa caccaccatg gacaactacc tgtggatata
      241 tccctaccag gtgatccgcg aggcaaacca ggccatcgcc ggactgaatg ccagcaccag
      301 cgtcagcgcc gccaccaaaa gccagctcct cggcgaagcc ctcttcttcc gcgccttcta
      361 tttcttccac ctggtgaact atttcggtcc ggtacccctc accctcaaca cgacggagat
      421 cgataacgcc gccatcggcc ggacggatac ggctaccctc tacgcccaga tgatcaccga
      481 cctcaaacag gcagagagcc tgctcccttc cgcttacgta accaccctgc atgcccgtgt
      541 gaacaaatac gccgcggcgg ctctgctggc gcggatctat ctgtatacga acgattaccc
      601 cgatgcggag accgaagcga caaaggtcat cggcgccacc gacgtgacct actccctccc
      661 cgcgccggca tccaccttcg tcaataccag cccggagacc atccttcagt ttgcgaccct
      721 ctatggctat tcgatgttcg gtgcgggcta ccgcaccacc tcggcatcta ccccgcccac
      781 ctattacctt ttcaacaatt ttgtcggttc cttcgaatcc ggtgacctcc gccggtccgc
      841 atggatcgac tccgctggtt ccggcacagg actctattac aggatcaaca aatacaaatt
      901 ggcgacggcc accgccggca acgaatacaa tgtcgttctt cgcctcgcgg aacaatatct
      961 tatccgggcg gaggcaagag cacatctcta tacagacctc accggcgcaa aggccgatct
     1021 caacacggtc agggccaggg ccggcctcgg cgccaccacg gccacaacaa gccagtcgct
     1081 cctcaccgcg atcctgcagg aacgaaaggt ggaactgttc ggggaatctg cacaccggtg
     1141 gttcgacctg aaacgcaccg ggcaggccaa taccgtcctc ggcgcggcca agccctccac
     1201 ctggcaaccc acggcggtac tcctccccat accccagaca gaactcctcc tgaacaacaa
     1261 actcacacaa aatcccggat actaaagcca caaatcatct tccatgataa agagactcct
     1321 ccactgcgcg gcagggatcc tgctcgccac gaccattgct tcagcacaac agtcaccagt
     1381 agccgatacc gggcaacgat ccctgccccg cctctatctt ttgctgcaaa gcagccacga
     1441 agccgactgg ctcacggcgg ccaattcctt ttaccagctg aagcgacagc tgaccgtcga
     1501 ctccctgcag aaggctgaac gcatccgttt tccccatggt gtcctggttc gcatggactc
     1561 cctgcaaacg gtctatcacg ccaaaagcgc aaaggaaaag gaagccgcct tcaaacgatg
     1621 gatcaggcaa ttcccccagg aaaagacaga ccccgatgtc tcctacgact atgcccggca
     1681 caatgtcggg cgcgaatatg ccctggagga taatgtaccg aaagcaatgc agtacgccgg
     1741 gatgttcgtg agccgcccct ggaaagggga gggggccgcc ggcatcgcct atgtccttgc
     1801 cgacaaaggc cactataaag aagccggcat cctgctgcaa agagccatcg acaacgccgt
     1861 cgcgttcatg acctttcgca ggaacgaaaa cggcgccgcc tttgccgcca cagggtatgt
     1921 gagctattgc accaaatacg ccgagatcct ttaccacgag aaacagtacg acagcgccct
     1981 gaaatatgtt gccatcgccc acgacagcgc aaaaaaagtg
//
LOCUS       DATGFG010000677         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1030517, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000677 DATGFG010000000
VERSION     DATGFG010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030517"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VKQ52_12605"
     CDS             <1..210
                     /locus_tag="VKQ52_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88084.1"
                     /translation="GALPRLVRLIRFTDNGKATNPMAWFYNHCCKPLSLGHEENASVL
                     YALTLVTLYWLICKQLDKRKIYIKV"
     gene            275..1036
                     /locus_tag="VKQ52_12610"
     CDS             275..1036
                     /locus_tag="VKQ52_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLZ88085.1"
                     /translation="MKSSILSILSFLVLQTAFAQRATPDVVPASGGSITIQPIVHASL
                     VLEWKNLTIYIDPSGGAANYTGIKPPDLIFITDIHGDHFDLNTLSAVRTSSTAIIVPK
                     AVADKMPDSLKSRIVILNNGDKSSQSGITVTALPMYNLPESPTAMHTKGRGNGYVLGI
                     GGKNLYISGDTQGIPEMRALKNIDIAFVCMNLPYTMDVNEAADAVLAFKPKTVYPYHY
                     RGQGGLSDVNAFKAKVEAGNKDIKVVLKNWYPTKG"
     gene            complement(1369..1977)
                     /locus_tag="VKQ52_12615"
     CDS             complement(1369..1977)
                     /locus_tag="VKQ52_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88086.1"
                     /translation="MQNKLLIADDHSMTRKGLKLLVENHIGKERVQEVWTCNQLMNEL
                     QTKTYTHLILDIFLSDGSTLEVIPTIRGLYPELEILIFSMQPISVYERALFPYRVRYF
                     ISKAANEVETLRMIGQFLTNQRPMAPISISEEDALPFSGMPAREMDVLHYLLKGWKTT
                     QIADTLNLSVSTVSTLKSRIFERTRTKNVIELMELANIHNVN"
     gene            2275..>2739
                     /locus_tag="VKQ52_12620"
     CDS             2275..>2739
                     /locus_tag="VKQ52_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88087.1"
                     /translation="MFRLWRTLKYAGAGGFYESDIRRAVCIGVNTLCVSIFLLNLFAG
                     LIFFLLSGKLGILVGAYVEGFLMLGIVALNYRRRYVLANILFYFVINLATFYFGAILG
                     PYAQVQLMFLFILGLIFFVFDDIAGRIICILITLLLLTLLEIQYRHPFIQPAS"
BASE COUNT          683 a          817 c          565 g          674 t
ORIGIN      
        1 ggcgccctgc cccggctcgt caggcttatc cgtttcaccg acaacggcaa ggccaccaat
       61 cccatggcct ggttttacaa ccactgttgc aaacccctgt ccctcggcca tgaagagaac
      121 gcctccgtcc tctatgccct caccctcgtg accctctact ggctcatctg caaacaactc
      181 gacaagcgaa agatctatat caaggtctag gcgaaaacgc acaaactata atataccagg
      241 acttccttac ttttatgtcc tcaaaacaaa aagcatgaaa tcctccattc tatccatcct
      301 gtccttcctt gtcctgcaaa cagcgttcgc ccagcgcgct acccccgacg tggtccccgc
      361 ctcgggcgga tcgatcacta tccaacccat cgtccacgcc agcctcgttc tcgaatggaa
      421 aaacctgacc atctatatag atccctccgg cggcgcggca aactatacgg gaataaaacc
      481 cccggacctc atcttcatca ccgacatcca cggcgaccac ttcgacctca acaccctctc
      541 ggccgttcgt acgtcttcta ccgcgatcat cgtccccaag gccgtcgccg acaaaatgcc
      601 cgactccctg aaaagcagga tcgtcatcct caacaatggc gataaaagct ctcagtccgg
      661 catcaccgtc accgccctcc ccatgtacaa tctccccgaa agccccaccg ccatgcatac
      721 aaagggccgt ggcaacggtt atgtactcgg catcggcggc aagaacctct acatctccgg
      781 cgatacacag ggtatccccg agatgcgggc ccttaaaaac atcgacatcg ccttcgtctg
      841 catgaacctc ccctatacca tggacgtcaa cgaagccgct gacgccgtcc tggccttcaa
      901 gccaaagacg gtctacccct accactaccg cggccagggt ggacttagcg acgtcaacgc
      961 cttcaaggca aaggtcgaag ccggtaacaa agacataaaa gtggtgctga aaaactggta
     1021 cccgacaaag ggctaaccaa acaaaggccc ccggcccaaa agcaaaagcc ggtccccaaa
     1081 aacaaaaaaa gccgccagaa aactggcggc ttaattattt ccggcgggtt tcaacaatgg
     1141 ctttcacgga taatgaacgc attgtggtct tcataagccg gatgtctttc ggtggatatt
     1201 ggatataaaa cttcggtttt gcaactcaaa gatgcgcggt tttacgccga tctcataaca
     1261 ttccattcac tattcgttga caatcatcca tagatttagg agaccctaag gaataccctt
     1321 atgaataggg aacttactat cgtattttat cgtaattata cgacacaact aattcacatt
     1381 gtggatattg gctagctcca taagctcgat cacattcttc gtccgggtcc gctcgaagat
     1441 cctcgacttc aaagtcgata ccgtacttac actcagattg agcgtgtcgg cgatctgcgt
     1501 cgtcttccac cccttcaata aataatgaag cacatccatt tcccgggccg gcatcccgga
     1561 aaagggcagg gcatcttctt cactgatgga gataggcgcc ataggacgtt gattcgtaag
     1621 aaattgccct atcatccgaa gcgtctccac ctcgttggcc gccttcgaaa taaaataacg
     1681 cacccggtag gggaacaagg cccgctcata taccgatatc ggctgcatgg aaaaaatgag
     1741 gatttcgagc tcagggtaaa gacccctgat cgtcgggatc acctccagcg tactcccgtc
     1801 ggaaagaaag atgtccagga tgagatgggt ataggtcttc gtctgcagct cgttcatcag
     1861 ctgattacag gtccacacct cctggacccg ctctttcccg atatggttct cgaccagcaa
     1921 cttcaatccc ttccgggtca tgctgtgatc gtcggcgatt aggagtttgt tttgcataat
     1981 aaagggaatc cattactact acatacacaa tgcgataata actacccact cccgccaggt
     2041 ctctgtcggt tttcccccaa atgttaataa aaaatccccg cactttcatg cagggatgcg
     2101 ctaatcagat gcattagcca tttaaacctt atcctattgg gagcaaaata atcccaattt
     2161 tacagtttcc ttgtagagat atttcgacaa gaccccggaa ttttttaaca ttcctgcctt
     2221 ttttagaaac atttacttta ttggtgatca attatttagt atattttacc ggtaatgttt
     2281 cgactctgga gaacactgaa gtacgccggc gccggcggtt tttatgaatc cgacatccgg
     2341 cgcgcagtct gcattggcgt gaacaccctg tgcgtgtcta tctttctgct gaacctcttc
     2401 gcgggactca tcttctttct gcttagcggt aaacttggta tcctcgtcgg cgcctacgta
     2461 gaaggtttcc tgatgctggg catcgtagca ctgaactatc gccgcaggta tgtccttgcc
     2521 aacatcttat tctacttcgt gatcaatctc gccaccttct atttcggcgc gatcctcggc
     2581 ccatatgccc aggtacagct gatgtttctc tttatccttg ggctcatctt ctttgtcttc
     2641 gacgacatcg ccggccgcat catatgtatc ctgatcaccc ttttgttatt gacattgctc
     2701 gagatccagt accggcaccc gttcatccag ccggcttcc
//
LOCUS       DATGFG010000678         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1069565, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000678 DATGFG010000000
VERSION     DATGFG010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069565"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VKQ52_12625"
     CDS             <1..430
                     /locus_tag="VKQ52_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88088.1"
                     /translation="VSQHGSQLSRLDEEITFLTDYLEMQKIRFGTALECAIHIPEENL
                     TNRYLLSFSLQPLLENAIKHNELTEETPLRVCVYQSGDRVIVSNNLQKRSVKTFSANH
                     GLANLAERYRLWSGDDIIIAEEENTFSVSIKLLTNEHSHH"
     gene            411..1175
                     /locus_tag="VKQ52_12630"
     CDS             411..1175
                     /locus_tag="VKQ52_12630"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ88089.1"
                     /translation="MNIVIIEDERVVAGDLEATLTQLVRGPVDITPLRSVKESIAWFK
                     ANDPPDLIFSDIQLGDGLSFEIFVATGITVPVIFCTAYDEYALEAFKANGIDYILKPF
                     TTQTLEQALQRYERLKRTFSTDQPSRYEALLQLMTDREAQRAASVLVHHQDKIIPIKV
                     EEIALFYLEKEVTRLLTFSGKTYFPNKNLDELEKLTGQYFFRANRQFLICRKAITDVS
                     NSFSRKLSINLSLPFKEKITISREKAPVFLDWLSKA"
     gene            complement(1231..>2185)
                     /locus_tag="VKQ52_12635"
     CDS             complement(1231..>2185)
                     /locus_tag="VKQ52_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88090.1"
                     /translation="EEDAFWTGERYCNPMAYFAQSNADYDGAEGALGKRCGIINADSG
                     SALITSGMIETDTNRVRVYKFLCSTLRKDKAFIWKGGVQYHHYSTNNRQGITPEEDSL
                     RWKLARVKSFTDALEPGVPCILGENGYDKSPHTRQSTPLLPGLGAEECQAIFILRSIN
                     ATAFSGFDSYILYWLKDTNPADDPNVYLTSGVLREMPDGSIHPYPSWFYLSTFESRLA
                     DYRPDAIVNEKGKVWVYKYRNVLSPDSVAYFLYLPTHNGSREPHYSLKVGDTDGGDAV
                     EVDFAPDTVEGKVTGQKVTGGMVSLGVEEKPKLILVKEKRK"
BASE COUNT          516 a          635 c          547 g          487 t
ORIGIN      
        1 cgtatcgcag catggttccc aattgtcgag actggatgag gaaataacct ttcttaccga
       61 ctacctcgag atgcaaaaga tccggttcgg cacggccctg gaatgtgcca tccacatccc
      121 cgaagaaaat ttgaccaaca ggtacctgct gtccttttcc ctgcagccgc tgctcgagaa
      181 cgcgatcaaa cacaacgaac tgacggaaga gacgccctta cgggtctgcg tttatcagtc
      241 gggcgaccgg gtcattgtgt ccaacaatct ccaaaagaga tcggtaaaga ccttttcggc
      301 caaccatgga ctggccaacc tcgcagagcg ctaccggctc tggtccgggg atgatattat
      361 cattgccgaa gaagaaaaca ccttttcggt gagcataaaa ctgttgacaa atgaacatag
      421 tcatcattga agacgaacgg gtggtcgccg gtgacctcga ggcaaccctt acccaactgg
      481 tcaggggacc cgtcgatatt acccccttgc gatccgtaaa ggaatcgatc gcctggttca
      541 aggccaatga tccccccgat cttattttca gcgatataca actcggagat ggtttgagtt
      601 ttgagatctt tgtggctacc gggatcaccg tacccgtgat cttctgcacc gcctatgacg
      661 aatatgccct ggaagcattc aaggcgaacg ggatcgacta tatcctgaaa ccctttacga
      721 cgcagaccct cgagcaggca ttacaacgat acgaaaggct gaaaaggaca ttctcgacgg
      781 atcagccatc ccggtatgaa gcgttgctgc agttgatgac cgaccgggag gcccaacggg
      841 cggcttcggt gctggtacac caccaggata agatcatccc gatcaaggtc gaagaaattg
      901 cccttttcta cctggagaaa gaggttaccc gtctcctcac cttttccggc aaaacctatt
      961 tccccaacaa gaacctggac gaactggaaa agttgaccgg ccagtacttc tttcgcgcca
     1021 accggcaatt cctgatttgc cgcaaggcca tcaccgacgt gtccaactct ttttcccgga
     1081 agctgagcat aaacctcagc ctgcctttca aggaaaagat caccatcagc cgggaaaagg
     1141 ctcctgtgtt cctggactgg ctgtccaagg cataagccgt ccagggcata gcgccatcca
     1201 ggcataaagc cgtcccgggc acaaacagga tcatttccgc ttttccttca ccaggatcaa
     1261 cttcggcttc tcctccaccc cgagcgatac catcccgccg gtcacctttt ggccggtgac
     1321 cttcccttcc acagtgtccg gtgcaaaatc gacttccacg gcgtcaccgc catcggtatc
     1381 ccccaccttc aatgaataat ggggctcccg ggagccattg tgggtaggca gatacagaaa
     1441 ataagcaacc gaatcgggcg aaaggacatt gcgatattta tagacccaca cctttccttt
     1501 ttcattgacg atcgcatcgg gccggtaatc ggccagccgg ctttcgaagg tgctgagata
     1561 gaaccaggag ggatacggat gaatgctccc atccggcatc tcccgcaata caccgctggt
     1621 caggtataca ttcgggtcgt cggcggggtt ggtgtccttg agccagtaga ggatatagga
     1681 atcgaacccg gaaaaggcgg tggcattgat ggagcgcagg ataaagatgg cctggcattc
     1741 ctccgcgccg agcccgggca ggaggggtgt cgactgtcgt gtatgcggcg acttgtcata
     1801 tccgttctct cccagtatac agggcacgcc aggctccagg gcatcggtga aacttttcac
     1861 cctcgccagt ttccatcgca gcgaatcctc ctccggggtg atcccctgcc ggttattggt
     1921 ggaataatga tgatattgca ccccgccttt ccagataaag gccttgtctt ttcgcagggt
     1981 actgcacagg aatttgtaca ccctgacacg gttggtatcc gtttcgatca tccccgaggt
     2041 gatcaacgcg ctgccgctgt ccgcgttgat gatcccgcac cgcttcccca atgcaccttc
     2101 cgcgccgtcg tagtcggcat tgctttgagc gaaataggcc atcgggttac aatatctttc
     2161 ccctgtccag aaggcatcct cttca
//
LOCUS       DATGFG010000679         3319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1290734, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000679 DATGFG010000000
VERSION     DATGFG010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290734"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VKQ52_12640"
     CDS             <1..531
                     /locus_tag="VKQ52_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLZ88091.1"
                     /translation="ISADFNYRTTLWKYGKHPREVMPHLLQYSSVAVADLDSAAVYYG
                     IETDKTASFEDRFRQCSTVLREKIPHLEVLAMSFRTPHGIQHSYSGALLYKGDNYFSV
                     RQELPFITDQIGSGDAFTAGMLYGLMRQLPPQQIIEYAVACGALKQSIAGDWALVTKS
                     EVEQFVQHGASGRIIR"
     gene            556..1617
                     /locus_tag="VKQ52_12645"
     CDS             556..1617
                     /locus_tag="VKQ52_12645"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HLZ88092.1"
                     /translation="MFIIDAHLDLSMNAMEWNRDLRLPVDDIRRREAGLTDKPDRARG
                     TVSLPDLRRGGIGLVVATQIGRFVAPGNPLPGWHSPQQAWAQTQGQLAWYKEMEAAGE
                     MVMIKDLSSLDEHVAGWDPSKPIGYILSLEGADSIVTPGYLQTAYEYGLRALGPAHYG
                     PGRYANGTDSSGGLNADGRALLREMERLGIILDATHLCDEAFWDAMELFHGPIWASHN
                     NCRVLVDHNRQFSDEMIRALIAKGAVIGGALDAWMMVPGWVRGQSNPLEMGCTLEKVI
                     DHLDHICQIAGNTLHVGMGTDLDGGFGKEQCPYDLETIADLQNMTTLLAKRGYSPEDI
                     GNILHGNWLRFLRKAWLPA"
     gene            complement(1614..2744)
                     /locus_tag="VKQ52_12650"
     CDS             complement(1614..2744)
                     /locus_tag="VKQ52_12650"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HLZ88093.1"
                     /translation="MRFLISTALLLLSFAGFSQQYYLFVGTYTEGTAGAGSKGIYVYT
                     FDAATGETKPVSTAATTNPSYLAIAPGGKFVYAVGETHGATPGSVSAFSFDKKTGQLT
                     FIDKQESGGADPCYVSVDAHRKWAFVANYSGGNFSALPIDANGSLQPATQTIQHTFSS
                     ADKRQDKAHVHSTILSPDEKYLVVCDLGMDKLSVLRFNAAAAKQPLSNAADSVVTLQP
                     GVGPRHSAFVPGKPYVYIIEELTGTVDAFHYSNGKLTPIQHISSLPDGFKGDIGSADI
                     HVSPNGKFLYASNRGDANNIVVYAIDPATGKLTVKGFQSTMGKTPRNFMIDPTGHWLL
                     AANQNGKNVVIFSIDQQTGLLTATGKQLEIPAPVCLKMTPMN"
     gene            2898..>3319
                     /locus_tag="VKQ52_12655"
     CDS             2898..>3319
                     /locus_tag="VKQ52_12655"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HLZ88094.1"
                     /translation="MRKINFLPAALLVGMIACNNADNKPAAGTDSTKTTDTGMAMAHM
                     DSTQAVPPLPTVPAGAKVYFKNLKDGQTVTSPVKVEMGIEGLKLDTAGAIVAGSGHHH
                     LLIDAGDSIPAGTVVPKDSTHLHFGKAQSTAEVKLTPG"
BASE COUNT          712 a          945 c         1002 g          660 t
ORIGIN      
        1 atctccgccg atttcaatta caggaccacc ctctggaagt atggtaagca tccccgtgag
       61 gtgatgcccc atctcctcca atacagcagc gtggcggtgg ccgacctcga ctccgccgcc
      121 gtctactatg ggatcgagac ggacaagaca gcgtccttcg aagaccgttt ccggcagtgc
      181 agtacggtgt tgcgggagaa gatcccgcac ctcgaggtgc tggcgatgag ctttcgcacc
      241 ccgcatggca tccagcacag ctattccggc gccttgctgt acaaaggcga taattacttt
      301 tccgtccgcc aggaattgcc gttcatcacc gaccagatcg gcagcggcga tgccttcacc
      361 gcggggatgt tatacggact gatgcggcag ttgccgccgc agcagatcat cgagtatgcc
      421 gtggcctgtg gcgcccttaa gcaaagtatc gccggcgact gggccctggt gacaaaatcc
      481 gaagtggaac agtttgtaca acacggcgct tcgggccgga ttatccgttg agacctaaaa
      541 ccccaacgac ttactatgtt tattatcgat gcacacctgg atctcagcat gaatgcgatg
      601 gaatggaacc gagaccttcg gttacccgta gacgatatcc gccggcgcga agcgggcctt
      661 accgataagc ccgaccgggc caggggaacg gtctcgctgc ccgacctccg caggggcggc
      721 atcggcctgg tcgtagccac ccagatcggg cgctttgtcg cgcccggaaa cccgcttccc
      781 ggctggcatt cgccacagca ggcctgggcg cagacccagg ggcagctggc ctggtacaag
      841 gaaatggagg cggcagggga gatggtgatg ataaaggacc tgtcttcgct ggacgaacac
      901 gtcgcgggct gggacccttc caaacctatc gggtacatcc tcagcctcga aggcgcggac
      961 tccatcgtga cacccggcta cctgcagacc gcttatgaat atgggctgag agccctgggc
     1021 ccggcgcact acggccccgg ccggtatgcc aatggaacgg acagctccgg cggtctgaac
     1081 gccgacggca gggccctgct gcgggagatg gagcggcttg gcatcatcct cgatgccacc
     1141 catctctgcg acgaggcgtt ctgggatgcg atggaactct ttcacggccc catttgggcc
     1201 agtcataaca actgtcgcgt gctggtggat cacaaccgcc agttcagcga tgagatgatc
     1261 agggcgctga tcgcaaaggg cgcggtcatc ggcggagcgc tcgatgcctg gatgatggtg
     1321 ccgggctggg tacgagggca gtccaacccg ctggagatgg gctgcaccct ggaaaaggtg
     1381 atcgatcatc tcgaccatat ctgccagatc gccggcaaca ccctgcacgt cggcatgggt
     1441 accgacctgg atggcggctt tggcaaggag cagtgtcctt atgacctgga gaccatcgcc
     1501 gatctgcaaa acatgaccac cctcctggcg aaaagaggct atagcccgga ggacatcggt
     1561 aatatccttc atggcaactg gcttcgcttc ctgcgcaaag cctggctacc cgcttaattc
     1621 atcggtgtca tcttcagaca aacgggcgcg gggatctcga gctgtttgcc cgtggccgtc
     1681 aacagaccgg tttgctggtc gatggaaaag atcaccacat tcttcccatt ctggttggca
     1741 gccagcagcc agtggcccgt cgggtcgatc atgaaattgc ggggcgtctt tcccatcgta
     1801 gactggaatc ctttcaccgt cagcttcccc gtggcgggat cgatggcata gaccacgatg
     1861 ttgttggcat ccccgcggtt ggaagcatag aggaatttcc cattgggcga cacgtggata
     1921 tcggcgctgc cgatatcgcc cttgaatccg tccggcaggg aagagatatg ttgtatgggt
     1981 gtcagcttcc cgttgctgta gtggaaagcg tcgacggtgc cggtaagctc ctcaatgata
     2041 tagacgtagg gcttgccggg gacaaaggcc gaatgacgcg gacctacccc gggttgcagg
     2101 gtgacgacgg aatcggcggc gttgctgagc ggctgttttg cggcagcggc gttgaaacgc
     2161 aggacggaga gcttgtccat ccccagatcg cagacgacga gatatttttc atccggcgaa
     2221 agaatggtgg agtgaacatg cgctttatcc tggcgcttgt cggcgctgct gaaggtatgc
     2281 tggatggtct gtgtcgcggg ctgcagggag ccattggcgt cgatgggcag cgcggagaaa
     2341 ttaccgccgc tgtaattggc cacgaaggcc cacttgcggt gggcatcaac cgaaacataa
     2401 caggggtcgg cgccgccgct ctcctgcttg tcgataaagg tcagctgccc cgtcttctta
     2461 tcgaaggaaa aagcgctgac gctgcccggt gtggccccat gcgtttcccc gacggcatac
     2521 acgaacttcc cgcccggggc gatggccaga taggagggat tggtcgtggc ggccgtgctc
     2581 accggttttg tctcgcccgt ggcggcatcg aaggtataga catagatgcc cttgctgccg
     2641 gcgccggcag taccttccgt ataggttccg acaaacagat agtattgctg cgagaaaccc
     2701 gcaaaggaca gcagcaggag ggcggtggaa ataagaaaac gcatccggtg attgttgatt
     2761 ttgtggaaga aatgattaca cgcaagataa ggaaaagaaa gaatccgatt ttactcccgc
     2821 ccgggggttt aacccgctcc ttttgggtca tatcctcaga ttaagtactt ttattcacaa
     2881 aataaactac ttacatcatg cgcaagatca attttcttcc cgccgccctg ttggtgggaa
     2941 tgattgcctg caataacgcg gacaacaaac ctgcggccgg tacggactcg acaaagacaa
     3001 ccgatacggg gatggcgatg gctcatatgg attcgactca ggcagtgcct cccttaccca
     3061 cggtgccggc aggagccaag gtgtatttca aaaatctcaa ggacggtcag acggtgacct
     3121 cgccggtgaa ggtagagatg ggcatcgaag ggctgaagct ggatacggca ggcgcgatcg
     3181 tcgccggaag cggtcatcac cacctgctga tcgatgccgg agactccatc cccgccggaa
     3241 cggttgtgcc caaggactcc acccacctcc attttggtaa ggcgcagtcc accgccgaag
     3301 tcaaactgac ccccggaaa
//
LOCUS       DATGFG010000680         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1159091, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000680 DATGFG010000000
VERSION     DATGFG010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159091"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1449
                     /locus_tag="VKQ52_12660"
     CDS             214..1449
                     /locus_tag="VKQ52_12660"
                     /inference="COORDINATES: protein motif:HMM:NF040152.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HLZ88095.1"
                     /translation="MDYRSILEQAQHYVRALFDTHVNAKLHYHNLRHTELVVEAVTQI
                     AQHYQLSDVDFFTVCVAGWFHDIGYLTGEPQGHEERGARMAETYLAGTGVEHGVIEDV
                     KRCIIATQLPQRAVGLTEQIVCDADLFHLGTDAFSERNKLMRKEAEDLQSRKISKEEW
                     RRSTIRLLESHQYYTDYCRLLLNEKQLENLMKLKAKEPDDSGNAIDTLLREHGVEEDE
                     EGNLQLTEKNRKDKKKDPGRGIDTVFRITSNNNQKLSSQADSKAHILIQVNAIIISVL
                     LSLLLRKIEDHTNLAIPATMLLTVNLITIVFSILATRPHIPAGTFSQADLDEKKVNLL
                     FFGNFYRMSLEQYANGMLIMMEDKDFLYGSLIRDVYFQGIALGKKYRWLRLSYNVFMY
                     GLIASVAAFLIAALAFPSK"
     gene            1465..>3533
                     /locus_tag="VKQ52_12665"
     CDS             1465..>3533
                     /locus_tag="VKQ52_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88096.1"
                     /translation="MQNAVIHIIKELEGAGEVNSRLSFDGFIQFLKKRRLEDKTMRVK
                     FLDYVIHYFEERLQGKHVIELEEMSLYADLLELVYTVIFPVLADERDTAWALGVPVKP
                     IVFYGTDPFYDKLRDPASKKIRACMIDEQNQERTKVNLELIYSMILRQCYQYELPAGN
                     TMIRSMRNEETGLPGFYRLNIDSRFIRILPNGELPAFDPAALQPHRPVKETVAWLLAH
                     LPLNAFVFEGFSALTVTDITEEYVMESVKGLILNPDLCDGESHHGEVLRYLNILAGTN
                     GVSFGLLPLLKVNGRPVYAEEICQDSVLGKAAGSDEETEETYLCLVNRYFKEPRQLLY
                     ETLPGREEGEYFFLETLRRGGIVSYGLVPVYYNNRLAGVLEASTREPGILNAELLSRL
                     DVVLPLLAQLMQRGIDEFDAELKAIIKENFTSIQPAVEWKFNEAAWHYARLRERGDVP
                     ASIETIYFKDVYPLYGAIDIRNSTIERNSALRRDLHTQFSMLVETLSALQKSANLQLL
                     EELLHQTQKWQQAMNGALTTADELNLNSFLRDKVDSFLVHFRETRPDLADLITPYLQA
                     TEETEGEAFRHRRELESCIQLVNKTINGLLEETIAELQQSYPFYFEKFRTDGVEYDIY
                     VGESISPDRTFDLLYLRNLRLWQLKSMAMVARRTHSLLPEMVDPLETTQLIFVHSAAI
                     DISFRRD"
BASE COUNT          787 a          802 c         1138 g          806 t
ORIGIN      
        1 ctagggtggg gaagggtgag gttggagcgg ggaatgatgg gacggtgagg ttggtgcggc
       61 cgaggcgaag tttggagcgg ggaagttggg cgcggcgagg ctggagcgga cgagatgccg
      121 ggggtggaaa agcctactgt ccattcggcg gcaattgatg tctaattcta cagaattcaa
      181 cccaaaaccc tgattatttg ttaatttgta tttatggatt atcgaagcat attagaacag
      241 gcacagcatt atgtaagggc gttgtttgac acgcatgtca atgcgaaatt acattaccat
      301 aacctgcggc atacggagct ggtggtggag gcggtgacgc agatcgcgca gcattatcaa
      361 ttgtcggatg tggatttttt taccgtgtgt gtggccggtt ggtttcatga tatcgggtat
      421 cttaccggcg agccccaggg gcatgaggag cggggggcga ggatggcgga gacctatctc
      481 gccggtacgg gagtggagca tggggtgatc gaggatgtga agcggtgcat catcgccacg
      541 caattgccgc agcgggccgt ggggctgacc gaacagatcg tttgtgatgc ggatcttttt
      601 catctgggca ccgatgcttt cagcgagagg aacaagctga tgcggaaaga ggccgaggat
      661 ctgcagagcc ggaagatcag caaggaagag tggcgccgga gcaccatccg cctgctggaa
      721 tcgcaccagt attataccga ttactgccgg ctgttgttga atgaaaaaca gcttgagaac
      781 ctgatgaagc tgaaggcgaa ggagccggat gattcgggga atgcgataga tactctctta
      841 cgggagcatg gcgtcgagga ggatgaggag ggcaacctgc aactgacgga gaagaacagg
      901 aaggacaaga aaaaggaccc gggcaggggg atcgacacgg tctttcggat cacctccaac
      961 aataaccaga agttgagttc gcaggccgac agcaaggcgc atattctcat ccaggtgaac
     1021 gcgatcatca tttccgtcct gctcagcctg ttgctccgga agatagagga tcataccaat
     1081 cttgccattc cggcgaccat gctgctgacg gtgaacctga taacgatcgt cttttcgatc
     1141 cttgcgaccc ggccgcatat cccggcgggg acctttagcc aggcggatct cgatgagaag
     1201 aaggtgaacc tgctgttttt cgggaatttc taccggatga gtctcgagca gtatgccaac
     1261 gggatgctga tcatgatgga ggacaaggat ttcctgtatg gcagcctgat ccgggatgtt
     1321 tatttccagg ggatcgcgct tgggaagaaa taccggtggc tgcggctttc gtataatgtc
     1381 tttatgtatg ggcttatagc gtcggtggcg gctttcctga tcgctgcgct tgcttttccg
     1441 tccaaatgaa aaatgtttta gggtatgcag aacgcagtca tccatatcat caaagagctg
     1501 gagggggcgg gagaggtgaa ttcccggctt tccttcgacg gatttattca attcctgaag
     1561 aaacgcaggc tggaggataa gaccatgcgg gtcaaattcc tggattatgt catccattat
     1621 ttcgaggagc ggttgcaggg aaagcatgtc atcgaactgg aggagatgag cctgtatgcc
     1681 gaccttctcg agctggtgta taccgtcatt ttccccgtgt tggccgatga gcgggatacg
     1741 gcctgggcgc ttggggtccc ggtgaagccc attgtttttt atggcaccga tccattttat
     1801 gacaagctgc gggacccggc gtcgaagaag atcagggcct gtatgatcga cgagcagaac
     1861 caggagcgga ccaaggtgaa cctggagctg atctattcca tgatcttacg ccagtgctat
     1921 caatatgagc ttccggcggg caatacgatg atccggagca tgcgtaacga ggagacgggg
     1981 ttacccggct tttaccggct caatatcgac agccggttta tccggatcct gccgaatggg
     2041 gagcttccgg ccttcgaccc tgcggcgttg cagccgcatc ggccggtgaa ggagacggtc
     2101 gcatggcttc tggcgcatct gccgctgaat gcgttcgtat tcgaagggtt ttcggcgctt
     2161 acggtgacgg acatcacgga ggagtatgtg atggaaagtg tcaaggggct gatcctcaac
     2221 ccggaccttt gtgacggcga gagccatcat ggggaggtgc tccggtatct gaacatcctt
     2281 gccggaacga atggagtgag ttttgggctg ttgccgctgc tgaaggtgaa cggcagaccc
     2341 gtgtatgccg aggagatctg ccaggacagc gtccttggga aggcggccgg gagcgatgaa
     2401 gaaacagagg agacgtatct atgcctggtg aaccggtatt tcaaggaacc aaggcagctt
     2461 ttatatgaga cgcttcccgg gcgggaagag ggggaatatt tcttcctgga gacgttgcgg
     2521 cgtggcggca tcgtttctta tggattggtg cccgtgtatt ataacaaccg gcttgccggg
     2581 gttttggagg cttctacccg ggagccgggg atcctgaatg ccgaactgtt gtcccggctg
     2641 gatgtggtgc tcccgttgct tgctcaactg atgcagcggg ggatcgatga gttcgatgcg
     2701 gaactgaagg cgatcatcaa ggagaatttt acttctattc agccggcggt ggaatggaaa
     2761 ttcaatgagg cggcctggca ttatgcgcgg ctgcgggaac ggggggatgt accggcgtcg
     2821 atagagacca tctattttaa agacgtctat cctttatatg gggccatcga tatccggaac
     2881 tcgacgatag agcggaacag tgccttgcgc cgggatctgc acacccagtt cagcatgctg
     2941 gtggagacct tgtcggcatt gcaaaagtcg gcgaacctgc agttgctgga agagctgctg
     3001 caccagaccc agaaatggca acaggcgatg aacggtgcgc tgaccacggc cgatgagctg
     3061 aacctgaaca gttttctacg ggataaggtg gattccttcc tcgtgcattt ccgggagacg
     3121 aggcccgatc ttgccgatct tatcacgcct tatctgcagg cgacggagga gacggaaggg
     3181 gaggcgttcc ggcaccggcg cgagctggag agctgtatcc agctcgtcaa caagacgatc
     3241 aatgggctgc tggaggagac gattgccgaa ttgcagcagt cctatccttt ttattttgag
     3301 aagttccgga cggatggggt ggagtatgat atctatgtcg gcgagtcgat ctctcccgac
     3361 cggaccttcg atcttttgta tctgcggaat ctccggctct ggcaattgaa gtcgatggcg
     3421 atggtggccc ggaggacgca tagcctgttg ccggagatgg tcgatccttt ggagaccacg
     3481 caacttattt ttgtgcactc ggcggcgatt gatatcagct tccggaggga cga
//
LOCUS       DATGFG010000681         4808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_846836, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000681 DATGFG010000000
VERSION     DATGFG010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846836"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..646)
                     /locus_tag="VKQ52_12670"
     CDS             complement(101..646)
                     /locus_tag="VKQ52_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88097.1"
                     /translation="MRFDRVGWSAKMFSVCLIGGGEWSSSRCRLTWKLQGTRYGRIFC
                     RLRVSGRRTEGSGCGLLPTPVAMDAEDIRQLRRSTIEGMKRGSMASMTLTHYLAVGFL
                     PTPTARDGKGGFRAGSYAFLKRQEHARGVNLHEELQRRTGKDFQLSPLFVLEMMGFPP
                     DWTALPFLPGAINPFAGQVTP"
     gene            complement(978..1568)
                     /locus_tag="VKQ52_12675"
     CDS             complement(978..1568)
                     /locus_tag="VKQ52_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88098.1"
                     /translation="MHIQLKNVRVNEGLSRESLAFTATLYINGLRVGRITNDGNGGET
                     NYEPLDSRGASIIKEAVKWVGSFAPKLGSDDVDNRDLEPDRSKFGFHLEDAATGWWNG
                     REAARFHKKAEKAMENGILFGVPGQAYRVMKYRQSIATIIELKKGVEQLKQDIHTKVM
                     PLLNESEKILNTNIPREIIGLLAVPDGKWVDQPGGK"
     gene            complement(1592..2371)
                     /locus_tag="VKQ52_12680"
     CDS             complement(1592..2371)
                     /locus_tag="VKQ52_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HLZ88099.1"
                     /translation="MDRNDVLMISFSGGRSSAYMTDRLLREGWKGEVVICFANTGKEA
                     AETLDFVHACELSWQRKVHWLEYDLVTRFREVEYETASRKGEPFAALIDKRKYLPNVV
                     WRFCTQDLKVRVIKRFMMSLGYKHWINAIGIRYDEPARWSKTRAIGERERWETWLPLV
                     DWRITKPIVLDFWRTMPFDLQLEHYQGNCDLCFLKGRNKMRRLLLEDPALGDWWMEQE
                     DKTGATFRKGLSVRRFVELIRASPSLFEYEDADIECFCNVD"
     gene            complement(2389..2910)
                     /locus_tag="VKQ52_12685"
     CDS             complement(2389..2910)
                     /locus_tag="VKQ52_12685"
                     /inference="COORDINATES: protein motif:HMM:NF016921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HLZ88100.1"
                     /translation="METVKETNRRGLKYSVVYADVPWGEENWFLDRIRELPVINLVEE
                     NALLLLWVPASLVPQGLLVLGEWGFEYAGLLTWRKSPDRVERFPAHSICEYMLVGKLG
                     SVQTDKVLRNMLYEGPETSASYKPQEFRSIFGGAGRYLFGELATFLDVFGKYWQGRYA
                     DYETEIWDFLYDE"
     gene            complement(3022..3921)
                     /locus_tag="VKQ52_12690"
     CDS             complement(3022..3921)
                     /locus_tag="VKQ52_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4138 domain-containing protein"
                     /protein_id="HLZ88101.1"
                     /translation="MKERILKGTVRRRWLLGAMRDALCMGRKAVMGMWTVVVGLSVHA
                     QEPGFHLEGMTSRVLPVTGRKMTNVIFPVDIAAGVRVSRDVLVQKVRGVENVIELKAL
                     KRDFQPTSLAVYGKDGRLYSFVLHYVEDTTVLDYRIVPDGGAAEPKVRLAGWRVEPER
                     LRKDARMLAKRRGSLHQKANGDGLRLVLKGVNLRDSLLWLSLELEDRTAMGCGPGDLR
                     IYAEDRKRVNRTASQEVEISPVYAEGIAALPGLGRQATAVGLRPFVIGRGKRLVVTYS
                     DEADDRQVVLKVKRKWLLRARNG"
     gene            complement(3931..>4808)
                     /gene="traM"
                     /locus_tag="VKQ52_12695"
     CDS             complement(3931..>4808)
                     /gene="traM"
                     /locus_tag="VKQ52_12695"
                     /inference="COORDINATES: protein motif:HMM:NF023922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugative transposon protein TraM"
                     /protein_id="HLZ88102.1"
                     /translation="DLLRKLDQLKGVLSRREQAAIPEGPLAAGVPEKPVFPGSGLPVT
                     PSVPFASALARGEQGDPDLDKLNTLMDKVLKVRYPGDAPLRDSAPVLRDERSVQVLAA
                     PIQEEVMSTLPNDEKDDLETGFIDLDDGARTDSLAESMIAAVVDGSQTLISGEAVGLR
                     TAGDAMLGGIRVPRGTSLSGKATVSGERLLVAVSAVRINSRVIPVSLEVVDMDGMAGI
                     RVKGSLNRDVSKESASEAVGSLGVTSLDPGVAGQATAAGIQAAKNLLGRKIRLVRVGL
                     PAGYRVLLRNNKVNR"
BASE COUNT         1076 a         1710 c          930 g         1092 t
ORIGIN      
        1 ccatgaaatc cgttatttcc cgcgcttctt tcatttcttt atcctccata tttctccatc
       61 cgcccgatcg ttcggaatag ctgcaacacc acccgcggta ctacggcgtt acctgccccg
      121 cgaagggatt gatagcgcca ggaagaaaag gcaatgccgt ccaatccggc ggaaatccca
      181 tcatctccaa cacaaaaagg ggactcagtt ggaaatcctt cccagtccgt cgttgcagtt
      241 cctcgtgcag gttcactccc ctcgcatgct cctgccgctt caaaaaagca tacgaaccgg
      301 cccggaaacc acccttccca tcccgggccg tcggcgtcgg caggaatccc accgccaggt
      361 aatgcgttaa cgtcatgctc gccatggatc cccgcttcat gccttcgatg gtcgacctcc
      421 gcaactgccg gatgtcttcc gcatccatcg ccaccggcgt gggcaacaat ccacacccgc
      481 tcccttcggt gcggcgcccc gacacccgca gccggcagaa tatacgccca tacctcgtac
      541 cctgcagctt ccaggtcagt ctgcacctgc tcgaagacca ttcccctccg ccaattaagc
      601 agaccgagaa cattttcgcc gaccacccaa cgcggtcgaa cctcattaat gattcgacac
      661 atctccggcc ataagtagcg gtcatccgac gttccctttc gctttccggc gacgctgaac
      721 ggctgacaag gaaagccgcc ggaaagtacg tcaataactc ctctatagac agttgcgtcg
      781 aattccctga cattcccata caatttcgta ttaggccaat aaaaccgcaa tacccgctgg
      841 caaaagtcct ccttctcgca ctggaacaca ttctcccaac ccatccacgt cgccgccagg
      901 tcgaacccgc cgattccgct aaacaaactc ccatgtctca tacctttccg ctcatttccc
      961 ctctcgcctc ttgcccttta tttcccccct ggctgatcca cccattttcc atccggcact
     1021 gccaacagcc caatgatctc ccggggtata ttagtattga gtatcttttc gctctcgttc
     1081 agcaatggca tcaccttagt gtgaatatcc tgtttaagct gctccacccc cttctttaac
     1141 tcaattatgg tggcaatcga ctgtctatat ttcattaccc ggtacgcctg cccgggaacc
     1201 ccgaacaaaa tgccgttctc cattgctttt tcagccttct tgtgaaatct cgcagcttcg
     1261 cgaccgttcc accaacctgt cgccgcgtcc tcgagatgaa acccgaactt gctccggtcc
     1321 ggttccaggt ccctattgtc aacatcatcg gaaccaagtt tcggggcaaa gcttccgacc
     1381 cattttactg cttctttaat tatcgatgcg ccacgactat ccaatggctc ataatttgtc
     1441 tcaccgccat taccatcatt tgtgatgcgc ccgaccctca gcccattaat ataaagagta
     1501 gcagtaaatg ccaaagactc ccgactcagg ccttcattca cgcgaacatt tttcaattgt
     1561 atgtgcatga ctcccatttt acgttgttac tctaatccac attgcaaaag cactcgatat
     1621 ccgcgtcctc atactcgaac agcgaaggcg aggccctgat cagttcaacg aaccgccgta
     1681 ctgacaatcc tttccgaaag gtcgcacccg tcttgtcttc ctgttccatc caccaatccc
     1741 ccagcgccgg atcttccagc agcaaccgcc gcatcttgtt ccgccctttc aggaaacaca
     1801 ggtcgcaatt gccttgatag tgctccagtt gcagatcaaa cggcatggtc cgccagaaat
     1861 ccagtacgat gggcttcgtg atccgccagt ccaccagcgg cagccaagtc tcccatcgct
     1921 cccgttcgcc gatggcccgc gtcttcgacc atctcgccgg ctcatcgtaa cggatgccga
     1981 tggcattgat ccaatgctta taacccaggc tcatcatgaa ccgcttgatc acccgcacct
     2041 tcagatcctg ggtacaaaag cgccagacca cattcggcag gtattttcgc ttatcgatca
     2101 atgccgcgaa aggctcgcct ttccggcttg cggtctcata ttccacctcc ctgaacctgg
     2161 ttaccagatc atattcaagc cagtgtacct tccgctgcca agacagctcg caagcatgca
     2221 caaaatccaa ggtctccgcc gcttctttgc ccgtattggc aaaacagatc actacttctc
     2281 ccttccaacc ctccctcagc aaacggtccg tcatatatgc ggaactccgt cccccgctga
     2341 aactgatcat cagcacatca ttcctgtcca taccccgcat ctgtttactt actcatcata
     2401 caaaaagtcc catatctccg tctcataatc cgcataacgc ccctgccaat acttcccgaa
     2461 cacatcgaga aaggtcgcca actcgccaaa caaataccgc ccggctcccc cgaaaattga
     2521 cctgaattcc tggggtttat agctggcgga agtttccggt ccttcataga gcatattccg
     2581 cagaacttta tcggtctgaa ccgatcccag ttttcccacc aacatatatt cgcatatgct
     2641 atgggccggg aacctttcca cccggtccgg ggacttcctc caggtaagta atcccgcata
     2701 ctcgaagccc cattccccaa ggactaacag tccctgcggt accagggagg caggaaccca
     2761 caacaaaagc aaggcattct cctcgaccaa attaattaca ggtagttccc tgatcctgtc
     2821 aagaaaccaa ttctcttcac cccaaggcac atctgcataa acgaccgagt attttaatcc
     2881 ccgccgattc gtttctttta ctgtttccat ttcttttatt ttaattttaa tttaaatacc
     2941 agcttcacca ctttgagtgc caccgttgcg tcgcaatcac ttcatcggca aaccccctta
     3001 tcatcactcc aacaaaagcc gctacccatt ccgcgcccgg agcaaccatt ttcgcttcac
     3061 cttcagcacc acctgccggt catcggcctc atccgaatat gtaacaacca gccgttttcc
     3121 tcgtccaatc acgaaaggac gcaaccccac cgctgtcgcc tgccgtccga gccccggcaa
     3181 agccgcaatc ccttctgcat acaccggcga aatttccacc tcctgagacg ccgttcgatt
     3241 taccctcttc cggtcttccg cataaatccg aaggtcacca ggtccgcatc ccatcgccgt
     3301 cctgtcctcc aactccaacg acagccaaag aagactatcc cgcaggttaa caccttttag
     3361 caccaaccga agcccatccc cgttcgcctt ttgatgcaga gagccacggc gctttgccag
     3421 catccgcgca tccttccgta accgctccgg ctctacccgc caaccagcca gtcgaacctt
     3481 cggttcggct gcccccccat cgggcacaat ccggtaatcc aagaccgtcg tatcttccac
     3541 ataatggaga acgaaggaat acagccttcc atcctttcca tacaccgcca ggctcgtcgg
     3601 ctgaaaatcg cgcttcaagg ccttcagttc tataacattc tccacccctc tcaccttttg
     3661 caccagcaca tcccggctta cccgcacgcc cgcagctata tccaccggga aaatgacatt
     3721 cgtcattttc cggcccgtca cgggcaagac acgactggtc attccctcca ggtgaaaccc
     3781 cggctcctgc gcgtgaaccg acagacctac aaccactgtc cacatgccca tcactgcctt
     3841 ccggcccata cacagcgcat cccgcattgc ccccagcaac caccgtcgcc gaacggtccc
     3901 tttcaatatc ctttccttca tatcctgtcc ttaacggtta actttattat tcctcaacaa
     3961 taccctgtac cccgccggta aacccacccg caccagacga atcttccgac ccagcaggtt
     4021 cttcgcggcc tgaatacccg ccgccgtggc ctgtccggcc acgccggggt ccagcgaagt
     4081 aacacccagc gatcccacgg cttcactggc cgattccttc gacacgtccc tgtttaaaga
     4141 gcctttcacc cgaatgccgg ccatcccatc catatccacc acttccagcg atacagggat
     4201 cacccggctg tttattcgaa ccgcacttac tgccaccagc aggcgttcgc ccgacaccgt
     4261 cgccttgcca gacagggacg tacccctggg tactcgaata ccgcccagca ttgcatcacc
     4321 ggccgtacgc aaaccaaccg cctcgcccga tatcagcgtc tgcgagccgt ccaccaccgc
     4381 cgcgatcata ctctccgcca gcgagtcagt acgagcccca tcgtccaggt cgataaatcc
     4441 cgtctccagg tcgtccttct catcattcgg caaggtgctc atcacttcct cctgtatggg
     4501 cgccgccaat acttgcaccg accgctcatc ccgcaacaca ggcgccgaat cgcgcaatgg
     4561 cgcgtcacca ggataccgca ccttgagcac cttatccatc agcgtattca acttgtccag
     4621 gtccgggtcg ccttgttctc ccctggccag tgcactggca aagggtacgg aaggagttac
     4681 cggcaaaccg ctacccggaa acactggttt ttcgggcacg cccgccgcaa gcggcccttc
     4741 cggtatcgcc gcttgttccc gccgactcag cacacccttt agctggtcga gctttcgcag
     4801 caggtcac
//
LOCUS       DATGFG010000682         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_62779, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000682 DATGFG010000000
VERSION     DATGFG010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62779"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1346
                     /locus_tag="VKQ52_12700"
     CDS             144..1346
                     /locus_tag="VKQ52_12700"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ88103.1"
                     /translation="MITIGLLREGKIPADNRVALTPAQCKWIHKNAPQVRVIVQASAD
                     RCYTDKEYGNAGIEVREDIGGCDILFGIKEVPAKDLIPDKTYLFFSHTKKQQPVNRRM
                     FRTILERNITLIDYECLEHDDGQRILGFGFFAGVVGAHNGIMAYGKRTGGYDLGRVHK
                     QKSFRELIHTYFGLRLPEIKVAVTGSGRVAHGVLEVMNLLEIVEVEPDEFLERRFSYP
                     VFTQLKGSVLYEHRQTKTYHRDHFHRHPEEYRCKFLPFTRTTDILMNGIYWDKNMPRL
                     FEWEDLAQPDFHISTIADITDDREGSIPCNLGESSIDDPIYGVDRQTRQRTAPYLPGS
                     VDIMAVGNLPNELPRDASRYFGEQLIKHVLEPLIRGGSKIIERATMVRAGKLTEPFHY
                     LAEYAAGG"
     gene            complement(1412..2128)
                     /locus_tag="VKQ52_12705"
     CDS             complement(1412..2128)
                     /locus_tag="VKQ52_12705"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLZ88104.1"
                     /translation="MPNPYFQFKQFMIRQDQCAMKVCTDACILGAWFANKTPAWSQVL
                     DIGSGTGVLMLMLAQKHKGEIRGIELDLNAYRQLKDNIGQSPWRQMLKVFPGDVRSFS
                     FPGKFDFIITNPPFFEGDLPAASEAANLARHSKELTLAELLTVIDANLSPEGSFGILL
                     PYHRSAWFEELAGSAHGFSLREKLLVRQTPRHDFFRSILYFSRRREGFIPTTELTIQN
                     NEGVYTEDFTELMRDYYLYL"
     gene            complement(2146..3216)
                     /gene="tsaD"
                     /locus_tag="VKQ52_12710"
     CDS             complement(2146..3216)
                     /gene="tsaD"
                     /locus_tag="VKQ52_12710"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HLZ88105.1"
                     /translation="MQAPNAGPVTLLAIESSCDETSCAISRDGAILANIIAGQAVHEQ
                     YGGVVPELASRAHMQNIVPVVDAALKKAGVSLSELNAIAFTQSPGLIGSLLVGSQFAK
                     SLAQALDIPLVSVHHMQAHVMANLIAPAGAATGPSGTTDSADAGTPSFPFLCLTVSGG
                     HTQIVLCHSPLKMEVIGETLDDAAGEAFDKSAKLLGLPYPGGPLIDKYARTGDATRFS
                     FPEPQIPGLNFSFSGLKTAILYFLADQESKDPGFVSANLPDICASIQGRIVSILLNKL
                     RRAARETGVTDLCIAGGVSANSGLRKAFGEMGKKEGWNTFIPPFEYCTDNAAMIAITG
                     YYKYLAGEFAPLSTSASARAEW"
     gene            complement(3262..>3993)
                     /locus_tag="VKQ52_12715"
     CDS             complement(3262..>3993)
                     /locus_tag="VKQ52_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ88106.1"
                     /translation="FNKPEKAIGQTVKLKGGRNAIVIGVIEKQGQSLIGGWDYDNCIL
                     LTYAFMKQMIREENAQPKLLAQAGANISTEALSDELRGAMRSIRKLSPTQEDNFALNN
                     IDTLTKFFDPIFRGMNIGGWAIAALSLIVGMFGVANIMFVTVRERTSQIGLKKAIGAK
                     RGVILTEFLLESAFLCIIGGLIGLVAVFILTLIFSAILRFQVIIPLNIILLAVSICLF
                     TGVAAGIIPAFVAARMDPVVAIRSK"
BASE COUNT          914 a         1137 c         1157 g          785 t
ORIGIN      
        1 agttccgcca tctgggcatc gtagcacgca ctatcgcgga aaaaccgcgg gaaaagcggg
       61 acgcaatatt acggatttgg gctgttctgt ccaaaaagga ataaaaaatt ctatcttagc
      121 cgctaacaga taaggaaata aggatgataa cgatcggctt gttacgtgaa ggcaaaattc
      181 ccgcagacaa cagggtggcg ctgactccag cccaatgcaa atggatccat aagaacgcgc
      241 cgcaggtgcg ggtgatcgta caggcttcgg cagaccgctg ctataccgat aaggagtacg
      301 gcaatgccgg catcgaagta cgggaagaca tcggcggctg tgatatcctc ttcggcataa
      361 aagaagtccc ggcaaaggac ctgattcccg acaagaccta tctctttttc tcccatacaa
      421 aaaaacaaca gcccgtcaac cgccggatgt tccggaccat cctggaaagg aatatcaccc
      481 tgatcgatta cgaatgcctg gaacatgacg atggtcagcg catcctcggt tttgggttct
      541 tcgccggggt cgtcggcgcc cacaacggca tcatggccta cgggaagcgc accgggggct
      601 atgacctcgg gcgggtacac aagcagaaaa gcttccgcga actgatccac acctatttcg
      661 ggctgcggct gccggagata aaggtcgcgg tcaccggctc cggccgcgtg gcgcacggcg
      721 tactggaggt gatgaacctc cttgaaatag tagaggtcga gcccgacgaa ttcctcgaac
      781 gccggttctc ctatccggtg ttcacccagc taaaaggcag tgtattgtat gaacatcgcc
      841 aaacaaagac ctaccatcgc gatcactttc atcgacaccc ggaagaatac cgctgcaagt
      901 tcctgccgtt tacccggacg acggatatcc tgatgaacgg gatctactgg gataagaata
      961 tgccccggct tttcgaatgg gaagacctcg cccagccgga tttccacatc agcaccatcg
     1021 ccgatatcac cgatgaccgc gaggggtcga tcccttgcaa cctgggcgag tcctccatcg
     1081 atgacccgat ctatggtgtg gaccggcaga cgcggcagcg cacggcgccg tatctccccg
     1141 gatcggtcga tatcatggcc gtcggcaatc tgccgaacga actgccaaga gatgcctcgc
     1201 ggtattttgg cgagcagctg atcaaacatg tgttggaacc cctgatcagg ggcgggtcga
     1261 agatcatcga aagggcgacg atggtgcggg cgggaaagct gaccgaacca tttcactatc
     1321 tggccgaata cgcggccggc ggataaacgc gcggcggccg gcggcaagag ccaggcccaa
     1381 atctgcggca aaaccacggc cgcgcctgcg tctataaata caaataataa tcccgcatca
     1441 gctcggtaaa atcctccgta tataccccct cgttgttttg tatcgtcagc tccgtcgtcg
     1501 gaataaaccc ttcccggcga cgcgagaaat aaaggatact ccggaagaaa tcatggcggg
     1561 gcgtctgcct caccagcaat ttttcgcgca gggaaaaacc atgggccgag ccggccaatt
     1621 cctcaaacca tgccgaccgg tgataaggaa gcaggatacc gaaagaacct tccggtgaca
     1681 ggttcgcatc gatcaccgtc agcagctcgg ccagcgtcag ttccttgctg tggcgcgcaa
     1741 gattggcggc ttcggaagcg gcaggcagat ccccttcgaa gaagggcggg ttggtgatga
     1801 taaaatcgaa tttgccggga aagctgaagg aacggacatc gccgggaaaa acttttaaca
     1861 tctgcctcca ggggctctgg ccgatattat cctttaattg ccggtaagca ttcagatcga
     1921 gttcgatgcc cctgatctcg cccttatgtt tctgcgccag catgagcatg agcacgccgg
     1981 tcccgctgcc gatgtccaga acctgcgacc aggccggcgt cttgttggcg aaccaggcgc
     2041 cgaggataca ggcatccgtg caaaccttca tggcgcattg atcctgccgg atcatgaact
     2101 gcttgaattg aaaataaggg tttggcatgg cgcaatatat tttttttacc actctgcccg
     2161 cgcgctggcg cttgtcgaca gcggggcaaa ttcgcctgca agatatttgt agtaaccggt
     2221 gatggcgatc atcgccgcgt tgtccgtgca atattcaaag ggcgggataa aggtattcca
     2281 gccttccttt ttgcccatct ccccgaatgc cttgcgcaac ccgctgttgg ccgacacgcc
     2341 gccggcgata caaagatcgg tgacccccgt ctcccgggcc gcccgccgaa gtttgttcag
     2401 gaggatggag acgatgcggc cctgaatgga ggcgcagata tcgggaagat tggcgctgac
     2461 aaaaccgggg tccttcgact cctggtccgc caggaagtag aggatggccg tcttgaggcc
     2521 gctgaagctg aagttgagtc ccgggatctg tggctcggga aaggagaagc gcgtagcgtc
     2581 gccggtgcgg gcatatttat cgatcagcgg gccgccgggg taggggaggc ccagcagttt
     2641 ggcggacttg tcaaaggctt cgcccgccgc atcatcgagg gtctcaccga tcacttccat
     2701 tttcaacggg gaatgacaaa gaacgatctg cgtatgtccc ccgctgacgg tgagacagag
     2761 aaaggggaag gagggtgtgc cggcatccgc ggagtccgtc gtcccggatg gacccgtcgc
     2821 cgcaccggcc ggcgcgataa gattggccat tacatgcgcc tgcatatggt gcaccgaaac
     2881 aaggggtata tccagcgcct gcgccagcga tttggcgaac tgcgaaccca ccagcagcga
     2941 cccgataagc cctggtgact gggtaaaggc gatggcattc agttcggata aggagacccc
     3001 ggccttctta agggctgcgt ccaccaccgg cacgatattc tgcatatgcg cccggctggc
     3061 caattcgggg acgaccccgc catattgctc gtggacggcc tgaccggcga tgatattggc
     3121 caggatggcg ccatcccggc tgatggcgca gctcgtttca tcacaggaag actcgatagc
     3181 taaaagggtc accggcccgg cattgggtgc ttgcatgccg caaaagtagg gaaatcgggc
     3241 gacgaccctt cgggccgtct gtcatttact gcggatcgcc acgacaggat ccattcgcgc
     3301 cgcgacaaag gcagggatga tccccgcggc caccccggtg aaaagacaga tggaaacggc
     3361 cagcaggatg atgttgagcg ggatgatcac ctggaagcgc aggatcgccg agaagatcag
     3421 cgtcaggata aagacggcca ccaggccgat gagcccgccg atgatgcaaa gaaaagcaga
     3481 ctcgagcagg aactccgtca ggatgacccc gcgcttggcg ccgatggctt ttttcagccc
     3541 gatctggctc gtcctttccc gcaccgtcac gaacatgata ttggctacgc caaacatccc
     3601 cacgatcagc gacagggccg cgatcgccca gccgccgata ttcatccccc ggaagatggg
     3661 gtcgaagaat ttggtgagcg tatcgatatt attcagggcg aaattatctt cctgggtagg
     3721 gctgagcttc cggatcgatc gcatggcgcc ccggagctcg tccgacaggg cctccgtgct
     3781 gatattggcg ccggcctggg ccagcagctt tggctgcgca ttctcttccc ggatcatctg
     3841 tttcatgaac gcataggtca ggaggataca attgtcgtag tcccatccgc cgataaggct
     3901 ctgcccctgt ttttcgatga cgccgatgac gatggcattc ctccctccct ttagcttgac
     3961 ggtctggccg atcgcctttt ccggcttgtt gaa
//
LOCUS       DATGFG010000683         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_136105, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000683 DATGFG010000000
VERSION     DATGFG010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136105"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VKQ52_12720"
     CDS             complement(<1..982)
                     /locus_tag="VKQ52_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HLZ88107.1"
                     /translation="MQMLQRLDYIVFLFYFVIVAVYGFWIYNRKKAAQTSSKDYFLAE
                     GALTWWAIGASLIASNISAEQMIGMSGSGFRLGLAISTYEWMAAATLLIVAIFFIPVY
                     LKNHIYTMPQFLSRRYNDTVAMIMAIFWLALYIVVNLMSILYLGALAVSSISGINIYV
                     CIVALAVFSIIITLGGMKVIGYTDVIQVFFLILGGLVATYIALHLVAEKSGQSGLLAG
                     FKIMTTEASDHFHMIFKKDSPNYVSLPGLSVLIGGMWIVNLNYWGCNQYITQRALGAD
                     LKTARSGILYAAFLKLLMPVIVVLPGIGAYVLYQKGMFHAGMLSSAGAVDV"
     gene            complement(1067..2395)
                     /gene="xylA"
                     /locus_tag="VKQ52_12725"
     CDS             complement(1067..2395)
                     /gene="xylA"
                     /locus_tag="VKQ52_12725"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF003998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HLZ88108.1"
                     /translation="MSVITGQKEFFKGIGPIKYEGPGSNNPLAYRWYDADKVVAGKTL
                     REHLRFAVAYWHSFVGNGADPFGEPTHIYPWNEKSDAVGRAKDKMDAAFEFITKLGLP
                     YYCFHDVDVVDYTNDVADNEKRLHTLTDYAKEKQAASGVKLLWGTANLFSHRRYMNGA
                     ATNPDFHVLAYAGAQVKAALDATIALGGENYVFWGGREGYMSLLNTNMKREQEHLARF
                     LHTAKDYARKQGFKGTFFIEPKPCEPTKHQYDYDSATVIGFLRQYDLLKDFKLNIEVN
                     HATLAGHTFTHELQVAVDAGMLGSMDANRGDYQNGWDTDQFPNNINELVEAMLIILEG
                     GGFQGGGINFDAKRRRNSTDAADLFHAHIGGIDCFARALLTANAILQGSDYWKVRAER
                     YASFDGGEGAAFEKGKLSLEELRNYAVANGEPKVVSGRQEYLENLINRYI"
     gene            complement(2448..>2680)
                     /locus_tag="VKQ52_12730"
     CDS             complement(2448..>2680)
                     /locus_tag="VKQ52_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88109.1"
                     /translation="FKSNVFIDAFVNATNVPVELYNSDGSIGAAIGAGIGAGIYRDAK
                     EAFAGLKPVELIEPTRAGVYDALYGEWKQHLP"
BASE COUNT          658 a          859 c          604 g          559 t
ORIGIN      
        1 tcacatccac cgcacccgcc gagctcagca tcccggcatg gaacatgccc ttctgataca
       61 ggacataggc gccgattccc ggcaacacca cgatcaccgg catcagcagc ttcagaaagg
      121 cggcataaag gataccgcta cgcgccgttt tcaggtccgc gccaagcgcc cgctgcgtga
      181 tatactggtt acacccccag tagttcaggt tgacgatcca catccccccg ataagcaccg
      241 acagtccggg cagactcaca taattgggcg agtctttctt gaagatcata tggaaatggt
      301 cactggcctc cgtcgtcatg atcttaaacc cggccagtag cccgctctga cccgacttct
      361 ccgccaccag gtgcaacgca atataggtag ccaccagccc gcccaggatc aggaagaaca
      421 cctggatcac atccgtatac ccgatcacct tcatcccccc cagcgtaata ataatggaaa
      481 acaccgccaa tgccacgata cagacataga tattgatccc cgagatactg ctcaccgcca
      541 gcgcccccag gtagaggatc gacatcaggt tgaccacgat atacaacgcc agccagaaaa
      601 tggccatgat catcgccacg gtgtcattgt accggcggct gaggaactgc ggcatcgtat
      661 aaatatgatt cttcaggtat accgggataa aaaagatggc cacgatcagc agggtcgcag
      721 ccgccatcca ctcatacgtg gagatggcca gccccagccg gaaacccgag ccgctcatcc
      781 cgatcatctg ctcggccgag atattcgacg cgataagcga agccccgatg gcccaccagg
      841 tcagtgcccc ctctgccagg aaataatcct tggaactggt ctgcgccgcc ttcttccggt
      901 tatagatcca aaaaccataa acggcaacga tcacgaagta aaaaaggaaa acaatgtaat
      961 ccagcctttg taacatctgc atatggcaaa aaaattaggg tgagcccgac aggtatacgc
     1021 cagggcaaaa aataaccccc ggtaccacac cgggggctac cagaggctaa atatagcgat
     1081 ttataagatt ttccaaatat tcctgccgcc cgctgacgac tttcggttcc ccattggcga
     1141 cggcatagtt acgcagctct tccaggctca gcttcccttt ctcgaatgcc gccccttccc
     1201 caccatcgaa cgacgcatag cgctcagcgc gcaccttcca gtagtccgaa ccctgcagaa
     1261 tggcgttcgc ggtcagtaac gcccgggcaa aacaatcgat cccaccgata tgcgcatgga
     1321 aaaggtccgc cgcatccgtg gaattccgcc ggcgcttggc gtcgaaattg atcccgccac
     1381 cctggaaacc acccccttcg aggatgatca gcatggcctc caccagttcg ttgatattat
     1441 tcgggaactg atcggtgtcc catccattct ggtaatcgcc acggttggca tccatggagc
     1501 caagcatacc cgcatccacg gctacctgca gttcatgggt aaacgtatgc cccgccaggg
     1561 tcgcatgatt cacctcgata ttcagcttga aatccttcag caggtcatac tgccgcaaaa
     1621 aaccgatcac cgtcgcactg tcataatcat actggtgctt ggtcggctcg cagggcttcg
     1681 gctcgataaa gaacgtaccc ttgaaaccct gcttccgggc atagtccttc gccgtatgga
     1741 gaaaacgcgc caggtgttcc tgctcccgtt tcatgttcgt attgagaaga ctcatatagc
     1801 cttcccgccc gccccagaag acatagttct cgccgccaag cgcgatcgtg gcgtccagcg
     1861 ccgccttcac ctgcgcaccc gcataggcca gcacatgaaa atcaggattg gtagccgcac
     1921 cgttcatata ccggcgatgc gagaaaagat tggcggtgcc ccacaaaagc ttcaccccgc
     1981 tggccgcctg cttctccttc gcatagtcgg tcagggtatg cagccgcttc tcgttgtcgg
     2041 cgacatcatt cgtataatcc acgacatcca catcgtgaaa acaataataa ggcaatccca
     2101 gtttcgtgat aaattcaaaa gccgcatcca tcttgtcctt cgcccggccc accgcatcgc
     2161 tcttctcatt ccagggatag atatgcgtcg gctcgccaaa aggatcggcg ccattgccca
     2221 caaaagaatg ccaataggca accgcaaatc gcaaatgctc tctcagcgtc ttcccggcca
     2281 cgaccttgtc cgcatcatac caccggtagg caagtggatt attcgaaccg ggtccctcat
     2341 acttgatggg gcctattcct ttaaagaact ccttctgtcc cgtgataact gacataactt
     2401 gatagtttat taattaatgt accggagccg ctttccagcg ccccctgtta aggtagatgt
     2461 tgcttccatt ctccatacaa cgcatcatag acccccgccc gcgtcggctc gatcagctcc
     2521 accggcttca accccgcaaa ggcttccttc gcatcccggt agatccccgc tccgatgcct
     2581 gcgccaatcg ccgccccgat acttccatcg ctgttgtata attccaccgg cacgttcgtg
     2641 gcatttacga acgcatcgat aaaaacgttg cttttgaaca
//
LOCUS       DATGFG010000684         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1257671, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000684 DATGFG010000000
VERSION     DATGFG010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257671"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VKQ52_12735"
     CDS             complement(<1..223)
                     /locus_tag="VKQ52_12735"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HLZ88110.1"
                     /translation="MSTKNLTLIVVLVVILLFGGCACSRYNGLVTTDQGVKTSWSNVE
                     AQYQRRTDLYNSIIKTIEGSANFEKSTLKE"
     gene            complement(307..1488)
                     /locus_tag="VKQ52_12740"
     CDS             complement(307..1488)
                     /locus_tag="VKQ52_12740"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HLZ88111.1"
                     /translation="MINYTLRKRLRKLRILYCAGLGLLLPALLHAQPLPIKVENNPAA
                     RTITITAGGKPFTTFIYPDSLAKPVLYPIYAADGQLITRGFPLATRAGEPVDHPHHLG
                     LWFNYENVNGFDFWNNSYAIPAAKKAQYGWIRTDSILEAGNAGPKSGDQHYPNTNTTD
                     PASTGRAMPGHAAGMNVANGLGIIRYAARWTNQQNETLLTERTTYIFSAGKDERIITR
                     LTTLTAVADGGVSFPDAKDGMLGLRVTKELQIPSNTPGEFVDDKGNITKVAAGNTPDI
                     NGNYLTSEGKAGDSAWGTRGNWCLLYGKKQQDTLSIAIIDHPANPGYPTYWHARGYGL
                     FAANPLGEKIFSNGRQTMNFRLEKGQSVTFRYRIVITAGNNRLSTGRIRQLADDFARV
                     K"
     gene            1550..2908
                     /locus_tag="VKQ52_12745"
     CDS             1550..2908
                     /locus_tag="VKQ52_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ88112.1"
                     /translation="MTTTRRDFIRKSSMAGMATYMGALGLSASSYRRIIGANDRVRVG
                     VVGFSDRHRNDHIPAFLKSYQEMNFDIIAVSDIWKKRREEGSAFLAQKFGHPVQACVN
                     NDALYNIKEVDAVMISTADFQHAWHTVEAVKAGRDTYTEKPLAETMEDARAVLKAVKD
                     SGRIVQIGSQRRSGANYWAAADYIQSGKFGPIVMVELTWNVNQPGRWRRPALVSQLKE
                     EDTDWKRFLINRPYEPFDARKHLEFRLFWPYSSGLPGQWMSHQIDTVHWFSQLPHPRS
                     ATANGGIYAWKDGRRNADTMTVVFDYGPLTDASTGFQVVFTSRQTNSAGETKEIYYSN
                     GGTMNLDTNKISHEGGLTERYASAMGMHANELPEISLKELEFKASTEASTGGDPLTTA
                     HVRNWMECVRSRKQPNAPIDAGYHHSIAVIMANAAYRTGERVTFDEKTQEVMAGGKVF
                     KY"
     gene            2916..3965
                     /locus_tag="VKQ52_12750"
     CDS             2916..3965
                     /locus_tag="VKQ52_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLZ88113.1"
                     /translation="MKALVITEKNKPVELLSVADRVVGPGQALVRVYAAALNHRDVWI
                     QKGQYAGLKYPIVPGSDGAGVVVAVSDGGSGGGTAGVRGGSGVAAARDWIGKEVIIDP
                     ALHWGEAAGFQSPTNFKILGLPDDGTLAEYVVAPLTNLVAKPAHLSFEEAAALPLAGV
                     TAFRASMTRAQLRAGDRVLITGIGGGVALFALQFAVAAGAKVYVTSGDDEKLSRAMSM
                     GAAGGANYKHSGWVESLRSQAGAYDVIVDGAGGDGVNELFDLATPGGRVVFYGATCGN
                     PSSVVLRRIFWKQLNVLGSTMGSPADFAAMVEFVGRHGIRPVVDRVFAMEEGEAAFRH
                     MDDAKQCGKIVIKIK"
     gene            complement(4030..>4300)
                     /gene="pgk"
                     /locus_tag="VKQ52_12755"
     CDS             complement(4030..>4300)
                     /gene="pgk"
                     /locus_tag="VKQ52_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLZ88114.1"
                     /translation="IHRSKTILWNGPMGVFEMTKFQRGTKGIARAVAEATEKGAFSLV
                     GGGDSVAAVNQFGYADKVSYVSTGGGAMLEYFEGKELPGIAAIKG"
BASE COUNT          806 a          955 c         1540 g          999 t
ORIGIN      
        1 cttccttgag ggttgatttt tcgaaattgg cggagccttc gatagtctta atgatactgt
       61 tatatagatc ggtacgtcgt tgatattggg cttcgacatt gctccaggat gtttttacgc
      121 cctggtcggt agttaccagg ccgttgtagc ggctgcaggc gcaaccgccg aacaggagga
      181 tgacaactaa tactacgatc agggttagat tcttcgtact cattttttac atttttaggt
      241 taacgcggta aaaatagcac ttttggcgag gcccggctac cttttttagg ccggtggggc
      301 gctttgtcat tttaccctgg caaaatcgtc ggcgagctgc ctgatcctgc cggtggagag
      361 gcggttgttc ccggcggtga tgacgatgcg ataccggaaa gtaaccgact ggcctttctc
      421 caggcgaaag ttcatggtct gccggccatt gctgaagatc ttttctccca gcgggttggc
      481 ggcaaaaagg ccatagccgc gggcatgcca ataggtgggg taaccggggt tagcggggtg
      541 gtcgatgata gcgatgctaa gggtatcctg ttgttttttg ccgtagagca ggcaccagtt
      601 acctcttgtt ccccaggcgc tgtcgcctgc tttcccttcg ctggtgagat agttgccgtt
      661 gatgtcgggg gtgttgccgg cggcgacctt cgtgatattg cctttatcat cgacgaattc
      721 gcccggggtg ttggagggga tctggagttc ctttgtgacg cggaggccga gcatgccgtc
      781 ctttgcgtct gggaagctga ctcctccgtc ggcgacggcg gtgagggtgg tgaggcgggt
      841 gataattctt tcatccttcc cggcgctgaa gatataggtg gttcgctccg tcagcagggt
      901 ttcgttttgc tggttcgtcc agcgggcggc gtagcggatg atgcccagtc cgtttgcaac
      961 gttcatgccg gcggcgtgac cgggcatggc gcgaccggtg gaagccggat cggtcgtatt
     1021 cgtattggga tagtgctgat cgccgctttt tggtccggcg ttgcctgctt cgaggatgct
     1081 gtcggtgcgg atccagccgt attgtgcttt tttagcggcg gggatggcgt aggagttgtt
     1141 ccagaagtcg aagccgttga cattctcata gttgaaccag aggccgagat gatgcgggtg
     1201 gtcgacgggt tcgccggcgc gggtggcgag ggggaagccg cgggtgatga gctggccgtc
     1261 ggcggcatag atgggataga gcacgggttt tgcaaggcta tcggggtaga tgaaggtcgt
     1321 gaagggtttt ccgccggcgg tgatggtgat ggtgcgggcg gcggggttgt tttccacctt
     1381 tatggggagg ggttgggcgt ggaggagggc tggaaggagg aggccgaggc cagcacagta
     1441 caggatgcga agtttacgaa gacgtttgcg gagagtgtag ttgatcatat aacaggcgtt
     1501 tgcttgagca atgatgtaaa ttagtatttt taactaaaca ttcttccata tgacaacgac
     1561 ccgcagggat tttatccgca agagcagcat ggccgggatg gccacgtata tgggcgcgct
     1621 tgggctcagc gccagcagct atcgccggat catcggcgcc aatgatcgtg tacgcgtggg
     1681 ggtggtaggc ttttccgatc ggcaccgaaa tgaccatatt ccggctttcc tgaagagtta
     1741 ccaggagatg aatttcgata ttatcgcggt gtcggatatc tggaagaagc gccgcgagga
     1801 agggagcgcg ttcctggcgc agaagttcgg gcatccggtg caggcctgtg tgaacaacga
     1861 cgcattgtac aatataaaag aggtggatgc cgtgatgatc tcgacggcgg atttccagca
     1921 tgcctggcat acggtggaag ccgtgaaggc cggcagggat acctatacgg agaagccgct
     1981 ggccgagacg atggaagatg cgcgggcggt gttgaaggcg gtgaaggatt cgggcaggat
     2041 cgtgcagatc gggtcgcagc ggcggagtgg cgctaattat tgggcggcgg cggattatat
     2101 ccagtcgggg aaattcgggc cgatcgtgat ggtggagctg acatggaatg tgaatcagcc
     2161 gggtcgctgg cggcggccgg cgctggtgtc gcagttgaag gaggaggata cggactggaa
     2221 gcgattcctg atcaaccggc cctatgagcc gttcgatgcg cggaagcatc tcgagttccg
     2281 cttgttctgg ccgtattcgt ccgggctgcc ggggcagtgg atgagtcatc agatagatac
     2341 ggttcactgg ttctcccagt tgccgcatcc gcgaagcgcg acggctaacg ggggtatcta
     2401 tgcgtggaag gatggccgga ggaatgcgga tacgatgacg gtggtgttcg attatggtcc
     2461 tttaaccgat gcgtccacgg gcttccaggt ggtgtttact tcgcggcaga cgaattcggc
     2521 gggtgagacg aaagaaattt attattccaa tggcggaacg atgaacctgg atacgaataa
     2581 gatctctcat gaaggcgggt tgacggagcg atatgcgagt gcaatgggga tgcatgcgaa
     2641 tgagttgccg gagatctcgc tgaaggaact ggaattcaag gcgtcgaccg aggcgagtac
     2701 cggaggcgac ccgctgacga ctgcgcatgt gcggaactgg atggagtgtg tacggagccg
     2761 gaagcagccg aatgcaccga tcgatgcggg gtatcatcac tcgattgcag tgatcatggc
     2821 gaatgcggcg taccggaccg gggagcgggt gacgttcgac gagaagaccc aggaggtgat
     2881 ggcggggggg aaagttttta aatattaaaa cgactatgaa agcattggtt ataacggaga
     2941 agaataagcc ggtggaattg ttatcggtgg cggacagggt ggttggcccg gggcaggcgt
     3001 tggtgcgggt gtatgcggcg gccttgaatc accgggatgt gtggatacag aaggggcagt
     3061 atgcggggtt gaagtatccg atcgtgccgg gttcggatgg cgcgggcgtg gttgttgcgg
     3121 tgagtgacgg tggttcaggc ggcgggacgg cgggagttcg cgggggttcg ggcgttgcgg
     3181 cggcgcgtga ttggatcggg aaggaggtga tcatcgatcc ggcgttgcat tggggagagg
     3241 cggctggttt tcagagcccg accaatttca agatattggg cctgcccgat gacgggacgc
     3301 tggccgagta tgtggtggcg ccattgacca acctggtcgc caagccggcg catctttcct
     3361 ttgaagaagc ggcggccctg ccgttggcgg gagtgacggc gttccgcgcg tcgatgacgc
     3421 gggcgcagtt gcgggccggg gaccgggtgt tgatcacggg catcggcggc ggtgttgccc
     3481 tgtttgccct gcagtttgcg gtggcggcgg gggcaaaggt gtatgttacc tcgggagacg
     3541 atgagaagct gtcgcgggcg atgtccatgg gcgcggccgg cggagccaat tataaacatt
     3601 ccggctgggt ggaatcgttg cgctcgcagg ccggggctta tgatgtgatc gttgacggag
     3661 ccggtggaga tggggtgaat gagctgttcg acctcgctac gccgggtggc cgggtagttt
     3721 tttatggcgc gacatgcggg aacccgtcgt ccgtcgtgtt gcggcggatc ttttggaagc
     3781 agctaaatgt tttggggtcg acgatgggga gtccggcgga ttttgcggcg atggtagaat
     3841 ttgtgggccg tcatgggatc cgcccggtgg tggaccgggt gtttgcgatg gaggagggcg
     3901 aggcggcgtt ccgccatatg gatgatgcga agcagtgtgg gaagatcgtg atcaaaataa
     3961 aatgaggggc ttgcgcccct ctttctatgc ttttatcgca gcgatgcgga tcgaggctcc
     4021 gttcgtattt tatcccttga tggcagcgat gccggggagt tcttttcctt cgaagtattc
     4081 gagcatggct ccgccaccgg tggacacata gctcacctta tcggcgtagc cgaactggtt
     4141 gacggcggca acggagtctc caccgcctac gagcgagaag gcgccttttt cggtagcctc
     4201 ggccacggcc cgggcgatgc cctttgtccc gcgttggaat tttgtcattt caaatactcc
     4261 catgggaccg ttccagagga tggtcttcga tcgatggatg
//
LOCUS       DATGFG010000685         5479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_478873, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000685 DATGFG010000000
VERSION     DATGFG010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478873"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..394
                     /gene="rnpB"
                     /locus_tag="VKQ52_12760"
     ncRNA           2..394
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VKQ52_12760"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(439..1374)
                     /locus_tag="VKQ52_12765"
     CDS             complement(439..1374)
                     /locus_tag="VKQ52_12765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HLZ88115.1"
                     /translation="MRAFLNYLTAYLRSSSRPALLITILFIAALVAANYTIGIENRIR
                     AFTPWYLAIGGFFLFYAIVLALVWGLQSEWNHTPIGQPKLLLLLGLAPLYFALKMVHW
                     DLLPLLPHGITAPWDHYTQLVLQLPLKLLLLFIFLWILRKADLLPGDSLTDAVGLTRG
                     NFNTLPYFGLLAMMLPLIALASTQHDFLQVYPKVKNLAFIDGYAKPEWPWKLLYEISY
                     GLDFLGIELFFRGLLVVALFNYVGQAAILPMAAFYCTIHFGKPLGECISSFFGGLILG
                     VLAARTRTILGGLIVHLGLAWLMEFGGWLGSLWQH"
     gene            complement(1374..2321)
                     /gene="plsX"
                     /locus_tag="VKQ52_12770"
     CDS             complement(1374..2321)
                     /gene="plsX"
                     /locus_tag="VKQ52_12770"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HLZ88116.1"
                     /translation="MSMNIGLDMMGGDFSPLEAVKGVQLYLKDAKAPATVWLVGDENQ
                     VRPLLQEYAIPEGSVRVVHAPQVIGMNEHPTKALKEKQQSSIAIGFHLLATGKIDAFI
                     SAGNTGAMLVGALYSLKPIEGIQRPTISTVIPKENGKTGVLLDVGLNADCKAENLNQF
                     AVLGSLYAQHILGIENPRVGLLNIGEEEGKGNILAQATYPLLKENSQINFIGNIEGRD
                     ILLDKADVTVCEGFTGNVILKLAESLFDITSRHKTESEYFDRFDFEYYGGTPLLGVTK
                     PVIIGHGISRSKSFLNMIKLAQKMLDTDLLGKMKEYFLS"
     gene            complement(2334..2525)
                     /gene="rpmF"
                     /locus_tag="VKQ52_12775"
     CDS             complement(2334..2525)
                     /gene="rpmF"
                     /locus_tag="VKQ52_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HLZ88117.1"
                     /translation="MPNPKRRHSQQRSAKRRTHYKAEKVTLSKDSTTGEIHVRHRAHV
                     SEGKLFYKGKVVVETSPVK"
     gene            complement(2567..3121)
                     /locus_tag="VKQ52_12780"
     CDS             complement(2567..3121)
                     /locus_tag="VKQ52_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HLZ88118.1"
                     /translation="MSSRREFEIAFVGLKAGIHEFSYRITDKFFEAYQQQDFHRCDAQ
                     VKLTLDKKNSFMLLKFEITGKVEIACDRCGNQLPLDLWDEFNILVKLVEDPEIMNQQE
                     EDPDVYYISKGESHLHVSDWIYEFINLSIPMQRMCKPEEMGGPHCNKEVLEMLKKLDR
                     EKSQPGNPLWKGLEKFKDLPPGNN"
     gene            3302..4546
                     /locus_tag="VKQ52_12785"
     CDS             3302..4546
                     /locus_tag="VKQ52_12785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ88119.1"
                     /translation="MRLFTKYNRVNIVASILALLLGSISYYFAVRYVLVSELDDNLRT
                     EEAEVLDHVRTRNELPEPASYKDQQVRFEPAARQVKRQFQHTRLRMGRHHWEPFRELV
                     FSVEIAGQWHTVFIDVSEEETEDLLMLIMLITAGMIILLLSLLFIANRLLLRRIWQPF
                     YQTLNGIRKFNLSSPEPLPVQNSDIDEFRSLDEAIRQMTQTITKDYEMLKNFADNASH
                     EMQTPVAIINSKLDLLIQDTALSMEHHRPIQAMYDAVGRLRQLNQSLLLLTKIENNQF
                     VSETVDLAPLIERKLVQLEDPVKDRRLTVHTELDALQLPINGYLADILLNNLLTNAIR
                     HNQDGGQVNIRLRGRGLRVSNSGAALGFDASTIFDRFTKGMHSGGTGLGLAIVRQICD
                     NYHFGLTYSYAGEMHTIDITFN"
     gene            4549..5175
                     /locus_tag="VKQ52_12790"
     CDS             4549..5175
                     /locus_tag="VKQ52_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88120.1"
                     /translation="MMRNLYVLILCLGSSCLHAQYFVGKVIYRNVVTSKMPGLSDSAL
                     TVLIGSEESYFIKGGFYQSLTNGAGFSMQQYDHRTNRLYFKKPDVDTLYWVDAGKSSD
                     KPVSFETREDAEVVLGKRCDALVVQTANGTTVFYYSPRYKLDAAMFMKHSYGGWAFYV
                     GQTGCLPLKTVIDNDRFHLESTATEILELDLKDSFFEISPKLPQVKSR"
     gene            5248..>5479
                     /locus_tag="VKQ52_12795"
     CDS             5248..>5479
                     /locus_tag="VKQ52_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88121.1"
                     /translation="MLRILIVGLALICGLVVEGQPVTRLDFYVDQALQNSPLLKDYQN
                     QLQAGQVDSQLIRAGYRPQVTGSSVNLWAPTVN"
BASE COUNT         1178 a         1313 c         1718 g         1270 t
ORIGIN      
        1 cgctaccaac cgggtcatcg ctctccgttc tgcgggggga ggaaagtccg gacagcgcag
       61 ggcaatgcac cggttaagag ccggggggcg cgcaggcgtt cacagagagt gccacagaaa
      121 ataactgccc cgcggatcgc ggggtgagga tgaaaacgtg gggtaagagc ccacggcgcc
      181 ggcgggtaac cgccggcgcg ggtaaacctt gcatgctgaa atgccacgta taccggcgac
      241 cgagggcggc tcgcccgatg cttccgcgaa ggggcttcgc cggagggtag gcagctagat
      301 ccctgcagta atgcaggggc cagataaatg atggccgccg cgtacgacgc gagtctgcgc
      361 ggaacaggat ccggcttaca ggttggtagc ttttttttaa tttatcctat gctctccggc
      421 gggatggggc gtttcgggct agtgttgcca tagggagccg agccagccgc cgaactccat
      481 gagccaggcg aggccgaggt ggacgatgag cccgccgagg atggtgcggg tgcgggcggc
      541 gaggacgccg aggatcaggc caccgaagaa ggaggagatg cattccccga ggggtttacc
      601 aaagtggatc gtgcaataga aggcggccat gggaaggatg gctgcctggc cgacatagtt
      661 gaagagggcg acgacgagaa gcccgcggaa gaagagttcg atgccgagga agtcaagacc
      721 gtaagagatc tcgtacaaca gtttccacgg ccattccggt tttgcgtagc catcgataaa
      781 ggccaggttc tttacttttg ggtagacctg gaggaaatcg tgctgggtcg aggccagtgc
      841 gatgagggga agcatcattg ccaacaaacc aaagtagggg agggtattga aattaccgcg
      901 ggtgaggccg acggcgtcgg tgagactatc gcccggcagg agatcggctt tgcggaggat
      961 ccagaggaag ataaacagca gcaggagctt taatggcagc tggaggacga gctgggtata
     1021 gtgatcccag ggagcggtga tgccgtgcgg caggagggga aggagatccc agtggaccat
     1081 tttcagcgcg aaatagaggg gggcgaggcc gaggaggagg aggagttttg gctggccgat
     1141 gggggtatgg ttccactcgg attggaggcc ccagacgagg gcgaggacga tggcgtagaa
     1201 aagaaagaag ccgccgatgg cgagatacca gggggtaaag gcccggatgc ggttctcgat
     1261 gccgatggtg taattggcgg cgacgagggc ggcgataaag aggatggtga tgaggagggc
     1321 agggcgggag ctgctgcgca gataagccgt gagatagttg aggaaggcgc gcatcaggaa
     1381 agaaaatatt ctttcatttt gcccagcagg tcggtgtcga gcatcttttg cgccagcttt
     1441 atcatgttga ggaaggactt gctgcgggag atgccgtgac cgatgatgac gggttttgtc
     1501 acgccgagca ggggggtgcc gccgtagtat tcgaagtcga agcgatcgaa gtattcgctt
     1561 tccgttttat gccggctggt gatgtcgaag agggattcgg ccagtttgag gatgacgttg
     1621 ccggtgaaac cttcgcagac ggtgacatca gctttatcga gcaggatgtc gcggccttcg
     1681 atattgccga tgaagttgat ttggctgttc tcttttaaca ggggataggt agcctgggcg
     1741 aggatattgc ccttgccttc ctcttcgccg atgttgagta acccgacgcg ggggttttcg
     1801 atgcccagga tatgctgggc atagagtgaa ccgaggaccg cgaactggtt gagattttcc
     1861 gctttgcaat cggcgttgag gccgacgtcc agcagaaccc ccgttttgcc gttctctttt
     1921 ggaatgacgg tcgaaatggt ggggcgttgg atgccttcga tgggtttcag gctgtaaagg
     1981 gcgccgacga gcatggcccc tgtgttaccc gcgctgataa aggcatcaat tttcccggtg
     2041 gccaggaggt ggaagccgat ggcgatggag gactgttgtt tttccttcaa cgcctttgtc
     2101 ggatgttcgt tcatcccaat tacctggggc gcatggacga cccggaccga cccttcggga
     2161 atggcgtatt cctggagcaa gggccggacc tggttttcgt caccgaccaa ccatacggtg
     2221 gcgggagctt ttgcatcctt caggtagagc tggacacctt tcaccgcttc aaggggtgaa
     2281 aagtctcctc ccatcatgtc gagaccaatg ttcatgctca ttggcgaagc ggcttactta
     2341 acgggagaag tttctaccac tactttaccc ttgtagaaca gcttgccttc gctgacgtgg
     2401 gcacgatgac gaacgtggat ctcgccggtg gtgctgtctt tgctcagggt cactttttca
     2461 gctttataat gcgtccttct cttggcactg cgctgctggg aatgtctgcg tttcggattt
     2521 ggcattttct gtttatttta caataaccta tacaaaatat tgattcttag ttgtttcccg
     2581 gcggcaaatc cttgaatttc tcaaggcctt tccacagggg gttaccgggt tggctctttt
     2641 cccggtcgag ttttttcagc atttcgagca cttccttatt gcagtgcggc ccgcccattt
     2701 cttcgggttt gcacatgcgc tgcatgggga tgctgaggtt gatgaactcg tagatccagt
     2761 cggatacgtg caggtggctt tcccccttgg atatataata tacgtcgggg tcttcttcct
     2821 gctggttcat gatctcaggg tcttccacca gtttgaccag gatgttgaat tcgtcccaga
     2881 ggtcgagcgg gagctggttg ccgcagcggt cgcatgcgat ctcgaccttc ccggtgatct
     2941 cgaacttcag gagcatgaag ctgttctttt tatcgagtgt cagcttcacc tgggcatcgc
     3001 aacggtggaa gtcctgttgc tgataagcct caaagaactt atcggtaatc cgatagctaa
     3061 actcatgaat accagcctta aggcccacaa acgctatttc aaattcccgg cggctgctca
     3121 tgagtgcttt ttaaagagct tgcaaaggta gtgataaatt atcagaaaaa atggggtttg
     3181 gagatctttt tttaaccttc aactccctca ggccgaacgg agtgcggcct ggtcattatg
     3241 atcgttgcgg caaaaaatta tattttgacc attttttgcc gcaatcctta aattacaacg
     3301 aatgaggctt tttaccaaat acaaccgggt caacatcgtt gccagcatcc tggcgttgtt
     3361 gctgggtagc atcagttatt atttcgccgt tcgttatgtt cttgtctccg aactggatga
     3421 taatctgcgc accgaggaag ccgaggtgtt ggatcatgtc cggacgcgga acgagttgcc
     3481 ggagccggcg agctacaagg atcagcaggt tcgcttcgag ccggcggccc ggcaggtgaa
     3541 gcggcagttc cagcatacgc ggctgcggat ggggcgccat cattgggagc ctttccggga
     3601 gctggtgttc tcggtagaga tagcggggca gtggcatacg gtatttatcg atgtgtcgga
     3661 ggaggagacg gaggatctgc tgatgctgat catgctgatc acggcgggga tgatcatttt
     3721 gttgctgagc cttcttttta ttgccaaccg gctcctgctt cggcggatct ggcagccttt
     3781 ttaccagacg ctgaacggga tccggaaatt caatttatcg agtcccgagc ccttgccggt
     3841 gcagaacagc gatatcgacg agttccgcag cctggatgag gcgatccggc agatgacgca
     3901 aaccattacc aaggactatg agatgctgaa gaatttcgcc gacaatgcct cgcatgagat
     3961 gcagaccccg gtggcgatca tcaattccaa attggatctg ctgatccagg acacggcgtt
     4021 gagcatggag catcaccggc cgatacaggc catgtatgac gccgtcgggc ggctgcggca
     4081 gctgaaccag tcgctgttgc tgctgacgaa gatcgagaac aaccagttcg tttccgagac
     4141 ggtggacctg gcgccgctga tcgaacggaa gctggtccag ctggaagacc ccgtaaagga
     4201 ccggcggctc acggtgcata ccgagctcga tgcattgcaa ttgccgatca acggttatct
     4261 tgcggatatt ctgttgaaca acctgctcac caatgcgatc cggcataacc aggatggggg
     4321 acaggtgaat atccggctcc gcgggcgcgg gctccgggtg agtaactccg gtgcggcgct
     4381 tggcttcgat gcctcgacga tcttcgaccg gtttaccaag ggcatgcact ccggcgggac
     4441 ggggcttggg ctggcgatcg tgcggcagat ttgtgacaat tatcattttg gcctgaccta
     4501 ttcgtatgcg ggagagatgc atacgatcga tataaccttc aactgacgat gatgcgcaac
     4561 ctttacgtac tgatcctttg tcttgggagt tcctgtctgc acgcccagta ttttgtcggg
     4621 aaggtcattt accggaacgt ggttacaagc aagatgcccg gcctttccga ttctgcgctc
     4681 accgtcctga tcgggagtga ggagagttat tttatcaagg ggggatttta ccagtccctc
     4741 accaatggcg ccgggttttc gatgcagcaa tacgatcacc ggacaaaccg gttatatttc
     4801 aagaaaccgg atgtcgacac cctgtactgg gtggatgccg gcaagagctc ggataagccc
     4861 gtttcgtttg agacgaggga ggatgcggag gtggtgctgg ggaaacgctg cgatgcgctt
     4921 gtcgtgcaga cggccaatgg aacaacggta ttctattaca gtccccggta caagttggat
     4981 gcggcgatgt tcatgaagca cagttatggg ggctgggcct tttatgtcgg ccagaccgga
     5041 tgtctgccgt tgaagaccgt tatcgataac gaccggttcc atctcgagag cacggcgacg
     5101 gagatactgg aactggatct gaaggatagt tttttcgaga tctcaccgaa gcttccacag
     5161 gtgaagtcca ggtaattccc ctacagaatt tcaacaggat agcggaatac ttttggcggg
     5221 gccacacacg gggccttgaa atataatatg ttgcgtattt tgatcgtcgg attggcattg
     5281 atctgtggcc tggtggtgga gggacagccc gttaccaggc tggattttta tgtagaccag
     5341 gcccttcaga acagccccct gctgaaggat taccagaatc agctacaggc ggggcaggtg
     5401 gacagtcaac tgatccgggc gggttaccgg ccgcaggtga cggggtcgag tgtcaacctc
     5461 tgggcgccca ccgtcaacg
//
LOCUS       DATGFG010000686         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_372602, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000686 DATGFG010000000
VERSION     DATGFG010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372602"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VKQ52_12800"
     CDS             <1..232
                     /locus_tag="VKQ52_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88122.1"
                     /translation="TEGPLLAFLRRDNGSSVLVAVPLIRYETPFPSAVAITLPQDAPR
                     YWTDAFTGETLGPELSWPKGRTTWPVILATSR"
     gene            259..864
                     /locus_tag="VKQ52_12805"
     CDS             259..864
                     /locus_tag="VKQ52_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HLZ88123.1"
                     /translation="MSILVLVRHGQSTYNLENRFTGETDVPLTPRGREEARAAGKKLT
                     GIPFSHGFTSVLQRAVESMTLLLEEAHQQQLPVTRDRALNERNYGDLQGLNKAEIAKQ
                     YGNEQVAIWRRSFSVRPPGGESLADTYNRVVPYYQQHIASLLQKGENILIVAHGNSLR
                     ALMMYLEKISEEEIPHVDLPTGRPRQYTLDAKGAVTAAADL"
     gene            924..1541
                     /locus_tag="VKQ52_12810"
     CDS             924..1541
                     /locus_tag="VKQ52_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88124.1"
                     /translation="MQLPSLNRWHFIFAATAFFAYSMTGCSKNSNPNNNRNDSDTVLH
                     RTVYDSAKFYITFDMEIAKTSGVFNDTFSDHASMIVYVVNGVVKVPPDSLRNFPPIVY
                     PASGSSGAWSATWIPDNIGEINVTGATGLVTADTAVALILTQTGCVTPDWNISFMGGA
                     PSSTGNNPSPGWPLVLTFNPKLKSQYPFKLDQAGSSWAIWCYKDY"
     gene            complement(1568..>2473)
                     /gene="bshC"
                     /locus_tag="VKQ52_12815"
     CDS             complement(1568..>2473)
                     /gene="bshC"
                     /locus_tag="VKQ52_12815"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HLZ88125.1"
                     /translation="ADRPAFKRQMIAVFEDDLFRQESSRIVSYSIERLGEHYDIQANP
                     RDINLFYLKDELRGRIGQVGDVYKIHGSSFTFTADELRDELHRYPERFSPNVILRGLF
                     QETILPNLAFVGGGGETAYWLELKGLFDHYQTPFPVLVLRNSFLLVQRQWMEKMERAG
                     FGITDLFRDEQDLLNQLVRRDSTQTLSLEEEIATANHYYESLKALARPVDPTLEQHVA
                     ALQTKAVEPIRLLEKKLLRAEKRKFGDQQRQIHAVKEALFPHGSLQERVENILPWFAA
                     YGPAVIRDLYDQSPVWGEFVVLTEP"
BASE COUNT          544 a          794 c          628 g          507 t
ORIGIN      
        1 caccgaaggc cccctgctcg cctttctccg tcgcgacaac gggagctccg tccttgtcgc
       61 ggtgccgctt atccgctacg aaaccccctt ccccagcgcc gtggccatca cccttccaca
      121 ggatgcccct cgctactgga ccgatgcctt caccggcgaa acactcggcc ccgaactctc
      181 ctggccaaaa ggccgcacca cctggcctgt catcctggcc acctcccgat gatgtatctt
      241 tatcccaaat cacccgcaat gtccatcctg gtccttgtcc gccacggcca atccacctat
      301 aatctcgaga accgcttcac cggtgagacc gatgtcccgc tgacaccccg gggccgcgaa
      361 gaagcaaggg ccgccgggaa aaagttgacc ggcatcccgt tctcccatgg cttcacttcc
      421 gtcctgcaac gggccgtcga atcgatgacc ctcctgctcg aagaagccca ccagcaacag
      481 ctcccggtca cccgtgatcg cgcgctgaac gagcggaact acggggatct gcagggtctc
      541 aacaaggcgg agatcgcaaa acaatatggg aatgaacagg tggccatctg gaggagaagt
      601 ttctcggtgc gcccacccgg cggcgaaagc ctcgccgaca cctataaccg ggtggtcccc
      661 tattatcagc agcatatcgc gtccttgctg caaaaaggcg aaaatattct catcgtcgct
      721 catggcaaca gcctgcgcgc cctcatgatg tatctcgaaa agatcagtga agaagagatc
      781 ccgcacgtcg atctgcccac cggccggccc cgccaatata ccctcgacgc aaaaggcgcc
      841 gtcaccgcgg cggcggatct atagtacttt cgttctattg ccgggactaa acgacgggag
      901 tagctttggg aaacacccaa cctatgcagt tgccttccct caaccgctgg cattttattt
      961 ttgcggcaac ggctttcttt gcctattcca tgaccggatg ttcgaagaac tcgaacccca
     1021 ataacaaccg caacgattcc gacacggtcc tccaccgaac cgtctacgac agcgcgaaat
     1081 tctatatcac gtttgatatg gagattgcaa aaaccagcgg tgtctttaac gatacgttct
     1141 ccgatcatgc cagcatgatc gtctatgtcg tgaacggcgt cgtaaaggtg ccacccgaca
     1201 gtctgcgtaa ttttccaccc atcgtttacc cggcgtcggg cagctcgggc gcgtggtcgg
     1261 ctacctggat ccccgataat atcggcgaga tcaatgtcac cggtgcaaca ggcctcgtga
     1321 ccgccgatac cgcggttgcg ttgatcctca cacaaacggg atgcgtcacg cctgactgga
     1381 acatctcatt tatgggtggc gccccctctt ccacgggcaa caatccgtcg ccgggctggc
     1441 ccctcgtttt gacctttaac ccaaaactaa aaagccagta tccattcaag ctggatcagg
     1501 cgggtagctc atgggcaatc tggtgctaca aagattattg agaaggttca gggattattg
     1561 aggaggttca gggctccgtc agcaccacaa attcgcccca caccggcgac tggtcataga
     1621 ggtcccggat aacggcgggg ccataggccg caaaccaggg caggatattc tccacccgct
     1681 cttgcaagct gccatgtggg aacagggctt ccttcaccgc gtggatctgc cgttgctggt
     1741 cgccaaattt ccgtttctcg gcccgcagca gcttcttctc cagcaaccgg atcggctcga
     1801 cggcctttgt ctgcaaggcc gccacatgct gttccagcgt cggatcgaca ggtctcgcca
     1861 gcgccttaag cgactcgtag tagtgattgg ccgtggcgat ctcttcttcc aggctgagcg
     1921 tctgggtcga atcccgcctt accagttggt tcaaaaggtc ctgttcgtca cggaaaagat
     1981 cggtgatgcc aaaacccgcc cgctccatct tctccatcca ctgccgttgc accagcagaa
     2041 aagagttgcg cagcacaagc acgggaaacg gggtttgata atgatcgaaa agccccttca
     2101 actcgagcca gtaggccgtt tccccaccgc cgccgacaaa cgcaagattg ggcaggatcg
     2161 tctcctggaa caagccccgc aggatcacgt tggggctgaa ccgctcgggg taacggtgca
     2221 gctcatcccg caactcgtcg gccgtaaagg taaaggaaga accatggatc ttataaacgt
     2281 cacccacctg tccgatcctg ccgcgcagct cgtcttttag ataaaaaagg ttgatgtccc
     2341 gcggattggc ctgtatatca tagtgctcgc ccaatcgttc gatactgtag ctgacgatcc
     2401 tcgacgactc ctgccggaaa agatcgtcct cgaaaaccgc gatcatctgc cgcttgaacg
     2461 cgggccggtc ggc
//
LOCUS       DATGFG010000687         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1161444, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000687 DATGFG010000000
VERSION     DATGFG010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161444"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VKQ52_12820"
     CDS             <1..389
                     /locus_tag="VKQ52_12820"
                     /inference="COORDINATES: protein motif:HMM:NF019478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88126.1"
                     /translation="SAALCINTVRTFYLVILAILGPLAFAFSVYRGLEDSLTQWLSRY
                     LHVFLWLPVANIFGSLIAQIQQEMIRLDIAQLSATGQTTFGATDAAYLVFLLMGIVGY
                     FTVPSVTHYIIRSAGMGARLWRTSLT"
     gene            402..1019
                     /gene="traK"
                     /locus_tag="VKQ52_12825"
     CDS             402..1019
                     /gene="traK"
                     /locus_tag="VKQ52_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraK"
                     /protein_id="HLZ88127.1"
                     /translation="MFPKTRNLETAFQHVRSFTILVVAGSLLLCGFVIWQALRAIDRN
                     QDRIYILESGRALEAMAGSRRENIPVEARDHIRTFHEDFFTLDPDEKLIAAHLGKALY
                     LADGSARRVYEGLKESGYYNGIIAGNISQEICIDSIALEMHQYPIAFRCYATERIIRP
                     TSLVTRNLVTEGWLRNTGRSDNDPHGFLIERWTILDNHDLKVETR"
     gene            1045..2598
                     /gene="traM"
                     /locus_tag="VKQ52_12830"
     CDS             1045..2598
                     /gene="traM"
                     /locus_tag="VKQ52_12830"
                     /inference="COORDINATES: protein motif:HMM:NF023922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraM"
                     /protein_id="HLZ88128.1"
                     /translation="MKLTITKEKRRFLLWLPLVAFPFLCLVFYTLGGGRGSAKDKKPA
                     IMGLNTQLPPLPFDLQKAFQDKLKTYEQVDNDSLRKAQYQRQDPYHKDSVTTSVTTTR
                     RQTPSAKPAIVAPFADPRAGQLEQQLARLQQSLHQKQPVFHPAPPPHYDDAASMPDKP
                     APDPQIDRLNAMLDKVIRIQHPQEQPPATPTPPAHPADEVLPADTNANSIAAIIPEDQ
                     TLTTGVTIALRITDSIRISGRTLSAGELVYGTVTLNNDRLLVHIGSLREGRNLYTTDW
                     QVYDLDGLPGIHIPGMIGRDVAKQSADQGVNALNVMTLDPSLGAQAAGAGIQAAKTFL
                     GRNVRQVRVTVRAGYQVLLRNPRASDSRQKRPLPPAIKTDSIQAPGFEPGSPVLVHCR
                     TEGMELRLRHLLIRDSCLWFGVEWRNHSPIVYTPAYARWYIRDRRVFRRTALQEQTLE
                     PVYPTVLPIVGNDSAEHSFTGFRPFALAKDKELVLEVGEKGGGRTLILVIPSKQILKA
                     KNDEKGNQP"
     gene            2576..2908
                     /locus_tag="VKQ52_12835"
     CDS             2576..2908
                     /locus_tag="VKQ52_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88129.1"
                     /translation="MKKETSHKRGEVKVRYTAEDFIRVKKAFARSTCTTIAGYVRKIS
                     LGEPIEIITRNASFDTFIEEIVLLRKEMAVLRTQINLSPADQANLFRLHEHIQQTIDK
                     IAALCTPQ"
     gene            2893..>3141
                     /locus_tag="VKQ52_12840"
     CDS             2893..>3141
                     /locus_tag="VKQ52_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88130.1"
                     /translation="MHASIGFNRSIAHVLDYNEQKVEQNLGECIWAENFARDYTSLTY
                     NEKFHRFQQRASLNDTIKRNMYHTALRFGATEHLSNDKM"
BASE COUNT          724 a          980 c          832 g          605 t
ORIGIN      
        1 aatcggcggc gctttgcatc aacacggtgc gaacctttta cctcgtcatc ctggccatcc
       61 tgggcccgct ggcctttgcc ttttcggtct acaggggatt ggaggatagc cttacgcaat
      121 ggctctcgcg atacctgcat gtctttctct ggctgccggt ggcaaacatc ttcggttcgc
      181 tgatcgcgca gatccagcag gaaatgatca ggctggacat cgcgcagctg agcgccacgg
      241 gtcagaccac ctttggcgcc accgatgctg cctacctggt gttcctgctg atgggcattg
      301 tcgggtactt caccgtaccg tcggtaacgc attatatcat ccgtagcgcg ggcatgggcg
      361 cccggctctg gcggacgagt ctcacctaaa aaatgaatgc tatgttccca aaaacaagaa
      421 atctcgaaac ggcctttcag catgtccgga gttttaccat cctggtggtg gcgggcagcc
      481 tgctgctctg tggttttgtc atttggcagg ccctgcgggc gatagatcgg aaccaggacc
      541 ggatctatat cctcgaatcc ggcagggcgc tggaggcgat ggccgggagc cggcgggaga
      601 atatccccgt ggaggcccgg gaccatatcc gcacttttca cgaggacttc tttaccctcg
      661 accccgacga gaagctgatc gccgcccacc tgggcaaggc cctctatctc gccgacggct
      721 cggcgcggcg ggtgtatgag gggttgaagg agagcggcta ttacaacggc atcatcgcgg
      781 gtaatatcag ccaggagatt tgtatcgaca gcatcgcgct ggagatgcat cagtacccga
      841 tcgccttccg ctgttatgcc accgaacgga tcatccggcc gaccagcctg gtgacgcgca
      901 acctcgtgac ggagggctgg ctgcgcaata ccgggcgaag cgataacgat ccgcacggat
      961 ttttgatcga gcggtggacg atcctcgata accatgatct gaaagtagaa acccgttaat
     1021 ctgaccaaac ataaatcaaa acatatgaaa ctgaccatca ctaaagaaaa acgccgcttc
     1081 ctgttatggc ttccactcgt tgccttcccc tttctctgtc tcgtcttcta taccctggga
     1141 ggcggccggg gatcggcaaa agacaaaaag ccggccatta tgggcctcaa cacccagttg
     1201 cctccgctcc ctttcgatct ccaaaaggcc ttccaggaca agttaaagac ctacgaacag
     1261 gtcgacaatg attcgctgcg caaggcgcaa taccagcggc aggacccata tcataaggat
     1321 agcgttacta ccagcgttac taccactcgc cgccagaccc catccgcaaa gcccgcgata
     1381 gtcgctccct ttgccgaccc ccgcgccggc caactggagc agcaactggc ccggttacaa
     1441 caatccctgc accaaaagca accggtgttc cacccggcgc cgccgccaca ctacgatgac
     1501 gcagcttcta tgccggataa gccggcccct gatccgcaga tcgaccggtt aaacgcgatg
     1561 ctcgacaagg tcatccgcat ccagcatcca caggagcaac cgccggcgac cccaactcca
     1621 cccgctcacc cggcggacga ggttcttccc gccgacacga atgccaatag cattgcggcg
     1681 atcatccccg aagaccagac ccttaccacc ggtgtgacta tcgccctgcg catcacggac
     1741 tcgatccgga tcagcgggcg gaccctttcc gccggtgagt tggtttacgg gacggtaacg
     1801 ctcaacaacg accggctgct tgtccacatc ggctccctgc gcgagggacg caatttgtac
     1861 accaccgatt ggcaggtgta cgacctggat ggattgccgg gtattcatat tcccgggatg
     1921 ataggtcgcg acgtggcgaa acaatccgcc gaccaggggg tcaatgcact caatgtaatg
     1981 accctcgatc ccagcctggg cgcgcaggcg gccggcgccg gtattcaggc agcgaagacc
     2041 ttcctggggc gcaatgtgcg gcaggtgcgg gtgacggtaa gggcgggtta ccaggtattg
     2101 ctgcgcaatc cccgggcgtc agattcgcga caaaaacgtc cgttgccgcc ggccatcaaa
     2161 accgacagca tccaggctcc cggcttcgag ccgggctccc ctgtgctcgt gcattgccgc
     2221 accgaaggga tggagctacg cctgcgccat ctgctcatcc gggactcctg tctctggttc
     2281 ggggtggaat ggcggaatca ttcgccgatc gtttatacgc ccgcttatgc ccgctggtat
     2341 atccgcgatc ggcgggtgtt caggcggacg gccctccagg agcaaaccct cgaaccggtc
     2401 tacccaaccg ttctccccat cgtcggtaac gattcggcgg aacacagctt caccgggttc
     2461 cgacccttcg ccctggcaaa ggacaaggag ctcgtgctcg aagtcggtga gaaaggcggg
     2521 ggcagaaccc tcatactcgt catcccatca aaacaaatcc taaaagccaa aaacgatgaa
     2581 aaaggaaacc agccataaac gcggcgaagt caaagtccgc tacacagccg aagacttcat
     2641 ccgggtgaaa aaggcattcg cccgctccac ctgcacgacc attgccgggt acgttcgcaa
     2701 gatctccctg ggggaaccca ttgagatcat cacccgcaac gcttcattcg acactttcat
     2761 cgaagagatc gtgctcctgc ggaaagagat ggcagtcctc cggacgcaaa tcaacctttc
     2821 cccggcggat caggccaacc ttttccggct ccatgaacac atccagcaaa ccatcgacaa
     2881 aatagcagcc ctatgcacgc ctcaataggc ttcaaccgca gtatcgccca tgtcctggac
     2941 tacaacgagc agaaggtaga acaaaacctg ggcgaatgta tctgggcgga aaattttgcg
     3001 agggactaca catccctcac atacaacgaa aaattccacc gttttcaaca acgagcctct
     3061 ttaaatgaca cgataaaaag aaatatgtac cacaccgcgc ttcgctttgg cgcgacggaa
     3121 catctttcga atgacaagat g
//
LOCUS       DATGFG010000688         4995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_966671, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000688 DATGFG010000000
VERSION     DATGFG010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966671"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VKQ52_12845"
     CDS             <1..276
                     /locus_tag="VKQ52_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88131.1"
                     /translation="KPGLFVCVAAFLRGECIHYIIRDNGIGREQAGAHKQQNRPHHKS
                     IGLTITEDRIHIFSHQHESEGSVTITDLLDEKGQAAGTRVEVIIKAV"
     gene            278..1021
                     /locus_tag="VKQ52_12850"
     CDS             278..1021
                     /locus_tag="VKQ52_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ88132.1"
                     /translation="MEPIKAILIDDERSSLENLAQKIREFCPEIKILATVEQPTEALF
                     LIRHHRPDVIFLDIEMPHLNGFRMLNELGDYAAEIVFTTAYNHYAIEAIRISAFDYLM
                     KPVSINDLRETVTRLTKQLSKATQQRLDVLRQSLNKTGSQDQKIAVPTWEGLEFILIK
                     NVVRIESSSNYSRLFFINGESLLVTKQLKEFEELLVPYRFCRVHNVHLINLNYVSKYI
                     RGEGGSVIMENGDEIDVSRRKKDDFLRQI"
     gene            complement(1022..3412)
                     /locus_tag="VKQ52_12855"
     CDS             complement(1022..3412)
                     /locus_tag="VKQ52_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88133.1"
                     /translation="MLRNYFRIAFRNLSRNRFSTAINIGGLAIGMAVAILIGLWIYDE
                     LSFDHVHRNHDRIAAVMQNQKLSGGIQTWQGQAMQLAPALRKDYGADFKYVVTEAGIR
                     RQLLSYGDKKMKISGDYMEPQGIDMLSLKMVRGNNSALDDPATVILSESTVKDLFGDQ
                     DPMGKTIRLDNKDLVKVTGVYADLPANSTFADVNYICPFALLVRLQDLNTRVNWGNSW
                     FQVYVQLNDHIDMAQASKAIKFVKRDNSPGDRRFNPELFLHPMNHWHLYSDFEGGVSV
                     GGRIGYVRLYAVIAVFVLVLACINFMNLSTARSEKRAKEVGIRKAIGSLRGQLIRLFY
                     TESLVIALLSFLLSIGIVGLLLPFFNEVADKKIGLPMGQPVFWLAGLCFTVFTGLLAG
                     SYPALYLSSFNPVKVLKGTFKAGKLAALPRKVLVVVQFTVSVILIIGTLAVLRQIEYV
                     KDRPVGYERAGLLFIPRQNLDAKTHLQALTDDLLKTGLFSGVAGSESSITNTYINNMG
                     FDWKGKDPSLQESFITNGITPEFGKVNGWQIVEGRDFLPNYATDSGNILVNETAVQYM
                     GLHHPIGEVVKWGDNGRYTIIGVVKDMISQSPYDRMTPMLFYLSSALSFSTINNIDIR
                     VKPRASMSRALAVIQAVFKKYDPEDPFQYTFADQDYAKKFGDEERIVQLAGFFTILAI
                     FISCLGLLGLSAFVAEQRTREVGIRKVLGASVVHLWNLLSREFVWLVGISLLVGGPVA
                     YWIMHGWLNNYQYHAPLSWWIFALTAMGAIGLTLFTVSFQAIRAAVSNPVKSLRSE"
     gene            complement(3427..>4995)
                     /locus_tag="VKQ52_12860"
     CDS             complement(3427..>4995)
                     /locus_tag="VKQ52_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ88134.1"
                     /translation="IEANGSSLYLLIFGALALFILLLACANFTNLSTAGAAARGKEVG
                     IRKVMGSVRKQLIFQFLAESILLTTVSMIAAFGIVFLLLPYFNGVSGKHFTYNTFLTW
                     TSLAGAFGLTLAVGALAGIYPAFFLSSFSPVKVLKGAVQGSRRSLLRSSLVVFQFVVS
                     IALIVATLIVYRQLHYMQEMRLGYDKEQVLYIQDTFLLDNNQEAFRQELLGDKRVANA
                     SISWCVPGSGAMGGTEIYPKAEGPNDAKGEIHTNIYNIDYDYVATLGLQVVKGRSFSR
                     DFPTDTANGVLINETAAADLGWGHTDPIGKVIVRSGRKEYKVVGVVKDFHYLSARERI
                     APLMLLLGNNAGGIMVKVKTADVSDFLAATQQRWEAFHPKGPFSYFFLDDRFARLYVA
                     EQRTGRLFTAFTLIAVLIAGLGLFGLAAYMAEQRTREIGIRKVLGATVGSVLVLVSKE
                     FLLLVGLAFLIAVPVTWWAMDRWLREFAYRTPVSWWIFPLAGVAALVIAILTISFQAT
                     KAATANPIKSLRSE"
BASE COUNT         1187 a         1459 c         1395 g          954 t
ORIGIN      
        1 aagccgggac tcttcgtctg cgtagccgcc tttctccgcg gagagtgtat ccattatatt
       61 atcagggaca acggcatcgg ccgcgagcag gccggcgccc ataaacagca gaaccggcct
      121 catcataaaa gcatcggcct caccatcacc gaagaccgca tccatatctt tagccaccag
      181 cacgagagcg agggcagcgt caccatcacc gatctgctgg atgaaaaggg acaggccgcg
      241 ggcacaaggg tggaggtcat cataaaagcc gtttgacatg gaacccataa aagccatcct
      301 catcgacgac gaacggagca gtctcgagaa cctcgcgcag aagatccgcg aattctgccc
      361 ggagataaag atcctggcga cggtggagca gccgacggaa gccctcttcc tcatccgcca
      421 ccaccggccg gacgtgatct tcctcgacat cgagatgccg catctcaacg ggttcaggat
      481 gctgaacgaa ctgggcgatt atgcggcgga gatcgtgttc acgacggcgt ataatcatta
      541 tgcaatagag gcgatccgga tcagcgcctt tgactacctg atgaaacccg tgtcgataaa
      601 cgatctccgc gagacagtga cccggctgac aaaacaatta tcgaaggcga cccaacaacg
      661 gctcgacgtc ctgcgccaat ccctcaacaa gaccggcagc caggaccaga agatcgccgt
      721 ccccacctgg gaggggctcg aattcatcct gataaagaat gttgtccgga tagaatccag
      781 ctccaattac tcccgcctct tctttatcaa cggcgagtcg ctgctggtga caaaacaact
      841 gaaggagttc gaggagctgc tggtccccta ccgtttctgc cgcgtgcaca acgtgcacct
      901 catcaacctc aactatgtca gcaaatacat ccgcggcgaa ggcggaagcg tgatcatgga
      961 gaatggggac gagatagacg tgagccgccg gaagaaagac gatttcctgc gacagatctg
     1021 atcactcact gcgcaggctc ttcaccggat tgctcaccgc cgcccggatg gcctggaagc
     1081 tcaccgtgaa cagggtcaac ccgatcgcac ccatcgccgt cagcgcaaag atccaccacg
     1141 acaggggggc atggtattgg tagttgttta accatccatg catgatccaa tacgcgacag
     1201 gaccacccac caacaacgag atccccacca gccatacaaa ctcccgcgac aacaaattcc
     1261 agagatgcac aacggaggcg ccgagaactt tgcggatccc cacctcccgc gtgcgctgct
     1321 ctgcgacaaa ggccgaaagc ccaagcaacc cgagacagga gataaagatg gccaggatgg
     1381 taaagaagcc cgccaattga acgatccgct cttcatcgcc gaacttcttt gcatagtcct
     1441 ggtcggcgaa agtatattgg aaggggtctt cgggatcgta tttcttgaat acggcctgga
     1501 tgacggcaag ggcccggctc atgctggccc ggggttttac ccggatatcg atattgttga
     1561 tggtcgagaa ggaaagcgcg gaggagagat aaaagagcat gggagtcatc cggtcgtaag
     1621 gcgactgcga gatcatatcc tttaccacac cgatgatcgt ataccgcccg ttatctcccc
     1681 attttactac ttcgccgatg ggatggtgca agcccatata ttgcaccgcc gtctcgttga
     1741 cgaggatatt accggagtcc gtggcataat tgggcaggaa gtcgcggccc tcgacgatct
     1801 gccagccgtt gacctttccg aattccgggg tgatgccatt ggtgataaag ctctcctgta
     1861 acgacgggtc cttccccttc cagtcaaaac ccatattgtt gatataggtg ttggtgatac
     1921 tgctctccga gccggccaca cccgagaaga gcccggtctt caggagatcg tcggtcaacg
     1981 cctgtaaatg cgtttttgca tcgaggttct gccgcgggat aaagagcaac cccgcccgct
     2041 cgtaacctac cggccggtcc ttgacatact ctatctgccg caggaccgca agggtgccga
     2101 tgatgaggat gacgctgacg gtgaattgca ccaccaccag caccttgcgc ggcagcgcgg
     2161 ccagtttgcc cgccttgaag gttcctttca gcactttcac ggggttgaag gaggacaggt
     2221 acagcgccgg gtagctgccg gccagcaggc cggtgaagac ggtaaagcaa agtccggcga
     2281 gccagaagac gggctggccc atcggcaggc cgatcttctt atcggctact tcattgaaaa
     2341 aaggtaagag caatcccacg ataccgatcg agagtaggaa ggaaagcagg gcgatcacca
     2401 gcgactcggt atagaacagg cggatcaact gcccgcgcag ggaaccgata gccttgcgga
     2461 tccccacctc tttcgcgcgc ttctcgctcc gggccgtgct caggttcatg aaattgatac
     2521 aggccaggac caggacgaac accgcgatca ccgcgtagag ccggacatag ccgatccggc
     2581 cgccaacgct tacgccgccc tcgaaatcgg aatagagatg ccagtggttc atgggatgga
     2641 ggaagagctc cgggttgaaa cgccggtcgc cgggggaatt atcgcgtttg acaaatttga
     2701 tggctttgga ggcctgcgcc atgtcgatat ggtcgttgag ctggacatac acctggaacc
     2761 agctattgcc ccaattcact cttgtattga gatcctgcag tcggaccagt aaggcaaaag
     2821 gacagatata attgacatcg gcaaaggtgg aattcgcggg caggtcggca tagacaccgg
     2881 tcacctttac gagatctttg ttgtcgagcc ggatagtctt gcccattggg tcctgatcac
     2941 cgaagagatc tttgaccgtc gactcggaaa ggatcacggt agcggggtca tcgagagccg
     3001 aattgttgcc ccttaccatc ttcagggaga gcatatcgat cccctggggc tccatatagt
     3061 cgccggagat cttcattttc ttgtcgccat agctgagcaa ctgccggcgg atccccgcct
     3121 cggtgacgac gtatttgaag tcggcgccat agtctttgcg cagtgcggga gcgagctgca
     3181 tggcctggcc ttgccaggtc tggatccctc cactgagctt ctggttttgc atcaccgcgg
     3241 cgatccggtc gtggttgcgg tgaacatggt cgaacgaaag ctcgtcgtag atccacagac
     3301 cgatgaggat ggccacggcc atcccgatag cgaggccgcc gatgttgatg gcggtggaga
     3361 agcggttccg gctgagattg cggaacgcaa tacggaaata gtttctaagc atggagacgc
     3421 attttattat tcgctacgca atgattttat tggattggcc gtcgcggcct tcgtcgcctg
     3481 gaaactgatc gtcaggatcg cgatcaccaa cgcggctacc cccgccagcg ggaagatcca
     3541 ccagctcacc ggcgtacggt acgcaaattc ccgcaaccag cgatccatgg cccaccaggt
     3601 gaccggtacc gcgataagaa aggccaggcc caccagcaac aggaactcct tcgacaccag
     3661 gacaagtacc gaacccaccg tagcgcccaa caccttgcgg atgccgatct cccttgtccg
     3721 ctgctcggcc atataggccg caagcccgaa gagcccgaga cccgcgatca gcaccgcgat
     3781 aagggtaaag gccgtaaaga gccggcccgt ccgctgctcg gcgacgtaga gacgggcgaa
     3841 gcggtcatcg aggaagaaat aactaaacgg ccccttggga tggaaggcct cccagcgctg
     3901 ttgggtcgcc gccaggaagt cgctcacatc ggccgtcttc accttcacca ttatcccgcc
     3961 tgcgttgttg ccaagcagga gcatcagcgg cgcgatcctt tccctggccg agagataatg
     4021 aaaatccttc accacgccga ccaccttgta ttccttgcgc ccgctccgca caatgacctt
     4081 cccgatcggg tcagtatgac cccaccccag atcggcggca gccgtttcgt tgatcagcac
     4141 tccattcgcg gtatcagtcg ggaaatcccg ggagaagctc cgccccttca ctacctgcag
     4201 accaagcgtg gcgacatagt catagtcgat attatagata ttggtatgga tctcgccctt
     4261 tgcgtcgttg ggcccctccg ccttgggata gatctccgtt ccgcccatcg cccctgaacc
     4321 cggtacacac caactgatac tcgcgttggc gacccgtttg tccccgagca attcctggcg
     4381 gaaggcttcc tggttattgt cgagcaggaa agtatcctgg atataaagca cctgctcctt
     4441 gtcatagccc aaccgcattt cctgcatata atgcagctgg cggtagacga tgagcgtggc
     4501 gacgatcagc gcgatggaga cgacgaattg gaagacgaca agactgctgc gcaacaggct
     4561 tcgacggctg ccctgcacgg cccctttcag caccttcacc gggctgaagg aagaaaggaa
     4621 gaaggccgga tagatgcccg ccagggcgcc gaccgcaagc gtcagcccaa aagcgcctgc
     4681 cagggaggtc caggtaagaa aggtgttata ggtaaaatgc tttcccgaaa ccccgttgaa
     4741 atagggcagg agcaggaaga cgataccgaa ggcggcgatc atggaaacgg tggtgagcag
     4801 gatcgactcg gccaggaact ggaagataag ctgcttgcgg acggacccca tcaccttccg
     4861 gatacccacc tcctttcccc ttgcggcggc gccggcggta gagagattgg taaaattggc
     4921 gcaggccagc agaaggatga acaaggccag cgcgccgaag ataaggagat acagactgct
     4981 gccattggcc tctat
//
LOCUS       DATGFG010000689         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_754847, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000689 DATGFG010000000
VERSION     DATGFG010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754847"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VKQ52_12865"
     CDS             <1..607
                     /locus_tag="VKQ52_12865"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88135.1"
                     /translation="GVRPFTKLNAETIYQSNFLSYTQCLEANTYPNCIVDSLLYQSYA
                     GNDLRRVLYYTINANGHPNIKFGYSGLAFPFSGLATDECFLIRAECTARAGNAASALN
                     DLNTLLKNRWKTGSFVPVTAASPAEALDIILAERRKELAFRGIRWSDLRRLNKEGRNI
                     TLQRLLNGITFTLPLNSTLYTLPIPPDVLNDNPGMQQNMRY"
     gene            789..2204
                     /locus_tag="VKQ52_12870"
     CDS             789..2204
                     /locus_tag="VKQ52_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88136.1"
                     /translation="MRKLLILCALFIFAFLFFRCRKDRSTVQPLSDTSLIQNARKYFN
                     DSVQASTPVTESGPRITTTRQPLWDSAKTINTAIGKAVIVPIHYDKDLHIRANFAGRK
                     LFNLDELTHLLIYCDPKQRHHAELITGIPDSIALFAGKGGFSGILFVEDWSGHRIKQY
                     KYGPDGSILQTGSAPQTGKGASIINNSNTPVSPDIVYTTCYEIDGYNYSEGDPEDGYS
                     WSESAGCVSMYFPIPASIAPIRSPSAGNYGYIAAPPGRTSVTIAPPDNPIDDIKAYFK
                     CFTSSSGVDHSYTVTVCVSQPVPGTRLPWAFTSGGVTGSSTAGNAFDVGHTFLIFSEN
                     SAGNIVARNVGFYPEAIVNPLYPSDQGILDDNERTPYNISLSFTVTNTQFFNMLNYAV
                     IGNNPGYFYDLNSNNCTTFVLHALAAGGISLASRQGTWPGGGHGYDPGDLGEDIRGMS
                     VPSNAKRNTVQNPHPNQGNCN"
     gene            2232..2639
                     /locus_tag="VKQ52_12875"
     CDS             2232..2639
                     /locus_tag="VKQ52_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88137.1"
                     /translation="MKIMCLVFMIGLTFHSCKAQLQTWSSTRNWKLYPGEGQTVFQFP
                     VDSLKNTRNTLLNQDSIQFYLEKMQPIPPERTPTWMGAYLASYESSDGRVNKVEVGAY
                     GGYFYDVSSDKYFYLPRPFIRPWQKFITRNIPN"
     gene            complement(2664..>2961)
                     /locus_tag="VKQ52_12880"
     CDS             complement(2664..>2961)
                     /locus_tag="VKQ52_12880"
                     /inference="COORDINATES: protein motif:HMM:NF019583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HLZ88138.1"
                     /translation="FYPVTPASDQYALGAPLFPRITLHLENGRTLLIDAPGNSAENRY
                     VQALRLNGHPYTHNWLSHKTLLQGATLDFTMSPHPDRRRGIKEADRPFSMSTAD"
BASE COUNT          753 a          797 c          681 g          730 t
ORIGIN      
        1 cggcgtccgc ccctttacca agctcaatgc cgagacgatc taccagtcca atttcctctc
       61 ttatacccag tgcctggagg cgaacaccta ccccaactgt atcgtcgatt ccctacttta
      121 tcagtcctat gccggtaacg acctgcggcg tgtcctttat tacaccatca atgcaaatgg
      181 ccatcccaac attaaattcg gctacagcgg gctggccttc cccttcagcg ggctggcgac
      241 cgacgaatgt ttcctcatcc gggccgaatg cactgccaga gccggcaatg cagcctcagc
      301 acttaacgac ctcaataccc tgttgaagaa ccggtggaag acgggcagct ttgtacctgt
      361 gacggcggct tccccggccg aagccctcga tatcatcctt gcggaacgaa ggaaggaact
      421 cgccttccgg ggcatccgat ggtcggatct gcgacggcta aataaagaag gaaggaatat
      481 caccctgcaa agactattga acggaataac ctttaccctg ccgcttaaca gtacccttta
      541 caccctacct ataccgccgg atgtgcttaa tgataatccc ggaatgcaac agaatatgag
      601 gtattgacat taagtccgga tgtaggtggg aagccggaaa gaaagaggat tttctgccgg
      661 aggcagttcc ttgctgcgag ggtgcagttg gtttagtagt tacatctatt ggtgatagtt
      721 atcgactatt cgaattgggt ttatgattgg ggttgtttaa attcacataa accttaatcg
      781 tccgtcatat gagaaagctt cttatcctgt gtgcactgtt tatcttcgct tttctatttt
      841 ttaggtgcag aaaagaccga tcaacagttc agccgctatc ggatacttcc ctgattcaaa
      901 atgcccggaa atatttcaat gactccgtcc aggcctctac tcccgttaca gaatcaggtc
      961 ctcgcatcac gaccaccagg cagccccttt gggactctgc aaaaacgata aatacggcaa
     1021 tcggcaaagc cgtaatagtc ccaattcact atgacaagga ccttcatatc cgcgcaaatt
     1081 tcgctggccg gaaactattt aacttagatg aacttaccca tttgcttatt tattgcgacc
     1141 ctaaacaacg ccaccatgcc gaattgatta ccggtattcc cgacagcatt gcgctatttg
     1201 cgggtaaagg cgggttctcc ggaattctct tcgtcgaaga ctggtctggc catcgaatta
     1261 aacaatacaa atacggccct gatggctcca ttcttcagac tggttcagcc ccacaaaccg
     1321 gcaaaggggc ctccattata aacaattcca acacgcctgt cagccccgac atcgtatata
     1381 ccacctgcta tgaaatcgac ggctataact attccgaagg tgatcctgaa gatggatatt
     1441 cctggtctga atctgccggc tgtgtaagta tgtattttcc cattcctgca tctatcgctc
     1501 ccattagaag cccgtcagca ggtaattatg ggtatatcgc cgccccgcca ggcaggactt
     1561 ctgtcaccat cgccccgccg gacaatccga tcgatgacat caaagcctat ttcaaatgct
     1621 ttaccagttc ttcaggcgtc gatcatagct atacggtaac ggtatgtgta tcccagcccg
     1681 ttccaggaac caggttgccc tgggccttta cctcaggtgg ggttaccggt tcctccacag
     1741 ccggcaatgc ctttgatgtt gggcacacgt tcctcatctt cagcgagaac agcgcaggca
     1801 atatagtagc caggaacgtc ggattttatc cagaagcgat cgtcaacccc ctctatcctt
     1861 cggatcaagg gatattggat gataacgaaa ggacgccata caatatatcg ctcagcttca
     1921 ctgtcactaa tacccagttc ttcaacatgc taaactatgc tgttattggt aacaatcctg
     1981 gatatttcta cgatcttaac agcaataatt gcacgacatt tgtgctgcat gcgttggcag
     2041 ccggtggcat ttctctggct tcacggcagg gaacttggcc aggcggtgga catggatatg
     2101 accctgggga cctcggcgaa gatattcgag ggatgtccgt tccttccaac gccaagagga
     2161 atacggtcca aaatccgcat ccgaaccaag gaaactgcaa ttaatacctt aaagaaaata
     2221 aaacctattg catgaaaatc atgtgtctcg tcttcatgat cggccttact tttcattcct
     2281 gtaaggcaca actccaaacc tggtccagca ccaggaattg gaagctatat cccggcgaag
     2341 gacaaaccgt cttccaattt cctgtagaca gcctaaaaaa cacgcgtaac accttgttga
     2401 accaagattc tatacaattc tacttggaga aaatgcaacc tatcccgccc gaaagaacgc
     2461 ccacctggat gggcgcctac ctggccagct atgaatccag cgacggcaga gtaaacaagg
     2521 ttgaggttgg cgcctacggt ggctacttct atgacgtttc cagtgacaaa tacttttatc
     2581 tgccccgccc attcatccgg ccttggcaaa aatttataac ccgaaatatc ccgaactaag
     2641 atattctaac ctttcaaatc agattaatcg gcggtcgaca tagaaaaggg ccggtcggct
     2701 tcctttatcc cacgccggcg atccgggtgc ggtgacattg taaaatcgag agtggcgccc
     2761 tgcaacaggg ttttatggct aagccaattg tgcgtatagg gatgaccgtt tagcctgagg
     2821 gcctgcacgt agcgattttc ggcgctgttg ccgggggcat cgatgagcaa tgtcctgccg
     2881 ttttccaggt gaagcgttat ccgcgggaac aggggcgcgc cgagggcgta ctggtcgctg
     2941 gccggggtca ccgggtagaa g
//
LOCUS       DATGFG010000690         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1499647, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000690 DATGFG010000000
VERSION     DATGFG010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499647"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /gene="serA"
                     /locus_tag="VKQ52_12885"
     CDS             <1..1150
                     /gene="serA"
                     /locus_tag="VKQ52_12885"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF008759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HLZ88139.1"
                     /translation="KESGYASVRKLGGALSEDQLINEIKDVHLLGIRSKTQITPKVLE
                     AAKKLQAIGCFCIGVNQVDLKAAMNTGVVVFNAPYSNTRSVAELVIGLAIMLIRRIPD
                     KSKAAHEGIWLKEAKGSYELRGKTLGIIGYGNIGTQVSILAEALGMKVIFYDTVTKLP
                     LGNAEAKKTIKDVVSQADIITLHVPDTQQTKNLINKTVIRQFKKGSILLNYARGEVVD
                     LDGLAKALQDGQLSGAAIDVYPWEPEKNGDRFTTPLQGLPNVILTPHIGGSTEEAQEN
                     IGEDVSNKLFQYLETGMTIGSHTIPPLSLPLQEGTHRILHIHKNVPGVLSEINSTLSS
                     HNINILGQYLKTNDEIGYVVLDFDKRLSNQALQLLKEVKQTIKTRLLY"
     gene            complement(1163..1693)
                     /locus_tag="VKQ52_12890"
     CDS             complement(1163..1693)
                     /locus_tag="VKQ52_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88140.1"
                     /translation="MDILQSLKKEHSKKQTDRIVDYIGADSQRFAILIDLFFKGEYRI
                     TQRAAWPLSYCVRAHPELIEPYFKPLLDNLGRKDIHIAVIRNTVRLLQDVDIPKKHQG
                     RVMNICFDFVQSPETPIAVKAFSLTILSHLAKDYPDIRGELKLIIEEQWEHSTPAFRS
                     RAKKVLKAMEPTRATQ"
     gene            1727..2722
                     /gene="mgrA"
                     /locus_tag="VKQ52_12895"
     CDS             1727..2722
                     /gene="mgrA"
                     /locus_tag="VKQ52_12895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HLZ88141.1"
                     /translation="MSYRPNMTRYDAMEYRRCGHSGLLLPAVSLGLWHNFGHVDVLEN
                     CRNILHLAFDSGITHFDLANNYGPPPGSAEENFGRILKEDFHGYRDQMIISSKAGYYM
                     WPGPYGEWGSRKYLVASLDQSLKRMGLDYVDIFYHHRPDPNTPLEETMAALDHIVRQG
                     KALYVGISNYRAEEAAKAIQLLRQLGTPCLIHQPKYSMFERWVEGGLFQLLEKEGVGC
                     IPFSPLAQGLLTNKYLKGIPEDSRAAKPTGFLKEGEVTAEKINKIQQLNELATRREQT
                     LAQMALAWILRDKRVTSVLIGVSRPEQVTDSLQCFRNTWFSEEELRQIENILVAK"
     gene            2871..3350
                     /locus_tag="VKQ52_12900"
     CDS             2871..3350
                     /locus_tag="VKQ52_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88142.1"
                     /translation="MKQFTLYLASLLLCAAAYADPGPVPTPAGNASAANKALVNAGAV
                     RFQFNVAASNDDHQDSVLVIFDRYDHTGAGVVYQMFATDAEHGITIPAVPAGKYYVTI
                     EVRGLHRDHMETLVTVKSKKSEKVKIKLEDSEVFSKDNVKIPVYRPAFSDMTILKTK"
BASE COUNT          814 a          912 c          931 g          752 t
ORIGIN      
        1 caaagagtcg ggatatgcat cggttcgaaa actgggtggg gccttatcgg aagaccaact
       61 gatcaacgaa ataaaggatg tgcacctgct gggtatccgc tccaagaccc agatcacacc
      121 caaggtgctc gaggccgcaa aaaaattaca ggcaatcggt tgtttttgca taggagtcaa
      181 ccaggtagac ctcaaagcgg ccatgaatac cggggttgtg gtcttcaatg cgccctattc
      241 caatacgcga tcggtcgccg aactggttat cggcctggcg atcatgctga tccgccgtat
      301 ccccgacaaa agcaaggccg cccatgaagg catctggtta aaagaggcga agggcagcta
      361 tgaattaaga ggtaagacat tgggaatcat cggatacgga aatatcggca cccaggtgag
      421 catccttgcc gaagcccttg ggatgaaggt cattttttac gatacggtga ccaagctgcc
      481 gctcggcaac gccgaagcca aaaagaccat caaggatgtg gtcagccagg cggatatcat
      541 cacgctccat gtgccggaca cgcagcagac caaaaacctg atcaataaga cggtgatcag
      601 gcagttcaaa aaggggtcga tcctgttgaa ttatgcccgc ggcgaggtag tcgacctgga
      661 cggactggcc aaggccctgc aggacgggca gctttccggg gcggccatcg atgtttatcc
      721 ctgggagccc gagaagaatg gcgaccggtt cacgacgcct ttgcaaggcc tgcccaatgt
      781 gatcctgacc ccgcatatcg gcggttccac ggaagaggca caggagaata tcggggagga
      841 tgtcagcaac aagttattcc aatacctcga gacggggatg acgatcggca gccacacgat
      901 tccgccgctt agcctccctc tccaggaagg tactcaccgc atcctgcata tccataagaa
      961 tgtgcccggg gtgctttcgg agatcaacag cacgctttcc agtcataaca tcaatatcct
     1021 cgggcagtac ctgaagacca acgacgagat cgggtatgtc gtgctggatt tcgacaagcg
     1081 cctgtccaac caggccctgc aattgctgaa agaagtgaag cagacgatca aaacaagact
     1141 gttgtattaa tggaaaaggg gatcactgtg tagcccgtgt gggctccatc gctttcagga
     1201 ccttttttgc ccggctgcgg aaggctgggg tcgaatgttc ccattgttct tcgatgatca
     1261 gcttcaactc accgcgaatg tcgggataat cctttgccag gtgggagaga atggtcaggg
     1321 aaaaggcctt gacggcaatg ggtgtttccg gggactggac aaagtcgaaa cagatattca
     1381 tgacgcgccc ctggtgtttt ttggggatgt cgacatcctg caacaggcgc acggtattgc
     1441 ggatgacggc gatatggata tccttccggc cgaggttgtc gagcaggggt ttgaaatagg
     1501 gttcgatcag ctccggatgc gcccggacac aatagctgag cggccacgcc gcccgctggg
     1561 tgatgcggta ctcgcctttg aagaacaagt cgatcagtat ggcaaaccgc tgactgtcgg
     1621 cgccgatata gtccacgatc cggtcggttt gttttttgct gtgttctttt ttaagggact
     1681 ggagtatgtc catagttacc taaatttagg gcataatttc ttcgatatga gctacagacc
     1741 caacatgacc cgatatgatg ccatggaata ccggcgttgc ggccacagcg gcctcctgct
     1801 gccggcagta tcccttggcc tctggcataa tttcggtcat gtggacgtcc tcgaaaactg
     1861 ccgcaatatc ctgcacctgg cctttgactc cgggatcacg catttcgacc tcgccaacaa
     1921 ctacgggccg ccgccggggt ccgcggaaga gaatttcggt cggatcctga aggaagattt
     1981 ccatggctac cgcgaccaaa tgatcatttc gtccaaggcc ggttactata tgtggcccgg
     2041 gccctatggc gaatgggggt cgaggaaata cctggtggcc agcctggacc aaagcctgaa
     2101 gcggatgggg ctcgattatg tcgatatctt ttatcatcac cggcccgatc ccaatacgcc
     2161 gctggaggaa acgatggcgg ccctggacca tatcgtccgc cagggcaagg cactttatgt
     2221 aggcatctcc aattaccggg cggaagaagc cgcgaaagcg atccagctgc tccggcaact
     2281 ggggacgccc tgtctgatcc accagcccaa gtattcgatg ttcgagcggt gggtggaagg
     2341 cggcctgttc caactgctgg aaaaagaagg cgtcggctgc atcccgttct cgccgctggc
     2401 gcaggggctg ctgaccaaca aatacctgaa aggcatcccg gaggattccc gggcggcgaa
     2461 acccaccggt ttcctgaagg aaggcgaagt caccgcggaa aagatcaata aaatacagca
     2521 gttgaatgag ttggctaccc ggcgggaaca gacgctcgcc cagatggcgc tggcctggat
     2581 cctgcgggac aaacgggtca cgtccgtcct tatcggggtg agccggccgg agcaggtcac
     2641 cgactccctg caatgcttcc gaaatacgtg gttctcagag gaagagctta ggcagattga
     2701 aaatatactg gtggccaaat aagtggttat tattacctgt ttctgttttc gggacccgat
     2761 cttcccacca tgggaagcat gcaataggaa caaatacgta atacacacat tttcaactgg
     2821 atacaaattg gcatctcgat tggcatatat cagtcaaatc gttgcctttt atgaaacaat
     2881 tcaccctcta tctcgccagc ctgctgctct gtgcagccgc ttacgccgat ccggggcccg
     2941 tacctacccc cgccggcaat gcttcagccg ctaacaaggc cctcgtcaat gcaggcgccg
     3001 ttcgtttcca gttcaatgtt gctgcttcca atgatgatca ccaggattcg gtgctggtca
     3061 ttttcgatcg ttatgatcac accggcgccg gtgtcgttta ccagatgttc gcgaccgacg
     3121 ccgagcatgg catcacgatc cccgctgttc ccgccggtaa gtattatgtt acgatcgagg
     3181 tccgtggcct gcaccgcgac catatggaga ccctggtcac cgtcaagtcg aagaaaagtg
     3241 aaaaagtaaa gatcaagctc gaagattccg aagttttttc caaggacaat gtgaagatcc
     3301 ccgtttaccg tcctgctttt tccgacatga ccattcttaa gacgaagtaa tttccctccg
     3361 atatccgctc tcttttttcc gatccgctat tctttcttta gtttcccga
//
LOCUS       DATGFG010000691         5894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_892230, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000691 DATGFG010000000
VERSION     DATGFG010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892230"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            675..1316
                     /locus_tag="VKQ52_12905"
     CDS             675..1316
                     /locus_tag="VKQ52_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLZ88143.1"
                     /translation="MDVVTSLVVILAIFPWLFPLLFVLIKLDSKGPVFFRQKRVGFLG
                     KTFWCYKFRTMYVNDYADTRRASRNDPRVTRVGWFLRNTCLDEVPQFFNVLLGHMSIV
                     GPRPHMLKDSNEFADLVSNYQFRNLARPGITGLSQVRGYRGPATSFESIFRRYQWDSY
                     YVRNVSFTLDLKIMVSTALLMMRSVFYRDQPVPLDNNRPVYNSESLVSAKKIA"
     gene            1513..1722
                     /locus_tag="VKQ52_12910"
     CDS             1513..1722
                     /locus_tag="VKQ52_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88144.1"
                     /translation="MGDSPRKFMGISGNKGRLANFPDGEFLPPLAAAGDGACTTSTAT
                     SNPSLTYRALTARAVDHAGSLFGHT"
     gene            1740..2225
                     /locus_tag="VKQ52_12915"
     CDS             1740..2225
                     /locus_tag="VKQ52_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88145.1"
                     /translation="MKGFLLSIVAFLLIFLVLNFGYYQEWFRSKPVQYWSDFLKEKGD
                     TSSEFAIKMQRYGFGYTVSIRVQDAMQKKKVAHPVILFEPNSYYRDSLHIYPNIRAPE
                     PAVFYYYTGMEGVWTNSPDVNRANFLLRITKKGAGLDTIKSPQQLQEILARYKKYPPI
                     L"
     gene            2230..3786
                     /locus_tag="VKQ52_12920"
     CDS             2230..3786
                     /locus_tag="VKQ52_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88146.1"
                     /translation="MQLLAFFLLLLCYLLTGYGLLYLFKMRLKAAYTVTLSLLLGLAV
                     ASFLPFLLQLCYVEITPATVFGILALACLVINLPLLDHYRKEGFAAFRRSLPRAGFQV
                     RPYEIPFWVIIGFFIFVSVWRCYYLPPTSRDALSGPEAIAEVAVREHTLINSFFGIDL
                     WTTNNQFKSPFLISLQVIYKMAGFPFGQVWLSIVFISITVFIYHALREKIHPLLAGLL
                     LLLFTMSPEMYAYTFMILYDYSNMVFFFLGIFFLFRYLRDGGTNQLYFAGLLMGIATY
                     IRSETLALALLFLPLLLLVQWRKKMPVKNIALGNGLFLLPSLLGYYLPSQLYIKYYLP
                     VHYDIGGLVNNHLSSLQPLFQRYGDIVTRLLAGEFAIHLWGYFFYFAALLFLAELVFV
                     RRFSREARNWLYAVLVLYLGLGVLGWLLPMINLNETTKRAMFKMLPLVLFYLANNELL
                     IRLSNVISRWEEAPFRGSLAGATAGRPAPASAAPPKSVLTRSGKTKPAAPPAKQRNNP
                     PAGPSKKKKK"
     gene            3963..4421
                     /locus_tag="VKQ52_12925"
     CDS             3963..4421
                     /locus_tag="VKQ52_12925"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HLZ88147.1"
                     /translation="MCADKHHHEQAPRRPNLILSILRNRCSRCREGRLFIDANAYHLK
                     RFMKMNEHCPVCGQPFEIEPGFWYGTGFVSYALAVAVSVASFIAWKVLIGMSLQDNRL
                     FWWMGVNGLLLVLLQPLLMRLSRAIWLNFFVWYDPEWRSRIVRLGRGTFL"
     gene            4491..>5894
                     /locus_tag="VKQ52_12930"
     CDS             4491..>5894
                     /locus_tag="VKQ52_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HLZ88148.1"
                     /translation="MLVKTFGSAVYGVEAITITVEVNVTEGLHTFIVGLADSAVKESI
                     QRVESAIKSNGFYMPRTKIIVNLAPAGIRKYGSAFDLPIAIGILAASEQIPDAELLKD
                     YVIMGELGLDGSIHPIKGALPIAVQARKEQFRGLIVPQQNASEAAIVNQLQVYSFGHL
                     REVIGFFRDSSSTKAVVVDARASFFSQSPLPDLDFSEVKGQENIKRALEIAAAGGHNA
                     IMVGPPGAGKTMLAKRLPGILPPLSLQEALETTKIHSVAGKLPVHATLIAQRPFRSPH
                     HTISGMALVGGGTIPQPGEISLAHNGVLFLDELPEFSRSTLEMMRQPMEDRKVLISRA
                     ALSVEFPSSFILLASMNPCPCGYYNHPDKECTCPPGAVSRYLNKISGPLLDRIDLHVE
                     VVPLSYAQLSDRTSGESSQQIRQRVIAAREIQRRRYEGISPQAMGITTSAHSGIYCNA
                     QIGGRLLRHYCRIDTHGE"
BASE COUNT         1240 a         1658 c         1510 g         1486 t
ORIGIN      
        1 gcactatacg gctatgcaga cgctctcctt cctgcttagc aagttcatcg tcatcgatga
       61 tgtgcctgcg gcgacggctg ccatcaccac ggagtggatc gagcctaaaa atgtacttaa
      121 ctaattttta acttcccttc cttttttttc ggcattcccg agcgtgtcag ttttacggtt
      181 ttccgtgttc tccgcggtca ggggcgtgta aaaaagccca aaactgtgtt aaaaccccgc
      241 tcatggcccc atcggcggtt tttttaatta acagactcta atgcaacgtt ttttagatac
      301 cccttgtcaa aggcctcgtg gataagcttt acccccgacc tccggtagga aatcctgccc
      361 tcggaaaccg acccggatgt ggatttcaat ggcaccgttc ttgctaagtt gacaatggag
      421 ctttggaatt cataatatga acaaataatg attttgccac tatcgcgaca tccttatttg
      481 aaattggttc gtattcgtat taaaggttgt atgtgaagcg ttatcatgtt gagcaaaaac
      541 caatccttcg taacatgcag agcaccatcg ttgaatcgtc cattcgttgt ccggaaaact
      601 gggagaaagg aaactgggag tttattcgtg aatattggag tgacagatat aattatctga
      661 tttacaaacg ggtaatggat gttgtgacat cgcttgtggt cattcttgct atctttccct
      721 ggcttttccc cctcctgttc gtgctgatca agctggattc gaaggggccc gttttcttcc
      781 ggcagaaaag ggtgggtttc cttggtaaga ccttctggtg ctataagttc aggaccatgt
      841 atgtcaatga ctacgccgat acccgccggg cttcccgcaa tgacccccgc gtcacccggg
      901 tcggttggtt cctgcgtaat acctgtctgg atgaagttcc gcaattcttt aatgtccttt
      961 tgggacatat gagcatcgtg gggccccgcc cgcacatgtt gaaggattcc aatgaatttg
     1021 ccgacctggt ctccaattac cagtttcgca acctggcccg tcccggcatc acgggtcttt
     1081 cgcaggtgag gggatatcgt ggtcctgcca cgagtttcga aagtatcttc cgccgctacc
     1141 agtgggattc ctattatgta cgcaacgtaa gttttaccct cgatctcaag atcatggtga
     1201 gtacggccct tctgatgatg cgatccgttt tttaccggga tcagcccgta cccctcgaca
     1261 acaatcggcc cgtttataat tcggaatccc tggtatcagc caaaaagatt gcataggtgc
     1321 tacataatta ctaaagttgt atattcgggg gatgaggatc aaccgacgca ccgctatcca
     1381 ccaattggcc cttctctccg caggcgcggt cctgttgccc tctcggttcc atgagccggc
     1441 ggcagggaaa tccatccccg cctttttctc ctcacatcta cgggctgctt cgtaatccct
     1501 taactctttg tcatgggcga ctcaccaaga aaatttatgg gcatttccgg caacaagggc
     1561 cgcctggcca attttccgga tggggaattc ctgccccctc tcgccgccgc aggcgacggg
     1621 gcttgtacga cctccaccgc aacctcaaac ccgtctctta cgtatagggc attgacggcc
     1681 cgcgcggttg accatgccgg gtcgctgttt ggccacacct gaaatacaaa tcgaatacca
     1741 tgaagggctt cctattgtcg atcgtcgctt ttcttctcat cttcctggtg ctgaactttg
     1801 gctattacca ggaatggttc cgcagcaaac ccgtgcaata ctggtcggac ttcctgaagg
     1861 agaagggcga tacctccagc gaattcgcca tcaagatgca gcgctatggc tttgggtata
     1921 ccgtcagcat ccgggtacag gatgccatgc agaaaaagaa ggtggcccat cctgttatcc
     1981 tcttcgaacc caacagctac taccgcgact ccctgcatat ctatcccaat atccgcgctc
     2041 ccgaacccgc cgtgttctat tattataccg ggatggaagg ggtctggacc aattcccccg
     2101 atgtgaacag ggcgaatttc ctgctccgca tcaccaagaa aggagccggg ctcgatacca
     2161 tcaaatcacc tcaacagctc caggaaatac tggcacgcta taaaaaatat cccccaatcc
     2221 tctaatccga tgcagctact cgcctttttt ttgcttctcc tctgttacct gttgacggga
     2281 tatggccttc tttatctttt taaaatgcgg ctgaaggccg cttatacggt gaccctttcc
     2341 ctgttgctgg ggcttgcggt agcgtccttt ctacccttcc tgttacagct ctgttatgtc
     2401 gagatcacac ccgcgacggt gtttgggatc ctggcgctcg cctgtttggt gatcaatctt
     2461 ccgctgctcg accactaccg gaaagagggg ttcgccgcct ttcggcggtc gctgcccagg
     2521 gcgggcttcc aggtgcgtcc ctatgagatc cctttctggg tgatcatcgg tttcttcatc
     2581 ttcgtcagcg tctggcgctg ttactatctt cctcccacca gccgcgatgc gctgtcggga
     2641 ccggaagcca tcgccgaagt ggctgtacgc gagcataccc tgatcaactc cttctttggg
     2701 atcgatctct ggaccaccaa taaccagttc aagtcgccct tcctgatctc cctgcaggtg
     2761 atctacaaaa tggccggttt cccttttggc caggtctggc tcagcatcgt tttcatcagc
     2821 atcaccgttt tcatctacca tgccctgaga gaaaagatcc accccctgct ggccggtctg
     2881 ctgctgctgc tttttaccat gtcgccggaa atgtatgcct acacctttat gatcctgtat
     2941 gattacagca atatggtgtt cttttttctc ggcatctttt ttctcttccg ctacctgcgg
     3001 gatgggggaa cgaaccagtt gtatttcgcg ggcctgctga tgggcatcgc cacctatatc
     3061 cggagcgaga ccctggccct tgccctgctc tttcttcccc tcctcctgct ggtgcagtgg
     3121 cggaagaaaa tgccggtaaa gaacattgcg ctgggcaacg ggctctttct cctgccctcc
     3181 ctgctcgggt attatctccc ctcccaactc tatataaaat attacctgcc cgtgcactat
     3241 gatatcggcg ggctcgtcaa caaccatcta tcttccctgc agccgctctt ccagcggtat
     3301 ggagatatcg tgacgcgtct cctcgccggg gagttcgcca ttcatctctg gggctatttc
     3361 ttctatttcg ccgcccttct ctttctcgcc gagctcgtct ttgtccggcg gttcagcagg
     3421 gaggcccgca actggctcta tgccgtcctg gtactctacc ttgggctggg cgtccttggc
     3481 tggctgctgc cgatgataaa cctcaacgag acgacaaaac gggcgatgtt caaaatgctg
     3541 ccgctggtgt tgttctacct ggcgaataac gaactgctga tccggctttc gaacgtcatc
     3601 agccgatggg aggaggcccc gttcaggggg agtctcgccg gcgctaccgc cgggagaccg
     3661 gcgccggcat ctgccgcacc gccaaaatcc gtccttacgc ggagcggcaa gaccaagccg
     3721 gcggcgccac ccgcaaaaca gcggaataat ccgcccgccg ggccttcgaa gaagaagaag
     3781 aaataagacg gccgccgccg atccgcaggg cccggcctca ccggagaatg cgaggttcac
     3841 cgaaatacgc gaaaagtgcc attttatgct ggaatagcac aatgacccgc tggcgaaaca
     3901 cggatacctt tatcgggtaa tcagaagccc tgtcatttgc agggtagtta aagccccgtc
     3961 atatgtgtgc cgataaacat caccacgaac aagcgccgcg caggcccaac ctgatcctga
     4021 gtatcctgcg caacagatgc tcccgctgcc gcgaggggcg cctttttatc gatgcaaacg
     4081 cctatcacct gaaacggttt atgaagatga acgagcactg ccccgtctgc ggtcagccat
     4141 tcgagataga accaggtttc tggtatggca cgggcttcgt cagctatgcg cttgccgtcg
     4201 cggtttctgt agccagcttt attgcctgga aggtactgat cgggatgtcc ctgcaggaca
     4261 accggctgtt ctggtggatg ggggtcaatg gtctgctcct ggtgttgtta cagccgcttc
     4321 ttatgcggct ctcccgggcg atctggctca acttctttgt gtggtatgac ccggaatggc
     4381 gttccaggat tgtccggctg gggcgtggaa cttttttgtg aaacgttgtt gcaaattttt
     4441 tttccgcaga taaaaatttt ggttagcttc atcggctaaa ccttattgat atgttggtga
     4501 agacgtttgg aagtgcggtg tatggagttg aagcaatcac catcaccgtc gaagtaaatg
     4561 tgacggaagg cctgcacacg tttatcgtcg ggcttgccga cagtgcggta aaagaaagca
     4621 tccagcgggt agagagtgcg ataaagagca acggttttta tatgccccgg acgaagatca
     4681 tcgtcaacct ggccccggca ggcatccgga aatacggttc ggctttcgat cttcctattg
     4741 ccatcggaat tctcgcggcc agtgaacaga tccccgatgc cgaactgttg aaggactatg
     4801 tcatcatggg agaactgggg ctggacggaa gcatccatcc gatcaagggc gccctgccga
     4861 tcgccgtaca ggcgcgtaaa gagcagttca ggggattgat cgttccacaa caaaatgcat
     4921 ccgaagcggc aatcgtcaat caattgcagg tatactcctt tggacacctc agagaagtga
     4981 tcgggttctt cagggatagt tcttccacaa aggctgtagt tgtcgatgcg cgtgcatcct
     5041 tcttctctca gtcgccgttg ccggacctgg atttcagcga ggtgaaaggc caggaaaaca
     5101 tcaaacgggc gttggaaatc gccgcggcgg gtggtcataa tgccattatg gtcggcccgc
     5161 ctggagccgg gaagacgatg ttggcgaaac gactgccggg catccttccg cccctcagtc
     5221 tccaggaggc cctggaaacg acaaagatcc atagcgtagc gggtaaattg ccggtgcatg
     5281 cgaccctgat cgcgcagcgt cctttccgat cgccccatca taccatttcc ggtatggccc
     5341 tcgtcggggg cggcactatc ccgcagcccg gggagatctc actcgcgcat aacggtgtct
     5401 tgttcctgga tgaattaccg gaattcagcc ggagcacgct ggaaatgatg cggcagccga
     5461 tggaggaccg caaagttttg atcagccgcg ccgccctctc cgtggaattc ccttcctcct
     5521 ttattcttct tgcttcgatg aatccctgtc cctgcgggta ttacaaccac ccggataagg
     5581 aatgcacctg tccgccgggc gcggtatcga gatatcttaa taagatctcc ggtccgttgc
     5641 tggaccgcat cgatctgcat gtggaggtag tgccgctatc ctatgcgcaa ttatccgacc
     5701 gcaccagcgg cgaaagctcc cagcagatcc gtcaacgggt gatcgcggca agagagatac
     5761 aacgccggcg atatgaaggg atatcacccc aggcgatggg gataacgacg tcggctcaca
     5821 gcggcatcta ttgtaatgcg cagatcggcg gccggctttt gcggcattat tgccggatcg
     5881 atacgcatgg ggaa
//
LOCUS       DATGFG010000692         3609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1510254, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000692 DATGFG010000000
VERSION     DATGFG010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510254"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2204
                     /locus_tag="VKQ52_12935"
     CDS             <1..2204
                     /locus_tag="VKQ52_12935"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HLZ88149.1"
                     /translation="DFTLKNLPEGPCILQISVVGYQTVTRMIPAGHRTAGVPFNTGTI
                     RLTPAATQMQVVAVVARRPAFRTEIDKKVFNVDQSLASKGGTAQDALRQVPTLNVDAT
                     GNVSLRNGSPTILLDGKQTQLTLDQIPADQIESIEVMPNPSAKYDAQGNHGIVNIVLK
                     RNRKPGMNGSFTGVGSTLGETYGFANLSVYKHKWNFSFNGMAHGHRSVSNTTTTLNNI
                     PTGTTVIQHGHSVTIGPFGSVRAGADYNMDAHNTFSLYGNVGLGYHPSGGTQTTDFLD
                     KGGLTDSTGMRSSYDANHFVFTHSNFNYAHTFRKASEKLTASASAETYNGHGHGNYAM
                     QYLGKGGSTPGNPYLQQYQGFGKGHNVTLQSDYTDPLLEGRAKFEAGIKTILHGSRSF
                     QDFKDGLPQGFVTDTNASYNYSYNDNTYAAYASFNQELGKFSYLAGMRFERYNYTGHL
                     LDENSSFGYHQTGLYPSVYLTEKFDENNDLHLNYSRRVNRPQWWQITPQTNYGNPQNP
                     QMGNPNIRPENTNLLELAYNTQLSSIGFNTTLYVKNTLDPIMAYNKPLSSDTLLSTFE
                     NANYSNTYGAEIIARIPIAKWWNATTNFNLFETDINADNLTQGLSNTGVSWFAKVNSD
                     MKLFNTYTLQLTGSYNAGNVIAQGKVLPYGGMDAAVKREFLKHNAGTLVLSLSDVFNS
                     QRSRVDTYSQGVFFQDAINKPETRVFKVSFMYSFGKDLSGERHKATLESNG"
     gene            complement(2182..3336)
                     /locus_tag="VKQ52_12940"
     CDS             complement(2182..3336)
                     /locus_tag="VKQ52_12940"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HLZ88150.1"
                     /translation="MHFQLSEEHLMIQKAARDFARTECLPGVIERDEHQKFPKEQVLK
                     LAELGFLGMMVDPAYGGSGMDTVSYVLAMEEISKIDASVSVCMSVNNSLVCWGLDTYG
                     THAQKTQYLTPLAQGRKDGELYIGAFLLSEPEAGSDATSQRTTAEEKDDHYLLNGTKN
                     WITNGNSASVYLVIAQTDVSKGSRGINAFIVEKSSPGVVVGAKENKLGIRGSDTHSIL
                     FNDVRVPKENRIGEDGFGFAFAMKVLAGGRIGIASQALGIASGAYELALEYSKQRKAF
                     GKEIAQHQAIQFKLADMATRIEAARLLCMRAAWDKDQRNDYTLSSSMAKVFASETAMW
                     TTTEAVQVHGGYGYVKEYHVERLMRDAKITQIYEGTSEVQRIVISRSILK"
BASE COUNT          841 a         1079 c          931 g          758 t
ORIGIN      
        1 gggattttac gctgaagaat ctaccggaag gcccctgtat cctgcagatc agtgtcgtcg
       61 gttatcagac ggttacccgc atgatcccgg ctggtcatcg cacggcaggc gttccgttca
      121 atacgggaac catccgcctg acgccggccg ccacgcagat gcaggtagtg gcggtagtag
      181 cccggagacc cgccttcagg accgagatcg ataaaaaagt cttcaatgta gaccagagcc
      241 tggccagcaa gggaggtacg gcccaggatg ccctgcggca ggtgcccacg ctcaacgtcg
      301 acgctacagg caatgtctcc ctgcgaaacg ggtcgccgac cattcttctc gacggcaagc
      361 agacgcagct gaccctggat cagatacccg ccgaccagat cgaaagcatc gaggtcatgc
      421 ccaacccctc cgccaaatac gatgctcagg gcaaccatgg catcgtgaat atcgtgctga
      481 aacgaaaccg caaaccgggg atgaacggtt ccttcaccgg cgtcggaagc accctgggcg
      541 agacctatgg cttcgccaac ctgagcgtct acaaacacaa gtggaatttc agcttcaacg
      601 ggatggccca tggccatcgc agcgtctcca ataccaccac caccctgaat aatatcccta
      661 ccggaacgac ggtgatccag catggccatt cagtgaccat cggtcccttc gggagtgtgc
      721 gggcaggcgc cgactataac atggacgccc acaatacctt tagcctctac ggcaatgtcg
      781 gccttggtta tcacccgtcc ggtggcaccc agaccacgga cttcctggac aagggcggct
      841 tgacggattc cacgggcatg cggtcctctt atgacgccaa ccactttgtc ttcactcact
      901 ccaacttcaa ttacgcacat acgttcagga aggcaagcga gaagctgacc gccagcgcct
      961 ccgcggaaac ctacaacgga catggccatg gcaactatgc gatgcaatac cttggtaaag
     1021 gaggttccac cccgggcaac ccttacctgc agcaatacca gggttttggc aagggacata
     1081 atgtcaccct ccagtccgat tacaccgatc ccctgctgga aggaagggcc aagttcgaag
     1141 ccggcataaa gaccatcctc cacggcagcc gcagcttcca ggatttcaag gatggcctgc
     1201 cacagggttt tgtgaccgac acgaacgcaa gctacaatta cagctataac gacaatacct
     1261 atgcggccta tgcgagcttc aaccaggagc tgggtaagtt cagctacctc gccggaatgc
     1321 gtttcgaacg atacaactat acgggtcacc tcctggacga gaacagcagc tttggctatc
     1381 accagacggg actgtatccc agcgtatacc tcaccgagaa attcgatgag aacaacgacc
     1441 tgcacctcaa ctattcccgc cgcgtcaacc gcccgcagtg gtggcagatc acgccgcaga
     1501 cgaactacgg caacccgcag aacccccaga tgggcaatcc caatatccgg ccggagaata
     1561 cgaacctcct ggagctggcc tataacaccc agctgtcttc catcgggttc aacaccaccc
     1621 tgtacgtaaa gaacaccctg gacccgatca tggcctacaa caagcccctg tccagcgata
     1681 ccctgctcag caccttcgag aatgccaatt acagcaatac ctacggtgcg gagatcatcg
     1741 cccggatccc gatcgcaaaa tggtggaacg ccacaacaaa tttcaatctc ttcgagaccg
     1801 atatcaatgc ggataacctg acccagggcc tgtccaacac gggggtcagc tggttcgcca
     1861 aggtcaattc ggatatgaag ctgttcaata catatacctt gcagctcacg ggcagttata
     1921 acgccgggaa tgtgatcgca caggggaagg tccttcccta tgggggtatg gatgccgccg
     1981 tcaagcgcga gttcctgaag cacaatgcgg gtacgctcgt cctcagcctc tcggatgttt
     2041 tcaattcgca acgctcgcgg gtggacacgt actcgcaggg ggtcttcttc caggatgcca
     2101 tcaacaaacc ggagacccgg gtcttcaagg tcagctttat gtattccttt ggaaaagacc
     2161 tgagcggcga acgccacaag gctactttag aatcgaacgg ctgatcacga tacgctgcac
     2221 ttcgctggtg ccctcataga tctgggtgat cttggcatcc cgcatcagcc gctccacgtg
     2281 atactccttg acatacccat agccgccatg cacctgcacg gcctccgtgg tagtccacat
     2341 cgccgtctcc gaggcaaaga ccttggccat ggaggaactg agcgtataat cgttgcgctg
     2401 gtccttgtcc caggcggcgc gcatacaaag cagccttgcc gcctcgatgc gggtggccat
     2461 atccgccagt ttgaactgga tggcctggtg ctgggcgatc tcctttccaa acgcctttcg
     2521 ctgtttcgag tactccagcg ccaattcgta ggcgccgctg gcgatgccca gggcctggga
     2581 agcgataccg atacgcccgc cggccagcac cttcatcgca aaagcaaacc cgaaaccatc
     2641 ctccccgatg cggttctcct tcggaaccct gacatcattg aataagatac tgtgggtgtc
     2701 gctgccgcgg atacccaatt tattttcctt cgccccgacg acgacgccgg gagaactctt
     2761 ttcgacgata aaggcgttga tgccgcgact gcccttggaa acatccgtct gggcgatgac
     2821 gagatatacg gaggcggaat tgccgttggt gatccagttc ttcgtgccgt taagcaggta
     2881 atgatcgtct ttttcttcgg cggtggtgcg ttgcgaagtg gcgtcgctgc cggcttcggg
     2941 ctcgctcagc aggaatgcgc cgatatagag ctcgccgtcc ttccttccct gggccagcgg
     3001 ggtcaggtat tgcgtcttct gggcgtgcgt gccgtaggtg tccagtcccc agcacacaag
     3061 actgttattg acgctcatac acacgctgac gctggcatcg atcttactga tctcctccat
     3121 tgccaggaca tagctcaccg tatccatccc cgaaccgcca taggcagggt ctaccatcat
     3181 ccccaaaaag cccagttcag ccagtttgag aacctgttcc tttggaaatt tctggtgttc
     3241 atccctttct attacccccg ggagacattc ggtgcgggca aaatcccggg cggctttctg
     3301 gatcataaga tgttcttccg acaattggaa atgcatatgg gcaacaattt tcccaaaaat
     3361 agtagaaagt tgtttagtaa actaacacta gttagttttt ttgtacgcgg acttgctttt
     3421 tgtggagaag gtaacagagg aggcggcggg tggtctatcc cagtatctta tctgccttac
     3481 ggtggagcag ggaaatggca gtcttgaaaa taacgataaa aagacctgct tcttcttcgc
     3541 tgaaattgct ggcatcttcg tcatcgccat cgctatagat ggtggctgcg gcaaattgca
     3601 gcaggtcgg
//
LOCUS       DATGFG010000693         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_821240, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000693 DATGFG010000000
VERSION     DATGFG010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821240"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VKQ52_12945"
     CDS             <1..600
                     /locus_tag="VKQ52_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88151.1"
                     /translation="MMQGPVDFGTAAGLRNRLGISEMGGKTGTTNDNADAWFMGYTPQ
                     LMAGTWIGCDDRFIHLEGGLGYGAQAARPMWEYFFAKVLADKTLGIERQAKFIQPENM
                     RREMMYDYMNIEKTAPPGAEGANQGNGNANKYLDTSPPTVPVDSKLSPDEQKILQEAN
                     RSSKEGSVKITVTDADKKPEQPKKKGGFFKRLFGKKDNN"
     gene            complement(660..1133)
                     /locus_tag="VKQ52_12950"
     CDS             complement(660..1133)
                     /locus_tag="VKQ52_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HLZ88152.1"
                     /translation="MNASIGIAPANLQAVAVELSKLLADEFLLYTKTRNAHWNVEGSD
                     FHSMHIFFEQQYEQLDEVMDGVAERIRQLGHYAPATLQSFLSLTHLTEKLGERNDSLG
                     FIRELLTDHENIIIFLRENIGKFANDYGDAGTSDYITGLMEGHEKMAWFLRAHLK"
     gene            1362..1814
                     /locus_tag="VKQ52_12955"
     CDS             1362..1814
                     /locus_tag="VKQ52_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88153.1"
                     /translation="MKHLSIAVILCLTVIGNTFANPRSGKDELNFAGANNFLEKFPKA
                     TNISYKVKGQFTEVNFIWNGLQLEAFYDRDGNPIATTRSVDQKSLPVNVMLGLKKEYA
                     DGVVICAIEYTDLNDGLSYYVTLASPKTTYLLHVSTSGDISVFRKMKH"
     gene            complement(1811..2278)
                     /locus_tag="VKQ52_12960"
     CDS             complement(1811..2278)
                     /locus_tag="VKQ52_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HLZ88154.1"
                     /translation="MKTRLLAYLLLPVAVYGRDEKPVRDSVPNSPKGHHKPAPVKVQV
                     GVASYYSNKFEGRKTYTDEIFTQEKLTGASNTLPMHTWVRVTNLHNNKSVIVRINDKM
                     HPRNKRLIDLSLAAATRLGYAGRGLTRVRIDVLGKKRPPDAEDDMAIRQPGQD"
     gene            complement(2380..>3049)
                     /locus_tag="VKQ52_12965"
     CDS             complement(2380..>3049)
                     /locus_tag="VKQ52_12965"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLZ88155.1"
                     /translation="HDADTIALESRVIVGAPKTRTPLLTSEISNFVTYPQWTVPTSII
                     MKEMLPAIKRNVDYLRKENLMVVDDNDSVRDPMTINWKRLNKNNFPYQLKQRQGDDNS
                     LGVMKFNFRNKYDVYLHDTNVRWMFDKNFRALSHGCVRVKEWRKLSAFLIRNDTMRYH
                     PDTLKNWISRQEKHTVYGFAHVPIFLRYFTAEGKKGKIQFYDDIYGEDRALRNKYFAQ
                     KPIY"
BASE COUNT          803 a          865 c          706 g          675 t
ORIGIN      
        1 atgatgcagg gccccgtcga cttcggcacc gccgccggtc tccgcaaccg cctcggcatc
       61 tccgaaatgg gcggcaaaac gggcaccacc aacgacaacg ccgacgcctg gttcatgggc
      121 tacaccccac agctgatggc aggcacctgg ataggatgtg acgaccggtt catccacctc
      181 gagggcggcc tcggctacgg cgcgcaggcc gcacgcccca tgtgggaata tttcttcgcc
      241 aaagtactgg ccgacaaaac cctgggcatc gaacgccagg caaaattcat ccagccggag
      301 aatatgcgca gggagatgat gtatgattat atgaatatcg agaagaccgc gccgccggga
      361 gccgaaggcg ccaatcaagg gaatggcaat gccaacaaat acctcgacac cagcccgccg
      421 accgtgccgg tggactccaa actgtcgccc gacgaacaaa agatcctgca ggaagccaat
      481 agaagtagta aggaaggctc ggtaaagatc accgtcaccg acgccgataa aaaaccggaa
      541 caaccaaaaa agaaaggcgg cttcttcaaa cgcctcttcg gcaaaaaaga caacaactaa
      601 aaaaaacagg ccgtctcaaa gcaaaagtag ccggcctgtt ttttcacaat gtccttcatt
      661 tacttcagat gcgcccgtaa gaaccaggcc atcttctcat gcccctccat caaccccgta
      721 atgtaatcac tcgtacccgc atcaccatag tcattggcaa acttgccgat attctcccgc
      781 aggaaaataa tgatgttctc gtggtcggtc aacaactccc tgataaaacc aaggctgtca
      841 ttccgctcac ccaacttctc cgtcagatgc gtaagagaaa gaaaactctg cagggtagcc
      901 ggcgcataat ggcccaattg acggatcctc tctgcaacac cgtccatcac ctcatcgagc
      961 tgctcgtact gctgctcgaa gaagatatgc atggaatgaa aatcggagcc ctcaacattc
     1021 cagtgcgcat tccgggtctt ggtgtacaaa aggaactcgt ctgccagtaa cttgctcaat
     1081 tcgactgcta cggcctgaag attcgcagga gcgatgccaa tgcttgcgtt catagtttta
     1141 ttttttagat ttttgaatgc tttccgatgg gcctccggcc catttgtaat acaaccgaac
     1201 aacaaatcgt ccggaaccgg acactctcat ttgtgacaaa aaagttaacg ccactcacaa
     1261 tcaacagctt acgacatgta acatctttgg cattgcgttg gtcctatagg atacggctag
     1321 ctacagaaaa aatcaggaat aaacagtaaa acaaaacaat tatgaaacac ctatccattg
     1381 ccgttatact atgcttaacc gttatcggca acacattcgc caatccccgt tccggcaagg
     1441 acgaactcaa tttcgccggc gcgaataact tccttgaaaa gttccccaag gccaccaaca
     1501 tcagttacaa agttaaggga caattcacag aagtcaactt tatctggaac ggcctgcaat
     1561 tagaggcatt ttacgaccgg gacggcaatc ccatcgccac tacccgtagc gtcgaccaga
     1621 aaagcctccc cgtaaatgtt atgctcggtc tgaaaaaaga atatgcagat ggcgtcgtca
     1681 tctgcgccat cgaatatacc gacctaaacg acgggctcag ctattatgtc acgctggcca
     1741 gtccaaaaac gacatatctg ctgcatgtgt ctacaagcgg ggatatcagt gttttcagga
     1801 aaatgaaaca ttaatcctgc ccgggctgcc ggatagccat atcgtcctcg gcatccggcg
     1861 gccttttttt acccagcacg tcgatcctca cccgggtaag cccccggccg gcatagccca
     1921 atcgcgtggc agccgccagg ctaagatcga taagccgttt gtttcggggg tgcatcttgt
     1981 cattgatccg gacaatgacg gatttgttgt tgtggagatt ggtcacccgg acccaggtat
     2041 gcatcggaag cgtattactg gcccccgtca gtttctcctg cgtaaagatc tcatcggtat
     2101 aggtcttccg cccttcaaat ttgttggaat agtaagaggc aaccccaacc tgcactttca
     2161 cgggggccgg cttgtgatgc cccttggggg aattgggaac agaatcccgt acgggtttct
     2221 cgtcgcggcc atagacagcc acaggcagga ggagataggc gagtaatcgg gttttcattt
     2281 atttggattt agccggccgg aggccggctg cccgaagggc cataataagc cctacggagg
     2341 tgccgcaggc gccgatgccg accagcgacc tcaatatact taataaattg gtttctgagc
     2401 aaaatatttg ttcctcagcg cccgatcctc cccatagata tcatcataga actggatctt
     2461 ccccttcttc ccctccgccg taaaataccg caaaaagatc ggcacatgcg caaacccata
     2521 caccgtgtgc ttctcctgcc gcgagatcca gttcttcagc gtatccgggt gataccgcat
     2581 cgtgtcatta cggatcagga aggccgataa tttccgccat tcctttaccc gcacacagcc
     2641 atgactcaac gcccggaaat tcttgtcgaa catccaacgg acattggtat cgtgcagata
     2701 aacatcatat ttattcctga aattgaactt catcacccca agggagttat cgtccccctg
     2761 gcgttgcttc aattggtaag gaaaattatt cttgttgagc cgcttccagt tgatcgtcat
     2821 cgggtcgcgt acactatcgt tatcatccac caccatcagg ttttccttcc gcaggtaatc
     2881 aacattccgt ttaatggccg gcagcatctc cttcatgatg atactggtcg gcaccgtcca
     2941 ctgcggatag gtcacaaaat tggagatctc gctggtcaag agaggggtcc gggtcttcgg
     3001 cgcaccgacg atcacacgcg actccaatgc gatcgtatcg gcatcatgg
//
LOCUS       DATGFG010000694         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1400799, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000694 DATGFG010000000
VERSION     DATGFG010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400799"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VKQ52_12970"
     CDS             complement(<1..643)
                     /locus_tag="VKQ52_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88156.1"
                     /translation="MKTSPLIAIILVVTVYACQKALPETDPNLRALVGAPGLLPPKDS
                     GSAKHDSSASKPADTTKPSPKSDTATVPPPPFPQQTINNNNTTGTTAPSCPVSPIYGD
                     TLVFPQPTAGDDVILPVNNPGVGKYFSWPVGMVLDQNTGAINLTQSQTGMKYIMGFVK
                     SGSTDTCLSTLIIGGAAYFDSVYVFADGQTTASPYFEANPYLPSVCANGGCTFD"
     gene            971..1360
                     /locus_tag="VKQ52_12975"
     CDS             971..1360
                     /locus_tag="VKQ52_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88157.1"
                     /translation="MKQFIAIIALFFAFSFTTKAQSVYYATKSYQGTWSAYSQKYIWG
                     SPYNVSIRFTIQGNIVLVGDRAGSTYTTGTEIIDRYDSDGGHEYGWNATDETGVNCTF
                     KLTNYTDGSKLIEVLYSNYAYMYVIPS"
     gene            complement(1480..>2046)
                     /locus_tag="VKQ52_12980"
     CDS             complement(1480..>2046)
                     /locus_tag="VKQ52_12980"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLZ88158.1"
                     /translation="DGIEQFKSTNMKSISIGRDPECQLPIQDTEVSWKHCVLYERSDG
                     RAELEDLGSTNGTFVNGQKKSSVTVPLYPGDVVQVGHAIVQWEQLLGREQGVQRGVSR
                     ETVRANPMEKPWTRPAGAPVSYRDGGGGRDAARDVHLHIDQKGRQGGVEKFETGAKGF
                     LQIAKAAFYIALAILVFWLVFKVFHVFG"
BASE COUNT          501 a          678 c          432 g          435 t
ORIGIN      
        1 catcgaacgt acagccgccg ttggcgcata cagacggcag atagggattc gcctcgaaat
       61 acggggaagc cgtcgtctgc ccatccgcaa atacatacac actgtcgaaa taggctgccc
      121 caccgatgat cagcgtgctc aaacaggtat ccgtcgaccc gctcttcaca aaacccatga
      181 tatacttcat acccgtctgg ctctgagtca ggttgatagc acccgtattc tgatccagca
      241 ccatgcctac cggccaactg aaatatttgc ccacacccgg gttgttcacc ggcaggatga
      301 catcatcccc cgccgtcggc tgcgggaaaa cgagcgtatc accataaatg ggggaaacag
      361 gacaacttgg agcagtcgtc ccggtagtat tgttattgtt gatggtctgt tgagggaaag
      421 gcgggggcgg aacggtcgca gtatccgact tgggtgaagg cttcgtcgta tccgcaggct
      481 tcgacgccga tgaatcatgc ttggccgaac ccgagtcctt cgggggcagc agccccggcg
      541 cccctaccag cgcccgcaaa ttcgggtcgg tctccgggag ggccttttga caggcatata
      601 ccgtgaccac aaggataatg gcaatcagcg gactagtttt cataagtagg tatttagata
      661 ggggttaacg cttaacaaaa tacaaattat tcccggtttc tctaagcttt ggtaaccgcc
      721 gcttgcctat tcgtaaccgc caaatccaag aatatttaca atattttgat aatcaataaa
      781 ttaataaacc cccttggagc aaagcccccc caccgcgcaa aaccctgaat actcccgcat
      841 ccttcccctt caggcgaaac caaaaatcct ccctcccgac aaataccagt tagcgactgg
      901 tggctatacc gccaacggct ttttcatgga tttactttgt atcatcaatt aacgccaaaa
      961 aaacaaaacc atgaaacagt tcatcgccat catcgccctc ttcttcgcct tctcttttac
     1021 gacaaaggcc cagtctgtct actatgctac caaatcctac cagggaacct ggagcgccta
     1081 ttcccaaaaa tacatctggg gctcccccta caacgtcagc atccgcttca ctatccaggg
     1141 taacatcgtc ctcgtaggcg accgcgccgg ctctacctac accaccggca ccgagatcat
     1201 cgatcgctac gacagcgacg gcggccacga atacggctgg aacgccaccg acgaaaccgg
     1261 tgtcaactgt accttcaaac tgaccaacta taccgacggc tccaaactca tcgaggtcct
     1321 gtacagcaac tacgcctaca tgtacgtgat ccccagctaa acaatgccgt ccccaggcgc
     1381 ccttccccca ggccgccact ccacctcctg cagctttgac acctcgtcaa agctttttgc
     1441 attacttatc cagatagatg actcatccca atacatgact tatccaaata catgaaacac
     1501 cttgaacaca agccaaaaaa caaggatcgc cagggcgata taaaaggccg ctttcgcgat
     1561 ctgcaaaaac cccttcgccc ccgtctcgaa cttctctacc ccaccctgac gccccttctg
     1621 atcgatatgc agatgcacat cccgcgccgc atcccggccg ccgccaccat cccggtacga
     1681 gaccggcgct cccgcgggtc gcgtccaggg cttctccatc gggtttgccc gcaccgtctc
     1741 ccgtgacacc ccccgctgca ccccctgctc ccggcccagc aattgctccc actgcacgat
     1801 cgcatggccc acctgtacca catcccccgg atacaacggc accgtgaccg aactcttctt
     1861 ctgtccattc acaaaggttc cattggtgga cccaagatcc tccaactccg cccggccatc
     1921 gctccgctca tacagcacac agtgcttcca ggaaacctcg gtatcctgga tcggaagctg
     1981 acactccggg tcccgcccga tactgatcga cttcatattg gttgatttaa attgttctat
     2041 tccgtc
//
LOCUS       DATGFG010000695         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_459049, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000695 DATGFG010000000
VERSION     DATGFG010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459049"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /gene="ligD"
                     /locus_tag="VKQ52_12985"
     CDS             complement(<1..1436)
                     /gene="ligD"
                     /locus_tag="VKQ52_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HLZ88159.1"
                     /translation="MALKEYKQKRSFSATPEPQGGKPLGRVLQFVVQKHAASRLHYDF
                     RLEMDGVLKSWAVPKGPSLNPADKRLAMMVEDHPFDYKDFEGIIPTGNYGAGTVMVWD
                     EGTYEALEETNGTKAAQEKALLKELREGSLKFRLNGHKLKGEFALVRTKGRGENSWLL
                     IKHRDRYASEKPVTDKDKSVVSKKTLEQIAKNPKAKEWISNKDGAAAPKTMRKGKTVD
                     KGRAAEGRKVALPGKGKKTAMPAKGKKAAMPADIAPMLATLVDKPFDEEGWSYEIKWD
                     GYRALAYLKKGKVELRSRNNKSFEKYYPVYDALKEWPVHAVLDGEIVVLDAKGQSNFS
                     GLQNWRSEADGELIYYVFDLLWLEGKDLMGLPLAERRELLRSIVPEEGPIRYSDSFET
                     SGTKFFATAQKMGLEGIIAKRTDSLYTPGVRTKEWLKIKTVSRQEVVIGGYTRNEGTS
                     KLFSSLLVGVFEKGKLHYTGKIGTGFTE"
     gene            1505..1735
                     /locus_tag="VKQ52_12990"
     CDS             1505..1735
                     /locus_tag="VKQ52_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88160.1"
                     /translation="MTTGKSKKTEKDPIRDAEEQAEKDILNDPELSDSDPTDDLDEGE
                     LAERDNSDEEAFDELEKKRPSPKHHSGKGNEK"
     gene            complement(1708..3339)
                     /locus_tag="VKQ52_12995"
     CDS             complement(1708..3339)
                     /locus_tag="VKQ52_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLZ88161.1"
                     /translation="MESAAFTDGAAQRELEQKQAMLAAIVDHSEDAIISKTLDGVIMS
                     WNRSAERLFGYTEAEAIGKHISILIPPDKLPEEDMIIDRIRHGQRVPPLQTVRVAKDG
                     HLVPVSIAVSPIRSRDGRIIGASKIARDISEQLRALHELEEHAQTQELLLSATKVISA
                     QLDLQTLLQKVTDIVTQLAGAEYGAFFYNRVDAAGEVYMLHTLSGAPREAFERLGMPR
                     NTAVFQKTFNGEGILRSDDITKDPRYGHNAPHTGMPEGHLPVVSYLAVPVNAPTGQTI
                     GGLFLGHSKPAMFKEKHEQLLSSIAAQAGVAIENAKLYEAIKQLNSRKDEFIGVAGHE
                     LRTPITTIKGYLQLLEDQAAEGLQKEFTEKALRQVNKLNRLITDLLDVSKIQAGRLEY
                     NMMACFLLPLVRESVDTVRQIHTSHRIDTEFPPEDIIVTADGVKIEQVIINFLTNAIK
                     YSPEGSRISLCIKKEERRVVVAVRDWGIGIAPQHQANIFHRYYRVNASEHAIGGLGIG
                     LYISREIVERHGGSIWVESEERKGSVFYFSLPLPE"
     gene            3469..3882
                     /locus_tag="VKQ52_13000"
     CDS             3469..3882
                     /locus_tag="VKQ52_13000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HLZ88162.1"
                     /translation="MPLLEKQLVVKRSTLKGAGKGLFTTRDIPKGIKIVEYKGKVTKW
                     KEVNDRNGLNGYIYYVNRNHVIDAWSTKDALARYANDAKGMCRGEGLNNNCTYRIEGL
                     RVFIYSMRNIPAGSELFVGYGKEYWDIIKENWGVK"
     gene            complement(3884..>4093)
                     /locus_tag="VKQ52_13005"
     CDS             complement(3884..>4093)
                     /locus_tag="VKQ52_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88163.1"
                     /translation="KQRVFPKGLKKRLAVEAASPMGWHKYVTDEGDMLGMTRFGESAP
                     AADLYKEFGFTVANVVARAKALLGR"
BASE COUNT          908 a         1351 c          939 g          895 t
ORIGIN      
        1 tcctcggtaa accccgtccc gatcttgccg gtataatgca atttcccttt ttcaaatacc
       61 ccaaccagca ggctgctgaa cagcttcgac gtcccttcat tgcgggtata cccgccaatc
      121 acgacctcct gccggctcac cgtctttatc ttcagccatt ccttcgtgcg gacccccggc
      181 gtataaagac tatccgtccg cttggcgatg atcccctcca accccatctt ctgtgccgtg
      241 gcaaagaact tcgtcccgct ggtctcaaag ctgtcgctgt atcggatagg tccttcctcc
      301 ggtacgatcg atcgcaacag ctcccgccgc tcggccagcg gcagacccat caggtccttg
      361 ccttccagcc agagcaggtc gaatacataa taaatgagct ccccatcggc ctcactgcgc
      421 cagttctgca gcccgctgaa attggactgc cccttcgcat ccagcacgac gatctctcca
      481 tccagcacgg catggacggg ccattccttc agcgcatcat agacaggata atatttctcg
      541 aaagatttgt tgttacggct acgcagttcc accttcccct ttttcagata ggccagcgcc
      601 cggtacccat cccactttat ctcatacgac catccctcct catcaaatgg cttgtccacc
      661 agcgtcgcca gcatcggcgc gatgtccgcc ggcatggccg ccttctttcc cttggccggc
      721 atcgccgtct ttttcccctt ccccgggaga gccaccttcc gcccttccgc tgccctcccc
      781 ttatccacgg tcttcccctt ccgcatcgtc ttcggcgccg ccgccccatc cttattgctg
      841 atccattcct tcgccttcgg attcttcgct atctgctcca gcgtcttctt cgacacgacc
      901 gacttgtcct tatccgtcac cggcttctcc gacgcatacc ggtcccggtg cttgatcagc
      961 aaccacgaat tctccccccg ccccttcgtc cggacaagcg cgaattcccc ttttagctta
     1021 tgcccgttga gccggaactt cagcgacccc tcccgcaact ccttcagcaa cgccttctcc
     1081 tgcgccgcct tcgtcccatt ggtctcctcc aacgcctcat acgtcccctc atcccagacc
     1141 atcaccgtcc ccgcaccata gttgcccgtc gggatgatcc cttcaaagtc cttataatca
     1201 aacggatgat cctccaccat catcgccagc cgcttgtcgg cgggattgag cgagggcccc
     1261 ttgggaaccg cccagctctt caacacccca tccatctcca gccggaaatc ataatgcagc
     1321 cgcgacgcgg catgtttctg taccacgaac tgcaggaccc tccccagagg ctttcctccc
     1381 tgcggctcgg gagtagcgga aaaactccgc ttttgtttgt attcttttag tgccataact
     1441 tagtagaggc aaacgctttg ccaacctcag gtacaacttt tgtgaaatag acaaaaacag
     1501 gaacatgaca acaggcaaaa gcaaaaagac agaaaaggat ccgatccggg acgcggagga
     1561 acaggcggaa aaagacatcc tcaacgaccc ggagctcagc gacagcgatc ccaccgacga
     1621 cctcgacgaa ggcgaactgg ccgagcgcga taactccgac gaagaagcct tcgatgagct
     1681 cgagaagaaa cgcccctccc caaaacatca ctccggcaag ggcaacgaaa aatagaacac
     1741 cgaccccttc cgctcttcgc tctccaccca gatggaaccg ccatgtcgtt cgacgatctc
     1801 ccgggaaata tacaacccga tccccaaccc gccgatggca tgctccgatg cattgacccg
     1861 gtaatagcgg tgaaagatat ttgcctggtg ctgcggcgca atgccgatgc cccaatccct
     1921 caccgccacc accacccggc gctcctcttt ctttatgcaa agcgagatcc ggctgccctc
     1981 gggggaatat ttgatcgcat tcgtcaggaa attgatgatc acctgctcga tcttgacacc
     2041 gtctgcggta acgatgatat cttccggcgg gaactcggta tcgatccggt gactggtatg
     2101 tatctggcga acggtgtcca cgctctcgcg cacaagcggc aatagaaaac atgccatcat
     2161 attatattcc agccggccgg cctggatctt cgaaacatcc agcagatcgg tgatcagccg
     2221 gttcagtttg ttcacctgcc gcagcgcctt ctccgtaaac tccttttgca aaccttccgc
     2281 ggcctggtct tccagcaact gcaaataacc cttgatcgtc gtgatcggcg tccgcaattc
     2341 atgaccggcc acaccgataa attcatcctt gcggctgttc agttgcttga tcgcctcgta
     2401 cagcttcgca ttctcgatgg cgactcccgc ctgcgccgcg atactgctca ataattgctc
     2461 atgcttttcc ttgaacatcg ccggcttcga atgccccagg aaaaggccgc cgatcgtctg
     2521 ccccgtcgga gcattgacgg gcacggctaa atagcttacc accggcaaat gcccttccgg
     2581 cattccggta tgcggcgcat tatgcccgta tcgcggatct ttggtgatat cgtcgctccg
     2641 caggatccct tccccattaa aggtcttttg aaagacggcc gtattccggg gcatcccaag
     2701 cctttcaaac gcctcccgcg gcgcacccga cagggtatgc agcatatata cttcacccgc
     2761 ggcatcgacc cggttataaa aaaaagcgcc atattccgca ccagccagct gcgtcacgat
     2821 atccgtcact ttttgtagca gcgtttgcag atcgagttgc gccgaaatga ccttggtcgc
     2881 cgaaagcagg agctcctggg tctgggcatg ctcctccagc tcatgcagcg cccgcaactg
     2941 ctccgaaata tccctggcga tcttggaggc gccgatgatc ctgccgtccc tgctccggat
     3001 cggcgaaaca gcgatagaca ccgggacaag atgtccatcc ttggccaccc gcaccgtttg
     3061 aagggggggt acccgctgcc catgccgtat cctgtcgatg atcatatcct cttccggcaa
     3121 cttatccggc gggatcagga tcgagatgtg tttcccgatg gcctccgctt cggtataccc
     3181 gaagagacgc tccgcactgc ggttccagct catgatgact ccgtcgaggg tcttgctgat
     3241 gatggcgtct tcggaatgat cgacaatagc ggccagcatg gcctgcttct gctccaactc
     3301 ccgttgcgcg gcgccatcgg taaatgcggc ggattccata agtgtctttg gggtcgatat
     3361 tgtttaactt gtcggggaaa acagacatta aaactaaaac aaaacagcca aaaaaccttg
     3421 atcgataacc cctatatttg cgacgctcaa acgatcaatc attcttcaat gccgttactg
     3481 gaaaaacagc tcgtcgtcaa acgcagtacc ctgaaaggag ccggaaaagg gctcttcacc
     3541 acaagggaca tccccaaagg aataaaaatt gtagagtaca aaggaaaggt cacgaaatgg
     3601 aaggaggtca acgaccgcaa tggcctcaac ggctatatct actacgtcaa ccgtaaccat
     3661 gtcatcgacg catggtccac aaaggacgcc ctggcccgtt atgccaacga cgcaaaagga
     3721 atgtgccgcg gcgaaggcct taataacaac tgtacctacc gcatcgaagg actccgggtc
     3781 ttcatctact ccatgcgtaa catccccgcg ggtagtgaac tcttcgtcgg ctacggcaag
     3841 gaatactggg acatcataaa ggagaactgg ggggtaaaat aactcacctc cccaggagcg
     3901 ccttggcccg cgcaacaaca ttcgctacgg taaacccgaa ttccttgtac agatcggcag
     3961 ccggcgccga ttcgccaaaa cgtgtcatgc ccagcatatc cccctcgtcc gtgacatatt
     4021 tatgccagcc catcggcgag gcagcctcga cggcaaggcg cttcttcagg cccttcggga
     4081 atacccgctg ttt
//
LOCUS       DATGFG010000696         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_535685, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000696 DATGFG010000000
VERSION     DATGFG010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535685"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VKQ52_13010"
     CDS             complement(<1..445)
                     /locus_tag="VKQ52_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLZ88164.1"
                     /translation="MDKLFAEKIRKPISGYVILVLEVVVVAMAFMFMVGGVTAPGVVL
                     WLLFIFLVRGLLVIPPNTAKVLLLFGKYEGTVRESGFFWMNPFYQRLTVSMRARNFES
                     EQLKVNDKMGNPVLISMVLVWKVRDTYRASFEVDQYENFIKVQTDS"
     gene            553..1608
                     /locus_tag="VKQ52_13015"
     CDS             553..1608
                     /locus_tag="VKQ52_13015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLZ88165.1"
                     /translation="MSQQFRLSVLDQTPIRKDADAPSALQESIRLARLTDRLGYTRYW
                     LSEHHNTTTLAGAAPEILIARLAAETHRMRIGSGGIMLPNHSTLKVAENFRLLEALYP
                     GRIDLGIGRAPGGDRLTAQLLNPTNTFDPQDYIKQLRDLEAFLTDTPGAGNYEGKIRA
                     IPRIETRPELWMLTSSGESAFLAAHFGMALCYAQFINPVGGPEAIRAYRSRFKPSATL
                     GEPKTAVGIFAFCSEDPEKIHETQALIDYRLLGFEKGQYDEIPTVAAALAYDYTPAEW
                     QRVLHNRRRTAIGTPEQLRSAFAGLAADFDVDEFVISTFTEQSADRLHSYELLARLFE
                     LSPGEIPNFRDHLPVHT"
     gene            1605..3026
                     /locus_tag="VKQ52_13020"
     CDS             1605..3026
                     /locus_tag="VKQ52_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HLZ88166.1"
                     /translation="MKPIKRVLFLLSLTITQTLSAQQHPNIIFILADDLGYGEVGYQG
                     QQKIHTPNIDQMVTLGMRFTQFYAGTAVCAPSRASLMTGLHTGHTAIRGNRGYKPEGQ
                     YPLPDSSLTIATVLQNHGYTTGAFGKWGLGYPGSTGTPGKKGFTGFFGYNCQTLAHNY
                     YPDHLWENDTRIDLPNTKDKDSVYSGDLIHQHALRFLRDNRDHPFFLYLAYTLPHAAL
                     QVPHDSIYNQYITQFNEPPKTPRPDDKQPDYAFEPYPHAAFAAMVTRLDHYVGDILRE
                     VQSLGLAGNTLIFFTSDNGPHQEGGGDPAFFHSSGPLRGIKRDLYEGGIREPFLAVWP
                     GKIQPGATSNHTGAFWDLFPTFEQVAGIPATKSIDGLSILPVLLGQPEKAKTHDYLYW
                     EFHENGGRQAVRQGKWKAVRLNVNTVADPPIELYDLEKDPGEQQNVAAQHPDIVRKMK
                     ANMQQAHRSNKDWPLLAGEINPH"
     gene            complement(3023..4036)
                     /locus_tag="VKQ52_13025"
     CDS             complement(3023..4036)
                     /locus_tag="VKQ52_13025"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLZ88167.1"
                     /translation="MNSVNLKQLARELNLSVSTVSRALRDSWEISTPTKQRVFDLAKK
                     MNFQPNPYASSLRKHRSRTIAVVIPEVANNFFALAIKGIETVAREKGYHVLIYLTNED
                     VAEEVAVAGSLQSGRVDGVILSLCGSSQDTRHLLELQQRNIPLVFFDRVSDGVAAPKV
                     MTNDHESGFVATEHLIKAGCRRVAHLTISMNLSISHKRQQGYVDGLRRYEQPWDQDLI
                     VHCGADDAGNYRMIKELFLSEKRPDGVFASVEKLAVQVYHVCRELGLRIPEDVKVISF
                     SNLVTASLLNPSLTTIAQPAYEMGKEAATILFRRLEKKYIVVPEDRVMLPARLEVRDS
                     TMS"
BASE COUNT         1003 a         1572 c          896 g          752 t
ORIGIN      
        1 ccgaatccgt ctgaaccttt ataaaattct catactggtc cacctcgaaa gacgcccggt
       61 acgtgtcccg taccttccat accaggacca tactgatcaa caccgggttc cccatcttgt
      121 cattcacctt caactgctca ctctcgaaat tccgcgctct catagagacc gtcagccgct
      181 ggtagaaagg gttcatccaa aagaacccgc tctcccgcac cgtcccctcg tatttgccaa
      241 atagcaataa caccttggcc gtattcgggg ggataaccaa cagcccccgg accaggaaaa
      301 taaaaagcag ccaaagcacc acgccgggag cggtaacacc ccccaccata aacataaacg
      361 ccatagccac caccaccact tccaaaacaa gaataacata cccactaatg ggtttgcgga
      421 tcttctccgc gaaaagcttg tccataaaga agattttaag gtctgatatc aaaatgatat
      481 cataatgata caaatctacg gtcccggaac cgaaaatgca aaccattcgt cccttttctt
      541 gttaggtagt tcatgtcgca gcaattccgc ctcagcgtcc tcgaccagac cccgatccgc
      601 aaggatgccg acgccccgtc cgccctccag gaatccatcc gcctcgcccg cctgaccgac
      661 cggctcggct acacccgcta ctggctctcc gaacaccata ataccaccac actcgccggc
      721 gcggcacccg agatcctcat cgcccgcctc gccgccgaaa cccaccggat gcgcatcggc
      781 tccggcggca tcatgctccc caaccatagc accctgaaag tcgccgagaa cttccgcctc
      841 ctcgaagccc tctaccccgg ccgcatcgac ctgggcatcg gccgcgcccc cggcggcgac
      901 cgcctcaccg cccaactcct caaccccacc aataccttcg acccccagga ttatataaaa
      961 cagctccgcg atctcgaagc cttcctcacc gacaccccag gagccggtaa ctacgaagga
     1021 aagatccgcg ccatcccccg catcgagacc cggcccgaac tgtggatgct gacctccagc
     1081 ggcgaaagcg ccttcctcgc cgcccacttc gggatggccc tctgctacgc ccagttcatc
     1141 aaccccgtcg gcggccccga agccatccgc gcctaccgca gccgcttcaa accatccgct
     1201 accctcggcg aaccaaaaac agccgtcggc atcttcgcct tctgctccga agacccggaa
     1261 aaaatacacg agacccaggc cctcatcgac tatcgcctcc tcgggttcga aaaaggccag
     1321 tacgacgaga tccccaccgt cgccgccgct ctcgcctacg actatacgcc ggcggaatgg
     1381 caacgcgtcc tccacaaccg ccgccgcacc gccatcggca cccccgaaca actccgctcc
     1441 gcattcgccg ggctcgccgc cgacttcgac gtcgatgagt tcgtcatttc caccttcacc
     1501 gaacaatccg ccgatcgcct ccactcctat gaattattgg cccggctatt tgaactaagc
     1561 ccgggagaaa tccctaactt tagggatcac ctgcctgttc acacatgaag ccgataaaac
     1621 gcgtcctctt tcttctcagc ctcaccatta cccaaaccct ttccgcacag caacacccaa
     1681 acatcatctt catcctcgcc gatgacctcg gctatggcga agtaggctac caggggcagc
     1741 agaagatcca cacccccaat atcgaccaga tggtcacact ggggatgcgt ttcacccaat
     1801 tctacgccgg cacagccgtt tgcgccccgt cacgtgcgtc gctcatgacc ggcctccaca
     1861 ccggccacac cgccatccgc ggcaaccgcg gctataaacc cgaaggccag tatcccctcc
     1921 ccgacagcag cctcaccatc gccaccgtcc tccaaaacca cggctatacc accggcgcat
     1981 tcggcaaatg gggcctcggc taccccggct ctaccggcac acccggcaaa aaaggcttca
     2041 ccggcttctt tggctacaac tgccagaccc tcgcccataa ctactacccc gaccacctct
     2101 gggaaaacga tacccgcatc gacctgccca acacaaaaga caaagactcc gtctactccg
     2161 gcgacctcat ccaccagcac gccctccggt tcctccgcga caaccgcgac caccccttct
     2221 tcctctatct cgcctatacc ctgccccacg cagcactcca ggtaccacac gacagcatct
     2281 acaatcagta tatcacccaa ttcaacgaac ccccaaaaac accccgcccc gacgataaac
     2341 agccggatta cgccttcgaa ccctatcccc acgccgcctt cgccgccatg gtcacccggc
     2401 tcgaccacta cgtcggcgac atcctccgcg aagtacaaag cctcggcctc gccggcaaca
     2461 ccctcatctt cttcaccagc gataacggcc cacaccagga aggcggcggc gacccggctt
     2521 tcttccacag cagcggcccc ctccgcggga tcaaacgcga cctctacgaa gggggcatcc
     2581 gcgaaccctt cctcgccgtc tggcccggga aaatacaacc cggcgccacc agcaaccaca
     2641 ccggcgcctt ctgggatctg ttccccacct tcgagcaggt ggccggcatc cccgccacaa
     2701 aaagcatcga cggcctctcc atcctccccg tgctgctggg acagcccgaa aaagcaaaga
     2761 cccacgacta cctctattgg gaatttcatg agaatggcgg cagacaagcc gtccgccagg
     2821 gaaaatggaa agccgtccgg ctcaacgtca ataccgttgc cgacccgccc atagaattat
     2881 acgatctgga aaaagacccc ggcgaacaac agaacgtcgc cgcccaacac ccggacatcg
     2941 tccgaaaaat gaaagccaat atgcagcaag cccatcgatc caacaaagac tggcccctcc
     3001 tggccggcga gatcaacccc cattaactca tcgtcgagtc ccgcacctcc agcctcgccg
     3061 gcaacatcac ccggtcctcc ggcacaacaa tatatttctt ctccagccgc cggaacagga
     3121 tcgtcgccgc ctccttaccc atctcatagg caggctgcgc aatcgtcgtc agcgacggat
     3181 tcaaaagcga agccgtcacc aggttggaga aactgatcac cttcacatcc tccgggatcc
     3241 gcaaccccaa ctcccggcat acatgatata cctgcaccgc cagcttctcc accgacgcaa
     3301 aaaccccgtc aggtctcttc tccgacagga atagctcttt tatcatccgg taattcccgg
     3361 catcatcggc gccgcaatga acgataaggt cctggtccca gggctgctcg taccgccgca
     3421 acccatccac atacccctgc tgccgcttgt gcgagatact aaggttcatg gagatcgtca
     3481 gatgcgccac ccgccggcaa ccggccttga taagatgttc cgtggcgacg aacccgctct
     3541 catggtcgtt cgtcatcacc ttcggagcgg caaccccatc actcacccgg tcgaagaaca
     3601 ccagcgggat attccgctgc tgcagctcca gcagatgacg cgtgtcctgc gacgacccgc
     3661 aaagcgaaag gataacccca tccacgcgcc cgctctgcag gctgccggca accgccacct
     3721 cctccgccac atcctcattg gtcaggtaga tcaggacatg ataccccttc tcccgggcta
     3781 ccgtctcgat acccttgata gccagggcaa aaaagttgtt ggccacctca ggaataacga
     3841 cggcgatggt tcggctgcga tgcttgcgaa gactgctggc ataaggattg ggctggaaat
     3901 tcatcttctt ggccaggtca aagacccgct gcttggtggg agtactgatc tcccagctgt
     3961 ccctcagcgc ccggctcaca gtggagacag acagattcag ctcacgagcc agttgtttta
     4021 ggttgaccga gttcatatgt agcaggcaat tgtttgcatc cgaaaattac gcaatcccct
     4081 ccccggcccc gattttattt acgaaaacgt tgtagttccg gacggactca tcccggcggg
     4141 ttcttcgcaa ccgtttccgt aaataaagta atgaacgact gccaattatc ctgaaacttt
     4201 tctccaattt gaccccggca ata
//
LOCUS       DATGFG010000697         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_689999, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000697 DATGFG010000000
VERSION     DATGFG010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689999"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VKQ52_13030"
     CDS             <1..1076
                     /locus_tag="VKQ52_13030"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88168.1"
                     /translation="YKTYYQIAHDECQQIMARTDEHNLNPSFNTVFKGAIDAHTSDPA
                     GEIIFRVAMTGGAGSNDSKLGYYDGNKVNGLGNAAIGVLPTYFYLFDSVDTRRDVTCG
                     AYDVGKDFNILTPRTPTTLVEAKFRRDWMSNPSQLNSSAAQYFGLDWPLLRYSDVLLM
                     FAETDNELNNGPSPAAVTAFEQVRKRAYAGHTSAIGTTPTDYTGFFNAIVKERSLEFG
                     GEGIRKYDLIRWNLIAQRLADAKTNLTNMAAGKGGFTGSYNTNPLSFTTIPDSVFYDS
                     AKSVVSTGVLFKTSFYAKRTVSTDSGYKRILWASKAISGILSASGSSNGYAFYFTPNH
                     SELLPIPQQALDANYNLKQDYGY"
     gene            1066..>3248
                     /gene="pelA"
                     /locus_tag="VKQ52_13035"
     CDS             1066..>3248
                     /gene="pelA"
                     /locus_tag="VKQ52_13035"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HLZ88169.1"
                     /translation="MGIKRAAPVFVLSLLTAFTAPQSKTTIWLIGDSTIADKEIKAYP
                     ETGWGMPFEHFFDSTVMVDNRAKNGRSTKSFIAEGRWAAVMQTIKPGDYVLIQFGHND
                     EVPTKATYTPEDQFAGNLVRFIKESRDKRAVPILITPVARRKFDSAGNIQETHAAYAA
                     IVRRVAAEQQTPLIDLDRESQTLLQQFGPENSKWLFNYLLPGEHPNYPQGRQDDTHFS
                     EFGARKMAEIVLADIRNGNLDLADHIIRPPAPQARAAAPGIDPAPFADNAGHWYAIFD
                     KGNIINPLPGRPRHEPTDVKDIAENILLLQKTNGGWPKNYDPFAILTASQKDSLLDAK
                     NQTNTTFDNGSTYNQIMALATAWSALHDPRYSTAAIRGLNFILTAQYKNGGWPQYYPL
                     ESNYSRCITFNDGAYEGIMRLLKAIKDNQPQYDFVDDKLRQKLALAYEKGLDCILKTQ
                     ISDAGKPTAWCQQYDETSLKPAWARKFEPPSICNKESADLVLFLMSIDNPSPAIKAAI
                     GNAVAWFRASQILYTRVQTIPAERMVTPFRVSVSDRIVVTDSTAPPIWTRYYELKTHR
                     PIFCNRDSKIVYSLAEVARERRDGYGWYTYSPQRVLDEYPQWHNNQTTRHFTVPTDYP
                     TVQAAFDAAIPNTTILIKAGVYHEKLELTKDSITLIGETGAILTYDDHPGMVNPQGDS
                     INTRNSYSFRITGNDFTAENITFRNDAGFTAGQAVAVEARGDRAI"
BASE COUNT          785 a         1158 c          739 g          566 t
ORIGIN      
        1 actataaaac atactaccag atcgcccatg acgaatgcca gcagatcatg gcccgtaccg
       61 acgagcataa cctcaacccc agcttcaata cggtgttcaa aggcgctatc gatgcccata
      121 cctccgatcc cgccggcgag atcatcttcc gcgtagccat gaccggcggc gcaggctcca
      181 atgacagcaa actcggctat tacgacggta acaaggtcaa cgggctgggt aatgccgcca
      241 tcggcgtcct gcccacctac ttctacctct tcgactccgt cgacacccgc cgggatgtca
      301 cctgcggcgc ttatgacgta ggaaaagatt tcaacatcct gaccccgcgt acgcccacca
      361 cgctcgtgga agccaagttc agaagggact ggatgtccaa cccctcccag ttgaacagtt
      421 ctgcggccca atatttcggc ctcgactggc ccttgctccg ctactccgat gtgctgctca
      481 tgtttgccga aacagacaat gagctcaaca acggtccttc ccccgccgca gtcaccgcct
      541 tcgagcaggt gcgtaaacgc gcctatgccg gccatacctc cgctatcggg acgactccca
      601 ccgactacac cggcttcttc aacgccatcg taaaggaacg gtccctggaa ttcggcggcg
      661 aaggcatccg caaatacgat ctcatccgct ggaatctcat cgcccagcgc ctggcagacg
      721 caaagacaaa cctgaccaat atggcggcgg ggaaaggcgg ctttaccggc agttacaata
      781 ccaaccccct tagcttcact accatccccg actccgtgtt ctatgactcg gcaaaatccg
      841 tcgtctccac cggcgttttg ttcaagacct ccttttatgc caaaagaaca gtgtccaccg
      901 actcgggcta taagcggatc ctctgggcct cgaaagccat cagcggaata ctcagcgcat
      961 caggctcgtc caatggctat gccttctact ttaccccgaa ccatagcgaa ctgctgccca
     1021 tcccccagca ggccctggac gcgaactata acttaaagca ggactatggg tattaaacgc
     1081 gccgcaccgg tattcgtact ctccctgctg acggccttca cggcgccgca aagcaagacc
     1141 accatttggc tcatcggtga ctcgaccata gccgacaaag aaataaaggc ataccccgag
     1201 accggctggg gtatgccctt tgaacatttc ttcgactcta ccgtgatggt cgacaacagg
     1261 gcaaagaacg gccgcagcac aaagtccttc atcgcggaag gccgctgggc cgccgtcatg
     1321 caaaccataa aacccggcga ttatgtcctg atccagttcg gtcataacga cgaggtgccg
     1381 acaaaagcga cctatacccc cgaagaccag ttcgcgggca atctcgttcg cttcataaaa
     1441 gaatcccgtg acaagagagc cgtccccatc ctgatcaccc ccgtcgcccg ccggaaattc
     1501 gactctgccg gtaatatcca ggagacccat gccgcctacg ccgccatcgt ccgccgcgtg
     1561 gccgccgaac aacagacgcc gctgatcgat ctcgacaggg aaagccagac cctgctgcaa
     1621 caattcggac ccgaaaattc gaaatggctc tttaactacc tgcttcccgg cgagcatccc
     1681 aactacccgc agggaagaca ggacgacacc cacttcagcg agttcggcgc ccgtaagatg
     1741 gccgagatcg ttctcgccga catccgcaac ggtaacctcg acctcgccga tcatatcatt
     1801 cggccgcccg cgccgcaggc gcgggcggcc gccccaggca tcgaccctgc ccccttcgcc
     1861 gataacgccg gccactggta cgccatcttc gataaaggca atatcatcaa cccgctcccc
     1921 ggccggcccc gccacgaacc caccgatgta aaggacatcg ccgaaaatat cctcctcctc
     1981 cagaagacaa atggcggctg gccaaagaac tacgacccct tcgccatcct caccgcctcc
     2041 cagaaggata gcctgctcga cgcgaagaac cagacaaaca ccaccttcga caacggcagc
     2101 acctacaacc agatcatggc cctggccacc gcctggtcgg ctctccacga tccccgctac
     2161 agcacagccg ctatcagggg actgaatttc atcctcaccg cccaatacaa gaacggcggc
     2221 tggccacagt actatcccct cgaatccaac tacagccgct gcatcacctt caatgacggc
     2281 gcctatgagg ggatcatgcg cctgctcaaa gccatcaaag acaaccagcc tcaatacgat
     2341 ttcgtcgacg ataaactgcg ccaaaaactc gccctcgcct atgaaaaagg cctggactgc
     2401 atcctgaaga cccagatcag cgacgccggc aaacccaccg cctggtgcca gcaatacgat
     2461 gagaccagtc tcaaaccggc ctgggcgcgc aaattcgaac ccccatcgat ctgcaacaaa
     2521 gaaagcgccg atctcgtcct ttttctgatg agcatcgaca acccaagccc ggccataaag
     2581 gcggccatcg ggaacgccgt cgcctggttc cgcgcgtcgc agatcctgta cacccgcgtc
     2641 cagaccatcc ccgccgaacg gatggtcacc cccttccgcg tctctgtctc cgatcgcatc
     2701 gtggtcaccg actctacggc gccacccatc tggacccgct actacgaact aaaaacccat
     2761 cgccccatct tttgtaaccg cgacagcaag atcgtctatt cccttgccga ggtcgcccgg
     2821 gaaagaaggg atggctacgg ctggtatacc tattcccccc aacgcgtcct cgacgaatac
     2881 ccccaatggc ataacaacca gacaacccgt catttcaccg tccccacgga ctatcccacc
     2941 gtccaggccg ccttcgacgc cgcaataccc aatacgacga tccttatcaa agccggtgtc
     3001 taccacgaaa agctggaact cacaaaagac agcatcaccc tcatcggcga gaccggcgcc
     3061 atcctcacct acgacgatca tcccggcatg gtcaaccctc agggcgacag catcaacacc
     3121 cgcaactcct atagcttccg catcaccggc aacgacttca ccgcggagaa cattaccttt
     3181 cgcaatgacg ccggcttcac cgccgggcag gccgtagccg tggaggcccg cggcgatcgc
     3241 gccatctt
//
LOCUS       DATGFG010000698         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_49920, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000698 DATGFG010000000
VERSION     DATGFG010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49920"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VKQ52_13040"
     CDS             complement(<1..907)
                     /locus_tag="VKQ52_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLZ88170.1"
                     /translation="MLKVPHIKDSHSSDGVALPLDVLQARLRFLPGGLTTGEAELRLK
                     NYGLNEIAREKPLSPLRRLWNHLKNPLVILLSILGTVSWLTADISTTIVIFLMVVLGV
                     VLRYFQEMRADKAAEKLKAMVSTHATVVRDGKQSELPLRLLVPGDLVVLSAGDMVPAD
                     VRVLTAKDLFVNQAALTGEALPVEKSEMGPVVPGDNPLELTHTCFLGTNVVSGTATAM
                     VVNTGDHTYFGELACSISGEREPTSFDRGINRFTWLMIRFIFVMVPAVFLINGFSRHN
                     WLEAFLFAMAVAVGLTPEMLPMIVSV"
     gene            complement(918..1739)
                     /locus_tag="VKQ52_13045"
     CDS             complement(918..1739)
                     /locus_tag="VKQ52_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88171.1"
                     /translation="MKQVLFIGAGKEFPRGAFTFLRWMQDQEPLHVLGLFFCPMDYEA
                     TASACQLPVQGPYDRLVLAEQEAVQANKTLFARSCDQHYISYHLHENKGQWDKDLLIK
                     ESRFADLILLSGEGFYSDIRLEQPNFLLQEALQAAECPVMVIPEDYIQCDHLFFAYDA
                     SKESVFAIKQFSYLFPQLTDLPSEMVYVRDETSSTIPDLEHLRQYAKAHFNCIGFSKL
                     QFRAAHYFSTWISERKHVMLVTGSYGRNAFSYLAKRSFAEHVIHEHKLPVFIAHV"
     gene            complement(1753..2130)
                     /locus_tag="VKQ52_13050"
     CDS             complement(1753..2130)
                     /locus_tag="VKQ52_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88172.1"
                     /translation="MRANIMKSHVNRQFIREIERAFSAIYPFLKIEFPKEAPRWMTVE
                     EEGLDAAGWQRQAHDLLLNEIGLTDGMTVANLETRLLDLFAVPVVILRRSGNFWIETS
                     MTRQWSLKEQNDHGRELAGESSK"
     gene            complement(2194..>3070)
                     /locus_tag="VKQ52_13055"
     CDS             complement(2194..>3070)
                     /locus_tag="VKQ52_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HLZ88173.1"
                     /translation="DWPVKPKMPLIPGHEAIGYVAAVGQDVRNVKEGDIVGVPWLYSA
                     CGCCEYCITGWETLCEQQQNGGYSVDGGYAEYVIADARYVGKFPEGIDFVGMAPIICA
                     GVTVYKGLKETEARPGQWVAISGIGGLGHLAVQYAKAMGLQVAAIDIADEKLELAKKL
                     GASLVVNARKQDPGEYLKKEVGGMHGVLVTAVSPIAFKQGLSALRRKGTLSLNGLPPG
                     GFDLPIFETVLNRYTIRGSIVGTRQDLQEAIDFAVAGKVKATVHKARLEDINSILAEM
                     KKGEIEGRMVMEIGG"
BASE COUNT          684 a          979 c          750 g          657 t
ORIGIN      
        1 tgacggaaac gatcatgggc agcatctccg gggtgagccc gacagccacg gccatggcaa
       61 acaaaaaagc ctccagccag ttgtggcggg agaacccatt gataaggaaa acggcgggca
      121 ccatgacgaa aatgaaccgg atcatcagcc aggtgaaccg gttgatgcct ctgtcaaaac
      181 tcgtcggctc ccgctctccc gaaatactgc aggccagctc gccaaaatag gtatgatcac
      241 cggtattgac caccatggcg gtagcagtac cgctcactac attcgtgccc aaaaaacagg
      301 tgtgcgtcaa ctccagcgga ttgtcgccag gtacgaccgg gcccatttca ctcttttcca
      361 ccggaagcgc ttcccccgta agcgcagcct ggttcacaaa caggtctttc gcggtgagta
      421 cccgcacatc ggcagggacc atgtcgccgg cactcagcac cacgaggtca ccgggcacaa
      481 gaagtcgtag cggcagctcc gattgctttc catccctcac caccgtggca tgcgtgctca
      541 ccatggcctt cagcttttcc gccgccttgt ccgcccgcat ctcctggaaa taacgcagga
      601 cgacacctaa tacaaccatc aggaagatca cgatcgtagt actgatatcg gccgttagcc
      661 acgacacggt tcccaggatg gaaagcagga tgacaagcgg gtttttcagg tgattccaca
      721 aacgccggag gggagacaaa ggcttttccc tggcgatttc attaaggccg tagttcttca
      781 gccgcagttc cgcctccccg gtggtcagac cgcccggcaa aaagcgcagt ctcgcctgta
      841 gcacatccaa cggcagtgcg acgccgtcgg aggaatgact gtcttttatg tgcgggactt
      901 tcaacacgtt gcctgcatta gacatgagcg atgaagaccg gcaacttgtg ctcatggatc
      961 acatgctcgg caaaactccg tttcgcaagg taggagaacg cattccggcc atagctgccg
     1021 gtcaccagca tcacatgctt ccgctcgctg atccaggtgg agaaataatg cgctgcccgg
     1081 aattgcagct tcgagaagcc gatgcagttg aaatgcgcct tggcatattg ccgcagatgc
     1141 tcgaggtcgg gtatcgttga agaagtctca tcccgtacat agaccatctc ggaaggcagg
     1201 tccgttagct gcgggaacaa gtaactgaat tgtttgatcg caaaaacact ctccttactg
     1261 gcatcatagg caaagaacag gtggtcgcac tggatataat cctcgggaat gaccatcacc
     1321 gggcattccg ccgcctgcaa ggcctcctgc aacaggaaat tgggctgctc cagccggata
     1381 tcggaataga acccttcccc gcttagcagg atcaggtcgg caaaccggct ttccttgatg
     1441 agcagatcct tgtcccattg ccccttgttc tcatggaggt gataagagat atagtgctgg
     1501 tcgcaactcc gcgcgaacag ggttttattg gcctgcaccg cttcctgctc cgccagcacc
     1561 agccggtcgt aaggcccctg caccggcaac tggcaggccg aggccgtcgc ctcatagtcc
     1621 atcggacaga agaacaatcc gagcacatgc agcggctcct ggtcctgcat ccatcgcagg
     1681 aaagtaaatg ccccccgggg gaattccttg cccgcgccga taaataaaac ctgtttcata
     1741 gtagaagttt ttttatttac ttgattcacc ggccagttcc cgcccatggt cgttctgctc
     1801 cttcagggac cactgccggg tcatactggt ctcgatccag aaattcccgc tccgccggag
     1861 gatcaccacc ggcacggcaa acaggtccag cagcctggtt tcgagattgg ccacggtcat
     1921 cccgtcggtc aacccgatct cattcaataa aagatcatgc gcctgccgct gccagcccgc
     1981 cgcatcgagc ccttcttctt caaccgtcat ccacctcggc gcttccttgg ggaactcgat
     2041 cttcagaaag ggatagatgg ccgaaaaagc cctttctatt tccctgataa actgacggtt
     2101 gacatgcgat ttcataatat ttgctctcat accaaaagta tttcttgctg cacaatagaa
     2161 catgacgaac gacaggcgcc gatatgatcc ttatcatcct ccgatttcca tcaccatccg
     2221 cccttcgatc tcccccttct tcatctcggc taaaatggag ttgatgtcct ccagcctggc
     2281 cttgtggacc gtcgccttca ccttcccggc caccgcgaaa tcgatggctt cctgcaggtc
     2341 ctggcgcgtt cccacgatgc ttccccggat ggtatagcgg ttgagcacgg tttcgaatat
     2401 cggcaggtcg aacccccccg gcggcagccc gttcagggaa agagtaccct tccgccgcag
     2461 ggccgataac ccctgtttga acgcgatcgg cgaaacagcc gtcaccagta ccccatgcat
     2521 cccgccgact tccttcttga ggtattcgcc ggggtcctgc ttgcgggcgt tcaccaccag
     2581 gctggcgccc agtttctttg ccagttccag cttctcatcg gcaatatcga ttgccgcaac
     2641 ctgtagcccc atcgcttttg catactgcac tgcgaggtgg cctaaccccc ctattccgga
     2701 aatggcaacc cattgacccg gcctggcttc tgtctccttt aaccccttat agaccgtcac
     2761 cccggcacag atgatcggcg ccatccccac aaaatcgatc ccctcgggga atttgcccac
     2821 ataccgggca tcggcgatca catattccgc atacccgcca tccacactat acccgccgtt
     2881 ttgctgttgc tcgcaaagcg tctcccagcc cgtgatacaa tattcacaac aaccacaggc
     2941 gctgtacaac cagggtacgc cgacgatatc accctctttc acattgcgca catcctgccc
     3001 caccgccgcg acatacccga tcgcttcatg gcccgggatc aacggcatct tcggctttac
     3061 cggccagtcg
//
LOCUS       DATGFG010000699         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_4524, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000699 DATGFG010000000
VERSION     DATGFG010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4524"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VKQ52_13060"
     CDS             complement(<1..1576)
                     /locus_tag="VKQ52_13060"
                     /inference="COORDINATES: protein motif:HMM:NF016972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HLZ88174.1"
                     /translation="MRYQVLASDYDGTLADQGRVDPATEEKLRQLKATGRRLVLVTGR
                     EMKDLVLVFPEYRLFDYIVAENGGLIVDCATGEETPLGPPPDPKFIHRLRQSGVHPLS
                     IGKVIAATWVPYELAVIEAIKDTGSELQVIFNKGAVMILPSGVNKATGLQSLLRSLHL
                     SVHNAVAVGDAENDISLLQAAECAVAVNNALPSVKDMADWVTDERHGAGVAQLIDRLI
                     AGDLAGLDGKLTRHHLQLGETEKGPFTISPYEQGILISGVTGGGKSTFTTVIIEALVH
                     HGYQFCLIDPEGDYLQLPGVAIVGNETSVPTPEEIFQLLRDPDQSLVICTLSVPLADR
                     PAFFSRLLETMTRLRVEYGRPHWLILDEAHHLIPSASTMIADRMPIDFNNFIVISTSP
                     HALHPAVLEKIGTIITIGENPGYPFGQVGEILHLPTPEQIPTLIEGEICIWRLTTNQP
                     PFKARYPQPRQVMQRHKKKYATGDMGDNSFVFTGSEGRLHLVANNLMLFAHIAGGIDD
                     DTWSWHLHRHDYTNWFR"
     gene            complement(1579..2247)
                     /locus_tag="VKQ52_13065"
     CDS             complement(1579..2247)
                     /locus_tag="VKQ52_13065"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLZ88175.1"
                     /translation="MQLHTHPDTADGIGEGERVLSAIGGSLLVYYVTKKHKADTLLLL
                     GGAYLLYRAIAGHCPIRAAWKTNYRRAHPSNVNVRTQVIVNKPREEVYAFWRSLENWP
                     LFMRHLGNVDELDNTTSAWTMKMPGIGEVRWEARIVKEEKDTELSWTSVPGTPIDVTG
                     KINFSSTPGNATRIDVMLSYRAPAGIIGERISRLLTPSFQGKLEADIHNFKHYIEDLG
                     KTGD"
     gene            2467..>2686
                     /locus_tag="VKQ52_13070"
     CDS             2467..>2686
                     /locus_tag="VKQ52_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88176.1"
                     /translation="MYDLLQVGEEVAFPGLLDIKLIVEEVYENRGTVRCKYYDEHLKK
                     FIRLTLAADALVPSRKAPKQMKVSVSSKK"
BASE COUNT          503 a          646 c          890 g          647 t
ORIGIN      
        1 ccctgaacca attggtgtag tcgtggcggt gaaggtgcca ggaccaggtg tcgtcgtcga
       61 tgcccccggc gatgtgtgcg aagagcatca ggttgttggc tacgaggtgg aggcggcctt
      121 cgctgccggt gaagacaaaa ctgttatcgc ccatgtcgcc ggtggcatat ttctttttgt
      181 gtcgttgcat cacttgtcgt ggctgcgggt atcgggcttt gaagggtggc tggttggtgg
      241 tcaggcgcca tatgcagatc tcgccttcga tgagggtggg gatctgttcc ggcgtgggca
      301 ggtgaaggat ctcacccacc tgcccgaagg ggtagccggg gttctccccg atagtgatga
      361 tcgtaccgat cttttccagc acggcgggat gcagggcatg cggggaggtg ctgatgacga
      421 tgaagttatt gaagtcgatg ggcatgcgat cggcgatcat cgtggaggcg gagggtatga
      481 gatgatgtgc ttcgtcgagg atcagccagt gtgggcggcc atattccacg cgcagccggg
      541 tcatcgtttc cagcagccgg gaaaagaagg ccgggcgatc ggcgagagga acggaaaggg
      601 tgcagatgac gagggattga tcggggtccc gcagtaattg aaagatctct tcgggtgtgg
      661 ggacggaggt ttcattgccg acgatggcga caccgggcaa ctggagatag tcgccttcgg
      721 ggtcgatgag gcagaactgg tatccgtggt ggacgagggc ttcgatgatg accgtcgtaa
      781 aggtggattt gccgccgccg gtgacaccgg agataaggat gccctgttcg taggggctga
      841 tcgtgaacgg ccctttttcc gtttcgccca gttggaggtg gtggcgggtg agttttccgt
      901 cgaggccggc aaggtcgccg gcgatgagcc ggtcgatgag ttgggccacg cccgcgccat
      961 ggcgctcgtc cgtcacccag tcggccatgt ccttcaccga ggggagggcg ttgtttacgg
     1021 caacggcgca ttcggctgcc tgtaagaggg agatgtcgtt ctcggcgtca cccacggcca
     1081 cggcgttgtg taccgagagg tgtagcgatc gcagcagcga ctgcaatccc gttgccttgt
     1141 tcacgcccga gggcaggatc atgacggccc ctttgttgaa gatgacctgg agttcgctgc
     1201 cggtgtcctt tatcgcctcg atcactgcaa gttcgtaggg aacccaggtg gcggcgatga
     1261 cttttccgat cgagagcgga tggacaccgc tttgtcggag ccggtggata aattttggat
     1321 cgggtggcgg cccgagcgga gtctcttccc ccgtggcgca gtcgacgatg aggccgccgt
     1381 tctccgctac gatatagtca aagaggcgat actcggggaa gacgaggacg aggtctttca
     1441 tttcgcggcc tgtgacgagg accaatctcc ggcccgtggc cttgagctga cgcaattttt
     1501 cctccgtcgc ggggtcgacc cggccctggt cggcgagtgt gccgtcatag tccgatgcca
     1561 atacctggta gcgcatgatc aatcgccggt tttgccgagg tcttcgatgt agtgtttgaa
     1621 attatggata tccgcttcga gttttccctg gaaggagggg gtgagcaggc gggatattct
     1681 ttcgccgatg atgcctgccg gggcgcgata ggaaagcata acgtcgatgc gtgtggcgtt
     1741 gccgggtgtg gaggagaaat tgatcttgcc ggtgacatcg atgggggtgc cgggtacgga
     1801 tgtccaggac agttcggtgt ctttttcttc cttgacgatc cttgcttccc agcggacctc
     1861 gccgatgccg ggcattttca tcgtccatgc ggaggtggta ttgtccagtt cgtcgacgtt
     1921 cccgaggtgg cgcatgaaca gcggccagtt ctccaggctg cgccagaagg cgtatacttc
     1981 ttcgcgcggt ttattgacga tgacctgggt ccttacgttg acgttggagg gatgggcccg
     2041 ccggtaattt gttttccatg cggcgcgaat gggacagtga ccggcgatgg cgcgatacag
     2101 gaggtaggcg ccgcccagca gcaggagggt atcggccttg tgtttttttg tcacgtaata
     2161 aacgagcaat gagccgccta tggcggaaag gacgcgttcg ccttcgccga tgccgtccgc
     2221 ggtatccggg tgtgtgtgga gttgcataag acaattttta taacttttgc aaaatatttg
     2281 cctgatacga gtggggtatt actttcccac ctggcgccgt cggcgaatag ccctatgggg
     2341 ttttacttag gggggatttg gcaaggtcat acgtataatc acggataaaa agttgtctgt
     2401 tttaccacac tttcttacct tgttggacgt acgatctttg tcaaacctcc tttaaatttt
     2461 ctgcctatgt atgatctttt acaggtgggc gaagaggtag cctttcccgg cttgctggat
     2521 atcaagttga tcgtcgaaga ggtctatgaa aacaggggaa ccgtgcggtg taagtattat
     2581 gatgaacacc tcaagaagtt tatcaggctt acccttgccg cggatgctct tgttccttca
     2641 cgcaaggcgc ccaaacagat gaaggtctct gtcagtagca aaaaag
//
LOCUS       DATGFG010000700         3651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1485732, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000700 DATGFG010000000
VERSION     DATGFG010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485732"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VKQ52_13075"
     CDS             <1..915
                     /locus_tag="VKQ52_13075"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ88177.1"
                     /translation="QGASAPRMKKGALQRHLRRLNETEAPPSPAPRHRRFYLFAFTIA
                     ASLAAVFLFVYQWAPKKAVQPVAQNTVSTKPGSKSKIQLPDGSLVWLNADSRIIYNES
                     FRGAAREVQLCGEAYFDVVKDHNHPFIIHTHAIDVLVLGTTLNVRSYSNERNTEAVLI
                     HGSVQVTLRDNPAKKIILQPNDKLVVQNGRRLLLAPGGRAVQKEADTTQVMSLGKAHF
                     KAKDTVATEVLWVKNKLAFDEESLESVALKIERWFNVKVLITDPSLQHIEYSGVFEDE
                     TLPQVMEALRLTGNFHYTIHRREVTIRP"
     gene            1068..>3651
                     /locus_tag="VKQ52_13080"
     CDS             1068..>3651
                     /locus_tag="VKQ52_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ88178.1"
                     /translation="MKKTGSGPSPERVRLFIKYLLMTKLAILCVFALSIQSFARGYGQ
                     GNINLRLERTQLKKVFKAIEDQGFFRFVYKDEILPKDQRVSIVAREASVEEVLDKVLD
                     RTGLSYHRLTDNLIVIIRAAGTGEGAEKPLAAVKISGRVTNEKGEPLKDVSVVEKGTN
                     NGTLTKEDGSYSLDVTNPNGILLFSFVGYSPQEFPIKAKTAIDVRLLAGDNPLKDIIV
                     VGYASQKKVTVTGAVAMVKGSELEKTPTVNLSNALVGRLPGVYAVQSSGEPGYDGSTI
                     RIRGTNTLGNTSALVVVDGVPDIAGGLERLNPADIESMSVLKDASAAIYGARAANGVI
                     LVTTKHGKSGKPQLTYTFNHGWAQPDRIPKMASAPEYAAMNNETTVYDNVPYAEWTAA
                     WAAFQSTGTYTTQAGATVNAPFQPADIQKYKNGSDPWGHPNTDWFKTTLKTWSPQVQH
                     TLQINGGSENIRYLASLGYEDQDGYYKNSATGYKQYDMRLNLDAKVNKFINTGINLTA
                     REEYRFFPTQSAGSIFRMLMRGRPTDPEVWPNGLPGPDIENGQNPIVITTNQTGYDRD
                     KRDYFQVNGKVELTIPWVPGLKVTGTGTIDKENRIEKVWQTPWYLYFWDHATYEADGK
                     TPLLTKSMRSTFTTPQLSETNYNYLNILLSGFINYDKTFGDHTVNLMAAVTRETDNED
                     DFNAYRTNFISSAVDQLFAGGAPQQTVGGSAYERARLSYFGRAAYNYKEKYLAEFLWR
                     YDGSYLFPPAHRFGFFPGVLAGWRISEENFWKPLVHTVSYLKLRGSWGQMGNDQVYYN
                     GKLQEYQYLATYGFSNYVINNQAATTLYETVVPNPNFTWEVANNSDVGLEGQLLDGKF
                     NFELD"
BASE COUNT          855 a          990 c         1064 g          742 t
ORIGIN      
        1 cagggcgcct ccgcaccccg catgaaaaaa ggagctctcc agcgccatct ccggcggctg
       61 aacgaaaccg aagccccccc ttcccctgcg ccgcgacacc ggcgtttcta cctcttcgca
      121 tttactattg cggcttccct ggctgccgtg tttctttttg tctaccagtg ggcgccgaag
      181 aaagccgtcc agccggttgc ccagaatacg gtgagcacga agccgggttc gaagtccaag
      241 atccagttgc cggacgggtc gctggtatgg ttgaatgccg acagccggat catctataac
      301 gagagtttca ggggcgccgc gagggaggtg cagctttgcg gggaggcgta tttcgatgtg
      361 gtaaaagatc ataaccatcc ttttatcatc catacgcatg ccatcgatgt attggtcctt
      421 ggtacgacgc tgaatgtccg cagttattcc aacgagcgga atacggaggc cgtcctgatc
      481 catggttcgg ttcaggtaac gctgcgcgat aaccccgcca aaaagatcat cctgcagccc
      541 aacgacaagc tggttgtgca aaacggccgc agattgttgc tcgcccccgg cggcagggcc
      601 gtacaaaagg aggccgacac gacgcaggtg atgagtctgg gcaaggcgca tttcaaggcg
      661 aaggatacgg tggcgaccga ggtcctttgg gtaaagaata aattagcttt cgacgaagag
      721 agcctggaat ccgttgcttt gaaaatagaa cgctggttca acgtgaaagt tcttatcacg
      781 gacccctccc tgcagcatat cgagtacagc ggagtgttcg aggatgagac gctgccgcag
      841 gtgatggaag cgttgcggct gacggggaat ttccattata ccattcaccg gcgtgaggtc
      901 acgatacgac cttgattgat taaccaaaac gatacttact tatgaagaga gaaacctaac
      961 ccatcactga aataaaaaac ggagaatgat tgcagcatcc tccgctttgt aagacaatcc
     1021 ctgccgtttt accggacagg acattatcat tatctcaaat ctaagatatg aaaaaaaccg
     1081 gaagcggccc ttccccggaa agggttcgcc tattcatcaa atacctgctg atgacgaaac
     1141 ttgccattct atgcgtcttt gccttgtcta tccaatcctt tgccagaggg tatgggcagg
     1201 ggaatatcaa cctccggctc gagcggacgc agctgaagaa ggtgttcaaa gccattgagg
     1261 accaggggtt ctttcgtttt gtgtacaagg acgagatcct tcccaaggat cagcgggtca
     1321 gcatcgtggc cagggaggct tccgtcgagg aggtgctcga caaggtgctg gaccggacgg
     1381 ggctcagcta ccaccggctg acggataacc tgatcgttat catccgggcg gcgggcaccg
     1441 gcgaaggggc tgagaagcca cttgccgccg taaagatcag cggcagggtc accaatgaga
     1501 agggggagcc cttaaaagac gtcagtgtgg tggaaaaggg caccaataac ggcaccctga
     1561 caaaggagga tgggtcttat tccctggacg tgaccaaccc caacggcatc ctcctgttca
     1621 gttttgtagg ctattccccg caggaatttc ccataaaggc caagacggct atcgacgtgc
     1681 gattgctggc gggggacaat cccctgaagg atatcatcgt ggtcggttac gccagccaga
     1741 agaaggtgac ggtcacgggc gccgtcgcca tggtgaaggg gtccgagctg gagaagacgc
     1801 caacggtcaa cctctccaat gcccttgtcg gccggctgcc gggcgtctat gccgtccagt
     1861 cgagcgggga gcccggctat gatggttcga ccatccgtat ccgtgggacc aatacccttg
     1921 ggaacacctc ggcgctggtc gttgtcgacg gggtgccgga tatcgccggg ggcctcgagc
     1981 ggctcaaccc cgcggatatc gagagcatgt ccgtactgaa ggatgcctcg gctgccatct
     2041 atggggcccg ggccgccaac ggggtcatcc ttgtcacgac caagcatggc aagagcggca
     2101 agccgcaatt gacctataca ttcaaccatg gctgggcgca accggaccgt atcccgaaaa
     2161 tggcgagtgc gcccgaatat gcggcgatga acaatgagac gaccgtttat gataatgtgc
     2221 cgtatgccga gtggacggcg gcctgggcgg ccttccagtc gacgggcacc tatacgacgc
     2281 aggccggagc cacggtgaac gcacccttcc agccggccga tatccagaaa tacaagaacg
     2341 ggtcggatcc ctggggccat cccaacacgg actggttcaa gacgacgctg aagacctggt
     2401 cgccccaggt gcagcatacg ttgcagatca acggcggctc ggaaaatatc cggtacctgg
     2461 cctccctcgg atatgaggac caggatgggt attataagaa ttcggccacg ggttacaagc
     2521 aatatgatat gcggttgaac ctggacgcca aggtgaacaa gttcatcaac accggtatca
     2581 atctcaccgc ccgggaggaa tatcgcttct ttccgacgca gtcggcgggt tccatcttcc
     2641 ggatgctgat gcggggaagg cccaccgatc cggaggtttg gccgaacggc ctgccggggc
     2701 cggatatcga gaacgggcag aaccccatcg tgatcacgac gaaccagacg ggatacgatc
     2761 gcgataagcg cgactatttc caggtgaacg gcaaggtgga attgacgatc ccctgggtgc
     2821 cggggttaaa ggtgacggga accgggacca tcgacaagga gaaccgcatc gagaaagtat
     2881 ggcagacgcc ctggtatctt tatttctggg atcatgccac ctacgaggcg gatggcaaga
     2941 cgccgctgct gaccaagagc atgcgttcca cttttacgac accacagttg tcggagacca
     3001 attacaatta cctgaacatc ctgctttcgg gctttatcaa ttatgacaag acctttggtg
     3061 accataccgt caacctgatg gcggccgtca cacgggagac cgacaacgag gatgatttca
     3121 acgcctatcg caccaatttc atttcttccg ccgtggacca gttgtttgcc ggtggtgcgc
     3181 cccaacagac ggtgggcgga tcggcctatg aacgggcccg gctcagctat ttcggccgtg
     3241 ctgcctataa ctataaggaa aaataccttg cagaattcct atggcgttat gatggttcct
     3301 atctcttccc cccggcgcac cggttcgggt tcttcccggg tgtgctggcg gggtggagga
     3361 tctccgagga gaatttctgg aaaccccttg tccatacggt cagctacctg aagttacgtg
     3421 ggtcctgggg acagatgggg aatgaccagg tgtactacaa cggtaagctg caggagtacc
     3481 agtatcttgc cacctatggt ttcagcaact atgtgatcaa caaccaggcg gctacgaccc
     3541 tgtacgagac cgttgtaccg aaccccaatt tcacctggga ggtcgccaac aattcggatg
     3601 tggggctgga agggcagttg ctggacggga agttcaattt cgagctggat t
//
LOCUS       DATGFG010000701         4682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_464104, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000701 DATGFG010000000
VERSION     DATGFG010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464104"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VKQ52_13085"
     CDS             complement(<1..1160)
                     /locus_tag="VKQ52_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88179.1"
                     /translation="MSVNAISAKIAWRNILRQRWYSLIHVLGLAIGMCVCIVIYLIGR
                     YELSFDSFHPDANRIYRIVGDVQDKDGNVIFLNSPFREVAGIEHLIAGFEARTAFHVF
                     GSTISVPSINSQVTKVYDNRQEGSYALSTILTAPDFFRLFPHQWLAGTPSVLDKPGRL
                     VLAESVARKYFGTDKIDQVLGRKVIYDDSLPLTVAGIVRDWDRPSDLNYTSFISISTA
                     PETWLRERIPTADWSSLQPRRSQAFVLLEKGIDPRRVNAQLAEYLHRLTPAPVLFSGA
                     NHLRLYLQSLRDMHYTPNFHPADTGDDFPKAYLPLLYALMWVALFVLGLAIINFINLS
                     SAQSFQRMKEVGIRKVMGSSRKALAGQFLVETLLLTVCALVLSVLLVLPVLWL"
     gene            complement(1168..3579)
                     /locus_tag="VKQ52_13090"
     CDS             complement(1168..3579)
                     /locus_tag="VKQ52_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ88180.1"
                     /translation="MIVHFFKTAFRSLRANKVYSILTVAGLGVGIAVFLVIFLFIRFQ
                     ESYDRFHAKKANIYRILTTGDEPGDHPGSAAPHPMPAALKHDFPDWKVTGIFALDGLQ
                     VQTLDETGKVENAFKEDGGAFFVDSSFFSIFDFPWLAGAPDKAMADGESVILTKSTAD
                     RYFGDWHKAMGRVLNFRGTPGADKPRRPARVTGILADPPANTDFQLKVVFPYPMLHFD
                     KHWWWTLDDANQCYVLLPAGADTATLNRQLAALSKKYRDPKDKHGQIVEPLADVHYNN
                     EAATFSGAITHTRIRSLWLIAGFVLLIACVNFVNISTAQAVNRAREVGVRKVLGGSRW
                     QLRKQFLLEAGMLVAGGVLLAVFLISLLLVPISRVLEIPLSLHLFQEPKVLLFLAVTA
                     AVVTLLAGSYPAMVISAFKPVTALKAKLVARSNQGLNLRRGLVIVQFLIAQALIMGTL
                     LVVQQLSFFLNAPMGFDKSAVISVSIPVDSLSRTKLDYLRNQLLAIKDVRAVSYNSSA
                     PASDNIWGGPFKFDHAAEDAPFPTIRASIDANYFSTYSMPLVAGRNITRTDSIEEFVV
                     NLAMVEKLGFARPEDVLNKQMRLGSEVGPIVGVVKNFHIASLKSGSVLSPVVMRKNPD
                     DWRSAGIRLDGKNMTATIGYIRRLWRQVYPEYAFDYQFLDDRVAGFYADEIKLSGFYK
                     IFAAIAIFLSCLGLYGLVSFMAAQRRKEVGIRKVLGATVGQIVSLFTREFIWLVGIAF
                     ILATPLAWYFVHKWVEDYASRAPIGIWLFVLSGVGALAIALATVGYQALRAARMNPVE
                     NLRTE"
     gene            3794..>4682
                     /locus_tag="VKQ52_13095"
     CDS             3794..>4682
                     /locus_tag="VKQ52_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88181.1"
                     /translation="MPHTKPLSVLLILALFVLSCKKDPVVSGNNPTQPHDTTVAPVPT
                     PKLHMSLSYLIRKHTIDSTWRTNYELYISEAGGKLLLDTMMDYNRTITADLSTGAKFL
                     DVTVIYSSYPMGATPQFFVHTYKTVDLAQWKNLPLSDSVPGQVYPFVTGTATMQLENV
                     AAPTPFLWQFMCNDDPGPTFLPSSGPYFDPQGISYSSNLTVSYPWEQDDYAYIAFPYN
                     GLYKLHKIGGQNDTADLSQLDTAKTVSFNWPAQYDIMPVLFGYMDTTNRNKSLLLAPG
                     HGQYTHNYTYETMYPADKKV"
BASE COUNT         1148 a         1397 c         1130 g         1007 t
ORIGIN      
        1 agccacagca ccggcaatac cagcaacacg gaaagtacca gggcgcatac cgtcagtagc
       61 aatgtctcga ccaggaactg gccggcaagc gctttcctgc tgctgcccat cacttttcgg
      121 atgcccacct ccttcattcg ctggaagctt tgggcactcg agagattgat gaaattgatg
      181 atggccaatc caagaacgaa tagcgccacc cacatcaacg catacaggag cggcaggtag
      241 gcctttggga aatcgtcacc cgtgtccgct gggtgaaaat tcggcgtata atgcatatcg
      301 cgcagcgatt gcaggtacag ccgcagatga ttggcgccgc taaaaagcac cggcgcgggc
      361 gtcagtcggt ggaggtattc ggccaattgc gcatttacac gccgggggtc gatccctttt
      421 tccaacagca caaacgcctg cgagcgccgt ggttgcagac tgctccagtc cgcagtggga
      481 atacgttcgc gcagccaggt ttccggcgcg gtacttatcg agataaaaga agtgtagttg
      541 aggtcagaag gccgatccca atcccgtacg atacccgcca ccgttagcgg cagactgtcg
      601 tcatagatca ccttccggcc cagtacctgg tcaattttat ccgtcccgaa atatttccgc
      661 gccacacttt cagccaacac caaccgtccc ggcttgtcca gcaccgaagg ggtgcccgcc
      721 agccactgat gtggaaagag gcgaaagaaa tccggtgccg tcaaaatggt ggatagtgca
      781 taactaccct cctgtctatt atcgtagacc ttcgtgacct ggctatttat cgagggtacc
      841 gagatggtcg agccaaagac atggaaggcc gtcctggctt caaatcccgc gatcaggtgt
      901 tcgatccctg ccacctcccg gaacggactg ttgagaaaga tcacattgcc atccttatcc
      961 tggacatcac ctacaatgcg atagatcctg tttgcatcag gatgaaaact atcgaagctc
     1021 agctcatagc ggccgataag atagatcacg atacaaacgc acattccgat ggccagccca
     1081 aggacatgga taagcgaata ccaccgttgc cgaaggatgt ttcgccaggc tattttcgct
     1141 gagatggcat tgacagacat acgattttta ttccgtcctc aaattctcca cgggattcat
     1201 cctcgccgcg cggagtgcct ggtaacccac tgtcgccagc gcgatcgcca gggcccccac
     1261 cccactcagg acaaacagcc atattccaat cggcgcccgg gaagcataat cctcgaccca
     1321 tttatgaacg aaataccagg cgagcggcgt cgcgagtatg aacgcaatcc cgaccaacca
     1381 tataaattcc cgcgtgaaca gagatacgat ctgccccacg gtagccccta aaaccttgcg
     1441 tatccctacc tctttccgcc gctgcgccgc cataaagctc accaacccat ataatccaag
     1501 gcagctcaga aagatggcga tcgccgcgaa tattttatag aaacccgaaa gctttatctc
     1561 gtccgcatag aaaccggcaa ccctgtcatc caggaattgg tagtcgaagg catattccgg
     1621 atacacctgt ctccagagtc gccggatata cccgatggtg gccgtcatat ttttcccatc
     1681 cagccggatg ccggcagaac gccagtcgtc cgggttcttc ctcatcacta ccggggacag
     1741 caccgatccc gacttgagcg aagcaatatg gaaattcttc accacaccca cgatcggtcc
     1801 gacttcactg ccaaggcgca tttgcttgtt caacacatcc tccggccgcg caaaccccag
     1861 tttctctacc attgccaggt tcacgacaaa ctcttcaata gaatcggtgc gtgtgatatt
     1921 ccggccggcc accagcggca tggaataggt ggagaaataa tttgcatcga tggatgcacg
     1981 gatggtgggg aacggcgcgt cctccgcagc atggtcgaat ttgaagggtc cgccccagat
     2041 attatcgctg gcgggcgcac tgctgttgta gctcaccgcc cggacatctt tgatcgccag
     2101 caactggttg cgcagatagt cgagcttcgt tcggctgaga ctgtctacgg ggatcgatac
     2161 acttatcacc gcgcttttgt cgaaacccat gggagcattc aaaaagaaac tcagctgctg
     2221 caccaccagc aaggttccca tgatcagcgc ctgcgcgatc aaaaactgga cgatgaccag
     2281 tccgcgtcgc agattcaagc cctggttgct tcgcgccacc aatttggcct tcaacgcggt
     2341 gaccggctta aatgccgaga tgaccatcgc cggataggac ccggccagca gcgtaaccac
     2401 cgccgctgtg accgccagga acaataatac cttcggttcc tggaaaagat gcaacgacag
     2461 ggggatctcg agcaccctgc tgatcggaac gagcaaaaga ctgataagga acaccgcgag
     2521 aagaaccccg ccggcaacca acattccggc ctccagcagg aattgctttc ttagctgcca
     2581 ccggcttccc cccaatacct tccttacgcc aacctctctg gcccggttca ctgcttgcgc
     2641 cgtgctgata ttcacgaaat tcacgcaggc gatgaggagg acaaagcctg cgatcagcca
     2701 aaggcttcgg atgcgcgtat gggtaatagc cccgctgaaa gtagccgcct cgttgttata
     2761 gtgaacatcg gcaaggggct ccacgatctg gccatgcttg tccttcggat cgcggtattt
     2821 cttcgataga gcggctaatt gccggttgag ggtggcggta tccgcgcccg ccggcagcaa
     2881 tacataacat tgattggcat catcgagcgt ccaccaccaa tgtttgtcaa aatgcagcat
     2941 agggtatgga aacaccacct ttagttggaa atcggtattc gcgggcggat cagccaaaat
     3001 gcccgttacc ctggcaggcc gtcgtggttt gtcggccccg ggagttcccc gaaaattgag
     3061 cacacgcccc attgccttgt gccaatctcc gaaataccgg tctgccgtgc ttttggtcaa
     3121 tattaccgat tccccgtccg ccatcgcctt atccggcgct cctgccagcc agggaaaatc
     3181 gaaaatggag aagaacgaac tgtccacgaa aaaagctccc ccatcttctt tgaatgcatt
     3241 ctccaccttt cccgtttcgt ccaatgtctg gacctgtaaa ccatccaatg caaaaatgcc
     3301 cgtcaccttc cagtccggaa aatcatgctt caatgccgcc ggcatcggat gaggtgctgc
     3361 tgagccggga tggtcgccag gttcatcccc cgtcgtcaat atccggtaga tgttcgcttt
     3421 ctttgcatgg aacctgtcat aactctcctg gaaccggatg aacaggaaga tcaccaggaa
     3481 aaccgcaatg cccacaccga ggcctgccac cgtcaaaatg gaatagacct tgtttgccct
     3541 caaactcctg aaagcggtct tgaagaaatg cacgatcatg gagaagtgat ttcctactaa
     3601 ccagcagcaa cgatgccggt ttgccatgta attgatcatc aacctattcg tcccgtgttc
     3661 gccacccatc ctgtccgctt tcgttacatc gcctgtccgc aaaccgaagt gacggtgact
     3721 gctgatcgcg gactccgggt gattggaaaa tctgggaatt atttgtagat tagcctccag
     3781 ctaaaccttc aagatgccac ataccaagcc cttatccgta ttactgatcc ttgccctatt
     3841 tgtactttcc tgcaaaaaag acccggtggt ctccggcaac aatcccacac aaccgcacga
     3901 caccacggtc gctcccgttc caaccccgaa gttgcacatg agtctttcct acctgatcag
     3961 gaaacacacg atcgacagta cgtggagaac gaactacgag ctgtatatca gcgaggccgg
     4021 cggcaaactc ctgctggaca cgatgatgga ttataacaga acgatcactg ccgacctgtc
     4081 gacaggcgcg aaattcctgg atgtcaccgt catctactcc tcctacccaa tgggagcaac
     4141 accgcaattt tttgtccaca cctataaaac ggttgacctc gcgcagtgga aaaatctgcc
     4201 cttatccgat tccgttccgg gccaggtgta ccctttcgtc acaggcaccg cgacaatgca
     4261 gttggaaaat gtcgccgctc caacgccgtt tttatggcag ttcatgtgca atgacgaccc
     4321 cggaccgaca ttcctgccca gcagcggccc ctatttcgac ccgcagggca tctcctactc
     4381 cagcaatctt accgtcagtt atccctggga gcaagatgac tatgcctata tcgcgtttcc
     4441 ctataatggt ctgtacaaat tacataagat aggcggccaa aatgatacgg ccgatctttc
     4501 gcagttggat accgcgaaga ccgtcagctt caactggcct gcgcaatacg atataatgcc
     4561 ggtactgttt ggctacatgg atacgaccaa tcgtaacaag tcgctgctcc tggcgccggg
     4621 tcatggacag tatacgcaca attataccta cgagacgatg tatccggccg acaagaaggt
     4681 ct
//
LOCUS       DATGFG010000702         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_124793, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000702 DATGFG010000000
VERSION     DATGFG010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124793"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /gene="glgP"
                     /locus_tag="VKQ52_13100"
     CDS             <1..1229
                     /gene="glgP"
                     /locus_tag="VKQ52_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HLZ88182.1"
                     /translation="CHRLYDSNPETQLAAAILLGQGGARLLEEIGHTPDIYHLNESHG
                     LPLAFYLYDKYRNMEEVRRRMVFTNHTPEPSGNQQSEINQLARMGYFGNLTLEEVRQV
                     SGAGNDLLDHTLTALRSAGRANGVSQMHLQTLNRIWAGQSDICPLTAVTNAQSADFWQ
                     DREMYAALMTSDDAALRVRKKACKEKLFEEVADQCGTLFSSDILTIVFAKRFTGYKRP
                     ELLLHDLDFLQQLLSDPQRPVQLIWAGKPYPMDYGSIGSFDRLVNLCKTYPNVAILTG
                     YELRLSRLLKQGADIWLNTPRMTHEACGTSGMSAAMNGAINVSIPDGWFPEFARDGIN
                     SFVIPDTEVTEHKFQQDETDAHNLYALLKNTVIPLYYDHPDQWLTIVKNSMRDILPQF
                     DSRRLAAEYYEKLYWS"
     gene            complement(1226..1696)
                     /locus_tag="VKQ52_13105"
     CDS             complement(1226..1696)
                     /locus_tag="VKQ52_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88183.1"
                     /translation="MPVILSVAGFGLLLFALLRKQPLLVSTAYWVLLIAAVAALPVYL
                     TGEGTAERLEKLPGITENSIESHEEVAESAFIAIELVGVLSILGLIFQKRIKLGKALR
                     LLTLLFAFVAAAVLAEAAHLGGQIRHSEIRKDAQPQSKGANSPGEANAPRKGDD"
     gene            1847..2233
                     /locus_tag="VKQ52_13110"
     CDS             1847..2233
                     /locus_tag="VKQ52_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88184.1"
                     /translation="MPVKKAIAILLMSVYLFGTTEAYQALKLPLLVRHYIKHKHENPN
                     LTLLGFLKLHYSDKTVFDADYQQDMKLPFKTQENTSLFSAVNDIPRPLIITVESPLSA
                     SADYILLDARVDISAFPHSVFQPPRA"
     gene            2427..>3011
                     /locus_tag="VKQ52_13115"
     CDS             2427..>3011
                     /locus_tag="VKQ52_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ88185.1"
                     /translation="MLNAIISFSVRNKLIIGIFVLSLIGWGAYSVTRLPIDAVPDITN
                     NQVLVITSSPSLGAPDVERFITVPVEQATRNVPGIVEQRSFSRFGLSLVTIVFNDATD
                     VYWARQQVSERLAQARQQIPASMGDPQLGPVTTGLGEIFQYAVRPKPGYEDKYDLNAL
                     RDIQDWIIRRQLLGTEGVADVSSFGGKVRQYEVTV"
BASE COUNT          723 a          854 c          728 g          706 t
ORIGIN      
        1 tctgtcatag gctatacgac tcgaatccgg aaacccaatt ggccgcggcc atattgctcg
       61 gccagggtgg cgcccgcctg cttgaagaaa tcggccatac gccagacatt taccacttga
      121 atgaatccca cggccttccg ctggcgtttt atttgtacga taaatatagg aatatggaag
      181 aggtgaggag gcgaatggta tttaccaacc atactcccga accatcggga aatcagcaat
      241 cggagattaa ccagcttgcc cgaatgggct atttcggtaa tctcaccctc gaggaagtca
      301 ggcaggtctc gggcgccggg aacgatctcc tcgatcatac cttgacagcc ttacgatcgg
      361 ctgggcgggc gaacggcgta tcccaaatgc acctgcaaac attaaataga atatgggccg
      421 gccaaagcga tatttgcccg ctgacggctg tcactaatgc gcaatccgcc gacttctggc
      481 aagaccggga aatgtatgcc gccttaatga catcggatga tgccgctctc cgtgttagaa
      541 aaaaagcctg taaggagaag ctgtttgaag aagtagccga ccaatgcggc acactcttca
      601 gttccgatat tctcactatt gtctttgcca aacgcttcac cggctacaag cgcccggaac
      661 tcctcttgca tgacctggat tttctccagc aactgctatc cgacccgcaa cgacccgttc
      721 aactcatttg ggccggtaag ccttatccta tggattatgg ctccatcggc agtttcgaca
      781 ggttggtgaa cttatgcaag acctatccca atgtcgctat tctcacaggc tatgagctcc
      841 gcttatcaag gctgctaaag caaggcgccg atatatggct caatacaccc cggatgacgc
      901 acgaggcgtg cggtaccagc ggaatgtccg cagcaatgaa cggagcaata aatgtatcca
      961 taccagacgg ctggttccct gaatttgcca gggatggcat taattctttc gtaatccccg
     1021 acaccgaggt gacagagcac aaatttcagc aggacgaaac ggatgcacac aatctctatg
     1081 cgttgttaaa aaatacggtt atccccctgt attacgatca tccggatcaa tggctgacta
     1141 tagtaaaaaa tagtatgcgg gatattctgc cacagtttga tagtcggcgg ttggcggccg
     1201 aatactacga aaaattatat tggtcttagt cgtctccctt gcgtggtgca tttgcctccc
     1261 cggggctatt cgcccctttg ctctgcggct gggcatcttt cctgatctcg ctatgccgga
     1321 tttggccacc caggtgagcc gcttcggcca agacggccgc cgctacgaag gcaaacaaca
     1381 atgtcagcaa tcgcagcgcc tttcccagct tgatacgctt ctgaaagatc agccctagaa
     1441 tgctgagtac acctaccagc tcgatagcaa taaacgcaga ctcggctacc tcctcgtgcg
     1501 attcaatgct attctcggta atgccgggta gtttttccag cctttccgcg gttccctccc
     1561 cggtgaggta aacgggtaag gctgccacgg cagcgatcag caacacccaa taagcggtcg
     1621 aaaccagcag gggctgcttg cgaagaagcg caaatagcaa caagccgaag ccggctacag
     1681 acaatatcac tggcacatgc gtcagagcga gatgtaattg aacgggattc atacaattta
     1741 tttgtataaa ggttgcaaca ttctggcagg atcggcttga tctccatcat atatcccaat
     1801 gatcttatac gccaatgctc gccactcaaa aaattatcct acctttgtgc ccgtgaaaaa
     1861 ggcgatcgcc atactattga tgtctgtcta cctcttcggt acgaccgagg cctatcaggc
     1921 gctcaaattg cctttgctgg tccggcacta tatcaaacac aaacacgaga acccgaacct
     1981 gaccttgctg ggcttcctta agcttcatta ttccgataaa acggtttttg atgccgacta
     2041 ccagcaggat atgaagctgc ctttcaagac gcaggagaat accagccttt tctccgcagt
     2101 aaacgatatc ccgcgccctt tgatcatcac tgtcgaaagt ccgctgtcgg cctcggcaga
     2161 ttatatcctg ctggacgccc gtgtagacat ttctgctttt cctcactccg tctttcagcc
     2221 tccccgggcc tgaattcatt ccctcctttt tattaacggc cattatcgcg gtcagggcag
     2281 cgatgcgtta actcgctatt acgcacctgc cggcagacgt attgcgcaga acaggcgccg
     2341 ctgacgcggc gcagcgccct tacctgccca ggctttgcca gtgaacccgt tggccgattt
     2401 gatcacgctc atagtactgt atacgcatgt taaacgctat catcagcttt tccgtgagga
     2461 ataagctcat catcggcata ttcgtccttt cactcatcgg ctggggagca tattcagtga
     2521 cccggttgcc catcgatgcg gttcctgata ttacgaacaa ccaggtgctg gtcatcactt
     2581 cctcacccag cctcggcgcg ccggacgtcg aacgctttat taccgttccg gtagaacagg
     2641 ccacccgcaa tgttcccggt atcgttgaac agcgaagctt ttcccgcttc ggccttagcc
     2701 tggtcaccat tgtatttaac gacgccaccg atgtttactg ggcccgccag caggtcagtg
     2761 aacgactggc ccaggcgcgg caacagatac cggccagcat gggcgatcct caactgggac
     2821 cggtgaccac cggcctgggc gagatctttc aatacgccgt ccgacccaaa ccgggttacg
     2881 aagacaaata tgatctgaac gccctgcggg acatccagga ttggatcatt cgccggcagc
     2941 tgcttggcac cgaaggagtc gccgatgtca gcagtttcgg cggaaaggtc cgtcaatatg
     3001 aagtgacggt c
//
LOCUS       DATGFG010000703         5332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1008265, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000703 DATGFG010000000
VERSION     DATGFG010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008265"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VKQ52_13120"
     CDS             <1..295
                     /locus_tag="VKQ52_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88186.1"
                     /translation="DADIGDECIVGALSLVKTGEKVPPRTVVAGNPARIIRPVSEEML
                     RWKTEGTAIYQALPGEMQGGWKVCEPLSPEEVPAGESKTEEPGREYKPWKGTE"
     gene            312..1325
                     /gene="obgE"
                     /locus_tag="VKQ52_13125"
     CDS             312..1325
                     /gene="obgE"
                     /locus_tag="VKQ52_13125"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HLZ88187.1"
                     /translation="MEKSNFVDQIRIFCRTGHGGAGSKHFMRTKFNAMAGPDGGDGGR
                     GGHIILRGSKDQWTLLHLRYYKNVLAEDGEAGSGNNCTGRYGKDIFIDVPLGTVARDE
                     ETGKVEAEILEDGQEIVWMKGGRGGLGNASFATPTNQAPEHAQPGLPGVEGWKVLELK
                     VLADVGLVGFPNAGKSTLLSVITAAKPKIADYAFTTLTPQLGMVEYRDGRSFCMADLP
                     GIIEGAAEGKGLGHRFLRHIERNPVLLFLIPADSPNHSEEFRILVHELEKYNPELLHK
                     RFIIAISKSDMLDDELKEAITRELPPDIPHLFISSMINQGILELKDLLWKVLNEDVTN
                     SNK"
     gene            1590..2003
                     /locus_tag="VKQ52_13130"
     CDS             1590..2003
                     /locus_tag="VKQ52_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88188.1"
                     /translation="MKSLFIMRFGKLLLGASFLLILATAHGQSLVSSSGSGDSAVTVR
                     YLGVQDDLLVFNVAYSNPDGSRFQLTVKDQDGAQLYQSIFNDKAFFKQFRLPKSDKDR
                     MVFIFHDYKDADIVKAFDVNVNSRFVREVAVRKVD"
     gene            2048..2437
                     /locus_tag="VKQ52_13135"
     CDS             2048..2437
                     /locus_tag="VKQ52_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88189.1"
                     /translation="MKSIVLSLLFAGVSLVSLANGHDPIGDKAASTAEVKYITAGNGA
                     FFFNVTYNNATGGRFSVVVLDEYGNQLYQNVYTDKKFDKKFMLADPDNFKKLTFVIRN
                     YSDNTVQRFEVNSDDRMVEDVEVKEVQ"
     gene            complement(2500..3012)
                     /locus_tag="VKQ52_13140"
     CDS             complement(2500..3012)
                     /locus_tag="VKQ52_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88190.1"
                     /translation="MSKLKIVLSALIVIGSLSSLFSVVGCKKTTTNVVKDSVYYSPWL
                     TISMKATDAGDTAYYQDITAKTVTAAVIRSGAVLSYLGSPASGDTTVAPASDYGLYQL
                     LNVGNIEIESFGYLNDFSTSNSSLLYRYVVIPGNVLASTSLKNFSQQQLSKMTFTDIQ
                     KAASTPVQGR"
     gene            complement(3134..3757)
                     /locus_tag="VKQ52_13145"
     CDS             complement(3134..3757)
                     /locus_tag="VKQ52_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88191.1"
                     /translation="MEYLLHLSKDKKLKKLIGDHEPVRIKKQKDIHFYLIASIMSQQL
                     STKVANTIRQRFLALYGDRVPTPEEVLATPFETLRSIGLSNAKARYVQNVAQFAMEMG
                     MDHKKLGRMDNEEVIAWLTQIKGVGRWTAEMLLMFSLGREDVFAIDDWGVQNAMIHLY
                     NLDRSDKKAFREDLLRISAKWSPYRTYACMHLWRWKDNRPAIAAKKK"
     gene            complement(3779..5290)
                     /gene="cysS"
                     /locus_tag="VKQ52_13150"
     CDS             complement(3779..5290)
                     /gene="cysS"
                     /locus_tag="VKQ52_13150"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HLZ88192.1"
                     /translation="MSQLKVHNSYTRQKEVFTPITPGHVGMYVCGPTVSGESHLGHAR
                     PTITFDFIYRYLLHLGYKVRYVRNITDAGHFEEEGREAEDKIAKKAVLEKLEPMELVQ
                     KYTNLFHWAMRQFNNLNPSIEPTATGHIVEQISMIKTIIREGYAYEINGSVYFDVKKY
                     AAHYPYGKLSGRVLDDLLETTRDLDGQDEKHNRQDFALWKAAPPEHIMRWQSPWGEGF
                     PGWHIECSAMSSKYLGAQFDIHGGGMDLQFPHHESEIAQSTICNKEIPARYWLHNNMI
                     TLNGKKMGKSYGNQIRLTEMFTGTHPLLQQAYTPMTIRFFILQTHYRSTLDFSNEALQ
                     AAEKGLKRLWEAYDLLKKLAADDAGGNGSPATAADPSIDDNVRKLLHELDEFMNDDFN
                     TAKVLANLFELVPIVNSLKGGQIRRDALSPATLQLMKRYFTDYLENILGLKGESLGDD
                     QKLSGVMELLIDIRRDARSRKDYSTSDKIRNQLLQLGIVLKDEKDGGVSYTFA"
BASE COUNT         1139 a         1288 c         1564 g         1341 t
ORIGIN      
        1 cgatgccgac atcggtgatg agtgtatcgt gggggcgttg agtcttgtaa agacggggga
       61 gaaggtgccg ccacggacgg tggtggcggg gaatccggcc aggatcatcc ggcctgtgag
      121 cgaggagatg ttgcgctgga agacggaggg gacggcgatc taccaggcgt tgccggggga
      181 gatgcaggga ggatggaagg tttgtgagcc attgtccccg gaggaggttc cggcgggtga
      241 gagtaagacg gaagagcccg ggcgcgagta taagccgtgg aaaggaacgg agtaagtatc
      301 tttgcagccg aatggagaaa tcaaattttg tcgaccagat caggatcttt tgcagaaccg
      361 ggcacggagg cgccggcagc aagcatttta tgcgaacgaa attcaatgcc atggcggggc
      421 cggatggtgg ggatggcggg cgtggcgggc atatcatcct tcggggaagc aaggatcagt
      481 ggaccctgct gcacctgcga tattataaga atgtgctggc ggaggatggc gaggccggga
      541 gcggcaataa ctgtacgggg cgctatggca aggatatttt tatcgatgtg ccgctaggca
      601 cggtggcgcg ggatgaggaa accgggaagg tggaggcgga gatcctcgag gacggccagg
      661 agatcgtctg gatgaagggc ggtcgcggcg gtcttgggaa tgccagtttc gccacgccga
      721 caaaccaggc gccggagcat gcgcagccgg gcctgccggg tgtggagggt tggaaagtat
      781 tggaattgaa ggttttggcc gatgtgggtc ttgtcggttt tcccaatgcc ggtaagtcta
      841 ccctgctgtc ggtgatcacg gctgccaagc caaagatcgc cgactatgcc tttacgacgt
      901 tgacgccgca gctggggatg gtggagtacc gggatggccg gtcgttctgt atggccgatc
      961 tgccggggat catcgaaggc gcggcggagg gaaaggggct ggggcaccgg ttcctgcggc
     1021 atatcgagcg aaacccggtg ttgttgttcc tgatccctgc cgacagtcct aatcattccg
     1081 aggaattccg catccttgtc catgagctgg agaaatacaa tcccgagctg ctgcacaagc
     1141 ggtttatcat tgcgatcagt aaaagcgata tgctggatga tgagctgaag gaagcgatca
     1201 cccgggagct accccccgat attccccatc tttttatctc ttcgatgatc aatcagggga
     1261 tcctcgaact taaggacctg ctttggaaag tcctaaatga ggacgttaca aatagtaata
     1321 aataaaatac tactctctta acattaaatt catatagaag tattggaatt aatcaatttt
     1381 ggacatatat ttgctgtata caatatgaga tcgtcctgat ctcttcttcg tccagcctgc
     1441 cattcgattg ttgctgtcga cgattgttct tcccgccacc gtctaaccgg cccgccgcct
     1501 gccgccaact ttctttccgc ttgtcatcgg ttgactttcc atactaatag ggttgcccca
     1561 tatccttttc gtcactaaaa cccatttata tgaagtcgct attcatcatg cgctttggca
     1621 agcttctcct cggggcctct tttctgttga tcctggcaac ggcccatggc cagtccctgg
     1681 tttcctccag tggttcaggc gattccgccg tcacggtaag gtatcttggc gtgcaggacg
     1741 atctgctcgt ttttaatgtc gcgtattcca acccggatgg gagccggttc cagctcacgg
     1801 taaaagacca ggacggagcg cagctttacc agagtatctt taacgacaag gcctttttca
     1861 agcagttccg tctgccgaaa tccgacaagg accggatggt ctttatcttt catgattaca
     1921 aggacgcgga tatcgtaaag gcctttgacg tgaatgtcaa cagccgtttt gtccgggaag
     1981 tcgccgtcag gaaggtcgac taaccgttct tttcaccact atttccaaac acttaaaaat
     2041 actttttatg aaatcgatcg ttctctccct tctttttgcc ggcgtcagcc tggtaagcct
     2101 ggccaacggc catgatccta tcggtgataa ggccgcgagc actgcggaag taaagtatat
     2161 caccgcgggc aatggggcat tctttttcaa tgtgacctat aacaatgcca ccggcggccg
     2221 ctttagtgtg gtcgtgctgg atgagtatgg caaccagttg taccagaacg tctataccga
     2281 caagaagttc gacaagaagt tcatgctggc cgatcccgat aatttcaaga agctgacctt
     2341 tgtcatccgc aattacagtg acaataccgt tcagcgtttt gaggtgaatt cggatgatcg
     2401 catggtagag gatgtagaag taaaggaagt tcagtaagaa gatcatcgat atccggccgc
     2461 ccctgcgggg cggccggaag agggaaataa gctataattc tatcgaccct gtaccggggt
     2521 acttgccgct ttttgaatat ccgtaaatgt cattttgctg agctgttgct gggaaaaatt
     2581 cttcagcgag gtggatgcca gcacgttgcc ggggatcacc acatagcgat acaacagcga
     2641 agagttggag gtgctgaaat cgttgagata gccaaaggat tcgatctcga tgttccccac
     2701 gttcaacagt tggtacagcc catagtcgga tgccggggcg accgtcgtat ctccactggc
     2761 aggagaaccc aggtagctca atactgcgcc cgagcggatg accgccgcgg tgaccgtttt
     2821 tgccgtgata tcctggtagt aggcggtatc accggcgtcg gtggccttca tggaaatggt
     2881 cagccaggga gagtaataca cactatcctt taccacattc gtagtggtct ttttacagcc
     2941 gacaacggag aaaagagatg agagggatcc gatcacgata agggcggaaa gaacaatttt
     3001 caatttactc acaatattgc attttaaggt taacgatgat atgcgatcca tatagcaata
     3061 cgcaatccaa tagagtggcg tctagggaaa atcggcaaat ttgaacaatt taccggttaa
     3121 ttgactaaag atactacttt ttcttggccg cgatcgccgg cctgttatct ttccaacgcc
     3181 ataggtgcat acacgcatag gtgcggtacg ggctccattt tgccgagatg cgcagcaggt
     3241 cttcccggaa ggctttttta tccgaccggt ccaggttata gaggtggatc atggcgttct
     3301 ggacacccca atcgtctatc gcaaagacgt cctccctgcc gaggctgaac atcagcaaca
     3361 tttccgccgt ccatctgcca acccctttta tctgtgtcag ccaggcgatc acctcctcgt
     3421 tgtccatcct gcccagcttc ttgtgatcca ttcccatttc catggcgaat tgcgccacgt
     3481 tttgcacgta ccgggccttt gcattggaca ggccgataga ccgaagggtc tcgaaggggg
     3541 tggccagtac ttcttcgggt gtggggaccc ggtctccgta cagggccagg aaccgctgcc
     3601 ggatggtgtt ggcgaccttt gtagataact gctggctcat gatggaggcg atcaggtaaa
     3661 agtggatatc cttttgcttt ttgatgcgca cgggctcatg atctccgatg agttttttca
     3721 gttttttatc tttcgagaga tggagcaggt attccatgga aagcttgtcg tttattcatc
     3781 aggcaaaggt gtaactcact cccccatcct tctcatcttt taagacgatg cccagttgca
     3841 gcaactggtt gcggatcttg tcggaagtgg agtagtcttt ccgggagcgg gcgtcgcggc
     3901 ggatgtcgat gagcagttcc attacaccgc tcagcttttg atcgtcgcca agggattccc
     3961 ctttcaaacc gaggatattc tcgagatagt cggtaaaata gcgtttcatc agctgaaggg
     4021 tagcggggct cagggcgtcc cggcggatct gaccgccctt cagggagttg acgatgggga
     4081 cgagctcgaa gagattggcc agcacctttg cggtgttgaa gtcgtcgttc atgaattcgt
     4141 cgagttcgtg cagcaatttg cgaacgttgt catcgatgga cggatcggcc gccgttgccg
     4201 ggctgccgtt gcctccggcg tcatcggccg ccagtttttt tagcaggtcg taggcttccc
     4261 agaggcgttt gagccctttt tccgcggcct gcagtgcttc gttactgaag tcgagggtgc
     4321 tccggtagtg tgtctgcagg atgaagaagc ggatggtcat cggcgtgtag gcctgttgca
     4381 agagggggtg ggtgccggtg aacatctccg tgagccggat ctggttcccg tagctttttc
     4441 ccatcttttt cccgttgagg gtgatcatgt tgttgtggag ccagtaccgg gcggggatct
     4501 ccttgttgca gatggtgctt tgggcgatct cgctttcgtg gtgggggaac tggaggtcca
     4561 ttccaccgcc gtggatgtcg aactgggcgc cgagatattt tgagctcatc gcggagcact
     4621 cgatatgcca gcccgggaag ccttcgcccc aggggctttg ccagcgcatg atatgttccg
     4681 gcggggcggc cttccaaagg gcgaaatcct ggcggttgtg tttttcgtcc tggccgtcga
     4741 ggtcgcgggt cgtctccagg agatcgtcga gcactctgcc gctcaattta ccataggggt
     4801 agtgtgcggc atatttcttt acgtcgaaat agacggagcc gttgatctca taggcatagc
     4861 cttccctgat gatcgttttg atcatgctga tctgttcgac gatatggccg gtggccgtgg
     4921 gttcgatgct ggggttgagg ttgttgaact gccgcatggc ccaatggaag agattggtgt
     4981 atttctgcac cagctccatg ggttccagtt tttcgagcac ggcttttttg gcgatcttat
     5041 cctctgcctc gcggccttct tcttcgaagt gtccggcatc ggtgatattg cgaacatacc
     5101 tgaccttgta gccgaggtgg agcaggtagc gatagataaa atcgaaggtg atcgtggggc
     5161 gggcgtgacc aaggtgactc tcgccgctga cggtgggtcc gcatacgtac atgcctacat
     5221 gaccgggggt gatgggcgtg aatacctctt tctggcgggt gtaagaatta tggactttta
     5281 actgactcat acggcccgaa gatacgtcag tttcccaatt tcacatcggc ta
//
LOCUS       DATGFG010000704         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_649055, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000704 DATGFG010000000
VERSION     DATGFG010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649055"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VKQ52_13155"
     CDS             <1..400
                     /locus_tag="VKQ52_13155"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HLZ88193.1"
                     /translation="ANETIRRFFHLDPDRKILKLSKLKGEESGEPMVYFESYFHPRIG
                     VGEKDDFDRPLYSMLEERYGVVVHRSSENISARTAGALGKKLKVDSKFPVLFRERFVY
                     DVGDRPVEYNIGYYRSDKFTYSIDIKKGSL"
     gene            452..>2167
                     /locus_tag="VKQ52_13160"
     CDS             452..>2167
                     /locus_tag="VKQ52_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLZ88194.1"
                     /translation="MFKHTNNTNLRLGADIGGTHISAALIADGVLLPETYRRVAVDPN
                     TDASTLLDDWAGLLNAVLYLASPEQVKGIGLAMPGPFDYPNGTSLIRGLNKYESLFGI
                     NIKEALRDRLTADLRDRVTTTDLRNHIDPRPDDIGQELPISFENDAACFGLGEARSGQ
                     ATGYRKIIAITLGTGLGACFIDEKNIVTTGEGVPPNGYLYCIPFESGIAEDYLAARWL
                     LSHYPAADVKEIAQKARVYKDQRAIAAFSQFGANLGRFLAPWIRAFNADALVIGGSIA
                     QSYDLFGPAMQMEVPVVVSIHTEHMAIAGAASLVEEDKPTKPARKSLQPMLPKRVAPH
                     ADGPGSPPHAQNGNQDSQVAGASDAYHLYPFEPLGDGRIFSGYDALAQWIIGQKGIRI
                     DGYAGIDWNLVRSRLCPALRKLGLRVLWYETQAFLKPPAEIEALVKPFLGQEGSVWGT
                     KTTLSLSDLFTDDLRNWQPGAATAPHATRHSSTSSMLTATDTRTPPNTLIILAGTGAG
                     LSHWNAPIVYFDLPKNELQYRMRAGSATNLGSTTTGPFAEMYKRSYFVDWIVLNKHRR
                     AISDSI"
BASE COUNT          471 a          715 c          572 g          409 t
ORIGIN      
        1 ggccaacgag acgatccgcc ggttcttcca tctcgatccc gacaggaaga tcctcaagct
       61 atccaagcta aagggcgaag agtccgggga gcccatggtc tacttcgaga gctatttcca
      121 tccccgcatc ggcgtcggcg aaaaagatga cttcgatcgc cccctgtact ccatgctcga
      181 agagcgctat ggggtagtgg tgcaccgcag ttcggagaat atcagcgcgc ggacggccgg
      241 ggcgctgggg aagaagctaa aggtcgattc gaaattcccc gtgttgttcc gggagcggtt
      301 tgtgtatgat gtcggtgacc gaccggtgga gtacaacatc ggttattacc gcagcgataa
      361 attcacctat agtatcgata tcaaaaaagg aagtctgtag tctatatccg tacggccccc
      421 gggcccccat cattttttgc cttgccgaat tatgttcaaa catactaaca ataccaacct
      481 gcggctcgga gccgatatcg gcggcacgca tatctccgcg gcgctgatcg cagatggcgt
      541 cctgctgccg gaaacctacc gccgggtggc tgtagatccc aacaccgatg ccagcacctt
      601 gctcgacgac tgggctggcc tgctgaatgc cgtgctctac ctcgcatccc cggaacaagt
      661 taaaggcatc ggtttggcga tgcccggtcc gttcgactat cccaacggca cttccctgat
      721 caggggtctc aataaatacg aaagcctttt cggtatcaat ataaaggaag ccctccgcga
      781 ccggctgacc gccgacctcc gcgaccgggt aaccaccacc gacctccgca accacatcga
      841 cccaagaccc gatgacatcg ggcaggaact ccctatctcc ttcgaaaatg acgccgcctg
      901 ttttgggctc ggcgaagccc gctccggcca ggcgacgggc tatcgcaaga tcatcgccat
      961 caccctgggc accggtctcg gcgcctgctt catcgatgaa aagaacatcg tcacgacagg
     1021 cgaaggtgtc ccgccgaacg gctatctcta ctgtatcccg ttcgaatccg gcatcgccga
     1081 agactatctc gccgcccgct ggctgctatc ccattaccct gcggccgacg taaaggagat
     1141 cgctcaaaaa gcccgcgtat acaaggatca gcgggccatt gctgcgttca gccagttcgg
     1201 cgccaacctg ggccgtttcc ttgctccctg gataagggcg ttcaatgcgg acgcgctcgt
     1261 catcggcggg agtatcgccc aatcctatga tctctttggt ccggccatgc aaatggaggt
     1321 ccccgtagtc gtatcaatcc atacagaaca catggccatc gccggggccg ccagcctggt
     1381 ggaagaagat aagccgacga agccggcgcg gaaatcgctc cagccgatgt tgccaaagcg
     1441 cgtagctccg catgccgatg gccccggcag tcccccgcat gcccagaacg gcaaccagga
     1501 ctctcaggtc gcaggcgcct ccgacgccta ccatctctat cctttcgagc ccctcggcga
     1561 tggccgcatc ttctccggct atgatgcgct ggcgcagtgg atcattggcc aaaaaggcat
     1621 acgaatcgac ggttacgccg gcatcgactg gaacctggtg cgcagccggc tctgccccgc
     1681 gttacgaaag ctgggcctgc gcgtgctttg gtatgagacc caggccttcc tgaaaccgcc
     1741 agcagaaatc gaggccctgg tgaagccgtt ccttggccag gagggttcgg tatggggaac
     1801 aaagaccacg ttaagcctaa gcgatctctt caccgatgat ctgcgcaact ggcaacccgg
     1861 ggcggcaaca gctccccacg ccaccaggca ctcaagcacc tcctccatgc tcacggccac
     1921 cgacacccgc accccaccaa acaccctcat cattctcgcc ggtacgggcg ccggcctcag
     1981 ccactggaac gcccccatcg tctacttcga tctccccaag aacgagcttc aataccggat
     2041 gcgcgccggc agcgctacca acctgggctc cactaccact ggccctttcg cggaaatgta
     2101 taagcgcagc tacttcgtcg actggatagt cttaaacaaa caccgccgcg cgatcagcga
     2161 ctccatc
//
LOCUS       DATGFG010000705         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_179407, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000705 DATGFG010000000
VERSION     DATGFG010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179407"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..666
                     /locus_tag="VKQ52_13165"
     CDS             73..666
                     /locus_tag="VKQ52_13165"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HLZ88195.1"
                     /translation="MIKTGNPVISIYTEMTPNPETMKFVANKLLYPGKIIDFPDVESA
                     RPSPLAIELFGFPFIKSVFIASNFVTLTKTPDTDWDDVIPAIRQFLKEYLEEGKPVIN
                     EEDVVVVKSEGNAIDADDDDVVKRIKELLENYVKPAVEMDGGAIQFKSYNEGVVNLML
                     QGSCSGCPSSMITLKAGIESMMKRMIPEVKEVVAEAE"
     gene            666..1199
                     /locus_tag="VKQ52_13170"
     CDS             666..1199
                     /locus_tag="VKQ52_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88196.1"
                     /translation="MLKIVIVGPESTGKSSLCEELARHYQTDWVPEYAREYLTRHGMD
                     YRREDLLVIAREQLALEDRYAAAALAAKKKLLFIDTDMYVMKVWSEFVFGNCDPWILG
                     QIAQREYDAYLLCRTDLPWAADPLREYPDLASRDRLFHIYKDCLVNQSTPWAEIGGQG
                     EDRVAAGVAAVERLLLQ"
     gene            complement(1188..1889)
                     /gene="mtgA"
                     /locus_tag="VKQ52_13175"
     CDS             complement(1188..1889)
                     /gene="mtgA"
                     /locus_tag="VKQ52_13175"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HLZ88197.1"
                     /translation="MAINTKGLIPRIWRIIKRVVIFLFVFQLFYLLLLKWVDPPITLT
                     QLGSVFRGEGLKRSYVSMKDISPYAKLAVICSEDQLFPDHDGFDFKSIEKAMKHNQKS
                     KSLHGASTISQQVAKNVFLWQGRSWFRKALEAYFTFMIEWMWGKKRILEMYLNISEMG
                     KGIYGIEAAAHNYFNKPARNLTRLEAARIAACLPNPKVFTIHPLSSHVATRSTWIIGQ
                     MGVLQNDPDIQKLLQ"
     gene            complement(1917..>2248)
                     /locus_tag="VKQ52_13180"
     CDS             complement(1917..>2248)
                     /locus_tag="VKQ52_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88198.1"
                     /translation="NIDDLRRDTVYINGQAFSYKEIGAIKRERTKLSNRGTGILLTTA
                     GAGLFAIGAINGANRGDAAKQWYTTSGYIVGGALIATGVILIATAKKYYPLGGRYKLQ
                     YLQIGRR"
BASE COUNT          488 a          591 c          636 g          533 t
ORIGIN      
        1 caggaatttc ccgattcggc aaccaggcgt gacgccaatt cgttaacttt gtacctccta
       61 aatagcgctc ttatgatcaa aacaggaaac ccggtgatca gtatctatac cgaaatgact
      121 ccgaacccgg aaacgatgaa gttcgtggcc aacaagttgt tgtatcccgg taagatcatc
      181 gatttcccgg atgtcgaatc cgccaggcct tctccgctgg ccatcgagct gtttggtttc
      241 ccctttatca agagcgtttt tatcgccagc aactttgtta ctctcaccaa gactcccgat
      301 acggactggg acgatgtcat tcccgccatc cgccagttcc tgaaagaata cctcgaggaa
      361 ggtaaacccg tgatcaacga ggaggacgtg gtcgtcgtca agtccgaagg caatgccatc
      421 gacgccgatg atgacgatgt cgtaaaacgc atcaaggagt tgctcgagaa ctatgtaaag
      481 cctgccgtgg aaatggacgg tggcgccatc cagttcaaga gctacaatga aggcgtcgtc
      541 aacctgatgc tacagggctc ctgctcgggt tgtccctctt ccatgatcac gctgaaggcc
      601 ggcatcgaaa gcatgatgaa gcgtatgatc cccgaagtaa aagaggtcgt tgccgaagcc
      661 gagtaatgct gaaaattgtc atcgtcggac cggaatcgac cgggaagtct tcgctttgcg
      721 aggagcttgc ccgtcattac caaacggatt gggtacccga atatgcccgg gaatacctga
      781 ccaggcatgg aatggattac cggcgggagg acctgctggt cattgcccgg gagcagcttg
      841 cgctggagga ccggtatgca gcggcagctc ttgccgcgaa aaagaagctc cttttcatcg
      901 acaccgatat gtatgtgatg aaggtctgga gcgaatttgt ctttggaaat tgcgatccct
      961 ggatccttgg gcagatcgcg cagcgcgagt atgacgctta tcttctttgc cgtacggacc
     1021 ttccctgggc ggccgaccct ttgcgggagt atcccgatct ggcctcgagg gaccggcttt
     1081 ttcatatcta caaggactgc ctggtcaacc agtcaacgcc ctgggccgag atcggggggc
     1141 agggggagga tcgggtcgcc gccggtgtcg ccgccgtgga gcgcctttta ttgcaatagt
     1201 ttctggatat cggggtcgtt ctgcaggacc cccatctggc cgatgatcca ggtgctgcgg
     1261 gttgccacat ggctggagag cggatggatg gtaaagacct ttgggttggg caggcaggcg
     1321 gcgatgcggg cagcctcgag ccgggtcagg ttacgggctg gtttgttgaa atagttatgc
     1381 gccgcggctt cgatgccata gatccccttc cccatctcgc ttatgttgag atacatttcg
     1441 aggatgcgtt tcttccccca catccattcg atcataaagg taaaataggc ttccagcgcc
     1501 ttccggaacc agctccggcc ctgccagaga aagacgttct ttgccacctg ctggctgatg
     1561 gtgctggcgc catgcagcga cttgctcttc tggttgtgtt tcatcgcctt ttcgatggac
     1621 ttgaaatcga agccgtcatg atcggggaag agctggtctt cggagcagat gaccgccagc
     1681 ttggcgtagg gcgagatatc cttcatcgat acatagctgc gtttgagtcc ttcgcccctg
     1741 aatacgctgc ccagttgggt cagagtgatg ggtggatcga cccatttcag gaggagaaga
     1801 taaaataact ggaagacgaa aaggaagatt acgacacgct tgatgatacg ccagatgcgg
     1861 gggatcagtc cttttgtatt gatggccatc cagaagattt acgcctgcaa tataccctag
     1921 cgccggccga tctgcaaata ttgcaacttg tatctcccgc caaggggata atattttttt
     1981 gccgtcgcta tcaggatgac gccggtagcg atgagggctc cgcccacgat ataacccgaa
     2041 gtcgtatacc attgctttgc cgcgtcgccg cgattggcgc cgttgatggc accgatggcg
     2101 aataaacccg cgccggcggt cgtcagcagg atgcccgtgc cgcgattgct cagctttgtc
     2161 ctttctcttt ttatcgcgcc gatctcctta tagctaaagg cctgtccgtt gatatagacg
     2221 gtatcccggc gaaggtcgtc gatgttgc
//
LOCUS       DATGFG010000706         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1380530, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000706 DATGFG010000000
VERSION     DATGFG010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380530"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..520)
                     /locus_tag="VKQ52_13185"
     CDS             complement(119..520)
                     /locus_tag="VKQ52_13185"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HLZ88199.1"
                     /translation="MKRRKFLSASAMTAGVIGLGGEAGATKLPPVVHHVFFWLKNAGS
                     VEDRDKLVEGLKTLAGIPLIKELHVGVLADTEKRDVVDASWQVSELMFFDNVNDQASY
                     QQHPLHLAFIKNYSHLWAKVVVYDAMNVYSR"
     gene            939..2114
                     /locus_tag="VKQ52_13190"
     CDS             939..2114
                     /locus_tag="VKQ52_13190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ88200.1"
                     /translation="MRKALPVLLILSALTATTVNAQTIVSEFLWNSGSATHADVGSNA
                     TLGPGSTAITAAGGVGGTTGVEPNGNNINLQIPGAEFELNGLDISEDFLRKENGASFF
                     TLGGMDFGINTGAIYAKFLLNKSGTDTAVSLANFMTVPTDANFHTYRFVYDNVSGTFF
                     AFLDGTLEYNKKLSTGYPLSWTGATNATIGSGMDGSGAAFPILDNFIAQTPLVVLPVQ
                     LLSFDATTADGANQLEWTTADPGHLRDFTIERSANGTDFTAIGALDAQPGNNYHYTDN
                     NPPATSFYRLRMTDADGTSTWSPVRKLSIATDISISCYPNPVVNYASIRLNNTAPETW
                     RLTVATLDGRLIRTGELEAASAGQQFNLDLTTAPKGILLISLQTANHATRTFKILKQ"
     gene            complement(2115..>2647)
                     /locus_tag="VKQ52_13195"
     CDS             complement(2115..>2647)
                     /locus_tag="VKQ52_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88201.1"
                     /translation="DGGGKDNSGAGTSAAIFFALSRYIGEELAAGRDRGYDSLLRKLR
                     IYFVSISNSATSTLHIPPADDRRVVHNRFEPLNPVVGIMNSGVNGNAMEADSALRCRF
                     RGNPMFGSLYGGYFSVWPNTFCINAGTDSAYCPLAPLGWQISLPSLRRLEVNFTPEKI
                     GGNPEGIPKILGVVPR"
BASE COUNT          598 a         1003 c          551 g          495 t
ORIGIN      
        1 gccgcatctc gcctatggtg atcgccaggt cagcgccgag atcggaccca acagcaccct
       61 gatcttcgat gtcgaattga tcagctttac ctaacggctc tgcgggccgc ccgacggtct
      121 atctggaata cacattcatc gcatcataca ccaccacctt cgcccacaga tggctataat
      181 tcttgatgaa ggcaaggtgc aggggatgtt gttggtacga agcctggtcg ttgacattgt
      241 cgaagaacat cagctccgac acctgccagg aagcatcgac aacatccctc ttttccgtat
      301 cggccaacac ccccacatgc aactccttga tcagcgggat ccccgccagc gtcttcagac
      361 cctccaccag cttatcgcga tcttccacag accccgcatt cttcagccag aaaaaaacat
      421 gatgcaccac cggcggcaac ttcgtcgccc ccgcctcacc tcccaaaccg atcacccccg
      481 ccgtcatggc cgaagcactg agaaactttc ttcttttcat atgtcctgtt ttatcggcaa
      541 tataatcgcc atttccttcc caaaaaacta ataaagggcg ttccccgccg ccgcgccaaa
      601 aatccgcggc cgcgcctcgt ccctcggcgc tcgcaaactc gtccctcgtt tgccggcggc
      661 gggccgggct ctccactcca agtcctcgcc ctcccagacc ttcgctgacg ctcggctatc
      721 tcggtcgggc tccgggcttt ccgttccgat ccctaacgcg gcccccggcc accccaaccc
      781 gcgaccctct tcgtcccaca ttcgtcccct tttcccaaat ctggaaaacc cttccgcgtc
      841 ctggaaagcg ataacgtact acgtagtttt caatcgactg ataaccaagt cataacgcct
      901 tggcatcggg attgctccca gagggcaaat cctattccat gcgcaaagcc ttacccgtcc
      961 tcctgatctt atccgccctg acggcgacta ccgtcaatgc ccaaaccatt gtcagcgaat
     1021 tcctctggaa ttccggctcc gccacccacg cagacgtggg cagcaatgcc accctcggcc
     1081 cgggctccac cgccataaca gcagccggcg gcgtaggcgg caccaccggc gttgaaccca
     1141 acggcaacaa tatcaatctc cagattccgg gcgccgaatt cgaactcaac ggcctcgata
     1201 tctcggaaga cttcttaaga aaggagaacg gcgccagctt cttcaccctc ggcggaatgg
     1261 acttcggcat caataccggc gctatctatg cgaaattcct gttgaataaa agcggcaccg
     1321 acaccgccgt ctccctcgcc aacttcatga ccgtcccgac ggacgccaac ttccacacct
     1381 atcgcttcgt ctacgacaac gtctcgggaa ccttcttcgc cttcctcgac ggcaccctcg
     1441 aatacaacaa aaaactcagc accggctatc ccctgtcctg gaccggcgcc accaacgcca
     1501 ccatcggttc cggcatggac ggcagcggcg cggccttccc catcctcgac aacttcatcg
     1561 cccaaacgcc cctcgtcgta ctcccggtgc aattgctctc cttcgacgcg acaacggcag
     1621 acggcgccaa ccagctcgaa tggacaaccg ccgatcccgg ccatctccgc gacttcacca
     1681 tcgaacgctc cgccaacggc acggacttca ccgccatcgg cgccctcgat gcacagccag
     1741 gcaacaacta tcattatacg gataacaatc cccctgccac cagcttctac cggctccgca
     1801 tgaccgatgc cgacgggaca tccacctggt cgcccgtgcg aaagctgagc atcgcaacgg
     1861 atatatcgat ctcctgttac cccaacccgg tcgtcaatta tgccagcatc cggctcaaca
     1921 acactgctcc ggaaacctgg cgcctcaccg tcgcaaccct cgacggccgc ctcatccgca
     1981 ccggcgaact cgaggccgca tccgccggcc agcagttcaa cctcgacctc accaccgcgc
     2041 caaaaggcat cctcctcatc agcctccaaa ccgccaacca cgcaactcgg acattcaaga
     2101 tcctaaaaca ataattaccg cggcaccacc cccaatatct tcggtatccc ctccgggttc
     2161 cccccgatct tctccggcgt aaaattcacc tccagccgcc tcagcgacgg caacgagatc
     2221 tgccacccca acggcgccaa cggacaatac gcactatccg tccctgcatt gatacaaaac
     2281 gtattcggcc acaccgaaaa atacccccca tacagggagc cgaacatagg atttcctctg
     2341 aagcggcatc gcaacgcact atccgcctcc atcgcattcc cattcacccc gctgttcatg
     2401 atccccacga cgggattcaa cggctcgaac cggttgtgca ccaccctccg gtcatccgcc
     2461 ggcggaatat gcagggtact cgtcgccgaa ttggagatac tcacaaaata gatccgcaac
     2521 ttacgcagca gcgaatcata cccccggtcg cgccccgccg ccaactcctc accgatatac
     2581 cggctcaaag caaaaaatat cgccgccgac gttcccgccc cggaattatc cttcccgccc
     2641 ccatcca
//
LOCUS       DATGFG010000707         4719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1662970, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000707 DATGFG010000000
VERSION     DATGFG010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662970"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VKQ52_13200"
     CDS             <1..269
                     /locus_tag="VKQ52_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ88202.1"
                     /translation="VDEAVIYAISTRLRPKLMTALGAIIALMPLALGIGAGAQLHQPL
                     AIAVIGGFVIALPLLLIVLPSLLRWLYYRRWQRLNSRGALTRIQ"
     gene            complement(271..1377)
                     /locus_tag="VKQ52_13205"
     CDS             complement(271..1377)
                     /locus_tag="VKQ52_13205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ88203.1"
                     /translation="MKPTLILATLKGFPLYAGFLLPFLSPSPANAQCGGCSYTETELT
                     SGAMDVIPAGTIVCITTNTCLGATSAYPATCPYTGSGHLTINGILRICPGVTFSFDGT
                     ITGSGTIQIESTGRMNLYGTYDCNVHMTAVDPDIATGTSTSTTVGGCNSSACEPHFAD
                     GYAPFGVVATGLGYTVSSGTCAITGVPGNYVLPVQLLGWEAAWQGSAIRLSWSTPGKD
                     QGAFFEVDYSTSGAGWQPLSPKIPGIDNSPDARYSFLATGPLSAHNFFRLRYEDVAGQ
                     DSYSPIQELDAGGENPDGFFAGPNPLRQTLLVRTPAPGAYTLQLLDVTGKTLLRRSSS
                     PSGEYDCSGLAPGTYFLLITTQEGRRCMKKLVKD"
     gene            1461..1988
                     /locus_tag="VKQ52_13210"
     CDS             1461..1988
                     /locus_tag="VKQ52_13210"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ88204.1"
                     /translation="MEQTMIRIREAGPQDAALVADIARRTFYDSFAEHNTAENMRIFL
                     EEQWPREKQMAEVGAPGRFFLLAYVEDEPAGYVSLRVGEPPVGLQGANAIELVQLYSE
                     KKMIGKGVGPALMEAGLELARRMGHEWIWLGVWENNHRAQAFYRKWGFERFGEHVFFV
                     GLDAQTDWWMRKKLV"
     gene            complement(2062..2712)
                     /locus_tag="VKQ52_13215"
     CDS             complement(2062..2712)
                     /locus_tag="VKQ52_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88205.1"
                     /translation="MRKFYFSGLISCSLLLAAAQTNAQSFEQNGDPVMVSSPNHTGLL
                     AYAENNIIIIRWTSANERNIDRYIIERSDDSIHFTTLHELLSKGPVTTDAANTYEDAD
                     SYPATSTNYYRLRTVLRDGGAFYSSVVKVDIDARDLPVLKPTVLHMGGTLRLDNYRNQ
                     PVTVNIFNANGTLLGSWMVNSTSFNINTDRLARGILFYRISDETHPLIDAGKLMIQ"
     gene            complement(2857..3570)
                     /locus_tag="VKQ52_13220"
     CDS             complement(2857..3570)
                     /locus_tag="VKQ52_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HLZ88206.1"
                     /translation="MPTRIKCIITDDEPFARKGLQGYIDRIDFLQLQGICENAIELNS
                     LIKKEPVDLLFLDIEMPYLTGIDFLKQLATPPRVIFTTAYEKYALQGFELEVLDYLLK
                     PISFDRFLKAANKAFDYFRQQQTPAATEDYIFVKADSRLEKIAFADILFAEALENYVA
                     IYTREKKIITHLTLKMLQEKLPAASFVQPHKSYLVAIDKINSIEGNILHITQFQVPIS
                     KYQKEEIMEKIVNSKLLKR"
     gene            complement(3574..4605)
                     /locus_tag="VKQ52_13225"
     CDS             complement(3574..4605)
                     /locus_tag="VKQ52_13225"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ88207.1"
                     /translation="MKFFWKYIFPPLYGLFFYTILRLVSDLTTEDKFWERRIEQNLVE
                     VLGTIILSYPMVRLPGYFDRKFQKKKRPNNFRTISLEFGEILLVAMGMATGTSSLIHV
                     LAHDYPIGIDDLVIVNVLLSLFMLLHYAILRGNSFVQALIEQRVLTEKIRNDHLHTEL
                     KFLKAQYHPHFLFNALNTIYFQMDEDVDAAKRSVEKFSELLRYQLYDQQQTVPVNQEI
                     HYLDNFIRLQQTRSSEKLRLETFFDPHLDGQQVYPLLFLPLVENAFKYVGGEYQISIH
                     AALENGNIKFGVTNSIPANIPEHNRADKGIGLENLRRRLELLYPDKHTLIAEKTDDRF
                     RAALTLQYT"
BASE COUNT          893 a         1042 c         1699 g         1085 t
ORIGIN      
        1 cagtggacga ggcggtgatc tatgccatct ccacgcgtct ccggccgaaa ctgatgacgg
       61 ccctgggggc cattatcgcc ctgatgccgc tggcgttggg gatcggtgcg ggtgcgcagt
      121 tgcatcagcc gctggccatt gcggtgatcg gtggttttgt gatcgcgctg ccgctgttgc
      181 tgatcgtgct gccttcgctt ttgcgctggc tgtattaccg gcgctggcag cgactgaaca
      241 gccggggggc gctgacccgt atccagtagg ctagtctttt accagttttt tcatgcaccg
      301 gcggccttcc tgtgtggtga tgaggaggaa gtaggtgccg ggagcgaggc cggagcaatc
      361 gtattctccg ctggggctgg acgaccggcg aaggagggtt ttgcccgtga catcgagtag
      421 ctgcagggtg taggccccgg gtgccggtgt cctgaccagg agtgtctggc ggagggggtt
      481 ggggccggcg aagaagccgt cggggttttc gccgccggcg tcgagttcct gtatggggct
      541 ataggaatcc tggccggcga cgtcttcgta gcgcaggcgg aagaaattat gggcggatag
      601 cgggccggtg gcaagaaagg aatagcgggc gtccgggctg ttgtcgatgc cggggatctt
      661 tggtgaaagg ggttgccagc cggcgccgct ggtggaatag tcgacttcga agaaagcgcc
      721 ctggtcttta cccggcgtcg accagctgag acggatggcg gagccctgcc aggcggcttc
      781 ccagccgagc aactggaccg ggaggacata attgccgggg acacccgtta tggcgcaggt
      841 tccgctgctt accgtatacc cgaggccggt agccacgacg ccgaagggag cgtagccgtc
      901 cgcaaaatgg ggttcgcagg cgctgctgtt acagccgccg acggtggtgg aggtggaggt
      961 gccggtggcg atatcggggt cgacggcggt catatggacg ttgcagtcgt aggttccgta
     1021 aaggttcatg cggccggtgc tttcgatctg gatcgtaccg ctgccggtga tggtaccgtc
     1081 gaagctgaag gtaacgccgg ggcagatgcg caggatgccg ttgatggtca ggtgacccga
     1141 gccggtgtag gggcaggtgg cgggataggc ggatgtggcc ccgaggcagg tgttggtggt
     1201 gatacagacg atcgtgccgg cggggatgac atccattgca ccggaggtga gttcggtctc
     1261 agtgtaggag cagccgccgc actgggcgtt ggccggggag ggagacagga agggaaggag
     1321 gaagccggcg tataggggga agcccttaag ggtggccagg atcagggtcg gtttcataag
     1381 acggtttatt gataaaggtt tattgcggtg caagataacc cgcgggggcc atttttgcaa
     1441 gaaagtagta gtttgcaggt atggagcaga caatgatacg gatacgtgag gcgggcccgc
     1501 aggatgcggc gctggtcgcc gatatcgcca ggcggacttt ttatgattct tttgcggagc
     1561 acaatacggc ggagaatatg cgcattttcc tggaggagca atggccgcgg gaaaagcaga
     1621 tggcggaagt gggagcgccg gggcggttct ttctcctggc gtatgtggag gatgagcccg
     1681 cgggatatgt gtcgctgcgg gtgggggaac cgcccgtggg cttgcagggg gcgaatgcca
     1741 tcgagctcgt gcaattgtat tccgagaaga agatgatcgg caagggggtg gggccggcac
     1801 tgatggaggc tggtctcgaa cttgcccggc ggatggggca tgaatggata tggcttgggg
     1861 tatgggaaaa caatcaccgg gcgcaggcct tttaccggaa atggggtttt gagcggtttg
     1921 gcgagcacgt cttttttgtc gggctggatg cccagacgga ttggtggatg aggaagaagc
     1981 tggtatgagc ggccgctgtc cgatcagccg gatagagcag tcgttgcccg acaggccgga
     2041 tggagccagg gcttttgccg gctactggat catcagcttc ccggcgtcga tgaggggatg
     2101 ggtctcatcg gagatgcggt agaagaggat gcctcttgcg aggcgatcgg tattgatgtt
     2161 aaaggaagtg ctgttcacca tccagctgcc caacagggtg ccgttggcat tgaagatgtt
     2221 gacggtgacg ggctggttgc gatagttatc caggcggagc gtaccgccca tgtggaggac
     2281 cgtgggtttc aggacgggca ggtccctggc gtcgatgtcc acctttacca cggatgaata
     2341 gaaggcgccg ccgtctctga ggacggttct cagccggtag tagttggtgg aggtggcggg
     2401 atagctgtcg gcgtcctcgt aggtgttggc cgcgtccgtg gtgacggggc ccttcgacag
     2461 gagttcgtgg agggtggtga agtggatgct gtcgtcactg cgctcgatga tatagcgatc
     2521 gatatttctt tcgttggcgc tggtccagcg gataatgatg atattatttt cggcgtaggc
     2581 gaggaggccg gtgtggttgg gtgagcttac catgacgggg tccccgttct gttcgaagga
     2641 ctgggcattg gtctgagcgg ccgccaggag gaggctgcag gagatcaggc ctgaaaagta
     2701 gaactttctc attttacctg atttttatag gctcgggccg ccgggggcag cccatgtaaa
     2761 gttacgtgca aacggcatgc ccgggatcgg ccgtcctgca tcgggggcat gattaacggg
     2821 gtattaacgc gtcaggtgag tcaacgctca agtgtgtcaa cgcttcagga gcttgctgtt
     2881 gacgatcttc tccatgatct cttctttctg gtatttgctg atgggcacct ggaactgggt
     2941 gatgtgaagg atattgcctt cgatggaatt gatcttgtcg atggcgacga gatacgattt
     3001 atgcggctga acgaaggatg cggccgggag tttttcctgc agcattttca gggtgagatg
     3061 ggtgatgatc tttttctcgc gggtgtagat ggccacgtaa ttctccaatg cctcggcgaa
     3121 gaggatgtct gcgaaggcga tcttctcgag ccggctgtcc gcctttacga agatatagtc
     3181 ttcggtggcg gccggggtct gttgctggcg gaagtaatcg aaggccttgt tggccgcttt
     3241 caggaagcgg tcgaaggaaa tgggtttgag gagatagtcg aggacttcca gttcgaagcc
     3301 ctgcagggca tatttctcat aggccgtggt aaaaatgacc cggggtgggg tggccagttg
     3361 tttgaggaag tcgatgccgg tcaggtaggg catttcgatg tcgaggaaga gcaggtcgac
     3421 gggttctttt tttataagcg agttcagttc gatggcgttc tcgcagatgc cctgcagttg
     3481 gaggaagtcg atgcgatcga tatacccttg cagtcccttg cgggcaaagg gttcgtcgtc
     3541 agtgatgatg cattttatcc gggtgggcat cggctatgta tattgaaggg ttagcgcggc
     3601 gcgaaaccgg tcgtccgttt tttccgcgat gagggtatgt ttatcggggt agagcagctc
     3661 caggcggcgg cggaggttct ccaggccgat gcctttatcg gcgcggttat gctcggggat
     3721 attggcaggg atgctgttgg tgacgccgaa tttgatatta ccgttctcca gtgcggcgtg
     3781 gatgctgatc tggtactcac cgccgacgta tttgaaggcg ttctccacca gcgggaggaa
     3841 gagcagggga tagacctgtt gtccatcgag gtggggatcg aagaaggttt cgagccggag
     3901 tttttcggag ctgcgggtct gttgcaggcg gataaaattg tcgagataat ggatctcctg
     3961 gttgaccggt acggtctgtt gctgatcgta gagctggtag cgcagcagtt cggagaattt
     4021 ctccacactt cgtttcgcgg cgtccacgtc ttcatccatc tggaagtaga tggtgtttag
     4081 tgcgttgaac agaaagtggg gatgatattg cgccttcagg aatttcagtt cggtatgcag
     4141 atggtcgttc cggatctttt cggtgagcac gcgctgttcg atgagggcct ggacaaagct
     4201 gttgccgcga aggatggcat aatgcaggag catgaagagc gagagcagga cgttgacgat
     4261 gacgaggtcg tcgatgccga tgggatagtc gtgggcaagg acgtggatga ggctgctcgt
     4321 gccggtggcc atgcccatgg ctacgagcag gatctcgccg aattcgaggg agatggtgcg
     4381 gaagttgttc gggcgttttt tcttttggaa tttgcgatcg aaataccccg gcaggcggac
     4441 catgggatag gacaggatga tcgtgcccag cacctccacc aggttctgct cgatgcggcg
     4501 ttcccagaac ttatcctccg tggtgaggtc ggacaccagg cgcaggatgg tgtagaagaa
     4561 cagcccatac agtggcggaa agatatattt ccagaagaac ttcatgtggg taagatacga
     4621 ttttacagca tgtttttcag tagcgggatg cggcggaaga tcccggcgca aagggcgtag
     4681 gtgagggtga tgctcaggat cgtggctatg aggaatttc
//
LOCUS       DATGFG010000708         3587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_583759, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000708 DATGFG010000000
VERSION     DATGFG010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583759"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VKQ52_13230"
     CDS             complement(<1..240)
                     /locus_tag="VKQ52_13230"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLZ88208.1"
                     /translation="MSNLNLNSDKQGLSPSDKEFENNIRPSVIDDFSGQPQLIENLRV
                     FIKAAKMRGEALDHILFHGPPGLGKTTLSRIVANEL"
     gene            308..949
                     /locus_tag="VKQ52_13235"
     CDS             308..949
                     /locus_tag="VKQ52_13235"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLZ88209.1"
                     /translation="MDIPGNSRKMETAYLEKAISYEAYIALIDELVAGRRTTGPDQSE
                     AFIHYTQLNQQRMHRWEKTIQLLPSAESAIRSIAAPQTWLVLTEAWCGDAAHIIPVLH
                     ALAALNPLITLCFLMRDENPELMDRYLTNGVSRSIPKLISLDTATREELFTWGPRPAP
                     LQKIFMKLREEGTAFAAIKEELQRWYNKDKSITIQEEMAALAIGTTGAIGMTA"
     gene            complement(1016..1996)
                     /gene="egtD"
                     /locus_tag="VKQ52_13240"
     CDS             complement(1016..1996)
                     /gene="egtD"
                     /locus_tag="VKQ52_13240"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HLZ88210.1"
                     /translation="MFPPSTANVAARRSTTFYTDVINGLRAEHKHLSSKYFYDEVGDK
                     LFQQIMACPEYYPTRCEMEILQHQSEKMARSFMEDSADFDLVELGPGDATKSWFLLRQ
                     LQRDRASFTYYPIDISSNVISWLEDKLPVTLPGVRLQGLNGEYLEKLHQVNTFSSKRK
                     VVLFMGANIGNMTVQQATQFCRQVHQQLRPGDLFLVGFDLKKHPRTILDAYNDRQGIT
                     RAFNLNLLTRINRELGADFDLGQFEHYPVYDPGTGACRSYLISRVEQEVHIGDKTIAF
                     AENEAIHMEISQKYTLVETESMAAQSGFEPVETFYDSRKWFADTLWRRLP"
     gene            complement(2171..3364)
                     /gene="egtB"
                     /locus_tag="VKQ52_13245"
     CDS             complement(2171..3364)
                     /gene="egtB"
                     /locus_tag="VKQ52_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HLZ88211.1"
                     /translation="MTLFDQYRAVRKASEDICRPLQKEDYVVQPVVDVSPPKWHLGHT
                     TWFWEAFVLIPHSPGYRPCNPDYNYVFNSYYETIGARVVRTDRGNLSRPSVDEIYTYR
                     QYVDAAMERLIAAGPAGELEKIIILGLNHEQQHQELLYTDIKYILGHNPLFPAWSDRD
                     IDEGPSADDGSGFVTSEGSSADKGSGFVTMPAGLYKIGYKGDGFCFDNELSRHKVWLD
                     SYRLAVSGVTNAEYLEFMEAGGYGNFVWWHSEGWDWVKKNAVSAPLYWHLIDGRWHRY
                     TMAGLKPLDGKQLLSHISYYEAVAYAAWKGCRLPTEFEWEAAAGQFRWGRRWEWTESA
                     YLPYPGFKKAEGAIGEYNGKFMVSQMVLRGASVATPAGHSRKSYRNFFHPHLRWQFTG
                     IRLAT"
     gene            complement(3364..>3587)
                     /locus_tag="VKQ52_13250"
     CDS             complement(3364..>3587)
                     /locus_tag="VKQ52_13250"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HLZ88212.1"
                     /translation="ILQPPSDLTARLSGSRDSVYRYVADRFDKEYRLTWLEPIGFNNA
                     YALMMRRSQMQQLGIRTISDLTNYLKTDQ"
BASE COUNT          783 a         1055 c          983 g          766 t
ORIGIN      
        1 cagctcgttg gccacgatcc gcgacagggt cgtttttcca agcccggggg ggccgtgaaa
       61 gaggatatgg tcgagcgcct cgccgcgcat ttttgcggcc ttgataaaga cacgcaggtt
      121 ctcgatcagc tggggctgac cggagaaatc atcgatgacg gatggccgga tattattctc
      181 aaactccttg tcagaggggc ttaagccttg tttatcactg ttgagattga gattggacat
      241 gtgtaaatac gctgattggg caggaacaaa tatacccgat atttgcagtg ccaaaaagga
      301 gaaatttatg gacatccctg gtaacagcag aaagatggag accgcctacc tcgaaaaagc
      361 catcagttat gaagcctata tcgcgctgat cgatgagctg gtagccggcc gccggaccac
      421 gggccccgat cagtcggaag ccttcatcca ctatacccaa ttgaatcagc aacggatgca
      481 ccgctgggag aaaacgatcc agctgctgcc ctccgcggaa tcggcgatcc gctcgattgc
      541 cgctccgcag acctggctgg tgctgaccga ggcctggtgc ggcgatgccg cgcacatcat
      601 tcccgttctt catgccctgg ccgcgctaaa cccgctgatc accctgtgct tcctcatgag
      661 ggacgagaac ccggagctga tggaccgcta tcttaccaat ggggtgagcc gcagcatccc
      721 caagctgatc agtctcgata cggccacgag ggaagaatta ttcacatggg gaccgcgccc
      781 cgcccccctg cagaagatct tcatgaaact aagagaagag ggtaccgcct tcgccgcaat
      841 aaaagaagaa ctgcagcgct ggtacaacaa ggataaatcc attaccatcc aggaagaaat
      901 ggcggcattg gccatcggga ccaccggcgc catcggcatg acagcctaga atggcgacgc
      961 atcacgcaca ccgccgacgc gcatcacgca cgccgcggac ccgtcgacgc atcgatcacg
     1021 gcaggcggcg ccacagcgta tcggcaaacc atttcctgct atcataaaaa gtctccaccg
     1081 gctcgaagcc cgactgggcg gccatcgatt ccgtttctac gagcgtatac ttctgtgaga
     1141 tctccatgtg gatggcttcg ttctccgcaa aggcgatggt cttgtcgccg atatgcactt
     1201 cctgttccac gcggctgatc aggtaactcc ggcaggcccc tgtccccgga tcatagacag
     1261 gataatgctc gaactggcca aggtcgaaat cggcgcccag ttcccggttg atgcgggtca
     1321 ggagattgag gttgaatgcc cgcgtgatcc cctggcgatc gttatacgca tcgaggatcg
     1381 tacgcggatg ttttttcaga tcgaaaccca ccaggaacag atcgcccggg cgcagttgtt
     1441 gatgcacctg ccggcagaac tgggtcgcct gctgtacggt catattaccg atgttggcgc
     1501 ccatgaacag gaccaccttc cgcttgctgg agaaggtatt cacctggtgc agtttctcca
     1561 ggtattcccc attgagcccc tgcagccgga cccccggcag cgttaccggc agcttgtctt
     1621 ccagccagct gatcacattg gaggaaatat caatcgggta ataggtgaac gaagcgcggt
     1681 ctcgctgcaa ctgccgcagc aggaaccagg attttgtcgc gtcgccgggc cccagttcca
     1741 ccaggtcgaa gtcggcgctg tcctccataa aggaccgggc cattttttcc gactgatgct
     1801 gcaggatctc catctcgcaa cgggtgggat aatactccgg acaggccatg atctgctgga
     1861 agagcttgtc ccccacttcg tcgtaaaaat atttcgacga caggtgcttg tgctcggccc
     1921 gtaacccatt gataacgtcg gtgtaaaagg tggtggaccg gcgtgcggcc acattggcgg
     1981 tgctgggagg aaacattaga ccttttttta ttagatattt atttggaatt cctgctgtgc
     2041 cggtctcgac acgccttcgg cgtggaatgt cggccgggga gaacttcgcc cttgccactg
     2101 gcaagagctc gttcgcatcc ttcgccttcg ctcggatgtt tcgtccttcg gccgacaggc
     2161 ttccagcctg tcaggttgca agccgtatgc cagtaaactg ccaccgcaga tggggatgaa
     2221 agaaattgcg gtaactcttc cggctatggc ccgccggggt cgccaccgac gcacctctca
     2281 gcaccatctg gctgaccatg aacttaccgt tatattctcc gatcgcccct tcggcctttt
     2341 tgaaccccgg gtagggcaga taggctgatt cggtccactc ccatcttctc ccccaccgga
     2401 actgccccgc agcggcctcc cattcaaatt ccgtcggtag acggcatcct ttccacgctg
     2461 cataggccac ggcttcgtaa taactgatat gagacaacaa ttgtttacca tccagcggct
     2521 ttaatccggc catcgtatac cgatgccacc ggccatcgat aagatgccag tacagcggcg
     2581 ccgaaacggc attcttcttt acccagtccc agccttccga atgccaccag acgaaattgc
     2641 catacccgcc cgcctccatg aactcgaggt actcggcatt ggtgaccccg gacacagcca
     2701 gccggtagct gtccagccag accttatgac ggctgagttc attgtcgaaa cagaagccat
     2761 cgcctttata gccgatctta tacaagcccg caggcatcgt cacgaaaccg gaacccttat
     2821 ccgcggagga tccctcactc gtcacgaagc cggagccgtc atccgcagac ggaccctcat
     2881 cgatatcccg gtcggaccag gcggggaaca gcggattatg acccaggatg tattttatgt
     2941 ccgtgtacag caattcctgg tgttgctgtt cgtggttgag cccgaggatg atgatcttct
     3001 ccagctcccc ggccggcccg gcggcgatca gccgttccat cgcagcatct acgtattggc
     3061 gataggtata gatctcgtcc acggaaggcc ggctcagatt accacgatcg gttcggacga
     3121 ccctcgcgcc gatagtttca tagtaactgt tgaagacata gttatagtcg gggttgcagg
     3181 gccggtaacc aggactgtgc gggataagca cgaaggcctc ccaaaaccag gtggtatgac
     3241 cgagatgcca tttggggggg ctcacatcca cgacgggctg cacgacatag tcctcctttt
     3301 gcagggggcg gcagatatct tcgctcgcct tgcggacagc gcggtattga tcgaacaagg
     3361 tcattattga tccgttttca aataatttgt aagatcggag atcgtccgga tgcccagctg
     3421 ctgcatctgg ctgcggcgca tcatcagggc ataggcattg ttgaagccga tgggttccag
     3481 ccaggtgagc cggtactcct tgtcgaaacg atcggcgaca taccggtata cgctgtcccg
     3541 gctgccgcta agccgggcag tcaggtcgga aggcggctgc aggatcg
//
LOCUS       DATGFG010000709         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1155029, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000709 DATGFG010000000
VERSION     DATGFG010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155029"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VKQ52_13255"
     CDS             complement(<1..774)
                     /locus_tag="VKQ52_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88213.1"
                     /translation="MLTRFFLSVSLVLVCCGVLFSQRPCKRSSPLFRFDPHARPRMFI
                     EKLGNHPQFPFLQYEKGIVTRALFLKAVKDADSRRQYNIEFDVFNRLLQDIGFVNGYK
                     DLRAADIENLFLNPGITGNLGFFNRESNYIYVKLNPAGEGDDGIAAWKITGPGGCYFY
                     ILHTCGNAFVDEADSGRPCPCRGWTTPGKHRGGDTVVRIVHSIDTVTRADTTYIRLPG
                     DGKEDCRKKWEITLDGGVSFNSAPRFDNTTQHSRPDGARP"
     gene            939..1943
                     /locus_tag="VKQ52_13260"
     CDS             939..1943
                     /locus_tag="VKQ52_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88214.1"
                     /translation="MKTNNIQHLLFATSLSMVLFACNKSNSSSSSSTPPSNAQLQTQS
                     DDESQVSNESNAAFDDVNTAMTSQATITGSSENSPVSYGNGVMVEGGNQDTVKSYICD
                     ATVTVDTVDNPHTITISYNGSNCNVTRIRKGSIVISWVPGMKWTKAGAVVTVNFDSLK
                     ITRVRDGKTIIFNGTHTYTNVSGGSLLSLTSNSATPIIHTITSSNMSITFASDSVRSW
                     SVARQRQYSYNNGLVITTTGLQTQGTVSGISEWGTTRFGNSFTVQIAQPLILSQSCGF
                     QLTSGEHILTDAKGTTDITYGLDATGAATSCPISGGTYYFKLAWTGSAGKTYTFILPY
                     "
     gene            2012..2911
                     /locus_tag="VKQ52_13265"
     CDS             2012..2911
                     /locus_tag="VKQ52_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88215.1"
                     /translation="MKSLALVILFAGALLACSKKTSPFNNTAAFASELQTQANDEIRI
                     STELGAAFNDVDSVIANATSVCGGAVTVDSVDTPRRITVVYSGNTCDALRSRTGSITI
                     SYTPGTSWTTAGDSVTVTLTSLVITRLADSKTLTFNGAFIYKNISGGSLSGLSSGGTS
                     PVVHTLAGYNVTVTYDNTTATNWQFSRQRSYTYNSGLVISTLGIDSAGGIGSVADWGA
                     NRFGNSIITAFTSPLLVNQGCSWRVTGGQATMTNPIGISTLTFGLDSTGKTTGCPVSG
                     GHYYYQLSWTGDTESPYSAVLSY"
     gene            2949..>3737
                     /locus_tag="VKQ52_13270"
     CDS             2949..>3737
                     /locus_tag="VKQ52_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLZ88216.1"
                     /translation="METITQTVTTDWLQSIEALAEVPAPQLQWLIDNGQLRPITEGEF
                     IFRPGDAITGMYIIMSGKIKIYMLQKQEIMEVNVLEPKTISGALPFSRAVKANFNAKV
                     LQSGELLILPVEKFREMVSKHYELTGALVHVMTTRVRNITAMQQQNEKMMALGKLSAG
                     LAHELNNPAAAIVRGSESLVQHLQLEPELFKDVINVRIEAKDVDFIKDKLFEILARKE
                     KPKLTLVQRTELEDELRDWLEDNKVDSAAEVAENFLEYSFTCEDM"
BASE COUNT          884 a         1021 c         1021 g          811 t
ORIGIN      
        1 gggcctggcg ccatccggcc ggctgtgctg ggtggtattg tcaaagcggg gagcggaatt
       61 aaaggagacg ccgccatcca gcgttatttc ccatttcttc cggcaatctt cctttccgtc
      121 gccgggaagc cggatatagg tggtatcggc ccgggtgacg gtgtcgatgc tgtggacgat
      181 ccggacgacg gtatccccgc cgcgatgttt tccgggggtg gtccatccgc ggcagggaca
      241 gggccttccc gaatcggctt cgtcgacaaa ggcattgccg caggtgtgca ggatataaaa
      301 atagcagcct ccggggccgg tgatcttcca ggcggcgatg ccgtcatcgc cctctccggc
      361 ggggttcagt tttacataga tgtaattgct ttccctgttg aagaatccca ggttgccggt
      421 gatgcccggg ttgagaaaga ggttttcgat atcggcagcc ctgaggtctt tgtatccatt
      481 gacaaacccg atatcctgca ggagccggtt gaagacatcg aattcgatgt tgtattgtct
      541 gcggctgtcg gcatctttca cggccttcag gaacagggcg cgggtgacga tgcctttttc
      601 atattggaga aacgggaatt gtggatggtt gccgagtttt tcgatgaaca tgcggggacg
      661 ggcatgcgga tcgaaccgga acagggggga agatcttttg caaggccgtt gcgaaaataa
      721 tacgccgcaa caaaccagga caagggatac ggacaggaag aaacgcgtta gcattcaacg
      781 ggtttaaggg gtagcgacca gggaaaaacg aaaaaataac atctttctta tatcgtttaa
      841 aaaataatat attttttccc ccgttaaact aagtattcta ccattcgtcc taaataaaac
      901 gtttacccta aaaactaaaa acctactaaa tcgaaattat gaaaaccaac aacatccaac
      961 atcttttgtt tgccacttcg ctctccatgg tattatttgc ctgtaacaag agcaacagct
     1021 cttcaagctc ttctactcct ccttcgaatg cccagcttca aacgcaatca gatgacgaaa
     1081 gccaggtcag taacgaatcg aacgccgcat tcgacgacgt gaataccgcg atgaccagcc
     1141 aggccaccat caccggcagc agtgaaaatt ctccggtgag ctatgggaat ggggtcatgg
     1201 tggagggagg aaaccaggat accgtgaagt cctatatttg tgatgccacc gttacggtgg
     1261 ataccgtcga caatccccac acgatcacta tttcgtacaa tggttccaac tgtaatgtga
     1321 cgcgtatccg taaaggttct atcgtcatct cctgggtccc gggtatgaaa tggaccaagg
     1381 ccggcgctgt cgtgacggtc aatttcgaca gcctcaagat cacccgtgtc agggatggca
     1441 agaccattat cttcaatggc acccatacct acacgaatgt cagcggcggc agcctgctga
     1501 gccttacttc caattcggct accccgatca tccacacgat caccagttcc aatatgtcga
     1561 tcaccttcgc cagcgattcg gtacgttcct ggagtgtggc gcgtcagcgg cagtattcct
     1621 acaataacgg cctcgtgatc accacgacgg gtctgcaaac ccagggcacc gtatccggta
     1681 tttccgaatg gggtacgacc cggtttggca acagttttac ggtacagatc gcccagcccc
     1741 tgatcctgtc acaaagctgc ggctttcagc tgacttccgg tgagcacatc ctgacggatg
     1801 ccaagggcac gacggatatt acgtatggcc tcgatgccac cggcgcggct accagctgcc
     1861 cgatcagcgg cggaacatac tatttcaaac tggcatggac gggttcggcc ggcaagacct
     1921 acaccttcat cctgccttat taacgttact ttcatattta tgacagacgg ccccaaaagg
     1981 gccgtctgtt tttttgtgta cattcgtgcc catgaaatct cttgcattgg tgatcctttt
     2041 tgcgggggcg ttgctcgcct gttcaaaaaa aaccagtcca ttcaataata cggcggcctt
     2101 tgcttccgaa ttgcagaccc aggccaatga cgagatacgc atctccaccg agctgggagc
     2161 cgcgttcaat gatgtggaca gcgtcatcgc caatgcgacc tccgtctgcg gcggggcggt
     2221 aaccgtcgac tccgtcgata cgccgcgcag gatcaccgtc gtttacagcg ggaatacctg
     2281 tgacgccctg cgcagccgca cgggttcgat cacgatctcc tatacacccg gcaccagttg
     2341 gaccaccgcg ggcgattccg tcaccgtcac gctcaccagt ttggtgatca cccggctggc
     2401 ggatagcaag acccttactt tcaatggggc attcatctat aaaaatatca gcggcggcag
     2461 cctgtccggc ctgtcttccg gcggaacgag cccggtggtg cataccctcg ccggatacaa
     2521 tgtgactgtc acgtatgata ataccaccgc aaccaactgg cagttctccc ggcaacgcag
     2581 ctacacctat aacagcgggc tggtcatctc gacgctgggg atcgactccg ccggcggcat
     2641 cgggagcgta gccgactggg gcgccaaccg ctttggcaac agcatcataa cggcctttac
     2701 ttctcccctg ctggtcaacc agggctgcag ctggcgggtt acgggcgggc aggcgacgat
     2761 gaccaatccg atcggcatct cgaccctgac cttcgggctg gattccacgg ggaagacgac
     2821 gggctgtccc gtcagcggcg gtcattatta ttaccagctt tcgtggacag gcgatacaga
     2881 atccccctat agcgccgtgc tctcctactg accgttgaaa agtagcaaat attgattaca
     2941 ttgccggtat ggaaaccatc acacaaaccg tgacgacgga ctggttgcag tccatcgaag
     3001 cgctggccga agtaccggcc ccccagttgc agtggttgat cgacaacggc cagctccggc
     3061 cgataacgga aggagaattt atcttccggc cgggagacgc cattaccgga atgtatatca
     3121 tcatgtcggg taagatcaag atctacatgc tgcagaaaca ggagataatg gaagtgaatg
     3181 tcctggagcc aaagacgatc agcggggcgc tccccttttc gcgggcggtg aaggccaatt
     3241 tcaacgccaa ggtcttgcag agcggggagc tgttgatcct gcccgtggag aagttccggg
     3301 agatggtcag caaacattac gagctgacgg gggccctggt ccatgtgatg accacccggg
     3361 tacggaacat tacggccatg cagcagcaga acgagaagat gatggccctt ggcaaattgt
     3421 cggccgggct ggcccatgag ctgaataatc ccgcggccgc catcgtaagg ggatcggaat
     3481 cactggtaca acatttgcag ctcgaaccag agctcttcaa ggatgttatc aatgtccgga
     3541 tcgaggcgaa ggatgtcgac ttcataaagg acaagctctt cgagatcctg gcgcggaagg
     3601 aaaaacccaa actgaccctc gtgcagcgga cggaactgga ggacgagttg cgcgactggc
     3661 ttgaggacaa caaggtcgac agcgccgcgg aggtggccga gaacttcctt gaatatagct
     3721 tcacctgcga ggatatg
//
LOCUS       DATGFG010000710         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1608050, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000710 DATGFG010000000
VERSION     DATGFG010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608050"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VKQ52_13275"
     CDS             <1..252
                     /locus_tag="VKQ52_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88217.1"
                     /translation="QPSEAWAWWKRTGFPNTTSTIAWEPLTTAGTAISLPRRAAITVL
                     LPTDRNYDNQQAAFKNMASDGNWGTGPNDFSGRVWWDMP"
     gene            330..836
                     /locus_tag="VKQ52_13280"
     CDS             330..836
                     /locus_tag="VKQ52_13280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLZ88218.1"
                     /translation="MKKLLLSMFVSCAGVFSGAQPAPTPLRVVIFLSPECPLCQNYSL
                     PLNQLFKKYAGKVQFTGVVPGKAYSTADVNAFTQKYHIGFPVYIDSARTMSRSLTATV
                     TPEVFLLGPDKKVLYYGSIDNWIKDLGMQSARPTVFYLQDAIDQSLAHQPIRIPYNKP
                     VGCLINDY"
     gene            838..2256
                     /locus_tag="VKQ52_13285"
     CDS             838..2256
                     /locus_tag="VKQ52_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88219.1"
                     /translation="MKRILLNIALLLTLAGPIPSLAQSSRPTGSLAQSSSSAGSLAQS
                     SAAPTHAAVQSSPTFTDVAPIIRTKCATCHKPGQTGPFSLITYADVAKRTSFIRDVLQ
                     KGYMPPWKADNQYVHFANDRSLTDKEKTLLLQWIDNKAPRGDGPDTARLKEASIPGTS
                     YFRKPDLVLKAKDSFRVTGDNIERYIIFKIPFELKDSANVEAIELYCNNKKLIHHANY
                     AIHSVPDTTIDIDHTDALLNLTEDDRRKFDQYQPYRKTITYYGGWIPGTSYESYPKDI
                     GWVMPRRGVILLTVHFAPSAVDESVMLGVNLFFKKTPVQRRVKVVSFGSGGIGEKQID
                     PLFFIKAGQVQKFTLEVTNPTDDQSILYVWPHMHLIGKEYLAYAIPPNSSDTIRLVHI
                     PRWDFRWQEIYRFQKLVKIPKGSVIHLECTYDNTADNPFNPNSPPRNIFSYGDMRTTD
                     EMMTLMMIFLPYRSGDENIELQ"
     gene            2268..>3171
                     /locus_tag="VKQ52_13290"
     CDS             2268..>3171
                     /locus_tag="VKQ52_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ88220.1"
                     /translation="MKQNTPIGNPGLFVRFIAFIAATLAAAGAYAQAKTPLFTRLDAK
                     QTGISFVNTIHEDDSLHVMRYEYLYNGAGVGIADLNNDGLDDIFFSGNTGPCKLFLNK
                     GNFRFEDVTRQAHVAGNGTWCTGVSMADVNGDGLMDIYVCHSGKYDDTTKLSNELYIC
                     QGIKDGIPVYKEMAKEYGLDAPGTQSTQAVFFDYDKDGDLDMFLLNHSNHTYNPYLNT
                     RKTRATPDMHFGNRLFRQDRDAAGHIHFTDVTLQAGIINNPLNFGLSVTVSDVNGDGW
                     PDIYTSSDYTERDCFYLNNHDGTFT"
BASE COUNT          779 a         1035 c          731 g          626 t
ORIGIN      
        1 caacccagcg aggcctgggc ctggtggaaa cggacgggct tcccgaacac gacctctacc
       61 atcgcatggg aaccactcac caccgccggc acagctattt ctttgccccg gcgggcggcg
      121 atcaccgttc tgctcccgac agaccgcaac tacgacaacc agcaggcggc cttcaagaac
      181 atggcctccg acggcaactg gggcaccgga cccaacgact tcagtggtcg cgtctggtgg
      241 gacatgcctt agccaacagc aaaatacatc ggactccggg ctgttatcag cccggagttt
      301 tttaatttta agaactgaaa ttgggtggca tgaagaaatt gcttttgtcg atgtttgtat
      361 cgtgcgcagg ggtattctca ggggcccaac cggcccccac ccccttacgg gtcgttatct
      421 ttctttcacc cgaatgcccc ctttgccaaa actattccct gccgctgaac cagcttttca
      481 aaaaatacgc cggcaaggtt caattcacgg gcgtcgtgcc gggaaaagct tattccaccg
      541 ccgacgtcaa cgcattcacc caaaaatacc acatcggctt tcccgtatat atcgattcgg
      601 ccaggactat gtccaggtct ttaacagcga ccgtcacgcc cgaagtgttt ctgctcggcc
      661 cggataagaa agtactatat tacggctcca ttgacaactg gataaaggac ctgggtatgc
      721 agagcgccag gccaaccgtt ttttacctgc aggacgccat cgatcagtcg ctggcgcacc
      781 aacccatccg cattccctat aacaaacccg taggctgcct gatcaacgat tactgatatg
      841 aaacgaatcc tgttgaatat cgccctcctc ctgacccttg ccggacccat accgtccctc
      901 gcccagtctt cgcgccccac cgggtccctc gcccagtctt caagctccgc cgggtccctc
      961 gcccaatcct ccgcagcccc gacgcacgcc gctgtacaat catcgccgac ttttaccgac
     1021 gtagccccca tcatccgcac caaatgcgcc acctgccaca aacccgggca gaccggtccc
     1081 ttttccctta tcacctatgc cgacgtggcc aaaagaacat cctttatccg ggatgtcttg
     1141 caaaaggggt atatgccgcc ctggaaggcg gacaaccagt acgtccactt tgccaacgac
     1201 cggtcgctca ccgataaaga aaaaacactg ctgctgcaat ggatcgacaa caaagccccc
     1261 cgcggcgatg gccccgacac ggcccggctg aaggaagcct ccatccccgg caccagctat
     1321 ttccgcaagc ccgacctggt cctgaaagcg aaggacagtt ttcgcgtgac gggtgacaac
     1381 atcgaacgct atatcatctt caagataccc ttcgagctaa aagactcggc caacgtcgaa
     1441 gccatcgagc tctactgcaa caacaaaaag ctgatccacc acgcgaacta cgccatccac
     1501 tccgtacccg acacgactat cgatattgac cacaccgacg cgctgctcaa cctcaccgag
     1561 gacgaccgcc ggaaattcga ccaataccaa ccctaccgca agaccatcac ctactatggc
     1621 ggctggattc ccggcaccag ctatgaatcc tatcccaaag acatcggctg ggtgatgccc
     1681 cgccgcgggg tgatcctcct caccgtccac ttcgctcctt cggcagtaga cgagtccgtc
     1741 atgctggggg tcaacctctt cttcaaaaag acgccggtac agcgcagggt gaaagtggtc
     1801 agcttcggat cgggcggcat cggcgaaaaa cagatcgatc ccctcttttt tataaaggcg
     1861 ggacaggtgc agaaatttac gctcgaggtg accaatccca ccgatgacca gtcgatcctg
     1921 tacgtatggc cgcatatgca tcttatcggg aaggaatacc tggcatatgc cattcccccc
     1981 aacagcagcg ataccatcag gcttgtacat atcccccgct gggacttccg ctggcaggag
     2041 atctaccgtt tccaaaagct cgtgaagata cccaaaggct cggtgataca cctggaatgc
     2101 acctatgaca atacggccga caatcctttt aatcccaaca gcccgccgcg aaacatcttt
     2161 tcctatggag atatgcggac caccgatgaa atgatgacgc tgatgatgat attcctgccc
     2221 taccggtccg gggacgagaa tatcgaactg caataaaatc catcgcattg aagcagaaca
     2281 caccaatcgg caaccctgga ctatttgtcc ggtttatcgc tttcatcgcc gcgacactgg
     2341 cggccgccgg cgcctatgcg caagcgaaaa cacccttatt cacccgcctc gatgcgaaac
     2401 aaaccggtat ctcttttgta aacaccatcc acgaagacga ctcgctccat gtcatgcgct
     2461 atgaatacct ctacaacggc gcaggggtag gcatcgccga tctcaacaac gacggcctcg
     2521 acgatatctt tttttccggc aacaccggtc cctgcaaact cttcctcaac aaggggaatt
     2581 ttcggttcga ggacgtcacc cgccaggccc atgtcgccgg caacggaacc tggtgcaccg
     2641 gcgtaagcat ggccgatgtc aacggcgatg gactgatgga tatctatgta tgccactcgg
     2701 ggaaatacga cgacacgaca aagctctcca atgaactata catctgccag ggaataaagg
     2761 acggcatccc cgtctataag gaaatggcaa aggaatacgg gctcgatgcg ccgggaaccc
     2821 agtctacgca ggccgtcttt ttcgactacg acaaggacgg cgatctggac atgttcctcc
     2881 tcaaccattc caaccacacc tacaacccct atctcaatac ccgcaagacc cgcgccacgc
     2941 ccgatatgca cttcggtaac cggctctttc gccaggacag ggacgccgcc ggtcacatcc
     3001 atttcaccga cgtgaccctc caggcgggca tcatcaacaa cccgctgaac tttggactca
     3061 gtgtcaccgt cagcgatgtt aacggggacg gctggccgga tatttatacc agcagcgact
     3121 ataccgaacg ggactgcttc tatctcaaca accacgacgg caccttcacc g
//
LOCUS       DATGFG010000711         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_607784, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000711 DATGFG010000000
VERSION     DATGFG010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607784"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VKQ52_13295"
     CDS             complement(<1..1052)
                     /locus_tag="VKQ52_13295"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ88221.1"
                     /translation="MRKSLLLICMIVSSYLLHAQKIVTGKVLDAKSGTPLAGASVKSR
                     LSKAGTSTGSDGSFRIQVAETNDVLEISIIGYSPQSFPLDGRSEMTIALQPASSELSE
                     IVFVGSRGAGRAKTETPVPVDVIRVNQVGMPTGKMELTSLLNMTAPSFNYNKQSGGDG
                     ADAIDLATLRGLGPDQTLVLINGKRQHQTAFVALFGTRGRGNSGTDLNAIPEASIDRV
                     EILRDGASAQYGSDAIAGVMNIILKRDVNHLAVSAGYSAYYDHKYNSLNSVQPSQYVT
                     GSRFDGQTFTFGLNHGFSIGKNNGFLNYSLNFMSQGKTFRQVPDTNIFSNPKALPVNN
                     VRRAMGDASVTSGGIM"
     gene            1253..1708
                     /locus_tag="VKQ52_13300"
     CDS             1253..1708
                     /locus_tag="VKQ52_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLZ88222.1"
                     /translation="MNEQQKQAIASLGRRFSDATIFLHEAIAHRGGLTVTDHKYLGLI
                     MQKGPMTAGELSQLAGLTTGAITGVVDRLEKKKLVKRQFDKSDRRKIIIVPNLENATK
                     LFGGVQTQLRTRIFNMIATFSDDELNVIERYLQMTIDVMDEITVDIKNK"
     gene            2248..2661
                     /locus_tag="VKQ52_13305"
     CDS             2248..2661
                     /locus_tag="VKQ52_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLZ88223.1"
                     /translation="MRTTQTLYWIFTGLFAFMMLGSAIPDVLSKEIAVKGMHDNLGYP
                     LYFIPFIGMAKVLGVIAILVPGFPKIKEWAYAGLFFDLAGATYSIVASGGSAGQWGFM
                     LLPIAFGGLSYLYYYKRQASQTKAKTLVAGTASRA"
     gene            complement(2683..>3051)
                     /locus_tag="VKQ52_13310"
     CDS             complement(2683..>3051)
                     /locus_tag="VKQ52_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88224.1"
                     /translation="DQPDQAVQVLILPEELRGLNEGSFLLPADKDAESAGRMTNGSAS
                     DVGNSGASRMSNGSVSEADTTSLTLQYQGHARTVNVPPNTPVVEYKVSGKKPNPGDKI
                     FLLVKKGDHDTLTSAKIVCF"
BASE COUNT          711 a          713 c          858 g          769 t
ORIGIN      
        1 atcatgatcc cgcccgaagt gacggaggca tcgcccatgg ccctgcggac attgtttacg
       61 ggaagggcct tcggattgct gaagatattg gtgtccggca cctggcggaa ggtcttgccc
      121 tggctcatga agttgagcga ataattgagg aagccgttgt tcttgcctat ggaaaatcca
      181 tggttgaggc caaaggtgaa ggtttgcccg tcgaaccggc ttcccgtgac atactggctg
      241 ggctggacgc tgttgaggga attgtatttg tggtcataat aagcggaata gccggcgctt
      301 accgcgaggt ggttgacatc ccgcttgagg atgatgttca tcacgccggc gatggcgtcg
      361 gagccatatt gtgccgaggc gccgtcgcgg agtatctcca cgcgatcgat ggaggcttcg
      421 gggatcgcat tcaggtcggt tccggagttg ccgcggcctc gggtcccgaa gagggccacg
      481 aaggccgttt gatgctggcg tttgccattg atgaggacga gggtctggtc gggtcccagg
      541 ccccggaggg tggcgaggtc gatggcatcg gcgccgtcac caccgctctg cttattataa
      601 ttgaaggagg gagccgtcat attgagcagg gaggtgagct ccattttgcc ggttggcatg
      661 cccacctggt tcaccctgat gacgtccacc gggacggggg tttccgtctt ggcgcgcccg
      721 gcgccgcggc tgccgacgaa gacgatctcc gagagttcgc tggaggcggg ttggagggcg
      781 atggtcattt ccgatcgacc gtcgaggggg aaggattggg ggctgtaccc gatgatgctt
      841 atttccagta catcatttgt ctctgccacc tggatcctga agctgccgtc gctgccggtg
      901 gaggttcctg ctttggaaag gcggcttttt accgaggcgc cggcgagtgg cgtccctgat
      961 ttggcgtcga ggactttacc ggtgacgatt ttctgtgcat ggaggaggta ggagcttacg
     1021 atcatgcata ttagtagcag tgactttctc atgaggcaag ttttcgtttt cgttacttca
     1081 agttacccct attattcgga ttggggaaaa aaaaggcgac ggacacgggg gtatgttgga
     1141 gaaggggggc gtcaaaaaaa gaccggaaac ttttcgtggt gtgatcattt ctatttatct
     1201 tagctaataa atatcttagc aactaagaca tatggaccgg aaaaacagag ttttgaatga
     1261 acaacagaaa caagcaatag ccagcctggg caggcggttt tccgatgcga ccatattcct
     1321 tcatgaagcc attgctcaca gaggggggct gacggtcacc gatcataaat atctagggct
     1381 tatcatgcaa aaaggcccca tgaccgcagg cgaactttcc caactcgccg ggttgacaac
     1441 gggagctatt accggtgtgg tagacaggct tgaaaagaaa aaactggtca agcggcaatt
     1501 tgataaaagt gaccggcgga aaataatcat tgtcccgaac ctggagaacg ccacaaagct
     1561 tttcggaggg gtacaaaccc aactgcggac aagaatattc aatatgatcg caacgttttc
     1621 ggacgatgag ctcaatgtga ttgaaagata cctgcagatg acaattgacg taatggatga
     1681 aataacggtc gatataaaaa acaaataaga tggagcaact aacaggggaa tatctgtcca
     1741 gggccgggac ctggcttttt ataacaaccc ttgcctactt tttactgaat ggcgcccagg
     1801 tttttgagac ggccgtgatc gtgccgaagt ggacggcatc accgcctgaa tccttccaac
     1861 tattcaaagg aaaatatggg ctcgacctta aacggttttg gataatcatg catagccttc
     1921 atgaaataac gtttattctg gccatcgttt tctgttggaa aatagacccg ataaggaact
     1981 ggcttttgct cttattcgcc ctgcatttcg ccgtaagggt ttggacgatt gtatattttg
     2041 cgccagacat tattgaattc gggaagattg ccaacacttc ctacgccggg accggcttgt
     2101 caggcaggac ggttcgttgg cgggcattga actatgtaag agttgggatc tacattattc
     2161 tttccatcgg atttattccg ctgtgcataa aacttttgga cctaaaatat aaataaggac
     2221 cgaattccaa ctagtaaaac aactaaaatg agaacgacac agacactcta ttggatcttt
     2281 accggattgt tcgccttcat gatgcttgga tccgccattc ccgatgtgct cagcaaggaa
     2341 attgcagtta aaggtatgca cgataacctg ggctatccgt tgtattttat tccttttata
     2401 ggcatggcta aagtgctggg ggtgattgcc atcctggtac ccggctttcc gaagataaag
     2461 gaatgggcgt acgcaggctt gtttttcgac ctggccggcg caacgtattc gatagtcgcc
     2521 agtggcggga gcgcagggca gtggggcttt atgttactgc ctattgcgtt tggcggattg
     2581 tcgtatctgt attattataa aagacaagcg agccagacaa aagcgaaaac cctggtcgcg
     2641 ggaaccgctt ccagggcttg atctttttac cgttgaacgt gatcaaaagc acacgatctt
     2701 agcgcttgtc aacgtatcgt gatcgccttt tttcaccagc aggaagatct tgtcccccgg
     2761 gttaggtttt ttacccgata ccttatattc cactaccggc gtattcgggg gcacatttac
     2821 cgttcttgca tgtccctggt attggagcgt cagtgatgtg gtgtcggctt ccgatacgct
     2881 tccgttcgac atccgggaag caccgctgtt gcccacgtcg gaagcggacc cattggtcat
     2941 gcgaccagca ctctcagcat ctttgtcggc gggcagtagg aagcttccct cgttcaatcc
     3001 ccgcaattct tccggcaaga tcaacacctg gacggcctga tccggctggt c
//
LOCUS       DATGFG010000712         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1326016, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000712 DATGFG010000000
VERSION     DATGFG010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326016"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VKQ52_13315"
     CDS             <1..684
                     /locus_tag="VKQ52_13315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ88225.1"
                     /translation="LTAAIGEVSRAYQNQKQFTENAAHELQTPLAVIRSKVELLMEDP
                     VLTEDTAQLLADINEANERLSQMNKNLLLLTRIDNRQYPEQRTVLLSEMLTRMTAYYE
                     EQGEPDIPRIKTQIQPGIQVVANPSLIEILLNNLLRNAVIHNIPGGYIDVSLTGGELV
                     VENSGPLLLEDPERLFERFRKGNEAARTTGLGLALVRQICQLYRYEINYQHSRGTHRL
                     RLSFASPAF"
     gene            745..2133
                     /locus_tag="VKQ52_13320"
     CDS             745..2133
                     /locus_tag="VKQ52_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ88226.1"
                     /translation="MLPLRFYLPAVCAFVASFSLNPAALAQRRTLSVREVLSLIQTEQ
                     PLLRAYKARSAAARYDIDLARNTLVPNLTGGYQAGYATDNNITGMSYPGLLMPISGPV
                     AAHSSNSFIPGTALAAYLQWTPLTFGQRDAAVEKAAAQFSLSGTAYDNALFQQQYAGI
                     LTYFNIVYLQKLLGTQQANIDRTRVGLEQSLVLATQGLRPGLDTVQFQAVLSQAIMER
                     LNTQRLYQAQVLELCRLTGLAASPDDLSFADTAMTLGLPAQPDTSGGYAQNPGYRYYQ
                     AQVAVSAATLKEAERGWRPRLDIWANTYSRGSGIDPGGVINKADGWALSRTNVGAGVQ
                     LSFPILQFSRVAIQKKQYRLLLQADRDQLAQADLNLRRQVETAEYNYRQNLLVAHEAP
                     VESRAARLSYQGLQLSYRSGLVDFTRLTQGQYQLLNAEMSEADANLQVWKALLDIGVA
                     KGNLHLFTDQLK"
BASE COUNT          460 a          680 c          606 g          441 t
ORIGIN      
        1 ctcaccgcag cgatcgggga ggtgagccgc gcctatcaaa accagaaaca attcaccgag
       61 aatgcagctc atgagcttca gaccccgctg gcggtgatcc ggagcaaggt agaactgctg
      121 atggaggacc ccgtcctgac agaggacacc gcgcagttgc tggccgatat caacgaagcc
      181 aatgaacggc taagccagat gaacaagaat ctcctcctgc ttacccggat cgataaccgg
      241 caataccccg agcagcggac ggtgctcctt tccgagatgc tcacgcggat gaccgcgtac
      301 tatgaagaac agggtgaacc ggatattccc cggatcaaaa cccaaatcca gcccggcatc
      361 caggtggtgg ccaatccttc cctgatcgag atcctgttga ataacctgct gcgcaatgcc
      421 gtgatccata acatccccgg tggttatatc gatgtcagcc tgacgggggg tgagctggtc
      481 gtggagaata gcggtccgct gctgctggaa gaccccgaac ggctgttcga gcggttccgg
      541 aaggggaatg aagcggcccg gaccacgggc ctcggtcttg cgcttgtgcg ccagatatgc
      601 cagctctacc ggtatgagat aaactaccag cactcgcggg gtacgcatcg tctgcggctt
      661 tcttttgcct cgcccgcttt ctaaattctt cctaaatcgt cttttagctt tgttatttat
      721 ccatatatag aaagctgatt atatatgctt cctctccgat tctatctgcc ggcagtctgt
      781 gccttcgtgg caagcttttc cctcaacccg gcggctcttg cccaacggcg aaccctttcc
      841 gtcagggagg tcctttccct tatccagacg gaacagccgc tgctcagggc ctataaagcg
      901 cgatccgccg cggcccgcta tgatatcgac ctggcgcgca ataccctggt gcccaatctc
      961 accggcggct accaggcagg gtatgccacg gacaataata tcacgggtat gagctatccc
     1021 gggctgttga tgccgatatc gggacccgtg gccgcccaca gcagtaacag ctttattccc
     1081 ggcaccgccc tggcggctta cctgcaatgg acgcctctta cgttcgggca gcgcgatgcc
     1141 gccgtcgaaa aggcggccgc ccagttcagt ctttcgggga cggcctatga caatgcgctg
     1201 tttcagcagc aatacgcggg catcctcacc tacttcaata ttgtctacct gcaaaaacta
     1261 ctgggtaccc aacaggccaa tatcgaccgg accagggtag gtctggagca atcccttgta
     1321 ctggccacgc agggactacg ccccggactg gatacggtgc agtttcaggc cgttctctcc
     1381 caggccatca tggaacggtt aaacacccag cggctttacc aggcgcaggt actggagctt
     1441 tgccggctga ccgggctggc cgcgagtccc gatgatctga gcttcgccga taccgccatg
     1501 accctgggcc tgcctgcgca gcccgatacc agcgggggat atgcgcaaaa tcccggctat
     1561 cgctattacc aggcacaggt ggcggtgagc gcggctactc tcaaggaggc cgagcgcggc
     1621 tggcgacccc gcctcgacat ctgggccaac acctattcgc gtggatcggg catcgatccc
     1681 ggcggcgtga tcaataaggc ggacggctgg gccctctccc ggacgaatgt cggcgccggc
     1741 gttcagctta gctttcctat cctgcaattc tcccgcgtgg ccatccagaa gaagcaatat
     1801 cgcctgctgc tccaggccga ccgggaccag ctcgcccagg cggacctcaa cctccggcgg
     1861 caggtagaga ccgccgaata caactatcgc cagaacctgc ttgtcgccca tgaggcgccc
     1921 gttgagtcca gggctgcgcg gctctcctat caggggctgc agttgagcta tcggagcgga
     1981 ctcgtggatt tcacccggct cacgcagggc cagtaccaat tgttgaatgc cgagatgagt
     2041 gaggccgatg cgaatctgca ggtctggaag gcgctgctcg atatcggcgt cgccaaagga
     2101 aacctccatc tattcaccga ccaactgaaa taatcccgga actaccatga agcttatcgt
     2161 ttcagcattg cggcatccta tcacgat
//
LOCUS       DATGFG010000713         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_26574, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000713 DATGFG010000000
VERSION     DATGFG010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26574"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VKQ52_13325"
     CDS             complement(<1..861)
                     /locus_tag="VKQ52_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88227.1"
                     /translation="MPNNPTNLFTNAHSHIFTVSDAPDYFLKTAIKSKWLAELVDKML
                     QKKGTRAVLQAIHWIIKPFSPNYRNTVERYIEFVEIGTSATQQEILDKESQAYSKVGT
                     YRIVALTQVLDYLDYNQTSNHKKIQTQVEEVANLKRNALYQDRLYPFLGIDPRMQGTD
                     LLQWVKKYVSKDYGFYGIKIYPAYGFFPFDNRLAPVWEWAAGEGIPVMSHSTRGGSFY
                     LGNFSSILNSGGFVPQSLNPNSATMPAIKDRINSLVKDGSVQKNNKVWCNIFGHIDNY
                     RVVLEDNRFKN"
     gene            complement(891..2084)
                     /locus_tag="VKQ52_13330"
     CDS             complement(891..2084)
                     /locus_tag="VKQ52_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88228.1"
                     /translation="MLSSLTNSPLFETIICLILAFALLSLLVSTLTEVVNSYFNERGQ
                     QLYIVISRMFDDGLNVNFGQLLYSHPMITNIRKDTNSLPQYISNNMFSQVLIDVIGNY
                     GRSYKFDAAANAIALQNDPGKGAITDPFLLFQAGVNNMKHTDLKLMLMNMVEKSASLS
                     GKELEQRLAQLDGMIQQWYNDQMDRTSGWFKDLMRKRLLIISLLVAVGLNVDSIHLFK
                     NLYTHPTLRSQLLPVAEVVADNLAKAKTDTTLTALQQVYNAVAASSLKHPGKDSSYAA
                     LHGILRDLKEIDSLQQHRDSTRIANLQTASDILDDLAGLNIPIGWHNGIPPLNLRPRT
                     PEAKRHWEQASSWDRFGTIAALLFWYVLGIAITAFSISAGAPFWFDLLLKLVNIRRAG
                     KKPES"
     gene            2324..2908
                     /locus_tag="VKQ52_13335"
     CDS             2324..2908
                     /locus_tag="VKQ52_13335"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HLZ88229.1"
                     /translation="MQIIQSIQNRIHEVRGERVLLDKDLAALYETETKALNLAVKRNL
                     KRFPKDFMFQLTKEEWDTLRLQTETSNHFQAPANSTEPLRLQIETSKSGRGGTRYLPY
                     AFTEQGVAMLSGILSSDKAINMNIAIMRAFVEVRRIIYQQSDLKLQLQELKDHIGAHD
                     VQLNQIYDAMENLLDEKASQRKWDDRDRIGFRQR"
     gene            3156..3725
                     /locus_tag="VKQ52_13340"
     CDS             3156..3725
                     /locus_tag="VKQ52_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88230.1"
                     /translation="MDKAFVSILTGISPLVFAYVFKRMESRSALAINKGKLEEASNRI
                     VFAEKYLQLQAKVLSDNELSVLNKEVSADLFRLKQDIDTLFIKTEKAHNLKVFSVQKL
                     FLTFRPASWLGWVLAFLFYLSLMFLAFYTMGLFIDDNYNFSFDAFKKQLQDGDTIAGF
                     VICLSLALLIRYLAVSAYKRKTAHALAHP"
     gene            3712..>3914
                     /locus_tag="VKQ52_13345"
     CDS             3712..>3914
                     /locus_tag="VKQ52_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88231.1"
                     /translation="MPIHSKANLPTPRWHDFFILHPVMRHPILSKGKLLLFTCIILIA
                     SRAMSQDGKALFQTNCAQCHNPI"
BASE COUNT          884 a         1066 c         1026 g          938 t
ORIGIN      
        1 gtttttgaat ctgttatcct ccagcacgac ccggtagttg tcgatatgtc caaatatatt
       61 gcaccaaacc ttattgttct tttgcacgga gccatctttt accaacgagt ttatccggtc
      121 ttttatcgcc ggcatcgtgg cactgttggg gtttaggctt tgcggaacaa aaccgccgct
      181 gttgaggatc gacgaaaagt tccccaggta gaaggatccg ccgcgggtgc tatggctcat
      241 gacgggtatg ccttcgccgg cggcccattc ccatacgggg gccaggcggt tgtcaaaggg
      301 aaagaacccg taggcggggt agatcttgat gccgtagaag ccatagtcct tactgacata
      361 tttctttacc cattgcagca ggtctgttcc ctgcatccgc gggtcgatac ccaggaaggg
      421 atacagccgg tcctggtaca gggcgttcct tttcaggttc gccacttctt ccacctgggt
      481 ctggatcttt ttatggttgg aagtctgatt gtagtcgagg taatccagca cctgcgtaag
      541 cgccacgatg cgataggtgc ctactttgga ataggcctgg gattccttat cgaggatctc
      601 ctgttgggtg gccgatgtgc cgatctctac gaattcgata tatcgttcca ccgtgttgcg
      661 gtagttaggc gaaaagggtt tgatgatcca gtggatggcc tggagaacgg ccctcgttcc
      721 ctttttttgc agcatcttgt ctaccagctc tgccagccat ttggatttga tggcagtctt
      781 caggaaatag tccggggcat cgctgacggt gaagatatgc gaatgcgcgt tggtgaagag
      841 gttagtgggg ttgttcggca taagcaggag tgggggttga aggcggtgaa ttacgattcg
      901 ggtttcttgc cggctcttct gatatttact aattttaata agaggtcgaa ccagaagggg
      961 gcgcccgcgc tgatggagaa ggcggtgatc gcgatgccca ggacatacca gaagagcagg
     1021 gcggcgatcg ttccgaaccg gtcccaggaa gaagcctgtt cccagtgccg tttcgcttcc
     1081 ggggtacgcg gccgcaggtt caggggcgga atgccgttat gccagccgat cgggatattg
     1141 aggccggcca ggtcgtcgag gatgtcggat gcggtttgga gattggcgat ccgggtggaa
     1201 tcccggtgtt gttgtaaact atcgatctct ttcaggtcgc ggagaatgcc gtgcagcgcg
     1261 gcatagctcg agtccttgcc ggggtgtttc aggctgctgg ccgctacggc attgtacact
     1321 tgttgaaggg ctgtgagggt ggtgtctgtt tttgccttgg cgaggttgtc ggccactact
     1381 tcggctaccg gcagcaattg actgcgtagg gtggggtggg tgtagaggtt tttgaagaga
     1441 tggatactgt ctacgttcaa gcccacggcg acgaggaggg agatgatgag gaggcgtttg
     1501 cgcatgaggt ctttaaacca gccgctggtc cggtccatct ggtcgttgta ccattgctgg
     1561 atcatgccgt cgagttgcgc cagtcgttgt tcgagctcct tcccgctgag cgaagcgctc
     1621 ttttccacca tattcataag catcagcttc aggtccgtat gtttcatgtt gttcacgcct
     1681 gcctggaaca ggaggaaggg gtcggtaatc gcgccttttc cggggtcgtt ctgcagggcg
     1741 atggcgtttg ccgcggcgtc gaatttatag gagcggccat aattcccgat aacatcgatc
     1801 agcacctggc tgaacatgtt gttgctgata tattggggca ggctgttggt gtctttgcgg
     1861 atattggtga tcatcgggtg gctgtagagc agctggccga agttgacgtt caggccgtca
     1921 tcgaacatcc gggaaataac tatatacaat tgttgccccc gctcgttgaa atagctgttg
     1981 accacttccg tgagggtgct gacgagcagg ctcaatagag caaaggcgag gatgagacaa
     2041 ataatggtct cgaatagcgg cgaattggtg agggatgaca gcatatttac tgaaattacg
     2101 acgctgccga cctaaaggca agggtataaa cacctgattt cggcctttcg tgcccgcgga
     2161 ccccggcctg ccctgccctc ccaggatccg cctcttcttc tctcaatccg tccacttgcc
     2221 tcgatcagat tccgttcgag cgaataggca tactgtttgc actctcgctt cgccgggcga
     2281 ccaggccaaa atcgcaaaat attgtaaacc aataccgtat aaaatgcaaa taatccaaag
     2341 tatccagaac agaatccacg aagtccgcgg cgaaagagta ttgttagaca aggacttagc
     2401 agccctttac gagacggaga caaaggccct caaccttgcc gtaaagcgca atttgaagcg
     2461 atttcccaag gatttcatgt tccagctcac gaaggaagaa tgggatactt tgaggttgca
     2521 aacggaaacc tcaaaccact tccaggctcc cgccaattca accgaacctt tgaggttgca
     2581 gattgaaacc tcaaaatccg gccgcggcgg cacccgctac cttccctatg cctttacaga
     2641 acaaggagtt gcgatgctca gtggcatcct cagctccgat aaggcgataa acatgaacat
     2701 cgccatcatg cgcgcattcg tcgaagtccg ccgcatcatt taccagcaaa gtgatctcaa
     2761 gctacaactc caggagctca aagaccatat cggcgcccac gacgtccagc tcaaccagat
     2821 ctacgacgcc atggaaaacc tcctcgacga aaaggcgagc caacgcaaat gggacgaccg
     2881 cgaccgcatc ggtttcaggc agcgctaagt tgacgcagtg ctcatccacc gtcgtccgcc
     2941 caccaccagg cctacctaac tatgatcaat agatgtaaat actaaaccca ctgcaatcag
     3001 gcggcaacga accggcctcg tcgaaccggc cgcaagaagt ccccatcccg gacggaccat
     3061 aaaatactgc cccctccccc cgtcttttca cggtatttcc cccaacccct tgcattcccg
     3121 ccacaaaccg cttaattttc catcaaaccc ccggcatgga caaagccttt gtcagcattc
     3181 tcaccggcat ctcgccgctc gtcttcgcct atgtattcaa acggatggaa agcaggagcg
     3241 cccttgctat caataaaggc aaattagagg aggcatccaa caggatcgta ttcgccgaaa
     3301 aatacctgca gttgcaggca aaggtcctca gtgataatga attgtcggtc ctgaacaaag
     3361 aagtttccgc cgatctgttc agactcaaac aagacatcga caccctcttt atcaaaaccg
     3421 aaaaagccca taacttaaag gtcttctccg ttcaaaaact cttcctcacc ttccggcccg
     3481 cctcctggct ggggtgggtc ctggcgttcc tgttctacct ctccctcatg ttcctcgcct
     3541 tttatacgat gggcctgttt atcgacgaca attataattt ctcgttcgat gcattcaaga
     3601 aacaattgca agatggggac acgatcgcgg gctttgtcat ctgcctgtcg ctcgccctcc
     3661 tgatccggta cctggccgtc tccgcgtata agcggaaaac cgctcacgcg cttgcccatc
     3721 catagcaaag ccaacctccc cacgccccgc tggcacgatt tttttatcct ccaccccgtc
     3781 atgcggcatc cgatccttag caaaggcaag ctcctgcttt tcacctgcat catcctgata
     3841 gcctcccggg ccatgtccca ggacggtaaa gccttattcc aaaccaattg cgcccagtgc
     3901 cacaacccga tcaa
//
LOCUS       DATGFG010000714         4959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_351869, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000714 DATGFG010000000
VERSION     DATGFG010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351869"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VKQ52_13350"
     CDS             <1..566
                     /locus_tag="VKQ52_13350"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLZ88232.1"
                     /translation="GHKYVGKGKRRHRVPIYESYTGHHIRKGTETFIWKADRAGAKGG
                     AISERGEGAETDSTGEAESDSLAFDMNSPEARMRAQLYEKKYFGNSALFATLVEDEFA
                     KAGRESEGVFQRDVGIRVLQATGMPSVLIETGFLSNVDEEQYLMSEDGQSEVARNIMD
                     ALRRYKATLEGHSNGAGTEPPTGGPNR"
     gene            749..1744
                     /locus_tag="VKQ52_13355"
     CDS             749..1744
                     /locus_tag="VKQ52_13355"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLZ88233.1"
                     /translation="MKISNETKIGVMAVIALAALILGFNYLKGSSLFEHTKKLYAVFD
                     NVEGLEISNVVQINGLNIGSITAINESDKDLSQGVIITITLKKDVHIPDNSVAVINKD
                     LLSSATIVINKGSSNKYLESGDTLETKNKANLFAQVQANIDPVILKLGGTLGSLDSLI
                     QVVGTMFDPRTKSNFSAIVANLAVSSAELQKLLNAQTGALAQSLKNLQEFTGNLSKNN
                     QHISNTMDNVDKTTAHLASAKIPEAVDGLNATLSDLKGTIQRLNSTNGSLGLLINDKR
                     LYTNLEATTRSLNTLLDDFRVHPKRYVNISVFGKKDKTGPLMSPIIDSTSKPVNK"
     gene            1754..4330
                     /locus_tag="VKQ52_13360"
     CDS             1754..4330
                     /locus_tag="VKQ52_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HLZ88234.1"
                     /translation="MKPLIVVAALLGCFLLSIGARAQMGRVEFRAPGNDSVTAIDILN
                     GDTYKFVQLNPVATSTTLIGHVAIRHEKTLIYCDTMVMYPQANYIDCYGHVHINDNDS
                     VNIYSDYMKYLVDTQIVQFRHNVKLTDGKSVLTTPVLDYDLKQKVGIYEQGGRIVNKD
                     AILTSDRGTYYENTKDIHFHNHVVLNDPQYDLSADSLLYNTQTQVSTFITETFIQFKD
                     TTHRTVRTREGWYDLKNKKAQFGKRPVMTEGSQKLTGDSVRMDDSTGLAIAIGNAIYT
                     DTAQGIKLLANYMINDKKKNTFLATQRPLMIIKQDKDSLYVTADTLLSARLIDFEAMQ
                     KRLAHEDSLHRIYTDSLEKAQADSLHKAALAKAAKDSIAAANAPAVDTTAGMQLKDVS
                     DSLRDGQEDSLRIGLDSLHRLQADTTGGGRPRGPGALTDTTGKKHLTGADSAKAARPP
                     TEKELKRQQRAKEKAARKAIRDSISDLKEKIREQADSVKARQKAIKDSLLIRQQDIAD
                     SIAEVKSRERARKRFIIDSTRQAAVSDSLKIIAHADSIRHVNFIDSLERVGLTDSANA
                     IRRQDSIKLAARTRPRPPKDTAEAPLPGHEADSIGRIPTKDTTLRFVMGFHHVRIFSD
                     SLQAVADSLYYSAKDSIFRLFYNPIAWSNGNYQITGDTMYVYTKNKKAQRLYVFENGL
                     AINKVGKQFYNQLKGTTINTFFKGGEVDYIRAKGNAESIYYVQDEKKAYTGVNKAHAD
                     IIDMMFAPKFDSAGKPNGKELNRVVLRNDAEGTMYPFKHVVFEDMILRGFKWQEKLRP
                     KSKKELYEEVKRKQEEDFDDTGTPVATPAPPPAPPAQPATAPPAKTTPAQPATTTTPT
                     KH"
     gene            4452..4913
                     /locus_tag="VKQ52_13365"
     CDS             4452..4913
                     /locus_tag="VKQ52_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88235.1"
                     /translation="MRTITLCILLLVISSCINRLSAQDYKLAMGIRFSSASPTLSNSI
                     SVKYFMDESNALEGLISFGTRFGLGGLYERHQLIGGTPAFTWFWGFGGYVGFQNNNTY
                     LGPTGAIGLDYKFANAPLNLSLDWKPELDILPNINFVPDAFGITARYTFGK"
BASE COUNT         1331 a         1669 c         1145 g          814 t
ORIGIN      
        1 tcggccataa atatgtaggc aaaggcaaga gacggcatcg ggtacccatt tacgaatcct
       61 acaccgggca ccatatccgc aaaggcacgg agaccttcat ctggaaggcg gacagggccg
      121 gcgcaaaggg cggcgccatc agcgaaagag gcgagggtgc agaaacagac agcacaggtg
      181 aagccgaatc cgatagcctc gccttcgaca tgaactcccc cgaagcaagg atgcgtgccc
      241 agctctacga aaagaagtac ttcggcaaca gcgccctctt cgccaccctc gtggaagacg
      301 agttcgcaaa agccggccgc gaaagcgaag gcgtcttcca gcgcgacgtc ggcatccgcg
      361 tcctccaggc gacgggcatg cccagcgtcc tcatcgagac cggcttcctg tccaatgtgg
      421 acgaagaaca atatctaatg agcgaggacg gccagagcga ggtggcccga aacatcatgg
      481 acgcactccg ccggtataag gcaaccctcg aaggccattc caacggcgcg ggaaccgagc
      541 cacccaccgg cggccccaat cggtaaattc gagttaaacc aggccgaacc accctttcgg
      601 aataggtcct tcagccaaat attctatatt tgcggcagaa gatgcgtgcc ggaggcaagc
      661 tgctaccggc aattgaataa atacaaattc cgaatgacac gagccgaagg cgaactgcat
      721 tcggcctttg aaaaatcgta aattccgaat gaaaatttct aatgaaacaa agattggtgt
      781 gatggccgtc atcgccctcg ccgcactgat cctcggcttt aattacctca aaggcagcag
      841 cctcttcgaa cataccaaaa aactctacgc cgtattcgac aacgtcgaag gcctcgagat
      901 ctccaacgtc gtccagatca acggcctaaa tatcggcagc atcaccgcca taaacgagtc
      961 cgataaagac ctctcccagg gcgtcatcat caccatcacc ctcaaaaagg acgtccacat
     1021 ccccgacaac tccgtcgccg ttatcaacaa agacctcctc tcctccgcca ccatcgtgat
     1081 caataaagga agcagcaaca agtacctgga aagcggcgat accctcgaga caaaaaacaa
     1141 ggccaacctc ttcgcccagg tacaggcgaa catcgacccc gtcatcctga aactgggcgg
     1201 caccctcggc tccctcgaca gcctcatcca ggtggtcgga accatgttcg acccaaggac
     1261 aaagagtaat ttcagcgcca tcgttgccaa cctggccgtt tcctcggccg aattgcaaaa
     1321 attactcaat gcccagaccg gcgccctcgc ccagtcgttg aaaaatctcc aggaattcac
     1381 cggcaatctg agcaagaaca accagcacat ctccaacacc atggataatg tggacaagac
     1441 cacggcccac ctcgcctccg ccaagatccc cgaagccgtc gacggtctca acgccaccct
     1501 cagcgacctc aaaggcacca tccagcggct caacagcacg aacggctccc tcggtctgct
     1561 gatcaatgac aaacgcctct acaccaacct ggaagccact acccgcagcc tcaataccct
     1621 gctggacgac ttccgggtcc accccaaacg ctatgtgaat atctccgtat tcggtaaaaa
     1681 agacaagacc ggaccgctga tgtcccccat catcgattcc acgtccaaac ccgtcaataa
     1741 ataaggatta aggatgaagc ccctcattgt cgtcgccgcc ctcctgggct gttttctgct
     1801 gagcatcggc gcccgggcac agatgggtag ggtcgaattc agagcgcccg gcaatgacag
     1861 cgtcaccgcc atcgacatcc tcaacgggga cacctacaag ttcgtccagc tgaaccccgt
     1921 cgccaccagc accaccctca tcggccatgt cgccatccgc catgagaaga ccctcatcta
     1981 ctgcgacacc atggtcatgt acccccaggc gaactacatc gactgctatg gtcatgtcca
     2041 tatcaacgac aacgactcgg tgaacatcta ctccgactat atgaaatacc tggtcgacac
     2101 ccagatcgtc cagttcaggc acaacgtaaa actcacagac ggcaaaagcg tcctcaccac
     2161 cccggtgctg gactacgatc tcaaacaaaa ggtcggcatc tacgaacagg gcggccgaat
     2221 cgtcaacaag gatgcgatcc tcaccagtga caggggaaca tactacgaga atacaaagga
     2281 catccatttt cacaaccatg tcgtcctgaa cgacccccaa tacgatctct ccgcggattc
     2341 cctgctctat aatacccaga cccaggtctc gacattcata accgaaacct ttatccagtt
     2401 caaggacacc acccatcgca ccgtccgcac ccgcgaaggc tggtacgatc tcaaaaataa
     2461 aaaagcccag ttcggcaaac ggcccgtcat gaccgaaggc agccagaaac tcacgggcga
     2521 cagcgtccgt atggacgaca gcaccggcct ggccatagcc atcggcaacg ccatctatac
     2581 cgacacggcc cagggcatca agctgctggc caactacatg atcaacgaca agaagaagaa
     2641 caccttcctg gcgacacagc ggccgctgat gatcatcaag caggacaagg actccctcta
     2701 cgtcaccgcc gacaccctcc tatcggcccg gctcatcgac ttcgaagcca tgcaaaaacg
     2761 gctggcgcac gaggacagcc tccaccgcat ctataccgac agcctggaaa aggcacaggc
     2821 cgacagcctc cacaaggccg ccctcgcaaa agcggccaaa gactctattg ccgcggcaaa
     2881 tgcccccgcc gtcgacacga cggccggcat gcagctgaag gacgtcagcg acagcctccg
     2941 cgacggccag gaggatagcc tcaggatcgg cctcgacagc ctgcaccgcc tccaggccga
     3001 caccacggga ggaggcagac cccgcggccc cggcgccctg accgatacca ccggcaaaaa
     3061 gcacctcacc ggcgccgaca gtgcaaaggc cgcccgccca cccaccgaaa aggaattgaa
     3121 acgacagcaa cgggcaaagg aaaaagcggc ccgcaaagcc atccgcgact cgatctccga
     3181 cctgaaggag aagatccgcg agcaggccga tagcgtaaaa gcccggcaaa aagccataaa
     3241 ggacagcctc ctcatccggc aacaggacat cgccgacagc atcgccgagg tcaaatcccg
     3301 cgaacgggcc cgaaaacggt tcatcatcga cagcacccgg caggccgccg tcagcgacag
     3361 cctgaagatc atcgcccacg cggacagcat ccggcatgtc aatttcatag atagtctcga
     3421 acgggtcggg ctcacggaca gcgccaatgc catccgacga caggacagta taaaactggc
     3481 cgcacgaacg cgaccacggc cgccaaaaga caccgctgaa gcccccctgc ccggccatga
     3541 ggccgactcc attggacgaa ttcctacaaa ggacacgacc ctgcgctttg tcatgggctt
     3601 ccaccatgtc cgcatcttct cggattccct gcaggccgtg gccgacagtc tttattattc
     3661 ggcgaaggac tccatcttcc ggctcttcta taatcccatt gcctggagca acggtaatta
     3721 ccagatcacc ggcgacacca tgtatgtcta tacgaagaac aaaaaggccc agcggctcta
     3781 tgtcttcgag aatggcctgg ccatcaacaa ggtgggaaag caattctata accagttaaa
     3841 gggaaccacg atcaacacct tcttcaaggg aggggaggtc gactatatcc gggccaaggg
     3901 caatgcggaa agcatctact atgtccagga cgagaaaaag gcctacaccg gggtcaacaa
     3961 agcccacgcc gacatcatcg atatgatgtt cgccccgaag ttcgactcgg cgggaaaacc
     4021 aaacggcaag gaattgaacc gggtcgtcct gcgcaacgac gccgaaggaa caatgtatcc
     4081 gttcaaacat gtcgtgttcg aggacatgat cctgcgtggg ttcaaatggc aggagaaact
     4141 tcggccaaaa agcaagaagg agctgtacga ggaagtaaaa cgcaaacagg aagaagactt
     4201 cgatgacacc ggtacgcccg tggccacccc ggcgccgcca cccgcgccac ccgcgcagcc
     4261 cgcaaccgct ccgccggcaa agaccacacc tgcccagccg gcaaccacaa cgacccctac
     4321 aaaacattaa caacccttta tcgcttcatc ccggccctcc tcttaaacca tttactttaa
     4381 gaaacttcta acaaccgtta gtcaatattg ccggcaccgg caacgttcct tcgggataaa
     4441 acttaaccac tatgcgcacc atcacccttt gcatcctcct gctggtgatc agcagctgta
     4501 tcaatcgtct gtccgcacag gattacaaat tggcgatggg tatccgcttt agttccgcct
     4561 cccccaccct cagcaactcc atcagcgtaa aatattttat ggatgaatcg aacgccctcg
     4621 aaggcctcat ctccttcggc acccgtttcg gcctcggcgg tctttacgaa cgtcaccagc
     4681 tcatcggcgg aacccccgcc ttcacctggt tctggggctt cggcggctat gtcggctttc
     4741 aaaataacaa cacctacctc ggccccaccg gcgccatcgg cctcgactac aaattcgcca
     4801 acgcccccct caacctctcc ctcgactgga aacccgaact cgacatcctc cccaatatca
     4861 acttcgtccc cgatgccttc ggcatcaccg cccgctacac cttcggaaaa taatcgtccc
     4921 cccgtccccc caatcccgcc cgtcctccac ttcctcccc
//
LOCUS       DATGFG010000715         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1038915, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000715 DATGFG010000000
VERSION     DATGFG010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038915"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..2252
                     /locus_tag="VKQ52_13370"
     CDS             312..2252
                     /locus_tag="VKQ52_13370"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HLZ88236.1"
                     /translation="MNVSFKRASAAGFLIALGIIYGDIGTSPLYTLQTLFNEGGEINK
                     SMVFGAISCIFWTLTLQTTFKYVIITLRADNHGEGGVFSLYALVRRYGKFLVYPAIIG
                     AGTLLADGIITPPITVTSAIEGLNMVKGFGQHIVPGNNLVVEIVLVILLLLFIFQRFG
                     TKVVGGSFGPIMFLWFAMLAFAGIRQVIQYPAIFASLNPIYGIRLLTEHPHGFWLLGS
                     IFLCTTGAEALYSDLGHCGRPNIYVSWTFVKIALVLNYLGQGAWVLLRHQPDLGGINP
                     FFAIVPEKFLLVSVIISAIASIIASQALISGSFTLISEAISLGFWPRVKVKYPTEIRG
                     QIYIPSINWILFVGCVGVVLYFRTSEAMTAAYGFSITVAMLMTTFLVFYFLRYIKKWP
                     FWVVGAIVTVFVIVETSFFVANAVKLLKRLFFLVFEIGLIGTMYIWFNARKITLRLLK
                     FCDLTPRLPDLEKLSNDTTVQKYSKHLVYLTRASHHEIETRILDSIFGDPPKRADIYW
                     LVNFERVDDPYTVQYDVEMLVPAKVIRVNFHLGFRIQPRIGIMLRKVAEEMAADGEIP
                     MAGRPKDFKFVILERFLSYDNEFSTRDGFILNSYFSLLRLARSDSRAYGIDIDQAIIE
                     KVPLVVTPISKIALKRVHRPRH"
BASE COUNT          498 a          768 c          558 g          552 t
ORIGIN      
        1 ttcgccgccg caaaatcctg cgcctgcaag cagcccatcc accccaccac cccttcggcg
       61 ttgccaaaac ctgccggggc gatcccctgg ctcctcatcc ggtgaaaaag aagttccctg
      121 tttttcatga tcggtgttta ttattcaaat ttcggtaaaa ctaaaagcgg gtagtgacaa
      181 cagtatgtca gttgtcaaag gcagctttaa aaattagcgg aaatggggat atacccgcct
      241 gatatttgtt aattaaaaat atttccaccc cctttcgaac ctcatgcgcc gatttatggt
      301 aatttgaggg tatgaacgtt agcttcaaac gggcatcggc tgcgggcttc ctcatcgccc
      361 tcggcattat ctatggagac atcgggacat cccccctgta caccctccaa accctgttca
      421 acgaaggcgg tgaaataaat aagtcgatgg tattcggcgc tatctcctgt atcttctgga
      481 ccctcaccct gcaaaccacg ttcaaatacg tcatcatcac ccttcgcgcg gacaaccacg
      541 gggaaggcgg ggtattctcg ctttacgcgc tggtccgccg ctatggcaaa ttcctggtct
      601 atcccgccat catcggcgcg ggtacgctgc ttgccgacgg catcataacc ccaccgatca
      661 ccgttacctc tgccatcgaa ggcctcaata tggtaaaggg ctttggccag cacatcgtgc
      721 ccgggaacaa cctcgtggtc gagatcgtcc tggtcatcct gctcctcctc ttcatcttcc
      781 agcgtttcgg cacaaaggtc gtaggcggca gcttcggccc catcatgttc ctctggtttg
      841 cgatgctcgc cttcgccggc atccgccagg tcatccaata ccccgcgatc ttcgcctcgc
      901 tgaaccccat ttatgggatc aggctcctga ccgaacaccc ccacggcttc tggctgctgg
      961 gctccatctt tctctgtacg acaggagcgg aagccctcta ttccgacctg ggtcattgcg
     1021 gccggccaaa catctatgtc agctggacct ttgtaaagat cgccctggtg ctcaactacc
     1081 tcggacaggg cgcctgggtg ctcctccgtc accagcccga tctcggcggc atcaacccct
     1141 tctttgccat cgtcccggaa aaattcctgc tcgtcagcgt catcatttcg gccatcgcat
     1201 ccatcatcgc cagccaggcc ctgatcagcg ggtcgttcac cctcatcagc gaagccatca
     1261 gcctgggctt ctggccaaga gtgaaggtga aataccccac agagatccgg ggacagatct
     1321 atatccccag catcaactgg atcctcttcg tcggctgcgt cggcgtcgta ctctatttcc
     1381 ggacgagcga ggccatgacg gcggcttatg gcttcagcat caccgtggcg atgctgatga
     1441 cgacattcct tgtattctac ttcctccggt acataaaaaa atggcccttc tgggtcgtcg
     1501 gggccatcgt caccgtcttt gtgatcgtcg aaacttcctt cttcgtggcg aatgccgtaa
     1561 agctgttgaa acggctcttc ttcctggtct tcgagatcgg gctcatcgga accatgtata
     1621 tctggttcaa cgcccgtaag atcactctcc ggctccttaa attctgcgat ctcacgcccc
     1681 ggctgcccga cctggagaag ctaagcaatg acaccaccgt gcaaaaatac tccaagcatc
     1741 tcgtctatct gacaagggct tcgcaccatg aaatagaaac gcggatcctc gactcgatct
     1801 tcggcgaccc gccaaaacgt gccgatatct attggttagt gaacttcgaa cgcgtggacg
     1861 acccctacac cgtccagtac gatgtggaaa tgctcgtgcc cgcaaaagtg ataagggtaa
     1921 atttccacct gggcttccgc atccagcccc gcatcggcat catgctccgc aaggtggcgg
     1981 aagaaatggc cgcggacggc gagatcccca tggccggccg ccccaaggat tttaaatttg
     2041 tgatcctcga acgcttcctc tcttacgaca acgagttctc cacccgggac ggcttcatcc
     2101 tcaacagcta tttcagcctc ctccgcctcg cccgctccga cagccgcgcc tacggcatcg
     2161 acatcgacca ggccatcatc gaaaaggttc ccctcgtcgt cacccccatc agcaagatcg
     2221 ccctcaaacg cgtccaccgc ccccgccact agcgccggca tagggcgttt cccttcgggc
     2281 cgggctcttc ggcgtacacg gtactgcctc caatccctaa cgcactcagc tgctaccggg
     2341 aaagcttttt gattgtgctg ttatttaagg gtggat
//
LOCUS       DATGFG010000716         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_798519, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000716 DATGFG010000000
VERSION     DATGFG010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798519"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VKQ52_13375"
     CDS             complement(<1..552)
                     /locus_tag="VKQ52_13375"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLZ88237.1"
                     /translation="MTDHPQHIMTDHPQHIMTDRPQHIIHRIDQLASISEDEKGITRR
                     YGTPAALRATNTVLEWMRAAGLQTRIDAIGNLRGRWPSENPDAKTLVIASHLDTVVNA
                     GRWDGPLGVLAGLDILENITSQNQRFPFHIELIAFCDEEGVRFHTTYLGSKVVAGSFE
                     PATLHRQDEQGITLEQAITAIGGD"
     gene            complement(549..1979)
                     /locus_tag="VKQ52_13380"
     CDS             complement(549..1979)
                     /locus_tag="VKQ52_13380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ88238.1"
                     /translation="MPEDQIAIHSKRMLLPDGLREATLIIDKGIITGVINGLAMADGI
                     KWIDADDLLVMPGLIDPHVHINEPGRTDWEGFDTATRAAIAGGITMLVDMPLNSSPVT
                     TTVAAFLRKQTAATGQLHCDCGFWGGVVPGNENEIEPLIAHGVLGFKAFLTHSGIDEF
                     PNVTENMLRKVMPIIAANGLPLLVHCELETPRPPASPKHGPSNSRPSHPPTPGPPQPA
                     PGDPRSYKNYLASRPRKWEDDAIALMIRLCAEYNCRTHIVHLSSATSIEPIRRAKEKG
                     LPLTVETAPHYLYFMAENIPDGNTLFKCAPPIREAANNNRLWEALAEGVIDFIATDHS
                     PAPPALKQIATGHLPQAWGGISSLQLSLPVVWTAAEQRGFNEKHLAKWLSAAPAEFIG
                     QSTSRGKLAPGYRADLTIWDPGRSFTVNPSTLYHKHKLTPYAGEELFGVVEHTWLSGE
                     KVYDKGTILPNRGNPIRQNQEANIRS"
     gene            2022..2867
                     /gene="pucL"
                     /locus_tag="VKQ52_13385"
     CDS             2022..2867
                     /gene="pucL"
                     /locus_tag="VKQ52_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HLZ88239.1"
                     /translation="MTIRLDKNAYGKNAIHLSKVVRHPDRHEIRQIRVDVSLQGDFDA
                     VHVEGDNSKVLPTDTMKNTVYALAKDRFTASIEAFGLVLADHFLTHNPQVSGVTIGLT
                     EACWQRMSFDETEHPHSFLNGGSEKHTTTVVRDRQALVLSAGISDLLILKTTDSGFEG
                     YIRDQYTTLKETADRILSTSCDVSWVYGEMPPDPGALYAGIRENLLRTFADHKSLSVQ
                     HTLYAMGENVLEAFPVVKEITLKMPNKHHIPFNLQQFGMDNSNEVFIATEEPYGYITG
                     TVVRG"
BASE COUNT          574 a          701 c         1037 g          688 t
ORIGIN      
        1 atcgccgccg atggccgtta tggcctgttc gagggtgatg ccctgttcat cctggcggtg
       61 gagggtggcc ggctcgaagc tgccggcgac gaccttgctg ccgagatacg tggtatggaa
      121 gcggacgcct tcttcgtcgc aaaaggcgat gagttcgata tggaagggga agcgctggtt
      181 ctggctggtg atgttctcca ggatgtcgag gccggccagc acgccgaggg ggccgtccca
      241 gcggccggcg ttgacgacgg tatcgagatg ggaggcgatg acgagcgttt ttgcatccgg
      301 gttttccgat ggccatctgc cacggagatt gccgatggca tcgatacgcg tttgcaggcc
      361 tgcggcgcgc atccattcca gcacggtgtt ggtggccctg agggcggcgg gagtcccata
      421 gcggcgcgtg atgcccttct cgtcttcgct gatggaagcc agttgatcga tgcggtggat
      481 aatatgttgg gggcgatcgg tcatgatatg ttgggggtga tcagtcatga tatgttgggg
      541 gtgatcagtc atgagcggat gttggcttcc tggttttggc ggataggatt tccccggttg
      601 ggcaagatgg ttcccttgtc atagaccttt tcgccactta gccaggtgtg ctccacgaca
      661 ccaaagagct cttcgcccgc gtagggggtc agtttgtgtt tatggtatag ggtcgatggg
      721 ttaacggtga aggaccggcc gggatcccag atggtgagat cggcgcggta gccgggagcg
      781 agtttcccgc gggaggtgga ctgaccgatg aattcggcgg gcgcggcgct gagccatttg
      841 gccaggtgtt tctcgttgaa accgcgttgt tcggcggctg tccagaccac ggggagggat
      901 agttgcagag aggagatgcc gccccatgcc tgtggcagat ggccggtagc gatttgtttc
      961 agggccggcg gcgcggggct gtggtcggtg gcgataaagt cgatcacgcc ttcggctaag
     1021 gcttcccaca accggttgtt gttggcggct tccctgatgg gtggggcgca tttgaagagc
     1081 gtattgccgt cggggatatt ctcggccatg aaatagagat aatggggagc ggtctccacc
     1141 gtcaaaggca gtcctttctc ttttgcccgg cggatgggtt cgatgcttgt cgccgaggaa
     1201 aggtgaacga tatgggtgcg gcagttgtat tcggcgcaga ggcggatcat cagcgcgatg
     1261 gcgtcgtctt cccattttcg cgggcgggag gcgagatagt ttttgtatga gcgggggtcg
     1321 ccgggagcgg gttgtggcgg gccgggagtt ggggggtgtg aagggcggga gttcgaaggg
     1381 ccatgtttcg gggaagcggg cggtcggggg gtttccagtt cacagtggac cagcaggggg
     1441 agcccgttgg cggcgatgat gggcatcacc tttcgcagca tgttttccgt tacattggga
     1501 aactcgtcga tgccggaatg ggtgaggaag gccttgaagc cgaggacgcc gtgggcgata
     1561 aggggctcga tctcgttctc gttgcccggc actaccccac cccagaaccc gcaatcgcag
     1621 tgcagctggc cggtggcggc ggtctgtttc cggaggaagg cggcgacagt ggtggtgacg
     1681 ggtgaggaat tgaggggcat gtccaccagc atcgtgatcc cgccggcgat ggcggcgcgg
     1741 gtggcggtat cgaagccctc ccaatccgtg cgtcccggct cattgatatg gacatgggga
     1801 tcgatgagcc cgggcatgac aaggaggtcg tccgcgtcta tccatttgat gccgtcggcc
     1861 attgcgaggc cgttgatgac gcccgtgata atgcccttat cgatgataag cgtagcttcc
     1921 ctgagcccat cgggcaggag catccgtttg ctgtgtatgg ctatctgatc ttccggcatt
     1981 ttctataatg agaaagttaa gaaatttatt tatattggga tatgactatc cgcctggaca
     2041 aaaatgcgta tggaaagaat gcgatccacc tgtcgaaggt ggtccggcat cctgaccggc
     2101 atgagatccg gcagatcagg gtggatgtct ccctgcaggg ggatttcgat gcggtgcatg
     2161 tggagggtga taacagcaag gtgttgccca cggataccat gaagaatacg gtgtatgcgc
     2221 tggcgaagga ccggtttacg gcctcgatcg aggcgttcgg gctggtgctg gccgatcatt
     2281 ttcttactca taaccctcag gtgtcggggg tgacgatcgg gttgaccgag gcctgctggc
     2341 aacggatgtc ttttgacgaa acggaacatc cccattcctt tttgaatggc gggagcgaga
     2401 agcatacgac caccgttgtc cgggaccggc aggcgcttgt gctttccgcc gggatcagcg
     2461 atctgctgat cctgaagacg accgattcgg gttttgaagg atatatccgg gatcagtata
     2521 ctacgctgaa ggaaaccgcg gaccggattt tgtctacttc ctgtgacgtg agctgggtgt
     2581 atggggagat gccgccggac ccgggggcgc tgtatgcggg gatcagggag aatttgttgc
     2641 ggaccttcgc cgatcacaag agcctttccg tgcaacacac tttatacgcc atgggagaga
     2701 atgtattgga ggcgtttccg gtagtgaagg agatcacgct gaaaatgccg aacaagcatc
     2761 atattccttt taatcttcag cagtttggca tggataatag caatgaagtt tttatcgcga
     2821 ccgaggagcc gtatggctat atcaccggta cggtggttcg cggttgaccg tgccgccggc
     2881 gatgtgaaat gacccgtcct aaaaaagcta tacactaaaa acttagtgta tgtcattgga
     2941 aaacaggaaa aatgagttgc cggcgctcaa gaaggcgggt gggcattatg acaagttagc
//
LOCUS       DATGFG010000717         4500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1195924, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000717 DATGFG010000000
VERSION     DATGFG010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195924"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2030)
                     /locus_tag="VKQ52_13390"
     CDS             complement(<1..2030)
                     /locus_tag="VKQ52_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88240.1"
                     /translation="MKIAPFLLLLCLGALPALAQSDALGSVQTRADTWQNVVLQEKLF
                     VHTDKSFYLAGELLWCRLYCVDGNTHRPQGVSRLAYVELLDQDNKPVLQGKIALEKGF
                     GDGSFYIPPSILTGAYQLRAYTNWMKNFGPETFFTQPVTIVNTIKGVDTAAITRPDTT
                     GSHYSLTFFPEGGDLVTGLPSTLAFEAVDQQGQGLNTEGAILTGNNDTVATFRPQQEG
                     MGILRFTPSAGESYKAILYCPDGVTRTWPLPPVLSQGYTLHLREDGGQEITLIVHASP
                     GLAPQNTRLFVHSATNSAFTGQTPTVTGDSAVFTLDKSHLQAGNTRFTLFDATGRPQA
                     ERLFFIAPRNKLNITAATDKSEYTTREKVRLDITTAENSAPPLTTADNRDKPLNAADT
                     PGARLSIAVYRLDALQGYPSTDILRYLWTSSEWIGRTPTDRVFDDPTLAGLYSLTHGW
                     TRFRWTDVLSHPSGPRLYPPEIRGQFITGRLSDPHTGAPEKETTTWLSTPGFTFQFAS
                     TQTDTSGKFQFDIKDFYGSDGIVIHTDNNAKVDISTPFSERYTGARIPPFRLTRSQLR
                     LLTRHNVGMQVQNVFTGDSLKRYTPPRVDTTPFFTRADYTYLLDDYTRFTTIEEVLRE
                     YVREINITRPHGHLHLVMLNEPLHSIFNDNRTLVLLDGVPVPNDRIFSYDAL"
     gene            complement(2040..3170)
                     /locus_tag="VKQ52_13395"
     CDS             complement(2040..3170)
                     /locus_tag="VKQ52_13395"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HLZ88241.1"
                     /translation="MVTTVKIFSVFSLAIFTTMACKQTYAPPAVAHPPNYLVIEGFIE
                     NNGTDSTFFHLSRTIKADTNGYVPELGAVCTIDGSDNTSRPLGEIGNGAYAAKLTGLD
                     PDATYRLRISTSNGKQYVSDNVPLVKNPPIDSINWKRLDNDVYQGIQIYANTHDPQNN
                     THYYRWEYEETWEFHSTFFAAVKYDPATGIISPYFPNTINTCWKDVLSSRILLGTSAQ
                     LAQDLIYEAPLVLVPLNSQQINWRYSLLVKQYALTKDAFNWWQILSKNTEQIGSIFGV
                     LPAANPGNIHCLSDPAEMVLGYVSGGNTRSQRIFITIDQVQPWFYDPDCPDIKPDPSH
                     SLQSLLGAGFVPWRADQTGVFLSYPRCIDCTLSGTNIRPSFW"
     gene            complement(3164..4291)
                     /locus_tag="VKQ52_13400"
     CDS             complement(3164..4291)
                     /locus_tag="VKQ52_13400"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HLZ88242.1"
                     /translation="MRPCNAILLICLSCSIGCKQTYQPPAIANPPDYLVVEGFINTNP
                     GESTTFNLSHTIKVDTNLYTPEPGATVTVEGSDNNQYRLPEAGTGSYTAPLSGLDYNI
                     TYRLHIITAKGKQYASDYFPLRTSPPIDSISWRRLENDIHQGIQVYASTHDPQNKTHY
                     YRWDYQETWEFHSHFFATAQYVPVGHVQNYSPNTISVCWKSDQASNILLGTTKQLSQD
                     VIHEAPLALIPLNSQQISVRYSILVKQYALTDSAFNWWQIMQKNTEQLGSIFGVQPSA
                     NMGNIHCLTDPSETVLGFVGGGNVQSQRIFITIDQVRPWVYNPDCTDNTIPNNPDSIK
                     LWYDRGYLPWYVDISPPQVHVAYATCVDCTLTGSNIKPSFW"
     gene            complement(4291..>4500)
                     /locus_tag="VKQ52_13405"
     CDS             complement(4291..>4500)
                     /locus_tag="VKQ52_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88243.1"
                     /translation="FRADFAMNIDGNFKVHQKTHDSWTIGVYNLTGRKNPYNVYFVSE
                     NGAVNGYKLSIFGSAIPFINFNIHF"
BASE COUNT          890 a          967 c         1581 g         1062 t
ORIGIN      
        1 ttcagggcgt cgtaggaaaa gatgcggtcg tttggcacgg gtactccgtc gaggagcacc
       61 agtgttctgt tgtcgttaaa gatcgaatgg aggggttcgt tcagcatcac caggtgaagg
      121 tggccgtggg gccgggtaat attgatctcc ctgacgtact cgcgcagcac ttcttcgatg
      181 gtcgtaaacc gcgtgtaatc atccagcaga taggtatagt cggcccgggt aaagaatggg
      241 gtcgtgtcga cgcggggagg ggtgtagcgt ttcaggctat cgccggtgaa gacattttgc
      301 acctgcatgc cgacgttatg ccgcgtcagc aggcggagct gcgaccgggt gaggcggaag
      361 ggcgggatgc gggcgccggt atatcgctcc gagaacggcg ttgagatatc gaccttggcg
      421 ttgttatcgg tatggatgac gatcccgtcg ctgccataga aatcctttat atcgaattgg
      481 aatttgccgc tggtatccgt ttgggtggag gcgaactgga aggtgaaacc cggggtggag
      541 agccaggtgg tggtctcttt ttcgggtgcg cccgtatgcg ggtcggaaag gcggcctgtg
      601 atgaactgcc cgcggatctc gggcggatag aggcggggtc cggaggggtg ggagaggacg
      661 tccgtccagc ggaatcttgt ccagccgtgg gtaagcgaat aaagacctgc aagggtggga
      721 tcgtcgaaga cccggtctgt cggggtgcgg ccgatccatt ccgaggaagt ccagagatag
      781 cgaaggatgt ccgtagaggg atagccctgc agggcgtcga ggcggtagac tgcgatggag
      841 agccgggcgc cgggcgtgtc ggctgcgttg agaggtttgt cccggttgtc cgctgtggtg
      901 aggggggggg ctgagttttc cgctgtggtg atatcgagcc gtactttttc ccgggtggta
      961 tattccgatt tgtcggtggc tgccgttatg ttcagcttgt tgcggggggc gatgaagaag
     1021 aggcgttctg cctgtgggcg acctgttgcg tcgaaaaggg tgaagcgggt gttgccggcc
     1081 tgcagatggc ttttatcgag ggtgaagaca gccgagtcgc ctgttactgt gggcgtctgc
     1141 cccgtaaagg ccgagttggt tgccgagtga acgaagagcc gcgtgttttg aggggccagg
     1201 cccgggctgg cgtggacgat aagggtgatt tcctggccgc cgtcctcgcg gaggtgcagc
     1261 gtgtagcctt gtgagagtac cggcgggagg ggccaggtgc gggtaacacc atcggggcag
     1321 tagaggatcg ccttatagga ttcacccgcg gagggtgtga accggaggat gcccatccct
     1381 tcctgttgcg ggcgaaaggt agcgaccgtg tcgttgttgc cggtgaggat cgccccttcg
     1441 gtattcagac cctgcccctg ttggtcgacg gcttcgaagg cgagggtaga ggggaggccg
     1501 gtgacgaggt cgccgccttc ggggaagaag gtgagtgaat agtggctgcc ggtagtatcg
     1561 gggcgggtga tggcggcggt gtcgacgcct tttatcgtat tgacgatggt gaccggttgg
     1621 gtgaaaaagg tttcggggcc aaaattcttc atccagttgg tgtatgcgcg gagctggtag
     1681 gcgccggtga gaatggaggg ggggatatag aaggagccgt cgccgaagcc tttttccagc
     1741 gctatttttc cctgcaggac cggtttgttg tcctggtcca gcagttcgac ataggccagc
     1801 ctgctcacgc cctgggggcg atgtgtgttc ccatccacgc agtagagtct gcaccagagg
     1861 agctcgccgg cgagataaaa agatttatcg gtatgtacga agagcttttc ctgcaacacc
     1921 acgttttgcc aggtgtcggc gcgggtctgt acggagccga gggcatccga ttgcgcgagc
     1981 gccggaaggg cgccgaggca taagaggaga aggaagggag caattttcat gttttatttt
     2041 taccagaatg atgggcgaat attggtgccg gagagggtgc agtcgataca gcgcgggtag
     2101 gaaaggaaga cgccggtctg atctgctctc caaggcacga accccgcgcc caataagctc
     2161 tgcaagctat gactcggatc gggttttatg tccggacagt cagggtcata gaaccagggc
     2221 tgtacctgat cgatggtaat gaagatgcgt tgtgaccggg tattgccgcc gctgacataa
     2281 cccaggacca tttcagcggg gtcgcttagg caatggatat tgcctgggtt ggctgcaggc
     2341 aggacgccga agatcgaacc gatctgttcg gtgttcttcg aaaggatctg ccaccagttg
     2401 aaggcgtctt ttgtcagggc gtattgtttg accagaaggc tgtagcgcca attgatctgt
     2461 tgggagttga gcgggaccag gaccaggggg gcctcgtaga tcaggtcctg agccagctgt
     2521 gccgaggtgc ccagcagaat gcgggaggag aggacgtctt tccagcaggt gttgatcgta
     2581 ttggggaaat agggggagat gatgccggtg gcaggatcat atttgactgc ggcgaaaaaa
     2641 gtggagtgaa actcccaggt ctcctcgtac tcccagcgat aataatgtgt attgttttgg
     2701 ggatcgtggg tgttggcgta gatctggatc ccctggtaca cgtcattatc cagccgtttc
     2761 caattgatgc tgtcgatggg cggattcttc accaggggga cgttatccga tacatattgt
     2821 tttccgttgg aggtggagat gcgaagccgg taggtggcat cggggtccaa gccggtgagc
     2881 tttgcggcgt aggcgccgtt gcctatttca cccagcggcc gggacgtatt gtcggagcca
     2941 tctatcgtgc agacggcgcc cagttcaggg acatagccat tggtatcggc ctttatcgtg
     3001 cgggagagat ggaagaaggt ggagtcggtg ccgttgttct ctatgaaacc ttcgatcacg
     3061 aggtaattcg gcggatgggc gacggcgggt ggcgcatagg tttgtttgca ggccatggtt
     3121 gtgaagatgg ccagggaaaa gacagagaat attttaacgg tcgttaccaa aaggagggtt
     3181 ttatattgct gccggtgagg gtacagtcga cgcaggtcgc gtaggccaca tggacctgcg
     3241 gcggggagat atcgacatac caggggaggt agcccctgtc gtaccacaac tttatgctgt
     3301 cggggttgtt gggtattgta ttgtcggtgc agtcgggatt ataaacccag ggtctcacct
     3361 gatcgatggt gatgaagatg cgttgggatt gaacgttgcc gccgccgacg aatccaagta
     3421 ccgtttcgga agggtcggta aggcagtgaa tatttcccat attggcggag ggttgaacac
     3481 cgaagatgga acccagctgc tccgtgtttt tttgcatgat ctgccaccag ttgaaggcgg
     3541 agtccgttag cgcatattgt ttgaccagga tgctgtagcg gacgcttatc tgctgggagt
     3601 tgagggggat gagcgccagt ggggcttcat gaatgacatc ctgcgaaagc tgttttgttg
     3661 taccgaggag aatattggag gcctggtcgc tcttccagca gacggagatc gtattggggg
     3721 aataattttg tacatggcca accgggacgt attgagcggt ggcaaagaag tgggaatgga
     3781 actcccaggt ctcctggtaa tcccagcgat agtaatgggt tttgttttgc gggtcgtggg
     3841 tactggcgta gacctggatg ccctggtgga tgtcgttctc cagccgtcgc cagctgatgc
     3901 tgtcgatggg cgggctggtg cgtagcggga aataatcgga agcgtattgc tttccttttg
     3961 cggtgatgat atggagacgg taagtgatgt tatagtcgag tcctgaaagc ggggcggtgt
     4021 atgatccggt gcctgcttcg gggagcctgt actggttatt atcggagcct tcgaccgtga
     4081 cggtggcgcc cggttcggga gtatagagat tcgtatccac ttttatggtg tgggataggt
     4141 tgaaggtggt ggattcgccg gggttggtat tgatgaaccc ttccacgacg agatagtcgg
     4201 gcgggttggc gatggcggga ggctggtagg tttgtttgca tcctatcgag cagctcaggc
     4261 agataagcaa tatggcatta cagggacgca ttaaaaatga atgttgaagt tgatgaatgg
     4321 gatagcgcta ccaaagatgg agagcttgta tccgttgacc gccccgttct cagatacgaa
     4381 gtagacgttg tagggattct ttcttcccgt gagattgtat acgccgatcg tccaggagtc
     4441 gtgggtcttc tggtggacct taaagttgcc gtcgatgttc atggcgaaat cggcgcgaaa
//
LOCUS       DATGFG010000718         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_311251, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000718 DATGFG010000000
VERSION     DATGFG010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311251"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VKQ52_13410"
     CDS             <1..420
                     /locus_tag="VKQ52_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ88244.1"
                     /translation="SSVSFGGTPAASFTVMSDSVLGATVGSGSSGFVMVANVSGSDSL
                     AGFVFIDSTPSAHALLLKEYPNPAIGVLYVTVPNTTASSKLMLTDMNGKVLRTIYVAP
                     NATTVRVNVSRFVRGMYKVVWTDGKHLANQTVLVLNE"
     gene            complement(511..1506)
                     /locus_tag="VKQ52_13415"
     CDS             complement(511..1506)
                     /locus_tag="VKQ52_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88245.1"
                     /translation="MLITTVFAACGHPASQPPKPISDSACAVSTVKQLLKWYAKKYDS
                     IDAFQLVTMPPSEDSGWNTVNFDETARYLQTLRSSGFFTEKFLADKQTYFRDCDSNFA
                     VHKENNRSPTGFDIDVLLFSNNTEDLLQDTIAPNIQLTGGTVTFDKYLVFTTKKEGDT
                     CRIDNIRFVTDPDSAADAVCTRGADTTEPAIREIKREYEKIRADSSKFQVFTKDIMDE
                     STEGGDATLYFEERQLRKAVVHFFGETGKLRSEYYFVHGKMFFYIGRETSYTKPFYIA
                     NYKIGNVKVNRCYFHNGQMIRWIDERGKLVDKTLYPAKEKELLVTDSSFFTVDNP"
     gene            1794..>2590
                     /locus_tag="VKQ52_13420"
     CDS             1794..>2590
                     /locus_tag="VKQ52_13420"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HLZ88246.1"
                     /translation="MKALVYHGAGRKAWEEKPKPGLRVPTDAIVKILKTTICGTDLHI
                     MKGDVPEVTDGRIIGHEGVGIVEEAGSGVSNFKKGDHVLISCITSCGKCEYCKRGMYS
                     HCADGGWILGHLIDGTQAEYVRIPHADNSLYLIPNGADEEALVMLSDILPTGFECGVL
                     NGQVKPGDTIAIVGAGPVGLAALLTAQFYSPAEIIMIDVDDNRLNVAKTFGATQLINS
                     SDGKPVEEVMRLTGGKGVDVAIEAVGVPATFEICESIVNAGGRIANVG"
BASE COUNT          568 a          581 c          838 g          603 t
ORIGIN      
        1 agctccgtga gctttggcgg gacgccggct gcttccttta cggtgatgag tgacagtgtt
       61 ctgggtgcga cggtgggtag tggttcatcg ggctttgtaa tggtggccaa tgtttcgggc
      121 agcgattcgc ttgcgggctt cgtcttcata gattcgacgc cttccgcgca tgcattgttg
      181 ctgaaagaat accccaaccc ggccatcggc gtgttgtatg tcacggtgcc gaatacgacc
      241 gcttcgtcga aactgatgct gacggacatg aacggcaagg tgttgaggac catttatgta
      301 gcgcccaatg cgactacggt gcgcgtgaat gtcagcaggt ttgtcagggg tatgtataaa
      361 gtggtgtgga ccgatgggaa gcacctggcg aatcagacag tgttggtatt gaatgaataa
      421 ggtgatgtga aatagtatgg cggccccgct acgcggggcc gttttatttc gcggcggttg
      481 cgggtagaag caggagaagg aagcaacgct tcaggggttg tcaacagtaa aaaatgacga
      541 gtcggtgacc agcaattctt tctcctttgc ggggtagagg gtcttgtcga cgagcttgcc
      601 tctttcatcg atccatcgaa tcatttgccc gttgtgaaaa tagcagcggt tcacctttac
      661 gttgccgatc ttatagttgg caatgtaaaa aggctttgta tagctggttt cccggccgat
      721 gtagaagaac atttttccgt gtacgaaata atactcactt ctcagtttcc ccgtctcgcc
      781 aaagaagtgc actacggcct ttcgcaattg gcgctcttcg aagtagagcg tggcgtcacc
      841 gccctcggtg gactcatcca tgatatcctt tgtgaagacc tggaacttac tgctgtcggc
      901 gcggatcttt tcgtattccc tttttatttc ccggatcgcg ggttcggtgg tatcggcgcc
      961 gcgtgtgcag acggcatccg ccgccgagtc ggggtctgtc acaaaccgga tgttatcgat
     1021 gcggcaggtg tcgccttcct tcttcgtggt aaagaccagg tatttgtcga aggtgaccgt
     1081 gccgcctgtc aactggatgt tgggggcgat cgtgtcctgg agtaagtcct cggtattatt
     1141 ggagaacagg aggacatcga tgtcgaatcc tgtgggcgag cgattgttct ctttatgaac
     1201 ggcgaagttg gaatcgcaat cgcggaaata ggtctgcttg tccgccagga atttttcggt
     1261 gaagaatccg gaagatcgca gtgtctgcag gtagcgcgcg gtctcgtcga aattcaccgt
     1321 gttccacccg gagtcttcgg aaggcggcat ggttaccaat tggaaggcat cgatggagtc
     1381 atatttcttt gcataccatt tcaggagttg ttttacggtt gagacggcgc aggcgctgtc
     1441 ggatatgggt ttgggaggtt gggacgcggg gtggccgcag gcggcgaata ccgtagttat
     1501 tagcaaaaaa gcatatagga gtcgcatagg gcgataaaat aaggcaaaat gcggaggtgt
     1561 gcaaacgggg gggggtgcgt cagggatcga agcggcagcc cggagcccga gccccggcga
     1621 gggcgaggac tatagcgaag agcccggccc ggcgccgcgc cggggacgcc cagatcgtta
     1681 tgtggcggcg acggtttgga ataaaggtga cctatatcat gcggcgatgg ggggaatgtc
     1741 aggctttggg ccggggggta atcctagttt accgcgaaaa aaaatagtat aacatgaaag
     1801 cattagtata ccatggcgcg ggcaggaaag cctgggaaga aaagcccaaa ccggggctca
     1861 gggttccgac ggacgccatt gtaaagatcc tgaagacgac gatctgcgga acagacctgc
     1921 atattatgaa aggcgatgtg cccgaggtaa cggatggccg gatcatcggt catgaggggg
     1981 ttgggatagt ggaagaagcg ggtagcggcg ttagtaattt caaaaagggg gatcatgtgc
     2041 tgatctcctg tatcacctcc tgcggcaaat gcgaatattg caagagggga atgtattctc
     2101 attgtgcgga cggcgggtgg atattgggcc atcttatcga tggtacccag gcggagtacg
     2161 taaggatacc gcatgcggac aacagcctct acctgattcc caatggcgct gatgaagaag
     2221 ccctggtcat gctgagcgat atattgccta ccggtttcga atgcggtgtt ctcaacggcc
     2281 aggtaaagcc gggggacact atcgcgatcg tgggtgcggg gccggtgggg ctcgccgccc
     2341 tgctgaccgc tcaattctat tctccggccg agatcatcat gatcgatgtg gacgacaatc
     2401 gcctgaacgt ggcaaagacc tttggggcga cgcaactcat caacagcagc gatggcaagc
     2461 cggtggagga ggtgatgcga ttgaccggag ggaagggggt ggatgtggcc atcgaagcgg
     2521 tgggcgtgcc ggccactttc gagatctgtg aatcgatcgt gaatgcggga ggtcgtatcg
     2581 ccaatgtagg
//
LOCUS       DATGFG010000719         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_147061, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000719 DATGFG010000000
VERSION     DATGFG010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147061"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VKQ52_13425"
     CDS             complement(<1..1123)
                     /locus_tag="VKQ52_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88247.1"
                     /translation="MITADFTGEEGKLNTMFRECVGAGRANEGLRADWQQQLAYVRKE
                     CGFSYIRMHGLLSDDMAVYQEDRNGTPVYNFMYIDVLFDFLHSIGMKPFVELGFMPSW
                     LASGNKTIFWWRGNVTPPRDYEKWGALVQHLTQHFTDRYGADEVKSWYFEVWNEPNLD
                     GFWAGTQQDYFKLYTYSARAIKQVNPAYKVGGPATAGAAWEPELIAYCHNNNVPIDFI
                     STHSYGVKAGYLDEFGNSGTVLDKNPMSVSGDVLQSRKEIASSAMPGLELHYTEWSSS
                     YTPADPLHDSYHEAAYILQKLRQVGKAANSMSYWVFTDIFEEAGPRFTPFHGGFGLLT
                     IQGINKPAFYSYQFLNRLGDIELRNQDSSSWVCRDSAGGI"
     gene            complement(1241..>2361)
                     /locus_tag="VKQ52_13430"
     CDS             complement(1241..>2361)
                     /locus_tag="VKQ52_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HLZ88248.1"
                     /translation="QLPQIFPSYNTSPIAPDATGMGSTAAQITTRIKLGWNIWNTMEA
                     PGGETGWGNPLVTQAQIDLISHSGFNAIRIPCAWNFHADQTTAKIDTAWLSRVKQVVQ
                     YCINDGMYVLLNCHWDGGWLDCTATGAKLDTIKARQKAFWEQIATKMRDFDEHLMLAS
                     ANEPNATDLPTATVLENYHQVFINAVRSTGGKNTFRTLVIQAPSTSVDLADYFTAVPG
                     MPVDQVPDKLMLEVHWYSPATFCILSADASWGAECRYWGTNYHSTDDLARNAAPGTEE
                     SYVDSSFNYVNTRFVKNNIPVIIGEFGVANHAVNILGNPADSVLSLNSRAHFFRHVVQ
                     QARNNGIPAFLWAGVFDRASNTINDPQALDSLKAGAGL"
BASE COUNT          495 a          623 c          671 g          572 t
ORIGIN      
        1 ggatgccgcc ggcggaatcc cggcagaccc aggaggaaga atcctggttc ctgagctcga
       61 tatcgcccag cctgttgagg aactggtaag aatagaatgc cggtttgttg atcccctgga
      121 tggtgaggag gccgaagccg ccgtgaaatg gtgtgaagcg gggtccagcc tcttcgaaaa
      181 tgtcggtgaa gacccaatac gacatagaat tggccgcctt gcctacctgc ctgagctttt
      241 gcaggatata ggcggcttcg tggtagctgt catgcagcgg atcggccggg gtataggagg
      301 agctccattc tgtgtaatgg agttcgagac cgggcatcgc agaggaggcg atctccttgc
      361 gggactggag tacgtccccg ctgacgctca tggggttctt atcgaggacg gtgccggagt
      421 tgccgaactc atcgaggtag cctgccttga caccatagga atgggtgctt atgaagtcga
      481 tgggaacatt gttattgtgg caataagcga tgagctccgg ttcccacgcg gcgcctgccg
      541 ttgcggggcc gccgaccttg taagcagggt ttacctgctt gatggccctg gcgctatagg
      601 tgtatagttt gaaatagtcc tgctgggtgc cggcccaaaa accatccagg ttgggctcgt
      661 tccaaacctc gaaataccag ctcttcacct cgtcggcgcc gtaccggtcg gtaaaatgct
      721 gcgtgaggtg ttgcaccagc gcgccccatt tttcatagtc cctggggggc gttacgtttc
      781 cccgccacca gaagatggtc ttgttgccgc tggcgagcca tgatggcatg aagcccagtt
      841 cgacaaaggg cttcatgccg atgctgtgca aaaaatcgaa taaaacatcg atgtacatga
      901 aattataaac cggcgttcca ttcctgtctt cctgataaac ggccatatcg tcggataata
      961 gcccgtgcat acggatatag ctgaatccgc attcttttct gacataggcc agttgctgtt
     1021 gccagtcggc cctgaggcct tcgttggcgc ggccggcgcc cacgcattcc cggaacatgg
     1081 tgttcaattt cccttcctcg ccggtgaagt cggccgttat cacgcgttgc gggattattg
     1141 atgcggcggg tttcgatctt tgggcactgg taatccccgg gtgcaataag ataagcattg
     1201 ctgcgaggca tagattattt cgcatagtat attttctgaa ttagagtccg gcgccggctt
     1261 ttaaagagtc caatgcctgc ggatcattta tggtgttact tgccctgtcg aacacgcctg
     1321 cccacaagaa tgcggggatc ccgttattgc gggcctgctg caccacgtgc cggaagaaat
     1381 gggccctgga attcagggac aacaccgaat ccgccgggtt tcccaatata ttcaccgcat
     1441 ggttcgccac gccgaattca ccgatgataa cagggatatt gtttttcacg aatcgcgtgt
     1501 ttacataatt gaacgaactg tccacataag attcttccgt cccgggcgcc gcatttctcg
     1561 ccaggtcgtc ggtggaatga taattggttc cccaataccg acactccgcc ccccagctgg
     1621 cgtcagccga cagtatgcag aaggtggcag gggaatacca gtgcacttcc agcatcagct
     1681 tatccggcac ctgatccacc ggcattcccg gcacggcggt gaaataatca gccagatcaa
     1741 cagatgtact gggcgcctgg ataaccaacg tgcggaatgt attctttccc cctgtggagc
     1801 gaaccgcgtt gataaatacc tggtgatagt tctccagcac cgtggcagtc ggcagatcgg
     1861 tggcattcgg ctcattggcg cttgccagca tcaggtgttc atcaaaatcc cgcatttttg
     1921 tagctatctg ctcccaaaat gctttttgcc tggcttttat ggtatccagt ttcgccccgg
     1981 tagcggtgca atccaaccat ccgccgtccc agtggcaatt gagcagtaca tacatgccgt
     2041 cattgataca atattggacg acttgtttta ccctgctgag ccaggcggta tcgatcttag
     2101 cggttgtttg atccgcgtgg aagttccatg cgcagggaat gcggatagca ttaaagccgc
     2161 tgtgactgat cagatcgatt tgcgcctggg tgaccaacgg gtttccccag ccggtttcgc
     2221 cgcccggggc ctccatggtg ttccagatat tccagcccaa tttgatcctg gtggttatct
     2281 gggccgcggt gctgcccatg ccggtggcgt cgggagcaat gggcgaggta ttataagatg
     2341 ggaagatctg gggaagctgg a
//
LOCUS       DATGFG010000720         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1526349, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000720 DATGFG010000000
VERSION     DATGFG010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526349"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VKQ52_13435"
     CDS             complement(<1..248)
                     /locus_tag="VKQ52_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88249.1"
                     /translation="MKIQREVVLPGKGQSFRLFSPSLRDHFYWHYHPEYELVYVEATA
                     GIRHVGLHVSSYVESDLVLIGPNIPHLNFDYGLRTEYH"
     gene            388..1356
                     /locus_tag="VKQ52_13440"
     CDS             388..1356
                     /locus_tag="VKQ52_13440"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLZ88250.1"
                     /translation="MQTTMATTTLPDNAHKDIPGNPSTATSSRVQLRRTLDGPALKVL
                     TEADWAFWRHNGYVIVRNAVSREQVAETARFLWEFEEKDPGNPETWYTPPRAEMQMKE
                     LTNTGMVEVYNHQQLWNNRQMPRVHAAFADIWGREDLWVTIDRANLNFPLRPGFEYKG
                     FIHWDYDPETKPQNVQGVLALADQTDENMGGFQCLPELFRTYDSWKLTQPENRDHFKP
                     DITGFQLEKVKLEAGDLLIFNSSQPHGIRPNLTKDKVRIAQYISMMPAEEDNEPLRQW
                     RIRSWQDRIAPEGYAFPGDPRNWEQTRYTTAVLSPLGRKLLGLDRW"
     gene            1482..>2377
                     /locus_tag="VKQ52_13445"
     CDS             1482..>2377
                     /locus_tag="VKQ52_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88251.1"
                     /translation="MKLSPLISAALAATALLAASCGSSRQAAADPGPTTWQNAPITVD
                     GSDKDWTKPLPYAISAEKIAYSVTNDDQNLYILLSTKNPQEQQKIIQGGMTIWINTKA
                     DKSESEAVGIGYPLDSRNDHDRQLMEQAQPDRYKNNKPITLEDKKDYALYGFDKSSAV
                     GNYTYGEDNPQGVKMRLDFNNEGELIYEASIPLTTLYPNHNPSASYAANSVAIGIFIE
                     GLPPDAHVPRGNGGGGPEIGVGGGLGFGSFGSGGGIGISIGTGSLIGGGGRNKQLFRE
                     TQTWQVVQLARGHSAKLDHPTNP"
BASE COUNT          589 a          825 c          566 g          397 t
ORIGIN      
        1 tggtggtact ccgtccgtag cccatagtca aaattgagat gcgggatatt gggcccgatc
       61 agcaccaggt cgctctccac ataactcgac acatgcaagc cgacatgccg gataccggcc
      121 gtcgcctcga catagactaa ctcgtactcg ggatgataat gccagtaaaa atggtcgcga
      181 agactgggtg agaacagccg gaagctctgg cccttccccg gcaaaaccac ctcccgttgg
      241 atcttcataa aaaggcaaat ttcttcccta aagctactat tcacccttca ataggcaaaa
      301 aacagtcaat ggagagcaaa aactggtcaa ccaggcggtt gcggtcccgg actccgattg
      361 ggaactttgg gtcataaaaa ttgtcatatg caaacgacca tggctaccac gactcttccc
      421 gacaacgctc ataaggacat cccgggtaac ccttccaccg ccaccagcag cagggtccag
      481 ctccgccgga ccctcgacgg tccggcgcta aaagtcctca ccgaagccga ctgggcattc
      541 tggcgacaca acggctatgt catcgtcagg aacgccgttt cccgcgaaca ggtggcggaa
      601 accgcgcgct tcctctggga attcgaagaa aaagacccgg gtaaccccga gacctggtac
      661 acgccgcccc gtgcagaaat gcagatgaag gaactcacca acaccggcat ggtcgaggtc
      721 tataatcacc agcagctctg gaacaaccgc cagatgcccc gcgtccatgc agccttcgcc
      781 gatatctggg gcagggagga tctctgggtc acgatcgacc gcgccaacct caattttccc
      841 ctccgcccgg gcttcgaata caaagggttt atccactggg actacgaccc cgaaacaaaa
      901 ccgcaaaacg tccagggcgt cctcgccctc gccgaccaga ccgacgagaa catgggcggc
      961 ttccaatgcc tccccgagct cttccgcacc tatgacagct ggaaactcac ccagcccgaa
     1021 aaccgggatc acttcaaacc cgacatcacc ggcttccagc tggaaaaggt caaactcgaa
     1081 gccggcgacc tgctcatctt caacagctcc caaccccatg gcatccgccc caatctcaca
     1141 aaagacaagg tccgcatcgc gcagtatatc tccatgatgc ccgccgaaga ggacaacgag
     1201 cccctgcgcc aatggcgcat ccgctcctgg caggaccgca tcgcccccga aggatacgcc
     1261 ttccccggcg acccgcgcaa ctgggaacaa acccgctaca ccaccgccgt cctgtccccc
     1321 ctcgggcgca aactgctcgg cctcgatcgc tggtgatctc ccggccgctc ccacagacct
     1381 tacccttccg gggaacgccc agggaacgcc atttttggcc gaaggctttc ccattccctt
     1441 aacaattgcg gggatatctt tgtagttatt caaatcatcg catgaagcta tccccgctca
     1501 tatccgccgc cctcgccgcg acagcccttc tggcagcctc ctgcggctcc tcgcgccagg
     1561 cggcagccga ccctggtccc accacctggc aaaacgcccc catcaccgtc gacggatcgg
     1621 ataaggactg gacaaaacca ctcccctacg ccatctccgc cgagaagatc gcttattccg
     1681 tcaccaacga cgaccagaat ctctatatcc tcctctcgac aaaaaacccg caggaacaac
     1741 aaaagatcat ccagggtggg atgacgatct ggatcaacac aaaggcagat aaaagcgaat
     1801 ccgaggccgt cggcatcggg tacccccttg acagccgcaa cgaccacgac cggcaactca
     1861 tggaacaggc acaacccgat cgctataaga acaacaagcc cataaccctc gaagacaaaa
     1921 aggactacgc cctctatggt tttgacaaat catcggccgt cggcaactac acctacggcg
     1981 aagacaatcc ccagggcgta aagatgcgcc ttgactttaa caacgaaggc gaactcatct
     2041 acgaagcctc catcccgctg acaaccctgt atcccaacca taacccgtcc gcctcttatg
     2101 ccgccaacag cgtcgccatc ggcatcttca tcgaaggcct tccacccgac gcccatgttc
     2161 cccgcggaaa cggcggcggc ggccccgaga tcggtgtcgg cggaggcctc ggctttggct
     2221 ccttcggcag cggtggcggt atcggcatct cgatcggtac cggctccctc atcggcggcg
     2281 gcggcaggaa caaacaactg ttcagggaaa cccaaacctg gcaggtcgtg cagctggccc
     2341 gcggccactc ggcaaaactc gatcacccga cgaaccc
//
LOCUS       DATGFG010000721         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_8361, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000721 DATGFG010000000
VERSION     DATGFG010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8361"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="VKQ52_13450"
     CDS             <1..1697
                     /locus_tag="VKQ52_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88252.1"
                     /translation="ILMLTALLAFAGGITAQSILDRPVTLRFKQEPLGRVLETAGNAL
                     GITFSYNSAILNKDSLVTLTMESTPLRVVLDTLFKGRIEPHEDGRYIILLPAHKTDPP
                     PTEPKHYTINGIILDETTGKEVRDASIYDADQLIATLSKADGSFVVKLKGRRQPGSLT
                     VSKEFYTDTTIRLPPATEGRLTILLTPVFSPKPSTLSSPQPLSPDSISISWQADSAQI
                     RAIMNKDLVQVEMTGFGKFLLSSRLKIQTLNLKKLFIQRPVQVCLVPLLSTNGALNSQ
                     VTNKFSVNCVGGYSAGLEGTELGGVFNIDKRKMHGVQAAGVVNIAGDSVAGVQMAGVY
                     NQALDTMKGMQAAGAVNNARFVKGVQLSGAVNVASRVEGAQLSGGVNITRHLRGFQLG
                     IVNIADTSEGVGIGLLNIVKHGGIHELSLYTDEFSPLNIAFRSGNKALYTIFLAGLNP
                     GNDRRSYYTGFGLGHRFPLSPALALDVEASSAHVAPVAWKNYGDNTWLHRLNVDLHWQ
                     TGKWFALSAGPSMAIYTPQRDYTIQGHLYHPVGYSTFTFSGRNIGWIGWHAALNFL"
     gene            complement(1725..2507)
                     /locus_tag="VKQ52_13455"
     CDS             complement(1725..2507)
                     /locus_tag="VKQ52_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HLZ88253.1"
                     /translation="MDISGYLRRINYNGVVYRDLATLRELQYNHLFSIPFETLDIHNQ
                     VPILLQINFLYQKVIHDNRGGYCYELNVLFHNLLTLSGFNVSMIAGRLHHGNSKFGRE
                     FEHMALLVELHGRRWLVDVGYGDFSLTPLVIQPGEIQSDGRNFYQIIDPVIVDGRSYL
                     GVAKWNVSKQDFKIDYLFTLTPRALEDFHAMNEYHQTSPESHFRRSLICTLPTREGRI
                     TMINNKLIRTEGGKRQTKIIQDEYHRDEILEKYFHMDMVYLL"
     gene            complement(2623..3309)
                     /locus_tag="VKQ52_13460"
     CDS             complement(2623..3309)
                     /locus_tag="VKQ52_13460"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLZ88254.1"
                     /translation="MRMKFTFYGHATFSIEVNGKNLLFDPFFTGNPLIKNLDINTIKA
                     DYLFITHGHGDHTSDLVQVAKKTGAQCVAAAEIVNWLNKNGVGKTHPVNHGAPVAFDF
                     GKARAVNAIHSSSFSDGTYAGNPMGYVFTTPAGDFYVAGDTALTMDMQLIPLWAKLDF
                     AVMPIGGNYTMDPADAIHASDFVKCNKVIGVHYNTWPIIAIDTEKAKADFAAAGKELL
                     LPQPGETIEL"
     gene            complement(3366..>4031)
                     /locus_tag="VKQ52_13465"
     CDS             complement(3366..>4031)
                     /locus_tag="VKQ52_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88255.1"
                     /translation="AFDGQYARPVELTTAGIRRVVEEFKAAARRAMLAGFKVVEIHAA
                     HGYLIHEFLSPLSNQRHDEYGGSFENRIRLLVEIVDAVRSGWPPGYPLFVRISATDWA
                     EGGWSVEEAIRLAGVLKDRGVDVIDCSSGGLVPYQQIPAAPGYQVGFSQRIRNATGIL
                     TGAVGIITQAMQAESILVTGQADLIFLARQLLRDPYFPLHAAGELRQKDIHWPLQYER
                     ARL"
BASE COUNT          972 a         1284 c          939 g          836 t
ORIGIN      
        1 cgatactgat gctcaccgcc ctgctggcat tcgccggcgg cataacggcc cagtccatcc
       61 tcgatcgccc cgtcaccctc cggttcaaac aggagcccct gggccgggtg ctggaaaccg
      121 cgggcaatgc ccttggcatc accttctcct ataacagcgc catcctgaac aaagacagcc
      181 tcgttaccct gaccatggaa tcgaccccct tgcgcgtggt cctggacacc ctcttcaaag
      241 gccggatcga accccacgaa gacggccgct acatcatcct gctgccggcg cataaaacag
      301 acccgccgcc cacggagcca aaacactaca ccatcaacgg catcatcctc gacgaaacaa
      361 ccggtaaaga ggtccgcgac gccagcatct acgacgccga tcaactcatc gccaccctga
      421 gcaaagccga cggcagcttc gtcgtcaaac taaaaggcag gagacaaccc ggctccctta
      481 ccgtcagcaa ggaattctac accgatacca ccatccggct cccccccgct accgaagggc
      541 ggctcaccat cctgctgaca cccgtcttct cgccaaagcc aagcaccctg tcttcaccac
      601 agcccctgtc acccgattcg atcagcattt cctggcaggc cgattcggcg cagatcaggg
      661 ccatcatgaa taaagacctc gtccaggtgg agatgaccgg cttcggaaaa ttcctgcttt
      721 cctcccggct caagatccaa accctcaacc tgaaaaagtt attcatccag cgccccgtgc
      781 aggtatgcct cgtgccgctt ttaagcacca atggcgccct caactcccag gtcaccaata
      841 aattctcggt aaattgcgtc ggcggctact ccgccggcct cgaaggcacc gaactgggcg
      901 gtgtcttcaa catcgacaaa cgaaaaatgc acggcgtcca ggccgccggc gtcgtcaata
      961 tcgccgggga ctccgtcgcc ggtgtccaga tggccggggt ctataaccag gcactcgata
     1021 cgatgaaagg gatgcaggcc gccggcgccg tcaacaacgc gcgctttgtc aagggagtcc
     1081 agctctccgg cgccgtaaac gtcgccagcc gcgtcgaagg agcccaactc tccggaggtg
     1141 tcaacatcac ccgccacctc cggggatttc aactgggtat cgtcaacatc gccgacactt
     1201 cggaaggggt cggcatcggc ctgctcaaca tcgtaaaaca cggcggcatc cacgaactat
     1261 ccctctatac cgatgaattc tccccgctga acatcgcctt ccgatccggc aacaaagcgc
     1321 tatacaccat cttcctcgcc ggcctgaacc ccggcaacga ccggcgatcg tattacaccg
     1381 gcttcgggct cggccaccgc tttccgctgt cgcccgcact cgcactcgac gtcgaggcga
     1441 gctccgccca cgtcgcaccc gtggcatgga agaactatgg cgacaacacc tggctccatc
     1501 gcctgaatgt cgacctccac tggcagaccg gcaaatggtt cgctttatcg gccggcccct
     1561 ccatggccat ctataccccc cagcgggact acaccatcca ggggcatctc tatcaccccg
     1621 taggctattc cacttttacc ttctccggaa ggaacatcgg ctggatcggc tggcacgccg
     1681 ccctgaattt cctgtgatct tcgaatgtag atgatcgatg gtacctagag cagataaacc
     1741 atatccatat ggaaatattt ctccaatatc tcgtcgcggt gatactcgtc ctggatgatc
     1801 tttgtctgcc gcttgccacc ctcggtacgg atcagtttat tgttgatcat agtgatccgg
     1861 ccctccctgg taggcagggt gcagatgaga ctgcgacgga aatgcgattc gggtgaagtc
     1921 tggtgatact cgttcatcgc atggaagtcc tccagcgcac gcggtgtaag cgtaaaaagg
     1981 tagtctatct taaaatcctg cttggaaaca ttccatttgg cgacgccaag gtaggaacgg
     2041 ccgtcgacga tcaccggatc gatgatctgg tagaagttgc ggccatcgct ttggatctca
     2101 ccgggctgga tgaccagcgg cgtgagcgaa aaatccccat acccaacatc caccagccac
     2161 cgccggccat gcaactcgac aagcaacgcc atatgttcga actcacggcc gaatttactg
     2221 ttcccatgat gcaaccgccc ggcgatcatc gatacattaa aaccgctcaa ggtcaataaa
     2281 ttatgaaaaa gtacattcag ctcgtagcag taaccgcccc ggttgtcatg gatcaccttc
     2341 tgatataaaa aattgatttg caaaagaata ggcacctggt tatgaatatc cagggtctcg
     2401 aaagggatgg aaaaaagatg attgtactgt aactcccgca aagtagccag gtctctgtac
     2461 actactccat tgtaattgat ccttcttaaa taaccgctta tgtccatgct attagtttaa
     2521 tttatcaaat ccggtgtcta taggtcagtg cctggagtct attcctgcca gatatcttga
     2581 caattgcccg ccatcttatg ctgcaaaatc gtccaactta tcttacaatt cgatcgtttc
     2641 cccgggttgg ggtaagagca actccttacc ggccgccgcg aaatccgcct tcgccttttc
     2701 cgtatcgatc gcgatgatcg gccaggtgtt gtagtgaacg ccgatgactt tattacattt
     2761 cacaaaatcc gacgcatgga tcgcatccgc gggatccatc gtatagttcc ccccgatcgg
     2821 cataacggca aaatccagct tcgcccacaa ggggatcagt tgcatatcca tggtcagtgc
     2881 cgtgtccccg gcaacataaa aatcaccggc cggcgtcgta aaaacataac ccattggatt
     2941 accggcatac gttccatcgc tgaaagaaga agaatgaatg gcattcaccg cccgggcctt
     3001 cccaaaatca aacgcgacgg gcgctccatg attcaccggg tgagtcttcc cgacgccgtt
     3061 cttattgagc cagttcacga tctccgccgc ggccacacac tgcgcgcccg tcttcttcgc
     3121 cacctgcacc aggtcgctcg tatgatcccc gtgcccatgg gtgataaaca ggtagtccgc
     3181 cttgatggta tttatatcaa gattctttat caagggatta cccgtaaaaa atggatcaaa
     3241 taaaagattt ttaccattta cttcgatcga aaaggtagca tggccgtaaa aagtgaactt
     3301 cattctcatg tgattaaagg ttctgtgaaa acaaaccacc tgaacaaaag ttcaccccat
     3361 ccgttttaaa gtctcgcgcg ctcgtattgt aaaggccagt ggatgtcctt ctgccgaagt
     3421 tcgccggccg cgtgtaatgg aaaataagga tcccgcaata attgccgggc aaggaagata
     3481 agatcggcct gacccgttac caatatcgat tcggcctgca tcgcctgggt gataataccc
     3541 accgcacctg taaggattcc cgtagcgttc cggatccgtt gagagaaacc cacctggtat
     3601 cccggcgccg ccggtatctg ctggtaagga acaagcccgc ccgaagaaca atcgatcaca
     3661 tccacgcccc gatccttcaa cacacccgcc agccggatgg cctcctccac cgaccagccg
     3721 ccttcggccc aatccgtggc agagatccgg acaaacaaag gataacccgg aggccagccc
     3781 gaccgcacag catcgacgat ttccaccagc aaccgtatcc ggttctcaaa acttccgcca
     3841 tattcatcat gccgctggtt gctcagcgga gaaaggaact catggatcag gtaaccatgc
     3901 gcagcatgga tctccaccac cttgaacccg gccagcatgg cccgccgcgc cgccgccttg
     3961 aattcttcca ccacccgccg gatacccgcc gtcgtcaact ccacaggccg ggcatattgt
     4021 ccgtcaaagg c
//
LOCUS       DATGFG010000722         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_305322, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000722 DATGFG010000000
VERSION     DATGFG010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305322"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VKQ52_13470"
     CDS             complement(<1..644)
                     /locus_tag="VKQ52_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLZ88256.1"
                     /translation="MALYNALAIMTVLAALFAYLNHRFIRLPGTIGIMLISLLSSLVL
                     IVVGWVNAGVLSEMTRLVRSIDLYTILMKMMLSFLLFAGAIHLDSKGLRSQVLPVLTF
                     ATIGTVLSTFFVSVLLYGVCLVMGWPVEFIYCLLFGALISPTDPIAVIGILRQAQIPH
                     SLEMKISGESLFNDGVAVVLFISIYEIIEGGVAHLDAGHIAWLFLREAGGGILFG"
     gene            complement(720..2303)
                     /locus_tag="VKQ52_13475"
     CDS             complement(720..2303)
                     /locus_tag="VKQ52_13475"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ88257.1"
                     /translation="MLKRIIFCFSAQLAICNGYGQAQASAASTAFNQNQAAKPGYLQT
                     DSARAVALLRTPTYGNRSLLSRLFKKRDTAGIVAEPASAPFAFGDFSWLNGNSRKTSP
                     PLIDSKYFTSDITLDLNYTHSYNHPIDNTVVGSTALARNNELQLSFLGFGGDIHVDNV
                     RGRIMMQFGTRSTVVPRNDGSSYKGQYDLQTIYRYISEAYAGYHWDVWHGINLDAGIF
                     MSYIGLFSYNNFENWGYQPSYTSDNTPWFFNGLRLQTFPTDKLKVELWLINGWQSYGM
                     FNHSPGAGFSIYYRPKETVDYVSNFYYGKDDQGAPGRYRLHSDDSYELRYFNRKHGFV
                     TKAAFSVTGDFGFENGNGVSPFGKGVKGTASYLPAQNFISGMIYNRLWFGEKQKFAWT
                     IGGGYMHNPGQYLVLAPTGYADTLYQQQTGPGSTFDAWDCSTSLDYMPSQNLTLKFEY
                     VHRKVNSMDAAPGTTPLNGYFAGPGGVTSPTGFANTGGYTYASNGASIAPPLSSWGGW
                     GPDLRNSESRIILALIVRF"
BASE COUNT          641 a          771 c          556 g          476 t
ORIGIN      
        1 ccaaacagga tcccaccccc cgcctcccgc aggaaaagcc aggcgatatg gccggcatcc
       61 aggtgcgcca ccccgccttc gatgatctca tagatgctga taaaaagaac caccgccacc
      121 ccgtcgttga acagcgactc cccactgatc ttcatctcca gcgaatgcgg gatctgcgcc
      181 tgccggagga tcccgatgac ggcgatagga tccgtgggcg agatcagggc gccaaaaagc
      241 aggcaataaa taaattcaac gggccacccc atcaccagac aaaccccata aagcagaacg
      301 ctcacaaaaa aagtagacag caccgtcccg atggtcgcaa aggtcagcac cggcagcacc
      361 tgcgatcgca gccccttgct gtccaggtgg atcgcccccg caaaaagcag gaagctcagc
      421 atcatcttca tcaatatcgt atacagatcg atgctcctga ccagccgcgt catctcgctg
      481 agcacccccg cattgaccca gccgaccacg atgagcacaa gcgaggaaag caacgagatc
      541 agcataatgc cgatcgtccc cggcaaccgg atgaaacgat gattcaggta ggcgaacaac
      601 gccgccagca ccgtcattat cgctaaggca ttataaaggg ccatcctgga attatttatc
      661 ccaagatagc ccttccaatg caatcgccct aaaaccgaat accccggacc cgggataccc
      721 taaaaccgta cgatcagcgc caggatgatc ctcgactccg agttccgcag gtccggcccc
      781 caaccacccc agctgctcag cggcggcgca atcgaagccc cgttcgacgc atacgtatag
      841 ccacccgtat tggcaaaccc cgtcggcgaa gtaacccctc ccggaccggc aaaatagcca
      901 ttcaacggcg tcgtgcccgg cgccgcgtcc atcgaattca ccttgcggtg cacatactcg
      961 aatttcaacg tcaggttctg gctgggcata taatccaggg aagtcgaaca atcccaggca
     1021 tcgaaggtcg aacccggacc cgtctgctgc tggtataacg tatccgcata gccggtagga
     1081 gccagaacga ggtactggcc ggggttgtgc atatacccgc caccaatggt ccaggcaaac
     1141 ttctgcttct ccccaaacca aagccggtta tagatcatcc cgctgatgaa attctgcgcc
     1201 ggcagataac tggctgtccc cttcaccccc ttgccgaatg ggctcacccc gttcccattc
     1261 tcaaaaccga aatcgcccgt caccgaaaaa gctgccttcg tcacaaaccc atgtttgcga
     1321 ttgaagtacc gcagttcata gctgtcatcg ctatgaaggc gataacggcc cggcgcgccc
     1381 tggtcatcct tcccatagta aaagttcgaa acgtaatcca ccgtttcctt cggccggtaa
     1441 tagatcgaaa aacccgcacc cggcgaatgg ttgaacatcc catagctctg ccagccgttg
     1501 atcagccata actccacctt caacttgtcc gtcggaaagg tctgcaaccg caatccatta
     1561 aagaaccagg gcgtattgtc cgaagtatag gaaggctgat aaccccagtt ctcaaaattg
     1621 ttgtaagaga acaacccgat ataggacatg aagatccccg catccaggtt gatcccatgc
     1681 cacacatccc agtgataacc cgcataggcc tcgctgatat accggtaaat ggtctgcaga
     1741 tcatactgcc ccttgtacga actcccgtca ttccgcggga ccaccgtact ccgcgtacca
     1801 aactgcatca tgatcctgcc ccgcacattg tcgacatgaa tatccccccc aaaaccaagg
     1861 aacgaaagct gaagctcgtt gttcctcgcc aaagccgtac tccccaccac cgtattgtcg
     1921 atcggatggt tataggaatg cgtataattc aggtccagcg taatatcact ggtaaaatac
     1981 ttgctgtcga tcagcggcgg gctcgttttc cggctgttcc cgttcaacca ggaaaaatcc
     2041 ccaaaggcaa acggtgcact ggcgggctcc gccacgatgc cggcagtatc ccgctttttg
     2101 aaaagccggg aaagtaaact cctgttccca taggttggag tccgaagcag cgcaaccgcc
     2161 cgcgccgaat cggtttgcag ataacctggt tttgccgcct gattttgatt aaaagccgta
     2221 ctcgccgcag aagcctgcgc ctggccatac ccattgcaaa tggctagttg cgcactaaaa
     2281 caaaaaatga ttcttttaag catacgctcg ttttggtttt aaagtgaata ataacaggca
     2341 agacaaccgc agacaaatcc catcttcttc caaacctata ccagagcgcc tggtccccgg
     2401 ctcctttact ctaagacgca ggactatcag gggtttgggt taaa
//
LOCUS       DATGFG010000723         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1520625, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000723 DATGFG010000000
VERSION     DATGFG010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520625"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VKQ52_13480"
     CDS             complement(<1..589)
                     /locus_tag="VKQ52_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HLZ88258.1"
                     /translation="MPAISGDIQLEEDAKRIVRIAQAIARENMNAVLSPAHLLKALLH
                     KDAGLQGRLKQLDQDIYYLEEWADVRIESVPKSPTPKESPAGDALLEEVFSEADNIRL
                     KLSKDTISPLHLLAAISTPGVGFTYDQLKTYPLKRDQLLQQATESEDLRATIGAANNG
                     AGAAKQAANTGNHALLKYCVDKNLRAREGKSDTVIG"
     gene            complement(604..1644)
                     /locus_tag="VKQ52_13485"
     CDS             complement(604..1644)
                     /locus_tag="VKQ52_13485"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HLZ88259.1"
                     /translation="MNRRSSSLFRVDEQAWLTMGAVCLVALIVLAFRFGTSHPCYPIR
                     ITSSAATYAVGSPVSFKAETRLGKVYEWNFGDGATSKEDDPITLHTYSRAGKYTVVIT
                     VDGQCSEMQIVSIGEAPITVNNNLPPVIIGPDTAYVGKPVNFEDGTAGSTSWAWHFEG
                     VAMVDAYTRKASHTYLTGGPKKIILEVNHRPELTNSRLIFVVDKQAAAPRVQKSKGDD
                     NGGRPKIISVPEKPVTPPLTLPSDQPKKEEEKPKAPPVTDDKLQSMLLQVVDGTMVQD
                     DFGAYICNNMNIPVMYNGAKTTFSAMCADLKNQKRKKIKKISVTTTRVDATNCIVSMD
                     VTVEKKKSFLGL"
     gene            1824..2426
                     /locus_tag="VKQ52_13490"
     CDS             1824..2426
                     /locus_tag="VKQ52_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HLZ88260.1"
                     /translation="MNRVCAFLIPCFGAFLLSCSSSKKTVQPASGDIIITTKEGHSTG
                     EGAHKEYDSIQVKYAGYLHTTPEQVQNIRLYRFIDKWLYTPYKWGGTDEKGIDCSSFM
                     QKLLSEVYRVEIPRTSVQQFFDGWIERYGSAQYLSEGDLVFFQTIGENAVSHVGLYLG
                     NHMFVNASSSKGVSIASLEDPYWKKRYVAAGRVKRSPLSQ"
BASE COUNT          483 a          625 c          767 g          640 t
ORIGIN      
        1 ggccgatgac ggtgtcgctt ttcccttcgc gggcccggag attcttgtcc acacaatatt
       61 ttaacagggc gtgattgccc gtgttggccg cttgtttggc ggcgcccgcg ccgttgtttg
      121 ccgcgccgat ggtggcgcgg agatcctcgg attcggtcgc ctgctggagc agctggtcgc
      181 gtttcagcgg ataggtcttc agctgatcgt aggtgaaccc gacacccgga gtgctgatgg
      241 cggcgaggag gtggaggggg gagatggtgt cctttgacag tttgaggcgg atattgtccg
      301 cctcgctgaa tacctcttcc aacagggcat cgccggcggg agactctttt ggagtcgggg
      361 atttgggaac ggactcgatg cggacatcgg cccattcttc caggtaatag atgtcctggt
      421 ccagctgttt taaccgtccc tgcaggccgg catccttatg gaggagggcc tttagcaggt
      481 gggccgggga aaggacggcg ttcatgttct cccgggcaat ggcctgggcg atgcgcacga
      541 tgcgtttggc gtcttcctcc aattgtatat ctccgctgat ggcaggcatt cgagatgcat
      601 tttttacaat cctaaaaagc ttttcttctt ttcgacggta acgtccatgg agacgataca
      661 gttggtggcg tccaccctgg tggtggtgac cgagatcttc tttatcttct tccgcttctg
      721 gttcttcagg tcggcgcaca tggcgctgaa ggtcgtcttc gcgccgttgt acatcaccgg
      781 aatgttcata ttattacaga tataggcgcc gaaatcgtcc tggaccatgg ttccgtcgac
      841 gacctggagg agcatactct gcagtttgtc gtcggtgacg gggggcgcct ttggcttttc
      901 ctcctctttc tttggctggt cggacggaag ggtcaggggc ggcgtgacgg gtttttcggg
      961 gacggagatg atctttggcc ggccgccgtt atcgtcgcct tttgactttt gtacgcgggg
     1021 agccgctgcc tgtttgtcga ccacgaagat gaggcgggag ttggtcagct cgggccggtg
     1081 attgacctcg aggatgatct ttttcggtcc gcccgtgagg taggtgtggg aagctttgcg
     1141 ggtgtaggcg tccaccatgg cgactccttc gaaatgccat gcccaggagg tggagccggc
     1201 ggtgccgtct tcgaagttga cgggtttgcc gacataggcc gtatccggac cgatgatcac
     1261 gggaggcagg ttattgttta cggtgatggg tgcttcgccg atgctgacga tctgcatttc
     1321 actgcactgg ccatctacgg tgatgactac ggtatatttt cccgcccggc tgtaggtgtg
     1381 cagggtgatg gggtcatctt ccttcgacgt ggcgccgtcg ccaaaattcc attcatagac
     1441 cttccccagg cgggtttcgg ccttgaaact caccgggctg ccgacggcat aggtggccgc
     1501 ggacgaggtg atccggatcg gatagcaggg atgagaagtg ccaaaacgaa aggcgagcac
     1561 tatcaaggcg accaggcata ctgcccccat ggttagccag gcctgttcat ccactctgaa
     1621 taaggacgaa gatctcctgt tcatagttta ctatgcttaa gactgtttaa ctactgcgac
     1681 gatgttgtct aggatgtgga tcggaggata ttaagacggg cacttatcgg atgtcagggg
     1741 atgccaaatt aaacaataaa gcccgaacct gcaacgtgga aaaccacctt tttttagcga
     1801 atttttctac cttcatagta tctatgaaca gagtttgcgc ctttctgatc ccctgttttg
     1861 gagcttttct tctctcctgc agcagcagca agaagacggt tcaaccggcg agtggtgata
     1921 ttatcattac cacgaaggaa gggcattcca ccggagaagg ggcccataag gaatacgaca
     1981 gtatccaggt gaaatacgcg ggctatcttc acaccacacc cgaacaggtg cagaatatcc
     2041 ggctttaccg gttcatcgac aaatggctgt atacgcctta caagtggggc gggacggatg
     2101 agaaggggat cgattgttct tcctttatgc aaaagctgct gtccgaggtt tatcgtgtag
     2161 agataccccg gacatcggtg cagcagttct tcgacgggtg gatcgaacgg tatggatcag
     2221 cccagtacct gtcggaaggt gatctcgtct tttttcagac cattggagag aatgcggtgt
     2281 cgcatgtcgg tctctacctg ggtaatcata tgtttgtcaa cgcctcctcc tcgaagggcg
     2341 tgagcattgc cagtctcgag gacccttact ggaagaagcg gtatgtggcg gcgggcaggg
     2401 tgaaacggtc gccgctttcg cagtaagacg ttagttcctg atgatccggc ggccctgggc
     2461 gtcggcgggt cccatcagct catccatctt tttcatatag tcagcgcggc tgatc
//
LOCUS       DATGFG010000724         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_489059, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000724 DATGFG010000000
VERSION     DATGFG010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489059"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1140
                     /locus_tag="VKQ52_13495"
     CDS             94..1140
                     /locus_tag="VKQ52_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HLZ88261.1"
                     /translation="MAIAKLEYIWLDGYHPIQSLRSKTKIERKFSGKLEDCPMWCFDG
                     SSTEQAPGGSSDCLLKPVFLAKDPQRRDGYLVMCEVLSPDGKPHPTNGRALIEDDDND
                     FWFGFEQEYFLWNPETDKPLGFPEGGFPRPQGPYYCSVGARNAFGRDMVEEHLDACLD
                     AGLNVEGINAEVATGQWEFQVFAKGAKEAGDQVWIARYLLERIGEKYGIAINWHCKPL
                     GNLDWNGSGMHANFSNSLLRTCGSSEIYVKVCEAFRPFVSEHIEVYGPDNHLRLTGKH
                     ETAAINEFKFGVSDRGASIRIPIATVERGWKGWLEDRRPNSAADPYKVAARIVKTVKS
                     AEAQLTKLVEANVA"
     gene            complement(1195..1485)
                     /locus_tag="VKQ52_13500"
     CDS             complement(1195..1485)
                     /locus_tag="VKQ52_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HLZ88262.1"
                     /translation="MFNDLDPLLHSQLRLAIVSLLMNYEEANFIFIRERTKATDGNLS
                     VQLSKLKTAGYIEVKRRIRDNYSETVCKITPAGIKAFEQYVKDIKLYLKVRK"
     gene            complement(1478..2095)
                     /locus_tag="VKQ52_13505"
     CDS             complement(1478..2095)
                     /locus_tag="VKQ52_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88263.1"
                     /translation="MEDKQIQSAEESIAIIEKMIEQAKKSVADNSFLFLSWGWLLFGA
                     LITQFMLKVIFRYPYHYYAWALMYLVLAFLLFYLKRKVAKRMTTTFIDRAIQYLLICL
                     VISSLLLTLVFMRIGWENCYTFYMVIMAAGCFLAGKLLQYRPLVIASVLCAVMAMLST
                     YISFDGKILLCGFAVLVSYIIPGYLLRSAYRRGALNYGYKPDPNV"
     gene            2175..2573
                     /locus_tag="VKQ52_13510"
     CDS             2175..2573
                     /locus_tag="VKQ52_13510"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HLZ88264.1"
                     /translation="MLFLFFLLLFGSRPLQPATLHVVVQNVQTGKGSINVAVFGNRSD
                     FLKRPVAAQIRKADTGTLEFSFAIPRGEYAVAVYQDLNENQQLDAGIFHIPKEPYGFS
                     NHYRPRMSAPKYEDCRIAVAGDTRCIIQLK"
     gene            2579..>2875
                     /locus_tag="VKQ52_13515"
     CDS             2579..>2875
                     /locus_tag="VKQ52_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88265.1"
                     /translation="MKKLLLLSNIALSMIGAGQVWLVQLSSYPLWAHVGPAEFREYHI
                     AWWHSIWGPIFVPAGMSILCTIALLRYPTLARRALWVGLLLLILTYGLTVVWWAP"
BASE COUNT          674 a          716 c          777 g          708 t
ORIGIN      
        1 atttatcata ttgtatcttt ttactattcg tcataacccc aactggttgt atcttcgtta
       61 tggttgagaa ttgacaatta aataatagat acaatggcta tcgcgaaatt agagtatatc
      121 tggctcgacg ggtaccatcc cattcaaagc ttacgcagca agacaaagat cgaacgtaaa
      181 ttttccggca aactggaaga ctgccccatg tggtgtttcg acggatcatc caccgagcaa
      241 gctccgggcg gttcatcgga ttgcctgctg aaacctgtct tcctggcgaa ggatccccag
      301 cggcgcgatg gttacctcgt gatgtgcgaa gtattgagtc ctgatggcaa gcctcatccg
      361 accaatggtc gcgccctcat cgaggatgat gacaacgatt tctggtttgg cttcgagcag
      421 gaatatttct tatggaatcc cgaaacggat aagcccctgg gttttccgga aggcggcttc
      481 ccaaggccgc agggtcctta ctattgctcg gtgggcgcac gcaacgcctt tggccgggac
      541 atggtggaag aacacctgga tgcctgcctc gatgccggcc tcaacgtcga gggtatcaac
      601 gcggaagtag ctaccggtca atgggagttc caggtgttcg cgaagggcgc caaggaagcc
      661 ggggaccagg tttggatcgc gcgttattta ctggaaagaa taggtgaaaa atatggtatc
      721 gccatcaatt ggcattgcaa gcccctgggc aacctggact ggaacggcag cggcatgcac
      781 gccaatttct ccaacagcct gctgagaaca tgcgggagca gcgaaatata tgtgaaggtg
      841 tgcgaagcct ttcgtccctt tgtgtcggaa catattgaag tatatggccc cgataaccac
      901 cttcgtctca ccggcaagca tgagacggcg gcgatcaacg aattcaaatt cggcgtatcg
      961 gaccgtggcg catccatccg gatcccgatt gccacagtgg aaagaggctg gaagggctgg
     1021 ttggaagacc gccggcccaa cagcgctgcc gacccgtata aagttgcggc ccgcatcgtc
     1081 aagacagtga aatcggctga ggcacaactg acgaagctgg tcgaggcgaa tgtggcgtaa
     1141 ggttgccggt tgaatgccgg tcggttgcgg gtcgccggtt gtcgcttacc aggattattt
     1201 acggaccttc aggtagagtt ttatgtcttt gacgtattgc tcgaacgcct ttattccggc
     1261 gggtgttatt ttacagaccg tctccgaata attgtccctg atccttcgct tcacctctat
     1321 atagccggcc gtcttcagct ttgacaattg aacgctgagg ttgccgtcgg tagcttttgt
     1381 tctttcgcgg ataaaaatga agttagcttc ttcgtaattc atcaacagcg atacgatggc
     1441 gagcctcaac tgggaatgca gcaggggatc aagatcgtta aacatttgga tccggtttat
     1501 acccgtaatt cagtgctccc cgcctgtagg ccgaccgcag caggtagccg gggatgatat
     1561 agctgaccag cacggcaaat ccgcagagca ggatcttgcc atcgaaggag atataggtac
     1621 tgagcatggc catcactgca catagaacgg acgcaatgac cagcgggcga tactgcagca
     1681 gtttgccggc aagaaagcag ccggccgcca tgatcaccat gtaaaacgta taacaattct
     1741 cccaccctat ccgcataaag acgagggtca gcaggaggga agagatgacg agacagatca
     1801 gcaggtattg aatggcgcga tcgataaagg tggttgtcat gcgtttcgcc acttttcttt
     1861 tcaggtagaa caacaggaaa gccagaacga ggtacatgag cgcccaggca tagtaatgat
     1921 agggatacct gaatatcact ttgagcatga attgcgtgat cagggctccg aacaggagcc
     1981 agccccagga taagaacaga aagctgttgt ccgccacact ctttttcgcc tgttcgatca
     2041 tcttttcgat gatcgcaata ctttcctcag cagactgtat ttgtttgtct tccatggtac
     2101 atattttgca ttgtaaatat agttacttta ttttataaag tgctttatat ttatagtaca
     2161 aaaaccggtt gccaatgctt ttcctttttt ttcttttatt gttcggtagt cgtcctttgc
     2221 agcccgccac cctgcatgtc gtcgtacaaa atgtccagac gggcaaggga agtatcaatg
     2281 tcgcggtgtt cgggaacagg agcgatttcc tgaagcggcc ggtggcggct cagatccgca
     2341 aggccgatac cgggacgctg gaattctcct ttgccatccc gcggggcgag tatgcggtcg
     2401 ctgtctacca ggacctgaat gagaatcagc agctcgatgc ggggatcttt cacattccga
     2461 aggagccgta tggattcagc aaccattacc ggccgcggat gtccgcgccc aaatatgagg
     2521 actgccgcat tgcggtggcc ggggataccc gctgtatcat tcaattaaaa taaacctcat
     2581 gaaaaaatta ttgctgctgt cgaatattgc cctgagcatg atcggggcag gacaggtctg
     2641 gctggtgcag ctttcctcct atccgctttg ggcgcatgtc ggtccggcag agtttcgcga
     2701 gtatcatatc gcctggtggc atagcatttg ggggcccata ttcgtccctg ccggaatgtc
     2761 gatcctttgt accatcgccc tgttgcgata ccctaccctt gcccgcaggg cgctgtgggt
     2821 agggcttctc ctgctgatac tgacctatgg gctcaccgtt gtctggtggg cgcct
//
LOCUS       DATGFG010000725         8110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1500229, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000725 DATGFG010000000
VERSION     DATGFG010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500229"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VKQ52_13520"
     CDS             <1..515
                     /locus_tag="VKQ52_13520"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HLZ88266.1"
                     /translation="GLERGMLHRYFREFWDLPREIDDTAAGYLILLIPYAHRIAGHLL
                     LMQQCLELLLGEDTEVRIVPPQPTLIDRQMEKGLGEQSLGDDTVCGSSFMEGYPAYEY
                     IIGPLDQDKVKEYLPGGHRYLIIETFNRFFVPAHASVNTQIEIDRQTAEMKLEPGKEP
                     LLGYSSLLMG"
     gene            526..1401
                     /locus_tag="VKQ52_13525"
     CDS             526..1401
                     /locus_tag="VKQ52_13525"
                     /inference="COORDINATES: protein motif:HMM:NF036941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssN family type VI secretion system protein"
                     /protein_id="HLZ88267.1"
                     /translation="MQAERWLYPEMGRWMMLLGVGAIGLSALMGLLIRRIRGGFKAFS
                     RRTIFYTLVYAACFALIGLCIGLPLVHQYMYYFIFFQVLYTMAGIIHLFTMRYWLKWT
                     GPGAFWPELLHLLIIMLLGAVCMIMTYRILNREGAEIAMSTAALFFLVPYFVYQTYER
                     AVAIPPKIFRQWYYPLHSSEPELDETKLKNLLVISFEFQKHPTDRYFTNFRAKAPVDM
                     EFGELFYYFINDYNERHNDAAIRYSSNEGDTHGWIFYRKPKWYHLSTRYIDTEKTVFI
                     NNIRENDVIICNRVS"
     gene            1411..2592
                     /locus_tag="VKQ52_13530"
     CDS             1411..2592
                     /locus_tag="VKQ52_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88268.1"
                     /translation="MQQQSATYLPVNWTDGMKINKDHFRAEQQAYNWQQAQHSGALLN
                     GHNYGLFHSRQSRGSDRQIVLTTDNQQQVTIRLLTCHAITPGGHLVCFNEGSTAREDH
                     FMAGIPGLQVPYAQLIAADTEYYVTLAVNPYDRVPVGQPNMTETQMRPPFTAPAYSLQ
                     LIDHRDMPVGGPGSFYLPLGRVRVSEGKVLPDETYIPPCTTVAAHSALTDAHAGLEHF
                     FSSMELYTLRIQQKIVQKRQQNNLASVIMEICDKMNVYMSGEYQRVLLEYQQRPPVCL
                     LTSVAGLARLMKNSIDIYTGAIKDELLNYFAEWCSITQGEWESASTAICNFRYDHLNI
                     REALQQVEGFTQLTLRLFMSLASLEYIGKKREAGIFVKEHLVVPGEEMQVARRKSFLA
                     D"
     gene            2682..3209
                     /gene="tssO"
                     /locus_tag="VKQ52_13535"
     CDS             2682..3209
                     /gene="tssO"
                     /locus_tag="VKQ52_13535"
                     /inference="COORDINATES: protein motif:HMM:NF036668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system TssO"
                     /protein_id="HLZ88269.1"
                     /translation="MAHIINAKERNTAFWKFLGFFLLSTLLVVSAVYFDTRIPAKDNA
                     IMREQISSYKTQALAQDKFVRSMDEAKALIDSLNRPGSNNVYLNQQIAARIRELAQLQ
                     YKDSSMYSRLNKNVLDVFLRYQEATNKVVSIGDMPRQLEDYKAKYEQAQRDLDNTRRD
                     LDVLRRSNASTQGSF"
     gene            3241..4272
                     /locus_tag="VKQ52_13540"
     CDS             3241..4272
                     /locus_tag="VKQ52_13540"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HLZ88270.1"
                     /translation="MNNRQIAYKRVDDKAIITMIVLAVLSLLVMAFRYANHTVCPSFN
                     LAVRGNHYTTGEVIRFETNTRDARILHWSFGDNQSNDTKLPSAVHAYDEPGEYTVSLT
                     VNGECTEYARVSIGPAPVVENPRLVPRFVYPQSAEVGKPVQFSDTTVGAQQWQWRFGE
                     TATVDATDKSPRYTYTTPGLKTVSVVINNDERQMGICKIYVNPPPPPKRKDHGGNDRP
                     IIVVHDKPGQAPAGEQPSTAKTEPAPVKAPDITGPEFETQLRAVANKFKNAESFSAYF
                     CGNLNTQVSLNGQMIPFTELCNKFGSLKNEKKIKKLTVQLVKNEQTNCIISMFVTLKE
                     REGLFNKIF"
     gene            4275..6776
                     /locus_tag="VKQ52_13545"
     CDS             4275..6776
                     /locus_tag="VKQ52_13545"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HLZ88271.1"
                     /translation="MNDLTMDIQPSPALRQALHIAQAVAREHMHGDFGAPHLLKALLH
                     RDLGVEDQLWKMDQDVYYIAEWADVRLESCRKTPAPKDAPAGDEQVAAILDEADNIRL
                     QLNEEEISPMHALIALSTPGVGFSFEQLKTFPLQRDALLQWQQDHDMLRNLSQPASPS
                     GQGQQEQRQRGTQSLLKFCREKTLQAADGTMDPIIGREQEIRMMAEIISRRSKPNVLL
                     IGEPGVGKSALVDGFASAIHNGQVPEFLRQARLFELDNGTLVAGASYKGEVEERIKSI
                     LQEIKTFDKAILFIDEIHTLLDRQGAAAGAANLLKPELARGTITVIGATTLEEYRKHI
                     EKDEAFARRFELLTVSEPDATTTFRMIRTVLPRYEVHHRVRADDETLKATIRLARRYI
                     KGRKLPDAAIDLLDRSMAALRLAIDTGMASKEKFLLEFGRMKNTALCAAPEDLQWHYG
                     QLKNALGPLIWTEAVSGTASGAANEAASEADLLSEISCVYQRLADILPVERTQLNQQD
                     VASIVADKTGIPIGKLQSQEQDRLLNMPDALRQRIIGQDHAVRTISEAILESRSGLSK
                     PGQPIGSFFFLGPTGTGKTELAKALAEFMFQDEKFMIRFDMSEFKEEHAAALLYGAPP
                     GYVGYEEGGLLVNKIRQQPYAVVLFDEIEKAHASVFDIFLQIMDEGKLHDKLGREGDF
                     SNAIVIFTSNIASRYVTEAFAKGSTPTHAELLEKMAAHFRPEFLGRLTEIVPFGPVDP
                     QMVEHIFSIQVAALHHLLSAQGIELRITPAAAKHVAQSGFSPDYGARPLAGVIRSHLR
                     RPLSRMIVSGELQHGSKITMDLGPEQRLIWLPGQT"
     gene            6811..7263
                     /locus_tag="VKQ52_13550"
     CDS             6811..7263
                     /locus_tag="VKQ52_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88272.1"
                     /translation="MAIEPYGIGGTEVRTDAFEAFADIPQNRVLLAEKLTKDPPVKPD
                     IVNGLTDIISVFKHFDPKVDIEFESEEGTIRGETLHFHGLEDFGAKGITAQSPYLKEL
                     NMKKQQYHKIIRQLKTNKLLKQAIAGKETKENLILALQSLIRELETAP"
     gene            7317..>8110
                     /locus_tag="VKQ52_13555"
     CDS             7317..>8110
                     /locus_tag="VKQ52_13555"
                     /inference="COORDINATES: protein motif:HMM:NF036663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88273.1"
                     /translation="MAVEQTKAVNSENPVYETLTKPAESLDGQLVGLAKFGGFDLLES
                     AVDAVQNLNPSRKARKKIFLEEQSKKEERSELKKTLQWWVDILQSSDDITDMVKNCNE
                     KAAVAEKTLNRNLKHAVSETAELERSYRSVALFFKNTESLKVKNVSIINAEPDQLKDL
                     DNTRFIDTIRVELINNYDRLDLRNNYSLLILPGYLGSNKVVEKWAKIAYENKVMLITD
                     FEHLDSPDDVMELFEAANLTGGDPWRSNVIMTCNWLVGRGKIDEIE"
BASE COUNT         2166 a         2124 c         2117 g         1703 t
ORIGIN      
        1 tggggcttga acggggcatg ctgcaccggt atttcaggga attctgggac ctgccccggg
       61 agatcgacga taccgcggcc ggctacctta ttttgctgat accctatgca caccgcatcg
      121 ccggtcatct tctcctcatg cagcaatgcc tggagctcct gctgggagaa gatacggagg
      181 tccgcattgt gcccccacag ccgaccctga ttgaccggca aatggaaaag ggcctgggtg
      241 aacagtccct gggagacgac acagtttgcg gcagctcctt catggaagga tacccggcct
      301 atgaatacat tatcggaccg ctggatcagg acaaagtaaa ggaatacctg ccaggcgggc
      361 atcgctacct tatcattgaa acttttaacc ggttttttgt ccctgcccat gcttcggtga
      421 atacgcagat agaaattgac aggcagaccg cggaaatgaa actggagccg gggaaggaac
      481 ccctgctcgg atattcatcc ctgctcatgg gctaaaaaat atcatatgca ggcagaaaga
      541 tggctttacc ctgaaatggg tcgttggatg atgttactgg gagttggggc tatcggcctc
      601 tccgccctca tgggccttct gatccgcagg atcaggggcg gattcaaggc attcagcagg
      661 cgcactattt tttataccct tgtttatgcc gcttgctttg ccctgatagg gctttgtatc
      721 gggctccccc tggttcatca atacatgtac tattttatat tcttccaggt gttgtatacc
      781 atggcaggga ttattcacct gttcaccatg cgctattggc tgaaatggac cggccccgga
      841 gctttctggc ccgaattgct gcacctgctc atcatcatgc tgctcggcgc ggtatgtatg
      901 atcatgacct atcgtatcct caatagggaa ggggcggaaa tcgccatgtc caccgctgca
      961 ctgttcttcc tggtgcctta ttttgtctat cagacctatg aaagggcagt tgccattcct
     1021 cccaagatat tcaggcagtg gtactatcca ctgcatagca gcgagccgga attggatgag
     1081 accaaactaa agaacctgct cgtcatcagc tttgagtttc aaaagcaccc cacggatcgc
     1141 tactttacca acttccgggc caaggcgccg gtagacatgg aattcggaga gctcttttat
     1201 tatttcatca acgactataa cgagcggcac aatgatgcag ccatccgcta ttccagcaac
     1261 gaaggagata cacacggctg gatcttttac aggaaaccga agtggtacca cctgtccacc
     1321 cgttatatcg acacggaaaa aacagtattc atcaacaata tcagggaaaa tgacgtcatc
     1381 atttgcaacc gggtatctta atattttata atgcagcaac aatccgcaac atatctgcct
     1441 gtcaactgga ccgatgggat gaagatcaat aaagatcatt tccgcgccga acagcaagca
     1501 tacaactggc agcaggcgca gcattccggc gccctgctga atgggcacaa ttatggattg
     1561 ttccactccc gtcaatcgcg tggcagcgac aggcagatcg tactaaccac ggacaatcag
     1621 cagcaggtga ccatcaggtt gctgacctgc cacgccatta ctcccggcgg ccacctcgtt
     1681 tgttttaatg aaggatcgac tgcgcgggaa gaccatttta tggcaggtat ccccggcctg
     1741 caggtgccat atgcgcaact gattgccgcc gataccgaat attatgtgac cctggcggtg
     1801 aacccctatg acagggtgcc ggttggccag cctaatatga cggagacgca gatgcgcccg
     1861 cccttcaccg cacccgctta tagcctgcaa ctgatcgatc accgggatat gcccgtggga
     1921 gggcccggta gcttttatct gccattgggc agggttcgcg tcagtgaggg aaaggtcctg
     1981 cctgatgaga catatatccc tccctgcacc accgtggcag cacacagcgc attgacggat
     2041 gcacatgcag gactggagca cttcttcagc agcatggaat tatataccct gcgcatacaa
     2101 cagaagatcg tgcagaaaag acagcagaac aaccttgctt ctgtcatcat ggaaatatgc
     2161 gacaagatga atgtgtacat gagcggggaa taccagcgtg tattgctgga ataccaacaa
     2221 cggccccctg tctgcctgct caccagcgtt gccggcctgg caaggttgat gaagaacagt
     2281 atcgatatat atacgggagc catcaaagac gagctactga attactttgc cgaatggtgc
     2341 agcatcacac agggagaatg ggagtcggca tccacagcca tctgtaattt caggtacgac
     2401 cacctgaaca tccgggaagc gctgcagcag gtggagggtt ttacgcaact gacgctccgg
     2461 ttgttcatga gccttgcttc gttggagtat atcggcaaga agagggaagc gggaatattc
     2521 gtaaaggagc accttgtagt cccgggtgag gaaatgcagg ttgccaggcg caaaagcttt
     2581 ctggcggact aggttgtttt gaatgttgga tgttaaatgt taaatgtcga atcagggatc
     2641 gcttgcattc aaaatttaag acttaaaatt taaaataata aatggcacac attattaatg
     2701 caaaagagcg caataccgcc ttttggaaat ttctgggctt cttcctgcta tccaccctgc
     2761 tcgtggtcag cgccgtctat tttgacaccc gtatcccggc taaggacaat gccattatgc
     2821 gggaacagat cagcagctac aagacacaag ccctggcaca ggacaaattc gtcaggagca
     2881 tggatgaggc caaggccctc atagactccc tcaaccgtcc cggcagcaac aacgtctacc
     2941 tgaatcagca gatcgcagcc aggatcaggg agctggctca actgcaatac aaagacagca
     3001 gcatgtacag ccggttgaac aagaacgtcc tggatgtatt cctgcgttac caggaggcaa
     3061 ccaataaggt cgtcagcata ggggatatgc cccgccagct ggaagactat aaagcgaaat
     3121 atgaacaggc ccaacgcgac ctcgacaaca ctcgtcgcga cctggacgtc ctgcggcgca
     3181 gcaatgccag cacgcagggt tctttttgaa caatggccgg tggtaaataa acaacagcat
     3241 atgaacaaca ggcagattgc atacaagcgg gtagacgaca aggccatcat taccatgatc
     3301 gtactggcgg tattgagctt gctggtcatg gcatttcgct atgccaatca taccgtttgc
     3361 ccttccttca accttgctgt ccggggaaat cactatacca ccggcgaagt catccgtttc
     3421 gaaaccaata ccagggatgc caggatcctc cactggagct tcggcgacaa ccagagtaac
     3481 gacacgaaac taccttccgc agtccatgcc tatgacgaac cgggggaata tacggtttcg
     3541 cttacagtaa atggagaatg caccgaatat gcgagggtct ctatcggtcc cgcgcccgtc
     3601 gtggaaaatc cacgcctggt acccagattc gtctacccgc agtccgccga agtcggcaag
     3661 ccggtccaat tctcggatac caccgttggc gcccagcaat ggcaatggcg tttcggagag
     3721 acagccacgg tcgacgctac ggacaaaagc ccccgctata cctataccac gcctggcttg
     3781 aagacggttt ccgtcgtgat caacaatgac gaaaggcaaa tgggcatttg caagatatac
     3841 gtcaatccgc cccctcctcc gaaaagaaag gaccatggcg gcaatgacag gcccatcatc
     3901 gtggtacatg acaagcccgg ccaggcgcct gctggcgaac agccatccac cgcgaaaacg
     3961 gagccggcgc ccgtgaaggc gccggatatt acaggccccg aattcgagac ccagctccgg
     4021 gccgtagcca ataaattcaa gaacgcagag agcttttccg cttatttctg cggcaacctg
     4081 aatacccagg tgagcctgaa cggacaaatg atacctttta cagaactgtg caacaagttc
     4141 ggctccctga agaatgaaaa gaaaattaag aaactgacgg tacaactggt caaaaacgaa
     4201 cagaccaatt gcatcatatc gatgttcgtg acccttaagg aaagggaagg cctgtttaat
     4261 aaaatttttt gagcatgaat gatttgacga tggatattca accgagccca gccttgcgac
     4321 aggcccttca catcgcgcaa gccgttgcaa gggagcacat gcatggggac ttcggcgccc
     4381 cccacctcct gaaggccctg ctgcacaggg atctgggcgt ggaagatcaa ttgtggaaaa
     4441 tggaccagga cgtctattac atagcggaat gggcggatgt acgccttgaa tcctgccgca
     4501 agacaccagc gccgaaagat gcccctgcag gcgatgagca ggtggctgcc attctggatg
     4561 aggccgataa tatccggctt cagctgaatg aggaagaaat atcccccatg cacgccctta
     4621 tcgctctcag cacacccgga gtggggttct ccttcgaaca gttgaagact ttcccgttgc
     4681 aaagggatgc cctgctgcaa tggcaacagg atcatgatat gctcaggaac ttatcgcaac
     4741 cggcatcccc ctccgggcaa ggccaacaag agcagcgcca gcgtggcacc caatcccttt
     4801 tgaagttttg ccgggagaag accctgcagg ccgcggatgg aacaatggac ccgatcatag
     4861 gaagggaaca ggagatccgc atgatggcgg agatcattag ccgtcgctct aaacccaatg
     4921 tcctgctcat cggagaacct ggcgtcggca agtccgcact ggtggacgga tttgcgtcgg
     4981 cgatccataa cggacaggtc ccggagtttt tacggcaggc caggctgttc gagctggaca
     5041 atggaacatt ggtcgccgga gcctcctaca aaggagaagt ggaggaaaga atcaaatcga
     5101 tcctgcagga gatcaagacc tttgataagg cgatactctt cattgatgag atacatacat
     5161 tactggaccg gcaaggtgcg gcagcgggag cggcgaacct gttaaagccg gagttggcaa
     5221 gagggaccat cactgtgatc ggagctacca ccctggaaga atacaggaag catatagaaa
     5281 aggatgaagc ctttgctcgt cgtttcgaat tgctgacggt ctccgaaccc gatgccacaa
     5341 ccaccttccg gatgatcagg acggtactgc cacggtatga agtacatcac cgggtccgtg
     5401 ccgatgacga aactttgaaa gcgacgatca ggctggcccg gcgttatatc aagggcagga
     5461 aactgcctga tgcagccatt gacctccttg accgttccat ggctgcattg cggctggcca
     5521 ttgacaccgg aatggccagc aaggaaaaat tcctgctgga attcgggcgg atgaaaaata
     5581 ccgccctgtg tgcagccccg gaagacctgc agtggcacta cggacaactg aaaaatgccc
     5641 tcggccccct gatatggacg gaagcggtct ccggaaccgc ctctggcgct gccaacgaag
     5701 cggcctccga agccgatctg ctcagcgaaa tatcctgcgt atatcagcgg cttgcggata
     5761 tccttccggt agagaggaca cagttgaacc aacaggatgt cgcctccatc gttgccgaca
     5821 agacagggat tcccatcggc aagctacaat cacaggaaca ggaccggctg ttgaatatgc
     5881 cggatgcgct gcggcaaaga ataatagggc aggatcacgc agtccggacg atctccgagg
     5941 cgatcctgga atcgaggtcc ggcctgagca agcccggaca gcccatcggg tccttctttt
     6001 ttctgggacc taccggcacc ggcaagactg aactcgccaa agctcttgct gaatttatgt
     6061 tccaggacga gaaattcatg atcaggttcg atatgtccga attcaaggaa gagcatgccg
     6121 ccgctcttct gtatggcgca ccgcccggtt atgtaggcta tgaggaaggc ggattactgg
     6181 tgaataagat aagacagcag ccatatgcgg tcgtcctttt cgacgaaatt gaaaaagcac
     6241 atgcatccgt gttcgacatt ttcctgcaga tcatggatga gggcaagctg cacgataagc
     6301 tgggacggga aggcgacttc tccaatgcca ttgtcatctt tacatccaac atagccagca
     6361 ggtatgttac ggaagcattt gcaaagggca gcacccccac gcatgccgaa ttgctggaaa
     6421 agatggcggc acatttccgg ccggaatttt taggaaggct tacggagatc gttcctttcg
     6481 ggcctgttga cccgcaaatg gtggaacata ttttctccat acaggtcgcg gctcttcatc
     6541 acctgttatc cgcacagggg atcgaactgc gaattacccc ggcggcggca aaacatgtcg
     6601 cgcaatcagg attttcccct gactacggag caagaccact ggccggcgtc atccgcagcc
     6661 acctgagaag gccgctgtcc cggatgatcg tcagcgggga attgcagcat ggaagtaaaa
     6721 ttacgatgga cctgggaccg gagcagcgat tgatctggct gcccggacag acataggata
     6781 tctttttatt aaaaattaaa acttcaaatt atggccattg aaccttatgg aatagggggc
     6841 acggaagtca ggacagatgc tttcgaagct tttgcagaca tcccgcaaaa ccgcgttttg
     6901 ctggcggaaa aactgaccaa ggaccctccc gtaaaacctg atatcgtcaa cgggcttaca
     6961 gatatcatct ccgtttttaa acacttcgat ccgaaggtgg acatcgagtt tgaatcggaa
     7021 gaaggcacaa tacgcgggga gacgcttcac tttcatggat tggaggactt tggcgccaaa
     7081 gggatcacgg cgcaaagccc ttatctgaaa gagctgaata tgaaaaaaca gcagtaccat
     7141 aagatcatcc ggcagctaaa gaccaataag ctgctcaaac aggcgatcgc tggtaaggaa
     7201 acaaaggaga acctgatcct cgccctgcaa tctctgatcc gggaattgga gacggcgcca
     7261 tgatcgattc aggcaccatg atcgattcaa tttattaaaa ctcaaacact ccctttatgg
     7321 cagttgaaca aactaaagca gtaaactcag aaaatccagt atacgagacg ctgaccaaac
     7381 cagcggaatc gctcgacggg cagttggtcg ggctggcaaa attcggagga ttcgacctgc
     7441 tggaatctgc ggtcgatgca gtacagaacc tcaatccatc ccgcaaggcc cggaaaaaga
     7501 tcttcctgga agagcaatcc aaaaaagaag agaggtcgga actgaaaaag acattgcagt
     7561 ggtgggtgga tatcctgcaa agctcagacg atatcacgga tatggtgaaa aactgcaatg
     7621 aaaaagcagc cgtagcggaa aagaccctca acaggaacct gaaacatgca gtctccgaaa
     7681 ctgccgaact ggaaaggtct taccgttcgg tcgccttgtt cttcaagaac accgaaagtc
     7741 tgaaagtcaa gaacgtctcg atcattaacg cggagccaga ccagctaaag gacctcgaca
     7801 atacccggtt cattgataca atccgcgtcg agttgatcaa taactatgat cgcctcgatc
     7861 tgcggaacaa ttacagcctg ctgatactcc ccggttacct ggggtccaac aaggtggtgg
     7921 aaaaatgggc caagatcgct tatgagaaca aggtaatgct gatcacggac tttgaacacc
     7981 tcgattcgcc ggatgacgtg atggaacttt tcgaagccgc taacctcacg ggaggcgacc
     8041 cctggcggtc caatgtcatc atgacatgca actggctggt gggccgtggc aaaatcgatg
     8101 agatcgaaga
//
LOCUS       DATGFG010000726         5409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_376690, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000726 DATGFG010000000
VERSION     DATGFG010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376690"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2375
                     /locus_tag="VKQ52_13560"
     CDS             <1..2375
                     /locus_tag="VKQ52_13560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88274.1"
                     /translation="FYTASVLDAAGLNDYSVAQFGTEVGWVPLMIDTATLYDQNSLTA
                     ASQKATSYTYSAIHYQPITATTTNSNGDTAVEYMSYPLDYIGLTGSDKFTQGILSLQN
                     ANIVTPAIEKYAQMKTSNGTFIGTMGSMLTSYNSQSLLPDTIWSTQYSKPSASFNPLS
                     ANNGTLTKDAAYIPQVVNSKYDLFGNVIEQQKINDLKHAYVWDYLGTYPIASVGNADS
                     ANIAYTSFEADGSGNWSIGSGAVDTTRGFTGRRCYQLSGSISKSGLTPSLTYLVSYWT
                     LNNSALTIPGTISGYPVKGKTIAINGANWTLYVHKVTGQSTVTVSGNGVAIDELRLYP
                     STAQMTTYTYDPLIGMTSQTDAGNRATYYEYDGLGRLKRVRDQDYNIVKTLEYQYQAT
                     TGCGSGCSVVPMQTLAGTKTIGYPVGVFNVNGKLLGNAAGPDDYVTKWNSDAADNGVG
                     TLTKGRDSLYFNLTLNSGQTVPAGVTGCRYYQYDLPWNVLDGVTANNADYVDFGDGTG
                     MRLPATVTDTPAVMPANTTRAGFFDQFTQQWWFIHSYADSSLKTLTFYHSDDTKTAAL
                     DNAYDPAVSLTKVRNLRGNLPQGTTQLGGSCYQQASALTVANITNWNSIGSVTGFWPH
                     CGDATTPCMNMSYAQDFMKNNRGLNTINTTQVNVYEAGYRDMSFKLSRLKSDWNTYFT
                     QLQDLEISDDHWDHEDLSALTHLKVFLLMATDQGHSNVHLNANDYTSNPPVTIPASVA
                     DGILNQIAAGAGQNVTNGFITILGNYGGRTSASNAAVQFLQSRGWTINVQ"
     gene            2420..4123
                     /locus_tag="VKQ52_13565"
     CDS             2420..4123
                     /locus_tag="VKQ52_13565"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HLZ88275.1"
                     /translation="MKRVFWILLLITGATRLSAQTVVNFRFTNGVVSSSDYLPNWIDV
                     HGDPSDSVITATDPVTGISISSIGTSNWFPNGDVCSADGIGEGGASYFPGSVMACAWM
                     QQGGIQAQFNAAVPQLVISGLSPDSVYYIRMSGSYLYGTDGDPTQYTVSGLNMLPYQD
                     LIVGGNTTTGITFQRVAPDANGKIRLYVNTTPTTDIAILSGLQIISGSAQIAMPVVHI
                     TSPVNLDVLAEETNIVINATATETGGTITKVEFYADTMKIGEADAAPYSTTWNNPNEG
                     HYLITAKATDGAGTTNTATINISVESLTSYWSMTGNIGMNPDSNFVGNVDSVRLAFRT
                     KNIERMTISAIGNIGIGTDSPTAQLHTTGTVRLAGLTGDSTKNRVVVSDSSGKLYYRN
                     VSSLNNRWLYNNGTLYDSVDNIAIGTSNPYNYKLAVNGTAIFTKAKVKTAGTWPDYVF
                     KKEYALPSLRELEKYITQHRHLPGIASEQDVQVNGIDLGDHAAALLKKVEELTLYLIQ
                     QNKATLEQEQKLAEQSRQMKVQQQQLTEQARQMAEQQARLDAQQKEIDELKALIKAKT
                     N"
     gene            4140..>5409
                     /locus_tag="VKQ52_13570"
     CDS             4140..>5409
                     /locus_tag="VKQ52_13570"
                     /inference="COORDINATES: protein motif:HMM:NF039454.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6443 domain-containing protein"
                     /protein_id="HLZ88276.1"
                     /translation="MRYKVMKKISVIGVALLGLTTAAIAQSNAPGSATQVPGPTGAVA
                     TTPPGYITGGQSPLVNYVRERDALGRITDTIIYPTAGYRDVRETTHYFDGLGRPLQTV
                     QRQASPGSSPVDLVTPVVYDPFGREVYKYLPYAASAGDPGDGSFKQDPINEQKNFYQN
                     TYPGEQPAYTGEQVFYGQTNYEASPLNRVTQSMAPGNSWAGSGIGVSMQYLVNSAADS
                     VEIWTIGTDTLNYQNNDITTNIPSAGGYYGAGQLYKTVTIDEQNHAVVEYKDKDGLVI
                     LKKVQIGTVAADYSGYSGWLSTYYVYDNLNQLRFVLSPKATRVVYGNNWNVSADTTTI
                     SELCFRYEYDGRKRMIAKKVPGAGWVYMVYDARDRLVYSQDANMRGRNQWMTTLYDVL
                     NRPTTTGMITYFGTANQLQAYVTANTGSGAI"
BASE COUNT         1375 a         1574 c         1362 g         1098 t
ORIGIN      
        1 cattttacac cgcgagtgtc ctggatgctg ctggacttaa cgactatagc gtcgcccaat
       61 tcggtacgga agtgggctgg gtccccctta tgatcgacac cgccacactt tatgatcaaa
      121 atagcctcac cgcagcttct cagaaggcaa cttcctatac ctatagtgcc attcactatc
      181 agccgatcac agctacgaca acgaacagca acggcgacac ggcagtcgaa tacatgagtt
      241 atcccctcga ctacatagga ttaaccggtt ctgataaatt cacgcaaggc atactttccc
      301 ttcaaaatgc caatatcgtc actcccgcga tcgagaaata cgcacaaatg aaaacgtcca
      361 atggcacctt cataggaacg atgggttcca tgttgacctc ttacaatagc cagagtcttc
      421 tgccggatac catctggtct acgcaataca gtaaaccttc agcttctttc aatcctttat
      481 cagcaaacaa cgggaccctg acaaaggacg ccgcgtatat cccccaggtt gtcaatagta
      541 aatatgatct ttttggcaac gtcatcgagc agcagaaaat aaatgatctt aagcatgcct
      601 atgtctggga ttatttggga acttatccca ttgcaagtgt tggcaatgcg gatagcgcaa
      661 atattgccta taccagtttt gaagccgatg ggtccggcaa ctggagcatc ggctcgggag
      721 cggtcgatac tacccgaggt tttacaggcc ggcggtgtta tcagctgtct ggttcgatat
      781 cgaagtctgg attgacccca tctctcacct atctggtctc ctactggaca ttgaacaata
      841 gcgctttgac catccctggc acgatctccg gttatccggt taaaggcaaa accatcgcga
      901 tcaacggagc taactggacc ctgtatgtgc acaaagtcac cggccagtcc accgttaccg
      961 tttccggaaa cggcgtggcg atcgacgagc tccgcctcta cccatccacc gcccaaatga
     1021 ccacctacac ttatgacccg ctcatcggca tgaccagtca gaccgatgcc ggcaaccgtg
     1081 ccacctatta tgaatacgac ggcctcggcc gcctgaagcg ggtgcgcgac caggactaca
     1141 atatcgtaaa gaccctggaa taccaatacc aggctacaac gggttgtggc agcggctgtt
     1201 ccgtcgttcc catgcagact cttgccggca ccaaaacgat cggttacccg gtaggtgtct
     1261 tcaatgtcaa cggaaaatta cttggcaatg ccgccgggcc ggatgactat gtcaccaaat
     1321 ggaatagcga cgcagcggac aacggtgtcg gtacgctgac aaaaggcagg gattccctct
     1381 attttaatct caccctgaac agcggacaga ccgtgccggc gggagtaaca ggttgccggt
     1441 attaccaata cgacctcccc tggaatgtcc tggatggtgt taccgccaac aacgcggact
     1501 atgttgactt cggggatggt accggtatgc ggctcccggc cactgttaca gataccccgg
     1561 ccgttatgcc cgccaacacc acaagagccg gcttctttga tcaatttacc caacagtggt
     1621 ggtttatcca ttcctatgcc gattccagcc ttaagaccct taccttctac catagcgatg
     1681 atacgaagac agcggccctg gacaacgcct atgacccggc tgtcagcctc accaaagtcc
     1741 gcaatttacg gggcaatctc ccacagggca cgactcagct cggcggctcc tgctaccagc
     1801 aggccagcgc actcaccgta gccaatatca ccaactggaa tagtatcggc agtgtcaccg
     1861 gtttttggcc gcattgcggt gacgcgacca cgccctgcat gaacatgagc tatgcccagg
     1921 attttatgaa aaataaccgg gggctcaata cgatcaatac gacccaggtg aatgtatatg
     1981 aagccggtta tcgtgacatg agctttaaat tgagtcgcct gaaatccgac tggaacacgt
     2041 atttcaccca gctgcaagac ctggagatat cggatgacca ctgggatcac gaagacctca
     2101 gcgcccttac gcacctgaag gtattcctgc tgatggccac cgatcaggga cattccaatg
     2161 tgcacctgaa tgccaacgat tatacgtcga acccgccggt gacgataccg gcttcggttg
     2221 ccgatgggat attgaatcaa atagcagccg gggccggtca gaatgtgacg aatggtttta
     2281 ttaccatact cggcaactat ggcggcagga caagcgcgag taatgcggca gtgcagttct
     2341 tgcagagcag gggctggacg atcaatgtac aataaacata aacaagtagt aataccccta
     2401 tagcaaaaat atgactagta tgaaaagagt attctggatc ctgctgctga tcaccggcgc
     2461 cacccggttg tctgcccaaa ccgttgtgaa ttttagattt accaatggtg tcgttagcag
     2521 cagtgactat cttcccaact ggatcgatgt gcatggcgac ccatccgatt ccgtgatcac
     2581 ggccaccgac ccggtgaccg gcatctccat cagttccatt ggaacttcaa actggtttcc
     2641 caatggggat gtatgctccg ccgacgggat aggagagggc ggcgccagct atttcccggg
     2701 atctgttatg gcttgtgcat ggatgcagca gggggggatc caagctcagt tcaatgccgc
     2761 cgttccccaa ttggtgatca gcgggctcag ccccgacagc gtatattata tccggatgag
     2821 tggctcctat ctctacggca ccgatgggga tcccacccag tatacggtgt ccgggctcaa
     2881 catgctgccc taccaggacc tgatcgtcgg aggcaatacg actaccggca tcactttcca
     2941 gcgggtggcg ccggatgcca atgggaagat tcgcttatat gtgaatacca cccctaccac
     3001 cgatatcgcc attctatccg gtctccagat catctccggc agtgcacaga tcgcaatgcc
     3061 cgtcgtgcat atcaccagtc cggtcaacct ggacgtgctg gccgaagaaa cgaatatagt
     3121 tatcaacgcc acagccacgg aaaccggcgg taccatcacg aaagtagagt tctatgccga
     3181 tacgatgaag atcggtgagg cggacgccgc accctacagc acgacctgga acaaccccaa
     3241 cgaagggcac tatttaatta cggcaaaggc gacggatggc gcgggtacca ccaataccgc
     3301 caccattaac atcagtgtcg aatccctcac ttcttactgg tctatgactg gtaacattgg
     3361 gatgaacccc gacagtaatt ttgtcggcaa cgtggacagc gtacgtctcg ccttccggac
     3421 gaagaatatc gaacgaatga cgatctccgc catcggcaat atcggcatcg gtaccgactc
     3481 tcccacggcc caactgcata ccacgggcac cgtccggctg gcagggttga caggcgacag
     3541 cactaagaac cgggtggtag tcagtgacag cagcggcaaa ctttactacc gcaatgtctc
     3601 gagcctgaac aaccgctggc tatacaacaa tggcacccta tacgattcgg tcgacaatat
     3661 tgccatcggc accagcaatc cctacaatta caagttggca gtcaacggca cggccatctt
     3721 taccaaggcc aaggtgaaga cggcgggcac ctggccggac tatgttttca aaaaagagta
     3781 tgccttgccc agcctgcggg aactggagaa atacatcacc caacaccgcc acctgcccgg
     3841 cattgcctcg gagcaggacg tgcaggtcaa tggcatcgac ctcggcgacc atgcggcggc
     3901 gctgctgaaa aaggtggaag agttgacgct ctacctgatc caacagaaca aggccactct
     3961 tgaacaggag cagaaactgg cggaacagag ccggcagatg aaagtccagc aacaacagtt
     4021 gactgaacag gcgcgtcaga tggccgaaca acaggcccgc ctggatgccc agcaaaagga
     4081 gatcgatgaa ctcaaggccc tgatcaaagc gaaaactaat taattcaacc ttctgcttta
     4141 tgcgatacaa ggtgatgaag aagatatcgg taatcggtgt agccctgttg ggactcacaa
     4201 cggctgccat cgcccaatcg aatgcccccg gctccgccac tcaggtgccg ggccccacgg
     4261 gcgccgttgc cacgacgccg ccgggctata tcacgggcgg ccaatccccg ctggtcaatt
     4321 atgtccggga acgggacgcc ctgggccgga tcaccgatac catcatctac cctacggcag
     4381 gctatagaga cgtccgggag acgacccatt actttgacgg cctgggccga cccctgcaaa
     4441 cggtgcagcg ccaggcctct cccggcagca gcccggtgga tctcgttact cccgtcgtat
     4501 acgatccctt tggccgggag gtgtataagt accttcccta tgccgcctcc gccggcgacc
     4561 ccggcgatgg tagtttcaag caagacccaa tcaacgaaca aaagaacttc tatcagaaca
     4621 cctacccggg cgagcaacct gcttatacgg gggagcaggt attctacggc cagaccaact
     4681 acgaggcgtc ccccctcaac agagtgacac aaagcatggc gcccggcaat agctgggccg
     4741 gtagtgggat cggtgtgtcc atgcaatacc ttgtcaacag cgccgccgac agcgtcgaga
     4801 tctggaccat cggcaccgat accctcaact accagaataa cgatataacg accaatattc
     4861 cctccgccgg cggctactat ggtgcaggtc aactctacaa gacggtcacc atcgatgaac
     4921 aaaaccatgc cgtagtcgag tataaggaca aggatgggct ggtgatcctg aagaaagtgc
     4981 agatcggcac ggtggccgcc gactactcgg gctacagcgg ctggctcagc acgtattatg
     5041 tgtatgataa tctgaaccaa ctccgtttcg tcctctctcc caaggccacc cgcgtcgtct
     5101 acggcaacaa ctggaacgta tccgccgaca cgacaaccat cagtgaactc tgtttccggt
     5161 atgaatatga cgggcgcaag cgcatgatcg ccaaaaaagt gccgggggca ggctgggttt
     5221 atatggtcta tgatgcccgc gaccggctgg tatatagcca ggatgccaat atgcgggggc
     5281 ggaaccaatg gatgaccacc ttgtacgatg tgctgaaccg gccgacaacc accggcatga
     5341 tcacctactt cggaacagcc aaccagttac aagcctatgt aaccgccaac accggcagtg
     5401 gcgccatca
//
LOCUS       DATGFG010000727         5044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_948469, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000727 DATGFG010000000
VERSION     DATGFG010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948469"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VKQ52_13575"
     CDS             complement(<1..651)
                     /locus_tag="VKQ52_13575"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HLZ88277.1"
                     /translation="MRSIMTRVQAAFIPAAILLLPFYVEAQYDMGITGSNYSGVRSML
                     VNPAAGADPRYQLDVNVLSASVLFNNTFLYVPKGAVPAFGFGSIIKGIIHTNKFATYY
                     DPARPGKLYNVTLSGEILGPSFQLALPNRQSIGLTIANRLSANVRDIPGATAENAYAY
                     LRSPGLWDTTFTDGSTRVNGMDWMEYGVHYAAVLFDDGTDQWNAGITLKYLQGIAAA"
     gene            808..2388
                     /locus_tag="VKQ52_13580"
     CDS             808..2388
                     /locus_tag="VKQ52_13580"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HLZ88278.1"
                     /translation="MRRSPIFFIILLPAAIIALLPLVTQAAPPAPHLPRIAIAGLGIE
                     SSTFSPALTEEPAFHAQYGAAVFDTYPFFAADSPLRRRAIWIPTITGHSLPGGAVTRA
                     AYESLVNKTLDSLRRHLPYDGLFFDIHGAMSVVGLDDPEGDFIIRIRKVVGKKTIIST
                     SMDLHGNVSFRLAENTDLITCYRMAPHEDAMDTKRRAVENLLTRIETGKGKPAYKAWI
                     PIPILLPGEKTSTRIEPGKSVYKAVAPAAAQPGIIDAAIWVGYAWADEPRNHAVVMVT
                     GDDREKVTQTAEQLAQGFWKVRSQYVFVAPTGTLQECLDSAAKSDKHPFFISDCGDNP
                     TAGGAGDVTWTIQQILLRPEFRSADGPALIYASIPGPGLVKAAIKAGVGATVDGYAGA
                     TVDHRYAPPVHLTGTVESIVPGDKDAEVETVIRVGSVHVIVTQKRKPYHKEVDFTRLG
                     LQPRKAAIVVVKIGYLEPELYAMRAAWLMALTPGGVDQDLSRLPYKRIQRPMFPLDDI
                     TKDPDLSARLVPSSDAAK"
     gene            2582..>5044
                     /locus_tag="VKQ52_13585"
     CDS             2582..>5044
                     /locus_tag="VKQ52_13585"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HLZ88279.1"
                     /translation="MTPKTRKRLFRFILVPILTLLVLVVIAITILFSQQERLVGMAVT
                     EINKKLPGELVVGGSELSVFQNFPYISIGLKNVKFLPGKAATDKPIYEAERMYVGFSL
                     PDILKQQYHIKVILLKNGHLDLVQDTTGELNIVEAARIAPDTTVKTTTTAKALDLDIK
                     KFVLKNMRVSWFDKVGGQHIVANIDRIQTAFRDEDGKIRADLKGDLVADYTRPHDTTL
                     FRHKHLETDIQFSYDQATRFLRLPVGKIKIGDAAFNLTGTVDLLHDNTVDLHITGDKP
                     DFRQLFAFAPEKVAKDLKHFRYDGYLSFDGSVKGKLKGGQQPLITLSFACKDAWLHNT
                     AANKKLDSLSFKGFYTNGAGHSLRTSELRLTDMTARPDKGIFTGNFILRDFTDPKILM
                     QVNSQLELGFFGAFLGIKDLERLSGHINLKMNFKELVDLSLPEKELSELTEGIQSELT
                     VTNLSFRAPSYAYTIEHLNLHANMKDGFVKLDSLSCKIGNSDFHCSASLDDLPALFHH
                     QEKPVNLTFNAHSNKIVFKELLSFDTVKARKAEEDEQIDGFNIDLALQTSVQELLHPN
                     PLPKGKLNISNLYASFVKYPHDFHDFGAELTINDTSVLLRNFAGSIDSSDIRFNGRLN
                     NYALWFEKVKKGKTLVAFDLRSQHLAVRDLVGRKGMQYLPQDLRQEVGSNIWLRTKTE
                     LRYDSTFQFANIRIANISGVLKNHNFQLDSISGNVKLGTDNFVKIDTLKGKIGNSDFN
                     VSMRLYTGKDTARRKKENYLQFASTLLDVDQLNNYVIAAEQEEQETLAAIHADTSAGS
                     AVATATTTASHDQGFNLFRIPFI"
BASE COUNT         1216 a         1622 c         1246 g          960 t
ORIGIN      
        1 ggcggcggcg atgccctgca gatatttgag cgtgataccc gcattccact gatcggtgcc
       61 gtcatcgaac agcacggcgg cataatgcac cccatactcc atccaatcca ttccattgac
      121 tcttgtactg ccgtcggtga acgtggtatc ccacagaccc gggctccgca ggtaggcata
      181 ggcattctct gccgtggccc cggggatgtc ccggacattg gcggagagcc ggttggcaat
      241 ggtaagcccg atggattggc ggttggggag cgccaactga aaggaggggc ccaatatttc
      301 cccggagagc gtcacattat acaatttccc cggccgggcc ggatcatagt acgtcgcaaa
      361 tttgttggtg tggataatgc ccttgatgat cgacccgaag ccaaaggccg ggacggcgcc
      421 tttgggaaca tacaggaagg tgttgttaaa aaggacgctt gccgacaata cattcacgtc
      481 taattggtat ctcgggtctg cgccggcggc tgggttgacc agcatactcc tgacgcccga
      541 ataattgctg ccggtgatgc ccatatcata ttgtgcctcc acataaaagg gcagcaggag
      601 aatggccgcc gggataaagg ccgcctgcac acgagtcata atggatctca tggctgtaaa
      661 tttttattaa gcaggaagat atcgggaaat tagcggcagg ccttggacag gatataaatt
      721 caaacgcaga acagaaggcg ggagttgcca ggacgcccga cttatgaaaa aatcttctta
      781 cattgatcac atcaaaaaca actcccggtg agacgatccc ccatattttt cattatcctg
      841 ctgccggcgg cgatcatcgc cctgctgccc ctggtgaccc aggccgcccc gcccgcgccc
      901 catttgcccc gcatcgccat cgccggcctc ggcatcgaat caagcacctt ctctccggcg
      961 ctcaccgaag aacccgcttt ccacgcacaa tacggcgccg ccgtcttcga cacctatccc
     1021 tttttcgccg ccgactcccc gctccgccgc cgcgccatat ggatccccac gataacgggt
     1081 cactccctcc ccggtggcgc cgtcacccgc gccgcctatg agtcgctggt gaacaagacc
     1141 ctcgactcgc tgcgcaggca tcttccctac gacggcctct tcttcgacat ccacggcgcc
     1201 atgagcgtcg tcggcctgga tgaccccgaa ggcgatttta tcatccgcat caggaaagta
     1261 gtgggaaaaa agaccatcat ctccacctcc atggacctgc acggcaatgt ctcttttcgc
     1321 ctcgcagaga ataccgatct catcacctgc tatcgtatgg cgccgcacga ggatgccatg
     1381 gacacaaaac gccgggccgt agagaacctc ctgacaagga tcgagaccgg caagggcaaa
     1441 cccgcctaca aagcctggat ccccatcccc atcctgctgc ccggggaaaa gacaagcacc
     1501 cgtatcgaac ccggcaagtc cgtctataaa gccgtcgcac ccgccgccgc ccaacccggt
     1561 atcatcgacg cagccatctg ggtgggctac gcctgggcgg acgaaccccg caatcacgcc
     1621 gtggtcatgg tcaccggcga cgacagggaa aaggtgacgc agacggccga acagctggcc
     1681 cagggctttt ggaaggtacg ttcgcaatat gtcttcgtcg ccccaacggg aaccctgcag
     1741 gaatgcctgg acagcgcggc aaaaagcgac aaacatccct tctttatcag cgactgcgga
     1801 gataacccca ccgccggcgg cgccggggac gttacctgga cgatccagca gatcctcctt
     1861 cggcccgaat tcagatccgc cgacggcccg gcactgatct acgcctccat ccccgggccc
     1921 ggcctggtga aagcggccat aaaggccggc gtcggcgcga cggtcgatgg ctatgccggt
     1981 gcaactgtgg atcaccgtta tgccccgccc gttcatctca ccggcacggt ggaatccatc
     2041 gtacccggcg acaaggatgc cgaggtggaa acggtcatcc gcgtgggcag cgtccatgtc
     2101 atcgtcaccc aaaagcgaaa accctatcac aaagaggtcg atttcacccg cctcgggctc
     2161 caaccccgca aagcggcgat cgtcgtggtc aagatcggct acctcgaacc cgagctctat
     2221 gccatgcggg cagcctggct gatggccctc acccccggcg gtgtggacca ggacctctcc
     2281 cgcctgccct ataagcgcat ccagcggccg atgttccccc tggacgacat cacgaaagac
     2341 ccggacctct cggcccggct cgtcccgtcc tccgacgcgg ccaaatagcg gctgacagcg
     2401 ggcaaacgga cgcctaagga gcggccaaat ggacgccaaa acagcggcta accagcgcct
     2461 aaccagcagc taaacggggg ctaaacaggg gttaaatatt aacgaatcct tcgcctcccc
     2521 gggatacttt atcttgcaaa tgatcgttta atttgtaccc cgatcgacaa gacaatttcc
     2581 tatgacgccg aaaacacgca aaagactttt ccgtttcatc ctggtcccca ttcttaccct
     2641 tctcgtactg gtcgttatcg ccatcaccat cctcttttcc cagcaggaac gcctcgtggg
     2701 tatggccgtg acggagatca acaaaaagct gcccggcgaa ctggtcgtcg gcggcagcga
     2761 actctcggta ttccagaatt ttccttatat ctccatcggg ctgaagaacg taaaattcct
     2821 gcccgggaag gctgccactg ataaacccat ctacgaggcc gaacgcatgt atgtcggctt
     2881 cagcctgccg gatatcctga aacagcaata ccacataaaa gtgatcctgc tgaaaaatgg
     2941 ccatctcgac ctcgtacagg acaccaccgg ggagctcaat atcgtcgaag ccgcgcgcat
     3001 cgcccccgac accaccgtta aaaccaccac cacggcaaaa gcgctcgacc tggacataaa
     3061 gaaattcgtc ctgaagaaca tgcgcgtctc ctggttcgac aaggtcggcg gacaacacat
     3121 agtggcaaat atcgaccgta tccagaccgc cttccgggac gaggacggaa agatcagagc
     3181 cgacctgaaa ggcgacctgg tggccgacta cacccgtccc cacgatacca ccctgtttcg
     3241 ccacaaacac ctcgagaccg atatccagtt ctcctatgat caggcgacca ggttcctgcg
     3301 cctgcccgtc ggcaaaataa agatcgggga tgccgccttc aacctcaccg gcacggtcga
     3361 tctcctccac gataataccg tcgacctgca tatcaccggc gataaacccg atttccgcca
     3421 attgtttgcc tttgccccgg aaaaagtggc aaaagatctg aaacatttcc gttatgacgg
     3481 ctatctcagc tttgacggat cggtgaaagg caaactaaaa ggcggccagc aacccctcat
     3541 caccctttcc ttcgcctgca aggacgcctg gctgcacaac acggcagcca ataaaaaact
     3601 cgattccctt tccttcaaag gattctatac caacggcgcc ggccactccc tgcgcacctc
     3661 cgagctgcgc ctgacagaca tgaccgcgcg gcccgataaa ggcatcttca ccggcaattt
     3721 catcctccgg gatttcacgg accccaaaat actcatgcag gtgaattccc aattggagct
     3781 gggcttcttc ggcgccttcc tcggcatcaa ggacctcgaa cgcctctccg gtcatatcaa
     3841 cctgaaaatg aattttaagg aactcgtgga tctcagcctg cccgaaaaag agctgagcga
     3901 actgacggag ggtattcaaa gcgaactgac cgtcaccaac ctttctttcc gcgccccgtc
     3961 ctatgcctac acgatcgaac acctgaacct ccacgccaac atgaaggatg gctttgtaaa
     4021 gctggattcc ctttcctgta aaatagggaa ctcggatttt cactgcagcg catcactgga
     4081 cgatctgccg gccctcttcc accaccagga gaagccggta aatctcacct tcaacgccca
     4141 tagcaacaag atcgttttta aagaactcct gtccttcgat acggtcaaag ccaggaaggc
     4201 ggaagaagac gaacagatcg acggtttcaa tatcgacctc gccctgcaga cctcggtaca
     4261 ggagctcctg caccccaatc ccctgccgaa agggaaacta aacatctcca acctctatgc
     4321 ttcctttgta aaataccccc acgacttcca cgacttcggc gccgagctca cgatcaatga
     4381 cacctctgtc ctgctccgga attttgccgg cagcatcgat agcagcgata tccggttcaa
     4441 cggccgcctg aacaactatg ccctgtggtt cgaaaaggta aaaaaaggga agaccctcgt
     4501 ggccttcgac ctgagatccc agcatctggc cgttcgcgac ctggtcggcc ggaaaggcat
     4561 gcaatatctc ccccaggatc tccggcagga ggtgggttcc aatatctggc tgcgaaccaa
     4621 aaccgaactg cggtacgaca gcaccttcca attcgccaat atccggatcg ccaatatctc
     4681 gggggttctc aaaaatcaca acttccagtt ggatagcatc agcggcaacg taaaactcgg
     4741 gacggataat tttgtaaaga tcgataccct caagggaaag atcggcaaca gcgacttcaa
     4801 tgtaagcatg cggctgtaca ccgggaagga caccgcccgc cggaaaaagg agaattacct
     4861 gcaatttgcc tctaccctgc tggatgtcga ccaactgaac aactatgtca tcgccgccga
     4921 acaggaggaa caggagaccc tggccgcgat ccacgcggac acctcggcag gctccgccgt
     4981 cgccacggcg acaaccaccg cctcccacga ccagggcttc aatcttttca ggataccttt
     5041 tatc
//
LOCUS       DATGFG010000728         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1268843, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000728 DATGFG010000000
VERSION     DATGFG010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268843"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..2076)
                     /locus_tag="VKQ52_13590"
     CDS             complement(172..2076)
                     /locus_tag="VKQ52_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HLZ88280.1"
                     /translation="MRKIYPFLLLSSCLFLTKHTLAQNSALNLTGSNYVTAGEDVINP
                     NGDFTMELWANIPQAMNDGNTHQLVSEGSFGTAFYIGYGADGTIQVGDFWGPTGIPMP
                     FGQWAHIALTYTYATSLTTLYINGKQVASSTSFAFTDDQPFRIGVQTDLSQPLTGRID
                     EVKAWSTPRTPSQVKADMFGVPAFNDNALVAYYKMDDGGGTLATNSAGTTGNSQDGAI
                     TGDAGGNNSWVASPIQYGNNGLVFDGVDDQVNIPAATGNAYDLAGGGTVEFWVNPTTL
                     TSSWSTIFGNRGAGGVRYSFHLSSTQIGLDNGTTINTLDYAVPAGTTNWTHLAFVMDG
                     VNTTTVYVNGILQGTITGTMGSSTGQPVTLGIAKNTSGADDRPFTGGIDEVRIWNTQR
                     TGSEILANRDNTLNGTESGLIGQFSFDQGISNGNDQGLTTAIDNTTLANNGTLANFAL
                     TGTTSNFTLHNLVAVPLPVTLTAFTANRSGNETVLQWQTATEENTRDFIIERSSDGKT
                     YTDIGATAAAGNSSSPRDYAWTDPAPGGDNNFYRLKQVDLDGNFAYSPVRVVSFPSAG
                     KLIWYLTGRSSAGILLQQGTNENWSLFDAGGRLLRTGQLSNGRTQLSQLPPGIYFVRV
                     ATNTISIALP"
     gene            2241..3059
                     /locus_tag="VKQ52_13595"
     CDS             2241..3059
                     /locus_tag="VKQ52_13595"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLZ88281.1"
                     /translation="MAIVKQISPLDDLRTISTAQRDEFRDNGHLVVRSVLSPEEAAAY
                     RQQVVEAVKRQSREKRKLPEKGIYGRAFGQMVNLWRLDEGVRQLVLSKRLGKVAADLL
                     GVANVRIYHDHALFKEAGGRATYWHQDQYYWPLDTADTVTMAMPMTDQSIEMGMFVFA
                     TGSHKHGTIMDPKLSSEPDGTYRKYIREHHFPLSRAAGMRAGDTTWHYGNTVHHTSAN
                     LSDRRREMMTITYMADGARVAQPVHPAQVSELNMWLNGLPPGRLAMSEFNPLVL"
BASE COUNT          730 a          810 c         1173 g          856 t
ORIGIN      
        1 tctgggttat ggagggatgg ggatcgacct ggagccgcat acggcggccg acgggaaggt
       61 ggagcggagt tttcagatct cgttcctgcc ggaccccgac ccgttgcagc tggcgatccg
      121 caagagttgg acgggggaat agaaaggggg gctgtcaggc ccccctttgt gttatggaag
      181 tgcgatactg atggtatttg ttgcaacgcg aacgaaatag atgccgggtg gcagctggga
      241 tagctgggtc ctgccgttcg acagctgacc tgtccgaagc aggcggccgc cggcgtcgaa
      301 gagggaccag ttttcgttag tgccctgttg caggaggatc ccggccgatg atctgcctgt
      361 gagataccag atgagtttcc cggcggaggg gaagctgacc acgcgaaccg gactataggc
      421 gaaattgccg tcgagatcga cctgtttcag ccggtagaag ttgttgtctc ctcccggcgc
      481 cgggtcggtc caggcgtagt cgcgtgggct ggagctgtta cccgcggccg cggtggcgcc
      541 gatatcggtg taggtcttcc cgtcgctgct cctttcgata atgaagtcac gggtattctc
      601 ttccgttgcc gtttgccatt gtaataccgt ttcgttaccg gagcggttgg cggtgaaggc
      661 ggtgagcgtc accggcaggg gtacggcgac aaggttgtgc agggtgaaat tggaggttgt
      721 accggtgagg gcaaaatttg ccagggtgcc gttgttggcc agggtggtat tgtcgatggc
      781 ggtggtgagg ccctggtcgt tgccgttgga gatgccctgg tcgaagctga attgtccgat
      841 caggccgctt tcggtgccgt tgagggtatt atccctgttg gcgaggatct cggagccggt
      901 gcgctgggta ttccagatgc ggacctcgtc tatgccgccg gtgaagggcc ggtcatcggc
      961 gccggaggta ttttttgcga tgccgagtgt taccggttga cccgtggagg agcccatggt
     1021 gccggtgatg gtgccctgga ggatgccgtt gacgtagacg gttgtcgtgt tgacgccgtc
     1081 catgacgaag gccaggtggg tccagttggt ggtgccggcg ggcacggcgt aatcgagggt
     1141 attgatggtg gtcccgttgt cgagtccgat ctgggtggag gagaggtgga agctatagcg
     1201 gacgccgccg gcgccgcggt taccgaatat cgtgctccag ctgctggtga gtgttgtggg
     1261 attgacccag aactcgaccg ttccgccgcc ggcgaggtca taggcgttgc cggtggcggc
     1321 ggggatattt acctggtcgt cgacgccatc gaatacgagc ccgttgttgc cgtattgtat
     1381 gggcgatgcc acccaggagt tgttgccgcc ggcgtcgccg gtgatggcgc cgtcctggct
     1441 gttgcccgtg gttccggcgc tgttggtggc cagggttccg cctccgtcgt ccattttgta
     1501 gtaggcgacg agggcgttgt cgttaaaggc tgggacgccg aacatatccg cttttacctg
     1561 ggagggggtc ctgggggtgc tccaggcttt tacctcatct atccggccgg tgaggggctg
     1621 ggacaggtcg gtctgtactc cgatgcggaa gggctggtcg tcggtgaagg cgaaactcgt
     1681 gctggaggcc acctgtttgc cgttgatgta cagcgtggtc aatgaagttg cataggtata
     1741 ggtcagggcg atatgggccc attggccaaa gggcattggg atgcctgtcg gtccccagaa
     1801 gtcgccgacc tggatcgtgc catcggcgcc atagccgatg taaaaggctg tcccgaacga
     1861 tccttccgac accaattgat gggtattgcc atcgttcatt gcctggggta tattggccca
     1921 gagctccatg gtaaaatcgc cattggggtt gatcacgtct tcaccggcgg tgacgtaatt
     1981 ggatcctgta agattcaatg cggagttttg tgcaagagtg tgtttggtca ggaagaggca
     2041 agacgatagc aggagaaaag ggtaaatttt tctcatagga tacagatttt atgtgcggga
     2101 tcaaggtagc ctaaaagggt gaggcgagga aatcggccgg gtcatttaac ggggctctag
     2161 gggttactcc tgatgccgtg gggcggcagt tttctataaa gaatttccat ttttgaaagg
     2221 atgcgttaat ttacagggag atggccattg tgaagcaaat atcaccactg gatgatctcc
     2281 ggactatctc tacggcgcag cgggatgagt tccgggacaa cgggcatttg gtggtgcgga
     2341 gtgtgctgag tccggaggag gcggcggctt atcgccagca ggtggtggag gcggtgaagc
     2401 ggcagagccg ggagaagcgg aagttgccgg agaaggggat ctatggccgg gcttttgggc
     2461 agatggtcaa cctgtggcgg cttgatgaag gggtgcggca gctggtgttg tcgaagcggc
     2521 tgggcaaggt ggcggccgat ctgctgggag tggccaatgt ccggatctat catgatcatg
     2581 ccttgttcaa ggaggcgggt gggcgggcta cctattggca ccaggatcag tattactggc
     2641 cgttggacac ggccgatacg gtgacgatgg cgatgccaat gacggaccag agtatcgaga
     2701 tggggatgtt tgtctttgcg acgggttcgc ataagcatgg tacgatcatg gaccccaagc
     2761 tttcttcgga gccggatgga acgtatcgaa aatatatccg ggaacatcat tttccgctga
     2821 gccgggctgc ggggatgcgg gcgggtgata cgacctggca ttatgggaat acggtgcatc
     2881 acacgtccgc caatttatcc gaccggcgga gggagatgat gaccattacg tatatggccg
     2941 atggggccag ggtggcgcag ccggttcatc cggcgcaggt aagtgagctg aatatgtggt
     3001 tgaacgggtt gccgcccggg aggctggcga tgtcggagtt caatccgtta gttttgtagc
     3061 taattcgtaa ccagggccta gtagttttac cccaaaaacg ggtgtcatcc cgcctgaaaa
     3121 aaatacacta aaaagggtat ataccgtttg gggaaaattt cccaatttag cattcgaatc
     3181 taaaaataat caccaaataa tttgaaaaat agaaaacgaa tatgtacctt cacgctacta
     3241 ttaacgcctg ccatattcta aaatcctctg aatattaggc taattaactc tttcatgtat
     3301 accttttgat tgacccacca atccttgaaa ttcctcaaga tcttgtagat ctggaattag
     3361 taagaaagcc ataatccaca ttgctatttt gtaaagcctg cttctctttt gtcagaagta
     3421 ttttctagaa gccaatacct cgtgttagcc tgactatttc catggccatc cttgaaagat
     3481 caagtatcta cttgaaccct aaaaatgccc agtggtaatg aaaaaacaaa tattggctat
     3541 agatgatagc aaagctatcc ggttcctgc
//
LOCUS       DATGFG010000729         5535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_13113, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000729 DATGFG010000000
VERSION     DATGFG010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13113"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..467)
                     /locus_tag="VKQ52_13600"
     CDS             complement(108..467)
                     /locus_tag="VKQ52_13600"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ88282.1"
                     /translation="MPMYPARRCPASYTAAITNNITKTGVAAGDITTYLASRGTLTGA
                     NALQRIIEEKRIAHLLSPEDYVDWRRTGWPALTIVQNAQTSAIPRRLTYPQSEINSNP
                     QPQQSATLTDRVWWDAP"
     gene            complement(549..3239)
                     /locus_tag="VKQ52_13605"
     CDS             complement(549..3239)
                     /locus_tag="VKQ52_13605"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HLZ88283.1"
                     /translation="MNKRRLLPILILSVILLRTTAQTTETATITSEGNLAVVAAPTGS
                     GRPGAALPALNDGETPTPPGNARYPRPPRQGNQWVQYEWAQPVTTRQVAVFWWDYSHL
                     AKLPSAYHIEYWDGTAFQPVHNTAGLGLESGKYNPTSFDEVTTTRLRLVMDSSDRYSS
                     TLAEWTVAESPNSHPIAPLVMAGADRDVMLDGKTWLSGHTRSVHPVTRVEWTKTTGPG
                     AVNISDPAAADATATFSAPGVYRLGFTAWQGDLHSSGEVRVKVVIPPPAKRLDVVYTK
                     HYSIDSRLWNDRAKVIITNWIPWCIAQCERTDLSTGEGGLDNFIEAAKALRGEPHGKH
                     KGYVFSNAWIHQTVEAMCEALMVDPKGDTAIIAAQERMKTTLERWIPIILAAQEPDGY
                     LQTAWTLRDTSRWHHRWSPEGRGNHEGYVSGYFIESAINHYTLTEGRDKRLYNAAKRL
                     ADCWVANIGPGKIAWYDGHQEMEQALVRFGRFVNDMEGQGHGDAYIRLAKFLLDNRKG
                     GSEYDQSHVPVQEQYEAVGHAVRATYTYSGMADVAAETKDVDYQSAVLSLWDNMVNRK
                     YYITGGIGSGETSEGFGPDYSLRNNAYCESCSSCGLIFFEYKMNLAYHDAKYADLYEE
                     TLYNALLGSLSLDGKDFTYTNALSTAQPRYGWHVCPCCVGNIPRTLLMMPTWTYVRGE
                     DGLYVNLFIGSTIRVEKLAGTDVEMVQQTDYPWNGRVGMTVNPAKPATFAVYIRIPNR
                     TTSELYTPQPQVNGLVSLSVNGQPLTPSIEHGYAVIRRTWKKGDKIAFELPMAIQQVT
                     ADDRIEADRGRVALRYGPLLYNVEAADNKDISQAIGSEPLTLQWDNDLLHGVMVIKGR
                     WADGSPLLAIPNFARLNRLPPTETPESASKPPISIVWIKK"
     gene            3417..4361
                     /locus_tag="VKQ52_13610"
     CDS             3417..4361
                     /locus_tag="VKQ52_13610"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLZ88284.1"
                     /translation="MASSSKVVFFIPPGVHMLDMSGPSQAFLGAGQMVSGYPIEYCSF
                     REEVTDSTGLRLSRLNRYDAVSLRAGDYLFVPGFSSHLLVAGEDRKEWEGVYAWLQRE
                     AAKGVRVCSVCTGAFVLARAGLLAGKTCTTHWSMIPLLRREFPDIDIREDVLFTEEGN
                     LYTSAGISSGIDLALYLLEADQGPLLAHKVARELVVYFRRGGRHSQESVYLNYRNHLH
                     RGIHELQDWLIGHLDKRTTIDQMANMVNMSSRNLTRTFKVQTGISINRYITLLRLELA
                     ASLRYSPGITMRDIASRCGFANERQLQRIMKSPVVRLQ"
     gene            4405..5178
                     /locus_tag="VKQ52_13615"
     CDS             4405..5178
                     /locus_tag="VKQ52_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLZ88285.1"
                     /translation="MRIGFIGVGRIACAVVEGLCTAEPTPEDLELFLSPRNEESSRAL
                     AARFAHVSRLESNQEVVDRSEILIISVRPPVAAEVLGALRFREEQTVVSLVALLPFAR
                     LRELTRPAAVVCRAIPLPTVVQHNCPIPLYRADERVVRLFGHLGETLPVEDEAGLHAL
                     WTLTGLITPYYDLLTRLSEWTVAHGVEPATASAYVANMFQSLSFLAQQSRPIDFGELA
                     RHAATPGGINEQAGKEISAAGAHNVWAAAAERVLQRFDR"
     gene            5353..>5535
                     /locus_tag="VKQ52_13620"
     CDS             5353..>5535
                     /locus_tag="VKQ52_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88286.1"
                     /translation="MFGNYFKTAWRNLWKNKVYSIINIAGLAISMAACIVIMLFVFYE
                     KSFDHFHTKNIYRLNEV"
BASE COUNT         1028 a         1422 c         1838 g         1247 t
ORIGIN      
        1 tgaatgcggg agctgtctcc acctacgtct ttccattata aaatctttca attataagag
       61 tgagggccgt gagtctaata gtctccggcc ctttctcttt atctattcta tggtgcgtcc
      121 caccagaccc ggtcggtgag ggtcgccgac tgctggggct gcgggttgga gttgatctcg
      181 gattgcggat aggtaaggcg gcgcggtatc gcggaggttt gcgcattctg gacgatggtg
      241 agcgcgggcc atccggtgcg gcgccagtcg acatagtctt ccggcgagag caggtgtgcg
      301 atgcgtttct cttcgatgat gcgttgcagg gcattggcgc cggtgagggt gccgcgcgag
      361 gcgagatagg tggtgatatc gcccgcggct acacctgttt ttgttatgtt gttggtgata
      421 gcagccgtat aagaggctgg gcatctcctg gcagggtaca taggcattgg gagacagttg
      481 ggcgaaagtc gttgtaggat agtacaggat ggtcaatagt aacctgattt taacttaaat
      541 caaaaaggct atttctttat ccatacgatg gagatgggcg gcttgctggc agattccgga
      601 gtttctgtcg gcggcaggcg gttgagtctt gcgaagttgg ggatggccag caacggagag
      661 ccatcggccc aacgtccttt tataaccatg acgccgtgca ggagatcgtt atcccattgc
      721 agggtgagcg gctcggagcc gatggcctgg ctgatgtcct tgttatcggc ggcttcgaca
      781 ttatacagga ggggaccata gcgcagggct acgcggccgc gatcggcttc gatcctgtcg
      841 tcggcggtca cctgttgaat ggccatgggt aattcgaatg cgatcttatc gcctttcttc
      901 catgttctgc ggataacggc gtagccgtgt tcgatggaag gagtgagcgg ttgtccgttg
      961 accgaaaggg aaacaagacc gttcacctgc ggctgtgggg tgtagagctc gctggtggtc
     1021 cggttgggga tgcggatata gacggcgaaa gttgccggtt tggcggggtt gacggtcatc
     1081 ccgaccctgc cgttccaggg atagtccgtc tgctgcacca tctccacgtc cgtgcctgcc
     1141 agtttctcca ccctgatggt gctgccgatg aagagattga catagaggcc gtcttccccg
     1201 cgtacatagg tccaggtcgg catcatgagc agggtgcggg ggatattgcc gacgcagcag
     1261 gggcagacat gccagccata acggggttgc gcagtcgaca gggcattggt ataggtgaag
     1321 tctttgccgt cgagggaaag ggatcccagg agggcattgt agagggtctc ctcgtaaaga
     1381 tcggcgtact tcgcgtcgtg gtaggccagg ttcatcttgt attcgaagaa gatgagcccg
     1441 caactggagc aggactcgca ataggcgtta ttgcgcaggg aatagtcggg gccgaagcct
     1501 tcggaggtct caccgctgcc gatgccgccc gtgatataat atttccggtt gaccatatta
     1561 tcccagagcg agagtacggc gctctggtaa tccacgtcct ttgtctcggc cgcgacatcg
     1621 gccatgcccg aataggtgta ggtggcgcgt acggcgtggc cgacggcctc atattgttcc
     1681 tgtacgggga catggctctg atcgtattcg ctgccgccct tccggttgtc gagcaggaat
     1741 ttggcgaggc ggatataggc atcgccatgc ccctgtcctt ccatatcgtt cacaaaccgg
     1801 ccaaagcgga cgagggcctg ttccatctcc tggtgaccgt cgtaccatgc gatcttgccg
     1861 gggccgatat tggcgaccca gcaatcggcg aggcgtttgg cggcgttata caggcgttta
     1921 tccctgcctt ccgtcagggt atagtgattg atggcggact cgatgaaata gcccgagaca
     1981 tatccttcgt gattgccgcg gccttcgggt gaccagcgat ggtgccagcg gctcgtatcg
     2041 cgcagggtcc aggccgtctg gagatagccg tcgggttcct gtgcggcgag gatgatcggt
     2101 atccaccgtt cgagtgtggt cttcatcctt tcctgcgctg cgatgatcgc ggtatcgcct
     2161 tttggatcga ccatcagggc ttcgcacatg gcttccacgg tctggtggat ccaggcattg
     2221 gaaaagacat agcccttgtg ttttccgtgg ggttcgccgc gaagcgcttt tgcggcttcg
     2281 atgaaattgt cgaggccgcc ctcgccggtg ctgaggtcgg tgcgctcgca ctgggcgatg
     2341 caccagggga tccagttggt tatgatgacc tttgcccgat cgttccagag ccggctatcg
     2401 atgctgtaat gttttgtata gacaacatcg agtcttttgg cgggaggcgg gatgacgact
     2461 tttacgcgta cctcaccact gctgtggaga tctccctgcc aggcggtgaa gccgagccgg
     2521 taaacgccgg gggcggagaa ggtggcggtg gcatcggccg cggccggatc gctgatgttg
     2581 accgcgccgg ggcccgtagt ttttgtccat tccacccgcg tgacaggatg caccgaccgg
     2641 gtgtggccgg agagccaggt cttgccgtcc agcatcacgt cgcggtcggc gcctgccatc
     2701 accagcgggg cgatggggtg ggagttgggg gactcggcga cggtccattc ggcgagcgta
     2761 gagctgtagc gatcgctgga gtccatcacg agacgcaggc gcgtggtcgt gacctcgtcg
     2821 aagctggtgg gattgtattt cccgctttcg agcccgagcc cggcggtatt gtgcacgggc
     2881 tgaaaggctg tgccgtccca gtattcgata tggtaggcgg aaggcagttt tgccaggtgg
     2941 ctgtagtccc accagaaaac ggccacctgc cgggtggtga caggttgggc ccactcatac
     3001 tgaacccact ggttgccctg gcggggaggc cgggggtagc gggcatttcc cgggggagtg
     3061 ggggtctcgc cgtcattcag ggccgggagc gcggcgccgg gccggcccga gccggtgggg
     3121 gccgcaacaa cagcgagatt gccttcgctg gtgatggtgg ctgtctccgt tgtctgtgcg
     3181 gtggtccgca ggaggatgac ggagaggatg aggatcggca gaaggcggcg cttgttcatg
     3241 tgaatgacgg agtttgggtt aaatgtacgg tggacggccg acacgggctt atccataatt
     3301 gactcaaacc aacccgattt tgactgttgg tggagtgggt ggcccgatct gtcgccctac
     3361 tgtccttgtt gcgatgggcg ggtggagtta tttttgggga aaataactcc tgttttatgg
     3421 cttcctccag taaagtcgtc ttctttatac cgcccggcgt ccacatgctg gatatgtcgg
     3481 gtccaagcca ggccttcctg ggcgccgggc aaatggtgtc gggttatccc atcgagtact
     3541 gttcgttccg ggaggaggtc accgactcca cgggactccg cctgtcgcgc ctgaaccgct
     3601 atgatgcggt gagcctgcgg gccggggatt atctcttcgt gccgggcttt agcagccatc
     3661 tgctggtggc gggtgaggac cggaaagagt gggagggggt gtatgcctgg ctgcagcgcg
     3721 aagcagcgaa gggggtgcgg gtctgttcgg tatgtacggg tgcgtttgtg ctggcccggg
     3781 cggggttgct ggccggcaag acctgtacca cgcactggag catgatcccc ctgttgcgcc
     3841 gggagttccc ggatatcgat atccgggagg atgttctgtt cacggaggaa gggaatctct
     3901 ataccagcgc gggtatcagc tccgggatcg accttgccct ctatttactc gaggccgacc
     3961 aggggccctt gctggcgcat aaggtagccc gtgagctggt ggtctatttc cggcggggcg
     4021 gccggcattc gcaggaaagc gtctacctga actatcgcaa ccacctgcac cggggcatcc
     4081 acgaactgca ggactggctg atcgggcatc tcgacaaacg gacgacgatc gaccagatgg
     4141 cgaacatggt caatatgagc agccgtaacc tgacgcggac cttcaaggta cagacgggga
     4201 tctccatcaa ccggtatatc accctgttac ggctggagct ggcggccagt ctccgctact
     4261 cccccggtat cacgatgcgc gatatcgcca gccgttgtgg ttttgccaac gagcggcagc
     4321 tgcagcggat aatgaagagc ccggttgtcc ggctccagtg atggtccggc attagtggct
     4381 atatttggcg caaaaaagaa caacatgcga atcgggttta tcggcgtggg caggattgcc
     4441 tgcgcggtgg tggagggttt gtgcacggcg gagcccacgc cggaggattt ggaacttttt
     4501 ttatctcccc gcaatgagga atcgtcgagg gccctggcgg cgcggtttgc gcatgtcagc
     4561 cggctggaga gcaaccagga ggtggtggac aggagtgaga tcctgatcat ttcggtgcgg
     4621 ccgccggttg ccgcagaggt attgggtgcg ttgcggttcc gcgaagaaca gaccgtggtg
     4681 tcgctggtag ccttgttgcc tttcgcccga ttgcgggagc tgacccggcc ggcagccgtt
     4741 gtctgccggg cgatacccct gcccactgtg gtgcagcata actgtcccat tccgctctat
     4801 cgcgccgatg aacgggtggt ccgcctgttt ggccatctcg gcgagaccct gccggtggag
     4861 gacgaggcgg gcttgcatgc gctatggacg ctcacgggct tgatcacgcc gtattacgac
     4921 ttgttaacgc ggctgagcga gtggaccgtc gcgcatggcg tggagccggc aacggcgagt
     4981 gcctatgttg cgaatatgtt tcaatccctg tcctttttgg cgcagcagtc gaggcccatc
     5041 gatttcgggg agttggcgag gcatgcggcc acgccgggtg ggatcaacga gcaggcaggg
     5101 aaggagatat ccgcggcggg cgctcataat gtgtgggccg cggcggcgga gagggtgttg
     5161 cagcgattcg atcgctgatg gcaaaaaaat attcaacaag gacaggcata gaatgaaggc
     5221 acgcataacg aaacagtggt tgtatcaaaa ccggacatcc ggcatcccga aaagaataca
     5281 actaattgat tagtagttag tggaaaaatt ggcaccacgc tggcagtacc attgaaaatc
     5341 aaagcactgc caatgttcgg gaactatttc aagactgcct ggcgcaatct gtggaagaac
     5401 aaggtctact ccatcatcaa tatcgccggc cttgccatta gcatggccgc ctgtatagtg
     5461 atcatgctct ttgtgttcta tgagaagagc tttgaccatt ttcataccaa aaacatctac
     5521 cggctcaacg aggtg
//
LOCUS       DATGFG010000730         5289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_677875, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000730 DATGFG010000000
VERSION     DATGFG010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677875"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VKQ52_13625"
     CDS             complement(<1..502)
                     /locus_tag="VKQ52_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88287.1"
                     /translation="MRSFFIDIFRRNIYLLLAAFVLFISGYFLNLYFSSDASVSVLRN
                     SIQSSLQQRQNDFTRLTKDTAYLYRLAGNHYSKADLETVLKKDYGVFLFGTDSAGNPG
                     RLQFWNDQRSLPSGAILNGPDGTGFTQLSNGQYDYVRVTVPTAAGHPMIAIALIPIRW
                     QYFISVS"
     gene            complement(545..1441)
                     /gene="mazG"
                     /locus_tag="VKQ52_13630"
     CDS             complement(545..1441)
                     /gene="mazG"
                     /locus_tag="VKQ52_13630"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HLZ88288.1"
                     /translation="MSDNITAFQRLVKIMDELREQCPWDKKQTIQTLRPLTIEETYEL
                     ADAITDNDWKGIKEELGDLFLHLVFYARIGKEQGQFTLDEVLNAICDKLIARHPHIYG
                     DTSALPGLVPVNNEEDVKRNWEQLKLREGKKSVLSGVPVSLPATVKATRLQEKAGKVG
                     FEWDETAQVWEKVREEMAELQEAITLGDPDHIEEEFGDLSFSLINYARFLHIDAENAL
                     ERTNKKFIARFTRMEEEARSRGRSLADMTLAEMDDLWNAIKKAEPSGPQPSAAKVATE
                     EERGEAAPTKANRHERKAGEAG"
     gene            complement(1551..2813)
                     /locus_tag="VKQ52_13635"
     CDS             complement(1551..2813)
                     /locus_tag="VKQ52_13635"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HLZ88289.1"
                     /translation="MSHLKERKEKNCLNCQTEVIGRYCHKCGQENLEPKETVWHLVQH
                     FFNDITHFDGKFFDTVRYLIRKPGFLSIEYMRGRRMAYLNPIRMYVFTSAIFFIILFT
                     ISKPEDMAKTMREDKTEQQQKTRNLADLRADTARLRNRLASAEDEDDKNDYRERIEFR
                     NIEITSIKKAYGDSAKQLFSNRELDSILAAAYADSLAGGSLDIAKSKKFRRAMRNFSN
                     QAATGETTNFNFMGSDIDYKTVEQYDSAEHALPDSLRDGWIKHFITRRGIVIKEEWRD
                     DKRLFVEHLLENIFHSFPKILWISLPIFALILNILYFRHKQYFYVNHGIFTIHVYCAT
                     FIQLFLAMMLFTIRKAATAQWLHIILDLANFAIFLWMLIYLYKAMRGFYGQRRAKTFV
                     KYFITMGLMFFINLILLCLFLLISVFSV"
     gene            2912..3757
                     /locus_tag="VKQ52_13640"
     CDS             2912..3757
                     /locus_tag="VKQ52_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88290.1"
                     /translation="MKVVVGCLLGLALGGSVHAQYLYKDLVVTRQNNARWKLFEENHV
                     KSVTLKSLEADGRPTEGFLGEQEVAGDYSQMVTHTQTSGSTDSWLISQYANGRMVKNT
                     DTSDTYQSVTDYTYDTGGHIMSITNTSVETDNHLRDVEQHIWSYDPAHPGRPLSMLKI
                     KNGTDTTRITFVTDDKGNIAEERSTRLGEALPTIYYYYNEDNLMTDIVRYSIRAKRLL
                     PLDMFEYEEGRLRSMLVVPEEGNTFYQKWYYTYDEEKGLKTKDICYNKQKELLGTIEY
                     VYSYK"
     gene            3814..4446
                     /locus_tag="VKQ52_13645"
     CDS             3814..4446
                     /locus_tag="VKQ52_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88291.1"
                     /translation="MRRLIFLSLVLCGLSGMAQDLDFPDFRNKKDNFSKISDKDVRAD
                     VASFALAGVDESIGKAPLTEVPVVDFGPNFIKYSNDNIQVTIKTGVFFPSKHKLMMQE
                     GHLLRIDSKPYYGGIIGQPPHTTIESITVLVGKDTVAIPPTAFFDLYDPKFSFNQNGA
                     TRSRNGVFLSNDKHTFYIYLLNIDNKGIEYTFVIRDKQYLRRVVDWGFLN"
     gene            complement(4443..>5289)
                     /locus_tag="VKQ52_13650"
     CDS             complement(4443..>5289)
                     /locus_tag="VKQ52_13650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ88292.1"
                     /translation="VMGYVQMAFAVSQVAGIPLGLFLANRFGWHTPFRMIVGFCLPVG
                     LVILRWMQPVADHLQQRARQRPFPHLLRTVSQKRYLRAFLSTVFLSTGGFLLMPYSST
                     FLVHNVGVGEPILPIVFIVAGAAGLVTGPLIGKWSDRIGKFRMFIDGSIFATIMVQIL
                     THLSVTPLWEVLLINTLMYTAVFSRMIPSQALISAVPDGPDRGAFMSINSSVQQLGGG
                     IASAMGGFIISQDATGRLLHYDTLGFVTVGAFMICAIFMYGVDKQLAAKSAGQPLQGQ
                     TVDAA"
BASE COUNT         1147 a         1238 c         1669 g         1235 t
ORIGIN      
        1 tggagaccga gataaagtat tgccagcgga tagggatcag ggcgatggcg atcatggggt
       61 ggccggccgc ggtgggcacg gttacgcgga catagtcgta ctggccgttg ctcagttgcg
      121 tgaagccggt gccgtcgggg ccgttgagga tggcgccgga gggtagggag cgctggtcgt
      181 tccagaattg gaggcgaccg gggttacccg cggagtcggt gccaaagagg aagacgccat
      241 agtccttttt gaggacggtt tcgaggtcgg ccttggaata gtggttgccg gcgaggcggt
      301 agaggtaggc ggtgtccttt gtgaggcggg tgaagtcgtt ttgccgttgc tgcagggagg
      361 attggatgga gttgcgcagg acggagaccg aagcatcgct gctaaagtag agattgagga
      421 agtacccgct gatgaacagc acgaaggcgg caagtaagag gtagatgttc ctacggaaaa
      481 tatcgatgaa aaaggatctc aatccgagag tatttttgtt ttttggccag ggccgttcat
      541 ccagttagcc ggcttcgccg gcttttcgct catgtctgtt ggccttcgtc ggagctgctt
      601 cgccacgctc ctcctcggtc gcaaccttcg ctgcgctcgg ttgtggacca gaaggctccg
      661 ccttctttat ggcattccag aggtcgtcca tttccgcgag ggtcatgtcg gccagggacc
      721 tgccccggga acgggcctct tcttccatgc gggtgaaccg ggcgatgaat ttcttattgg
      781 ttcgctccag ggcgttctcc gcgtctatat gcaaaaaccg tgcataattg atcagggaga
      841 aggaaagatc gccgaattct tcttcgatat ggtcggggtc accgagggtg atggcctcct
      901 gcagttcggc catttcttcc cggaccttct cccacacctg ggccgtttcg tcccattcga
      961 agccgacctt tccggctttt tcctggagcc gggtcgcttt tacggtggcg gggagggaga
     1021 cggggacgcc gctgaggacg gatttcttgc cttccctgag ctttagttgc tcccagttgc
     1081 gtttgacgtc ctcttcgttg ttgacgggga cgaggccggg aagggcggag gtgtcgccgt
     1141 agatatgggg gtggcgggcg atgagtttgt cgcagatggc gttgaggacc tcgtcgaggg
     1201 tgaactgccc ctgttctttc ccgatacggg catagaagac gaggtgcagg aagagatcgc
     1261 cgagttcttc ctttataccc ttccagtcgt tatcggtgat ggcgtcggcc agttcgtagg
     1321 tttcttcgat ggtgaggggg cggagggtct ggatcgtttg ttttttgtcc caggggcatt
     1381 gttcccgcag ttcgtccatt attttgacca ggcgctggaa ggctgttatg ttatcactca
     1441 tatattgctg cttcttgttt tcgtgggatg ctgcgcatcc cggatcaagg gctaatatct
     1501 gtcctttgga cggaatgcct ccggcattcc cgaggcctcc cctaccctac ctataccgag
     1561 aatacggaga tcagcaggaa gaggcacagc aggatcagat tgatgaagaa catcaagccc
     1621 atcgtgatga aatatttcac gaaggtcttt gcccttcgct gcccgtagaa gccccgcatc
     1681 gccttataga ggtagatgag catccacagg aaaatggcaa agttcgccaa atccaggata
     1741 atatgcaacc attgggcggt ggcggctttt cggatggtga agagcatcat cgccaggaag
     1801 agctggatga aggtggcgca gtagacgtgg atggtgaaga tgccgtggtt gacgtaaaaa
     1861 tattgtttgt gcctgaagta taaaatgttc aggatcaggg cgaagatggg cagggagatc
     1921 cataggatct tggggaagga atggaagatg ttctcgagga ggtgttcgac gaagaggcgt
     1981 ttgtcgtccc gccattcttc cttgatcacg atgccgcggc gggtgatgaa gtgcttgatc
     2041 cagccatcgc ggaggctgtc cggcagggcg tgttcggcgg aatcgtattg ctcgacggtt
     2101 ttgtagtcga tgtcggagcc catgaagttg aaattagtcg tttcgccggt ggcggcctgg
     2161 ttggaaaaat tgcgcatggc gcgccggaat tttttcgatt tggcgatgtc caggctgccc
     2221 ccggcaaggg agtcggcata ggcggcggcg aggatactat cgagttcccg gttggaaaaa
     2281 agctgttttg ccgagtcccc gtaggccttt tttatcgagg tgatctcgat gttccggaat
     2341 tcgatccttt cgcggtaatc attcttgtcg tcctcgtcct cggcggaggc cagtctgttc
     2401 ctgagccggg ccgtgtcggc gcggaggtcg gcgagattgc gggtcttctg ctgctgctcg
     2461 gtcttgtcct cgcgcatggt cttagccatg tcctcgggtt tgcttatcgt gaagaggatg
     2521 ataaagaaga tggccgaggt gaagacatac atgcggatcg ggttcagata ggccatccgg
     2581 cggccgcgca tgtattcgat ggaaaggaag ccgggtttcc ggatcaggta gcgtaccgta
     2641 tcgaagaatt tcccgtcgaa gtgggtgatg tcgttgaaga agtgctggac gagatgccag
     2701 acggtctcct ttggttcgag gttctcctgg ccgcatttgt ggcagtagcg gccgatgacc
     2761 tcggtttggc agttaaggca gttcttttcc tttctttcct ttaagtgcga caaagcagtt
     2821 aaaattttaa ctaaaataag ggaaaggtcg gggtttcgtg tattaaattg attgtttttt
     2881 cgttacgacg gaggagattt aaatttgatc tatgaaagtt gtcgttggat gtttgttggg
     2941 gctggcgctt ggcggatcag tgcatgccca gtacctgtac aaagacctgg ttgtaacccg
     3001 gcagaataat gcccgctgga agctcttcga ggagaaccat gttaaatcgg tgaccctgaa
     3061 gagcctggaa gccgatggcc ggccgaccga gggttttttg ggcgaacagg aggtggcggg
     3121 tgactattcg cagatggtca cgcacaccca gacgtcgggc tcgacggatt cctggctcat
     3181 ttcccagtat gcgaacgggc ggatggtgaa gaacaccgat accagcgata cctaccagag
     3241 cgtgacggac tatacgtatg atacgggcgg tcacattatg tccattacta atacttctgt
     3301 cgaaacggat aaccatctgc gggatgtcga gcagcatatc tggagttatg accccgccca
     3361 ccccgggagg ccgttgtcga tgctgaagat caagaacggg accgatacca cccggatcac
     3421 cttcgtaacc gatgataagg ggaatatcgc cgaagagcgg agcacccggc taggggaggc
     3481 cctgccgacc atttactatt actataatga agacaacctt atgacggata tcgtccgtta
     3541 tagtatccgg gcgaagcggc tgctgccgct ggatatgttc gagtatgagg aagggcggct
     3601 gagatccatg cttgtcgtcc cggaggaggg gaacactttt taccagaaat ggtattatac
     3661 ctatgatgaa gagaaggggt taaagaccaa ggatatttgc tataacaagc aaaaggagct
     3721 gcttgggacc atcgaatacg tgtatagtta taaataaaag ttgccggagg ccggcacaca
     3781 taagccgccg gaggtggcat aaaatgaaca gtcatgcgtc gactcatttt tctttctctt
     3841 gtgctttgcg ggctttcggg gatggcccag gacctcgatt tccccgattt ccggaataag
     3901 aaggacaatt tctccaagat atcggacaag gatgtccggg cggatgtagc ctcgtttgcc
     3961 cttgcggggg tggatgaaag tatcgggaag gcgccgttga cggaggttcc cgttgtggac
     4021 ttcgggccga attttatcaa atacagcaac gacaatatcc aggtgacgat caaaacgggg
     4081 gtctttttcc cgtcgaagca caagctgatg atgcaggagg gacatctgtt gcggatagat
     4141 agtaaacctt attacggggg gatcatcgga cagccgccgc atacgacaat cgagtcgatc
     4201 acggtgttgg tggggaagga tacggtagcg ataccgccga cggccttttt cgatctgtat
     4261 gatccgaagt tcagctttaa ccagaatggg gcgacgagga gccgcaacgg ggtcttttta
     4321 tcgaacgaca agcatacctt ctatatctac cttctcaata tagataacaa aggtatcgag
     4381 tatacctttg ttatccgtga taagcaatac cttcgacgcg tggtcgactg gggctttttg
     4441 aattaagccg cgtcgacggt ttgtccctgc aatggctggc cggcgctttt tgccgcaagt
     4501 tgtttgtcga ctccatacat gaatatggcg cagatcataa atgccccaac ggtcacgaac
     4561 ccaagcgtgt cgtaatgcag cagtctcccg gttgcgtcct gggaaatgat aaacccgccc
     4621 atggcggagg cgatgccgcc gcccaactgc tggacagagg aattgatgct cataaaggcg
     4681 ccgcgatcgg gtccgtcggg tacggccgag atgagggcct ggcttgggat catccgggag
     4741 aaaacagccg tatacatcag ggtattgatc agcagcactt cccagagggg cgtgacggaa
     4801 agatgcgtga ggatctggac cattatggtg gcgaagatac tgccgtcgat gaacatgcgg
     4861 aacttgccga tgcggtcgct ccatttgccg atgagcggac cggtaacgag acctgccgcg
     4921 cctgctacga taaagacgat gggcaggatc ggctcgccga cccccacgtt gtgaacgagg
     4981 aaggtactgg agtagggcat gagcaggaag ccgccggtgg agagaaagac cgtgctgagg
     5041 aaggcgcgga gataacgctt ctgcgacacc gtgcgcagca ggtgggggaa cgggcgctgg
     5101 cgggcgcgtt gctgaaggtg atcggcaaca ggctgcatcc accggaggat gacaaggccg
     5161 accggcaggc agaatccgac gatcatgcgg aagggcgtgt gccagccgaa ccggttggcc
     5221 aggaaaagcc cgagggggat acccgcgacc tggctaacgg caaaggccat ctgtacatag
     5281 cccatcacg
//
LOCUS       DATGFG010000731         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1324171, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000731 DATGFG010000000
VERSION     DATGFG010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324171"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1258)
                     /locus_tag="VKQ52_13655"
     CDS             complement(110..1258)
                     /locus_tag="VKQ52_13655"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HLZ88293.1"
                     /translation="MKTAIFSYQSQEWRDHPGNDPLEKSKVSLVMALGNKAVLAPADF
                     YGQLRKEYPHAEIVLCSTAGEIFDDSVYEGSVSVTAVEFEKTKVRSAMVNIADFGGCS
                     YEAGKALVENLMAFDELAYILVLSDGSRVNGSDLVRGINSQVQDGVPVTGGLAGDGAA
                     FKSTLVGLNGVPDHGQIAAIGLYGKQLRVSHGSMGGWESFGPERTITRSTGNQLFMID
                     EESALDLYKKYLGPYAKELPGSALLFPLLVRRSNEEEPVVRTILSIDEGSKSMLFAGD
                     VPEGASVRFMKANFDRLVDAASAAANQTFTEHPREEPSLSVLISCVGRKIILGNRVTE
                     EVEAVKEAFGGKTLVTGFYSYGEIAPPFARVPCQLHNQTMTITNFSER"
     gene            complement(1546..2346)
                     /locus_tag="VKQ52_13660"
     CDS             complement(1546..2346)
                     /locus_tag="VKQ52_13660"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HLZ88294.1"
                     /translation="MPQATSIDEVLSQLEKIISDARRSGDRVGYFAALYHKVTSGVKA
                     GIARGDFEDGDRMARLDVLFANRYLQALEDWRAGLAVTDSWKIALEATKDPSLLVLQH
                     LLLGINAHINLDLGIAAVETIQNKQIGEIHRDFDAINAIIGSLTYQVLREIDRMSPFL
                     SLIGLHANRTDSFLVQFSIGNARDGAWCFAEDLSRMTGSAYSAFVMGRDNDVATLGRT
                     LADTTGLLRFTLWIIHLFEWHDPKKIIAELYGFTKRFMKAEEVRKGKR"
     gene            2556..2900
                     /locus_tag="VKQ52_13665"
     CDS             2556..2900
                     /locus_tag="VKQ52_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88295.1"
                     /translation="MKLTCICALLIAFSLTGAAQTTERYCMAEARFKGFSSVNATFIL
                     RPWTLDSLLTFQDSTILPRLSQVTRCTTIPDVLNYMASLGWTLAGVSGNTFFYFKRQF
                     KPSELRYPQPAN"
BASE COUNT          666 a         1032 c          695 g          729 t
ORIGIN      
        1 ttcaccgcct ctacgaaccg gatcattcgt tcatccgccc acaactccgg cggaagatgc
       61 ttcttgatct gtcgggtcaa tagtctgttc agtagtattg cctccggggt catcgttctg
      121 aaaaattggt gatggtcatc gtctggttat gcaactgaca gggaacccgg gcgaagggtg
      181 gagcgatctc cccgtaggaa taaaacccgg taaccagggt ctttccgcca aaagcctctt
      241 tgacggcttc cacctcttcg gtgaccctgt tgccgagaat gatcttccgg cccacacaac
      301 tgataagcac cgacagggat ggctcctccc gcggatgctc cgtaaaggtc tggttagccg
      361 cggcactggc cgcatccacc agccggtcga aattggcttt catgaaccgg acgctggcgc
      421 cctcgggcac gtcgccggca aaaagcatgc tcttcgatcc ttcgtcgatg gacaggatgg
      481 ttctcaccac cggctcttct tcattcgaac gccgaaccag cagcgggaat aacagcgccg
      541 accccgggag ctcttttgca taggggccca ggtatttttt gtacaggtca agagccgact
      601 cctcgtcgat cataaaaagc tggttgcccg tgctgcgggt gatggtcctc tccggtccaa
      661 aggactccca gccacccatg gacccgtggg aaacccggag ctgcttgcca tagaggccga
      721 tcgccgcaat ctgcccgtgg tcggggactc cgttaagccc caccagggtg cttttgaagg
      781 cagcgccgtc cccggcaaga ccgccggtaa cggggacgcc atcctgaacc tggctgttga
      841 tgcctctaac gaggtcgctc ccattgaccc ggctgccatc cgaaaggacc aggatatatg
      901 ccagctcgtc gaacgccatc aaattctcca ccaacgcctt tcccgcctca tagctgcaac
      961 ctccaaaatc ggcaatattc accatggccg accggacctt tgtcttttcg aactccaccg
     1021 ccgtgaccga caccgagccc tcatatacgc tatcatcgaa tatctccccc gccgtcgaac
     1081 aaaggacgat ctccgcatgg ggatactcct tgcggagctg cccgtaaaag tcggcaggcg
     1141 caagcacagc cttgttgcca agggccatta caaggctaac cttcgatttc tcgagtggat
     1201 cattcccggg atgatcccgc cattcctggc tttggtaaga aaagatcgcc gttttcatct
     1261 cgtgaattta ctccgccaac ggccgggaag aggtgatttc gataccatta caattatatt
     1321 cccccttttt aaagcgaatt ccccacttcc actacataca actacttaaa aacaccgcac
     1381 ctgcttcact gctccccgcc cgcatcccgt ctacatcccg ttctcatcat ctccccggct
     1441 tcctcgtctc ttctcctcct catctccacc tcgtcctcat ctcttcccct ccgccctctc
     1501 ccctccgtcc tcatcccaac ctcgctctct cgcccttcct cctccttatc tcttcccctt
     1561 cctcacctcc tccgccttca taaatcgttt cgtaaaccca tacaactccg cgatgatctt
     1621 cttcggatca tgccactcga aaagatgaat gatccacagc gtgaaccgca aaagaccggt
     1681 ggtatccgcc agcgtccgcc ccagggtcgc cacatcattg tcccgcccca tgacaaacgc
     1741 tgaatacgcc gaccccgtca tcctgctcag gtcctccgca aaacaccagg ccccgtcacg
     1801 ggcattcccg atactgaact gtacgagaaa cgaatccgtc ctgttcgcat ggagcccgat
     1861 cagcgacagg aacggcgaca tccggtcgat ctcgcgcaac acctggtagg tcagcgaacc
     1921 gatgatcgca tttatcgcat caaaatcccg gtggatctcc ccgatctgtt tgttctgtat
     1981 cgtctcgacg gcggcgatcc ccaggtccag gttgatatgc gcattgatgc ccaacagcaa
     2041 atgttgcagc accagcagcg acggatcctt tgtcgcctcc agcgcgatct tccaggaatc
     2101 ggtcaccgcg agacccgccc gccagtcttc caatgcctgc aaataccggt tggcaaaaag
     2161 cacatccagc cgcgccatcc ggtcgccgtc ctcaaaatcc cccctggcga tccccgcctt
     2221 cacacccgag gtaaccttat ggtaaagggc ggcaaaataa cctacccggt ctcccgacct
     2281 cctggcgtcg gaaatgatct tctccagttg cgataatact tcgtcgatgg atgtagcttg
     2341 aggcatacag cgattggatc taaggttagt agatgataat ggcgctgaaa atagctattt
     2401 gcgctttata tccatccgcg ccggtaaaaa tgggtgtttt tcccgtcgat gccccctcct
     2461 tttgcctact ccggcctggg ctcattgtta tacgttcgtt atagccggcg cttccccctc
     2521 ttctttgcgt accttgtaag gtaaaccttt ttcaaatgaa actcacctgt atttgcgccc
     2581 tcctcatcgc attttccctc acgggtgcgg cccaaacaac ggaaagatac tgtatggccg
     2641 aggcccggtt caaagggttc tcttcagtca atgcaacatt catcctgcga ccctggaccc
     2701 ttgattcctt attgactttt caggacagca ccatcctgcc ccggctcagt caggtcaccc
     2761 gatgcacgac catccccgat gtcctcaatt atatggcttc ccttggatgg acactcgccg
     2821 gcgtaagcgg taataccttt ttttatttca agcgacaatt caagccctcc gaactgcgct
     2881 acccacagcc cgcaaactaa catggatcaa tattatcgtt acaggttggg cgttccccgc
     2941 cgccgcaaac caatgcgaag cggcgggtca ggctcttcgc tccaagtcct cgccttcggc
     3001 tccgggcttt tcacttcgat ccttaacgct tcgtgaaaga acttctacaa acgacgattc
     3061 tcggaacttc ccaaatcgaa ttgaagcgag aatggatatc cttatccatc ggctgacttc
     3121 tc
//
LOCUS       DATGFG010000732         5126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1506285, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000732 DATGFG010000000
VERSION     DATGFG010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506285"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /locus_tag="VKQ52_13670"
     CDS             complement(<1..1844)
                     /locus_tag="VKQ52_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLZ88296.1"
                     /translation="MADNKVEAIVAKARGNGIFFVLEGDVLRLRVPKGAVTDPELIRL
                     IQEEKEGIKALLLLEARAGSSPNGRRIMGGISRDWQGRLPLSFSQERLWFIHQLQGSI
                     QYHEPSVWRLGGVLHVDSLQLALQEIVNRHEVLRTVIGEEGGQPYQRVLDKGGWSLGI
                     FGGGGDTPDTAALRDRVEALIRAPFDLAVDHPLRAELIRAGEEEHLLVVTLHHIASDG
                     WSMAILVAELVELYGAYVEGRAPQLEPLDLQYADFALWQRNYLDTEVLPSKLGYWKAQ
                     LAGAEPLQLPTDFGRPAVQSMRGASFLSGIDEGLTESLRRLALQEGATLYMVLLAAFQ
                     VLLHRYSGQEDICVGSPVAGRQQQEIEGLIGFFVNTLVIRGQVSDGMSFRELLGQVKG
                     TTLGAYGHQDVPFEKVVEAVMNQRDQSRNPLFQVLFGLQNIPELPALEFGAVRWRRES
                     VVNNTSKFDLSVMLQEGRGGLMMDIGYCTDLFTEDTVRRMADHYRELLQSVVADADRQ
                     VGKLDLVGGAERRQLLLDFNDTTVMYPRDRTVVSLLEEQAQRTPDAVALVFEEESLTY
                     RELDERANQLGHCLRSRGVGRDVLVPLCVQRSMEMVIGILGIWKAGGA"
     gene            complement(1877..3493)
                     /locus_tag="VKQ52_13675"
     CDS             complement(1877..3493)
                     /locus_tag="VKQ52_13675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF037824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLZ88297.1"
                     /translation="MDERIVFLKPNVVIEPLVDRWYAWSHLISPATAAMNILGRHMTI
                     MQSYLSDPGIHAEAVLNPKMRGGPFMDLGGGQVEAVKALYDNTVEKRATLIQFAQAVK
                     ELDRILLTEAKGYSLEQMYKRVPEILRGFVELYYDRNNCATFRLFESLLYRSQFYRKD
                     SQSIALWVTTNDHRPFSLSTPRLDAADVLHLPMPFDHAGIDELSKMKRNAQPLGYIKE
                     VMGITPEQERLFETFFTEDPPPKYKKYTGDRIRMRYFGHACILVETKDVSILMDPLIS
                     YYGYYSDLEHFSDADLPDVIDYAMITHNHQDHILFETLLPLRHKIRNLVVPRTYGGRL
                     EDPDLAMMFRNIGFKNVFSIEEMETVHFGDVDITGLPFVGEHADLNILTKSCYLVQSG
                     DFKLLFLADSRIVDADVYKHIHRQIGNVDVLFLGMECDGAPMSWLYGPLMSKKLMRDQ
                     DNSRRLAGSDFAMGMNVVDIFHPKEAYVYAMGQEPWIEFITSIKYTDQSNPIVQSNML
                     VKRCGELGIISERLYGEKEILYDKHVVAVE"
     gene            complement(3505..4212)
                     /locus_tag="VKQ52_13680"
     CDS             complement(3505..4212)
                     /locus_tag="VKQ52_13680"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLZ88298.1"
                     /translation="MKINVLSLPFAGGNRHSFRAFQRQSPDYIKFVVLEYPGRGERTH
                     EGLIRNIHRLVEDLFRQAKKHIAQGRYALYGHSLGGLATWLLARKIVENHLPPPVHLF
                     ITGAIGPGSDVRMEDNKRHLLGKGDFLQMMKDLQGMPDEMFRDSELLAYYEPILRADI
                     QVSESYVHRELEPLDIPFTVISGAGEEGEPADYQIWQKETKRKVCFYQLPGGHFFILD
                     HAREVVEIILKELSFYL"
     gene            complement(4202..>5126)
                     /locus_tag="VKQ52_13685"
     CDS             complement(4202..>5126)
                     /locus_tag="VKQ52_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ88299.1"
                     /translation="LTGVYCWYLVLGVVMFVIPAAFEISVAQMAAFITSILFIMSPVS
                     QLLSFFPAYNTYKIAFERVALIDEQLSSGNLHPQFEAPSKKHFESIRFEQIVYRYGLD
                     DGSTFTLDLAELRLNKEEIVFVTGGNGSGKTTFINILTGLCKPLSGKVFINEEEVSWE
                     EFAHFNNNMAVVFADYYPFKENYNDYDLFDTGGRCEELINRLHLRGNVELDWENRKLH
                     TNLSRGQEKRMALLFALLEDKPILILDEWAAEQDQHNRECFYHEWIADIRRSGKTVII
                     ISHDEAFYDAADRVVRFHYGKIMTNAEPVEN"
BASE COUNT         1245 a         1590 c         1109 g         1182 t
ORIGIN      
        1 tacgcgcctc ccgccttcca tatccccaat atcccgatga ccatctccat gctccgctgc
       61 acgcagaggg gcacaaggac atcccggcct acaccccgtg accgcagaca atgccccaac
      121 tggttcgccc gctcgtcgag ctcccggtag gttagcgact cctcttcaaa gacgagagcc
      181 actgcatccg gcgtccgctg cgcctgttct tccaacagtg acaccaccgt cctgtcccgc
      241 ggatacatca ccgtcgtatc attgaaatcc aatagcagct gccggcgctc tgcaccgcca
      301 accaggtcga gcttcccaac ctgcctgtca gcatcggcca ccaccgactg caacagctcc
      361 cggtaatggt ccgccatccg cctgacagta tcttccgtga acagatctgt acaatacccg
      421 atgtccatca tcagcccacc gcgaccctct tgcagcatta cggacaagtc aaattttgaa
      481 gtgttgttta caaccgactc tctgcgccac ctgacggcgc caaattcaag cgcgggaagc
      541 tcaggaatgt tttgcagccc gaacagcacc tggaacagcg ggttcctgct ttgatcgcgt
      601 tggttcatca ccgcctctac tactttttcg aagggcacat cctggtggcc gtaggcgccc
      661 agcgtcgtcc ccttcacctg acccaggagt tcccggaatg acattccatc actcacctgc
      721 ccccttatca ccagcgtgtt cacaaaaaaa ccgatcaacc cctctatttc ctgctgctga
      781 cggcccgcca ccggactgcc cacgcagata tcttcctgtc cgctgtaccg gtgcagcaac
      841 acctgaaaag cggcaagcaa caccatgtac aaggtcgccc cttcctgcag cgccaatcgc
      901 cgcagggact ccgtcagccc ttcgtcgatg ccggataaaa agctggcgcc ccgcatgctc
      961 tgtaccgccg gccgtccaaa gtcggtcggc aactgcaacg gctccgcgcc cgccagctga
     1021 gccttccaat agcccagctt cgatggcaat acttccgtgt cgaggtaatt ccgctgccag
     1081 agggcaaaat ccgcgtactg cagatcgagc ggctccagct gcggggcacg gccctccaca
     1141 taagccccat atagttccac gagctccgcc acgaggatcg ccatcgacca cccgtccgaa
     1201 gcgatatgat gcaaggttac cacaagcaaa tgctcctctt ctcccgcccg gatcagctcc
     1261 gcccgcagcg gatgatccac agccaggtcg aatggcgccc ggattaatgc ctccacccta
     1321 tcccgcaatg ccgccgtatc cggcgtatcc ccgcccccac cgaagatgcc taaactccat
     1381 ccccccttat ccagcacccg ctgatagggc tgccctcctt cctcgccgat taccgtgcgt
     1441 aacacctcgt gccggttgac gatctcctgc aacgccagct gcaggctatc gacatgtaac
     1501 acacccccta atcgccatac cgacggctca tgatactgaa tactaccctg cagctggtgg
     1561 ataaaccaca accgctcctg gctaaaagac aatggcagcc gtccctgcca gtcccgggat
     1621 atcccaccca tgatacgtct tccgttcggg gaagatcccg cccgggcctc aagcaacagc
     1681 aaggctttga taccttcctt ctcctcctgt atgagcctta tcagctcagg gtcggtgaca
     1741 gctcctttgg gcactctcaa tcgcagaaca tcgccctcca atacaaaaaa gatcccattg
     1801 ccgcgagcct tcgcgacaat ggcttcaact ttattatcag ccataaaagt tattccatta
     1861 aattcttact gaaatttcac tccacagcaa caacatgctt gtcatacaga atttccttct
     1921 ctccatacaa ccgctcggaa atgatcccca gctccccaca acgtttcacc agcatattgg
     1981 attgaacaat ggggttggat tgatccgtat attttatgct cgtgataaat tctatccacg
     2041 gctcctgccc catggcatag acataagcct ccttcggatg gaatatatcg acgacattca
     2101 tacccatcgc aaaatcagac cccgcaagcc ggcggctgtt atcctgatcg cgcatcagct
     2161 tcttgctcat caacggacca tacagccagg acatgggagc tccgtcacat tccattccca
     2221 ggaaaaggac atctacattc ccgatctgcc tgtgtatatg tttatagaca tcggcgtcca
     2281 cgattctcga atcggccagg aaaagcaatt taaaatcacc actttgcacg aggtagcaag
     2341 acttggtcag gatattcaga tcggcatgct cacccacaaa cggaagaccg gtgatatcca
     2401 catcgccaaa atgcaccgtt tccatctcct ctatcgaaaa aacgttctta aagccgatgt
     2461 tccggaacat cattgccagg tcgggatcct ccaaccggcc gccataggtc ctcggtacaa
     2521 cgagattcct gatcttgtgc cgcagcggaa gcaaggtctc gaagaggata tgatcctggt
     2581 gattatgggt gatcatggcg taatctatga catcggggag atcggcatcc gagaaatgtt
     2641 ccagatcgct atagtagcca taatagctga tcagcggatc cataaggata gaaacatcct
     2701 ttgtctccac caggatacag gcatgtccga aatagcgcat ccgtatccgg tcgcccgtat
     2761 atttcttgta cttcggcgga ggatcttcgg taaaaaaagt ttcgaacagc cgctcctgct
     2821 ccggggtgat ccccatcacc tctttaatgt atcccaacgg ctgcgcgttt cgtttcattt
     2881 tcgacagctc gtctatcccc gcatgatcaa aaggcatagg cagatgcaac acatccgccg
     2941 catcaagccg gggagtggac agcgaaaacg gccggtgatc gttggtcgtg acccaaagcg
     3001 caatgctctg ggaatccttc ctgtaaaact gactccggta gagaagtgac tcgaaaagcc
     3061 tgaatgtggc acagttattc cgatcgtaat agagttccac aaagcccctg agtatctcgg
     3121 gaacccgttt atacatctgt tccaggctat agccctttgc ctccgtcaaa agtatccggt
     3181 cgagctcctt gacggcctgc gcaaactgga tcagcgtcgc ccgtttctca acggtgttat
     3241 cgtacaacgc ttttaccgct tccacctgtc ccccgcccag atccataaag ggtccgcccc
     3301 tcatcttggg attcaacacg gcctccgcat gaatgccggg atccgagaga taagactgca
     3361 tgatcgtcat atgcctcccc agtatattca tcgccgctgt agccggcgag atcaggtgac
     3421 tccaggcata ccatctatcg accaaaggtt cgataaccac gttaggtttc agaaagacaa
     3481 ttcgttcgtc catattcttt gtttttataa ataaaaagac aattccttca ggatgatctc
     3541 cacaacctca cgggcatgat caagtatgaa aaaatgcccc cccggtagct gatagaagca
     3601 gaccttgcgc ttcgtttctt tctgccatat ctgataatca gccggctcac cttcttcccc
     3661 ggccccggag attaccgtaa agggaatatc cagcggttcg agctcccggt gtacatacga
     3721 ctcgcttacc tggatatccg cccgcaggat gggttcataa taagccagca gttcgctatc
     3781 cctgaacatt tcatccggca tgccctgaag atccttcatc atttgcaaaa aatccccctt
     3841 acccaggaga tggcgcttgt tatcttccat cctgacatcc gaaccgggtc ctatcgcgcc
     3901 tgtaatgaaa agatgcaccg gaggcggcaa atgattttca acgatctttc tcgccagaag
     3961 ccaggttgcc agcccgccca ggctatgccc gtaaagagca tatcttccct gcgcgatatg
     4021 cttcttcgcc tggcgaaaaa ggtcttcaac caatcgatgg atatttctta tcaacccttc
     4081 atgggtccgc tcgcccctac ccgggtattc caggacaacg aatttgatat agtcagggga
     4141 ctgtcgttga aaagcacgga aagaatgcct gttacctccg gcaaaaggca gggaaagcac
     4201 attaattttc aaccggctcg gcatttgtca tgattttgcc ataatggaac ctcacgaccc
     4261 tgtcagccgc atcatagaac gcttcatcgt gggatatgat gatcaccgtt ttacccgaac
     4321 gtctgatatc agcgatccac tcatgataaa aacattcgcg gttatgttga tcctgctcgg
     4381 cagcccattc atccaggata aggatcggct tatcttccag cagcgcgaat agcagcgcca
     4441 tccttttttc ctgtccccgg gaaaggttcg tatgcaattt cctgttctcc cagtccaact
     4501 cgacatttcc cctgagatgc agccggttga tcagctcttc gcatcttccg cccgtatcga
     4561 aaaggtcgta atcattataa ttctccttaa aaggataata gtccgcaaag accacagcca
     4621 tattgttgtt aaaatgcgca aactcttccc agctgacctc ctcttcattt ataaacacct
     4681 ttccactcag cggcttgcac aggcccgtca atatattgat aaacgtcgtt ttcccactcc
     4741 cgtttcctcc ggtgacaaac acgatctcct ctttgttcaa ccttagctcg gccaggtcga
     4801 gcgtgaacgt agacccgtca tccagaccgt atctatagac tatttgttcg aaccttatcg
     4861 actcgaagtg cttttttgaa ggcgcctcga attgcggatg caagttcccg ctgctcaatt
     4921 gctcatcgat gagcgcgacc cgttcgaaag cgatcttata ggtattatag gccggaaaga
     4981 aggaaagcag ttgcgagacg ggagacatga taaatagtat agatgtaata aaagccgcca
     5041 tttgtgctac actgatctca aaagcagccg ggatcacgaa cattaccact cccagcacca
     5101 gataccagca gtaaacaccg gttaat
//
LOCUS       DATGFG010000733         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_303530, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000733 DATGFG010000000
VERSION     DATGFG010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303530"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..2214
                     /locus_tag="VKQ52_13690"
     CDS             175..2214
                     /locus_tag="VKQ52_13690"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88300.1"
                     /translation="MKYSKQLSTNARIIKDLLTKYKDTFVAFCELLNNAIQAKATRIE
                     LTIDYANSATARAPFTRIELVDNGIGVNATDFEKKILEIGTDVKKNGQGIGRFGALQL
                     GEKMTITTVAEDGERGCMTQVVFPLDTGSISSSLSKVNLEFDVEDLKKKVNPYYRVVI
                     ENLHHNKQGRLLPKNKIADKFRQESIEQALFEKYPLQVFNRELRFVVNGKELDPAGFV
                     VDRPHIKTVVYTDIHQNGHSFDFHFYQLRIDVGKVKVFFYVDNAGIKTVAHEFTYTSD
                     WYTPDLGTWFIYLDSDFFDTGLFRNIDLDSLGDEEIRRLKDFIKDTINDFFRAQNRRF
                     EKFLADLEKDPSYPASFESPISASRELLFQKIAYLVEDEYKLLGKDEQLRGLVYSLID
                     RSLASGHVEEVFSKVVRLSDTNMERFHQLLEKTELENIITFASAVANKLEFLEFLHEL
                     IYGDVGAVLRERSQLHKIIERELWLFGEAYNGTPHLWSDKKIGNILKELRDRFLVYEP
                     SAGDENLIGGVEGLDNITDLFFFNEKILDNEDREVMVVELKSPRCAIGKKELQQIDEY
                     AYTVESFPGLPKENIHYTFVLISSKLTAYAKSKMKSARGKYHQPFLYDRKSEKNIDVF
                     VMEWSELMEVNKRKLGYLSAKLKVKDKSVRSTFETEYPGIINEKVNARLTRTKGR"
BASE COUNT          542 a          441 c          803 g          572 t
ORIGIN      
        1 gcgcggtgag tggccggcgg aggcgcggtg agatgccggc ggggggttga tttgcgggag
       61 gtgattggcg gagggttgat tttgctggat gcttttggcg aggtgctgcg atactaaaaa
      121 atatggtatt tttggagcct atattgccaa tatttttagt atatttgagg ggttatgaag
      181 tacagcaaac agctttcgac gaatgcgcgg atcattaagg acctgcttac caagtataag
      241 gacacgttcg tggccttttg cgagttgctg aataatgcga tccaggcgaa ggcgacgagg
      301 atcgagctga cgatcgacta tgcgaattct gccacggcgc gggcgccctt tacgcggata
      361 gagcttgtgg ataacggcat cggcgtgaat gcgaccgatt tcgagaagaa gatcctggag
      421 atcgggaccg atgtgaagaa gaacgggcag ggcatcggcc ggttcggcgc gttgcagctc
      481 ggggagaaga tgaccattac cacggtggcg gaggatgggg agcgcgggtg tatgacccag
      541 gtggtgttcc cactggatac cgggtcgatc tcatcctcgc tttcgaaggt gaacctggaa
      601 ttcgatgtgg aggatttgaa gaagaaggtg aacccgtatt acagggtagt gatcgagaac
      661 ctgcatcaca acaaacaggg gagattgttg ccgaagaata agatcgccga taaattcagg
      721 caggagagca tcgaacaggc gttgttcgag aagtacccgc tgcaggtgtt caacagggaa
      781 ctgcgatttg tcgtcaatgg taaggagctg gacccggcgg gttttgtggt ggaccggccg
      841 catatcaaga cggtggtata tacagatatc caccagaacg ggcactcgtt cgatttccat
      901 ttttatcagt tacggatcga tgtcggcaag gtgaaggtgt tcttttatgt ggacaatgcg
      961 ggcatcaaga cggtggcgca tgaattcacc tatacctcgg actggtatac acccgatctg
     1021 gggacctggt ttatttatct ggattcggat ttctttgaca cggggttgtt ccgcaatatc
     1081 gatctggatt cgcttgggga tgaggagatc cggcggctga aggattttat caaggatact
     1141 ataaatgatt tcttccgggc gcagaacagg cgattcgaga agtttcttgc cgatctggag
     1201 aaggatccct cctaccctgc ttcgttcgag tcgccgatct cggcgtcgcg ggagctgctt
     1261 ttccagaaga tcgcgtatct cgtggaggat gagtataagc tgctcggcaa ggatgagcag
     1321 ctgcgggggc ttgtttattc gcttatcgac cgatcgctcg caagcgggca tgtggaggag
     1381 gtctttagca aggtggtgcg gctatcggat acgaatatgg agagattcca ccagttgctg
     1441 gagaagacgg agttggagaa tatcatcacg tttgcgagcg ctgtggccaa taaactggaa
     1501 tttcttgagt tcctgcatga gctgatctat ggggatgtgg gggcggtgtt gcgggaaagg
     1561 agccagctgc ataagatcat cgagcgggag ctatggcttt ttggggaggc gtacaatggg
     1621 acgccgcatt tgtggagtga taagaagatc gggaatatcc tgaaggagtt gagggatcgg
     1681 ttccttgtct atgagccgag tgccggggat gaaaacctga tcgggggggt ggaggggttg
     1741 gataatatca ccgatctgtt tttcttcaat gagaagatcc tggacaatga ggaccgggag
     1801 gtgatggtgg tggagttgaa gtcgccgcgg tgtgcgatcg ggaagaagga gttgcagcag
     1861 atcgatgagt atgcgtatac ggtggagtcg tttccggggt taccgaagga gaatatccat
     1921 tatacctttg tgctgatctc atcgaagctg acggcctatg cgaagtcgaa gatgaaatcg
     1981 gcccggggga agtatcatca gccttttctc tatgaccgga agtcggagaa gaatatcgat
     2041 gtttttgtga tggagtggtc ggagctgatg gaggtgaaca agcggaagct ggggtatttg
     2101 tcggcgaagt tgaaggtgaa ggataagagt gtacggtcga ccttcgagac ggagtatccg
     2161 gggatcatca atgagaaggt gaatgcgcgg ttgacgcgga cgaaggggag atagggggtg
     2221 agcatgggtt attccagata gaggagggcg aattgttgtc cggtggtggt gctgttgttg
     2281 gcgatgtcca ggctgtgatc gccggggacg aagaggtagt cgcctttttt ggtgaaggga
     2341 tgtttgttga ccatcccg
//
LOCUS       DATGFG010000734         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_380357, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000734 DATGFG010000000
VERSION     DATGFG010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380357"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VKQ52_13695"
     CDS             complement(<1..664)
                     /locus_tag="VKQ52_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLZ88301.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEMTNTISARDMQAQVLDDM
                     DLEREKGITIKSHAIQINYASKGQQYILNLIDTPGHVDFSYEVSRALAACEGALLLVD
                     ATQGIQAQTISNLYLAIENDLEIIPVINKVDMDGAMVDEVKDQIIELIGCKPEDILLA
                     SAKTGLGVPEILDAIVDRIPAPKGDPDAPLQALIFDSVFNSFRGIIVYYRILNGTLKK
                     GDK"
     gene            705..1322
                     /locus_tag="VKQ52_13700"
     CDS             705..1322
                     /locus_tag="VKQ52_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88302.1"
                     /translation="MKPSQTNFDKAEIARRGSGRPLKCEAFTETIEKTSWFRGKRHKI
                     RANYWLIGFFILQIGIDLAHSVTVFPFVHYGMFSASVARPDSVEVFRVSVDGRLLQPG
                     DLAIYRWDMVGTPLEAYERRLATGDYAFDKEKLQAGMARAGLSSLYNQLKSNLYNTGS
                     FIPWYKGYLGRLLGQPIGILRVDKAWYRWAGGGLQLVRTENWING"
     gene            1315..>2317
                     /locus_tag="VKQ52_13705"
     CDS             1315..>2317
                     /locus_tag="VKQ52_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88303.1"
                     /translation="MGNWESRRYARDRRLLLTVYFACYFAVFLFLAIDHRLLAQVRPV
                     VFNFNRDLTELALIATGLPRWMIAHPWSFWLADAGLFLLPAFLLSYAIGKGRMPPGLV
                     VAFLLFLSLYLLLADIFWQVHQEPFIGYVLLPLAFLIGRADRFYRILQACRYYFLYVF
                     VSAAIWKIARGAVFNSDEMSRILLVHHGDLLSGDCTGTACRAYTWLIGHPFAAQLLYL
                     GGVGLEACFLAGFFTRRFDRWLLGLAVLFVVADLLLMRIPYWTILLGGITLWIDPRPR
                     QPVMVVYETTHHENLPALLDLCETRFPRVVVFLKELSYQNISGTGSLASRWPRTAFIV
                     "
BASE COUNT          421 a          647 c          655 g          594 t
ORIGIN      
        1 ccttatcccc ctttttcagc gttccgttaa gtatccgata atacacgatg atcccccgga
       61 aggaattgaa tacgctatcg aagatcaggg cctgcagcgg ggcatccgga tcgcctttag
      121 gggcgggaat acggtccacg atggcgtcga ggatctccgg aacgccgaga ccggtcttgg
      181 cgctggccag caaaatgtcc tcgggtttgc agccgataag ctcgatgatt tgatccttta
      241 cttcatccac catcgccccg tccatatcaa ctttattgat aacggggatg atctcaagat
      301 cgttttcaat ggcaaggtag aggttggaga tggtctgcgc ctggataccc tgggtcgcat
      361 ccaccagcaa cagggcgcct tcgcaggcgg cgagggcccg gcttacctcg tagctgaagt
      421 cgacgtgacc gggcgtatca atcagattaa gaatatattg ttgtcctttt gaagcatagt
      481 tgatctgtat cgcgtggctc ttgatggtaa tccctttctc ccgttccagg tccatatcgt
      541 ccaatacctg ggcttgcatg tcccgggcgg agatggtatt ggtcatctcc aaaagccggt
      601 ctgcaagagt actctttccg tggtcgatgt gggcaattat gcaaaaattc cgtatatttt
      661 tcattcgctg caaatctacg caaataggtc cataaattag gggtatgaaa ccgagccaga
      721 cgaattttga caaagctgaa attgcccggc gaggttccgg ccggccattg aaatgcgaag
      781 cattcaccga gaccattgaa aagacatctt ggtttcgagg taaaagacat aaaatccggg
      841 cgaactattg gctcatcggc ttttttatcc tgcagatcgg catcgatctg gcccattcgg
      901 tcactgtttt tccctttgtt cactatggga tgttctcggc atcggttgcc cggcccgaca
      961 gcgtcgaagt gttccgcgta agcgtggacg gcaggctgct ccaaccgggc gatcttgcta
     1021 tttaccgctg ggatatggta ggaacgccgc tggaggccta tgaacgacgc ctggcgacgg
     1081 gcgactatgc cttcgataag gaaaaattgc aggcgggcat ggcgcgggcg ggtctttcct
     1141 ctctttataa tcaattgaaa tccaacttat acaatacggg ctcctttatt ccctggtaca
     1201 agggctacct gggccgtctc cttgggcaac ccattggcat cctgcgggtg gacaaggcct
     1261 ggtatcgctg ggccggcggg ggcctgcaat tggtgcgaac cgaaaactgg atcaatgggt
     1321 aattgggaaa gcaggcgcta tgcccgcgac aggcggctgt tgctgaccgt ctattttgcc
     1381 tgctattttg ccgtctttct tttcctggcg atcgatcacc ggctactggc gcaggtgcgg
     1441 cccgtggttt tcaacttcaa tcgcgacctg acggaactgg cgcttatcgc gacgggtctg
     1501 ccgcgatgga tgatcgccca cccctggagc ttttggctgg ccgatgcagg gctgttcctg
     1561 ctgcccgcct tcctcctgtc ctatgccatt gggaaaggaa ggatgccgcc gggcctcgtc
     1621 gtggcgttcc tcctcttcct ttcgctctac ctgctgctgg ccgacatctt ctggcaggtg
     1681 caccaggagc cgttcatcgg ctatgtgttg ctcccccttg cctttctcat cggtcgggcg
     1741 gaccggtttt atcgcatcct gcaggcatgc cgctactatt tcctgtatgt ttttgtgtcg
     1801 gccgctatct ggaagatcgc caggggcgcc gtcttcaata gcgatgaaat gagccgtatc
     1861 ctgctggtgc atcatggcga cctgcttagc ggtgattgta ccgggacggc ctgccgggcc
     1921 tatacctggc tgatcggtca tccgtttgcc gcccagctgc tctatctcgg tggcgtgggg
     1981 ttggaagcct gctttctcgc cggctttttc acgcgccgct tcgaccggtg gttgctgggg
     2041 ctggcggtcc tttttgtcgt ggccgatctg ctgttgatgc gcatccccta ctggacgatc
     2101 ctgcttggcg ggatcaccct ctggatcgac ccccgcccgc ggcagccggt gatggttgtt
     2161 tatgagacga cccaccatga gaacctgccg gcgctgctcg acctctgtga gacgcgtttt
     2221 ccgcgggtgg tcgtcttcct caaggaactc tcctatcaaa atatctcggg tacgggcagc
     2281 ctggcttccc gctggccgcg aacggcgttc atcgtcc
//
LOCUS       DATGFG010000735         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1010440, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000735 DATGFG010000000
VERSION     DATGFG010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010440"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VKQ52_13710"
     CDS             <1..1066
                     /locus_tag="VKQ52_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88304.1"
                     /translation="TTSSADLATHADRGVSRPLDLLFVQGSPRSIDLSFVQGVDGTLS
                     MIYGSAANYTLVRIGIDGKPNAFPLSGLPDGNFRYVSWVAEGETLRFSGLLSRTRKTN
                     FTAIVTGSVDLTTGTVFNLRQTEVATLMAQAPPALRDRVQNGFPADLTLLRTLTLSDA
                     SRVVLFESSGQHLYQNHFSPATQNPISAFGRSGTFTSSSPSTEGIRYQSRGDVYVLKL
                     DRDNVPQWLNVLSKNQEASEKTTAIGIGCLADQQDNLHIFFYDSKYNADAYAANPTPV
                     RTDDPRGNEFACISITPDGAMKKQFIPITDQHYRLMPELAFVDNKNEACFLAVKTKMS
                     ITEQLASDNNGYKLGTIAVK"
     gene            complement(1278..1913)
                     /locus_tag="VKQ52_13715"
     CDS             complement(1278..1913)
                     /locus_tag="VKQ52_13715"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLZ88305.1"
                     /translation="MSLRLGDKAPNFQAKTSIGDIDFYEFLGDSWGILFSHPADYTPV
                     CTIELGRTAGLKDEFAKRGVKVLALSVDPVDKHIGWIKDINETQHCTVDFPIIADEDR
                     KVSTLYEFIHPNASATATVRSLVIIGPDKLVKLIITYPASTGRNFNEVLRVVDSLQLT
                     ANHSVATPADWKEGEDVIVVPSISTEDAQKKFPKGVKVIKPYLRYTPQPNK"
     gene            complement(2348..2848)
                     /locus_tag="VKQ52_13720"
     CDS             complement(2348..2848)
                     /locus_tag="VKQ52_13720"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ88306.1"
                     /translation="MKITAAYTREQFETMEALGRRIVPDFYAQYLPPECGDYLVRASH
                     TIEAFESQVGEGYRHYLVEIDDTIVGYFALHEEEDRTMVLTQFYLLKEWRGRGLGQRV
                     MDFIHREAAELRVKKIELLVFRKNEGAVGLYRKNGYIVAAEVLTPMGSGHAVEDYIMQ
                     KRFDRQ"
BASE COUNT          629 a          946 c          761 g          618 t
ORIGIN      
        1 gacgacctcg tcagccgacc tcgccaccca cgcggatcgc ggcgtctccc gtcccctcga
       61 tctcttgttc gtccagggat cacctcgctc catcgatctt tccttcgtcc agggagtcga
      121 cggaaccctt tcgatgatct acggctcggc cgccaactat accctcgtac gcataggcat
      181 cgacggtaaa ccaaacgcat ttcccctgtc cggcctgccc gatgggaatt ttcgctatgt
      241 ctcctgggtg gccgaaggcg agacccttcg cttttccgga ctgctgagca ggaccaggaa
      301 aaccaacttc acggcgatcg tcaccggaag cgtcgacctc acgactggca ccgttttcaa
      361 cctccggcag accgaagtcg ccacgctcat ggcccaggcc ccgccggccc tgagagatcg
      421 ggtgcagaac ggatttcccg ccgacctcac cctcctgcgt acgctgaccc tgagcgatgc
      481 ctcccgggtc gtactcttcg aaagcagcgg gcaacacctg tatcaaaatc atttttcccc
      541 ggcgacgcaa aaccccattt ccgcctttgg ccggtcgggg accttcacct ccagcagccc
      601 ctcgacagag ggcatccgct atcaaagccg cggggacgta tatgtgttga aactggacag
      661 ggataatgtg ccgcagtggc tgaacgtgct gtccaagaac caggaagctt cggagaagac
      721 caccgccatc ggcatcggct gcctcgccga ccagcaggac aacctgcata tcttcttcta
      781 tgacagcaag tacaatgcgg atgcctacgc cgccaatccc acgcccgtcc gcaccgacga
      841 cccgcggggg aacgaattcg catgcatctc cattaccccc gacggcgcca tgaaaaaaca
      901 attcatcccc atcaccgacc agcactaccg gctcatgccc gaactcgcgt tcgtcgataa
      961 caagaacgaa gcctgcttcc tcgccgtaaa aacaaagatg agcatcaccg agcaactcgc
     1021 ctccgacaac aacggctaca aactgggcac catcgcggtc aaataggcgg ccgcgagctc
     1081 cacgacccgg ccccggcacc cggccccggc ccaaaacaaa aagggccaac cgtccatcgg
     1141 ttgaccccat atcgaaacct attcaattac tacttattcg gctgcggcgt gggttgctgt
     1201 cgtcggctaa tctgaaaagt aagcctcctc ccgcaacggc taacgattgg ccttcggccg
     1261 acacgccgct atccgcctca cttattcggc tgcggcgtgt atcgcaggta aggcttgatc
     1321 accttcactc ctttcgggaa cttcttctgc gcatcttccg tgctgatcga cggaacgacg
     1381 atcacatcct caccctcttt ccaatcggcg ggcgtagcca cgctatgatt ggccgtcaac
     1441 tgcagggagt cgacaacccg gaggacttcg ttgaaattac gaccggtgga agccgggtag
     1501 gtaatgatca gcttcaccag cttatccgga ccgataatga cgagcgaacg aaccgtagcc
     1561 gttgccgaag cattcggatg gatgaactca tacagggtgc tcaccttgcg atcttcatcg
     1621 gcgatgatcg ggaagtcgac ggtgcaatgc tgcgtctcgt tgatatcctt gatccagccg
     1681 atgtgcttgt cgactgggtc cacactcagc gccagcacct tcacacctct cttggcaaac
     1741 tcgtctttca gaccggccgt ccggcccagt tcgatcgtac acacgggagt ataatcggca
     1801 ggatgggaaa agaggattcc ccaggaatca cccagaaatt cgtagaagtc gatgtcgcca
     1861 atcgatgttt tggcctggaa gttgggggcc ttatccccga gacgtagaga cataaaattc
     1921 aaaattttgg tttaaaaaat ctgttgatgc cgggtattgc ccggcttcat cttggtttaa
     1981 gaatcgtcga agttgggtat tgcccggctt cattttggtt taagatccgt cgatgccggg
     2041 tgaccccggc ttcctaacgg ggggtaaaga tattaattcc ctatcaaaat agtagactta
     2101 aatgttaatt tttaatttcg gccgggagcg cgagggggcc gcggcggtgg cgggggtgtg
     2161 gccggcggcg gtgccggggg catgatggcg gcggtgccgg cagtgccgtg ggggcgtggg
     2221 tgtgcagcgg cgggggtgag gcggttcggg aggcgtggca cagcggccga gggagtggcg
     2281 gcagccacac aacttattgt taatcaatcg aatatgcatt tactgtccat caaacccctt
     2341 ctgcatttta ctgtctatca aaccgcttct gcattatata gtcctctaca gcatggccgc
     2401 tgcccatggg ggtcaacacc tccgccgcaa cgatatagcc gttcttccga tacaggccca
     2461 ccgccccctc attcttccga aacacgagca gctcgatctt ctttacccgc aactcggcgg
     2521 cctcgcggtg gataaaatcc atcacccgct gccccagtcc ccggccgcgc cattccttca
     2581 gcaggtagaa ctgggtcagc accatcgtac gatcctcctc ctcatggagc gcaaaatacc
     2641 cgacgatggt gtcatcgatc tccaccaaat aatgccggta cccctcgccc acctgcgact
     2701 cgaatgcctc gatggtatgc gatgcccgga caagataatc cccgcactcc ggcggtaaat
     2761 actgggcgta aaagtccggc acgatccgcc ggcccaacgc ctccatcgtc tcgaattgtt
     2821 cacgtgtgta tgctgcagtg atcttcatgg tctgcctcat ggccgctcct gcctccccgt
     2881 ggccgcataa ccgccggctc accgtcatcg tcgcacctta ttcctcctcc cggtttcccg
     2941 acttccgcaa gaac
//
LOCUS       DATGFG010000736         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_306044, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000736 DATGFG010000000
VERSION     DATGFG010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306044"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VKQ52_13725"
     CDS             <1..351
                     /locus_tag="VKQ52_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88307.1"
                     /translation="SGVASGINNAMTRISSVFANAIFGALAVLLFTGVLQGRLEGMSL
                     SPEVKQAVLAQAANLGNAKPPSALAPQDKPVVERMYREGFIVVYGDILRLAAGLAFLG
                     ALMGLLFVKRARGR"
     gene            421..2034
                     /locus_tag="VKQ52_13730"
     CDS             421..2034
                     /locus_tag="VKQ52_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88308.1"
                     /translation="MRYSRCWFVLFLSLGGLFAMGQPLSNDSLLAIIRQNKQDTAEAK
                     ALNAIAANLSRSDIPRGIGYLRQLIDLCVRIKDTGYLSRGYNQLVTSHLNTGRSDSAQ
                     YYLSLLKKMSEWPGATEARADYHAAAGLFYKIQGNYKAAMPFLLASLEDAASIAKKDP
                     SPGTRTYLAGQYLNIGNTYALMADYRKALSYHLQSLQLFEDAGNKRGISYSYQGVGGD
                     FLSLRQFSQATDYTKKAIALKTEIKDQRGIGTALKQQGSIFRGQKQWDSTLVYYFQAL
                     KAFQAMNLKVEETDMDFDIGNVYGDKSDLPNARIFLNDSKAVAREIGDSSRFKTADAA
                     LVSLQGRVDRQQQDEVKLMSSLHASVESGDRQMELANYQYLSDHYAGIKQYDKALYYA
                     RKYYEMNDSLQGRDVQVQMERLEAQYNVGKKEQEIALLKKDQDLTRVELQRQKVFQYG
                     AVLVLALLVLIAFLIFNRWRIVHNARRMIELEKMRNTIARDLHDDIGSTLKHQCAEQG
                     SAGAAGGGIYAEQPAEDQGPEFGDHGEDG"
BASE COUNT          481 a          529 c          678 g          458 t
ORIGIN      
        1 tccggggtgg cgtcggggat caacaatgcg atgacgcgga tctcgagtgt ctttgccaat
       61 gccatcttcg gagcgctggc agtcttgttg ttcacggggg tgttgcaggg gcgattggaa
      121 ggaatgtccc tgtcgccgga agtgaaacag gccgtgctgg cgcaggcggc gaacctgggg
      181 aatgcaaagc cgccttcggc ccttgcgccg caggacaagc ccgtggtgga gcggatgtac
      241 cgggaggggt tcatcgtagt gtacggggat atcctgcggc tggcggcggg gttggccttt
      301 ctgggggcgt tgatggggtt gttgtttgta aagagggcgc ggggacgata gttggagaat
      361 agcagggttc ccatttattt tgtactttat cccgccgtca tgtccatcaa aaagcccatt
      421 atgcgctatt cccgatgttg gttcgtctta tttttgtcgc tgggcgggct gtttgccatg
      481 ggtcagcccc tatccaacga cagcctgctg gccatcatcc ggcagaacaa acaggatacg
      541 gccgaggcga aggcgttgaa cgccatagcc gccaatctta gccggagcga tatacccagg
      601 ggcatcggct atcttcgcca attgatcgac ctttgcgtcc ggataaaaga taccggctat
      661 ctcagccgcg gctacaatca actggtgacc tctcatctga ataccggcag gtccgacagc
      721 gcccaatatt atctttccct cctgaaaaag atgtcggagt ggccgggcgc taccgaggcc
      781 agggccgact atcatgcggc cgcggggctt ttctacaaga tacagggcaa ttataaggcg
      841 gcgatgccct ttctgcttgc ctccctcgag gatgccgcgt ccatcgctaa aaaggatccc
      901 tcgcccggca ccaggaccta tctggcggga cagtatctta atatcggcaa tacctatgcg
      961 cttatggcgg attaccgcaa ggcgctcagc tatcatttac aatccctgca gcttttcgaa
     1021 gatgcgggta acaaacgggg tatctcttat tcctaccagg gtgtgggcgg ggattttctt
     1081 tcgctgagac aattcagcca ggccaccgac tatacgaaaa aagccatcgc gctaaagacc
     1141 gaaataaagg accagcgggg aatcggcacc gcgttgaagc agcaggggtc gatcttccgg
     1201 ggccagaaac agtgggattc caccctcgtc tattatttcc aggcactgaa ggccttccag
     1261 gcgatgaacc ttaaagtgga ggagacggac atggatttcg atatcgggaa tgtctatggc
     1321 gacaagtccg atcttcccaa tgcgcggatc tttctcaacg acagcaaggc cgttgcccgg
     1381 gagatcgggg acagttcgcg tttcaagacc gccgatgccg ccctggtttc gctgcagggc
     1441 agggtcgacc ggcaacagca ggacgaggtg aaactgatga gcagtctgca tgcctcggtg
     1501 gagtccgggg atcgccagat ggagctcgcc aattatcaat acctgtcgga tcattacgcg
     1561 gggatcaaac aatacgataa ggcattgtat tatgcccgga agtattatga gatgaatgac
     1621 agcctgcagg gcagggacgt gcaggtgcag atggaacggc tggaggcgca gtacaatgtc
     1681 gggaagaaag aacaggagat cgcgctgctg aagaaggacc aggatctgac gcgggtggag
     1741 ttgcagcggc agaaggtgtt tcagtatggg gcggtgctgg tgctggccct gctggtgctg
     1801 atcgcctttc tgatctttaa ccgatggcgc atcgtgcata atgcccggcg gatgatcgag
     1861 ctcgagaaga tgcggaatac gatcgcgcgc gatctgcatg atgatatcgg gtctacgctg
     1921 aagcatcaat gtgctgagca aggtagcgct ggagccgcag gaggagggat ttatgcggag
     1981 cagcctgcag aagatcaagg accggagttc ggcgatcatg gagaagatgg atgatatcgt
     2041 atgggcgata aatccgcaga acgatacgat ggagcagttg ctggcgcgga tgaaggagtt
     2101 tgcggccgag ctgctggagc cgtcgaatat caattaccgc ttcgag
//
LOCUS       DATGFG010000737        10141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_234087, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000737 DATGFG010000000
VERSION     DATGFG010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234087"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VKQ52_13735"
     CDS             complement(<1..554)
                     /locus_tag="VKQ52_13735"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ88309.1"
                     /translation="MKISLVPALILMLVLCSYASDSRGQKLLEKKVTLQLVDEELRNV
                     LTALEKQTDVKFVYSPELIGASRKVSVDAKEKELYKVLTDLLGSLNIGYELVTNYVVL
                     SRKTARNLTFSSYGMIPDNSPYAFVKVGGRVVNGAGQPLEGVSVVVKGTNIGTTTNAN
                     GEFSLNVPDRQSVLVISYIGYQSQ"
     gene            complement(735..1856)
                     /locus_tag="VKQ52_13740"
     CDS             complement(735..1856)
                     /locus_tag="VKQ52_13740"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ88310.1"
                     /translation="MTSKRDKLYMANEEYNTLEDLVFSHSFRNWVLKGDTPEADFWSN
                     WVARNPEKTGMVNHAKAIIFALQLNLRPLSDDAVDTEVRKVLQKLKDGRLNLIREIPF
                     RPGILGRRPARAWTIAAAIAGIFILVWSVRIFFPRPQEKFYQSFLAATTTKPVRRQTG
                     DADSTKNLTLPDGSTVRLTRGSKLSFPDGLLTANHRREVFLEGEAFFNITHDASAPFY
                     VYTRSVITKVLGTSFTVKTTAEARTIVAVTSGKVSVYRKIDLSDGVILTPNQQITYTP
                     GADRLDRSITDQPRPLAGLADTTLAFNATPMATVFRRLQTLYGISILFDEETVTGCSL
                     SVTMGKESFYEKLNIICKAIDASWEAIDGNIVITAPGCK"
     gene            complement(1902..2495)
                     /locus_tag="VKQ52_13745"
     CDS             complement(1902..2495)
                     /locus_tag="VKQ52_13745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ88311.1"
                     /translation="MASVNLLDENLLLWNEFRNGNADAFGELMRTHYRDLFNYGTRFT
                     KDTELVKDCIQDLFLALWLHRLTISETTFVKYYLLKSLRHDLTRAITRSRHREEAHFQ
                     DLFGSAPPIENHLIREEHLADLARKMRKALAGLTKRQQEVIYLRFYMDADTDEIADIM
                     SLSRQSVYNLLHDALRRLRKVSPKKAFWFPHLLPLLF"
     gene            complement(2551..3222)
                     /locus_tag="VKQ52_13750"
     CDS             complement(2551..3222)
                     /locus_tag="VKQ52_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88312.1"
                     /translation="MKDSLKQYDSLLVIDDHNMIVNGVRLLIGARFREMWQANDGSGG
                     LALAIRHQPQLVIVDYVLPDGTGDTIVREIRYRCPATRILAYSFNGYSAAVLKMFGAG
                     VHGYVIKSEDDEEFVRAVSTLLEGKEFFCREARNHIIHRVFPEEERPRLIVANTGFSA
                     KEIEIIRLICQQKTAREISNQVFLSERTVEQYRSNISKRIGARNVAGIIRFALQNHII
                     DLADL"
     gene            complement(3219..5060)
                     /locus_tag="VKQ52_13755"
     CDS             complement(3219..5060)
                     /locus_tag="VKQ52_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88313.1"
                     /translation="MRRSFLLKPFLIFLGILAGHSLRAQTADSLKHRLSALLVQYKAR
                     LLKDTDYLRCVDSIAPLLESDDSLPEALSTYRQIAFGDPKQGRRRANYYTYLALNAYN
                     ANKWGTAIYYSEKNNEERVNAGLFEKGELAHSDLFALSLYYNNRDYPRVIMKLLTLNP
                     ALGRIPPAISAGKAGPEQVFLALSILETAVYTYARTGDTPARLTAFRLAETIQQEVRA
                     APGKYTRFIPQYNYLQHSTAYRNEQAQGHSEQARKLLLAAITDVEGPDFPKNLKADYS
                     VSLYTEAVDFYFDRNQVDSARHYLDILHGHGTNALFAVTDPGFLLISDSRLLAGEKKY
                     AEAYSSLRKAWLLRDSAFYTVSSDRDNNLYALAEAENTRAELFRSEESKRAAQQSNLL
                     LFFILSILVLGGISIFIVYRAQQQQRLLNLQLHLARNFHDTVGPMLLYANALVKKEAE
                     QRPSEGLDELKGQIGQIMEAVRSISHDLKTNRLGTLNTLGKELTTLLEKIRDATGIAF
                     TLTVDNGQLVLSHFQKTHLSKIIQELITNSVKHSACSRISLAIKGQPRNLQLSYSDDG
                     KGMDPAVPTTGIGLANIRERVDSLQGRLELVNDFPKGYSIALSIPLL"
     gene            5190..5528
                     /locus_tag="VKQ52_13760"
     CDS             5190..5528
                     /locus_tag="VKQ52_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88314.1"
                     /translation="MNHFIDSYYSSVFNSVARLTGSSDENELKALTEEVLGHLRQREE
                     ELAAESHKGIFIYKVVLTHVFSFLERKRDENRIDFLQKILPIHPSHYGQLHAPDDKKP
                     PEPDEPSGGG"
     gene            complement(5569..5832)
                     /locus_tag="VKQ52_13765"
     CDS             complement(5569..5832)
                     /locus_tag="VKQ52_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88315.1"
                     /translation="MGKITTIEQLREAIRDLEHQNYVNEQHMRGRVADLADRLRPANV
                     IKGLFSHFFTGGNEMKTSLLKMAAGIATSFVVKKFFKKGLNPK"
     gene            complement(5838..6182)
                     /locus_tag="VKQ52_13770"
     CDS             complement(5838..6182)
                     /locus_tag="VKQ52_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88316.1"
                     /translation="MQEHSTTDIDILLSDAGDFIETRTTLWKYKTIESLADVSGELVS
                     GLGMIVISSFVIITFSIGFALLIGEWLGKSYYGFFIMGGIYTIVALVIFAGRRKWLKD
                     PFSNMLIRKILK"
     gene            complement(6191..6427)
                     /locus_tag="VKQ52_13775"
     CDS             complement(6191..6427)
                     /locus_tag="VKQ52_13775"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HLZ88317.1"
                     /translation="MNNSSKILLGFIAGAAIGGALGILLAPDKGSETRRRIVEKGSDI
                     GDSIADFGDTVKEKFNDVVDGVKGSFSKAKNSMS"
     gene            6597..8090
                     /locus_tag="VKQ52_13780"
     CDS             6597..8090
                     /locus_tag="VKQ52_13780"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006968.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HLZ88318.1"
                     /translation="MQNGTLFQYFHWYYPSDGSLWKKVAAEAPRLAEMGFTAIWLPPA
                     CKGTNATYSSGYDIYDLYDLGEFDQKNSVRTKYGSKQEYLEAIKAIHAAGMQVYVDIV
                     LNHKAGGDETELIRVRKVDPEDRTKFISEPFDIEAFTKFNFPGRKGKYSPFIWDFRCF
                     TGVDFDNKTKENAIYKILNDYGDGWEDVVDDEKGNYDYLMYDDIEFRNPAVREELKKW
                     GEWYCKTTGFDGVRLDAVKHISPAFENEWLDHMRKVTGKEFFAVGEYWAPGNLPLLLK
                     YIDATEGRMSLFDASLHHNFEAAGKQGRDYDLTTIFNDTLVAVRPDLAVTVAGNHDTQ
                     PLQSLEAPVEPWFKPLAYAILLLREAGYPCVFYPDMYGAKYKDKGHDGKEYEIYMPGV
                     ENIEKLVQARKRFAYGAQRDYLDHANCIGWTREGDGDHPKSACAVLLSNGDEGFKVME
                     IGKAHAGRTFTDWLGKHPAKVMIDAEGKGEFHVSGNSVSVWIEEGAG"
     gene            complement(8113..8490)
                     /locus_tag="VKQ52_13785"
     CDS             complement(8113..8490)
                     /locus_tag="VKQ52_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ88319.1"
                     /translation="MTDTNKVLIVDDELDICYLLSGMLKQRNFQAGFVNTLSDAKTAL
                     QTETPSLIFLDNHLPDGFGLEFIPFIKKNYPGAKVIMITAHDGAQERKQAYDGGVDLF
                     IAKPLNRKLIFDAVDKLLVVHPR"
     gene            8848..>10141
                     /locus_tag="VKQ52_13790"
     CDS             8848..>10141
                     /locus_tag="VKQ52_13790"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLZ88320.1"
                     /translation="MKRILIIDDDMDMCNLLGRFLQKKGYETEASHSGNKGIAKFKES
                     KFDVVLCDFRLGDKEGREVLREIKNVDPYAIVIIITGYSDIKTAVDVIKAGAFDYITK
                     PLIPEEVISVIGRALQQQPSEGGDVQKPSAASTQTTAARKPLKAASEEEYLVGQAPAT
                     KELYRQIELVAPTNYSIILYGESGTGKEVIARTIHANSARKDKPFIAMDCGTLSKELA
                     GSELFGHVKGAFTGALGDKEGHFELANGGTLFLDEVANLSVEIQAALLRVIQERKFKR
                     VGGTKEMETDVRIIVASNENLQEAYRKGRFREDLFHRFNEFSVVLPAFRHRKEDIPLF
                     ADFFLARANKELNKEVEGFEPEVMQIFHNYPWPGNLREFRNVIRRAALLTQSGRVNAR
                     VLPAEIVDVNGYGSYFVPQPTEAPAAAQTHHPIAHKETD"
BASE COUNT         2215 a         2301 c         3143 g         2482 t
ORIGIN      
        1 tcctggctct ggtagccgat gtaggagatg acgagaacgg attgccggtc gggcacgttg
       61 agggagaact cgccgttggc gttggtggtg gtaccgatgt tggtgccttt gaccaccacg
      121 ctgacccctt cgaggggttg gcccgcgcca ttaacgaccc tgccgccgac ttttacgaag
      181 gcataggggc tgttgtcggg gatcatgcca tagctgctga atgtcaggtt tctggcggtc
      241 tttctgctga ggacgacata gttggtgacg agttcgtacc cgatgttgag gctgccgagc
      301 agatcggtga gaaccttgta gagctccttt tcttttgcgt cgactgagac cttacgggag
      361 gcgccgatga gctcggggct gtagacgaat tttacatcgg tttgtttttc cagggcggtt
      421 aagacgttgc gaagttcttc atcgacgagt tgcagggtaa cctttttttc cagcaatttc
      481 tggccgcggg agtcgctcgc gtaggaacaa aggacaagca tcaaaatcag ggctggaacg
      541 agtgagatct tcataatggc aatagcaatt tgacgactca attgtttttt catactttac
      601 aatcgttttg gttcttaata atggagggcc aaacactggc tgacgatggg ctcaatcatt
      661 gtcagccaat ttttttaaag tgggtatggt cggttctttt attttatcat gcagtcgggt
      721 tttggatgag gaatttattt acagcccggg gcagtgatga cgatattgcc gtcgatggct
      781 tcccaggagg cgtctatcgc cttgcagatg atattcagct tttcataaaa agactctttt
      841 cccatggtca cggaaaggct gcagcctgtg acggtttctt cgtcgaagag gatggagatg
      901 ccgtagagtg tctgcaagcg ccggaagacg gttgccatcg gtgttgcgtt gaaggccagg
      961 gtggtatcgg cgaggccggc caggggtctt ggctggtcgg tgatcgacct gtcgaggcgg
     1021 tcggcgccgg gcgtataggt gatctgctgg ttgggggtga ggatgacccc gtcggagagg
     1081 tctatttttc tatagacgga taccttcccg ctggtgacgg caacgatcgt tctcgcttcc
     1141 gcggtggttt tgacggtgaa gctggtgccc agcacctttg tgatgaccga tcgggtatag
     1201 acatagaacg gggcggaggc atcgtgggtg atattgaaga aggcttctcc ctcgaggaag
     1261 acctcgcgcc ggtggttggc tgtcaggaga ccgtcgggga aggacagttt gctgccgcgg
     1321 gtgagtcgga cggtgcttcc gtcggggagg gtcaggtttt ttgtgctgtc ggcgtcgcct
     1381 gtttgccggc gaacgggttt tgtggtggta gcggcgagga aggactggta gaatttttcc
     1441 tgtggccggg ggaagaagat gcggacagac cagaccagga tgaagatacc ggcgatggcg
     1501 gcggcgatgg tccaggcgcg ggcggggcgg cggccgagga tgccggggcg gaaggggatc
     1561 tcgcggatga ggttgaggcg gccgtccttc agtttctgga gcactttccg gacctcggtg
     1621 tcgacggcgt cgtcggacag gggccgcagg ttcaactgca gggcaaatat gatggctttg
     1681 gcgtgattga ccatcccggt cttttccgga ttgcgcgcca cccaattgga ccagaagtcg
     1741 gcctcggggg tatccccttt cagcacccag ttacggaaac tatgggagaa gaccaggtct
     1801 tcgagggtat tgtattcttc gtttgccatg tatagtttat cccttttact tgtcataaag
     1861 acaggtatcg ggaatatcta ctggtttttt tgaaagaatt tttagaagag cagcggcagg
     1921 aggtgtggga accagaaggc ttttttgggg cttaccttgc gcaggcgccg gagggcgtca
     1981 tggaggagat tgtatacgga ctggcggctg agggacatga tatcggcgat ctcgtcggtg
     2041 tcggcgtcca tgtagaagcg gaggtagatg acctcctgct ggcgttttgt gaggccggcg
     2101 agggccttgc gcattttgcg ggcgaggtcg gcgaggtgtt cttcacggat gagatggttc
     2161 tcgatcgggg gggcgctgcc gaacagatcc tggaagtggg cttcctcgcg gtgccgggaa
     2221 cgggtgatgg cccgggtaag atcatgacgg agagatttga gaagatagta tttgacaaaa
     2281 gtcgtctcgc tgatggtgag gcggtgcagc caaagtgcaa gaaagaggtc ctggatgcag
     2341 tcttttacga gttctgtgtc ttttgtgaag cgggtaccgt agttgaacag atcgcggtag
     2401 tgggtgcgca tcaattcgcc aaaggcatcg gcgttgccgt tgcggaactc attccagaga
     2461 aggaggtttt cgtccaatag attcacggag gccattgcag tcgttagttt ggcgggtggt
     2521 gccaataaag ataagaatta actgttgttt ttacaagtcc gcgaggtcga tgatgtgatt
     2581 ttgcagggcg aagcggatga tccccgccac attgcgggcc ccgatccgtt tggagatgtt
     2641 gctgcggtat tgttccacgg tcctttcgga gaggaagacc tggttgctga tctccctggc
     2701 cgtcttttgc tggcagatca gccggatgat ctcaatttcc tttgcggaga agccggtgtt
     2761 ggcgacgatg agcctgggtc gttcttcctc ggggaagacg cggtgaatga tgtggttacg
     2821 cgcttccctg cagaaaaatt cctttccttc cagcagggtg gacacggccc tgacgaattc
     2881 ctcgtcgtcc tcgcttttga tgacatagcc gtgtacgccg gccccgaaca ttttcaggac
     2941 ggcggcggaa tagccgttaa aggaataggc gaggatgcgg gtggcgggac agcggtagcg
     3001 gatctccctg acgatggtat ctcctgttcc atcgggcagc acatagtcca cgatcaccag
     3061 ttgcggctgg tgccggatcg ccagggccag gccgccggag ccgtcgttgg cctgccacat
     3121 ttcgcggaac cgggcgccga tgagcaggcg gacgccgttg acgatcatat tatggtcgtc
     3181 gatcaccagg agggagtcgt attgtttcag ggaatccttc ataacagggg gattgagaga
     3241 gcaatggaat agccttttgg gaagtcgttg accagttcga ggcggccctg cagggagtcc
     3301 actctttccc tgatattcgc caggccgatg ccggtggtgg ggacggcggg gtccatgccc
     3361 ttgccgtcgt ccgaatagct gagctgcagg tttcgtggct ggccttttat cgccaggctg
     3421 atccggctac aagccgagtg tttgacggaa ttggtgatca gttcctggat tattttcgac
     3481 aggtgggtct tttgaaaatg gctgaggacc agttggccgt tatctacggt cagggtgaag
     3541 gcgatgccgg tggcatcccg gatcttttcc aggagggttg tcagctcctt cccaagggta
     3601 ttgagggtgc cgaggcggtt tgttttcagg tcgtgggaga tgctgcgcac ggcttccatg
     3661 atctgaccga tctggccctt tagttcatcg agtccttccg agggtcgttg ttcggcttct
     3721 ttttttacca gcgcgttggc atacaggagc atggggccga cggtatcatg gaagttgcgg
     3781 gccaggtgga gctgcaggtt gagcagccgt tgctgttgtt gggcgcgata gacgataaaa
     3841 atgctgatgc cgcccaggac caggatcgag aggataaaga acaggagcag gttggattgt
     3901 tgtgcggcgc gtttgctttc ttccgaccgg aagagttcgg cccgggtgtt ctcggcttcc
     3961 gccagggcgt agaggttatt atcccggtcg gagctcacgg tatagaaagc cgagtcccgg
     4021 agcagccagg cctttcgcag ggaagaatag gcctcggcgt attttttctc gccggccagc
     4081 aggcggctgt cgctgatcag caggaagccc gggtcggtga cggcaaagag ggcattcgtg
     4141 ccgtggccat gcaggatatc gaggtagtgc cgggcgctgt ccacctggtt ccggtcgaag
     4201 taaaagtcca ccgcctcggt gtacagggag acgctgtaat cggccttgag gttctttggg
     4261 aaatcggggc cttccacgtc ggtgatagcg gcgagcagca gtttccgggc ttgttcggaa
     4321 tggccctgtg cctgttcatt tcgataggcc gtactatgtt gcaggtaatt gtactgcgga
     4381 atgaaccggg tatacttccc cggagccgcc ctcacctcct gctggatggt ttcggcgagc
     4441 cggaaggcgg tgagccgggc cggggtatcc cccgtcctgg cgtaggtgta gacggcggtc
     4501 tcaaggatgc tcagggccag gaagacctgt tccggtcccg ctttcccggc gctgatggcc
     4561 ggcggtatcc ggccaagggc ggggttgagg gtcaggagct tcatgatcac gcggggatag
     4621 tcccggttat tataatagag cgagagggca aacaggtcgc tatgggccag ttcccctttt
     4681 tcaaaaagcc cggcgtttac acgctcctcg ttgttctttt cggagtaata gatggccgtt
     4741 ccccatttat tggcattata ggcattgagc gccagatagg tatagtagtt ggcccggcga
     4801 cgtccctgtt ttgggtcgcc aaaggcgatc tggcggtaag tggacagcgc ttccgggaga
     4861 ctatcatcag attccagcag gggagcgatg gaatccacgc atcggagata gtcggtgtcc
     4921 ttcaggagac gggctttgta ctgtaccagg agcgccgaca gccgatgttt cagggaatcg
     4981 gccgtctggg cgcgtaggga atggccggca agaatgccca ggaatataag aaatggtttt
     5041 agcaggaacg atcgccgcat aatcaaatgt aaataaaaaa gtatataata gaacttgttc
     5101 aatccgaatt gcggaaaatt caggactttt ttgtctctag ccgaagctac aataacttcc
     5161 ccgcagctta ctaattcaac acctacttat tgaaccattt tatcgattct tattactcat
     5221 cggtctttaa ttctgtcgcc aggctcacag ggagttccga cgaaaatgaa ctaaaggccc
     5281 tgacggagga agttcttggc cacctgaggc agcgggagga ggagctggcc gccgagagcc
     5341 ataaagggat ttttatctac aaggttgtcc tcacgcatgt tttttccttt ctggagcgga
     5401 agcgggacga gaaccgtatc gactttcttc aaaagatact gccgatccac ccgtcgcatt
     5461 atgggcagct gcatgcgccg gatgataaaa aaccccctga accggacgag ccatcagggg
     5521 gaggttgata tatagggtga ctgttgccgt ctgttcccga tcttgcgctt atttggggtt
     5581 cagccctttt ttaaagaatt tcttcacgac gaaactggtg gcgatgccgg ccgccatttt
     5641 cagcaggctt gtcttcattt cgttaccacc ggtgaaaaag tggctgaaca gccctttgat
     5701 gacgttggcg ggtcttaacc ggtcggccag gtcggctacc cggccgcgca tgtgctgttc
     5761 gttgacataa ttctgatgtt ccaggtcgcg tatcgcttcc cgcaattgtt cgatggtagt
     5821 gatcttaccc atggcgctta ttttaggatc ttccggatca gcatattact aaaggggtct
     5881 ttcagccatt tccgcctgcc ggcgaaaatg accagggcga cgatggtata gatgccgccc
     5941 atgatgaaga acccataata gcttttaccc aaccactccc cgatcaaaag agcgaatccg
     6001 atgctaaagg tgatgatcac gaaggaagag ataacgatca tgcccagccc agagaccaat
     6061 tcgccggaga catcggcgag actttcgatc gtcttgtatt tccacaaggt ggtgcgggtc
     6121 tcgataaagt ctccggcgtc ggacagcaga atgtcgatat ccgttgttga atgttcttgc
     6181 ataagtccgg ttaagacatt gagttttttg ccttgctgaa gcttcctttc acgccatcga
     6241 ccacatcgtt gaatttttct tttacggtgt cgccaaagtc tgcgatggag tcgccgatgt
     6301 cggaaccttt ttcgacgatc ctgcgacggg tttccgagcc tttatccggg gccagtaaga
     6361 tgcctaatgc gccaccgatc gctgcgccgg cgatgaagcc gagtaaaatt ttcgatgagt
     6421 tgttcataaa cgttgatttt agctattata gtcaaaattc cttgccaaat ttttgaagag
     6481 ggagaattcg gggtttggcc cgggtgttat gagaaaaata ttctacaatc tgagcaagag
     6541 ggggattgtt ccgggagcgc agggagtgtg gtattattct ggttatagac ttgtttatgc
     6601 aaaatggaac cctattccag tatttccact ggtattaccc ctcagacggt agtttatgga
     6661 agaaagtagc ggcagaggct ccgcggctgg cggagatggg atttacggcc atctggctgc
     6721 cgccggcctg caaaggaacg aatgctacgt attccagcgg ttatgatatc tacgacctgt
     6781 atgatctcgg ggaattcgat cagaagaatt cggtacgaac gaaatacggg agcaagcagg
     6841 agtacctgga ggccatcaag gcgatccatg cggccgggat gcaggtgtat gtagatatcg
     6901 tgctcaatca caaggccggt ggcgacgaga ccgagcttat ccgcgtccga aaggtcgatc
     6961 ccgaggacag gacaaaattc atcagcgaac cattcgatat agaggcgttc acgaaattca
     7021 attttcccgg gcgtaagggg aaatacagtc cttttatctg ggatttccgg tgtttcacgg
     7081 gtgtcgattt tgataataag acaaaggaaa acgccattta taagatcctc aatgattatg
     7141 gcgatggctg ggaggatgtc gtcgacgatg agaaaggcaa ctatgactat ctcatgtatg
     7201 acgacatcga gttcaggaat ccggcggtgc gggaagagtt gaagaaatgg ggggaatggt
     7261 attgtaagac gacggggttt gacggggtgc ggctggatgc cgtaaaacat atctcgccgg
     7321 cattcgagaa cgagtggctg gatcacatgc gaaaggtgac ggggaaggag ttctttgccg
     7381 tcggggaata ctgggcgccg ggcaatctgc cgttgttatt gaagtatatc gatgccacgg
     7441 aagggcggat gtcccttttc gatgcttccc tgcatcacaa tttcgaggcg gcgggtaagc
     7501 aggggcggga ttatgacctg accaccattt tcaacgacac cctggtggcg gtgcggcccg
     7561 atctggcggt gacggtggcc ggcaaccatg acacgcagcc cctgcaatcg ctggaggcgc
     7621 cggtggagcc ctggttcaag cccctggcct atgctatttt gttgttgcgg gaggcgggct
     7681 acccctgtgt tttttatccg gacatgtatg gggcgaaata caaggacaag ggccatgatg
     7741 ggaaggagta cgagatctat atgccggggg tagagaatat cgagaaactg gtacaggcga
     7801 ggaagcggtt tgcctacggg gcgcagcggg attacctgga tcatgcgaac tgcatcggct
     7861 ggacaagaga aggagacggc gatcatccga agtcggcctg cgccgtgtta ttgagcaacg
     7921 gtgatgaagg ttttaaagta atggagatag gaaaggcgca cgcgggcagg acctttaccg
     7981 attggctggg caaacatccg gcaaaggtga tgatcgatgc ggaagggaag ggagagttcc
     8041 atgtcagcgg gaattcggtg tccgtctgga tagaagaggg ggctggatga tccggctggg
     8101 ttgcatcagg gatcagcggg ggtgcactac caggagtttg tctactgcgt cgaagatgag
     8161 cttgcggttc agcggtttag cgatgaacag atcgaccccg ccgtcgtagg cttgttttct
     8221 ttcctgtgcg ccgtcgtggg cagtgatcat gatcactttt gcgccggggt aattcttctt
     8281 gataaaggga ataaactcga gtccgaagcc atccggaagg tgattatcca ggaaaataag
     8341 agaaggcgtt tccgtttgaa gtgccgtttt cgcatcgctg agtgtattta cgaagccggc
     8401 ctgaaagttc cgttgtttca acatcccact gagtaaatag caaatatcca attcatcgtc
     8461 tacgatcagg actttgtttg tgtctgtcat aataattgtt tccacttaac cttcaaaatt
     8521 tagaatccaa acaattttat ataactatct atttacaatt ttattgccat ttaatttttc
     8581 agtcgggcag cgtagtgaaa tagaccggtt ccgcttttaa tcatcccttg taagagtatt
     8641 ttcgtaaatt tagtggtgga ctccgcccca aactggggaa tttaataccc ataaaccgct
     8701 cagaagcctg tttaccaagc gactggaaat tggcaagggt tttagaatat aaagggtaga
     8761 caatcgataa tcggttgatt aatccctttt tatatttttt cttttcgcgg taattttaat
     8821 gcagattttg aaaatcaaat tatgactatg aagcgaattc tcatcattga cgacgatatg
     8881 gatatgtgta atcttctcgg ccgtttcctg cagaagaagg ggtatgaaac ggaggccagc
     8941 catagcggca acaaggggat cgcaaagttc aaggaatcca aattcgacgt tgtgctctgt
     9001 gatttccggc ttggtgataa ggagggaagg gaggtgttga gggagatcaa gaatgtcgac
     9061 ccttatgcga tagttattat cataacgggg tattccgata tcaagacggc ggtggatgtg
     9121 atcaaggcag gcgccttcga ctatattacg aagccgttga tccctgaaga ggtgataagt
     9181 gttatcggca gggcgttgca acaacaaccc tcggaaggtg gtgatgttca gaagccctcg
     9241 gcggcgtcga cgcaaacgac ggcggcacgg aagcccctca aggctgccag tgaagaagaa
     9301 tacctggttg gccaggcccc cgcgacaaag gaactgtacc ggcagataga gttggtggcc
     9361 ccaacgaatt atagtatcat tctctatggc gagagcggaa cgggtaagga agtcatcgcg
     9421 cggacgatac acgccaacag tgcgcgcaag gacaagccct ttatcgccat ggactgcgga
     9481 acgctgtcga aggagcttgc gggcagtgaa ttattcgggc atgtcaaggg cgcttttacc
     9541 ggcgcgcttg gcgacaagga ggggcatttc gagttggcca atggcgggac cctgttcctg
     9601 gatgaggtgg ccaatctctc cgttgagata caggcggccc tgttgcgggt gatccaggag
     9661 cggaagttca agcgagtagg cggcacgaag gaaatggaga ccgatgtccg gatcatcgtg
     9721 gcctccaatg agaacctgca ggaggcttac cggaagggga ggttcaggga agacctgttc
     9781 catcgattca atgaattctc cgtcgtgtta ccggccttcc gtcaccggaa ggaggacatc
     9841 ccgttatttg ccgacttctt cctggccagg gccaataaag aattgaataa ggaggtggaa
     9901 ggtttcgagc cggaggtaat gcagatattc cacaattatc cctggccggg taatctccgg
     9961 gagtttagga atgtcatccg ccgggccgcg ttgctgacgc agtcgggccg ggtcaatgcc
    10021 cgggtattgc cggcggagat cgtggatgtc aatggttatg gatcctattt tgtcccccag
    10081 ccgacggagg ctcctgcggc ggcgcagacg catcatccga tagcgcataa ggagaccgat
    10141 c
//
LOCUS       DATGFG010000738         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_354749, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000738 DATGFG010000000
VERSION     DATGFG010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354749"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VKQ52_13795"
     CDS             <1..1635
                     /locus_tag="VKQ52_13795"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLZ88321.1"
                     /translation="KELTEKGLTVLMLERGRPFEHIKDYKSADKNPWDLPHRGNATTG
                     MQKERPVLSRYWAAYGTAAHEFNIDYWANEKDCPYVEVKPFTWWRAYQMGGRSILWGR
                     QSYRWSDFDFEANLKDGVAVDWPIRYKDLEQWYTYVEKFAGISGSMEGLPQLPDGHYL
                     PPMDLNCVEKDVAQRLKTAFRGQRHMFIGRTANLTAPIPGRTQCQFRNRCWEGCPFGG
                     YFSTQSSTLPAALATGNLTVRPFSIVTRILYDKDRKRATGVEVLDAETNKTYTYNSKI
                     VFLNASALNSAWVLMNSATDVWPDGLGSSSGELGHNVMDHHYMLGAKGSVEGFEDKYY
                     YGRRANGFYIPRFANLFGDKRDFLRGYGYQGSASREGWSRDVAEMNIGSEYKTALTEP
                     GPWEIGATGFGEILPYHENRIYLDKQVKDKWGLPVLAMDAELRENELKMRKDIVKELV
                     AMFEAAGVKNISTWDSDKYAIGQGIHEMGTARMGRDPKTSVLNAHNQVWDAPNVFVTD
                     GACMTSAACQNPSLSYMALTARAADFAVGELKKGNI"
     gene            1667..>2381
                     /locus_tag="VKQ52_13800"
     CDS             1667..>2381
                     /locus_tag="VKQ52_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ88322.1"
                     /translation="MLIVGAGCNRQEKTLFSLLPADRTGIHFQNRVVDTDTLNILDYL
                     YYYNGGGVAIADFNRDGLSDVFFVSNQGANALYQNKGGMHFEDITARAGVQGKGNWKT
                     GVTVADVNGDGWPDIYVSEVGGYKGFQGRNELFINNGPGKDGVVSFTERAKEYGLDVL
                     GFNTQAVFFDYDHDGDLDVFIVNHSVHSTESYADTSQRRVHSDMSGDKLFRNDSVDGG
                     RHFVEVTQAAGIYSSVVGYG"
BASE COUNT          519 a          494 c          846 g          522 t
ORIGIN      
        1 aaggagctga ccgagaaagg gcttacagta ttgatgctcg agcggggccg gccttttgag
       61 catatcaagg actataaaag cgccgacaag aatccctggg atcttccgca ccggggtaat
      121 gccaccacgg ggatgcagaa ggagcggccg gtgctttccc gttattgggc ggcttatggg
      181 acggcggcgc atgagttcaa tatcgactat tgggccaacg agaaagattg tccctatgtc
      241 gaggtgaaac cgtttacctg gtggcgggcg tatcagatgg gagggcggtc gatcctctgg
      301 gggcggcaga gttaccggtg gagtgatttt gatttcgagg ccaatctgaa ggatggggtt
      361 gcggtggatt ggccgatccg ctataaggac ctggagcaat ggtacaccta tgtcgagaag
      421 tttgcgggta tcagcgggtc gatggagggg ttgccgcaat tgccggatgg gcattatctg
      481 ccgccgatgg acctgaactg tgtggaaaag gatgtggcgc aacggttaaa gaccgccttc
      541 agggggcagc gccatatgtt tatcggcagg acggcgaacc tgacggcgcc gataccgggg
      601 cggacgcagt gccagtttcg caatcgctgt tgggaaggtt gtccttttgg cgggtatttc
      661 agcacgcagt cttcgaccct tccggcggcg ctggcgacag ggaatctgac ggtgcggccc
      721 ttctccatcg tgacgcggat cctttatgat aaggaccgga agcgggctac gggggtggag
      781 gtgctggatg ccgagacgaa taaaacctat acgtataatt cgaagatcgt atttttgaat
      841 gcctcggcgc tgaacagcgc gtgggtgctg atgaattcgg cgacggatgt ctggccggac
      901 gggcttggca gtagcagcgg ggagcttggg cataatgtga tggaccatca ttatatgctt
      961 ggggcgaagg ggagtgttga ggggtttgag gacaagtact attatggccg gcgggcgaat
     1021 gggttttata tcccgcgatt tgccaatctc tttggggaca agcgggattt tctgcggggg
     1081 tatggctacc agggtagtgc cagccgggag gggtggagcc gggatgtggc ggagatgaat
     1141 atcgggtcgg aatataagac cgcgttgacg gagcccgggc cctgggagat cggcgccacg
     1201 gggtttgggg agatactgcc gtatcatgag aacaggatct atcttgacaa gcaggtgaag
     1261 gacaagtggg gattaccggt gctggcgatg gatgcggagc tgcgggaaaa cgaactgaag
     1321 atgcggaagg atatcgtaaa ggagctggtg gccatgttcg aggcagcggg agtaaaaaat
     1381 atcagcacct gggatagcga taagtatgcg atcgggcagg ggatccacga gatgggaacg
     1441 gccaggatgg ggagggaccc gaagacatcg gtcctcaatg cgcataacca ggtgtgggat
     1501 gcgccgaatg tttttgtaac ggacggggcc tgtatgacca gtgccgcctg tcagaaccca
     1561 tcgctgtcct atatggcgtt gacggcgcgg gccgcggatt ttgcggtggg ggaattaaag
     1621 aaggggaata tctgatttgc gtaggaaact tttatatttc atatcggtgt tgattgtggg
     1681 tgcgggttgt aaccggcagg aaaagacgct tttctccctg ctgccggccg accggacggg
     1741 gatccatttt cagaacaggg ttgtggatac ggacaccctg aatatcctcg attatctcta
     1801 ctattataac ggcggcggag tggcgattgc cgatttcaac cgggatgggt tgtccgatgt
     1861 tttttttgtc agcaaccagg gggcgaatgc cttgtatcag aataaggggg ggatgcattt
     1921 cgaggatatc accgcgcggg cgggggtgca ggggaagggc aactggaaga cgggggtgac
     1981 ggtggccgat gtcaatggcg atggctggcc ggatatctat gtttcggagg ttgggggata
     2041 taaggggttc caggggagga atgaactgtt tatcaataac gggcccggga aggatggcgt
     2101 cgtcagcttt accgaacggg cgaaggagta tgggctcgat gtgctgggat tcaacacgca
     2161 ggccgtgttc ttcgactatg accatgacgg agacctggat gtttttatcg tcaaccattc
     2221 ggtgcattcc acggaaagct atgccgacac cagtcagcgg cgggtgcaca gcgatatgag
     2281 cggggacaag ttgttccgta atgacagtgt cgacgggggc aggcattttg tggaagtgac
     2341 ccaggccgcg ggcatctata gcagtgtggt cgggtatgga t
//
LOCUS       DATGFG010000739         6619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1056763, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000739 DATGFG010000000
VERSION     DATGFG010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056763"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VKQ52_13805"
     CDS             <1..933
                     /locus_tag="VKQ52_13805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88323.1"
                     /translation="KSYFTNPTRAEDGIRLVLKETKVTDYELTARHKNGEETVVSYNA
                     TTFYDRDGKLQGVFAAARDVTDRKRFEETLQEANRMKSEFLANMSHELRTPLNGIIGF
                     SEFLVDKKAGPVSHKQEEYLNDILNSGNHLLNLINDVLDLAKVEAGKMELITETFSVR
                     NAIEEVSSVVNGIARKKNIKIDIRIDNDMQDVTIDKHKFKQILYNLLSNAIKFTPEEG
                     SVTVTAGSCGNNCLSLQVKDNGIGMTEDGLHKLFTPFVQLDSSLTRKHEGSGLGLALT
                     KKFVELHNGRITAESEWNHGSTFTVVIPKYVLAN"
     gene            954..1361
                     /locus_tag="VKQ52_13810"
     CDS             954..1361
                     /locus_tag="VKQ52_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ88324.1"
                     /translation="MNPCILVVDDNPTNLKLAGDLLELEAFEVRRAADAESALEMIRS
                     KHPDLILMDVALPGMDGLTLTRVLKQSPGTEHIVIVALTAFAMKGDHEKILAAGCDTY
                     IRKPINTRSFPGQVKKILEDKTGTAAIAEELIT"
     gene            1373..1762
                     /locus_tag="VKQ52_13815"
     CDS             1373..1762
                     /locus_tag="VKQ52_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ88325.1"
                     /translation="MKIKALIVGDDQTGNNLLKAILEYEGIQSSEAGNGLEALEILAK
                     MEVGLIISDAHMPAMDGFELISAVRKKEGLAHIKFILYTATCTSPATEASAYKLGVDR
                     YVRKCGSTQNITSAITQLLHEHEDALV"
     gene            1762..2457
                     /locus_tag="VKQ52_13820"
     CDS             1762..2457
                     /locus_tag="VKQ52_13820"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ88326.1"
                     /translation="MSVPQSHTLDLTLLARLYQAREEERCTLAGELHDRLSQQLASLK
                     MDIFRLDRKLQPKDDYLTKKLTGMMELVDAAVESIRNISAELHPGVLDDAGLAAAMEW
                     YTREFIRQFAVQAAFTSDPLPFGIIPEISINLFRIYQGLLARSLTSPGLQQITASLVC
                     TDISLMLTLREDRWATIIEHPQTRPMLPDLAGRILLLSGECSFDDKPGNASSICICIP
                     IEKLLTPYLKPIS"
     gene            2454..3080
                     /locus_tag="VKQ52_13825"
     CDS             2454..3080
                     /locus_tag="VKQ52_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88327.1"
                     /translation="MKILIADDHSLVRRGLKETLLDEFPSSVVEEAEDAEGIFIKMST
                     QTWDLVICDLSMPGRSGLDVVHDLRVKFPKLPVLVVSTHAEEHYAVRSLKAGASGFLS
                     KTAPPAELITAINRLLQGRKYITPAIAERLATLMLDNSGQPPHELLSDREFDVSKQIA
                     SGKQVSEIAAVLSLSINTISTYRNRILDKMNLRTNAELTKYMIENHLV"
     gene            3302..3673
                     /locus_tag="VKQ52_13830"
     CDS             3302..3673
                     /locus_tag="VKQ52_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88328.1"
                     /translation="MENHKLIVLIVDDSLFTNIRMEEMLKELENVQQVLHAGSFEEGI
                     SLVNRESPDIVLLDINLPDMSGIELLKLIKEKYPAMRVVMVSNQAYEYHRELCKSLGA
                     LQFFDKTREFHRIPELISGIN"
     gene            3725..4423
                     /locus_tag="VKQ52_13835"
     CDS             3725..4423
                     /locus_tag="VKQ52_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88329.1"
                     /translation="MKTGTRLNQALEQQQILIPVTAQTSREDHVDEIITYMQDMERRR
                     LAIALRDNINQVLAAARIFAGLLEPMDEKQQEIKNRMAESLALAIGEIGKLTDERSGS
                     LFLETGLIDSINTLIDEVGLMHFDKIRFTCHNKNIERLQTSQKIVMFRIIRESLEQIV
                     ESNLLKDVELYLQFTGNRVLIVFDGTLRRTTAKNLARSFGLSYIGDLLRIYNGAVKLT
                     TVAQKSLLEISILL"
     gene            4550..5968
                     /locus_tag="VKQ52_13840"
     CDS             4550..5968
                     /locus_tag="VKQ52_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88330.1"
                     /translation="MTPEPPSLTRSGIVITKDGTIVPANGHLSQEPSPASAGIPETNL
                     FNLLPGRHRSFLKTMLNRADRCKSSQVSMRFDNSRPGDSEWEVIKLFAAGPAAPVYLC
                     LVPLHDAAALGPSDASQSIKCKALDERAASGIIVEDENGGILWANQKSARLLGVPPNE
                     LFTPHFLEKLWKTYHHVHGSVPFEANSPSGSRRPGLLPSSATLVIKQVDRKYKTLSFE
                     LEEISRRNFSPSLVLVRIKEIMAGEAESSPVISNDSDWVWMRREYIEKTIAKAKEAER
                     IRIGHELHDNINQMLAVIGVYLSLLHPADPDEKDLLGKSGHILKMAIEEIRCLSHGLV
                     KDSTLREGFIAAVTALVDDFRQTRMFNISFTFNNQEVESMDQSKKVALYRILQEHLNN
                     IVKYSQAKNVQICLDTDQKGVRLTIEDDGVGFNKLTVQKGIGLTGIYEKAELCNGSVE
                     LQTSPGHGCILKVTAMQGVSEN"
BASE COUNT         1832 a         1645 c         1604 g         1538 t
ORIGIN      
        1 aagtcctatt tcacgaaccc aactcgtgca gaagacggca tccggctggt gctcaaggaa
       61 acgaaagtca ccgattacga gctcaccgcc cgacacaaaa acggcgagga aaccgtcgta
      121 tcctataatg ctaccacctt ctacgatcgg gatggcaagc tccagggggt atttgcagcc
      181 gcccgcgatg tcaccgaccg caaacgattc gaggaaaccc tgcaggaagc caatcggatg
      241 aagagtgaat tcctggccaa tatgtcgcac gaactgcgca cgccattaaa tgggatcatc
      301 ggcttctcgg aattcctggt tgataaaaag gcggggcctg tcagccacaa gcaggaggag
      361 tacctcaacg acattctcaa cagtggtaac catttgctca atctgatcaa cgacgtactc
      421 gatcttgcca aagtggaagc cgggaaaatg gaactgatca cggaaacatt ttctgtaaga
      481 aacgccatcg aggaagtcag ctccgtggtc aatggcatcg cccgcaagaa gaacataaaa
      541 atagatatcc gcatcgacaa cgatatgcag gatgtcacca tcgacaaaca caaattcaaa
      601 cagatcctgt acaacctgct ctccaatgcg atcaagttca cgcccgaaga aggcagcgtc
      661 acggttactg ccggatcttg cgggaacaac tgtctctccc tgcaggtaaa agacaacggg
      721 atagggatga cagaggacgg tttacataaa ttgttcactc catttgtaca gttggattcc
      781 agcctgaccc ggaagcacga gggctcggga ttgggattgg ccctgacaaa aaaattcgtg
      841 gaattgcata atggaaggat cacggccgaa agcgaatgga accatggcag cacctttacc
      901 gtcgttattc caaaatatgt cttagccaat taaaatcaac caacaactta tctatgaacc
      961 cttgtattct tgtagtcgat gataatccta ccaatctcaa actggcaggc gatctgctgg
     1021 agctcgaagc cttcgaggtg cgacgggcag ccgatgcgga atcggcgctg gaaatgatca
     1081 ggagcaagca tcccgacctg atcctcatgg acgtagccct gcccggcatg gacgggttga
     1141 cacttacccg tgttctaaag caaagcccgg gtaccgaaca cattgtcatc gttgcgctca
     1201 ctgccttcgc tatgaaggga gaccacgaga agatcctggc cgccggctgt gacacctata
     1261 taagaaagcc gatcaacacc aggagctttc ccggccaggt gaagaagata ctggaagata
     1321 aaaccgggac agccgcgata gcagaagaac taataacata aaataataaa tcatgaaaat
     1381 taaagccctt atcgttggcg atgaccaaac aggtaacaac ctgttgaaag ccatactcga
     1441 atatgaaggt atccagtcct cggaggcagg aaatgggttg gaagcgctgg aaatactcgc
     1501 aaaaatggag gtcggtctca tcatttcaga tgcccatatg cccgctatgg atggattcga
     1561 gctgatctcc gccgtccgaa agaaagaagg cctggcgcac atcaaattta tcctctatac
     1621 ggcaacctgc acttctcccg ccactgaagc ttcggcttat aaattgggag tcgacagata
     1681 cgtacgcaaa tgcggtagta cacagaatat aacttccgcg attacccagt tattgcacga
     1741 acatgaagac gccctggtat aatgtcagtt ccgcaatcgc atacgcttga tctcactctg
     1801 ctggctcgcc tttaccaggc gcgggaggaa gaacgttgca ccctggccgg tgaattgcac
     1861 gatcgtctca gccagcagct ggcaagcctg aaaatggata tcttccggct tgacaggaag
     1921 ctgcaaccaa aagatgacta tttaacaaag aagctcacgg ggatgatgga gctcgtagat
     1981 gctgcggtcg aatcgataag gaacatcagc gccgagctgc acccgggagt tcttgacgat
     2041 gccgggcttg cggcagcgat ggaatggtat acgagggaat ttatccggca atttgctgtt
     2101 caggcagcat ttacatccga tccgcttcct ttcgggatca ttcccgagat atcgataaac
     2161 ttatttagaa tatatcaggg ccttctggcc agatcgctga cctctccggg tttacagcag
     2221 ataactgctt ctttggtatg caccgatata tccctgatgc tgacattgag agaagatcgc
     2281 tgggcgacga tcatagagca tccgcaaacc aggccgatgc tgccggatct tgccgggaga
     2341 atactactat tgtctggtga atgttccttt gatgacaagc cgggtaacgc atcgtcaatc
     2401 tgcatctgca ttccgattga aaagctatta accccttact taaaacccat atcatgaaaa
     2461 tcttaattgc cgatgaccat tcgctcgtca gaagaggtct caaggagaca ttgttggatg
     2521 aattcccgtc ctcggtcgtt gaggaagcgg aggacgcgga aggcatcttt ataaagatgt
     2581 ccacacaaac ctgggacctg gtcatttgcg atctctcgat gcccgggcgc agcgggctgg
     2641 acgttgttca tgatcttcgc gtgaaattcc ctaagcttcc tgtgctcgtc gtcagcacgc
     2701 atgctgagga gcactatgcc gtccggtcgt tgaaggccgg tgcatcgggg ttccttagca
     2761 agacggctcc accggcggaa ctgatcacgg ccattaacag gctcctgcag ggccgtaaat
     2821 atattactcc cgccatcgcc gagcggctgg caacgctcat gttggataat tccgggcagc
     2881 caccccatga gttgctgtcc gaccgggaat tcgacgtctc aaagcagatt gcatccggaa
     2941 aacaggtctc ggagatcgca gcggtacttt ccctcagtat caatacgatc agtacctacc
     3001 ggaaccggat cctcgataag atgaaccttc gaacgaatgc cgaactgaca aagtatatga
     3061 tcgagaacca cctcgtttga tcattctttc ccatctcagg ccacgcccca tgatcccatc
     3121 ggttcatggg gtttttaatt acccatctcc ctgctttacc aaccggtaca aaattccact
     3181 tattgccttg tagcacttgg ccctaccgac ggacaatgtc ggatggtgac ccgattatta
     3241 caggatgcac tatttttttc cggctcgttg cgccgtttct ttgtattgtc aaaacagccc
     3301 catggaaaat cataaattaa tagtgcttat cgtcgacgat tcactcttca ccaatattcg
     3361 aatggaagag atgcttaaag agctggaaaa cgtccaacag gtgttgcatg cagggagttt
     3421 cgaagaaggg atcagcctcg taaacaggga aagcccggat atcgttctgc tcgacatcaa
     3481 cctgccggat atgagtggca tcgagctgct gaagctgatc aaggagaagt atccggcaat
     3541 gagggtggtg atggtcagca accaggccta tgagtatcat cgcgaattat gcaagtcgct
     3601 gggcgccctt caattcttcg ataagacccg ggaatttcat cgcatccccg aattgatctc
     3661 cgggataaat tgaatgatat actcttttgc accttttgat catcttttaa ataaaaatgt
     3721 ctttatgaaa acaggcaccc gtttgaatca agcccttgaa caacaacaaa ttttaatacc
     3781 tgtcaccgcc cagacttcca gggaagacca tgtagatgag atcatcactt atatgcagga
     3841 catggaacgc aggcgcctgg ctatcgccct gagagacaat atcaaccagg tattggctgc
     3901 tgcccggata tttgccgggt tactggagcc tatggatgag aaacagcagg agataaaaaa
     3961 ccgtatggcg gaatccctgg ctttggctat cggggagatt gggaagctca ccgatgaacg
     4021 cagcgggtct ctcttcctgg aaaccggctt gatcgacagc atcaataccc ttatcgatga
     4081 agttggcctg atgcatttcg acaagatccg gttcacttgt cataacaaaa acattgagcg
     4141 gctgcaaaca tcccaaaaga tagtcatgtt cagaataatc cgggaatcac tagaacagat
     4201 cgtagaaagt aacctgctga aggatgtcga attatacttg cagttcaccg gaaatcgtgt
     4261 tctcatcgta ttcgacggta ccttacggcg aactacagca aaaaaccttg caagatcatt
     4321 cggtttgagc tacatcggtg atctgctgcg tatctacaat ggagccgtca aattgactac
     4381 ggttgcacaa aaaagtctac tggaaatcag catactatta taacagggtt tcgggtcaag
     4441 atgtgttgtt gtacaagtct catcccctgt ccaggggatg agccggcaac acagccacca
     4501 acaaaagaag aataagatga gaacacactt actttctaaa accaatacaa tgaccccgga
     4561 gcctccttcg cttacgagat ctggtatcgt gataactaag gacgggacca ttgtaccggc
     4621 caacggtcac ctttcccagg agccatcgcc ggcctctgcc ggcatcccgg aaacgaacct
     4681 ctttaacctg ctgccgggcc gccaccgcag cttcctgaaa acgatgctga accgggccga
     4741 ccgctgcaaa tcgtcacagg tgtccatgag attcgataac agccggccgg gtgatagtga
     4801 atgggaggtc ataaaactat tcgctgccgg ccctgccgca ccggtctatc tctgcctcgt
     4861 cccgctacac gatgccgccg cgttgggtcc aagcgacgct agccagtcga tcaagtgcaa
     4921 ggcgctcgat gagcgggcgg cttccggtat tatagtagaa gatgagaatg gaggcattct
     4981 ttgggcgaat caaaaatccg cccggttact cggcgttccg ccgaacgagc tttttacacc
     5041 acactttctt gaaaaactat ggaaaaccta ccatcatgtc catggctcag taccttttga
     5101 agcaaattct ccctctggat ccagacggcc agggttgttg ccgtcaagcg caaccctggt
     5161 tattaaacaa gtagatcgca aatataaaac cttgtccttt gagctggaag aaatatcccg
     5221 gcgaaacttt tctcccagcc ttgtcttagt caggataaaa gagattatgg ccggggaggc
     5281 cgaatcatca cccgtcattt ccaatgactc cgactgggtt tggatgcgac gtgagtatat
     5341 tgagaagacc atcgccaagg cgaaggaggc ggaaaggatc aggatcggtc atgaattaca
     5401 tgacaatatc aaccagatgc tggccgtgat cggagtgtac ctgagcctgt tgcatccggc
     5461 tgatcccgac gagaaggatc tccttggtaa atcagggcat atcctcaaga tggccatcga
     5521 agagatccgg tgtctttccc atggcctggt gaaggattcc accctcaggg aaggcttcat
     5581 tgctgccgta acggcattgg tggatgattt ccggcaaacc cgaatgttca atatttcctt
     5641 tacattcaat aaccaggagg tggaatcgat ggatcagtcg aaaaaagtag ccctttaccg
     5701 catcctacag gagcacctga acaacatcgt caaatatagc caggcaaaga atgtacagat
     5761 ctgcctggat accgatcaaa aaggcgtgcg tctcaccata gaagacgatg gggtaggatt
     5821 taacaagctg acggtgcaaa agggcatcgg cctcaccgga atttatgaaa aggccgagct
     5881 ttgcaatggt tccgttgaat tgcagacgtc tcccgggcat ggatgtatct taaaagttac
     5941 cgccatgcaa ggtgtttccg aaaattgatt cccggtttat caagactttc aagttattac
     6001 attaaattcg ggatagttct tagtgaggca aatgcctgga ctgatggttg gaaaaatgcc
     6061 gtagtcgata tttcatcgcg gggacctatt ggggacctgg aaaaggtcaa cgtcctgata
     6121 cccgccacag ataagaactt tacagggtag attcgacacc ctccctctcc gccaacaaaa
     6181 gaagaactaa acctctggct ttgccggggg aattttctac acggggatgc aaaggagcga
     6241 agcgaccttt acgaaggtac tcgaaagctg tttcatagcc tcacattgtt cgttttgatc
     6301 aacaatttgc ctaacacacg tcagccaaac ggaccttcct tgcaggcttt agaaattaag
     6361 taatttagca aaagcctcgc aatcatcaac ttcgattgaa aatggcgacc aatgaactgg
     6421 aaaaagagac cgaaaatcga actacggatc agaaggtttc aggtttgaat cctgacagag
     6481 tcactgatta tcaaagagtt aaaaggggtt aatcttgtaa aaaagattgg tccctttttc
     6541 tttttctacc gtctccagct gatctttttt gataatattt tttggatcag tgaaacgggg
     6601 aacttttcgt ttctgcata
//
LOCUS       DATGFG010000740         4358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1276538, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000740 DATGFG010000000
VERSION     DATGFG010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276538"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VKQ52_13845"
     CDS             complement(<1..688)
                     /locus_tag="VKQ52_13845"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88331.1"
                     /translation="MAKTAKSGTASSSILTSKSWEFLKKYIDNPSPVGFESSGQRLWL
                     DYLKPYVDSHFTDAYGTAVGVVNPAAPFKVVIEAHADEISWFVNYINAEGLIYLKRNG
                     GVDHQIAPGMRVFIHGKKGQVPAVFGWPAIHTRIANTDSKEPQPKVDSLFLDTGGRNK
                     KEVEELGVHIGSVVTYQDGYAEMGYDYLVGRAFDNRVGGFMIAEVARLLRENRKKLPY
                     GLYVVNAVQEE"
     gene            complement(720..1559)
                     /locus_tag="VKQ52_13850"
     CDS             complement(720..1559)
                     /locus_tag="VKQ52_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HLZ88332.1"
                     /translation="MFRKIVDYFVFSSLYIALCAVLMMWQTTWLLLGTAPPGRLLGFV
                     FFSTICSYNFHWYLTPRSTSPSRRVQWTHKHKTVHFVLYLTGVVGAAVYFFYLAHYFI
                     ALCVGAFLTFLYSAPKLPQRAFRQLREIAVGKTLFLAFVWVYVTTVLPLIVAGAHWNF
                     SFVLFTAGRFFLIYGICIIFDYRDREDDKRHGIRSLITLLDEKGIDRLFGLSMILFFL
                     SNFALAFYHFPAWYIFLLVIPGIITAALYRLSKRNFSDDLYYIVLDGLMMFSGLLMFI
                     FRI"
     gene            complement(1589..2041)
                     /locus_tag="VKQ52_13855"
     CDS             complement(1589..2041)
                     /locus_tag="VKQ52_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88333.1"
                     /translation="MKPGYFLVSVLLLVLIGIGCSKSNSKSKPKITLESINTVVNHND
                     SMRAMFKISNASGLSNGTFVAIRNRINQVPFPVTDSAGVDTFPVAIPNFNGSASGEMR
                     FALPVQGFLSESANLHMNDTLIYKFFVLTTGNVSSDTISSPKIVILNP"
     gene            2127..3098
                     /locus_tag="VKQ52_13860"
     CDS             2127..3098
                     /locus_tag="VKQ52_13860"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88334.1"
                     /translation="MHLDFFNNSNPADLVPRALELFRFQYANNPLYRQYTETLGVDPA
                     GVTRLEEIPFLPVSFFKTDRVKTTDFVPETVFTSSGTTGVVSSRHEIKDLDLYKRSFR
                     TAFDQFYGPAGDWCIIGLLPSYLERTSSSLVVMVDDLIRRSGHPRSGFYLYEHEALYR
                     VLQELEARGQKTLLIGVTFALLDFAEKYSLSLQHTVIMETGGMKGRRRELTRAELHAF
                     LAERLGVASVHAEYGMTELLSQAYSPGQGLFTCPPWMRMLVRTEDDPLEVRTAGEGIL
                     NIIDLANCWSCAFLATDDVGRIYPDGRFAVSGRVDNSDIRGCSLLVA"
     gene            complement(3128..>4358)
                     /locus_tag="VKQ52_13865"
     CDS             complement(3128..>4358)
                     /locus_tag="VKQ52_13865"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HLZ88335.1"
                     /translation="AVSETVARDAKEQGILLNVADTPDLCDFYLSSVVRKGNLKIAIS
                     TNGKSPTIAKRLKEELGGMIPDEMESVLNNMQTIRQRLNGDFPEKVRKLNELTKVLVA
                     KQATPAAQAKQSRWQQVVKWCLFAFFFMIVGHGLLSWVPVAGIVEFFRNLPQRFDVNG
                     FLIMTLAGFLAQMVDGSLGLGYGTISTTFLLAYGVSPAIVSSRVHSARVFSSGVSGYS
                     HHRFGNINKRLFRAIVIPGIFGAILGAAMAYFGQGYAKFVRIPLSLYTIYLGYYILSK
                     AFARHNPKDKVKRAGWLASAGGFTDAFAGGGWGTLVTSTLISKRRNPRYVIGSVCLAE
                     FFVVLASSITFFILLQNIPLLDVAGMILGGVVAAPIAARLVGKLPVKTMFIAVGALVM
                     LTSCSTLWKAIQQVIHH"
BASE COUNT         1012 a         1172 c         1277 g          897 t
ORIGIN      
        1 cttcctcctg cacggcattt accacataca ggccataggg cagctttttc ctgttctccc
       61 ggagaagccg ggccacctcg gcgatcatga acccgccgac gcgattgtcg aatgcccggc
      121 cgacgagata atcatagccc atttcggcat agccatcctg ataggtgacg acggagccga
      181 tgtgcacccc caattcttcc acttccttct tgttacggcc ccccgtatcg aggaagagac
      241 tgtccacttt tggctgaggc tcctttgagt cggtattggc gatccgggta tggatggccg
      301 gccagccgaa gacggccgga acctggcctt ttttcccatg gataaagacc cgcatgccgg
      361 gggctatctg gtggtccacg ccgccgttgc gcttgagata gatcaggcct tccgcgttga
      421 tatagttgac aaaccagctg atctcgtcgg catgggcctc gatcaccact ttgaagggcg
      481 cagccgggtt gacgacgccc acggccgttc cgtacgcatc ggtaaaatga ctgtccacat
      541 aaggtttcag gtagtcgagc cagagccgct gaccactgct ttcaaaacct acaggtgatg
      601 gattgtcgat atatttcttc aggaattccc aggacttgct ggtcaggatg gaggaggatg
      661 ccgttccgct tttcgcagtt tttgccatgt agtaaataac ttatgagcag caaatgtact
      721 taaatcctaa aaatgaacat cagcagcccg gaaaacatca tcagcccgtc caggacgata
      781 taatacaggt cgtcggagaa attgcgtttg ctcagccggt acagggcggc ggtaatgata
      841 ccggggatca ccagcagaaa gatataccag gccggaaaat ggtagaaggc aagggcgaaa
      901 ttgctgagga agaagaggat catcgatagg ccaaacagcc ggtcgatgcc tttttcgtcg
      961 agcagggtga tcaggctccg gataccatgg cgtttgtcgt cctcccggtc gcggtaatcg
     1021 aagatgatac agataccata gatgaggaag aaacggccgg ccgtgaacag gacaaaggag
     1081 aaattccaat gggcgccggc cacgataagg ggaagcacgg tggtcacata gacccagaca
     1141 aaggccagga agagcgtctt gccgacggcg atctcccgga gttgccggaa ggcccgctgg
     1201 ggcagttttg gggcggaata gaggaaggtg agaaaagccc cgacgcaaag ggcgatgaag
     1261 taatgggcca ggtaaaagaa atagacggcc gcgccgacaa cgccggtgag atagaggacg
     1321 aagtgtacgg tcttatgttt gtgggtccac tgtacccggc gggaagggct ggtcgaccgc
     1381 ggggttaaat accaatggaa gttgtagctg caaatagtcg agaagaaaac aaatcccagg
     1441 agccgccccg gaggcgcggt ccccagcaac agccatgtcg tttgccacat catcagtaca
     1501 gcgcataaag caatatagag gctgctgaat acaaaataat cgacgatttt tctaaacacg
     1561 ctgatcccaa ttggttaacg ggccgaagtt acggatttag gattacgatc ttgggagaag
     1621 atatggtatc gctggagaca ttgccggtgg tgagcacgaa gaatttgtag atgagcgtat
     1681 cgttcatatg aaggttggcg gactcgctca ggaatccctg taccggcagg gcaaaccgca
     1741 tctccccgct ggcactgccg ttgaaattag ggatggccac cgggaacgta tccacgcccg
     1801 ccgaatcggt caccgggaag ggaacctggt tgatccggtt gcgaatggct acaaaggttc
     1861 cgttgctgag gccgctggca ttgctgatct tgaacatcgc ccgcatggaa tcgttgtgat
     1921 tcacgaccgt attgatggat tcgagggtga tcttgggttt gcttttggaa ttgctcttgc
     1981 tgcagcctat tccgataaga acgagcagta atacggatac caggaaatag ccaggcttca
     2041 tgttgaggga tttaaatcga gccgctggaa gctgctcctg cctacaaacc tacctaattt
     2101 taactaaaag gggctaattt tgtggagtgc atttagactt ttttaacaat tcgaatccgg
     2161 cggatcttgt cccccgggcc ctcgaacttt tccggttcca gtacgccaac aatccccttt
     2221 acaggcaata taccgagaca cttggggtag accctgccgg cgttacccgg ctggaggaga
     2281 tccccttcct gccggtaagc ttcttcaaga ccgatcgggt aaagaccacc gattttgttc
     2341 ccgagaccgt attcaccagc agcgggacga cgggggtggt gagcagccgt catgaaataa
     2401 aggacctcga tctgtacaaa cggagcttcc ggacggcttt tgaccaattc tacggcccgg
     2461 ccggggactg gtgtatcatc gggctcctgc cttcctacct ggaaaggacc agtagttctc
     2521 ttgtcgtcat ggtcgatgac ctcatcagac gcagcgggca cccccggagc gggttctatc
     2581 tgtatgagca cgaagcgcta tacagggtgt tgcaggaact ggaggccagg ggacaaaaga
     2641 ccctgctcat cggcgtgacc ttcgccctgc tggattttgc cgaaaaatat tccctgtccc
     2701 tgcagcatac cgtcatcatg gaaacagggg gaatgaaggg ccggcggcgg gagctcaccc
     2761 gggcggaatt gcacgccttc ctggccgaac ggctgggagt agcgagcgtc catgcggaat
     2821 atggaatgac cgagttactg tctcaggcct attcccccgg acaagggctt ttcacctgtc
     2881 cgccctggat gcggatgctc gtgcgcaccg aggacgatcc gctggaagtg aggaccgccg
     2941 gggaggggat actcaatatc atcgatctgg ccaattgctg gtcctgcgct tttcttgcca
     3001 ccgatgacgt cggccgcata taccccgatg gccgcttcgc ggtttcgggc cgggtggaca
     3061 acagcgatat ccggggatgc agcctgttgg tggcctagat acggctcccg ttgtcagcgc
     3121 cgcaatatca gtgatggatc acctgctgga tggccttcca cagggtgctg caactggtca
     3181 gcatgaccag cgcgccgacg gcgatgaaca tggtctttac cggcagctta cccaccagcc
     3241 gggccgcgat gggggcggcg accactccgc ccaggatcat cccggcgaca tcaagcagcg
     3301 ggatattctg caacaggata aaaaaggtga tggaactggc aagcacgaca aagaattccg
     3361 caagacagac cgaaccgatg acataacgcg ggttccgccg cttcgagata agggtgctgg
     3421 tcacgagcgt gccccagccg ccgcccgcaa aggcgtccgt aaacccgccc gccgaagcca
     3481 gccagcccgc ccgtttgacc ttgtccttgg ggttgtgccg cgcgaacgcc ttacttagaa
     3541 tataatagcc gaggtagatg gtgtataacg aaagggggat gcggacgaat ttcgcatatc
     3601 cctgaccgaa ataggccatg gcggcgccca ggatggcgcc aaagatgccg gggatcacaa
     3661 tggcccggaa caaccgcttg ttgatattgc caaaacggtg atggctgtat cccgatacgc
     3721 cgctggaaaa gacccgcgca gagtgcaccc ggctgctcac gatggcgggg ctgacgccat
     3781 aggccagcag gaaggtcgtg gagatggtgc catagccaag acccagcgat ccgtcgacca
     3841 tctgcgccag gaagccggca agggtcatga tcaggaagcc gttcacatcg aaccgttgag
     3901 gcaggttccg gaagaattcc acgatgccgg ccaccggaac ccaggagagc agtccgtgac
     3961 cgacgatcat aaagaaaaag gcgaagagac accatttcac cacctgctgc cagcggctct
     4021 gctttgcctg ggcagccggg gtcgcctgct tggccacaag aacctttgtc agttcattga
     4081 gcttgcggac cttctcaggg aaatccccgt tgagccgctg ccggatggtc tgcatattgt
     4141 tcagcacgct ttccatctcg tcggggatca tcccgcccaa ttcttctttt aaccgtttgg
     4201 cgatggtcgg cgatttgccg ttcgtggaga tcgcgatctt caggttgccc ttccggacga
     4261 cggagctgag gtaaaaatcg cagagatccg gggtgtcggc tacattgaga agaatgccct
     4321 gttcttttgc gtcgcgggcc accgtttcgc tgacggcc
//
LOCUS       DATGFG010000741         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_686322, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000741 DATGFG010000000
VERSION     DATGFG010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686322"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VKQ52_13870"
     CDS             complement(<1..958)
                     /locus_tag="VKQ52_13870"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLZ88336.1"
                     /translation="MSRTPGSLGFFRSTLLLTLLAFGCGHPQQGGSSPATEKPAADTG
                     SAAATTSGHGHTVLQYAHGFRIDDHGAYREVSILNHTGGRIDTLHYLLVEEGVTPPAG
                     RPGIPVITTPVKTLAVLSSMHVALAGFAGVADRITGLGNFRYINSPIVREGIRTGKVK
                     QIGIDAGINNELVISLHPGVVISMTNPDAAFGQFKTLTEAGIPVLPDADWLETTPLGR
                     AEWVKLVGALVDREDYVAKKFDSVAQAYLRLAAIGRSAKKKPSVILGMPFKGVWYTPA
                     GENYMAQFLRDAGAGYHWSDTKGISSLSLNVESVIPIALQADY"
     regulatory      complement(1011..1209)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1286..2026
                     /locus_tag="VKQ52_13875"
     CDS             1286..2026
                     /locus_tag="VKQ52_13875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ88337.1"
                     /translation="MSFIRKANSFVKDFLLVSRVGILLTPFRFAFKFLYNFSMLSSWV
                     NRQSGKIPFSDFYKPFRKYTNRVRLHDHVVSSQGLDKKPIHYLEFGVAGGTSFAWWIK
                     ANGHPDSRFFGFDTFEGLPEDWHFFKKGAFSYDIPFMDDARGTFIKGLFQDTVYTFLD
                     NYRSRIDTPEATRVLHLDADLYSSTLFALTMMAPFLRNGDILLFDEFNIPNHEFAAWS
                     DFIRSYYIKYEVIGCVNNFYQTAIRIIK"
     gene            complement(2027..>2229)
                     /locus_tag="VKQ52_13880"
     CDS             complement(2027..>2229)
                     /locus_tag="VKQ52_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88338.1"
                     /translation="YSLLGGRIGYRRILGKLRFDIYSVVDNAGNTKYSLGNDFNAAVG
                     RYYNVAPGRNYFAGVSVNYALQ"
BASE COUNT          455 a          600 c          641 g          533 t
ORIGIN      
        1 agtagtcggc ctgcagggcg atggggataa cggactcgac attcagcgac aggctgctga
       61 tgccctttgt gtcggaccag tggtatccgg cgccggcgtc gcgcaggaat tgcgccatgt
      121 agttctctcc tgccggggta taccagacgc ctttgaacgg catccccagg atgaccgacg
      181 gcttcttctt cgcgcttctg ccgatggcgg ccaggcggag ataggcctgc gccacgctat
      241 cgaatttttt tgcgacgtag tcttccctgt cgacgagggc gcccaccagc tttacccatt
      301 cggcgcggcc cagcggtgtc gtctccagcc agtccgcatc ggggaggacc gggatgccgg
      361 cctcggtgag ggtcttgaat tgcccgaatg ccgcgtcggg attggtcatc gagatgacga
      421 cgccgggatg gagggagatg accagctcgt tgttgatgcc ggcatcgatg ccgatctgtt
      481 ttaccttacc cgtccggata ccttcccgca cgatggggga gttgatataa cggaaattac
      541 cgagcccggt gatacgatcg gctacacccg caaaccccgc cagggcgaca tgcatcgatg
      601 aaagaacggc cagggttttc accggggtgg tgatcaccgg tataccgggc cggccggccg
      661 gcggggtaac gccctcttcc accagcaggt aatgcaaggt atcgatcctg ccaccggtgt
      721 ggttgaggat gcttacctcc cggtaggcgc catgatcgtc gatccgaaag ccgtgggcat
      781 attggaggac ggtgtggcca tggccgcttg tcgtggcagc ggcggagccg gtgtcggcgg
      841 cgggtttttc tgtcgccggg gaggagccgc cctgctgcgg atgcccgcag ccgaaggcaa
      901 ggagggtcag cagcagggta gaacgaaaga agcccaggct tccgggggtt ctggacatag
      961 tgtcaattgc ttttatgagt agaatacagc ctgcttttta cccgaaagcc agtcttgtcg
     1021 atgataccgg caggtcttct ggcttgtacc tgatcccgcg tgccttcccg ttccgccagg
     1081 ggcggaacag tggtgaggag tggggctgga tcacctgtat ggtacttaca gctacgggca
     1141 tagcgccgga attgcaccgg acttcccttt tcgtccgcgc gacgcggaac cgatatcgcc
     1201 gcgaagctac aaaaaaggcc ggctaatcgt accgtttttc gatctatctt tagcccctta
     1261 atattgtgaa aattttatat ttcatatgag tttcattcga aaagccaatt cctttgtaaa
     1321 ggacttcttg ctggtttcac gggttggtat tcttttaact ccctttcgct tcgccttcaa
     1381 attcctctat aatttctcga tgctttcttc ctgggtgaac aggcaaagcg gaaagatccc
     1441 gttttcggat ttttataaac ctttccggaa gtatacgaac agggtcagac tccatgacca
     1501 tgtcgtcagc agccagggat tggataaaaa accgatccat tacctggaat tcggggtggc
     1561 gggcggaacc tccttcgcgt ggtggataaa ggctaacggg catccggatt cgcgtttctt
     1621 tgggttcgat actttcgagg gacttcccga ggactggcat ttctttaaga agggggcgtt
     1681 cagctatgat atccctttta tggatgatgc acgcggaacc tttatcaagg gcctcttcca
     1741 ggataccgtg tatacctttc tcgacaacta tcgcagccgg atcgatacgc cggaagccac
     1801 gagggtgttg catctcgatg ccgacctcta ttcttccact ttatttgcgc tgacgatgat
     1861 ggcgccgttc ctgcgcaacg gggatatcct gctcttcgac gaattcaata tccccaacca
     1921 tgagttcgcg gcctggagtg atttcatccg cagctattat atcaaatacg aggtgatcgg
     1981 ttgtgtgaac aatttctacc agacggcgat caggatcatc aaataattac tgtaaggcat
     2041 agttgacgga taccccggca aagtagttcc tgcccggggc cacattgtaa taccggccga
     2101 cggccgcgtt gaagtcattc cccaggctgt atttcgtatt cccggcatta tcgacgacgg
     2161 aatagatgtc aaaccgcagc ttcccgagga tgcgccggta accgatgcgc ccgcccagca
     2221 ggctatagg
//
LOCUS       DATGFG010000742         5709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_272571, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000742 DATGFG010000000
VERSION     DATGFG010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272571"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..534
                     /gene="rplL"
                     /locus_tag="VKQ52_13885"
     CDS             154..534
                     /gene="rplL"
                     /locus_tag="VKQ52_13885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HLZ88339.1"
                     /translation="MADLKAFAEQLVGLTVKEVNELAKILKEEYGIEPAAAAVAVAAG
                     PAAGGGAAAAEEKSSFNVILKAAGANKLQVVKVVKDLTGLGLKEAKDLVDGAPKPVKE
                     GISKADAEALLAKLKESGADVEIQ"
     gene            829..4632
                     /gene="rpoB"
                     /locus_tag="VKQ52_13890"
     CDS             829..4632
                     /gene="rpoB"
                     /locus_tag="VKQ52_13890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLZ88340.1"
                     /translation="MSSTKLQSRINFGKIKHLAETPDLLEVQIQSFKEFFQLETTPDK
                     RNIEGLFKVFKENFPITDTRNIFMLEFLDYFIDPPRYTIEECMERGLTYAVPLKAKLR
                     LSCNDEEHVDFQTIVQDVFLGNIPYMTPRGTFVINGAERVVVSQLHRSPGVFFGQSIH
                     PNGTKIYSARVIPFKGAWMEFATDINNVMYAYIDRKKKFPVTTLLRSIGYETDKDILE
                     LFGMADEVKVDKKSLQKYIGKKLAARVLRTWVEDFVDEDTGEVVSIERNEIVLERDTI
                     LDEEAIDMVVEMDVKSIFIQKEDVGGDYAIIYNTLNKDTSNSELEAVQHIYRQLRGAD
                     APDDETARGIIDKLFFSDKRYDLGEVGRYKINRKLNLNQPLDQKTLTKEDMIEIIKYL
                     VRLTNAKAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNEVFTPV
                     DLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRERAGFEVR
                     DVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINEMGFIETPYRKVNDGKVDLKKLTY
                     LSAEEEDVAKIAQANIALDEKGNFVEEKIVSRQTGDFPILDRHEVEFMDVAPNQIVGL
                     SASLIPFLEHDDANRALMGSNMQRQAVPLIRPEMPIVGTGLEAKAARDARIQIHAEGD
                     GVVEFVDANEIHVRYDRSEGQKLVSFEEDLKVYRLTKFIKTNQETCINLKPAVKKGQK
                     VKEGYFLTEGYAVQDGELALGRNLKVAFMPWKGYNFEDAIVISEKVVKEDLFTSVHIS
                     EYELEVRDTKLGEEELTPDIPNVSEEATKDLDENGIIRVGAQIKEGDILIGKITPKGE
                     SDPTPEEKLLRAIFGDKAGDAKDASLKAPSGTEGVVISKKLFQRAKKDKNAKVREKAA
                     LEKIEKVHEKNTSDLMDVLLGKLTVLLKEHVSAGVNNNFGEVQIGKGAKFTAKNLSGI
                     DYLNVNPLGWTGEAKTDDSINILLHNYSIKYNEELGRYKREKFNISIGDELPAGVLKL
                     AKVYLAVKRKLKVGDKMAGRHGNKGIVAKIVRAEDMPFLADGTPVDVVLNPLGVPSRM
                     NLGQIYETVLGWAGEKLGVKFATPIFDGATVEEIADHIEKAGLPSFGHTYLYDGETGE
                     RFDQKATVGIIYIIKLHHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAYGASNILQELLTLKSDDIIGRAKTYESIVKGDNIPRAGIPESFNVLVHELRGLGL
                     DLKFD"
     gene            4857..>5709
                     /locus_tag="VKQ52_13895"
     CDS             4857..>5709
                     /locus_tag="VKQ52_13895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HLZ88341.1"
                     /translation="MLQYSIYPAFFRTMLLCMGFALYFLPSGAQPARPLFSPAAVDAA
                     RLTSLHDNYQLQYKEESERLPSQFRKDYQECYQERWKNIEEKFTKQEIYTSVEAQAYL
                     DRFVAEIVKSNPFVSGHPFHCYFSRSYIPNASYIGEGIILFNMGLFDRLSDESQAAFI
                     LCHEIAHCYLQHPEKSIGKYVATLNSEEVQGQLRKIKGAEYRKREQLQSLVRGLTFDS
                     RRHSRDHESEADSLAVVLMRHTRFDVAGALTALALLDGIDKNPFDTEGGLRWAFDAKE
                     YPFRKKWI"
BASE COUNT         1415 a         1362 c         1669 g         1263 t
ORIGIN      
        1 gtttgaggca ttcaaaattt ataatttaac attcaacata attttaacat tcaacataaa
       61 gataaaaccc aggcctgggg gtcaatcaag gcaaaatttt tttaaactat ccgccggagc
      121 attaagccgt gaaggcggga aaaatttaac attatggcag acttaaaagc atttgctgaa
      181 cagttagtag gactgactgt aaaagaagtt aacgaattag ctaagatcct gaaggaagaa
      241 tacggtatcg aacctgcagc tgctgctgta gccgttgctg ccggtcctgc tgctggtggt
      301 ggcgctgctg ctgctgaaga gaagagctcc ttcaatgtta tcctgaaagc cgccggcgct
      361 aacaagctcc aggtagtgaa ggtagttaag gacctgaccg gtctgggcct gaaagaagcc
      421 aaggacctcg ttgacggtgc tcccaagccc gttaaggaag gtatcagcaa ggctgacgcc
      481 gaagcgctgc ttgccaagct gaaggagtcc ggtgctgatg tggagatcca gtaagcgcgg
      541 cgcctgtcca agctttaatg gcaccacgta ggcatggaaa gtacagcaat tagtaagata
      601 aggacgagag gcgtaaggaa tccgattgat tttatgaccc cggcgagagt cggggttatt
      661 tttttgcggg gaagtaaaaa agtactattt ttgcggtccg tttcccttgg ctaagttact
      721 cattgataat cgtttacgca tttaagtaaa tgaaagtcaa tgggttagat ttattgctgt
      781 gcagacgagt cggacgttcg aaacataaaa aaaccggttt taaacaatat gtcttcaaca
      841 aaattgcaaa gcaggatcaa tttcggtaaa atcaagcatc ttgccgaaac tcctgacctt
      901 ctggaagtac agatccaatc tttcaaagaa ttttttcagc ttgagactac cccggacaaa
      961 cgaaatatcg aaggtttgtt caaggttttt aaagaaaatt tcccgatcac cgatacgcgg
     1021 aacattttca tgctggaatt tttggattac ttcatcgatc ccccccgtta cacgatcgaa
     1081 gagtgtatgg agcgcggcct gacctatgcg gttcccctga aggcgaagtt gcgtttgagt
     1141 tgtaacgatg aggagcatgt ggactttcag acgatcgtgc aggatgtgtt ccttggtaat
     1201 atcccctaca tgacgccccg cgggacgttt gtgatcaacg gcgcggagcg tgtcgttgtt
     1261 tcacagctgc accgtagccc gggcgtattc tttggccagt ccattcaccc gaacggaacg
     1321 aagatctatt ccgcgcgggt gatccctttc aagggggcgt ggatggagtt cgctaccgac
     1381 atcaacaatg tgatgtatgc gtacatcgac cggaagaaga aattcccggt tacgaccctg
     1441 ttgcgcagta tcggttatga gaccgacaaa gatatcctgg agctgttcgg gatggctgac
     1501 gaggtgaagg tggacaagaa gtccctgcag aaatatatcg gcaagaagct ggctgcccgt
     1561 gtactccgta cctgggtgga ggactttgtc gatgaggata cgggtgaggt tgtctccatc
     1621 gagcgtaacg agatcgtgct cgagcgggat accatcctcg acgaagaggc tatcgatatg
     1681 gtggtcgaaa tggatgtgaa gagcatcttt atccagaagg aagatgtcgg cggcgactat
     1741 gcgatcattt acaatacgct caataaagat acctccaact ccgagctgga agccgttcag
     1801 catatctacc gccagctgcg cggtgctgac gctcccgatg acgagaccgc gagggggatc
     1861 atcgataaat tattctttag cgacaagcgc tatgatcttg gtgaagtggg tcgttataag
     1921 ataaaccgta agctgaacct gaaccagccg ctggatcaga agacgctgac gaaggaagac
     1981 atgatcgaga tcatcaagta tctcgttcgg ttgaccaatg ccaaggccga gatcgacgat
     2041 atcgaccacc tgagcaatcg ccgggtgcgt acggtgggtg agcagttgta tgctcagttc
     2101 ggcgtgggtc ttgcgcgtat ggcgcgtacg atccgggagc ggatgaacgt acgggacaat
     2161 gaagtcttca cacctgtgga cctgatcaat gcgaggacct tgtcttccgt gatcaacagt
     2221 ttcttcggta cctcccagtt gtcgcagttc ctcgaccaga ccaacccgtt gagcgagatc
     2281 acgcacaagc gtcgtatctc cgcactcgga cctggcggtc tgtcccgtga gcgggcgggc
     2341 ttcgaagtcc gggacgtaca ctactcgcac tatggccggc tgtgtacgat cgagacgcct
     2401 gaaggaccga atatcgggct tatctccacg ctttgcgtac atgcgaagat caatgagatg
     2461 ggctttatcg aaacgcctta ccgcaaggta aacgatggca aggtcgatct gaagaagctg
     2521 acctatctca gcgccgagga agaagatgtc gccaagatcg cacaggctaa tatagcgttg
     2581 gatgaaaagg gcaacttcgt tgaagagaag atcgtttccc gccagaccgg tgacttcccc
     2641 atcctggaca ggcatgaggt ggagtttatg gacgttgctc ccaaccagat cgtgggtctc
     2701 agcgcctcgc tgatcccctt cctggaacat gatgacgcca accgtgcgct gatgggatcg
     2761 aacatgcaac gtcaggccgt cccgctgatc cgtcccgaga tgccgatcgt cggtaccggg
     2821 ctggaagcaa aggctgcccg tgacgcgcgg atccagatcc acgccgaagg cgacggggtt
     2881 gtagagttcg tggatgccaa cgagatccat gtccgttatg accgtagtga gggacagaag
     2941 ctggtctctt ttgaagaaga cctgaaagta taccggctga caaagttcat caagaccaac
     3001 caggagacct gtatcaacct gaagcccgcc gttaagaagg gccagaaggt gaaagaaggc
     3061 tacttcctga ccgaagggta tgccgtacag gatggtgaac tggcccttgg ccgtaacctg
     3121 aaggtggcgt tcatgccctg gaagggttac aacttcgagg atgccatcgt gatcagcgag
     3181 aaggtggtga aggaagatct tttcacctcc gttcacatct ccgagtatga actggaagtt
     3241 cgtgatacca agctgggtga agaagaactg acgcccgata tccccaacgt ttccgaagag
     3301 gccaccaagg acctcgacga gaacgggatc atccgtgtcg gcgcccagat caaggaaggc
     3361 gatatcctga tcggtaagat cacgccgaag ggcgagagcg atccgacccc ggaagagaag
     3421 ctgctgcgcg ccatctttgg tgacaaggcc ggcgatgcga aggatgcttc cctgaaggcc
     3481 ccgagtggta ccgaaggggt ggttatctcc aagaagctgt tccaacgggc caagaaagac
     3541 aagaatgcca aggttcggga aaaggccgct ctcgagaaga tcgagaaggt acacgagaag
     3601 aatacttccg atctgatgga tgtattgctt ggcaaactga ccgtattgct gaaggagcat
     3661 gtatccgccg gggtgaacaa caatttcggc gaggtgcaga tcggcaaggg cgccaagttc
     3721 acggctaaga acctgtccgg tatcgactat ctcaacgtca acccgctggg ttggacgggc
     3781 gaggcgaaga ccgacgattc gatcaatatt ttgctgcaca actacagcat caaatacaac
     3841 gaggagcttg gccgttacaa acgcgagaag ttcaatatct ccatcggtga cgagttgccg
     3901 gcaggggtgt tgaaactggc gaaggtgtac ctggctgtta agcgtaagct gaaggtgggg
     3961 gataagatgg ccggccgcca tggaaacaag ggtattgtcg cgaagattgt aagagccgag
     4021 gacatgccgt tcctcgccga tggtacgccg gtggatgtag tattgaaccc gctgggtgta
     4081 ccgagccgta tgaacctggg ccagatctat gaaacggttc ttggctgggc cggcgagaag
     4141 ctgggtgtca agtttgccac gccgatcttc gacggggcaa cggtggaaga gattgccgac
     4201 cacatcgaga aggcgggtct gccgagcttt ggccacacct atctttatga tggggagacc
     4261 ggcgagcggt ttgaccagaa ggcgacggtc gggatcatct atatcatcaa gctgcaccac
     4321 atggtcgatg ataagatgca cgcccggagc atcgggcctt acagcctgat cacgcagcag
     4381 ccgctgggtg gtaaggcgca attcggtggc cagcgttttg gtgagatgga agtttgggcg
     4441 ctggaagcct atggcgcatc gaatatcctg caggagttgc tgaccctgaa gtccgatgat
     4501 atcattggac gtgcgaagac ctatgagtcg atcgtgaagg gagacaatat cccgagggcg
     4561 ggtattcccg aatccttcaa cgtgcttgtg catgaattga gggggttggg cctggacctg
     4621 aagttcgatt aatggggcgc ggcgcaaaca acaatggctc cgctaactga tgaataaata
     4681 cgaaaagcca ccttttgggg tggctttttc ttttgggagt gcgtttccgg ggcggcaccg
     4741 ggtcgcggca ggcacggcta tccggctatt cgacacggtt atgcctgttt ttgacgcgga
     4801 cttggattta tatgaaatcg gaactatatt cggccgtgac ctaagcccga aagtacatgc
     4861 tccaatattc tatctacccc gcgttcttcc gtacgatgct gctctgtatg gggtttgccc
     4921 tgtatttcct cccgtccggg gcgcagccgg cacggccgct cttttcaccc gcagcagtag
     4981 acgccgcccg gctgacgagc cttcacgaca attaccagct ccagtataaa gaggagtcgg
     5041 agcgtcttcc ttcccaattc aggaaagatt accaggaatg ctaccaggag cggtggaaga
     5101 acatcgagga gaaattcacc aaacaggaga tctatacgtc tgtggaggcg caggcctacc
     5161 tggaccggtt tgttgcggag atagtcaaaa gcaatccctt tgtcagcggg catcccttcc
     5221 attgttattt ttcccggagc tatattccca acgccagcta tatcggcgaa gggatcatcc
     5281 ttttcaatat ggggctgttc gaccggcttt ccgatgagag ccaagcggct tttatccttt
     5341 gtcatgagat cgcccactgc tatctgcagc atcccgagaa gagtatcggg aagtatgtgg
     5401 cgacgctgaa ttccgaggag gtgcaggggc agctccggaa gataaagggg gcggaatacc
     5461 ggaaacggga acagttgcaa tcgctggtgc gggggctgac ctttgacagc cgccggcata
     5521 gccgggatca tgagtccgag gccgacagcc tggcggtggt gttgatgcgg catacccgtt
     5581 tcgatgtggc gggggcgctg acggcgctgg cgttgctgga cgggatcgat aagaatccct
     5641 tcgatacgga gggtgggctg cggtgggcct ttgatgcgaa ggagtatccg ttccgcaaga
     5701 aatggatcg
//
LOCUS       DATGFG010000743         3607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1501734, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000743 DATGFG010000000
VERSION     DATGFG010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501734"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VKQ52_13900"
     CDS             complement(<1..202)
                     /locus_tag="VKQ52_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88342.1"
                     /translation="MSGNSYNRSYILGLSFISALGGYLFGFDFAVISGALPFLRDQFG
                     LDAYWEGLVTASLAVGAAAGCLV"
     gene            complement(209..1219)
                     /locus_tag="VKQ52_13905"
     CDS             complement(209..1219)
                     /locus_tag="VKQ52_13905"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HLZ88343.1"
                     /translation="MLTTELSQFMLCKGSIRVGIINLGAAITLLEAPDRSGRVKNIVA
                     GFSTPDEYWNNDHYFGCLVGRYANRIGGGLFQLDGRPVQLSVNNDGNHLHGGWEGFHK
                     QLWRLRTRIEKEQESGIVLEYTSPDGQEGYPGNCRVQVTYLLDIAGRLSIDYQATTDA
                     ATPVNLTNHSYFNLSGFDNPVVDDHLLQVFAGRYTEKNTRNLPTGRLLPVDGTPLDFT
                     HPRRIGDRFLHLPTDNGYDHNYVLDDANASAPAAELYDPVSGRQLKVFTDRPAIQVYT
                     ANWWDGSIVGRQGVPYSKHGAVALETQAYPDSPNQPSFPDTILRPGGVYRTTTIFELT
                     TK"
     gene            complement(1213..>3607)
                     /locus_tag="VKQ52_13910"
     CDS             complement(1213..>3607)
                     /locus_tag="VKQ52_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ88344.1"
                     /translation="RNLTDEDGPYIELMTGVFTDNQPDFSWLQPNEEKSFEQYFMPYT
                     LVGQVKNATKEAMLNLQWEGDTLHVKVYTTSDYPGARVLLLNKGQVVKEWTRRISPQQ
                     VFEETHLPAEKGSQGDWALVVTDAQNQVLVAWQDSDPGTEKRTIPAPATAAPAPADIA
                     QVEDLYLNGLHLEQYRHATYNPIDYYAEGLRRSPEDIRCNNAMGLLLLRRGQFEAAEP
                     HFRRAIASLTRRNPNPYDGEPYYNLGWSLKLQGRLEEAFDAFYKATWNDAWQHSGFLS
                     LARIATAQHRYKEALELVERSLVRNYHSPVARHLKAVLLRKTQKAAEPYIMDSLTLDP
                     FNFGCLFEQGSDQFDIRMRGSLNNYLELALDYAQAGLYEDADTVLQRCPANSPLLGYY
                     RGWFASLSGQKEAAIAHYRTAAKTDPACCFPNKIEEVLILRDAQKLNPADARAPYYLG
                     NLWYDKRQYTDAVACWEDAARLDPTFPTVLRNLALAYYNKLNKKEAALTLLEKAFHLD
                     TADARVLMELDQLYKIMGRPFEERLRFLEQYPDLIHQRDDLFLERITLCNQLKEYRTA
                     QQLLASRKFHPWEGGEGKVVGQYLLCHLELAKQAIATGHWQEALQLLASTGRYPENLG
                     EGKLYGTQENDRFYLQGLVYEKLGQPENARENFHAATVGISQPAQAIYYNDPQPDKIV
                     YQALSWFRLGQREKALRIFESFIRFGKEHLDDSITIDYFAVSLPDMLVFDQDINRRNR
                     IHCTYLIGLGHLGLGDEAAAHRYLHEVLRLDRNHQGAAVYLDMVPFFRQAIKQEPAC"
BASE COUNT          703 a          949 c         1107 g          848 t
ORIGIN      
        1 ccacaaggca gcctgccgca gcgcccacgg ctaggcttgc cgtcaccagt ccctcccagt
       61 aggcgtcgag cccgaactgg tcccgcagga agggcagcgc tccggaaata acggcaaaat
      121 caaaaccgaa gaggtatccg cccagtgccg agataaaaga taagccgagg atgtaggatc
      181 tattatagga gttaccggac atagatggtc atttggtggt gagttcaaaa atagttgttg
      241 tccggtagac gcctccgggt cttaggatcg tatccggaaa ggaaggctgg ttgggactat
      301 ccgggtaggc ctgggtctcc agggcaacgg cgccatgctt actatagggc accccctgcc
      361 gcccgacgat gctgccgtcc caccagttgg ccgtatacac ctggatggcc ggccgatcgg
      421 taaatacttt taattggcgc cctgacacag gatcgtagag ttcggcggcg ggcgccgatg
      481 cattggcatc gtcaagcaca tagttgtgat catatccatt gtccgtcggc agatggagga
      541 agcgatcccc gatccgccgc gggtgtgtaa agtcgagggg cgtgccatcg acaggcagga
      601 gccggcccgt aggtagattc ctggtgttct tctcggtgta acggccggca aacacctgca
      661 agagatgatc gtcgaccacg gggttatcga agccggaaag gttgaaatag gaatgattgg
      721 tgaggttcac cggcgttgcc gcatccgtgg tcgcctggta atcgatggac aatcgccctg
      781 cgatatccaa cagataggtg acctgcaccc ggcaattgcc cggataccct tcctgcccat
      841 cggggctcgt atattccagg acgatgccgc tttcctgctc tttctctatc cgggtccgga
      901 gccgccacaa ttgcttgtgg aaaccctccc accctccgtg gaggtgattg ccgtcattgt
      961 ttaccgacaa ctgtacgggc cgcccgtcga gttggaaaag tccgccgccg atgcggttgg
     1021 catatcgtcc caccaggcat ccaaaataat ggtcgttgtt ccaatattcg tcgggtgtcg
     1081 aaaagccggc cactatattc tttacccggc ccgaccggtc gggtgcttcc agcaaggtga
     1141 tggcagcccc gaggttgatg atgccgacac gaatggagcc cttgcagagc atgaattgcg
     1201 aaagctctgt cgtcagcatg cgggttcctg ttttatagct tgtcgaaaga aaggtaccat
     1261 atcgagatag acggccgcgc cctggtgatt cctatccagc cggagcactt cgtggagata
     1321 acggtgcgca gcggcctcgt cacccaatcc gaggtggccg agtccgatca ggtaggtgca
     1381 gtggatcctg ttgcgcctgt tgatatcctg gtcgaagacc agcatgtcgg gtaaggaaac
     1441 ggcaaaatag tcgatggtga tcgagtcgtc gaggtgttct ttgccgaacc ggataaagct
     1501 ttcgaagatg cgaagggcct tttcgcgttg acccaatcgg aaccaggaca gcgcctgata
     1561 gacgatcttg tcgggctggg ggtcgttgta atagatggcc tgggcgggct ggctgatgcc
     1621 tactgtggct gcgtggaaat tctcccgggc gttttcgggc tgtccgagtt tttcatagac
     1681 gagtccctga agatagaagc ggtcgttctc ctgcgtgccg tacagttttc cttcgccgag
     1741 attctccggg tagcgcccgg tggatgccag caattgcagc gcctcttgcc aatggccggt
     1801 ggcaatggcc tgtttggcta attcgaggtg gcagagcagg tattggccta ccacctttcc
     1861 ttctccgcct tcccagggat gaaatttccg tgacgccagc aactgttgcg cagtcctgta
     1921 ttctttcagc tggttgcaga gcgtgatgcg ttcgagaaaa agatcatccc gctggtggat
     1981 aagatcgggg tattgttcga gaaaacggag tctttcttcg aatggtcttc ccatgatctt
     2041 gtatagttgg tccagctcca tcagtacccg ggcatcggca gtatccagat gaaatgcttt
     2101 ttccagaagc gtaagcgccg cctccttttt gttgagctta ttgtagtagg ccagcgccag
     2161 gttgcgcaat acggtcggga aggtcgggtc caggcgtgcc gcgtcttccc agcaggcgac
     2221 ggcatcggtg tattggcgtt tgtcgtacca gagattgcca agataatagg gggcacgggc
     2281 atcggcgggg ttgagttttt gtgcgtcccg gaggatcagg acttcttcga tcttgttcgg
     2341 gaaacagcag gcgggatctg tttttgccgc tgtcctgtaa tgggcgatgg cggcttcttt
     2401 ttgaccggac agtgaggcga accatccgcg atagtagccg aggagcggcg agttcgcagg
     2461 gcaacgttgc aagacggtgt ccgcgtcttc atacagacct gcctgggcgt agtccagcgc
     2521 cagttcgagg taattattca atgacccgcg catccggatg tcgaactggt ccgagccttg
     2581 ctcgaacaag cagccgaagt tgaaggggtc cagggtgagg gagtccatga tgtacggctc
     2641 cgccgccttt tgcgttttcc gcaggagcac cgccttcagg tgacgagcca cggggctgtg
     2701 gtagttgcgg accagggatc tctcaacgag ttcgagggct tccttatatc ggtgttgggc
     2761 cgtcgcgatg cgcgccagcg acaggaatcc gctgtgttgc caggcgtcgt tccaggtggc
     2821 tttgtaaaac gcatcgaagg cttcttccag ccggccctgg agcttgagcg accagccgag
     2881 attataataa ggctcgccgt cataggggtt tggattgcgc cgggtgaggg aggcgatggc
     2941 cctacggaaa tggggctccg ccgcttcgaa ttgtccgcgg cgcaacagca gcaagcccat
     3001 cgcgttgttg cagcggatat cttccgggct tctgcgaagc ccttcggcat aataatcgat
     3061 ggggttatag gtagcatgcc ggtattgttc gagatgtagt ccgttcaggt aaaggtcttc
     3121 cacctgtgca atatcggcgg gagcgggtgc ggccgtggca ggcgcgggga tcgttctctt
     3181 ctctgtgccg ggatcgctgt cctgccaggc taccaatacc tggttctgtg catcggtaac
     3241 gaccagcgcc cagtcgccct ggcttccttt ttcggcagga aggtgggttt cttcgaatac
     3301 ctgctgtgga gagatgcggc gggtccattc ttttacgacc tggcccttgt tcagcagcaa
     3361 gacccgggcg ccggggtagt ctgacgtcgt atagaccttg acgtgcaggg tgtcgccttc
     3421 ccactgcaga ttaagcatcg cttcttttgt ggcgttcttt acctgtccca ccaatgtgta
     3481 gggcatgaag tattgttcga agcttttttc ttcgttgggc tgcaaccagg aaaaatccgg
     3541 ctggttgtcg gtaaagaccc cggtcatcag ctctatatag ggcccatcct cgtcggtgag
     3601 gtttctg
//
LOCUS       DATGFG010000744         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_281896, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000744 DATGFG010000000
VERSION     DATGFG010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281896"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..521)
                     /locus_tag="VKQ52_13915"
     CDS             complement(90..521)
                     /locus_tag="VKQ52_13915"
                     /inference="COORDINATES: protein motif:HMM:NF018009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HLZ88345.1"
                     /translation="MRRFLQEMGGDFTFVGNQYRLQSGEENYYIDILLYHRRLKSLIA
                     IELKAGKFKPAYTGQMNFYLAVLNDKVKTEEENPSIGIIVCKEKDRTTVEYALKEANN
                     PIGVASYKVTPSLPRDMKKYLPSPAEIVESLAGIIDVVKEK"
     gene            complement(403..1122)
                     /locus_tag="VKQ52_13920"
     CDS             complement(403..1122)
                     /locus_tag="VKQ52_13920"
                     /inference="COORDINATES: protein motif:HMM:NF036995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HLZ88346.1"
                     /translation="MAKLSLNKDYKQLLTEIKEKVYETQYRAMKQVNKALITLYWEIG
                     KGIAERQKRYKWGKSVVERLAEDLQKEFPGMEGLSSRNVWRMRMFYLEYEKLPPLVAE
                     IGWSHNMIIIEKCKDELRRKFYTAMTKKYGWTKNVLIHHIEGQSYEKYLLGQTNFDKA
                     VPEKYKHQAKLAIKDEYNFEFLELTDAHAEKRAGGCDYKAHAAVLTGNGWRLYVRRQS
                     VPVAVRGRELLYRHLAVSPAA"
     gene            complement(1343..1921)
                     /locus_tag="VKQ52_13925"
     CDS             complement(1343..1921)
                     /locus_tag="VKQ52_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ88347.1"
                     /translation="MYDKIAAYIRQRVSVPDEALEKAFAYGRVAEYRKGDLILRVGEY
                     CPFIGFINSGLIMVTLDDAEGREVICNFFFENEFFTYIESINGNIPSHKNFVALEDCT
                     VLLLNKSEMPAIFSIHPAFASLYNQTILEDLQKVIRYVQEQQTQTVEERYLSLMDRQE
                     NLFNRIPLKYIAGYLGVAPPSLSRLRKRLAGR"
     gene            complement(1947..>3347)
                     /locus_tag="VKQ52_13930"
     CDS             complement(1947..>3347)
                     /locus_tag="VKQ52_13930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLZ88348.1"
                     /translation="LLDEMGWGGCLADDMGLGKTLQTLSFLRYVVERYPEETHLVVCP
                     TSLLYNWEGELTKFVPGLDYYLHYGADRELDEERFRRAHVIITSYGMVRNDIGHFSRY
                     RFGYVILDESQAIKNPASQARKAIQQLQARNRLALSGTPVQNNTFDLYAQMDFLNPGM
                     LGSADFFRAEFAGPIDRNGDREAALRLRKLIYPFVLRRTKEQVARDLPDKTEMILWCQ
                     MGEEQRKIYDSHREHYRESLLERIAEEGMGKSSIYILEGLTKLRQICDSPALLRQSDG
                     RPNQRGADGPAKGGNEGFPNVSVKLEELVREVTENTGDHKCLVFSQFTSMLGLIRTAL
                     EASGIAYLYLDGSVAAEDRRESVRRFQEEDGARVFLISLKAGGVGLTLTAADYVYLVD
                     PWWNPAAEQQAIDRSHRIGQAKKVFAYRMICKDTVEEKILQLQEKKRSLAADLVADEA
                     GFVKQLTSEDVAYLFG"
BASE COUNT          725 a         1093 c          690 g          839 t
ORIGIN      
        1 ccgtaataaa ataccgcacc cacctatttt cctgtcttgt cgtgtaggct tgcaagagcc
       61 cctatttctt gcaccgtcgg tgcaagtctt tatttttcct tcacaacgtc tattatcccc
      121 gcaaggcttt cgacgatctc cgcgggggaa ggaaggtatt ttttcatgtc cctgggcaag
      181 gatggcgtta ctttatagga agctacccca atcgggttgt tggcttcctt tagcgcatat
      241 tctaccgttg tcctgtcttt ctctttgcaa acgatgatac cgatggaagg gttttcttct
      301 tctgtcttta ccttatcatt caacaccgct aggtaaaaat tcatctgacc ggtataggcc
      361 ggcttgaact ttccggcctt caactcaata gcaatcagcg atttaagccg ccggtgatac
      421 agcaagatgt cgatataata attctcttcc cctgactgca accggtactg attgccgacg
      481 aacgtaaagt cgccacccat ttcctgtaag aaccgccgca tgtgctttat aatcgcagcc
      541 tccagctctt ttttccgcat gtgcatcggt cagttctaga aattcgaagt tatattcatc
      601 ctttatcgcc agcttagcct ggtgcttgta tttttccgga acagccttat caaagttagt
      661 ttgacccagc aggtactttt catagctctg cccctcaata tgatggatta atacgttctt
      721 cgtccatcca tattttttcg tcatggccgt ataaaatttc cgcctcagct cgtctttgca
      781 tttctcaata atgatcatat tatgactcca gcctatttct gccaccaatg gtggcagttt
      841 ttcatattcc aggtaaaaca tgcgcatccg ccagacattc cggctgctta atccctccat
      901 gccggggaac tccttttgca agtcttccgc cagccgctcg acaacagatt tgccccactt
      961 gtaccgcttc tgccgctctg caatgccttt tccgatctcc caatataagg taattaacgc
     1021 tttgttcacc tgcttcatgg cccggtactg cgtctcatag accttctctt tgatctccgt
     1081 caacaattgc ttgtagtcct tgtttagcga taactttgcc attcctgtaa aattatttga
     1141 tttcacttct catagcggat ggaccttttt cacatccctt tacaggtcat cgggtacctg
     1201 ccgcttgtag tcttccgtcg agctgacatg ccagtggccg gctatgtatc gaagttcttc
     1261 gccgaagcca tattttgcgc aggtgcacta gcatcacgcc cgttacattg tgaaactgtt
     1321 ctcccaaggg ccccgccccc cctcacctcc ccgccagcct cttccgcagc cggctaagac
     1381 tgggcggcgc cacccccagg taacccgcga tatacttcaa cggtatccgg ttgaacaggt
     1441 tctcctgccg gtccatcagg ctcagataac gctcctccac cgtctgcgtc tgctgctcct
     1501 ggacatacct gatcaccttt tggagatcct ccagtatcgt ctggttatat agggaagcaa
     1561 aagccggatg aatcgaaaaa atagccggca tctcgctctt gttcagcaac aacaccgtac
     1621 aatcctccag cgcaacgaaa ttcttatggg aaggtatatt gccgttgata ctctcgatat
     1681 aggtaaagaa ctcgttctca aaaaagaaat tacaaatgac ctcgcgcccc tccgcatcat
     1741 ccagcgtcac catgatcagc ccggaattga taaacccgat gaacggacaa tactctccca
     1801 cccgcaggat cagatccccc ttccggtact ccgccacccg gccataggca aaggccttct
     1861 ccaatgcctc atccggcacc gacaccctct gccggatata ggccgcgatc ttatcgtaca
     1921 taaactgatt attgatgcaa aatagcctaa ccgaacaaat acgccacgtc ctcgctcgtt
     1981 aactgcttca caaaacccgc ttcgtcagcc accaaatcgg ccgccaggct ccgcttcttc
     2041 tcctgcagct gcaggatctt ctcctccacc gtatccttac agatcatccg gtaggcaaac
     2101 accttcttcg cctggccgat gcgatgactg cggtcgatgg cctgctgctc ggcagccggg
     2161 ttccaccagg gatcgacaag atacacataa tccgccgccg tcagcgtcag ccccaccccc
     2221 cccgccttca ggctgatcag gaacacccgc gccccgtcct cctcctggaa gcgacgcacc
     2281 gactcccgtc gatcctccgc cgccacactc ccatccaggt acaggtaggc aatcccggaa
     2341 gcctcgagcg ccgttcgtat cagccccagc atcgacgtga actgcgagaa caccaggcac
     2401 ttgtggtcac ccgtattttc ggtaacctcc cgaaccagct cctccagctt gacactcaca
     2461 ttcggaaagc cctcatttcc gccttttgcc ggaccatcgg cgccccgctg attcgggcgc
     2521 ccgtccgact gccgcagcaa cgccggactg tcgcaaatct gccgcagctt cgtcaacccc
     2581 tctaaaatat aaatactgct cttccccatc ccctcctcgg cgatccgctc cagcaggctc
     2641 tcccggtaat gctcccggtg actgtcgtag atcttccgct gctcctctcc catctggcac
     2701 cataagatca tctccgtctt gtcgggaagg tcccgcgcca cctgctcctt cgtccgtcgc
     2761 aggacgaacg ggtagatcaa tttccgcaac cgcaacgccg cctccctgtc cccgttccgg
     2821 tcgatcggcc ccgcgaattc cgcccggaag aaatcggcgc tccccaacat ccccgggttt
     2881 aaaaaatcca tctgggcata aagatcgaag gtattgttct gcaccggcgt cccgctgagc
     2941 gcaagccggt tacgcgcctg caactgttgt atcgcctttc gcgcctgcga agccgggttt
     3001 ttgatagcct ggctctcgtc gaggatcaca tacccgaacc ggtatcggga aaaatgcccg
     3061 atatcattcc gcaccatccc ataactcgta atgatcacat gcgcgcgccg gaaccgctcc
     3121 tcatccagct cccggtccgc cccataatgg aggtagtagt caagcccggg aacaaacttt
     3181 gtcaactccc cttcccaatt atataacaac gatgtcggac acaccacgag gtgcgtttcc
     3241 tccggatatc gctccacgac atatcgcagg aagctcaggg tctgcagggt cttccccaat
     3301 cccatatcat ccgcaagaca gccaccccag cccatctcat ccagcag
//
LOCUS       DATGFG010000745         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_569779, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000745 DATGFG010000000
VERSION     DATGFG010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569779"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2942
                     /locus_tag="VKQ52_13935"
     CDS             <1..2942
                     /locus_tag="VKQ52_13935"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ88349.1"
                     /translation="HNLTFKEEAAEYGLADIGYNTQAVFFDYDRDGDLDCYLTRYLLN
                     GPNANTVFPKDLSGNSPANDKLYRNDGDARGLGHPIFTDVTAQANIRDCGYGLGVVAS
                     DLNNDGWPDLYVSDDFVSDDLLWLNNHNGTFTDRLHAATSHTSYSSMGVDAADLNNDG
                     LPDIVTLDMLPEYNRRKKMSFSPMNYERYQLERSMGYQPQFTRNMLQLNNGAWQRGDT
                     LIPYFSEIGQLAGISATDWSWSVLLADFDNDGWKDIHITNGIGRDFINSDFLDFSNTI
                     FSTARTKEEQHRLIKEKLASLDHVNLANYLYLNRQNDRFEDASASAGIDLPSMSAGAA
                     YVDLDNDGDLDLVVNNINKEPFVFINNTIQKGRTPTRHFLRIRLKGDPPNTAGFGAKL
                     WLAAGGNRQMQEQSPVKGYLSSVDQTLLFGLGQNTSIDSLTIDWPDQRRQTLYHLPPD
                     SAITVFQKNASLPAPQKPRNDIPLFTEASAATNILFRHRENSYNDFASQLLLPQKYSQ
                     LGPFIATGDINKDGLTDFFVGGAFNNSGKFFIQQRDGRFTSQTLSDSTKWQEDMDCVL
                     FDADGDGDLDLLVTCGDRLYPENSPYYIPRLYLNDGKGHFTLAKDAIPADVKTSAGCI
                     SIGDLDGDGQPDIFIGGRVSRQYPLPPRSFILRNDKGRFTDVTATVCPAISRAGMITS
                     AKWMDFDNDRHTDLVIAGEWMPIRFFKNNHGHLDEVSGSTGLTQTNGMWRTLAVADVD
                     GDGDLDIIAGNLGLNNDYQVNEQQPMELFATDVDHNGSIDPVLCYYIKDEDGVRRSYP
                     AVSRKELAQQIPLIKKRFLLNRDYAQATFSDICRPARGDSLLHFICDETRSCWFENLG
                     HGKFAKHVLPVEAQFAPVNAIVCADLDHDGVLDLVLAGNEYQAEVMHGRYDASYGCFL
                     KGIAVRQPHSPAGNMEERFTCLPGRAIGLRLTGDIKSMALIPVASGSGLLLAAANDDS
                     LRVLKIK"
     gene            2947..>4448
                     /locus_tag="VKQ52_13940"
     CDS             2947..>4448
                     /locus_tag="VKQ52_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HLZ88350.1"
                     /translation="MRTHKNTPVRSIFGAVPRAALRLAARPRAAGTRATLSRAAVTLA
                     IVALTSITLAAVLLFPSLQKRSRLRELRARYQAGMTPINDKNNVFYPEARMHYYDSLS
                     QQPQNTPRQRMAAEYGKAEAMLQLGREKAAIQLLMHVVDILKQNPGQAPGEDPRKLLA
                     LAWLRQGERNNCVSFHSSGSCIFPIEGSGIYSDPSATQKGIDLYQQILTDDPDDLASR
                     WLLNLAYMTIGKYPSAVPAAWLIPGLDKDSGTVKVKAFRDIAGGLGLTDSRNEAGGAI
                     VEDFDNDGYLDLVTSDWDPENGMHYFRNNADGGFTDLSGPSGLSRIKGAFNLLQVDYN
                     NDGFTDIFILRGGWRGEYGKMPKTLLRNNGDGTFTDVTVESGLLSLHPTQTAVWADFN
                     NDGWPDVFIGQETSSVDDPHPCELWINNQDGTFSEVARQAGCDLRGFVKGVASADYDK
                     DHWPDIFLSLRDGRKILLRNKGVHSNIPQFGNVTHRAGLDRDTTFTFPTW"
BASE COUNT         1022 a         1409 c         1129 g          888 t
ORIGIN      
        1 agcataacct tacctttaaa gaagaagcgg cagaatacgg cctggcggat attggctaca
       61 acacccaggc cgtatttttc gattacgacc gggacggcga cctcgattgc tatctcaccc
      121 gttacctgct caacggcccc aatgccaata ccgttttccc aaaggacctc agcggcaatt
      181 cccccgccaa cgacaaattg tatcgcaacg acggcgacgc ccggggtctc gggcacccca
      241 tttttactga cgtgaccgca caggcgaata tcagggattg cggctacggt ctgggcgtgg
      301 tagccagcga cctcaataat gacggctggc cggatctcta tgtctccgac gatttcgtct
      361 ccgacgacct tttatggctt aataaccaca acggaacctt taccgaccgc ctgcacgcgg
      421 ccaccagcca taccagctac tccagcatgg gcgtcgacgc cgcagacctg aacaacgatg
      481 gacttcccga catcgtcacc ctcgatatgc tcccggaata caaccggcga aaaaaaatga
      541 gcttctcccc catgaactat gagcgctacc agctggagcg ctccatgggc tatcagccac
      601 agttcacgcg caatatgctc cagctgaaca acggcgcctg gcaacgcgga gataccctca
      661 ttccgtattt cagcgagatc ggtcagctgg cgggcatctc cgccacggac tggagctgga
      721 gcgtgctgct ggccgacttc gacaacgatg gctggaaaga catccatatc accaatggca
      781 tcggccggga ttttatcaat tcggacttcc tcgatttcag caataccatc ttctccacgg
      841 ccaggaccaa agaggaacag caccggctga tcaaagaaaa attggcctcg ctggaccatg
      901 tcaacctggc caactacctt tatcttaacc ggcaaaatga tcgctttgaa gacgcttccg
      961 catcggccgg catcgacctg ccctccatgt ccgccggcgc cgcctacgtg gacctcgata
     1021 acgacggtga cctcgatctc gtagtgaata atatcaataa ggaacccttt gtctttatca
     1081 acaatacaat tcaaaagggt cgaacgccca cccgccattt tttacgcatc cggctaaaag
     1141 gcgatccgcc caatacggcc ggcttcggcg caaaactatg gttagcggcc ggcggcaacc
     1201 gccaaatgca ggaacaaagc cccgtgaagg ggtatctttc atccgtggat caaacccttt
     1261 tgttcggact tggacagaat acctccatcg attccctgac catcgactgg ccggatcaac
     1321 gacggcagac cctgtatcac ctccccccgg atagcgccat caccgttttt caaaagaatg
     1381 cttcccttcc cgccccccaa aagccgcgca atgacattcc ccttttcaca gaggcttcgg
     1441 cggcaaccaa tatcctcttc cggcacaggg aaaatagcta taacgatttc gcttcacaac
     1501 tcctgctgcc gcagaaatac tcccagctcg gtccttttat cgccaccggc gacatcaaca
     1561 aagacggcct tacggatttt tttgtcggcg gtgcctttaa taattccgga aaattcttta
     1621 tccagcaacg cgatggccgc tttacttccc aaactttgtc ggacagcacc aaatggcagg
     1681 aagatatgga ttgcgttctg ttcgatgcgg atggcgatgg cgacctcgac ctgctggtca
     1741 cctgcggaga ccggttatac cccgaaaact ccccctatta tatcccacgg ctctatctca
     1801 acgacgggaa aggccatttt accctggcga aagatgcgat ccccgccgat gtgaagacat
     1861 ctgccggttg catcagcatc ggcgacctcg acggcgacgg ccagccggat attttcatcg
     1921 gcggcagggt ttcccggcaa tatcccctcc ctccccggag ctttatcctc cggaacgata
     1981 aaggccgttt cacagacgtc accgcaaccg tctgcccggc gatcagccgc gcgggaatga
     2041 tcacctcggc caaatggatg gatttcgaca acgaccggca caccgacctg gtcatcgcgg
     2101 gggaatggat gcccatccgt ttctttaaga acaaccacgg acatttggat gaagtctccg
     2161 gttcaacggg cctcacacag accaacggca tgtggcgaac cctggccgtc gcggacgtgg
     2221 acggagatgg cgacctcgat atcatcgccg gtaaccttgg cttgaacaac gactaccagg
     2281 tcaacgaaca acaacccatg gagttgtttg ccaccgacgt ggatcacaac ggcagcatcg
     2341 accccgtgct ctgctattat atcaaggacg aggatggtgt cagacgttcc tatcccgccg
     2401 tcagccggaa agaactggcg caacagatac ccctgatcaa aaaacggttc ctgctcaacc
     2461 gcgactatgc tcaggccacc ttcagcgata tctgccggcc cgcccgggga gacagcctgt
     2521 tgcatttcat ttgcgacgag acacgcagct gctggttcga gaacctgggc cacggtaagt
     2581 tcgcaaagca tgtccttccg gtggaagcac aatttgcccc cgtaaatgct atcgtctgcg
     2641 ccgacctcga tcatgacgga gtgttggatc tcgtcctcgc cggaaatgaa taccaggcag
     2701 aagtgatgca cgggcgatac gacgcctctt acggctgttt tttgaaaggg atcgcagtgc
     2761 ggcagccgca ttcaccagcc ggaaacatgg aggaaaggtt tacctgtctg ccgggaagag
     2821 ccatcgggtt gcgcttgacg ggggatataa aaagcatggc cctgattccc gtcgcctccg
     2881 ggtccggtct cttgctggcc gcagccaacg acgattcact ccgggtactg aaaataaaat
     2941 gatctaatgc gtacgcataa aaatacaccg gtcaggagca ttttcggggc tgtcccccgc
     3001 gccgctctcc gcctcgccgc ccgcccccgc gccgccggca cccgcgccac tctctcccgc
     3061 gccgccgtca ccctcgccat tgtcgccctc acctccatca cccttgccgc cgtcctgctc
     3121 ttcccctccc tccaaaaacg ctctcggcta agggagctgc gggcacgata ccaggcaggc
     3181 atgacaccca tcaacgataa gaacaatgtc ttttaccccg aggcccgcat gcattactac
     3241 gactcgctct cgcaacagcc gcaaaacaca ccgcgccagc gcatggccgc ggaatatggc
     3301 aaggcggagg ccatgctgca gctcggccgg gaaaaggccg cgatccaact gctcatgcat
     3361 gtggtcgaca tcctgaagca gaatccgggt caggcgcccg gcgaagatcc ccgcaagctc
     3421 ctggccttgg cctggctccg gcaaggggag aggaacaact gcgtttcctt ccattcctcc
     3481 ggctcctgta tcttcccgat agaaggtagt ggcatctact ccgacccctc cgccacacaa
     3541 aaaggcatcg atctctacca gcagatcctc acggatgatc cggacgatct cgcatcccgc
     3601 tggctgttga acctcgctta catgaccatc ggaaaatacc cctccgccgt acccgccgcc
     3661 tggctgatac ccggactgga caaggacagc ggcacagtca aagtcaaagc cttccgggat
     3721 atcgcgggcg gtcttggact gactgacagc aggaatgagg cgggcggcgc catcgtggag
     3781 gatttcgata acgacggtta tctcgatctg gtcacctcgg actgggaccc cgaaaatggg
     3841 atgcattatt ttcggaacaa cgccgacggc ggttttaccg atctctccgg cccgtcggga
     3901 ttgagcagga tcaaaggcgc gttcaatctc ctgcaggtgg actacaacaa cgacggattc
     3961 accgatatct tcatcctgcg cggtggctgg cggggcgaat atggaaaaat gccgaagacc
     4021 ctgctgcgga acaacggcga cggcacattt acggatgtga cagtcgagag cggcctcctt
     4081 tccctgcatc ctacgcaaac cgccgtttgg gccgatttca acaatgatgg ctggcccgac
     4141 gtcttcatcg gccaggagac ctcgtccgtc gatgacccgc atccctgcga attgtggatc
     4201 aacaaccagg acggaacatt cagcgaagtg gcccggcagg ccggatgcga tctccgggga
     4261 ttcgtcaagg gggtggcttc cgccgactat gacaaggatc actggccgga tattttcctc
     4321 tccctgcggg atggccgcaa gatactcttg cggaataaag gcgtgcattc gaacatcccg
     4381 caattcggga acgttaccca tcgggcagga ctcgacaggg atactacctt taccttcccc
     4441 acctggtt
//
LOCUS       DATGFG010000746         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_466941, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000746 DATGFG010000000
VERSION     DATGFG010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466941"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VKQ52_13945"
     CDS             <1..364
                     /locus_tag="VKQ52_13945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ88351.1"
                     /translation="PTVPESPTIYYRVRETDRHGSSLYSNIVAVKQSVYYLDLAIAPN
                     PSPGSATLRFRNDRSTVVTIRLLDITGQPAWTRQYPATRGLNTLQLDHLPQLPNGMYI
                     VQMYNGENQQNVKFCIRR"
     gene            465..>2055
                     /locus_tag="VKQ52_13950"
     CDS             465..>2055
                     /locus_tag="VKQ52_13950"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ88352.1"
                     /translation="MASAGQTNPPGFSVTNYTSDNALPQNSINAMAFDRNGFLWLATE
                     MGLVRFDGRNFREYNSANSPLLLSNRCAGISLERSSGRIILEPEFSNHHILTVTDNYQ
                     LEVDSALSNPWQMNLRDNNQFSFAHLYKKWAAGNTSAFGGLFNGLENNGDFLTVNNSQ
                     AYVTKDQRYYFLDEHTAGVRPLSEIAGHAMKVQFIIGDVYILIDKQNHLYAYKDGNRQ
                     PVAGSTRLLDLLRQIDVKGAYLMQAKLRALRDTCHTFFAYKGNILLLSLHNGLVDFDT
                     LAANTSITSINCLIYDQRNKILYVGTATSGLYILKKQEFQRFGFTSDNYAINSLYAQV
                     ELSDGRLLTSSGILDPRNKINTPTPGIYDRQAIFRSSDDSIWYSSYGYVRKSDTGLHH
                     SSTVQAIGLGSWLASFVETPGGDVLFCSRGFLYRWHRGKTTLLLDRSAWPEDTEIYVL
                     ELINPNELWIGTSAGLFSYDLSHATLGFRPELGKATVRAIHKARDGGIWIGTYGQGFY
                     KYTGGRFIRMPADPGNNLATVH"
BASE COUNT          506 a          654 c          486 g          409 t
ORIGIN      
        1 gccaacagtc ccggaatccc ccacgatcta ctatcgcgtc agggaaaccg accgccatgg
       61 cagctccctg tacagtaata tcgtcgccgt aaaacaatcc gtatactacc ttgacctggc
      121 aatagcgccg aatccatcac caggatcagc cacccttcgc ttcaggaacg atcgcagtac
      181 ggtcgtcacc atccgcctgc tcgacatcac cggccaaccc gcgtggaccc ggcaataccc
      241 ggccacccgg gggctgaata ccctgcagct cgatcatttg ccgcaactac ccaacggcat
      301 gtatatagta cagatgtata acggtgaaaa ccagcagaac gtaaaattct gtatccggcg
      361 ctaaaaaatc taaattttat tgttgaactt agagcagcca gcaaaaaact atgacccgtc
      421 gcgtaagacc ccttctgcta attagtatac agctgctcct atccttggca tcggccggac
      481 aaacaaatcc gccggggttt agcgtcacca attacaccag cgacaacgcc cttccacaga
      541 acagcatcaa cgccatggcc tttgatcgca acggctttct ctggctggcc accgaaatgg
      601 gcctggtccg cttcgacggc aggaatttcc gggaatacaa ttcggcaaac tctcccctcc
      661 tgctgtccaa cagatgcgcc gggatcagcc tggaacggtc gtcggggcgg atcatcctgg
      721 aaccggagtt cagcaaccac cacatcctca cggtcacgga caattaccag ttggaagtgg
      781 atagcgccct ttccaacccc tggcagatga acctccggga caacaaccaa ttctcttttg
      841 cccacctgta taagaaatgg gcggccggca ataccagcgc cttcggtggg ctattcaacg
      901 gactggagaa caatggggat ttcctcaccg tcaacaacag ccaggcctat gtaacaaagg
      961 atcaacgcta ctatttcctc gacgaacata cggccggggt ccgccccctc tccgagatcg
     1021 ccgggcacgc catgaaagtc cagttcatca tcggagacgt ctatattctt atagacaaac
     1081 aaaaccatct ctatgcttac aaagacggga acagacaacc cgtcgccggc agcacccgcc
     1141 tgttggacct gctgaggcag atcgacgtca aaggcgccta cctgatgcag gcgaagctca
     1201 gggcgctaag agacacctgc cataccttct tcgcatacaa aggcaatatc ctgttgctaa
     1261 gcctgcacaa cggcctggtg gacttcgata ccctggccgc caacacctcc atcaccagca
     1321 tcaattgcct gatctacgac cagcgaaaca agatcctgta cgtaggcacg gctaccagcg
     1381 gcctgtacat tctcaaaaaa caggaattcc agcgattcgg cttcacaagc gacaactatg
     1441 cgatcaatag cctctacgca caggtggaac tgtcggatgg ccgcctgctg acctcttccg
     1501 gcatcctcga tccccgaaac aaaataaata ctccgacacc cggcatctac gaccggcagg
     1561 ccattttccg gtcttcagac gattccatct ggtattcttc ctacggctat gtaagaaaat
     1621 ccgacaccgg tctgcatcat tcttccaccg ttcaggccat cggcctgggc tcctggcttg
     1681 cctccttcgt ggagaccccc ggcggtgatg tccttttctg cagcaggggt ttcctgtatc
     1741 gttggcatag gggaaaaacc accctgctgc tcgaccgttc ggcctggccg gaagacaccg
     1801 agatctatgt gctcgaactg ataaacccga atgagctatg gatcggcacc tcggcaggcc
     1861 tgttctccta cgacctgtcc cacgccaccc tcggcttccg gccggagctg gggaaagcca
     1921 cggtaagggc gatccacaag gcccgggatg gagggatctg gatcggcacc tacggacagg
     1981 gcttttataa atataccggc ggtcgtttca tccggatgcc ggcagacccc gggaacaacc
     2041 tggccaccgt ccact
//
LOCUS       DATGFG010000747         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1615576, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000747 DATGFG010000000
VERSION     DATGFG010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615576"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VKQ52_13955"
     CDS             <1..229
                     /locus_tag="VKQ52_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88353.1"
                     /translation="AWALLADDKKDKAYAVFQQQVSGKPLEATELYWMGRLMEGMGKR
                     YNAGEFYKAAGKTRYDLDPADAAYIRKALEE"
     gene            complement(218..889)
                     /locus_tag="VKQ52_13960"
     CDS             complement(218..889)
                     /locus_tag="VKQ52_13960"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HLZ88354.1"
                     /translation="MRDPFTYPKQALLALTACFFCQPLWAHVIVGELEKLSRASAALL
                     YLKLGYQHILPLGLDHILFVLSLFLLSPRLKPVLWQATAFTIAHSVTLGLAMYRVITP
                     PTRIVESLIAISIFYVALENIFSPRLKKTRIGIVFLFGLVHGLGFAGALGQLGLPPNA
                     YLLSLVMFNVGVELGQMTVIVAAFLLVGKWFGGKPWYRQRVVIPLSVIIAAIAAFLTL
                     QRLFF"
     gene            complement(989..1510)
                     /locus_tag="VKQ52_13965"
     CDS             complement(989..1510)
                     /locus_tag="VKQ52_13965"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HLZ88355.1"
                     /translation="MKTVKKHLFWLTALACFASCQKHSPVPPSAFYDQADQMGRPAIN
                     TVFIVAADKDKFNETIPSKMAAAFGSEMKSSLLALNPGYTTNLLGLSADQFIGVLSTD
                     VLNASTSGPTTFYDGTHVLTGRALGDDVIDVELTLLFGGPNGAANPGLTSDHVNGNDV
                     AFLPEFPYLAQPH"
     gene            complement(1573..2253)
                     /locus_tag="VKQ52_13970"
     CDS             complement(1573..2253)
                     /locus_tag="VKQ52_13970"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HLZ88356.1"
                     /translation="MKRKKLVLMVACAVLIVGGIVYAADHMDAPAVKGQSTDITDLYV
                     FEGKNTGNLVFVGNSQGLLAPAATGTASFKPNTLIQFKIDNDGDNVEDLVIQAIYKDG
                     KVNFFGLCRPSFTGTRTKIEERNFLGSVEVTPYGAHTPIVATGSYGIKFFAGPRDDPF
                     FFDLDQFHKITGGMATSFNNPGTDTFAGTNVLSIVVEVPKSLLHAQKGAISTWLTTST
                     VETNDGSK"
BASE COUNT          588 a          593 c          768 g          536 t
ORIGIN      
        1 tgcctgggcc cttctcgccg atgataagaa agacaaggcc tatgccgtct ttcagcaaca
       61 ggtgtcgggc aagccgctgg aagcgacgga gctttactgg atgggccgtc tgatggaagg
      121 gatggggaag aggtataacg caggagaatt ctacaaagcg gccggcaaga cccggtatga
      181 cctcgatccg gccgacgccg cctatatccg gaaagcatta gaagaatagc cgttgaagtg
      241 tcaagaaggc ggcaatcgcg gcgatgatga cggagagcgg gatgacgaca cgctgacgat
      301 accagggctt cccgccaaac cattttccca ccagcaggaa ggcagcgacg atgaccgtca
      361 tttgccccaa ttccaccccg acattgaaca tgacgagaga caggagatag gcgttgggcg
      421 gaagtcccaa ttggccaagc gccccggcga aacccaggcc gtgcaccaat ccgaataaaa
      481 agacgatgcc tatgcgggtt ttcttcagcc ggggagaaaa gatgttctcc agggcaacat
      541 agaaaatgga gatggcgatg aggctctcca cgatccgggt ggggggagtg atgacccggt
      601 acatggccag acccagggtc acggaatgag cgatcgtaaa agcggtagcc tgccagagaa
      661 cgggttttag ccgcgggctg agcaggaaaa ggctaagcac gaaaaggatg tggtcgaggc
      721 ccagcgggag gatgtgctgg taacccaatt tgagatagag cagggccgcg ctggcccggg
      781 acagcttttc cagttcgccg acgatgacat gcgcccacag cggttggcaa aagaagcagg
      841 ctgtcagcgc cagcaacgct tgtttgggat aggtaaaagg atcgcgcata ggcttgtttt
      901 ttgaagaagg gtgtgccctg ggtgtccggc cggcagcatg agtaaaaggg aggaaagatc
      961 ctccctttac tttttgaatg ataaagaatc aatgaggctg ggccaggtag ggaaattcag
     1021 gaagaaaggc gacgtcgttg ccgttgacat gatcgctcgt gagacccggg ttcgcggcgc
     1081 cgttcgggcc accgaagagc agggtcagtt cgacatcgat cacatcgtca ccgagggccc
     1141 tgccggtcag tacgtgggta ccgtcgtaaa aggtagtggg cccgctggtc gaggcgttga
     1201 gcacatcagt ggacaggacg ccgatgaact ggtcggcgct cagcccaagc aggttggtgg
     1261 tatagcccgg gttgagagca agcaggctgg atttcatctc cgagccaaag gccgcggcca
     1321 tcttgcttgg gatcgtctcg ttgaatttgt ctttgtcggc cgcgacgatg aagaccgtat
     1381 tgatggcggg tcttcccatc tggtcggcct gatcatagaa ggccgacggg gggacagggc
     1441 tgtgtttttg acaggaagca aaacaggcaa gcgccgtcag ccagaacagg tgtttcttta
     1501 ctgttttcat tttgaactgc atttaatttt gtttgtgtga acaggacagg tgttgtagcg
     1561 gtcaagacaa ccttacttcg aaccgtcgtt ggtttccacg gtgctggtgg tcagccaggt
     1621 actgatcgcc cctttttgag cgtgcaggag cgacttggga acctcgacga cgatgctcag
     1681 aacattggtt ccggcaaagg tgtcggtccc ggggttgttg aagctggtgg ccattccgcc
     1741 ggtgatcttg tgaaactggt cgaggtcgaa aaagaaagga tcgtcgcggg gaccggcaaa
     1801 gaatttgatc ccatagctgc cggtggccac gatcggcgtg tgtgcaccat agggtgtcac
     1861 ttccacggag ccgaggaaat ttcgctcttc gatctttgta cgtgtgccgg tgaaggacgg
     1921 ccggcagaga ccgaagaaat tgaccttgcc atccttgtag atcgcctgga taacaagatc
     1981 ttcgacatta tcgccatcgt tgtcgatctt gaactggata agggtgttgg gcttgaagga
     2041 agccgtgccg gttgcggccg gcgccagcag cccctgcgaa ttgccgacaa atacgagatt
     2101 accggtgttc ttcccttcga atacatacag atcggtgatg tccgtggact ggccttttac
     2161 agccggcgca tccatatgat ccgcggcgta gacgatcccg ccgacgataa ggacagcaca
     2221 tgccaccatg agcacgagtt tttttcgttt cattgtaatt taattaaggt tgaaaaatcg
     2281 agaaagcgga cgtcgcgact tacggaaaac agaagcaaag agagggtatg gcggaatagt
     2341 tttcgataat catatacggg gccgctgttg gtctggattg cttttggggc tcatttttta
     2401 aaagccgtcg tctccactgg ttagcggccc cgcaattatt ttgcgacata taccgatgtt
     2461 tggctatttt taatccactt aactg
//
LOCUS       DATGFG010000748         8511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_456745, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000748 DATGFG010000000
VERSION     DATGFG010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456745"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VKQ52_13975"
     CDS             complement(<1..515)
                     /locus_tag="VKQ52_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ88357.1"
                     /translation="MLTISQRGQQMPASPIRKLVPFAEAAKKKGIKVYHLNIGQPDIE
                     TPKMALDAVRNFDFKVLEYSHSAGNESYRRKLAAYYKRVGIDVDYQQIIVTTGGSEAI
                     LFGFMGCLDAGDEVIIPEPFYANYNGFAVAANVVVKPLTSYIGNGFALPPVAEFEKLI
                     TPRTRAIVICNP"
     gene            complement(591..1028)
                     /locus_tag="VKQ52_13980"
     CDS             complement(591..1028)
                     /locus_tag="VKQ52_13980"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HLZ88358.1"
                     /translation="MKQLFLFASAIVLSVGVFAQGAQQKKAEDVVKFKELSYDFGKIK
                     QGTPVVHDFVFTNISSSPVIIESAMPSCGCTTPVKPEGAIQKGKDDKIAAGFNAQAVG
                     PFNKSITIKVAGIDMPLQLHITGEVLTADAYAKYEAAKTKTGR"
     gene            complement(1034..1504)
                     /locus_tag="VKQ52_13985"
     CDS             complement(1034..1504)
                     /locus_tag="VKQ52_13985"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HLZ88359.1"
                     /translation="MKKLAIAISLFTTGILLAPGVSAQAVNTAGTALPRPADVLQVKE
                     NSHNFGKIPQGRPATTTFEIVNTGSTPLKLDNVQASCGCTTPVWSRDPIEAGATAKII
                     VGYNAYAEGPFTKTVTVVYNTNMTRLLTISGEVYKAPASSAPDNASIQLLKQIN"
     gene            1739..4129
                     /locus_tag="VKQ52_13990"
     CDS             1739..4129
                     /locus_tag="VKQ52_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLZ88360.1"
                     /translation="MENLAPIEVLDLEKLSFDKFREEVLKDYRSACESREASLLGRKE
                     VLTGKAKFGIFGDGKEVPQVAMAKFFRPGDCYAGYYRDQTFAFATGAATIEEFFSQLY
                     ADPDPSHDPHSAGRQMNSHFATPFVDSDGSTLDLVNRKNLTAGLAPTAAQLPKALGLA
                     SASKAFREVKALQPFTELSNHGNEVCFCTIGDASTSEGHFWETLNAAGVLQVPLAIFV
                     WDDGYGISVPRQYQTTKGSISEVIKGFQKEDGTNGIDIYKVKAWDYAGMCEVFETGLR
                     AVRQTHTPALFHVEEVTQPQGHSTSGSHERYKSPERLEWEREWDGIKKLKEWIIDNAL
                     ASEDELNDIETEAKESVRNSRNLAWEKYQSPIRIQVTRSAELIREMAHDSPDHAEPLL
                     KLATALASNREPGRKEVLQTLHEAIGVAGSAAARKALAYYKELKTENEALYNTHLYHE
                     GARKILQVPEVKAVYTDNAPVLNGYEVLNRYFDQLFTHNPKVLAFGEDLGKIGDVNQG
                     FAGLQAKHGADRIFDTGIRELTIMGQGIGMAFRGLRPIAEIQYIDYLLYGLQPLSDDL
                     ASLQYRTAGKQTAPLIIRTRGHRLEGIWHSGSPMGLILHSLRGLHVCVPRNMVQAAGM
                     YNTLLEGADPALMIECLNGYRLKEKLPSNLLDFRVPLGEPEVLREGEDITLVSYGATL
                     RIVQEAAGAVEAFGISCEVIDVQTLLPFDVHHNILESLKKTSRILIVDEDVPGGAAGF
                     IFNKVMEEQGGYKWLDTAPRTITAQAHRPAYSSDGDYFSKPNAEDIIDAIREMIAE"
     gene            complement(4180..4902)
                     /locus_tag="VKQ52_13995"
     CDS             complement(4180..4902)
                     /locus_tag="VKQ52_13995"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLZ88361.1"
                     /translation="MGRIFEVRKHTMFARWDRMAKQFTRIGKEIAIAVKTGGPDPTTN
                     PALRRCFQNAKSVNMPKDRVEAAIKRAQGKEMENYEEILYEGYGPHGVAILVETATDN
                     HVRTVANVKSHFTKGHGSLGNNGSVSFQFRKMGVFKLKPEGLNMDDLELELIDHGLEE
                     MGEGTGEHDEPVIVLRVNFPDFGNMQKALEEKGITPISSETEWIPASTVELGEEQAQE
                     VLKLVDRLEQDEDVQKVFHNLK"
     gene            complement(4970..6454)
                     /locus_tag="VKQ52_14000"
     CDS             complement(4970..6454)
                     /locus_tag="VKQ52_14000"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLZ88362.1"
                     /translation="MSLFRKKSLDSLMAQAADSEHGLKRTLGVWSLIALGIGAIIGAG
                     LFVRTAAAAGEHAGSAVTISFLVAALGCCFAGLCYAEFASMIPIAGSAYTYAYTTMGE
                     LVAWIIGWALVLEYALGAATVSISWSEYLNKLLGGSIPFEWCHSPFERILDSSGVMHH
                     GIINIPALFILLLLSLLLMKGTQESATVNGIIVFIKVSIVLVFIAIGWQFINPGNHHP
                     YVIPADAPPATMRDGTLYSYASFFRHGWGGILSGAGIVFFAFIGFDAVSTAAQEAKNP
                     TKDMPMGILGSLAICTILYVLFSWVLTGVAPYTDFVKSGKEASVAYAIETYMHGYHWL
                     SVLVTIAILAGFSSVILVMLLGQSRVFYTMATDGLLPKVFCALHPKFRTPYKSNMLLF
                     LFVGLFSGFVPESVAGNLTSIGTLFAFILVSIGVVILRKSDPKRPRPFRTPFVPIVPI
                     LGVIVCTAMIVGLDSATQLTALIWMVIGLIIYFGYSRNNSKLKA"
     gene            6677..7921
                     /locus_tag="VKQ52_14005"
     CDS             6677..7921
                     /locus_tag="VKQ52_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HLZ88363.1"
                     /translation="MPKISLTGWILIAMVTGVLLGLWVHYNGSPGTIEGFTTVLGVVT
                     DVFLRLIKMIIAPLVFSTLVVGIAKLGDIKAVGRIGGKTLLWFMCASLLSLLLGLVIM
                     NALSLGSELNLPVPPSGAPTGIDASTITFKGFITHIFPRSIFEALAGNEILQIVVFSV
                     FFGIATASIGEQGKIVIKALDAVAHIILRVTGYVMYFAPIAAMAAIASIMAIKGPELL
                     VTYGKFILSFYLTILGLWLILLLIASFFLGRRVIDLAMNVKDMILLAFSTASSEAAFP
                     RLIVELENFGCSPKIVSFVLPLGYSFNLDGSMLYMTFASLFIAQAYGVPLTLGQELSM
                     LLVLMLTSKGIAGVPRAALVVIAGTLSLYNIPQQGLLLLLGIDQILDMARSATNVLGN
                     SIAAALVSKWEGELEETAVNIK"
     gene            7926..>8511
                     /locus_tag="VKQ52_14010"
     CDS             7926..>8511
                     /locus_tag="VKQ52_14010"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HLZ88364.1"
                     /translation="MLQLLLITWKDLLNPDFYINNGGLWVLLFIVFAETGLMVGFFLP
                     GDSLLFVAGIYSEKLISSMIAGGTGSEILDLLLLWLLVSICGIIGNSLGYWFGNKSGP
                     FLFHRKDNLLFKKKYLFQAREFYDKHGGEAIVFARFLPIVRTFAPIIAGIVAMNRKKF
                     VLYNIIGSLAWVFAMLFAGHYLDKLFITKFGFDLK"
BASE COUNT         1947 a         2527 c         2145 g         1892 t
ORIGIN      
        1 ggattacaga taacgatcgc ccttgtccgc ggtgtgatca gcttctcgaa ctccgccacc
       61 ggcggcaggg caaacccatt cccgatatac gaggtcaacg gctttaccac cacattggca
      121 gccaccgcaa acccgttgta attggcgtaa aaaggctcgg ggatgatgac ctcatcgccg
      181 gcatccaggc aacccataaa gccaaacagg atcgcttccg acccccccgt agtaacaatt
      241 atttgctggt agtccacatc gatacccacg cgcttgtaat aggccgccag cttccgccgg
      301 tagctctcat tgcccgcact atgactgtat tccaggacct tgaaatcgaa attccgaacc
      361 gcatccaacg ccattttggg cgtctcgatg tcgggctgac cgatattcag atgatacacc
      421 tttattcctt tctttttggc ggcctcggcg aagggcacga gcttccggat cggcgatgcc
      481 ggcatttgtt gtccgcgctg actgatagtt aacatggcgg caaaattagg gaaaagtaaa
      541 aagaaagagg ccgtccatcg gacggcctct ttacctgtat aatgaatgaa ttatcgtcct
      601 gtcttggttt tggcagcctc gtacttggca taggcatccg cggtgagcac ttcacccgtg
      661 atgtgcagct gaagcggcat atcgataccg gcaaccttga tcgttatgga tttattgaaa
      721 ggacccactg cctgcgcgtt gaaaccggcc gcgatcttgt catccttgcc cttctggata
      781 gcgccttcgg gctttacggg tgtcgtacag ccacaggaag gcatcgcgga ctcgatgatc
      841 accggcgaac tgctgatatt cgtaaataca aaatcatgga ctacaggagt gccctgcttg
      901 atcttcccaa aatcatacga aagctctttg aacttcacta catcttctgc tttcttttgt
      961 tgtgcgccct gggcaaaaac acctacggat aagacaatcg cgctggcgaa taggaatagt
     1021 tgcttcattt tgatcagttt atttgtttta gtaattgtat cgatgcatta tccggcgccg
     1081 aactggccgg ggccttgtac acctccccgc tgatggtgag gagcctggtc atgttggtat
     1141 tgtaaaccac ggttacggtc ttggtaaaag gaccctccgc ataggcattg tagccgacaa
     1201 tgatctttgc cgtcgcaccc gcctcgatgg ggtcacggct ccagaccggc gtggtgcaac
     1261 cgcaggaagc ctggacattg tccagcttta acggcgtcga acccgtattc acgatctcga
     1321 atgtagtagt ggccggccgc ccctgcggga tcttgccaaa attatggctg ttctccttca
     1381 cctggagcac atccgccgga cggggtaggg cagtccctgc cgtattcacc gcctgggcgc
     1441 tgacgcccgg cgccaggaga attcctgtgg taaaaaggct gatcgcaatt gctaattttt
     1501 tcatagtttg gctgcgttaa ggttttgctt cgccgggcta aacaaaaaaa tggcccgtct
     1561 gcgcaaaacg gctaattgat tgaccttcgg tcggccggcc cgtggtcggc cccagtccta
     1621 aaattactta cttaacgcca actttaccaa taaattaccg gcaattttat ttttttaaca
     1681 atcaataaga tacaggtaat tttgcccaac catcgatcgc ttttcaaata tcattctaat
     1741 ggaaaatcta gctcccattg aagtcctcga tctcgaaaaa ctgtccttcg acaagttccg
     1801 ggaagaagta ttaaaagact accggtcggc ctgcgaaagc cgcgaggcca gcctcctggg
     1861 ccgcaaggag gtcctcacag gcaaggccaa attcggcatc tttggcgatg gcaaggaagt
     1921 accccaggtg gcgatggcca aattcttccg gcccggggat tgctatgccg gctactatcg
     1981 ggaccagacc tttgccttcg caacgggagc agccaccatc gaagaattct tttcacaatt
     2041 atatgccgac ccggatccca gccacgaccc gcacagcgcc ggccggcaga tgaactcgca
     2101 ttttgctacg ccattcgtag attccgacgg ctcgacactc gacctcgtca accgcaagaa
     2161 cctcaccgcc ggactggccc ccaccgccgc acagttgcca aaggccctcg gcctcgccag
     2221 cgcctcgaag gccttccgcg aagtaaaggc gctccaaccc tttaccgagt tgtccaacca
     2281 cggcaacgag gtctgctttt gtaccatcgg cgacgcctcc accagcgagg gacatttctg
     2341 ggaaaccctc aatgccgcgg gagtattgca ggttcccctg gccatcttcg tctgggacga
     2401 cggctatggc atctccgttc cccgccaata ccagacgaca aagggatcca tctccgaggt
     2461 aataaagggt tttcaaaagg aagacggcac caacggcatc gatatctata aggtaaaagc
     2521 ctgggactat gccggcatgt gcgaggtctt cgagaccggt ctccgcgccg tcagacagac
     2581 ccataccccg gcgctcttcc acgtcgagga agtgacccag ccccagggac attccacttc
     2641 gggcagccat gaacggtata aatcccccga acgcctcgaa tgggaacggg aatgggatgg
     2701 gataaagaag ctgaaggagt ggatcatcga caatgccctt gccagcgagg acgaactcaa
     2761 cgacatagaa accgaggcaa aggaatcggt tcgcaacagc cgtaacctgg cctgggaaaa
     2821 ataccagtct cccatccgca tccaggtcac ccgcagcgcc gaactcatcc gcgagatggc
     2881 ccatgactcc cccgatcacg ccgaaccctt gctcaaactg gctaccgccc tggcttccaa
     2941 ccgcgaaccg ggccgcaaag aggtgctcca gaccctccac gaagccatcg gggtggccgg
     3001 ttccgccgcc gcccgcaaag cccttgctta ttataaggaa ttaaagaccg aaaatgaggc
     3061 cttgtataat acccatcttt accatgaggg cgcccgcaag atcctccagg ttccggaggt
     3121 aaaggccgtt tacaccgaca acgcacccgt tcttaacggc tacgaggtcc tcaaccgcta
     3181 ttttgaccag ttgttcacgc ataatccaaa agtactggcc tttggggaag acctcggaaa
     3241 gatcggggat gtcaaccagg ggtttgccgg actacaggcc aaacatggcg ccgaccgcat
     3301 cttcgacacc ggcatccgtg aactcacgat catgggccag ggcatcggca tggccttccg
     3361 gggactgcgg cccatcgccg agatccaata catcgattac ttattatatg gcctgcagcc
     3421 actgagcgat gacctggcct ccctgcagta ccggacggcc ggcaaacaaa cggcccccct
     3481 catcatccgc acccgcggcc accggctcga aggcatctgg cacagcggtt cacccatggg
     3541 gttgatcctg cattccctcc gtggtctcca tgtgtgcgtg ccccgcaata tggtccaggc
     3601 cgccggcatg tacaacaccc tcctggaggg cgccgacccg gccctgatga tcgaatgcct
     3661 gaacggctac cgcctgaagg aaaagctccc gtccaatctc ctcgatttcc gtgtgccgct
     3721 gggcgagccc gaggtattgc gggaaggaga ggacattacc ctggtatcct atggggccac
     3781 cctgcggatc gtccaggagg ccgccggggc cgtggaggcc tttggcatta gttgcgaggt
     3841 catcgatgtc cagaccctcc tgcccttcga cgttcaccac aacatcctcg aatccctgaa
     3901 aaagaccagc cggatcctca tcgtcgacga ggacgttccg ggcggcgccg cgggattcat
     3961 ctttaataag gtgatggaag aacagggggg ctacaaatgg ctggacaccg caccccgcac
     4021 gatcaccgcc caggcccacc ggcccgccta ttccagcgac ggggactatt tcagcaaacc
     4081 caatgcggaa gatattatcg acgccatccg cgaaatgatc gccgaataaa caatagaata
     4141 gaacaataaa aaagggccgc atctcagcgg ccctttcttt tatttcagat tgtgaaacac
     4201 tttctgtaca tcttcatcct gctccagccg gtccaccagt ttcaacacct cctgcgcctg
     4261 ctcctccccc agttcgaccg tgctcgccgg gatccactcc gtctccgaac tgatgggagt
     4321 gatacctttt tcttccagcg ccttttgcat attgccaaag tccgggaaat tcacccgcag
     4381 aacgatcacc ggttcgtcat gttctcccgt tccttccccc atttcttcga gcccatgatc
     4441 gatcagctcc agttccaggt cgtccatgtt cagcccctcc ggctttagct tgaagacccc
     4501 cattttcctg aactgaaaac tcacgctgcc gttattgcca agcgacccat gccccttggt
     4561 aaaatgcgat ttgacattgg ccaccgtccg cacatggtta tccgtcgccg tctcgaccag
     4621 gatggcgacg ccatgcggac catatccttc atacaggatc tcctcgtagt tctccatctc
     4681 ctttccctgc gcccgtttga tcgcggcttc cacccggtcc ttgggcatat tcacgctctt
     4741 cgcattctga aagcagcggc gcagcgccgg attggtggta gggtcgggcc ccccggtctt
     4801 cacggcaatg gcgatttctt ttccgatacg ggtaaattgc ttggccatcc ggtcccaccg
     4861 ggcgaacatg gtatgctttc taacttcaaa aatgcgaccc ataaaaaact ataacttttt
     4921 gattatgtgc acttcatgag cctctttcga ggctccggcg gctgcaaagt tacgctttta
     4981 gcttgctatt attcctgctg tacccaaaat agattattaa tccgatcacc atccatatca
     5041 atgccgtcag ctgggtggcg gagtccagac cgacgatcat ggcggtacaa acgatcaccc
     5101 ccaggatggg cacgatcggc acaaaaggcg tcctgaacgg ccgggggcgc ttggggtcac
     5161 tcttacgcag gatcactacc ccgatgctga ccaggatgaa ggcgaacagg gtgccgatgc
     5221 tggtcaggtt gccggctacg ctttccggga caaaaccgga gaacaggcct acgaaaagga
     5281 acaacagcat attcgactta tagggggtac ggaatttggg atgtagcgca caaaacactt
     5341 tgggcagcag tccatcggtc gccatggtat agaatacccg gctttggcca agcaacatca
     5401 ccaggatcac ggacgagaac ccggcaagaa tcgcgatcgt taccagaacc gacagccagt
     5461 gataaccgtg catataggtc tcgatggcat aggcgactga agcctcttta cccgacttca
     5521 caaaatccgt ataaggcgcg acgccggtaa gcacccagga gaacaataca tacaggatcg
     5581 tacagatcgc cagcgaaccc aggatgccca tcggcatatc cttggtgggg ttcttggctt
     5641 cctgtgcggc ggtggatacg gcatcaaagc cgataaaggc aaagaagaca atacccgctc
     5701 ccgagaggat gcctccccag ccatgcctga aaaagctggc ataggagtaa agtgtgccat
     5761 cccgcatggt ggcgggcggc gcatcggcag ggataacata gggatgatgg ttgcccggat
     5821 tgatgaactg ccagccgatg gcgatgaata ccagtacgat ggaaaccttt ataaaaacga
     5881 tgatgccgtt taccgtagcg gactcctgcg tccctttcat caacagcagg gaaaggagca
     5941 acaggataaa cagggcggga atattgatga tcccgtgatg cattacaccg gaactgtcca
     6001 gaatcctttc gaaaggggaa tggcaccatt cgaagggaat gctccccccc agcagcttat
     6061 tgagatattc gctccaggaa atcgacactg tggcagcccc cagggcatat tccaatacca
     6121 gggcccagcc gatgatccag gctaccagtt cacccatggt tgtataggcg taagtgtatg
     6181 cacttcccgc aatagggatc atggaggcga attcggcgta acaaagtccg gcaaaacagc
     6241 aaccgagggc ggcaaccaga aaggagatgg ttacggccga accggcatgt tcccctgcgg
     6301 cggcggctgt ccgcacgaac aaccctgcgc cgataatggc gccgatcccc aacgcgatca
     6361 ggctccagac ccccagcgtc cgcttgagac cgtgctccga atccgccgcc tgcgccatga
     6421 gggaatccag ggatttcttc cgaaataaac tcataaatag cttgttttga taataatttg
     6481 gaaatatgat ttcatgtgat gactagggcc gaaaataaac aaatattcaa tactcaaacc
     6541 ccaattcttt aaccaaaatt agccctgggc aagagcgctg gccgggattt ttggcattaa
     6601 agttttgtca aatcaataat tttcgattgt ttgcatggtc aacggagggc caggaccgcc
     6661 catcaagcat tgacctatgc caaagattag tcttacagga tggattttga tcgccatggt
     6721 gacgggagtg ttgcttgggc tttgggtgca ttataatggt agcccgggga cgatcgaagg
     6781 ctttaccacc gtcctcggcg tcgttaccga tgttttcctg cggttgatca aaatgatcat
     6841 cgcgccactg gtcttctcca ccctggtggt cggtatcgca aaactggggg atatcaaggc
     6901 cgttggccgc attggcggca agacccttct ctggttcatg tgcgcctccc tgctttccct
     6961 cctgctgggc ctggtgatca tgaatgctct ctcgctcggc agcgaactca atctgccggt
     7021 tcccccctcg ggcgccccga caggcatcga cgccagcacg atcaccttca aaggatttat
     7081 cacccatatc tttccccgta gcatttttga ggccctggcc ggcaacgaga tcctccagat
     7141 cgtcgtcttc tcggtctttt ttggcatcgc taccgcctcc atcggcgaac aggggaagat
     7201 cgttataaaa gcactcgacg ccgtagcgca tatcatcctc cgcgttaccg gttatgtcat
     7261 gtattttgcc cccatcgcgg caatggcggc catcgcctcc atcatggcaa taaagggtcc
     7321 cgaactgctg gtcacctacg ggaaattcat cctcagcttt tacctcacca tcctcggcct
     7381 ctggctgatc ctgttgctga tcgcttcctt cttcctcggc cgccgggtca tcgatctggc
     7441 catgaatgta aaggacatga tcctgctggc ctttagcacg gcatcctcgg aagccgcgtt
     7501 tccccgcctg atcgtcgagc tggagaattt tggctgctca ccaaagatcg tcagcttcgt
     7561 cctgccgctg gggtattcct ttaacctcga cggcagtatg ctctatatga cctttgcctc
     7621 cctcttcatc gcccaggcct acggagtgcc gctgaccctc ggtcaggaac tgtccatgtt
     7681 gctggtgctg atgctgacga gcaagggtat cgccggggtg ccgcgggcag ccctggttgt
     7741 gatcgccgga acactttccc tttacaatat cccgcaacag gggctgctac tcctcctggg
     7801 catcgaccag atcctggata tggcccgcag cgccaccaat gtattgggga acagcatcgc
     7861 tgccgcgctc gtaagcaaat gggaggggga actcgaagaa accgctgtca atataaaata
     7921 actctatgct ccaactctta ctgatcacct ggaaagacct tttaaacccc gacttttata
     7981 tcaacaacgg cgggttatgg gtgctcttat tcatcgtatt cgcggagacc ggcctgatgg
     8041 tcggcttctt tttacccggc gacagcctgc tgttcgtcgc cggcatctat agcgagaaac
     8101 tcatcagcag catgatcgcc ggcggcaccg gcagcgagat cctcgacctc ctcctcctct
     8161 ggctcctcgt ttccatttgc ggcatcatcg gcaattccct cggctactgg ttcgggaaca
     8221 aaagcggccc cttcctcttt caccgcaagg acaacctgct gttcaaaaag aaataccttt
     8281 tccaggccag ggaattctat gataaacatg gcggagaggc catcgtcttc gcccgcttcc
     8341 tgccgatcgt ccgcaccttc gccccgatca tcgccggcat cgtggccatg aacaggaaga
     8401 agttcgtgct ctataatatc atcggcagcc tggcctgggt atttgccatg ctcttcgccg
     8461 gccactacct cgataagctc tttattacga agtttggctt cgatctgaaa a
//
LOCUS       DATGFG010000749         5536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1500058, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000749 DATGFG010000000
VERSION     DATGFG010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500058"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VKQ52_14015"
     CDS             <1..1251
                     /locus_tag="VKQ52_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLZ88365.1"
                     /translation="PDEALICSCESVTKAAICSAVTEKEVTTIDAMKKCTRAGTGCGG
                     CIPLVKDLITGTMKAGGLYVRNVVCEHFDYSRQELFDLVKLHDVRTYNDTLAHWGKGD
                     GCEVCKPVVASILASLWNETIAKQATIQDSNDRYLANIQKGGSYSVVPRIPGGEITPD
                     KLIVLGKVAKKYNLYTKITGGQRIDLFGAHVGDLPAIWEELIEAGFESGHAYGKALRT
                     VKSCVGSTWCRFGLHDSVSFAIEIEERYRGIRAPHKLKGGVSGCIRECAEAQSKDFGI
                     IATEKGWNLYVCGNGGSRPQHAQLLATDIDKAACVQYLDRFLMFYIRTADPLTRTATW
                     LNKMDGGMSYLKNVVINDSLGMGQQWEQEMQALVDSYKCEWKEVVENPELRKRFTHFV
                     NAPRKKDPGLQFDDMRGQIKAQEW"
     gene            1271..1657
                     /gene="nirD"
                     /locus_tag="VKQ52_14020"
     CDS             1271..1657
                     /gene="nirD"
                     /locus_tag="VKQ52_14020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HLZ88366.1"
                     /translation="MQTTNTTTATREAAAPVTKWLAACRVEDVPGDGGACVLLEGEQI
                     AIFYFSRRDQWYATENRCPHKQQMALSRGMTGSTGGDCEPKVACPFHKKTFSLRTGEC
                     LSGEDYRIRTYPVKVKNGKVYIGFPW"
     gene            1666..2616
                     /gene="moaCB"
                     /locus_tag="VKQ52_14025"
     CDS             1666..2616
                     /gene="moaCB"
                     /locus_tag="VKQ52_14025"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HLZ88367.1"
                     /translation="MRDITHKPVTLRTARAVGVIFCSAGTIPLIRNGQLPKGNLFDVA
                     RAAGFLGAKAVPQLLPHCHPVTIDGMDMEFEFLDPAAHGELLGAADAARTGIVITGEA
                     RSIGRTGIEMEILAGVTIAALELYDMLKPVDKQLEIGRVRLLEKRGGKSDRVSYFATP
                     PTCAVLVCSDSTAEGKREDKSGRIIQEMLEAVNARVVDYVVVPDEKSAIQQQVMQWVD
                     KDVQFIFTTGGTGLGPRDNTVTAIKELLERDADGIAEAMRAYGQQRTPMAMMSRGVAG
                     SVGHTLVVTLPGSSDGARESLEAILPAVFHARKMMKGGGH"
     gene            2633..3874
                     /locus_tag="VKQ52_14030"
     CDS             2633..3874
                     /locus_tag="VKQ52_14030"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HLZ88368.1"
                     /translation="MIDYRQAQEMVLQHAHSFGREAVPLEQAMGRVLAGTILADRDYP
                     PFDRASMDGYALRYNDLEKGIRRFAVIETLLAGYMPSRPLEAGECYKIMTGAAVPAGA
                     DIIIRRENTVEGKGVMTLPPVPAEALPAATLPAPADPGFPWRPFQNIARKGEDLVAGA
                     VVIDRACICEPPVIGLLATLGHTTVTVERLPAIGLLTTGDEVVAADAAVGAVQIRNSN
                     RWLLEAALKKTGAGPVSWDHAPDDPVLLWQRIDALLTNDILILCGGVSAGDADYVPGV
                     LEAAGVKKLFHKIAIRPGKPVWCGVTVDGKMVFALPGNPFSCLVNMVLLIRPYLQACY
                     GLRPAEPLGLALGTARPKRSPLDEFFPVYMHGSPARLTPVPVNGSGDIRLGMQANGLA
                     LHPAACGPLAEGDIIPCYSFV"
     gene            complement(3859..4545)
                     /locus_tag="VKQ52_14035"
     CDS             complement(3859..4545)
                     /locus_tag="VKQ52_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ88369.1"
                     /translation="MKKKVNTCDLQSCFLCKRCLPEWIPAIGTHRENLTYKKGEMIFQ
                     EGDEVKGIFFMYSGTVKVHKKWGTEKELIIRFASTGSLFGHRGLGRDHHYPISATALE
                     PVEACYFDLEFFQASLKVNQGFSLGLLQFFADELQASERKMRDIAHMQVKGRVAQALL
                     QLREDFGVTADGYIGLTLSRQDLSSLVGATYETVFRIINELAQEGLILLDGKNIAIAD
                     AIRLTDYTKE"
     gene            4849..>5536
                     /locus_tag="VKQ52_14040"
     CDS             4849..>5536
                     /locus_tag="VKQ52_14040"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HLZ88370.1"
                     /translation="MHKFITGMSILLLSYISLPVQGQLSLTGQLRVRTELRNGYGTLE
                     TIGSQPAFLTSQRARLNFNYRSSRVIFQASVQDVRVWGADASTINNADGSRLSIHEAW
                     GELVLANSHDSSFGHSPVEYFGIRVGRQELVYDDQRLLGNLDWLQQARRHDAIVFKLL
                     NKGWQTDLGVAFNQTTDAFNYNGTTYTPANVPAYVKDSRGNLAAVPTGYLPLVNAAGL
                     SARTGNPSFVV"
BASE COUNT         1146 a         1643 c         1623 g         1124 t
ORIGIN      
        1 cccgacgaag cgctgatctg tagctgtgag tcggtgacca aggcggccat ctgttccgcg
       61 gtgacggaaa aggaggtgac caccatcgac gcgatgaaga aatgcacccg cgccggcacg
      121 ggctgcggcg gctgtatccc actggtaaaa gacctgatca cgggcacgat gaaggccggc
      181 ggcctatatg tccgcaacgt cgtctgcgaa catttcgact attcccggca ggagctgttc
      241 gatctggtaa agctgcacga tgttcgcacc tataacgaca cgctggcgca ctggggcaag
      301 ggcgacggct gcgaggtctg caagccggtg gtcgccagca tcctggccag tctctggaac
      361 gaaacgatcg caaaacaggc gaccatccag gatagcaatg accgttatct cgccaatatc
      421 cagaagggcg gcagctattc ggtcgtgccg cggatcccgg gcggcgagat caccccggac
      481 aagctcatcg tcctgggaaa ggtggcgaag aaatacaatc tttatacaaa gatcaccggc
      541 ggacaacgta tcgatctgtt tggcgcgcat gtcggcgacc ttccggccat ctgggaggaa
      601 ctgatcgagg ccggcttcga gagtggtcat gcctatggca aggctctgcg aacggtaaaa
      661 agctgtgtcg gcagcacctg gtgccggttt gggttacatg acagcgtctc atttgccatc
      721 gagatcgagg agcggtatcg cggtatccgg gccccgcata aactgaaggg tggggtcagc
      781 ggctgtatcc gggaatgcgc ggaggcgcag agcaaggatt ttggcatcat cgccaccgag
      841 aaaggctgga acctctatgt gtgtggtaac ggcggttccc gtccccagca tgcgcagttg
      901 ctggccaccg atatcgacaa ggctgcctgt gtgcaatacc tggatcgctt cctgatgttc
      961 tatatccgta cggccgatcc gctcacccgt accgccacct ggctcaataa gatggacggc
     1021 ggcatgagct atctgaaaaa tgtcgtcatc aacgacagcc tggggatggg gcagcagtgg
     1081 gaacaggaga tgcaggccct cgtcgacagc tataaatgcg agtggaagga ggtggtggaa
     1141 aaccccgaat tacgtaaacg ctttacccat tttgtcaatg caccccggaa gaaagatccc
     1201 gggctgcaat tcgacgatat gcgcggccag ataaaggcgc aggagtggta atataaaaac
     1261 tgtttttccc atgcaaacaa ccaatactac aaccgcgacg cgcgaagccg cggcgcccgt
     1321 gaccaaatgg ctggctgcct gccgggtgga agacgttccc ggcgacggcg gcgcctgtgt
     1381 actgctcgaa ggcgaacaga tcgccatctt ctatttttcc cgccgcgacc agtggtatgc
     1441 caccgagaac cgctgcccgc ataagcagca gatggccctc tcccgtggga tgaccggcag
     1501 caccggcggc gactgcgagc caaaggtggc ctgtcccttt cacaaaaaga ccttctccct
     1561 gcggacgggt gaatgcctca gcggcgagga ctaccgcatc cgcacctacc cggtgaaggt
     1621 caaaaatggg aaagtgtata tcggctttcc gtggtaaatt gcgctatgcg cgacattact
     1681 cacaagccag ttacgctgcg tactgcccgg gcagtggggg tcattttctg tagtgccgga
     1741 accattcccc tgatccggaa cgggcagttg ccaaagggca acctcttcga cgtggcgcgg
     1801 gccgccggtt ttctcggcgc caaggccgtt ccgcagcttt taccccattg tcatcctgtt
     1861 accatcgacg gcatggacat ggaattcgaa ttccttgacc ctgcggccca tggcgagctg
     1921 ctgggcgccg ccgatgcggc gcgaaccggc atcgtcatca ccggcgaagc acggtccatc
     1981 ggtcgtaccg gcatcgagat ggagatcctc gccggggtca cgatcgcggc gctggagctg
     2041 tacgatatgc tgaagccggt ggacaagcag ctggagatcg gccgcgtccg gttgctggag
     2101 aagagaggag ggaaaagtga ccgcgtcagc tatttcgcca cgccgcccac ctgcgccgtg
     2161 ctggtctgct cggatagtac ggccgaaggg aaacgcgagg acaagagcgg caggatcatc
     2221 caggaaatgc tggaggccgt gaatgcccgg gtggtggatt atgtcgtcgt ccccgatgag
     2281 aaaagcgcca tccagcaaca ggtgatgcaa tgggtggata aggacgtcca atttatattc
     2341 accaccggcg gaacgggtct cggaccccgc gacaataccg tcaccgccat aaaggagctg
     2401 ctcgaacgcg atgccgatgg cattgccgag gcgatgcggg cttacggtca gcaacgtacg
     2461 ccgatggcca tgatgagcag gggcgtggcg ggctccgtcg gccatacgct tgtcgtcacc
     2521 ctgccgggca gcagcgacgg ggcgcgggaa tcgctggagg ccatccttcc cgccgtattc
     2581 cacgcgcgta agatgatgaa gggaggcgga cattaattaa tctattcagc caatgatcga
     2641 ttaccggcag gcacaggaaa tggtgctgca acacgcacac agctttggac gggaggccgt
     2701 tccactggaa caggccatgg ggcgggtgct ggccgggacc atcctggccg atcgcgacta
     2761 tccgcccttc gaccgggcct cgatggatgg ctatgcgcta cgctataacg acctggaaaa
     2821 ggggatccgc cggtttgccg tgatcgagac cctgctggcg ggctatatgc catcgcggcc
     2881 cctggaggcc ggcgaatgct ataagatcat gaccggcgcc gccgtgcccg ccggcgccga
     2941 tatcatcatc cgccgggaga atacggtgga gggaaagggc gtgatgacat tacctcctgt
     3001 tcctgccgag gccctgcctg cagctacgct gccggcgccc gcggacccgg gctttccctg
     3061 gcggcctttc cagaacatcg cgcggaaggg ggaagacctt gttgccggcg ccgtcgtcat
     3121 cgatcgtgcc tgtatttgcg agcccccggt gatcgggctg ctggcgaccc ttgggcatac
     3181 cacggtgacg gttgaacgcc tgcctgccat cgggctgctg accaccggcg atgaagtcgt
     3241 ggctgccgat gcggcggtcg gcgccgtgca gatccgcaac agcaaccgct ggctgctcga
     3301 ggcggccctg aagaagacag gcgccggtcc cgtctcctgg gaccatgcgc cggacgaccc
     3361 ggtcctgtta tggcagcgaa tcgatgccct gctgaccaac gatatcctga tcctttgcgg
     3421 cggcgtctcg gccggggatg ccgattatgt ccccggtgtg ctggaagccg ccggtgtaaa
     3481 aaaactgttc cataagatag ccatccggcc gggcaaaccc gtctggtgcg gggtgaccgt
     3541 ggatgggaag atggtctttg ccctgcccgg caatcccttc tcctgtctcg tcaatatggt
     3601 attgctgatc cggccctatc tgcaggcctg ctatggcctg cggccggcgg aaccccttgg
     3661 cctggcgctg gggacggccc gcccgaaacg ctcgccgctg gatgagttct ttccggtata
     3721 tatgcatgga tcgcccgcgc ggctcacccc cgtccccgtc aacggatcgg gtgatatccg
     3781 cctcggcatg caggccaacg gcctggccct gcatcctgcc gcctgcggcc ctctggcgga
     3841 gggtgatatc atcccctgtt attcctttgt atagtcggtc agccggatgg catcggcgat
     3901 ggcgatgttc ttgccatcca gcaggatcag gccttcctgc gccaattcat tgatgatccg
     3961 gaacacggtc tcataggtgg cgcccaccag cgaggaaaga tcctggcggc tgagggtaag
     4021 cccgatgtag ccgtcggcgg tgaccccaaa atcctcccgc aattgcagca gggcctgggc
     4081 cacccgcccc ttcacctgca tatgcgcgat gtcccgcatc ttccgctccg aggcctggag
     4141 ctcgtcggca aagaactgta acagccccag gctgaatccc tggttcacct tcaggctggc
     4201 ctggaagaat tccaggtcga aatagcaggc ttccaccggt tcgagcgcgg tggccgagat
     4261 agggtagtgg tggtcccttc ccagcccgcg atggccaaac aggctgcctg tgctggcgaa
     4321 gcggatgatg agttccttct ccgttcccca tttcttatgc accttgacgg tgccgctgta
     4381 catgaaaaag atgcctttta cctcgtcacc ttcctggaag atcatctctc cctttttgta
     4441 tgttaaattt tctctatgtg ttccgatggc cgggatccac tcgggcaggc accgcttgca
     4501 gagaaaacaa ctttgcagat cgcaggtatt gaccttcttc ttcatagaac gcaaaatagg
     4561 gttatataag gacattcctg tagcatagcg ggatgagatt gttttatatt aaaaaaataa
     4621 atatatttca gatttcatat ttttgtttca aaatttgacc acatttcgct atttttatta
     4681 catgagtaat atttagaaca aaattttcac ccccagatag tggcttttat taaatgtaaa
     4741 tatttgtaat atttatacgc cacccccttg tcttcgattg ccatccactc ggttgctgcc
     4801 attttgattt tgctgccatt tgagcccgac gtaaccaaaa cgacaaacat gcacaaattc
     4861 atcaccggca tgagcattct cctgctttct tacatttccc tgcccgtgca ggggcagctt
     4921 tccctgacgg ggcaattacg ggtccgcacc gaactacgca acggctacgg caccctggaa
     4981 accatcggca gtcaacctgc ttttctcacc tcccaacgcg cccggctcaa tttcaattac
     5041 cggtcgtccc gggtgatctt tcaggcatcg gtgcaggacg tccgcgtatg gggcgccgac
     5101 gcatcgacga tcaacaacgc cgacggctct cgccttagca tccatgaagc atggggtgaa
     5161 ctggtcctcg ccaactccca tgacagcagc ttcgggcatt cccccgtcga atatttcggc
     5221 atccgggtcg gccggcagga gctggtctat gacgatcagc ggctgctggg caacctcgac
     5281 tggctgcaac aggcccggcg tcatgatgcg atcgtcttca aactgctgaa caaaggctgg
     5341 cagaccgatc tcggcgtcgc cttcaaccag accacagacg cctttaacta caatggtaca
     5401 acctacacgc ccgccaacgt ccccgcctat gtcaaggaca gcaggggcaa tcttgccgcc
     5461 gtaccaacgg ggtatctccc cctggtgaat gccgcgggcc tcagcgcccg caccggcaac
     5521 ccctccttcg tagtgg
//
LOCUS       DATGFG010000750         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_583957, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000750 DATGFG010000000
VERSION     DATGFG010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583957"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VKQ52_14045"
     CDS             <1..546
                     /locus_tag="VKQ52_14045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88371.1"
                     /translation="IRSTLQNSSKSFIPLADEISYLSNYLSLEKLRFKEKMDYSIGVS
                     PDIDIHSALIPPMLIQPYVENSMRHGLRHKTDGKGLIRITICMSAGDLTVMIEDNGIG
                     RERASLYKTAEHIEYQSKGMTLTAERIGLMNKLYGEGIRVEIFDLRDEKEAPGGTKVI
                     IKFPLFAITSKNDLYDPDYTD"
     gene            524..1270
                     /locus_tag="VKQ52_14050"
     CDS             524..1270
                     /locus_tag="VKQ52_14050"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ88372.1"
                     /translation="MIRTILIDDEIDSIRVLRKLLETFCPQVDIIGTASGVETALELI
                     PEAKPDLVFLDIEMSQGNAFDLLNQLMPLDFQVIFVTAFDNYAIRAFKYSAIDYLLKP
                     VDIDDLRDAIGRVREKPSSKDLAQQVKVLLENVGMLQMTQQKMAIPTITGLIFVPVQD
                     IVRFEAKGNYTTIYLSNGEPIMAARTIKDYEEILPEAIFCRIHNSHIINLSRIQKYQK
                     GRGGSVIMEDGSTIEVASRRREEFLRKLLK"
     gene            1324..2895
                     /gene="hutH"
                     /locus_tag="VKQ52_14055"
     CDS             1324..2895
                     /gene="hutH"
                     /locus_tag="VKQ52_14055"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HLZ88373.1"
                     /translation="MNTNQFHYGSDVLGVGLVAGLAEGSIRGSFSASAEKRIRASQAQ
                     VAAIVSRQQTVYGINTGFGILAHTKISEEDTRILQHKILQSHSVGVGEPVPAEIARIM
                     LITKIHALARGYSGVQWETLQRLHWHLEEDVIPVVPEKGSVGASGDLAPLSHLCLPLI
                     GLGEVLYRGDRRPAREVLDQYGMSPLQLGPKEGLALINGTQFILAYAVKGVQRLHYCL
                     EAADIIGAMSLEALTGTKAPFDERLHRLRPYAGSSLVAQRLRHLLADSEIMQSHVDCG
                     RVQDPYSLRCMPQVHGASRNAWQHLRQLTEIELNSVTDNPIILEEGDTISGGNFHGQP
                     LALPLDYACLAAAEIGNISDRRCYLLLEGKWGLPLLLMRNVGLNSGFMIPQYTTAALV
                     TENKTLCFPASADSIPTSLGQEDHVSMGSISGRKLSQVLDNLEYILAIELMSACQAIE
                     FRRPLKSSGILEAAHEFVRNFVGFASEDRIFAEDIRQLHRIIHDFSFVKYCNGFARDK
                     GIALNGGFETDFGIA"
     gene            2912..>3151
                     /locus_tag="VKQ52_14060"
     CDS             2912..>3151
                     /locus_tag="VKQ52_14060"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88374.1"
                     /translation="MSTNVYQRYDPIKYKTPRGSQLNCKGWVQEAALRMLLNNLDPEV
                     AERPDELIVYGGRGKAARNFEALDNIIRLLKGLEAD"
BASE COUNT          757 a          854 c          881 g          659 t
ORIGIN      
        1 atccggtcta cgttgcaaaa ctcgagcaaa tcctttatcc cgctggccga cgagatcagt
       61 tacctgtcca actatctctc cctggaaaag ctgcgcttta aggaaaaaat ggactattcc
      121 atcggcgtct cgccagatat cgatatccat tccgctctca ttccacccat gcttatccag
      181 ccctatgtgg aaaacagcat gcgtcacggc cttcgccata agaccgacgg caaagggctc
      241 atacgtatca ccatctgcat gtcggctggc gacctgacgg tcatgataga agacaacggc
      301 atcgggaggg agagggcatc gctgtacaag accgccgagc atatcgaata ccaatcaaag
      361 ggaatgaccc tgacggccga acgtatcggt ttgatgaaca aactctacgg agaagggatc
      421 agggtggaga tcttcgatct tcgggatgaa aaggaagcgc cggggggcac gaaggtgatc
      481 attaaattcc ctttatttgc gataacaagc aaaaacgacc tatatgatcc ggactatact
      541 gattgacgat gagattgaca gtatcagagt attgcgaaaa ttactggaga ccttttgccc
      601 gcaggtagac atcatcggta cagcaagcgg tgtcgagacg gcccttgaac tgatccctga
      661 ggccaaaccc gatcttgtct tcctggacat cgaaatgtcc cagggaaacg cgttcgatct
      721 gctgaaccag ctgatgccgc ttgatttcca ggttatattc gtaacggctt tcgacaacta
      781 cgccatccgg gcctttaagt acagtgccat agattatttg ttgaagcccg tcgacatcga
      841 cgatctgcgt gatgccatcg gcagggttcg tgaaaaaccc agttcgaagg atctggcgca
      901 gcaggtgaag gttttgctcg agaatgtagg gatgttgcag atgacgcagc aaaagatggc
      961 gatccctacg atcaccgggt tgatcttcgt gccggtgcag gatattgttc ggttcgaggc
     1021 caagggcaac tatacgacga tctacctttc gaatggcgag cccatcatgg cggcgcggac
     1081 gatcaaggat tatgaggaga tactgccgga ggctatcttt tgccgtatcc acaattccca
     1141 tatcatcaat ctctcccgga tccagaaata ccagaaaggc aggggcggtt cggtcatcat
     1201 ggaggatggg agcaccatcg aagtggccag ccgccggcgc gaggaattcc tgcgtaaatt
     1261 gctgaaatag cctggcggac ggataggtaa aaaccattta tttttgttat agaaaggctt
     1321 gtcatgaata caaatcagtt ccattatggc agtgatgtgc tgggggttgg cctcgtcgcc
     1381 ggtctggccg aaggttccat ccggggaagt ttttccgcat cggccgagaa aaggatcagg
     1441 gcaagccagg cgcaggtggc cgctatcgtt agccggcagc aaaccgtcta tggcatcaac
     1501 acgggatttg gcattctcgc ccacaccaag atatcggaag aagatacccg catcctgcag
     1561 cataagatcc tgcaaagcca tagcgtcggg gtgggggagc ctgttccggc ggaaatagcc
     1621 cggatcatgc tgatcacaaa gatccatgcg ctggcccggg ggtactcggg cgtgcagtgg
     1681 gagaccttac agcggttgca ctggcatctc gaggaagatg tcattccggt ggtgccggaa
     1741 aagggctctg taggggcctc cggcgatctg gccccgttgt cccacctgtg ccttcccctg
     1801 atcggtcttg gcgaggtcct ttaccgggga gaccgcagac ccgcgcggga ggtgctggat
     1861 caatacggga tgtccccgct tcaactgggc ccaaaggaag ggcttgcgct gatcaacggc
     1921 acccagttca tcctcgcgta cgctgtaaaa ggcgtgcagc gactccatta ctgcctggaa
     1981 gcggccgata tcatcggggc catgagcctg gaagcgctta cgggcacaaa ggcgcccttt
     2041 gacgaacggc tgcatcgcct gcgcccctat gccggcagca gtctcgtcgc acaacgtctt
     2101 cggcacctgc tcgccgacag cgagatcatg caaagtcatg tcgactgcgg acgcgtgcag
     2161 gacccctatt ccctgcgttg catgccccag gtgcatggcg cttcgcgcaa tgcctggcag
     2221 cacttgcggc agctcaccga aatagaattg aactccgtca ccgacaaccc gatcatcctg
     2281 gaggaaggag ataccatcag cggcggaaat tttcatggcc agccactggc gctgccgctt
     2341 gattatgcct gcctggcagc cgcggagatc ggcaatatct ccgaccgacg ctgctacctg
     2401 ctgctggaag gcaaatgggg tctgccgctc ctgctgatgc gcaacgtcgg cctgaacagc
     2461 ggcttcatga tccctcaata tacgacggcg gccctcgtca ccgaaaacaa gaccctttgc
     2521 tttccggcca gcgccgacag catccctact tcgctgggac aagaagacca tgtcagcatg
     2581 ggcagcatca gtggaagaaa actgagccag gtgctggata acctggaata catccttgcc
     2641 atcgaattga tgagcgcctg ccaggccatc gaattccgcc gccccctgaa gagcagcggt
     2701 attctcgaag cggctcatga gttcgtgcgg aattttgtgg gatttgccag cgaagaccgc
     2761 atcttcgcgg aggatatccg gcagctgcat cgcattatcc atgacttctc ttttgtaaaa
     2821 tactgcaacg gctttgcccg ggacaaaggc attgccttga atgggggttt cgaaaccgat
     2881 tttggaatag cataataaaa aacacatcgc tatgagtacg aacgtctatc aacgttatga
     2941 cccgataaaa tacaagactc cccggggatc gcaactcaat tgcaaggggt gggtacagga
     3001 agcggccctg cggatgctgc tcaacaatct cgaccccgaa gtggccgaac ggccggacga
     3061 actcatcgta tatggcggcc gcggaaaggc ggcccgcaac ttcgaggcgc tggataacat
     3121 catccggttg ttgaaagggc tcgaagcgga c
//
LOCUS       DATGFG010000751         7206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1395852, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000751 DATGFG010000000
VERSION     DATGFG010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395852"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VKQ52_14065"
     CDS             <1..371
                     /locus_tag="VKQ52_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88375.1"
                     /translation="WVMLDYPRWFGREDVFAIRTMFWWGNTFSTTLHLKGRYKDLYLP
                     VIEQRWMELAAAGFHVGLSDDEWRHEHSTGNYGILKGMESLRGERDFLKLSAAVGLHR
                     WDDAPDLLLGMFKTLINVLF"
     gene            complement(450..830)
                     /locus_tag="VKQ52_14070"
     CDS             complement(450..830)
                     /locus_tag="VKQ52_14070"
                     /inference="COORDINATES: protein motif:HMM:NF039344.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HLZ88376.1"
                     /translation="MTKKQIFTLEYPVRCSPSILYEFLSTPAGLQEWFADKVDERDNV
                     FSFSWNGTTDRAEVTESEFEKFIRFHWMDAPAEEFFEFRIEKSEVTNQTILVIKDFAG
                     KGEVKDQSQLWSYQVKDLFHRLGN"
     gene            1246..2121
                     /locus_tag="VKQ52_14075"
     CDS             1246..2121
                     /locus_tag="VKQ52_14075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HLZ88377.1"
                     /translation="MSMRQLKITKSITNRESQSLEKYLQEIGKVELISPEEEVKLAVR
                     IKQGDQKALDKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGF
                     KFISYAVWWIRQSILQALAEQSRIVRLPLNKVGLTNRIQKAYSQLEQEFEREPSAEEL
                     AEILELDIEEVSSTLGIAARHVSMDTPLSEGEDNTLIDVLENPNAERAETNIEHRESL
                     KQEIDRSLKTLTERQKEVICFFFGIGVDHPMSLEDIGEKFNLTRERVRQIKDKAITKL
                     RSNSRSKMLRSYLGI"
     gene            2333..3655
                     /gene="ffh"
                     /locus_tag="VKQ52_14080"
     CDS             2333..3655
                     /gene="ffh"
                     /locus_tag="VKQ52_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HLZ88378.1"
                     /translation="MFESLSERLESAFKQIKGEGRITELNIAATVKDIRRALIDADVN
                     FKIAKEFTDTVREKALGQKVLTAVSPGQLMVKVVKDELVDLMGGSESELNAKGSPAVI
                     LVAGLQGSGKTTFSGKLANYLKGRKGLSPLLVADDIYRPAAIEQLKVLGEQIGVEVYS
                     EPENKNAVQIAQNAIKEARAKNKNVVIIDTAGRLAIDEAMMAEVAAIKGAVNPGEILF
                     VVDSMTGQDAVNTAKAFNDRLDFTGVVLTKLDGDTRGGAALSIKYTVQKPIKFISSGE
                     KMDTLDVFYPDRMAQRILGMGDITTLVEKAQAQFDEAEAKKLEKKIRKNQFDFEDFKQ
                     QLEQIKKMGNLKDLMGMIPGVGKAIKDIDISDDAFKGIEAIINSMTPFERNNPDVIDQ
                     SRRRRLAKGCGKDIGEINAFLKQFDQMKQMMKMMNKMPMGAMRGMGRH"
     gene            4055..4546
                     /gene="rpsP"
                     /locus_tag="VKQ52_14085"
     CDS             4055..4546
                     /gene="rpsP"
                     /locus_tag="VKQ52_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HLZ88379.1"
                     /translation="MPVKIRLQRHGSKKRPFYFIVVADARAPRDGKFIQKIGTYNPLT
                     VPASVQLDRQKALDWLHKGAQPTDTVRRILSYKGVLYLKHLLRGVKLGLFDDAAAMEK
                     FQKWHGEHEAQMKKRQEENRKARPGRRGPGGPGGAPVRKFERSERPERKAEGGAPTAS
                     AEA"
     gene            4668..5186
                     /locus_tag="VKQ52_14090"
     CDS             4668..5186
                     /locus_tag="VKQ52_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88380.1"
                     /translation="MSENYRNIGRLVAVHGLQGELVLLHRLGRKTSLKGLKTLFLEER
                     RDEMLPWFIESTRVKSDEEIFVKLEGLDTKEAARRVVQKEVWLTERDFDHFAGRSAPI
                     SMVGFRLWDGPRDLGEIIEVIEQPHQVLCRIDLEGKEALIPIHEQTLRKVDQRKREVH
                     VELPDGLLDVYR"
     gene            5284..6402
                     /locus_tag="VKQ52_14095"
     CDS             5284..6402
                     /locus_tag="VKQ52_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88381.1"
                     /translation="MNPPMPMPHRILLTLALLAVCSCAVAQDLGSDDLPSPPADEKPD
                     TARFFIEAPARRCASHYNKLELIDQRIDTARIGLISTTNYYDIRVIPGRPLGEQFGAL
                     LSAMADSGAGGRTLLVLLKRFVCYDVDTKFGSRLCVALRISFFDRHGADCRQLPNFDT
                     VVQESYSRLKGNLRKRIFATTSRAITEGMRRAMSMEPDVLSPYYTYTEVQHFDSAFRS
                     RLPLFQRDSFAEGVYLHYSSFALQKPDYTKMLADLDGDKLSNVMAATPTQASVLLSPR
                     QLYAVVWKGKAYISQGGDYYPLTRTGDDLYYTGKIRVNSEGADAIVNSTAIAAWAVST
                     ATIRMLPLVQVFGLTAKKKFLLRLDFSDGSFVPVKAVK"
     gene            6448..>7206
                     /locus_tag="VKQ52_14100"
     CDS             6448..>7206
                     /locus_tag="VKQ52_14100"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HLZ88382.1"
                     /translation="MKPSLPLPGKLPTVLLAFVLPVLLSLPALRGRAQSAANPTADPL
                     HSSAPLRQDPFIGLPLGAIQPEGWLREMLIRQKNGATGNLDVLYPQVMGKRNGWRGGD
                     GDQWERGPYWLDGLLPLAYLLHDKALIAKTTPFIEWTLKSQGADGFFGPSKDYPPEPG
                     IQRDNSRDWWPKMVMLKVLQQYYSATGDERVIRLMTNYFRYELADLPKEPLDHFTGWA
                     QARGGDNLLIVYWLYNITGDKFLLDLADLLHRQTF"
BASE COUNT         1721 a         1962 c         2026 g         1497 t
ORIGIN      
        1 cctgggtgat gctggactat ccccgctggt ttggacggga ggacgttttt gccatccgca
       61 cgatgttctg gtgggggaat accttcagca cgacgctgca tttgaagggc cggtataagg
      121 acctatacct acccgttata gaacaacgat ggatggaact ggcggcggcg ggttttcatg
      181 tcggcctctc cgacgacgaa tggcggcacg agcattccac cggtaattat ggtatattga
      241 aaggcatgga gtcgctgcgc ggagagcggg atttcctgaa actgtcggca gcggttgggc
      301 ttcaccgttg ggatgacgcg cccgatctgc tcctgggtat gttcaaaacc ctgataaacg
      361 tgctgtttta gacgataacc gtgcgggaaa gcctccgcgg ggccgggcct gtcggtggtg
      421 tgcgggaccc cggcggcgac gcaaagccac taattcccca gccggtgaaa aagatccttc
      481 acctggtaac tccagagctg gctttggtcc tttacttccc ccttccctgc gaaatccttg
      541 atcacgagaa tggtttggtt ggtcacttcc gacttttcaa tgcggaactc gaagaactct
      601 tcggccggtg cgtccatcca gtgaaaccgg atgaattttt cgaactcgct ttcggttact
      661 tccgctctgt cggtggtgcc attccaggaa aagctgaaca cattatcccg ttcgtctacc
      721 ttatctgcaa accactcctg caatcctgcc ggggtgctta aaaattcgta taagatcgag
      781 ggtgaacatc tgaccggata ctctaacgta aatatttgtt tcttcgtcat ctattgcgat
      841 tgattcttga gaccgaacat cctctccaac cttcagagcc tggaaggccg gcaaatttgc
      901 cggcatgcct caggcgcaat gatagaaaaa aaattcaacc atcaaaattt tttttccggg
      961 caaaattact atggtaaatg ggtagttttt aatggaattt taaccaatca ttgttgaaaa
     1021 tcaatccttt gttaaaaaaa ttggttacgg cctccaaatt cggtaaattg ctctttacat
     1081 ctacacctga atcagttgac gactttttga tacaagaaaa gaattattta ttcgttttta
     1141 ccaagactag atttagaatc cattatttaa gaaaatttcc atttctaaag acaatttcct
     1201 tttaagtagc cttagaacct aaaaaccctt taaagacctc tgcctatgag catgcgtcaa
     1261 ctgaagatca cgaagagtat tacgaatcgt gaatctcaga gtttggagaa gtacctgcag
     1321 gagatcggga aagtagagtt gatcagtccc gaggaagaag taaaactcgc cgtccgtatc
     1381 aagcagggag atcaaaaagc tctcgataaa ttaacgaaag cgaacctccg cttcgtggtg
     1441 tccgtagcca aacagtacca gaaccagggg ctgtcgctgc ccgacctcat caacgagggc
     1501 aatcttggtc tgatcaaagc cgctcagcgc tttgacgaaa cccgcggttt caaattcatt
     1561 tcctacgccg tatggtggat ccgtcaaagc atccttcagg cactcgccga acaatcgcgg
     1621 atcgtgcgtc ttcccctcaa caaagtgggt ctcaccaatc gcatccagaa agcctattcc
     1681 cagttagaac aggagttcga gcgtgagcct tccgcagagg aactggccga gatcctcgaa
     1741 ctcgatatcg aagaggtctc ttcgacgctg ggcatcgccg cccgtcacgt gagcatggat
     1801 acgccgcttt ccgagggcga ggacaacacg ctgatcgatg tgctggagaa tcccaacgcc
     1861 gaacgcgccg agaccaatat cgagcacaga gagtcgctca agcaggagat cgaccggagt
     1921 ctgaagaccc tgaccgaacg ccagaaagaa gtgatctgct tcttcttcgg catcggcgtc
     1981 gatcacccca tgagcctcga agacatcggg gagaagttca acctcacccg cgaacgcgtt
     2041 cgccagatca aggacaaggc gatcacgaaa ctgcggagca acagcaggag caagatgctc
     2101 cggagctacc tcgggatcta ggccgccgcc cgactgaaac cattcgaaac caacagaaag
     2161 aaatttgaat agacaaatac actgaattta cttacagcaa cgcaggaagc cataaattcc
     2221 ctaactttgt ccccccgaga gtgaatataa cttggttatg ttcacttttt ttttgggccg
     2281 gaccgggcgg ccggcgacgg caaaatctta ttaatactca attggttaca atatgttcga
     2341 gagtctttcc gagcggctcg aatcagcgtt caagcagata aagggcgaag gccgcatcac
     2401 cgaactgaac atcgcggcga cggtaaaaga catccggcgc gcccttatcg acgcggacgt
     2461 caacttcaag atcgcaaagg aattcaccga taccgtaagg gaaaaggcgc ttggccagaa
     2521 ggtgctgacg gccgtaagcc ccgggcagct gatggtcaag gtcgtcaagg acgaactggt
     2581 cgacctcatg ggcggcagcg agagcgagct caacgccaag ggcagccccg cggtcatcct
     2641 ggtggccggg ttacagggta gcggtaaaac caccttttcc ggcaaactcg ccaactacct
     2701 caagggccgc aagggcctgt cgccgctgct ggtggcagac gatatctatc gaccggcggc
     2761 tatcgagcaa ctgaaagtgc tcggcgagca gattggcgtg gaggtttaca gcgaacccga
     2821 gaacaagaac gcggtgcaga ttgcgcaaaa cgcgataaag gaagcccgcg cgaagaacaa
     2881 gaatgtcgtc attatcgata cggcaggccg tctcgccatc gacgaggcga tgatggcgga
     2941 agtagcggcc atcaaggggg cggtgaatcc tggcgagatc ctgttcgtcg tcgactcgat
     3001 gacgggccag gacgcggtga atacggcgaa agcgtttaac gatcggctgg acttcacggg
     3061 cgtcgtattg acgaaactgg atggtgacac ccggggcggg gccgccctgt cgatcaagta
     3121 taccgttcag aaaccgatca aattcatcag cagcggggag aagatggaca ccctggatgt
     3181 gttctacccc gatcggatgg cgcagcggat cctcgggatg ggggatatca ccaccctcgt
     3241 cgagaaggcg caggcccagt tcgatgaggc ggaggcgaag aagctggaga agaagatccg
     3301 caagaaccag ttcgacttcg aggacttcaa acagcagctc gagcagatca agaagatggg
     3361 taacctgaag gacctgatgg ggatgatccc cggggtcggc aaggcgatca aggacatcga
     3421 catcagcgac gacgcgttca agggcatcga ggcgatcatc aattccatga cgccgttcga
     3481 acggaacaat cccgacgtca tcgaccagag ccgccgccgg cgcctcgcga aaggttgtgg
     3541 aaaagatatc ggcgagatca acgcttttct caaacaattc gaccagatga agcagatgat
     3601 gaagatgatg aataagatgc ccatgggagc aatgcggggg atggggaggc attagggcga
     3661 cacgataaat gggaaaaatt ttggacaaaa tactcgaaac gatggatggc ggcgccgcaa
     3721 aaacgctata gcaatccgtc agacgtacta tggtggccct cagccgaacg tcggcaaagc
     3781 tccgggccct cgcttttgcc ttgttcctaa ccttcgctcc gctcggttac cccccgagcg
     3841 cctttggcgc aaaatttgat tatttacgcc tgaaaaatgg cgatttccgg tggttacgtt
     3901 aaagtttcgg gccgctggta aaaaattcgc tgcaacattc gctctttaaa gtccgaagta
     3961 ttatttttga aatgtgatag ggttggtgta atattgcact cccaaaaatt gaaaggtaac
     4021 cttatttgtt cacgcattta aatttctatt taagatgcca gttaaaatca gactgcaacg
     4081 ccacgggtct aagaaacgac cgttttactt catcgtagta gccgatgccc gtgcaccccg
     4141 tgatgggaag ttcatccaga agatcggtac gtacaatcca ttgacagttc ctgcctcagt
     4201 tcaactcgac cgccagaaag cgctcgactg gctgcacaaa ggtgctcagc ccacggatac
     4261 cgtacgccgt attctctctt acaaaggtgt tctctatctg aaacacctct tgcgtggtgt
     4321 caaattaggg ttattcgatg acgcggccgc aatggagaaa ttccagaaat ggcatggcga
     4381 gcacgaagcc cagatgaaga aacgccagga agagaacagg aaagcccgcc ccggcagaag
     4441 aggccccggc ggtcccggcg gtgcacccgt aaggaaattc gaaaggtccg aacggccgga
     4501 gagaaaggca gaaggcggtg cgcccacggc gtccgcggag gcatagatcc cggtgcaaaa
     4561 aaacagaaat tccaacagga ggttcgcgcc tcctgttttt tatttagaga ggcgcccgcg
     4621 cagcggatac gggcaaagac ccggagcttc gacgaatttc tataggtatg agtgagaact
     4681 atcgcaatat cgggcgtctc gtcgccgtac acggcctgca aggggagttg gtgctcttgc
     4741 accgcctcgg ccgcaagacc agcctgaagg ggctaaaaac gctgttcctg gaagaacggc
     4801 gcgacgagat gctcccctgg ttcatcgaga gcacccgcgt caagagcgat gaagagatct
     4861 tcgtcaagct cgagggactc gacaccaagg aggccgcgcg gcgtgtggtg cagaaggaag
     4921 tgtggctgac agagcgcgac ttcgaccatt ttgcgggcag atccgcgccc atctcgatgg
     4981 tgggcttccg cctctgggac ggcccgcgtg atctcggcga gatcatcgag gtcatcgagc
     5041 agccgcacca ggtgctgtgc cgcatcgatc tcgaaggcaa ggaggcgctc atccccattc
     5101 acgagcagac cctgcggaaa gtggatcagc gcaagcgcga agtgcatgtc gaactgccgg
     5161 atggcctgtt ggatgtgtat cgctgatgcg ggtgacccgg gcgatgcggg gggatgcgag
     5221 gggatgcgga gcgatgccgg ccgcggtggc tgatcgacac ctttttccta tttttagtct
     5281 atcatgaacc ccccgatgcc catgccccac cgcatcctgc taaccctggc cctgctcgcc
     5341 gtttgttcct gcgcggtcgc acaggacctc ggcagtgacg acctcccctc tccgccggcg
     5401 gacgagaagc cggataccgc gcggttcttt atcgaagcgc cggcgcgccg gtgcgccagc
     5461 cactataaca agctggaact catcgaccag cggatagaca ccgcccgtat cgggctgatc
     5521 agcaccacca actactacga tatccgggtt attcccggca gaccgctggg cgagcagttc
     5581 ggggccctct tgtcggcgat ggccgatagc ggggcgggag gacggaccct gctcgtattg
     5641 ctgaagcggt ttgtttgcta tgatgtcgac acaaaattcg gcagccggct ttgcgtggcg
     5701 ctgcggatca gttttttcga caggcatggt gccgactgcc gccagctgcc gaatttcgat
     5761 acggtggtac aggaatccta cagtcgcctc aagggcaacc tcagaaagcg catctttgct
     5821 accaccagcc gggccatcac ggaaggcatg cggcgggcga tgtcgatgga acccgatgtg
     5881 ctctcgcctt attacaccta tacggaagtg cagcatttcg acagcgcgtt tcgcagccgg
     5941 ctgccgctct tccagcggga cagctttgct gagggcgtct atctccacta ttcctccttt
     6001 gccttacaga aaccggacta tacgaagatg ttggccgatc tggatggcga caagctctcc
     6061 aacgtcatgg cggcgacacc aacccaggct tcggtcctgt tatccccacg ccagctgtac
     6121 gcggtggtct ggaagggtaa ggcctatatc agccaggggg gtgactatta cccgctgacg
     6181 aggacggggg atgacctgta ttacaccggt aagatccgcg tgaactcgga gggggccgat
     6241 gccatcgtga actccactgc tatcgcggcc tgggccgtat cgacggctac gatccggatg
     6301 ttgccgctgg tgcaggtgtt cgggttgacg gcgaaaaaga aattcctttt gaggctggat
     6361 ttcagcgacg gaagtttcgt accggtgaag gcggtgaaat aaaaatttgg ctaacttgtc
     6421 ggtatgaagc ccaagtcgcc tctgtctatg aagcccagct tgcctttgcc cggaaagctg
     6481 cccaccgttc tgcttgcttt tgtcttgccc gtcctgttgt ccttgcctgc tctccgggga
     6541 cgggcccaat ccgcggcgaa cccgaccgcc gacccgctcc atagcagcgc gccgttgcgg
     6601 caggatcctt ttatcgggct ccccctgggg gcgatacaac ccgagggctg gctgcgcgaa
     6661 atgctgatcc gccagaagaa tggcgccacc ggcaatctcg atgtgctcta tccgcaggtg
     6721 atgggtaagc gcaacggctg gcgcggcggc gacggtgacc agtgggagcg cggcccgtac
     6781 tggctggacg gactgctgcc cctggcctac ctgcttcatg acaaggcgct catcgccaag
     6841 acgaccccgt tcatcgagtg gacgctgaag agccaggggg cggacggctt tttcggtccg
     6901 tcaaaagact atccccccga gcccggcatc cagcgcgata acagccgcga ctggtggccg
     6961 aagatggtca tgctaaaagt gttgcagcaa tattattccg ccacgggcga cgaacgcgtg
     7021 atccggctca tgaccaatta tttccggtac gaactggccg atcttccgaa ggagcccctg
     7081 gatcatttca ccggttgggc gcaggctcgc ggcggggaca acctcctcat cgtctactgg
     7141 ctgtacaata tcaccggcga caaattcctg ctcgacctcg ccgacctgct ccaccgccag
     7201 accttc
//
LOCUS       DATGFG010000752         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_934518, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000752 DATGFG010000000
VERSION     DATGFG010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934518"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VKQ52_14105"
     CDS             complement(<1..545)
                     /locus_tag="VKQ52_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88383.1"
                     /translation="MALRSLLLSVFAFLLSTCAPAQRFAHPSIPFTKYDLDQLRANVN
                     TAPWSTGYSALASDARSQLGYNMEGPFTEVGRAPNLNNAQWISDMTAIHNLTFMYVFT
                     GDSAYARKATNMLDSWAVTNTVWSGNEAMLDIGDMAPYFVPAADILRGTFPGWTDSNT
                     IHVKNYFANVLWPQSWVPYPLR"
     gene            complement(1101..2126)
                     /locus_tag="VKQ52_14110"
     CDS             complement(1101..2126)
                     /locus_tag="VKQ52_14110"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HLZ88384.1"
                     /translation="MKKALWRERPLAVWIGISIAVIIFGFWYLMRTFVIGAYSVPTTS
                     MEKTIHLGGKVLVNKLNYRPITRGDILVFHFPEGDTVIDLPDYQSMRPYYDVIRQLGN
                     GNADSGRQIVLGNPDEYPLTVRPVYKREVYLKRCIAMPGDKLAMRDELVYIDGRLQAW
                     PPEAETYFHVVTGGHPLDEVGMKTQYGLDISNADEIHSLDSAGVYDMLLTCKAREKML
                     KDGFALRIVPDIDSSTEGVFPNDTWHHWTRDNYGPIWIPKKGASIQLTPLNYPIYERI
                     IRTYEGNKLEMRGSQIYVNGHEENSYTFKMNYYWVIGDNLHGSQDSRYWGFVPEDHLI
                     GKAWVAM"
     gene            2682..3131
                     /locus_tag="VKQ52_14115"
     CDS             2682..3131
                     /locus_tag="VKQ52_14115"
                     /inference="COORDINATES: protein motif:HMM:NF025124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88385.1"
                     /translation="MLQYNLYDIDELKKKHPSRPRNRYIADIFFRAGYIESWGRGIEK
                     MIEAFKKAGLPEPIFEELAGGLQVTLLKDIYTEEYLRKLDLSDRQIKAIIYTKEHKKI
                     SNKVYQQLFGVSRNTASNDLQQLVKAKLLISNEQKGAGAFYTIRGIA"
     gene            complement(3217..>3399)
                     /locus_tag="VKQ52_14120"
     CDS             complement(3217..>3399)
                     /locus_tag="VKQ52_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88386.1"
                     /translation="PLYEGVTKAIRRDENLYKLLAFTDVIRVGKVRECKAAIEQLKKT
                     ILLEPLREHHADQSGL"
BASE COUNT          846 a          781 c          937 g          835 t
ORIGIN      
        1 cgcagcgggt aggggaccca ggattgcggc cagagcacat tggcgaaata attttttacg
       61 tggatggtat tggagtcggt ccagccggga aatgtcccgc ggaggatgtc ggcggcggga
      121 acgaaataag gcgccatgtc gccgatatcc agcatggctt cgttacccga ccatacggta
      181 ttcgttaccg cccaggaatc cagcatgttg gtcgccttac gggcataggc gctgtcgccg
      241 gtgaagacat acataaaggt gagattgtgg atggccgtca tgtcgctgat ccactgggcg
      301 ttgttcagat tgggggcgcg gcccacttcg gtgaacgggc cttccatgtt gtagcccagt
      361 tgcgaccggg catcgcttgc gagggcgctg tagcctgtgg accagggcgc tgtgttaacg
      421 tttgcccgga gctggtccag gtcatacttt gtaaaaggta tgctgggatg ggcgaagcgt
      481 tgcgcggggg cgcaggtgga caggaggaag gcaaaaacgg ataggagtag agatctcagg
      541 gccatagcaa tcgttttggt tttttcgatg gtcaaaaata gaggtggagg ggtataagag
      601 gggaggggat ttcaaccaaa gaagaggggg aattggttga tccggccggg atcggccgat
      661 atcggcggcc gggagttgta ggcggagccc ccggcgcttg ggggccggag acggagccag
      721 cgcccgagca ccgatcgtaa atctacggta ctccgcttcg ggcaatactc gacagtaagc
      781 agttgtgcct aaagcggggt aagtttactg ataaaggggc gaaatccacc acaacaatgc
      841 ttggtgcggg cggagattcg cagcatcttt gttctgtcaa gcaaaacatc cgtacccttg
      901 acgtcccaag atccagtatc cctagaagcc gatccttaaa cggcctgttg caaccccggc
      961 ccgtttgccg tccgtatgaa aactcaatcc gattctttga aaaacctctg actagccttg
     1021 ggggtttttt cgtttctgcc cgtcgctctt tctctgcgag gagaaagaag ccggtcaatg
     1081 cgggccgggt gggaattccg tcacatggcc acccaagctt ttccgatgag atggtcttcc
     1141 gggacaaagc cccagtaccg ggaatcctgg gacccatgga gattgtcgcc gatcacccag
     1201 tagtaattca ttttgaaggt atagctgttt tcttcgtgac cattgacgta gatctggctg
     1261 ccgcgcattt ccagcttatt gccttcatag gtgcggatga tgcgttcata gatcggatag
     1321 ttcagcggcg tcagttgtat cgatgcgccc tttttcggga tccagatcgg gccgtaatta
     1381 tctctcgtcc agtgatgcca agtatcattc ggaaatactc cttcggtgga cgaatctata
     1441 tccgggacga tccgcagggc gaaaccatcc tttagcattt tttcgcgagc tttgcaggtg
     1501 agcagcatat cgtatacacc cgcactgtcg agcgagtgaa tttcatccgc gttcgagata
     1561 tccaggccgt attgcgtctt catacccact tcatcgaggg ggtggccgcc tgtgacgaca
     1621 tggaaatagg tctctgcctc cggcggccag gcttgcaggc gcccgtcgat atataccaac
     1681 tcatcccgca tggcgagttt atcacccggc atagcgatac agcgttttaa gtagacctcc
     1741 cgtttgtaca ccgggcggac ggtcaatgga tattcatcag ggttacccag cacgatctgt
     1801 ctgccggaat cggcgtttcc gtttcccagt tgccggatca catcataata gggccgcatg
     1861 gattggtaat ccggcaggtc tatgacggtg tctccctcgg ggaaatggaa gacgaggata
     1921 tctcctctgg tgatcgggcg atagtttagc ttgttcacca atactttacc ccccagatgg
     1981 atggttttct ccatggacgt agtgggtacg ctataggccc cgatcacaaa tgtcctcatg
     2041 aggtaccaaa agccaaaaat aatgaccgcg atactgatgc ctatccatac ggccagtggc
     2101 cgctcccgcc aaagcgcttt tttcatagaa gatttattaa atgagtgaag tcattgtgct
     2161 tttcgtgacg accggtctaa aaatatagaa aaaatgggaa agggggtagg acaatagcct
     2221 cgccatcggt ggttgccata tcaggaccgt tggcggccca tccagaacac tagacaacat
     2281 acggtcccgg tgagaatggc gcataagaaa acgctgttgc ccgagtcgta gccggtgtgc
     2341 caagtggcac cggctctccc ggtgctgtat ctggccttga cgaccgtccc ggtcagtcgc
     2401 atgtagctgg cgaagagcaa taggagcaag gtggtggtgg cgaggagatt tttggcgatt
     2461 ctcatagaag aggaatgcag tttgaggaga agatacaaaa aaagatgtaa ggaactcccc
     2521 cggcaaggcc tatgagtttg ccgattcttt gtgcaaaatt ttgaaaatga agtaaagacg
     2581 gtgataaata gacgaagcgt actattgaat gataggtaaa attggcttta gtaaggccgt
     2641 caggaaagag tcaatacacg cgtagcaaac atattattaa tgtgttacaa tataatttat
     2701 atgacataga tgaacttaaa aagaagcacc cttcccgccc gagaaaccga tatatagcgg
     2761 acatattttt cagggcaggc tatattgaat cctgggggcg gggcattgaa aaaatgattg
     2821 aagcattcaa gaaggccgga cttcccgagc ctatatttga ggagttagca ggtgggcttc
     2881 aagttacgct gttgaaagat atttacacgg aggaatattt gcgaaaactg gatttgagtg
     2941 atcggcaaat caaagccata atttatacaa aagaacataa gaaaatatcc aataaggtct
     3001 accagcaact atttggcgtt tctcgcaata ctgcttctaa cgatcttcag cagctcgtga
     3061 aggctaaatt gctaatatcg aatgagcaaa aaggagcggg tgcattttat actattaggg
     3121 gaattgcata ataattgcac aaattgcaca tttgttgcct tttttgtaag cctaaaggcc
     3181 cgttaatcgg tcgaggtagc cgacaactgt caagcgttat aaaccgcttt gatccgcgtg
     3241 atgttctctt aatggctcaa gtaaaatcgt ttttttcagc tgttcgatcg cagccttaca
     3301 ttccctgacc tttcccactc gaattacgtc tgtgaatgcg agcagtttgt aaagattttc
     3361 gtccctgcga atggctttgg tgacaccttc gtataaagg
//
LOCUS       DATGFG010000753         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1036745, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000753 DATGFG010000000
VERSION     DATGFG010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036745"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VKQ52_14125"
     CDS             <1..239
                     /locus_tag="VKQ52_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88387.1"
                     /translation="AHCPWHQVFWIVDGGETVFFGGDVAPQLQQMKSRFVAKYDYDGR
                     KAMELRQQWWEQGQKEGWTFLFYHDVKTPTWRAF"
     gene            complement(443..859)
                     /locus_tag="VKQ52_14130"
     CDS             complement(443..859)
                     /locus_tag="VKQ52_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88388.1"
                     /translation="MKKILSVLVILCWLTLPAFSQVRQQQPGAGVIETMKIGYITTQL
                     NLTPQEAEKFWPIYRQYSAEVRQAYANYRVDNNEIGLEETLLNIKKKYSVEFVKAIPP
                     PKINDFWRVEKNFNDILRREQIRRQQMQNRRFPPGG"
     gene            complement(868..1704)
                     /locus_tag="VKQ52_14135"
     CDS             complement(868..1704)
                     /locus_tag="VKQ52_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88389.1"
                     /translation="MNGKNDISEELRLLSELIATISRETPYRVADDYFPDLAARVLLR
                     VKTQHKPLAFNVPDGYFDGFAEGVLARIKSGAGKASGPGKPVPEGVEAELASLSPLLA
                     QLRQAETYRIPEGYFEEISPILAVAKNKNPYTVPEEYFHRLPVEIEEKVLEPVTVEEE
                     EQARVITLGAHRSNWWKYSAAAVVAGLIFTVGWLRLYVPGGHRTTSPDIVAASLPRVS
                     DQDLQRFLADQDTTLAQPTSNLAMQDFSENDLKTLLGDVPDGELKQYMEEHGGANDIA
                     TN"
     gene            complement(1736..2281)
                     /locus_tag="VKQ52_14140"
     CDS             complement(1736..2281)
                     /locus_tag="VKQ52_14140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ88390.1"
                     /translation="MTIQDTELLLQFRDPETKERAFTAIIRKYQEKLYWHIRRMVVEH
                     EDANDVLQNVFIRVWNGLENFREDSQLYTWLYRIATNECLSYLEQQKRKSALPLDETE
                     SGLSNKIVADKYFDPNKLEWKLQLAIQQLPEKQRVVFTLRYYDEMPYEEMSRVLDTSE
                     GALKASYHHAVKKIEDYILNH"
     gene            complement(2286..>2861)
                     /locus_tag="VKQ52_14145"
     CDS             complement(2286..>2861)
                     /locus_tag="VKQ52_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLZ88391.1"
                     /translation="EDIGLMKMLPGMTVIVPCDYAQTKAATMAIADYQGPVYLRFGRP
                     VWPIFTTALPFEIGKAQVLSEGTDVSIFACGHLVWKAIEAGRILEEKGLSVEVINLHT
                     VKPLDTAAILKSVQKTKCAVTAEEHNIIGGLGDAVAQTVARNFPIPVEYVGTNDTFGE
                     SGTPEELLKKYGLDTPNIVAAAEKVIARKNK"
BASE COUNT          595 a          754 c          777 g          735 t
ORIGIN      
        1 gggcgcactg tccctggcac caggtcttct ggatcgtcga tggtggcgag accgttttct
       61 ttggcgggga tgtcgcgccg cagctgcagc agatgaagag ccggtttgta gcgaagtatg
      121 attatgatgg gaggaaggcg atggagttga ggcagcaatg gtgggaacag gggcagaagg
      181 aggggtggac ctttttgttc tatcacgatg ttaaaacgcc cacgtggcgg gcattctagc
      241 ggtgcgagat taaaatggca ttaaacgcta aagtcgggcc ttcggccatg cggccggggc
      301 cgcataagaa aaaggcctgc cctggaaaga gccggccgtt gctaacctat gaaaaacacc
      361 aagttcaaat atcgtatttc aggcaattgc cgataaaatt tttcggtgaa tggacgaaat
      421 gtgagggtca gcgaattgtt aattaacctc ccggtggaaa acggcggttc tgcatctgtt
      481 gcctgcggat ctgttcacgg cggagaatgt cattgaagtt cttttccacc cgccagaaat
      541 cgttgatctt ggggggaggt atcgccttga cgaattccac gctgtacttt ttcttgatat
      601 tgagcagggt ctcttccagg ccgatctcgt tgttatcgac gcgatagttg gcataggcct
      661 gccgcacttc cgccgaatac tgtctataga tgggccagaa tttttccgcc tcctggggag
      721 tgagattcag ttgggtagtg atatacccta ttttcatagt ttcgatgacc cccgcgccgg
      781 gctgttgttg ccgcacctgc gaaaacgccg gtagggtcag ccagcagagg atcaccaata
      841 cggaaagtat ctttttcatt gttactgcta attcgttgca atgtcatttg cccctccatg
      901 ctcttccata tattgcttca attctccgtc gggtacatcc ccgagtaagg tcttcaggtc
      961 gttttcgctg aaatcctgca tcgcaagatt gctggtaggt tgcgccagcg tagtatcctg
     1021 atcggccagg aagcgctgca gatcctggtc gctcactctg ggcaggctgg cggcgacgat
     1081 atcgggtgac gtcgtacgat ggccaccggg gacgtacaac cgaagccagc cgacggtaaa
     1141 gatcaggccc gccacgacgg cagccgccga atatttccac cagttgctcc tgtgtgcgcc
     1201 gagggtgatc acccttgcct gctcttcttc ttctacggtg acaggttcaa gcactttttc
     1261 ttctatctcg accggtaacc ggtgaaagta ctcctccgga acggtatagg ggttcttatt
     1321 tttggcgacg gccaggatgg gcgagatctc ctcgaaatag ccctccggga tccggtaggt
     1381 ctccgcctgc cgaagctggg ctaaaagggg ggaaagcgac gccaattctg cttcaacacc
     1441 ctccggaacc ggtttacccg ggccggacgc ctttccggcc cccgacttta tccgggcgag
     1501 gacaccctcg gcaaaaccgt cgaaataccc gtcaggaaca ttgaatgcca gtggtttatg
     1561 ttgagtcttt acccggagta gcacccgggc tgcaaggtct ggaaaataat catccgccac
     1621 ccggtagggg gtctcgcggc tgatcgtggc gatgagctca ctcagcaacc tcaattcttc
     1681 cgatatgtca ttcttcccgt tcatctcttt tcagacccct accgttaacc aaagtttaat
     1741 gattgagaat gtaatcctct atttttttaa ctgcatgatg atacgaagcc ttcaacgccc
     1801 cctcggaagt gtccaggacc cggctcattt cctcgtaggg catctcgtca tagtatcgaa
     1861 gcgtaaatac aactcgttgt ttttcaggaa gttgctggat cgccagctga agcttccatt
     1921 ccagcttatt gggatcgaag tacttgtccg ccacgatctt gttactcaga ccggactccg
     1981 tctcatcaag cggcagggcg gacttccgtt tctgctgttc gagatagctc aggcattcgt
     2041 tggtggctat ccggtagagc caggtataca gctggctgtc ttccctgaaa ttctccaggc
     2101 cgttccagac acgtataaag acgttctgta atacgtcgtt agcgtcctcg tgttccacta
     2161 ccatccgccg gatatgccaa tacagctttt cctgatattt cctgatgatg gcggtaaagg
     2221 cccgctcttt tgtctcagga tcccggaatt gcaaaagcag ctcggtgtct tggatggtca
     2281 ctatactatt tgttttttct tgcaattacc ttttcagcgg cagcgacgat attgggtgta
     2341 tccaggccat attttttcag caattcttcg ggtgtaccgc tttccccgaa cgtatcgttg
     2401 gtgccgacat attcgacagg gatcgggaaa ttcctggcca ccgtctgtgc gacggcgtca
     2461 ccgaggccgc cgatgatatt atgttcttcg gccgtaaccg cacattttgt cttttgcacc
     2521 gacttcagga tcgcggccgt gtcgaggggt tttaccgtat gcaggttgat cacctctacg
     2581 gagaggcctt tctcttccag gatcctgccg gcttcgatgg ccttccatac gaggtgaccg
     2641 caggcgaaga tggacacatc ggtcccttcg gagaggacct gcgccttgcc gatctcgaag
     2701 ggcagggcgg ttgtgaagat cggccatacg ggacggccaa aacgaaggta taccggtccc
     2761 tggtagtcgg cgatggccat ggtggcggcc ttcgtctgtg cgtaatcaca gggcacgata
     2821 acggtcatcc ccggcaacat cttcatcagg ccgatgtctt c
//
LOCUS       DATGFG010000754         4856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_813170, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000754 DATGFG010000000
VERSION     DATGFG010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813170"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="cmk"
                     /locus_tag="VKQ52_14150"
     CDS             complement(<1..546)
                     /gene="cmk"
                     /locus_tag="VKQ52_14150"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HLZ88392.1"
                     /translation="MKKIIITIDGWSSCGKSTLAKQLAKHLHYVYIDSGAMYRAITLY
                     FLRHHIDWTIPAAVGEALKHITLEFVTNHKSGQTEMWLNGENVEYVIRDLVIAEKVSE
                     VAAVRAVREFAVAGQQEMGKKKGIVMDGRDIGTVVFPHAELKIFMTADEKVRVQRRFK
                     ELYEKNPNITLEEVKQNLSHRD"
     gene            complement(614..3139)
                     /locus_tag="VKQ52_14155"
     CDS             complement(614..3139)
                     /locus_tag="VKQ52_14155"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HLZ88393.1"
                     /translation="MDNNFSAQVKEIISFSREEALRLGNDFIGTEHLLLGLIREGDNT
                     AVRILKSFNVDLYELRKEVEMAVKDKTGKNIANINSLPLTKQAEKVIRVTVLEAKALK
                     SPTVETEHLMLSILKNKENIATQILNQFDVDYDLFRNELGVVKSNDVRSEYAEENDDD
                     FDEEKKYSQQKARSAGNAKSKTPVLDNFGRDITRLAEMGALDPIVGREEEIERVSQIL
                     SRRKKNNPILIGEPGVGKTAIVEGLALRIVQRKVSRVLFDKRVISLDLAALVAGTKYR
                     GQFEERMKAIMNELEKNRDVILFIDEIHTIVGAGGASGSLDASNIFKPALARGELQCI
                     GASTLDEYRMYIEKDGALDRRFQKVMVDPPSVEETIQILNNIKSKYEDYHNVTYSDDA
                     IDACVKLSDRYMTDRLLPDKAIDVLDEVGARVHLKNINVPQNIVDLEKKIEDVKNEKN
                     KVVKSQKFEEAASLRDTEKRLQEELEKAKAEWEEESKHKRYPIDEENIAEVVSMMTGI
                     PVKRMVQAETEKLRNMAIDMRGMVVGQDDAITKVVKAIQRNRVGLKDPKKPIGTFIFL
                     GPTGVGKTELARALARYMFDSEDSLIRIDMSEYMEKFTVSRLIGAPPGYVGYEEGGQL
                     TERVRRKPYSVILLDEIEKAHPDIYNILLQVLDDGQLTDGLGRKVDFKNTLIIMTSNI
                     GVRQLKDFGDGVGFATAARTQNADENNKAVIEKALKRTFSPEFLNRIDDVVIFNSLNK
                     EHIFQIIDILMKGVLKRLTNLGFTLELTDEAKSFLADKGYDQQFGARPLHRAIQKYLE
                     DPLAEEILNMNIKNGDILIADLDKENSKIKFTLKEKAKEKSEA"
     gene            3233..3613
                     /locus_tag="VKQ52_14160"
     CDS             3233..3613
                     /locus_tag="VKQ52_14160"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLZ88394.1"
                     /translation="MQVKIDTREKFHAITIREPILAANMTEDLERCLLPLLQKEVKNV
                     VLILKDISEVDDAAAECLVRIQQNFYDESASFVICELTPGVEKRLEDNGLLELMNVTP
                     TESEAWDIVHLEEVERELLNGPDL"
     gene            3708..4466
                     /locus_tag="VKQ52_14165"
     CDS             3708..4466
                     /locus_tag="VKQ52_14165"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HLZ88395.1"
                     /translation="MPLEIKQFLQKNLPGAIGLLPTAELHLRPLGGGSINAAYQLSTN
                     HNSRWFCKFNNTTTFPDLFVKEDRGLALLRRQHVIRVPATVACLQADGHQLLVLEWIE
                     QGPRTDGFWRQGLPGHAGPKNSMSGKPSNVGPSASRFWRLFGEQLATLHRLTQPEFGL
                     AEDNYMGALPQANTPSADWIDFFIHRRLEPQIHLAARQGLLDGAAIRHFERLYQRLPH
                     LFPPEPPALLHGDLWNGNFLCDATGRPVLIDPAV"
     gene            4522..4806
                     /locus_tag="VKQ52_14170"
     CDS             4522..4806
                     /locus_tag="VKQ52_14170"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HLZ88396.1"
                     /translation="MMGLRPNRPWRFLGHRSIDLAMTTLFGGFEKPFYEAYHHHYPFP
                     PDHRRQWDICNLYPLLIHLNLFGPDLRRSVVPRSSSVVPFAALGYILQIF"
BASE COUNT         1034 a         1488 c         1096 g         1238 t
ORIGIN      
        1 gtcccggtgg gaaagattct gttttacttc ctcgagagtg atattcgggt tcttctcata
       61 caattccttg aaccgccgct gtacccgtac cttctcgtcg gcggtcataa agatcttcag
      121 ttcggcatgc gggaacacca ccgtcccgat atcccggcca tccatgacga tccccttctt
      181 cttccccatc tcctgctgcc cggccaccgc aaattcccgc accgccctca ccgccgccac
      241 ctcgctcacc ttctcggcga tcaccaggtc gcggatcaca tactcgacat tctccccgtt
      301 cagccacatc tccgtctgac cactcttgtg gttggtaacg aattcaagcg taatatgctt
      361 caacgcctcc ccaacggcgg caggaatggt ccagtcgata tgatgccgga ggaaataaag
      421 cgtaatagcc cggtacatcg cgccgctgtc gatatagaca taatggaggt gcttggccaa
      481 ttgctttgcc agcgtactct tgccacagga cgaccagccg tcgatagtga taatgatctt
      541 cttcatttcg gctacaaaaa aacaaaaaaa agccgcactt tgcagcgcgg ctccatttat
      601 tctaataaat tatctaggct tcagactttt cctttgcctt ctcctttaaa gtgaatttga
      661 tcttcgaatt ctccttgtcc aggtccgcga tcaggatatc cccgttcttg atattcatgt
      721 tcaggatctc ctcggccagg ggatcttcca ggtatttctg gatggccctg tgcagcggtc
      781 tcgccccgaa ctgctgatca taccccttgt ccgccaggaa gctcttggcc tcgtctgtca
      841 gctcgagcgt aaagccgagg ttggtcagcc gcttcaacac tcccttcatc aggatatcga
      901 ttatctggaa aatatgctcc ttgttcagtg aattgaagat gacgacatcg tctatgcggt
      961 tcaggaactc agggctgaac gtacgcttca acgctttctc gatcacagcc ttgttgttct
     1021 cgtccgcatt ctgcgtccgc gcggccgttg caaacccgac cccgtcccca aaatccttca
     1081 actgtcgtac cccgatattg gaggtcatga tgatcagggt attcttgaag tcgaccttac
     1141 ggccaagacc atccgtcaac tgcccgtcat ccagcacctg caacaggata ttgtagatat
     1201 ccgggtgtgc cttctcgatc tcatcgagca ggatcaccga gtagggtttc cggcgaaccc
     1261 gttccgtcaa ttggccgcct tcttcatatc cgacatatcc gggaggcgca ccgatcagcc
     1321 ggctcaccgt gaatttctcc atgtattcgc tcatatcgat ccggataagc gaatcctccg
     1381 aatcgaacat ataccgggca agcgcacggg ccaattcggt cttaccaacc ccggtaggtc
     1441 cgaggaagat aaaagtgcct atcggcttct tcggatcctt caggccgacc ctgttacgct
     1501 ggatagcttt caccaccttt gtaatggcat catcctgacc gacgaccatg ccccgcatat
     1561 cgatcgccat attcctcaat ttttccgtct cggcctgtac catccgtttt acggggatac
     1621 cggtcatcat gctcaccacc tcggcgatat tctcctcgtc gatgggatag cgtttgtgct
     1681 tgctttcctc ttcccattcc gccttcgcct tttccagttc ttcctggaga cgtttctccg
     1741 tgtcccggag cgaagcggct tcttcgaact tctggctctt caccaccttg ttcttctcgt
     1801 tcttcacgtc ttcgatcttc ttctcgagat cgacgatatt ctgcggaaca ttgatattct
     1861 taaggtgtac tcttgcacct acctcgtcca gtacgtcgat ggccttgtcc ggcagcaggc
     1921 ggtccgtcat ataccggtcg ctcagcttca cacacgcatc gatggcgtca tcggagtaag
     1981 taacattgtg gtagtcctcg tacttcgact tgatattgtt gaggatctgg atggtctctt
     2041 ccacgctggg gggatcaacc atcaccttct ggaaccggcg gtcgagggct ccgtcctttt
     2101 cgatgtacat ccggtactcg tcaagggtag aggctccgat gcactggagc tcgccacggg
     2161 ccagggcagg cttgaagatg ttggaagcat cgagcgatcc gctcgcacca cccgcaccca
     2221 cgatcgtatg gatctcatcg atgaacagga tcacatccct gttcttctcc agttcgttca
     2281 tgatcgcctt catcctctcc tcgaactgac cccggtattt cgtaccggca accagcgcgg
     2341 caagatcgag ggagatgaca cgcttgtcga acagtacccg cgacacctta cgctgtacga
     2401 tccggagggc caggccttcc acgatcgccg tcttacccac accgggctca ccgataagga
     2461 tggggttgtt cttcttgcgg cgggaaagga tctgcgagac cctttcgatc tcttcctccc
     2521 ggccgacaat gggatccagc gcccccatct cggcaagacg ggtgatatcc cggccaaaat
     2581 tatcgagcac tggcgtcttg ctcttggcat tgcctgcaga acgtgccttt tgttgcgaat
     2641 acttcttctc ctcgtcgaaa tcgtcatcat tctcctcggc gtattcactg cgaacatcgt
     2701 tgctcttcac aacaccgagt tcgtttctga atagatcata atccacgtcg aattgattta
     2761 agatttgtgt agcaatattt tctttgttct tcaggatcga gagcatcagg tgttcggtct
     2821 ctaccgtagg gcttttaagg gctttggctt caagaacagt cacgcgtatc actttctccg
     2881 cctgtttagt cagcggcaga ctattgatgt tggcgatatt cttaccggtc ttatccttta
     2941 ctgccatctc cacttcctta cgcaattcat acaggtccac attgaaactc ttcaatatgc
     3001 gaacagccgt attatcgccc tcacggatca accccagcaa taagtgctcc gtaccgataa
     3061 aatcgttccc cagtcgcaaa gcttcctccc tgctgaacga tatgatctcc tttacctggg
     3121 ctgaaaaatt attatccatt tttaagtaat tgagctttat acaataataa cgaatatttt
     3181 tgacttccga ttccgccact ttattaacgg ccgttaataa tcattagtgt atatgcaagt
     3241 caaaattgat accagagaaa aatttcatgc cattactatc cgggaaccca ttcttgctgc
     3301 taatatgaca gaagatctgg agcgatgttt actgcccctc ctccaaaaag aagttaaaaa
     3361 cgtggttctc atactaaaag acatctcaga ggtggacgac gctgctgctg aatgcctggt
     3421 tcggatccaa cagaacttct atgatgaaag cgcctccttc gtcatttgcg aactcacacc
     3481 aggagtagag aaaaggctgg aagacaatgg ccttttggaa ctcatgaacg taacacccac
     3541 cgagagcgaa gcctgggata tcgttcatct ggaagaggtg gaaagagagc tgctaaacgg
     3601 tccggacctc tgatcagacc cccatcctgc cggttgccta caggctcctg caggtttgac
     3661 ctcacctaat cgggacaacg gacagaaagt cagctacacg ctcggaaatg cctctggaaa
     3721 tcaaacagtt tcttcaaaaa aacctcccgg gagccatcgg cctcctaccc actgccgaac
     3781 tccatttgcg gcccctgggc ggcggctcca tcaacgctgc atatcagtta tctacgaatc
     3841 ataacagccg gtggttctgt aagttcaata atactaccac ttttcctgac ttatttgtaa
     3901 aggaggatag gggattggcc ttattgcgcc ggcaacacgt gatccgcgtg ccggcaaccg
     3961 ttgcctgcct gcaggccgac gggcatcagc tgctggtgct ggaatggatc gaacagggcc
     4021 cgcgcaccga tggtttctgg cggcagggtt tgcctggaca tgctgggccg aaaaatagca
     4081 tgtccggcaa accatctaat gtcgggcctt cggccagccg cttctggcgg cttttcggcg
     4141 aacaactcgc cactctgcac cggctcactc agccggaatt cggcctcgcc gaagacaatt
     4201 acatgggcgc gcttccccag gccaacaccc cctccgccga ctggatcgac ttcttcatcc
     4261 accgccgtct cgaaccccag atccacctcg ccgcccgcca gggcctcctg gacggggcag
     4321 ccatccggca cttcgagcgc ctctatcagc gcctgcctca cctctttcca cccgaacctc
     4381 cggccctcct ccacggcgat ctgtggaacg gtaacttcct ctgcgacgcc accggccggc
     4441 ccgtcctcat cgaccccgcc gtctagcttt gtttgccggg ctgcgttctc tggaatgtaa
     4501 cgcaccccgg caaagaatct aatgatgggc cttcggccaa accgcccgtg gcggtttctg
     4561 ggacatcgca gcatcgacct ggccatgacc accctcttcg gcggcttcga aaagccgttt
     4621 tatgaggctt accatcacca ctacccattt cccccggacc accgccgcca gtgggatatc
     4681 tgtaacctat accccctcct catccatctc aacctcttcg gaccagacct tcgtcggagc
     4741 gtcgtccctc gctcctcctc ggtcgtaccc ttcgctgcgc tcgggtatat cttacaaata
     4801 ttttgaacac aatccggggc tattaacccg cgtttacttc gcaatcccta tattcg
//
LOCUS       DATGFG010000755         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1270549, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000755 DATGFG010000000
VERSION     DATGFG010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270549"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /gene="manA"
                     /locus_tag="VKQ52_14175"
     CDS             <1..944
                     /gene="manA"
                     /locus_tag="VKQ52_14175"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HLZ88397.1"
                     /translation="KDMLSIQVHPSKHNAELEFAAENKKGVALNAADRNYKDDNHKPE
                     LMVALSEFWLLHGFKPEENLAETLESAPELAFLLPLFEKDGYQRLYRAVMEMPQAEVN
                     RVLEPLLQRILPPYKDGRLRKHEEDFWAARAALTYEQPGGIDRGIFSIYFFNLLNLHP
                     GEAIFQDAGLPHAYLEGQNVEIMANSDNVLRGGLTPKHVDVPELLKHVRFEATHPHIL
                     PEEYVAGRIAVFHTPAPDFELSKISLLKGELLTIRAHSPEIFIVMEGKVGVIEPGADP
                     FSRKSGEAFLAFHGAKFELNAQADTIVYRASVPAGQQ"
     gene            complement(969..1439)
                     /gene="rnhA"
                     /locus_tag="VKQ52_14180"
     CDS             complement(969..1439)
                     /gene="rnhA"
                     /locus_tag="VKQ52_14180"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HLZ88398.1"
                     /translation="MSNELIMYTDGASRGNPGPGGYGTLLFWGDHRKELSGGYRYTTN
                     NRMELMAVIAGLEALKKGGLKVTIYSDSQYVVKAVEQGWLKNWIATDFKGGKKNKDLW
                     LHFHELAKKFTLRFVWVRGHADNPYNNRCDQLATAAADNGPLHVDKVFEAENGR"
     gene            complement(1457..3097)
                     /locus_tag="VKQ52_14185"
     CDS             complement(1457..3097)
                     /locus_tag="VKQ52_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HLZ88399.1"
                     /translation="MNAETLDFNKNEDAMRQSLSQLRQRLEKIALGGGRKAMEKQREK
                     NKLAPRERIRYLVDEGSSFIELGAFAGYELYEDQGGCPAGGTVAGIGYVSNRQCIIIA
                     NDQTVKAGAWFPITGKKNLRLQEIAMENHLPVIYLVDSAGVFLPMQDEIFPDKEHFGR
                     IFRNNARMSAMGITQIAVVLGPCVAGGAYLPIMSDETLMVEGNGSIFLAGPYLVKAAI
                     GEEVDAETLGGAVTHTEISGIADYKFKTEQECFLQVKKIMSRLGHPSGPSIFDRIDPA
                     PPPHRAEELYGILPADATKPYDMVDIITRIVDGGAFEQFKEDYGKTILCGYARIEGWA
                     VGIVANQRKIVKNKKGELQMGGVIYNDSADKAARFIMNCNQKKIPLVFLQDVTGFMVG
                     SRSEQAGIIKDGAKMVNAVANSVVPKITIIIGNSYGAGNYAMCGKAYDPRFIYAWPTA
                     RIAVMGGEQAAKTLLQIQVGALKAKGQEIDPAEEKKLLEEIKGRYERQTSPYYAAARL
                     WVDAIIDPADTRRVIAEGLYAAAHNREMPEFRTGVFQV"
     gene            3243..>3546
                     /locus_tag="VKQ52_14190"
     CDS             3243..>3546
                     /locus_tag="VKQ52_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HLZ88400.1"
                     /translation="MERYYSTGVYLLLSRCQRILFGWLPFSVGDVLYLTVIIWLIVAL
                     VRTIRTFIRREAGLAWFLSFFRRTLFICLLVYVLFNGLWGLNYDRKGISFQLQLHVQ"
BASE COUNT          676 a          943 c         1048 g          879 t
ORIGIN      
        1 tgaaagatat gctgtccatc caggtgcatc cctcgaaaca caatgccgag ctggaatttg
       61 cggccgagaa taaaaagggt gttgcgctga atgcagccga tcggaattat aaggatgata
      121 accataagcc ggagctgatg gtggcgctga gcgaattctg gctcctgcat gggttcaagc
      181 cggaggagaa ccttgcggag acgctggaat cggcgccgga actggccttt ctccttcctt
      241 tgttcgaaaa ggacggctac cagcgattat accgggcggt gatggaaatg ccgcaggcgg
      301 aagtgaaccg ggttctggag ccactgctgc aacgcatact cccgccgtat aaggacggtc
      361 gattgaggaa gcatgaagag gatttctggg cggcgcgggc cgcactgacc tatgagcaac
      421 cgggcgggat cgaccggggc attttctcta tctatttttt caacctgctg aacctgcatc
      481 cgggcgaggc gatcttccag gatgcgggat taccgcatgc ttacctcgag gggcagaatg
      541 tcgagatcat ggctaattcg gacaatgtat tgcggggcgg gctgacgccg aagcatgtcg
      601 atgtccccga attgttgaag catgtccggt tcgaggcgac gcatccgcat atcctgccgg
      661 aagaatatgt agcggggcgt attgccgtct ttcatacgcc ggcgccggat ttcgaactga
      721 gcaagatcag cctgttaaag ggggaactgc tgaccatccg ggcgcattcc ccggagatct
      781 ttatcgtgat ggaggggaag gtgggggtca tcgagccggg ggcggaccct ttcagccgga
      841 agagtggaga ggcgttcctg gcttttcacg gggcgaaatt cgagttgaat gcgcaggcgg
      901 atacgatcgt gtatcgggcg tcggtgccgg cggggcagca gtaaaaggga atgggccgat
      961 tagagcaact aacgaccgtt ctctgcttca aaaactttgt ccacatgcag gggaccgttg
     1021 tcggcggcgg ctgtagccaa ctgatcgcag cggttgttat agggattgtc cgcgtgccct
     1081 cttacccata caaagcggag ggtaaatttt ttggccagct cgtggaagtg cagccagagg
     1141 tccttatttt tttttcctcc cttgaaatcg gtggcgatcc agttctttag ccagccctgt
     1201 tcgacggcct tgaccacata ctggctgtcg gagtagatgg tcaccttcag gccgcccttt
     1261 ttcagggctt cgagaccggc gatgacggcc atcagctcca tgcgattgtt ggtagtgtaa
     1321 cggtagccgc cggacagttc cttgcggtgg tcgccccaga agaggagggt cccgtagccg
     1381 ccggggccgg ggttaccccg tgaagcaccg tccgtgtaca tgatcagctc attgctcatg
     1441 gggcaaatat aggatatcaa acctggaata cgccggtcct gaattccggc atctcgcggt
     1501 tgtgtgcggc ggcatacaag ccttctgcga tgaccctgcg ggtgtcggcc ggatcgatga
     1561 tggcgtccac ccatagccgt gcggcggcgt agtaagggga agtctgccgt tcatatcgtc
     1621 ctttgatctc ctccagcagc ttcttttctt ccgcggggtc gatctcctga ccctttgctt
     1681 ttaaggcgcc gacctgtatc tgcagcagtg ttttggccgc ctgctcacca cccataacgg
     1741 cgatgcgggc tgtcggccat gcatagatga aacgcggatc ataggctttg ccgcacatgg
     1801 cataattgcc cgcgccatag gagttgccga tgatgatggt gatcttgggc acgacggaat
     1861 tggcgacggc attgaccatt ttggcgccgt ccttgatgat cccggcctgt tcgctgcggg
     1921 agcccaccat aaagccggtg acgtcctgca ggaagacgag gggtatcttt ttttgattgc
     1981 agttcatgat gaagcgggcg gccttgtcgg cgctgtcgtt ataaatgacg ccgcccatct
     2041 gcagctcccc ttttttattc tttactattt tccgctggtt ggccacgatg ccaaccgccc
     2101 agccttcgat gcgggcataa ccgcagagga tggtcttgcc atagtcttcc ttgaattgtt
     2161 cgaaggcacc gccatccacg atgcgggtga tgatatcgac catatcgtag ggctttgtcg
     2221 cgtcggcggg aaggatgcca tacaattctt cggcgcggtg tgggggcggc gccggatcga
     2281 tgcggtcgaa gatggatggg ccggagggat gtccaagccg gctcatgatc ttcttcacct
     2341 ggaggaagca ttcctgctcc gtcttgaact tatagtcggc gatgccggag atctccgtgt
     2401 gtgttacggc gcctcccagt gtttcggcat ctacctcctc accgatggcg gcttttacga
     2461 ggtagggacc ggccaggaag atggagccgt tgccttcgac catcagggtc tcgtcgctca
     2521 tgatgggcag gtaggcgccc cctgccacgc aggggcccag cacaacggct atctgggtaa
     2581 tgcccatggc gctcatccgg gcattgttac ggaagatccg gccaaaatgt tctttgtcgg
     2641 gaaatatctc atcctgcatc ggaaggaaga cgccggcgct gtccacgaga tagatcacgg
     2701 gcagatgatt ttccatcgcg atctcctgta gccgcaggtt cttcttccct gtgatgggaa
     2761 accaggcgcc ggcttttacc gtctggtcat tggcgatgat gatgcattgc cggttgctga
     2821 cataaccgat gcctgccacc gtgccgccgg ccgggcagcc accctggtct tcgtataatt
     2881 catagccggc aaaagcgccc agttcgataa aggacgaacc ctcatccacc aggtatcgaa
     2941 tacgctcacg aggggccaac ttgttctttt cccgctgttt ttccattgct ttcctgccac
     3001 cgcccagcgc gatcttctcc agccgttgtc tgagttggct gagagactgg cgcatcgcgt
     3061 cctcgttctt gttaaaatcc agggtttccg cattcatatg cctgactttc tgtgcaattt
     3121 aacatggttg atctaataat tggagtattt ttagcgggat gtccgtccga cgtaaaataa
     3181 tatggatatg cctgcttgtg cttgcggcgg gcattaagtt attgtccttt tttccggcgg
     3241 ccgtggaacg ctattattct acgggtgtat acctcctgct ctcacggtgt cagcggatcc
     3301 tctttggctg gctgcccttc agcgttggtg atgttctgta cctgaccgtt attatctggc
     3361 tgatcgttgc gttggtccgt acgatcagga cctttatccg ccgggaggcc ggcctcgcct
     3421 ggttcctttc ttttttccgc cggacccttt ttatctgtct cctggtctat gtcctgttca
     3481 acggattgtg ggggctgaac tatgacagga agggcatctc ctttcagttg caactgcatg
     3541 tacagc
//
LOCUS       DATGFG010000756         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_553962, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000756 DATGFG010000000
VERSION     DATGFG010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553962"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VKQ52_14195"
     CDS             complement(<1..1138)
                     /locus_tag="VKQ52_14195"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HLZ88401.1"
                     /translation="MNRLVLFTLFSGLFLPVVVSAQELSAEERQRCEQEAKQVTILRD
                     KWGVPHVYGKTDAAAVFGLMYAECQEDFSRVEKNYLEMLGRQAETYGESYLYTDVMMR
                     LIYDSAQAIADYERSPGWMHQLLDAFADGVNYYLYKHPSTKPLLLHRFQPWYALMFTD
                     GSVSATSTGGVRQEEIRSFYSQPVALGMGHPRKAREVSDIEKMVLGDRGIGVPANGGE
                     GAGNDDGVKHVVRKDGGQEPGDQGGGRPEDQDGDRPKGQDRGGPEDRNGGEPGGWNGG
                     EWGGWLQGADERGSNGFALAGFRTASEATELYINPHVPFYFRMECQVVSDEGLNAYGA
                     VTWGQFFVYQGFNAHCGWMHTSSYADVADLYAEKVKQQDKGWVYE"
     gene            1165..2061
                     /gene="fdhD"
                     /locus_tag="VKQ52_14200"
     CDS             1165..2061
                     /gene="fdhD"
                     /locus_tag="VKQ52_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HLZ88402.1"
                     /translation="MPISSEIGTSSPGITLLPVQKIAAHRLQETTDPIAIEEPLEIRL
                     RYGPTAKKQEQSISITMRTPGHDEDLALGWLYTEGILPAGIANIAGISNLAGTTTAGT
                     TNLQTTIPSPNTITVTLPESIPPDLSRSQRHSFTTSSCGVCGKTSLEAIRTITPQQKD
                     TPFSIPAGLLYPLPALLREGQDIFDATGGLHAAGLFDRHGNLIILREDIGRHNAVDKV
                     IGHTLLHQGLPLSETILLLSGRACFELIQKAAMAGIRIVAAVGPPSSLAIRHAEDTGI
                     TLVGFLRQQRFNIYSGSSRIEV"
     gene            2165..>2586
                     /locus_tag="VKQ52_14205"
     CDS             2165..>2586
                     /locus_tag="VKQ52_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88403.1"
                     /translation="MSNEKNQQQHDDNTRTDLPAAENPDDFTGLHTGHIRHKAVGLPA
                     IVSSFRYAIGEAGLARGLKALARVNQKGGYDCPSCAWPDPDDERSGIAEYCENGAKAV
                     AEEATTKKLTAAFFAEQQHSVAALAGLSDYHLGSKGRIA"
BASE COUNT          578 a          997 c          561 g          450 t
ORIGIN      
        1 actcatagac ccagcccttg tcctgttgtt ttaccttctc cgcatagaga tcggccacat
       61 ccgcatagct ggaagtatgc atccagccgc agtgggcgtt gaacccctgg taaacaaaga
      121 actgccccca ggtaaccgcg ccataggcat tcaacccttc gtcactcacc acctggcatt
      181 ccatccggaa atagaacggt acatggggat tgatataaag ctcggtagcc tcggatgcgg
      241 tcctgaaacc cgccagggcg aacccattgg acccccgctc atccgcgccc tgtagccatc
      301 cgccccactc gccgccattc cagccgcccg gctcgccgcc attccgatcc tccggaccac
      361 ccctatcctg acccttcggt ctgtcaccat cctggtcctc cggtctaccg ccaccctgat
      421 cccccggctc ctgtcctcca tcctttcgta cgacatgctt caccccatcg tcgttcccgg
      481 ccccttcgcc gccattcgcc ggcaccccga tccccctgtc ccccaatacc atcttctcga
      541 tgtcggagac ctcacgcgcc ttgcggggat gccccatccc aagcgctaca ggctggctgt
      601 agaaactgcg gatctcttcc tgccgcaccc cgcccgtgct cgtggccgat acgctcccat
      661 cggtgaacat cagcgcatac cagggttgga atcgatgtaa caacaagggc ttcgtcgaag
      721 gatgtttata gagatagtaa ttgacgccgt cggcaaaagc atccagcaac tggtgcatcc
      781 agccgggact ccgctcatag tccgctatcg cctgcgcact gtcataaata agccgcatca
      841 tcacatccgt ataaaggtag ctctccccat aggtctccgc ctgccgtccc agcatctcca
      901 ggtagttctt ctccacccgg ctgaaatctt cctggcattc ggcatacatc aacccgaata
      961 ccgcggccgc atcggttttg ccgtagacat ggggaacgcc ccatttatca cgcaggatcg
     1021 tcacctgctt cgcctcctgt tcacaacgct gccgttcctc ggcggaaagc tcctgcgcgg
     1081 aaaccaccac cggcaaaaaa agtcccgaaa acagggtaaa gagtaccagt ctattcatcc
     1141 ccctaattta acgtaatttt gaatatgccc atctcatccg aaataggtac atcctcaccc
     1201 ggcatcaccc tgctgccggt gcaaaagatc gcggctcacc gcctgcagga aacaaccgac
     1261 cccatcgcga tcgaagagcc cctggagatc cgcctccggt acggcccaac cgccaaaaaa
     1321 caggagcaaa gcatctccat taccatgcgc acccccggcc acgacgaaga cctggccctt
     1381 ggctggctgt acaccgaagg catcctcccc gccggaattg ccaacatcgc cgggatctcc
     1441 aacctcgccg gcaccaccac cgccggcacc accaacctcc aaaccacgat accctccccc
     1501 aataccatca ccgtcaccct tcccgaatcc atcccccccg acctcagccg ctcacaacgc
     1561 cactccttca cgaccagcag ctgcggggtc tgcggcaaaa cctcccttga ggccatccgg
     1621 acaattacgc cacaacaaaa agataccccc ttctccatcc cggccggcct cctctatccc
     1681 cttccggccc tcctgcgcga aggccaggat atcttcgacg ccaccggcgg gctccacgcc
     1741 gcaggactct tcgaccggca cggcaatctc atcatccttc gggaagacat cggtcgccac
     1801 aacgccgtcg acaaagtcat cggtcacacc ctccttcatc agggactccc cctttcagag
     1861 accatcctcc tgctcagcgg ccgcgcctgt ttcgaactca tccaaaaagc cgccatggct
     1921 ggcataagaa tagtagcagc agtagggcca ccctccagcc tggccatccg tcacgccgaa
     1981 gacaccggca ttaccctggt gggcttcctg cggcagcaac gattcaatat ctattccggc
     2041 tccagccgga tcgaagtctg agctcccggc cccaaacact ggccccaacc actggccaca
     2101 accaccggcc caaactgccg gcgccaactc atagcccccg tatccacgtc aaaaacagac
     2161 acccatgagc aacgaaaaga accagcaaca acacgacgat aacacccgta ccgacctccc
     2221 cgctgccgaa aaccccgacg acttcacagg cctccacacc ggccatatcc gccataaagc
     2281 cgtcggcctg cccgccatcg tctccagctt ccgctacgcc atcggcgagg ccggcctcgc
     2341 gcgcggcctc aaagccctcg cccgcgtcaa ccaaaaaggc ggctatgact gccccagttg
     2401 cgcctggccc gaccccgacg acgaacgctc aggcatcgcc gaatactgcg agaacggcgc
     2461 aaaagccgtt gccgaagaag caacgacaaa aaaactcacg gcggcattct ttgccgaaca
     2521 acaacattcc gtcgccgccc tcgccggcct ctccgactac cacctgggca gcaagggccg
     2581 catcgc
//
LOCUS       DATGFG010000757         5819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_506194, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000757 DATGFG010000000
VERSION     DATGFG010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506194"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..591)
                     /locus_tag="VKQ52_14210"
     CDS             complement(145..591)
                     /locus_tag="VKQ52_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88404.1"
                     /translation="MSDIARAVPVNLGNLSGYLRLRPEDKLPKRKTLEKHYQNLKVAF
                     SSELRLLSQQELRSGQEFSYETRGVEELPDEYGLRNEGEFFGKRGGQGLAPGERGLAP
                     GEQGPPLEQRVLSIESAMREMTEKMVEMEGLLRRLLERGGKEDAGS"
     gene            916..1299
                     /locus_tag="VKQ52_14215"
     CDS             916..1299
                     /locus_tag="VKQ52_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88405.1"
                     /translation="MSDKTWFSKILAWFNKWLNSRIDSVPPSSIREEIQQLPGAVVDY
                     GKNSLSKPGIKNAKTLAETRKINAEATAIELDNELKKQKIALDMAKISLLQRYGKTPL
                     LLPWDDEDSQKLLTNGEEGTSSPPT"
     gene            complement(1341..1769)
                     /locus_tag="VKQ52_14220"
     CDS             complement(1341..1769)
                     /locus_tag="VKQ52_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88406.1"
                     /translation="MPTDLKSGIRLKGIELLNSAINLPPVAEVAFTDFSYTINVESKA
                     DGANQLIFVVITVEVRSDEYSFVLGVLSISCIYTHVHFEKIVKSTSGRKVDIPQALKD
                     ILNEMSLSTMRGVMFATFKGTFLHNAFLPMIDPELLHGKL"
     gene            complement(1753..2217)
                     /locus_tag="VKQ52_14225"
     CDS             complement(1753..2217)
                     /locus_tag="VKQ52_14225"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ88407.1"
                     /translation="MKNNSARKQSSRVVRELLSEITPVERMQTSTKMMLAARIDDLLA
                     ARGLGKSEFAAKVNKNPSEITKWLGGTHNFTIDTLTEIAVAFDLSVAELFAPKPLDIV
                     DRVQVVVRAERAQPALPYHTPLREMMAGRNGDPRERNKNSKEVTISRHANRS"
     gene            2430..2972
                     /locus_tag="VKQ52_14230"
     CDS             2430..2972
                     /locus_tag="VKQ52_14230"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HLZ88408.1"
                     /translation="MAKSKIELIVPDEIVMSKILLIRGRKVMIDRDLAELYGVTTKQM
                     NQQVKRNLKRFPEDFMFQLTTEEKEEVVTFCDHLDTLKYSPVLPYVFTEHGAVMLASV
                     LNSDRAIEVNIQIVRIFTRMREILMTNQEVLLKLEQLERKVNGHDEDIQVIFAYLKQL
                     LDPPKEPRPRIGFRRKDEQD"
     gene            complement(3039..4772)
                     /locus_tag="VKQ52_14235"
     CDS             complement(3039..4772)
                     /locus_tag="VKQ52_14235"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88409.1"
                     /translation="MKKNQFIAYITVLAALLTLGGCKKDSFLNRSPLSDISPETFFTS
                     ETDFQLYCNQYYNALPVQTLLRADDQSDDKANATLNSLLAGTYTIPATADTTNDLIGT
                     AAYISQGGLLNGSGTNWSFALNRRCNFFLANYQKANLSDSIKNEYVAETLFFRANDFW
                     KKVKAFGDVPYINKYITDTSKSVLYGTRMPHQQVMDSVLNDMNFAIAHLPYVAAQTGR
                     INKYVALALKARICLWEGTYRKYWSNAGDPTAYLQGAVDAAEQLMNSGQFGIYSTGNP
                     TSDYYNLFIQDELLGNKEAILPMRYLSSILMNGIDRSLGEAGDGYSKNFVRNFLCTDG
                     LPTALSPLYKGDDSIDEEILNRDPRLVQQIATRGFNLLNGDLITVPRIATTNTSTGYQ
                     CIKGRSGSIAAWNANASTYDFFIFRYAETLLIEAEAKAEQGQCTQQVLDKTINLLRDR
                     VGMPHMIIANLVKDPNSEFPALPVLLDEIRRERRVELGSEGFRFDDLHRWHVGSLINN
                     PETILGIKVLPPFAAEYTYNISGVVLDANHYVRVYPGITARQWDDKMYLYPIPIQELT
                     LNPGLKQNPGW"
     gene            complement(4802..>5819)
                     /locus_tag="VKQ52_14240"
     CDS             complement(4802..>5819)
                     /locus_tag="VKQ52_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ88410.1"
                     /translation="FTWEKVQTKNIGLDYSFLRDRLNGSFDYFLTAIKDILVAGPQLP
                     DVLGTAAPSGNYADVKSHGYELSINYRDKALNGKLSYSVTFTWANSSNVEVTRYQNNP
                     TKSLSDFYVGSHIGDFWGFRNQGFYATDAEAQAVNNSALAGYKWLAGDIKYLDVNKDG
                     KIDYGKGTVSSPGDQQLIANTTPHNKIGLNLNVSYQGWDFTSFFQGVLKHDFYPNEYV
                     FYAFRDDEYSIPSQFALNYWTPTHTNSFFPRLRFAGGGNEQKQDKYILNAAYLRVKQL
                     TLGYTLPRAATNRLHLQRVRFYVTGQNLFTFTPLNKNYDPEVVGFNTYPLNKSVSFGL
                     QVGL"
BASE COUNT         1507 a         1764 c         1217 g         1331 t
ORIGIN      
        1 aacgcccatt tcatcggcat ccgcgccgag atcgccaccc tcgtctcctc cgaactgtcc
       61 cggatgctcg ccgacccgca attgtcgctc ctcatcatca caaagcaaaa tcaataaatt
      121 catttatcgt aacctaaagc gctctcaact cccggcatct tccttgcccc ctctctccag
      181 cagtctcctt agcaaacctt ccatctctac catcttttcc gtcatttccc tcatcgcaga
      241 ttcgatcgac aacacccgtt gctccagcgg tggtccctgc tctcccggcg ctaacccccg
      301 ctctcccggc gccaacccct gcccacctcg tttcccgaaa aattctccct cgttccgcaa
      361 cccatactca tccggcaact cctccacacc cctcgtctca taagaaaact cctgcccact
      421 ccgcaactcc tgctgcgaca acaaccgcaa ctcactgcta aacgccacct tcaaattctg
      481 ataatgcttc tccagcgtct ttcgcttcgg caacttatcc tccggccgta gccgcagata
      541 cccactcagg ttccccaggt tgacgggcac agccctggca atatcactca attgcagccc
      601 atattcttca ataaggaagc cgatcgcctt atttaacttt ataacaagtt catatgtagc
      661 attttgtgcc aatcgaaaaa tcctcccgca gggagagcga aagattttta aatctgtttt
      721 gaattatttt tatatttgtg gactaagtag accgcattgg aggcaaaaca aaaaatgccc
      781 aaccgctgac ctctcgaaag agaggatcat tgcgaccgga accgtctgca ataaaggttg
      841 gctggaaaca aatgacagcc gctaagataa actattggtc gtataagacc gcatcaaaac
      901 cagacaaaaa tgagtatgtc cgacaaaact tggttctcca aaatcctcgc ctggttcaac
      961 aaatggctca actcccggat cgattctgtc cccccatctt ccatccgcga ggaaattcaa
     1021 caactccccg gcgccgtggt cgactatgga aaaaacagcc tcagcaaacc cggtatcaag
     1081 aacgcaaaaa ccctcgcgga aacaagaaag atcaacgcgg aagcaacggc catcgaactg
     1141 gacaacgaac tcaaaaaaca aaagatagct cttgacatgg ccaaaatcag cctgttgcag
     1201 cggtacggta aaaccccctt actcctccct tgggacgacg aagactctca aaaactcctc
     1261 actaatggag aagaaggaac atcctcaccc cccacctgac ttgcccgtca agccacgtcc
     1321 ccaccgtata gtttgacatt tcacaacttc ccatggagca gctccgggtc gatcatcgga
     1381 aggaacgcat tatgcagaaa cgtcccttta aaagtagcaa acataacccc ccgcatagtc
     1441 gacaacgaca tctcgttgag aatgtccttc aacgcctggg gaatatcaac cttgcggccg
     1501 gatgtcgatt tgactatctt ctcgaaatgc acatgcgtat agatacaact tatcgacaaa
     1561 actcctagca caaaactata ctcatcactc cgcacttcca ccgtgataac gacaaatatc
     1621 agttgattgg ctccgtctgc cttactctcc acgttgatcg tgtagctaaa atcagtaaac
     1681 gccacctcgg caacaggtgg cagattgatc gcactgttta ataactcgat ccccttcaac
     1741 cgaattccgg atttaagatc tgttggcatg tcgggaaata gtaacttctt ttgaattttt
     1801 attgcgctcc ctgggatccc cattccggcc tgccatcatc tctctcagcg gcgtatgata
     1861 gggtagggcc ggttgtgccc gctccgcccg caccacaact tgcaccctgt ccacaatatc
     1921 cagcggcttc ggagcaaaca actccgccac cgatagatca aaagccaccg caatctccgt
     1981 aagggtatcg atagtgaaat tatgcgttcc gccaagccac ttcgtaatct ccgaaggatt
     2041 cttattcacc ttcgccgcaa attcactctt gcccagcccc ctcgcagcaa gcagatcatc
     2101 gatccgcgca gccagcatca tcttggtact cgtctgcatc ctttccacag gagtaatctc
     2161 actcaacaac tctcgtacaa cacggctgct ctgcttcctc gctgaattat tcttcattgt
     2221 tgtcaaaaat taaatttccc gataatcgta atcccacttc tttggagctt cacctttaga
     2281 taaaattacc taacaaagat acattttttc acttaaataa ctatttttat cttaaaagta
     2341 aataaatccc ccctataaga agagacccaa gtcgaacccg ttcaatagca gaagagtcga
     2401 ttattagtaa atcccttaaa ctgaatctta tggcaaagag caaaattgag ttgatcgtcc
     2461 ctgacgagat agtgatgagc aaaatactac tcatccgggg tagaaaagta atgatagaca
     2521 gagacctggc tgaactatat ggggtaacga ccaaacaaat gaatcagcag gtaaaacgta
     2581 acctgaaacg cttcccggaa gactttatgt tccagttgac aacagaggaa aaagaggagg
     2641 tggtcacatt ttgtgaccac ctcgacacgc tgaaatactc gcctgtttta ccctatgttt
     2701 ttacagaaca cggcgctgtc atgttagcta gtgtgttgaa tagcgacaga gccattgaag
     2761 tcaacatcca gatcgttcgc atcttcaccc ggatgcggga aatactaatg accaaccagg
     2821 aggttctctt aaagctggaa cagctggaaa gaaaggtgaa tgggcacgac gaagatatcc
     2881 aggtcatttt tgcctacttg aagcaattgc tcgatccacc aaaggaacct cgcccccgca
     2941 tcggcttccg caggaaggac gaacaagact aaaaaaaaac cggcccgggg aacccccgga
     3001 ccggcatata aaaccatcaa aagaacttcc gtaaaaacct accaccccgg attctgcttc
     3061 aaacccggat tcaacgtcaa ctcctggata ggtatcgggt acagatacat cttatcatcc
     3121 cactgccgcg ccgtgatccc cgggtacacc cgcacataat gattcgcatc caacacaacc
     3181 ccgctgatat tatacgtata ctccgcggca aatggcggca acaccttgat cccaaggatc
     3241 gtctccggat tgttgatcaa cgacccaaca tgccaccggt gcagatcgtc gaagcggaac
     3301 ccttccgacc ccaactccac ccgacgctcc cgcctgatct catccagcaa caccggcaac
     3361 gccgggaatt cggaattcgg gtccttcacc aggttcgcaa tgatcatatg cggcatccct
     3421 acccggtccc gcaacaggtt gatcgtctta tccaacacct gctgcgtaca ctgcccctgc
     3481 tccgccttcg cctcggcctc gatcagcaac gtctctgcat atcggaatat gaagaaatca
     3541 taggtcgacg cattcgcatt ccacgccgcg atactgccgc tcctcccctt gatacactgg
     3601 taccccgtcg acgtattggt agtggcgatc cgcggcacgg tgataagatc cccgttcagc
     3661 aggttgaaac cccgcgtcgc aatctgctgc accaaccgcg ggtcccggtt caggatctcc
     3721 tcgtcgatcg aatcatcccc cttatacaac gggctcaacg ccgtcggcaa cccatccgta
     3781 caaaggaaat tccgcacaaa attcttgctg tacccatccc cggcctcgcc aagactccgg
     3841 tcgatgccgt tcatcagaat agaagacagg taccgcatcg gcaggatcgc ctccttgttc
     3901 ccaagcagct catcctggat aaacagatta taatagtccg atgtcggatt gcccgtgctg
     3961 tagatgccga actgcccgga attcatcagc tgctccgcgg catccaccgc cccctgcagg
     4021 taagccgtgg gatcacccgc attcgaccag tacttccggt aggttccctc ccacaggcag
     4081 atcctcgcct tcagcgccag cgccacatac ttgttgatcc tccccgtctg ggccgccaca
     4141 tacggcagat gcgcgatcgc aaaattcata tcgttcagca ccgaatccat cacctgctgg
     4201 tgcggcatcc gcgtcccata caacaccgac ttcgacgtgt ccgtgatata cttgttgata
     4261 tacggcacat ccccgaacgc cttcaccttc ttccagaaat cattggcccg gaagaacagg
     4321 gtctccgcca catactcatt cttgatggag tcggaaaggt tcgccttctg gtaattcgcc
     4381 aggaagaaat tgcacctccg gttcagcgca aacgaccagt tcgtcccgct cccgttcagc
     4441 agtccgccct ggctgatata cgccgccgtc ccgataagat cgttcgtcgt atccgccgtg
     4501 gcgggaatgg tatacgtccc cgcaagcagg gaattgagcg tcgcattggc cttgtcatcc
     4561 gattgatcat ccgcccgcaa aagggtctgt acgggcaacg cgttataata ctggttacaa
     4621 tatagctgga aatccgtctc actggtaaaa aaggtctccg gactgatatc gctcagcggg
     4681 ctcctgttca gaaaactatc cttcttgcac ccgcccaggg tcagaagcgc cgcaagcacc
     4741 gttatataag ctatgaattg attctttttc atgttgataa aaattaaaat ggacacgcga
     4801 attacaaacc aacctgcagg ccaaaagaaa ccgacttgtt cagcgggtag gtattgaacc
     4861 caaccacctc cgggtcatag ttcttgttca gcggcgtgaa cgtgaacagg ttctgccccg
     4921 tgacatagaa ccgcacccgc tgcaggtgca accggttggt agccgccctc ggcagggtat
     4981 accccagcgt cagctgcttc acccgcagat acgcggcgtt gaggatatac ttgtcctgtt
     5041 tctgctcgtt gccgcccccg gcaaaccgca gccgcgggaa aaaggaattg gtatgcgtcg
     5101 gcgtccagta gttgagcgca aactgcgaag ggatactgta ctcatcatcc cggaaggcat
     5161 agaacacata ctcattggga tagaaatcat gcttcaacac cccctggaaa aagctggtaa
     5221 agtcccaacc ctggtaactc acatttaagt taagtccaat cttattatgc ggcgtcgtat
     5281 tggcgatgag ctgctggtct cccgggctgc tcaccgtccc tttcccataa tcgatcttcc
     5341 catccttgtt cacatcgaga tacttgatat ccccggccag ccacttgtag cccgccagcg
     5401 cggagttgtt cacggcctgc gcttcggcat cggtggcata aaaaccctgg ttcctgaagc
     5461 cccagaagtc cccgatatgg ctgcccacat aaaagtcgct gaggctcttc gtcgggttgt
     5521 tctggtatcg cgtgacttct acattggaag agttggccca ggtgaacgtc acactgtacg
     5581 atagcttgcc gttaagcgcc ttgtcgcggt aattgatgct cagctcatac ccatgcgatt
     5641 tgacatcggc atagttcccc gaaggagcgg ccgtgcctaa tacgtcaggt aattgcggtc
     5701 cggcaaccag tatatccttt attgccgtca ggaaataatc gaagcttcca ttcagtctgt
     5761 cccgcaggaa ggaatagtcc aaaccgatgt tcttcgtctg caccttctcc cacgtaaag
//
LOCUS       DATGFG010000758         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_472370, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000758 DATGFG010000000
VERSION     DATGFG010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472370"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /locus_tag="VKQ52_14245"
     CDS             complement(<1..1695)
                     /locus_tag="VKQ52_14245"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HLZ88411.1"
                     /translation="MLTICTKKYWSLGILLLLFATAPARASIKSWQKEGDAVNFTLDI
                     GSMIIRVCRPDLIEVKYTMLPRMQQKNSLVVVNRFGGETGFTVTETATSVVITTKRLK
                     ITIDKGNNAITYADAGGTSLLAEYGSDNKTMQGTVVANIRTYSCSTTFWSPRDEGLFG
                     LGCHPLDSLAINYKGRNQDLLIKYLTGAIPVLMSTKGYGLLWDNYSASKFIGEDPSKY
                     KYVSESGNQIDYYFFYGPDFDHIVSLYRDATGRAPMFGKWAYGLFQSEDRYKTQNEVL
                     AAGAGYRNAHIPVDVVVQDWYYWEPLPIGSHVMNPGRYPDPKAMVDSLHNEHLHAMIS
                     IWPVFGTGTNNFNALKAMGGLTSITWDNVVTHTFDTYYDAHNPAARQLYWEQARDSLI
                     KRYGWDAWWIDQCEPDNGSLLDARRNAQFFTGKGIDYFNTYSLMHTDGVYQGWRKDIP
                     GKRAFFLARQSFAGEQRNATTLWSSDIECTWHSLQDQIPQGINACASGIPYWTSDIGG
                     YHYHWQAPDWSRPEFRELFTRWFQFGAFCPVFRIHGKGERALYSRNWDEGTRSVLLT"
BASE COUNT          483 a          720 c          564 g          493 t
ORIGIN      
        1 ggtcagcaac acgctccgcg tcccctcatc ccagttgcgc gaatacaagg cccgctcccc
       61 ctttccatgg atccggaaga cgggacaaaa cgccccaaac tggaaccatc gagtaaacaa
      121 ctcccggaac tccggccgcg accagtccgg cgcctgccaa tgataatggt acccgccgat
      181 gtccgaggtc cagtaaggga tccccgacgc acaggcattg atgccctggg ggatctggtc
      241 ctgcagacta tgccaggtgc actcgatatc cgaggaccag agcgtcgtcg cattacgctg
      301 ctcccccgca aacgactggc gggcgagaaa gaacgcccgt ttccccggga tatccttgcg
      361 ccagccctgg tatactccat cggtgtgcat cagcgaatag gtattgaaat agtcgatccc
      421 cttgccggta aagaactgcg cattccgccg cgcatcgagc agggacccgt tatccggctc
      481 gcactggtcg atccaccagg cgtcccagcc atagcgtttg atgaggctgt cgcgcgcctg
      541 ctcccaatac aattgccgcg ccgcggggtt atgcgcgtcg taataggtgt cgaaagtatg
      601 cgtcaccaca ttgtcccagg ttatcgaggt cagaccgccc atcgccttca gcgcgttgaa
      661 attattcgtt ccggtcccga acaccggcca gatggagatc atcgcatgca ggtgctcgtt
      721 atggagcgag tcgaccatcg ctttcgggtc ggggtagcgc ccgggattca tcacatggga
      781 gccgatcggt aaaggctccc aataatacca gtcctgcacc accacatcca cggggatatg
      841 cgcattgcga taacccgcgc cggcggccag cacctcgttt tgtgtcttat agcggtcctc
      901 gctctggaac aggccatagg cccacttgcc gaacatcggt gcgcgacccg tcgcatcgcg
      961 gtacagcgaa acgatatgat cgaaatccgg accgtaaaag aaataatagt cgatctggtt
     1021 cccgctctcc gatacatatt tgtatttcga cgggtcttcc ccgatgaact tcgacgcaga
     1081 ataattatcc catagcaacc cgtatccctt cgtcgacatg agcaccggga tcgcccccgt
     1141 gagatatttg atcagcaggt cctggttgcg gcccttatag ttgatggcca gcgaatccag
     1201 cggatggcag ccgaggccga agagcccctc gtcccgcggc gaccagaagg tggtggaaca
     1261 ggaataggtg cggatattgg cgaccaccgt accctgcatg gtcttgttat cgctgccgta
     1321 ctcggcaagc agggaggtgc cgccggcgtc ggcataggtg atggcgttat tgcctttgtc
     1381 gatagtgatc ttcaggcgtt tggtcgtgat caccaccgat gtggcggtct cggtcaccgt
     1441 aaatccggtc tcgccgccga agcggttgac caccacaagg gagttcttct gctgcatccg
     1501 cggcagcatc gtgtacttga cttcgatgag atcgggccgg cagacgcgga tgatcatgga
     1561 gccgatgtcg agcgtaaaat tcacggcgtc accctctttc tgccaggatt ttatggacgc
     1621 ccgtgccggc gccgtcgcaa acaacaaaag caaaatacca agggaccagt atttcttagt
     1681 gcaaatcgtt agcatacaat gacttttgag aagtttatca tattgggacg gctaaagata
     1741 aggcattttt cacaaattta tgcaaccgat tgcagcatgg attaggcggc ccatcagcgc
     1801 ggactcctag tcatcccagc cgcgccatca caaacatttg gccgcccgtg cgcgtggccc
     1861 atcccccttc acaaactttc ctatgccccg cacttgcatt ccccttattt atcccttaca
     1921 tttgataagc cccccctgcc tcgcaccgac cctcctgatt cgcaccggcc cctttgcttc
     1981 gcattgacct tctacttcgc actgaccttc tgcttcgcat cgaccctcct atccaaacca
     2041 tccttcctgt caacacttcg aatcgtccca cacttgtcta ttgtatttgt taaccaaaat
     2101 ctaccttcat gagcacgatc acacacctcc gtcaatgggt cctggccctg ctattgctta
     2161 tcgttttcgc cgcctgccgc aaagaaacct ccacccgatc cgcagccaac acaacctccg
     2221 ccgatctcgt catcaccccc gccggcctca tgccccgcag
//
LOCUS       DATGFG010000759         5257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_851230, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000759 DATGFG010000000
VERSION     DATGFG010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851230"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1030)
                     /locus_tag="VKQ52_14250"
     CDS             complement(68..1030)
                     /locus_tag="VKQ52_14250"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HLZ88412.1"
                     /translation="MKVLCGLFGKTRHAWYDRQWREQQDIFTDEIILQAVQKTRQQLP
                     RVGTRKLQELIAPELASHGIEVGRDYLFDLLAQHKLLIRQRKRKAYTTDSRHWMRKYS
                     NLTGQLVIHRPEQLWVSDMTYIRIQNQWGYLSLITDAWSRKIMGFCLRSDMLAEGCVT
                     ALQMALDNRSYEEQPLIHHSDRGAQYCCKQYVDVLTAAGIAISMTENGDPYENALAER
                     INGIIKGEFNLYTSQLNFEQTCQKVQESITAYNEIRPHGSCDNLTPNQAHQTLTPMKK
                     RWRSYPRLETAPAAGPLPSQSKGRPLVYGQDKIQYLSPERQLSP"
     gene            complement(1054..1599)
                     /locus_tag="VKQ52_14255"
     CDS             complement(1054..1599)
                     /locus_tag="VKQ52_14255"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ88413.1"
                     /translation="MSLENRKNELPALKKAGGHYDKLAVQKIVEAIEGGMRRRDICAI
                     YGVSRSTIADWMRNYGSECYKISKQGHLNNAQKRSMIRAIREGRMTLQEAKLSYNMKS
                     YTAILNLLRQDGENSDLSGAMKPKTNAAIGQEDAEKKALQKALEEAQLKIKALNTLID
                     VAEDQFKIPIRKKPGAKQSKP"
     gene            1706..2203
                     /locus_tag="VKQ52_14260"
     CDS             1706..2203
                     /locus_tag="VKQ52_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88414.1"
                     /translation="MPEQWNNFFVMVGSGAAALAGLIFVAMSINHEIIIRNTTHKNRA
                     INMLTGFTAIFMASSFALIGNQDPGVLGFEWLALWFVATVIFIRGYVIAIRSGMSFIG
                     LNTPRLAGGTICYVAEVIGAIFLIRGYSSGLYIAATAIIVLFAFLISGAWLLMIGIYE
                     DRAKR"
     gene            2308..2628
                     /locus_tag="VKQ52_14265"
     CDS             2308..2628
                     /locus_tag="VKQ52_14265"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ88415.1"
                     /translation="MRRDIFQAIADPTRRAIIALIALQAMTPNAIAENFNTTRQAVSK
                     HLRILSECDLVKQEYQGREIYYSLEIEKMKEIDKWLDQFRKIWETRFTQLDEVLSTMK
                     KQEK"
     gene            2625..3209
                     /locus_tag="VKQ52_14270"
     CDS             2625..3209
                     /locus_tag="VKQ52_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLZ88416.1"
                     /translation="MSGVQIKRKIIVTEFITLDGVIEAPGGNETPHPHGGWQSKYSSP
                     EAGKYKVEELASVDALLLGKTTYEAFAAYWPGQTGAGFADPINKYPKYVVSRSLQKAE
                     WNNSHILRDVAKDVAALKKSDGGDILVYGSATLVKALLHHDLIDELRLMVYPLSIGGG
                     LRLFDDNREAKKFVLKHSRTTDNGVLILEYQPIG"
     gene            complement(3232..3615)
                     /locus_tag="VKQ52_14275"
     CDS             complement(3232..3615)
                     /locus_tag="VKQ52_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ88417.1"
                     /translation="MKKVTGIGGIFFKCKDPNKMKEWYKTHLGLDTNQYGANFEWRED
                     ADPTKKGSTVWSPFAETTKYFDPSTKDYMINYRVENLEALVEQLKNEGVTIVDKIEAF
                     DYGKFVHIIDVEGNKVELWEPADTE"
     gene            complement(3651..4793)
                     /locus_tag="VKQ52_14280"
     CDS             complement(3651..4793)
                     /locus_tag="VKQ52_14280"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLZ88418.1"
                     /translation="MNKILLSAFLLFFDCHPSSAQEQSKKNQPASSNPLITQSDKDID
                     GVVRQYLKSQKTVGLEIGILKDNKTLYYGYGETERGNRQLPDEHTIFEIGSITKTFTA
                     TLLAKAIVDGKMKLDDPVSKYLPDSLPVLEYRGMPATLQTIANHTSGIPGNPSNIKYP
                     PEIKGVWSGDPFKDYDDNDLFSFYAHFKLKRKPGEKFEYSNLAFGTLGVMLERIYGKS
                     FEQLVGEKISGPLGMVDTRQTVPPGDTPRVAHGYANNGQPAPAWHAKALAGCGALRST
                     ASDLLRYAQAEIGQGPLQLTAAIALTQQATYRQKKFGLGLAWVIIRLDRTMGVLHGGT
                     TGGFGSLLILIPNKKAALVILANNPLGVDDLAGDILSVMKKDEKLL"
     gene            complement(4924..>5257)
                     /locus_tag="VKQ52_14285"
     CDS             complement(4924..>5257)
                     /locus_tag="VKQ52_14285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ88419.1"
                     /translation="LGLFGLVTFAAEQRIKEIGIRKVLGAPITDIFTLLSKDFIRLLL
                     LSICIASPVAWWAMNKWLQDFAYRISIGWWMFVAVGAICLLIALITISFQVVKAAVAN
                     PVKSLRAQ"
BASE COUNT         1266 a         1308 c         1246 g         1437 t
ORIGIN      
        1 tgtctcgtcc taaaatagct atacaggtga aagaataaat cctgagaacg gctatacagg
       61 tgccttttta aggggatagt tgccgttcag gactaaggta ctggatttta tcttgtccgt
      121 acaccagcgg ccgtcccttg ctctgtgaag ggagcggtcc ggccgctggt gcggtctcta
      181 atcggggata ggatctccat ctttttttca tgggggtgag ggtttggtgg gcctggttgg
      241 gcgttaaatt gtcacagctg ccatggggcc tgatctcgtt gtaggccgtt atgctttcct
      301 gaaccttttg gcaggtttgt tcaaagttca gctggctggt atacaagtta aactcccctt
      361 ttatgatgcc attgatgcgt tcggcaagtg cattttcata cgggtcgcca ttctcggtca
      421 tgctgatggc tattccggca gcagtaagta catcgacata ctgtttgcaa caatattgag
      481 cccctctgtc ggaatggtgg atcaggggtt gttcttcgta ggaccgattg tccagggcca
      541 tttgcaaagc agtgacacat ccttcggcca gcatatcgct acgcaggcaa aagcccatga
      601 tcttacggga ccaggcatcg gtgatcaggc tcagatatcc ccattgattt tggatcctga
      661 tgtaggtcat atcgcttacc caaagctgtt cggggcgatg gatgaccaat tggccggtca
      721 ggttgctata ttttcgcatc cagtgccggg agtcggtggt ataggccttt cttttccgct
      781 gccggatcag cagcttgtgc tgtgccagca gatcaaataa ataatccctg cccacttcga
      841 taccatggct ggctaactcc ggggctatca gctcctgtaa ttttcgtgta cccacccggg
      901 gtagctgctg ccttgtcttt tgcacggctt gtaaaatgat ctcgtcggta aatatatcct
      961 gctgttctcg ccattgccgg tcataccagg catggcgggt tttgccaaac agtccgcaca
     1021 gcactttcat actgagggcg ggatagtcct ttttcagggt tttgactgct tggcaccagg
     1081 cttttttcga atgggaattt taaactgatc ctcggctaca tcaatgaggg tattgagcgc
     1141 cttgatcttc agctgcgctt cttccagcgc tttttgcagc gccttctttt cggcatcctc
     1201 ctggccgata gcagcattgg ttttaggctt catagccccg ctaagatcgc tattttcccc
     1261 gtcttgccgt aataagttga ggatcgcagt ataggacttc atattatagc tcagcttcgc
     1321 ttcctgaagg gtcattcgcc cttcccggat cgcccggatc atcgatcgct tttgggcatt
     1381 gttcagatgc ccctgcttac taatcttgta gcactcagat ccataattcc gcatccaatc
     1441 tgctatcgta ctgcgcgaca ccccatagat agcacatata tccctgcggc gcatccctcc
     1501 ctcgatggcc tcgacgatct tttgaaccgc taacttgtca taatgcccac ccgccttctt
     1561 gagcgccggc aactcatttt tcctgttttc caatgacata cactaagttt ttagtgtata
     1621 gcttttttag gacgggtcaa aatagggcgc gcgaaattgt ccttcaaaaa gaagaatagt
     1681 ttgctacctt aggaaaattt attttatgcc cgagcaatgg aataactttt ttgttatggt
     1741 tggtagcggc gcagcggcac tggcaggtct gattttcgtc gcgatgtcga tcaaccacga
     1801 gatcattatc cgaaacacga cacataagaa cagagcaatt aacatgctga caggcttcac
     1861 tgccattttc atggcaagca gttttgcgct tatcgggaat caagatccgg gagtgctcgg
     1921 ctttgagtgg cttgctctct ggttcgtcgc aacagttatc ttcatcaggg gttatgttat
     1981 agctatcagg tcgggtatga gctttatcgg attgaacacg ccgcgacttg caggcggtac
     2041 aatatgttac gtcgcggaag tcataggcgc aatttttctt attcgcggat atagctcagg
     2101 gctttacatc gcagctaccg caatcattgt tttattcgcc ttcctcattt caggagcctg
     2161 gctccttatg atcggcatct acgaagaccg ggctaagcga tgacctagac ggaacaccgg
     2221 ccatcgggga aagaaatatt tgcgcaaccg gaaagttgcg cgtatatttg caaccgatcg
     2281 gtttcccaaa ctaaaccgtg actattcatg agaagagata tttttcaggc cattgccgac
     2341 ccgacaagac gggcaatcat cgccttgatc gcattgcagg caatgacgcc aaacgccatt
     2401 gccgaaaatt tcaataccac acgacaagct gtttccaaac acctgcgcat cctgtcagaa
     2461 tgtgatctgg taaaacagga gtaccagggc agagaaattt actactcact tgaaattgaa
     2521 aaaatgaaag agatagacaa gtggctggac caattcagaa aaatttggga aacccggttc
     2581 acccagcttg acgaggtgct atcaacaatg aaaaaacagg aaaaatgagc ggagtgcaga
     2641 tcaagcgaaa aatcatcgtt accgaattca tcaccctcga cggggttatc gaggcgcccg
     2701 gcggcaatga aacccctcat cctcatggcg gctggcagtc taaatacagc tctcctgaag
     2761 ccgggaagta caaggttgaa gagcttgcca gcgtggatgc cttgctgttg ggcaagacca
     2821 cgtacgaagc gtttgccgcc tactggcctg gccagaccgg cgccgggttt gccgacccca
     2881 tcaacaagta tccgaaatat gtggtatccc gaagcctgca aaaggcggag tggaacaaca
     2941 gccatatcct tcgcgacgtg gccaaagacg tcgcggcgct gaagaagtcc gatggtggtg
     3001 atatcctcgt ctatgggagc gccaccctcg tcaaggccct gcttcaccac gacctcatcg
     3061 atgagttgcg gctgatggta tatcccctct cgatcggcgg cgggttgagg cttttcgatg
     3121 acaatcggga agcaaagaaa ttcgtgctca aacactcgcg cactaccgac aacggcgttc
     3181 ttatcctcga atatcagccg atcggttgaa ctatcgaaca actattttat actactcagt
     3241 atcagcaggc tcccaaagtt caaccttgtt gccttcaacg tcaataatat gaacaaactt
     3301 gccatagtca aaagcctcga ttttgtccac aattgttact ccttcgtttt taagttgctc
     3361 aacaagagct tctaaattct caactctata gtttatcata tagtctttcg tcgaaggatc
     3421 aaaatatttt gttgtttcag caaatggact ccatacagta gatccctttt ttgtcgggtc
     3481 tgcatcctcg cgccactcaa aattcgcccc atattggttt gtgtctaagc ctaaatgcgt
     3541 tttgtaccat tctttcattt tattggggtc tttacattta aaaaagatgc cgccaattcc
     3601 tgttaccttt ttcattatta ccgtttttga atcactaata tagtataaac ctacaataac
     3661 ttttcgtctt tcttcattac tgaaaggata tctcctgcca ggtcatctac tccgagcggg
     3721 ttgttggcga ggatcaccaa ggcggctttt ttgtttggga taagtatcaa caaactcccg
     3781 aaacctcccg tagttccgcc atggagtacc cccatggtcc tgtccagcct gataataacc
     3841 cacgcgagtc cgagtccgaa ttttttttgt cgataggttg cctgctgggt aagcgcgatg
     3901 gcagccgtta gttgaagagg cccctgccct atttccgcct gtgcatatcg tagaagatcg
     3961 gacgccgtcg aacggagcgc gccgcaccca gctaatgctt tagcatgcca cgccggggcc
     4021 ggttgtccgt tgttggcata tccatgggca acccgcggag tgtcaccggg aggcaccgtt
     4081 tgcctggtgt ccaccatgcc cagcggccca ctgatctttt cccctaccaa ttgttcaaag
     4141 gacttcccat atatcctttc aagcattacg cccagggtgc caaaggcaag atttgaatat
     4201 tcgaattttt cgcccggttt gcgtttgagt ttaaaatgag catagaaact aaacaggtca
     4261 ttgtcgtcat agtctttaaa aggatctccg gaccaaacac ctttgatctc aggggggtat
     4321 tttatgttgg agggattccc cggaataccc gacgtatggt tggcgattgt ttgtagtgta
     4381 gccggcatcc cgcgatattc gagaaccggc agggaatccg gcagatattt actcaccgga
     4441 tcatccaatt tcattttgcc atccactatt gccttcgcca gcaacgtggc cgtgaacgtc
     4501 tttgtaatgg atccaatctc aaagatggta tgctcgtctg gcagttgccg gttgccccgt
     4561 tcggtctcgc cgtagccata ataaagggtc ttattatcct tcagaatgcc aatttccagg
     4621 ccgaccgtct tttgcgattt caggtactgc cggactaccc catcgatatc cttatcggat
     4681 tgggtgatca aaggattgga agacgccggt tgattttttt tggattgctc ttgcgcggag
     4741 gaggggtggc agtcgaaaaa aagtaaaaag gcggatagca gtatcttatt cataagaaaa
     4801 ggcgctgagg ttaaaggacc ggctaaaaat aacccgtcat ttattgattc gccagggaca
     4861 tggggaattt taacaggatt atagcgaaat catcattggc ttgtattgcg cgcaatgcgc
     4921 gtattattgt gctctcaagc tcttcaccgg attggcaact gccgccttga ctacctggaa
     4981 acttatagta ataagtgcta tcagcaaaca aatagcgccg actgccacaa acatccacca
     5041 gccgatactg attcgatagg caaaatcctg tagccatttg ttcatcgccc accatgcaac
     5101 aggtgacgca atacaaatgg acaataaaag tagtcttata aaatctttgg acagcaatgt
     5161 aaagatatcc gtgatcgggg ctcccaaaac ctttcgaatg ccgatctctt ttatacgctg
     5221 ttctgccgcg aatgtgacaa ggccgaatag gcccagg
//
LOCUS       DATGFG010000760         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1031641, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000760 DATGFG010000000
VERSION     DATGFG010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031641"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2340)
                     /locus_tag="VKQ52_14290"
     CDS             complement(<1..2340)
                     /locus_tag="VKQ52_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88420.1"
                     /translation="MAKFVDKQIIITGLLLTLGSAALAQAEKDSLQASFARFQSSIPQ
                     EKLFVHVDKTVYLAGETIWFKVYAVDARSNTPDSLSAIAYVEIIDRDQKPVLQEKVAM
                     PDGKGDGYFRLPPSIPSGHYILRAYTNWMKNFSPDLFYQQEVTILNTLTETGFEDSSR
                     HAADSIRFFPEGGNLVNGLTSVIAFKAVNPAGEGIACRGVVIDEHKDTVARFQSNHVG
                     MGSFSLTPVKGNTYHAQIATPSSTITEKLPPAFDQGYVMHLDPPEGKKLRITVNTATN
                     PASPVVYLLVHTRGRLKSIQLNYLNNKKTGFSVDTDSLDEGISHFTILNGERLPLCER
                     LWCKPPRQQLHIGVNSPAPAAGYGPREKITVHLTTTDPSGHPIPANLSMSVFLLDSLQ
                     SIPAENILSYLLLRSDLKGNIESPGYYFAGNDPATTEALDDLMLTQGWSRFRWEDLRR
                     NKRPYFEFLPETNGFIIGGRMIDKRTSLPPSPAIGYLSVPGKNFEFATAIGHPDGSLN
                     FHLGSFYGNREIIVQTDSRTDSNYRIDIAGSFSDRYSWQPTPGPLFPAGAQEQLLFRS
                     INCQAENAYRAAEKHHLLPLPPEDTIGFYGHADLRYNLDDYTRFVTMDEVIREFVQDV
                     RVRRSSDHAHFRVRNALFNTFFEDDPLLLVDGVPVFDGDNMVGINPLKIEKIDVVSHR
                     YHFGPSFTDGIVSFLSYDGDLGGYRLDPSAVVIPFAGLQQHQEFYSPIHTPGVQSPLP
                     DLRNQLLWSPEIDTDAAGKKQLSLYTSDLKGKFALVVQGM"
     gene            complement(2352..>2752)
                     /locus_tag="VKQ52_14295"
     CDS             complement(2352..>2752)
                     /locus_tag="VKQ52_14295"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HLZ88421.1"
                     /translation="EDAYNYWLLIQKTSDNLGTLFDLQPTQLVGNIHCLTNPSEPVIG
                     FLSATSVQQQRIFIYRYYLGTWPHNEPGYGCDTIKIPVNPVNPLIYNYPDTLYAPWYF
                     ISNGPLVLGSRFCLDCTLFGGTNSKPSFWQ"
BASE COUNT          555 a          622 c          948 g          627 t
ORIGIN      
        1 catgccctgt actaccaggg cgaatttccc ttttagatcg gaggtgtaca gcgagagctg
       61 ttttttcccg gccgcgtccg tgtcgatctc gggtgaccag aggagctggt tgcggaggtc
      121 gggcaggggg ctttggacgc cgggtgtatg gatgggggaa tagaattcct ggtgctgctg
      181 gagtccggca aaggggatga ccaccgcgga ggggtccagc cggtatccac ccaggtcgcc
      241 gtcgtaagac aggaagctca cgatgccatc ggtgaaggac gggccgaaat gatagcgatg
      301 ggagacgacg tctattttct cgatcttcag cggattgatc ccgaccatat tgtcgccgtc
      361 gaacacgggg accccatcga cgagcaggag ggggtcgtcc tcgaagaagg tgttgaagag
      421 ggcgtttcgt acgcggaaat gggcgtggtc cgagcttcgc cgtacgcgaa cgtcctggac
      481 aaattcccgg atgacctcgt ccattgtaac gaaccgggta tagtcgtcaa gattataccg
      541 caggtcggca tggccgtaaa aaccgatggt gtcttcgggt ggaaggggga ggaggtgatg
      601 tttctccgcg gcccgatagg cgttttccgc ctggcagttt atgctgcgaa agaggagttg
      661 ctcctgcgct cccgcaggga agagcgggcc gggggtgggt tgccaggaat agcggtcgga
      721 aaaggagccg gcgatatcga tgcgatagtt gctgtcggtt cggctgtcgg tctgtacgat
      781 gatctcccgg ttgccataga agctgccgag atgaaagttg aggctaccat cgggatggcc
      841 aatggcggtg gcgaattcaa aatttttacc tggtaccgag agatagccga tggcgggaga
      901 ggggggaagg cttgtccgtt tatctatcat tcttcccccg atgatgaacc cgtttgtctc
      961 cggcaggaat tcaaaatagg gtcgtttatt ccgacggaga tcttcccagc ggaagcggga
     1021 ccatccctgg gtgagcatca ggtcatccag cgcttccgtc gttgccgggt cgttgccggc
     1081 gaagtagtat ccgggagatt ctatattccc tttcaggtcg gagcgcagga gcaggtagct
     1141 gaggatattc tccgccggga tggactgcag ggagtcgagg aggaataccg acatggacag
     1201 gttggcgggt atggggtgac cggaggggtc tgtcgtggtg aggtggacgg ttatcttttc
     1261 gcgtgggccg tagccggcgg cgggggccgg gctgttcacg ccgatgtgta attgctgccg
     1321 gggcggcttg caccagagcc gttcgcagag gggcagtctt tcgccgttga ggatggtaaa
     1381 gtgggagatc ccttcgtcga ggctgtccgt atcgaccgag aagccggtct ttttgttgtt
     1441 gagatagttc agctggatgg acttcagcct gccgcgggta tgcacgagga ggtaaaccac
     1501 cgggcttgca gggttggtgg ccgtgttgac ggtaatacgg agtttttttc cttcgggtgg
     1561 atcgagatgc atgacataac cctgatcgaa tgccgggggg agtttttccg ttatggtgga
     1621 agagggtgtc gcgatctgtg catggtaggt attccctttg acgggtgtca gtgagaacga
     1681 tcccatacca acgtgattgg attggaaacg ggcaaccgta tccttatgtt cgtcgatgac
     1741 cacgccccgg caggcgatcc cttcgcccgc ggggttgact gccttgaatg cgataacgga
     1801 ggtgagaccg ttgacgaggt tacccccttc cggaaagaac cggatactat cggccgcatg
     1861 gcgggaagaa tcctcgaagc cggtttcggt caacgtgttc aggatcgtca cctcttgctg
     1921 gtaaaagaga tcggggctga aattcttcat ccagttggta taggcgcgaa ggatataatg
     1981 accggagggg atggaggggg gtagccggaa atagccgtcg cctttgccgt ccggcatggc
     2041 cactttttcc tggaggacgg gcttctgatc gcgatcgatg atctcgacgt aggcgatggc
     2101 ggaaagggaa tcgggagtat tggatcgggc gtccacggcg tagaccttaa accagatggt
     2161 ctcgccggcg aggtaaaccg ttttgtccac atgtacgaag agtttttcct gcgggatcga
     2221 ggactggaaa cgggcgaagg aggcttgcag gctatccttt tccgcctggg caagggcggc
     2281 gctgccgagc gtgagcaata ggcctgtgat gatgatctgt ttatcgacga atttggccat
     2341 cgtactgtgt tttattgcca gaaggagggt ttgctattgg taccgccgaa aagggtgcag
     2401 tcgagacaga agcgggagcc gagcaccagg ggaccgttgc tgatgaaata ccagggggca
     2461 tatagggtat ccggatagtt gtagatgagc gggttgacgg ggttgaccgg tatttttatc
     2521 gtgtcacatc catagcccgg ctcgttatgg ggccaggtgc ccaggtagta ccggtagatg
     2581 aatattcgtt gctgttgtac ggaggttgcg gagaggaagc cgataacggg ttccgaagga
     2641 ttggtgaggc agtggatatt gccgaccagt tgtgtcggct gaagatcgaa aagggtgccc
     2701 aggttatcgg aggttttctg gatcagcagc caataattgt acgcgtcctc cg
//
LOCUS       DATGFG010000761         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1557409, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000761 DATGFG010000000
VERSION     DATGFG010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557409"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VKQ52_14300"
     CDS             <1..1173
                     /locus_tag="VKQ52_14300"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HLZ88422.1"
                     /translation="TYNEEKVAQGQAVLIHLNNFLQDKNNITYDDKLERFQRLNELNT
                     RSRVNMLHATLNFHPSEKLSDEQLSSIADRYMQGMQMEDQPYLVYRHQDARHPHIHLV
                     TSLIRPDGTRVNTHRMAIRLSEPTRKAIETEFQLLPARRPEASVPLDPNEVRKITPGD
                     DTPLSQSVDRILTSVNRHYHFTNLNEYNAILRAYNVTAETGNPGSKTRRYDGIYYVAL
                     DDLGNKISPPMMASQLPSRPTLHRLHEKFHQPAANHLDHLASVRQRIDWALDQRPPTL
                     RHLVSQLQGDGIEIVIPPGNGRNPHDQIFVDHRTRTAVTGATLGPGYTSAAINNSIDH
                     GKKAGQRQHQTAQLPEGTHFNASVPQVLSAVLRTDPGGQRPDAIGQDQHLGPRRKL"
     gene            1198..>2640
                     /locus_tag="VKQ52_14305"
     CDS             1198..>2640
                     /locus_tag="VKQ52_14305"
                     /inference="COORDINATES: protein motif:HMM:NF025651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YWFCY domain-containing protein"
                     /protein_id="HLZ88423.1"
                     /translation="MQPTNDNQFRELRNLAAACCAALIVLHYYYFCHLGLAAWSFTHP
                     IPDRLVAGFASKGLFRHPALSLALALLALAATTIGAKAPPQVRPRRLITIHLSGILLY
                     FGGFFILYLDADPKVLTVSYITITLTGLGLLYTGIRGLAGLLTWPGKRAVFNQYNESF
                     PQEERTLTHANFFHLRGRYRFQNKWRENVVNLDIYRGTIIMGSPGSGKTRYIFKQLIR
                     QSIEHGMAVFVFDLKYPGLSRFVYNELERVRPKLNKSLQFYSFNFNNLDHRCNVLAPE
                     TMEDLSDAIECARTLFYGINRRASKLQGEFFVESAVDFFAACIWFLRQYESGRYCTLP
                     HLIELMAIDYARLFSVLRSYPDLATLVQPFTDALQYNVMDQLQGQLDSARIPLFSLVS
                     PKLYYLLSASDFSLAINDPKAPKIVCLGSDPQKQHIYGAVISLFVSRMLKMINQPGGT
                     PCQVYIDEFPTFHSLSMDTTLATCREHKVGI"
BASE COUNT          591 a          930 c          573 g          546 t
ORIGIN      
        1 acttacaacg aggaaaaggt ggcacaggga caggccgtac tcattcacct caataatttt
       61 ttgcaggaca aaaacaacat cacctacgac gataagctcg aacggttcca gcgactcaac
      121 gaactcaaca cgcggtcacg cgtcaatatg ttgcacgcca cgcttaactt tcacccttct
      181 gaaaagctct cagacgaaca gttatcctcc atcgccgacc ggtacatgca aggcatgcaa
      241 atggaagatc agccctatct cgtctatcgc caccaggatg cccggcatcc ccacatacat
      301 ctcgtcacct ccctcatccg gcccgatggc acacgcgtca atacccatcg catggccatc
      361 cgcctgtccg aaccaacccg taaagccatc gaaaccgaat tccaactgct tcccgcccgg
      421 cgaccggagg cgtccgtccc acttgacccg aacgaggtcc gcaagatcac tcccggcgat
      481 gacacacctc tctctcaatc tgtcgatcgc atcctcacca gcgtgaaccg gcactatcat
      541 tttaccaatc ttaatgagta taacgccatc ctccgtgcct acaatgtcac cgccgagacc
      601 ggcaatccag gcagcaagac ccgccggtat gatgggattt attacgtcgc cctcgatgac
      661 ctgggcaata agatcagccc gcccatgatg gcctcccaac tgcccagccg gccgacactt
      721 caccgtcttc atgaaaaatt ccatcagccc gccgccaacc acctcgacca tctggcttcc
      781 gtccgccagc gcattgactg ggccctggac cagcggccgc caaccctgcg ccacctggtc
      841 tcccaactgc agggagacgg catcgagata gtcataccac ccggcaacgg caggaatccg
      901 cacgaccaaa ttttcgtgga tcaccgtact cgaacggccg tcaccggcgc aacactcggc
      961 cccgggtata cgagcgcggc cattaacaat tccattgatc acggcaagaa ggcaggccaa
     1021 cgacaacacc agaccgctca gttgccggag ggtacccact tcaatgccag cgtaccccag
     1081 gtcctctccg cggtccttcg caccgacccc ggcggccagc gccccgatgc catcgggcag
     1141 gaccaacacc ttggcccccg ccgcaaacta tagtttatca caatcaaatt agttattatg
     1201 caacctacaa acgataatca attccgcgag cttcgcaatc tcgcagcggc ctgctgtgca
     1261 gcgctgatcg tccttcacta ctattatttc tgccacctgg gtctcgcggc ctggtctttc
     1321 acccacccta tccccgaccg ccttgtggcc ggcttcgcca gtaaaggcct gttccgtcac
     1381 ccggctcttt ccctggccct ggcgctgctc gcccttgctg ctaccaccat cggagccaag
     1441 gcgcctcccc aggtcaggcc ccgccgcctg atcaccatcc acctcagcgg catcctgctc
     1501 tacttcggcg gcttcttcat cctctatctc gacgccgatc cgaaggtcct caccgttagc
     1561 tacataacga ttaccttgac agggcttggc ctgctctata ccggcattag aggactcgcc
     1621 ggcctgctta cctggccggg gaaacgcgcc gttttcaacc aatacaatga aagttttcca
     1681 caggaagaac ggactttgac ccacgctaat ttcttccatc tgcgcggccg ttaccgcttc
     1741 cagaacaaat ggcgcgaaaa cgtcgtcaac ctggatattt ataggggaac gatcatcatg
     1801 ggcagccccg gatcaggtaa aacccggtac attttcaagc aactcatccg gcaatccatc
     1861 gaacacggca tggccgtttt cgtcttcgac ctaaagtacc ccggcctcag ccggttcgtc
     1921 tacaatgagc tcgaaagagt acggcccaag ctaaataagt cgctgcaatt ctattctttt
     1981 aacttcaaca acctcgatca ccgctgcaat gtccttgccc ccgagaccat ggaagacctg
     2041 tccgacgcca tcgaatgcgc ccgaacactt ttctatggca ttaaccgccg ggcctccaaa
     2101 cttcagggcg aattcttcgt cgagtccgca gtcgacttct tcgccgcctg tatttggttc
     2161 ctccgtcagt atgagagcgg tcggtactgt accctccccc acctgatcga gcttatggcc
     2221 atcgactacg cccgcctctt cagcgtgctc aggtcctatc ccgacctcgc cacgctcgtc
     2281 cagcccttca cggatgccct ccaatacaat gtcatggacc aactgcaggg acaactcgac
     2341 agcgcccgca ttcccctgtt ttccctggta tccccgaagc tttattatct gctatcggcc
     2401 agcgatttca gcctcgctat caacgacccc aaggcaccca agatcgtctg tcttggttcc
     2461 gacccgcaaa agcagcacat ctatggcgct gttatcagcc tcttcgtcag ccgcatgctc
     2521 aaaatgatta accagcccgg gggcaccccc tgccaggtct acattgacga gtttcccacg
     2581 tttcactccc tgagcatgga caccaccctg gccacctgcc gggagcacaa ggtcggtatt
//
LOCUS       DATGFG010000762         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1165991, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000762 DATGFG010000000
VERSION     DATGFG010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165991"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..652
                     /locus_tag="VKQ52_14310"
     CDS             257..652
                     /locus_tag="VKQ52_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88424.1"
                     /translation="MKSVYPVIFTLITLNIHALPAKPSVRLSAQTLAQPTTHPTTAQP
                     STHPATQPSPQPTAQPSTQLTTQTSPQPQTSPQSTDQPSTQPSSPSLAARYPIIPWPT
                     SLTPQDGDFLITSATTLKADTVFATKPRP"
     gene            667..2799
                     /locus_tag="VKQ52_14315"
     CDS             667..2799
                     /locus_tag="VKQ52_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HLZ88425.1"
                     /translation="MRTTSAQPAIEMKQDSAITAEEGYTLTITKKSILITARTPAGMF
                     RAVQTIRQLLPPAAETAVTAPTPKTTTNLAAGTPANLTAGTSQHPARGWKLPAVTISD
                     APAYAWRGMHLDVSRHFFSIAYLRKFIDIMALYKMNKFHLHLTDDQGWRIEIKKYPLL
                     TTVGAWRTFNKQDSACMRRAKDDPDFTIDPAHIIQKDGKTLYGGFYTQDEMRALVAYA
                     AERHIDIIPELDMPGHMMAAIDQYNWLSCDSTSAFGKLFSTPICPCLPATYQFAEDVW
                     SEIMDIFPSTYLHIGGDEVDRTLWAKSADCKQLMEKEGLKTTAQLQSYFIDKMERFFN
                     SKGRKLIGWDEILEGGISSTALVMYWRTWVPKAPIEAAQHGNSVIMTPGNPLYFDYTQ
                     DRNSLPNVYNFNPIPHGLTPDQAKNIIGAQANIWTENIPTENRADYMFMPRMTALAEV
                     LWTGRPANYDFFLQRLSRAYPRLDKLHVRYRLPDLTGFLQSNVFVDADTLTIKKPLDN
                     MAIRYTTDGALPDAHSPALNGPLIIRDNKHLRIAAFRPDGSRGDSYDLTYQKQSLAEP
                     EFLPSTIAGLTCSRFKGSFKTTAEMTGRQPDKTVDVYQFTVPSTLEAPAFGLQYRGYL
                     DIPEDGVYSFYLTCDDGGILTIAGREVVNNDGNHAPLEKDGQVALKRGPQKIALDFIE
                     GGGGYTLRLQYSFNGSAPRDIPTAWLKH"
     gene            2830..>3279
                     /locus_tag="VKQ52_14320"
     CDS             2830..>3279
                     /locus_tag="VKQ52_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLZ88426.1"
                     /translation="MTRKHIFSLPRLFSAAILCATLLLPALALLAPLTLLAQAPPKPY
                     GALPTPRQLAWQETEMYCLIHFGVDTYTDKEWGYGDEDPAILNPIHFDAKQIVGAAKA
                     GGFKGVVIVAKHHDGLCLWPTKTTEHNITKSPWRNGHGDMIREYHSAC"
BASE COUNT          824 a         1234 c          665 g          556 t
ORIGIN      
        1 cttcggccac gatgtcctcc gccacctcct tacgatgggt atacatcatc gagaactgga
       61 tcagcgaaga atttagcagg tggaacagcg actcgaacgc ccgttgatca tcctctccgc
      121 tgatccgctg ccaaagctgc agaatatcaa ttttcttttt tttcaaagca gttttaaccc
      181 ttctgattgc tctaaactac aaatcttttt cttcctttgt aggcaattcg acttaaaaaa
      241 ttgtccaaac gaatatatga agagcgtata ccccgtcatt tttacactaa taaccctcaa
      301 catccacgcc ctccccgcca agccatcggt acgactatcc gctcaaacat tggcccagcc
      361 cacgacgcat ccaacaactg cccaaccctc gacgcatcca gcaacccaac cctcgcccca
      421 accaactgcc caaccctcca cacaactaac aacccaaacc tcgccccaac cacaaacctc
      481 accccaatca acggaccaac cctccacgca accatcgtcc ccctccctgg ctgcgcgcta
      541 tcccataatc ccctggccca cctccctgac cccgcaggac ggcgacttcc tcatcacatc
      601 cgccacaacc ctaaaggcag acaccgtctt cgcaacgaag cccaggccct gaaaaccctc
      661 ctcggcatcc gcacaacgag cgcccagccc gccatcgaaa tgaaacagga ctccgccatc
      721 actgccgaag aaggatacac cctcacgatc acaaaaaaaa gcatcctcat cacggcccgt
      781 acacccgccg gcatgttccg cgccgttcaa accatccgcc agctactgcc tcccgccgcc
      841 gaaacagccg tgacagcccc aacgcctaaa accacaacaa acctcgctgc cggcaccccg
      901 gcaaacctca cagccggcac cagccaacat cctgcccgcg gctggaaact ccccgccgtc
      961 accatcagcg acgcccccgc ctacgcctgg cgcggcatgc acctcgacgt ctcccgccac
     1021 ttcttctcca tcgcctacct ccggaaattc atcgacatca tggccctgta caaaatgaac
     1081 aaattccacc tccacctcac cgacgaccag ggctggcgca tcgaaataaa aaaatatccc
     1141 ttactgacga ccgtcggcgc ctggcgcacc ttcaacaaac aggactcggc ctgcatgcgc
     1201 cgcgcaaaag acgaccccga cttcaccatc gaccccgcac acatcatcca aaaggacggc
     1261 aaaaccctct acggcggttt ctatacgcag gacgagatgc gcgcactggt ggcctacgcc
     1321 gccgaacgcc atatcgacat catccccgag ctagacatgc ccggacacat gatggcggcc
     1381 atcgatcaat acaactggct cagctgcgac agcaccagcg ccttcggaaa actattctcc
     1441 acgcctatat gcccctgcct cccggctacc taccaattcg cggaggacgt ctggtccgag
     1501 atcatggaca tttttccctc cacttatttg cacattgggg gtgacgaggt cgatcgcacc
     1561 ctctgggcaa agtcggcgga ctgcaaacaa ctgatggaaa aagaaggact gaagaccacg
     1621 gcccaactcc aaagctactt catcgacaaa atggagcgct tcttcaactc gaaaggccgg
     1681 aaactcatcg gctgggacga gatcctcgaa ggcggtatca gcagcacggc gctggtcatg
     1741 tactggcgca cttgggtgcc aaaagctccc atcgaggcgg cacagcacgg caattccgtc
     1801 atcatgaccc cgggcaaccc cctctatttc gactacaccc aggaccgcaa ctccctgccc
     1861 aacgtatata actttaatcc catcccccat ggcctcacac ccgatcaggc aaagaacatc
     1921 atcggcgccc aagccaacat ctggaccgaa aacatcccca ccgagaaccg cgccgactat
     1981 atgttcatgc cgcggatgac cgccctcgcg gaagtcctgt ggacaggccg ccccgccaac
     2041 tatgacttct ttctccaacg gctgagccgc gcctatcccc gtctcgacaa actccatgtc
     2101 cgctaccggc tccccgatct caccggcttc ctgcaatcaa acgtcttcgt cgacgcggac
     2161 accctcacga taaaaaagcc cctcgacaat atggccatcc gctataccac cgacggcgcc
     2221 ctcccggatg cccactctcc ggcgctgaac ggcccgctca tcatccgcga caacaaacac
     2281 ctgcgcatcg ccgccttccg ccccgatggc tcccgcgggg atagctatga tctcacatac
     2341 caaaaacagt ccctggccga acccgaattc ctcccatcca cgatcgccgg cctcacctgc
     2401 agccgcttca aaggctcctt caaaacgacg gcggaaatga ccggcagaca acccgataaa
     2461 accgtcgatg tatatcagtt caccgttccc tccaccctcg aggcccctgc cttcggattg
     2521 caataccgtg gctatctcga cattcccgag gatggcgtct atagcttcta cctcacctgc
     2581 gacgacggcg gcatcctcac catcgccggc cgggaagtcg ttaacaacga cggcaaccac
     2641 gccccattgg aaaaggacgg ccaggtggcg ctgaaaagag gcccgcaaaa gatcgccctc
     2701 gacttcatcg aaggcggcgg cggctacaca ctccggttgc aatacagctt caacggctcc
     2761 gcgccgcggg atattcccac ggcatggcta aaacactaat cacaataaca gcacaacaac
     2821 agcacaataa tgacacgcaa acacattttc tcccttccgc gcctcttctc cgccgccatc
     2881 ctctgcgcaa ccctcctcct cccggccctg gcgctcctcg ccccactcac cctcctcgcc
     2941 caggcgcccc caaaacccta cggcgccctc cccacaccgc ggcaactcgc ctggcaggaa
     3001 acagaaatgt actgcctcat ccacttcggc gtcgacacct acaccgacaa ggaatggggc
     3061 tatggcgatg aagaccctgc catcctcaac cccatccatt tcgacgcaaa acagatcgtc
     3121 ggggcggcaa aagccggcgg cttcaaaggc gtcgtcatcg tcgccaaaca ccacgacgga
     3181 ctctgcctct ggccgacaaa aacaaccgaa cacaacatca caaaaagccc ctggcggaat
     3241 ggccacggcg atatgataag ggaataccac tctgcctgt
//
LOCUS       DATGFG010000763         3620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1631047, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000763 DATGFG010000000
VERSION     DATGFG010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631047"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VKQ52_14325"
     CDS             complement(<1..1122)
                     /locus_tag="VKQ52_14325"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HLZ88427.1"
                     /translation="MRYRTKLFATLFTGLLSGSMLVQAQEAQPPADEAWKKVYRATAT
                     RYDDLIHTKLEVKFDYDKSYMYGKAWVTLKPHFYPTDTVALDAKGMDIHSVAIEREGK
                     TSPLKYDYDGMVLRVHLDKVYRYTEKYTLFIDYTAKPNELKVKGSAAITDAKGLYFIN
                     PKGEDKNKPTQIWTQGETEANSVWFPTIDKPNQKTTEEIYMTVPSKYVTLSNGLMVGQ
                     KENGDGTRTDHWKMDLPHASYLFFMGVGDYAIVKDSYKGKDVNYYVEKQYAPVARRIF
                     GNTPEMIAFYSRITGIDYAWPKYDQIVGRDYVSGAMENTTCTLHGEAAQQDARELTDG
                     NGWEDVIAHELFHQWFGDLVTTESWSNITLNESFADYSET"
     gene            1434..3185
                     /locus_tag="VKQ52_14330"
     CDS             1434..3185
                     /locus_tag="VKQ52_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88428.1"
                     /translation="MCKLGLVLLFSFLVGHSYAQDYFVLLQSDNRQPFYVRMGGQLYT
                     SSPDGHLILSQLKDSTYTINVGFPGHAGYRPTGQSGTSQASPGSPADLEQIYTFTTAQ
                     KDREFQIKEHGGAGWGLYDLQTNDWLAVQGRSGGREEVRAIGIRRDDAFSRMMAGVVQ
                     DTAVLYNDYANPNLAADSPANPALASTLPANTTPATRATTTVPGATAPTTTATITSPG
                     ATASQDSIIATNIPLINKPLTDTSATVATTADTANTRLFRPRPDSAVATNTKADTPAG
                     RSVADSPNTAMAKPASDTPAIVKSAAPITKSTPDTPATHTTATTADSATASAPLYRPV
                     AGTASHRSAPVVDSASGHPLYRPLPEVVKLSEKKGTRNMRLVYADKGRNARPDTVVVI
                     IPLDTPQTATAKPRGPNPDSSRLAATRARAQNPDSSRSSSGRGHDPNSDTPTVGQGST
                     AVHLIIPTPVNPPADKTRGVDSPKKTTKTALPYVNSDCHNFATDYDVDKLRVKMLEST
                     RDEERVAAALKVFKTKCFFTRQVRALSEVFTTDAAKYRFFETAYPFAADEHFRDLGTL
                     LSDPVYAGKFKTLTGTH"
     gene            3215..>3620
                     /locus_tag="VKQ52_14335"
     CDS             3215..>3620
                     /locus_tag="VKQ52_14335"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HLZ88429.1"
                     /translation="MPRYFLELAYKGTNYSGFQIQENASSIQQEVEKALAIFFRSNFE
                     LTGSSRTDGGVHALQNYFHFDYEGIIENRVLYNINAILPPDIVLTGIHPVAPDAHCRF
                     DAISREYRYYIYRVKNPFLADRAWFYPYTLDRQ"
BASE COUNT          764 a         1268 c          852 g          736 t
ORIGIN      
        1 ggtttcgcta tagtcggcga aggactcgtt cagggtgata ttgctccagc tttcggtcgt
       61 gacaaggtcg ccaaaccact ggtggaacag ctcgtgtgcg atcacatcct cccagccgtt
      121 gccgtcggtc agttcccgcg catcctgctg cgccgcttcc ccgtgcaggg tgcaggtggt
      181 attctccatc gccccgctca catagtcccg gccaacgatc tggtcatact tgggccaggc
      241 atagtcgatc cccgtgatcc gcgaatagaa cgcgatcatc tccggcgtgt tgccgaaaat
      301 gcgcctggcc accggggcat attgtttctc tacataataa ttgacgtcct tgcccttata
      361 ggagtctttt acgatcgcgt aatcacccac ccccataaag aaaagatagg atgcatgtgg
      421 cagatccatt ttccagtggt cggtgcgggt gccgtcgcca ttctccttct gacctaccat
      481 caacccattg gacagggtca cgtatttgga cggaacagtc atatagatct cttccgtcgt
      541 cttctggtta ggcttatcga tcgtcgggaa ccacaccgaa ttagcctccg tctctccctg
      601 cgtccagatc tgcgtcggct tattcttgtc ctcgcccttg gggttgataa aatacagccc
      661 cttcgcatcc gtgatcgctg cgctcccctt taccttcagc tcgttcggct tcgccgtata
      721 gtcgatgaaa agcgtatact tctccgtgta tctgtacacc ttgtcgaggt gaacccgcaa
      781 caccatcccg tcataatcgt acttcagtgg gctggtcttc ccttcccttt cgatcgccac
      841 cgagtggata tccatcccct tggcatccag cgccaccgta tcggtaggat agaagtgggg
      901 cttcagggtc acccaggcct taccgtacat ataggacttg tcgtagtcga acttgacctc
      961 caatttcgtg tggatcaggt cgtcatacct ggtggccgtc gcgcggtaca cctttttcca
     1021 ggcttcatcc gccgggggct gcgcttcctg ggcctgaacc agcatgctgc cggacaggag
     1081 tcccgtgaac agagtggcaa acaattttgt tcgatacctc atgtgttgat aaacgtgcat
     1141 agtgattttt ttcttggcga taaagataat cgttgatggg agacctttct ccgccgtcta
     1201 tccctttttc ccgcccctcc gtccgctcac cggcgcctcc ccgcccgctc ctccactcgc
     1261 ccccgccctg ctgtccccgg atagtatggc aattaatcga taaatgtatc gttcatgcaa
     1321 tctgcgggct agcggttttc cttaacttgc agtaggttta tcaccattaa ccctattaaa
     1381 attggcgagg cgcctgtctg ccgcgggttt gccggagcca aataccccaa atcatgtgta
     1441 agctcggtct ggtattattg ttctcattcc tggtgggtca cagctatgca caggactatt
     1501 ttgtcctgct ccaatccgac aatcggcaac ccttctatgt ccgcatgggc gggcaactat
     1561 atacatcctc tcccgacgga caccttattt tatcgcaatt aaaagacagt acgtatacga
     1621 taaacgtagg cttccccggg catgccggct atcgtcccac gggccagtcc ggcacaagcc
     1681 aggccagccc cggcagtcct gccgacctgg agcagatcta cacctttacc accgcgcaga
     1741 aggacaggga attccagata aaggagcacg gcggggcagg gtgggggctc tatgatctgc
     1801 agacaaacga ctggctcgcc gttcagggcc ggtcgggcgg gcgggaagag gtacgggcca
     1861 tcggcattcg cagggatgac gccttctccc gcatgatggc cggcgtcgtc caggacaccg
     1921 cggtactata taatgactac gccaacccca acctggccgc cgacagcccg gcgaatcccg
     1981 cccttgcgag caccctgccg gcaaatacca ccccggcgac cagggcgaca accaccgttc
     2041 ccggagccac cgccccaacc accactgcga caatcacatc ccccggagcc accgcaagtc
     2101 aagactcgat catcgcgacc aatatccccc tgatcaacaa acctctaacg gatacctccg
     2161 ccacggtcgc aacaacggcc gacaccgcca atacccgctt attccggccg cgccctgaca
     2221 gtgccgtcgc caccaatacc aaggccgata ccccggccgg caggtcagtg gccgacagcc
     2281 ccaacactgc catggccaaa cccgcctcgg acactcccgc catcgtcaaa tccgccgccc
     2341 cgataaccaa atcgacccct gacaccccgg ccacccatac caccgcgaca acagccgaca
     2401 gtgccaccgc ttctgccccc ctctaccgcc ccgtggccgg cacggcctcg cacagatccg
     2461 ccccggtggt cgactccgcc tccgggcatc ccctctatcg gccgttaccc gaggtcgtca
     2521 aactcagtga aaagaaaggc acccgcaata tgcgcctggt ctatgccgac aaaggcagga
     2581 acgccaggcc cgatacggtg gtcgtgatca tcccgctcga caccccgcaa accgcgaccg
     2641 ccaaaccccg tggtccgaat cccgacagca gccggctggc cgccaccagg gcccgtgcgc
     2701 agaacccgga ctccagccgg tcatccagcg gccgcggaca tgatcccaac tccgataccc
     2761 cgaccgtcgg ccagggcagc acggccgtcc acctgatcat cccgaccccg gtcaatcccc
     2821 ccgccgacaa aacccgtggg gtcgattcac ccaaaaagac gacaaagacc gcactcccct
     2881 atgtcaactc cgactgtcac aacttcgcca ccgactatga tgtcgacaag ctgcgcgtaa
     2941 aaatgctcga atccaccagg gacgaggagc gcgtcgctgc cgccctcaag gtctttaaga
     3001 caaaatgctt cttcacccgc caggtccgcg ccctcagcga ggtcttcacc accgacgccg
     3061 ccaagtaccg gttcttcgaa accgcctacc ccttcgccgc cgacgaacac ttccgcgacc
     3121 tcggcaccct cctgtccgac cccgtctatg ccggcaaatt caaaaccctc accggcaccc
     3181 actaacgagc ccgccccacc ttatctttgc gcctatgccc aggtacttcc tagaactcgc
     3241 ctacaaaggc accaactaca gcggattcca gatccaggag aacgcctcct ccatccagca
     3301 ggaggtcgaa aaggccctcg ccatcttctt tcgaagcaat ttcgaactca ccggctcctc
     3361 ccgtaccgac ggcggcgtcc atgccctgca aaactatttt cactttgact atgaaggaat
     3421 tatagaaaac cgagtgctct ataatataaa cgccatcctc cccccggaca tcgtcctgac
     3481 aggcattcac cccgtcgccc ccgacgccca ctgccgcttc gacgccatca gccgggagta
     3541 ccggtactat atctatcggg taaagaaccc ctttctcgca gaccgcgcct ggttctaccc
     3601 atataccctt gatcgccaga
//
LOCUS       DATGFG010000764         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_926524, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000764 DATGFG010000000
VERSION     DATGFG010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926524"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VKQ52_14340"
     CDS             <1..1129
                     /locus_tag="VKQ52_14340"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLZ88430.1"
                     /translation="IKGVPGSGCYGHNGADDVAADAALLAMAVPGKHVHLQWSREDEH
                     AWEPYGSAMVMELEALIQPDGKITHWKYALWSDTHGARPGGDAANLLTAHYLENPINK
                     KPSGFSGGAYRNADPYYAIPNRLVTANFFDGPLRTSSLRSLGAYANIFAIESFIDELA
                     IHAGKDPFEFRIQNLEDDRATAVLRQLQKQLAASPATHAVPTLATHTDPTSATPASPT
                     PTTHSIGTGIAFSRYKNSGSWCAVAARVSVDKTTGIVSVNKMWAVIDAGEVINLDGIK
                     NQIEGGMIQSASWTLHEQVKFDGKQVTSRQWATYPVMHINQAPEVEVTVIPRPQEPPL
                     GAGEAAQGPAAAAIVNAVYNACGVRVRSLPAGKIVLTSAPK"
     gene            1135..2610
                     /locus_tag="VKQ52_14345"
     CDS             1135..2610
                     /locus_tag="VKQ52_14345"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HLZ88431.1"
                     /translation="MQSLRPTYWLTRFLILRLLGVVYAVAFLAAINQVIPLIGSNGLL
                     PADTFFRRVSDALGSTAGFIRLPSLFWFTHSDAALLTVAWLGFLLSLAVAAGFANSIM
                     LFILWFLYMSFVNAGQDWYGYGWEIQLLETGFLAIFLCPLLDPRPFPKRAPPLPIIIL
                     FRWLICRIMLGSAFIKLRGDEIWRNGTALYYHFETQPLPGPLSRWFHFLPHFILRLGV
                     WYNWLAEGIAAFFVFWPRIARHIAGIIIIGLQVILIFSGNLSFLNWLTIIPALACFDD
                     GFWAKLLPRSLVRRAEAAEEKAEPSRSMTTIAWSITVIIGLLSIQPVINMLSPQQIMN
                     TSYDPLDLVNTYGAFGSVGQERLNVVFEGTANDTTANAVWKAYPYKALPAAPDKRPPQ
                     VAPYQPRLDWQMWFAAMSTPGEYPWTLTLIAKLLHNDPGALSLFAANPFPDKPPRYIR
                     AVLYRYSFAKPGNPEHRWWDRTPIAIWLPPMSANDPRLLIH"
     gene            complement(2623..>3214)
                     /locus_tag="VKQ52_14350"
     CDS             complement(2623..>3214)
                     /locus_tag="VKQ52_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HLZ88432.1"
                     /translation="SLDYVLTAAKRWANHRIDDFTLQIDAGEFQDLSIARTFFQHASE
                     WTMSGVGKGLDWAAEKGEDKKTGRAEFFIRSGMLVFKKANFKPDGELHLNAVNSYFYY
                     TKTLDKHTGDGPFKFDYRIDDLPYAIEGDNTLRDAVDELSNKIRRNLPFARRGYIFKS
                     PEIQAYYARQPWYVPDSSYVPVVNQLTRKEQEMVNK"
BASE COUNT          681 a         1198 c          700 g          635 t
ORIGIN      
        1 cataaaaggc gtacccggtt ccggctgcta tggacacaac ggcgccgatg acgtagccgc
       61 cgacgctgcc ctgctggcaa tggccgtacc gggaaaacac gtgcacctgc aatggtcacg
      121 cgaagacgaa cacgcatggg aaccctatgg cagcgccatg gtgatggaac tggaagccct
      181 gatccagccc gatggaaaga tcacccactg gaaatatgcc ctttggtcgg acacccatgg
      241 agcccggccg ggcggcgacg ccgccaacct gctgaccgcc cattatctcg aaaacccaat
      301 taacaagaag ccctccggct tcagcggcgg cgcctatcga aatgcagacc cctattacgc
      361 catccccaac cggctggtaa cggcaaattt tttcgacggc ccgctgcgca cctcctccct
      421 gcgcagcctg ggcgcctacg ccaacatctt cgccatcgaa tccttcatcg atgagctggc
      481 catacacgca ggcaaagacc ccttcgaatt ccgcatccaa aacctcgaag acgaccgcgc
      541 caccgccgtc ctccgccaac tgcaaaaaca actggccgct tccccggcaa ctcacgccgt
      601 cccaacactc gcaacgcaca ccgacccaac ctccgcaaca cccgcctccc caactcccac
      661 aacccattcc atcggcaccg gcatcgcctt ctcccgctat aaaaactccg gctcctggtg
      721 cgcagtcgcc gcccgggtat cggtcgacaa aaccaccggc atcgtcagcg tcaacaaaat
      781 gtgggccgtc atcgacgccg gggaagtcat caatctcgat ggaataaaaa accagatcga
      841 aggcggcatg atccaatccg ccagctggac cctccatgaa caggtaaagt tcgacgggaa
      901 acaagtcacc agccgccaat gggccaccta tcccgtcatg catatcaacc aggcccccga
      961 agtggaagtc accgtcatcc cccgcccaca ggaaccccca ctgggagcag gcgaagccgc
     1021 acaggggccc gctgccgccg ccatcgtcaa cgccgtatac aacgcctgcg gggtaagggt
     1081 ccgctcactc cccgccggca aaatagtcct aacttcagca ccaaagtagt ggatatgcag
     1141 agccttcgcc caacctactg gctcacccgt ttcctcatcc tccgcctcct cggggtcgtc
     1201 tacgccgtgg cctttctcgc cgcgataaac caggtcatcc ccctcatcgg ttccaacggt
     1261 cttcttcccg ccgacacttt cttccggcgc gtcagcgacg cactcgggtc gacagccggc
     1321 ttcatccgcc tcccatccct tttctggttc acccattccg atgcggccct cctcaccgtc
     1381 gcctggctcg gcttcctcct ctccctggcc gtagcagccg gctttgccaa ttcgatcatg
     1441 ctctttatcc tctggttcct ctatatgtcc tttgtcaacg ccggtcagga ttggtatggc
     1501 tacggctggg agatccaatt gcttgaaacc ggcttcctcg ccatcttcct ctgccccctc
     1561 ctcgacccgc gccccttccc aaaacgcgcc cctcccctcc ccatcatcat cctgttccgc
     1621 tggctcatct gccgcatcat gctgggctcc gcattcataa aattgcgcgg cgacgaaata
     1681 tggcgcaacg gcaccgccct ctactaccac ttcgagaccc aacccctgcc cggcccgctg
     1741 agccgctggt ttcattttct tccacatttc atcctgagac tcggcgtctg gtacaactgg
     1801 ctggcagagg gcatcgccgc cttcttcgtc ttctggcccc gcatcgcccg ccacatagcc
     1861 ggcatcatca tcatcggcct ccaggtcatc ctcatcttca gcggaaacct ctccttcctc
     1921 aattggctaa ccatcattcc cgcactggcc tgtttcgacg atggcttctg ggccaaactc
     1981 ctcccccgct cactcgtccg ccgcgcggaa gccgcggaag aaaaagccga accatcccgg
     2041 tccatgacaa ccatcgcctg gtccatcacc gtcatcatcg gcctgctcag tatccaaccc
     2101 gtgataaata tgctgtcacc ccaacagatc atgaacacct cctacgatcc cctcgatctc
     2161 gtcaatacat acggcgcctt cggctcggtc ggccaggaac gcctgaatgt cgtattcgaa
     2221 ggcaccgcca acgacacaac cgctaatgcc gtctggaaag cctatcccta caaagccctc
     2281 cccgcggccc ccgacaaacg cccgccacag gtagccccct atcaaccccg cctggactgg
     2341 caaatgtggt tcgccgccat gtccacaccc ggcgaatacc catggacgtt aactctcatc
     2401 gcgaaactcc tgcacaacga ccccggtgca ctaagcctct tcgccgccaa ccccttcccc
     2461 gataaaccac cacgctacat ccgcgccgtt ctctatcgct actccttcgc aaaacccggc
     2521 aaccccgaac accgctggtg ggatcgcacc cccatcgcca tctggctgcc ccccatgtcc
     2581 gccaacgatc cccgcctcct catccattaa cccgggaaca ccctatttat tcaccatctc
     2641 ctgttcctta cgcgtaagct gattcaccac aggtacataa ctcgagtccg gcacatacca
     2701 gggctgccgc gcataatacg cctggatctc cggcgacttg aaaatatatc cccttctcgc
     2761 aaaaggcagg ttccgcctga tcttgttcga tagctcatca accgcatccc gcagggtgtt
     2821 gtccccctcg atggcataag gcagatcgtc gatccggtag tcgaatttga atggcccgtc
     2881 acccgtatgt ttatccagcg tcttcgtata ataaaaataa gaattcacgg cattgagatg
     2941 aagctcgccg tcaggtttga aattcgcctt cttaaaaacc agcatcccgc tgcggataaa
     3001 gaattcagcc ctgccggtct ttttgtcctc acccttctcc gccgcccaat cgagcccctt
     3061 tccaaccccc gacatcgtcc actctgaagc atgctggaaa aaggtcctgg ctatcgaaag
     3121 gtcctggaac tcccccgcat cgatctgcag cgtgaaatcg tctatccggt ggttggccca
     3181 acgtttcgcc gccgtgagta catagtccag gctg
//
LOCUS       DATGFG010000765         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_636808, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000765 DATGFG010000000
VERSION     DATGFG010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636808"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1823)
                     /locus_tag="VKQ52_14355"
     CDS             complement(<1..1823)
                     /locus_tag="VKQ52_14355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88433.1"
                     /translation="MLKNSLTIAWRRLLKDRQFTLLNLLGLSTGLACAILIYCWVSDE
                     LGVDRYNSNDLRLYQVLLNSPSADGHEITTADYTPGLLASTLVKEFPDVEAAVSVVPS
                     PWHDKKGILSTGRQSIQTNGIFIGKAFFDIFTLPLIAGDKQNILRDRNDIVISTDLAR
                     ELFGTTDNLIGKTLEWDKKEYTGLYRISGIFQKPPANATLQFDLAFNYDLFLDKNQKL
                     RSWRNNDPSTYILLKKTADIGKLNNKIAGLIKNKDAEARSTLFAQQYSRRYLHGVYEN
                     GHAAGGRIEYVRLFSIIAIFILVIACINFMNLSTAKAAGRVKEAGIKKVIGANRAALI
                     GQYLAESLLLTAIAVLIAIGLAALALPQFGHITGKQLQLHFDSPFIGIILAITLLTGL
                     ISGSYPALYCSSFKPAASLAGKLPNTISEFLVRKGLVVFQFTLSTLFIVAVLVVHRQM
                     DLVQTKDLGYNRNHLLYFNSPYDSSLLRLLQQVKNTPGVLDSANFGQNITNRDGGTYD
                     ISWPGKDPNAKIVFTDLAIGYNFIETAGMKMKAGRSFSPAYGTERSSIIFNQAAIDIM
                     GVKDPVGKTVHLWGEDRHIVGVVENFNFQSLHEAIKPCFFE"
     gene            complement(1926..>2798)
                     /locus_tag="VKQ52_14360"
     CDS             complement(1926..>2798)
                     /locus_tag="VKQ52_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HLZ88434.1"
                     /translation="RRPLPAPPRRDPRPRRLLPYPLRRLRFFNARVFDQVELNYIQLQ
                     TIFRQKDETFKKLLNSIRENNINDEELALLNTRYLPDHTPPPGDLRLTLTTTNKVADA
                     INTERMKNLPGKPHHFYAVTDGNKDPNSYPADPTLILKKGAQVMFLKNDPEKRWVNGT
                     LGRVTAIGKDRITVRVGKHSYPVERATWESVDYRYDAKKQNIETATAGSFTQFPLRPA
                     WAMTIHKSQGRTFDQVIIDLGPGAFTHGQTYVALSRCTSLAGIVLKTPIRREDLILDP
                     GVKQFIAKRSKPAA"
BASE COUNT          491 a          559 c         1056 g          692 t
ORIGIN      
        1 cattcgaaga aacagggttt tatggcttcg tggagggact ggaaattgaa gttctccacg
       61 actccgacga tgtgtctgtc ttcaccccag aggtggaccg ttttgccgac cggatccttt
      121 actcccatga tgtcgatggc cgcctggttg aagatgatgg atgacctttc cgtgccgtat
      181 gcgggggaga aggatcggcc ggctttcatt ttcatccccg cggtctctat gaaattatat
      241 ccgatggcca gatcggtgaa aactattttt gcgttggggt cttttcccgg ccaggagata
      301 tcgtatgtgc cgccgtcgcg gttggtgatg ttctggccga agttggcgga gtcgaggacc
      361 ccgggggtgt tctttacctg ttgcaggaga cggagcaggg aggagtcgta ggggctgttg
      421 aagtagagaa gatggttgcg gttgtagcca agatcctttg tctgcaccag atccatttgc
      481 cggtggacga cgagtacggc gacgatgaag agggtggaga gagtgaactg gaaaacgacg
      541 agcccttttc gtacgaggaa ctcgctaatg gtgtttggca gtttgccggc gaggctggcg
      601 gcgggtttga agctgctgca atagagggcg gggtagctgc cggagatgag gcctgtgagg
      661 agggtgatgg cgaggatgat cccgatgaag ggactgtcga aatggagttg gagctgttta
      721 ccagtgatgt ggccaaactg cgggagggcg agggcggcca ggccgatggc gatgaggacg
      781 gcgatggcgg tcagcaggag ggactccgcg aggtattgcc cgatgagtgc ggcgcggttg
      841 gcgccgatca ccttttttat gcctgcttct tttacccggc cagcggcttt ggcagtggac
      901 aggttcatga aattgatgca ggcgatgacg aggatgaaga tggcgatgat ggagaacagg
      961 cggacatatt ctatcctgcc gccggcggcg tgtccgttct catagacgcc atggaggtaa
     1021 cgccgggaat attgctgtgc gaacagggtc gaccttgctt cggcgtcctt gttttttatg
     1081 aggccggcta ttttattatt gagcttgccg atgtccgcgg tctttttcaa aaggatatag
     1141 gtggacggat cgttgttgcg ccagctccgg agtttttggt tcttatcgag gaagaggtcg
     1201 tagttaaagg cgagatcgaa ttgcaacgtc gcgttggcgg gtggtttttg gaagataccc
     1261 gagatgcgat agaggccggt atactctttt ttgtcccatt cgagggtttt gccgatgagg
     1321 ttgtctgttg tgccgaagag ttcccgggcg aggtctgtgg agatgacgat gtcgttcctg
     1381 tcccggagga tgttttgttt gtcgcctgcg atgaggggga gggtgaagat atcgaagaag
     1441 gctttcccga tgaatatacc gttggtttgg atggactgcc ggccggtgga gaggatgccc
     1501 tttttgtcgt gccagggaga ggggacgacc gataccgcgg cttcgacatc ggggaactct
     1561 ttcaccaggg tagaggcgag gaggccgggg gtatagtcgg cggtggttat ttcgtggccg
     1621 tcggcggagg gggagttgag caggacctgg tagagacgga ggtcgttgct gttgtatcgg
     1681 tctactccga gctcgtcgct tacccaacag tagatgagga tggcgcaggc aaggccggtg
     1741 gagaggccca ggaggttgag gagggtgaac tggcggtctt tgaggaggcg gcgccaggcg
     1801 atggtgaggc tattttttaa catggagggt tctttggcag aagaaagacg gggctgggcg
     1861 gggaatgttg catgggccgg gagcggatgc cggaggagtt aatggggcgc gcgtttgggg
     1921 cctggtcagg cggcgggttt ggatcgtttt gcgatgaatt gttttacgcc ggggtcgagg
     1981 atgaggtctt cgcggcggat gggggttttg aggacgatcc cggcgaggct tgtgcagcgg
     2041 ctgagggcga cataggtctg gccgtgggtg aaggcgccgg gaccgagatc gatgatgacc
     2101 tggtcgaagg ttctgccctg gcttttgtgg atggtcatgg cccaggccgg gcgtaggggg
     2161 aactgggtga aggagccggc ggtggctgtt tcgatgtttt gtttttttgc atcgtagcga
     2221 tagtcgacgg actcccaggt ggcgcgttcg acgggatagg agtgtttgcc gacgcggacg
     2281 gtgatcctgt ctttgccgat ggcggtgacg cggccgaggg tgccgttgac ccagcgtttt
     2341 tcggggtcat ttttcaggaa catcacctgt gcgccttttt tcaggatgag ggtggggtcg
     2401 gcggggtagg agttggggtc tttgttgccg tcggtgacgg cgtagaaatg gtgtggtttg
     2461 ccggggaggt ttttcatcct ttcggtgttg atggcatcgg ctactttgtt tgtcgttgtg
     2521 agggtgaggc ggaggtcgcc gggtggcggt gtatgatcgg ggaggtagcg ggtgttcagc
     2581 agcgcgagtt cctcgtcgtt aatgttgttc tcgcggatgc tgttgaggag ttttttgaag
     2641 gtttcgtcct tttggcggaa gatggtctgg agctggatgt agttgagttc cacctggtcg
     2701 aagacgcggg cgttgaagaa gcggagccgc cgaagcgggt atggaagtag ccggcgaggt
     2761 cgcgggtcac ggcggggagg agctggtaga ggtcgccg
//
LOCUS       DATGFG010000766         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_699318, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000766 DATGFG010000000
VERSION     DATGFG010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699318"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4340
                     /locus_tag="VKQ52_14365"
     CDS             <1..>4340
                     /locus_tag="VKQ52_14365"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HLZ88435.1"
                     /translation="TNSSASESANLAFTVGFTIFGVKFTVNPSANIGDGMARELIKMQ
                     DINGDGYPDYISSTQDDNLTVALSTIGRTNLLRTVNRPMGAAFTLDYQRVGNTYAMPN
                     SVWTLASVKVFDGFKGDGPDTLLTTFDYSGGNFDRDEREFYGFATVKTYSHDAAHNNV
                     VYSIGIDSFANDNYYTKGMVLSSYVQSGDGKLYDETVNTYELRDISTGTVLPDSYKTN
                     DAGAAFVALSRTDRLFYEGQPNPGKSTFVTYGYDTKGNITQYTDFGDAGTADDVSSTI
                     TYYNVPDKYIVSTPKSILVTGSGATYRKRECTIDTQTGDVTEIRQYLADATVAVTDMG
                     YDGYGNMINVTGPANARGQRFRLDYIFDDQVHQFILKASNSYGYASQATYDYRFGHPL
                     GTVDLNNNSVRYQLDDLGRVIQITGPYELAAGVPYTLRFDYHPASAVPWAHTAHYDPA
                     HPGNDLETVTFMDGLGRPLLTKKDAAIFQGRDKADVEQMIVSGRILFDGLGRQVATYY
                     PVTEDKGSDSVFNQSFDNISPTLTTYDVKHRVLTTTLPDGSVTQNTYGFGSDRQQQTQ
                     FSTKTQDANGKISEQLTNVRHLTMAQKRYTGNGNVWVSSTYDAINQQLTSTDDIGATI
                     SYQYDMLGRQVSRTHPDEGTSQFTYDLAGNMTKKVTANLQKESAAITYSYDFTRLTDV
                     TYPHNPEDNVHYTYGAPGAAYNRAGKVVVQEDASGAQEFFYGPLGEMMKNVRTIVIPR
                     FGQRTYVTQWNYDTWNRLTSMIYPDSEVISYTYNAGGHLLSMGGNRYGQATTYVQQLG
                     YDKFETRVYLGYGNGTQTSYTYEPDRRRLENLLTTVGNGRRVMDNTYSYDKVDNILGI
                     SNNAPVPGSNLMGGSSQYSYAYDDLYRLTTAAGSFKGPHEQDRYGMAMEYNTVGGITR
                     KTQTNDKSPNGNNKWIPQKKTTYDLSYNYGTQQPHAPTHIGRQTYTYDADGNQMGYTD
                     DLTGQRQVNVWDEENRLRSVSVNGQLNSYVYDAEGERVLKGVGAGETVYVNGAVSGSS
                     GGVGNFTVYVNPYLVVKSGEYTNHYFVESQRIATRLKQSWDQQVGAPDAGDSIPWAKK
                     GKALMLGIYRDQQALGGNDSAHAADITGINARMAAGNSVSGNGSAPGSNADTANNGNH
                     YAYGHYKKTGNGSTGSSDFLYFYHSDHLASTSYVTDGSGEVYQHLEYFAFGETFVDEH
                     SNTDRIPYLFNGKELDEETGLYYYGARYYDPRTSIWQSVDPMAVKMPAWSPYSMNYDN
                     PVKFVDLQGEKPTPAEAARMAAHVYGDKKDDILIGGWRVSKRNFGVTLKDDKTGLKSQ
                     VYERVVNGKVTEYVYATAGTEASWKDVKADAVQPLGFSKQYYEAADNAKTISRDLAKT
                     NTELTFTGHSLGGGEAALEALVTDRSAITFNAAGVGDITKFVAGTWKTPFKSEDKIDA
                     YIMATDPLNK"
BASE COUNT         1109 a         1113 c         1263 g          855 t
ORIGIN      
        1 taccaacagc agcgcttcgg aatcagccaa cctggccttc acggtcggtt ttaccatctt
       61 cggggttaaa ttcacggtga acccaagcgc caacattggc gatgggatgg cgcgcgaact
      121 gatcaagatg caggacatca acggcgacgg ctatcccgat tatatcagct ccacccagga
      181 tgacaacctc acggtagcct tgtcgactat cggccgtacc aatctgctcc ggacggtgaa
      241 ccggccgatg ggcgccgcct tcaccctgga ttaccagcgg gtgggcaata cctatgcaat
      301 gccgaacagt gtttggacgc tggcgtcggt gaaggtgttc gatgggttca aaggagatgg
      361 cccggatacg ttgctgacca cattcgacta ttcgggtggt aatttcgacc gggacgaacg
      421 ggagttctat ggattcgcga ccgtgaagac gtacagccat gatgccgcgc ataacaatgt
      481 ggtgtattcc atcggcattg acagctttgc caatgacaac tattacacaa agggtatggt
      541 gttgtcctcg tatgtgcaaa gcggggatgg gaaactatac gacgagacgg tgaacaccta
      601 cgagctgcgg gatatcagca cgggaacagt gttgcccgat tcctataaga caaatgacgc
      661 cggcgcggca tttgtcgcgc tgagccgtac agaccggctt ttttatgaag gccagcccaa
      721 cccggggaaa tcgaccttcg tcacgtatgg ctacgataca aaaggaaata tcacacagta
      781 taccgacttt ggggatgcgg gcacggcaga tgatgtcagt tcgacgatca cctattataa
      841 tgtgcccgac aagtatatcg tcagcacgcc gaagagcatc ctggtgacag gatcgggagc
      901 gacgtatcgc aaacgggagt gtaccatcga cacgcagaca ggggacgtaa cggagattcg
      961 gcagtacctg gctgatgcaa cggtggctgt caccgacatg ggctacgacg gctatggtaa
     1021 tatgatcaat gtcaccggtc cggccaatgc caggggccag cgtttccggc tggattatat
     1081 tttcgatgac caggtgcatc agtttatcct gaaggccagc aatagctacg ggtatgcctc
     1141 ccaggccacc tatgactata ggttcggtca tccgcttggc accgtggatc tgaataataa
     1201 ttccgtccgg tatcagttgg acgatctggg tcgggtaatt cagataacag gtccgtacga
     1261 actggctgcc ggggtgcctt ataccctccg gtttgactat catccggcgt cggccgtacc
     1321 atgggcgcat accgcgcact atgatccggc tcatccgggc aacgacctgg agacggtgac
     1381 ctttatggat ggccttggcc gcccgctgct gacgaagaaa gacgcggcta ttttccaggg
     1441 cagggataag gccgatgtgg aacagatgat cgtgtcgggc cgcatccttt tcgacgggct
     1501 tggacgacag gtggcaacct attaccccgt gacggaggac aagggcagcg atagcgtgtt
     1561 caaccaaagc tttgacaaca tcagcccgac gctaaccacc tatgatgtca agcaccgggt
     1621 gttgaccacc accttgccgg acggctcggt gacgcaaaat acgtatgggt ttggcagcga
     1681 ccggcagcag cagacccagt tcagcacgaa gacccaggac gcgaatggca agatctcgga
     1741 gcagttgacg aatgtccggc atctgacaat ggcgcagaag cgttataccg gcaacgggaa
     1801 tgtctgggtc agcagcacct acgacgctat caatcagcaa ctgacctcta ccgatgatat
     1861 cggcgccact atttcctatc agtacgatat gctgggacgg caggtgagcc gtacccatcc
     1921 ggatgaagga acgagccaat tcacctatga cctggcaggc aatatgacga aaaaggtgac
     1981 cgccaatctt cagaaggaaa gcgcggccat aacctacagc tatgatttca cgaggctgac
     2041 ggatgttacg tatccccata acccggagga taatgtacac tatacgtatg gcgcgcccgg
     2101 cgccgcctac aaccgggcgg gcaaggtcgt agtgcaggaa gacgcctcgg gggcccagga
     2161 atttttctac ggcccgttgg gagaaatgat gaagaacgtc cggacgatcg tcatcccgag
     2221 attcggccag cgcacctatg tgacgcaatg gaactatgac acctggaaca ggcttacctc
     2281 gatgatctat ccggacagcg aggtgatcag ctatacatat aatgccggcg gacatctgct
     2341 gtcgatgggc ggcaaccggt atggccaggc gaccacctac gtgcagcaat taggttatga
     2401 caagttcgag acccgtgtgt atcttggcta tggcaatggc acgcaaacca gctatacgta
     2461 tgaacccgat cgcaggcggc tggagaacct gctcaccacc gtggggaatg gacggcgcgt
     2521 catggacaat acctattcgt acgacaaggt ggacaatatc cttggtatct ctaacaatgc
     2581 gcctgtgcct ggcagcaacc tgatgggcgg gtcttcgcaa tacagttatg cgtatgatga
     2641 tctttaccgg ctcaccacgg ctgccggctc tttcaagggg ccccatgagc aggaccgcta
     2701 tggcatggcc atggagtaca ataccgtagg cggcattacc cggaagacac agaccaacga
     2761 caagagcccc aatggaaaca acaagtggat cccgcagaag aagaccactt acgacctgtc
     2821 gtacaattat ggtacacagc aaccacatgc gcccacgcat atcggccgcc agacctatac
     2881 ctatgacgcc gatggcaacc agatgggcta caccgacgac ctgacggggc agcggcaggt
     2941 aaatgtgtgg gatgaggaaa accggttgag gagcgtatcg gtgaatggac agctcaacag
     3001 ctatgtctat gacgcggaag gagagcgggt attgaaagga gtaggcgcgg gagaaacggt
     3061 ctacgtcaac ggcgccgtta gcggcagctc gggcggggtc ggcaatttca ccgtgtatgt
     3121 caatccctac ctggtggtga agagcggcga atacaccaac cactacttcg tcgaaagcca
     3181 gcgcatcgcc acccggctga agcaaagctg ggaccagcag gtgggcgctc ctgatgccgg
     3241 agacagtatt ccatgggcga agaaagggaa ggccctgatg ctgggcatct accgtgacca
     3301 gcaggccctc gggggtaacg acagtgcgca tgcggcggac atcacgggaa taaatgcgag
     3361 aatggcggcg ggcaacagtg tttcgggcaa cggcagtgcg cccggcagca acgccgatac
     3421 ggccaacaac ggtaatcatt acgcctacgg ccattacaag aagacaggca atggcagcac
     3481 gggcagctcg gattttctgt acttttatca ttccgaccat ctggcaagca ccagttacgt
     3541 cacggatggc agcggcgagg tctaccagca tctggaatat tttgcctttg gcgaaacatt
     3601 cgtggatgag cacagtaata cagacaggat tccttacctt ttcaatggga aggagctgga
     3661 tgaagagacc gggttgtact attatggcgc gaggtactat gatccgagga ccagtatctg
     3721 gcagagtgtc gacccaatgg cggtgaagat gccggcctgg agtccttact cgatgaacta
     3781 tgataacccg gtgaagttcg tcgatcttca gggtgagaag ccaacgcctg cggaagcggc
     3841 gagaatggcg gctcacgtat atggggacaa gaaggatgat atcctcatcg gcggctggcg
     3901 agtgtcgaag cggaattttg gggtcacgct taaagacgat aagacaggct taaagtcaca
     3961 ggtttatgaa cgagtagtaa acggaaaagt gacggaatat gtctatgcca cggccgggac
     4021 ggaagccagt tggaaggatg ttaaagccga tgcagtgcaa cctttgggtt tttcaaaaca
     4081 gtattatgag gctgctgaca atgccaagac aataagccgt gatcttgcaa aaacgaatac
     4141 ggagcttacc tttacaggtc actccctggg tggcggcgag gcggcgctgg aggcattggt
     4201 caccgacaga agtgcaatca ccttcaatgc ggccggtgta ggtgatataa caaaatttgt
     4261 tgcaggcaca tggaagacac catttaaatc tgaagacaag atcgacgcct acattatggc
     4321 cacagacccg ctgaacaaga
//
LOCUS       DATGFG010000767         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_229590, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000767 DATGFG010000000
VERSION     DATGFG010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229590"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VKQ52_14370"
     CDS             <1..587
                     /locus_tag="VKQ52_14370"
                     /inference="COORDINATES: protein motif:HMM:NF019929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HLZ88436.1"
                     /translation="HFDAIKRINTILIDLCRDIWTYVSMDYFKQKVKKGEIGSSAMPH
                     KVNPIDFENAEGNLGVANALFEHFSSKLPVSRLQRDLTDSTVLRNVGVPFAHTILALK
                     SIEKGLDKLVLNKERLVQDLENNWAVVAEAIQTVLRRENYPKPYEALKELTRGRTGID
                     KHAIHEFIDGLKVSATVKKELKRITPFNYTGVSF"
     gene            771..1259
                     /locus_tag="VKQ52_14375"
     CDS             771..1259
                     /locus_tag="VKQ52_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88437.1"
                     /translation="MKRILLFSSILICLYFTMSCTKTNNVTTTIRDTTVVRDTTVLRD
                     TVYLSPKYPIAGHWVGSYFLSGDLVDSFMYSFDIRDDGTVYTIGSGTNQTAGYASGPW
                     TLSGVNFSATLTTMAGVSVENIQTVTARYDSVTGRLYNGVWIDTRGNGGQTGTFSLRR
                     VQ"
     gene            complement(1329..2066)
                     /locus_tag="VKQ52_14380"
     CDS             complement(1329..2066)
                     /locus_tag="VKQ52_14380"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HLZ88438.1"
                     /translation="MKSNRSFWLVFAAFLALASCFKRSIPIPHPPATDSVVTVNDTDQ
                     TLILRPDSTTGQDSYVSKLDDYPTDGNVNLNFAHEMVLARWVFSQDNYDSATQRGYIR
                     FDSLRKIPSTATVTSAILYLYGESTSLSYPYGNSYYPGSYNPPNPGLIQRVVGGTWDQ
                     KTITWNNAPALTSVGQDTIPASTSAFNYNVAVDVTSLVRTMVDSPATNYGFGLRLITE
                     QASRTMQFGSCEVPDSTLRPKLVVRYH"
     gene            complement(2128..3717)
                     /locus_tag="VKQ52_14385"
     CDS             complement(2128..3717)
                     /locus_tag="VKQ52_14385"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HLZ88439.1"
                     /translation="MRYTNEINKRKTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVK
                     SNKIKKHATSDFMEIERQRGISVATSVMSFEYKGMLINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDSVNGVEAQTRRLMEVCRMRDTPVIVFINKMDRDGKNRFDLLEEVEKELN
                     IQLHPMTWPINSGKDFKGVYNLYDKNLVLFTANTKAVDEDDLIRIDRLDSGLLDEKLG
                     EKDAAVLREDVELVDGVHGQLNVGDYLNGSVAPVFFGSAINNFGVREMLDTFIRIAPA
                     PRGRQTSTRQLDAGEDKFSGFVFKIHANLDPKHRDRIAFLRVCSGRFERNKYFHHVRL
                     DKDVRFSNPYTFLARDKDVIEEAYPGDVVGLFDTGNFKIGDTLTEGEDFFFTGIPSFS
                     PEIFKEVVNKDPMKTKQLEKGLLQLTDEGVAQLFTQYGGNKKIIGCVGELQFEVIQYR
                     LLHEYGASVQFNAQPFYKACWITSNDPKKLEDFTRFKHANIAEDKDGYLVYLAQSEWF
                     LNTERTNNPEIEFHFTSEIHK"
     gene            complement(3740..>4476)
                     /locus_tag="VKQ52_14390"
     CDS             complement(3740..>4476)
                     /locus_tag="VKQ52_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88440.1"
                     /translation="GIYTLAQNIASLIQAPQRGVIAASIGPLSKAWKDKDLGKINRIY
                     KRSSINQLIFSVALFILIWINFDDAVRTFHLKSDYLLARNAFLFIGLMRVVDMGTGVT
                     NQIIGTSTYWRFDFYTGVTLAILTLPMNYLLTKTIGFTGPAIADLITFSVYNGIRWVF
                     LYRKFHMQPFTMKTLYTLLLGVAAWWVSQLLFSAEGGFLLMTARSVVVLLLFGGGVLS
                     LRLSEDILPVWDTVKKRLGLGSAATR"
BASE COUNT         1018 a         1178 c         1215 g         1065 t
ORIGIN      
        1 cccattttga tgctattaaa cgaatcaata ccatcctaat cgatctatgc agggacatct
       61 ggacctatgt ctcgatggat tatttcaaac aaaaagtgaa aaagggtgag atcggctcat
      121 cggcaatgcc ccataaagtg aatcccatcg actttgagaa tgcggaaggc aacctcggtg
      181 tcgccaatgc cctgttcgag catttttcct cgaaactgcc cgttagccgc cttcaacggg
      241 atctgaccga ttccaccgtt ctgcggaatg tgggcgtccc ctttgcgcat accatcctgg
      301 cgctgaaatc tattgaaaaa ggcctggaca agctggtgct gaacaaggag cggctggtac
      361 aggatctgga gaacaactgg gccgttgttg ccgaagccat tcaaacggtc ctgcgccgcg
      421 agaactatcc caaaccttat gaggcactaa aggaactcac gaggggccgg acgggcatcg
      481 acaagcacgc catccacgag ttcatcgatg gactcaaggt ctcggccacg gtaaaaaagg
      541 aattaaaacg catcactccg ttcaattaca cgggggtcag tttctaattt ttttccactc
      601 tccggaatac ttaaaatttg cggtcaaatg gacggttttc gtgtccaaat cctggatatt
      661 cgtgtcaaat caaccgtcta actcgttgaa aatatgccct atacctagta cgatatttca
      721 ttctaagaag aatatgtact acttttcgac ccttaacaaa tacccctaat atgaaacgca
      781 ttctgctgtt ttcttctatc ctgatctgtc tttactttac catgtcctgt acgaaaacca
      841 acaacgttac gacaacgatc agggatacca ctgtggtccg ggataccacc gttctgcgtg
      901 acaccgttta tttgtcgcca aaatatccca tcgccggcca ttgggtaggg tcctatttcc
      961 ttagcggtga tctcgtggac tccttcatgt attcctttga cattcgtgat gacggcactg
     1021 tctacaccat cggaagcggt accaatcaga ccgccggata tgcttcgggc ccctggaccc
     1081 ttagcggcgt caacttctcg gcaaccctga ccactatggc cggcgtttcg gtggagaata
     1141 ttcaaaccgt taccgccagg tacgacagcg ttacgggaag gctttacaat ggagtatgga
     1201 tagacacccg tggaaatggc ggtcagacgg gcactttctc cctcagacgg gttcaataac
     1261 aagaatacct aaactgcgat atagcacaag cgtccaccgt ccgggaacgg tggacgcttt
     1321 cttatttcct aatgatatct tacgaccagt ttcggccgga gtgtcgagtc cggcacctcg
     1381 cagctgccga attgcatggt ccggctggcc tgctcggtga tcaaccgcag gccgaagcca
     1441 tagttggtgg cgggtgaatc caccatcgtc cgcaccaggc tggtgacatc gacggcgaca
     1501 ttataattga atgcacttgt ggatgcgggt atggtgtcct gcccgacgct ggtcagcgcc
     1561 ggggcattgt tccaggtaat ggtcttctga tcccaggttc ctcctacaac ccgttggatc
     1621 aggccgggat tgggcggatt gtacgagccg ggataatacg agttcccgta gggataggac
     1681 agggaagtgg attccccgta aaggtagagg atcgcggaag tgaccgtcgc cgtggatggg
     1741 atcttcctga ggctgtcgaa ccggatgtaa ccgcgctggg tagcggagtc atagttatcc
     1801 tgtgaaaaga cccagcgggc gagtaccatt tcatgcgcaa agttgaggtt gacattgccg
     1861 tcggtgggat agtcgtccag cttggaaaca taactatcct ggccggtggt gctgtcgggc
     1921 cgaaggatca gggtctgatc ggtgtcgttc accgtcacaa cagaatcggt ggcaggcgga
     1981 tgggggatgg gaatggagcg tttgaagcaa ctcgccagcg ccaggaaggc cgcaaaaacg
     2041 agccagaagg atcgatttga tttcataaga tacgaataga ttaggggata aacatacagt
     2101 atttcccccg atattgcaat cccgcgccta tttatggatc tccgaggtaa aatggaattc
     2161 tatttccggg ttgttggtcc tttccgtatt cagaaaccat tcactctgag ccaggtatac
     2221 gagatatcca tccttgtctt cggctatatt ggcatgtttg aaccgggtaa aatcctccag
     2281 ttttttgggg tcgttgctcg tgatccagca ggccttgtaa aaaggctggg cattgaactg
     2341 aacggaagcg ccatactcat gcaggagccg gtattggatc acttcgaact gcagctcccc
     2401 gacacagccg atgatcttct tgttgccacc atactgggtg aacaactggg cgaccccctc
     2461 atcggtcaat tgtaataacc ctttctccaa ttgcttggtt ttcatggggt ctttgttcac
     2521 cacctctttg aatatctcgg gggagaagga aggtatgccg gtaaaaaaga agtcttcgcc
     2581 ctccgtgagg gtatcgccga tcttaaagtt gcccgtatcg aacaggccca cgacatcccc
     2641 cgggtaggct tcttcgatca cgtccttgtc ccgggcgaga aaagtatagg gattgctgaa
     2701 ccggacatcc ttgtccaggc ggacatgatg gaagtatttg ttgcgctcga accggccgct
     2761 gcatacccgc aggaaggcga tgcggtccct gtgcttgggg tcgagattgg cgtggatctt
     2821 aaagacaaaa ccgctgaatt tgtcctcccc ggcatccagc tggcgcgtgg acgtctgccg
     2881 gccgcggggc gcgggggcga tcctgataaa ggtgtcgagc atctccctga ccccaaaatt
     2941 gttgatggcg ctgccaaaga acaccggcgc cacgctgccg ttcaggtagt ccccgacgtt
     3001 gagctgtcca tgcaccccat ccaccagttc cacatcctcg cggaggaccg ccgcgtcctt
     3061 ttctccgagt ttctcgtcca gcaggccgct gtccagccgg tcgatcctga tcagatcgtc
     3121 ctcgtccacg gcctttgtat tggccgtaaa aaggaccagg ttcttatcgt aaagattgta
     3181 tacccctttg aagtccttcc cgctgttgat gggccaggtc atcgggtgca attgtatatt
     3241 cagctctttt tcaacttctt ccagcagatc gaaccggttc ttgccatccc ggtccatctt
     3301 gttgataaag acgatcaccg gggtgtcccg catacggcag acttccatca gccggcgggt
     3361 ctgggcttcc accccattga cgctgtcgat cacgaggatg acactgtcca cggccgtgag
     3421 ggtccggtag gtgtcttcgg cgaaatcctt gtgacccggg gtgtccagga ggttgatcag
     3481 catccccttg tattcaaagc tcatgaccga ggtggccacc gagatacccc tttgccgttc
     3541 gatctccatg aagtcgctgg tcgcgtgctt cttgatcttg ttgcttttga cggccccggc
     3601 ggtctggatg gcgccgccga acagcaggaa cttctccgtc agggtcgttt tgccggcgtc
     3661 ggggtgggat atgatggcaa acgtcttgcg tttgttgatc tcgttcgtgt atcgcataaa
     3721 gtccgcaaag gtactaaatt tagcgggtgg cggcgctccc gagacctaac cttttcttca
     3781 ccgtatccca taccggcagg atatcctccg agagccggag actgagcacc cctccgccaa
     3841 agaggagaag aaccaccacg ctgcgggccg tcattaagag aaatccacct tcagcggaga
     3901 ataacagttg gctgacccac caggcggcga cccccagcaa cagggtatag agggtcttca
     3961 tggtgaaagg ctgcatgtgg aatttcctgt agagaaagac ccaccggata ccgttataaa
     4021 cggaaaacgt gatgagatcg gcaatggcgg gaccggtaaa cccgatcgtt tttgtcagca
     4081 ggtagttcat cggcagcgta aggatggcga gagtgacgcc ggtgtaaaag tcgaaccgcc
     4141 agtaggtgga cgtgccgatg atctggttgg tgacgcctgt ccccatgtcc acgacccgca
     4201 tcagcccgat gaaaaggaac gcattcctgg caaggaggta atccgatttc agatgaaagg
     4261 tgcgtactgc gtcgtcgaaa ttgatccaga tgaggatgaa cagggccacc gagaagatca
     4321 gctgattgat cgaggagcgc ttatagatgc ggtttatctt cccgaggtct ttgtccttcc
     4381 atgccttgga gagggggccg atggaggcgg cgatgacccc gcgctgcggc gcctgtatca
     4441 ggctggcgat gttttgggca agcgtataga tgccca
//
LOCUS       DATGFG010000768         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_354002, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000768 DATGFG010000000
VERSION     DATGFG010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354002"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="VKQ52_14395"
     CDS             complement(<1..1348)
                     /locus_tag="VKQ52_14395"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ88441.1"
                     /translation="MNKLLRSIIAFSLKNRYFIFFCTALLLVSGIITFRNMPIEAFPD
                     VTNTEISIITQWPGRSAEEVEKFITIPIEIAMNPVQQKISLRSTSIFGLSYVKLIFED
                     GVVDRDARNQVNALLANVSLPESIQPQVQPPTGPTGEIFRYTLKSDIRDDRELKTMQD
                     WVIDRQLRAVPGVADIVSFGGRVKTYEIQVDPGKLTNLGITPLDVYSAVQKSNINIGG
                     DFIVRNNQAYVVRGIGLLNDIHEIRNIIITNTNDIPVLVKDVAEVRISNLPRLGHVGR
                     ADHIVDSAGNTHVTDEDDVVEAIVIMRKGENPTQVVQAVKKKIDKLNDLVLPKDTRIV
                     PYYDRENLIHYATHTVLHNLVEGILLVTLLVSVFMFNWRTTVIVSIIIPLSLLFAFIC
                     LHLMGMSANLLSLGAVDFGIIIDGAVVMVEGLFVILDHQARDVGMERFNKMAKMGLI"
     gene            complement(1383..2492)
                     /locus_tag="VKQ52_14400"
     CDS             complement(1383..2492)
                     /locus_tag="VKQ52_14400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ88442.1"
                     /translation="MQKIFKAGSQFLPFLLAGSALLFQACGSQEQKPAGDKEKYVLPD
                     TIARSLKIDTVTISQLVNSITLTGKVGSDEDHVVPVNSMVSGNISDVKAMLGDYVQQG
                     QVLGVVRSSEMAGYSNDLVNAETNLRLAQTNLQKTKDLFKSGLASMTDSLNAEISVQQ
                     AQSELNRVHSVLKINGGGTQGEFVIKAPIGGFIVQKSAINNMTIRADNSNSLFTISDL
                     KTVWIQANVYESNISLIKQGENVDVTTLAYPGRTFKGKIDKIMNVLDPSSKVMKVRVV
                     LPNPDYLLKPEMYASITVSDKENAKCLSIPSQALIFDHSQYYVLVYNSKSDIKITPVQ
                     VLNTVGDRTYLASGVNNGDKVVGSQAILIYNALNS"
     gene            complement(2539..3813)
                     /locus_tag="VKQ52_14405"
     CDS             complement(2539..3813)
                     /locus_tag="VKQ52_14405"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ88443.1"
                     /translation="MHRKWLSLFAGLCFIGAAVTAQPRQDTLRLSLSSAEKMFLDSNL
                     QLLAQRYNIDASQALVLQARLWPNPNFSIGHTLYSGTLNEFFPLGRNDETTVGLSQLI
                     LLAGKRNKQVKLAQENVHLTEYQFFDLLRTLKYTLRTDFFQIYYLQQSAKVYDAEIKA
                     LSQIVNAFAEQQGKGYISEKEVVRIKAQLYSFQSEYSDLINQVNSTESELRLVLQVKP
                     NFFVDPEVDSAALTQLDPAKYPLTTLIDSAYHNRTDLQIARANTRINQLNYNYQKALA
                     VPDLTLSLGYDEAGSFLYNYYGIGASIDLPFFNHNQGNIKNAKLQIASTEATQRSTQA
                     TVEENVATSLQKAFAQQKLFQTIDPKFFADFQRLMHEMLANYQKRNISILEFLDFYDS
                     YKQNTLQLNNIGFNRVSAFEDINYYTGTNFFN"
     gene            complement(4070..4771)
                     /locus_tag="VKQ52_14410"
     CDS             complement(4070..4771)
                     /locus_tag="VKQ52_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88444.1"
                     /translation="MQQVSKVKVLLAEDDLSLGYVIKDNLVDAGYEVVLCPDGQAAIE
                     KFDKNEFGICLLDVMMPNKDGFAVARKIRQQSDLVPILFLTARSMEEDKIKGFQSGAD
                     DYITKPFSIQELLMRMEVFLRRTRKMHSDRTVNFTIGQLNFSYTDLKLTIGQEVFNLT
                     QKEADLLKFLCEHSNRILKREEVLLNVWGKDDYFLGRSMDVFITKLRKYFKADPQVNL
                     ETIHGVGFRFNTPQG"
     gene            complement(4781..>4975)
                     /locus_tag="VKQ52_14415"
     CDS             complement(4781..>4975)
                     /locus_tag="VKQ52_14415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88445.1"
                     /translation="IEKQYLKNIFKKFYRVPTGDIHDVKGFGLGLNFVKRIIDAHKGK
                     IRVNSLPGIGTEFRLFIPLN"
BASE COUNT         1072 a         1253 c         1370 g         1280 t
ORIGIN      
        1 tgatgaggcc catcttggcc atcttgttaa agcgttccat gccgacgtcg cgggcctggt
       61 ggtcgagaat aacgaagagg ccttccacca tcaccaccgc gccgtcgatg atgatcccga
      121 agtccacggc gccgagcgag agcaggttgg cgctcattcc cattaaatgc agacagataa
      181 aggcaaagag cagggatagc gggatgatga tggacacgat caccgtcgtg cgccaattga
      241 acatgaagac cgataccagc agggtaacga gcaggatacc ttccacgagg ttgtgcaaca
      301 cggtgtgcgt ggcatagtgg atcaggttct cgcggtcgta gtagggaacg atccgggtgt
      361 cctttggcaa taccaggtcg ttcagcttat cgatcttctt cttcacggcc tgcaccacct
      421 gggtggggtt ctcccccttc cgcatgatga cgatggcttc caccacatca tcctcgtcgg
      481 tgacatgagt atttcccgcg gaatcgacga tatggtcggc ccggcccacg tggccgagac
      541 ggggcaggtt cgagatgcgc acctccgcca cgtcttttac cagtacgggg atatcgttgg
      601 tattggtgat gatgatattc ctgatctcgt ggatgtcatt cagcaagccg atgccgcgga
      661 ccacataggc ctggttgttc cggacgatga aatcgccccc gatgttgata ttgctcttct
      721 gcacggcgga atagacatcc agcggcgtga tccccaggtt ggtgagcttc ccggggtcta
      781 cctggatctc gtaggtcttt acccggccgc cgaaactcac gatatcggcg actcccggca
      841 cggcacgcag ttgccggtcg atgacccagt cctgcatggt cttgagctcc cggtcatccc
      901 ggatatcgct tttcagcgta taacggaaga tctccccggt gggtcccgtg ggtggctgca
      961 cctggggttg aatactctcc ggcagggaca cattggccag gagggcgttc acctggttgc
     1021 gggcatcccg gtctacgacc ccgtcctcga agatgagctt cacataggaa agcccgaaga
     1081 tgctggtgct gcgcaggctg atcttctgct gaaccgggtt catggcgatc tcgatcggga
     1141 ttgtgatgaa cttctctact tcctccgcgc tgcggcccgg ccactgggtg atgatgctga
     1201 tctccgtatt ggtgacatcg gggaaggcct cgatgggcat attccggaaa gtgatgatcc
     1261 cgctcaccag cagcagcgca gtacaaaaga atatgaagta tctgttcttt aaggaaaagg
     1321 caataatact ccttagaagc ttattcatct tctgaaattt tgggatttta tgttaatatg
     1381 gcctagctgt tcaacgcgtt atagatcagt atcgcctgcg agccgacgac cttgtcgccg
     1441 ttgtttacgc ccgaagcgag ataagtccgg tcaccaaccg tattgaggac ttgtacagga
     1501 gtaattttga tgtccgactt gctgttgtac accagcacat agtactggct gtgatcgaag
     1561 atcagcgcct gggacgggat gctgagacat ttcgcatttt ccttgtcact aacggtaatg
     1621 ctcgcataca tctctggctt taacagatag tcggggttgg ggagtaccac gcgcaccttc
     1681 atcaccttgc tggagggatc gagcacattc atgatcttgt cgatcttccc cttgaaggtc
     1741 cggccggggt aggcgagggt ggtgacgtcg acattctccc cctgtttgat caggctgata
     1801 ttggattcgt atacgttagc ctggatccac accgtcttga gatcggagat ggtgaacagg
     1861 ctgttcgaat tatcggcgcg gatcgtcatg ttgttgatgg cggatttctg gacgatgaac
     1921 ccgccgatcg gggccttgat gacaaactcg ccctgggtgc cgccgccgtt gatctttagt
     1981 accgaatgca cccggttgag ttcggattgc gcctgctgga cggagatctc cgcgttcagg
     2041 gagtcggtca tggaagccag gccgcttttg aaaagatcct ttgtcttttg gagattggtc
     2101 tgcgccagac ggagattggt ctccgcattg acgaggtcgt tgctgtaacc ggccatctcg
     2161 gagctccgta caacgcccaa tacctggccc tgttgtacat aatcacccag catggctttt
     2221 acgtccgaga tattaccgct taccatactg tttaccggca cgacatgatc ttcatccgaa
     2281 cccaccttcc cggtaagggt gatggaattc accagttggg aaatggtgac ggtgtcgatc
     2341 ttcagactgc gcgcgatggt atcgggcaac acatattttt ccttgtcacc ggcaggtttt
     2401 tgctcctgcg agccgcaggc ctggaacagc agggcgctgc cggcaagaag gaagggaagg
     2461 aactggctac cggctttaaa gatcttttgc atattgatac gattgatcgt tggtgtattg
     2521 gttttagcgg gttgttgttt aattaaagaa attggttccc gtatagtaat tgatgtcctc
     2581 gaaggcgctg acccggttga acccgatgtt gttcagctgg agcgtgtttt gtttatacga
     2641 gtcgtagaaa tccaggaatt ccaggatgct gatattccgt ttctggtaat tggccagcat
     2701 ctcgtgcatc aggcgctgga aatccgcaaa gaatttcggg tcgatcgtct ggaagagctt
     2761 ttgctgcgca aaggcctttt gcagcgacgt ggcgacattc tcttccaccg tggcctgggt
     2821 gctgcgctgg gtcgcttccg tactggcgat ctgcagcttt gcattcttta tgttgccctg
     2881 gttatggttg aaaaaaggca ggtcgatgga ggcgccgatg ccgtagtagt tgtacaggaa
     2941 gctgcccgcc tcgtcatagc cgagggacag cgtaagatcg ggcaccgcca gggccttctg
     3001 gtaattgtag ttcaactggt ttatccgcgt gttggcacgg gcgatctgca gatcggtgcg
     3061 gttgtgatag gccgaatcga tgagcgtggt aagcgggtat ttagcggggt ccagctgggt
     3121 cagggccgcg gagtcgacct cggggtcgac aaagaaattg ggtttaacct ggagcaccag
     3181 ccgcagctcg ctttccgtac tgttgacctg gttgatcagg tcgctgtatt cgctctggaa
     3241 actatacagc tgcgccttta tccgcaccac ctctttctcg gaaatataac ccttgccctg
     3301 ttgttcggca aaagcgttga ctatctggct cagggccttt atctccgcgt catacacctt
     3361 tgcagactgt tgcagataat agatctggaa gaaatccgtg cgcagggtgt atttcagcgt
     3421 acgcagcaga tcgaagaact ggtattccgt caggtgaacg ttctcctggg caagttttac
     3481 ctgtttattg cgctttccgg caagcaggat caattgggat agccccaccg tggtctcatc
     3541 gttcctgccc aggggaaaga attcattcaa tgtcccggaa tacagggtat ggccgatgct
     3601 gaagtttgga ttgggccata gccgcgcctg aaggacaagg gcctggctgg catcgatgtt
     3661 gtaccgctgg gcaaggagct gcaggttgct gtccaggaac attttctctg ccgaggataa
     3721 ggataaacgg agcgtatcct gcctgggctg cgcagtcacc gcggcaccga taaaacaaag
     3781 cccggcaaaa agagatagcc actttctatg cataggtcta gcgtggaatt ttttcgtgtg
     3841 caaaagtatt ctattttaat gttaatcagg ggttaacccc tcgggtcccc caaatttggg
     3901 ccctttctca tatataagtg taatcgttta tttgtcccgg cctgcagaga cctggacagg
     3961 aacatatggc agcggtgaat ggaaaaacag gcaaaggaat gaaaaataac gcgcgcgggg
     4021 cctatttatt ggtaaacaaa ttattaaccc ccccccgctt aactacctgt taaccctgtg
     4081 gggtgttgaa acggaagccc acaccgtgga tggtctccag gtttacctgc ggatcggctt
     4141 taaaatattt cctcagcttg gtgatgaaca cgtccatgct tcgtcccagg aagtagtcgt
     4201 ccttgcccca tacgttcaac aacacctcct cccgcttcag gatgcggttg gaatgttcgc
     4261 aaaggaattt gagcagatcg gcctctttct gggtgaggtt gaacacttcc tggccgatgg
     4321 taagtttcag atcggtgtag gagaaattga gctgaccgat cgtaaaattg accgttctgt
     4381 ccgagtgcat cttccgggtc ctccggagga atacctccat ccgcatcagg agctcctgga
     4441 tactgaacgg ttttgtgata tagtcgtcgg cgccgctttg aaaccccttg atcttatctt
     4501 cttccatgga ccgggccgtc aggaaaagga tgggtaccag gtcgctttgc tgccggatct
     4561 tcctggcaac cgcgaatcca tccttgttcg gcatcatgac gtcgagcagg cagatgccga
     4621 attcgttctt atcgaacttc tcgatagctg cctgtccgtc aggacataat actacctcat
     4681 aaccggcatc taccaaattg tccttgatga catatccgag ggaaaggtcg tcttcagcca
     4741 gtaacacttt tacttttgac acttgttgca tagataaatc ttagttaagg ggaataaata
     4801 atctgaattc cgtaccgatg cccggcagac tgttgaccct gatcttgcct ttatgcgcgt
     4861 cgatgatccg tttgacgaaa ttgagaccca gcccaaagcc ttttacatca tggatatccc
     4921 cggtaggtac ccggtagaat ttcttaaaga tgttcttcag gtattgtttt tcgat
//
LOCUS       DATGFG010000769         4345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_14584, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000769 DATGFG010000000
VERSION     DATGFG010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14584"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VKQ52_14420"
     CDS             <1..633
                     /locus_tag="VKQ52_14420"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HLZ88446.1"
                     /translation="LDGALPGEMPGYNFARVGVATGDTVDGNYTYVNSFRPLGHESRD
                     IGQFIDDDGTAYLVFEDRPYGFRIARLAAADYMSLDKEMCLIPKHMEGGAIVRYGGLY
                     YAIGSALTGWRANPNKYATAASLEGPWSEFTDIAPPEKNTYGCQSTMLLKVAGSKDTT
                     VIFMGDIWKPGMQWESTYLWMPVEIGGGKLWVPEPRGWAIDVRRGSWAPR"
     gene            796..1233
                     /locus_tag="VKQ52_14425"
     CDS             796..1233
                     /locus_tag="VKQ52_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88447.1"
                     /translation="MKKLFFSVLLLPFAGRPGVVKSPSRPANLPENIVEVRSGAWPIN
                     LERNADGVGTYSLIYRNQEVMSGVSLDTLTFSDVGQLKYFAKGLEALRVGHNGDIANF
                     KEYSITRSDKKFEGVWYLLRYQFGSTSFQQPEADIMGKTIKGL"
     gene            1302..1751
                     /locus_tag="VKQ52_14430"
     CDS             1302..1751
                     /locus_tag="VKQ52_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88448.1"
                     /translation="MKKLLVLFFFAPFISKAQFTISNSSTTSIVELRTGTWPLDLQRI
                     IKNTDTCYALQFRDQQVTDDVNMATLRFGGLDQLRYFQKGLAALKTGANGDIANYKDY
                     TIKRMDVKKDLDPKKNGIWYMVTCNDGPVTNFQQTEADKMIAAIKTL"
     gene            complement(1832..3358)
                     /locus_tag="VKQ52_14435"
     CDS             complement(1832..3358)
                     /locus_tag="VKQ52_14435"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ88449.1"
                     /translation="MKNVTSSSIGRRQFLETAATAALGITILPRHVLGGRNYIAPSDR
                     ISIAYIGCGTQGIRELLPLLASPAFQVVAVVDPNRDAIGYRDWAKGWLLNEIRTAIKS
                     PNWEAGGDNNIPGGRENGKSIVETFYANVRPEGKYKGCHTYADYRELLDKEKDLDAVK
                     IMTPDHLHGVISMAAMKKGKHVMVHKPLSNRLLEGKKVIEYARNSKVITHLIPWDSNG
                     DMGPVMAMINNGDIGTLKEVHCWTNRPVWPQYPTLPTDTPAVPPGLDWDLWLGPEAQR
                     PYHPNYTNMVFRGWYDFGGGSMADMGHYALWTVYKALELEGPTMVIPHLSHVVGMNEP
                     VPFQIKNDFSFPMASTVRLKYPAKGSRPPVDLCWYDGGMRPPIPIELLEEDKELPAEG
                     QLFVGDKGKILTDFYVGNPVLLGKPAPNQAPNRVNQVERGAAALQKFVDAVKSGNQYP
                     GNFREAEGITEAINLYAVALRSGRMLKYDAAKRTITNVAEANKYLSREYRPGWDPFTA
                     "
     gene            complement(3548..>4345)
                     /locus_tag="VKQ52_14440"
     CDS             complement(3548..>4345)
                     /locus_tag="VKQ52_14440"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HLZ88450.1"
                     /translation="ATGHPSHQGVIILFDGNNGTPLMLFDAEEITAIRTAAASALATK
                     LLANEDASTLAIIGAGEQAERHIESIPLVRPIKTIRLWGRHREKAEQLARKANLLAAT
                     NITTNSTTSNSTNITSVDPNTPKTQTSIIITDTPQQAVTGADIICTVTAAGEPVVKGE
                     WLSPGAHLNVVGSCTPSAREVDTETILRSKLFTDRLESLFNEAGDFLIPKNEGAIAES
                     DVRGEIGEVLTGTKRGREHPTDITLFKSLGIAAEDLYSAWHLYKKLR"
BASE COUNT          763 a          875 c         1603 g         1104 t
ORIGIN      
        1 ttggatgggg cactgccggg ggagatgccg ggatacaatt ttgcgcgcgt gggcgtggcg
       61 actggtgaca cggtggatgg caattacacg tacgtgaaca gtttccggcc gctggggcac
      121 gagagccggg atatcgggca attcatcgat gatgacggga cggcctatct tgttttcgag
      181 gaccggccgt atgggtttcg gattgcgcgg ctggcggcgg cggattatat gagcctggac
      241 aaggagatgt gtcttattcc gaagcatatg gaagggggtg cgatcgtgcg gtatggggga
      301 ttgtattatg cgatcggttc ggcgctgacg ggatggaggg ctaaccccaa caagtatgcg
      361 acggctgcct cgttggaggg gccgtggtcg gagtttaccg atattgcgcc gccggagaag
      421 aacacgtatg ggtgtcagtc gacgatgttg ttgaaggtgg cggggtcgaa ggatacgacg
      481 gttattttta tgggggatat ctggaagccg gggatgcagt gggagtcgac gtatttgtgg
      541 atgccggtgg agattggcgg ggggaagttg tgggtgccgg agcccagggg atgggcgatc
      601 gatgtgagga gggggagttg ggcgccgcgt tagggattgg aggcagtacc gtgtatgccg
      661 gaaagcccga cccgccgccg gttgtcggca ttgtttgcgg cggcggggaa cgctatagat
      721 cgggatggat cggtgcatga tcggcatgat ttatgcagag aattggggtt taacctaaat
      781 ccgtccaatt gtaatatgaa aaagctattt ttcagtgttt tattgctgcc ctttgcgggt
      841 aggccgggtg ttgtcaagtc tccttcgcgg ccggcgaatt taccagaaaa tattgttgaa
      901 gtgcggtcgg gtgcgtggcc gatcaatctc gagcggaatg ccgatggggt tgggacgtat
      961 tcgctaatct atcggaacca ggaagttatg agtggggtga gtttggatac gctgacgttt
     1021 tccgacgtgg gtcagctgaa atattttgcg aaggggctgg aagccttgcg ggtagggcat
     1081 aatggggata ttgcgaattt taaagagtat tcgatcacgc ggagtgacaa gaagttcgag
     1141 ggggtttggt atttgttgcg gtaccagttt ggatcgacga gttttcaaca acccgaggcg
     1201 gatattatgg ggaaaactat caaaggtttg tagggttgcg tacatactag atcgcgaatt
     1261 cgtccgttaa atttagttat atcagaaaat tcatcaacgc tatgaaaaag ctccttgttt
     1321 tatttttctt tgcgcctttt atttccaaag cgcagtttac gatctcgaac agcagtacga
     1381 cgagtatcgt cgagttgcgg acgggcacct ggccgttgga tctgcagcgg atcatcaaga
     1441 acacggatac ctgttatgcc ttgcagtttc gcgatcagca ggtgactgat gatgtgaata
     1501 tggctacgct gcgatttggc ggccttgacc agctgcggta ttttcagaag ggtttggcgg
     1561 cgctgaagac cggcgcgaat ggagatattg cgaattacaa agattatacg atcaagcgga
     1621 tggatgtgaa gaaggatctg gatcccaaga agaatgggat ctggtatatg gtgacgtgca
     1681 atgatgggcc ggtgaccaat ttccagcaga ccgaggcgga taagatgatt gccgcgatca
     1741 agacgttata agatcgcctg ggcagacggc gtgatcggat gtttgcgacc gcgtcgggtg
     1801 tttgcggggt gatcgggcgt ttgtggccgg ttcaggccgt gaacgggtcc cacccggggc
     1861 ggtattccct tgacagatat ttgtttgctt ctgcgacgtt tgtgatggtg cgcttggcgg
     1921 catcgtattt tagcatgcgg ccggagcgga gggcgacggc gtagaggttg atcgcttctg
     1981 ttatgccttc ggcttcccgg aaatttccgg ggtattgatt tccacttttt acggcatcga
     2041 caaatttttg gagggcggcg gcgccgcgtt cgacctggtt gacgcgattg ggggcctggt
     2101 ttggtgcggg cttgccgagg aggaccgggt tgccgacata gaaatcggtg aggatctttc
     2161 ccttgtcgcc gacaaatagc tgtccttcgg cggggagttc cttatcttct tccagtaatt
     2221 ctatcgggat gggtgggcgc atgccgccgt cgtaccagca gaggtcgacc ggtgggcggg
     2281 aaccttttgc cgggtatttg agccgcacgg tgctggccat ggggaaggag aagtcatttt
     2341 tgatctggaa ggggacgggt tcgttcatgc ccacgacatg gctgaggtgg ggaatgacca
     2401 tggtggggcc ttcgagttcg agggctttgt agacggtcca gagggcgtag tggcccatgt
     2461 ctgccatcga tccgccccca aagtcatacc agccacggaa caccatgttc gtataattgg
     2521 gatgataggg gcgttgcgct tcggggccga gccagaggtc ccagtcgagg ccgggtggca
     2581 cggcaggggt gtcggtgggc agcgtggggt attgtggcca tacggggcgg tttgtccagc
     2641 aatggacctc tttgagggtg ccgatgtcgc cgttgttgat catcgccatg acggggccca
     2701 tatcaccatt ggagtcccag gggatgaggt gggtgatgac cttgctgttg cgggcgtatt
     2761 cgattacttt tttcccttcg agcaggcggt tggacagtgg tttgtgcacc atgacgtgtt
     2821 tgcctttttt catggcggcc atggagatga cgccatggag gtggtcgggg gtcatgatct
     2881 tgacggcgtc gaggtctttt tctttatcca gtaattcgcg gtagtctgcg taggtgtggc
     2941 agcctttgta tttgccttcg gggcgaacgt tggcgtagaa ggtctcgacg atggatttgc
     3001 cgttctcgcg gccgccgggg atgttgttgt cgccgccggc ttcccagttg gggcttttta
     3061 tggctgtccg tatttcgttg aggagccagc cttttgccca gtcgcggtag ccgatggcgt
     3121 cgcggttggg gtcgacgacg gcgacgacct ggaaggcggg agaggcgagg aggggcagga
     3181 gttcgcggat gccctgggtg ccgcagccga tataggcgat ggagatcctg tcgcttgggg
     3241 cgatgtagtt cctgccgccg aggacgtgtc tggggaggat ggtgatgccg agggcggcgg
     3301 tggcggctgt ttcgaggaat tggcgccggc cgatggagga ggaggtgaca tttttcatga
     3361 cagcaagttt atcgtttagt gaggaaacta aatgtagcga tttttttggt ttgttggtga
     3421 tgggggtgtt tggggcggca gtttgtggtg gcggtggcgg tttgcggcga cgggtgttgg
     3481 ggattttgac ggcagtttgt ggatggcagt ttgtggtggc ggcgacggtt tgcgggtggc
     3541 ctggaggtta gcggagtttt ttgtagagat gccaggcgga gtagaggtct tcggcggcga
     3601 tgccaaggga tttgaagagg gtgatgtcgg tggggtgttc gcggccgcgt tttgttccgg
     3661 tgaggacttc gcctatttcg ccgcggacgt cgctttcggc gatggcgcct tcgtttttgg
     3721 ggatgaggaa gtcgccggct tcgttgaaga gtgactcgag gcgatcggtg aaaagttttg
     3781 agcgcagaat ggtttcggtg tcgacctccc ttgccgatgg ggtacaggag ccgacgacgt
     3841 tgagatgggc gccgggggag agccattccc cttttaccac gggctcgccg gcggcggtga
     3901 cggtgcagat gatgtcggcg ccggtcacgg cctgttgtgg ggtatccgtt atgatgatgg
     3961 aggtttgtgt tttgggggtg tttgggtcga cggaggtgat gttggtggag ttgctagtgg
     4021 tggagttggt ggtgatgtta gtggcggcga gaaggttggc ctttcgggcg agttgttcgg
     4081 ccttttcccg gtggcggccc cagagccgga tggtttttat ggggcggacg aggggaatgg
     4141 attcgatgtg gcgttcggcc tgttcgccgg cgccgatgat ggcgagggtg gatgcgtctt
     4201 cgttggccag gagttttgtg gcgagggcgc tggcggctgc ggtgcggatg gcggtgatct
     4261 cttccgcgtc gaagagcatg aggggtgtgc cgttgtttcc gtcgaagagg atgatgacgc
     4321 cctggtggga ggggtggccg gtggc
//
LOCUS       DATGFG010000770         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_835450, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000770 DATGFG010000000
VERSION     DATGFG010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835450"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2471)
                     /gene="sprA"
                     /locus_tag="VKQ52_14445"
     CDS             complement(<1..>2471)
                     /gene="sprA"
                     /locus_tag="VKQ52_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HLZ88451.1"
                     /translation="FPLTSWTLASTPAVPPNATSTYPALFPEANLNDSLDYGFNRAKL
                     AWYNIEPTLQDASNSNNPVAGYENPLADPRIGPIQVQQLFPQQSVQTGQAQLVTFDMA
                     YYPSDRGPYNFDARPGSINANGKLNNPQKRWGGIMRALDQTDFETANIEVLQFWVLDP
                     FAHPNNKPGNTTGGQLYIDLGSVSEDILKDGKKEFENGLNTPNITAQVDSSSVWGRVP
                     ANPIQVTTAFSNNAADRPYQDVGLDGMDNPAEQIKFARYLRSLSATLSPAAYQNANAD
                     PSSDDFTNYRDASYDNSKTGILGRYKNINNPQGNSPIASTGSTTISAYTLYPDQEDLN
                     KDNTMNTLEQYFEYKVNLTPDSLGKSNGYSFITDTVQFIPSGGVLQTWYQMTIPLAEY
                     YQNVGNIPDFRSIQFIRMYMTNWSDSAVLRFAQLQLVRNSWRSFSYVIDTTGNMTLLP
                     NSTTSFNVTAVNIEQNNSRQPINYVSPPGVVRQQELSSNNVNLLLNEQAMSLQICNLK
                     QGDVRGVYKTTNFDLRRYGTLDMFVHAEAVVQGSVNDFDMSYVVRLGSDFVANYYEIR
                     VPIHVTQWYAKAPTDIWPSANNLSLALSRLIQLKVDRNNKGTTNTYYKETDANGRSYA
                     IMGNPNLGAIQAVFLGVQNVNRSTVCTEVWFNELRLTDISEKGGWAAVGKVDVKLADL
                     GTLTVSGSNKSAGWGTIDQSTNQRSLENDAEIDAAINIDAGKLLPKKAAVSIPVYAGL
                     TKTTSTPEYDPFDLDIKLKDKINGAPAAQKDSIRKQAVDATTVETVNFTNVHKNNTSN
                     KKLKIWSIENVNVSYSYSRSEHHSPL"
BASE COUNT          446 a          616 c          767 g          642 t
ORIGIN      
        1 agcgggctat gatgctcgct ccggctatag gagtagctga cattcacgtt ctcgatgctc
       61 cagatcttta gctttttgtt ggaggtattg ttcttgtgga cattggtgaa attcacggtc
      121 tccaccgtcg tagcgtccac cgcctgtttc cggatcgagt ccttttgggc ggcgggtgcc
      181 ccattgatct tgtccttcaa tttgatatcc agatcgaagg gatcgtattc cggcgtgctc
      241 gtcgtctttg tcagaccggc gtatacggga atggacacag cggctttttt gggcaggagt
      301 ttcccggcgt ctatattgat agcggcgtct atctccgcat cgttctccaa tgatcgctgg
      361 ttggtgcttt gatcgatggt tccccatccg gcagacttat tactacccga tacggtaagc
      421 gtaccgagat cggccagttt cacgtcaacc ttgccgaccg cggcccagcc ccccttctcg
      481 ctgatatctg tgagccgcag ctcgttaaac cagacttccg tgcacaccgt gctccggttc
      541 acattttgga caccgaggaa gactgcctgg atcgcgccca ggttgggatt acccatgatg
      601 gcataggatc tgccgttcgc gtcggtctct ttgtagtagg tgtttgtagt gcccttgtta
      661 ttcctgtcca cctttaactg gatgagccgg ctaagggcca gactcaggtt gttggcagag
      721 ggccagatgt cggtcggcgc cttggcgtac cactgggtta cgtgaatggg cacgcggatc
      781 tcgtaatagt tggcgacgaa gtcactaccc aggcgaacga catacgacat gtcgaagtcg
      841 ttcacggagc cctggaccac ggcttccgcg tgtacgaaca tgtccagggt cccataccgg
      901 cggaggtcga aattggtagt cttgtatacg ccccggacat cgccctgttt caggttgcag
      961 atctgcaggc tcatggcctg ttcgttaagc aggaggttga cattgttgct gctcagttcc
     1021 tgctggcgga cgacgccggg cggagaaaca tagttgatcg gctgacggga gttgttctgc
     1081 tcgatattta cggccgttac attgaagctg gtagtgctgt tcggcagcag ggtcatgttc
     1141 cctgtcgtat cgatgacata ggaaaaactc ctccaggaat tgcgcaccag ttgtagttgg
     1201 gcaaaccgaa ggaccgccga gtccgaccag ttggtcatgt acatccggat gaactggatg
     1261 gaccggaagt cggggatgtt acccacgttc tggtaatatt ccgccagcgg gatggtcatc
     1321 tggtaccagg tctgcagtac ccctccggag gggatgaatt gcaccgtgtc ggtgataaat
     1381 gaatacccgt tgcttttccc aaggctgtcc ggtgtaagat tgaccttgta ttcgaagtat
     1441 tgctccaggg tgttcatcgt attgtccttg ttcaggtctt cctggtcggg atacaacgtg
     1501 taggcggaga tggtcgtcga acccgtggag gcgatgggtg agttcccctg tgggttgttg
     1561 atgttcttat aacggcctag tatacctgtt ttcgaattgt cgtacgaggc atcccggtaa
     1621 ttggtaaagt cgtcgctgga agggtcggcg ttggcgttct ggtaggcggc cggggacagg
     1681 gtcgccgaca gcgacctcag gtagcgcgcg aattttatct gttcagcggg gttatccatg
     1741 ccgtcgagac ccacgtcctg gtaggggcgg tctgcggcgt tattgctgaa ggcggtggtt
     1801 acctggatgg gattcgccgg caccctgccc cagacggaac tgctgtctac ctgggccgtg
     1861 atattcgggg tattgaggcc gttctcgaat tcttttttac cgtccttcag gatgtcttcc
     1921 gagacgctgc cgagatcgat atagagctgg ccgccggtgg tgttaccggg tttgttgttc
     1981 gggtgtgcga aggggtccag cacccagaac tgcaggacct cgatattggc cgtttcgaag
     2041 tcggtctggt cgagggcacg catgatcccg ccccagcgtt tttgtgggtt gttcagtttg
     2101 ccgttggcgt tgatgctccc cggccgggca tcgaagttgt atggacccct gtcgcttgga
     2161 tagtaggcca tatcgaaagt gaccaactgg gcctgaccgg tttgtacgga ctgttgagga
     2221 aacagctgtt gtacctgtat cgggccgata cgcgggtcgg ccagggggtt ctcgtaaccc
     2281 gccacggggt tgttcgaatt gctggcgtcc tgtaaggtag gctcgatatt ataccaggcc
     2341 agttttgcgc ggttaaagcc atagtccagc gagtcattga gattcgcttc ggggaagagg
     2401 gcgggatacg tgcttgtcgc gttgggcggg acggccgggg tagaggcaag ggtccaggat
     2461 gtcagcggga a
//
LOCUS       DATGFG010000771         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1614160, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000771 DATGFG010000000
VERSION     DATGFG010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614160"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2492
                     /locus_tag="VKQ52_14450"
     CDS             <1..>2492
                     /locus_tag="VKQ52_14450"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88452.1"
                     /translation="TVKPNQTAIVDLIYGITETREACEGLMRKYRDEHLKNRAFELSW
                     THNQVLLRQINATEADAQLYDQLASSIVYPNPDLRGEAAVLLNNFRGQSGLWSHSVSG
                     DLPIVLLHMADPDNLDLARQMIQAHAYWRLKGLSVDLVIWNEDHGSYRQLLQDQLQGL
                     INTDAGNHSYQKPGKIFVRSADQISPEDRILFESVARVIIYDHKGTLSEQVNRIHTEK
                     VVLPFVPTGPQAAEESPGNTTLPDNLLFDNGTGGFTPDGKEYKILTDRKKTTPAPWVN
                     VIANPDMGTVISESGSSYTWAINAHEYRLTPWNNDPVSDAGGEAFYLRDEESGYFWSP
                     VPFPVRGTTPYIATHGFGYSLFQHTEQGISTELCVFIDQALPIKWMVLRVKNQSGRRR
                     RLSATGYLEIVLGDVPAKTNMHILSEQDADSGALLFRNRYNTAFSDRVTFFKVDDPSQ
                     LSFTTDRLEFIGRNRNLQDPQAMHRKRLAGRSGAGMDPCAALQVVFDLADNAEKEIVF
                     LLGSATNTREAEELIRKGSGPQAASLSLLHVKKHWGTILGAVQLTTPDDALNIFANGW
                     LLYQTLSCRLFGRSGFYQSGGAFGFRDQLQDVLAILHTRPDLAREQILLHASRQFAEG
                     DVQHWWHPPEGRGVRTRCSDDYLWLPFVVARYVAVTGDGDLLATTIDYLEGRPLYQGE
                     DSFYDLPVTGNLNGTLYEHCVRAIRHGLRFGIHGLPLMGTGDWNDGMDKVGYKGEGES
                     VWLAFFLYDVLMRFADTADTFKDHFFAEICRTEAAGMQPRIETAGWDGGWYRRAYFDD
                     GTPLGSKENKECRIDAISQSWAVLSGSADTGR"
BASE COUNT          562 a          720 c          688 g          522 t
ORIGIN      
        1 ttacggttaa gccaaaccaa accgcaatcg tcgatctgat ctatgggatc actgaaacaa
       61 gagaagcctg tgaaggcctg atgcgtaaat accgggacga acacctgaaa aaccgcgcct
      121 tcgagctctc gtggacccac aaccaggtgt tgctgcgcca gatcaatgcg acggaggccg
      181 atgcccagct ttatgaccag ctggcatcct ccatcgtcta tcccaatccc gatctgcgtg
      241 gagaagcagc cgtgctcctg aacaatttca ggggacagtc gggcctttgg agtcactcgg
      301 tgtccggaga cctcccgatc gtattgcttc atatggccga tccggacaac ctggaccttg
      361 cccggcaaat gatccaggcg catgcctact ggcgactgaa ggggctttct gtcgatctcg
      421 tcatttggaa tgaagatcat ggttcttacc ggcaactgct gcaggaccag ctccagggcc
      481 ttatcaatac cgacgcaggc aaccactcct atcaaaagcc cggcaaaata tttgtacggt
      541 ccgcggatca gatctcgccc gaagaccgta ttctgtttga aagcgtcgcc agggtcatca
      601 tttatgacca taaagggaca ttgtccgaac aggtgaaccg catccacacc gaaaaagtcg
      661 ttttgccttt tgtgccgacg ggtccccagg cggcggaaga atcacccggc aacactacgc
      721 tgccggacaa ccttctcttt gacaatggca ccggcggctt caccccggat ggtaaggagt
      781 acaagatcct caccgacaga aagaagacga cccctgcgcc atgggtcaat gtgatcgcca
      841 accctgatat gggcaccgtc atttccgaaa gcggctcttc ctacacctgg gccatcaatg
      901 cccatgaata caggctcaca ccctggaaca acgatcccgt aagcgatgcc ggcggagaag
      961 ccttttatct gcgggacgag gaatccggtt atttctggtc tcccgtgccc ttccccgttc
     1021 gcgggaccac accgtatatt gcgacgcacg gatttggtta tagtcttttt cagcataccg
     1081 agcaggggat ctccactgaa ctgtgcgtgt ttatagacca ggcgctgccc atcaaatgga
     1141 tggtattgcg ggtaaaaaat caatcgggcc gaagacgcag gctttccgcc accggctacc
     1201 tcgagatcgt gctgggagat gttcccgcaa agacaaacat gcatatcctt tccgaacagg
     1261 atgcagacag cggcgccctg cttttcagga accggtacaa cacggcattt tccgatcggg
     1321 tgaccttttt caaggtggac gatcccagcc aattgagctt caccaccgac agactcgaat
     1381 tcatcgggcg gaacaggaac ctgcaagacc cgcaagccat gcaccgcaaa aggcttgccg
     1441 gcagaagcgg cgccggaatg gacccctgtg ccgccctgca ggtggtgttc gatctggccg
     1501 ataacgcgga aaaggaaatt gtattcctgc tgggaagcgc cacgaacacc cgggaggcgg
     1561 aagaactcat ccggaaaggg tccggccccc aggcggcatc gctctcactc ctccacgtaa
     1621 agaaacactg gggaacgatt ttgggtgccg tacagctcac cacccccgat gacgccctga
     1681 atatcttcgc caatggctgg ctgctttatc agaccctatc ctgccggctc tttggccgga
     1741 gcggttttta ccagtccggc ggggcctttg gattcaggga tcagctccag gacgtgctgg
     1801 ccatccttca tacccggccc gacctggccc gcgaacagat cctgttgcac gcctccaggc
     1861 aatttgcgga aggcgatgta caacactggt ggcatccccc tgaaggcagg ggcgtcagaa
     1921 cccgttgttc cgatgactac ctttggctgc cctttgttgt tgccaggtat gttgccgtca
     1981 ccggtgatgg tgacttgttg gcgacgacca tcgactacct ggaaggccgc cccttatacc
     2041 agggagaaga ttctttctac gatctgccgg tgaccggcaa tctgaatgga acgctctatg
     2101 agcactgcgt ccgggccatc cggcacggct tgcgttttgg catccacggt ctgccgctga
     2161 tgggtacggg cgactggaat gacggaatgg acaaggtggg ttacaaaggc gaaggagaaa
     2221 gtgtctggct tgcttttttc ctctatgatg tcctgatgcg atttgccgat accgcggata
     2281 cattcaaaga ccatttcttc gccgagatct gtcgtacgga agcagccggt atgcagccca
     2341 ggatcgaaac ggccggctgg gatggcgggt ggtacaggcg ggcttatttc gacgatggaa
     2401 ccccgttggg gtcgaaggaa aataaagaat gccggatcga tgccatctcc cagagctggg
     2461 ccgtactgtc cgggtcggct gacaccggtc gg
//
LOCUS       DATGFG010000772        10401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_869725, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000772 DATGFG010000000
VERSION     DATGFG010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10401
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869725"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VKQ52_14455"
     CDS             complement(<1..1234)
                     /locus_tag="VKQ52_14455"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88453.1"
                     /translation="MNNKLYTAALISTLCLLLAACTKNLNRLPTNAITAAQVYTTPLG
                     YTQALSKAYSAFANTGNKGGTGSPDISTQIISDEGNSDFLRLYFNLQELTTDEAAWTW
                     ESDAGIQGLHEMSWSSINPIVDGLYYRSFFQITICNDFISHSTDAALAGKGFSSQTVD
                     SIRHYRAEARFLRAYQYWVLMDLFANPPFATENDAIGTFIPKQISRSDLFKYIESELK
                     GAADSGLLAPRKALYGHADQAAAWALLARLYLNAQVYTGTARYTDAITYCNKVIGAGY
                     TLHPDYTKLMLADNNLNTDENIFTIVYDATYIQNYGGTTYLVHGPAGIPDSVSGCNGS
                     WSGLRLTQNFISLFPDNSGSTDHRAQFWTPGQTEAMTALYTSTAGFSTFKFRNLTHTG
                     APDPDRDPAGNFASIDFPL"
     gene            complement(1264..4302)
                     /locus_tag="VKQ52_14460"
     CDS             complement(1264..4302)
                     /locus_tag="VKQ52_14460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ88454.1"
                     /translation="MKIRLLARMHILILLLCLCSALSAQKRTITGKVTDAKDGSPLAG
                     VSVQPKGDLKSGVVTGNDGGYTLSVDASVKTLVFSIIGFGTLERPIQGGSLDIALTAG
                     ASGLNEIVVIGYGTARKKDLTGAVAVVDEKEFQKGTITTPEQMIAGKVPGVSIISNSG
                     QPGAGSTIRFRGGSSLNASNNPLIVLDGVPLNNDVAPNQKASIPGSSSPLSFINPNDI
                     ESFTVLKDASATAIYGARAAGGVIIITTKKGRGGALKVNLHSNNSISTLIKQVPVLTA
                     AQFRSVVTTNGTAAQVAAMGSSNTNWQDQIYQTAVGTDNHISLSGGVKDLPYRLSIGY
                     QDQNGILKTDNLQRTSASLALNPVLFDKHLKVNLNLQGSYESARFGNQAAIGGAITFD
                     PTQPIYTKSPRFGGYYEWLDPTTATGLKALVGRNPLGLLEQHFDRATPQRSIGNLQLD
                     YSFHFLPDLHANLNAGYDLSNGKGTTFVPDSAAESYVAGGVNSGQNNKYKLTNANTLF
                     EFYLSYSRNLKKIKSRFDVLAGYSYNNYLSTVYNYPSFYANGTQVPASQPATPSAQYE
                     HTLISYFGRANYSFEDKYLLTGTIRRDGSSRFAPQNRYAVFPSAAFAWKIKSESFLSN
                     VDVVSELKLRLGYGLTGQQDGIGDYDYLAYYYLSDSNAAYQFGNNYYQGYRPAGFYAA
                     RRWEQTATSNIGLDYGFLDNRITGAIDFYYKKTTNLLNTVPQPAGSNFSAYITANVGT
                     MTNKGVEFSVNFQPVRTRDLTWDIGFNVTYNKNTITNLTVIPGDTNYTGFGSGNIAGG
                     IGGQFAEINAVGSPKNTFYLYRQVYDVKGHPIEGVFQDKNGDGIINQQDLYKTKPADP
                     TTFMGFTTNVTWRRWNAGCVLRASLGNYAYNNIFSQTGNLNQMIHNVTLYNASSSYLT
                     THFTGGNAQQLLSDYYISNASFLRMDNITIGYNFGAISHTHTNLRLNAGVQNVFVITK
                     YTGLDPEIGAGTDVGLGNPGIDNNLYPRPRVYNLGVNLDF"
     gene            4506..8555
                     /locus_tag="VKQ52_14465"
     CDS             4506..8555
                     /locus_tag="VKQ52_14465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ88455.1"
                     /translation="MGAARAQGPKLRFTHLSGEQGLSNSTIEAIYQDSQGFIWIGTRD
                     GLNRYDGHEIVVYRNDPADSGSISDGYIRCILEDRDHQLWIGTVNGLNRFDRAKDRFT
                     RWKHRDADSGSLSSSNITSLLEDHRGHIWVATSGGMNLLDKKTGSFRHFRMGGGAGSL
                     RDDRINCLQEDKSGDGTIWVGTQSGLDIFHSETGLFTAVANPEITNPSGNTIIAIRED
                     RQGNLWLGTEDDGLYLFDPLRKSFTRYGHSDTDASSLGNNMIKCMLTDHKGQLWAGSI
                     NGGLNLFHASSGSFFHYAYEPGNGSSLSQRTISALFEDRQGNLWVGTHRGGINIYSPG
                     LEKFNLYRQEASPNSLSYNDVKTFCEDRGGNIWVGTDGGGLDLFLRDERVFRHYRNDP
                     FNDRSLGSNAVLDVFQDREGELWVSTWGGGLNHFDRQRGDFTRYQNNPADRYSITSNF
                     VQKTFEDFSGNLWVATYYGGLNIFDRKKKQFTRLIDDPTGKTSLTGKNIVSLLEDREG
                     RIWIGTDDGGLNCWNENTRHFTHYFDQEEKMPDLRVLFCDSHGRLWVGQKGLYLFDAA
                     RDSFRLYANRSGLSNEFIKGITEDGQGNLWISTSNGLTQLNADGYGFKKYNTGDGLQD
                     LEFEANAFLKTRDGEMYFGGINGFNSFYPGAIMANAFVPPVYITGFQLSNRKIGPGNG
                     SPLDRDISMMREIRLSYRQSTFSFTYSALNYTTPENNQYAYKLEGLDTAWNYVGKETK
                     AVYTNLMPGDYVFRVKASNNDGVWNEDGATVKVIITPPFWRTGWFISLLVALGIAGLY
                     VWYKFRTRLKMRELEEKKREEMRQVQLQFFTNVSHEFRTPLSLILGPLEKMMKEYNTP
                     VLNRYFQAMHRNAQRMLSLIHELMDFGKLESGSLRLCVQAGSLNTFMEELAEEFRDWA
                     VQKEIDFCLEGGLTGEAWFDRQVLEKIVLNLLQNSFKYTKAGGRITLQLLGSMEGFTP
                     SYAGELVLKNPNRGSRYAYIRVADTGIGISAGSIHHLFERYYRITESHLGSGVGLAFV
                     KSLALLHKGDIYVYSERLKGTEIIIGIPISVDDYGVEEKRAIHVEGGVRLEGFPVTRG
                     EAPAVNLPPVETILLVEDNEELRHFLKDSLSPYYQVMEAADGREGLDKAREIAPGMII
                     SDVIMPGMNGFDLCRAVKQDIDICHIPFLMLTARSAAAAQLEGLDAGADYYFAKPMNM
                     ELLLLTIRNRFDQDRKLKERYSRESQVEAMELVHSEKDREFMRRLLNTIDSQLSNPDF
                     DIDWLCQEMGMSRTKLYQKIKGISQQSIGDFIRTIRLKKAVQIMTHEDVTLTEVMMRI
                     GIQTQSYFTKAFKKEFGKTPTQFMQEIRK"
     gene            8677..>10401
                     /locus_tag="VKQ52_14470"
     CDS             8677..>10401
                     /locus_tag="VKQ52_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88456.1"
                     /translation="MLKNYFKTALRNLWKQKGFTAINLTGLALGMACSLLILLWVQDE
                     RSVDGFHVKGDRLFYVYERNHMGARLQSWYWTQGPLAEELKKDIPEVEASTVIASKYP
                     AVFGVGDKKLKETGFAAQPDFFSMFSYPLLQGKAADALNSPDGIAISQRMAIEFFGSA
                     EAAIGKAMRYNDKKVLMVKAVYADFSPRVDYRGDFILSWSGYIEDGNDWAKNWPSVDP
                     STCVLLRTGASPAAVEAKMRHLLDKFPTEQKDMKIDLGLQRFGDRYLHSDFNAAGVPT
                     GGRIEYVRLFGIIALFILVIACINFMNLTTARSVKRAKEIGVRKVMGAVRGLLIRQFI
                     GEAVLMAFLSLLLALMIVSLVLPAFNQLTGKSIALPMGDPGFWASLGGITLLTGLLSG
                     SYPAFYLSGFNPIRVLKAALPSGTRGDALFRQGLVVFQFALSIVLILATILITQQIHY
                     MQNARLGYDRENLVYLPIEGELGKKLEVLQAEAKQLPGIANLTLYSGDNPTVMGTGTL
                     GMRWTGKDPNEHDRWIHEAIGRDFLSSMKIELVAGRDFSPDYPTDSNGVIVNETAVRL
                     MGYKDPIGK"
BASE COUNT         2190 a         2441 c         3408 g         2362 t
ORIGIN      
        1 agagcgggaa gtcgatgctc gcaaagttgc ccgcgggatc ccggtccggg tccggagcac
       61 cggtgtgggt caggttacgg aatttgaagg tggagaagcc ggccgtcgaa gtatacaagg
      121 cggtcatcgc ctccgtctga ccaggagtcc agaactgggc ccgatgatcg gtggaaccgc
      181 tattgtcggg aaagaggctg atgaagtttt gcgtgagccg caggccgctc cagctgccgt
      241 tgcaccccga gaccgagtcg gggatgccgg cggggccatg taccagatag gtggtgcccc
      301 catagttctg gatataggtg gcgtcataga cgatggtgaa gatattctcg tccgtgttga
      361 ggttgttatc ggcaagcatc agcttcgtat aatccggatg gagcgtatag cccgcgccga
      421 tgaccttatt gcaataggtg atagcatcgg tatagcgggc cgtgccggta tagacctgcg
      481 cgttgaggta cagacgcgcc agcagcgccc acgcagccgc ctggtcggcg tggccgtaca
      541 aggcctttcg cggcgcaagc agtccactgt cggcggctcc cttcagttcg gactcgatgt
      601 acttgaacag atcgctgcga ctgatctgtt tggggataaa ggtcccgatc gcgtcatttt
      661 ccgtggcgaa aggcgggttg gcgaagagat ccatcaatac ccaatattga taagcgcgaa
      721 ggaagcgggc ttccgcgcga tagtgccgga tggagtctac cgtctggctg ctgaagcctt
      781 ttcccgccag ggcggcatcg gtcgaatgcg agatgaaatc gttgcagatg gtgatctgga
      841 agaagctgcg gtagtagagc ccatcgacga tggggttgat ggaagaccag ctcatctcat
      901 ggagaccctg gatgccggcg tcactttccc atgtccaggc ggcttcatct gttgtcagct
      961 cctggaggtt gaaatagagc cggagaaagt cggagttgcc ttcatcggaa atgatctggg
     1021 tggaaatatc ggggctgccg gtaccaccct tgttaccggt gttcgcaaag gcggaatagg
     1081 cctttgatag cgcctgcgta tagccaagcg gggtagtata gacctgggcc gcggtgatag
     1141 cattggtcgg caggcggttc aggtttttgg tacaagccgc caaaagaaga cagagcgtgc
     1201 tgatgagggc ggcggtatat agcttgttgt tcatactttt cattttaaga tgtggtggtc
     1261 gggttaaaaa tccaggttga cgcccaggtt atagaccctt ggccgggggt aaagattgtt
     1321 gtcaattccc ggattaccga gaccaacgtc cgtaccggcg ccgatctccg gatcgagacc
     1381 ggtgtatttg gtgatgacga acacattctg aacgccggca ttcaggcgca ggttggtatg
     1441 ggtatgggaa atggcgccga aattgtatcc gatggtgata ttgtccatcc gcaggaaaga
     1501 ggcgttcgag atatagtagt cgctcagcag ctgctgggca ttacccccgg tgaaatgggt
     1561 agtgaggtag ctggatgagg cattgtaaag ggtgacattg tgaatcatct ggttgagatt
     1621 gccggtctgg ctgaagatat tgttataggc atagttgccg aggctcgctc gcaggacgca
     1681 accggcattc catcgacgcc aggtgacgtt ggtggtgaag cccatgaagg tagtcggatc
     1741 ggccggcttt gtcttgtaga gatcctgctg gttgatgatg ccgtcgccgt tcttgtcctg
     1801 gaacacccct tcgatgggat ggcctttgac gtcatacacc tgtctgtaga gatagaaggt
     1861 attcttgggg gatccgacgg cattgatctc ggcgaactga ccgccgatgc cgccggcgat
     1921 attgccgctg ccgaaaccgg tataattggt gtcgccgggt atgacggtca gattagtaat
     1981 ggtgttcttg ttataggtga cgttgaaccc gatgtcccag gtcaggtcgc gggtgcgtac
     2041 aggttggaaa ttgacgctga actccacgcc cttattggtc atggttccca cattggccgt
     2101 gatataggcg gagaaattgc tgccggcggg ctgtggaacg gtattgagga gattggtggt
     2161 tttcttgtag tagaagtcga tggcgccggt gatccggttg tcgaggaagc catagtcgag
     2221 cccgatgttg gaggtagccg tctgttccca tctgcgggcg gcatagaagc cggcaggccg
     2281 gtagccctgg taatagttgt tcccgaactg gtaggcggca ttggagtcgc tgaggtaata
     2341 ataagcgagg taatcataat ccccgatgcc gtcctgctgg ccggtgagcc catagcccag
     2401 ccgcagcttg agttcggata cgacatccac gttgcttaga aaagattccg attttatttt
     2461 ccaggcgaac gcagccgaag ggaagacggc gtagcggttc tggggtgcga aacgggatga
     2521 gccgtcccgg cggatagtac cggtgagcag gtacttatct tcgaaactat agttggcccg
     2581 gccgaaataa gagataaggg tatgctcata ctgggcagac ggagtggcgg gctgggaagc
     2641 gggcacctgg gtcccgttgg cgtaaaaaga gggataattg tagaccgtgg atagatagtt
     2701 attataggaa taccccgcca ggacgtcgaa acggctcttg atctttttga ggtttctgct
     2761 atagctgagg tagaattcga aaagggtatt ggcgttggtg agcttgtact tgttgttctg
     2821 accgctgttg acgccgccgg cgacatagct ctcggcagcg ctgtcgggca cgaaggtagt
     2881 ccccttgcca ttggagagat catagccggc attgaggttg gcatgcaggt cgggcaggaa
     2941 atgaaaggaa tagtccagct gcaggttacc gatgcttcgt tgcggtgtcg cgcggtcgaa
     3001 gtgctgttcc agcaggccca gggggttgcg gccgaccagc gctttgaggc ccgtagccgt
     3061 ggtcgggtcc agccattcat agtatccgcc gaaacggggg cttttggtat agatgggctg
     3121 ggttggatca aaggtgatgg cgcccccgat ggccgcctga ttaccgaaac gtgcgctctc
     3181 gtaggagccc tgcaggttga ggttcacctt aagatgtttg tcgaagagga cggggttgag
     3241 ggccagggaa gcggacgtac gctgcaggtt atccgttttc aggatgccat tctggtcctg
     3301 gtagccgatc gagagccggt aaggcagatc cttcacccca ccgctaagac tgatatggtt
     3361 gtccgtgccc accgcggtct ggtagatctg gtcctgccaa ttggtattgg acgaccccat
     3421 cgccgcgacc tgggccgcgg tgccgttggt ggtgacgacg gaacgaaact gggctgccgt
     3481 aagcaccggc acctgtttta taagcgtgga aatggagtta ttggagtgca ggttgacctt
     3541 cagcgcacct cccctgccct tttttgtggt gatgatgatg accccgccgg cagcgcgggc
     3601 gccgtaaatg gccgtcgccg aggcgtcctt gagaacagtg aaggattcga tatcattggg
     3661 gttgataaag ctgagcgggc tgctggagcc ggggatcgaa gccttctggt tgggggcgac
     3721 atcgttgtta agaggtacgc catcgagaac gatgagggga ttgttactgg cattgaggga
     3781 ggagccgccc cggaaacgga tcgtactgcc ggcgccgggc tgaccgctgt tggaaatgat
     3841 ggacacgccg gggacctttc cggcgatcat ctgttcggga gtggtgatcg tgcctttctg
     3901 aaattccttt tcgtcgacta cggcgaccgc gccggtcagg tcttttttac gggctgtgcc
     3961 atacccgatg acgacgatct cattcagacc ggaggcgccg gcggtgaggg cgatgtccag
     4021 gctgccgccc tggatggggc gttccagcgt tccaaagccg atgatggaaa agaccagggt
     4081 ttttacactg gcgtcgacgg agagggtgta gccgccgtcg ttgccggtga ctacgccgct
     4141 tttaaggtcg ccttttggct gaacggagac tccggcgagg ggggagccgt cttttgcgtc
     4201 cgtcaccttg cccgtgatgg ttcgcttttg ggcggaaagg gcagagcaga ggcataggag
     4261 taaaataagg atgtgcatcc ttgcaagcaa tcgtattttc atatcgttgg ttttgaccgg
     4321 gatacaaggt aggtggtgtt ggggagagcc ttttgttcta acaggtattt ctctggttct
     4381 atccgttcat tcctggtttt acgccgcttt tgccggtgaa aaagtggagg gagggggtta
     4441 catttgagta aagaatattg ccggccatgt ggaggatact tataataata ggtgtatttg
     4501 ccgggatggg tgcagcccgg gcccagggac cgaaactgcg ctttacccat ttgtcggggg
     4561 agcaggggtt atccaacagc accatcgagg ccatctacca ggacagtcag ggatttatct
     4621 ggatcgggac ccgggacggg ctgaaccgct atgacggtca tgagatcgtc gtataccgga
     4681 atgaccccgc cgacagcggg agtatcagcg acgggtatat ccgttgtatc ctggaagacc
     4741 gggatcatca gctctggatc ggaacggtga atgggctcaa ccggtttgac cgggcaaagg
     4801 accggtttac ccggtggaag caccgggatg ccgacagcgg cagcctgagc agcagcaata
     4861 tcacctccct gctggaggat catcgcggac atatctgggt tgcgacctcc gggggcatga
     4921 atcttctcga taaaaagacg ggcagcttcc ggcatttccg gatgggcggc ggtgcagggt
     4981 cgctgcggga cgaccggatc aattgtctgc aggaggataa gagcggggat ggaactatat
     5041 gggttggtac gcagtcgggg ctcgacattt tccattcgga aacggggctg ttcacggccg
     5101 tggccaaccc cgagatcaca aaccccagcg gcaataccat catcgccatc cgggaggacc
     5161 ggcagggcaa tctctggttg gggacagagg acgacgggct ttatcttttc gacccgctgc
     5221 ggaagtcctt cacccgatat ggtcattccg acacggatgc atcgagtttg gggaataata
     5281 tgatcaaatg tatgcttacc gaccacaagg ggcagctctg ggcggggagc atcaacgggg
     5341 gactcaatct ttttcatgct tcttcgggtt ccttctttca ttatgcctac gagcccggta
     5401 acgggagcag tctttcccag cggaccatct cggcgctgtt cgaggaccgc cagggcaacc
     5461 tctgggtcgg cacccaccgg ggcgggatca atatctattc ccccgggctg gagaagttca
     5521 acctgtatcg ccaggaggct tcgcccaata gtctttcgta taatgatgtg aagaccttct
     5581 gcgaggaccg gggcgggaat atctgggtcg gtaccgatgg cggcggcctc gatctgttcc
     5641 tccgggacga gcgggtcttc cggcattacc gcaacgatcc tttcaacgac cggagccttg
     5701 gttcgaatgc ggtgctcgat gtcttccagg accgggaggg tgagttatgg gtttccacct
     5761 ggggtggagg gcttaaccac ttcgaccggc agcggggcga ttttacccgt tatcagaaca
     5821 acccggcaga ccggtactcg atcaccagca atttcgtgca gaagaccttc gaggattttt
     5881 ccgggaatct ctgggtggct acttattatg gcgggctcaa catcttcgac cggaagaaga
     5941 agcaattcac caggctgata gatgatccca cgggcaagac gagcctgacg gggaagaata
     6001 tcgtttcgtt gctggaggac agggagggca ggatctggat cggcaccgat gacggagggc
     6061 tgaactgctg gaacgagaac acacggcatt ttacgcatta tttcgaccag gaggagaaga
     6121 tgcccgatct gcgggtattg ttctgcgata gccatggccg gctttgggtg ggtcagaaag
     6181 ggttgtacct gttcgatgcg gcgcgggaca gtttccggct ttatgcgaac cggtcggggc
     6241 tgtcgaatga atttatcaag gggataacgg aagacgggca gggcaacctc tggatctcca
     6301 cctccaacgg gcttacccag ctgaacgcgg atgggtatgg ttttaagaaa tacaatacgg
     6361 gtgacggatt gcaggacctg gaattcgagg ccaatgcttt tttgaagacc agggatggcg
     6421 agatgtattt cggagggatc aatggcttta acagtttcta tcccggggct attatggcca
     6481 atgcttttgt cccgccggta tatatcaccg ggttccagct gtccaaccgg aagatcggtc
     6541 cgggcaatgg gtcgccgctg gacagggaca tcagtatgat gcgggagatc cggctgtcct
     6601 accgccagtc caccttttcc tttacctatt cggcattgaa ctataccacg ccggagaaca
     6661 accaatatgc gtataaattg gaggggctgg atacggcatg gaactatgtc ggcaaggaga
     6721 ccaaggcggt gtatacgaat cttatgcccg gggattatgt cttccgggtg aaggcgtcca
     6781 ataacgatgg ggtctggaat gaggatggag cgacggtgaa ggtgatcatc accccgccct
     6841 tctggcggac aggatggttt atctcgctgc tggtagcgct tgggatcgcg gggctgtatg
     6901 tgtggtacaa attccggacc cggctgaaga tgcgggagct ggaagaaaag aagcgggagg
     6961 agatgcggca ggtgcagctc cagttcttta ccaatgtctc gcatgagttc cgcacgccgc
     7021 tgtcgctgat cctggggcca ctggagaaga tgatgaagga gtataatacg ccggtgctga
     7081 accggtattt ccaggccatg caccggaatg cgcagcggat gcttagtctt atccatgagc
     7141 tgatggattt tgggaagctg gagagcggat cgttgcggct ttgtgtgcag gcgggcagtc
     7201 tgaatacctt tatggaggaa ctggcggagg agttcaggga ctgggcggta cagaaggaga
     7261 tcgatttttg cctggaaggt ggattgacgg gagaggcctg gtttgaccgg caggtgctgg
     7321 agaagatagt gctcaacctc ttgcagaatt ccttcaagta taccaaggcc gggggtcgga
     7381 tcacgttgca gttgttgggt tcgatggagg ggtttacgcc ctcgtatgcg ggtgagctgg
     7441 ttttgaagaa cccgaaccgc gggagccggt atgcctatat ccgggtcgca gataccggca
     7501 tcggcatctc ggccggcagt atccatcatt tgttcgagcg ctattaccgg atcaccgaat
     7561 cgcatctggg atcgggtgtc gggcttgcct ttgtaaaaag ccttgcgctt ctgcataagg
     7621 gggatattta tgtgtacagc gaacggctga aggggacgga gatcattatc ggtattccta
     7681 taagcgtgga tgattatggg gtcgaagaaa aacgggccat ccatgtggaa ggcggggtgc
     7741 ggctggaagg tttcccggtc acccgcggcg aggcgccggc tgtcaacctg ccgcctgtgg
     7801 aaacgatatt actggtggag gataacgaag agctgcggca tttcctgaag gatagtctta
     7861 gtccttacta ccaggtgatg gaggccgccg atgggcggga ggggctggac aaggcgaggg
     7921 agatcgctcc ggggatgatc atcagcgatg tgatcatgcc ggggatgaat ggcttcgatc
     7981 tttgccgggc agtgaaacag gatatcgata tctgtcatat cccgttcctg atgctgacgg
     8041 cacggtcggc cgcggcggcg cagctggaag ggttggatgc cggggcggac tattattttg
     8101 ccaagccgat gaatatggag ttgttgctgc tgacgatccg caaccggttc gaccaggaca
     8161 ggaaactgaa ggagcggtat tcgcgggagt cgcaggtaga ggcgatggag ctggtgcatt
     8221 cggagaagga ccgggaattc atgcggcggt tgctgaatac catcgattcg cagctgtcga
     8281 acccggattt tgatatcgac tggctttgcc aggagatggg gatgagccgg acgaagctgt
     8341 accagaagat aaaggggatc agtcagcagt ccatcgggga tttcatccgg acgatccggc
     8401 tgaagaaggc ggtgcagatc atgacgcacg aggatgtcac gctgacggag gtgatgatgc
     8461 gcatcggcat ccagacacag tcctatttta cgaaggcctt caagaaggag tttgggaaga
     8521 cgccgacgca gtttatgcag gagatccgga agtaggggcg gcagagcgga caacgggcgt
     8581 atcgaaagcg gacaggtgca tggtgcgacg caggtgtaga aagctgataa ttaccgggat
     8641 ataaaaacgg cattcttttt aggcggatag ggtactatgc ttaaaaacta cttcaagacc
     8701 gccctgcgga acctttggaa gcaaaagggt tttaccgcca tcaatcttac cgggcttgca
     8761 ctgggcatgg cctgtagtct gctgatcctt ctttgggtgc aggatgaacg aagcgtggat
     8821 gggtttcatg taaaggggga ccggcttttt tatgtctatg agcggaacca tatgggagcc
     8881 aggctgcaga gctggtactg gacgcagggg ccactggcgg aggaactgaa gaaagatatc
     8941 ccggaggtgg aggcgtcgac ggtcatcgcc tcgaagtatc cggcggtgtt tggcgtgggt
     9001 gataagaagc tcaaggaaac aggatttgcg gcgcagccgg atttcttttc catgttcagc
     9061 tatccgcttt tgcaggggaa ggcggccgat gcgcttaatt ctcccgatgg gatagctatt
     9121 tcgcagcgga tggcgataga attctttggc agtgcggagg cggccatcgg caaggcaatg
     9181 cgctataacg ataagaaggt gttgatggtg aaggcggtgt atgcggactt ttcgccgcgg
     9241 gtggattatc gcggggattt tatcctgtcg tggagcgggt atatcgaaga cgggaacgat
     9301 tgggcaaaaa actggccttc cgtggaccca tccacctgtg tcctcttgcg gacgggagca
     9361 agtcccgcgg cggtggaggc gaagatgcgg catctgctgg ataaattccc gacggaacag
     9421 aaggatatga agatcgatct gggtttgcag cggtttggcg accgctatct ccattccgat
     9481 ttcaacgccg ccggggtgcc gacgggcggg cggatcgagt atgtgcggct tttcggcatc
     9541 atcgcgttgt ttatccttgt cattgcctgt atcaatttta tgaacctgac cacggcgcgg
     9601 tcggtaaaac gggcgaagga gatcggtgtc cggaaggtga tgggagccgt gcgggggctg
     9661 ctgatccggc agtttatcgg cgaggccgtg ctcatggcct ttttgtccct ccttcttgcg
     9721 ctgatgatcg tgtcgcttgt cctgccggcc ttcaaccagc tgacgggaaa atccatcgcc
     9781 ttgccgatgg gtgatccggg cttttgggct tcacttgggg ggatcaccct gctgacgggt
     9841 ttgttatcgg gcagttaccc ggccttttac ctgtcaggct ttaacccgat acgggtcctc
     9901 aaggccgcgc tgccttcggg gacacggggg gatgccctgt tccggcaggg gctcgtggtc
     9961 tttcaatttg cgttgtccat cgtgctgatc ctggctacca tcctgatcac gcagcagatc
    10021 cattatatgc agaatgcccg gcttggttat gaccgggaga atctcgtcta tctgcccatc
    10081 gagggagaat tggggaagaa gctggaggtg ttgcaggcgg aggcgaaaca gcttccgggt
    10141 attgccaacc tgaccctata ttcgggagat aatccaacgg tgatgggtac tggcacgctt
    10201 ggcatgcgct ggaccggaaa agatccgaat gagcatgatc gctggatcca tgaggccatc
    10261 ggccgggatt ttctttcatc gatgaagatc gagctcgtcg cgggccgtga tttctcaccc
    10321 gactatccga cggacagcaa tggggtcatc gtcaacgaga cggcagtgag actgatgggc
    10381 tataaggacc cgattggcaa a
//
LOCUS       DATGFG010000773         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1293728, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000773 DATGFG010000000
VERSION     DATGFG010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293728"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2217)
                     /locus_tag="VKQ52_14475"
     CDS             complement(<1..>2217)
                     /locus_tag="VKQ52_14475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLZ88457.1"
                     /translation="AVEHMLVLNLHHIAADGRSCDILMQELTFLYSAAASGTTAILPP
                     LPIQYADYAIWQRRWLTSELLAGKLGYWRNQLAGVGPLELPVDHPRPAVQSSRGASLY
                     LPIDDALQKAMQDLSRCSGATLFMTLLAAFKVLLFRYGGQEDICVGTPVTGRTQQETE
                     GLIGFFINTLPLRSNLGGNPSFSELLAAVKNTALAAYDHQEVPFEKMVEEALTERDLA
                     RSPLFQVMFMLQHTLQAPSLRLGDTVLTYEPAGSGQVKFDLTVTITEEEQGLGFHIAY
                     CRDLYEEDTIRRMAVHYVELLRSIAEAPDRRIGELGLLTAAEKQQLTEKYTSAGAGYP
                     DDKTIVDLFASQAARTPEAVALVFEQETVTYRQLDERSNRLAHYLRRLGVKEETPVAI
                     CLEKAPEMIVGILGILKAAGFYVPIDPQYPVHRIGYILGDTAAPVVLTSRGSHAVLPL
                     NYTGQIVRLDEDGPQITAGEDTPPPTLLRPGHLAYVIYTSGSTGQPKGVMIEHRNVIR
                     LFITETPLYDFNERDVWTLFHSFAFDFSVWEIFGALLFGGRLVIVPKAVARDVPAFAR
                     LLTTQGVTVLNQTPSAFYVLQDYLPLMENTLSIRLVIFGGEALDPQKVAPWKRRYPAS
                     RLVNMYGITETTVHVTSLPIGDRHLERSSSVIGTAIPTTTVYILDSRQQLSPMGIAGE
                     IYVGGAGLARGYLNQPALTAEKFIADPFSGKPGARLYKSGDLGRWLPDGTIEYLGRI"
BASE COUNT          450 a          629 c          687 g          451 t
ORIGIN      
        1 gatccggcca aggtattcga tagtgccgtc tggcagccac cggcccagat ctcccgactt
       61 gtagaggcgg gcgccgggtt tcccgctaaa tggatcggcg atgaacttct ctgccgtcag
      121 ggcaggctgg ttgagatagc cccgggccag tcccgctccg ccgacataga tctcgccggc
      181 aattcccatc ggtgatagtt gctgccggct gtccaggata tagaccgtag tggtgggaat
      241 ggctgtacca atgacactgc tgctgcgctc cagatgcctg tccccgatcg gcagagaggt
      301 gacatgaacg gtggtctccg taatgccata catattgacc agcctgcttg cgggataccg
      361 ccgtttccat ggagctacct tctgcggatc gagtgcttct ccaccaaaga tcaccaggcg
      421 gatggagagg gtattttcca tcaggggcag atagtcctgc aagacataga aggcggaggg
      481 agtttgattg agtacggtca cgccctgggt ggtcagtagc ctggcaaagg ccggaacgtc
      541 ccgggccacc gccttgggaa cgatgaccag tcgcccgcca aagagcaatg ccccgaatat
      601 ctcccagacg gaaaaatcga aggcgaaaga atggaatagg gtccatacat cccgctcgtt
      661 gaaatcatac aagggcgttt ccgttatgaa gagcctgatg acattgcggt gttcgatcat
      721 cacccctttg ggttgcccgg tggagccgga ggtatagatg acataggcga gatggccggg
      781 ccgaagcagg gtagggggcg gtgtgtcctc gccggcggtg atctgcggcc cgtcttcatc
      841 cagccggacg atctgtcccg tatagttgag cggcaacacg gcatggctgc cgcggctggt
      901 aagcaccacc ggggcggctg tatccccaag gatataaccg atgcgatgta cagggtattg
      961 cggatcgatg ggaacataga acccggcagc cttgaggatg ccgaggatac cgacgatcat
     1021 ctccggtgct ttttcgaggc agatagccac cggcgtttct tcctttaccc cgagtcgccg
     1081 caggtaatgg gcaagccggt tggagcgctc atccaactgg cggtaagtga ccgtctcctg
     1141 ttcgaacaca agggccaccg cctccggtgt ccgggcagcc tggctggcaa atagatccac
     1201 tattgtcttg tcgtcggggt aaccggcacc ggcgctggtg tacttctccg tcaattgctg
     1261 cttctccgcg gccgtcagta atcccagttc cccgattcgc cggtcgggcg cctcggctat
     1321 cgatcggagc agctccacat aatgaacagc catccgccgg atggtgtctt cctcgtaaag
     1381 gtcccggcag taggctatat ggaatccgag gccctgctcc tcttcagtga tagttacggt
     1441 cagatcgaac ttcacctgtc cgcttccggc gggttcgtag gtaaggaccg tatcgcccag
     1501 gcgcagggac ggtgcctgga gggtgtgctg cagcatgaac atcacctgga ataaaggact
     1561 tcttgccaga tcgcgctcgg tcagggcctc ctccaccatt ttctcgaagg gcacctcctg
     1621 gtggtcatag gctgccaggg ccgtattttt tacggccgcc agcaattcgc tgaaggaagg
     1681 attgccgcca aggttgctgc gcaggggaag ggtattgata aagaagccga tcagcccttc
     1741 ggtctcctgc tgcgtcctgc cggtaacggg cgtgcctaca cagatgtctt cctgcccgcc
     1801 gtaacggaac agcaggacct tgaaggccgc cagcagggtc atgaacaagg tggcgccgga
     1861 acaacgcgag aggtcctgca tcgccttttg aagggcgtcg tcgatcggca ggtacagact
     1921 ggcaccgcgg ctgctctgta ctgccggccg gggatgatcg acgggcagct ccagtgggcc
     1981 cacaccggcc agctggttgc gccagtaccc gagtttgccg gcaaggagct cactggtcag
     2041 ccatcggcgc tgccatatcg catagtcggc gtattggatc ggtaagggag gtaaaatggc
     2101 ggtagtgccc gaggcagcgg ccgaatataa aaaggttagc tcctgcataa ggatatcaca
     2161 ggatcggcca tcggcggcga tatggtgcag gttcagcacc agcatatgtt ccaccgc
//
LOCUS       DATGFG010000774         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_87829, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000774 DATGFG010000000
VERSION     DATGFG010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87829"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VKQ52_14480"
     CDS             complement(<1..1229)
                     /locus_tag="VKQ52_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLZ88458.1"
                     /translation="MTSFDTSHVKNIVLLGHAGSGKTTLSECMLYEAGLTTRRGTVEE
                     KNTVSDFYELEQQRGNSIFSKLLYTEWRGYKINILDTPGYDDFAGDVLSALRVADTGI
                     LLLNANFGVEVGADLIWEYTERLRTPMLLAVNKLDQDKADFDNTVAEAREHFGGNIVV
                     VQYPLNQGLGFNAIIDVLRMTQYRFPPEGGRPEKLPIPEEERERAEGLHKELIEAIAG
                     TDETLMEKYFDKGDLEEDEMRVGLKSAMIRHELFPLFCISAKKNMGSGRIMGFIDAVC
                     PGPAEMPAQRTLSGSALPCNPDGPPCLFVYKTLSEAHIGEMSFFKVYSGTIRPGMELI
                     NEQTGATEKINQLFVVEGGKRIPVNELSAGDIGATIKLKHTHVNNTLHGWGENFELEP
                     IEFPSPLMSIAIESAQKG"
     gene            complement(1332..2552)
                     /locus_tag="VKQ52_14485"
     CDS             complement(1332..2552)
                     /locus_tag="VKQ52_14485"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ88459.1"
                     /translation="MKKNILYIFPLVYMLPLLFCVLSLPAQIAVKGEKVYTLAGAVIT
                     NGVVLIKNGKIEKVGADLKIPANYTVYQARVVTPGLVDPRSEVGMSGPLNIPIDQDQL
                     EKSSPIQPDLRAIDAYNPDDTLVGYLRVNGVTTIHTGHGVGALVSGQTMVAKTKPGLI
                     DDVTLVPTEMLAMSLGSSVQENFTSPGTKSKEIAMLRTELLKAQGYEKKMESKDSSKR
                     PSRDLSQEMLVRLLHGEIKALISANTSAEILSAIRLAREFRLKLVIEGGAEAYRVIDK
                     IKASGAEVIVHPTMARPYGEMANMTMENAAILFRAGIPVSIESGYEGYVPKTRVILFE
                     AAVAAANELPYEEALKAITLNPARLLGLEKRIGSIEKGKDGDIVLYDGDPFEYTTHVC
                     KVIIDGKLVADNCP"
     gene            complement(2584..>2810)
                     /locus_tag="VKQ52_14490"
     CDS             complement(2584..>2810)
                     /locus_tag="VKQ52_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88460.1"
                     /translation="GSLEKGKDADFVILSGDPFSVYTHVEQTWVEGLKRFDRSVPADK
                     AFAVGGYKIYTPESNEEDEDNHNEWSDEKQ"
BASE COUNT          584 a          868 c          695 g          663 t
ORIGIN      
        1 cccttctggg cggattctat cgcaatgctc ataagcggtg aagggaattc aatcggctcg
       61 agctcgaagt tctcccccca gccatgaagc gtattgttga catgcgtatg cttcagcttg
      121 atggtggcgc cgatatcgcc cgccgatagc tcattgacgg gtatgcgctt gccaccctcc
      181 accacaaaaa gttgattgat cttctccgtc gccccggtct gctcattgat cagttccatc
      241 cccggcctga tcgtacccga atacacttta aagaaagaca tttctccgat atgcgcctcc
      301 gaaagcgtct tgtagacaaa caggcagggc ggaccatccg ggttacaggg aagcgcagat
      361 ccggagaggg ttcgctgcgc cggcatctca gccggaccgg gacagacagc atcgataaaa
      421 cccatgatcc gcccgctccc catattcttt ttagccgaaa tacagaaaag agggaacagc
      481 tcatgccgga tcatggcgct tttaagcccc acccgcattt catcctcctc cagatcgccc
      541 ttgtcaaagt atttctccat cagggtctca tcggttcccg cgatggcttc gatcagctct
      601 ttgtgaagcc cttccgcccg ctcccgctcc tcctccggaa taggtagctt ttccggtcgc
      661 ccgccttccg gtgggaatcg atactgcgtc atccgcagga catcgatgat ggcgttaaaa
      721 cccagcccct ggttcagcgg gtattgcacg acgacgatat tgccgccaaa atgctccctg
      781 gcctcggcca ccgtgttgtc gaagtccgcc ttgtcctggt ctaatttgtt gacggccagc
      841 agcatgggtg tacgcaggcg ctcggtatac tcccagatga gatcggcgcc aacctccact
      901 ccgaaattgg cgttcagcaa caggatacca gtatccgcca ctcgcaatgc cgacagcaca
      961 tcgcccgcga aatcatcata ccccggggtg tccaggatat tgatcttgta gccgcgccat
     1021 tccgtgtaca ggagcttgga gaaaatggaa tttccccgct gctgttccaa ttcgtaaaag
     1081 tcgctgacgg tgttcttctc ttctacagtg ccgcgtcgcg tagtgagtcc tgcctcataa
     1141 agcatgcatt ctgacagcgt ggtcttccca gagccggcat gaccgagcaa gactatattc
     1201 ttgacgtgag aggtgtcaaa actagtcatg gcaatcgtat ttcctattaa gttacggaaa
     1261 acgatccttc cgtcaaaatg cgcgtcttac ggtccgccgc atcccgcctt acttcgagcc
     1321 cccgaccccc ctcagggaca attgtccgcc acaagcttcc cgtcgatgat aaccttgcat
     1381 acatgggtgg tatactcgaa cggatcgccg tcatataaca cgatatcccc atccttccct
     1441 ttctcgatac tcccgatccg cttctccagt cccagcaacc gcgcaggatt cagcgttatc
     1501 gccttcagag cctcctcata gggcagctcg ttggccgccg caacggccgc ctcgaacagg
     1561 atcacccgcg tcttcgggac atacccttca taaccgctct cgatcgatac agggatcccc
     1621 gcccggaaca ggatcgccgc attttccatc gtcatattgg ccatctcgcc atacggccgg
     1681 gccatggtgg gatgcacgat gacctctgct cccgaggcct ttatcttatc gatcacccgg
     1741 taggcttccg cacccccttc gatgaccagt ttcaacctga actccctggc aagccggatg
     1801 gcggaaagga tctctgccga cgtattggcg gagataagcg ccttgatctc gccatgcagg
     1861 agcctgacca gcatttcctg gctcagatca cgcgacggtc gcttcgaact gtccttggac
     1921 tccattttct tttcataacc ctgtgccttc aacagctcgg tcctcagcat ggcgatctcc
     1981 ttggacttgg tgcccggcga agtaaagttc tcctgtacac tgctgccgag ggacatagcc
     2041 agcatctcgg taggcacaag ggtgacatca tcgatcagcc ccggctttgt ctttgccacc
     2101 atcgtctgac cgctgacaag cgccccgacg ccatgcccgg tatggatggt ggtcactcca
     2161 ttgacacgca gataaccgac aagggtatca tccgggttat aggcatctat cgcgcgcaga
     2221 tcgggctgta tcgggctgga tttctccagc tggtcctggt cgatagggat gttcaacgga
     2281 ccggacatgc ctacttcgct acgggggtcc accagcccgg gcgtaacgac cctggcctgg
     2341 taaacagtgt agttggcagg gatcttcaga tcggcgccta ctttttcgat cttgccgttc
     2401 tttatcaata cgaccccgtt ggtgatcacc gccccggcca gggtatagac cttctcgcct
     2461 ttaacggcga tctgcgcggg aagggaaagc acgcaaaaga ggagcggaag catataaacg
     2521 agcggaaata tatagaggat gttctttttc atgattcgtc ttttgataga tgtggtagat
     2581 ggcttattgt ttttcatcgc tccattcgtt gtggttgtcc tcgtcctcct cattgctctc
     2641 gggggtatag atcttatatc cgcccacggc aaacgccttg tcagccggca ccgatcggtc
     2701 gaagcgcttg agcccctcca cccaggtctg ctcgacatgc gtatacacgc tgaacggatc
     2761 gcccgacagg atcacgaaat ccgcatcctt acccttttcc agcgagccga
//
LOCUS       DATGFG010000775         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1661764, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000775 DATGFG010000000
VERSION     DATGFG010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661764"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VKQ52_14495"
     CDS             <1..1378
                     /locus_tag="VKQ52_14495"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HLZ88461.1"
                     /translation="HTLASVTSISGTGLHTGIKVDMTLKPATPGFGFQFQRIDLPGRP
                     VIKADCDLVTDTSRGTTLEDNGAKVSTVEHILAALVGLGVDNCLIEINGPEVPIIDGS
                     SQPFVEIIEEAGLTEQEATKVWYSIDSNISHYDEKKRVEMVVMPAMEYKITTLIDFNS
                     PVLGTQHASIKSMRDFKSEISPCRTFCFLHELEMLLEHNLIKGGDINNAIVVVDKPVT
                     QEEMGRLAKAFRRDKIEIKTEGYLNNLELRFPNEPARHKLLDIIGDLALIGYPIKGQI
                     IANRPGHSTNVEFARKIKQYIKKNKHLKNTPVYDANQAPVFTTTQIEKTLPHRFPFLL
                     VDKIIELTDRHIVGIKNVTFNEPFFVGHFPGNPVMPGVLQIEALAQTGGILCINLMPE
                     GQYDTYFIKIDNCKFKHKVVPGDTMLLKMELLNPIRRGICEMRGTVYVGNKIATEADL
                     MAQLVKRS"
     gene            1515..2321
                     /gene="lpxA"
                     /locus_tag="VKQ52_14500"
     CDS             1515..2321
                     /gene="lpxA"
                     /locus_tag="VKQ52_14500"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HLZ88462.1"
                     /translation="MIHSLTSIHPDARLAPDVRVDPFTMIHKNVEIGAGTWIGSNVTI
                     MEGARIGKNCRIFPGAVVSADPQDLKFDGEETTVQIGDNTTIREFVTIHRGTRDRWTT
                     RVGRNCMILAYSHIAHDCIIGDNCILSNNSQVAGHVTLGDWVILGGMCAIHQFVKVGS
                     HVFLGGGSLVGKDIPPYIKAARMPLSYSGVNSVGLKRRGFTIDKINHILDIYRVIYNK
                     GMNTSQALEFLEEEFPASDERDEIVAFIRESGRGIIKRFSKSSFDEDYAD"
     gene            2302..>2856
                     /locus_tag="VKQ52_14505"
     CDS             2302..>2856
                     /locus_tag="VKQ52_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ88463.1"
                     /translation="MRITLIDAGKRFNRDWIFRHLSYEFSPSHSYAITGPNGSGKSTL
                     LQSIAGAIAISEGAIRYEGSDEIPPELAYRRLSLCTPYLELVEEMTVTEFLQFHGSFK
                     PFLPGITVGGIIQKMGLEAAAHKQMRYYSSGMKQRVRLAQALFSATPVVLLDEPCTNL
                     DVEGIALYRRLIAEYGGGRMLVISS"
BASE COUNT          698 a          807 c          750 g          601 t
ORIGIN      
        1 acacacatta gcttctgtaa caagtatttc cggcacaggc ctgcacactg gcataaaggt
       61 ggatatgacc ctgaaacccg cgaccccggg ttttggcttt cagtttcagc ggatcgacct
      121 acccggccgg cccgtcatca aggcggactg tgacctggtg accgatacct cccggggcac
      181 taccctggaa gacaatggcg cgaaggtcag cacggtggag catatcctgg cggccctcgt
      241 cggattgggt gtggacaatt gcctgatcga gatcaacggc cctgaagttc cgatcatcga
      301 cggcagctcg cagccttttg tcgagatcat cgaagaagca ggcctgaccg agcaggaggc
      361 gacaaaggtc tggtacagca tcgactccaa catctcccat tatgacgaaa agaaaagggt
      421 ggaaatggta gtcatgcccg ccatggaata taagatcacc acccttatcg acttcaacag
      481 cccggtcctc ggcacccagc acgccagcat caagtccatg cgtgatttca agagcgagat
      541 atctccctgc cgcaccttct gcttcctgca tgaactggag atgctgctgg aacataacct
      601 tataaaaggc ggtgacatca acaatgcgat cgtggtggtc gataaacccg tcacccagga
      661 ggaaatgggc cgcctggcca aggctttccg gcgcgataag atcgagatca agaccgaagg
      721 ctatctcaac aacctcgaat tgcggtttcc caacgaaccc gcgcggcaca aactgctgga
      781 catcatcggc gacctggccc tgatcggcta tcccataaaa ggccagatca tcgccaaccg
      841 gcccgggcac tccaccaatg tcgagttcgc ccgcaagatc aagcaataca taaagaagaa
      901 caagcatctc aagaataccc cggtgtacga tgccaaccag gcgccagtct ttacgactac
      961 ccagattgaa aagaccttgc cgcaccgctt tcccttcctg ctggtcgaca agatcatcga
     1021 gctgaccgac cggcacattg tcgggataaa gaacgtcact ttcaacgaac cattcttcgt
     1081 cggccacttc cccggcaacc ccgtcatgcc tggtgtgctg cagatcgaag ccctggccca
     1141 gaccggcggc atcctctgta tcaacctgat gcccgaaggc cagtacgata cctactttat
     1201 caagatcgat aactgcaaat tcaaacacaa ggtcgttccg ggggatacca tgctcctgaa
     1261 gatggagctc ctcaacccga tacgccgtgg catctgcgaa atgcgtggaa cggtgtatgt
     1321 tggcaacaaa attgctacag aagcggacct aatggcccaa ttagtaaaac ggagttaatg
     1381 acttgtaaag agtagttaac cggtagtaaa cagaagttta tttcgggtat ttgtgtcaaa
     1441 taagattatt tttgtggcac aaggaagaaa ccataatgat ccaagaaact caaaatcaat
     1501 caattgatcg tcgaatgatc cactctctca cctccattca cccggacgcc cgcctcgcgc
     1561 ccgatgtacg agtggacccc ttcacgatga tccataagaa tgtagaaatt ggagcaggga
     1621 cctggatcgg cagcaatgtg acgattatgg agggtgcgag gatcggtaag aactgcagga
     1681 tcttccctgg ggccgtggta tccgctgatc ctcaagacct taagtttgac ggcgaagaaa
     1741 cgacggtaca aataggcgac aatacgacca tccgggagtt tgtgaccatc caccgcggca
     1801 cccgcgaccg ctggacgacc cgggtgggcc gcaactgtat gatcctcgcc tatagccata
     1861 tcgcgcacga ctgtatcatc ggcgacaact gcattctcag caataactcc caggtggcgg
     1921 ggcatgttac gcttggcgac tgggtcatcc tcggcgggat gtgcgccatc caccagttcg
     1981 taaaagtcgg ttctcatgtc tttctgggtg gcggttccct tgtaggcaag gatatccccc
     2041 cctatatcaa ggcggcccgg atgccgctga gctattccgg cgtcaactcc gttgggctta
     2101 agcgccgggg ttttaccatc gacaagatca accatatcct cgatatctac cgggtgatct
     2161 acaataaggg tatgaatacc agccaggccc tcgaattcct ggaagaggag ttccccgcca
     2221 gcgatgaacg ggacgagatt gtcgccttca tccgggaaag cggacgcggt attattaaac
     2281 gcttcagcaa atcgagcttt gatgaggatt acgctgattg atgccggtaa acggttcaac
     2341 cgcgactgga tcttccgcca tctcagttac gaattttccc cttcccatag ttatgcgatc
     2401 accggcccca acgggtccgg gaagtccacg ttgttacagt ccattgccgg ggccatcgcc
     2461 atcagtgagg gggctatccg gtatgaaggg tcggacgaga tccccccgga gctggcctat
     2521 cgccgtctgt ccctttgcac accctatctg gaactggtcg aagagatgac cgtcaccgaa
     2581 ttcctgcagt tccatggcag tttcaaaccc tttcttccgg ggatcaccgt cgggggcatt
     2641 attcaaaaga tggggctcga agccgcggcg cataagcaga tgcgttacta ttcttccggg
     2701 atgaaacaac gggtgcggct ggcccaggcc cttttctcgg cgacgccggt ggtgctgctc
     2761 gatgagccct gtaccaacct ggatgtggag ggtatcgcgc tttaccggag gcttatcgcc
     2821 gaatatgggg ggggcaggat gctggtcatc agcagc
//
LOCUS       DATGFG010000776         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1041491, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000776 DATGFG010000000
VERSION     DATGFG010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041491"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..992)
                     /locus_tag="VKQ52_14510"
     CDS             complement(171..992)
                     /locus_tag="VKQ52_14510"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HLZ88464.1"
                     /translation="MALITLTSDIGQKDYLVGAIKGQLIQIDPAFQLMDISHELSPFN
                     YPQAAYVCRNAIRHYPPFTFHIILVNLFEKKPDQLILAFHKEQYLLCADNGLLNMILE
                     GRPEMVIGLPLDKTAVKNTLYCVRVMGQAISQLAKGAGLLSIGIPDIPFTEKNHLRPV
                     FGDNWIEGQIIFIDHFENVIVNITREEFEQQRRGRGFRIVFKRDETIDKISETYADVG
                     EGEKLALFNSAGYLEIAIQKGNAAGLFGLQDFTEQSQNQYLQSRLFYQTVKVFFE"
     gene            complement(998..1807)
                     /locus_tag="VKQ52_14515"
     CDS             complement(998..1807)
                     /locus_tag="VKQ52_14515"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ88465.1"
                     /translation="MSFFQDKVVVITGGSDGIGKALIEALIPLGAKVATCGRSHDKIY
                     KLQMEYARVMLHAVACDVSNEEECCRFIESTIETFGHIDILINNAGISMRALFTECDT
                     KVTRKVMEINFLGAVYCTKYALPSILENKGTIVGVSSTAGYRGLPGRSAYSASKFALQ
                     GWLESLRTELLHSGVHVMWVCPGFTASNIRLAALDPHGDARGETVLNEASLMSAEKCA
                     RHVLRAIQRRKRTLVLTFLGKVTVFINKMAPSLADRLTYNHYYNKKGEFVK"
     gene            complement(1903..2397)
                     /locus_tag="VKQ52_14520"
     CDS             complement(1903..2397)
                     /locus_tag="VKQ52_14520"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ88466.1"
                     /translation="MTTTTTQQDLLVSTGLNSWEQAIRRATAFFDGCTDEDLEKEILP
                     GKNRVVYLLGHLVAVHDRMLPLLGLGERLYPHLDALFVTNPDNPAASLPPAADLRSQW
                     TTINHRLTAAFSHWTPEEWLQKHTAVSEEDFAKEPHRNRFSVVINRAGHVQYHLGQLV
                     WFKK"
     gene            2498..3052
                     /locus_tag="VKQ52_14525"
     CDS             2498..3052
                     /locus_tag="VKQ52_14525"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HLZ88467.1"
                     /translation="MKKNLLFCLAGLLLLSACNLGNKKPTNSEIIKKAAGMPGVNAGS
                     GNFDIDAPSGWTRIDTSISGLKATLLMSSDASKGFRSNVNVITQAMQGMSDDSYFDAN
                     VVGLRSNLAQFQTIDKGQKQVNGMNARWIHYSGLNNGMMLEQMLYIIPSGGVAYLITC
                     TSLQGQMAKDQSNFDVAVNSFKVH"
     gene            complement(3055..3876)
                     /locus_tag="VKQ52_14530"
     CDS             complement(3055..3876)
                     /locus_tag="VKQ52_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ88468.1"
                     /translation="MKKAIHLLAAIATISLLVASCVPTRRWKTSEAALASARADSARL
                     AQQVAEHEATIGQLKQQIGDLNKKIDDLQNQSGLTQSQLNQSKEQIAEQRRRLAQLQA
                     LMDQQKRAIQDIRKKMTDALVGFNSKDLTVSIKNGKVYVSLSENLLFPSGSAVVNPKG
                     KEALGKLAQVLNTNPDITVDIEGHTDTVPIHGRYQDNWALSVARSTAIVRILTTDYMV
                     DPIRVIASGHSSYDPVQPNSTPEGRALNRRTEIILSPKLDELYKLLESGPDESAP"
BASE COUNT          784 a          867 c         1304 g         1002 t
ORIGIN      
        1 tgtcgcgaag aggcctacta tccatccaat gatgttgttg actttggtga agcgcatggt
       61 tgggcttttt ctacgttttg ggcacgaatt aaggccaaat agggcaaatt tccgcgggaa
      121 tgggggtaaa ttttagtaaa ggtgctgtta atcaggtgtt ttgggcgcga ttattcgaag
      181 aagactttta cggtttggta gaacaggcgg ctttggaggt attgattctg ggattgttcg
      241 gtgaaatcct gcaagccgaa gaggccggcg gcgttgcctt tctggatggc gatctcgagg
      301 tagccggcgg agttgaagag cgccagtttc tcgccttcgc caacgtccgc ataggtttcg
      361 cttattttat cgatggtctc gtcgcgtttg aagacgatgc ggaagccgcg gccccggcgt
      421 tgctgttcga attcttcgcg ggtgatgttg acgatgacgt tctcgaagtg atcgatgaag
      481 atgatctggc cttcgatcca gttgtccccg aatacggggc gcaggtggtt cttttcggta
      541 aaggggatgt cggggatgcc gatactgagg aggccggcgc cttttgccaa ttgggagatg
      601 gcctggccca tgacccggac gcagtagagg gtgttcttga cggctgtttt gtcgaggggc
      661 aggccgatga ccatttcggg gcggccttcg aggatcatgt tgagcaggcc gttatcggcg
      721 cagagcaggt attgttcttt atggaaggcc aggatgagtt ggtcgggctt tttttcgaaa
      781 aggttgacca ggatgatatg aaaggtaaag ggtgggtaat ggcggatggc attacggcag
      841 acataggccg cctggggata gttgaagggt gacagctcat gggagatgtc catcagttgg
      901 aaggcggggt ctatttgtat gagttggcct tttatagccc cgacgagata atctttctga
      961 ccgatatctg atgtaagggt gattaaggcc atgccggtta tttaacgaac tcccctttct
     1021 tattgtaata atgattatag gtgagcctgt cagccaggga gggcgccatt ttgttgatga
     1081 acacggtgac ctttccgagg aaggtgagta cgagggttcg tttgcggcgt tggatggccc
     1141 gcaggacgtg gcgggcgcat ttttcggcgc tcatcaggct ggcttcgttg agtaccgttt
     1201 cgccgcgggc gtcgccgtgt gggtcgaggg cggccaggcg tatattggag gccgtgaaac
     1261 ccgggcagac ccacatcacg tggacgccgc tgtgcagcag ttcggtgcgg agggattcca
     1321 gccagccctg cagggcgaat ttcgatgccg agtatgcgct tcgtccgggg aggccgcgat
     1381 agcctgccgt ggaagagacg ccgacgatgg tgcctttgtt ctccaggatg gaaggcagtg
     1441 cgtatttggt gcagtatacg gcgccgagga aatttatctc catgaccttc cgggtcactt
     1501 tcgtatcgca ttccgtgaag agggcacgca tggagatacc ggcgttgttg atcaggatgt
     1561 cgatatggcc gaaggtttcg atggtcgatt cgatgaagcg gcagcattct tcttcgttgc
     1621 tgacgtcgca ggccacggcg tggagcataa cccgcgcgta ttccatctgg agcttataga
     1681 tcttatcatg gctacggcca caggtagcca ctttggcgcc gaggggaata agggcttcta
     1741 tcagcgcctt accgatgccg tcgctgccac ctgtgatcac gactacttta tcctggaaga
     1801 aagacatgga tggagtttgg ttattggggc aaaagtaaga tacaaaaacc taaaaaccat
     1861 gtcgtcgtgg gcagaagggc agcgacggga tgaagaaagg aactattttt tgaaccagac
     1921 gagctggccg agatggtatt ggacgtgtcc tgcccggttg ataacgacgc tgaaccgatt
     1981 gcggtggggc tcttttgcga agtcttcttc ggagacggcg gtgtgttttt ggagccattc
     2041 ttccggtgtc cagtggctga aggcggcggt gagccggtgg ttgatggtgg tccactggct
     2101 gcggaggtcg gcggcggggg gcaggcttgc cgcggggtta tccgggttgg ttacgaaaag
     2161 ggcgtccaga tgggggtaga gccgttcgcc caggccgagg aggggaagca tacggtcgtg
     2221 gacggctacc aggtggccca aaaggtagac gacccggttt ttgcccggga ggatctcttt
     2281 ttcgaggtct tcatcggtgc agccgtcgaa aaaggcggtg gctcttctta tcgcctgttc
     2341 ccaggagttg aggccggtgg agacgagcag gtcctgctga gtggtggtgg ttgtcatgtt
     2401 attttattaa gatagtcata ctaacaaggt gatcccggat ttgtttatac ggggcaaggg
     2461 cttatcttag tatatctttc catcaatacc tcctaatatg aaaaagaacc tgctcttttg
     2521 tcttgccggc ctccttttgt tgtcggcctg taatctgggc aataagaagc caaccaatag
     2581 tgagatcata aaaaaggccg ccgggatgcc cggtgtcaat gccggttcgg gtaatttcga
     2641 tatcgatgct ccctcgggct ggacgcggat cgatacgagc atttccggtc tgaaggccac
     2701 gctgctgatg tcttcggatg ccagcaaggg attccggtcg aatgtcaatg tgatcaccca
     2761 ggcgatgcag gggatgtcag acgacagtta tttcgacgcc aatgtcgttg ggctgcgcag
     2821 taacctggcg cagtttcaga ccatcgacaa gggacagaag caggtgaacg ggatgaatgc
     2881 ccgttggatc cattattccg ggctgaacaa tgggatgatg ctggagcaga tgctgtatat
     2941 cattcccagt ggcggagtgg cctatctgat cacctgtact tcgttgcagg ggcagatggc
     3001 gaaggatcag tcgaatttcg atgtggcggt gaattctttt aaagtacatt aaaattaagg
     3061 ggccgattcg tcggggcccg attcgaggag tttatacagt tcgtcgagtt ttggcgagag
     3121 gatgatctcg gtgcggcggt tgagggcgcg gccttcgggt gtgctgttcg gctgtacggg
     3181 atcataggag ctgtggccgg aggcgatcac ccggatgggg tcaaccatgt aatcggtggt
     3241 gaggatgcgg acgatggccg tggagcgggc cacgctgagg gcccagttat cctggtaacg
     3301 accgtggatg gggacggtgt cggtgtggcc ttcgatgtct acggtgatat ccgggttggt
     3361 gttcagcacc tgggcaagct taccgagggc ttccttccct ttggggttga cgacggcgct
     3421 gccggagggg aagagcaggt tttccgacag gctcacgtag accttgccgt tcttgatgga
     3481 tacggtgagg tctttggagt tgaagccgac gagggcgtcg gtcattttct tccggatgtc
     3541 ctgaatggcg cgtttttgct ggtccatcag cgcctgcaac tgggctagcc gccgccgttg
     3601 ttcggcgatc tgttccttac tttggttgag ttggctctgg gtgaggccgg actggttctg
     3661 gagatcgtct attttcttat tcaaatcccc aatctgttgt tttaattgcc caatggtcgc
     3721 ttcgtgttcg gccacctgct gtgccagccg ggcactgtcg gcgcgggcgg aggcgagcgc
     3781 ggcctcggag gtcttccatc ggcgggtggg cacgcaactg gctactaata aggagattgt
     3841 ggcgatagca gccaggaggt gaatggcttt tttcatacat gtagattatt gttaaacaat
     3901 gtgtagtacc acagtttctg aaacaatgat caggccattg cggggaaaga ggatcgg
//
LOCUS       DATGFG010000777         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1209540, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000777 DATGFG010000000
VERSION     DATGFG010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209540"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            584..2074
                     /locus_tag="VKQ52_14535"
     CDS             584..2074
                     /locus_tag="VKQ52_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88469.1"
                     /translation="MRKIPAFVGAALLCLTLQATHAQQIDSFSNKVLTFPSRWLGHIQ
                     SRTASLNQQLTSQTEKMLQKMAQREARMEKKLSGVDSLGARQLFAHSQQEYASLLQQM
                     RADTGLHRQRATGQYQPYVDTLQGSLSFLKQNPRLLGGTTNNLNPEIQGQLQGASSQI
                     QSIQARLQVASQAKAFVQQRKTLINQYISQHVNMQSLLNQPYSAMNQEVYYYSQRVQQ
                     YKDMLNSPDKLEKQALARLGTMPAFRNFMKNNSQLSGLFKLPGAGFGGGTAQPLPGLQ
                     THAQVAQQVQGQVGASGPAGLDAFQSKIQSAQTQLNSYKSKISQLGAGSTVADVPDFR
                     PNEQKTKTLWHRLEYGANFQTTHTNYYYPVVTDLGLSLGYRLGHGKIVGIGASYKMGW
                     GSGFNHISITSQGIGLRSFLQMPIKGSFSATGGFEYNYATPFASYQQLKQLSLWTKSG
                     LIGVTKTVSMKSRVFKKTQLQLLWDFLSYQQVPKTQALLFRIGYAF"
     gene            2156..>2414
                     /locus_tag="VKQ52_14540"
     CDS             2156..>2414
                     /locus_tag="VKQ52_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88470.1"
                     /translation="MILASLPFLATGQQQVGSPNTMPDISSPAIVSPNAAALGKYGEL
                     PVSYYTGIPNINIPLYEIKTGSLDLPISLSYHAGGIKVEEIA"
BASE COUNT          579 a          638 c          601 g          596 t
ORIGIN      
        1 agcgaggtgt tgcccgccta tccgtactgg catcagctgc agttcccgat gttgcatacg
       61 gcgccggagt tgtatcggtg agatttgagt gaggatttgg agtattcgga atttgcgaat
      121 tttcgcaaag ggggaaggta ttttcacggt atttttttcg cgctttaact acaaattgtc
      181 ttttgggggt gagcggctca tttcacgttc accctttttt tatgcataac cgacatgggt
      241 ttcagcacgg tcatcagaat aaaccgttgg atcatccacc gccgggtttg ccctcttcgg
      301 gtatccggtt ctccgcacaa atagggcatt aacggatacc cctagaaaaa tataaaaatt
      361 ttttacttac ttcgcatcat acctctccaa cctttccctt tagcatccta cccgctccat
      421 ttaaccttct tatcgaacct caaaccgtct gaattatgac tcctttttgc gcgcgtatgc
      481 gtggaccctc atccgttcct tcgcttttat ctccacccaa tttttattgt aagatccggc
      541 tgcgggattc ccggagctaa gtctattctg taattccggc tatatgcgaa aaattccggc
      601 ttttgtcggg gcggccctgc tatgcctcac cctgcaggct acccatgccc aacagatcga
      661 ttcctttagc aataaggtac tgaccttccc atcccgatgg ttaggccata tccaatcgcg
      721 gacggccagc ctcaaccagc agttgacgag tcaaacggag aaaatgctgc aaaagatggc
      781 gcagcgcgaa gcccggatgg agaagaaatt atcgggtgtc gatagcctgg gcgcccggca
      841 actatttgcg cattcccaac aggaatacgc ctcccttctg caacaaatgc gggcggacac
      901 cggccttcat cgtcagcggg cgaccggcca atatcaaccc tatgtagaca ccctgcaggg
      961 gtctctttcc tttctcaaac agaacccgcg gttattgggg ggaacgacaa acaacctcaa
     1021 cccggagatc cagggtcagt tacagggcgc aagcagccaa attcaatcca tacaggcgag
     1081 gctgcaggta gccagccagg caaaggcctt tgtacagcaa cgaaagacgc tgatcaacca
     1141 atatattagc cagcatgtca atatgcaatc cctgcttaat caaccctatt ccgcgatgaa
     1201 tcaggaggtg tactattact cgcagcgggt gcagcaatac aaggacatgc tgaacagccc
     1261 ggataaactg gaaaagcagg cgctggcgag gcttggtacc atgcctgctt ttcggaattt
     1321 catgaagaac aactcgcagc tgagtggtct tttcaaactg cccggcgctg gttttggtgg
     1381 cggcactgct caaccgcttc ccggattgca aactcacgcc caggtggcgc aacaggtgca
     1441 gggccaggtc ggcgcttcgg gtcctgcggg cctcgacgct tttcaatcca aaatccaatc
     1501 ggcgcagacg cagctcaaca gctataaaag caagatcagc cagcttggtg cgggctccac
     1561 cgtggccgat gtccccgatt tccggccgaa tgagcagaaa acaaagacgc tctggcatcg
     1621 gctcgaatac ggcgccaatt tccagactac ccataccaac tattattacc cagtggtgac
     1681 cgatcttggt ctgtcgctag gctaccgtct tggtcatggc aaaatagttg gcatcggcgc
     1741 cagctacaaa atggggtggg ggagcggctt caatcatatc tcgatcacca gccagggtat
     1801 tggccttcgc tccttccttc aaatgccgat taaaggtagt ttctcggcaa cgggcgggtt
     1861 cgagtacaat tatgccacac cgtttgcctc ctaccagcag ttaaagcagt tgtcactatg
     1921 gacgaagtcg gggctgatcg gcgtgacaaa gacggtgtcg atgaagagcc gggtgttcaa
     1981 gaagacgcag ttgcagctgt tgtgggattt tttgagttat cagcaggtgc cgaagacgca
     2041 ggcgcttttg tttcggatag gctatgcgtt ttgagccctg cttcatctta ttcaactata
     2101 acgacaatta attcatagga agaacatgag agccagtgtt tcaatttgta cgatcttgat
     2161 tcttgcatcg ttgccattcc tggccaccgg acagcagcag gtgggtagtc ctaatacgat
     2221 gccggatatt tccagcccgg ccattgtatc acccaatgca gccgctttgg ggaaatatgg
     2281 ggaattgccg gtaagttatt atacgggtat tcccaatatc aatatccctc tatatgaaat
     2341 aaagaccggc tccctggacc tgccgatcag tctgtcgtat catgccggcg ggatcaaagt
     2401 ggaagagatt gctt
//
LOCUS       DATGFG010000778         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_955381, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000778 DATGFG010000000
VERSION     DATGFG010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955381"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VKQ52_14545"
     CDS             <1..1036
                     /locus_tag="VKQ52_14545"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HLZ88471.1"
                     /translation="DANQAGEDNRNVARMAALLAGLPVTVPGNTVNRLCASGLQAIMD
                     AARAIACGDGDLFIAGGVESMTRAPFVMGKSSSAFSRQSEVFDSTIGWRFVNKRMVEM
                     HHPYSMGETAENVARQWKVPREAQDVFGLASQEKYFAALEAGRWADEIAPVEILGGKL
                     EKIYFSTDEQPRHSSMEKMAALRPAFVKDGSVTAGNSAGINDGAAAMLLAGESAVKRF
                     GLKPRARVVSMAVAGVDPAIMGVGPIPATRKALARAGLQVGDLGLIELNEAFAVQALV
                     CMQELGLDGSKVNVNGGSIAIGHPLGCSGVRISTTLVHEMGRRPEVRYGLATMCVGVG
                     QGAAVVYERV"
     gene            complement(1173..1535)
                     /locus_tag="VKQ52_14550"
     CDS             complement(1173..1535)
                     /locus_tag="VKQ52_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88472.1"
                     /translation="MKILSLEPFVPSGSDFHRSKQFFQELGFHINWEADGYAGLECDG
                     CRFILQHYDKVEFAQNFMLSVKVTDVDEFRKMVLDKQLPEKYSIRIGNIRPQPYGREV
                     NIIDIAGVCWHFVEQIRQ"
     gene            1618..2202
                     /locus_tag="VKQ52_14555"
     CDS             1618..2202
                     /locus_tag="VKQ52_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88473.1"
                     /translation="MEFRTKVVTRRRNKRAYIAYAGLVIAGSSLLLVFIPSMNDYIPY
                     VFGIGIAVVVVGAVIARGDVRNYGLSQDELIVSMTGITVGAIHYPLRMVKNMNFNVEA
                     YNGLYVNDGAMVSGSKSDGMTNDLSFESGGSRVAVGFYLGSREHVQLLGAVFDEFYRA
                     RIPFIERNRGRQTFLFQHLTGVELEEFKRKYGYA"
     gene            complement(2212..2763)
                     /gene="rhuM"
                     /locus_tag="VKQ52_14560"
                     /pseudo
     CDS             complement(2212..2763)
                     /gene="rhuM"
                     /locus_tag="VKQ52_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024706.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(2776..3303)
                     /gene="rhuM"
                     /locus_tag="VKQ52_14565"
     CDS             complement(2776..3303)
                     /gene="rhuM"
                     /locus_tag="VKQ52_14565"
                     /inference="COORDINATES: protein motif:HMM:NF024706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HLZ88474.1"
                     /translation="MQTFEILLYTAPEGQTHIEVFYKEESFWLSQKKMAELFGVDVRT
                     ISEHLQNIFKTKELEETSVIRNFRITASDGKNYQTKFYNLDAIIAVGYRVNSIEATRF
                     RIWATKTLREYIIKGFVLDDERLKQGKNFGKDYFDELLERIRDQRRRRRLGRRPIVRS
                     FAEMRQIKEKRISGK"
     gene            complement(3441..>3625)
                     /locus_tag="VKQ52_14570"
     CDS             complement(3441..>3625)
                     /locus_tag="VKQ52_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLZ88475.1"
                     /translation="ILCYEMTGQPLPPNHGAPLRLIIPVKYGVKNLKRIGKMHFSNTR
                     PRDYWFERGYDYYCGL"
BASE COUNT          686 a          711 c         1303 g          925 t
ORIGIN      
        1 ggatgcgaac caggcggggg aagataaccg gaatgtggcg cggatggcgg ccttgctggc
       61 ggggctgccg gtgacggtgc cgggcaatac ggtgaaccgg ctttgtgcgt cggggttaca
      121 ggcgataatg gatgccgccc gggcgatcgc ctgtggggat ggcgatctgt tcatcgctgg
      181 gggtgtggag agcatgacgc gggcgccgtt tgtgatgggg aagagttcgt cggctttcag
      241 ccgccagtcg gaggtgttcg attcgaccat cgggtggcgg tttgtcaata agcggatggt
      301 ggagatgcac catccttatt ccatgggcga gaccgcggag aatgtggcgc ggcagtggaa
      361 ggtgccgcgg gaggcgcagg atgtttttgg gctggcgtcg caggagaaat attttgccgc
      421 gctggaggcg gggaggtggg ccgatgagat cgcgccggtg gagatcctgg ggggtaagct
      481 ggagaagatc tatttctcga cggatgagca gccgcgtcat agttcgatgg aaaagatggc
      541 ggcgttgcgg cctgcttttg taaaagacgg gtcggtgacg gcggggaact ccgcggggat
      601 caatgacggg gccgcggcga tgttgctggc gggggaatcg gcggtgaagc ggtttggatt
      661 gaagccgcgg gcgcgggtgg tgagtatggc ggtggcgggg gtagacccgg cgatcatggg
      721 ggtggggccg ataccggcga cgcggaaggc gcttgcgcgg gcggggctgc aggttggcga
      781 cctgggactg atcgagttga acgaggcgtt tgcggtgcag gcccttgttt gtatgcagga
      841 gttggggctg gatgggtcga aggtgaatgt gaatggtggg tctattgcga tcgggcatcc
      901 actggggtgc agtggggtgc ggatatcgac gacgctggtg catgagatgg ggcggcggcc
      961 ggaggtgcgg tatgggctgg cgacgatgtg cgtgggggtg ggccaggggg ctgcagtggt
     1021 gtatgagagg gtgtaggggt gtgggagatg ggggcgcccg tgatggggct gggagatggg
     1081 agacgggatc gccgggtgat ggggtccggg ggagatggga gacgggatcg tcgggagatg
     1141 gggtccgtgg atgatggggt cgccgggtga tgctactggc ggatctgttc gacaaagtgc
     1201 cagcaaactc cggctatatc gatgatattg acctcccggc catagggttg cggccggata
     1261 ttccctatcc ggatactgta cttttcgggc aattgcttat ccagtaccat ttttctgaat
     1321 tcgtcgacat cggtgacctt aacgctaagc ataaaatttt gggcgaattc tactttgtcg
     1381 tagtgctgga ggatgaaccg gcaaccgtca cattccagcc cggcatagcc atcggcttcc
     1441 caattaatat gaaagcccag ctcctggaag aactgcttgg agcgatggaa gtcgctgccg
     1501 gaaggaacga acggttcgag agaaaggatc ttcatgatag aggtttatgg gctcgaatgt
     1561 aagcattatg ccggctatct ggtacatgag ggtgggtgaa aatttgtaaa ttcggggatg
     1621 gaatttcgta caaaagttgt tacccgtcgc cggaacaagc gggcatatat agcgtatgcc
     1681 ggattggtca tcgccggctc gtcgctgttg ctggtgttta ttccttcgat gaacgattat
     1741 attccttatg tttttgggat cgggatcgcg gttgtcgtcg tcggggcggt gatcgcgcgg
     1801 ggcgatgtcc ggaattacgg gctttcgcag gatgagctga tcgtgagcat gaccgggatc
     1861 acggtggggg cgattcatta ccccttgcgg atggtgaaga acatgaattt taatgtggag
     1921 gcctataacg gactttatgt gaatgacggg gccatggtgt cggggagcaa gtccgatggg
     1981 atgaccaacg atctgtcgtt cgaatccggg ggcagcaggg ttgccgtagg gttttatctc
     2041 gggagcaggg agcatgtaca gctgcttggc gctgtattcg atgagtttta ccgggcgcgt
     2101 atcccgttca tcgagcggaa ccgcggccgg cagacctttt tgttccagca tttgacgggg
     2161 gtggagttgg aggagtttaa gcggaagtat gggtatgcgt gaaggagggg gttattgggg
     2221 cggtggggta gtcgtgaggt cttttatggc ttcgtcgaag tctgatagat aatgttgatc
     2281 ttgtatgacc cggtattggg cgaattgttc ttcgaccagc tttttggcga tgtcggcgct
     2341 gatgcggccc gcgtccttta gtacggcata ttcgttgaat tgcaggaagg cgtcgaggcg
     2401 ctgggcccag tccttcatgg tcatggccat ttttcgtttg gcctggtttt ccgcataatc
     2461 gagatacatg cttacaatgc gattgagctc atcgagttct ttttcggaga ggtagttttt
     2521 ggctattcct atatcggatt tttgaatttt ccctttaggt gcgtttttcc aggtggtgag
     2581 gcccatattg ggtttgtcgg catgggctct ttcggcaatg atctcggcgg cggtgtggcc
     2641 ggtgatggcc caatggagtt tgttttgtac ggttttatag aaagcatggg tgatctcgga
     2701 ttgggggtcg tagtccatgc tgcactgggc gaagatgtcc gtgatcttct ggtagaatct
     2761 tctttcgctg gccctttatt tgccggaaat gcgcttttct ttaatctggc gcatttcggc
     2821 aaatgatctg acgattggcc ttcggccgag acgcctccgg cgtctttggt cccgaatccg
     2881 ttcgagcagt tcgtcgaaat agtctttgcc gaagtttttc ccttgtttca ggcgttcgtc
     2941 gtcgaggaca aatcctttta tgatgtattc ccggagggtt ttagtggccc agatgcggaa
     3001 acgggtggct tcgatggagt tgacgcggta gccgacggcg atgatggcgt cgaggttgta
     3061 gaatttggtt tggtagtttt taccgtcgga ggcagttatc cggaaattcc ggataactga
     3121 ggtttcttct agttctttgg ttttgaatat gttttgaagg tgttcgctga tagtgcgaac
     3181 gtctacgccg aacaattcag ccattttctt ttgcgaaagc cagaaggatt cctctttgta
     3241 gaagacctcg atatgcgttt gtccctcggg cgccgtgtac aacaagatct cgaatgtttg
     3301 cataagggga gctttgcggc aaaaaatgat gccggaggat aaatttagaa aaagggcgga
     3361 tgcaaaaaaa ataaaatgag ggttggcagg ggcgtattgc agggtgtatt agaaatgaaa
     3421 ggggtagcgg gtgagcggag ttagaggccg cagtaatagt cgtagccgcg ttcgaaccag
     3481 tagtcgcggg ggcgggtgtt gctgaagtgc attttgccga tgcgtttgag gtttttgacg
     3541 ccgtatttta cgggaatgat caggcgaagg ggggcaccgt ggttgggggg caggggctgg
     3601 cctgtcattt cgtagcagag gatgg
//
LOCUS       DATGFG010000779         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1384837, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000779 DATGFG010000000
VERSION     DATGFG010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384837"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VKQ52_14575"
     CDS             complement(<1..1058)
                     /locus_tag="VKQ52_14575"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ88476.1"
                     /translation="MRKLLSHLLCLCLAFSQLEAQTPTVPITGSVTDEKGVPLAAVTV
                     TALTGDRKVVTTAVTDANGAFKLNISAKARTLQFSYIGLEEQLVPIGNKNAFVVTLRA
                     SNKNLSEVVVVGYGTQKKTEVTGSIATVKGSAISETPIQSFESGLAGRSAGVQITVPS
                     GVANEPPVFRVRGTNSISLSSYPLIVVDGVPTFTGDFNSPSGTEITSASANPLASINP
                     NDIESIDIAKDPAATAIYGSRAANGVVFITTKKGRPGKPKVTYDGWVGRVKAYGLPKM
                     LNADQYIAFKSQAVANNPALTGPNAVTFNYAKDARGNNVNTNWFDYIYRTGVSHSHNV
                     SISGGNDATTYYLSGGFT"
     gene            complement(1343..1819)
                     /locus_tag="VKQ52_14580"
     CDS             complement(1343..1819)
                     /locus_tag="VKQ52_14580"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ88477.1"
                     /translation="MQKITPFLWFDNQAEEAANHYVSIFKNSKINNITRYGDAGPGPK
                     GSAMMVTFTLEGQDFYALNGGPLFHFTEAISLYVNCETQEEVDTLWNKLLAGGGKESQ
                     CGWLKDKYGLSWQIIPTALRRVLGDKDPARSGRAMQAMLKMQKINIKALEDAFDGK"
     gene            complement(1920..>2156)
                     /locus_tag="VKQ52_14585"
     CDS             complement(1920..>2156)
                     /locus_tag="VKQ52_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88478.1"
                     /translation="EVHASHAKYLSIDGKQIGDPVKAAEALIEIADSPEPPVLLFLGS
                     DAYNRATVKMSELSQLIEKWKSLTLSTQFETVEA"
BASE COUNT          416 a          471 c          687 g          582 t
ORIGIN      
        1 ttagtaaagc cgccggagag ataataggtg gtggcgtcgt tgccgccgga aatgctgacg
       61 ttatggctgt gggagacgcc ggtgcgatag atatagtcga accagttggt gttgacgttg
      121 ttgcccctgg catctttggc ataattaaat gtgacggcat tggggccggt gagcgccggg
      181 ttgttggcga cggcctggga tttgaaggca atatattggt ctgcgttgag cattttgggc
      241 agtccgtagg cctttaccct tcctacccag ccgtcatagg tgactttggg tttgccgggt
      301 cttccttttt ttgtcgtgat gaagacgacg ccgttggcgg cgcggctacc gtagatggcg
      361 gtggcggcgg gatccttggc gatatcgatg ctttcgatgt cgttagggtt gatactcgcc
      421 agggggttgg cggaggcgct ggttatttcc gttccgctgg gactgttgaa gtcgccggtg
      481 aaggtgggga caccgtccac gacgatcaac ggataggagg aaagggagat ggagttggtg
      541 ccccggaccc tgaataccgg cggttcattg gcaacgccgc tgggaacggt aatctgtacc
      601 ccggcgctgc ggcccgcgag gccggattcg aagctttgga tcggggtctc ggagatggcc
      661 gatcctttga ccgtggcgat actaccggtg acctccgttt ttttctgggt accgtagccg
      721 acgacgacga cttcggagag attcttgttg ctggcgcgca gggtaacgac aaaggcgttt
      781 ttattgccga tggggaccag ttgttcttcc agtccgatgt agctgaactg cagggtgcgc
      841 gcttttgcgg aaatattcaa tttaaatgca ccgttggcat cggtaacggc ggtggtgacg
      901 acttttctgt ccccggtgag cgcggtgacg gtgacggccg cgagcgggac gcctttttca
      961 tctgtgacag aaccggtgat gggaacagtt ggtgtttggg cctcgagttg actgaatgcc
     1021 aggcacaggc ataacagatg agacaggagc tttctcattg tgagtgtagt agttttggtt
     1081 ataaatgcga ttcaagttaa cagttgaata agcagggtct ctcttactca accaagacag
     1141 ttcgaacgat aaatttactg cttggcgatt aaaaaaacag attttttttg agaaaatgaa
     1201 attctttccc aaaaattagt gacttccatc aacaataatg ctcatgttat gctccgcaat
     1261 tattttagcg agtgagcagt tccgcgtttt gtttcgcgca gcgagtaagc cgacgcggtt
     1321 ttgcgttgcg tagccggtaa gcttatttgc cgtcgaaggc gtcttccaat gccttgatgt
     1381 ttattttctg catttttagc atggcctgca tggcccggcc ggatcttgcg gggtctttat
     1441 cacccagtac gcggcggagg gcggtgggga tgatctgcca ggagaggccg tatttatcct
     1501 tcagccagcc acactggctt tcctttccgc cgccggcgag gagtttgttc cagagggtat
     1561 ctacttcctc ctgggtctca cagttcacat agagggagat ggcttcggtg aagtggaaga
     1621 gggggccgcc gttgagggca tagaagtcct ggccttcgag ggtgaaggtg accatcatcg
     1681 cggagccttt tgggccgggg ccggcgtcgc cgtagcgggt gatgttgttg atcttcgaat
     1741 ttttgaagat cgagacgtag tggttggcgg cttcttctgc ctgattgtcg aaccaaagga
     1801 agggagtgat cttttgcatg gttagtgatt tttgtgatga tcaaattgac gctacaaaca
     1861 tagggtcaat ttgatcattt gatgttgtgc aaaagcgaca atcccgaggg tcgaatgcgt
     1921 caggcttcga cggtttcgaa ttgtgtcgag agggtcaggg atttccattt ttcgatcagc
     1981 tggctgagtt cgctcatttt tacggtggcc cggttgtagg cgtcgctgcc gaggaagagc
     2041 aggaccggcg gctcgggtga gtcggcgatt tcgatgaggg cttcggccgc ttttaccggg
     2101 tcgccgatct gtttaccgtc gatggacagg tattttgcgt gggaggcgtg tacttc
//
LOCUS       DATGFG010000780         7580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_705134, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000780 DATGFG010000000
VERSION     DATGFG010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705134"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..788
                     /gene="pth"
                     /locus_tag="VKQ52_14590"
     CDS             210..788
                     /gene="pth"
                     /locus_tag="VKQ52_14590"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLZ88479.1"
                     /translation="MGKFLIAGLGNIGAEYAGTRHNIGFDVADALAASLNGKFSVERL
                     AEKVELKWRGHVLVCIKPTTYMNESGRAVKYWMDREKIPISQIIVIVDELALPLSRIR
                     IRPGGSDAGHNGLRSIQETLGSDVYPRLRFGIGNNFPKGKQVDFVLGKWSESELPIVR
                     IKVEKCVEVIESFATIGIERTMTQYNKLEFEL"
     gene            946..1557
                     /locus_tag="VKQ52_14595"
     CDS             946..1557
                     /locus_tag="VKQ52_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLZ88480.1"
                     /translation="MKIFCVGRNYVAHAKELNNEIPEEPIIFMKPKSALLQAHTPFYY
                     PEFTNELHYECELVLRISKNGKYIQERFASKYYDAVTVGVDFTARDIQNELKEKGLPW
                     EKAKAWDNSAAIGKWIPLQNIKNKKDINFCMYKNKELVQQGNSNLMINNFDKIVAYIS
                     NYFSVNIGDLVFTGTPAGVGECVVGDELEGFIEDDSLFTVEIK"
     gene            1601..3667
                     /locus_tag="VKQ52_14600"
     CDS             1601..3667
                     /locus_tag="VKQ52_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLZ88481.1"
                     /translation="MITIDILLKAWRLMCTASEMAATYENNRPLCKYVHSTSRGHEAI
                     QLATAFHLQPCDWISPYYRDESILLGLGFEPLQLMLQLLAKGDDPFTGGREYYSHPNY
                     TGDDRPRMLHQSSATGMQAIPATGLAQGLRYLESIGSPLYRKGPQNESPIVVCSLGDA
                     SVTEGEVSEAFQFAVLKQLPILYLVQDNGWGISVRSDESRAMDAYDYAGGFNGMQRLR
                     IDGSDFLGAYEAMGEVVDSLRNGPGPWLVQARVPLLGHHTSGVRREFYRDQQDMATHT
                     AQDPLPKLRARLLALGVEEDRLTNIEEEARALVARQFQEAVAAPEPDPSTVADHVFAP
                     TPVTEETGNRHPGKGEKVIMVDAALFAIREIMETHPEALLYGQDVGRRLGGVFREAAT
                     LAEQFGDHRVFNTAIQEAYIIGSTVGISAVGALPIVEIQFADYIYPGFNQLVTEISKS
                     CYLSQGRFPVRTLIRIPIGAYGGGGPYHSGSIETTLLSIKGIKIAYPSNAADMKGLMK
                     AAFLDPNPVVMLEHKGLYWSKVPGTEEAKTIEPAADYILPLGKGRCVLAADEHLTAAG
                     DTCCVITYGMGVYWAKTAAARFPGQIEIIDLRTLYPLDEELVYETVCRHGKCLVLTEE
                     QQNNSFAEALAWRITRHCFTALDAPVEVAGALDLPAVPMNAGLESAMLPTPDKVAAIL
                     RNLLQA"
     gene            complement(3685..5151)
                     /gene="moeB"
                     /locus_tag="VKQ52_14605"
     CDS             complement(3685..5151)
                     /gene="moeB"
                     /locus_tag="VKQ52_14605"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HLZ88482.1"
                     /translation="MATVIIPTPLRKFTNNTARLQVGPGTIQDTVRELTQNFPDLKKH
                     LLDEQGRIRSFVNIFIGDDDIRDLQQEHTAVKEDTVISIVPAIAGGSPDGAAATGAVG
                     GARATVFTREELARYNRHIIIPEFGLESQQKLKAAKVLVIGSGGLGSPVLLYLAAAGV
                     GTIGIVDFDVVDDSNLQRQVLFGVDEIGKPKVEAARRRLQALNPYIQLNIYNTHLNSQ
                     NALDILRDYDVIADGTDNFPTRYLVNDASVLLGKPNVYASIFQFEGQVSVFNYRDKQG
                     QLGPNYRDLYPTPPPPGLVPSCAEGGVLGVLPGIIGSLQALEVIKVITGVGDTLSGRF
                     YIFDALNFESRTFRIKPREDNPINGKNPTITALIDYEQFCGMRAVEEKPLKEVTAKEL
                     YDWQVTGEPFQLIDVREPHEYEIVNIGAELIPLATVAANSDRIQRDRKVVVHCKVGGR
                     SAKAIRELEEKFGFTNLYNLKGGILAYIDQIQPELTKY"
     gene            complement(5168..5596)
                     /locus_tag="VKQ52_14610"
     CDS             complement(5168..5596)
                     /locus_tag="VKQ52_14610"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HLZ88483.1"
                     /translation="MIIIEPRVRQLLVEDALNTFPDECCGFLFGREEHDGTRLVVDIL
                     VVDNSKEGDKTRRFEISPQDYMAAETHALDHDWTLLGIYHSHPKHPAIPSEHDRKAAQ
                     PFFSYVIISVMDKDTIHLRSWLLNEEQQFEEEKIIDYIYT"
     gene            complement(5593..6534)
                     /locus_tag="VKQ52_14615"
     CDS             complement(5593..6534)
                     /locus_tag="VKQ52_14615"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HLZ88484.1"
                     /translation="MSTMTLDPANSTLVSAVRGLEKEVGHTPLHHVTGLFRHPRVQVF
                     AKKEWMQLSGSVKARAAYSIIRRAIEEGALTREKTLLDATSGNTGIAYAHIAKRLNIP
                     VALCLPENASRERKEILQSLGVKLILTSKFEGTDGAQQVARSLAEADPDAYFYADQYK
                     NPNNWKAHYHGTAVEIVEALPGITHFVAGLGTTGTFVGTGRRLRELRPGIRLISLQPD
                     LPMHGLEGWKHLETAIVPEIYDPLVADENEEVSTIEAHRLIIDAWQKEGLLLSPSAAA
                     NLAGAIRVASRLEEGIVVTVLPDNADKYSEIIQKLIV"
     gene            6876..7565
                     /gene="rpiA"
                     /locus_tag="VKQ52_14620"
     CDS             6876..7565
                     /gene="rpiA"
                     /locus_tag="VKQ52_14620"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HLZ88485.1"
                     /translation="MDNQAVNGKKIAAGKAIEFIRNGMTLGLGTGSTAYWAIQGIGEM
                     VKQGLSVKAVATSIQSESLARELNIPIIPFSEVDRLDITIDGADEVDEKLNLIKGGGG
                     ALLREKIVAAATRFYIIIVDESKLVRHLGKFPLPVEVVPFGWELTMRRLSELDCTPRM
                     RMAGDKPYFTDNQHYILDCQFGEILDPPALHRKVSDITGVMEDGFFINMADIVVAGSP
                     HGETRIINKPS"
BASE COUNT         1738 a         2194 c         2001 g         1647 t
ORIGIN      
        1 cgcgtcagct gaaacaggaa gaagctgctg cccctgcagc aggtgctaaa gcggcagccg
       61 ctccagctgc ggccgcagcc gctccggcag ctgctaaacc cgcggcaggc aaaaaataaa
      121 tgtttttatt agtaggaacc cggttaaagt ttaaccgggt ttttgttatt tttaacgccc
      181 cccggcggct tggccaaagg gaagacacta tgggaaagtt cctgatcgcc ggcctgggca
      241 acataggagc cgagtatgcc ggcacccgcc acaacatcgg attcgatgtg gcggatgccc
      301 tggcggcaag cctgaatgga aagtttagcg tcgagcgctt agccgagaaa gtggaattga
      361 aatggcgtgg gcatgtcctg gtatgcataa agccgactac ctatatgaat gaaagtggcc
      421 gtgccgtgaa atactggatg gaccgggaga agatacccat ttctcagatc attgtcatcg
      481 tggacgaact ggcgcttcct ctgagcagga tccggatcag gcccggcggc agcgatgccg
      541 ggcacaatgg gttgcgcagc atccaggaga cgctcggcag cgatgtttat ccaaggttga
      601 ggttcggtat cggcaacaat ttcccgaagg gaaaacaagt tgatttcgta cttggcaaat
      661 ggtcggaaag cgaattaccc attgtccgta tcaaggtaga aaaatgtgtc gaagtcattg
      721 aaagctttgc caccattggg attgagcgga ccatgaccca gtacaataaa ttggagtttg
      781 aactatgatt tataaagggg aatctttatt ttcgcataaa tattgcattc atccctaatg
      841 agtaagtatt ttgatattca atatgcctat ggtaagtacc cggggtctct tcaggccaaa
      901 tgaaaactgg attaagaatt ttctatttaa aaaccctgat tgaatatgaa aatattctgt
      961 gttggccgta actatgtggc tcatgccaag gaattgaata acgagattcc cgaggagcct
     1021 atcattttta tgaagcccaa gagcgcactg ctacaagctc ataccccttt ctactatccg
     1081 gaattcacaa acgaactgca ttatgagtgt gaactcgttc ttcgtatatc caagaacggg
     1141 aaatatatcc aggaaagatt cgcttccaaa tactacgatg ccgtcacggt aggggttgat
     1201 ttcactgcac gcgatatcca gaacgaatta aaagagaaag gcctgccctg ggaaaaggca
     1261 aaggcctggg ataactccgc cgccatcgga aaatggatcc cgctgcaaaa catcaagaac
     1321 aagaaggata tcaacttttg catgtataag aacaaggaat tggtccagca gggcaattcc
     1381 aacttaatga tcaataattt cgacaagatc gtagcctata tatccaatta cttctccgtg
     1441 aacatcggcg acctcgtctt taccggcacc cccgcgggcg ttggtgaatg tgtggtaggg
     1501 gacgaactgg aaggctttat cgaagacgat agcttattta ccgttgaaat aaaatagccc
     1561 ggtccatccg ttatcgcgtt gggcgggtcg ccttcttccc atgatcacta tcgatatctt
     1621 gctaaaggca tggcggctga tgtgcacagc ctcggaaatg gccgccacct acgagaataa
     1681 ccgcccgctc tgtaaatacg tccattccac ttcccgcgga cacgaagcca tccaattggc
     1741 taccgccttt cacttacagc cctgtgattg gatcagccct tactaccggg atgaaagcat
     1801 cctgctcggt ctcggcttcg aaccgttgca actgatgttg caactcctcg ccaaaggcga
     1861 tgaccctttc accggcggcc gggaatatta ttcgcacccc aattacaccg gtgacgaccg
     1921 gccccgcatg ctccatcaga gcagcgccac gggaatgcag gcgatacccg ctaccgggct
     1981 cgcacagggg cttcgctatc ttgaaagcat cggctcgccc ctataccgga aaggcccgca
     2041 aaacgaatcc cccatcgtcg tttgctcgct tggcgacgcc agcgtcaccg agggggaagt
     2101 aagcgaggcc ttccagttcg ccgttctcaa acaattaccg attctctacc tcgtccagga
     2161 taatggctgg ggcatcagcg tccgttcgga tgaatcccgg gcgatggatg cctatgacta
     2221 tgcaggtggc ttcaacggaa tgcagcgact acgcattgac gggagcgatt tcctcggggc
     2281 ctatgaagcc atgggcgaag tggtagactc ccttcgcaat ggtccggggc cctggctcgt
     2341 ccaggcccgg gttcccctgc ttggccacca caccagcggc gtccgcaggg aattttatcg
     2401 cgaccagcag gacatggcga cccataccgc gcaggacccg ctccccaaat taagggcccg
     2461 gctgctggcg ctgggggtcg aagaagatcg attgaccaac atagaagagg aagcgagagc
     2521 cctggtggcc cggcaattcc aggaagccgt ggcggcgccc gaacccgacc cgtctaccgt
     2581 tgccgaccac gtgttcgccc ccacacccgt aacggaggag accggcaacc gccatccagg
     2641 gaaaggggag aaggtgatca tggtcgatgc ggcgttgttt gccatccggg aaataatgga
     2701 aacacacccc gaagccctgc tgtatggcca ggacgtcggc cgccggctgg gcggcgtttt
     2761 ccgggaagcg gcaactctgg ccgaacaatt tggcgaccat cgcgtcttca atacagccat
     2821 ccaggaagcc tatatcatcg gttccaccgt tgggatcagc gcggttggcg ccctgccgat
     2881 cgtcgagatc cagtttgccg actatatcta ccccgggttc aaccagttgg tgacggagat
     2941 ttccaagtcc tgttatctta gccagggcag gtttcccgtc cggaccctga tccgtatccc
     3001 catcggcgca tacggcggcg gcggccccta tcatagcggc agcatcgaga caacccttct
     3061 ttccatcaag gggataaaaa tagcctaccc atccaacgcg gcggatatga aggggctcat
     3121 gaaggccgcc ttcctcgacc ccaaccccgt ggtcatgctc gaacacaaag gtctctattg
     3181 gagcaaggtg cccggaaccg aggaggcaaa gaccatcgaa cccgccgcgg attatatcct
     3241 tcccctgggt aaggggagat gcgtactcgc cgccgacgaa cacctcacgg cggcaggcga
     3301 cacctgctgt gtcatcacct atgggatggg tgtatactgg gcaaagaccg ctgcagcccg
     3361 cttcccggga caaatagaga tcatcgatct ccgcaccctt taccccctcg acgaggaact
     3421 ggtgtatgaa accgtatgcc ggcacgggaa atgcctcgta ctgacggagg aacaacagaa
     3481 caattccttt gccgaggcgc tcgcctggcg gatcacccgc cactgcttta ccgcgctgga
     3541 cgcaccggtg gaagtggccg gcgccctcga tcttcccgcc gtgcccatga atgccggcct
     3601 ggagagcgcc atgctcccca cacccgacaa ggtggccgcc atcctgcgaa acctcctgca
     3661 agcctaaact gcgggcggcc ccgatcaata cttcgtcaac tctggttgta tctggtcgat
     3721 atatgcaagg ataccgccct tcaggttata caaattcgta aacccgaatt tctcctccag
     3781 ttcgcggata gccttcgcgc tgcggccacc taccttgcaa tgcacgacga ccttcctgtc
     3841 ccgctggatg cggtcactat tcgcagccac cgtcgccagg gggatcagct ccgccccgat
     3901 gttgacgatc tcgtattcat gcggctcgcg gacatcgatc agctggaagg gctcaccggt
     3961 cacctgccag tcatataact ctttcgcggt gacctctttc aacggctttt cttccaccgc
     4021 ccgcatcccg cagaactgtt cgtagtcgat aagcgcggtg atggtcgggt tcttgccgtt
     4081 gatgggattg tcttcccgcg gctttatccg gaaggttctg ctctcgaaat tcaatgcatc
     4141 gaagatgtaa aaacggcccg acagcgtatc ccccacgccc gtgatgacct tgatcacctc
     4201 cagggcctgc agactgccga tgatgcccgg taataccccg aggacgccgc cttcggcgca
     4261 gctcggcacc agtccgggcg ggggtggagt ggggtagagg tcccgatagt tcggacccag
     4321 ctgcccctgt ttatcccggt aattgaaaac ggacacctgc ccttcgaact ggaagatgga
     4381 ggcatagaca ttcggcttcc ccagcagcac ggaggcatca ttcaccaggt aacgcgtagg
     4441 gaaattgtcc gttccatccg cgatgacatc ataatcccgc aggatatcca gtgcattctg
     4501 ggaattgagg tgcgtattgt agatattcaa ttggatatag ggattcaagg cctggagccg
     4561 gcgtctggcg gcttccacct ttggcttgcc gatctcgtcg acgccgaaca agacctgccg
     4621 ttgcaggttg ctgtcatcga cgacatcaaa atcgacgatc ccgatcgtcc cgaccccggc
     4681 cgccgccagg taaagcagga cgggactccc aagaccgccc gatccgatga ccagcacctt
     4741 tgccgccttc agtttttgct gtgactcgag gccgaattcg gggatgatga tatgccggtt
     4801 gtaccgtgca agctcctccc gggtaaaaac ggtggccctg gcgccaccca ccgccccggt
     4861 cgcggctgcc ccgtccgggc tgccccccgc gatcgccgga acgatactga tcaccgtatc
     4921 ttctttcacg gccgtatgct cctgctgcag atcgcggata tcatcgtcgc cgataaaaat
     4981 attgacgaaa ctgcggatcc tcccctgctc atcgagcaga tgtttcttca ggtcgggaaa
     5041 gttctgcgta agctccctga ctgtgtcctg gatcgtgccc gggccgacct gcagccgggc
     5101 cgtgttattg gtaaatttcc gtaacggagt tggaataata accgttgcca tagtattgat
     5161 gcttttttta agtgtatata taatcaatga tcttttcttc ttcgaattgc tgctcctcgt
     5221 tgagcagcca gctccgcaga tggatggtat ccttgtccat aacggagatg atcacatagg
     5281 aaaaaaaggg ttgcgcggcc ttccggtcgt gctccgaggg aatggccggg tgtttgggat
     5341 gcgaatgata gatccccagc agggtccagt catgatccag cgcgtgggtc tcggctgcca
     5401 tataatcctg cggcgagatc tcgaatcgtc tcgtcttgtc gccttccttc gaattgtcga
     5461 cgacgaggat atcgacaacc agccgcgtgc cgtcatgctc ctccctgcca aaaagaaagc
     5521 cgcagcattc gtccgggaag gtgttgagcg cgtcttcgac taacaattgc ctgaccctgg
     5581 gttcaatgat gatcatacga ttagtttttg gataatttca ctgtatttat cggcattgtc
     5641 gggcagcacg gtcacgacga tgccctcttc caaccggctg gcgacgcgga tagccccggc
     5701 cagattggcg gcggccgacg ggctgagcaa caacccttcc ttttgccagg catcgatgat
     5761 cagccggtgc gcctcgatcg tgctcacctc ttcgttctcg tcggctacca acggatcata
     5821 gatctccggg acgatggccg tctccaggtg cttccacccc tccagcccgt gcataggaag
     5881 atctggttgc agcgagatca gccggatacc gggccgcaat tcccgcagcc gccggcccgt
     5941 acctacaaag gttccagtcg tccccaaccc cgctacaaaa tgggtgatac ccggcagggc
     6001 ctccacgatc tccaccgccg tgccatgata atgtgccttc cagttgttcg ggttcttata
     6061 ttgatctgca taaaaatacg catccggatc ggcttccgcc agcgaccggg ccacctgttg
     6121 cgccccatcg gtgccttcga acttcgacgt caggatcagc ttcaccccca gcgattgcaa
     6181 tatctccttc cgctcccgcg acgcattctc gggaagacaa agcgccacgg ggatgttcaa
     6241 cctcttcgcg atatgggcgt aggcgatgcc ggtattcccg cttgtcgcat ccagcagggt
     6301 cttttcccgg gtaagcgccc cctcttcgat ggcccggcgg atgatcgagt aagcggcccg
     6361 ggcctttaca ctacccgata actgcatcca ttccttcttg gcgaagacct gtacccgggg
     6421 atgccggaac agacccgtca cgtggtgcaa cggggtgtgg ccaacctcct tttccaggcc
     6481 cctgacggcg gaaacaagcg tgctgttggc aggatctaag gtcattgtgg acatagcggc
     6541 aactattaaa atatttcgat taaaacaaaa aagccccact ggtggtggag ctttttaact
     6601 attgttgtta atacagttcg atcagcttat aagaggtcat cctccaccca aataaccgtg
     6661 gcttgccgac aacagcaaca acaacaattc tgatatgcca atacctgggt catgtgaatg
     6721 gaacaatgga taacggaaca aaagtaggga aatttgctgt atttcctacc aatcgactag
     6781 gattttggcc ggccactgcg aaaattaatg atggacggta tcccgatcca ccctattttt
     6841 acacccgaaa atcgcacatt ttaactcctt ttctcatgga caatcaagcc gttaatggta
     6901 aaaagatcgc cgccggaaaa gcgatagaat ttatccggaa tgggatgacc ctgggactgg
     6961 ggacaggttc caccgcctac tgggccatac agggcatcgg tgaaatggtg aagcagggac
     7021 tctcggtaaa ggccgtcgcc acctccatcc agtcggagag cctggcgcgg gaactgaaca
     7081 tcccgatcat acccttctcc gaggtggacc gcctcgacat caccatcgac ggcgccgacg
     7141 aagtggacga aaagctcaac ctgatcaagg gtggtggggg cgcgctgctc cgagaaaaga
     7201 tcgttgccgc cgctacccgc ttctatatca taattgtaga cgaatccaaa ctggtccgcc
     7261 atctcgggaa attccccctt cctgtcgaag tggtcccctt tggctgggag ctgaccatgc
     7321 ggcgcctcag cgaactggac tgtacgccgc ggatgcggat ggccggggac aaaccctact
     7381 tcaccgacaa ccagcactat atcctcgact gccagttcgg cgagatcctc gatccacccg
     7441 ccctgcatag aaaagtcagc gatatcaccg gcgtgatgga ggacggcttc tttatcaata
     7501 tggccgatat cgtcgtcgcc ggctcccccc atggagagac ccgcatcatc aacaaacctt
     7561 cctgatcttc atgaaaatac
//
LOCUS       DATGFG010000781         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_5251, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000781 DATGFG010000000
VERSION     DATGFG010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5251"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VKQ52_14625"
     CDS             <1..492
                     /locus_tag="VKQ52_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88486.1"
                     /translation="PDGMWRMYYKAQAGYSHINVTESKDLYTWSAPKEALKINGEGPI
                     AFQWKGSYWLIIDTWNGQTVHRSADANNWVVQPGGPLLRDGEGTGKDDTPNALHANVV
                     ISNDRAYLYYFTHPGRIGADKSKDTYEQRRTSIQVVELRLNDAGWITADRNTPTYVQL
                     SPP"
     gene            654..726
                     /locus_tag="VKQ52_14630"
     tRNA            654..726
                     /locus_tag="VKQ52_14630"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:687..689,aa:Lys,seq:ctt)
     gene            845..1897
                     /locus_tag="VKQ52_14635"
     CDS             845..1897
                     /locus_tag="VKQ52_14635"
                     /inference="COORDINATES: protein motif:HMM:NF040069.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HLZ88487.1"
                     /translation="MKMPLALIAGVTIALSSCSSTNLMSLSVTEPAPVSLPPTIHSAA
                     VVNRSRATDENRTIDAIHKALSLETRNLQAEGARASVTGLTDELMKNNRFSSVRYLDN
                     LDLRSYGAGVFPTSLPWDSVEKICRESNTDVLFSLELFDAETKVGAGISPTNLTTLAG
                     NIPAIAQQVNVNTLVKTGWRIYDPASRNILDEYILSRDLSFQSRGIDPLAAGSALIGH
                     KEEVKQTAGNAGQAYATRIIPYSIRVSRFYFVRGDANFIIAKRMAQTGNWDGAAKIWQ
                     QETTSSSRKAAGRACYNMAIISEINGDLDGAIQWAQKAYEVYNIRLALSYVNVLRHRQ
                     SDNAVLRSQTEVTSNP"
     gene            complement(1954..>2212)
                     /locus_tag="VKQ52_14640"
     CDS             complement(1954..>2212)
                     /locus_tag="VKQ52_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HLZ88488.1"
                     /translation="VYEPIHGSAHDITGKGVANPLASILSAALLLDISFGMKAESDTV
                     IHAVDQVLKAGFRTRDIANAQTPAAKVLGTEAMGAEVLKFI"
BASE COUNT          547 a          695 c          540 g          430 t
ORIGIN      
        1 ccggacggga tgtggcggat gtactataag gctcaagcgg ggtattcgca tatcaatgtc
       61 actgaaagca aggatcttta cacctggtct gcacccaagg aagcgctgaa gataaacggc
      121 gagggaccga tagcttttca atggaaagga tcttactggc tcattatcga cacctggaat
      181 ggtcagacgg ttcatcgctc ggcagatgca aacaactggg tcgttcagcc tggtggtcct
      241 ctgctgcggg atggcgaggg caccggcaaa gacgataccc ccaatgccct gcacgccaat
      301 gtcgtgatca gcaatgaccg ggcctatctg tattacttta cgcatcccgg ccgtatcggc
      361 gcggataaat ccaaagacac gtacgaacaa cggcgcacgt ccatccaggt ggtggagcta
      421 aggctgaacg atgcgggctg gatcacggcc gacaggaata cgccaaccta tgtgcagctg
      481 tcaccgccat aaagctccaa cggcggtagt ttttcgcccc ctcacctcga cacaaaccgc
      541 accttacccg gacttccgcc cgccctcccc ttctctcaaa aaaaatcccc tcactttgat
      601 ctcgattccc gctttttccc taattttgcc gtccaatctc aaacgagatc tatgtctctg
      661 tagctcagtt ggtagagcag ctgactctta atcagcgggt ccacggttcg agcccgtgcg
      721 gggacactga aaacaaagca cttggcgccc acacggccag gtgctttttt attggatact
      781 aaaaccaccc aatatccgtt gatacatagg aaaaacccat atatccagct aaatccatcc
      841 ccttatgaaa atgcccctcg ccctgatagc aggagttaca atcgccctct cttcctgcag
      901 ttcgaccaac ctcatgtccc ttagcgttac ggaaccggcc ccagtgagcc ttcccccgac
      961 gatccattcg gcagccgtgg tgaacaggag ccgcgccacg gatgagaaca gaaccatcga
     1021 cgccatccat aaagcacttt ccctcgagac cagaaatctg caggccgaag gagccagggc
     1081 cagcgtcacc ggcctcaccg acgaactgat gaagaacaac cggttctcct cggtaagata
     1141 cctcgataac ctcgacctcc ggtcttatgg cgcgggtgta ttccccacct ccctaccctg
     1201 ggacagcgtg gaaaaaatat gcagggaaag caatacggat gtgttgttct cactcgagct
     1261 tttcgatgcc gaaacaaagg tcggcgccgg catttccccg accaatctga caaccctcgc
     1321 gggtaatatc ccggccattg ctcagcaggt gaacgtgaat accctggtaa aaacaggatg
     1381 gaggatctac gaccccgcct cgcgcaatat cctggatgaa tatatccttt ccagggacct
     1441 gtccttccag agccggggta tcgatccgct tgcagcggga tccgccctga tcggccacaa
     1501 agaagaggtg aaacagaccg ccggcaacgc gggccaggcc tacgccacac gcatcatacc
     1561 gtattcaata cgggtatccc ggttttactt tgtacgcggc gacgcgaatt tcatcatcgc
     1621 caaacgcatg gcgcaaaccg gtaactggga cggcgccgca aagatctggc aacaggaaac
     1681 caccagctcc tcacggaaag cagcaggaag agcctgctat aacatggcta ttatcagtga
     1741 gatcaacggc gacctggatg gagccatcca gtgggcccag aaagcatatg aggtctataa
     1801 catccggctg gcccttagtt atgtcaatgt cctgcgacac aggcaatccg ataacgccgt
     1861 cctgaggagc cagaccgaag ttacgagcaa cccctgaccg gcaccccaac cccgagcaac
     1921 agcacccggt ccggcttcga ctgccgaccc ggcctatata aatttcagca cctcggcgcc
     1981 catcgcctcc gtccccagca ccttcgccgc cggcgtctgc gcattcgcga tatcccgggt
     2041 ccggaatccc gctttcaaca cctggtccac cgcatggatc accgtatcgg actccgcctt
     2101 catcccaaaa gagatatcca gcagcaacgc cgcggaaagg atcgaagcaa gcggattggc
     2161 cacccccttc ccggtaatat catgagccga cccgtggata ggttcataca cc
//
LOCUS       DATGFG010000782         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_379921, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000782 DATGFG010000000
VERSION     DATGFG010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379921"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..800)
                     /locus_tag="VKQ52_14645"
     CDS             complement(117..800)
                     /locus_tag="VKQ52_14645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLZ88489.1"
                     /translation="MQLLSRTAIIMTLITVTTLLTSLRTKTQKVIFFGDSITAQGARP
                     GGYIVKMREALTQRGLASDYELTGAGIGGNKIYDLYLRMDDDVLAQNPDVVFIYIGVN
                     DVWHKNTSGTGTDIDKFEKFYTAIINKLLDRHIRTILCTPAVIGEKTDFTNQQDGDLN
                     AYSQVIRNLAQKFHCGLVDLREIFHTYELKNNPGNKDSSILTHDRVHLNDLGNQLVAD
                     KMLEALLAK"
     gene            complement(828..2027)
                     /locus_tag="VKQ52_14650"
     CDS             complement(828..2027)
                     /locus_tag="VKQ52_14650"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HLZ88490.1"
                     /translation="MTAAIYREEMSRELQSILQYWMLNTPDERSGGFIGRITGDDRPH
                     PDSPKGLVLNSRILWAFSAAWRYTGLWIYRPVATRAYDYLVAHFLDREYGGAFWSLDA
                     GGLPLNTRKQIYGLAFTLYGSGEYYRATGDQAVLDQAIDLYHTIEQHSFDPAHGGYFE
                     AFARDWQPLEDLRLSDKDANAQKTMNTHLHLLEAYTTLYAVWPDTGLRGKIRQLLDIF
                     LRHILATATGHCGLFFSNDWQPASGLVSYGHDIEAAWLLHEAALTIEDREWIDRTAAL
                     CLEIADITLEGLDEDGGLWYEKDGDHLIREKHWWPQAEAMVGFLRAWSISGDDRWWER
                     STGVWNFVKKYIRDPNGKEWYWGVTADHRPMPGQDKAGFWKCPYHNSRAAIEIMTTLD
                     RTLEKIP"
     gene            complement(2024..>2595)
                     /locus_tag="VKQ52_14655"
     CDS             complement(2024..>2595)
                     /locus_tag="VKQ52_14655"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HLZ88491.1"
                     /translation="DGKYAFYTRPQDGFINTGNGSGVGFGLSDSITHAVIEKETIIDP
                     RLYHTINEAKNGLGPEPLKTRLGWLHLAHGVRNTAAGLRYTLYLFLTDLQDPTHVRYK
                     PGGYFIAPRGEERVGDVSNVVFCNGWIADENGMVFIYYASSDTRMHVATTTIEQLLDY
                     VVNTPEDQGTSAGSVDTLQRIIDKNMAHQ"
BASE COUNT          477 a          637 c          841 g          640 t
ORIGIN      
        1 taccctttat caaggaatgt ttttacggtg acggcgccca ggccgccgtt tgctccggtg
       61 atcaggatag tctttggcat acaaaagatt tagtgaaatg tagggtaaag gtccggttat
      121 tttgccaaaa gcgcttcgag cattttgtcc gccaccagtt gattgccgag atcgttgaga
      181 tggacccggt catgggtgag aatgctggaa tccttattgc ccgggttgtt ctttagttca
      241 taggtgtgga agatctcgcg cagatcgacg agtccgcagt ggaatttctg ggcgagattg
      301 cggatcacct gggaataggc gttcaggtcg ccgtcctgct ggttggtgaa atcggtcttt
      361 tctccgatga cggcgggagt acaaaggatg gtgcggatat gccggtcgag cagtttgttg
      421 atgatcgcgg tgtagaattt ctcgaatttg tcgatatcgg taccggtgcc ggaggtgttc
      481 ttatgccaga catcgttcac gccgatgtag atgaagacaa catcgggatt ctgggcgagg
      541 acgtcatcat ccatacgcag gtaaaggtca taaatcttgt ttccgccgat gccggcgccg
      601 gtgagctcat agtcggatgc gaggcctcgc tgcgtcaggg cctctctcat ttttacgata
      661 tagccgccgg gtcttgcgcc ctgggccgtt atcgaatccc cgaagaagat gactttttgt
      721 gtttttgttc gcagggatgt caggagagtg gtgacggtga tgagggtcat gatgatagcc
      781 gtacgggaga gcagttgcat tgttttttta tttttcttag gccaaaccta cgggattttt
      841 tccagcgtcc ggtctagtgt tgtcataatt tcgatagctg cacggctgtt gtgatacggg
      901 catttccaga agccggcctt gtcctgaccc ggcatcggcc ggtgatcggc ggttactccc
      961 cagtaccatt cttttccgtt cgggtcccgg atatattttt ttacaaaatt ccagacaccg
     1021 gtggatcgtt cccaccagcg atcatctccg ctgatgctcc aggcccggag gaagccgacc
     1081 atggcctcgg cctgtggcca ccagtgtttt tcccttatca aatggtcgcc gtctttttcg
     1141 taccagaggc cgccgtcttc gtccagccct tccagtgtta tgtccgcaat ttcgaggcaa
     1201 agggcggcgg tcctgtcgat ccattccctg tcttcgatgg taagggcggc ttcatggagc
     1261 agccaggcgg cttcgatatc gtggccatag gagacgaggc cggacgccgg ctgccagtcg
     1321 ttgctgaaga aaaggccgca atgcccggtg gccgtggcga ggatatggcg gaggaagatg
     1381 tcgagaagct gcctgatctt cccgcgcagg cccgtatccg gccatacggc gtacagtgtg
     1441 gtataggcct ccaggaggtg gaggtgggtg ttcatcgtct tttgtgcgtt ggcatccttg
     1501 tcactcaggc gaaggtcttc gagtggctgc cagtcgcggg cgaaggcttc gaagtagccg
     1561 ccgtgggcgg ggtcgaaact atgttgttcg atggtatggt agagatcgat ggcctgatcc
     1621 agtacggcct ggtcgccggt ggcgcgatag tattcgccag atccatagag ggtaaaggcc
     1681 aggccgtaga tctgtttccg ggtgttgagc ggcaggccgc cggcgtcgag gctccagaag
     1741 gcgccgccgt attccctgtc gaggaaatgg gcgaccaggt agtcataggc ccgggtggcc
     1801 acggggcggt agatccatag gcccgtatag cgccaggcgg cgctgaaggc ccagagaatg
     1861 cggctgttga gcacgagtcc ttttggcgag tccgggtggg gcctgtcgtc gccggttatg
     1921 cgcccgataa agccgcccga tctttcgtcc ggggtattga gcatccagta ctggaggatg
     1981 gattgcaatt cccggctcat ttcttcccgg tagatggcgg ctgtcattgg tgggccatgt
     2041 tcttatcgat gatgcgctgg agggtgtcga cggagccggc cgatgtcccc tgatcttcgg
     2101 gggtattgac gacatagtcc agcagttgtt cgatggtggt ggtagcgacg tgcatccggg
     2161 tatcggaaga agcgtagtag ataaagacca tcccgttctc gtcggcgatc cagccgttgc
     2221 agaagacgac gttggagacg tcccctaccc tttcttctcc tcttggggcg atgaagtagc
     2281 caccgggttt atagcgtacg tgggtggggt cctgcaggtc ggtgaggaag agatagaggg
     2341 tatagcggag gcccgcggcg gtgttgcgga ccccgtgggc gagatggagc cagccgaggc
     2401 gggtctttag cggttcgggg cccaggccgt tctttgcctc attgatggta tgatagagcc
     2461 gggggtcgat gatggtctcc ttttcgatga ccgcgtgggt aatggaatcg gacaggccaa
     2521 agccaacgcc gctgccgttg ccggtgttga taaagccatc ctgcggccgg gtgtagaagg
     2581 cgtatttccc atcga
//
LOCUS       DATGFG010000783         4878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_858824, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000783 DATGFG010000000
VERSION     DATGFG010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858824"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..687)
                     /gene="rplE"
                     /locus_tag="VKQ52_14660"
     CDS             complement(118..687)
                     /gene="rplE"
                     /locus_tag="VKQ52_14660"
                     /inference="COORDINATES: protein motif:HMM:NF000585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HLZ88492.1"
                     /translation="MSTVKYTPRLADKYKKEVVPALMKKFGYSSIMQAPKLEKICINR
                     GVNGAVTDKKLVEVAVEELTTITGQKAVTTQSKKDISNFKLRKNMPIGARVTLRGEKM
                     FEFLDRLVSTALPRVRDFKGINEKSFDGRGNYTLGITEQIIFPEIDIDKVNKITGMDI
                     TFVTTANTNEEAYELLKELGMPFRNIKKD"
     gene            complement(684..1022)
                     /gene="rplX"
                     /locus_tag="VKQ52_14665"
     CDS             complement(684..1022)
                     /gene="rplX"
                     /locus_tag="VKQ52_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HLZ88493.1"
                     /translation="MSNRFKPKFNIKKGDSVVVITGDDKDPKKARKVLEVYPDEAKVL
                     VEGVNIVTKHTKPSAQNTKGGIVKKEAPIHISNIQLFDAKTGGATKVKRSRENGKLVR
                     IAKKSGEVIK"
     gene            complement(1057..1425)
                     /gene="rplN"
                     /locus_tag="VKQ52_14670"
     CDS             complement(1057..1425)
                     /gene="rplN"
                     /locus_tag="VKQ52_14670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HLZ88494.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGNSGQDYAGIGDKIVVTVK
                     DALPSGGIKKGTVAKAVIVRTKKKLRRKDGSYISFDDNAVVLLNASDEPRGTRIFGPV
                     ARELRDKGYMKIISLAPEVL"
     gene            complement(1541..1798)
                     /gene="rpsQ"
                     /locus_tag="VKQ52_14675"
     CDS             complement(1541..1798)
                     /gene="rpsQ"
                     /locus_tag="VKQ52_14675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HLZ88495.1"
                     /translation="MVERNLRKTRIGVVKSNKMDKTITVAVERKVKHPIYGKFVKKTT
                     SFHAHDEKNECTVGDVVRIMESRPLSKTKRWRLVEVVEKAK"
     gene            complement(1910..2122)
                     /gene="rpmC"
                     /locus_tag="VKQ52_14680"
     CDS             complement(1910..2122)
                     /gene="rpmC"
                     /locus_tag="VKQ52_14680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HLZ88496.1"
                     /translation="MSKRIDFINSLKDLNENDLKARIQEDEQRLKKLEFAHAISPLEN
                     PMSIRSLRKDVARLKTALAQKETQGA"
     gene            complement(2141..2578)
                     /gene="rplP"
                     /locus_tag="VKQ52_14685"
     CDS             complement(2141..2578)
                     /gene="rplP"
                     /locus_tag="VKQ52_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HLZ88497.1"
                     /translation="MLQPKRTKHRKAQKGRIRETAKRGSSIAFGSYALKSLEAIWLTN
                     RQLEAARQALTRAMKREGNVWIRVFPDKPITKKPLEVRMGKGKGNPEYWAAVVEPGRI
                     LFEADGVPLAVAKEALELAAQKLPIKTKFIVRRDVEAEVQIKK"
     gene            complement(2633..3484)
                     /gene="rpsC"
                     /locus_tag="VKQ52_14690"
     CDS             complement(2633..3484)
                     /gene="rpsC"
                     /locus_tag="VKQ52_14690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HLZ88498.1"
                     /translation="MGQKTNPIGARLGIIRGWESNWYGSKKDYATKLIEDNKIRTYLN
                     ARINKGGIAKIVIERTLNKLIVTIHTSKPGIIIGKGGGEVDRIKEELKKLTGKDDVQI
                     NILEIRRPELDANIVGDTIARQIENRINYKRAIKMAIASALRMGAEGIKIKVAGRLGG
                     AEIARTEEIKQGRTPLHTLRMDIDYANVFALTVYGKIGIKVWICKGEVLAKRDLNPNF
                     VGGKEGGLSDRRDRRDGDHDHGHRGDRRGGDRGERRGGGDRGGDRRHGGGDRGGDRRP
                     GGGGGRR"
     gene            complement(3512..3868)
                     /gene="rplV"
                     /locus_tag="VKQ52_14695"
     CDS             complement(3512..3868)
                     /gene="rplV"
                     /locus_tag="VKQ52_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HLZ88499.1"
                     /translation="MEAVAKLRNYPTSPRKMRLLADEIRGMHVEKALALLEHHPQHSS
                     TPLHKLLWSAVKNWEQKNEGQSAADAGLVVKTVFVDGGRTLKRMRPAPQGRGYRVRKR
                     SNHVTIIVDSKVVAQN"
     gene            complement(3879..4151)
                     /gene="rpsS"
                     /locus_tag="VKQ52_14700"
     CDS             complement(3879..4151)
                     /gene="rpsS"
                     /locus_tag="VKQ52_14700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HLZ88500.1"
                     /translation="MGRSIKKGPYVDSNLEEKILAINEGTAKKGVLKTWSRRSTITPD
                     FVGHTLAVHNGNKFIPVYVTEFMVGHKLGEFAPTRNFKGHSSKKIA"
     gene            complement(4166..>4878)
                     /gene="rplB"
                     /locus_tag="VKQ52_14705"
     CDS             complement(4166..>4878)
                     /gene="rplB"
                     /locus_tag="VKQ52_14705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HLZ88501.1"
                     /translation="NAQGRRAMRYMGGGHKKMYRVIDFKRNKKGISATVASIEYDPNR
                     TAFIALLNYTDGEKRYILAPQGLQVGATVLAGDGVAPEVGNALQMKNMPLGTNVHNIE
                     MQPGQGGKLVRSAGTSAQLTNKEEKYAVLKMPSGELRKVLINCYATVGVVSNSDHSLQ
                     SMGKAGRNRWRGIRPRNRGVAMNPVDHPMGGGEGKASGGQPRSRNGQYSRGLKTRQKG
                     KGSDNLIIQRKNGKKLSK"
BASE COUNT          965 a         1451 c         1100 g         1362 t
ORIGIN      
        1 ataatcgccg gcggctttta aagcggcgcg tttctcggca tactttgccg acaacacttc
       61 cctttttctt tgccttgcct ttacggactc tttagccatg ttatgttctt ttaaagttta
      121 atctttctta atgttacgga aaggcatgcc gagttccttc agcagctcgt aagcctcttc
      181 gttggtgttg gcagtggtca cgaaagtgat atccataccc gtgatcttgt tcaccttgtc
      241 gatatcgatc tccgggaaga tgatctgctc cgtgataccc agggtgtagt tgccgcggcc
      301 gtcaaaggat ttctcgttga tccccttgaa gtcacgaacc cggggcagcg ccgtggaaac
      361 caggcggtcc aggaattcga acatcttctc cccacgcagc gtaacccgcg caccgatggg
      421 catgttctta cgcagcttga agttggagat atccttcttc gactgcgtgg tcacagcctt
      481 ctgaccggtg atcgtcgtca gctcttccac agctacttcc accagttttt tgtcagtaac
      541 cgcaccattg acgccgcggt taatgcaaat tttttccagc ttcggcgcct gcatgatgct
      601 gctgtagcca aacttcttca taagggcagg tacaacttcc ttcttgtact tgtctgccag
      661 acgcggagtg tattttacag tactcatttg attacctccc cagatttttt agcgattcga
      721 actaatttcc cgttctcccg gctgcgcttt accttggtag cgccgcccgt cttcgcatca
      781 aacaactgga tgttggagat gtgaatggga gcttccttct tcacaatgcc acccttggtg
      841 ttctgtgccg aaggtttggt gtgcttggta acaatgttca ccccctcgac caaaaccttg
      901 gcctcgtcgg gataaacttc caggactttc cgggccttct tcgggtcctt gtcgtctccg
      961 gtgatgacta ccacgctatc acctttttta atattgaact tgggtttgaa tctgttgctc
     1021 attgttttat atttttaacg tccttacagc cggtccttaa agcacctcgg gcgcaaggga
     1081 gatgatcttc atgtaacctt tatcacgcaa ctccctggcg acaggcccga agatgcgggt
     1141 accgcggggt tcgtcggatg cgttcagcaa taccaccgca ttgtcatcaa agctgatata
     1201 gctgccgtcc ttgcggcgta acttcttctt ggtgcgaacg atcaccgcct tggcgacggt
     1261 ccccttcttg ataccgccgg aaggtaacgc atccttcacg gtgactacga tcttgtcgcc
     1321 gatgcccgcg tagtcctgcc cgctgttacc cagtacccgg atgcacagca cttccttggc
     1381 gccgctgtta tcagccacat ttaatctgct ttcttgctga atcattttat tagcttttat
     1441 gtgcgagagt atgtttttct ccgaaattta acccatcctc tcgcacctct tatgatgggc
     1501 cttcggccag cggacccgtg gtccgctttg ctgtatgaac ttatttagcc ttctccacta
     1561 cctcaacaag gcgccaacgc ttcgttttgc tcaacgggcg gctttccatg atccggacga
     1621 cgtcaccgac ggtgcactcg ttcttctcgt catgggcgtg gaaactggta gtcttcttta
     1681 cgaacttacc atagatcggg tgcttcactt tcctttcaac agccacggtg atggtcttat
     1741 ccatcttgtt gctcttcaca acaccgatcc tcgtcttgcg caaatttctt tcaaccattg
     1801 tattagcttt ttgcattatt aactatgctt tgaccttcgt tcgccgcgcg acccgaaaac
     1861 atgcgcgcct ctctccggtc atcttatgtc tgcccttcgg gcgcggggcc tatgcccctt
     1921 gggtctcctt ctgggccaac gccgtcttca gacgggcaac atcttttctc aaagagcgga
     1981 tgctcatcgg gttctccagc ggagaaatgg catgcgcaaa ttccagcttc ttcaggcgct
     2041 gctcgtcttc ctggatacgg gccttcagat cattctcgtt gagatccttc aggctgttta
     2101 taaaatcgat tctctttgac atcgtatatc ttttttgcga ttatttcttt atttgtacct
     2161 cagcctcaac atcccggcga acgatgaact tcgtcttgat cggcagcttc tgcgcagcca
     2221 gttccagcgc ttccttcgca acggccaggg gcacaccatc cgcttcgaac aggatacgac
     2281 ccggctcgac aacagcagcc cagtactcgg ggttaccctt accctttccc atacgcactt
     2341 caagcggctt cttggtgatc ggcttgtcag ggaaaacccg gatccacaca ttaccttcac
     2401 gcttcatggc gcgggtcaga gcctgacggg cagcttccag ttggcgattg gtcagccaga
     2461 tcgcttccag cgatttcaaa gcatacgaac caaaagcaat agagcttcct ctttttgccg
     2521 tctcacggat gcgacctttc tgtgctttcc tatgcttggt tcttttaggt tgtaacatct
     2581 tagaattatt ttctggtact agtttctttt attacttaat gtcctccggc gcctatcgac
     2641 ggccaccgcc accacctggc ctgcggtctc caccacgatc tccgccgcca tgcctgcggt
     2701 caccaccccg gtctccaccg ccacgacgct cgccacggtc gccaccacga cgatcacccc
     2761 gatgaccatg atcatggtcg ccatcccttc tgtcccggcg atcgctcagg ccaccttctt
     2821 tcccaccgac aaagttgggg ttcaggtctc ttttcgcaag cacctcaccc ttacagatcc
     2881 agaccttgat cccgatcttc ccgtagacgg tgagcgcgaa cacattcgca tagtcgatat
     2941 ccatccgcaa cgtatgcagc ggcgtacgac cctgcttgat ctcctccgta cgggcgatct
     3001 cggcgccgcc cagccggccg gcaaccttga tcttgatccc ttctgcaccc atcctgagcg
     3061 cggaagcgat cgccatcttg atagcacgct tatagttgat acgattctcg atctggcggg
     3121 caatggtgtc accaacgatg ttggcgtcca attccgggcg acggatctcc aggatattga
     3181 tctggacatc atccttgcct gtcagcttct tcaattcttc cttgatgcga tcgacctcgc
     3241 cgccaccctt gccgataatg ataccgggct tggaagtgtg gatcgttacg atgagtttat
     3301 tcagcgtacg ctcgatcacg atcttggcaa tccccccctt attgatacgg gcattcaggt
     3361 aggttctgat cttgttatcc tcgatcaact tggtagcata atcttttttg ctgccatacc
     3421 agttggattc ccaaccgcgg atgatcccta acctggcccc aataggattc gttttctgac
     3481 ccatattata gcttaataaa ttttatctaa attagttttg agcaacgact tttgaatcaa
     3541 cgataatggt tacgtggttg cttctcttgc ggacacgata acccctgccc tgaggggccg
     3601 ggcgcatacg cttcaacgtc cttccaccgt ccacgaaaac cgtttttacc accagtccgg
     3661 catcggctgc actctgccct tcgttcttct gctcccagtt cttcacagcg ctccacaaca
     3721 gcttatgaag cggggtggaa gaatgttgcg gatgatgctc cagcaacgcc agggctttct
     3781 caacatgcat cccgcggatc tcatcagcca gtaaacgcat cttacgcggc gacgtcgggt
     3841 aatttctcaa tttagctact gcttccatta taataagttt aagcgatctt tttgctcgaa
     3901 tgtcctttaa agttgcgggt gggtgcaaac tcaccaagct tgtgacctac catgaactcc
     3961 gttacataca ccgggatgaa tttgttaccg ttgtgcacag ccagcgtatg acccacgaag
     4021 tcgggggtga tcgtactccg gcggctccag gttttaagaa cccctttctt ggctgtccct
     4081 tcattgatag ccaggatctt ctcttccagg ttggagtcta cgtaaggacc ttttttaatt
     4141 gaacgaccca tgattgatag ttaaattatt tcgacaattt tttaccgttc ttccgttgga
     4201 tgatcaggtt atcactgccc ttgcccttct gacgggtctt caggccgcgg ctgtactgac
     4261 cgtttctgct gcggggctgg ccaccggatg ccttaccctc accaccaccc atcgggtgat
     4321 ccaccgggtt catcgctaca ccacggttgc gcggacggat acccctccaa cgattgcgac
     4381 cggccttacc catgctctgc agggagtggt cactgttgga taccacccca acggtagcat
     4441 aacagttgat caacacctta cgcaattcgc cggaaggcat cttcaacacg gcatatttct
     4501 cttccttgtt ggtcagctgg gcggaagtac cggcgctgcg caccagcttg ccaccctgac
     4561 caggctgcat ttcgatattg tgtacattgg tacccagcgg catgttcttc atctgcaggg
     4621 cattcccaac ttcaggagca acaccgtcac cggccagcac cgtagcgcca acctgcaaac
     4681 cctggggagc caggatatac cgcttctcac catccgtata attaagcagg gcgataaagg
     4741 ccgtacggtt cggatcatac tcgatcgacg ccaccgtggc agagatcccc ttcttgttac
     4801 gcttgaaatc gatcacccgg tacatcttct tgtgaccgcc gcccatataa cgcatcgccc
     4861 tgcggccttg cgcattcc
//
LOCUS       DATGFG010000784         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1590241, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000784 DATGFG010000000
VERSION     DATGFG010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590241"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..2535
                     /locus_tag="VKQ52_14710"
     CDS             193..2535
                     /locus_tag="VKQ52_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ88502.1"
                     /translation="MKRFIGLLLLVCSRPVLAQHHYTLLVLHDETRQPLPGVTIRIQP
                     VNATVVSDSTGRAFLPALGPGNYTLQCTCIGFREREVALKIPLRVSLPLPVSPPVKAA
                     VKLPLKVASELEPKTAAELTLKAAPELEPKAAAGGQDSSLTVTLEPMVETMQDVVVSS
                     TRTNQLVKNTPLAVQVIDREDIEEGTAQSPANIRELLTELSGTQMQQTSAVSGNVSIR
                     LQGLDGRYTQLLKDGFPLYGGFSGSLSILQIPPLDLRQVEVIKGAGSALYGGDAIAGI
                     INLISRSPDNALHLDAVGNQTNRGGTDLAGFYSQRGKRMGVTVLATGSRQQPMDINND
                     GFTDVPRVRQGTVAPTLFWYPDDSTTVRLGAAVSTENRSGGDLRAVEHGADSLHPFLQ
                     QNHSDRSYYQLSLVHTASGRQQFSLKNSVGYFYRSIRQSSGGAPGEPGGIGVSGFSGG
                     EVSSFTEASYAVGHGPHQIVGGVSFVTDAFRPDAADETLGYTHHTVGAFVQDDWQLGK
                     KTVLEAGARGDVEHSFYFLPRLALLYRPIPALSARAGGGLAYKLPTVFNATDEEEAYR
                     QVYPIAPSVQAERSASVNVSLNYQGRIGDDIGLVMDQNFYYTRLAHALIPDGDSLQRG
                     WLYYRNAPGPVVSRGWETNARFTLEDLALYLGYTYTDARRLYLPGSPALPLTPRNRIV
                     SSLTYEAEPHWKAGVEGFYTDGQLLDDGRRAPAFWTFDLMVQRSWGHCSLLLNLENFT
                     DTRQSRFGALYSGTLQAPVFNEVYAPMEGRVVSVALRYRL"
     gene            complement(2630..>4460)
                     /locus_tag="VKQ52_14715"
     CDS             complement(2630..>4460)
                     /locus_tag="VKQ52_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88503.1"
                     /translation="WQGGVASAPSKNDLVDVYAHMRRDGTSVYDSLWFFTGIVAYGNA
                     ANSYYDVELYKKSFGYNSSNGIFTSFGTSGGHSEWLFDAVGNIIQTGDMIVGVSFMPG
                     SVPVIDVRIWVSQTTFASYYGGARVPTYFNFNGNYSSTMGTYGYASIVSKAGTTAFGG
                     GIANYSGTPANDTTYATPWGTSNSSSGWSPNYQASQFIEVGLNLTRMGVDPALYSTLN
                     PCQSFFSNIFFKSRSSASFTANLQDFVAPLTFLRSPVMDFGIKGDTLRCNHTTGSITL
                     TDTTTAAYYTWKTTGGGAITGANSDSSQLSISKPGSYIVSASPALGCPATQTDTIVVP
                     IDTFPPVASAFSGMFNNHIYLYGGNVAASNYSTPFGGSQGLNWNWTGPNSFTSTVQNP
                     VTDTAWGTYDHTVTEKRNGCTDTASTLVLASMFTVLQTDQLQLRGTYTGQQVDLIWQD
                     APRPGTEYIVERSDGRSGFLPIGTVTVPASEQPGSSATFTFADTRPVEGNLLYRIKAL
                     STDGSIYYSPTLAIGIGSSSLLSAWLAANEPAAPTLVARSTRDITGTLVEYNSAGQVL
                     TRKILSFSSGLNTIPLNDIPKGSVRVVALYVDNRLVWCQKAQY"
BASE COUNT          841 a         1156 c         1515 g          948 t
ORIGIN      
        1 ctgtcttttc tcctacgcct gcatattttc cgcaacggcc atcggtggcc tctcctatcc
       61 aacgatggct ggtgttttgt tcgctgcgca tttgatgtat tgccggctcc cgagcggctc
      121 gcccggtaag aggcctgttc cttttttgtt tgactgcctt ccttcctttt tacatcaaat
      181 gctctttttt ccatgaagcg atttatcggg ctgctcctgc ttgtatgcag caggcctgtt
      241 ctggcccagc accattatac tctacttgtt cttcatgatg agacccggca accccttccc
      301 ggggtgacga tccgtatcca gcccgtcaat gccaccgtcg tgtcggattc caccggcagg
      361 gcttttcttc ccgcccttgg cccgggcaat tatacattac aatgcacctg tatcggattc
      421 agagagcggg aggtggcgct gaagatcccg ctgagggtgt cccttccctt gccggtgagt
      481 ccgccggtga aggcagcggt gaagttgccg ttgaaggtgg cgtcggagtt ggagccgaag
      541 acggcggcgg agttgacgtt gaaggcagcg ccggagttgg agccgaaggc ggcggcgggc
      601 ggacaggaca gcagtcttac cgtgaccctc gaaccaatgg tggagacgat gcaggatgtc
      661 gtcgtctcct cgacgcggac caatcaatta gtaaagaaca ccccgcttgc cgtgcaggtg
      721 atcgaccggg aagatatcga agaaggcacc gcacagtcgc ccgccaatat ccgtgagctg
      781 ctgacggagc tttccgggac gcagatgcag cagacctcgg cggtgtcggg gaatgtgagc
      841 atccgattgc aagggctcga cggtcgatac acccaactgc tcaaggacgg gtttcccttg
      901 tatggcggct tttcgggcag cctgagtatt ttgcagatac cgccgctgga cctccggcaa
      961 gtggaagtga tcaagggggc cggctcggcc ttatacggcg gcgacgccat cgcggggatc
     1021 atcaacctga tcagccggag cccggataac gctctccacc tggatgcggt ggggaatcag
     1081 accaaccggg gcggtaccga cctggccggc ttttacagcc aacgcggcaa gcggatggga
     1141 gtcaccgtcc tggcgacggg cagtcgtcag caacccatgg acatcaataa tgatggcttt
     1201 accgatgtgc cacgggtgag gcaggggacc gtggccccta cccttttctg gtatccggat
     1261 gacagcacga cggtgagact cggagcggcg gtctccaccg agaaccggag cggcggggac
     1321 ctgcgggcag tggagcatgg ggccgacagt ctgcatcctt ttcttcagca gaaccatagc
     1381 gaccggagct attatcagct gtcgctggtg cataccgcat ccgggcggca gcaattttcg
     1441 ctgaagaatt cggtgggtta tttttaccgg agcatccggc agagcagtgg tggggcgcct
     1501 ggtgaacccg gcgggatcgg cgtttcgggt ttctccgggg gcgaggtctc ttcttttacc
     1561 gaggccagct atgccgttgg ccatggcccc catcagatcg tcggcggggt gagttttgtt
     1621 acggatgctt tccggcccga tgccgctgat gaaaccctgg gatacaccca ccatacggtc
     1681 ggcgcatttg tacaggatga ctggcagttg ggaaagaaga cggtgctcga agcgggagcc
     1741 cgtggggatg tcgagcatag tttttatttt ttgccccggc ttgccctgct ttaccggcct
     1801 atcccggcgc ttagcgcccg ggcgggcggc gggcttgcgt ataagctgcc cactgttttc
     1861 aacgccacgg atgaagaaga ggcctaccgg caggtgtacc cgatcgcgcc gagcgtacag
     1921 gcagaacggt cggccagtgt caacgtctcg ctcaactacc agggccggat cggggatgat
     1981 atcgggctgg tgatggatca gaacttttat tatacccggc ttgcacatgc gttgatcccg
     2041 gatggcgaca gcctgcaacg gggctggctt tattatcgga atgcgccggg accggttgtg
     2101 tcgcgcgggt gggagacgaa tgcgcggttc acactggagg atcttgcgct ttacctggga
     2161 tatacttata ccgatgcgcg gcggctttac ctgccgggaa gcccggcttt gccgctgacg
     2221 ccgaggaacc ggattgtcag cagcctcacg tatgaggcgg agccgcattg gaaggcgggg
     2281 gtggaaggtt tttataccga tgggcagtta cttgacgacg ggcggcgggc tcccgccttc
     2341 tggaccttcg acctgatggt gcagcggagt tggggccatt gctcgctgct actgaacctg
     2401 gagaatttta cggatacacg gcagagccgg ttcggggcgc tgtacagcgg cacgctacaa
     2461 gccccggtgt tcaacgaggt gtatgcgccg atggaggggc gggtcgtgag tgtggcgttg
     2521 cggtaccggc tgtgaaccgg tgacgaacgc tgttaaccga cgacaaaaag ggccggagtt
     2581 tttccggagg ttcggcattt cgaggcgccg gaggttagta tcgggccttt taatactggg
     2641 ccttctggca ccagaccagc cggttgtcga cgtacagggc gaccacgcgg acgctgcctt
     2701 tcgggatgtc gttcagcggg atcgtattga ggcccgacga gaatgacagg atctttcgcg
     2761 tgaggacctg accggcggag ttgtattcta ccagtgtccc ggtgatgtcg cgggtggatc
     2821 gggcgaccag tgtgggcgct gccggttcgt tggctgccag ccaggcggac aagagagacg
     2881 aggagcctat gccgatggcc agcgtgggag aataatagat gcttccgtca gtggacaggg
     2941 ctttgatccg atagagaagg ttaccttcca ccggccgggt gtcggcaaag gtgaaggtag
     3001 cggaactgcc gggttgttcc gaggccggaa cggtgaccgt gccgatgggc aggaagccgg
     3061 accgtccgtc gctacgctcg acgatgtatt ccgtgccggg gcggggcgca tcctgccaga
     3121 tgaggtctac ctgttggccg gtgtaggtgc cgcgcaactg cagttgatcg gtttgcaaga
     3181 cggtgaacat ggaggcaagg acgagggtgg aagccgtgtc cgtacagcca ttgcgttttt
     3241 cggtgacggt gtggtcatat gttccccagg cggtgtcggt gacgggattc tggacggtgg
     3301 aggtgaagct gttcggtccg gtccagttcc agttgaggcc ctgtgagccg ccaaagggag
     3361 tggaatagtt gcttgcggcg acatttccgc cataaaggta gatgtggttg ttgaacatgc
     3421 cggaaaatgc gctggccaca ggcggaaagg tatcgatggg aacgacgatg gtatccgtct
     3481 gggtagcagg gcagcccagt gcgggggagg ccgacacgat ataggagccg ggtttggaga
     3541 tgctgagctg ggagctatcg ctgttggcac cggtgatggc gccgccgccg gtcgttttcc
     3601 aggtgtagta ggcggcggtg gtggtgtcgg tgagcgtgat cgacccggtg gtatggttac
     3661 accgcagcgt atcccccttg atgccgaagt ccatgacagg cgaccgaagg aaggtgagcg
     3721 gggcgacgaa gtcctggagg ttggccgtga aggaagccga ggaccgggat ttaaaaaaga
     3781 tgttggagaa gaaggactgg caagggttga gcgtcgaata gagggcggga tccacgccca
     3841 tacgggtgag gttcaggcct acttcgatga actgcgaagc ctggtaatta gggctccagc
     3901 ctgaggagct gttggaagtg ccccaggggg tggcataggt ggtgtcgttg gcgggcgttc
     3961 ctgaatagtt ggcgatgcct ccgccgaatg ccgtggtgcc ggccttgctg acgatggacg
     4021 cgtatccgta ggttcccatg gtgctgctgt agttgccgtt gaaattgaaa taggtgggaa
     4081 ccctcgcacc gccgtaatac gaggcaaagg tggtctggct gacccagatg cggacatcga
     4141 ttacgggtac cgagccgggc atgaagctga cgccgacgat catgtcgccc gtctggatga
     4201 tattgccgac ggcgtcgaag agccattcgc tgtggccgcc gctggtcccg aaggaggtga
     4261 atattccgtt ggatgaatta tagccaaagc ttttcttgta taactctacg tcataatagc
     4321 tgttagcggc gttgccgtag gcgacgatgc cggtgaagaa ccaaagggag tcatagaccg
     4381 atgtgccgtc gcggcgcata tgggcataga cgtcgacgag gtcgttcttg ctcggggcag
     4441 aggccacacc gccctgccag
//
LOCUS       DATGFG010000785         3934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1271428, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000785 DATGFG010000000
VERSION     DATGFG010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271428"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VKQ52_14720"
     CDS             <1..647
                     /locus_tag="VKQ52_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88504.1"
                     /translation="QKVYYNYAGSPQYTTDAAGTTKYVSPTGGTSITQAADGVMYKNA
                     LPRYVGGWTNSFAYKGFSLDVLLTYQAGFSVYYGTNAGLHDQRFWNNTTDVLTDAWSA
                     KSANNAKYAKPIFGDNVSNGSAMPMDINVFKGDFIKLRNITLSYSVPPSMMQRIGLSS
                     ARVYISGQNLGIMTKYPGPDPEVSSNGNSNGGQGVDRNTVPNARTFTVGLNVGF"
     gene            684..2165
                     /locus_tag="VKQ52_14725"
     CDS             684..2165
                     /locus_tag="VKQ52_14725"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88505.1"
                     /translation="MKKYNQYIAPFLLGLMALGSCQKKDLYPVPQTAVSDASAFSTAG
                     RIQSQVLSLYGALKAGGFYGGRYVIDGDIKADNFINEQSNLVTGADVWNENPTNSATA
                     VTGLWSAAYLTINACNLFIDGMNAGGTKVVGPTAAGNYISEAKLIRALSYYSLLQYYA
                     LPYANNNGANKGLPLRLTGIKSGGFSLLARSPVDSIYTQILSDLNDAEAGLPLSNGSA
                     YNNVTRAHRNTAIALKTRVYLSMQNYNAVITEANKIVGPSAPFTSPSGVTFALQSDIT
                     KVFVAPYTTSESILSLPMSSTTGDNPGTQNQLGFYFSPSKGAVSGGTGNGEYSLNPAG
                     VIADPKWTATDRRRTFIVTSTTGGKKWLNKYPAPSPYTDFPIVIRYSEVLLSLAEARV
                     RSTNSIDAQAVALLNAVRSRSDATTTYTVASFAGTSDLINAILQERNIEFLGEGLRNN
                     DLMRLQLTVPAKGSAPAKSPSDVGYIWPISATELSLNTLCTDN"
     gene            complement(2252..3052)
                     /locus_tag="VKQ52_14730"
     CDS             complement(2252..3052)
                     /locus_tag="VKQ52_14730"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HLZ88506.1"
                     /translation="MKTGRKLLLAAGLAGVAMIAGAFVYKGHGLATGKGDVLFGMAPA
                     AKGFAVVELFTSEGCSSCPAADALVATVQKEDKDLPVYILSFHVDYWDRLGWKDAFSD
                     AAYSDRQREYARWLRLSSVYTPQVVVNGRKEFVGSDAGSLRRTISSSLQEGAVAGVSL
                     SGVRLSGGRLTWQAKIEGAGSRTSLVVAVVEHSAVTSVKAGENGGRTLSHVQIVRGLE
                     TQPSDGKGNASGHLDWPAGVNAADGEVIAFLQDQDSGKIIAATRSVVE"
     gene            3197..3586
                     /locus_tag="VKQ52_14735"
     CDS             3197..3586
                     /locus_tag="VKQ52_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ88507.1"
                     /translation="MLVYTRAFSSYSVDDLAKAKEFYEKILGFPVTQTPEGLSMQVTK
                     DYSVFLYPKPNHEPATFTVLNLQVMSVDAAVDALTAAGVKFEQYEGEIKTDEKGIFHG
                     GPGKGPTIAWFADPAGNILSVVENPPA"
     gene            complement(3620..>3934)
                     /locus_tag="VKQ52_14740"
     CDS             complement(3620..>3934)
                     /locus_tag="VKQ52_14740"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88508.1"
                     /translation="GRMKPGAARAMEGLLAEARLELAEARGAFFRALDHSWEAVEKGA
                     ASPALFGEVGRVSHALALAVREWVDRLYPYAGLGAARVDTEINRVWRDLHTAGQHPLL
                     VF"
BASE COUNT          921 a         1420 c          876 g          717 t
ORIGIN      
        1 atcagaaagt gtactacaac tatgccggta gcccacaata caccactgac gcagccggga
       61 cgacgaaata tgtcagcccg accggcggaa cctccatcac ccaggcagcc gatggtgtga
      121 tgtacaagaa cgcactgccc cggtatgtgg gcggttggac aaacagcttt gcctacaaag
      181 gctttagcct cgacgtcctg ctgacctacc aggccggctt ctccgtctac tacggtacca
      241 acgccggtct ccatgaccaa cggttctgga acaataccac cgacgtgctc accgacgcct
      301 ggtccgccaa aagcgccaac aacgccaaat atgccaaacc catcttcggg gataacgtct
      361 ccaacggctc ggccatgccg atggacatca acgtcttcaa gggcgacttc ataaaactca
      421 ggaatataac cctcagctat agtgtaccgc cctccatgat gcagcgcatc ggactcagca
      481 gcgccagggt ctacatcagc ggccagaacc tgggtataat gaccaaatat cccggtcctg
      541 atcccgaagt ttcctccaac gggaacagca acggcggcca gggcgttgac aggaacacgg
      601 tgcccaatgc ccgtaccttc accgtcggcc tgaatgtcgg cttctgatcc aaacccattt
      661 ccgaacacaa aagcacaaca acaatgaaaa aatataacca atatatagct cccttcctgc
      721 tcggcctcat ggccctgggc tcctgccaga aaaaagacct ctaccccgta ccccaaaccg
      781 ccgtttctga tgcttccgca ttcagtacgg ccggccggat ccagtcccag gtactgtccc
      841 tgtatggcgc actgaaagcc ggcggtttct acggcggccg ttatgtcatc gacggcgaca
      901 tcaaggcgga taacttcatc aacgaacagt ccaacctcgt caccggcgca gacgtctgga
      961 acgagaaccc taccaatagc gccacagcgg ttaccggcct ttggtcagcc gcctacctca
     1021 cgatcaacgc ctgcaacctc ttcatcgacg ggatgaacgc cggtggcaca aaagtggttg
     1081 gccccaccgc cgcaggcaac tacatctccg aagcaaaact catccgggcc ctttcctatt
     1141 attcgctgct gcaatactac gccctcccct atgccaataa caacggcgcc aacaaaggcc
     1201 ttcccctccg gctcaccggg atcaagagcg gcggcttctc gctcctggcc cgcagcccgg
     1261 tagacagcat ctacacgcag atcctcagcg acctcaacga cgctgaagcc ggcctgccgc
     1321 taagcaacgg ctccgcatac aacaacgtta cccgggcaca ccgcaacacg gccatcgccc
     1381 tcaagacaag ggtctacctg agcatgcaga actacaacgc cgtgatcacc gaagcaaata
     1441 agatcgtcgg cccctcggcc cccttcacct ccccttcggg cgtgaccttt gcgctgcaat
     1501 ccgatatcac caaggtgttc gtagcaccct acacgacatc ggagtcgatc ctctccctgc
     1561 ccatgtcctc caccacgggt gacaaccccg gtacgcaaaa ccagttgggc ttttacttct
     1621 ctccgtcgaa aggcgccgtt tccggcggca ccggcaacgg agaatactcc cttaaccccg
     1681 ccggtgtgat cgccgatccg aaatggacgg ccaccgatag aagaaggacc ttcatcgtca
     1741 cttctacaac aggcggaaag aaatggctca acaaataccc ggcgccaagc ccctacacgg
     1801 atttccccat cgtcatccgc tattcagaag tgttgctaag cctggccgaa gcccgtgtcc
     1861 ggtcaaccaa ttccatcgat gcccaggccg tggccctgct caatgccgtc cgcagccgtt
     1921 ccgatgcaac cactacctat accgtggcaa gtttcgccgg cacgagcgac ctgatcaacg
     1981 ccatcctgca agaacgcaat atcgaattcc tcggcgaagg cctgcgcaac aacgacctga
     2041 tgcgcctgca actgacggtc ccggcgaagg gcagcgcacc cgcaaaaagc cccagcgatg
     2101 taggctatat ctggccgatc tccgcaacag aacttagcct gaacaccttg tgcaccgata
     2161 actagttctc tacctgctac tacatatgag cccattcaaa aagggccgcc tcacactgag
     2221 gcggcccttt tattacttta taacccatcg gttattccac caccgatcgg gtggcagcga
     2281 tgatcttccc gctatcctgg tcctgtaaaa aagcgatcac ttccccatcg gcggcattca
     2341 cacccgccgg ccagtcaaga tgcccactgg cattcccctt cccatccgaa ggctgtgtct
     2401 ccaaaccacg gacgatctgt acatgcgaaa gcgtcctgcc cccattctcc cccgccttca
     2461 cactggtcac ggcagaatgc tccaccaccg ccaccaccag gctcgtcctg ctccccgcac
     2521 cctctatctt cgcctgccag gtcaaccgcc cgccactcag ccgaaccccg ctaagcgata
     2581 ccccggccac ggccccctcc tgcagactgc tgctgatcgt ccggcgaagc gaccccgcat
     2641 cggatccgac aaactccttc cgcccgttga ccaccacctg cggcgtatac accgacgaaa
     2701 gcctgagcca gcgcgcatac tcccgctgcc tgtccgaata ggccgcatcg ctgaacgcat
     2761 ccttccaccc aaggcggtcc caatagtcca catgaaagga aaggatatag accggcagat
     2821 ccttgtcctc cttctgtacc gtcgccacca gcgcatccgc cgccggacag ctgctgcaac
     2881 cctcggaagt gaacaactcc accaccgcaa accccttcgc cgcgggcgcc atcccaaaca
     2941 gcacatctcc cttccccgtc gcaaggccgt gccccttata tacaaatgca ccggctatca
     3001 tcgccacacc cgccaggccg gccgccagca gcagttttct acccgttttc ataatctgtt
     3061 ttttttatac cacaaatttc gtcaatattt ccccgccgca ccccggccat ttctcacaat
     3121 tttataaagc ttttactgcc ccggcaggcc gcggcacggc tttcgctgtc attatctcca
     3181 attaaaacac ctttttatgc ttgtctatac cagggcattc agcagctact ccgtcgacga
     3241 cctcgcaaag gcaaaagaat tctatgagaa gatccttggc ttcccggtaa cccaaacccc
     3301 cgaaggactc agcatgcagg tcacaaaaga ctattcggtc ttcctctacc caaaacccaa
     3361 tcatgagcca gccaccttta ccgtactgaa cctgcaggta atgagcgtgg atgccgccgt
     3421 cgacgcactc accgccgcgg gcgtaaaatt cgaacagtat gaaggggaaa taaaaacgga
     3481 cgaaaaaggc atctttcacg gcggaccggg aaaaggaccg accatcgcct ggttcgccga
     3541 ccccgcgggg aatatcctct ccgtcgtgga aaacccgccc gcctgagccg ccggcagccc
     3601 acatccaacc ccgggccctc taaaacacca gtaaagggtg ctgccccgcc gtgtgcagat
     3661 cccgccacac ccggttgatc tccgtatcca cccgcgcggc gccaagccct gcatagggat
     3721 acaacctgtc tacccattcc cggaccgcca gggccagcgc atggctgaca cgtcccactt
     3781 ccccgaacaa tgccggcgac gccgcccctt tctccaccgc ctcccagcta tggtccagcg
     3841 cccgaaagaa cgctcccctc gcctcggcca actccaaccg cgcttccgcc agcaacccct
     3901 ccatagcacg cgccgcaccg ggtttcatgc ggcc
//
LOCUS       DATGFG010000786         5214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_634410, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000786 DATGFG010000000
VERSION     DATGFG010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634410"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VKQ52_14745"
     CDS             complement(<1..644)
                     /locus_tag="VKQ52_14745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLZ88509.1"
                     /translation="MSVPSLLHTYRRYRTLQTDRITALPIVILMPHSACNCKCVMCDI
                     WKGNHQLKQLTEKDVEGLMTSLRRFGTQQVLMSGGEALLNPNFFRLCAILKKEDIRIT
                     LLSTGLALARHADNILEYVNDIIVSLDGDEALHDTIRNIPGAYRKMKQGIQLLHSRRP
                     DFRITGRTVIHRLNFRHWPAIIAAAQELGLQQISFLPADVTSEAFNRTNPWDEP"
     gene            complement(653..1420)
                     /locus_tag="VKQ52_14750"
     CDS             complement(653..1420)
                     /locus_tag="VKQ52_14750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ88510.1"
                     /translation="MYLPDSSILTNIQVQGHTRLREILFEKKYIQVRSLESRLYTDEE
                     LVHLPDITPAHPRFREWQLRKGSARRLVRYFSRKKTNLDILEVGCGNGWLTHQLAEIP
                     GSRVIGADINFTELQQAARVFSNDPNLRFIHGDIRSGLLEDRRFDYIIFAATLEHFPS
                     VKKILHLCLAHLKRGGEIHLLDTPFCKPEEVKLEERRTLAYYTSFGYPEMADYYFHHT
                     FDDLRSFRYTVLYNPKDFRHRFIKHRNQHPWIRITNN"
     gene            complement(1436..2869)
                     /locus_tag="VKQ52_14755"
     CDS             complement(1436..2869)
                     /locus_tag="VKQ52_14755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLZ88511.1"
                     /translation="MQIAASVEGKYEWVIVDGNRETDPYKKIRSYLATGGFKYVGFTV
                     MPGPQLKQAIPFSRRIREEFPGIVMIWGGYFASNQHKVVLKSGYVDFVINGPGDHAFP
                     ALVDALETGKPYEGIKNLIYRTGDRIVKTAKEDLVDQSTLPRLPYDQLDKFYSLEKYL
                     GKTYLGDRTLAYHSSMGCPFTCSFCAVVPIYEARWKAKPADQVYTDVRYLRDKWGADS
                     IEFHDNNFFVSEKRTAEFSRLVKNDRMIWWGEGRIDTIDKYKDETLALMRESGCKMIF
                     FGAETGNDEILKSMDKGGTQSGAQIRAFAARMKKFDIIPEYSFVLGTPADTPEKVMQY
                     IDQDIAFIKEIKSINPDTEIVIYTYSPVPTEGSELFKKSQASGFSFPEKLEEWISPHW
                     ENFDLRKNPLTPWLTPEMIDKIRDFETVLNGYYPTVSDIRLSTIKRKVIHTVAALRYK
                     KNLYKYPYEIKLLHKIWRYRQPEIQGF"
     gene            3008..3754
                     /locus_tag="VKQ52_14760"
     CDS             3008..3754
                     /locus_tag="VKQ52_14760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ88512.1"
                     /translation="MPILRKIKETLLPSRRPVTEPAAAYDIWALSYDHQPDNLMLALD
                     ERICTDLFGRTVIDGRVVADIGCGTGRHWNKLLSHRPARLVGYDVSSGMLDILRKKYP
                     QAETYLLQGGLLSELGTGSVDLVVSTLTIAHIPNIVAALVEWNRILKPGGEMILTDYH
                     PAALVKGGQRTFTDSGKVIAIRNHVYPIRKIRAIARKLGFRELTVIEKRIDETMIPYY
                     EKQNALATYQRFQGVPIIYGIHLKKSDVAE"
     gene            3741..4892
                     /locus_tag="VKQ52_14765"
     CDS             3741..4892
                     /locus_tag="VKQ52_14765"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ88513.1"
                     /translation="MWLNNLRLPGQETAIDLLVEGNAIHGVRPANGSLPGLSFDGALV
                     FPGLINSHDHLDFNLFPALANRIYTNYRDWGKDIHTANKAAIGAVLRIPQSLRTQWGV
                     YKNLLNGFTTVVNHGERLAIPADLITVFQECHCLHSVGFEKNWRWKLNRPLRDHHPFV
                     LHAGEGTDAPAGEEIGRLIRWNLFNRPLIGVHGVAMDERQAAFFRALVWCPSSNYFLL
                     DRTAPVSRLKEVVPILFGTDSTLTADWNSWRQIRQARQERAATDGELLAMLTTAPAAA
                     WRLGDRGELAKGKRADLVIARGKPGLSGMDAFFGLDPEDMLLVLHEGQIRLFDVSLRE
                     MLMAAGMKDMVFDFSRCGNKYVAGDLPGLMEEIRGYYPEAEFPGMLITG"
     gene            complement(4882..>5214)
                     /locus_tag="VKQ52_14770"
     CDS             complement(4882..>5214)
                     /locus_tag="VKQ52_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88514.1"
                     /translation="RRIVVAEAPAKYKPMIYQLGRNHSAAPPPLNELPFGTVQTREFG
                     FFVPEWVESYKEITIDKTLDLGSHMLLCGQWHDDVQLKPATPRLHHIHFLHFLYLKGR
                     GLTYPVIQ"
BASE COUNT         1066 a         1272 c         1624 g         1252 t
ORIGIN      
        1 cttggttcat cccagggatt tgtccggtta aaggcttcgc tggtgacgtc ggcggggagg
       61 aagctgatct gttgcagccc cagttcctgt gcggcggcga tgatggcggg ccagtgccgg
      121 aaattcaggc gatggatgac cgtgcggccg gtgatgcgaa aatccgggcg ccgggaatgg
      181 agcagctgga tgccctgctt catcttgcgg taagccccgg ggatattgcg gatggtatcg
      241 tgcagggctt cgtcgccgtc gagggagacg atgatgtcgt tcacgtattc gaggatgtta
      301 tcggcatgtc ttgccagggc caggccggtg gacaggaggg tgatgcggat atcttccttt
      361 ttgaggatgg cgcatagccg gaagaaattg gggttgagca gggcctcgcc acccgacatg
      421 agcacctgtt gagtgccgaa acggcggaga ctggtcatca gtccttcgac atctttttcc
      481 gtgagttgtt tcagctgatg attccctttc cagatgtcgc acattacgca tttgcaatta
      541 caggcgctat ggggcatgag gataacgatc ggcagggcgg tgatcctgtc ggtctgtagt
      601 gtacggtaac gccggtatgt gtgcagcagc gatggaacgc tcatattttg ttctagttgt
      661 tcgtgatgcg gatccagggg tgctgattcc tgtgtttgat gaagcggtgt cgaaaatctt
      721 tcgggttgta caaaacggta taccggaagg accgaagatc atcaaaggta tggtgaaaat
      781 agtagtcggc catttcgggg tagccaaaag aagtataata ggccagggtc cttctttctt
      841 ccaactttac ctcttcgggt ttgcagaagg gggtgtccag caggtggatc tcgccgccgc
      901 gcttcagatg ggcgaggcag aggtggagga tctttttcac cgaggggaag tgttcgaggg
      961 tggcggcaaa gatgatatag tcgaagcgcc ggtcttcgag caggcccgag cggatatcgc
     1021 cgtggatgaa ccggagattg gggtcgttgc tgaagacccg ggcggcctgt tgcagttcgg
     1081 tgaaattgat gtcagcgccg atgaccctgg agccggggat ctcggctaat tggtgggtaa
     1141 gccagccgtt gccgcaaccg acctcgagga tatcgagatt ggtctttttg cgggagaagt
     1201 accggacgag acggcgggcc gacccttttc tgagctgcca ttccctgaag cggggatggg
     1261 caggggttat atcaggcaga tgaaccagtt cttcatcggt gtacaggcgg ctttccagcg
     1321 agcggacctg gatgtatttc ttttcgaaga ggatctctct gaggcgggtg tggccctgaa
     1381 cctgaatatt ggttaatatg gaagagtcgg ggaggtacat aaaaagtgat tttggttaaa
     1441 atccctggat ctccggctgt cgatagcgcc agatcttgtg caggagtttt atttcgtagg
     1501 ggtatttgta cagatttttt ttataacgta aggcggcgac cgtatggatg accttccgtt
     1561 ttatcgtgct gagccggata tcggatacgg tgggataata gccattgagg acggtctcga
     1621 aatcgcggat cttgtcgatc atttccgggg tgagccaggg tgtcaggggg ttcttgcgaa
     1681 ggtcgaagtt ctcccaatgg gggctgatcc attcctcgag tttttcgggg aagctgaacc
     1741 cggaagcctg gctttttttg aatagttcgg agccttccgt cggtacgggg ctataggtat
     1801 agatgacgat ctcggtatcg gggttgatgg attttatctc ttttatgaaa gcgatatcct
     1861 gatcgatgta ttgcatcacc ttttccgggg tatcggcggg tgtgccgagc acgaaggaat
     1921 attcgggaat gatatcgaat tttttcatcc gggcggcgaa ggccctgatc tgggcgcccg
     1981 actgggtacc acccttgtcc atactcttca ggatctcgtc attacctgtt tcggcgccga
     2041 agaagatcat tttacaaccg gattcgcgca tgagcgccag ggtctcgtcc ttgtacttat
     2101 cgatcgtgtc gatgcgccct tccccccacc agatcatcct gtcatttttg acgaggcggg
     2161 agaattccgc ggtgcgtttt tccgatacga agaagttgtt gtcgtggaac tcgatggaat
     2221 cggcccccca tttatccctc aggtagcgga catcggtgta cacctggtcg gccggttttg
     2281 ccttccagcg tgcttcgtag atgggtacga cggcgcaaaa gctacaggtg aagggacagc
     2341 ccatgctgga gtgataggcg agggtacgat caccgagata ggtcttgccg agatatttct
     2401 ccaacgaata gaatttgtcc agctgatcat agggcagacg ggggagggtg gattgatcga
     2461 cgaggtcttc ttttgccgtt ttgacgatgc gatcgcccgt ccggtagatc aggtttttta
     2521 tcccttcata gggtttgccg gtctcgagtg cgtcgaccag ggcgggaaag gcgtgatcgc
     2581 cgggaccatt tatcacgaag tcgacgtatc cgcttttcag gaccaccttg tgctggttgg
     2641 aagcgaaata gccgccccag atcatgacga tgccgggaaa ctcctcgcgt atgcggcggg
     2701 agaagggaat ggcctgcttc aactgcgggc cgggcattac agtaaagccc acatatttaa
     2761 agccgccggt ggcgagatag gaccggatct ttttataggg gtcggtctcg cggttgccgt
     2821 caacgatcac ccattcgtac ttaccttcca ccgatgctgc gatctgcaag atggaatttg
     2881 gaatcctgaa tttataagtc gcacttcttg gattgaataa caatatctta cacatgacta
     2941 ccctttaact tagatttttc ctttttgagg catcccggaa gaataaacgt ccgttttata
     3001 ggaaaggatg cctatactac gcaaaatcaa agagaccctc ctgccctccc gccggcccgt
     3061 caccgaaccg gcggccgcct acgacatctg ggcattgagc tatgatcatc aaccggataa
     3121 ccttatgctg gccctggacg aaaggatctg tacggatctc ttcggccgaa ccgtcatcga
     3181 cggccgggtt gtagccgata tcggctgtgg cacgggccgg cattggaaca aattactgag
     3241 tcaccggcct gcccgcctgg tgggttatga cgtatcctcc ggcatgctgg atatactcag
     3301 gaagaaatac ccgcaggccg agacctatct gctccagggc gggcttttat cggagttggg
     3361 gacggggagt gtcgatcttg tggtctccac cctgacgatc gcgcatatcc cgaatatcgt
     3421 cgccgcgctg gtcgagtgga accggatcct gaagcccggc ggtgagatga tcctcaccga
     3481 ctatcatccc gcggcgctgg tcaagggagg tcagcggacc tttactgaca gcggcaaggt
     3541 catcgccatc cgcaatcatg tctacccgat caggaagatc agggcgatcg cgagaaaact
     3601 gggtttccgg gaattgaccg tcatcgagaa aaggatcgat gagacgatga taccgtacta
     3661 tgagaaacaa aatgcattgg ccacctatca acgtttccag ggtgttccga tcatttatgg
     3721 tattcaccta aagaaatccg atgtggctga ataacctgcg gcttcccggc caagaaacag
     3781 cgatcgacct cctggtggaa gggaatgcca tccatggagt gcgtccggcc aatggcagcc
     3841 ttccggggct ttcctttgac ggcgcgcttg tcttccccgg gcttatcaac tcccacgatc
     3901 atcttgactt caacctgttc cccgctctgg ccaaccgcat ctataccaat taccgggact
     3961 gggggaagga tatccatacg gccaacaagg cggccatcgg ggccgtgttg cggatcccac
     4021 agtcattacg gacgcagtgg ggcgtctata agaacctgct caatggtttt accaccgtgg
     4081 tcaatcatgg cgaacggctt gccattcccg ccgacctgat cacggtcttc caggaatgtc
     4141 attgtctgca ttccgtcggg tttgaaaaga actggcggtg gaagttgaac cggccgctga
     4201 gggatcatca tccctttgtg ctgcatgccg gggaagggac ggatgctcct gcgggggagg
     4261 agatcgggcg gcttatccgg tggaaccttt ttaaccggcc gcttatcggg gtgcatgggg
     4321 tagcgatgga tgagcgtcag gcggcttttt tccgggccct tgtctggtgt ccgtcctcga
     4381 attattttct gctggacagg acggcgccgg ttagccggct aaaggaggtg gtccccatcc
     4441 tgttcggcac cgatagcaca ctgacggccg actggaacag ctggcggcag atcaggcagg
     4501 cccggcagga gcgggccgcg acggacgggg aattgctggc gatgctgacg accgcgcccg
     4561 cggcggcctg gcggcttggc gaccgggggg aattggcgaa ggggaagcgg gcagaccttg
     4621 tcattgcccg gggtaaaccg ggcttatcgg ggatggatgc tttttttggg cttgatcccg
     4681 aggacatgtt actggtgctg catgaagggc agatccggct attcgatgtc agtctgcggg
     4741 aaatgttgat ggcggccggg atgaaggata tggtgttcga cttttcgcgt tgcgggaata
     4801 agtatgttgc cggcgatctg cccgggctga tggaggagat ccgcggctat tacccggaag
     4861 cggagttccc ggggatgctg atcactggat gaccggatag gtcaggcccc tacccttcag
     4921 gtacaggaaa tgcaggaaat ggatatggtg caaccggggt gtggccggct ttagttgtac
     4981 gtcgtcatgc cattgaccgc agagcagcat gtggctgccc agatcgaggg tcttgtcgat
     5041 ggtgatctct ttatagcttt ccacccattc cgggacgaag aaaccgaatt cccgggtctg
     5101 gaccgtcccg aaggggagtt cgttgagggg cggcggcgcg gcgctatgat tcctgcccaa
     5161 ctgatagatc atgggcttat attttgcggg ggcttcggcg accacgatcc tgcg
//
LOCUS       DATGFG010000787         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1357895, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000787 DATGFG010000000
VERSION     DATGFG010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357895"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2028)
                     /locus_tag="VKQ52_14775"
     CDS             complement(<1..2028)
                     /locus_tag="VKQ52_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HLZ88515.1"
                     /translation="MHCRLLIFLCLTAFTAFAQPRRDILLNTGWRTSLIDSNNWRSVD
                     IPHNWDDYAGYRRLRHGNLHGSALYTRTFSVTRQPGKRYFLWFEGVGSYATVRVNKQP
                     VGTHAGGRTSFTLDITDALKTDALKTGAATNDLEVRADHPANIRDLPWVCGGCSDERG
                     FSEGSQPLGIFRPVHLIITNETRIEPFGVHIWNDTSISERSATLYLETELKNYGTTPL
                     AAEITQQLLDKTGTPVATVTQTIHIDGTTTATKSIHLTGTTTAAQPVPIHIPGTTTIR
                     QELPNIRTPHLWSPSSPYLYTLVTTIRNRGGIIDQTTTAYGIRRIHWTDPAATGPHPF
                     TVNGRPVFLNGIAEYEHRIGNSHAFTHAEIKARAQQIRAAGFNAFRDAHQPHNLYYQH
                     FWDSLGILWWPQFSAHIWFDSPGFRENFKTTLTEWIKERRNSPSVILWGLQNESKLPE
                     DFAKECVALIRQLDPTASGQRLIVTCNGGSGTDWDVPQNWTGTYGGDPATYGKDLIRQ
                     VLVGEYGAWRTADLHGDTTNSEDRMCALMEQKIRLADSVRSQVAGHFAWLYTSHDNPG
                     RVQSGEGERELDRIGPVNYKGLLTEWEEPLDPYYLYRANFTDPHTDPMVYIVSHSWPH
                     RWETPGIKSGIIVYSNCDTVELFNGKTPLGIRTRHDIGTHFQWDSVDIQYNT"
     gene            complement(2028..>2876)
                     /locus_tag="VKQ52_14780"
     CDS             complement(2028..>2876)
                     /locus_tag="VKQ52_14780"
                     /inference="COORDINATES: protein motif:HMM:NF045341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88516.1"
                     /translation="DHQIIRDLFRNCIAAESILRPGTAERGSTEGQTLAVDANRPIFR
                     HRAIRAQHPEFQNELQSKLERIAPNTIGRFGQLQEWLRDKDDTADKHRHISHLWGVYP
                     GTDITWHDSAMMKAARQSLIYRGDDGTGWSLAWKVNCWARFRDGDHALKLLDKLLSDA
                     VGATGEHGGVYPNMFDAHPPFQIDGNFGGAAGVAEMLLQSQDNGIDLLPALPSALPDG
                     EVRGLRARGGFTVDLTWRDGNLQSLALTSAAGGPCTIRYRDQTRTLATQPQHTYHFDA
                     QLKSLP"
BASE COUNT          478 a          663 c         1097 g          638 t
ORIGIN      
        1 ggtattgtat tggatgtcga cgctatccca ttggaagtgg gtgccgatgt cgtggcgggt
       61 gcgtatgccg aggggggtct tgccgttgaa gagttcgacg gtatcgcagt tggagtagac
      121 gatgatgccg gactttatgc cgggagtctc ccagcgatgg ggccagctgt gggagacgat
      181 gtagaccatc gggtcagtgt ggggatcggt gaagttggcg cgatagagat agtaggggtc
      241 gaggggttct tcccattcgg tgagcaggcc cttgtagttg acggggccga tgcggtcgag
      301 ctcgcgttcg ccttcgccgg actggacgcg accgggattg tcgtgggaag tgtagagcca
      361 cgcgaaatga ccggcgacct gggatcttac actgtcggcg agccggatct tctgttccat
      421 gagggcgcac atccgatcct cgctgttggt ggtgtcgcca tggagatcgg cggtgcgcca
      481 ggcgccgtat tcgccgacga ggacctggcg gatgaggtct tttccgtagg tggccgggtc
      541 gccaccgtag gtaccggtcc agttttgtgg aacgtcccag tcggtgccgc tgccgccgtt
      601 gcaggtgacg atgagccgct gacctgacgc ggtcgggtcg agctgccgga tgagggcgac
      661 gcattccttt gcgaagtctt cggggagttt gctttcattt tgcaggcccc agagaatgac
      721 cgaagggcta ttgcgccgct cctttatcca ttcggtgagg gtggttttga aattctctct
      781 gaacccgggg ctatcgaacc agatgtgcgc ggagaactgg ggccaccaga ggatgccgag
      841 gctgtcccag aaatgctgat aatataaatt atgcggttga tgggcgtcgc ggaaggcgtt
      901 gaagccggcc gccctgatct gttgcgcccg ggcctttatc tccgcgtggg taaacgcatg
      961 gctgttgccg atgcgatgtt cgtattcggc gatgccgttg aggaagacgg gccggccgtt
     1021 gacggtgaag ggatgcggac cggtggcagc ggggtccgtc cagtggatgc ggcggatgcc
     1081 gtaagccgtt gttgtctgat cgatgatgcc gccgcggttc ctgatcgtgg tgacgagggt
     1141 atagagatac ggcgacgatg gggaccagag atggggtgtt cttatgttgg ggagttcctg
     1201 gcggatggtg gtggtcccag ggatgtggat ggggacgggt tgagcggcgg tagtggtgcc
     1261 ggtgaggtgg atggatttgg tggcagtggt cgtcccatcg atgtggatgg tttgggttac
     1321 ggtggcgacg ggggtaccgg ttttgtcgag gagctgttgg gtgatctctg cggcgagggg
     1381 tgtggttcca tagttcttta gctcggtttc gaggtatagg gtggctgatc gttcgctgat
     1441 ggaagtgtcg ttccagatgt ggacgccgaa gggctcgatg cgggtttcgt tggtgatgat
     1501 gagatggacg gggcgaaaga tgccgagggg ctgggatcct tcggagaagc cgcgttcgtc
     1561 ggagcagccg ccgcagaccc aggggagatc gcggatattg gcggggtgat cggcgcggac
     1621 ttcgaggtcg ttggtggcgg cgccagtctt aagagcatca gtcttaagag cgtctgtgat
     1681 atcgagggta aaggaggtgc ggccgccggc gtgtgttccg acgggttgtt tgttgacccg
     1741 gacggtggcg taggagccga ctccttcgaa ccagaggaag tagcgtttgc cgggttgcct
     1801 ggtgacggag aaggttcttg tatagagggc gctgccgtgg aggttgccat ggcggagacg
     1861 gcggtagccc gcatagtcgt cccagttatg ggggatgtcg acggagcgcc agttgttgct
     1921 gtcgatgagg ctggtgcgcc agccggtgtt cagcaggata tcgcgccggg gttgggcgaa
     1981 ggcggtgaag gcggtgaggc agaggaatat gagcaggcgg caatgcatca gggcagggat
     2041 ttcaattgag cgtcgaaatg gtaggtatgt tgcggttggg tggcgagcgt gcgggtttgg
     2101 tcacggtagc ggatggtaca gggaccgccg gcggcggagg tgagcgcgag ggattggagg
     2161 ttgccgtccc gccaggtcag gtcgacggtg aagccgccgc gggcgcgcag gcctcttacc
     2221 tcgccgtcgg ggagggcgct ggggagggcc gggaggagat cgatgccatt gtcctggctt
     2281 tggagcagca tctcggcgac gccggctgcg ccgccaaagt tgccatcgat ctggaacggc
     2341 ggatgcgcgt cgaacatatt cggatatacg ccgccgtgtt cacccgtggc gccgacggca
     2401 tcggagagca gtttatcgag caatttcagg gcgtggtcgc cgtcgcggaa gcgggcccag
     2461 caattgacct tccaggcgag gctccagccg gtgccatcat cgccgcgata gatcagcgac
     2521 tggcgggcgg ccttcatcat cgcgctgtcg tgccaggtga tatcggtgcc ggggtagacg
     2581 ccccagagat gcgagatatg gcggtgtttg tcggcggtgt cgtccttgtc ccggagccat
     2641 tcctgtagtt ggccgaaacg gccgatcgta ttgggggcga tgcgttccaa ttttgattgg
     2701 agctcgtttt gaaattcagg gtgttgtgcg cgaatagccc tgtggcgaaa aataggtcta
     2761 ttcgcgtcaa cggctaatgt ctgaccttcg gtcgaacctc tttcagcggt tcctggccgg
     2821 aggatgctct cggcggcaat gcagttacgg aacagatcgc ggatgatctg gtgatc
//
LOCUS       DATGFG010000788         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1310603, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000788 DATGFG010000000
VERSION     DATGFG010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310603"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VKQ52_14785"
     CDS             <1..628
                     /locus_tag="VKQ52_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ88517.1"
                     /translation="HIKRIGIFFPADMLRQHLRKDILDDLFRYTDTHPTPSAKEPAPF
                     EYRSVIEDVFSIDNASALSHLILHNRVLLLAEKFLNAFLTKAPFSLPDGKAWIKGKEK
                     DLDALKSIVQILSDNQLNKFPSIESLSKTAMMSSTKLKSRFKQIYGMKLYEFYNRNRL
                     EQAHEMLKTGDFSVKQVGINIGFSNLSNFAKAFKKEFGILPKEVLKNK"
     gene            868..1848
                     /locus_tag="VKQ52_14790"
     CDS             868..1848
                     /locus_tag="VKQ52_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ88518.1"
                     /translation="MVMYSFHLENFEKWLAGLADRLNTTVTDRSLSIPAHLGSGTIQA
                     QNINPHLSYAVMNFRLDGDLEFHRESGATGFVISFNLVEVQKELLVGHRQNIISDKRP
                     FRNDIFLTDARDSTFIRISAGSTVRRLLIFCSHELAMQYLPQEIFTKLAVFARDNSIS
                     DHPLFINLSHRTMLKELFELKENDALMPIRRLSGIIHLIEKFLHSFLRQEQAALPRTV
                     KKNDLESMQHVEHILSSKLEGFPSLESLAQEVFMSTSKLKNLFKQVYGHTLYDYYNKS
                     RLQRAKDLLITGQCSIKQAGSEIGFSNLSHFAKAFRKEFGILPRDLVKGR"
     gene            complement(2097..>2797)
                     /locus_tag="VKQ52_14795"
     CDS             complement(2097..>2797)
                     /locus_tag="VKQ52_14795"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLZ88519.1"
                     /translation="SGAVRMTTAEPETDRQERIFSYILADGYYVCGGATNNGGNVLQW
                     YAGQVLGRKGADAGELERLVAEADEVPPGAEGLVFLPYLLGERAPIWDARARGVFFGV
                     RSIHDQRHFLRAVLEGVSFSLRQIGASLEETIGPIGQIHASGGFTQSATWLQLIADVF
                     QKNVAVSGSADASAIGAAQMGLYALGIVPSLDAAASLVRTEAVYEPDAGRAAVYRENY
                     AVFTELYRRLRDLM"
BASE COUNT          722 a          885 c          598 g          592 t
ORIGIN      
        1 ccacatcaaa cgcatcggca tcttcttccc ggccgacatg ctccggcaac atctccgtaa
       61 ggatatcctc gacgatcttt tccgctacac cgacacccat cccactccct ccgccaaaga
      121 gcccgccccc ttcgaatacc gttcggtgat cgaagatgtc ttcagcatcg acaacgcctc
      181 cgcactttcc cacctcatcc tccacaacag ggtcctgtta ctggccgaga aattcctgaa
      241 cgccttcctc acaaaagccc ccttctccct gcccgatggt aaagcctgga taaaaggcaa
      301 ggaaaaagac ctcgacgcat taaaaagtat cgtccagatc ctcagcgaca accagctaaa
      361 caaattcccc tccatcgaaa gcctctccaa gacagccatg atgagctcca ccaaactcaa
      421 atcacgcttc aagcagatct acggcatgaa gctgtatgag ttctacaatc gcaaccggct
      481 cgaacaggcg catgagatgt tgaagaccgg cgacttctcc gtcaaacagg tgggcatcaa
      541 catcggcttt tccaacctca gcaattttgc aaaggccttc aagaaagaat tcggcattct
      601 gcccaaagaa gttcttaaga ataagtaact atcttaccga tcactaagta acatcttata
      661 aatactcttg aaagttttgt taaatagcct tttcagctat taaaagagag tttatagcct
      721 cacgccatcc cgaattaacc gtaattttgc gcctttgttc cgctaagata atcccactca
      781 gccctaccgc gactaaattg ccattgagag aaaagtattg gacttcaagt tccaagaaaa
      841 ttgtaaacgt gtcaaaaaca ggtgaatatg gtaatgtact ctttccattt ggagaatttt
      901 gaaaaatggc tggccggtct tgccgaccgt ctcaacacga cggtaaccga ccgttccctc
      961 tccattcccg cacatctggg ctcgggaact attcaggccc agaacattaa tccgcatctt
     1021 tcttacgctg tcatgaactt caggttggac ggcgatctgg aatttcaccg cgaatccggc
     1081 gccaccggct ttgtgatctc ttttaacctc gtcgaagtac agaaagaact gctggtaggt
     1141 catcgccaga acatcatcag cgataaacgc cccttccgca acgacatctt cctcaccgac
     1201 gcccgcgaca gcaccttcat acgcatctcc gcgggcagca cggtcagaag attgctcatc
     1261 ttctgttcgc acgaactcgc tatgcaatac cttccacagg agatctttac caaactggcg
     1321 gtattcgcac gcgataacag tatcagcgac caccccctct tcatcaatct gtcccaccgg
     1381 accatgctca aggagctttt cgaactcaag gagaatgatg cgctgatgcc catccggcgc
     1441 ctgtcgggta tcatccacct gatcgaaaaa ttcctgcatt ccttccttcg ccaggaacag
     1501 gcagcactgc cgcgtaccgt gaagaagaac gatctggaaa gtatgcaaca cgtagagcat
     1561 atcctcagca gcaagctcga aggcttccct tcgctggaat ccctggcaca ggaagttttc
     1621 atgagcacca gcaaactgaa gaacctcttc aaacaggtgt acggacatac gctgtatgac
     1681 tactacaaca agagccgctt acaaagagca aaagacctcc tcatcacagg ccagtgcagt
     1741 atcaaacagg ccggcagcga aatagggttc agcaacctga gccacttcgc caaggccttc
     1801 cggaaggagt ttggtatcct gcccagagac ctggtaaagg gccgctagga cagccatcgg
     1861 gacacccgat acagctatcc attgccctta aagagtcata ttcctgacgt gtagaggtac
     1921 tatatcgatt ttacaattgt tagatttaca attgtaatca agcccgtttt tggtccaatt
     1981 atccttgcca gattattcat cattcaggcg cgcgtagcaa ccggcgatcc gtctaacagg
     2041 ggggtcgccg tttttcttcg tccatgaccc gccgcctgca gacatgcgcc tgccgcttac
     2101 atcaggtccc ttagcctcct gtacaactcc gtgaaaacag catagttctc ccggtacacc
     2161 gccgccctgc ccgcatcagg ctcatagacg gcctccgtcc tcaccagcga agcggcggca
     2221 tcgagactgg gaacgatccc cagggcatac aaacccatct gcgcggcccc gatagcggag
     2281 gcatccgccg aacccgaaac agccacattt ttctgaaaga catcggcgat cagttgcagc
     2341 caggtggcgc tctgcgtaaa cccaccgctg gcatggatct gcccgatagg cccaatagtc
     2401 tcctccaggg aagccccgat ctggcgcagc gaaaaactga caccctccag caccgcccgt
     2461 agaaaatgcc gctggtcatg gatcgaccgg accccaaaga acacccccct ggccctggca
     2521 tcccagatag gcgcccgctc accaagcaga taaggaagaa agacaaggcc ctccgccccg
     2581 ggcgggacct catccgcctc cgccacaagc cgctccagtt cacccgcatc ggcccccttc
     2641 cgccccaaca cctggccggc ataccactga aggacattcc cgccgttatt ggtggctcca
     2701 ccacagacat aatatccgtc tgcaagaata tagctgaaga tccgctcctg ccggtctgtt
     2761 tccggctcgg ccgtcgtcat ccggaccgcc ccgctgg
//
LOCUS       DATGFG010000789         5329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_17355, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000789 DATGFG010000000
VERSION     DATGFG010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17355"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VKQ52_14800"
     CDS             complement(<1..239)
                     /locus_tag="VKQ52_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88520.1"
                     /translation="MKFNAITRGLSCVGLGFLLITGLFSCKKDLRSSSPPAGVTTTPR
                     ATSYALVWDDEFNGSSVNTANWNIDVGNPGVNNEL"
     gene            459..1919
                     /locus_tag="VKQ52_14805"
     CDS             459..1919
                     /locus_tag="VKQ52_14805"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HLZ88521.1"
                     /translation="MIRLKIHLSLCSAALLLAAGAFAQSNSLYSYQDLSHFYYSKQRD
                     SLKKAWVCPNSFKEKAAQKKYRDIWDARTDAIIGAINDDDYVHDNEVYNYIDGIVREI
                     TEANRQMVPVKPFVLIDRSSSVNAYAVGGNVLAVNLGLITFATTREELALVIAHEMSH
                     NILHHVENAMYQKAEWLSSDEYRQSLNAVMDSKYERLSRLKKVLETYTFSRSRHQRYH
                     ESEADSLAVILLKKTGIPFQASYFLRLDSADLEYLQPLKHPVKAFFTAYHLPYDDSWT
                     QKRTHGLSSRAYSFSDTSKLADSLKTHPDCVDRYEKTRSLTTANPHFTAIPANVQEKA
                     NKMLLWNLYSGINLTPCLYRILQIKDKGNTDLWYDFMLSNCFAGLYYSDRMLNRFNAI
                     GVVPKEIISKDYYALQTLLEQIPRDNLKQACQTLQDGGFWKNMPTAEKDLKSFVFTLA
                     LDPDDSDKNKARAARLFSTGNANSMYNELAQNFEKK"
     gene            1921..3489
                     /locus_tag="VKQ52_14810"
     CDS             1921..3489
                     /locus_tag="VKQ52_14810"
                     /inference="COORDINATES: protein motif:HMM:NF042600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6770 family protein"
                     /protein_id="HLZ88522.1"
                     /translation="MKTLLRILCLLVLTTTAAQAQTKVFKAVADDMSQDFEPIIQDGN
                     LVGYLVFTQLERASADSFNYSIEIMDENLNDIGTVKFREEKLNLKAVSFDQDVLCLAY
                     VKTNFVGREFKNEKEFRNFKTEGKAALFTQFLGLNGKILASNSTKMQITPDFAYAANS
                     NRRVIGNGKLKHGILLRNISGTGFACFYGDDSKNNLIVFNTTGKLVWQKQIKETGDDF
                     TMLTSGPEVSLLVKMSDQMKEGGYQVLSYNAVDSTAYPKFLLKDKKGNSLKVLAFEND
                     PVSGKPYLAGLVIDPIRGNHYGYGRAISHGPYCGLFTINLNGHTKKDIQASFSYWNDG
                     SQNFIDKYGYLPQAHEYANIERAFRDYQGNTIFAACGITSKVRWGAITGAVLTVWTIY
                     GAPAFLAGGTSKYATRDILLMKQDAAGKLDLATTVPAPRSSYSFATFPVSSYDPNNYY
                     TVKNSDTRTQYLVIDKAKDIDIYNVNQKKVARTIPHKEGNNLVTVFPAKEGYVMVYEF
                     NKKEKTTRLSIEAL"
     gene            complement(3629..3859)
                     /locus_tag="VKQ52_14815"
     CDS             complement(3629..3859)
                     /locus_tag="VKQ52_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88523.1"
                     /translation="MILGQRWMGDLFKANAWWVLHGDDRVAADAAEGAPAIYGDPQFR
                     DPGNTQVKEVRGLAGYGKYKPGPALQGFGANF"
     gene            complement(3882..5042)
                     /locus_tag="VKQ52_14820"
     CDS             complement(3882..5042)
                     /locus_tag="VKQ52_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLZ88524.1"
                     /translation="MRRFISGPAALLMVFFLLGRLTVAGRNYYVDNSGDDGGDGGRAH
                     PWKTMDRVNRLRLQPGDSVFFRGGQSFTGVLRVEGEGGSAAHPAWIGVYGQGAATLPA
                     GDSSAVVFYRADWVRLKGLRLVGSGRKTGNVRDGLRLIECGQVRVDAIDVTGFQKSGV
                     LIYASKGVVISRVFAHENGSAGITVEGSEGKLSCRNITIVDCRADDNPGDPTNLTNHS
                     GNGIVAGHCTNLLIDRCAATNNGWDMPRIGNGPVGIWCYESDSVVIQHCLSYRNKTSV
                     GGADGGGFDLDGGVTHSIVQYCLSYGNQGAGYCLFQYWGASPWYHNIFRYNISEDDGA
                     VSDSHAGLYIWNSSGDSLQFHDCVVYNNTIYNTKVAAVSFSEKSAPGVCTSP"
BASE COUNT         1351 a         1765 c         1130 g         1083 t
ORIGIN      
        1 tccagttcat tgttgacacc cggattgccc acatcgatgt tccagttggc ggtattcacg
       61 ctactgccgt tgaactcatc gtcccacaca agggcgtagg aggtcgcccg cggtgtcgta
      121 gtcacacccg ccggggggga agaagaacga agatcctttt tgcaggaaaa caggcctgtg
      181 atcagaagaa agcccaggcc cacacaggag agcccccgtg taatcgcatt gaatttcata
      241 cgcaaacatt ttagtgataa gattcgagaa tcaaaatagc cctaaaatga cacgctgatc
      301 ctgaaaatat actcggaaaa cccctattcg gggaaaactt tcgcccaaag ccgccttccc
      361 cgcgccattc ccgcagcgga aagccgatac attcccccgg cggacagcca atatttttac
      421 cctttcgtcg ttatccatct aacctatctg taagcccgat gattcgcctt aagatccact
      481 tatccctttg ctccgccgcc cttctgctgg ccgccggcgc cttcgcccag tccaactcgc
      541 tttattccta tcaggacctt tcccacttct actacagtaa acaacgggat tccctgaaaa
      601 aggcctgggt ctgccccaac tccttcaaag aaaaagccgc ccagaaaaaa tacagggaca
      661 tctgggatgc acgcaccgac gctatcatcg gcgccatcaa cgacgatgac tatgttcatg
      721 ataacgaggt ctataactat atcgatggca tcgtccgcga gataaccgag gccaaccgtc
      781 aaatggtccc ggtaaaaccc tttgtcctca tcgaccgtag cagctccgtc aatgcctacg
      841 ccgtcggcgg caatgtcctc gccgtcaacc tgggcctcat cacctttgcc accacccgtg
      901 aagaactggc cctcgtcatc gctcacgaga tgtcgcataa tatcctccac cacgtcgaga
      961 acgccatgta ccaaaaagcc gaatggctca gctccgacga ataccgtcag tccctcaacg
     1021 ccgtcatgga ttccaaatac gaacgactgt cccggctgaa aaaagtcctc gagacctata
     1081 ccttcagccg cagccgccac cagcgctacc atgagagcga agccgattcc ctggccgtca
     1141 tcctcctcaa aaaaaccggc attcccttcc aggccagtta cttcctgcgc ctggacagtg
     1201 ccgacctgga atacctgcaa ccgctaaagc atccggtcaa agcgttcttc acagcctatc
     1261 atctgcccta cgacgatagc tggacacaaa aacgcaccca tggcctctcc tcccgggcct
     1321 attccttctc ggacaccagc aaactggccg actccctgaa gacccacccc gactgcgtgg
     1381 atcgctatga aaagacgcgg agcctcacta ccgctaaccc gcatttcacg gctatcccgg
     1441 ccaatgtcca ggagaaagca aataagatgc tcctctggaa tctatactcc gggatcaatc
     1501 tcaccccctg cctctatcgc atcctccaga taaaagataa aggcaatacc gatctctggt
     1561 acgacttcat gctcagcaac tgcttcgccg gactctacta ctccgatcgt atgctcaacc
     1621 gcttcaacgc catcggcgtc gtaccaaaag agatcatctc caaagactat tatgccctcc
     1681 agaccctgct cgaacagatc ccgcgggaca acctcaaaca ggcctgccag accctgcagg
     1741 acggaggttt ctggaagaat atgcccacgg ccgaaaagga cctcaagtcc tttgtcttca
     1801 ccctggccct cgaccccgat gactccgaca agaacaaggc ccgcgccgcc cgcctcttct
     1861 ccaccggcaa cgccaacagt atgtacaacg aactcgctca aaacttcgaa aagaaataat
     1921 atgaaaaccc ttctccgcat tctgtgcctg ctcgtactaa ccacgacagc cgcacaggca
     1981 caaacaaaag tcttcaaggc cgtcgccgac gatatgtccc aggacttcga acccatcata
     2041 caggacggaa acctcgtcgg ctacctggta tttacccaat tggaaagagc cagcgccgac
     2101 tcttttaatt atagcatcga gatcatggat gagaacctga atgatatcgg cacggttaaa
     2161 ttccgcgaag agaaactgaa cctgaaagcg gtttccttcg accaggatgt cctctgcctg
     2221 gcctacgtaa aaaccaattt cgtgggcagg gaattcaaga acgaaaagga atttcgcaat
     2281 ttcaaaacgg agggtaaggc agccctcttt acacagtttc tcggcctcaa cggcaagatc
     2341 ctcgcctcca acagtacaaa aatgcagatc acgcccgatt tcgcctacgc cgctaacagc
     2401 aaccggaggg tcatcggcaa cggtaaacta aaacacggga tcctgttgcg aaacatctcc
     2461 ggcaccggct tcgcatgctt ctatggcgac gactccaaaa acaacctgat cgtcttcaac
     2521 accacaggca agctggtctg gcaaaaacag ataaaggaaa ccggcgacga ctttaccatg
     2581 cttacctccg gccccgaagt atccctcctc gtcaagatga gcgaccagat gaaagagggc
     2641 ggctaccagg ttcttagcta caacgccgtc gacagtaccg cctatcccaa attcctcctg
     2701 aaggataaga aaggcaattc cctgaaagta ctggccttcg aaaacgatcc cgtgtccggc
     2761 aaaccctatc tcgccggcct ggtcatcgac cccattcgcg gcaaccacta tggctacggc
     2821 agggcgatct cccacggccc ctactgcggc cttttcacga tcaacctaaa cggccataca
     2881 aaaaaggaca tccaggcctc tttctcctac tggaatgacg ggtcccaaaa ctttatcgac
     2941 aaatacggct acctgccgca ggcccacgaa tacgccaata tcgagcgcgc cttcagggac
     3001 tatcagggca ataccatctt tgccgcctgc ggcatcacca gcaaggtccg ctggggcgcc
     3061 atcaccggcg cagtcctcac cgtctggacg atctacggcg cgcccgcctt cctcgccggc
     3121 ggcaccagca aatatgccac ccgcgatatc ctcctgatga aacaggacgc cgccggcaaa
     3181 ctcgatctgg ccaccaccgt tccggccccg agatcctcct acagcttcgc cacctttccc
     3241 gtctcatctt atgatccgaa caactactat acggtaaaga attccgacac ccgcacccag
     3301 tatctcgtca tcgacaaggc aaaggacatc gacatctata acgtcaatca gaaaaaggtc
     3361 gcccgcacga ttccacataa agagggtaat aatctggtca ccgtcttccc cgccaaggaa
     3421 ggctatgtga tggtctacga attcaataaa aaagaaaaaa ccacccgcct gtcgatcgaa
     3481 gcactataaa gatttgtatc ttcagagtat gagaacccta acggccctca tcctcttact
     3541 tactgcatcc tttgcatacc cccagccggc ccttacaagc cggctggatt cttagtcccc
     3601 tacagtctga tctcttcttt cccgatggtc aaaaattagc tccaaaacct tgtaaggccg
     3661 gtcccggttt gtacttacca taccccgcca atcctctcac ctccttcacc tgcgtgttcc
     3721 ccgggtcccg gaattgcgga tccccgtata tcgccggcgc gccctccgca gcgtcggcag
     3781 ccacccggtc atccccatgc aatacccacc acgcatttgc cttgaacaga tcccccatcc
     3841 atcgctgacc caggatcaaa ctatccttcc ctacaaaaat attatggtga agtacaaacc
     3901 cccggtgcgc tcttctccga aaaactgacg gccgccacct tcgtattgta gatcgtattg
     3961 ttatacacga cacaatcgtg aaactgcaga ctgtccccgc tgctgttcca gatatacagg
     4021 ccggcatgcg aatccgacac cgcaccatcg tcctcgctta tattgtaccg gaaaatatta
     4081 tgataccacg gactggctcc ccaatactgg aagagacaat agccggcgcc ctggttgcca
     4141 taggagagac aatattgaac gatggaatgg gtcacccctc catccaggtc gaacccgcca
     4201 ccgtctgcgc cacccaccga agtcttgtta cgataggaaa gacaatgctg aataacgaca
     4261 ctatcggatt cataacacca gatcccgaca ggcccgttgc cgatcctcgg catgtcccag
     4321 ccattgttcg tcgcagcaca acggtcgatc agcaggttgg tacaatgacc ggcgacaatg
     4381 ccatttccgc tgtgattggt gagattggtc gggtcgcctg gattgtcgtc cgcccggcaa
     4441 tcgacgatag tgatattccg gcaggaaagc ttgccctcgc ttccctccac cgtgatcccg
     4501 gccgacccgt tctcatgcgc aaagaccctg ctgatcacca ccccctttga ggcatagatc
     4561 agcaccccgg acttttgaaa gcccgtaaca tcgatagcat cgaccctcac ctgcccgcat
     4621 tcgataagcc gcaatccatc cctgacattg ccggtcttcc ggcccgaacc caccagccgc
     4681 aatcccttta gccggaccca gtccgcccgg tagaacacca ccgccgaaga atcgcccgcc
     4741 ggaagggtcg ccgccccctg tccgtacacc ccgatccagg caggatgcgc agcactcccg
     4801 ccctcacctt ccacccgcag caccccggta aaggattgcc ctccccgaaa aaagacacta
     4861 tcgccgggct gtaaccgcag ccggttgaca cgatccatcg tcttccaggg atgagccctg
     4921 ccgccatcgc cgccgtcatc accgctgttg tcgacataat aattcctccc tgctacggtc
     4981 aaccggccaa ggagaaaaaa gaccatcagc agggccgccg gcccggaaat aaatcttctc
     5041 atagatcaat agcccgtatt tggttcaatg tagttattta atacacccgt tgtatcaaaa
     5101 ccggacatcc tcctctttgc gcgcatctta aacccttgat cctccgccac ctgctatccg
     5161 gcattttctt tggtcgaaat tcagcacaat tcattcatat gaagggaacc atcgcccaaa
     5221 gccaattcct ccggctcaat cgcatcaccg gctggatcat cggcctcatc gcgtgcacgg
     5281 tctatatcct taccatggaa cccaccggca gcttctggga ctgcggcga
//
LOCUS       DATGFG010000790         5818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_624570, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000790 DATGFG010000000
VERSION     DATGFG010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624570"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VKQ52_14825"
     CDS             <1..320
                     /locus_tag="VKQ52_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88525.1"
                     /translation="SQPISMAFSVKFTSTLTGTISDLDVLIQGELVSSPGYKVALTED
                     YTTHGVLTVKYQVSGDNGTDFTITYACTTAGTDAKDPGQPSPVKGTIVKNGYTELTLT
                     IDV"
     gene            331..1383
                     /locus_tag="VKQ52_14830"
     CDS             331..1383
                     /locus_tag="VKQ52_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88526.1"
                     /translation="MRCLRFRPIYTLALCLAFFFPLALSAQDYFNDRAATGTPILLGK
                     DTHAALFSASGSIGDNSVKANFFKQYWLNAATYKADRLPRVKNYLGWGISGKASTVNG
                     LGALFSLGDFSPSFNTGAYLSFSQKSWRASGDGTVYGNWALILSGNFTTCYYNIYTPR
                     AAFSHQLVDTPFHALGVALSFVDCIYKGQDDLYVGASVSVKRQNNYSFLYKVTIRNDT
                     AYTTPGSGGSSGTTRTVTAINTNGDTYGVGPYFQYTDVGVRLNLSWVPASLQHDVAFI
                     LYPSMDLSRAYTPVYNLGTSIAWLKTGSPSTPLAAVFFELDDVGNAYGSIYTFGKRSF
                     RAGISASLNILTGVLK"
     gene            1650..1832
                     /locus_tag="VKQ52_14835"
     CDS             1650..1832
                     /locus_tag="VKQ52_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88527.1"
                     /translation="MKIKDPIEEFVLLRAMKLTRLILFIGFLQVSAVTTAQRLPISIK
                     NSALEKWFSEIEKKTD"
     gene            1887..2135
                     /locus_tag="VKQ52_14840"
     CDS             1887..2135
                     /locus_tag="VKQ52_14840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ88528.1"
                     /translation="MKDASVEEVLQVSLNGLGVSGVVQPESGIPLAGVTVSIKKIKKL
                     GVTHEVGKFDPKDVPNGEYEVKISYVGLVDESSGRDRG"
     gene            2599..2811
                     /locus_tag="VKQ52_14845"
     CDS             2599..2811
                     /locus_tag="VKQ52_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88529.1"
                     /translation="MGHDPLHQLAKGANTILHRNSFHAIIVTIILIGEAQHHYPIESL
                     NSSLSSAESTFALVLGAIIRSLFDDE"
     gene            2898..3023
                     /locus_tag="VKQ52_14850"
     CDS             2898..3023
                     /locus_tag="VKQ52_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88530.1"
                     /translation="MATAKKAAKKKVAKKAVKKAAPKKAAKKVAKKAVKKAAKKK"
     gene            complement(3094..>5818)
                     /locus_tag="VKQ52_14855"
     CDS             complement(3094..>5818)
                     /locus_tag="VKQ52_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HLZ88531.1"
                     /translation="PIVITLLLLLIVSPLIKLSIMGPAEKVRLRDFNGYCFALVAGSM
                     VLTLIVIQVLLLTDDDMRQDQHLHLLSGKVDSSFRQEIGHAYRQLVAMDTLPLRPDSH
                     GVVRDWRKGYFDLTTELVDYMRQHRDSAYYNFDRIDWVTDSGRQVINGSLDRNLDTLF
                     IDVSKRKYFTDFKDNSSYTLPGVDTALVSIQPVVTWTEGASRIVIAKRSRVPGIFIVT
                     LSTDLYSLNRTILPSGFGFCLIDGDGRVQVHSDPIHSVNENFIDEVGDAAQFRSAMKA
                     RQELYLPSTKFYGREYGLIVKPVPNLPYFLVTYYDKGYDQPVNMRILIFSLAGCGMEL
                     LLLGAMWLLFFRRRWNERPLLFGVIDYLPWVVPRVTAARIYRFGWMALLIYVVVTTLA
                     VVLSVFYGPGNNQRVLALLLATPVLLTIVLKAIIRMRRDRIRKSIPVSHGEDEILLVP
                     EEPFEAETPLDHLLHYCLLVEALVLAMGVLPAGLFTWYANNQELLQSVKKEQFLLAGA
                     LDSRKPVLYQNLQALDSGVAPKDLYADWQYHRGLYPLNREQLTVGRDTMPPPETAFGV
                     ASTYFEVAGWLANDYYDPEYVPVLVGGSDDGQWRWTKPRADTMRFYYEQNRNSVLQVA
                     SVMPQRYYYLSDWRKGLLLLALVLALLYGLYHLIRRVTAGMFLQKFVMGESERRLPYF
                     DAYSDQESLAGDEKDVRARAMREEIVRDLEPWGKDTELDVMELETLDCIRRWTDYFTF
                     VLDGCNAKEKYLLYQFACNGFLNYKNVVEIDDLLHRGVLVVENEEVKLFSRAFRAYIL
                     THVREDELEKSFIRRSPWQRFRNPFLVLLMIAAAFLFFTRQEAWQRISALIAALSTSL
                     GLLSGLFREGVASGWRGGSSGGGVGGSAGREGGGSAEGGAGDGGSASGSGE"
BASE COUNT         1392 a         1774 c         1443 g         1209 t
ORIGIN      
        1 cttctcaacc tatctctatg gcattttccg tgaaattcac ctccacgctc accggcacca
       61 tctccgacct ggacgttttg atccaggggg aactcgtctc ctcgccggga tacaaggtcg
      121 ctctcaccga ggactacacg acgcacggcg tcctcaccgt caaataccag gtgtccgggg
      181 acaacggcac ggatttcacc atcacctatg cgtgtacgac ggccggcact gacgccaaag
      241 accccggtca accctcgccc gtaaaaggaa ccattgttaa gaacggctac accgaactaa
      301 cgctaaccat cgacgtttaa accttccgct atgcgctgtc tacgcttccg cccaatctac
      361 acgctggccc tatgccttgc gttctttttc cccctcgccc tctccgccca ggactacttc
      421 aacgaccgcg ccgcgacggg caccccgatc ctcctcggca aggacaccca tgccgcgctt
      481 ttctccgcga gcggcagcat cggcgacaat agcgtaaagg cgaacttctt caaacaatac
      541 tggctcaacg ccgccaccta caaggccgac cggctgcccc gcgtcaagaa ctatctcggc
      601 tggggcatct cgggtaaggc cagcacggtc aacgggcttg gcgccttgtt cagcctcggc
      661 gatttcagcc cttccttcaa cacgggggcc tatctttcgt tttcgcagaa atcctggagg
      721 gcttcgggcg atggaacggt gtacggcaac tgggccctga tcctttccgg caatttcacc
      781 acctgctatt ataatatcta tactccacgt gcggcgttca gccaccagct agtggatacg
      841 cctttccatg cgctgggtgt cgcgctgtcc ttcgtcgatt gcatctacaa gggacaggac
      901 gacctctatg tgggcgcctc agtctcggtg aaacggcaga acaactattc cttcctctac
      961 aaggtgacga tccgtaacga caccgcctac accacaccgg gcagcggcgg cagcagcggt
     1021 accacccgca cagtgacggc catcaacacc aacggcgaca cctatggcgt cggtccctat
     1081 tttcaataca ccgatgtcgg cgttcgtctc aacctctcct gggtgcccgc ctctcttcag
     1141 cacgatgtcg ccttcattct ctatccttcc atggatctct cgcgtgccta cactcccgtc
     1201 tataatctcg gcacctccat cgcctggtta aagaccggca gcccctcgac gccgctggcc
     1261 gccgtcttct tcgaactcga cgacgtcggc aatgcgtacg gcagcatcta cactttcggc
     1321 aaacgctcct tccgcgccgg catctctgct tccctgaata tcctaaccgg cgtgttgaaa
     1381 taggcggagc agccgttttg ggcccgcgaa gcaatttgtc aattttggca caatgggcga
     1441 gggttggatt tccgcaaagg ataaaattca cctaaatcaa tttgcttttc ccggtttata
     1501 caatagattg gcataggtat cgtgtaaatc cccaatttca ttctgttaat cttcacttta
     1561 gcgcggcaac aagcaattga tttacaatac accgttttac ttaatcgttc gtcactagtt
     1621 aaagaacaac tattcgacgt acaatttgta tgaaaataaa agatccgatc gaggaatttg
     1681 tcctgctacg cgccatgaaa ctaaccaggt taatcctctt tatcggtttt ctgcaggtga
     1741 gtgcggtaac caccgcgcag cggttgccca tttccattaa aaacagcgcg ctggagaaat
     1801 ggttctccga gatcgaaaag aagaccgact aaacattttt ttatgatgcc acgatccttc
     1861 agcatgcgaa gccgataacg gtggagatga aggatgcctc ggtggaagag gtgttgcagg
     1921 tttcgctgaa cggccttggc gtgtcgggtg tggttcagcc cgagtcggga atcccgctgg
     1981 ctggggtcac ggtgtcgatc aagaagataa agaagttggg tgtgacccat gaggtgggga
     2041 aatttgaccc gaaggatgtg cctaatgggg agtatgaggt gaagatatcg tatgtgggat
     2101 tagtcgatga gtcatctgga cgagaccgtg gttaaggggt attattcaac gacaaaccga
     2161 ttgaacacag gagatgtggc gacggtgaag ggggaggaca tccagataag gggtcagaat
     2221 agtattgcaa gtgggaatga tcccctatat atcattgatg gggtgccttt cgagcgctgg
     2281 aacttattct tggtatggag gattgagccc atttaatgcc ctaaatcctg cccaaatcga
     2341 aagcgttgag gtgcttaaag atgcagacgc gacagcgatt tatggatcaa gggtggcaaa
     2401 cggggctatt ttggttacta ctaaaaaagg caaaatggga gatataaatt ttcgtgtggt
     2461 cgcaaataga ggtgccggaa caaccacgca caaattgcac attttgaata caccacaata
     2521 cctggaaatg cgacgggagg cttttaaaaa tgacggctca ccggcctcag caacacctac
     2581 gcgtgaaatt attgtcgctt gggacacgac ccgctacacc aactggcaaa aggcgctaac
     2641 acgatactcc atcgaaactc cttccacgcc atcatcgtaa cgatcatcct catcggggaa
     2701 gcgcagcatc attaccctat cgaatcactc aactcctcgt taagctccgc cgaaagcacc
     2761 ttcgccctgg tgctcggcgc cataatccgc agcctcttcg atgatgaata acacatataa
     2821 caccaatttt ctttgttcga attattgatt tccactatct ttgtataagt atttactaac
     2881 caattaaatt caaaaacatg gcaacagcaa aaaaagcagc caagaagaaa gtcgctaaga
     2941 aagcagtaaa gaaagcagcc cccaagaaag ctgcaaagaa agtagccaag aaggcagtta
     3001 agaaagccgc taagaaaaag taaataagaa agccccctct ccgggggctt ttccttttat
     3061 atccttcgcc gatcgcaatt ctacttctcg cttttattca cccgatccac tcgccgatcc
     3121 tccatcaccc gccccacctt ccgccgaacc accaccttcc ctccccgcgg agccgccaac
     3181 gccgccacca ctactacccc cacgccaacc cgaagccacc ccctccctaa acaacccact
     3241 caataacccc aacgacgtac tcaacgccgc gatcagcgcg gagatccgct gccaggcctc
     3301 ctgccgcgta aagaagagga aggccgccgc gatcatcagc aacacaagga aaggattgcg
     3361 gaatcgctgc cagggactcc gccttataaa cgacttctcc agctcatcct cccgcacatg
     3421 cgtcaggata tacgcgcgga acgcccggct gaagagcttc acctcctcat tctccaccac
     3481 cagcaccccg cgatgcagca ggtcatctat ctccaccaca ttcttataat tcaggaaccc
     3541 attgcacgca aactggtaca gcaggtattt ctccttcgca ttgcacccgt ccagcacgaa
     3601 ggtgaaatag tcggtccatc gccggataca gtccagggtc tcgagctcca tcacatcgag
     3661 ttcggtatcc ttgccccagg gctccaggtc ccgcacgatc tcttcccgca tcgcccgcgc
     3721 acgtacatcc ttctcgtctc ccgccagcga ctcctgatcg ctgtaggcat cgaagtacgg
     3781 cagccgccgc tccgattccc ccatcacgaa cttctgcagg aacatccccg ccgtcacccg
     3841 gcggataaga tgatacagtc catataagag cgccaacacc agcgccagca gcagcagccc
     3901 cttgcgccag tccgaaagat aatagtatcg ctgcggcatc accgacgcca cctgcaggac
     3961 actgttccgg ttctgctcat aataaaaccg catcgtgtcc gcgcgtggtt tcgtccaccg
     4021 ccactgtccg tcatcactcc cgcctaccag cacgggaaca tattccgggt cgtaatagtc
     4081 attggccagc cagcccgcca cctcgaaata cgtcgacgcc accccaaagg cagtctccgg
     4141 cggaggcatc gtgtcgcggc ccacggtaag ctgttcgcgg ttcagcgggt agaggccccg
     4201 gtgatactgc cagtcggcat agaggtcctt tggcgccacg ccgctgtcga gggcctggag
     4261 gttctgatag agcaccggct tacggctatc cagcgcaccc gcgaggagga actgttcctt
     4321 tttaacagat tgcaggagct cctggttatt ggcataccag gtgaagagtc ccgcgggcag
     4381 tacccccatg gccaggacga gggcctcgac gaggagacaa tagtggagca ggtgatcgag
     4441 gggtgtctcc gcttcgaagg gttcctcggg cacgagcagg atctcgtctt ccccatggga
     4501 tacggggatc gactttcgta tcctatctcg ccgcatgcgg atgatcgcct tgagcacgat
     4561 ggtcagcagc acgggtgtcg ccagcagcag ggccagcacg cgttggttgt tgcccggtcc
     4621 gtaaaaaaca gaaaggacca cggccagcgt cgtcaccacg acatagatca gcagggccat
     4681 ccagccaaag cggtagatcc tcgccgccgt cacgcgcggg acgacccagg gcaaataatc
     4741 gatcacgcca aagagcaggg gccgctcatt ccagcgccgg cggaagaaga gcagccacat
     4801 ggcgcccagg agcagcaact ccatcccaca gcccgccagc gaaaagatca ggatgcgcat
     4861 attcacgggc tggtcatatc ccttatcata ataggtgacg agaaaatacg ggagattggg
     4921 cacaggcttc acgatcaacc catactcgcg gccatagaat ttcgtactgg ggaggtagag
     4981 ttcctgccgc gccttcatgg ccgagcgaaa ctgggcggca tcccccacct cgtcgataaa
     5041 attctcattg acggaatgaa tcgggtcaga atgcacctgc acccgcccat ctccatcgat
     5101 gaggcaaaac ccaaacccgg agggcaggat cgtccggttg agtgaataca gatcggtgga
     5161 aagcgtcacg ataaagatcc ccggcacccg gctccgcttg gcgatcacga tccgcgacgc
     5221 cccctccgtc caggtgacca cgggctgaat gctcaccagc gccgtatcca cccccggcag
     5281 ggtatagctg ctgttatctt tgaagtcggt gaagtatttc cgcttcgaca catcgatgaa
     5341 gagggtgtcg aggttacgat cgagactgcc gttgatgact tgtctaccac tatccgttac
     5401 ccaatcgatc cggtcgaaat tataataggc gctgtcgcgg tgctgccgca tatagtcgac
     5461 cagctccgtc gtcagatcga aatagccctt gcgccagtcc cgcaccaccc catggctgtc
     5521 gggccgcagg ggcagggtat ccatcgccac cagctgccgg taggcatgcc cgatctcctg
     5581 gcggaagctg ctgtccactt tccccgagag aagatgcagg tgctgatcct gtcgcatatc
     5641 gtcatccgtc agcaacagca cctggatgac gatcagcgtg agcaccatgc tccccgccac
     5701 cagggcgaag caatacccgt tgaagtcgcg cagccgtacc ttctccgccg gccccatgat
     5761 cgaaagcttg atcagcggtg aaacgatcag cagcagcaac agtgtgatca ctataggg
//
LOCUS       DATGFG010000791         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_204544, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000791 DATGFG010000000
VERSION     DATGFG010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204544"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VKQ52_14860"
     CDS             <1..1171
                     /locus_tag="VKQ52_14860"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLZ88532.1"
                     /translation="LAKGGVFGPYLDGGNYTMAKLVDEKTLPDSVRARHILIATVDAR
                     TGQVINDDSVARKRIDSIKTLIDNGGNWDSLALKLSDDPGSKTKGGDLGYFTGDRMVK
                     EFADFCFSGKKGEKKIVKSQFGYHYIEILDQKNFEPAYKVAYLSRKIDVSQETDQAAS
                     GLASQFAGESRDAKAFEANVQKEHLQKMFAPDIPPSESSIPGLGVNRQLVKWAYDADL
                     GNVSEPYAVGDKYVVALVTGIDKEGTMLPNKARNQIEPILRNKKKAEQIIRKLGSPAS
                     LDAASTAAGQPVLHADSVLFSSPFIPNAGQEPKVIGAAFDKQLAGKPVSPAIPGNGGV
                     YFIKVENVSAVSNPNADVQQQRFMQEQQQRQRVAYGLFDAMRKLATVKDYRAKFF"
     gene            1200..1706
                     /locus_tag="VKQ52_14865"
     CDS             1200..1706
                     /locus_tag="VKQ52_14865"
                     /inference="COORDINATES: protein motif:HMM:NF022118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HLZ88533.1"
                     /translation="MPEEIVNKVAQSGLYTLDLEEYYPKEEIVVFDLKPLLFMEMILK
                     EKEFRAALQSVDWTRYQDKILAVTCSVDAIIPSWAYMLVAVQAQPYVKDLFLGDRQTV
                     LQEIFLANLRAIDITEFSDKRVVVKGCGDLSVGGFAYLEIARRLRPVVKSILYGEACS
                     NVPIFKKK"
     gene            1723..>2087
                     /locus_tag="VKQ52_14870"
     CDS             1723..>2087
                     /locus_tag="VKQ52_14870"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HLZ88534.1"
                     /translation="MDVEILARVQFAFTIGFHYIYPPLSIGLGLMLVILEGLYLRTGN
                     RIYEMAVRFWIRIFALIFGIGVATGIIMEFEFGTNWATYSKYVGDIFGSALAAEGIFA
                     FALESGFLGILIFGWNRVGP"
BASE COUNT          510 a          596 c          555 g          426 t
ORIGIN      
        1 cctcgcgaaa ggcggcgtct tcggacccta tcttgacggt ggcaactata cgatggcaaa
       61 gctggtcgat gaaaagaccc tgcccgattc cgttcgcgcc cgccatatcc tgatcgctac
      121 cgtcgatgcc cgtacaggac aggtcatcaa tgacgacagc gtagccagga aaaggatcga
      181 cagcatcaag accctgatcg acaacggtgg taactgggac agcctggcgc tgaaattatc
      241 ggatgacccc ggttcgaaga caaagggcgg cgacctgggt tacttcaccg gcgaccgcat
      301 ggtaaaggaa tttgccgatt tctgtttcag cgggaagaaa ggcgagaaaa agatcgtaaa
      361 aagccagttc ggctaccatt atattgagat tctcgatcag aagaatttcg agcccgccta
      421 taaggtggcc tacctcagcc ggaagatcga cgtaagccag gaaacagacc aggcggcatc
      481 cggtctcgcc agccagttcg caggcgaaag cagggatgca aaagcatttg aagccaatgt
      541 tcaaaaagaa catctgcaaa agatgttcgc ccccgatatc ccgccgtccg aatcctctat
      601 ccccggactg ggcgtcaatc gccaattggt caaatgggcc tacgatgccg acctcggaaa
      661 tgtctccgaa ccgtatgcgg ttggcgataa atatgtggtt gccctggtga ccgggatcga
      721 caaggaaggg accatgcttc ccaacaaggc aaggaaccag atcgaaccca ttctgcgcaa
      781 taagaaaaaa gccgagcaga tcatcaggaa actgggcagc cccgcatcgc tcgacgccgc
      841 atccaccgcc gccggccagc cggtactgca cgccgacagc gtccttttct cctcgccctt
      901 tatccccaac gcgggccagg aaccgaaggt gatcggcgcg gcattcgaca aacaactggc
      961 gggtaaaccg gtctcccccg cgatcccggg taatggcggt gtatacttca taaaagtaga
     1021 gaacgtaagc gcggtttcca acccgaacgc cgatgttcag cagcagcgct ttatgcagga
     1081 acagcagcag cggcaacggg tcgcctatgg cctgttcgac gcaatgcgaa aactggcgac
     1141 cgtaaaagac tacagggcaa aattcttcta aaaagagttt cccgtcgtat ctttgcatcg
     1201 tgcctgaaga gatcgtcaat aaagtagcgc aaagcggcct gtatacgctg gatctggaag
     1261 agtactatcc gaaagaagag atcgtcgtct tcgacctgaa gccgcttttg ttcatggaga
     1321 tgatcctgaa ggaaaaggaa ttccgcgccg ccctccaatc cgtggactgg acccggtacc
     1381 aggacaagat cctggccgtc acctgctccg tcgatgcgat cattccatcc tgggcgtata
     1441 tgctggtagc cgtgcaggca cagccctatg tgaaagactt gttcctggga gaccgccaga
     1501 cggtcctgca ggaaattttt ctggccaacc tccgggccat cgatattacc gaattttccg
     1561 acaaaagagt agtggtaaaa ggatgcggcg atctgtccgt cggcggattt gcgtacctcg
     1621 agatcgcccg caggctgcgg cccgtcgtca agagcattct ttatggggaa gcctgtagca
     1681 acgtccccat attcaagaag aagtaacaca catactcata ctatggatgt cgagatcctg
     1741 gccagagtgc aattcgcttt tacgatcggg tttcactata tctatccccc gctcagcatc
     1801 ggtctgggcc tcatgctcgt tatcctcgaa ggcctctacc tgcgcaccgg caaccgcatc
     1861 tatgaaatgg ccgtccgttt ctggatccgc atcttcgccc tcatcttcgg catcggcgta
     1921 gccaccggca tcatcatgga attcgagttc ggcaccaact gggccaccta ctcgaaatat
     1981 gtgggcgata tcttcggcag cgccctggcg gcggaaggca tctttgcctt tgccctcgaa
     2041 tcgggattcc tgggtatcct catcttcggg tggaaccgcg tcggccc
//
LOCUS       DATGFG010000792         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_629954, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000792 DATGFG010000000
VERSION     DATGFG010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629954"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VKQ52_14875"
     CDS             <1..264
                     /locus_tag="VKQ52_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88535.1"
                     /translation="CWAKTRFHYLDLTVAKFNAGGETTKVLTDEAFTNDFVRNVLSYF
                     SLSPFDKLIDNTGFCQYNRLREVQKKRNIIRYGINTIRRKIFG"
     gene            269..1396
                     /locus_tag="VKQ52_14880"
     CDS             269..1396
                     /locus_tag="VKQ52_14880"
                     /inference="COORDINATES: protein motif:HMM:NF025665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HLZ88536.1"
                     /translation="MEIRPVAIYLPQFHPIAENDEWWGKGFTEWTNVTKAKPLLKDHY
                     QPHLPADLGFYDLRLADAREEQARLAREYDVYGFCYYHYWFNGHRLLNRPVDEVLASG
                     KPDLPFMFCWANENWSRRWNEEDQAILMKHEYSFEDDEQHINFLMPFFKDPRYIKVGN
                     KPVFAIYRADLFPDMKKTIGIWRRVARENGFDDIYMLTVQYFQHTFVDPKDQGFDAAI
                     SFQPNWTKAPSKLFGSFKDKLLHKLKIRESLYRHNYVTTYREFSQRMMSLPAPGYKAY
                     PCVTPGWDNSARKRRRGAILLGSTPQLFAAWLADILRKFKPWSKEENFIFINAWNEWA
                     EGNHLEPCQKWGRAYLEALKETLREAREVGEAAQPINTTQQ"
     gene            1398..2270
                     /locus_tag="VKQ52_14885"
     CDS             1398..2270
                     /locus_tag="VKQ52_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLZ88537.1"
                     /translation="MRGKIKQLYHRFIKFQPGLHYDYRVSDYRIYVDHFKGRAGIEIG
                     GPSRMFEYFGSLPLYPLVSGLDGVNFSGNTVWEGNINPGKTFAFDERKRLGYQYIGEA
                     ADLSFVPDGKYDFLISSNCLEHLANPLKAIKEWLRVIRPDGVLLIVVPHKDMTFDHHR
                     PVTTFEHLLEDEKNNTGEDDKTHLEEILRLHDLQKDPAAGTPDQFRDRCGKNMDNRCM
                     HHHVFSVSLLSRIFQHLDKQVIHLDVLNDGNIVVLVRKHEILVPDTQLLTATLFKSPF
                     KTDRDDLRYRMLHQ"
     gene            2239..3108
                     /locus_tag="VKQ52_14890"
     CDS             2239..3108
                     /locus_tag="VKQ52_14890"
                     /inference="COORDINATES: protein motif:HMM:NF025089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ88538.1"
                     /translation="MISVIVCSINESARSAFAGELAATIGVPYELILVKNEEEKLSIC
                     AAYNKGASRAKYPFFCFVHEDLTFHTPGWGAGLVRCLETPGAGMLGFAGSTMKTHSPS
                     PWWITHEDPLALPFRRLHYIQSGIRQSRPAESEQLLQEVLCLDGFFLACSKTTWEITG
                     FDERTLRGFHFYDMDISLSAHAKGLKNYVYTGCWVEHHSSGSLNIDWVRGAEAFQRKW
                     RGRLPLSLQPVPAGEQKELERLALVDYLYVLVKTGCRWLWLKYFFRLIRKYGNSPSAR
                     MVGKVYLKQFLPS"
     gene            3105..>3566
                     /locus_tag="VKQ52_14895"
     CDS             3105..>3566
                     /locus_tag="VKQ52_14895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ88539.1"
                     /translation="MSTTGDTGSISVIIPVKNGATTIGQCLEALFGQSVRQRMEVILI
                     DSGSTDGTLEIAAGFPVKIVRIEPAGFDHGLTRNLAVGHATGDLVYLTVQDARLADAR
                     ALERMAAWFRDPAVMAVCGIQGVPHEKDTNPVYWYRPVSAPTVDRLQFPGPD"
BASE COUNT          800 a          967 c         1041 g          758 t
ORIGIN      
        1 tgctgggcca agacgagatt tcactacctc gatctgacgg tggcgaaatt caacgcaggc
       61 ggagagacga caaaggtgct gacggatgag gcgttcacca acgattttgt ccggaacgtc
      121 ctttcttatt tcagcctctc cccctttgac aagctgatcg acaacaccgg gttttgtcaa
      181 tacaaccggc tgagggaggt tcaaaagaaa aggaacatca tccgatacgg aatcaatact
      241 atcagacgaa aaatatttgg ttaagaaaat ggagatccga cctgtcgcaa tctatcttcc
      301 ccagttccac ccgatagccg agaacgacga atggtggggg aagggtttta cggaatggac
      361 gaatgtcacg aaggcgaagc cgttattgaa ggaccattac cagccgcatc tgccggcaga
      421 cctcgggttc tatgacctgc ggctggcgga tgcgcgcgaa gagcaggccc ggctggcgcg
      481 ggaatacgat gtctatgggt tttgttatta tcactactgg ttcaacgggc accgcctgct
      541 gaaccggccc gtcgatgagg tgctggcttc cggcaaaccg gatctgccct ttatgttctg
      601 ctgggccaat gagaactggt cgcggcgctg gaacgaagag gaccaggcca tcctgatgaa
      661 acacgagtac agcttcgagg acgacgagca gcatatcaac ttcctgatgc cctttttcaa
      721 ggacccgcgc tatatcaagg tgggtaacaa accggtattc gctatttacc gcgcggacct
      781 ttttcccgac atgaagaaga ccatcggcat ctggcggagg gtcgcccgcg aaaatgggtt
      841 cgacgatatc tatatgctga ccgttcagta tttccagcat acgttcgtgg acccgaagga
      901 ccagggcttc gatgccgcca tcagtttcca gcccaactgg accaaggccc cgtcgaagct
      961 tttcggctcc ttcaaggata agctgctgca caagctgaag ataagggaat ccttgtaccg
     1021 gcataattat gtcaccacct accgggagtt ttcgcaacgg atgatgtccc ttcccgcgcc
     1081 cggctacaag gcctatccct gtgtgacccc cggatgggac aacagcgcgc ggaagagaag
     1141 aaggggggcc atcctgctcg ggtcgacccc gcagctgttt gccgcgtggc tggccgatat
     1201 cctgcgcaag ttcaagccct ggtcgaagga agagaatttc atctttatca atgcctggaa
     1261 tgaatgggcc gaaggcaatc acctcgagcc ctgtcagaaa tggggccggg cctacctgga
     1321 ggccctgaag gaaaccctgc gggaggcgcg ggaggtcggc gaggcggctc aaccgataaa
     1381 cacaacccaa caatagcatg cgcgggaaga tcaaacaact gtatcaccgg ttcatcaaat
     1441 tccagcccgg gttgcattat gactaccggg tgtcggatta ccgcatttat gtcgatcact
     1501 tcaagggaag ggccggcatc gagatcggcg ggcccagccg gatgttcgaa tacttcggca
     1561 gcctgccgct ctacccgctg gtcagcggac tggatggggt caatttttcg gggaacacgg
     1621 tctgggaagg gaatatcaac ccggggaaga cctttgcttt cgatgagaga aagcggcttg
     1681 ggtaccaata tatcggtgag gcggccgatc tgtcctttgt gccggacggg aagtatgatt
     1741 ttcttatctc ttccaactgc ctggagcacc tggccaaccc gctaaaggcg ataaaggaat
     1801 ggctgcgggt catacggccc gatggcgtcc tgctgatcgt cgtgccgcac aaggatatga
     1861 cctttgatca ccaccggccc gtcaccacct tcgaacacct gctggaagat gagaagaaca
     1921 acaccggcga ggacgataag acgcacctgg aggagatcct gcgattgcac gacctgcaaa
     1981 aagatcctgc cgcgggcacc ccggaccaat tcagggaccg ctgcggaaag aatatggaca
     2041 accgctgtat gcatcatcat gttttcagtg tcagcctgct ttcgcggatc ttccagcatc
     2101 tcgacaagca ggtgatccac ctggatgtgc tgaatgacgg gaatatcgtg gtgctggtcc
     2161 gcaaacatga gatccttgtc ccggatacgc aactgctgac cgcgaccctg ttcaagagtc
     2221 cctttaaaac agaccgagat gatctccgtt atcgtatgct ccatcaatga gtccgcgagg
     2281 agtgcttttg ccggtgagct cgcagcaacg atcggtgtcc catacgagtt gatcctggtg
     2341 aagaatgagg aagagaagct gtccatctgc gcggcctata acaaaggagc gtcaagggca
     2401 aaatatcctt tcttctgctt tgtacatgaa gatctcacct ttcatactcc cggctgggga
     2461 gcaggcctgg tacggtgcct ggaaacaccc ggcgccggga tgctgggctt tgccgggtcg
     2521 acgatgaaga cccattctcc ttctccctgg tggatcacgc atgaggatcc gctggctctc
     2581 ccttttcgcc ggcttcatta tatccaatcg ggcatccggc aatcccggcc cgctgaaagc
     2641 gaacagcttt tacaggaggt gctttgcctg gacgggttct ttctcgcctg ttcgaagacc
     2701 acctgggaga tcacggggtt tgacgagcga acgctgcggg gctttcattt ttatgatatg
     2761 gatatcagcc tgtcggcgca tgcaaaggga ttaaagaact atgtatacac ggggtgttgg
     2821 gttgaacatc attcttccgg ttccctgaat atcgattggg taagaggggc cgaggccttt
     2881 cagcggaaat ggaggggcag gttgccgctc agcctgcagc cggtgcccgc cggcgagcag
     2941 aaggagctgg aacggctggc ccttgtagac tatttatatg tgctggtgaa aaccggctgc
     3001 cggtggttat ggctaaagta ttttttccgg ctgatccgca aatatggcaa ttccccctcg
     3061 gccaggatgg tgggtaaggt ttacctgaag caattccttc catcatgagc acgacagggg
     3121 acaccggatc gataagcgtc atcatcccgg tgaagaacgg ggctaccacg atcggccagt
     3181 gcctggaggc cttgttcggg cagagcgtac ggcagcgaat ggaagtgatc ctcatcgatt
     3241 cgggttccac ggacggaacc ctggagatcg ccgccggctt cccggtaaag atcgtccgga
     3301 tcgagcccgc cggctttgat cacgggctta cccgtaacct ggcggtcggt catgccacgg
     3361 gtgatcttgt gtatctcacc gtccaggatg cgcggctggc cgatgcacgg gcgctggaaa
     3421 ggatggcggc ctggttccgg gacccggccg tcatggccgt ctgcgggatc cagggtgtcc
     3481 cgcatgagaa ggataccaat cccgtctact ggtaccggcc cgtgtcggcg ccgacggtcg
     3541 accggctgca attccccggg ccggac
//
LOCUS       DATGFG010000793         5909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1614015, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000793 DATGFG010000000
VERSION     DATGFG010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614015"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VKQ52_14900"
     CDS             complement(<1..297)
                     /locus_tag="VKQ52_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ88540.1"
                     /translation="MPLSASALTRLRYQHQTIRELIGSLPEHQLRQRINPDKWSAFEN
                     IAHLACYQPVFHYRLERIQQDNAPSFSRYVAEADPAFPGYTQQQLGRLLGGIDAD"
     gene            complement(311..1342)
                     /locus_tag="VKQ52_14905"
     CDS             complement(311..1342)
                     /locus_tag="VKQ52_14905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HLZ88541.1"
                     /translation="MPDFNRNDTINLLSKLTARRIFNGMKVLGSYYLSKWTKKAIQWG
                     YPVSLSFEPTTSCNLRCPECPSGLRAFTRPTGMLENGFFRQTIDQLSKDLLYLIFYFQ
                     GEPYLNPSFLDMVAYASSKGIYTATSTNAHYLNDRNAKRTVESGLDRLIISIDGTTQE
                     VYEQYRVGGHLDKVLEGARNIVKWKKELKSRTPFIFFQFLVVRPNEHQLADIRRLAKE
                     IGVDQVRFKTAQVYDYENDPNGLIPVNERYSRYRKEKDGHTVFKNSLDNHCWRLWHAT
                     VITWDGLVVPCCFDKDAEHPLGDLKGKPFREVWHNEQYVEFRRQILQSRKNIDICANC
                     SEGTRVWGN"
     gene            1480..2181
                     /locus_tag="VKQ52_14910"
     CDS             1480..2181
                     /locus_tag="VKQ52_14910"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HLZ88542.1"
                     /translation="MLGFTAYDWWLERRAHFVRYPEFGIDIPVNYSIHGIDVSKYQEI
                     IDWSSVRDMKVGNVQMSFAFIKATEGLGNEDAYFKRNWKKVRDAGLARGAYHFFLATK
                     SGKAQAENFINSVELEPGDLPPVLDIEQTYGISPDKLRDRAREWLQTVQDYYHVMPII
                     YTNVDFYKQYLKDDFDGYPLWVAHYLQKERPSIYRAWAFWQHSEGGRVNGILTKVDFD
                     VFNGDSTEFRKLLIN"
     gene            complement(2200..2808)
                     /locus_tag="VKQ52_14915"
     CDS             complement(2200..2808)
                     /locus_tag="VKQ52_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88543.1"
                     /translation="MELETLKSIWQDQEFPDNQPARDELLALLHKKSHGPINRMRRNL
                     RKELLLMIFSYTFCIIFYLTAFNGEFSGVSWLFVGVLLYFGIYYNQKNRLLKKMQCAS
                     CEVRSNLEGQLRTLKKYLRFYYWSGVLSAPITIIIAFGLAYNSRLSNDPPFHWWRAIG
                     LLSALIIATTVATYFVSRWAIEKFYGRHVRKLQELVREMDEA"
     gene            complement(2789..3310)
                     /locus_tag="VKQ52_14920"
     CDS             complement(2789..3310)
                     /locus_tag="VKQ52_14920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ88544.1"
                     /translation="MEYLALQEAFIRLVNDNRGILYKICRMYGATDADRQDLFQEIVV
                     QLWRSYPSFRGESKFSTWLYRIALNTAISDLRKQRSYITPVDPDHLPTELQDIQYNTE
                     KEEQLQSLYAAINRLTEVEKALTMLYLEDKPYAEMEEILGIPQNNLRVKMNRIKEKLR
                     KMTKEAVYGVGNA"
     gene            3377..3730
                     /locus_tag="VKQ52_14925"
     CDS             3377..3730
                     /locus_tag="VKQ52_14925"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HLZ88545.1"
                     /translation="MKKIYHLSTCTTCQRILEETGATGKGFTLQDIKTDRITAAELDG
                     LKEKVGSYEALFSRRAIKYKELGLKDQVLTEADYRRLILEEYTFLKRPVAVSGKKVFV
                     GSEKKTVEELKKSIG"
     gene            complement(3719..4315)
                     /locus_tag="VKQ52_14930"
     CDS             complement(3719..4315)
                     /locus_tag="VKQ52_14930"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLZ88546.1"
                     /translation="MHSNDKPLIPLDSCYFLTLNTVDKIDVFTRPAYKQVIADTLNYF
                     VETQGLAVYSWCLMTSHLHLIVRTKEAAAPAYFEREFKKHTTLTILKTIETEMDFRRD
                     WMLQHFEDYRKSLQRKEKFHLWQDCSSPLRIDCKQPRMLFDRVMHIHENPVRERIVEQ
                     PEAYLFSSARDYIGRPGLVRVRVVQQQGLFGTKLLSSN"
     gene            complement(4429..5001)
                     /locus_tag="VKQ52_14935"
     CDS             complement(4429..5001)
                     /locus_tag="VKQ52_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88547.1"
                     /translation="MTEMYKIQPKGLYFVTLTVVGSIDVFTRYEYCDLLVENLNYCID
                     NKRLRVYEYAILPSQLYMIADVELGKGNLPKVLRDLKSYSAKQILRAISEHPDESRKE
                     WLMRLFHFFANRYQHDSEHHFWQFGNQPVDLEKIVKKDKPIPSPIDKLMKAKWVDDPG
                     HYIYCSAYPLQKVKLSTVNGLIHEPHIEKK"
     gene            complement(5186..>5909)
                     /locus_tag="VKQ52_14940"
     CDS             complement(5186..>5909)
                     /locus_tag="VKQ52_14940"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLZ88548.1"
                     /translation="NYLQQVSLVTDADEKDPNADTVKLMSIHAAKGLEFTCVFAAGLE
                     ENLFPNAMSINTREELEEERRLFYVVITRAKQKLWITYANTRYRFGNLVQNEPSRFIE
                     EIPSDYLDRSFAGGGARNQGYAGSSGSAYDRMHGGGFGNSAAQAEKKYGPPPAKKDQR
                     PSYVPPKPYMKTIEHNPSPDFVASDTSGLQVGQKVEHQKFGFGEVIGMEGNTHNPIAT
                     VKFQYNGEKKIMLNFAKLRIVG"
BASE COUNT         1293 a         1388 c         1700 g         1528 t
ORIGIN      
        1 gtcggcgtcg atgccgccga gcagccgccc cagctgctgc tgtgtgtagc cggggaaggc
       61 ggggtcggcc tctgctacat agcggctgaa ggagggtgcg ttgtcctgtt ggatgcgttc
      121 gagccggtaa tgaaataccg gttgataaca ggccaggtgg gcgatgttct cgaaggcgct
      181 ccatttgtcg gggttgatgc gttgtcgtag ctgatgctcc ggcagggaac cgatcagctc
      241 ccggatggtt tggtgctggt atcgcagacg ggtcagggca gaagcggata aaggcatacg
      301 attcgttttt ttaatttccc cacactcttg ttccctcgct gcagttggcg caaatatcga
      361 tattcttccg gctttggagg atctggcgtc tgaactccac atattgctcg ttatgccaaa
      421 cctccctgaa cggttttccc ttcaggtcac cgagcgggtg ttcggcgtcc ttgtcaaagc
      481 agcagggaac caccaggccg tcccaggtga tgaccgtggc atgccacagt cgccagcagt
      541 gattgtccag ggagttctta aaaacggtgt ggccgtcctt ttctttccgg tagcggctgt
      601 atctttcgtt gacggggatg aggccgttgg gatcgttctc atagtcatac acctgggccg
      661 ttttaaaacg cacctggtcg accccgatct cctttgccag ccggcggata tccgccaact
      721 gatgttcgtt tggccggacg acgaggaact ggaagaagat gaacggtgtc ctgctcttca
      781 actctttttt ccatttgacg atattccggg cgccttcgag cactttgtcc aggtggccgc
      841 cgacgcggta ttgttcgtag acctcctggg tggtgccgtc gatggaaatg atcagccggt
      901 cgagtccgct ttccactgtc ctttttgcgt ttcggtcgtt caggtaatgt gcatttgtgg
      961 atgtggcggt gtagatgccc ttcgaggagg catatgccac catatcgaga aaggaggggt
     1021 tgagataggg ttcgccctgg aaataaaaga tgaggtagag cagatctttc gacaactggt
     1081 cgatggtctg gcggaagaag ccgttctcca gcatgccggt gggccgggta aaagcgcgca
     1141 acccgcttgg gcattcggga cagcgaaggt tacaggaggt ggtgggttcg aaggagagcg
     1201 agaccgggta tccccactgg atcgcttttt tcgtccactt gctcagataa tagctgccca
     1261 ggaccttcat cccgttgaag atccgacggg cggtaagctt ggagaggaga ttgatggtat
     1321 cgttacgatt aaaatcgggc atggaagtat aaaaatacta aattatgttt gtaaccgttt
     1381 ggacaccccg agaggcttct catcgctatg gtaaaaaaga ataagaaaaa tacaggttgg
     1441 aaaattgtaa tattctctgt cattgcagcc gtgcttgtta tgctgggttt tacggcttat
     1501 gactggtggc tggaaagaag ggcgcatttt gtccggtatc ccgagttcgg tatcgatatt
     1561 cccgtcaatt acagtattca cggcatcgat gtttccaaat accaggagat catcgactgg
     1621 agttcggtgc gggatatgaa ggtcgggaat gtccagatga gttttgcctt tataaaggcg
     1681 acggaggggc ttggcaacga ggatgcgtat ttcaaacgga actggaaaaa ggtgagggac
     1741 gccggtctgg cccgtggcgc ctatcatttt ttcctcgcga ccaagagtgg caaggcgcag
     1801 gcggagaatt tcatcaactc cgtggagctg gagccggggg atcttccgcc ggtgctggat
     1861 atcgagcaga cctatggcat ctccccggat aagttgcggg acagggccag ggagtggcta
     1921 cagacggtgc aggactatta tcatgtgatg ccgatcatct ataccaatgt agacttctat
     1981 aagcagtacc tgaaggacga ttttgacggc tacccgcttt gggtggcgca ttacctgcaa
     2041 aaggagcggc cgagcatcta tcgcgcctgg gctttctggc agcatagcga ggggggccgg
     2101 gtgaacggca tcctgacgaa ggtggacttc gatgtattca atggggattc caccgaattc
     2161 cggaagctgc tgatcaatta aatgggccag cccgtcggcc tatgcctcgt ccatttcccg
     2221 gaccagttcc tgtagtttcc ggacatgccg cccatagaac ttttcgatgg cccatcgact
     2281 gacaaagtag gtggcgaccg tggtagcaat gatcagggcc gataggagtc cgatcgcccg
     2341 ccaccagtga aaaggcgggt catttgacaa ccggctgtta tatgcgagcc caaaagcgat
     2401 aataatggtt atcggtgcgc tcaggacgcc agaccagtaa tagaagcgaa ggtatttttt
     2461 caatgtcctc aactgacctt ccaggttgga acggacctcg cagctggcac attgcatttt
     2521 tttgagcagc cgattcttct gattataata aatgccgaag taaagaagga cgcctacaaa
     2581 cagccaggat acgccggaaa actcgccgtt aaaggccgtc aggtagaaga tgatgcagaa
     2641 agtataggaa aagatcatca gcagtagttc tttccgcagg ttccggcgca tccggttgat
     2701 cgggccgtgc gacttcttgt gcagcaatgc cagcagttcg tcccgtgcgg gttggttatc
     2761 agggaactcc tggtcctgcc agatggattt aagcgtttcc aactccataa acggcctcct
     2821 ttgtcatttt gcgtagtttc tctttgatac ggttcatttt gacccgcagg ttattctgcg
     2881 ggataccgag gatctcttcc atttccgcat agggtttgtc ttccaggtaa agcatggtca
     2941 gggctttttc cacctccgtc aaccggttga tggcggcata gagggactgc aactgttctt
     3001 ctttttccgt attgtattgg atgtcctgca attcggtggg caggtggtcc gggtctacgg
     3061 gggtaatata gctgcgttgt ttgcgcagat cggaaatggc ggtattcagg gctatccggt
     3121 aaagccaggt gctgaatttc gactcgcccc ggaaggaggg ataggatcgc cagagctgga
     3181 cgacgatctc ctggaacagg tcttgccggt cggcatcggt ggcgccgtac atcctgcaga
     3241 tcttatagag gatgccgcgg ttatcgttca ccagccggat aaatgcctct tgcagggcca
     3301 ggtattccac attcgaggtt ttgacaataa tactactatc tgtcgtcggt tggtggaaaa
     3361 tattacattc gcgtccatga agaagatcta tcatctctcc acctgcacca cctgccagcg
     3421 gatcctggag gagacgggtg cgaccggaaa ggggtttacc ctgcaggata tcaagacgga
     3481 caggatcacc gcggccgagc tggacggatt aaaggaaaaa gtggggtctt atgaggcgtt
     3541 gtttagccgg agggccatca aatacaagga attaggcctg aaagaccagg ttttgaccga
     3601 ggccgattac cgccggttga tcctggagga gtatacgttc ctgaagcgcc cggtggcggt
     3661 gtcgggtaaa aaggtctttg tgggcagtga gaagaagacg gtcgaggagc tgaaaaagtc
     3721 aattggatga cagcagcttc gttccgaaga ggccctgttg ctggacgacc cttaccctga
     3781 ccaggccagg tctccctata tagtccctgg cgctgctgaa caggtaggct tccggctgtt
     3841 cgacgatcct ttccctgacg gggttctcgt ggatatgcat gacccggtcg aacagcatcc
     3901 ggggttgttt gcagtcgatg cgcagggggc tgctacagtc ctgccagaga tggaatttct
     3961 ccttgcgttg cagggatttg cgataatctt caaagtgctg gagcatccag tcgcggcgga
     4021 agtccatctc tgtttcgatc gttttcagga tggtgagggt ggtatgtttc ttgaattccc
     4081 gctcgaaata ggccggggcg gccgcttctt ttgtccggac gatcagatgc aggtggctgg
     4141 tcatcaaaca ccaggaatag accgccaggc cctgggtctc tacaaaatag ttcagggtgt
     4201 cggcgatcac ctgcttataa gccggccggg taaagacatc gattttatct acagtattca
     4261 gtgtcaggaa ataacaggaa tccagcggga tgagcggctt atcgttggaa tgcataggct
     4321 cttattttgg atgacaaaat aatagggtgc aaacaggtat aggctgggga tgtttctaaa
     4381 attacaaaaa tcccgctgaa tcagttgcga agcgccaaaa atttatcctt attttttttc
     4441 gatgtggggt tcatggatca ggccgttcac cgtggagagt tttacctttt gcagggggta
     4501 ggcgctgcaa tagatataat gcccggggtc gtcgacccat tttgccttca tgagcttatc
     4561 gatggggctg gggatgggtt tgtctttttt aacgatcttt tccaaatcga ccggctggtt
     4621 gccgaactgc cagaaatggt gttccgaatc gtgctggtac cggttggcga agaaatggaa
     4681 caggcgcata agccactcct tccggctttc atcggggtgt tccgaaatgg cgcggaggat
     4741 ctgtttggct gaataacttt tgagatcgcg caataccttt ggtaaattcc ctttgcccag
     4801 ctccacatcc gctatcatat acaactggct gggcaggata gcatattcgt atacccgtaa
     4861 ccgtttgtta tcgatgcagt agttgaggtt ctctaccaac agatcgcagt attcatagcg
     4921 tgtgaatacg tcgatgctgc cgactacggt tagggttaca aaatacaatc ccttcggctg
     4981 tatcttgtac atttcggtca tgcgtaaaag atagcgaact tttgttaaaa tgaataactg
     5041 cgcgggtttt cagaagatgg caagaaggct ttttttgttt cactgcgatg aaaccgttgg
     5101 ctttcgttcc caccaggaga atctttccac gcaggagaag ttttccaatc atttcctgca
     5161 aaaaaattgc attcagtgca tccggctacc cgacgatccg aagcttagcg aagttgagca
     5221 tgatcttctt ttctccgttg tactggaatt tgacggtcgc gatcgggttg tgtgtgttgc
     5281 cttccattcc gataacttcg ccaaatccga acttctgatg ttctactttt tgtccgacct
     5341 gcaggcctga ggtgtcgctt gctacaaaat cgggagaggg attgtgttcg atcgttttca
     5401 tatatggctt gggcgggaca taggaaggtc gttgatcttt ttttgcgggc ggagggccat
     5461 atttcttttc cgcctgtgcg gcgctattgc caaagccgcc gccatgcatc cggtcgtagg
     5521 cggacccgga tgatccggca tatccctggt tgcgtgcgcc gccaccggca aagctgcggt
     5581 ctaaataatc ggaggggatc tcttcaataa aacggctggg ttcattttgt accaggttgc
     5641 caaaccggta ccgggtgttt gcataggtga tccacaattt ttgtttcgcc cgggtgatca
     5701 cgacataaaa cagtcttctt tcctcttcga gctcttccct tgtattgatg gacatcgcat
     5761 tggggaagag gttctcctcg aggcccgcgg caaagacgca ggtgaactcg aggcctttcg
     5821 ccgcatggat gctcatcagc tttacggtgt cggcgttagg gtccttctca tcggcgtcgg
     5881 tgaccaggga gacctgttgc aggtagtta
//
LOCUS       DATGFG010000794         7960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1309642, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000794 DATGFG010000000
VERSION     DATGFG010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309642"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..967
                     /locus_tag="VKQ52_14945"
     CDS             497..967
                     /locus_tag="VKQ52_14945"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HLZ88549.1"
                     /translation="MAVKLNLDKLDLQIIHEMMENAEISYAELGKKLFVSGGTIHVRI
                     KKLQELSVVKGTKLHVDLKQLGYDVIAFIGIYLEKSSLYDSVARELQKIPEIVRLNYT
                     TGNYSMFAEIVCKDINQLRFVLHDELQKIKGIERTETFISLDESINRNVQVFKE"
     gene            complement(1004..2065)
                     /locus_tag="VKQ52_14950"
     CDS             complement(1004..2065)
                     /locus_tag="VKQ52_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88550.1"
                     /translation="MKGKRNIGRRLLRNKGAFFGLVVIAAAIIVAVTAYFIAPDGSPD
                     ANRIIVEIGGRKPGFRQQFLLLKKQAPAQPVSFLHRLLYGREDRYDYVPINGYATQGD
                     SIIVQKYVDEGVFERRAYPVAAALQIKTQRFWLGTDKYGRDILSRLLIGVRVSLAVGM
                     VTVLLSLSVGVLLGALAGYFRGRIDEAILWLVNVIWSIPTLLLVFAIMLVLGKGFWQV
                     FVAIGLTLWVNVARIVRGQVLAVREREYVEAARALGYSHDRILFRHILPNIMGPVWVV
                     AASNFASAIVIEAGLSFLGVGVQPPQPSWGLMIKENYNFIITHNPMLALAPGMAIMLL
                     VLAFNLLGNGLRDVLNVRV"
     gene            complement(2062..3297)
                     /locus_tag="VKQ52_14955"
     CDS             complement(2062..3297)
                     /locus_tag="VKQ52_14955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ88551.1"
                     /translation="MPLSFLLSLVGCLFFAVYGGLIAWYHRAWTGIPVFVPCRTEGYQ
                     TRITVLVPARNEEENIGNCLQSLAAQSYPRGLFEVIVIDDHSTDDTAAIVKEFAVAGP
                     LAGTTLARPLADSAVADAPLAVRYLPLAEEPRQGTLTAHKKFAIETGVARATGELIVT
                     TDADCLFDPDWLLTLAACYEENGAKFIAAPVRIGGMSSWGRQHSLLSVFQTLDFITLQ
                     GITGAAVYKRVHSMCNGANLAYAKEAFLEVGGFQGIDQIPSGDDMLLMHKIFLRYPDR
                     VFFLKRRQAIVSTRPETRWKGFFHQRVRWASKADRYDDKRIFWVLLLVYLVNGLFLLS
                     AIAACWDRWWGFLCLMLLVVKTLVEYPFVSEVAAFFGQRKLMAWFPALQPLHIGYTLV
                     VGWLGKFGSYRWKDRIISK"
     gene            complement(3303..4130)
                     /locus_tag="VKQ52_14960"
     CDS             complement(3303..4130)
                     /locus_tag="VKQ52_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLZ88552.1"
                     /translation="MSEHFIPFRSSRFHYCTWGTGSRLLFAFHGYGESAATFAFLGDA
                     LGNDFTIIAPDLPFHGKTEWNEDRHFLDPRDLLSLMAEIAAAFPGREAGWWLLGYSMG
                     GRVALQLLELAPEKVRRLVLIAPDGLIVNSWYWLATQTRVGNALFHRAMRSPGWLFLL
                     MRTGNRLQLVNPSLYKFAVHYIDDDAARRSLYTRWTTMRGFRPRLGPIAAIIRDRQIP
                     VVLLYGSYDRIIRSERGEHFRQRIAPWGRLTLLSAGHQLLQPKFMEVIANALYLPII"
     gene            4309..4749
                     /locus_tag="VKQ52_14965"
     CDS             4309..4749
                     /locus_tag="VKQ52_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLZ88553.1"
                     /translation="MPNNQFRKGELYSFITGKASTAIARNLQKKFNAAGLKITIEQWS
                     VLYHLWKEDGMSQQELCSATFRDKPSITRLVDNLEKQQLVKRVPSESDRRINLIYLTK
                     QALKLQEETMQLAEATLNEALEGVPGDRIDICKEVLQIVYDNLR"
     gene            4771..6564
                     /locus_tag="VKQ52_14970"
     CDS             4771..6564
                     /locus_tag="VKQ52_14970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLZ88554.1"
                     /translation="MSASTEKKQALKGGEWLIKEGSPFLTFIPEDFNEEQQMVKDMCN
                     AFLDAEVLPVIDRIDKLEPGLMPTLIQKAGEQGLLGAAVPEEYGGLGKDFVTATLVNE
                     GLGGGFSFSVGVSAHTGIGTLPILYFGTPEQKKKYIPKLASGEWKGSYGLTEPNSGSD
                     ALGAKTTAKLSAAGKYYLLNGQKCWITNGGFADIYTVFAKIDGDKFSAFIIERGFEGF
                     TQGPEEHKMGIKGSSTVQLYFQDCKVPVENLLGEIGKGHIIAFNILNIGRLKLCAAAA
                     GGAKRAATTSIAYAKTREQFKTPIANFGAIKHKIAEMAIRIWAAESALYRTTKWIDEK
                     EVGLAAEGKPFNEAVLGAAEEYAIECAILKVFGSEMLDFVVDEGVQIHGGNGYSDEYI
                     ISKAYRDSRINRIYEGTNEINRLLTVDMVLKRAMKGRLDIMGPAMAVSKELMSIPEFG
                     NDGDTPFAKEKKTIQNFKKAILMTAGAAVQKLMMRLESEQEILMDIADMAIETFIAES
                     ALLRAWKLTDQRGAAGAAYELDIMRTYLYDAADRINKSGKDAINAFADGDEQRMMLLG
                     LKRFTKVDPFNSKAARRRIADKLITDNKYPL"
     gene            6689..7879
                     /locus_tag="VKQ52_14975"
     CDS             6689..7879
                     /locus_tag="VKQ52_14975"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLZ88555.1"
                     /translation="MRRSTIGLLFLLLTYRGFSQDSLRLARTTGQLPFMEYGIGDDRL
                     GGAKMGFLDSNVLVKIVDSFNTDYKVRLSAQHYAYIAKTSVALQGKQSGRPIAHNPHL
                     SGSIKVFGDTASDYVALTVDDRYPYRSMQLLDPSRVVVDVFGVTSNTNWITQLHTARE
                     IRNTWYEQIEDDVLRIYIQLKHPQHWGYALAYDSAGRHLVIRIRRQPPVLDIRKLRIA
                     IDAGHGGDNSGADGVTSKVLEKNYTLLIAEQLRKTLKAAGVQHIFMTRTKDTSLSMPE
                     RIAMLKQYDPHLLVSIHLNSAGSDTVQGTSTYYRYIGFRPLSLAILERMLTLGLKEYG
                     NVGSFNFSLSGPTDYPNALVEVAFLSNREDEKRILNPKFHKAVAQKIYLGIMDWLNQQ
                     KPKP"
BASE COUNT         1939 a         2126 c         2269 g         1626 t
ORIGIN      
        1 tcagcgggtg gtcgttcctg aaaaaagagc cccatttacc cgccatccct tcgggatatt
       61 gcagcacatt gggaaaggat ttgatcgctt cgaaacggat gagttttttt tggcccatga
      121 agcggcaaaa atacaaaaaa aggccccgcc gaaggcgggg ccttaaaatg aatactgctg
      181 tagaggaagg attcgaacct ccacggagaa gttagcgcta gcgcaatgtg cggtggtcaa
      241 ccccagtcga acccgatttt ggtcggatcc ggcattacac tatgtttatc cctgattctc
      301 cacccccgag acaagagggc atgtctgcca agtttcatca ctccacaatg gtaaaagaac
      361 ttcgtgaatc gttgttgaaa caaaattaaa gaaatggccg aattttttac attttttgaa
      421 caaaaatttt tgtgttttca aaattattca aatatttgca atatagtata gataaaattc
      481 aaaaactcta ttcaagatgg ctgttaaatt gaatctcgac aaattagatc tgcagatcat
      541 ccatgaaatg atggaaaatg cagagatctc ctacgcggaa ttggggaaaa agctatttgt
      601 ttcgggaggt acgatccatg tcaggattaa gaagctgcag gaactctcgg ttgttaaagg
      661 tacaaaattg catgtcgatc tgaaacaact gggctatgat gtcatcgctt ttatcggtat
      721 ttacctcgag aaaagctccc tttatgacag cgtggcaaga gagctgcaga agatccccga
      781 gatcgtgcgt ctcaactaca ccaccggcaa ctacagtatg tttgcggaga tcgtctgcaa
      841 agacatcaac cagttgcgct ttgtccttca tgatgaattg caaaagataa agggcatcga
      901 gcgcacggag acctttatct ccctcgacga aagtattaac cgcaacgtcc aggtcttcaa
      961 agaatagccg ctccccgcct ttcgctttcc tgtcactttc cactcacaca cgtacattca
     1021 aaacatccct gagtccgttt cccaggaggt taaacgccag caccagcagc atgatcgcca
     1081 tgccgggtgc gagcgccagc atcggattgt gggtaatgat gaagttatag ttctccttta
     1141 tcatcaaccc ccagctgggc tgtggcggct gtacgcctac gccgaggaaa ctcagaccgg
     1201 cttcgatgac gatcgccgat gcaaaattgg atgcggcgac gacccatacg ggccccatta
     1261 tattgggaag gatatgcctg aagaggatac ggtcatgcga atagccgagg gccctcgccg
     1321 cttccacata ttcccgttcg cgcacggcca gcacctggcc gcggacgatc ctagccacat
     1381 tcacccatag tgtcagcccg atggccacga acacctgcca gaagcccttc cccagcacca
     1441 gcatgatcgc aaacaccagc agcagtgtag ggatgctcca gatgacattc accagccaga
     1501 ggatggcttc gtcgatgcga ccgcggaaat agcccgccag cgctcccaac agcacgccta
     1561 cactcaggga aagcagcacg gtcaccattc ccacggcaag gcttaccctg acgccgatga
     1621 gcaatctgct gaggatatcg cggccatatt tgtccgtgcc cagccagaag cgctgtgtct
     1681 ttatttgcag ggcggcggcg acgggatagg ccctcctttc gaagacgccc tcgtccacat
     1741 atttctgtac gatgatgctg tcgccctggg tggcatagcc gtttatcggg acataatcgt
     1801 acctgtcttc gcggccgtag agcaggcgat gaaggaagga taccggctgc gcgggcgcct
     1861 gttttttcaa caacaggaat tgttgccgga agccgggttt gcggccgccg atctcgacga
     1921 tgatacggtt ggcatcgggt gagccgtcgg gggcgataaa ataagccgtg acggccacga
     1981 tgatggcggc tgcaataacg acgagcccga aaaaggcccc cttattgcgc agcaaacgcc
     2041 ttcctatatt tcgttttccc ttcatttgct tattattctg tccttccagc gatagcttcc
     2101 gaactttccc aaccagccga cgacaagcgt atagccgatg tggaggggct ggagtgccgg
     2161 gaaccaggcc atcagtttgc gctggccgaa aaaggcggct acctcgctga cgaacggata
     2221 ttccaccagg gtctttacga ccagcagcat gaggcataag aatccccacc agcggtccca
     2281 gcaggcggcg atagccgata gcaaaaacaa cccgttgacg agatagacca gcaacaacac
     2341 ccagaagatg cgtttgtcgt cataacgatc ggccttgctc gcccagcgga cgcgctgatg
     2401 aaaaaagccc ttccaccggg tctcgggccg cgtggagacg atggcctgac ggcgttttaa
     2461 gaagaacacc cggtcgggat agcgaaggaa gatcttatgc atcagcagca tgtcgtcgcc
     2521 cgaggggatc tgatcgatgc cctggaatcc tcctacctcc aaaaatgcct ccttcgcata
     2581 cgcgaggttg gcgccgttgc acatggaatg gacgcgttta tagaccgcgg cgccggtgat
     2641 gccctggagg gtgataaagt ccagggtctg gaagacggaa agcaacgaat gctgccggcc
     2701 ccagctgctc atcccgccga tccggacggg cgcggcgatg aattttgccc cgttttcctc
     2761 ataacaggcc gccagcgtca gaagccagtc cggatcgaag aggcaatccg cgtcggtcgt
     2821 gacgatcagt tctcccgtgg cccgggccac accggtttcg atggcgaatt tcttgtgcgc
     2881 ggtgagggtg ccctgacggg gttcctccgc aagaggaagg tagcggaccg cgagtggtgc
     2941 atcggcgacg gctgagtccg cgagcggcct ggcgagagtg gttccggcga gcggcccggc
     3001 gacggcgaat tcctttacga tagctgcggt gtcgtcggtg gaatggtcat cgataacgat
     3061 cacctcgaag agtccgcggg gataactttg agcggcgagg ctttgcaggc aattgccgat
     3121 gttctcctct tcgttgcggg cgggcaccaa taccgtgatc ctcgtttgat aaccttccgt
     3181 ccggcagggg acaaagaccg gaatgcccgt ccaagcccgg tgataccagg cgatgagccc
     3241 gccgtatacg gcgaagaaca gacaaccgac gagcgaaagc aggaatgaca acggcatggt
     3301 cctcaaatga tcggtaaata tagcgcgtta gcaatgactt ccatgaattt aggttgcagc
     3361 aactggtgcc ccgccgacag cagggtcagc ctgccccagg gggcgatgcg ctgccggaaa
     3421 tgttcgccac gttccgaccg gatgatgcgg tcatagctac cgtagagtag aacgacgggg
     3481 atctggcggt ctcggatgat ggcggctatc gggcccagcc ggggccggaa accgcgcatg
     3541 gtggtccagc gggtatagag actccggcgc gccgcatcgt catcgatgta atggaccgcg
     3601 aatttgtaca ggcttggatt taccagctgc aaccggttgc ccgtccgcat cagcaggaag
     3661 agccagcctg gtgatctcat cgcccggtgg aacaacgcat tgcccacccg cgtctgcgtg
     3721 gccagccaat accaggagtt tacgatcagc ccgtcggggg cgatcagcac cagccgccgg
     3781 accttttccg gcgccagttc gagcaattgg agggctaccc ttccgcccat gctatacccc
     3841 agcagccacc atcccgcttc ccggccgggg aaggcggcgg cgatctccgc catgagggaa
     3901 agaaggtcgc gggggtcgag aaaatgccgg tcttcgttcc attccgtctt tccatgaaag
     3961 ggcaggtcag gagcgatgat cgtgaagtcg ttgccgagcg catctcccag gaacgcaaag
     4021 gtggctgcag actccccata cccgtggaag gcaaaaagga gacggcttcc cgtcccccag
     4081 gtacaatagt gaaaacggga cgaccggaag ggtatgaaat gctcagacat ggactaaatg
     4141 tacgcgaata gtgctggtct gcaatgtaac cctattcgcg taaaaggcta aagtctggcc
     4201 ttcggccgat ccgcctccgg cggatccggt acaatcaaaa tatttctcca aaattctttc
     4261 gatataaggg aaaaatgcgt atttttattt agttgtttaa ctaactgtat gccaaacaac
     4321 caattcagaa aaggagaact ctacagtttt ataaccggta aggcgagtac ggcgatcgcg
     4381 cggaacctgc aaaagaagtt caatgctgcc gggctgaaga tcacgatcga gcagtggagc
     4441 gtcctttatc atctttggaa agaggatggc atgagtcagc aggagctttg cagcgctacc
     4501 ttccgggaca agccaagcat cacccgcctg gtggataacc tggaaaaaca gcaactggtg
     4561 aagcgcgtac cttcggagag cgaccggcgg atcaacctca tttatcttac gaagcaggcg
     4621 ttgaagttgc aggaggagac gatgcaactg gccgaagcca ccctcaatga ggcgctggag
     4681 ggcgtacctg gcgaccgcat cgatatctgc aaggaagttt tgcaaattgt atacgacaac
     4741 cttcgataac tcatcttaaa actattttct atgagcgcgt ctactgaaaa aaaacaagcc
     4801 cttaaggggg gcgaatggct gataaaggag gggtctccct ttttgacctt catccccgaa
     4861 gatttcaacg aagagcagca gatggtaaaa gacatgtgca atgccttcct ggatgccgaa
     4921 gttctgccgg tgatcgaccg tatcgacaag ctcgagccgg ggctgatgcc tacgcttatc
     4981 cagaaagcgg gtgagcaggg acttcttggc gcggccgtcc ccgaagaata tggcggcctg
     5041 gggaaggact ttgtcaccgc gacgctggtg aatgaagggc tgggcggcgg tttttccttt
     5101 tctgtaggcg tctccgcgca tacgggtatc ggtacgctgc cgatcctcta tttcggcaca
     5161 cccgagcaga agaagaaata tatccccaag ctggccagcg gggaatggaa gggctcctat
     5221 gggctgaccg agcccaacag cggcagcgat gccctggggg ccaagacgac ggcgaagctc
     5281 tccgccgccg gcaaatacta tttgctcaac ggccagaaat gctggatcac caatggcggt
     5341 tttgcggata tctataccgt ttttgcgaaa atagacggcg acaagttctc cgcatttatc
     5401 atcgagcggg ggttcgaggg atttacgcag gggccggaag agcacaagat ggggatcaag
     5461 gggtcctcga cggtgcaact gtatttccag gattgcaagg tcccggtgga gaacctgctg
     5521 ggagagatcg ggaaggggca tatcatcgcg tttaatatcc tgaatatcgg tcgcctcaaa
     5581 ctttgcgcgg ccgcggccgg gggtgcgaag cgggcggcca ccacctccat cgcgtatgcg
     5641 aagacccgtg agcagttcaa gacgccgatc gccaattttg gcgccatcaa gcacaagatc
     5701 gccgaaatgg ctatccgcat ctgggcggcc gagagcgcgc tttaccggac gacgaaatgg
     5761 atcgatgaaa aggaagtcgg cctggccgcg gagggcaagc cgttcaacga ggcggtactc
     5821 ggcgccgccg aggaatatgc catcgaatgc gccatcctca aagtctttgg cagtgagatg
     5881 ctcgatttcg tggtcgacga aggggtgcag atccatggcg gcaacgggta cagtgatgaa
     5941 tatatcatct ccaaggccta tcgcgacagc cggatcaatc ggatctatga gggaacgaat
     6001 gagatcaacc gattgctcac ggtagatatg gtgctgaaac gggcgatgaa gggaaggctg
     6061 gatatcatgg ggccggccat ggccgtgtcg aaggagctga tgagcatccc cgaattcggc
     6121 aatgatgggg atacgccctt tgcaaaggaa aagaagacga tccagaattt taaaaaggcg
     6181 atcctgatga cggccggcgc cgcggtgcag aagctgatga tgaggctcga gagcgagcag
     6241 gagatcctga tggatatcgc cgatatggcc atcgagacct ttatagcgga gagcgccctg
     6301 ctgagggcct ggaagcttac cgatcagcgg ggagcggccg gcgccgccta cgagctcgat
     6361 atcatgcgca cgtatcttta tgatgccgcc gaccggatca acaaatccgg gaaggacgcc
     6421 atcaacgcct ttgctgacgg cgacgaacag cggatgatgc tgctggggtt gaaacgcttt
     6481 accaaggtcg atcctttcaa cagcaaggcc gcgagacgcc gtatcgccga caagctgatc
     6541 accgataata aatatccgct ataacagcga tgaacgagcc gataacccgg aagacttgcg
     6601 ggtccacaca tgagcaacga agccaccacg caaatttttt gcgggtggct tcctcttttg
     6661 cgagtatctt taataaaaga aattgcgaat gcggcgatca acgattggcc tcctcttcct
     6721 gctgctgacc tatagaggtt tttcacagga cagcctgcgg ctggcgcgga cgacgggaca
     6781 actacccttt atggaatacg gtatcggcga cgaccggctt gggggtgcga agatgggttt
     6841 cctggattcg aatgtgctgg tgaagatcgt cgacagcttt aacaccgact acaaggtcag
     6901 gctctccgcc cagcattatg cctatatcgc caagaccagc gtggccctgc agggtaaaca
     6961 atccggcaga ccgatcgctc acaacccgca tctctccggt tccataaaag tatttggcga
     7021 cacggcctcc gactatgtcg cgcttaccgt ggatgatcgt tatccttatc gcagcatgca
     7081 gcttctcgac ccgtcgcggg tagtggtaga tgtcttcggt gtgaccagca atacgaactg
     7141 gatcacgcag ctgcatacgg caagggagat ccgcaacacc tggtacgagc agatcgagga
     7201 cgatgttctc cgcatctata tccagctgaa acatccgcaa cattgggggt atgcgcttgc
     7261 ctatgacagc gcgggccggc atctcgtcat ccggatccgg cggcagccgc ccgttctcga
     7321 tatccggaaa ctgcgtatcg ccatcgatgc gggtcatggc ggggataatt ccggcgccga
     7381 tggcgtaacc agcaaggtgc tggagaagaa ctataccctg ctgatcgcgg aacagcttag
     7441 aaaaacgcta aaggcggcgg gggtgcagca tatttttatg acgcgtacaa aggatacttc
     7501 gctgagcatg ccggagcgga tcgcgatgtt aaagcagtac gatccacacc tgctggtgag
     7561 catccatctc aactccgccg gttcggacac cgtccaggga acgagcacct attaccggta
     7621 tatcgggttc cggccgctga gcctggccat cctggaaagg atgctgacgc ttggtttaaa
     7681 ggagtatggg aatgtgggca gcttcaattt ttcgcttagc ggccccaccg actatcccaa
     7741 tgcgctggtg gaggttgcct ttctcagcaa ccgggaggat gaaaagcgga tcctcaatcc
     7801 aaaattccat aaggcggtgg cgcagaagat ctacctgggc atcatggact ggctgaacca
     7861 acaaaaaccc aaaccatgaa tggatacctg atgcaggagc aggactgggt cgcgggcgag
     7921 gacctgttcg tggagagttt ctccccggag aacccctatg
//
LOCUS       DATGFG010000795         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1571938, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000795 DATGFG010000000
VERSION     DATGFG010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571938"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1187
                     /locus_tag="VKQ52_14980"
     CDS             171..1187
                     /locus_tag="VKQ52_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88556.1"
                     /translation="MNPSNPAGGCPLAKIDATGKKVISFLKEKVREMYGDERPYRIFH
                     AKTIGLVEAEFIVEDNLSSHLRVGLFASPGRYKAWVRFTNSSATISPDKEKAVRGMAI
                     KIMDVPGTPCPDHTGDPGTQDIVLSNSRSFSPGSGRYALSAVHILLGNLIQKAAGAIR
                     IFSRYFRGSLLFLKYPIVTPNILEEIYYSGTPYSFGEGRRIKWRATPGKTITSVLPEQ
                     PNYDFLRKRLAIDLAENAKDAVEFGLFVQFQQSERTEPIDDSSVVWKTPFHRVATIRI
                     PRQRFTTPERWTLEAGMCFNPGNALVEHAPLGTINRIRVEAYREMAKDRLSHPAKIGR
                     YPYL"
     gene            1198..>2624
                     /locus_tag="VKQ52_14985"
     CDS             1198..>2624
                     /locus_tag="VKQ52_14985"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HLZ88557.1"
                     /translation="MMPVLSLRLLLRLFLPIGFLISWRPAFSQVIDADRCDSIAHLVI
                     TPSAAFMQTELPVSLDSVLRSASRFHQPHDKAVFVANYDPYYYWFRIILNNPRAKSRD
                     LMLLMAPLGLYDGRLFQRVDGKWTQVGQTGLRYRFPDRSYQFTHHVFPFTLPARRVDT
                     LYFSIDASNAYKLFGFALFRPTEFRLFEGKIYFVFGIIVGLLLLFFALNISLFFALKE
                     RLHLWYALYIALLFFVVMKNDQLDQQFLGLDSERAFRLTPYAAAGALAIAVLLHVVQQ
                     FLKKVLIGNRLLYRGSTLLKINIVCSAVAHALVFLTSTDSRIENIVFEWVKISVLVCI
                     CLLIADCLYCVWKGQKSALFLFFGSFVFMVGSIQRLFVGSLSFLFPPTTFHIGIILEI
                     FIISMALIYRYWLEREEQREKEEQVKIKTMNEISTEIHDNVGQTLALANIILQRIDHC
                     QIPIFSEKIGKAESFIGIAIVDLRDL"
BASE COUNT          584 a          709 c          677 g          654 t
ORIGIN      
        1 tccccacgcc ctgcggagaa ccagccggat tgctgccact ttacgacccc gcagaaaacc
       61 atccgcaagc aatatagaga tgttccgccc tttaccacgg tcatcggcgg cgcctatttt
      121 tttctgcccg gcctgcatgc cctctactat ataatcagcc aacaaccgac atgaacccgt
      181 caaatccagc cggcggctgt ccgctggcca aaatagatgc caccggtaag aaagttattt
      241 cttttctaaa agaaaaggtc cgggagatgt atggcgatga gcggccctac cggatcttcc
      301 atgccaagac catcggcctg gtggaggcgg aattcatagt ggaagacaat ctctccagcc
      361 atttgcgagt ggggctgttc gcatctcccg gccgttacaa agcctgggta cgctttacga
      421 acagcagtgc cacgatctcg ccggacaaag agaaggccgt gcgggggatg gctatcaaga
      481 tcatggatgt cccgggaact ccctgcccgg atcataccgg ggaccccggt acacaggata
      541 tcgtcctaag caacagccgc agcttttccc cgggctccgg caggtatgcg ctcagcgccg
      601 tccatatcct ccttggcaat ttgattcaaa aggcagcagg cgcgatccgc attttttccc
      661 ggtatttccg cggatcgctg ttgtttttga aataccctat cgtaacgccg aacatcctcg
      721 aagagatcta ttattcagga acaccctatt cctttggcga gggtcggagg atcaaatggc
      781 gggcaacgcc cggtaaaacc atcaccagtg tgctaccgga gcaacccaat tatgattttt
      841 tgcgcaagcg gctggccatc gatctggcag agaatgcgaa agatgccgtg gagttcggtt
      901 tattcgttca attccagcag agcgaacgca ccgagcccat cgatgatagt tcggtagtct
      961 ggaagacgcc gtttcaccgg gtggcgacca tcaggatacc caggcagcgc ttcactaccc
     1021 cggagagatg gacattggag gccggcatgt gttttaatcc cgggaatgcc cttgtggagc
     1081 acgctcctct tggaaccatc aacaggataa gagttgaggc ctaccgggaa atggcaaaag
     1141 accggctgag ccacccagcg aaaattggcc gatatcccta tttgtaaaat ccaaccaatg
     1201 atgccggtat tgtctctgcg tctgttgctt cgtctttttc tgccgatcgg ttttctgatc
     1261 agctggcgcc cggcattttc ccaggtgatc gacgccgatc gctgcgatag cattgcccat
     1321 ctcgtgatca ccccatccgc ggcctttatg cagacggaac tgcccgtgag cctcgattct
     1381 gtactgcggt cggcatcgcg atttcaccag ccgcatgaca aggccgtgtt cgtagccaat
     1441 tatgacccct attattactg gttcaggatc attctcaaca acccccgggc gaagagccgc
     1501 gacctgatgc tgctgatggc gcccctggga ttgtacgatg ggcgtttatt tcaacgggta
     1561 gacggaaaat ggacgcaggt aggacagacg ggactcagat accggtttcc cgaccggtcg
     1621 tatcaattca cccaccatgt ctttcccttt acgcttccgg cgcgccgtgt ggatacgctc
     1681 tatttcagca tcgatgccag caatgcctat aaattgtttg ggtttgccct cttccggccg
     1741 acggagttta ggctattcga ggggaagatc tattttgtat tcgggatcat cgtgggactc
     1801 ctgcttttgt tctttgccct gaacatctct ctattctttg ccttgaagga acggcttcat
     1861 ctatggtatg ccctgtatat cgccctgctt tttttcgtgg tcatgaaaaa cgatcagctg
     1921 gaccagcagt tccttggcct ggattcggaa agggccttcc ggctaacccc ctacgcggcc
     1981 gcgggggcgc tggccatcgc cgtactattg catgtcgtac agcagtttct gaagaaggtg
     2041 ctcattggca accggttgct ctaccggggc tctaccctgt taaagataaa tatcgtctgc
     2101 agtgcggtgg cgcacgcgct ggtgttcctg acgtccaccg actcgcgaat cgaaaatatc
     2161 gtcttcgagt gggtgaaaat aagcgtcctc gtctgcattt gcctgttgat cgccgattgt
     2221 ctttactgcg tatggaaagg gcagaaaagc gcgctcttcc tttttttcgg ctctttcgtg
     2281 tttatggtcg ggtcgatcca acggcttttt gtcggttcgc tttccttctt atttcctccc
     2341 accacctttc atatcgggat cattttggaa atatttatca tcagcatggc gcttatctac
     2401 cggtattggc tggaaaggga agagcaacgg gaaaaggaag agcaggtaaa gataaaaacg
     2461 atgaatgaga tatcgacgga gatccatgat aatgtcgggc aaacactggc gcttgccaat
     2521 attatcctgc aaaggatcga tcattgccaa atacccatct tttcagaaaa gataggcaag
     2581 gcggagtcat ttattggaat agcgattgtc gacctgcggg atct
//
LOCUS       DATGFG010000796         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_581496, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000796 DATGFG010000000
VERSION     DATGFG010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581496"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1946
                     /locus_tag="VKQ52_14990"
     CDS             102..1946
                     /locus_tag="VKQ52_14990"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88558.1"
                     /translation="MKKLIIYSALVLTMAGVACKKILDKVDFNGVPTATAWGNQSTAL
                     LYLDNLYQLCMPVFYSAASSGTIPTAWHNISDECNGGSTIGVLQGNLTQESETDWFPT
                     ANSYWAYIRKINILFDQIEKYGLTPDFTGPVKAQAYFLRAWSYFQLVKVYGGVPYITK
                     NLDWTTDTLNLPRMSTTACVDSMIADLNHCSILPTTWPSAADQGRITSIAALALKGRI
                     LLYYASPQFNPNNDGSRWQAAYAANEAAYDSAIAAGYALYSNYSRIFLDASTATDKEP
                     ILYRAYNASTSISQNSNNWETAVRPYSMNYSGGGKSYNPTWNLVSAYPMADGHQPRDP
                     NGKYTYDSVRFWQNRDPRFYSSIVYNGAVYGLGSTAGRVQFTYLGTPEEINGPTTTGF
                     YTRRNVDTTLTLVGSQYNKTWWVEMRLAEVMLNLAECANATGNQAEAYTMLEAIRKRA
                     GIPAGTGSYYGLTQGLSQTDLQAAIMNERRIEFAFEDKRYDDLRRTRTFDALNGQYRL
                     AHYLVPRSPWFSSTKVSDTGKVNNIERHIPGSNVTKIRDTVNINGPAFAVFFKDSVAP
                     ITNEQKINFQTSYYFYGIPSSDIGKQPALQQTIGWLYNNIAGTFDPTK"
     gene            1971..>2241
                     /locus_tag="VKQ52_14995"
     CDS             1971..>2241
                     /locus_tag="VKQ52_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88559.1"
                     /translation="MKNRLFLIVTFAAATLGTASRTLAQYPTIPEDVKAQSEALLKET
                     YRRSDSAWQVAWPIIDNDARHGKPYIPWAARPTDLPQSTLLAFPGA"
BASE COUNT          566 a          727 c          514 g          434 t
ORIGIN      
        1 cgcgcagcaa cgagatcacc gactacccga tcctgcgcaa ctggacattc ggattaaatc
       61 ttgctttata aaaacaaccg gaccgtataa acaatataac gatgaaaaag cttatcatat
      121 attctgcact cgttctcacc atggccggcg tcgcctgcaa aaagatcctc gacaaggtgg
      181 atttcaacgg cgtacccacg gcaactgcct ggggtaacca gagcacggcc ctgctctacc
      241 tcgacaatct gtatcagctt tgcatgccgg tcttctatag cgccgccagt tccggcacga
      301 taccaacggc ctggcataac atcagcgacg aatgtaatgg tggttctacc atcggcgtgt
      361 tgcagggcaa tctgacccag gaaagcgaga ccgactggtt ccctaccgcc aactcctact
      421 gggcctatat ccgcaagatc aacatcctgt tcgaccagat cgaaaagtac ggcctgacac
      481 ccgatttcac cgggcccgtc aaggctcagg cctatttcct gcgggcatgg agctattttc
      541 aattggtaaa ggtctatggc ggcgtccctt acataacaaa gaacctggac tggacaacgg
      601 atacgctcaa tctgccccgg atgtcgacca ccgcctgcgt ggatagcatg atcgccgatc
      661 tcaatcactg ctcgatcctt cccaccacct ggcccagcgc cgccgaccag ggacggatca
      721 ccagtatcgc cgcactcgca ctgaagggcc gcatcctgct gtactacgcg agcccgcagt
      781 tcaatccgaa caatgacggt tcccgctggc aggccgccta tgccgccaac gaggctgctt
      841 acgactccgc catcgccgcg ggctatgccc tctattcgaa ttactcacgc atcttcctcg
      901 acgcctccac ggctacggac aaggaaccga tcctgtaccg tgcctacaac gcgagcacct
      961 cgatctctca aaactccaac aactgggaga ccgccgtccg cccctactct atgaactata
     1021 gcggtggtgg taagagttat aaccccacct ggaaccttgt ctccgcctac ccgatggccg
     1081 acggccacca accccgcgac ccgaacggaa aatacaccta cgatagcgtc cgcttctggc
     1141 agaaccgcga cccccgcttc tattcctcca tcgtttacaa cggcgccgtc tatggccttg
     1201 gcagcactgc cggccgggtt caatttacct atctcggtac cccggaagaa ataaacggcc
     1261 ccactaccac cggcttctat acccgcagga acgtcgacac gaccctgacc ctggtgggca
     1321 gccagtacaa taagacctgg tgggtagaga tgcgcctggc cgaagtcatg ctcaacctcg
     1381 ccgaatgtgc caacgcaaca ggtaaccagg ccgaagccta tacgatgctc gaagccatcc
     1441 ggaaaagggc cggcatcccc gccggcaccg gcagctatta tggcctgaca caaggactgt
     1501 cgcagaccga tctgcaggca gccatcatga acgaaagaag gatcgaattc gctttcgaag
     1561 acaaacgcta cgacgatctt cgccgtaccc gtacctttga cgcactaaat ggacaataca
     1621 ggctggcgca ctacctcgta ccccgcagcc catggttctc gtctacaaag gtctccgaca
     1681 ccggtaaagt gaataatata gaacgccata tccccggtag caacgtcacc aagatcaggg
     1741 acaccgtcaa tatcaacgga ccggcttttg ccgtcttctt caaagacagc gttgctccga
     1801 tcaccaatga acaaaagatc aatttccaga cgagctacta tttttatggt attccgtcca
     1861 gcgatatcgg aaaacaaccc gcgctccagc agaccattgg ctggctctat aacaacatcg
     1921 cgggtacatt cgatccgacc aaatgataaa ttgtaaaaaa aatcagcata atgaagaaca
     1981 gacttttttt gattgtgacc tttgccgccg ccaccctggg cacggccagc cggaccctcg
     2041 cacagtatcc tactatcccc gaagacgtaa aagcacaatc agaagccttg ttaaaagaaa
     2101 cctaccggcg gagcgattcc gcctggcagg tggcctggcc cattatcgac aacgacgcaa
     2161 gacacggtaa gccctatatc ccctgggcgg caagacccac cgatctgccc cagtcgaccc
     2221 tcctggcctt tcccggcgca c
//
LOCUS       DATGFG010000797         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_601940, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000797 DATGFG010000000
VERSION     DATGFG010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601940"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2082)
                     /locus_tag="VKQ52_15000"
     CDS             complement(<1..2082)
                     /locus_tag="VKQ52_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLZ88560.1"
                     /translation="MLVLVLLFNFFGYYINRIRSLENKELIAAINCSGHQQALSQRIA
                     KESILLLNNPEPSLAGPLRDTLARIMTVFQQNQDDLQKQAGSTPSPVPQQIFQIKLLL
                     STSRSFFESMNAIGQELIQSDSALIAINKRMYQRDLLYNEQKYNQLMAEINQHYSALV
                     AEKNGEVATIDNGKLISLIVAIAGLILLVLEPAFKKGETNYKELQEARNELLNEKKFL
                     ASILQSQTNYVIRINRAGQFTYANPAFLRTFGYSEQEIQQVLFYTTIFPKDMARCQQV
                     ASECWNNPGKISRLLIRKPIGRTREFLWTEWEFLALTDETGQVNEIQGIGSNVSEKLQ
                     MEQGKEEAIRTLSYAMTYARMGSWKLDFMTSEFSLSRELKALLAMDDEDPDKIPMENF
                     LHLYVVPEDFNQVAEEFARAIQHKETTGYETSFSCRIITKQGWMRYISVKGKVVDEQM
                     EFGIAQDITSQKEAENALLNSEQKFRLLAENSEDIISVHAIDGTIWYLSPSVTTVLGY
                     EVDDIIGRSFEQYVHPDDRHKFLPADQVPQFADKNLAESIIIVRYRILRKDGIYIWLE
                     SIIKPITDEDELVKLICTSRNITDQKIAQEKLKKKDHLLHAVAQATHSLLINTDLNQA
                     IMESIQVLGSTAMVSRVYLFQNHFDKDANQWFTSETHEWTANDADFRLTNPNMRNIPF
                     ATISKILEPLLE"
     gene            complement(2227..>2572)
                     /locus_tag="VKQ52_15005"
     CDS             complement(2227..>2572)
                     /locus_tag="VKQ52_15005"
                     /inference="COORDINATES: protein motif:HMM:NF039584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88561.1"
                     /translation="LTRIRGVHNLTTPEEYLRMIAISRIMLPNVKNIQASWLTVGKQT
                     AQLCLHAGANDFGSIMLEENVVSAAGAPHRFTARGIQAAIREAGFEPQLRNQQYEWRD
                     LPVAMEEQVINY"
BASE COUNT          504 a          607 c          803 g          658 t
ORIGIN      
        1 ctccagcagg ggctcgagga tcttcgatat ggttgcgaaa ggaatattcc gcatgttggg
       61 attggtcagg cggaaatcgg cgtcattggc ggtccattca tgggtctcgg aggtaaacca
      121 ctggtttgcg tccttgtcaa agtggttttg gaaaagatag acccggctta ccatggccgt
      181 gctgccgagg acctggatgg attccatgat ggcctggttc agatcggtat tgatcagcag
      241 ggaatgggtg gcctgtgcga ccgcatgcag gaggtggtcc tttttcttca gtttttcctg
      301 ggcgatcttc tggtcggtga tattacggga ggtacagatc agcttgacca gctcgtcttc
      361 gtcggtgatc ggtttgatga tactctccag ccagatatag atgccatcct tccgcaggat
      421 gcggtagcgg acgatgatga tgctctccgc caggttcttg tccgcgaact gcggcacctg
      481 gtcggcgggg aggaatttat ggcgatcgtc gggatggaca tattgttcga aggagcggcc
      541 gataatgtcg tccacctcgt atcccagaac ggtggtcacg gagggcgaga ggtaccagat
      601 ggtgccgtcg atggcgtgga cgctgatgat atcctcggag ttctcggcca ggaggcggaa
      661 cttctgttcg ctgttgagca gggcgttctc ggcttctttc tgggaagtga tgtcctgtgc
      721 gataccgaat tccatttgtt cgtccaccac tttgcctttt acggagatat accgcatcca
      781 gccctgtttg gtgatgatcc ggcaggagaa ggaggtctcg tagccggtag tttctttatg
      841 ctggatggcc cgggcgaatt cctccgccac ctggttgaaa tcttcgggaa cgacatagag
      901 gtggaggaag ttctccatgg ggatcttgtc cggatcttcg tcgtccatgg ccagcagggc
      961 ctttagctcc ctgctgagcg aaaattcact ggtcatgaaa tccagtttcc aggagcccat
     1021 ccgggcgtag gtcatggcgt aggacagggt gcggatcgcc tcttcttttc cctgttccat
     1081 ctgcagcttt tccgagacat tggagccgat gccctggatc tcgttcacct ggccggtctc
     1141 gtcggtgagg gcgaggaact cccattcggt ccacaaaaac tcgcgcgtgc gcccgatggg
     1201 tttacggatc agcagccggg agatctttcc ggggttattc cagcattcgc tggcgacctg
     1261 ctgacagcgg gccatgtcct tcgggaagat ggtagtataa aaaagcacct gctggatctc
     1321 ctgttcggaa tacccgaagg tcctcagaaa ggcggggttg gcgtacgtga actgaccggc
     1381 ccggttgatg cggatgacat agttggtctg cgactgcagg atggaggcaa ggaatttctt
     1441 ttcattcagc agttcgttcc gcgcctcctg gagttctttg tagttggtct cccctttctt
     1501 gaaggcgggc tcgagcacca gcaggatgag gccggcgatg gcgacgatca gggagatcag
     1561 ttttccgttg tcgatggtgg ctacttcgcc gttcttttcc gcgaccagcg ccgaatagtg
     1621 ctggttgatc tccgccatca gctggttgta cttttgttcg ttgtacaaca ggtcacgctg
     1681 gtacattctt ttatttatcg cgatcaatgc ggaatccgac tggatcagct cctggccgat
     1741 ggcgttcatg gactcgaaga aggaccggga ggtggagagc agcaatttga tctggaagat
     1801 ctgttgcggc acgggggagg gggtggagcc cgcctgtttt tgcaggtcgt cctggttttg
     1861 ctggaagacg gtcattatcc gggcgagcgt gtcccggagg ggtccggcga gggagggctc
     1921 ggggttgttc agcaagagga tagactcttt tgcaatgcgc tggctgaggg cttgctggtg
     1981 tcctgagcaa ttgatcgcgg cgatcagttc cttattctcg agtgacctga tccggttaat
     2041 ataatacccg aaaaagttga acaggagaac caaaaccaac aagacaaccg atatacgtac
     2101 gagtttgcga aaagggcttt tcatgaatgt tatgcaaagg gtcttcgctt ataggaacgt
     2161 aaaatcagga ttttaagttt ccggagggcc ttaaaaatat aggggcaaac acttacattt
     2221 tgaaaattaa taatttataa cttgttcttc cattgcgacg ggcagatcgc gccactcata
     2281 ctgctggttg cgtaactggg gttcgaagcc ggcttcccgg atggcggcct ggatgccgcg
     2341 ggcggtaaac cgatgggggg cgccggcggc gctgaccaca ttttcttcga gcatgatgct
     2401 gccgaagtca ttggctccgg cgtggaggca taattgcgcg gtctgtttgc cgacggtgag
     2461 ccaggaggcc tggatgtttt tgacattcgg gagcatgatc cggctgatgg cgatcatccg
     2521 gagatattct tcgggtgtcg tcaggttatg tacgccgcgg atacgggtga gg
//
LOCUS       DATGFG010000798         4371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_91814, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000798 DATGFG010000000
VERSION     DATGFG010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91814"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VKQ52_15010"
     CDS             complement(<1..1157)
                     /locus_tag="VKQ52_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ88562.1"
                     /translation="MKLFTKYYRINLPVMMVIFLLSAGLNYLLIDQVLVREVDISLAG
                     AEKGIRTYYAAHHQFPQQYPLDDSRLSYELTDKPMATSLYSPITLYSNLESKMHNFRK
                     VVFTISDNGKIYKVSFSRPLQGIHHLSRAIIFVSLLTVLTTLLAYFITNRMLLRRLWR
                     PFYDSLHAMRNLQLGNAGNLHFPVTSTEEFAYMNESLGMAARKAGEDYLLLKEFTENA
                     SHELQTPLSAIRSKLEILIQDEDLSSKQSEIVSSLFASLKKLSRLNQSLLLLTKISNQ
                     QFHQLEQIDLREKMEDKLVQFRELWSANRIAASWQLEEASIEASPDLIDILLNNILSN
                     AGNHNITNGLIHILLEPNKLTVSNTSPSPALDPKRVFTRFYKGELNTGNNGL"
     gene            complement(1154..1846)
                     /locus_tag="VKQ52_15015"
     CDS             complement(1154..1846)
                     /locus_tag="VKQ52_15015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88563.1"
                     /translation="MKVLIIEDQQELSDSILAYLARENFTCEVAYDFETAREKATNED
                     YSCIILDISLPLGNGLNILQELKARNKAEGVLIISARNSLDDKILGLNLGADDYLTKP
                     FHLPELAARVSAIIRRMSLDDKHRIVLNTLSLDLRDRIVRGAKGIIDLTRKEYDLLVY
                     FMGNINKVVTKEAIVEHLWGEQIDMADNYDFIYAHIKNLRKKLMQAGCPDHIKAVYGM
                     GYKFTAKEEKRK"
     gene            complement(1901..2488)
                     /locus_tag="VKQ52_15020"
     CDS             complement(1901..2488)
                     /locus_tag="VKQ52_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLZ88564.1"
                     /translation="MVTAILLAAGSSQRMGAPNKLLLPWQGKPIVAATAEQILAAGID
                     ELIVVTGHEATETAAALTHLPVHIVHNPAFVTGMTGSIQTGVRLARGDGYMICLADMV
                     LITAREYQELAAAFDLRYRRDPHCILLPEYQGQKGNPVIFSAAWREAILGHPEEEGCK
                     SLVRRHPDHHLYISMTTNHILQDIDFPEDYRALTT"
     gene            2596..3327
                     /locus_tag="VKQ52_15025"
     CDS             2596..3327
                     /locus_tag="VKQ52_15025"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLZ88565.1"
                     /translation="MKKPLLFACLLCIGLFAARSLPAQQVAPGSPQPALGSPQPALSA
                     QAPFWSEIADFKRRDSLHHPPSNAILFVGSSSFRKWTNVQADFPGYTIINRGFGGSTL
                     DDVIHYAGDIIYPYKPKQVVIYCGDNDLASDRKVSGKKVYKKFGHLYDLIRKHLGNDV
                     DILYVSIKPSPSREKLMPEMERANDMIRNFMAQRSHASFVDVYHLMLNAQGHPMDDLF
                     IGDKLHMSEKGYKLWQQAILPYLDK"
     gene            3566..>4371
                     /locus_tag="VKQ52_15030"
     CDS             3566..>4371
                     /locus_tag="VKQ52_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88566.1"
                     /translation="MSKDTMFPRKKILFIIGSPNQASQMHQIASHLPDYDCFFSQLYS
                     KHPVVKAAVRTGFLDTTILAGEFKRKGDAYLERHGLPNDYAQDVYHNNYDLTVLCSDL
                     LVTKELRRGKTIWVQEGMTDPVTAWGKLTRRLGLPAYWARNTAFNGCSDICDIYCAAS
                     PGYKDQFSRLGTDADKIIVTGIPNYDNAAALLNNDFPRKGYVMVATSDVRETFNKDDR
                     PAFIRNCVRIAAGRPLLFKLHPNEKRERAIAEIKEWAPSAAIYTEGNTEQ"
BASE COUNT          925 a         1005 c         1340 g         1101 t
ORIGIN      
        1 agcccgttat tgccggtgtt cagttctcct ttatagaaac gggtaaagac gcgttttgga
       61 tccagggccg gggacggcga ggtattgctg accgtgagtt tgttgggttc cagcaggata
      121 tggatcaaac cgttcgtaat attgtgattg cccgcattgc tcaggatatt gttcagcagg
      181 atatcgataa ggtcggggct ggcttcgatg cttgcctctt ccagttgcca ggaggcggcg
      241 atcctattgg ccgaccagag ttcccgaaac tgtaccagtt tgtcctccat tttctcccgg
      301 agatcgatct gctccaattg gtggaactgc tgattgctga tcttggtcag caggagaagg
      361 gattgattga gacgggaaag ttttttcagg gaggcaaaga gactgctgac gatctcgctt
      421 tgtttcgagg agaggtcttc gtcctggatg aggatctcga gtttggaccg gatggccgac
      481 aatggggtct gcagttcatg agaggcgttc tcggtaaact ctttcagcag caggtagtcc
      541 tcaccggcct tacgggctgc cattcccagg ctctcgttca tataggcgaa ctcttcggtc
      601 gaggttacgg gaaaatgtaa attaccggcg ttgcccaatt gcaggttgcg catggcgtgg
      661 agggaatcat aaaagggccg ccacagacgg cgtaagagca tgcggttggt gatgaagtag
      721 gcgagtaagg tggtaaggac cgtcagcagc gatacgaaga tgatggccct ggacaggtga
      781 tggatgcctt gcagcggtct tgaaaaacta accttgtaga tctttccgtt gtcgctgatg
      841 gtaaagacca ccttccggaa attatgcatt ttactttcca ggttgctgta cagcgtgatg
      901 ggggagtaaa ggctcgtggc catgggctta tcggtgagct catagctgag ccggctgtca
      961 tcgagcgggt attgctgggg gaattgatgg tgggcggcgt aataggtcct gattcctttt
     1021 tctgcgcccg ccagggaaat atccacttcc cggacgagga cctggtcgat cagcaggtag
     1081 ttcaatcccg cggagagcag gaagatgacc atcatgaccg gcaggttgat ccgatagtat
     1141 ttggtgaaca gcttcatttc cttttttctt cctttgcggt gaatttatat cccatgccat
     1201 agacggcctt tatatgatcg gggcagccgg cctgcatcag tttcttccgg aggtttttga
     1261 tatgggcgta gataaaatcg taattatcgg ccatgtcgat ctgttcgccc cagaggtgct
     1321 ctacgatggc ttccttggtt acgaccttgt tgatattccc catgaaatag acgagcaggt
     1381 cgtattcttt gcgggtgaga tcgatgatcc ctttggcgcc cctgacgatc ctgtcccgca
     1441 ggtcgaggct gagggtattg agaacgatcc ggtgtttatc gtcgagggac atcctccgga
     1501 tgatcgcgct tacccgggca gccagttcgg ggaggtgaaa gggttttgta aggtagtcat
     1561 cggctcccag gttcaggccc aggatcttgt cgtccaggga gttccgggcg gatatgatga
     1621 gtacgccctc cgctttgtta cgggctttta gttcctgcag gatgttcaat ccgttgccca
     1681 ggggaaggct tatgtccagg atgatgcagg agtaatcttc gttggtggcc ttttcccttg
     1741 ccgtttcgaa gtcataggcg acctcacagg taaaattctc ccgggcgaga taggcgagga
     1801 tgctgtccga taattcctgt tggtcttcga tgatcagaac cttcatttgc ggtggattgt
     1861 ggcaaaagtc tggaaggatt ctgcaataat tctgaagggg ttaggtggtc agggcccggt
     1921 aatcttccgg gaagtcgata tcctgcagga tgtgattggt tgtcatggag atataaagat
     1981 ggtgatccgg gtgccggcgg acgaggcttt tacagccttc ttcttcgggg tgtccgagaa
     2041 tggcttcccg ccaggcggcg gagaagatga ccgggttgcc tttttgtccc tggtattcgg
     2101 ggaggaggat gcaatggggg tcccggcggt accggaggtc gaaggctgcc gccagttcct
     2161 ggtattcccg ggcggtgatc aggaccatgt cggccaggca gatcatgtag ccatcgccgc
     2221 gggcgaggcg gacgccggtt tggatggacc cggtcatccc ggttacgaaa gccgggttat
     2281 ggacgatgtg gacggggagg tgggtgaggg cggcggctgt ctccgttgct tcgtggccgg
     2341 tgacgacgat gagttcgtcg atgcctgctg ccaggatctg ttccgcggtg gcggccacga
     2401 tgggtttacc ctgccaggga aggagcagtt tattgggggc gcccatgcgt tgggaactgc
     2461 cggctgccag gaggatggcg gtgaccatgg ggtaaagtta gccgaataaa ggaaaaaatt
     2521 atgcaattcg ggtgccagtg gggaaatcct atttccggct ttcgtagctt tggcgacacc
     2581 atggaaaagc cacatatgaa aaaacccttg ttgtttgcct gcctcttgtg tatcggatta
     2641 ttcgccgcgc gaagtctgcc ggctcagcag gtggcgccgg ggtcgccgca gccggcactc
     2701 gggtcgccgc agcccgcgct ttccgcacag gctcctttct ggagtgagat cgccgatttc
     2761 aagcggcggg acagtcttca tcatccgccc tccaatgcca tcctttttgt gggaagttct
     2821 tcctttcgca aatggaccaa tgtgcaggcc gatttccccg ggtatacgat catcaatcgt
     2881 ggttttggcg gttccacgct cgacgatgtg atccattatg caggagatat catctatcct
     2941 tacaagccga agcaagtggt tatttactgc ggggataacg atctcgcgtc cgacaggaag
     3001 gtctcgggga agaaagtgta taagaagttc gggcatctgt atgatctgat cagaaaacat
     3061 ctgggcaacg atgtcgatat cctgtatgtc tcgatcaagc cgagtccgag cagggagaag
     3121 ctgatgccgg agatggagcg ggccaatgac atgatccgga attttatggc gcagcgtagt
     3181 catgcgtcgt ttgtggatgt atatcacctg atgctgaacg cgcagggaca tccgatggat
     3241 gatcttttca tcggggataa gctgcatatg agtgagaagg gatataagct ctggcagcag
     3301 gcgatactac cttatctgga taagtagtgt aattactaca cggccgcgct cgttctgtcc
     3361 cgggatggga atctaatgtc gggccttcgg ccgccggacc cgtggtccgg gcctgctatt
     3421 tccttatgat attaaggaat tgtgattttc gcgtccggct gaatcttgta cctaagatag
     3481 gaagaaattt gcggattaat taggcaagct tgttaacttt attaccgaaa ggttgttatt
     3541 cgctggtaaa ccaagctgct aactcatgtc gaaggatacc atgtttccac ggaaaaagat
     3601 acttttcatc ataggatcac ccaaccaggc cagtcagatg caccagatag cgtctcattt
     3661 gcctgactat gattgtttct ttagtcagct atacagcaag cacccggtgg tgaaggccgc
     3721 ggtgcgtacc gggtttctcg atacgaccat cctggcgggg gagttcaagc ggaaaggcga
     3781 tgcctatctt gaaaggcacg ggttgcccaa tgactatgcg caggacgtat accataataa
     3841 ttatgacctt acggtattat gctccgatct gctggtgaca aaggaattgc ggaggggcaa
     3901 gacgatctgg gtgcaggagg ggatgaccga tcccgttacc gcctggggaa aattgacccg
     3961 gcggctgggg ttgccggcgt actgggcacg gaatacggcc ttcaacggat gcagcgatat
     4021 ttgtgatatc tattgtgcgg cctctcccgg ttacaaggat cagttcagcc ggctgggcac
     4081 cgatgcggac aagatcatcg ttacgggtat tcccaattac gataatgcgg ccgcgctgtt
     4141 gaacaatgat ttcccgcgca agggatatgt gatggtggct acatcggatg tgcgggagac
     4201 ctttaacaag gatgatcggc cggcttttat ccggaattgt gtccggatcg cggcggggcg
     4261 gcccttgctt tttaagcttc atcccaatga gaagagggag cgggccattg ccgagataaa
     4321 ggagtgggcg ccttccgccg ccatctatac ggagggcaat acggagcaga t
//
LOCUS       DATGFG010000799         3705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_817198, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000799 DATGFG010000000
VERSION     DATGFG010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817198"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2084)
                     /locus_tag="VKQ52_15035"
     CDS             complement(<1..2084)
                     /locus_tag="VKQ52_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88567.1"
                     /translation="MKITGSTVFDILLRWRVYFQGNNALRDYHGGEPFRSELFSSEQM
                     DRHGIVVAQSHKLMKRHSSDQLLGRLDGNEKTLLEVRSLLVENIRAGKPITPGAEWLL
                     DNFYLIEEQVIIARKHLPKGYSEALPYLSQGMSHGMPRVYDIVLEIISHSDGRVDVKG
                     LSSFIAAYQTYTPLTLGELWAIPIMLRLSVIENLRRVAGKIALDMIDRNMADYWADKM
                     METAKEEPGNLILTIADMVRSKPALVSPFVAAFTKKLQGKGPALALPLNWMEQQLSAL
                     KLNSADLVQQENQKQAADQVSVRNSIGTLRAIGATDWREFVESSSSVEQILRQDGAGI
                     YPLMDFATRDSYRHVVESIAKASPLSETEVAAQVLALAGRPLPGGEGILPDAHSPAGK
                     HAHVGYYLVDKGRIQTEKAACMRYTLRQKLARIAGRVPVLLYLSSVTLLTMLTAAGVL
                     YPLYRYGPHHWLLLSVMALLSLSVSAQLAIPLVNWLSTLWIKPMLLPRMDFSTGIPAR
                     YRTLVVVPSMLSGTAYIAEMIEALEIRFLANKENNLYFGLLTDFTDAGSQVMPDDDAL
                     LDFASRRIQALNKKYSPEGQMGSGQGGSCQDIFFLFHRPRAWNRKERKWMGYERKRGK
                     LFALNCLLRGRSPHEFSRITGNHRLLSGIKYIITLDSDTLLPRDSARAFIATMAHPLN
                     HALYDPKKRRVT"
     gene            complement(2101..3171)
                     /locus_tag="VKQ52_15040"
     CDS             complement(2101..3171)
                     /locus_tag="VKQ52_15040"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HLZ88568.1"
                     /translation="MPKITFNNSDRSFFQSVKHSVDAYFEARHLRKTGNWRLYLKAII
                     LIPLAMSIYCFLLWGRYSWPPGILLSCLLGLTLVCIAFNVMHDACHGSYSRKKWVNEC
                     LGLTMNALGSNAFFWKIKHNIIHHTYTNIDGADADIANEPLLRQCSTQKWRPIHRYQF
                     LYMFVLYSISTLAWMWGTDFVKYFSRKIHATPIRKIEFREHLIFWLSKLFYIFFYVAV
                     PIYFVGWQPWLIGFSIIHGTMGLTLSIVFQLAHVVEKTAFESAHYEPKVIAAEWAIHE
                     VRTTADFAPRNHVISWLAGGLNFQIEHHLFPQISHIHYQAISKIIRQQCELFGLPYNY
                     YPSMRLAIHSHIQLMKELGRKR"
     gene            complement(3304..3522)
                     /locus_tag="VKQ52_15045"
     CDS             complement(3304..3522)
                     /locus_tag="VKQ52_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88569.1"
                     /translation="MDNLIGNSTGQDTGVEKSRAHIIVEIIEYMPNSIVIKTIIRKST
                     GNISVMSFDSGEGLTEETSEFHKAQWPV"
BASE COUNT          856 a          917 c          987 g          945 t
ORIGIN      
        1 tccgtcaccc tccgtttttt cgggtcataa agggcatggt tgagggggtg ggccatggtc
       61 gctataaagg ctcttgcgga atccctgggg agcagggtat ctgaatccag ggtgatgata
      121 tatttgatcc cggacaggag cctgtgattg cctgtaatcc tggagaattc atgagggctg
      181 cgtccccgca agaggcaatt cagcgcgaac aattttcctc tttttcgttc atagcccatc
      241 cattttcttt cctttctatt ccaggccctt gggcggtgaa agagaaagaa gatatcctgg
      301 caggagccac cctgccctga gcccatctgg ccttcggggc tatacttttt attcagggct
      361 tgaatcctgc ggctggcaaa gtcgagtaat gcatcgtcat ccggcatgac ctgggaaccg
      421 gcgtccgtga aatccgtcaa aagcccaaaa tacaagttgt tctctttatt tgcgaggaaa
      481 cggatttcca gggcctctat catttctgct atgtaggctg tgccggaaag catcgacggg
      541 acgaccacca gggtgcggta ccgggcaggt atgccagtgg agaaatccat tcggggcagg
      601 agcatgggtt ttatccaaag ggtagacaac cagttcacga gaggtatggc caattgagcc
      661 gagactgaga gggacaataa ggccattacc gacagcaata accagtgatg cggaccatac
      721 ctgtagagcg ggtataacac tcctgccgca gtcagcatgg ttagtagcgt cacagatgac
      781 aggtacagca atacgggtac ccggccggca atgcgggcaa gtttctgacg caaagtatag
      841 cgcatgcatg cagccttctc cgtttgtatc cttcccttat ctaccaggta gtagccgaca
      901 tgcgcatgct tgccagccgg cgaatgtgca tctggaagta tgccttcacc gccagggagg
      961 ggtcttcccg ccagcgccag cacttgcgct gccacttccg tttcggacag cggggaagct
     1021 ttggctatgg attccaccac atggcggtag ctatcccggg tggcaaaatc catcaggggg
     1081 tagataccgg cgccatcctg tctcaatatc tgctccacgc tgctgctcga ttcgacgaat
     1141 tcgcgccagt cagtcgcgcc aatcgcccgc agggtgccga tgctattacg tacggagacc
     1201 tgatcggcgg cctgtttctg gttctcctgc tgaacgaggt cagcgctatt cagcttcaat
     1261 gccgacaatt gttgttccat ccaattgagg ggaagtgcca gggcgggtcc tttaccctgc
     1321 agcttttttg taaacgctgc aacgaaggga ctgaccaggg cgggttttga gcggaccata
     1381 tcggcaatcg tcagaatcag attccctggt tcctctttcg ccgtttccat cattttatct
     1441 gcccagtaat ccgccatgtt gcgatcgatc atgtccaggg cgatcttccc ggccacccgg
     1501 cggaggttct ctatgacgga gagccggagc ataatgggta tggcccataa ttccccaagt
     1561 gtgagcgggg tgtaagtctg ataggctgcg atgaagctgc tgagcccttt cacgtccacg
     1621 cggccatcgc tatgggaaat gatctccagg acaatatcat agacacgggg catgccgtga
     1681 gacatacctt gggacagata gggcaatgct tcgctatatc ctttgggaag atgttttctg
     1741 gcgatgatga cctgttcttc tatcaggtaa aaattgtcga gcagccactc ggcgccggga
     1801 gtgatgggtt tgccggcgcg tatattttca accagcaggc tgcgcacttc cagcagggtc
     1861 ttttcattgc cgtccagtct tcccaggagc tgatctgaag aatggcgctt catcagctta
     1921 tgggactggg ccactacgat gccatggcgg tccatctgtt ccgagctgaa aagttccgaa
     1981 cggaatggct cgccgccatg ataatcccgg agggcattgt tgccctggaa atagacacgc
     2041 caacgtagca ggatgtcgaa aaccgtactg cctgtaatct tcataaagat cttcttgtga
     2101 ttaacgcttc cttcccaatt ccttcattag ttggatatgc gagtgaatag ccagccgcat
     2161 ggacgggtaa taattgtagg ggaggccgaa cagttcgcac tgctgcctga taatcttgct
     2221 gatcgcctga taatgaatat ggctgatttg gggaaacaag tggtgttcga tctggaagtt
     2281 gaggccaccg gccagccagg agatcacatg attgcggggc gcgaagtcag ctgtagtcct
     2341 tacttcatgg atggcccatt cggcggctat cactttgggc tcgtagtgtg ccgattcgaa
     2401 ggccgttttc tccactacgt gggccaattg aaatacgatg gagagggtca gccccatcgt
     2461 accatgaatg atagaaaagc cgattaacca gggctgccag ccgacgaaat agatcgggac
     2521 ggccacgtag aagaagatat agaagagctt gctgagccag aatatcaggt gctcccggaa
     2581 ttctattttt ctaataggcg tagcatggat cttcctggaa aaatatttga cgaaatcggt
     2641 gccccacatc caggccagcg tgctaatgga gtaaagaacg aacatataaa ggaactggta
     2701 cctgtgaatg ggcctccatt tctgggttga gcattgtctc agcaatggct cgttggcgat
     2761 atccgcgtcg gctccgtcga tattggtata ggtgtggtgt atgatattat gcttgatctt
     2821 ccagaagaag gcgttgctgc ccagggcgtt catggtgagc cccaagcatt cattgaccca
     2881 ttttttccgg gaatagctgc cgtgacaggc atcatgcatg acattgaaag caatgcatac
     2941 cagggtaaga ccgagaaggc aggaaagcaa tatgccaggc ggccaactgt agcgccccca
     3001 caggagaaag caatagatgc tcatagccag cgggatcaat atgatggctt tcaggtataa
     3061 cctccagtta cctgtcttgc ggagatgccg ggcttcgaag taggcatcca ctgaatgttt
     3121 gaccgattga aagaacgagc gatcgctatt attgaatgtt attttgggca tgtcttatgc
     3181 ccctaaaggt ccgtttttta gcgcccggtc cgcttatatg attctgtcga attgttgtat
     3241 tattcacaca ttataccctt caggtcttat tcatatccac ttttgatgac ggtcgagatc
     3301 attttaaacc ggccattggg ctttgtgaaa ttcggaggtc tcctccgtca gcccctctcc
     3361 actgtcgaaa gacataacac tgatattccc ggtcgatttc ctgatgatcg tcttgatgac
     3421 gatcgaattg ggcatgtatt cgatgatttc gacgatgatg tgtgccctgg atttttccac
     3481 gccagtgtcc tgtcctgtgc tattgccaat tagattgtcc atgatactga ttgtaaaaac
     3541 tatcgcctgc caaaggaaac ccggcggtag ttttgtttga aagggtgcct acagaccgtc
     3601 aatgatctta ttcagatcgt ccttcgtttt cccaagtttg agctgaagct tgttcatcat
     3661 ctcgtctttc ttaccggata caaactgcag atcactatcc gtcaa
//
LOCUS       DATGFG010000800         4832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_422849, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000800 DATGFG010000000
VERSION     DATGFG010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422849"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VKQ52_15050"
     CDS             complement(<1..203)
                     /locus_tag="VKQ52_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88570.1"
                     /translation="MKLTGLILLLFIAYSPLFAQYYFTGEVKDLHGDKLQRVAILVQS
                     TGLVYQTGRYGDFEITSAKADDSL"
     gene            complement(274..960)
                     /locus_tag="VKQ52_15055"
     CDS             complement(274..960)
                     /locus_tag="VKQ52_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88571.1"
                     /translation="MRIDCIIIEDEPLAQERIRGYLQRLPFMRLLGVFDNGIEALAFL
                     QNGEPDVIFLDINMGDFSGIQLLESMKLSSQVIISTAYHEYALKGFELRVADYLLKPY
                     TFERFVQAIDRVRSLRSRPAGVTEKRFIFVKTEHRLEKLLLGDLLYIEGMRDYRRIFA
                     RDKQILTLQTFGELEQNIPPAIACRVHKSYMVALDKIDVVERDVIRVGEVEIPLSETY
                     RKPFLQWLMG"
     gene            complement(950..1981)
                     /locus_tag="VKQ52_15060"
     CDS             complement(950..1981)
                     /locus_tag="VKQ52_15060"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ88572.1"
                     /translation="MRRIAIIGFHVCYWMMYGLLIFLFAILSAHTIHFPLKRLLIPGA
                     FFTLPAISSFYIFYFVLFPRFLQRKRLLLLSVIAIGVSLFCAVETLTAMSLLLGSRVQ
                     VSFTMDSAGEILAMAFIAFVNGVLALVIRGFITSYGDIRVKEELRRKNTEMELTLLRS
                     QLHPHFLFNTLNNIDVLIEKDAARASGYLQQLSGLLRYLLYEVRAERIPIRDELQTIE
                     RYIHLQKIRTTHPESIHYTVEGETDRWMIEPMLFLPIIENAFKHAEKRAENAIRIRFQ
                     LDRNRIRFDCENRYQRKPDKAEDAAGGLGNSLIRKRLMLLYPGRHTLQVEAGPETYKT
                     TLILTGHAN"
     gene            complement(1984..2745)
                     /locus_tag="VKQ52_15065"
     CDS             complement(1984..2745)
                     /locus_tag="VKQ52_15065"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88573.1"
                     /translation="MSTFAYSFSSEWLKKKRTVASLLTVIGSLLIPMIVLISRLDGGG
                     LAAANRQPRLWEGLYIRNWQLMGLLLLPMGVVLATSLITQLEFRNNTWKQWCTTPQSL
                     TTIFLVKLSVILVMLAEFFLLFTLGIWLTGVLPALFFRDVPYPLESFPWKAISVSSAR
                     FFLDILPVVALQYLLSLRFRNFLVPLGAGLGLYVASMIAVHWRHGYWIPYTYSAYNFM
                     GKAPEKWHTHSWAAGYTLVFIGLAYILYITRKEKG"
     gene            complement(2742..3467)
                     /locus_tag="VKQ52_15070"
     CDS             complement(2742..3467)
                     /locus_tag="VKQ52_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ88574.1"
                     /translation="MSNYCLETAGLCHRFSAGVSVLNKIDLRVPEGSIYGFLGPNGAG
                     KTTTLRLVLGLLKKQQGEISVFGKPFAPHRISILKKLGSMIESPSVYAQLTAAENLRV
                     LQKIYRCPEERIPYVLQLTGLGDTGGKKAGQFSLGMKQRLGIAIALLHDPALLILDEP
                     TNGLDPEGIIGMRELFRTLNGQGVTILVSSHLLAEMEKLITHAGIIHKGQLLFQGTLE
                     QLREGRKGDDLETIFIQLTKQRA"
     gene            complement(3547..4443)
                     /locus_tag="VKQ52_15075"
     CDS             complement(3547..4443)
                     /locus_tag="VKQ52_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLZ88575.1"
                     /translation="MKNTSHYLAAISAFTIWGFFSLVLRPLAVYPSLDLLFYRVFLSA
                     LLMLLTGVLLRRKVWVDNRRLFVSSPDAVKKGVLFQLLGGGLFLTANWFFFIYVTNQV
                     SIKAAAFAYLVCPVLTTVLAWLILKEHLTKGQWVAVLLSAAGCGILAFHHLGDLVYSL
                     VIAVSYALYLVSQRKDYGVDRFLLLSGQILFSALLLLPFYPAWHGPVPQESRFYILMA
                     VIAVLLTIFPLWLNLYALKQLKSSTVGILLYINPVLGFMLALTVYGEKVDALQLSAYG
                     VIVVAVALFNWFNPAIRPQSQK"
     gene            4578..>4832
                     /locus_tag="VKQ52_15080"
     CDS             4578..>4832
                     /locus_tag="VKQ52_15080"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HLZ88576.1"
                     /translation="MPNLYPGIKPTIGSGRSYVFLSIVLFLPFLVLAQPVITSFTPQA
                     GAVGSTVTINGANFGTTPTANIVFFGSVQATVTAATATSLT"
BASE COUNT         1156 a         1341 c         1348 g          987 t
ORIGIN      
        1 agggaatcgt ccgccttggc ggaggtgatc tcgaaatcgc catatcggcc ggtctggtag
       61 accagaccgg tggactggac gagaatggcg acacgttgca gtttgtcacc gtgcaggtcc
      121 tttacttccc ctgtgaaata atattgggca aagagagggg agtacgcaat aaaaagcagc
      181 agtattagac cagtcagttt cacaaactcg gtttgccgcg taaaactacg aaaattttcg
      241 gaaaaacggc acggtcaccc gccggagtcc gccctacccc atcagccatt gcaggaacgg
      301 ttttcggtag gtctcggaca agggtatctc cacctcccct accctgatca catcgcgttc
      361 aacgacgtcg atcttgtcaa gggcgaccat ataggattta tgtacccggc acgcaatagc
      421 gggcgggatg ttttgctcca gttcaccaaa ggtctgaagc gtaaggatct gtttgtcccg
      481 cgcaaagatg cggcgatagt cacgcatccc ttcgatatac agcaagtctc cgagcagcag
      541 tttctccagc cggtgctccg tctttacgaa gatgaaccgc ttttccgtta cgcccgccgg
      601 ccggctcctc agcgagcgta cccggtcgat agcctgtaca aatcgctcga aggtgtaggg
      661 cttcaacagg tagtccgcca cccgcagctc gaaccctttc agcgcatatt catgataggc
      721 agtactgatg atcacctgac tgctcagctt catcgactcg agcagttgta tccccgagaa
      781 gtcgcccata ttgatgtcga gaaagatgac atcgggttcg ccgttttgca gaaaggccag
      841 ggcctcgatg ccgttgtcaa agacaccgag cagccgcatg aacggcagcc gttgcagata
      901 tccgcggatg cgttcctgcg ccaggggctc gtcctcgatg atgatacagt caattcgcat
      961 ggccggtgag gatgagggtg gtcttatagg tttcggggcc ggcctccacc tggagggtat
     1021 gccgtccggg atacaggagc atcagtcttt tccggatgag gctgttaccc aggccgccgg
     1081 cggcgtcttc tgccttatcc ggtttccgct ggtaccggtt ctcacagtcg aaccggatcc
     1141 tgttcctgtc cagctgaaag cggatacgga tggcattctc cgcgcgcttt tcggcatgtt
     1201 taaaagcatt ctcgatgatc ggaaggaaga gcatgggttc gatcatccac cggtcggtct
     1261 cgccttctac cgtatagtgg atcgattcgg gatgcgtcgt ccggatcttt tgcagatgga
     1321 tatacctttc gatcgtctgt aattcgtccc tgatcgggat ccgttccgcc cttacttcat
     1381 agaggaggta tcggagcagg cccgacaact gctggagata gccggaggcg cgggcggcat
     1441 ccttctcgat cagcacatcg atgttgttga gggtattgaa cagaaaatga ggatgtaact
     1501 ggctgcgcaa caaggtcagt tccatctccg tatttttccg ccggagttcc tccttcaccc
     1561 ggatatcgcc atacgaagtg ataaatcccc tgatcaccag cgccaggacc ccgttgacaa
     1621 aggcgatgaa cgccatcgcc aggatctcgc cggcggagtc catggtaaag ctgacctgta
     1681 cccgcgatcc aaggaggagg ctcatcgccg tcagggtttc caccgcgcaa aagagggaca
     1741 ccccgatggc gataacgctc agcagcagca accgcttccg ctggaggaac cgcgggaaca
     1801 gcacgaagta aaagatatag aaggaggaaa tagccggaag ggtaaagaaa gcgccgggga
     1861 tcagcaatcg ctttaaaggg aaatggatgg tatgcgcact caggatcgcg aaaaggaaga
     1921 tcagcaaccc atacatcatc cagtaacaga catgaaaccc aatgatggcg atccttctca
     1981 tgattatcct ttttccttgc gcgtaatata aagaatatat gccaatccga tgaataccag
     2041 ggtataaccc gctgcccagg aatgcgtatg ccatttctcc ggggccttgc ccataaaatt
     2101 ataggcgcta taggtatagg gtatccaata accgtggcgc cagtggacgg cgatcatcga
     2161 ggccacatag agtccaagcc cggcccccag cgggaccagg aaattcctga atcggaggct
     2221 aagcaggtat tgcagggcga cgacaggcag gatgtcgaga aagaaccgtg cgctgctgac
     2281 ggaaatggcc ttccacggaa aggattcgag gggataggga acatcccgga agaaaagcgc
     2341 gggaagaacg cccgtcagcc agatgccaag ggtgaacaac aggaagaact ccgccagcat
     2401 caccaggatc accgacagtt taaccaggaa gatcgtcgtc agcgattgcg gggtcgtaca
     2461 ccactgcttc caggtattgt tccggaattc cagttgagtg ataagactgg tcgcaagcac
     2521 cacgcccatc ggcagcagga gcaggcccat caactgccag ttgcggatgt acaggccttc
     2581 ccagagccgg ggttgccggt tggcggccgc aagcccgccg ccatcgagcc gcgaaataag
     2641 gacaatcatc gggatcagga ggctgccgat caccgtgagc agggaggcga ccgtccgttt
     2701 cttcttcagc cattcgctgc tgaaactata ggcgaaggta ctcatgcgcg ttgttttgtc
     2761 agttggataa aaatggtttc caggtcatcc cctttccggc cctcgcgcaa ttgttccagt
     2821 gtcccctgga acaacagctg tcccttgtgg atgatcccgg catgggtgat cagcttctcc
     2881 atctctgcca gcaggtgact ggacaccagg atggtcaccc cctgaccgtt gagggtccgg
     2941 aacaattccc gcatcccgat gatgccctcg gggtcgagac cgttggtggg ttcgtcgagg
     3001 atcagcaggg ctgggtcatg cagcagggcg atagcgatgc caagccgctg tttcatgcca
     3061 agggaaaact gcccggcctt cttccctccc gtgtcgccca gccccgtcaa ctgcagcaca
     3121 taggggatcc gctcttccgg gcaacgatag atcttttgca ggacgcggag attctcggcg
     3181 gcggtgagct gggcgtagac cgaggggctt tcgatcattg agcccagttt cttcaggatg
     3241 gagatgcggt gaggggcgaa cggttttcca aagacgctga tctctccctg ctgctttttt
     3301 aagagtccca gcaccagccg gagcgtggtt gttttccccg cgccgttggg ccccaggaac
     3361 ccatagatgc ttccttccgg gacccggaga tcgatcttgt tcagtacgga aacgccggcg
     3421 gagaatcgat gacacagccc ggcggtctcg agacaatagt ttgacataga cccaaaaata
     3481 accgcccgcg agtcacgccg gcaagccatt ctgcaaggcg cctgctttcc tctgcaaaag
     3541 ggggttttac ttctgcgact gtggacgaat ggccgggttg aaccagttaa agagcgcgac
     3601 cgcgacgacg atgacgccat aggccgacag ctgcagggcg tccacttttt ccccatagac
     3661 ggtcaacgcc agcataaagc ccagcacggg attgatatag aggaggatgc cgaccgtaga
     3721 ggacttcagc tgtttcagtg catacaggtt gagccagagc gggaagatgg taagcagcac
     3781 ggcgatgacg gccatgagga tatagaagcg gctttcctgg ggcacagggc catgccaggc
     3841 cgggtaaaag ggcaggagca gcagcgcgga aaaaaggatc tgcccgctca gcaacaggaa
     3901 gcggtcgacg ccatagtcct tccgctggct gacaaggtag agggcatagg agaccgcgat
     3961 cacgaggctg tataccaggt caccgagatg atgaaaggcc agtatcccgc agcccgcggc
     4021 gctcaacagc acggccaccc attggccctt tgtcaaatgc tctttcagga taagccaggc
     4081 cagaacggtc gtcagcacgg ggcagacaag gtaggcaaag gcggcagcct tgatgctcac
     4141 ctggttggtc acataaatga aaaagaacca gttggctgtc aggaacaggc ccccgcccaa
     4201 aagctggaac aacaccccct tctttactgc gtcgggagac gatacgaaga gccggcggtt
     4261 gtccacccac actttccttc gcagcaatac ccctgtcagc agcatcagca gcgcgctgag
     4321 gaatacccgg tagaaaagga ggtcgagaga gggatagacg gcaaggggcc gaagcacaag
     4381 actaaaaaac ccccagatgg taaaagcgga gatggctgcc agataatgcg aagtattttt
     4441 catggtcccg gcaaaattgc gccaaaaaat ggcaaaaaat ggtaaaaaaa agccgctttc
     4501 gcacaatata taaattcccc gtataggttt ctctttaccc gaagtttctg ttatctttcg
     4561 gccaaattca atacctcatg ccgaacctct accctggcat taaaccaact attggtagtg
     4621 gacgatcgta tgtcttcctt tcgattgttc tattcctccc ctttttggtt ctggcacaac
     4681 ccgttatcac cagctttacc ccgcaggcag gagcagtagg ttcgacagtg accatcaacg
     4741 gcgccaattt cggcacaacc cccaccgcca atatcgtttt cttcggctcg gtgcaggcaa
     4801 cggttaccgc cgccaccgcc acctccctca cc
//
LOCUS       DATGFG010000801         5335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1492931, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000801 DATGFG010000000
VERSION     DATGFG010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492931"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2360)
                     /locus_tag="VKQ52_15085"
     CDS             complement(<1..2360)
                     /locus_tag="VKQ52_15085"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88577.1"
                     /translation="MKGSRNIVCVVVVLLGSLLSLPGLGQEPVARKGVIDLRKASLFD
                     KHLKISGEWAFYWNRLLTPDSLSTATPDYIPFPVLWKDVRLHGQPLPSQGYATYALTI
                     LLPPKRPRLGLELHHVYSTYKLFVNGIVQAANGQPATTPDKANPFWVSRTIAIPPNEP
                     DTMVLVMQVANFWHARGGPFKDILIGDKDELFLKKNQDMAYDFMLAGCMLMGGLFFLG
                     LYVFGRKDRTILYFALFCILYSYRMVGTVHYSLHTLVDHLNWFVTIRIEYLTLSLGIA
                     LFSVYTRNLYPEDSHPVVMKAMVWFCLAYSVLILVTPILFFTSLLIFFLGVMFVCIAY
                     AMYVYLRAARHRRSGSLFALLSTAVMLFIFLLGNLNYFGFIPDLRLVLFICYISFFFL
                     QSLALSHRFADSFRQATFQAQQGLRAKSEFLSTMSHEIRTPLNAVIGMTHLLLRNKPR
                     PDQENDLGVLLFSANNLLGIVNNILDYTKIEEGRISIEQIPFDLPAIARNIIAGLQNT
                     ADEKGIALLLDIDSRLDSKLIGDPTRSSQVINNLVHNAVKFTKEGSVRLSLTVEDMTT
                     DATTIKIKVEDTGIGISPEKQNIIFDRFTQADSSTSRSYGGTGLGLAISKKILELQGV
                     DLQVKSEAGKGSCFYYTQTFPVSHESKGEPGTTAASAGLEPLFRDVCLLLVEDNPLNV
                     LVAQTMLENNGARVDVATNGAEALEQLDASRHRMILMDLHMPVMDGYEATILLRQRGE
                     TLPIIALTASMPQEVESEAFAAGLNGVVVKPFSPDELYRVILQHLKP"
     gene            complement(2462..3067)
                     /locus_tag="VKQ52_15090"
     CDS             complement(2462..3067)
                     /locus_tag="VKQ52_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88578.1"
                     /translation="MEQFDISIGQMLVSSPNIIYTIIKSQDIFQGRVVMAGWIPNALM
                     EGSFRVYDDLEKFKKDGEYVNFKEIPIKMGEFQVIGITDLKSTTKPSPIENLIRAFQE
                     IKAIDETKSDFGLIRRVLPEKLRELKAAIDGYVNDNPDKKNDNIEFLIDSSISDCENC
                     PEKFSDDLAEDKWFKTKTIKLAKNAAKNSINILFYPWGVWK"
     gene            complement(3477..4280)
                     /locus_tag="VKQ52_15095"
     CDS             complement(3477..4280)
                     /locus_tag="VKQ52_15095"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HLZ88579.1"
                     /translation="MPQLTLKTPITYYGGKQKLVPYVLPRIPKDHKLYAEPFCGGAAI
                     FFAKDPSEIEVLNDTNRELMNFYRVVQNEFSALEKEIRISLHSRDQHRKASVMYNYPD
                     MFSEVKRAWAVWLLSSQSFSSMLDGSWGFDKTENRTTKLIQVKKERFTEELAIRLQNV
                     QLESADALYVIRSRDSADAFFYCDPPYYNSDMGHYDGYTVEDFEMLLNTLAAIKGRFL
                     LSSYPSPLLLRFAKKKKWHVFSIEQNVSVNAKSGYLKRKVEVLTANYKM"
     gene            complement(4289..5221)
                     /locus_tag="VKQ52_15100"
     CDS             complement(4289..5221)
                     /locus_tag="VKQ52_15100"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HLZ88580.1"
                     /translation="MIKQTQLPGVRNWYGDDIVGIQTELFKVLEGFFGDYGNYVVSGC
                     QVVGSNIRSGIVSIADPVDGPQLAYYPGKNNVSQWPIYISLSKTTTNDTYEDGNNKPV
                     QVTYSAVDSANIPGGNYLVIQQNGAATRIWDVVQNASRRFVTDALVTQWNQNIVQPGM
                     MLYWPKAAAPNGWLECDNTFQAIAAYPNLFAQIGTTWGGDGQTNFRLPPSQGYFIRGW
                     DHGAGVDPGRAFGSIQEDALKSHDHITHAQGRIPGQDGNSGFLSSSVDGRFSGGGKDI
                     FGRDLSPDPNMRTSFTGDTETRPKNIALMLIIKY"
BASE COUNT         1314 a         1232 c         1411 g         1378 t
ORIGIN      
        1 ggtttcagat gctgcaggat cacccggtat aattcatcgg ggctaaaggg ttttacgacg
       61 acgccgttga ggccggcggc aaaggcctcg ctttccactt cctgtggcat gctggcggtg
      121 agggcgataa tcggcagggt ctctccgcgc tgccgcagga ggatcgtcgc ctcgtagccg
      181 tccattaccg gcatgtggag gtccatgagg atcatccggt ggcggcttgc atcgagctgc
      241 tcgagggctt ccgccccgtt ggtggccacg tcaacccggg cgccattgtt ctccagcatc
      301 gtctgggcca ccagtacgtt cagggggtta tcttctacca gcaacaggca gacatcgcgg
      361 aacaggggct ccagaccggc ggaggctgct gtcgtcccgg gttccccctt gctttcatgg
      421 cttaccggga aggtttgggt ataataaaaa caggacccct ttccggcttc gctttttacc
      481 tgcagatcga cgccctgtaa ttccaggatc ttttttgaaa tggcgaggcc gagacccgtg
      541 cctccgtagc tgcgggaggt ggacgagtcg gcctgggtaa accggtcgaa gatgatattt
      601 tgtttttccg gcgagatgcc gatgccggta tcttcgacct ttattttaat agtggtggca
      661 tccgtcgtca tatcctccac ggtgagggat aggcgaaccg agccttcctt cgtgaatttg
      721 accgcgttgt ggacgaggtt gttgatcact tgactggagc gggtggggtc gccgatcagc
      781 ttgctgtcga gccggctgtc gatgtcgagg agcagggcga tccccttttc atcggcggtg
      841 ttctggagcc ctgctatgat gttccgggcg atggccggca gatcgaacgg gatctgttcg
      901 atgctgatcc taccctcctc gatcttggtg tagtcgagga tattgttgac gatgcccagc
      961 agattattgg ccgagaaaag caataccccg agatcgttct cctggtcggg ccggggtttg
     1021 ttgcgcagca ggagatgggt catcccgatg acggcattca gcggggtgcg gatctcgtgg
     1081 gacatcgtgg aaaggaattc ggatttcgcc cgcagaccct gttgggcctg gaaggtcgcc
     1141 tggcggaagc tgtcggcgaa gcgatgggac agcgcgaggg attgaaggaa gaaaaaggag
     1201 atatagcaga tgaagagcac cagtcgcagg tcggggataa aaccaaaata gttgaggttg
     1261 ccgagcagga agatgaacaa catgacggct gtgctgagca gtgcgaacag agacccgctg
     1321 cgccggtggc gggcggcgcg gaggtagaca tacatggcgt aggcgatgca aacgaacatg
     1381 accccgagga agaagatcag gaggctggtg aagaaaagga tcggggtaac gaggatcagg
     1441 acggagtagg cgagacagaa ccagaccatg gccttcatca cgacggggtg ggagtcctcg
     1501 ggatagaggt tgcgggtata gacggaaaaa agggcgatgc ccagggacag ggtcaggtat
     1561 tcgatccgga tggtgacgaa ccagttcagg tggtcgacca gcgtgtggag ggaatagtga
     1621 acggtgccga ccatgcgata gctgtacagg atacagaaca gggcgaagta gaggatcgtt
     1681 ctgtctttcc ggccaaatac atagagcccg aggaagaaca gaccgcccat gagcatacag
     1741 ccggccagca tgaagtcata ggccatgtcc tggttcttct tcaggaagag ttcgtccttg
     1801 tccccgatga ggatgtcttt gaacggtccg ccgcgggcat gccagaagtt tgccacctgc
     1861 atcacgagaa ccatggtatc cggttcgttt ggcggaatgg cgatggtcct gcttacccag
     1921 aagggatttg ctttatcggg ggtggtagcc ggctggccgt ttgccgcctg cacaataccg
     1981 ttgacgaata gtttataggt gctgtagacg tggtggagct ccaggccaag ccggggtctt
     2041 tttggcggaa ggaggatagt aagggcgtag gtggcatagc cctgggatgg gaggggttgt
     2101 ccatgaagcc ggacgtcctt ccagaggacg ggaaagggga tatagtcggg agtggcggtg
     2161 gaaaggctgt cgggggtgag gaggcggttc cagtaaaatg cccattcccc actgatcttc
     2221 aagtgcttgt caaagagaga agcctttctt agatcgatga cacctttgcg ggctacgggt
     2281 tcctgtccga gccccggaag actcaaaaga gagcccagga gtaccactac gacacaaact
     2341 atattcctcg atcctttcat aaaagacgga cagtgatgct actaatataa tcgcaaaaac
     2401 gaaaagtcgt aatttttaaa acgaaatgtc caatttggaa tgcaggaggc cgtgtactat
     2461 tttacttcca tactccccag ggataaaata gaatattaat ggaatttttg gctgcatttt
     2521 tggccaattt tattgttttg gttttgaacc atttatcctc agctaaatca tccgaaaact
     2581 tttcgggaca attctcacag tcagaaatac tggagtcaat taagaactcg atattatcat
     2641 tttttttatc gggattgtcg ttaacatatc catcaattgc agcttttagc tccctcagct
     2701 tttcgggtaa aactctcctt attaacccaa agtccgattt tgtttcatct attgccttta
     2761 tttcctggaa tgcccttatt aagttttcaa ttggagatgg ttttgttgta ctttttaaat
     2821 cggtgatccc aataacctga aattcgccca ttttaatggg aatttcttta aagttcacgt
     2881 attcaccatc ttttttgaat ttttccaaat catcatatac tctaaacgat ccttccatta
     2941 atgcattggg tatccaacca gccataacta ccctgccttg aaatatatct tggctcttta
     3001 taattgtata aataatattc ggggatgata caagcatttg tccaattgaa atatcgaact
     3061 gttccataaa ataatgattt aggtggttaa actgtaaaga ttttgattcc aataaatgca
     3121 gatagcttcc aatatacctc atttgctaat aaatgcaaaa attggtaatg tctcaatttg
     3181 aaaaatgttg taacttgtaa tattaaattt gacctaatgt gcagagaata cccaaaagtt
     3241 gtttatcgag agctaataaa aatagaagct acacgtatag aggtgtatga gaaatttgca
     3301 aatggaactg atggaatttg tatatggagt ttcatcgatg gaaattggcg gcgataaaaa
     3361 gagtaaaacc cggctgcaac cgggttttaa atattttcat tccctcctgg aactgactat
     3421 taaaattact aaaaattagt cagtatagga aaatgggtaa ttggtatcac tagttattac
     3481 attttataat tagccgtaag aacctcaacc ttccgtttca aataccccga tttcgcattc
     3541 acacttacat tctgctcaat agagaaaacg tgccacttct ttttcttagc gaaccgtaat
     3601 aaaagcggcg aaggataaga ggaaagcaaa aaccggcctt tgattgctgc cagggtgttg
     3661 agcagcattt caaaatcctc tacggtatag ccatcataat gccccatgtc tgaattataa
     3721 tagggaggat cacaataaaa gaaggcgtcc gcgctgtccc ggctcctgat cacatatagg
     3781 gcgtctgcgc tttctaactg gacattttgc aaccggatgg ctaattcttc cgtgaaccgc
     3841 tcttttttta cctggatgag tttggtcgtt ctgttttcgg tcttatcgaa gccccaggag
     3901 ccgtccagca tagacgaaaa ggactgagaa gatagcaacc agacggccca ggcgcgtttt
     3961 acttcgctga acatatccgg gtaattatac atgacggacg ctttccggtg ctggtcacgg
     4021 ctatgcaggg aaatacggat ttctttttcc agggcggaaa attcattctg gacaacccgg
     4081 taaaagttca ttaattcccg attggtgtca ttcagcacct ctatttcgga agggtctttt
     4141 gcaaagaaga tggcagctcc gccgcaaaac ggttcggcat acagcttatg gtctttcgga
     4201 atccgtggca atacataggg gactaacttc tgtttaccac cataataagt aatgggcgtc
     4261 tttagtgtta attggggcat aactacaatc agtatttaat tatgagcatg agcgcgatgt
     4321 tcttcgggcg ggtttcggta tctccagtaa atgacgttcg catattaggg tcaggactta
     4381 ggtctcggcc gaaaatatcc ttgccgccgc cggagaaacg gccatcgacg gaactggaga
     4441 ggaagcccga gtttccgtct tgccctggta tccttccctg ggcatgggtt atatgatcgt
     4501 ggcttttcaa agcatcttcc tgaatgctac caaaagcccg tccaggatca acgcctgccc
     4561 cgtggtccca accccttatg aaatagccct ggctcggagg caagcggaaa tttgtctgcc
     4621 cgtcccctcc ccaggtcgtt ccgatctgcg caaacaggtt gggataggca gcaatcgcct
     4681 gaaaggtatt gtcgcactcc agccaaccgt tcggggcggc ggctttcggc caataaagca
     4741 tcatacccgg ctgtacgata ttctgattcc attgcgtaac gagggcatcc gtgacaaagc
     4801 gccgggaggc attttgcacg acatcccata tccgggtcgc cgctccgttc tgctggatca
     4861 ccaggtaatt cccgccgggg atgttcgcgg aatctacggc gctataggtg acctggaccg
     4921 gcttattatt gccatcctca taggtatcat tcgtggtggt tttgctcagg ctgatatata
     4981 tcggccactg gctaacatta tttttgcccg gatagtaggc gagttgcggc ccgtctacag
     5041 gatcggcaat gcttacaata ccggacctta tattgctgcc cacaacctgg cagccggata
     5101 ctacataatt accatagtcc ccgaagaagc cctccagtac cttaaatagc tccgtttgga
     5161 taccgacaat atcgtcgccg taccaattac gaacccctgg taattgcgtt tgcttaatca
     5221 ttagctggtt ttggttttta tgtcatagct gcggcctgcc agcttgtatt tgttgacgat
     5281 gccctgtact tgcttggcat ctacggaccc cggaataata acccggaagt catac
//
LOCUS       DATGFG010000802         5426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_587867, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000802 DATGFG010000000
VERSION     DATGFG010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587867"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..2727
                     /locus_tag="VKQ52_15105"
     CDS             16..2727
                     /locus_tag="VKQ52_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ88581.1"
                     /translation="MACKKPGSHPTLFRITLYRKKRAAGSPKFAKCQRPQIAGSFEEG
                     SAKKKPPIAPSNLLGYVLSENGDTLAYATIKFASAPGSPARTYAISSAHGFFTLQLPP
                     DGKGFLEVSAVGYRPRIVATDSIPRGRSFPILLSRPDHELPPIVIKSEPAIRISGDTI
                     AFTAAAFTRGNEQSIGQLLSNIPGFTVQENGKISFNGEPIDKILLEGDDLTGKSYEML
                     VNKLRPAGIDKFEVLRNVQDPENITSGMVKTNRQVLNLKFKKSFLGRLFGNIEASGGL
                     PTTVYSLDAQAVSLLKKSRLVTLGDANTKGDIGIPATETNTWQRPEEKDDITQITPTD
                     NTPLANIDDIKSAYLKKPLIAANHTQQFSINGFSRLGRQLILKANTRFISDDYDQDVL
                     SIKNIFTPTQTLVTNQSQAIHKKNTLFSNTISLNYIGKKGSQLALVLMNSNRGSIHFR
                     NGVLNELDYADKTDGRNDQYNGKLTWQHLFKRSAALGFSVQENRNTLPNTYTLTTHST
                     ILQQERQQFSSLTVNGFIIKRLSRHSITLTATYRHEKDKLFTSIDTTAPADVNNNTAL
                     AREMSLELRDVWTCSPKLSLTLSGSLLYFTYSLADKSLDKPLLLPNLSINYQLSRLDK
                     FTLSYTAKNSFAELRYNTAGYIITGATGYMKGNDTLKSGIGHSAQLLFSHLDLLDHRW
                     LFFVGAVLQDNPYLYLASQTPNAYYTYSAYTTTGQRLITTAFFSHADKFFSDMHSKIS
                     PNLNLSRVYTSSIMDGQPSTNTITQLKMGTDLTAAIRSVDIAGGFSYSLSGEKNTSGN
                     KEETQTHEMDIHLDLTWKIATGVFFSGNTAWSRVIPPGVQPINTIASAAGIDFRPGEG
                     KWEYGVQCTNVFNQRAFSYSAITPLQTSLQQYRLFPRCLSARLRYTF"
     gene            3286..3663
                     /locus_tag="VKQ52_15110"
     CDS             3286..3663
                     /locus_tag="VKQ52_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88582.1"
                     /translation="MADLKRRTLQLSSGKQIKLFGNSVAISKTLEIGEGYAPNILSAI
                     EAQPENKIPAGISNPHRLTVEELMELADFNIQLWMDLKAGLRKYGQLDAKIFGQEAAK
                     TPAPGEDKPGAGSRRKNAEKESQ"
     gene            3809..4168
                     /gene="mobC"
                     /locus_tag="VKQ52_15115"
     CDS             3809..4168
                     /gene="mobC"
                     /locus_tag="VKQ52_15115"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HLZ88583.1"
                     /translation="METVRMKKKKGGRPPKKLKRELRVTVRFSRLEHYILEQKATKAG
                     MNVSEFIRPAAIYGKVIARQTEEERHFTRQLVGMANNFNQMAKVANREGIASTRLLLE
                     SCLQQAHGLLYKLQHGE"
     gene            4158..5117
                     /locus_tag="VKQ52_15120"
     CDS             4158..5117
                     /locus_tag="VKQ52_15120"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HLZ88584.1"
                     /translation="MVSNVFPGHSFYHAGRYVSKKPGAELLDYQDVRGYDYKLMAEDF
                     IAQSKLRPSKEKACFHAALSFYPGEKPSDETMIQIAREYLEELGIVNTQYAIVKHTDR
                     AHLHLHILANMVDNDGKPIKDGWIGLRGKKVAQRLTRKYDLIPAEGKHLDKTHLEALS
                     QSEVNRYKIYQAIVDNLPHCRSLPDLEKRLIAQGIHTQYKYKGQTQELQGISFRIGKD
                     RFKGSQIDRKFSLGSLQKTLASQRQSQETKPALSLEARKKAILIRGNSQKPAAPAPQY
                     NQGPPPLLVQLMTHIAAAGDVPYEQTAAFVPQKKKKKKKGLGR"
BASE COUNT         1535 a         1462 c         1251 g         1178 t
ORIGIN      
        1 gctaaccgac ctggaatggc ctgcaaaaaa cccggtagcc atccaaccct gttccggatc
       61 accctttatc gcaaaaaaag agctgcagga tctccgaaat tcgcaaaatg ccaacgccct
      121 caaattgctg gaagcttcga agaaggatct gcaaaaaaaa agccgccaat agcgccatcc
      181 aacctcctcg gttatgtcct tagcgaaaac ggagataccc tagcttatgc caccataaaa
      241 tttgcttctg ctcccggttc gccggcaaga acctatgcca tatcctccgc gcatgggttc
      301 tttaccctcc agctgccgcc cgacggcaaa gggtttttag aggtcagcgc cgtaggttat
      361 cgaccccgga tcgtagccac cgatagtata cccagggggc ggtccttccc gatcctcctc
      421 tcccgcccgg atcacgaact ccctccgatc gttataaaaa gcgaacccgc catccgcatc
      481 tccggtgata cgatcgcctt taccgcggct gcctttacaa gaggcaacga acaaagcatc
      541 ggtcagctgc tctccaatat ccccggattt accgtccagg agaatgggaa gatcagcttc
      601 aatggcgaac ccatcgataa aatactgttg gaaggagacg atctgacagg taaaagttac
      661 gagatgctgg taaataaatt aagaccggcc ggcatcgaca aattcgaggt actaaggaat
      721 gtacaggacc cggagaatat cacttcgggg atggtcaaaa cgaacaggca ggtgttgaat
      781 ctgaaattta aaaaaagctt cctgggcagg ctgtttggaa atatagaagc atcagggggc
      841 cttcccacaa ctgtttacag cctggatgcc caggcggtct ccctcctgaa aaaatcccgg
      901 ctggtgaccc tgggtgacgc caatacaaaa ggcgatatcg gtataccggc aacggaaaca
      961 aacacctggc agaggcccga agagaaagac gatattaccc agatcacccc caccgacaat
     1021 actcccctgg ccaatatcga cgacataaaa tccgcttatt taaaaaagcc gttgatcgcg
     1081 gccaatcaca cgcagcaatt ctccatcaac ggtttttccc gtttggggcg gcagcttata
     1141 ctaaaagcca acacccgttt catcagcgat gattacgacc aggacgtttt atccataaag
     1201 aacatcttta cccccaccca aacccttgtt accaaccaat cacaggcaat tcataaaaaa
     1261 aacaccctct tctccaatac gatctcgttg aactacatcg gcaaaaaagg cagccagctg
     1321 gccctggtgt tgatgaacag caacagggga tcgatccatt tcagaaacgg agttcttaac
     1381 gagcttgact atgcggacaa aaccgatggc cgcaatgatc aatacaatgg gaaactgacc
     1441 tggcagcatc ttttcaaaag atctgcggcg cttggatttt ccgtacagga gaataggaat
     1501 accctaccca atacctatac actgaccacc cacagtacta tattgcagca ggaaagacag
     1561 caattctcct cgctgaccgt gaatgggttt atcataaaac ggttgtcccg ccatagcatc
     1621 accctaaccg ccacctaccg tcacgaaaag gataaactct ttacttccat cgacaccaca
     1681 gcccccgccg atgtcaataa caatacggcc ctggcaaggg aaatgagcct cgagctgcgg
     1741 gatgtatgga catgcagccc caaactctcg ctgaccttgt ccggcagtct tttgtatttt
     1801 acgtactccc ttgccgacaa atccctggac aagcccctgc ttctacccaa cctgtccata
     1861 aattatcagc tatccaggtt ggacaaattc accttatcct ataccgcaaa aaatagtttc
     1921 gcagagctgc gttataatac cgccggctat atcatcacgg gggcgaccgg ttacatgaaa
     1981 gggaatgaca cgctgaagtc gggtatcggc cattccgccc agcttttgtt ttctcatctg
     2041 gatctcctcg atcatcgctg gttatttttt gtgggggcgg tactccagga caacccatac
     2101 ttatacctgg ccagccaaac acccaatgcc tactatacct attccgccta tacgaccacg
     2161 ggtcaacgac tgatcaccac cgcgtttttt agtcatgcgg ataaattctt ttccgacatg
     2221 cattcaaaaa tatcacccaa tctaaacctt agccgggtgt atacgtccag tatcatggac
     2281 ggtcaaccat ccaccaacac aataacccag ttaaaaatgg gaacagatct tacggcggct
     2341 atccgttcgg tggacatagc aggcgggttc agctattcgt tgtccggtga aaaaaatact
     2401 tccggcaaca aagaggagac gcaaacccat gaaatggata tacacctgga ccttacctgg
     2461 aagatcgcaa ccggcgtctt ttttagtggg aataccgctt ggagccgggt cattcccccg
     2521 ggagtacaac cgatcaatac catcgcatcc gccgcgggta tcgacttccg tccgggtgaa
     2581 gggaaatggg aatatggggt gcaatgcacg aatgtattca atcagcgggc tttctcgtat
     2641 tcggccataa cacctttaca gacctcgctt cagcagtaca ggctcttccc ccgctgtctt
     2701 tccgcccgtc ttcgctatac gttttgatat aaaagggggg aataaatcgt ttgcgccccc
     2761 ggaaatgcaa aacccgcaac tttcgctgcg ggcttcctgt gagtcgccct gcctgtacga
     2821 ttttcgaact tactcatcca tgatttaagg aagcttgcca atctagcggt ttgaaaacca
     2881 atgttttcag ccgctttttt tattaccgtt catcaaccgc ccaatttatg aaaattacta
     2941 atccgttatt ccagccctat aaatgaggtc tcccggagag catccaaggg aatcgatcca
     3001 taatgtgtga aatgaaacag tgagatatga ataactggac ggacaatctt atatacctct
     3061 tttggacggc ttatccacct ttaggccgct cagctcatca ttcaaaaaaa gagaggttcc
     3121 ccggaggggg ttcccctttg tggtgccccc tccggggttt gggggctttc tttccccgta
     3181 agagcctcaa atcaccagcc atccgcggcc ccacttgccc ccacaatccc ggcgcatgcg
     3241 accggatcaa ttctaattat ttcatcattt taaaatacta cgatcatggc agacctgaaa
     3301 agaagaaccc tgcaattaag cagcggcaag caaataaaac tattcggcaa tagtgttgca
     3361 atcagcaaaa ctttggagat cggggagggc tacgccccga atattttatc agcaatagag
     3421 gcacaacctg aaaataagat cccagccggt atctcaaacc cgcataggtt aaccgtggag
     3481 gaactgatgg aactggcaga tttcaatatc caattgtgga tggatttgaa ggccgggctt
     3541 cgcaaatacg gacaactcga tgcgaagatt ttcggccagg aagctgcaaa gacacctgcc
     3601 ccaggcgaag ataagccagg agccgggagt agacgcaaaa atgcggagaa ggaaagccaa
     3661 tagtgccaaa gggctttcgg agtttttagg gcgagcggtg ttgttgtcgg acaaaaagtc
     3721 ctccaacaac gctcgctttt tgctcccgtt ggtcgcaaaa agaggccggc ccgaaggccg
     3781 gcagaaaaac aacctaaaaa agtgagatat ggaaacagtg cggatgaaaa aaaagaaagg
     3841 gggaagaccc ccgaaaaagc tcaaaagaga gctacgagtt accgtccggt ttagccggtt
     3901 ggaacattat attcttgagc agaaagctac caaagcaggg atgaatgttt cggaatttat
     3961 ccgaccggct gctatttacg gtaaagtaat agcccggcaa accgaggaag aaagacattt
     4021 tacccggcag ttggtgggga tggcgaataa ctttaaccag atggcaaaag tcgctaacag
     4081 ggaagggatc gccagcactc gccttctgtt agaaagctgc ttgcagcaag ctcatggcct
     4141 tctatataaa ctacaacatg gtgagtaatg tatttcccgg tcattccttt taccacgcgg
     4201 gtcgctacgt ttccaagaaa ccgggggcgg agctgctgga ttaccaggat gtaagaggat
     4261 acgattataa attgatggcc gaggatttta ttgcacaaag taaactccgt ccttccaaag
     4321 agaaggcttg ttttcacgcg gccctgagtt tctaccctgg agaaaaaccc agtgacgaaa
     4381 ccatgataca gatcgcccgc gaatacctgg aggaactggg tatcgtcaat acgcaatacg
     4441 ccatcgtaaa acataccgac agggcgcact tgcacctgca tatcctggct aatatggtag
     4501 acaacgacgg caagccgatc aaagacggct ggatcggtct acgaggaaaa aaggtcgccc
     4561 aacggttaac ccggaagtac gaccttatcc cggcagaagg caagcacctg gataaaaccc
     4621 acctggaagc actcagccag tcggaggtaa accggtacaa gatttaccag gccatcgtag
     4681 acaatttacc ccattgtcga agcctgcccg atctggaaaa gagattgatc gcccagggta
     4741 tccataccca atacaagtat aagggacaaa cgcaggaatt gcagggaatc agtttcagaa
     4801 tcgggaaaga tcgtttcaag ggaagccaaa tagaccggaa attctccctg ggcagcctgc
     4861 aaaagacact tgcttcccaa cggcaaagcc aggaaaccaa accggcactt tctttggagg
     4921 cccggaaaaa agccatcctc atccggggca actcccagaa accagccgca cccgctcccc
     4981 aatataacca gggaccacct ccattgttgg tgcaactaat gacccatatt gcagcagcgg
     5041 gagacgttcc ctacgagcag acggccgcat tcgtccccca aaaaaagaag aaaaagaaga
     5101 agggtttagg gcggtaatct ttgccgtata gattcggggc gcactgcgtc ccgaattgac
     5161 gacagggtcc ggtacatgca gccgcagccg atcagcggaa gaatattgcc gggctcccgt
     5221 accggctttg gcccttcgcg gcgatacatc attgccgggg cttctccccc gaatcaacta
     5281 ttcggggcca ttcggcaaat gaccattgtt cacccatgaa tggggcaatg atgtatcccc
     5341 gcgaagtttg gttttccctt tcccccctat ttctctaccc tggcaggccg cgccggtcat
     5401 tttttttcgg ctaatgcaaa ggcaca
//
LOCUS       DATGFG010000803         5589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1275442, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000803 DATGFG010000000
VERSION     DATGFG010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275442"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VKQ52_15125"
     CDS             <1..228
                     /locus_tag="VKQ52_15125"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HLZ88585.1"
                     /translation="MNIDTDLQWAFWEGVQQYYKKNEGYLQGQLGNPEGADKPNKKYY
                     DPRVWLRKGEESFIRRLEVAFEDLNCINRNA"
     gene            342..1406
                     /locus_tag="VKQ52_15130"
     CDS             342..1406
                     /locus_tag="VKQ52_15130"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HLZ88586.1"
                     /translation="MPSKKILDLLGDKASFLLDHKPVIDKSTLSAFPSPNHVEEIWVN
                     SDRNNQVLRNIQVLLGHGRLADTGYLSIFPVDQGVEHSAGASFSANPMYFDPENIIKL
                     AIEAGCNAVASTFGVLSIMSRKYAHRIPFVVKINGNEFLSLPNRYDQTLFGTVKHAWN
                     MGAVSVGATVYWGSPESDRQLKEVAEAFELAHELGMATILWCYTRNAGFKVDGVDYHT
                     SADLTGQANHLGVTLRADIIKQKLPTNNGGYLATKHGRTSPLVYSKMTHPDNHPIDLT
                     RYQVINCYSGRVGLINSGGESKGDSDLADAVFTAVVNKRAGGMGMIMGRKAFQRPFNE
                     GVELINAVQDVYLDKSITIA"
     gene            1497..3209
                     /locus_tag="VKQ52_15135"
     CDS             1497..3209
                     /locus_tag="VKQ52_15135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLZ88587.1"
                     /translation="MHQPCRPLLILLLICLACGPAFAFPDADTTRPNIPDPTKLPPDS
                     IGTRLLREGRKNREAGQYTPAMDNDLQALHIFQQTGNRSREASVNTEIAQLYQYMGEQ
                     KSSPSLVRQGLDYVREGLDLYIAVKDTAKEVAARNTQGVIYRSMALVDRAYWYDSAMA
                     CYQAALARITPSGKGKRTTGALYNNISQVYSEYKKDYPAALHYLEEAVRFNAGQPDSA
                     FNLSYNYGNIAAVYQKMGDKKRSLEYAYKTLDMARRVGTANRLLNAYEQLYNSYDQFG
                     KADSAFHYYFIFDNMRDSMASLTTTRQIAEAQTKYETEKNKALITELNNRNNLQQKSI
                     IVLLAGLAVVLLFSAGLFALFRRVQRQKQLITTQSGQLEVMMKELHHRVKNNLQIISS
                     LLSLQSYRLKDEEALEAIRLSQQRVQAMSFIHQRLYTSDQARMVNMKEYLQDLTLSLV
                     MAYGYNQNTLDLDVQIARQWLDVDKALPLGLIANEITTNALKYAFQGDQRPALSVGLA
                     EEGGQLLFTVKDNGRGWDVRQWEHPGASFGRQLVETLCRQLKATQKLTYLNGSVFAFA
                     IPLE"
     gene            3265..4053
                     /locus_tag="VKQ52_15140"
     CDS             3265..4053
                     /locus_tag="VKQ52_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HLZ88588.1"
                     /translation="MRILIVEDEPIIALDLSTGLQQFGYIITGIAEDEPSAVGLFTEK
                     ETDIVLMDINLKGEKDGVFTALQLLKIRSVPVIYLTAFTDATTVQRVKQTHPAAFLSK
                     PYTVQNVQIAIELALHNFTAGKTPHQDRPPEPAPDPQRGPGPDLQRGPAPDSLFQWND
                     HLFVKVNYRFIKIALAAVLYLAAENNYIHLVTSEKKYLLRLSLQQFMDKIACDKLVRI
                     HRSYIVHLDAIQSFTEQEVTIDKEVLPVGRQYRDSFLQQFGWKG"
     gene            complement(4265..5443)
                     /locus_tag="VKQ52_15145"
     CDS             complement(4265..5443)
                     /locus_tag="VKQ52_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLZ88589.1"
                     /translation="MAQNPTGTLVPPEKLVEDTTEQRDIIGVVLKFTHIHIKKQPKVA
                     GQRVYYTFIPLGTSVPGGGEALITATNAAFNLGDPKTTFLSNVTFSPSTNLRGEWNIP
                     FRSNVWSPGNKWNFSGDYRLTFFPQFTWGLGGNTAPSKKILLRYTYVRFYQNALWSIA
                     NKPFLFAGLGYNLDYHIHIRPDNDTIDIQKFTGYTYGTANHTNSFSSGITLNLLYDTR
                     NNSLNPLPGWYYNIVARVNPKVLGSQENWYSFYFDARKYISFDPNSWNVLAFWSYLWT
                     TMGSYAPYLDLPAITWDANQRSGRGFYPSRYTGRSLWDFETEYRRSITRDELLGFVVF
                     GNMNSVTEPTSHQFAYLHAAAGAGLRIKFNKHSGTNFGMDFAASKGYWALYFGLGEAF
                     "
BASE COUNT         1397 a         1746 c         1343 g         1103 t
ORIGIN      
        1 atgaatatcg acaccgacct gcaatgggcc ttttgggaag gtgtacaaca atactataaa
       61 aagaacgagg gttacctcca gggtcaactc ggcaatcccg aaggagcgga caaacccaat
      121 aaaaaatact acgacccccg cgtctggtta cgcaaaggcg aagagagttt catccgtcgc
      181 ctggaagtgg ccttcgaaga ccttaactgc atcaaccgta acgcgtaggt taccccattt
      241 gtacatgtca cggttccttt tgccggtctt accccggcag gaggaacttt ttttcttttg
      301 gaagcatcgg gttaaattgc ggtacattaa atgtcaatat catgccttcc aagaaaattc
      361 tcgacctcct cggcgacaaa gcctccttcc tgctggatca taagcccgtc atagacaaat
      421 caacgctcag cgcctttcct tcgcccaacc atgtagaaga gatctgggtc aactccgacc
      481 ggaataacca ggtattgaga aacatacagg tcctgcttgg ccacggaagg ctggccgaca
      541 cgggctattt atccatcttc cccgtcgacc agggagtcga acatagcgcc ggcgcctcct
      601 tctccgccaa tcccatgtac ttcgatccgg aaaatatcat caagctggcg atagaagcag
      661 gctgcaacgc agtagcctcc accttcgggg tcctcagcat catgagccgc aaatatgccc
      721 accgtatacc cttcgtcgta aagatcaacg gcaacgaatt cctgagcctg ccgaaccgct
      781 acgaccagac cctgttcggc acggtaaaac acgcctggaa catgggcgcc gtttccgtgg
      841 gcgccaccgt ctactggggc agccccgaaa gcgaccgcca gttgaaagag gtcgcggaag
      901 ccttcgagct ggcgcacgag ctgggtatgg ccaccatcct ctggtgctat acccggaatg
      961 ccggcttcaa agtggacggc gtagactacc acacctccgc cgacctcacc ggccaggcca
     1021 accacctcgg cgttaccctc cgcgccgaca tcatcaaaca aaaactgccc accaataacg
     1081 gcggctacct tgccaccaaa catggacgca cctctcccct ggtgtactcc aagatgaccc
     1141 accccgacaa tcaccccatc gacctcaccc gctaccaggt gatcaactgc tacagcggtc
     1201 gcgtagggtt gatcaacagc ggcggcgaaa gcaagggcga ttccgatctg gcagatgccg
     1261 tcttcactgc ggtcgtcaac aaacgcgccg gcggcatggg catgatcatg ggccgcaaag
     1321 ccttccagcg ccccttcaat gaaggtgtcg aactgatcaa cgccgtccag gacgtctacc
     1381 tcgacaaaag catcaccatc gcataggatc tacgtgtgga tataggttcc ctcttataca
     1441 agcttgaggc cgtctacttt taacctgaga cggcctcttt ttttttatct ttaggcatgc
     1501 accagccctg tcgccccctt ctcatccttc tcctcatctg ccttgcctgt ggccctgcat
     1561 tcgcttttcc tgacgccgac acgacccggc ccaacatccc cgacccaaca aaactgcccc
     1621 cggacagcat cggcacgcga ctgctgcggg aaggcagaaa aaaccgggaa gcaggccaat
     1681 acacccccgc aatggacaac gacctccagg cccttcacat cttccaacag accggcaacc
     1741 ggtcccggga agcatccgtc aacaccgaga tcgcccagtt ataccagtat atgggggaac
     1801 agaagagcag cccctccctc gttcgccagg gactggatta tgtccgggaa ggcctcgacc
     1861 tctacatcgc cgttaaggac accgcaaagg aggtcgccgc caggaacacc cagggcgtca
     1921 tctaccggtc gatggcgctc gtggaccgtg cctactggta tgactccgcc atggcctgct
     1981 accaggccgc actcgccagg atcaccccct ccggcaaagg caaacggacc acgggggccc
     2041 tatacaacaa tatcagccag gtatactcgg aatacaaaaa ggactatccc gccgccctcc
     2101 actacctcga agaagcggtc cgcttcaacg caggacaacc cgattccgcg ttcaacctgt
     2161 cctataacta tggtaacatc gcggccgtgt accagaaaat gggcgacaaa aaacgatccc
     2221 tggaatacgc ctataaaacg ctcgacatgg cgcgtcgcgt cggcaccgcc aatcgcctgc
     2281 tgaatgccta cgaacaattg tacaacagct acgatcagtt cggcaaggcc gactccgcct
     2341 ttcactatta cttcatattc gacaacatgc gggattccat ggcctcgctg accacgaccc
     2401 ggcagatcgc ggaggcccag acaaaatacg agacggaaaa gaacaaggcc ctcatcaccg
     2461 agctcaacaa ccgcaacaac ctccagcaaa aaagcatcat cgtcctcctg gcaggcctcg
     2521 ccgtcgtgct gctcttctcc gccggcctct ttgccctctt ccggcgggta caacggcaaa
     2581 aacagctgat caccacccag tcgggacagt tggaggtcat gatgaaggaa ttgcatcatc
     2641 gcgtgaagaa caacctccag atcatcagca gcctcctgag cctgcaaagc tatcggctaa
     2701 aggatgagga ggcgctcgaa gccattcgcc ttagccaaca gcgcgtacag gccatgagct
     2761 tcatccatca gcggctctat acgtccgacc aggcccggat ggtcaatatg aaggaatacc
     2821 tgcaggacct caccctttcc ctcgtcatgg cctacggata taatcaaaat accctggacc
     2881 tcgacgtaca gatcgcccgt caatggctcg acgtcgacaa agccctgccc ctgggcctca
     2941 tcgccaacga gatcaccacc aacgcgctca agtacgcctt ccaaggcgat cagcggccgg
     3001 ccctgtccgt cggtctcgcg gaagaagggg gacagttact cttcaccgtt aaagacaatg
     3061 gccggggatg ggatgtccgc caatgggaac accccggtgc ttcctttggc cggcaactgg
     3121 tagaaaccct ctgccggcaa ttaaaagcca cgcagaagct gacctacctg aacggttcgg
     3181 tcttcgcctt cgctatcccc ttggaatagt tgtgaattac ccctacctta aacgcgaaag
     3241 aaaataatca tgccggaaga gtccgtgaga atattgatag tagaggatga acccatcatc
     3301 gccctggacc tgtccaccgg cctgcaacag tttgggtata tcatcaccgg gatagccgaa
     3361 gacgagccct cggcagtcgg gctttttaca gagaaagaaa cggacatcgt cctgatggac
     3421 atcaacctga aaggagaaaa agacggggtg ttcaccgcgc tgcaactgtt gaagatccgc
     3481 tccgtgccgg tcatctatct gacggctttc acagatgcaa ccactgtgca gcgcgtgaaa
     3541 cagacccacc ccgcggcttt cctgtccaag ccatataccg tccaaaatgt tcagatcgca
     3601 atcgaactgg ccctgcataa tttcacggct ggtaaaaccc ctcaccagga ccgccccccg
     3661 gaacccgcac ccgatccaca acgcggaccc ggccctgatc ttcaacgcgg acccgccccc
     3721 gacagcctct tccagtggaa cgatcacctc ttcgtcaaag tcaattaccg cttcataaaa
     3781 atagccctcg cggctgtttt gtatctcgcg gcagagaaca actatatcca cctcgtcacc
     3841 agcgaaaaaa aatacctcct tcgcctttcc ctgcaacagt tcatggacaa gatcgcctgt
     3901 gacaaactcg tccgtatcca tcgctcctat atcgtacacc tggacgccat ccagtccttc
     3961 acggaacagg aagtgaccat cgataaagag gtccttcccg taggccgcca ataccgcgat
     4021 tctttcctgc aacaattcgg ctggaaggga tagtgggaaa aaataaaatg aggaggtcgg
     4081 aaccctctga catttccgac ctgccccatt aaaccggccc gggccggctc cacttgcgta
     4141 aaaccggaac aaagctactc caccgaatgc cgctgacaac cgggatgtat gtcaacaacc
     4201 ggaatttgta tgggaacaac cggatttcag gggtcggccg atccaccccg cggcgcggcg
     4261 acactcagaa cgcctcgcca aggccaaaat aaagcgccca ataccccttg ctagccgcaa
     4321 agtccatccc gaaattggtc ccggaatgct tgttgaactt aatgcgtagt cccgcgcccg
     4381 ccgccgcatg caggtacgcg aattgatgac tcgtaggctc cgtcaccgaa ttcatattgc
     4441 cgaacaccac aaaccccaat aactcatccc gggtgatcga ccggcgatat tccgtctcga
     4501 aatcccaaag actccgtccc gtataccggc tgggatagaa ccccctgccg ctacgctggt
     4561 tcgcatccca ggttatcgcc ggcagatcga gatacggcgc atacgatccc atcgtcgtcc
     4621 acagatagga ccaaaatgcc agtacattcc agctgttggg atcaaagctg atgtatttcc
     4681 gcgcgtcgaa ataaaaggaa taccagttct cctggctgcc cagtaccttc ggattcaccc
     4741 gcgccacgat attgtaatac cagcccggca gcgggttcaa cgaattgttc cgcgtgtcat
     4801 acagcaggtt gagggtaata cccgaagaaa atgagttcgt atgattggca gtcccatacg
     4861 tataccccgt gaatttctga atgtcgatcg tgtcgttatc cggccggata tgaatatgat
     4921 aatccaggtt gtagcccagg ccggcaaaaa gaaagggctt gttggcaatg ctccacaacg
     4981 cattctgata aaaccggaca taggtataac gcaacagtat ctttttgctt ggcgccgtgt
     5041 tcccccccaa tccccacgta aactggggga aaaaggtaag ccggtagtcc ccgctgaaat
     5101 tccacttatt ccccggcgac cagacattcg atcggaaagg gatgttccat tcgcctcgca
     5161 gattggtgct gggagaaaag gtgacgttgg aaagaaaggt ggtcttgggg tcgcccaggt
     5221 taaaggccgc gttcgtcgcg gtgatcagcg cctctccgcc gccgggaacg gaggttccca
     5281 ggggaataaa agtgtaatat acccgttgcc cggccacctt cggctgcttc ttgatgtgga
     5341 tatgggtgaa cttcagcaca accccgatga tatcccgttg ctccgtcgtg tcctctacca
     5401 atttctccgg cgggacaagc gtcccggttg ggttctgggc catcaggcaa aaacaccggc
     5461 agcagcaaca tacgagggca agggcagtga aaactttcgg catagataca agagtaccaa
     5521 tattgtgcta tttttcaggt atgaataatt ccgccttttt acggcccgat ggccctgcgt
     5581 cccggcgtt
//
LOCUS       DATGFG010000804         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1604222, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000804 DATGFG010000000
VERSION     DATGFG010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604222"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..660)
                     /locus_tag="VKQ52_15150"
     CDS             complement(265..660)
                     /locus_tag="VKQ52_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HLZ88590.1"
                     /translation="MQNFRTGFVFDESLNASFLAELFEGDTIYAETVFEDFLRDLPVY
                     WDRVEAAYDNKNLPVLGTSVHTCKTLFGYVGFTDLQELCQQFENKCSVNTVEELRLDY
                     IRLIQRKERAQHIIEKEYNRLRKFNESNG"
     gene            800..1243
                     /locus_tag="VKQ52_15155"
     CDS             800..1243
                     /locus_tag="VKQ52_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88591.1"
                     /translation="MKKFRPLFLLLITVGSVATSTTVYGQAQCNLLQANNAAAVAHAA
                     SANYWFGVMELPFLFISVFFAFLTAYALRGGRFGKGMTFMAWGFLVMAVGHLHMQIEH
                     YYGINIFKTLLGQVSGSVAWFVALIVTWGLSGLGFWSIYKASKNA"
     gene            1270..>3180
                     /locus_tag="VKQ52_15160"
     CDS             1270..>3180
                     /locus_tag="VKQ52_15160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88592.1"
                     /translation="MLKQLLKGRGKQPAARKPGDDSVLYTALFEYLISCVSVPANAEQ
                     LIQLSYKSAGKGAEEVFHTYLLFEKYLTSFEQMEQHTRKSLRERVRQRFPVLLKDGSI
                     FNILFVSETEQKNTLAIHFLQPFLGAVKDKFGRAGDGYFERKVNELAELDTQRRDPEA
                     FRKLQFLSFDVFKFIGNNYGEPLAGKIFEKSYDSFSNKYKELEFFPYMITLVPKEIVD
                     REHLGIFTQAQIEQIFLEKLAESEQLNIALDQKIKENERTQRLLRKNEIMLSGVISSA
                     LDAIVITGAYGHIIHWNQAAVEIFGYTEQEVKGRTLLETILPLSYDHTHWNGFPGFQE
                     QGISGQPGTPMLNQRHELTAVRKSKEEFPIELTVTAIVDENESYYSAFIRDISSRRQR
                     EQELVQMKEKAEQAAKAKSQFLSVMSHELRTPLNAVIGITHLLLQSQPREDQQEDLRT
                     LQFSGESLLHIINDILDFTKLDSGKIELSSIDFNLRELTQSLYQSFSFKAKEKNIVFD
                     VEYDDRMPFYVKGDNFRLSQVLNNLISNAIKFTREGFVKLKVEMVENNGGNFITRFSV
                     IDSGIGIAEDKQEKIFEQFTQADSDTTRLYGGTGLGLSISARLVELMGSSILISSMPG
                     KGSNFNFTVTLQL"
BASE COUNT          777 a          789 c          823 g          791 t
ORIGIN      
        1 tcgacctttt cccgatcgag gaattccagg gcatctgtta cattcaggaa atgccctttt
       61 acctctacga ggtcactctt ctcgcagaac ttacgaagaa aatccgcagc gatctggata
      121 tcttctacta taatacaaga caacttcata gcatccgaaa gcgatccatt caaacaaaaa
      181 caaccctacc cggatgaatc ttttttgggc aggaggtaag cttctactac taataaccag
      241 tataaccagg cctttgcttc taatctagcc gttcgattcg ttaaattttc taagacggtt
      301 atattctttt tctataatgt gttgcgctct ttcttttcgt tgtatcaggc ggatataatc
      361 caaacgtaac tcctctactg tattaacgga acatttattc tcgaattgtt gacagagctc
      421 ctgaagatca gtaaaaccta cataaccgaa caaagttttg caggtatgta cgctggttcc
      481 caaaacggga aggtttttgt tgtcgtaggc cgcttccacc ctatcccaat agaccggcag
      541 atcgcggagg aaatcctcaa atacggtttc tgcgtaaatg gtatcacctt cgaagagctc
      601 cgccaggaag ctggcgttca ggctttcatc gaaaacaaac cctgtacgga aattttgcat
      661 aacggctgca atttttttgt ttgtcgctcg ttaagtaaat aattatatgt acgatgtaca
      721 ggtaatttcc cggtaacggg agaacgagac tctatccaga cgggaaaatt gcgcatttta
      781 ttgtccaaaa tccatattta tgaaaaaatt tcgaccctta ttcctgctac tgatcaccgt
      841 aggatctgtc gcaacctcca ctaccgtata cggtcaagcg cagtgtaacc tgctacaggc
      901 gaataacgcc gccgccgttg ctcacgctgc ctcggcgaat tattggtttg gcgtcatgga
      961 gctccccttt cttttcatct ccgttttttt tgcgttcctg acggcgtatg cgctgcgggg
     1021 cggcagattt ggcaagggga tgacgttcat ggcctggggt tttctcgtga tggccgtcgg
     1081 tcacctgcac atgcagatcg agcattatta cggcatcaat atcttcaaga cattgctggg
     1141 acaagtcagc ggttctgtcg cctggtttgt cgctttgatc gtcacctggg gattatcggg
     1201 cctcggattc tggtctatat acaaggccag caaaaacgcc taagttaatc ccatttcgtt
     1261 tgcattccca tgctgaagca actattgaag ggtaggggca aacagcccgc tgcccgaaaa
     1321 ccgggtgatg actctgtact ttataccgcg ctgttcgagt atctcatcag ctgcgtctcc
     1381 gtacccgcca atgccgaaca actgatccag ttgagttaca aatcggccgg caagggtgcg
     1441 gaggaagtct ttcatactta cctgttattc gagaaatacc tgacctcgtt cgagcagatg
     1501 gagcaacata cccggaagtc gttgcgggag agggtccggc agcgctttcc cgttctgtta
     1561 aaggatggct ccatcttcaa tatccttttt gtctccgaga cggagcagaa gaataccctg
     1621 gccattcatt tcctgcaacc ttttctcggt gccgtaaagg acaaattcgg cagggccggt
     1681 gacggctatt tcgaacggaa ggtgaatgaa ctcgccgaac tggataccca gcgacgggac
     1741 ccggaggcat tccggaaatt gcagttcctg agtttcgatg tctttaagtt catcggcaac
     1801 aactatggcg aaccgctggc ggggaagatc ttcgagaaga gctatgactc cttttccaac
     1861 aagtacaagg aactggagtt ctttccgtat atgatcaccc tggttccgaa ggagatcgtc
     1921 gaccgggagc atcttggcat tttcacccag gcgcagatcg agcagatctt cctggaaaaa
     1981 ctggcggaat cggagcagct gaatattgcg ctcgaccaga agataaagga gaacgagcgg
     2041 acgcagcggt tgctgcgcaa gaacgagatc atgctgagcg gggtgatctc ctcggcgctg
     2101 gatgccatcg tcatcaccgg cgcatacggc catatcatcc attggaacca ggccgcggtg
     2161 gagatctttg gctacacgga acaggaagta aagggacgaa cgctcctgga gacgatcctt
     2221 cccctgtcct acgatcacac ccattggaac ggtttccctg gcttccagga gcagggtatt
     2281 tcgggacagc ccggcacacc gatgttgaac cagcgtcacg agcttacggc cgtgcgcaag
     2341 agcaaggagg agtttcccat cgagctgacg gtgacggcca tcgtggatga gaacgagtct
     2401 tactacagcg cttttatccg ggatatttcc agccgccggc aacgggagca ggaactggtg
     2461 cagatgaagg agaaggccga gcaggcggca aaggcaaaat cgcagttcct cagtgtgatg
     2521 agccatgagc tccgtacgcc gttgaacgcg gtgatcggca ttacgcatct tctgctgcag
     2581 agccagccaa gggaagatca gcaggaggac ctgcgcacgc tgcagttctc gggggagagc
     2641 ctgttgcata tcatcaacga tatcctggat ttcaccaagc tggattcagg gaagatcgag
     2701 ctgtcgtcga ttgatttcaa cctgcgggaa ttgacccaga gcctttacca gtcctttagc
     2761 ttcaaggcga aggaaaagaa tatcgttttt gatgtcgagt acgacgaccg gatgcccttc
     2821 tatgtcaagg gcgataattt ccggctgtcg caggtgctga acaatcttat cagcaatgcc
     2881 ataaaattca cccgggaagg gtttgtcaag ctgaaggtgg agatggtgga gaacaatggc
     2941 ggcaatttta ttacccgctt ctcggtgatc gattcgggga tcgggatcgc cgaggacaag
     3001 caggagaaga tcttcgagca gttcacccag gcggacagcg atacgacgag gttgtatggc
     3061 ggtacggggc ttgggctttc gatctcggcc cggctggtgg agctgatggg cagttccatc
     3121 cttatctcga gtatgcccgg gaagggttcc aatttcaatt tcaccgttac cttgcaactg
//
LOCUS       DATGFG010000805         3809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_863044, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000805 DATGFG010000000
VERSION     DATGFG010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863044"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1067)
                     /locus_tag="VKQ52_15165"
     CDS             complement(159..1067)
                     /locus_tag="VKQ52_15165"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HLZ88593.1"
                     /translation="MAYLKLLRPKDWAKNLFLFIPLFFAGEFLDKAKLISLFFGFIAF
                     SCIASSIYIINDYRDREDDRKHPVKCKRPLASGAVSPAAAITVCALLIILGFGIAWMT
                     RQEFVVVLGIYFLLNLGYCFGLKNIAILDIFILAIGFVLRIKSGSVIADVLLSEWIII
                     MVLLLAIFMAIGKRRDDLLLKISSGSDMRKSIKGYNLELLNVLLALVSSIIIVSYLMY
                     TMQPEVKERLGTYRLYYTCIFVLAGLMRYLQLIYISEDSGSPTRILYKDRFIQVTLIL
                     WILSFATILYFKNVPIFNILRDFYSK"
     gene            1153..3279
                     /gene="recG"
                     /locus_tag="VKQ52_15170"
     CDS             1153..3279
                     /gene="recG"
                     /locus_tag="VKQ52_15170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HLZ88594.1"
                     /translation="MASILSNPIEYLKGVGPLRAELLQKELGIFTFQDLLEHFPYRHL
                     DRTKINLIRDIDVSTEYIQVAGKVIGVELLGDKRSRRLVAELRDASGTLELVWFQGIH
                     GVQKFVQVGGEFLVYGRVSFFQGSPQLTHPEIEVLQGQGAAPPEFLEPVYPTTEKLKV
                     RGLGGRQLGKLTQVLLNMLKEKDLPENLPASLLSYPDADTGVPKVWLSRYEAYRQVHF
                     PTKPALFHQAVNRLKFEELFLAQLRLGLIKSKRHRSSHGVVFTQVGELFNRFYTHHLP
                     FELTGAQKRVLKEIRRDTGSGRQMNRLLQGDVGSGKTMVALLSMLLAIDNGFQACLMA
                     PTEILARQHYQGISALLKDLPLEVRLLTGSSRSKSRKEVLGLASEGKLHILIGTHAVL
                     EEAVQFRRLGLAIVDEQHRFGVAQRARLWSKAEIPPHVLVMTATPIPRTLAMTAYGDL
                     DYSVMDELPPGRQPISTVHRYEDRRPQVMDFIRHEIGKGRQAYVIFPLIEESETLDYE
                     NLERGYENIKAWFPEPKYWISRVDGRQKQEIRDTNMQRFLSGDTQIMVATTVIEVGVN
                     VPNATVMVIESAEKFGLSQLHQLRGRVGRGTEKSFCILLTSPRLGKEARERMSILTET
                     NNGFKIAEKDLEIRGPGDIEGTRQSGLLNFKLANIVQDRYWLELARDRAMELLEKDPD
                     LEMEENKALKAFLQSQRGKTAWSKIS"
     gene            3295..>3809
                     /locus_tag="VKQ52_15175"
     CDS             3295..>3809
                     /locus_tag="VKQ52_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88595.1"
                     /translation="MRITLLSTLFFLSFLRLSAQSGGYSNLEFIENKGQWDTLIRFNA
                     AIGTGNLFMQRKGFTVLLHDTNDMKRIGLLLHGDVGAAGVGSPGGGTGKSAASVAGNK
                     SVAAQQRQAVSSAPGNYHYPGNPNGGGSTGSGSGSDPFLLHSHAYRVSFENANDGVQI
                     VPDKALDSYNN"
BASE COUNT          899 a          823 c         1242 g          845 t
ORIGIN      
        1 atcccgaagc ggatggagag atcggagcgg aggggttcca gtcgctgggg gtttctggcc
       61 gccagttgta catcgtggcc tgctgaagcg aatttccggg ccagggcggt agctatatcc
      121 gaacctgcac cgagtaataa aagggagggc atgaaaggtt atttagaata gaagtccctt
      181 aaaatgttaa atataggaac attcttaaaa tacaggatgg ttgcaaagct taggatccat
      241 aggataaggg tgacctggat gaaccggtct ttgtagagga tccttgtggg ggagccggaa
      301 tcttcggaaa tgtagatcaa ctgcaggtag cgcatgaggc ccgccaggac gaatatacag
      361 gtgtagtaga gccggtaggt gccgagtcgt tcttttactt cgggctgcat ggtatacatc
      421 aggtaggaga cgatgatgat ggaggagaca agggccagca gtacgttgag caattcgagg
      481 ttatagcctt ttatggattt gcgcatgtcg gagccggagc tgatcttcag caacaggtca
      541 tcccggcgtt tgccgatcgc catgaagatc gccaggagca ggaccatgat aatgatccat
      601 tcggagagga gtacatcggc gataacggac ccggatttga tgcggagcac gaagccgatg
      661 gcgaggatga agatatccag gatggcgata ttcttgaggc cgaagcaata gccgaggttg
      721 aggaggaagt agatgccgag gacgaccacg aattcctggc gggtcatcca ggcgatgccg
      781 aagccgagga taatgagcag ggcgcagacg gttatcgcgg cggcgggtga aacggcgccg
      841 gaggccaggg ggcgtttgca ttttacagga tgtttccggt cgtcttccct gtcgcggtaa
      901 tcatttatga tgtagatact gctggcgatg caggagaagg cgataaaccc gaaaaagagg
      961 cttatcagtt ttgccttatc gagaaactca cctgcaaaaa agagaggaat aaacaagaac
     1021 aggttctttg cccagtcttt cggtcttaac aatttaagat acgccaaaac agtattttgt
     1081 cgcgacaaaa tacaaacaaa acgacaatag ttataactta tttttgtgta aatttccttc
     1141 ttcatcaata ctttggcatc catactttcg aatccgatag aatatctgaa aggcgtaggc
     1201 ccgttgcggg ccgagctatt gcaaaaggaa ttgggtattt ttactttcca ggacctgttg
     1261 gaacattttc cctatcgtca tctggatcgt acgaagatca atctcatcag ggatatcgat
     1321 gtttcgaccg agtatatcca ggtagcgggt aaggtgatcg gtgtggagtt gcttggtgac
     1381 aagcggagcc ggcggctggt ggccgagtta cgggatgcat cgggtacact ggagctggtt
     1441 tggtttcagg gtatccatgg ggtgcagaag tttgtccagg tgggtgggga atttcttgtg
     1501 tatggccggg tgagtttttt ccaggggagt ccgcagctga cgcatccgga gatcgaggtt
     1561 ttgcaggggc agggggcggc gccgccggag ttcctggagc cggtgtatcc gacgacggag
     1621 aaactaaagg tccgggggct tggggggcgg cagcttggga agctgaccca ggtgcttctt
     1681 aatatgttaa aagagaagga cttgccggaa aacttgccgg cttccctgct atcctaccca
     1741 gatgccgata cgggtgttcc gaaggtctgg ctttcgcggt atgaggcgta caggcaggtg
     1801 cattttccga cgaagccggc gttgttccac caggccgtga accggctcaa gttcgaggaa
     1861 ttgttcctgg cgcaactgcg gctggggttg atcaagtcga agcggcaccg gtcctcgcat
     1921 ggggttgtct ttacgcaggt gggggagctc ttcaaccggt tttataccca tcacctgcct
     1981 tttgagctca ccggcgccca aaagcgggta ttgaaggaga tcaggcggga tacgggcagc
     2041 ggccggcaga tgaaccggtt gttgcagggg gatgtcggga gtgggaagac gatggtggcg
     2101 ttgctgtcga tgttgctggc gatcgacaac ggctttcagg cctgtctgat ggcgccaacg
     2161 gagatactgg cgcgtcagca ttaccagggc atatcggctt tgctgaagga tctgccgctg
     2221 gaagtccggt tattgacagg atcatcccgg tcgaaaagcc ggaaggaggt gctgggcctc
     2281 gccagcgagg ggaagttgca tattcttatc gggacacatg cggtgctgga agaagcggtg
     2341 cagttccggc ggctggggtt ggcgatcgtg gacgagcagc accggtttgg cgtcgcgcag
     2401 cgtgcccggc tgtggagtaa agcggaaatt ccgccccatg tgctggtgat gacggcgacc
     2461 ccgattccgc ggaccctggc gatgacggca tatggtgatc tggactatag tgttatggac
     2521 gaattacccc ccggaaggca accaatctcg actgttcatc gttacgaaga caggaggccg
     2581 caggtgatgg atttcatccg gcatgagatc ggaaaaggac ggcaggcgta tgttattttc
     2641 ccgttgatag aggagtcgga gaccctggat tatgagaacc tggagcgggg gtatgagaat
     2701 ataaaggcct ggttccccga accgaaatat tggatcagcc gggtcgacgg gcggcagaaa
     2761 caggagataa gggatacgaa tatgcagcgc tttctaagcg gggatacgca gatcatggtt
     2821 gccaccacgg tgatagaggt cggggtcaat gtgccgaatg cgacggtaat ggtcatcgag
     2881 agtgcggaga aattcgggtt gtcccagctg catcaattgc gggggagagt aggacgtggc
     2941 acggagaaga gcttttgcat cttattaacg agtccccgtt tgggaaagga ggcgcgggag
     3001 cggatgagta tccttaccga gacgaacaat ggcttcaaaa ttgctgaaaa ggacctcgaa
     3061 atccgggggc ctggcgatat cgaggggacc cggcagagcg ggttattgaa ctttaagctt
     3121 gcaaatattg ttcaggacag gtattggctg gagcttgccc gtgaccgggc gatggaacta
     3181 ctggaaaagg accccgacct ggagatggaa gaaaataagg cgctgaaggc tttcctgcaa
     3241 tcgcagcgcg gcaaaacggc gtggagcaag atatcgtaat taatataaag agatgtgaga
     3301 ataaccctac ttagcacgct attcttctta tcgttccttc gcctttctgc acaatccggg
     3361 ggctattcca atcttgaatt tattgagaac aagggccagt gggacacgtt gatccggttc
     3421 aatgcggcga tcggcacggg gaacctgttc atgcagcgta agggttttac ggtgctcctg
     3481 cacgatacga acgatatgaa gcggatcggt ttgctgctgc atggggatgt aggtgctgcg
     3541 ggggtgggga gcccgggcgg tgggacgggt aaatcggcgg cgagtgtcgc gggcaataag
     3601 tcggtggccg cacagcagcg gcaggcggtg agttcggcgc cggggaatta ccattatccg
     3661 ggcaatccca atgggggtgg ctcgacgggc agcggatcgg ggagtgatcc gttcctcttg
     3721 cattcgcatg cctaccgggt gagtttcgaa aatgcgaacg acggcgtgca gatcgttccg
     3781 gacaaggcgc ttgattccta taacaacta
//
LOCUS       DATGFG010000806         6874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_380337, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000806 DATGFG010000000
VERSION     DATGFG010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380337"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..4217)
                     /locus_tag="VKQ52_15180"
     CDS             complement(48..4217)
                     /locus_tag="VKQ52_15180"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HLZ88596.1"
                     /translation="MKAIQTRTNAAQPAKKTAPFFKKGAGQGFFKPAATGVQTKLTVG
                     EPDDAYEKEADATADRVVKGLHSEPAGGGSGGSGFGGGRGGPGGGGGAGKGISRKPIF
                     ESENDPADAVQRKCAACEQEEKVQKKGAGGAATGGAMAASPAVESGISRSKGSGSSLP
                     TATRSQMESSIGADFSGVRVHQDSGAQQMNKDLNAQAFTHGKDIYFDSGKYNPGSSEG
                     KHLLAHELTHVVQQTGAGSPKKTAQRKAKSAGGAAQAGGTSGALSAGSAASVSGLAGG
                     RSTGSMDAASKASGVDAGMTESPLEGMREEGPETMGVEAVQRTPVGQPAGIQRLAGTI
                     QRDGASGQTKNQQDLANVTAPPEGKIETSKTLVLTNFPLKTYSGVKAPAMPSKRVRKP
                     IPGERATAQGGKWKTKVYDNVMKRLQDVGDNPKPTDLFMLTIKDKGGKGSQAGKPGAG
                     VKGDSGKAQIIGDIPTLADAVKVPYWNRSGKPTLHQIEHILDWQIVGNDADDIDNNLI
                     LVDKQTNQKQNIRVRDLIKQKIGAVQTAYKTAKIDMPIVNEAVTDTYYKKFRIFFENF
                     QPPAADQSPEESGSIVQPADMEKNDDIVSKTTIELNHPCIPEGYCVVATAIGGEGFIV
                     PYSGENEAIRFIGDKKAKMLNGMEQKNIIKAGATDVMKNSKDQPLKLAFEKDDKIPNV
                     GGYYKLLNTGHATELKEILKLRGLSPIEFDPQVEVSPETGITAKGKVISEIPILKDAD
                     ISVELSGGEFTVSGTLSSDMLKGKLPSPLDVESCDLTLSASTGKGWSVGGSIDFTIKN
                     IGKGSLSASIDGEKFSLNGDFTFDETKYYKNAKLSIGYVRMKDTGEGKWSGSGGLDFA
                     ENAIPGIKSGNITLGYAEGAISGKGHFLTTIPKLKQVDVSASFDAKGNFSVTGDTSLE
                     GVPGISENHITATLTRDESGYSLAILGTATPNLPNIPGLGATLTVSYNKGVFMLEGTA
                     TYEKDKLKGSLTVGVTNAVVDDNGVASKDATDEKIKIYGNGSITLHLLKGVDATLQGA
                     VDGNGEMFIWGEMDVTATPFDPIDIDKTLFDVKTSIPLVGIPFASINLDLGTSGKFYF
                     HWDPLTIKLKATLDRTNIKNIGEAGGELTANLSSHAKTGFVFTISAGVSVSIAIIKLG
                     AHLNGNVDLGLNAEAGVDAKAHWDMQKGLTLEAAEAHLTGVPFVTFSLSGDITADVDL
                     WLTSYNVYKKEMTFAQKTLDLSQFAFGVSVPLKITEDGKIEGIDYSKIKVQPEMGKDA
                     GDNISDQVLNGDDRKIKEQKEKEAREKIRTKVQQKLQAKKADGDTDFAAYADDLKSDI
                     KDDTPDELASMVDQIVDEEVQKMIKQQDAEQAAARAAMAAPGSGVPAFLGGPPDDDSP
                     KPNPVNQATKT"
     gene            complement(4218..5987)
                     /locus_tag="VKQ52_15185"
     CDS             complement(4218..5987)
                     /locus_tag="VKQ52_15185"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HLZ88597.1"
                     /translation="MAQLFEKAVRVIPGGSKEIVMQHLIKKQELLLRVAPGPDPFGVQ
                     HAASGYYRDRLLPVLERVFDELSVEEEVILIDRLVIDLGRIAEKELRGGAPEDDLYAV
                     IRKEVLRVIQGERPDAGLSRAALRENVLSQWWYYMEKGRLPWNVTVTTPGWYRSVLEL
                     LSVDYDRVTRLRRMLEGSAVFLQRVSYQHPGEFLETLTGILAGARQPLLGQRVEDLCF
                     IFRWLEKRYHEMTDITVTDHGRAREERAVVAEALHRWKQGHAAFLALPEPGRKAAVWQ
                     ALLREAAMRPVELKADGAEKVVLRRFLMPGEVVIRKLLQEAGLDLPGLWIGGDQRDGD
                     RGDDGDRGDGGNRDDDRVAPAKERDVADESMADRTAGRVEKKVEERDVSAAPPGDSAA
                     SAPGDSAASAPGGSAVDEDAAAASSGEVVDEEGLYGRYAGLVLVHPFLSTFFTRCGLW
                     DGGGFVSLAARQQAVFLLHYLATGEKEGPEYDLLFPKLFCGYALEAPLPGRIEIPESA
                     FGEGSALLENVLLRWEKLKNTSVTGLQEGFLQRTGKLINRNGKMVLLMEMSAIDVLLD
                     YLPWGLGIVKLPWLKELLFVEWR"
     gene            complement(5954..>6874)
                     /locus_tag="VKQ52_15190"
     CDS             complement(5954..>6874)
                     /locus_tag="VKQ52_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88598.1"
                     /translation="YAYAEMASRAMERAKDCINMEGMHLVEHLLLRPEKGGTKAIPIC
                     ETSGNTGLPFQPGADGYSFIATAFLPAWPERFRKKENRQVLETLLQEETPAHILLRIL
                     WLTPEDMCRREGMYKDWLRWLIPEKPCGDFKMEEWVRLLFEESPRCLANCAACTVASE
                     TAGSGSAIARSVDGDEWLSEINLLYCWKDMACADTSKWKFDTPVTNPKPGGIQFTVTP
                     KPVVTVKKPANTLVGKQGPGNNPRTRPVKADDPPRLAADRVKVAADKVPHPSGIQAEA
                     ERIVVDEPPVRPGAIPETSWWRSFLRRLFG"
BASE COUNT         1278 a         2366 c         1635 g         1595 t
ORIGIN      
        1 gtttcgatgc cggtaattcc gtaaatgata gctcataaaa aaataattta ggtttttgtc
       61 gcctggttca ccgggttcgg cttcggcgaa tcgtcatccg gcggtccgcc cagaaaggcg
      121 ggaaccccgc ttcccggcgc agccatcgcg gccctggcag ccgcctgttc cgcatcctgc
      181 tgcttgatca ttttttgtac ctcttcgtcc acgatctggt cgaccatgct ggccagttca
      241 tccggcgtat catccttgat atcgctcttc agatcatccg cgtaggccgc gaaatcggtg
      301 tccccatccg cttttttcgc ctggagcttc tgctgcacct tggtcctgat cttctcccta
      361 gcctcctttt ccttctgttc ctttatcttc cggtcatcac cgttcaacac ctggtcggag
      421 atattgtcgc cggcatcctt gcccatttcc ggctgtactt ttatcttgct gtaatcgatg
      481 ccctcgatct ttccatcctc ggtgatcttc aaggggaccg agaccccgaa ggcaaactgg
      541 ctcaggtcga gggtcttttg agcaaaggtc atttccttct tgtagacatt atacgatgtg
      601 agccagagat cgacatcggc ggtgatatct ccgctcaggg agaaggtcac gaacggaacg
      661 ccggtgagat gggcctctgc tgcctccagg gtcaacccct tttgcatatc ccagtgcgcc
      721 ttagcgtcca ctcctgcttc ggcgttcagc cccaggtcta cattcccgtt gaggtgggcg
      781 ccaagtttga tgatggcgat actgacactc acccccgcgg aaatcgtgaa cacaaatcct
      841 gtcttggcat gggagctcag attggctgtc agttcgccgc cggcttcccc gatattcttg
      901 atattcgtcc ggtcgagcgt cgcctttaat tttatcgtca gcggatccca gtggaaatag
      961 aactttccac tcgtacccag gtcgagattg atactggcga atgggatccc taccagcggg
     1021 atcgacgtct tgacatcgaa cagggtcttg tcgatatcga tcgggtcgaa cggggtagcc
     1081 gtcacatcca tctcacccca gataaacatc tcgccattgc catccaccgc gccctgcagc
     1141 gtagcgtcga caccctttaa cagatgcagg gtaatggagc cattgccata gatcttgatc
     1201 ttctcatccg tcgcatcttt cgaggccacc ccattgtcat ccaccaccgc attggtcaca
     1261 cccaccgtca gcgaaccctt cagcttgtcc ttctcatagg tggccgtacc ctcgagcatg
     1321 aagacgccct tgttgtagct taccgtcagc gtcgcgccaa gacccgggat attcggcaga
     1381 ttgggcgtcg ccgtcccgag gatcgcaagc gaatagccgc tctcatcccg ggtaagtgta
     1441 gccgtgatgt ggttctcgct gatgcctggc acgccttcca ggctggtatc cccggtgaca
     1501 ctgaaattgc ccttggcatc gaaggaggcc gacacatcca cctgtttcaa tttcgggatg
     1561 gtcgtcagga aatgcccctt cccgctgatg gccccttccg catacccaag ggtgatattc
     1621 cccgatttga tgcccgggat ggcattctcg gcaaaatcaa gcccacccga tccgctccat
     1681 ttcccttcac cggtatcctt cattcgtaca taaccgatcg acagcttggc attcttatag
     1741 tatttggttt cgtcaaaggt gaagtccccg ttgagcgaga atttctcacc gtcgatggaa
     1801 gcgcttaggg agcccttccc gatattcttg atggtaaaat cgatcgatcc gccaaccgac
     1861 catcccttac ccgtactggc cgagagagta agatcgcagc tctcgacatc cagcggtgaa
     1921 gggagcttgc ccttcagcat atccgaggac agcgtcccgg acacggtgaa ctccccgccg
     1981 gacagttcga cggagatatc cgcatctttc aggatcggga tctcggatat tacctttcct
     2041 ttggccgtga tccccgtttc gggacttacc tccacctgcg gatcgaattc tatcgggctc
     2101 agcccacgca atttcaggat ctccttcaat tcggtcgcat gccccgtatt cagtaattta
     2161 taataaccgc ccacattggg gatcttgtca tccttttcaa aggccagctt cagcggttga
     2221 tccttgctgt tcttcataac gtcagtggcc ccggccttga tgatgttctt ttgctccatt
     2281 ccattcaaca tcttcgcctt cttgtccccg atgaaccgga tcgcctcgtt ctcgccggag
     2341 taggggacga taaaaccctc gccgccgatg gcggtggcca cgacacaata tccttcagga
     2401 atgcagggat gatttaattc gatggtggtc ttggagacga tgtcatcatt cttctccatg
     2461 tcggccggct ggacgatcga acccgactct tccggtgact ggtcggcggc aggcggctgg
     2521 aagttctcga agaaaatgcg gaatttctta tagtaagtat cggttacggc ctcgttgacg
     2581 atcggcatat cgatctttgc ggttttgtat gcggtctgca ccgcaccgat cttttgtttt
     2641 atcaggtctc ttacccttat attttgtttc tggttggtct gcttgtccac gaggatcagg
     2701 ttattgtcga tgtcgtcggc gtcgttcccg acgatctgcc agtctaagat atgctctatc
     2761 tggtggaggg ttggcttgcc cgaccggttc cagtagggaa cctttactgc gtccgccagc
     2821 gtggggatat ccccgatgat ctgtgccttg ccactgtccc ccttgacccc ggcgcccggc
     2881 tttccggcct gggatccttt tccacccttg tccttgatcg ttaacatgaa gagatccgtc
     2941 ggcttcggat tgtcccctac atcctgtaat cttttcatga cattgtcata caccttggtc
     3001 ttccatttgc cgccctgggc agtcgcccgc tcgccgggta ttggttttcg tacccgcttc
     3061 gaaggcatcg caggtgcctt gacacccgag taggtcttca acgggaaatt ggtgaggaca
     3121 agcgttttgc tggtctcgat cttaccttcc ggcggcgccg tcacattggc aaggtcctgc
     3181 tggttcttgg tctggccgct ggccccgtcc cgttgtatcg tccccgcgag ccgttggatt
     3241 cctgccggct gacccaccgg cgtccgctgt acggcttcaa ctcccatcgt ctccggccct
     3301 tcctcccgca tgccctccaa cggcgactcc gtcattcccg catccacccc actcgccttc
     3361 gacgccgcat ccatgctccc cgtcgatcgc ccgccggcta atccgctcac actggccgcc
     3421 gagcccgcac tcagcgcgcc gctggtccca cccgcctgag ccgccccgcc cgcgctcttc
     3481 gccttccgct gcgccgtttt cttcggactt cccgcacccg tctgctgtac tacatgcgtc
     3541 agctcatgcg cgagcaaatg cttgccctcg ctgctacccg gattatactt gcccgaatcg
     3601 aaatagatat ccttcccgtg tgtaaaggcc tgcgcgttca ggtccttgtt catctgctgc
     3661 gcaccgctgt cctggtggac ccgcacgccg gagaaatcgg cgccgatcga agactccatc
     3721 tggctgcgtg tagccgtggg taacgacgaa ccggaaccct tcgatcgcga gatgccgctc
     3781 tccactgccg gactggccgc catggcgccc ccggtagccg ctccgcccgc tcctttcttc
     3841 tgcacttttt cctcttgctc acaggcggca cacttccgct gcacagcatc ggccggatcg
     3901 ttctcactct cgaagatcgg cttacggctg atccccttgc cggcgccccc gccgccgcca
     3961 gggccaccgc ggccaccacc aaaaccactg ccacccgaac cgccgcctgc cggctccgaa
     4021 tgtaatccct tcaccacccg atcggccgtc gcatccgcct ccttctcata ggcatcatcc
     4081 ggctcaccca ccgtcaattt ggtctggaca cccgtcgctg caggcttgaa aaatccctgt
     4141 cctgcccctt tcttaaagaa aggcgcggtc ttctttgccg gttgagcggc gttcgtcctg
     4201 gtctggatcg ctttcattta tctccattca acaaataata attcttttag ccagggcagc
     4261 ttcacgatgc ccaaccccca gggcaaataa tccagcaaca catcgatggc actcatctcc
     4321 atcaacagga ccatcttccc gttgcggttg atcagttttc cggttcgctg caggaaacct
     4381 tcctgcaacc ccgtaaccga cgtattcttc agcttctccc agcgcagcag cacattctcc
     4441 aacaacgccg acccctcccc gaaggcggat tcggggatct cgatcctccc cggtaacggc
     4501 gcctccaggg catagccgca aaaaagcttc gggaagagca gatcgtattc gggaccctct
     4561 ttctctcccg tcgcgagata gtgcaacagg aacaccgcct gctgccgcgc cgcaagactg
     4621 acaaagccgc ccccatccca tagcccgcaa cgggtaaaaa aggtcgaaag aaagggatgt
     4681 accaggacca accccgcgta ccggccatac aacccttcct catccacgac ctcgcccgaa
     4741 gacgccgctg ctgcatcttc atctacggcc gaaccgccag gggccgaagc cgccgaatcg
     4801 ccgggcgcag aagccgccga atcgccgggg ggcgccgccg acacatcgcg ctcttccact
     4861 ttcttctcta cgcgacccgc cgtcctgtcg gccatgcttt catcagccac atcgcgttcc
     4921 ttcgccggcg caaccctgtc atcatcgcgg ttcccgccat ccccccggtc cccatcatcc
     4981 ccgcgatcac catcccgctg gtctccgcca atccacaacc ccggcagatc gaggcctgcc
     5041 tcctgcaaca acttccggat aactacttcc cccggcatca aaaaacgccg caggaccacc
     5101 ttctccgcac catccgcctt cagctccacc ggccgcatcg ccgcctcccg gagcagcgcc
     5161 tgccacaccg ccgccttccg ccccggctcc ggcaacgcaa gaaaagccgc atgcccctgc
     5221 ttccaccggt gaagcgcttc cgccacgaca gcccgctctt cccttgccct gccgtggtcc
     5281 gtcaccgtta tatccgtcat ttcatgatat cgtttctcca gccagcggaa gataaaacaa
     5341 aggtcttcca cccgctgccc cagcaagggc tgccgggccc ccgccaggat acccgtcagc
     5401 gtttccagga actcccccgg atgctgatag ctcacgcgct gtaaaaagac cgcggacccc
     5461 tcgagcatcc tgcgcaaccg cgtgacacgg tcgtaatcca ccgacagcag ttcgagcacc
     5521 gaccggtacc agccgggtgt ggtcaccgtc acattccagg gcagccgtcc cttctccatg
     5581 taataccacc actggctcaa tacattttcc cgtaaggcgg cgcgcgagag acccgcatca
     5641 ggcctctccc cttggataac cctaagcact tccttcctta tcaccgcata aaggtcatcc
     5701 tccggcgcgc cgcctcgcag ctccttctcc gcaatccggc cgagatcgat caccagccgg
     5761 tcgatcagga taacttcttc ctccaccgac aattcatcaa acacccgttc caggaccggc
     5821 agcagccggt cacgataata accgctggcc gcgtgctgca cgccaaatgg atcgggaccc
     5881 ggcgcaaccc gcaacaacaa ttcctgtttt tttataaggt gttgcataac tatctcctta
     5941 ctgccgcccg ggatcacccg aacagccttc tcaaaaagct gcgccaccag ctcgtttccg
     6001 gtatggctcc tggcctgacg ggcggctcgt caaccacgat cctctccgcc tccgcttgta
     6061 tacccgatgg atgcggcacc ttgtccgccg ccacctttac cctgtcggcg gccagcctcg
     6121 gcggatcatc cgccttcacc ggcctggtcc ttggattgtt cccgggcccc tgcttaccca
     6181 ccagcgtatt ggccggcttc tttaccgtca ccaccggctt tggggtcacc gtgaactgta
     6241 tcccacccgg cttcgggttc gtcaccggcg tatcgaactt ccatttggaa gtatccgcac
     6301 aagccatatc cttccagcaa tacaaaagat tgatctcgct cagccattca tccccatcca
     6361 ccgacctggc aattgcactc cctgatccgg ccgtctcact cgcaacagta caggcagcgc
     6421 aattcgcgag gcaccggggc gattcctcaa ataacagccg tacccattct tccattttaa
     6481 aatccccgca gggcttctcc gggataagcc accgcagcca gtccttatac atgccctccc
     6541 gccggcacat gtcctcgggc gttagccaaa ggatgcgcaa caggatatgc gcgggcgtct
     6601 cctcctgtaa aagcgtctcg agcacctgcc ggttctcctt cttccggaac cgctccggcc
     6661 acgccggcaa aaaagccgtt gcaataaagg aatacccgtc ggcccccggt tggaacggca
     6721 gtcccgtatt cccgctcgtt tcacagatgg gtatcgcttt tgtccctcct ttctccgggc
     6781 gcagcaataa atgctcgacc aggtgcatcc cctccatatt gatacagtcc ttcgcacgct
     6841 ccatcgcccg cgacgccatc tcggcatagg cata
//
LOCUS       DATGFG010000807         6476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_290561, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000807 DATGFG010000000
VERSION     DATGFG010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290561"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3002
                     /locus_tag="VKQ52_15195"
     CDS             <1..3002
                     /locus_tag="VKQ52_15195"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HLZ88599.1"
                     /translation="SAARLSFFRHFPALTLTLYDVKLNGSAPFAKERLIEADEIALGV
                     DLRSVFSEINIDKIFLTNAFINIQVDTAGHANYNIYAAKKSNPSAPADSGSASLKIQK
                     IIIERSRLTYNDASLPILINARGLEYTGNGDLSQAIFDLHTHMQLESIDFYYNRVPYF
                     INKKLDADLITKINTNSLALIFERNELLINQLPVAFTGEFSFLKDGYDMDFRLNASNA
                     RLHDIFTALPPDMLAWLDSTDVKGVGDIDAALKGRYVAATNTMPDLLFDLRVRDGYIA
                     YAKAPAPVSDLRLDFHSRLPGLDPDSLSVTVDSAFFHLGKDYFNADLKLKGLKTPWVA
                     ANIQSEMDLEKWDQALGFAPADLKGRYTLQLKAEGRYATRVGRTTTLRRTKLDTVVTS
                     IPHFTLTSTLRNGFCKLPSRPEAVSGISFDLDASCPDNDYHHTRLTVDNFNARVLTSY
                     LKGFFHLDNATNFSIEAGLETVFHLADLKKVYPLDSMDLAGDLSAHVRTKGNYLPAKH
                     QFPVTEADLRLDNGRIQTKYYPHPLEQIQVGARLTSKGGSLKDLDLAITPVSFLFEGQ
                     PFVLKADLQNFTDLNYSLTSSGTLDLGRIQKLFAVKDYTITGLVDTRLSLRGRQSDAT
                     SGHYDRLFNEGSLKVKDLQVRSELFPLPFYIYSGDFHFHQDKMWFDSFNASYGRSRFT
                     LNGWLSDVIGYMSNKGQPLNGNFTLNSDLLFVDEFMAFADRTPTATTSATGTSATSTT
                     STGTAPATSGVVMVPADLSVGFHADIKRIQYNGLNIDSFKGGVHLDSGALHLDTTTFT
                     LAGAPVQMNASYTSPDPHRAFFDYHLIAKDFDVQRAYREVKLFHDLASSAAKAQGIIS
                     LDYKLGGRLDDNMRPVYPSLKGGGTLSIRKVKVKGLKLFGEVSKETNKDVNDPDLSKV
                     DIKSTINNNLITIERTRMKVSVFKLRIEGQASFDGRLNLHCRVGLPPFGIIGIPVSVT
                     GTQDNPKVKARRETDKDELEGTEDTAQ"
     gene            complement(2996..4135)
                     /locus_tag="VKQ52_15200"
     CDS             complement(2996..4135)
                     /locus_tag="VKQ52_15200"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HLZ88600.1"
                     /translation="MKPAIFFACLFLFAAFCRPADHSPGQPAGDHPTLAIGAAAPDFS
                     LPGVDGKTYALSSFANARVLVVVFTCNHCPTAQAYEDRLIRLTKDYRDRGVAVVAIMP
                     NDPQSIRLDELGYTDMGDSFEEMKRRASEKKFNFPYLYDGQTEAVSLKYGPIATPHVF
                     VFDKDRKLRYSGRVDDVEKPTKTPHTLDTRNAIEALLNNKEVPVTTTKVFGCSIKWAE
                     KSDWIEKAKAEWAKEPVKLDTISADGIRELIRNKTEKLRVIDVWATWCGPCVAEFSDF
                     VDINRMYRRRDFEFISISADEPAKRDKALQFLKGVQASATNYIFNVDDKYKLIEAIDP
                     KWQGALPYTMVIEPGGKVIYAKDGPIDAAALKKIIVDDPAIGRYY"
     gene            4281..5189
                     /locus_tag="VKQ52_15205"
     CDS             4281..5189
                     /locus_tag="VKQ52_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLZ88601.1"
                     /translation="MHTRRSFLKTSALYSAGILAAPALPSFLAAAGPAIGLQLYTVRD
                     AMAADPAGTLAKVAQIGYNSLEGATYNNGVEKFYGMDPKTFAGELKNNGLVMRSCHYR
                     LGEDPQGATLTNGVYNGTILHDWDKAVEDAHTLGLQYMVCAWLSPKERLGLDHYKKMA
                     NDFNIAGEKCSKAGIQFCYHNHDFEFDAQNGKYPYDVLLESTDPKSVKMEMDLYWVTK
                     AGQDPVALFNAHPGRFPLWHLKDMDSTPQHAFTEVGNGTIDFKKIFASKSTSGMKYFF
                     VEQDRCPGSPFDSITKSITYIKQNLV"
     gene            complement(5170..>6476)
                     /locus_tag="VKQ52_15210"
     CDS             complement(5170..>6476)
                     /locus_tag="VKQ52_15210"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HLZ88602.1"
                     /translation="GFFGERNIRALKEDGAGNIWFIEDNSLGVIDFSGSQPEIIFFPE
                     LNGKLVADYEHIDPYNKYNVFVGAEKGFYHINYEEYKKNHYPIQVRIRSVRAFGKTDS
                     LLYGGYYGEVDDSLEQPSSAIYSISNKWNSLHFEYTSTLYAAQSSIQYSYCLKGFDRD
                     WSEWSKKTEKEYTNLPAGVYTFQVRSKSNLRNESAISTYTFTILPPWYQTGWAYGLYV
                     LLLAVLLCLLYFWQRRIFRRQQKKHEEEQARLQYLHQLEMEKSEKEIVKLKNEKLEAE
                     IEHKNTELASAAMHLVQKGELLGNIREELLRMKKGGNGEGVAADEFKKMLRILGEENR
                     MDKDWEQFAIHFDKVHSDFLRILKTAYPALSAHELKLCAYLRMNLSSKEIAQLENISV
                     RGVEISRYRLRKKLKISTETNLFDFLMELHARGKDSITPNSV"
BASE COUNT         1502 a         2075 c         1514 g         1385 t
ORIGIN      
        1 tctccgctgc ccggctttcc tttttccggc attttcctgc gctcacactc accctttatg
       61 atgtgaagct caacggctcc gcgccctttg caaaagaacg cctcatcgaa gccgatgaga
      121 tcgccctcgg cgtcgatctg cgcagtgtct tcagcgagat caatatcgat aagatctttc
      181 tcaccaacgc ctttatcaat atccaggtcg atacggccgg ccatgccaac tacaatatct
      241 atgcggccaa aaaaagcaac ccgtccgcac ccgccgactc cggcagcgcc tcccttaaga
      301 tccagaagat catcatcgaa cgaagccggc tcacctacaa cgacgcctcc ctcccgatcc
      361 tcatcaacgc ccgcggactc gaatacacag gcaacggtga tctgagccag gccatcttcg
      421 acctccatac ccatatgcag ctggagtcca tcgactttta ttataaccgc gttccctatt
      481 tcatcaacaa gaaactcgac gccgacctca tcaccaagat caacaccaat tccctcgccc
      541 tcatcttcga acggaacgaa ctcctcatca accagctgcc cgtcgccttc accggggagt
      601 tctccttcct caaagatggg tatgatatgg acttccggct caatgccagc aatgccagac
      661 tccacgatat ctttaccgcc ctgcccccgg atatgctggc ctggctggat agcacggatg
      721 taaaaggggt cggcgatatc gacgccgccc tgaaaggaag atatgtcgct gcaaccaata
      781 ccatgcccga tcttcttttc gacctcaggg tccgcgacgg gtatatcgcc tacgcaaaag
      841 cccccgcccc ggtgagcgac ctccggctcg atttccattc ccggctgccc ggcctcgatc
      901 ccgatagcct cagcgtcacc gtggactccg ccttcttcca tctcggcaaa gactatttca
      961 acgccgatct gaaactaaaa ggactaaaaa ctccctgggt ggccgcaaac atacagtcgg
     1021 agatggacct ggagaaatgg gaccaggccc ttggctttgc tcccgccgac ctgaaaggcc
     1081 gctacaccct gcagttaaaa gccgagggac gatacgccac ccgcgtaggg cgcaccacca
     1141 ccctccgccg gacaaaactg gataccgtcg tcaccagcat cccgcatttc accctgacct
     1201 ccaccctccg caacggcttc tgtaaacttc cttcccggcc cgaagccgtc agcgggatca
     1261 gcttcgacct ggacgcttcc tgccccgaca acgactacca ccatacccgg ctgaccgtcg
     1321 ataatttcaa tgcccgggta ttgaccagct atctgaaagg cttcttccac ctcgacaacg
     1381 ccacgaattt ttccatcgaa gccgggctgg agaccgtatt tcatctcgcc gatctcaaaa
     1441 aggtctaccc gctcgacagc atggacctcg ccggtgacct cagcgcacat gtccggacaa
     1501 aggggaacta cctccccgca aaacaccagt tccccgtcac cgaagccgac cttcgtctcg
     1561 ataacggccg gatccaaacg aaatattatc cgcaccccct ggaacagatc caggtgggcg
     1621 cccgcctcac aagcaagggc ggttccctaa aggacctcga cctggccatc acccccgtct
     1681 ccttcctttt cgaaggccag ccctttgtcc tgaaggccga cctgcaaaat ttcaccgatc
     1741 tcaactactc ccttacctcg agcggtaccc tcgacctggg gcgtatccag aaattgtttg
     1801 cagtaaaaga ctataccatc accggcctgg tggacacccg gctctcactc cgcggccgcc
     1861 agagcgacgc cacctccgga cactacgacc ggctcttcaa tgaaggatcg ctaaaagtaa
     1921 aagacctgca ggtgcgctcc gaactctttc ccctgccttt ttatatctat agcggggatt
     1981 ttcatttcca ccaggataaa atgtggttcg attcgtttaa cgcaagctat ggcaggagcc
     2041 ggtttacgct gaacggctgg ctctcggatg tcatcggcta tatgtccaat aagggacaac
     2101 cgctgaacgg aaatttcact ctcaacagcg acctcctgtt cgttgacgag ttcatggcat
     2161 tcgcggacag gacacccacc gctaccacct ccgccaccgg tacttccgcc accagcacca
     2221 ccagcacagg caccgccccg gccacctccg gcgtggtcat ggtcccggcc gatctttcgg
     2281 taggctttca tgccgatata aaaaggatcc agtacaatgg cctgaatatc gatagcttca
     2341 aaggcggcgt ccatctcgac agcggcgccc tccacctcga taccacgacc tttaccctcg
     2401 ccggcgcacc cgtacagatg aacgcttcct acacaagccc cgacccccac cgggccttct
     2461 tcgactacca cctcatcgca aaagacttcg acgtccagcg cgcctaccgc gaagtaaaac
     2521 tgtttcacga cctggccagc tccgccgcaa aggcacaggg catcatctcg ctcgactaca
     2581 aattgggcgg ccggctcgac gacaacatgc gccccgtata cccctcctta aaaggcggcg
     2641 gcacactgtc catcaggaaa gtaaaagtaa aagggctaaa actctttggg gaggtcagca
     2701 aggagaccaa caaggatgtc aacgatcccg atctctcgaa ggtggatata aagtcgacga
     2761 tcaataataa cctcatcacc atcgaacgca cccggatgaa ggtctccgtc ttcaagctgc
     2821 ggatagaagg ccaggccagc ttcgacggac gcctgaacct gcactgtcgc gtaggcctgc
     2881 cgcccttcgg tatcatcggt atccccgtat ccgtcaccgg tacccaggac aacccaaagg
     2941 tcaaggcccg ccgtgagacc gacaaggacg aactcgaagg aacggaagac accgctcagt
     3001 aataacgacc aatggccgga tcatccacga tgatcttctt caacgccgca gcatcgatgg
     3061 gcccatcctt tgcatagatg acctttcctc ccggctcgat caccatcgta tacggcaacg
     3121 ctccctgcca cttcggatcg atagcctcga tcagcttata cttgtcatcc acattgaaga
     3181 tatagttggt ggcggaggcc tgtacccctt tcaggaactg cagggccttg tcccgcttgg
     3241 caggttcgtc ggcactgatg ctgataaact cgaaatcccg gcggcgatac atccggttga
     3301 tgtccacgaa gtcggagaat tccgcgacac agggcccgca ccacgtggcc cagacatcga
     3361 tcacccggag cttctctgtc ttgttccgga tcagctcacg gatgccatcg gcgctgatcg
     3421 tatccagctt taccggttcc tttgcccatt ccgccttagc cttttcgatc cagtcgcttt
     3481 tctccgccca cttgatcgag cagccaaaga ccttcgtcgt ggtgaccgga acctccttat
     3541 tgtttaacag ggcttcgatg gcattgcggg tatccagcgt atgcggggtc tttgttggct
     3601 tctccacatc gtcgacccgg ccgctatacc gcagttttct gtccttgtcg aagacgaata
     3661 catgcggggt ggcgatcggc ccatatttaa gggaaacggc ctccgtctgc ccgtcataga
     3721 gataggggaa gttgaacttc ttctccgagg cccggcgctt catctcttca aagctgtccc
     3781 ccatatccgt atatcccagc tcatcgagcc ggatggactg cggatcattg ggcatgatcg
     3841 cgacaaccgc aacccccctg tccctataat cctttgtaag ccggatcaac cggtcctcat
     3901 acgcctgggc cgtagggcaa tggttgcagg tgaacaccac caccagcacc cgggcattcg
     3961 caaaagagga aagtgcatag gtcttcccat cgacaccggg aagcgagaaa tcgggggccg
     4021 cagcgccgat ggcaagggtg gggtggtcgc cggcgggttg cccgggactg tgatcggccg
     4081 gccggcaaaa ggcggcaaac aaaaaaagac aggcgaagaa gatggcaggc ttcatatggc
     4141 caatttttac taaaaggtac aactttaatc gacctgtcga cttgccagga cgtcatgatg
     4201 tagtaatgat gtagtgaaaa atacagcgga aatcctaact ttagcggcgc aacgaattct
     4261 gtcatataaa aaaaccgatc atgcacacga gaagatcctt cctcaagacc tccgctctct
     4321 attctgccgg catcctggcg gcgccggccc tcccctcctt cctggccgcg gccggcccgg
     4381 cgatcgggct gcaactctat acggttcgcg atgccatggc cgccgatccc gccgggacac
     4441 tcgccaaagt ggcgcagatc gggtacaact ccctggaagg agctacctat aataatggcg
     4501 tggagaaatt ctatgggatg gacccgaaga cctttgccgg cgaactaaag aacaacggcc
     4561 tggtcatgcg cagttgccac taccggctcg gagaagatcc ccagggcgca accctgacca
     4621 acggcgttta caacggcacc atcctgcacg actgggacaa ggccgtcgag gacgcccata
     4681 ccctcgggct ccaatacatg gtctgtgcct ggctctctcc aaaagaacgc ctcggcctcg
     4741 atcactataa aaaaatggcg aacgacttta acatcgccgg cgagaaatgc agcaaggccg
     4801 gtatccagtt ctgctatcat aaccatgact tcgaattcga cgcccagaac gggaaatatc
     4861 catatgatgt attgctcgaa agcaccgacc ccaaatcggt aaagatggag atggacctct
     4921 actgggtcac caaggccggt caggacccgg tcgccctctt taacgcccac cccggccgct
     4981 tccctctctg gcatctgaaa gacatggata gcacacccca gcatgccttc accgaagtcg
     5041 ggaatggaac catcgacttc aaaaagatct tcgccagcaa atccacgtcg gggatgaaat
     5101 atttcttcgt cgaacaggat cggtgccccg gttcgccctt cgacagcatt acaaagagca
     5161 ttacctatat caaacagaat ttggtgtaat actgtccttg ccccgggcat gcagctccat
     5221 aagaaaatcg aataaattcg tctcggtgga gatcttcagc tttttgcgca ggcgatagcg
     5281 gctgatctcc acccctctta ccgagatatt ctccaattgc gcgatctcct tgctgctcag
     5341 gttcatccgg agataggcgc aaagtttcag ctcatgtgcg ctaagcgccg ggtacgccgt
     5401 cttcaggatc cgcaggaagt cactgtgaac cttgtcgaag tggatggcaa actgctccca
     5461 gtccttgtcc atcctgttct cctccccaag gatccgcaac atcttcttga actcgtccgc
     5521 cgctaccccc tccccgttgc cccccttctt catccgcaaa agctcttccc ggatattgcc
     5581 cagcagctcc cctttctgta cgaggtgcat cgccgccgaa gccagttcgg tgttcttatg
     5641 ctctatctcc gcctccagct tctcattctt caacttgacg atctcttttt ccgacttctc
     5701 catctccagc tgatgcaggt attgcaaccg cgcctgttct tcctcgtgtt tcttctgctg
     5761 ccgccggaag atccgccgct gccagaaata caaaagacaa agaagcaccg ccagcaacag
     5821 cacataaagg ccataggccc agccggtctg gtaccagggc ggtaatatcg taaaggtata
     5881 ggtgctgatc gcggattcat tgcgcaggtt gctcttcgac ctcacctgga aggtgtagac
     5941 acccgccgga agattggtat attccttctc cgtcttcttc gaccattccg accagtcccg
     6001 gtcaaaaccc ttcaggcaat aactatattg gatactgctt tgcgccgcat aaagggtgct
     6061 ggtgtattcg aaatgcagcg aattccattt gttggagatg ctgtagatag cggaagatgg
     6121 ctgttccagg ctgtcatcca cctctccgta atatccgcca tacaggagac tgtccgtttt
     6181 gccaaaggcg cggacgctcc tgatccgcac ctggatagga tagtggttct tcttatactc
     6241 ctcgtagttg atatgataga accccttctc cgcgcctaca aagacattgt atttattgta
     6301 aggatcgata tgctcataat ccgcaacgag tttcccattt agctcgggga agaagatgat
     6361 ctccggctgc gaaccggaaa agtcgatgac ccccaggctg ttgtcctcta tgaaccagat
     6421 attgcccgcc ccatcctcct ttagcgcccg gatattcctt tccccgaaaa atccct
//
LOCUS       DATGFG010000808         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_74354, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000808 DATGFG010000000
VERSION     DATGFG010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74354"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VKQ52_15215"
     CDS             complement(<1..1207)
                     /locus_tag="VKQ52_15215"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLZ88603.1"
                     /translation="MMQRILFCLLSVLAVGVGYAQMPPYGGKLKPEQANIDVRHYTID
                     LVLNIPYRSLAGYAIVQCNLKAPATGLLFDLMDSFRVSKVTVNGKPAEFTHEHHELHV
                     TGNWPAGAAVVRVDYAGRPMIAKRPPWDDGFTFTHDSTGHPWVAVTAEGTGGKLYFPA
                     KDHPSDEPDEGVDMTIAVQKGLVVAGPGLLVGVRTSGGLSHYHWKTNYPINNYSIVFN
                     AGDYTVVNRTYTTIEGRTVPMQFYVLKEHAGSAPHLLEMMDHAAHIKEKYFGEYPWAK
                     EKIGLVETPHLGMEHQTMIAYGNKFHYTIVGGQDFDWLMNHEFGHEWWGNKVTAGDWA
                     DYWIHEGIGTFGDVLYTREMEGEAAYERYFQQVAPRIRNDKPIVLGKDIEEEEAYHPD
                     IYPKGAFFMH"
     gene            complement(1228..2403)
                     /gene="uxuA"
                     /locus_tag="VKQ52_15220"
     CDS             complement(1228..2403)
                     /gene="uxuA"
                     /locus_tag="VKQ52_15220"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HLZ88604.1"
                     /translation="MKRPFLMEETMRWYGPNDPVSLWDIRQAGCTGVVTALHHVPVGE
                     VWSVEEIKKRKAEVEGAGMRWTVIESLPVHEDIKKQTGNYKQYIANYQESLRNVAKCG
                     LEVVTYNFMPILDWMRTDIAYEMPDRSLALRFEKAAFIAFDLFLLKRPGAEKDYSAAE
                     IAAAEKRLSTMTAAEKETLYRNTLLGLPGSEERFTEEQILAALKTYAGIDAVKLKQHL
                     FYFLQQVVPVAEAGGLKMAIHPDDPPYPILGLPRIVSTERDARELLDAAPSPANGLCF
                     CTGSYGVRADNDLPGMVRRLGDHIHFIHLRSTKRDAGGNFFEADHLGGDVDMVAVIRE
                     LLAVQEKRSISLPMRPDHGHQMLDDLKKKTYPGYSAIGRLRGLAELRGVQWGLAYGG"
BASE COUNT          533 a          836 c          622 g          560 t
ORIGIN      
        1 tatgcataaa aaacgcgccc ttgggataga tatcgggatg ataggcctcc tcctcttcga
       61 tatccttgcc gagcacgatg ggtttgtcgt tccggatgcg cggcgccacc tgctggaaat
      121 agcgctcata cgccgcctcc ccttccatct cccgggtata caggacatcc ccgaaagtgc
      181 cgatgccttc atggatccaa tagtcggccc aatcacccgc ggtaacttta ttaccccacc
      241 actcgtgacc aaactcatgg ttcatcagcc agtcgaaatc ctgcccgccg acgatcgtat
      301 agtggaactt attcccatac gcgatcatcg tctggtgttc catcccgaga tgcggggtct
      361 cgacaagccc gatcttttcc tttgcccagg gatattcgcc aaaatatttc tccttgatat
      421 gggcggcatg atccatcatc tccagcagat gcggcgcact gcccgcatgt tccttcagta
      481 cgtagaactg catcgggacg gtgcgccctt caatcgtcgt ataggtccgg ttcacgacgg
      541 tataatcccc cgcgttgaac acgatgctat agttgttgat gggatagttc gtcttccaat
      601 ggtagtgact tagcccaccg gaagtcctga ccccgaccag cagcccgggt cccgcaacga
      661 ccagcccctt ctgcacggcg atggtcatat ccaccccctc atcgggctcg tcggaaggat
      721 ggtccttggc cgggaaataa agctttcccc ctgttccctc ggccgtaacg gcgacccacg
      781 gatgccccgt cgaatcatgg gtgaaagtaa acccgtcatc ccagggcggc cgcttggcga
      841 tcatcgggcg accggcatag tccacccgga cgacggcagc ccccgccggc cagttaccgg
      901 taacatggag ttcgtgatgc tcatgcgtga attcggccgg cttcccattc acggtcacct
      961 tcgacacccg gaacgagtcc atcaggtcga acagcagccc ggtcgcgggc gccttcaggt
     1021 tacattgaac gatcgcatac cccgcaaggc tcctgtacgg aatattcagc acaagatcga
     1081 tggtataatg ccggacatcg atattcgcct gctcgggctt gagctttccg ccatagggcg
     1141 gcatctgggc atagcccacg ccaacagcca gcacggacag aagacaaaaa aggattcgtt
     1201 gcatcatggt atgtcgattt aaccacccta ccccccatac gcaaggcccc attgcacgcc
     1261 ccgcagctcc gccagcccgc gaagccggcc gatggcgcta taccccgggt aggtcttctt
     1321 tttcagatcg tccagcatct ggtgcccatg atcgggacgc atcggcaggg aaatgctcct
     1381 cttctcctgg acggcaagca attcgcggat caccgccacc atatcgacat ccccgccaag
     1441 atgatcggct tcgaagaaat tccccccggc atcgcgtttg gtgctccgca gatggataaa
     1501 atgaatatga tcccccaggc gccggaccat ccccggcaga tcattgtcag cacggacacc
     1561 atagctgccc gtacaaaaac aaaggccgtt ggcaggcgaa ggggcagcgt ccagcagttc
     1621 acgggcatcc cgctccgtac tcacgatacg gggtaatcct aatataggat agggggggtc
     1681 gtccggatgg atcgccatct tcagcccccc ggcctcggcc accgggacca cctgttgtaa
     1741 aaaatagaac aaatgctgct tcaatttcac cgcatcgata cccgcatagg tcttcaacgc
     1801 cgcaaggatc tgttcctcgg tgaaccgctc ttcactgccc ggcaatccca gcagggtatt
     1861 gcgataaagc gtttccttct cggccgccgt catcgtgctc agacgtttct cggccgcggc
     1921 gatctcggcc gcgctatagt ccttctctgc gcccggtctc ttcaacagga aaagatcgaa
     1981 ggcgataaaa gcagcttttt caaaccgtaa cgccaggctc cggtcgggca tctcataggc
     2041 gatatccgtc cgcatccagt cgaggatcgg catgaagtta tacgtcacga cttccagccc
     2101 gcatttggcc acattgcgca ggctctcctg gtagttggcg atatattgtt tatagttgcc
     2161 ggtctgtttc ttgatgtctt catgcaccgg caggctctcg atcaccgtcc agcgcattcc
     2221 ggcgccctct acctcggcct ttctcttctt tatctcttcc acggaccaca cctccccgac
     2281 gggtacatga tgaagggccg tgaccacacc cgtacatccc gcctgacgaa tatcccatag
     2341 acttaccgga tcgttcggac cataccatcg catggtctct tccattaaaa agggtctttt
     2401 catatatacg cttcttcgat ttaacagact ccctacattc cacaggctgc caccgcccgg
     2461 cgccccggtc atttcgctat aacctcgatc tccgtgctca cccccttcgc cgctccaagc
     2521 gcctcactcc tcgcagtcgg caggcttccg c
//
LOCUS       DATGFG010000809         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1528338, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000809 DATGFG010000000
VERSION     DATGFG010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528338"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..632)
                     /locus_tag="VKQ52_15225"
     CDS             complement(87..632)
                     /locus_tag="VKQ52_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88605.1"
                     /translation="MRIALLCICLVSLAPGCRPQQRQEREALPWVVLEDIPRDSLGRV
                     TAAPRWQPPIWTNRATADTFVVFGAGDSVLMISNDGFPARMFVPTDVGARHWKPRPSI
                     GRSLRLMGTYERRDLRGIFQCFYITPDRHNLLQVWYTNPGNVMDALLFSKDSSNILEQ
                     EKLVIDPRADTSEAEAAVPVR"
     gene            complement(636..1595)
                     /locus_tag="VKQ52_15230"
     CDS             complement(636..1595)
                     /locus_tag="VKQ52_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88606.1"
                     /translation="MQKKHLSCCSLLALTALLLCNACSKSNRMANPSRASRSMNLVTP
                     TRRFIPLTKDAFHLVQQAQRQGIGATAIERLLDSLAAVIPARRTVNSITTLDDPPGSD
                     VPSTDELDGAAQDFGEAASFNDYETQQLVDQWAGDLPEDWMDLYNATNPSFSSVFNMQ
                     LTFPKRGNTVQVTVPYTFTAVLGAGAMYQLTGIGPSTFPPQLLPVGAGWGDVTQIDSY
                     LNGLTNNMYVHADAQEKRTEIVSTGGTIEISANANAGIYQVGAKIGTNFTVQKAFNIY
                     NQYTMDASLNAYIGVSFNVPYGAPSCQVWGTVTCVDTGILLNN"
     gene            complement(1606..4125)
                     /locus_tag="VKQ52_15235"
     CDS             complement(1606..4125)
                     /locus_tag="VKQ52_15235"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HLZ88607.1"
                     /translation="MYLRFAKAGAALTALIASFFLTLPTTTLAQNIDTAAIKALAAGK
                     KDTAKSKLKPYKDVIPATAKTSRGLFTIHKVDDRWLAEIPDSLFGRDMLVVTRIVKAP
                     TGYYSLAKVFGALSYAGDAINNNVIRLERLTDDKVFLRSISYLERSEDSSANGLYQNV
                     QDNNAQPIDAAFPVKAINEPGRSVVIDLTDFINSENDVLYFNGQLKPLLGLTALAADR
                     SFINTIKAFPTNIEIKTVRTYNDAPGKGAHSYELNSSIVLLPSVPMRSRPADVRVGLF
                     YDSYIDYDADPQSVRPGNHIWRWRLEPRPEDRERYLHGELVEPAKPIVIYIDPETPRK
                     WVPYLIQGINDWQAAFEQAGFKNAIVGKEAPVGDSTWSMEDARHSVLLYHASPVENAN
                     GPSIRDPRTGEILETHINWFHNVMQLLYDWYFIQAAAVDPRARHAHFDDTLMGNLVRF
                     VSSHEVGHTLGMQHNFGASSTVPVDSLRSKAWVDAHGHTPSIMDYARFNYVAQPEDGI
                     DESGLFPRIGDYDKWAIRWGYRWLPPSISATTEKDTLNAWIVRQLAGGPQYFYGAQTN
                     PFNPMGSPSNPDPRDQSEDLGDDAMKAGDYGIRNLKRIEPHLMEWTMDGHDDYGKLEH
                     MYQSLVGQYGRYMGHVLKNIGGIMITPKSPGQQGPVITYPSKEKQQQAMAFLQTQLFT
                     TPTWLIDEKLYSLGAGDFDAVARVQFGVINSLFDASRLVQLTQQEGALGSKGYTVAGM
                     LHDLQNGLFSELSAGKPIDAHRRRLQKYYVNKLVETLGADTAVRREIQSTSDVFPSLK
                     AAARELAESIKQATLRYPAGAMHEHLTDLYDRLEWTLKPRN"
     gene            complement(4144..>4410)
                     /locus_tag="VKQ52_15240"
     CDS             complement(4144..>4410)
                     /locus_tag="VKQ52_15240"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88608.1"
                     /translation="VRTRAGLAATTASTQPDLLAAILRERRVEFFTEGHRFYDLRRTG
                     NLDALMTVLAPLKGGAWASFKAWWPIPVGDIQNDIHLTQTPGFQ"
BASE COUNT          839 a         1195 c         1397 g          979 t
ORIGIN      
        1 atatcaatcg ttttttggag ttcttttaaa aaaacctccg cctggtgggc ctggttaaag
       61 cctaggcgga ggtccacatc gggatgttat cggaccggga ccgcagcttc cgcctctgac
      121 gtgtcggccc tggggtcgat gaccagtttc tcctgttcca gtatgttcga cgagtctttg
      181 gagaacagga gtgcgtccat gacattaccg gggttcgtat accatacctg gagcaagttg
      241 tgcctgtccg gggtgatata aaagcactgg aaaatgcccc tgaggtcgcg gcgctcatag
      301 gtacccatca gccgcaggga tcgcccgatg gacggccggg gcttccagtg ccgggcgcct
      361 acgtcggtgg gtacgaacat tctcgccggg aatccgtcgt tggaaatcat caggacgcta
      421 tcgccggcgc cgaaaacgac aaaggtatcc gccgtcgcgc ggttggtcca gatcggcggt
      481 tgccagcggg gtgcggcggt gacccgcccc aggctgtccc tgggtatgtc ttcaaggaca
      541 acccagggca gcgcttcccg ctcctgccgc tgctgcggcc ggcaaccggg ggccagtgaa
      601 accaaacaaa tacaaagaag ggcgatgcgc atgtcttagt tgtttagaag gatcccggtg
      661 tctacacatg tcacggtgcc ccacacctga caggacgggg ccccataggg aacgttgaag
      721 gatacgccga tataagcgtt cagggaagca tccatggtgt actggttgta gatattgaac
      781 gccttttgaa cggtgaagtt ggttccgatc ttggcgccta cctggtagat accggcgttg
      841 gcgttcgcgc tgatctcgat ggtgccaccg gtggagacga tctccgtccg cttctcctgc
      901 gcatccgcgt gcacatacat gttgttggtg agaccgttca ggtagctatc gatctgcgtc
      961 acgtcgcccc atcccgctcc cacgggtaac aattgcgggg gaaaagtcga gggcccgatg
     1021 cctgtcaact ggtacatggc cccggccccc agcacggccg tgaaagtata agggacggtc
     1081 acctggaccg tgttgccccg tttgggaaag gtaagctgca tgttgaatac cgacgaaaac
     1141 gaaggattgg ttgcattgta taaatccatc cagtcctcgg gcaagtcgcc agcccattgg
     1201 tccaccagtt gttgggtttc gtaatcgttg aaggaagcgg cttcgccgaa gtcctgggcc
     1261 gcgccgtcga gttcgtcggt ggaaggcacg tccgaaccag gaggatcatc cagggtcgtg
     1321 atggaattca ccgtgcgccg ggccgggatg acggcggcga gagaatcgag cagccgctcg
     1381 atggcggtcg cgccgatgcc ctgtcgctgg gcttgttgca ccaggtgaaa ggcatctttg
     1441 gtcagcggta tgaaccgacg tgtgggtgta accaggttca tggaccgcga tgcgcgggac
     1501 ggatttgcca tacgattgga tttcgaacag gcgttgcaca gcagcagggc ggtgagcgcc
     1561 agcagagaac agcaggaaag gtgctttttt tgcatatttg gaggtttaat ttcggggttt
     1621 aagggtccat tccagtctgt cgtacagatc cgtcaggtgt tcgtgcatcg ccccggcggg
     1681 atagcgcagg gtcgcctgtt tgatcgattc cgccagttcc cgggcagcgg ctttcaggct
     1741 gggaaacaca tcgctggtgg attggatctc ccgtcttacc gcggtgtcgg cgccaagcgt
     1801 ttcaaccagc ttgttgacat aatatttctg gaggcggcgc cgatgcgcat cgatgggttt
     1861 gcccgcagac agttcggaaa agagcccgtt ttgcagatcg tgcaacatcc cggctaccgt
     1921 atagcctttc gagcccaggg ctccctcctg ctgtgtcaac tgcacgagcc gggaggcgtc
     1981 gaaaagactg ttgatgacac cgaactggac tctcgccact gcatcgaaat cccccgcacc
     2041 gaggctgtac agcttttcgt cgatgagcca ggtgggggtg gtgaacaact gcgtttggag
     2101 gaaggccatc gcctgttgct gcttctcctt gctgggatag gtgatgaccg gtccctgctg
     2161 gccgggcgat ttgggggtga tcattatgcc gccgatattc ttaaggacat ggcccatata
     2221 gcggccatac tgcccgacca gcgactgata catatgctcc agcttcccgt aatcgtcatg
     2281 gccgtccatg gtccattcca tcaaatgggg ttcgatcctt ttcaggttgc ggataccgta
     2341 atcaccggcc ttcatggcat cgtcgccgag gtcttcgctc tggtcgcggg gatcagggtt
     2401 actgggactc cccatgggat taaagggatt cgtttgggcg ccatagaaat actgtgggcc
     2461 gccggccagt tgccggacga tccaggcatt cagcgtgtcc ttctccgtgg tcgcgctgat
     2521 ggaaggcggc agccagcgat agccccagcg gatcgcccat ttgtcatagt cgccgatgcg
     2581 cgggaagagc ccgctctcgt cgatgccatc ctccggttgg gccacataat tgaaccgggc
     2641 ataatccatg atggaaggcg tatggccatg ggcatccacc caggccttgc tgcgaaggct
     2701 atccaccggc acggtggagg aggcgccgaa attgtgctgc atgcccagcg tatgacccac
     2761 ctcgtgagag gatacgaagc ggacgaggtt ccccatcagg gtgtcgtcga agtgggcatg
     2821 gcgggccctt gggtcgacgg cggccgcctg gatgaaatac cagtcgtaga gcagctgcat
     2881 cacgttgtgg aaccagttga tatgcgtctc gaggatctcc ccggtgcggg gatcgcggat
     2941 gctggggccg ttggcattct ccacgggcga ggcgtgatag agtaataccg aatgccgggc
     3001 atcttccatc gaccaggtgg aatcgcccac cggcgcttcc ttaccgacga tcgcgttctt
     3061 aaatcccgcc tgttcgaagg cggcctgcca gtcattgatc ccctggatca ggtagggcac
     3121 ccatttcctg ggcgtctccg ggtcgatata gatgacgatg ggcttcgcgg gttcgaccag
     3181 ttcgccatga agatagcgct cgcggtcttc cggcctgggt tccagccgcc agcgccagat
     3241 atggttgccc gggcgcacac tctgggggtc tgcgtcatag tcgatatagc tgtcgtaaaa
     3301 gagcccgacg cgcacatcgg ccggccggct ccgcatcggt accgacggca ggaggacgat
     3361 gctgctgttg agctcatagc tgtgggcgcc tttccccgga gcgtcgttgt aggtgcgaac
     3421 ggtcttgatc tcgatgttcg tgggaaaggc tttgatggta ttgataaagg accggtcggc
     3481 cgccagcgcc gtcaggccca gcaggggctt cagctgacca ttaaagtaga gcacgtcgtt
     3541 ctccgaattg atgaaatccg tgagatcgat gaccaccgac ctgccgggtt cgttgatcgc
     3601 cttaacgggg aatgccgcat ctatcggttg ggcattatta tcctgtacat tctgatacaa
     3661 accgttggcc gagctgtcct cgctcctttc gagatagctg atgcttctga ggaagacctt
     3721 atcgtccgtg agccgctcca gccggatcac gttgttgttg atagcgtccc cggcatagct
     3781 caaggctccg aagacctttg ccagactgta ataaccggtt ggcgccttga cgatgcgggt
     3841 gacgacgagc atatcacggc cgaacaggga atcggggatc tccgccagcc atcggtcgtc
     3901 cactttgtgg atagtgaaga gaccccggga tgttttggcc gtggcgggga tcacatcctt
     3961 atagggtttg agcttcgatt tggcggtgtc cttcttgccg gcggcaagtg ccttgatggc
     4021 cgcggtgtcg atgttctggg ccagggtcgt tgtcggcagt gtaaggaaaa aggaggctat
     4081 gagggccgtg agtgcggccc ctgcttttgc aaaacgcagg tacatgttaa tggatttttt
     4141 tgtttattgg aaacccgggg tttgagttaa atggatgtcg ttttgaatgt cgccgacggg
     4201 tatcggccac caggccttga aactggccca ggcgccgcct ttcaggggag ccagcaccgt
     4261 catgagggca tccaggttac ccgtgcggcg gaggtcgtag aagcggtggc cttccgtgaa
     4321 gaattcgacg cggcgttcgc gcaggatggc ggccagcaga tcgggctggg tggaggccgt
     4381 ggtggcggcg aggccggctc ttgtccttac
//
LOCUS       DATGFG010000810         9225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1262682, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000810 DATGFG010000000
VERSION     DATGFG010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262682"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VKQ52_15245"
     CDS             complement(<1..651)
                     /locus_tag="VKQ52_15245"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HLZ88609.1"
                     /translation="MKTITGWAALTALACSFATIVRAQISTPPKWEVGLGAGAFIYQG
                     DLAPSFAGSYKLIDPGVSLSLSRLINRHLALRTGVTVAGLRGDDRKYDDEAWRQARAL
                     RFKTSVFEVAESVVWDVFGNNDDRTRTRFSPYVFAGVGYSFLRVRRDASRFDSSFFGP
                     HPGIINGLKADLATSPPRGVVVVPVGAGVRYTVTPAWSINLEANYRFSETDYLDGFS"
     gene            complement(717..1475)
                     /locus_tag="VKQ52_15250"
     CDS             complement(717..1475)
                     /locus_tag="VKQ52_15250"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HLZ88610.1"
                     /translation="MNRITGQVAVTALACFFATIVQGQISALPKWEVGLGAGPFLYQG
                     DLAPSALGSYKTLRPGISIFGSRLLNRFLALRTSLTFTGLSGDDLKYGNVAWREARGL
                     NFHTSVFEVSETVVWDLLGNNDNHYRTRFSPYVFGGIGYSFLRVRRDASRFNTSYFGA
                     ESTVTNGLTADLAHTMPRGIPMIPVGVGVRYSISPAWSISLESAYRFTFTDYLDGFSK
                     VGDPAKKDHYYGVTVGVIYTFLKSKELKCPRKVQ"
     gene            1789..2595
                     /locus_tag="VKQ52_15255"
     CDS             1789..2595
                     /locus_tag="VKQ52_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ88611.1"
                     /translation="MGITDRKLRQKEEVRASILDTAWEMVVTEGWQSFSIRKIADAIE
                     YSVPVIYSHFENKDAILLEFNRKGFQLLTDALITAKADQDNPAEQIRAMGRAYWHFAF
                     QNKEYYQLMFGLGIPTCETVSRIPAMGTFNEVITSTIIAMVPAGQKPAFNPFLKYQSF
                     WSMLHGLVSINMSAIGRTQKSGNEHDNLPLQVLEDVMCSFIRGIQAAEQSAATEHPAP
                     REHPTTTEHPAPREHPPTAEHPAPREHPTTTEHPASRTTPEHPNENPISG"
     gene            2707..3336
                     /locus_tag="VKQ52_15260"
     CDS             2707..3336
                     /locus_tag="VKQ52_15260"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLZ88612.1"
                     /translation="MHLLEALKWRYATKRMTGEKLPQAKVDVILEAVRLAPTSNGIQP
                     FTILHITDKQLLEKIRPIANNQPQITEASHLLVFAAWTDVTSEHIDAVYDRIASEREL
                     APGAQNDYVDKLKTRFAGYTPEFRAQWAARQTYIALGIAITAAALEKVDASPMEGFRN
                     ADLDQLLGLPQKGLRSCALMALGYRDLANDKMVQLKKVRRPKEELVIEL"
     gene            3413..3985
                     /locus_tag="VKQ52_15265"
     CDS             3413..3985
                     /locus_tag="VKQ52_15265"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ88613.1"
                     /translation="MKKIILSTAALFLLAGSTFAQEWNMDKAHSQINFGITHMGINEV
                     DGSFGSVTAKFTATKDDFSDAVIELTADINSLNTGNEQRNNHLKSPDFFNAAQFGTLT
                     FKSTSFKKVSDKAYELTGDLTLHGVTKPVTLTATFNGTTTNPMNKKTVAGFRVTGTIK
                     RNDFGIGAGFPTAMLSDEVTLVANTEFVKG"
     gene            4041..4574
                     /locus_tag="VKQ52_15270"
     CDS             4041..4574
                     /locus_tag="VKQ52_15270"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ88614.1"
                     /translation="MNKYIAPLTALLLLAGSAFAFIAAQSWQIADSYSIAFSSDDAGG
                     VFKGFKGAIAFDEQNPAASRFDVTIDAATINTGNGLQNKHAKSDEWFDVAKYPQIHFQ
                     SKNITKAANGYQVTGDLTLHGVTKTITFPFTFKKTGAGGLFVASFTVNRNDYKLGKPG
                     KDVGEDIKLDISVPVTK"
     gene            4598..4987
                     /locus_tag="VKQ52_15275"
     CDS             4598..4987
                     /locus_tag="VKQ52_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88615.1"
                     /translation="MFKKALLLGIVSGLLAGIAGVIYARIYYSANEADFSKVVSTVHI
                     VAASLFGGVLAAIGYTVLDKLLKANGEIVFNFIFTILSFASLLLPIGHRFSPPIDTPE
                     LFPGMVIPMHFFPALGWYTLKPLFIKK"
     gene            5228..5608
                     /locus_tag="VKQ52_15280"
     CDS             5228..5608
                     /locus_tag="VKQ52_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ88616.1"
                     /translation="MNKRVLILDDDLDILQICTIVLRKKGFDVQTLNNSNQVVNQVKA
                     WHPDVILMDNWIPGPGGIEATRLLKLAPETQDIPVIFFSANSNVTQLAHEAHADYFLQ
                     KPFDITELEAIVQMALTPPEAARP"
     gene            5745..6977
                     /locus_tag="VKQ52_15285"
     CDS             5745..6977
                     /locus_tag="VKQ52_15285"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HLZ88617.1"
                     /translation="MSTSSAPLPLTATAAARPARTRRVESIDLLRGLVMIIMALDHAR
                     DYFNNSAYLYDPTDLSQASPALFLTRWITHFCAPIFMLLAGIAAWLYGNKNGRKAVSF
                     FLLTRGAWLIFVELFIVTVGWTFNIHFHFFILQVIWAFGVAMVLLSALVHLSRPVLLT
                     VALLLICGHDLLDGIHATGNSLSAVGWSFLHQFQFFSYEHFSFVIGYPILPWLGIISL
                     GYCLGQLYAPEVDPARRKKILRALGLGAIAGFILIRAINVYGDPNPWSTQHNTLYTVL
                     SFINVSKYPPSLLYILMTLGPGLLFLSVAERPLNKITAPIAVFGRVPFFYYILHIYLF
                     HVIAVLAAMAQGHPASQMTNLTNWVTNNPQLKGYGFDLWGVYLAWIVVVLALYPLCKR
                     FDKYKRSHQAQKKWLSYL"
     gene            complement(7088..7654)
                     /locus_tag="VKQ52_15290"
     CDS             complement(7088..7654)
                     /locus_tag="VKQ52_15290"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HLZ88618.1"
                     /translation="MSQDKLLIIGGSAGSLQVILPLLAALGKDYPMPVLVVLHRNGGF
                     ESSLEELLSSRTHQVIKEVEEKELLRPGIVYICPADYHVLLEQDRSFSLDYSERVNFS
                     RPSIDVTFRSAADVYGEGVIALLLSGGNADGAEGMEYVKSRGGFTIVQDPRTAEVPYM
                     PQQALRRVVVDVVAATEELPGIVRALGR"
     gene            complement(7641..8471)
                     /locus_tag="VKQ52_15295"
     CDS             complement(7641..8471)
                     /locus_tag="VKQ52_15295"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HLZ88619.1"
                     /translation="MEGYQLQNEELQLLLNDIYTRYGYDFTEYSKASLQRRVQRLFAL
                     DKFPSFAEFRYRLQHDSQYFRRFVEEITVNVTEMLRDPSFYRTLRMTILPVLATYPFI
                     RIWHAGCSTGEEVYSMAILLKEEGLLHKSLLYGTDINPLVIEKARKGIFPVSQMQKYS
                     ENYIQSGGLRDFSSYYTANYHLAKFDSSLTGKMIFSTHNLVSDFSFNAFQLILCRNVL
                     IYFERELQTKVFGLFDYSLENFGYLALGSKESLRFSPMADRYRQVGREKIWRKVHEPG
                     "
     gene            complement(8475..>9225)
                     /locus_tag="VKQ52_15300"
     CDS             complement(8475..>9225)
                     /locus_tag="VKQ52_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ88620.1"
                     /translation="FLGTHNLHMEIAPTIGQSADILQKEGVHGVILSREAGKEEEDTL
                     EMVRKNAAFENIPIIVFTGINISKAEEARLRQYADSIVVKTAHSYQRVLDEVSLFLHV
                     VSEGKEAGANGNGAGRLGVLDQVLRDKSVLVADDDVRNIFSLTKALEQHKMKVVTAMD
                     GKEALGVAEGGGVDIVLMDMMMPEMDGFEAITRIRRNPRLRRLPIIAVTAKAMAGDRE
                     RCIQAGASDYISKPVDIDQLLSLLRIWLYEK"
BASE COUNT         2289 a         3187 c         1982 g         1767 t
ORIGIN      
        1 gctgaaccca tccaggtagt ccgtctcgct aaaccggtaa ttggcctcga gattgatcga
       61 ccaggccggc gttaccgtat atcgcacccc ggcgcccacc ggcaccacca ccacaccccg
      121 cggcgggctc gtcgccagat cggccttcaa tccattgatg atacccggat gcggtccgaa
      181 aaaactggaa tcgaaccggc tcgcatcccg ccgtacccga agaaaagaat atcccacgcc
      241 cgcaaataca taaggcgaga accgcgttcg ggtacgatcg tcgttgttgc cgaagacatc
      301 ccacacgaca ctctccgcaa cttcgaatac cgaggttttg aagcgaagcg cccgcgcctg
      361 ccgccaggct tcgtcatcat acttccggtc atccccacgg agccccgcca ccgtcacacc
      421 ggtccgcaaa gccagatgac gattgatcaa ccggctcagg gaaagcgata ctccgggatc
      481 gatcagttta tatgaaccgg caaaggaagg cgccagatcg ccctggtaga tgaacgcacc
      541 cgcgccaagt ccgacttccc atttgggtgg ggtagatatc tgtgcacgaa cgatggtagc
      601 gaaagaacaa gccaatgccg taagggcagc ccaaccggtg atcgttttca tgaatatgaa
      661 gatttatgta acgctatacc atcgggggcc atcaatacaa taacagggcc aatccgctat
      721 tgcaccttcc gcggacactt caactccttg ctcttcagga aggtatagat caccccaacc
      781 gtgaccccat aatagtgatc cttctttgcc ggatctccca ccttgctgaa cccgtccagg
      841 taatccgtga aggtgaaccg gtacgccgac tccaggctga tcgaccacgc agggctgata
      901 gaatagcgca ccccaacacc caccggaatc atcgggatac ccctgggcat agtatgggca
      961 agatcggccg tcaacccatt ggtgaccgta gactccgccc cgaaataact ggtgttgaac
     1021 cggctcgcat cccgccgcac ccggagaaaa gaatatccga tcccgccaaa tacatacggg
     1081 gagaaccggg tccggtaatg gttgtcatta ttgcccagca ggtcccagac caccgtctcc
     1141 gagacctcga aaaccgaagt gtggaaatta agcccccggg cctcccgcca ggccacattc
     1201 ccgtatttca ggtcatcacc gcttagcccg gtgaaggtca gactggtgcg gagcgccagg
     1261 aaacggttca acaaccggct gccaaaaatc gagatccccg gccgaagggt cttataggaa
     1321 cccagggccg aaggcgccag gtctccctgg taaagaaaag ggccggcgcc cagaccgacc
     1381 tcccacttgg gtaatgctga aatttgtccc tgtacgatgg tagcgaagaa gcaggccaat
     1441 gccgtgacgg caacctggcc ggtgatcctg ttcatgataa tagagtttag gtagtgctat
     1501 tccatcttct tcgccttgct gttcttcgct ttactaataa aagagcagga accgcgccgt
     1561 attgtaagca attgaacact ataacattac acatgcggcg aaaacccgga atccgccggt
     1621 gaaacacccc ggccccagcc cgcgccaagc cgctccaact cccaaccccc tcccgctgcc
     1681 ccatggtccc gcatcctcgc gccaaccctt ttccaaaaaa tatttggcgg aatgaaaaaa
     1741 gggtgtaatt ttgcctaacg ctgttacaaa aagtaactga ggtagaaaat gggtatcaca
     1801 gatcgtaaat taaggcagaa agaagaggtc cgggccagca tcctggacac ggcctgggaa
     1861 atggtcgtca ccgaagggtg gcaatccttc tccatccgca agatcgccga tgccatcgaa
     1921 tacagtgtcc cggtaatcta cagtcatttt gaaaataagg acgctatcct gttggaattc
     1981 aaccgaaaag gatttcagct gctgaccgac gccctcataa cggcaaaggc cgaccaggac
     2041 aaccccgccg aacagatcag ggccatgggc cgcgcctact ggcacttcgc cttccagaac
     2101 aaggagtatt atcaactcat gttcggcctg ggaatcccca cctgtgaaac agtaagccgg
     2161 atcccggcaa tgggcacctt caacgaggtc atcaccagca ccattatcgc catggtgccc
     2221 gccggccaaa aaccggcctt caaccccttc ctgaaatacc agtccttctg gtccatgctc
     2281 cacggcctcg tgtccatcaa catgagcgcc atcggcagaa cccaaaagag cggtaacgaa
     2341 cacgataacc tgcccctgca agtcctcgaa gatgtcatgt gcagtttcat ccggggcatc
     2401 caggccgccg aacaatccgc cgccactgaa cacccagccc ctcgggaaca cccgacaacc
     2461 actgaacacc cagctccgcg ggaacacccg ccaaccgcag aacacccagc cccccgggaa
     2521 cacccgacaa ccacggaaca cccggcctcc cggacaacac ccgaacaccc aaacgaaaac
     2581 ccaatatccg gctaaacccg cgcgcatttt tttttggcgc aaaacgataa cagtgttata
     2641 aataataatc ccggtaggcg caaccacgct tccggacaaa cgaaataaaa cctaacaaca
     2701 aactatatgc atctcctgga agcattgaaa tggcgctacg caacgaaacg gatgaccggg
     2761 gaaaaactcc cccaggcaaa ggtcgatgtc atcctcgaag ccgtacggct cgcgccaacc
     2821 tccaacggta tacaaccgtt cactatattg cacatcaccg ataaacaact gctggaaaaa
     2881 atacggccca tcgccaacaa tcaaccccag ataaccgaag catcccacct gctggtcttc
     2941 gcggcctgga cggacgtaac atccgaacac atcgacgccg tctacgaccg catcgcaagc
     3001 gaacgggaac tggcgccggg cgcacaaaat gactatgtag ataaactaaa gacccgcttc
     3061 gccggctata ccccggaatt ccgcgcacaa tgggcagccc gccagaccta tatcgccctc
     3121 gggatcgcca tcaccgccgc cgccctcgaa aaagtggacg cctcccccat ggaaggcttc
     3181 cgcaacgccg atctggacca gttgctcggc ctgccccaaa aaggcctccg cagctgcgcc
     3241 ctcatggccc tcggctaccg ggacctcgcc aatgataaaa tggtccaact gaaaaaagtc
     3301 cgccgcccca aagaagaatt agtaattgaa ttataaacta ttttcgtgtt gcaacattaa
     3361 acaacacgcg acgctaaaaa agccattctt aaaaacacac aaaaaccaaa aaatgaagaa
     3421 gatcattctc agcacagcag ctctctttct cctggccggc tccaccttcg cacaggaatg
     3481 gaacatggac aaagctcact cccagattaa cttcggcatc acccacatgg gcatcaacga
     3541 agtcgacgga agctttggct ccgtaacggc taaattcact gcgacaaagg acgatttctc
     3601 cgatgcagtc atcgaactca cggcagatat caactctctc aacaccggca acgaacagcg
     3661 gaacaatcac ctgaagagcc ccgacttctt caacgccgcc cagttcggta ccctcacttt
     3721 caaaagcacc agctttaaga aagtaagcga caaggcctac gagcttacag gcgacctgac
     3781 cctccatggc gtcacgaaac ccgtaaccct tacggctacc ttcaacggca cgaccaccaa
     3841 cccgatgaac aagaagaccg tcgcaggttt ccgggtcacc ggtaccatca aacgcaatga
     3901 cttcggcatc ggcgccggct tccccaccgc tatgcttagc gatgaagtta ccctggttgc
     3961 caacaccgaa ttcgtaaaag gctagtcttt ccccggtccc tgccgccaca aacggcaggg
     4021 actatctcaa aacaatcagt atgaacaagt atatagctcc cctcaccgcc ctcctgctcc
     4081 tggcaggtag tgccttcgcc ttcattgccg cacagagctg gcagatcgcc gatagctaca
     4141 gcatcgcctt tagcagcgac gatgccggcg gggtcttcaa ggggttcaag ggcgccatcg
     4201 ccttcgatga gcaaaacccg gccgcatccc ggttcgacgt gaccatcgac gccgcaacga
     4261 tcaacaccgg caatggacta cagaacaaac acgcaaagag cgacgaatgg ttcgatgttg
     4321 cgaaatatcc acagatccac ttccaatcca aaaacattac aaaggctgcc aacggctacc
     4381 aggtgaccgg cgatctgacc cttcacggcg tcacaaagac catcaccttc ccgttcacct
     4441 ttaaaaagac aggcgcaggt gggctcttcg tggcttcctt cactgttaac cgcaacgact
     4501 acaagcttgg caaacccggc aaagacgtag gcgaagacat caaactcgac atttccgtcc
     4561 ccgtcaccaa atagaaggca agactaaata gcaaaccatg tttaaaaaag cattactact
     4621 gggcatcgtc tcgggcctgc tcgccggcat cgccggggtc atctacgcca ggatctacta
     4681 ttcggcgaac gaagccgact tctcaaaggt cgtttccacc gtccatatcg tagcggctag
     4741 cctcttcggc ggcgtactgg cagccatcgg gtacacagtc ctggataagc tgctaaaggc
     4801 aaacggggag atcgtattca atttcatctt caccatcctt agcttcgcca gcctcctgct
     4861 gcccatcggc caccggttct cacccccgat cgacacgccg gagctcttcc cgggcatggt
     4921 catccccatg catttcttcc cggccctggg ctggtacaca ctcaaaccgc tattcataaa
     4981 gaaatagatc aaaagcaagg cattccataa gaaaaggcaa taaccaacca cgacccgggc
     5041 gcgcactccg caaccgggtc tcttttttcc cgcgaacccc gatccctgaa cccgctctcc
     5101 ccaatccccg aactcacgct ccctgatccc ggagcccggt ctttttatcc acctaaataa
     5161 ctgattttta tcccatcgcg tttgttggca caaaattttc gatagatatt tgcaataacg
     5221 tcaaccaatg aacaaacggg tattgatact ggacgacgac ctggatatcc ttcagatctg
     5281 caccattgtc cttcgcaaaa aaggcttcga tgtgcaaacc ctcaacaact ccaaccaggt
     5341 cgtcaaccag gtgaaagcct ggcatcccga cgtcatcctg atggacaact ggataccggg
     5401 ccccggcggc atcgaagcca cccgcctgtt gaaactggcc cccgaaaccc aggacatccc
     5461 cgtcattttc ttcagcgcca acagcaacgt cacccagttg gcacacgaag cccacgccga
     5521 ctacttcctc caaaaaccat tcgacatcac cgaactcgaa gccatcgtac aaatggccct
     5581 gacgccgccg gaggcggcgc ggccgtaagg ccagacattg gcgctgccgc cggaggccgt
     5641 acgccgtaag gctggactac cccctttatt tcccgcaggt agtccacatc cccttgcgca
     5701 ttggagcaaa cgactcatct ttgcggagct aaacacccca tgtcatgtca acatcctccg
     5761 cccccctccc cctcaccgcc acggctgccg cacgccctgc acgaacccgc cgtgtggaat
     5821 ccatcgacct ccttcgcggc ctcgtcatga tcatcatggc cctcgaccac gcccgcgact
     5881 acttcaacaa cagcgcctat ctgtatgatc caaccgacct gtcacaagct tccccggccc
     5941 tcttcctcac ccgctggatc acccactttt gcgctccgat cttcatgctc ctcgccggca
     6001 tcgccgcctg gctctacggc aacaagaacg gccgcaaagc cgtctccttc ttcctcctca
     6061 cccgcggcgc ctggctcatc ttcgtggaac tctttatcgt caccgtcggc tggaccttca
     6121 acatacactt ccatttcttt atcctgcagg tcatctgggc cttcggcgta gccatggtcc
     6181 tcctctccgc cctcgtccac ctaagccgcc ccgtcctgct aaccgtcgcc cttctcctca
     6241 tctgcggcca tgacctcctc gatggcatcc acgccaccgg caacagccta tccgccgtcg
     6301 gctggtcctt cctccaccag ttccagttct tctcctatga gcatttcagc ttcgtcatcg
     6361 gctaccccat cctgccctgg ctcggcatca tcagcctcgg ctactgcctg ggtcagctct
     6421 atgcaccgga agtcgacccc gcccgcagaa aaaagatcct ccgcgccctc ggcctcggcg
     6481 ccatcgctgg cttcatcctc atccgagcaa taaacgtcta cggtgacccc aacccctggt
     6541 ccacccaaca taacaccctc tataccgtcc tgtcctttat caatgtctcg aaatatccgc
     6601 cctccctgct gtatatcctg atgacactgg gaccaggcct cctcttcctc tccgtcgccg
     6661 aacgccccct caacaagatc accgcaccga ttgccgtctt cgggcgcgta cccttcttct
     6721 actatatcct gcatatctac ctcttccacg ttatcgccgt cctcgccgcc atggcccagg
     6781 gtcaccccgc atcacagatg accaacctca ccaactgggt aaccaataac ccacaattga
     6841 aaggctatgg cttcgatctc tggggcgtct acctcgcctg gatcgtcgtc gtcctggccc
     6901 tctatcccct ctgcaaacgt ttcgataagt acaaacgcag ccaccaggca caaaaaaaat
     6961 ggctgagcta cctctagcca gccaacaccc gggcgcttgc aacctcgcgg cgccccacaa
     7021 acgatcatcc ctcaccaaca cccggcgccc gcaaacctcc tcaccaacac ccgggcgccc
     7081 ccacaaacta ccgccccagc gcccgcacga tgcccggcaa ctcctccgtt gccgccacca
     7141 catccaccac tacccgccgc aacgcctgct gcggcatata cggcacctcc gccgtccgcg
     7201 ggtcctgcac aatcgtaaac ccgccccggc tcttcacata ctccatcccc tccgccccat
     7261 ccgcattccc acccgacaac aacaaggcaa taaccccctc cccatacaca tccgccgccg
     7321 accggaacgt cacatcgata ctcggccggc tgaaattcac ccgctccgaa taatccagcg
     7381 aaaaactccg gtcctgctcc agcaaaacat gataatccgc cggacagata taaacgatcc
     7441 ccggccgcaa cagctccttc tcctccacct ccttgatcac ctggtgcgtc cggctcgaca
     7501 acaactcctc caggctcgac tcgaaacccc cattccgatg caacaccacc aacaccggca
     7561 tggggtaatc cttccccaat gccgccaata acggcaggat cacctgcaga ctccccgcgg
     7621 agcccccaat gatcaataat ttatcctggc tcatgcacct tcctccatat cttttcccgc
     7681 cccacctgcc ggtagcggtc ggccatcggt gaaaaacgca gcgattcctt cgatcccaac
     7741 gcgagatacc cgaaattctc caggctgtaa tcgaacaacc cgaacacctt cgtctgcaac
     7801 tcccgctcga aatagatcag cacattccgg caaaggatca attgaaaggc attaaaagaa
     7861 aaatccgaca ccaggttatg cgtcgaaaag atcatcttcc ccgtcaacga cgaatcgaac
     7921 ttcgccaaat gatagttcgc cgtataatac gaagagaaat ccctcaaccc gcccgactgg
     7981 atataattct cggaatactt ctgcatctgg ctcaccggga agatcccctt ccgcgccttc
     8041 tcgatcacaa gagggttgat atccgttcca tacagcaacg acttatgcag caacccctcc
     8101 tccttcagca aaatggccat cgaatacacc tcctcccccg tggaacatcc cgcatgccag
     8161 atacggataa aaggataagt agccagtacc ggcaggatcg tcatccgcaa cgtccggtag
     8221 aacgaagggt cccgcaacat ctccgtcaca ttcaccgtta tctcctcgac aaaccgccgg
     8281 aaatactggc tgtcatgttg cagccggtac cggaactcgg caaacgacgg aaatttatcc
     8341 agcgcaaaaa gccgctgcac ccgccgctgt aacgaagcct tcgagtactc cgtaaaatca
     8401 tatccatatc gggtatagat atcattcaac agcagttgca gttcctcatt ctgtagttga
     8461 taaccttcca tcgattattt ctcgtatagc cagatacgca ataaagacag taactgatcg
     8521 atgtccacgg gcttggatat atagtcggaa gcgcccgcct ggatacaccg ctcccggtcc
     8581 cccgccatcg ccttggccgt gaccgcgatg atcggcagcc gccgcagtcg cgggttccgc
     8641 cggatccgcg tgatcgcctc gaaaccatcc atctcgggca tcatcatatc catcaggacg
     8701 atatccacgc ctcccccttc cgctacccct aacgcctcct tcccatccat cgccgtcacc
     8761 accttcatct tgtgctgctc gagcgccttg gtcagggaga agatattccg tacgtcatca
     8821 tcggccacca gcaccgattt atccctcaac acctgatcca gtaccccgag ccggccggcc
     8881 ccgttcccat tggccccggc ctccttcccc tcgctgacga catgcaggaa gagcgacacc
     8941 tcatccaaca cccgctgata agaatgcgcc gtcttgacga cgatggagtc cgcatactgc
     9001 cgcaacctcg cctcctccgc cttggaaata tttattcccg tgaaaacgat gatcgggata
     9061 ttctcgaatg ccgcgttctt ccgcaccatc tccagcgtat cctcctcctc ctttcccgcc
     9121 tcccggctga ggatcacccc atgcacaccc tccttttgca ggatatccgc actctgcccg
     9181 atcgtcgggg cgatctccat atgcaggttg tgcgtaccca ggaaa
//
LOCUS       DATGFG010000811         5051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_437594, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000811 DATGFG010000000
VERSION     DATGFG010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437594"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VKQ52_15305"
     CDS             complement(<1..927)
                     /locus_tag="VKQ52_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLZ88621.1"
                     /translation="MGDLQIRKQPKKYDAVIVGSGAGGGMAAYVLAHAGLKVCLLEAG
                     PMFDPKTDSNQLKNPWESPRRGASTKFRPFGDFDGCYWGWEVDGEPYTQTGNPGTIPW
                     EWWRARMLGGRTNHWGRISLRFGPKDFKRRSIDGLGDDWPIGYEDVKPYYDKIDKLLG
                     VFGTNEGLYNDPDGFFLPPPKPRLHELMIKKAAGTVGVPVIPSRLSILTKQINSERGQ
                     CFFCSQCGRSCKVYGDFSSSSCLVIPALKTGNVDLVTNAMAREVLTDKEGLATGVSYI
                     NKDDLQEYQVSGRTVILAASACESARLLLNSKS"
     gene            complement(1009..2697)
                     /locus_tag="VKQ52_15310"
     CDS             complement(1009..2697)
                     /locus_tag="VKQ52_15310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HLZ88622.1"
                     /translation="MTHFLFRAAFLLSIPCAILSCNPADKTASTGDAGLATFNKDSLA
                     AAIKTLASDEFQGRRPFTPGETKTIGYLVGAYTALGLEPGNGNSYTQDVPMVEISPSA
                     PSTLQFTSRKGAVALQNMTDFVCWTERPDTVNTLDKNDVVFAGFGIVAPEYNWNDYAG
                     LKVKGKTVVVMVNDPGFYDSTLFKGHTMTYYGRWTYKYEEAARQGASACLIIHNTAAA
                     SYPFSVVQSSNGGVKLHLDTRSNPGYQLTLQGWVTEPAAMRLLAAAGKDSSLLKQAQH
                     QGFRPLPLDLKLSGSVHAKEVYNRSHNVIAKITGSTFPNEYVIFSAHWDHLGIGKPDA
                     KGDSIYNGAADNASGTAALLQIARAFKALPEKPERTVIFLSVTAEEQGLLGSAWYAQH
                     PVYPLSTTVANLNIDVLNTYGPTKDITYSGKGQSDLEDYLAAEIKNNGRYVAPEDHPE
                     AGHYFRSDHFNFARAGVPSLTADGGVDDATRGIAYGKQKHDEYTAQRYHQPADEYDPN
                     TWNLEGGLADIRIVYAIGHHLADSHEWPQWKPGSEFKAIRDQSAADRSKTSQQQ"
     gene            complement(2755..3783)
                     /locus_tag="VKQ52_15315"
     CDS             complement(2755..3783)
                     /locus_tag="VKQ52_15315"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLZ88623.1"
                     /translation="MIRSFFLLLPFIAVLPVRGSDLTVTDTLPLPCMQKAEKLLDEAL
                     SFMQRTYYRRDEVSWPDLTTKARQQLLTAGNCDDAYASISWCFRQLNEHHSFVMPPDK
                     AARYNRDEESPAPEPPLSQLVGEIKGEWLQDSIGYLTVPWVSTSDSLICERIADSLQA
                     VIARLDARGISRWIIDLRKNSGGNCWPMLTGVGPLLGDGVYGYFVASGERIPLAYRDG
                     SSFQGRHVRCRVSNKGYHTQRDHKAIVVLTGRRTVSAGEIVALAARGREQTCLIGEPT
                     AGLTTANATYSLSDRSMLVLTVCQEADRTGRICEGSIQPDKLIAANTPAVGDDPARTA
                     AINWLLLQ"
     gene            complement(3849..>5051)
                     /locus_tag="VKQ52_15320"
     CDS             complement(3849..>5051)
                     /locus_tag="VKQ52_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ88624.1"
                     /translation="WEEVSSRIQDGLTGIKTFKLSGAENREVEQLQAKAGEAYKDYIV
                     RARLANKYVFWETMLSHLSTALVFGYGGYLTLQNRLTPGDVVMFVAYLDRLYGPIDTL
                     ASLWVELQQNIASMSRAFRLLDKGIEEEAGPELTIRQGKIEFRDVRFSYTAGREVLKG
                     LSFTLEPGTTTALVGGSGAGKTTTADLLMKLYDPTGGSILIDDQDLRTLDAASIRRQI
                     GVVSTDGAVFRGSLADNIRYKRPSATDAEVEEAAIAAGMHSTLERLPEGLQTPVGESG
                     MGLSVGERQRVQIARILVAQPRILVLDEATANLDYATEAEVRKTIEDLRKKTTIIIIA
                     HRYSMVRDADHVIVLADGKVEEEGTPAALLEKEGWFAGWAGTVEEEEIEEEEEDEDTG
                     EEEAEEEE"
BASE COUNT          966 a         1411 c         1533 g         1141 t
ORIGIN      
        1 cgattttgag ttgagcagga gcctcgcgct ttcgcaggcg ctggccgcaa ggatcaccgt
       61 cctgccgctt acctggtatt cctgcaggtc atccttattg atataggaca cgccggtggc
      121 gagcccttcc ttgtccgtga gcacttcgcg ggccatggcg ttggtcacga gatcgacatt
      181 accggtcttt agcgcgggga tcaccaggca ggaggaagag gagaagtctc catacacctt
      241 acaactgcgg ccgcactggg aacaaaagaa gcattggccg cgttcgctgt tgatctgttt
      301 ggtcaggatc gaaagccggg acggaataac gggtacgccg acggttcccg ctgctttctt
      361 gatcatgagt tcgtgcaggc gtggcttggg agggggaaga aaaaaaccgt cagggtcatt
      421 atagaggccc tcgttggtgc cgaacacccc aaggagttta tcgatcttgt cgtaataggg
      481 tttgacatcc tcgtagccga tgggccagtc atcgcccaaa ccgtcgatgc tgcgtctttt
      541 gaaatctttt ggaccaaacc gcagggagat gcggccccag tgattggtcc gtccgccaag
      601 catccgggcg cgccaccatt cccaggggat cgtgccggga ttaccggttt gtgtataggg
      661 ttctccatcg acttcccatc cccaatagca tccgtcaaag tcaccaaagg gcctgaattt
      721 ggtgctggcg ccgcgacggg gagattccca gggatttttt aattgattgc tatcggtctt
      781 cgggtcgaac atcggacctg cttccagcag gcatactttg agccccgcgt gggcgagcac
      841 ataggcggcc atgccgccgc cggcgcccga gcctacgatc actgcgtcgt atttcttggg
      901 ttgttttctg atctggaggt ctcccattgt tttcgggtgg tagttaatag ataaagggta
      961 aatttaagcg tttagcaaaa cagcaaacaa aaatatttta cctgtggttt attgctgttg
     1021 tgatgtcttg ctcctgtccg cagccgactg atcacggatg gctttgaact cggaaccggg
     1081 tttccactgc ggccactcgt gactatcggc gagatgatgc ccgatcgcat acacgatcct
     1141 gatgtccgcc agaccgcctt cgaggttcca ggtgttggga tcgtattcgt cggcgggttg
     1201 atgatagcgc tgcgcggtgt actcgtcgtg tttttgtttg ccataggcga tacccctggt
     1261 cgcgtcgtcg acgccgccat cggcggtgag ggaagggaca ccggcgcggg caaagttgaa
     1321 gtgatccgaa cggaaatagt gtccggcttc gggatgatcc tccggcgcga catagcggcc
     1381 gttgttcttt atttccgcgg ccagataatc ttccaggtcg gattgccctt tcccgctata
     1441 ggtgatatcc tttgtcggac cataggtatt gagaacgtcg atattcaggt tggccacggt
     1501 ggtggaaagc gggtacaccg ggtgttgtgc ataccaggct gagccgagca gcccctgttc
     1561 ctccgcggtc accgagagga agatgaccgt acgctccggt ttctccggca gggctttgaa
     1621 agcccgtgct atttgcagca gtgccgcggt gccgctggca ttgtccgccg cgccattata
     1681 gatggagtcg ccttttgcat cgggcttgcc gatgccgaga tgatcccagt gtgcggagaa
     1741 gatgacatat tcattgggaa aggtactgcc ggtgattttg gcgatgacat tatgcgatcg
     1801 gttgtatacc tcctttgcat gaacggagcc cgatagtttc agatcgagtg gaagggggcg
     1861 gaatccctgg tgctgtgcct gtttcagcag gctgctatcc ttgccggcag ctgcaagcag
     1921 gcgcatggcg gccggttcgg tgacccagcc ctggagggtc agctgatagc cggggttgct
     1981 gcgggtgtcg aggtgcagct tgacgccgcc attggagctt tgcacgacgc tgaaagggta
     2041 ggaggccgcc gcggtgttat ggatgatcag gcaggcgctc gcgccctggc gcgcggcttc
     2101 ctcgtattta tacgtccagc ggccataata ggtcatcgtg tggcctttga acagcgtcga
     2161 gtcataaaaa ccagggtcat tcaccatgac gacgaccgtc tttcccttca ccttcaaccc
     2221 cgcatagtcg ttccagttat attcgggggc cacgatgcca aagccggcaa agacgacatc
     2281 gttcttatcc agggtattca ccgtatcggg tcgttccgtc cagcaaacga agtccgtcat
     2341 attctgcaag gcgacggcac ctttccggga cgtgaattgc agggtggagg gagcggaggg
     2401 actgatctcc accatcggca cgtcctgggt atagctgttg ccgttgcccg gctccagtcc
     2461 cagggcagta taggccccga ccaggtaacc gatggtcttt gtttcgccgg gagtaaaggg
     2521 ccggcgtccc tgaaactcat cggaggcgag cgtcttgatg gcggccgcga gactgtcttt
     2581 gttgaaggtg gcgagtccgg catcgcccgt ggaggccgtc ttgtctgccg gattacagga
     2641 gaggatcgcg caggggatgc ttaaaaggaa ggccgcgcgg aaaaggaaat gggtcatgtg
     2701 ctgtgtttga tagaatctcc taaaattagg atattctttt ctgtcgccag cgggctattg
     2761 caacaggagc caattaatag ccgcggtcct ggccgggtca tcccccacgg cgggggtatt
     2821 ggcggcgatc agtttatcgg gttggatgct gccttcgcag atccggccgg tacggtcggc
     2881 ttcctggcag accgtcaaca ccagcatcga ccggtcggag agggagtagg tggcattggc
     2941 cgtggtcagg cccgcggtcg gctcgccgat aagacaggtc tgttccctgc ccctggcggc
     3001 gagggcgacg atctcgcccg cgctgacggt ccggcgaccg gtgagcacga cgatcgcctt
     3061 gtgatcgcgc tgggtgtgat aacccttatt gctcacgcgg cagcggacgt ggcggccctg
     3121 gaaggaggac ccgtcacgat aggcaagggg aatgcgttcc cccgaagcga cgaaatagcc
     3181 atatacccca tcgccgagaa ggggaccgac gccggtgagc atcggccagc agttgccgcc
     3241 ggagttcttc cgcagatcga tgatccaccg ggagatgccc cgggcgtcca ggcgggcgat
     3301 aacggcctga aggctgtcgg cgatgcgttc acagataagc gagtctgacg tgctgaccca
     3361 gggaacagtc aggtagccga tgctgtcctg cagccattcg ccttttattt ctcctaccag
     3421 ctgcgaaagc gggggctccg gggcggggct ttcttcatcc ctgttgtacc gggccgcttt
     3481 gtccggcggc atgacaaaac tgtggtgctc gttcaactgg cggaaacacc aactgatcga
     3541 ggcataggca tcatcgcagt taccggcggt gagcaactgt tgtctggcct ttgtggtcag
     3601 atcgggccag gacacctcat ccctccggta atacgttcgc tgcatgaagc tgagggcttc
     3661 atcgagcagc ttttcggctt tttgcatgca gggcaatggc agcgtatcgg taacggtcag
     3721 gtcactgccc cggacgggga ggacggcgat gaacggcagt aagaggaaaa aggatctgat
     3781 catgggtagt cgaggttgga tcatcccata agatacgcaa aaggaagaat tccataacaa
     3841 agtgagcgct attcttcctc ttccgcttct tcctccccgg tgtcctcgtc ttcttcctct
     3901 tcttctatct cttcctcctc caccgttccg gcccagccgg cgaaccagcc ttccttttcc
     3961 aggagtgccg ccggtgtgcc ttcttcttcg actttcccat cggccagtac gatgacatgg
     4021 tcggcatccc ggaccatgga ataacggtga gcaataatga tgatcgtcgt ctttttacgg
     4081 aggtcttcga tggttttcct gacttccgct tcggtggcat agtccaggtt ggccgtggct
     4141 tcatccagca cgaggatccg gggctgtgcc accagtatcc tggcaatctg gacacgttgc
     4201 cgttcgccga ccgacagccc catgccgctc tcccccaccg gcgtctgcaa tccctccggc
     4261 aggcgttcca gcgtggagtg catgcccgcg gctattgccg cctcttccac ctcggcatcg
     4321 gtggcggaag ggcgtttata acggatatta tcggccaggc taccccgaaa gaccgcaccg
     4381 tccgtagaca ctacaccgat ctgccgccgg atggatgccg cgtcgagggt gcggaggtcc
     4441 tgatcgtcga taagaatgct cccgccggtg ggatcataga gtttcatgag cagatcggca
     4501 gtggtcgtct tgcccgcacc ggagccacct accagggcgg tcgtcgtacc gggctccagg
     4561 gtgaacgaca ggcccttcag tacttccctc ccggcggtat aactgaaacg gacatcgcgg
     4621 aattcgatct ttccctgccg gatcgtcaat tccggaccgg cctcttcttc gatgcctttg
     4681 tccagcagcc ggaaggcgcg ggacatggac gcgatgttct gctgtagttc gacccaaaga
     4741 ctggccaggg tatcgatggg cccgtagagc cggtcgagat aggccacgaa catgaccaca
     4801 tcgccggggg tgagccggtt ctgtagggtc aggtaacctc catagccaaa caccagggcc
     4861 gtggaaagat gggacagcat ggtttcccag aaaacgtatt tattggccag ccgcgcccgc
     4921 acgatataat ccttataggc ctcgccggcc tttgcctgca gctgctccac ttcccgattt
     4981 tcggccccgg agagtttgaa ggtcttgata cccgtgagtc catcctggat gcgcgaagag
     5041 acctcttccc a
//
LOCUS       DATGFG010000812         5400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1273585, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000812 DATGFG010000000
VERSION     DATGFG010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273585"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3066)
                     /locus_tag="VKQ52_15325"
     CDS             complement(<1..3066)
                     /locus_tag="VKQ52_15325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ88625.1"
                     /translation="MKKLLMTTGLLVLFCLTLPGPLSAQQAKGGGEPHFKGKITNEKG
                     EPLAGASVRHKNSTRGTTTDAGGEFNLSAKPGDLIVISYVGYIEVQHVVAGGEDIHVA
                     LKPVAGSNMNDAIVIGYGTQKKANVLGSVSVVKAEDLVVTKNENVVNMLTGKVPGLRI
                     QQMSSEPGSFNTQYDIRGFGSSSTGNPTPPLIIIDGVPRGSGDLARMDPSEIDNISVL
                     KDASAAIYGVKAANGVILVTTKRGSRNGKFNITYSVNQSWQQFLDVPNPVNAVQYMQL
                     WNENAQRNFGNNVTGTAPKAYSDSTIALYTSGKLKSTNWMKAITRPLAPETQHNLNMS
                     GGTDKVNYFFDLGYLTQGSLFKTNSINYDRWNFRSNVNINFTSRLRGAALVSGYSDTK
                     NAPNSAVWTIFKYAENLVPTDQPYANNNPKYPSLEPDNANPVVMTNSDLVGQNIYKNK
                     NFQGQLNLEYDIPGITGLMAKAMYNYGYSVADNTIVNKSFNLYTYDQGTQTYKATLVH
                     SPSTVQRQYYTNTNTLMQLSLNYKHQFGGVHNVTGLVLYEEQYNTGDDFQAQESFSLG
                     IPYLFAGNAGDVNYRTVGQDKGNLINLVTRSVVGRFDYDYKGRYLGEFSFREDASSKF
                     SPVKRWGFFPAGLIGWRISNEPFMQALIPPGVLNNLKIRASYGVLGDDQAQNYQWIAG
                     YNYPGPGAVINGSYVSGINALGIPNLALTWMQSKTLDIGADFDLWNGLIGGSFDYFRR
                     NRTGIPDLPNAQVPGTSGLVVNQANLDGDHTYGVELVLTHRHTIRKVAYTISGNIGIS
                     RIRQDFKEEVRANSAYDQYKNELSGRYTNIWWGYLYGGQYTNYNQIYGSNVNTGGGNQ
                     SVVPGDYYYQDINHDGVIDAKDQVPIATRDLPIINFGLTLGASWNSFDLSMLLQGATD
                     YHVQFAEQLAAPLQYQRSALIEFMNRWHTADPNANMFDPNTVWVPGNYPTTGSPAADG
                     SKAVQNATYMRIKTLEIGYTLPKKLLNSIGIQNIRVYVNSYNLATLTGLK"
     gene            3664..>5400
                     /locus_tag="VKQ52_15330"
     CDS             3664..>5400
                     /locus_tag="VKQ52_15330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88626.1"
                     /translation="MFKNYLKTAWRNLMNDRIHAVINVSGLAIGIAVALLIGLWIWDE
                     LGFDTYNKNYKTIAQIARKEISKGDVYISAGSNHFPIPLADELRKNYTNVFRHVSLTS
                     ERSSHLVGYNDSRLIASGIYVEKDFTDIFTLKLIAGSAAGFSDPNTVFVGQSVAKALF
                     GETDPVGKIVQLDHTQPLKVIGVFDDFPLNTSFSGVGLICPFNLLASTNQGVKAILND
                     WNNSSFFIYTDLQPGLSGSRVSEAIKEAYWSKIKNSTAQPPGNKVELFTHPMKDWHLR
                     SEWKAGVPSGGQIQIVRLFSLIGVFVLLLACINFMNLSTARSEKRAKEVGVRKTMGSR
                     RIGLIRQFLGEALLTVLLAFGIGVGIVILSLHWFNEIAQTNITLPFSNGWFWLFAAAF
                     ILLTALVSGSYPALYLSSFKPVQVLKGSFKAGKYTTLPRKALLAIQFVVSIVLIIGTI
                     VVYRQIQLARDRPLGYNRNGLIRITMTTPDLKGKYDVLRQELLSSGGAIGFAESSSAA
                     TENNYYDDHFEWEGKNLKTHGQSFALTAVTPEYAGTVGWQFEEGRNFSRRFGTDQSAL
                     ILNEAAVKYMGLS"
BASE COUNT         1230 a         1367 c         1418 g         1385 t
ORIGIN      
        1 cttaagaccg gtgagggtgg caagattata gctgttgaca tagacacgga tattctggat
       61 accaatgctg ttcagcagtt ttttcggtaa agtatatcct atctccaggg tttttatgcg
      121 catataggta gcgttctgca cagccttcga gccatccgcg gcgggcgatc cggtagtggg
      181 ataattcccg ggaacccaga ccgtattggg atcgaacata ttggcattgg ggtcggcggt
      241 atgccacctg ttcataaatt cgatcagggc gctccgctga tattgcagag gagccgccaa
      301 ttgctcggca aactgcacat ggtaatcggt agccccctgt aacagcatgg agagatcgaa
      361 actattccag ctggcgccca gggtcaggcc gaagttgata atgggcaggt ccctcgtggc
      421 aatgggcacc tggtccttgg catcgatgac gccgtcatgg ttgatgtcct ggtagtaata
      481 gtcaccgggt acgacggatt ggttaccgcc gcccgtgttc acattcgaac catagatctg
      541 gttgtagttg gtatattgcc ctccatacag gtatccccac cagatattcg tataacggcc
      601 ggacagttca ttcttgtatt gatcatatgc actgttggcg cggacttctt ccttgaagtc
      661 ctgcctgatc ctgctgatac cgatattgcc gctgatggta taggccacct ttcggatagt
      721 atgacggtgc gtaagcacaa gttcgacgcc ataggtatgg tcaccgtcaa gattcgcctg
      781 gttgaccact aaacccgagg ttccgggaac ctgggcattc ggaagatcgg gtatacccgt
      841 ccggttcctt ctgaaataat cgaagctgcc gccgatgagc ccgttccaca ggtcgaagtc
      901 cgccccgata tcgagggtct tggattgcat ccaggtcagc gcaagattgg gtatgcccag
      961 ggcattgata ccactgacat aactaccgtt gatcacagcg ccggggccgg ggtagttata
     1021 acccgctatc cactggtaat tctgggcctg gtcgtccccc agcacaccat aagaggcgcg
     1081 tatcttcagg ttgttcagga cacccggcgg gatcagggcc tgcataaagg gttcgttgct
     1141 gatgcgccag ccgatcaggc cggcagggaa aaatccccag cgcttcacgg ggctgaattt
     1201 actggaagca tcctcccgga aagagaattc ccccagatac cgtcccttat agtcatagtc
     1261 gaaccgaccg accacgctcc tggtcaccag gttgataaga ttgcctttgt cctggcctac
     1321 ggtgcggtag ttgacgtcac ctgcattacc cgcaaaaaga taggggatgc ccagggaaaa
     1381 gctttcctgg gcctggaaat catccccggt gttatattgt tcttcataca ataccagccc
     1441 cgtaacgtta tggacgccgc caaactgatg tttataattg agggacagct gcatcagggt
     1501 attggtattg gtataatact ggcgctgcac agtggagggt gaatggacca atgttgcctt
     1561 gtaggtctgg gttccctggt cataagtata aagattaaag gacttattga caatggtatt
     1621 gtctgccacg ctgtacccat agttgtacat ggcctttgcc atcaggccgg taatgccggg
     1681 aatatcgtat tccaggttca gctggccctg gaagttctta ttcttataaa tattctgacc
     1741 taccaggtcg gaattggtca tgacgaccgg gttggcgtta tccggctcca ggctgggata
     1801 tttcgggttg ttgttggcat agggctggtc ggtaggcacc aggttctcgg catatttgaa
     1861 tatggtccat acggccgaat tgggggcgtt tttggtgtcc gaatatcccg ataccagtgc
     1921 cgcacccctc aaccggctgg tgaaattgat attcacattg ctccggaaat tccaccggtc
     1981 gtaattgatg ctgtttgtct tgaacaggct gccctgggta aggtagccca ggtcgaaaaa
     2041 gtaattcacc ttgtcagtgc cgccggacat gttcagatta tgctgcgtct ccggggccag
     2101 gggccgggtg atggccttca tccagttggt ggatttcagc ttgccgctgg tatataaagc
     2161 gatcgtacta tccgaatagg ccttcggggc ggtaccggtc acattattcc cgaaattccg
     2221 ctgcgcgttc tcattccaca gctgcatata ctgtacggca ttgacgggat tggggacatc
     2281 caggaattgc tgccagctct ggttgacgga ataagtgata ttgaatttac cgttccggct
     2341 gccgcgcttt gtggtcacca ggatcacgcc gttggcagcc ttcaccccgt agatggcggc
     2401 ggaggcgtct ttcaataccg atatattgtc aatttcggaa gggtccatcc gggcaaggtc
     2461 gccgctgccg cggggcaccc catcgatgat gatcagcggc ggggtgggat tgccggtgga
     2521 actactgccg aatccacgga tgtcgtactg ggtgttgaaa gacccaggct cggagctcat
     2581 ttgttgtata cgcaggcccg gcacttttcc cgtcagcata ttgaccacat tctcgttctt
     2641 ggtcactacc aggtcttccg ccttgaccac cgatacggag ccgaggacat tcgccttttt
     2701 ctgcgtacca taccctatga cgatcgcgtc gttcatgttg ctgccggcga caggcttcag
     2761 cgctacatgg atgtcttcac ctccggccac gacatgctgc acttcaatat atccgacata
     2821 gctgatcacg atcaaatccc cgggctttgc cgaaaggttg aattcaccgc cggcatccgt
     2881 cgtggtcccc ctggtgctgt tcttgtgccg gacactggcg ccggccaggg gttccccctt
     2941 ttcattcgtt atcttccctt taaagtgagg ttcgccgccc ccttttgctt gctgggcgct
     3001 cagcggcccc ggcagagtca aacaaaaaag gacgagcagc cccgtcgtca ttagcagttt
     3061 tttcatatat gtaagctttg ggtttaaaat cgtatatgga ataggagatc cctcggttac
     3121 cgaaggcaat cgttgcgaaa agagaaagaa taaataatgg tgtcaggaaa aagagatcat
     3181 ccggatggag gagggaagac aaataaataa gaagacagca gtctggccgc ggtagaatat
     3241 ttcatatgac aacgtttatg taactccttg taagaaagga gttatcggtt aattttctta
     3301 ttaactttcc cttaacagtg tcattaattc atattttttt ttacgcagcc ggagcgtccc
     3361 acgctcgccg ctgcaaccac ggcggattat ctccggcgcc ctcccgccat ctccggcgcc
     3421 ctcccgccgc cttcgctgat cccgataacc ctgcgagggc cttacccaaa gccctcccgt
     3481 cccctcagcg gtgatgcaat tttcctggat catttaggta cgatacatgt gcggaagcga
     3541 acatattaca attggttaaa tttttattgc catgattaac ttagttttag gaactggcat
     3601 ctcacttgcg ctttacttcg gcggacgcga taaagaggct ccaatcaatc aacaatattg
     3661 tctatgttca aaaattattt gaagaccgcc tggcgaaatc ttatgaatga caggattcat
     3721 gcggttataa atgtaagcgg tcttgctatc ggtattgccg tggcgctctt aatcggattg
     3781 tggatctggg atgaacttgg ctttgatacc tacaacaaga actataagac catcgctcag
     3841 atcgcgagga aggaaatttc gaaaggcgat gtctatattt cagccgggag caatcatttt
     3901 cccataccgc tggcggatga gcttagaaag aattatacta atgtattccg gcatgtctcg
     3961 cttacttccg aaagaagcag tcacttggtg ggctacaacg atagccggct tattgcgtcg
     4021 ggcatttatg tggaaaagga tttcaccgat atttttacgt tgaaactgat cgccgggtct
     4081 gccgcggggt tttccgaccc taataccgtt ttcgttggcc aatccgttgc caaagcgctg
     4141 tttggggaaa cagacccggt tgggaagatt gtacaactcg atcataccca gccgttaaaa
     4201 gtgattggcg tatttgacga cttcccgctt aacacatcgt tttcgggggt cgggcttatt
     4261 tgccccttca atctgcttgc cagtacgaat cagggagtga aggccatcct gaacgattgg
     4321 aataacagtt ccttttttat ttataccgac ctgcaacccg gcctttcggg cagcagggta
     4381 tccgaggcca ttaaagaggc ctactggtcg aaaatcaaga atagcaccgc tcagcccccg
     4441 ggtaataaag tcgaattgtt cacgcatccg atgaaagatt ggcatcttcg atccgaatgg
     4501 aaggcgggtg tacccagcgg cggccagatc cagatcgtcc ggctatttag cctgatcggc
     4561 gttttcgtgt tattgctggc ctgtatcaat tttatgaatc tcagcacggc gcgttcggaa
     4621 aaaagggcta aggaggtggg tgtccggaaa acaatgggct cgcggcggat cgggttgatc
     4681 aggcaattcc tgggcgaagc gttactgaca gtgctgcttg cattcggcat cggcgtcggt
     4741 atcgtgatcc tgagcctcca ttggttcaat gaaatagccc aaaccaacat caccttacct
     4801 ttttcaaacg ggtggttctg gttgtttgct gcggccttca ttctcctgac cgccctggtc
     4861 tccggcagct atcctgcctt gtatctttct tccttcaagc cggtacaagt attgaaaggc
     4921 agcttcaaag cgggcaaata cacaaccctg ccgcgaaaag cgctgctggc catacagttt
     4981 gtcgtgtcca ttgtactgat catcgggacc atcgtcgtat atcgacaaat ccagttggcc
     5041 agggaccgcc cgctcggcta caacagaaat ggcctgatcc gcattaccat gactacgccg
     5101 gatctgaaag ggaaatacga tgtcctgcgc caggagcttt tatcatcggg cggcgcgatc
     5161 ggtttcgcgg aatcttccag cgctgcgaca gagaacaatt attacgatga ccattttgag
     5221 tgggaaggca agaacctaaa gacacatggc cagtcgtttg ccctgacggc agttactcct
     5281 gaatatgccg gaacagtagg ctggcaattt gaggaaggac gcaatttctc gcggagattc
     5341 ggcaccgacc aatcagcctt gatcctcaat gaagcggcgg taaaatatat gggtctttcg
//
LOCUS       DATGFG010000813         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_880174, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000813 DATGFG010000000
VERSION     DATGFG010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880174"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..675
                     /locus_tag="VKQ52_15335"
     CDS             112..675
                     /locus_tag="VKQ52_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLZ88627.1"
                     /translation="MDYHQLVKDCLRGDTAAQKLLYEHFAGGMLGVCYRYTKSMVDAE
                     DVLQEGFIMVFRNLHQFSFTGELGGWIRRIMVNTAINYLKKHSRYQTDLLFTEGNLHP
                     VSDDDPEVSLSTKELAELIRQLPPGYQAIFNLHAIEGYSHVEIGKILGIKEGTSRSQY
                     ARARSLLISWIYKYSATNKNESNYVRP"
     gene            662..2191
                     /locus_tag="VKQ52_15340"
     CDS             662..2191
                     /locus_tag="VKQ52_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88628.1"
                     /translation="MYDPKFEKGVQQKMEGLQFIPSESVWANIEKAVANRRRRRTAFY
                     FWRVAIPAGIMLAGATALLYHPSRPVAGLSPATKPFPAAPSAATPVTASNGATTKTMT
                     PASTAHQPATAFEAATAHTVTPASILPSTASTAAPTRSARSHLRSSHPGTPATGGTTP
                     TEPAENATTNRTSAGKTPATTDITAISATGKTAHTANTPATGNTAADANTPAGTNTPG
                     LANTTRTRFSYRPTLAGLRHASSIQAAKLSTKRTIIGLSAIQKTQRPWEAGFVGGGGI
                     SRLNRLNVTQPMSSAAASFVNITTAAYTATAGTNKNYVSDVRPGASFLAGIYLQKAFS
                     SRWTINLGMNLHYYSTMISVGEPVSTYVPLTVSLIAPTAQASSQATTAYTAGNRNVVT
                     NRYYFMELPVNIQYTLNKSRILPLFLQGGFSLSRLMASDALFYNSHSGVYYRDASVLQ
                     KTQFNVSSALMAGLPFHGIRIQVGPEIQYGLTPLINNKSLGDQHFLYTGIRLVVIPGR
                     R"
     gene            complement(2215..>2938)
                     /gene="queG"
                     /locus_tag="VKQ52_15345"
     CDS             complement(2215..>2938)
                     /gene="queG"
                     /locus_tag="VKQ52_15345"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HLZ88629.1"
                     /translation="RSVITLLMNYYPPVLQEPAAPQVAKYAYGKDYHEVIRSKLNELL
                     VRMRSVIGEVDGRGFVDSAPVLERSWAVRSGLGWIGRNGNLLNRQAGSFFFIATLIVD
                     LGLEYDAPFSGDYCGTCRRCIDACPTEAIREDKVVDGSRCISYFTIELKEELIPDEMK
                     GKFANWMFGCDTCQDVCPWNRFSRPSEEIAFTPMAAILNFKTADWEQLTEEKFREIFR
                     HSAIRRTRYAGLRRNLRFLEGE"
BASE COUNT          681 a          934 c          744 g          579 t
ORIGIN      
        1 tcaatgaagt tgtcatggcg gcaaagatag gtattaccga aaaaaggaga tgtcggggcc
       61 ccggtgcaac gaagcagcca atttgtttgt catcaagcgc aacaacggaa catggattat
      121 catcagttgg taaaagactg cctaagaggg gatacggctg cccaaaagct gctgtatgag
      181 catttcgcgg gtggaatgct gggggtctgt tatcgctata cgaaatccat ggtcgacgcg
      241 gaagatgtct tacaggaagg gtttatcatg gttttcagga atttgcacca gttcagcttc
      301 accggtgagc tgggtggttg gatccgccgg atcatggtca acaccgccat caattacctt
      361 aaaaaacaca gccgctacca gaccgatctg ttgttcacgg aagggaacct ccatccggtg
      421 tccgacgacg accccgaggt atcccttagc acaaaggagc tggcggagtt gatccggcag
      481 ctgccgccgg gctaccaggc tattttcaac ctacatgcca tagaagggta tagtcatgtg
      541 gaaataggga agattttagg tatcaaggaa ggcacctccc gttcgcagta tgcgcgggcc
      601 aggtccttgc tgataagctg gatatacaag tattcagcca ctaacaaaaa tgaatcgaac
      661 tatgtacgac cctaaattcg aaaaaggagt gcagcaaaag atggagggat tgcagttcat
      721 cccttccgag tcggtgtggg caaacataga aaaggctgtt gccaaccgcc gccgccggcg
      781 gactgcattc tatttctggc gcgtcgctat cccggcgggt atcatgctgg ccggagcaac
      841 ggccctgctg tatcatccgt cccggcctgt tgccggcctg tcaccggcga caaaaccgtt
      901 ccctgcggct ccttccgccg ccactcccgt cacggcttca aacggcgcaa ccaccaagac
      961 gatgaccccg gcatccactg cacatcaacc ggcaacggct tttgaggctg ccacggctca
     1021 tacggtaacc ccggcatcga tcctgccatc cacagcatcc accgcggctc ccacgagatc
     1081 cgcgagatca catctgcgct cctcccatcc ggggactccg gccaccggcg gaaccactcc
     1141 aaccgaaccg gccgaaaacg ccaccacgaa caggacctct gccggcaaga ccccggcaac
     1201 tacggatatc accgccattt ccgccaccgg gaaaaccgca cacaccgcga atacccccgc
     1261 caccgggaac accgcggccg acgcgaatac tcccgccggc acaaacacgc cgggcctcgc
     1321 gaacaccacg cgcacccgtt tttcctatcg accgacgctg gcaggcctgc gccacgcctc
     1381 ttccatccag gcggcaaaac tgtccacgaa gaggaccatc atcggcctca gcgcgatcca
     1441 aaagacgcaa agaccctggg aggcaggatt tgtcggcggc gggggcatct cccggctcaa
     1501 ccggctcaat gtgacacagc cgatgagttc ggccgctgcc tcctttgtca acatcaccac
     1561 cgccgcctat actgcgacag caggcacgaa taagaattat gtctccgatg tacgccccgg
     1621 cgcttcattc ctggcaggca tctatttgca aaaagcattc tccagccggt ggaccatcaa
     1681 cctcgggatg aacctgcact attattctac gatgatcagc gtcggcgaac ccgtgagcac
     1741 ctatgtaccc ctgacggtct cgctgatcgc gcccaccgcc caggcgtcct cacaggctac
     1801 cacggcctat accgccggca accggaatgt cgttaccaat cggtattatt tcatggagct
     1861 gcccgtgaac atccaataca cgctcaataa aagccggata ttgcctctct tcctgcaagg
     1921 cggcttctcc ctttcccgcc tgatggcctc cgatgcattg ttctataatt cgcactccgg
     1981 cgtttattac cgcgacgcca gtgtgctgca aaagacgcaa ttcaatgttt cctccgccct
     2041 gatggccggt ctgccctttc acggtatccg catccaggtg ggaccggaaa tacaatatgg
     2101 gctgaccccc ctgatcaata acaagagcct cggcgaccag catttcctct ataccggcat
     2161 ccgcctggtc gtcataccgg gcaggcggta gggcgaagga tgtaagggcg ccactcattc
     2221 cccctccagg aaacggaggt tgcgacgcag cccggcataa cgggtgcgcc tgatcgccga
     2281 atgacggaag atctccctga acttctcctc cgtcaactgc tcccaatccg cggtcttgaa
     2341 attgaggatc gcggccatcg gtgtaaaggc tatctcttcc gaaggccggg agaagcggtt
     2401 ccagggacaa acatcctggc aggtgtcgca gccaaacatc cagttcgcga acttcccctt
     2461 catctcatcg gggatcagct cttctttcaa ttcgatggta aaataggaga tacacctgct
     2521 gccgtccacc accttgtctt cccggatggc ttccgtcgga caggcatcga tacagcggcg
     2581 acaggtgccg caatagtccc cggagaaagg cgcatcgtat tccagcccga gatcgacgat
     2641 cagcgtggcg ataaaaaaga aggaacccgc ctgccggttc aacagattcc cattcctgcc
     2701 gatccagccc agtccgcttc ttacggccca gctccgctcc agcaccgggg cgctgtcgac
     2761 aaatccgcgg ccatccacct ccccgatgac cgatcgcatc ctgaccagca gttcattcaa
     2821 tttactcctg atcacctcat gataatcttt cccgtaggca tacttggcca cttgtggcgc
     2881 tgccggctcc tgcagtacag gcggataata attcatcagc agggtgatca ccgaacgg
//
LOCUS       DATGFG010000814         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_152662, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000814 DATGFG010000000
VERSION     DATGFG010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152662"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(368..1585)
                     /locus_tag="VKQ52_15350"
     CDS             complement(368..1585)
                     /locus_tag="VKQ52_15350"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HLZ88630.1"
                     /translation="MAGHTRIDELIVKKLLGEITIEEEIELQVLGKASEENRELIARM
                     SPEAFQRKWDALRSIDDQKLDRKMKQRMGHIPGVLEWVPETTWMVRAARPWSIAASIL
                     LLASVVWWFSHTFLTSKTISHQPIEAKLWSQSDTIELGKMEEGKAYEAGQIRIARMRN
                     QFFIHQWMDSAAVAANAIEYHLTVTGREKIQVFFQDSTRAQIYPGSGISFRVSPAVTL
                     LKDKEMACNGQVLFDVNPHYETPFIVKTPKQVVTVLGTLFAVRDYSKEDTGAVFCYTG
                     KVSVKDDGLAPQTLVAFQRVTVDPKHALQVSTGDFPQAEWSSPELLFDFSNLNLDSAM
                     NEIAHWYGLRKVKFAAGIDIKTPGTVLTGKLSRYLPLQQLLSILERNDLHFSIQGQTI
                     LVAGNGDRTSHDH"
     gene            complement(1684..2256)
                     /locus_tag="VKQ52_15355"
     CDS             complement(1684..2256)
                     /locus_tag="VKQ52_15355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ88631.1"
                     /translation="MTDTIDLFGQFLRGEPKGINSIVLAYRDPLISYGLKWVSRREDA
                     VDAVMEAFAILQEKIGQFESEIHIKKFLHRTVKNKCLAAGREGKRYATLPGNVENSAD
                     ISASEMIDIRESDAYSQWIIQKVQARLEQLPQKRRQDFHAHFFDSKSFKDIAGERGVS
                     VDTVRQNVEYAVKEIRQYLYEKGLPGLKKG"
     gene            complement(2500..3657)
                     /locus_tag="VKQ52_15360"
     CDS             complement(2500..3657)
                     /locus_tag="VKQ52_15360"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HLZ88632.1"
                     /translation="MQRFLLPMLLILSGSICAQSSFNGVESNLSNLYRLSPAKSRSIS
                     PENFNGAKGSGGKATNGTGSGAASDLGQGWKISPSVDIKAGQTFTIAEIEGPGSIQHI
                     WMTPTGNWRYSILRFYWDDEATPSVEVPVGDFFGMGWGQYAPLQSLAVCVNPGSAFNC
                     YWPMPFRKKCRITMENIDHKDMTVYYQVDYLETEIPKDAAYFHAQFRRVNPLPYKQNY
                     VLVDSIQGKGQYVGTYLAIGVHNNGWWGEGEIKFYMDGDTEFPTICGTGTEDYFCGSY
                     DFDTRQKTEHGNRESQYTEFCSPYTGLPQVIRGDGHYNVSQRFGLYRWHISDPIRFEK
                     SLKVTIQDLGWRNDGRYLPLQDDMASTVFWYQAEPHGVFPKLPAQDQLEIN"
     gene            3973..4185
                     /locus_tag="VKQ52_15365"
     CDS             3973..4185
                     /locus_tag="VKQ52_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88633.1"
                     /translation="MSSVTDQYQPAEKDLLFTQSLLQDLADYREARLVIQTRSPLVTR
                     DSALFRRFRAMQIDMTITTILVGRLF"
BASE COUNT         1016 a         1093 c         1043 g         1140 t
ORIGIN      
        1 aatttatcgt attcgaagac aagcccctgc ttttttagtg gctcgaagaa ttgttcgaag
       61 gtgattcgac catgagcgca acaaacgcca tccagctcat gcaaataggg ttggcagctt
      121 atgagaacct gcagctgatt cccgatgata cgaagaggga taatacaagc aaacggcagc
      181 tcctcgttgt gaccttggag ctacaaggac atagtctcgc catgagaaga taattttggt
      241 tgaacctact tccgcctccg gacctggaga cttgaatagg ctaaagaata gcgaggataa
      301 cgtttaatac aggctcccgc agtcgcccgg gatgaatatg ccgggccagg gagcaaggaa
      361 ggagggttta atgatcatgg ctagtgcgat ccccgtttcc tgccaccagg atggtttgtc
      421 cctggatcga aaaatgcaga tcgttacgtt cgagaatgga aagtagctgt tgcaggggta
      481 gatatcggct gagcttgccg gttaataccg ttcctggtgt ttttatgtct atccctgccg
      541 caaatttgac tttcctgagg ccataccagt gggcgatctc gttcatagcg ctgtcgagat
      601 tcagattcga gaagtcgaat aacagttccg gcgatgacca ttccgcttgt ggaaaatcgc
      661 cagtcgacac ctggagtgca tgctttggat caacggttac ccgttgaaaa gctactagtg
      721 tttggggcgc cagtccatca tctttgacgg aaaccttgcc tgtataacag aatacagcgc
      781 cggtgtcttc tttactataa tcacgcaccg cgaacagcgt ccctaatacg gtgaccacct
      841 gttttggagt ctttacgata aaaggagttt cgtagtgagg gttgacgtcg aaaaggacct
      901 gcccattgca ggccatttct ttgtccttta atagcgtgac ggcaggcgaa acccggaagg
      961 atatgcctga gccgggatat atctgcgccc gggtggaatc ctggaagaat acctggattt
     1021 tctcccttcc ggtgaccgtc aggtgatact caatggcgtt ggctgcaacg gcagccgaat
     1081 ccatccattg atggataaaa aattggtttc tcatcctggc aatccggatc tgcccggcct
     1141 cataggcctt cccttcctcc attttcccca gttcgatagt atccgattgc gaccatagct
     1201 tcgcttcgat cggttggtgc gagatcgttt tgctggttag gaatgtatgg gagaaccacc
     1261 agacaacgct ggccagcagc aggatggagg cggcaatact ccatgggcgg gcggctcgca
     1321 ccatccatgt cgtctccggc acccattcca acacgccggg aatatgaccc attctctgct
     1381 tcatcttcct atcgagcttt tgatcatcga tgcttcttaa ggcatcccat ttcctctgga
     1441 acgcttcggg gctcatccgg gctatgagtt cccggttctc ctcggatgcc ttccccagga
     1501 cctgcagttc gatctcctct tcgatggtaa tttcgccgag taacttcttc actatcaatt
     1561 catctattct tgtgtgaccg gccataacat gcttcgtttt ggattgatca taaattacta
     1621 ttttccatca gtctgaatcc gtcaatagta tattcagaaa gtgctatttt ccgagaaaaa
     1681 aaatcaacct ttttttagtc cggggagtcc tttttcgtac aggtattgtc gtatttcttt
     1741 taccgcatat tccacattct ggcgcacagt gtcgaccgag actccccttt ccccggcgat
     1801 gtccttaaaa ctcttcgaat caaaaaaatg agcgtgaaag tcctgtcttc ttttttgggg
     1861 taattgctcc aatcgggcct ggaccttttg gatgatccat tgactataag catcagattc
     1921 ccggatgtct atcatctctg aggcactgat gtcagcacta ttttcgacat tccccggaag
     1981 tgtggcgtat cgttttcctt ctctgcccgc agccaggcac ttattcttta ccgttctgtg
     2041 caggaatttt ttgatatgta tttcactctc gaactggccg attttctcct gcaggatcgc
     2101 aaaggcctcc atcaccgcgt ctacggcatc ctcccttctc gacacccatt taagtccgta
     2161 ggaaatcagc ggatcccggt aagcaagaac aatactatta attcccttcg gttcaccgcg
     2221 caggaattgt ccaaataaat ctatcgtatc cgtcataaga ttgcgttgtt ttaagtcata
     2281 catgcctgat aaagctgacg gattcaggct gatggcttaa acatcgatca atcacggtta
     2341 taattggagg aataacgata ttacgcaatg cgtaatcgaa tctttcagta tggggggtgc
     2401 gcagcaggtt gaatcatata aagggtaatt ttataagtat cgacgccgga cgttgccgat
     2461 taactaacaa tcgttaggat ttcttttcca caccctggat caatttatct ccaactgatc
     2521 ctgcgccggc aacttcggga acacgccatg cggttccgcc tggtaccaga agaccgtgga
     2581 cgccatatcg tcctgcaggg gcaaatagcg gccgtcgtta cgccatccga ggtcctggat
     2641 ggtcactttc agacttttct cgaagcggat aggatcggag atatgccagc ggtatagccc
     2701 gaaccgctgg gacacattat aatgtccatc tccgcggatc acctggggaa ggccggtata
     2761 cggggagcag aattctgtat actgcgattc tctgttgcca tgctctgtct tttgccgggt
     2821 gtcgaagtca tagctgccgc agaaataatc ctccgttccc gtgccgcaga tggttgggaa
     2881 ttcggtatcc ccatccatat aaaacttaat ctccccttcc ccccaccagc cattgttgtg
     2941 caccccaatg gcgaggtagg ttccgacgta ttgccccttg ccctggatac tgtctaccag
     3001 cacatagttc tgtttataag gtagcgggtt gacgcgccgg aactgcgcat ggaaataggc
     3061 cgcatctttg gggatctccg tctccaggta atccacctgg taatagacgg tcatgtcctt
     3121 atgatctata ttctccatgg taatccggca cttcttccgg aagggcatcg gccagtagca
     3181 gttgaaggcg ctaccagggt tcacgcagac agcgagggac tgcaagggag catactggcc
     3241 ccagcccatg ccaaagaagt cacccaccgg cacctcaacc gaaggcgtcg cctcatcatc
     3301 ccaataaaat cgcaggatcg aataccgcca attccccgtc ggcgtcatcc agatatgctg
     3361 gatggacccc gggccctcga tctcggcaat ggtaaaagtc tgccccgcct tgatatcgac
     3421 agacggacta atcttccacc cttggccaag gtcgctggcg gcgccggagc cggtgccgtt
     3481 ggtggctttg ccgccgctgc cttttgcccc gttgaagttc tcggggctga tgctgcgaga
     3541 tttggcaggc gacagccgat acaggttgga gagattggat tcgacaccgt tgaaggacga
     3601 ttgagcgcaa atcgagcccg acaggatcag gagcattggc aggagaaatc gttgcatgca
     3661 aaatggtttt gagttatcta atttaactat tttccgttag attttttgcg ccgttctgat
     3721 ctgcatcttg atatcggtcc tagtcccgtc cgtatttgca gaaagtagaa tgcaatcgtt
     3781 gcccctacat tatgactaca cattgaatcc ttattccggt tgttcgtttg gctgcaccta
     3841 ttgttatgct gcatttttct cccggcgtca agagcgaaag gatctttggg gattacgggt
     3901 ccatgtgaat gaaaatgcct tggaggagtt gaaaaaaagc gaaaatagcc cttgctcaat
     3961 aacaccattt acatgagcag cgtgacagat cagtatcagc ccgcggaaaa ggatcttctg
     4021 tttacccaat ccctcctaca agacctggcc gattatcgcg aagcgaggct tgtcatccag
     4081 acaaggagtc cattagtaac ccgggatagc gctctattcc gaagattccg ggccatgcaa
     4141 atcgatatga ccatcaccac gatactggtt gggcggcttt tttagacgga tgaaagctaa
     4201 aggcggcaag aagtatgcta ttgttgctac agctagaaag gcggctatta tctattataa
     4261 aatggtccgc ttcaaccctt tgataataag ga
//
LOCUS       DATGFG010000815         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_626282, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000815 DATGFG010000000
VERSION     DATGFG010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626282"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VKQ52_15370"
     CDS             complement(<1..199)
                     /locus_tag="VKQ52_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88634.1"
                     /translation="MFAVLNAYLFQHRSISIPGLGTIYLETLPAAVDVADRTMQPPAY
                     HFRFDKYFDAPDKEFFAFVAAQ"
     gene            complement(335..1990)
                     /locus_tag="VKQ52_15375"
     CDS             complement(335..1990)
                     /locus_tag="VKQ52_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88635.1"
                     /translation="MDKKIVLLVMFGLCYAGASLQAQDTTKRKTIDITSSFKPVLREA
                     VKINFNAAPPAVDTSRPRLSYSIPAQYLFLSYQPGEMKPVALQRDSINPWVNYNFIKI
                     GVGNIHIPYIRTGFSFGDGKTTFFNLFANQLSSKGSLPYQKNSLTDVKLMGTVKTDNN
                     LEWSGHVGFKNDVYHLYGFRPDTLKFTGDQLSQTFQTFYGGLSLRNTEPTEFGLLYHP
                     NIKISYFEDNHNPKGTEANTVLNLPLEKMIGKEFAFDLGFTADLTHYNLPSHTDAQNN
                     NIYYVNPAFLVKNSNFFLQAAVTPSWDNKKFYLLPNFLADISTDDKRFSIQAGFIGYY
                     DKGSYQRFESINPWLAQPTDSLRNTRMQEVYGGFKGSISNHFTYAAKIGFLTYKNMPL
                     FVNDSVGGGKDFLIRYESSMQALQLHGELSYLQGEDFTVTASLNINQYKDLHTEVKPW
                     GLLPVEFNTHLKWQAFKDFYAKLDLFAFTAAQYRSPTDGVAFKGDNGADMNAGVEFRI
                     TRMLNLWFQMNNIFNNKYERWHQYPVYGFNVLGGIIFSFGDKK"
     gene            complement(2015..>2455)
                     /locus_tag="VKQ52_15380"
     CDS             complement(2015..>2455)
                     /locus_tag="VKQ52_15380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ88636.1"
                     /translation="NQKGLSTDDKVLANMAIAKSYQANNQCETALQYFRTAAGLTKSA
                     YSAEARYQIADCLFHASQLKDAEKAAFEVINKSGSYEEWVTKAYLLLGDIYFAEKDFF
                     NAKATYQSIIENAKIEELRLQAQQKLAHVVEEEKKGSKIDSSSN"
BASE COUNT          609 a          688 c          545 g          613 t
ORIGIN      
        1 gctgagccgc aacaaaggcg aaaaactcct tgtccggggc atcaaaatac ttatcaaagc
       61 gaaaatgata ggcaggaggc tgcatggtcc tgtcagccac atcgacggca gccggcaggg
      121 tctccaggta gatcgttccc aatcccggga tgctgatgct tctatgttga aataaataag
      181 cgttcaggac cgcaaacata gtagtatcca ttttttgctt aagctgcgaa tctacttatt
      241 tttgggccat tcgggcaaag caaaatttcc ctccggccca aaaacatcta ataattggcc
      301 ttcggccgat gggcccgtgg cccatcctgt ctacttactt cttatcgcca aaagaaaata
      361 ttatcccccc cagcacatta aatccatata cggggtactg gtgccatcgc tcgtacttat
      421 tattgaatat attattcatc tggaaccaca aattcaacat ccgcgttatc cggaactcca
      481 cccccgcatt catatcggcc ccattgtccc ccttgaacgc cacgccatcg gtcggcgagc
      541 ggtactgggc cgccgtaaag gcaaaaagat ccagcttggc atagaaatcc ttgaaagcct
      601 gccatttcag gtgggtattg aactccaccg gcaacaggcc ccagggcttt acctcggtat
      661 gcagatcctt atattgattg atattcaggc tggccgttac cgtaaaatcc tccccctgga
      721 gataactcaa ttccccatgc agttgaagcg cctgcatcga cgactcatac cggataagaa
      781 aatccttgcc gccgcccaca ctgtcattga caaacaatgg catattctta tacgtcagga
      841 acccgatctt ggcggcatag gtgaaatgat tgctgatcga tcccttgaaa ccgccatata
      901 cttcctgcat ccgcgtattc cgcaggctgt ccgtcggctg cgccagccac ggattgatgg
      961 attcaaatcg ttgataggac cctttatcat aataaccgat aaagcccgcc tggatgctga
     1021 accgcttgtc atcggtcgaa atatccgcca ggaaattcgg cagcaggtaa aatttcttat
     1081 tatcccatga gggtgtcacc gctgcctgca ggaaaaaatt cgaattcttg accaggaagg
     1141 ccgggttcac ataatagata ttgttgttct gcgcatcggt atgcgaaggc agattgtaat
     1201 gcgtcagatc tgccgtaaaa cccaggtcga acgcgaactc cttcccgatc atcttctcca
     1261 gcggcaggtt cagcaccgta ttcgcctccg tccccttggg attgtggtta tcctcgaaat
     1321 acgagatctt gatattggga tgatataaca acccgaattc cgtcggctcc gtattgcgca
     1381 ggctaagccc gccataaaaa gtctggaagg tctggctcaa ctggtcaccc gtgaacttta
     1441 aggtatccgg ccggaagcca tagagatggt acacatcgtt cttgaacccc acatgaccgc
     1501 tccactccag gttattatcc gtcttgaccg tccccatcag cttcacatcc gtgagactgt
     1561 tcttctggta cggcaggctt cccttggaac tcaattgatt ggcaaaaaga ttgaaaaagg
     1621 tggtcttccc gtcgccaaag ctaaaacccg tacggatata cgggatatga atattgccca
     1681 cccctatctt gataaaattg taattcaccc agggattgat cgagtcccgc tgcagggcca
     1741 ccggtttcat ttctcccggc tgatagctga gaaaaagata ctgggccggt atcgaatacg
     1801 aaagccgggg acggctggta tccaccgccg ggggcgccgc attgaaattg atctttacgg
     1861 cctcccgcaa cacgggcttg aaggaagaag tgatgtcgat ggtcttgcgt ttggtcgtat
     1921 cctgcgcctg tagcgaagca ccggcataac acaggccaaa catcaccagt aaaacgattt
     1981 tcttatccat gtgaacgtac attccgggtt atcattaatt actactgcta tcgatcttgc
     2041 tgcccttctt ttcctcctct accacatgcg ccagcttctg ctgggcctgc aaccgtaact
     2101 cctcgatctt cgcattctct ataatactct gatacgtagc cttcgcattg aaaaaatcct
     2161 tctccgcaaa atagatatcc cccaacaaca ggtaggcctt cgtcacccat tcctcataag
     2221 accccgattt attgatcacc tcgaaggcgg ccttctccgc gtctttcaac tgactggcat
     2281 ggaaaaggca atcggctatc tggtaacggg cctccgcact ataggccgac ttggtcagcc
     2341 ccgcggccgt acggaaatat tgcaacgccg tctcacactg gttattcgcc tggtaggatt
     2401 tggcgatcgc catattcgcc aacaccttgt catccgtact aagccccttc tggtt
//
LOCUS       DATGFG010000816         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_187771, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000816 DATGFG010000000
VERSION     DATGFG010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187771"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VKQ52_15385"
     CDS             <1..1265
                     /locus_tag="VKQ52_15385"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HLZ88637.1"
                     /translation="MKIFKFGGASVNSVDRIRQIPGILRQYSGQKILVVISAMGKTTN
                     ALEKVAEAFYAGHRDEALQLFQQIKDSHLTTAKYLLVQNYLAAEAQLKDFFTEVEWLL
                     HDKPVREFDYYYDQVVCVGELLSTSIMSAFLAESGIGNQWIDVRDIFRTDANFRDAAI
                     DWEVTGRKVNEEVLPLFAEDDLVVTQGFIGATSDNESTTLGREGSDYTAAVFANLLDA
                     ESQTIWKDVEGVMNADPKQFPDAVMIHELNYDEVIEMAYYGAQVIHPKTIKPLQNKDI
                     PLYVKCFLDASLPGTVIHNKSLKGLPPIIVLKQDQVLVQLHSKDFSFVGETGMARLYD
                     LFGQVRVKPNLIQSGAVSIQVCLDDRPSRIERLALAAADLFDVQVERGLTLLTIRHYT
                     GEVLQRLTAGRQEVLRQQTKETVQVLMR"
     gene            complement(1295..>2107)
                     /locus_tag="VKQ52_15390"
     CDS             complement(1295..>2107)
                     /locus_tag="VKQ52_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88638.1"
                     /translation="SLGLNYPNASDRILSDSLQPGKDIYIHGSCVTTGCIPITDNQIE
                     ELYILAAHAKDLGEDFIPVHIFPVNFNNARSVEYLNKYLQTFSEYTPFAKSMRSAFYY
                     FEKYREVPFVMVNGKGEYVTEEVAPAEAEVKIPNVEPVTPATAAAKKKPVPTRPVRVN
                     PIPEEQLAKTVDHLPVYPGGNEAFQDFLNKLGKDEVKDLDEGQSKAFIMVEYVIDAVG
                     KPVFAKVIRGGNDGLNTKVEDAFLGMPQWSPAAYSGVRVPIRLKQTVMIGIN"
BASE COUNT          452 a          513 c          672 g          470 t
ORIGIN      
        1 caatgaaaat tttcaaattc ggcggggcga gtgtcaacag cgtggatcgc atccggcaga
       61 taccgggcat actgcggcaa tattccgggc aaaagatatt ggtggtcatt tccgcgatgg
      121 gcaagacgac gaacgcgctg gagaaagtgg ccgaggcctt ttatgccggt caccgcgacg
      181 aggccctgca gcttttccag cagataaagg atagccatct gacgacagcg aagtatctcc
      241 tggtgcagaa ttacctggcg gcggaggcgc agctaaagga tttttttacg gaggtggaat
      301 ggctgttgca cgataagccc gtccgagaat tcgattacta ctatgaccag gtggtttgtg
      361 tcggagagtt gttgtccact tcgatcatga gtgccttcct tgcagaatcg ggcatcggaa
      421 atcaatggat cgacgtcagg gatatcttcc gcacggatgc gaatttccgc gatgcggcca
      481 tcgactggga ggtgacgggc cggaaggtga acgaggaggt attaccgctt ttcgcggagg
      541 atgaccttgt cgtgacccag ggattcatcg gagccacctc ggataacgag agcacgacgc
      601 tgggccggga agggagcgac tatacggcgg ccgtctttgc caatctgctc gacgcggaaa
      661 gccagaccat ctggaaagat gtcgaaggtg tgatgaatgc cgatccgaag cagtttcccg
      721 atgccgtgat gatacacgag ctcaactatg acgaggtcat cgaaatggcc tattatgggg
      781 cgcaggtgat ccatccgaag acgatcaagc ccttgcaaaa caaggacatc ccgctttacg
      841 tgaaatgttt tctcgacgcc tccctgcccg ggacggtcat tcataataag tcgctgaagg
      901 gactaccgcc gatcatcgtg ctgaagcagg accaggtgct cgtgcagctg cattcaaagg
      961 atttcagttt tgtcggcgag acaggaatgg cgcggttgta cgatcttttt ggccaggtgc
     1021 gggtgaagcc caacctgatc cagagcgggg ccgtgagtat ccaggtgtgt cttgacgacc
     1081 ggccttcgag gatcgagcgg ctggcgctcg cagcagccga ccttttcgat gtgcaggtgg
     1141 agcgggggct gaccctgctg acgatccggc actataccgg agaggtgctt cagcgactga
     1201 ccgcggggcg acaggaggtg ctgcggcagc agacgaaaga gaccgtacag gttttgatgc
     1261 gataggcggt tgagcgccgg gatcggtcgg gggatcagtt tatcccgatc atgaccgtct
     1321 gttttaggcg gatggggacg cggacgccgg aataagcggc gggagaccat tggggcatgc
     1381 cgaggaaggc gtcttcgact ttggtattga ggccgtcgtt acccccgcgg atgaccttgg
     1441 cgaagacggg tttgccgaca gcgtcgatga catattccac catgataaag gcctttgact
     1501 gaccttcatc gaggtctttt acttcatctt tacccaattt attgaggaaa tcctggaagg
     1561 cttcgttgcc gccggggtag acgggcaggt ggtctaccgt tttggccagt tgctcctccg
     1621 ggatagggtt gacgcggaca ggccgggtgg gcacgggctt ctttttagcg gccgcggtgg
     1681 caggagtcac gggttcgaca ttggggatct ttacctcggc ttcggcgggc gccacctctt
     1741 cggtaacata ttcccctttc ccgttgacca tgacgaaggg cacttcgcgg tacttctcga
     1801 agtagtagaa ggcactgcgc atgcttttgg caaagggggt gtattcggag aaggtctgta
     1861 agtacttgtt gaggtattca acgctgcggg cattgttgaa attgacgggg aagatatgca
     1921 ccgggatgaa gtcctcaccc aggtctttcg cgtgggcggc gagaatatag agttcctcga
     1981 tctggttgtc cgttatagga atacaccccg tcgtcacgca gctgccgtgg atatagatgt
     2041 ccttcccggg ctgcagggaa tcactcagaa tgcgatcgga agcgtttggg taattcaggc
     2101 ccagcga
//
LOCUS       DATGFG010000817         7226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_347720, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000817 DATGFG010000000
VERSION     DATGFG010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347720"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..920
                     /locus_tag="VKQ52_15395"
     CDS             42..920
                     /locus_tag="VKQ52_15395"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLZ88639.1"
                     /translation="MALNLSILKTRSLTAAVFVVVMLAGLLWNPWSFLLLFTFIHFGC
                     WVEYQKIMVLIDKDYAGISTLHRYSVMIAGWCLMLYLVNGNLRLAGLSLHVVGWWGCF
                     GFLCLLVAGEIIQYRTLNIKNIGRSGLGLIYISLPLALLTWLRTKFYGSPFPFTDRLP
                     VLMIIGAIWINDTMAYFVGSLIGRTPLSAISPKKTVEGTIGGIILAIGAMGGIGALLT
                     SDWNPFSSIHWMIVAAICAIMGTLGDLLESKLKRMAGIKDSGRIMPGHGGFLDRFDSL
                     LVATPFAWCYLVWVIG"
     gene            1000..1659
                     /locus_tag="VKQ52_15400"
     CDS             1000..1659
                     /locus_tag="VKQ52_15400"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HLZ88640.1"
                     /translation="MTIHREGYKSIAIGTLAFAVINLISFYFISAHAAWLSGLLFVVT
                     LGLLLFLISFFRVPARQLSTGDNLIICPADGKVVVIEEVVDTEYFKGKRLQLSIFMSP
                     ANVHQNRNPVGGEVVYNQYHQGKYLVAWHPKSSTENERHSVVIRHAQGELLVKQIAGA
                     LARRIVNYLHVGQKVEQSAEMGFIKFGSRVDVLLPPGTPVEVQLNQVVQGGVTVLAKW
                     K"
     gene            1698..1877
                     /locus_tag="VKQ52_15405"
     CDS             1698..1877
                     /locus_tag="VKQ52_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88641.1"
                     /translation="MKKVFLLQMAVLLFTGVSFAQTGKDADKAKAKDTTAVKAAKKPM
                     PKACPGKTCGKKKSD"
     gene            complement(2035..2736)
                     /locus_tag="VKQ52_15410"
     CDS             complement(2035..2736)
                     /locus_tag="VKQ52_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HLZ88642.1"
                     /translation="MQYKYKHLFFDLDHTLWDFEANSRLTLHELYHTLDLAARGVSDF
                     DLFHKYYILHNDKLWERYRNGYIKVDELRWKRMWLTLLDFKIGDEPLARRLGEQFLDA
                     LPTRKLLFPYTTEILTYLRGRGYQLHLITNGFEKTQHSKLEYSGLSGYFGEVITSEGS
                     NSLKPHKEIFEYAFRKTGADPAASIMLGDSIEVDIQGAINAGIDQVYVNHLGVEPAIK
                     PTYTVSSLKELENIF"
     gene            2754..3455
                     /locus_tag="VKQ52_15415"
     CDS             2754..3455
                     /locus_tag="VKQ52_15415"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ88643.1"
                     /translation="MNCIITGASKGLGKAMAERFAAGGYDLYLCSRDAGRLAATKEEL
                     SGRFPAIGINCRPVDMGEKEQVMAFADWIAEAGATVDVLINNAGQFIPGSVHDEPEGV
                     LEKLMAVNLYSAYHLTRRLLPAMIARRSGHIFNICSVASLQAYPNGGAYGISKYALAG
                     FSANLREEMKPHGIKVTTVYPGAAFTDSWAGSGVDPERIMTAGDVAEMVFTASRLSPQ
                     ATVEEILLRPQLGDL"
     gene            3685..5679
                     /locus_tag="VKQ52_15420"
     CDS             3685..5679
                     /locus_tag="VKQ52_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HLZ88644.1"
                     /translation="MKFTTVVSSQDVVKSDYLQVEFVVENAKQIDEMTPPDFPGFRIV
                     QGPIQSSGMSIVNGNMSQYKGLSFVLEPAKTGKFTIGGAVATVDGKQMRSNSVVVTVH
                     PGGSGSANSRSTRPGGGGPVGNPFLQPSWPDPFGREPNEVERDYVLKPGENVKEKIRK
                     NLFVRVQVDKNTCYVGEPIVATYKLYSRLSSESRVTKRPSLNGFSVYDMVDPSSDPVS
                     VEKLNGKDYTVHIIRKTQLIPLQSGSIDLDPVEVENTVHFVKGGHQESRRSGNSLRDL
                     LDQMNDESNLGPEIDENVTLDTKPVTITVKPLPEENKPVGYGGAVGNFSVEASLGSNT
                     VAAQDEATLHLVVKGKGNLPVIAAPPVAWPDSIRAFDPTAKEDVNKTVVPMTGTKAFD
                     YVFTPRSAGHYTIPAVNFSFFDPATQTYKTAGTKPLDLQVSPAVRRQHSPASTDVARA
                     DEPVSGRIGNFLRDRLEWVFAALLVAGVTIYFWRQNSRLRKISGERKPTVPAVTAVTQ
                     PVTTDPSNAPQQPVAAQHPIAGQPGMTSNAPQYPGAGERQPIPAMAAASYVPAVPATD
                     PLLDARQFCDNGDSKAFYREINRAIWKAMVRKLELPASELNKQNSIRQLRLRGWDETS
                     LLSLENVLNECEMNLYTPAYDRFNMELLLRQTERLIDRLG"
     gene            complement(5919..6620)
                     /locus_tag="VKQ52_15425"
     CDS             complement(5919..6620)
                     /locus_tag="VKQ52_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88645.1"
                     /translation="MSSKISILLVEDEENLHEALKLNLELEGYEITSAFDGQQALKAV
                     QSEYFDLIILDVMLPGVDGIGVTETIRVQNNDVPILILSAKNASADRVLGLKKGADDY
                     LTKPFNLEELLLRIQKLIEKNKKMQEKESSGDVYSFGKNIIDFKAQEATTKDGERIQL
                     SKKEAMLLKLLIENRNEVVTREKILQAVWGYNVYPTTRTIDNFVLNFRKYFEEDSRNP
                     KYFHSVRGVGYKYTE"
     gene            complement(6708..>7226)
                     /locus_tag="VKQ52_15430"
     CDS             complement(6708..>7226)
                     /locus_tag="VKQ52_15430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ88646.1"
                     /translation="QEANRLDNLATNILVASQLEGEYVQAKESIDLSDLVHRSVQEYL
                     HRFPERSWAEQIEPGVTIIGDLLLLQLLVSNLVENAIKYSPREGLITIRLERRGRSIW
                     LSVADQGEGIPDEEKKKIFHKFYRTGQEATRKTKGTGLGLYLCRKIAEDHNAILKVAD
                     NSPVGSIFTVVF"
BASE COUNT         1490 a         1828 c         2236 g         1672 t
ORIGIN      
        1 tcctccgatg tcagttcgcc ctccgatgtc aatatttatt aatggcactg aacctctcaa
       61 tactaaagac ccggtcgtta acggctgccg tattcgtcgt cgtcatgctg gcggggctgt
      121 tatggaaccc ctggagtttt cttctcctgt tcacctttat tcatttcggc tgctgggtcg
      181 agtaccagaa gatcatggtc cttatcgata aggactatgc ggggatctcc acgctccacc
      241 ggtacagcgt catgatcgcc ggctggtgtc tgatgctgta tcttgtcaac ggcaacctgc
      301 ggctggcggg gctttcgtta cacgttgtgg gctggtgggg ttgttttggt tttctttgcc
      361 tgttggtggc gggggagatc atccagtacc ggacgctgaa tataaagaac attggccgga
      421 gcgggcttgg gctgatctat atctcgctcc cgctggccct gctgacctgg ttgcggacga
      481 aattctatgg cagcccgttc ccttttaccg atcggctccc cgtcctgatg atcatcggcg
      541 ccatctggat caatgatacg atggcctatt tcgtcggttc gctgatcggc aggacgccgc
      601 tgtcggcgat ctccccgaag aagaccgtgg aagggaccat cggcgggatc atcctcgcca
      661 tcggggcgat gggcgggatc ggtgcgttgc tgacctcgga ctggaatcct ttttcctcca
      721 tccactggat gatcgttgcc gctatctgcg ccatcatggg aacccttggt gatctgctgg
      781 agagtaagct gaaacgcatg gccggtatca aggacagcgg gcgtatcatg cctggtcatg
      841 gcggattcct tgaccggttc gactcgttgc tggtggccac gccatttgcc tggtgttatc
      901 tcgtatgggt gatcgggtga tggttgagcc gggaaccgac aaaatgcgtg tcccgaaacc
      961 accaaaatgc gtattttgca cgttcaactt gttgtactaa tgacaattca tcgggaaggg
     1021 tataagtcca ttgctatcgg caccctggct tttgccgtca tcaatcttat ctctttttat
     1081 tttatcagtg cccatgcggc ctggttgagc gggctccttt tcgtggtaac ccttggactt
     1141 ctcctgttcc tgatctcctt tttccgcgtt cccgcgcgtc agctctccac cggggacaac
     1201 ctgatcatct gtcccgcgga cgggaaggtg gtggtcatcg aggaggtggt ggatacagaa
     1261 tattttaaag gcaaacgact ccagctctcc atttttatga gcccggcgaa cgtccaccag
     1321 aaccggaacc cggtgggtgg cgaggtcgtc tacaatcaat atcaccaggg gaaatacctg
     1381 gtggcctggc atcccaaatc ctccacggag aacgagcggc atagtgtcgt tatccggcat
     1441 gcgcagggag agctgctggt gaaacagatt gccggcgccc tggcgcggcg gatcgtcaac
     1501 tacctgcatg tagggcagaa ggtggagcaa agcgcggaga tgggctttat taaattcggc
     1561 tcgcgggtcg atgtcctgct gccgccggga acgcccgtcg aagtgcagtt gaaccaggtc
     1621 gtccaggggg gtgtgacggt cctggcgaag tggaaatgat tgtttaaaaa gtgaaaatga
     1681 ttgtttaaat tgctgatatg aagaaagtgt tcctgctcca gatggccgtc ctgcttttta
     1741 ccggtgtctc ctttgcacag accgggaagg atgccgacaa ggcaaaggcg aaggatacga
     1801 cggcggtgaa ggcggcaaag aagccgatgc caaaggcctg cccgggcaag acctgcggca
     1861 agaagaaaag cgattagcaa ggggaagagc gaagaaggag agcgactggc aataagcaat
     1921 aagaaggacg gccagcaaga agatgcgcga agaagaagcg cgattagcaa gaggaagcgc
     1981 gcaaaagaag agcgaccagc aagaagaggc gcaaagaaga agagcgaaga gcgatcagaa
     2041 gatattctcc aattctttta gggaagacac ggtatacgtg ggttttatcg cgggttcgac
     2101 ccccaggtgg ttgacatata cctggtcgat cccggcattg atcgcgccct ggatatccac
     2161 ttcaatgctg tcgccgagca tgatgcttgc ggccggatcg gcgcccgtct tccggaaggc
     2221 atattcaaag atctccttat gcggtttcag gctgttggag ccttcggagg tgatcacctc
     2281 cccgaaataa ccgctcaatc ccgaatactc caatttgctg tgctgggtct tctcgaatcc
     2341 gttggtgata agatgcaact ggtatcccct tcccctgagg taggtgagga tctcggtcgt
     2401 ataggggaag aggagcttgc gggtgggcag ggcatcgagg aattgttcgc ccaatcggcg
     2461 ggcgaggggt tcgtcgccga tcttgaaatc gagcagggta agccacattc ttttccatcg
     2521 cagctcatcc accttgatat agccgttgcg atagcgttcc cacagtttgt cgttatgaag
     2581 gatataatat ttgtggaaca ggtcgaagtc ggacactccc cgggcggcga ggtccagggt
     2641 atggtatagt tcatgcaggg tcagccggct attcgcctca aaatcccaaa gggtatgatc
     2701 caggtcgaag aaaaggtgtt tatacttata ttgcatggcg caatttagta attatgaact
     2761 gtatcatcac cggagcttca aagggactgg ggaaggccat ggcggaaagg tttgccgcgg
     2821 gcggttatga tctgtatctc tgttcacggg atgcgggcag gcttgccgcg acaaaggaag
     2881 agctctccgg tcgctttccg gcgatcggga taaactgccg gccggtggat atgggcgaaa
     2941 aggaacaggt catggccttt gcggattgga tcgcggaggc gggtgcgacg gtggatgtgt
     3001 tgatcaacaa tgccgggcag ttcatcccgg gcagcgtgca cgacgaaccg gagggggtac
     3061 tcgaaaaatt gatggccgtc aatctgtaca gcgcctatca tcttacccgg cggctcttac
     3121 cggcgatgat cgcgcgccgg agcggtcata tttttaacat ttgttcggtg gcctccctgc
     3181 aggcctatcc caacgggggc gcctacggca tcagcaaata tgcgcttgcc gggttctcgg
     3241 ccaatctgcg ggaagagatg aaaccccatg ggatcaaggt gacgacggtg tatcccgggg
     3301 ccgcttttac cgattcctgg gcgggctcgg gcgtagatcc tgagcgcatt atgaccgcgg
     3361 gagatgtggc ggaaatggtc tttacggcca gccggctttc gccccaggca acggtggagg
     3421 agatcctgct gcgcccgcag ctcggtgatc tgtgagggcg attgctgaga ggggtgtttg
     3481 ggggcgatgg tcaggggcgt cctattccgg gaagcgcatg ccgggaccca ttctttaact
     3541 gagttttaac ccttatgaat cttatatttg taagcggcta tggaatttgg tagaccaata
     3601 aaatcacccg ttatcgcgac tcttctacgc ttatcgttta ttactctgat agcattcgcc
     3661 ccggggcttt acgccgtggg gcaggtgaag ttcaccactg ttgtcagcag tcaggacgtc
     3721 gtgaagtccg attatctcca ggtggaattt gtcgttgaga atgccaaaca gatcgatgaa
     3781 atgacgcccc ccgacttccc tggttttcgg atcgtccagg gccccattca atccagcggc
     3841 atgagcatcg tcaacgggaa tatgtcccaa tacaaggggc tgtcctttgt gctggaaccg
     3901 gcgaagacgg ggaagttcac gattggcggc gctgtggcga ccgtcgatgg caagcagatg
     3961 cgcagcaatt ccgttgttgt tacggtgcac cccggcggat cgggttccgc caatagccgg
     4021 tctacgcggc cgggtggcgg cggtcctgtg ggcaatccct ttttacaacc ttcctggccg
     4081 gacccttttg gccgcgagcc caatgaggtg gaaagggatt atgtgttgaa gccgggagag
     4141 aatgtaaaag aaaagatccg caagaacctt tttgtaaggg tgcaggtgga taagaacacc
     4201 tgttatgtag gtgagccgat cgtggccacg tataaattat actcccggct cagcagcgag
     4261 tcccgggtga cgaagcggcc ttcgctcaac ggcttcagcg tttatgacat ggtggacccc
     4321 agctccgatc ccgtttcggt ggagaagctc aacggaaagg actataccgt tcatatcatc
     4381 cgcaagaccc agctcatccc gctgcagtcg gggtcgatcg acctggaccc cgtggaggtg
     4441 gagaataccg tgcattttgt aaagggcgga caccaggagt cgcggcgcag cggcaacagc
     4501 ctgcgcgatc tcctggacca gatgaacgat gaaagcaacc tgggccctga gatagacgaa
     4561 aatgtcaccc tcgatacgaa acccgtgacg atcaccgtaa agccgctgcc ggaagagaac
     4621 aagccggtgg gctatggcgg cgccgtgggt aatttttcgg tggaggccag tctgggaagc
     4681 aataccgttg ccgcgcagga cgaggccacc ctgcatcttg tcgtaaaagg aaaggggaat
     4741 ctcccggtca ttgccgcccc gcccgtcgcc tggcccgata gcatccgggc ttttgacccg
     4801 acggcaaagg aggatgtgaa caagacggta gtaccgatga ccggaacaaa ggcgttcgac
     4861 tatgtcttta cgccgagatc ggcaggacat tataccatcc cggccgtaaa cttctccttt
     4921 ttcgatcccg ccacacagac atataaaaca gcggggacga aacccctgga tcttcaggta
     4981 tcgcccgccg ttagaaggca acatagtccg gcctctaccg acgtcgcccg ggccgatgaa
     5041 ccggtatcgg ggcgtatagg caacttcctc cgggaccgcc tggaatgggt gttcgcggcc
     5101 ctgcttgtcg ccggggttac gatctatttc tggcggcaga acagcaggct ccggaagatc
     5161 agcggggagc ggaagccaac ggttccggcg gtaacggcgg tcacgcaacc tgtgaccacg
     5221 gatccttcca atgcaccgca gcaacccgtt gccgcgcagc atccgatcgc ggggcaaccg
     5281 ggcatgacgt cgaatgcgcc gcaatacccg ggcgccggcg aacgacagcc catcccggcg
     5341 atggccgccg cctcgtatgt tccggcggtt cccgccaccg atccgctgct ggatgcccgg
     5401 caattttgcg ataacggcga ttccaaggcg ttctaccggg agatcaaccg ggccatctgg
     5461 aaggcgatgg tgaggaagct ggagctaccc gccagtgagc tgaacaaaca gaacagcatc
     5521 cggcagctgc ggttgcgggg ctgggatgag acctccctgc tttcgctgga aaatgtcctg
     5581 aatgagtgcg agatgaatct ttataccccg gcgtatgacc gcttcaatat ggagttgctg
     5641 ctgcggcaga cggagcgatt gatcgacagg ctgggttgag aggtggggct ggcaggtggg
     5701 gttagggagg ttcgggagtt ggagccgatg tgggttaggg aggcccgaag gttcacggtc
     5761 ggtgtgggtt acggaggccg gggggcgagg ccgatgtgga ttgcagaggt ccgggggttg
     5821 gagccgatgt gggttaggga ggttcgggct tcaggtaagg gccgatgtgg gggtgtgggt
     5881 tacggagggc tgggcttcgc ggccgatggt gttgcctgtt attcggtgta tttatacccc
     5941 accccgcgga cagaatgaaa atacttgggg ttgcggctgt cctcttcgaa atacttcctg
     6001 aaattcagaa caaaattatc gatggtgcgg gtggttggat agacgttata gccccagacg
     6061 gcctgcagga tcttttcccg tgttacgact tcgttcctgt tctcgatgag taatttcagc
     6121 agcatcgctt ctttcttgct gagctggatg cgctccccat cctttgtagt ggcttcctgg
     6181 gctttgaagt cgatgatatt ctttccgaag ctgtatacgt cgcctgagga ctctttctcc
     6241 tgcatcttct tattcttctc gatcagcttt tggatccgga ggagcagttc ttcgaggttg
     6301 aagggttttg tgaggtagtc gtcggcgcct tttttcaggc ccagtacccg gtcggcgctg
     6361 gcgttctttg cgctgaggat gaggatgggc acatcgttgt tctggacgcg gatggtctcg
     6421 gtgacgccga tgccgtccac gccgggcagc attacgtcga ggatgatcag gtcgaagtat
     6481 tcggactgga cggctttcag ggcctgctga ccgtcgaagg cggaggtgat ctcataccct
     6541 tccagctcga ggttcagctt gagcgcttcg tggagatttt cttcgtcctc taccagcagg
     6601 atggatattt tagagctcat gttgtattag catttgccgt aagggggcgc ctttggaatt
     6661 gcccgaaagg gttttggtaa aaataaccct ttaggggcct ggaatactta gaatacgacg
     6721 gtaaaaatac tcccaaccgg cgaattatcg gcgactttca ggatggcatt atggtcttcg
     6781 gctatcttcc ggcagagata gagtccgagg ccggtgcctt ttgtcttgcg ggtcgcttcc
     6841 tggccggtgc ggtagaattt atggaagatc ttcttttttt cttcgtcggg gatgccctcg
     6901 ccctggtcgg cgacggatag ccaaatggat cgtccccgcc gttcgaggcg gatggtgatg
     6961 aggccttcgc ggggggaata cttgatggcg ttctccacga ggttgctgac cagcagttgc
     7021 agcagcagca gatcgccgat gatcgtcacg ccgggttcga tctgttccgc ccaggaccgt
     7081 tcgggaaacc ggtggaggta ttcctgtacc gaccggtgga cgagatcgct gaggtcgatg
     7141 gattcttttg cctggacata ttctccttcg agctgggagg cgacgaggat attggtggcg
     7201 aggttgtcga ggcggttggc ctcctg
//
LOCUS       DATGFG010000818         4621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_336712, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000818 DATGFG010000000
VERSION     DATGFG010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336712"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VKQ52_15435"
     CDS             <1..338
                     /locus_tag="VKQ52_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88647.1"
                     /translation="SKTEHLGADSSVQRTEDENTIIEFDKSTISITPGHEPTMTGTVN
                     SYTCNWATPFKVGRSRLKITLTNPQGETKNATINIEGKDGKIIFLAELDDEADKKIRL
                     VVDKFEEKH"
     gene            388..960
                     /locus_tag="VKQ52_15440"
     CDS             388..960
                     /locus_tag="VKQ52_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88648.1"
                     /translation="MQTNPPMAVRFFRRHKHIILYGLALALLLFLLKWLEWRFIVFDH
                     AIEVYSGAIAVLFTGLGIWLALKLARPKVATIVIEKEVPAAAGAAGVAPAAGAASAVP
                     AAPAVPAAADGIAVPIPDEKELAKLGLSQRELEVLQLIAEGLSNQEIAGRLYLSLNTV
                     KTHSSRIFEKLDVRRRTQAVDKAKRLRLIA"
     gene            1145..1690
                     /locus_tag="VKQ52_15445"
     CDS             1145..1690
                     /locus_tag="VKQ52_15445"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HLZ88649.1"
                     /translation="MKRNILIFGLIAGLILGLFTLSITILNGKGTDAPHSEIVGYTAM
                     VVAFSFIFVGTKNFRDKYNGGVVSFGKALRIGLGITLIGSTMYVIAWMIEYYLFIPDF
                     MDKYAAHMIHEIQASGVSPAAMNKQIAEINHMSQLYKNPVFVALFTYVEVLPVGIIIS
                     LLTSLLLKRKTPRPGKTQLAV"
     gene            complement(1706..2038)
                     /locus_tag="VKQ52_15450"
     CDS             complement(1706..2038)
                     /locus_tag="VKQ52_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88650.1"
                     /translation="MQVGDYLHDNIGIIGNDQVSILSDDQTRLISYSSTFFALSDRIA
                     FEGADVYFQKVQDATGQISDALKHIDKVDKVICISASVIGLAGGIVSGNTGMIISALQ
                     AIKGQINV"
     gene            complement(2105..2935)
                     /locus_tag="VKQ52_15455"
     CDS             complement(2105..2935)
                     /locus_tag="VKQ52_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88651.1"
                     /translation="MPLSLRNCGLLLSAAVVFTRCANLKEVSSFAITSQQIMDRNKSA
                     TFGYYAYFHDSAYIYRYLPDHLRDVDCHCDIEKQADAHIASEYSLLSTYFGVLAKFAD
                     PRAAINFGPMGNPLPAGAYGHVSISAQEATLTARLATGLSVLVTTRYKEKKARLFIAN
                     YRDSVAPLITLLKIRADNLSGRIVNLQLQLERVTDSLISFAEHKEVKMPVLFVYEQKR
                     KELDETLALYEQRTHELETMIAGGQLIVDNIDNLHSQPFKDKMKSVVNALVLNANVKF
                     "
     gene            3195..4214
                     /locus_tag="VKQ52_15460"
     CDS             3195..4214
                     /locus_tag="VKQ52_15460"
                     /inference="COORDINATES: protein motif:HMM:NF021553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2278 family protein"
                     /protein_id="HLZ88652.1"
                     /translation="MPIQNYGVVQGRVFDRVLATKKDEHYEILINRGDNPQRVAINTQ
                     SSTPPSQVLFYANLDFQHPITDAILQANLPAGYTPLESKPGGIALDFIRENLFPIDQL
                     VPLPATSTGTSNDLNDQLDLTVQQAITDTDAVLYAFGQHWTDTKADPIFPEINPSIGI
                     HDIHMNQGNPQGSFYKDNGVYQDGGLIFHFPSRNRWAAIFTAFQSESFHTDDQTGDPL
                     VAAGPGNPQNPENSSTVSIAAALVNPKGGDPGKEYVLLLNKGSEAVDLSGWKIADSHK
                     NKDTISKQAIQPGDTLRVNLSGHGAQLSNKGGIITLLNPQGLKVDGVTYTRRDVVKEG
                     VLVEF"
     gene            complement(4233..4427)
                     /locus_tag="VKQ52_15465"
     CDS             complement(4233..4427)
                     /locus_tag="VKQ52_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88653.1"
                     /translation="MSRHQDNKKNERNPARDKTGRDPHPQPRAVNPFEKKKETPPEKA
                     AEKEQADLEQQHKEADTERD"
BASE COUNT         1048 a         1399 c         1213 g          961 t
ORIGIN      
        1 cctcgaagac cgagcacctc ggcgccgaca gcagcgtcca acgcaccgaa gacgagaaca
       61 ccatcatcga attcgacaaa tcgaccatca gcatcacccc gggtcacgag cccacgatga
      121 ccggcaccgt caactcctac acctgcaact gggcgactcc cttcaaagtg ggacgcagtc
      181 ggttgaagat caccctcacc aacccccagg gcgagaccaa gaatgccacc atcaacatcg
      241 aaggcaagga cggcaagatc atcttcctcg ccgaactcga cgacgaggct gacaaaaaga
      301 tccggctggt cgtggacaaa ttcgaagaaa aacattaggc gaaacacccc gcttcccgat
      361 cccgccaaac aatagtattt ttggggtttg caaaccaacc cacccatggc ggtccgcttc
      421 tttcgtcggc ataaacatat catcctctac ggattggccc ttgccctgct gctcttcctt
      481 ttgaaatggc tggaatggag attcatcgtc ttcgaccacg ccatcgaggt ctacagcggg
      541 gcgatcgccg tcctcttcac cggattgggt atctggctcg ccctaaagct ggcccggcca
      601 aaggtggcga cgatcgtcat cgaaaaggaa gtgcccgccg ccgccggagc cgctggtgtt
      661 gcccctgccg ccggagccgc ctcagccgtc cccgccgccc ctgccgttcc cgctgccgcc
      721 gacggaattg ccgtccccat ccccgacgaa aaggaactcg ccaaactggg cctcagccag
      781 cgggagctcg aagtcctgca attgatagcc gagggcctca gcaaccagga gatcgccggc
      841 cgcttatacc tttccctcaa cacggtcaag acgcattcct cccggatctt cgaaaagctc
      901 gacgtccgtc gccgcaccca ggccgtcgac aaggcaaaac ggctaagact catcgcctag
      961 ggcggggcat ggcgctgcgc cgatcacgca aaggggcggg cacggtccac gcaacaggcc
     1021 ggcacgtctc gctcagccgc ctcacaccaa agggtgaaaa cgcggccggc cgccaaaatc
     1081 acccaaaagt atgaaccgga tttttcgcgg atagcgcatt tttggtccac aaaacaatcg
     1141 caacatgaaa aggaacatcc tcatcttcgg actgatcgcc gggctcatcc tcggcctctt
     1201 caccctctcc atcaccatcc tgaacgggaa gggcaccgac gccccacaca gcgagatagt
     1261 ggggtacacc gccatggtcg tggccttctc ctttatcttt gtcggcacca aaaatttccg
     1321 tgacaagtac aacgggggcg tcgtcagctt tggcaaggcc ctgcgcatcg ggctgggcat
     1381 caccctcatc gggtcgacga tgtacgtcat cgcctggatg atcgagtact atctcttcat
     1441 ccctgatttc atggacaaat atgcggccca tatgatccac gagatccagg ccagcggcgt
     1501 cagccccgcc gccatgaaca aacaaattgc ggagatcaac cacatgagcc aactatacaa
     1561 gaacccggtg ttcgtcgcgc tgttcaccta cgtcgaggtc ctccccgtag gcatcatcat
     1621 ctccctgctc acttccctcc tgctcaagcg gaagaccccg cgcccgggca agacgcaact
     1681 ggcggtatag gccggccggg cgaatctaaa cattgatctg ccctttgatc gcctgcaacg
     1741 ccgagatgat catgcccgtg ttcccgctca cgatcccgcc ggccagcccg atcacactcg
     1801 cggaaatgca gatcaccttg tccaccttgt cgatatgctt cagcgcgtcg ctgatctgcc
     1861 ccgtggcgtc ctgtactttt tgaaaataga cgtccgctcc ctcgaaggcg atgcggtcgg
     1921 aaagcgcgaa gaaggtactg gaataggaga tcagccgggt ctgatcatcc gagagaatgg
     1981 acacctggtc attgccgatg atcccgatat tgtcgtgcag ataatccccc acctgcatgg
     2041 cgagggtctg gtagaagcgg ccgacaagat tggcaaactg cgccgcggcg acttaatcca
     2101 taggctaaaa ttttacgttg gcgtttaaga caagggcatt gaccacactc ttcatcttat
     2161 ccttgaaggg ttgggaatgg aggttgtcga tattgtcgac gatcagttgg cctccggcaa
     2221 tcatcgtttc cagctcatgc gtccgctgtt cataaagcgc cagcgtctcg tcgagctcct
     2281 ttcttttctg ttcatagacg aacaggaccg gcatctttac ttctttgtgt tcggcgaaac
     2341 tgataaggga atccgtgaca cgttccaatt gcaactgaag attgacgatc cgcccggaga
     2401 gattatcggc acggatcttc agcagggtga tcagcggcgc caccgagtca cggtagttgg
     2461 cgatgaacaa ccgcgccttt ttctctttgt agcgggtggt gacgagaacc gacaatcccg
     2521 tggccagtct tgcggtaagc gtcgcctctt gcgccgagat ggatacatgt ccataggctc
     2581 cggcaggcag cggattaccc atcggcccga aattgatcgc cgcccgcgga tcggcaaact
     2641 tcgcgagcac gccaaaatac gtgctcagga gactgtactc actcgcgata tgggcgtcgg
     2701 cctgcttctc gatgtcgcaa tggcaatcga catcccggag atggtcgggc aggtagcgat
     2761 aaatataggc cgagtcgtgg aaataggcat aatagccgaa ggtggcggac ttgttcctgt
     2821 ccatgatttg ctgactggtg atcgcgaaag aactcacctc tttcaggttc gcacagcggg
     2881 taaagacaac cgccgccgac aacagcaagc cgcaatttct tagtgataag ggcatgtagg
     2941 ttaattagga ggaggcaaaa tagcacctta cgaggactca aagtaccaca tttttcgcca
     3001 cacacagtaa aaatttccag catacgaaca gcaaccaggc actggttttg atgatcatct
     3061 tttccatttc gcggtttatc cctataacaa acggggagtt aacaatagga caacattgca
     3121 gggagaaaac cggggttttt actgtaggcg gggggccgga aaaaaactat ccttgcagaa
     3181 acaaatttta ttctatgccg atccaaaatt atggtgtcgt acagggcagg gttttcgaca
     3241 gggtcctcgc aacaaagaaa gatgaacact atgagatcct catcaaccgc ggcgataacc
     3301 cgcaacgggt ggcgatcaac acccagtcca gtacgccgcc gagccaggtg ctgttctatg
     3361 ccaacctgga ttttcagcat ccgatcacgg acgccatcct gcaggcgaac ctccccgctg
     3421 gttatacccc gctcgaatcc aaacccggcg gcatcgccct cgatttcatc cgggagaatt
     3481 tgtttcccat cgatcagttg gttccgctgc ccgccacctc gacgggcacc agcaatgacc
     3541 tgaacgatca gctcgatctc acggtgcagc aggcgatcac cgacacggac gcggtgttgt
     3601 atgcctttgg ccagcattgg acggatacca aggccgaccc catcttcccg gagatcaacc
     3661 ccagcatcgg gatccatgat attcatatga accagggtaa tccgcagggc agcttttata
     3721 aggataacgg cgtttaccag gacggggggc tgatctttca tttcccttcg cgcaatcgct
     3781 gggccgccat cttcaccgcc tttcaatccg agtcctttca tacggatgat cagacaggcg
     3841 atccgctcgt tgctgccggt cccggtaacc cacagaaccc cgagaacagc tcgacggtca
     3901 gcatcgccgc ggccctcgtc aacccgaagg gcggcgaccc cggcaaggaa tacgtgctcc
     3961 tgctcaacaa gggcagcgag gcggtggacc tctccggctg gaagatcgcc gattcacata
     4021 agaacaagga caccatcagc aaacaggcca tccagcccgg tgataccctg cgggtgaacc
     4081 tcagcgggca tggcgcgcaa ctgtccaata aaggtgggat cattaccctc ctcaaccccc
     4141 aggggctgaa agtggacggg gtcacctata cacggcggga tgtggtgaag gaaggggtgc
     4201 tggtggaatt ttagggcgcg ctccgtagct gcctagtcgc gttccgtgtc cgcctctttg
     4261 tgttgttgct cgagatcggc ctgctccttt tcggcggcct tctccggggg tgtctccttt
     4321 ttcttttcaa atggattcac cgcacgtggt tgtggatggg gatccctgcc tgtcttgtcg
     4381 cgggcgggat tgcgctcgtt tttcttattg tcttgatgtc tggacatatg cgtgtttcat
     4441 atgtccatga aaaaatcata cccccggtcc gcgccagtcg ctcattgcga tgccccaacc
     4501 gcggggccag gccgccggtt actcaggcag gctcgcggca cacgctgggg atcaatacat
     4561 ttaccctttc aaacggggcc ttggccttag ttttgaatgg tataacttcg gggttccttt
     4621 c
//
LOCUS       DATGFG010000819         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_299598, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000819 DATGFG010000000
VERSION     DATGFG010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299598"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VKQ52_15470"
     CDS             complement(<1..1050)
                     /locus_tag="VKQ52_15470"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HLZ88654.1"
                     /translation="MALSRIWAAFILIAIVVAAVRYVTVPADKEIFGQMVTGKAGDTV
                     RVGAPAVAGGNPAGMKAAGVEAAHGAGAAVAAEQGYRVQQADGLIETCKNAVNLSLGL
                     IGIMALFMGLVSIAEAAGGMRFLARVTGPFFSKLFPDVPRGHPAMGHMVMNFSANLLG
                     LDNAATPFALKAMESLQEINPNKDTASNSQIMFLCLHASGLQVIPVSMIALRAAKGAP
                     DPTDIFVPIVIATFITTVASMCIVAAWQRINVFKPAILLWVGGISVIITALVLFLRSL
                     SADHVQSFSRALSGGLILLIFFLIVGAAVYKKVDVFGSFVQGAKGGFETALRIIPYLV
                     GMLAAISLLRVSGTFD"
     gene            1128..2228
                     /gene="ychF"
                     /locus_tag="VKQ52_15475"
     CDS             1128..2228
                     /gene="ychF"
                     /locus_tag="VKQ52_15475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HLZ88655.1"
                     /translation="MALQAGIVGLPNVGKSTLFNAVSNSAKAQASNYRFCTIEPNTGL
                     VDVPDERIDKLAELVKPERTVPTQVGIVDIAGLVKGASKGEGLGNKFLANIREVDAIV
                     HVIRCFEDENILRDEGAINPVGDKEIIDTELQLKDLESVEKKFSRVEKMLKTGDPKIK
                     AEYEILKRCKDHLEQGRSIRELDLSREERTAIADLFLLTEKPVLYVANVDEASMHTGN
                     KFSEALKASVAGENAQVIVMNNSIEAQISEMEDPADRQLFMDEYKMTEPALNKLIRSV
                     YRLLNLATYFTAGVQEVRAWTIHEGWKAPQAASVIHTDFEKGFIKAEVIAYADFIKFG
                     SEAAARDNGKLRIEGKEYTVKDGDVMHFRFNV"
     gene            complement(2336..>3553)
                     /locus_tag="VKQ52_15480"
     CDS             complement(2336..>3553)
                     /locus_tag="VKQ52_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ88656.1"
                     /translation="RLQPGSAAFNQAFSTLKTQAIPYGGKFNDQTNLYVGDAMYNFGD
                     VERTVDVTVGASTREFVLNSHGTIFADTAGRININETGAFAQLQRAFVDDLLKLSVSG
                     RYDKNSNFKGRFTPRATALFKIAKDNYIRASFQTAYRFPTTQNQFINLQTGSARLIGG
                     LPQFISYYGLNDGTTYDTAQLNKYAATGTPPTGYQYKEFKPETVASWELGYKGVIADK
                     LLIDVYGFYAQYTNFIGLTVLVKDPFATGSYVQNGANTFGIYTNSTNKVKTVGAGLSL
                     EYKLPNGFVASGNYAYNNISGNDNSQQTDFNTPKNKFNLSFANYTIAKYYGFNVTYRW
                     QQEYVYQSSFITGVTPAFGTLDAQISMKIPHMTSSVIKIGATNLLNKYYMDAIGNANI
                     GGLYYISFGYNIL"
BASE COUNT          874 a         1170 c          828 g          681 t
ORIGIN      
        1 atcgaaggtc ccgctcacgc gcagtaaact tatcgctgcc agcatcccca cgaggtaagg
       61 aatgatcctc agcgccgtct cgaaaccgcc cttcgccccc tggacaaacg acccaaaaac
      121 atccactttc ttatacacgg cagccccgac aatcagaaaa aagatcagca ggatcagccc
      181 cccgctcagc gcccgcgaaa agctttgcac atggtccgcc gaaagactgc gcagaaaaag
      241 caccagcgcc gttatgatga ccgagatgcc gcctacccag agaaggatcg ccggcttgaa
      301 tacattgatc cgctgccagg ccgccacgat acacatcgaa gccaccgtcg taataaaggt
      361 ggcgataaca atcggcacaa agatatccgt gggatccggc gccccttttg ccgcccgcag
      421 cgcgatcata ctcaccggta tcacctgcaa tcccgaggca tgcagacaaa ggaacatgat
      481 ctggctgttg gaagcggtgt ccttgtttgg gtttatctcc tgtagactct ccatcgcctt
      541 tagcgcaaac ggcgtcgccg cattgtccag ccccagcagg ttggccgaga agttcatcac
      601 catatgcccc atcgccggat gccccctggg cacatccgga aataatttcg aaaagaaagg
      661 ccccgtcacc cgggccagga agcgcatccc gcccgccgcc tccgcgatac tcaccagccc
      721 catgaacaag gccatgatcc cgataagccc cagggaaaga ttcacggcgt tcttacaggt
      781 ctcgataagg ccgtcagcct gctggaccct atagccctgt tcagcggcaa ccgcagcccc
      841 agccccatgc gcagcctcca cgcccgccgc cttcatcccg gccggattcc cacccgccac
      901 cgccggcgcc cccacccgca ccgtatcccc cgccttcccc gtcaccatct gcccgaatat
      961 ttctttgtcc gccggcacag tcacataccg caccgccgcc accacgatgg caatcaggat
     1021 aaaagccgcc cagatccgac ttaacgccat aataatctat tgtttacgtc ttaacgtact
     1081 caaattacca ttaattgcct aatttcgcgc ccacaattcg aaaattcatg gctttacaag
     1141 caggcatagt aggactacca aacgtcggga aatcaacctt gttcaacgcg gtcagcaaca
     1201 gcgcaaaagc acaggccagc aactaccgct tctgtaccat cgaacccaac acgggcctcg
     1261 tcgacgttcc ggacgaaagg atcgataaat tggccgagct cgtaaaaccc gaacgcacgg
     1321 tccccaccca ggtcggtatc gtcgatatcg ccggcctcgt aaaaggcgcc agcaaaggcg
     1381 aaggcctcgg caataagttc ctcgccaaca tccgcgaggt ggatgccatc gtccacgtca
     1441 tccgctgctt cgaagacgag aacatccttc gcgacgaagg cgccatcaac cccgtcggcg
     1501 acaaggagat catcgacacc gaactgcaat tgaaagacct cgaaagcgtc gaaaaaaagt
     1561 tcagccgcgt cgaaaaaatg ctgaaaacag gcgacccgaa aataaaggcc gaatacgaga
     1621 tcctgaagcg ctgcaaggac catctcgaac agggcagatc cattcgcgaa ctcgatctca
     1681 gcagggaaga acgcacggcc atcgccgacc tcttcctcct cacggagaaa cccgtcctct
     1741 acgtcgccaa cgtcgacgaa gcctcgatgc acaccggcaa taaattctcc gaagccctga
     1801 aagcctccgt cgccggcgag aacgcccagg tcatcgtcat gaacaactcc atcgaagccc
     1861 agatctccga aatggaagac cctgccgatc gccaattgtt catggacgaa tacaagatga
     1921 ccgaacccgc cctcaacaaa ctcatccgca gcgtataccg cctgctcaac ctcgccacct
     1981 acttcaccgc cggcgtacag gaagtccgcg cctggaccat ccacgaaggc tggaaagccc
     2041 cacaggccgc cagcgtcatc cacaccgact tcgaaaaagg cttcataaag gccgaggtca
     2101 tcgcctacgc ggacttcatc aaatttggtt cggaagccgc cgcccgcgac aacggaaaac
     2161 tccgcatcga agggaaagaa tacaccgtca aggacggcga cgtcatgcac ttccgtttca
     2221 acgtctgatc caccggtcct aagccggggc ccgcgcttcg ggcgggcccc ggccccccaa
     2281 aacgggaaag ccggtccctg cggaccggcc tccaatttcc tgcgctcgcg cgccattaca
     2341 atatgttata tccaaaactg atatagtaca atcccccgat attcgcattc ccgatcgcat
     2401 ccatataata cttattcaac aaattcgtcg ccccgatctt gatcacactg ctcgtcatat
     2461 gcgggatctt catactgatc tgcgcatcga gcgtcccgaa cgccggcgtg acccccgtaa
     2521 tgaacgaaga ctgatacaca tactcctgct gccagcgata cgtcacattg aacccataat
     2581 atttcgcgat cgtataattc gcaaagctca ggttgaactt attcttcggc gtattgaaat
     2641 ccgtctgctg gctgttatca ttcccggaaa tattattata cgcatagttg ccggaggcga
     2701 caaacccatt cggcagcttg tactctaaac taagccccgc acccaccgtt tttaccttgt
     2761 tggtgctgtt cgtatagatc ccgaaggtgt tggccccatt ctgcacatag gaccccgtcg
     2821 caaacggatc cttcaccagg accgtaagcc cgatgaaatt cgtatactgc gcatagaatc
     2881 catacacatc gatcagcagc ttgtccgcga tcaccccctt atacccgagt tcccaggatg
     2941 ccaccgtctc aggcttgaac tccttatact ggtagcccgt tggcggcgta cccgtcgccg
     3001 catacttgtt cagctgcgcc gtatcatagg tggtcccgtc gtttagtcca taatagctga
     3061 taaactgtgg caacccgccg atcagccgcg ccgaccccgt ctgcaggttg atgaattggt
     3121 tctgtgtcgt ggggaaccgg taggccgtct ggaatgaggc ccggatataa ttgtccttgg
     3181 ctatcttaaa cagtgccgta gccctcggag tgaaccggcc cttgaaattg gagttcttgt
     3241 cgtatcggcc cgataccgac agcttcagga gatcatccac aaaagcccgc tgcagctgcg
     3301 caaaggcccc cgtctcgttg atattgatcc ggcccgccgt atcggcaaag atggtcccgt
     3361 gggaattgag cacgaactcg cgcgtggagg cgcccaccgt cacatccacc gtcctctcca
     3421 catccccgaa attatacatg gcgtccccca cataaagatt ggtctggtca ttgaacttgc
     3481 ccccatacgg gatggcctgg gtcttgagcg tgctgaaggc ctggttaaag gccgccgagc
     3541 ccggctgtaa ccg
//
LOCUS       DATGFG010000820         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_697007, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000820 DATGFG010000000
VERSION     DATGFG010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697007"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VKQ52_15485"
     CDS             <1..682
                     /locus_tag="VKQ52_15485"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HLZ88657.1"
                     /translation="LLRKYTEQRDYPGIQGTSHLGLHLRFGTISIRALAREAAGTSDT
                     FLNELIWRDFYQMILWHFPWVGEGRAFKPAYDRIEWRNNEAEFEKWCAGQTGYPIVDA
                     GMRELNATGFMHNRVRMIVASFLAKHLLIDWRWGEAYFAGKLLDFDLAANNGGWQWAA
                     GSGCDAAPYFRVFNPTLQAKRFDPDHRYIRHWVPEWEELTYPRPIVEHEMARKRALEV
                     YSRAVKEK"
     gene            complement(669..1487)
                     /locus_tag="VKQ52_15490"
     CDS             complement(669..1487)
                     /locus_tag="VKQ52_15490"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HLZ88658.1"
                     /translation="MKFSLGFSPCPNDTFIFDALVNKKIDTEGIDFELFLEDVETLNQ
                     WAMQGKLDISKISYGVLPLVLPRYDLLDAGGALGKGVGPLLIARQPVSSTDINSCRIA
                     IPGQRTTAHLLFSLAFPDARKKEFMVFSGIEDAVLSGAVDCGVIIHENRFTYQQKGLV
                     KLLDLGEYWERETGAPIPLGGIVFRKGADATLSKKINSLIRKSLEYAFDRYPELPEFV
                     RSHAQEMDERVMRQHIDLYVNHYSLTLGQEGEKAIETLLQTYARIHQRVETTSL"
     gene            complement(1484..2152)
                     /gene="mqnB"
                     /locus_tag="VKQ52_15495"
     CDS             complement(1484..2152)
                     /gene="mqnB"
                     /locus_tag="VKQ52_15495"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HLZ88659.1"
                     /translation="MEILLVAATTFEIQPTIDYLRELTGKDRRHSVTPLITGVGGLAT
                     TWALTRQIDRGRPGLVLQAGIAGCFTGRKYGEVFVVREELLADLGVWEKGRFKTLFDM
                     RLAEPDHFPFAGGALVNPYGELLTLTGLEPVRGMTVNEITTDAKRIGYHQQNTAAVVE
                     SMEGGPMHYVCLQAGVPFLQLRSVSNAVGIRDKSKWDIPLAIARLNEELILLLEKLEK
                     INKA"
     gene            2224..2631
                     /locus_tag="VKQ52_15500"
     CDS             2224..2631
                     /locus_tag="VKQ52_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HLZ88660.1"
                     /translation="MVYLTRLEHFNAAHKLYNPGWTREKNESVFGKCANENWHGHNYE
                     LYVTIKGEPDPDTGFLFDVKTLGELIREHVIEKLDHKNLNLDVDFMRDKMCSTENLAI
                     GIWHQLLPHLPAGVRLHGIKLYETPRIYVEYFG"
     gene            2653..3075
                     /locus_tag="VKQ52_15505"
     CDS             2653..3075
                     /locus_tag="VKQ52_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HLZ88661.1"
                     /translation="MSGNERKVAVFRKEHFNAAHRIFNPAWDDRKNAEVFGKCSNPNY
                     HGHNYELIVKVTGVPDPETGYVMDMKVLSRITAEEVLEKFDHKNLNLDTVYFRDLNPT
                     AEHIAMVIYDLLRAKIEPRLDLQVRLYETERNFVEYPA"
BASE COUNT          740 a          816 c          838 g          699 t
ORIGIN      
        1 cctgctcagg aagtatacgg aacagcgcga ctaccccggt atccagggca ccagccacct
       61 cggcctgcac cttcgctttg gaacgatcag catccgggcg ctggcgcggg aggcggccgg
      121 gaccagtgat acttttctca acgaactgat ctggcgggac ttttaccaga tgatcctctg
      181 gcattttcca tgggtaggcg agggtagggc ctttaaaccg gcttatgacc gcatcgaatg
      241 gcgtaacaac gaggccgagt ttgaaaaatg gtgcgccggg cagaccggtt atcccatcgt
      301 cgatgccggc atgcgtgaac tgaatgccac gggattcatg cataatcgcg tacggatgat
      361 cgtcgcttcc tttcttgcca aacacctgct gatcgactgg cggtggggag aagcctattt
      421 tgccgggaag ctcctggatt tcgacctggc cgccaataat ggcggctggc aatgggcggc
      481 aggcagcggc tgtgacgcgg ccccctattt ccgggtattt aacccgaccc tccaggcgaa
      541 aagattcgat cccgatcacc ggtatatccg ccactgggtg cccgagtggg aagaactcac
      601 ctacccgcgg ccgatcgtcg aacatgaaat ggcccgaaag cgggcgctgg aggtctatag
      661 ccgggccgtc aaagagaagt agtttctacc cgctggtgga tccgcgcgta ggtctgaagc
      721 agggtctcga tcgctttttc tccctcctgc cccagcgtaa gcgagtaatg gttgacatag
      781 aggtcgatat gctggcgcat cacccgctcg tccatttcct gtgcatggga acgaacgaat
      841 tccggcagtt ccggataacg gtcgaacgca tattccaggc tcttccggat aaggctgttt
      901 atctttttcg acagggtggc gtcagctcct ttacggaata cgatcccgcc gagcgggatg
      961 ggcgccccgg tctcgcgttc ccagtattca ccaaggtcca ataatttgac caatcccttt
     1021 tgctggtaag tgaaccggtt ctcatgaatg atcacgccgc aatccaccgc gccggacaag
     1081 acggcatctt cgatgccgga gaagaccatg aactcttttt ttcgggcgtc cggaaatgca
     1141 agggagaaaa ggagatgggc tgtcgtccgc tgaccgggga tcgcgatccg gcaactgttg
     1201 atgtccgttg aagataccgg ctggcgggca ataagcaagg ggcctactcc tttccccaat
     1261 gcgccgccgg catccagcag atcgtaccgg ggtaatacca gcggcaatac gccgtagctt
     1321 attttcgaga tatccagctt cccttgcatc gcccactggt tgagcgtttc gacatcctcc
     1381 aggaagagct cgaagtcgat tccctccgta tcgatctttt tgttgacgag ggcatcgaag
     1441 ataaaggtgt cattcgggca gggactgaat cccagggaaa atttcatgct ttatttattt
     1501 tttccaattt ctccaacaac aggatcagtt cttcatttag ccgggcgata gccaggggga
     1561 tatcccattt ggatttgtcc cgtatgccga cggcattgga caccgaccgc aattgcagaa
     1621 agggaacacc cgcctgcaga cagacataat gcatcgggcc tccttccatg ctttcaacaa
     1681 cggccgccgt attttgttga tggtagccga tccttttggc atcagtcgtt atttcattga
     1741 cagtcatacc cctcacgggc tcgagaccgg tgagggtaag cagttcgcca taggggttga
     1801 caagggcgcc gccggcaaaa ggaaaatgat ccggctctgc cagtcgcata tcgaacaagg
     1861 ttttaaaccg gcctttctcc cagactccca gatcggccaa aagctcctcc ctgacgacaa
     1921 acacttcgcc atacttcctg ccggtaaaac aaccggcgat gccggcttgc aggacaagtc
     1981 ccggccggcc cctgtcaatc tgtcgggtta gcgcccaggt ggtggcgagg ccgcctacac
     2041 ccgtgatcag cggcgttacc gaatgacgcc tgtcttttcc cgtcagctcc cggagataat
     2101 cgatagtggg ttgaatttca aacgttgtcg cagccaccag caggatttcc atcctacaaa
     2161 tatctgcttc ttaattgtta cctttgcggg aattttatcg ttaatacaga ctaggagaaa
     2221 gaaatggtgt atctgacacg cctggaacat ttcaatgcag cacataaact ctacaatcca
     2281 ggctggacca gggaaaagaa tgagagcgtc tttggcaaat gcgccaatga gaattggcat
     2341 ggacataact atgaattgta tgtgaccata aagggcgaac ccgatcccga tacggggttc
     2401 ttattcgatg tgaagacatt gggagagctc attcgcgagc atgtgatcga gaaactggat
     2461 cataagaacc tgaacctcga cgtggatttt atgcgggata aaatgtgctc caccgagaac
     2521 ctggccatcg gcatctggca ccaactgctg ccgcacctgc ctgccggggt ccggttgcat
     2581 gggatcaaac tctatgagac accgaggatc tatgttgaat attttggctg acaagaccca
     2641 aaaaaataaa gcatgagcgg gaacgaaaga aaagtcgcgg ttttccggaa agaacatttc
     2701 aatgcggcgc atcgcatttt caatcccgcc tgggatgacc ggaagaatgc cgaagtgttt
     2761 ggcaagtgca gtaatccgaa ttatcatggc cataactatg aactgatcgt caaggtgacg
     2821 ggcgtgcccg atcccgagac ggggtatgtt atggatatga aggtgttgtc gcggatcacc
     2881 gctgaagagg tgctggagaa attcgatcat aagaacctca acctcgacac ggtctacttc
     2941 cgcgatctca acccgacggc cgaacacatt gcgatggtga tctatgatct gcttcgtgcg
     3001 aagatagaac cccggctgga tttacaggta aggttgtatg aaacggaacg gaattttgtg
     3061 gaatacccgg cctgaggaac ggtaaaaata atc
//
LOCUS       DATGFG010000821         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_814314, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000821 DATGFG010000000
VERSION     DATGFG010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814314"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2272)
                     /locus_tag="VKQ52_15510"
     CDS             complement(<1..>2272)
                     /locus_tag="VKQ52_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HLZ88662.1"
                     /translation="PTGARTLYAGINGRGVFKSNDGGRNWTQILSGTTPVVAAAIGAG
                     NGFSKTIVALAPPTLPPSPAGIQVLYVTLQGTGAAPDPVGLFISKDQGNTWTQQTATG
                     MPTRTQGGYSFHMAVDPASPGDGINDIIYFGTVGQAKSVDSGATFSSIPVPHADTHAW
                     AFVLRPGLSSIVYCGNDGGIDRSLDGGATWNALGGGGLQTGLFYNIDIRPDAAGSVTV
                     GALQDNEIETTKGAPGLGWNATEGGDGWDVAYDSHIAGQVYGSSGFWSPAPCTRVHRS
                     TDDGSTFGTEITPWTTATDTGCFLAPVTTDPSTGGILYVSGSQNLWQSLTGGGSWRIL
                     SPFPGTGNVDVAPANGNNVVIAVGSRVFLSTNALLPTVGLPAGVTFTEITRNLPGRNV
                     ARACFDPTDPTIIYAVLGGFSGIASGPSKHVFRTTVGASSWTDISPDLDLPCNALALD
                     GTDTPTTIYVGTDFGVLRSVDIGATWYVLDDIHFPRAPVLDLIYSRGHLRAATYGRGV
                     FQFVLPTGPAIAVDLPEGLAFGTVCKGPAFQLLKIYNVGVSDLIIFSVQRLMGSTAFS
                     VAGTPGTPLIISPGEEIDFTIQYTPVSTGSTDTATIRISSNDPGAPFVDLAVTGTKGT
                     GRLETAIADSGSFGNVCLGSFADELLTINNSGPCPLSITNITSSSADFKAPGVTAYPL
                     VVSPGGSLDVMIRFQPTAFGAAFATITISSDDPASPHTIAVSGIGQQPRQVLMIADSG
                     NFGNCCIGSFVDKPLTV"
BASE COUNT          440 a          639 c          763 g          430 t
ORIGIN      
        1 gacagtgagc ggcttgtcta caaaggagcc gatgcagcaa ttgccgaaat tgcccgagtc
       61 ggcgatcatg aggacctgcc gcggctgctg gccaatcccg gaaacagcga tcgtgtgagg
      121 gctggcggga tcgtcgctcg agatggtgat cgttgcaaag gcggccccga aagccgtggg
      181 ttgaaagcgg atcataacat ccagggagcc acccgggctt accacgaggg gataggcggt
      241 gacacccggg gccttgaagt cggcggaaga ggaagtgatg ttggtgatcg acagggggca
      301 gggtccgctg ttattgatgg tcagcagttc atcggcaaaa gaaccgagac agacgttgcc
      361 gaagctgccg ctgtcggcga tggcggtctc cagcctgccg gtgccctttg tgccggtgac
      421 ggcgaggtcg acgaagggcg cccccgggtc gttgctgctg atgcggatgg ttgccgtgtc
      481 tgtacttccc gtcgagactg gcgtatattg gatggtgaag tctatttctt ctccggggct
      541 tattatcagt ggcgtcccgg gtgtcccggc aaccgagaaa gcggtggacc ccattaatcg
      601 ctgtacgctg aaaatgataa gatcggatac accgacatta tagatcttca ggagctggaa
      661 ggcgggtcct ttgcataccg ttccgaaggc caatccttcc ggcagatcga cggcaattgc
      721 gggacccgtc ggcagtacga attgaaagac accgcggcca taggtagcgg cccgaaggtg
      781 gcccctgcta tagataaggt cgagtaccgg cgcccgtgga aaatggatat cgtcgagcac
      841 gtaccatgtc gcgccaatat ctacagagcg cagcacgccg aaatcggtgc ccacatagat
      901 cgtcgtaggg gtatcggtgc cgtccagggc gagggcattg cagggcaggt cgagatcggg
      961 tgagatgtcg gtccaggaag aggcaccgac ggtcgtcctg aaaacatgtt tggagggacc
     1021 ggatgcgatg ccgctgaacc cgcccaatac ggcgtagatg atagtgggat cggtggggtc
     1081 gaagcatgcc cttgccacat tcctgccggg gaggttgcgg gtgatctcgg tgaaggtcac
     1141 gccggcggga aggcctacag tcggcaacag ggcgttggtg gacaggaata ccctgctgcc
     1201 tacggcaatc acgacgttgt tgccattggc cggcgccaca tcgacattgc cggtgcccgg
     1261 aaagggcgag aggatgcgcc aactgccgcc gccagtgagg ctctgccaaa gattctggct
     1321 tccgcttaca tagaggatgc cgcccgtcga gggatcggtc gttaccgggg ccagaaagca
     1381 gcccgtgtcc gtggccgtcg tccagggggt gatctccgtc ccgaaagtcg agccgtcatc
     1441 ggtagagcga tgcacgcggg tacagggagc cggcgaccaa aacccgctgc tgccatatac
     1501 ctggccggcg atgtgggaat cataggccac gtcccagcca tccccacctt ccgtcgcgtt
     1561 ccaacccagg cccggggcgc cctttgttgt ttcgatctca ttgtcctgga gggcgccgac
     1621 cgtcacgctg ccggcggcat ccggccggat gtcgatgtta tagaaaaggc cggtctgcaa
     1681 gccacctccg cccagggcgt tccaggtggc gccgccatcg agggatctgt cgatcccgcc
     1741 gtcattcccg caatacacga tcgatgaaag gcctggccgg aggacgaagg cccaggcatg
     1801 cgtatcggca tggggcacgg ggatggagga gaaggttgcg ccggagtcga cggacttagc
     1861 ctggcccact gtaccgaagt aaatgatatc gtttatcccg tcaccgggtg atgccgggtc
     1921 gacggccata tgaaagctat agccaccctg ggttcgggtg ggcatccccg tggctgtctg
     1981 ttgggtccag gtgttgccct ggtccttgct gatgaacaga cctaccggat cgggcgcggc
     2041 gcctgtccct tgcagcgtca cgtagagcac ctggataccg gccgggctgg ggggtaatgt
     2101 cggcggggca agggcgacga tcgttttact gaatccgttt cccgccccga tggccgccgc
     2161 gaccaccggc gtggttccgc taaggatctg tgtccagttg cgccccccgt cattggattt
     2221 gaaaactcca cggccgttga tcccggcgta caaggttctg gcgcccgtgg ga
//
LOCUS       DATGFG010000822         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1526940, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000822 DATGFG010000000
VERSION     DATGFG010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526940"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VKQ52_15515"
     CDS             complement(<1..886)
                     /locus_tag="VKQ52_15515"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ88663.1"
                     /translation="MDQHYDTSPEDLLVKYLLGETSREETRTVEEWLAASPGNQHYYQ
                     QVKLIWEKSLLLPAGTLRESDDDEEKAWRIFKEKIHRPARPRPIISLRWAATAAAAVI
                     LLPILFIALFGWPGSRTMQVLSSNNSILPDTLPDGTTVILSRHSTLSRQKKFNRRTVA
                     LQGEAWFRVATNKDRPFQIASNGITITVLGTSFNVRTDPAETDITVETGLVKVSNTHG
                     ATEVGAGETITIRPDDQRLQKHPAAPAIYHFYQPREFVCRDTPLWQLTDALQRSYGVP
                     IILGDTTLRQLRISTSFHN"
     gene            complement(897..1535)
                     /locus_tag="VKQ52_15520"
     CDS             complement(897..1535)
                     /locus_tag="VKQ52_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HLZ88664.1"
                     /translation="MLSPKEDLITIATCKAITQPLTMEWDDAETIRLLKARNEEVFGQ
                     LFKAHFKPLRKYAFTFVQQQQLAEDIVQTIFYKLWQRVEVLNFSDSVAAYLYRAVYNE
                     SLNALKHSKVKRVYHSWLHRNMKHQTDTANKKSQHDELERRLHTAINELPQQCRTIFQ
                     LSRFEGLRYQDIGHRLGISPKTVENQMSKALRILRARLIEFLPLLALYFINH"
     gene            complement(1575..1772)
                     /locus_tag="VKQ52_15525"
     CDS             complement(1575..1772)
                     /locus_tag="VKQ52_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88665.1"
                     /translation="MDNRNSSIIYGILGLYSLMISWHYNHSLILLIIHYIFWPIYLIY
                     ELLIGHLSHDLWRTIPLSYFK"
BASE COUNT          381 a          446 c          665 g          514 t
ORIGIN      
        1 cgttgtggaa gcttgtgctg atccggagct gccggagggt ggtgtcgcca aggataatgg
       61 ggacgccata ggatctttgc agggcatcgg tcagttgcca gagcggggta tcccggcaga
      121 cgaattcccg gggttgataa aagtgataga ttgcgggggc ggcgggatgt ttttgcagcc
      181 gctggtcatc cggcctgatc gtgatcgttt cacccgcacc gacttcggtt gcgccgtggg
      241 tgttggacac ttttacgagc ccggtctcga cggtgatgtc ggtttccgcc gggtctgttc
      301 tgacgttgaa ggaggtgccc agcaccgtga tggtgatgcc gttggaggct atttgaaagg
      361 gacggtcctt gttggtggcg acccggaacc aggcttcgcc ctgcagggcc acggtgcggc
      421 ggttgaattt tttttgccgg gagagggtcg agtgccggct gaggatgacg gtggtgccat
      481 cgggcagggt gtccggaagg atgctgttat tggaactgag gacttgcatt gtccggctgc
      541 cgggccagcc gaagagggcg atgaagagga tgggcaggag gatgacggct gccgctgcag
      601 ttgccgccca gcggagggaa atgatgggtc ttggcctggc cggcctgtgg atcttttcct
      661 tgaagatccg ccaggctttt tcttcgtcgt catcggattc gcggagggtg ccggcgggca
      721 gcaggaggct tttctcccag atgagtttca cctgttggta atagtgttgg ttacccgggg
      781 atgcggccag ccattcttcc acggtgcggg tctcttccct gctcgtttcg ccgagcaggt
      841 atttgaccag caggtcttcg ggtgatgtat cgtagtgttg gtccaagtgc gtggatttag
      901 tggttgataa aatacagggc cagcagcggc aggaattcga tgagtcttgc ccggaggatc
      961 ctcagggctt tgctcatctg gttctcgacc gtctttggag agatgccgag gcgatggccg
     1021 atgtcctggt agcgcagccc ttcgaagcgg ctcagctgga agatagtgcg gcactgttgc
     1081 ggcagttcgt tgatggccgt gtgcagccgt cgttccagtt cgtcgtgctg tgattttttg
     1141 ttggcggtat ccgtttgatg cttcatattc cggtgtaacc aggaatgata gacccttttc
     1201 accttgctat gttttaaggc gttgaggctt tcgttgtaaa cggcccggta gaggtaggcg
     1261 gcgacggagt cggagaagtt caggacctcg accctttgcc acagtttgta gaaaatggtc
     1321 tggacgatgt cttccgccag ttgctgttgc tggacgaagg tgaaggcata tttgcgaagc
     1381 ggcttaaaat gggctttaaa cagttgcccg aatacttcct cgttcctggc tttcagcagc
     1441 cggatggttt cggcgtcatc ccattccatg gttagcggtt gtgtaatggc tttgcaggtg
     1501 gcgatggtta tcaaatcctc tttaggggat aacaatccta ttggaagtta cccctatgcc
     1561 ggcaacaaaa aattctattt aaagtaggac agggggatgg tgcgccacag gtcgtgggaa
     1621 aggtgaccga tgagcagttc gtagatgaga tagatgggcc agaagatata atggatgatc
     1681 agcaggatca gcgagtggtt gtagtgccaa ctgatcatca gggagtagag gcccaggatg
     1741 ccgtagatta tgctggagtt tctgttatcc ataatagtct aaactaagca aaaaaggggc
     1801 caccttcaaa gaaaagtagg cggccccttt tttcacttat gtccggcaaa taaatttgcc
     1861 gggggagggg ccacgggggc cccttttgtc ctatttaggc gtttatttac agatggatgg
     1921 cttcgccata ggcggcttcg actgcgtcct ttacggcctc actgatggtg gggtgcgggt
     1981 ggacggcgtc gaggacctcg tgatag
//
LOCUS       DATGFG010000823         6227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_384323, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000823 DATGFG010000000
VERSION     DATGFG010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384323"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VKQ52_15530"
     CDS             complement(<1..647)
                     /locus_tag="VKQ52_15530"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88666.1"
                     /translation="MAKRIWAERRRRWMEAIKNEPGGMRRFADLVAKLGTSTKTNDKL
                     SALDRYFIEAADADKVWMIALFSGRRPKRTVTTTQLWEWCREITGLPAWLFEESYHTV
                     GDLAETIALLLPESTAVVAESESLAYYLLELMQLGKKEEVEKKAFVQRCWASMNKEER
                     FVFNKLITGGFRIGISQQTVVNALAKTAGVDAAVVAHRISGDWDAATTPFAALLSG"
     gene            complement(589..1626)
                     /locus_tag="VKQ52_15535"
     CDS             complement(589..1626)
                     /locus_tag="VKQ52_15535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HLZ88667.1"
                     /translation="MPLIEFTDKGLFCSQGQFYVDPWRPVDKAVITHAHSDHARPGSG
                     SYLCHRLTRALLDLRLSVEGIRPNVQAVEWGVPVYLNGVRVSLHPAGHVIGSSQVRVE
                     YKGEVWVVSGDYKTEDDGISGAFEALPCRVFITESTFGLPIYHWKPQAELFAGMRDWV
                     AENKAQDRTSVLIAYSLGKAQRLLKGLEPMGGKVFVHGAVWNVHQALVHAGVELPPVE
                     RVLPDMPKELFRGQVIIAPGSAEDSPWMRRFSPYAIGICSGWMQVRGNVRRRNADLGL
                     AMSDHADWKGLLSAISATGAEKVFVTHGFQSTLTRYLNEQGIAAAEVKTEYGEEDLGG
                     EEAPVDGSDKE"
     gene            complement(1628..2794)
                     /locus_tag="VKQ52_15540"
     CDS             complement(1628..2794)
                     /locus_tag="VKQ52_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88668.1"
                     /translation="MLFLSLGAGAQVDTTLHPADSSRHRIAIFTPLYLDSAFDAAGGY
                     RYDKNFPKFINPGLEFYEGAQMAFDTLQKEHVALDVQVYDTRATGVTLQQQLDSAAAQ
                     GMELLIGQVNNATELQQMAAAALRKNIPFINVNYPNDGGITNNPSLVVLNSTLRAHCE
                     GIYRYLQRNYPTRPLIYFRKKGTQEDRLKGYFTDLDKKTAGVPLKIKYVTLDDNFDAA
                     ALAANMDSSVSQTVCISGSLDDEFASKLCAQLAYLNKSYHTTVIGMPTWDNLDLARPE
                     YAGEEITYTTPFYLNPADTLVKIITQNFKTKFYSRPSDMVFRGYEAAYRFAKLLLLHG
                     KALSGSIGEKRFKVFNDLEITPVFLNRQTMTLDYFENKKLYFIRKMDGNVVGVY"
     gene            complement(2820..4352)
                     /gene="guaA"
                     /locus_tag="VKQ52_15545"
     CDS             complement(2820..4352)
                     /gene="guaA"
                     /locus_tag="VKQ52_15545"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HLZ88669.1"
                     /translation="MTEKILILDFGSQYTQLIARAVREANVYCEIIPFQKSISFDPSL
                     KGIILSGSPFSVNDANAPEVDIAALHSRLPVLGVCYGAQLTAKQFGGRVANSNKREYG
                     RALLHKRKDSELLKDVTELSQVWMSHADTILDLPAGFEVLANTENIPYAAFMKGEGEQ
                     PLYCLQFHPEVYHSVEGKKIIRNFLVNVCGCHQDWTPAHFVSDTIDHLKKQIGGRKVI
                     MALSGGVDSTVAATLIHRAIGDRLVGIFVDNGVLRKGEFEQVLATYQQIGLNVKGIDA
                     KKLFYSELAGKADPEAKRKTIGRLFIEVFQDEAKKVAGVELLGQGTIYPDVIESVSVH
                     GPSVTIKSHHNVGGLPEKMHLELVEPLRFLFKDEVRRVGAELGIPEDLLQRHPFPGPG
                     LAIRILGEVTEEKVRLLQEADHVYTKGLKDHHLYSSVWQAGAILLPVKSVGVMGDERT
                     YEFTVALRAVTSVDGMTADWAHLPYTFLADISNDIINSVKGINRVVYDISSKPPATIE
                     WE"
     gene            4409..5152
                     /locus_tag="VKQ52_15550"
     CDS             4409..5152
                     /locus_tag="VKQ52_15550"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HLZ88670.1"
                     /translation="MRNAFPTLRSACLVLALPLLLASCQEFMGKRVHGNGNIRTEDRP
                     VNDFKNVEVGGAAKVMVSQGDHSSVKLEGDENLLQYMEVTQEGDKIYIREKRGFHLLP
                     THDLRVYVTAPVFNSIDASGACDILGQTKISNPENLEMHVSGAGDIKMEIDAPRVRAE
                     VTGSGSIYLKGQTKDVDLDLTGAGHAHCYELLSENTKVDISGAGSAEVYASVKLDAQV
                     SGAGDVNYKGNATTVNQHVSGAGSVHKAD"
     gene            5320..5868
                     /locus_tag="VKQ52_15555"
     CDS             5320..5868
                     /locus_tag="VKQ52_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88671.1"
                     /translation="MTCRIPALILASIISLAARPQQSPTGAALPNPEEFVNAVYPTIV
                     DSSFQQYYLVIGTDSCRFLKYDYDEWIKYHLREPLSFTIMNELSQKVYLSRYPYFWKQ
                     ARLQKAVCITRKQADSLLEDKKDVVFSFSLPQFTDNGEYAVIDLNLLCGARCGQSITC
                     IFRLTTTGEWKLVGRNINWTSF"
     gene            complement(5872..>6227)
                     /locus_tag="VKQ52_15560"
     CDS             complement(5872..>6227)
                     /locus_tag="VKQ52_15560"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88672.1"
                     /translation="PEMKEYYDANRITYQNIPPHNPNCEKVFREGAPVVMSPANGSEY
                     LINRKDPEPLLLRCRVSNDVGRVYWYINDKFYKSGEAGSREFFVPDEGPVKISCTDDK
                     GRNRNVWIRVRYVNL"
BASE COUNT         1370 a         2001 c         1513 g         1343 t
ORIGIN      
        1 ccgctaagca gtgctgcaaa aggtgtcgtc gccgcatccc agtccccgct gatccggtgt
       61 gcaaccaccg cggcatcgac cccggcggtc ttcgccagcg cattcaccac ggtctgctgg
      121 gagatgccga tcctgaatcc acccgtgatc agcttgttaa agacaaaccg ctcctcctta
      181 ttcatcgaag cccagcaccg ctgcacgaag gccttcttct ccacctcttc tttctttccc
      241 aactgcatca actccagcag gtaatacgca agcgattcac tctcggccac caccgccgtg
      301 gactccggca gcaacaacgc aatggtctcg gccagatcac ccaccgtatg ataactctct
      361 tcaaaaagcc acgcgggcaa tcccgtgatc tcgcggcacc actcccacaa ttgcgtggtc
      421 gtcaccgtgc gcttcggccg gcgaccgctg aaaagcgcga tcatccacac cttatcggca
      481 tcggcggcct cgatgaaata acggtccagc gccgacagct tgtcgttggt ctttgtactg
      541 gtgcccagct tcgccacgag atcggcaaac cggcgcatcc cgcccggctc attctttatc
      601 gcttccatcc accggcgcct cctctccgcc caaatcctct tcgccatatt ccgtcttcac
      661 ctccgccgcc gcgatgccct gctcattgag ataacgtgtc aatgtggact ggaagccatg
      721 tgttacgaac actttctccg cacctgtcgc gctgatcgcc gagagcagac ccttccagtc
      781 ggcatgatcg ctcattgcga gtcccaagtc ggcattccgc cgccgcacat tcccccgcac
      841 ctgcatccag ccgctgcaga tcccgatggc atagggactg aaccgccgca tccacggcga
      901 atcctccgca ctccccggcg caatgatcac ctgcccgcgg aaaagttcct ttggcatatc
      961 gggtaacacc cgctccaccg gcggcagctc caccccggcg tgtacaaggg cctggtggac
     1021 attccacacc gcgccatgga caaagacctt cccgcccatc ggttccagac ccttcaacaa
     1081 ccgctgcgcc ttgccaagac tgtaggcgat cagcacggag gtcctgtcct gtgccttgtt
     1141 ctccgccacc cagtcccgca ttccggcaaa cagctccgcc tgcggcttcc agtgatagat
     1201 cggcaaccca aatgtcgact ccgtgataaa aacccggcag ggcagggcct cgaaggcgcc
     1261 gctgatgcca tcatcctcgg tcttgtagtc tccgctcacc acccagacct cgcccttgta
     1321 ttccacccgc acctgtgaag acccgataac atgccccgcc ggatgaaggc tcacccgcac
     1381 cccgttcagg tacacgggca caccccattc cactgcctgg acattgggcc tgatcccctc
     1441 cacgctcagt cgcagatcca gtaaagcccg ggtaagccgg tggcagagat agctgccgct
     1501 ccccggccgg gcatgatcgc tatgggcatg cgtgatcacg gccttgtcca ccggtcgcca
     1561 ggggtcgaca taaaactgcc cctgggaaca gaataatccc ttatcggtaa attcgattaa
     1621 aggcatacta atacactccc actacattcc catccatctt ccggatgaag taaagctttt
     1681 tgttctcgaa gtagtccagc gtcattgttt gccggttgag aaacacgggt gtgatctcca
     1741 ggtcgttaaa gaccttgaac cgcttctccc cgatactccc gctaagcgcc tttccatgca
     1801 acaacaacag ctttgcaaaa cggtaagcgg cctcgtaccc ccggaacacc atatcgctgg
     1861 gacgggaata gaactttgtt ttgaaattct gcgtaatgat ctttaccagg gtatccgcgg
     1921 ggttaagata aaaaggcgtg gtataggtga tctcctcgcc ggcatactcg ggacgtgcaa
     1981 gatcgagatt gtcccaggta ggcatgccga taacggtcgt atggtaggat ttgttgagat
     2041 aagcaagctg ggcgcaaagt ttggatgcaa attcatcatc cagcgaaccg gagatacaca
     2101 cggtttgcga aacactgctg tccatattgg ccgccagcgc ggccgcatcg aaattatcgt
     2161 ccagcgtgac atattttatt ttcagcggca ccccggccgt cttcttgtcc agatcggtaa
     2221 aatatccctt cagccggtct tcctgtgtgc ctttcttccg gaaataaata agcggcctcg
     2281 tgggatagtt ccgctggaga taccggtaga tgccttcgca atgcgcccgt aaggtggaat
     2341 tgaggacgac gaggctgggg ttgttggtga tcccgccgtc attgggatag ttgacattga
     2401 taaaggggat attcttccgc aatgcggccg ccgccatctg ctggagttcc gtggcgttat
     2461 tcacctgccc gatcagcagt tccatcccct gcgcggccgc gctgtccagc tgttgctgca
     2521 gcgtgacgcc tgtcgcccgg gtgtcataga cctgcacgtc cagcgccaca tgctcctttt
     2581 gcagggtatc gaacgccatc tgcgccccct catagaattc cagacccgga ttgatgaatt
     2641 tgggaaaatt cttgtcgtag cggtaaccgc ccgcggcatc aaaggcggag tcgagataaa
     2701 gcggggtaaa gatcgcgatc cggtgccggg aactgtctgc cgggtgcagc gtcgtgtcca
     2761 cctgggcgcc ggcgcccagg gataaaaaca acaaaaaata tagtaatgcg cttttcatct
     2821 tactcccact cgatcgtcgc cggcggcttg ctgctgatat cgtagaccac ccggttgatc
     2881 cccttcaccg aattgatgat atcattggag atatcggcca ggaaggtata cggcagatgc
     2941 gcccagtcgg ccgtcatccc gtcaacggag gtcacagccc gcaaggccac tgtaaactca
     3001 tatgtccgtt catctcccat cacccctaca cttttcaccg gcaacaggat cgcgcccgcc
     3061 tgccagaccg aggaataaag atgatgatcc ttcagcccct tggtatagac atgatcagct
     3121 tcctgcaata atcgcacctt ttcttccgtg acttccccca ggatgcggat cgccagtccc
     3181 ggccccggaa agggatgccg ttgcaaaaga tcctcgggga tccccagttc cgcccccacc
     3241 cgccttacct cgtccttgaa caggaaccgc aacggctcca cgagttcaag atgcattttt
     3301 tccggcagac cacccacatt gtgatgggat ttgatcgtca ccgaagggcc atgcaccgaa
     3361 acgctttcga tcacgtcggg gtagatcgta ccctgtccca acaattccac acccgctact
     3421 ttcttcgcct cgtcctggaa tacctcgata aagagccggc cgatcgtctt tcgcttcgcc
     3481 tccggatcgg cctttccggc cagctcggaa taaaataatt ttttcgcatc gatgcccttc
     3541 acgttaaggc ctatctgctg gtacgtcgcg agcacctgct cgaattcccc cttgcggagg
     3601 accccgttgt cgacaaagat ccccaccagc cggtcaccga tcgcccggtg gatcagcgtc
     3661 gccgccaccg tggaatccac gccgccgcta agcgccatga tcaccttccg cccgccgatc
     3721 tgctttttca ggtggtcaat ggtgtcgctg acaaaatgtg ccggcgtcca gtcctggtgg
     3781 caaccgcaaa cattgacaag aaaattccgg atgatctttt tgccctcgac ggaatgatag
     3841 acttcaggat ggaattgcag acagtacagg ggctgctccc cttcgccctt cataaaagcg
     3901 gcataaggga tattctccgt attcgccagc acttcgaaac ccgccggcag atccaatatc
     3961 gtatcggcat ggctcatcca cacctgtgac agttcggtaa catccttcag cagctcgctg
     4021 tcttttcgct tgtgcaacag cgcccgcccg tattcccgtt tattcgaatt ggccacccgg
     4081 ccgccaaact gtttggccgt cagctgggcg ccataacaca cgccaagcac cggcaaccgg
     4141 ctatgcaggg cggcgatatc cacttccggc gcattggcgt cgttcaccga aaaaggcgac
     4201 ccggacagga taataccctt gagcgacggg tcaaagctga tggatttttg aaaggggatg
     4261 atctcacagt atacattcgc ctcacgtaca gccctggcga tcaattgagt atattgggag
     4321 ccaaagtcaa gaattaagat cttttccgtc atggtgacgc gaagttaccg cagaaaatcc
     4381 taattttcgc gtacctaaaa caaacaaaat gagaaacgcc tttccaacac tccggtctgc
     4441 ctgccttgtc ctcgccctcc cgctgttgct cgcttcctgc caggaattca tgggcaaacg
     4501 cgtacatggc aacggcaata tccggacaga agatcgtccg gtcaacgact tcaagaatgt
     4561 agaggtcggc ggcgcggcaa aagtcatggt ctcccaggga gatcactcct ccgtaaaact
     4621 cgagggtgac gagaatctcc tccagtatat ggaggtcacc caggaaggcg acaagatcta
     4681 tatccgggag aagcgtggtt tccatcttct ccccacccac gacctccgcg tctatgtcac
     4741 cgccccggtg ttcaactcca tcgacgcctc gggcgcctgt gacatcctcg ggcagacaaa
     4801 gatctccaat cccgaaaacc tcgagatgca tgtcagcggg gcaggggata taaaaatgga
     4861 gatagacgcc ccccgtgtcc gggccgaagt caccggcagc gggtccatct atttaaaagg
     4921 ccagaccaag gacgtcgacc tggacctcac cggcgcaggc catgcacact gctatgagct
     4981 tctctcggaa aatacaaagg tggatatctc cggcgcgggc tcggcggaag tctatgcaag
     5041 tgtaaaattg gatgcgcagg taagcggagc gggagatgtc aactacaaag gcaacgccac
     5101 cacggtaaac cagcatgtga gcggcgccgg cagcgtacac aaagcggatt gaagacggac
     5161 cacaggtccg ccggccgaag gcgtcggcag catatttaga caaacgggag gccctagccc
     5221 cggcaacccc ataatcattt acttatcatt gagcgctcgg cctacgccgg gcgctcccgt
     5281 tttagtaact ccttaacgta gttttttcga tcttagtgta tgacctgcag aattcccgcc
     5341 ctcatcctgg cctcgatcat cagcctcgcc gcccgcccgc agcaatcacc cacgggcgcc
     5401 gccctcccca atcccgaaga atttgtcaac gccgtctatc ccactatcgt cgacagctcc
     5461 tttcaacaat actatctcgt catcggcacc gactcctgcc gcttcctcaa atatgactat
     5521 gatgaatgga taaaatacca cctccgggaa cccctctcct tcacgatcat gaacgagctc
     5581 tcgcagaagg tctatctctc ccgttatccc tatttctgga aacaggcccg gttgcaaaaa
     5641 gcggtctgta tcacccgtaa gcaggccgac tcgctcctgg aggacaaaaa agatgtcgtc
     5701 ttcagctttt cccttccgca attcaccgat aacggtgaat acgccgtcat cgatctcaac
     5761 ctcctctgcg gcgcccgctg cggtcagagc atcacctgca tcttccgcct caccaccacc
     5821 ggcgaatgga aactcgtcgg caggaatatc aattggacct ccttctgatc cctacaggtt
     5881 cacataccgc accctgatcc atacattcct gttcctcccc ttgtcatccg tacacgagat
     5941 cttcaccggc ccttcgtcgg gaacaaagaa ctcccttgag cccgcttccc cgctcttgta
     6001 gaacttatcg ttgatatacc agtaaaccct ccccacatca ttgctcaccc ggcaacgcag
     6061 cagcaggggc tcgggatctt tccggttgat caggtactca ctgccgttag ccggtgacat
     6121 caccaccggc gctccctcgc ggaacacctt ttcgcaattg gggttgtgag gggggatatt
     6181 ctgataggtg atccggttgg catcatagta ctccttcatc tccggcg
//
LOCUS       DATGFG010000824         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_610204, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000824 DATGFG010000000
VERSION     DATGFG010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610204"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VKQ52_15565"
     CDS             <1..204
                     /locus_tag="VKQ52_15565"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HLZ88673.1"
                     /translation="PPAGFTAVVCLSESHISVHTWPEHGRVNLDIYLSNHLRDNDGTT
                     RALYEKLRCWFDAEILQEQTLKR"
     gene            210..2129
                     /locus_tag="VKQ52_15570"
     CDS             210..2129
                     /locus_tag="VKQ52_15570"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HLZ88674.1"
                     /translation="MGKETGMYQCPLCSKAIVFRSKDTRLKVCACGSVLNRLESDDLA
                     LKPAFIIPDHNDLLQIGATGVWQGRSFEIIGRYRVWLAESVYNYWTILFDDGPAILAE
                     GYGMYAILQRMTPEENITAWDVSRLDIADSVNLAANIPWYLQRRDKAWKYEVEGEVWM
                     PECTDQFTIYDFYARGDHHAEAIEFLNNYIVFYSISFTEFSDLQLTNLNESPVTPKEV
                     NCTECNTTIEVKTFPYAQSCSCPGCGTRFAFRGAGAGFRSLKKDKENDNAASIALGSK
                     GRIRNIDYEVIGYALKEEDNAEAARWKEYVLYNRAEGYAFLSEYGGSWLYARERGNSP
                     VIGSNSPDRIFYKGSEYDLYNRYSIRIVDTAGEFPYDIFDDERKIVSAEFIAPPHMWI
                     YEKSSAEGINWFLAEYQDRKELEQQFPDAAIPAQTEMGVLDPKGRIDPALLIKATLAG
                     IIFLTLVHFLIGATQQQRTVFEGDLQLRDSAASSTFVSPQFSLHKWRSNLRFDITSPV
                     DNNWIDMEAILVNATNGQEYGLQQTVEYYHGVDDGDSWNEGSTHETAFLSSLPSGDYY
                     LRIQASRDTTSAGWDAVKDLTVTVKNDVPMHRNLFIFLGILLVWPVIAYIWYYINERR
                     RWKNSAYSPYNTKNE"
     gene            2122..2304
                     /locus_tag="VKQ52_15575"
     CDS             2122..2304
                     /locus_tag="VKQ52_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88675.1"
                     /translation="MNNPFRPGSSWYPIRWFLVLIATLTMGMTYVDVTGLRFLSSAAN
                     QQPQRGGYYGTHYYHK"
     gene            2311..>2559
                     /locus_tag="VKQ52_15580"
     CDS             2311..>2559
                     /locus_tag="VKQ52_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88676.1"
                     /translation="MSKPNSAQWLLLVAVFVIATCGLIYELVAGTLASYVLGDSITQF
                     STIIGVYLFSMGVGSWLSKYFENNLLRWFIQIELLVGLV"
BASE COUNT          596 a          807 c          662 g          494 t
ORIGIN      
        1 ccgcctgcgg gcttcaccgc cgtcgtctgt ctcagcgaaa gccatatctc cgtacatacc
       61 tggccggaac atggccgggt gaacctcgac atctatctca gcaaccacct ccgcgacaac
      121 gatggcacca cgcgggccct ctacgaaaaa ctccggtgct ggttcgacgc ggagatcctc
      181 caggaacaaa cccttaaacg ataaaaccca tgggcaagga aaccggaatg tatcaatgcc
      241 cgttgtgctc aaaggcaatc gtctttcgga gcaaggacac ccgcctgaag gtctgcgcct
      301 gcggcagcgt cctcaaccgc ctcgaaagcg acgatctcgc cctgaagccg gcattcatca
      361 tcccagacca taacgatctt cttcagatag gcgccaccgg cgtctggcag ggccgctcct
      421 tcgagatcat cggccgctac cgcgtctggc tcgcggaatc cgtctacaac tactggacca
      481 tcctcttcga cgatggaccc gccatcctcg ccgagggcta cggcatgtac gccatcctgc
      541 aacggatgac accggaagaa aatatcacgg cctgggacgt ctcgcggctc gacatcgccg
      601 actcggtcaa cctcgcggcc aatatcccct ggtatctcca gcggcgcgac aaggcctgga
      661 agtacgaggt cgaaggcgag gtatggatgc ccgaatgcac cgaccagttc accatctacg
      721 atttctacgc ccgtggcgat caccatgccg aagcaatcga attcctcaac aactatattg
      781 tcttttatag tatcagcttc acggaattca gcgatctcca actgaccaac ctcaacgaaa
      841 gcccggtaac tcccaaggag gtgaattgta ctgagtgtaa taccactatc gaagtaaaga
      901 ctttccctta tgcgcaaagc tgctcctgcc caggctgtgg cacgcgcttc gccttcaggg
      961 gcgccggcgc cggtttccgt tcgctgaaaa aggacaagga gaacgacaac gcggcatcca
     1021 tcgcgcttgg ctccaaagga aggatcagga acatcgacta cgaggtcatc ggctatgcat
     1081 tgaaggaaga ggacaacgcc gaagcggcaa gatggaagga atatgttctc tacaatcgtg
     1141 ccgagggcta cgccttcctc agcgaatacg gcggcagctg gctctacgcc cgggaacgag
     1201 gcaactcccc ggtgatcggc agcaacagcc ccgacaggat cttctacaaa ggcagtgaat
     1261 acgatctcta taatcgctac tccatccgca tcgtcgacac cgccggcgag ttcccctacg
     1321 atatcttcga cgacgaacgc aagatcgtct cggcggagtt catcgccccg ccacatatgt
     1381 ggatctatga aaagagcagc gcggaaggca tcaattggtt cctggcggaa taccaggaca
     1441 ggaaggagct ggaacaacaa tttcccgacg ccgcgatacc cgcgcaaacc gagatggggg
     1501 tactcgatcc gaagggacgg atcgaccccg cgctcctgat caaggccacc cttgcgggga
     1561 tcatctttct caccctcgtc catttcctca tcggcgccac ccagcagcaa cgcaccgtct
     1621 tcgagggcga tcttcaattg cgggattcgg cggccagctc caccttcgtc tcaccgcagt
     1681 tcagcctcca caaatggcgg agcaatctcc ggttcgatat caccagcccc gtagacaata
     1741 actggatcga catggaggcc atcctcgtga acgccactaa cggccaggag tatggactgc
     1801 aacaaaccgt cgaatactac catggtgtcg acgacggcga cagctggaac gagggcagca
     1861 cacacgagac ggccttcctc agcagcctgc cctccgggga ctattacctg cgcatccagg
     1921 cctcgcggga caccaccagc gcgggctggg acgccgtgaa ggatctcacc gtcaccgtaa
     1981 agaacgatgt gccgatgcac cgcaacctct ttatcttcct ggggatcctg ctggtgtggc
     2041 ccgtcattgc ctatatctgg tattatataa acgaacgacg ccgctggaag aacagtgcgt
     2101 attcacctta caacaccaaa aatgaataat ccattccggc cgggctcgtc ctggtatccg
     2161 attcgctggt tcctggtgct gatcgccacc ctcacgatgg ggatgaccta tgtagacgtc
     2221 acgggcctgc gcttcctcag ctccgccgct aaccagcagc cgcaacgcgg cggctactat
     2281 ggcacacatt attatcacaa atagccgcgc atgtcgaaac ccaacagcgc ccagtggcta
     2341 ttactcgtgg ccgtattcgt catcgccacc tgcggactga tctacgaact ggtggccggc
     2401 accctcgcat cctatgtgct gggagacagc atcacgcagt tcagcaccat catcggggtc
     2461 tatcttttct cgatgggggt ggggtcctgg ctgtcgaaat atttcgagaa caacctgctg
     2521 cggtggttta tccagatcga attactcgtc gggctggtc
//
LOCUS       DATGFG010000825         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_298985, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000825 DATGFG010000000
VERSION     DATGFG010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298985"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..2795
                     /locus_tag="VKQ52_15585"
     CDS             81..2795
                     /locus_tag="VKQ52_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ88677.1"
                     /translation="MRTKQYCLTCFFLLFLLQAFTQTATIKGNVFDAVTRDGLPGCNI
                     AIANTAYHQVADNKGNFQLLRLPAGEYDLTVTNVGYVQLHRHITLREGQTLQLFLGLD
                     LEGKALEEVTVFGRADREKETGSRDRERTAINMMNVISSQAMVRSPDINAANVLQRMS
                     GVTIQRSNGGDEAYAVIRGMEPRYSNTLLNGIKIASPDNKNRYVQLGIVPSDILSSIE
                     ISKSLTADMEGDATGGTVNMVVRDAPDNTSFKATASVGYSQLFFDEKYVGFHTGGIQD
                     KSPVQRNPPGYTARPGDFSRSNLDFWSRQALPTTTAGFSFTHRYLHDKLGFVLSENFQ
                     NQYYGNIGFQATALPLNDTTGHLHPNDANNFKEYTQQLNNGLVAHLDYVLNDNNKISI
                     DNFYLYSYLAQARIDGDHLLIGTGGIGGNTGQVFNNYTSQTQHLYVENLKLSGQHLVS
                     PVFRLDWAGIYSTAGNRQPDVANISTVYLIQNNGVQTGTFFDGISRSWQNNDDKEYSG
                     ILNLVYHKQWQGSDLEIKAGGLYRYSSRLNVQDDYNLVPPATGPGGIALSKPLWGTIY
                     DAQWDVFNPTGTEYNPNSYNAKETIYAGYGAVRYKTALWEAGGGLRLEHTDDSWNVLQ
                     YVPGQLINGEQIYQDFLPSAYLKYQLAARQLLHFSYFRSIARPNYYELVPTGGSRATT
                     SGYITEGNYMLNHSLADNLDVRYELFPKGEEHLFIGAFYKHIRDPIELRLDTAGSSAG
                     KYAVKPLNSYPATNIGAEASFTQYWGRFGVTGNYTYTHSTISSDQKTPQGKTVQPTRP
                     MQGQTDHIVNLSLLYKDTKHGSFVQVSYQYQGTTLAATGDYAGADYYQRPMNTLAVSG
                     EKDISKRFTLFAKLNNLLNTPVQQYVQDVLVLKNTYRATYTIGIRYAN"
BASE COUNT          729 a          877 c          730 g          639 t
ORIGIN      
        1 gtcttatgtt tatctctcgg tttccgcatg gttgaatgaa acgaataacc gcacatttgc
       61 agcgcattca actcgctgtt atgcgcacaa aacaatactg cctcacttgt ttctttttgc
      121 tttttctgct ccaggccttc acccaaaccg cgacaataaa aggaaatgtt ttcgacgccg
      181 tcacccgtga cggcctcccc ggttgcaata tcgcgatcgc caataccgct tatcaccagg
      241 tcgctgacaa taagggtaat ttccagcttc tccgcctccc ggcaggagaa tatgatctta
      301 cggttaccaa tgtcggttac gtccagctcc atcgtcatat caccctcagg gaagggcaga
      361 ccctgcaatt atttctgggc ctggatctgg aaggtaaggc attggaagag gtgaccgtct
      421 tcggccgggc agatcgggaa aaggagacag gctcccgcga tcgggaaagg actgccatca
      481 atatgatgaa tgtcatctct tcccaggcca tggtacgctc tccggacatc aatgcagcca
      541 acgtgttgca gcgcatgagc ggcgtgacca tccagcgcag caacggcggc gacgaggcct
      601 atgccgtcat ccgcggtatg gagccccgct atagcaatac cctgctcaac gggataaaga
      661 tcgccagccc ggacaataag aaccggtacg tacagctcgg cattgtgccg tcggatatcc
      721 tcagcagcat cgagatcagc aagtcgctga cggccgatat ggaaggcgat gccaccggcg
      781 gaacggtgaa catggtcgtc agggacgctc ccgacaacac ctccttcaag gctacggcca
      841 gcgtcggtta cagccagctg ttcttcgatg agaaatatgt gggttttcac accggaggta
      901 tccaggataa gagtcccgtg cagcgtaacc ccccggggta tacggcccgg cccggcgatt
      961 tttcgcgcag caacctggat ttctggtcgc gacaggcatt gccgaccacc actgccggct
     1021 tctcctttac tcatcgctac ctccacgata agctgggttt cgtgctgtcg gaaaacttcc
     1081 agaaccagta ctacggcaac atcggtttcc aggccacggc cctacccttg aacgatacga
     1141 cgggccatct gcatcccaat gacgccaaca acttcaagga atatacccaa cagctgaaca
     1201 acggcctggt ggcccatctt gactatgttc taaatgataa taacaagatc agcatagaca
     1261 atttttacct ctatagctat cttgcccagg cccggatcga tggggaccac cttcttatcg
     1321 gaacaggtgg tatcggcggc aacaccggcc aggtattcaa caattatacc tcgcagaccc
     1381 agcacctgta tgtggagaac ctaaaactct cgggccagca tctcgtttcg ccggtattcc
     1441 ggctggactg ggccggtatt tactctacgg cgggtaaccg gcagcccgat gtagcgaata
     1501 tcagcaccgt ttaccttatc cagaacaatg gtgtgcaaac cggcactttt tttgacggca
     1561 tcagccgaag ctggcagaac aatgatgata aggagtactc gggtatcctc aacctggtct
     1621 accataagca atggcagggt agcgatctgg agatcaaggc cggcggtttg tatcgctatt
     1681 cctccaggct gaatgtgcag gacgattata acctcgttcc accggccacg ggtccgggcg
     1741 gcatcgcctt gagcaagcca ctctggggca ccatttacga cgctcaatgg gatgtcttca
     1801 atcctacggg cacggaatat aatcccaata gttacaatgc gaaggagacg atctatgccg
     1861 gctacggggc tgtccggtat aagacggccc tctgggaggc aggcggtggg ttgcgcctcg
     1921 agcacaccga tgattcctgg aacgtgctgc aatacgtccc cggacaactt atcaacggcg
     1981 aacagatcta ccaggacttc ctcccgagcg cctaccttaa ataccagttg gccgccaggc
     2041 agcttctgca cttctcttat tttcgcagca tcgcccggcc caattattat gaattagttc
     2101 ctacaggtgg tagcagagcg acgacatcgg gttacatcac ggagggcaat tatatgctga
     2161 atcactctct cgccgacaac ctggacgttc ggtatgaact gttccccaag ggcgaggaac
     2221 acctcttcat cggcgcattc tacaagcata tccgggatcc aatcgagctt cgcctcgaca
     2281 cagcgggatc ctcggccggc aagtacgccg ttaagccgct caacagctat cctgctacca
     2341 acatcggcgc cgaagcctcc ttcacacagt actggggaag gtttggagtg acggggaact
     2401 atacctatac gcattccacc atctccagcg accaaaaaac cccccagggt aaaacggtac
     2461 agccgacccg gcccatgcag ggacagacgg atcacatcgt caatctctct ctgctgtaca
     2521 aggacaccaa acacggatcc ttcgtccagg tatcctacca ataccagggc acgacgctgg
     2581 cagccaccgg agattatgca ggtgcggact actatcaacg tcccatgaat acgctggccg
     2641 tgtccggtga aaaggatatc agcaaacgct ttacgctgtt tgccaaattg aacaacctcc
     2701 tgaacacacc cgtacaacaa tacgtccagg acgtcctggt gctgaaaaat acataccggg
     2761 ctacttatac gataggcatt cgctatgcca attagcaaaa cccacttttt tactaaaaaa
     2821 caatacgtca tgaagatcaa caccatccac cagttcgcag ggttccgcgt tgcgagcgtt
     2881 atcgtctccg ccggtctcct gttcgccgct tgcagcaaga gctccaattc ggccaacacg
     2941 ggtgtcacat tgcctgccaa tgccatccgc agcac
//
LOCUS       DATGFG010000826         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1640280, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000826 DATGFG010000000
VERSION     DATGFG010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640280"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VKQ52_15590"
     CDS             complement(<1..1009)
                     /locus_tag="VKQ52_15590"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HLZ88678.1"
                     /translation="MLRTGLILWLSALTITLFAQQGTESDPYLLEPTPTTVAWGHYWS
                     EYPPVLKIRSGDFVKIHTLLTSNPERLEAAGVPADQVEKQLRDVQAMKDKGPGGHVLT
                     GPIYIEGADSGDILEVRLRSIDLAIPYGYNAIGAAGFLSDEIFDRKMRIIPLDAKKMI
                     GHWSDGIDIPLHPFFGSLGVAPPKEAGRWNSAPPWIHGGNLDNKELVAGTTIYYPVFV
                     KGALFEIGDGHAAQGNGEVDITALETSLHGSLQFVVRKDLHLRWPRAETPTHFIAMGM
                     DKDLTEAMKIAVREAIEFLTSEKKLSRDDAYMLCSVAVDFNVTQVVDGTKGVHAMIPK
                     GV"
     gene            1056..1259
                     /locus_tag="VKQ52_15595"
     CDS             1056..1259
                     /locus_tag="VKQ52_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88679.1"
                     /translation="MGYLLFACGVMLIALASYFISAAVYRRMVRSGSKAALGVSIVTF
                     CISALVIGFTVLVLILSNVRLER"
     gene            complement(1213..1896)
                     /locus_tag="VKQ52_15600"
     CDS             complement(1213..1896)
                     /locus_tag="VKQ52_15600"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HLZ88680.1"
                     /translation="MRTGLYILRSFFIYFILCIVTFLMLRIIIDYSAFRDNTRFLQYK
                     QDYIHDPVWRTAFYIHVFTAILALLAGFTQFSEDFLKKHRSLHRLIGRGYALIILFIN
                     FPAGMILAVNANGLLPSRIAFTLLDCCWFWFTAKAVLAARQRRIKEHKHYMIRSYALT
                     FSAITLRTWKIILSHSIHPDPLTLYMIDAWMGFVPNLLFAEWLIRRKPRSNTAIAPAA
                     HWKGSGQAR"
     gene            1948..2559
                     /locus_tag="VKQ52_15605"
     CDS             1948..2559
                     /locus_tag="VKQ52_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88681.1"
                     /translation="MQVIEKTLGLLAGIIIGSAQVIYLINTVTRKVRPSVLSWIGWAF
                     LMGTSVVAQVLSKGWQWSMTGILCSTAGCLAIGAAALLTGNYALSRKDWQYVAWGVLC
                     VGIYLLSNNPWMTTGFAIGADAVLGVPTVLKAWRDPVSERSLAWVLGVVSSSLALVIC
                     VGHDWIYVLFPAYLFLFNGMMAYLTRGRRAIAGKGPVGDGNLI"
     gene            2685..3581
                     /locus_tag="VKQ52_15610"
     CDS             2685..3581
                     /locus_tag="VKQ52_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLZ88682.1"
                     /translation="MRCNNCGWTNAEDVTHCVKCNNPLSGGSPVRASRQPTVAGRQEA
                     EGLDATLRGAAAAARDDARGGEGVARGDRVIPGEGAGRGDEADRGYAGRPDDGGGRGD
                     VAKELIACPVCQYPASSRVDSCPMCGSPLKVSGAAARPGNVAEPVAAHPGNRVEPVAA
                     RGGVVPVRDLTVDPFRQPQQGVLAPVMFLERVGRPEEPSVVLSFTAADNQVNLNRQAV
                     DENNLTITSRQQASFIFKDDKWWLVDRSDLKTTFIQVKEPVEVRDGDIVLMGDRRFVF
                     SSTPPGGEGPSGRDPGSGEEKM"
     gene            3594..4958
                     /locus_tag="VKQ52_15615"
     CDS             3594..4958
                     /locus_tag="VKQ52_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HLZ88683.1"
                     /translation="MNEFGDYESPRRFLPEIGSTIDKYLITGFLGEGTFGAVYRVERA
                     GGGVFALKLLKLWEIAYVKERQIIVKRFQREFEITCIDSPYLVRGIDFGKVEGNPYIV
                     MEFCDGGSLASYIGRVQELPNLNQVAIDVLNGMDELHRRGFFHRDIKPQNILLESGGN
                     ARVTDFGIAGHKNSTLSVRNIFGKVEQIFGTWAYIAPEQANNKVAFKALDAVSDIFSF
                     GVTMFELFTGEFPFPPYKIEDEKQLVEYLGNARSGNYRGLTVHQQRIPGQWRNIIRQC
                     LEPDYENKRFQTVKEILHCLGYQPGETAAAAYHFDKDDLCVRITYGEEMNRLYNLTQL
                     HLLLNRDAVNGRSRGRHVLTVGRKDPQVDNHVEIREDLTAYISRRHATFEVWPDQKTW
                     VVRDGQWMAGDHVWAKSMNGLYLNGKPVGMEGQAIKADDILTLGDTVLKVVASRRIPV
                     PAGR"
BASE COUNT         1051 a         1198 c         1888 g         1203 t
ORIGIN      
        1 acaccccctt cggaatcatc gcatgcaccc ccttcgtccc atccaccacc tgcgtgacgt
       61 tgaaatcgac cgccacgcta caaagcatgt aagcatcatc ccggcttaac ttcttttcgc
      121 tcgtcaagaa ctcgatcgcc tcgcgcacgg cgatcttcat cgcctcggtg aggtccttgt
      181 ccatccccat ggcgatgaaa tgggtgggag tctcggcgcg gggccagcgg aggtgcaggt
      241 ctttccgcac gacgaactgg agcgagccgt gcagtgaggt ttcgagcgcg gtgatgtcga
      301 cttcgccgtt gccctgggcg gcgtggccgt cgccgatctc gaagagggcg ccttttacga
      361 atacggggta gtagatggtt gtgccggcga cgagctcctt gttgtcgagg ttaccgccgt
      421 ggatccacgg tggggcgctg ttccagcgac cggcctcttt tggcggggcg acgccgaggc
      481 ttccgaagaa ggggtggagg gggatatcga tgccgtcgga ccagtggccg atcatttttt
      541 ttgcgtcgag ggggatgatg cgcattttgc gatcgaagat ctcatcggag aggaagccgg
      601 ccgcgccgat ggcgttgtag ccatagggaa tggcgaggtc gatggagcgc agtcttactt
      661 cgaggatgtc gccggagtct gcgccttcga tatagatggg accggtgagg acgtggccac
      721 cggggccttt gtccttcatg gcttgaacgt cgcgtagttg tttttccacc tggtcggcgg
      781 gtacgcctgc cgcttccagg cgttcggggt tggaggtgag gagggtatgg atctttacga
      841 aatcgccgga cctgatcttc agaacgggcg gatattcgga ccagtagtgg ccccaggcta
      901 cggtggtggg ggtgggttcc aggagatagg gatccgattc ggtgccctgc tgggcgaaaa
      961 gggtgatggt aagtgcggag agccagagaa taaggccggt tcttagcatg gttgtcgttt
     1021 tggtagatgt aaatatattg ttttattttt agcttatggg atacctccta tttgcctgtg
     1081 gcgtcatgct tatcgcgctg gcgtcttatt ttatctccgc cgccgtttac cggcgcatgg
     1141 tgaggagcgg tagcaaggct gcgctgggtg tcagtatcgt gaccttttgt atcagtgccc
     1201 ttgtcatcgg atttaccgtg cttgtcctga tcctttccaa tgtgcggctg gagcgatagc
     1261 cgtgtttgac cggggcttgc gccggatgag ccattcggca aagagcaggt tggggacaaa
     1321 gcccatccag gcatcgatca tgtatagggt gagcgggtcg gggtggatcg aatgcgagag
     1381 gatgatcttc caggtccgca gggtgatcgc cgaaaaggtg agggcataac tcctgatcat
     1441 atagtgtttg tgttctttta tccgccgctg cctggcggcc aggaccgctt ttgctgtgaa
     1501 ccaaaaccag cagcagtcca ggagggtaaa ggcgatccgg gaggggagga ggccgttggc
     1561 gttgaccgcg aggatcatgc ccgcggggaa gttgatgaag aggatgatga gcgcataacc
     1621 gcggccgatg agccggtgca gggaccggtg ttttttgagg aagtcttcgg agaactgggt
     1681 gaagccggcc agcagggcga ggatggcggt gaagacgtgg atatagaagg ccgttctcca
     1741 gacggggtca tggatatagt cctgtttgta ttggaggaag cgggtattgt cccggaaggc
     1801 ggagtagtcg atgatgatcc tgagcatgag gaaggtgacg atgcagagga tgaagtagat
     1861 gaagaagctg cgcaggatat agaggccggt tctcattacc ggtaagatag caaataatga
     1921 agtatatttc gccctaaaat cagttagatg caggtgatag aaaagacatt gggtttactg
     1981 gcggggatca ttatcgggag tgcgcaggtg atctatctta tcaatacggt tacgcggaag
     2041 gtgcggccca gtgtcctttc gtggatcggg tgggcttttt tgatggggac gagcgtggtg
     2101 gcgcaggtcc tgagcaaggg gtggcaatgg agtatgacgg ggatactttg ttctacggcg
     2161 gggtgtcttg cgatcggcgc ggcggcgttg ctgaccggga attatgcgct ttcgcggaag
     2221 gactggcagt atgttgcctg gggtgtgctt tgtgtgggca tttatctttt gtcaaataat
     2281 ccctggatga ccacgggttt tgcgatcggc gccgatgcgg tcctcggggt gccgacggtg
     2341 ttgaaggcct ggcgtgatcc cgtgtcggag cggtcgctgg cctgggtgct gggtgtggtg
     2401 tcgtcgagtc ttgcgctggt gatctgcgtg gggcatgatt ggatctatgt cctttttccg
     2461 gcgtatttgt ttttgttcaa cgggatgatg gcgtatctca cgcgtgggcg gagagctata
     2521 gcgggaaaag ggcccgttgg tgacggaaat ttaatttgat cttacccaat atttgcctac
     2581 tttagagata acagtaaaca cctacacgga ggaatcaata tcccatccag acagaccccg
     2641 tattcttttt tcgtacatat tcctctttga tcaaaagtat accgatgcga tgcaacaatt
     2701 gcggatggac caacgcagaa gatgtcaccc attgcgtaaa gtgtaataat cctttatcag
     2761 gcgggagccc ggtgcgtgct tcgcggcagc cgacggtggc gggcaggcag gaggcggaag
     2821 ggctggatgc gacgctgcgg ggggcggcgg ccgctgcgcg ggatgatgcc cgcgggggtg
     2881 agggtgtggc tcgtggtgat cgtgttattc cgggtgaggg tgcgggtcgt ggggatgaag
     2941 ctgaccgggg atatgctggt cggccggacg atggcggtgg ccggggggat gtggcgaagg
     3001 agttgatcgc ctgcccggtg tgtcagtatc cggcgagcag ccgggtcgac agctgcccga
     3061 tgtgcgggag tccgttgaag gtgagtgggg cggcggcgcg tccgggaaat gtggcggagc
     3121 cggtggcggc acatcccgga aatagggtgg agccggtggc ggcgcggggt ggggtagtgc
     3181 cggtgcgtga tctgacggta gacccgttcc ggcagccgca gcagggggtg ttggcgccgg
     3241 tgatgttcct ggagcgggtg ggccggcccg aggaaccctc ggtggttctg tcttttacgg
     3301 ctgcggataa ccaggtgaat ttgaaccgcc aggccgtgga cgagaacaat cttacgatca
     3361 cgagccggca gcaggcttcc tttattttta aagatgacaa gtggtggctg gtagacagaa
     3421 gcgacctgaa gacgacattt atccaggtga aggagcccgt ggaagtcagg gatggggata
     3481 ttgtgctgat gggcgaccgg agatttgtgt tttcttctac ccctcccggc ggcgagggtc
     3541 ccagtggcag ggatcccggc agtggagagg aaaaaatgta aacccgtttt acgatgaacg
     3601 aattcggtga ttatgagagt ccccgacgtt tcctgcccga gataggttcc acaatagaca
     3661 aatatctgat caccggtttt ttgggggaag ggacgtttgg ggcggtgtac cgggtggagc
     3721 gtgccggggg cggggtcttt gccctgaagc tattgaagct atgggagatc gcttatgtca
     3781 aggagcggca gatcatcgtc aagcggtttc agcgggagtt cgagatcacc tgtatcgatt
     3841 cgccgtacct ggtgcggggg atcgactttg ggaaggtgga ggggaacccg tatatcgtga
     3901 tggagttttg cgatgggggg agcctggcca gctatatcgg cagggttcag gaattgccga
     3961 atctcaacca ggtggcgata gatgtgctca acgggatgga tgaactgcac cgccggggtt
     4021 ttttccaccg cgatatcaag ccgcagaata tcctgctcga atcagggggg aatgcgcggg
     4081 tgaccgattt cggcatcgcg gggcataaga attccacgct ttcggtgcgg aatatttttg
     4141 ggaaggtgga gcagatcttt gggacctggg cctatatcgc cccggagcag gccaacaata
     4201 aggtggcctt taaggccctc gatgccgttt cggatatctt ttcctttggc gtgacgatgt
     4261 tcgagctatt tacgggggag ttcccgttcc caccctataa gatcgaagat gagaagcaac
     4321 tggtcgaata cctgggtaac gcgcggagcg gcaattaccg ggggctgacg gttcatcagc
     4381 agcggatacc cgggcagtgg cgaaatatca tccggcagtg tctcgagccg gattatgaga
     4441 acaagcgatt ccagaccgta aaggagatac tgcattgcct gggttaccag cccggggaga
     4501 cggccgccgc ggcctatcat ttcgataagg acgatctctg tgtccggatc acctatggag
     4561 aggagatgaa ccggctctat aatcttaccc aactgcattt gttgttaaac cgggacgctg
     4621 tgaacggtcg ctcgcgtggg cgtcatgtac ttacggtggg ccggaaggat ccccaggtgg
     4681 acaatcatgt cgagataagg gaggatctga cggcgtatat ctcgcgccgg catgcgacgt
     4741 tcgaggtgtg gccggatcag aagacctggg ttgtgcggga cgggcagtgg atggcggggg
     4801 atcatgtctg ggcgaagagt atgaacgggc tttaccttaa tgggaagcct gtggggatgg
     4861 aggggcaggc gataaaggcc gatgatatcc ttacgctggg ggatacggtg ctgaaggtgg
     4921 tggcgagccg gaggattccg gtgccggcgg ggcggtgaag ttgcggccgg gtagtgcgcg
     4981 gaggcggagg atgtggccgg ggaaagacgt ggacggggag gatgtggtct gggcaggacg
     5041 tgggccggga gtccggggcc ggggcaggac gcgggccggg tggtcagtga ctggttagcg
     5101 accgggtgtt ataccgcaaa ccgggttcga ttgcatttat tttggaacat cataaatcaa
     5161 tgatccatgg caaatcccac agtaaacccc gaccggccca ccgataagaa gtttgcgaag
     5221 agttttggcg gaagcctccg gtcgctgacg ggtggcaagg gccgcaaata ttttgtgctg
     5281 gagcagctga cggcttccga caagcaccgg gcgggggaga gtacggaatt catcggcgac
//
LOCUS       DATGFG010000827         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_568864, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000827 DATGFG010000000
VERSION     DATGFG010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568864"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /gene="bioB"
                     /locus_tag="VKQ52_15620"
     CDS             <1..955
                     /gene="bioB"
                     /locus_tag="VKQ52_15620"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HLZ88684.1"
                     /translation="VRALYDRPLFELLDEARSVHRGHHVDSEVQLCTLLSVKTGGCPE
                     DCAYCPQAARYSTGVNVQALMKKEEVLEYAAKAKAAGSTRFCMGAAWREVRDNKDFDR
                     VLDMVKGVNEMGMEVCCTLGMLNEDQAKRLAEAGLHAYNHNLDTSAEYYGEIISTRTY
                     DDRLHTLDNVRKAGVTVCCGGIIGLGESHDDRIGMLYTLSNLAKHPESVPINALVRVK
                     VTPLENNAKVDTWDMIRMIATARILMPSTMVRLSAGRAEMTQAEQALCFMAGANSIFA
                     GEKLLTTPNPSFEEDNLMFGLLGLRPRVAFKDEKEMAAELH"
     gene            complement(1002..1421)
                     /locus_tag="VKQ52_15625"
     CDS             complement(1002..1421)
                     /locus_tag="VKQ52_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88685.1"
                     /translation="MINLIIVDDHFHVREAWSWVLNQVPGFNVIAQCANGQEAIEAAQ
                     RLQPDVMLMDINMNPVNGIEATRTIRKFAPDIKIIGVSVQAERSYVNEMLRNGANGYV
                     TKNSSSQEMVTAIDEVLAGKTYICEEVGHLRPAVAAR"
     gene            complement(1663..>2798)
                     /locus_tag="VKQ52_15630"
     CDS             complement(1663..>2798)
                     /locus_tag="VKQ52_15630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HLZ88686.1"
                     /translation="KTYIGAMPGRILQSLRKIKTSNPVMILDEIDKTGSDFRGDPSSA
                     LLEVLDPEQNHTFYDNYLELEYDLSKVLFVATANDINAIQPALRDRLEIIDLSGYAVE
                     EKVEIARRHLVPKQKDAHGLKPINFKIGDKVLEKVIQDYTRESGVRELDRQLASIMRY
                     QAKEYAMKGKLKAQLTQADIEKMLGKSKYSNDLYKVANMPGVAVGLAWTYVGGDILFV
                     ETSLSDGKGDLKLTGNLGNVMKESAVTAFTYLQSNARKYDIDPEAFNKKTIHVHVPEG
                     AVPKDGPSAGVTMMSAMASAFTGKRVKPYLAMTGEITLRGQVLPVGGIKEKILAARRA
                     GLKEVILCWQNEKDVLEIDSSFIRGLKFNYVKTMQQVLELALV"
BASE COUNT          597 a          822 c          763 g          616 t
ORIGIN      
        1 cgtgcgcgca ctttacgatc gcccgctgtt cgagctgctc gacgaggcgc gctccgtgca
       61 ccgaggtcac cacgtcgaca gcgaagttca gctctgcact ctcctcagcg tgaagaccgg
      121 gggctgcccc gaggactgcg cctactgtcc gcaggcggcc cgctatagca cgggggtcaa
      181 tgtgcaggcg ctgatgaaga aagaggaagt gctggaatac gccgcaaagg caaaagccgc
      241 cggctcgacc cggttctgca tgggcgccgc ctggcgggaa gtgcgggaca acaaggattt
      301 cgaccgggtg ctcgatatgg taaaaggggt gaacgaaatg gggatggagg tctgctgcac
      361 cctcggcatg ttgaacgagg accaggccaa acgcctggcc gaagccgggc tccacgccta
      421 caaccacaat ctcgatacct ctgccgaata ctacggcgag atcatttcca cccgtacgta
      481 cgacgatcgg ctgcataccc tggacaatgt ccgcaaggcc ggggtgaccg tctgctgcgg
      541 cgggatcatc ggtctcggcg aatcccatga cgaccgcatc gggatgctgt ataccctttc
      601 caaccttgcc aaacatccgg aaagcgtccc tatcaacgcc cttgtacgcg taaaggtcac
      661 cccgctggag aacaatgcaa aagtcgatac ctgggatatg atccggatga tcgccacggc
      721 gcggatcctc atgccgtcga ccatggtaag gctgagcgcc ggccgggccg aaatgaccca
      781 ggcggaacag gccctctgtt tcatggccgg ggcgaacagt atcttcgctg gtgagaagct
      841 gctcaccaca cccaatccct ccttcgaaga ggacaatctt atgttcgggc tcctgggact
      901 cagaccgcgg gtcgcgttca aggatgagaa ggaaatggca gcggagctgc actaaacagg
      961 tgctggcggg cggcgcgcta atggggggcg agggccggat gctagcgggc ggcgacggcg
     1021 ggccgcagat gacctacctc ctcgcagata tacgtcttgc ctgccaacac ctcgtcgatg
     1081 gcggtcacca tctcctggct ggaggaattc ttggtgacat acccgttggc tccgttccgc
     1141 aacatttcgt tgacatacga acgttccgcc tggacggaaa cgccgatgat ctttatgtcg
     1201 ggggcgaact tccggatcgt gcgggtcgct tcgatcccgt tgacggggtt catattgata
     1261 tccatcagca tcacatcagg ctgtaatcgc tgggcggcct cgatggcctc ctgcccattg
     1321 gcgcactggg ctatcacgtt gaatccggga acctggttga gcacccagct ccaggcttcg
     1381 cggacatgga agtgatcgtc gacgataatt aagttgatca tggaaggttt tttctcacgc
     1441 tcccaaaatt actgtttcgc gaataaaaaa aaagtaaatc ggttaaatca gcaaacgatt
     1501 acccattttc ctttaagcaa taaaccttac aaaaagggcc cggcgcatgc gccgggcccc
     1561 taacccttaa aacaaccaac atgaaaatct gtaatattaa cagctcatgc gttgtcaagg
     1621 ttcacaacaa tatcataaat cggacggcgt ccggcccggt aatcacacca gggccagctc
     1681 caaaacctgc tgcatcgtct tgacataatt gaatttgagc ccccggataa aggaggaatc
     1741 gatctccagc acatccttct cattctgcca gcagaggatc acctctttca gccctgccct
     1801 tctggccgca agtatctttt ccttgatacc gccgacgggt aacacctgcc cgcgcagcgt
     1861 gatctcgccc gtcatggcca aatacggttt gacccgcttg ccggtgaagg cggaagccat
     1921 cgcggacatc atcgtcacac ccgcgctggg cccgtctttg ggcacggcgc cctccggtac
     1981 gtgcacatgg atggtcttct tgttaaaggc ctccggatcg atatcgtatt tgcgcgcgtt
     2041 ggattgcaga taggtgaacg cggtgacggc gctctctttc atgacattcc ccagattgcc
     2101 ggtgagcttc aggtcgccct tgccgtcgct taggctggtc tccacgaaca ggatatcacc
     2161 gcctacatag gtccaggcca atcctacggc cacaccgggc atattggcca ccttatagag
     2221 atcgttgcta tacttcgatt tccccagcat cttctcaata tcagcctggg tcagctgcgc
     2281 tttgagcttg cccttcatgg catattcctt cgcctggtag cgcatgatcg acgccagttg
     2341 ccggtcgagc tcccggacac cgctttcgcg ggtgtaatcc tggatcactt tctcgagaac
     2401 cttgtcgccg atcttgaaat tgatcggttt cagcccatgg gcgtcctttt gtttgggaac
     2461 gaggtgacgc cgggcgatct ctaccttctc ctccaccgcg tacccgctca aatcaatgat
     2521 ttccaatcgg tcgcgaagcg ccggctggat ggcgttgata tcgttggccg tagcgacgaa
     2581 gagcaccttg ctgagatcgt attccagttc caggtaattg tcatagaagg tatggttctg
     2641 ttcggggtcc agcacttcca gcagagcgga gctgggatcc cccctgaaat cgcttccggt
     2701 cttatcgatc tcatccaata tcatcaccgg attcgaagtt tttattttcc ggagggattg
     2761 taatatccgt cccggcatgg ccccgatata ggtctttc
//
LOCUS       DATGFG010000828         5310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_620799, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000828 DATGFG010000000
VERSION     DATGFG010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620799"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3055
                     /locus_tag="VKQ52_15635"
     CDS             <1..3055
                     /locus_tag="VKQ52_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ88687.1"
                     /translation="YTRLSYELLPDISQPTLVITTNYPGAAPANVEQTVTKKIEDQLS
                     SIDAIKTITSQSLEGVSIITAEFNTGSNIDLKQQEVQRKINNIKGDMPTDVKSSGISR
                     VSPSDQPIMQLMAVSNLDNAAFYDIVKNQINPLLQQVPGVGEITLIGGQEREIQVNIN
                     KEKLDYYGLSILNVTQAVANGNQEFPAGKVKSETLGQMTVRLSGKYSSVEQLNNLVVG
                     LPVAGSPIRLSDVATVADVTKDVSTVFRYNGQNGIGMLIKKQGDANAVAISKQVKERI
                     TTLEKRYAAEGVKFTIADDTADYTLEAANGVVDDLMLAVILVAVIMLLFLHSIRDSLI
                     VLVAIPASLISTFIALYLFGYTLNLMTLLGLSLVIGILVDDSIVVLENIHRHLAMGKD
                     KRTAALDGRKEIGFSAVAITMVDVVVFGPLCLIQNSIGSILRAYSSTIVVATLLSLFV
                     CYTLTPWLASRFGKITILDNKKILERPLIVFEKLIGGLTHWYSQALAWVLRHKTIMAG
                     IVVLLFVMTAAIMKLGVIGNEFVTQSDKGQFRLALEYDKNSTVLQNNLRTREIEDYLR
                     QQPDITTVFSNVAGASTSSIVAGVGSDYKSELTVTLVDKKDRRLPTEKLMLEKSRDLL
                     QHFPGVKISPTMVSMINSGEPIQIVLNGENTTQLMAAARDLKRRIQAIPGAINVSLSV
                     EDGNPEVEVNILRERMGQLGLTINQVGAVLQNAYAGNDDAKYRVGTNEYDIKVKFDDF
                     DKHSVEDVKNIAFINATGQLIKLSQFADVVQGSGPSVLERKDRRTSVTIKSDVLGITS
                     GVVADEIKRSLSTQPLPAGIDLRWSGDIERQGDSFGAMAMAIIAAILLMYLVMVALYN
                     NFVYPFVVLFSIPVAFIGVFLALALTRSSMSIFTILGMIMLLGLVAKNAILIVDFANH
                     RKEHGDSTWQALLEAGKERLRPILMTTLAMVIGMLPIALSTAAGSEWKSGLAWVIIGG
                     LSSSLCLTVFVVPMVYYCVDRIGDKFRRWLSARNKRESAAITARL"
     gene            complement(3125..3874)
                     /locus_tag="VKQ52_15640"
     CDS             complement(3125..3874)
                     /locus_tag="VKQ52_15640"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HLZ88688.1"
                     /translation="MKQLLFIPGFCMLILLSLTRCGKQTIVHSTDTLIVKIHDTTAIH
                     DTATRTLTVKPGPNDGQDCLVSNMPLYAAANLSGNPDITASEWTYYAQGYGEATTRTY
                     IEFVALDGLPSNASIKSAKLSFYGLSSGFALPTGNSDYPGSPYGSLGNSCWLKRVLGN
                     WNEDSITWNNMPPTTDSNRVSVPASTSQWNYNTVVDVTPLVQDIVNTGRNYGFCLQLQ
                     TEQIYRGLNFAGSRNADSTRWPKLEVTYSVH"
     gene            4035..4781
                     /locus_tag="VKQ52_15645"
     CDS             4035..4781
                     /locus_tag="VKQ52_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ88689.1"
                     /translation="MIRTILVDDEIDSILILKNLLGSSCPEVEVVGEADSVETALQTI
                     KQGNPDLVLLDISLKNKDAFELLGLLGPVGFQLIFVTAWDQHAIRAFKYNALDYLLKP
                     VDGDELRKAIDRVARRMQEGTERTDWKTVLDSIASLQQSQQKMAIPTMTGLSFIHMGE
                     ILRFEAKGSCTAIIMDNGGSLVSTRSIKEYEALLPGSVFYRVHYSHIINMNKVQKYLK
                     GRGGSLIMDDGRTIDVSSRRKDEFLKRLMK"
     gene            4789..>5310
                     /locus_tag="VKQ52_15650"
     CDS             4789..>5310
                     /locus_tag="VKQ52_15650"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ88690.1"
                     /translation="MRNVFLFLVLILPFLPVFSQAQEYSYAHYGIAEGLAGATVYSLT
                     QDRDGFLWAGTETGLSRFDGTHFTNFTTKDGLPGVEVLQVFADSRGRVWMALLGRSVC
                     YYYQGRIHTGQNDSLLRRIRLTGNVNGFAEDPAGNILFPDMECLHVIRADGTLLDIDS
                     IGHLPVRDCLAVGT"
BASE COUNT         1276 a         1415 c         1492 g         1127 t
ORIGIN      
        1 ctacacccgc ctgagctacg agctcctgcc cgatatctca cagcccacac tggtcatcac
       61 gacaaactat ccgggtgcgg cgcccgccaa tgtcgagcag acagtcacaa aaaagataga
      121 ggaccagcta agctccatag atgccataaa gaccatcacc tcccaatccc tggaaggggt
      181 atcgatcatc accgccgaat tcaacacggg cagcaatatc gatctcaaac agcaggaagt
      241 acaacggaaa ataaacaata tcaagggcga catgcccacg gacgtaaaat cgtccgggat
      301 ctccagggtg tcgcccagcg accagcccat tatgcagctg atggcggtat ccaacctcga
      361 caatgccgcc ttctatgaca tcgtcaaaaa ccagataaac ccgctgctgc agcaggtgcc
      421 gggagttggg gagatcacgc tcatcggtgg gcaggagcgg gagatacagg tgaacatcaa
      481 caaggagaaa ttggattatt atggcctgtc cattttaaat gttacccagg cggtagccaa
      541 tggcaaccag gaattcccgg ctggcaaggt aaagtcggaa acactggggc aaatgacagt
      601 ccggctttca gggaaataca gttcagttga acaattgaac aacctcgtgg tcggcctgcc
      661 cgtcgccggc agccccatcc ggctcagcga cgtggccacg gtcgcggatg tcaccaaaga
      721 cgtttccacc gtcttccgct acaacgggca aaacggtatc ggtatgctga taaagaaaca
      781 gggcgatgcc aatgccgtcg ccatcagcaa acaggtaaag gaaaggatca ccacgctgga
      841 gaaaaggtat gccgcggaag gcgtaaaatt taccattgcc gacgataccg cagactatac
      901 cctggaagcc gccaacggcg tcgtggatga cctgatgctg gccgtgatcc tggtggccgt
      961 catcatgctg ttgttcctgc acagtatccg tgactcgctg atcgtgctgg tcgccatccc
     1021 cgcttcactg atctccacct ttattgccct ctacctgttt ggctataccc taaacctgat
     1081 gaccctgctg ggactgtcgc tggtgatcgg catattggtg gatgacagca tcgtggtgct
     1141 ggaaaatatc caccggcatc tggccatggg caaggataag cgcaccgcgg ccctcgacgg
     1201 gcgaaaggag ataggcttct cggctgttgc catcacgatg gtggatgtcg tggtgttcgg
     1261 ccccctgtgc ctgatccaaa atagcatcgg ctcgatcctg cgcgcttatt cctccactat
     1321 cgtcgtagcc accttattaa gcctgttcgt ctgttataca ctcaccccct ggctcgcatc
     1381 ccgtttcggt aagatcacca tcctggacaa caaaaagatc ctcgaaaggc cattgatcgt
     1441 atttgaaaaa ttgatcggcg ggctcaccca ttggtatagc caggcgcttg cctgggtact
     1501 gcgccataag acgatcatgg ccggtatcgt cgtcctgctt tttgtcatga cggcagccat
     1561 catgaaactg ggtgtcattg gcaatgagtt tgtgacccag agcgataagg gacaattcag
     1621 gttggcattg gagtacgaca agaacagcac cgtactgcag aataatttac gcacccggga
     1681 gatcgaggac tacctgcggc aacagcccga cataaccacg gttttcagta atgtggccgg
     1741 ggcgagcacc tcttccatcg tggccggtgt agggtcggat tacaaatcgg agctgacagt
     1801 caccctggtg gataaaaaag accgccggct accgaccgaa aaactgatgc tggagaaaag
     1861 ccgtgacctg ttgcagcact ttccaggggt gaagatcagt cccaccatgg tcagcatgat
     1921 caacagcggc gagcccattc aaatagtgct gaacggcgag aacaccacac aattgatggc
     1981 ggccgccaga gacctgaaac gccgcatcca ggcaataccc ggcgccatta acgtaagcct
     2041 cagcgtggaa gacggcaacc cggaggtgga agtgaatatc cttcgggaaa gaatgggcca
     2101 actggggctt accatcaacc aggtaggcgc cgtcctgcaa aacgcctatg cgggcaacga
     2161 cgatgccaag taccgcgtgg gtaccaacga atacgatata aaggtcaagt ttgacgactt
     2221 cgacaagcac agcgtggaag acgtaaagaa catcgcgttt atcaatgcga cagggcagtt
     2281 gataaagttg tcgcaattcg ccgatgtggt gcagggcagc ggccccagcg tattggaacg
     2341 aaaagaccgc cgcacctcgg tcaccataaa aagcgacgtg ttggggatca cgtccggagt
     2401 agtggcggat gagatcaaaa gatccctttc aacacaaccc ctgccggccg gcatcgatct
     2461 gcgctggagt ggcgatatcg agcgacaggg cgattctttc ggcgcgatgg ccatggccat
     2521 cattgcggcg atcctgctga tgtacctggt gatggtggcc ctatacaata atttcgtgta
     2581 cccgttcgtg gtcctgttct ctatcccggt ggcgtttatc ggcgtattcc tggcgctggc
     2641 gctgacaaga tcatccatga gcatcttcac gatcctcggt atgatcatgc tcctgggcct
     2701 ggtggccaag aatgccatcc tcatcgtcga ctttgccaat caccggaagg agcatggcga
     2761 tagcacctgg caggcactgc tcgaggccgg taaagaacga ctccgcccca tcttaatgac
     2821 tacgctggca atggtgatcg gcatgctgcc cattgccctt tccaccgcgg cgggcagtga
     2881 gtggaaaagc ggcctggcat gggtgatcat tggcgggctt tcctccagcc tctgccttac
     2941 ggtattcgtg gtgccgatgg tgtattactg cgtggatagg ataggggaca aattcaggag
     3001 atggctttca gcgaggaaca aaagagaatc ggccgcgatc acagcccggc tttgatctat
     3061 agacaggtgt tagtttaccc ccgaccagga gtaaggtcgg gggctttttt tatcgtactt
     3121 actatcaatg aaccgaatag gtgacttcca gtttcggcca acgcgtggaa tccgcattcc
     3181 ggctgccggc aaaattgaga ccccggtaga tctgctccgt ttgaagctgc aggcagaacc
     3241 cgtagtttcg gccggtgttg acgatatcct gcacaagggg ggtgacatcg acaacggtgt
     3301 tataattcca ctgcgaagtg gacgcgggaa cgctgaccct gttggaatcc gtcgtcggcg
     3361 gcatgttatt ccaggtaatg gagtcctcgt tccagttgcc caacaccctt ttcaaccagc
     3421 aggaattgcc aagggaacca tagggcgatc cggggtagtc ggaattgccc gtcggcaggg
     3481 cgaacccgct ggacaatcca tagaaagaga gttttgccga ctttatcgag gcattgctgg
     3541 gcaagccgtc cagggcgacg aattcgatat aggtgcgcgt ggtggcttcc ccatatccct
     3601 gggcatagta ggtccactcg ctggcggtga tatcagggtt tccactgaga ttggccgcgg
     3661 cgtagagggg catgttggat accaggcaat cctgcccgtc gttaggcccc ggctttaccg
     3721 taagggtcct ggtggcggtg tcatgaatgg cagtagtgtc atggatcttg acgatcaggg
     3781 tgtcggtgct atgtacgatg gtctgctttc cacacctggt caggcttaac aggatcagca
     3841 tacaaaagcc cgggataaat aggagctgtt tcatgggtca aaacaaataa aagaagcccg
     3901 gaaaaggaag ctataagtga catctgctgc atttgaggga taactgcgtc gaaaacggac
     3961 atagccgtaa aaatggatgc attcaaaaca ttttattacc ttgtggcaac accagtcaaa
     4021 cgccggctaa tcttatgatc cgcactatct tggttgatga tgagatcgac agcatcctga
     4081 tcctgaaaaa tttactgggc tcctcctgcc cggaagtaga agtcgtcggc gaggccgaca
     4141 gcgtagaaac ggcattgcag accattaagc aagggaaccc cgatctcgtc ttgctggata
     4201 tttccctgaa aaataaggac gcattcgaac tgctgggtct cctgggcccg gtcggttttc
     4261 agttgatctt cgtaacagcc tgggaccagc atgccatccg tgcattcaaa tacaacgcgc
     4321 tggactatct cctgaagccg gtggatgggg atgagctgcg taaggccatc gacagggtgg
     4381 cccgtcggat gcaggaaggg acggaaagga cggattggaa aacggtgttg gacagcattg
     4441 cctctttgca gcaatcacag cagaaaatgg ccattccgac aatgacaggc ctgtcgttca
     4501 tccacatggg cgagatcctg cgattcgagg cgaagggttc ctgtacggcg atcatcatgg
     4561 acaatggggg aagcctggta tccacaaggt cgataaagga gtatgaagcg ctgttgccgg
     4621 gctcggtctt ttaccgggtg cactattcgc acattatcaa catgaacaaa gtccagaaat
     4681 atctcaaagg cagaggcggt agcctgatca tggacgacgg ccggacgatc gacgtgtctt
     4741 cccgtcgcaa ggatgaattc ctcaaaaggc tgatgaagtg aacggcctat gaggaatgtc
     4801 ttcctgttcc tggtcctcat cctgccattc ctgccggtct tctcccaggc gcaggaatac
     4861 agctatgcac attacggcat cgcggaaggc ctggctggcg ccacggtgta tagccttacc
     4921 caggataggg atggcttcct ctgggcaggt acggaaacag gcctgagtcg tttcgacggg
     4981 acgcatttta ccaattttac cacgaaggac ggccttcccg gtgtggaagt gctccaggta
     5041 ttcgccgatt cgcgcggtag ggtgtggatg gcgctactcg ggcgatccgt ctgctattac
     5101 tatcagggcc ggatccacac cgggcagaat gactccctgc tgcgacgcat ccggttgacc
     5161 gggaatgtca atggctttgc cgaggacccg gccggcaata tcctattccc ggacatggaa
     5221 tgtttgcacg tcatccgcgc ggacgggacc ctgctcgaca tcgattccat cggccacctg
     5281 cccgtccggg actgcctggc ggtcggcacg
//
LOCUS       DATGFG010000829         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1640347, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000829 DATGFG010000000
VERSION     DATGFG010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640347"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VKQ52_15655"
     CDS             <1..1157
                     /locus_tag="VKQ52_15655"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLZ88691.1"
                     /translation="NLLIDLLGEMGVKIERPHRDTCIFQADEVNTDYLRSEAYRQKSG
                     RLRGSVMLAGPMLARYRKAFISKPGGDKIGRRRLDTHIIGFEKLGVRFEYQQDEGLFH
                     LDARNLKGCYLLLDEPSVTGTANIVMAAAMAEGTTTIYNAACEPYIQQLCRMLNRMGA
                     QISGIGSNLLTIKGVGYLGGTDHRMLPDMIEIGSFIGLAAMTQSDITIKNTSLDNLGI
                     IPDKFRQLGIQLEFQGDDIRVPEQEIYEIQNFLDGSVLTIYDHPWPGFTPDLLSIVLV
                     VATQARGSVLIHQKMFESRLFFVDKLIDMGAQIILCDPHRAAVIGMAREQRLRGITMS
                     SPDIRAGVALLIAALSAEGRSIIQNIEQIDRGYQHIDHRLRQLGAQINRI"
     gene            1161..1778
                     /gene="gmk"
                     /locus_tag="VKQ52_15660"
     CDS             1161..1778
                     /gene="gmk"
                     /locus_tag="VKQ52_15660"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLZ88692.1"
                     /translation="MSEKPISPRIIIITAPSGAGKTSITRYLLGKYPHDLAFSVSAAT
                     RNPRSYEKDGVDYYFIGMENFKQKIQHNEFVEWEMVYEGKYYGTLKSELQRIWSEGKT
                     PLLDIEVKGAIHIREQFPDSTLALFIEPPSVDELRRRLEMRGTETVESLLARVNKASY
                     EISFKHHFDRIIVNDDLNKACFEAEAAVTGFLGLKAPQTGMPPVS"
     gene            1848..3125
                     /locus_tag="VKQ52_15665"
     CDS             1848..3125
                     /locus_tag="VKQ52_15665"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HLZ88693.1"
                     /translation="MINTNTLVWIVIAWLMIAFFAGIEVAFLRANKLTIELKKKQGLS
                     SGIILANFMDNPAQFIGTALAGFTIFLVIFGLMVGETFLPLWNILITKIHMPGSYVNA
                     IRLFAETLFASIFILLFGEFIPKALFRAKSDSLLGFFAGTMDFFNRLFYPLASFFASI
                     AQWILKYVFNVRIDERKDTFSGTDLEHFLTQTGDLEEDSKDLNKSLFEAALALPKVRV
                     RECLVPRKEIEGIDIGLSMEAVRKKFVQTRLSKLVVYDGNLDHVTGYIHQLDLFKDPP
                     SIKAILHPIPAVPESMSATDLINKFSRERKSIAWVVDEFGGTAGIVTMEDLLEEIFGD
                     IRDEYDTEEFEERKLSNDEFILSGRLELDYLNRKYGFDLPENESETLSGFIINQHETI
                     PHLKDRIIIGDYEFEILDVSDTRIEMVRLKVLK"
BASE COUNT          789 a          979 c          829 g          698 t
ORIGIN      
        1 taaacctgct catcgacctg ctgggggaga tgggggtaaa gatcgagcgg ccgcatcgcg
       61 atacctgtat cttccaggcc gacgaggtga acaccgacta cctccgcagc gaagcctatc
      121 gccagaaaag cggccggctc cgcggctccg tcatgctggc cggtcccatg ctggcccgtt
      181 accgcaaggc ctttatctcc aaacccggcg gcgacaagat cggccggcgc aggctggata
      241 cccatatcat cggcttcgaa aaactggggg tccgcttcga ataccagcag gatgaaggac
      301 tttttcacct cgacgcccgg aatttaaagg gttgttacct cctgctggac gaaccctccg
      361 tcaccgggac ggccaatatc gtcatggccg ccgccatggc cgaaggcact accaccattt
      421 ataatgccgc ctgcgaaccc tatatccagc agttgtgcag gatgctgaac cggatgggcg
      481 cccagatcag cggcatcggt agtaacctgc tcactatcaa aggggtaggc tatctgggag
      541 gcaccgatca ccggatgttg ccggatatga tcgagatcgg gtcctttatc gggctcgccg
      601 ccatgaccca aagtgatatc accatcaaaa atacttccct cgacaacctc ggcatcatcc
      661 ccgacaagtt cagacagctg ggcatccagc tggaattcca gggagacgat atccgcgtcc
      721 ccgaacagga gatctatgag atccagaact ttctggacgg atcagtgctg acgatctacg
      781 accacccatg gccgggcttt accccggatc tgctcagtat cgtgctcgtc gtggctaccc
      841 aggccagggg atcggtgctc atccatcaga agatgttcga aagccgttta ttctttgtcg
      901 acaaactgat cgatatgggc gcccagatca tcctctgcga cccgcaccgc gcagccgtaa
      961 taggaatggc ccgcgaacag cgcctgcggg gtatcacgat gagcagcccc gatatccggg
     1021 ccggcgtcgc cctcctgatc gccgccctca gcgcggaggg gcggagcatc atccagaata
     1081 tcgaacagat cgaccgtggt tatcagcata tcgaccaccg gttacggcaa ctaggcgccc
     1141 aaatcaaccg gatataggct atgagcgaga aacccatctc tccaaggatc attatcataa
     1201 ccgccccctc gggtgcgggg aagacctcca ttacccgcta tctcctggga aagtaccccc
     1261 acgatctcgc cttttccgtg tcggccgcca cccgcaaccc ccgctcgtat gaaaaggatg
     1321 gggtagacta ctatttcatc ggcatggaga atttcaaaca aaagatccag cacaacgagt
     1381 tcgtggaatg ggaaatggtc tacgaaggga agtattatgg caccctgaaa agtgagctgc
     1441 aacgcatctg gagcgagggc aaaacccccc tcctggacat cgaggtcaag ggggccatcc
     1501 atatccggga acaatttccc gactccaccc tggccctttt tatcgagccg ccctccgtgg
     1561 acgaattacg ccgccgcctc gagatgcggg gcacggagac cgtggaaagc ctcctggcca
     1621 gggtaaacaa agcctcctac gagatctctt ttaaacatca tttcgaccgg atcatcgtca
     1681 atgacgacct gaataaggcc tgcttcgagg ccgaagccgc cgttacgggg tttctgggcc
     1741 ttaaagcgcc gcagacagga atgccccccg tctcctgacc tatccctgga aaatatcgaa
     1801 ttttccgagt attttcgtca cccggacagg aacccaaatt gttgattatg atcaatacga
     1861 atacccttgt gtggattgtt attgcgtggc tgatgatcgc cttttttgca ggcatcgagg
     1921 tcgcgttcct gcgggccaac aaactgacca tcgaactgaa gaaaaagcag gggctcagca
     1981 gcgggatcat cctcgccaat tttatggaca acccggccca gttcatcggc accgccctgg
     2041 ccggattcac cattttcctc gtcatcttcg gtctaatggt gggcgagacc ttcctgcccc
     2101 tctggaatat cctgatcacg aagatacaca tgcccggtag ctatgtcaac gctatccggc
     2161 tttttgccga aaccctcttt gcctccatct tcatcctgct cttcggtgaa ttcatcccaa
     2221 aggccctgtt ccgcgccaaa agcgattccc tgctgggttt tttcgcggga acgatggact
     2281 tcttcaaccg gctcttctat ccactcgcca gcttctttgc gtcgatagcg cagtggatac
     2341 tgaaatatgt gttcaacgtc cgcatcgacg aacgaaagga caccttctcg ggcaccgacc
     2401 tggaacattt cctgacgcag acgggcgacc tggaagaaga cagcaaggat ctgaataaaa
     2461 gtcttttcga agccgccctg gcgctgccca aggtcagggt ccgcgagtgc ctcgtccccc
     2521 gcaaagagat cgaaggcatc gacatcgggc tgagcatgga ggccgtgaga aagaagtttg
     2581 tccagacaag actgagcaaa ctggtcgtct atgacggtaa cctggaccat gtcacgggct
     2641 atatccacca gttggacctc tttaaggacc caccctccat caaggcgatc ctccatccca
     2701 tccccgcggt acccgaaagt atgagcgcca ccgatctgat caataaattc agccgcgaac
     2761 ggaaaagcat tgcctgggtc gtcgatgaat tcggcggcac cgccggcatc gtgacgatgg
     2821 aagacctgct cgaagagatc tttggcgaca tccgcgatga atacgatacc gaagagttcg
     2881 aggaaagaaa attatccaat gacgaattca tcctctccgg ccggctcgag ctggattatc
     2941 ttaaccggaa gtatggcttc gacctgcccg aaaatgaatc ggaaacgcta tccggtttca
     3001 tcatcaacca gcacgagacc attccccatc taaaggaccg catcatcatc ggcgactacg
     3061 aattcgagat cctggatgtc agcgacaccc ggatcgaaat ggtacgcctg aaagtgctga
     3121 agtaggccac gaacctgtcg ttctttggct cccggacccc ttgttaacca atgttaaccc
     3181 gtggttaaac agcctccatt atttttccat tctgcacttt ttgagcaatt tcgtcctcgt
     3241 aaagcagcaa aatccaactt tttcataggt cagttaggag taaacaatgc actct
//
LOCUS       DATGFG010000830         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_631522, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000830 DATGFG010000000
VERSION     DATGFG010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631522"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="VKQ52_15670"
     CDS             complement(<1..1604)
                     /locus_tag="VKQ52_15670"
                     /inference="COORDINATES: protein motif:HMM:NF039454.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6443 domain-containing protein"
                     /protein_id="HLZ88694.1"
                     /translation="MTVQQMTAVKKMSRLLPPLTFRLALLFCIGVCLSPTVRSQQYPA
                     KTGPVYDEAINYLSSTHGGGLTTGATIPLTYTSAGEKTPLKITNIVSLKLNEEATTYI
                     PDNFQASVTVQVDYGASPSATTSVTQILTISYSKDGGVSYNPKQYLSFTGAQYVQVTI
                     TNVPANSLSNGVSVISLLSVCNEMHIWRDYILSPSGSVTPTLQVNTPATTGVPDELPV
                     QWSLPAGYTGHNGLQLEWAWLETELASLYTQNGSINYDLLFRNNSTRVSLPLGTTGYN
                     IPLYYDGTGNLYCRVRLVNIQYNGTVSDGPWSTVQSYAFGGHNNDLNWQVTTSYAEDG
                     KRKVVMAYFDGSLRQRQTVTKDNSTNTTVSAETFYDGQGRPAIQILPAPGISSIVAYN
                     QTLNLFNGQTPGSDPGVFFDLGSGSSTMSMDNGYGAALYYSAGNTEIAQGSNQNIPDA
                     GGFPYTVTQYTPDATGRIMAQSGVGQALKMGSNHETKYFYGTAAQEELDGLFGTDAGD
                     FTHYFKNMVRDGNGQSTVSYVDMHGRT"
     gene            complement(1617..>2149)
                     /locus_tag="VKQ52_15675"
     CDS             complement(1617..>2149)
                     /locus_tag="VKQ52_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88695.1"
                     /translation="NKPAKRMILYANHQSVAGRFRDYMGLQMADSSVLRMDRNYQASM
                     QGSFGDTAVLEVRHRTALPETGLPREEVQVRFNPGTDEPYEVVQLHRSLVPVTRERYQ
                     QEGALPGWAGKTVAVGDSLFFLVKEQTVFFRYRRISHDATGRLPFTVSDRIAAEMPGK
                     YRPVRTFADYVLTQQF"
BASE COUNT          458 a          637 c          572 g          482 t
ORIGIN      
        1 gtccggccat gcatgtcgac atagctcacc gtgctctgcc cgttgccatc gcgcaccata
       61 ttcttgaaat aatgggtgaa atcgccggca tcggtcccga aaagaccgtc cagctcttcc
      121 tgcgccgccg tgccgtaaaa atatttcgtc tcatgattac tgcccatctt cagggcctgt
      181 cccaccccgc tttgtgccat tatcctgccc gtcgcatccg gcgtatactg cgtcaccgta
      241 tagggaaagc cgccggcatc gggaatattt tggttggaac cctgggcgat ctccgtgttc
      301 cccgcgctat aatacagggc ggcgccatac ccattgtcca ttgacatagt cgagctaccc
      361 gagcccagat cgaaaaacac accgggatcg gaacccggcg tctggccatt gaacagattg
      421 agcgtttggt tgtaagcaac tatcgaactg atacccgggg caggaaggat ctggatggcc
      481 ggccggccct gtccatcgta gaaggtctcg gccgacaccg tggtattggt gctgttgtcc
      541 ttcgtcacgg tctgccgttg ccgcaggctg ccgtcaaaat aggccatcac cacctttctc
      601 ttcccatcct ccgcgtagct ggtcgtcacc tgccagttaa gatcgttatt gtggccgccg
      661 aaagcatagg actgaacggt cgaccagggt ccgtcgctca ctgtgccatt gtattggata
      721 ttcacgagcc tcacccggca atagagattg cccgtaccat cgtaatacag cgggatattg
      781 tacccggtgg tccccaacgg caggctcacc cgggtggaat tgttccggaa cagcaggtcg
      841 taattgatag agccattctg cgtgtaaagc gacgccaatt cggtctccag ccaggcccat
      901 tccagttgca acccgttgtg tcccgtatat cccgcaggca ggctccattg caccggtagc
      961 tcgtccggca cccccgtagt cgccggcgta ttgacctgca aggtcggcgt tacgctgccg
     1021 ctgggggaga gaatataatc ccgccagata tgcatctcgt tacaaaccga caaaagactg
     1081 atgacagaca ctccattcga tagggagttc gcgggtacgt tcgtgatggt aacctgtaca
     1141 tactgcgccc cggtaaagct cagatactgt ttcgggttat aggatacccc tccatcctta
     1201 ctataactga tggtaaggat ctgggtcacc gacgtagtag ccgagggcga ggcgccatag
     1261 tccacctgca cggtcaccga agcctggaaa ttatccggga tataggtcgt ggcctcctcg
     1321 ttcagcttca gtgacactat attcgtgatc ttcaacggag tcttttcccc cgcggacgta
     1381 taggtcaggg gaatagtagc gcccgtggtc agtccgccgc catgcgtact ggacaggtag
     1441 ttgatagctt catcgtacac cggtcccgtc tttgccggat actgctgcga acgcaccgtc
     1501 ggggaaagac aaactccaat gcaaaatagc agcgcaagac ggaaggtcag gggaggtaag
     1561 aggcggctca ttttttttac ggctgtcatt tgctgaacgg tcattttggg atttggctaa
     1621 aattgctgcg tcaaaacata atcggcaaaa gtcctcacag gccggtattt cccgggcatc
     1681 tccgccgcga tgcggtcact cacagtaaat ggaagccttc cggtcgcatc gtgcgagatc
     1741 cgccgatacc ggaaaaagac cgtctgctcc ttcaccagga aaaagaggct gtccccgacg
     1801 gcaacggtct tgcccgccca tccaggaagc gcgccctcct gttggtacct ctcccttgtc
     1861 accggcacca ggctcctgtg cagttgcacc acctcgtaag gttcgtcggt accgggattg
     1921 aagcggactt gtacttcctc ccggggcagg ccggtctctg gcaacgcggt ccggtgcctg
     1981 acctccagca ccgcagtgtc cccaaaagat ccctgcatcg aagcctggta gtttctgtcc
     2041 atccgaagaa ccgacgagtc cgccatttgc aggcccatat aatcccgaaa ccggccggca
     2101 acggattgat gattggcata aaggatcatc cgtttggcag gcttattca
//
LOCUS       DATGFG010000831         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1182857, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000831 DATGFG010000000
VERSION     DATGFG010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182857"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VKQ52_15680"
     CDS             <1..647
                     /locus_tag="VKQ52_15680"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HLZ88696.1"
                     /translation="MHDSFTPLTGMFALWGMMINSFYGGVGVGFLNFYIFIIIAVFIS
                     GLMVGRTPEFLGKKIEAKEMKIATIIALLHTFLILAFTALSSHLYAHDPKTYAGWLNN
                     PGFHGFSEMLYEYTSSSANNGSGFEGLGDGVPFWNITCGLVMLLSRFLPIIGPVAIAG
                     ILAKKKYIPESAGTLKTDTGTFGVMTFAVIIIVAALSFFPALALGPIAEFFTMH"
     gene            658..2703
                     /gene="kdpB"
                     /locus_tag="VKQ52_15685"
     CDS             658..2703
                     /gene="kdpB"
                     /locus_tag="VKQ52_15685"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HLZ88697.1"
                     /translation="MSNYKRSNKLFEPALVKEALKHSFVKLNPVILFRNPVMFTVEIG
                     TVIMLFVCIWIMAGEHSQGSLVYNLLVFAVLFITILFANFAEAIAEARGKAQADSLRR
                     TREDTPAKKVFAVGEIYVNEVKTVPSSQLRKGDIFICETGDTIPMDGEIIEGLATIDE
                     SAITGESAPVIREAGGDKSSVTGGTKVLSDKIKVRVTTEPGESFLDKMIALVEGASRQ
                     KTPNEIALTILLAGFTLIFVIVCVTLKPFGDYANTPITIAAFISLFVCLIPTTIGGLL
                     SAIGIAGMDRALRANVITKSGKAVETAGDLDTLLLDKTGTITIGNRKATHFWPAAGVE
                     QKAFIEACILSSLADETPEGKSILELANANPGSYSVNGARFIGFTAETRCSGVDISGM
                     QIRKGAVDAIRSIVNRAGNSIPVGIEQKVKEIASNGGTPLAVSEGGRAMGVIELQDII
                     KPGIQERFQRLRKMGVKTVMVKGDNPLTARYIAEKAGVDDFIAEAKPEDKMNYIRKEQ
                     QTGKLVAMMGDGTNDAPALAQADVGVAMNSGTQAAKEAGNMVDLDNDPTKLIEIVEIG
                     KQLLMTRGTLTTFSIANDVAKYFAIVPALFITSIPGLQALNVMNLHSPESAILSAVIF
                     NAIIIPLLIPLALRGIAYKPIGASALLRRNLLIYGLGGVIVPFIGIKLIDLVVSIWF"
     gene            2723..>2922
                     /locus_tag="VKQ52_15690"
     CDS             2723..>2922
                     /locus_tag="VKQ52_15690"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF014699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HLZ88698.1"
                     /translation="MKAHLLTALKLTLACIVVFCGIYPLLILAIAQAAPAHGEGETVT
                     AGGKIAGYSLEGQNFTRDDYFWG"
BASE COUNT          645 a          774 c          852 g          651 t
ORIGIN      
        1 caatgcatga cagttttacg ccgcttaccg gcatgttcgc gctttggggg atgatgatca
       61 actcctttta cggaggggtt ggcgtcgggt tcctgaactt ctatattttc atcatcatcg
      121 ccgtgttcat cagcgggctg atggtgggac gaacgccgga attcctgggc aagaagatcg
      181 aagccaagga aatgaagatc gccaccatta tcgcgctttt gcacaccttc ctgatccttg
      241 ctttcacggc cctctcctcg catctgtatg cccatgaccc gaagacctat gcaggctggt
      301 tgaacaaccc cggttttcac gggttttcgg agatgttgta cgaatacacc tcctcgtcgg
      361 ccaataacgg ctccggattc gaagggctcg gagatggcgt tcccttttgg aatatcacct
      421 gtggcctggt gatgttgctg tcccggttcc ttcccattat cgggccggtg gcgatcgccg
      481 ggatactggc gaagaaaaaa tatattccgg aaagcgccgg aacgttgaag accgataccg
      541 gcaccttcgg agtgatgacc tttgcggtga tcatcattgt agcggcgttg tccttcttcc
      601 cggccctggc cctggggccg atagcggaat ttttcaccat gcattaaatc aaaggctatg
      661 tccaactata aaagatcaaa taagttattc gagccggcgc tggtcaaaga ggcattgaaa
      721 cattcctttg tcaagttgaa ccccgtcatc ctgttccgca acccggtgat gttcacggta
      781 gagatcggga cggtcatcat gttattcgtc tgcatctgga tcatggcggg cgaacattcc
      841 caggggagcc tggtgtataa cctgctcgtt tttgcggtgc ttttcattac gatcctgttc
      901 gccaatttcg cggaggccat cgcggaggcg aggggcaagg cccaggccga cagcctgcgc
      961 cggacgaggg aagatacccc cgccaaaaaa gtattcgccg tgggcgagat ctatgtaaat
     1021 gaagtgaaga ccgttccttc ctcgcagctg aggaaaggcg atatctttat ctgcgagacg
     1081 ggtgatacca tcccgatgga cggggagatc atcgaggggc ttgctaccat cgacgagagt
     1141 gctattaccg gcgagtcggc gcccgtcatc agggaggccg gcggcgataa gtcttccgtc
     1201 acgggtggta cgaaggtgct tagcgacaag atcaaggtgc gggtcaccac cgagccgggg
     1261 gaaagcttcc tggataagat gatcgcgctg gtggaaggcg ccagccggca aaagacgccg
     1321 aatgaaattg cgctgacgat cctgctggcc ggtttcacgc tcatctttgt catcgtatgc
     1381 gtaacattga aaccgttcgg ggactatgcc aataccccga ttaccatcgc cgcctttatc
     1441 tcgctcttcg tatgcctgat ccctactacg atcggcgggc tgctcagcgc gatcggtatc
     1501 gccggaatgg acagggccct gcgcgccaat gtcattacca agagcggcaa agcggtggag
     1561 acggccggcg acctggacac cctgctgctt gacaagaccg gtacgataac catcggcaat
     1621 cgcaaggcta cgcatttctg gccggccgcg ggtgtcgagc agaaggcctt tatagaagcc
     1681 tgtatactct cctcgctggc cgatgagacg ccggagggaa aatccatact ggagctggca
     1741 aatgccaatc ccggcagtta tagcgtcaac ggggcgcggt tcattggctt tacggcggaa
     1801 acccgttgca gcggggtgga tatctccggt atgcagatcc ggaaaggggc ggtagatgct
     1861 atccgctcga tcgttaaccg ggcgggtaat tcgatccccg tcgggataga gcagaaggtg
     1921 aaggagatcg cctccaacgg aggtactccg cttgcggtga gtgagggtgg ccgcgccatg
     1981 ggcgtcatcg aattgcagga tatcatcaag cctggtatac aggaacggtt ccagcggctt
     2041 cggaaaatgg gtgtaaagac agtgatggtg aagggtgata acccgctgac ggcaaggtat
     2101 atcgccgaga aggcgggggt cgatgacttc atcgccgaag ccaagccgga agacaagatg
     2161 aattatatca ggaaggaaca gcagaccggg aaactggtcg ccatgatggg cgacggcacc
     2221 aatgacgccc cggccctggc gcaggccgat gtcggggtgg cgatgaacag cggtacccag
     2281 gcggcaaagg aggccgggaa tatggtggat ctcgacaacg atccgaccaa actgatcgag
     2341 atcgttgaga tcggcaagca gttactgatg acgaggggta cgcttacgac cttctccatc
     2401 gccaatgacg tcgccaaata cttcgccatc gtgcccgccc tgttcattac ctcgatcccc
     2461 ggtttacagg cactgaatgt catgaacctg cacagtcccg agagcgccat tctttcggcg
     2521 gtgatcttca acgccatcat catccccctg ctgataccgt tggcgcttcg cggcatcgcc
     2581 tataagccca tcggtgcaag cgcgttgttg aggcgcaatt tgctgatcta tggcctgggg
     2641 ggagtgatcg ttcccttcat cggcatcaaa ctgatcgacc tggtcgtatc catatggttt
     2701 taataaatta ttatctatca tcatgaaagc gcatttatta acggcactca aactcaccct
     2761 tgcctgcatc gtcgtttttt gcggcattta tcctttgctg atcctggcga ttgcccaggc
     2821 agcgcctgcc catggggagg gagaaacagt tacggccggc ggaaagatcg ccggttattc
     2881 gctggaaggg cagaacttta cgcgcgacga ctatttctgg gg
//
LOCUS       DATGFG010000832         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_535087, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000832 DATGFG010000000
VERSION     DATGFG010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535087"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VKQ52_15695"
     CDS             complement(<1..225)
                     /locus_tag="VKQ52_15695"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ88699.1"
                     /translation="MPRKIKVACLVLSLLLCTCTVFAQGKVTGRVINKADDQPVPGAT
                     IQLKGAKVVTQSGTDGSFSIDLPGNSGTLVI"
     gene            complement(354..1520)
                     /locus_tag="VKQ52_15700"
     CDS             complement(354..1520)
                     /locus_tag="VKQ52_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88700.1"
                     /translation="MTKALLFLMGLLMAAGAFAQPVGIFENHLDIGNPKLHGDATYDE
                     TTQVYTIKGGGANIWFNRDEFQFVYKKIKGDFILTADFEFTGDTANAVGHRKTGWMIR
                     ESTDEGAASANACKHLDGLVVLQWRPYRGMFMRDPEEERFYPKKGGQTIRLERIANLI
                     TMKIAHPGEPLQLVGSCTTDALGKDVLVGLYICSHDSDKIAGARVWNVRIDKPVIHEY
                     SSNPHVTPEIAHDVLGSRLEIVDIADGARKVIREAPGPISRTANDHEGVERPANGKFL
                     YYNANLTGTMQIWRMHPDGSGKEQLTFDECHNWFPTVSPDGKWLAWLSFPMDINPDTA
                     PAHHEAMLRLMPLTTPGAPRVLAYLYSGQGTLNAPSWSPDSKHLAFISNSEPRP"
     gene            complement(1532..>2784)
                     /locus_tag="VKQ52_15705"
     CDS             complement(1532..>2784)
                     /locus_tag="VKQ52_15705"
                     /inference="COORDINATES: protein motif:HMM:NF037222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88701.1"
                     /translation="TGGNEGHARAELYNAMRDNVAALDGTRPFIPCSSGFSKAPTDWK
                     GSWPDDRPAGVYSGGPYSWQDDAQYYRLVDAGKDWVFKDETGIPSQPPYNTLSKIIPN
                     LVPDNNLPYPLNDTWGYHDACTGNGHYDTYYQAMLDRFGKPTGIRDFSDKMQLLNAGG
                     YRGIFEAAGHKLNETGGVMLWKLNAAFPSVVWQIYDWYLEPNAGYYFMQRACEPVHIQ
                     LNLDDSTIAVVNRTYLKRPGLRYHVEVVGMTGATLLKQEGQVSLDTTEVKEVLPVRSL
                     LRQTQGISFVLLQLRDKEGRTLSRNVYWMSPGHDFADLRQMPSAHVQATVRSSEKKDG
                     YAGWTIRFTNVSTRLAFFLNPQVIAGGEEVLPAYWSDNYFSIPAGESITVKVSCPTAA
                     LAGRTPQLRLEGWNIPPADLQLGP"
BASE COUNT          525 a          746 c          871 g          642 t
ORIGIN      
        1 gatgacgagc gttccgctat tgccggggag atcgatggag aaggagccgt cggtgccgga
       61 ctgggtgacg accttggcgc cttttagttg tatcgtcgct ccgggaacag gctgatcatc
      121 tgctttattg atgacccttc ccgtaacctt tccctgagcg aagactgtgc aggtacaaag
      181 cagaagactg agtaccaggc aggccacttt tattttcctt ggcataagca atcgttttta
      241 tcagacaaaa ctatatgcaa ggttcttcaa gtgctatttt tccaccacat caacactaca
      301 tcagatgctt cccgatgccc gtcgatcggg cttttcgcca catcttcgct acatcagggc
      361 cggggttcgg aattactgat gaaggcaaga tgtttgctgt ccggcgacca ggagggggcg
      421 ttgagcgtgc cctggccgct atagagatat gcgaggaccc ttggggcgcc cggcgtcgtc
      481 agcggcatca ggcggagcat cgcctcgtgg tgggcgggag cggtatcagg atttatatcc
      541 atgggaaagg agagccaggc cagccatttg ccgtcgggag agacggtggg gaaccagttg
      601 tggcattcgt cgaaggtgag ctgttctttt cccgagccgt cggggtgcat ccgccagatc
      661 tgcatggtac cggtgaggtt ggcgttatag tagagaaact ttccgttggc cgggcgttcg
      721 acgccttcgt ggtcattggc ggtcctgctg atggggcccg gtgcttcccg gatcactttc
      781 cgggcgccgt cggcgatatc gactatttcc aggcggctgc cgaggacatc gtgcgcaatc
      841 tccggcgtga cgtgggggtt ggagctgtat tcgtggatca ccggtttgtc gatgcggacg
      901 ttccacaccc gtgcaccggc aatcttatcg gagtcgtgcg aacagatata gaggccgacc
      961 agcacgtctt tcccgagggc gtcggtcgta caggagccga ccagctgcag cggttcgccc
     1021 ggatgggcga tcttcatcgt gataaggttg gcgatgcgtt cgagccggat ggtctggccg
     1081 cctttttttg gatagaagcg ttcttcctcg ggatcgcgca tgaacatccc gcgatagggc
     1141 cgccactgga gaacgacgag cccgtcgagg tgtttgcagg cattggcgct ggccgctcct
     1201 tcatcggtgg attcccggat catccagccg gtcttgcggt gaccgacggc attggcggta
     1261 tcgccggtga actcaaagtc ggcggtgagg atgaaatccc cttttatctt tttgtagaca
     1321 aactggaatt cgtccctgtt gaaccagatg ttggcgccgc cgccttttat agtatagacc
     1381 tgggttgtct catcataggt ggcgtccccg tgcagtttcg gattgccgat gtccagatgg
     1441 ttctcgaaga tacctaccgg ttgggcgaag gctcctgccg ccatcaggag acccatgaga
     1501 aagagcaatg cttttgtcat atgttgagcg attaaggacc caattgcagg tcagccggtg
     1561 ggatattcca tccttccagc ctcagctgcg gggtccggcc ggccaatgcc gccgtcgggc
     1621 agctaacctt taccgtgatg gactcgcctg ccggaatgga aaaataatta tcggaccagt
     1681 acgccggcag cacctcttcc ccgcccgcga tcacctgcgg gttcaggaaa aaggcaagcc
     1741 gtgtggagac attggtaaaa cggatcgtcc aaccggcata gccgtccttt ttttccgagg
     1801 atcgtacggt ggcctgtaca tgggccgaag gcatctggcg cagatcggca aaatcatggc
     1861 cgggggacat ccaatacaca ttccgggaca gggttcttcc ttccttatcc cttaactgca
     1921 gcaggacaaa ggagatgccc tgtgtctgcc ggagaaggct tctcaccggc aagacctctt
     1981 tcacctccgt cgtatcgaga ctgacctggc cctcctgttt taacagcgtg gcgcccgtca
     2041 ttccgaccac ttccacatgg tagcggaggc cgggtctttt caggtaggtc cggttgacga
     2101 cggcaatggt ggagtcgtcg aggtttagct ggatatgtac gggctcacag gctctttgca
     2161 tgaaataata gcctgcattg ggttcgagat accagtcgta gatctgccag acgacgctgg
     2221 ggaaggcggc gttgagtttc cagagcatga cgccgcccgt ctcgttcagt ttgtgccccg
     2281 ccgcctcgaa gatgccccgg tagccgccgg cattcaggag ttgcatcttg tcggagaaat
     2341 cgcggatgcc ggtcggtttc ccaaagcgat cgagcatggc ctgataatag gtatcgtagt
     2401 gtccgttgcc ggtgcaggcg tcgtgataac cccaggtatc gttcaaggga tagggcaggt
     2461 tgttatccgg caccaggttg gggatgatct ttgaaagtgt gttgtacggc ggctgggaag
     2521 ggatccccgt ctcgtccttg aagacccagt ccttgccggc gtcgacgagc cggtagtatt
     2581 gtgcatcgtc ctgccaggaa taggggccgc cgctgtagac gccggcgggc cggtcatcgg
     2641 gccaggagcc tttccagtcc gttggcgctt tcgagaaacc ggaggagcag gggatgaagg
     2701 gccgggtgcc gtcgagagcg gccacgttgt cacgcatggc attgtagagt tcggcgcggg
     2761 cgtgtccttc gttcccgccg gtcc
//
LOCUS       DATGFG010000833         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_441798, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000833 DATGFG010000000
VERSION     DATGFG010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441798"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VKQ52_15710"
     CDS             <1..244
                     /locus_tag="VKQ52_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88702.1"
                     /translation="PASSGIALRSARTERGLRMDFPGFPFLGIWAAPNADFVCLEPWC
                     GIADSVDSSQQWTEKEGINRLGAAERFERTWTLTVF"
     gene            complement(286..930)
                     /locus_tag="VKQ52_15715"
     CDS             complement(286..930)
                     /locus_tag="VKQ52_15715"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HLZ88703.1"
                     /translation="MAVTTEILLKLALSILLGGMLGAEREYRSKSAGFRTLTLICVGA
                     TIFTIFSQIIGGSGNPDRIASNIVTGIGFVGAGVIFKGDSTSKVNGITTAAMIWVTAA
                     LGMAIGAGYWLVAVIATVMVLVVLFLFSLVEDRIDRANQLRKYRIVCPYENETLHRFE
                     ALFTTHHLRFKRTRQGKSAENMISGEWLVQGTEQNHRHVIHELMRDPTVKALEF"
     gene            complement(940..2145)
                     /locus_tag="VKQ52_15720"
     CDS             complement(940..2145)
                     /locus_tag="VKQ52_15720"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HLZ88704.1"
                     /translation="MPTIPERIKQFNSSRLPEYTAMKYQIMAEDPFRFFRGTCHLFYE
                     DLNKAPSFPVSPTTWICGDLHLENFGTYKGDNRLIYFDLNDFDEGILAPAAWEVVRMM
                     ASIFTGCQSLNIDKKETRQIAELFLRVYTKHLSSGKSRYVEEETATGIVRTFLERICQ
                     RKQKELVRQRTEEGKNGRLQLRIDRVRFFPIDKTLRKDLTTHVNQWMQHSDVVKDRYQ
                     VIDACFRVAGTGSLGVRRYVFLARNTKDPRKHLLIDMKEALPSSVQPWTNIPQPAWAS
                     EAERVVAIQLRMQNINPALLSTTIFQGVPYVLKEMQPTADKIDFLTVRDRYKDIACVV
                     EDMAFLTASAQLRSAGRQGAAVPDELIEFGKSQHWQEPLLQYSAAYAAQVKKDYQDYF
                     TAYKAGYFS"
     gene            2199..2693
                     /locus_tag="VKQ52_15725"
     CDS             2199..2693
                     /locus_tag="VKQ52_15725"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HLZ88705.1"
                     /translation="MQGYTRTRSMLKWTGIAVIFLWAVGCRQPEPPEYYGFQDIQVAK
                     AAGGQATLSTVVKLYNPNPYNLQLKRAEVDVLINGKHAGHSLLDSTVFIPRRDTFFVP
                     ISVQIDMRSIFSNALSLLQSGQVNVALDGRVKVKKGMMTFNRPFHFESKEDLNSLLPS
                     GSGF"
     gene            2712..>3484
                     /locus_tag="VKQ52_15730"
     CDS             2712..>3484
                     /locus_tag="VKQ52_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88706.1"
                     /translation="MSTIRRQSIISSGLVYFGFALGFVYTILFAKGFTPAQYGLTSIF
                     LALGTIMFYVGNLGVPTYIYKFFPYYRDRLPREKNDMITWALLTGAVGFLLVTVGGLV
                     FKGLVIRKFSEQSPEFVKYYYLIFPFGFGLTFYSLLEAYAWQLKRSVLTNFFKEVQFR
                     MFTILLVLLFFAGVLKDFDIFIKLYAFSYLTIALTLFFYLVAIGEMHFTLKPSIVTRK
                     FFRKIVAQAGLVWSGQILYNLSLYFAQIVIAAVVPGGLK"
BASE COUNT          717 a          959 c          968 g          840 t
ORIGIN      
        1 tcccgcctct tccggtatcg ccctgcgctc ggcgcgcacc gaaagagggc tgcggatgga
       61 cttccccggc ttccccttcc tcggcatctg ggcggccccc aacgcggatt ttgtctgcct
      121 ggagccctgg tgcggcatcg ccgactctgt agacagcagc cagcaatgga cggaaaagga
      181 agggatcaat cgcctcgggg cggccgaacg gtttgaacga acctggacac tgacggtatt
      241 ctaaactgag gtgttctaag ctgaggtatg ctaagctgag gtatgctaaa actccagcgc
      301 ttttacggta ggatcacgca tcagctcgtg gataacatgg cggtggttct gctcggtgcc
      361 ctgcaccagc cattcccccg agatcatatt ttccgccgac ttaccctgcc gggttcgctt
      421 gaaacggagg tgatgggttg tgaagagcgc ttcaaaccgg tggagggtct cattctcgta
      481 tggacagacg atgcggtatt tgcgaagctg gttggcgcgg tcgatccggt cttcgaccag
      541 gctgaagaga aaaagcacga caaggaccat caccgtcgcg atgactgcta ccagccaata
      601 gcctgcgccg atcgccatac ccagcgccgc cgtcacccag atcatggccg ccgtggtaat
      661 cccattcacc ttgctggtgc tgtccccttt aaaaatgacg cccgccccca cgaacccgat
      721 acccgtaacg atattggagg cgatccggtc cggattgccg ctgccaccga tgatctggga
      781 gaagatcgta aagatggtgg cccccacaca aataagggtc agcgtgcgaa agcccgccga
      841 tttgctgcga tattcccgtt ctgcaccaag catgccgccg aggaggatcg acaaggcgag
      901 tttcaaaagg atttccgttg tgactgccat agtaggatgt taagaaaaat agccggcttt
      961 ataagcagtg aaatagtcct ggtagtcttt cttcacctgc gcggcatagg cggccgaata
     1021 ctgcagcagg ggctcctgcc agtgctggct cttcccgaat tcgatcagct cgtcgggtac
     1081 cgcggcgccc tggcggcccg cactgcgcag ttgcgcgctg gcggtgagga aggccatgtc
     1141 ttcgacgacg caggcgatat ccttgtacct gtccctgacg gtgaggaaat cgatcttgtc
     1201 cgctgtgggc tgcatctcct tcagcacata gggaaccccc tggaagatcg tagtgctcag
     1261 cagggcaggg ttgatattct gcatccgcag ctggatggct accacccgtt cagcctccga
     1321 ggcccaggcc ggctggggga tattggtcca gggttgtacc gacgagggga gggcctcctt
     1381 catatcgatg agcaggtgtt tcctgggatc ctttgtattg cgcgccagga acacataccg
     1441 gcgcacgccg agactgccgg tgcccgccac ccgaaaacag gcgtcgatca cctggtacct
     1501 gtccttcacc acatcggagt gctgcatcca ctggttgaca tgggtggtga ggtccttgcg
     1561 cagggtcttg tcgatgggaa agaaacgcac ccggtcgatc cggagctgca gcctgccgtt
     1621 cttcccttcc tcggtgcgtt gccggaccag ttctttttgc ttgcgctggc agatcctttc
     1681 caggaaggtg cggacgatac cggtggccgt ctcctcttcg acgtagcgtg acttgccgct
     1741 ggaaagatgt tttgtataga cacgcagaaa gagctcggcg atctgccggg tctccttttt
     1801 atcgatgttg agggactggc agcccgtgaa gatactggcc atcatccgga ccacttccca
     1861 ggcggcggga gcgaggatcc cttcgtcaaa atcattgagg tcgaaataga tcagccggtt
     1921 gtccccttta taggtgccaa aattctccag atgcaggtcg ccgcagatcc aggtggtggg
     1981 tgaaacgggg aaggacgggg ccttgttgag gtcctcatag aacaggtgac aggtgcctct
     2041 gaagaaccgg aaagggtcct ctgccatgat ctggtatttc attgccgtat actcgggtaa
     2101 ccggctgcta ttgaattgtt tgatccgttc gggaatagtg ggcacaagct ttcttttcag
     2161 aaaggtaagt aatattcagg aattcctatc ttgcaggtat gcaaggatat acaaggacca
     2221 gatcgatgct gaaatggacg gggatcgccg ttatattcct ctgggcggtg ggttgccggc
     2281 agccggagcc cccggagtac tatggattcc aggatattca ggtggcaaag gcggctggag
     2341 gccaggcaac gctctcgacg gtggtaaaat tgtacaaccc caatccctat aatctccagt
     2401 tgaaacgggc cgaagtggac gtcctgatca atggaaaaca tgccggtcat tcccttctcg
     2461 attccactgt ctttatcccc cggagggaca cgttcttcgt gcccatctcc gtccagatag
     2521 acatgcggag catcttcagc aacgccctct cgctcctgca aagcggacag gtgaatgtcg
     2581 ccctcgacgg cagggtcaaa gtgaagaaag gaatgatgac cttcaaccgg cccttccatt
     2641 tcgagagtaa agaagatctc aactccctgc tgccatccgg gtcagggttc taattatctt
     2701 atttttacca tatgagcacg atccggcggc aaagcatcat ctcttcaggc ttggtatact
     2761 ttgggtttgc gctgggattc gtctacacga tcctttttgc gaagggattt acaccggccc
     2821 aatacggtct gacaagcatc ttcctggccc tgggcaccat tatgttctat gtcggtaacc
     2881 tcggcgtgcc gacctatatt tacaaattct tcccttatta ccgggatcgt cttccccgcg
     2941 agaagaatga tatgatcacc tgggccctgc ttaccggggc ggtgggcttt cttcttgtca
     3001 cggtgggtgg tctcgtcttc aaaggcctgg tgatccggaa attcagcgaa caatcgcccg
     3061 aattcgtcaa atactactac ctgatctttc cttttggttt cggccttact ttttattcgc
     3121 tgctggaagc ctatgcctgg cagttgaagc ggtcggtgct gaccaacttt ttcaaggagg
     3181 tgcagttccg gatgttcact attctgctgg tgctgctttt tttcgccggt gtcctgaaag
     3241 actttgacat atttatcaaa ttgtatgcct ttagctatct caccattgcg cttaccctct
     3301 tcttctacct ggtggcgatc ggtgaaatgc attttaccct gaaacccagc atcgttacgc
     3361 ggaaattctt ccggaagatc gtcgcccagg cgggcctggt gtggagcgga cagatcctgt
     3421 ataatctctc gctctatttt gcccagatcg tcattgctgc cgtcgtcccc ggcggcctga
     3481 aata
//
LOCUS       DATGFG010000834         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_384051, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000834 DATGFG010000000
VERSION     DATGFG010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384051"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VKQ52_15735"
     CDS             complement(<1..1081)
                     /locus_tag="VKQ52_15735"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ88707.1"
                     /translation="MTKFQLTLRASLLACCCLLLLVSRAQQTDRRIRGTVKGPDGAPL
                     VGATVSLASDKKTATATDASGAFTLSLPAGATGAQELEVSSIGFETRRVSLQQGQLSV
                     AIVMDNSKSSLNEVVVTALGINREKRSLGYAVTTVKGDEFTTARENNIANALTGKVAG
                     VNAAGLSTGPGGSSRVTIRGNGTLGPDNQPLYVVNGMPIDNSVPGGAPTVNGITNNVD
                     RGDGIAGINPDDIESISVLKGGTAAALYGSRAANGVILITTKKGRAQRGVGLEYNSTA
                     TVENPAVIPDFQYTYGQGLDGVESTDLNSAQASGRKSWGALIDGNTNYVAVDGLHHPY
                     TAKKRNIQNFYQTGSTFTNSVAFSGG"
     gene            complement(1014..1418)
                     /locus_tag="VKQ52_15740"
     CDS             complement(1014..1418)
                     /locus_tag="VKQ52_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88708.1"
                     /translation="MNIHLLQDQTFPIFFLQSDGHGTVGFWFDHDSRRCFLGWIKIGY
                     WQRCAGVNLQFLQFLLKSSNVSPLLTEIVSHTANILQLFFSFQVFLTIFDWEFAKNAI
                     LPIPNLKTTAHDKIPTDPPGQPVGMLLPASPC"
     gene            1428..2111
                     /locus_tag="VKQ52_15745"
     CDS             1428..2111
                     /locus_tag="VKQ52_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HLZ88709.1"
                     /translation="MYNDLVNLIDVSEDTIRRDVNELAEEGQLIRIKGGAMSAAYHYG
                     HDAKTYAQSNKLVIAEKTLALLRDDIIVLIGGGTTVREFIKKIPNTLRATFITVNVLT
                     AVELLDKPMIKTIMIGGQLSTYSQMTVSGEVFEYLAGIRADLCLMGTNAIDSSAGLTD
                     SDWETIQVKKAMIKAAEKVAIIAISEKLNSSMQMKIADIEEIDYLITELSPDSQDLQS
                     YRSKNLTIL"
     gene            2141..>3486
                     /locus_tag="VKQ52_15750"
     CDS             2141..>3486
                     /locus_tag="VKQ52_15750"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HLZ88710.1"
                     /translation="MRITLVLPALLLSLSLSAQVRVSHLLTENLPDPLSVDSKSPRFS
                     WQLSSTDRNELQTAYELQVGDRPGDNAVWNPGKVSSDQSVFVVYDGPALQAGKRYFWR
                     VRVWDNKGRVSEWSKPAFWQMGMLAPADWKARWIQSSAKEEPSQPSPLLRTTFTAKSG
                     IASATAYITAHGLYEAQLNGQRVGDAFLTPGWTSYNKRLQYQAYDVTGLVHPGRNAVG
                     AMLGSGWYRGYICYDWQHNVYGSDVSLLFQLIIRYKNGRTDTVASDAGWKATAGEVRH
                     SEIYDGETIDHRQEKKGWSLPDYDDTGWAGVDTANYPKDNLVATINEPIMKQETFHAG
                     KLLTTPKGEKVIDFGQNLTGWVIVKVKGKAGDSIRLSHAEVLDKEGNFYTENLRRAKA
                     QDFYILKGGEEEVFEPHFTFHGFRYIRIEGSIGEINPDDYTAVALYSAMPKTGEFS"
BASE COUNT          819 a          924 c          995 g          748 t
ORIGIN      
        1 tcccaccgga gaaagcgaca ctgttggtaa aggtggaacc cgtctggtag aaattctgga
       61 tgttccgttt ttttgccgta tagggatggt gcaggccgtc cacggcaaca tagttggtat
      121 ttccatcgat cagcgcaccc caggatttgc ggcccgaggc ctgtgcgctg ttgagatcgg
      181 tggattccac cccatccagg ccctggccat aggtgtattg aaagtcgggg atgacggcgg
      241 ggttctctac cgtggcggta gaattgtatt ccagaccaac accacgctgc gccctgccct
      301 tcttggtcgt gatcaggatg acgccattgg cggcgcggct gccgtagagg gcggctgccg
      361 taccaccctt cagtacgctg atggattcga tatcatccgg attgatgccg gcgatgccgt
      421 ccccgcgatc cacgttgttg gtgatcccgt tgacggtggg tgcgccaccc ggaacggagt
      481 tatcgatggg catcccgttg acaacataga ggggttggtt atccggacca agggtcccgt
      541 taccccggat ggtcacgcgg ctggagccac ccggaccggt ggaaagaccc gccgcattga
      601 cgcctgccac cttgcccgtg agggcgttgg caatattatt ctcccgggcg gtagtgaact
      661 catccccttt taccgtcgtg acggcatagc ccaaggatct tttctccctg ttgatcccga
      721 gagcggtgac gacgacttca ttgagcgatg atttggaatt gtccatgacg atggcaaccg
      781 aaagctgtcc ctgctgaagg gatacccgcc tggtctcgaa gccgatagag cttacctcca
      841 gttcctgggc accggtggcg ccggcgggca ggctgagcgt aaatgcaccg gaagcatcgg
      901 tggcggtggc tgtttttttg tcggaggcga gagagacggt tgcgccgacc agcggtgcgc
      961 cgtcgggtcc cttcacggtg ccccttatgc ggcgatctgt ctgctgggcc cggctaacaa
     1021 ggagaagcag gcagcagcat gccaacaggc tggcccggag ggtcagttgg aattttgtca
     1081 tgagcagttg tttttagatt tggtatgggc agaattgcat tttttgcaaa ttcccaatcg
     1141 aatatagtga gaaaaacttg aaatgagaaa aatagctgca aaatatttgc agtatgtgat
     1201 acgatttcgg tcaataatgg gctgacattg cttgacttga gcaaaaattg caaaaactgc
     1261 aaattaaccc ctgcacaacg ctgccaatat cctatcttta tccatccaag aaagcatcgt
     1321 ctgctgtcat gatcaaacca aaagccaaca gttccatgcc cgtcgctttg caaaaaaaag
     1381 atcggaaaag tctgatcctg aagcaagtga atattcacac ccgtttgatg tataacgatc
     1441 tcgtcaattt gatcgacgtt tcggaggata ccatccgccg ggatgttaat gagttagccg
     1501 aagaaggcca gcttatccgg atcaaggggg gcgccatgtc ggctgcctat cattatgggc
     1561 atgacgcaaa gacctatgcg caatccaaca agctggtcat cgcggaaaaa acccttgcgc
     1621 tgttgcggga tgatatcatc gtgctgatcg gcggcggcac caccgtgcgg gagttcatca
     1681 agaagatccc caacacgctg cgggcgacct ttatcacggt gaatgtgctc acggccgtcg
     1741 aattgctgga caagccgatg atcaagacga tcatgatcgg ggggcagtta tccacttata
     1801 gccagatgac ggtcagcggg gaagtattcg aatatcttgc cgggatcagg gccgatctct
     1861 gtctgatggg gacaaacgcc atcgacagca gcgcagggct caccgactcc gactgggaga
     1921 cgatccaggt caaaaaggcc atgatcaaag ccgcagaaaa agtggcgatc atcgccattt
     1981 cagaaaaatt gaactcatcg atgcagatga agattgcaga catcgaagaa atagattatc
     2041 ttatcaccga gttgtcaccc gacagccagg atctccagtc ctatcgttcg aagaatctga
     2101 cgatcctctg atcccccaaa aaaagtcaaa cgctttggcc atgagaatta ccctggtatt
     2161 gcctgccctt ttgttatcgc tgtccctttc cgcccaggtg cgtgtttcgc atctcctcac
     2221 cgagaatctg cctgacccgc tcagcgtcga cagcaaatcg ccaagattca gctggcagtt
     2281 gtcgtcgaca gaccggaacg agttgcaaac ggcctatgag ctgcaggtgg gtgatcgccc
     2341 gggggacaat gccgtctgga atccgggaaa ggtctcctcc gatcaatccg tttttgtcgt
     2401 ctatgacggt cccgccctgc aggcggggaa gagatatttc tggcgggtac gggtatggga
     2461 taacaaggga cgggtctccg aatggagcaa gcctgctttc tggcagatgg gaatgctggc
     2521 acccgcggac tggaaggccc gctggataca atcgtcggca aaggaagagc cttcccagcc
     2581 aagccccctc ctccggacga cctttaccgc caaaagcggc atcgcatctg ccacggccta
     2641 tatcactgcc cacgggttgt acgaagcaca gctgaacggc cagcgggtcg gcgatgcttt
     2701 tcttaccccc ggctggacct cctataataa acgattgcaa taccaggcct atgacgtcac
     2761 cggcctggtc cacccgggca ggaacgcggt gggcgcgatg ctgggcagcg gctggtaccg
     2821 gggttatatc tgttatgact ggcagcacaa tgtctatgga agcgatgtat ccctcctgtt
     2881 ccaactgatc atccgctata agaacgggcg caccgatacc gtcgcgtcgg atgcgggctg
     2941 gaaggccacg gcaggcgaag tacggcattc ggagatctat gatggtgaga cgatcgatca
     3001 ccggcaggaa aaaaagggat ggagcctgcc cgattatgat gacaccggct gggccggcgt
     3061 ggacaccgcc aattatccaa aggacaacct cgttgccacg atcaacgaac cgataatgaa
     3121 acaggagacc tttcatgccg ggaaactgct gaccactccg aagggggaaa aggtgatcga
     3181 tttcgggcag aacctcaccg gctgggtgat cgtgaaggta aagggtaagg ccggcgacag
     3241 catccgccta tcccatgccg aggtattgga caaggaaggc aatttctata cggagaacct
     3301 gcgccgggca aaggcccagg acttctatat cctcaaaggc ggggaagaag aggtcttcga
     3361 accgcatttt acgtttcatg gtttccgtta tatccggatc gaagggtcca tcggggagat
     3421 aaacccggat gactatacgg ccgtcgcctt gtattcggcc atgcccaaaa cgggagagtt
     3481 ctcctg
//
LOCUS       DATGFG010000835         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_156808, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000835 DATGFG010000000
VERSION     DATGFG010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156808"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2031)
                     /locus_tag="VKQ52_15755"
     CDS             complement(<1..>2031)
                     /locus_tag="VKQ52_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88711.1"
                     /translation="GPEAEQFFNSIRLRNSIPAAWAGFSPARGGFTIDFPHSPTQNRN
                     SSTSDGIARWEYEATDTTTGDAFLLWKKTIQNYRFMEEDTADLALMEESFRNSDWIDR
                     VLSRRQAVYAGHACLNTVYQTKDGSFLRARFIVHGPDYYLLATRSRNRDRAFNSFFGS
                     LAFTPYRYPSLRNYVDTFIRISVTTPMVPDIDAGMRGIIERATSEEFLNSIPDYNNYW
                     PRPKTALFQDDSTGEAVYVSVESFPKYYYPKDTASFWLEETNEKRLRRDLIITSKHPW
                     RNGDESGYRYVLADTNTTRRIDMWVFLKGSHLFRIVSLTDSLQPGSDFLTRFYATIKP
                     LNLPTAGAASGNTPASPANPALSPVFESKLDLFFHDFYSKDSLLAKHAKEAIPNVYYG
                     PAGVPRLLDAIGNLPYNGKDYFETKTKFINELGYINDSAATPGVVAGLRKIYERAGDT
                     SLLQNAVLKALAHHKTAAAYHLLKQLMIQDPPVFDNAAEYTSLFRDIGDSLVLARTLF
                     PGLLQLATVDDYKSNIQALLTDLVDSSYLQAADYEPWFSQIYFDAKIQWKKQEGKDER
                     RLQKKEDDNDDNNEADGDETAGNELDNYAILLMPFYDSNPTIPHFFDKLLRSRDPLLR
                     MNTALLLLRHQRPVADSILLALAAGDATRCQLWQGLTAIGNADRFPHRFHTQE"
BASE COUNT          359 a          466 c          751 g          455 t
ORIGIN      
        1 ttcctgggtg tggaagcgat gggggaagcg gtccgcgttg ccgatggcgg tcaatccctg
       61 ccagagctgg cagcgggtgg cgtcgcccgc ggcgagcgcg aggaggatgg agtccgctac
      121 gggccgctga tgacgcagaa ggaggagggc cgtgttcatg cgcaggagcg ggtcacggct
      181 ccggagcaat ttatcgaaga aatgagggat ggtcgggttg gagtcgtaaa agggcatcag
      241 caggatggcg tagttatcca gttcgttgcc ggctgtctcg tcgccgtcgg cttcgttgtt
      301 atcgtcattg tcatcctctt ttttctggag gcgccgctcg tctttccctt cctgcttttt
      361 ccattggatc ttggcgtcga aatagatctg gctgaaccag ggttcgtagt cggcggcctg
      421 taaataactg ctgtcgacaa ggtcggtcag cagggcctgg atattggatt tatagtcatc
      481 cacggtggcc agctggagca ggccggggaa gagggtacgg gcgaggacga gggagtcgcc
      541 gatgtcgcgg aacaggctgg tatattccgc cgcgttgtcg aacaccggcg gatcctggat
      601 catcagttgt ttcagcaaat ggtaggcggc ggcagtcttg tgatgggcga gggctttcag
      661 gacggcattc tgcagcaggc tggtgtcgcc ggcgcgctca tagatctttc taagcccggc
      721 taccacgccg ggggtggcgg cggaatcgtt tatatagccc agttcgttta tgaatttcgt
      781 ttttgtctcg aagtagtcct tcccgttata ggggaggttc ccgatcgcgt cgagcagccg
      841 gggtacgccg gcggggccat agtatacgtt ggggatggcc tcctttgcgt gtttggcgag
      901 gagggagtcc ttgctgtaga agtcgtggaa gaagaggtcc agcttgcttt cgaacaccgg
      961 ggacagcgcg gggttcgcgg gggaggccgg ggtgttcccg gatgccgcgc cggccgttgg
     1021 caggttcagc ggtttgatgg tggcatagaa gcgggtgagg aaatcgctgc cgggttgcag
     1081 ggagtcggtg agggacacga tgcgaaagag atggctgcct ttcagaaaga cccacatgtc
     1141 gatgcggcgg gtagtgttgg tgtccgccag gacatagcgg tagccggatt catcgccgtt
     1201 gcgccaggga tgcttgctgg tgatgatgag atcgcggcgg agtctttttt cgtttgtctc
     1261 ttccagccag aaggaggcgg tgtccttcgg gtagtaatat tttggaaagg attccaccga
     1321 tacgtagacg gcttcgccgg tggagtcgtc ctggaagagg gcggtctttg ggcgtggcca
     1381 gtagttgttg tagtcgggga tggagttgag gaattcctcg ctggtggccc tttcgatgat
     1441 accgcgcatg ccggcgtcga tatccggcac catgggtgtg gtgacgctga tgcggataaa
     1501 ggtgtcgaca tagttccgga gggaggggta gcgataggga gtaaaggcga gggagccgaa
     1561 gaagctgtta aaagccctgt cgcggttgcg gctccgggta gccaacaggt agtagtcggg
     1621 gccgtgaacg atgaagcggg cacggaggaa ggagccatcc tttgtctgat agacggtgtt
     1681 gagacaggcg tgcccggcat agaccgcctg ccggcgggac agcaccctgt cgatccagtc
     1741 ggagttgcgg aagctttcct ccatcagggc gagatcggcg gtgtcttctt ccatgaagcg
     1801 atagttctgg atggtctttt tccagaggag gaaggcgtcg ccggtggtgg tgtcggtggc
     1861 ttcgtattcc cagcgggcga tgccgtcgga agtggagctg ttcctgttct gcgtgggaga
     1921 atgcgggaag tcgatggtaa agccgccgcg ggcggggctg aagccggccc aggcggcggg
     1981 aatgctgttg cggaggcgaa tggagttaaa gaactgttcg gcttccgggc c
//
LOCUS       DATGFG010000836         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1404572, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000836 DATGFG010000000
VERSION     DATGFG010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404572"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VKQ52_15760"
     CDS             complement(<1..1097)
                     /locus_tag="VKQ52_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ88712.1"
                     /translation="MPYVNRIGPFITLLFWLAAGVCRAQNTIGIPDIVNYARDVYNAG
                     TQNRGIVQDRNGIMYFANYQGLLSFDGNYWKNYPLPNKTVVRSVALGRDNRIYAGGQD
                     DFGYFSPDKSGKLVFTSLKSLLPEKNSSFTDIWFIVPYGNDVFFMSREMIFQLSNKMI
                     TVYPAASEWRFLGESNHQLVAQDGRNGLLEFRDGLWAPFLKESALPGNYLATCLFPFG
                     KDSSFVATVNTGFYILANNTITPFHFAGLNPFSNERVLTAISVNKDWLAIGTNLDGAY
                     IVNKQGEIIQNLSRKEGLQNNNILMLFLDRNKNLWMGLDNGIDFIAYNNAIKHIYPEK
                     LNEGLGYTSIIFNKELFVGTSNGLYSLPMDDR"
     gene            1323..2846
                     /locus_tag="VKQ52_15765"
     CDS             1323..2846
                     /locus_tag="VKQ52_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88713.1"
                     /translation="MNSKLSNALLLLVCLLVLSTGSATAQPLGIFDGHGDVGTNVKPG
                     SATFIPATGQYIITGAGYNVWADHDEFQYVWKKIKGDFILYTRAEFLGNWVNYHRKVG
                     WMIRKSLDGNSPHVNAVEHGDGLTSLQFRRTAGAQTEEHKFKLTKANILQLERKGDQY
                     IMRAAIYGEPYQTDTLSGVDLGDEVYVGLFVGSHNSDVLETGVFSNVNISVPYKGESG
                     GNTQMTLGSRLELLEIASGHRDVIYSVPYSIQAPNWAPDGKSLIFNDAKGAVYNFDLS
                     SRTPAPLNTGTVNHNNNDHVISFDGRMLGLSNFVKDLGGSIIYTVPITGGTPKQITPK
                     GPSYLHGWSPDGKDLVFCGERNGEFDVYKVPATGGKETRLTTTPGLDDGPEYTPDGQY
                     IYFNSVRSGLMQIWRMRPDGSGQEQVTTDSLNNWFAHISPDGKWFVYLSFLPEETPAG
                     IHPPYKHVYIRLLPIDGKGTPRVLAYVYGGQGSINTPSWSPDGKRIAFISNTDMGGN"
     gene            2931..>4568
                     /locus_tag="VKQ52_15770"
     CDS             2931..>4568
                     /locus_tag="VKQ52_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HLZ88714.1"
                     /translation="MKKHMRLSPAIAYAFLLLPVLLPAQALRQPAQHVRQVKLMHFTL
                     QSSAVVKAGGDSLSSARYTPANYWFPVTVPSTVLTGLVANKVYPDPYIGMNNMLIPDA
                     SDTFNREYHLEQYSYLPGEPNPWKKPYWYRTTFSVPATDRGRHFQLVFKGINYRAEVW
                     VNGRLLADSAKMVGMFEEFDLDATPAIHPGEENGLAVKIYPLDVPGEPATPQLKALGD
                     FYENGGPTGDIGKNVTMLSSVGWDWIPEVRDRNIGIWQPVYLRTTGQVVISKPRIITE
                     LPLPDTSLAKLTLELTLSNNETRPREGSVQVTITPENFTGEAITFSQKADLAAGSSGT
                     IRLSADKIPALLMHHPRLWWPNGYGQPWLYRIRLKYTDGGGLSDDTSFVFGVRTVNSS
                     TTTVNGWVRRDFFVNGKRIHLVGGAWVPDMMLNRDYQRYDYELRLCRNANVNLVRIWG
                     GGLGETDDFYELADRYGLLVWQDFWITGDTNGGFKGSADWPLQTSVFVDNVISTIYRI
                     RNHPSLLVWTGGNEGHAREELYNAMRDNVARLDGTRPF"
BASE COUNT         1077 a         1350 c         1158 g          983 t
ORIGIN      
        1 cggtcatcca tcgggaggga ataaagcccg ttggacgtac ccacaaaaag ctccttgtta
       61 aagatgatgg aagtataacc aagaccctcg ttcagctttt caggatagat atgcttgatg
      121 gcattgttat aggcgataaa atcgatcccg ttgtcaagac ccatccagag attcttgttc
      181 cggtcgagaa aaagcatcag gatattgttg ttttgcaacc cctccttccg cgacagattc
      241 tggatgatct ccccctgctt attgacgata taggcgccgt caagattggt cccgatggcc
      301 agccagtcct tgttcacgga aatagcggtc agcacccgct cattggaaaa tgggttcagg
      361 ccggcaaaat ggaagggggt gatggtatta ttggcaagga tataaaaacc ggtattgacc
      421 gtggccacaa aactgctgtc tttcccaaac gggaacagac aggtcgccag gtagtttccc
      481 ggcagcgcac tctcctttaa aaaaggggcc cacaggccgt cgcggaattc cagtaaccca
      541 tttctcccgt cctgggccac caattgatgg ttgctttccc cgagaaaccg ccattccgaa
      601 gccgccggat agaccgtgat catcttattg gacagctgga agatcatctc cctcgacatg
      661 aaaaagacat cgttcccata cggcacgatg aaccagatat cggtgaacga ggagtttttc
      721 tccggaagaa ggctcttcag ggacgtaaaa accagctttc cgctcttatc aggcgagaaa
      781 tacccgaaat catcctggcc cccggcatag atccggttat ccctgccaag ggccaccgac
      841 cggacgaccg ttttattggg caggggatag ttcttccagt aattgccgtc aaaactcagc
      901 agtccctggt aattggcgaa atacatgatc ccgttccggt cctgcacgat accccggttc
      961 tgcgtgccgg cgttatacac atcccgggca taattgacga tatccgggat accgatggta
     1021 ttctgcgccc ggcaaactcc ggcggccaac caaaagagga gggtgataaa gggtcctatc
     1081 ctgttgacat atggcataat ctgaccttcc ctataaagat agggatacct tatagaaaga
     1141 agtaccgatt tcagcccttg cggctttttg ttgtggcgtt gatgtggcaa aaacttgcta
     1201 atcaacgggg ttttttggac atgatgtagt atagatgtag tcgaaaagtg gtgatttgag
     1261 caatcgggat ataaatttgt cgtgctggtg acgccatatt aaaaacttca atcgctttcc
     1321 tgatgaactc gaaactgtct aacgccttac tactgcttgt ctgtttgctg gtcctttcca
     1381 ccggctccgc cactgcccag ccactgggca tttttgatgg acatggcgat gtaggaacaa
     1441 acgtcaaacc cggatcggcc acctttattc ccgcaaccgg acagtatatt atcaccggcg
     1501 ccggctataa cgtctgggcc gaccatgatg aattccaata tgtgtggaag aagataaaag
     1561 gggattttat cctgtacacc cgggcggaat tcctgggcaa ttgggtcaat taccaccgga
     1621 aagtgggttg gatgatccgc aagagcctgg atggcaactc cccccacgtc aatgcggtgg
     1681 aacacggaga tggcctcacc tcccttcagt tccgccgtac cgccggcgcc cagaccgagg
     1741 aacataagtt caaactgaca aaggccaata tcctgcagct ggaaagaaaa ggcgatcaat
     1801 atattatgcg ggcggccatc tacggtgaac cctaccagac cgataccctt tccggcgtcg
     1861 acctgggtga cgaggtctat gtagggcttt ttgtcggctc ccacaactcc gatgtcctcg
     1921 aaaccggggt ctttagcaat gtgaatatct ccgttcccta taaaggcgaa tccggtggaa
     1981 atacccagat gaccctgggc agcaggctcg aactgctgga gatcgcctcc ggacatcgtg
     2041 acgtgatcta ctccgtgccc tactccatcc aggcgccaaa ttgggctccg gacgggaaaa
     2101 gcctcatctt caacgatgcg aaaggcgccg tgtacaattt cgatctttcc tcccggacgc
     2161 cggcccccct gaataccggc accgtcaacc acaacaataa tgaccatgtc atttccttcg
     2221 acggcaggat gctcgggctg agcaacttcg taaaagacct gggtggttcc atcatctata
     2281 ccgttccgat aacggggggg acgccaaaac agatcacgcc aaaaggaccg tcttatctcc
     2341 acggctggtc acccgacggc aaagacctgg tcttctgcgg cgaacgcaac ggggagttcg
     2401 atgtctataa ggtgcccgcc accggcggaa aagagacccg cctgacaact acgcccggtc
     2461 tcgacgacgg tccggaatac acccccgacg gccagtacat ctacttcaat tccgttcgta
     2521 gcgggcttat gcagatctgg cgcatgcgcc ccgacggcag cggacaggaa caggtcacca
     2581 ccgacagcct caacaactgg tttgcccata tttccccgga tgggaaatgg ttcgtctatc
     2641 tttctttcct gccggaagag acacccgccg gcatccatcc gccatacaag cacgtctata
     2701 tccggctgct gcccatcgac ggcaagggta cccccagggt gttggcctat gtttacggcg
     2761 gacaggggtc catcaacacc ccctcctggt cacccgatgg aaaaagaatt gctttcataa
     2821 gcaataccga tatgggaggg aattaaattc gccttccgct gttacggctc ctggcaagtg
     2881 tgaatggcca cttgccggag ttcgtaactt tgcgatctca actgctcttc atgaaaaaac
     2941 acatgcgtct atccccggcc atcgcctatg cctttctcct gctgcctgtg ctcttgcccg
     3001 cgcaggccct aagacagccc gcgcagcacg ttagacaggt aaaactcatg cattttaccc
     3061 tgcaatcctc cgcggtggta aaggccggcg gcgactccct ctcctccgcc cggtacaccc
     3121 ccgccaatta ctggttcccc gtgaccgtgc cctctacggt attgacggga ctggtggcga
     3181 ataaggtcta tcccgatccg tatatcggga tgaacaacat gctgatcccc gatgcatcgg
     3241 acacctttaa ccgcgaatat catctggaac agtatagtta cctgccgggg gaacccaacc
     3301 cctggaaaaa gccatactgg taccggacaa ctttctccgt tccggccacc gacaggggcc
     3361 ggcattttca actcgtcttc aaaggcataa actatcgggc cgaggtctgg gtcaacggcc
     3421 ggctgctggc cgactccgca aagatggtgg gaatgttcga agagttcgat ctcgacgcga
     3481 ccccggcgat ccatcccggc gaagagaatg gcctcgccgt aaaaatatat ccactcgatg
     3541 tccccggcga acccgctacc ccccaattga aagccctcgg ggatttttat gagaatggcg
     3601 gaccaaccgg agacatcggc aagaatgtga ctatgctcag ctccgtcggc tgggactgga
     3661 tacccgaagt ccgggatcgc aatatcggca tctggcaacc ggtctatctc cggacgacgg
     3721 gacaggtcgt catcagcaaa ccccgcatca tcaccgaact gccgcttccc gacaccagcc
     3781 tggcaaaact cacgctcgaa ctcacgctgt cgaataacga aacccggccc agagagggct
     3841 ccgtgcaggt caccatcacc cccgagaact ttaccggcga agccatcacc ttctcccaaa
     3901 aagcggacct cgccgccgga tcgtccggca ccatccggct gtccgccgac aagatacccg
     3961 ccctgttgat gcatcacccc cgcctctggt ggccaaacgg ctatggacag ccgtggctgt
     4021 atcgcatccg gctgaaatat accgacggcg gcggcctctc cgacgacacc tcctttgtct
     4081 ttggcgtccg taccgtcaat tcttccacga caacggtcaa cggctgggtc agacgcgact
     4141 tctttgtcaa cgggaaaagg attcacctcg tcggcggggc atgggtgccc gatatgatgc
     4201 tcaaccgcga ctaccagcgc tatgactacg aattgcggct ctgtcgcaac gccaatgtca
     4261 acctggtccg tatctggggt ggagggctcg gcgaaaccga cgacttctat gagttggccg
     4321 atcgctatgg cctcctcgtc tggcaggact tctggatcac cggcgacacc aatggcggat
     4381 tcaagggctc cgccgactgg cccctgcaaa cctccgtctt tgtggataat gtcatcagca
     4441 ccatctatcg catccgcaat catcccagct tgcttgtttg gaccggcggc aatgaaggcc
     4501 atgcccggga agaattatac aacgccatgc gcgacaacgt agcgcggctc gacggcacgc
     4561 gacccttt
//
LOCUS       DATGFG010000837         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_68242, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000837 DATGFG010000000
VERSION     DATGFG010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68242"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..921)
                     /locus_tag="VKQ52_15775"
     CDS             complement(217..921)
                     /locus_tag="VKQ52_15775"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HLZ88715.1"
                     /translation="MKTLKIAFLAFFAIAAVTGRTFAQSEETRSVSGFNGLATGSSFA
                     VHVKIDGTESLRLKGSPEDLRRIETVVEHGVLKIRLPRSHDWDDERIGHVDVYVSAKS
                     LSLLEIGGSGSIDVEGVVKGDKVSVSVGGSGGISTAVESSDLHVSIAGSGNVKMSGKS
                     GDAKISVAGSGSLKAADLKVDEAEIGIAGSGDVRLEVEKTISAHIAGSGSVLYTGNAT
                     ISNVSSAGSGRVSRVK"
     gene            complement(1163..2959)
                     /locus_tag="VKQ52_15780"
     CDS             complement(1163..2959)
                     /locus_tag="VKQ52_15780"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HLZ88716.1"
                     /translation="MKKLLPAILLTLVWGGGAYAQSAMAVTSTEVNKIILGNYTPSTY
                     QATNIITDPGIISAELVNAISADSLKADLIALNNFQNRNTFSDTTSTTRGIGAARRWV
                     YNKFLQYSAANENRLRPAYLTFTYSPSSAGCSTSSITKHADVIAVLPGSQTANKSLII
                     IEGHMDSRNADNCDVTGSAAGIGDNATGTALVMELARVLSKYTFRNTILFSVNTGEEQ
                     GLVGATALAQYLQSNNVLIKTVNNNDVSGGTFCGHTSSAPSCPAYGNIDSTDLRIFSL
                     GDINSPNKQWARYVKLEYKEQVRSLVTIPTTIEVMEPEDRTGRGGDHQAFTKIGYTAV
                     RFTQANEDGNGSNGSGYSDRQHNIRDSLGHYNSSTNSIDSIYVNADYLARNAIVNGNS
                     LAMVALGPDTITMKGTLWASNSIKVQFTPTTSPGYRVAVRSATNDWDTVYTVTGASSA
                     ILNVPYASNTTFYVSAAAMDANGTESQFSTEYTLSTGLILLDLPPDTSRSVASDLNIY
                     GIQLMPNRPNPFDESTMIAITSGTDVFADRTVLQISGIDGRLISRIPVHLQKGLNQVV
                     FNHGFGAAGIYICSLIVDGLPVQSTKMVFRKP"
     gene            complement(3032..>3394)
                     /locus_tag="VKQ52_15785"
     CDS             complement(3032..>3394)
                     /locus_tag="VKQ52_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ88717.1"
                     /translation="NSPTPGTPNYYRLQWTDGNGDIAYSSVVKIASSLVSGVLDVNPN
                     PFRDQLTVRLSLNSTQRVAIRLLDSKGMLVQQGQYEGVKGVNTFTVEGLSSLPASVYL
                     VQIVLVDQIFVRKVFNTR"
BASE COUNT          671 a          880 c         1098 g          745 t
ORIGIN      
        1 tacctatacc tccgcggcca tgttccaata cctgaacggg gagatctacg aagggatcgg
       61 ggtgccgccg gatatctatg cccgggagac gcaggatgcc ttcaataaca agcacgacct
      121 ggtggtcgat gccgccatca agtatatcca ggcgcattag gccgggcggc aggccgcgac
      181 cgccgggcac cggcccctga gcggcggacg ccgcgtttat ttcaccctgc taacgcgacc
      241 cgaaccggca gacgagacat tgctgatggt ggcgttgccg gtatagagca cgctacccga
      301 accggcgata tgggccgaga tggtcttctc gacctccagg cggacgtcac cggagccggc
      361 gatgccgatc tcggcttcat cgactttcag atcggcggcc ttcaggctgc ccgaaccggc
      421 gaccgagatc tttgcgtccc cggattttcc cgacattttt acgttcccgg agccggcgat
      481 ggacacgtgc aggtcggacg attccaccgc ggtggaaatt ccccccgagc cacccacgct
      541 gacgcttacc ttatcccctt ttacgacgcc ttccacatcg atggaacccg aaccgccgat
      601 ttcgagcaag gagagggatt tcgccgaaac atacacatcg acatggccga tgcgctcgtc
      661 gtcccagtca tgcgaacgcg gaagccggat cttgagcaca ccgtgctcga cgaccgtttc
      721 gatcctgcga agatcttcgg ggcttccttt cagacggagg ctttcggtgc cgtcgatctt
      781 tacatgtacg gcaaaggagg agccggtggc gaggccgttg aagccggata ccgaccgggt
      841 ttcttccgac tgggcgaagg tcctgccggt gacggctgct atggcgaaga aggccagaaa
      901 ggcgattttg agcgttttca tttgctggtg ctttttattt tttgaacgtt ttaatgctga
      961 aaaggttact ctaagagtcg gggaatattg ggctttgttg catgggaagg gaacggaagc
     1021 ggcaataatt ccggatgggg caataaaaac aggcggagca acaaaaaggc ccccgccgta
     1081 cgggggcctt gcgcgtggag tcagggataa ggaagcaggt agtccgggtc atgcggcagg
     1141 cagcttggcc tccggcaata ctttatggct tacgaaagac catttttgtg ctttgtacag
     1201 gcaggccgtc gacgatcagc gagcagatat agataccggc cgcgccgaac ccgtggttga
     1261 aaaccacctg gttgagccct ttctggagat ggacggggat gcggctgatg agccggccgt
     1321 cgatgccgct gatctgcagt acggtacgat cggcaaagac atccgtcccc gaagtgatgg
     1381 cgatcatggt ggactcgtcg aagggattgg gccggttggg catcagctgg atgccataga
     1441 tgttcagatc gctggccacc gatcgactgg tgtcgggcgg caggtcgagg aggatcagcc
     1501 cggtgctgag ggtatattcc gtcgagaact ggctttcggt gccgttggca tccatggcgg
     1561 cggcgctgac atagaaggtg gtattactgg catacggaac gttcaggatc gcggaagagg
     1621 cgcccgtgac ggtatagacc gtatcccagt cattggtagc ggaacggacc gccacccggt
     1681 agccggggct ggtggtggga gtgaactgaa cctttatcga gttggaggcc caaagcgtgc
     1741 ccttcatcgt aatagtgtcg gggcccaggg ccaccatggc cagggagttg ccgttgacaa
     1801 tggcgtttcg cgcgaggtag tccgcattga cataaatgga gtcgatggaa ttagtcgagc
     1861 tgttgtaatg acccaggctg tcgcggatat tgtgctggcg atcgctgtag cccgagccat
     1921 tgctgccgtt gccgtcttcg ttggcctggg tgaagcgcac ggccgtatag ccgatcttcg
     1981 taaacgcctg gtggtcgccg cctctgcccg tgcggtcttc gggttccatc acctcgatag
     2041 tggtggggat ggtgacgaga cttctcacct gctccttgta ttccagtttg acatagcggg
     2101 cccactgttt attcggggag ttgatatcgc cgagcgagaa gatgcgcagg tcggtgctgt
     2161 cgatattccc gtaggcgggg cagcttggcg cggaagaggt atggccgcag aaggtgccgc
     2221 ccgatacgtc gttgttgttg acggtcttta tcagcacatt attgctctgc aggtattggg
     2281 ccagcgccgt ggccccgacg aggccctgtt cttcgccggt attgacggag aagaggatgg
     2341 tattgcggaa ggtgtacttg ctcaggacgc gggcaagttc catcacgagg gcggtgccgg
     2401 tggcgttgtc gccgatgccg gcggcgctgc cggtgacatc gcagttatcg gcattgcggc
     2461 tgtccatatg tccttcaatg atgatgaggg acttgttggc agtctgactg ccgggcagga
     2521 cggcgatgac atcggcgtgc ttggtgatgg agctggtgct gcaacccgcg ctggacggac
     2581 tgtaggtaaa ggtcagatag gccgggcgca gacggttctc gttggccgcg ctgtactgga
     2641 ggaatttgtt gtacacccag cggcgtgcgg ccccgatgcc ccgggtggtc gacgtggtgt
     2701 cggagaaggt gttcctgttc tgaaagttgt tgagcgcaat aagatcggct ttcagggagt
     2761 cggcggagat ggcattgacc aattcagccg agataatacc cggatcggtg atgatgttcg
     2821 tggcctgata cgtcgagggg gtataattcc ccaggatgat cttgttcacc tcggtcgatg
     2881 tgacggccat cgcggattgt gcgtaggcgc cgccccccca caccagggtg aggaggatgg
     2941 ctggcaatag ttttttcatg ccggcttgga tttacggtta atagcaattt tgtgaaaggg
     3001 tttttcaggt tagccggtcc ggtaggaaac gctaccgggt gttaaaaacc tttctcacaa
     3061 agatctgatc gacaagaacg atctgaacca ggtagacaga cgcgggcagg gaggacaggc
     3121 cttcgaccgt aaaggtgttg acacctttga cgccctcgta ttgtccctgc tgaaccagca
     3181 tccccttgct gtcgagcagg cggatggcca cccgctgggt gctgttgaga ctgagcctga
     3241 ccgttagctg atcccggaag gggttcggat ttacatccag gacgccgctc accagggaag
     3301 aggcgatctt cactacacta ctgtaggcga tatcgccgtt cccatctgtc cattgcaaac
     3361 gataataatt gggggtacca ggggtgggac tgtt
//
LOCUS       DATGFG010000838         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_547587, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000838 DATGFG010000000
VERSION     DATGFG010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547587"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2637
                     /locus_tag="VKQ52_15790"
     CDS             <1..2637
                     /locus_tag="VKQ52_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88718.1"
                     /translation="FVDAMPARISQIHLLLLFVLLLTGFRIAEPSKAISSPAPEISII
                     TDEAPGPIALHGLERLTSLMTSRHIAFETISDPARARGKYLLVAGLSDGNGAAAQWLK
                     AAGQGPARTAEAYILWHTIRQQKPALVISGYDDTGLMYALQETARQIGLGVNAPMALD
                     AMKDANEKPSIHTRAVSIYTMNRAYWESRFYDEKYWTRYLDELAENRFNSLVVVFGYE
                     NGGFLAPCYPYFFNVDGYPGIRMTGLSEAEQRRNLTAINRLIAMAHQRGIRFTAGIWD
                     HIYRGGVQGGGIPGNEKPPDHPVPGLVWGVTADNLTAYTKAALTAFIRQIPVDALQFR
                     MHGESGLKKEEEESFWADVFRMIRQVKPDLQIEMRAKELPPTVIRSAISTGIRFRIAT
                     KFWMEQFGLPYHPTRINPEKSYIRHSYGDLLRYPKEYNMDWRLWNGGTNRVLLWGDPD
                     YVRRFMKSVHLYDGDGFEINEPLATKMEAQPHDAAPFELLQPQHRYYEYEFERYWHLF
                     QVFGLIGYNPNTSSESWDKDFEARFGKTTGPLIERALHRASGILPRIIASCYPYSYFP
                     MTRGWAEKQRLGDLPTYAKAEGSDLCQFADFDDEAGLLLTGGETARIRPAENSQWFEQ
                     AARDVQTLINQVKDKGDTQNKELGSTLADLGILSNLALYHARRAPAAVYYCLYQRTHD
                     VSALDSAIAHERNAIDAWRQIVVSAADYYNDNLRMGVSVADLCGHWKDELGKLEDGLQ
                     KLEQQRQHADPGAMTVKAPAYKVSPAQPFDALFRVELLTPGEAMVNQSVNIRLRAAAP
                     LGIKWIRLFYRAVNQQLEYKDLPMEPDTTPGEYKAVIPVADIDPGYDLMYYIALMDKG
                     GHGRIFPDLNKETPYKIIKLIR"
     gene            complement(2641..>3301)
                     /locus_tag="VKQ52_15795"
     CDS             complement(2641..>3301)
                     /locus_tag="VKQ52_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88719.1"
                     /translation="HWGTQHMGLYTFATSLKHLGLYQKFGFWPRFLSIQMEKKVEKAG
                     ASPVANGVSGPIEMSWTKFSDVPAEGREGMLGRCRALTGKVFEGLDVGVDIRSVAEQG
                     LGETVLLWTRDELVGLAVCHCGAGTEAGSGICYIKFGAIYPALTGDLEFRRLLQACED
                     LAAKRGLEKVSGGINTARQEAWAAMLDSGWRGQMAGVAMQRPNEPGYNKPGVFLIDDW
                     R"
BASE COUNT          768 a          931 c          860 g          742 t
ORIGIN      
        1 tttgttgacg caatgcctgc ccggatatct caaatacatt tacttctcct cttcgtcctt
       61 ttgcttacag gcttccggat agcggagcct tcaaaagcga tctcgtcacc ggcgccggag
      121 atcagtatta tcaccgatga agcgcccgga cctatcgcgc tgcatggatt ggagagacta
      181 accagcctga tgacctccag gcatattgca tttgaaacga tatctgatcc cgcgcgcgcc
      241 aggggtaaat acttgctggt agcaggcttg tccgatggga atggcgccgc tgcccaatgg
      301 ctaaaggcgg caggccaggg ccccgcccgg acagcggaag cctatattct atggcatact
      361 atccggcagc agaaaccggc ccttgttatc agtgggtatg acgacacagg ccttatgtac
      421 gccttacagg agaccgcccg gcagatcggt ttgggcgtaa atgccccgat ggcgctcgac
      481 gctatgaaag acgccaatga aaaaccttcc atacataccc gggcggtttc catctatacc
      541 atgaaccgcg cttattggga aagccgcttc tatgatgaaa agtattggac ccgctacctg
      601 gacgaattgg ccgaaaatcg ctttaattct ctggtagtgg tttttggata tgagaatgga
      661 ggattcctgg cgccctgcta tccttacttt tttaacgtcg acggttatcc cggcatccgg
      721 atgacgggtc tcagcgaggc ggaacaacgc cgcaacctga cggcgatcaa tcggctgata
      781 gccatggcgc atcaacgggg tattcgtttc accgccggca tctgggacca tatctatcgg
      841 ggcggcgtac agggtggggg aataccgggt aatgagaaac caccggatca tcctgtaccc
      901 gggttggtgt ggggtgtcac cgccgataat ctcacggcat atacaaaagc agctttgacg
      961 gcattcatac gacagatccc cgtagacgct cttcagttca ggatgcatgg agagtccggc
     1021 ctgaaaaagg aagaagagga gtcattctgg gcggatgtgt tcaggatgat ccggcaggta
     1081 aagccggatc tgcagatcga aatgcgggcc aaagaacttc ctcccaccgt catacgaagc
     1141 gccatcagca cgggtatccg attcaggatc gccaccaagt tctggatgga gcaatttggg
     1201 ctgccgtacc atcctacacg catcaaccct gagaaaagct atatccggca cagttatggg
     1261 gacctgcttc gctatccgaa ggaatacaat atggactggc gattgtggaa tggcggcacc
     1321 aatcgcgtgc tgttgtgggg cgaccctgac tatgtccgcc gctttatgaa aagcgtgcac
     1381 ctgtatgatg gagatggatt cgagatcaac gagccgcttg ccaccaagat ggaggcccag
     1441 ccgcacgacg cagcgccttt tgaactgctg cagccacaac atcgttatta tgagtatgag
     1501 ttcgaacggt attggcatct tttccaggtc tttgggctca tcggctataa tcccaatacg
     1561 tcttccgaaa gctgggacaa ggatttcgaa gcccgttttg gaaagacgac gggcccactc
     1621 atagaacggg ccttgcatcg ggccagtggc atattgcctc gtatcatcgc ctcctgttac
     1681 ccttatagct attttccaat gacgcgaggc tgggctgaaa aacaacgcct gggcgatctc
     1741 cccacctatg cgaaggccga aggcagcgat ctgtgccagt ttgccgattt tgatgacgaa
     1801 gccggtcttt tgcttacagg aggtgagaca gcgaggatca gacccgctga aaacagtcaa
     1861 tggtttgagc aggctgccag ggatgtacaa acactgatca accaggtaaa ggataaaggg
     1921 gatacgcaaa acaaagaact gggttctacg cttgccgatc tgggtatctt atccaatctg
     1981 gcgctttatc acgctcgccg ggcgccggct gcggtgtact attgtctgta tcagcgcacc
     2041 catgatgtat cagcgctgga ttcggccatc gcacatgaac gcaatgccat cgatgcctgg
     2101 cggcagatcg tagtatcagc cgcggattac tataatgaca acctgcggat gggggtgtcc
     2161 gttgccgatc tttgcgggca ctggaaggat gagctcggca agctagagga tggcttgcaa
     2221 aaattggagc agcaacgtca gcatgccgat cccggggcga tgacggttaa agcaccggct
     2281 tataaggtct ctcccgcaca gccgtttgac gctttgttca gggtggaact gctgacaccc
     2341 ggcgaggcaa tggtcaatca gtctgtcaac attcgactca gggccgctgc gccgctgggc
     2401 attaagtgga tccgcttgtt ttaccgcgcc gtcaaccagc aattggaata taaagacctg
     2461 ccgatggagc cggataccac gccgggtgaa tataaggcgg tgatacctgt tgccgacatc
     2521 gatccaggct atgatctgat gtattatatt gccttgatgg ataaaggcgg ccacgggagg
     2581 atctttcccg atctgaataa ggagacgccg tataaaataa taaaacttat ccgttaaccc
     2641 ctacctccaa tcatcgatca aaaacactcc gggcttgttg tatcccggct cgttcggccg
     2701 ttgcatggcg acacccgcca tctgcccgcg ccacccgcta tccaacatcg cggcccaggc
     2761 ttcctgccgt gctgtgttga tgcctccgga gaccttctct aacccgcgct ttgccgccag
     2821 atcttcacat gcctgtagaa gtcgtctgaa ttccaggtcc cctgtcagcg ctggataaat
     2881 cgccccgaac tttatatagc aaattccgct gcccgcctct gtgcccgctc cgcaatgaca
     2941 aacggctaga ccgacaagct catcccgggt ccataataag acagtctccc ctaacccttg
     3001 ttccgcaaca gatcggatat cgaccccgac atccaaaccc tcgaacactt ttccggtcag
     3061 ggcccggcac cggcctaaca tcccttcccg tccctccgct ggcacatcgg agaacttcgt
     3121 ccacgacatc tctatcggtc ccgacacccc gttcgccacc ggcgacgcgc ctgctttttc
     3181 caccttcttc tccatctgta tgctcaaaaa tctcggccag aacccaaact tttggtacaa
     3241 tcccaaatgc ttcaggctcg tcgcaaacgt atataatccc atatgttgcg tcccccagtg
     3301 g
//
LOCUS       DATGFG010000839         5162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_987529, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000839 DATGFG010000000
VERSION     DATGFG010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987529"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VKQ52_15800"
     CDS             <1..513
                     /locus_tag="VKQ52_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88720.1"
                     /translation="VGQSKTPGYALGQLDVLDGFKVRLSDVDEHYINRIFTGQTGTFT
                     FNDSTYKLKIVKVFTQVTNGRFQVDMEFVGKVPHGIRKGQTLQITLALSDETTAVLVP
                     KGGFFQQTGGNWIFKVSEDGKTAYRVDIQLNRQNTDFYEVVSGLKPGDKVVTSSYENY
                     GNMQELVLKK"
     gene            575..751
                     /locus_tag="VKQ52_15805"
     CDS             575..751
                     /locus_tag="VKQ52_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88721.1"
                     /translation="MRLRILFTLGFVLLLSTFASSNECLRQHHAVECKGTTSPAPSIN
                     GEYSTLLVNKLLLI"
     gene            792..1469
                     /locus_tag="VKQ52_15810"
     CDS             792..1469
                     /locus_tag="VKQ52_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ88722.1"
                     /translation="MIKISELQKTYRTEEVETIALNKLSFEVKEGEFVSVMGPSGCGK
                     STLLNILGLLDDPDGGSFLFNGIEVAHFNERKRADLRKRNIGFVFQSFNLIDELTVFE
                     NVELPLIYLGVKGPERKQRVEAVLDKMQIMHRRNHYPQQLSGGQQQRVAVARAVVNNP
                     KLILADEPTGNLDSSNGNEVMTLLTDLNEQGTTIIMVTHSEHDSRYSHRIIRMLDGQT
                     VTENILI"
     gene            1620..4052
                     /locus_tag="VKQ52_15815"
     CDS             1620..4052
                     /locus_tag="VKQ52_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88723.1"
                     /translation="MLKNYLKIAFRNLAKYKVISFINLFGLTVGLTCCLLILTYILHE
                     ESYDKYNSKADRVWRVTRSFNNKDGIVSLHLGAVAPPFGPLLQNDFPDIEKITRLLPA
                     GSQPVRYEDKLFNEDNIFFADNNLFSIFDVTILKGDRTNALTDPLSVVMTETVAKRYF
                     GDADPINKVIRINNLYNLRVSGIIAPFPETAHFHPEILFSFNALKDSAIYGERNLATN
                     WGNNAFFTYLLLPPNYPAKSLEARFPAFLDRHMPSNKKDPASQPSRYTKLFLQNLTDI
                     HLHGHTDEEIETNGDSSRITIFAAIALFILLIACINYMNLSTARSTLRAKEIGIRKVS
                     GAQRYEIIYQFLSESVLISWLAIILAATLTTLALPWLNGLTGLHLSIQVLLRWYILVP
                     LILTPFVIGILSGIYPALFMSSFQPVKVLKGLFKAGSNTVNFRKVLVVMQFAISIVLL
                     ISTAIVFQQLHYMQNASLGFDKDHVLTLRYNNGLDKTFESFRTALGQDPHVLAASRSS
                     RIPSGRLLDEMGAQSESGDSLRPVNADIKYVAVDHDFVKTYGIPIVAGRDFSRNYATD
                     STGYLLNVAATKVLGLKTPQEMIGRNFAYGGVKGKVIGVMGDFNFEDMHQSILPIIFV
                     ILPASQNQFGRISIKLAGANLNTTMTHIEKTWKRYLPEVPFEASFLDQKFQKLYETEQ
                     QQGSLFTTFAGIAIFIACLGLLGLSAFAISQRIKEIGVRKVLGASTGSIVTLLSTDFL
                     RLVAIAALIAFPVAWYTMHNWLRDFAYRISIQWWVFLGAGIPAAAVALFTISFQAVRA
                     AISNPVKSLRSE"
     gene            4134..>5162
                     /locus_tag="VKQ52_15820"
     CDS             4134..>5162
                     /locus_tag="VKQ52_15820"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88724.1"
                     /translation="MFRNHLRIAWRGLLKRKGFSALNIFGLAIGITCCLLIFHYVAAE
                     RSYDTFQPNAGRIVRLRLDEYQQGKLAWQSATVYPAIGPAMKKEFPEVEQFGRLIDAD
                     LLLSNPEKNVKFNELKGYYADPSMLGMLGVRLLKGSPATALDAPDKILLSESMARKYF
                     GNEDPLSKRLRAQGWGTTEDYEVTGVFKEYPANSHLIINHLVSYSTINKIARSRGDST
                     NSSETAWGWYDFYVYLQLRPGANWKGLEGKLPDFSNRHMNNDEWHKKNNVRSELHLIP
                     LPDIHLYSNYNQEAEVNGNGKAVSFLFLIAFLIIAIAWINYTNLATARSLERAKEVGL
                     RKVLGAVRI"
BASE COUNT         1256 a         1849 c         1087 g          970 t
ORIGIN      
        1 gtaggccagt ccaaaacccc cggctatgcc ctcggtcaat tggacgtgct cgacggcttc
       61 aaggtccggc tctccgacgt ggatgaacac tatatcaacc gcatcttcac cggccagacc
      121 ggcaccttca ccttcaacga cagtacctat aagctgaaga tcgtcaaggt atttacccag
      181 gtgacaaacg gtcgcttcca ggtggacatg gaattcgtcg gaaaagtacc ccacggcatc
      241 cgcaaaggcc agaccctcca gatcaccctg gccctcagcg acgaaacaac ggccgtcctc
      301 gtacccaaag gcgggttctt ccagcagacc ggcggcaact ggatcttcaa ggtcagcgaa
      361 gacggcaaaa cagcctaccg ggtcgacatc cagctgaacc gccagaacac ggacttttac
      421 gaggtcgtct ccggcctgaa acccggcgac aaagtggtca ccagcagcta tgagaactat
      481 ggaaatatgc aagaactagt gctgaaaaaa taatatattg tacagcccgt aacagacgcc
      541 cttcaataag cgtctattac aaaaaaccaa ctatatgcgc ctgcgaatct tattcaccct
      601 cggcttcgta ctcctcctga gcacgttcgc ctcatcgaat gaatgcctcc gccagcacca
      661 cgctgtcgaa tgcaagggga caacctcacc agccccctcg ataaatggtg aatattcgac
      721 gctgctcgta aacaaactat tattaatata accgccatat tacacatcac actctaaaca
      781 gcttacactt tatgatcaag atcagcgaac tccaaaaaac ataccgcacg gaagaggtgg
      841 aaaccatcgc gctgaacaaa ctcagcttcg aagtcaaaga aggcgaattc gtttccgtca
      901 tgggcccctc gggctgcggg aaatcgaccc tcctcaatat cctcggcctg ctcgatgatc
      961 ccgatggcgg tagtttcctg ttcaatggca tcgaagtagc ccacttcaac gagcgcaaaa
     1021 gggccgacct ccgcaaacgc aacatcggct tcgttttcca gagcttcaac ctgatcgatg
     1081 agctgaccgt attcgaaaac gtcgaactcc ccctgatcta cctgggcgta aaaggccccg
     1141 aaagaaaaca acgcgtggaa gcggtcctcg acaagatgca gatcatgcac cgccgcaacc
     1201 actatcccca acaactctcc ggtggtcaac aacagcgtgt cgcagtagcc cgcgccgtgg
     1261 tcaataaccc aaaactgatc ctggcggatg aacctaccgg taacctggac tcgtcaaacg
     1321 gtaacgaagt gatgacactc ctcaccgacc tgaacgaaca gggtaccacc atcattatgg
     1381 taacccactc cgaacacgac tcccgctaca gccatcgcat catccgcatg ctggacggcc
     1441 agaccgtaac ggagaacata ttaatctagc gtccaacggc ggcgggccgg caaaccggct
     1501 ccgctatcgc ggcccgcgct accgcgcgca cgccgccgcc cggactcagt caacgccaac
     1561 caacggatcg caacttcgaa aatttttgta ctggccggta acggccaaac ctactcgtca
     1621 tgctcaaaaa ttatctcaag atcgccttcc gcaacctggc taaatacaag gtcatctcct
     1681 ttatcaatct cttcggactg accgtcggcc tcacctgctg cctgctcatc ctcacctata
     1741 tcctgcacga agaaagctat gataaatata acagcaaggc agacagggtc tggcgcgtca
     1801 cccgcagctt caataacaag gacggcatcg tctccctcca cctcggagcc gtggctcccc
     1861 ccttcggccc cctcctgcag aacgatttcc ccgacatcga gaagatcacc cgcctcctgc
     1921 ccgccggctc ccaacccgtg cgctacgagg acaaactctt caatgaagac aacatcttct
     1981 tcgcggataa caacctcttc agcatcttcg acgtcaccat cctgaaaggc gaccgcacaa
     2041 acgccctcac cgaccccctc tccgtcgtca tgaccgagac cgtggcaaaa agatatttcg
     2101 gcgacgccga ccctatcaac aaggtcatcc ggatcaacaa cctctacaat ctacgggtct
     2161 ccggcatcat cgcccccttc ccggaaacag cccatttcca ccccgagatc ctcttctcct
     2221 tcaacgccct gaaggactcc gccatctatg gggaaagaaa cctggccacc aactggggca
     2281 acaacgcgtt cttcacctac ctcctcctcc ccccgaacta cccggcaaaa agcctcgaag
     2341 cccgcttccc ggccttcctc gacagacata tgccctccaa taaaaaagac ccggcctccc
     2401 aaccctcccg ctatacaaaa ctcttcctcc aaaacctcac cgatatccac ctccacgggc
     2461 acaccgatga agagatcgaa accaatggcg acagcagccg catcaccatt ttcgccgcca
     2521 tcgccctctt tatcctcctc atcgcctgta tcaactatat gaacctctcc acggcccgct
     2581 ccaccctccg ggccaaagag atcggcatcc gtaaagtctc cggcgcccag cgctacgaga
     2641 tcatctatca attcctcagc gaatcggtcc tcatctcctg gctcgccatc atcctggccg
     2701 ccaccctcac caccctggcc ctgccctggc tcaatggact caccgggctc cacctgtcca
     2761 tacaggtcct ccttcgctgg tatatcctgg tgcccctgat ccttaccccc ttcgtcatcg
     2821 gcatcctctc aggcatctac cccgccctct tcatgtcctc cttccagccc gtaaaagtgc
     2881 tgaaaggcct gttcaaagcc ggcagcaata ccgtcaactt ccgcaaggtc ctcgtcgtta
     2941 tgcagttcgc catctccatc gtcctgctca tcagcacggc catcgtcttc cagcagctgc
     3001 actacatgca aaatgcttcc ctcggcttcg acaaggatca cgttcttacc cttcgctata
     3061 acaacggcct cgataagacc ttcgaatcct tccgcaccgc cctcgggcag gacccgcatg
     3121 ttctcgcggc aagccgctcc tcccgtatcc ccagcggccg cctcctcgat gaaatgggcg
     3181 cccaatcgga aagcggcgac tccctccgcc cggtaaacgc cgatatcaaa tacgtggccg
     3241 tagaccacga cttcgtcaaa acatatggca ttccgatcgt cgcgggccgc gacttctccc
     3301 gcaactacgc caccgacagc acaggctatc tcctcaacgt agccgctaca aaggtcctgg
     3361 gcctcaaaac cccgcaggaa atgatcggca ggaatttcgc ctacggcggc gtaaaaggca
     3421 aggtcatcgg tgtcatgggt gacttcaact tcgaagacat gcaccagtcc atcctgccca
     3481 ttatcttcgt catcctgccc gccagccaaa accaattcgg ccgcatctcc ataaaactcg
     3541 ccggcgcgaa tctcaacacc acgatgaccc atatcgaaaa gacctggaaa cgctacctcc
     3601 ccgaggtgcc gttcgaagcc tccttcctcg atcaaaaatt ccagaaatta tacgagaccg
     3661 aacaacaaca gggctccctc ttcaccacct tcgccggcat cgcgatcttt atcgcctgcc
     3721 tcggcctcct gggcctcagc gccttcgcca tctcccaacg cataaaagag atcggcgtcc
     3781 gcaaggtcct cggcgccagc accggcagca tcgtcaccct gctctcgacc gacttcctgc
     3841 ggctggtggc catcgccgcc ctcatcgcct tccccgttgc ctggtacacc atgcacaact
     3901 ggctccgcga cttcgcctat cgcatctcca tccaatggtg ggtctttctg ggcgcaggca
     3961 tcccggcagc agcagtagcc ctgttcacca tcagcttcca ggcggtgcgc gcggcgatct
     4021 ctaaccccgt gaaatccctg cggagcgaat gatcttggac actgcctggc aacccgtgaa
     4081 atcccttcga agcgaataag cggcccgcgg ccgccccaaa taaaaacctc cttatgttcc
     4141 gcaaccacct ccggattgcc tggcgcggcc tgctcaaaag aaaaggcttt tccgccctta
     4201 atatcttcgg cctggccatc gggatcacct gctgcctgct gatctttcat tatgtcgccg
     4261 ccgaacgcag ctacgacacc ttccaaccca acgccggccg catcgtccgc ctccggctgg
     4321 acgaatacca gcagggaaaa ctcgcctggc agtccgccac cgtctacccc gccatcggcc
     4381 ccgcgatgaa aaaggaattc ccggaagtag aacagttcgg ccgcctcatc gacgccgacc
     4441 tcctcctctc caaccccgaa aagaacgtaa aattcaacga gctcaaaggc tactacgccg
     4501 acccctccat gctcggcatg ctcggcgtca gactcctgaa aggcagcccc gccaccgccc
     4561 tcgacgcccc ggacaagatc ctcctctccg aatccatggc aagaaaatac ttcggtaacg
     4621 aagaccccct gagtaaacgc ctgcgcgccc agggctgggg caccaccgaa gactatgagg
     4681 tcaccggcgt cttcaaagaa tatcccgcca actcccacct catcatcaac cacctcgtct
     4741 cctattccac gataaacaag atcgcccggt cccggggaga ctccaccaac tcgtccgaaa
     4801 ccgcctgggg ctggtatgac ttctacgtct acctccaact ccgccccggc gccaactgga
     4861 aaggcctcga aggaaaactc cccgacttca gcaaccgcca catgaacaac gacgaatggc
     4921 acaaaaagaa caacgtccgc agcgaactcc acctcatccc cctccccgat atccacctct
     4981 attcgaacta taaccaggaa gccgaggtca acggcaacgg aaaagccgtc agcttcctct
     5041 tcctcatcgc cttcctcatc atcgccatcg cctggatcaa ctataccaac ctcgccaccg
     5101 cccgctccct ggaaagagca aaagaagttg gcctgcgcaa ggtcctcggc gccgtccgca
     5161 tc
//
LOCUS       DATGFG010000840         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_560599, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000840 DATGFG010000000
VERSION     DATGFG010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560599"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VKQ52_15825"
     CDS             complement(<1..1488)
                     /locus_tag="VKQ52_15825"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HLZ88725.1"
                     /translation="MAAEKLPDHSPTSLYSEDLAPIGREQRTWNTWNYAALWISMSLC
                     IPTYMLASSLIGGGMNWWQAIATIFLGNSIVLIPMILNGRAGAKYGIPFPVFARASFG
                     TKGANIPALLRAIVACGWFGIQTWIGGNALYQMLHLWVPALDRLPQLPAALGLATGPA
                     ICFLLFWLLNMYVVWLGVDSIRKLLVFKAFFLPLAALALLYWAVSHVHGGLGPILTQP
                     AKFPDSAAFWAFFIPGLTGMVGFWATLSLNIPDFTRYAVSQKAQVKGQALGLPTSMTL
                     FAFIGVVVTSATTIIYGTTIWDPVALAGRFDSKILVSIAMIAVAISTLATNIAANIVS
                     PANDFANLAPAKINFRTGGFITGIAGILIFPWKLVSDPTGYIFTWLVGYSSLLGPVGG
                     ILIVDYYFIRRQQLSVKDLYDPNGIYRFSAGINRKAVICLLLGILPNVPGFLVTVGAA
                     GPGSFPAWIVHLYSYAWFVGFAIAGGCYGLLMRADRQPITETHFNK"
     gene            complement(1505..2785)
                     /locus_tag="VKQ52_15830"
     CDS             complement(1505..2785)
                     /locus_tag="VKQ52_15830"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HLZ88726.1"
                     /translation="MLKRSTILPLCCGWLLLFQTAANAQPAATAGDIPAATAGQPKQA
                     TSPSAPIKRARDYGIPFDGAPGPLNAITDVKGVTVGQTTLISGDGPLVKGKGPIRTGV
                     TAILPRGKEFDPVYANWYTLNGNGDMTGTHWITESGFLETPILITNTGNVGTVRDAAW
                     QWMDQHHYYTPFFHDYWYAYPVVAETYDGIFNDINGQHVHRDDVFHALDSAGSGPVEE
                     GGVGGGTGMMCYGFKGGIGTASRVLDKRAGGYTVGVLVQCNHGSRYQLTIAGVPVGKA
                     LQDTLPFTMDKQPLGLASTPPREETGSIIVVLATDAPLLPGQLKRLAQRIPLGVSRTG
                     GIGGNGSGDIFIAFSTANPHAFSNTDERQVTTLPNDHINALFTATIEATEEAIINALF
                     AGKTLTGINGNTVYGLPKEAVIHLLRQYNRIPTK"
     gene            complement(2787..>4052)
                     /locus_tag="VKQ52_15835"
     CDS             complement(2787..>4052)
                     /locus_tag="VKQ52_15835"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ88727.1"
                     /translation="APIAVQRAEGVYLYDYDGKRYLDFSSGLMNVNIGHGNQRVTEAV
                     VRQMQEVSYVTPSCVTRARGELGRKLAEITPGGLQKTLFTVCGATAIENAIKLARLYT
                     GRHKIIARYRAFHGSSYGAMTAGGDPRKLPADAQQAPNFIHVEDPYCYRCPWSQEITT
                     CHRECVSHIERVIEFEGPETIAAILMEGESGSSGCIKYPPDYLQKVRALCDKYGILLI
                     ADEVMSGFGRTGKWFASELHGVVPDMIATAKGITAGYLPLGALIVTDKIANHFNDRVL
                     WLGLTYSAHPVSCAAGLEVLKIYEDENLIENAAAMGRYVDEQVARLREEHPCIGDWRN
                     TGLLGCLEIVKNRRTKEPMAPFNAKPDEMTIMNQVAARLKQLGMYTFVRWNYVFIAPP
                     LCITREQVDEGLAMISEALTIADAHVTKD"
BASE COUNT          737 a         1054 c         1457 g          804 t
ORIGIN      
        1 cttgttaaaa tgggtttcgg tgatgggttg acgatcggcc cgcatcagca ggccatagca
       61 gccgccggcg atggcgaagc cgacaaacca ggcatagctg tacaggtgaa cgatccaggc
      121 ggggaagctc ccgggaccgg cggcgcccac ggtcaccagg aaacccggga cattgggcag
      181 gatgccgagc agcaggcaga tcaccgcctt gcggttgatg cccgcggaaa agcggtagat
      241 gccgtttgga tcatagaggt cttttaccga gagctgttgc cgccggataa aataataatc
      301 gacgatcaaa attccgccga cggggccgag caagctggag taacccacga gccaggtgaa
      361 gatatagccg gtggggtcgg agaccagctt ccaggggaag atgaggatgc ccgcgatgcc
      421 ggtgatgaag ccgccggtgc ggaaattgat ctttgccggg gcgagattgg cgaagtcgtt
      481 ggcggggctg acgatattgg cggcgatgtt ggtggcgagg gtcgagatgg ccacggcgat
      541 catggcgatg ctgacgagga tcttgctgtc gaagcggccg gccagcgcca cggggtccca
      601 gatggttgtc ccgtagatga tcgtcgttgc ggaggtgacg accacgccga taaaggcgaa
      661 gagggtcatc gaggtcggca ggccaagggc ctggcctttc acctgggctt tctggctgac
      721 ggcatagcgg gtaaagtcgg gaatatttag ggaaagcgtc gcccagaagc cgaccatgcc
      781 ggtgaggccg gggataaaga aggcccagaa ggcggcgctg tccgggaatt tggccggctg
      841 ggtcaggatc gggccgaggc cgccgtgtac atggctgacg gcccagtaca aaagggcaag
      901 ggcggccagc ggcaggaaga aggccttaaa gacgaggagc ttccggatgc tgtcgacgcc
      961 cagccagacg acgtacatgt tcaggagcca gaacaggaga aagcagatgg cgggacccgt
     1021 ggcgagtcct agtgcggccg gtaactgcgg aagccggtcg agggcgggca cccagagatg
     1081 cagcatctgg taaagggcgt tgccgccgat ccaggtttgg atgccgaacc agccgcaggc
     1141 gacgatggcg cgaagcaggg cggggatatt ggcgcccttt gtcccgaaag aggcccgggc
     1201 gaaaaccggg aaggggatgc cgtactttgc gccggcgcgg ccgttcagga tcatggggat
     1261 cagcacgatg ctgttgccga ggaagatggt ggcgatcgcc tgccaccagt tcatcccgcc
     1321 gccgatgagg ctgctggcca gcatataggt cgggatacag aggctcatgc taatccagag
     1381 ggctgcatag ttccaggtgt tccaggttcg ttgctcccgg ccaatagggg cgaggtcttc
     1441 gctatagagc gaagtgggag agtggtccgg tagtttttct gcggccatag gcgatgattc
     1501 attactattt tgtcggaatg cggttgtatt gtcggaggag gtggatgacg gcttctttcg
     1561 gcaggccata gacggtgttc ccgttgatac cggtcagcgt cttgccggcg aagagcgcgt
     1621 tgatgatcgc ttcttcggtg gcttcgatcg ttgccgtgaa cagcgcgttg atatggtcgt
     1681 tgggcagggt ggtcacctgc cgttcgtcgg tattgctgaa ggcatggggg ttggcggtgg
     1741 agaaagcgat gaagatatcg ccggagccgt tgccgccgat gccgccggta cggctaacgc
     1801 caaggggtat ccgctgggcc aggcgtttca gctggccggg caggagcggg gcgtcggtgg
     1861 cgagtacgac gatgatggac cctgtctctt cgcgtggcgg ggtcgaagcc agtcccagcg
     1921 gttgtttatc catcgtaaag ggcagggtat cctgcagtgc cttgccgacg ggtaccccgg
     1981 cgatggtgag ctggtagcgg ctgccgtggt tgcactggac gaggacgccg acggtatagc
     2041 cgcccgcccg tttgtcgagt acccgggagg ccgtgccgat accgcccttg aagccatagc
     2101 acatcatgcc ggtgccgccg ccgacgccgc cctcctcgac gggcccggag ccggcgctgt
     2161 cgagggcgtg gaagacgtcg tccctgtgga cgtgctggcc gttgatatcg ttgaagatgc
     2221 cgtcataggt ctcggctact accgggtagg cgtaccagta gtcgtggaag aagggggtgt
     2281 agtagtgatg ctggtccatc cattgccagg cggcgtcgcg gacggtgccg acgttgccgg
     2341 tgttggtgat gaggatgggg gtctcgagga acccggattc ggtgatccag tgggtgccgg
     2401 tcatgtcgcc gttgccgttg agggtatacc agttggcata gacgggatcg aattccttac
     2461 cccggggcag gatcgccgtg acgccggtac ggatggggcc cttgcctttt acgaggggtc
     2521 cgtccccgct gatgagggtg gtctgaccga cggtgacgcc ttttacgtcg gtgatggcgt
     2581 tgagggggcc gggggcgcca tcgaagggga tgccgtagtc gcgggcacgc ttgatcggag
     2641 ccgaaggact tgtggcctgc tttggctgtc ctgccgtggc ggcggggatg tcaccggcgg
     2701 tggcagcggg ttgggcgttc gccgctgttt gaaaaagcag gagccagccg cagcagagcg
     2761 gtaagatagt tgatcgtttc agcatgttag tctttagtta catgggcatc tgcaatcgtc
     2821 agggcttcgc tgatcatggc caggccttcg tccacctgtt cgcgggtgat gcacaggggt
     2881 ggcgcgataa agacatagtt ccaccggaca aaggtgtaca tccccagctg cttcaggcgg
     2941 gcggccacct ggttcatgat ggtcatctca tccggctttg cgttgaaggg ggccatgggt
     3001 tcttttgtgc ggcggttttt aacgatctcc aggcagccca ggagaccggt gttgcgccag
     3061 tcgccgatac agggatgttc ttcccggagg cgggctacct gttcgtcgac atagcggccc
     3121 atggcggcgg cgttttcgat gaggttctcg tcttcgtaga tcttcagcac ttcgaggccg
     3181 gcggcgcagg acaccggatg cgcggagtag gtgaggccca gccagaggac gcggtcgttg
     3241 aaatggttgg cgatcttgtc ggtgacgatc aacgcgccca ggggcaggta cccggcggtg
     3301 atcccctttg ccgtggcgat catatcggga acgacgccat gcagctcgct ggcaaaccat
     3361 ttgcccgttc ggccgaagcc gctcattact tcgtcggcga tgagcaggat gccatatttg
     3421 tcgcagaggg cccgcacctt ctgcaggtag tcgggtgggt atttgataca accggaggaa
     3481 ccgctctcac cttccatcag gatggcggcg atggtctccg ggccttcgaa ttcgatgacc
     3541 ctttcgatat gggatacgca ttcccggtgg caggtggtga tctcctggct ccaggggcag
     3601 cgatagcaat agggatcttc cacatggatg aaattgggcg cctgctgggc gtcggcaggc
     3661 agtttgcggg ggtcgccgcc ggcggtcatg gcgccatagg aactgccatg aaaggcgcgg
     3721 tagcgggcga tgatcttgtg gcggccggtg tagagccggg ccagcttgat ggcgttttcg
     3781 atggcggtgg cgccgcacac cgtgaacagg gtcttttgca gcccgccagg ggttatctcc
     3841 gcgagcttcc tgcccaattc cccccgggcg cgggtgacgc aggatggcgt gacatagctt
     3901 acctcctgca tctggcggac gacggcttcg gtgacgcgtt ggttgccgtg accgatgttg
     3961 acgttcatca gcccggaaga aaagtcgagg tatcgctttc catcatagtc gtagagatag
     4021 acgccttcgg cgcgctggac ggcgatgggg gc
//
LOCUS       DATGFG010000841         7064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_42390, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000841 DATGFG010000000
VERSION     DATGFG010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42390"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VKQ52_15840"
     CDS             <1..632
                     /locus_tag="VKQ52_15840"
                     /inference="COORDINATES: protein motif:HMM:NF028180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88728.1"
                     /translation="PHGYEGQGPEHSSARMERFLQLCAENNIVVTDITTSANLFHAFR
                     RQLTWPFRKPLINFSPKANLRHPGTYSAMGEFTSGGFKEVIDDAFVGNAHDVKKVLFC
                     SGKVYFDLAERQQKDNRKDVAIVRLEQLYPLPVQQLENLYRKYNQAATWFWVQEEPLN
                     MGAASFLQMNLKSINYGVISRQPSAATATGYAKVHAQEQAEIIETAFSI"
     gene            672..1043
                     /locus_tag="VKQ52_15845"
     CDS             672..1043
                     /locus_tag="VKQ52_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HLZ88729.1"
                     /translation="MIDIKVPAVGESISEVTLVKWLKKEGQYVERDEVIAELESEKAT
                     FEVNAEKAGTLKPVGKEGETLKIGDVVAQIDETAARPEGQSAAATQAAANGAGTQTAQ
                     PATATAAAATATAAAPAAQGP"
     gene            1061..1942
                     /gene="odhB"
                     /locus_tag="VKQ52_15850"
     CDS             1061..1942
                     /gene="odhB"
                     /locus_tag="VKQ52_15850"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR01347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HLZ88730.1"
                     /translation="MRATPVAAAIITDKKVDAGSIKPTGTGGKILKNDVLEALAHPGR
                     TPGKELFTRADRREKMSNLRKTVSRRLVEAKNTTAMLTTFNEVDMSAVMALRAKYKDK
                     FKEKHGVNLGFMSFFTKATCYALQEWPAVNAYIDGDELVYHDYCDISIAVSAPKGLVV
                     PVIRNAESLSMADIEKKVVELATKARDNKLSMEEMQGGTFTITNGGVFGSLMSTPIIN
                     IPQSAILGMHKIEDRPVVVGGQIVVRPMMYLAVSYDHRIIDGRESVSFLVRIKELLEN
                     PDQLLFGKDPVKALLEL"
     gene            1975..3108
                     /locus_tag="VKQ52_15855"
     CDS             1975..3108
                     /locus_tag="VKQ52_15855"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88731.1"
                     /translation="MKFDNQLRYAVQIIDTYKGEVPLHAWLKDYFRANKQMGSRDRKQ
                     LSTLVYSFYRLGHSANHLPVKERILTGLFLCTEQPSELLAHFNPGYNDAITKPLPKKL
                     AVTGIRPTDIFPWKEELSPGIDHEGLCLSFLRQPDLFLRIRPGQESAVRAKLGDSDQA
                     EFILPATLRLPNGFKVEAHFTPDKEVVVQDYSSQRVAEFLRPAHSFWDACAASGGKSI
                     LAHDLYPEMDITVSDIRESILNNLRARFQAAGIKKYHSFLADLTRDDIVNLPIVHLVL
                     ADVPCTGSGTWGRTPEDLFFFNPTTIGRYQITQRKILTNIATHLAPGATLVYCTCSVF
                     KRENEEMVAFLRDDCHLQQDHTENLTGYLHRADTLFAARFTKR"
     gene            complement(3165..3845)
                     /locus_tag="VKQ52_15860"
     CDS             complement(3165..3845)
                     /locus_tag="VKQ52_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88732.1"
                     /translation="MTDYFLTLQVGKYVTYRLDSLNFYYYGQLDTVTSYLAKDSVEGS
                     FVDGSGNTAWNVTRYMSDTTGSNWYPGESYIVTPSQQRIEMTENNLRYIKLAFPLDQG
                     FSWSGNSYLPYAPYKDFFDYSDDSHLTLSSWNYTYQAMNKPYTVGNLHFDSTLTVLQE
                     NDSINVPITDPNSFASRTYWSETYARHIGLVYRHTEMWEYQPPTPDGTQSGYKIGFQM
                     TMRIVDHN"
     gene            complement(3983..5083)
                     /gene="mnmA"
                     /locus_tag="VKQ52_15865"
     CDS             complement(3983..5083)
                     /gene="mnmA"
                     /locus_tag="VKQ52_15865"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HLZ88733.1"
                     /translation="MSRKGKVLVAMSGGIDSTITALMLHDQGYEVVGITMKTWDYASA
                     GASKKETGCCNLDSFNDARMAAVQHGFPHFVLDIREEFGDFVIENFVDEYLAGRTPNP
                     CVLCNTHIKWRALLKRADAMDCEFIATGHYGMIRQHTNGRYTIARGVDETKDQSYVLW
                     GLQQDLLKRTLLPLGPYRKTEIRQMALDYGYPELAKKSESYEICFVPDNDYRGFLKRR
                     VDGLEERVAGGNFVDKHGNILGKHKGYPFYTIGQRKGLDVTFGRPVYVTAIEPDSNTV
                     VLGEEEDLDRQEMFVGKINWMKYEGVSAGMESMTRIRYKDKGTLSNLYPDGDRVRVVF
                     YEKAKSIAPGQSAVFYEGDEVIGGGIIQRAGV"
     gene            complement(5224..5706)
                     /locus_tag="VKQ52_15870"
     CDS             complement(5224..5706)
                     /locus_tag="VKQ52_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88734.1"
                     /translation="MNKLIGTVLAGLFCLVQHGVQAQAPGNASLLNVHSIGTDVAAVK
                     ASRDFWKQVGDQKNEQWYKETTGYSAEYTDGPVKALYRYDKNGKWLYSILTYGENRLP
                     EEVRHLVRSTYYDFGIQWVKEVGDAQSTVYVVHIENDTAWKEVAVQDGEMRVLKEFCK
                     "
     gene            complement(5851..6597)
                     /locus_tag="VKQ52_15875"
     CDS             complement(5851..6597)
                     /locus_tag="VKQ52_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ88735.1"
                     /translation="MIKAIIVDDEPYCCEALSILLDRYCPQVEVAGVFHSGAQALEPI
                     RELKPQLLFLDIEMPRMNGFELLEKVKSLNFELIFTTSYDQYAIKAIRFSALDYLLKP
                     IDREELQLSIQKVEQRLQAPLPQQFDLLFQRLQNPLYTRIAIPTMEGLQMVAVDSIVH
                     CVSDSNYTVLTLKTRQKVVASRTLKEIEELLEDYGFLRVHHSNLVNLNEVCKYIKGEG
                     GYLVMSDGSSINVSRSRKEGLLKKLQPNRS"
     gene            complement(6609..>7064)
                     /locus_tag="VKQ52_15880"
     CDS             complement(6609..>7064)
                     /locus_tag="VKQ52_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88736.1"
                     /translation="LDMERLRFKDAFDYRIDVEEDLDTEDIRIPPLLVQPFVENAVWH
                     GLMNKEERGFLSIRIRVDGDVLTCIVRDNGVGRKRAGLLKSKSVEKHKSMGLQITAER
                     MALLTGPGESQPFFHIEDLYDEAGFPTGTQVTLTIKINQPATEPVKPII"
BASE COUNT         1670 a         2254 c         1647 g         1493 t
ORIGIN      
        1 tgccccatgg ctacgaaggc cagggccccg agcacagcag cgcccgcatg gaacgcttcc
       61 tgcaattgtg cgcggaaaac aatatcgtcg tcaccgacat aacgacttcc gccaatctct
      121 tccatgcctt ccgccgtcag ctgacctggc ccttccgcaa accgctgatc aacttctcgc
      181 caaaggcaaa cctccgtcat ccgggaacct actcggcgat gggggaattc accagcggcg
      241 gcttcaaaga agtgatcgac gatgcctttg tcggcaacgc gcacgacgta aagaaggttc
      301 tcttctgcag cggtaaggtc tacttcgatc tcgccgaacg ccagcagaaa gacaaccgca
      361 aggacgtcgc catcgtccgg ctcgaacaac tctacccgct gcccgtccag caactggaaa
      421 atctgtacag gaaatacaac caggcggcta cctggttctg ggtccaggaa gaacccctca
      481 acatgggcgc cgcttccttc ctccagatga acctgaaatc gatcaactat ggggtcatca
      541 gccgccagcc cagcgctgcc acggctaccg gttatgccaa ggttcacgca caggaacagg
      601 ccgagatcat cgaaacagca ttctccattt aatagattgt agcgtacatt tgcaaaccaa
      661 aggcacaaat aatgattgat ataaaagttc cggcagtagg cgagtccatc agtgaagtaa
      721 ccctggtgaa atggctcaaa aaggaaggtc aatatgtcga acgagatgaa gtgattgcag
      781 aactggaaag cgaaaaagca acgttcgagg tcaacgccga aaaagccggc acccttaaac
      841 ccgtcggaaa agaaggcgag accctcaaga tcggcgacgt ggtcgcccag atcgatgaga
      901 cggccgcccg ccccgaaggt caaagcgccg ccgccacaca ggccgccgcc aacggcgccg
      961 ggacccaaac ggcacaacct gcgacggcaa cagccgccgc cgctacagca accgcggccg
     1021 ctccggctgc ccaaggcccg taacatcgat ccccaacgat atcagggcga cccccgtcgc
     1081 cgcggccatc atcaccgata aaaaggtgga tgccggctcg atcaaaccca ccggcaccgg
     1141 cggcaagatc ctcaagaacg atgtcctcga agcgctggca catcccggtc gtacgcccgg
     1201 taaagaactc ttcacccgcg ccgatcgacg ggagaaaatg tccaacctgc gcaagaccgt
     1261 atcccgccgc ctcgtagagg ctaagaatac gacggctatg ctcaccactt ttaatgaggt
     1321 ggatatgagc gccgtcatgg ccctccgtgc aaaatacaag gacaagttca aggaaaaaca
     1381 tggcgtcaac ctgggcttca tgagcttctt tacaaaagct acctgctacg ccctgcagga
     1441 atggcccgcc gtgaacgcct acatcgacgg cgacgaactc gtctatcacg actattgcga
     1501 tatctccatc gccgtatccg cgccaaaagg tctcgtcgtc ccggtgatcc gcaacgccga
     1561 aagcctcagt atggccgata tcgaaaagaa agttgtcgaa ctcgctacaa aggcccgcga
     1621 caacaagctc tccatggaag agatgcaggg cggaaccttc accattacca acggtggggt
     1681 ctttggcagc ctgatgtcca cccccatcat caacattccc cagtccgcga tccttggcat
     1741 gcacaagatc gaagatcgcc ccgtcgtcgt aggcggccag atcgtcgtcc gcccgatgat
     1801 gtacctggcg gtcagctacg atcaccgtat catcgatggc cgcgaatcgg tcagcttcct
     1861 ggtccggata aaagaattac tggagaaccc cgatcaactg ctgttcggca aggatcccgt
     1921 gaaagcattg ctggaattat aacccgggcc cgccgcggag ccctatgttc gctcatgaaa
     1981 ttcgacaacc aactgcggta cgcggtccag atcatcgaca cctataaggg cgaggttccg
     2041 ctgcatgcct ggctgaagga ttatttccgc gcgaacaaac aaatgggctc gcgcgatcgc
     2101 aaacagcttt ccaccctcgt ctacagtttc taccgcctcg gtcactccgc caaccacctg
     2161 cccgtaaaag agcgtatcct caccggcctc tttctttgta cggaacagcc cagtgagctt
     2221 ctcgcccatt tcaaccccgg ctataacgac gccatcacaa agcccctccc caaaaagctc
     2281 gccgtcaccg ggatccgccc caccgacatc ttcccctgga aggaagaact ctcccccggc
     2341 atcgaccacg aaggcctctg tctctccttc ctccgccagc ccgatctctt cctccgtatc
     2401 cgccccggcc aggaaagcgc tgttcgcgca aaactcggcg atagcgacca ggccgaattc
     2461 atcctgccgg caacccttcg gcttcccaat ggttttaagg tcgaagccca tttcacgccg
     2521 gacaaagagg tcgttgtgca ggactacagc tcccaacgcg tcgcggagtt tctccggccg
     2581 gcgcacagct tctgggacgc ctgcgccgcc agcggcggca agtccatcct ggcccacgat
     2641 ctctatccgg aaatggatat tacggtatcg gatatccgcg aatcgatcct gaacaatctc
     2701 cgcgcccgct tccaggccgc aggcataaaa aaataccatt ccttccttgc cgacctcacc
     2761 cgggacgata tcgtcaatct ccccatcgtc catctcgttc tcgccgacgt gccctgcacc
     2821 ggcagcggca cctggggccg gaccccggaa gacctcttct tcttcaaccc aacgacgatc
     2881 ggccgctacc agatcaccca acgaaagatc ctcacgaaca tcgccaccca tctcgctcca
     2941 ggcgccacgc tcgtctattg cacctgctcc gtctttaaaa gggaaaatga agaaatggtc
     3001 gccttcctcc gcgacgattg tcatctgcaa caagatcata ctgagaatct caccggttat
     3061 ctgcatcggg ccgacaccct cttcgcggcg cgctttacga aacgctgacc gcgctgccca
     3121 gccgcccgca ccgcccagcc gcccgcggca ccagctgcct gcgtctaatt atgatccacg
     3181 atccgcatcg tcatctggaa cccgatctta tacccgctct gtgtcccatc cggggtcggc
     3241 ggctgatatt cccacatctc cgtatggcga tataccagcc cgatatgcct ggcataggtc
     3301 tcgctccaat acgtgcggga agcaaacgag ttcggatcgg tgatcggcac attgatggaa
     3361 tcgttctcct gcagcaccgt taatgtgctg tcaaaatgca gattgccaac cgtatagggc
     3421 ttgttcatcg cctggtaggt atagttccag gaactcagcg tgaggtggga atcatcgctg
     3481 taatcgaaga agtccttata gggcgcatag gggagatagg agttgcccga ccagctaaag
     3541 ccctggtcca gggggaaggc cagtttgata taccgcaggt tgttctccgt catctcgatc
     3601 cgctgctgcg aaggcgtgac gatataggat tcgcccggat accagttgct cccggtcgta
     3661 tcgctcatat agcgcgttac attccaggcc gtattacccg aaccatcgac aaaactgcct
     3721 tccacggaat ccttcgccag gtaggaggtg acggtatcca gttgcccgta ataatagaag
     3781 tttaaggaat ccagccggta agtaacatat tttcccacct gtaaggtcag gaaatagtcc
     3841 gtcaaattgg ttgcgggata atgtatcgtt tttttgcagc tggctatgac gagcacaaaa
     3901 caaagcagta tgagtacaac cggacgaggc ttcatacctt ctgtaacggg aaaaggggca
     3961 atttattgcc ccttccggcc ggctaaaccc cggccctttg tattataccc ccaccgataa
     4021 cttcatcccc ctcgtaaaag accgcgctct gtcccggggc gatgcttttc gccttctcgt
     4081 aaaacaccac cctgacccgg tctccatcgg ggtaaagatt gctgagcgtc cccttatcct
     4141 tgtaccggat cctggtcatc gactccatac cggcagatac cccttcatac ttcatccagt
     4201 tgatcttccc gacgaacatc tcctgccggt cgagatcctc ctcctctcca aggacgaccg
     4261 tattcgagtc cggctcgatc gccgtcacat agaccggccg gccaaaagtc acatccagtc
     4321 ccttgcgctg cccgatcgta tagaaaggat aacccttatg cttcccaagg atattcccat
     4381 gcttgtcgac aaaattgcca ccggccaccc gctcttccag gccatccacc cggcgcttca
     4441 aaaaaccgcg gtagtcattg tccggtacaa aacagatctc ataactctca ctcttcttcg
     4501 ccagttcggg gtatccatag tcgagcgcca tctgccggat ctccgtctta cggtaaggcc
     4561 caagcggcag cagggtccgc ttcaacaggt cctgctgaag cccccacaat acatagctct
     4621 gatccttcgt ctcatccaca cccctggcga tagtatagcg gccattggta tgctgacgga
     4681 tcatcccata atgccccgtt gcaataaact cacaatccat ggcgtcggcc cgcttcaaca
     4741 gcgcgcgcca cttgatatgc gtattacaaa gaacacaggg attgggcgtc cgccccgcga
     4801 gatattcgtc gacaaaattc tcgatcacaa agtcgccaaa ctcttcgcgg atgtccagca
     4861 cgaaatgcgg gaacccatgc tgtaccgcgg ccatccgcgc atcattaaaa ctatccaggt
     4921 tacaacatcc ggtctctttt ttcgaagcgc ccgcactggc ataatcccag gtcttcatgg
     4981 tgatacccac cacttcatac ccctgatcat gcaacatcaa cgccgtaatg gtactatcga
     5041 taccaccact catcgctact aacacctttc ctttgcggct catagaattg cgcttttgat
     5101 ttttacctaa aactaaatcc aaagcgcaat tccgctaatg tcgggccttc ggccgacgga
     5161 cccgtggtcc gtcttgtttg cgcctttaaa aaattacaag ctgcaaattt acacctctcc
     5221 cggtcattta caaaactcct tcagcacccg catctcccca tcctgtacgg ccacctcctt
     5281 ccaggcggtg tcgttctcta tatgtaccac gtatacagtg ctttgcgcat cacccacctc
     5341 ctttacccac tggatgccga agtcataata cgtgctccgc acaaggtggc gcacctcttc
     5401 cggcaaccgg ttttcgccat acgtgaggat gctgtataac catttcccgt tcttgtcata
     5461 ccggtacaat gcctttaccg gtccatcagt atactccgcg gaataccccg tggtctcctt
     5521 ataccattgc tcgttcttct ggtcgccgac ctgcttccag aaatcccgcg acgccttcac
     5581 cgccgcaaca tccgttccga tggaatggac gttcaataaa gaggcattgc caggcgcctg
     5641 cgcctgcacg ccatgctgaa caaggcaaaa caaccctgcc agtactgttc cgattagctt
     5701 attcatacct tttgttttac tcgtgaaaga ataaaacaaa gctaatccgg catcgtcacc
     5761 ggcgccagcc aaaacccgta actggtcgca atgggaattc atcaacggaa atgggtgagc
     5821 agttggttat acggcaggaa ggcgtgaccg ctaggacctg ttgggttgta atttctttaa
     5881 caatccctcc ttacggctgc gggaaacatt gatagacgac ccgtccgaca tgacaagata
     5941 gccgccttcg ccctttatat acttgcaaac ttcattgaga ttgacaagat tggaatgatg
     6001 gacacgcagg aaaccataat cctccagcaa ttcctcgatc tccttcagcg tcctcgacgc
     6061 cacgaccttt tgccgggtct tcaaggtcag caccgtgtaa ttgctgtccg agacacaatg
     6121 gacgatactg tccaccgcca ccatttgcag cccttccatc gtcgggatcg cgatgcgggt
     6181 atacagagga ttctgcaatc gctgaaagag cagatcgaac tgctgcggca ggggcgcctg
     6241 caaccgctgt tccaccttct gaatggacaa ttgcagctct tcccggtcga tgggttttaa
     6301 aagataatcg agcgcgctga atcgtatcgc ctttatcgca tattgatcat agctcgtggt
     6361 aaagatcaac tcgaaattca ggctcttcac cttctccagc aattcaaacc cgttcatccg
     6421 cggcatctcg atgtcgagga aaagcagctg cggcttcagc tccctgatcg gctccagcgc
     6481 ctgcgccccc gaatgaaaca ccccggccac ctccacctgc ggacaatacc ggtccagcag
     6541 gatcgaaagg gcctcgcaac aatagggctc gtcatcaaca atgattgcct tgatcatcga
     6601 tcatggattt agataatagg ttttaccggt tccgtggccg gctgattgat ctttatcgtc
     6661 agggtcacct gtgtgccggt ggggaaaccc gcttcgtcat agaggtcctc gatgtggaaa
     6721 aaaggctgcg actcacccgg ccccgtcagc agcgccatcc gctcggccgt tatctgcaac
     6781 cccatcgact tatgcttctc gacggatttg cttttcagca atcccgcccg cttgcgcccg
     6841 acgccgttgt cccggacgat acaggtgagc acatcgccgt ccacccgtat ccggatcgac
     6901 aaaaagcccc gttcctcttt gttcatcaac ccatgccaga cggcattctc gacaaagggc
     6961 tgcaccagca gcggcggtat ccggatatcc tcggtatcga ggtcttcctc cacatcgatg
     7021 cggtaatcaa aagcatcctt gaaccgcaac cgctccatat ccag
//
LOCUS       DATGFG010000842         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_874103, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000842 DATGFG010000000
VERSION     DATGFG010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874103"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..819)
                     /locus_tag="VKQ52_15885"
     CDS             complement(304..819)
                     /locus_tag="VKQ52_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88737.1"
                     /translation="MDQTPTNTAIQQDPATEKRTFILEVIQPGLAGLMDGSVSSLAPL
                     FAAALTTRSSHTAFLVGMATAVGAGISMAFSEALSDDGKLSGRGNPILRGGVCGAMTF
                     VGAAGHALPFLVPAFNVAMTVAIAIVIVELFVIAWVRHRYMETPLGKAAIQIIIGGVI
                     VFIAGILIGKS"
     gene            complement(854..1990)
                     /locus_tag="VKQ52_15890"
     CDS             complement(854..1990)
                     /locus_tag="VKQ52_15890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ88738.1"
                     /translation="MKRKLFWAVLFIIPIGILFPSCGNPAPAATRPAIDSATDPAVAI
                     QTISLSKGRLTSTITLPGQLLPFQSVDLYAKVNSFVKEMFVDVGSRVHKGQLLATLEA
                     PELQSATDEAASLLHTREAAFRASKATYQRLYATSKIPGTVSPNELELANAKMSSDSA
                     NFAAARSHYQQSADLLGYLEVRAPFDGVISSRNIYPGAYAGPAGKGSFIPLMTLQQQR
                     RLRLVVAVPEAATAYFRDRDTVHFTVQTLPGRDFTATVTRMAGALDLQLRTEQLQMDV
                     ANDNGLLLPGMFAQVTLDCTNTDKQFIVPTAAVTANSKRIFVIRVTGGKTEWVTVQKG
                     RESSGKVEIYGDLREGDQLVATATDELKSGTTVRVNQAAEPIDN"
BASE COUNT          387 a          481 c          731 g          451 t
ORIGIN      
        1 aggttgcgaa tggtgcgaat gatcatggcc atgcattgat ttgaagtgag accaaatgta
       61 gaagccggga aaggactaaa tcatgatgta aatcgcatcg ctgcctgatc agaatcatgt
      121 tgacccctgt tttgcatgat cctggtcgcg tctcgttgaa ataggctcca gatcataagc
      181 gcgggttgcg ggccggtgta atcttgtgta aaatttacta aaaagcgcaa atatgaagaa
      241 atatctttta ctatccgccc tcgtgggcag atctgtgcct gcggaaacgc tgagcggggg
      301 gcgttagctt ttcccgatga ggatgccggc gatgaagacg atgacgccgc cgatgatgat
      361 ctggatggcg gccttgccga gaggtgtctc catgtagcgg tggcggaccc aggcgatgac
      421 gaagagttcg acgatgacga tggcgatggc gacggtcatg gccacattga atgccgggac
      481 gagaaagggc agggcgtgac cggcggcgcc gacgaaggtc atggccccgc aaacgccgcc
      541 acgcaggatg gggttaccgc ggccgctgag ctttccgtcg tcggagaggg cttcggagaa
      601 ggccatgctg atgcctgcgc cgacggcggt ggccatgcct acgaggaagg cggtatggct
      661 gctccgggtg gtgagggccg cggcgaagag cggcgcgagg gaagagacgg agccgtccat
      721 gagaccggcg agcccgggct ggatcacctc caggataaag gtgcgttttt cggtggcagg
      781 gtcctgttga atggcggtat ttgttggtgt ctgatccatg tcagcgattt tgtgtgaagt
      841 tacgggaata gggttaattg tcgatgggtt cggcggcctg gttgacacgg acggttgtgc
      901 cgcttttcag ttcgtcggtg gcggtggcga cgagttggtc gccttccctg agatcgccat
      961 atatttctac ttttccactg gattccctcc ctttttgtac ggtgacccat tcggtctttc
     1021 cgcccgtgac gcggatgacg aagatgcgtt tgctattcgc cgtcaccgcg gcggtgggta
     1081 cgatgaactg cttgtcggta ttggtgcagt cgagcgtcac ctgggcgaac attccgggca
     1141 ggaggaggcc gttgtcattg gcgacgtcca tctgaagttg ttcggtacgc aactggaggt
     1201 cgagggcgcc ggccatgcgg gtgaccgtcg cggtgaagtc gcggccgggc agggtctgga
     1261 cggtaaaatg aacggtgtcc cggtcgcgga aataggccgt tgccgcctcg ggaacggcga
     1321 cgaccaggcg aaggcgacgc tgctgctgga gggtcatcag gggtatgaag gaacccttgc
     1381 ccgcggggcc ggcgtaagcg ccgggataga tgttacggct gctgatgacc ccgtcgaagg
     1441 gagcccgcac ttccaggtaa cccaggagat cggcggactg ttggtagtgg gatcgggcgg
     1501 cggcgaaatt ggcgctatcg ctgctcatct ttgcgtttgc cagttccagc tcgttggggg
     1561 atacggtccc gggtattttg ctggtggcat agagccgctg gtaggtggct ttggaggcgc
     1621 ggaaagcggc ttcgcgagta tggagcaggg acgctgcttc atcggtggcg gactggagtt
     1681 ccggcgcttc gagggtagcg aggagttgtc ccttgtgcac gcggctgccg acatcgacaa
     1741 acatttcttt tacgaagctg ttgacctttg catagaggtc cacggactgg aagggcagca
     1801 gttgccccgg cagggtaatg gtggaggtga gccggccctt gctcagggaa atggtttgta
     1861 tggccacggc gggatcggtg gcagaatcga tggcgggtct ggtggcggcc ggggccgggt
     1921 tgccgcagga cgggaagagg atgcctatcg ggatgataaa gaggacggcc caaaatagtt
     1981 tgcgtttcat tctttggtat ttattggttc gtaatacagg ctttgtacat cgtcggggtc
     2041 gagtgagacg
//
LOCUS       DATGFG010000843         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_196692, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000843 DATGFG010000000
VERSION     DATGFG010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196692"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VKQ52_15895"
     CDS             <1..209
                     /locus_tag="VKQ52_15895"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HLZ88739.1"
                     /translation="YVIGPLAASEVSGYLPGGDRYAVIETFNRLFVPAEADVETEIEI
                     DRTTLEMRLEVGMEPVLGYSSILT"
     gene            248..1120
                     /locus_tag="VKQ52_15900"
     CDS             248..1120
                     /locus_tag="VKQ52_15900"
                     /inference="COORDINATES: protein motif:HMM:NF036941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssN family type VI secretion system protein"
                     /protein_id="HLZ88740.1"
                     /translation="MTAERWLYPEMGRWMMLLGLGTIGLSALMGILISRVRGGFKAFS
                     KRTILYTLSYVLLFALSGFCIALPFPQYLYAFIFFQVLYLGMGVLHLFTGARWLKWMG
                     AGALWPEMLHLVVMMLLGAVGMIMTYRIVNREGAEIAMSTSTLFFIVPYFIHKAYLRS
                     VAIPPKVYKQWYYPVQRREPELEESKLRNLLVISFEFQKQMNDRYFTNFRAKAPVDME
                     FGQLFYYFINDYNDRHGGSAIRYSDNHGDLHGWIFYRKPKWYHLSTKYIDTEKTVFIN
                     NIKENDVIICNRVS"
     gene            1152..>2064
                     /gene="tssK"
                     /locus_tag="VKQ52_15905"
     CDS             1152..>2064
                     /gene="tssK"
                     /locus_tag="VKQ52_15905"
                     /inference="COORDINATES: protein motif:HMM:NF017723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HLZ88741.1"
                     /translation="MKINKDHFRADQQAAIWQQAQHSRAGLGAVNYGLFPSGEGQLGQ
                     PVVVTVDNQQRVMIRLIACQAVTAGGHLIYVREGSRAGGDALTATIPGLSVPYAQLAA
                     ADAEYYVMLCVNPYNRVPAGQPDMEESQLRPPFTAPAYRLLLTDCRESRREAPGEFFL
                     PLGRIRVSEGKVLPDEAYIPPCTCIAAHPALSEWHAELEQFYGAMESYVLRIQQKIVQ
                     KGQQNDLAGLVADLCNVMAPFMSADYQRVLMEYAYQVPVRAIAGVAGLARLMKNRLDV
                     YTGPMKDELLNYFAEWCSVQQGEWESVL"
BASE COUNT          415 a          500 c          675 g          474 t
ORIGIN      
        1 agtatgtgat cggtccgctg gccgcttccg aggtgagcgg ttacctgccg ggcggagacc
       61 ggtatgccgt gatcgagacc tttaaccggc tgttcgttcc tgccgaggcc gatgtcgaga
      121 cggagatcga gatcgacagg accacgctgg agatgcgact ggaggtgggg atggaacccg
      181 tgctgggcta ttcatcgata ttgacataat tacatcattc attcatacac ctgtaataga
      241 aaccgatatg acagcagaaa gatggttata tcccgaaatg gggcgctgga tgatgctgtt
      301 gggactgggg acgatcgggc tttccgccct gatgggcatc ctgatcagcc gggtcagggg
      361 tggcttcaag gcctttagca agcggacgat cctgtacacg ctttcctatg tcctgttgtt
      421 tgcattgagt ggtttttgca tcgccctacc cttccctcaa tacctgtatg ccttcatctt
      481 tttccaggtg ctttacctgg ggatgggggt gctgcatctt tttaccggtg cccgatggct
      541 gaagtggatg ggcgcggggg cattgtggcc ggagatgtta cacctggtgg tcatgatgtt
      601 gctgggggcg gtggggatga tcatgacgta caggatcgtc aaccgggagg gagcggagat
      661 cgcgatgtcg acgtcgacct tgttctttat cgttccgtat ttcatccaca aggcctatct
      721 gcggagcgta gccatcccac cgaaggtgta caagcaatgg tattatcccg tgcagcggcg
      781 ggaaccggag ctggaagaat cgaagctgcg gaacctgctg gtgatcagtt tcgagttcca
      841 gaagcagatg aacgaccggt atttcacgaa tttccgcgcc aaggcgccgg tggatatgga
      901 gttcgggcag ctgttctatt atttcatcaa cgattataac gatcgccatg gcgggtcggc
      961 tatccggtat tccgataacc acggggatct gcatggctgg atcttttacc ggaagcccaa
     1021 gtggtatcat ctgtccacca aatatatcga tacggaaaaa accgtcttca tcaataatat
     1081 aaaagaaaat gatgtcatca tctgcaatag ggtctcctga gatctttcta cctgtcaatt
     1141 gggccgatgg aatgaagatc aataaggatc atttccgggc cgatcagcag gctgccatct
     1201 ggcaacaggc gcagcactcg agggccgggc ttggcgcggt gaattatgga ttgttccctt
     1261 cgggggaagg acagttgggg cagccggtgg tcgtgacggt ggataaccag caacgggtga
     1321 tgatccggct gatcgcctgt caggcagtga cggccggagg ccacctgatc tatgtccgtg
     1381 agggttcgcg ggccggcggg gatgcgctga cggcgacgat cccggggctt agcgttccct
     1441 atgcgcaact ggccgcggcc gatgcggagt attatgtcat gctttgtgtg aatccttata
     1501 accgggtgcc cgcggggcag cccgatatgg aggagagtca gctgcggccg ccgttcacgg
     1561 cccccgccta ccggttacta ctgaccgatt gccgggagag ccggcgggag gccccggggg
     1621 aattctttct tcctcttggc cggatcaggg tcagcgaggg gaaggtgttg ccggatgagg
     1681 cctatatccc accctgtacc tgtatcgcgg cgcatcccgc gctgagcgag tggcatgcgg
     1741 agctggagca gttctatggg gcgatggagt cctatgtgct gcgcattcaa cagaagatcg
     1801 tacagaaggg gcagcagaat gacctggcgg gcctggtggc cgacctgtgc aatgtgatgg
     1861 cgccgttcat gtcggccgat taccagcggg tgttgatgga gtatgcctac caggtgcccg
     1921 tacgggcgat cgcgggagtg gcggggctgg cgaggctgat gaagaaccgg ctggatgtat
     1981 ataccgggcc gatgaaggat gaactgttga actatttcgc cgagtggtgt tcggtacagc
     2041 agggggaatg ggagtcggtg ttgc
//
LOCUS       DATGFG010000844         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_277942, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000844 DATGFG010000000
VERSION     DATGFG010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277942"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VKQ52_15910"
     CDS             complement(<1..513)
                     /locus_tag="VKQ52_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ88742.1"
                     /translation="MILIVDDKPENIFSLKTILELHSFPTDTALSGEEALKKILRQSY
                     ALIILDVQMPGMDGFEVAEAISGYSKARDIPIIFLSAANTDKKFITKGYSSGGMDYIT
                     KPIDPDILLLKVKTFYRLYEQNRELNRIQTSLRSEIEFRKKVEGELQERAGELQSTLE
                     SIPQIAFTTGA"
     gene            633..1388
                     /locus_tag="VKQ52_15915"
     CDS             633..1388
                     /locus_tag="VKQ52_15915"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88743.1"
                     /translation="MQTRTIDFFWNAGDLTAFMLRFFKEVVKPPYEWREVFYQCYLVG
                     YKSTFLIGLTAFIMGAVLAIQTRPSMAAFGAIAMIPGMVSVSVVREIGPVITAIICCG
                     KVGSRMGAELGSMRVTEQIAAMEVSAINPFHFLVVSRTVATTLMVPLLTIIADLVAFF
                     GAYLAMNIHDATTVRHFFVAAMSHLGFIDIFPAVIKTFFFGYVIGIIGCYKGYYARQG
                     TESVGIAANSAVVAGSLAVFIIDMIAAQITDLL"
     gene            1396..2256
                     /locus_tag="VKQ52_15920"
     CDS             1396..2256
                     /locus_tag="VKQ52_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ88744.1"
                     /translation="MKKESIEETSPTIEEVQQPPLPDPLPTPDTNVQPQEEETPPGDE
                     QPVIIIRGLTKAFDEHEVLRGIDLHVNKGENLVVLGKSGTGKSVLIKCLVRLEWPDSG
                     KITICGQDLDTLNYTEMNALRLRIGFLFQNAALYDSMTVRENLAFPLRQHKKTMTKER
                     KEALIMEMLDNVGLVDSIDQLPSKLSGGQSKRIGLARTLILRPEIMLYDEPTTGLDTG
                     TAKEISELICKMKKKYNISSITITHEMPCAKMTADRIILLKDGIVYAEGSYDQLETSK
                     DEWIRSFFDF"
BASE COUNT          546 a          733 c          509 g          473 t
ORIGIN      
        1 cgcccccgta gtaaacgcaa tctgcgggat ggactccagc gtcgactgaa gttcccccgc
       61 ccgctcctgc aactccccct ccaccttctt ccggaactca atctcgctgc gcagactggt
      121 ctgtatccgg ttcaattccc ggttttgttc atacagccgg taaaaggtct tcaccttcag
      181 caacaggata tccggatcta tcggtttggt gatataatcc ataccccctg aactgtaacc
      241 ctttgtaatg aatttcttat cggtattggc ggcggaaagg aaaatgatcg ggatatccct
      301 cgccttgcta tagccgctga tcgcctcggc cacctcgaaa ccatccatcc ccggcatctg
      361 gacatccagg atgatcagcg catacgactg ccggaggatc ttcttcaaag cttcctctcc
      421 gctcaatgcc gtatcggtgg gaaaagaatg caattccagg atggtcttga gggaaaagat
      481 attctcgggc ttatcatcga cgatcaggat catacccaaa tgtaagcgat tccccgctcg
      541 tcgctgatag ccaggctgtg gaactatttc tacaattttg gaaaaacaac ggcgtgccac
      601 ttttggacta ataattgaat ccattacggt atatgcagac aagaacaatc gacttcttct
      661 ggaatgccgg cgacctcacc gcctttatgc tgcgcttctt caaagaggtg gtcaaaccac
      721 cctatgaatg gcgggaggtc ttctatcaat gctacctcgt cggttataaa tccaccttcc
      781 tcatcggcct cacagccttt atcatgggcg ccgtactcgc catccagacc cggccctcca
      841 tggcggcctt cggggccatt gccatgatcc ccggcatggt ctccgtctcc gtcgtcaggg
      901 agatcggccc cgtcatcacg gccatcatct gctgcggaaa agtaggctcg cggatgggcg
      961 ccgaactcgg ctcgatgcgc gtgaccgaac agatcgccgc catggaggtc tcggccatca
     1021 accccttcca cttcctggtg gtctcccgca ccgtcgccac aaccctcatg gttcccctgc
     1081 tgaccatcat cgccgacctc gtcgccttct ttggggccta cctggcaatg aatatccatg
     1141 atgccaccac cgtccggcac ttcttcgtgg cggccatgag ccacctggga ttcatcgata
     1201 tcttccccgc cgtgataaaa accttcttct tcggctatgt catcggtatc atcggctgct
     1261 ataaggggta ttacgcccgg cagggaacgg aaagcgtcgg catcgccgca aacagcgcgg
     1321 tcgtggccgg ctccctggcc gtattcatca tcgacatgat cgccgcccag atcactgacc
     1381 tgttatagat aaaatatgaa aaaagagagc atagaagaga ccagcccaac catcgaagag
     1441 gtgcagcagc cccctctccc cgaccccctg cccactcccg acaccaacgt ccaaccccag
     1501 gaagaagaaa caccccccgg agacgaacaa cccgtcatca tcatccgcgg tctgacaaaa
     1561 gcctttgacg aacatgaggt cctccgcggt atcgatctcc acgtcaacaa aggcgaaaac
     1621 ctggtcgttc tcggtaaatc cggcacgggc aaatccgtcc tcatcaaatg tctcgtccgg
     1681 ctggaatggc ctgacagcgg aaagatcacc atctgcggcc aggacctcga caccctcaac
     1741 tacacggaga tgaacgccct ccgcctccgc atcggcttcc tcttccagaa cgccgccctc
     1801 tacgactcga tgaccgtccg cgaaaacctc gccttccccc tgcggcagca taaaaaaaca
     1861 atgacaaagg aacggaagga agcactcatc atggagatgc tcgacaacgt aggcctcgtc
     1921 gactccatcg atcaattacc atccaaactc tccggcggcc agagcaaacg catcggcctg
     1981 gcccgcaccc tcatccttcg cccggagatc atgctctacg atgaacccac caccggcctc
     2041 gacaccggca ccgccaaaga gataagcgaa ctcatttgca aaatgaaaaa gaaatacaac
     2101 atctcctcca tcaccatcac ccacgaaatg ccctgcgcaa agatgacggc cgatcgcatc
     2161 atcctcctga aggacggcat cgtctacgcg gaaggaagct acgatcaact ggaaacctcc
     2221 aaagatgaat ggatcagatc cttttttgat ttttaaccct a
//
LOCUS       DATGFG010000845         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1069106, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000845 DATGFG010000000
VERSION     DATGFG010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069106"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VKQ52_15925"
     CDS             <1..1160
                     /locus_tag="VKQ52_15925"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLZ88745.1"
                     /translation="GDHQSKLTLMSESLRNDGRIWVPKKKDDPRKPNEIPAEERDYYL
                     ERRYPAFGNLVPRDVASRAAKERCDAGYGVGTSKQAVYLDFASAIERYGKTECRKHGI
                     DNPTAEDIRTHGVDVVKEKYGNLFDMYAKITGEDPYSTPMRIYPAVHYTMGGLWVDYE
                     LMTSVPGLYALGEANFSDHGANRLGASALMQGLADGYFVIPYTVGNYLAGEIRTAPVP
                     TSHAAFDAAEKEVADRLNKLKNINGTQSPESFHKRLGKIMWDKCGMARNEAGLKEAIQ
                     EIRALKAEFWKDLRVTGEIDGFNPELDKAGRVADFIELGELMCEDALQRRESCGGHFR
                     EEMQTEDGEARRDDANFSFVAAWEYKGDSSWEMHKELLQFEVAHPSQRSYK"
     gene            1180..1620
                     /locus_tag="VKQ52_15930"
     CDS             1180..1620
                     /locus_tag="VKQ52_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88746.1"
                     /translation="MIKMELVEWVAKVIAGVGAGLGITIAAFNYRHQTRIKQAEWLKS
                     LFEKFFEGSMYKEVRVWLDYGNLHQRLTVDDAGQRQQNEEKFTDFLNFFEFIGVLHAR
                     GHLPLEQVRDVFDYYLRKIGSDADCREWIDKYSFEKLKALLARI"
     gene            1617..2030
                     /locus_tag="VKQ52_15935"
     CDS             1617..2030
                     /locus_tag="VKQ52_15935"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HLZ88747.1"
                     /translation="MKNYLFVYGTLRKNYDLKLKEKVKGGLAYVGQAKIGAALYDIGR
                     YPGAVKDRTGKEVVGDVFLVSDPAAVFGVLDKYEGFEERRAADSEFVRRKGKVRLRGG
                     KELNAWIYWYNFDPAGKTMIRYKDYLNYLKNKSIH"
BASE COUNT          464 a          439 c          722 g          418 t
ORIGIN      
        1 cgggggatca ccagtcgaag ctgaccctga tgtcggagtc cctgcgtaac gatggccgta
       61 tctgggtgcc gaagaagaag gatgatccgc gcaagcccaa cgagatcccc gctgaggaga
      121 gagactatta tctggaacgc cgctatccgg cctttggcaa cctggttccc cgggacgtgg
      181 cgtcacgcgc cgcgaaggaa cgttgcgatg ccggttatgg ggtgggcacg tcgaagcagg
      241 cggtctacct ggattttgct tcggcgatcg aacgctatgg gaagaccgaa tgccgcaagc
      301 acggcatcga caatcccacg gcggaggata tccgtacgca tggggtcgac gtagtaaagg
      361 agaaatatgg taaccttttc gatatgtacg ccaagatcac cggagaggat ccctatagca
      421 cgccgatgcg gatctacccg gcggtgcact atacgatggg cggtttgtgg gtggattatg
      481 agctgatgac ctctgttccg gggctgtatg cgcttggcga ggccaatttc agcgatcatg
      541 gcgccaaccg gctgggggct tccgcgctga tgcaggggct tgccgacggc tattttgtga
      601 tcccgtatac ggtggggaat tacctggcgg gggagatccg gacggcgcct gttcccacgt
      661 ctcatgcggc tttcgatgcg gcggagaagg aagtggccga caggttgaac aaactgaaga
      721 atatcaatgg cacgcaatcg ccggaaagct ttcacaagcg cctggggaag atcatgtggg
      781 ataaatgcgg gatggcgcgt aatgaggcgg gcctgaagga ggcgatccag gagatccggg
      841 cgttgaaggc cgagttctgg aaggatctgc gggtgacggg ggagatcgac gggttcaatc
      901 ccgagctgga taaggccggc cgcgttgccg actttatcga actgggggaa ctgatgtgcg
      961 aggacgcctt gcagcgcagg gaaagctgtg gggggcattt ccgtgaggag atgcagaccg
     1021 aggacggcga ggcccggcgc gatgatgcca atttcagttt tgtggccgcc tgggaataca
     1081 agggggacag cagctgggaa atgcataagg agctgctgca gttcgaggta gcgcatccga
     1141 gccagcggag ctacaagtag ggaggaaaac gcttattcta tgataaaaat ggaattggtg
     1201 gaatgggtgg ccaaggtgat cgccggggtg ggggcggggt tagggataac catcgccgcg
     1261 ttcaactacc ggcatcagac gcggatcaag caggccgagt ggttgaagtc gttgttcgag
     1321 aaattctttg aagggtcgat gtataaggaa gtgcgggtct ggctggacta tgggaacctg
     1381 catcagcggc tgaccgtcga tgatgcgggc cagcggcagc agaatgagga gaagttcacg
     1441 gattttctca acttctttga atttatcggg gtcttgcatg cgagggggca tctgccgctg
     1501 gagcaggtgc gggatgtttt tgattactat ctccgcaaga tcggttcgga tgcggattgc
     1561 cgggaatgga tcgataaata cagttttgag aaattgaagg cgcttttggc ccggatatga
     1621 agaactattt atttgtatat ggaaccctgc ggaagaacta cgatctgaag ctgaaggaaa
     1681 aggtgaaggg cggcctggcg tatgtggggc aggcgaagat cggggcagcc ctgtatgata
     1741 tcggccggta cccgggggcg gtgaaggacc ggacgggaaa ggaggtggtc ggggatgttt
     1801 tcctggtgag tgacccggcg gccgtgttcg gggtgttgga caaatatgag ggattcgagg
     1861 agcggcgggc ggccgattcg gagtttgtcc gcaggaaggg gaaggtgcgg ctgcgcggcg
     1921 ggaaggagtt gaatgcctgg atctactggt acaacttcga tccggcggga aagacgatga
     1981 tccggtacaa agattatttg aactatttga aaaataaaag tatccactaa gcgaatttta
     2041 tgg
//
LOCUS       DATGFG010000846         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1131635, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000846 DATGFG010000000
VERSION     DATGFG010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131635"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..689)
                     /locus_tag="VKQ52_15940"
     CDS             complement(15..689)
                     /locus_tag="VKQ52_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLZ88748.1"
                     /translation="MEKEYIVKILETRQVTHDVREFRVEKPKGYTFIPGQATDVTINK
                     PGWKEKKNPFTFTGLNDDPYLQFTIKRYPDHHGVTDQLHQLQPGDTIILHDVWGAIEY
                     HGPGVFLAGGAGITPFLAIFRHLHRDHKADGNTLFFSNKTAEDIIEHKELLDILGVSA
                     NFILTNDPKAESYLHARIDKHFLQEQITDFRQPFYVCGPDQMVIDLSNTLKELGADPE
                     FLVFEK"
     gene            complement(708..1922)
                     /locus_tag="VKQ52_15945"
     CDS             complement(708..1922)
                     /locus_tag="VKQ52_15945"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLZ88749.1"
                     /translation="MSLLKSFGKAPAGPRLQRISRSSHYRDGSFQNLVPTTVTRKDAN
                     FLKMLKEYRNRPADTAPPKPLPSIRTDLRTLPSGQASIVWFGHSSYLLKLDTTHILVD
                     PVFSGNASPVSFFAKAFPGADVYKPGDMPDRLEAILLTHDHYDHLDYKTILQLKHRTK
                     HFYTSLGVGAHLEFWGIPTEKITELDWGETVAIDPAGNPLPATDANSTSIYLTATPAR
                     HFSGRGLKRGGAAWSSFVLNAPNPNSPNLNSPNPNIPNPNSSNPNSPNFNSPATNTSD
                     PTHRRLNLFLGGDSGYESHFRSIGETYGPFDLAILECGQYGVNWPYIHMQPEQTVQAA
                     LDLKAATLFPVHWAKFTLALHPWDEPIRRVKTAAGKAGLPITTPRIGEPVIINHDYPV
                     DPWYDLNRMESI"
     gene            complement(1979..>3119)
                     /locus_tag="VKQ52_15950"
     CDS             complement(1979..>3119)
                     /locus_tag="VKQ52_15950"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLZ88750.1"
                     /translation="FVNEYISKGWINGVVTLVAKNGHVVQYKGYGFADKEAGKPMRRD
                     DLFRIASQTKAIVSVGIMQLFEQGKFYLDEPIADFIPAFRDTKVLDKFNAADSTYTTV
                     PAKRLITFRDLLTHTSGLDYSLIGTKEGTAIYAKAGIPVGLGDFDYSLRERVDALAHL
                     PLTNQPGEKWMYSLSVDVLGCLIEVISGRNLEDYLVEHLFSPLGMNDTRFNIPAAKAG
                     RLTTAYTEDSLKHVIPWTKQRTGIDPSYPLAKKRYFSGGAGLTSTAWDYAIFLQMLLN
                     GGTYNGARVLAPRTVDMMTSGQLPFLYNGADNFGLGFDITSARTAAREVRSEGSISWG
                     GFFGTTYWADPKEHLVCLIMTQQNPNSHSDFEHRLEQIIYSSFTR"
BASE COUNT          532 a          640 c         1202 g          745 t
ORIGIN      
        1 gaaggcgcgg ggtattattt ttcgaagacg aggaattcgg gatcggcgcc gagttccttc
       61 agggtgttgg aaaggtcgat gaccatctgg tcgggtccgc agacgtaaaa gggttgccgg
      121 aagtcggtga tctgttcctg gaggaagtgt ttgtcgatac gggcgtggag gtagctttct
      181 gccttggggt cgtttgtgag gatgaagttg gcgctgaccc cgaggatatc gaggagttcc
      241 ttgtgttcga tgatgtcttc cgcggtcttg ttggagaaga agagggtgtt cccgtcggct
      301 ttgtggtccc ggtggaggtg ccggaaaatg gccaggaagg gggtgatgcc ggcgccgccg
      361 gcaaggaaga cgccgggtcc gtggtattcg atggcgcccc agacatcgtg gaggatgatg
      421 gtatcgccgg gttgcagttg gtgcaactgg tcggtgacgc cgtgatggtc ggggtagcgt
      481 ttgatggtga attggaggta ggggtcgtcg ttcagtccgg tgaaggtgaa gggatttttc
      541 ttttccttcc atccgggttt gttaatcgtc acgtcggtag cctggccggg gatgaaggtg
      601 tagccttttg gcttttccac gcggaattcc ctgacgtcgt gggtgacctg tcttgtttcg
      661 aggatcttta cgatgtattc cttttccatg tggtgtgttt gtgtacgtta tatggattcc
      721 atccggttta gatcgtacca ggggtcgacg gggtaatcgt ggttgatgat gacgggttcg
      781 ccaatccttg gagtggtgat ggggaggccg gcttttccgg ccgcggtctt tacccggcgg
      841 atgggttcgt cccaggggtg gagggccagg gtaaatttcg cccagtggac ggggaagagg
      901 gtggcggctt tgaggtcgag agcggcctgg acggtctgtt cgggttgcat gtggatatag
      961 ggccagttga cgccgtattg gccgcattcg aggatggcga ggtcgaaagg gccgtatgtt
     1021 tcgccgatgg accgaaagtg ggattcgtag ccggagtcgc cgccgaggaa gaggttgagg
     1081 cgccggtgcg ttgggtcgga ggtgtttgtt gcgggggagt tgaagttggg ggagtttggg
     1141 ttggatgagt ttggattcgg tatgttggga ttcggggagt ttaggttggg ggagtttgga
     1201 tttggggcgt ttaggacgaa ggaggaccag gcggcgccgc cgcgtttgag gccgcggccg
     1261 gagaagtggc gggcgggggt ggcggtgaga tagatggacg tggagttggc gtcggtggcg
     1321 ggcaggggat taccggcggg gtcgatggcg acggtttctc cccagtcgag ttcggtgatc
     1381 ttttcggtgg ggatgcccca gaattcgagg tgggcgccga caccgagtga ggtgtagaaa
     1441 tgtttggtcc ggtgctttaa ctggaggatc gttttataat cgaggtgatc gtagtggtca
     1501 tgggtgagga ggatagcctc gagcctgtcg ggcatgtccc cgggtttgta gacatcggct
     1561 cccgggaagg cttttgcgaa gaaggagacg ggggaggcgt tgccgctgaa taccgggtcg
     1621 accaggatat gggtggtatc gagttttaaa agataggagg agtgaccgaa ccagacgatg
     1681 gaggcctgcc cggatggaag ggtccggagg tcggtacgga tcgacggaag gggctttggc
     1741 ggggcggtgt cggccgggcg attgcgatat tccttcaaca ttttcaggaa gttcgcgtcc
     1801 tttcgcgtga ccgtcgtggg cacgaggttc tggaagctgc cgtcgcgata atgcgaggaa
     1861 cggctgatgc gttgcagccg gggcccggcg ggggccttgc cgaaggattt caatagtgac
     1921 atggatgttg taacaatgaa atggggattt ggttgagccg cggagcgctt ccaggaagtt
     1981 aacgggtgaa gctcgaatag atgatctgtt cgaggcggtg ttcgaaatcg ctgtgggagt
     2041 tggggttttg ctgggtcatg atgaggcaga cgaggtgttc tttggggtcg gcccagtagg
     2101 tggtgccgaa gaagccgccc caggagatgg agccttcgct gcggacctcg cgggcggcgg
     2161 tgcgggcgct ggtgatgtcg aagccgaggc cgaagttgtc ggcgccgtta tagagaaagg
     2221 gcagttggcc gctggtcatc atgtcgacgg tgcggggagc gaggacgcgg gctccgttgt
     2281 atgtgccgcc gttgaggagc atctggagga agatcgcata gtcccaggcc gtggaggtga
     2341 ggccggcgcc gcccgagaag tagcgttttt ttgcgagggg atagctgggg tcgatgccgg
     2401 tgcgttgttt tgtccagggg atcacgtgtt tgagggagtc ttccgtataa gccgtcgtga
     2461 gtcggccggc cttggccgcc gggatattga accgggtgtc gttcatgccg agggggctga
     2521 acaggtgttc gacgagatag tcttcgaggt tcctgccgct gatgacctcg atgaggcagc
     2581 cgagcacatc cacgctgagg ctgtacatcc atttttcacc gggttggttg gtgaggggca
     2641 gatgcgcgag ggcatcaacg cgttcgcgga gactgtagtc gaagtcgccg agaccgacgg
     2701 ggatcccggc ctttgcatag atggccgtgc cttcctttgt gccgatgagc gaatagtcga
     2761 gaccggaggt gtgggtgagg agatcgcgga aggtgatgag ccgttttgcg ggaacggtcg
     2821 tgtacgtcga gtcggcggcg ttgaatttat cgagaacctt tgtgtcgcgg aaggccggga
     2881 tgaagtcggc gatgggttcg tcgagataga atttcccctg ttcgaagagt tgcatgatgc
     2941 cgacgctgac gatggccttt gtctggctgg cgatgcggaa gaggtcgtcg cggcgcatgg
     3001 gtttgcccgc ttccttgtcg gcgaagccgt agcccttgta ttgcacgaca tggccatttt
     3061 ttgcgacgag ggtgacgacg ccgttgatcc agcccttgct gatatattcg tttacgaag
//
LOCUS       DATGFG010000847         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1426367, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000847 DATGFG010000000
VERSION     DATGFG010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426367"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VKQ52_15955"
     CDS             <1..1009
                     /locus_tag="VKQ52_15955"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HLZ88751.1"
                     /translation="LLRQVIFAVMVLFCLTPLVTPPLALVLGLFIALFVGHPFLHLNH
                     KATHILLQVSVVGLGFGMNAAAALKAGREGFLFTVVSILGTLGFGLVLGRMMKIDRKT
                     SLLISGGTAICGGSAIAALAPVIRAEERQVSVALGAVFILNSVALFLFPVVGHLVGLS
                     QAQFGLWSAIAIHDTSSVVGAAGRYGPEALQIATTVKLARALWIIPVSLVAAFVFRGP
                     KRGAVQDGAGEAAGRVGAGGRGKRVGTARRGKVRVKIPYFIGLFVVAILVNSWLPMRA
                     VSRVIVGASHAGLTLTLFLIGSGLSRQVLKNTGVKPLVQGVVVWVLIAGATLWAVMGL
                     A"
     gene            1082..1642
                     /locus_tag="VKQ52_15960"
     CDS             1082..1642
                     /locus_tag="VKQ52_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88752.1"
                     /translation="MSQSTVVTLPISEEDEALLRKLQEKAWFTFLRLYLPLFLFLLYI
                     YFRMEPGGVFRGRYLSYGKMTHAGYALVYMIFAVVFGVIFLVFAIRDYKRLILPFQQE
                     LRMRSKYRFAFAARKYHDPIYDTCLLFYPGKENLYIKVDKKDFESIDNGEELRLEIAA
                     VTGEVLSLRSGNRDFYRPEEFSFSDR"
     gene            complement(1802..>2794)
                     /locus_tag="VKQ52_15965"
     CDS             complement(1802..>2794)
                     /locus_tag="VKQ52_15965"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HLZ88753.1"
                     /translation="QTLEEADWCIEQTLFFGGTDRPLNMILDDGGDLTNIVFDKYPEL
                     VQHIKGLSEETTTGVHRLYERMEKGTLPIPAFNVNDSVTKSKFDNKYGCKESLVDAIR
                     RATDVMMAGKVAVVGSYGDVGKGSAASLKGAGCRVIVTEIDPICALQAAMDGFEVKKM
                     VDAVKEADIVVTASGCRDLITEKHFRSMKDKAIVCNIGHFDIEIDMAWLNKNYGHTKD
                     TIKPQVDVYNIDGKDVIVLAEGRLVNLGCATGHPSFVMSNSFSNQTLAQLELWTNHKK
                     YENKVYVLPKLLDEKVARLHLAKIGVELDELTDAQATYLGIPKAGPFKPEHYRY"
BASE COUNT          485 a          613 c          974 g          722 t
ORIGIN      
        1 tcttctccgg caggtgatct ttgcagtgat ggtgttgttt tgtttgacgc cgctggtgac
       61 gccgccgctg gcactggtgc tggggttgtt tatcgccctt tttgtcgggc atcctttttt
      121 acatctgaat cataaggcta ctcatattct cctgcaggtg tcggtggtgg ggcttggttt
      181 tgggatgaat gccgctgccg ccctgaaggc cgggcgggaa ggttttcttt ttacggtggt
      241 ctcgatcctc gggacccttg gttttgggct tgtgctgggc aggatgatga agatcgaccg
      301 gaagacctcg ttgttgatct cgggagggac ggccatttgc ggcggtagtg ccatcgcggc
      361 gctggccccg gtgatcaggg cggaggagcg ccaggtttcg gtggcgctgg gggcggtgtt
      421 tatcctgaat tcggtggcgt tgttcctgtt ccccgtggtg ggtcacctgg ttgggttgag
      481 ccaggcgcag tttgggttgt ggagtgcgat cgcgatccat gatacgagtt ctgtggtggg
      541 ggcggcgggc aggtatgggc cggaggcgtt gcagatcgcg acgacggtga agctggcgcg
      601 ggcgctctgg atcatcccgg tgtcgctggt ggcggcgttt gtcttccgcg ggccgaagag
      661 gggagcggtg caggatggag ccggggaagc agccgggcgt gttggcgcag gagggcgagg
      721 gaagcgtgtt ggcacagcca ggcgagggaa ggtgagggtg aagatccctt attttatcgg
      781 gttgtttgtg gtggcgatcc ttgtgaacag ctggttgccg atgcgggcgg tgagccgggt
      841 gatcgtcggg gcttcacatg cggggttgac gctgacgttg tttttgatcg ggagtgggtt
      901 gtcgaggcag gtgttgaaaa atacgggggt gaagccgctg gtgcagggag tggtggtatg
      961 ggtgttgatc gcgggggcga cgttgtgggc ggtgatgggg ttagcgtgat agagggtgcc
     1021 ggtggggttg ttgcatttgt taggggcaat gagtatattg gaagaataaa ctccaaatcc
     1081 gatgagccaa agtacggttg ttacgttgcc tatttccgaa gaggatgaag ccctcttgcg
     1141 gaaattacag gagaaagcgt ggttcacctt tctgcgcttg tatctgccgc ttttcctttt
     1201 cctgctttat atctatttcc ggatggagcc gggcggggta ttccgcgggc gttatctgtc
     1261 ctatggaaag atgacccatg cggggtatgc cctggtgtat atgatctttg cggtggtgtt
     1321 tggcgtgatc ttcctggtct ttgcgataag agactataaa cggctgatcc tgccttttca
     1381 acaggagttg cggatgcgca gtaaataccg ttttgcgttc gcggcgcgga agtatcacga
     1441 ccctatctat gacacatgcc tgctcttcta cccgggtaag gaaaacctgt atatcaaagt
     1501 agataaaaag gatttcgagt ctatcgacaa tggggaagag ctacgattgg agattgccgc
     1561 agtgacgggg gaggtgcttt cgctcagaag cgggaaccgg gatttctaca ggccggagga
     1621 gtttagtttt tcagacagat aagcagtccg aaaggatgtc aaataaaaaa gcccgcttcg
     1681 gtttgaagcg ggctgtatgt tttgtcgcgg tgctgccggg atgtcgccgg atgttgcccg
     1741 tgacgttgtc gggatgttgc cggatgccgc cggatgttgc ccggctggcg taccgaaacg
     1801 actagtaccg atagtgctcg ggtttgaacg gaccggcctt ggggataccg agataggtag
     1861 cctgggcgtc ggtgagttcg tcgagttcaa cgccgatctt ggcgaggtgg aggcgggcta
     1921 ccttctcgtc gaggagtttg ggcagcacat acaccttgtt ctcgtatttc ttatggttgg
     1981 tccacagttc gagctgcgcg agggtctggt tggagaagga gttgctcatc acgaaggagg
     2041 ggtggccggt ggcgcagccc aggttgacga ggcggccttc agccagtacg atcacgtcct
     2101 tgccgtcgat gttgtagacg tcgacctggg gcttgatggt atcctttgtg tggccatagt
     2161 tcttgttgag ccatgccatg tcgatctcga tgtcgaagtg gccgatgtta caaacgatgg
     2221 ccttatcctt catcgagcgg aagtgttttt ccgtaatgag gtcccggcag ccggaagcgg
     2281 tgacgacgat gtcggcttct tttacggcgt ccaccatttt ctttacttcg aaaccgtcca
     2341 ttgcggcctg gagggcgcag atggggtcga tctcggttac tataacgcgg cagcccgcgc
     2401 ctttgaggga agcggcggag cctttaccaa cgtcgccata gctacctacg acggcgacct
     2461 tgccggccat cattacatcg gtagcgcgac ggatggcgtc gacgagggat tctttacaac
     2521 cgtatttgtt gtcaaatttg gacttggtga cggagtcgtt gacgttgaac gcggggatgg
     2581 ggagggtacc cttttccatg cgctcataca ggcggtgaac accggtggtg gtctcttcgc
     2641 tgaggccctt gatatgctgt accagttcgg ggtatttgtc gaaaacgatg ttggtgagat
     2701 cgccaccgtc gtcgaggatc atattcaggg ggcggtcggt gccgccgaag aagagggttt
     2761 gctcgatgca ccagtcggct tcttcgaggg tctg
//
LOCUS       DATGFG010000848         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_236649, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000848 DATGFG010000000
VERSION     DATGFG010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236649"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VKQ52_15970"
     CDS             <1..180
                     /locus_tag="VKQ52_15970"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HLZ88754.1"
                     /translation="SYIRASKPDIAIPLYEQTIKTLTTHLGKPIGTGEFGADMKVELL
                     NDGPVTILIDTKNKE"
     gene            complement(230..907)
                     /locus_tag="VKQ52_15975"
     CDS             complement(230..907)
                     /locus_tag="VKQ52_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88755.1"
                     /translation="MKILIVEDEIDLAQSMATYLKGENYLCELAPDIRTALEKIELFE
                     YDCILLDISLSDGNGLKVLKELKANNKTDGVIILSARNSIDDKIDGLNLGADDYLSKP
                     FHLPELNARIAAVIRRRRFEGNKTLVLNELTIDLIGKTVVVGNEPVDLTRKEYDLLLY
                     LASNKNRVISKNAIAEHLSGEQAMSYDNLDFIYSHMKNLKRKLHQAGCGDYIRSLYGM
                     GYKFEVR"
     gene            976..2124
                     /locus_tag="VKQ52_15980"
     CDS             976..2124
                     /locus_tag="VKQ52_15980"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HLZ88756.1"
                     /translation="MQIYQVDAFANQIFQGNPAAVIPLTEWPPDTLLRQIAAENNLSE
                     TAFFVPEKEGFAIRWFTPAVEVDLCGHATLAAGHVLFEHLGYPGDHILFHTRNAGDLE
                     VLRGDAPGRLTLNFPDNLPEPVDPAPLTVIFESLHLPPGPIAKGPYDYFIVLHSQKAV
                     EALRPDFKRLAAAGGRGIVVTAPGEDTDFVSRCFYPQSGIDEDPVTGSAHTMSVPYWA
                     SRLGKNKLSAAQLSARRGYLDCELRQGRVYMTGNAHTFLQGTITLPGIKPATPTLTIH
                     ETQQRIDRWIKTTGVRYFSELTNMAILTEEVGEVARLMSRLYGDQSFKESDKDKQLAD
                     ELADVLWVLCCIANQTGIDLTDALQKNFTKKNIRDGQRHIANDKLKPG"
     gene            2175..>3484
                     /locus_tag="VKQ52_15985"
     CDS             2175..>3484
                     /locus_tag="VKQ52_15985"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HLZ88757.1"
                     /translation="MANDTNRFQAIISHCKEYGFIFPSSEIYDGLSAVYDYGQWGSEL
                     KKNIKDHWWKSMTQLNENVVGIDAAIFMHPTTWKASGHVDSFSDPMIDNKDSKKRYRV
                     DHLIEARIEELEKEGKNTEAAALQADMDRLLAASDLAGLKALIESRKIKCSVSGTCNW
                     TDVRDFNLMFSTQLGSVSDEASEIYLRPETAQGIFVNFLNVQKTGRMKIPFGIAQIGK
                     AFRNEIVARQFIFRMREFEQMEMQFFIRPGTQNEWYEYWKEARMKWHLGLGIPAEKYR
                     FHDHVKLAHYAKAACDIEYEFPIGFKEVEGIHSRGDYDLSRHQEFSRKKLQYFDPEIN
                     QNYVPYVIETSIGLDRTVLMVLSEAYTEEDLSTPERSDSRVVLRFPPRLAPIKLAILP
                     LTKKDGLPELAREIMDICKPQFRCFYEEKDTIGKRYRRMDAIGTP"
BASE COUNT          889 a         1133 c          809 g          653 t
ORIGIN      
        1 tcctatatcc gggccagcaa accggacatc gccatccccc tctacgaaca gacgataaaa
       61 accctgacca cccacctggg aaaacccatc gggacaggcg aattcggcgc agatatgaaa
      121 gtggaattgt tgaacgacgg accggtaacg atcctcatcg acacgaagaa caaagagtaa
      181 gggaagaaca aagagtaact cccgaggggc gggaccccga acagacggcc tatcttactt
      241 caaacttata ccccatccca taaagactgc ggatataatc cccgcaaccc gcctggtgca
      301 gcttccgctt caggttcttc atatgggaat agataaaatc caggttgtca tagctcatcg
      361 cctgctcacc ggaaagatgc tcggcaatcg cattcttgga aatgaccctg ttcttattcg
      421 aagccagata cagcaacaga tcgtattcct tacgcgtcag atccaccggc tcattcccta
      481 caaccacggt cttgccgatc agatcgattg tcaactcatt taaaaccagt gttttatttc
      541 cttcgaaccg ccgccgccgg atcaccgccg cgatccgcgc attcaactcc ggcagatgaa
      601 agggcttgct caggtaatca tcggccccca ggttcaaccc gtcgatcttg tcgtcgatgg
      661 agttccgggc cgaaaggatg atcaccccat ccgtcttgtt attggccttc aattccttca
      721 acaccttcaa cccattccca tccgacagcg aaatatccag caaaatgcag tcatattcaa
      781 ataactctat cttttccaat gcagtacgga tgtcgggagc cagctcgcaa agatagttct
      841 cccccttcag gtaggtagcc atactctggg cgagatcgat ctcgtcttcg acgatcagta
      901 ttttcatctg caaaaataac gacaacattc tgtacggata ctggaatcgt atatttgtcc
      961 aaaagcggac tatccatgca gatctatcag gtcgacgcct ttgcaaatca aatcttccag
     1021 ggtaacccgg ccgccgtcat cccccttacg gaatggccgc ccgacaccct cctccggcag
     1081 atcgccgcgg agaacaacct ttccgagacc gcctttttcg tcccggaaaa agagggcttc
     1141 gccatccgct ggttcacccc cgccgtggaa gtagacctct gcggccacgc caccctggcc
     1201 gccggccacg tcctgttcga acacctgggc taccccggcg accacatcct cttccatacc
     1261 cgcaacgcag gcgatctcga agtactgcgg ggcgatgctc ccggccgcct gaccctgaat
     1321 ttcccggata acctccccga gcccgtcgac ccggcccccc tgacggtcat cttcgaaagc
     1381 ctccatctgc cacccggccc catcgcaaaa ggcccctatg attatttcat cgtcctccat
     1441 agccaaaaag ccgtcgaagc cctccggccg gatttcaaac ggctcgccgc cgccggcggc
     1501 cggggtatcg tcgtcacggc tccgggagaa gacaccgact tcgtatcccg ctgcttctat
     1561 cctcaatcgg gcatcgacga agaccccgtc accggctcgg cccataccat gtcggtaccc
     1621 tactgggcaa gccgcctggg aaagaataaa ctctccgccg cccagctctc cgcccgccgc
     1681 ggctacctcg actgcgaact gcgccagggg cgggtataca tgactggtaa tgcccacacc
     1741 ttcctccagg gcaccatcac gctgccgggc ataaaaccgg ccacacccac cctcaccatc
     1801 cacgaaaccc agcaacgcat cgatcgctgg ataaaaacca ccggcgtccg ctatttcagc
     1861 gaactgacaa acatggccat cctcaccgaa gaggtcggcg aggtcgcccg cctgatgagc
     1921 cgcctctacg gggaccagtc cttcaaagaa tccgataagg ataaacaatt agccgacgaa
     1981 ctggccgacg tcctctgggt actctgctgc atcgccaatc aaaccgggat cgatctcacc
     2041 gatgccctgc aaaagaactt caccaagaag aacatccggg acggccaacg acacatcgcc
     2101 aatgacaagc tcaaaccggg ctaaatgatt tatatttgcc aactatggcc gccgcaaacg
     2161 gcccggtata tattatggct aacgatacga acaggttcca ggcgatcatt tcgcattgta
     2221 aagagtatgg tttcattttc ccttcaagcg agatctatga cggcctcagc gccgtatacg
     2281 actacggcca gtggggcagc gaactaaaaa agaatatcaa ggaccactgg tggaaaagca
     2341 tgacccagct caacgaaaat gtcgtcggta tcgacgccgc catcttcatg caccccacca
     2401 cctggaaggc cagcggtcac gtcgatagct tcagcgatcc gatgatcgac aataaagaca
     2461 gcaaaaaacg ctaccgcgtc gatcacctca tcgaagcccg tatcgaagaa ctggaaaaag
     2521 aaggaaagaa caccgaagcc gccgccctcc aggcggacat ggacaggctc ctcgccgcaa
     2581 gcgacctggc aggcctcaaa gcactcatcg agtcccggaa gataaaatgc agcgtcagcg
     2641 gaacctgcaa ctggaccgat gtccgggatt tcaatctcat gttctccacc caactgggca
     2701 gcgtatccga cgaggccagc gagatctacc tccgccccga gaccgcccag ggtatcttcg
     2761 ttaacttcct caacgtccag aaaacaggcc gcatgaagat ccccttcggc atcgcccaga
     2821 tcggcaaggc cttccgcaac gagatcgtcg cccggcaatt catcttccgc atgcgggagt
     2881 tcgaacagat ggaaatgcag ttcttcatcc gtcccggcac acagaacgaa tggtacgagt
     2941 actggaaaga agcgcgcatg aaatggcatc tcggcctggg catccccgcg gaaaaatatc
     3001 gcttccacga tcacgtcaaa ctcgcgcact acgcaaaagc cgcctgtgac atcgaatacg
     3061 aattccccat cggcttcaag gaggtggagg gcatccactc ccgcggtgac tatgacctga
     3121 gccgccacca ggaattcagc aggaagaaac tccagtactt cgaccccgag atcaaccaga
     3181 actatgtccc ctacgtcatc gaaacctcca tcggtctcga ccgcaccgtc ctcatggtgc
     3241 tgagcgaagc ctatacggaa gaagacctga gcaccccgga aagatccgat agccgcgtgg
     3301 tcctgcgatt ccctcccagg ctggcgccaa taaaactggc catcctgccg ctgaccaaaa
     3361 aggacggcct gcccgaattg gcccgggaga tcatggacat ctgtaaaccc cagtttcgct
     3421 gcttttacga ggaaaaagat accatcggta aacgctaccg ccggatggac gccatcggca
     3481 cacc
//
LOCUS       DATGFG010000849         4454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_516031, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000849 DATGFG010000000
VERSION     DATGFG010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516031"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="VKQ52_15990"
     CDS             complement(<1..1653)
                     /locus_tag="VKQ52_15990"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HLZ88758.1"
                     /translation="MRQPAKSIARKVIYLFLYISLQSTHPAHGQTTAHSQTPAIINLK
                     CEYLTNPLGIDNPNPRITWQLRDAEQDIHQTVYQILVGTDSAAVANGHGNTWASGTIN
                     NANNLITYNGIALQPFTKYYWTVVAETNKDHHLQSSVASFETGMMSQYNWKGAWISDG
                     ESIHVKPAPYFRKVFPVTKKILSARAYISAAGLYELYLNGKRIGDHQLDPMFTRFDRR
                     NLYVTYDVTQILQEGRNAVGVLLGNGWYNFQTMAVWDYERAPWRSRPAFCLDLRITYD
                     DGSVETVTSDQSWKTALSPLVYNSIYTGEQYDARKELPGWNTVNFDDGKWHRAKYRES
                     PSNNIVAQTLWPIRHVEKLQPVSVTRTSDTSWLYDFGRNIAGISELTIAGDSNTRVRV
                     SHAERLDKKGKIDQSNVDYFLSAETRSDDPFATDSYRLSGKGRQTFTPRFSYKGFRYA
                     EVTSSRPLSLGREDLVAWSMHSDVPAVGKITTSSDLINKIYAATNASYLANLFGYPTD
                     CPQREKNGWTGDAHIASETGLYNFDAITIYEKWLADLRDEQQP"
     gene            1688..2704
                     /locus_tag="VKQ52_15995"
     CDS             1688..2704
                     /locus_tag="VKQ52_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88759.1"
                     /translation="MRKIVVMSLPALLVFLFTVASFSLFGQDGRRAAGGNLVLFGRGL
                     YPGDDSSFDLIRHSGFTTLMLSSFYIRSDGDVYSGDDSHHPIIHQGVFTGSESWAKRV
                     RALRKHGSPIRRIEILLEGRWYHQPPNTYDFIRDWIDGGATEPGVIPGTKTGSTLYTI
                     ARILKEEIGADAICIDDESVYDSTSIIQLGKMLQRIGLRMTICPYTNIPYWKKIVDSS
                     SRAVVDAAYLQCYDGGAHATPGPWAEGLQHTIPVYPIFLCRGAFSTCGSSHNSKTPEE
                     ISAMMRAFRKEYPGMSGAGIWQMQDVKSYVHMDCAVQDPASGNAKSVEEYLEQLRRSL
                     GEGL"
     gene            2822..3370
                     /locus_tag="VKQ52_16000"
     CDS             2822..3370
                     /locus_tag="VKQ52_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ88760.1"
                     /translation="MHTERKNAIRRTVIDTASRLFYKQGYANTGINQIIEESGVVKSS
                     VYTAFRTKEDILMAYLETTGAATDKALQEAADRFPTPREKVLAVFDYLIALVQQEEYY
                     GCQFLNIISEIPLETERVIKRIQKQKNNVRSLFSRLLEPIGKQGLADEIYLLFEGALI
                     ANKVHHAVWPVEAARKIASRLV"
     gene            3713..>4454
                     /locus_tag="VKQ52_16005"
     CDS             3713..>4454
                     /locus_tag="VKQ52_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ88761.1"
                     /translation="MKKNTIRYREIRVDGLRLFYREAGDRAKKAIVLLNGVPNASSAF
                     QELMNELKEDFYLVAPDFPGFGNSEIPDKDKYEYSFHQLSVTIEHFIDALGLQHPHVY
                     ALGYGGPITFRIALRRPGLFAGYILQNSNAYEEGLGPAMQDAAPYLANRNEETEKLVK
                     PLLTLDGMKLFFLNGAKEPQAINPDGYTSALYYLSRPGQQEIQLDLLYDYRNNLAEYP
                     KWQQWLKTTQPRMLLVWGKNDVFFPVSAA"
BASE COUNT          929 a         1048 c         1378 g         1099 t
ORIGIN      
        1 cggctgttgt tcatcccgca gatcggccag ccacttttcg tagatggtta tcgcatcgaa
       61 attatacagg ccggtctcac tggcgatatg cgcatcgccc gtccaaccgt tcttctcgcg
      121 ctgcgggcaa tcggtggggt agccgaacag gttggcgaga taggaggcgt tggtggccgc
      181 gtatatttta ttgatgaggt cactggaggt ggtgatcttc cctaccgccg gcacatcgct
      241 gtgcatggac caggcgacca ggtcttcccg gccgagcgac aaaggccgac tgctggttac
      301 ttccgcgtag cggaaccctt tatagctgaa gcggggggta aaggtttgcc ggcctttacc
      361 ggaaagccga tagctatccg tggcgaatgg atcatcggac cgggtctccg cggaaagaaa
      421 atagtcgacg ttcgactgat cgatcttccc ttttttatcc aggcgttcgg catgactgac
      481 ccggacccgg gtgttggaat cgccggctat ggtcaattcg ctgatcccgg cgatgttgcg
      541 gccaaaatca tagagccagg aggtatcgct tgtccgggtg acgcttaccg gctgcaattt
      601 ttccacgtgg cggatgggcc atagtgtctg cgccacgata ttgtttgacg gggactcgcg
      661 gtattttgcc cgatgccatt tgccgtcatc gaaatttacg gtattccatc ccgggagttc
      721 cttccgggca tcatattgtt cgcctgtata gatactattg taaacaaggg ggcttagcgc
      781 cgtcttccag ctttgatccg atgttacggt ttcgacggag ccgtcgtcat atgtgatccg
      841 cagatcgagg caaaatgcgg ggcggctgcg ccagggcgcc cgttcataat cccatacggc
      901 cattgtctgg aaattatacc agccgtttcc gaggaggacg ccgaccgcgt tgcggccctc
      961 ttgcaggatt tgtgttacat cgtaggtgac atacagattg cggcgatcga agcgggtgaa
     1021 catagggtcc agttggtggt cgccgatgcg cttaccgttg agatagagtt catataaccc
     1081 cgcggcggaa atgtaggccc tggcggacag gatctttttt gtgacaggga agacctttcg
     1141 gaaataaggc gccggcttta cgtggatact ttccccgtca cttatccatg cccccttcca
     1201 gttgtattga gacatcattc ctgtttcgaa gctggcgacg ctgctttgga ggtggtggtc
     1261 cttatttgtt tcggcgacaa ccgtccagta atattttgtg aagggctgca gggcgatgcc
     1321 gttgtaggtg atcagattgt ttgcgttgtt tatagtgcct gacgcccagg tattgccgtg
     1381 gccgttggcg acagccgccg aatcggtgcc taccaggatc tggtagaccg tttggtggat
     1441 gtcctgttcc gcgtcccgga gttgccaggt gatccgggga tttgggttat cgatgcccag
     1501 ggggttggtg aggtattcgc attttaggtt tattattgca ggagtttgcg agtgggcagt
     1561 tgtttgcccg tgggccggat gtgtggattg aagagagatg tacaggaaga gatatatgac
     1621 ttttcttgca attgatttgg cgggttgacg catatgccga aattatggaa ctttttgaat
     1681 tagcgatatg agaaagatag ttgttatgtc tctgccggcc ttgctggtat tcttgtttac
     1741 tgtagcttct ttttcccttt ttggccagga cgggcggcgt gctgccggcg gcaacctggt
     1801 cctgtttggg cggggcttgt atcccggcga tgattccagt tttgacctga tccgccattc
     1861 ggggtttacg acgctgatgc tgtccagctt ttatattcgt tcggacgggg acgtttattc
     1921 gggtgatgat tcgcaccatc ccattatcca ccagggggta ttcaccggca gcgaaagctg
     1981 ggcaaaaagg gtcagggcgc tccggaagca cggcagtccg atcaggcgta tcgagatcct
     2041 gctggaagga aggtggtacc accagcctcc caatacctat gacttcatcc gcgattggat
     2101 cgatggcggt gcgacggagc cgggggttat accgggaaca aagacaggga gcacgctgta
     2161 tacgatcgcc cggatcctga aggaagagat cggcgccgat gccatttgta tcgatgacga
     2221 atcggtctac gatagtacgt ccattatcca attgggaaaa atgctccagc gtatcgggct
     2281 tcggatgacc atctgtcctt ataccaatat cccctattgg aaaaagatcg tcgacagcag
     2341 cagccgggct gtggtggatg ccgcttacct gcaatgctat gatggagggg cgcatgctac
     2401 acccggtcca tgggcggagg gattgcagca tacgatccct gtgtacccca tctttttatg
     2461 ccggggcgcg ttcagtacct gcggcagttc gcataatagc aagaccccgg aggagataag
     2521 cgccatgatg cgggcgttca ggaaggaata tcccgggatg agtggggcgg ggatctggca
     2581 gatgcaggat gtcaagagct atgtccatat ggattgtgcg gtgcaggacc cggcttcggg
     2641 gaatgcgaag agtgtggagg aatatttgga gcagttgagg aggagtttgg gggaggggtt
     2701 gtaggttttg gggcggggag ttttggaggg gcggggagtt tagggggctg cgagtttgtg
     2761 aggctgtgag tccgtggcca aaacagactt gtctgtcgtt actattcagt agctttgttg
     2821 tatgcatacc gagaggaaga atgcgatcag gcggactgtt atcgatacgg cttcccggct
     2881 tttttataag caggggtatg ccaataccgg tattaaccag atcatcgagg agtccggggt
     2941 ggtcaagtct tctgtataca cggcctttcg taccaaggag gatatcctga tggcctacct
     3001 ggaaacgacg ggcgctgcaa ccgataaggc gttacaggag gctgctgatc gattccctac
     3061 accaagggaa aaagtgcttg ccgtatttga ttacctgatc gccctggtgc agcaggaaga
     3121 atattatggc tgccagttcc tgaatatcat ctccgaaata ccgctcgaga ccgagcgggt
     3181 tatcaaacgg atccagaaac aaaagaacaa tgtccgcagt ttgttttcga ggctcctgga
     3241 acccattgga aagcagggcc ttgcggatga aatttacctc ctttttgaag gcgcgctcat
     3301 cgccaataag gtacatcatg cggtttggcc ggttgaggcg gccaggaaga tcgccagccg
     3361 gttagtttag ataaaaatgg agcggagcct tgcgggggcg gggacttaag tgggtccctc
     3421 ggttggggcc ttggtgcgag gggccggtgc gggggcttta cgtgggggcc ttggtgtggg
     3481 ggctttggag gctttggggc ggttttgggg cgggcagaaa acagacttgt ttgttttgtc
     3541 tgtttgactt agttttgggg cgtgaatggg ttatagtggg gttggggcat tggcagtggg
     3601 gggtggagtt ggggggcgtg gccgcggaaa tgggcggcca gcaggcagag gcgggagatc
     3661 gcgggtcatg gcaaaaacaa gtcggtctgt ttttaatttg aaacaattca atatgaaaaa
     3721 gaatactatt cgctacaggg agatcagggt cgatgggctc aggctttttt accgggaggc
     3781 tggtgacagg gccaaaaaag cgatcgtgct gcttaacggc gtacccaatg cgtccagcgc
     3841 cttccaggaa ttgatgaacg agctgaagga agacttttac ctggtggctc ctgacttccc
     3901 gggatttggc aacagcgaga tcccggacaa ggacaaatac gagtacagct ttcaccagct
     3961 atcggtgacc atcgagcatt ttatcgatgc gctgggctta caacatcctc atgtgtatgc
     4021 actcggctat ggcggtccca ttacctttcg gatcgcctta cgccggcccg ggcttttcgc
     4081 cggttacatt ctgcagaatt ccaacgccta tgaagaaggg cttggcccgg ccatgcagga
     4141 tgccgccccc tatcttgcca accggaatga agaaaccgaa aaactagtaa agccgctgct
     4201 gaccctcgac ggaatgaagc tgttctttct gaacggtgca aaggaacccc aggccatcaa
     4261 tcccgatggt tacaccagcg ccctttacta tctcagtcgg cccggtcagc aggagatcca
     4321 gctggacctg ctctacgact atcgtaataa ccttgccgaa tacccgaaat ggcagcagtg
     4381 gttgaaaacc acccagcccc ggatgctcct tgtctggggc aagaacgatg tgttcttccc
     4441 cgtcagcgcc gccg
//
LOCUS       DATGFG010000850         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1152895, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000850 DATGFG010000000
VERSION     DATGFG010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152895"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2228)
                     /locus_tag="VKQ52_16010"
     CDS             complement(<1..2228)
                     /locus_tag="VKQ52_16010"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HLZ88762.1"
                     /translation="MDEKLQALGRSLEGELYYDKTMRTLYATDASAYREMPLAVAVPR
                     SVEDIRKLIAFARVERTSLIPRTAGTSLAGQVVGKGIVVDVSKYFTGILELNVEERWV
                     RVQPGVIRDDLNLFLKPHGLLFGPETSTANRAMIGGMVGNNSCGSNSVVYRSTREHLV
                     EVKALLSDGTDVVFGRIGLEEFHAKCEGSGLENSLYRETRRMLGNYDNQVGIREQFPK
                     KSIERRNTGYALDLLLDTAPFTAGCPDFNFCTLLAGSEGTLAFLTELKLDLSPLPPKE
                     TGLLCVHFNTIDESLRANLIALKYGPTASELIDHYILECTKDNIEQRKNRFFVQGDPG
                     AILVIEFKKDDREEIRKIAAAVEAEMRAAGLGYHFPLLFGADTGRIWSLRKAGLGLLS
                     NLPGDEKAVPVIEDTAVDVHDLPAYIRDFNEILKKYGLYSVHYAHAGSGEIHLRPIIN
                     LKTEEGNRLFRAIAEEIATLVKKYQGSLSGEHGDGRLRGEFIPQMVGEKNYQLFKELK
                     RSWDPVNIFNPGKIVDTPPMNTQLRYEPGQQTPPFPTLFRFHGQDILQHAEQCNGSGD
                     CRKTHLSGGTMCPSFMATRNEKDTTRARANILREFLTRSDKPNRYDNKEIYDVMDLCL
                     SCKGCKSECPSNVDVAKLKAEFLQHYYDANGVPMRSRMIASFSKSAQLGMLVPTVYNL
                     FVRQNWVKKLVGFAPERSMPLLYKTTLKRWWKKRSKAGSGKKLYLFCDEFTNYNDVEI
                     GM"
     gene            2227..3531
                     /locus_tag="VKQ52_16015"
     CDS             2227..3531
                     /locus_tag="VKQ52_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88763.1"
                     /translation="MIAKIHPFTYFCPMYRIFIVAACLGLILPEAPANAQKIQQLQPK
                     PALLRLIDGNLRQAVNQYKVLMGRVPADRLPKTWYANSDKLETSDADWWTSGFYPGTL
                     FYLYEFSHDTTLLAEAQRRLTLLEKEQYNKGTHDLGFMMYCSFGNALRLIQQELLHNP
                     AAPYDSILLTSARSLSTRFHPAVGCIKSWDSKPWHYPVIIDNMMNLELLFWATHVSGD
                     SSFYTIAVTHANTTMRNHYRPDYSSFHVVDYDSVTGAILAKKTAQGYSDSSAWARGQS
                     WGLYGYTMVYRSTHDPKYLEQARNIARFLLTNPNLPADKIPYWDYNAPGIPNTRQTTG
                     IPGPLRDASAAAIMASALIELSHYVQPDEGRQYVDVAEQIIVNLSSNTYKPVVGSNGG
                     FILRHSVGHLPAHSEVDVPLTYSDYYFVEAMLRYRELAQKGK"
     gene            complement(3591..4214)
                     /locus_tag="VKQ52_16020"
     CDS             complement(3591..4214)
                     /locus_tag="VKQ52_16020"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HLZ88764.1"
                     /translation="MEMIKTYRIETERLVIRGYEPADALKLQRSVVRSMAHLRPWIPW
                     AREEPRELEWMVNAVRTFRGRLDLSQDAVYGIFDKAEAEQIGGTGLHNRIGKDAREIG
                     YWIDINHIHKGYATEAVSALLRVAFGIEGYARLEIRCDPANMASRQIPERLGFRHEMT
                     LKNNSTDGDGKPVDTMVWALTRSEYAAAGPGDIVMRAYDFMGKPIEV"
     gene            complement(4205..>4581)
                     /locus_tag="VKQ52_16025"
     CDS             complement(4205..>4581)
                     /locus_tag="VKQ52_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLZ88765.1"
                     /translation="QPIQSVSGGGHWGGGMFINAFDMGRFGLLTLHRGNWNGRQLLSE
                     QWVKQALTPTTANRGYGYMNWFLNTDRKLLPSAPASAFVHIGNGTNFIYVDPEHDLVA
                     VVRWIENGAMDGMVKQILAAWK"
BASE COUNT         1003 a         1633 c          975 g          970 t
ORIGIN      
        1 ttcatcccga tctccacatc gttataattc gtaaactcgt cgcagaataa atatagcttt
       61 tttccgctcc ctgccttact tcttttcttc caccatctct tcaatgtcgt tttgtataac
      121 aacggcatcg accgttccgg ggcaaatccc accagcttct tcacccagtt ttgtctcaca
      181 aataaattat acaccgtggg aaccagcatc cccagctgcg cgcttttcga aaaggacgcg
      241 atcatccggc ttcgcatcgg caccccattg gcatcataat aatgctgcag gaactccgcc
      301 ttcagcttcg ccacatccac attcgacgga cattccgact tacagccctt acagctcaga
      361 cagagatcca tcacatcgta gatctcctta ttgtcatacc ggttgggctt atcactccgg
      421 gtaagaaact cccgcaggat attggcccgg gccctcgtcg tatccttctc gttgcgcgtg
      481 gccataaagg aaggacacat cgtcccaccc gacaaatgcg tcttccggca atccccgctg
      541 ccgttacact gctcggcatg ctgcaggata tcctgcccgt gaaaccggaa aagggtaggg
      601 aaggggggcg tctgctgccc cggctcatac cgcagctgag tgttcatcgg cggcgtatcc
      661 acgatcttgc cgggattaaa gatattcacc gggtcccagc tgcgttttaa ctccttgaac
      721 aactggtaat ttttctcacc caccatctgc gggatgaact cgccccgcaa ccgcccatca
      781 ccatgctcgc cgctaagcga cccctggtat ttcttcacca gcgtggcaat ctcttccgcg
      841 atcgcgcgga aaagccggtt gccctcctcc gtctttagat tgataatggg ccgcaggtgg
      901 atttccccgc tgcccgcatg tgcatagtga acggaataca gtccatattt cttcaggatc
      961 tcattaaaat cccggatata ggccggcaga tcatgtacat ccacagccgt gtcctcgatc
     1021 accggcaccg ccttttcatc acccggcaga ttgctcagca acccaagccc cgcctttcgc
     1081 agcgaccaga tccgccccgt atccgccccg aacaacaagg ggaaatgata cccgagcccg
     1141 gcggcccgca tctccgcctc caccgccgcc gcgatcttcc gtatctcctc ccggtcatcc
     1201 ttcttgaact cgatgacaag gatcgcaccc ggatcaccct gcacaaaaaa gcggttcttg
     1261 cgctgctcga tattatcctt cgtacactcc aggatataat gatcgatcag ctcactggcc
     1321 gtaggcccat atttcaacgc aatgagattg gcccgtaagg actcgtcgat cgtattgaag
     1381 tggacacaga gcagtcccgt ctcctttggc ggcaacggcg agagatccag tttcagttcc
     1441 gtcaggaaag ccagcgtccc ttccgaacct gccagtagcg tacaaaaatt gaagtcggga
     1501 cacccggccg taaaaggcgc cgtatccagc agcaggtcga gggcataacc cgtattccgc
     1561 ctttcgatgc tcttcttcgg gaactgctcc cgtatcccta cctggttgtc gtaattgccg
     1621 agcatccggc gcgtctcccg ataaagcgaa ttctccagac ccgagccctc gcatttggca
     1681 tgaaattcct ccaaccctat ccgcccgaac acaacgtccg tcccatcgct aagcaaggcc
     1741 ttcacctcta ccagatgctc tctcgtgctc cggtacacca ccgaattgct gccgcaggag
     1801 ttattcccca ccatcccccc gatcatcgcc cggttggcgg tagacgtctc cggaccgaat
     1861 aacagtccat gcggcttcag gaacaggttc agatcatccc ggataacccc gggctgtacc
     1921 cgcacccacc gctcctccac attcagctcc agtatcccgg taaaatattt cgaaacatcc
     1981 acgacaatgc ccttccccac aacctgcccg gcaagcgaag tgcccgcggt ccgcgggatc
     2041 agggaggtcc tttccaccct cgcaaaggcg atcagctttc ggatatcttc cacactccgg
     2101 ggaacggcaa ctgccagcgg catctcccgg taagcggaag catccgtggc atacagggtc
     2161 cgcatggtct tgtcataata caactcaccc tccagggaac gacccagtgc ctgcaatttt
     2221 tcatccatga tcgcaaaaat acaccctttc acctattttt gtcccatgta ccgcatcttc
     2281 atcgtcgccg cttgcctcgg actcatcctg ccggaggctc ccgccaatgc ccaaaagatt
     2341 caacaacttc aaccaaaacc cgccctcctg cgcctcatcg acggcaacct ccgccaggcg
     2401 gtgaaccaat ataaagtatt gatgggccgc gtccccgccg accggctccc caaaacctgg
     2461 tacgccaatt cggataaact cgaaacaagc gacgccgact ggtggacaag cggcttctat
     2521 ccgggcaccc tgttctacct ctatgaattc tcccacgaca ccaccctcct ggccgaagcc
     2581 cagcgccgcc tcaccctcct cgaaaaagaa caatacaaca aaggcacgca tgacctcggc
     2641 ttcatgatgt actgcagctt cggcaatgcc ctccggctga tccaacagga actcctgcac
     2701 aaccccgccg ccccatacga cagcatcctc ctcaccagcg cccgctccct gagcacccgc
     2761 ttccatcccg ccgtcggctg tatcaaatcc tgggacagca aaccctggca ctatcctgtc
     2821 atcatcgaca atatgatgaa tctcgaactg ctcttctggg ccacccatgt cagcggcgac
     2881 tcctccttct ataccatcgc cgtcacccat gccaatacaa cgatgcgcaa tcactaccgc
     2941 cccgactata gctcttttca tgtcgtggac tatgattccg tcacgggtgc gatcctcgca
     3001 aaaaagaccg cccagggcta ctccgactcc tccgcctggg cccgcggcca atcctggggc
     3061 ctctatggct ataccatggt ctatcgctcc acccacgacc ccaaatacct ggaacaggca
     3121 agaaatatcg cccgcttcct gctcaccaac cccaacctcc ccgccgacaa gatcccctac
     3181 tgggactata acgcccccgg catccctaat acaaggcaga ccacgggaat ccccggtccc
     3241 ctgagagatg ccagcgccgc cgccatcatg gcctccgccc tcatcgagct cagccactat
     3301 gtccagcccg acgaaggccg gcaatacgtc gacgtcgccg aacagatcat cgtcaacctc
     3361 tcctccaaca cctacaaacc cgtcgtcggc tccaacgggg gcttcatcct ccggcacagc
     3421 gtcggccatc tccccgctca ttccgaagta gatgttcccc tcacctattc ggattactat
     3481 tttgtggaag caatgctccg ctaccgcgag ctggcacaaa aaggcaaatg atcgcctcgc
     3541 ccggccgcaa atttctcccg atccccgccc atccgttgcc tgcgccccct tcaaacctcg
     3601 atcggcttcc ccataaaatc atacgcgcgc atgacgatat cccccggacc cgcggccgca
     3661 tactccgatc tcgtcagtgc ccaaaccatc gtatccaccg gcttcccatc cccatccgta
     3721 ctgttgttct tcaacgtcat ctcatgccgg aaacccaaac gctccggtat ctgcctgctg
     3781 gccatattgg caggatcgca acggatctcc agccgcgcat atccttctat cccgaaggcg
     3841 actctcagca atgcgctcac cgcctccgtg gcatacccct tatggatatg gttgatatcg
     3901 atccagtaac cgatctcccg ggcatccttc ccaatgcggt tatgcaatcc cgttccccct
     3961 atctgctccg cctccgcctt gtcgaagatg ccatacaccg catcctggct caggtccagc
     4021 ctgccgcgaa acgtccgcac cgcattgacc atccattcca gctctctcgg ctcttcccgc
     4081 gcccagggta tccagggccg gagatgggcc atgctccgca cgaccgatcg ctgcagcttc
     4141 agggcatcgg ccggctcata accccgtatc actaatcgct ccgtttcgat ccgataggtt
     4201 ttgatcattt ccatgcggcc agtatttgct ttaccatccc atccatcgcc ccattctcga
     4261 tccaccgcac cacggccaca agatcatgct ccgggtcgac ataaataaaa ttcgttccgt
     4321 tcccgatatg cacaaaagcc gaagcgggtg cgctcggaag caatttccta tcggtattca
     4381 ggaaccagtt catataccca taaccccggt tggccgtcgt cggcgtcagc gcctgcttca
     4441 cccactgctc ggaaagcagc tgcctcccgt tccagttgcc gcgatgcagg gtcaacaacc
     4501 caaacctccc catatcaaaa gcgttaatga acatcccccc gccccaatgg ccgccgccgc
     4561 tgacggactg aataggctga c
//
LOCUS       DATGFG010000851         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_988990, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000851 DATGFG010000000
VERSION     DATGFG010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988990"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VKQ52_16030"
     CDS             complement(<1..409)
                     /locus_tag="VKQ52_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88766.1"
                     /translation="MALYLFYIIPICAAIAFLASLTVFFQPAEQRYLKLFSMFLFVNG
                     MAETFSNYLAVQHVDNTFFTSLVTIIAFTFYLFLLREIIRGKTAKRVLLFLLLIYPLI
                     ALVNILFFQKSGFHTVTYGLGCLLIVGCCIYYFL"
     gene            complement(534..992)
                     /locus_tag="VKQ52_16035"
     CDS             complement(534..992)
                     /locus_tag="VKQ52_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88767.1"
                     /translation="MNTIAKKSVNARNQVSRNEKEVLTSAYKQQRWAENSDRLGKADS
                     LSVWFTVEELENFLATVKANGGNGVRMHFGVFPENYSKPEVAGMQTIVMVGNRSKDGS
                     LENAKELLVNRNGKPEIVALDGPTVCPPFCGSGLGRSTLVVRNNGGMEVI"
     gene            1740..3260
                     /gene="purH"
                     /locus_tag="VKQ52_16040"
     CDS             1740..3260
                     /gene="purH"
                     /locus_tag="VKQ52_16040"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HLZ88768.1"
                     /translation="MNKQIKSALISVFYKDGLAPIVKKLHELGVIIYSTGGTQQFIES
                     QGLPCVPVENLTSYPSILGGRVKTLHPSVFGGILGRRDNPTDMEEMARYHIPEIDLVI
                     VDLYPFEETVASTNDDKAIIEKIDVGGPSMIRGAAKNHKDVVVVASKSEYAWLEKMLT
                     EQKGETTLEQRRMLAARAFEVCAHYDVVIARYFSPSGSDYFLESVPSPKSLRYGENPH
                     QAARFFGQLEGLFDQVNGKELSYNNLVDVDAAVQLIQEFADESRAVFAIIKHTNVCGV
                     AIRSSLKEAWDGALAGDPESAFGGVLAANRPIDKATAEAINEIFFEVLIAPSFDQDAL
                     EILRSRRNRILLMQKKKVSPGQQYRSLLNGVLQQQTDAGNYLEWKEVGGRVATDAEKD
                     DLRFANIVCKHLKSNAIALVKNLQLVGKGCGQTSRVDSVRQAIAKAGQFNFDLHGAVL
                     ASDAFFPFNDSVQLAHAAGVSSFIQPGGSIRDKDSIDYCVENKLVMVITGMRHFKH"
     gene            complement(3299..>4222)
                     /locus_tag="VKQ52_16045"
     CDS             complement(3299..>4222)
                     /locus_tag="VKQ52_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88769.1"
                     /translation="WREGFLDLPLVNYWLLDLRETLTRKFPGLVFRYPVFKFVPTYDI
                     DIAWSFLHKGWQRNTAGALKALFTGRWRQLRHRMDVLAGRRPDPFDAYERLDSLHLYC
                     RLKPYYFFLVAAQNKGVDKNILPSSTGFQQLVQYHGAGYRIGVHPSWQSGDEPRLLKE
                     EIEWLEYMAGQPITRSRQHYIRFTLPHTLRRLLEHGIDQEFSMGYGSINGFRASVASS
                     FYWYDLEKEETTRLRMFPYCFMDANAYYEQGFTAAQAMQQLMNYYHQIRKVNGLMVTI
                     WHNNFLGSDPQFAGWREVYEVFSKEEIFWAS"
BASE COUNT         1002 a          959 c         1251 g         1010 t
ORIGIN      
        1 cgaggaagta gtagatgcag cagccgacga tgagtaagca cccgaggcca taggtcacgg
       61 tgtggaagcc gcttttctgg aagaagagga tattgacgag tgcgatgagg gggtagatga
      121 gcagcaggaa gagcaggacc ctttttgccg tctttcccct gatgatctcg cggagcagga
      181 agagatagaa ggtgaaagcg atgatggtga cgaggctggt aaaaaaggtg ttatcgacgt
      241 gctggacggc caggtagttg gagaaggtct ctgccatgcc gttgacgaag aggaacatgg
      301 agaacagttt aagatagcgt tgttcggcgg gttggaagaa gaccgtcagg ctggccagga
      361 aagcgatcgc tgcgcaaatg ggtattatat agaacaggta aagtgccaaa aatacgaagg
      421 gtttggcagg caagatatag gttattttat agttttcata aataaaagac gcccgtcgga
      481 gacgggcgcc ttttattttg gtatcttttc gagataggtc tataagtctc tgatcagatg
      541 acttccattc cgccgttgtt gcgaacgacg agggtcgatc tgcctaagcc gctgccgcag
      601 aagggggggc aaacggtagg accgtccagt gcaacgatct cgggtttgcc gttgcggttg
      661 acgagaagtt ctttggcatt ttcgagggaa ccgtccttac tccggttgcc gaccattaca
      721 atggtctgca tgcctgccac ttcaggtttg ctgtagttct cgggaaacac accgaaatgc
      781 atacgcacgc cgttgccgcc gttagctttt acggttgcca ggaagttctc gagttcttcc
      841 acggtgaacc atacgctaag cgagtcggct ttacccaggc gatcagaatt ttctgcccac
      901 ctttgttgtt tgtaagcgga agtgagaact tccttttcgt tacgggagac ctgattccgg
      961 gcatttacgg atttttttgc aatagtgttc atagctgact gtttttagaa tgttgaagaa
     1021 taatttgctg ttgtaaatgt attcccaacc ccggcacagc gctttacaca aggaaatata
     1081 aatcacccaa ttataaatta atattatccg taaaataaag gggatcagac aatcaggagc
     1141 ttaatatgac aaaaaaaagg ttttttcggc atataaaaaa cagtaaaata gtcaggtatt
     1201 tgcacgtgaa acacgcagtg aaaagttgcg ctttttacgc aaaaaaaata cgggtcattt
     1261 ttcgaccggc attttcaccg gtttggtttt tttattggac atttaataaa gaggttgcca
     1321 gaacgtcccg tttttaaaca tttgatatcc ttaaaacaag taaacgcctg tgcttttgtc
     1381 cctgctgaac tgcctgaccg acgacgagcc ggtctccaaa aattgacctc aagagtttag
     1441 ttagcaacag catacataac cattaacacc ttatcctgtt tatttctgcg tgtgtgggta
     1501 tgtatgctga tgctccgagc caagatataa acgatatgcg cactgctgtt agtacgcatg
     1561 tgaaaatcga ttacggctta cctctctgtt accattcaaa aaaccggcca atggtcggtt
     1621 ttttgtattt tcgcgcttta aggcgaaaaa aaatgtcgcc ttgcgcgaaa ataggctaat
     1681 tattggcctt cggccggcgg acccgtggtc cgccctgttc gcgccgacgc aataacgtta
     1741 tgaataaaca gataaagtcc gcgctgatct cggttttcta caaggatggt cttgccccta
     1801 tagtaaaaaa actacacgag ctgggggtga ttatttactc gactggtggt acgcaacagt
     1861 tcatcgaatc gcaggggttg ccctgcgtgc cggtggaaaa cctaacgtct tacccctcga
     1921 tcctgggggg gcgggtaaag accctgcacc cttctgtttt tggcggcatc cttggcagga
     1981 gggataaccc caccgacatg gaggagatgg cgcgctatca tattcccgag atcgacctgg
     2041 tgatcgttga cctgtacccg tttgaagaga cggttgcgag cacgaacgat gacaaggcga
     2101 tcattgaaaa gatcgatgtc ggcggtcctt ccatgatccg cggggcggcc aagaatcata
     2161 aagatgtggt ggtggttgcg tccaaatccg aatatgcgtg gctggagaaa atgttgacgg
     2221 agcagaaggg ggaaacgacg ctcgagcagc ggcggatgct cgcggccagg gccttcgagg
     2281 tgtgtgcgca ttatgatgtg gtgatcgcgc gctatttcag tccttccggc agcgattatt
     2341 tcctcgagtc cgtaccgtcg cccaaatcgc ttcgttatgg ggagaacccg catcaggcgg
     2401 cccggttctt tggccagctg gagggacttt tcgaccaggt gaacggcaag gagctctcct
     2461 ataataatct tgtcgatgtg gatgcagcgg tgcagttgat ccaggagttt gccgatgaga
     2521 gcagggcggt gttcgccatc atcaagcata cgaatgtatg tggtgtggcc atccggtctt
     2581 ccctgaagga agcctgggac ggtgcgcttg ccggtgaccc ggagagcgct tttggcgggg
     2641 tattggcggc taaccggccg atcgacaagg ccacggccga agccatcaac gagatctttt
     2701 ttgaagtact tatcgcgccg tccttcgacc aggacgcgct ggagatcctt cgttcgagga
     2761 ggaaccgcat cctgctgatg caaaagaaga aggtatcgcc ggggcagcaa tatcgcagcc
     2821 tgttgaatgg ggtattgcag cagcagacgg atgcgggcaa ttacctggag tggaaggaag
     2881 tgggtggccg ggtggcgacc gatgcggaga aggacgacct gcgctttgcg aatatcgtct
     2941 gcaagcatct gaagtcgaat gcgattgccc ttgtcaagaa cctgcagctg gtgggtaagg
     3001 gatgcgggca gacgagccgg gtcgactctg tgcggcaggc cattgccaag gcggggcagt
     3061 ttaatttcga tctgcatggg gccgtgctgg cgagcgatgc gttctttccc tttaacgatt
     3121 cggtgcagct ggcgcatgcg gccggcgtga gctcgttcat tcaacccggg ggttccatcc
     3181 gggataagga ttccatcgac tattgtgtgg agaataaact ggtgatggtg atcacgggca
     3241 tgcggcattt caagcactaa aagggcggag ccccggatca ggaggggctg aggctgggtc
     3301 aggaggccca gaagatctct tcttttgaaa atacttcata cacttcccgc cagccggcga
     3361 actggggatc gctgcccagg aagttattat gccagatggt gaccatcagg ccgttgacct
     3421 tgcggatctg atggtaatag ttcatgagtt gctgcatggc ctgtgcggcg gtgaatccct
     3481 gttcatagta ggcattggca tccatgaagc agtaggggaa catgcgcagt cgtgtcgttt
     3541 cttccttttc caggtcatac cagtagaaag acgaggccac cgaggcccgg aagccgttga
     3601 tgcttccata gcccatggag aactcctggt cgatgccgtg ttccagcagg cggcgcaggg
     3661 tatgcgggag cgtgaaacgg atataatgct gacggctgcg ggtgatgggt tggccggcca
     3721 tgtattcgag ccattcgatc tcttctttta gcagccgggg ctcatccccg ctttgccagg
     3781 acggatgaac gccgatgcga tagccggcgc cgtgatattg caccagttgc tggaatccgg
     3841 tggaggatgg gaggatattc ttatcgacgc ctttgttttg tgctgctacg aggaagaaat
     3901 agtagggttt caaccggcag tagagatgca gtgaatccag cctttcatag gcgtcgaagg
     3961 gatcggggcg tcgtcccgcg aggacatcca tgcgatggcg gagctgccgc cagcgaccgg
     4021 tgaaaagcgc tttcagggcc ccggcggtat tgcgttgcca tcccttatgc agaaaggacc
     4081 aggcgatgtc gatatcatag gtggggacga atttaaaaac cgggtagcgg aagacgagac
     4141 cgggaaattt ccgggtgagg gtttccctga ggtcgaggag ccagtaattt acgaggggga
     4201 gatcgagaaa cccttcgcgc ca
//
LOCUS       DATGFG010000852         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_385879, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000852 DATGFG010000000
VERSION     DATGFG010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385879"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..170)
                     /locus_tag="VKQ52_16050"
     tRNA            complement(97..170)
                     /locus_tag="VKQ52_16050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(134..136),aa:Pro,seq:tgg)
     gene            complement(288..2474)
                     /locus_tag="VKQ52_16055"
     CDS             complement(288..2474)
                     /locus_tag="VKQ52_16055"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HLZ88770.1"
                     /translation="MILNRLAAALFFSLLLSSLSVTAQKKNIGFELHIHRATSPIHID
                     GSIDEPAWQQAERAGNFYMVLPMDTSHAVVRTEVRMTYDEKYLYLVAECFLPGPGRDM
                     VESLRRDFSFVKNDNFIFFIDPFEDQTSGFTFGANAAGGQWDGLMYEGGKVDLSWDNK
                     WSSVVRRYSDRWVLELAIPFKTLRYKNGLTHWGVNFSRNDLKTTEKSAWAPVPRQFPT
                     ASLAYTGVLVWDVPPPPPGPNISVIPYVLGGVQKDYTVPGGNVYRKEIGGDAKVAITS
                     SLNLDLTVNPDFSQVDVDKEVIDLSRYELFYPEKRQFFLENADHFNNLGYPTIRPFFS
                     RRIGLGIPIEFGTRLSGKLDPNWRIGLMDMQTNKVDSIGLPEQNFSVLALQRKIFSRS
                     NIGFIFINKQSIHYQPGKDSSLPVYSQYNRNMGLEFNLASSDNYWTGKILLLKAFSPG
                     KRGDDYVHAGNLQYLNRHWLIGAEYEYTGGNYSAEVGYVPRTGYFKINPQVNYTFLPS
                     GGVVLSHGPQLTSTYYYNEAFHRTDNETVFTWLTTFRNRSTLSGVLMNDYVQLLSPFD
                     PTNTGVAFLVTGTRHRWNTAGVDYVSKPQALFTYDLSLRYGGYYADGNKFTLVNDVGY
                     RIQPYVNFTMSTTYTNLILPQPWGHTHFWLIGPKLDITMTNTLFLTTYIQYNQQTRNM
                     NLNTRLQWRYKPASDLFLVYTDNYLTTPFSVRNRAFVLKFNYWWNL"
     gene            2528..>2852
                     /locus_tag="VKQ52_16060"
     CDS             2528..>2852
                     /locus_tag="VKQ52_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88771.1"
                     /translation="MKQPKIPFEQLPITSYLLRWTLLVIPVSLTAGSLVALFLWLLDK
                     ATQFRFAHSWLLFLLPLAGVLIYLLYKYLGKNSGLGNNLIIDEIHAPGGGVPARMGPL
                     VLLTTV"
BASE COUNT          652 a          779 c          785 g          636 t
ORIGIN      
        1 cgggacggtt ttgttttccc gtccgctttg gaaagcaagc tttccccgct cccggaaaaa
       61 caaaacccgc agctatcgct gcgggctcct tgttcgtcgg gaagacagga ttcgaacctg
      121 cgaccccctg gtcccaaacc aggtgcgcta ccggcctgcg ctacttcccg atccggggga
      181 atccggtgca tccggcggat ctggtgatcc cgggaaattg gcgatcccgg gaaaaccggc
      241 gaatccggcg aatgaggtgc aaagctaggg atttataggc gaatgtccta gagattccac
      301 caatagttga attttaggac aaaggcgcgg ttgcggacgc tgaagggggt ggtgagatag
      361 ttatcggtgt agaccaggaa gaggtcggag gcgggcttgt accgccactg gagacgcgta
      421 ttgaggttca tgttgcgggt ctgctggttg tactggatat acgtcgtaag gaagagcgtg
      481 ttggtcatcg taatgtcgag tttagggccg atgagccaga aatgggtatg gccccagggc
      541 tggggaagga tcaggttcgt atacgtggtg ctcatagtga aattgacata gggctggatc
      601 cgatagccca catcgttgac gagggtaaat ttattcccat cggcataata gccgccataa
      661 cgcagcgata gatcataggt aaagagggcc tggggcttgc tgacgtaatc gacgccggcg
      721 gtattccacc tgtgacgcgt gccggtaacc aggaacgcaa caccggtatt tgtggggtca
      781 aagggcgaaa gcaactgcac atagtcattc atcagcaccc ccgacagcgt actcctgttg
      841 cggaacgtgg tcagccaggt gaagaccgtc tcgttgtcgg tgcgatgaaa ggcctcatta
      901 tagtaatacg tagaggtcag ctggggccca tgactcagca ctaccccacc cgacggcaaa
      961 aaggtgtagt tcacctgcgg attgatcttg aaatagccgg tcctcggtac ataccccacc
     1021 tccgcactat agttgccccc ggtgtactcg tactccgcgc cgatgagcca gtgcctgttc
     1081 aaatactgca gattgcccgc gtgcacatag tcatccccgc gtttaccggg gctaaacgcc
     1141 tttaacagca gtatcttacc cgtccagtaa ttgtccgatg aagccaggtt gaactcgagc
     1201 cccatattcc ggttgtactg cgaatacaca ggcagactgc tgtccttgcc cggctgataa
     1261 tggatggatt gcttatttat aaaaatgaag ccgatattgc ttcgggaaaa gatcttccgc
     1321 tgcaacgcca gcaccgagaa gttctgctcc ggcaacccga tggaatccac cttattcgtc
     1381 tgcatgtcca tcagcccgat ccgccaatta gggtccagct tcccgcttag ccgcgtcccg
     1441 aattcgatgg gaatgcccag gccgatgcgc cgggaaaaga acggccggat cgtcggatat
     1501 cccaggttgt tgaaatgatc cgcattctcc aaaaagaact gccgtttctc gggatagaac
     1561 agctcgtaac ggctgagatc gatcacttcc ttgtcgacat ccacctgcga gaaatcgggg
     1621 ttcaccgtca gatcaaggtt gagggaggaa gtgatcgcca cttttgcgtc gccaccgatc
     1681 tctttcctat agacattccc gcccggaacg gtatagtcct tctgtacccc gcccaacaca
     1741 taggggataa cggaaatatt gggacccggc gggggcggcg gcacatccca caccagcacg
     1801 ccggtataag ccagggaagc cgtgggaaac tgccgcggga cgggcgccca ggcggatttc
     1861 tcggtcgtct tgagatcgtt gcggctgaag ttgacgcccc agtgggtcaa tccattctta
     1921 taccgcaggg tcttgaaagg aatggcaagc tccaggaccc agcgatcgct gtatcgcctg
     1981 accaccgaac tccatttgtt atcccagctc aggtcgacct tccccccttc atacatcagg
     2041 ccatcccatt gaccacccgc ggcattggcg ccgaacgtga agccgctggt ctgatcttcg
     2101 aacggatcga taaagaatat gaagttgtca ttcttcacaa agctgaagtc gcgccggagc
     2161 gattccacca tgtcgcgacc aggaccaggc aggaaacatt cggcgacgag gtacaggtat
     2221 ttctcatcat aggtcatccg cacttcggta cggacaacgg catgactggt gtccatcggc
     2281 agcaccatat agaaattccc cgcccgctcg gcctgctgcc aggccggctc atcgatggat
     2341 ccgtcgatat ggatgggact tgtcgcccgg tgaatatgca actcgaagcc gatattcttt
     2401 ttttgcgcgg taaccgaaag agaggaaagg agcagcgaaa aaaacagggc agcagccaat
     2461 cgattaagga tcattgataa aaaatacaat tttttaccga tattgaccaa atccataatt
     2521 tgtaggcatg aagcaaccca agatcccgtt tgagcagctc ccgatcactt cctatcttct
     2581 tcgctggacc cttcttgtca tccccgtttc gctgacggca ggatcgctgg tcgccctgtt
     2641 cctttggctg ctggacaaag ccactcaatt ccggtttgcc cattcctggc tgctcttcct
     2701 tcttccgctt gccggcgtcc tgatctacct gttgtataaa tacctgggta agaattcggg
     2761 gttgggcaat aacctgatca tcgacgagat ccacgctccg ggtggaggtg tgccggcgcg
     2821 gatgggtccg ctggtgctgc tgacgacggt ga
//
LOCUS       DATGFG010000853         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_492410, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000853 DATGFG010000000
VERSION     DATGFG010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492410"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VKQ52_16065"
     CDS             complement(<1..833)
                     /locus_tag="VKQ52_16065"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88772.1"
                     /translation="MIFAELKDIVRNDSRLRLLLLASLLVQVGICLTAVGIYHPDQYF
                     QIIEFSSYQLHRPSAAGHVWEFAAHLRPTIQVYLFSAYYSVCTLLGIHDPYGQLTLLR
                     LLFGLALFVLFNVICLYYFRDDRRVLFLVLLILNFSWILPYTRTLYSSEMLSSFLFFG
                     ALFLYDVARGGNGRDRWIALLTGFLFSLAFYARFQTAFAMVGFLLWMLWMRGERRLLW
                     PLAAGFVVGVVLNTGLDAGFYHQLVFTPYTYYKVNIVDGVAASMGTASFLLYVGVLAA
                     VV"
     gene            1033..>2206
                     /locus_tag="VKQ52_16070"
     CDS             1033..>2206
                     /locus_tag="VKQ52_16070"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88773.1"
                     /translation="MMRKYPVFALLLALPSFLSAQISYELNTGWKCQAIGKIHATGEK
                     LSQSGYSTSGWLEATVPGTVLTTLINNHLEPDPFYGLNNEKTPDIYTTGRDRYTYWFV
                     KNFKETVPTGNQQVWLDLRGVNYSCEIFLNGQKLNEHTHYGMYLRQSYNITKALAHNG
                     ENRLAILVYPPDPVGNPNGGQGGDGEIARNVTHQYVAGWDWIQPIRDRNTGIWDKVTI
                     HHTGGTVRIRNPHIITEVSGVRYPDIAARQQAPAVLRVSAELENTGADTVTGKLSYIL
                     DGATTTATVRLAPGITNEIRLPFHIIEHPRLWWPNGYGTQELYHTQLTFTGSDAIPLD
                     FGVRELATSWNSTTRSRQLTVNGQKIFIKGGNYIVSDELLRLSPERYDAEVRFHRDM"
BASE COUNT          599 a          768 c          479 g          360 t
ORIGIN      
        1 accacagccg ccagtacacc cacatacaac aggaacgaag ccgtccccat cgacgccgcc
       61 acgccatcca caatgttgac cttataatac gtatacggcg taaacaccaa ctgatgataa
      121 aaacccgcgt ccagcccagt atttaaaacc acccccacaa caaaccctgc tgccagcggc
      181 cacaaaagcc tccgttcccc ccgcatccac aacatccaca gaagaaaacc aaccatcgca
      241 aacgccgtct gaaaccgggc ataaaaagcg aggctgaaaa gaaaacccgt caacaatgcg
      301 atccacctgt ccctgccatt gcccccgcgg gccacatcat acaggaacaa ggccccgaaa
      361 aacaaaaagg aggagagcat ctcgctgcta tagagcgtcc gcgtatacgg caggatccac
      421 gaaaaattaa ggatcagcaa caccaggaac aagacccgcc ggtcgtcccg gaaataatac
      481 aggcagatca cattgaacaa cacaaaaagc gccagcccga agagcaaccg caacaaggtt
      541 agctgcccgt acgggtcatg gatgccaagc agggtacaaa ccgaataata ggcggaaaaa
      601 agatagacct gtatcgtcgg ccggagatgg gccgcgaatt cccacacatg ccccgcggcc
      661 gagggccggt gcagctggta ggaggagaac tcgatgatct ggaaatactg gtcgggatga
      721 tagatgccca cagccgtcag gcaaatgccc acctggacaa gaagggaggc cagcaacaat
      781 agccgcaacc ggctatcgtt ccggacgatg tctttcaatt cggcaaaaat catagatcag
      841 gtcagcagtt gattcgatgc gataaccgaa aataagccgc ttttgtttaa cccgccccat
      901 taacttttcc cccgcggccc cccacccagc aaaactcctc tcgcccgcta ccaccgcctc
      961 aaaaactccg ccatccagcc ccactccatt aaattccttt gtgctaaaca aatcaacctg
     1021 tagttttacg ccatgatgag aaaatacccc gtattcgccc tcctcctggc cctgccctcc
     1081 ttcctctccg ctcaaatctc ctacgagctg aacaccggct ggaaatgcca ggccatcggc
     1141 aagatccacg ccaccggcga aaaactttct caatccggtt actccaccag cggctggctg
     1201 gaagctaccg taccgggcac cgtcctgaca acacttatca acaaccacct cgaacccgac
     1261 cccttctatg gcctgaacaa cgaaaagacc cccgatatct acaccaccgg ccgcgatcgc
     1321 tatacctact ggttcgtaaa gaacttcaaa gagaccgtcc ccaccgggaa ccaacaggtc
     1381 tggctcgatc tgcgcggagt aaactatagc tgcgagatct tcctcaacgg ccaaaagctg
     1441 aacgaacaca cccactatgg aatgtacctg cggcaatcct ataatattac aaaggccctc
     1501 gcccataacg gcgagaaccg cctcgccatc ctcgtctatc cccccgaccc cgtcggcaac
     1561 ccaaacggcg gccagggcgg cgacggcgaa atagcccgta acgtaaccca tcaatacgta
     1621 gccggctggg actggatcca gcccatccgc gaccgtaaca ccggcatctg ggataaggtc
     1681 accatccacc ataccggcgg aacggtccgc atccgtaatc cccatatcat caccgaggtc
     1741 tccggcgtcc gttaccccga catcgcggcc cggcaacagg cccccgccgt tctccgcgtc
     1801 agcgccgaac tcgagaacac cggcgccgac accgtcacgg gcaaactatc ctacatcctc
     1861 gatggcgcca ccaccactgc caccgtcagg ctggcgcccg gcatcaccaa tgagatcaga
     1921 ctgccctttc atatcattga acatccccgt ctctggtggc caaatggcta tgggacgcag
     1981 gaactctatc acacgcagct caccttcacc ggctccgacg ccatccccct cgacttcggc
     2041 gtccgcgaac tcgccacctc ctggaattcg actacccgta gccggcagtt gaccgtcaac
     2101 ggccagaaga tcttcataaa aggtggcaac tacatcgtct ccgacgaact cctccgcctc
     2161 agccccgaac gctatgacgc cgaggtccgt ttccaccgcg atatga
//
LOCUS       DATGFG010000854         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_973691, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000854 DATGFG010000000
VERSION     DATGFG010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973691"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VKQ52_16075"
     CDS             complement(<1..644)
                     /locus_tag="VKQ52_16075"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLZ88774.1"
                     /translation="MSLIVVGSMAYDAIETPFGKTDWIVGGAATYVAYAASNFVKPIN
                     QVSIVGYDFAPEELDQLQKRGVNLEGVEIKKDKKSFFWSGRYHMDMNTRDTLVTDLNA
                     FMDFNPVIPESYQGSEFLMLGNITPGLQISVVQQMKRRPKLIAMDTMNFWMDNALDDL
                     KKLLKLVDVLMVNDSEARQLSGEFSLVKAARAIMKMGPRFVIIKKGEHGALLFHG"
     gene            716..1306
                     /locus_tag="VKQ52_16080"
     CDS             716..1306
                     /locus_tag="VKQ52_16080"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ88775.1"
                     /translation="MAKIQSKRNSTKKEVIIEKASRLFREKGFGAASMRDLAEHVGVE
                     AASLYNHIQSKSEILQTICFKVANEFLTNLEQVEVSPQPTLRKMEAIIRLHIRMMLEQ
                     YEYVYIADHEWRHLPEPYLSNFLNQRRNYRKRLSDIIEGGIAKGEVKAIDPYTAVLVI
                     LSAISGIDSWQRSRKSTNAETLESNMVKYLIEGLKK"
     gene            1303..>2001
                     /locus_tag="VKQ52_16085"
     CDS             1303..>2001
                     /locus_tag="VKQ52_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLZ88776.1"
                     /translation="MSHFRKLIVEDIRRETADCVSIAFAIPAPLREEFSYHQGQNITI
                     RARIGDTEIRRSYSICSSPLEEELRIAVKRVPGGVFSTFANEQLQKGQELEVLPPSGK
                     FYTELRAGNRKNYLAFAAGSGITPVISLIKTTLSVEPDSHFTLVYGNRHRPSIIFREQ
                     LEALKDRHMHRLALHHILSREQMDVPLNQGRIDAAKCAELCPRLIDLSSMDEVYLCGP
                     QEMIFTVSDWLEQQG"
BASE COUNT          450 a          518 c          563 g          470 t
ORIGIN      
        1 ccgtggaaga gcagcgcgcc gtgttcgcct ttctttatga tcacgaaccg ggggcccatc
       61 ttcataatgg cgcgggcggc ctttacgagg gagaattctc cgctcagctg acgggcttcg
      121 ctgtcgttta ccatcagcac atcgaccagt ttcaataatt tcttaaggtc gtcgagcgcg
      181 ttgtccatcc agaagttcat cgtgtccatg gcgatgagct ttgggcgccg tttcatctgc
      241 tggacgacgc tgatctgcag accgggggtg atattaccca gcatcaggaa ctcgcttccc
      301 tggtagcttt ccgggatcac ggggttgaag tccataaacg cgttcaggtc ggtgacaagg
      361 gtatcccggg tattcatgtc catatggtat ctgccactcc agaagaagga tttcttgtcc
      421 tttttaattt cgacgccttc caggttgacc ccgcgttttt gcagctggtc cagttcttcc
      481 ggggcaaagt cataacccac gatggatacc tggttgatgg gttttacaaa attgctggct
      541 gcgtatgcga cataggtggc cgctccgcct acgatccaat ctgtctttcc gaaaggagtt
      601 tcgatggcgt cataggccat ggaacctact acaattagtg acatttactt caattttggg
      661 cgcaaaatag gcaataattg cactaacagt tgttagttct ttttatattt gtcccatggc
      721 caagatccag agtaagcgga acagcaccaa gaaggaggtg atcatcgaaa aggcttcgcg
      781 tcttttcagg gaaaaggggt ttggggccgc atcgatgcgg gacctggccg agcatgtggg
      841 ggtggaagcc gcctccctgt ataaccatat ccagtccaaa tcggagatcc tgcagaccat
      901 ctgtttcaag gtggccaatg aattcctgac caacctggag caggtggaag tcagtccgca
      961 gccgacgttg cggaagatgg aggcgatcat ccggctgcat atccggatga tgctggagca
     1021 gtatgaatat gtttatatcg ccgaccatga atggcgtcat ctgccggagc cctatctctc
     1081 caatttcctc aaccagcggc gcaattaccg caagcggctg agcgatatca tcgagggggg
     1141 tatcgccaag ggcgaggtaa aggcgatcga tccctatacg gccgtcctgg tgatcctctc
     1201 cgctatcagc ggtatcgaca gctggcaacg gagccgcaag agtaccaatg ccgagaccct
     1261 tgagtcgaat atggtaaaat atcttattga aggattgaag aaatgagcca tttcaggaaa
     1321 ctaatagtcg aagatatccg cagggaaacg gccgattgtg tctcgattgc ttttgccatc
     1381 ccagccccgt tgcgggaaga attcagctat caccagggcc agaatatcac gatccgggcc
     1441 cggatcgggg acaccgagat ccggcggagc tattccatct gcagcagtcc gctggaggag
     1501 gaactgcgca tcgccgtcaa gcgggtgccg ggcggggtct tttcgacctt tgccaatgaa
     1561 cagctgcaaa aaggccagga gctggaagtg ttgccgccga gcgggaaatt ctataccgaa
     1621 ttgcgggcgg gtaaccggaa gaattatctt gcctttgccg cgggcagtgg catcaccccg
     1681 gtgatctcgc ttataaagac cacgctttcc gtggagccgg acagccattt tacccttgta
     1741 tatggcaacc ggcatcgccc ttcgatcatt ttcagggagc agttggaggc cctgaaggac
     1801 cgccatatgc accggcttgc gctccatcat attcttagcc gggagcagat ggatgttccg
     1861 ctcaaccagg gcaggatcga tgcggcaaaa tgcgccgagc tctgtccccg gcttatcgac
     1921 ctgtcctcga tggacgaggt gtacctctgc ggtccgcagg aaatgatctt taccgttagt
     1981 gactggctgg agcagcaggg g
//
LOCUS       DATGFG010000855         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_611848, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000855 DATGFG010000000
VERSION     DATGFG010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611848"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VKQ52_16090"
     CDS             complement(<1..413)
                     /locus_tag="VKQ52_16090"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HLZ88777.1"
                     /translation="MRPAGNLLGVVLSGGESRRMGRDKGLLDTGSGAWVLRMGEKLAQ
                     QQLPVIYSINKEQFPAYSAILPADRLVVDAGEREGPLNGLFSVHRGFPRHDLLLVACD
                     MQDLDAAALTGIIAAWRIEEADAYAYEVEGVLQPF"
     gene            complement(397..966)
                     /locus_tag="VKQ52_16095"
     CDS             complement(397..966)
                     /locus_tag="VKQ52_16095"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HLZ88778.1"
                     /translation="MTPSSSYPPAAEDRGMAAPSHPSGPAAASPGQAPAPEALSSEPL
                     DIVGLLGGAHHPGAGAVVLFSGEVRDNNQGRAVSSLEYEAYAPLATSMIAEILATARQ
                     RWSLQTAVAQHRTGRVAVGDTAVVVITASAHRSEAYAANRYIIDRIKHEAPIWKCEHY
                     ADGTKEWGNNCNCHTVTGDVNKHIYETGG"
     gene            complement(926..1159)
                     /locus_tag="VKQ52_16100"
     CDS             complement(926..1159)
                     /locus_tag="VKQ52_16100"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HLZ88779.1"
                     /translation="MKIHVYAGLKDYFQPEFEVSGLVRNTDELKALLLTINDKAGAIL
                     SSCRFAVEEGFIDNAFKLNENDTIVIIPPSSGG"
     gene            complement(1185..2141)
                     /locus_tag="VKQ52_16105"
     CDS             complement(1185..2141)
                     /locus_tag="VKQ52_16105"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HLZ88780.1"
                     /translation="MPVIQDRYGRTFRTLRVSLLQHCNLGCVYCVAGDEEVKQANATH
                     GSLPVEELLAIIRRLREQLPLDTVRLTGGEPLLYPALLPLITGIRAAGISAVRLTTNG
                     FLLERLAPALKEAGLDAMNVSLDAIDEEVFFRMSRRHSVTRIIKGIDAAVAAGLKIKI
                     NSVMMKGMNDSQLLPLLDFAFSRGLTIRFLEVMAMGHLHAEAGRYLVTQEEILQTIGN
                     HYTLTPEPRSESATARYWQTEEGHRFGIIANESEPFCRDCDRLRLDSSGHIYGCLSSN
                     LPITLGLAETAEDWNRKLEEALAQKQAVRFVGSDLSMLHIGG"
     gene            complement(2219..>3335)
                     /locus_tag="VKQ52_16110"
     CDS             complement(2219..>3335)
                     /locus_tag="VKQ52_16110"
                     /inference="COORDINATES: protein motif:HMM:NF012522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HLZ88781.1"
                     /translation="LNFISSAVNNHWHLVVRRPGTNRLWSWKTGIYSPDIPRIGRLVE
                     ESLAAMKALGDEEWGQRLYETVQSRGGFPEQPLLPELRLPDFRLPYYEGFNRSGNKNW
                     LGVYRREELFPLAFLKEVCLIALQTKVGQLYTTPWKSLLIKDIEDADVHQWEYILGKY
                     RMNVRHASNELNWQIEDLCPDGLRLKRYLVRLLDQDDIRTEGLSFSIKTKQGSGLPGS
                     VIIRKQEGIRPDQLKLLDRYSLSHTPDFNPHSREVVLYRKDLEKDNLFPYIVSLCKEF
                     YRQQSERHSPLPSHPAAVDPGKGAAASGGWECPHCLTVYHEQYGDEEQGIAPGTPFAK
                     VTPDYVCSVCGGAKADFRALEKSAAGRTVGSPAEGQL"
BASE COUNT          638 a         1003 c          977 g          717 t
ORIGIN      
        1 cagaaaggct gaagaacgcc ttctacctcg taagcatagg cgtccgcctc ttctatgcgc
       61 caggccgcga tgatccccgt cagcgcagcc gcatcgaggt cctgcatgtc acaggctacg
      121 agcagcaggt catgccgggg gaaaccccgg tgtacgctga agagcccgtt cagcgggcct
      181 tcccgttcgc cggcatcgac gaccagcctg tccgcgggaa gaatcgcgga ataggcgggg
      241 aactgttctt tgttgatgga atagatgacg gggagttgtt gttgggcgag cttttccccc
      301 atccggagca cccaggctcc actgccggtg tcgagcagtc ccttgtcgcg acccatccgc
      361 cggctttcgc cgccgctgag cacgacgccc agcaggttac ccgccggtct catagatatg
      421 cttgttgaca tcaccggtga cggtatggca attgcagttg ttgccccatt cctttgtccc
      481 atccgcatag tgttcgcatt tccagatggg cgcttcatgt tttatgcgat cgatgatata
      541 ccggttggcc gcataggcct cgctgcgatg ggcggaggcc gtgatcacga cgacggccgt
      601 gtcgccgacg gccaccctgc cggtgcggtg ttgggcgacc gctgtctgca gggaccaacg
      661 ttgcctggcc gtggccagga tctccgcgat catcgaagtc gccaggggtg cataggcctc
      721 atattccagg gaggataccg cccggccctg gttattgtcg cgcacctctc cgctgaagag
      781 tacgacggcg ccggcgcccg ggtgatgggc cccgccaagc aggccgacga tgtcaagcgg
      841 ctcgctgctg agggcctcag gcgccggggc ctgtccgggc gaggccgccg ccgggccgga
      901 tgggtgcgac ggcgccgcca ttcccctatc ctccgctgct ggggggtatg atgacgatgg
      961 tgtcattttc atttaattta aacgcattgt cgatgaaacc ttcttccacg gcaaagcggc
     1021 aggaggaaag aatggccccg gccttatcgt taatagtcag caacagcgct tttagttcgt
     1081 cggtattccg aacgagcccg ctgacttcga attccggttg aaagtaatct ttcagcccgg
     1141 catatacgtg aattttcatt gtcgatcctc ccggagtgtt ttccttatcc gccaatgtgc
     1201 aacatactca gatcgctccc tacaaaacgc accgcctgct tttgcgccag cgcctcttcc
     1261 agtttccggt tccagtcctc cgccgtctcc gccagcccaa gcgttatcgg cagattactg
     1321 ctcaggcacc catagatatg accgctgctg tcgagccgga gccggtcaca gtcgcggcaa
     1381 aagggttcgc tttcattggc aatgatccca aaacggtgtc cctcttccgt ctgccagtac
     1441 cgggcggtgg ccgattccga cctgggttcc ggtgtcagcg tataatggtt tccgatagtt
     1501 tgtagtattt cctcctgtgt caccaggtaa cggccggcct cggcatgcag atgccccatg
     1561 gccatcactt cgagaaaccg gatggtaagc ccgcgggaga aagcaaaatc cagcaggggc
     1621 agcagttggg agtcattcat tcccttcatc atcaccgaat tgatctttat cttcaatccc
     1681 gcggctaccg cggcgtcgat gccctttatg atccttgtca ccgaatgacg tctgctcatc
     1741 cggaagaaca cctcttcgtc gatggcatcc agcgacacat tcatggcgtc aaggcccgcc
     1801 tccttcaacg ccggcgccag cctttccagc aggaaaccat tggtcgtcag ccggacggcg
     1861 gagatccccg cggcgcggat acccgttatc aggggcagca gggccgggta caacaggggc
     1921 tcgccacccg tcagccggac cgtgtcgagc ggcaactgtt cccggaggcg ccggatgatc
     1981 gccaacaatt cttcgaccgg caggctccca tgggtggcat tggcctgctt tacttcctca
     2041 tcccctgcga cgcaatagac acagccaagg ttacaatgtt gcagtaaact gacccgcagg
     2101 gtccggaagg ttcgtccata tcgatcttgt atcactggca tcagaccata aaggtacgat
     2161 tgtcattcgt gaaccgggac atggttttcc gaaaaataaa ccatgcccgg ttcaccgctt
     2221 ataattggcc ttcggccggc gaacctacgg tccgcccggc ggcggatttt tcaagggccc
     2281 ggaaatcggc ttttgcgccg ccgcaaacgc tgcagacata gtccggtgtc acctttgcaa
     2341 aaggggtgcc gggggcgatc ccctgctcct cgtcgccgta ttgttcgtgg tacaccgtca
     2401 ggcagtgcgg gcattcccag ccgccggagg ccgctgcgcc cttgcccgga tccacggcag
     2461 ccggatggga agggaggggc gaatgccgtt cgctttgctg ccggtagaat tccttgcaga
     2521 gagatacgat ataagggaaa agattgtctt tttccaggtc cttgcggtag agcaccacct
     2581 ccctggaatg ggggttgaag tcgggggtat gcgagaggga ataccggtcc agcagtttca
     2641 gctggtcggg ccggatgcct tcctgtttgc ggatgatcac cgacccgggc aggccggaac
     2701 cctgttttgt cttgatcgag aagctcagcc cttcggtgcg gatatcatcc tggtccagga
     2761 gccggacaag atagcgcttc agccgcagcc catccggaca gaggtcttcg atctgccagt
     2821 tcagttcatt ggaggcatga cggacattca tccggtactt gcccaggata tattcccatt
     2881 ggtggacatc ggcatcctcg atatccttga tcagcaggga tttccagggc gtcgtataca
     2941 attgccccac ctttgtctgg agggcgatga gacagacttc cttgagaaaa gccaggggga
     3001 agagctcctc ccggcgatag acgcccagcc agttcttatt gccgctgcga ttgaaccctt
     3061 catagtaggg caggcggaaa tccggcaaac gcagctcggg taacagcggc tgttcgggga
     3121 aaccgccgcg ggattgcacg gtctcgtata accgctgtcc ccattcctcg tccccgaggg
     3181 ccttcatcgc cgcgagcgat tcttcgacaa gccggccgat gcgggggata tcgggcgagt
     3241 agatccccgt tttccaggac cagagccggt tggtgcccgg gcggcgcacc acgagatgcc
     3301 agtgattgtt gacggcgctg ctgataaaat tgaga
//
LOCUS       DATGFG010000856        12453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_958611, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000856 DATGFG010000000
VERSION     DATGFG010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958611"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VKQ52_16115"
     CDS             complement(<1..212)
                     /locus_tag="VKQ52_16115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HLZ88782.1"
                     /translation="MQNAHDRPIRVLVGKVGLDGHDRGARVIATALRDAGMEVIYTGL
                     RQTPEMVVNAALQEDVDAIGISILSGA"
     gene            complement(242..1909)
                     /gene="gltX"
                     /locus_tag="VKQ52_16120"
     CDS             complement(242..1909)
                     /gene="gltX"
                     /locus_tag="VKQ52_16120"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLZ88783.1"
                     /translation="MRFAPSPTGGLHLGGVRTVLYNYLFARRHGGDFILRIEDTDQAR
                     YVPGAEEYIIDSLRWCGLEPDEGPHKGGAYGPYRQSERKALGIYGKYAQQLVDAGHAY
                     YAFDTPGELEAMREKYKTADNPSPQYNHTLRKKMRNPLSLEPAETKRLLAEGTPYVIR
                     IRLEPDQEVRFTDLIRGEVHFSTTGVDDKVLLKADGMPTYHFAVVVDDHLMAITHAFR
                     GEEWLPSAPVHILLWRYLFGEDKMPQWAHLPLILKPDGNGKLSKRDGDRLGFPVFAMN
                     WTDPRTNEPATGFREAGFLPQAFVNLLALLGWNDGSGQEIFTLSELVEKFSLVRVHKG
                     GAKFDFEKAKWFNHEWIKQLPASAYAPQVAPLFAAAGITIPPADLLRPADPEPDYLPP
                     VIPLIPDTLTDISGTPLIDLAALESGLEPAPPPPNRFERVLDLVKDRCALLPEFVPQA
                     AFFFQPPATIDLEAVKPKWTDAKALFFAEYIRQFELATDADAHALETQFKEMAAAAAI
                     KPGELLMPLRVMLVGGKFGPHVFDIAVLLGKEETIRRIRHTLALLKG"
     gene            2166..3113
                     /locus_tag="VKQ52_16125"
     CDS             2166..3113
                     /locus_tag="VKQ52_16125"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HLZ88784.1"
                     /translation="MKISLTFCLLFSLLSASAQLTYETLFVDYDSAWQYKNLKIIPIR
                     RKAGAGMAMQHGDIVPLGQALSTGQVTVTERGTTSFENVHWLRFNTHGKKPVYIAGGE
                     IIAGGRQDRMIVRDTILQPMDKDQYVPVMCVEEGRWSDKEKKFGYGNFANPHLRKVLD
                     STGNQVSIWLEVDRQLQAGAFKNKTFSYLSRYEDKKYLAAQDDYFRFFMNKFAKTDSN
                     VVGFVAITGDKIIGTDIFDGTDLFYPQLDPLLHGYIDDVIASGLPVNIKEPPVKRYMD
                     RLLKDEKSQEEFVKDKGKVFRQNGRVIHVNTYTKETPVQ"
     gene            3134..3763
                     /locus_tag="VKQ52_16130"
     CDS             3134..3763
                     /locus_tag="VKQ52_16130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLZ88785.1"
                     /translation="MFYLVRSPWWLKRFYSRLVWDIPAKDKVLYLTFDDGPHPVATPF
                     VLDELKKHDARATFFCIGKNVQEYPQIYRRVLMEGHRVGNHTQNHLNGWKTDDKRYLE
                     NIRDAARLIDSNLFRPPYGRISRFQSALLRHEPFNYKIIMWEVLSADFDRGLSAERCL
                     RNVTRHARPGSIVVFHDSEKAWDRLRVALPGVLEHFSRSGYRFEGIPVA"
     gene            4207..4974
                     /locus_tag="VKQ52_16135"
     CDS             4207..4974
                     /locus_tag="VKQ52_16135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HLZ88786.1"
                     /translation="MQLIDTHCHLYNKEFTRDSEEVIQRAEKEGVTKFFLPAIDRHCQ
                     EALLALEARYPGKCIGMTGLHPCNVKADWETELAFVEGELARRDWVAVGEIGLDFYWD
                     RTFEKEQYIAFHRQIEWALLYQRPIVIHSRNSMKESIGVVREHQKGELRGIFHCFSGD
                     AAAAREIVELGFFLGIGGVLTYKNSGLADAIREIPLEWLVLETDAPYLAPVPFRGKRN
                     ESSYLRYVAEKLAEVKGVTVEEVAHMTTENAQKIFGN"
     gene            5050..5136
                     /locus_tag="VKQ52_16140"
     tRNA            5050..5136
                     /locus_tag="VKQ52_16140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5084..5086,aa:Ser,seq:gga)
     gene            5477..9598
                     /locus_tag="VKQ52_16145"
     CDS             5477..9598
                     /locus_tag="VKQ52_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ88787.1"
                     /translation="MNPYRLLLLLHAISLTAFGQIEGLKLKHIGTDAGLSQTNVTCIL
                     QDSRGFMWFGTRDGLNKYDGYNFTIYKNVENDKHSVSNNFITTLLEDRNGDLWVGTWG
                     GGVDRYDRNKKQFTLVDSAFANSFVNHLLQDSQGNIWICTDGEGLYKIDPATGKHKCF
                     YADTRNPSGLGDLDIYTIFEDSRHDYWVGTANGGLYQFDGKTDKFTHFAHSNTDTSSL
                     ASNSVKSIYEDAGHRLWIGTLGGGLDFLIDRKGAFRHFFSGKRAGKLRENMIQALAAD
                     SAGNLWVGTDNGGLVIIGPSGTSYQVYYQDDIDNSSLSNNSVDCAYRDRQGNMWLGTY
                     GAGANLFAKDNDNFLYYRHSSQPGSLSNNNVLDIFEDSRNDLWVGTDGGGLDLLDRRT
                     GQFRHFTSDPAKPGGISGNYVLALKEDRHGNIWAGTWGGGITVIDRTKTHFTQYRNST
                     GDPASLATNNVYDLARDKEGRIWIGTYGQGLDRFDETTHHFIHYRHDPANNNSIASDR
                     VHALLADSKGILWVGTFDGGLDRFDSKTSRFTHYRHEMRTGSLSSNSINCVYEDSTGD
                     IWVGTAEGLNRLDQTTGRITNYSVRDGLPGSVIYSVLEDGKGNYWISTNKGLAKFDPG
                     AKKVKTFTADDGLQSNDFKAHSAFKSRTGRMYFGGVNGFNAFYPDSIKENTVDPPLVI
                     TGFQIFNKEVPVSTDDKHPSPLTADITETKELKLDHNNSVITFEFASLNYTEPSGRRY
                     RYKLEGFDKDWTYTSTKRAVTYTNLDPGNYVFTVDALKGDGSWLPTPTTLRLTIIPPF
                     WMTIWFRVLAILLVVALTLTIHWLRLRNIRVHRRQLQWQVQERTAQLTLSITEERKAR
                     EEAELASKAKSEFLANMSHELRTPLNAIIGFTDLVLSTPLQQVQRDYIEHVSHAGFNL
                     LGLINAVLDYSKIEAGKLLLDDTEFNLAHLVEETVSMLAIKGFEKGLEVICEIDPHLP
                     GLFRGDSLRIQQILINLIGNAIKFTEKGEVIVQVKKEESVCIHVKDTGIGIPADKLDT
                     IFESFTQGDSSTTRKYGGTGLGLTIAKTLAEMMHGNLTVKSRLGEGTTFSLCMNLHVV
                     SEVPVTQPIRRPALRNILIVDDNASNCRLLQDIFRQMDVDCMTATSGQEALRILSAHR
                     RQSRSFDLIITDYQMPGMDGIALVREIKSMLAGRTQPFILMLSSIERNIYREEAKDIG
                     IDMFLTKPVLVEQLNQILAGIFEERNRPRPESNGHDITPVSQRQHTIMVVEDDVINML
                     LISEVLDKMGFSVLDAVNGKEALRRLETHTPCLIFMDINMPEMDGFEATRRIRELPAP
                     QCDIPIVALTADAMKEDKERCLAAGMNNFLAKPFRLEEIRTLLESYVASPVIR"
     gene            9713..10318
                     /locus_tag="VKQ52_16150"
     CDS             9713..10318
                     /locus_tag="VKQ52_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HLZ88788.1"
                     /translation="MKVSRVFILISLITLSKQSFSQGFVKGIASRLEFGLKGGANYTG
                     FTNASFPTDPLIGFHAGATVAFKITSNFLIQEEFLFSTQGAKSKGDFFTSQTNQNINL
                     YYMAVPILLKYRTNSGFYIEAGAQTGYKLKEDVAGSGTGDLFKKLDLAAAGGIGYQTK
                     IGLGIGARYVYGLSKVGDFKASDITNDFKNNAVQVSVFYVF"
     gene            10308..10553
                     /locus_tag="VKQ52_16155"
     CDS             10308..10553
                     /locus_tag="VKQ52_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88789.1"
                     /translation="MSFEIVTILGGLFPRRPHTASGRDSSIKTEQGFPEYGGLPDMDE
                     GATLLEEPELSLGTILPELHLAPGVEGPIVPPSSIPR"
     gene            complement(10564..11091)
                     /locus_tag="VKQ52_16160"
     CDS             complement(10564..11091)
                     /locus_tag="VKQ52_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88790.1"
                     /translation="MKKLLVAAISLALLALACKKSGSSSSSYYFTASMNDTAKTFNVN
                     AMAMMDTLSGIVSLSLIGNVSSAANFEGMNLTINNSPSNKPIVAGTYTENSTDFAVAG
                     VYNPGSTTIVYGAGEFPSPPNPLTIVITSIDKSSVKGTFSGDFYYTNTAVVGPVGPGK
                     KTFTKGQFYLKIQKF"
     gene            11253..>12453
                     /locus_tag="VKQ52_16165"
     CDS             11253..>12453
                     /locus_tag="VKQ52_16165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HLZ88791.1"
                     /translation="MQTSNKKAAIGFIFVTLLIDVMGWGLIIPVMGELISKLKGIPAN
                     EASPYGSYLLTAFACTQFLCAPIVGNLSDKFGRRPVLLFSLLGFGVDYIILAVAPAYG
                     WLFIGRVIAGVTGASFTTATAYIADVSTDATERAKNFGMIGAAFGLGFVLGPALGAFL
                     ATWGIRAPFYGAAVLCLLNCLYGYFVLPESLSKENRRPFDWKRANPLGSMKFLVSHPE
                     IGGLAFSFFLIYLAAQAVQGNWNFFTMYRFGWTDKMIGISLTVVGVLVGAVQAGLTRV
                     INPKIGNEKSIYLGLSLYTVGLVLFAFATQSWMMFAFLVPYCLGGICGPALQSVISGH
                     VAPNQQGELQGALTSLMSLTTIIGPLIMSGTFAYFTSRKAPFYFPGMHFLLGALFMLL
                     SVLIAVPV"
BASE COUNT         2853 a         3390 c         3495 g         2715 t
ORIGIN      
        1 gcgccgctga ggatgctgat gccgatggca tcgacgtctt cctgcagggc ggcattgacg
       61 accatctccg gcgtctggcg taagccggta tagatgacct ccatcccggc atcgcgaagc
      121 gcggtggcga taacacgggc gccacggtca tggccgtcga gtccaacctt ccccaccagt
      181 acccggatcg ggcgatcatg tgcgttctgc atgcaattcg tttagatgta aaaatatgaa
      241 tttacccttt gagcagggcc agggtatgac ggatacgccg gatcgtttct tccttaccca
      301 aaaggacggc gatgtcaaac acatgcggtc cgaattttcc tcccaccagc ataacgcgca
      361 ggggcatgag caactcaccg ggcttgatag ccgccgcggc ggccatctcc ttgaactggg
      421 tttcgagggc atgtgcgtcg gcatcggttg ccagttcgaa ctgccggatg tattcggcaa
      481 agaaaagggc cttggcgtcc gtccacttcg gcttcacggc ctccaggtcg atggtggcgg
      541 gtggctggaa gaagaacgcc gcctgcggga cgaattcggg caggagggca caacggtcct
      601 tcaccaggtc gagcacgcgc tcgaagcgat tgggcggagg cggagccggt tcgagaccgg
      661 attcgagtgc ggccaggtcg atgagcggtg ttccgctgat atcggtgagc gtgtcgggga
      721 tgagcgggat gacggggggc aggtagtcgg gttccgggtc ggcgggacgc aggagatcgg
      781 ccggggggat ggtgatcccc gcggcggcga agaggggggc cacctgtgga gcataggcgg
      841 aggcgggcag ttgtttgatc cattcgtggt tgaaccattt ggccttctcg aaatcgaatt
      901 tggcgccacc cttatggacg cggaccaggg agaatttctc cacgagctcg gacagggtaa
      961 agatctcctg ccccgagccg tcgttccagc caagcagggc cagcagattc acaaaggcct
     1021 gcgggagaaa gcccgcttcg cggaagccgg tagcgggttc attggtgcgg gggtcggtcc
     1081 agttcatggc aaataccggg aagcccaggc gatcgccatc gcgtttactc agttttccat
     1141 tgccgtcggg cttcaggatc aggggcagat gggcccactg gggcatcttg tcctcgccaa
     1201 agagataacg ccagagcagg atatgcaccg gtgcactggg cagccattct tcgccgcgga
     1261 aagcatgcgt gatcgccatc agatgatcat cgacgaccac ggcaaaatga taggtaggca
     1321 tgccgtcggc cttgagcaat accttgtcat cgacacccgt ggtggaaaaa tggacctcgc
     1381 cacggataag atcggtaaat cgcacctcct ggtcgggttc gagccggatg cggatgacat
     1441 agggagtgcc ttcggccagg agacgtttgg tctccgccgg ttcgagcgac aggggattgc
     1501 gcattttttt gcgcagcgtg tggttgtatt ggggggaggg attgtccgcc gtcttgtatt
     1561 tctcccgcat cgcctcgagc tctcctggag tatcgaaggc gtaataggca tggcccgcat
     1621 cgacgagttg ctgcgcatat tttccgtaga tgccaagggc cttgcgctcg ctctggcgat
     1681 aggggccgta ggcgccaccc ttgtgagggc cctcgtcggg ttcgaggcca caccagcgga
     1741 ggctgtcgat aatatattct tcggccccgg gtacataccg ggcctgatcc gtatcttcaa
     1801 tgcgcaggat gaagtcgccg ccatggcgcc gggcgaaaag gtagttgtag agaacggtcc
     1861 ggacgccgcc taagtgtaac cccccggtgg ggctgggtgc aaaacgcact ctgacttttt
     1921 tgctcatagg gggcaaaggt aacggatatt cacggagtga atatcgcgga acgaagtgga
     1981 gcagatattg gccccccaga aggccgtcga cgacggccgg agccgggcgg agaggttggt
     2041 aaaccaggta gaggctggcc caataatacc ccggatttga cgttaatgag tatcgggtat
     2101 gtccaaaata ctgcaacttt atccacgaat ggaaaacgcc gccacacctt aaaataattc
     2161 cgatcgtgaa aatttcgctc accttttgcc tgcttttttc ccttttgtct gcctctgccc
     2221 agcttacgta tgagaccctg ttcgtggatt atgatagcgc ctggcaatac aagaatttaa
     2281 agatcattcc catccgccgc aaggctggag ccgggatggc gatgcagcac ggcgatatcg
     2341 tgccgcttgg ccaggccctc agcacggggc aggtgacggt caccgaacgc gggactactt
     2401 cgttcgagaa tgtccactgg ctccgattca acacccatgg caagaaaccg gtgtacatcg
     2461 ccggagggga gatcatcgcc ggaggacggc aggacaggat gatcgttcgc gatacgatcc
     2521 tgcaaccgat ggataaggac cagtacgtgc ccgtgatgtg cgtggaagag ggacgatgga
     2581 gcgacaagga aaagaagttc ggatatggga attttgccaa cccgcattta cgaaaggtgc
     2641 tggattcgac gggtaaccag gtgtcgatat ggcttgaggt ggaccggcag ttgcaggccg
     2701 gcgcgttcaa gaacaaaacg ttctcctatc tttcgcggta tgaggataag aaatacctgg
     2761 cggcacagga tgactatttc cggttcttca tgaacaaatt tgcaaagacg gacagcaatg
     2821 tggtcggttt cgttgccata acgggagata agatcatcgg cacggatatc tttgatggta
     2881 ccgatctgtt ctatccgcaa ctggaccccc tgttgcacgg atatatcgac gatgtgatcg
     2941 cgtcgggttt gccggtgaac ataaaagagc cgcccgtgaa acgatatatg gatcgcctgt
     3001 tgaaggatga gaaaagccag gaagagtttg taaaggacaa gggaaaggtc ttccggcaga
     3061 atggccgcgt gatccacgtg aatacctata caaaggaaac gccggtgcaa taaaatcgcc
     3121 tatcttgcct gctatgttct acctggtacg atcgccctgg tggctgaaaa gattctattc
     3181 gcggcttgtc tgggatatcc ccgcaaagga taaggttctc tacctgacat ttgatgatgg
     3241 tccgcacccg gtggccacgc cgttcgtgct ggatgaattg aagaagcatg atgcccgcgc
     3301 cacttttttt tgcatcggca agaacgtaca ggagtacccg cagatctatc gccgtgtttt
     3361 gatggagggg catcgggtgg gcaaccatac acagaatcat ctcaacggat ggaagacgga
     3421 cgacaagcgc tatctcgaga atatccggga tgccgcgcgg cttatcgact ccaatttgtt
     3481 ccgcccgccc tatggccgca tcagccgttt tcaatccgcc ttacttcggc atgagccgtt
     3541 caactataag atcattatgt gggaagtgct tagcgccgac ttcgaccggg gactgtcggc
     3601 ggagcggtgt ttgcgcaatg tgacccggca tgcgcggccc ggatcgatcg ttgtttttca
     3661 cgatagcgag aaggcctggg acaggttgcg ggtggcattg ccgggggtgc tggaacactt
     3721 cagcagatcg ggatatcgtt ttgaagggat cccggtggcg tgagcggttt tgcggctata
     3781 ctagtgaggg atgaggggat ggtgggccgg ttgacaagag ggggatgggc ggcatgccgc
     3841 gaggcgaatt tcccgtactg ggaatgatgg tgtaggaacg gtttttgcta aaaataaaat
     3901 tgggcctggg tagacaccct ggcccgtttt taatccgtac cctatgaaaa ctggtttaaa
     3961 ggtaaatctt tttttcattt gtgcaaagta atttagtcag tcggggctga cgaatcttct
     4021 ggaatagggc cttttaatac cattttccct aatttgtcta tttctttttt cgatgcgtgc
     4081 aatccgcgtc catcccgagt tttagccatt tccgggttga tcggcgaggg tgctttcgca
     4141 aaatgggaat agtccggatc atgggttttg gggatatttg tacccaccat ttggacaaaa
     4201 caggatatgc aactgataga cactcattgc catttatata acaaggagtt tacccgcgat
     4261 agcgaggagg taattcaacg agctgaaaaa gaaggagtta ctaaattttt cctcccggct
     4321 atcgaccggc attgccagga ggcgttgctg gcattggagg cccggtatcc cggaaagtgc
     4381 atcgggatga cggggctgca cccttgtaat gtgaaagcgg attgggagac ggagttggca
     4441 ttcgtggagg gggaactcgc acgccgggat tgggtggcgg tgggagagat cggactggat
     4501 ttttactggg atcgaacatt cgaaaaggag caatatattg ccttccaccg gcagatcgaa
     4561 tgggcgttgt tatatcaaag gccgatcgtg atccactcgc ggaattcgat gaaagagagt
     4621 atcggcgtgg tgcgggagca tcagaaggga gagctgcggg gcatcttcca ctgtttcagc
     4681 ggcgatgccg cggctgcgcg ggagatcgtg gagttggggt tcttcctggg catcggcgga
     4741 gtgctgacct acaagaattc cggcctggcc gatgcgatcc gggagatccc gctggagtgg
     4801 ctggtgctgg agacagacgc gccctatctc gcgcccgtac ctttccgcgg caaacgcaac
     4861 gagagcagtt acctgcgata tgtggcggag aagctcgccg aagtaaaggg ggtgacggta
     4921 gaggaggtgg cccacatgac gacggaaaat gcacaaaaaa tattcggaaa ctaggcacgg
     4981 aagccgtaca tttgcagccc tcgtaaaggg gttggccggt tggctgtagt tgataaccgg
     5041 ccgatgatcg gagagatgtc cgagtggttg aaggagcacg cctggaaagt gtgtatactc
     5101 gaaagggtat cgcgggttcg aatcccgctc tctccgcaaa cgcaagcacg gcagtgctca
     5161 gccttttggc tgggcatttt ttattccggg tagcgggcaa gcttgcttgc caaagcggac
     5221 cgggataaaa aatgacgcac gtagtgcggc actgctgtgc ttgcgtttaa gcccctcccg
     5281 caagggatca gcgtagctaa tcccactttc tccgcatttt caaacaccgt gatgccctaa
     5341 cgagtaacgt attaggctac ttcagagcat aagtgttttc tcttatatcg aaaccctccg
     5401 atgcactgtg ctaaaatatt tgcctgtccc gccacaataa gaattgtttt taatgtgttg
     5461 gtgtaggtgt aatcttatga acccttaccg tctactctta ctattgcatg ccatatccct
     5521 taccgccttc ggccagattg aaggactaaa gctgaaacac atcggcacgg acgcaggcct
     5581 ctcccagacg aacgtcacct gcatcctgca ggacagccgg ggttttatgt ggtttggaac
     5641 ccgtgacgga ctcaataaat atgatggcta caatttcacg atctataaga acgtcgagaa
     5701 cgacaagcat tccgtcagca ataactttat cacgaccctc ctcgaagacc gcaacggaga
     5761 tctctgggtg ggaacatggg gcgggggagt agatcgctac gaccgcaata aaaagcaatt
     5821 caccctcgtc gattccgcct ttgccaactc cttcgtcaat cacctgctgc aggattccca
     5881 gggcaatatt tggatctgca ccgacggcga aggcttgtat aaaatagatc cggccacggg
     5941 aaaacacaaa tgcttttatg cggacacaag aaacccctcc ggcctgggcg acctcgatat
     6001 ctacacgatc ttcgaggaca gccgtcacga ttattgggtg ggtacggcaa acggcgggct
     6061 ctaccaattc gatggaaaga cggataaatt cactcacttt gcccacagca atacggatac
     6121 ttcttccctt gcctccaatt cggtaaaaag catctacgag gacgccggcc accggctctg
     6181 gatcggcacg ctcggtggcg gcctggactt ccttatagat cgcaaaggcg ccttccggca
     6241 ttttttcagc ggcaaacgtg ccgggaagct aagggagaac atgatccagg cgctcgccgc
     6301 cgatagcgcc ggcaatctct gggtcggcac ggacaacggg gggctcgtca tcatcggccc
     6361 ctcgggcaca tcctaccagg tctattacca ggacgatatc gataacagca gcctatccaa
     6421 caactccgtc gactgtgcct acagggacag acaggggaat atgtggctgg gaacctatgg
     6481 cgcgggtgct aacctcttcg caaaagacaa cgataatttc ctgtactacc ggcattcctc
     6541 ccaacctggc agcctcagca acaataacgt gctcgatatc ttcgaggatt cccggaatga
     6601 tctctgggta ggtaccgacg gcggcggcct cgatttactc gaccgtcgca ccgggcaatt
     6661 ccgccacttc acatccgatc cggcgaagcc tggcggcatt agcgggaatt atgtgctggc
     6721 cctgaaggaa gatcgccacg gcaacatctg ggccgggaca tggggcggag gcatcaccgt
     6781 tatcgatcgc acaaaaacgc acttcaccca atacaggaat agtaccggag atccggcaag
     6841 cctcgccacc aataacgtat acgatctcgc ccgcgataag gaagggcgca tctggatagg
     6901 cacctatggt cagggtctcg acaggttcga cgaaacaaca catcacttta ttcattaccg
     6961 gcacgatcct gccaacaata acagcatcgc cagcgaccgc gtccatgccc tgctcgcgga
     7021 cagcaaaggc atcctctggg tgggcacctt cgacggcggt ctcgatcgct tcgatagcaa
     7081 aaccagccgc tttacccact accggcatga gatgcgcaca ggcagtctga gcagcaattc
     7141 catcaactgc gtctatgagg acagcacggg tgatatctgg gtcggcaccg cagaaggcct
     7201 caatcgcctc gaccagacca ccggccggat aaccaattat tccgtcaggg acggtcttcc
     7261 gggatcggtc atttacagcg tgttggaaga tggcaaaggc aattattgga tcagcacgaa
     7321 taaaggactg gcgaagttcg atccaggagc aaagaaggtg aagaccttca ccgccgacga
     7381 cggtctccag tccaacgatt tcaaggccca ttccgccttt aagagccgga cgggaagaat
     7441 gtatttcggc ggcgtcaacg gcttcaacgc cttctatccg gacagtataa aagagaatac
     7501 ggttgatcca cccctggtga tcaccggctt tcagatcttc aacaaggagg tccccgtttc
     7561 caccgacgac aaacaccctt ccccgttgac cgcggatatc accgagacaa aagagctgaa
     7621 gctcgatcac aataattcgg tcatcacctt cgagttcgcc tcgctcaact atacggaacc
     7681 aagcggccgg cgatatcgct ataagctcga aggattcgac aaggactgga cgtataccag
     7741 taccaaacgc gccgtcacct ataccaacct cgaccccggc aattacgttt tcacggtaga
     7801 tgcgctgaaa ggggacggca gctggctgcc tacgcccacc actctccggc tcaccatcat
     7861 cccgcctttt tggatgacca tctggttcag ggtgctggcg atcctcctcg tcgtcgccct
     7921 gacgcttacc atccactggc tcaggctccg aaacatccgg gtacaccgca gacagttgca
     7981 atggcaggtg caggaacgta cggcgcaact gacgctctcc atcacggaag aacgcaaggc
     8041 ccgtgaagaa gccgagctgg ccagcaaggc gaaaagcgaa ttcctggcga acatgagcca
     8101 cgagctgcgg acacccctca acgccatcat aggatttacc gatctcgtcc tgtcgactcc
     8161 tctccagcag gtgcagcggg actacatcga acacgtaagc cacgccggct tcaacctcct
     8221 cggcctgatc aacgccgtcc tcgattattc caagatagag gcggggaagc tcctcctcga
     8281 tgacactgag ttcaaccttg cccacctggt ggaagagacg gtatccatgc tcgcaataaa
     8341 aggatttgaa aaaggcctcg aggtcatttg cgaaatagat ccccacctgc ccgggttgtt
     8401 ccgcggcgac agcctccgca tccagcagat cttgatcaac ctcatcggta acgcgatcaa
     8461 gttcaccgaa aaaggcgagg tcattgttca ggtgaaaaaa gaagagtctg tctgcatcca
     8521 cgtaaaagat accggcatcg gcatcccggc cgataagttg gatactattt tcgaaagctt
     8581 cacccagggc gattcctcca ccacgcgcaa atacggcggc acggggctcg gcctcaccat
     8641 cgcaaagacc ctggccgaaa tgatgcacgg caacctaacg gtaaagagca ggctgggcga
     8701 ggggacgaca tttagcctct gcatgaacct gcacgttgtc agcgaggtgc cggtcacgca
     8761 acccattcgt cgccctgcct tacgcaacat cctcatcgtc gatgacaacg cttccaactg
     8821 ccgcctcctc caggatatct tccgccagat ggacgtggac tgtatgacgg ccaccagcgg
     8881 ccaggaagcc ctccgcatcc tctccgccca tcgccgccag agccggtcct tcgatctcat
     8941 catcaccgat tatcagatgc ccggtatgga tggcatcgcc ctggtcaggg aaataaagtc
     9001 gatgctcgcc ggtcgcaccc aacccttcat tttgatgctc tcatcgatag aaaggaacat
     9061 ctatcgcgaa gaagcaaagg atatcgggat cgatatgttc ctgacaaaac ccgtgcttgt
     9121 cgaacagctc aaccagatcc tcgccggtat cttcgaagaa aggaaccggc cgcgtccgga
     9181 aagcaacggc catgacatta ctcccgttag ccaacggcaa cacactatca tggtggtgga
     9241 agatgacgtg atcaacatgt tgctgatctc cgaggtcctc gacaaaatgg gattctcggt
     9301 cctcgatgca gtcaatggaa aggaagccct ccgacgcctg gagacccata ccccctgcct
     9361 catcttcatg gacatcaaca tgcccgaaat ggacggcttc gaagccaccc gccggatacg
     9421 ggaactcccc gcgccccaat gcgatattcc tatcgtcgcc ctcacggccg acgccatgaa
     9481 agaagataag gaacgctgcc tcgccgccgg catgaataac ttcctggcaa agcccttccg
     9541 cctcgaggag ataagaaccc tcctcgaatc ctatgtcgcc tcgcctgtca tccggtaatt
     9601 acccttcctt gccctattcg cttcccccct ccttttgtac gcttcaggcc aatacgcctt
     9661 ccggcatcgg gccgactgct gcaattttgt tctcaaattc aaatacagca ttatgaaagt
     9721 gtcaagagta tttatcctta tcagccttat caccctatcg aaacagtcgt ttagccaggg
     9781 ctttgtcaaa ggtatcgcta gccggctcga gttcggattg aagggcggcg ccaattacac
     9841 cggtttcacc aacgccagtt tccccaccga cccgctgatc ggtttccatg cgggcgcaac
     9901 ggtggctttt aagatcacca gcaacttcct gatccaggaa gagttcttat tctccacgca
     9961 gggcgccaaa agcaagggtg actttttcac cagccagacc aaccagaata tcaatctcta
    10021 ttatatggcc gtccccatcc tgttaaaata ccgcaccaac tcagggtttt atatcgaggc
    10081 aggtgcacaa accggctata agctcaagga agatgtcgcg ggatcgggca ccggcgatct
    10141 tttcaagaag cttgaccttg ccgccgcggg gggcatcggc taccaaacga agataggtct
    10201 gggcatcggc gcacgatatg tatacggtct gtccaaggtc ggcgatttca aggcctccga
    10261 tatcaccaac gactttaaaa ataacgctgt acaggtaagt gtgttctatg tcttttgaga
    10321 ttgtaacgat cctcggcggg ttgtttccca gaaggccgca caccgcatca ggcagggata
    10381 gcagcataaa aacggaacag ggtttcccgg agtatggtgg gctccccgat atggatgaag
    10441 gagccactct cctcgaagag ccggaactca gcctggggac aatactgcct gaactccatc
    10501 tggcaccggg ggtagagggc ccgatcgtgc cgccttcttc tatcccgcgc tgaccaggcc
    10561 ccgtcagaat ttttggatct tcagatagaa ctgccccttg gtgaacgtct tcttcccggg
    10621 accgactgga ccgacaacag cggtgttcgt gtagtagaaa tcaccgctga aagtgccttt
    10681 cacactgctc ttgtcgatcg acgtgatgac gatcgtcagc ggattgggcg ggctgggaaa
    10741 ctcacctgca ccatatacga tggtcgtgct gcccggattg tagacgccgg ccacggcaaa
    10801 gtcggtgctg ttctcggtgt aagtacccgc cacgatgggt ttgttgctgg gagaattgtt
    10861 gatcgtgagg ttcatgcctt cgaaattcgc tgccgagctc acattgccga tgagggaaag
    10921 cgaaacgatg ccggacaagg tgtccatcat cgccatcgcg tttacgttga aggtcttcgc
    10981 cgtgtcgttc atggaggccg tgaaataata ggagctggaa gaagatcctg attttttaca
    11041 ggcaagggcg agaagggcga gcgagatcgc agcgacaagt aattttttca tggactatca
    11101 gtttaggata gtccaaaata atcaaaagct gcgatatttc caagggatga cgggagcgac
    11161 tccggtgagt gtcgggatca cctcggtaag ttgacgcttt cacacccccg gagtttccgg
    11221 tttttggagt atgtttgttg catactaatc atatgcaaac atcaaacaag aaggccgcca
    11281 tcggttttat ctttgtcacg ctgctcatcg acgtgatggg atggggattg atcattccgg
    11341 taatgggaga gctcatctcc aaattgaagg ggatacccgc caacgaggcc agcccatatg
    11401 gctcgtatct gctcaccgca tttgcctgta cccaattcct ttgtgcaccg atcgtcggca
    11461 accttagcga caaatttggg cgccggcccg tcctgctctt ttccctcctg ggttttggcg
    11521 tcgactatat catcctcgcc gtggcgccgg cctatggctg gttgttcatc gggcgggtga
    11581 tcgccggcgt tacaggcgcc agctttacca ccgccaccgc atatatcgcc gatgtcagca
    11641 ccgatgcgac agagcgggca aagaatttcg ggatgatcgg cgccgccttc ggcctgggct
    11701 ttgtcctcgg gccggcactg ggggccttcc tggccacctg gggcatacgc gcgccgttct
    11761 atggagccgc ggtgctctgc ctgttgaact gcctctacgg ctatttcgtc ttacccgaat
    11821 cgctaagcaa ggaaaaccgc cgccccttcg actggaagcg tgcgaaccca ctcggctcaa
    11881 tgaagttcct ggtctcccat ccggagatcg gtggattggc cttcagcttt ttcctgatct
    11941 atctcgccgc ccaggcagta cagggtaact ggaatttctt taccatgtat aggtttgggt
    12001 ggaccgataa aatgatcggc atctcactga cggtagtcgg tgtgctcgtc ggcgccgtac
    12061 aggccgggct cacccgggtg atcaatccaa agatcggcaa cgagaagagt atctaccttg
    12121 ggttgtccct gtatacggtg gggttggtcc tcttcgcctt cgcgacccag agctggatga
    12181 tgttcgcctt cctggtgcca tattgccttg gaggcatctg cggaccggcc ctgcagtccg
    12241 ttatctcggg acacgtggcg cccaaccagc agggcgagct acagggggca ttgacaagcc
    12301 tgatgagcct gacgactata atcggtccat tgatcatgag cggcaccttc gcctacttta
    12361 ccagcaggaa agcaccgttc tattttcccg ggatgcattt cctgcttggc gccctcttca
    12421 tgctgcttag cgtactcatt gccgtgcccg tgc
//
LOCUS       DATGFG010000857         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_842300, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000857 DATGFG010000000
VERSION     DATGFG010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842300"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VKQ52_16170"
     CDS             <1..1277
                     /locus_tag="VKQ52_16170"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ88792.1"
                     /translation="LDTSFLADPVVDGLLLAVAAGTSFLAGFYPALVLSGFDPIMAFR
                     NSTSPRGSGSHLLRRVLVVLQFTISQALIICVLIVMGQVDYFRSAPMGFDKDAILITH
                     MPDNKKMDYLRQQLLSRPGVRNVSFSFASPNDVNSDWNTDITYNGKKMHDFGVNLKWA
                     DSVYPHLYHMQLLAGRLITRGDDVIVNEAFLRKLGIRRPQDALGAKLGVNGINDSDGV
                     ITGVVRDFNIASLHDSVRPAMIQEWKEVYYTVNIKLSAGMISQALPAIEQLWKSTFPR
                     DVYEYQFLDESIARYYEQEARLSVMYRLFAVLAVCISCLGLYSLASFMAVTRAKEVGI
                     RKTLGASVIDIVHLFSREFTSLVFLAFLIAAPVAGYLMHRWLLNYAFHFRPGPLLFIE
                     AVGISVGIACLSVGYRALRTALANPVRSLRTE"
     gene            1479..1772
                     /locus_tag="VKQ52_16175"
     CDS             1479..1772
                     /locus_tag="VKQ52_16175"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLZ88793.1"
                     /translation="MNIYVSGLSFSIADEELKQLFAEHGKVSSAKVIMDKYSNRSKGF
                     GFVEMPDKDEAEKAIKQLNGFHFEGRSIVVNEAKPREERSGSGDRRSVSGNRW"
     gene            1875..2129
                     /locus_tag="VKQ52_16180"
     CDS             1875..2129
                     /locus_tag="VKQ52_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88794.1"
                     /translation="MTSDEIEQFLTRQKINESQQVKIIFKKRDAMNGTFIKGTDYAEL
                     KRKNFWRIVTAGRHEEWVTSKSNSSARLFNGEEFSRLTLK"
BASE COUNT          594 a          641 c          605 g          654 t
ORIGIN      
        1 atcttgacac ctcctttctc gcagatcccg tcgtggacgg cttgctgctc gccgtggctg
       61 ccggaaccag tttcctggca gggttctatc ccgccctggt attgtccggg ttcgacccca
      121 tcatggcctt caggaacagt acgtcgccca ggggcagcgg atcgcacctc ctgcgtcggg
      181 tcctcgtcgt gttacagttt acgatctccc aggcgctgat catttgcgta ctcattgtca
      241 tgggccaggt ggactatttt agatcggcgc cgatgggctt cgacaaggac gccatcctca
      301 ttacacatat gccggacaat aaaaagatgg attatctgcg tcagcaactc ctctcccggc
      361 cgggcgtcag gaacgtgagc tttagttttg ccagccccaa cgatgtgaac agtgactgga
      421 acaccgatat aacctacaac ggtaagaaga tgcacgattt cggggtcaac ctcaagtggg
      481 ccgattccgt ctatcctcat ctttaccaca tgcaattgct ggcgggccgc cttatcacca
      541 ggggcgatga tgtcatcgtg aacgaggcct ttttgcggaa actgggtatt cgccggccgc
      601 aggacgcgct gggcgcgaag ttgggagtca acggtataaa cgattcggat ggcgtcatca
      661 cgggggtggt cagggacttc aatatcgcct cactccacga ttcggtcagg ccggcgatga
      721 tacaggaatg gaaggaggta tactacacgg ttaatataaa attgtcggcc ggaatgatca
      781 gccaggcgct ccccgctatt gaacaactgt ggaaatcaac cttccccagg gacgtctatg
      841 aatatcaatt cctggatgaa agcattgccc gatactatga acaggaggcc cggttgtccg
      901 taatgtaccg gctttttgcg gtactggctg tatgtatatc ctgcctgggc ctctatagtt
      961 tggcctcttt catggctgtt acccgtgcta aagaagtagg catccgcaag acactgggcg
     1021 cctcggtgat cgatatcgtt catctgttct cccgggaatt cacttcactc gtcttcctgg
     1081 ccttcctcat cgccgcgccc gttgcgggct atctcatgca tcgatggctg ctgaattacg
     1141 ctttccattt tcggccggga ccactgctgt tcatcgaagc cgtcggcatc tccgtaggga
     1201 tcgcctgttt atccgtcggc tatcgggcac tcaggaccgc actcgccaat cccgtcagga
     1261 gccttcgaac ggagtaaaac ctagcctccc ggcacccact cacggggcgg tttcctaaat
     1321 aaatttgcat acatgccata attagcgtac ttttagggtg ttaatttgag ttttacagca
     1381 ctcagtaagt gaaatttccc gcggcacagt cgacagtcaa atcttatttt actccttgct
     1441 ttttatctca agcattattc gttttttaat ttcaaatcat gaacatttat gtgtcgggct
     1501 taagcttctc catcgcagat gaagagttaa agcaattgtt tgccgaacac ggcaaggttt
     1561 cttcagcaaa ggtaattatg gacaaatact ctaaccgcag caagggtttt gggtttgttg
     1621 aaatgcctga caaggatgaa gccgaaaaag ccatcaaaca acttaacggc tttcatttcg
     1681 aaggaaggtc gatcgttgtg aacgaggcaa agccccgcga agaaaggtct gggtctggcg
     1741 acagacggtc agtcagcggc aacaggtggt aataccgtta atgcaatatt ggagagagcc
     1801 ggctaccggc tctctttttt catttggaat atcaacgttc tttgttgtaa agtataacat
     1861 aaaacttagc tactatgacc agtgacgaga tcgaacaatt cctgaccagg caaaagataa
     1921 atgaaagcca acaggttaag atcatcttca aaaaaaggga tgcaatgaac ggcaccttca
     1981 taaaggggac cgattatgca gaactcaaac ggaagaattt ttggcgcatc gttacagcag
     2041 gacgccatga agaatgggta acctcaaaaa gtaatagcag cgcccgcctg ttcaacgggg
     2101 aggaattttc acgtctgacg ttgaaataat tctcaccgcg ccatctaatt attttttcac
     2161 aatgctgcgg gtcgtccttt ctactatggc tatgcgatcc aattacttgt agggttgtat
     2221 aagtgttttc cttaggcgcg aatccggacc accctgggga atccacttgc atttttggat
     2281 tttgcgtcct actttcacta ccgctaatcg gtatcctatt agaagccgct aaatcctaac
     2341 cctcatgaac catgaaaacc ctttcctggt accggtgcac ctgcgccgtt gccgacctgc
     2401 gaccaagtcc gaaaagtttt cccgccggta tttcgctttt ccttttatta tcttattgtt
     2461 gttgagcagt gcgccttccc tggcccagac catg
//
LOCUS       DATGFG010000858         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_160643, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000858 DATGFG010000000
VERSION     DATGFG010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160643"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VKQ52_16185"
     CDS             complement(<1..216)
                     /locus_tag="VKQ52_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88795.1"
                     /translation="MKQNTKWLMTALAVAGGLIIANSSQAQYIFGNGAAAGIAMGGGS
                     FPTPNTTTATGLSLTGSGYNWGEWDIPV"
     gene            616..1647
                     /locus_tag="VKQ52_16190"
     CDS             616..1647
                     /locus_tag="VKQ52_16190"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLZ88796.1"
                     /translation="MVRIEKDHAMKYKMDIETNRFERDELSINPFQLPRGSEREKSLS
                     GAFTLIELLVVIAIIAILAAMLLPALAAAKSRALAVACLSNTKQIALGFTMYAGDNHD
                     YFPANEQLNGGFWWLPGGYFNKYLLPCGGEWMARSPVYGSVPNTPAPMLVSYLPNNKA
                     WVCPARKRGLTYPSAPGVWDPSITGLLSYGFNYCGVFSAVNPADGNMINAKPFRASSA
                     LKPSLLVAVTDSSGSRDPSGKGAAWLDSYWAGSSGPSGDPGGTSEYSARLQTAYAKHN
                     NRVNVVYVDGHAAPSLPSALTWGQFYGVFTAGVPLKTSPSTPNPSVISSDPISTPALD
                     VKQWSTTPE"
     gene            1991..>2988
                     /locus_tag="VKQ52_16195"
     CDS             1991..>2988
                     /locus_tag="VKQ52_16195"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HLZ88797.1"
                     /translation="MEASGGQAVELTGTGQYVELTSQAAANAMVVRYCVPDTANGTGT
                     NSTISLYVNGTFVKKLPVTSIYSWRYGAYPFSNTPSQGKPRNFFDEVRLNGLTINAGD
                     TVRIEKDADDTAAYDVIDLVDLENVAPPLTVPPNSLSVISYGADTNGVIDSTTALQNC
                     VNAAASQSKTVWIPAGTYAISATINLPSNTSIQGAGMWYSELMGIASLYNTTPSRRIT
                     LNGSGSSIHLADFAILGFLNYRNDSEPNDGLGGSYGTGSTVSRIWVEHTKTGAWIVNS
                     SGLVIDSCRFRNTIADGCNLAVGMQNTTVTNCTTRGTGDDCFAIWPATYTSQSYKPG"
BASE COUNT          694 a          793 c          752 g          749 t
ORIGIN      
        1 caccgggatg tcccactcac cccaattgta accggaaccg gtaagtgaaa gtccggtcgc
       61 cgtagtcgtg tttggtgtcg gaaaacttcc accgcccatg gcgatgccgg ccgcagcgcc
      121 gttcccgaag atatattggg cctgtgacga gtttgcaatt attaaaccac ctgccacggc
      181 caaggcggtc atcagccatt ttgtgttctg tttcataacg ttttgtctct tttgattttg
      241 ctaattttta atgttccgct tctaaaatca aatccgacca acaatttatt ccgatgcttg
      301 aaattatttt caagacaccg gagaagttga gtctagaaca tgattttcac tgctagtgac
      361 actagcagaa tgaatacctc ttgccagtcg catgaactgg cgacaagcga tgcaataacc
      421 gaatataacg attagccata aatcatttat ggaagagcaa cctgccggaa tatccgggtc
      481 tggggaatca attctgttcg gcgagtcatg taattgtccc cgttttatgc gactataata
      541 gctgaaggga ggtgttaagc tgcagtattg ctaggttcga ttttgtggat attggaatca
      601 tgccatgccg ggcggttggt tcgcatagaa aaagatcatg ctatgaaata taaaatggac
      661 atcgagacga acaggtttga acgagacgag ttgtcaataa atccttttca gcttccccgt
      721 ggcagcgaaa gagaaaagtc tttgagcggg gcttttacgt tgattgagtt gctcgtcgtc
      781 attgccatta tcgccattct ggcggccatg ttgcttccgg ccctggccgc tgcgaagtcg
      841 cgcgcactgg cggtagcttg tttgagtaat accaagcaga ttgcacttgg ctttaccatg
      901 tatgcgggcg ataaccacga ttattttcca gcaaacgagc aactcaacgg tggattttgg
      961 tggttaccgg gagggtactt caataaatat ctcctgccct gcggaggaga gtggatggct
     1021 agatctcctg tttacggttc ggtacccaat actccggcgc caatgctggt ttcttatcta
     1081 ccaaataaca aggcttgggt atgccccgcg cgtaaacgcg gtttgactta cccttctgca
     1141 cccggagttt gggatccgag cattaccggt ctgctttctt atggattcaa ttactgtggt
     1201 gttttttccg ctgtgaaccc tgccgacggc aacatgatca atgctaaacc gttcagagct
     1261 tcttcagctt tgaagccgtc actattggta gctgtcactg atagcagcgg ttcccgtgac
     1321 cccagcggca aaggtgccgc ctggttggat tcgtattggg cgggatcttc cggtccttca
     1381 ggagatcctg gcggaacaag cgagtatagt gcccgccttc agaccgcata tgccaaacac
     1441 aataatcgtg tgaatgtcgt ctatgtggat ggtcatgctg cgccctcgct gccaagtgct
     1501 cttacctggg ggcagttcta cggagttttt actgctggcg tcccactcaa aacctctcct
     1561 tcaaccccga atcccagcgt gatatcgtcc gatcccatca gcactccggc tttggatgtg
     1621 aagcaatgga gtaccacgcc ggaataagac tttgccatcg gacaatcagg catggtaaat
     1681 tccttttaaa agatctatat tgaccaagga tgccaaccaa aacgattgcg aattaattgt
     1741 tgcctttaca cgctaaaggc tcgccgtaca aattgacgtc gcctccctgt ttcatatgtc
     1801 atgcccgccg ctgcaactcg caatcatcct gttcctcggt gcctcgctat gctcactcca
     1861 ggcatctccc ggtgcgaatg tcccctggac cacctacgaa gccgagaaca tgacgatcaa
     1921 tggcggaacc gtgatcggcc cgccgacgca ggttgctgac atcaatgctc ccgtgtcgaa
     1981 tacaattcaa atggaagcgt cgggcgggca ggccgtcgag ctgaccggca cgggtcaata
     2041 cgtcgaattg acgtcgcagg ccgcggccaa tgccatggtg gtgcgttatt gcgtgccgga
     2101 tacggccaac ggaaccggga ccaattcgac catcagcctt tacgtcaacg ggacctttgt
     2161 caaaaaactt ccggtgacat ccatctattc ctggcgatat ggcgcgtacc cgttctccaa
     2221 tactcccagc cagggtaaac cgaggaactt cttcgatgaa gtccgtctga acgggttgac
     2281 catcaatgca ggtgacactg tacgcattga gaaggatgcc gacgacaccg cggcgtatga
     2341 cgtcattgat cttgttgatc ttgagaatgt cgctcccccg ctaactgtgc ccccgaattc
     2401 actgtccgtc atcagttatg gggcggacac gaatggggtc attgattcga ccactgcatt
     2461 gcagaattgc gtcaatgccg ccgcatccca aagcaagaca gtctggatac cagccggaac
     2521 ctacgctatc agcgcaacca ttaatcttcc ttccaatacc tccatccaag gcgccggcat
     2581 gtggtacagc gagctgatgg gcattgcatc cctgtacaat accactccct ccagacggat
     2641 caccttgaat ggctcgggaa gtagcattca cctggctgat tttgccatat tgggcttcct
     2701 gaactatcgg aatgacagcg agcccaacga tggtctgggc ggttcctatg gcaccgggtc
     2761 caccgtttcg cggatctggg tggagcacac caagactggc gcgtggatcg tcaattcaag
     2821 cggcctggtg attgacagtt gccggttccg taacaccatc gccgacggct gcaatcttgc
     2881 ggtgggaatg caaaatacta ccgttactaa ttgcaccacc cggggtacgg gcgatgattg
     2941 cttcgccatc tggccggcga cttatacttc acagtcatac aaaccggg
//
LOCUS       DATGFG010000859         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_43838, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000859 DATGFG010000000
VERSION     DATGFG010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43838"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..>2082
                     /locus_tag="VKQ52_16200"
     CDS             90..>2082
                     /locus_tag="VKQ52_16200"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HLZ88798.1"
                     /translation="MALENEINQRKFRTEYQKARINIIYTYNWLSEKINSIFEEWDIT
                     PRQFNILRILRGEGKPLSTLQIRQRMLDKMSDTSRIVDRLLKKGLVRKTPNSGDRRLV
                     DVVITSKGKKLLEKIDPFEDHADKFMTSLSEEETKTLNGLLDKLRNLSCVILLLVLTA
                     AGVSAPATAFSQPAASTSSLPAPVMPKYEFRAAWVASVENIDWPSKKGLPVDSQKVEF
                     TRLLDMHKRNGLNAVVVQIRPAADAFFPSSYEPWSEWLTGVQGKAPDPWYDPLSFMIE
                     ETHRRGMEFHAWCNPYRAVKTIGRSSVAADHITRQHPEWFVRFENTLYFDPGNKEVQE
                     YVTKVIRDIVHRYDIDALHFDDYFYPYDIVEGGPKGKDFPDNATYARYGNGMTKGDWR
                     RSNVDSVILMISRAIKEEKPYCKFGISPFAIWRNRSQDPEGSDTQGGVTDYDNLYANI
                     LLWLKRGWVDYVVPQIYFDFDHPRAPYGPLLDWWAHHSYGRQCYIGLGIYKAYERIAA
                     WHDPTQIPRQIQAMRTYPEVQGAVYFSSSTFQNNPYGWCDSLQNNYYNYPALIPPMPW
                     LDSTKPHDPLFHIEYDRKDGTGTAWLSKGDAGDSLRGFAIYQSDSATIDIDSVHAYAF
                     IPFDPVAGFTIHRTPAEDKAPVHYYFATAISRNNVESRPV"
BASE COUNT          499 a          640 c          539 g          404 t
ORIGIN      
        1 atttttatgc tggtacacca gctgcgcaga gatttacctt gataatttct gtatttttag
       61 gccagcatgg accaacggtc ccatacaata tggcacttga aaacgaaata aatcagagga
      121 aattccgcac cgaataccag aaagccagga tcaacatcat ctacacctat aattggctct
      181 ctgaaaagat caactccatc ttcgaggaat gggatatcac tccccggcaa ttcaatatcc
      241 tgcgcatcct gcgcggagaa ggaaagccct tgtcgacgct gcagatccgc cagcggatgc
      301 tcgataagat gagcgacacc agccgcatcg tcgaccggct cctgaagaaa ggcctcgtcc
      361 gcaaaacgcc aaacagcggg gaccggcgcc tggtcgacgt ggtgatcacg tcaaaaggaa
      421 aaaagctgct ggagaagatc gaccccttcg aagaccacgc cgataagttc atgaccagtc
      481 tgtccgaaga agaaacgaag acgctgaacg gcctgctcga caagcttcgt aacctgtcct
      541 gcgtgatcct gctgctggtg ctgaccgccg ccggcgtatc cgccccggca acggcgtttt
      601 cccaacctgc cgcatcgaca tcatcactgc ccgccccggt aatgccaaaa tatgagttcc
      661 gcgcagcctg ggtcgcctca gtggagaata tcgactggcc gtcaaagaaa ggacttcccg
      721 tcgacagcca gaaagtggag ttcacccggc tgctggacat gcacaaacga aatggcctga
      781 acgcggtggt ggtccagatc cgccccgccg ccgacgcctt tttcccttct tcctacgaac
      841 cctggagcga atggctcacg ggcgtccagg gcaaggcccc cgacccctgg tacgatccgc
      901 tcagctttat gatcgaagag acccaccggc gcgggatgga atttcatgcc tggtgcaatc
      961 cctatcgcgc cgtaaagacg atcggccggt cctccgttgc cgccgatcat atcacccgcc
     1021 agcacccgga atggttcgtc cgcttcgaga acacgctcta cttcgacccg ggaaataagg
     1081 aagtgcagga gtatgttaca aaggtgatcc gcgacatcgt ccatcgctat gacatcgacg
     1141 ccctgcattt cgacgactat ttttatccct atgatatcgt cgagggcggt ccgaaaggca
     1201 aggactttcc cgataacgcc acctacgccc gctatggcaa cgggatgaca aagggcgact
     1261 ggcgccggag caatgtagac tcggtgatcc tgatgatcag cagggcgata aaagaagaga
     1321 aaccctattg caaattcggg atcagcccct tcgccatctg gcgcaacagg tcgcaggacc
     1381 cggagggcag cgatacccag ggcggagtga cggactatga taatctctat gccaatatcc
     1441 tgctgtggtt gaaaaggggc tgggtcgact atgtcgttcc gcagatctac ttcgacttcg
     1501 accatccgcg cgcgccgtat ggaccgctgc tggactggtg ggcccatcac agctatggcc
     1561 ggcaatgtta tatcggtctc ggcatctata aagcatacga aaggatcgcc gcctggcacg
     1621 atccgaccca gatcccccgg cagatccagg cgatgcggac ctatcccgaa gtacagggcg
     1681 ccgtctattt cagcagcagc accttccaga acaaccccta cggctggtgc gacagcctgc
     1741 agaataatta ttataactac cccgcactga tcccgccgat gccctggctc gactcgacaa
     1801 aaccacacga cccccttttc catatcgagt acgatcgcaa ggacgggacc ggtacggcct
     1861 ggctttccaa aggagacgcc ggcgactcgc tgcggggctt tgccatctat cagtcggact
     1921 cggcgacgat cgatatcgac tccgttcacg cctatgcctt tatccccttt gaccccgtgg
     1981 ccggcttcac catccaccgg acaccggcag aggataaggc gccggttcac tattactttg
     2041 ccacagccat cagccggaac aatgtcgaaa gccggccggt tc
//
LOCUS       DATGFG010000860         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1530759, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000860 DATGFG010000000
VERSION     DATGFG010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530759"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VKQ52_16205"
     CDS             complement(<1..547)
                     /locus_tag="VKQ52_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88799.1"
                     /translation="MLPSRAPLVDAGKDTVLRLADSMLASRRVDTPEYKVSKDTLEAN
                     IEYKASDSIVMIIPTRNITLYSKAETKYKDADLTADHIVYDQDRNVVVARPGLDTAGK
                     PLGLPKMVQTDNTMVSDSIVYNIKSQKGLTHNTITNSGEMFVHAEKMKKITPEEYFGW
                     RGVFTTCDLDTPHFAFRTNKMK"
     gene            920..1843
                     /locus_tag="VKQ52_16210"
     CDS             920..1843
                     /locus_tag="VKQ52_16210"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLZ88800.1"
                     /translation="MIKKTATLLTLCGIVVGLTSLIPVPSPQQPLLRTIVIDPGHGGF
                     DPGTHGLIAKEKDIALAISLQLGKFLQQAYPGLRIVYTRTTDIMPGNKPNLQEGIRYR
                     AELANRSGGDLFICIHCNSSGKAAGSYPVKKLIRYRTVGRGAHKRSVPVYKTAWIKNT
                     VRGTGCYIWKADRDGPKGDAINRREELIHGENMGDSTGTAFDLTSPEAKMRAMLYEKK
                     YFANSALFGSFVEREFVKIGRRSEGLVQRQEGIGVLQATGMPSVLIETGYLTNREEER
                     YLASRKGQAEVARNIATAFKHYKDKVEGLDK"
     gene            1884..>2289
                     /locus_tag="VKQ52_16215"
     CDS             1884..>2289
                     /locus_tag="VKQ52_16215"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLZ88801.1"
                     /translation="MKWYKLSTVLLMGGLYTALTSFTAGGPTPRPHQRNALRTIVIDP
                     GHGGFDTGTKGLQISEKEVALDISLKLGKLIEETWPDVKIVYTRTTDIMPGGGSTIAS
                     GLNYRAELANKSRGDIFIAIHCDNDGHPAGPFS"
BASE COUNT          550 a          684 c          595 g          460 t
ORIGIN      
        1 tcttcatctt gttcgtccgg aacgcgaagt gcggcgtatc caggtcacag gtagtaaaga
       61 ctcccctcca cccgaaatac tcctccggcg ttatcttctt catcttctcc gcatgcacga
      121 acatctcccc ggaattggtg atcgtattat gcgtgagccc cttctgggat ttgatgttat
      181 agacgatgga atcggagacc atcgtattat ccgtctgcac catcttgggt agacccagcg
      241 gcttgccggc cgtatccagc cccggccgcg ccaccaccac attccggtcc tggtcataaa
      301 cgatatggtc ggcggtgagg tccgcatcct tgtatttcgt ctctgccttg ctgtaaagag
      361 tgatattccg ggtggggatg atcatcacta tcgagtcgga agccttgtac tcgatattcg
      421 cttcgagggt atccttcgag accttatatt ccggagtgtc cacccgcctg gacgccagca
      481 tcgagtcggc cagccgcaac accgtatcct tcccggcatc caccagcgga gcccgcgacg
      541 gcaacaccgc ccgcggtaaa atggtgtcgg cccccggccg gaaacgcggt ctcgtggtat
      601 cggcgccggc ccctccgcga ctgccttcct gatcatctcc cctcttccgg gcgccccggt
      661 cggtccccct gccgcccccg gttgttagga ttttgcaaaa acggacaagg cttagataat
      721 ttgctggact attgagcgtt actgagaaga ataaagcagt aaaaatcaat gtcagtaaat
      781 attttgagct aattttgcgt ccgttattgt tcatataggt ataaaaaacg aatagttctc
      841 gggcgtgcgg tgacaaaaat agccatatct cccataagat aatgtgaaga ttaaagggaa
      901 atggcaaaat aacaaaacta tgatcaaaaa gacggccact ttgttaacgc tctgtggaat
      961 agttgttggt ctcacgtctt tgatcccggt gccatcgccg caacaacctt tgctccgtac
     1021 catcgtgatc gatccgggtc acgggggatt cgaccccggc acacacggac tgatcgcaaa
     1081 agaaaaagat atcgcgctgg ccatttccct gcagctgggg aaattcctgc aacaggctta
     1141 tcccggtctc cgtatcgtct atacccgaac gacggatatc atgcccggta acaagcccaa
     1201 cctgcaggaa ggtatccgct accgtgcgga actggccaac cgctccggcg gcgatctctt
     1261 catctgcata cattgcaact cttcgggtaa agccgccggt agctacccgg taaaaaaact
     1321 gatccgctac cgcacggtcg gcaggggcgc ccataaaaga agcgtccccg tctacaagac
     1381 cgcctggata aagaacacgg tccgtggaac cggttgttac atctggaaag ccgatcgcga
     1441 tggcccaaaa ggcgacgcga tcaaccggcg cgaagaactg atccacgggg agaacatggg
     1501 cgacagcacc ggcacggcct tcgatcttac ttccccggaa gcaaagatgc gggcaatgct
     1561 ctatgaaaag aaatattttg ccaatagcgc gctgttcggc agcttcgtgg aaagagagtt
     1621 cgtgaagata ggccgccgca gcgaaggcct ggtacagcgg caggaaggga tcggggtcct
     1681 gcaggccacg gggatgccca gcgtactgat cgagacgggc tacctcacca accgggaaga
     1741 agaacgctat ctcgccagcc ggaaaggcca ggccgaagta gccaggaaca tcgcaacggc
     1801 cttcaaacac tataaggata aggtggaagg cctcgacaaa tgacatggat gcgcccccct
     1861 cggggccgaa atatacgaag agaatgaaat ggtataaact atctactgtg ctcctgatgg
     1921 gcggcctcta caccgccctc acgtccttta ccgccggcgg ccctaccccc cggccacacc
     1981 agcgcaatgc ccttcggacg atcgtcatcg accccggcca cggcggcttc gacaccggaa
     2041 caaaaggcct ccagatcagc gagaaagagg tcgccctcga catctcccta aaactcggga
     2101 agctgatcga ggaaacctgg cccgatgtca agatcgtcta tacccgcacg acagacatta
     2161 tgcccggcgg cggctcgacc atcgcctcgg gactcaacta ccgcgccgaa ctggccaata
     2221 aatccagggg ggatatcttt atcgccatcc actgcgacaa cgacggccat cccgccggtc
     2281 ccttctcgg
//
LOCUS       DATGFG010000861         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_850610, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000861 DATGFG010000000
VERSION     DATGFG010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850610"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VKQ52_16220"
     CDS             complement(<1..1244)
                     /locus_tag="VKQ52_16220"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ88802.1"
                     /translation="MRISFLFLLLFFCGAQLLSARDAVGQDINKVFISLELKDESLLS
                     ALDKIQRLTPFTFAYNKREVKRINNLTMPGSSRSVNSILYTILLHTDLRYEQVGSTIV
                     ISPARAESLPPPMVDETAKEDRSPREGEAEPKSKPITGTVSNERGDPLSGVSITIHGT
                     KTGTTTDASGHFKLTVPDEGATLDLSSVGYQNQSITTGSQTVFNIVMAESIKGLNDVV
                     VIGYGTVRKSDLTGAVASLKGDQLTDRPVPNVSQALEGKIAGVDVSINSNAPGQAAKV
                     RVRGIGSINSSLDPLYVVDGVSGVDINTINPHEIASLEVLKDASSTAIYGARGANGVI
                     MVTTKRGVRANTRVTYEGNVNRSDLYRHLKTLNSEQFIQVYNQAYVNATNFDPLGGTW
                     APPVPLNHIALPKLFDANDKPL"
     gene            complement(1447..2490)
                     /locus_tag="VKQ52_16225"
     CDS             complement(1447..2490)
                     /locus_tag="VKQ52_16225"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ88803.1"
                     /translation="MENSQRLPFLFRRYLEKVCTPEEKEELFALVLRAEYDDTLRQLI
                     AETWNEDHPSYPQDKAHADTILRQILSRREPEPPPDVVSSHTPILTSIFRPRLRTVAF
                     RWVACACTFLLVSTLTFRLFHRSHRASATSIPPAAVAAQYPPVDRCITLPDGSKILLH
                     KTAKLDYQSGFSAKAREVTLHGEAYFDIHKDARPFIVHTGAVRTTVLGTAFNIDANDE
                     RDIVITVTKGKVKVENEKGEYSILHRNEQLSVDSTRLQKLQVNVNEVLSWKKTWLLFN
                     DVPMREAMDELARRYRLTVVFTNPAAENCPVTATFSGEESLDGMINVLSKINNMKYTI
                     DHDRVTIAGDGCK"
     gene            complement(2601..>2928)
                     /locus_tag="VKQ52_16230"
     CDS             complement(2601..>2928)
                     /locus_tag="VKQ52_16230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ88804.1"
                     /translation="REAGFRRQIAGMLETGANPVEDRLLTRENELRLQQALDQLPPRQ
                     KLIFTLSRHQGMKHEEIADHLNISRHTVKTHLVQALKTLRQLLHFPSDGWLPTLLLFL
                     AQFFRS"
BASE COUNT          588 a          718 c          954 g          668 t
ORIGIN      
        1 tacagcggtt tatcgttggc atcgaacaat ttcggcagcg cgatatggtt caggggaacc
       61 ggcggcgccc aggtgccacc cagcggatca aagttcgtgg cattaacata cgcctggtta
      121 tatacctgga tgaactgttc ggagttgagt gtcttgagat ggcggtacag gtccgaacgg
      181 ttgacattcc cttcataggt gacccgggtg ttagcccgga ccccgcgttt ggtggtgacc
      241 atgataacac cattggcgcc gcgggcaccg tagatggccg tggaactggc gtccttcagg
      301 acttcaaggg aggcaatctc atgtgggttg atcgtgttga tatccacacc gctgacgccg
      361 tctacgacat aaagcggatc caggctggaa ttaatggagc cgataccccg gacgcgtact
      421 ttcgccgcct ggccgggtgc attgctgttg atgctgacat ccaccccggc gatctttcct
      481 tccagggcct gggagacgtt gggcacgggc ctatccgtca attggtcacc ctttaagctg
      541 gcgacggccc ccgtgaggtc cgacttcctg accgttccat aaccgatgac cacgacgtcg
      601 ttgaggccct tgatgctctc ggccataaca atgttgaaga ccgtctggga gcccgtcgtg
      661 atggactggt tctgatagcc cacggaggag agatcgaggg tggccccttc gtcgggcacc
      721 gtcagcttaa agtggccgct ggcgtcagtg gttgtaccgg ttttcgtccc atgtatggta
      781 atggagaccc cggagagggg gtcgccccgc tcgttggaga cggtgccggt gatgggcttg
      841 cttttgggtt cggcctcacc ctcccggggg gagcggtctt cttttgccgt ctcgtccacc
      901 atgggaggtg gtagcgactc tgcccgggcg ggggagatga cgatggtact accgacctgt
      961 tcgtaccgga gatcggtgtg gagcaggatg gtatacaaaa tggagttgac cgaccggctg
     1021 ctgccgggca tggtgagatt gttgatccgc ttgacctccc gcttattgta cgcaaaggta
     1081 aaaggcgtga gccgttggat cttgtccagg gcggagagca gggattcgtc ttttagttcc
     1141 agggaaataa agaccttgtt gatatcctgt ccgacggcgt cgcgggcgct tagaagctgg
     1201 gcgccgcaga agaacaggag gaggaaaagg aagctgatcc gcataaaata cagcaatcgt
     1261 tttttggtgt aacagagcag ttgggggcgc cctgtttcca ggcagggacg cgcaaacgct
     1321 gacaatccta aaaatgtcat agttttgatt gttttaaatg acgcaaactt gtttaaaacg
     1381 ggatacctcc ttctcaaaca gaaaggtatc ttttaccact gctccgggtt ccagccggag
     1441 cagtttttat ttacaaccat cacccgcgat cgtgacgcga tcgtggtcga tggtatactt
     1501 catgttgttg atcttcgaca gcacattgat cattccatcc agtgattctt cgccgctgaa
     1561 agtagcggtg accgggcagt tctccgcggc agggttggtg aacacgacgg tgaggcggta
     1621 gcgtctcgcc aattcgtcca tcgcttcccg catcggcacg tcgttgaaca gcagccaggt
     1681 ctttttccag gagaggacct cattgacatt gacctggagc ttctgcaggc gggtggagtc
     1741 cacggagagc tgttcgttcc ggtgcaggat actatactcg cctttctcat tctccacttt
     1801 caccttgcct tttgtaacgg tgatgacgat gtcgcgttcg tcgttggcat cgatgttaaa
     1861 ggcggtgccg agcaccgtcg tgcggacggc gccggtgtgc acgataaagg gacgggcgtc
     1921 cttatggatg tcgaaatagg cctctccatg gagggtcact tcacgggctt tggcactgaa
     1981 gccggattga taatcgagtt ttgcggtttt gtgcagcagg atcttgctgc cgtcgggtag
     2041 ggtgatacag cggtcaaccg gcgggtattg cgcggctacg gcggctgggg gtatggacgt
     2101 agcggaagcg cggtgggacc ggtgaaaaag ccgaaaggtc agggttgaaa cgaggaggaa
     2161 tgtacaggcg caggccaccc agcggaaagc gaccgtgcgg aggcgaggcc ggaagatgct
     2221 ggtgaggatg ggggtatggg aggagacgac atcgggagga ggctcgggtt cccgccggct
     2281 gaggatctgc cggaggatgg tatcggcgtg ggccttgtcc tggggataag agggatgatc
     2341 ttcgttccag gtttcggcga tcagttggcg gagggtatcg tcgtattccg cccgcagtac
     2401 cagggcaaag agttcttctt tttcctccgg ggtacagact ttctcaagat accggcggaa
     2461 caggaagggc aatcgttgtg agttttccat ttcaataagg ttaacagcgg cttgaataag
     2521 gttgacagcg gctgagcaca ggcccgcagc ataaagactg gaaaatgcca ggacaggagt
     2581 agccgggagg aaaatatttt ttaactgcgg aaaaactgcg ccaggaacag gagcagggtg
     2641 ggcagccagc catccgatgg gaagtgcaaa agctggcgca gggtttttag cgcctgtacg
     2701 agatgggtct tgacggtgtg gcgggagatg ttcaggtgat cggcgatctc ttcgtgtttc
     2761 ataccctggt gtctgctgag tgtgaagatg agcttctggc gggggggtaa ctggtcaagg
     2821 gcctgctgca gccgcagttc gttctcgcgg gtgagcagcc ggtcttccac ggggttggcc
     2881 ccggtctcga gcatcccggc gatctgccgg cggaaaccgg cctcccgg
//
LOCUS       DATGFG010000862         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_869605, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000862 DATGFG010000000
VERSION     DATGFG010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869605"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VKQ52_16235"
     CDS             <1..726
                     /locus_tag="VKQ52_16235"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLZ88805.1"
                     /translation="KVYKHVAKDLFVGAGLSFEIRTNIDAGDTAVNATPSGVYNSRHG
                     FPRDHYSANGFLFNLEYITRDNPNRPYNGIYFDAGVRINQTWIGSTKNSVQLTCDFRN
                     YFSLSRASPEFVLALWNWGSYLVSGEIPYLELPGTGRDGTFRSGRGYTTQYFKGTQFN
                     DTEAELRFPILANKFVSGVVFGNLQSGNDAHGTRLFQQFQPGYGAGLRVLFNKATRTN
                     LALDYAWGQFGSKGFFLNLNETF"
     gene            765..>2529
                     /locus_tag="VKQ52_16240"
     CDS             765..>2529
                     /locus_tag="VKQ52_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ88806.1"
                     /translation="MTNPYLSLLSTAWRYARKERKKYLLVYFMFICANLVFSLNPILF
                     GWFIGHLQRDITQVWHYALLYVAGFIGIKFVQWCFHGPARIMERTLAFHLSRNFLQEK
                     YHQVLHLPAKWHQDHHSGSTINRVRKAYEALREFFDRGFMYTYAFSKFIVSVAAILYF
                     SPLYGSIAVVMGIATLLVIRTFDKPFIKALDEVNEREHTVSATLFDSLSNIMTVITLR
                     LEKSMETGLLNKVSRIFRPFRKSALINEWKWFCAEMLIVSIYGLITIGYVHQHWTPGK
                     MFLVGGLVTLLGYVTTFTSVFQDIAWMYTDIITYNTNVQTANLISEAYAEQHRADRSD
                     GLPAGWKTLHISGLNFSYHQLYDRQRGAQSLHNLDITIRRGQRIALVGESGSGKSTLL
                     ALLRGLYVPEPGFDIRLDGRDTIAESDTPQGLLDRVNEAVTLFPQEPEIFENTLAYNI
                     TLGLPFAEEEIMKVCESAHFTEVIRTLPQGLESDIREKGVNLSGGQKQRLALARGILA
                     ARDSDLVLLDEPTSSVDPRTEAMIYEGLFKAFAEKAIISSIHRLHLLSRFDYVYVLHQ
                     GRIVSEGTFEQLRDNDPAFGEM"
BASE COUNT          557 a          823 c          612 g          537 t
ORIGIN      
        1 aaggtataca aacacgtcgc aaaggacctt ttcgtcggcg ccggactctc cttcgagatc
       61 aggacgaaca tcgatgcagg agatacggca gtcaatgcaa ctccctccgg cgtgtacaac
      121 agccgccacg gatttccgcg ggatcattat tcggcgaatg gttttctttt caacctcgaa
      181 tacatcaccc gggacaaccc caaccgaccc tacaacggca tctatttcga tgcgggcgtc
      241 cggatcaacc agacctggat cggcagtaca aagaattcgg tgcagctgac ctgcgatttc
      301 cgcaactatt tcagcctttc gcgagccagc ccggaattcg tgctggcgct ctggaactgg
      361 ggctcctacc tcgtcagcgg ggagatcccc tacctggaac tgcccggtac cggccgggac
      421 ggcaccttcc ggagcggccg cggctatacc acccaatatt tcaaaggcac acagttcaat
      481 gatacggaag cggaactacg ttttcccatc ctggccaata agttcgtcag cggggtcgta
      541 ttcggcaatc tccagtcggg aaacgatgcc catggcacaa ggctgtttca acagttccag
      601 cctggctacg gtgccggcct ccgcgtgctg ttcaacaaag ccacccgcac caatctcgcc
      661 ctcgactatg cctgggggca attcggcagc aaaggattct tcctgaacct aaacgagact
      721 ttttagtccc attcacttcg ccacaaaatc gtaattttag gacgatgacc aatccttatc
      781 tttccctgct cagcactgcc tggcggtatg cccgcaaaga acggaagaag tatctcctcg
      841 tttatttcat gttcatctgc gccaacctgg tcttttcgct aaatcccatc ctttttggct
      901 ggttcatcgg ccacctgcaa cgggatatca cgcaggtatg gcattatgcc ctcctctatg
      961 tcgccggctt catcgggata aaatttgttc aatggtgctt ccacggcccc gcccggatca
     1021 tggagcggac cctggccttc cacctcagcc ggaacttcct gcaggagaaa taccaccagg
     1081 tgctgcacct gcctgcaaaa tggcaccagg accatcacag cggctccacc atcaaccgcg
     1141 tgcggaaagc ctatgaagcc ctgcgggaat ttttcgatcg cggcttcatg tacacctatg
     1201 ccttctccaa gttcatcgtc tccgttgccg ccatcctcta tttctccccg ctgtacggga
     1261 gcatcgccgt cgtcatgggc atcgccacgc tcctcgtcat ccggaccttc gacaaacctt
     1321 tcataaaagc gctcgacgaa gtgaatgaaa gagagcacac cgtctcggca acgcttttcg
     1381 acagcctttc caatatcatg accgtgatca ctctccgcct ggagaaaagc atggagaccg
     1441 gtctcctgaa taaagtatcc cgcatcttcc ggccctttcg caaaagcgcg ctgatcaacg
     1501 aatggaaatg gttttgtgcg gaaatgctga tcgtcagtat ctacgggctg atcaccatcg
     1561 ggtatgtcca ccagcactgg acgccgggaa aaatgttcct ggtcggcgga ctggttaccc
     1621 tgctcgggta tgtcaccacc tttaccagtg ttttccagga catcgcctgg atgtataccg
     1681 atatcatcac gtataacacc aatgtgcaga cggcgaacct gatcagcgag gcctatgccg
     1741 agcaacaccg tgccgatcgc tccgacggac tgcctgccgg ctggaagacc ctccatatca
     1801 gcgggctcaa tttctcctat catcagctat acgaccggca acggggcgcc caaagcctgc
     1861 ataacctgga tatcaccatc cggcgcggtc agcgcatcgc cctcgtcggc gagagcggca
     1921 gcggcaagag caccctcctc gccctgctgc gcggcctcta tgtccccgaa ccgggcttcg
     1981 atatccggct cgacggcagg gatacaatcg cggaatccga taccccacag ggtttgctcg
     2041 accgcgtgaa cgaagccgtc accctcttcc cccaggaacc cgagattttc gagaacaccc
     2101 tcgcctataa tatcaccctc ggtttgccct ttgccgaaga agagatcatg aaagtctgcg
     2161 agagcgccca tttcaccgaa gtgatccgga cgctgccaca gggactcgaa tccgatatcc
     2221 gcgaaaaggg cgtcaacctc tccgggggcc agaagcaacg cctcgccctg gcccggggga
     2281 tcctcgccgc ccgtgacagc gacctcgttc tcctcgacga accgaccagc agcgttgatc
     2341 cccgaacgga ggccatgatc tacgaaggcc tcttcaaagc cttcgccgag aaggccatca
     2401 tttcctccat ccaccgcctc cacctgcttt cccgcttcga ctacgtctat gtccttcacc
     2461 agggccgcat cgtttccgaa ggcactttcg aacaactccg cgacaacgac ccggcctttg
     2521 gcgagatgt
//
LOCUS       DATGFG010000863         7371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_783475, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000863 DATGFG010000000
VERSION     DATGFG010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783475"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VKQ52_16245"
     CDS             <1..506
                     /locus_tag="VKQ52_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88807.1"
                     /translation="PNYSLTVADYDAQVGFLLDVRNKYNEVQKAILRIRNIRTQLQDL
                     NGRLDSTEKPVRKLSDSLIRELTSIEEALYQTKSKSAQDMLNYPIRINDKLSGVYGIA
                     AGGNSVPSKQAREVFATLRGLADRQLARLQLVVQTGLPDLNRMIYEKQIPVISVKEKE
                     TSTSGNP"
     gene            531..1121
                     /locus_tag="VKQ52_16250"
     CDS             531..1121
                     /locus_tag="VKQ52_16250"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HLZ88808.1"
                     /translation="MENNWLDLSSTLGLIATCVLTFNLLLGILLSTAYRRSPLWKRLP
                     DWIKKISLDDLHNWTAYVALALALAHPVLLLPDATLRYRIATILFPVDAPHQAFWTVL
                     GSLALYALVVVIITTQKAVKRRLGFRAWKNIHLISYGTALLFVIHGIVMDPELKDRPI
                     DWLDAEKLVSELCGLILLVATVIRWRYYQRYQRRLT"
     gene            1229..4390
                     /locus_tag="VKQ52_16255"
     CDS             1229..4390
                     /locus_tag="VKQ52_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88809.1"
                     /translation="MRRRLFGLLLGFTILHASAQSFDSSLFRQLHFRFIGPDGNRAIA
                     VAGVPGDRNTAYVGAASGGIYKTEDAGITWRPIFDSMDNSSIGALAIAPSDPRQVWAG
                     TGETFLIRPAEAMGNGVYKSVDAGRTWTNSGLQATGRISRVIVDPTDTNIVYVAALGN
                     THAPQQERGVYKTTDGGKTWERVFFVDPYTGCADLAIDPRHPGTLYAAMWQITFNTWQ
                     LNSGGPGSGIFKTTDGGKTWTRLNNGLPGGAVHPVGKTSVDVSYSNPNIVYSLIEDKD
                     PALYRSEDGGASWKKMFTSHSMAQRASYYTRVRVSTGDPDNVFTICVTVMESKNGGKS
                     FNGNGNGDYRPGGDTHDMWFDPKDPSRAMVAHDGCMNMTYNGGKTWDNVNLPIAQMYH
                     VAVDNQIPYFVYGNRQDGSSYRGPSNSLQGGIPVSLWTTIGGCESGYAQPDPVDNNII
                     WSGCYDGGLDRTDLRTGHVRDVRAWPEAGYGYPPAEMRYRWHWNYPMAVSRHDHNKVY
                     IGSQYVHVTTNEGQSWTVISPDLTTNDKSHQQSSGGVSTDNLFTFDGCTLYVIAESPV
                     QEGLLWTGSNDGLVHLTRDDGKHWEDVTANIPGLPKWSTIRSIDPSGFDAGTAYISVD
                     AQFIGDFNPYIYKTTDFGRTWTLISGSFPKSNSSFVHQIKEDPGKKGLLWAGTDNGLY
                     FSPDDGANWIHLKNNLPPVPVYGIAIQPNFKDLVLGTYGRGFYILDDITPIRDLSTQV
                     QASDAWLFPLRKAYRFRPKISIHGERSTNTGQNPPYGADINYYLKSDIKDTVRLLVLD
                     AAGNTVQQIKGSNRRGINRVWWDLGLQPYVLPPLRTRPEDADWVKLDSNGERAMVIYD
                     LDIGPGLSTTKVLPGNYTVVLKVGDTEYRQPVEVLRDPHTVGTEEDIREQYTFGVQIY
                     QSIQGVLHMIDTLEILRGKADSIGARLVNKKQRAKLLAWQKQLVEVEGLLHDVHQTGA
                     RQDIFRNPAQLLERFLTISKESISGGSDYRPTDQHREVYSLLSGRLVTARTKYEATLK
                     SFPAPLPAGLLPREKLDKQQKNN"
     gene            4401..4931
                     /locus_tag="VKQ52_16260"
     CDS             4401..4931
                     /locus_tag="VKQ52_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88810.1"
                     /translation="MKKTGAILLLLLLFYTQWGHYAQFVLLQWRMREAARETWVAALP
                     DTAFLRVSLADMNAGGKWEEAGKECWYKGHLYDVIRTKTGKGTTILFCMDDEREERLI
                     RQSGEVTRANQGQDQDHGNPKTGHSLPFSIGDLVCEGADWYIRPIPNLPRRYATGGYN
                     CLPTLYTEIVLPPPKG"
     gene            4977..7115
                     /locus_tag="VKQ52_16265"
     CDS             4977..7115
                     /locus_tag="VKQ52_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ88811.1"
                     /translation="MLKKTGIFLLHMLVLSAVKAQHNPKDTVGKVQTLNSVQVTSWSA
                     GRKDNQLDVPRSVGILTAEDFHRNSGLSLENSLNLMPGVSMGSRTSFGGQRIIIRGYG
                     NNTNFNGQGVQVLLNNIPVTDATGTTIMDDIDFSTLGKVEVIKGPASSLYGSGIAGVV
                     NLFTMKPQPNQGRIVEENTGGSNGLWRNNTRIETANGNSALLFNYGHQEEQGWRANSA
                     SRKDYAQFSGDFFVSDKNTLSAYFAYAHSLEQLAGEMTDSIFYHRIKWSDPAYLVNGS
                     KTVIESFRAGVTDNYTIDRHFSNLTTVYTNTYTLNQPFAHGLTDNQAFTFGGRTGFVY
                     TSDTGTVTVHGILGAQFQKTIAFNKSYNLTNGVLGGIRGDLQNSSQGYNIFTEWKFSL
                     PAQVILTAGGSLDFTEYAIADMLANSANPTHANQSGDKHFSPVFMPRISLLKMLNDHV
                     SFYADISRGYTPPPTADVINAVLGTANRNLKPESGIQYEIGSKGDLFDRKLSYQLALF
                     DLDVHNKLVAQQVPASGSTPTYTTYVNAGEQQDIGLELALSYTLLDNKNETVSLLRPF
                     VSYTWSDFTYKDFYSDNNHNAATLDFSHNKVAGVAPNVVNAGIDLQTRMGFYAYLTWR
                     HVDAVPYTFDNVNYARAYNLLNGKIGYRSQLGDHFSVDLYAGVDNLTNSTYYSFLFFS
                     GTLAGTTDPHFLPMPYTAAWYGGAKIAYIF"
     gene            7127..>7371
                     /locus_tag="VKQ52_16270"
     CDS             7127..>7371
                     /locus_tag="VKQ52_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88812.1"
                     /translation="MRKTGYCIWAVLLLGACSPFGDKHNDRKGLRIVSVSKQLTEIIF
                     AVGGDTALVGRDISSTWPPAAMRITAVGYHRALNPEG"
BASE COUNT         1657 a         2151 c         2062 g         1501 t
ORIGIN      
        1 atcccaatta ttcgctcaca gtagctgatt atgacgcgca ggtgggattc ctgctggatg
       61 tccgcaacaa atacaatgag gtgcaaaagg ccatcctccg catccggaat atccggacgc
      121 agttgcagga cctgaacggc cgcctcgaca gtaccgaaaa accggtgcgg aaactatcgg
      181 atagccttat ccgtgagctg acctccatcg aagaggccct gtaccagacc aaatcgaaaa
      241 gcgcacagga catgctcaat tacccgattc ggatcaacga taagttgtcc ggggtttatg
      301 gcatcgccgc cggcggtaat tccgtgccca gtaaacaggc gcgggaagtt tttgccaccc
      361 tgcgcggcct tgccgaccgg caattggccc ggttgcagct ggtggtgcaa accggcctgc
      421 ctgacctcaa ccggatgatc tatgaaaaac agatccccgt catcagcgta aaagaaaaag
      481 aaacctccac ttccggtaat ccttagagcg cccgaagcca aataaaagat atggaaaaca
      541 actggcttga cctttcttcg acccttgggc tcatcgccac ctgtgttttg acctttaacc
      601 tgctgctggg catcctgctc agcacggcct accgccggag tccgctgtgg aagcggctgc
      661 cggattggat aaaaaagatc agcctggacg accttcataa ctggacggcc tatgtggccc
      721 ttgcccttgc actggcgcac ccggtgttgc tgctgcccga tgcgacgctg cgttaccgga
      781 tcgctacgat ccttttcccc gtcgacgctc cacaccaggc cttctggacg gtgcttggct
      841 cccttgcgtt gtacgcgctg gtggtggtca tcatcacgac gcagaaagcg gtgaagcgcc
      901 ggctggggtt ccgggcatgg aagaatattc atctcatctc ctatggtacg gccctgctct
      961 ttgtcatcca tggcatcgtg atggaccccg aactgaagga ccggccgatc gactggctgg
     1021 atgcggagaa gctggtatcc gagctttgcg ggctgatcct gctggtggcg acggttatcc
     1081 gctggcgcta ttaccagcgt taccagcgcc ggctaacctg aaatcagccg ccggcattca
     1141 ggcaggattt taaccaatgc ttcatcgatt tgaacgcgcg gtagcattgt tgacgatcct
     1201 aaggtaaatt tcctaaaata atcgtgctat gagaagaaga ctatttgggt tactgttggg
     1261 attcactatc ctgcacgctt ccgcccaatc cttcgacagc agcctcttcc ggcaactgca
     1321 tttccggttc atcggccccg atggcaaccg cgccatcgcc gtggcgggtg tcccggggga
     1381 cagaaatacc gcctatgtgg gcgcggcctc gggcggcatc tataaaacgg aagatgccgg
     1441 tattacctgg cgtcccattt tcgacagcat ggataattca tccatcgggg cgttggcaat
     1501 cgcgccctcc gatccgcggc aggtgtgggc cggcacgggg gagacctttc ttatccgccc
     1561 cgcggaagcc atggggaatg gggtgtataa gtccgtcgac gcgggcagga catggaccaa
     1621 tagcggattg caggcaacgg ggaggatcag ccgcgtcatc gtcgatccca cggataccaa
     1681 catcgtatac gtagccgcgc tggggaatac ccatgcgccg cagcaggagc ggggtgtcta
     1741 taagacaacc gacggtggca aaacctggga gcgcgtcttt tttgtcgatc cttataccgg
     1801 ctgcgccgat ctcgccatcg atccccggca tcccggaacc ctttatgcgg ccatgtggca
     1861 gatcacgttc aacacctggc agctcaacag cggcggaccg ggcagcggca tcttcaaaac
     1921 cacggacggc gggaagacct ggacccggct gaacaatggc cttcccggcg gcgccgtcca
     1981 tcccgtgggc aagacatcgg tggatgtatc ctacagcaac cccaatattg tctattcgct
     2041 gatcgaggac aaggaccccg ccctctaccg cagtgaggac ggcggggcct cctggaagaa
     2101 gatgttcacc agccactcga tggcgcaacg ggccagctat tatacccggg tgcgcgtgtc
     2161 gacgggcgac cccgataacg tctttaccat ctgcgtcacg gtcatggagt cgaagaacgg
     2221 cggcaaatcc tttaacggca acgggaacgg cgactatcgt cccggcggcg acacccatga
     2281 tatgtggttc gacccgaagg accccagccg cgccatggtc gcccacgatg gttgtatgaa
     2341 catgacctat aacggcggca agacctggga caacgtcaac ctgccgatcg cccagatgta
     2401 tcatgtggcg gtggacaacc agatcccgta tttcgtctat ggcaaccggc aggacggcag
     2461 cagctaccgt gggcccagca acagcctgca gggcggtatt cccgtttccc tgtggacgac
     2521 gatcggcggt tgtgagagcg gctatgccca acccgatccc gtggacaaca atatcatctg
     2581 gtcgggttgt tatgacggcg gtctcgaccg gaccgatctg cgcaccggtc atgtccggga
     2641 tgtccgtgcc tggccggagg ccggctatgg ctatccgccg gccgagatgc gatatcgctg
     2701 gcattggaac tacccgatgg ccgtttcccg gcatgatcac aacaaagtgt atatcggcag
     2761 tcagtatgta cacgtcacta ccaacgaagg gcagagctgg acggtcatca gccccgatct
     2821 gacgaccaat gacaaatccc atcagcagag ttccggcggg gtcagcaccg acaacctgtt
     2881 cacctttgac ggatgtaccc tctatgtgat cgccgaatcc ccggtacagg aaggcctttt
     2941 atggacgggc agcaacgacg ggctcgttca tctcacccgc gatgacggca aacactggga
     3001 agatgtcacc gccaatatcc ccggcctccc taaatggtct acgatccggt cgatcgaccc
     3061 ctccggcttc gacgccggga cggcttatat ctccgtcgac gcccagttca tcggcgactt
     3121 caacccctat atctacaaaa cgaccgactt tggcaggacc tggacgctga tcagcggcag
     3181 ctttccgaaa agcaattcgt cctttgtcca ccagataaag gaagacccgg gtaaaaaggg
     3241 attgctttgg gccggcaccg acaacggact gtatttctct cctgatgacg gagctaactg
     3301 gatccatctc aagaataatt taccacctgt tcctgtttat ggcatcgcca tccagcccaa
     3361 ttttaaagac cttgtgctgg gaacctatgg ccggggcttt tatatcctgg acgatataac
     3421 gcccatccgt gatctcagca cccaggttca ggcttccgac gcctggctct ttcccctgcg
     3481 gaaagcgtac cggttccggc cgaagatcag catccatggc gaacgttcca ccaacaccgg
     3541 gcaaaacccg ccctatggcg ccgatatcaa ctattacctg aagtcggata taaaggacac
     3601 ggtcaggctg ctggtactcg atgcggcggg gaatacggtg caacagataa aaggatcgaa
     3661 ccgtcgcggc atcaaccggg tctggtggga tctgggcctc cagccctatg tactgccgcc
     3721 gttgcgcacc cggcccgagg acgccgattg ggtaaagctg gactccaacg gggagcgggc
     3781 gatggtgatc tatgacctgg atatcggtcc cggcctctct accaccaagg tgctccccgg
     3841 gaattatacg gtcgtgctga aagtagggga taccgaatac cgccagcccg ttgaggtgct
     3901 ccgcgacccg catacggtgg ggacggagga ggatatccgg gaacagtata cctttggcgt
     3961 acagatctac cagtcgatac agggcgtgtt gcatatgatc gatacgctgg agatcctgcg
     4021 cggcaaggcc gattccatcg gggcaaggct ggtgaacaag aaacagcggg caaagctgct
     4081 ggcctggcag aaacaattgg ttgaagtgga aggcctgttg catgacgtac atcagacggg
     4141 cgcccgccag gacatcttcc gtaacccggc ccagctgctg gaacgctttc tcaccatctc
     4201 gaaagaaagc atctccggcg gttcggacta caggcccacg gaccagcacc gggaggtgta
     4261 cagcctgctg agcggccggc tcgtcaccgc gcgtacgaaa tacgaggcta ccctgaagag
     4321 ctttcccgca ccgctgcccg cgggtctgtt acccagggag aaactggata aacaacaaaa
     4381 aaacaactga cagccctggt gtgaaaaaaa ccggcgccat attactcctg cttctgctgt
     4441 tctataccca atggggacac tatgcccagt tcgtccttct tcagtggcgg atgagagagg
     4501 ctgcccggga gacctgggtc gccgcgctcc ccgatacggc ctttttgcgg gtgagccttg
     4561 ccgacatgaa tgccggcggc aaatgggagg aagccgggaa agaatgctgg tataagggac
     4621 atctttacga cgtgatccgg acaaagaccg ggaaggggac caccatcctg ttctgtatgg
     4681 atgatgaacg ggaggaacgc cttatccggc aatccggtga agtgacaagg gcgaaccagg
     4741 ggcaggacca ggatcacggt aacccgaaga ccgggcattc cctgcctttt tccatcggcg
     4801 accttgtctg cgagggcgcc gattggtata tccgcccgat ccccaacctc ccgcgacggt
     4861 atgcaaccgg cggatacaac tgtctgccga cgctctatac ggagatcgtc cttcctccgc
     4921 caaagggata gcctgttctt cttcatcatc gaaagtcatt accaaaacgt attatcatgt
     4981 tgaaaaaaac aggaatattc ctattgcata tgcttgtcct gtccgctgtg aaggcccagc
     5041 acaacccaaa agacacggtg ggaaaggtgc agaccctcaa ttcagtccag gtgacgagtt
     5101 ggtccgccgg caggaaagat aaccagctgg atgtgccccg gagcgtgggt atcctcaccg
     5161 ccgaggactt tcatcgcaac agcggattgt cgctcgagaa ttcccttaac ctcatgcccg
     5221 gggtgagtat gggttcccgg accagctttg gcggccagcg catcatcatc cgcggctatg
     5281 gcaacaatac caacttcaac ggccagggcg tccaggtgct gctgaacaat atcccggtga
     5341 ccgatgccac gggaaccacc atcatggacg atatcgattt ttccacgctc ggcaaggtgg
     5401 aggtgatcaa gggtcctgcc tcgagtttgt atggcagcgg catcgccggt gtcgtcaatc
     5461 tctttacgat gaagccgcaa cccaaccagg gccgtatcgt ggaagagaac acggggggca
     5521 gcaacgggct ctggcgtaac aatacccgca tcgaaacggc caacggcaat tcggcccttc
     5581 tcttcaatta cggtcaccag gaggagcaag gctggcgggc caacagcgcg tcccggaaag
     5641 actatgccca gttcagcggc gatttttttg tcagcgacaa gaacaccctt tccgcctatt
     5701 ttgcctatgc ccattcgctc gagcaactgg cgggagaaat gacggatagt atcttctacc
     5761 accggataaa atggagtgac ccggcctatc ttgtcaatgg cagcaagacc gtgatcgaga
     5821 gtttccgtgc gggggttacc gataactata ccatcgaccg gcatttcagc aacctgacga
     5881 cggtgtatac caacacctat acgctgaacc agccctttgc tcatggccta accgataacc
     5941 aggcctttac ctttgggggc cgcaccggtt ttgtctatac ctccgacacg ggtacggtca
     6001 ccgtgcatgg catcctcggc gcgcagttcc agaagaccat cgccttcaac aaatcctaca
     6061 acctgaccaa cggggtgttg ggcggcatca ggggcgacct gcaaaattcc tcgcaggggt
     6121 acaatatatt cacggaatgg aaattcagcc tccccgcgca ggtgatcctc accgccggcg
     6181 gcagtctcga cttcacggaa tatgccatcg ccgacatgct ggccaattcc gccaatccca
     6241 cccatgccaa ccaatctggg gataagcatt tttcccccgt gttcatgcca aggatctccc
     6301 tgctgaagat gctgaacgat catgtctcgt tctatgccga tatcagccgg gggtatacgc
     6361 cgccaccgac ggcggatgtc atcaacgcgg tactggggac ggcgaatagg aacctgaaac
     6421 ccgaatcggg tatccagtac gagatcggct cgaagggcga cctttttgac cgtaagctct
     6481 cctatcagtt ggcgctcttc gacctggacg tccacaacaa gctcgtcgcc cagcaggtac
     6541 ccgccagcgg cagcacacct acgtatacta cctatgtgaa cgcgggtgaa caacaggata
     6601 tcggtctgga actggcgctt agttacacgt tgctggacaa taagaacgag actgtctcgc
     6661 tgttgcggcc ctttgtctcc tacacctggt cggattttac ctacaaggat ttttacagcg
     6721 acaataacca caatgccgca acactcgatt tttcccataa caaggtggcg ggggtagccc
     6781 ccaacgtggt gaacgcgggt atcgatctgc aaacgaggat gggtttttat gcttatctta
     6841 cctggcgtca tgtggatgcg gttccgtaca cgttcgacaa tgtgaactat gcgcgggcct
     6901 acaacctgct caacgggaag atcggctacc ggagtcagct gggggatcat ttctcggtgg
     6961 acctgtatgc cggagtggac aacctgacga acagcacgta ttacagcttc ctgttcttct
     7021 cgggtacgct tgccgggacg accgatccgc actttctgcc gatgccgtac accgccgcct
     7081 ggtatggcgg ggcgaagatc gcgtacatct tttaatgtat tgagctatga gaaaaacagg
     7141 ctattgtatc tgggcggtgc tgctccttgg cgcctgcagt ccgttcgggg ataaacacaa
     7201 tgacaggaag gggctccgca tcgtcagcgt cagcaagcag ctcacggaga tcatctttgc
     7261 cgtgggcggc gatacggcgc tggtagggcg ggatatttcc agcacctggc ctcccgcggc
     7321 gatgaggata acggcggtgg ggtatcaccg ggcgttgaac ccggaaggga t
//
LOCUS       DATGFG010000864         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_275145, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000864 DATGFG010000000
VERSION     DATGFG010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275145"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VKQ52_16275"
     CDS             <1..664
                     /locus_tag="VKQ52_16275"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ88813.1"
                     /translation="ILQGYIRRDPRLVLEGACRTAPETLEKLNNGTIDLLFLDIQLPD
                     ISGIRLLQNLTPAPLVIFTTAFREYALDAFELNAVDYLLKPFSFDRFLQAVNKAAELL
                     QTRQEVRADREKAPFFIRDGTKLILLPLEDILYAEALREYVRIVTATKQHVVHISMKG
                     LEQKLPAGQFVRIHKSYIISVSRITAIEGGTVTIGGRRLPISRQDRNEVLEKITKNRF
                     IG"
     gene            760..1857
                     /locus_tag="VKQ52_16280"
     CDS             760..1857
                     /locus_tag="VKQ52_16280"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HLZ88814.1"
                     /translation="MKSTFCLLLLPLLLIAWPRERLLRLAPLHEKRASHSATLLPDGR
                     VLIAGGFRKGPDTYSQLYSNTAELYDPIRHTFTYTGNFQVARCGHTATLLANGKEVLI
                     TGGNNDHPRLASAELYDIPTGRWIRLPDMLAGREGHQAVLLNNGKVLIIGGTGDLRTS
                     VELFNPATRKFEKSEPAPVDLNGTALPLADGRILIAGGNQDRQPTQFAITYDPKTNRF
                     TRVGDMTVVRYKSSAALLPDGRALIIGGSNNRDWRGKYSSTELFDLRTNTFTRGPELN
                     FERFKLAHSIITLKNGDILVTGGDRHIEQLHDGRFSVVATLDQPCYFSTATLLNGGDI
                     LLAGGYGNDAQCIDKTWIYTPQVTTMPLSSR"
     gene            complement(1824..>3225)
                     /locus_tag="VKQ52_16285"
     CDS             complement(1824..>3225)
                     /locus_tag="VKQ52_16285"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HLZ88815.1"
                     /translation="APLFDLHAPAIYIESIEFLLMIVLTFVLKKHLSRYLFYGWCIFL
                     FLFLLLPITRILGLPLSMQRWAELFIDLASLAFGSFYVFSKRRMGGQLRFVYAAAGLY
                     LLLNLLAVICNLFGRVTLSQIFGATAVYSFAQTVSLAVFVKLIIEAFLLQIIASRVRK
                     GYPETFDLAAVTKSLYRFAILLAAVLWLIVLSTNLNLFDALNDWLTDFFSASREVGNF
                     SFTIGGLVLFLGIIWLANFLQRFIAYFFGDTGDDAAMDDRGQRSRLLITRLVLLIGGF
                     LLAVAASGLSVDRITVILGALGVGVGLGLQNIVNNFVSGIILIFDRPVRIGDTVEIGD
                     KRGRVKEIGIRSSTLLTDEGAEVIIPNGDVLSSRVVNWTLSNNNVRLVLTFTIEKPAN
                     PEAIEPDTIKAIVHQHPNVLERRDPEVLVNSVSSKTEEIKVFFWIKDIAKAPFTTGEI
                     RTSIYRHLDDKGIVVT"
BASE COUNT          746 a          961 c          900 g          618 t
ORIGIN      
        1 catcctgcag ggctatatcc ggcgcgatcc gcggctggtg ctggaaggtg catgccggac
       61 ggcgcccgag accctggaga agctaaacaa cgggaccatc gatctcctct tcctcgatat
      121 ccagctcccg gacatctcgg gtatccgtct gctgcagaac ctgacgccgg cgccccttgt
      181 catcttcacc acggctttcc gggaatatgc cttggacgcc ttcgagctca acgccgtcga
      241 ctatctcctg aagccttttt ccttcgaccg cttcctgcaa gccgtcaata aggcggctga
      301 actgttgcag acccggcagg aagtccgggc cgatcgggaa aaagctccct tcttcatccg
      361 tgacggaaca aagctgatcc ttcttccgct ggaagatatc ctctacgcgg aggccctgcg
      421 ggaatatgtc cgcatcgtca ccgcgacaaa acaacatgtg gtgcacatct cgatgaaggg
      481 attggagcag aagctgcccg cgggacaatt cgttcgcatc cataaatcct atatcatctc
      541 ggtatcccgg ataacggcca tcgaaggcgg aaccgtcacc atcggcggcc ggcgcctgcc
      601 gatcagccgc caggacagga atgaggtcct tgaaaagatc acgaagaacc gttttatcgg
      661 gtagccgcag ggcttccatt cgtgtgtctt ttcccgcagt tggtagaata ttttttcccg
      721 ccccgcacca cccttctacc ttgaacaaaa atcagcgata tgaaaagcac gttctgtctc
      781 ctcttactac cgctcctgct gatcgcctgg ccaagggagc ggcttctgcg gctcgctccc
      841 ctgcatgaaa aacgcgccag ccacagcgcc accctgctgc ctgacggccg cgtcctgatc
      901 gccggcggtt ttcgcaaagg ccccgacacc tatagccagt tatatagcaa caccgcggaa
      961 ctctatgatc ccatccgtca caccttcacc tataccggaa atttccaggt cgcccgttgc
     1021 ggacatacgg ctaccctgct ggccaacggc aaagaggtgc tgatcaccgg cggcaataac
     1081 gaccatcccc ggctggccag tgcagaactc tacgatatcc cgacgggccg gtggatccgg
     1141 ctgccggata tgctggcggg ccgtgaaggt caccaggccg tactgctgaa caacggaaaa
     1201 gtgctgatca tcggcgggac cggcgatctc cggacctcgg tggaactctt caacccggcg
     1261 acgcgaaaat tcgagaaaag cgaaccggct cccgtcgacc tcaacggtac ggcgctgcca
     1321 ttggcggatg gccgtatcct gatcgccggt gggaaccagg atcgtcagcc aacacagttt
     1381 gcgatcacct atgacccgaa gaccaatcgc tttacccggg tgggcgatat gacggtggta
     1441 agatacaaat cctcggctgc cctgttgccg gatggccggg ccctgatcat cggcggatcg
     1501 aataaccgtg actggcgcgg gaaatacagc agcaccgagc ttttcgatct ccggacaaac
     1561 acctttaccc gtgggcccga gctgaatttc gaacgcttca agctggccca ctccattatc
     1621 acgctgaaga atggggatat cctcgtcacc ggtggcgacc ggcatatcga gcaactgcac
     1681 gacggccgtt tctccgtcgt cgccaccctc gatcaaccct gttatttttc taccgcgacc
     1741 ttgctgaatg gcggggatat cctgctggcc ggtggctatg ggaatgacgc gcaatgcatc
     1801 gacaagacct ggatctatac tcctcaggtg acgacaatgc ccttatcatc gaggtgacgg
     1861 taaatgctgg ttcggatctc gccggtggtg aagggcgctt tggcaatgtc ctttatccag
     1921 aaaaacacct tgatctcctc ggtcttcgag ctcacggagt tgaccaggac ttccgggtcc
     1981 cggcgttcca gtacattggg atgctgatgc acgatggctt tgatcgtatc aggctctatc
     2041 gcttcgggat tggcgggttt ttcgatggtg aaggttagca ccaggcgcac gttgttattg
     2101 ctaagggtcc agttgacgac gcggctggaa agaacatccc cattaggaat aatgacttcc
     2161 gcaccctcat cggtgagcag ggtgctgctg cggatgccga tctccttcac gcggccccgc
     2221 ttgtctccta tttcgaccgt atcgccgatc cgcacgggcc ggtcgaagat caggatgata
     2281 cccgatacaa agttgttgac gatattctgc agacccaggc cgacgccgac gccaagggcg
     2341 ccgaggatca ccgtgatccg gtcgacggaa agtcccgatg ccgctaccgc cagcaggaaa
     2401 cccccgatca gcaggacgag ccgggtgatc agcagccggg atcgctgtcc gcggtcatcc
     2461 atggcggcat cgtccccggt gtcgccaaag aaataggcga taaaccgctg caggaaatta
     2521 gccagccaga taatgcccag gaagagaaca agtccgccaa tggtgaacga gaagttgccg
     2581 acctcccgcg atgcgctgaa gaaatcggtg agccagtcat tcagggcgtc gaaaaggttc
     2641 aggttggtgc tgagaacgat cagccagaga acggcggcca gtagaatggc aaaccggtac
     2701 agggatttgg tgaccgctgc caggtcgaag gtctccggat atcccttgcg cacccggctc
     2761 gcgatgatct gcaacaagaa ggcctcgatg atcagtttga cgaacacggc cagcgacacg
     2821 gtctgcgcaa aggaatacac cgccgttgcg ccaaagatct gcgagagggt cacccggcca
     2881 aaaagattac agatcacggc cagcaggttc agcaacaggt aaaggcccgc cgccgcatag
     2941 acaaagcgaa gctgaccgcc catccgccgt ttgctgaaga catagaacga cccaaaggcc
     3001 agggaggcca ggtcgatgaa cagttcggcc cagcgctgca tcgaaagcgg caacccaagg
     3061 atgcgggtaa tgggtaatag cagaaagagg aacaggaaga tacaccagcc atagaaaaga
     3121 taccgcgaaa ggtgcttctt caggacaaag gtcagcacga tcatcagcag gaattcgatc
     3181 gactcgatat aaatagccgg cgcgtgcaga tcgaacaagg gggcc
//
LOCUS       DATGFG010000865         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_386544, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000865 DATGFG010000000
VERSION     DATGFG010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386544"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VKQ52_16290"
     CDS             <1..836
                     /locus_tag="VKQ52_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HLZ88816.1"
                     /translation="ACWIPALKARRLVLAGDHCQLPPTIKSEEAARGPQGAGGAGVAA
                     GTRIAGLGVTLLEKLVGRYPEAVVLLEEQYRMHTSIMGYSSELFYQQRLQAHPTVAGH
                     TLFEGDTPLAFIDTAGCGYDESPEGTGLTNPEEAAFLVRHATQLVKELATRYSAADFP
                     TLAVISPYRSQVQLLQDLVRQTEGLKAYLDKIAVNTIDSFQGQERDIVYIGMTRSNPD
                     NRIGFLSEIRRMNVAMTRARKKLVVIGDSATLSRQPFYAGLIAYAERLEAYQSAWEFV
                     E"
     gene            complement(865..2457)
                     /locus_tag="VKQ52_16295"
     CDS             complement(865..2457)
                     /locus_tag="VKQ52_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88817.1"
                     /translation="MRNFLFLFLGLAVGSNAFSQEQTWLGSRPAPVLLESFDGLGEGF
                     TGPQGTALYRNPSDNALAVGPDHIVQIVNSRMAIYTKKGKRFDTTGRVLFGPGETRSV
                     FKGFGGPCERLNNGDAVVRYDQLANRWLIVMPTFRRGIPRTQELDSAHGMAPYPPQPG
                     KAEMLYQAPPVYAEERPASNGARPPRTRIDTIGTYCMCYAVSTGSDPLGPYYRYEFDR
                     PLFPDYPRPAVWPDGYYTTTSTSDDLIQRQAFVADREKMLKGEPATEQSFIVNGVNFL
                     LNSDVEGQRLPARGAPNIMVTNGGTQLKNNFEDDGIYFWKFRVDWKDTTKTKLEGPVR
                     ITVAPYHYAGDGQLKKSVPQPGTDMRLDTQGDKLLGRLIYRRIGKQESYIVVHSVRTA
                     AGGSGVRWYEFRSGKHHRPGLYQQGTFAPDSDWRWLPGIATDRYGDMGIGYSYGGSAH
                     YPGQRFTGRLAGDSLGILPFGEGILAEGQAAQTNTLRWEDYTYMVVDPSDDATFWYVG
                     DYLKKDAKNYSTRIGAFRLTRP"
     gene            2579..3046
                     /locus_tag="VKQ52_16300"
     CDS             2579..3046
                     /locus_tag="VKQ52_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88818.1"
                     /translation="MKPFLPILLPGAVLIFALAALSALSACHKGAKKPTIPTPLYSQW
                     QWVSYSIPPGPNGNELRPAKDSLVLLFMDADSNFNIRINDSLVSAGYYKISADSTDMY
                     LTPAALLYPPDSLRLCTACSLSIVGDTLRLRAPFANPTNYGTFTFAKPRVYAD"
     gene            complement(3071..>3494)
                     /locus_tag="VKQ52_16305"
     CDS             complement(3071..>3494)
                     /locus_tag="VKQ52_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88819.1"
                     /translation="AMDHLSILPLEARDYLTLSGGERQRVQFARALAQIWDPPATGCR
                     YLFLDEPVSSLDIHYQHEFLGLTRRLAASGTVIIAVLHDLNLALQYAHRMLFLKEGRV
                     VAAGKAPEIVTPEIIHEVFGVSAHLLENPYGQRPVIVY"
BASE COUNT          712 a         1071 c         1003 g          708 t
ORIGIN      
        1 ctgcctgctg gatcccggcg ctgaaagcaa gacggctggt ccttgccggc gatcactgcc
       61 agctgccgcc gacgatcaaa tccgaagaag ccgcacgtgg accgcaaggc gcgggggggg
      121 caggagttgc agcgggtaca aggatcgccg ggctcggtgt aaccctgctg gaaaagctgg
      181 tgggccgcta tcccgaagcc gtcgtcctgc tggaggaaca ataccggatg catacgagca
      241 tcatggggta ttcttcggag ctgttctatc agcagcggct acaggcgcat cctaccgttg
      301 ccggtcatac gcttttcgaa ggggatactc ccctcgcttt tatagacacc gccggctgtg
      361 ggtatgacga gagtcccgaa ggcacggggc ttaccaaccc ggaggaggcc gcctttctcg
      421 tacgccatgc gacacagctg gtaaaggagc tggccacccg ttactccgcc gccgacttcc
      481 ccaccctcgc cgtcatctcc ccctatcgca gccaggtgca gctgttgcag gaccttgtgc
      541 ggcagaccga ggggttgaag gcctatttgg ataagatagc ggtaaatacg atcgacagct
      601 ttcagggaca ggagcgggat atcgtctata tcgggatgac ccggagcaac ccggacaacc
      661 gtatcggttt cctgtcggag atccgccgga tgaatgtcgc catgacaagg gcgcgaaaaa
      721 aactcgtcgt catcggcgac agcgcaaccc tgtcccggca gcctttttac gccgggctta
      781 tcgcctatgc cgagcggctg gaggcatacc agagcgcctg ggaatttgtg gaataaatgg
      841 ccgcttcgca tggagtaaat gcgcttatgg acgggtaagc cggaaggcgc cgatccgggt
      901 gctataattc tttgcgtcct tcttcaggta atccccgaca taccagaacg tggcgtcgtc
      961 cgacgggtcg accaccatat acgtatagtc ttcccagcgc agggtattgg tctgcgccgc
     1021 ctgcccctcg gcgaggatgc cttccccaaa aggcaggatg cccagggaat cccccgcaag
     1081 gcggccggtg aaacgctgac cgggatagtg tgcgctcccg ccataggaat agccgatgcc
     1141 catatcccca tacctgtccg ttgcaatgcc cggcagccag cgccagtcgc tatccggcgc
     1201 aaatgtcccc tgctgataaa ggccaggccg gtgatgtttc ccgctccgga actcatacca
     1261 gcgcaccccg ctgccgcccg cggcggtcct gacggagtgg acgacgatat aggattcctg
     1321 tttgccgatg cgccggtaga tcagtctccc cagcagtttg tcgccctggg tgtcgagacg
     1381 catatcggtt cccggttgcg gtaccgactt ctttaactgc ccgtccccgg catagtgata
     1441 gggcgccacg gtgatccgca ccgggccttc cagtttcgtt tttgttgtat ccttccagtc
     1501 gacacggaat ttccagaaat agatgccgtc gtcctcgaaa ttattcttca actgcgtgcc
     1561 gccgttagtg accatgatat ttggcgcgcc cctggcaggc agtcgctgcc cttcaacatc
     1621 ggagttgagc aggaaattga caccattgac gataaaggat tgttcggtgg cgggctctcc
     1681 cttcagcatc ttttcgcggt cggcgacaaa ggcctgacgc tggatcaggt cgtcactggt
     1741 gctggtggtc gtataatacc cgtccggcca gacggcaggc cgtggatagt cggggaacaa
     1801 gggccggtcg aattcatagc ggtaataagg accaagcggg tcggatcccg tgctgacggc
     1861 atagcacata cagtaggttc cgatcgtatc gatccgggta cgcggcggtc gggcgccgtt
     1921 ggaggcgggc cgttcttccg cgtagaccgg cggcgcctgg tagagcattt cggctttccc
     1981 gggttgcggg ggatagggcg ccatgccatg ggcggaatcc agctcctgtg tacgggggat
     2041 gccgcgacgg aaagtgggca tcacgatcag ccagcggttg gccaactggt cataccgcac
     2101 cacggcatcg ccattgttta gccgctcgca ggggccgcca aaccctttga agacactgcg
     2161 ggtctcgccg gggccgaaaa gcacccggcc agtcgtgtcg aagcgttttc ctttcttcgt
     2221 atagatcgcc atccgggagt tgacgatctg gacaatatgg tcgggtccca cggcaagggc
     2281 attgtccgaa ggattgcggt agagagcagt accttgcgga ccggtaaagc cttctcccaa
     2341 cccatcaaag ctctcgagca gcaccggcgc cggccgcgac cccagccagg tctgctcctg
     2401 ggaaaaagca ttgctcccca cagcaaggcc aaggaagagg aataagaagt ttctcatgtt
     2461 taaatttccc gaaacaaacc gaaacccgct tttctaaaat tatccgttcc ccataataaa
     2521 ttaaccggtt tgcaacgttc atccaaaaag cattgtcagg gcagtaaacc gacgcgacat
     2581 gaaacctttc cttccgatcc tgctgccagg cgccgtcctc atattcgccc tggccgcgct
     2641 atccgcctta tccgcctgcc ataaaggggc taaaaagcca accatcccta ctcccctata
     2701 ctcccaatgg cagtgggtca gttacagcat cccacccggg cctaacggca acgagctgcg
     2761 tcccgcaaaa gactcccttg tcctcctttt catggatgcg gactccaact tcaatatccg
     2821 catcaacgac tccctcgtgt ccgccggata ttataagatc agtgccgatt ccacggatat
     2881 gtatctcacc cccgcggccc tgctgtatcc ccccgacagc ctgcggctct gtaccgcctg
     2941 ttccctctcg atcgtcggcg acaccctccg gttgcgcgcg ccgtttgcca accccaccaa
     3001 ttacggcacc ttcacctttg ccaaaccgag ggtctatgcg gattgaatgg ccgcgtgaag
     3061 ccaggtgttg ttaataaacg atcacgggtc gctgcccata cgggttctcc agcagatggg
     3121 cggacacgcc aaagacctcg tggatgatct ccggtgttac gatctccggc gctttgccgg
     3181 ccgcgaccac ccggccttct ttcaggaata acatccggtg ggcatactgc agcgcaaggt
     3241 tgagatcgtg cagcacggcg atgatcaccg tccctgaagc cgccagcctg cgcgtcagtc
     3301 cgaggaactc gtgctggtaa tggatatcga gactggacac cggttcgtcg agaaacaaat
     3361 aacggcagcc cgtggcaggc gggtcccaga tctgggccag ggctcgcgcg aactggaccc
     3421 tttgccgttc gccgcccgag agcgtaagat aatcccgggc ttcgagcggc aggatgctga
     3481 ggtgatccat ggcc
//
LOCUS       DATGFG010000866         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_60450, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000866 DATGFG010000000
VERSION     DATGFG010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60450"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VKQ52_16310"
     CDS             complement(<1..434)
                     /locus_tag="VKQ52_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88820.1"
                     /translation="MKKFLFPTMFACCLLVHARAQQDPWLNPTLSIDVRVKDLIGRLT
                     PQEKVYQMMNSTPAIPRLHIPEYDWWNEALHGVARSGIATIFPQPIGMAATFDDGLVH
                     QVADAISDEARAMYNAAIKKDYRPRFAGLTFWTPNINIFRDPR"
     gene            565..1821
                     /locus_tag="VKQ52_16315"
     CDS             565..1821
                     /locus_tag="VKQ52_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HLZ88821.1"
                     /translation="MSLHLRAGNLSSCLILSFALTLFSCHKQVSAPAVDTPLNLPPGS
                     TAVTSSTNQFAFSLLHSILQHEPTGANELISPFSIYMALSMTYNGAAGPTRDSMAVAL
                     QQSGIPVSQLNTVSQALISQLPKEDSKVQLSVANAIWYAKNIAQPSSTFLNTIQQDYL
                     GTMQSLDFTQPASVNTINTWVAKNTNNKIPAILDQVPSNTAMVLVNAVYFNGVWRNAF
                     DPNQTRDMPFTLADGNTISTPFMTREMTIRANFGPSFAIAELPYSTGKGFNMYLVVPN
                     DKNRSITDFAASFNLDTLTSAWARLDSTPVGLWIPKWEYAYSIPNLKTELTALGMGLA
                     FGNADFSNMFSGESVNISQVAHKAYIKVSEEGTEAAAATGVVVRITASPVIPALFADH
                     PFLYLITEKQTGTVLFIGFVTNPSVN"
     gene            complement(1860..3311)
                     /locus_tag="VKQ52_16320"
     CDS             complement(1860..3311)
                     /locus_tag="VKQ52_16320"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HLZ88822.1"
                     /translation="MIRSGWTALLLLWMMFSVVGVFAQIRVPRMVSDGMVLQRDMPLR
                     IWGYASPGEKVTVKFAGETASGVTDDNGKWVVQLSPKKAGGPYNMDIDGINHIWLKNI
                     LVGEVWVVAGQTGMAMPMERVKAKYADLIAHADNPSIRQFLVTMRYDFKAPRENTPAG
                     NHWEPATSTSILSFSALGYFFARELYERYHVPIGLINVCAPDAPAEAWLSKDGLRAFP
                     EYAAVAARYADSSFRDGTGPADRMAPGGLFNGMIAPVCMHTVRGIIWDQGEADLGKAP
                     EYAGLFPLLINDWRRHWDEGALPFLYVQAPAYGPAKETPGESQWADLREAQRMAMALP
                     ATAMIVATDLGDVTDVPGQNREELSKRLSLAAESIAFGKRNFISTGPLYHSMKVHGDR
                     VHINFTEVNTGLVVKDGGELKGFAVAGDDGRFLPAKASIDGKTVEVWSEQVPHPVAVR
                     YAWADNPAGANLYNRDILFKDGLPAPPFAARAK"
     gene            complement(3358..>4187)
                     /locus_tag="VKQ52_16325"
     CDS             complement(3358..>4187)
                     /locus_tag="VKQ52_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88823.1"
                     /translation="FGAARPAGDPPAAQQLIADGWGYVLIDPGSIQADNGAGLTRGII
                     GLVNKGQPRKPDDWGALRAWAWGAARALDYLEANEPMVDSRHVGIEGVSRYGKAALVT
                     LAFEPRFAMGLIGSSGEGGAKLHRRNWGEAVESLTGGEYYWMAGNFMKYGASDAVFGE
                     KTAKDLPVDAHELIALCAPRLAFISYGVPEQGDARWLDHQGSYMAAVAAGPVYRLLGA
                     RDMGVSGDYTTEKMPPVNTGLLDGRLAWRQHDGGHTDAPNIKFFIEWADKNIGHQAK"
BASE COUNT          937 a         1413 c         1006 g          831 t
ORIGIN      
        1 cgcggatcgc ggaagatatt gatattgggt gtccaaaaag tcagcccggc aaaccgcggc
       61 cggtaatctt tttttatcgc ggcattatac atcgcccgcg cttcatcgga gatggcatcg
      121 gccacctggt gcaccagccc gtcgtcgaaa gtcgcggcca tcccgatcgg ctggggaaag
      181 atggtggcga tgccgctgcg ggcgacacca tgcagggcct cgttccacca gtcgtattcg
      241 ggtatatgta accgggggat ggcgggagtg ctgttcatca tctggtaaac cttctcctgc
      301 ggcgtcagcc gcccgataag gtccttcacc cggacatcga tagataaagt gggattaagc
      361 cagggatcct gctgggccct ggcatgcacc aaaagacaac aggcaaacat cgttggaaaa
      421 aggaattttt tcatggcaga gctgctgatt ttcaggaaaa gtaaccaaaa ttatccaaaa
      481 aatgcaaccg attacatttt tatccgctgc accgcatgca acggccccgc ttcctttctt
      541 gtcaacttcc aaaaccagca acatatgagt cttcacctgc gcgcgggaaa tctttcctcc
      601 tgccttattc tatcttttgc ccttaccctc ttctcctgcc ataaacaggt ctcggcaccg
      661 gccgtcgata ccccgctgaa cctgccaccc ggcagcaccg ccgtcaccag ctccaccaac
      721 caattcgcct tttcgctctt acatagcatc ctgcaacacg aacccaccgg tgcaaacgaa
      781 ctcatctccc cctttagcat ctatatggcg ctgagcatga cctataacgg cgccgccggt
      841 cctacccgcg actccatggc cgttgccctg caacagtcag gcatccccgt gagccagctg
      901 aacaccgtca gccaggccct gatcagccag ctgccaaagg aagacagcaa agtacagctc
      961 tcggtcgcga atgccatctg gtatgcaaaa aacatcgcac aaccctcctc caccttcctg
     1021 aatactatcc aacaggacta tctcggcacg atgcagtcgc tggacttcac ccagcccgca
     1081 tcggtgaaca ccatcaacac ctgggtagca aaaaatacaa ataacaagat cccggccatc
     1141 ctcgaccagg taccctcgaa cacggccatg gtcctcgtca acgccgtgta cttcaatggc
     1201 gtctggcgca acgccttcga tcccaaccag acccgcgata tgccctttac cctggcggac
     1261 ggcaacacga taagcacgcc gttcatgacc cgggagatga ccatccgggc aaattttggt
     1321 cccagtttcg cgatcgccga attaccctac agcaccggca aaggtttcaa catgtatctc
     1381 gtggtcccca acgataagaa ccggtcgatc accgactttg ccgcctcctt caacctggac
     1441 accctgacca gcgcctgggc ccggctggat tcgacaccgg tgggcctgtg gattcccaaa
     1501 tgggagtacg catattcgat ccctaacctt aaaaccgaac tcaccgcgct gggcatgggc
     1561 ctcgccttcg ggaacgccga tttctccaat atgttctccg gcgaatccgt aaacatctcg
     1621 caggtggccc acaaagccta tataaaagta tcggaagaag gaacagaagc agcggcagcc
     1681 accggagtgg tcgtgaggat aacggcgtca ccagtcattc cggccctctt cgccgaccac
     1741 ccctttctct acctgatcac ggaaaaacaa accggtacag tcctgtttat aggatttgtc
     1801 accaatccat cggtaaactg atcccgcctc gagggtggca gctgaaatcg gacagcccct
     1861 tacttcgccc gggcggcgaa cggcggggcc ggcaacccat ctttaaaaag aatatctcta
     1921 ttgtataaat tcgcgcccgc gggattatcc gcccaggcat atcgcaccgc cacgggatgg
     1981 ggtacctgtt cactccagac ctccaccgtc ttcccgtcga tgctcgcctt cgccggaaga
     2041 aaacgcccat catccccggc aacggcaaac cctttcagct cgccgccatc cttgacgacg
     2101 agacccgtgt tgacctcggt gaaattgata tggacccggt ccccatgtac cttcatggaa
     2161 tgatacagcg gaccggtgga gataaaattc cttttcccaa atgcgatgct ctccgccgcc
     2221 agtgacagcc gcttgctcag ctcttccctg ttctgccccg ggacatcggt cacatcgcca
     2281 agatcggtgg ccacgatcat cgccgtcgcc ggcaacgcca tcgccatccg ctgcgcctcc
     2341 cgcagatcgg cccactggct ttcccccggt gtttccttcg cggggccata ggccggcgcc
     2401 tgcacataca ggaacggcag ggcgccttca tcccaatgcc gccgccagtc gttgatcagc
     2461 agggggaaca agcccgcata ttccggggcc ttccccaggt cggcctcgcc ctgatcccaa
     2521 atgatccccc tgacggtatg catacagaca ggcgcgatca ttccattgaa caacccgccg
     2581 ggcgccatcc ggtcggccgg acccgtaccg tccctgaaac tgctgtccgc ataacgggca
     2641 gcaacagccg catactcggg aaaagcccgc aggccgtcct tgctcagcca ggcctcggcg
     2701 ggcgcgtcgg gcgcgcagac attgatcagc ccgatgggaa catgatagcg ttcgtacagc
     2761 tcccgggcaa aaaaataccc cagggccgag aaggataaaa tcgatgtgga agtggcaggc
     2821 tcccaatggt tacccgccgg cgtattttcc cgcggggcct tgaaatcata gcgcatcgtc
     2881 accaggaact gcctgatcga gggattatcg gcatgcgcaa taagatcggc atattttgct
     2941 ttcacccgct ccatcggcat cgccatcccc gtctgcccgg ccactaccca cacctcgccg
     3001 acaaggatat tcttcagcca gatatgattg ataccatcta tatccatatt gtaagggccg
     3061 ccggccttct tcggggacaa ctgcaccacc catttcccat tgtcatccgt aaccccgctg
     3121 gccgtctcac cggcaaattt caccgtaacc ttctcccccg gcgacgcata cccccagata
     3181 cgaaggggca tatcccgctg caaaaccatc ccatcgctga ccatcctggg tacccggatc
     3241 tgggcaaaga ccccaacaac ggagaacatc atccataaca acaataaggc tgtccatcct
     3301 gatctgatca tgagaaccga tttgtaccgc aatatcggtt tttggccgta tattccatca
     3361 tttcgcctgg tgcccgatgt tcttgtccgc ccactcgata aagaatttta tattcggcgc
     3421 atcggtatgt cctccatcat gctgccgcca cgccaaccga ccatccagca atccggtgtt
     3481 caccggcggc atcttttcgg tggtatagtc acccgacact cccatatccc tggcgcccag
     3541 taaccggtaa acaggacccg cggctaccgc cgccatatag ctgccctggt gatcgagcca
     3601 ccgcgcatca ccctgttccg gaacgccata actgataaag gcgagccggg gagcgcataa
     3661 cgcgatcagc tcatgggcgt ctaccggcag gtccttcgcc gtcttctccc caaagaccgc
     3721 atcggaagcc ccatatttca tgaaattccc tgccatccag taatactccc caccggtgag
     3781 gctttccact gcttcacccc agttacgccg gtgcagcttg gcgccgccct cgccggaaga
     3841 gccgatcagc cccatggcaa agcggggttc gaaagcaagg gtcaccagtg cagcctttcc
     3901 atagcgggag acgccttcga tcccgacatg cctgctgtcg accatcggct cattcgcttc
     3961 gagataatcg agcgccctgg cagcccccca ggcccaggca cgaagcgcac cccagtcatc
     4021 cggcttccgc ggctgaccct tattcaccag gccgatgatc ccccgcgtaa gccccgcgcc
     4081 attatccgcc tgtatgctgc ccggatcgat gagaacatac ccccatccat cggcgatcag
     4141 ctgctgcgcg gcaggcggat caccggcggg acgcgccgca ccaaaag
//
LOCUS       DATGFG010000867         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_294621, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000867 DATGFG010000000
VERSION     DATGFG010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294621"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..986)
                     /locus_tag="VKQ52_16330"
     CDS             complement(105..986)
                     /locus_tag="VKQ52_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ88824.1"
                     /translation="MRKPSREIISPAKDASFYMTHFKAPALCHEDYWHFHPEFEIVYV
                     PHGKGKRFIGHRASRYMGGDLVLLGPNILHNTFNFGFESPAATYEEYVIQFKREQIET
                     MTDYFPEFAGVAKLLKKAETGIAVTGKAKHRIGGLIKKMYGLGPFPRLMQLFGVLGEM
                     SLTADTRDLRARESLPLSAAHSSRIQQVYQFIQTRYQDEVATRQIAGHLAMTESSFCR
                     FFKQATGKTLKQALTEVRIHKARLLLTGSDLSIAAVAAHAGFNNVSLFNRVFKLLTNN
                     TPRQYRESMQVSLLVDG"
     gene            1087..>2070
                     /locus_tag="VKQ52_16335"
     CDS             1087..>2070
                     /locus_tag="VKQ52_16335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ88825.1"
                     /translation="MKKNAPIAYRSAVIGYFFVNGFLFSNLTSRLPELKDHFGVSNSV
                     LGTLLFTTAIGALVAMPISGWLSVRFGSARMVIVTSLLFCCCVPCIAFSPNLWLGRAG
                     FFMTGFFSGAMDITMNAQAVLVERIYKKTIMSSFHAAFSIGMALGAGSGALFADLHIP
                     LQGHLVAMAAGCIVLLAAMIPILLEHRQGGAANPVATAGSRRFPLAIWLIAIIGFCSM
                     TGEGSMVDWSAIYTHTVVGMTKAFSALAMGAFATAMTIGRLFGDRLIDRLGKHTILTI
                     SCGLAIGGLGLLLGWVSAPTALLGFFFVGAGLSNIVPLTYSIAGNIPGIDPT"
BASE COUNT          392 a          526 c          663 g          489 t
ORIGIN      
        1 ggggattggc aaggcggcgg ccagcgtggc ggaaccgccg agcaggagga attcgcggcg
       61 ggagaatcgt ttttgcatct caaaatatac tgaaaaatcg gcaatcaacc atcgaccagg
      121 aggctcacct gcatcgattc gcggtattgg cggggtgtgt tgttggtgag gagcttgaag
      181 acgcgattga aaagggatac gttgttgaag ccggcatgag cagcgacggc ggcgatggaa
      241 agatcggagc cggtgaggag cagacgggcc ttatggatac gcacctcggt gagggcctgt
      301 ttgagcgtct tgccggtggc ttgtttgaag aaacggcaga aggagctttc ggtcatggcc
      361 agatggccgg ctatctgccg ggtggcgacc tcatcctggt agcgggtctg gatgaactgg
      421 tacacctgct ggatacggct gctgtgggcc gcggagagcg ggagggattc gcgggccctg
      481 agatcccggg tgtcggcggt gagcgacatt tccccgagga ccccaaacag ctgcatcagg
      541 cgggggaagg gcccaagccc gtacattttc tttatcaggc cgccgatgcg atgttttgct
      601 ttccccgtga cggcgatgcc ggtttccgcc ttcttgagca gttttgcaac gccggcgaat
      661 tcagggaaat agtccgtcat cgtttcgatc tgttcccgtt tgaactggat gacatactct
      721 tcatacgtgg cggcagggga ttcaaagccg aaattaaagg tgttgtgcag gatattgggg
      781 ccgagcaaga ccaaatcgcc gcccatgtaa cgactggccc gatggccgat gaaccgttta
      841 cccttcccat gcggcacata gacgatctcg aactcgggat gaaaatgcca gtagtcctca
      901 tgacagaggg caggagcctt gaagtgcgtc atataaaagg acgcgtcctt ggcgggggag
      961 atgatctcgc gacttggttt acgcatacac cggtttatct gttatcgaca atttaggaca
     1021 atttagtgga aattcctgca aaacggctgg caggtttgtt gacgggcggt tacgaaattt
     1081 gccctcatga agaagaatgc acccatcgcc tatcgtagcg ccgtcatcgg gtattttttt
     1141 gtcaacggct ttttgttttc caacctcacc tcccgcctcc ccgaactgaa ggatcatttc
     1201 ggcgtgagca acagtgttct gggaaccctg ctttttacca cggcgatcgg ggcgcttgtc
     1261 gccatgccga tcagcggatg gctttccgtg cgattcggca gtgcgcggat ggtgatcgtt
     1321 acgtcgttgc ttttctgttg ttgtgtcccc tgtatcgctt tttcacccaa tctctggctg
     1381 gggcgggccg gtttctttat gacgggcttt ttctccggcg ccatggatat caccatgaat
     1441 gcacaggcgg tgctggtgga aagaatatat aagaagacca tcatgtcctc gttccatgcg
     1501 gccttcagca tcggcatggc gctgggggcg gggtcggggg cgttgttcgc ggacctgcat
     1561 atcccgttgc agggacacct ggtggcgatg gccgccggct gtatcgttct gctggcggcg
     1621 atgatcccga tcctgctgga gcatcggcaa ggcggggccg ccaatcccgt tgccacggcg
     1681 ggctcgcgcc gttttccgct ggcgatctgg ctgatcgcca ttatcgggtt ttgcagtatg
     1741 acgggagaag gatcgatggt cgactggtcg gctatttata cgcatacggt ggtaggaatg
     1801 acgaaggcgt ttagcgccct ggccatgggt gcgttcgcta cggcgatgac gatcgggcgg
     1861 ctcttcggcg accgcctgat cgacaggctg ggaaaacaca cgatcctgac catcagttgt
     1921 ggcctggcca ttggggggct tgggctgttg ctgggctggg tctccgcgcc cacggcgttg
     1981 ctgggattct tttttgtcgg agcggggctt tcgaatatcg tgcccctcac ctactcgatt
     2041 gcaggaaata tcccgggcat cgatccaacg
//
LOCUS       DATGFG010000868         4989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_487839, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000868 DATGFG010000000
VERSION     DATGFG010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487839"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..689)
                     /locus_tag="VKQ52_16340"
     CDS             complement(174..689)
                     /locus_tag="VKQ52_16340"
                     /inference="COORDINATES: protein motif:HMM:NF042765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HLZ88826.1"
                     /translation="MAASLFKWFGVVFFSLIFAGTGAGGLHPLYISVTEMNHNSKDKI
                     LEVSCKIFTNDLEAALEKMSGSHVDLSSAKDKAASDKLIGDYVVKHLRLKLDGKAAVL
                     HFVGSEKENDGTWSYFQVNDVPAVKRIDAVNEILYDSFNQQINIMHVTVGGQRKSTRL
                     DYPESSASFEF"
     gene            768..1358
                     /locus_tag="VKQ52_16345"
     CDS             768..1358
                     /locus_tag="VKQ52_16345"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HLZ88827.1"
                     /translation="MQDFKLYFGIGIEHILNPEALDHILFVAALCLRYLMKDWKKVVV
                     LVTAFTIGHSVTLALSALGLVHVATRWIEFLIPLTIVATALNNLAQRTNRVEHPSRLP
                     LIYFFALFFGLIHGLAFASSLLSLVGREGLVVPLLAFNLGIEAAQLVVVAAILLISFI
                     FVRGIRIRRIWWIRGASALVLLASLKMAVDRWPSRL"
     gene            1395..3833
                     /locus_tag="VKQ52_16350"
     CDS             1395..3833
                     /locus_tag="VKQ52_16350"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLZ88828.1"
                     /translation="MKNKLLIGPQFFAAILLLGIGAAGTLQAQDIHNNPTSNHGNKFE
                     ALGTILPTPNEYRTASGAPGPKYWQQRCDYDITCSLDEDKQWLTGSETITYFNNSPNE
                     LTYLWMQLDENEHSSKNNANYQDGSSMPKIVSDQMLDRLSGSKDPNDYGDNIVKITDA
                     LGKSLQYMINKTMMRVELPTPLKPGQKFVFKVDWNYHISDRFKLGGRGGYEYFPEDGN
                     YLFTMTQWYPRLCVYSDFQGWQNNQFTSRGEFALTFGNFKVQMTVPADHIIGATGECQ
                     NYQQVLTPAQFARWQQAQTTKEPVEIVTLAEAKTSEAKKNKAKKTWIFKADNVRDFAW
                     GSSRKFVMDAMPAYVEGKKVMCMSYYGKEAYNLYRRFSTKAVAHTIETYSHFTIPFPY
                     PVAQSVEAANGMEYPMICFNYGRTEKDGSYSEATKYGMLGVVIHEVGHNFFPMIINSD
                     ERQWSWMDEGLNSFVEYLTEELWDNHFPVRGKGPAWAIVDYMKLPKDQLEPIMTNSEN
                     IINFGPNAYTKPATALNILRETVMGRELFDYSFKEYARRWAFKHPTPADLFRTMNDAS
                     AENLDWYWRGWFYGIEPVDLSLDSVKHLTPDFSHVPAHTDSTVVKNIDKPMPDGKFPT
                     DVSKVRNREDKNIVFPTDADTSLHDFYWKYTRGQIPYDTTKYTVQSTFINGREVGGFT
                     NEPLDDAEKTKLQSKQFYELQFGNKGGLVMPIIIEWTYKDGTKEIERIPAQVWRKNES
                     HLTKLFIKDKEVASIKLDPNRETADIDESNNSWPVVQTESKFELFKAKQLGPRAIPQT
                     NNPMQLTGKDKKAF"
     gene            complement(3901..4317)
                     /locus_tag="VKQ52_16355"
     CDS             complement(3901..4317)
                     /locus_tag="VKQ52_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88829.1"
                     /translation="MGAVHAEIELINGTDFQMARRHVIGEDEVRSIRVRMLVDSGSYL
                     MAINENIQAYLDLPIQYKQPAVLADGRVMDCDVVGPIEVRFANRRATCNAFVLPEDCE
                     PLLGAIPMEEMDVLIDMQRQELVVNPAHPDCAILRL"
     gene            4439..>4989
                     /locus_tag="VKQ52_16360"
     CDS             4439..>4989
                     /locus_tag="VKQ52_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HLZ88830.1"
                     /translation="MKKLVPILFTLAILITCVTTRTFAGPVDTILVHSAAMNKDIKCV
                     VISPGGKPAPGAHWPVIYLLHGYSGDYAQWIHTAPQLAQESTADGILFVCPDGGYDSW
                     YFDSPVDPSVRYETFITSELIPYIDRHYSTLPDKTHRAITGLSMGGHGALYLAIRHRD
                     LFGAAGATSGGVDFRPFPDNWGI"
BASE COUNT         1261 a         1561 c         1153 g         1014 t
ORIGIN      
        1 gtttatcctc agcgaactgg aacgcattcc caccacggga gaaaagctcg agtggcgtgg
       61 ctttacattc gagatcatcg atatggacgc cgcccgcatc gacaaggtcc tggtgaccct
      121 gtccgcggag atgcaacggg aattaaagga agaagaggaa gaatgacccc ctgctaaaac
      181 tcgaaggagg cgcttgactc gggataatcc agccgggtgc tcttccgctg ccccccgacc
      241 gtgacatgca tgatattgat ctgctggtta aagctgtcat agaggatctc attgacggca
      301 tcgatgcgct tcaccgccgg tacatcattc acctggaaat agctccaggt gccgtcattc
      361 tccttttcgc ttcccacaaa gtgcaggacc gctgccttcc cgtccagctt taaccggaga
      421 tgcttcacca cataatcccc gatcaatttg tccgaggccg ccttatcctt tgccgaggag
      481 agatcgacat ggctccccga catcttttcc agggccgcct ccaggtcatt ggtaaagatc
      541 ttacaactga cttccagtat cttatccttg gagttatggt tcatctccgt gacgctgatg
      601 tacaggggat gcaggccgcc ggcgcccgtt cccgcaaaaa tcaaagagaa aaacaccacg
      661 ccaaaccact taaataaaga tgccgccatt taaaaagtaa atttcgatag tacaaaagtc
      721 tgaataattt tacaatttcc agcaaacgac catctcccgc acacaatatg caggatttta
      781 aactctactt tggcataggc atcgagcata tcctcaaccc ggaagccctg gatcacatcc
      841 tcttcgtggc ggccctttgt ctgcgttacc tcatgaaaga ctggaaaaaa gtagtcgtcc
      901 tggtcacggc ctttaccatc ggacactccg tcaccctggc ccttagcgcc ctgggactgg
      961 tgcacgtcgc cacccggtgg atcgaattcc tgatcccgct gaccatcgtc gcgacggccc
     1021 tgaacaatct ggcgcaacgg acaaacaggg tggaacaccc ttcccggttg cccctgatct
     1081 attttttcgc ccttttcttc gggttgatcc acggcctggc cttcgccagt tccctgctca
     1141 gcctggtggg cagggaagga ctggtggtgc ccctgctggc gtttaacctg ggtatcgaag
     1201 ccgcccagct cgtcgttgtc gccgccatcc tgttaatttc ctttattttc gtgcgcggta
     1261 tcaggatccg caggatctgg tggataagag gcgcatccgc gctggtgctg ctcgcttcgt
     1321 tgaaaatggc cgttgatcgc tggccatcac ggttgtaagt attgttataa gtattattat
     1381 caaaacatac acacatgaag aacaaactgc ttattggtcc gcaatttttt gctgccatcc
     1441 tgttattggg tatcggtgcc gccggaacgc tacaggcaca ggatatccat aacaatccca
     1501 cctccaacca cggcaacaag ttcgaagcgc tgggaaccat cctcccgaca ccgaacgaat
     1561 accgtacggc aagcggagcc ccgggtccga aatactggca gcaacgatgc gactacgaca
     1621 tcacctgttc cctcgacgag gacaaacagt ggctcaccgg ttcggagacc attacctatt
     1681 tcaacaattc ccccaatgaa ctgacctatc tctggatgca gctcgacgag aacgaacaca
     1741 gctcgaagaa caacgccaac taccaggatg gaagcagcat gccaaagatc gtcagcgacc
     1801 agatgctcga ccgcctgtcc ggctcaaagg accccaacga ctacggcgac aatatcgtaa
     1861 agatcaccga cgccctgggc aaatccctcc aatacatgat caataagacc atgatgcgcg
     1921 tggagctgcc tacccccctg aaaccgggac agaaatttgt ctttaaggtg gattggaact
     1981 atcatatctc cgatcgcttc aaattgggcg gccgcggcgg ctatgaatac ttccccgagg
     2041 atggcaatta tctctttacc atgacccaat ggtacccccg gctttgcgtc tacagcgatt
     2101 tccagggctg gcagaataac cagttcacca gccgcggcga atttgccctc accttcggga
     2161 atttcaaagt gcagatgacc gtccccgccg accatatcat cggcgcaacc ggcgaatgcc
     2221 agaactacca gcaggtcctc acccccgccc agttcgcccg ctggcaacag gcacagacga
     2281 cgaaggagcc ggtagagatc gtgacgctcg cagaagcgaa gacgtcagag gcaaagaaga
     2341 acaaggcaaa aaagacctgg atcttcaaag ccgacaacgt ccgcgacttc gcctggggct
     2401 cttcccggaa attcgtcatg gacgccatgc ccgcgtatgt ggaagggaaa aaagtgatgt
     2461 gtatgagcta ttatggcaag gaagcctata acctctaccg ccgcttctcc accaaggccg
     2521 tggcccatac catcgagacc tattcccatt ttaccattcc cttcccctac cccgtagccc
     2581 agagcgtgga agccgccaac ggaatggaat acccgatgat ctgtttcaac tacggccgca
     2641 ccgaaaaaga cggctcctat agcgaagcca ccaaatacgg gatgctgggc gtcgtcatcc
     2701 atgaagtggg ccataacttc ttccccatga tcatcaacag cgacgaacgc cagtggagct
     2761 ggatggacga aggcctcaac tccttcgtgg aatacctcac cgaagagctc tgggataacc
     2821 acttccccgt tcgcggcaag gggccggcct gggcgatcgt ggattatatg aaactgccaa
     2881 aggaccagct ggagccgatc atgaccaact ccgagaatat catcaacttc gggcccaatg
     2941 cctataccaa gcccgccacc gccctcaata tcctccgcga gacggtcatg ggccgggaac
     3001 tcttcgacta ttcatttaag gaatatgcaa gacgctgggc cttcaaacac ccgacgccgg
     3061 ccgacctgtt ccgcaccatg aatgacgcca gcgcggaaaa cctcgactgg tactggcgcg
     3121 ggtggttcta tggcatcgaa cccgtcgacc tctccctcga cagcgtcaaa cacctgacac
     3181 ccgacttcag ccatgtcccc gcacacaccg actccaccgt ggtcaaaaac atcgacaaac
     3241 ccatgcccga cggcaaattc ccgacggatg tctccaaggt aaggaaccgt gaggacaaga
     3301 acatcgtgtt ccccaccgac gcggatacct ccctgcatga tttctattgg aaatataccc
     3361 gcggccagat cccgtacgac acgacgaaat acaccgtcca gtctaccttc atcaacggca
     3421 gggaagtcgg cggctttacc aacgaaccgc tggatgacgc cgaaaagaca aaactccagt
     3481 cgaagcaatt ctatgaactg cagttcggca acaagggcgg cctggtcatg cccatcatca
     3541 tcgagtggac ctacaaggac ggcacaaaag agatcgagcg cattccggca caggtctggc
     3601 gcaagaacga gagccatctc accaaactct ttataaagga taaggaagtc gccagcatca
     3661 agctcgaccc caaccgcgag accgccgaca tcgacgaaag caacaacagc tggcccgttg
     3721 tccagacgga gagcaaattc gaattgttca aggcaaaaca actcggcccc cgtgccatcc
     3781 cccaaaccaa taacccgatg caattgacgg gtaaggataa aaaggcgttc tagccgcccc
     3841 agtcccaaaa tatataagcc ccggcgaccg ccggggctct tttttttact gaattgactc
     3901 ctacaacctg aggatggcgc aatccggatg cgcaggattc acaaccaatt cctgcctctg
     3961 catatcgatc aacacgtcca tctcttccat cggaatagca cccaataagg gttcacaatc
     4021 ctctggcaac acaaaagcat tacaggtggc tctccgattg gcaaatctga cctcgatagg
     4081 ccccacgaca tcacagtcca taacccggcc atcggccagc acagctggtt gcttgtactg
     4141 gatgggaaga tccaggtaag cctggatatt ctcgttaata gccatcaaat acgacccact
     4201 gtctaccagc attcgtaccc gtatacttct gacctcgtcc tcgccaatca catgacgacg
     4261 cgccatttgg aaatccgttc cattgatcaa ctctatttca gcatgtactg ctcccataaa
     4321 aactaggatt tttactcaat aacaaaattg ttacttaccc ttttacagta cagcaaggaa
     4381 gcccaagggc acgggaaagg cactaaccca aatataaata ttttcctact ttcactgaat
     4441 gaaaaaactt gtccccatcc tcttcaccct cgcaatcctc atcacctgcg tcactacccg
     4501 caccttcgcc ggccccgtcg acaccatcct cgtccacagc gccgccatga acaaggacat
     4561 caaatgcgtg gtgatcagcc ccggcggcaa acccgccccg ggcgcccact ggcccgtcat
     4621 ctatttatta cacggctata gcggcgacta cgcccaatgg atccacaccg ccccgcagct
     4681 tgcacaggag tctaccgccg atggcatcct cttcgtctgt cccgacggcg gctatgacag
     4741 ctggtacttc gacagccccg tcgacccttc ggtacgctac gagaccttca tcacctccga
     4801 actgatcccc tatatcgacc ggcattactc caccctgccc gataagaccc accgggcgat
     4861 caccggcctc agcatgggcg gacatggcgc cctctacctg gcgatccgcc accgcgacct
     4921 cttcggcgcc gcaggcgcta ccagcggcgg cgtcgacttc cggcccttcc ccgataactg
     4981 gggtataaa
//
LOCUS       DATGFG010000869         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1206355, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000869 DATGFG010000000
VERSION     DATGFG010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206355"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3065
                     /locus_tag="VKQ52_16365"
     CDS             <1..3065
                     /locus_tag="VKQ52_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLZ88831.1"
                     /translation="AYFDGGCPCEHGRPIHACAGGTDNVELAAMTAPRPQLLVSDGHD
                     WTAHMPEHDFPFLQHIYSYFGDTSLVENIHLPNDNHDYGINKRVAMYRFMAGRLGLNL
                     SAIRHRDGSIDESRCTVEPDSALFVFGPHGENLPADAIMGFANLERAWNNRYAAPASL
                     GNSSTGPVNTQPRYKVAVVDLMLLKRQKPGAIPLAKTVGADGLELDMGGLGNRETFEN
                     SLANDSIRRQFLEAAQADSIEFASLAMTGFYSQNFATRPTYQQMIGDCIATMKEMHIH
                     IAFLPLGVNADPGKHPELRPVLVQRLKIAGKMAEQAGVIIGIETTLPAADELQLLKDI
                     GSPAIRSYFNFANALDAHRDLQKELQTLGKDNICQIHCTNTDGVWLQNDSAIDMRKVK
                     ATLDELGWRGWLVVERSRDAKDPHNVKKNFRANTHYVKSIFQPGAVIEVAPNGKSLGA
                     ALREACELRRLADPSVKNGIHIVMKGGEYPLTEPLFLRPEDSGTPTSPTIIEAAPGET
                     PVLSGAIAVSNWRHAAGAPTGLPAAATANLWEADIPAMQPFRQLWINGIKATRARDVD
                     DDDQMSRILSIDKKEQAIYIPATRTLPTDPGKMEMVIHQMWAIAILRIRSIEKLNNSY
                     KLTFKEPESHLEFEHPWPAPVLDSAHKQNGSSAYYLTNALEFLDHPGEWYADEQKGKL
                     YYWPRAGEDMTTAQARIPALTQLVITQGTIDKPVHDIQWQGIRFSYAGWLRPSEKGHV
                     PLQAGMYLLDAYKLKIPGTPDKKGLENQAWIGRPSAAVEISYATRLVFRQCIFSHLAS
                     TGLDFIRGTSDDSTTRCLFRDIGGTGLQLGVYSDPAFETHLPYNPTDTREVCHDEWIT
                     DNTVTDCTNEDWGTVGISAGYVHDIHIEHNEVSEVSYSGICVGWGWTRTINCMRNNAI
                     IGNYVHHYAKHMFDVGGIYTLSAQPGTLITGNRIDSIYHPPYAHDPKHWFYFYFDEGS
                     SFITIKDNWCPEEKFMQNSNGPGNTWENNGPTVADAIKLSAGVRNTLK"
     gene            3070..>3341
                     /locus_tag="VKQ52_16370"
     CDS             3070..>3341
                     /locus_tag="VKQ52_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ88832.1"
                     /translation="MLRLGILGLGEGRSTMSAALNSPKWQLKKICDLNEDLCRHRAEE
                     FNFPHYTLSYQNLLDDPEIDVIGIYTPDKFHATHVKMALSHGKHVV"
BASE COUNT          819 a         1152 c          778 g          592 t
ORIGIN      
        1 ccgcatactt cgacggcggc tgcccctgcg aacacggccg tcccatccac gcctgcgccg
       61 gcggcaccga taatgtcgaa ctggccgcca tgaccgcccc ccggccccag ctccttgtct
      121 ccgacggtca cgattggacg gcccatatgc ccgaacacga tttccccttc ctgcaacaca
      181 tctatagtta tttcggcgac accagcctcg tcgagaatat ccacctgcca aatgacaacc
      241 acgactatgg gatcaacaaa cgcgtcgcca tgtaccgctt catggccggg cggctcggcc
      301 tcaacctgag cgccatccgg cacagggacg gcagcatcga cgagagtcgc tgcaccgtgg
      361 aacccgattc cgccctcttt gtctttggcc cccacggcga gaacctcccg gccgacgcca
      421 tcatgggctt tgccaacctc gaacgggcct ggaacaaccg ctatgcggca cccgcctccc
      481 tcggcaattc atccaccggc ccggtcaaca cccagccgcg atataaggtc gccgttgtcg
      541 atctcatgct cctcaaacgc cagaaacccg gtgccatccc cctggcaaag accgtcggcg
      601 ccgatggcct cgaactcgac atggggggcc tcggcaaccg cgaaacattt gagaacagcc
      661 tggcgaatga ctccatccgc cgccaattcc tcgaagccgc ccaggccgac agcatcgaat
      721 tcgcctccct cgcgatgacc ggcttctact cccaaaactt cgccacccgg ccaacatacc
      781 agcagatgat cggcgactgc atagccacga tgaaagagat gcacattcac atcgccttcc
      841 tcccgctcgg tgtcaacgcc gatcccggca aacaccccga actccggccg gtactcgtcc
      901 aacgcctgaa aatagctggt aaaatggcgg aacaggccgg cgtcatcatc ggcatcgaaa
      961 caaccctccc cgccgccgac gagttgcagc tcctcaaaga catcggctcc cccgccataa
     1021 gatcctattt caactttgcc aacgccctcg atgctcaccg cgatctgcaa aaagaactcc
     1081 aaaccctcgg caaagacaat atctgccaga tccattgcac caataccgac ggcgtatggc
     1141 tgcaaaacga tagcgccatc gacatgcgga aagtaaaagc caccctcgat gagctgggct
     1201 ggcggggctg gctcgtcgtc gaacgctccc gcgacgcaaa agacccccat aacgtcaaaa
     1261 agaacttcag ggccaacacc cactatgtca aatccatttt ccaacccggg gccgttatcg
     1321 aggttgcacc aaacggtaag agcctcggcg ccgcattgcg ggaagcctgt gaactccgac
     1381 gtctcgccga cccctcggta aagaacggta tccatatcgt gatgaaaggc ggtgaatacc
     1441 ccctcaccga acccctcttc ctccggcccg aagacagcgg tacgcccaca agccccacga
     1501 tcatcgaagc cgcgcccggc gagacgcctg tcctcagcgg cgctattgcc gttagcaact
     1561 ggcgacatgc cgccggtgcg ccaaccggtc tgcccgctgc cgccaccgcc aacctctggg
     1621 aagccgatat cccggcgatg cagcccttcc gccaactctg gatcaacggc ataaaagcca
     1681 cccgcgcccg cgacgtcgac gatgacgatc aaatgtcccg tatcctctcc atcgacaaaa
     1741 aagaacaggc catttatatt cccgcaaccc ggaccctgcc caccgatccc gggaaaatgg
     1801 aaatggtcat ccaccagatg tgggccatcg ccattctccg tatcagatcc atcgaaaaac
     1861 taaacaacag ctataaactc accttcaaag aacccgaatc ccacctcgaa ttcgaacacc
     1921 cctggcccgc ccccgtcctc gatagcgcac acaaacagaa cggcagctcc gcctactatc
     1981 tcacaaacgc cctcgaattc ctcgaccatc cgggggaatg gtatgccgac gaacaaaaag
     2041 gaaaattgta ttactggccg cgagccgggg aagacatgac cacggcacag gcccgcattc
     2101 ccgcactcac ccaactcgtc ataacacagg gaaccatcga caagccggta cacgatatcc
     2161 aatggcaggg tatccggttc tcctatgccg gctggctccg gccctccgaa aaggggcatg
     2221 ttcctttgca ggcaggtatg tatctcctgg acgcctacaa gctgaagata cccgggacgc
     2281 cggataagaa aggcctcgag aaccaggcct ggatcggccg gccttccgcg gcggtagaga
     2341 tcagctacgc cacccgcctc gtcttccgtc aatgcatctt ttcccatctc gcctccacgg
     2401 gtctcgattt catccgcggg accagcgacg acagtaccac ccgttgcctc ttccgggaca
     2461 tcggtggcac cggcttgcag ctcggcgtat actcagaccc cgctttcgaa acccacctgc
     2521 cctataatcc aaccgacacc cgcgaagtct gccacgacga atggatcacc gataataccg
     2581 tcaccgactg taccaacgag gactggggaa cggtaggcat cagcgccggc tacgtccatg
     2641 atatccatat cgaacacaat gaggtaagcg aagtatccta ttccggaatt tgtgtcggtt
     2701 ggggatggac ccgcacgatc aactgtatgc ggaataacgc catcatcggc aattatgtcc
     2761 accattacgc aaagcatatg ttcgatgtag gaggcatcta taccctctcg gctcagccgg
     2821 gcaccctgat cacgggtaac cgcatcgatt cgatctacca cccgccctat gctcatgacc
     2881 ccaaacactg gttctatttt tattttgacg agggatcgtc ctttatcacg ataaaagaca
     2941 actggtgccc ggaagaaaaa ttcatgcaga actcgaacgg accgggcaat acctgggaaa
     3001 ataatggccc gaccgtagcc gatgccatca aactatcggc cggagtaaga aatactttaa
     3061 aataagacta tgttacgact cggaatcctc ggacttggcg aaggccggag caccatgtct
     3121 gccgccctca acagccccaa atggcaatta aagaagatct gcgatctcaa tgaagacctc
     3181 tgccgccacc gcgcggagga attcaacttc ccgcattata ccctcagcta ccagaacctc
     3241 ctcgatgacc ccgagatcga tgtcatcggc atctataccc ccgataaatt tcacgccacc
     3301 catgtaaaga tggccctgtc ccacggcaaa catgtcgtct g
//
LOCUS       DATGFG010000870         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_389080, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000870 DATGFG010000000
VERSION     DATGFG010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389080"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..612
                     /locus_tag="VKQ52_16375"
     CDS             214..612
                     /locus_tag="VKQ52_16375"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ88833.1"
                     /translation="MAVKRNAEIFENYLAYIDRHLQDIVAGDVDEMLELREIARGLYI
                     HPTHLSNVIKEYTGKHPCYFYEQKILAVAKRLLDDPNQSIASVARRLTYDPSNFTKWF
                     RTYAGISPSEYRRSAPAALVFSTRSCRAAG"
     gene            complement(628..1458)
                     /locus_tag="VKQ52_16380"
     CDS             complement(628..1458)
                     /locus_tag="VKQ52_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88834.1"
                     /translation="MRLFIILLLSLAARVAFAQANDSTASAHFIIGATANSGLNYYGR
                     VDSLHSKALYPFIGVALKNGLYCNANFVFIQNSLQSQYAATIVEGGYNFKDSKGHWAG
                     NVSVSRFFYQQDINLIQSAIKQTASASITRLNKIVDLTLGANVKWSDKTDFGVQAGLD
                     HIFRFPHIFGDDDVIVLDPGVNVYAGTRNYTQTYYEKRDLLIFPIAEQQVTTTSQQFV
                     VLAYEWSIPLVYGYKKFNFILTPAYILPQNLLVVPGQPSLSENGANLFYVTASVKFTL
                     "
     gene            complement(1517..1936)
                     /locus_tag="VKQ52_16385"
     CDS             complement(1517..1936)
                     /locus_tag="VKQ52_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88835.1"
                     /translation="MKRFAITSIILASLAISNHSFAQLRLGAASSTRLTSAASVNTPA
                     INNALRASASTSANAASATAATARSAATTAKTTMATTVDASHQAAAATVTKTEQVKDN
                     TRVNASASTSANAAVAPQKVHADADVKVKATAGTGNH"
     gene            complement(2047..>2482)
                     /locus_tag="VKQ52_16390"
     CDS             complement(2047..>2482)
                     /locus_tag="VKQ52_16390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ88836.1"
                     /translation="TLLLWPADKNALYRRLGDTQMVGLTERGGNTDTLMQEAAGYFNH
                     REFAKALPLLDQAVKADSTNQLALFYRGVASWHTGAIRAARSDLEKVYNGESLLRYEA
                     AFYLALSYADEKNNDAAREWLRRIPAGAPVSARAKELETSLK"
BASE COUNT          527 a          605 c          736 g          614 t
ORIGIN      
        1 gcgtttttac ttccggccga ccggtgtcga gctggtccat atcttttccc aatcgggttc
       61 cgactgtggt tttgcgcccg gtgtgcttac cggggcgttc tttcgaaaga cctcatcgtt
      121 gatcccggtg atccaggatc tttccgggca ccggaccgct atgggtgaat aaggtcctgg
      181 tgtggtatcc cggaattggg gtaactttgc agcatggcag tcaagcgcaa cgcagaaata
      241 ttcgagaatt acctggccta tatcgaccgg cacctgcagg atatcgtggc cggcgatgtg
      301 gatgagatgc tggaactgag ggagatcgct cgcggacttt atatccatcc gacgcacctc
      361 agcaatgtca tcaaggaata cacgggaaag catccctgct atttctatga acagaagatc
      421 cttgccgtcg cgaaacgcct gctggacgat ccgaatcagt ccatcgcgtc tgtggcgcgc
      481 cggctgacct atgatccttc caattttacc aaatggttcc gcacctatgc ggggatcagt
      541 ccatcggaat accgtaggtc ggctccggcg gcccttgtat tctcaacaag atcctgtcgg
      601 gccgcagggt aagtaggata aatcgctcta caaagtaaat tttaccgagg ccgtgacata
      661 gaacaggttg gccccattct cggagaggga gggctggccg ggtacgacaa gcaggttctg
      721 cggtagtata taggcgggtg tgagaatgaa attgaacttc ttatacccat acaccagggg
      781 aatgctccat tcataggcca ggacaacgaa ttgttgactg gtagtggtca cctgctgttc
      841 ggctatgggg aagatgagca gatcccgttt ctcgtaatac gtttgcgtat aattccgggt
      901 gccggcatag acattgacac cggggtccag tacgatgacg tcatcgtcgc caaaaatatg
      961 ggggaagcgg aaaatatgat ccaggccggc ctgaacgccg aagtcggtct tatcgctcca
     1021 tttgacgttg gccccaagcg tgagatcgac gatcttgttc aggcgggtga tgcttgccga
     1081 tgccgtctgt tttatggcgg actggatcag gttgatatcc tgctggtaga agaaccggga
     1141 cacggagacg ttaccggccc agtggccttt gctgtcttta aaattgtaac ctccttcgac
     1201 gatggttgcc gcgtattgtg actgcaggct gttctgaatg aatacaaaat ttgcattgca
     1261 atagaggcca tttttcagtg cgacgcctat aaagggatag agcgctttgc tgtggaggct
     1321 gtccacgcgg ccatagtagt tgaggccgga gttcgccgtc gcaccaatga taaaatgggc
     1381 agatgctgtg gagtcgtttg cctgggcgaa tgcgacccgg gcggcaaggg agagaaggag
     1441 gattataaat aaacgcatga tccggaattg aagatgaaga ctgcggctgc ctttcggcaa
     1501 ccgcagtcat agggatttag tgattgcctg taccggccgt tgcttttacc ttgacatcgg
     1561 cgtccgcatg caccttttga ggagccacgg cggcattggc cgacgtagag gccgaagcat
     1621 ttacccgggt attgtctttt acctgttcgg tttttgtaac ggttgcagcc gcggcctgat
     1681 gggaagcgtc gacggtagtt gccatggtgg tctttgccgt ggtagcggcg gaccttgccg
     1741 tagcggctgt ggcgcttgcg gcattggcgg aagtggacgc ggaagcgcgg agggcattat
     1801 tgatggcggg agtattgaca cttgccgcgg aggtcagccg ggtcgagctt gctgcaccca
     1861 gtctcagttg tgcaaaagaa tgattggaga tggccaggga agcaaggatg atcgaggtga
     1921 ttgcaaaacg tttcataatt cctgatttag tttgttagaa aatgaagggt tgttttgtgc
     1981 cctttacttt atatcaatct aaccaggatc ttgttacccc tattttccgg ggttttttga
     2041 aaaattttat ttcaggctgg tttccaactc cttagcacgt gcggaaacgg gagcgccggc
     2101 cgggatcctg cgcaaccatt cccgggcggc gtcgttattt ttctcatccg cgtaagagag
     2161 cgccaggtag aaggcggctt cgtagcggag cagggattct ccattatata ccttttcgag
     2221 atcgcttcgc gccgccctta tcgcaccggt atgccaggac gcgacgcccc ggtaaaagag
     2281 cgccagttga tttgtgctgt cggcctttac cgcctgatcg agcaggggca gcgcttttgc
     2341 aaactcccta tgattgaaat agccggctgc ttcctgcatc agcgtatcgg tattcccccc
     2401 acgctcggtc agaccgacca tttgcgtatc gccgagccgc cggtagaggg cgttcttgtc
     2461 ggcgggccag agcagcaggg tg
//
LOCUS       DATGFG010000871         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_389297, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000871 DATGFG010000000
VERSION     DATGFG010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389297"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2413
                     /locus_tag="VKQ52_16395"
     CDS             <1..2413
                     /locus_tag="VKQ52_16395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ88837.1"
                     /translation="GSRAAAGVIIITTKKGRSGPLRVRYSGYVGAQNMPKKLPLTNRQ
                     QYQEITTRAEVNAGLPIATANDPSSPSYIGNINTDWQKAAFKTGIIQDHNLNFSGGND
                     ATSYSMSLNYFDQSATIKGGPGEPKYNRYNANASVQSKTGILSFGGRFAFTQSHKQNI
                     TYPHQHPNIGNEIVDLDRAIPTVPVYDSTRLGGFGGTNEVTQKAIMLNIIGMNNLMNS
                     YSNRDRFLGNSWLELEPVKNLKYRLNLVYDRTETRDFFFEPQYDLGWYYLNNTAYMTD
                     SRGAQYTALVENTLSYKFETGKHRLELLAGQTYEVDDIQNMTGLESGFTPPYYYSFGT
                     GNPTTASVTSNEQKATMNSFLGRINYNYGDRYLITFNGRRDGSSKFSPQHRWGNFGSV
                     AAAWNISNEKFIHLPEAVSFLKLRGGYGTLGNQNFPSFYAYTTYINSNASYLFGNTPG
                     SGNQYLAPGSIQTQVADPTLKWETKVTSNLALDLGLLNNTLLFTAEYFINTDKDLIAQ
                     PPIPLSVGATNTPYVNAASLRNSGIEFTVSYKKTFGKFMLNVEANAHTLKNKVLQLGG
                     TNDPIYGAESKTEVGHEVGQIYGYVTEGIFQSTADVHKHAIQPNAAPGDVKFKDIDGN
                     DTINALDQTYLGSAIPKVYYGLNLGLSYGPWDVSVFLQGNAGNKIVNGLYQTLMAGQY
                     TNHYIDELNYWTPTHTNTNIPRPVIGDPNGNDGPSDRWVQSGAYVKLQNAQLGYTLPA
                     AKLNATHVFKSARIFISGQNLLAITRYKGYDPDIISDGLFSRGFDYGSFPNPRTYMAG
                     VQVGF"
     gene            2432..>3442
                     /locus_tag="VKQ52_16400"
     CDS             2432..>3442
                     /locus_tag="VKQ52_16400"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ88838.1"
                     /translation="MKLFFSTILILIILTCCNKQLQQTNPNQETAQVFWRTEADAYNA
                     GNSVYGSLILDGSYMRFTHVILNTRGDDATSNSPWNEIYNCGKFALDVNGFAAQAPFT
                     AYYQGVFRANQVLENVPKITMDTALRDRILGQAYFLRGLYFFHLASIYRNVPIPLTIA
                     QSSAGYYVHQSPQDSVYAQAIADFAHAIPLLPVSYAGLTGPDQGQVGRATKGAAMAFL
                     GKVYLFNKRYADAAQQFLNVINLGVYSLVPNYHDNFTEQDENNQESIFEVQFSRTVGG
                     TADGWGPVDPDATWGKYEARAVTFGPRNFAFGDVQPTKALYYEFLQEKTTGGATDPRL
                     YAT"
BASE COUNT          871 a         1174 c          764 g          633 t
ORIGIN      
        1 tgggtcccgc gccgccgccg gggtgatcat catcaccacg aagaaaggcc gctcaggacc
       61 cctcagggta agatacagcg gctatgtcgg cgcccagaac atgccgaaaa agctaccgct
      121 caccaaccgg caacaatacc aggagatcac cacccgggcc gaagtcaacg ccggcctccc
      181 catcgcgacg gcaaacgacc cttcctctcc gtcctatatc ggcaatatca acaccgactg
      241 gcaaaaggcc gcctttaaga ccggcatcat ccaggaccat aacctgaact tctccggcgg
      301 caacgacgcg acatcctata gtatgtccct caattatttc gaccagagcg ccaccattaa
      361 gggcggaccc ggcgaaccca aatacaaccg ctataatgcc aacgcctccg tccagagcaa
      421 gaccggcatc ctttccttcg gggggcgttt cgcctttacc cagtcacaca aacagaatat
      481 cacctatccc caccaacatc ccaacatcgg caacgagatc gttgatctcg accgggcgat
      541 cccaactgtt ccggtgtatg attccacccg gctcggcggc ttcggcggaa caaatgaagt
      601 aacccaaaaa gcgatcatgc tcaatatcat cgggatgaac aacctgatga acagttacag
      661 caaccgcgat cgcttcctcg gcaactcctg gctcgagctc gaaccggtga aaaatctcaa
      721 ataccggctc aacctcgtct acgaccgcac cgagacccgc gacttcttct tcgaacccca
      781 atacgacctc ggctggtact atctcaacaa caccgcctat atgaccgact cccgcggcgc
      841 ccaatacacg gccctcgtgg agaacacact ctcgtacaaa ttcgagaccg ggaaacaccg
      901 cctcgaactc ctcgccggac aaacctacga agtagacgat atccagaaca tgaccggcct
      961 ggaatccgga ttcaccccgc cctactatta ttcctttggg acagggaacc ctaccacggc
     1021 ctccgtcacc agcaacgaac aaaaggcgac gatgaactcc ttcctcggcc gcatcaatta
     1081 caattacggc gatcgctatc tcatcacctt caacggccgc cgcgacggct cctccaagtt
     1141 cagcccccaa caccgctggg gtaacttcgg ctccgtggca gccgcatgga atatctccaa
     1201 cgaaaagttc atccacctcc ccgaagccgt cagcttcctc aaactccgcg gcggatacgg
     1261 aacactgggc aaccagaact tccccagctt ctatgcctat acaacctata ttaacagcaa
     1321 tgcctcctat ctttttggga atactcccgg cagcggtaac caatacctcg cacccggctc
     1381 tatccagacc caggtggcgg accccacatt aaaatgggaa acaaaggtca cctcgaacct
     1441 cgccctcgat ctcggcctgc tgaacaacac cctcctgttc accgccgaat atttcatcaa
     1501 caccgataaa gacctgatcg cccaaccgcc gatcccccta tccgtaggtg cgacaaacac
     1561 cccgtacgtg aatgcggcat cactccgaaa cagcggcatc gaattcaccg tcagctataa
     1621 aaagaccttc ggtaaattca tgctcaacgt cgaagcaaac gcccatacac taaagaacaa
     1681 agtgttgcag ctcggcggta cgaacgaccc catctatggc gctgaaagca agaccgaagt
     1741 aggccacgag gtcggccaga tctatggcta tgtcaccgaa ggcatcttcc agagcaccgc
     1801 cgacgtccat aaacacgcca tacagccaaa cgcggcgccc ggtgatgtaa aattcaaaga
     1861 catcgacggc aacgatacga tcaatgccct ggatcagacc tacctcggct cggccatccc
     1921 aaaagtctac tatggcctga acctcggtct cagctacggg ccatgggacg tctccgtttt
     1981 cctccagggc aacgcgggta ataagatcgt caacggcctc taccagaccc tcatggccgg
     2041 gcaatacacc aatcactata tcgacgaact caattattgg acgcccacgc ataccaatac
     2101 caatatccca cggcccgtca tcggcgaccc caacggaaac gacggccctt ccgatcgctg
     2161 ggtgcaaagc ggcgcctatg tcaagctgca gaatgcgcaa ctcggctata cccttcccgc
     2221 cgcgaagctg aatgcgaccc acgtcttcaa aagcgcccgc atcttcatct cgggtcagaa
     2281 cctgctcgcc atcacaagat acaagggcta tgacccggat atcatcagcg atgggctttt
     2341 cagcaggggt ttcgattatg gttccttccc caaccccagg acatacatgg ccggtgtgca
     2401 ggtaggattc taaatcttta aaaaattcgt tatgaaactg tttttttcaa ctatactgat
     2461 cctcatcata ctcacctgct gtaacaaaca gctgcaacag acaaacccca accaggagac
     2521 ggcacaggtc ttctggcgga cggaggccga tgcctacaac gccggcaact ccgtctatgg
     2581 cagcctcatc ctggatggct cttatatgcg gttcacccat gtcatcctca acacccgggg
     2641 cgatgacgcc accagcaaca gcccctggaa cgagatctac aactgcggga aattcgcgct
     2701 cgacgtaaac ggcttcgcgg cccaggcccc cttcacggcc tattaccagg gagtgttccg
     2761 cgccaaccag gtgctggaga acgtcccgaa aataaccatg gacacggccc tgagagaccg
     2821 catcctcgga caggcttact tcctccgcgg cctgtatttc ttccatctgg cctccatcta
     2881 ccgcaacgtc cccatccccc tcaccatcgc ccaatcctcc gccggctact atgtacacca
     2941 gtcgccacag gatagcgtct acgctcaggc gatagccgac ttcgcacacg ccatcccgct
     3001 gctgcccgta tcctatgcgg ggctcaccgg ccccgaccag ggacaggttg gccgggcgac
     3061 aaagggcgct gcaatggcgt tcctggggaa ggtctatctc tttaataaac gctatgccga
     3121 cgccgcgcaa caattcctga atgtcattaa cctcggcgtc tactctctcg ttcccaatta
     3181 ccacgacaat ttcaccgaac aggacgagaa caaccaggaa tccatcttcg aggttcagtt
     3241 ctcacggacg gtaggcggca ccgccgatgg atggggaccc gtcgatcccg acgccacctg
     3301 gggaaaatac gaggcccgcg ccgttacctt cggtccccgc aactttgcgt tcggtgacgt
     3361 gcaaccgaca aaagccctct attacgaatt cctgcaggaa aagaccaccg gcggcgccac
     3421 cgatccccgg ctctacgcca cc
//
LOCUS       DATGFG010000872         5097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1064945, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000872 DATGFG010000000
VERSION     DATGFG010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064945"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VKQ52_16405"
     CDS             complement(<1..632)
                     /locus_tag="VKQ52_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ88839.1"
                     /translation="MNMPANHPQPVLEVENLDVRYGSFQAVQKVSFQVLPGEIFGLLG
                     PNGAGKTSTLSAIEGLLKPNGGSISVAGFDSRNQPIHARASMGVQLQSTSFQPELTVA
                     EIIQLYAGIHGIALSTEKTNTILADIKLESSATKRFGQLSGGQQQRVSLVIATIHQPP
                     LVLMDEPTTGLDPQSRRQLWEKIEGMREKGHAILLTTHSMEEAESVCDRIA"
     gene            856..1242
                     /locus_tag="VKQ52_16410"
     CDS             856..1242
                     /locus_tag="VKQ52_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88840.1"
                     /translation="MRPFVVALFFLFFAAGINRLQAQSLTNTAWKFYVESLHDTLTMH
                     VGADTSFSTSSSGELIVRSLWKQVKDTIKINDLDGMYPCTAGEGVYRMSIDGDYLSFF
                     MVTDPCNQRADALNGVKFKRTVEMKK"
     gene            complement(1310..1711)
                     /locus_tag="VKQ52_16415"
     CDS             complement(1310..1711)
                     /locus_tag="VKQ52_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88841.1"
                     /translation="MKAVAKIFAVALTAAVVVVSNPLSTFANGGETKKPAEEQVSVKY
                     QGTANNAISFKVEFENPTNEKFSLIIKNDNGDVVYHQQFNEAHFSKNVYFENTDTDIH
                     PTFIIRTANTEIVRQFQVSKIVTENTTVTQL"
     gene            2136..3071
                     /locus_tag="VKQ52_16420"
     CDS             2136..3071
                     /locus_tag="VKQ52_16420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ88842.1"
                     /translation="MIPTYNCSNYLREALLSVLAQDPGAELMQIEVVDDYSTDADVRA
                     LVQELGKGRVGYFRQDRNVGSLRNFETCLNRSTGEWVHLLHGDDLVKPGFYKEIESLF
                     RQFPEAGAAFTGYLHVNERTEVLYPNDQLSDRPCIIKDWLNVIAEGQRVQTPAMVVKR
                     SVYEHLGSFFAFHYGEDWEMWVRIAAHYPVAHSPRHLAKYRVHKKNITSDYFCNGQNI
                     RDIEKAIEIVQEYLPEDKRKVLYERAKRHYSRYFARTSDMVYHSYHHPQQALAQAKSA
                     FQMDMNPTTLYFVCKMYMKLLIRYRFKDMRQPAFS"
     gene            3103..>5097
                     /locus_tag="VKQ52_16425"
     CDS             3103..>5097
                     /locus_tag="VKQ52_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88843.1"
                     /translation="MRRTLLVTALAFVSIFKVMAQEHPLWMRYPAISPDGETILFSYK
                     GDIYSVAAKGGVAVPLTISESYEFAPVWSHDGKWIAFASDRYGNFDVFVMPSSGGEAR
                     RLTYYSGGEIPSSFTADDKRVLFSANRQDLVTDGQFPTSVMTELYSVAVSGGRVSQVL
                     PVPAVNATVSADGERLIYEDLKGYENDWRKHHTSSVTRDIWMYEAKEGKYTRLTSFKG
                     EDKNPVFGADDREYYYLSEQGGSFNVYKSSVTDPATTVALTHFSKHPVRFLTRAKDNT
                     LCFGFDGEIYTQHPGAEPRPVSINVREDGRMNLEKILPVSGGVTEMKLSPNGKEIAFV
                     FRGEVFVSSVEGGITKRITNTPWQERSVSFSPDGRSLLFAAEKNSNWNIYTLSIVRKE
                     EPYFFASTVLKEETVVATPAEEFQPAFSPDGKEVAYLEDRVTLKVINLGSKQSRTVLS
                     ADKNYSYADGDQYYQWSPDGKWFLVQFGLPSRVMAPEVGLVAADGKSEVIDLTLNGYA
                     DFTPKWGMDGKMMIWGSDREGARQQGGSTITGDVYGLFFSKDAFDRFKLSKEELALLK
                     EAEDKKEKEKKEAEEAAKAKGGKKVDKDTAKKDTVKPVKIDWDNLTDRKLRLTTHTSD
                     ATDFLLSRDGEKLYYLSRFEKGYDLWMTEMRTKETKLFAKL"
BASE COUNT         1113 a         1059 c         1650 g         1275 t
ORIGIN      
        1 gcgatgcggt cgcagacgga ttctgcttct tccatcgagt gggttgtcag gaggatggcg
       61 tggccttttt cgcgcatgcc ttcgatcttt tcccagagct gccggcggga ctgggggtcg
      121 aggccggtgg tgggttcatc catcagcacc agtggaggct ggtggatggt ggcgatgacg
      181 agagatacgc gttgctgctg gccgccggat agttgtccga agcgttttgt ggccgagctt
      241 tccaatttta tatcggcgag gatggtgttg gtcttttcgg tggagagggc gatgccgtga
      301 atgccggcgt atagttgaat gatctccgct acggtgagtt cgggttggaa gctggtggac
      361 tgcagctgga cgcccatgga ggcgcgggcg tggatgggtt gattgcggct gtcgaagccg
      421 gccacgctga tggagcctcc gtttggtttt agcagtcctt cgatggcgct gagggtgctt
      481 gtcttccctg cgccattggg tccgaggagg ccgaagatct cgcccggcag cacctggaag
      541 gagacttttt gtactgcctg gaagctgccg tagcggacgt ccaggttctc tacttcgagg
      601 acgggttggg gatggtttgc tggcatgttc atgttactaa gataggatgc cggcggcggc
      661 gggacaagag cagggcggtc attttggggg gaattgccgg tgaagtgccg ggaaagggcg
      721 gcgaaggagc gagttgtggc gatatgccgg tgggggcggc tgcgaatggg ggcaggttac
      781 cttatcgcta agctttttaa ccggcgggag aaaagaaggg ggaacggcta atttgcggaa
      841 ctaaaatttt cttttatgcg tccatttgta gtcgccctgt tctttctttt ttttgccgcg
      901 gggattaacc gcctgcaggc tcagtcgctg accaacactg cctggaaatt ctatgtcgaa
      961 tctttgcacg ataccctgac catgcatgtc ggggccgata ccagtttttc gacctcgagt
     1021 tcgggggaac tgatcgtccg gtcgctttgg aagcaggtga aggatacgat taagatcaat
     1081 gacctggatg ggatgtaccc ctgtacggcg ggggaagggg tctaccgcat gagtatcgac
     1141 ggggattacc tgagcttttt tatggtgacc gatccctgta atcagcgggc cgatgcgttg
     1201 aatggggtga agttcaagcg aacggtggag atgaagaaat agagatgggg aagttatttg
     1261 aaataaaaaa agccgggtcg agcccggctt tcttattgtg gtgagatgat tacaattggg
     1321 taacggtggt gttctcggtt acgatcttgc taacctggaa ctggcgtacg atctcggtat
     1381 tagccgtacg gatgatgaag gtcgggtgga tatcggtatc cgtgttctcg aagtatacgt
     1441 tcttggagaa gtgagcttca ttgaactgct ggtggtaaac tacgtcaccg ttgtcattct
     1501 tgatgatcag ggagaacttc tcgttcgtgg ggttttcgaa ttcaaccttg aaggagatgg
     1561 cgttgttggc agtgccctgg tattttacgg acacctgttc ttctgccggc ttcttcgtct
     1621 caccgccgtt tgcgaaggtg gacagaggat tgcttactac aactacggcc gcggttaatg
     1681 ctactgcgaa aatctttgct acagctttca tactattact attttatatt ttacaaaatt
     1741 gttaactaaa gcgtttgaag gcaggcagtg tcgtcgagga gtatggcgga agaacaatcg
     1801 gagagtaaga tatggcgagc gccgttttgc tgaattgtta cacaaatgta gaacgtatcg
     1861 ggccccggat caaaccgtat ttagtgtcca tccattatgc caaccggggg ttgaatgcta
     1921 ctaaaatatt gatttttaac tatttgatgc gactcttcga atgataacca accctttttg
     1981 aagactgaaa aaaggttggt atcttgtacc tgattgtata gccatacctt gaacttttcc
     2041 ttatcagttc ccctatgagc attgagcgta tacctttagt cccccccact attgccccgg
     2101 tgaattccgg ggtgaggaga cctttgttct cagtgatgat accgacgtac aactgcagca
     2161 attatctgcg ggaggcgttg ctgagtgtgc ttgcacagga tcccggtgct gagctgatgc
     2221 agatcgaggt agtggatgat tatagtaccg atgcggatgt ccgggcgctg gtgcaggagc
     2281 tcggtaaggg aagggtgggt tatttccgcc aggacaggaa tgtggggagt ctgcggaatt
     2341 ttgaaacctg tttgaatcgc agtaccgggg agtgggttca tcttttacac ggggatgatc
     2401 ttgtcaaacc gggtttttat aaggagatcg agtcgttgtt ccggcagttt ccggaggccg
     2461 gggcggcttt tacggggtat ttacatgtca atgaacggac ggaggtgctt tatcccaacg
     2521 atcagttatc ggacaggccc tgtatcatca aggactggct gaatgtgata gcggaggggc
     2581 agcgggtgca aacgcccgcg atggttgtga agcgtagcgt ctatgagcat ttgggaagtt
     2641 tttttgcttt tcactatggg gaggattggg agatgtgggt gcggatcgcg gcgcattacc
     2701 cggtggcgca ttctccccgg cacctggcga aatacagggt tcacaagaag aacatcacca
     2761 gcgattattt ttgcaacggg cagaatatcc gggatatcga gaaggcgata gagatcgtgc
     2821 aggagtatct gccggaggat aagcggaagg ttttgtacga gcgggcgaag cggcattatt
     2881 cgcggtattt tgcccggacg tcggatatgg tctatcacag ttaccatcat cctcaacagg
     2941 cccttgcgca ggcgaagagc gcttttcaaa tggacatgaa tcctaccacc ttatactttg
     3001 tttgtaagat gtatatgaaa ctgctgatcc gttaccgctt caaggacatg cggcagcccg
     3061 ccttttcgta gtagtattat ttcacattat agtctttttt gtatgcgaag aaccttgctg
     3121 gttactgcac tggcctttgt ttctattttc aaggtcatgg cgcaggagca tccgctttgg
     3181 atgcggtatc ctgctatttc cccggatggg gaaacgatcc tttttagtta taagggagat
     3241 atctattccg tcgccgccaa agggggtgtt gcggtgccgc tgacgatcag cgagtcgtat
     3301 gaattcgccc cggtgtggtc gcatgacggg aagtggattg cctttgcttc ggaccggtat
     3361 ggcaatttcg atgtatttgt aatgccttcc tcgggagggg aggccaggag gctgacgtat
     3421 tattcgggtg gggagattcc cagcagcttt accgccgatg acaagcgggt attgttttcc
     3481 gccaaccggc aggacctggt tacggacggg cagttcccta cttccgttat gacggaacta
     3541 tacagtgtcg ccgttagtgg cggccgggta tcgcaggtgt taccggtgcc ggcggtgaat
     3601 gcgacggtga gcgcggacgg ggagcggctg atctatgagg acctgaaggg atatgagaat
     3661 gactggcgca aacatcacac ctcttcggtg acgagggata tctggatgta tgaggcgaag
     3721 gagggtaaat atacccggtt gacaagtttt aagggggagg acaagaaccc tgtatttggc
     3781 gccgatgaca gggagtatta ttacctgagc gagcaaggcg ggagttttaa cgtatataag
     3841 agttcggtca ccgaccctgc gacgaccgtg gcgctgacgc atttcagcaa gcacccggtc
     3901 cggtttctta cgcgggcgaa ggataatacc ctttgttttg gatttgacgg ggagatctat
     3961 acccagcatc cgggtgcgga accccggccg gtgagtatca atgtccggga agacggccgg
     4021 atgaacctgg agaagatact gccggtgagc ggaggggtga cggaaatgaa gctgtcgccc
     4081 aatgggaagg agatcgcttt tgtctttcgt ggggaggtgt ttgtctcatc ggtggagggt
     4141 gggatcacga agcggatcac gaatacgcca tggcaggagc gaagcgtcag ttttagcccc
     4201 gatgggcgca gcctgttgtt tgcggcggag aagaacagca actggaatat ctatactctt
     4261 tccatcgtgc ggaaggagga accttatttt tttgcttcga cggtgttgaa ggaggagacg
     4321 gtggtggcga cgcctgccga ggaattccag ccggctttct caccggatgg caaggaggtg
     4381 gcgtacctgg aggaccgggt gacgctgaag gtgatcaacc tgggcagcaa acaaagccgg
     4441 acggtgttgt ctgcggacaa gaattactcg tatgccgacg gggatcaata ttatcagtgg
     4501 tcgccggatg ggaagtggtt tcttgtacag ttcgggttac cgagccgggt gatggcgccg
     4561 gaggtagggc ttgtagcggc ggatgggaag tcggaggtga tcgatctcac gctgaacgga
     4621 tatgctgatt ttacgcccaa gtggggcatg gatgggaaga tgatgatctg ggggtcggac
     4681 cgggaagggg cgcggcagca gggtgggagt acgatcacgg gagatgtcta tggattgttc
     4741 ttttcgaagg atgccttcga ccggttcaag ttgtcgaagg aggaattggc cttattgaag
     4801 gaggctgagg ataagaagga gaaagaaaag aaggaggcgg aggaagccgc gaaggcaaag
     4861 ggcgggaaga aggtcgataa ggatacggcg aagaaggata cggtgaagcc ggtgaagatc
     4921 gactgggata acctgaccga ccggaagctg cgcctgacga cgcatacctc cgatgccacg
     4981 gacttcctgt tgtccaggga tggggagaag ctgtattatc tcagccggtt cgagaagggg
     5041 tatgatcttt ggatgacgga gatgcggaca aaggaaacga agctttttgc caaattg
//
LOCUS       DATGFG010000873         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_400315, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000873 DATGFG010000000
VERSION     DATGFG010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400315"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..784)
                     /locus_tag="VKQ52_16430"
     CDS             complement(62..784)
                     /locus_tag="VKQ52_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88844.1"
                     /translation="MEATETKSAKEIVQAAWNKRPLIISGPCSAETESQTVETITRLA
                     KTGKVDIVRAGIWKPRTRPGSFEGVGTKGLPWLQQARKLTGLPVAVEVATAKQVEDAL
                     HFDVDVLWIGARTTVNPFSVQEVADALRGADVPVLIKNPINPDIELWTGAVERVAKAG
                     IKQIGLIHRGFSSYGNTEYRNAPMWHLAIEMKRRTPGLMMINDPSHISGRRDILLDVA
                     QKAIDLDMDGLIIETHIDPDNA"
     gene            complement(840..2504)
                     /locus_tag="VKQ52_16435"
     CDS             complement(840..2504)
                     /locus_tag="VKQ52_16435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLZ88845.1"
                     /translation="MTTFEGLGLDSRLVQAIDELGFKQPTPIQEQAIPVLLSGTKDLV
                     GLAQTGTGKTAAFGLPLLQLVDAGLKYPQALVVCPTRELCMQIVNEVELFKKFIPGMN
                     ALAVYGGTSIGLQIRDLRRGVQIVVATPGRLIDLIERKAINLEQISYVVLDEADEMLN
                     MGFQDDIEFILQNTPKRDATWLFSATMPPEIRRVSKKYMQSPLEITVGKMNTANKNID
                     HQYYVASAHHRYETLKRIIDFNPGIYGIIFTRTKVDAQSIAEKLTREGYDIDALHGDL
                     TQQQRDKVMGEFRERTLQLLIATDVAARGIDVKDITHVINYELPDDVEVYTHRSGRTG
                     RAGSTGIALSIVHSREIGKLRQIERIVQVPFHKMEIPSGKDVCRKQFFSFMDKLLQAD
                     ISHGDYETYVPMLAEKFADISKEEVLKRVAAMEFDRFLKYYENAEDLNVRERSMGRDR
                     DVRGGEKREYGRREDVRGKGREQNGDYTKLFVNLGTKDGFYKASFLQFILDMSDLRKE
                     VLGRIDMKEMNSWIEVEKRSAAQMIRAIDGKNYKGRRIRMNDANSR"
BASE COUNT          530 a          883 c          560 g          658 t
ORIGIN      
        1 catttgatgc tgtccagcag ctcggccagc cgctcgggcg tgatctgctg cttggcatcg
       61 ctcacgcatt gtccggatcg atatgggttt cgatgatcaa tccatccatg tccaggtcga
      121 tcgccttctg cgccacatcc agcaggatat cccgccgtcc gctgatatgc gaagggtcat
      181 tgatcatcat caggcccggc gtgcgccgct tcatttcgat cgccagatgc cacatcgggg
      241 cattccggta ttcggtgttg ccataagagc tgaacccgcg atggatcagg ccgatctgtt
      301 tgatcccggc cttggccact cgctcgacgg ccccggtcca taattcgata tcaggattga
      361 tcgggttctt gatcagcacc ggcacatccg cgccacgaag ggcatcggcc acctcctgca
      421 cactgaacgg gttcaccgtt gtccgggcgc cgatccaaag cacatctaca tcgaaatgca
      481 aggcatcctc tacttgtttg gcggtagcaa cctccacagc cacgggcagc cccgtcagct
      541 tccttgcctg ttgcaaccag ggcaacccct tcgtcccgac gccttcaaaa ctacccggac
      601 gggtacgcgg cttccagata ccggccctta caatgtccac cttacccgtc tttgccagcc
      661 gggttatcgt ctctacggtc tgggattccg tttccgcact gcagggaccg gaaatgatca
      721 gcgggcgctt gttccaggct gcctggacaa tttccttcgc gctctttgtt tctgttgctt
      781 ccatattagt aacttttaat agtactcttt ttaacatcgc aggtcactga cccggcggat
      841 caacggctat tcgcatcatt catccggatc ctgcggccct tgtaattctt gccgtcgatg
      901 gcccggatca tctgcgcggc cgaccgcttt tcaacctcga tccaactgtt catctccttc
      961 atatcgatcc gcccaagcac ctccttccgc agatcgctca tatcgaggat gaactgcaaa
     1021 aaactcgcct tatagaaccc atccttcgtc cccaggttca caaacagctt cgtataatcg
     1081 ccattttgct ccctgccctt tccacgaaca tcctccctgc gcccatattc ccttttctct
     1141 ccaccccgga catccctgtc ccggcccatg ctccgttccc ttacattcag gtcctccgca
     1201 ttctcatagt atttcagaaa ccggtcgaac tccatcgccg ccacccgctt caacacctct
     1261 tccttgctga tatccgcgaa tttctcggcc agcatcggaa cataggtctc ataatcccca
     1321 tggctgatat cggcctgtaa taacttatcc ataaaagaaa agaactgctt ccggcacaca
     1381 tccttcccgc tcgggatctc catcttgtga aacggcacct gcacgatccg ctctatctgc
     1441 cgcagcttcc cgatctcccg gctgtgcacg atcgacagcg cgatcccggt acttccggcc
     1501 cttcccgtac gcccgcttcg atgggtatac acctcgacgt catcgggtag ttcataattg
     1561 atcacatgcg tgatgtcctt tacatcgatc cccctcgccg ccacatccgt tgcgatcagc
     1621 aattgcagcg tccgctccct gaactccccc atcaccttgt cccgctgctg ctgcgtaaga
     1681 tccccatgca gcgcatcaat atcataccct tccctcgtca gcttctcggc aatactctgc
     1741 gcatccacct tggtacgggt aaaaatgatg ccatagatcc cgggattgaa atcgatgatc
     1801 cgcttcagcg tctcataccg gtgatgggcc gaggccacat aatactggtg atcgatgttc
     1861 ttattcgccg tattcatctt gcccaccgtt atctccagcg gcgactgcat atatttcttg
     1921 ctcacccgcc ggatctcggg cggcatcgtg gcgctgaaca gccaggtcgc atcccgcttg
     1981 ggcgtattct gcaaaatgaa ctcgatatcg tcctggaaac ccatattcaa catctcatcc
     2041 gcctcgtcca gcaccacata gcttatctgc tccaggttga tcgccttgcg ttcgataagg
     2101 tcgatcagcc ggcccggggt agccaccacg atctgtactc ccctgcgcag gtcccggatc
     2161 tgcaaaccga tggaagtccc gccatacacc gccagcgcat tcatcccggg gatgaacttc
     2221 ttaaaaagct ccacctcatt tacgatctgc atacataatt cccgggtcgg acataccacc
     2281 agcgcctgcg gatatttcaa ccccgcatcc accagttgca acagcggcag gccaaatgcc
     2341 gccgtcttcc ccgtacccgt ctgtgccagt cctaccaggt ccttcgttcc gcttaataat
     2401 acaggaatgg cctgttcctg tatgggtgtc ggctgcttga accccaactc gtcaatggcc
     2461 tgtacaagcc gggaatccag acctaaccct tcaaatgtcg tcatatgagc ttaaaatttg
     2521 ccgcaaaggt acgcattttg ctccacctca ccttacttaa tgatcctctt gatcttattg
     2581 gcattctcca tcaattccct caaataatcc gcattttcct tctccaggca g
//
LOCUS       DATGFG010000874         4020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_389441, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000874 DATGFG010000000
VERSION     DATGFG010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389441"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VKQ52_16440"
     CDS             complement(<1..1310)
                     /locus_tag="VKQ52_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88846.1"
                     /translation="MYDVAPGLPTLVSGQPSVVTSISSNLHHYCLLDDQGNVYCWGDN
                     AYGEIGIGTTGGSVGTPSKITVDSLGNPFTNIVQTVAGGGAAGYASAALKSDGTVWVW
                     GATSGGNRGNGTWGGTTTRPVQVPFPAGTVIKKILFYMIGMALDANGNVWTWGGNSGW
                     SVPFMLGQGTATPDVTTPHIISLPSAAKDIVGGSLTNYALLANGQLWGWGYYGSYLGL
                     GATASTGLSPVLMNSVLGFSQAITSIGVNNESAYVILADSTLWSWGDNVCGSIGNGLE
                     PNYATYVNSSGVASPYAWDWGANEDIVPKPVQIAQGLHSFTNIWTTVSDVFYCYAEDV
                     NGQLYSWGRNKYGVLGNGVMSASGDIGAIYPNSWDVPYITAINPFSLTKISSTSSPYC
                     ILNPSGSPCNEYAIPSVAAPTANAGPNQNVTTSTTTLNGSASTA"
     gene            1852..3213
                     /locus_tag="VKQ52_16445"
     CDS             1852..3213
                     /locus_tag="VKQ52_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HLZ88847.1"
                     /translation="MKKYFSSYWIRSAFFSILQRFSVTLFGLINFIVLIRWSLSKEQM
                     GTWSLFLAITTIFETTKSGLLKNAHIRYVSTTDETAEKTIIASSSLLINISISIVFIL
                     LIIFFSGNLSSWLHAGSDLGTTLKWFIPGLICMVFFSHMEAIQQSHLDFKGVFAGYFV
                     RQIIFFALIMGTALLHIPFSLPWLALYQSASIAIGTIVISLYSRKYIIGRFDPSRVWI
                     KKIFGYGGYIFGSGLLGNIFASVDQFMVASFKLSNTWVAYYSAASKLNQVVDIPSYAA
                     AEVIFPKVSQASINEGREKVRYLYEKMVAILLAFTIPTAIFVILFPKFVIILIAGKNY
                     MAAAPILQMYMITGLLRPMQNQAANLLNSIGKPRLCFIINGISLGVNLVINYICISKI
                     GNYGSAVGTMITCLLGATCWYFIMRRQIGAILPTMARYIADYYKIFFTYAKGFLAPQP
                     SGK"
     gene            3227..>4020
                     /locus_tag="VKQ52_16450"
     CDS             3227..>4020
                     /locus_tag="VKQ52_16450"
                     /inference="COORDINATES: protein motif:HMM:NF014536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HLZ88848.1"
                     /translation="MRIAHLILTHKNPQQLERLIRVLDHPAFDFYIHVDKKVDQTPFN
                     FLAQKKNVFFIENRVRIYWAGYGTIQATLNGFQQILPKNYDYINVTSGQDFPLHSPDH
                     IYQYLAQRKGLEFITCESIEDEWKDAAPRVKQYHLINWRIPGKHRLEILTNKMLEKKL
                     FPSRKFPLDYKIVGRSNWFTLTTPAVQYIVDFLQQHPEVVRYFKFCWGADEFIFASVL
                     YNSAFKDRIRDSLVYVDWTGKKDGHPRILDVNDFEKMKASGKLFGR"
BASE COUNT         1059 a         1203 c          818 g          940 t
ORIGIN      
        1 ttcgccgtcg atgccgaccc attcagcgtc gtcgtcgacg tagtaacatt ctggttgggc
       61 ccggcattgg cagtcggcgc agccacactc gggatggcat attcattaca gggcgagccc
      121 gaaggattca gtatgcaata gggagaggaa gtagacgata ttttcgtgag actgaaagga
      181 ttgatcgccg taatatacgg tacgtcccag gagttcggat aaatggcccc gatatccccg
      241 gatgccgaca tcaccccatt acccagaacc ccatacttgt tgcgccccca actgtataac
      301 tggccgttca catcctcggc atagcaatag aaaacatcac tcacagtcgt ccatatattc
      361 gtaaaactat gcaagccctg cgctatctgc acaggcttgg gaacaatatc ttcatttgca
      421 ccccagtccc aggcataggg cgaagcaacc ccggaactat tcacataggt cgcataattg
      481 ggctccagtc cgttcccgat gctgccgcat acattgtccc cccaggacca caacgtgctg
      541 tcggcgagaa tgacatacgc actttcatta ttcaccccaa tactggtgat cgcctgggaa
      601 aatcccaata ccgagttcat cagtacagga gaaagacccg ttgacgccgt cgcaccaagc
      661 ccgagataac tcccataata cccccatccc cacaactgcc cgttggccag caacgcataa
      721 ttggtaagac tgcccccgac gatgtccttg gctgcactgg gcagggagat tatatgcggc
      781 gtcgtaacat ccggagtggc cgtaccctgg cccagcataa agggaacgct ccaaccggag
      841 ttgccccccc aggtccagac attaccattg gcatcgagcg ccataccgat catatagaac
      901 agtatcttct tgatcaccgt tcccgccgga aaaggcacct gcacgggccg ggttgtagtc
      961 cccccccagg ttccattccc ccggttacca cccgacgtag caccccatac ccaaacggtc
     1021 ccatcactct tcaatgccgc cgaagcatac cccgcagcac cgccgccggc caccgtctgc
     1081 acaatattgg taaaaggatt acccaaacta tctaccgtaa tcttcgacgg agtcccgacc
     1141 gaaccgccgg tcgtcccgat cccgatctcc ccataggcat tgtcccccca gcagtacaca
     1201 ttgccctgat catccagcag gcaataatgg tgcaggttcg aactgatcga agtcaccacc
     1261 gaaggctgac ccgacaccag ggtcggaagc cccggggcta catcgtacaa cttatgtgtc
     1321 gccgtgggaa gtatgtaaca attgtactct cccggcccaa caatatactt gggcgtctgg
     1381 gaatatgaat aaagggaggt gaaaaagaga agcgtagcta gtagggtcga aattaaatgt
     1441 aaatttctgg ctttcatgat ttggatttta gcatcaataa acacaagtga tcgttctttc
     1501 agtttagctt tgatcaaact agattaccac ttcaaatagg ataggcagaa aggttgcctt
     1561 aaagaatacg atcagaaaac tggctcttaa tagtcttgtc tcagtcgaca atattcatgc
     1621 caaaacgaat atgtttatcg gaatattcct tatcttggaa cagtttccca attctaaata
     1681 atatccttag cattatatag ggaagaaatt ttcaatagtc cgattgacaa taaatattca
     1741 aaaaaacagt tattcgaatc acccctcgga ctacccggaa accagtacga aaaaatcaat
     1801 gttttaacgt aagtttggtg cccgtgctcc aaaaggctta aatttcaacc gttgaaaaaa
     1861 tatttctctt cttactggat ccgttcggct tttttttcca tcctccagcg cttttccgtc
     1921 accctcttcg gcctcataaa tttcatcgta ctcatccgct ggtccctcag taaagaacag
     1981 atgggaacat ggtctctttt cctcgccatc actaccatct tcgaaacgac aaaaagcggc
     2041 ctccttaaga atgcacatat ccgctacgta agtaccaccg acgaaacagc cgaaaaaaca
     2101 atcatcgcct catcttcttt attaataaat atctccatca gcattgtatt catcctgttg
     2161 atcatctttt tctcgggcaa cctcagttcc tggctgcacg caggcagcga tctgggtacc
     2221 accctgaaat ggttcatccc ggggctgatc tgcatggtgt tcttttcaca tatggaagcc
     2281 atccagcagt cccacctcga cttcaaaggc gtattcgcag ggtattttgt ccgccagatc
     2341 atcttctttg ccctcataat gggcaccgcc ctgctccaca tccccttttc cctcccctgg
     2401 ctcgccctct atcaatcagc cagcatcgcc atcggcacga tcgtcatctc cctctatagc
     2461 cggaaataca tcatcggccg cttcgacccc tccagggtct ggataaagaa gatatttggt
     2521 tacggcggct atatcttcgg ctccggcctc ctcggaaata tcttcgccag cgtcgaccag
     2581 ttcatggtcg cttcctttaa actgtcaaac acctgggtgg cctactacag cgccgcctcc
     2641 aaactcaacc aggtagtcga catcccctcc tacgccgccg ccgaggtcat cttcccaaaa
     2701 gtatcccagg cctccatcaa cgaaggcagg gaaaaagtac gctacctcta tgaaaaaatg
     2761 gtggccatcc tgctcgcttt caccatccct accgccatct tcgttatcct gttccccaaa
     2821 ttcgtgatca tcctcatcgc aggaaagaac tacatggcgg cagccccgat cctccagatg
     2881 tacatgatca ccggactcct ccgccctatg cagaaccagg cggccaatct cctgaactcc
     2941 atcggcaaac cccggctatg cttcataatt aacggcattt ccctgggcgt taacctcgta
     3001 attaattata tttgtatatc caagatcggt aactacgggt ccgccgtagg aaccatgatc
     3061 acctgcctgt taggagctac atgctggtat ttcatcatga gaagacagat cggggcaatc
     3121 ctgccaacca tggccaggta catcgccgat tattataaaa tcttttttac gtatgcaaaa
     3181 ggttttctcg caccccaacc atccggtaaa taaaagctga caacggatga gaattgcaca
     3241 tttgatcttg acgcataaga acccgcagca gttggaacgg ctgatcaggg tgctggatca
     3301 tccagccttc gatttctata ttcacgtcga taaaaaagtc gaccagaccc ctttcaactt
     3361 tctcgctcaa aaaaaaaatg tcttcttcat tgagaaccgg gttcgcatct actgggccgg
     3421 ctatggtacc atccaggcca ccctcaatgg cttccaacag attctcccaa aaaattacga
     3481 ttatatcaac gtcacctccg gccaggactt cccactccac tcacccgatc atatctatca
     3541 atacctcgcc cagcgaaaag gcctcgaatt cataacctgc gaatccatcg aagacgaatg
     3601 gaaagacgcc gccccccgcg ttaagcaata tcacctgatc aattggcgca tccccggcaa
     3661 acaccgcctg gagatcctca ccaataaaat gctcgagaaa aaactgttcc cctcccgtaa
     3721 attcccgctg gactacaaga tagtcggccg ttccaactgg ttcaccctca ccacccccgc
     3781 cgtccagtac attgtcgact tcctccaaca acacccggag gtcgtacgct acttcaaatt
     3841 ctgctgggga gccgatgaat tcatcttcgc cagcgtcctc tataactccg ccttcaaaga
     3901 ccggatcagg gacagcctgg tctacgtcga ctggacaggc aaaaaggacg gccatccccg
     3961 catcctcgac gtcaacgact tcgaaaaaat gaaagcctca gggaaactgt tcggaaggaa
//
LOCUS       DATGFG010000875         3715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1645169, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000875 DATGFG010000000
VERSION     DATGFG010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645169"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VKQ52_16455"
     CDS             <1..1765
                     /locus_tag="VKQ52_16455"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLZ88849.1"
                     /translation="ASKQWNAPLEECIAENGAIIHKPTNKKLAYGELAAAASTLEVPA
                     NPTLKDPKDFKILGKSMPRLDIPLKVTGRAVYGIDMELPGMVYASVERCPVFGSKLVS
                     YDDSATLKVKGVIKTIKAERVLGKNRYEGVAVIAENYWAALKGRKALKVQWDHQGNDT
                     FTTTAYEQHLRDLAATDGLVAHDSGDFDKAMAGADDKHKLEALYETPVVAHATMEPMS
                     CLAHWQEGDKVDIWTSVQGAGIVKRELAETLGIPQDNITAHISFLGGGFGRRLAADYA
                     GEAAGLSKNMGKPVKVVWTREDDIQLGPFRPMTFSAMKGAVSAEGKALAFQHKVISPS
                     INATKSDKYDKTKPDRSMTEGISDQDYEFPNMKNAYVFADIHIPMQPWRSVTSSTLAF
                     AHECFIDEMAAAAGKDPLQFRLDLLTKDSDTKRVLTKLKEVSHWDHPLPKGRGRGIAQ
                     YKFFAGLCGNVIEVSTRKDGGIKIEKVISVIDLGTVVNPDMVKAQVEGAIVMGIVAAT
                     KDGVIFEGGKSVQSNFHNNRMLRINEMPAVEVHILAEGGPVIKGVGEPGLPPVAPALA
                     NAVFAATGKRIRKLPFDLNKV"
     gene            1772..3340
                     /locus_tag="VKQ52_16460"
     CDS             1772..3340
                     /locus_tag="VKQ52_16460"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLZ88850.1"
                     /translation="MNRLLTTALALLLCARLLAQPYNPAIDVQHYDFSLQLTDSNNII
                     HGEAAITLRFRENSPGFTLDLARKNDEGKGMTVTAIREGNKPIRFTQNSEQLIIHSAG
                     APGAPHTYTIRYEGIPADGLIISNNKFGRRGFFGDNWPNRAHNWLPCVDHPSDKATVS
                     FNITAPDHYTVVANGSLKGEQPLPGHRKLTHWVESEPLSPKIMVIGVADFAVDHPGDA
                     MGVPVWNYVYRENKELGFKSYAYALDILPFYITHIGPYPFEKCGNVQSKTRFGGLENA
                     SAIFYYENSVGSPGVESLMAHEIAHQWFGDAATESQWQHLWLSEGFATYMTLCYLENK
                     YGADTLKNSLQQDRLKVISFEKRRLTPIVDTTIKTDYMQLLNPNSYEKGGWVLHMLRR
                     AIGDEAFWKGISTYYTTWRNKNASSADFEKTMETVSGKDLHDFFHQWLNTPGHPHLTI
                     AWKYDPDKKAVIFDLTQGDHLFTFPLELSLDGAVQHIDIHDKTTHVEIPFAAKPTTIV
                     PDPDTNLLADFEFR"
     gene            complement(3364..>3715)
                     /locus_tag="VKQ52_16465"
     CDS             complement(3364..>3715)
                     /locus_tag="VKQ52_16465"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ88851.1"
                     /translation="RSAYAAAKHALHGFFDCLRAEVSPMNVRVTVLTPGYIQTNISQN
                     ALTKDGSKLGHASENIEKGLPADRAAAKIVRAIRREKFEAYIGKTGPEKMALVLNRLA
                     PGLLMRMAPKFIPK"
BASE COUNT          931 a         1323 c          842 g          619 t
ORIGIN      
        1 cgcctcgaaa cagtggaacg cccccctcga agaatgcatc gcggagaacg gcgccatcat
       61 ccacaaaccg acaaacaaaa aactcgccta cggggaactg gcagcggcag cctccaccct
      121 cgaagtaccg gccaacccca cactaaaaga tccaaaagac ttcaaaatac tgggcaagtc
      181 catgccccgc ctggatatcc cgctaaaggt caccggccgc gccgtctatg gcatcgacat
      241 ggagctgccc ggcatggtat acgcctccgt ggaaagatgc cccgtcttcg gcagcaaact
      301 ggtcagctat gacgacagcg ccaccctcaa agtcaagggg gtgataaaaa cgatcaaggc
      361 cgaacgcgtc ctgggcaaga atcgctacga aggcgtagcc gtcatcgccg aaaactactg
      421 ggccgccctg aaaggccgca aagccctgaa agtacaatgg gaccaccagg gcaacgacac
      481 cttcaccacc accgcctacg aacaacacct ccgagacctc gccgccaccg acggcctcgt
      541 cgcccacgat agcggcgact tcgacaaagc catggccgga gccgacgaca aacacaaact
      601 cgaagccctc tacgaaaccc ccgtcgtcgc ccacgcgacc atggaaccca tgagctgtct
      661 cgcccactgg caggaagggg ataaagtaga tatctggaca tccgtccagg gcgccggcat
      721 cgtaaaacgc gaactcgcgg aaaccctcgg cattccccag gacaatatca cggcccatat
      781 ctccttcctc ggcggcggct ttggccgcag actcgcagcc gactacgccg gcgaagccgc
      841 gggcctctca aaaaacatgg gcaagccggt aaaggtcgtc tggacccgcg aagacgatat
      901 ccagctcggc cccttccgcc ccatgacctt ctccgccatg aaaggcgccg tgtcggctga
      961 aggaaaagcc ctggccttcc agcacaaggt catctccccc tccatcaacg cgacaaagtc
     1021 cgacaaatac gataagacaa aacccgatcg ctccatgacc gaaggcatca gcgaccagga
     1081 ctacgaattc cccaacatga agaacgccta cgtcttcgcg gatatccata tcccgatgca
     1141 accctggcgc tccgtcacca gcagcaccct cgccttcgcc catgaatgtt ttatcgacga
     1201 aatggccgcc gcggcaggca aagacccgct gcaattccgg ctggacctcc tgacaaagga
     1261 ctccgacaca aaacgcgtcc tcacaaaact aaaagaggtc tcccactggg accatcccct
     1321 gccaaaaggc cggggccgcg gtatagccca gtataaattc ttcgccggcc tctgcggcaa
     1381 cgtgatcgaa gtatccaccc gcaaagacgg cggcataaag atcgaaaagg ttatctccgt
     1441 catcgacctg ggcaccgtcg tcaaccccga catggtaaaa gcccaggtgg aaggcgccat
     1501 cgtcatgggc atcgtcgcgg ccacaaagga cggcgtcatc ttcgaaggcg gcaagtccgt
     1561 ccaatccaat tttcataaca atcgcatgct gcgcataaac gaaatgccgg ccgtcgaagt
     1621 acatatcctc gccgaaggcg gccccgtcat caaaggagtg ggggaacccg gcctgccccc
     1681 cgtggcgccc gccctggcaa atgccgtctt cgctgctacg ggaaagcgta tccgtaaact
     1741 tcccttcgat ctgaataaag tataaccaaa aatgaacaga cttctcacga cggccctcgc
     1801 cctcctcctc tgtgcccgac tcctcgccca accctataac cccgccatcg atgtgcagca
     1861 ctatgatttt tccctccaac tcaccgatag caacaacatc atccacggcg aagccgccat
     1921 caccctccgg ttcagggaga acagccccgg cttcaccctc gacctggccc gtaagaacga
     1981 cgaaggaaaa ggtatgaccg tcacggccat cagggaaggc aacaaaccca tccgtttcac
     2041 ccagaacagc gaacagctca tcatccattc cgccggcgcc cccggcgccc cgcataccta
     2101 caccatccgg tatgaaggca tccccgccga cggtctcatc atctccaaca acaaattcgg
     2161 tcgccgcggt ttcttcggcg ataactggcc caaccgcgcc cataactggc tgccctgcgt
     2221 cgaccacccc tccgataagg caaccgtctc tttcaacatc accgcccccg accactatac
     2281 ggtagtagcc aacggatcgc tcaaaggaga acaacccctc cccggccacc ggaaactcac
     2341 ccactgggtc gaatccgaac ctctttcccc aaagatcatg gtcatcggcg tcgccgactt
     2401 cgccgtcgac caccccggcg acgccatggg cgttcccgtc tggaactatg tctacaggga
     2461 gaacaaagaa ctgggattta aaagctatgc ctatgccctc gacatcctcc ctttctatat
     2521 cacccatatc ggtccctacc ccttcgagaa atgcggcaac gtccagtcga agacccgctt
     2581 cggcggactg gagaacgcca gcgccatctt ctattacgag aactcggtag gctcccccgg
     2641 tgtggaatcc ctgatggctc acgagatcgc ccaccaatgg tttggcgacg ccgccaccga
     2701 aagccaatgg caacacctct ggctaagcga aggcttcgcc acctatatga ccctctgcta
     2761 cctggaaaat aaatacggcg ccgacaccct caaaaacagc ctgcaacaag atcgcctgaa
     2821 agtgatcagc ttcgaaaaac gccgcctcac ccccatcgtc gataccacga taaaaactga
     2881 ctacatgcaa ctgctgaatc ccaacagcta tgaaaaaggc ggctgggtcc tccacatgct
     2941 ccgccgcgcc atcggcgacg aagccttctg gaaaggtatc tccacctact acaccacctg
     3001 gcgcaataag aacgcctcca gcgccgattt cgaaaaaaca atggaaacgg tcagcggcaa
     3061 agacctccac gacttcttcc atcaatggct aaacaccccc ggtcatcccc acctcaccat
     3121 cgcctggaaa tacgacccgg ataaaaaggc cgtcatcttc gacctcaccc agggcgacca
     3181 cctcttcacc ttccccctcg agctcagcct cgacggcgcg gtccagcaca tcgacatcca
     3241 cgacaagaca acacatgtcg agatcccctt cgccgcaaaa cctaccacca tcgtccccga
     3301 tcccgacacc aacctcctcg cggacttcga attcagataa cttttaagca taatttccta
     3361 ccgctatttt ggtatgaact tcggagccat ccgcatcaac agccccggcg ccagccggtt
     3421 cagcaccagc gccatcttct ccggccccgt cttcccgata tacgcctcga acttctccct
     3481 cctgatcgcg cgcacgatct tcgcggcagc ccggtccgcg ggcaacccct tttcgatatt
     3541 ctcggaggca tggcccagct tcgacccgtc cttggtcaac gcattctgcg aaatattcgt
     3601 ctggatatag cccggcgtaa gcaccgtaac cctgacattc atcggcgaca cctccgcccg
     3661 cagacagtcg aagaacccgt gcagcgcatg cttcgccgcc gcataggcgc tccgc
//
LOCUS       DATGFG010000876         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_455756, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000876 DATGFG010000000
VERSION     DATGFG010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455756"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VKQ52_16470"
     CDS             complement(<1..183)
                     /locus_tag="VKQ52_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88852.1"
                     /translation="MKILLSLFLLMPAASACAGSTDSIPLPTGQGPLLTPFLAPGYSP
                     DIQFTLTGGGIFSFRTK"
     gene            484..888
                     /locus_tag="VKQ52_16475"
     CDS             484..888
                     /locus_tag="VKQ52_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88853.1"
                     /translation="MAVLRFMKAPRIRLHRRSLVKAAMAFAGLLFFVPQLSSKFYLCA
                     NAPVDPFAVRCISHDGNRPADPSGSSLAHERHPSLSLDKRYTVKHVFALLPVSPGLCE
                     RCADGPETCTVGTPPAVHPPQSSHRLRGPPSL"
     gene            1029..1760
                     /locus_tag="VKQ52_16480"
     CDS             1029..1760
                     /locus_tag="VKQ52_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88854.1"
                     /translation="MRILLIGADGNLATVLAKGLRERLYDVDLAFATPAGECLPDWIR
                     HRLIVVVHRYPDFNACEFCRAVRRQNAAMALLMLAAPAQCDVFGGFSAGVDDFIALPA
                     DTREIIVRVNALARRCSSDEYPGNRIRADDIIIDLDSKTVKRAGRAIDVTASEFKLLE
                     YMIRNKNKVLSKEEIVCGVWGRDVEDKVRKLPVHMNNLRGKLAGGNQSGSAIYTVTRR
                     GYLLADDDRPERKGLPPEKEGGITY"
     gene            1863..2456
                     /locus_tag="VKQ52_16485"
     CDS             1863..2456
                     /locus_tag="VKQ52_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88855.1"
                     /translation="MKKLSVKDIETFRGELFHMILFSMAWCLIGEYVLDFRDYAVGLI
                     VVLIAVVWLALYSIRLYDLEGALGENSEPAFAAGTDVQRRRDRQYALILLFEGIAVLL
                     TWSILLKHGRDNWVIPSFAFIAGLHFFPLARVIRLPSYYYLGAWICLLTVAGYLLFAS
                     GRLEDRDASALIAYGCAAGSVLDGIVIVFRVRRELDA"
     gene            2552..2989
                     /locus_tag="VKQ52_16490"
     CDS             2552..2989
                     /locus_tag="VKQ52_16490"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLZ88856.1"
                     /translation="MDTAHFYEVRLNWETGRKGLVSSPVLDSTIEVATPPEFPKGVAG
                     VWSPEHLLVAAVDSCLMTTFLSIAENSRLEFGQFESQALGKLEKVDGKLLINEILLTP
                     RLTIMREADREKAQRVLEKAGDACLISNSVRARIDFRPEIVVQ"
     gene            3038..4237
                     /locus_tag="VKQ52_16495"
     CDS             3038..4237
                     /locus_tag="VKQ52_16495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLZ88857.1"
                     /translation="MKVIVIGGNFAGFTASIEIKRKLKEKAEVLLIDRNPDFLFVPSL
                     IWVPTGRREVRNISIPRREVLERRGVRFVQTVAEKIDPETRTVHTASGDFEYDQLVIA
                     TGPKVNYDIAPGVREHCCYIGTPDGAMKTRQKLEEFKRHPGPIVIGATQQAGCMGAAY
                     EFLFNIEKWLRENSIRRQVDLYWITPETYLGHFGIDGMPLGESMLKAFMKIFRIHYRT
                     GVGVSEVTADEVVLSTGERIASCFTMLMPPFVGVDLIRNSPELGAEPNGFLEVLPSYR
                     HPKYDNIWVAGLTVNVKPPFTPGAVPFGVPKTGFPSDETGKIVAENIVRVANGRTDLR
                     EKAWGKIPGLCVMDAGRKEVLILSNHLFRPRSFAIMLPNVFYDVMKVLLEKYFLWKMR
                     HGYAQLP"
     gene            4287..4961
                     /locus_tag="VKQ52_16500"
     CDS             4287..4961
                     /locus_tag="VKQ52_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ88858.1"
                     /translation="MKLLVIEDEPSLQASITRYFRQEQFACESVGNYADALQKIDDCE
                     YDCVILDINLPGGSGLQLLQRLREDRKKDGVIIISARDSLDDKISGLDHGADDYLTKP
                     FHLSELNARVKALIRRKYSEGSAGMQVGLLYIDLAARTVSTGGQPVGLSQIEYRLLVF
                     LSANKNRVVSRQAIAEHLHVGPSDQAPGPEFVYSHIKNLKRKLKEKGCLEMIHTVYGF
                     GYKLLS"
BASE COUNT         1124 a         1319 c         1469 g         1095 t
ORIGIN      
        1 tttggtgcgg aaggagaata ttccacctcc cgtgagggtg aattggatat cgggggaata
       61 acctggcgca agaaatggcg tgagcaatgg tccttgcccc gtgggaagcg ggatgctatc
      121 ggtagagcct gcgcatgccg atgccgcggg cattagtaga aacagcgata gcaatatttt
      181 caaggcagaa atttttgagc aggatgtggc ggtcgacccc cgatcaccgc gtagcgacag
      241 gtaatgcata aaggtagaat agaaagcggg agatagacgg accgaaggat tagaatatgg
      301 ttaaaatgcc gacgcttgtg tcgaataagg aggatattaa caaagtttcg gtgattatgt
      361 atataccttt gaattctcac ataagcagtt agttttctca taagcagtta gttttggtta
      421 cggcccctgt tttcacaggg gccaattatt ttgtatattg tgaggtctgc cagaactttc
      481 gacatggcag tacttcgatt tatgaaggca ccacggataa ggcttcaccg gagatcactg
      541 gtaaaggcgg caatggcctt tgccggcctg ttgttctttg ttccccagct ttcatctaaa
      601 ttctacctct gcgcaaacgc acctgtcgat ccatttgccg tccgttgcat ttcacatgac
      661 ggtaaccggc cggcagaccc cagtggcagt tcccttgcgc atgaacggca tccttcctta
      721 agcctcgaca agcggtatac cgtcaagcat gtcttcgcgc tgcttccggt gtcgccgggc
      781 ctgtgcgagc gatgtgccga tggcccggag acctgcactg tcgggacccc gccggctgtc
      841 catcctccgc agtcgagcca ccggctaaga ggtccaccct ccctttagcc tattgtattc
      901 ctggttgtaa ataaactggc gctttttgcc ggatggctgt gttttgactc cccgaagtga
      961 tgttcgtctg cagcggcatt tcgcgtggcc ggccgggcgc ccatttttca gcaagaaaaa
     1021 tcaacagcat gcgaatactt ctcatcggtg cggatgggaa tctggccact gttctcgcaa
     1081 aaggactgcg ggaaaggttg tatgatgtcg atctggcttt tgcaaccccg gctggtgaat
     1141 gcctgcccga ttggatccgg caccggctta tcgtcgtggt ccaccgatac cccgacttca
     1201 atgcctgtga attctgccgg gctgtccgga ggcaaaatgc cgccatggcc ctccttatgc
     1261 tggccgcccc cgcgcagtgc gatgtgttcg gaggtttttc ggcaggcgtc gatgacttta
     1321 tagcccttcc tgccgatacc cgggagatca tcgtccgggt aaatgcgctg gcaagaagat
     1381 gttcgtcgga cgaatacccg ggaaacagga tcagggccga cgatatcatc atcgacctgg
     1441 acagtaaaac ggtaaagcgc gccggaaggg cgatcgacgt gacggcaagc gaattcaagc
     1501 tcctggaata tatgatccga aacaagaaca aggttctttc gaaggaggag atagtctgtg
     1561 gggtctgggg tcgggatgtg gaagacaagg tgaggaaact gccggttcac atgaacaacc
     1621 ttcggggcaa actggccggc ggaaaccagt cgggttccgc gatctatacg gtaacgcgcc
     1681 gtggttacct gctggcagac gatgaccggc cggaaagaaa gggcctccct cctgaaaagg
     1741 aggggggcat aacctattaa taactcttat aggaataaag ataaggaccg gatcacattc
     1801 cggaacgaag gcgggcaaga ataacaaagc ccgattttga attaaaaaaa cagtatcgga
     1861 aaatgaaaaa attaagcgta aaagatatcg agacgttcag gggtgagctc ttccatatga
     1921 tcctgttctc gatggcctgg tgcctcatcg gcgaatacgt cctcgacttc cgcgattatg
     1981 ccgtcgggct catcgttgtt cttatcgccg tggtgtggct ggcattgtat tccatcaggc
     2041 tgtatgacct ggaaggcgcg ctgggagaaa atagcgagcc tgcctttgcc gcggggaccg
     2101 atgtccagcg gaggcgggac cgtcaatacg cgctgatcct gcttttcgag ggtatcgcgg
     2161 ttctgctcac gtggtcgatc cttctgaagc atggccgcga caattgggtg ataccctcct
     2221 ttgcgtttat cgcgggactg cacttcttcc cgctggcaag ggtgatccgg ctgccatcct
     2281 actattatct gggcgcctgg atctgtctgc tgacggtggc cggttacctg ctgttcgcct
     2341 cgggaaggct ggaagaccgt gatgccagtg cgctgattgc ctatgggtgt gccgcgggat
     2401 cggtactgga cggcatcgtc atcgttttcc gcgtgcggag ggaactcgac gcgtagctgc
     2461 cggcgcctga tctctgtcat cccgggccgt gaccattgtc acaggcggcc ggccgatgat
     2521 ttaccatctt tgtgatagaa aaaatacaaa gatggatacc gcacattttt acgaagtgag
     2581 actgaattgg gagaccggcc gcaaaggcct tgtcagctcc cccgtactgg actctaccat
     2641 cgaggtggcc acgccgccgg aattccccaa gggagtggcc ggggtctggt cgcccgagca
     2701 tctgctggtc gcggccgtgg acagttgcct gatgaccact ttcctgtcga ttgcggagaa
     2761 ttcccggctc gaattcgggc aatttgaaag ccaggcgctg gggaagctgg agaaggtgga
     2821 tggaaagctc ctcatcaatg agatcctgct gactccccgg ctgacgatca tgcgggaggc
     2881 ggacagggaa aaggcgcaga gggtgttgga aaaggccggg gatgcctgtc tcatctccaa
     2941 ttcggtgagg gcaaggatcg atttccggcc ggagatcgtc gtgcaatagt gtaatcaacg
     3001 gcgcgggcca aggcccgcgc atcataaaac gatcattatg aaagtcatcg ttatcggcgg
     3061 gaattttgcc gggttcaccg cctccataga gataaaaagg aagctgaagg aaaaggccga
     3121 ggtccttctc atcgacagga accccgactt cctgttcgtg ccctcgctta tctgggtacc
     3181 gacagggaga agggaggtga ggaacatcag cattccccgc cgggaggtgc tcgaacgccg
     3241 gggggtgcgc tttgttcaga cagtcgcgga gaagatcgac ccggaaaccc gcaccgtcca
     3301 tactgcttcg ggggatttcg agtatgacca gctggtcatc gccaccggcc ccaaggtcaa
     3361 ctacgatatc gcgcccggcg tccgggagca ttgctgctat atcggcaccc ccgatggcgc
     3421 gatgaagacc aggcagaagc tcgaagaatt caaacgccat ccgggcccca tcgtcatcgg
     3481 ggccacgcag caggccggtt gcatgggcgc cgcctatgaa ttcctcttta atatcgaaaa
     3541 atggctgcgg gaaaacagca tccgcagaca ggtggacctg tactggatca ccccggagac
     3601 ctatctgggc cacttcggta tcgatggcat gccccttggc gaatccatgc tgaaggcctt
     3661 catgaagata ttccggatcc actaccgtac gggagtaggg gtcagcgaag taacagcgga
     3721 tgaggtggtc ctgagtacgg gtgaaaggat cgcctcctgt ttcaccatgc tgatgccgcc
     3781 gttcgtcggg gtcgatctga tccggaactc gccggagctc ggcgccgaac caaacggatt
     3841 cctggaagtc ctgccctcct accgccaccc caaatatgat aatatctggg tggcggggct
     3901 gaccgtaaat gtaaagccgc cctttacgcc cggagccgta ccgtttggag tgccaaaaac
     3961 gggattcccg agcgatgaga ccgggaagat cgtcgcggag aatattgtac gggtggcaaa
     4021 tggaaggacc gatcttcggg aaaaggcgtg ggggaagatc cccggtttat gcgtcatgga
     4081 cgccggccgg aaggaggtgc tgatcctgtc gaaccatctt ttcaggcccc gcagctttgc
     4141 catcatgctg ccaaacgtgt tttatgacgt gatgaaggtg ctgctggaga aatatttcct
     4201 ctggaagatg cggcatggtt atgcacagct tccctagccg gctctttctt tcccgaatcc
     4261 tgcctacctt cgtccaaagg catggtatga aactactcgt catcgaggat gagcccagtc
     4321 tgcaggccag catcacccgg tatttccggc aggagcaatt cgcgtgcgag agcgttggca
     4381 attacgcgga cgccctgcaa aagatcgatg actgcgaata cgactgtgtc atcctggata
     4441 tcaatcttcc cgggggcagc ggcctgcaat tgctgcagcg actgagggag gacaggaaaa
     4501 aggacggggt catcatcatc tccgcccggg attccctgga cgacaagatc tcaggcctgg
     4561 atcatggggc ggatgactat ctcaccaaac cctttcatct ctccgagctc aacgcccggg
     4621 taaaggcgct gatccgccgc aaatactcgg aaggcagcgc gggcatgcaa gtcggcctgc
     4681 tctatatcga ccttgcggcg cggacggtct ccacgggagg gcagcccgtt gggctaagcc
     4741 agatcgaata ccggctgctg gtattcctgt cggccaacaa gaacagggtg gtaagccggc
     4801 aggctatcgc cgagcatctg catgtcggcc cttcggatca ggcccctggt cccgaattcg
     4861 tttactcgca tatcaagaac ctgaagagaa aactgaagga aaaaggttgc ctcgaaatga
     4921 tccataccgt ttatgggttt ggttataaac tcctgtcatg agcaaacctc tccagcaaag
     4981 aagcacccgt tcgctgctta cctggct
//
LOCUS       DATGFG010000877         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_80568, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000877 DATGFG010000000
VERSION     DATGFG010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80568"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VKQ52_16505"
     CDS             complement(<1..266)
                     /locus_tag="VKQ52_16505"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HLZ88859.1"
                     /translation="MDPSHSKKISTAGLLITLGIIFGDIGTSPLYTFQTILLEGGNAS
                     VELVLGAISCVFWTLTLQTTFKYVFITLKADNKGEGGVFSLYALV"
     gene            complement(516..1046)
                     /locus_tag="VKQ52_16510"
     CDS             complement(516..1046)
                     /locus_tag="VKQ52_16510"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HLZ88860.1"
                     /translation="MIKFKTKILQFKERMGEKTGWSYIKVPAKLAQQLKPDNKRSFRV
                     KGKLDNHAINGMALIPMGEGDFILALKAAIRKAIRKQAGDSLDVQLEVDKKEILPPKE
                     LLECLADEPAAEAYFRSLPKSHQNWYGNWVKAAKTEGTRTRRIAQIVDGMFKKMSFPE
                     MLRAYGETLRSMREDH"
     gene            complement(1064..1906)
                     /locus_tag="VKQ52_16515"
     CDS             complement(1064..1906)
                     /locus_tag="VKQ52_16515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ88861.1"
                     /translation="MKRHLFQTIGLLCLATLSFSQTLPYGSNKDAGRYYPIRGFSMYC
                     EIYGQGQPVLIIHGNGGSISNFNMNIPYFSSKYKVIIADSRAQGRSADPGDSLTYEMM
                     ADDLAALLDQLHLDSAFVIGWSDGGIDGLLLAMRHPEKVKKLAVTGANLWPDTTAVPQ
                     EIWDMVRPDYAKLKEKKAAGLTPQEKTSFKLLRLLCDQPHIALGDLQAIKCPTLVIGG
                     DHDVIKPIHTLTIAENIPHSYLWILPNSGHSTPVAYHDLFNKTVDDFFTRPFREITGE
                     KRFY"
     gene            complement(1962..2510)
                     /locus_tag="VKQ52_16520"
     CDS             complement(1962..2510)
                     /locus_tag="VKQ52_16520"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLZ88862.1"
                     /translation="MKAKESAEIIPTKDVFVGDPKAPVTLMEYGDYESEACAKVNEVV
                     GKLLEQFDGKLRFNFRHFPLTKIHQHAMKAAEASLGAAQEGKFWEMHNTLFAHRRRLG
                     AISLREYAKEVGVVNKKFLDNLVNSTYGWQVRSDLLEGLNKGVRDVPAFFINGVLFTG
                     KPTFENLRKGIEEALKLKKKRA"
     gene            complement(2558..>3392)
                     /locus_tag="VKQ52_16525"
     CDS             complement(2558..>3392)
                     /locus_tag="VKQ52_16525"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLZ88863.1"
                     /translation="FIFLGTTGVGKTELAKALAEYLFDDEGMMTRIDMSEYQEKHTVS
                     RLVGAPPGYVGYDEGGQLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRLTDN
                     KGRVVNFKNTIIIMTSNMGSQIIQANFEDVTEKNREEVMDRTRVEVVDLLRQTIRPEF
                     LNRIDEIILFQPLLKSEIRGIIRIQLDGLRQMVAKNGINLQFSDYAIDYLAENGFDPQ
                     FGARPLKRLIQKEIVNQLSKKILAGDVDRTYPVLVDVFDGVVVFRNEHPHAEKTAEAI
                     K"
BASE COUNT          711 a          950 c          883 g          848 t
ORIGIN      
        1 accagggcat ataaggagaa aacgcctccc tcgcctttat tatcggcctt gagggtaata
       61 aaaacatact tgaaggtggt ttgtaaagta agggtccaga agacacagga gatggcgcca
      121 aggaccaatt ccacgctggc attgcctcct tcgagcagga tcgtctggaa cgtatacagt
      181 ggggaagtac caatatcgcc gaaaataata cctagtgtga tcagaagccc tgcggtagag
      241 atcttttttg aatggcttgg gtccatatgt ggcaccccct cggcaaaaag tgtacctata
      301 acacaaagac cgtcgccgat gagcccaaat ccgctacggc agggcatctc cgctacgact
      361 cctctcaaag gaccaatgac ccggccgcac aaaaccatac caaaatggga aaacccttac
      421 cattttggta aaaggttccg gcagcgggtg aaagatcctt ctacgccagg ttaatcgggt
      481 acaccggatg aatagggtac accaggttaa tcgggctaat gatcttcccg catcgatcgg
      541 agggtctcgc cgtaggcccg gagcatctcc ggaaaactca tctttttgaa catgccgtcc
      601 acgatctggg cgatgcgccg ggtccgggtg ccttccgtct ttgccgcctt tacccagttg
      661 ccgtaccaat tctggtggga tttgggcaaa ctcctgaaat acgcctccgc ggccggttca
      721 tcggccaggc attcgagcag ttcctttggt gggaggatct cttttttgtc cacctccagt
      781 tgcacgtcga gactgtcgcc ggcctgcttg cggatggcct tgcggatcgc ggccttgagt
      841 gcgaggatga aatccccctc ccccatcggg atcagggcca tgccgttgat ggcatgatta
      901 tccagctttc cttttacccg gaaggatcgc ttgttatccg gctttagctg ttgggccagt
      961 ttcgccggaa ccttgatgta ggaccagccg gtcttctctc ccatcctctc cttgaactgc
     1021 aatattttgg ttttgaattt tatcatgacg acagcgcttt cgcttaatag aatcttttct
     1081 cgcccgtgat ctcgcggaag ggcctggtga agaaatcatc cacggtttta ttgaagagat
     1141 cgtggtaggc taccggggtg ctgtgcccgc tgttgggtaa tatccataga taggagtgcg
     1201 ggatattctc cgcgatggtg agcgtatgga tcggttttat cacgtcgtga tcaccgccga
     1261 tgaccagggt cgggcatttg atcgcctgca ggtcgcccag ggcgatatgc ggctggtcgc
     1321 aaagcaggcg cagcaattta aagctggtct tctcctgcgg cgtaagtccg gcggcttttt
     1381 tctcctttag tttggcataa tccggtctga ccatatccca gatctcctgg ggaaccgcgg
     1441 tcgtgtccgg ccagagattg gcgcccgtga ccgcgagctt cttcaccttc tccgggtgac
     1501 gcatcgccag cagaagacca tcgatgcccc cgtcactcca gccaatgaca aacgccgagt
     1561 cgaggtgcag ctggtcgaga agggcggcca ggtcgtcggc catcatctca taggtgagcg
     1621 agtcgccggg atcggctgac ctcccctggg cgcggctgtc ggcgatgatc accttgtatt
     1681 tggaggagaa ataggggatg ttcatgttga agttggagat cgatccgccg ttgccatgga
     1741 tgatcagcac cggctggccc tggccgtaga tctcgcagta catgctgaaa ccccggatgg
     1801 gatagtacct gccggcgtcc ttattactgc cgtaaggcag ggtctggcta aaggaaaggg
     1861 tggccaggca gagcaggccg atggtttgga acaggtgtct tttcatgaac ctaatataat
     1921 aaaaaagccc ccaaaaccgg gggctctttt tacaccttat cctatgctcg tttctttttt
     1981 agtttcagcg cctcttcgat ccccttgcgg agattctcga aggtaggttt tccggtaaat
     2041 agaacgccgt tgataaagaa ggccggcaca tcccgtaccc ccttattcaa cccttccagc
     2101 agatcgctcc tcacctgcca cccataggtg gaattgacaa gattgtccag gaattttttg
     2161 ttcaccacgc ctacctcctt cgcatattca cggaggctga tagcccccag gcggcggcgg
     2221 tgggcgaaga gcgtgttgtg catctcccag aatttccctt cctgggccgc gccaagcgat
     2281 gcctcagccg ccttcatggc atgctgatgg atcttggtca gcggaaaatg ccggaaattg
     2341 aacctcaatt ttccgtcaaa ctgttccagg agcttcccta ctacctcgtt gaccttagca
     2401 caggcctcgc tttcataatc cccgtattcc atcaaggtta cgggcgcttt cggatcaccc
     2461 acgaaaacat ccttcgtagg aatgatctcg gctgattctt ttgctttcat taggtatata
     2521 acatcctgcc gacggttttg ttcggtcaga atgacattta ttttatcgct tccgccgttt
     2581 tttccgcatg agggtgctcg ttccggaaaa cgaccacccc gtcgaagaca tccaccagta
     2641 cggggtaagt gcggtcaaca tcgcctgcca gtatcttctt actcaattgg ttaacgattt
     2701 ccttctggat cagccgtttc aacggccggg caccgaactg gggatcgaaa ccgttctccg
     2761 ccaggtaatc gatggcatag tcgctgaact gcaggttgat gccgttcttc gccaccattt
     2821 gccgcaggcc gtccagctgg atacggatga tccccctgat ctcgctcttc agcaagggct
     2881 ggaacaggat gatctcgtcg atcctgttga ggaactcggg ccggatggtc tgccgcagca
     2941 ggtccaccac ttccaccctt gtcctgtcca tcacttcctc gcggttcttt tccgtcacat
     3001 cctcgaaatt ggcctggatg atctggctgc ccatattgct ggtcatgatg atgatggtat
     3061 tcttgaagtt caccacccta cccttgttat cagtgaggcg gccatcatcc agcacctgca
     3121 acagaacgtt aaatacatcc ggatgcgctt tctcgatctc gtccagcaat atcacgctat
     3181 acggtttgcg tcttaccgct tccgtcagtt gaccgccttc gtcgtaaccg acatagccgg
     3241 gaggggcgcc gacaagccgg ctcaccgtat gtttctcctg gtattcgctc atgtcgatcc
     3301 gggtcatcat gccctcatca tcaaagaggt attccgccag ggccttggcc agttcggtct
     3361 taccgacacc cgtggtcccg aggaagataa ag
//
LOCUS       DATGFG010000878         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_247936, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000878 DATGFG010000000
VERSION     DATGFG010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247936"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VKQ52_16530"
     CDS             complement(<1..320)
                     /locus_tag="VKQ52_16530"
                     /inference="COORDINATES: protein motif:HMM:NF015546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88864.1"
                     /translation="MSIFILNNELLFPPVHLAEPDGLLAVGGDLSIERLLLAYRHGIF
                     PWYEGRHILWWCPDPRFVLFPEELKESKSMKQLHKRNAFEFRTDTAFTEVITNCKTIA
                     RRGQ"
     gene            complement(342..647)
                     /locus_tag="VKQ52_16535"
     CDS             complement(342..647)
                     /locus_tag="VKQ52_16535"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HLZ88865.1"
                     /translation="MPFSLHNPRTIELEDIDVLTATEDPFSLIVWNDEVNTFEWVIET
                     LMEVCGHSQEQAEQCAMIIHTKGKYAVKRGSYDELKPQCDAITDRGIGATIEILSES"
     gene            721..1332
                     /locus_tag="VKQ52_16540"
     CDS             721..1332
                     /locus_tag="VKQ52_16540"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLZ88866.1"
                     /translation="MTPEREQRITSVLDKRQENITIVLENVFDPHNISAVMRTCDAVG
                     VQDLYVLNTRIPRHKKWGAKSSSSAAQWLTIHQYDNTEECFVHLRKRADRIFATHLSS
                     DAVSLYGMDLTGRIALIFGNEHSGVSDEIRALADGNFIIPQVGIIRSLNISVACAVTL
                     YEAFRQKTAAGQYNQPGLNPARYDQLLKEWSRGQLTVPQDNTK"
     gene            1340..1858
                     /locus_tag="VKQ52_16545"
     CDS             1340..1858
                     /locus_tag="VKQ52_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLZ88867.1"
                     /translation="MRVLFILLLFLGSIAVRAGGNDSLPSPSTEVSKMKITELEDLIA
                     HSDHPLIINFWATFCAPCNKEIPYFQATVARYAASKVELVLVSLDLPDYYPARIASFA
                     QSHAYTARLVWLNETDADYFCPKVDQRWTGGIPCSLFINNRTHYRRFFDRQLTEPQVD
                     LEIKKMVAAPAN"
     gene            complement(1855..>2167)
                     /locus_tag="VKQ52_16550"
     CDS             complement(1855..>2167)
                     /locus_tag="VKQ52_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88868.1"
                     /translation="DRNGGNSFEEMQAYARSQGYNWYYALDAKSTLADAFGASRTPEC
                     YLFDKKGVLVYHGAIDDSPGDQLQVKRHHLQVAIDEMMSGKEVTVKETRSVGCSINRG
                     A"
BASE COUNT          489 a          629 c          571 g          478 t
ORIGIN      
        1 tcctggccgc gccgggcgat cgtcttgcag ttggtgatca cctcggtaaa ggcggtgtcc
       61 gtccggaact cgaaggcatt tcgtttgtgc agctgtttca tactcttgct ttccttcaat
      121 tcctccggga agaggacgaa ccgcgggtcg ggacaccacc acaggatatg ccggccctca
      181 taccagggga atatcccatg acggtaggcc agcaggagtc tttcgatgga aagatcgccg
      241 ccaacggcca gtaacccgtc cggttcggcc aggtggacgg gaggaaataa gagttcatta
      301 ttgagaatga agatggacat ggtttgcacc tgctgatgcg cttaggattc cgagaggatc
      361 tcgatcgtcg cgccgatgcc ccggtcggta atggcatcac attgcggctt caattcatca
      421 tacgacccgc gctttaccgc atattttccc ttggtgtgga tgatcatggc gcattgttcg
      481 gcctgctcct gggaatgccc gcatacttcc atcagggtct ctatcaccca ctcgaaggta
      541 ttcacctcat cgttccacac gatcaggctg aatggatcct cggtagccgt cagcacatct
      601 atgtcttcca gttcgatcgt gcgtggatta tgtaaagaaa agggcatggg gcactagctt
      661 ttgacaaaat tacatatttc ggttgcccgt acaagcccta tttttgcact ttattctact
      721 atgaccccgg aaagagaaca aaggattacc agcgtgttgg ataaacgaca ggaaaacatc
      781 accatcgtac tggagaacgt cttcgatccg cacaatatct ccgccgtcat gcgtacctgc
      841 gatgcggtgg gtgtacagga tctgtatgtg ctcaataccc gtatcccccg gcataagaaa
      901 tggggggcaa agagcagcag cagcgccgcc caatggctca cgatccatca atatgataac
      961 acggaggaat gttttgtcca tctgcgaaaa agggccgacc ggatcttcgc cacccatctc
     1021 agcagcgatg ccgtatcgtt gtacgggatg gacctcaccg ggcggatcgc cctcatcttc
     1081 ggcaacgaac attcgggcgt gagtgacgag atccgtgccc tggcggacgg caatttcatc
     1141 atcccgcagg taggcatcat ccggtcgctg aacatttccg ttgcctgcgc cgttaccctc
     1201 tatgaggcct tccggcagaa gaccgcggcg ggacaataca accagccggg tctgaatccg
     1261 gcgcgatatg atcaactgct aaaggaatgg agcaggggac agttaaccgt tccgcaagac
     1321 aacacaaaat agaaccggta tgcgcgtctt attcatcctc ttgttgtttt tgggaagcat
     1381 cgccgtccgc gcgggcggca acgacagtct gccttcgccg tcgacggagg tcagcaaaat
     1441 gaagatcact gaactggaag acctcatcgc gcacagcgat caccccctga tcatcaactt
     1501 ctgggctacc ttctgcgcgc cctgcaataa agagataccc tatttccagg caaccgttgc
     1561 ccggtatgcc gcctcgaagg tggaactggt gctcgtcagc ctggatctgc ccgactacta
     1621 ccccgcacgc atcgcttcct ttgcccagag ccacgcctac accgcgcgcc tggtctggct
     1681 gaacgagacc gatgccgatt atttctgccc aaaggtggat cagcgatgga cgggagggat
     1741 accttgttcg ctctttatca acaacaggac gcactaccgc cggttcttcg accggcagct
     1801 gaccgaaccc caggtagacc tggaaataaa aaagatggtg gcggcccccg ctaactaagc
     1861 accccggttg atggagcatc caaccgatcg ggtctccttc acggtcacct ccttgccgga
     1921 catcatctcg tcgatcgcaa cctgcagatg atgccgcttc acctgcagct gatcgccggg
     1981 ggaatcgtcg atggcgccgt ggtagaccaa tactcctttc ttatcgaaga gataacattc
     2041 gggtgtccgg ctggcgccaa aggcatcggc gagggtactc ttcgcatcca gcgcataata
     2101 ccagttgtac ccctgcgacc tggcataggc ctgcatctct tcaaaggagt tgccgccatt
     2161 acggtca
//
LOCUS       DATGFG010000879         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1547246, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000879 DATGFG010000000
VERSION     DATGFG010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547246"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VKQ52_16555"
     CDS             <1..371
                     /locus_tag="VKQ52_16555"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HLZ88869.1"
                     /translation="TTTLNTNNIPTGKSLLVIGFSPWCKHCQAETQDIVKHIDSLKNT
                     RILFVTPYPFDQMRTFYRYFKLSQYPNITMGRDTKDYFLTYFKAEGVPYTAIFDSKKR
                     LIQAIANEAHASDLVHAAAE"
     gene            complement(373..957)
                     /locus_tag="VKQ52_16560"
     CDS             complement(373..957)
                     /locus_tag="VKQ52_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ88870.1"
                     /translation="MKTSIKGDDWFLLFREGDREAFRLVFDKYYRSISYFASKILRDD
                     GFAEDVVSETFRKAWDRRDKFATPRHLENFLYLVTRNGCISYLRSDRVIQSTAQEWVR
                     IAGDKEAGESSLDLERVQTKLLEVIFEKLEELPGGNVLRMAFLEGKSTQEIASELHIT
                     ENNVYIIKSRSLKALRTLLTKNEWMFFVLLFLRY"
     gene            complement(1181..2476)
                     /locus_tag="VKQ52_16565"
     CDS             complement(1181..2476)
                     /locus_tag="VKQ52_16565"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HLZ88871.1"
                     /translation="MKRVLILLAFLPVFGMSQGGVHFEEGLTWQQVKDKAKAEHKYVF
                     VDCFATWCGPCKQMDKNVYPMDSVGDVVNSNFVSVKVQCDTSKDDNESAKAWYADAHE
                     IVSEYKITAYPTFLFFSPEGKIVHQGLGFQKPEAFAKLANEALDPQKQYYTLVENYQQ
                     GKRDFAVMPTLSAAAQQFKDTSLSRVIAEDYIYNYLNRLPEASFCTRDNFVFIGTHVT
                     TVSSKARIFDWCFRRPAEVDTILHDKGFSDRLVNFVVYKEEIAPSMSAAKISGRTPNW
                     EAIGSAIRKKFGAAYVENNILNAKIGWYRSVKDWKNYTKYLVAKMDKDDVQKNVPAGF
                     WGFVGLNASAWDVFEHSDNKMELEKALTWSNIVITKIEPKSGPNYGTFMDTKANLLYK
                     LGKKEEALELEAKASALTPNDKQVQEAYKKMQEGKPTWE"
     gene            2854..3120
                     /locus_tag="VKQ52_16570"
     CDS             2854..3120
                     /locus_tag="VKQ52_16570"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HLZ88872.1"
                     /translation="MKSLLHRLIRLDYLIHLKSTGTPANCANKIGISERSLYDYLKIL
                     KEMGAPIKFSRNRGTYYYDEDGRFRISFISKYSDQTASEEMLAS"
     gene            complement(3117..4382)
                     /locus_tag="VKQ52_16575"
     CDS             complement(3117..4382)
                     /locus_tag="VKQ52_16575"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HLZ88873.1"
                     /translation="MQKILLTLLLSFLFCSIFGQAGRGYSPGYTFLQSGNIVLDKDSY
                     LITAIGQSSKVKNLLAGDDSLAGILQKQVALIRAHISDTCRTAESLVSGFRWRKEDSL
                     RVDGAFRRIYGRQPAPFDALVDGQLRPSGYYQRFVGSGNLDLLLRAWGQCVVGINYIL
                     DQYGLGRKLRYPAIDSVSYDVRGEYYQDLLKALFPFLHERADSMTLFVQPSLALAWEL
                     MRANDRDEPARFEPLEQGENRAATARVKTLDWSRYKYTAILVPGEGPELATVPFDPIG
                     RMRCDLAADRYKKGLAPFIIVSGGYCHPFHTPYCEAIEMKHYLTREWGIPEAAVIIEP
                     QARHTTTNFRNACRLIIRYGMPVERPSLCVTTKDQADYIDDPRFGNRCRRELGYLPYR
                     DLRRTSDHEFTFLPVMECLHMDPYDPLDP"
     gene            4512..>4747
                     /locus_tag="VKQ52_16580"
     CDS             4512..>4747
                     /locus_tag="VKQ52_16580"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HLZ88874.1"
                     /translation="MKRSFLADSISFFFIFLFLYTGVLKLTEIQTFRQQLSSSPIMAS
                     LAGVITWALPIGEILLAIALFIPRWRLKALYVTAG"
BASE COUNT         1195 a         1315 c         1062 g         1175 t
ORIGIN      
        1 gtactaccac tctgaatacc aataatattc ccaccggcaa atccctgttg gtcatcggat
       61 tttcgccctg gtgcaagcat tgccaggcgg aaacgcagga tatcgttaag cacatcgatt
      121 ccttgaaaaa tacccgcatc ctgttcgtaa ccccttatcc ttttgaccaa atgcgcacct
      181 tctaccgtta cttcaaatta tcacagtatc ccaatatcac catgggtcgc gacaccaagg
      241 actatttcct cacctatttc aaggcggaag gagtacccta tacggccatc ttcgattcca
      301 aaaagagatt aatacaagcc atagcaaatg aagcgcacgc ttccgacctg gtacatgcgg
      361 cagcggaata gatcagtaac ggaggaaaag caggacaaag aacatccact cattcttggt
      421 gagtaaggtc cggagggcct tcagcgaccg ggatttgata atatagacgt tgttctcggt
      481 gatgtgcagc tcagaggcta tttcttgtgt gctcttacct tccaggaagg ccatccggag
      541 cacattgcct cccggtagtt cctccaactt ttcaaagatc acctcgagaa gcttggtctg
      601 tacgcgttcg aggtccaggg aagactctcc agcttcttta tctccggcta tccgaaccca
      661 ttcctgggcc gtgctttgaa taacgcggtc gctgcgcagg taagaaatgc agccgttgcg
      721 cgtaacaaga tagaggaagt tctccaggtg gcggggagtg gcgaatttgt cccttcggtc
      781 ccatgccttg cgaaaggtct cgcttacaac gtcttcggca aagccgtcat cgcgtagtat
      841 ctttgacgca aaataactga tcgaccggta atatttatca aataccagcc ggaatgcttc
      901 cctgtcccct tcccggaaga ggaggaacca atcatcgcct tttatactcg ttttcatgct
      961 cgggtaagca aatcttcgcc tacttaacta caggttgtaa aaatttgaca atcaatattt
     1021 gaaataggaa agggcggtat ctcaaggttg gatcggacaa ctcgaattac aagatacaaa
     1081 aattccagca aaaaaatgcg ccgcagtaaa gcaaacgccg gcgcagtaat agtcactgcg
     1141 ccggcgcata aatttacggc cggtaaggta tccggtttcg tcattcccag gttggctttc
     1201 cttcttgcat tttcttatac gcttcctgca cctgcttatc attgggggtt aaggcagatg
     1261 ctttcgcctc gagctcgagt gcttcttctt tcttgcccaa cttatataaa aggttggcct
     1321 tagtatccat aaaagtgccg taattcggcc cggacttggg ctctatcttc gttatgacaa
     1381 tattactcca ggtcagcgcc ttctccaatt ccattttgtt gtcactatgc tcaaatacat
     1441 cccatgcgct ggcattcaag ccaacaaatc cccaaaaacc tgcaggcaca ttcttctgca
     1501 cgtcatcttt atccattttg gctaccaggt atttggtata gtttttccaa tctttaacag
     1561 accgatacca gccaattttg gcattcagga tattgttctc gacatacgcc gcaccaaact
     1621 tcttccttat agccgaacca atggcttccc agttgggcgt cctcccgcta attttggccg
     1681 ccgacatcga gggcgctatt tcttccttat atacaacaaa gtttaccaac cggtcggaaa
     1741 aacccttgtc atgcaggatg gtatctacct cagcgggacg acgaaagcac cagtcgaata
     1801 ttcttgcctt tgaacttacg gtagtaacat gagtgccgat aaacacaaaa ttatctcttg
     1861 tacaaaagga tgcctcgggc agtctgttaa ggtaattgta gatgtaatct tcggcaatca
     1921 ccctggagag gctcgtgtcc ttgaattgct gggcggcagc ggagagagtg ggcatgacgg
     1981 cgaaatccct tttcccctgc tggtaatttt ctaccagcgt ataatattgc ttttgagggt
     2041 caagtgcctc atttgccaac ttcgcaaacg cctccggttt ctgaaagcct agcccttgat
     2101 gtactatctt cccctccggc gaaaaaaaaa gaaaggtcgg gtaagcagta atcttgtact
     2161 cggatacaat ttcatgcgca tccgcatacc atgcctttgc cgactcgtta tcatccttcg
     2221 acgtatcgca ctgcaccttc accgaaacaa aattgctgtt aacaacatct cccacactat
     2281 ccatcggata cacattttta tccatttgct tacacggccc gcaccaggtg gcaaaacaat
     2341 cgacaaaaac atacttatgc tccgccttag ccttgtcctt cacctgctgc caggtcaaac
     2401 cttcctcgaa atgcactccc ccctgggaca tgccaaaaac aggcaagaat gccaataaga
     2461 tcaatactct tttcatttta agttgaattt tcagaaatgc aataaattga ctatcaattt
     2521 tctagtcaaa acaagaatta gatagccatg cccaaactgc aattactacg cagtcttttt
     2581 acatttcttt caacaggctc atcctgagca catttactaa acgggaatca cgaatgggcc
     2641 atccttccag gaacaaaatc ctgtctttag tgcaccaaca aaatttgctc ccaaccccta
     2701 tcgcatcgta acttacggtt acagacaacg ggaatctctc catacatcaa ggctctggtt
     2761 ggcagcatcc cacaagcaca actctacatc cctctcggtt agcagcacca caaggcattc
     2821 cttttccaat tataaaagca aaaaaccatt gccatgaagt ccttattgca ccgccttatc
     2881 cggctcgact acctcatcca ccttaaaagc accggcacgc ccgccaactg cgccaacaaa
     2941 atcggaatct ccgaacgcag cctctacgat tatctgaaga tcttaaaaga aatgggcgct
     3001 ccgatcaaat tctcgcgcaa ccgggggacc tattattatg acgaagacgg caggtttcgt
     3061 atctccttta tttccaaata ctcagatcaa acggcatcgg aagaaatgct tgcttcttag
     3121 ggatccaggg gatcataggg gtccatatgc aaacactcca tcaccgggag aaaggtaaat
     3181 tcatgatcgg atgtccgccg caggtcgcgg tagggcaggt agcctagttc ccggcgacag
     3241 cggttgccaa atcgtggatc atcgatataa tccgcttggt ccttcgttgt cacgcataga
     3301 gagggtcgct ccacgggcat cccataccgg atgatcagcc ggcaggcatt gcggaaatta
     3361 gtggtggtat gccgggcctg gggctcgatg ataacggctg cctcggggat tccccattcc
     3421 cgggttagat aatgcttcat ctcgattgcc tcgcaatacg gcgtatgaaa gggatgacag
     3481 tacccgccgc tgactataat aaagggggcg agaccctttt tatagcggtc ggccgcaagg
     3541 tcacagcgca tccgcccgat cgggtcaaag ggaacggtag ccaattcggg gccttcgcca
     3601 ggcaccagga tcgccgtata cttgtaccgg ctccagtcga gggtcttcac ccgcgctgtc
     3661 gcggccctat tctccccctg ctccagcggt tcgaagcgcg caggttcgtc ccgatcattg
     3721 gcgcgcatca gttcccaggc aagagcgagg gaaggctgga caaacagcgt catggaatcg
     3781 gcccgctcgt ggagaaaggg aaacagggct ttgagcaggt cctggtaata ttccccccgg
     3841 acgtcgtaac tcaccgaatc gatagcgggg taacgcagct tcctccccaa cccgtattga
     3901 tcaaggatat aattgatccc cacgacacat tgcccccagg cgcggagcag cagatccagg
     3961 ttgccagaac ctacaaaccg ctggtagtac ccggatggcc ggagctgacc atcgaccagc
     4021 gcatcgaaag gcgcgggctg ccggccatag atacgccgga acgccccatc cacccgcagg
     4081 gagtcctcct tgcgccaccg aaacccgctt accaggcttt cggccgtccg gcaggtatca
     4141 cttatatggg ctcggatcag cgcgacctgt ttctgcagga tgccggccag ggagtcgtct
     4201 cccgccaata ggttctttac ttttgacgat tgaccgatgg cggtgatgag gtaagaatcc
     4261 ttatccagca cgatattccc ggattgcagg aatgtatacc caggcgaata cccgcggcct
     4321 gcctggccga agatcgagca gaacaggaac gaaagaagga gcgtcagcaa tatcttttgc
     4381 atataaggca ttacggatca atgcgctgct taaccaatga tctttccctg atgggcctac
     4441 cttttgggga catttttatc cttttcgaga atagcatccc gcctaacttg gacctgttta
     4501 taccttatta tatgaagcgt agtttcctcg ccgatagcat ctcctttttc tttatcttcc
     4561 tattcctcta tacaggggtt ctcaaactga ctgagatcca aaccttccgg cagcaactat
     4621 cctcttcccc catcatggca tccctggccg gcgtcatcac ctgggccctc ccgatcggag
     4681 aaatactgct ggcgatcgcg ctgttcatcc cgcgttggcg gctgaaagcc ctatatgtga
     4741 ccgccgg
//
LOCUS       DATGFG010000880         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_691546, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000880 DATGFG010000000
VERSION     DATGFG010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691546"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..539)
                     /gene="ytxJ"
                     /locus_tag="VKQ52_16585"
     CDS             complement(207..539)
                     /gene="ytxJ"
                     /locus_tag="VKQ52_16585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HLZ88875.1"
                     /translation="MNWIKLQSENQLEEIRERSVSRPQLIFKHSTRCSTSALVKGRLE
                     RAQQPEAIDFYYLDLISHRPVSNRIAETFFVEHESPQVLLIRNGECVYDESHLGITMA
                     DIVDASAA"
     gene            complement(626..751)
                     /locus_tag="VKQ52_16590"
     CDS             complement(626..751)
                     /locus_tag="VKQ52_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88876.1"
                     /translation="MRKTIVILVIATVIATAFTSCATSYSSRSGCKMSQGYVGYR"
     gene            1020..2375
                     /locus_tag="VKQ52_16595"
     CDS             1020..2375
                     /locus_tag="VKQ52_16595"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HLZ88877.1"
                     /translation="MKVHFIAIGGSVMHQLAIALKRKGYAVTGSDDEIFEPARTNLQK
                     EGLLPASTGWEPARIQPGLDAVILGMHAKGDNPELQRALELSLPIYSFPEYIYRESRD
                     KKRVVVGGSHGKTTTTAMIMNVLAAAGMDFDYLVGARLEGFAQSVNITHAPVIVCEGD
                     EYPASALEKRPKFHFLFPHIAVITGIAWDHINVFPTFDNYLEQFRIFIGMIEPDGVLI
                     YNDTDPVLKKLVTEHPSTVRRIGYGIPGYRIENGITLVTLEGQTGALKVFGEHNLLNL
                     HAAFLVAKELGLDAGDFLGGMASFSGAAKRLELIGAKDGVNVYRDFAHAPSKVKATIQ
                     AVKMQFPDRQLIAVLELHTYSSLNEQFLSEYKGALDPADKAVVFYSRHALELKRMPPL
                     PEEKVVAGFGKPGLVVLQEKEELQAWLMQQSYKNVNLLLMSSGNYDGVDVIAFSNQIT
                     Q"
     gene            2397..3167
                     /locus_tag="VKQ52_16600"
     CDS             2397..3167
                     /locus_tag="VKQ52_16600"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLZ88878.1"
                     /translation="MLQLTRPLAIIDLETTGVNLGTDRIVEIAIVKIMPDGKKLIKRK
                     LINPEIPISSTSIDIHGITNEMVKDAPTFRQVANELKQFLDNCDLAGYNSNRFDIPML
                     VEEFLRIGMDFDGRGRKLLDVQKIFHMMEQRTLSAAYRFYCQKELEGAHGAEADASAT
                     WEVLVAQVSKYPQLGTNIDTILKCIGEEPVVDFARRFVLENGVEVFNFGKHKGRSVAD
                     ILKAEPQYYDWMMKGDFPLHTKQKLTEIFNRTMLKKGQ"
     gene            3221..>3507
                     /locus_tag="VKQ52_16605"
     CDS             3221..>3507
                     /locus_tag="VKQ52_16605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ88879.1"
                     /translation="MEKIVIIPTYNERENITHILEAIFQLGQGFHVLVIDDSSPDGTA
                     DIVRQLQARYPGQLFLEERHGKLGLGTAYIHGFKWAVRNNYPFIFEMDADFS"
BASE COUNT          902 a          730 c         1036 g          839 t
ORIGIN      
        1 tggctaagga tggcggggtt tacgacaatg atataagaag tagccagaaa ggaggagaat
       61 ccggccagga tctcggtaga cgggtttacc tggtgtcggc cgtgggccag gtcctggcta
      121 aaattgaaaa agcggggcat atccggtcgt ttggaccgga agatacataa ataaatgagt
      181 ggggctacgc catccaatga cctgggctac gccgccgaag catcgacaat gtcggccatg
      241 gtgatgccga ggtggctttc atcgtatacg cattccccgt tgcggatgag caggacctgg
      301 ggggattcgt gttcgacaaa gaaggtctcg gctattctat tcgatacggg ccggtggctg
      361 atgaggtcga gatagtaaaa atcgatggcc tcgggctgtt gggcgcgttc caggcggcct
      421 ttgacgaggg cgctggtgga gcaacgggta ctgtgtttga agatgagttg ggggcgggag
      481 accgatcgtt cgcgtatctc ttccagctgg ttttccgact gcagtttgat ccaattcata
      541 gccaatataa caagaaacaa taaaaaaagg ttatcgattg ataacctgca aaatacaata
      601 tttttccggt agggcggggg gcagactagc gatatcctac ataaccctgg ctcatcttgc
      661 aaccgctgcg ggagctatag gaagtcgcgc aggaagtaaa tgcagtagcg atgaccgtgg
      721 cgataactaa aattacaatt gtctttctca ttttttgtgg tatttaagat tgaaacgata
      781 aattgcctgg ttgaaaggaa tgtggaccag gtaattcaaa ggaagggata cggaggggaa
      841 ccacaggttc ggggccgagg gccaggacat taacaaggcg aaacacagtt tcgactgccg
      901 aaggccagga cattaaatta aggggagatt ttcgaaagga tagagaaaga attaatcggg
      961 gaactggcac aaagtaaaca caaaaaatcg atattcgcaa gccctaaggg gttataatta
     1021 tgaaagttca ttttattgcc ataggtggga gtgtcatgca tcagttggcc atcgccctta
     1081 agcgtaaagg atacgcagtg acggggagtg atgatgagat cttcgagccg gcgcgtacca
     1141 acctgcaaaa ggaagggttg ctgccggcct cgaccggctg ggagccggcc cgtatccagc
     1201 cgggtctgga cgcggtgatc ctgggaatgc atgcgaaggg agataacccc gagctgcagc
     1261 gggcgctgga gctgtcgctc ccgatctatt ctttccctga atatatttac cgggagagcc
     1321 gggacaagaa acgggtagtg gtaggcggca gtcacggtaa gacgaccacg actgctatga
     1381 tcatgaatgt gctcgcggct gccggaatgg actttgatta tcttgtcggc gccaggctgg
     1441 aggggtttgc tcaatcggtg aatatcaccc atgccccggt gatcgtctgt gagggggatg
     1501 aatatcctgc cagtgcgctg gagaagagac ccaaattcca ttttcttttt ccacatattg
     1561 cggtgatcac gggtattgcc tgggaccata tcaatgtctt tccgaccttt gataattacc
     1621 tggagcagtt caggatcttt atcggcatga tcgagccgga tggggttctg atctataacg
     1681 acaccgaccc tgtgctaaag aagctggtga cggagcatcc ctccacggtg cggaggatcg
     1741 ggtatggaat tcccggatac aggatcgaaa acggcataac gcttgttaca ctggaggggc
     1801 aaaccggggc gctaaaggtc tttggcgaac acaatctttt gaacctgcac gcggctttcc
     1861 tggttgccaa agagttaggg ctcgatgccg gggatttttt aggaggtatg gcctcttttt
     1921 cgggcgctgc caagcggttg gagctgatcg gcgcgaagga cggtgtcaat gtctaccggg
     1981 atttcgcgca tgcgccgtcg aaggtaaagg ccacgatcca ggcggtgaag atgcagttcc
     2041 cggaccggca gttgatcgcc gtgctggagc ttcatacgta tagcagtctg aatgagcagt
     2101 tcctttcgga gtataagggg gcccttgatc cggccgacaa agcagtagtt ttttattccc
     2161 ggcatgcgct tgaattaaag cggatgccgc ctttacccga ggaaaaagta gtggcggggt
     2221 ttggaaaacc gggtttggtg gtcctgcagg aaaaggaaga attacaggcc tggctgatgc
     2281 aacaatctta caaaaatgtt aatttgcttt taatgagctc cggtaattat gatggggtgg
     2341 atgtaatagc cttttccaac cagattacgc agtaggagtt tttataatat tatcgaatgt
     2401 tacaacttac acgcccctta gcgatcattg atcttgagac aacgggggtc aatctcggga
     2461 ctgaccggat agtagaaatc gccatagtga agatcatgcc ggatggaaaa aagctgatca
     2521 aacggaagct gatcaacccg gagataccta tttcaagtac ctcgatcgat attcatggga
     2581 tcacgaatga aatggtgaag gatgcgccca cctttcggca ggtggcgaac gagttgaaac
     2641 aattcctgga taattgtgac cttgcgggat ataattccaa tcggtttgac atacccatgc
     2701 tggtggaaga gttcctgcgg atcgggatgg atttcgacgg caggggcagg aagctgctgg
     2761 atgtacagaa gatctttcat atgatggagc aacggacgct gagcgccgct taccggttct
     2821 attgtcagaa ggagttggag ggtgctcatg gagcggaggc ggatgcttcg gccacatggg
     2881 aggtattggt ggcccaggtg tcgaaatatc cacaattggg aacgaatatt gataccatcc
     2941 tcaaatgtat aggcgaagag ccggtggtgg attttgcccg gcgctttgtg ttggagaacg
     3001 gggtggaagt atttaacttc ggcaagcata agggtcgatc ggtggccgat attctgaagg
     3061 ccgaacctca gtattatgat tggatgatga agggtgattt cccgctccat accaagcaga
     3121 agctgaccga gatatttaac aggacaatgt taaaaaaagg gcaatagctg ggtagcgccc
     3181 tgaaaatttg taattttgta gcacatatat aatcaggcag ttggaaaaga tcgttatcat
     3241 acctacttac aacgaaaggg aaaatatcac ccatatcctg gaagctattt ttcagttggg
     3301 acaaggtttt catgtcctgg ttatagatga cagctcgccg gatggaacgg cggatattgt
     3361 ccgtcagtta caggcgagat acccgggcca gctctttctc gaagaacggc atggtaagct
     3421 ggggctgggt acggcgtata tccatgggtt caagtgggct gtccgtaata attacccctt
     3481 tattttcgag atggatgccg atttctc
//
LOCUS       DATGFG010000881         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_154811, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000881 DATGFG010000000
VERSION     DATGFG010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154811"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VKQ52_16610"
     CDS             complement(<1..1387)
                     /locus_tag="VKQ52_16610"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88880.1"
                     /translation="MLKNFFKVAFRNMARNKAHSFINITGLSVGMAVAMLIGFWVYDE
                     LSFNTFFDSHDRIAVVVRNFNYNSQRISLPGYCPYQLREELLNKYAADFRSVVLSTGT
                     GDHLIANSNTNFQKKGSFMDAGAADMLSLRMLKGSRAGLQQPSGLLLSASVAATIFGN
                     ADPLGRTLQIDGQSNAVVTGVYEDIPYNTDFKDLGFIASLQLFITANPIIKTSKSPWM
                     DASFFTAYVQVTDRADMSSVSLKIKNILLGELPDGEYKIEQPYMFLQPMDKWHLYADF
                     ENYTGYKVREKIEYVRLMGVIGIFVLLLACINFINLSTARSEKRAKEVGVRKTIGSLR
                     TQLIGQFLFESLLAVGFAFLLSLLWVVLALPSFNRIADKHIAIPWGVPFFWIACVLFG
                     LLTGLISGLYPALYLSSFEPVKVLKGTFRLGPLAAIPRKILVVFQFTVSVGLIICTTV
                     IYRQIQFAFDRP"
     gene            1752..2204
                     /locus_tag="VKQ52_16615"
     CDS             1752..2204
                     /locus_tag="VKQ52_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88881.1"
                     /translation="MSKPIFFAMLFAIVVAPFWLFKLGWVMRSKKAQGVMAFAGMGFA
                     GDQIKEDYSVISFRVGEDRIWFNGLGNLPYKPGDKLGVRYQPGDVYDVRVDTFAGIWG
                     DTLVYSGIPALILLVLFLHPLVVPWGSRIRLTARKPFIGVHSPEKLHR"
     gene            2201..>2410
                     /locus_tag="VKQ52_16620"
     CDS             2201..>2410
                     /locus_tag="VKQ52_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88882.1"
                     /translation="MNRIIIFGSGILAALAFIAVRVLDRHHSPLTDFTAYLLFPIFCL
                     VFLFSLFLVRIRRIGSSPLTGNIIRG"
BASE COUNT          599 a          586 c          621 g          604 t
ORIGIN      
        1 tgggccggtc gaatgcgaat tggatctggc ggtagatcac cgtcgtacag atgatgagtc
       61 ctaccgacac ggtgaattgg aaaacgacca ggatcttgcg ggggatggca gccagcgggc
      121 ccaaacggaa ggtgcctttc aagactttta ccggctcgaa agaggagaga tacagcgccg
      181 gataaaggcc ggagatcagc cccgtcaaca ggccgaagag gacacaggcg atccaaaaga
      241 aggggacccc ccagggaatg gcgatatgct tgtccgctat ccggttgaaa gagggaaggg
      301 caagtaccac ccagagcagg gataaaagaa aggcaaagcc caccgccagc agggactcga
      361 aaaggaactg accgatcaat tgggtgcgaa gagagccgat cgtcttgcga acacctacct
      421 ctttggcgcg cttctccgag cgggctgtgc ttagattgat aaaattgata caggccagca
      481 gcaatacaaa aatgccgatg acacccatca gccggacgta ttcgatcttt tcccgaacct
      541 tgtatccggt gtaattctcg aagtccgcgt acaaatgcca tttgtccatc ggctgcagga
      601 acatgtaggg ctgttcgatc ttgtattcac cgtcgggaag ctcgcccagc aaaatattct
      661 ttatttttaa cgatacgctg ctcatatccg cccggtccgt cacctggaca taggcggtaa
      721 aaaagctcgc atccatccag gggcttttgg atgtttttat gatggggttg gctgttataa
      781 acagctgcag tgaagcgata aaaccgaggt ccttgaaatc cgtgttgtag gggatgtctt
      841 catacacgcc cgtcaccacg gcattcgatt gtccgtctat ttgcagcgtt ctccccaggg
      901 ggtcggcatt gccaaaaatg gtcgccgcaa ccgatgcgga caaaagaagg cccgatggct
      961 gctgtaatcc tgcccgtgat cctttcaaca tccgcaggct aagcatgtct gccgccccgg
     1021 catccataaa actgcctttt ttctgaaaat tggtgttgga attggcaata agatgatcgc
     1081 ctgtcccggt tgagaggacg acggatcgaa agtcggctgc atatttgttc agcaattcct
     1141 cccgcagttg ataaggacag tagccgggta aggagatcct ttgggaatta taattgaagt
     1201 tccggacgac tacggctatc cggtcatggg aatcgaaaaa ggtattgaac gataactcat
     1261 catagaccca aaacccgatc aacatggcta ccgccatacc caccgacaac cctgttatat
     1321 tgataaagga atgggcttta ttcctcgcca tattgcggaa agcgaccttg aagaagtttt
     1381 tgagcataag aaagggtttg actttggcct ggtccggaca gcgcccggaa ccattcggtc
     1441 aaagctaccg ctaaagtcag aaacaattcc ggcttttacc aatttttaca gcaacacgca
     1501 acgaaaccaa attaggttga agtaattgtt tttctttagt agcactactg tttttttttg
     1561 cgggttctac taatatagtc aaatataatt tgtaactgga tgattgcctg attgaaacga
     1621 ggatgtaatt gacaacacgc acagtactgc aagatccagg agggaatatt tattttcaat
     1681 acatcgtttt cttatgtatc tttatgttag gcattaggaa acttttcctg atccgcatag
     1741 aaaacatgat catgtccaaa cccatcttct ttgcgatgct ctttgccatc gtcgtcgcgc
     1801 cgttctggct gttcaaactc ggatgggtga tgcgttcgaa gaaggcgcag ggtgtcatgg
     1861 cttttgcggg gatggggttt gccggcgacc agataaagga ggattattcg gtcatctctt
     1921 tcagagtagg ggaggatagg atctggttca acgggcttgg caatctaccc tacaaaccgg
     1981 gagacaaatt aggcgttcgc taccagccgg gcgacgtata cgatgttcgg gtggacacct
     2041 ttgccgggat ttggggagat acgctggtgt acagtggcat ccctgcgctg atattgctgg
     2101 ttttgttcct gcacccctta gtcgtaccct ggggctcgcg gatcaggctg actgccagga
     2161 agccctttat cggggttcat tctcctgaaa aactccatag atgaacagaa taataatttt
     2221 cggcagcggc atcctggcag ccctggcctt cattgccgta cgtgtcctgg acaggcatca
     2281 ctcgccactc acggacttta ccgcgtatct tctttttccc atcttttgcc ttgtatttct
     2341 cttttccctt ttcctggtgc gcatccggcg catcggttca agccccctga cgggaaatat
     2401 cattcgcggg
//
LOCUS       DATGFG010000882         5683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1072419, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000882 DATGFG010000000
VERSION     DATGFG010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072419"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VKQ52_16625"
     CDS             <1..433
                     /locus_tag="VKQ52_16625"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ88883.1"
                     /translation="TFNDDEYAQKFATETRIGTLAAVFSGLAILISCLGLFGLASFVA
                     EQRTKEIGVRKILGAGVVNLWALLSGDFIRLVILSMAIAMPLIGLAMHSWLQNYTIHT
                     ALSPWIFISAGAGILLITLVTVSFQAVRAALTNPVKSLRSE"
     gene            complement(542..1831)
                     /locus_tag="VKQ52_16630"
     CDS             complement(542..1831)
                     /locus_tag="VKQ52_16630"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ88884.1"
                     /translation="MKKLLLIPTLLWACLLFAQAPSSKTIIYCGRLIDPRSGQVLTEM
                     SVVVTGNSITDVKKGYIAAAGDDKTIDLKNRTVMPGLIDSHVHLEDAFGPNSQLNRFT
                     KSTADIAYESIGYAKTTLMAGFTTVRDVGGSGVNIALRNAIRKGLVVGPHMLTAGKII
                     SSTGGHGDPTHGYRDGLYTEPSFTETVADGKDACIKAVRQMYKEGADLIKITASGGVL
                     SLEKDGSGAQFSDEELKAIVSTAKDYGMPVAAHAHGAEAMKRAIRAGVTSIEHGTFMD
                     DETIALFKQYGTWYVPTIIAGKSVADSAKKPGFFPPVIAGKAIAIGPVIQGTFAKAYK
                     AGVKIAFGTDAGVYMHGKNWMEFVYMTEAGMPALEAIRCATVNASELLGLSDKTGTIE
                     PGKWADIIAVEGDPTKDIHAMGQVRFVMKEGVVYKSE"
     gene            complement(1946..4876)
                     /locus_tag="VKQ52_16635"
     CDS             complement(1946..4876)
                     /locus_tag="VKQ52_16635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLZ88885.1"
                     /translation="MKKYARVLSILLLFLPFFSQAQAPPIAWQRCLGSPYLDFGWSVE
                     PTSDGGSIVVGYVGGNGGDVSNYHGNISVNDYWVAKLDNKGTIQWSRTLGGTFFDEGS
                     VVRQAPDGSFIVAGEAASLPGDGDVTTPNKGGTDYWMVKLSSTGNVLWEKSFGGERNE
                     YLYDLRFTADGGYILTGETESTGGDVTGNHGGRDAWVVKVNTDGSLGWQKCIGGSQDD
                     ESYSIQVRTDGYVLSGYTTSSDGNISGYHGGTDMLVAKLDLAGNLVWCKSLGGSGYDL
                     GWGIDNSPDGGTIVSGQTGSGDGNVSGIHGGAGPRDAWVVKLDVNGALQWQQCYGGSF
                     NEAAFSIAATPDGGYVFAGSAQSMDGDVTCMPANVSQRGLQAGWVVKITSTGGMQWEK
                     VISGGYYDEMHSVRPTADGGYLVAGYTGLNSLPSYHKDQDPNGGVGDFYIAKLKMPTV
                     TINQPPANICSGAPVTFTATVSDYLDNILYQWIRNLSNQGVGTPTYTATNFANGDQVY
                     ARILISDGTCNALIPVLSNTVTLSVSPLTAPTVSIAGNAGPVCAGTPVTFTATARGGT
                     PAGVYQWMLDGQPVGGNSVTYNTSGLGDGDIISCVYTDNTVCAVPGLITSNVITERVD
                     PGVNVTVSIAADATTVCAGAPVHFTATPVGGGNAPGYQWLLNNNPVGAGSPVYTSVTP
                     ASGDVVSCQLTSSATCSTPNPATSNAVTLTVNPVLASSVSIAYSPTEICSGKPVLFTS
                     TASNAGAAPSYQWAVNGQAVGSGGASYRSSTLADGDVVSCDLTGAGCVTPSTTSVTLQ
                     VFPTPAVGTVPPILLSKGQSVPLNLPVSGDVATYSWSPVTGLSDPTVADPVATPLKTT
                     TYTLTVVSPNGCVDSGQMTVKVFSKLSIPAAFSPNGDGHNDIFYVIGGPLGSVIGDFV
                     VFDRWGSIVFQVHGVAPDDPAFGWNGTVDGRPAPTGTYVYEIRMVLAGGEQQVVKGTV
                     VLVR"
     gene            5167..>5683
                     /locus_tag="VKQ52_16640"
     CDS             5167..>5683
                     /locus_tag="VKQ52_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88886.1"
                     /translation="MRNIYICIILITLYFNPAFKVFAQTYRLLESSSTLKGPKHEILP
                     RPNGDILTITHPNTGDKGPMTVSRFDDQLNEIYTRRITLLSHECYQAAWYDNDRLFLF
                     CTDKLGGLTRYEIDENTGSLTGQPAPLTGLLGIVSHPKNAAFLTGNSPDGAFHYIVSS
                     MEGKHSAEKTIR"
BASE COUNT         1200 a         2027 c         1362 g         1094 t
ORIGIN      
        1 taccttcaac gacgacgaat atgcgcagaa attcgccacc gagacccgta tcggcaccct
       61 ggccgccgtc ttctccggat tggctatcct tatctcctgt ctcgggctct tcggtctcgc
      121 ctccttcgtg gcggagcaac ggacaaagga gatcggcgtc cgcaagatcc tgggcgcagg
      181 cgtcgtcaac ctctgggccc tcctctccgg cgacttcatc cgcctggtca tcctctcgat
      241 ggctatcgcc atgcccctca tcggcctggc catgcacagt tggttgcaga actacaccat
      301 ccacaccgcc ctctcgccct ggatcttcat cagcgccggc gccggcatcc tgctgatcac
      361 cctcgtgacc gtcagtttcc aggcggtgcg ggcggctctt accaatcccg tcaaaagcct
      421 gcggagtgaa taaattgcag caccgcctgg cgccccgaca atagatagat tacgatccga
      481 ataactccgc ggcccggcga cacaatcgat tggcctccgg ccatcggcgc cgggccgccc
      541 cctattcgct cttatacacc accccctcct tcatcacgaa ccgcacctgc cccatcgcat
      601 ggatatcctt cgtgggatcg ccctcgacgg caataatgtc cgcccacttc cccggctcga
      661 tcgtccccgt cttatcgcta agcccaagca gctccgacgc attcaccgtc gcacacctga
      721 tcgcttccag cgccggcatc cccgcctcgg tcatatacac gaattccatc cagttcttcc
      781 catgcatata tacccccgcg tccgtcccaa aagcgatctt caccccggcc ttgtacgcct
      841 tcgcaaaggt tccctggatc accggaccga tggcaatagc cttccccgcg atcaccggcg
      901 gaaaaaaacc cggcttcttc gccgaatccg ccaccgactt gccggcaatg atcgtcggca
      961 cataccaggt tccatattgt ttgaacagcg caatggtctc gtcatccatg aacgttccat
     1021 gctcgatcga ggtcaccccc gcacggatcg cccgcttcat cgcctcggcc ccatgcgcat
     1081 gcgccgccac cggcatccca taatcctttg ccgtcgagac tatcgccttc agctcctcat
     1141 cggagaactg cgccccgctg ccatccttct cgagactgag caccccgccg ctggccgtga
     1201 tcttgatcag atcggcgcct tctttataca tctgccgcac agccttgata caggcatcct
     1261 ttccatcggc gacagtctcg gtaaaagagg gttcggtata caacccatcc cggtatccat
     1321 gcgtgggatc accatgccca cccgtggaag agatgatctt tcccgccgta agcatatgcg
     1381 gccccaccac gagccccttc cggatcgcat tccgcagcgc gatattcacg cccgagccgc
     1441 cgacatcccg taccgtggtg aaacccgcca tcagcgtcgt cttcgcataa ccgatagact
     1501 cataggcgat gtccgccgtg ctcttcgtga agcggttcag ctgggaattt ggcccgaagg
     1561 catcctcgag atggacatgc gaatcgataa gccccggcat caccgtccgg ttcttcagat
     1621 cgatggtctt gtcatcaccg gccgcggcga tatacccctt cttcacatcg gtgatgctgt
     1681 tgccggtgac caccaccgac atctccgtca gcacctgccc cgacctggga tcgataagcc
     1741 ggccacaata aataatggtt ttggatgaag gggcctgcgc aaacagcaga caggcccata
     1801 atagcgtggg tataagcagt agtttcttca tacacccaat atacgacatt ccgttcacag
     1861 ccatccatcg ccccgaacac cgcaacccgc ctccgtcctt gcgcccgcgg cacccttacg
     1921 ccgcgcccac tatcactgcc gcccgctacc gcaccaacac caccgtccct ttcaccacct
     1981 gctgctcccc acccgcaagg accatcctga tctcatatac gtaggttccc gtcggagccg
     2041 gccgcccatc caccgtcccg ttccagccaa aggccggatc gtcgggcgcc accccatgca
     2101 cctggaatac gatacttccc caccggtcga acaccacaaa atccccgatc acgctcccaa
     2161 gcgggccgcc aatgacataa aagatatcgt tatgcccatc tccatttgga ctaaacgccg
     2221 ccgggatcga caatttcgag aacaccttta ccgtcatctg cccgctatcc acacagccgt
     2281 tcggcgacac cacggtcagc gtgtaggtcg tcgtcttcag tggagttgcg acagggtcgg
     2341 ccaccgtagg gtcggacaac cccgtgacag gactccagct ataggttgcc acatcccccg
     2401 acaccggcag gttcagcggg acgctctgcc ccttcgacag gaggatcggc ggcacagtac
     2461 cgaccgctgg cgtcgggaac acctgcagcg tcacgctggt ggtggaagga gtaacacaac
     2521 cggccccagt gagatcacag ctcaccacat ccccatcagc cagcgtcgaa ctccggtagc
     2581 tcgccccccc gctgcccacc gcctgcccgt tcaccgccca ctgataggac ggagcggcgc
     2641 ccgcattgct ggcggtcgag gtaaagagca cgggcttgcc cgaacagatc tccgtgggtg
     2701 aataagcgat gctcacggaa gaggcaagca ccgggttaac cgtcagcgta acggcattac
     2761 tcgtcgccgg gttgggagtg ctgcaggtgg cgctgctcgt cagctgacaa ctgacgacat
     2821 cccccgacgc aggcgtcacg ctcgtataga cgggactgcc ggcccccacc gggttattat
     2881 tgagcagcca ttgatacccc ggcgcattcc ccccgcccac cggcgttgcg gtaaagtgaa
     2941 cgggcgcgcc ggcacagacc gtcgtcgcat ccgcggcaat gctcaccgtt acattcacgc
     3001 cggggtccac ccgctcggta ataacattgg aggtgatcaa ccccggaacc gcacaaaccg
     3061 tattgtcggt atacacacag gagatgatat ccccatcccc gagcccgctg gtattgtacg
     3121 tcacgctatt cccacccacc ggctgcccat ccagcatcca ctgataaacc cccgcggggg
     3181 taccgcccct cgccgttgcc gtaaacgtta ccggggttcc cgcacatacc ggaccggcat
     3241 ttcccgcgat actcaccgtc ggcgccgtca acgggctaac ggaaagggtt accgtattac
     3301 taaggacggg gatcaatgca ttacaggttc catcggagat caggatccgt gcgtacacct
     3361 ggtcgccatt ggcaaaatta gtggcggtat aggtgggggt gcccaccccc tggttggaaa
     3421 gattgcgtat ccattgatag aggatattgt cgagatagtc ggaaacggtt gccgtgaaag
     3481 tcaccggcgc accgctacag atattcgcgg gcggctggtt gatcgtcacc gtcggcatct
     3541 tcagcttcgc gatataaaaa tccccgacac ccccattcgg atcctggtcc ttatgatagc
     3601 tcggcagact gttcagtccg gtatagcccg ccaccaggta tcccccgtcc gcggtgggtc
     3661 gcaccgagtg catttcatcg taatacccgc cgctgatcac cttctcccat tgcatgcccc
     3721 ccgtcgacgt gatcttcacc acccacccgg cctgcaggcc ccgctgggag acgttcgcgg
     3781 gcatacaggt gacatcccca tccatactct gcgccgaccc ggcaaacaca taaccgccat
     3841 ccggcgtggc agcgatagaa aaggccgcct cgttaaaact gccgccataa cactgctgcc
     3901 attgcagggc gccattgaca tccagcttca ccacccaggc atcccgcggg cccgctccac
     3961 cgtgaatacc gctgacatta ccgtcaccgg aacccgtttg cccggaaacg atcgtcccgc
     4021 cgtcagggct gttatcgatg ccccagccaa gatcatatcc actgccgcca agactcttgc
     4081 accagacgag attccccgcc aggtccagct tggcgaccag catgtccgtg ccgccatgat
     4141 atcccgaaat attaccatca ctgctggtgg tatagccgga aagcacatac ccatcggtcc
     4201 gtacctggat gctgtacgac tcatcatcct ggctgccgcc gatacatttc tgccagccca
     4261 ggcttccgtc ggtattcacc ttcaccaccc aggcatcccg tccgccatga ttgcccgtaa
     4321 cgtcgccacc cgtggactcg gtctcgccgg taaggatata gccgccatcc gcggtaaacc
     4381 ggagatcgta gagatattca ttcctctctc ccccaaaact tttttcccat aggacgttgc
     4441 ccgtgctgct caatttgacc atccaatagt ccgtgccgcc cttattcggc gtcgtcacat
     4501 ccccatcacc cggcagcgag gccgcctcgc ccgcaacgat aaaactgcca tccggggcct
     4561 ggcgcacaac cgacccttca tcgaaaaagg tgccccccag ggtccgcgac cattggatcg
     4621 ttcccttatt atccagcttc gccacccaat aatcgttgac ggagatattc ccatggtaat
     4681 tgctgacatc cccgccgttg ccgccgacat agccgacgac gatcgacccg ccatcggacg
     4741 tcggttcgac agaccatcca aagtccaggt aaggactgcc gagacagcgt tgccacgcga
     4801 tcggcggcgc ctgggcttgc gaaaaaaaag ggagaaacag gagcaggatg gataggactc
     4861 gggcgtattt tttcatgcag gcttgaggtc agttgagttc agtgggcgct attggataag
     4921 tctaaataaa ggctctatgg gaacaaatct atacaaataa gcccttattt gcaatcggtc
     4981 ccatccccgc cgcgccaaaa ctcccccatc cgcccccgcc agatcctcct gccaccacag
     5041 ctcgtccggc cgaccaatta cccccaccgc gccaaaaccc cgcccgcccg ggacaatacc
     5101 ggcgcccccc gaaccgttat aaacaaaggc ggcaacgccc acacacccct aacaaaatcc
     5161 gaacccatga gaaatatcta catttgtatc attctcatta ccttatactt caatccagcc
     5221 ttcaaggtct tcgcccagac ctaccgcctg ctggaatcct cctccaccct caagggccca
     5281 aaacacgaga tcctcccgcg cccgaacggg gacattctca ctattaccca tcccaacacg
     5341 ggcgacaagg gtcccatgac ggtcagccgc ttcgacgacc aattgaacga gatctatacc
     5401 cgccggatca cccttctcag ccacgaatgc taccaggccg cctggtacga caacgaccgg
     5461 ctcttcctgt tttgcaccga taaactcggg ggcctcaccc ggtacgagat cgacgagaac
     5521 accggctccc ttaccggcca gcccgcaccg ctcaccggcc tcctggggat cgtatcccac
     5581 ccgaagaacg ccgccttcct aacgggcaat tcgcccgacg gcgccttcca ttatatcgta
     5641 tcctccatgg aaggaaaaca ctcggcggag aagaccatcc gcg
//
LOCUS       DATGFG010000883         8345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_166541, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000883 DATGFG010000000
VERSION     DATGFG010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166541"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..463
                     /locus_tag="VKQ52_16645"
     CDS             152..463
                     /locus_tag="VKQ52_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88887.1"
                     /translation="MKEYYHYTLEKYLSSIRKDGIYPNHPYFTTTQYYNASEAGQMLG
                     VMAHNIDCVLKFNDDGNFRPVQNVPGTGRFSGGGNQYSHPFRPKPIAIRKINTRDWVQ
                     L"
     gene            614..871
                     /locus_tag="VKQ52_16650"
     CDS             614..871
                     /locus_tag="VKQ52_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88888.1"
                     /translation="MLGVISVGEYCKTVLLVLGVYYLIMANKLFPGKLRMARRWIGAK
                     IGGGGKEGAGTGRGKNGDSSTEQVPGGEATVSEADGGGSER"
     gene            1407..1751
                     /locus_tag="VKQ52_16655"
     CDS             1407..1751
                     /locus_tag="VKQ52_16655"
                     /inference="COORDINATES: protein motif:HMM:NF024961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4134 domain-containing protein"
                     /protein_id="HLZ88889.1"
                     /translation="MRKSRVMPAWAWGAPGRRGVFIVLGLLGSVAGWAQTADGGTGID
                     QANTMVRSYFDPAVNLMYAIGAVVGIAGAVKVYQKMSHNHPDTERIAMGWFGACIFLV
                     VVATVLKAFFGL"
     gene            1748..2065
                     /locus_tag="VKQ52_16660"
     CDS             1748..2065
                     /locus_tag="VKQ52_16660"
                     /inference="COORDINATES: protein motif:HMM:NF024960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4133 domain-containing protein"
                     /protein_id="HLZ88890.1"
                     /translation="MKSKEFTINKGVNRPLMFRGLKAQYVTYLAFGMLGLLLAFVILH
                     LAGLPTYFCVVTILGAGVLLINRVYTFSRKWGQYGLQKRQAARRLPTAIRSRTRRIFI
                     TGQ"
     gene            2086..5202
                     /locus_tag="VKQ52_16665"
     CDS             2086..5202
                     /locus_tag="VKQ52_16665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032991 - protein-containing complex
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraG family conjugative transposon ATPase"
                     /protein_id="HLZ88891.1"
                     /translation="MEKTWEEVLPLYGIEEDCILSKQGDYTIGYRMIRPEIFTLSGTD
                     YETLLESYTRGMRVLFPGCIIHTQDWYLKDAFHQDPQPGRTWLAEASDRFFEGRPYLN
                     HEAFLFITRRGRRWEGSSGRSGLIFSGGVPKDALDKQAVIEFQEVCDQFAHILESSKL
                     VMLERLKESELRSGPDKAGLIERYCTLATGKVPILKDVRMERGLQIGDEYCQLFTLGD
                     AEDMPGVCSPWVNHDVYSTDKTTFPIGFASKLGPLLNCNHICSTYIFIDSPQEVLSKM
                     EARRRRLQSLATYSRENAASQEAIHEFLTEAVTDQQLPVRIHSNVLTWTDDPYVIPTL
                     KNQASAALAQMGASPRLETVGAPQIWFAGIPGNAADFPMNETSHVFANQAACFLQMET
                     NYRDSAGPFGLRLGDRISGRPLLVDIDKEPKAAGITGNGNMFVLSGSGGGKSFLINHL
                     LREYHDRGVHAVMVDVGHSYRAQCELHGGYYFTYEESAPIHFNPFYPILTEGMDTEKK
                     ESLKTLLLALWKKADETANRAEYVALSSALQAYFDRIRSGADVFPCFDSFYEFMKTGF
                     REELENAGAKEKEFDLDNFLYVLGPYYRGGEFDYLLNAKENLDLLGQRFIVFELDNIK
                     DHPILFPVVTLIIMELFVSKMRKLEGVMKVICIEEAWKAIASSGMAAYMKYLVKTVRK
                     FNAKVVVVTQEVEDIVSSPIVRNAIVNNSDIKILLDQSKFQNKFTEIQELLGITDKQK
                     TEILSINKGHSPGSRYKDLWIGLGATHSKVYRLEVSPEEYFLYTSDQQEKVMVREYSR
                     RWGGIQEGIRRLVADLKGGTLKSGQILVTVLLVLLPGWAPAQDPVSIISEGITKVIKA
                     VDLHIQRIQTETIWLEEAQKVVENAMSQLDLDDIQEWVEDQKDLYGEYFQELKEVKTV
                     IAYYHSISEMIALQEKILASYRSAYGLLSGDTHFTAAELAHISAVYAGMIGESGTLLQ
                     DLQLAVQSLSTQMTDEQRLAIINRARTGMEKVYTDMQRFSNQNILLSVQRGGDAGEIN
                     LLKKLYGL"
     gene            5199..5741
                     /locus_tag="VKQ52_16670"
     CDS             5199..5741
                     /locus_tag="VKQ52_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88892.1"
                     /translation="MKKIIVLALVMGVFVAGRAQTFDEWFEQKKTQIRYLKAQIAALA
                     EYEGVTAKGYTIAQDGTDAISGIKQVDLQLHSDYFSSLRTVNPAIRGSPEVAGTIRLA
                     GVIAEVAAEIGDAEVGLLADCAENVDLLEMLVTDGQVEMTDAERIKAIDILYARMRRL
                     LQTAIAIRDQIGLLHLNLSS"
     gene            5738..6373
                     /locus_tag="VKQ52_16675"
     CDS             5738..6373
                     /locus_tag="VKQ52_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88893.1"
                     /translation="MRRYVVWGWLTLLLAIGLRSRAQSFEAEQLLLDWQKLSQEKQIL
                     QDLYNGYTILSKGYGAIRDLSKGSFDLHKAFLDGLLSVSPTVKTYKRVADIIRLQEQI
                     VSQYRSAWGLIRQDEHFTPDEVGFIGNVYTGLFDRSVRNLTDLAQLLTDGVYRASDAE
                     RLEEIDGLYRQMQGNQVFLMAFTDRTELLALQRGADASDYEVVRGWYGLNG"
     gene            6384..7352
                     /gene="traJ"
                     /locus_tag="VKQ52_16680"
     CDS             6384..7352
                     /gene="traJ"
                     /locus_tag="VKQ52_16680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraJ"
                     /protein_id="HLZ88894.1"
                     /translation="MDSLQGLQGVLDQLYASMLPQCSELIGVGRAIAGFAALWYIAVR
                     VWRDIAHARSVDVFPLLRPFAIGMVLLLYPAVIGVLNGILQPTVTGTAQMNDNANAAI
                     ATLLQQKEEALKNTVNYQMYVGSDGEGNEALWEAYSGNAGSMSPFSGITNAFQFAMAK
                     AYYNLKNAIKEWLSEVLQVVYEAAALCINTIRTFQLLVLAILGPLVLAFSVFDGFQSS
                     LQGWLARYIHVYLWLPVASIFGAIIANIQVNMLQLDISQIQASGGTYFSATDTGYLIF
                     LIIGIAGYFTVPTVAGYIVRAGGGSSLGARVMQMGTAMAAKAVGKL"
     gene            7372..8001
                     /gene="traK"
                     /locus_tag="VKQ52_16685"
     CDS             7372..8001
                     /gene="traK"
                     /locus_tag="VKQ52_16685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraK"
                     /protein_id="HLZ88895.1"
                     /translation="MFQTFKNIDQHFRQLRTVTIVAVLASVVVSGIAIYASHRFALGL
                     QNRIYLLADGKVLEAYASDRRDNVPAEARDHVRTFHELLFTMDPDEKLINANAGRAMY
                     LADGSGKQIFDNLRESGYIAAVVSGNISQAVTVDSIRLDMDSHPYHFHCFATETIIRM
                     TSVTIRRLETDGWLREVSRSDNNPHGFLIEKLVVLDNRDIRAQNRTYEK"
     gene            7991..>8345
                     /locus_tag="VKQ52_16690"
     CDS             7991..>8345
                     /locus_tag="VKQ52_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88896.1"
                     /translation="MKNKIWDFLRFLGSHPMLMVFAALIVYGMCTLAGTGLRRVMLNG
                     LPSSGVFRVPPEERLRAPQSGGAVAQPEGGSRVSRVADDSGGRQAIIPAGRDLAAIRA
                     FEHWMDSLRADPVGRQ"
BASE COUNT         1955 a         1783 c         2752 g         1855 t
ORIGIN      
        1 atttgttacg gtaacctatt gcagttggat tgttcaggta atcctgtcca taatactagc
       61 gatttttgtg ttaagccatg actaggccga cgcgttcggt cttggaggga gttaaaaaca
      121 aaccgtgtca gtgaaataaa ataaatcaaa aatgaaagaa tattatcatt acactctgga
      181 aaaatatctc tcaagtatcc gaaaagatgg gatttatccc aaccatccat acttcactac
      241 tactcagtat tacaatgcct cggaggccgg gcaaatgctt ggcgtcatgg cgcataatat
      301 cgattgtgtt ttaaaattta atgatgatgg aaattttagg cctgtccaga atgtgccggg
      361 aacaggccgt ttttcaggtg gtgggaacca atatagccat ccttttagac cgaaaccgat
      421 agcaataaga aaaattaata cgcgggactg ggttcaattg tagtccggag aaagatccag
      481 aataagccga aaaaaatatt tctatatagt atgtacatac tatgcaaatg cctcttttta
      541 aaagaggcat ttttgttttg tcgaagggat tcgcgggatt gcgggtcggg agacggcaaa
      601 atgaaaatga tatatgttag gggttatatc cgtgggggag tattgcaaga cggtattgct
      661 ggtgctgggc gtctattacc tgataatggc taacaaatta ttcccgggaa agttgaggat
      721 ggcgcgaaga tggatcgggg cgaagatcgg gggtggcggg aaagaggggg cagggacagg
      781 gagaggaaag aatggagatt cgtcgacgga gcaggttccg ggcggcgaag caacagtttc
      841 ggaagccgac ggaggaggct cggagcgatg acggaggagg ttcgggcgct gacggaggtt
      901 tggacagcga cgcaggaggt tcgagcgctg acggaaaagg ttcggggtgg cgcggcgacg
      961 gaggaggttc ggacgttgaa gggggaggtt cgggacggcg acggaggaga tttggggcgt
     1021 cgagagggag gtcaggggcg ctgaaggaga ggagagatga aggaaaggag cgtctaaggt
     1081 ggggagataa taaaatgaga ctttcagggg ttaggttgaa cggttagcgg acaggttggg
     1141 ggacgattag aaagataagt tagggagacg attagggaga cgattaggga gacgggttag
     1201 gtttaacgat taggcggaca ggttggaggg acaggttagg gcgacgagtt aggggacgat
     1261 taagggacat gttgaggacg ggttggggta acgctgcctt acgaccggcg ggcacgagta
     1321 caggaacgcc acggaagggc atgtgggatg acgtcgggtc ggggcagcgt ttttcgagtg
     1381 tattttctaa ctataatata aggaaaatga gaaagagtcg ggtgatgccg gcatgggcct
     1441 ggggggcgcc gggccggcgg ggggtattca ttgttttggg attgctagga tcggtggcgg
     1501 ggtgggcgca aaccgcggat ggcgggacgg ggatcgatca ggcgaacacg atggtgcgaa
     1561 gttatttcga cccggcggta aacctgatgt atgcgatcgg cgcggtggtg ggcatcgccg
     1621 gcgcggtaaa ggtgtaccag aagatgtcgc ataaccatcc cgacaccgaa cggatcgcga
     1681 tgggctggtt cggggcgtgt attttccttg tcgtagtggc aacagtattg aaagctttct
     1741 tcggactatg aaaagcaaag aattcaccat caacaaagga gtcaaccgcc cgctgatgtt
     1801 ccgcggacta aaggctcagt atgtgaccta cttagccttt gggatgctcg ggctgctttt
     1861 ggccttcgtc atccttcacc tggcgggcct gccgacctat ttctgcgtgg taacgatcct
     1921 cggtgcggga gtcctgctga tcaaccgggt ctacaccttt agccggaaat ggggacagta
     1981 cgggttgcag aagcgacagg cggcgaggag attgccgacc gccatacggt cgcgcacgag
     2041 aagaattttt ataaccggcc aatgaggggc cgaaaatgaa gcgttatgga aaaaacctgg
     2101 gaagaagtac tacccttgta cggtatcgaa gaggattgta tcctctccaa acaaggcgat
     2161 tataccatcg gttaccggat gatacggccg gagatcttta cactgtcggg aacagactac
     2221 gagaccttac tggagagcta tacgcgggga atgcgcgtgc tgtttcccgg ttgcattata
     2281 cacacccagg attggtattt gaaagatgcc tttcaccagg acccgcaacc ggggagaacc
     2341 tggctcgccg aggcaagcga tcgatttttc gaggggaggc cttacctgaa ccacgaggcc
     2401 ttcctgttca ttacccgcag aggaaggagg tgggaaggga gcagcggcag atcgggactg
     2461 atcttttcgg gcggagtccc caaggacgcc ttagacaagc aggcagtgat agaattccag
     2521 gaggtttgcg atcaattcgc ccatatattg gagagcagca aattggtgat gctggaaaga
     2581 ttgaaagaat ccgagttgag gagcggcccg gataaggcgg ggctgatcga acgatactgc
     2641 acgctggcga cagggaaagt gcccatccta aaagatgtcc ggatggaacg agggttgcag
     2701 ataggcgacg aatactgcca actgttcaca cttggcgacg ccgaagacat gcccggagtg
     2761 tgcagtccat gggtgaatca tgatgtgtac tctaccgaca agacgacatt tccgattggc
     2821 tttgccagca agttgggacc cttgttgaac tgcaatcata tttgcagcac ctatatcttt
     2881 atcgactccc cacaggaggt gctcagcaag atggaagccc ggcgaaggcg ccttcagtca
     2941 ctggctacct attcccggga gaacgcggca agccaggaag cgatacacga gtttcttacg
     3001 gaggccgtga cggaccagca actaccggtg agaatccatt ccaatgtgtt gacctggacg
     3061 gacgatccct atgtcatacc gaccttgaag aaccaggcga gtgcggcgct ggcccagatg
     3121 ggggcaagcc ccaggctgga aacggtgggc gcaccccaga tatggttcgc gggtatcccg
     3181 ggcaatgcgg ccgatttccc catgaacgag acctctcatg tatttgccaa ccaggcggcc
     3241 tgttttctac agatggagac aaattatcgg gattcggcgg gtccatttgg gttgcggctc
     3301 ggagaccgga ttagcgggcg gccgttgctg gtggatatcg acaaggagcc gaaagcggcg
     3361 gggatcaccg gcaatggaaa tatgttcgtg ctgagcgggt cgggcggtgg caagagcttt
     3421 cttatcaacc acctgctgcg ggagtaccat gaccgtgggg tacatgccgt gatggtcgat
     3481 gtggggcata gctaccgcgc gcaatgcgag cttcacgggg gctattattt cacctatgag
     3541 gagagcgccc cgatacactt caatcccttc tatcccatcc tgacggaggg gatggatacc
     3601 gagaagaagg aaagcctgaa gacgctgttg ctggcgttgt ggaagaaagc cgacgagacg
     3661 gcgaaccgcg cggaatacgt ggccttatcg agcgccctgc aagcctattt cgaccggata
     3721 cgatcgggag cggatgtctt tccgtgtttt gatagttttt atgagttcat gaagactgga
     3781 tttcgcgagg aattggagaa tgcaggagca aaggagaagg agttcgacct ggataacttc
     3841 ttgtatgtgc tggggcctta ttaccgtggc ggggagttcg attacctgct aaatgcgaaa
     3901 gagaatcttg accttctcgg tcagcggttc atcgtcttcg agttggataa tatcaaagac
     3961 cacccgatac tcttccccgt ggtgacactg atcatcatgg agctgtttgt ttcgaagatg
     4021 cggaagctgg agggggtgat gaaggtcatc tgcatcgagg aggcctggaa ggccattgcc
     4081 agtagcggca tggcggccta tatgaagtat ctcgtaaaga cggtacgaaa attcaatgcc
     4141 aaggtggtgg tcgtgacgca ggaggtggag gacatcgtgt cctctcccat tgtgcggaac
     4201 gccatagtca ataactccga catcaaaatt ctgctggatc agtccaaatt ccagaacaag
     4261 ttcacggaga tacaggagtt gctggggatc accgataagc aaaagaccga gatcctgagc
     4321 atcaataagg gacattcgcc ggggagccgg tataaggacc tgtggatcgg gcttggcgcc
     4381 acgcactcga aggtataccg ccttgaggtc tccccggagg agtatttctt atacacttcg
     4441 gatcagcagg agaaggtgat ggtgcgggaa tattcccgtc gatggggcgg tatccaggaa
     4501 ggtatccgcc ggctggtagc cgaccttaaa ggcggcacgc ttaagagcgg acagatcctt
     4561 gtaacggtgt tgctggtgct cctgcccggg tgggcgccgg cccaggaccc ggtgtcgatc
     4621 atttccgaag gcatcaccaa ggtgatcaag gccgtggacc tgcatatcca gcgtatccag
     4681 acggagacga tctggctgga agaagcacag aaggtggttg agaatgccat gagccagctg
     4741 gaccttgacg atatccagga atgggtggag gatcaaaagg acctgtacgg ggaatatttc
     4801 caggaattga aggaggtgaa gacggtgatc gcctattacc attcgatctc ggaaatgatc
     4861 gcgctacagg agaagatatt ggcgagctac cggtccgctt atgggttgct cagcggagac
     4921 acgcatttca cggcggcgga gctggcgcat atcagcgcgg tctatgccgg gatgatcggc
     4981 gagtcgggga ctctcctgca agacctgcaa ctcgcggttc aatcgctttc cacccagatg
     5041 acggatgaac aacggctggc cattatcaac cgggcgagga ccggcatgga gaaggtgtat
     5101 acagatatgc agcgtttcag caaccagaat atcctgctca gcgtgcagcg gggtggcgat
     5161 gcgggagaga tcaatttgtt gaagaaatta tatggtttat gaaaaagata atcgtattgg
     5221 cgttggtgat gggcgttttc gtggcaggcc gggcgcagac gttcgatgag tggttcgagc
     5281 aaaagaaaac ccagatcagg tatctgaagg ctcagatagc ggcattggcc gaatacgaag
     5341 gagtgacggc aaagggatat accatcgccc aggatggaac ggacgcgatc tccggcatca
     5401 agcaagttga tctccaattg cacagtgatt atttttcgtc gttgcggact gttaatccgg
     5461 cgattcgcgg ttcaccggaa gtggcgggga cgattcgact ggcgggggtg atcgcggagg
     5521 tggcggcgga gatcggagat gcagaggtgg ggctactagc ggattgtgcg gagaatgtcg
     5581 atctgttgga gatgcttgtc accgatgggc aggtagagat gaccgatgcc gagcggataa
     5641 aagcgatcga tattttatac gcgcggatgc ggcggcttct gcaaacggcg atcgcgatac
     5701 gggatcaaat tggtttactc catttaaatc tttcatcatg agacggtatg ttgtttgggg
     5761 ctggcttaca cttttgctgg ccatcgggtt gcggtcgagg gcgcagagct tcgaggcgga
     5821 gcagttgttg ctggactggc agaaattatc acaggagaag cagatactac aggatctgta
     5881 taacgggtat acgatcctat ccaaagggta tggggcgata cgggacctca gcaaggggag
     5941 ttttgacctg cacaaggctt ttctggacgg gcttttgtcc gtgagcccga cggtaaagac
     6001 ctataagcgt gtcgcggata tcatccggct gcaggaacag atcgtgagtc agtatcggtc
     6061 ggcctggggg ctgatccggc aggatgagca ttttacgccg gatgaggtcg ggtttatcgg
     6121 gaatgtctat acggggttgt tcgaccggag cgtcaggaac cttaccgatc tggcccaact
     6181 gctgacggac ggggtgtacc gggcgagtga cgcggaacgg ctggaggaga tagacgggct
     6241 ctaccggcag atgcagggga accaggtgtt tctcatggct tttaccgatc ggacggagct
     6301 actggcctta cagcggggcg ccgacgcgag tgattacgaa gtagtgaggg gatggtatgg
     6361 attgaacgga taaatcgttt tttatggaca gtttgcaggg tttacaggga gtgctggacc
     6421 agttgtatgc cagtatgctg ccgcagtgca gcgagttgat cggggtgggc cgggccattg
     6481 cgggttttgc ggcgctgtgg tatatcgcgg tgcgcgtgtg gcgggatatt gcgcatgcga
     6541 ggtcggtgga cgtgtttcct ttgctccgtc cttttgcgat cgggatggtc cttttgcttt
     6601 atcccgctgt gatcggggtc ctgaacggca ttttgcagcc gacggtgacg gggactgcgc
     6661 agatgaatga taatgccaat gcggcgatcg ccacgttgtt gcagcaaaaa gaagaggccc
     6721 tgaagaatac cgtcaattac cagatgtatg tcgggagcga cggggaaggc aatgaggcgc
     6781 tttgggaggc atattcgggg aatgcaggga gcatgagccc attttcgggg atcacgaatg
     6841 cttttcagtt tgcgatggcg aaggcctact acaatctcaa gaatgcaata aaggaatggt
     6901 tgtcggaggt gttgcaggtg gtgtatgaag cggcggcgct ttgtatcaat acgatccgga
     6961 ccttccagct gctggtgctg gcgatattgg ggccgctggt gttggcgttc tcggtgttcg
     7021 acggtttcca gtcgagctta cagggttggc tggcgaggta tatccatgtc tatctctggc
     7081 tgcctgtagc cagtatcttc ggggccatca tcgccaatat ccaggtgaac atgctgcaac
     7141 tggatatcag ccagatccag gcgagcggcg ggacctattt cagcgcgacg gatacgggat
     7201 acctgatctt cctgatcatt ggcatcgcgg gatactttac cgtgccgacc gtggcgggat
     7261 atatcgttcg tgccggcggc ggttcgtcgt tgggggcgcg ggttatgcag atggggactg
     7321 cgatggcggc gaaggcggta gggaaattat aagttttaaa ccagtgaatc tatgttccaa
     7381 acatttaaaa atatcgatca gcatttccgg caattacgaa ccgtgacgat cgtggcggtg
     7441 ctggcgagtg ttgttgtcag cgggatcgcg atctatgcca gtcaccggtt tgcgctaggc
     7501 ttacagaacc ggatctacct gttggcggat gggaaggttt tggaggctta tgcgagtgac
     7561 cggcgcgata atgtcccggc ggaggcgcgc gatcatgtgc ggaccttcca tgagctgttg
     7621 ttcacgatgg acccggatga gaagctgatc aatgccaatg cggggcgggc gatgtacctg
     7681 gcggatgggt caggcaagca aatctttgat aaccttcggg agtcggggta tatcgcggcg
     7741 gtggtgtcag ggaatatcag ccaggcggtg acggtggaca gtatccggct ggatatggac
     7801 agtcatccgt accatttcca ttgtttcgcg acggagacga tcatccggat gacgagtgtg
     7861 acgatccggc gcctggagac ggatggctgg ctgcgggagg tgagccggag tgataataat
     7921 ccacatggat ttcttattga aaagctggta gtcctggata accgggatat acgggcgcaa
     7981 aacagaacgt atgaaaaata agatttggga tttcctgcgg tttttgggca gtcatccgat
     8041 gctgatggtg tttgccgcgt tgatagttta tgggatgtgt acgctggcgg ggaccgggtt
     8101 aagacgggtg atgttgaatg gtctgccgtc atcgggggtg ttccgcgtcc cgccggagga
     8161 gcgtttacgg gctccgcaga gtggtggtgc agtcgctcag ccggaaggag gctcgcgggt
     8221 ttcgcgggtg gcggatgatt cgggtgggcg acaggctatc attccggcgg gccgggatct
     8281 ggcggcgatc agggcttttg agcattggat ggatagtctg cgggcggacc cagtgggccg
     8341 gcaaa
//
LOCUS       DATGFG010000884         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_46858, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000884 DATGFG010000000
VERSION     DATGFG010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46858"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VKQ52_16695"
     CDS             <1..1188
                     /locus_tag="VKQ52_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ88897.1"
                     /translation="MDNQVYYSHYFFNLISNFTFYYFYPATGDEFRQHERRDLYGYDG
                     KITHTAGLGAGSLTSSAGWGVRYDDVSPSFLAHTLNGDSLLNYIQLGQMRETAMYGSL
                     DETLRSGKWLVDAGVRLDYLHFYYGNMAPATDTSAAIYAGVSPRAGRAIVSPKITIQY
                     SANPRVQLYVKLGKGFHSNDARIVVARQGYEILPAAYGADLGLNWKPLPRLFVNAALW
                     WLYLQQEFRYGADLGDQAVSPGGRTRRTGIDFSARYQFNDWLFGNLNVDLARPRDLDA
                     KMGENFLPLAPGFTSTAALDFRLASGWNGGISYRYLRNRPANAGNTLTAPGYFVSDGS
                     VNYTRKRWEMGLTIENLFNQRWNESQFDYVSRLKDERTPVDEVSYTPGAPFFARLKFT
                     VFF"
     gene            complement(1298..2269)
                     /locus_tag="VKQ52_16700"
     CDS             complement(1298..2269)
                     /locus_tag="VKQ52_16700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HLZ88898.1"
                     /translation="MKAIIITHPGGPDVLQVQDRETPQPAGDEVLIEVKASGLNRADL
                     FQRGGNYPAPPGVPADIPGLEVSGIITRCAPAVTQFKQGDKVCALIAGGGYAQFVTAR
                     EGQCLPIPANLDFIEAAALPEAIGTSWSNIFQRGRLTAGENLLIHGGNSGIGTAAIQL
                     GKAFGATVYVTVGSEEKGNACLSLGAHRFINYKTQDFAVELADIGIDVILDMVGGEYW
                     KKNIRILREEGRLVYINAVAGNTVDLNLHQMMARRLTITGSTLRVRDYAWRKALSTEI
                     KEKVWPIIAAGRYKPVIYRTFPYTEAAEAHREMEAGRHTGKIMLSWE"
     gene            complement(2343..>3807)
                     /locus_tag="VKQ52_16705"
     CDS             complement(2343..>3807)
                     /locus_tag="VKQ52_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HLZ88899.1"
                     /translation="VRWVGNEEGHAGTTCWETFTPEAPDPGKKPSNGYSKYWQATSGT
                     RNGEFWMPAECDVPLRPGWFYHASQDAQVKTPYQLLDLYYQSVGRGAGLDLGLAPNRD
                     GLLDSHDVAALQQFGALLKQIFTVNLAKDATFTASNIRGGNRSLYGPGRLVDGDRYTY
                     WATDDNLTTPQLTITLGKPTVFNVIRLRENIKLGQRIDSFAVDTWENNDWKLIAAGTS
                     IGACRLIRLQQNVTTSKLRLRIVSAPVCIALSDFGLYKEPTHLVPPTITRDKAGMVGI
                     TTSGPVGSIHYTLDGSEPTLQSPAYQSPFEQKGDGAVKARSFESADAHSETTTKEFGL
                     SKGEWKIVFPATTAGTTGSPATPPTGARGNYAAAIDENENTVWSTLQRDTATAAAFPQ
                     DITIDMGRQQQIKAFTYLPRQDHRLAGTADRYIFYTSADGDSWQKAAEGEFANIRANP
                     LEQLVPLDHPRTARYFKFSILHVVEGNGVTVAEVGVR"
BASE COUNT          628 a          973 c         1311 g          895 t
ORIGIN      
        1 atggataacc aggtttacta ctcccattat ttcttcaacc tgatctccaa tttcaccttc
       61 tattatttct acccggcgac gggtgatgag ttccgccagc acgagcggcg cgatctctat
      121 ggttatgatg ggaagataac gcataccgcc gggctgggcg ccggctcgct gacgtcgtcg
      181 gccggctggg gggtacgcta cgacgatgtc tccccctctt ttctcgccca cacgctgaat
      241 ggcgatagcc tccttaacta tatccaattg gggcagatgc gggaaaccgc tatgtatgga
      301 tccctcgatg agacgctgcg atcgggtaaa tggctggtcg atgccggggt gcgattggat
      361 tacctacatt tttattacgg gaatatggcg ccggctacgg ataccagcgc ggcgatctat
      421 gctggtgtca gtcccagggc ggggagggcg atcgtcagtc ccaagataac catacaatac
      481 tcggccaacc cgcgggttca gctgtatgta aaactgggga agggttttca ttccaatgat
      541 gcgcggatcg ttgtggcccg gcagggctac gagatcctcc cggctgccta tggcgccgac
      601 cttgggctca actggaaacc gctgccccgg ctttttgtca atgcggcgct gtggtggctg
      661 tacctgcaac aggagtttag gtatggcgcg gaccttggcg accaggctgt ttcccccggg
      721 ggccggacga ggaggaccgg catcgacttc tcggcccgat accagttcaa cgactggctg
      781 tttggcaacc tgaatgtcga cctggccagg ccccgtgacc tggatgcgaa gatgggtgaa
      841 aatttcctac cgctggcgcc gggcttcacc agtacggcag ccctggattt ccgtcttgcc
      901 agcggctgga acggcgggat cagttatcgt tatctgcgta accgtcccgc caatgccggc
      961 aatacgctta ctgctcctgg ttatttcgtg tcggatggca gtgtcaatta tacccggaag
     1021 cgatgggaga tggggctgac gatcgagaat ctgttcaacc agcgatggaa cgagtcgcag
     1081 ttcgattatg tttcccggtt gaaagatgag cggacgccgg ttgacgaggt gagttatacg
     1141 ccgggtgctc ctttcttcgc ccggctgaag tttacggtgt ttttttgaga cttgcggata
     1201 accgtgccct tctgagatct gcggttaagc ggaaagattt gtttttgggt ggagatcgat
     1261 ctattcccag gacggtatgg gcttgcccgt gtgccggtta ttcccaggac aacatgatct
     1321 tgccggtatg ccggccggct tccatttcgc ggtgggcttc ggcggcctcc gtatagggga
     1381 aggttcggta gatgaccggt ttgtaacggc ccgcggcgat gatgggccat actttttcct
     1441 ttatttcggt ggagagggct tttcgccagg cgtagtcgcg gacgcggagg gtgctgccgg
     1501 tgatggtcag ccgcctggcc atcatctggt ggaggttgag gtcgaccgtg ttgccggcca
     1561 ctgcatttat atagacgagg cggccttctt cgcggaggat gcggatgttc ttcttccaat
     1621 attcgccgcc gaccatgtcc aggatgacgt cgatgccgat gtcggcgagt tcgacggcga
     1681 agtcctgggt tttataattt ataaagcgat gggcgccgag gctgaggcag gcgttgccct
     1741 tttcttccga gccgacggtt acatagaccg tcgcgccgaa ggccttgccg agttggatgg
     1801 cggcggtgcc gatgccgctg ttaccgccgt ggatgaggag gttctcgccg gctgtcaacc
     1861 ggccgcgctg gaagatgttg gaccagctgg tgccgatggc ctcgggtagt gcggcggctt
     1921 caatgaagtc caggttagcg gggatgggga ggcattggcc ttctctcgcc gtcacgaatt
     1981 gtgcataccc gccgccggcg atgagggcgc agaccttgtc gccttgtttg aactgggtga
     2041 cggccggtgc gcagcgggtg atgatacccg atacttccag gccggggata tcggcgggga
     2101 cgccgggggg ggcgggatag ttaccgcccc gctggaagag atccgcccgg ttgaggccgg
     2161 aggcttttac ttcgatgagt acttcgtcgc cggcgggttg tggcgtctcg cgatcctgca
     2221 cctgaaggac gtcggggccg ccgggatgag ttatgatgat tgctttcatg cgctatgcgt
     2281 attgacccgg caaagctatg ccatattgtt gaaggccggc cttatcaccg tcgccggtct
     2341 tatcaccgaa cgccgacctc ggcgaccgtt actccatttc cttcgacgac atggaggatc
     2401 gagaatttga aataacgcgc ggtccgggga tggtccaggg gcacgagttg ttcgaggggg
     2461 ttggcgcgga tgttggcgaa ttccccttcg gcggcttttt gccaggagtc gccgtcggcg
     2521 ctggtgtaga agatataccg gtcggcggtg cctgcgaggc ggtgatcctg acgcggcaga
     2581 taggtgaagg cttttatttg ttgttgccgg cccatgtcga tggtgatgtc ctgcgggaaa
     2641 gcggctgcgg ttgccgtgtc ccgttgcagg gtgctccata cggtgttctc gttctcgtcg
     2701 atggctgccg catagttgcc gcgggcgccg gtggggggtg tggcagggga gccggttgtc
     2761 ccggctgtcg tggctgggaa gacaatcttc cactcccctt ttgagaggcc gaattctttt
     2821 gtcgttgtct cgctgtgggc gtcggccgat tcgaagctgc gggctttgac ggcgccgtcg
     2881 cctttttgtt cgaaaggaga ctgataggcc ggggattgca gggtgggttc gcttccgtcg
     2941 agggtataat ggatgctgcc gacggggccg gaggtagtta tgccgaccat tcccgccttg
     3001 tcgcgggtga tggtgggggg aacgaggtgg gtgggttctt tatataatcc gaaatcgctg
     3061 agggcgatgc atacgggagc ggagacgatg cggaggcgca gcttgcttgt ggtgacgttc
     3121 tgttgcaggc ggatcaggcg gcaggcgccg atactggtgc ctgcggctat gagcttccaa
     3181 tcgttgttct cccaggtgtc gacggcgaag gagtcgatgc gctggccgag ctttatgttc
     3241 tcgcggaggc ggatgacgtt gaagacggtg ggtttgccga gagtgatagt gagctgtggg
     3301 gtggtgaggt tgtcatcggt ggcccaatag gtgtagcgat cgccgtcgac caggcggccc
     3361 ggtccgtata gggagcggtt gccgccgcgg atattgctgg ccgtgaaggt ggcgtctttt
     3421 gcgaggttga ccgtaaagat ctgtttgagg agggcgccga attgttggag ggcggcgacg
     3481 tcgtgggagt cgagcaggcc gtcgcggttg ggagccaggc ccaggtcgag gccggcgccg
     3541 cggccgacgc tttggtagta aaggtcgagg agctggtatg gggttttgac ctgggcgtcc
     3601 tgtgacgcgt ggtagaacca gccgggccgc agggggacat cgcattccgc gggcatccag
     3661 aactccccgt tgcgggtgcc gctggtcgcc tgccaatatt tgctatagcc gttggagggt
     3721 ttcttccccg ggtcgggcgc ctcgggggta aaggtttccc agcaagtggt tcccgcatgg
     3781 ccttcttcgt tgccaaccca gcggacg
//
LOCUS       DATGFG010000885         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_28143, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000885 DATGFG010000000
VERSION     DATGFG010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28143"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VKQ52_16710"
     CDS             complement(<1..909)
                     /locus_tag="VKQ52_16710"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HLZ88900.1"
                     /translation="MNTGQLSEQELIRRDKLVELTGLGIDPFPAPLYPVNTTAGFIKE
                     KYKEGQNNADFADVCVAGRLMSRRDMGKASFASLQDSTGKIQIYIKRDEICPGEDKTF
                     YDKVWKHLLDLGDIVGVKGYVFTTKTGEISIHAKEFTVLTKALKPLPVAKEKEGETFD
                     AVTDPEFRYRQRYADLVINPQVKDVFVRRTKMINAMRSFLNERGALEVDTPVLQSIPG
                     GAAARPFITHHNALDVPFYLRIANELYLKRLIVGGFDWVYEFSRNFRNEGMDRTHNPE
                     FTVLEFYVAYKDYGWMMETTEQLLERA"
     gene            complement(966..1850)
                     /locus_tag="VKQ52_16715"
     CDS             complement(966..1850)
                     /locus_tag="VKQ52_16715"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLZ88901.1"
                     /translation="MTLDLVALSPVERQHFLQAAIAPRPICFASTIDAEGRVNLSPFS
                     FFNLFSTNPPILIFSPSRRVRDNTTKHSWHNVLEIPEVVINIVDYPMVQQASLASCDY
                     PKEVNEFVKAGFTAEPATLVRPPMVKESKVKIECRVLETKVLGETGGAGNLVICEALR
                     MHIDPAILDDEKKHIDQRRLQLVARLGGDWYCRVTPENLFAVEKPNTRLGIGMDALPD
                     PIRHSKTLSGNHLGQLANVHELPSVDPSFEDERLRSIFQYYSVSPDEMEKELHTYAAQ
                     LLDAGKVKEAWQVLLALS"
     gene            complement(1847..2863)
                     /locus_tag="VKQ52_16720"
     CDS             complement(1847..2863)
                     /locus_tag="VKQ52_16720"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLZ88902.1"
                     /translation="MKLVSYLKEEHEQLAVLVEGLLYDVELIHPDIPNTMNMFLNYWD
                     DLFPIMQAGVVAIEEGTRSSNKAIPVSSASLLAPVPLPASCRDGYAFRQHVAAARRNR
                     KVPMIPEFDQYPVFYFTNHHSIQGPGDVRCMPDHFEKLDFELEVAIVIGRFGRNIPAA
                     EADDYIAGLMIMNDLSARTLQLEEMLLNLGPAKGKDFATVTGPWLVTLDELQEFETAC
                     KEGHTGKSWDLRMQCRINGVQVSDGNLADMDWTFAELIERASYGVDLYPGDVIGSGTV
                     GTGCFLELNGTGKLNDPGYQEQWLKEGDEVELEVDGLGILGNTIVREDSDLSLLALKK
                     NRPS"
     gene            2907..>4147
                     /locus_tag="VKQ52_16725"
     CDS             2907..>4147
                     /locus_tag="VKQ52_16725"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLZ88903.1"
                     /translation="MHRFVLYCLLFVTGDCAFAQPRTDSFLVDLLRTHASPALLRVLD
                     KPDSFHYQLIYTRIDRDGHNRPHFTHFYYDVDAGDYFNPASTVKMPLAFLALEKMDSL
                     RRYGVDKETPMLTDSAYSGQTSAHTDSSSADGLPSLAQYIRKIFLVSDNDAYNRLYEF
                     LGQEAINRRLRQLGYPDVRITRRFVPMSEDENRHTNPIRFVRDGQTFYRQEAAVSHYP
                     FDFSRPVFYGNAYYDRHDSLIHAPMDFTRHNNFPLADAQQILQSVLFPGSVPADRRFR
                     LSPEDYLFLYQYMCELPRESVHPRYDTGEYFDSYTKFFLFKAGRTPIPPYIRIFNKPG
                     WSYGFLTDIAYIVDFEHKVEFMLTGTIYTNSDGVLNDDKYDYEEIGYPFFKEVGEIVY
                     RYELSRFRKVTPDLRAFRIFVP"
BASE COUNT          803 a         1160 c         1186 g          998 t
ORIGIN      
        1 ggccctttcc agcaactgtt cggtggtttc catcatccac ccgtaatcct tgtaggcaac
       61 atagaattcg agcaccgtga attccgggtt atgggtgcga tccatgccct cgttacgaaa
      121 gttgcggctg aattcgtaga cccagtcgaa cccgccgacg atcagccgtt tgagataaag
      181 ttcgttggcg atacggagat agaaggggac gtcgagggca ttgtggtggg tgataaaggg
      241 ccttgcggcg gcgccgccgg gaatgctttg cagcacgggg gtatctactt cgagggcgcc
      301 gcgttcgttc aggaagctgc gcatggcgtt gatcatcttt gtccgccgga caaagacatc
      361 ctttacctgg gggttgatga ccaggtcggc ataacgctga cgatagcgga actccgggtc
      421 ggttaccgca tcgaaggttt cgccttcttt ctcctttgcc accggcaagg gtttcaatgc
      481 ttttgtcagg acggtgaact cttttgcatg gatggagatc tcgcccgtct tggtcgtaaa
      541 gacatagcct tttaccccaa cgatgtcccc caggtcgagg aggtgtttcc agaccttgtc
      601 gtagaaggtc ttgtcttcgc cggggcagat ctcgtcgcgt ttgatataga tctggatctt
      661 tcccgtgctg tcctggagcg atgcgaagga ggccttaccc atgtcccggc ggctcatcag
      721 gcggcccgcg acacagacat cggcaaagtc cgcgttattt tgcccttcct tatacttctc
      781 cttgataaaa cctgcggtag tattgacggg atataatggc gcggggaagg gatcgatgcc
      841 cagcccggtc agctctacta atttgtcccg gcggatcagt tcctgttctg ataattgtcc
      901 tgtgttcata cgtaaaagtt cggcgaagat agatcattcg gcttgaggcg ccaagggagg
      961 gagggtcagc tcagggccag gaggacctgc caggcttctt ttaccttgcc ggcgtccagc
     1021 aactgcgccg cgtaggtatg gagctctttt tccatttcat ccgggctgac ggaatagtac
     1081 tggaagatac tccgcagccg ctcatcctcg aaggaggggt ccacggaagg cagctcatgg
     1141 acattggcca gctgtcccag gtggttgccg ctgagggtct tgctgtggcg gatagggtcg
     1201 gggagggcgt ccatcccgat gccaagccgg gtattgggtt tttccacggc gaaaagattc
     1261 tcgggtgtga cccggcaata ccagtcaccg cccagccgcg ccacgagttg cagccggcgc
     1321 tggtcgatat gtttcttttc gtcgtcgagg atggcgggat cgatatgcat ccgcagcgct
     1381 tcgcagatga ccaggttgcc ggcgcctccg gtctcgccca ggacctttgt ctccagcacg
     1441 cggcattcga tctttacctt gctttccttc accatcggcg gccgcaccag ggtagccggc
     1501 tccgcggtaa agcccgcctt gacgaactcg ttcacctcct tcgggtagtc gcagctggct
     1561 aggctggcct gctgtaccat cgggtagtcg acgatattga tcaccacttc ggggatctcg
     1621 agcacattgt gccagctgtg tttggtggta ttgtcgcgca cccgccgcga gggcgaaaag
     1681 atcaggatcg gcgggttggt ggagaacagg ttgaagaagc taaaggggct gagattgaca
     1741 cggccttccg cgtcgatggt gctcgcgaaa caaatgggcc tcggggcgat ggcggcctgc
     1801 aggaaatgct gtctttcgac ggggcttagc gcaacgaggt cgagggtcat gacgggcggt
     1861 tttttttcag cgcgagaagg gagagatcgc tgtcttcccg gacgatggta ttgcccagga
     1921 tgcccaggcc atcgacttcc agttccacct cgtccccctc ctttagccat tgctcctggt
     1981 aaccgggatc gtttagcttt cccgtgccgt tgagctccag gaaacagccg gtccctaccg
     2041 ttccgctgcc gatcacatct ccgggataaa gatcgacgcc ataggaagcc ctttcgatga
     2101 gctcggcaaa ggtccagtcc atatccgcca ggttgccatc gctcacctgg acgccgttga
     2161 tccggcattg catccgcaga tcccagctct tgcctgtatg gccttccttg caggcggttt
     2221 cgaattcctg cagttcgtcg agggtcacga gccagggacc ggtcaccgtt gcaaaatcct
     2281 tgcccttggc gggtccgagg ttgagcagca tttcttccag ctgcagggtc cgggcgctca
     2341 ggtcgttcat gatcatcaat ccagcgatat agtcgtcggc ttcggcggcc gggatattac
     2401 ggccaaagcg gccgatcacg atcgctactt ccagctcgaa gtccagcttt tcaaaatggt
     2461 ccggcatgca gcggacatcg ccggggccct ggatgctatg gtggttggta aaataaaaga
     2521 cgggatactg gtcgaattcg gggatcatcg gcactttgcg gttgcgtctt gcggcggcca
     2581 cgtgctgccg gaaggcatag ccatcgcggc aggaggccgg taggggcacc ggcgccagca
     2641 gcgaagcgga gcttaccggt atggccttgt tcgaactccg cgtaccttct tcgatggcga
     2701 ccacgcccgc ctgcatgatc gggaagagat cgtcccagta gttgagaaac atattcatcg
     2761 tattgggaat atcgggatgg atcagctcca cgtcatagag gaggccctcc accagaacag
     2821 ccagctgttc atgttcttct ttcaagtagg agacaagctt catggtcgcc aaaggtattg
     2881 gatttaaagg attatttttg atctctatgc accggtttgt cctttattgc ctgctctttg
     2941 taacgggtga ttgcgccttc gcccaacccc ggaccgacag cttccttgtc gacctcctgc
     3001 ggactcatgc ctctccggcc ctgctccggg ttctcgacaa gccggactcc tttcattacc
     3061 agctcatcta tacccggatc gatcgcgacg gtcataaccg gccccatttt actcattttt
     3121 attacgatgt ggatgcgggg gactatttta accccgcttc cactgtgaag atgccgcttg
     3181 cctttctcgc cctggagaaa atggatagcc tgcgtcgcta tggcgtcgac aaggagaccc
     3241 ccatgctgac ggatagcgcg tatagcggcc agaccagcgc gcatacagat agcagttcgg
     3301 cggatggtct tccgtccctg gcgcagtata tccggaagat cttcctggtc agcgacaacg
     3361 acgcgtataa ccggctatac gagttccttg gacaggaggc gatcaaccgc cgtctccggc
     3421 agctgggcta tcccgatgtc cggatcacgc gccgttttgt gccgatgagc gaggacgaga
     3481 accgccatac caacccgatc aggtttgtcc gcgacggtca aaccttctat cgccaggaag
     3541 ccgccgtgag tcattacccc ttcgatttca gccggccggt tttttatggc aatgcctatt
     3601 acgatcgcca tgactcgctg atccatgcgc ccatggattt tacccggcac aataatttcc
     3661 cactggcgga tgcgcagcaa atactgcagt cggtgctctt tccgggatcg gtgccggcgg
     3721 accgccgttt ccggctcagc ccggaggatt accttttcct gtaccagtat atgtgcgagc
     3781 tgccgcggga gtcggtccat ccccggtatg acacgggcga atatttcgat agctatacca
     3841 aattcttttt gttcaaggcg ggccgcaccc cgatcccacc ctatatccgc atttttaaca
     3901 aacccggctg gtcctatggc ttcctgacgg atatcgccta tatcgtcgat tttgaacaca
     3961 aagtggagtt tatgcttacg ggtacgatct ataccaacag cgatggggtg ttgaatgacg
     4021 ataaatatga ctatgaggag atcggctatc ctttctttaa agaggtgggg gagatcgtct
     4081 accggtatga gctttcccgt ttccggaaag ttacccccga tctccgggct tttcgtattt
     4141 ttgtacc
//
LOCUS       DATGFG010000886         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_732723, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000886 DATGFG010000000
VERSION     DATGFG010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732723"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3020
                     /locus_tag="VKQ52_16730"
     CDS             <1..3020
                     /locus_tag="VKQ52_16730"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLZ88904.1"
                     /translation="SVLLREQGVGPHTIAAVLLQRSPELIIGILAVLMAGGCYLPIEP
                     GLPDERIGLLLGQSRCGLVITEDSYISRLSVAGAYGVTVIDVNRTDLWAPRPGMMEDF
                     GCADDPAYVIYTSGTTGRPKGVMIAHRSLVNYIWWAAATYIKEPEAVFPLYTSVAFDL
                     TVTSIFTPLITGNKIVVYNEEERDLLIRQVVIDNKCTIVKLTPSHLKILAAGKIPAES
                     RIKRFIVGGEQLETSLAQEIYDRYSGQVEICNEYGPTEATVGCMIHSFVPGETAGNVP
                     IGVAAANMRVYILDKYLKPVAAGVHGELYVSGDGIAMGYLFMEELTKEKFVSDPFSEG
                     RRMYRTGDIARWLPEGVMEYLGRVDEQVKINGYRIEPAEIVHCLRSHPRIAEAVVTIK
                     AGTGGTQFLCAHIKLKEQTDRIDAEAELRSYMAGKLPYYMIPASFVVIDEIPLTKNGK
                     IDHSALPAPVAGDGRRAIYPRSELENIVTKVWEEVLEQPALSVNDNFFAFGGDSIKAV
                     QIASRLLDKGIPVHAKDILIYHTIEQLCLHTDLAAVGKVYEQGPVEGSKGLIPIESWF
                     FAQQFTNPDFFTQSILLGLNKTIDKGLLERAFEALIRHHDGLRLNCDPGREVLFYHAD
                     FLRSPFVIGSYETGDGSGDDDLAGICREIKNGFCITDGLLLRSAIIKDAAMGQFLFIT
                     AHHLVVDGMSWRILLEDLYTLYTDLEKGIHTRLPRKTASLTAWRQKWEEFARQHDTEE
                     VRKYWEEIEKTRFSIPLDADTEDWEARNREEVSGLLNRNVTTALLKEAYSAYKSDTLV
                     FLSTALALTLRAWTQSEVLVVEYEGHGRHPELIDTSRTIGWFTEMYPVRFELPGGNAE
                     NELKAIKNQLRNATDRGAEYGVTNYFKAKKGGTALTEIRFNYLGQFSTELDNDLFTYS
                     FRSTGSDIDPRNRMTAKLELVAMIINGELRVQLYYNRRAHTRQTVRSLIDDFFNNLNR
                     IVSHIKEKNDVFLTASDFDAAGLDDEELNVLFS"
     gene            3164..>4159
                     /locus_tag="VKQ52_16735"
     CDS             3164..>4159
                     /locus_tag="VKQ52_16735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88905.1"
                     /translation="MLRHHLIIIFRTFKRNKTMFLINLLGLSVGLACSLMIYLWVHDE
                     LNVDRFHEKIDRLYEVMVNQKESNGIVTDVNGPALLAETLAHDYPEVKYAANTTGVFQ
                     LIVSVNNNHFEPRVQFASKDYFNVFSYELISGDKNQVLANRNAVVLSASLARKLFGTT
                     ENVIGKPVKWQFLTLGEDCIVTGVFKDVPANSSEQFDCAFSFEAYKAIEHNSYWGNLN
                     AKTYVVLDKGVNPDQFSQKIAGEVKKHLAESNVTLFIRPYSSMYLYGKFENGAAAGGR
                     IEYVKLFSSIAIFILVIACINFMNLSTARAAKRLKEVGIKKVVGAHRWSLVFQYLS"
BASE COUNT         1083 a          898 c         1146 g         1032 t
ORIGIN      
        1 tatcggttct tttgagggaa cagggagttg gtccccatac tattgcggct gttctattgc
       61 agcgatcgcc tgagctgatc atcggtatcc tggctgtttt gatggccggc gggtgttatt
      121 tacccatcga gccagggcta cccgatgagc gtatcggttt gttactcggg caaagccgat
      181 gcgggcttgt gatcactgag gatagctata tcagccggct gtccgtcgcc ggggcgtatg
      241 gggtgacggt gatcgatgtg aacagaacgg acctttgggc gccacggccg ggtatgatgg
      301 aagactttgg ctgtgctgat gatcctgctt atgttatcta tacatccggc acaacggggc
      361 ggccaaaggg ggtaatgatc gctcaccgga gccttgtcaa ttacatatgg tgggctgcgg
      421 ccacctacat taaggagccg gaagcggttt ttcccttgta tacttctgtc gccttcgatc
      481 ttaccgtcac atctattttt actcccttaa ttaccggcaa taagattgtg gtatataatg
      541 aggaagaaag ggatctgctg atcaggcagg tagttatcga caataaatgt acgatcgtca
      601 agctgacgcc atcccatctt aaaatactgg ctgccggcaa aatacccgcg gaaagcagga
      661 taaaacggtt tatcgtcgga ggggagcaat tggaaacctc gttggcccag gagatatatg
      721 accggtattc ggggcaggtg gaaatatgca atgaatatgg gcctacggaa gcgaccgttg
      781 gttgcatgat ccattctttt gtaccgggcg agaccgcggg gaatgtgccg atcggcgtcg
      841 ccgcggccaa catgcgggtg tatatcctgg ataagtacct aaaaccggtg gccgccggcg
      901 ttcatgggga attgtatgtg tcgggtgacg gcattgccat gggatacctg tttatggaag
      961 agttgacgaa ggagaaattt gtttctgatc cttttagtga aggccggaga atgtacagga
     1021 cgggtgatat cgccagatgg ttacccgaag gcgtcatgga atacctggga agggtcgatg
     1081 agcaggtgaa aataaacggt taccggatcg aaccggccga gatcgtccat tgtctgagga
     1141 gtcatcccag gatagcagag gcggttgtta ctattaaggc gggtaccggg ggaacgcaat
     1201 ttttgtgtgc ccatatcaaa ctgaaggagc aaacagatcg tatcgatgct gaggcggaat
     1261 taagaagcta tatggcgggc aaattgccct actatatgat ccccgccagt tttgttgtga
     1321 tcgatgagat cccgttaacg aagaatggca aaatagacca cagtgccttg cccgcaccgg
     1381 tggccggcga cggcagacgg gcaatatatc ccaggagcga gttagagaat atcgttacga
     1441 aggtgtggga agaggttttg gaacagccgg cattgtccgt caacgataat ttttttgcat
     1501 ttggtggcga ctccatcaag gcggtgcaaa tcgcctcgag gttgctcgat aaagggatac
     1561 cggttcatgc aaaagacata cttatatacc ataccatcga gcagctatgt ttacataccg
     1621 acctggcagc tgtcggaaaa gtgtatgagc aggggcccgt tgaagggagc aaggggttaa
     1681 tacccattga aagctggttt tttgcgcagc aattcaccaa cccggatttc tttactcaat
     1741 ctattttgct aggactcaac aaaacgatcg acaagggtct gctcgaaagg gcattcgaag
     1801 cgctgatcag gcatcatgac gggttgcgat tgaattgcga ccccggaagg gaggtattat
     1861 tctaccacgc agactttctc cgatcccctt ttgtgatcgg gtcttatgaa accggagatg
     1921 gcagcggcga cgacgatctg gccggcatct gccgggaaat aaagaatgga ttctgcatca
     1981 cagatggtct tttgctgaga tcagctataa taaaggatgc agcaatgggg cagttcttat
     2041 ttattacggc gcatcacctg gtggtggatg ggatgtcatg gagaattttg ttagaagacc
     2101 tgtatacgtt gtatacggac ctcgaaaagg gtatccatac gaggcttccc cgtaaaacgg
     2161 cctcgttgac cgcctggcgg caaaaatggg aagagtttgc acggcaacat gatacggaag
     2221 aagtaagaaa atactgggaa gaaatagaaa aaacccgttt ttccatcccc ctggatgccg
     2281 acaccgaaga ttgggaggcc cgtaaccggg aggaggtctc agggctgcta aacaggaatg
     2341 taactacggc cctgttaaag gaagcctatt cagcctataa aagcgatacc cttgtatttt
     2401 taagcacggc gcttgcgctt acgctcaggg cgtggacaca aagtgaggtt ttagtagttg
     2461 aatatgaagg ccatgggcgg catccggaat tgatcgacac ctcccgtacg ataggatggt
     2521 ttacggaaat gtatcccgta cgattcgagt tgcccggcgg caacgcagaa aatgaattga
     2581 aagctataaa gaaccaattg aggaatgcga ccgatcgggg ggcggaatat ggggtgacca
     2641 actatttcaa ggcaaagaaa ggcggcacag ccctgacgga aataagattc aactatctgg
     2701 gtcagttctc caccgagctg gacaatgatc tgtttactta cagctttcgg tccaccggct
     2761 ccgacatcga tccccgcaac cggatgacgg caaaactgga gcttgttgcc atgatcatca
     2821 acggggagct gcgggtgcag ctttactata atcgcagggc acacacccgg caaacggtgc
     2881 ggtcactgat cgatgatttt ttcaacaatt tgaaccggat cgtcagtcat ataaaagaaa
     2941 aaaatgatgt atttttgact gcatccgatt tcgatgccgc aggcctggac gatgaagagt
     3001 tgaatgtgtt gtttagttag tgtacgaaaa cgaacaaggg gtgttcgaaa atgaacagtt
     3061 gcgggattgg aaatcaagcg gccaggggag gaaccccctt ggccggactt atggcacttt
     3121 tattgtggtt gcacccccca gagccattat caattagtat attatgcttc gtcaccacct
     3181 tatcatcatc tttcggactt tcaagcggaa taagaccatg tttttgatca acctgttggg
     3241 gttatccgtt ggattagcct gttccttaat gatctatcta tgggtgcatg atgaactgaa
     3301 cgtcgacagg ttccatgaaa agatcgatcg tctttacgag gtgatggtaa atcaaaagga
     3361 atcgaatggt atcgtcacgg atgtcaacgg ccccgcctta ctggccgaaa cgctggcgca
     3421 tgattatccc gaagtaaaat atgcagcaaa tactacgggg gtcttccaac tcatcgtatc
     3481 cgtaaataac aatcatttcg agccccgggt gcaattcgcg agtaaggatt atttcaatgt
     3541 tttttcctat gagctgatct cgggagataa gaaccaggta ttggccaacc ggaatgcggt
     3601 tgttctttcg gcaagcctgg ccaggaagtt attcggcacg acggaaaatg tgatcggcaa
     3661 gcccgtaaaa tggcagttcc ttacgctggg cgaggattgt atcgttaccg gcgtttttaa
     3721 ggatgtgcct gccaattcct cggaacaatt cgattgtgca ttctccttcg aagcgtataa
     3781 agcaatagaa cataatagtt actggggtaa tcttaatgcc aagacctatg ttgtcctgga
     3841 taaaggggtc aatccggatc agttcagcca aaagatcgcc ggagaggtta aaaagcattt
     3901 ggcggaatcc aatgtaacct tatttatacg cccctattca tccatgtatt tgtacggcaa
     3961 attcgagaat ggggcggccg ctggtggaag aatagagtac gtaaagctgt tttccagcat
     4021 tgctattttt atattggtca ttgcctgtat caatttcatg aacctttcta cggcgagggc
     4081 tgccaaaagg ctgaaggaag tgggtattaa aaaggtggtc ggcgcccatc gatggagcct
     4141 ggtgtttcaa tacctgtcc
//
LOCUS       DATGFG010000887         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_505338, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000887 DATGFG010000000
VERSION     DATGFG010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505338"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VKQ52_16740"
     CDS             <1..402
                     /locus_tag="VKQ52_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88906.1"
                     /translation="GKWPNKQLLQARLLSDGGYFTDALQLLQGTSSSDFTTAEEKCEY
                     AYRLGRIYDGLGRGDEAIEAYLTTIKTGEQLKEYYAARAALQIGYIYEQRGDKMRALA
                     FFEKCLSLKDHDYKNSLDQRAKAGVARCKGE"
     gene            448..2103
                     /gene="recN"
                     /locus_tag="VKQ52_16745"
     CDS             448..2103
                     /gene="recN"
                     /locus_tag="VKQ52_16745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HLZ88907.1"
                     /translation="MLQRIQIQNYAIIDALSIDLSQRLNVITGETGAGKSILMGALSL
                     ILGDRADTAVLRQRDKKCVVEGVFALGGKKEVAAFFAANDLDVDEEVVIRREIAPSGK
                     SRAFVNDTPVNLEQLRGLSSLLVDLHRQFDTLELGESDFQREVLDALAGQGNRVEDYR
                     GLYRSWQAAARELATLREQQAQFNKELDYNKFLFDELRELNLREKELEELDQELKILN
                     NSEGIKTALSKVYYELKEGEQPLLQQLKTLQHSLQAYAAYHPELSSLAERMSSAQIEL
                     QDIAGEVERINEEVGYDPRRIEEINERLSSGYKLLKKHGVQTTGALLEIYRQLEHKLQ
                     DSFQVDAGIAAGEKEVNRLLQDAKKAAAVLSKARQAQTGSLEEKVNKLLKQVGMPNAR
                     LKVRVLPVELNAFGADEVEFLFDANKSERYEPIRKVASGGELSRLMLCIKSLVAESID
                     LPTLIFDEIDTGISGEAARQVGIILKGLAKKRQVICITHQPQIAGKADAHYFVYKAVK
                     GEAIQTNIRLLSQDERITAIAQMLSGEKPTAAALENAREMVLG"
     gene            2108..2311
                     /locus_tag="VKQ52_16750"
     CDS             2108..2311
                     /locus_tag="VKQ52_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88908.1"
                     /translation="MKLRSLLILVGLLLAMVPVYYLNRWLQRVMRPRESAGRFFLFIF
                     TNFLLIVVYTVFIVGMVVRLFGR"
     gene            2345..3157
                     /locus_tag="VKQ52_16755"
     CDS             2345..3157
                     /locus_tag="VKQ52_16755"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ88909.1"
                     /translation="MAYNLLKGKKGIIFGALDEKSIAWRTALKCYEEGAQLVLTNAPV
                     ALRMGEINKLAEAVKAPVIGADVTNMDDLKKLFEESMKHFGGGIDFVLHSIGMSLNVR
                     KGKSYTEIDYEWNKKTLDISAMSLHRVLRTAWDLDALNEGASVIALTYIAAQRVFPDY
                     NEMADAKALLESVTRSFGYYYGVKKKVRVNTVSQSPTRTTAGSGVKGFDGFVSYAEKM
                     SPLGNASADQCADYCVTLFSDLTKMVTMQNLFHDGGFSYTGVTEAVINQMEK"
     gene            complement(3195..3416)
                     /locus_tag="VKQ52_16760"
     CDS             complement(3195..3416)
                     /locus_tag="VKQ52_16760"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HLZ88910.1"
                     /translation="MFWTLELASYLEDAPWPATKDELIDYAIRSGAPIEVVENLQELE
                     DEGEIYEGIDDIWPDYPSQEDFFFNEDEY"
BASE COUNT          808 a          729 c         1190 g          862 t
ORIGIN      
        1 gggaaatggc ccaacaaaca actgttgcag gcgcgattgc tgagtgatgg gggatatttt
       61 acggatgccc tgcagctttt acaggggacg agcagctccg attttacgac ggcggaagag
      121 aagtgcgagt atgcctaccg gctggggcgt atctatgatg ggcttggccg gggggatgag
      181 gctatcgaag cttatctgac cacgatcaag acgggggagc aactgaaaga atattatgcg
      241 gcgagggcgg cgttgcagat cggctatatt tatgaacaac gtggggataa aatgcgggcg
      301 ctggcgtttt tcgagaagtg cctttccctt aaggatcatg actataagaa ttcgcttgac
      361 cagcgggcga aggcgggtgt cgcgagatgc aagggtgaat agcgggaaat ttccttaatt
      421 tgccagccgt taatttggct gttgtttatg ctacagagga tccagataca gaactatgcg
      481 atcatcgatg cgttgagcat cgatctttcc cagcggttaa atgtgatcac gggagagacg
      541 ggggcgggta aatccatcct gatgggtgct ctttcgctga tcctggggga tcgtgccgat
      601 acggcggtgt tgcgtcaacg ggataagaaa tgtgtggtag agggcgtttt tgcgcttggc
      661 gggaagaagg aggtggcggc tttttttgcc gccaatgacc tggatgtgga tgaggaggtt
      721 gtcattcgcc gggagatcgc accttcgggg aaatcccggg cctttgtgaa tgatacgccg
      781 gtgaaccttg agcagttgag ggggttgagc tctttgctgg tggacctgca ccggcagttc
      841 gatacgctcg agctcggcga gtcggatttc cagcgggagg tgttggacgc gctggcgggt
      901 caggggaatc gggtggagga ttatcgcggc ctctatcgca gctggcaggc cgcggcccgg
      961 gaactggcga ccctgcggga gcaacaggcg caattcaaca aggagcttga ctataataaa
     1021 tttctttttg acgagctgag ggagttgaat ctgcgggaga aggaattgga ggagctggac
     1081 caggagctaa agatcctgaa caactcggag gggatcaaga cggcactttc caaagtgtat
     1141 tacgaactga aagagggaga gcaacccttg ctgcaacagt taaagacgct gcagcattcc
     1201 ctgcaggcct atgcggcgta tcatccggaa ttgtcttcgc tggcggagcg gatgtcgtcg
     1261 gcgcagatag aattgcagga cattgccgga gaggtagagc ggataaatga ggaggtgggg
     1321 tatgatccac gccggatcga ggagatcaac gagcggcttt cctcggggta taaattattg
     1381 aagaaacatg gggtgcagac gacgggcgcg ctgctggaga tctatcggca gttggagcat
     1441 aagctgcagg attccttcca ggtggatgcg ggtattgcgg ccggggagaa ggaagtgaac
     1501 cggttgttac aggacgcgaa gaaggcggcg gcggtgctgt ccaaggcccg gcaggctcag
     1561 acggggtcat tggaggagaa ggtgaacaag ctgctgaagc aggtggggat gcccaatgcc
     1621 cggctgaagg tgcgggtgtt gcccgtcgag ctcaacgctt ttggggcgga cgaggtggaa
     1681 ttcctttttg acgccaacaa gagcgagcga tatgagccga tccgtaaggt agcttccggt
     1741 ggcgaattga gcaggctgat gctttgtata aaatcccttg tggcggagtc catcgatctg
     1801 ccgacgctta tttttgacga gatcgatacg gggatctccg gtgaggcggc aaggcaggta
     1861 gggatcattt tgaagggatt ggcgaagaaa cggcaggtga tctgtatcac gcatcagccg
     1921 cagatcgccg ggaaggcgga tgcgcattat tttgtataca aggccgtcaa gggagaggct
     1981 attcagacca atatccgttt gctgagccag gatgagcgga tcaccgcgat cgcccagatg
     2041 ttgagcgggg agaagcctac ggcggcggca ttggagaatg cccgggagat ggtattggga
     2101 tgagcatatg aaacttcgtt ctttactcat attggtgggt cttctgctgg cgatggtgcc
     2161 ggtgtactac ctcaaccgct ggctgcagcg ggtgatgcgt ccccgtgaat cggcggggcg
     2221 gttcttcctt tttatattta cgaatttttt gctgatcgtg gtgtataccg tttttatcgt
     2281 ggggatggtg gtgcggttgt ttgggcgata attgtatata tttggcgtct taaaaattca
     2341 atctatggca tacaacctgc ttaaaggtaa aaaagggatc atattcgggg ctttggatga
     2401 aaagtccatt gcgtggcgga cggctttgaa atgctatgag gaaggtgcac agcttgtgct
     2461 tacgaatgcg cctgtggcgt tgcggatggg ggagatcaat aagttggccg aggcggtgaa
     2521 ggctccggtg atcggtgcgg atgtgaccaa tatggatgat ctgaagaaat tattcgagga
     2581 gtcgatgaaa cattttggcg ggggtatcga ttttgtcctg cattccattg ggatgagcct
     2641 gaatgtgagg aaggggaaga gctatacgga gatcgattat gagtggaaca agaagacgct
     2701 ggatatttcg gcgatgtccc tgcaccgggt gttgcggacg gcctgggatt tggatgcgct
     2761 gaacgaaggg gccagtgtga tcgcgctgac ctatatcgct gcgcagcggg tgttcccgga
     2821 ttataatgag atggcggatg ccaaggcgtt gctggagagt gtgacgcgga gttttggtta
     2881 ttattatggg gtgaagaaga aggtgcgggt gaatacggtt tcgcagtcgc cgacgcggac
     2941 gacggcgggc agcggggtaa agggttttga tgggtttgtc agctatgcgg agaagatgag
     3001 cccgctgggg aatgcttcgg ccgatcaatg cgccgactat tgtgtcacgt tgttcagtga
     3061 tctgacgaag atggtgacga tgcagaattt atttcatgat ggggggttct cgtataccgg
     3121 ggttacggaa gcggtgatca accagatgga gaaataagta aaaagcgccc cgcggggcgc
     3181 tttttttatg ggttttagta ttcatcctcg ttgaaaaaga aatcttcctg gctgggatag
     3241 tcgggccaga tgtcgtcgat cccttcgtag atctcgcctt cgtcttccag ttcctgtagg
     3301 ttctctacca cttcgatggg ggcaccggag cgaatagcat agtcgatcaa ttcatctttg
     3361 gtagccggcc agggggcatc ttccaggtag ctggctaatt caagtgtcca aaacatcgct
     3421 cgtaattttt cgcaaaagta atttttaaaa tgatttctcc aaatttctcc tttcgaaatc
     3481 tccaattctg agtcctaacc tgtacgtttg tgctatgcgt ttacggtgcc aaaccgggtg
     3541 gagccccatt cgtactcgag gtacgcggga cctgcgtaca acgccgcga
//
LOCUS       DATGFG010000888         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_371479, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000888 DATGFG010000000
VERSION     DATGFG010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371479"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VKQ52_16765"
     CDS             <1..847
                     /locus_tag="VKQ52_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88911.1"
                     /translation="AILFSPDQPLETMQLARCDHFFRGAGRNSLRRILDILYEWDAYQ
                     AVAQAAQQYDLSFPHYSDAQTPAVEAQGLFHPLLTNAVTNDYQMGGAVNLCFISGANM
                     AGKSTFLKSMGLAVYLSHVGFPVPARFWQTTLFNGLITTVNLPDNISQGYSHFYSEVR
                     RVKEVVMKVKEKKKMVVIFDELFRGTNVKDASDASLRVIEGLAGVGGSLFLVSTHIVE
                     IAAGLQRNPHIFFACFESTMEGGVPWYSYTLRSGISNERMGMLILRNEGILEMLEEMK
                     GESPQ"
     gene            868..>3159
                     /locus_tag="VKQ52_16770"
     CDS             868..>3159
                     /locus_tag="VKQ52_16770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ88912.1"
                     /translation="MLKSMLLIAVRHLRKRKGYAVLNILGLTAGIACCLLIFEYVAYE
                     RSYERFNPKADRIVRVQNEEYQNGKMTVPCASSMPALAPALKKEIPEVEEACRIRHWP
                     LVLTNESRQVRFREGKVYFADPSVLDIFAISLLEGDARTALSAPGKILLSAATAKKYF
                     GGEDPLGKVLQIPGGGGKRPLEVTGVFRDYPANSHVRFDLLVSYKTYSQINGTYGQPD
                     DQVETSWGWTDYYTYVLLRQGADRQGFAAKMPALIDRHYNNLPENLANKDRFGLTVVP
                     LKDIHLYSHYTEEAEPNGDGQSVFFLFLIAFFIIGIAWVNYINLSTARSLERAREVGV
                     RKVLGALRRELIRQFMVESLLLNTVALLAAMALVVAVNPLFGHLTGRPLTVVFALPAA
                     YWGYFAVLFVAGTFLSGIYPALVLSRYQPVVVLKGLFKNAAGGQWLRKGLIVGQFAAS
                     IILIAGTMIVYRQVSYMRSQRLGADIDQTLVVRGGGAGFADSAYREAFDAFKREALQV
                     KGVKSMTASSNVMGEEILWSTDWWWMQNPGGTRKVETFMLGVDEDFVPAYGILMVAGR
                     NFTRDFPTDRRAVLLNESAVRSLGFPSSRAAVGQLVGTGWRGFDSAHIVGVIADFHNE
                     GLQKAIQPLVLLPHSDGRANYSLKVQVADAGATVAAIKAIWDRHFPGDPYNYFFLDEF
                     FSRQYAENQRFGEVFALFAVFAVVIACFGLLGLSANNVLQRTKEIGIRKVLGASVNSL
                     LYLLSRDFLVLVVVALVIAVPVTW"
BASE COUNT          602 a          742 c         1046 g          769 t
ORIGIN      
        1 ggccattctt ttttcccccg accagccgct ggagacgatg caactcgcgc gttgcgatca
       61 ttttttccgg ggtgcaggcc ggaactccct gcgccggata ctcgatatcc tgtatgagtg
      121 ggatgcgtac caggcggtgg cccaggcggc ccagcaatac gatctttcct tcccgcatta
      181 tagtgatgcg cagactcctg ccgtcgaggc gcagggcctt ttccatccct tgcttaccaa
      241 tgccgtcacc aacgattacc agatgggagg ggcggtgaac ctgtgtttta tcagcggggc
      301 caatatggcg gggaagtcca cttttctgaa atcgatgggg ctggcggtct atctctcgca
      361 tgtagggttc cctgttcccg cgcgtttttg gcagacgacg ctgttcaatg gattgatcac
      421 aaccgttaat ctccccgaca atatcagcca ggggtacagt catttttata gcgaggtccg
      481 gcgggtgaag gaggtggtga tgaaggtgaa ggagaagaag aagatggtgg tcatcttcga
      541 tgagctgttc cgggggacga acgtcaagga tgcttccgac gcgtcgctgc gggtgatcga
      601 agggctggcg ggtgtcggcg ggagcctgtt cctggtgtct acgcatatcg tggagatcgc
      661 ggcggggctg cagcggaacc cgcatatctt ttttgcctgt ttcgagtcta cgatggaggg
      721 cggggtgccg tggtacagtt ataccctccg gagcgggatc tccaatgagc ggatggggat
      781 gctgatcctg cggaacgagg gcatattgga gatgctggag gagatgaagg gggaaagtcc
      841 acaataacga taactcaaca cattcctatg ttaaaaagta tgctgctgat cgcggtccgt
      901 catctgcgta agcgtaaggg ctatgcggta ttgaatatcc ttggactcac ggcgggtatc
      961 gcctgttgtc tgctcatctt cgagtatgtt gcgtatgagc ggagttatga acgcttcaat
     1021 ccgaaggcag acaggatcgt aagagtacag aacgaggaat accagaatgg caaaatgacg
     1081 gttccctgcg cctcttcgat gccggcgctg gcccctgccc tgaaaaagga gatcccggaa
     1141 gtggaggagg catgccggat ccgtcattgg ccacttgtgc tgaccaatga aagccgtcag
     1201 gtaaggttcc gggaagggaa ggtctatttt gcggaccctt cggtattgga tatttttgct
     1261 atctccctgc tggagggcga tgcgaggacg gctttgtcgg cgccagggaa gatccttttg
     1321 tcggcggcca cggcaaagaa atactttggg ggagaagacc cgttgggaaa ggtattgcag
     1381 atccccggcg gtgggggcaa gcggccgctg gaggtgacgg gggtattccg ggattatccg
     1441 gccaactcgc atgtccggtt cgatctgctt gtttcctata aaacgtatag ccagataaac
     1501 gggacatatg gtcaaccgga cgaccaggtg gagaccagtt ggggctggac ggattactat
     1561 acctatgtat tgctgcggca gggcgccgac aggcaggggt tcgcggccaa aatgcccgcg
     1621 cttatcgacc ggcattataa taacctgccg gagaacctgg ccaataaaga ccggtttggc
     1681 cttaccgtcg tgccgctgaa ggatattcac ctgtattcgc actataccga agaggcggag
     1741 cctaacggag acgggcagtc ggtatttttc ttgttcctca tcgctttttt tatcatcgga
     1801 atcgcctggg tcaactatat caatctctct acggcgcggt cgctggagcg tgcccgggaa
     1861 gtgggggtgc gcaaggtgtt gggggcgttg cggcgggaac tgatccggca gttcatggtg
     1921 gagagcctgt tgctaaatac ggtggcgttg cttgcggcga tggcgttggt agtggcggtg
     1981 aatccgctgt tcgggcattt gacggggcgg ccgctgaccg ttgtgttcgc gctgccggcg
     2041 gcctattggg gatattttgc ggtgttattt gttgccggaa ccttcttgtc agggatctat
     2101 cccgcattgg tattgtcgcg ttatcagccc gtggtggtgc tgaaggggct tttcaaaaat
     2161 gcggcgggcg gtcaatggct acggaagggc ctgatcgtgg ggcagtttgc ggcttccatc
     2221 atcctgatcg cggggacgat gatcgtctac cgccaggtgt cgtatatgcg cagtcagcgg
     2281 ttgggagcgg atatcgacca gacccttgtc gtaagagggg gcggcgcggg attcgcggat
     2341 tctgcttacc gggaggcgtt cgatgcgttc aaacgggaag ccttgcaggt gaagggtgtg
     2401 aagagcatga cggcctcatc gaatgtaatg ggggaggaga tcctgtggtc taccgattgg
     2461 tggtggatgc aaaaccccgg tgggacacgg aaggtcgaaa cctttatgtt gggggtggat
     2521 gaggattttg tccctgccta tgggatcctg atggtagccg ggcggaattt cacccgggac
     2581 tttcccaccg atcgccgggc ggtattgctg aatgagtcgg ctgttcgcag cctgggattc
     2641 ccctcgtccc gggcggcggt ggggcaactg gtgggtacgg ggtggagggg ttttgattcg
     2701 gcgcatattg tgggagttat tgccgacttc cataacgaag ggttgcaaaa ggccattcag
     2761 ccgttggtgt tgcttccgca cagtgacggg cgcgcgaatt attccctcaa ggtacaggtg
     2821 gcggacgccg gggcaaccgt tgcggcgata aaggcgatct gggaccggca tttcccgggt
     2881 gacccctaca attatttttt cctcgacgaa ttctttagcc ggcagtatgc cgagaaccag
     2941 cgttttggag aagtgttcgc gctgtttgcc gtgtttgcgg tggttatcgc ctgttttgga
     3001 ctgctggggc tttcggcgaa taatgtgtta cagcggacga aggagatcgg tatacgcaag
     3061 gtgcttggcg cctcggtcaa cagtttgctt tatttgttgt cccgggattt tcttgtgctg
     3121 gtggtcgtgg cgcttgtcat tgccgttccg gttacctgg
//
LOCUS       DATGFG010000889         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_960824, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000889 DATGFG010000000
VERSION     DATGFG010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960824"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VKQ52_16775"
     CDS             <1..477
                     /locus_tag="VKQ52_16775"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HLZ88913.1"
                     /translation="KPVIDIDIIVEKPERLRDVILQLEGLGYIHAGDLGIEGREAFSR
                     TSSEVPVSHPRREWPPHHLYVCRQGIESLQNHLLLRDYLRNHPEKAREYSALKEELLK
                     RVNDDIDLYVEGKSEFIATVLEKAGLPAASITRMRGQNKAPVPDGERIQIVRNQSA"
     gene            655..1539
                     /locus_tag="VKQ52_16780"
     CDS             655..1539
                     /locus_tag="VKQ52_16780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLZ88914.1"
                     /translation="MEIKKENIKQEVFDLYDDYAHNRLSRRGFMEKLSLYAVGGITVS
                     ALMSFLMPDYKNSVQVKADDPRLKSEYITYSSPKGGGTIKALLSQPADMTKKLGGIVV
                     VHENRGLNPYIEDVGRRAALAGFVSIAPDALTPLGGYPGDDDKGREMQSKRDKNEMLE
                     NFIDAFGYLKKHPDCNGKVGVVGFCFGGGIANMMAVRLPDLAAAVPFYGAQPPLELVP
                     QIKAPLLLHYAGLDTRVDEGWPAYENALKENKKEYTAYIYPGVNHGFHNNTTPRYDKD
                     AAELAWKRTIDFFTEKLS"
     gene            complement(1590..2483)
                     /locus_tag="VKQ52_16785"
     CDS             complement(1590..2483)
                     /locus_tag="VKQ52_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88915.1"
                     /translation="MRIFVYPLLILSCLYQPVLGQEYRIPFQLTEYNNLSVQAILNRQ
                     DTLHLMFHTAENSVTLTEEAAKKSKSLRFDGLVDSVKSWGGQGNSSRFSKNNALQIGG
                     LSWEGLTVWEDQHSGRNTDGKFGPNLFVDRVIEIDFDTKMITLRPDLPDKIGEYEKLK
                     LQVEGHSLYIQATCQIGNDSFTNRFLVHSGYSGAVLLDDQFVSDHHLEERLTVVDGKE
                     LKDAYGNVLKTRKAIMPGFRIGNGRLADVPVGFFQGAIGRQKISVIGGDVLRRFNIII
                     DARREYIYLKPNSLQKVAYWS"
BASE COUNT          667 a          667 c          643 g          620 t
ORIGIN      
        1 aagccggtaa ttgatattga tattattgtt gaaaaaccgg aacgcctgcg cgacgttatc
       61 cttcaattgg aaggtctggg gtatattcat gcgggagacc tgggtatcga gggcagggag
      121 gccttcagcc ggacatcctc cgaagtgccg gtcagtcacc cgcggcgaga atggccgcca
      181 caccacctgt atgtctgccg gcaggggatc gaaagcctgc aaaatcacct tttactccgg
      241 gattatcttc gaaaccatcc tgaaaaagca cgcgaatatt cagccctgaa ggaagagttg
      301 ctaaaaagag tcaacgatga tatcgacctc tatgtggaag gcaaatctga gtttatcgcc
      361 actgtactgg aaaaagccgg attaccggcg gcttcgataa cccgtatgag ggggcaaaat
      421 aaggcaccgg ttcctgacgg agaaaggata cagatagtgc ggaatcaatc agcctgaagc
      481 cattgcgctt tatcaaaaga acaactatca cccgattccc aggttcggca ggtataggga
      541 cagcgccaac agcgtatgct tccggaaaga cctgtagttc accgattaga tgcccctgac
      601 tgatccgaat ctcttttatt tggcataaat tggcataaac aaatttgcct ctatatggaa
      661 ataaaaaagg agaacatcaa acaggaagta ttcgacctgt atgatgatta tgcgcataac
      721 cggctgagca gacggggttt tatggaaaaa ctctctttat atgccgttgg gggaattacg
      781 gtttcggcat tgatgagctt tctcatgccc gattacaaaa attcggtaca ggtaaaggcg
      841 gatgacccaa ggctgaagtc ggaatatatc acgtattcct ctccaaaggg cggcggtacc
      901 atcaaggcat tgctttccca acccgccgat atgactaaaa agctgggagg tatcgtcgtt
      961 gtccatgaaa accggggatt gaatccctac atcgaagatg tcggcagaag ggccgcattg
     1021 gcggggttcg tctcgatagc cccggatgca ttgacgcccc tgggtggtta tccgggcgat
     1081 gatgacaagg gccgcgaaat gcagagcaaa agggataaga acgaaatgct ggagaatttt
     1141 atcgacgctt tcggctatct caaaaaacat cccgattgca acggcaaagt aggcgttgtt
     1201 ggtttttgtt ttggcggcgg gattgccaat atgatggcgg tacgcctccc cgatctggcg
     1261 gcagccgttc cgttttatgg agcgcagccg ccattggagc tggttccaca gataaaggcg
     1321 cccctccttt tgcattatgc cggattggat acgcgtgtcg atgaaggatg gcctgcatac
     1381 gaaaatgccc tgaaagaaaa caagaaggag tacacggcct atatctaccc gggagtgaac
     1441 cacgggtttc ataacaatac gactccccgc tatgataagg atgccgcgga gctcgcctgg
     1501 aaacggacca tcgatttctt tacggaaaaa ctaagttgaa acctaaattc agcctggtcg
     1561 aaatccgcgc ttcacccggg caccgatccc tagctccaat acgccacctt ttgcaaacta
     1621 tttggtttta gatagatgta ctccctccgg gcgtcgataa tgatattgaa ccttctcagc
     1681 acatcacccc cgattacgct gatcttctgc ctgccgatcg cgccctgaaa aaaccccacg
     1741 ggcacatcgg ccaaacgccc attcccgatc ctgaacccgg gcatgatcgc cttccgggtc
     1801 ttcaatacat tgccataggc atcctttaat tcctttccat ccactaccgt tagccgttcc
     1861 tcgagatgat ggtcgctcac gaactgatcg tcgagcagaa cagcccccga atatcccgaa
     1921 tggaccagga accggttcgt aaaactatcg ttcccaatct gacaggtagc ctggatatac
     1981 agactatgac cttccacctg taatttgagc ttctcatatt ctccgatctt atccggcaga
     2041 tcggggcgaa gggtgatcat ctttgtgtcg aaatcgatct cgatcaccct gtcgacaaac
     2101 aggttcggac cgaatttccc gtcggtattc cggccggagt gctgatcttc ccataccgtc
     2161 agcccctccc agctcagccc gcctatctgc agcgcgttgt tcttgctgaa cctcgaagaa
     2221 ttcccttgcc cgccccagct ttttacacta tcgaccaagc cgtcaaatcg caggctttta
     2281 ctttttttcg ccgcctcttc cgtcagggtg accgaattct ccgcggtatg aaacatcagg
     2341 tggagggtat cctgtctgtt caggatcgcc tgtacggata gattgttgta ctcggtcaat
     2401 tgaaacggga tgcggtattc ctggccgagc accggctggt acaaacagga aaggataaga
     2461 agggggtaaa caaaaatgcg catgtcgaca gatttatatg tttattcaat aatactatcc
     2521 agattcaggt caagcaattg cccattgatc ggatagtcgc cgctttccca tttttccctc
     2581 ggaaatgggg gcagggt
//
LOCUS       DATGFG010000890         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_221007, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000890 DATGFG010000000
VERSION     DATGFG010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221007"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="VKQ52_16790"
     CDS             <1..1776
                     /locus_tag="VKQ52_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ88916.1"
                     /translation="PWAHLRREGDDDQNWGNNSTTTYVLLKPNTSLASLAPKMKVLKT
                     RYDSTEKRWEMFLYPMSRWRLYSNFKGAEEDGGRIEFVRLFAIIACFIVLIACINFMN
                     LSTARSERRAKEVGIRKVVGAPRSRLIGQFIGESVLLSFLAALLAIGIVLVSLPGFNA
                     LTDKQLYVPFGNVWFWLSAVAFILFTGLLAGSYPAFFLSSFQPVKVLKGTFRAANALV
                     TPRKVLVVLQFTFAIVLIICTIIVTQQINHARDREVGYDRANMMYHFMTGDIGKNYPL
                     IRQELLSSGVALSVCRTNSPWTQRYSDTWGFQWKGKDPEDKTDFIRENADEGIVATTG
                     IKLVEGRDLDLVHYPTDSNGILLNESAAKAMHFKDAIGQEITDDDIHFHVVGVIRDFI
                     IESPYTPIRPLVILGAKEPWYNVLNIRFNNKSLTETNLKKAEAIFRRYNPQYPFEYKF
                     VDEEYSRKFDDEKRIGTLAGLFAVLTIFISCLGLFGLATYMAENRVKEIGVRKVLGAS
                     VAGITALLSKDFLQLVLVSFLVAAPVAWFAMYKWLQDYPYRVRIEWWVFALAAVLSML
                     IALLTVSFQAIRAGMANPARSLRSE"
     gene            1844..>2695
                     /locus_tag="VKQ52_16795"
     CDS             1844..>2695
                     /locus_tag="VKQ52_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88917.1"
                     /translation="MKKTALSFVLAIAAMGSLFAQKDSSYYDLGYTTLDKRFTQSITV
                     KGSDLEKMPFVNLGDAIGAWFYGSYTEPGMVIYIVDGNPVSDVNTYSIFDIEEVTLVQ
                     NAAALVGTAGSQQEVVLVKTKRGNGGRGLLAAGQLGLVDGNGGGVKTNTSLYHHYYLG
                     VYANKEKMSYGISADWVRDIEPVTKDSRTEVRTPFNLQRWRLNGYFNWRPDSRNSIDV
                     VMGYAPQRIAEDVTGKTSPTMQELSNNAHAHVLSPQVRWHSELPAGFTNDLQGTYLAT
                     TGNGDYYE"
BASE COUNT          565 a          602 c          887 g          641 t
ORIGIN      
        1 ccctgggctc atcttcgccg ggagggggat gatgaccaga actgggggaa taattccact
       61 acaacgtatg tgttgctgaa acccaatacg agtcttgcat cgctggcgcc gaagatgaag
      121 gtgttgaaga cgcgctatga cagtaccgag aagcggtggg agatgttcct ttacccgatg
      181 agccggtgga gactgtattc caacttcaag ggggcggaag aggatggcgg gaggatcgaa
      241 tttgtgcgcc tgtttgccat cattgcgtgt tttatcgttt tgatcgcttg tatcaatttt
      301 atgaatcttt cgactgcgcg gagtgagcgg cgggcgaagg aggtggggat ccgcaaggta
      361 gtgggggcgc cgaggagccg gctgatcggt caatttatcg gggagtcggt gttattgtct
      421 ttcctggcgg cgttgttggc gatcgggatc gtgctggtga gtctgccagg tttcaatgcg
      481 cttacggaca aacaactgta tgttcctttt ggcaatgtgt ggttctggct gtcggcggtg
      541 gctttcatcc tgttcacggg tctgctggcg gggagttatc cggccttttt cctttcctcc
      601 ttccagccgg tgaaggtgct gaagggaacc ttcagggcgg cgaatgcgct cgtgacccca
      661 cgcaaggtac tggtggtatt gcaattcacc tttgccattg tcctgatcat ctgtacgatc
      721 atcgttacgc agcagatcaa ccatgcccgg gaccgggagg tgggatatga tcgcgccaat
      781 atgatgtatc attttatgac cggggatatc gggaagaact acccgttgat caggcaggag
      841 ctgttgtctt cgggggtggc tttatcggtg tgccggacga acagcccgtg gacgcagcgg
      901 tacagcgaca cctggggctt tcaatggaag gggaaggacc ccgaggacaa gaccgatttt
      961 atccgggaga atgccgatga ggggattgtt gcgacgacgg gcatcaagct ggtggagggg
     1021 cgtgacctgg atcttgtgca ctatccgacg gacagtaacg gcatactgct gaacgagtcc
     1081 gcggcgaagg ccatgcattt caaggacgcg atcggccagg agatcacgga tgacgatatc
     1141 catttccatg tggtgggagt gatccgggat tttatcatcg agtcgcctta taccccgatc
     1201 cggccgctgg tgatcctcgg ggcgaaggag ccatggtaca atgtcctgaa tatccggttc
     1261 aacaataaga gcctgacgga gaccaacctg aagaaggcgg aggctatttt ccggaggtat
     1321 aatccccagt atccattcga gtataagttt gtcgatgagg aatacagccg gaagttcgat
     1381 gatgagaagc ggatcggtac gcttgcgggg ctttttgccg tattgacgat ctttatctct
     1441 tgtcttgggt tgtttgggct ggcgacctat atggcggaga accgggtgaa ggagatcggg
     1501 gtgcggaagg tgttgggggc ttcggtggcg gggatcacgg ccctgttgtc gaaggatttt
     1561 ctgcagctgg tgctggtgtc cttcctggtg gcggcgcctg tcgcctggtt tgcgatgtat
     1621 aaatggctgc aggattatcc ctaccgggtg aggatcgagt ggtgggtatt tgcgctcgcg
     1681 gcggtgttgt cgatgttgat cgcgttgctg acggtgagtt ttcaggcgat cagggcgggt
     1741 atggcgaatc ccgcgaggtc tttgcgaagc gaatgagctg taccgggggg ttttgtttgc
     1801 ggtcggatca gtatatttat ggcaaataag cctaaaccgt tttatgaaaa agaccgcctt
     1861 atcattcgta ctggcgattg ccgccatggg aagcctgttc gctcaaaagg atagttccta
     1921 ttatgatctc gggtatacta cgctggacaa aagattcact caaagtatca ctgtaaaggg
     1981 cagcgacctg gagaagatgc cctttgtcaa cctgggcgat gccatcggcg cctggtttta
     2041 cggcagctat accgagccgg ggatggtgat ctatatcgtc gatggcaacc cggtgagcga
     2101 tgtgaatacc tatagtattt tcgatatcga ggaggtgacg ctggtgcaaa acgcggcggc
     2161 gttagtgggt acggccggtt cgcagcagga ggtggtgctg gtgaagacga agcgggggaa
     2221 cggcgggagg ggattgctgg cagcgggcca actggggctt gtcgatggca atggaggcgg
     2281 ggtaaaaacg aatacgagcc tgtatcatca ctattatctt ggtgtgtatg ccaacaagga
     2341 gaagatgagc tatggcattt cggccgactg ggtacgcgac atcgaaccgg tgacgaagga
     2401 ttcgcgcacg gaggtccgga cgcctttcaa cctgcagcgc tggagactga atggctactt
     2461 caactggcgg cccgattccc ggaacagtat cgatgttgta atggggtacg ccccgcagcg
     2521 gatagccgag gatgtgaccg ggaagaccag tccgacgatg caagagctat ccaacaacgc
     2581 gcatgcgcat gtcctgagtc cgcaggttcg ctggcatagc gagctgccgg ccgggtttac
     2641 caatgacctg cagggaacct acctggcaac caccggtaac ggtgattatt atgag
//
LOCUS       DATGFG010000891         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_994945, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000891 DATGFG010000000
VERSION     DATGFG010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994945"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..606
                     /locus_tag="VKQ52_16800"
     CDS             265..606
                     /locus_tag="VKQ52_16800"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLZ88918.1"
                     /translation="MIYSPELVKGTLKTIVLKLLSDNKKMYGYEITQKVKELTGDRIQ
                     ITEGALYPTLHALENDGMVTTQVEYIGKRVRKYYSLSPAGKTMSKERIKELAAFMSTM
                     SFLLDIKPKTA"
     gene            640..1176
                     /locus_tag="VKQ52_16805"
     CDS             640..1176
                     /locus_tag="VKQ52_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88919.1"
                     /translation="MYHLTDQQIDFILDDIRAEGITMEDLQQNLLDHACILIEENLEE
                     GGDFMAYYTSTIRSFYRQELRELEEEAIFLDRHPLHILLSRGQFFVLLFALFIGPYIA
                     YDINWAFTTGRVNGFCLPMEVLAGTFVFALFPLLTLLVLLLTPDRLDPVIPRRAKILL
                     GFRPFIRIIPEHPPFHTR"
     gene            complement(1142..1495)
                     /locus_tag="VKQ52_16810"
     CDS             complement(1142..1495)
                     /locus_tag="VKQ52_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88920.1"
                     /translation="MRYRIGYSIVPGQHIPDKCFTFSEIPLLSSPNSFTCALYDADRV
                     ALQTLQATTGTVVSTPVQIAGLPGTILYVHLNHSGSNEPDIIVPLELYGKGGLAALNR
                     KNIAFTWYEKEDVPE"
     gene            1751..>2103
                     /locus_tag="VKQ52_16815"
     CDS             1751..>2103
                     /locus_tag="VKQ52_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88921.1"
                     /translation="MRQDDALWKSILESTFEDFLRFFFRDADEVIDFHRGFEFLDKEL
                     AQLCISGRRRAPKVIDKLAKVYMRGGKEEWLLLHIEVQGYREPGFEKRMFRYCYRLMD
                     KYEKPITAIVILTDP"
BASE COUNT          547 a          514 c          519 g          523 t
ORIGIN      
        1 ccgccgccct ccggtaccgg atatggtatg caatgcaata tgcttcccgc gccatccgac
       61 cgggatttcg aagagcctgt cctgagccgc caatctattc aataggaaaa aaacgaaaaa
      121 gaaaagggcg atctgcttca tacggttggt aaaataaatc atgattgtaa tcaaaggtca
      181 tgtcaggctt gcaaaatagg attttctacc gcaaaataca tctaattctt atgtatattt
      241 gtttaaggaa aaaatcaccc atccatgatc tactctcctg aattggtcaa agggaccctg
      301 aaaaccatcg tcctgaaatt attatcggac aataaaaaga tgtatggcta tgagatcacg
      361 cagaaggtca aggaactgac cggcgatcgt atccagatca ccgaaggggc gctctatcct
      421 accctgcacg cattggagaa cgacgggatg gtgaccaccc aggtggaata tatcggcaag
      481 cgcgtccgga aatattacag cctgagccct gccggcaaga cgatgagcaa ggagcggatc
      541 aaggagctgg ccgcattcat gagcaccatg tcgttcctgc tggatataaa gcccaagact
      601 gcttagcgca atacatcaac tcatcctaat accgatgcca tgtaccatct caccgaccaa
      661 cagatagatt tcatcctcga tgatatccgc gcggaaggta tcaccatgga ggatctgcag
      721 caaaacctgc tcgatcatgc ctgcatcctt atcgaagaga atcttgaaga gggaggtgat
      781 ttcatggctt attacacatc gacgatccgc tcattctacc ggcaggagct ccgcgagctg
      841 gaggaagagg ctatcttcct cgaccggcat ccccttcata tcctgctcag caggggccag
      901 ttcttcgtgc tgttattcgc gctcttcatc gggccgtata ttgcttacga tatcaactgg
      961 gcctttacta ccggccgcgt caacggcttt tgcctgccca tggaagtgct ggccggcacc
     1021 tttgtatttg cgcttttccc cttgctgacc ctgctggtgc tgctgctgac acctgaccgc
     1081 ctggatcccg tcattcccag gagagcgaag atactgctgg gcttcaggcc gttcatccgg
     1141 atcattccgg aacatcctcc ttttcatacc aggtaaaggc gatatttttc cggttcagcg
     1201 cggcgagacc gcctttgccg tacagctcca gcgggacgat tatatccggc tcattgctgc
     1261 ccgaatggtt caggtgcaca tagaggatgg tgcccggcag accggcaatc tgaacaggcg
     1321 tggagacgac cgtgccggtc gttgcctgca aggtttgcag cgcaaccctg tcggcatcat
     1381 acagtgcaca ggtgaatgag ttgggcgacg aaagcagcgg gatctctgag aaggtgaagc
     1441 atttgtcggg aatatgctga ccaggcacaa tgctatatcc gattcggtat cgcataagca
     1501 gtaatttgtt cccgtagatt aaagcttata aaaaaatcct ttttaagtat aaatacgcca
     1561 attcgcccag gtataagtac gcaattttgc ctggcggtct gcccgactgg aaaccctcgc
     1621 ctgggttccc tgcactcttc catacggagg gaaaggaaaa aatttttttt tctcattcct
     1681 tgatcttcgt caaacaggtc aatctccttt tcttcgaact ttgcgtcgac ccaaaagaga
     1741 ggagttgttt atgaggcagg atgatgcgtt atggaaaagc attttggaaa gtacttttga
     1801 agacttttta aggttcttct ttcgggacgc agacgaagta atcgatttcc atagaggttt
     1861 cgaatttctt gacaaggagc ttgctcaact atgcatttca ggcaggagga gggcgccaaa
     1921 agtcatagac aagctggcga aggtttatat gcggggtgga aaagaagaat ggcttctgtt
     1981 acatatcgaa gttcagggct accgggaacc tggttttgag aagagaatgt tcaggtattg
     2041 ttatcgcctg atggataaat acgaaaaacc gatcacggca attgttatcc tgacagatcc
     2101 gca
//
LOCUS       DATGFG010000892         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_277292, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000892 DATGFG010000000
VERSION     DATGFG010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277292"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VKQ52_16820"
     CDS             complement(<1..409)
                     /locus_tag="VKQ52_16820"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HLZ88922.1"
                     /translation="MQIKPFQIEITDSQLYDLQHRLDNTRWPEELPGTGWERGIPVAW
                     LRRMAGYWRHQYDWRKQEALLNRFPQFTATIDGQTIHFLHIRSREAGAKPLIITHGYP
                     SSIVEFIDLIGPLTDPRAHGGRSSDAFHLVIPSL"
     gene            complement(501..1418)
                     /locus_tag="VKQ52_16825"
     CDS             complement(501..1418)
                     /locus_tag="VKQ52_16825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLZ88923.1"
                     /translation="MRKDIWPLVAGLLFSLLWSSAATATKVGLQFVQPFTICVVRFFI
                     AGIVMIVISHAVLRQRLPRGREWGHIAVYGLLSNSVYLGLYVFAMRQVSAGLGSLAVA
                     TNPLFINLLTAIFLRQRLSATTIFCLVLCMAGVTLAAWPLFRNSSATPVGLLILLSGM
                     IAYSVSVYYFSRIRWNGLPLLAINGWQVLFGGVFLLPLAAAVYQPVLNGWNMKALGAI
                     VWLAIPVSIGAVQLWLYLLKQDAARSSFWLFLCPVFGFAISNIFTGEPITAYTIVGMG
                     LVIVGIYWQNARKEGRPERRSKEQTERLE"
     gene            complement(1444..2670)
                     /locus_tag="VKQ52_16830"
     CDS             complement(1444..2670)
                     /locus_tag="VKQ52_16830"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HLZ88924.1"
                     /translation="MKTICLLMCLPAFAGFLHAQPGAYSFRVRTITAGVTPAGPRDTG
                     ALLRAIHFLTEAKKEFTNKGYEVQTLRVATSNLYTWLAGHSLEEVMDNLGQWDQIARR
                     NGVILSIGQVLAPDQYQAGISDWAKRLVRSTQTISFSLAISSHSLGIHGQSIRAAAEI
                     ILALSTVDNGEANFRFTASANCPAGIPFFPAAFHEGGNSFAIGLESPNLLTEAFAQSD
                     RSNARQVLKALLEKNFRPVQSLGEQIAAGSGWVYDGIDVSAAPGLDASIGKAVETYTK
                     QPFGSPSTLGACALITDVLKGLDIKKCGYSGLMLPVIEDPVLAQRAEEKRYTVGELLL
                     FSSVSGTGLDVVPIAGDTPPGVIEGILTDVAALSLKYTAKALSARLFPLPGKKAGDRV
                     EFKNPRLTATTVMSIN"
     gene            2778..>3683
                     /locus_tag="VKQ52_16835"
     CDS             2778..>3683
                     /locus_tag="VKQ52_16835"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HLZ88925.1"
                     /translation="MIPVKGYAAQTPTTDLAPWDFQRREVGPHDVQFDIQFCGVCHSD
                     LHQIKNDWFPGIFPMVPGHEIVGRVIKVGSHVKKFKVGDLAGTGCLVDSCRECENCKQ
                     DLEQYCLNGNSQTYNGLEQDKKTPTYGGYSKTIVVHEDFVLHLSAKLDLAATAPLLCA
                     GITTYSPLRHWKIGKGHKVAIVGLGGLGHMGVKFAVSFGADVTVMSTSPNKEKDAKKL
                     GAHHFLVTTDPNQIKAAANSFDFILDTVSAEHDLNMYMSMLKTDGTHCLVGVPSKPLP
                     VHPFTVIGGRKSFAGSGIGGLKETQE"
BASE COUNT          884 a         1167 c          928 g          704 t
ORIGIN      
        1 gcaaagaagg gatcacgagg tgaaaggcat cggaagaccg gccgccatgt gcccgcggat
       61 cggtcaacgg accgatgaga tcgataaact cgacaatgct gctgggatac ccatgcgtga
      121 tgatcaaggg ctttgcccca gcctcccggc tccggatatg cagaaaatga atggtctgcc
      181 catcgatagt tgctgtgaac tggggaaacc ggttcagcaa cgcttcctgt ttgcgccagt
      241 catattgatg ccgccaatag ccggccattc gccgcaacca ggccaccggg atacctcgct
      301 cccagccggt gcccggcaat tcctccggcc atcgggtgtt atccagccga tgctggaggt
      361 catataattg ggaatcagtg atctcgatct ggaaaggctt gatctgcatg gcattgattt
      421 tttggtgtta aaaaggatta ccccaccaaa ttaccatggc cggctgtcag ccttatgtca
      481 gtaggatgcc cggttctttt ttattccagc cgctccgtct gctcctttga tcgccgctcc
      541 ggccgccctt ccttcctcgc attctgccaa tagataccca cgatcactaa ccccatccct
      601 acgatcgtat aggctgtgat cggctcaccc gtgaaaatat tggagatcgc aaaaccaaac
      661 accgggcaaa ggaacagcca gaaggacgaa cgggctgcat cctgcttcaa tagataaagc
      721 cacagctgca ccgcaccgat cgatacgggg atcgcaagcc acacgatcgc ccccagcgcc
      781 ttcatattcc acccattcaa caccggctgg taaaccgccg ccgccagcgg aagcaggaag
      841 accccgccaa aaagcacctg ccacccattg atcgccagca ggggtaaccc attccatcgg
      901 atccgcgaaa aatagtacac actcaccgaa tacgcgatca tccccgacaa caggataagc
      961 aaacccaccg gcgtcgcact gctgttccgg aacagcggcc aggccgcgag cgtaaccccc
     1021 gccatacaaa gcaccaggca gaagatcgta gtggcggaaa gccgctgccg cagaaagata
     1081 gccgtaagca ggttgataaa aagcggattg gtcgccacgg ccagtgaacc cagccccgcc
     1141 gatacctggc gcatcgcaaa aacatacaat ccaagataga cggagttcga caggagtccg
     1201 taaaccgcga tatgccccca ttccctgccg cggggcagcc gctgacgaag tacggcgtga
     1261 ctgataacga tcataacgat cccggcaata aagaaacgga cgacacagat ggtaaacggc
     1321 tggacgaact gcaatccgac cttcgttgct gtcgcggccg aggaccaaag aagagagaac
     1381 aacaaacccg caacgagcgg ccagatatct tttctcaacc cgattttttg gtgaatatat
     1441 aacttaatta atgctcatca ctgtcgtcgc cgtcagccgc ggattcttaa actccacccg
     1501 atctcccgcc ttcttacccg gcagcgggaa caatctcgcc gaaagcgcct tcgccgtata
     1561 cttcagggac aacgccgcga catccgtcag aatgccttcg atcaccccgg gaggcgtatc
     1621 tcccgcgatc ggcacgacat ccagcccggt gccggagact gaagagaaca acagcaattc
     1681 ccccaccgta taccgctttt cttccgcccg ctgcgccagc accgggtctt cgatcaccgg
     1741 cagcatcaaa cccgaatagc cgcatttctt tatatcgagg cccttcagca catccgtgat
     1801 caaggcgcag gcgccaagcg tagaggggct gccgaacggt tgctttgtat aggtctccac
     1861 ggccttaccg atactcgcat ccagccccgg tgcagccgac acatcgatcc cgtcatacac
     1921 ccacccactc ccggccgcga tctgctcacc cagggactgc accggcctga agtttttttc
     1981 gagcagcgcc ttcagcacct gccgggcatt gctccggtcg ctttgtgcga acgcttcggt
     2041 cagcaggttg ggcgactcca gcccgatggc aaagctattc ccgccttcat gaaaggccgc
     2101 gggaaagaac gggatgccgg ccggacaatt ggccgaagcc gtaaatcgaa aattggcctc
     2161 gccgttatcc acggttgaca gcgccaggat gatctccgcc gcagcccgga tcgactgccc
     2221 atggataccc agggaatgcg aagagatcgc caggctgaag ctgatcgtct gggtgctgcg
     2281 gacaagccgc ttcgcccaat ccgagatccc cgcctggtat tggtccggcg ccagcacctg
     2341 cccgatggag aggataactc cgttcctcct ggcgatctgg tcccattgcc cgagattgtc
     2401 catcacttcc tcaagcgaat gccccgccag ccaggtatat agattcgaag tggccacccg
     2461 caaggtctgc acctcatacc ccttgttcgt aaattccttt ttcgcctccg ttaaaaaatg
     2521 aatggcccgc aacaacgcac cggtatcccg gggtccggcc ggcgtcactc ccgccgtgat
     2581 ggttcgcacc cggaaggaat aggcccccgg ctgggcgtgt aaaaacccgg caaaggccgg
     2641 caaacacatc aggaggcaaa ttgttttcat acacctaaat atactcccat ttaccaccaa
     2701 aaaaaatatg agttaccccg ggttgtctat tttatcggca gatttgtcta acttttcccc
     2761 cactaaaaaa aggaactatg ataccagtaa aaggctatgc tgcacagacc ccgacaaccg
     2821 acctggctcc atgggacttc caacgccgcg aggttggccc gcatgacgta caatttgaca
     2881 ttcaattctg cggagtatgc cactccgatc tccaccagat caaaaatgac tggttccccg
     2941 gcatcttccc gatggtcccc ggtcacgaga tcgtcggcag ggtgataaag gtcggcagcc
     3001 acgtgaaaaa attcaaggtc ggcgacctgg cgggcaccgg ctgcctcgtc gactcctgcc
     3061 gcgaatgcga gaactgtaag caggatctcg aacagtattg tctcaacggc aattcccaaa
     3121 cctacaacgg actggaacag gataagaaga cgcccaccta cggcggctat tcgaagacca
     3181 tcgtggtgca cgaggacttt gtcctccatc tctccgcaaa gctggacctc gccgcgacgg
     3241 cgcccctgct ctgcgccggc atcactactt attctcccct ccgccactgg aagatcggca
     3301 aggggcataa ggtggccatc gtcggcctgg gcggtctggg ccatatgggg gtgaaattcg
     3361 ccgtttcctt cggcgcggat gtcaccgtca tgagcacctc gcccaataag gaaaaggatg
     3421 caaagaaact gggcgcccac cacttcctgg tcaccacgga ccccaatcag ataaaggctg
     3481 ccgccaatag cttcgacttc atcctcgata ctgtctccgc cgaacacgat ctcaacatgt
     3541 atatgtccat gctgaagacg gatggcacac attgcctcgt gggcgtgccc tcaaaacccc
     3601 tgcccgtgca tcccttcacc gtcatcggcg gccgtaagag cttcgccggc tccggcatcg
     3661 gtggtttgaa ggaaacgcag gaa
//
LOCUS       DATGFG010000893         9327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1093759, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000893 DATGFG010000000
VERSION     DATGFG010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9327
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093759"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VKQ52_16840"
     CDS             <1..528
                     /locus_tag="VKQ52_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLZ88926.1"
                     /translation="AGKDPRDIRRIIITHAHTDHTGSAAAISKELNIPVWSHADTARL
                     VEQGRPGEAPLVVSPGLMNQLIYQLFIRRGGTIDPIAITRRLIDGETLPIGGPGGVKV
                     IYTPGHGAGHLALFLQADRVLIAADICANAGGLGWSTVYEDRELGRKSILKAAAFDFD
                     IAVFGHGRPLTPTCH"
     gene            717..1163
                     /locus_tag="VKQ52_16845"
     CDS             717..1163
                     /locus_tag="VKQ52_16845"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLZ88927.1"
                     /translation="MNYEQKIREAEDRLSIRELVDNYAYCADTRDAQGQMALFTEDTN
                     FEVYYDPKSGTPSQVITKKEDLFPVFDNLNTYIATMHFNGQSTVRLNGDKAVGTTYCM
                     AHHVTIEEGKQKLMVAAIRYHDAFVKQNDKWLFAERKLLVDWIENR"
     gene            1328..3958
                     /locus_tag="VKQ52_16850"
     CDS             1328..3958
                     /locus_tag="VKQ52_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88928.1"
                     /translation="MQFYRTDMIDKALEIMDRTRDLPKEQQFSWTAPGWVMAKVLEDW
                     PGQTQQRHQRLEEAFQSGRFVCHAMPFSLQSQLLFPEDVARSYESSSFVSRKYGLPLP
                     RGAKMTDVPSQASVLATGLAQGGVKFMHIGCNWPSGDVKYPPLFWWQGPDGSRVLTLY
                     SPIYGTTVGLTYPREWSDANDPRVGSNLLPPANWPYRVWPAIFVTPDNSGPPGPNDVQ
                     ALFDEAAKKMPGVKIRVGRLEDFADAILPTHPDLPVIKAEAPDTWIHGCMSDPGGMKI
                     SRTINPFIPALEELNTQLSNWNVNVSDITKDISKAYENVLLYSEHTWGGAASVNKYGE
                     AFKKLPPDSYKDLEASWEDKTDYIRTTENIVRPLLATNLGLLAKNLNSKGIRYVVYNP
                     LPWPRSEWVEAGSPGSQHKFIYAENVPPSGYKTFTEKDLVVSNPVSVSANTIENKYFM
                     IELDPVKGVISSLIDKRTGRDWVDRKAECGLGQYMNERFTYEQTLKYVIDYQQGRAMN
                     IYGAKGVWPHPGMHKPGMISEKEVPYRAALSKNGSLKITKAPNEETALIDFPADTANH
                     FPASSLRITLQKDKPFVDMELTIKDKARDNWPEADWFCFPFKIEAPRFTVGRSLGMMD
                     PSTDIMEGADKDLYAVGTGVTLTDKDGSGIAFCPFDHCLISLDRPGIWKFTKDFIPHK
                     PVVYLNLYNNQWNTNYRYWYPGTWSSKVRIWTFTKNSTVESRLVTPSLEARTPLMVAE
                     ADGPAGSLPTEQAGISVTRKGIQITAFRHNSPENPVFATAAATALTATAPTTTATTPT
                     TPTAPTAPQSTLLRLWEQGGISGNVTVTLPNGSTHGKATPVNLRGEITGPPIRIEKGK
                     FTFLLRAYAPASFVLETDPD"
     gene            complement(3973..4881)
                     /locus_tag="VKQ52_16855"
     CDS             complement(3973..4881)
                     /locus_tag="VKQ52_16855"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ88929.1"
                     /translation="MPFQKHTHRKRRGFEGQRLIVLPKKITTNFLTRDPVTRQVYITD
                     IGYYPKAKFHYVERSGGSSQHIIIYNVEGSGWVEIDGKRTTLSPSQFVVIPADAPHKY
                     AANEADPWTIYWVHFTGRMASYIVGLLRQNSKSYKPDLAYSENRIRLFEDIYSNLEKG
                     YGSDNLRYINMIFYHFLSSLLYEDKFTSSEKKKEGDVIAGTIELMQKKIHTVVSLQEL
                     ARFAHLSVSHFSAVFRERTGYSPIEYFNHLKIQKACQYLSFTGMTVKETAASVGIEDQ
                     YYFSRMFSKLMGHSPMEYRKRGKTGK"
     gene            4995..7025
                     /locus_tag="VKQ52_16860"
     CDS             4995..7025
                     /locus_tag="VKQ52_16860"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HLZ88930.1"
                     /translation="MPKLIVSVFLCALLTLSSSAQPPAAPAETTAPAANAAPTLNADQ
                     VVTVLSQDGRTEVRITIGTTIRYSVAWQHRQVIDGSPISLIVDGVVLGQNAVVKAIYR
                     DSSRSVITPLYGKFARLTDEYRQLRIDFAQPFSLILRVYDEGIAWRWITSFPSDITVN
                     AEEVNVKLTSTDSGLLSVTPKLTTWELPYTPYATITEVPDSMNVIVPALFTDQQLRLV
                     VAESDLRDYPGMFLKHGPGGFHGYWAAYPKKETPYGGDWGMSTKVDEREDYIARTAGN
                     RSFPWRVIILADDDKRLLTSQLIYKLAAPSVIPDVSWIKPGKADWEWWHDAIVNDAGF
                     PTGMENRSTKLYEHYIDFAATHHLEYTLIDAGWSNFYHLDSPQRVDINQLVTYANQRN
                     VGLLVWCNATSLLGNLDHYMETFGKCGLKGIKVDFFDRDDQEIIRQQEQIAAAAAKWR
                     LLVDFHGSPKPTGLQRTYPNVINYEAVRGEECSKWDTTSNPQYHLQIPFIRMLGGDLD
                     YTPGSMRNRSKAAFVPIPNGLPTTMGTRCHELALFILFDQYLAVLSDSPEAYRKYADI
                     MAFLSAVPVSFDDTRILAAKLGQYVVLAKRKGTDWYVGALTNWTGRDVTLDLSFLDQG
                     NYKADILKDGADAGANAESYTNKKTTVDQHTQLHLHLASGGGAVIKISKASI"
     gene            7022..>9327
                     /locus_tag="VKQ52_16865"
     CDS             7022..>9327
                     /locus_tag="VKQ52_16865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ88931.1"
                     /translation="MTKNLLLLLLGVCTILVHAQGQTPGNGGSLTIRGTVRERNSGQA
                     LAGVQISQKTDPKNVVVAGAAGEFTITVPALHTILVFHAMGYTDLEMDVAGRTSLTVD
                     LESGGAGNLDEIIVVGAVVKRHDLTGSVATVDAKTIGITPTSDINTAIQGKVAGAYVV
                     NSAQPGTAASIRIRGNNSLSYGTSPIYVVDGVIVGDNLNSLNPDDIADMEVLKDASAT
                     AIYGSRGANGVVLVSTKKGRKNTGEVHYNSWVSWSEFARKMKFMNAQQIYNLRADAFA
                     NQYMDDNPGADRQAYLNRITSDTSNLVFADYEKQTIKSGRSYDWLHAVSQNGMAQNHT
                     LDFSGGSDKGTYFLSLNYTDQKGLIDGADYKRMGGRINIDENVKSWLKVGTYTTFTHS
                     ITDVADASVFPTAVAANPLLPVDTTGLYTLKWGDIFNQDQYNPLRSLYIQNNQYLNRL
                     LTSTYISVSPIKDLAIRTSFSADVTNEQQYTYIPTTTGQDKRNSNQGNAVHYKVENYN
                     LQWDNSATYAKTFGEHHLNFLVATSMQENRSSYNQITAYGFPNDDFGYMYLGGVTSRD
                     RTVLASDFVTTTLMSYMGRAEWNWKNTYYATATLRYDGSSKFGPSHQWGAFPSLAVAW
                     DMSREQFMSKFSVISHWKWRVGYGVAGNQNIPVYSTYSLYRPSYTNGTVTYVSDGRLG
                     NPDLQWERQKQVNVGTDIGLLNDRLNLTADYFNIKNDNLLIDESLAPTSGFTNKVANV
                     GAMTNKGIEFTLDYAIVKHKDLQWNFRF"
BASE COUNT         2368 a         2687 c         2318 g         1954 t
ORIGIN      
        1 gccggcaaag acccccgcga catccgccgt attatcatta cccacgccca taccgaccac
       61 accgggagcg ccgccgccat tagcaaagaa ctcaatatcc cggtatggtc ccatgccgac
      121 accgctcgcc tcgttgaaca ggggcgcccc ggcgaagcac ctctggtcgt atctcccggc
      181 ctcatgaacc aactcatcta tcaactgttt atccgtcgcg gcggcactat cgatcccatc
      241 gctatcactc gccggcttat cgacggagag acccttccca tcggcggccc cggtggagtc
      301 aaggtcatct atactcccgg tcatggcgcc ggtcaccttg cccttttcct gcaggccgac
      361 cgtgtcctca ttgccgcgga tatatgcgcc aacgccgggg gcctcggttg gagcaccgtc
      421 tacgaagacc gcgaacttgg ccgcaagagc atcctgaaag ctgccgcctt cgacttcgac
      481 atagccgttt tcggccatgg ccgcccgctg acacccacgt gccactaatt cgaatcgcta
      541 aacaactcac ccgaccgctg agccttttag aaactacttt ttgggctttt cagaaagtaa
      601 cagcggcttt aatctttgga tatttaccgt acagatccat attgtcgatc actgatccct
      661 tatccggagc ggccccgccg gtcaaccgaa aatttagcaa taaaaaacta ataaatatga
      721 actacgaaca aaagatccgg gaagcggaag accgcctttc catccgggaa ttagtggaca
      781 attacgctta ttgcgcggat acccgcgatg cacaaggaca aatggcgctg tttacagaag
      841 ataccaattt tgaagtatat tacgacccga agtcggggac gccgagccag gtaattacta
      901 aaaaggaaga tctgttcccg gtattcgata atctgaacac ctacatcgcc accatgcatt
      961 ttaacgggca gagtacggta agactgaatg gcgataaagc cgtcggcacc acctattgta
     1021 tggcacatca cgtaaccatt gaagagggta agcaaaaact aatggtggca gccatccgct
     1081 accatgacgc ttttgtaaaa caaaacgata aatggctttt tgccgaacgc aaactactgg
     1141 tcgattggat cgaaaacaga taaattcttt aatgatagca aaaaaatatt ttgtcgttgt
     1201 attgctttcc ctcttgggag gcattcaaca gacgcccgcc cagactgtaa atccaaatgt
     1261 taaagagatt attgtaatat tcaagaccca cttcgatctt gggtataccc atcgcgcaaa
     1321 agatattgtg cagttttatc ggacggatat gatcgataag gcgttggaga tcatggacag
     1381 aaccagggac ttgccaaaag agcagcaatt ttcatggact gcacccggct gggtaatggc
     1441 aaaggttctc gaagattggc ctggacagac ccaacaacgc caccagcgat tggaagaggc
     1501 cttccaatcg ggtcggtttg tatgccatgc catgccgttt tcccttcagt cgcaactatt
     1561 atttcccgaa gatgttgccc gatcctacga atcgtcctcc tttgtctccc gtaagtacgg
     1621 gctgccattg ccgcgcggcg caaaaatgac ggatgtcccc tcgcaggcga gtgtcctggc
     1681 gacaggattg gcccagggcg gtgtgaaatt tatgcatatc gggtgcaact ggccaagtgg
     1741 ggacgtaaaa tatccgccgc ttttttggtg gcagggcccc gacggaagcc gtgtcctgac
     1801 actctattca ccgatttacg gcactaccgt gggactgacg tatccaaggg agtggagcga
     1861 tgcgaatgat ccacgcgtag gaagtaattt gctgccgcct gccaattggc cttatcgcgt
     1921 ctggccggcg atctttgtta caccggacaa ttccggccct cccggcccaa acgatgtaca
     1981 ggcattattt gacgaagccg cgaaaaaaat gccgggtgtg aaaataaggg taggacgcct
     2041 tgaagatttt gccgatgcca ttttacccac ccatccggat ctgccggtca tcaaagcgga
     2101 agccccggat acgtggatac acggttgcat gagcgacccc ggagggatga aaatttcgcg
     2161 tactatcaat ccatttatac ctgctttgga agagctgaat acacaactca gcaactggaa
     2221 tgtcaatgtt tcggatataa cgaaggatat ttcgaaagcc tatgagaatg tcttgctcta
     2281 tagcgaacat acatggggcg gtgcagcctc tgtcaataaa tatggcgaag catttaaaaa
     2341 actccccccc gacagttata aagaccttga agcatcctgg gaggacaaga ctgactatat
     2401 ccgcacaaca gaaaatattg taagaccact gctggcaacc aatctcggcc tccttgccaa
     2461 aaacctgaac agtaagggca tccggtatgt agtgtataat cctctcccct ggcccaggtc
     2521 ggaatgggtc gaagcaggca gcccaggcag ccaacacaaa tttatctatg cggagaatgt
     2581 cccaccgtcc gggtataaaa cctttacgga gaaagatctt gtcgtaagca acccggttag
     2641 tgtaagcgca aatacgatag aaaataaata ttttatgatc gaactcgacc ctgtcaaagg
     2701 ggttatttcg tccctgatag acaagcgcac gggaagggat tgggtcgatc ggaaggctga
     2761 atgcggcctg gggcaatata tgaacgaacg gtttacctac gaacaaaccc tgaaatatgt
     2821 gatcgattat caacagggtc gtgccatgaa catctacggc gctaaagggg tatggcccca
     2881 tcccggtatg cacaaacccg gaatgatctc tgaaaaagag gttccttacc gcgccgcctt
     2941 atccaaaaat ggctctctga aaattacgaa agcccccaat gaggaaacag cacttatcga
     3001 tttccctgcc gacaccgcca atcatttccc tgcttcatcc ttgcggataa ccttgcaaaa
     3061 agataagccc tttgtggata tggaactcac tatcaaagac aaggcaaggg acaactggcc
     3121 ggaagctgac tggttctgct tcccgtttaa aatagaggca ccccgattca ccgtcgggcg
     3181 ttctttaggg atgatggatc cttccacgga tattatggaa ggtgccgaca aagatctata
     3241 cgccgttggc accggggtta ccctcaccga caaggatggg tccggtattg ctttctgtcc
     3301 gttcgatcat tgcctgatca gcctcgacag acccggcatc tggaaattca caaaagattt
     3361 cataccccat aaaccggtgg tctatctcaa tctgtataat aatcaatgga ataccaacta
     3421 ccggtattgg tatccgggaa catggagctc aaaagtaaga atatggactt tcacaaaaaa
     3481 tagtaccgtg gaatcgaggt tggttacacc gtcgctcgaa gcccggacac cgttgatggt
     3541 agccgaagcc gatggccctg cggggagtct tccaaccgaa caagccggaa tttccgttac
     3601 gagaaaaggc atccaaatta cggcctttcg ccataatagc cccgaaaacc cggtgtttgc
     3661 cacagccgcc gccaccgccc tcacagccac ggcccccaca accacagcaa ccacccccac
     3721 aactcccacc gcccccactg ccccccaatc gacgctgctc cggctctggg aacaaggtgg
     3781 catctccggc aacgttaccg tgactctgcc caacggctca acccatggta aagccactcc
     3841 ggtaaacctt cggggtgaaa taaccggccc gccgatcagg atcgagaaag ggaagtttac
     3901 gttcttgctc cgggcctatg ctcctgccag cttcgtcctg gagacggatc cggactagct
     3961 ccggcgccga tcctatttcc ccgtcttacc tctctttcgg tactccatcg gcgaatgccc
     4021 catcagcttc gaaaacatcc gggagaaata atattgatct tcgatcccca cactcgccgc
     4081 cgtctccttc accgtcatgc ccgtaaagga aaggtactgg caggccttct ggatcttcag
     4141 atgattaaaa tactcgatcg gcgaataacc cgttcgttcg cgaaagacgg cggagaaatg
     4201 ggaaacggac aaatgggcga agcgcgccaa ttcctgcagg ctgaccaccg tatggatctt
     4261 tttttgcatc agctcgatcg tccccgcgat gacgtcacct tccttcttct tttccgaaga
     4321 ggtgaactta tcctcataca gcagcgatga aaggaagtga tagaagatca tgttgatata
     4381 acgcaggtta tcactgccat atcctttttc gagattggaa taaatgtctt cgaacagcct
     4441 gatcctgttc tcgctgtaag cgaggtcggg tttataactt ttgctgttct gccgtagcag
     4501 cccgacgata taggaagcca tcctgcccgt gaaatggacc cagtagatag tccatggatc
     4561 ggcctcgtta gccgcgtact tgtgcggggc gtccgccgga atgacgacga actgagatgg
     4621 agaaagagtc gttcgcttcc catcgatctc gacccagccg ctgccttcca cattgtaaat
     4681 gatgatatgc tggctgcttc cgccggagcg ctcgacatag tggaatttcg ccttgggata
     4741 gtagccgatg tcggtgatat agacctgccg ggtcaccgga tcacgggtaa gaaaattggt
     4801 cgtgatcttc tttggaagaa cgatgagccg ctgaccctcg aagccccggc gtttacggtg
     4861 tgtatgtttt tgaaaaggca tggcgcttgc catgcaatgt aagaaaatca gaaaataatc
     4921 catcttttcc ggaagaaata ctattctttc ttttttaatg catcgcgaaa tttggggcta
     4981 attaaacgat tgcaatgccg aaactaattg tcagtgtctt tttgtgtgca ctgttaaccc
     5041 tctcttcctc cgcccaaccc cccgcggcgc cagccgaaac caccgcgccg gccgccaacg
     5101 ccgcgccgac cttaaacgcg gatcaggtgg taacggtcct gtcgcaggac gggcgaacgg
     5161 aggtccgcat aacaatcggt actaccatcc gctatagcgt cgcctggcaa caccgccagg
     5221 tcatcgacgg ctcccccatc tccttaatag tcgacggcgt cgtactggga cagaacgcag
     5281 tcgtcaaagc catctacagg gacagctccc ggtccgtcat caccccctta tacggtaagt
     5341 tcgcccgctt gacggacgaa taccgccaac tgcggatcga ttttgcccag cctttctcgc
     5401 tcatccttcg ggtgtatgac gagggaatag cctggcgctg gatcacctct ttcccgtccg
     5461 acatcaccgt caacgcagaa gaagtaaacg tcaaactcac aagcacagac agcggattac
     5521 tatcggtaac gccaaagctc accacctggg aactgccgta taccccgtac gcaaccatca
     5581 ccgaagtgcc ggattcgatg aacgtcatcg ttcccgccct gttcaccgac caacaactac
     5641 ggctggtggt cgccgagtct gatctgcgtg attatccggg aatgttcctg aaacacggcc
     5701 ccggcggctt ccacgggtat tgggcagcct atcccaaaaa ggaaacaccc tacggcggcg
     5761 actggggaat gtccacaaag gtcgatgaac gcgaagacta tatcgcccgc acagccggca
     5821 acaggtcctt tccctggcgg gtgatcatcc tggccgatga cgataagcgg ctgctgacca
     5881 gtcagctcat ctacaaattg gcggctcctt ccgtgatccc tgacgtctcc tggatcaagc
     5941 caggcaaggc cgactgggaa tggtggcacg acgcgatcgt gaacgacgcc ggattcccca
     6001 ccggcatgga gaacaggagc acgaaactct atgaacacta tatcgatttc gcggcaacgc
     6061 accacctcga atacaccctc atcgacgcgg gatggagcaa tttctatcat ctcgactctc
     6121 cacaacgcgt tgacataaac caactggtca cctatgctaa ccaacgcaat gtaggcctcc
     6181 tcgtctggtg caacgcgacc tcgctgctgg gcaacctcga tcattacatg gagaccttcg
     6241 ggaaatgcgg gttgaagggc atcaaggttg acttcttcga ccgcgacgac caggagatca
     6301 tccgccaaca ggagcagatt gccgcggccg cggccaaatg gcgcctgctc gtagacttcc
     6361 atggctcccc aaaacctaca ggcctgcaac gcacctatcc caacgtgatc aactatgagg
     6421 cggtaagagg ggaggagtgc agcaagtggg atacgacttc caatccgcaa tatcatcttc
     6481 agatcccgtt catccggatg ctgggcggcg acctggacta tacacccggc tcgatgcgca
     6541 atcgctccaa agcagccttt gtgccgatac ccaatggcct tcctaccacg atgggcaccc
     6601 gctgccacga gctggccctg ttcatcctct tcgaccagta cctcgccgtg ctcagcgact
     6661 cgccggaggc ctaccgcaag tacgccgata tcatggcgtt cctgtcggcc gtcccggtgt
     6721 ccttcgacga tacccggatc ctcgcggcga aactaggcca atacgtcgtc ctcgctaaaa
     6781 gaaagggaac cgactggtac gttggcgcac tcaccaactg gacggggcgc gacgtcacac
     6841 tggacctgtc gtttctggat cagggcaact acaaagcgga tatcctgaaa gatggcgccg
     6901 acgccggcgc caacgcagaa tcttacacaa acaaaaaaac aactgtcgat caacacaccc
     6961 aactgcatct gcacctggct tcgggcggcg gagcggtaat aaaaatctca aaagcttcca
     7021 tatgacaaag aaccttttac tgctactgct tggcgtatgc actattttag tccatgcgca
     7081 gggtcagacg cccggcaacg gaggaagtct caccatccgg ggaaccgtcc gggaaagaaa
     7141 ttccgggcag gccctcgccg gcgtacagat ctctcagaaa acggatccga agaatgtggt
     7201 cgtcgccgga gcggccggcg aatttacgat cacggtgccg gcgctccata ccatcctggt
     7261 ctttcacgcc atgggctaca ccgacctgga gatggatgtg gccggccgta cttcgctgac
     7321 ggtcgacctc gagtcgggcg gcgccggcaa tctcgacgag atcatcgtcg tcggagccgt
     7381 ggtcaaacga catgacctga caggttcggt cgctaccgtc gacgccaaaa ccatcggcat
     7441 cacccctacc tcggatatca atacggcgat ccagggaaag gtcgccggcg cctacgtcgt
     7501 caacagcgca caaccgggca cggcggcctc catcaggatc aggggcaaca actcgctgag
     7561 ctacggcacc agccccatct atgtggtgga cggggtgatc gtcggtgata acctgaactc
     7621 gctgaacccc gatgacattg ctgacatgga agtgctgaag gacgcttccg caacagccat
     7681 ttacgggtca cgcggcgcca acggcgtcgt actggtcagt accaaaaaag gacggaagaa
     7741 taccggcgag gtccattaca acagttgggt cagctggtcg gaattcgcgc ggaaaatgaa
     7801 attcatgaac gcccagcaga tctacaacct ccgcgccgac gccttcgcca accagtacat
     7861 ggacgacaat cccggcgccg accgccaggc ttatctgaac cgcatcacga gcgatacctc
     7921 caatctcgtg tttgccgact atgaaaagca gacgatcaag tcggggagat cctatgactg
     7981 gctgcacgcc gtctcgcaga acggaatggc ccagaaccat accctggact tttccggcgg
     8041 atcggacaaa ggcacctatt tcctaagcct gaactacacc gaccagaagg ggttgatcga
     8101 tggcgcggac tacaaacgga tgggtggccg gatcaacatc gacgagaacg taaaatcctg
     8161 gttgaaggtg ggcacctaca ccacgttcac ccacagcatc accgatgtcg cggacgccag
     8221 cgtatttcca acggcagtgg ccgccaatcc gctcttgccg gtagacacca ccggcctgta
     8281 tacgttgaaa tggggggata tcttcaacca ggatcagtac aatcccctgc gtagcctgta
     8341 tatccaaaac aatcaatacc tgaaccggct gctgaccagc acctatatca gcgtatcgcc
     8401 catcaaggac ctcgccatcc gaacctcttt ttccgccgat gtcaccaacg aacagcaata
     8461 cacctatatt cctaccacca cgggccagga caaaaggaac agcaaccagg gcaacgcggt
     8521 gcactacaag gtggaaaact ataatctcca gtgggataat tccgccacct atgcgaagac
     8581 gtttggcgag catcacctga atttcctggt cgctacctcg atgcaggaaa acaggtccag
     8641 ctacaaccag ataacggctt acggcttccc caatgacgac ttcgggtata tgtacctggg
     8701 tggtgtcacc tcgagggaca gaacggtgct ggcgtcggat tttgtcacca caacgctgat
     8761 gtcctatatg ggcagggccg aatggaactg gaagaataca tactatgcca cagccactct
     8821 ccggtacgac ggctcatcca agttcggtcc gagccaccag tggggcgcct ttccttccct
     8881 ggccgtcgcc tgggacatga gccgggaaca atttatgtca aaattcagcg tcatcagcca
     8941 ttggaaatgg cgggttggct atggcgtggc cggaaaccag aatatcccgg tgtactccac
     9001 ttattcgctg taccggcctt cctatacaaa cgggacggtc acctatgtaa gcgacggaag
     9061 actgggcaat ccggatctgc aatgggaaag acaaaaacag gtcaacgtcg ggacggatat
     9121 cggcctgctg aacgaccggc tcaatcttac ggccgactac ttcaatataa agaacgacaa
     9181 cctgctcatc gacgaaagcc tggcccctac ctcgggtttt accaataagg tcgccaatgt
     9241 cggcgccatg acgaacaaag gcatcgaatt cacgctggac tatgccatcg taaaacacaa
     9301 agacctgcaa tggaatttcc ggttcat
//
LOCUS       DATGFG010000894         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_860182, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000894 DATGFG010000000
VERSION     DATGFG010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860182"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VKQ52_16870"
     CDS             complement(<1..394)
                     /locus_tag="VKQ52_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88932.1"
                     /translation="MDTLDDELVRFWKTLNENSVRYIMVGGFAIRFHGFNRSTDDLDM
                     WLEDTIENRKHLRKAFSDLGYGDFISLETMQFVPGWTNFYIGGGIELDIMTNMKGLEN
                     VSFGECLEMASIADLEGIKVPFLHINHLI"
     gene            complement(405..578)
                     /locus_tag="VKQ52_16875"
     CDS             complement(405..578)
                     /locus_tag="VKQ52_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88933.1"
                     /translation="MVEKNKGSGENVAPPLDDAEKERIKRDMYRSDIEKLKLFTRMLR
                     QNVLFKKAKIIHK"
     gene            819..1715
                     /locus_tag="VKQ52_16880"
     CDS             819..1715
                     /locus_tag="VKQ52_16880"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ88934.1"
                     /translation="MKVFVTGATGFIGSALVHELLHAGHQVLGLARSDASAGALLAAG
                     AQVHRGDLQDLDSIRTGTAASDGVIHAGFIHDFSRFKEVCEVDRLAIETIGATLAGSN
                     RPFIVTSGTALVRQGSLATEDMASNHDRTQFPRVSETAALAFTTKGVRSSAVRLSPSV
                     HGEGDHHGFIPILIGIALQKGVVAYIGDGHHRWNAVHRLDAVHLYRLALEKGEAGVRY
                     HAVGDEAITLKAISEAIGRQLKLPVVSLSPEEAAAHFGWFAQMAALDCPASSKITQER
                     LDWHPSHPGLLEDLAGDIYFKP"
     gene            1728..2645
                     /locus_tag="VKQ52_16885"
     CDS             1728..2645
                     /locus_tag="VKQ52_16885"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ88935.1"
                     /translation="MNKQLTRIKTISEFHRLADLPKPEHPLITVIDLETLKRPPIEGT
                     YTRVFDFYTISLKKNFHVKMRYGQQPHDFDEGILHFMAPGQVLTVEFEKGQVHSPSGW
                     MILFHPDLLLNTPLAKTIKQYEYFGYAANEALFLSDKEETMITGIAKNMEQEYHANID
                     RFSQSVIIAQLELLLTYGDRFYQRQFITRKVASQEILSRLEDLLNTYFTSGALAKQGL
                     PTVHYIAEALHLSPNYLSGLLKTLTGQSTQQHLHDKLIGLAKEKLSTTAMSVSEIAYE
                     LGFGHLASFSKLFKKKTNLSPLEFRQSFN"
     gene            complement(2660..3334)
                     /locus_tag="VKQ52_16890"
     CDS             complement(2660..3334)
                     /locus_tag="VKQ52_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88936.1"
                     /translation="MCFITRIARIILPVILVIVFTGCRKDQAASQPSLSSQIQKIESA
                     FKGWVVIPDPSVTSMSDAQVDAIMAFIKTVSGRKPVVAGTIVEAPGAKVQVTGSDTYL
                     LDFGAVYDGYTVNASATIKYATASTGNNVISGASGISSQGSAQTRTVPYEGSNAQLTQ
                     TWTPQLGSVQSFAADGSQITLQWGGQVNYSYNYNGTDIKAPSQGFFFAQTVGASSLPT
                     DPIQPP"
     gene            3707..>4003
                     /locus_tag="VKQ52_16895"
     CDS             3707..>4003
                     /locus_tag="VKQ52_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88937.1"
                     /translation="MLRSYLRIAWRNLRKNKGYSFINIFGLATGLAITLLIGLWIADE
                     LSFDSYHPNHRRIAEIMLRQQVNGSAFGKHRGPGETEIYIGPTISTVLGPTLSTG"
BASE COUNT         1014 a         1152 c          923 g          914 t
ORIGIN      
        1 caataaggtg gttaatatgc aagaaaggaa ctttaatacc ctcaaggtca gcaatagaag
       61 ccatttccag acattcacca aacgagacgt tttccaggcc tttcatattt gtcattatat
      121 ccaattcaat cccaccacct atatagaagt tcgtccaccc cggcacgaat tgcattgttt
      181 ctaacgagat aaagtctccg tatcctaaat ctgaaaatgc ctttcttaaa tgcttccggt
      241 tctcaatagt atcttccagc cacatgtcca gatcatcggt gcttctgtta aacccatgaa
      301 atcggatcgc aaagccgcca accattatat acctaacact gttttcatta agcgttttcc
      361 aaaaccgaac cagctcgtca tccaacgtat ccatagcctc ttatttattt atggatgatt
      421 ttggccttct taaataaaac gttttgtctt aacatccggg taaagagctt cagcttttca
      481 atatcagaac gatacatgtc acgtttgatc cgttctttct ccgcatcatc caggggtggc
      541 gcgacatttt cgcctgatcc tttatttttt tcaaccataa gaagcgcgat ttaagctcgt
      601 tgcaataacg caatttaaac aaaatctgct atactattcg acgtaaaaag gcgaaaaaca
      661 actaatacaa agccaatatt taaacgccaa tcaacccgtg atcaaatcca gcgaaagccg
      721 attccaccgc ctcatttttg gcaaaatcaa aaatagattt ggataaatac ttgtctccct
      781 cccgggcgac atttgtgtaa caaaaaaaca caaaatttat gaaagtgttt gttacaggcg
      841 ccacaggatt tatcggatcc gcactagtcc acgaattact acatgccggt caccaggttc
      901 tggggctggc ccgttcggat gcctcggccg gcgcattact ggcagccggc gcgcaagttc
      961 atcgcggaga ccttcaggat ctggacagca tacgcaccgg aacagccgct tcagatgggg
     1021 tgatccatgc cgggtttatc catgatttct cccgatttaa ggaggtgtgc gaggtggaca
     1081 ggctcgcgat agagaccatc ggcgccaccc tggccggctc caaccggccg ttcattgtta
     1141 cttcgggaac ggcgctcgtc aggcagggca gcctggcaac agaggatatg gcaagtaacc
     1201 acgatcgtac ccaatttcct cgggtttcag aaaccgccgc gctggccttt accacgaagg
     1261 gtgtgcgcag ctcagccgtt cggctatcac cctcggttca cggcgaaggc gatcatcacg
     1321 gttttatccc gatactcatc gggattgccc tccaaaaggg agttgtcgct tatatcggag
     1381 atggccacca tcggtggaat gccgtacacc ggctcgatgc cgtccatttg taccggctgg
     1441 cactggagaa aggggaggca ggagtgcgat atcatgccgt cggggatgaa gccataaccc
     1501 ttaaagcgat ctccgaagct atcggccggc agttgaagct tcccgtggta tccctatctc
     1561 ccgaagaagc ggctgcccac tttggctggt tcgctcaaat ggccgcatta gattgtccgg
     1621 cgtccagcaa aataacgcag gaacgcctcg actggcatcc gtcgcacccg ggcctgctcg
     1681 aagacctggc cggcgatatc tactttaagc cctaattttg tgctgtcatg aacaagcaac
     1741 tcacccggat caagaccatc agcgagtttc accggctggc agacctgccc aagccggagc
     1801 atccgctcat caccgtgatc gatctcgaaa cgctgaaacg gccgccgata gaaggcacat
     1861 ataccagggt ctttgatttt tataccatct ccctgaagaa aaactttcat gtgaagatgc
     1921 ggtatggaca gcagccgcat gatttcgacg aaggtatcct gcattttatg gcgcccgggc
     1981 aggtgctgac cgtggagttc gaaaaaggcc aggtacacag cccttccgga tggatgatcc
     2041 tcttccatcc ggacctttta ttgaacacac cgctggccaa aacgatcaaa cagtatgagt
     2101 atttcgggta cgctgccaac gaggccttgt ttttgtcgga taaggaagaa acgatgatca
     2161 ccggcatcgc caaaaacatg gagcaggagt atcacgccaa tatcgaccgg ttcagccaaa
     2221 gcgtgatcat cgcccagctg gaattactcc tcacgtatgg cgaccggttc tatcagcgcc
     2281 agttcatcac gcgaaaggta gccagccagg agatcctgag ccggttggaa gacctgctca
     2341 atacctattt tacaagcgga gcattggcga aacagggttt acctactgtc cattacattg
     2401 ccgaagcctt acacctgtca ccgaactatc taagcggctt gctgaaaacg ctcaccggcc
     2461 agagtacaca gcagcacctg catgataaac tgatcgggct ggctaaggag aaactctcca
     2521 ccacggctat gtcggtcagc gaaattgcct acgaattggg tttcgggcac ctggcgtcct
     2581 tcagtaaatt attcaagaaa aagacaaacc tatcgccgct ggaattcagg caatcgttca
     2641 attgaagccc ccctccccct cacggaggtt ggatcggatc tgtcggcagt gaactcgccc
     2701 ccaccgtctg cgcaaaaaag aaaccctgcg acggcgcctt gatgtcggtc ccattatagt
     2761 tatagctgta gttgacctgc ccgccccatt gaagggtgat ctgcgagcca tccgctgcaa
     2821 aactttgtac gctgccgagc tgtggcgtcc aggtctgcgt taattgcgca ttggatcctt
     2881 catagggaac ggtccgggtc tgcgcggacc cttgtgaact gatacccgag gctccgctaa
     2941 tcacattgtt tccggttgag gcggtggcat atttgatcgt cgcacttgca ttcacggtat
     3001 atccatcgta caccgcacca aaatcaagaa gatacgtatc gctaccggtt acctgcactt
     3061 ttgcgccggg cgcttcgacg atggtcccgg ccactaccgg tttccgcccg gaaacggttt
     3121 ttataaacgc catgatggcg tccacctgtg catcggacat actcgtgacg cttggatccg
     3181 gaatgacgac ccatcccttg aacgcggact cgattttttg tatctggctg gaaaggctgg
     3241 gctggctggc ggcttggtct ttacgacagc cggtaaaaac aataaccagg ataacaggta
     3301 aaatgatccg ggcgattcgg gtgataaagc acattggata aagtttacgt tttgatggat
     3361 ccgggcctgg cccgcgagaa tgaaggtaat aaacgcgatc ctcccgccct atacatgcaa
     3421 acgcgtaaat ttgatttccc aaacgaacac ctcctgtatc ataaccgaac agccccacct
     3481 gccatcaccc cctcaaacaa ctgaccccca acatcctacc aatccggcac tatcttcgcg
     3541 aacccaacta acggaaaagc caaacctcca tccaacacgg cccgatgggg gcccagctgt
     3601 aaaacccatc ccaacatcct tgcgtcgcac ccttcaacgg caatgcaaca ttgggcaaat
     3661 tccgatgtcc ttaccgacgg aaagcaactc atccaaccaa aataccatgc tccgatctta
     3721 tctccggatt gcctggcgca atctccgcaa gaacaagggc tactccttca tcaatatctt
     3781 cggtctcgcc accggactgg ccatcaccct cctcatcggc ctctggatcg ccgacgagct
     3841 cagcttcgac agctatcacc ccaaccaccg gcgcatcgcc gagatcatgc tccggcaaca
     3901 ggtcaacggg tccgccttcg gcaaacaccg gggccccggc gaaaccgaga tctatatcgg
     3961 ccccaccatc tctaccgtcc tcgggcccac gctgagcacc ggc
//
LOCUS       DATGFG010000895         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_48302, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000895 DATGFG010000000
VERSION     DATGFG010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48302"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VKQ52_16900"
     CDS             <1..299
                     /locus_tag="VKQ52_16900"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HLZ88938.1"
                     /translation="QVQPVGWWLASHIKDGKRHLNLNTFAGLRVAMQKKRMEDCIEQI
                     DSAEMPLSSYTLIHTDAKLSAGEKETLKSWCQHIIDTLKASYPPDSLVLPKRKR"
     gene            complement(485..793)
                     /locus_tag="VKQ52_16905"
     CDS             complement(485..793)
                     /locus_tag="VKQ52_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88939.1"
                     /translation="MSMLQPDKRYEIIKPMLNEGKIQLFQDIFQFQKKTVVAKDLGKR
                     GPRFDYLINRVEKFTVEELLIIAKKCRLSFDEIMQLVKAQLEARAKKGAKTQAKPSKQ
                     "
     gene            complement(1034..1453)
                     /gene="tsaA"
                     /locus_tag="VKQ52_16910"
     CDS             complement(1034..1453)
                     /gene="tsaA"
                     /locus_tag="VKQ52_16910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HLZ88940.1"
                     /translation="MSQSENFCLEPIGYIQSVIHTRAEAPKQGYEGAPDAWLEVAPAF
                     AAALDGIEKGDHLILLTWFHRSKRDVLAVHPRGDANNPLTGVFATRSPDRPNPLGLHR
                     VCVREVVGNRLKIGPIEALDGTPVVDIKPVLSGSADH"
     gene            complement(1495..>2774)
                     /locus_tag="VKQ52_16915"
     CDS             complement(1495..>2774)
                     /locus_tag="VKQ52_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLZ88941.1"
                     /translation="LPMDKRSFLKGLALTGAGLPLTLSGMAEWIGRFDRQPATTLAGN
                     EEFWTGIRSGYRLKPDYINLENGYYNILPEQTLEAFIRHVREVNYEGAYYMRTVQFDR
                     KKAAAARLATLAGCSPDELVITRKTTESLDMIIGGYPWKEGDEAIMALHDYGAMLQMF
                     RQVEKRHGIVRKVVSIPNHPASDDELVTLYEKAITPRTRLLMVSHMVNITGQIMPVRK
                     ICEMAHARGVEVMVDGAHSFAHIRHSIPDLACDYYGASLHKWLSVPLGAGILYVKKDL
                     TGKIWPLLAEPDAGAQGIARLNHTGTLPVHTDLAINDAIDYYDTLGHEKKEERLRYLQ
                     QYWTSKVRNLPNVVVNTPAEPERGCAIANVGIRGIKPADLASRLLKQYKIYTVAIDLP
                     EGNVQGCRITPNIFTLPEELDTLVAAVKAMSAV"
BASE COUNT          551 a          733 c          834 g          656 t
ORIGIN      
        1 cccaggttca gccggtgggg tggtggctgg ccagccatat caaggatggt aagcgccatc
       61 tcaacctgaa tactttcgcc ggtctgcgcg tggcgatgca gaagaaaagg atggaagatt
      121 gtatcgaaca gatcgatagt gcggaaatgc cgctttcttc ctataccctg atccacaccg
      181 atgcgaagct tagcgccggc gagaaggaaa cgctgaagtc ctggtgccag catattatag
      241 ataccctcaa ggccagttac ccgccggaca gcctggtgct gccgaagcgg aagagataag
      301 ccagcgggcg acggggccac gcgccgaagc ggaagaaata agacggtgac ggggcactcc
      361 cgcggcgccg cataaaaaaa ccgtctcttc gatgagacgg cttgcgttga ggtctgggta
      421 tggtctaagc tcggataatt ggatcggcga taggcaaggt atggctatgg taatcaggac
      481 aacattattg tttggagggt ttggcctgcg tcttcgcacc ttttttggcg cgggcttcca
      541 attgagcttt taccagttgc atgatctcgt caaacgataa gcggcatttt ttggcgatga
      601 tcaacagctc ttcgacggtg aatttctcta cacgattgat caggtagtcg aagcgcggcc
      661 cacgttttcc cagatccttc gcgacgaccg tctttttctg aaactggaaa atatcctgga
      721 acagttggat ctttccttca ttcagcatag gtttaatgat ctcataccgc ttgtccggct
      781 gtaacatact caaagaaaag atttaatatt tgccaatggc cggttttgca tttttttcac
      841 tttttacggg aagattcttc acttttttag tgcgtttttt cactttctga ctgccgattg
      901 ttcacttttt gccggcatat ttgtcctttt tacgatccca tatcgatggc gcgccccagg
      961 ctccgggcct gccgcgtgcc ccggtgcgaa ttttgaggcc gccggcgctg gtgatcggtc
     1021 gactcgcccg gcgctaatga tccgccgacc cgctcagcac gggtttgata tcgaccacgg
     1081 gcgtcccatc caaggcttcg atggggccga tcttcagccg gttacccact acttcgcgta
     1141 cgcatacccg atggagaccc aggggattgg gccggtcggg ggatcgggtg gcgaatacgc
     1201 cggtgagagg attgttggca tcgccgcgtg gatggacggc gaggacatcg cgtttcgatc
     1261 tgtgaaacca tgtgagcagg ataaggtgat cgcccttttc gatgccgtcg agtgccgcgg
     1321 caaaggccgg agcgacctcg agccaggcat cgggcgcgcc ttcatagcct tgttttggcg
     1381 cttcggcacg ggtatgaata acggattgga tataaccgat cggctcaaga caaaaatttt
     1441 cggattggct catatggggc tatttcgaca aaggtagcgc taaccccgat agcgttacac
     1501 cgcggacatc gctttcaccg ccgccaccag cgtatccaat tcctccggca gggtgaagat
     1561 attcggcgtg atacggcagc cttgtacgtt gccttccggc agatcgatgg cgactgtgta
     1621 gatcttgtac tgtttcaaga ggcgggaggc caggtcggcg ggtttgattc ccctgatccc
     1681 cacgttggcg atggcgcagc cgcgttcggg ttccgcgggc gtattgacca cgacattggg
     1741 gaggttgcgc actttcgagg tccaatattg ttgcagatag cgcaggcgct cctctttctt
     1801 ctcatgcccc agcgtatcat aatagtcgat cgcgtcgttg atggccaggt cggtgtgcac
     1861 gggtagggtc cccgtatgat tcagccgcgc aatgccctgg gcgcccgcat cgggttcggc
     1921 cagcaggggc cagatcttgc ctgtgaggtc cttcttgaca tagaggatgc ccgcacccag
     1981 cggaacgctg agccatttgt ggaggctggc gccatagtaa tcgcacgcga gatcgggtat
     2041 cgaatgccgg atatgtgcaa atgaatgcgc cccatccacc atcacctcca cgcccctggc
     2101 atgcgccatt tcgcagatct tgcgcacagg cattatctgg ccggttatgt tgaccatatg
     2161 gctcaccatc agcaaccgcg tgcgcggcgt gatcgccttt tcataaagcg tcacgagttc
     2221 gtcgtccgag gcgggatggt tgggaatgga gacgaccttc cggacaatgc catgccgctt
     2281 ttccacctgg cggaacatct ggagcatggc gccgtagtca tgcagggcca tgatggcttc
     2341 gtcgccttct ttccagggat agccgccgat gatcatgtcg agggattcgg tggttttacg
     2401 ggtgatgacc agttcatcgg gcgagcagcc cgcgagggtg gccagcctgg cggcggcagc
     2461 ttttttgcga tcgaattgaa cggtacgcat ataataagct ccctcgtaat tgacctcgcg
     2521 tacgtgccgg atgaaggcct cgagggtttg ttcggggagg atattgtagt agccgttctc
     2581 gagattgata tagtcgggct ttagccggta gccgctacgg atgccggtcc agaattcctc
     2641 attgccggcg agggtggtcg cgggttgccg gtcgaagcgg ccgatccatt cggccatgcc
     2701 ggagagcgta agggggaggc cggcgccggt gagggccagt cccttcagaa agctgcgctt
     2761 gtccatgggg agaa
//
LOCUS       DATGFG010000896         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1213285, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000896 DATGFG010000000
VERSION     DATGFG010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213285"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..512
                     /locus_tag="VKQ52_16920"
     CDS             141..512
                     /locus_tag="VKQ52_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88942.1"
                     /translation="MNNATLSRIGTIFYALVIGFFGINHFLYGTGMQKMVPAFLPGGV
                     IWVYITGGCLLLAAIAFLIDKQVRLAALLLALFLLLIVLSVHLPAVMHAPDANARRFP
                     LTNLIKDTGLAAAALLVAARK"
     gene            complement(656..1480)
                     /locus_tag="VKQ52_16925"
     CDS             complement(656..1480)
                     /locus_tag="VKQ52_16925"
                     /inference="COORDINATES: protein motif:HMM:NF006133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ88943.1"
                     /translation="MKNFENKTVLITGGTRGIGKAIALRLAAEGANIAIVGKTAEPNP
                     KLEGTIYTAAEEIAAAGGGRVLPLQGDIRFEDKIRSIVDQTVATFGGIDILVNNASAI
                     NLAATEQVEPKRFDLMHAVNVRGTFFMSQACIPHLKQAHNPHILNLSPPLNLDNRWFA
                     PHLAYTMSKYGMSMIVLGLAEELKQYRIAANALWPRTTIATAAVKNLLGGDFLIQRSR
                     TPEIVADAAFYILQKPSFETTGNFFIDEDVLQQEGITDLGKYAINPDQKLMNDLFL"
     gene            complement(2161..3714)
                     /locus_tag="VKQ52_16930"
     CDS             complement(2161..3714)
                     /locus_tag="VKQ52_16930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HLZ88944.1"
                     /translation="MAEYGFRRWIITFTVITAALLELIDTTIVNVALPNIMGNLGATL
                     EDANWIVTGYAVANVIILPMSGWLGDRFGRKNYFLASILIFTIASFLCGNAGSLTELI
                     AFRLLQGLAGGGLISTGQAILLETWPPSEVGMATALFGLGAVVGPTVGPTIGGYITDA
                     PGLSWKWIFYVNIPVGAIAAFCVSTFVRESPRYGQGKRIDWWGILFLAIGVGALQVVL
                     EKGEDKDWTATWWITALIWTTVISAVCFVWREVSIDYPVVNFKILRHRSFFIGIFTSF
                     VLGVALYGSTFIFPVFCQNLLGFTAQQTGLILFPGGLATILCMPFVGNMLKKGVPAQF
                     LSAGGFVLFFIFCWMLSSATLESGTGDFFWPLIVRGVGMAILFVPLTTLAISDLKGPE
                     IGQGTGLNNMMRQLGGSFGIAIMTTIIHIRMGTNRAIMLENIHNFNPAFTARYDQMVQ
                     AFLAKGVPLSQAQQMAYAAIEGVVTRQTMLTTYDGMYLLIGVFTLCCVPLIFFQPFKK
                     AAPMPVDAH"
     gene            complement(3755..4801)
                     /locus_tag="VKQ52_16935"
     CDS             complement(3755..4801)
                     /locus_tag="VKQ52_16935"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HLZ88945.1"
                     /translation="MEQNNTQKKPRKKLVFPIILGIVLVGALFFSIKEYIFLQSHEET
                     DDAQVDGDISPVIARVSGYVMEIRFKDNQYVHAGDTLVILDDRDYKIKLDQAIAAQAA
                     AQKNAAAQGAAITEAQSSFAVQKANIEQAQVRLWKATQDYERYKNLYDDHAITKAQLD
                     EVTADRDAAQAGLDAAKSQVPVINTRVNTSRVQTVATSSVIDTRKADVEYARLQLTYT
                     VITAPASGTVSKRNIQVGQLVQAGSPLFSVVHDDVYITANFKETQMRDIALNQKVDIR
                     VDAFDKQVISGTIESFSGATGAKFSLLPPDNATGNFVKVVQRIPVRIHIDADSSVLRR
                     IRPGMSVDVIVHTK"
     gene            complement(4845..>5732)
                     /locus_tag="VKQ52_16940"
     CDS             complement(4845..>5732)
                     /locus_tag="VKQ52_16940"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ88946.1"
                     /translation="YLVISSYLNYDKILENQLIVAQNMQDVQGKLEEITKYESQGLAT
                     QNDVLRYQLQKSQMQLTEIELENNRKIANYDMNIILGLPDTTTIILPPVNYRVSEQLV
                     FSDLLQTAEANRRELQDLSYQTRIADINVRKMHDQRLPTVAASGGMYYINPTGDVIPT
                     HNNLIAPLTLGIGVSWDISTLYTNKNKEREVAVQRRELNTAHDQAIDDIRQDVHRQYI
                     LYQNALEKIKVLEVAVAQAQENERITESKYRNNLVNTTDRIDAQTLLYQARVNLELAK
                     SDATISYYDILRATGKMHI"
BASE COUNT         1348 a         1566 c         1464 g         1354 t
ORIGIN      
        1 atgtgctctt tttcccgcaa atgaggccag agaaggctcc cgagagccaa actaccggca
       61 acgcttagcc ttgtgcaaaa atcgttcatg gttatctgat aattttttgt tatattggat
      121 gtaactaaaa cgattgtcat atgaataatg caaccctctc ccggataggt acgatcttct
      181 atgccctcgt tattggtttc ttcggtatca atcactttct gtatggtacc ggcatgcaga
      241 aaatggtacc ggccttcctt cccggcggtg tcatctgggt atatataacg ggcggttgcc
      301 tccttctggc agcgatcgcc ttcctcatcg acaaacaggt ccgactggcc gcccttttac
      361 tggccctttt cctattactg atcgtattgt cggtccacct tccggccgtc atgcacgcac
      421 cggacgccaa tgcccggcgg tttcccctga ccaacctgat caaggacacg ggtctcgccg
      481 ccgccgcatt attagtggcc gcacgaaaat agtcacggcg atcccggcgg accaatgcaa
      541 accacggtcg gccgatggcc ccctataaaa aaatacctcc cgcactaagc gggaggacgg
      601 ttgctttttt ttacccaagg attaaaatga aaaagaatta tgcctctatt atctgttaaa
      661 ggaacaggtc attcatgagt ttttgatcgg gattgatggc gtacttgcca agatcggtga
      721 tcccttcctg ttgaagaacg tcctcatcaa taaaaaaatt gcccgtcgtc tcaaaagaag
      781 gtttttgcaa aatataaaag gccgcatccg cgacgatctc gggcgtgcga ctgcgctgga
      841 taagaaaatc gccgccgagc agatttttga cggctgcggt ggcgatcgtc gtcctgggcc
      901 aaagggcatt tgccgctatc cggtactgct tcagttcctc ggcaagcccc agcacgatca
      961 tgctcatgcc atacttgctc atcgtgtagg caagatgggg agcaaaccag cggttatcca
     1021 ggttgagggg tggtgaaaga ttgaggatat gagggttatg cgcctgctta agatgcggga
     1081 tacaggcctg gctcatgaag aaggtaccac gtacattgac cgcatgcatc aggtcgaagc
     1141 gcttgggctc cacctgttcg gttgcggcaa gattgatcgc gctcgcatta ttgaccagga
     1201 tatcgatccc gccaaaggtg gctaccgtct ggtcgacgat cgaccggatc ttgtcctcga
     1261 agcggatatc tccctgtaag ggaagaacac ggccgccccc cgcggcggcg atctcctcgg
     1321 ccgcggtata aatggttccc tccagcttgg gattgggctc ggcggtcttc cccacaatgg
     1381 cgatattggc gccttccgcg gccagccgca gggcgatggc cttgccgatg ccccgggtgc
     1441 cgccggtgat caggaccgtt ttattctcga aattcttcat gaatcttgac tttgtccgcc
     1501 ccggcaggcc ctgacgggaa attagccccg ccgggccgga ccattaataa ttgccttcgg
     1561 cgaacgggct ttcagcccgt ttggtcatca ttatcaacta caaaataggg taatttatga
     1621 tgtagccaaa aaaatagcgg gccaatacca aaaccgtatt cgtaattcta cgaacccaac
     1681 cgtaaattca acaacagaga aatatagcat ttattctctt gcactgatta acaccctatt
     1741 gtatgtttgt attgttaagt ctgattgata agcgattcgc gatccaatgt tccttgaaga
     1801 aagccaactg aatgaatgaa tgaaaaaatc caaagaaaaa ccaaaatcca atttctgaaa
     1861 gaaaaccaag atctgaaatc cttacaaaaa ccgaaagaaa tccaacaatc cggaaaaacc
     1921 cagaatcctt aatcgatcaa aacttattcg caaatccaaa atccaagttg atccagatcc
     1981 taagaacaag ccgaattaaa atttgtacct attgatggcc gtgaaactgt tggttaagta
     2041 tcgtacaaaa aaattgaaaa gtcctgaata aattcaggac ttttcttttt gcatggactg
     2101 atgtctgagc tgttggttag gttgcctttc cgttgtgtct ttttcctatc ttaaaaagtt
     2161 ctaatgtgcg tcgacgggca tcggggccgc tttcttaaag ggttgaaaga agatcagcgg
     2221 tacgcaacag agcgtaaata caccgatcag caggtacatc ccgtcatagg tggtgagcat
     2281 cgtttgccgg gtaacaacac cctcgatagc ggcataagcc atctgctggg cctggctcag
     2341 gggcacaccc ttcgcgagga aggcctggac catctgatca taccgcgccg taaaggccgg
     2401 gttgaagttg tggatattct ccagcatgat ggcccggttg gtccccatcc ggatatgaat
     2461 gatcgtggtc atgatggcga tgccgaatga gccgcccagc tgccgcatca tattgttcaa
     2521 ccctgttccc tggccgatct cggggccctt caggtcggaa atcgccagcg tggtcagcgg
     2581 tacgaagagg atcgccatcc cgacaccccg gacgatcagc ggccagaaga aatcgcccgt
     2641 tccggattcc agcgtggcgc ttgacaacat ccagcagaag ataaagaaga gcacgaaacc
     2701 accggcggaa aggaactggg ccgggacacc cttctttagc atattaccca caaacggcat
     2761 acaaaggatg gtggccaggc cgcccggaaa gaggatcagc cccgtctgct gggcggtaaa
     2821 gcccaggagg ttctgacaaa acaccgggaa gataaaggta ctgccatata gcgcgacccc
     2881 cagcacaaaa gaggtgaaga tgccgataaa gaagctgcgg tggcgtaaga tcttgaagtt
     2941 cacgacgggg taatcgatgc tgacctcccg ccagacaaag cacacggccg aaatgaccgt
     3001 cgtccagata agcgccgtta tccaccaggt agccgtccag tccttgtcct cgcctttttc
     3061 cagcactacc tgcaatgcgc ccacgccaat cgccaggaaa aggatgcccc accagtcgat
     3121 gcgtttcccc tgcccatatc ttggcgactc ccggacaaag gtggacacgc aaaaggcggc
     3181 tatcgccccc acggggatgt tgacataaaa gatccatttc caggaaagtc ccggtgcgtc
     3241 cgtgatataa cccccgatgg taggtcctac cgtaggtccc acaacggcgc caagaccaaa
     3301 aagcgccgtc gccatcccta cttcgctggg cggccaggtc tcgagcagga tcgcctgacc
     3361 ggtggagatc aacccgccgc cggccaaccc ctgcagaaga cggaaggcga ttaattcggt
     3421 cagactgccg gcattgccac aaaggaagga ggcgatcgta aagatcagga tcgacgcgag
     3481 aaaataattc ttccggccaa accggtcgcc aagccatccc gacatcggca ggatgatcac
     3541 gttcgccacc gcataccccg taacgatcca gttggcatcc tcgagcgtag cacccaggtt
     3601 acccatgata ttggggagtg ccacattcac gatagtcgta tcgatcagct ccagcaacgc
     3661 ggccgtaata acggtgaaag tgatgatcca ccttctaaaa ccatattctg ccataaaaaa
     3721 agataattaa gatgttcaat cagggcgact ggtcctattt ggtgtggacg atgacatcca
     3781 cgctcatccc aggccggatc cggcgcaata ccgaactgtc ggcgtcgata tggatccgca
     3841 ccggtatccg ctgaacgacc tttacaaagt tacccgtggc attatccggc ggcaggagcg
     3901 agaacttggc gcccgtcgcg ccggagaacg attcgatcgt accggagatc acttgcttgt
     3961 cgaaggcatc tacccggata tccacttttt ggttcagcgc gatatcccgc atctgggtct
     4021 ccttgaagtt ggccgtgata tagacatcgt catgcacgac cgagaaaagc ggcgacccgg
     4081 cctggaccag ctggcccacc tggatattcc gtttggagac ggtcccgctt gccggggccg
     4141 tgatcaccgt ataggtgagc tgcagccggg catattccac atcggccttg cgggtatcga
     4201 tcacggagct ggtggctacc gtctgcaccc ggctggtatt gacccttgta ttgatcaccg
     4261 gaacctggct cttggcggca tccagccctg cctgcgcggc atccctgtcg gcggtcacct
     4321 catccagttg cgcctttgtg atggcatggt cgtcatacag gttcttgtat cgttcgtagt
     4381 cctgggtggc cttccacaaa cgtacctgcg cctgctcgat attcgccttc tgtacggcaa
     4441 agctggactg cgcttccgtg atggcggcgc cctgtgcagc agcattcttt tgggccgctg
     4501 cctgggcggc gatcgcctgg tccagcttga tcttataatc ccggtcgtcc aggatgacga
     4561 gggtgtcccc cgcgtgtaca tattggttgt ccttgaaccg gatctccatc acataaccgg
     4621 atacccttgc gatcacgggg ctgatatccc catccacctg cgcgtcatcc gtttcctcgt
     4681 gcgactgtaa gaatatatac tcttttatag agaagaacag cgctccgacc aggacgatac
     4741 ccaggatgat cgggaaaacg agttttttgc gaggcttttt ttgagtgtta ttctgttcca
     4801 tatactttaa atgatcttgt ttgaattgtt ttggttgata cgatttagat atgcattttt
     4861 cccgtagccc taaggatatc ataataggag atggtggcat cggatttcgc cagctccagg
     4921 ttcacccgcg cctggtacaa taaggtctgg gcatcgatcc ggtccgtggt attcaccagg
     4981 ttgttccggt atttcgactc ggtgatccgc tcattctcct gggcctgggc taccgcaacc
     5041 tccaaaacct tgatcttctc cagcgcattc tggtacagga tatattgacg gtgtacgtcc
     5101 tggcggatat catcgatggc ctggtcgtgg gcagtgttca gctcccttcg ctgtacggct
     5161 acctcccgct ccttgttctt attggtgtac agcgtactga tatcccagga gaccccgatg
     5221 cccagcgtga ggggcgcgat caggttgtta tgcgtcggga tcacatcccc cgtggggttg
     5281 atataataca tcccacccga tgcagccacc gtcggcaaac gctgatcgtg catcttcctg
     5341 acattgatat cggcgatccg ggtctgatag gagaggtcct gcaattcccg gcgattggcc
     5401 tcggccgttt gcaacagatc gctaaagacc agctgctcgc tgacccggta attcacgggc
     5461 ggaaggataa tggtcgtcgt atccggcaga ccaagaatga tgttcatatc gtagttggcg
     5521 atcttccggt tattctccag ttcgatctcc gtcagctgca tctgcgattt ctgtaactgg
     5581 taccgtaaga catcattctg cgtcgcaaga ccctggctct cgtatttcgt gatctcttcc
     5641 agcttgccct gtacatcctg catgttctgc gcaacgatca gctggttctc gaggatctta
     5701 tcatagttga gataggagct gatcacgaga ta
//
LOCUS       DATGFG010000897         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_53390, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000897 DATGFG010000000
VERSION     DATGFG010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53390"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VKQ52_16945"
     CDS             <1..225
                     /locus_tag="VKQ52_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88947.1"
                     /translation="AGYWHHMEYLDSGFAMSLRALQSSFSGKLRGIWYLMGMRNIDTL
                     MKRTAPEWWFNRKKEKTFKAAHKELETLVE"
     gene            complement(910..1944)
                     /locus_tag="VKQ52_16950"
     CDS             complement(910..1944)
                     /locus_tag="VKQ52_16950"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HLZ88948.1"
                     /translation="MSETTSTPPALTSKDFATDQEVRWCPGCGDYSILAQVQKIMPTL
                     GIPRENIVIISGIGCSSRFPYYMNTYGMHSIHGRATAIASGLKATRPELSVWVVTGDG
                     DSLSIGGNHTIHLLRRNFDINILLFNNQIYGLTKGQYSPTSEEGKITKSTPFGSLDHP
                     FNPLALARGADAGFVARSMDRDPKHLQQMLLRSQAHKGASFLEIYQNCNIFNDGAFEI
                     FTEKSSKPLETLFLEQGKPLVFGAQREKGVRLDGFRPVVVELGKEFSESDLWIHDEND
                     FFKAQILVRMFDDPRIEGHLPRPFGVFFENDRPCYEDMLNMQIEEVILSKGKGDLDKL
                     LRGNETWTIA"
     gene            2137..2421
                     /locus_tag="VKQ52_16955"
     CDS             2137..2421
                     /locus_tag="VKQ52_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88949.1"
                     /translation="MKKILMIIGIAGCIAATGCAVDGYVTEQPADVYYERPASPGADY
                     VWIEGDWVWTGGGYRWHNGYWGHPRGGRSWQSGHWDHDGRGYHWNRGHWR"
     gene            2622..3032
                     /locus_tag="VKQ52_16960"
     CDS             2622..3032
                     /locus_tag="VKQ52_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88950.1"
                     /translation="MASDLYTTISLHRFVDELTAGLLPRAVSRKTLIINQIDKDLQVG
                     SDENLLAFILWNLLDKAVESTQNECIHVESVCKGDSTMIRVRNAGVYFYRSCANCFRQ
                     VQDAAEKLGGSISIDSWEMGTTVALTLNKEGWAA"
     gene            complement(3136..3465)
                     /locus_tag="VKQ52_16965"
     CDS             complement(3136..3465)
                     /locus_tag="VKQ52_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLZ88951.1"
                     /translation="MNIYISNLSFNVTDEDLQDYFAEYGEVSSAKVIMDKFTGKSRGF
                     AFVEMPDDEAAKKAIQELDGASVDGRTIGVSVAKPREDRGNGGGNKGGGGSFQRRGNN
                     NYSNSRY"
     gene            complement(3696..4838)
                     /locus_tag="VKQ52_16970"
     CDS             complement(3696..4838)
                     /locus_tag="VKQ52_16970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ88952.1"
                     /translation="MAVCTGLIVANIYYCQPLLVLISRSFGIPESRGGRIAFLTQLGY
                     ALGLLFFVPLGDKVERRGQIVWMAAFAVGALVFAALSPNLLCLEVASVLIGASSVIPQ
                     LILPLAAHLSPPEKTGKVIGTIMSGLLIGILLSRTLSGFVGRWLGWRGMFGVAAGISF
                     SLLLIIRYTFPPSRPQFTGSYASLMRSLLTLAKEQPVLREAAAINALGFATFGMFWTT
                     MVLHLSGAPFHFNSDLIGLFGLAAAAGALAAPLVGGSADKRNPRIAIGYGLGLLLLSF
                     VLLYIGAGYVVGMIAGIILLDLAMQCIHVSNQSRVYALIPQARNRLNTVYMTVSFTGT
                     SLGSAIGLFAWDKAGWVGVCVTGVLLTSAAFTIYALTFKRDRPVAA"
     gene            4993..5403
                     /locus_tag="VKQ52_16975"
     CDS             4993..5403
                     /locus_tag="VKQ52_16975"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLZ88953.1"
                     /translation="MLKYKKIAVITSLLGLMIIGMAAVRPQDDPKPKHNFKVLPKDIS
                     HDDLDKIMDQWKAALGVKCNFCHAPSADSTSHHLDFASDAKPEKNIARHMYKMTGKIN
                     KKYFSFNKDDKGNTIPAISCMTCHRGSPHPGESK"
BASE COUNT         1440 a         1522 c         1339 g         1206 t
ORIGIN      
        1 gccggctact ggcatcatat ggaatacctg gacagcggct ttgccatgag cctccgggca
       61 ctgcagtcct cgttcagcgg gaaactaagg ggcatctggt atctgatggg gatgcgtaac
      121 atcgataccc tgatgaaaag aaccgcgccc gagtggtggt tcaaccggaa aaaggaaaaa
      181 acgttcaagg ctgcccacaa agagcttgaa acccttgttg agtaagggct tacagcgccc
      241 cgggacttcg ggaaagcacc cggccggcag ggggtggaca aaaattaaaa aaaaatattg
      301 ctttttaccg aaactgtctt tatttttacc gcgcttactt actctgatac actcaccgtc
      361 caatttccat ccgaagattc caattccatc gatatccggg ttccatgcct tcttatgaag
      421 cgatcgcttc atagttttga gatacctaac atcccctgtc ctgacgagaa tctcttcgtt
      481 accctgtttg aagcaattcc atccggtatg ttcagtcccc ggctgtctac cgactaatcg
      541 acccgataac ttattgtttt gcggatttgt cttcataaga ctgccgtaaa atctgaccga
      601 tactaacagc ctggtatagt ggagtagaat gtctgtcgaa cagacagttc atgcaacaat
      661 agaatagagc acaatgaaaa atatttcgaa ctttttcttt ttgtggtttc agaggtaagc
      721 acaggccggc gatgtctatc gctggctctt ttttattaat acctccgcgt attgatacct
      781 ctgagcaaga cccgaaaaat gacttcactg tgaaaaaacc aaaaaagagg cggctcaatc
      841 ctgtcgggcc ggcctctttt taattttgta gaggtgctgt actgcgctat gtcctgaggg
      901 atgccgggac tacgcgatcg tccaggtctc attccccctt agcaatttgt ccaggtcgcc
      961 cttccccttg ctaaggataa cttcttctat ctgcatattg agcatgtctt cgtaacaagg
     1021 ccggtcgttc tcgaagaaaa cgccaaaagg cctcggcaga tgaccctcga tacgcgggtc
     1081 atcgaacatc cgcaccagga tctgcgcctt gaagaaatcg ttctcgtcgt gtatccaaag
     1141 atcgctctcg ctgaattcct tccccaactc gacaaccacg ggacgaaaac cgtcgagccg
     1201 gacgcccttc tcgcgctgcg cgccaaagac aagcggtttg ccctgctcga ggaacagcgt
     1261 ttcaagcggc ttgctgcttt tctccgtgaa gatctcaaag gcgccgtcat tgaaaatatt
     1321 gcagttttga tagatctcga gaaaagatgc ccccttgtgg gcctggctgc gcagcaacat
     1381 ctgctgcaga tgtttggggt ccctgtccat gctccgggcc acaaaaccgg catcggcgcc
     1441 cctggcaagc gctaatggat taaagggatg atcgagactg ccgaaaggcg tcgacttcgt
     1501 tattttcccc tcttccgatg taggcgaata ttgccccttc gtcagaccat agatctggtt
     1561 attgaagagc aggatattga tgtcgaaatt ccggcgtaac aggtggatcg tatgattccc
     1621 cccgatactc aggctgtcgc catcgcccgt taccacccat acactcagct ccggccgcgt
     1681 cgccttcaga cccgaggcga tcgccgtagc ccggccatgg atcgaatgca tgccatacgt
     1741 gttcatataa tacgggaacc ggctgctgca gccgataccg gagatgataa cgatattttc
     1801 gcggggaata ccaagcgtcg gcatgatctt ctgcacctgc gcaaggatcg aataatcccc
     1861 gcagccgggg caccaacgca cttcctggtc ggtggcaaaa tccttcgacg tcaatgccgg
     1921 cggggtagat gttgtttcac tcatgatggg gtcccttata atgggatacc tacaaattta
     1981 cgcttttacc ccgtatctgg aatgacaccc tggggaaata ccgccacagg gaacggcccg
     2041 aaagcgggtt ttccggccca aaacgacggt aacccggcat ggcacaggat tggtccggat
     2101 aagggcagaa gcgctcacga ataaaataaa aaaactatga aaaagatact aatgatcata
     2161 ggaatagccg gctgtatagc cgctaccggc tgtgcggtgg acggatacgt caccgaacag
     2221 cccgcggatg tctactacga acggcccgcc tcccccggcg ccgactacgt ctggatcgaa
     2281 ggggactggg tctggaccgg cggcggctac cgctggcata acggttactg gggacaccca
     2341 agaggaggcc gcagctggca gtccggtcac tgggaccacg atggaagagg ctaccactgg
     2401 aaccgcggac actggcgtta aacagacttt aatcatcccc tatatatatc acggaagccc
     2461 gtatgcgtca agctacgggc tttcctggtt ggcttccatg gtcaatctct tagggtttct
     2521 cttattctcc gaaataaatg tgtcacttct acataaagta atgtcccatt gcgttacatt
     2581 tacacggtta aaagcaaccc ccttactgaa atcctgtatc aatggcttct gatttgtata
     2641 ccacgatctc cctgcatcgg tttgtagacg agctgacggc aggactcctt ccccgtgcgg
     2701 taagccgcaa gacgcttatt atcaatcaaa tagacaagga tttacaggtt ggctccgacg
     2761 agaacctgct ggctttcatc ctgtggaatt tactggataa agcagtggaa agcacccaga
     2821 acgaatgcat tcacgtcgag tcggtctgta aaggcgatag cacgatgatc cgcgtgcgca
     2881 acgcaggggt ctacttctat cgcagctgcg ccaactgctt ccgccaggta caggatgccg
     2941 ccgaaaaatt aggcggatcg atcagtatcg actcctggga aatgggtaca accgtggccc
     3001 tcaccctgaa taaggaaggc tgggccgcat aggcccgatc cgccaaaaca cggcgccccc
     3061 cgggacaaac ttaaacttcc caaaaacaaa aaaggcccct ttataagggg cccttaatat
     3121 caacaaagca atggattaat atctgctgtt gctatagttg ttgttgcccc ggcgttggaa
     3181 agaaccgccg ccgcctttgt taccgccgcc attgccgcgg tcttccctgg gcttcgctac
     3241 cgatacaccg atagtccggc cgtctacgga agcgccatcc agttcctgga tcgctttttt
     3301 ggcagcttca tcgtcaggca tctcaacgaa tgcaaaccca cgggatttgc cggtgaattt
     3361 gtccataatg actttagcag aagaaacttc accgtactct gcaaaataat cctgtaagtc
     3421 ttcgtccgtg acgttgaaac ttaagttcga aatgtaaatg ttcatactaa taactggtta
     3481 aaaatgaaaa caaaagacaa tctgagtaaa aaaagcagaa agtaaagacg actaacaatt
     3541 accggggatt tataatagcg gaaatgatca ttcaatactg gtgctgttat cagctcaaat
     3601 ttaacgacgc aaatatagta ctatttaggt taccgtccaa gcaaatccgt tcctgatcgt
     3661 cgcatttagt aatttgttaa cctgtaacgg tccggctacg ccgcaacagg ccggtcccgc
     3721 ttaaaggtaa gtgcgtaaat ggtaaatgcc gcagaagtaa gcaaaacccc cgtaacgcaa
     3781 acgcccaccc atccggcctt gtcccaggcg aaaagcccga tggccgaccc cagcgaggtc
     3841 ccggtgaagc taacggtcat atatacggta ttgagccggt tgcgcgcctg cggtataagg
     3901 gcatagaccc ggctctgatt cgacacatgg atacattgca tcgccaggtc cagcaggatg
     3961 ataccggcga tcatccccac cacatagccg gccccgatat agagcagcac aaacgaaagc
     4021 aatagcaggc caagcccata cccgatggcg atacggggat tccgtttgtc ggccgaccct
     4081 ccgaccagcg gggcggcaag ggcgccggcc gcggccgcga ggccaaaaag cccgatcaga
     4141 tcgctgttga agtggaatgg cgcacccgac aaatgcaata ccatcgtggt ccagaacatg
     4201 ccaaaagtgg caaaacccag tgcattgata gccgcagcct cccgcaggac gggctgttcc
     4261 ttcgccaggg tcagcaggct gcgcatcagc gacgcatacg accccgtaaa ctgtggccgg
     4321 ctgggcggaa aagtatatcg tattataaga agaagcgaaa agctgatgcc cgccgccacc
     4381 ccaaacatcc cccgccagcc cagccaccgc ccgacaaaac cgctgagcgt ccgcgataac
     4441 aggatgccga taagcagacc gctcatgatg gtgccgatca ccttccccgt cttctccggc
     4501 ggagaaagat gtgcggcaag cggaaggatc agctgcggaa tgacagaact cgccccgata
     4561 aggacactgg caacctccag gcaaagcagg ttgggcgaca aagcggcaaa gacaagcgct
     4621 cccacggcaa aggcggccat ccagacgatc tgcccgcggc gctcgacctt gtcgccaagc
     4681 gggacaaaga ataaaagccc aagggcatag cctagttgcg tcaggaaggc gatccgcccg
     4741 cccctgctct cggggatccc aaagctcctg ctgataagga caagaagcgg ctgacagtaa
     4801 tagatattcg ctacgatcag gcccgtacaa accgccatga tcacaatatg accccttttc
     4861 aggtagtcag gtgccgaagg ttgcataatg ggcgcaaagg tacggaccaa cccccgttcg
     4921 gcgtattgtc aaaatattgt caaatgttgg acgaatatcc ctaaattagt gcacccaaaa
     4981 atttccacta ttatgctgaa gtacaaaaaa atcgccgtta ttacctccct tcttggcctg
     5041 atgatcatcg gtatggccgc ggtccgtccc caggacgacc ccaaacccaa acacaacttc
     5101 aaagtgttgc ccaaggatat cagtcacgac gatctggata agataatgga ccagtggaaa
     5161 gccgcccttg gcgtaaaatg taatttctgc cacgccccct ccgccgacag cacctctcac
     5221 catctggatt tcgccagtga cgcaaaaccg gaaaagaaca tcgcccgtca tatgtacaag
     5281 atgaccggta agatcaacaa gaaatatttc agctttaata aggacgataa aggcaatacc
     5341 atccccgcca tttcctgtat gacctgccat cggggaagcc cccatcccgg cgaatccaaa
     5401 taatcaaaaa acccggccga ggccgggttt tttttatctt attgcttctt attgcttcag
     5461 taatttttgg acgtatcgcc cgcttcccgt ctttatctcc acaaaat
//
LOCUS       DATGFG010000898         4198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1328036, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000898 DATGFG010000000
VERSION     DATGFG010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328036"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..701)
                     /locus_tag="VKQ52_16980"
     CDS             complement(66..701)
                     /locus_tag="VKQ52_16980"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HLZ88954.1"
                     /translation="MSKNKKPKHIAVAGNIGAGKTTLTEMLSKHYKWIPQFEDVDHNP
                     YLNDFYEDMPRWSFNLQIFFLNSRLNQLLEIHAGTETVIQDRTIYEDAHIFAPNLHEM
                     GLMSKRDYNNYFQFFQTLKRMVQPPDLLIYLQGSVPTLVAQIQKRGREYEENIRLDYL
                     KRLNDFYNKWIEGYKEGPLLIVDIDKNKFPENEEHLGEIITRIDSQLYGLF"
     gene            complement(752..1762)
                     /gene="trpS"
                     /locus_tag="VKQ52_16985"
     CDS             complement(752..1762)
                     /gene="trpS"
                     /locus_tag="VKQ52_16985"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HLZ88955.1"
                     /translation="MEKKDKKQEIVMSGIRPTGFLHLGNYFGAVRNYVRMQDAYTCFF
                     MVADLHALTTLNETRELKTHVRRVMAENIACGLDPEKVAFYCQSHVPETAELYLFFNM
                     LAYVGELEKTPTFKDKVRLQPQNVNAGLLTYPVLQAADILLHRATLVPVGKDQEQHLE
                     IARNYANRFNQRYGEVFAEPYAFNFGEALAKVPSLDGAGKMSKSENQMATLYLADDDE
                     TIRRKIAKAKTDSGGQPGAPMPESVANLFQLLRLVGGEDVVRQFEADYAAGTIRYGDM
                     KKELAEGMVRFIAPIREKAAAIQNDGAYLRRVMEQGAAKARASARVTIELAKDAIGLN
                     YF"
     gene            1903..2193
                     /gene="gatC"
                     /locus_tag="VKQ52_16990"
     CDS             1903..2193
                     /gene="gatC"
                     /locus_tag="VKQ52_16990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLZ88956.1"
                     /translation="MEVNDALIDNLSNLARLEFNAAEKESIKKDLQRMIQFVEKLGEL
                     DTTGVEPLQHMSPEINVLREDILQGSVSRKEALSSAPQTDGRYFEVPKVIRK"
     gene            2262..2921
                     /locus_tag="VKQ52_16995"
     CDS             2262..2921
                     /locus_tag="VKQ52_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ88957.1"
                     /translation="MGKQALPVLMGVSLDIAKNEYVALMGPSGSGKSTLMNILGCLDT
                     PTSGTYILNGQDVSKMPDDDLADVRNIEIGFVFQQFNLLPRLTAVENVALPLVYAGVP
                     PRQRIERAMAVLEKVSLTDRSHHKPNELSGGQSQRVAIARALVNNPSLILADEPTGNL
                     DTKTSIEIMDIFTSIQAAGNTVVLVTHEEDIANYARRIVRLRDGVIESDRRRIAETIT
                     T"
     gene            2945..3514
                     /locus_tag="VKQ52_17000"
     CDS             2945..3514
                     /locus_tag="VKQ52_17000"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLZ88958.1"
                     /translation="MAIKIYTKTGDKGTTSLIGGTKVSKSSLRIEAYGTIDELNSFIG
                     LLSDRQDQQNLAGIQPLLREIQDRLFTIGSSLACDPEKEPKLKIPDLKPEDIKLLETA
                     IDTMETELPPMKSFLLPGGHPAVSTAHITRCVCRRAERCCVRMQDEGVFVEPLVLQYL
                     NRLSDYLFVLARFIGHRLAVPEIPWKPRV"
     gene            complement(3662..>4198)
                     /locus_tag="VKQ52_17005"
     CDS             complement(3662..>4198)
                     /locus_tag="VKQ52_17005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ88959.1"
                     /translation="KSTLLHILGTLDSPAAGEVMLQDKKIHELKGKPLAAFRNRHIGF
                     VFQFHHLLPEFSALENVCIPGWIAGRKKREVEERALELLNVLGVGHRVENKPGALSGG
                     EQQRVAVARALINNPDIVFADEPTGNLDTANAKELHQLFFDLRDRFQQTFLIVTHNEE
                     LATMSDRVLHMKDGMMAN"
BASE COUNT          963 a         1488 c          896 g          851 t
ORIGIN      
        1 caacctcgcg cgccccaaca tctaacgccg gcccttcgga catccggcct tttgccctct
       61 ccccctcaaa acaacccata cagctgactg tcgatccgcg ttatgatctc ccccaaatgc
      121 tcctcattct ccggaaattt attcttatcg atatccacga tcagcaacgg cccctcttta
      181 tacccctcga tccacttatt gtaaaaatca ttcagccgct ttaaataatc cagccggata
      241 ttctcctcat actcccgccc ccgcttctgg atctgcgcca ccagcgtcgg caccgacccc
      301 tgcagataga tcaacaggtc gggcggctgc accatccgct tcaaggtctg aaagaattga
      361 aagtaattat tgtagtcccg cttgctcatc aaccccatct catgcaggtt gggcgcaaaa
      421 atatgcgcat cctcatagat cgtccggtcc tggatcaccg tctccgtccc cgcatggatc
      481 tccaacagct ggttaagccg gctgttcaga aagaagatct gcaggttgaa cgaccagcgc
      541 ggcatgtcct cataaaaatc attcaaatag gggttatggt ccacatcctc gaactgcggt
      601 atccacttgt agtgcttgct cagcatttcc gtcagcgtcg ttttgccggc gccgatattg
      661 cccgcaaccg cgatgtgttt tggcttcttg ttcttgctca tgttatcagc attcagttag
      721 ttccaccccc cggctccgcc aggcggcgtc ctcaaaaata attcaatcca atggcatcct
      781 tcgccaattc aatggtcacc cgcgcactgg cccgggcctt cgccgccccc tgctccatca
      841 cccgccgcag gtaggcgcca tcgttttgaa tagccgcagc tttctccctt atcggcgcaa
      901 tgaaccggac catcccctcc gccagctcct tcttcatatc cccataccgg atcgtcccgg
      961 cggcataatc agcctcgaat tgccgcacca catcctcccc gcccaccagc cgaagcaact
     1021 ggaacagatt ggccaccgac tccggcatcg gcgccccagg ctgcccccca ctgtcggtct
     1081 tggccttcgc gatcttcctg cggatcgtct catcatcatc ggcaagatac aaggtcgcca
     1141 tctggttctc actcttgctc atcttacccg ccccatcgag actcggcacc ttggccagcg
     1201 cctcgccgaa attgaaggca tagggctctg caaaaacctc gccataccgc tggttgaacc
     1261 ggttggcata gttccgcgct atctccaggt gctgttcctg gtccttaccc accggcacaa
     1321 gcgtagcccg gtgcaacagg atatccgccg cctgcagaac gggatacgtc aacagccccg
     1381 cattcacatt ctgcggctgc aaccgcacct tatccttgaa ggtaggggtc ttttccagct
     1441 cccccacata cgccagcata ttgaagaaca gatacagctc agccgtctcc ggcacatggc
     1501 tctgacagta aaaagccact ttctcaggat cgagcccaca ggcgatattc tccgccatca
     1561 cccggcgcac atgcgtcttc agctcccgcg tctcattcag ggtggtaagc gcatgaagat
     1621 cagcaaccat aaagaaacac gtatacgcat cctgcatccg gacataattg cgcaccgcac
     1681 caaaataatt ccccaggtgg aggaatcccg tcgggcgaat gccgctcatg acgatttctt
     1741 gctttttgtc ctttttttcc ataattgtgt gccttcgacg cgctttttgc cgatggtccc
     1801 gcgccgccgg cagaccgcta atgtccgccc tgccgggcga cgccgactac gtcgactttg
     1861 acggaagcgc gaagatagct atttttgtcc caaagcactt gcatggaagt caacgacgca
     1921 ctgatcgaca atctgtccaa tctcgcccgc ctggaattca acgcagccga aaaagaatcc
     1981 atcaaaaagg acctgcagcg gatgatccaa ttcgttgaaa aactgggtga actggatact
     2041 accggcgtcg aacccctgca acacatgagc ccggagatca acgtgctccg ggaagatatc
     2101 ctccagggct ccgtcagccg caaagaagcc ctctcctccg ccccccagac agacggccgg
     2161 tatttcgaag tcccgaaagt tattcgaaaa taatcccacc ccttcctata atgacaccaa
     2221 ctccgctgat acaccttcaa aatatccaca aaagctacta tatgggcaaa caggccctgc
     2281 ccgtactaat gggcgtttcc ctggatattg cgaaaaacga atatgtagcc cttatggggc
     2341 cctccggttc cggcaaaagc accctcatga acatcctggg ctgcctcgat acccccacct
     2401 ccggaacgta tatactcaac ggtcaggacg tcagcaaaat gcccgacgac gacctcgccg
     2461 acgtccggaa tatcgagatc ggcttcgtct tccagcaatt caacctcctc ccccggctca
     2521 ccgccgtgga gaacgtcgcc ctccccctcg tctatgccgg ggtgccgccc cgccagcgca
     2581 tcgaacgggc catggcggta ctggaaaaag taagcctcac cgaccgcagc caccataaac
     2641 caaacgaact ctcgggcggc cagagccagc gcgtcgccat cgcccgcgcc ctggtcaaca
     2701 acccctccct catcctcgcg gacgagccca cgggtaacct cgatacaaaa acctccatcg
     2761 agatcatgga catcttcacc agcatccagg ccgcaggcaa caccgtcgtc ctcgtcaccc
     2821 acgaagagga catcgccaat tacgcccgcc gcatcgtgcg gttgcgcgac ggggtgatag
     2881 aaagcgaccg ccgccgcatc gccgaaacca tcaccacctg accatccccc aatcccacca
     2941 tccgatggca ataaagatct ataccaaaac tggcgacaaa ggcaccacct ccctcatcgg
     3001 cggcacaaag gtctccaaaa gcagcctgcg catcgaagcc tacggaacca tcgacgaact
     3061 caactccttc atcggcctcc taagcgaccg gcaggaccag caaaacctcg ccgggatcca
     3121 accactcctc cgcgagatcc aggatcgact cttcaccatc ggctcctccc tcgcctgcga
     3181 ccccgaaaaa gaaccaaaac taaagatccc cgacctcaaa ccggaagaca taaaactcct
     3241 cgaaacggcc atcgacacca tggagaccga actgcctccc atgaagtcct tcctcctgcc
     3301 cggcggccac cccgccgtct ccaccgcaca catcacgcgc tgcgtctgcc gccgcgccga
     3361 aagatgctgc gtacgcatgc aggacgaagg agtcttcgtc gaacccctcg tgctccaata
     3421 cctcaatcgg ttaagtgatt atttgttcgt gctggcccgc ttcatcggcc accgcctcgc
     3481 ggtccctgag atcccctgga agcccagggt atagcctcat ccatcctctc ccaacctcat
     3541 ccatcccttt cccatcccct catcaatccc gttcccccac acccaccaca ttcccaccca
     3601 acgcatataa ttaactatca acaacttacc atcatcatct ttcgcacata ccccccgccg
     3661 ctcaattggc catcatccca tccttcatat gcaaaacccg gtcactcatc gtcgcgagct
     3721 cctcattatg cgtcacaatg agaaaggtct gttggaaccg atcgcgtaag tcgaagaaca
     3781 gctgatgcag ctccttcgca ttggccgtat ccaggttgcc ggtcggttca tcggcgaaca
     3841 caatgtccgg gttgttgatc agcgcacgcg ccacagccac ccgttgctgc tccccacccg
     3901 acagcgcgcc cggtttgttc tcgacacgat gaccaacccc cagcacattc aacagctcca
     3961 gcgcccgctc ctccacctcc cgcttcttcc ggcccgcgat ccatccgggg atacagacat
     4021 tctccagggc actgaactcc ggcagcaggt gatgaaactg gaaaacaaaa ccgatatgcc
     4081 ggttgcgaaa ggcagccaac ggcttcccct tcaactcatg gatcttctta tcctgcagca
     4141 tcacttcccc cgccgccggc gagtccaatg tgccgagaat atgcagcagg gtactctt
//
LOCUS       DATGFG010000899         7233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_601314, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000899 DATGFG010000000
VERSION     DATGFG010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601314"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VKQ52_17010"
     CDS             <1..1006
                     /locus_tag="VKQ52_17010"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HLZ88960.1"
                     /translation="LTLDGQLQPAGHVLALSSQKELTVDENSGYEKGEIKAGQNFQTE
                     VLHYIRAKKNYLVLVDSSFAAAFPRLARFGRQQFKSDYSIIFVLTNNDPAHYTVKIRQ
                     ETKESPLANVVGILPGKKANEIVIFSGHYDHLGVTKTPVKGDSIYNGANDDASGTTAM
                     IMLANYFAKQKKNERTLVFAAFTAEEIGEYGSQYFSSQFDPATVVAMFNIEMIGSESK
                     WGGNSAFITGYERSDMGKILQKNLAGSSFTFYPDPYPDQQLFYRSDNASLARQGVPAH
                     TISTTQIDKDKYYHQLTDEIGTIDINNMVRIIKAIALSSTSIVDGKDTPTRVDTTQIR
                     "
     gene            1037..2317
                     /locus_tag="VKQ52_17015"
     CDS             1037..2317
                     /locus_tag="VKQ52_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88961.1"
                     /translation="MDNASGLEHCKRLIETSLGWGDSASWTNEDFDSLSDRIFERTSV
                     SLSVSTLKRIWGKVKYDSSPTTATLNALARFAGFEGWRHLLAASQPAPTPATPNTPPL
                     PTSQTPPGRKRSRLTPLIIVTTAIAGLLSFISARIIHKPSGGTTVRFEQHRTSDKLPN
                     SVVFNYDATALHPESVSIQQSWDPTRSEKVDPNGKEHTSIYYYPGFFRAKLIVDGKVK
                     MESDVYVPSHGWKAIIGRDPLPVYLSAAEATVDSGRMGITARTLEAKTGSPVFSGRWV
                     GFFDVRQFPGIRGDHFLFKTTLRNTSTVEQCLCRNVRITLMGTESPIVIPLSDKGCIS
                     GLGLYTGSSGVSGKDHDLSAFGCDLSQWQQLTCTQQNQVLSIWLNGKLIFTLPRNKSI
                     GDLIGLHIAFEGTGEIKAVSLQGDDDEHDLLKER"
     gene            complement(2354..3004)
                     /locus_tag="VKQ52_17020"
     CDS             complement(2354..3004)
                     /locus_tag="VKQ52_17020"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLZ88962.1"
                     /translation="MKIHIGCGSTLLPGFINIDNSPTALLSKMPGFVPSLLGRLSLIN
                     EDQLSFSRMLREKKKEFLYSNCLRLPFKDNTVDFAYSSHMLGWCLGQEQINRFLRELH
                     RVLRPGGGARLSFFDFDKVLADYQQHRNTVRLMGQMPLGAHEFSRGQKLKLLLSPNMQ
                     NGIPLNPETFRDYLEKNAFREVTVLPAGATTMEEEWVKGLDLCQRADESVYMECRK"
     gene            complement(3051..4484)
                     /locus_tag="VKQ52_17025"
     CDS             complement(3051..4484)
                     /locus_tag="VKQ52_17025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLZ88963.1"
                     /translation="MRKYCLMLAIGLPVAGLFAQDSALKTRAMGDIQSRYDDYKKIAL
                     QIWDYAEVGYKEVKSSALLQKTLTDNGFTVKPGVAEIPTAFVASYGSGSPVIGILAEF
                     DALPGLSQQAVPEKMPFEGRNAGHGCGHHLFGTASVAAGIEIKKLIAEKKLSGTVRVY
                     GCPAEEGGSGKVYMVRAGLFNDVDVVIHWHPGAENAVTLTSALANKSAKFRFHGLSAH
                     AAAAPDKGRSALDGVEAMDYMVNMMREHIPQETRIHYVITNGGKAPNVVPDFAEVYYY
                     VRHPKKEEVVRIFDWVAKAAEGAAMGTQTSVDYEVIGGTHDLLLNKALADDMQQNLLR
                     VGGVNYTPAETEFAKKIQASFTYKVAAIETVGTVQPLKERLDGGGGSTDVGDVSYAVP
                     TVGLEAATWVAGTPAHSWQATACGGMEIGTKGMMVAAKTMALTAIDCFLNPALVTSAK
                     EEWRKDKGDYQYKALLGDRKPALNYRD"
     gene            complement(4493..5803)
                     /gene="argH"
                     /locus_tag="VKQ52_17030"
     CDS             complement(4493..5803)
                     /gene="argH"
                     /locus_tag="VKQ52_17030"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HLZ88964.1"
                     /translation="MSGNKLWSKANTSTSQLIETFTVGRDKDFDVLLAEYDVLGSLAH
                     TEMLASVGLLSREDLVLVQDGLKAILGEIRAGSFVIDKDVEDVHSQVELLLTNRIGEA
                     GKKIHSGRSRNDQVAVDIKLYLRAQVLAIKEEVKELFDLLIALSEQYRDKLLPGYTHL
                     QIAMPSSFGLWFGAYAESLVDDLEVLAAAYQVANKNPLGSGAGYGSSFPLDREMTTRL
                     LHFRTLNFNSVYAQMSRGKTEKIVAMGLAAVAATLSKLAMDCCLYINQNFGFISFPPE
                     LTTGSSIMPHKKNPDVFELIRAKCNRIQSTPNELTLLINNLPSGYHRDMQLTKEILFP
                     AIEEGKACLQMTRLMLAHIEIKDDILKDEKYKYLFSVEAVNELVNQGVSFREAYRQVG
                     NQIDEGKFTFDHTRGLHHTHTGSIGNPGNEGIVGEMRSVLMKFE"
     gene            complement(5806..6882)
                     /locus_tag="VKQ52_17035"
     CDS             complement(5806..6882)
                     /locus_tag="VKQ52_17035"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HLZ88965.1"
                     /translation="MSNASIHSLGVEAIELLKRLIAVPSFSKEEDRTAELIVDYLGSK
                     GVAASRLENNVWAKNKYFDERKPTLLLNSHHDTVKPNKAYTLDPFTPIEKDGKLFGLG
                     SNDAGGCLVSLIATFLYFYEEKGLSHNLILAATAEEEISGHHGIELLLPMLPEIQWGI
                     VGEPTKLQLAVAEKGLLVLDCVSHGKAGHAAREEGENAIYKALRDIEWFRDFRAPKVS
                     DLLGPVKMTVTVIDTDNKAHNVVPSQCHFVVDVRVNECYTFEELLEIIRGAVACDVTP
                     RSLRMRSSGISLDHPIVRSGLRLGRTCYGSPTTSDKALMPFPALKTGPGDSARSHTAD
                     EYIYIGEIGQGIEGYIDLLKPVIL"
     gene            complement(6875..>7233)
                     /locus_tag="VKQ52_17040"
     CDS             complement(6875..>7233)
                     /locus_tag="VKQ52_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88966.1"
                     /translation="FAPITHDGKGQLLNTNADTIAQELARALSTGFEVSLVYSFEKSG
                     VLLNAEDDASVIGRINPREYEELKARKVIFAGMIPKLDNAFAALRSGVGRVIIGRAEE
                     LPLLLAGEAGTTILHE"
BASE COUNT         1668 a         2456 c         1666 g         1443 t
ORIGIN      
        1 cctcaccctc gacggccagc tgcagcccgc cggtcacgtc ctcgccctca gcagccagaa
       61 agaactcacg gtcgacgaga acagcgggta cgaaaaaggg gagataaaag ccggccaaaa
      121 ctttcagacc gaggtcctgc actatatccg cgcgaaaaag aattacctcg tgctggtcga
      181 cagctccttc gccgccgcct tcccccgcct ggcccgcttc ggccgccagc aattcaaatc
      241 ggactatagc atcatcttcg tcctcaccaa taacgacccc gcccattata cagtaaagat
      301 ccggcaggag acaaaagaat ccccgctggc caacgtcgtc ggcatcctcc ccgggaaaaa
      361 agcgaacgag atcgtcatct tctccggtca ctatgaccac ctcggcgtga caaaaacccc
      421 ggtgaaagga gattccattt acaacggcgc caacgacgac gccagcggca ccaccgccat
      481 gatcatgctg gccaactatt tcgcaaaaca aaagaagaac gaacggaccc tcgtctttgc
      541 cgccttcacc gccgaagaga tcggggaata tggctcccag tatttctcca gccagttcga
      601 ccccgccacc gtggtggcca tgttcaacat cgaaatgatc ggctccgaaa gcaaatgggg
      661 cggtaactcc gcctttatca ccggctacga acgctcggat atgggcaaga tcctgcaaaa
      721 gaatctggcc ggcagcagct tcaccttcta ccccgacccc tacccggatc aacaactctt
      781 ttatcgctcg gacaacgcca gcctggcaag acagggcgtg ccggcacaca cgatctccac
      841 tacccagata gacaaggata aatactacca ccagctgacc gatgagatcg gcacgatcga
      901 tataaacaat atggtccgca tcatcaaagc catcgccctg agctccacct ccatcgtgga
      961 tggcaaggat acgccaaccc gggtggatac gacacagata cgataagcgt taacccgtct
     1021 tatacttgga caactaatgg acaacgcatc cggtcttgaa cattgcaaac gactcatcga
     1081 gacctcactc ggctggggtg actccgcctc ctggacaaac gaggacttcg acagccttag
     1141 tgaccgtatc tttgaaagaa catcggtaag cctcagcgtc tcgaccctga agcgtatctg
     1201 gggaaaagta aaatatgatt cctccccgac aacggcgacc ctgaacgccc tggcccgctt
     1261 cgccggcttc gaaggatggc gtcacctctt agccgcctcc caaccagccc ccacaccggc
     1321 aaccccgaac accccgccgc tccccacctc gcaaactccc ccgggccgga agcgcagccg
     1381 cctgacccca ctcatcatcg tcaccaccgc catcgccggc ctgctttcct ttatcagcgc
     1441 ccgcatcatc cacaaaccct ccggcgggac cacggttcgc ttcgaacaac accgcacctc
     1501 ggacaaactg ccaaactccg tcgtcttcaa ctacgacgcc accgcactgc acccggagtc
     1561 ggtatcgatc caacagtcat gggacccgac aagaagtgaa aaagtggacc ctaacgggaa
     1621 agaacatacc tccatatatt actatcccgg ctttttccgt gccaaattga tcgtcgacgg
     1681 caaggtaaaa atggaatcgg acgtctatgt cccctcccac ggctggaaag ccatcatcgg
     1741 ccgggacccg ctccccgtct atctctcggc ggccgaagcg acagtcgact cagggcgcat
     1801 gggcatcacc gctaggaccc tggaagcaaa gaccggctcc cccgtcttca gcggccgctg
     1861 ggtcggcttt ttcgacgtcc gccaattccc cggcatcagg ggcgaccatt tcctgttcaa
     1921 aacaaccctc cgcaacacct ccaccgtcga gcaatgcctc tgccgtaacg tccgcatcac
     1981 cctgatgggt accgaaagcc ccatcgtcat cccgctctcg gataaaggct gcatctccgg
     2041 gctgggtctc tacaccggct cctccggcgt cagcggaaaa gatcatgacc tcagcgcctt
     2101 cggctgcgat ctcagccaat ggcaacagct tacctgcacc cagcaaaacc aggtgctgag
     2161 catctggctc aacggcaaac tcatcttcac gctgccacga aacaagtcga taggggatct
     2221 catcggcctc cacatcgcct tcgaaggcac gggagaaata aaagccgtca gcctgcaagg
     2281 cgacgatgac gaacacgatc tgctaaagga aagataaacc tccgccccgc acaccccggc
     2341 caacggaata agcctatttt cggcactcca tatacaccga ctcatccgcc cgctggcaaa
     2401 ggtcaaggcc cttcacccat tcctcctcca tcgtcgtggc gcccgccggc aatacggtca
     2461 cttcccggaa agcattcttc tccaggtaat cgcgaaacgt ctcgggattc aacgggatcc
     2521 cgttctgcat attgggacta agcagcagtt tcagcttctg cccgcggctg aactcatggg
     2581 cgccaagcgg catctgcccc atcagccgca ccgtattccg gtgctgttga taatccgcca
     2641 acaccttgtc gaagtcaaaa aaggaaagcc tcgccccgcc gcccggccgc aacacccggt
     2701 gcagctcgcg aaggaagcgg ttgatctgct cctggccaag acaccagccc agcatatgcg
     2761 aactatacgc aaaatccacg gtattatctt taaagggaag ccggagacaa ttggaataaa
     2821 ggaactcctt tttcttttcc cgcaacatcc gcgagaagct caactggtct tcattgatca
     2881 gcgaaagcct gcccagcagc gacggcacaa agcccggcat cttcgaaagc agggcggtag
     2941 gactattgtc gatattgata aagcccggta ataaggtcga tccgcaacca atatgtattt
     3001 tcatagtgaa accccatttt tggcgccccc gccacgcagg cagctatagc tcaatcccta
     3061 taattcaacg ccggcttccg gtcacccagc aacgccttat actggtaatc ccccttgtcc
     3121 ttccgccatt cctccttcgc cgaggtcacc agggccgggt tcaaaaagca gtcgatcgcc
     3181 gtcagcgcca tcgtcttggc cgcaaccatc atccccttgg tgccgatctc cattcccccg
     3241 caggccgtcg cctgccagct gtgcgccggc gttcccgcca cccaggtcgc cgcctccagc
     3301 ccgaccgtgg gcacggcata actgacatcc ccgacatcag tggaaccccc gccgccatcc
     3361 agccgctcct tcaacggctg caccgtcccg accgtttcga tggccgccac cttataggtg
     3421 aacgaagcct ggatcttttt ggcaaactcc gtttccgccg gtgtataatt caccccgccc
     3481 acccgaagca ggttctgctg catatcatcc gccagcgcct tgttcagcaa aagatcatgc
     3541 gtccccccga tcacctcgta gtcgacactg gtctgcgtcc ccatcgccgc tccttccgcg
     3601 gcctttgcca cccagtcgaa gatccgcaca acctcctcct tcttcgggtg acgcacatag
     3661 taatagacct ccgcaaaatc agggacgaca ttgggcgcct ttccgccatt ggtgatgaca
     3721 tagtggatcc gcgtctcctg cgggatatgc tcccgcatca tattgaccat ataatccatg
     3781 gcctcgaccc catccaacgc cgaacggccc ttgtcgggtg ccgccgccgc atgcgcggac
     3841 agtccatgaa accggaattt ggcggactta ttggcaagcg cactcgtaag cgtaacggca
     3901 ttctccgcac ccggatgcca gtggatcacg acatccacat cgttgaaaag cccggcgcgg
     3961 accatataga ccttacccga ccccccctct tctgccggac acccatacac gcgcacggta
     4021 ccgctaagct tcttctccgc gatcagcttc tttatctcga tacccgccgc caccgaagcc
     4081 gtgccaaaaa gatgatgccc gcacccatga cccgcattcc ggccctcgaa cggcatcttc
     4141 tcgggaacag cctgctggct cagcccgggt aacgcatcga attccgccag gataccgatc
     4201 accggactgc cggaaccata actggccaca aaagccgtcg ggatctccgc tacccccggc
     4261 tttacggtaa acccattgtc agtaagcgtt ttttgtaaca gggcggagct cttcacctcc
     4321 ttatacccca cttcggcata atcccagatc tgtaaggcga tcttcttata atcatcgtaa
     4381 cgggattgga tatcgcccat ggcccgcgtc ttcaacgcgc tatcctgggc aaaaagaccg
     4441 gctacgggaa gaccgatagc cagcatcagg cagtattttc tcatactaca atttactcaa
     4501 atttcatcaa caccgagcgc atctcgccaa caatcccctc attccccggg ttcccgatac
     4561 tcccggtatg cgtatggtgc aatcccctcg tatgatcaaa tgtgaatttt ccctcatcta
     4621 tctggttccc cacctgccgg taggcctccc ggaaagagac cccctggttg acaagctcat
     4681 tcaccgcctc gacactaaaa agatatttgt atttctcatc cttcaggata tcgtccttta
     4741 tctcgatatg cgccaacatc agccgcgtca tttgcagaca ggccttccct tcttcgatgg
     4801 ccgggaaaag tatctccttg gtaagctgca tatcccggtg ataacccgac ggcagattat
     4861 tgatcagcag ggtcagctca ttcggcgtgg actggatccg gttgcacttg gccctgatca
     4921 gctcgaaaac atcggggttc ttcttgtgcg gcatgatact gctgcccgtg gtcagctccg
     4981 gcgggaaaga gataaagcca aaattctggt tgatataaag acagcaatcc atggctaact
     5041 tactcagcgt agccgccacc gccgccagcc ccatcgccac gatcttttcc gtcttccccc
     5101 ggctcatctg cgcatagacg gagttgaaat tgagggtccg gaaatgcaac agccgcgtcg
     5161 tcatctcccg gtccagtggg aacgaagacc catagcccgc cccgctgccc aaagggttct
     5221 tattggccac ctggtaggcc gcggccaaca cttccaggtc atctacaagg ctctcggcat
     5281 aggcgccaaa ccaaagccca aaggaagaag gcatcgctat ctgcaaatgc gtatagcccg
     5341 gtaacagttt atcccggtat tgctcactca acgcgatcag caggtcgaac aattccttca
     5401 cctcttcctt tatcgccagc acctgcgccc gcagatacag ctttatatcg acggccacct
     5461 ggtcattccg gctccggccg ctatggatct tcttacccgc ctccccgatg cggttcgtca
     5521 gcaacagctc cacctgcgaa tgcacatcct cgacatcctt gtcgatcaca aaagaccccg
     5581 ccctgatctc acccaggatg gccttcaacc cgtcctgcac aagcaccagg tcctcccggg
     5641 aaagcaaccc taccgaagcg agcatctccg tatgggccag cgaacccaat acatcgtatt
     5701 ccgccagcag gacatcgaaa tccttgtccc gcccaacggt aaaggtctcg atcaactgcg
     5761 aagtgctggt gttcgcctta ctccaaagtt tgtttccgct catatctata atattaccgg
     5821 tttcaacaga tcgatatagc cttcgatgcc ctgcccgatc tccccgatgt aaatatactc
     5881 atccgccgta tggctccgcg ccgaatcccc cggccctgtc ttcagggcgg gaaaaggcat
     5941 cagcgccttg tccgaagtgg tcggactgcc ataacaggtc ctgcccagtc gcaaccccga
     6001 tcgcacgatc gggtgatcca gcgagatccc cgacgaccgc atccgcaggc tccggggcgt
     6061 aacatcacag gcgactgccc cccggatgat ctccagcagc tcctcgaaag tatagcattc
     6121 attcacccgc acatccacca caaaatgaca ctgggacgga actacgttat gcgccttatt
     6181 atccgtgtcg ataacggtca cggtcatctt caccggcccc agcagatcgg acaccttcgg
     6241 cgcccggaag tcgcggaacc actcgatatc ccgcagggcc ttgtaaatgg cattctcccc
     6301 ctcctcccgc gccgcatggc cggccttgcc atgactgaca caatccagca ccagcagccc
     6361 cttctcggcg accgccagct gcagcttcgt cggctcgccg acgatacccc actggatctc
     6421 cggcagcatc ggtagcagca gctcgatccc atgatgaccc gagatctcct cctcggcggt
     6481 ggccgccagg atcaggttat gggaaagccc cttctcctcg tagaaataaa gaaaagtggc
     6541 gatcagggag acaagacatc cccccgcatc attgctgccg agcccgaaca gcttcccgtc
     6601 cttctcgatg ggcgtaaaag gatccagcgt atacgccttg ttgggcttca cggtgtcatg
     6661 atgggaattg agcaggagcg tgggcttgcg ttcgtcaaaa tatttattct tcgcccagac
     6721 attgttttcc aaccggctgg ccgcaactcc cttcgacccg agatagtcga cgatcagttc
     6781 agccgtccgg tcctcctctt tactaaagga aggcacagcg ataagccgct tcaacaattc
     6841 gatggcttcg actcccaggc tatggatact tgcgttactc atgaagaatg gttgttcctg
     6901 cttccccggc aagcaataaa ggtaactcct ccgcccgccc gatgatcacc cggcccaccc
     6961 cgctgcgcaa cgccgcaaag gcattgtcga gtttggggat catcccggca aagatcacct
     7021 tccgcgcctt tagctcctcg tattcccggg ggttgatccg gccgatgacc gatgcatcgt
     7081 cttccgcatt caacaaaacc ccgctctttt cgaacgaata aacaagactc acctcaaaac
     7141 cggtgctcag tgcccgcgcc agctcctggg caatggtatc ggcattggta ttcagcaact
     7201 gccccttccc gtcatgcgtg ataggcgcaa aga
//
LOCUS       DATGFG010000900         5332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_506827, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000900 DATGFG010000000
VERSION     DATGFG010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506827"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(606..1067)
                     /locus_tag="VKQ52_17045"
     CDS             complement(606..1067)
                     /locus_tag="VKQ52_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88967.1"
                     /translation="MYTGLLSLHSYLRWVILILELVVLYKSFTGMTGGRAFTAGDRKA
                     GLFLMISAHTQLLVGIYLYFAGPWGVATIQNLGFGAVMKDSVARFYAVEHIFGMLVAI
                     VLITIGRGVSKKAIPDAAKHRRSFWLFLVALVIILATIPWPFRAGVARPWL"
     gene            1300..2841
                     /locus_tag="VKQ52_17050"
     CDS             1300..2841
                     /locus_tag="VKQ52_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ88968.1"
                     /translation="MKPTLALITLVSFLTLAAPSIVEAQTTYTVSGGGSKNWSTLSPS
                     PDWCTGCVFNIASNTTLVINATTGCTNCTFNGGSVSVTKNFAFTAGIFNGSAVTISNA
                     SNYYASSPYGGPTFENGSVTFNVGLTCQACNFINETATMSIPAGGTMTMQASGGYNTT
                     TFNNSTLSMTSTSNTTLLYFNSAVSLTNSAITLTNIKMKSDVGKFALSGSQLYLYGTS
                     YSAPTIGPFALTNTSSIFLGDGTASSTAYLDLSSAGANALQIDATSLVKVGADKNYLN
                     DASSYQYTNSTGTSSSVSTANNQLNCNYSGSSGHSNSCSTNLIYGCATVSSGAVACIT
                     LATVDLNLSATAAGPGQVALAFTDAETATADRYQIERNTGNNEWITINTIPAGGYTSG
                     EYRYTDADAPSGTIEYRVARIDQNGKIVYSPISSVTIDQAGSAIGIHPNPATGGTFFI
                     TTPYTGEMVVNVFTMTGQLLYRADLKGQTQYAIHLPTQSATLGSVIVQTLGQGGTHTF
                     TVLVR"
     gene            2871..3911
                     /locus_tag="VKQ52_17055"
     CDS             2871..3911
                     /locus_tag="VKQ52_17055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ88969.1"
                     /translation="MNNSDSGNKRPIVGTLFLVVCCAGLLTMGCNGHSDKKNDQAAVL
                     HHMPYAPLTDSLDQAKGDEAAGLHFRRAELLAHNNLHELAADDYRRSWDLHPDEMTGF
                     RYASTLSIIGQADRAIQVLKECTKKYPANPSFPSMLGELYQQSGKMKDALDIYDELIA
                     KDTGNFEAWYEKGLLLEKARDTAGALLALEKAYSLQPVNTYGLELAHLYAEKRNPAAL
                     SICDGILHKDSTHELLDPLFIKGIYYSNINQYKKAIIQFDSCISRDWKFTDAYLEKGI
                     ALFEQKQYDTAMSTFLMTVRVSNTYPDGYYWVGRCYEATGHKDQAILYYRQAVALDKD
                     FTEAADRIKKLQ"
     gene            3991..4641
                     /gene="rpe"
                     /locus_tag="VKQ52_17060"
     CDS             3991..4641
                     /gene="rpe"
                     /locus_tag="VKQ52_17060"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HLZ88970.1"
                     /translation="MPLIAPSLLASNFLRLEDECKMLNESNADWFHLDVMDGRFVPNI
                     SFGLPVIEQLRKTTTKVFDVHLMIEEPERYAEAFKKAGADRLTVHLEACRHLHRNIQQ
                     IHSLGMKAGVALNPHSPVELLSDVLQDIDTVLIMSVNPGFGGQEFIQHTNQKIRNLRR
                     MIHERGLRTLIEIDGGVTLQNAGIIVKSGADVLVAGSTVFHSDDPKATIHTLKNID"
     gene            4638..>5332
                     /locus_tag="VKQ52_17065"
     CDS             4638..>5332
                     /locus_tag="VKQ52_17065"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ88971.1"
                     /translation="MNVLALILSFSLLALPAFAQKKVPATRKAAVVSGHLLDENENPL
                     AGVTVTILGRQSGLITNDSGFFRIKVPAEKAFALVFSYTGYKSEQRNFLLNENEEEKI
                     VVRLERGAKALEQVVVSDQRDRQEAGLIRVNPKNAINIPTPVGGIESLLKVFVGSNNE
                     LTSQYSVHGGNYDENLTYINDFEVFRPYLVNNAQQEGLSIINPELTRSVNFYTGGFQA
                     RYGDKISSALDIQ"
BASE COUNT         1411 a         1833 c         1096 g          992 t
ORIGIN      
        1 agaaccgaac atccgaatcc tgagagacca ctgatccaaa catcctgaga aagtaccgat
       61 ccagatccac gaaaaccaag cccgtttacg atccgtttat ccaatgagaa ccatcctgta
      121 tccgaaaagc caaatggcca aacctaaatc caaaccgaaa aacagactaa atccacatcc
      181 gaaaaaaaac ctggaagaat gccagggtag aacccgggaa aaaatgtaat ccgaattccc
      241 ttctgaagcc tgaaaaagat cgtagttggc cctgacaaaa aatttgccgc cgcggaaccc
      301 cgcggcggct ttcttttgcc ccatcccccc caaaaaccct tcgtcgggca atcccttccc
      361 tcgtcgccca aatccctccc tccgccgaaa aaacttccaa acccgctccg actttaccaa
      421 ctttgtatca gcaaacgaaa acgatccaaa tccgaagaac aaataatcca aaaaaaccct
      481 gaaaacccaa tcggccaaca tcctcagaaa aaccaatcca tcgatgaaac cctaaaaaaa
      541 attgccgccg cgatagcatc gcggcggctt tccttttagc actacacaac gtatagccgc
      601 acaaactata accacggcct cgcaaccccc gccctgaacg gccaggggat cgtcgccagg
      661 ataatcacca acgccaccag gaacaaccag aaactccgcc ggtgcttcgc cgcgtccggg
      721 atcgctttct tcgacactcc acgcccgatc gtgatcagca cgatcgccac cagcatcccg
      781 aaaatatgct ccaccgcata gaaccgcgcc acgctatcct tcatcaccgc gccaaaccca
      841 aggttctgga tcgtcgctac cccccaggga cccgcgaaat acaggtagat ccccaccaat
      901 aactgcgtat gcgccgatat catcaaaaac aaccccgcct tcctgtcccc cgccgtaaac
      961 gcccttcccc ccgtcatacc ggtaaaactc ttgtaaagaa caaccaattc taaaataagg
     1021 atcacccatc ggagataact atgcagagat aacagtcccg tgtacatgta aatggatttt
     1081 aagctgttag aaaaatgtgc ccaaagctac gaccgccttc ctgaaaagga aaattaatct
     1141 ccccattccc atacccggga aaccccttcc cggcctggga aaaacctaaa gtaaacactt
     1201 aatcacattc attgacaatc aatcacttac atttccggca cccttttaga ccacctagga
     1261 caaattcaag ttccttatcc cgcagaaaaa caattcgata tgaaaccgac cctggccctg
     1321 attaccctgg tatccttcct taccctcgcc gccccttcca tcgtcgaagc acagaccact
     1381 tataccgtat ccggtggcgg ttccaagaac tggtcgacac tgagcccctc ccccgactgg
     1441 tgcacaggct gcgtcttcaa tatcgccagc aacaccaccc ttgtcatcaa tgccaccacc
     1501 ggctgtacca actgtacctt caacggcggc agcgtctccg tcaccaagaa tttcgccttc
     1561 accgccggta tcttcaacgg ctccgccgta acgatcagca acgccagcaa ctactatgca
     1621 agcagccctt acggcggacc tacattcgag aacggaagcg tcaccttcaa cgtggggctc
     1681 acctgccagg cctgtaactt catcaatgag acggcaacca tgagcattcc tgccggcggc
     1741 acaatgacca tgcaagcctc cggtggatat aacactacca ccttcaacaa cagcaccctg
     1801 agcatgactt cgacatccaa cacgaccctg ctctatttca attccgccgt cagccttacc
     1861 aactccgcca ttacactgac caatataaag atgaagagcg acgtcggcaa gttcgccctc
     1921 tccggcagcc agctctatct ttacggaaca tcttattcgg cgccgaccat cggtccgttc
     1981 gccctcacca ataccagttc tatcttcctg ggcgacggca ccgcctccag cacggcctac
     2041 ctcgacctga gcagtgcggg agccaacgcg ctccagatcg acgccacctc attggtaaaa
     2101 gtaggcgccg ataagaatta cctcaacgat gcctcctcct atcaatacac caactccacc
     2161 ggtacctcct cctccgtctc cacagccaac aaccaactca attgtaacta ttcaggctcc
     2221 agcggccatt ccaacagttg ctccaccaac cttatctacg gttgcgccac ggtaagctcc
     2281 ggcgccgttg cctgtatcac cctggccacg gtagacctga acctttccgc gacagcggca
     2341 ggccccggcc aggtagccct tgccttcacc gacgcggaaa cagccacggc cgatcgctac
     2401 cagatcgaac gcaacaccgg caataacgaa tggattacca tcaacaccat ccccgccggc
     2461 ggctatacat cgggtgaata ccgctacacc gacgcggatg caccctccgg cacgatcgaa
     2521 taccgcgtcg ccaggatcga ccagaatggt aagatcgttt actcccccat ctcctccgtc
     2581 accatcgatc aggccggcag cgccatcggc atccacccca acccggcaac gggcggaacc
     2641 ttcttcatca ccacgcccta caccggtgag atggtggtca acgtcttcac gatgacaggc
     2701 cagttgctct accgcgccga cctgaaggga caaacccaat acgccatcca cctgcccaca
     2761 cagtcggcta ccctcggctc cgtcattgta cagaccctcg ggcagggcgg cacgcacacc
     2821 tttaccgtgc ttgtccggta agattcggga ccaatgctta tttttgtccc atgaacaatt
     2881 cggatagcgg aaataaacgg ccgatagtag ggaccctttt cctggtggtt tgttgcgcgg
     2941 gactcctgac gatgggatgc aacggacata gtgataaaaa gaatgaccag gcggccgtcc
     3001 tgcatcatat gccctatgct cccctgaccg acagcctcga ccaggcaaaa ggcgacgaag
     3061 ccgcagggct ccacttccgc cgcgccgaat tactcgccca caacaacctc cacgaactgg
     3121 cggccgatga ctaccggcgc tcatgggatc tgcaccccga tgaaatgaca ggcttccgct
     3181 atgcttccac cctctccatc atcggccagg ccgatcgggc catccaggta ctaaaggaat
     3241 gcacaaaaaa atatccggcc aacccctcct ttccctcgat gctgggcgaa ctctatcagc
     3301 aatccggcaa aatgaaagat gcgctggaca tctacgacga attgatcgcc aaggacaccg
     3361 gcaacttcga agcctggtac gaaaaaggcc tgctgctgga aaaagcccgg gacaccgccg
     3421 gcgccctcct tgccctggaa aaggcctata gcctccaacc cgtcaacacg tacggcctcg
     3481 aactcgccca tctctacgca gaaaagcgca accccgccgc cctgtccatc tgcgatggca
     3541 tcctccacaa ggattccacc cacgagctcc tcgacccgct cttcataaaa ggcatctact
     3601 actccaacat caaccagtat aagaaagcca tcatacaatt cgattcctgt atcagccggg
     3661 actggaaatt cacggacgcc tacctcgaaa aaggcatcgc cctcttcgaa caaaaacaat
     3721 acgacaccgc catgagcacc ttcctcatga cagtgagagt atccaacacc taccccgacg
     3781 gctactattg ggtcggccgc tgctacgagg ccaccggcca taaggaccag gccatcctct
     3841 actaccgcca ggcggtagcc ctcgacaaag acttcaccga agccgccgac cggatcaaaa
     3901 aactacaata accccaaccg cccctatttt ccaatttttt gtcgcgacgc agtttcccta
     3961 tctttaagcc ggacaaaaaa gacagattct atgcctctta tagcgccctc tttgttggcc
     4021 tcgaattttc tccgcctcga agatgaatgc aaaatgctga acgaaagcaa tgccgactgg
     4081 ttccacctcg atgtcatgga cggaagattc gtccccaata tcagcttcgg cctgcccgtc
     4141 atcgaacaac tccgcaagac gacaacaaag gtcttcgatg tccacctgat gatcgaagaa
     4201 cccgaacgct atgccgaggc ctttaaaaaa gccggtgccg acaggctcac cgttcacctg
     4261 gaagcctgcc gccatcttca ccgcaatatc cagcagatcc attcgctggg gatgaaagcc
     4321 ggcgtagcgc taaaccccca ttcccccgtc gagctcctct ccgatgtcct gcaggatatc
     4381 gacacggtcc tgatcatgag cgtcaacccc ggcttcggcg gccaggagtt catccagcac
     4441 accaaccaga agatccgcaa cctccgccgg atgatccacg aaagaggact caggaccctc
     4501 atcgaaatag acggcggcgt caccctccaa aatgccggca tcatcgtaaa atccggcgcc
     4561 gacgtcctgg tggccggaag caccgttttc cattccgacg acccgaaagc aaccatccat
     4621 acacttaaaa atatagattg aacgtcctcg ccctgatcct ctctttctcc ctgctggccc
     4681 taccggcgtt tgcccaaaaa aaggtccccg ccacacgcaa ggctgccgtt gtctccggcc
     4741 acctcctcga cgagaacgag aacccgctcg cgggcgtcac cgtgacgatc ctcggccgcc
     4801 agtccggtct catcaccaac gactcgggtt tcttccgcat aaaagtcccg gccgaaaaag
     4861 ccttcgccct cgtcttctcc tacaccggct ataaatccga acaacgtaat ttcctgctca
     4921 acgagaacga agaagaaaag atcgtcgtcc gcctggaaag aggcgcaaaa gccctcgaac
     4981 aggtggtcgt ctcagatcag cgcgatcgcc aggaggcggg tctcatccgg gtaaacccca
     5041 aaaatgccat caatatcccc acccccgtcg gcgggatcga aagcctgctg aaggtcttcg
     5101 tcggctccaa taacgaactc acctcccaat actccgtcca cggcggcaac tacgacgaga
     5161 acctcactta tataaacgac ttcgaggtct tccgccccta tctcgtcaac aacgcgcagc
     5221 aggaagggct cagcatcatc aaccccgaac tgacccgcag cgtgaacttc tataccggcg
     5281 gcttccaggc ccgctatggc gacaagatct cctccgcact cgatatccaa ta
//
LOCUS       DATGFG010000901         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_870797, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000901 DATGFG010000000
VERSION     DATGFG010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870797"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VKQ52_17070"
     CDS             <1..585
                     /locus_tag="VKQ52_17070"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLZ88972.1"
                     /translation="LHPGQNWIKIYPMQDADHTAPYWEPTTCMHCDEPPCVKVCPVDA
                     TFKRQDGIVLIDSDRCIGCRFCMAACPYSTRVFNWEDPGLPEAVAKQPYSCESSVPQK
                     KGTVGKCDFCADMTRKGMLPHCVSACPNGVFFFGDMVEDSVTNGAETFRFSDLIRDKA
                     GYRLMEDLGTKPSVYYLPPVNRNFPFVNEEEKAG"
     gene            627..1934
                     /gene="nrfD"
                     /locus_tag="VKQ52_17075"
     CDS             627..1934
                     /gene="nrfD"
                     /locus_tag="VKQ52_17075"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HLZ88973.1"
                     /translation="MQPLAEQKIIEDLLPQRFGRKGVLWVVVLSVISLAGLIAYIHQL
                     SKGLVVTAMRDYVSWGIYISNFVFFVAISLVGSLITAVLRLTNVQWSTPLTRIAEIIA
                     VAAITFASLIIIVDMGRPDRFYNLFIHGRLQSPIIWDVIVITTYFFISLLLLYFPLLP
                     DLAIGLRSRERMGRGLTRLYAFLGKFWKNSPAQVKISERAIGILAIVIIPVAFAIHTV
                     TSWLFATTYRPGWDSTNFGAYFISGAFLVGAGAVLVAMYIFRRAYRLERYITEDHFDR
                     MGKIVVMLALLYLYFNVNEFLVPAFKMKEHEEEHLMGLFTGEFAPVFWFAILTAMIIP
                     ILILVTKKGRRPFPAFLAGALVVIGSWFKRYLIVTPTLLHPFLPMRDVPAEYRHYFPS
                     AEEWAIAMGSLAGMMLIITLFARIFPIVPIQETLTNIEEHEKA"
     gene            1921..>2747
                     /locus_tag="VKQ52_17080"
     CDS             1921..>2747
                     /locus_tag="VKQ52_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88974.1"
                     /translation="MKKLSYYAALALVIFQVPALPGFAQPEKKDLLLQLAYYNDNNHL
                     QYLKAVAKTKTEGKFRFVPGIGLRFYITADSPGYFLGSGLTNDKGEAVVFLPATAKSE
                     WIRSAKQSFVVVSGAAMGFDPVRATADLTKARLKIDTAGGRKVSVSLMESRDSGWAPV
                     KGVDVKVAVKRLDGNLTIADAPTYTTDSTGLVQADFKRDSLPGDAKGNLVLVASVEDN
                     DNYGNLTTEFSVPWGVYRPYVSAYDTRSLFARRGRSPAWLEGIAYSIIVLVWGVLLYL
                     "
BASE COUNT          561 a          752 c          808 g          626 t
ORIGIN      
        1 ttgcatcccg ggcagaactg gatcaagatc tatcccatgc aggacgcaga ccatacggcg
       61 ccctattggg agccgacgac gtgcatgcat tgcgacgaac cgccctgtgt caaggtatgc
      121 ccggtggacg ctaccttcaa aagacaggat gggatcgtgc tgatcgacag cgaccggtgc
      181 atcgggtgcc ggttctgcat ggcggcctgt ccctattcca cgcgggtctt taactgggag
      241 gacccgggtc tgcccgaggc cgttgcgaag cagccgtact cttgcgagtc cagcgttccc
      301 cagaaaaagg ggacggtggg aaaatgcgat ttctgcgccg acatgacccg gaaggggatg
      361 ctgccgcatt gtgtgtctgc ctgtcccaat ggcgtcttct ttttcggcga catggtagag
      421 gactccgtca ccaacggcgc cgagaccttc cggttcagcg acctgatccg ggataaggcc
      481 ggctatcgcc tgatggagga cctgggcacc aagccgagcg tatattatct cccgccggtg
      541 aaccggaatt tcccctttgt caatgaagag gagaaagccg ggtaagaaga aggcggagaa
      601 agtcaaacat caaaaaaatc gaaaccgtgc aacctttagc agaacaaaaa ataatcgaag
      661 acctgttgcc ccagcgtttt ggcaggaagg gcgtcctttg ggtcgtcgtc ctttcggtga
      721 tcagccttgc ggggttgatc gcttatatcc accagttgtc gaaagggctg gtggtgacgg
      781 ccatgcggga ttatgtatcc tggggcatct atatttccaa ttttgtcttt tttgtagcga
      841 taagcctggt gggcagcctg atcacggccg tgttgcggct gacgaatgtg cagtggagca
      901 cgcctttgac gaggattgcg gagatcatcg ccgtggctgc catcaccttt gcctctctta
      961 tcatcatcgt cgacatgggg aggccggacc ggttctacaa cctgttcatc cacgggcggc
     1021 tgcagtcgcc catcatctgg gacgtaatcg tgatcacgac ctattttttt atcagtctcc
     1081 tgttgctgta ttttccgctg ctgcccgatc ttgcgatcgg gttgcggtcg cgggaaagaa
     1141 tgggccgcgg gctgacccgg ctatatgcct tcctgggcaa gttctggaag aacagcccgg
     1201 cccaggtgaa gatcagcgag cgggcgatcg ggatcctcgc catcgtcatt attcccgtcg
     1261 cctttgccat tcataccgtg acctcctggt tattcgcgac tacttaccgg ccgggctggg
     1321 acagcacgaa tttcggggcc tatttcattt cgggagcctt ccttgtcgga gccggggcgg
     1381 tgcttgtcgc catgtatatc ttccggcggg cataccggct ggagcgttat atcaccgaag
     1441 accatttcga ccgaatggga aagatcgtgg tcatgcttgc cctgttgtat ctctatttca
     1501 atgtcaacga attcctggta cccgccttta agatgaagga acacgaggaa gagcacctga
     1561 tgggcctgtt cacgggcgag tttgcgccgg tgttctggtt tgccatcctg accgcgatga
     1621 tcattcctat cctgatcctg gtaacaaaga agggccggag gccgtttccc gccttccttg
     1681 cgggggccct ggtagtgatc ggctcctggt tcaaacgata cctgatagtg acccctaccc
     1741 tgctgcatcc tttcctgccg atgcgggatg ttcccgcgga gtaccgccat tattttccca
     1801 gcgcggagga atgggccatc gccatggggt cgctggcggg aatgatgttg atcattaccc
     1861 tgtttgccag gatcttcccg atcgtcccca tccaggaaac cttaacaaac atagaagaac
     1921 atgaaaaagc ttagttatta tgcggcattg gcgctggtaa tcttccaggt gccggcgctg
     1981 ccgggatttg cgcaaccaga aaagaaggac ctgcttttgc aactcgccta ctacaacgac
     2041 aataaccatc tccaatacct gaaggccgtc gccaaaacga agacggaggg caaattccgg
     2101 ttcgtgccgg gtatcggcct gcgattctat atcacagccg acagccccgg ctatttcctg
     2161 ggcagcggcc ttacgaatga caagggggaa gcggtggtct tccttcctgc caccgccaag
     2221 agcgaatgga tcaggtctgc gaaacagagc tttgtcgtgg tttccggcgc cgcgatgggt
     2281 tttgacccgg tgcgcgccac tgccgacctt accaaggcga ggctgaagat agacacggcc
     2341 gggggcagga aggtcagcgt cagcctgatg gaatcaaggg atagcggctg ggcgccggtg
     2401 aaaggcgtcg acgtgaaggt tgcggtaaaa aggctggacg gaaatcttac catcgccgac
     2461 gcgcccacct ataccacgga ttcgacgggt ctcgtgcagg ccgacttcaa acgcgattcc
     2521 ctgcccgggg atgcgaaggg caacctggta ctggtagcca gtgtggagga caacgacaat
     2581 tacggcaacc tgacgaccga gttttctgtg ccctggggcg tttaccggcc ttatgtttcg
     2641 gcatacgata cccgctcctt gtttgcccgc agggggagat cgcccgcctg gctggaaggc
     2701 atcgcctatt ccattatcgt cctggtgtgg ggcgtgttgt tatatct
//
LOCUS       DATGFG010000902         7474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_669692, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000902 DATGFG010000000
VERSION     DATGFG010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669692"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2578
                     /locus_tag="VKQ52_17085"
     CDS             <1..2578
                     /locus_tag="VKQ52_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88975.1"
                     /translation="TTKKGTRGKPIINASTSVSQERVSYMPAYQERFGSNGGEKWVND
                     FNNLSDAIPYENQAYGPEFRPGAMVPIGRPVYDGTYQLIPYQAVKNQKRDFFDNALTT
                     QNNFSYSAGDDNSRFFVSAQDVSTKGIMPGDKGRRDVFRVGGSKTSGIFSTDFTLSYT
                     YKNTDLTSQPNVVYDNLIQTPADIPITKYKNWATDKFASLDGYYNDYYTNPYWTLAND
                     RSVTRDNSLEGNVHLQLKPTSFLTFSYRLSLNNLNRNNDVSNNGQTYSKYASTGSSLV
                     LYSNYAGNGVDSAIEGNKYNASLGNTFPTYQTATFSNFLVTQDFLATFNKNLSRDVTL
                     TATVGTTYLDNQIKGQIINAGNLFFPVYNVSSLTGIPNVQSVREEARKLGYFGEATVG
                     YKSYAFLHGSYRTDIDSRLSKDNRYIPYYDVDASLVLSDMIPSIGQSQGLNYIKLRAA
                     HSLTGNVSAIAQGSPYVADGAYATVPTLITANGAPTNLGFPFNGVGGYALNNTIANPN
                     IKPEKVTENEVGLELGYNNIVSLTLGAYEQKLTDGIVYAQIPNSSGYTKALVNAASTR
                     NRGLEMELQTTIIHTRDWTWKMGANYTHIDSKVLTINGSQQSLTISQTVNPFLNAGQN
                     NGANANSFAVVGHPYPVIESYDWNKDAAGHVIVDAVTGMPTKSSNLSILGNSNPTDII
                     GITTNLSWKRFSFSATADYRGGYKIFNVIGNAMDFSGVGATTAATGRQRFVFPNSVIV
                     VNGKSVTNTNVTVSDANFNFWPSLYNSVGANYVVSADAWKLREVTVSYDIPKTIFAAT
                     RIIQKAQVIVSGRNLIMIRPSTNKWTDPEFSEGTGNDTGRTGEGQAPPTRIFTATLAL
                     TF"
     gene            2609..4129
                     /locus_tag="VKQ52_17090"
     CDS             2609..4129
                     /locus_tag="VKQ52_17090"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ88976.1"
                     /translation="MLKRIRTYITVSASLLLLATAGCKKGTFDINSPNPNTPSSVSPQ
                     FLLSSALTASAQSSFAAGFVDFANLWSGYYAFSGDYGGYGTTATYNINNGYANANWDY
                     VYAEILVNYQYIIDNSRTAQEANYFGIGQIMQAFHYQRLVDIYNNIPYTDALKGGVLN
                     YPKYTDAATVYQGIIKQVDSGIAAIKGATANADNPGKYDVMFGGNMSNWILFGNTVKL
                     KILLNLTQWSGGPALIKSELNGLTTSSFLGAGQDAAINPGYSNSANNQLNPTWQNTGF
                     TVGGSDQGDHDFYRANSYAVNWYLNNGDPRAARFYAPNANGVIRGRAYGSQDGTEHNL
                     LISSMGPGILQSPSQSAYILPAFESLFFQAEALQRGYISSSSSLQTVYQTAVQESFRL
                     LYSNVTGASYKDTAAAYYGQPNPKTNFTLTSNPLTFIMTQEWAALNQYDPVTSWNNWK
                     RLGIPSDLPVSIYPGTTAAHAPIRLIYPVSEYTANAANVSAQGNIDVINGKIFWMP"
     gene            4150..5070
                     /locus_tag="VKQ52_17095"
     CDS             4150..5070
                     /locus_tag="VKQ52_17095"
                     /inference="COORDINATES: protein motif:HMM:NF020111.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HLZ88977.1"
                     /translation="MKRISIVIPFLIAVTLSACLKDKPNTDFSSTQSTYVSEISTAST
                     SGTTDAPASGLAYFNAATLSLSTASDPDSVFFTVNIASDYPPTKDVPVTLKVDQQALT
                     NYNAGGPATVFEMFPDSTFSFPTKTGTIKAGHRLDTFWVIFHPSMVDPTHSYMLPISI
                     SSASGTTISGNMGTIYFHAVGNPLAGQYNQEWIRYNNATGTGTPAYDLQVGPSSFIPI
                     TPTEITVTSGTGMAYILDFTNTGGVLSNFSVSFPSSGNGSATANGITITGGPTIVLAD
                     GVNKKFTFNFTYNNSAGSARNITDIFTPYP"
     gene            5158..5883
                     /locus_tag="VKQ52_17100"
     CDS             5158..5883
                     /locus_tag="VKQ52_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88978.1"
                     /translation="MRKLIPLLVLPTALSLAAGAQSNPNSITINNAIQDVAAGSTTGF
                     VNFHAPKENTKGRRMLLADFSPGYVTTTNDSALKDPRLVFNYDKITHDLYYSFDRKTV
                     IEADRSRVKTFHLATADGEQEYTRLDLIKPDAFFQTFNSFDNTHYGFYALTKTEFKKA
                     DYRTDGMVESGNNYDEYVDNIQYYIVSPGGKEYQPLELKKKSVRAALPAVKTKVEEYL
                     SQHKNDDVNETFVKNLIEYINKG"
     gene            6115..>7474
                     /locus_tag="VKQ52_17105"
     CDS             6115..>7474
                     /locus_tag="VKQ52_17105"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ88979.1"
                     /translation="MRKLLTLFAVAMLFSVLAIAQTKTLSGKITDAKGQPVPFATVRI
                     KGTKAGVSADADGNYSIKVSPSQTLIVTGAGMAPTEVVAPDAPTFNITVAMKDASMTE
                     VVVTALGIRRSKNELPYSVQRIGGDEVSKERSGNFITNMEGKVSGVTINQANTLGGST
                     NVILRGYKSALGDNQALFVIDGVPIDNTNTTSINQVPGTQGTQSSGLGGYDYGNAAAD
                     INPDDIASISVLKGAAASALYGERGFNGVIIITTKKGRKGLGITVNGGVTTGAIDKST
                     FAKYQHTYGANYGSANGYGSPDGNFLYFDVLGNGTNDLVTPTTEDASWGAKFDPNLMV
                     YQWEAFDKTSSTYHKATPWVAAQHDPTTFWTKPFSYNGSVFIEGGNDKSTFTAGYTRN
                     SDRGILPNSQILKDMVNTSGTLNITDKLTAGASINFTNISGLGRYGTGYASNNEATNF
                     REW"
BASE COUNT         1881 a         2296 c         1704 g         1593 t
ORIGIN      
        1 caccaccaag aaaggcaccc gcggcaagcc gatcatcaat gcctctacca gcgtttcgca
       61 ggagcgcgtt tcctatatgc cggcctacca ggagcgtttc ggttccaacg gcggtgagaa
      121 atgggtgaac gacttcaaca acctcagcga cgctatcccc tacgagaacc aggcctatgg
      181 ccctgagttc cggcccggcg ccatggtgcc gatcgggcgt ccggtctatg acggaaccta
      241 ccagcttatt ccttatcagg ccgtcaagaa ccagaaaagg gatttcttcg ataacgccct
      301 gactacccaa aacaacttct cctatagcgc cggcgacgac aacagccgtt tcttcgtcag
      361 tgcccaggac gttagtacaa agggtattat gcccggcgac aaaggaagaa gggatgtctt
      421 ccgtgtaggc gggtccaaga cctccggtat cttctccacc gatttcacgt tgtcttatac
      481 ctataagaac accgacctta cttcccagcc caatgtcgtg tatgataatc tcatccagac
      541 accggccgac attcccatta ccaaatataa gaactgggct acggacaagt tcgcgagcct
      601 ggacggctac tacaacgact actacaccaa tccctactgg acattggcca atgaccgtag
      661 cgtcacccgc gacaactccc tggaaggtaa cgttcatctc cagttgaaac ccacttcctt
      721 ccttacgttc tcctaccggc tttcgctcaa caacctgaac aggaacaacg atgtgagcaa
      781 caacggccag acctattcca aatatgcgtc caccgggagc agccttgtcc tttactccaa
      841 ctatgcgggt aacggcgtcg actctgccat cgagggcaat aaatacaatg ccagccttgg
      901 caacaccttc cctacctatc agaccgcgac tttctccaac ttcctggtca cccaggattt
      961 cctggctacc ttcaataaga acctgagccg ggacgtcacc ctgacggcca ccgtcggtac
     1021 cacctacctg gacaaccaga tcaaggggca gatcatcaat gccggtaacc tcttcttccc
     1081 tgtttacaac gtgagcagcc tcaccggtat tcccaatgtg caaagcgttc gggaagaggc
     1141 ccggaaactg ggttacttcg gggaagcaac ggtcggatac aagagctatg cctttctcca
     1201 cggttcttac cggacggaca tcgattcccg cctgtccaag gacaaccgct atattcccta
     1261 ttatgatgtg gatgcatcgt tggtcctcag cgacatgatt ccctccatcg ggcagagcca
     1321 gggcctcaat tacatcaagc tcagggccgc tcactcattg acgggtaacg tatccgctat
     1381 cgcccagggc tcgccctatg tggccgacgg cgcttacgca acagtgccta ccctgatcac
     1441 tgccaacggc gctcccacca acctcggttt tcccttcaac ggagtaggcg gctatgcact
     1501 gaacaatacc atcgccaacc ccaatatcaa gccggaaaaa gtaaccgaaa atgaagttgg
     1561 cctcgaactc gggtataata acatcgtgtc gttgaccctg ggtgcttacg aacaaaaact
     1621 cacggatggt atcgtatacg cccagatccc caactccagc ggctatacca aggccctggt
     1681 gaatgcggca agcacccgga accggggact ggaaatggaa ttacagacca cgatcatcca
     1741 tacccgcgac tggacctgga aaatgggcgc caactatacg cacatcgata gcaaggttct
     1801 taccatcaac ggcagccagc agagcctgac catctcccag acggtgaacc ccttcctcaa
     1861 tgccggccag aacaatggcg ccaatgccaa ctcgttcgcc gttgtcggtc acccgtatcc
     1921 tgtcatcgaa tcctatgact ggaacaagga cgcggccgga catgtgatcg tcgacgccgt
     1981 caccggtatg ccgaccaaga gctcgaacct gtcgatcctg ggtaattcca acccgaccga
     2041 tatcatcggt atcaccacca atctcagctg gaagcggttc agcttttctg caaccgcaga
     2101 ctatcgcggc ggatacaaga tcttcaacgt tatcggtaat gccatggact tctccggcgt
     2161 aggcgcgaca acggctgcta ccggccgtca gcgcttcgtg ttcccgaact ccgtgatcgt
     2221 cgtcaatggc aaatcggtta ccaataccaa tgtgaccgta tcggacgcca acttcaattt
     2281 ctggcccagc ctgtacaact ccgttggcgc caactatgtg gtcagcgccg atgcctggaa
     2341 actgcgggaa gtgaccgtgt cttacgatat tcccaagacc atttttgcag cgacccggat
     2401 catccaaaag gcccaggtca tcgtttccgg cagaaacctg atcatgatcc gtccttctac
     2461 caacaaatgg acagatcctg aattcagcga aggcaccggt aatgatacgg gacgtacggg
     2521 agaaggacaa gctccgccta cccggatctt cactgccacc cttgctctca ccttctaaca
     2581 ttgttaattg aagtcaaaaa gaagtactat gcttaaacga attagaacat atatcacggt
     2641 ctcggcgtcc ctcctcctgc tggctacggc aggctgcaaa aagggcacct tcgatatcaa
     2701 cagtcccaac cccaacaccc cctcgagcgt ttcgccccag ttccttttgt cctcggcgct
     2761 gacggcctcc gcccagtctt cctttgcggc aggcttcgtg gatttcgcca acctctggag
     2821 cggctactat gccttcagcg gcgactatgg tggatatgga acaaccgcca cctacaatat
     2881 caacaacggc tatgccaacg ccaactggga ttatgtgtat gcggagatcc tggtgaacta
     2941 ccagtatatc atcgacaact cccgcacggc ccaggaggcg aactatttcg ggatcggcca
     3001 gatcatgcag gccttccact accagcgcct ggtggacatc tataataata tcccctatac
     3061 cgatgcgctg aaaggcgggg tactcaacta cccgaaatac accgacgccg ccaccgttta
     3121 ccaggggatc atcaaacaag tggattcggg tatcgccgcc atcaagggcg ccaccgccaa
     3181 cgccgataac ccggggaaat atgatgtcat gttcggcgga aatatgagca actggatcct
     3241 cttcggcaat acggtcaaat tgaagatcct gctcaacctt acccaatggt ccggtggccc
     3301 cgcattgatc aagagcgagt tgaatggtct gaccaccagc agcttccttg gtgctggcca
     3361 ggatgccgcg atcaatcccg gttactccaa cagcgccaac aaccagctga accccacctg
     3421 gcagaacacc gggttcacgg ttggtggctc cgaccagggc gaccacgatt tctatcgtgc
     3481 caactcctac gccgtgaact ggtatctgaa caacggcgac ccccgggcag cgcgattcta
     3541 cgcgcccaat gccaacggtg ttatccgtgg ccgcgcctac ggcagccagg acggtaccga
     3601 acataacctc ctgatctctt cgatgggtcc cggcatcctg cagtcgcctt cgcaaagcgc
     3661 gtatatcctg ccggccttcg aaagcctgtt cttccaggcc gaagccctgc aacgtgggta
     3721 tatctccagc agttccagcc tccagaccgt ttatcaaacg gcagttcagg aatctttccg
     3781 cctgctttat tctaatgtca ccggcgcaag ttataaggac acggctgctg cctactacgg
     3841 ccaacccaat cccaaaacca acttcacgct gacctccaac ccgcttacct tcatcatgac
     3901 ccaggaatgg gccgcgctca accagtacga cccggtcacc tcctggaaca actggaagcg
     3961 tctcggcatt ccttctgacc tgccggtatc catctaccct ggtacgacgg ctgcgcatgc
     4021 ccccatcaga ctgatctatc ccgtcagtga gtacaccgcc aacgcggcga atgtcagcgc
     4081 acagggtaac atcgatgtca tcaacggtaa gatattctgg atgccttaat catctttaac
     4141 acatttatca tgaaacgaat ctcaatagta attccctttc ttatagcagt cacgctctcg
     4201 gcatgtttga aggacaagcc gaatacggac ttctccagta cgcaaagcac ctatgtctcg
     4261 gagatcagta ctgcgagcac cagcggtacc accgacgccc cggccagcgg actggcctat
     4321 ttcaacgccg cgaccctttc gttaagtacg gcctcggatc ccgattccgt gttctttacg
     4381 gtgaacatcg cctccgatta tcctccaacc aaggatgtcc ctgtgactct gaaagtagat
     4441 cagcaggcgc tcaccaacta taatgccggc ggtcctgcca cggtgttcga gatgtttccc
     4501 gattccacct ttagctttcc cacaaagacc ggaacgataa aggcgggtca ccggctcgat
     4561 accttctggg tcatcttcca tccgagcatg gtcgacccca cccacagcta catgctgccc
     4621 atctcgatct cttccgcctc gggtacaacc atcagcggca atatgggaac gatctatttc
     4681 catgccgtag gaaaccccct ggcgggccaa tacaaccagg agtggatccg gtataataac
     4741 gcaaccggca ccggcacacc tgcttatgac ctgcaggtcg gaccctcgtc ctttattcct
     4801 attacgccta cggagatcac tgttacaagc ggcaccggga tggcctatat cctggacttt
     4861 acgaatacgg gcggggtcct gagcaatttc agcgtgtcct tcccatcctc cggaaacggc
     4921 tcggcaacag ccaacgggat cacgatcacc ggtggtccga cgatcgttct cgccgacggg
     4981 gtgaacaaga aattcacctt caatttcacg tataataact ctgctggctc agcgcgtaat
     5041 attacagaca tattcacgcc ctacccataa ttaaaatgat atgaaaatcc aggccggctc
     5101 ccccaggagc cggccttttt tttgaaaatt ccttatcttg agacttaaaa ttagaccatg
     5161 agaaaactta tcccgctttt agttctgccc actgccttat cccttgcagc cggcgcccaa
     5221 tcgaacccga attcgatcac catcaacaat gcgatccagg acgttgccgc cggatccacc
     5281 accggttttg tgaatttcca cgcacccaag gaaaatacca agggccgccg catgctcctc
     5341 gccgatttct ccccgggcta tgttaccaca acaaatgact cggcattaaa agacccgaga
     5401 ttggttttta attacgataa gataacccac gacctctatt actccttcga ccggaagacc
     5461 gtcatcgaag ccgaccggtc gcgggtcaaa accttccacc ttgcaacggc cgatggagag
     5521 caggagtata cccggctcga tctcataaag ccggacgcct tcttccagac cttcaattcc
     5581 tttgataaca cccactacgg attttatgcg ctcactaaaa ccgagttcaa aaaagccgac
     5641 tatcgcacgg acggcatggt cgaatctgga aataattatg atgaatatgt cgataatatt
     5701 caatactata tcgtttcacc cggcggaaag gaataccagc ctttggaact gaagaaaaaa
     5761 tccgtccgtg ccgccctccc cgcagtaaaa acaaaggtgg aagaatatct ctcgcaacat
     5821 aagaatgacg atgtcaatga aacctttgta aagaatttaa tagagtacat caataaagga
     5881 taacggtcct gcttaacgct gtattaacaa aaaagcgccg gtgcacgatt attgcataat
     5941 agtgtcctcc ggcgcaatct ttttataact ctacaggttt tttttatttc ttgcagcatc
     6001 ccgcattatt ttattgtcaa actatttacc taatattggc gaaatttaac tatttgataa
     6061 gttaagtttt ggccgagtag agagttaaca ttaagtcaca caaaacctgt aaacatgagg
     6121 aaacttttga ccttgtttgc ggtagccatg ctattttctg tcctggctat tgcacaaaca
     6181 aaaaccttat ccggaaaaat caccgatgca aaagggcaac ctgtaccctt tgccaccgtc
     6241 cggatcaagg gtaccaaagc cggagtaagt gcggatgcag acggtaacta cagcatcaaa
     6301 gtatctccct cccaaacatt gatcgtcaca ggcgccggta tggcgcctac ggaagtcgtc
     6361 gcccccgatg ctcccacctt caacatcacc gttgccatga aggacgccag catgaccgaa
     6421 gtagtggtca ccgccctggg tatccgcaga agcaagaatg agctgcccta ctcggtacag
     6481 cgcatcggcg gtgacgaggt gagcaaggag cgcagcggca acttcattac caacatggaa
     6541 ggcaaggtct ccggcgtgac catcaaccag gccaacacgc tgggcggttc gaccaacgtc
     6601 atcctccgcg gctacaaatc ggcgctgggc gataaccagg cgcttttcgt tatcgacggt
     6661 gtacccatcg ataataccaa cacgacctcc atcaaccagg taccgggaac ccagggaacc
     6721 cagtcttccg ggctcggtgg ctatgactat ggtaatgccg cggccgacat caaccccgac
     6781 gacatcgcct ccatcagcgt gctgaaagga gctgcggcca gcgcactcta cggggagcgc
     6841 gggttcaacg gcgtcatcat tattactacc aagaaaggcc gcaaaggcct aggtatcacc
     6901 gtcaatggcg gtgtcaccac cggcgctatc gacaaaagca cttttgctaa ataccagcat
     6961 acctatggcg ccaactatgg ttcggccaac ggatatggtt cacccgacgg caacttcctc
     7021 tatttcgatg tgctgggtaa cggcaccaac gacctggtaa cgccgaccac ggaggacgcc
     7081 tcctggggcg ccaagttcga tcccaacctg atggtttacc aatgggaagc cttcgataag
     7141 acttccagca cctaccacaa ggccacgccc tgggttgccg cccagcatga tcccaccacc
     7201 ttctggacca agccgttctc ctataacggt agcgtgttca tcgaaggcgg taacgacaaa
     7261 agcaccttca ctgccggcta cacccgcaac agcgatcgcg gcatcctgcc caatagccag
     7321 atcctcaagg atatggtcaa cacctccggc acgctgaaca tcaccgacaa actgacggcc
     7381 ggcgccagca tcaactttac caatatcagc ggcctcggcc gttatggcac cgggtatgcc
     7441 tccaataacg aagccaccaa cttccgcgaa tggt
//
LOCUS       DATGFG010000903         3283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_961759, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000903 DATGFG010000000
VERSION     DATGFG010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961759"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VKQ52_17110"
     CDS             <1..736
                     /locus_tag="VKQ52_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88980.1"
                     /translation="EGHRISLTRSSFFFSNRLTHRLSKKYGLQSNFLALTADQPAEKI
                     QYGNSFLLPAINRVLEEVVPNYRYTNLPELNAILRQYNVEASRGKEHTLTYRNKGLHY
                     HPLKADGTPAYEYVPARLFPSKPTLENLEKRFAENQSLRNTHRERLATLVGYTLAGSG
                     LSLEGLKQALAKKQVTLFVRPGKEPGAEQLWFIDLQNKTVFEGAKLGKDYSFTSVQKQ
                     LISEEAYQKQQETQRQNQHQRYRHSF"
     gene            747..2690
                     /locus_tag="VKQ52_17115"
     CDS             747..2690
                     /locus_tag="VKQ52_17115"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YWFCY domain-containing protein"
                     /protein_id="HLZ88981.1"
                     /translation="MNPPNAYSYKNITHLSFAISITCLGLNCYYAGYWAFEKWSLTSR
                     ITDRVLQVVQNTGLLQGFTIHGMIVFFLALSLTGAPARKSQSVTRRGVLIPLSVGALL
                     FTTAPLVFARVGRPLTTILVYFSFTITGYLLLLTGGSRLRRMLRLPWAKGDPFGKKRS
                     GFPQEQRRIDHDLSLNLPAEYDFNGKTMESWVNLVNPRRGILILGGSGSGKSRFIIEP
                     LIRQLMAKGTALFLYDYKYPALTNLVLRTFKAQRDQYPARTSFYCIQFQDLSRSHRCN
                     LLDPATLEWVSDALGAARTILLSMNKSWISRQGEFFVESPVNFLGALIWYLKKYAGGR
                     YCTLPHAVELAQVPYEKLFPILEAEPEIESLVRPFVEAHSNKSFEMLDSQITSARVPL
                     SRLASPDIYYILSGNDLSLEVNDPAAPKILCLGGDPKRQEALAPVLSLYIDRLNRLCN
                     RPGRHPCALVCDEFATVRAHSVTTTIATARSNDIIPILAVQDLSQLRVQYTRDEADLF
                     LNICGNLLCGQVGGETARWVSERFPKLQREQAGISTNSTDTAENLSLQWEPTVTPATI
                     AGLSSGEFVGILSDEPGRELELKAFHSRLILEPTAGSDSPPVELPPVHEVDEKTILQQ
                     FTQVRADIANLVTAGHKKLNRRP"
     gene            complement(2694..>3283)
                     /locus_tag="VKQ52_17120"
     CDS             complement(2694..>3283)
                     /locus_tag="VKQ52_17120"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HLZ88982.1"
                     /translation="FNSACVVLAVIAILALRRQQPTRQFRWLSGSSAIALFALIGWFM
                     VSAFRAPVVEKTGLEGRLMPSIPLQLTDSTAWLNTDEIPSGKPFIVMGFSPWCIHCQA
                     LTVDIKKHINEFKDTRIYYVTPDWFKNMKIFYRFYKLSQYPNFVMGRDSANLLFHYFK
                     TNKTPLIAIYDAKKRLKRVIPGQPTVAQLLEGIDN"
BASE COUNT          746 a         1022 c          838 g          677 t
ORIGIN      
        1 ggaaggtcat cgcatcagtc tcacccgttc atcctttttc ttttctaatc ggttaaccca
       61 tcgattatcg aagaaatatg ggctacagtc gaacttcctg gctttgactg ccgatcaacc
      121 cgcggaaaag attcagtacg gtaacagctt tctgctcccg gcgataaacc gtgtcctgga
      181 agaagtggta cccaactacc gttacaccaa tctcccggag ctgaatgcca tattgcgcca
      241 atacaacgtc gaagcaagcc gcggaaaaga gcacaccctc acctaccgga acaaaggcct
      301 gcattatcat cccctgaaag ccgacggcac gcccgcctat gaatacgtac ccgcccgtct
      361 gtttccatca aaaccaacgc tcgaaaacct ggagaaacga ttcgccgaga accagtctct
      421 ccgcaacacc catcgtgaac gcctcgccac ccttgtcggc tatacgctgg cgggttcggg
      481 cctcagcctg gaagggctca aacaggccct cgcaaaaaaa caggtaaccc tctttgtccg
      541 gccgggaaaa gaacccggcg ccgaacaact gtggttcatc gatctccaaa acaaaaccgt
      601 cttcgaaggc gcaaagctgg gcaaggacta ctccttcact tccgtgcaaa aacagctcat
      661 ttctgaagag gcctatcaaa aacagcagga aacccagcga caaaaccagc atcaacgcta
      721 tcgtcattca ttctaaaaac aacgttatga atccgccaaa cgcatattcc tataagaaca
      781 tcacccacct cagcttcgct atcagtataa cctgccttgg tctgaattgt tactatgccg
      841 gctattgggc attcgagaaa tggagcctca cgagccggat tacggatcgt gtcctgcagg
      901 tagtgcaaaa caccggcctg ctgcagggat ttacgattca tgggatgatc gtcttcttcc
      961 ttgccctatc gctcaccggc gccccggcca ggaagagcca gtccgtcacc cgccgcggtg
     1021 tcctgatccc tctctcagtc ggcgccctct tgtttaccac agcccccctc gtctttgcac
     1081 gggtgggaag gccgctgaca acgatcctgg tctatttctc tttcacgatc acgggctatc
     1141 ttctactact cacgggtggc agccgcctgc gccggatgct ccgtctgcct tgggccaagg
     1201 gcgacccttt cggaaaaaag cgatcgggct ttccccagga gcagcggcgt atcgaccatg
     1261 acctatcgct caacctgccc gcggaatatg acttcaatgg gaaaacaatg gaaagctggg
     1321 tgaatctggt caacccacgc cgcggcatcc tcatcctggg tggctcgggt tcgggtaaat
     1381 cccggttcat aatcgagccc ctgatccggc aattgatggc gaaggggacg gcgctctttc
     1441 tctatgatta taagtacccg gcgctcacca acctcgtcct ccggaccttc aaggctcagc
     1501 gcgaccaata tccagcccgg acaagcttct attgcattca attccaggat ctttcccgga
     1561 gccatcgctg taatctgctc gacccggcta ccctcgaatg ggtgtccgat gccctggggg
     1621 ccgcccggac cattttgtta agtatgaata aatcctggat cagccgccag ggcgaattct
     1681 tcgtcgagtc gccggtgaat ttcctggggg cgctgatctg gtatctgaag aaatatgctg
     1741 gcggacgtta ctgtacactc ccccatgctg tcgagctggc gcaggtgccc tatgaaaagc
     1801 tgtttcccat cctggaggcc gagcccgaga tcgaatccct cgtccgaccc tttgttgagg
     1861 cccacagcaa taaaagcttc gaaatgctgg acagccagat cacttctgcc cgcgtcccgc
     1921 taagccggct cgcctcgccg gatatttatt atatcctttc gggcaacgac ctaagcctcg
     1981 aggtcaacga ccccgcggcg ccaaagatcc tctgcctggg tggggacccg aagcggcagg
     2041 aagccctcgc cccggttctg tcactttata tcgatcgcct gaacaggctc tgcaaccgtc
     2101 ccggtcgcca tccctgcgcc ctggtctgcg atgaatttgc cacggtccgc gctcacagcg
     2161 tcaccacgac catcgccacc gcccggtcga acgacatcat tccgatcctt gccgtccagg
     2221 atctcagcca gctccgggtc cagtacaccc gcgacgaagc cgatctcttt ctcaacatct
     2281 gcggcaacct cctctgcggc caggtgggcg gcgaaacggc gcgttgggtc agcgaacggt
     2341 tcccaaaact gcaacgcgag caggccggca tctccaccaa cagcaccgac accgccgaaa
     2401 acctgtcgct gcaatgggag cccacggtta cccccgccac catcgccggt ctctcctccg
     2461 gcgaattcgt aggcatcctc tctgacgagc ctggccgcga gctcgagctc aaggcctttc
     2521 atagccggct gatcctcgag cccaccgccg gcagcgactc cccgcccgtc gaactaccgc
     2581 ctgtccacga ggtggatgaa aagaccatct tgcagcagtt cacgcaggtc cgcgcggaca
     2641 tcgcaaacct ggtaacggcc ggacacaaga aattgaatcg caggccgtag cagtcaatta
     2701 tcaatacctt caagcaattg tgcgaccgtg ggctggccgg ggatcaccct tttcaatctt
     2761 tttttcgcat catagatagc gatcaggggc gtcttgttgg tcttgaaata gtggaagagg
     2821 aggttggcgc tatcgcggcc catgacaaag ttggggtatt gggaaagctt ataaaatcgg
     2881 tagaagatct tcatattctt gaaccagtcg ggggtgacat agtagatgcg ggtatccttg
     2941 aactcgttga tatgcttttt gatatcgacc gtcagcgcct ggcaatggat gcaccagggg
     3001 gaaaacccca tcacgataaa gggtttcccg ctcgggatct cgtctgtatt cagccacgcc
     3061 gtgctatccg taagctggag ggggatggaa ggcatcaggc gaccctcgag cccggtcttt
     3121 tcgactacag gcgcgcggaa ggccgagacc atgaaccagc cgatcagcgc aaaaagggcg
     3181 atggccgagg agcccgataa ccatctgaac tgccgggtag gctgctggcg gcgcagagcc
     3241 agaatggcaa tcaccgcgag gacgacacag gcgctattga agg
//
LOCUS       DATGFG010000904         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1668652, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000904 DATGFG010000000
VERSION     DATGFG010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668652"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VKQ52_17125"
     CDS             <1..616
                     /locus_tag="VKQ52_17125"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ88983.1"
                     /translation="TGLLNGRSCTTHWEFAHSFREMYPDVNLVEDKIITDENNIYTSG
                     GAYSWLNLILYLVEKYCGREIAVTCSKGFQVDFQRVSQSPFIIFTGQKDHEDVAVQAA
                     QDFIEKNTHCKITVDELAGRLILGRRNLERRFKRATGNTVMEYIQRVKMEAVKKSLET
                     SRLGVNEVMDRVGYTDPKAFRMIFKKVTGLSPLQYRNRYNREYA"
     gene            complement(619..1287)
                     /locus_tag="VKQ52_17130"
     CDS             complement(619..1287)
                     /locus_tag="VKQ52_17130"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HLZ88984.1"
                     /translation="MTSIGNDIVALDHGNADRSGQYVFYSKVLTAFEKELYSQERFPG
                     LSFAHFVWLCWSIKEASFKCIKRNQPHLLFSPQKINIKALEPLPEPFLLKDGNCDWDI
                     AAPETAFTGYCGTVGFGDMVVTAISRVYDTFISTIATVDAGSDTLCWGIRRIARTDPA
                     HQSESVRLFVLERLRRSCPEDEIKIEKASAGYPVIVRNAEWTNMPLSFSHHHDLVAFS
                     FLHP"
     gene            complement(1284..1541)
                     /locus_tag="VKQ52_17135"
     CDS             complement(1284..1541)
                     /locus_tag="VKQ52_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88985.1"
                     /translation="MDRQEILEELKKVLTPYTVNKEVLNSLTEQTDLIKDLKINSANL
                     VDIIIDAEAKYDIEIDMDSAEKIHSVGNCIDVILEKLNAPN"
     gene            complement(1545..>2100)
                     /locus_tag="VKQ52_17140"
     CDS             complement(1545..>2100)
                     /locus_tag="VKQ52_17140"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88986.1"
                     /translation="AGALVLEDLEDALARNATIYAEILGGVNNSGGQRAGGSMTAPSP
                     AGIVSCIRGALDKAGISGSEVDLVCGHLTATAADKLEIECWASALGRKGADFPLINSL
                     KSMIGHCLSAAGSIEIVAVVLQLWRQFVHPNVNLEDPHPEIVDLISPESMPTELINKT
                     LNIVAKANFGFGDVNACLIFAKHR"
BASE COUNT          496 a          540 c          558 g          506 t
ORIGIN      
        1 cacggggctg ctcaacgggc gaagctgtac gacgcactgg gagttcgccc atagcttccg
       61 ggaaatgtat cccgatgtca accttgtgga agacaagatc atcacggatg agaacaacat
      121 ctataccagc ggcggcgcct attcctggct caacctgatc ctttatctcg tcgagaaata
      181 ctgtgggcgg gagattgcgg tcacgtgctc caagggtttc caggtggact tccagcgggt
      241 gagccagtcg ccctttatca tctttaccgg tcagaaggat catgaggacg tggccgttca
      301 ggcggcgcag gacttcatcg aaaagaatac gcattgcaag atcaccgtcg acgagctcgc
      361 cgggcggctg atcctcggtc gccggaacct ggagcgccgg ttcaagcggg ccacgggcaa
      421 taccgttatg gagtatatcc agcgggtgaa gatggaggcg gtgaagaaga gcctggagac
      481 ctcgcggctg ggggtgaatg aagtgatgga cagggtaggg tacaccgatc caaaggcgtt
      541 ccggatgatc tttaagaaag tgaccgggct ttcaccgctg cagtaccgca accggtataa
      601 ccgggagtat gcgtaagcct aaggatgaag aaaagaaaag gcaacaagat cgtgatgatg
      661 cgagaaggag agcggcatat tcgtccattc ggcatttctt actatcaccg ggtaacccgc
      721 gctggctttc tctatcttaa tttcatcttc cggacaggat cgccgcagtc gctcgagaac
      781 aaaaagccgg acactttcgg attgatgggc gggatcggtc cgggctatcc ttcggatacc
      841 ccaacagagg gtgtcgctac cggcatccac cgttgcaatc gtcgagatga aggtatcata
      901 taccctggaa atggccgtta ctaccatgtc gccaaaacct acggttccgc aatagccggt
      961 aaacgccgtc tccggggcgg cgatgtccca atcgcaatta ccgtctttca acaggaaggg
     1021 ttcgggaagt ggctccaggg cttttatatt tattttttgg ggggagaaaa ggagatgagg
     1081 ttggttcctt tttatgcatt tgaaggaggc ctctttaatg gaccagcaca gccaaacaaa
     1141 atgggcaaag gataatccgg gaaaacgctc ctgtgaatac aattcttttt caaaagcggt
     1201 caaaactttg gaataaaaga cgtattgtcc gcttcggtcg gcattgccat gatccagggc
     1261 gacgatatca ttcccgatac tggtcaattg ggcgcattga gtttctccag gatcacgtcg
     1321 atgcaattgc ccacactgtg gatcttttcg gccgaatcca tatcgatctc gatatcgtat
     1381 ttagcttccg cgtcgatgat aatatccacc aggttggctg aattgatctt cagatccttt
     1441 atcaggtctg tttgttcagt taaggagttt agcacttctt tgtttacagt atagggtgtc
     1501 aatactttct ttagttcttc cagaatttct tgtctatcca ttttttatcg gtgtttagca
     1561 aaaattaaac aggcgtttac gtccccaaag ccaaaattag ctttggcgac aatattaagg
     1621 gttttattta taagttccgt gggcattgac tccgggctta tcaggtcaac gatctccgga
     1681 tgcggatctt ccaaatttac attcgggtgt acgaactggc gccagagctg caataccacg
     1741 gccacgatct caatggaccc tgccgcgcta agacaatgtc cgatcataga cttcagggaa
     1801 ttgatcaggg gaaaatccgc ccctttcctg cccagcgcgg aggcccagca ttcgatctcc
     1861 agtttatcgg cggcagtagc cgtcaggtgg ccgcatacca gatccacctc cgacccgctt
     1921 atcccggctt tgtccagcgc cccccggatg caactgacaa ttcccgccgg actgggcgcg
     1981 gtcatcgatc cacccgcccg ttggcctccc gagttattga cgccgcccaa tatctccgca
     2041 taaatagtgg cgttccttgc cagggcgtct tcgaggtctt ccagcaccag ggcgccggca
//
LOCUS       DATGFG010000905         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1418281, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000905 DATGFG010000000
VERSION     DATGFG010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418281"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VKQ52_17145"
     CDS             complement(<1..737)
                     /locus_tag="VKQ52_17145"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HLZ88987.1"
                     /translation="MANIKERFKDKSDGLAAEIKEIIKEHGNKKIGEVTLGQIYQGMR
                     GITGLVTETSLLDAQEGIRFRGFSIPELQQRLPKAQGGTEPLPEGLFHLMLLGELPTE
                     EDVHDITANWQRRSHVPNHVFAAIEALPKDSHPMTQFVVAIMALQTESKFSERYAKGM
                     AKKDYWEAVFDDSMDLIARLPRVAAYIYRRKYKNEDHIHPDGLLDWAGNFAHMLGYED
                     ETFRELMRLYMTIHADHEGGNVSAHTT"
     gene            complement(863..1957)
                     /locus_tag="VKQ52_17150"
     CDS             complement(863..1957)
                     /locus_tag="VKQ52_17150"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HLZ88988.1"
                     /translation="MIKLLDRYILKKFIGTFVFTMLVLTSIAVVIDTSEKADDFVRSG
                     LNAKQLIMLYYIGFIPFIMSMIYPLMVFIAVIYFSSKMAGRSEIIAILAGGVRFNRML
                     RPYLIGAVLLSLIFWFASQYFIPKANEIRTDFQAVYVDRNSSYNGDPYKNNNFYLRVD
                     PTTFVGFRYYDTLNKTATNFFMQKLRGNKVYYNVRAESVRWDPAKKDWKLTTVVERTI
                     DGLKETLKKLDTLHVNLNVQPKELRRDDYLKDKLTTPELQQFIHMEEVRGSEGLNTFK
                     VELYHRDATPFSVIIMALIGAIIASRKIRGGSGLHLAVGIVLAAIFVVMDRFAVTFST
                     KGDLPPMLAAWLPNIIFSGVACVLYVKTPK"
     gene            2125..3147
                     /locus_tag="VKQ52_17155"
                     /pseudo
     CDS             2125..3147
                     /locus_tag="VKQ52_17155"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(3461..>3986)
                     /locus_tag="VKQ52_17160"
     CDS             complement(3461..>3986)
                     /locus_tag="VKQ52_17160"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HLZ88989.1"
                     /translation="KASCAYVAGKGAVGNAIGGLSVGEPEQEMYDICQACCEELPVER
                     PRYLMGVGTPWNILECIDRGVDMFDCVLPTRNGRNAMLFTSKGVINIDNKKWEMDFSP
                     IDDGLDCEVSQFYSKAYLRHLVKSKEILALTIASVHNLAFYLHLVREARKHILEGDFA
                     SWKREQVEVLKQRL"
BASE COUNT          912 a         1406 c          805 g          863 t
ORIGIN      
        1 tgggtcgtat gcgcactcac attccctccc tcatgatccg catggatcgt catatacagc
       61 cgcatcagct cccggaaggt ctcatcctcg tatcccagca tatgggcaaa gttccccgcc
      121 cagtccagca acccgtcggg atgaatatga tcctcgttct tatattttct ccggtagata
      181 tacgccgcaa cccggggcaa tcgcgcgatc aggtccatag aatcgtcaaa aacagcctcc
      241 cagtaatcct tcttcgccat ccccttcgca taccgctccg aaaacttgct ctccgtctgc
      301 aaggccataa tggctacgac aaactgcgtc atcggatggg aatccttcgg taacgcctcg
      361 atcgccgcga aaacatggtt cggaacatga ctgcgccgct gccagttggc ggtaatatcg
      421 tgtacatcct cctccgtggg caattcaccc aacaacatca ggtggaataa cccctcgggc
      481 aacggctccg tcccgccctg cgccttcggc agccgctgct gcagctcggg aatggaaaaa
      541 ccgcggaaac ggatcccttc ctgcgcatcc agcaacgacg tctccgtcac caggcccgtg
      601 atcccccgca tgccctggta aatctgcccc agcgtcacct ctccgatctt cttatttcca
      661 tgctccttga tgatctcctt tatctcggcg gccagcccgt cggacttatc cttgaaccgt
      721 tcttttatat ttgccatatg ccctggattt atctgtaaaa gtactgtaca aaacgttggg
      781 gagcaaatca accgttgtct ttaaacgtat tttaatattc gccgaaggcc ctcctggcca
      841 tccagtacaa ttaaccccat ccctacttcg gcgtcttcac atacagcaca caggccaccc
      901 cgctgaaaat gatattcggc aaccacgccg ccagcatcgg cggcaggtcc cccttcgtcg
      961 agaacgtaac ggcaaacctg tccatcacca caaatatcgc cgccagcacg atccccaccg
     1021 ccaggtgcaa cccactaccc cccctgatct tgcggctggc aatgatcgcc ccaatcagcg
     1081 ccattattat aacagagaag ggagtcgcat cccggtgata aagctccacc ttgaacgtat
     1141 tcaacccctc cgacccccgc acctcctcca tatggataaa ctgttgcaac tccggcgtcg
     1201 tcagcttgtc cttcaggtaa tcgtcccgcc gcaactcctt cggctggaca ttgagattga
     1261 catgcagcgt gtccagcttc ttcagcgtct ccttcaatcc atcgatcgtc cgctcgacca
     1321 ccgtcgtcag cttccagtcc tttttcgccg ggtcccaccg caccgactcc gcccgcacat
     1381 tgtagtagac cttgttcccc cgcaatttct gcataaagaa attggtcgcc gtcttgttca
     1441 acgtatcata ataccggaat cccacaaacg tagtggggtc cacccgcagg taaaaattat
     1501 tgttcttgta agggtccccg ttgtaggagc tgttccggtc cacatacacc gcctggaaat
     1561 cggtccgtat ctcgttggcc ttgggaataa aatactggct ggcaaaccaa aagatcagcg
     1621 aaagaaggac agccccgata agataaggcc gcaacatccg gttgaaccgc accccacccg
     1681 ccaggatcgc gatgatctcc gaccgccccg ccatcttgct gctgaaataa atgacggcga
     1741 taaacaccat caggggatag atcatcgaca tgataaacgg gatgaacccg atataataca
     1801 gcatgatcag ctgcttcgca ttcagcccgc tccggacgaa gtcatccgcc ttctcgctcg
     1861 tatcgatcac caccgcaata gaggtgagta ccagcatcgt aaaaacgaat gttccaataa
     1921 atttttttaa tatgtatctg tcgagcagtt ttatcatatc tacgcgccgc aaaaataggt
     1981 catatcaccc acccggggta taaaaattgc tgttaataat cggccggcac cccccttact
     2041 gccagcctgc cacaaaccgc cccatcccaa atctcccgat tgctgccccc cctcccccaa
     2101 tccatcacct taaaaactga tccaatgcac ttcatctgga caaccctctc ctatgcctac
     2161 ctggccctcc aggtcctcct cgcctcctgg ctggtccagc ccctgatcct gctcctggtc
     2221 tacggcatca ccaggctcct cggcatccgg ccacacccca ccccattcca ttccgttaca
     2281 agggactacc agttcggcat cgtcatcacc gcccaccgcg aaacggcctt cctcccaccc
     2341 atcatcgact ccctcctgaa acaaacccac acccggttca acgtctatat cgtcgccgat
     2401 gattgcgaca ccagccgcct ggtcttccgc gacccccgca tccatatact ccggccaccg
     2461 gtccccctcc acgatcaact ggcctccctc gatttcggct ggcgccacct cgaccggcag
     2521 gacgaagtgc tgatcatctt cgatcccgat aacctggtac atcccgactt cctccgcacc
     2581 ctcaatgcct ggtacaacag cggctatcgc gccgttgtcg gtcatatgca gtccaagaac
     2641 agggaaacca cctatgcgca gatcgacggc tggggcgcct ccctgagcaa ttttatggag
     2701 cgggatatgc gttccctcct cggactgtcc ccaaacatct ccggcagcgg catctccgtt
     2761 cacaaagaag tatacgatat cattcactat gatcgccgca gcaggaccgg cggcttcgat
     2821 aagcaattgc aaatggggac ggtaaaagcc gtgcaccgca tcgcctacgc ccgggacgct
     2881 ctcttctatg acgaaaaggt cggcgacagc cacaatttcg aacggcagcg catccgctgg
     2941 atagccgccc atttcaaatt cctggggaat gctttccact tattgttgac gggaatccgc
     3001 aaacgcgacg gcaacctggt ctatttcggc tataatctca tccgtccgcc ctggttcatc
     3061 ctgctgaccc tgtcgttcct gatgatggcc accgactggc tcatcgatcc ccggctgagt
     3121 atcgcctggc tggcatgctt cgccgccttt agcctgtcct tcctcctcat cgtcgcaaag
     3181 gacatcagca acaaatcccc ggtaaaagcg ctggcaaata ttccccgcat cttctaccgc
     3241 cagttccggg cgctcctgcg tctccggctg aacaaaacat ccctcctcaa aacaacccat
     3301 tccgaactcg tctacatcga cgacatcatc cgcccccagg cccagccgca accgcccgca
     3361 aacctcaccc catcctccgc aaacttcgct cgtcccccca tcaaccacaa tccatccccc
     3421 gcaaacttcg ctcaaccatc cgccaacctt gtccatcccc ctacaaccgc tgcttcaaca
     3481 cctccacctg ctcccgcttc cacgacgcaa aatccccctc caggatatgc ttcctcgcct
     3541 ccctcaccag gtgaagataa aaagccagat tatgcacact cgcgatcgtc aaggccaata
     3601 tttccttcga cttgacgaga tgccgcaaat aggcttttga atagaactga ctcacctcac
     3661 aatcaagacc atcgtctatt ggggagaagt ccatctccca tttcttgtta tcgatattga
     3721 tcaccccctt cgacgtaaaa agcatcgcat tccgcccgtt ccgtgtcggc aacacacaat
     3781 cgaacatatc caccccccgg tcgatacatt ccaggatatt ccagggcgtc cccaccccca
     3841 tcaaataccg cggccgctcc accggcaact cctcgcaaca ggcctggcag atatcataca
     3901 tctcctgctc cggctccccc acgctaagcc cgccgatcgc attcccgaca gcccccttgc
     3961 cggcgacata cgcacaggaa gccttg
//
LOCUS       DATGFG010000906         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_733122, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000906 DATGFG010000000
VERSION     DATGFG010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733122"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VKQ52_17165"
     CDS             complement(<1..1030)
                     /locus_tag="VKQ52_17165"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLZ88990.1"
                     /translation="MNHIVLDRICILNFKGIEKLQISFDPRSQNIWGENGTGKTTIAD
                     AFQWLLFGKNSLGEADFGIKRLDKDGKKVDKTDVEVEGTIDFNGRKITLKHRFIELWT
                     QQKGSEERKLTGNTHEYFVDNAPYKLAEYKAFINKLVDEAIFPMITNPLHFNRLAWQK
                     RREILLSISGDISNADLAAAYPEFKELVAQLSNKTLSKYKQEVVANKKLSKDQLEGLP
                     TRIDEVRRGMPVEQDYEAIVAQVQNREERLAEIDAELLNVSKASETFTQTVRDHQARV
                     RGLKLDQDGIASVLRYARKTEEAEAGRELAQKRTALDQTEKYLTDARNALDRLETRKG
                     AIRKEIDAG"
     gene            complement(1260..1703)
                     /locus_tag="VKQ52_17170"
     CDS             complement(1260..1703)
                     /locus_tag="VKQ52_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88991.1"
                     /translation="MAQQFKDQYARLGINDEVPTDPIQKAEVIYQAACRKLSLNPEDM
                     PDVSRVRGKYRASQLSFHKLEIIRDAIVGDREAKWNDYAEYKWGSWFYLNSPGFRFVD
                     SYYSCTNAVSGAGSRLCTFSKNDQDFFAIECIALWAEFHGGVLPE"
     gene            complement(1791..2018)
                     /locus_tag="VKQ52_17175"
     CDS             complement(1791..2018)
                     /locus_tag="VKQ52_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88992.1"
                     /translation="MNPLTFRLVAIAILLFWFFCVRGRVINWVGFFKVLPGILLDVII
                     QIGLSIGDLFRRKKYWHPVNPHVTDEKDLYD"
     gene            complement(2155..2331)
                     /locus_tag="VKQ52_17180"
     CDS             complement(2155..2331)
                     /locus_tag="VKQ52_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88993.1"
                     /translation="MFIVINESLVSAEFEVDLTDFERQVREIFKDDENKPVTPEWLER
                     KERVWQKIQKSLPI"
BASE COUNT          546 a          658 c          559 g          640 t
ORIGIN      
        1 ggccggcatc tatctccttc cggatagcac ctttccgtgt ctcgagcctg tcgagggcgt
       61 tccgagcatc ggtcaaatac ttctccgtct ggtcgagggc agtacgcttt tgagccaatt
      121 cgcggccagc ctcagcctcc tcggttttcc gtgcgtagcg aagcacactc gcgatgccat
      181 cctggtccaa tttcagcccg cggaccctcg cctggtgatc tcggacagtc tgagtaaacg
      241 tctcactggc cttgcttacg ttcaggagtt cggcatcgat ttccgctaac cgttcttccc
      301 gattttgcac ttgtgcaacg atagcctcat aatcctgctc tacaggcata cctcgtcgaa
      361 cctcatcgat cctggtcggc aaaccttcca actgatcttt gctcagtttc ttgttcgcga
      421 ccacttcctg cttgtatttc gagagcgttt tgttgcttag ctgggcgacc aactctttga
      481 actcggggta tgcggcggcg aggtccgcgt tgctaatatc cccgctgatc gacaaaagga
      541 tctccctccg cttctgccac gcaagccggt taaaatggag aggatttgtt atcataggga
      601 agatcgcctc gtcaaccagt ttgttgatga acgccttata ttccgccaat ttatacggcg
      661 cattgtcgac gaaatactcg tgcgtattgc ccgtcagttt acgttcctcg ctgcctttct
      721 gctgggtcca aagttcaatg aaccgatgtt tcaaggtaat cttgcgacca ttgaagtcga
      781 tcgttccctc gacctccacg tcggttttat caaccttctt cccgtccttg tctaaacgct
      841 tgatcccaaa gtctgcctcc ccaaggctat tcttgccgaa cagcagccat tggaaagcgt
      901 cggcaatagt agtcttgccc gttccgtttt ccccccaaat attctgactg cgaggatcaa
      961 aagatatctg caacttttct attcccttga agtttaggat gcatatcctg tctagcacga
     1021 tgtgattcat gtatatagaa attaatggtg tctcaaaaca gccgccagcg ttcactctgg
     1081 cggccggtac ctcacaaaca tcccccggtt aaccttgctg ggtgcctgcc tgagtctgct
     1141 ctgcagtctc agttccatcc ttgtcgccgc cggggcctac atggttcttc agttcatcgg
     1201 gatttctgtt cgtcatatat aaaaagctgc gtaaccgtcg cagcgggttt accacgacct
     1261 tattcaggca atacgccgcc gtgaaattca gcccacaatg caatgcattc gatggcgaaa
     1321 aaatcctggt cattcttcga gaatgtgcaa agccgggagc cggcgcccga gaccgcattc
     1381 gtgcacgagt agtacgaatc gacgaaccgg aagccggggg aattgaggta aaaccaactg
     1441 ccccacttgt actcagcata gtcgttccac ttggcttccc ggtcaccaac tatggcatcc
     1501 cggataatct ccaacttgtg aaacgagagc tgagaagccc gatattttcc ccgtacgcgc
     1561 gatacgtccg gcatgtcttc ggggtttaaa ctgagcttgc gacaagccgc ttggtagatt
     1621 acctcggctt tctggatggg atcggttggg acctcatcgt tgatgcccag gcgggcatat
     1681 tgatctttga actgttgcgc catgtatata ttttttaatt tgattttaaa aggagcccga
     1741 gcgtagagac gccgggcctt taacccctaa accctaaacc gtagaaagaa ttaatcatac
     1801 aggtccttct catcggtcac atgaggattc accggatgcc aatatttttt gcgtcggaaa
     1861 agatcgccga tactcagacc tatttgaatg ataacgtcca gaagaattcc gggcaacacc
     1921 ttgaaaaaac caacccagtt gatgacgcgc ccgcggacac agaagaacca gaagagaagg
     1981 atcgcaatag cgacgagacg gaaagtaagg ggattcatgt cgatggcttt taatagttac
     2041 gatgttgttc gtcagctttt cgcttggctg ctttcgcctg gatcatccac ttacgagcac
     2101 agccgtacat gaatacccag aaaaaacagg cggcgacgaa aatccatttg tagttcatat
     2161 gggtagggat ttttggattt tctgccacac tcgctctttg cgctccaacc attcgggggt
     2221 gacaggctta ttttcgtcgt ctttgaagat ctcccgaacc tgacgctcaa aatcagtaag
     2281 gtccacttca aattcggccg acaccaggct ttcattaatc actatgaaca tgcttcagga
     2341 ttttagcttt aaaaagtggc ccgggcttcg ccgccggttg ctacccgggc ctgaccatta
     2401 atc
//
LOCUS       DATGFG010000907         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_962273, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000907 DATGFG010000000
VERSION     DATGFG010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962273"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3056
                     /locus_tag="VKQ52_17185"
     CDS             <1..>3056
                     /locus_tag="VKQ52_17185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ88994.1"
                     /translation="DAASLATNYVKVLCEDEDHQIWVGTVGGGLDQLERPTGTFRHYA
                     HDARDPRSINNNSISSILEDSRHRLWVGTYTGLDLFDRAAQGFVHYRKDNHPKDNHSS
                     VGITNNTINSLAEDGDGNLWIGTENDGLVVLDSMGVSHHFLHDDVDDASIGTNSLYAL
                     YRDSKDNMWVGSFAGGLDFVSSDTRKFPVYRHSSSPYSLSDNRILCIYEDSRNNVWIA
                     TDGGGVNLFDRKTERFTHYRHNQDDPRSICGDYILKVTEDSKGNLWFGSWADGLTIYN
                     RRNNTYTNLKADPDNPHGLSSNNVWAICEDQQHRMWLGTYGGGLDLYDPVRRWFVHYR
                     HNDDDPRSLANDKIHSVLVDSKGRLWIGMDGGGVDLFDPLTGTFAHYRHADGKNSLCG
                     DYVNNIIEDREGDLWISTTEGLSRYRVNANLFTSYTVKDGLPDEVIFGVIEDSTGSLW
                     ISSNKGICHWDMAHRRLKNYGVADGLQSNEFKEQAYCRTRSGLLFFGGVNGFNIIDPK
                     NVKTVSFDPPLVMTSFQVFNREVPIGGNGKNPSPLSKAISETESIVLPYKSSVFSFLF
                     ASLNYTTPDKKQYAYMLEGFDKSWNYVGAERSATYTNLDPAKYVFKVKGLTNDGEWSE
                     RVLSLQVQITPAFWMTWWFRVLLVLIIIGTGIVFYRLRINTMNSLTRELERQVKERTE
                     RLTSLTLEERKARQEAEHANKAKSIFLATMSHEIRTPMNGVIGMASLLAETPLTSQQR
                     EYNSTILSCGESLLSVINDILDYSKIDSGKMEIEQQPFDLRKCIEGVLGLFYEKATKS
                     GLQVRYHIGAEVPPYIVGDALRLRQILMNLVSNAVKFTHEGEIFLRVHLLKEDPSGVI
                     HVCFEVKDTGIGIPEEKIGILFKSFSQVDSSTTRKYGGTGLGLAITEKLVSLMGGQIT
                     VTSRPGQGTVFSFTILSRPGEGAMVQEQEGGGGQERMSTDFAESFPLRILVAEDNIIN
                     QQLIRQILGNLGYAPDCVENGALAVAAVEEKEYDLILMDVQMPEMDG"
BASE COUNT          660 a          696 c          996 g          704 t
ORIGIN      
        1 gatgctgcga gtcttgcgac taactatgtc aaggtgcttt gcgaggatga ggatcaccag
       61 atctgggtcg gaacggtcgg cggcggcctc gatcagctgg agcggcctac cggaaccttc
      121 cggcactatg cgcatgacgc gcgcgatccg cgatcgatca acaacaattc gatctcttca
      181 attttggagg acagccgtca ccggctttgg gtggggacct atacggggct cgatcttttt
      241 gaccgggcgg cccaggggtt tgtccattac cggaaggata accacccgaa ggataaccat
      301 tcgagtgtag ggatcaccaa taataccatc aattcgctgg cggaggatgg ggatggcaat
      361 ctgtggattg ggacggagaa tgacgggctt gtcgtcctcg attcgatggg ggtctctcat
      421 cattttttgc atgatgatgt ggatgacgcc agtatcggaa ccaattccct ttatgcgctt
      481 taccgggatt ccaaggataa tatgtgggtc ggctcttttg ccggcgggtt ggattttgtc
      541 agttcggaca cccggaagtt cccggtttac cggcacagct cttcccctta tagtcttagt
      601 gataaccgga tcctttgtat ctacgaggat tcgcgtaaca atgtgtggat cgccacggat
      661 ggtggcggcg tcaatctttt tgaccggaag acggagcggt tcacgcatta ccgacataac
      721 caggatgatc cccggagtat ctgtggggat tatattttaa aggtaaccga ggatagcaag
      781 ggaaatctgt ggtttggcag ctgggccgac gggttgacga tctataatcg ccggaacaat
      841 acgtatacca atctgaaggc ggaccctgac aatccgcatg gattgagcag caacaatgtc
      901 tgggccatct gcgaggacca gcagcaccgg atgtggctcg gcacctatgg cggcgggctc
      961 gatctttatg atcctgtgcg ccggtggttc gtccattacc ggcacaatga cgatgatccg
     1021 cgaagtcttg ccaatgacaa gatacattcg gtactggtgg acagcaaggg acggttatgg
     1081 atcgggatgg atggcggcgg ggtcgatctt ttcgatccgc tgacggggac ctttgcgcat
     1141 tacaggcatg cggacggcaa gaacagtctt tgcggagatt atgtcaacaa tatcatcgag
     1201 gaccgggagg gtgatctctg gatcagcaca acggaggggt tgagccgtta ccgcgtgaac
     1261 gcgaacctgt tcacctccta taccgtcaag gatgggttac cggatgaggt gatctttggg
     1321 gtgatcgagg attcgaccgg gagcctttgg atcagctcca acaagggcat ctgccactgg
     1381 gatatggcgc atcgccggct gaagaattat ggcgtggcgg atggtttgca gtccaacgaa
     1441 ttcaaggagc aggcgtattg caggacccgt tcgggactct tgttctttgg cggggtcaac
     1501 ggattcaata tcatcgaccc gaagaatgtg aagacggtgt cattcgatcc gccgctggtg
     1561 atgacgagct tccaggtgtt caaccgggag gttcccatcg ggggtaacgg gaagaacccg
     1621 tcgccgttga gcaaggccat ctcggagacc gagagtatcg tgctgcccta taagagcagt
     1681 gtgttctcct ttctttttgc ctccctcaac tatacgaccc ccgataagaa gcagtatgcc
     1741 tatatgctgg agggattcga caagagctgg aactatgtgg gcgccgagcg gagtgcgacg
     1801 tacacgaatc tcgatccggc caaatatgtc ttcaaggtga aggggctgac caacgacggg
     1861 gagtggtcgg agcgggtgct tagcctgcag gtgcagatca cgccggcgtt ttggatgacc
     1921 tggtggttcc gggtgctgct ggtgctgatc atcatcggca ccggtattgt tttttaccgg
     1981 ctccggatca atacgatgaa ttcgctgacg cgggagctgg agcggcaggt aaaggagcga
     2041 acggagcggc tgacgagtct tacgctggag gaaaggaagg cccgccagga ggccgaacat
     2101 gcgaataagg cgaagagtat cttcctggcg acgatgagcc atgagatccg gacgcccatg
     2161 aatggggtta tcgggatggc ttcgctgctg gcggagacgc cgttgacttc gcagcagcgg
     2221 gaatacaatt ccaccattct cagttgtggg gagagcctgc tgagtgtcat caatgatatt
     2281 cttgactatt cgaagatcga ttcgggtaag atggagatcg agcaacaacc ttttgatctg
     2341 cggaagtgta tcgagggggt gcttgggctt ttttatgaga aggccacgaa gtcggggttg
     2401 caggtgcggt atcatatcgg cgccgaggtt ccgccttata tcgtggggga tgcgttgcgg
     2461 ctgcgacaga tactgatgaa cctggtgagc aacgccgtga agttcacaca tgagggggag
     2521 atctttttgc gggtgcattt attgaaagag gacccttcgg gggtgatcca tgtttgtttc
     2581 gaggtgaagg acacgggcat cggtatcccg gaggagaaga tcggtatcct gttcaagtct
     2641 ttttcgcagg tggattcctc cacgacccgg aaatatggcg gcaccgggct tgggctggcg
     2701 atcacggaga agcttgtctc gctgatgggt gggcagatca cggtgacgag ccggccgggg
     2761 cagggtacgg tgttcagttt tacgatcctt agccggcccg gggagggtgc gatggtgcag
     2821 gaacaggagg gtggcggcgg acaggagcgg atgagtacgg attttgcgga atcctttccc
     2881 ttgcggatcc ttgttgcgga ggacaatatt atcaaccagc agttgatccg gcagatactg
     2941 ggtaatctcg ggtatgcgcc ggattgtgtg gagaatgggg cgttggcggt tgccgcggtg
     3001 gaggagaagg aatacgacct gatcctgatg gatgtgcaga tgccggagat ggatgg
//
LOCUS       DATGFG010000908         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_617573, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000908 DATGFG010000000
VERSION     DATGFG010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617573"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VKQ52_17190"
     CDS             complement(<1..186)
                     /locus_tag="VKQ52_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88995.1"
                     /translation="MTVYYCYDAYCGWCYGFSPVIRRIYEEYRSVLAFEVLSGGMILP
                     GKPQHIGVMAEYIRSAYP"
     gene            complement(246..2564)
                     /locus_tag="VKQ52_17195"
     CDS             complement(246..2564)
                     /locus_tag="VKQ52_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ88996.1"
                     /translation="MRSIRTIFSMLLAAVLLMGTALAQDGPPPQQQQQQQQEWPRSFT
                     TDDGTVIKIYEPQPESFADNILKSRWAISVLQPGKTDPVFGTFWSVANVETDRDNRRV
                     IIQSVKVPNVKFPGQTDDNFTNSLKTTLETNLPQAAGDLSLDELLSSLDENTEQKKLS
                     KDLNTSAPRIIYSDHPALLVMIDGQPKLQNNKDWNLDVVVNTPFTIVKANNGGFYLYG
                     GKHWYQAQSATGPYSPAGNVPSELDQVRSAVDNANSSNAGYIDSAGAAQDNKVSDIIV
                     STSPAELIQTDGKPSFTAIPGTGLSYASNSTNDIFQDQGSGKYFVLISGRWYSSGSLT
                     GGWHYVASNSLPGDFAKIPEGSPKDNVLASVAGTDAAREAIMDAQVPQTAKVDRNSAN
                     TSVNYNGEPQFSPLQGTDMQYATNTSSSVMLDHGIYYTVDNGVWFQSPSPNGPWTVAT
                     ERPDEVDRIPPTSPMYNTKYVYIYDVTPDYVYMGYTPGYLNTYIYGPTVVYGTGFYYD
                     PWFNGFYYPRPWTWGFNVCYNPWAGWTLGYGFGFGWFHYGLGFHGGWWGGWAGGGWWG
                     PHYYRPGYAWNRYRSYGFYGANYYRNRNVYVNNYHTNIYAGRGGVITRDNHSFYNRTS
                     FGRGASFGNAGRPGFNGAGRPVGPQGGRPGGANGGGFARPGANNVFSDRSGNVYQRNQ
                     ATNQWQQRSNNNSWRPVGNNPSVQQNLNRQQQMRDRGFTRTQNFQQSHTGGGGGGFSR
                     PSGGGGFSRPSGGGGGGGSRPSGGGGGGGRRH"
     gene            complement(2713..>3729)
                     /locus_tag="VKQ52_17200"
     CDS             complement(2713..>3729)
                     /locus_tag="VKQ52_17200"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HLZ88997.1"
                     /translation="KLDPKAKKTKTGQYATGEDVLLKLASRHQIVDDILAYRELTKLK
                     STYVDALPQMINRKTGRVHTSYAQAVAVTGRLSSNNPNLQNIPIRTERGREIRKAFIP
                     RNDDYVLLSADYSQIELRIVAAISGDVNMCAAFRDGKDIHTATAAKVFGVEESAVTKE
                     MRYKAKSVNFGIIYGQGAFGLADNLGISRTEAKEIIDNYKAQFPGIQKYMDDTINFAR
                     ETGYVQTLMGRKRWLKDINSANFTVRGFAERNAINSPIQGSAADMIKLAMIRVHEEFT
                     RQGLTSRMLLQVHDELVFDAYKAEIDIIKPIIMRCMQTALPLPQEVPAIAEMGSGINW
                     LEAH"
BASE COUNT          843 a         1107 c          959 g          820 t
ORIGIN      
        1 gggataggcc gaacggatat attccgccat aacaccgata tgctgcggct tcccgggcag
       61 gatcatcccc ccggaaagca cctcaaaggc caggacggaa cgatattctt catagatgcg
      121 ccggatcacc gggctaaacc cataacacca gccgcaataa gcgtcgtaac aataataaac
      181 tgtcataaaa atgccgggag atcatttccc ccggcaaaaa taaattatcg tgtgttaaat
      241 cacttctaat gtctccggcc gccaccgccc ccaccaccgg aaggccgcga accgccaccg
      301 ccaccgccgc ccgaaggccg gctgaagcca ccaccaccgc tgggtctgct aaaaccgccg
      361 ccaccaccac cggtgtggga ctgctggaaa ttctgcgtcc gggtgaaccc acggtcacgc
      421 atctgctgct gccggttcaa attctgctgc acggacggat tgttccccac cggccgccag
      481 gagttattat tactccgctg ctgccattga ttcgtggcct ggttgcgctg atatacattg
      541 ccgctgcgat cggaaaagac attgttcgct ccgggccttg caaaaccccc gccgttagct
      601 ccaccgggcc ttccaccctg cgggccaacc ggccggccgg cgccgttgaa gccgggccgc
      661 cccgcattgc cgaaagaagc gcctctgcca aaggacgtac ggttgtagaa cgaatgattg
      721 tcccgggtga tcaccccacc gcgaccggca taaatattcg tatggtaatt attgacgtag
      781 acattacggt tacgatagta gtttgcgcca tagaagccat agctccggta cctgttccag
      841 gcataccccg gacgatagta gtggggaccc caccaaccgc cgccggccca gccgccccac
      901 cagccgccat ggaaaccaag accgtagtgg aaccagccaa acccaaatcc atagccaagc
      961 gtccacccgg cccagggatt gtaacagaca ttgaaacccc atgtccaggg ccgcggatag
     1021 tagaacccgt tgaaccaggg atcataatag aaaccggttc catagacgac cgtaggcccg
     1081 taaatatagg tattcagata acccggggta taacccatat acacatagtc cggggttaca
     1141 tcatagatat acacatactt cgtgttatac atgggagaag taggcgggat ccgatccact
     1201 tcatcaggac gctccgttgc taccgtccag ggaccattcg gactgggcga ctggaaccat
     1261 accccattgt ccaccgtata gtagatgcca tgatcgagca taacggaaga agaggtattg
     1321 gtggcatatt gcatgtccgt accctgcaac ggcgagaact ggggttcgcc attgtaattg
     1381 accgaggtat tcgccgaatt gcgatccacc ttggccgtct gcggaacctg cgcatccatg
     1441 atcgcctcac gggcggcatc cgtaccggct acactggcca gcacattgtc tttcggactg
     1501 ccctcgggta tcttggcgaa atcgcccggc aacgaattgc tcgcgacata atgccatcca
     1561 cccgtaaggg accccgacga ataccagcga ccggagatca gcacaaaata cttgcccgaa
     1621 ccctggtcct ggaaaatatc attggtagaa ttgctggcgt aggaaagacc cgtcccggga
     1681 atcgccgtga aactgggctt cccgtcggtc tggatcagct ccgccggcga agtgctcacg
     1741 atgatgtcgg agaccttgtt atcctgcgcg gcgccggccg agtcgatata cccggcattg
     1801 ctgctgttgg cattgtcgac ggcgctcctc acctggtcga gctcggacgg aacattgccg
     1861 gcaggactgt atggaccggt agccgactgc gcctggtacc agtgcttgcc accatacagg
     1921 tagaagccac cgttgttggc ctttacgatc gtgaacggcg tgttgaccac gacatccagg
     1981 ttccagtcct tgttgttctg caacttgggc tgaccatcga tcatgaccag cagcgcggga
     2041 tgatcactgt agatgatcct cggcgcagac gtattcaggt ccttcgataa tttcttctgc
     2101 tccgtattct cgtcaagcga cgaaagcagc tcgtccagcg aaagatcgcc ggccgcctgc
     2161 ggcaggttcg tttccagcgt cgtcttcaga ctgttggtaa agttgtcatc ggtctgaccg
     2221 ggaaacttca cattgggaac cttcacggac tgaatgatca cccggcgatt gtcccggtct
     2281 gtttccacgt tggcaaccga ccagaaagta ccgaacacag gatcggtctt gccgggttgc
     2341 agaacggaga tcgcccagcg ggatttgagg atattatccg caaaagactc cggctggggt
     2401 tcatagatct tgataacggt accgtcatcc gtcgtgaaac tcctgggcca ttcctgttgc
     2461 tgctgctgtt gctgttgggg tggggggcca tcctgggcaa gggccgtacc cataagaaga
     2521 acggcggcaa gcagcataga gaatatagta cgaatgcttc tcatatgtta ttgtttatat
     2581 taattgtaac gaggtgttgg tatatagact cccgtgagac ctcaaagttt gttaccattt
     2641 acaaataaga tgttaaatag ctgcgcgctc gttccgccat ccgcccgcct ccacccgggt
     2701 tttaaccggc ttttaatgtg cttcaagcca attgatcccg gagcccatct cggcaatcgc
     2761 cggcacctcc tggggcaacg gaagcgcggt ctgcatacaa cgcattatta tgggtttaat
     2821 tatatcaatt tcagctttat aggcgtcgaa aaccaattcg tcatgcacct gtagcagcat
     2881 ccgcgaagta agcccctggc gggtaaactc ttcatgaaca cggatcatgg ccagcttgat
     2941 catatccgcg gccgaaccct ggatcgggga gttgatggcg ttccgctccg caaaaccacg
     3001 aacggtgaaa ttggcggaat tgatgtcttt cagccatctc ttacgcccca tcagggtttg
     3061 cacatagcct gtctcccgcg caaaattgat cgtatcatcc atatacttct ggatcccggg
     3121 gaactgcgcc ttgtaattgt cgatgatctc ctttgcctcc gtccggctga tacccaggtt
     3181 atccgcaagc ccaaaggcgc cctggccata gatgatcccg aaattgacac tcttggcctt
     3241 gtaccgcatt tccttcgtca ccgccgattc ctccactcca aagaccttcg ccgccgtggc
     3301 cgtatggata tccttgccat cccggaacgc cgcgcacata ttgacgtcac cactgatagc
     3361 cgcgacgatc cgcagctcga tctgcgaata gtcggccgac aacagcacat aatcgtcatt
     3421 gcgggggata aaggccttcc ggatctcgcg gccccgctcg gtacggatgg gaatattctg
     3481 caggttggga ttgttgctgc tgagccggcc cgtcaccgca acggcctgcg cataactcgt
     3541 atgcacccgc ccggtcttcc ggttgatcat ctgcggcaag gcatccacat aggtcgactt
     3601 taatttggta agctcccggt aagcaagaat atcatcgacg atctggtggc gactggccag
     3661 cttcaacaat acatcttccc ccgtcgcata ctgacccgtc ttcgtttttt tcgccttggg
     3721 gtcgagctt
//
LOCUS       DATGFG010000909         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1534859, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000909 DATGFG010000000
VERSION     DATGFG010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534859"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VKQ52_17205"
     CDS             <1..1272
                     /locus_tag="VKQ52_17205"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLZ88998.1"
                     /translation="YSQWGVVESWSICPEDEGWEQRKGPYSANYFDYVQAYEKLQTTF
                     NPTRFDPAKWATAAKEAGMKYVVFTTKHHDGFSMFDTKQTDYKITSTQTPFHTNPKSN
                     VAKEVFDAFRTDGFMIGAYFSKPDWHSNDYWWRYFPPKDRNVTYDPARYPDRWKAYKD
                     FTYNQIQELMTGYGRMDLLWLDGGWVRPLYTVDPAVEWQRGIRYDQDIDMPKIAAMAR
                     SHQPGLIVVDRSVQGEYENYTTPEQQVPVHPMDDPWETCMTMGDSWSYVPHDHYKSTT
                     QLVQLLVKIVSRGGNFLLNVGPSPEGDWDDTAYVRLKEMGAWLKVNGEGIYNSRTIAP
                     WSSGNVYLTQSKDSLNVYAFFLGEGKDVVLPAEVSIGKFTARPKSRVTILGRKEKLKW
                     RQEGKKMVIGIPAALQNKPVGEYAVTFRISS"
     gene            complement(1262..>2719)
                     /locus_tag="VKQ52_17210"
     CDS             complement(1262..>2719)
                     /locus_tag="VKQ52_17210"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ88999.1"
                     /translation="LSGAAGVLDYTHHEDPQRDFNRQHLLPHYYSHNGPCMATADING
                     DGLEDIFIGGSKGNPGALFVQTKDHRFVRSPSTELLADSLSEDVDAVFFDADGDGHPD
                     LYVVSGGYAYAEHSPDLQDRLYINDGKGHFTRKRDALPANTGNKSCVRVCDLNNDGKP
                     DLFVGGGVAPGGYPRSCASSIYLNDGHGRFRDATDEWNPQLKQLGIVTDAAWVDVNGD
                     KVKDLVVVGEWMPVKVFVDQNGRLTDRSSEMVPFASNGWWNKIAVADLDGDGIDDLVL
                     GNYGLNSPLHASPQEPVELYTTDIDKNGIPDPIMTCYRDHVSYPFFPMDDILQQVPSL
                     KKKFYNYEVYANATIKDIIPEEGARSLRPLTANTFQTLWLRNTGKGFERKELPIQAQY
                     SPVYSIAAVDLDHDGHKDLVLAGNNIYNKILLGRDDANHGLVLLNDGKGNFRYLPPAR
                     SGLTIRGDNRSIETIGDEMFFGMNDQPVRVFRLRN"
BASE COUNT          601 a          783 c          775 g          560 t
ORIGIN      
        1 tatagccagt ggggggtcgt ggaaagctgg agcatctgcc ccgaagacga gggttgggaa
       61 cagagaaagg gaccctattc ggccaactac ttcgattatg ttcaggccta tgagaaattg
      121 cagacgacct tcaaccccac gcgcttcgac ccggcaaaat gggccacggc ggcaaaggag
      181 gcggggatga aatatgtcgt cttcaccacc aagcatcacg acgggttctc gatgttcgac
      241 acgaaacaga ccgattacaa gatcacctcc acccagactc ccttccatac caatccgaag
      301 agcaatgtcg caaaagaagt gttcgacgct ttccggacgg atggattcat gatcggcgcc
      361 tatttctcca agcctgactg gcatagcaac gactactggt ggcgctattt cccgccaaag
      421 gatcgcaacg tcacgtatga cccggccaga tatcccgacc gctggaaggc ctataaggat
      481 tttacctata accagatcca ggagctgatg acgggctatg gccggatgga cctgctctgg
      541 ctggatggcg gctgggtgcg gcccctctac accgtcgacc cggccgtgga atggcaacgg
      601 ggcatccgct atgaccagga tatcgacatg ccaaagatcg ccgcgatggc gcgtagccat
      661 caaccggggc tgatcgtcgt cgaccgcagc gttcaggggg aatacgagaa ctataccacg
      721 cccgaacaac aggttcccgt ccatccgatg gatgacccct gggaaacctg tatgacgatg
      781 ggagactcct ggagctatgt cccccacgat cattataaat ccacgaccca gttggtgcag
      841 ttgctggtaa agatcgtctc ccgcggtggc aatttcctgt tgaatgtcgg accatcaccg
      901 gaaggcgact gggatgatac ggcctatgtc cgtctgaagg aaatgggcgc ctggctaaag
      961 gtcaacgggg aaggcatcta caacagcagg accatcgccc cctggtcttc cggcaatgta
     1021 tacctgaccc aatccaaaga cagcctcaac gtatacgcct tcttcctggg ggaaggaaag
     1081 gatgtcgtac tgcccgcgga ggttagcatc gggaaattca cggccaggcc caagagccgg
     1141 gttacgatcc tgggccggaa agagaagctg aaatggcggc aggaggggaa gaagatggtg
     1201 atcgggatcc ccgcggcctt acagaataaa ccggtgggtg aatacgcggt gaccttccgg
     1261 atcagttcct gagccggaat accctgacgg gttgatcgtt catgccgaag aacatttcat
     1321 cgccgatggt ctcgatgctg cggttatccc ccctgatggt tagccccgat cgcgcgggcg
     1381 gcagatagcg gaaattgcct ttgccgtcat tcagcagcac caaaccgtgg ttcgcatcgt
     1441 ccctgcccag gagtatctta ttgtagatat tattcccggc aagcaccagg tccttatgcc
     1501 cgtcatgatc gaggtcgacc gccgcgatcg aatagacggg cgaatactgg gcctgtatgg
     1561 gcagctcttt ccgctcgaag cccttgcccg tgttccgcag ccagagggtc tggaaggtat
     1621 tcgccgtcag ggggcgcagc gaccgcgccc cttcctccgg aataatatcc ttgatggtgg
     1681 cattggcgta gacctcgtaa ttatagaatt ttttcttcaa cgaaggcacc tgctgaagga
     1741 tatcatccat cgggaagaaa ggataggata catgatcacg gtaacaggtc attatcgggt
     1801 cggggatacc gtttttgtcg atgtccgtgg tgtacaattc cacgggttcc tgcggcgaag
     1861 cgtgcagggg ggaattcagg ccatagttgc ccagtacgag gtcgtcaatc ccgtcgccat
     1921 cgagatcggc cactgctatc ttattccacc agccattact ggcaaaagga accatttcgc
     1981 tgctgcgatc ggttagccgc ccgttttgat cgacgaatac tttcaccggc atccactcgc
     2041 ccaccacgac aaggtccttc accttgtccc cgttcacgtc cacccaggcg gcatcggtca
     2101 cgatgcccag ctgcttcagc tgggggttcc actcgtccgt cgcatccctg aaccgcccat
     2161 gcccgtcgtt gagatagatg ctgctggcgc agctgcgcgg atagcccccg ggcgcgaccc
     2221 cgccacctac aaacaggtcg ggcttgccgt cattgttcag gtcgcaaacg cgcacacaac
     2281 tcttgttgcc cgtgttcgcc ggcagcgcat cccgtttccg ggtgaaatgg cctttgccat
     2341 cgttgatata gaggcggtcc tgcaggtccg gggaatgttc cgcatatgca tatcccccgc
     2401 tgacgacgta aagatcggga tgaccgtccc cgtcggcgtc aaagaaaacg gcgtccacat
     2461 cttcgctcag cgagtcggcc aataattccg tcgacggaga gcggacgaac cggtgatctt
     2521 ttgtctgaac gaaaagggcg ccgggattac ctttcgatcc cccgatgaag atatcctcca
     2581 gcccgtcgcc gttgatatcc gccgttgcca tgcagggccc gttgtgcgag taatagtgag
     2641 gcaggagatg ctgccggttg aaatcgcgct gcgggtcctc gtgatgcgta tagtcgagca
     2701 ctccggcggc gccggagag
//
LOCUS       DATGFG010000910         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_640676, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000910 DATGFG010000000
VERSION     DATGFG010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640676"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2020)
                     /locus_tag="VKQ52_17215"
     CDS             complement(<1..>2020)
                     /locus_tag="VKQ52_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89000.1"
                     /translation="TTDGGKTWRRVLFVNDQTACSDVVMEPGNPSVLYAGMWHVRRTP
                     YSMESGGEGSGLYKSTDGGETWVNLSAKKGLPKGTWGVVNIAVAASNPDKVYAMIENA
                     QGGLFVSTDRGDTWTKTNSENDIRQRAWYYNRVYVDPTNENTVYCLNVEFMRSIDGGK
                     TFTHVPSPHSDHHDLWIDPTDGHRLILADDGGAQVSYNAGGDWSAVDNQPTAQIYRVS
                     TDNAFPYHILGAQQDNSSVRIKSRTFGPAITERDWESTAGFESGYIVADPLNPDIVYG
                     GNYDGFLGRLNHRTGENRAIDVWPDNPTGAGADSLKFRFQWNYPIFFSPHNPHRLYAA
                     GNELFMTDNEGQSWTAISPDLTTNDKSKQGPSGGPITKDNTSAEYYCTIFTATESPLE
                     RGLLYTGSDDGLVHVSRDSGAHWEPITPPAAGKWTLWNCIETDPFKKGTAWVVGTRFR
                     LDDYTPYIFRTEDYGKTWKLATKGIDPQHFARVVRADHRRPGLLYAGTEYGVYISYDG
                     GDNWSPFQLNLPIVPVTDLTLKDNDLIVATQGRAFWALDDLSLLQQHGPDALQQRLHV
                     FSVNDAWRTEGGGGRRNRETVTRNAGKNPPNGVVFYYWLKDAPDSPNVSITILDKQHQ
                     PIRTFSTKSKDADTRLECNTGMNSFVWDMLYAPAEKIPGMLLWTGGAGS"
BASE COUNT          366 a          596 c          616 g          442 t
ORIGIN      
        1 gctgccggcc ccgccggtcc acagcaacat accggggatc ttctccgccg gcgcatagag
       61 catatcccag acaaagctgt tcattcccgt attgcattcc agccgcgtat cggcgtcctt
      121 cgacttcgtg ctgaaggtac ggatgggctg gtgttgtttg tccaatatcg tgatcgagac
      181 gttcggggaa tccggggcgt ccttcagcca gtaatagaag accaccccgt ttggcgggtt
      241 cttgccggca ttcctggtca ccgtctcccg gtttctcctt cccccacctc cttcggtgcg
      301 ccaggcatcg ttgacgctaa agacatgcag ccgctgctgc agggcatcgg ggccatgctg
      361 ctgcagcagg ctcaggtcat ccagggccca gaaagcgcgg ccctgggtgg ctacgataag
      421 atcgttgtcc ttgagggtga gatcggtcac cggtacgatg ggaaggttca gctgaaaggg
      481 ggaccagttg tcgccgccgt catagctgat atacaccccg tattccgtgc cggcatagag
      541 cagaccgggc cggcggtggt cggcgcggac gacacgggca aaatgctgcg ggtcgatgcc
      601 cttcgtcgcg agcttccagg tcttgccgta gtcctcggtg cggaagatat agggggtgta
      661 gtcgtcgagc cggaaacggg tacccacgac ccaggccgtc ccttttttaa agggatcggt
      721 ctcgatgcag ttccacagcg tccatttccc tgcggcgggt ggcgttatcg gctcccagtg
      781 cgcaccgctg tcgcggctca catggacaag cccgtcatcg ctgccggtgt agagcaggcc
      841 ccgctccagc ggcgactccg tcgcggtaaa gatcgtacaa tagtattcgg ccgaggtatt
      901 gtccttggtg atggggccgc cgctgggtcc ctgtttcgat ttatcgttcg tcgtcagatc
      961 gggactaatc gccgtccagc tctggccttc gttatcggtc atgaacaatt cgttgcctgc
     1021 cgcatacaac cggtgcgggt tatgcgggga aaagaaaatg gggtaattcc actggaagcg
     1081 gaatttcagg gagtcggcgc cggcgcccgt agggttgtcc ggccagacgt cgatggcgcg
     1141 gttctcgccg gtacgatggt tgaggcggcc gagaaaaccg tcgtaattgc cgccatacac
     1201 gatgtcggga ttcagcggat cggcaacgat atagccgctt tcaaaacctg ccgtcgactc
     1261 ccagtcgcgc tcggtgatgg ccggcccaaa ggtccggctc ttgatgcgca cgctggaatt
     1321 gtcctgctgg gcgccaagaa tatgataggg gaaggcattg tcggtcgaga cgcgatagat
     1381 ctgggccgtg ggctggttat ccaccgcact ccagtccccg ccggcattgt agcttacctg
     1441 tgcgcccccg tcatcggcca ggatgagccg gtggccgtcg gtgggatcga tccagagatc
     1501 atgatggtcg ctatggggtg aaggaacatg ggtaaaggtc ttgccgccat cgatgctgcg
     1561 cataaactcg acgttcaggc aatagacggt gttctcgttc gtggggtcga catataccct
     1621 gttgtaatac caggcgcgct gccggatatc gttctcgcta ttggtcttcg tccaggtgtc
     1681 gcccctgtcg gtgctgacga agaggccgcc ctgcgcattc tcgatcatcg catatacctt
     1741 atcgggattg gaagcggcaa ccgcgatatt gaccacaccc catgttccct tcggcaaacc
     1801 ttttttcgcc gacaggttga cccatgtctc accgccatcg gtgcttttgt acaggccgct
     1861 gccttctccc ccgctctcca tactgtaggg cgtacgccgg acatgccaca tgccggcata
     1921 caacaccgaa ggattgccgg gctccatgac gacatcggaa caggccgtct gatcgttgac
     1981 aaagagtacg cggcgccagg tcttgccgcc atccgtggtc
//
LOCUS       DATGFG010000911        11237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1097904, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000911 DATGFG010000000
VERSION     DATGFG010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097904"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VKQ52_17220"
     CDS             complement(<1..867)
                     /locus_tag="VKQ52_17220"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ89001.1"
                     /translation="MIRAALKRPITVLVFFIGLLLFSVMAVRSIPIDIFPRLNLPTIY
                     VIEQYGGMSAQQMEGFFATRMQDQFLYVNGIKTIESRNIQGLSLIRLTFYENTDMAEA
                     SAQVALQVNRTMAFFPPGALPPQVVRFDASSLPVGELVFSSKTRPLKEMFDLAITRVR
                     PLFATVPGLSAPPPFGSNARSVLINVSPEKLRMYNLSADEVVAALTKNNAITPSGNIR
                     LDSMMLVSSINSLEGKVQHFEDIPVHSNGSSVIYVRDVATVADGADITVDYALVNGKR
                     SVYIPVVKTADAS"
     gene            complement(890..1972)
                     /locus_tag="VKQ52_17225"
     CDS             complement(890..1972)
                     /locus_tag="VKQ52_17225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ89002.1"
                     /translation="MNKNIFLFLLSTSLFICGCSNDKAAEKKTAAPTEPHYAMATAEK
                     QSVEQVYKLPAQLAAYQEVSIFPKVNGYVKTVLVDIGSHVGAGQLLMVLEDPELEQAA
                     AQAREKYYQTVSSYTISRENYERLKQAAQTPGAISPMDLATARSKTEADSALSNAEKA
                     AWQQQQVMLEYLRVTAPFKGVITQRNVHPGALVSAEAKDGKPMLELKEVDHLRLQVSI
                     PESMAGSLRDNDTLSFYLSSYPGKRFTGHIARKAMNINQQYRSEPVELDVYNADEKLS
                     PGMYADVLFDSKGNPNALSVPKTAVVTSTERKYVIAVRDGRTVKVDVSTGNEGSSRIE
                     VLGDLKPGDQVVADANDEIKEGITLK"
     gene            complement(1984..5229)
                     /locus_tag="VKQ52_17230"
     CDS             complement(1984..5229)
                     /locus_tag="VKQ52_17230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ89003.1"
                     /translation="MVRTALKRPITIIVLFTGLLLFSVLAIRSIPIDIFPKLNSPTIY
                     VIEQYGGMSAQQMEGFFATRMQDQFLYVNGIKNIESRNIQGLSLIKLTFYENTDMAEA
                     SAQVALQVNRTMAFFPPGALPPQVVRFDASSLPVGELVFSSKTRPLKDIFDLAITRVR
                     PLFATVPGLSAPPPFGSNARSVLINVNPEKLRMYNLSADEVVQAISRNNAITPSGNLR
                     VDSIMYVTSINSLESKVQHFEDIPVRSNGNSIIYVHDVATVADGADITVDYALVNSKR
                     SVYIPVVKTADASTWEVVQTLRKRLPEMKSLLPDDVDISYEFDQSVFVINSAKSLMTE
                     GILGAVLTGLMVLLFLRDWRSSLVVIITIPVAVMGAVFFLKLADQTINIMTLSGLALA
                     IGILVDQATVTIENIHQHLEMGSSKKQAILDACSEISFPLLLILLCILAVFAPAFVMS
                     GVPKAMFLPLSLSIAFAMIVSFVAAQTLVPVISVWLLKAEKFQYHHQGAHAHAGLALN
                     PGERSEVAMHGRQEQEHKEKNDFFQRIKSGLASRLEKWMPRRRLVVTLYLLFAFGAAG
                     FCFTIIGKDLLPKTNTGQLQLRVREPDGTRLEVTERVTQGVLDIIDSTVHHRIAISSA
                     YVGLVPSNFGTSNLYIFNSGTHESVIQVNLDENYSVKIEDLKETLRKNILAAYPQVKL
                     SFEPIELTEKIMAQGASTPIEVRVAGKNMNDIQSYAGKLVDTLKEIPFLRDVQIAQPL
                     RLPTVEINVDRNKLALMGLTIDNVSRSITDVTSSSRYTNKNLWLDERAAYTYQSQVQV
                     PEYIMNSMQQLESVSLVPGQNRPVLSDVASFRIDTLPGEYDRSGPRRYVTVSANIYRK
                     DLGSATAAVQKAIDKLGAPPTGLVPEIKGMSSLLTETLNSLQTGLLAAIVVILLLLAA
                     NYQSFGVSVSVLATVPAVLLGSMLLLLATGATLNLQSYMGIIMSTGVSVANAILIVTN
                     AEKLRLEYRDPFKAAIAAASIRLRPILMTSLAMIAGMIPMASGLGEAGDQSAPLGRAV
                     IGGLAASTFAALFIVPLVYGWIQQNASFDTVSLLPEDSPEPPSTPGTDIKY"
     gene            complement(5240..6637)
                     /locus_tag="VKQ52_17235"
     CDS             complement(5240..6637)
                     /locus_tag="VKQ52_17235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ89004.1"
                     /translation="MIAFCTGKFRRAIGLFLLLPSLSVFAQKKYSLKDFIDSAQRHLP
                     VLLQKQALVEAAKAGVTDARHAFLPTSYIGDEMTVGTDNSIPGSYISFGIIPSSSSGV
                     RSSNIYQSAIGNIGFFYNQYELLDFGLKKSTVRNAQAYLQLTQADRDREAYLIKWQVA
                     RLYLDILKSEFQLAIDQENANRYDTVFRVIQAVTESGIKPGADSSLAKAELSKARITY
                     NQTFGEIRQLQQQLAYLSGISVNEVAIDTSNTHNYLSMLSVIASPPSGGPVNPLIDFF
                     DKERDLYLQREDLVKKTYLPRVLLNGVGWARGSSIDYTNNYKSSAVSGLGYQRFNYLA
                     GLTLEYDLFNIVHRRDKERIARNNTLASEYGLQQEQLSLQNVGNKADEAIRTATKNLV
                     EIPVQIGAAEDAFNQKTAQYKAGIINLVDLTNASFVLYRSQSDYVQTLSDWLLANLDR
                     YSATGSLDQFIQSIK"
     gene            complement(6686..7066)
                     /locus_tag="VKQ52_17240"
     CDS             complement(6686..7066)
                     /locus_tag="VKQ52_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89005.1"
                     /translation="MRKNLHKISFYTLILAVYGIFFSIQSFYNFEGHSDAREILRHAA
                     FLPVVSSGSGVKAIPAAHPDSHRVRLRLNKRFHQEEFPPCTVYEVCVPVIYVTPHALG
                     PFAIRPLPAVTLFHPQLRGPPYIA"
     gene            complement(7152..8168)
                     /locus_tag="VKQ52_17245"
     CDS             complement(7152..8168)
                     /locus_tag="VKQ52_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HLZ89006.1"
                     /translation="MILLLTIIVLALIFDYINGFHDAAISIATIVSTKVLTPFQAVLW
                     AAIFNFGAFFIFNNYFGEFKIGNTIAHFVNRDFITLPVILSGLIAAICWNLFTWWFGI
                     PSSSSHTLLGGFVGAALAHAGGWTAHGKQVIDYGSVIPTFFFIILAPLIGFLVALVIT
                     ILIVNLFQRSNPYKAESWFRRLQLLSSALLSIGHGGNDAQKVLGIIAAAMIAHGDLGN
                     IKDIPHWVPIACFSAIALGTMSGGWRIVKTMGTRITKVNPLEGVCAETAGAATLFLTQ
                     YLGIPVSTTHTITGAIIGVGATKRMSAVRWGVTVNLIWAWILTIPVSALLAAGIYWVV
                     RFFL"
     gene            complement(8231..8881)
                     /locus_tag="VKQ52_17250"
     CDS             complement(8231..8881)
                     /locus_tag="VKQ52_17250"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HLZ89007.1"
                     /translation="MKGFNAFVQLFMPKDKVFYSLFEEVADGVARMGELLKDVVSEPD
                     FDKRAAIISQLEAQEHVNDDLTHRIFTELGRNFITPFDREDIHYLASALDDICDYIYA
                     AAKKINFYKVNPNDQGIQKMADLIEQGARQIRGAVRELRNMRDMRKITDALVRVNSIE
                     NQADDIFDMSIERLFETEPDAKEVIKKRDIYQVMEIVTDKCEDATNVIESIIIKYA"
     gene            9224..10555
                     /locus_tag="VKQ52_17255"
     CDS             9224..10555
                     /locus_tag="VKQ52_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89008.1"
                     /translation="MKRNFAKQFLFIVAFFLLTIASQAQVFVNNDSAFSAGTPKAGRL
                     WGYAFGDFYYKSHSDSLNRGGANQYTGIPQSRNAFAFRRIYLGYDYNFNKKFSSELLL
                     SAEDNFPAGNPPSTVASGSSGDLLANGKESFFIKLMNVRMKNIWKGTDLVLGEQFTPS
                     FAMLTEKVWNYRSIERTISDIRRTPSYDLGVGLNGTFDPATKNFGYNLLMATGNSDKP
                     ATNSFKWWYGDVWGMFLDKHLILDVYADYQRLNWQPGWHHDRQMLKAFAAWSSQPITV
                     GVEGFVNNMAVDTKASKIGGGADTIATMANGIAMFVHGDIITNKLRFFARVDLYNPNS
                     KVDNSKYNAYSALGSPGGYMTPGYKMNFSPSTGSPVTATSTGDPTAKETFITAGLDFM
                     PFKDIHLEPNLWYEHYASQLATGTKADNDVVFRLTFFFVFGKNYKNSYTQL"
     gene            10582..>11237
                     /gene="pstS"
                     /locus_tag="VKQ52_17260"
     CDS             10582..>11237
                     /gene="pstS"
                     /locus_tag="VKQ52_17260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HLZ89009.1"
                     /translation="MKKTMPIFRGTLTVAVLVFGSLFASMSLVSCGGGGGSASGSDSA
                     ASAGSSDNMLTGAGSSFDNPLFSKQFSEYDKANNQKINYQSVGSGAGISQLTAKTVEF
                     GASDAPMNGKQDSALSAPVVHVPITEGAVVLSYNLPEVKDTLKLVPGVIADIFLGKIT
                     KWNDPKIAADNKGVKLPATAITVAHRSDGSGTSNIFTTYLSKVSEDWSKTVGKGSSVN
                     "
BASE COUNT         2452 a         3022 c         3227 g         2536 t
ORIGIN      
        1 ggaggcatcg gccgttttca ccacggggat atagaccgat cgcttcccgt tcaccagcgc
       61 atagtccacg gtgatatcgg cgccgtcggc cacggttgcc acatcgcgta catagatgac
      121 actgctgccg ttgctgtgga cggggatatc ctcgaaatgc tgcacctttc cttccaggga
      181 attgatggaa ctcaccagca tcatgctgtc cagccggata tttccggaag gggtgatggc
      241 attgttcttt gtcagcgcag ccacgacctc gtccgcggag agattgtaca tccggagctt
      301 ctcggggctg acattgatga gtaccgaccg ggcgttggaa ccgaatggcg gcggcgccga
      361 gagccccggg acggtggcga agagcggccg cacgcgggtg atggccaggt cgaacatctc
      421 tttcagcggc cgggtcttgc tgctgaagac cagttctccc accggcaggg aggaggcgtc
      481 gaaacgcacc acctgcgggg gtaacgcacc gggcggaaag aaggccatgg tccggttcac
      541 ctgtaaggct acctgtgccg aggcctcggc catgtcggtg ttctcataga aggtgagcct
      601 gatcagggaa agtccctgga tattgcggct ttcgatggtc ttgatgccgt tgacatagag
      661 aaactggtcc tgcatgcggg tagcgaagaa accttccatc tgctgcgccg acatcccccc
      721 atattgctcg atcacataga tcgtcggcag gttcagccgg ggaaagatat cgataggaat
      781 gctccgcacc gccatgaccg agaacagcaa taacccgatg aagaacacca ggacggtgat
      841 aggccttttt aaggctgctc ttatcatact atttttttta aggtcatggt tatttcaggg
      901 tgatcccctc ttttatctcg tcgttggcat cggcgaccac ctggtcgccg ggtttcagat
      961 cacccagcac ttcgatccgg ctgctgcctt catttcccgt cgaaacatcc acctttacgg
     1021 tcctgccgtc gcggacagcg atgacatatt tgcgttcggt agaggtcacc acggccgtct
     1081 tgggtaccga caaggcgttg ggattgccct tgctgtcgaa aagcacgtcg gcgtacattc
     1141 ccggcgacag tttctcgtcg gcgttgtata cgtccagctc gaccggctcc gaccggtact
     1201 gctggttgat gttcattgct ttccgggcga tatgtcccgt gaaccgtttc ccggggtaag
     1261 agctcaggta aaaagagagg gtatcgttgt cgcgaaggga gccggccatg ctttcgggga
     1321 tggatacctg tagccggaga tgatccactt ccttcaactc gagcatcggc ttcccgtcct
     1381 ttgcctcggc gctgacaagg gcgccggggt ggacgttgcg ttgggtgatg acccccttga
     1441 acggcgccgt cacgcgcagg tattcgagca tcacctgttg ttgctgccag gcggcctttt
     1501 ccgcgttgct cagcgcactg tccgcctccg tcttcgatct tgccgtagcg aggtccatgg
     1561 gtgatatggc tcccggtgtt tgggccgcct gtttcagccg ctcgtaattc tcccggctga
     1621 tggtatagga ggatacggtc tggtaatact tttcccttgc ctgcgctgcg gcctgctcca
     1681 gttccgggtc ttccagcacc atcaggagct ggccggcgcc cacatgcgag ccgatgtcca
     1741 ccagtacggt cttcacataa ccgtttacct tggggaagat gcttacttcc tggtaggcgg
     1801 ccagctgggc gggcagctta tacacctgtt cgacgctttg tttttcggcc gtggccatcg
     1861 cataatgggg ttccgtgggc gccgcggtct tcttctccgc agccttgtcg ttcgagcagc
     1921 cgcagatgaa cagcgaggtt gataagagaa ataaaaaaat attcttgttc atatagtttg
     1981 ttgttaatat ttaatgtctg tcccgggggt cgaaggtggc tccggggagt cttcgggtaa
     2041 aagagagacc gtgtcgaaag atgcgttttg ctgtatccag ccatacacca gcggtacgat
     2101 gaacagggcc gcgaaggtgg aggcggccag cccgccgatg acggcgcggc caaggggcgc
     2161 cgactggtca ccggcttcgc ccaatccact cgccatgggg atcatcccgg cgatcatcgc
     2221 cagcgaggtc atcaggatcg gccggaggcg gatacttgcc gccgcgatgg ccgctttgaa
     2281 ggggtcacga tattccagcc gcagcttttc cgcattggtg acgatcagga tcgcattggc
     2341 caccgacacc cccgtcgaca tgatgatacc catatacgac tggaggttga gtgtggcgcc
     2401 cgttgccagc agcagcagca tcgaacccag cagtaccgcg ggcaccgtcg ccagcaccga
     2461 gaccgaaacc ccaaaggatt ggtagttggc tgccagcaac aggaggatga cgacgatggc
     2521 ggccagcaga cccgtttgca gggagttaag cgtttccgtc agaagggagg acatcccttt
     2581 tatctccggt acgagacccg taggcggggc gcccagctta tcgatagctt tttggacggc
     2641 tgccgtggca gagccgaggt ccttcctgta gatatttgcg ctgacggtaa catagcggcg
     2701 cgggccggag cggtcatatt ccccggggag cgtatcgatc cggaaactgg cgacatccga
     2761 gagcaccggc ctgttctgcc ccggcaccag cgacaccgac tccagctgtt gcatcgaatt
     2821 catgatatac tccggcacct gcacctggct ttgataggtg taggccgccc gctcgtccag
     2881 ccaaaggttc ttgttggtat accggctgga agaggtgaca tccgtgatgc tcctggagac
     2941 gttgtcgatg gtgagtccca tcagcgccag cttattccgg tcgacattga tctcgacggt
     3001 gggcaggcgc agcggctgcg cgatctggac atcccgcaga aagggaattt ctttcagggt
     3061 atcgaccagc ttaccggcgt aggattgaat gtcgttcatg ttcttgccgg ccacccgcac
     3121 ctcgatgggg gtggaggcgc cctgcgccat gatcttttcc gtgagttcga taggctcgaa
     3181 ggaaagtttc acctgcggat aggccgcgag gatattcttt cgcaatgtct ctttgagatc
     3241 ttcgatcttc acggaataat tttcgtcgag gttcacctgg atcaccgact catgggttcc
     3301 gctgttgaag atgtacaggt tgctggtgcc gaaattgctg ggcaccagtc cgacataggc
     3361 cgaggaaatg gcgatccggt gatgcacggt gctgtcgatg atatcgagga ctccctgcgt
     3421 cacccgttcg gtcacttcga gccgcgtccc gtcgggttcg cgcacccgca gctgcagctg
     3481 gccggtattg gttttgggca gcaggtcctt gccgataatg gtaaagcaga agccggcggc
     3541 gccaaaagca aaaagcagat agagggtaac gaccagcctg cggcggggca tccatttttc
     3601 caggcgcgag gccaggccgc tttttatccg ctgaaaaaaa tcgttctttt ccttgtgttc
     3661 ctgctcctgc cggccatgca tggcgacctc gctgcgttca cccggattaa gcgcaagacc
     3721 cgcatgagca tgcgctccct ggtggtgata ctggaatttt tccgctttga gcaaccagac
     3781 actgatcacg gggacgaggg tctgcgccgc gacgaaactg acgatcatcg cgaaagcgat
     3841 ggacagggag agcggaagga acatggcttt tggcactccc gacatgacaa aggccggggc
     3901 aaagacggcc aggatacaaa gcaggatcag cagcagcgga aagctgatct ccgaacaggc
     3961 gtccagtatc gcctgtttct tgctgctgcc catctcgagg tgctggtgga tattctcgat
     4021 ggtcacggtc gcctggtcca ccaggatgcc aatggccagc gccagcccgc tcagggtcat
     4081 gatattgatc gtctgatccg ccagcttgag gaagaagacg gcccccatca ccgcgacggg
     4141 aatagtgatg atcaccacca gactgcttcg ccagtcccgt aggaacagca gcaccatcaa
     4201 ccccgtgagc acggctccca ggatgccttc cgtcatcagg ctcttggcgg agttgatgac
     4261 gaacaccgac tggtcgaatt catagctgat gtcgacgtcg tcgggcagca ggctcttcat
     4321 ctcgggaagg cgtttgcgca gggtctgcac cacctcccag gtggaggcat cggctgtttt
     4381 tacgacgggg atatataccg accgcttgga attcaccagt gcatagtcca ccgtaatgtc
     4441 ggcgccgtcc gcgaccgtcg ccacatcgtg cacatagatg atgctgttgc cgttgctgcg
     4501 gacggggata tcctcgaaat gctgcacctt gctttccagc gaattgatcg aggtgacata
     4561 catgatgcta tccacccgga ggttgcccga gggagtgatg gcgttgttcc gggagatagc
     4621 ctgtaccacc tcgtcggccg agaggttgta catccggagc ttttccgggt ttacattgat
     4681 cagcaccgag cgggcattgg agccgaaagg cggcggcgcc gagagtcccg gaacggtagc
     4741 gaagagcggc cgtacgcggg tgatcgccag gtcgaagata tcttttagcg gccgggtctt
     4801 gctgctgaag accagttccc ccaccggcag ggaagaggcg tcgaaacgta ccacctgcgg
     4861 cggcaaggcg ccgggcggga agaaggccat ggtccggttc acctgcaggg ccacctgcgc
     4921 cgaagcctcg gccatatccg tgttctcata gaaggtgagc ttgatcaggg aaagaccctg
     4981 gatattgcgg ctctcgatat tcttgatccc gttgacataa aggaactggt cctgcatccg
     5041 ggtggcgaag aagccttcca tctgctgcgc cgacatccct ccatactgct cgatcacata
     5101 gatcgtgggt gagttcagct tgggaaagat atcgatgggt atcgaccgga tcgccaggac
     5161 cgagaacaac agcaggccgg tgaacaggac aatgatcgta ataggccttt ttaaagctgt
     5221 tcgtaccatt attatactat tatttgatag attgaataaa ttggtccagc gacccggtgg
     5281 cggaatacct gtccaggttg gccagcagcc agtcgctcag ggtctgtaca tagtctgatt
     5341 gcgaccggta gaggacaaag gacgcattcg tgagatcgac caggttgatg atccccgcct
     5401 tgtactgcgc ggttttctga ttgaaggcat cttcggcggc gccgatctgg acagggatct
     5461 cgacaaggtt cttcgttgcc gtccggatcg cttcatcggc cttattgccc acattctgca
     5521 gggacagttg ctcctgctga aggccatatt cgctggcgag cgtattgttc cgcgcgatgc
     5581 gctccttatc ccggcgatgt acgatattga acagatcgta ttccagcgta agtcccgcga
     5641 ggtagttgaa tcgctggtat ccaagcccgc tgaccgccga agacttgtaa ttattggtat
     5701 aatcgatgct ggagcccctg gcccagccga cgccgttgag caacacccgc ggcagatagg
     5761 tctttttgac caggtcttcc cgctgcagat aaagatcccg ttccttatcg aaaaagtcga
     5821 tcagggggtt gacgggcccg ccggagggcg ggctggcgat gacgctcagc atggacagat
     5881 agttgtgtgt attggaagtg tcgatcgcca cctcgttgac ggagatccct gacagatagg
     5941 ccagctgttg ttgcagctgc cggatctcgc cgaacgtctg gttataggtg atccgggcct
     6001 ttgacagctc ggccttggcg agggaggaat ccgctcccgg cttgatcccg ctttcggtca
     6061 ccgcctggat caccctgaaa acggtgtcat agcggttggc gttttcctga tcgatggcca
     6121 attgaaactc gcttttcagg atatccaggt aaagcctggc tacctgccat ttgatcagat
     6181 aggcttcccg gtcgcggtcg gcctgtgtga gctgcagata ggcctgcgcg ttgcgcacgg
     6241 tggatttttt cagcccgaaa tccagcaact cgtactgatt gtaaaagaac ccgatattgc
     6301 cgatggcgga ctggtaaata ttggacgacc ggacaccgct cgaactggag gggatgatgc
     6361 cgaaggaaat ataggacccg gggatcgaat tgtcggttcc caccgtcatt tcatcaccga
     6421 tgtatgaagt gggcaggaag gcgtgccgcg cgtcggtgac gccggctttc gcggcctcga
     6481 ccagggcctg tttttgcagg aggaccggca ggtggcgttg cgcgctgtcg atgaagtcct
     6541 taagggaata ttttttttgt gcaaatacgg aaagggaagg caacagcagg aataagccga
     6601 tagccctacg aaactttccg gtacagaaag cgatcataca aactaggata aattatgaaa
     6661 tgaagggaag tcaggtagtt aacgatcagg cgatgtaggg gggaccccgc agctgcgggt
     6721 ggaaaagggt aacggcgggc aggggacgga tggcaaaggg gccgagggca tgcggcgtta
     6781 cgtaaataac gggtacgcag acctcataga ccgtacaagg gggaaattct tcctggtgga
     6841 accgtttgtt gagccggagc cggacccggt gtgagtcggg atgggcggcg ggtatggctt
     6901 ttactccgga tccggaagat acgacaggga gaaaagcagc atgacgaagg atctcccggg
     6961 catcggaatg tccttcgaaa ttgtaaaagg actggatgga gaagaagatc ccatacacgg
     7021 ccaggatcag cgtgtagaag gagattttat gtagattctt cctcatcgtc ggcgaaatta
     7081 gcgataaaat cccagcttac tctgccgtcc acattaaaga atggttaaaa gaaggccgag
     7141 gtcccggcgg atcacaggaa aaatcgtacc acccaataga taccggcggc caggagggcg
     7201 ctgaccggga tggtcaggat ccaggcccag ataagattga ccgtcacacc ccagcggacc
     7261 gccgacatgc gtttggtcgc cccgaccccg atgatggcgc cggtgatcgt gtgggtggtg
     7321 ctgaccggga tacccaggta ttgggtgagg aacagggtag cggccccggc cgtctccgcg
     7381 cagacccctt ccagcgggtt taccttggtg atccgggtcc ccatggtctt tacgatccgc
     7441 caacctccgc tcatcgtgcc gagggcgatg gccgaaaaac aggcgatcgg cacccagtgc
     7501 gggatatctt ttatattacc caggtcgcca tgggctatca tcgccgcggc gatgatcccc
     7561 agcacttttt gtgcatcgtt ccccccatgg ccaatgctta gcagcgccga tgaaagcagt
     7621 tgcagccgcc ggaaccagga ttccgcctta tagggattgc ttcgctggaa aagattgacg
     7681 atcaggatcg tgatcaccag cgccaccaaa aaaccgatca gcggcgccag gatgataaag
     7741 aagaaggtgg gaatgacgct accgtaatcg atcacctgtt ttccgtgcgc tgtccagccg
     7801 cccgcgtggg caagggcggc cccgacaaag ccgcccagca gggtatggga ggagctggag
     7861 gggatgccga accaccaggt aaacagattc cagcagatgg ccgcgatcag cccggacagg
     7921 atcaccggca gggtgataaa atcgcggttt acgaaatgcg cgatcgtgtt gccgatcttg
     7981 aattccccga aataattgtt gaagatgaaa aaggcgccaa aattgaagat cgccgcccag
     8041 agcacggcct ggaagggtgt cagcaccttg gtcgagacga tggtggcgat ggagatagcc
     8101 gcgtcatgaa atccgttgat atagtcgaat atcagggcca gtacgatgat agtaagcaga
     8161 aggatcatgg tttgtgggct tgctggaagg acccggccgg gggcttggga aggaaccgga
     8221 ccgagtccgg tcaggcatat tttattataa tagattcgat cacattggtc gcgtcctcgc
     8281 atttgtcggt gacgatctcc atcacctggt agatatcccg ctttttgatc acctccttgg
     8341 cgtccggctc ggtctcgaag agacgttcga tgctcatgtc gaaaatatcg tccgcctggt
     8401 tctcgatgct gttgacccgg accagggcgt ccgtgatctt ccgcatatcc cgcatgttgc
     8461 gcaattccct taccgcaccc cggatctgcc gggctccctg ttcgatgaga tcggccattt
     8521 tctggatgcc ctggtcattg gggttcacct tgtaaaaatt gatctttttc gcggcggcgt
     8581 agatatagtc gcagatatcg tccagggcgc tggccagata gtggatgtct tcccggtcga
     8641 agggcgtgat aaaattgcgg ccgagctctg taaagatgcg gtgggtaagg tcgtcgttga
     8701 catgctcctg ggcttccaat tgggagatga tggccgcccg tttgtcgaaa tccggctcac
     8761 tgaccacatc ctttaataac tctcccatcc tggcgacacc gtccgccacc tcctcgaaaa
     8821 gggagtagaa gaccttgtct ttgggcatga acaattgaac aaatgcgttg aaacccttca
     8881 ttataatgac atgatcggta aaataaccga caagttagtc cgaatgtgga ccggcttcgg
     8941 gcgccggcct gtttggtaat gatttcttaa cattaagata aggaaacctg tccgcggtgg
     9001 gcaaaaaatg ggcctcatta atgaattatg ttttatctat cttcataact atttcaggaa
     9061 caggggtaaa aatgacctca ttttataggt gttgccccgt tttggtaagt gttaacatta
     9121 aggtaacatt ccggtaacat tggggtcatt gcataaacat tgttttgcag cgaattaacg
     9181 aacgtcgaca gcttaatcta aattttaacg aaccaagact tttatgaaac gcaacttcgc
     9241 aaaacaattc ctgttcatcg ttgctttttt ccttttgacc atcgcttcac aagcgcaggt
     9301 attcgtcaac aatgattccg ccttcagcgc aggaacaccc aaagcagggc gtctctgggg
     9361 ctatgccttc ggcgattttt attacaaatc acatagcgat tccctgaaca ggggtggcgc
     9421 caatcagtac accggtattc cgcaaagcag gaatgccttc gcgttccgtc ggatttatct
     9481 cggctatgat tataatttca acaagaaatt ctcctcggaa ttattgttgt cggctgaaga
     9541 taacttcccg gccggtaacc cgccttctac ggttgcatcc ggcagcagcg gcgacctgct
     9601 ggcaaacggc aaagagtcct tctttattaa gttgatgaac gtcaggatga aaaacatctg
     9661 gaagggaacc gacctggtgc tgggtgaaca gttcactcca tcctttgcca tgctgacaga
     9721 aaaggtctgg aactatcgtt cgatcgaacg caccatttcc gatatccgca ggaccccgtc
     9781 ctacgatctg ggtgttggcc tgaatggaac cttcgacccc gccaccaaga acttcggtta
     9841 taatttgttg atggctaccg gtaacagcga taaacccgct acgaacagct tcaaatggtg
     9901 gtatggcgac gtctggggca tgttcctcga taaacacctg atcctcgatg tctacgccga
     9961 ctaccaacgg ctcaactggc agccgggctg gcatcacgac cggcagatgc tcaaggcctt
    10021 tgcagcgtgg agctcgcagc ctatcaccgt tggcgtagag ggctttgtca acaatatggc
    10081 ggttgacacc aaggccagca agatcggtgg cggtgcagat accattgcta caatggccaa
    10141 tggtatagcc atgtttgttc atggggatat cattaccaac aagctccgtt tctttgcccg
    10201 ggtcgatctc tacaatccca attcgaaagt ggacaactcc aagtataatg cctattccgc
    10261 tctcggctct cccggtggct atatgacgcc cggctataag atgaacttca gccccagcac
    10321 cggctctccc gttacggcta caagcaccgg cgatcccacc gcgaaagaaa cgttcattac
    10381 tgcgggtctt gactttatgc cctttaagga cattcatctc gaaccgaacc tctggtatga
    10441 gcactacgct tctcagctgg ctaccggtac gaaggctgat aatgacgtcg tcttccgcct
    10501 caccttcttc tttgtattcg gcaagaacta taagaactcc tacacccagt tgtaattcta
    10561 acgttaacaa caaatatatt tatgaaaaag accatgccca tttttagagg caccctaacg
    10621 gtagctgtcc ttgtctttgg ctcgttattc gccagcatga gccttgtttc ctgcggtggc
    10681 ggtggcggca gcgccagcgg ttccgacagc gcggcttctg ccggcagcag tgacaatatg
    10741 cttacaggag cagggagttc ctttgataat cccttattct ccaagcaatt ttccgaatac
    10801 gacaaggcca acaatcaaaa gatcaactat cagtccgtgg gttcgggcgc gggtatctcc
    10861 cagctgacgg cgaagaccgt cgagttcggt gcttccgatg ccccgatgaa cggcaaacag
    10921 gattcggcgc tttccgcccc tgttgtgcat gttcccatta ccgagggcgc cgttgtcctc
    10981 agctataacc tgcctgaggt gaaggacacc ctgaagcttg tgcccggcgt gatcgccgac
    11041 atcttcctgg gcaagatcac gaaatggaac gacccgaaga ttgccgccga taacaaaggt
    11101 gtcaaactgc ccgctacggc gatcactgtc gcacaccgta gcgatggtag cggaacctcc
    11161 aacatcttta ctacctacct ttccaaggtg agcgaggatt ggagcaagac cgtcggcaaa
    11221 ggttcgtccg tcaattg
//
LOCUS       DATGFG010000912         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_456605, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000912 DATGFG010000000
VERSION     DATGFG010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456605"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..897)
                     /locus_tag="VKQ52_17265"
     CDS             complement(13..897)
                     /locus_tag="VKQ52_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89010.1"
                     /translation="MKQRKSDILWKVIMEEVFSDLLRFIFSDADQVYDMERGFEYLDK
                     ELAEMHPQPDEETDTRFADKLVKVYHRDGEEEWVLLHIEIQGDTTKRGEFSQRMYEYF
                     YRIRDRYRRPVSAIAVFIGKNGRDMPGRYTYEYRQTRLTYEYLAFSILDFSDEALDKS
                     NNPFAQVLLVAKTALLEGKIPEVDLFELKLLIANRLQEKGFGRVKTTAILNFLRNYVL
                     FDNPEMNRKFDEQIRSNDKYNAMNTIEYIRMEGKEEGLVTVVNNLITMTEFSDEKIAS
                     IAGVSVAFVKEIRQEKRK"
     gene            1127..1582
                     /locus_tag="VKQ52_17270"
     CDS             1127..1582
                     /locus_tag="VKQ52_17270"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HLZ89011.1"
                     /translation="MASPGARFQNIECPVDFVPMNENVARVNAFLVLVLVAVWWVTGY
                     DLIPALLAVDFAVRAANYGKFSPVNIAGGFLVKLLTIPTRLVDRAPKRFAALVGFIFS
                     AAISLLSLAQLPLAARVLASVLLFFAALEAFFRFCAGCYVYMLIKKLFP"
     gene            complement(1629..1961)
                     /locus_tag="VKQ52_17275"
     CDS             complement(1629..1961)
                     /locus_tag="VKQ52_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89012.1"
                     /translation="MAKYSLTYDAKDALAWDPEELKIEVSRILLDNGGDYLESPIVNM
                     ILFEDGKDRNELQFWNNLLLKSMKDDIYYYLCVVARTKEGDHFERNEGDPDLNDDYQQ
                     LLEDLESE"
     gene            complement(1996..2766)
                     /locus_tag="VKQ52_17280"
     CDS             complement(1996..2766)
                     /locus_tag="VKQ52_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLZ89013.1"
                     /translation="MDNAMLIQYCSDLHLEFPENREFLLKKPLVPKASILLLAGDIVP
                     FASLSAQDGFFDHLSKSFQRTYWIPGNHEYYGSDIRRRSGSFQENIRENVILTNNTVI
                     EEGEVRLILSSLWTNITPRFEKEIQQSLHDFHVIHHGTRKLRAAHVNALHVQSLDFIK
                     GELARPTEKKTMIVTHHVPTYFHYPEVYKDSPLNEAFAVELFPLIRDAAVSHWIYGHH
                     HFNRNDFQIGGTTLTTNQLGYVHKNENRGFRPDKTIEI"
     gene            complement(2965..3498)
                     /locus_tag="VKQ52_17285"
     CDS             complement(2965..3498)
                     /locus_tag="VKQ52_17285"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ89014.1"
                     /translation="MATTNWAIDPGHSKLQFKVKHLAIANVTGSFRTFGGAVQAAEDG
                     FNQAEVYFEMDAGSIDTNNPERDNHLRSNLFLDAAVHPRIAFSGVLTAGADTGTHTLS
                     GELSMHGVTRTVQLQVEHTGTGEGRFKDIRAGFEISGKVNRKDFGLDFNLLTEVGSLV
                     VGEEIRIQGDLELIRTA"
     gene            complement(3473..4126)
                     /locus_tag="VKQ52_17290"
     CDS             complement(3473..4126)
                     /locus_tag="VKQ52_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ89015.1"
                     /translation="MADLRQFCTRFSPLTEEAMDQLLQISTTKTYRKGRYLLKQGQTC
                     KHLFFINSGLVKSFFISGDKEFVMRFFTDNLLFSVFDSFITQTPSKFNLVALEETTVT
                     LINHDALEDLCNKHHCIETLFRKLVSVAAVKMTKRISEMLERDAAERYNQFVLENGDA
                     IQRISLGDIAKYLGITQPSLSRIRANHFLPYGKKPQAAPHLPLSKKQGKNGNNKLGN"
     gene            complement(4202..>4592)
                     /locus_tag="VKQ52_17295"
     CDS             complement(4202..>4592)
                     /locus_tag="VKQ52_17295"
                     /inference="COORDINATES: protein motif:HMM:NF023380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HLZ89016.1"
                     /translation="DETVIILLDNSGSGSLGEMSHKLAAIIYDKPYQLPEVLNEIKVE
                     DSILRSYVGAYELQPGFIITISFEKGALMAQATGQPKFELHAQTNTLFFLREVEAKLE
                     FVPGAQGKPTQLILHQGGRDMPGKRVN"
BASE COUNT          955 a         1153 c         1184 g         1300 t
ORIGIN      
        1 ccggcggaaa gattattttc tcttttcctg acggatttcc ttcacgaagg ctactgacac
       61 accagcaata gatgcgattt tttcgtcaga aaattcggtc atggtaatca gattgtttac
      121 aacagtaacc agaccttctt ctttgccttc cattcttata tattcgatgg tattcatggc
      181 gttatattta tcatttgacc tgatttgctc atcaaattta cgattcattt ccgggttatc
      241 aaatagcaca taattcctga gaaaattgag gattgccgtc gttttcaccc ttccaaaccc
      301 tttttcctgt aacctattgg caattaacaa cttcagttca aataggtcta cttctggaat
      361 cttcccttcc agtaacgccg tttttgccac aagtaagacc tgagcgaatg ggttattgct
      421 tttgtccagc gcttcatccg aaaaatctaa aatcgagaag gcaagatatt cgtaagtcaa
      481 tcttgtctgg cgatattcgt aggtatacct accaggcatg tctcttccgt tttttccgat
      541 aaagacggca atagctgata ctggccttct ataccggtcg cgtatgcggt aaaaatattc
      601 atacatccgc tgggaaaatt ccccccgctt agtggtatca ccctgtattt ctatgtgcaa
      661 aagcacccat tcttcttccc catcgcggtg ataaactttc accagtttgt cggcaaacct
      721 cgtatccgtt tcctcatcgg gctgcgggtg catttcagcc aattccttgt cgaggtattc
      781 aaatccgcgt tccatatcgt aaacctggtc ggcatcggaa aaaataaaac gcagtaagtc
      841 ggaaaacact tcttccatta tcaccttcca aagaatgtca ctctttcgct gtttcatagg
      901 tcaagtttta tcgttatacc attcgagccc ccaaggcttt tttgcatcga gggaatgcct
      961 ctttatacta acccgccttg gttttggaat agctcgaacg agtttacaaa ggtaagaata
     1021 agcaatcgct cagagaaacg caaccctcga aaatctatca gtctatcaaa atagtagatt
     1081 gaatgaaaaa aattacctat cttgcgtcga caaaacaagt tgatttatgg cctcccccgg
     1141 tgcgagattc caaaacatag aatgccccgt cgacttcgtt ccgatgaacg agaatgtcgc
     1201 acgcgtcaat gcctttttgg tgctggtgtt ggtagcggtg tggtgggtca cggggtatga
     1261 cctcatcccg gcgttgctgg cggtggactt tgccgttcgg gcggccaatt atggcaaatt
     1321 cagccccgtc aatatcgcgg gcggctttct cgtaaaattg ttgacgattc ccactcgttt
     1381 ggtggaccgc gcccccaagc gtttcgcggc gctggtaggg ttcattttct ccgccgccat
     1441 atccctttta tcccttgccc aattgccgct ggcggccagg gtcctggcct ccgtattatt
     1501 gttcttcgcg gcgctcgaag ccttctttcg cttctgcgcg ggatgttatg tatatatgct
     1561 gataaagaaa ttgttcccct gatagccgcg ggcgtagccg cctgggtagt cggggaccgg
     1621 gcgcggcgct actcactctc cagatcttcc aacaactgct gatagtcatc gttcagatcg
     1681 gggtcacctt cattgcgctc gaagtggtcg ccctccttcg tccgggcaac gacgcagaga
     1741 taatagtaga tgtcgtcctt catgcttttc agcagcagat tgttccagaa ttgaagttca
     1801 ttcctgtctt tcccatcctc gaagaggatc atgttgacga tcgggctttc caggtagtcg
     1861 ccgccgttgt cgagcaggat gcgcgatact tcgatcttta gttcttcggg gtcccacgcc
     1921 aaagcgtcct tggcgtcata tgtcaggctg tatttggcca tagtcgttcc tatattgaat
     1981 ggttgcaaaa gtacatcata tttcgatggt cttgtccggc cggaaacccc ggttttcgtt
     2041 tttgtggaca tagcccaact gattggtagt aagcgtcgtg ccgccgatct ggaaatcatt
     2101 cctgttgaaa tgatggtggc catagatcca atgcgagacc gcagcgtcac ggatcagggg
     2161 gaagagctcc accgcgaagg cttcattgag gggactgtcc ttgtacactt ccgggtaatg
     2221 aaagtaggtg ggaacatgat gggtgacgat catcgttttc ttttccgtcg gccgggccag
     2281 ttcgcctttt ataaaatcaa ggctttgcac gtgtaacgcg ttgacgtggg ctgcccgcag
     2341 cttgcgggtg ccgtgatgga tcacatgaaa atcatgcagg ctttgctgga tctccttttc
     2401 aaaccttgga gtgatgttgg tccagaggct cgacaggatc agccggacct caccctcttc
     2461 gatgaccgtg ttattggtta gaatgacgtt ctccctgata ttttcctgga aggaacccga
     2521 ccggcggcgg atatcgctgc catagtattc gtgattgccg ggtatccagt aagtgcgttg
     2581 gaagctcttt gaaagatgat caaagaagcc gtcctgcgcc gacagcgatg cgaacggaac
     2641 aatgtctccc gccaatagca ggatactggc tttgggaacc agtggttttt tcaataggaa
     2701 ctccctgttc tccgggaatt cgaggtggag gtctgagcag tattgtatta acatggcgtt
     2761 atccaaaggg ctatgggcta atttacgcca agttgttgag gatcagttta attaaatttt
     2821 acccattttt ggggttagtg gatggccttt ttttatggtt ccgttcgctc aggtcgtctc
     2881 ttgtggttcc gttcgcgcag accgtctctt atggctccgt tcgccccggt cgttcgcccg
     2941 ggccgttagc tcagcccgtg cggctcaggc cgtgcggatc agctccagat caccctgtat
     3001 cctgatctcc tcccccacaa cgaggctgcc tacctcggtc agcaggttga aatcgaggcc
     3061 gaaatccttg cggttgacct tcccgcttat ttcaaaaccc gcccggatat ccttaaacct
     3121 gccctcgccg gtgcccgtgt gctccacctg cagctgaacg gttcttgtca ccccatgcat
     3181 ggacagctca ccgcttaggg tatgcgtacc ggtatcggct ccggccgtca ggactccgga
     3241 aaaagcgatc ctggggtgta ccgccgcatc caggaacagg ttcgaacgca ggtgattatc
     3301 cctttccgga ttgttggtgt cgatgctgcc ggcgtccatt tcgaaataga cttcggcctg
     3361 gttgaatcca tcctccgccg cctgtaccgc gccgccaaag gtcctgaagc tgcctgttac
     3421 gtttgctatt gcgaggtgtt tgaccttgaa ttgcagtttt gaatgtccgg ggtcaattgc
     3481 ccaatttgtt gttgccattt tttccttgtt ttttagacaa aggtaaatgg ggcgcggcct
     3541 gtggtttttt accatagggt aaaaaatggt tggcccttat ccggctcagg gatggctggg
     3601 tgatcccgag gtatttggca atgtcgccga ggctgatgcg ttgaatggcg tcgccgttct
     3661 ccagcacaaa ttggttatag cgttccgcgg catctctttc caacatctca ctgatgcgtt
     3721 tcgtcatttt tacggccgcg acggagacca gttttctgaa cagggtttct atgcagtggt
     3781 gtttgttgca caggtcttcg agcgcgtcgt gattgatcag ggttaccgtc gtctcttcca
     3841 acgcgacgag gttgaatttg gacggggtct gcgtgatgaa gctgtcgaac acggagaata
     3901 gcaggttgtc ggtaaagaag cgcatcacga attccttatc cccactgatg aaaaaggatt
     3961 tgaccaggcc gctgttgatg aagaagagat gtttgcaggt ttgcccctgc ttcaggagat
     4021 accggccctt gcggtaggtt ttagtggtcg agatctgcaa cagctggtcc atcgcttctt
     4081 cggtgagcgg ggagaaccgg gtgcagaatt gacggagatc ggccatagta ggtgaaggta
     4141 gccctttttg gttatcccgt ccgccgtgcg ttgggggctt atccggcatg cgttgcggct
     4201 cttagtttac ccgtttcccc ggcatatccc gtccaccctg gtggaggatg agctgggtgg
     4261 gcttgccttg cgcaccgggg acgaattcca gctttgcttc gacttctctg aggaagaaaa
     4321 gggtgtttgt ctgtgcatgc agctcgaatt tcggctggcc ggtggcctgg gccatgaggg
     4381 cccccttctc gaagctaatg gttatgatga agccgggttg gagttcatat gcgccaacat
     4441 aggatcgcag gatggagtct tcgactttga tctcgtttag tacctcgggt agctggtagg
     4501 gcttgtcgta gatgatggcc gcaagtttgt gactcatctc gcccagactg cctgagcccg
     4561 agttgtccag caggatgatg actgtttcgt cg
//
LOCUS       DATGFG010000913         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1330190, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000913 DATGFG010000000
VERSION     DATGFG010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330190"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VKQ52_17300"
     CDS             complement(<1..259)
                     /locus_tag="VKQ52_17300"
                     /inference="COORDINATES: protein motif:HMM:NF025651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YWFCY domain-containing protein"
                     /protein_id="HLZ89017.1"
                     /translation="MQSGDNLQALRKNLDFIRFGSILILLIHYYSACYPAWQKWNLTA
                     SIVNQVIGSLCRGVFFMSGINGPKLASLFLLAISLLGEKGKK"
     gene            complement(298..1527)
                     /locus_tag="VKQ52_17305"
     CDS             complement(298..1527)
                     /locus_tag="VKQ52_17305"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HLZ89018.1"
                     /translation="MVAKIKARKSISKALNYNEAKVRAGVGQLLLASGFGCDVAELNF
                     HEKLRRFELLNQRNDWIETNTLHIFLSFPPGEDLAQELLQRLSLDYMDRIGFGEQPFL
                     VYQHHDTRHAHIHIVTTRIRPDGEAINIHNIGQYVSDPVREALEYEYGLTRARGRQAT
                     PSDTEPPDLASKVQEVTGTYKFTSLDELNVILAQNGMTAWPGTPGSAIRSNRGLLYSR
                     IDADGNRTGAPIKSSDLVTRPTLAWLEKRFELNKVRKLACKDRIVEVLAGALASKVAA
                     PASILGRLQQRQISVQPRRDHDGNLESVYVVDNRNKSVFNAEELGFAPETLLTRLGLP
                     LLPGPAIEAAGQKSGVSKNKPGQPAKPSLGTPSISRGQTAIAAQVAFLLHSSQTGMPG
                     PAGDEPPKKKKRKKRSL"
     gene            complement(1512..1916)
                     /gene="mobC"
                     /locus_tag="VKQ52_17310"
     CDS             complement(1512..1916)
                     /gene="mobC"
                     /locus_tag="VKQ52_17310"
                     /inference="COORDINATES: protein motif:HMM:NF039635.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HLZ89019.1"
                     /translation="MPRKKTPQNKLLKYRVTTKIDESTYLKLRRLLDENPHNDMSRVV
                     RAVLINRPIRVFTRDLTLDNLMEELARVRTELKHIGVNINQITKKFNTYPEPQRKAFY
                     AKLAFQEYLAIESQIDRLLEIITPLSKKWLQK"
     gene            complement(2141..2590)
                     /locus_tag="VKQ52_17315"
     CDS             complement(2141..2590)
                     /locus_tag="VKQ52_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89020.1"
                     /translation="MSNYNEYLHSDFKKLGFPNYIKMLDTELRLKDVSFLFTRENLPT
                     APFFGKYDAEFRFAIAHPSEDSGKRPFIAMIQTSLFHASIQDRQQAMLHSQFNYWPVP
                     DRQALIADIHQALEDHLRQSLSVSDRITAANRMHQGETLNAHVKKRC"
     gene            complement(3115..>3598)
                     /locus_tag="VKQ52_17320"
     CDS             complement(3115..>3598)
                     /locus_tag="VKQ52_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89021.1"
                     /translation="PFLDPEDIELRNLSDDPGLEKEFSDLGFDLQSSQLVHSGSAGPF
                     TVLTHHYPLGKTEEPLYLLEIRFLIGHLDRVGDVILIGEENNTDHRIIAIQAACINAL
                     DITDLSPRFVYEKTYAIGHGSLPNRSSILADILQGQRSSMQQLQNILKFFQALKAMES
                     "
BASE COUNT          765 a          769 c         1122 g          942 t
ORIGIN      
        1 cctttttccc tttttcgcct agaagggaga ttgccagtag gaagagggat gcaagctttg
       61 gtccattgat gccggacata aagaataccc cgcggcagag gctgccgatg acctggttaa
      121 ctatggatgc ggtcaggttc cacttctgcc atgcaggata gcaggcagaa taataatgaa
      181 tgagaaggat caggatactg ccgaatcgaa tgaagtctag gttttttcgg agcgcctgca
      241 ggttgtcgcc gctttgcatg gtgagtgggt tggtgaggaa atggggtgaa atccggctca
      301 gagagatctt ttcttgcgtt tcttcttttt gggaggttcg tcgccggcag gcccgggcat
      361 tccggtctgg cttgagtgga ggagaaaggc gacctgcgcg gcaatggcgg tctgtcctcg
      421 ggatatagat ggcgtgccga gggacggctt agccggctgg cctggcttat ttttactgac
      481 gccgctcttt tggccggcgg cttcaattgc agggccaggg agtagcggaa ggccaagccg
      541 agtaagcagc gtttccgggg caaagcctag ttcctcggca ttgaagacag atttgttgcg
      601 attgtcgaca acgtagacgg attccaggtt accatcgtga tcccgacgcg gctggacact
      661 gatctggcgt tgctgtaggc ggccgaggat ggaagcagga gcggctactt tgctggcgag
      721 ggcgcccgcc aggacctcga ctatccgatc cttgcaggct agcttccgaa ccttgtttag
      781 ctcgaagcgt ttttctaacc aggccagggt gggccgggtg acgaggtcgc tggatttgat
      841 cggagcgcct gtgcggtttc cgtcggcgtc gatccggctg tagaggagac cgcggttgct
      901 gcggatagca ctgccgggag taccgggcca ggcagtcatt ccattttggg ccagaataac
      961 gttaagttcg tccagggatg tgaatttgta ggtgccggtg acttcctgga ccttggaggc
     1021 aagatcgggc ggctccgtgt cgctgggagt ggcttgccgt ccccgggcgc gagtaaggcc
     1081 gtattcatat tcgagggcct cgcggacggg atcggagacg tactgaccaa tattgtgaat
     1141 gttgatagct tcgccgtccg gcctgattcg tgtcgtaacg atatggatat gagcgtgtct
     1201 ggtatcgtgg tgctgataga ccaagaaggg ttgctcgcca aagccaatgc gatccatgta
     1261 gtctagtgac agtcgctgga ggagctcctg ggcaagatcc tctcctggcg gaaaactgag
     1321 aaagatatgt agggtattcg tttcgatcca atcattacgt tgattgagca attcaaagcg
     1381 acggagtttt tcatggaagt tgagctcggc gacatcacaa ccgaagcctg aagcaaggag
     1441 gagctgccct actcccgcgc gtactttggc ctcattgtaa ttgagagcct tgctgatcga
     1501 ttttctagct tttatttttg caaccatttt ttgctaaggg gggtgatgat ctcgaggagg
     1561 cgatcaatct gtgattcgat ggcgaggtat tcctggaatg cgagctttgc atagaatgcc
     1621 ttgcgctgag gttcgggata ggtattgaac tttttagtga tctggttgat attaactcca
     1681 atgtgcttga gctcggttcg gacgcgggcg agctcttcca tgaggttgtc gagtgtgaga
     1741 tcacgggtga aaacccggat ggggcggtta ataaggacgg cacgaacgac gcggctcatg
     1801 tcgttatgtg gattttcgtc caggagacgt cggagcttga gataggttga ctcgtcgatc
     1861 ttggtagtga cccggtattt aaggagtttg ttttgcggtg ttttctttcg gggcatgggt
     1921 gcatatatat attatggagt gtttttcggc aggtgatcta cgggcaggcg gatacgaaaa
     1981 tggttttggt ggacatttat acatcttgcc gggcgcgata aggcgcgccc tccccccaag
     2041 aaagacttgc tgcgctccgc gcgtagcgtc cgccctggcg ggcggcaagg aaggggtatt
     2101 atgtcgaatt ggggttattt gatgccacgc cagaagacgg tcagcatcgc ttttttacgt
     2161 gggcgttgag agtttctccc tgatgcatgc gattagcagc ggtaatccga tcggaaacgg
     2221 aaaggctttg acggaggtga tcctcgagag cctggtgaat gtctgcgatg agtgcctggc
     2281 ggtcgggcac gggccagtaa ttaaactggc tgtgcagcat cgcctgttgt cggtcttgta
     2341 tagaggcatg gaacagactg gtttggatca ttgctataaa aggtcgcttg ccgctgtcct
     2401 ccgatggatg ggcgattgca aagcggaatt cagcgtcata tttcccaaaa aacggggcgg
     2461 tcggtagatt ttcccgtgtg aaaaggaagc tgacgtcttt caggcggagc tcggtgtcca
     2521 gcatttttat gtaatttggg aatccgagct ttttgaagtc gctatggagg tattcattgt
     2581 agttggacat ggtaggcaga attgaaggtg ggatagccag tgggtgactt tggaggcgct
     2641 ggtcagcggg actacgtcgt ttttgacaag ggcttagtct ggtcagctgg tctttctcct
     2701 agcgaaagtt ggagtagctt ggtaggctgg attgtcgtgg ccaagaggtt gagtgtgtac
     2761 ccatccgggc cgagaagatc tggaacaagt ctttttatcc atgcaatctc tgtactatcg
     2821 agccaacggt cagtctgggc cttcccgtcg aagatcaccc atttactgaa aaccttggag
     2881 atgaggaatt cctgcggttc gatcacaggg aggcgtttat tcgacggtag ggcagagatt
     2941 tgcaggccag tcagaatctt agcctcttcg agaagtccgg cgaaatattt cttgatcctg
     3001 tcaatgtcgt cagattcgag agtttggtca gattggccgg tttcgtcgaa tatggaccaa
     3061 gtcgttcgga atttgcaaat gtaatacatg ggtaccgtct tagatatctg ttgattatga
     3121 ctccatggct ttcaaggcct ggaagaattt gagaatgttt tgaagctgct gcatgctgga
     3181 gcgttggccc tgaagaatgt ctgccagaat ggaggacctg ttcggcaggg agccgtggcc
     3241 tatggcgtaa gtcttttcat atacgaaccg cgggctgagg tcagtaatat ccagagcatt
     3301 gatgcaggct gcctgaatgg cgatgatgcg gtggtcggta ttgttctctt cgccgataag
     3361 tataacgtct cctaccctgt cgaggtggcc aattaaaaac ctgatttcga gaaggtaaag
     3421 aggttcctcc gtcttgccaa gtgggtaatg atgtgtaagc acggtaaaag gtccggctga
     3481 gcctgaatgg acaagctggc tgctttgcag atcaaaccca aggtcgctga attccttttc
     3541 aagaccagga tcatctgaaa gatttcgtaa ttcgatgtct tcgggatcca gaaagggc
//
LOCUS       DATGFG010000914         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1534986, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000914 DATGFG010000000
VERSION     DATGFG010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534986"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..463)
                     /locus_tag="VKQ52_17325"
     CDS             complement(77..463)
                     /locus_tag="VKQ52_17325"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HLZ89022.1"
                     /translation="MILPSLFIAPTDGMGRGVFTSEDIAKDTIIEVSPVIVMTGEERK
                     LLDQTLLHDYIFEWGDRREKCCMALGYIPLYNHSYKSNCEYEMDFQQRLITIKTVRFI
                     KAGEEVFINYNGDWNNAHPVWFDRQP"
     gene            500..1888
                     /gene="mgtE"
                     /locus_tag="VKQ52_17330"
     CDS             500..1888
                     /gene="mgtE"
                     /locus_tag="VKQ52_17330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HLZ89023.1"
                     /translation="MSLEAENIDVREQFLEVIQSEDVLYIREFLNNQNISDVAELIYE
                     YPEYESQIIGHLSIHRASSTFKILDVPTQKRIIQELPAFKTAELLNDLSADDRTSFLE
                     ELPSEVVKELIKTLDPEERKITLSLLGYPEGSVGRLMTPDYIAVQKDWTVREVIDHIR
                     EVGKDSETIDVIYVVDDKGVFIDDVRIREIILANPDKEIKDVIDNRYIALNVNDEQEA
                     ASEAFRMNNRVALPVLDNNKILLGIVTIDDVLWITQEESAEDITKIGGTEALDEPYLE
                     IPLIKLFKKRVVWLVVLFLSEMLTATAMGYFEDEIAKAVVLALFVPLIISSGGNSGSQ
                     ASTLIIQAMALGEITIADWWRVIRRELVSGMLLGAVLGVIGFTRVMLWNQIFHTYGQH
                     SFLIASTVGFSLVGVVLWGTIAGSMLPIVLKRFGADPATSSAPFVATLVDVTGLVIYF
                     SVAFLIMRGILL"
     gene            2048..2923
                     /locus_tag="VKQ52_17335"
     CDS             2048..2923
                     /locus_tag="VKQ52_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89024.1"
                     /translation="MRYIKQKMTVIALLLLLVGTAGYSQRGGGGRGFSGGGGGRSFSG
                     GGSRGFSGGGASGFSGGGARGFSGGGGYRGFSGVSRGSVGGAGYGGRRYSGAGIHAYG
                     SGYRGGYSGYRGGYYGHGYGGGRGYYGGYYGRGYYGGWGYGGWGWGLGLGIGFGLGYD
                     WPYYGYPYGYYGYPNYYGYPYSPYAYPPPASYDPYYDDPEDPYGDGSGQRQEPRRNNR
                     PDNYDDNNYNYPNPDSLQNRPGTNNHPSSYSGDYGSTASAAGDKVWVKAHWKHTNDGW
                     IWIEGYWKAQPAGNQ"
     gene            3057..>3854
                     /locus_tag="VKQ52_17340"
     CDS             3057..>3854
                     /locus_tag="VKQ52_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89025.1"
                     /translation="MCGIVAYIGPREAYPIILKGLKRLEYRGYDSSGVALLNNDLRVY
                     KKKGKVSELEDSLVGKDLHAHAGMGHTRWATHGEPNDRNAHPHHSASGRLAMIHNGII
                     ENYTQLKQELSRKGYHFHSDTDTEVLLNFIEDIQKNNQCGLEEAVRIALKRVVGAYVI
                     VLLDRDNPDTVIAARKGSPLVIGIGKGEHFLASDASPIIEYTKEVVYVNDYELAILKA
                     DELILKNLGNEKITPFVQKLDLEMAAIEKGGYEHFMLKEIFEQPDTIF"
BASE COUNT          856 a          953 c         1142 g          903 t
ORIGIN      
        1 ttggccaggt gcagatgaac agcgtgggga tcaattttgc gctgaagttc tcgccggtta
       61 ccagccgcag gcggcgttaa ggctgccggt cgaaccagac agggtgcgca ttgttccagt
      121 cgccgttgta gttaataaag acctcttcgc ccgcctttat gaaccggacg gtttttatcg
      181 taatgagccg ttgctgaaaa tccatttcgt attcgcagtt ggacttgtag gagtgattgt
      241 acagcgggat atagccgagc gccatacagc atttttcgcg gcgatcgccc cactcgaaga
      301 tataatcatg caataaagtc tggtccagca atttgcgttc ttcgccggtc atgacgatga
      361 cgggggagac ctcgatgatc gtatctttag cgatgtcctc cgaagtgaag actccccttc
      421 ccatcccatc cgttggcgcg ataaatagtg acggcaagat catgcgggca atttaacatt
      481 tttacgtagg tttggtgcca tgtcgttaga agcggaaaat atcgatgtca gggagcagtt
      541 cctggaggtg atccaatcgg aggatgtgct atatataagg gaatttctga ataaccagaa
      601 tatcagtgat gtcgccgaac tcatctatga atatcccgaa tatgagagcc agatcatcgg
      661 ccacctgagc atccaccggg cttccagcac attcaagatc ctggatgtcc cgacgcagaa
      721 gcggatcatc caggaattgc ctgccttcaa gaccgccgaa ctgttgaacg acctctccgc
      781 ggatgaccgg acctcctttc tcgaagagct gcccagcgaa gtggtgaagg agttgatcaa
      841 gaccctcgac ccggaggaaa gaaagatcac cctgtcgctg cttggttacc cggagggaag
      901 cgtcgggcga ttgatgacac cggactatat tgccgttcag aaggactgga cggtgcggga
      961 ggtgatcgat catatccggg aagtgggcaa ggatagcgag accatcgacg tgatctatgt
     1021 cgtagatgac aagggagtgt ttatcgacga cgtccggatc cgggagatca tcctggccaa
     1081 cccggataaa gaaataaaag atgtcatcga caaccggtat atcgccctga atgtcaatga
     1141 cgaacaagag gctgcaagcg aggcattccg aatgaacaac cgcgtggcgc tacccgtgct
     1201 ggacaataac aagatcctgc tggggatcgt gaccatcgat gatgtgttgt ggatcaccca
     1261 ggaagaatcc gctgaagaca tcaccaagat cgggggtacc gaagccctcg acgagcccta
     1321 tcttgaaatt cccctgataa aactttttaa gaaacgcgtg gtctggctgg ttgtgctttt
     1381 cctcagtgaa atgctcacgg ctacggccat ggggtatttc gaggatgaga tcgcaaaggc
     1441 cgtcgtgctg gcgctcttcg tcccgctgat catttcgagc ggcggtaaca gtggcagcca
     1501 ggcttccacg ctgatcatcc aggccatggc gctgggggag atcacgatcg ccgactggtg
     1561 gcgggtgatc cggcgcgaat tggtgtccgg gatgctgctg ggggccgtgc tgggtgtcat
     1621 cgggtttacc cgggtcatgc tctggaacca gatctttcac acctatggcc aacactcctt
     1681 cctgatcgcc agtacggtcg ggttttccct tgtgggagtg gttttgtggg ggaccattgc
     1741 cggtagtatg ctgccgatcg tcctgaagag attcggcgct gaccccgcaa cgtcctcggc
     1801 gccgtttgtc gctaccctgg tcgatgtaac agggctcgtg atctatttct ccgtggcctt
     1861 cctcatcatg cgggggatcc ttctttaatc aggatcgtcg gcgcagccgt cacgagcttg
     1921 taactaactg tttgttaatg atgtgcgcgc attagttaat gtactttttt cacctgtttt
     1981 tttgtcgtaa attaaactat gggtccgcgg aggagtctaa cacgggtacc ttattaaaaa
     2041 gttaattatg cgttacataa aacaaaaaat gaccgttatt gcgctgctcc tgctactggt
     2101 gggaacggcg gggtattccc agcgtggcgg tggcggccgg gggttttccg gcggcggcgg
     2161 tggaagaagt ttctccggcg gaggatccag gggattttcc ggtggtggcg ccagcgggtt
     2221 ttcgggcggt ggggcgagag gattttccgg tggcggcgga tacaggggtt tttcgggtgt
     2281 gtcgaggggc tcggttggtg gagccggcta tggcggcagg cgttatagcg gcgcgggcat
     2341 ccacgcctat gggtccggtt atcgcggtgg ctattccggt tatcgcggtg gctattatgg
     2401 tcatggctat ggcggcggcc ggggttacta cggcggctat tatggccgtg gttattatgg
     2461 cggctggggt tacggcggtt ggggctgggg tctcggcctc gggatcggct ttggcctggg
     2521 ttatgactgg ccttattacg gttatcctta tgggtattac ggctatccca attattatgg
     2581 ctatccttat tcgccttatg cataccctcc ccctgccagt tatgatccgt attacgatga
     2641 cccggaggat ccctatgggg atggttcggg gcagcggcaa gagccccgac gcaacaacag
     2701 gcccgacaat tacgacgaca acaactacaa ctatcccaat cccgattcgc tgcagaaccg
     2761 gccgggtacc aataatcacc cctcctccta ttccggcgat tatggctcaa cggcttcggc
     2821 cgccggtgac aaggtttggg tgaaggcaca ttggaaacat accaatgacg ggtggatctg
     2881 gatcgaagga tattggaagg cacagccggc cggcaaccag taatgttcat tgtacactta
     2941 tacctgcgcc cccttctgac ggagggggca ttttttttgc atcgccggaa gagtccccgg
     3001 ccctttttgg ttttggatat tattgtctaa tattgccctg ctaaactttt gcaattatgt
     3061 gcgggatcgt cgcttatatc ggaccacgag aggcttatcc aattatcctg aagggattaa
     3121 agcgcctgga gtaccggggc tatgacagtt cgggggtcgc cctgctcaac aacgacctgc
     3181 gggtgtacaa aaagaaggga aaggtgagcg aactcgagga cagcctcgtc ggcaaggacc
     3241 tgcatgctca tgccgggatg gggcataccc ggtgggcgac gcatggcgag ccaaacgatc
     3301 gcaacgcaca cccgcatcat tcggccagcg gccggcttgc catgatccac aatggcatca
     3361 tcgagaacta tacccagctg aagcaggaac tctcgcggaa ggggtatcat ttccacagcg
     3421 ataccgacac cgaagtattg ctcaatttta tcgaagacat ccaaaagaac aaccagtgtg
     3481 ggctggaaga agccgtgcgc atcgccctga aacgggtggt cggcgcctat gtgatcgtgt
     3541 tgcttgaccg ggacaacccc gataccgtga tcgcggcccg gaagggaagc ccgttggtga
     3601 tcgggatcgg gaagggcgag catttcctgg cttcggatgc ctcgccgata atagagtata
     3661 cgaaggaagt tgtttatgtc aatgactatg agctggcgat attgaaggcg gatgagctga
     3721 tcctgaagaa tctggggaac gagaagatca cgccattcgt tcaaaaactg gacctggaga
     3781 tggcagccat cgaaaagggc ggttacgaac acttcatgct gaaagagatc ttcgagcagc
     3841 cggataccat cttc
//
LOCUS       DATGFG010000915         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_195341, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000915 DATGFG010000000
VERSION     DATGFG010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195341"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1150
                     /gene="rlmN"
                     /locus_tag="VKQ52_17345"
     CDS             101..1150
                     /gene="rlmN"
                     /locus_tag="VKQ52_17345"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HLZ89026.1"
                     /translation="MATIKEKKNIRHLSLSELEQYFETLGEKKFRVTQVWEWLWQKHA
                     HSFDEMTNLSKELRHKLSENFSFPALAVDATQYSADGTIKSRFRTADHHLVEGVLIPT
                     DERKTACVSSQIGCSLSCKFCATGYMDRKRNLDYDEIYDEVVLINQQSERVYDKKLTN
                     IVFMGMGEPLLNYKNVLKAIEKITSPDGLAMSPRRITVSTAGVAKMIRQLGDDKVKFK
                     LALSLHAANDRKRNEIMPINESNNIKALIEALNYFYQQTGNEITFEYILFQNFNDSRQ
                     DADELIKIYRQVPADLINIIEYNPIDQAAFAKPDEDAVQQFMQHLEKNRVNARLRRSR
                     GKDIDAACGQLANKE"
     gene            1157..1732
                     /locus_tag="VKQ52_17350"
     CDS             1157..1732
                     /locus_tag="VKQ52_17350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ89027.1"
                     /translation="MDATLLLQNITRHITLSPEEQAHFTSILHYRAVKKKQFLTREGE
                     ITRGPVFVTSGILRSYSIDANGYEHVIQFAPPGWWVGDIGSMIHQRPSILSVDALEDS
                     EVLYIAEADIEDIFERIPKFERFFRILSQNALASHQARLVSILSLPAKDRYAEFCRLY
                     PSLIDCLPQKQIASYIGVTPEFLSKMLNTRA"
     gene            1847..>2343
                     /locus_tag="VKQ52_17355"
     CDS             1847..>2343
                     /locus_tag="VKQ52_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HLZ89028.1"
                     /translation="MKKRIERIISQPAAPGMVGDGFRVYNYIPGGDRPASAIPQQRIS
                     PFLMLDFNAEIDFGPSDHVRGVGVHPHKGFETVTIAYKGSVAHHDSAGNSGVIKSGDV
                     QWMTAGAGILHKEYHEEAFSKKGGPFEMVQLWVNLPKKDKLTAPHYQAITADQISKLI
                     LPGNQG"
BASE COUNT          596 a          814 c          524 g          409 t
ORIGIN      
        1 gaactcctca ccaacggcgc ccccaacggc tccgtcaacc gccgcagctt cctcctgccc
       61 tggtagcggt ccccccaaaa aatcgtacct ttgcgcccaa atggcaacga taaaggagaa
      121 aaagaacatc aggcacctca gcctgagtga gttagagcaa tacttcgaaa ccctgggcga
      181 aaaaaagttc cgggtaaccc aggtctggga atggctatgg caaaaacacg ctcatagctt
      241 cgacgaaatg accaacctca gcaaagagct ccgccacaag ctctcggaga atttctcctt
      301 tcctgccctc gcggtcgacg ccacccaata ctccgccgac ggcaccatca agagccgctt
      361 ccgcacagcc gaccatcatc tggtggaagg cgtcctcatc cccaccgacg agcgcaagac
      421 ggcctgcgtg tcctcgcaaa tcgggtgcag cctcagctgc aagttctgcg ccacaggcta
      481 catggatcgc aaacgcaacc tcgactacga cgagatctat gacgaggtgg tcctcatcaa
      541 ccagcaatcc gaacgcgtct acgacaagaa actcaccaac atcgtcttca tgggcatggg
      601 cgaacccctg ctgaattata agaacgtcct caaggcgatc gagaagataa cttcacccga
      661 cggtctcgcc atgagccccc gccgcatcac cgtctctacc gccggcgtgg caaaaatgat
      721 caggcaactc ggcgacgaca aggtgaaatt caagcttgcc ctctccctcc atgccgccaa
      781 tgatcgcaag aggaacgaga tcatgcccat caacgagagc aacaacatca aggccctcat
      841 cgaagcgctg aactattttt accagcagac cggcaacgag atcaccttcg aatacatcct
      901 cttccagaat ttcaacgaca gccgccagga tgccgacgaa ctgataaaga tctaccgcca
      961 ggttcccgcc gatctcatca atatcatcga atacaacccc atcgaccagg ccgccttcgc
     1021 caaacccgac gaggatgccg tccagcaatt catgcaacac ctcgagaaga accgggtcaa
     1081 cgcccgcctc cgccgcagcc gcggaaagga tatcgacgcc gcctgcggcc aactcgccaa
     1141 caaagagtaa tcatccatgg acgccaccct cctcctccag aatatcaccc gtcatatcac
     1201 cctcagcccg gaggagcagg cccatttcac gtccattctc cactaccgtg cggtaaaaaa
     1261 gaagcaattc ctcacccgcg aaggcgagat aacccgggga cccgtcttcg tcaccagcgg
     1321 catcctgcgc agctattcca tcgacgccaa cggctacgaa catgtgatcc agttcgcgcc
     1381 cccaggctgg tgggtcggcg acatcggcag catgatccat caacgtccct ccatcctcag
     1441 cgtggacgcc ctcgaagaca gcgaagtact ctacatcgcc gaagccgata tcgaagacat
     1501 ctttgaacgc atccccaaat tcgaacgttt cttccgcatc ctctcccaaa acgccctcgc
     1561 ctcccaccag gcccgcctcg tcagcattct cagcctccct gcaaaagacc gctatgccga
     1621 attctgccgc ctctatccct ccctcatcga ctgcctcccc caaaaacaaa tcgcttccta
     1681 catcggcgtc actcccgaat tcctgagcaa gatgctcaac acccgcgcct gatcctgatc
     1741 ctgcctgccc ggagcccgga cccatcctct gcacccgcgc ttaatctagg ttattgtggt
     1801 cccccctccc cctatcgtac ctttgcttca tcaaggagaa aagaatatga aaaagagaat
     1861 agaacgcatc atctcacaac ccgccgcccc cggtatggta ggcgacggct tcagagtata
     1921 taattatatc cccggcggcg accggccggc cagcgccatc ccgcaacagc ggatcagccc
     1981 cttcctcatg ctcgacttca atgccgagat cgacttcggg ccatccgatc acgtgcgcgg
     2041 cgtcggcgtg catccgcata aaggttttga aacggtcacc atcgcctata aaggcagcgt
     2101 cgcccatcac gacagcgcgg gcaacagcgg tgtcatcaaa tccggcgacg tgcaatggat
     2161 gacggcaggt gcgggcatcc tccacaagga ataccacgaa gaagccttct cgaagaaggg
     2221 cggccccttc gaaatggtcc agttgtgggt caatctcccc aaaaaggata agctcacagc
     2281 gcctcactat caggcgatca cggccgatca gatcagcaaa ctcatactgc ccggcaacca
     2341 ggg
//
LOCUS       DATGFG010000916         5260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1075604, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000916 DATGFG010000000
VERSION     DATGFG010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075604"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VKQ52_17360"
     CDS             complement(<1..1026)
                     /locus_tag="VKQ52_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89029.1"
                     /translation="MKCTPITPEAANAARQFATACRDHYMLLRRTIATLSQPSIPGGA
                     LTNSNSLTNLNSSINLNSLTAIHLLIPPIFPDGATAIPPSLTRLNACLSPDRNLSTLI
                     HRLAPRHDPTRELFYGLHRENQLVSATIGRTITEYNQYRKDFFDNFYTLVQLHDELAA
                     FVKGKTLSPAAGIWVEGYFRIFRNWVQDGGAKDIAPLLEKIMIPVINLNCNNGGSPFI
                     HRSSTAALQARFAAQNIAALVQNLCIRLDQHATIYKNAIRITGCTNRHLNTPRSTTKT
                     RHNSKALQNTKLFENGKIVPNTKPFRNTKLFPNSKPFRNTKLHNTLRTIFNPQNIRLH
                     KNYLLATA"
     gene            complement(1033..2895)
                     /locus_tag="VKQ52_17365"
     CDS             complement(1033..2895)
                     /locus_tag="VKQ52_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89030.1"
                     /translation="MPSSIPYTHPSLVLGNIVNPDMLRRLRKISSLNDHIDAAQNRMN
                     AFITMKRSLEMTVSELLNMNVDVSAITAKIAAIDKQIAAAAANYITVRLDNESAIQTL
                     QEQIDEGQTDLALQSPVDFARSAIIKLPLAADSLHLDAQYFSYAENQETNPLGVVAEI
                     EDYLKAATAGLGTKAATNVSGRAVTQINLQRRNHQLSGTLIITATCTHKQARLFSPCI
                     LDPDRAVAIWNTCYPNDPIDPNKSTTSSGTPTSSANPTSGTPPSSAHTHTAAAPSSAT
                     PSSRIQPSSVNPTSGTPSTSAHSNSGTPPTSDTPNSSAHPNSSDPASNSSTSNTLSLL
                     SGATFGSCFIGMVHILKNEATGNNTALDTLANSLQDRFRIGNWVQEASGGFGVDASIA
                     EDIKSLLSTQTITTHVNLVTMGAIPSIRSNQLQIGVRSFRDVDPHRITTDLAALENLT
                     ATDKATIDRSAATAKTGARVMAMQGATIRTIMLGLGTIDHGANKTMDINTLMNAFEDY
                     IKEIKDGEASIPINFYVTTLSKTQLQNLTQPEPPASNPDSSSSKSNPPSPTPDTPTSN
                     PDPPSSPPSISSSTPDTQTSTPNISTSTSDTPTPDTPTSNPDSSSSTPDTPDVN"
     gene            complement(2933..4501)
                     /locus_tag="VKQ52_17370"
     CDS             complement(2933..4501)
                     /locus_tag="VKQ52_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89031.1"
                     /translation="MPSVIPYDPSLVLGNLVNQDALNNLVAISALEGPIDTAEDTLNS
                     FISTKRSLDMTIQELINMGIDTTDLIKQSTEVGKSIQTAAVDYAKAKVDGLTKIQPLK
                     AKIQTVHQEIESPIDYNKSQIKTMPLAADSLQMNVQYFAFDENTQDSNTQAATIKGFV
                     SNEVDFLGDSWKSQASAAAQSQINSQYERHSISGTLVISITCTHKDALLLAPFIIDVD
                     KAVRAWNRVYSSDMINPTDPASIAKIAAQANTQEEKSLTLLSGATYGSCFIGMVHVLN
                     TTTTTSSETMYSIAASIQEQFSIGGWFENLSGGFGVDTSFTNDAKNLLSTQNVTAHCT
                     LVTMGSIPSIKSNTVNFAVKSFANFDGAQAMSQLAALQNATEDAKTTVDSAATAARTG
                     QQMLAMQNSKIQGVLSGLNTIDNINNKILDINSMMDAMDDYIQKALAGNLGVPINYYL
                     KPITKSQLAEMWVAKYYPGRYLAISGDDSAPAGGTGNSGGNGGAGNAGSSGNSGGTGN
                     TGGSAFGNTATTAQ"
     gene            4720..5169
                     /locus_tag="VKQ52_17375"
     CDS             4720..5169
                     /locus_tag="VKQ52_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89032.1"
                     /translation="MDILPPDPLANSSIPLSTACERIANWQTAFGDPTTGCMVDPLLL
                     PRAAFIPIADIKALADKYEHFYGKPVVGVRAYFGLMHPKFEGHIRLLLVPVLAVYEPE
                     HPIQVHFRDLIVEHHRLSPDAGDETSIYDFTKPCPDFCDPTSILYNS"
BASE COUNT          891 a         1060 c         2013 g         1296 t
ORIGIN      
        1 ggctgtggcg aggaggtaat tcttgtgaag gcggatgttt tgggggttaa agattgtacg
       61 gagggtgttg tggagtttgg tgttgcggaa gggtttggag tttgggaaga gtttagtgtt
      121 gcggaagggt ttggtatttg ggacgatttt gccgttttcg aagagtttgg tgttttgaag
      181 ggctttgcta ttgtgtcggg ttttggtggt ggagcggggc gtattcaagt gccggttggt
      241 gcagccggta atcctgatgg cattcttata tatagtggcg tgttgatcga ggcggatgca
      301 gaggttttgg accagggcag cgatattttg ggcggcgaac cgggcctgga gggctgcggt
      361 ggagctgcgg tggataaaag ggctgccacc gttgttgcag ttgaggttga tgaccgggat
      421 catgatcttt tcgagcaggg gcgcgatgtc tttggcgccg ccatcctgga cccagttgcg
      481 aaagatgcgg aaatagcctt cgacccagat ccccgcggcg ggagacaggg tcttgccttt
      541 tacgaaggcg gcgagttcgt cgtgcagttg cacgagggtg tagaagttgt caaagaagtc
      601 cttacggtat tggttgtatt cggtgatggt gcggccgatc gtcgcggaga cgagctgatt
      661 ttcgcgatgg aggccgtaga aaagctcgcg ggtggggtca tggcgtgggg ccagccgatg
      721 gataagagtt gagaggttcc ggtcggggga caggcaggcg ttcaggcggg taagggatgg
      781 ggggattgcc gtggcgccgt cggggaagat cggtgggatc aggagatgaa tggcggtaag
      841 ggagttgagg ttgatcgaag agttgaggtt ggtcaaagag ttgctgttgg taagggcgcc
      901 gccgggaatg gagggctggg aaagggttgc gatcgttcgg cgcaggagca tatagtgatc
      961 gcggcaggcg gtggcgaatt ggcgtgcggc gttggcggct tccggggtga tgggtgtgca
     1021 tttcatagaa ggttagttta catccggggt gtcgggggtt gaggaggaag agtcgggatt
     1081 cgaagttggg gtatcggggg ttggggtatc ggatgttgag gttgagatgt tgggggttga
     1141 cgtttgggta tcgggtgttg aggatgagat gctggggggt gaggatggtg ggtcgggatt
     1201 cgaagttggg gtatcggggg ttggggatgg ggggttggat ttggaggacg aagagtccgg
     1261 gtttgaggcc gggggttcgg gttgggtgag gttttgcagt tgtgttttgg aaagggtcgt
     1321 gacgtagaag ttgatgggga tgctggcttc gccgtccttt atttccttta tatagtcttc
     1381 gaaggcgttc atgagggtgt tgatgtccat ggtcttgttg gcgccgtggt cgatcgttcc
     1441 cagcccaagc ataatggttc gaatggtggc gccctgcata gccatgacgc gggcgccggt
     1501 ctttgccgtg gcggcggacc gatcgatggt tgctttgtcg gtggctgtca ggttttccag
     1561 tgcggcgagg tcggtggtga tcctgtgcgg atcgacgtcc ctgaaggatc ttactcctat
     1621 ctggagttgg ttgctgcgga tggatgggat ggcgcccatg gtgacgaggt tgacgtgcgt
     1681 ggtgatggtc tgggtgctga gcaggctttt tatgtcttcg gcgatggagg catcgacgcc
     1741 gaagccgccg gaggcttcct ggacccagtt gccgatacgg aagcgatcct gcagactgtt
     1801 ggcgagggtg tcgagggcag tgttgttgcc ggtggcttcg ttcttcagga tgtgcaccat
     1861 gccgatgaag caggagccga aggtggcgcc ggagaggagg gagagggtgt tggaggtgga
     1921 ggagttggac gccgggtcgg aggagtttgg gtgggcggag gagttgggcg tgtcggaggt
     1981 tggcggggtg ccggagtttg agtgggcgga ggtcgagggg gtgccggagg tggggttgac
     2041 ggaggatggc tggatgcggg agcttggggt ggcggacgat ggagcggcgg cggtgtgggt
     2101 gtgggcggag gatggcgggg taccggaggt ggggttggcg gaagatgtcg gggtgccgga
     2161 ggaggtggtg gatttgttgg ggtcgatggg gtcgttgggg tagcaggtgt tccagatggc
     2221 gacggcgcgg tcggggtcga ggatgcaggg agagaagagg cgggcctgtt tgtgggtaca
     2281 ggtagccgtt atgatcagcg tgccggagag ctggtggttc ctgcgctgga ggttgatctg
     2341 ggtcacggcg cgaccactga cattagtggc ggccttcgtg ccgaggccgg cggtggctgc
     2401 cttgaggtag tcttcgatct ccgcgacgac acccaggggg tttgtctcct ggttctccgc
     2461 ataggagaag tattgcgcat cgaggtgcag ggagtccgcg gcgagcggca attttatgat
     2521 cgcgctgcga gcgaagtcga ccgggctttg gagggcgagg tcggtctgcc cttcgtcgat
     2581 ctgttcctgc agggtttgga tggccgattc gttgtcgagg cggacggtga tatagttcgc
     2641 ggcggcggcg gcgatctgtt tgtcgatggc ggcgatcttc gcggtgatgg ccgagacatc
     2701 cacgttcatg ttaagcaact cgctgaccgt catttccagg cttcttttca tcgtgatgaa
     2761 cgcattcatt ctgttctgcg cggcatcgat gtgatcgttc aggctgctga tcttccgcag
     2821 ccgccggagc atgtccgggt ttacgatgtt gcctaacacg agcgatggat gggtataggg
     2881 gatagaggaa ggcataacgg gggttcaggt agggtgatgt gcgatggttt agctattggg
     2941 cggttgtcgc cgtattaccg aaagccgagc cgccggtgtt gcctgtgccg ccggaattgc
     3001 ccgatgagcc agcgttgccg gcaccgccgt tgccgccgga gttgcccgta ccgcccgcgg
     3061 gggcgctgtc atcgccggag atggcgaggt agcgaccggg gtagtatttg gccacccaca
     3121 tttcggccaa ctgcgatttt gtgatgggtt tgaggtaata gttgatgggc acaccgaggt
     3181 tgccggcgag ggccttctgg atataatcgt ccatggcgtc catcatcgag ttgatgtcga
     3241 ggatcttgtt gttgatattg tcgatcgtat tgaggccgga gaggacgccc tggatcttgg
     3301 agttctgcat ggcgagcatc tgctgaccgg tgcgggcggc ggtggcggcc gagtccaccg
     3361 ttgtcttggc atcctcggtt gcgttctgca aggccgctaa ctgactcatg gcctgggcgc
     3421 cgtcgaagtt ggcgaaggat ttgaccgcga aattgaccgt attggatttt atcgagggaa
     3481 tggagcccat ggttacgagg gtgcagtggg cggtgacgtt ctgcgtgctg aggaggttct
     3541 tcgcgtcatt ggtgaagctg gtgtccacgc cgaagccgcc gctgaggttc tcgaaccatc
     3601 cgccgatgga gaattgctcc tggatgctgg cggcgatgga gtacatcgtt tcgctggaag
     3661 tcgttgtcgt ggtgttgagg acatgcacca tgccgatgaa gcaggagcca taggtcgcgc
     3721 cggagaggag ggtgagggat ttttcctcct gggtgttggc ctgggcggcg atcttcgcga
     3781 tgctggccgg gtcggtgggg ttgatcatgt cggaggaata gacgcgattc caggcgcgga
     3841 cggccttgtc gacgtcgata atgaagggag ccagcaggag ggcgtccttg tgggtgcagg
     3901 tgatgctgat gaccagggtg ccggagatac tgtggcgttc atattggctg ttgatctggg
     3961 actgggcggc tgccgaggcc tgggatttcc agctgtcgcc caggaagtcc acttcgttgc
     4021 tgacgaagcc tttgatggta gcggcctggg tgttggagtc ctgggtgttc tcgtcgaagg
     4081 cgaagtactg gacgttcatc tgcagtgaat ccgcggcgag gggcattgtc ttgatctgcg
     4141 acttgttgta gtcgatggga ctttcgatct cctggtggac ggtctgtatc ttggctttca
     4201 ggggctggat ctttgtcagt ccgtcaacct tggcctttgc gtagtcaacg gctgcggtct
     4261 ggatggattt accgacctcg gtggattgct ttatcagatc ggttgtgtct atgcccatgt
     4321 tgatgagctc ctggatggtc atgtcgaggg accgcttggt ggagataaag gagttgaggg
     4381 tgtcttccgc ggtgtcgatg ggcccttcca gggcgctgat ggcgacaagg ttgttcaggg
     4441 cgtcctggtt tacgaggtta ccgagtacca gggaggggtc ataaggaatt actgatggca
     4501 taaaaagggg atttttattg atgattaaag aaagttgccg ctattgggcg ccgctgtttg
     4561 gccggcagga caaagtaagc gtagtacgaa gggggatatc agcgcagaac ttcgcgttta
     4621 aaaaatcagg tgtttttacg gggtccgcgg atgagcggtg catgcaaaaa aaatctattt
     4681 tcaccgggca atccaaccat taaccttaat tcccgaacca tggacattct cccaccagat
     4741 ccgctagcga acagcagcat tcccctttct accgcatgtg agcgcattgc taattggcaa
     4801 acggcgttcg gcgacccgac gaccgggtgt atggtcgatc cgttattact gccgagggcg
     4861 gcttttatcc cgatagcgga tatcaaggcg ctggcagaca agtatgaaca tttttatggc
     4921 aagccggttg tcggagtgcg ggcctatttt gggcttatgc acccgaaatt cgaggggcat
     4981 atccgcctgt tgctggtgcc ggtgctggcg gtttatgagc cggagcatcc gatccaggtt
     5041 cattttcgcg acctgatcgt agaacatcac cgcttgtcac cggatgcggg cgacgagacg
     5101 tctatctatg attttacgaa gccctgtccg gacttctgcg accctacgag tattctgtat
     5161 aattcgtaag cgagggggtc gggtgtgagg agggcgtgcg cgaggggggc gtgtgcgatg
     5221 ggggcgtgag cgagcgggtt gtgggcgagc gggccgtgcg
//
LOCUS       DATGFG010000917         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1017552, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000917 DATGFG010000000
VERSION     DATGFG010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017552"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VKQ52_17380"
     CDS             complement(<1..1340)
                     /locus_tag="VKQ52_17380"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLZ89033.1"
                     /translation="MRRKPLLLSLLIPFIFIACQHKKAPGVKRPKLVVGIVVDQMRWD
                     YLYRYAAHYGEGGFRRLLKHGFSCDNTLINYLPAFTAVGHTCIFTGSVPAFSGIAGND
                     WVEQATGQRMYCTDDSTVKSVGAQDPGSKEGKMSPRNMLVSTVTDELMMAENFRSKVV
                     GVSLKDRAAILPAGHSATAAYWLEDSTGHFITSNYYMPKLPGWVDSFNKKEPVKEYVR
                     TKWTTLYPKNTYGESDTDDQPYEGKFKGDSTTAFPHNLDSSYKMQKSSFRSTPFGNTL
                     TLDFARAAVEGYQLGRGDVTDFLTINCASTDYVGHQFGINSIEEEDTYLRLDRDLASF
                     FTYLDETIGKEEYTVFLTADHGAAHAVWFSQNHDIPADFWLSAPLTDSLNAILGRTFQ
                     TANLVDTMSNYQVTFAMKTIRDKKLEFSAIKKVALDYLRQQPGITYAIDMANIG"
     gene            1442..>2200
                     /locus_tag="VKQ52_17385"
     CDS             1442..>2200
                     /locus_tag="VKQ52_17385"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HLZ89034.1"
                     /translation="MADIFEKLVKHNGPIGQHRERAHGYFAFPKLEGEIGSRMVFRGK
                     EKIVWSLNNYLGLANHPDIRKVDAEAAARFGMAAPMGARMMSGNSNYHEQLEKELAEF
                     EGKEDATLFNYGYQGIMSAIDAICGRHDVIVYDAESHACIIDGLRLHPGHRYVFKHND
                     VEDCEKQLQRATALIEKQGTGGILVITEGVFGMAGDQGKLKEIAALKDQYEFRLLVDD
                     AHGFGTLGKTGAGAGEEQGVQDKIDLYFSTFAKSM"
BASE COUNT          487 a          512 c          719 g          482 t
ORIGIN      
        1 tcgccgatat tggccatatc gatagcatag gtgatgccgg gctgctgccg gagatagtcg
       61 agggcaacct tcttgatcgc gctgaattcc agctttttat cccgaatagt cttcatggcg
      121 aaggtgacct ggtaattgga catggtgtcc acgaggttgg ctgtctggaa ggtccggccg
      181 aggatggcgt tgagactgtc ggtgagggga gcggaaagcc agaaatcggc ggggatgtcg
      241 tggttctggg aaaaccagac ggcatgggcg gcgccgtggt cggccgtcag gaagacggtg
      301 tactcctcct tgccgatggt ttcgtcgagg taggtgaaga aggaggcgag gtcgcggtcc
      361 aaccggaggt aggtatcctc ttcttcgatg gagttgatgc cgaactggtg accgacatag
      421 tcggtggaag cgcagttgat cgtcaggaaa tcggtgacat cgccccggcc caactggtag
      481 ccctccacgg cggccctggc gaagtcgagg gtgagggtat taccgaaggg agtggagcgg
      541 aaactggatt tttgcatctt ataggacgag tccaggttgt ggggaaaggc tgtggtactg
      601 tcgcctttga atttgccctc ataaggctgg tcgtcggtat cgctttcgcc ataggtgttc
      661 tttggataga gggttgtcca tttggtccgg acatattcct ttacgggttc ctttttattg
      721 aagctgtcca cccagcccgg caatttgggc atgtaataat tgctggtaat gaagtggccg
      781 gtggaatcct cgagccagta ggcggcggta gcgctatgac cggcggggag gatggccgcg
      841 cgatccttca gggagacgcc cacgaccttc gaccggaaat tctctgccat catcagttcg
      901 tcggtcaccg tggagacgag catgttgcgg ggggacattt tgccctcttt cgacccgggg
      961 tcctgggcgc cgacgctttt gacggtgctg tcgtcggtgc agtacattct ctggcccgtg
     1021 gcctgctcga cccagtcgtt gccggcgatg ccgctgaagg cgggtaccga gccggtaaag
     1081 atacaggtgt ggccgacggc ggtgaaggcc ggaaggtaat tgatcagggt gttgtcgcaa
     1141 ctgaagccat gtttcagcag ccgccggaag ccgccctcgc cgtaatgtgc ggcataacgg
     1201 tagagatagt cccagcgcat ctggtccacg acgataccga ccaccagttt cgggcgtttt
     1261 accccggggg cttttttgtg ctggcaggcg atgaaaataa aggggatgag aaggcttagc
     1321 aggaggggct tacggcgcat gggcaaaaat ttgcgcaaaa gtgcgggaaa attcgggcaa
     1381 aaccgcattg ccaaatgcgt tatttttgcg ggaccaacaa gcttaataat ttcataataa
     1441 tatggcagat atctttgaaa aattagtcaa gcacaacggc cctatcggcc aacaccggga
     1501 gcgggcacat ggatatttcg cttttccaaa actggaaggc gagatcggca gccggatggt
     1561 ctttaggggg aaagagaaga tagtttggag tcttaacaat tatcttggtt tggcgaacca
     1621 cccggatatc cggaaagtgg atgcggaagc ggcggcaaga tttgggatgg cggctccgat
     1681 gggggcgcgg atgatgagcg gcaacagcaa ttaccacgaa caactggaaa aagagctggc
     1741 cgaattcgaa ggaaaagagg atgctacgct cttcaactat ggttatcagg ggatcatgag
     1801 cgctatcgac gccatttgcg gccggcatga cgtgatcgtt tacgacgccg agagccatgc
     1861 ctgtatcatc gacgggctgc ggctgcaccc cggacaccgg tacgtattca agcataatga
     1921 tgtggaagac tgtgaaaagc agttgcagcg cgccacggcc ctgatcgaga agcagggcac
     1981 cggcgggatc ctggtgatta ccgaaggggt cttcgggatg gccggcgacc agggtaagtt
     2041 aaaagagatt gccgctctga aggatcaata cgagttccgt ttactggttg acgacgcgca
     2101 tgggtttgga acccttggca agacgggtgc cggcgccggc gaagagcagg gggtacagga
     2161 caagatcgat ctgtatttct ccacttttgc caagtcgatg
//
LOCUS       DATGFG010000918         8389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1419573, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000918 DATGFG010000000
VERSION     DATGFG010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419573"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VKQ52_17390"
     CDS             complement(<1..535)
                     /locus_tag="VKQ52_17390"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HLZ89035.1"
                     /translation="MEKRPVDVVVLSDVHLGTYGCRAKELVNYLKSITPNILILNGDI
                     IDGWQFNKRYFPVAHMMVIKEIIQFITNGTRVFYITGNHDEMMRRYTDIQMGNFALTD
                     KLVLEIDNKMTWIFHGDVFDNTTKGSAKILAKLGSSGYSLLIRFNRFINFILKSLGRE
                     RVSISKRVMAEVNKAVSK"
     gene            655..1443
                     /locus_tag="VKQ52_17395"
     CDS             655..1443
                     /locus_tag="VKQ52_17395"
                     /inference="COORDINATES: protein motif:HMM:NF025037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89036.1"
                     /translation="MKKWCFPVFAALTLSLTVRPIQSDAQALHYTVANTHSHNDYEQP
                     TPFWMAWQQQFGSIEADIWLVGGRVLIGHDREEIKMGRTLEEYYVKPLLACIEKYHGH
                     PYADTSRHLQILIDVKEDSVATLAALIALLDKYPALEHCSCLKWVISGNRPDPSLYVS
                     YPPFIAFDGILHAQYTPEELSRIAMMSDDLRYYTRWDSRAGIPDAGLDSLKQAIGHSH
                     AFHLPVRLWDAPDFPIAWRQLMQLQVDYLNTDHIADLAAYLHAL"
     gene            1510..2079
                     /locus_tag="VKQ52_17400"
     CDS             1510..2079
                     /locus_tag="VKQ52_17400"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HLZ89037.1"
                     /translation="MHEELILLIGDKIKTKRTEQNITLEQLAKKAGVSKGLISQIENN
                     RTVPSLPVLFNIIHSLEEDLRTFFEDMHDSFTNNHILIIRKGQEKLFSKEPVKGFSYK
                     RILTRSVSSQATDVVLLELKKNANRKQLIRTDAYECKYVLKGDVEYQVENELFTLHEG
                     DTLFFDGRAKHRLRNIGETEALILVFYLF"
     gene            2252..5560
                     /locus_tag="VKQ52_17405"
     CDS             2252..5560
                     /locus_tag="VKQ52_17405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89038.1"
                     /translation="MSKKITLVTGVLLVAFLLPLFSLAQKVITGHVLSKVDQSPVPGV
                     SVVIKGTRTGTSTSVDGSFAIKAKEGDVLVVSGVGVVRTEQEISGSDLTIAVAADARE
                     LNQVVVTATGIKKEAKRLGYALQTIDASTLTQAREADPVNALKGQAAGLEININSEIG
                     HPAGVIMRGENGANDQPIFVVDGVPISTDTYNLNADDIENFTVLKGPNAAALYGFQGK
                     NGAIIITTKKGAKAKGKPVVTFNTSDQINKGFIALPKYQDTYGPGDNGKYAFGGGGSS
                     ASSYFGSGAVGVGVNDYDYDVWGPQFRGQLLPQYDGAYTINQTYNTTFADGKTYTGHV
                     VPTPWVARGKDNLKKFIQAGELTANSISISSSTEKTDVRLSLGDTWQRGIVPNTQLNN
                     GTFTANVTERFNDKFSATAYFNYSRQSTPNVPDVNYGPNSIIYNIIIWGGADWSVNSP
                     NIRNYWQPGKVGIEQNYEEYYRYNNPWFMSYEWLRGHYQNNEYGYVSLNYKLNQNFDF
                     QFRPSFTAYDMFNSEKLPYSAGVYGRPLRQGDYREDRRQLFDNNEDLQVRYHKNQIAG
                     FLDLSAVAGGTVRNFSFNSSFESTNYLNAPGIYAFSNSQGALTGSNYASSMLVLSGYY
                     SVDLGYKSYITANVTGRVDKSSSLPTNSNSYYYPSFNLATVVSEYVHLPEAISFLKVR
                     ASYAESKSGGTYSTFSPNVSATPAGNYGYTWTSPYNGPGYQFTPTYTLTPTYTGQNSA
                     RYTDQVINPNINTADRKATEVGVDIRFLKNRIGLDVTRYRYWNQGIISQGTSNASGYA
                     TYLTNGDTYTNNGWEATISGKPVVNPHGLNWDVTANFFTFVKKWINYNSAPDNWEKNG
                     QRVDLVYGNAFIRTPDGKMVIDPASGEYITYSQNGSPAKKIFGHYDPDWQWGLINTFS
                     YKSFSMRFMFDGIVGGVMNDYVRQKTLQGGRHIESATGALGAARPSDEANIPAYTGDG
                     VNLTGPNGIQLDPISGKITNMKDLTQTTNTSKSLVQPFVYQASSISDLTTIKKTYVKL
                     REVTISYKVPQSTFGKKSIISNATVSLVGRNLLYFFPSRYKDLDVDQYTQDSGSGLQT
                     PTTRSYGININFSF"
     gene            5579..7279
                     /locus_tag="VKQ52_17410"
     CDS             5579..7279
                     /locus_tag="VKQ52_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ89039.1"
                     /translation="MKKTKYLFSLLMGVLLATSCSKKIADKEANPNNPSSVPPNLILG
                     TILTEMSGTSQDGSTGIGALGGVNSWDNVHDWNQYHCQNYNYYGNNIYSWTNGSTETE
                     NGIPNISDGPFNCYLVIKNVLKMDSEVTVRQGPAAALNPYEALGRFIKAYYFYNMTML
                     MGDVPLTQALQAPSNFTPSYTPQEQVFQYVLSQLDSANSDLATLISAQDNTLSASQDI
                     YYGGNLTQWQKAVNSFKLRVLVELSPKAADANLNVPAQFAAILGNPVKYPIFTSEADD
                     LAFVYNPGGSNTFSTYPFNPSNFGSIAGRFNMAATYVSALTTINDPRVFITCDPAWTL
                     FKNDSANPVQNPAQYQYFVGASTGLSLGTMYANASAGLYSFIGRKRYYSNFTGDPNVL
                     IGYKEMLFNIAEGIERGWATAGTAESWYKNGIIESMKFYGIDVTQTNFTAWFLPPTAN
                     AVTQVKPYPFTFSWATYYTQPGVQLSSNQATAIQQIVLQKYIAMFETSGYEPYYNWRR
                     TGVPVFQGGSGVGNNGVVPVRWAYPVTEQVQNTKNWQAALSNQGFTADDLNQKIWLLK
                     "
     gene            7294..8154
                     /locus_tag="VKQ52_17415"
     CDS             7294..8154
                     /locus_tag="VKQ52_17415"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HLZ89040.1"
                     /translation="MHTFSSYRLPAMAACIFHFAAGALLPLAAGAQRPLAPLPPAKHP
                     FIVVAHRGDHTQAPENTLAAYANAINAGADYVEIDLRTTADSQLVIMHDVSVDRMTNG
                     HGLVKDLAYDSLRRLKVSNKQHPEWGEFDIPTFSQVLELCKDKIYIYLDFKNADPAVA
                     YREISRYGMQRQVVVYINAPLQFSGWRNAAPAMPLMVSLPGNSRDTAAFNSFLDRYHP
                     EILDGRSDEYTEEMVRTATARGYLVLPDIQGAGEGPAVWEPAIRKEIRGLQTDHPGDL
                     IAWLKEKRLR"
BASE COUNT         2027 a         2419 c         2111 g         1832 t
ORIGIN      
        1 tcttgctgac ggccttattg acctccgcca taaccctttt tgagatggat acccgttccc
       61 ggcccaggga tttcagtata aagttaatga atctgttgaa ccggatcaag agggaatagc
      121 cgctgctgcc tagtttggcc aggattttgg cacttccttt ggtggtattg tcgaaaacat
      181 cgccgtggaa gatccaggtc atcttgttat cgatctccag gaccagctta tcggtaaggg
      241 cgaaattccc catctggata tccgtatacc ggcgcatcat ttcgtcatga ttgccggtga
      301 tatagaagac ccgggtccca ttggtaatga actggatgat ctccttgatc accatcatgt
      361 gggccaccgg gaaatagcgt ttgttaaact gccagccgtc gatgatatcc ccattcagga
      421 tcaatatgtt aggagtgatg ctttttaggt agttgacgag ttccttagcc cggcatccgt
      481 acgtcccgag atggacatcg gacagaacaa ctacatctac gggtcgtttt tccattattg
      541 tggtttccca aagttctaaa taagatatta ttgctatttt aagccagggt taacaaattg
      601 tgagtgttgt gttaatccag tgttaccatt gcaggataaa ttacctttgc gcccatgaaa
      661 aaatggtgtt tccctgtctt cgccgcactg acactatccc taacggtaag gcctattcag
      721 tcggacgcac aggccctgca ttataccgtc gccaacaccc attcccataa cgattacgag
      781 cagcccaccc ccttctggat ggcctggcaa cagcaattcg ggtccatcga ggcggatatc
      841 tggctggtcg gcggcagggt gctgatcggt cacgaccggg aagagatcaa gatgggacga
      901 acgctggagg agtattatgt caaaccgctg ctggcctgca tcgagaagta ccatgggcat
      961 ccttatgccg acacctcgcg gcatctccag atcctcatcg acgtaaagga ggattccgtc
     1021 gccacgcttg ccgccctgat cgcgctgctg gacaaatatc ccgccctcga acactgttcc
     1081 tgcctgaaat gggtgatcag cggcaaccgg ccggaccctt cgctctatgt ctcctatccc
     1141 cccttcatcg ctttcgacgg catcctgcat gcacagtaca ccccggagga gctttcccgg
     1201 atcgcgatga tgagcgacga cctgcgctac tacacgcgct gggattcgcg cgcgggtatc
     1261 ccggacgccg gtctcgacag ccttaaacag gccatcggcc acagccatgc cttccatctt
     1321 cccgtccgcc tctgggatgc gcccgatttt cccatcgcct ggcgtcagct gatgcaattg
     1381 caggtggatt atctcaacac ggaccatatc gccgaccttg cggcctacct ccatgcgctg
     1441 tgagcagccg accccgattt tcaccggatg cctaatttgc ttatcttttc gggcaaacag
     1501 ccaagaggta tgcacgaaga actgatcctg ctcatcgggg ataagatcaa aaccaagcgg
     1561 acggagcaga atatcacact ggaacaactg gctaaaaagg ccggtgtcag caagggcctg
     1621 atctcccaga tcgagaacaa tcgtacggtg ccctcccttc ccgtgttgtt caatatcatc
     1681 cattccctcg aagaggacct ccgcaccttc ttcgaagaca tgcacgacag cttcaccaac
     1741 aatcacatcc tcatcatccg gaaaggccag gagaagctct tttcaaagga gcccgtgaaa
     1801 ggcttctcct acaaacgcat cctcacccgg agcgtcagtt cgcaggccac cgatgtcgtc
     1861 ctgctcgaac tgaagaagaa cgccaaccgc aaacagctta tccgcaccga cgcctacgaa
     1921 tgcaaatacg tcctcaaagg cgatgtcgaa taccaggtgg agaacgagct cttcacgctt
     1981 catgaaggcg acacgctttt cttcgacggc cgcgccaaac accgcctcag gaatatcggc
     2041 gagaccgagg ctctcatcct cgtattctat ctattctgaa taaagggaaa caccggcctt
     2101 ttcataattt tacaaatact taacattggt ttagtatttg taaactgtta acatttactt
     2161 aaatcagccc taacctttcc gtcacattga ccgtatactt tcgccgttga gatatatacg
     2221 gcttcatttt caactaaaaa catactgtat tatgagtaag aaaattaccc tcgtgacggg
     2281 tgtcttgctt gtggcattcc tgttgccact tttttccctc gcacaaaagg tcatcaccgg
     2341 gcatgttctc tccaaggtgg accagtcgcc ggttcccggc gtttcggtgg taatcaaagg
     2401 aacaaggacc ggcacatcca cttctgttga cggcagtttc gccatcaagg cgaaggaagg
     2461 ggatgtcctg gtggtttcgg gtgtaggcgt ggtgcgcacc gaacaggaga tcagcgggag
     2521 cgatctgacg attgccgtgg cggcggatgc cagggaactg aaccaggtgg tggtaacggc
     2581 caccgggatc aagaaggaag cgaagcgcct gggctatgcc ctgcagacca tcgacgcttc
     2641 cacgctgacc caggcgcgcg aggccgatcc cgtcaacgcc ctgaagggcc aggccgctgg
     2701 tctcgagatc aacatcaatt cggagatcgg tcaccctgcc ggggtgatca tgcgcgggga
     2761 gaatggcgcc aatgaccagc ccatcttcgt cgtggatggg gtacctatca gcaccgatac
     2821 ctataacctc aacgccgacg atatcgagaa ctttaccgtg ctgaaaggac ccaatgccgc
     2881 ggcgctctat ggtttccagg gtaagaacgg cgccatcatc atcactacga agaaaggcgc
     2941 aaaggccaag ggcaagccgg ttgtcacctt caataccagc gaccagatca acaagggctt
     3001 tatcgccctg cccaaatacc aggataccta tggtcccggc gacaacggta agtatgcctt
     3061 tggcggcggc ggttccagcg cttcctctta ttttggttcc ggcgccgtcg gtgtcggggt
     3121 caatgactat gactacgacg tttggggacc tcaattcagg ggccagttgc tgccgcagta
     3181 cgacggggcg tatacgatca accaaacata taacacgacc tttgccgatg gtaaaaccta
     3241 taccggccac gtagtgccta ctccctgggt agcccggggc aaggataacc tgaagaaatt
     3301 catccaggcg ggtgaactca cggccaacag catttccatc agcagctcta cggaaaagac
     3361 ggatgtccgg ctctccctcg gcgacacctg gcagcggggt atcgttccca acacccagct
     3421 caacaacggg acgttcacgg ccaatgtcac ggaacgcttt aacgataaat tcagcgcgac
     3481 cgcttatttc aactacagcc ggcagtccac tcccaatgta ccggatgtga attatggtcc
     3541 caacagcatt atttacaata tcatcatctg gggcggcgcc gactggagcg tgaacagccc
     3601 caatatccgc aattactggc agcccggaaa agtgggcatc gaacagaact atgaagaata
     3661 ctaccggtat aacaacccct ggttcatgtc ttacgaatgg ctgcggggtc actaccagaa
     3721 caatgaatat ggctatgtat ccctcaacta taagctgaac cagaacttcg atttccagtt
     3781 ccgccccagc ttcacggcct atgatatgtt caactcggag aagctgccgt attccgccgg
     3841 ggtttatggc cggccgctcc ggcaggggga ttaccgcgag gacaggagac agctgttcga
     3901 caacaacgaa gatctgcagg ttcggtatca taagaaccag atagccggct tcctcgatct
     3961 ttcggcagta gcaggcggca cggtgcgtaa tttcagcttc aattccagct tcgaatccac
     4021 caactacctg aatgccccgg ggatctatgc tttctccaac tcccagggcg ctttgaccgg
     4081 aagcaattat gcttcttcga tgctggtgct tagcggctat tattccgtcg accttggata
     4141 caaatcgtat ataacggcga atgtcaccgg tcgcgtggat aaatcttctt ccctgcccac
     4201 caatagcaat agctactatt acccctcctt caacctggcg accgtggtct ctgaatatgt
     4261 acacctgccg gaagccatct ccttcctcaa ggtgcgggcc tcctatgcgg aaagcaagag
     4321 cggcggcacc tactccacct tctcgccgaa tgtaagcgcc accccggccg gcaactatgg
     4381 ctatacctgg acctctcctt acaacggacc cggttaccag ttcacgccca cctatacgct
     4441 cacgccaacg tataccggcc agaacagcgc ccggtacacg gaccaggtga tcaaccccaa
     4501 catcaacacg gcggacagaa aagccactga ggtgggtgtg gacatccgtt tcctgaagaa
     4561 caggatcggc ctcgacgtta cccgatacag gtattggaat caggggatca tcagccaggg
     4621 cacttcgaac gcgtccggct atgccacgta tcttaccaat ggggacacgt ataccaataa
     4681 tggctgggaa gccacgatca gcggtaaacc cgtcgtcaac ccgcatgggc tcaactggga
     4741 tgtaacggcc aacttcttta cgtttgtaaa gaaatggata aactacaact cggcgccgga
     4801 caattgggag aagaacggtc agcgcgtcga cctggtatat ggcaacgcct ttatccggac
     4861 tcccgacggt aaaatggtga tcgatcccgc ttccggtgaa tacatcacct actcgcagaa
     4921 cggctctccc gccaaaaaga tattcgggca ttatgatccg gactggcaat gggggttgat
     4981 caacaccttc agctataaga gtttctcgat gcgctttatg ttcgacggta tcgtcggcgg
     5041 ggtgatgaac gactatgtcc ggcaaaaaac gctgcagggc ggccgtcata tcgaatccgc
     5101 taccggcgca ttgggtgcgg caaggccttc cgacgaagcc aatattcccg cctatacggg
     5161 cgatggggtc aacctgaccg gtccgaatgg cattcaactg gatcccatca gcgggaaaat
     5221 taccaatatg aaggacctga cccaaacgac gaatacgagc aagagccttg ttcagccctt
     5281 cgtctaccag gcatcttcca tttccgacct gacgacgatc aagaagacct atgtcaagtt
     5341 gcgggaagta acgatctcgt acaaggttcc gcaaagcact ttcggcaaaa agtcgatcat
     5401 cagcaacgcc actgtctcgc tggtcggccg gaacctgctc tatttctttc ccagccggta
     5461 caaggacctg gacgtagacc aatacaccca ggattcgggt tcgggcctgc aaacgccgac
     5521 gacgcgcagc tatgggatca acatcaattt ttctttctaa ctacataaga aaaagaatat
     5581 gaaaaagaca aaatatctat tttcccttct gatgggcgtg ttgctggcga cctcctgcag
     5641 caagaaaata gcggataaag aagccaatcc caataaccct tccagcgtcc cgccgaacct
     5701 gatcctgggt acgatcttaa cggaaatgtc cggcacgtcg caggacggca gcaccggcat
     5761 cggcgccctg ggcggggtca actcctggga caacgtgcat gactggaacc aataccattg
     5821 tcagaattat aactattacg gcaataacat ctattcctgg acgaatggtt ccactgaaac
     5881 ggagaacggt atcccgaaca tcagcgatgg accgttcaat tgctacctgg tgatcaagaa
     5941 tgtactcaaa atggactcgg aggtgacggt caggcaaggt cccgccgcgg cgctcaatcc
     6001 ctatgaggcc ctcggcaggt tcatcaaggc ttattatttc tataacatga ccatgctgat
     6061 gggtgatgtt cctctgaccc aggccttgca ggcgccgtcg aacttcactc cgtcgtatac
     6121 gccgcaggag caggtgttcc aatatgtatt gagtcagctg gattcggcca atagcgacct
     6181 ggccaccctc atttccgcac aggacaatac cctgtcggct tcccaggaca tctattatgg
     6241 ggggaatctc acccaatggc agaaagcggt caactccttc aagttgcggg tattggtaga
     6301 gcttagcccg aaggctgccg atgccaacct gaatgttccg gcgcaattcg ccgctattct
     6361 cggcaacccc gtcaaatatc ccatcttcac cagcgaagcc gatgatctgg cctttgtcta
     6421 taaccccggc gggtcgaata cctttagcac ctatcctttc aatcccagca acttcggctc
     6481 tatcgccggc cggttcaata tggcggctac ctatgtcagc gcgctgacca cgatcaacga
     6541 cccccgtgta ttcatcacct gcgaccccgc ctggacgctg tttaaaaatg attcggcgaa
     6601 cccggttcaa aacccggccc aataccaata ttttgtcggt gccagcaccg ggctttcgct
     6661 tggaaccatg tatgccaatg ccagtgcggg cctgtattct tttatcggcc ggaaaaggta
     6721 ctattccaac tttacgggtg acccgaatgt cctgatcggg tacaaggaaa tgcttttcaa
     6781 catcgccgaa ggcatcgaac gtggctgggc taccgcaggt acggcggagt cctggtacaa
     6841 gaacgggatc atcgaatcca tgaaattcta tggcatcgat gtgacccaga ccaatttcac
     6901 cgcctggttc ctgccgccga cggccaacgc cgtcacgcag gtgaagcctt acccgtttac
     6961 cttcagctgg gccacctatt atacgcagcc gggtgtgcaa ctttcttcca accaggcgac
     7021 ggccattcag cagatcgtcc tgcagaagta tatcgccatg ttcgagacct cggggtatga
     7081 gccctactac aactggcgcc ggacgggtgt tcccgtcttc cagggcggca gcggcgtagg
     7141 taataacggc gtcgtcccgg taagatgggc ctacccggtt accgagcagg tgcagaatac
     7201 caagaattgg caggcggcct tgtccaacca gggcttcacc gccgatgacc tgaaccagaa
     7261 gatctggttg cttaaatgat aagcgttcaa cttatgcata ctttttcttc ctacaggctg
     7321 ccagcgatgg cagcctgtat ttttcatttt gcggccggcg ccctgcttcc gctcgcagcc
     7381 ggcgcccagc gccccctggc tcccctcccg ccggcaaaac accctttcat cgtggtggcc
     7441 cacaggggag atcatacgca ggcgccggaa aatacccttg ccgcctatgc caatgccatc
     7501 aatgccggcg cggattatgt cgagatcgat ctccgcacca ccgcggacag ccagctcgtc
     7561 attatgcacg acgtctcggt cgaccgcatg accaacggcc atgggctggt aaaggacctc
     7621 gcctatgatt cgcttcgccg cctgaaagta agcaacaaac aacatcccga atggggggaa
     7681 ttcgacatcc ccaccttcag ccaggtgctg gaattgtgca aggataagat ctacatctac
     7741 ctggacttca agaatgccga ccccgccgtg gcctataggg agatatcccg ctatggaatg
     7801 cagcggcagg tggtcgtgta tatcaacgcg ccccttcaat tctccggctg gcgcaacgcc
     7861 gcacccgcca tgcccctcat ggtcagcctt cccggaaata gcagggatac agccgcattc
     7921 aacagcttcc tggatagata ccatcccgag atcctggacg gcagatccga tgaatacacg
     7981 gaagaaatgg tgcggacagc gacagcacgg ggctatcttg tcctccccga catccagggc
     8041 gccggtgaag gccctgcggt atgggagccg gccatccgga aagagataag gggactgcag
     8101 accgatcacc ccggcgacct catcgcctgg ctgaaggaaa aacggctgcg ttagccgcgg
     8161 ctaagtttta aacattattt aatagggcgc tcgcggtggc tagccccatt ttcgatactt
     8221 tgtagatcat taatcacaaa actcgtttac tatgaccaat gagcaagccg aaaagatcac
     8281 gaacgagatc atggaccttt atgaagggta tggcggggct gaatatgcgg gtgaaaaggt
     8341 atcccagttg gaacacatgg cccaggcggc tcagttggct gaggaacag
//
LOCUS       DATGFG010000919         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1535331, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000919 DATGFG010000000
VERSION     DATGFG010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535331"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VKQ52_17420"
     CDS             complement(<1..1066)
                     /locus_tag="VKQ52_17420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HLZ89041.1"
                     /translation="MSLVDLVMPKMGESIMEATILKWHKQPGDAVKQDETVLEIATDK
                     VDTEVPSITEGIVEEVLYKVNDVVPIGSVIARIRTSNGTAASAPVQAAVTTTPNPVAI
                     PVPPVSQHHHPAPAAVAANPGGPRFYSPLVLNIAANEGVPLSELESIPGTGNEGRVTK
                     KDILQYIADRRPGHLPAATVTGYATAAQPITGYSTSTPPPSVTPVRAESPIRATPESI
                     SSQPPMTIQSGNVEIIEMDRMRRLIADHMVRSIHTSAHVTSISEADVTHLVHWREKVK
                     KEFEKRENTKLTFTPLFIEAVIRCIKKYPLINSSVDGDKIIVKKDINIGMATALPSGN
                     LIVPVIRNADQLNLTGLARQV"
     gene            complement(1103..>2186)
                     /locus_tag="VKQ52_17425"
     CDS             complement(1103..>2186)
                     /locus_tag="VKQ52_17425"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HLZ89042.1"
                     /translation="ITGGLGPTADDITKPLLRDYFGGNMVVDEKALDNVKWIFERMLK
                     RPMIERNLQQAEVPDSCTVIQNKRGTAPGMWFEKDGRIFVSMPGVPHEMKGMMTDDVL
                     PALRRRFQLPYIAHRTLLTAGVGESFLAEHIKTFEEALPASLKLAYLPNYGMVRLRLT
                     VRGEDPTGLETLLQEQFDTLKGLVTEWMVTDEDIPIEAAVGRLLLAGGHTLGTAESCT
                     GGYIAHLITSISGSSRYYKGSVVSYANEVKTGLLGVNPDTLARNGAVSEATVQEMVRG
                     ALSRLDTDFAIATSGIMGPDGGTPEKPVGTVWVAVGNRQNILTQKFSFRFDRQRNIEL
                     TATNALNLLRKFILAAASKPSPVIRS"
BASE COUNT          369 a          530 c          753 g          534 t
ORIGIN      
        1 ttacctggcg ggccagcccg gtgaggttca gctgatcggc attacggata acggggacga
       61 tgaggttacc ggagggcagg gcggtggcca tgccgatatt gatatccttt ttgacgatga
      121 tcttgtcgcc gtcgaccgaa ctgttgatga gcggatattt ctttatgcag cggatgaccg
      181 cttcgatgaa caggggggta aaggtgagtt ttgtattctc ccttttttcg aattcctttt
      241 tcactttttc gcgccagtgg acgagatggg tgacgtcggc ttcggagatg ctggtgacgt
      301 gggcgctggt gtgtatgctg cggaccatat ggtcggcgat gagcctgcgc atccggtcca
      361 tttcgatgat ctcgacgttg ccgctttgga tggtcatggg cggctgggag gagatgcttt
      421 ccggggtggc acggatgggg ctttccgcgc gtacaggggt gacggaaggc ggcggggtgg
      481 aggtggagta gccggtgatg ggctgggcag cggtagcgta accggtgacg gttgcggcgg
      541 gcagatggcc gggtctccgg tcggctatat attgaaggat atcctttttg gtgaccctgc
      601 cttcattgcc ggtgccgggg atcgattcca gttcgctgag tggaacgccc tcgttggctg
      661 cgatgttgag tacgaggggg gaatagaacc gtgggccgcc ggggttggcg gcgacggcag
      721 cgggggcggg atggtgatgt tggcttacgg gcgggaccgg tatggcgacc gggtttggtg
      781 tcgtggtaac ggcggcctgt acaggagcgg aggcggcggt gccgttgctg gtccggatac
      841 gggcgatgac cgagccgatg ggtacgacat cgttcacctt atatagtacc tcttcgacga
      901 tgccttccgt gatggaagga acctcggtat cgactttgtc ggtggctatt tccaatacgg
      961 tctcatcttg ctttacggca tcgccgggct gtttgtgcca cttcaggatg gtggcttcca
     1021 tgatactttc acccattttc ggcattacca ggtcaacaag agacataata tggcattttt
     1081 aaggtagcaa aggtaatgtt ttttagcttc ttatgaccgg gctgggtttg gaggcagcgg
     1141 ccaggatgaa tttccttaac agattcaggg catttgttgc cgttaattca atattccgtt
     1201 gccggtcaaa gcggaacgaa aatttttgcg tgagaatatt ttgacggttt ccaactgcca
     1261 cccagaccgt tcctaccggt ttttcgggtg tgccgccgtc ggggcccatg atcccggagg
     1321 tagcgatcgc gaaatcggtg tccagccggg acagtgcgcc ccggaccatt tcctgtaccg
     1381 tcgcttcgct gacggcgccg ttgcgggcga gggtatcggg gttcacgccg agtagcccgg
     1441 tctttacttc gttggcatag ctgacgacgc ttcctttgta ataccggctg ctgccggaga
     1501 tggaggtgat gaggtgggcg atatagccgc cggtgcaact ctcggccgtg ccgagggtat
     1561 ggccgcccgc aaggaggagg cggccgacgg ccgcttcgat ggggatgtcc tcgtcggtga
     1621 ccatccactc ggtgaccagt cctttcaggg tgtcgaactg ttcctgcagg agggtttcca
     1681 gccctgtggg atcctctccc ctgacggtga ggcggaggcg taccattcca tagttgggga
     1741 ggtaggccag tttgagggag gcgggcagcg cttcttcgaa tgtctttatg tgttcggcga
     1801 ggaaggattc tccgacgccg gcggtgagca gggtgcgatg ggcgatatag ggaagctgga
     1861 agcgccggcg cagggcgggc agcacgtcgt cggtcatcat ccctttcatt tcatggggga
     1921 caccgggcat cgagacgaag atgcggccgt ccttctcgaa ccacatcccg ggggccgtgc
     1981 cgcgtttgtt ctggataacg gtgcaggagt cgggcacctc ggcctgctgg aggttgcgtt
     2041 cgatcatcgg ccgtttaagc atgcgttcga agatccattt tacgttgtcc agggcttttt
     2101 cgtcgacgac catattgccg ccaaaatagt ccctgagcag cggttttgtg atgtcatcgg
     2161 cggtgggccc caggccgccg gtgatg
//
LOCUS       DATGFG010000920         5553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_508162, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000920 DATGFG010000000
VERSION     DATGFG010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508162"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VKQ52_17430"
     CDS             complement(<1..889)
                     /locus_tag="VKQ52_17430"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ89043.1"
                     /translation="MLKRFIERPVLSTVVSIILLILGGLSIFTLPISLFPDIAPPSVQ
                     VQATYPGANSEVVARSVATPLEEAINGVEGMTYMTSNSSNDGSMLLTVFFKQGTNPDI
                     AAVNVQNRVSKATSQIPAEVIQAGISTQKQQNSIIMFIGLYSTDSSYDETFLQNYAKI
                     NLIPKIQRIPGVADAQPFGTKDYSMRIWLKPDRLVANNLSPVEVTNAIKDQSLEAAPG
                     RLGQSSKEVYEYVLKYKGKLNKNEDYENIIVKSNSDGSVVRLGDVARVEFGSYTYSSN
                     STLNGHPTTGIAIQQLSGSN"
     gene            complement(901..2055)
                     /locus_tag="VKQ52_17435"
     CDS             complement(901..2055)
                     /locus_tag="VKQ52_17435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ89044.1"
                     /translation="MRKYLVFSVILIAAATYFSGCNQAQGTQAAPPPQTLPVLPINTS
                     AATTYLTYSASVEGKTNVEVRPQVSGYLDKIYVEEGAYVTAGQPLFKINDRPYDAQLH
                     NSEANIEAAKANMEKAAIEVHRLQPLVENKVIADVQLKAAQAAYDAAKAQVTQAEAES
                     NNAGINLGYTLLKAPVSGYIGRIPFKVGALVGKGEAQPLTVVSDVKEVYAYFGMSESD
                     FLKFTSQVPGKTINEKIKGLPPIELQLADKSIYANKGRIELMEGQFDRNIGAITFRAT
                     FPNAAGLLRSGITGEVRIPQSSSGLLPVPQTSTYELQDRIFVFTVGDSNKVVSKQLHI
                     VAKTTNYYLVDKGVQAGDKIVFAGMDRLQDGAVINPQQLSADSVLKVMPL"
     gene            complement(2264..2893)
                     /locus_tag="VKQ52_17440"
     CDS             complement(2264..2893)
                     /locus_tag="VKQ52_17440"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ89045.1"
                     /translation="MGRPQARRNHMGSKERIHRLKEQTRVNILDAAHDIVKDEGWQAL
                     SMRKIADKIEYTAPIIYEYYANKEAILLELTKKGFMLLACRMNEAKNRHTSLEDQLEA
                     MWLAYWNFAFEEKEYYQLMFGVEVNCCIMDEILPPGSTPYDIAAGPIAELMGISDPDD
                     DRVCTKYYTYWSIIHGLISINIVRKGTSDEINRQILKDAITGITRSLKN"
     gene            complement(3000..5384)
                     /locus_tag="VKQ52_17445"
     CDS             complement(3000..5384)
                     /locus_tag="VKQ52_17445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89046.1"
                     /translation="MLRNYLRIAFRNLWRHKGFSLLNILGLTVGMVAFFLIFLYVRFE
                     LSYDSMYTKADRVYRIVCDIKTPTETIHASQPSWAVPPHLTAELPEVEAALRLGMGDN
                     WLVYRGDQQFAQDDVATADSTFFQVFDLPLLRGDRKTALKEPFSVVLTEKTAKTFFGN
                     ADPMGQSLILTRQKFHATVTGVMKDIPDNSTIRASMVVSMNTITRRLNPGIDDQWGNY
                     GPNGYVLLRPGASAAALEKKFPAFLEKMDGREMRESKMVPTLYLERLRDVYLRSTRDG
                     SKTGNITDVYIFSIIGVFILLIACINFINLTTARSTDRAKEVGIRKVAGAVKGQLARQ
                     FIGESIILCLIAFLMAVILTALLIHPFNQIAGKIVSPGIFSQGSDLLILLGIAIGIGI
                     AAGIYPALVLSSFQPVEVLKGRFATGTRGIFLRKALVIVQFTIATGLIIATLVVYNQL
                     HYMRSQDLGFNKDQKMILDTRGDSARLAVKQVVSGLPGVLSTAMAGSVPGGGNPGAYS
                     QIENTRGEMQIANLDLYFVDFDYIPQFKMKIVAGRAFSRDFMTDTTNAMILNEAAVKL
                     FGYRSPQEAVGRKFDQWGRKGTIIGVVKDFHFRSLQEVIKPLSMRIEPRACDLLSVNV
                     DARHLPATIAGIERTWKRILPDKPFSYFFLDEYFDKQYRAEDRFGRLFLYFAVLAIVI
                     SCLGLMGLASYSTLQRTKEIGIRKIVGASVGNIVFLLSRDFLILVAWSFLVATPLSWY
                     FLNGWLRGFAYRIDVYWWIFAAAGFTALLVALLTISFQSIKAAVVSPVKSLHAE"
BASE COUNT         1206 a         1446 c         1546 g         1355 t
ORIGIN      
        1 cattggagcc ggacagctgt tggatcgcaa tacccgtggt agggtgaccg ttcagcgtac
       61 tgttcgaaga gtaggtatac gagccgaatt cgacccgggc cacatccccc aaccggacca
      121 cggaaccatc gctgttggac ttgacgatga tattctcata gtcttcgttc ttgttcagtt
      181 tgcccttgta cttcaacacg tattcataca cttccttgct gctctgaccg aggcggccgg
      241 gagccgcttc caggctttga tccttgatgg cattggtcac ttccaccggg gaaaggttat
      301 tggcaaccag ccggtctggt ttcagccaga tgcgcatgga ataatccttg gtgccgaaag
      361 gctgggcatc cgcgacgccg ggtatgcgct ggatcttggg gatcaggttg atcttcgcgt
      421 agttttgcaa gaaagtctca tcatagctgc tgtcggtgga ataaagaccg atgaacatga
      481 tgatgctgtt ctgttgtttt tgcgtggaga tacctgcctg gataacttcc gccggtatct
      541 ggcttgtggc cttggacacg cggttctgta cgttcaccgc tgcgatatcg gggttagtac
      601 cctgtttgaa gaagacggtc agcagcatgc ttccgtcgtt gctggagttg gaggtcatat
      661 atgtcatgcc ctccacacca ttgatggctt cttccagcgg agtggcgacg ctgcgcgcaa
      721 cgacttcgga gttggcgccg ggataggtgg cctgtacctg tacgctgggt ggcgcaatat
      781 ccgggaagag cgaaatgggt agtgtaaaaa tggataaacc acccagtata agcaaaatta
      841 tagacacgac cgttgataac accggccttt caataaatct ctttagcata attttaattt
      901 ttagagcggc atcactttga gtacgctgtc tgcggacagt tgctgcgggt tgatcaccgc
      961 gccgtcctgc aggcggtcca tacctgcgaa cacgatcttg tcgccggcct gtacaccttt
     1021 atctaccaaa taataattgg ttgtttttgc aacgatgtgc aattgtttgc tgacgacctt
     1081 attgctatcg ccaacggtga acacaaagat cctgtcctgt aattcatacg tggatgtctg
     1141 gggtacgggc aaaagaccgg agctgctctg cgggatgcgc acttcgcctg tgatgccgga
     1201 gcgtaacagc cctgctgcat tggggaaggt ggcccggaac gttatcgccc cgatgttcct
     1261 gtcaaactgc ccttccatta actcgatcct gcctttgttg gcataaatgc tcttgtcggc
     1321 cagttgcaat tcgatgggtg gtaatccctt gatcttttcg ttgatcgttt tgccgggaac
     1381 ctggctggtg aattttagaa agtcgctctc gctcattcca aaataagcat atacctcttt
     1441 cacatcggag accaccgtca gcggttgcgc ctcacccttg cccacaaggg cgccgacttt
     1501 gaaggggatg cggccgatat aaccgctcac gggggctttc agcagggtat aaccgaggtt
     1561 gatcccggcg ttattgcttt ctgcttcggc ctgggttacc tgtgcctttg ccgcatcgta
     1621 agcggcctgg gcggccttca gctgaacatc ggcaatgacc ttgttctcta ccagcggctg
     1681 gaggcggtga acttcgatcg cggccttctc catattggct ttggcggctt cgatgttggc
     1741 ctcgctgttg tgcagctggg cgtcataggg acgatcgttg atcttgaata aaggctgacc
     1801 ggcggtgaca taggcgcctt cctcgacata gatcttgtcg agataaccgc tcacctgggg
     1861 gcggacctcg acattggtct taccttccac cgaagccgag taggtcagat acgtcgttgc
     1921 tgcggaagta ttgatcggga gcaccggtaa tgtctgcgga gggggtgcgg cctgcgtacc
     1981 ctgagcctgg ttacagccgc tgaaataagt tgcagcggct atcaaaatga cggaaaaaac
     2041 tagatacttt ctcataacct tattaaattg tttaatggtg ttaaatcaaa cgctaggaaa
     2101 ggacttttgt tcatgatatt taccagtgtt aaataaaaaa ggaaaaaaat ttaccccttc
     2161 atacagcaat gacgagcgga gttctgctaa gaagacagtt gacccgatct tttttggaca
     2221 atcgtagaaa agtttttttt ggcacggtgc caggccgggg agattagttc ttcaggctac
     2281 gggtgatgcc ggttatggca tccttcaaaa tctgccggtt tatctcgtcc gaagtacctt
     2341 tccggacgat gtttatggag atgaggccgt ggatgatcga ccagtacgta taatatttgg
     2401 tacataccct gtcgtcatcg gggtcgctga tacccattag ttcggcaata gggccggcgg
     2461 cgatatcata aggtgtggat cccggtggaa gtatctcatc catgatacaa cagtttacct
     2521 cgacaccgaa catcaattga taatattctt tttcctcgaa ggcgaaattc cagtaagcaa
     2581 gccacatggc ttccagctga tcttcgaggc tggtatgcct gttcttcgcc tcgttcatcc
     2641 ggcaggccag cagcataaac cctttcttgg tcagttccag gaggatggcc tccttgttgg
     2701 cgtagtattc gtagatgatc ggagcggtgt actctatctt gtctgcgatc ttgcgcatac
     2761 tgagcgcctg ccagccttca tctttcacga tgtcgtgtgc cgcatccagg atatttaccc
     2821 tggtctgctc ttttaatcga tgaattctct ccttgctccc catgtggttt cttctcgcct
     2881 gcgggcgccc cacctcccga tgggttttac tctcgtaaat aatttaacac tgtaaaatta
     2941 gtaagatttt ccggacacca acaaattttt ttttcaccgg cgttccgggc ggggccggct
     3001 tattcggcgt gcaaggactt caccgggctt acgacagccg ccttaatcga ttgaaaacta
     3061 atagttagca aagcgacgag aagggcggtg aaacccgccg ccgcaaagat ccaccaatat
     3121 acatcgatcc ggtaggcgaa tccccgcagc cagccgttga ggaaatacca ggacaggggg
     3181 gtggcgacca ggaacgacca ggccaccagt atcaggaagt ccctggacag caggaacacg
     3241 atattgccta cggaggcgcc cacgatcttg cggataccga tctcctttgt ccgctgcagt
     3301 gtgctgtagg aagccaggcc catcaaccca aggcagctga taacgatggc gagcacggca
     3361 aaatagagga agagtcttcc gaagcggtct tcggccctgt actgtttgtc gaaatattca
     3421 tcgaggaaga aatagctgaa gggtttatcg ggcaggatcc gtttccaggt gcgctcgatg
     3481 ccggcgatgg tagccggcag atggcgtgcg tcgacattga cggacaagag atcacaggcc
     3541 cgtggttcga tccgcatgct aagcggtttg atcacttcct gcaggctgcg gaaatgaaaa
     3601 tcttttacca ccccgatgat cgttcccttt ctgccccact ggtcgaactt ccggccgacg
     3661 gcttcctgcg gggaccggta cccgaacagt tttaccgcgg cctcgttcag gatcatggcg
     3721 ttggtggtat ccgtcataaa atcccgcgag aaggcgcggc ccgcaacgat cttcattttg
     3781 aattgcggga tataatcgaa gtccacgaag taaagatcga ggttggcgat ctgcatttct
     3841 ccgcgggtat tttcgatctg cgaataggcg ccggggttgc cgccgcccgg aacgctgcct
     3901 gccatggccg tggagagcac acccgggagc ccggagacca cctgttttac cgccagtctg
     3961 gccgagtctc cccgtgtgtc gaggatcatc ttctgatcct tattgaaccc aaggtcctgg
     4021 ctgcgcatat aatggagctg gttgtatacg acgagggtgg cgatgatcag cccggttgcg
     4081 atggtaaact ggacgatgac gagtgccttg cgtaaaaaga tgccccttgt cccggtggca
     4141 aaacgtcctt tgagcacctc caccggctga aaggaggaga gcaccagcgc cgggtagatg
     4201 cccgcggcga tcccgatgcc gatggcgatc cccagcagga tcagcaggtc actgccctgc
     4261 gagaagatgc cggggctgac gatcttcccg gcaatctgat tgaagggatg gatcagcagc
     4321 gcggtcagga tcaccgccat gaggaaggcg atcaggcaaa ggatgatgct ttccccgatg
     4381 aactgccggg ccaattgccc tttcaccgca cccgccactt tccggatacc cacctctttt
     4441 gcgcggtcgg tcgaccttgc cgtcgtcagg ttgatgaagt tgatacaggc gatcagcagg
     4501 atgaacacgc cgatgatgga aaagatatag acatcggtga tattgcctgt tttggagcca
     4561 tctctcgtgc tgcgcaggta gacatccctc aaccgttcga gatagagtgt aggcaccatc
     4621 ttgctttccc gcatttccct gccgtccatc ttctcgagga aggccgggaa ttttttctcc
     4681 agtgcggcgg cgctggcccc gggcctgagc agcacatacc cgttgggtcc atagtttccc
     4741 cattgatcat cgatacccgg gttgagccgc cgggtaatgg tgttcatgga aacgaccatg
     4801 ctggcgcgaa tggtggagtt gtccgggata tccttcatga cgccggtgac ggtcgcatgg
     4861 aatttctggc gggtcaggat cagggactgg cccatcgggt ccgcattgcc aaaaaaggtc
     4921 ttggcggtct tttcggtcag cacgacgcta aacggttctt tcagcgccgt tttacggtct
     4981 cccctgagca ggggaagatc gaacacctgg aaaaaggtcg aatccgccgt cgcgacatcg
     5041 tcctgcgcga attgctgatc tccccggtag acgagccagt tatctcccat cccgagccgc
     5101 agcgctgctt ccacctccgg cagttcggcc gtcaggtgcg ggggcaccgc ccaggaaggc
     5161 tggctggcat gaatagtctc ggtgggtgtc ttgatatcgc aaacgatccg gtagacgcgg
     5221 tcggcctttg tatacatcga atcatagctc agctcgaagc ggacatagag gaaaatgagg
     5281 aagaaagcga ccatgccgac agtcagtccg agaatattga ggagggaaaa acctttatgc
     5341 cgccagagat tgcggaaggc gattcgcagg tagtttcgaa gcatatgggg gagtttggct
     5401 taaagtacca aaaaaccccc ggatgttgca aagctacctt aggcggtcgg cacttttaac
     5461 taatttttcg tccttcctga tcccgccggc ggcgaaaagg aggcatacgg ggatggcggc
     5521 cgacagcagg gcgccgatat tatagctgcc ttc
//
LOCUS       DATGFG010000921         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_179857, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000921 DATGFG010000000
VERSION     DATGFG010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179857"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2799
                     /locus_tag="VKQ52_17450"
     CDS             <1..2799
                     /locus_tag="VKQ52_17450"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HLZ89047.1"
                     /translation="ASGLSILFLFWTITHFARKLTSHTFTILATGVVGALAYTFCDSF
                     WYSAVEGEVYATSAFFTAIVFWAILKWEQQADNRGADKWLIFIFYMMGLSIGVHLLNL
                     LTIPAIVMVYYFKRYRPTILGSIVALLVGCAITGFVQVVVIQYTIRGAGAFDIFFVNS
                     LNLPFFTGFATFFILLAAAFIVGIRWAVRRNKYYLKIGLWGAAFMLLGYSTYFTTMIR
                     SNADTPVDIYNVDNPVSLVGYLSREQYGDWPILYGPDFTDQPPTVEAGDFYVKGADRY
                     EAAGKKYAQDWAHAPSAHFFPRMYNSDNTRGDVDTYKRFSGMADGDNPTMADNIKYFL
                     RYQTNWMYLRYFLWNFAGKQNDLQGFGNPRDSNWVSGISFVDNLLYGDQSQLPDTAHK
                     NNKSYNRLYMLPFLLGLLGLFVQYRHRRRDALVTGLLFFFTGWAIVIYLNQAGLQPRE
                     RDYAFAGSFYVFAIWIGLGVIGVQQGIKRLIPRIPRSAGAWSAAATCLLAVPILMASQ
                     EWDDHDRSKKTLARDLAADYLESCPPNAILFSSADNDTYPIWYAQEVEGIRPDVRVII
                     NTLSGTDWLINSLRYKINKADAVDVLFTPEEIMGDKKAAVYFTDGVAGFNKDSYYDLY
                     DTFKTVLAKDDPRYMTESTSGTPLNILPTRKFSIPVDARQVLANGTVHADEKVVPQVQ
                     FDISPQKRLLMKNELTMLAIIAANRWHRPICFTSTQDINALGLTKYARLRGLTYELTP
                     IEDSRVDNDVAYKNVMTKFQYGHAGTPGVYFDEANRRELNLLKLSHAEIAGSLAAAGR
                     KEDARRVLENYDHNVDIANYSYGMTSYLGNFDNSYSLYFLQSCYAADDIALARKVAAS
                     MKKDLHQQMQYYQSLGESMTDEQLAISAQQAIQGKANNLSDKQQDFAQDILSSYQILL
                     QMAQLENQYKASVSKPPM"
     gene            complement(2810..>3654)
                     /locus_tag="VKQ52_17455"
     CDS             complement(2810..>3654)
                     /locus_tag="VKQ52_17455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLZ89048.1"
                     /translation="SDAACALPPQVGSNTLSLPAVPVPVQGVGIDASATAICQDSLVQ
                     FVAVPDNGGSDPAYQWQVNGVNTGSGPTFSDAGLTNGDVVNCVMTGSLTCSQPVNANA
                     MVTMTVYPLPVITLDSVVIIAGGSATRLMPAVTGDVVRWNWSPAVGLDDASAAEPFAS
                     PVITTSYQLYVVTAEGCHSTASELVEVYYPLRMPGAFTPNGDGRNDVFRVPPSSPVTI
                     RYLTVYNRQGARLFYTANVSTGWDGSYNGVSQPAGVYVWELVFDNPLTKKEESRKGTV
                     VLVR"
BASE COUNT          844 a         1291 c          811 g          708 t
ORIGIN      
        1 gccagcggcc tttccatcct cttcctgttc tggaccatca cccattttgc gcgcaagctc
       61 acctcccata ccttcacgat cctcgccacc ggcgtggtgg gcgcactcgc ctacaccttc
      121 tgcgactcct tctggtatag cgccgtcgaa ggggaggtct acgctacctc cgcctttttc
      181 accgccatcg tcttctgggc catcctcaaa tgggaacaac aggccgataa tcgcggcgcc
      241 gacaaatggc tcatcttcat cttctacatg atgggcctct ccatcggcgt ccacctcctc
      301 aacctcctga ccatccccgc tatcgtcatg gtctattact tcaaacgcta ccgccctacc
      361 atcctgggct ccatcgtcgc cctcctcgtc ggctgcgcca tcaccggctt cgtccaggta
      421 gtcgtcatcc aatacaccat ccggggcgcc ggcgccttcg acatcttctt cgtcaactcc
      481 ctcaacctgc ctttcttcac cggcttcgct accttcttca tcctgctggc cgccgccttc
      541 atcgtcggca tccgctgggc cgtaagacgc aataagtact atctcaaaat tggtctctgg
      601 ggcgccgcct tcatgttgct cggatattcc acctatttca caacgatgat ccgcagcaac
      661 gccgacaccc cggtcgacat ctacaacgtg gacaaccccg ttagcctcgt cggctatctc
      721 agccgggaac aatacggcga ctggcccatc ctctacggcc ccgacttcac cgaccagccc
      781 cctaccgtgg aagccggcga cttctatgta aaaggcgccg accgctacga ggccgccgga
      841 aaaaaatacg cccaggactg ggcccacgcc ccttccgccc atttcttccc gcgcatgtac
      901 aacagcgata acacccgcgg cgacgtcgat acctacaaac gcttctccgg catggccgac
      961 ggagacaacc ccaccatggc ggacaatata aaatacttcc tccgctatca aaccaactgg
     1021 atgtacctcc gctatttcct atggaatttc gccggtaaac aaaacgacct ccagggcttt
     1081 ggcaacccac gggacagcaa ctgggtcagc ggcatctcct tcgtggacaa cctgctctat
     1141 ggcgaccaaa gccaactgcc cgacaccgcc cataagaaca acaagtcgta taatcgcctc
     1201 tatatgttgc cctttctgct ggggctgctg ggactcttcg tacagtatcg ccaccgccgc
     1261 cgcgatgccc tggtaaccgg gctgctcttc ttcttcacgg gatgggccat cgtcatctat
     1321 ctcaaccagg ccggcctgca accccgcgaa cgggattatg ccttcgccgg cagcttttat
     1381 gtcttcgcca tctggatcgg cctcggagtc ataggcgtac aacaaggtat taaacgcctc
     1441 atccctcgca ttccccgatc cgccggagca tggtcagccg ccgccacctg cctgctcgcc
     1501 gtcccgatcc tcatggcctc ccaggaatgg gacgatcatg accgtagcaa aaagaccctg
     1561 gcccgcgacc tcgccgccga ctacctcgaa tcctgcccgc ccaatgcgat cctcttctcc
     1621 tccgcagaca acgacaccta ccccatctgg tacgcccagg aagtggaagg catccgcccc
     1681 gatgtccgcg tcatcataaa taccctctcc ggcaccgact ggctcatcaa ctctttacgc
     1741 tataagatca acaaggcgga tgccgtcgac gtcctgttca cgccggaaga gatcatgggc
     1801 gataaaaaag cggcggtata cttcaccgat ggcgtggcag gcttcaacaa ggacagctac
     1861 tatgacctct acgatacctt caaaaccgta ctggcaaagg acgatccacg ctatatgacg
     1921 gagtccacca gcggcacgcc cctcaatatc ctcccgaccc ggaaattctc catacccgtg
     1981 gacgcccggc aggtgttggc caacggcacc gtacatgcgg atgaaaaagt cgtcccccag
     2041 gtccagttcg acatctcccc ccagaaacgc ctcctgatga agaacgaact caccatgctg
     2101 gcgatcatcg ccgccaaccg ctggcatcgc cccatctgct tcacctctac ccaggatatc
     2161 aatgcgctcg ggctgacaaa atatgcccgc ctccgcggat tgacctacga gctgacgccg
     2221 atagaggata gtcgcgtgga caacgatgtt gcctacaaga acgtcatgac caaattccaa
     2281 tacggccacg ccggcactcc cggggtctat ttcgacgaag cgaaccgccg ggaactcaac
     2341 ctcctaaagc tgtcccacgc cgaaatagcc ggcagcctcg ccgccgccgg tcgtaaggaa
     2401 gatgcccgtc gcgtactcga gaactatgac cacaacgtcg acatcgccaa ctactcctat
     2461 ggcatgacca gctacctcgg gaattttgac aacagctact cgctctactt cctccaatcc
     2521 tgctatgccg ccgatgatat cgccctggcg cgaaaagtcg ccgcctcgat gaaaaaagac
     2581 ctccaccagc aaatgcaata ctaccagtcc ctcggcgaaa gcatgaccga cgaacaactg
     2641 gccatcagcg cccagcaggc aatccagggg aaagccaaca atttatccga taaacaacag
     2701 gatttcgccc aggacatctt gtcaagctac caaatactct tgcaaatggc ccaactcgaa
     2761 aatcagtata aagcatcggt aagtaaacca ccgatgtagg cgctggatct tacctcacta
     2821 aaaccaccgt tcccttcctg ctctcttcct tcttcgtcaa cgggttgtca aacaccaact
     2881 cccacacata cacgcccgcg ggctgcgaaa ccccgttgta acttccatcc catcccgtcg
     2941 atacattggc ggtataaaac aaccgggccc cctgacggtt ataaacagtc aggtaacgga
     3001 tcgtcaccgg cgacgacggg ggcacccgga aaacatcatt tcgtccatcc ccgttcggcg
     3061 taaaagcccc cggcattcgc agcggataat acacttccac caactccgat gccgtcgagt
     3121 gacaaccctc cgccgtcact acataaagct ggtaactcgt cgtgatcacc ggcgacgcaa
     3181 acggctcggc agccgaagca tcatccaacc ccacagccgg actccaattc cagcggacga
     3241 catcgcccgt aacggcaggc atcaaccggg tcgccgatcc gccggcaata ataaccaccg
     3301 aatccagggt gatcaccggc aacggataaa cagtcatcgt caccattgcg ttcgcattca
     3361 caggctgact gcaggtaaga ctaccggtca tgacacagtt gaccacatcc ccgttggtca
     3421 aacccgcatc actaaaagtc ggcccactac cggtatttac cccgttcacc tgccactgat
     3481 acgccggatc acttccccca ttatccggca cggccacaaa ttgcacaaga ctatcctggc
     3541 agatagccgt cgccgaagca tcgatgccga cgccttgcac cggcaccggc acagccggca
     3601 gactcagcgt gttcgaaccc acctggggcg gtaacgcaca cgcggcgtca ctcg
//
LOCUS       DATGFG010000922         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1419705, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000922 DATGFG010000000
VERSION     DATGFG010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419705"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1480)
                     /locus_tag="VKQ52_17460"
     CDS             complement(32..1480)
                     /locus_tag="VKQ52_17460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ89049.1"
                     /translation="MLPSSLPTRILIVEDDEDDFLIIEACIKDIPDKGFHIDWCYDYN
                     EALHRISQGAYDLYFVDYLLGAKTGLELLRDAIALGCEEPLVLLTGIGNRDLDVQAMT
                     IGAVDYLVKSEINTEKLERSIRYALERSAYIKALRNNERKFRNIFERSKDAVFLTGDD
                     LVFRDVNAATCDLFKYGKDELLQMSLYNLFARTEAAEQLRNRLSLTGEVEDLEVELIT
                     RNKERRNCILSISWQVYSSGETYIQGIIHDITNLKRIERATFQIEKLRSTATLLRTLA
                     HEVRNPLTNINLSVEQLKPEMNSEEANIYLDIITRNCGRIDGLISELLDLSRPAEISL
                     QKTRLQEVLDNTLAAASDRISLKNIRLELVYPEKPAFVMADKEKLRIAFLNILINAVE
                     AVPAQSGVITIAIREEIPQHYRVSINDNGGGIPEENISRIFEPYFTSKTNGFGLGLAA
                     TWNILQSHRAGIDVNSQLGEGTSFMLTFEQAT"
     gene            complement(1493..2779)
                     /locus_tag="VKQ52_17465"
     CDS             complement(1493..2779)
                     /locus_tag="VKQ52_17465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ89050.1"
                     /translation="MQPKIMLVDDREDNLLSMETVLESDGYRFVKATSGRQVLKYLLT
                     DFDFALILMDVQMPNLNGFETASLIYERERLRHIPIIFITANNYGEENLFKGYRAGAV
                     DYIYKPVNPELLRVKVSIFVDLYRKNQRLIAQEQKLVAINRSLEMEVSERKASEEKVT
                     ELNKQLLENISRLETANKDLDLFAFMASHDLQAPLRKIRMFSDRLLASTDNNFGKEGK
                     LYLTRIQEVSKRMQELINDILRFSKISVEKQNFEEVDLNGVVGEVLSELEGVIREKDA
                     LVTVDRLPVLPASTVLMGPLFSNLISNSLKYSKKKEPPQIRIRYEEGPAIASAGGREP
                     ETRYGRIYIEDNGIGFDQKYAEQIFDMFRRLHSNAEYEGTGIGLALCKKIVEMHKGFI
                     SALGRPGEGAVFIVSLPLSGVQHELVEKNDDISDLN"
     gene            complement(2801..>3914)
                     /locus_tag="VKQ52_17470"
     CDS             complement(2801..>3914)
                     /locus_tag="VKQ52_17470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ89051.1"
                     /translation="AHQYDMKAVVATNYLEVFDFINRFTPIAITLDVKLPDTSGWKVL
                     DLLRNDLNYRHIPIHLISGEENRTLALKRGARSFLLKPLENEALNELFEDIRSFSKKE
                     TRRVLVVEDNELDSSQISKMLQSENMDVTIASNGRKALEETDIKTFDCIILDYTLPDI
                     SGVDLVTRVSQSKNKLTPVIVYSAKDFNKKELYHLNRSSNTVLLKGVNSLEHLLEETV
                     LHLHINHRELAPDKRRVIEQLRMKDDILTGKNVLVVDDDVRNLFALTTVFERYNINVI
                     TAESGKEAIQIINDDKQKVDMVLMDIMMPEMDGYETTQKIRREHKNNTLPIIAVTAKA
                     MKGDRQKCIEAGASDYITKPLKIDQLLSLMRVWFYK"
BASE COUNT          827 a         1202 c          940 g          945 t
ORIGIN      
        1 agatactcga tctggaaccg atcgcctacc cctaggtggc ctgttcgaag gtgagcataa
       61 aactcgtccc ctctcccaac tgggaattta cgtcgatccc tgcccgatgc gattgcagga
      121 tattccaggt cgcggccagc ccaagcccga acccattggt cttggaagta aaatacggtt
      181 cgaagatccg ggagatattc tcctccggga taccgccccc attgtcattg atcgataccc
      241 tgtaatgctg gggtatctcc tcgcggatgg cgatcgtgat cacccccgac tgcgccggga
      301 cggcctccac cgcgttgatg aggatattga gaaaggcaat tctgagcttc tccttatcgg
      361 ccatcacaaa ggccggcttc tccgggtaca ccagctccag ccggatattc tttagcgaaa
      421 tacggtccga cgccgcggcc agggtattgt caagcacttc ctgcaaccgg gtcttctgaa
      481 gcgagatctc ggcaggccgc gaaaggtcca gcagctcgga gatcagcccg tcgatccgtc
      541 cgcagtttcg cgtgatgata tcgagataga tattcgcctc ctcgctgttc atctccggct
      601 tcagctgctc caccgagagg ttgatattcg taagcgggtt ccgcacttca tgcgccagcg
      661 tacgcaacag cgtcgccgta gaccgcagtt tctcgatctg aaaggtcgcc cgctcgatcc
      721 gcttgagatt ggtgatgtca tggatgatcc cctggatata ggtctcgccg ctggaataca
      781 cctgccagga gatcgacagg atacagttcc gccgctcctt gttccgcgtg atcagctcca
      841 cttcgagatc ctccacctcc ccggtaagcg acagccggtt ccgcaactgc tccgccgcct
      901 cggtccgcgc aaaaagattg tacaggctca tctgcagcaa ttcatccttg ccgtatttga
      961 aaagatcaca ggtggcggca ttcacatccc ggaaaacaag atcgtctccc gtcaggaaaa
     1021 cggcatcctt gctccgctcg aagatgttcc ggaattttcg ttcattgttg cgcaatgctt
     1081 ttatatacgc actcctttcc agtgcgtacc ggatactgcg ttccagcttc tcggtattga
     1141 tctcggattt taccaggtaa tccaccgccc cgatggtcat ggcctggaca tccaggtcac
     1201 ggttgccgat gcccgtcaac agcacaagcg gctcctcaca ccccagcgca atggcatcgc
     1261 gtaataattc cagcccggtc ttcgccccca acaggtagtc cacaaaataa agatcatagg
     1321 cgccctggct gatcctgtgc agcgcctcat tataatcata gcaccagtcg atatggaatc
     1381 ccttgtcggg aatatccttg atacaggcct ctatgatcag aaagtcgtct tcgtcatctt
     1441 ctacgatcag tatccttgta ggcaggctgg aaggcaacat gatgcgaatg ggttaattta
     1501 gatcactaat gtcgtcattt ttttcaacta attcatgctg cacaccactg agcggaaggg
     1561 atacgataaa tacggcgccc tcccccggcc tgccaagcgc agaaataaaa cccttgtgca
     1621 tctccacgat cttcttgcaa agcgcaagcc cgattcccgt accctcatat tccgcattgg
     1681 aatgcagccg ccggaacata tcgaagatct gttccgcata tttctgatca aagccgatgc
     1741 cattgtcctc gatatagatc cgcccgtagc gcgtctccgg ctcgcggccg ccggcagacg
     1801 cgatcgccgg cccttcctcg taccggatac ggatctgcgg cggctccttt tttttgctgt
     1861 actttaagga gttgctgata agattggaga aaagcggccc catcagcacc gtactcgcgg
     1921 gcaacaccgg cagccggtcc accgtaacca acgcatcctt ttcccggatc actccctcca
     1981 gctccgacaa cacctccccg acgaccccgt tgagatccac ttcctcgaag ttctgctttt
     2041 ccaccgaaat cttggagaac cgcaggatat cattgatcag ctcctgcatc cgcttcgaca
     2101 cctcctggat ccgcgtcagg taaagtttcc cctccttccc aaaattgtta tcagtactgg
     2161 ccagcagccg gtcgctgaac atccggatct ttcgcagcgg cgcctgcagg tcatgcgaag
     2221 ccatgaacgc aaaaagatcc aggtccttgt tcgccgtctc cagcctcgag atattctcca
     2281 gcagctgttt gttcagctcc gtcacctttt cctccgaagc cttccgctcg ctgacctcca
     2341 tttccaggct gcggtttatc gccaccagct tttgttcctg ggcgatcagc cgctggttct
     2401 tgcggtacag atccacgaag atcgacacct tcacgcgcag caactcggga ttcacgggct
     2461 tgtagatgta atccacggct cccgcgcgat aacccttgaa caggttctcc tcgccataat
     2521 tgttggccgt gatgaaaatg atcgggatat gccgcagccg ctcccgctcg tagataaggg
     2581 aggccgtctc gaaaccattc aggttgggca tctggacatc cataaggatc agcgcgaagt
     2641 cgaaatccgt caggagatat ttcagcacct gccggcccga ggtcgccttt acaaagcgat
     2701 acccgtcgga ttccagcacc gtctccatcg acaggaggtt atcctcccgg tcatcgacta
     2761 acatgatctt tggttgcatc atcgaaattt aggatgggga ttatttatag aaccagacgc
     2821 gcatcagcga caataactga tctatcttca gcggcttggt gatataatcc gaagccccgg
     2881 cctcgataca tttctgccgg tcacccttca tggccttggc cgtcaccgcg atgatcggca
     2941 gggtattgtt cttgtgctcc cgccgtatct tctgcgtggt ctcatacccg tccatttccg
     3001 gcatcatgat atccataagc accatgtcca ccttttgctt gtcgtcattg atgatctgga
     3061 tcgcctcctt gccgctctcc gccgtgatca cattgatatt atagcgttcg aacaccgtcg
     3121 tcagcgcaaa caggttacgg acatcgtcat ccaccaccag tacgttcttc ccggtaagga
     3181 tatcgtcctt catccggagc tgctcgatca cccgccgctt gtccggcgcc agctcccggt
     3241 gattgatatg cagatgcagc acggtctcct ccagcagatg ctccaggctg ttgaccccct
     3301 tcagcaacac cgtattcgaa ctgcggttca gatggtacag ctcttttttg ttgaaatcct
     3361 tggcggaata gacgatcacg ggcgtcagct tgtttttcga ctggcttacc cgcgtgacga
     3421 gatcgactcc ggagatatcc ggcagggtat aatcgagaat gatacaatca aaggtcttga
     3481 tatcggtctc ctccagcgcc ttccggccgt tggacgcgat cgtcacatcc atgttctcac
     3541 tctgcagcat cttgctgatc tgtgaggaat ccagttcatt gtcctccacc accagcaccc
     3601 gccgggtctc cttcttgctg aaggaccgga tgtcttcgaa cagctcgttc aacgcctcgt
     3661 tctccagcgg cttcagcagg aaactgcggg cgccccgctt caacgccagc gtccggttct
     3721 cttccccgga gataagatgt atcgggatat gccggtaatt gagatcgttc cgcaagaggt
     3781 ccagcacttt ccagccactg gtgtcaggca gtttgacatc gagcgtgatc gcgatcggcg
     3841 tgaaccggtt gataaaatcg aacacctcca ggtagttggt cgccaccacc gccttcatgt
     3901 cgtattgatg cgcc
//
LOCUS       DATGFG010000923         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_991892, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000923 DATGFG010000000
VERSION     DATGFG010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991892"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VKQ52_17475"
     CDS             <1..763
                     /locus_tag="VKQ52_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89052.1"
                     /translation="VGTTTPSQALFAYVEGQDTARKGTAPAGTGAKPATPNPPRRGNK
                     EGDRRLQVSINAQSNVFDVLDTFRITFSSGLKVFDSTQVRFTDDGYHDIDARQYHYVR
                     DTSNKVFTLYYVWPTDVKYHLILPRTFGQDSLGRKLLKDDTLSFRTKKDIDYGEIRIR
                     VLNLDMSQHPVLQFVQSDKIKYTLPFNNRREVRRLLFSPGEYELRVLYDVNRNLIWDH
                     GVFFGKNKKQPERVTTIRKKLNVRANWDNDVDITL"
     gene            778..1398
                     /gene="recR"
                     /locus_tag="VKQ52_17480"
     CDS             778..1398
                     /gene="recR"
                     /locus_tag="VKQ52_17480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HLZ89053.1"
                     /translation="MQFSSSLLEQAVNEFAKLPGIGKKTALRLVLHLLRSESSQVQGF
                     GETVIRMRNEIRFCQRCHNVADADICSICANSMRKQELICVVESLREVIAIESTQQYN
                     GVYHVLGGIISPLDGIGPEQLNIETLVSRVRKEETQELIFALNPTIQGDTTIYYIQKK
                     LQDAHVRITTIARGIAFGGELEYADEMTLARSITNRLPVESYRSGG"
     gene            1643..>2307
                     /locus_tag="VKQ52_17485"
     CDS             1643..>2307
                     /locus_tag="VKQ52_17485"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HLZ89054.1"
                     /translation="MEKARKTLEEALKKRILVIDGAMGTMIQRHKLEEKDYRGERFKD
                     WPSDLKGNNDLLCLTQPAIIEGIHRQYLEAGADIIETNTFNSQAISMADYHMEALAYE
                     INVAAARIAKKAAADYTAKTPDKPRFVAGAIGPMSKTLSLSPDVNNPGFRAVTFDEVT
                     SAYYEQVKGLVEGGVDLLLIETIFDTLNAKAAIFAIQKYFRDTHQPELPVSISGTITD
                     ASG"
BASE COUNT          546 a          603 c          663 g          495 t
ORIGIN      
        1 cgtcggcacg accacaccgt cacaggcgct ttttgcctat gtggaggggc aggatacggc
       61 caggaagggc actgcgccgg ccggcacggg cgccaagccc gctacgccga acccgcccag
      121 gaggggcaac aaggagggcg accggcgtct gcaggtgtcc atcaatgccc agagcaatgt
      181 cttcgatgtg ctcgatacct tccggatcac cttctcctcg gggttaaagg tgttcgactc
      241 cacccaggtg cggttcacgg acgacggtta tcatgatatc gacgcccggc aatatcatta
      301 tgtccgggat acgagcaata aggtctttac cctgtattat gtctggccga cggacgtcaa
      361 atatcatctt atcctgccca ggacctttgg ccaggattct ctcggcagga agctgttgaa
      421 ggacgatacc ctgagtttcc ggacgaaaaa ggatatcgac tatggggaga tccgcatccg
      481 ggtgctcaat ctcgacatgt cccagcatcc tgtcctgcaa tttgttcagt cggacaagat
      541 caaatacacc ctgcccttta acaaccggcg ggaggtgcgg cggctgcttt tctcgcccgg
      601 ggagtacgag ctgcgtgtcc tttacgacgt gaaccggaac ctgatctggg atcatggggt
      661 ctttttcggc aagaacaaga agcagcctga aagagtgacg acgatccgga aaaaattgaa
      721 tgtcagggcc aactgggaca atgatgtgga tataaccctg tgacgtacct ttgtcaaatg
      781 caattttctt cgtctttact ggaacaggca gtcaatgaat tcgccaagct gccgggcatt
      841 ggaaaaaaga cggccttgcg gctggtgctg catttgctga ggtcggagag cagccaggtg
      901 caggggtttg gcgagacggt gatccggatg cgcaacgaga tcaggttctg ccagcgctgt
      961 cacaatgtag ccgatgccga tatctgttcc atctgcgcca attcgatgcg gaaacaggag
     1021 ttgatctgtg tcgtggagag tctgcgggaa gtgatcgcga tcgaaagcac ccagcaatat
     1081 aacggggttt accatgttct tggcgggatc atttccccgc tggatgggat cggcccggag
     1141 cagttgaata tcgagacgct ggtctcgcgg gtcaggaaag aggagaccca ggaactgatc
     1201 tttgccttga accccacgat ccagggggat acgaccattt attatatcca gaagaaacta
     1261 caggacgccc atgtccggat cacgacgatc gccaggggta tcgcctttgg cggggagctg
     1321 gaatatgccg atgagatgac gctggcgcgg agtatcacca accgccttcc ggtggaaagc
     1381 taccggtcgg ggggatagcc gtgccaggag tcgggcgtcg tggtcatggg gggcgggtcg
     1441 ggcggctgac attgaccgta tcattatttt gcataacttt gcatcctcag gcggatttgt
     1501 ttattgttca gcctgttgaa tctaattacg gaccggactt tgtccatccg aacaaaaatt
     1561 gaactaataa acggtcgaaa tatcccacct atctttggtt atttccttcg ttatcaatta
     1621 gttctacttg gaggaataac acatggaaaa agcacgcaaa acactggaag aagccctcaa
     1681 aaaacgcatc cttgtcatcg acggggccat gggtacgatg atccagcgcc acaagctgga
     1741 agagaaggat tatcgtggtg agcgctttaa agactggcct tccgatctga aagggaacaa
     1801 cgatctgttg tgtctgaccc aaccggccat catcgaaggt atccaccggc agtacctgga
     1861 ggcgggcgcc gacatcatcg agacgaatac cttcaactcc caggcgatca gtatggctga
     1921 ttaccacatg gaggcgctgg cctatgagat caatgtggcc gctgcccgta tagccaaaaa
     1981 ggcggcagcc gattataccg cgaagacccc ggacaaaccc cggttcgtgg cgggcgccat
     2041 cggtcccatg agcaagacgc tctcgctctc gccggatgtc aataaccccg ggttccgggc
     2101 ggtaaccttc gatgaggtga cgagtgcgta ttacgaacag gtaaagggcc ttgtggaagg
     2161 cggcgtcgat ctcctgctga tcgagaccat ctttgacacc ctgaatgcca aggccgccat
     2221 tttcgccatc cagaaatatt tccgcgatac ccatcagccg gagctgccgg tgtcgatcag
     2281 cggcaccatt acggatgcca gcggaag
//
LOCUS       DATGFG010000924         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_853238, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000924 DATGFG010000000
VERSION     DATGFG010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853238"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VKQ52_17490"
     CDS             <1..293
                     /locus_tag="VKQ52_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89055.1"
                     /translation="RAAAFTEASALRRTGFIAQEVEQAAGEAGYDFTGVSRPKNEHDH
                     YSLDYQAFIMPMVKAMQEQQKMIETLKGQNDEQQKRLDAMQAELDAIKAKMR"
     gene            428..2260
                     /locus_tag="VKQ52_17495"
     CDS             428..2260
                     /locus_tag="VKQ52_17495"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HLZ89056.1"
                     /translation="MKRRIHLMLALSVFALARTEAQNVGVGTTEPKQKLHVAGGLRVD
                     TLSNGRDSGLVRHDATGAVTSLRFTGDSTQVLRGNGTFGAAAVIVTSALTDPWLVGGN
                     AGIVPAINFLGTTDNAALRLRINNQWAGQLDSVSQSTFFGYGAGKNDTAAGAGNTAFG
                     YQTLTVVTSGAFNTATGVRALLNTSTGVYNTATGWQALLQNTSGAQNTAMGVQALQSN
                     TTGGNNTAVGLNSLNVNSSGSNNTALGVQALNFNTQGNYNIGVGRSAGYGNTTGNNNI
                     AIGRAALLTNQTGYNNLAIGTGALQKNLGSADEVAIGDSALFTETGGNTQNTALGTKA
                     MFSNTSGSYNTALGAWGLYANTTGSYNCSVGGALANSTTGNSNVGVGITALVSNTSGS
                     DNAGVGYNAIGINSTGNNNSALGFYALGSNISGANNTAVGSGADVSSGNLSNATALGA
                     GAVVNASNKVRIGNSSVTVIEGQVLPTTPSDGRFKFNIKEDIKGLDFILRLRPVSYQF
                     DVRSFDGPNGAAYTRYAAYDEAMALRRTGFIAQEVEKAAQAAGFDFTGVERPKNERDH
                     YSLSYPAFIMPMVKAIQEQQQQIDDLKKTVAEQRRMIDELLHHQ"
BASE COUNT          559 a          612 c          760 g          449 t
ORIGIN      
        1 tgcgggcggc ggcttttacc gaagcttcgg cgttgcgtcg caccgggttt attgcccagg
       61 aggtagaaca ggccgccggg gaagccgggt atgattttac cggggtctcc cggcccaaaa
      121 atgaacacga ccactatagt ctggactacc aggcttttat catgccgatg gtaaaggcga
      181 tgcaggagca gcagaagatg atcgagacgc tgaaaggcca gaacgacgaa cagcagaaaa
      241 ggctggatgc gatgcaggcg gagctcgatg cgataaaggc gaagatgcgg tgaaagtgaa
      301 gttgcggtga agacgaggat gcggtgaagg cgctttgcgg tgaaggcgaa gatgcgacga
      361 aagtgaggat gcggtgaagg agccggcggg acgttgaaca ctgataaacc aaaaaacccc
      421 ttatacaatg aaaagaagaa ttcatttgat gcttgcctta tcggtatttg ccctggcaag
      481 aacagaagcg cagaatgtcg gcgtcggaac gaccgagccc aaacaaaaat tacatgtggc
      541 cggcgggctg cgggtcgaca cgttgtcgaa cgggagggat agcgggctgg tgcggcatga
      601 tgccaccgga gccgtgacca gccttcgatt caccggtgac agcacccagg tgttgcgcgg
      661 caatggcacc tttggtgccg cggcggtgat cgtgacttcg gcgctgaccg atccgtggct
      721 ggtgggcggc aatgcgggga tcgtcccggc gatcaacttc ctggggacga cggataatgc
      781 ggccctgcgt ctacggatca ataaccagtg ggcgggccag ttggattcgg tgagccagtc
      841 cactttcttc ggctatggag cgggtaagaa cgatacggct gccggcgccg gtaatacggc
      901 gtttggctac cagacgttga ctgtggtgac cagcggcgct ttcaacacgg ctacgggggt
      961 tcgggcgttg ctgaatacct ccacgggtgt ctacaacacg gccacggggt ggcaggcgct
     1021 gctacagaat acctcggggg cgcagaatac ggcaatgggt gtacaggccc tgcagagcaa
     1081 tacgacaggc ggcaataata cggccgtagg actgaattcc ttaaatgtca attcctcggg
     1141 ttcgaataat accgcgttgg gggtacaggc gctgaatttt aatacgcagg gtaattataa
     1201 tatcggcgtg ggccggtcgg cgggctatgg gaatactacc ggcaataata atatagccat
     1261 tggacgtgcg gcgttgttga ccaaccagac cgggtataac aacctggcca tcggcacggg
     1321 ggccttgcaa aaaaatctgg gtagcgccga tgaggtcgcc atcggcgatt cggctttgtt
     1381 cacggaaacc ggcggcaata cccagaacac tgccctgggc accaaggcga tgttctccaa
     1441 taccagcgga tcatacaata ccgccctggg cgcatggggg ctctatgcca atacgacagg
     1501 ctcttacaac tgttcggtcg ggggtgccct tgccaatagc acgacgggca attccaatgt
     1561 gggcgtaggc attacggcgc tggtgagcaa cacctccggc agcgacaatg ccggcgtcgg
     1621 gtacaatgcc attggcatca acagcaccgg gaacaataat tccgcattgg gattctatgc
     1681 gctggggtcc aatatttccg gcgccaacaa tacagccgtc gggtcgggcg cggatgtctc
     1741 cagcggcaat ctcagcaacg ccacggccct cggcgcgggc gcggtggtga atgcttccaa
     1801 caaggtgcgt atcggcaaca gcagcgtcac cgtcatcgaa gggcaggtgc tgccgacgac
     1861 gccatcggat ggccgattca agttcaatat aaaagaggat atcaagggcc tggactttat
     1921 tctgcgactg cggccggtga gctaccagtt cgacgtcagg agcttcgatg gaccaaatgg
     1981 cgccgcctat acccgatacg cagcttacga cgaggccatg gcgctccgcc gcacgggctt
     2041 tatcgcccag gaagtagaga aggcagcaca ggcggcaggg tttgatttta ccggtgtcga
     2101 gcggccgaag aacgaacgcg atcactacag cctgagctat ccggccttta tcatgccgat
     2161 ggtgaaagcc atccaggagc agcagcagca gatcgacgat cttaagaaga ccgtagcgga
     2221 gcagcggcgg atgatcgatg aattgttaca tcaccaataa aaacctgatt atgctcagaa
     2281 atttgttttt cgcgttggcc ctgctggcgg gtaccgcggg ccacgcgcaa ttgaacgtac
     2341 aggacagggg catcgtcttc ctggcgccgg gggccgtgct
//
LOCUS       DATGFG010000925         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1143864, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000925 DATGFG010000000
VERSION     DATGFG010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143864"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1421
                     /locus_tag="VKQ52_17500"
     CDS             264..1421
                     /locus_tag="VKQ52_17500"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HLZ89057.1"
                     /translation="MPHYYSLGSIPHKRHTQFHQPSGSLYSEQLFSTEGFSNDYSLLY
                     HVHPPTEIIGTDEPVDVQPRIAEEKMLKHRSFEGFSIKPEADYLRSRRPVLVNSDCHI
                     VLAAPQQSMEDYFYKNADADELIFIHQGRGVLRTMYGELSFNYGDYLVVPRGTIYQMR
                     FEDADNRLFIVESFSPIRYPRKYLSPYGQLLEHSPYCERDIRGPQSLATRDEKGDFLI
                     RTKKKGRLYGIHYGHHPFDVIGWDGCCYPFAFSIHDFEPITGRVHQPPPVHQTFQGPS
                     FVVCSFVPRKLDYHPQSIPVPYNHSNIDSEEMIYYVNGNFGSRRGVERGSITLHPRGI
                     AHGPHPGAVEKSLGVDRTEEMAVMVDTFHPLHLTTSSQSIDDPLYPYSWLD"
     gene            1631..>2376
                     /locus_tag="VKQ52_17505"
     CDS             1631..>2376
                     /locus_tag="VKQ52_17505"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89058.1"
                     /translation="MTTEPRIIKFYETTLRDGEQTPGVNYFPDEKLAIAQALADMGFD
                     TLDCGFPISSPGEFEAVRLIASKIENAEICAIGRANPADIERAWEAVKIAAHPVIQPF
                     ISVSPLHRQVKLGKTRDEVRELAGAAVAQARTYTENVDFALEDTTRTEHDFILEICDV
                     LLKEGVRFITLCDTVGFALPWEFGQLVTDVRQEFPGVRISAHCHDDLGLAVANALEAL
                     RNGAERVDTCINGLGERAGNAASEDVIMAVR"
BASE COUNT          542 a          631 c          630 g          573 t
ORIGIN      
        1 tcgaaatagc ggatgtagag cgtttgaacg gaattgtcct tcagggtttg ttgttccagg
       61 ctgtcgggtg agaaagcggt ccgccagtaa tagaagctca tggttttgga ccggtgatgg
      121 caggcggtgg tacaaaagac aaggtagagc agcagcaatc gaaaatgggt gttgggcacc
      181 gggctaatta tttttccata aaggtagggg gaaacggacc ataagaggga ggatttaatt
      241 aattttaacc caacgatctg tatatgccgc actactattc actggggagc attccccata
      301 aacgtcacac ccaattccat cagcccagcg gctcccttta ttcggagcag ttgttctcca
      361 cggaaggttt ttcaaatgat tattcccttc tttaccatgt gcatccgcct acggagatca
      421 tcggtaccga cgagcccgtg gatgtacaac cgcggatcgc ggaagagaag atgttaaagc
      481 atcgcagttt cgaggggttc agcataaagc cggaagcgga ttatctgcga agccgccggc
      541 cggtgctggt gaacagcgat tgtcacatcg tgctggctgc gccgcagcaa agcatggaag
      601 attattttta taagaatgcg gatgccgacg aactgatctt tattcaccag ggccgggggg
      661 tgttgagaac gatgtatgga gagctgtctt tcaattatgg cgattacctg gtggttccga
      721 ggggaaccat ttaccagatg agattcgagg atgcggataa ccggcttttt atcgtcgagt
      781 cctttagtcc tatccggtat ccccggaaat acctgagccc gtatggacaa ttgctggaac
      841 attctcctta ttgcgagcgg gatatccggg ggccgcagtc cctggccacg cgtgatgaaa
      901 agggggattt cctgatccgg acaaagaaga aggggcggct atatgggatc cattacgggc
      961 atcatccctt tgatgtgatc gggtgggacg ggtgttgtta tccatttgcg ttcagcattc
     1021 atgattttga gcctatcacc gggcgcgtcc atcagccgcc tccagtccat cagaccttcc
     1081 agggtcccag tttcgtggtt tgctcttttg tgccccgtaa gctggactac catccacaat
     1141 cgatcccggt tccgtataac catagtaata ttgatagcga ggagatgatc tactacgtca
     1201 acggcaactt cggtagtcgc cgtggtgtcg agcgcggctc cattaccctg catccgcgcg
     1261 gcatcgccca cggaccacat cccggcgccg tcgaaaagag cctgggcgtc gatcgtaccg
     1321 aggaaatggc ggttatggtc gataccttcc atcccttgca cctgaccacc tcctcccaat
     1381 ctatcgacga cccattgtac ccgtatagct ggcttgacta acttcaaata ttttttgaaa
     1441 taaagaaaaa attggaaatt ttttctttat ttcaaaaaat atttcccgtg tcggataaaa
     1501 caacatgaaa cccgtcaaca caatttcgta gggctacccc ctttgagggg gcctgtgtcg
     1561 gatacatagc atgaaacccg taccactttc aatggtttaa aaggaaacat tgccagagga
     1621 gtgaacgatc atgacaaccg aaccacgcat catcaaattc tacgaaacca ctctgcgtga
     1681 cggtgagcaa acgcccgggg tcaactactt tcccgacgaa aaactcgcca tcgcccaggc
     1741 cttagctgat atgggctttg ataccctcga ctgcggcttc cccatctcct cgcccgggga
     1801 attcgaggct gtccgtctca tcgcctccaa aattgagaat gctgaaatct gcgccattgg
     1861 tcgcgccaac cccgctgata tcgagcgcgc ctgggaagcc gtcaagattg ctgcgcatcc
     1921 cgttatccag ccctttatca gcgtttcccc cctccatcgt caggtcaagc ttggcaagac
     1981 ccgcgatgag gtgcgtgaac tggccggcgc cgctgtggcc caggctcgta cctataccga
     2041 gaatgtcgat tttgctctgg aagataccac ccgtaccgag catgacttta tcctggagat
     2101 ctgcgatgtg ttgcttaaag agggcgtccg ctttatcacg ctctgcgata cggtcggttt
     2161 cgccctgccc tgggagtttg gccagcttgt cactgatgtg cgccaggagt tccccggcgt
     2221 gcgcatctcc gcccactgcc atgatgatct cggcctggcc gttgccaacg ctctggaggc
     2281 cctgcgcaac ggtgccgagc gcgttgatac ctgcatcaac ggccttggcg agcgtgctgg
     2341 gaatgccgcc agcgaagatg tgattatggc cgttcg
//
LOCUS       DATGFG010000926         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_618589, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000926 DATGFG010000000
VERSION     DATGFG010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618589"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VKQ52_17510"
     CDS             <1..192
                     /locus_tag="VKQ52_17510"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89059.1"
                     /translation="SGSDDSVRIVPRKQMTLEECMEYIQQDECIEVTPKKIRLRKSIL
                     DEEERKRFQKMLKTEEVGG"
     gene            223..447
                     /locus_tag="VKQ52_17515"
     CDS             223..447
                     /locus_tag="VKQ52_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89060.1"
                     /translation="MRRSNLVFTFIVCLLLTAAALPVKAQCSICTRTAQQLGERPARA
                     LNKGIIYLAVTPLAIFGVIGYRWWKSEQHD"
     gene            440..1375
                     /locus_tag="VKQ52_17520"
     CDS             440..1375
                     /locus_tag="VKQ52_17520"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ89061.1"
                     /translation="MTESRLVSVVLGTYNGEAYLREQLDSVLSQTYSHLEIITIDDGS
                     TDGTVGILREYAARDTRIRVIVNERNLGFIRNFEKGCSLSTGHWISLCDQDDYWMPDK
                     VEKMVNAIGEYPMVYCDSLLCDQDLQPFGNRISDLVHYQSFDDPRQLCVFSRMYGHAT
                     LITRGLFDKARPFREEVPHDGWLAYHATLYGGVWDLPEVLVKYRQHAGNVFGVVGRKK
                     KKPSREEKAGKRKKELARIRVRMQAYCDACPETLVPQKKLLRALVRSYRDFSPGNDIR
                     RVLLFFANYKRLLVVKKYSTGRKWLFCLKMLVKIK"
     gene            complement(1368..>2034)
                     /locus_tag="VKQ52_17525"
     CDS             complement(1368..>2034)
                     /locus_tag="VKQ52_17525"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89062.1"
                     /translation="DPSSSLYYGALLGDRIRMSDWYNHPAVFIRSLATRGSLGGLHPR
                     IIEITDLLKQAPFDHILIETVGVGQNEIEIAGLADTTLVVLAPEGGDDIQSMKAGLLE
                     VADIFVVNKSDRPDADLFVRNLRSMQTPAFRREGRDIPIVKTIASQKQGIEEVARLIA
                     SDREKELSSGKRYRLLAEQAWLLIQHQRMKGIDRRELAATIEALSKKGPFNLYQFVKN
                     FPT"
BASE COUNT          425 a          524 c          615 g          470 t
ORIGIN      
        1 agcggcagcg atgattcggt ccggatcgtt cccaggaaac agatgaccct ggaagagtgc
       61 atggaatata tccagcagga cgagtgtatc gaagtaacgc ccaagaagat ccggctccgt
      121 aagagcatcc tggacgagga agagcggaag cgcttccaga agatgctgaa gaccgaagag
      181 gttggcggat aagtcgtatc tttatggtct aaacggccct ttatgcgcag gagcaatctt
      241 gtattcacct tcatcgtatg cctgctgctg acggcggcgg cactgcccgt aaaggcgcag
      301 tgttcgatct gtacgcgtac ggcccagcaa ttgggtgagc ggcccgcccg ggcgctgaac
      361 aagggcatta tctatctggc tgttactccc cttgccattt ttggcgtcat cgggtatcgt
      421 tggtggaaga gtgaacaaca tgactgaatc gcgtcttgtc tccgtcgttc tgggcaccta
      481 taatggagaa gcctatctgc gggagcaatt ggattccgtc ctctcacaaa cgtattccca
      541 tctcgagatc atcacgatcg atgacggcag caccgatggg acggtcggca tcttacgtga
      601 atacgccgcc cgggataccc ggatcagggt catcgtcaac gagcgcaatc ttgggtttat
      661 ccgcaatttc gaaaagggtt gttccctttc gacgggccat tggatctccc tctgcgacca
      721 ggatgattac tggatgccgg ataaagttga aaaaatggtc aatgctatcg gggaataccc
      781 gatggtctat tgtgactctc tcctttgcga ccaggacctg cagcccttcg ggaacaggat
      841 ctccgacctg gtccattacc agtcttttga cgacccccgt cagctctgtg ttttttcccg
      901 gatgtatggt catgccacgc tgatcacccg tgggcttttt gacaaggcgc ggccctttcg
      961 cgaggaagtt ccccacgatg ggtggctggc ctatcatgct accctgtatg gtggggtttg
     1021 ggatctgccc gaggtgcttg tcaaataccg tcagcatgcc gggaatgtct ttggggtcgt
     1081 gggaaggaag aagaaaaagc ccagtcgcga agaaaaggcc gggaaaagaa aaaaagaact
     1141 cgcgcgtatc cgtgtccgga tgcaggcgta ttgtgatgcc tgtcccgaga cgctggtgcc
     1201 gcaaaagaaa ctgctccggg cgcttgtccg cagctatcgc gacttctcgc cggggaacga
     1261 tatccggcgt gtcctcctct tttttgccaa ttacaaacgc ctgctggtgg tgaagaaata
     1321 ttccacggga aggaaatggc ttttttgcct gaagatgctg gtcaagatca agtagggaag
     1381 ttttttacga actggtaaag attgaacggc ccttttttcg agagggcctc gatggtggct
     1441 gccagctccc ggcggtcgat ccctttcatg cgctggtgtt ggatcaggag ccaggcttgt
     1501 tcggcaagta agcggtaccg tttgcccgag gagagctctt tttccctgtc gctggcgatc
     1561 agccgcgcta cttcttcgat gccctgtttt tgggacgcga tggtctttac gatggggatg
     1621 tcccggcctt cgcggcggaa ggcgggggtc tgcatggagc gcaggttgcg gacgaagaga
     1681 tcggcgtcgg gccggtcact cttattgacg acaaagatat cggccacctc gagcagtcct
     1741 gccttcatgg attggatatc gtcgccgccc tcgggtgcga gtacgaccag ggtggtgtcg
     1801 gcgagaccgg cgatctcgat ctcgttctgt ccgacgccga ccgtttcgat caggatatga
     1861 tcgaagggcg cctgttttaa cagatcggtg atctcgatga tccgggggtg cagcccaccg
     1921 agcgagcccc gggtagcgag gctgcggata aagacagccg gatggttata ccagtcgctc
     1981 atccggattc ggtcgccgag cagggcgcca tagtacagcg aagaagaggg gtcc
//
LOCUS       DATGFG010000927         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_52311, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000927 DATGFG010000000
VERSION     DATGFG010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52311"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VKQ52_17530"
     CDS             complement(<1..272)
                     /locus_tag="VKQ52_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89063.1"
                     /translation="MEKPIKKAVWKNIYRLILPYRKKFLWVVTLGLLSTGVGLIEPLI
                     YREAINDVAGLFVRQARENAQKDLGVDADEEESIRSMMRSELPDSA"
     gene            384..1289
                     /locus_tag="VKQ52_17535"
     CDS             384..1289
                     /locus_tag="VKQ52_17535"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLZ89064.1"
                     /translation="MGYSRRTFLKNSALAVAAAGFLPGEPFANAYKREMVGVQLYSVR
                     DSMAKDPSGSLKKLADMGYKYVEHAGYREGKFYGYGAKEFKALLDGYGLKMHSGHTHL
                     GPGDWDEGKKDFSDGWKRLVGDAAIAGQKYVINPSMDESLRKNYDDLLRFLEVFNKAG
                     ELCKSNGMKFGYHNHDFEFSTVLNNQKMFDIIMQHTDPALVAQQLDMGNLYNGGAIAI
                     AIVMQYPDRFELMHVKDEIKATGGSEKYESTILGQGIVNTKQVCDQGRRSGTKYFIIE
                     QESYQGKDPFDSCKEDLEVMKKWGY"
     gene            complement(1283..>2886)
                     /locus_tag="VKQ52_17540"
     CDS             complement(1283..>2886)
                     /locus_tag="VKQ52_17540"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLZ89065.1"
                     /translation="MKHYLAYSDSRTGKDRTPIYLPEIEMREYYLPQFRAAIKAGAST
                     LMVNSSEINGTPVHASQYLLTDVLRKELGFQGVVVTDWEDIIRLHTRHNVAASPRAAV
                     VMAINAGIDMSMVPSDFSFFILLKEAVQKGEVPLSRIDEAVRRILLLKAKLGLFDNPY
                     PEAAAAGNFGRPEYQALALQAAQEAMTLLKNDDGVLPLNTGARILVAGPAARSISALN
                     GCWSYTWQGKDERWYPANSKTIYDALRDKFGADRVTTTALADFTSAANYDTTALKTAA
                     AGVDAIVLCLGENAYAESPGTIPDLALPEEQKALARTAAATGKPLVIVLTEGRPRFIT
                     DIAPLAKGILMAYWSGRKTAEAISDVLSGDYNPSGRLPFSYPRSMGEMVLYDRKPTED
                     IREIFNSDMTMDGYRPLFPFGWGLSYTPFDYSDLRLSSPGFSAAGKLTISVTVKNAGN
                     RDGKHTVELYTREHYASITPNMRRLRAFKKIFLKAGGSQTVSFTLDRDDLAFVNARLK
                     TVTEPGEFDVYVGELKATFTYHAAQ"
BASE COUNT          623 a          797 c          838 g          628 t
ORIGIN      
        1 tgggcggagt ccggcagttc actccgcatc atcgagcgga tgctttcctc ttcgtccgca
       61 tccacaccga gatccttctg agcgttctct cttgcctgcc gtacaaagag ccccgcgaca
      121 tcattgatgg cttcccggta aatgagtggt tcgatcagcc cgacacccgt actcagcagg
      181 cccagggtca ccacccagag aaatttcttc cggtagggga gtatgaggcg atagatattc
      241 ttccagaccg ctttttttat tggtttttcc atagtatgcg gccgctaaga tcggataaaa
      301 atttatttgg cacccgcagc aaaaaaaatt acattcgtcg ggaacattcc gttagtcttg
      361 ccataacaaa aatatcttcg aacatgggct actcgcgcag aacattcctt aagaacagcg
      421 ccctggctgt ggccgctgcc ggcttccttc ccggggaacc ctttgccaat gcatataaga
      481 gagaaatggt cggtgtacaa ttgtactccg tccgggacag catggcgaag gatccgtccg
      541 gcagcctgaa gaaactggcc gacatgggct ataaatatgt cgagcatgca ggctacaggg
      601 aggggaaatt ctacggctat ggggcgaagg agttcaaggc cctgctcgac ggctacgggt
      661 tgaagatgca cagcggtcac acccacctcg gccccggcga ttgggatgaa gggaagaagg
      721 acttttccga cggctggaag cgacttgtcg gggatgcggc catcgccggg caaaaatatg
      781 ttatcaaccc ctcgatggac gagagcctgc ggaagaacta tgacgacctg ctacggttcc
      841 tggaggtctt taacaaagcc ggcgaactgt gcaagtccaa tgggatgaaa ttcggctatc
      901 acaaccatga cttcgaattc agcaccgtgc tcaacaatca gaagatgttc gatatcatca
      961 tgcagcatac cgatcccgcg cttgttgccc agcagctcga catggggaat ctttataacg
     1021 gcggggcgat cgccatcgcc atcgtgatgc agtatcccga tcgttttgaa ctgatgcatg
     1081 tcaaggatga gatcaaggcc accggcggaa gcgaaaaata cgagagcacg atcctcggtc
     1141 agggcattgt caatacaaag caggtctgcg atcagggtcg cagatccggg acaaagtatt
     1201 ttatcatcga gcaggagtct taccagggca aggatccctt cgattcctgc aaggaggacc
     1261 tggaagtcat gaagaaatgg ggttattgag ctgcgtgata ggtaaacgtg gcttttagct
     1321 ccccgacgta tacatcaaat tctccgggct ccgtcaccgt cttcagccgg gcattcacaa
     1381 aggccaggtc gtcccggtcc agcgtaaaac ttaccgtctg cgatcctccg gctttcagga
     1441 agatcttttt gaaggcacgc aacctccgca tattgggagt gatgctggca tagtgttccc
     1501 gggtgtatag ttcgacggta tgcttcccat ccctgttacc ggcattcttt acggtcacgc
     1561 tgatcgtcag tttcccggcg gcgctaaagc cgggtgaact cagccgcaga tcgctgtagt
     1621 cgaatggcgt atagctcagt ccccagccga atgggaacag cggccggtaa ccatccatcg
     1681 tcatatcgga attgaagatc tcccggatgt cttccgtcgg cttccggtca tagagcacca
     1741 tttcacccat gctgcgcgga tagctgaagg gcaggcggcc cgagggattg tagtcgcccg
     1801 acagcacgtc gctgatcgct tccgccgtct ttctcccgct ccagtaggcc atcaggatgc
     1861 cttttgcaag aggggcgata tccgtgataa aacggggccg gccctcggtc agcacgatga
     1921 cgaggggctt tcccgtggcg gcggccgtcc gggcaagggc cttctgttct tccggtagtg
     1981 cgagatcggg tatggtgccc gggctttccg cataggcgtt ctccccaaga caaaggacga
     2041 tcgcgtccac accggcggcc gcggtcttaa gggcggtggt atcgtagttg gccgcactgg
     2101 tgaaatcggc gagggccgta gtcgttaccc gatcggcgcc aaacttatcc cgaagggcgt
     2161 catagatggt cttgctgttc gcagggtacc agcgttcgtc cttgccctgc caggtatagc
     2221 tccagcagcc attgagtgcg ctgatgctcc gggcggcggg acccgccaca aggatgcggg
     2281 cgccggtatt caggggcagt acgccatcgt cgttcttcaa cagggtcatg gcctcctgcg
     2341 cggcctgcaa ggccagtgcc tggtattcgg gccgtccaaa attaccggcg gcggccgcct
     2401 cgggataggg attgtcgaaa agcccgagct tggccttcag cagcaggata cgccggacgg
     2461 cttcatcgat gcggctgagg ggtacttcgc ctttttgcac cgcttccttc agcaggataa
     2521 agaacgagaa atcactgggg accatgctca tgtcgatccc ggcattgatg gccatcacca
     2581 cggccgcccg ggggctggcg gcaacattat gacgggtatg cagccggatg atgtcttccc
     2641 agtcggtgac caccaccccc tgaaaaccca gttccttgcg gaggacatcg gtgagcaggt
     2701 attggctggc atgaaccggt gtgccgttta tctccgacga attgaccatc agggtggagg
     2761 cgccggcctt tatggccgcg cgaaactgcg gcaggtaata ttcccgcatc tcgatctccg
     2821 gcagatagat gggtgtccgg tccttgccgg tacgggagtc ggaataggcc aggtaatgtt
     2881 tcatgc
//
LOCUS       DATGFG010000928         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1649154, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000928 DATGFG010000000
VERSION     DATGFG010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649154"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..2361)
                     /locus_tag="VKQ52_17545"
     CDS             complement(19..2361)
                     /locus_tag="VKQ52_17545"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ89066.1"
                     /translation="MPEYFLKTAARGFLRNKTQSLIHILGLSTGIAACLFIYGYVHNE
                     LTYDAYNTRAGRIVRVTMVLHAPESNMAFATSPTPLAGALQRDIPGIVATTRIEPTEL
                     VIREGTEYSRSADFCFSEPSVFSVLSFSFLEGTPANALSEPNSIVLTRSMEKRYFGHD
                     RALGRTLICNDHPWLVTGVIADRPPNSDLPINALLSKDYTRMTAWMDDLSVYTFALFQ
                     DKPDCTGLKPKLSQIARYSRPELDSAGATGYSLAFEAEPLTGTHFSKAKLQDTAKGNR
                     AFNTIFSWLAAFILLLALLNYINLSTARATERAKEVGVRKAIGARPLQLIRQFLGESF
                     FLLTLAWLLAIALVMAGAPLFNRLLSIHLALTDGPAILFLVLLFPVTGLLAGGYPALV
                     LSRFNPIKALKGETGRQQGTGLRKALTVIQFVIALVMLTGTAIIYSQMQYIRRKDPGM
                     DRSHIACINLPGDSAKRKADTAFCDALRRQSGIRGISVGSGMPTEGIAMGTTIASANG
                     KKRELMCNYFYIDQQFLPLFHMSLAAGRNLADSLSTDRTEAFLVNEAFVKEMGWRSPL
                     GQSIEGFNHKGRIVGVVRNFFYKSIHNTIEPAVLIYNTFPLAAAMVSISPRELPRLKQ
                     LWKQYFPSSPFNYYFMDENFDAQYAKDRMTMLLFNVFTGLALFISFIGLYGLVSLILL
                     QRKKEIGIRKVLGATPTQLLTLLTKHLLLLLAVATAIALPLAGIAGSRWLASYAYHTN
                     LSLWIFLWSPAVILLLTLAVTGSRIFRASLANPVGALRSE"
     gene            complement(2424..2693)
                     /locus_tag="VKQ52_17550"
     CDS             complement(2424..2693)
                     /locus_tag="VKQ52_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89067.1"
                     /translation="MNEQEKSIGATDAGRIKMYQRRTQKGMGRTEKTVHNGAAKNPRL
                     LKACNITRKRDMGGFFFISMKMRLLFNLFPMIKRTNLHSKCGFTN"
     gene            2743..3483
                     /locus_tag="VKQ52_17555"
     CDS             2743..3483
                     /locus_tag="VKQ52_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89068.1"
                     /translation="MEQQQFKIIIADESHQDFARIICDEMESSAMARGTGIAKRSPDY
                     IQQKMREGKAVIAFTEDGVWAGFCYIETWSHGEYVANSGLIVSPPFRKSGLAKQIKRK
                     IFELSRKTYPDAKIFGLTTGLAVMKINSEIGYEPVTYSELTQDDAFWAGCKSCVNYEI
                     LMSKERKNCMCTAMLYDPKDHYEPEETTQDFKKHSKLYERFMRIKQWKLLAPFLKHHG
                     EDNGSPVGKDGNGGGGGSRLKSFFHFFF"
     gene            3508..>4218
                     /locus_tag="VKQ52_17560"
     CDS             3508..>4218
                     /locus_tag="VKQ52_17560"
                     /inference="COORDINATES: protein motif:HMM:NF012966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HLZ89069.1"
                     /translation="MSNKVVLAYSGGLDTTYCAVYLNKVKHLEIHALTVNTGGFSQAE
                     IDQIAARAKALGVASFECVDVTREYYESCIKYLIFGNVLKNATYPLSVSAERMVQAIA
                     VANHVKKVGASYVAHGSTGAGNDQVRFDMVFQSMLPGVEIITPIRDLRLSREEEIAFL
                     KENGVEMNVEKAKYSINKGLWGTSVGGKETLTSKEYLPEDAWPTPVTGTGVHSLVLEF
                     EKGELRGVDGKSFTNPVEA"
BASE COUNT          905 a          957 c         1416 g          940 t
ORIGIN      
        1 caggggccgc ttcgcgaatc attcgctgcg caatgccccg acggggttgg ccaggcttgc
       61 cctgaagatg cgcgagccgg tgaccgccag ggtcaacagc aggatgacgg cgggagacca
      121 aagaaaaatc catagagaca agtttgtgtg ataggcgtag gacgccagcc agcggctgcc
      181 ggcgatacct gccagtggca gggcgattgc cgttgcgacg gccagcagaa gaagcaggtg
      241 ttttgtcagc agcgtgagga gttgggtggg tgttgcgccg agcaccttgc ggatgccgat
      301 ctcctttttc cgttggagga ggatcaggga taccagacca tagagaccga taaaggaaat
      361 gaagagcgcc aggcctgtaa atacattgaa caggagcatg gtcattctgt ccttcgcgta
      421 ttgggcgtcg aagttctcgt ccataaaata gtagttaaag ggggaggaag ggaaatactg
      481 cttccagagt tgttttagcc ggggaagttc ccgcggggag atgctgacca ttgcggcggc
      541 aagggggaag gtattgtaga tgaggacggc gggttcgatc gtattatgga tggacttgta
      601 gaagaagttc cttaccacgc ctactattct gcccttgtgg ttaaagcctt ctatcgactg
      661 accgaggggc gagcgccagc ccatttcctt tacgaaggcc tcgttgacga ggaaggcttc
      721 ggtcctgtcg gtggataggc tgtcggccag gttacggccc gcggccaggg acatatggaa
      781 gaggggaagg aattgctgat cgatgtaaaa gtagttgcac atcagttcgc gtttcttacc
      841 gttggcactt gcgatggtgg tgcccatggc gatgccttcc gtgggcattc cgctgccgac
      901 cgagatgccg cggatgccgg attggcggcg aagggcgtcg cagaaggcgg tgtcggcttt
      961 ccgtttggcg gagtcgccgg ggaggttgat acaggcgatg tggcttcggt ccatgccggg
     1021 gtctttgcgc cggatgtatt gcatctggct gtagatgatg gctgtgccgg tgagcatgac
     1081 cagggcgatg acgaactgga taacggtgag ggcttttcgc aggccggtgc cctgttgccg
     1141 gccggtctcc ccttttagcg cctttatggg gttgaagcgg gagaggacga gggcgggata
     1201 gccgccggcc agcagcccgg tgaccgggaa gagcaggacc aggaagagga tcgccggtcc
     1261 gtcggtgagg gcgaggtgga tggagagcag gcggttgaag agcggggcgc cggccatgac
     1321 gagggcgatg gcgagcagcc aggccagggt caggaggaag aaggattcgc ccaggaattg
     1381 ccggatcagc tggaggggtc ttgccccgat ggctttgcgg acgcctactt cttttgcccg
     1441 ttcggttgcg cgggcggtgg acaggttgat atagttgagc agggcgagga ggaggataaa
     1501 ggccgccagc caggagaaga tggtgttgaa ggcgcggttg ccctttgcgg tgtcctggag
     1561 tttggccttg ctgaaatggg tcccggtgag gggttccgct tcgaaggcga ggctgtatcc
     1621 ggtggcgccc gcggagtcga gttccgggcg ggagtaccgg gcgatctgcg acagttttgg
     1681 cttgagcccg gtgcagtcgg gcttatcctg gaagagggcg aaggtataga ccgagagatc
     1741 gtccatccag gctgtcatcc gggtgtagtc ctttgacagg agtgcgttta tggggagatc
     1801 ggaattgggt gggcgatcgg cgatgacgcc ggtgacgagc cagggatggt cgttacagat
     1861 cagggttcgc ccgagggccc ggtcgtggcc gaaataccgt ttctccatgc tgcgggtgag
     1921 tacgatggag ttgggctccg agagggcgtt tgcgggtgtg ccttcgagga aagaaaagct
     1981 gagaacggag aagacggagg gttcggagaa gcagaaatcc gccgatctgc tgtattccgt
     2041 tccttcgcgg atgacgagtt ccgtgggttc gatgcgggtt gtcgcgacga tgccggggat
     2101 gtcccgttgg agggcgccgg ccaggggtgt cgggctggtg gcgaaggcca tgttggattc
     2161 gggagcgtgg agcaccatcg ttaccctgac gatgcggccg gcccgggtat tataggcgtc
     2221 gtaggtgagt tcgttgtgga catagccgta gataaagaga caggcggcga tgccggtgga
     2281 gagaccgagg atatggataa gcgattgagt cttattccgg aggaagccgc gggcagctgt
     2341 tttgaggaag tattcaggca taccttttga atgcaggata ttgccgccgg gatgcccggg
     2401 cttcggtaaa atttacataa aatttaatta gtaaatccgc atttactatg taaatttgtc
     2461 ctctttatca ttggaaaaag attaaacaac aatctcatct tcattgaaat gaaaaaaaat
     2521 ccacccatat cacgcttcct ggtgatattg cacgccttca ataagcgtgg gttttttgct
     2581 gcgccgttgt gcacagtttt ttccgttcgc cccattccct tttgggtacg acgttgatac
     2641 atttttatcc ttccggcatc tgtcgccccg atgctttttt cctgttcatt cattccagtc
     2701 ttttcaagct tttgttaaag ggataaatta tccacgcaaa tcatggaaca acaacaattt
     2761 aaaataatca ttgccgacga gtcccaccag gatttcgcgc ggatcatctg tgacgaaatg
     2821 gagtcttcgg cgatggcccg gggtaccggg atcgccaagc ggagtccgga ttatatccag
     2881 cagaagatgc gtgagggcaa ggccgttatc gcgttcaccg aggacggggt atgggccggg
     2941 ttttgttata tcgagacctg gagtcatggg gaatatgtcg ccaactccgg gcttatcgtt
     3001 tccccgccgt tccggaagag cgggctggcg aagcagatca agcggaagat ctttgaattg
     3061 agccggaaga cctatccgga cgccaagatc tttggtctga cgacgggtct ggccgttatg
     3121 aagatcaact ccgaaatcgg gtatgagccg gttacctatt ccgagcttac gcaggatgat
     3181 gccttttggg ccggatgcaa gagttgtgtc aactatgaga tcctgatgag caaggagcgg
     3241 aagaactgta tgtgtacggc catgttgtat gaccccaagg accattacga gccagaggag
     3301 accacgcagg attttaagaa gcattccaaa ttatacgagc gattcatgcg catcaagcag
     3361 tggaagctgc tggcgccctt cctcaagcat cacggcgaag acaacggcag cccggtgggc
     3421 aaggatggta atggcggggg cggcggcagc cggctcaaat cgttcttcca tttcttcttt
     3481 taatctctaa aaatccattt ttcagccatg agcaataaag tcgtcttagc ctatagcggc
     3541 ggattggaca caacctattg tgctgtttat ctcaataagg tgaagcatct cgagatccat
     3601 gcccttacgg tgaatacggg tgggttcagc caggcggaga tcgaccagat cgccgcgcgg
     3661 gcgaaggccc tgggggttgc ctccttcgaa tgtgtggatg tgaccaggga gtactacgaa
     3721 agctgtatca aatacctcat cttcgggaat gtgttgaaga atgccaccta cccgcttagt
     3781 gtcagtgcgg agcgaatggt gcaggcgatc gccgtggcca atcatgtgaa gaaggtgggc
     3841 gcgtcctatg tcgcgcatgg cagtacgggt gcggggaacg accaggtgcg gtttgacatg
     3901 gtcttccaga gcatgcttcc gggggtggag atcatcacgc cgatacgtga cctgcggctg
     3961 agccgggagg aggagatcgc ctttttgaag gagaatggcg tggagatgaa cgtggagaag
     4021 gcaaaatata gtatcaataa gggtttgtgg ggtacatcgg tcggcgggaa ggaaacgctt
     4081 acctcgaaag agtatttgcc ggaagatgcc tggccgacgc cggtgaccgg gacgggggtc
     4141 cattcgctgg tgctggaatt cgagaagggt gagctgcggg gcgtggatgg caagtccttt
     4201 accaatcctg tggaggct
//
LOCUS       DATGFG010000929         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_579398, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000929 DATGFG010000000
VERSION     DATGFG010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579398"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..>2426)
                     /locus_tag="VKQ52_17565"
     CDS             complement(256..>2426)
                     /locus_tag="VKQ52_17565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HLZ89070.1"
                     /translation="APFFLTVYDLCRWAREQKILYQGRGSAANSTICYCLRITFVNPT
                     KFDLLFERFMSAARNEPPDIDVDFEHERREEVIQYVYRKYGRDRAAIVATVTQLHYKG
                     AVRDVGKAMGLSTDMLDHLSKARAESEGEWMEGKGFAEAGLNPADPLLHRVLELTRQL
                     MGFPRQLGQHTGGFVITQKKLSDLCPVLNARMEDRTNIEWNKDDIDALGIIKVDVLAL
                     GMLTCIRKAFTLAKEHYGLDLTLANIPQDDPAVYEMISHADTIGVFQIESRAQQAMLP
                     RLRPNCFYDLVIEVAIVRPGPIQGDMVHPYLRRRTGLEPVEFPSKALEDILGRTLGVP
                     LFQEQAMKIAIVAAGFTPTEADELRRSMATFKAHGLISRFREKLVSGMVKNGYTEEYA
                     HRIFKQLEGFGSYGFPESHAVSFALLAYISAWLKRRLHDVFTCALLNSQPMGFYHSDQ
                     IVTDAKRHGVEVRPIDVNHSSWDNTLEERVGQYCMLRLGFRQVKGLSEEDVQPLLAGR
                     IGRYTTMNALLDAGVSVGALELLAGADAFGSLGLDRRQALWEVSALKDNPIGVFAGQP
                     SETVFEGRIELPGMPLPEQVTADYAATSLSLKAHPVSFIRPTFDQLGIWPVKRLAEGR
                     DGDAVKVAGRVRVRQHPGTAKGVLFITIEDETGDANLVVFPNVFEAYRKPILQSTLLM
                     VEGKLQVEGEVIHVIVRHCYNLSPLFRTPEKLYANARNFR"
BASE COUNT          523 a          827 c          599 g          477 t
ORIGIN      
        1 gtccaatgtc caatgttccg aaaaatccgt acccaggtcc taagtccaaa atccaatgtc
       61 caatgtccga agaacccgta cccaatgtcc tgaaccctat gtcgaaaacc ctgatgtcct
      121 aaattgcaag ccgttgctga acgtccgact tcctgccccg ttttaaccca atgcctccgt
      181 ggaaacccaa ttttgcctct acccaagggt aatgaaatga actcagcccg ccgaacccgg
      241 cgggcttttt caatcctacc ggaaattgcg cgcattcgca tacagctttt ccggggtccg
      301 gaacaggggg gacaggttat agcaatgccg cacgatcaca tggatcacct ccccctccac
      361 ctgcagcttc ccttccacca ttaacaaagt agactgtagg atcggcttcc ggtacgcctc
      421 gaacacattc gggaagacaa cgaggttggc gtcccccgtc tcgtcctcga tcgtgataaa
      481 aagcaccccc ttcgccgtac ccggatgctg ccgcacccgc acccggcccg ccaccttcac
      541 cgcatccccg tcgcggccct cggcaagccg cttgacgggc cagatcccca gctggtcgaa
      601 ggtcggccgg ataaaactca caggatgagc ctttagtgaa agggaggtag ccgcataatc
      661 cgccgtcacc tgctctggca gcggcatccc gggaagctcg atcctgcctt cgaaaacggt
      721 ctccgaaggc tgcccggcga acaccccgat cgggttatct ttcaacgccg acacctccca
      781 aagcgcctgg cgccggtcga ggccaagcga cccaaacgca tccgcgcccg ccagcaattc
      841 cagcgcccct accgataccc ccgcatccag caaggcattc atcgtcgtat agcgcccgat
      901 ccggcccgcc agcagcggtt ggacatcctc ctcgctcaat ccctttacct gccggaaccc
      961 caatcgcagc atacaatact ggcccacccg ttcctcgagc gtgttatccc aggatgaatg
     1021 attgacatct atcggccgca cctccacccc atgccgcttc gcgtcggtga cgatctgatc
     1081 ggaatgataa aagcccatcg gctggctgtt gagcagcgcg caggtgaaca catcatgcag
     1141 ccgccgcttc agccaggccg agatataggc cagcagggcg aagctcacgg catgactctc
     1201 ggggaacccg taagagccaa aaccctcgag ctgtttaaag atgcgatgcg catactcctc
     1261 ggtatagccg ttcttcacca tcccgctgac cagcttctcg cggaaccggc tgatcaatcc
     1321 atgcgccttg aacgtggcca tgctccggcg cagctcatcg gcttcggtgg gcgtgaatcc
     1381 cgcggccaca atggcgatct tcatcgcctg ctcctggaac agcgggaccc ccagcgtccg
     1441 gccaaggata tcttccagcg ccttcgaagg gaattccacc ggctccaggc cggtccgacg
     1501 tcgaagatag ggatgcacca tatccccctg gatcggcccc ggccgcacaa tggccacttc
     1561 gatgacaaga tcataaaagc aattgggccg cagtctcgga agcatcgcct gctgcgcccg
     1621 gctctcgatc tggaaaacgc cgatcgtatc cgcatggctg atcatctcat acaccgcggg
     1681 atcgtcctgc gggatattgg ccagcgtcag gtcgaggcca taatgttctt tggcaagcgt
     1741 aaaggccttg cggatacagg taagcatccc cagcgccaac acatccacct tgatgatgcc
     1801 aagggcgtcg atatcgtcct tgttccattc gatattcgtc cggtcttcca tccgcgcatt
     1861 caacacgggg cacagatcgg acaacttctt ctgcgtgatc acaaaaccac cggtatgctg
     1921 tcccagctgc cgcgggaatc ccatcagctg ccgcgtaagc tccagcaccc ggtgcagcag
     1981 cgggtctgcg ggattcaaac cggcctccgc aaaccctttt ccttccatcc actctccttc
     2041 ggactcggcc ctcgccttcg acaggtgatc gagcatgtcg gtcgaaaggc ccattgcctt
     2101 ccccacgtcg cgcaccgccc ccttataatg caactgcgtc accgtcgcca cgatcgccgc
     2161 ccggtcccgc ccatacttcc ggtaaacata ttggatcact tcttctcttc gttcgtgctc
     2221 gaagtcgaca tcgatatccg gcggttcgtt ccgcgctgcc gacatgaacc gttcgaaaag
     2281 cagatcgaat ttggtcgggt tgacaaaggt gatccgcaga caataacaga tcgtggaatt
     2341 ggccgccgaa ccccgcccct ggtagaggat cttttgttcg cgggcccatc ggcaaagatc
     2401 atacaccgtc aggaagaacg gcgcat
//
LOCUS       DATGFG010000930         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1546150, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000930 DATGFG010000000
VERSION     DATGFG010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546150"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VKQ52_17570"
     CDS             complement(<1..797)
                     /locus_tag="VKQ52_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89071.1"
                     /translation="MKYRGILLFTALLAGSLAKAQYNPDKVNKKAAQLYSKALELAQG
                     DDFKGGIAALQQAVGIDKNFEEAFLSMAGMYGELKDYQNAIDNYEKARAIDSNFFKDY
                     NLPYSINLAGKGDFQKALDAVNVFLTVTDLNETSRKAGEYRQRCYRFALDYAASHPGA
                     GYKFEPHNLGDSINTSVSEYYPTITIDGNKLIYTRRVNNFNEDFYESDKTNGVWQKSI
                     SLPGNINTNQNEGAQNISQDGQWLIFTGCNFPDGHGSCDLYISYLTA"
     gene            complement(812..1423)
                     /locus_tag="VKQ52_17575"
     CDS             complement(812..1423)
                     /locus_tag="VKQ52_17575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HLZ89072.1"
                     /translation="MNKIAVGLPVMESFYTLQGEGFHQGRAAFFIRLGGCDVGCVWCD
                     VKESWDADNYPRLSVEELVATASAYPGRLAVITGGEPLMHDCTELTRELHRAGFATHI
                     ETSGAWPASGEWDWICLSPKKFKAPLPEIVPRANELKVVIYNKTDFSWAEQWAALTSP
                     GCRLFLQPEWSKSATVTPLIIDYIKAHPQWGFSLQLHKYIHVP"
     gene            complement(1416..>2312)
                     /gene="folD"
                     /locus_tag="VKQ52_17580"
     CDS             complement(1416..>2312)
                     /gene="folD"
                     /locus_tag="VKQ52_17580"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF008058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HLZ89073.1"
                     /translation="YLCGMELLDGKIASKAIKDTLTLKVAQLIAEGKKIPHLAAVLIG
                     NNGASETYVGAKVKACAEIGFRSTLVRLDDSISENRLLTVIEELNNDPEVDGILVQLP
                     LPKHISDEKVIDAIHPSKDVDGFHPENVGRLVQGLPAYIPATPHGILLLLEHYKIDTR
                     GMHAVVVGRSNIVGRPMSILLSSNSNPGNCTVTLCHSQTRDIAGLCLQADIIVAALGR
                     PEFVRASMVKKGAIVIDVGITRVPDASKKSGFALKGDVKFDEVAPLCSYITPVPGGVG
                     PMTIAALMKNTLTACEERNGNE"
BASE COUNT          495 a          629 c          618 g          570 t
ORIGIN      
        1 tcggcggtga gataggagat ataaaggtcg cagcttccat gaccatcggg aaagttgcat
       61 ccggtgaaga tcagccactg accatcctgc gaaatgttct gtgcgccttc gttctgattg
      121 gtattgatat tacccggaag actgatggac ttctgccaga ccccgttcgt tttatccgac
      181 tcgtagaagt cttcgttgaa attattgacc cgccgggtat agatcagttt attcccgtcg
      241 atggtaatgg tggggtagta ttccgagacg gaagtgttga tgctgtcgcc caggttgtgc
      301 ggctcgaatt tataacccgc ccccgggtga ctggcggcat agtcgagggc aaaacggtag
      361 catcgttgcc tgtattcacc cgctttcctg ctggtctcat tgagatcggt gacggtgaga
      421 aagacattca ccgcgtccaa cgccttttga aaatcgcctt tcccggccag gttgatcgaa
      481 taaggcagat tatagtcttt aaagaaattg ctgtcgatgg ccctggcttt ttcgtagttg
      541 tcgatcgcgt tctggtagtc cttcagttcc ccatacatcc ccgccatcga gaggaaggct
      601 tcctcgaaat tcttatcgat gccgacggcc tgttggaggg ccgcgatccc ccctttaaaa
      661 tcatctccct gagccagttc aagggccttg gaatacaact gggccgcctt tttattcact
      721 ttgtcgggat tgtactgggc ctttgccaga gatccggcca gcaatgcggt aaaaagaagg
      781 atgcctcggt atttcatata tggatattta attatggcac atggatatat ttgtgcagct
      841 gcagggagaa gccccattgg ggatgcgcct tgatataatc gatgatcaac ggggtgacgg
      901 tggcggactt gctccattcc ggctgcagga aaagccggca tccgggggag gtcagggcgg
      961 cccattgttc agcccaggaa aagtccgtct tgttatagat gacgactttt agctcattgg
     1021 cccgggggac gatctccggc aacggggcct tgaatttctt cggggaaaga cagatccagt
     1081 cccattcacc gcttgccggc caggccccgg aggtctcgat atgcgtggca aaacccgcac
     1141 gatgtaattc ccgggtcaat tcggtacagt cgtgcatcag cggctcgccg ccggtgatga
     1201 ccgccagacg gccggggtac gcactcgccg tggcaaccaa ttcttccacc gatagccggg
     1261 gatagttgtc cgcgtcccag ctttccttta catcgcacca tacacagccc acatcacagc
     1321 cgccaagccg gatgaagaaa gctgcccttc cctggtgaaa cccttccccc tggagggtgt
     1381 aaaaggattc catgacgggt agccccactg ctatcttatt catttccatt tctttcttca
     1441 caggctgtaa gcgtattttt catcagggcg gcaatggtca tgggaccgac gccgcccgga
     1501 acaggggtaa tataactgca aaggggcgca acctcatcga atttgacatc tcccttcagg
     1561 gcaaatccgc tctttttcga ggcatccggc acccgggtaa ttcctacatc tatgacgata
     1621 gcgcccttct ttaccatcga agccctgaca aactccggcc ggcccagggc cgccacgatg
     1681 atatccgcct gcagacagag cccggcaata tcccgggttt gggaatgaca aagggttacc
     1741 gtgcaattgc ccgggttgct gttgctgctg agcaggatgc tcatgggtcg cccgacgata
     1801 ttgctccgtc cgaccacgac ggcgtgcatc cccctggtgt cgatcttgta atgctccagc
     1861 aggaggagga tgccatgggg agtggccggg atataggcgg gaagaccctg taccagcctg
     1921 cctacattct cgggatggaa gccatctaca tccttggaag gatgaatggc atcgatcact
     1981 ttctcgtcgg aaatatgttt gggcagaggt aactgaacca ggatgccgtc gacttcagga
     2041 tcgttgttca attcttcgat cacggtaagc agccggttct cgctgatgct gtcatccagc
     2101 cggacgaggg tcgaccggaa cccgatctcg gcgcaggcct tgactttcgc ccctacatag
     2161 gtctcgctgg ccccgttatt cccgatcagc accgccgcca gatggggtat cttctttcct
     2221 tccgctatta actgtgcaac tttaagagta agagtatcct ttatcgcctt tgatgcaatt
     2281 tttccatcta ataattccat gccgcaaagg ta
//
LOCUS       DATGFG010000931         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1004565, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000931 DATGFG010000000
VERSION     DATGFG010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004565"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VKQ52_17585"
     CDS             complement(<1..855)
                     /locus_tag="VKQ52_17585"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ89074.1"
                     /translation="MQENPYHQDREEMKELLKQYSDLRSGRNHSFLEEEAFERIIDYF
                     DDTEDLTQAIEAVELGIEQYPYSSALLVKKADLMIATRRYYEALTILEQVELLDGSDI
                     NLYILKTDAYLALDQPQKAAALLESALLHFEGDERVELLFELADVYDDYEEFDKIFDC
                     LKLILEQDPANEEALYKICFWTDFTGRNEESIRLHQQIIDEKPYNELAWFNLAAAFQG
                     LKLYEKAVDAYKYALAIDEKFDYAYRNMGDAYIRLRKYRDAIESLEKVLELSRPEDVI
                     YEAIGHCYD"
     gene            1160..1552
                     /locus_tag="VKQ52_17590"
     CDS             1160..1552
                     /locus_tag="VKQ52_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89075.1"
                     /translation="MLTPTTIEWRKLMRKMLVSAVMVAVVGLAFASKGGGGDKNKANS
                     PLKTNFTPIRTTNGFTLKAGPSFSGSILLGTEKTANYVSFNTLVTYEKGNSIYIMPYR
                     YKVNSSLFQHNSNNSLQLLDLHINVGHK"
     gene            complement(1554..2219)
                     /locus_tag="VKQ52_17595"
     CDS             complement(1554..2219)
                     /locus_tag="VKQ52_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89076.1"
                     /translation="MLQYRNPLCLLFLCILLQSAARAQKRISELTLVYDYSVTIEGGN
                     KLEGVGNATHTIYIKGNKSRSEMTNALFSSTSIYDANTGFGVVLREVSGQKLLIRMNP
                     DNWSERNRMYEGIVFKNTSETKIIAGYNCIKATGQTTAGATVTVFYTRELIPDNKQYD
                     PIFRALEGLPLEYELTNGNVKISYRFSKMSLNPVPASKFDIPNSGYREMNYEESKKMN
                     IGG"
     gene            complement(2269..>3323)
                     /locus_tag="VKQ52_17600"
     CDS             complement(2269..>3323)
                     /locus_tag="VKQ52_17600"
                     /inference="COORDINATES: protein motif:HMM:NF014186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HLZ89077.1"
                     /translation="QQFFNSTLIAKNGFGAGNVHWQRESADSSNVVARGDIALQAKIP
                     AAAAATMPFRLYYGPNDFSILDKEAPGMTHIIDLGRGIYSFVRPVNVYIIMPVFDFFR
                     QHIASFGIAILLLTLFIRLFTSPLVYSSYLSGAKMKALRPEIETLRKKIGDDQQQMGM
                     EQMKLFREAGVNPLGGCIPALLQIPIFFALYSFFSSNIGLRGESFLWSHDLSMYDSVL
                     SFGFSIPGYGDHVSLFALLAVVTSFLISLYGMSMTPDQSNPAMKYMPYIMPVFLLVFF
                     NRLASALTWYYTVSNLITLALQFVIQNYIIDHDKILSKLQENRAKPKTKSKWQERLEQ
                     MQEAQKEAQKRAPGRK"
BASE COUNT          848 a          948 c          751 g          776 t
ORIGIN      
        1 atcatagcaa tgcccaatcg cctcatagat cacatcctcc ggcctcgata actccagcac
       61 cttctccagc gactcgatcg catcccggta cttccggagc cggatatacg catcccccat
      121 attccggtag gcataatcga acttctcatc gatggccagg gcatacttat atgcatccac
      181 cgccttctca tacaacttga gcccctggaa cgccgccgcc aggttgaacc aggccagctc
      241 gttatacggc ttctcatcga tgatctgctg atgcagccgg atactctcct cgttccggcc
      301 cgtaaagtcg gtccagaaac agatcttgta cagtgcctcc tcgttggccg ggtcctgctc
      361 gagaataagc ttcagacaat cgaatatctt gtcgaactcc tcatagtcgt catagacatc
      421 cgccagctcg aacaacaact cgacccgctc gtccccctcg aaatgcaata acgcgctttc
      481 cagcagggcc gccgccttct gtggctggtc cagggccaga tacgcatccg tctttaaaat
      541 gtaaaggttg atatcgctgc catcaaggag ctccacctgc tcgagaatag tgagggcttc
      601 gtaataccgg cgggtagcga tcatcaggtc cgccttcttc accaggaggg ccgaggaata
      661 agggtattgc tctatcccta actcaaccgc ctcgatcgcc tgggtcagat cctccgtgtc
      721 gtcaaaataa tcgataattc tttcgaacgc ttcttcctcg agaaaggaat gattacgccc
      781 acttcttaaa tccgaatact gcttcaacaa ttccttcatt tcttcccgat cctgatggta
      841 tggattttct tgcatgtgtt aaaggttatc gtaagttact gcataaatac attcccgccg
      901 aaattaaatt tctccccgga tgtgaaccat ttcccctctc cacagcggca ctccgacagg
      961 gtaaaatgtt gatcagggcg gcaaccttcc acgcgcattt aacgtatctt ttgctataca
     1021 ccgctaaaaa accgggctat acagacagga aaaaatcact aaattgtaac ttaatcgggt
     1081 tcaagccgtt aaaggtttac taaattttgt gataagcggt taaaaataac ccaatttttt
     1141 ttactacatt tgtaccagaa tgcttacacc gacaacaata gaatggagaa aattgatgcg
     1201 caaaatgctc gtcagcgccg ttatggttgc ggtagtcggt ttggcctttg caagcaaagg
     1261 cggcggcggc gataagaata aagccaacag cccgcttaaa acgaacttta ccccgatccg
     1321 cacgacaaac ggcttcaccc tcaaggccgg tccttccttc tccggcagca ttctcctggg
     1381 caccgaaaag acagctaact acgtatcttt caatacattg gtcacctacg aaaaaggcaa
     1441 tagcatatat atcatgccct atcggtataa ggtgaacagc tccctgttcc agcacaatag
     1501 caacaacagc ctccaattgc tggacctgca catcaacgta ggccataaat agtctacccc
     1561 ccgatattca ttttcttgct ttcttcgtaa ttcatctcgc ggtacccgct gttgggaata
     1621 tcgaattttg acgcgggcac cgggttcagg ctcattttcg agaaccggta ggagatcttt
     1681 acattcccat tggtgagttc atattcaagc ggaagcccct cgagagcccg gaagatagga
     1741 tcatattgct tgttatcggg gataagctcc ctggtgtaga atacggtgac ggtagccccc
     1801 gcggtagtct gcccggtcgc cttgatacag ttgtaaccgg cgatgatctt ggtctcgctg
     1861 gtattcttaa aaacgatgcc ctcatacatc cggttccgct cggaccagtt atcggggttc
     1921 atccggatca acagcttctg cccgctcacc tccctgagaa ccaccccaaa acccgtattg
     1981 gcatcataaa tggacgtgga agagaaaagg gcattggtca tctcgctacg gctcttattg
     2041 cccttgatat aaatggtatg agtggcgttc ccgacgcctt ccagcttatt ccccccctcg
     2101 atggtaacag aatagtcata gaccagggta agctccgata tcctcttctg ggcccgcgcc
     2161 gctgattgaa gaagaataca taggaataac aggcataaag ggttacgata ttgcaacatc
     2221 cgttaaaaat aagaaagacc cctcaaaact ccaaggaaac accccctcct atttcctccc
     2281 cggagcccgc ttctgggcct ccttctgcgc ctcctgcatc tgctcgagcc gctcctgcca
     2341 cttcgacttg gtcttcggct tggccctgtt ctcctgcagc ttcgacagga tcttgtcatg
     2401 gtcgatgata taattctgaa tgacgaactg caacgccagc gtgatcaggt tggagaccgt
     2461 atagtaccag gtcagggccg aagccagcct gttaaagaag acaagcagga acaccggcat
     2521 gatatacggc atatacttca tggccggatt gctctggtcc ggcgtcatgc tcatcccata
     2581 cagcgagatc aggaaactgg tgacaacagc aagaagggca aaaagactca catgatcgcc
     2641 ataacccggg atcgaaaagc caaaactgag aacgctgtca tacatcgaca gatcatggct
     2701 ccacaggaaa ctttcccccc gcagcccgat attcgaacta aaaaaactat acagcgcaaa
     2761 gaagatcggt atctgtaaca gggccgggat acaaccgcca agcgggttga cccccgcctc
     2821 ccggaacaac ttcatctgct ccatccccat ctgctgctgg tcgtcgccga tcttcttccg
     2881 cagggtctcg atctcgggcc gcaatgcctt catcttggcg ccgctgagat aactgctgta
     2941 tacaagcggc gacgtgaaga gccggatgaa aagggtcagc agcaggatgg cgatcccaaa
     3001 gctggcgata tgctgcctga agaaatcgaa cacgggcata atgatataca cattcaccgg
     3061 ccggacaaag gagtaaatac cccgtcccag gtcgatgata tgcgtcatgc ccggcgcttc
     3121 tttatccagg atcgaaaaat cattgggtcc ataatacagc cggaacggca tggtcgcggc
     3181 agccgcggcc gggatcttcg cctgcagcgc gatatccccc ctggcaacga cattggaaga
     3241 atccgccgat tcccgctgcc agtgcacatt gcccgcgcca aatccatttt tggcgatcag
     3301 cgtcgaattg aagaattgct gcg
//
LOCUS       DATGFG010000932         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_163983, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000932 DATGFG010000000
VERSION     DATGFG010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163983"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VKQ52_17605"
     CDS             complement(<1..1310)
                     /locus_tag="VKQ52_17605"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89078.1"
                     /translation="MLRNYFTVALRNLWKNKGFSAINIIGLAAGLGVCLLIVLYVIDE
                     LSYDRYNVKADRIYRIDADIYFNNTQFYAVATPRPLARTLVGEYPQVEQMVRTNYQGD
                     VMVKKGAGFIQDHRLVFADSTLFQVFTIPMIMGNPMTALNEPNTIVIDESAARRYFNS
                     TDVVGKTLELEDHTVCKVTGVIRDMPSASHFHFSFIRPLRDFFNRGDENDWLSNNYTS
                     YILLRQGVSRDFVQSRIDATVDHYLGKQLLDLLHTSTQDLKKAGNHFKYELMPLTDIH
                     LHSNKSYEFEANGNINFVYIFSMVAILILLIACVNFMNLSTARSANRAREVGIRKVAG
                     STKGHLIIQFLMESTLLSFFSLVLALGIALLLLPVFNHLAGKELHAGVLFSGRFLPIL
                     VLLVFLVGVIAGSYPAFYLSSFQPIQVLKGKLASGFRRSWLRSGL"
     gene            1459..1824
                     /locus_tag="VKQ52_17610"
     CDS             1459..1824
                     /locus_tag="VKQ52_17610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ89079.1"
                     /translation="MPDPRKILLIDDDLDHLSICKLILTNRGYNILVLWNTDVLLEVT
                     KDFMPDLIFMDHRMPHRDGIQATKLLKANEATKHIPVVYFSGMEKAEQFAKEAGADGF
                     LPKPFNVDTLLAAINKYLP"
     gene            complement(1848..>2997)
                     /locus_tag="VKQ52_17615"
     CDS             complement(1848..>2997)
                     /locus_tag="VKQ52_17615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ89080.1"
                     /translation="SFIRVLYREEGKVRVLFESPGILGQVATPVRPGVNDTLGLRVAY
                     VRGASGDPPAEIVLAVSNGQLQSNLRLLLSMLLVSSVFSVLISGLVSYILARILMLPL
                     QRIIHAARNINTNKMRDPIPVNGTRDELHELSVTINDMMHRIDESLQQQQNFFGSASH
                     ELKTPLAILRTELEVGLRKPGLEEGVRHLLSNQLEEISRLQEIVNEFLVVSQLRVGSP
                     SIYRRPFDLSVLVVKVYNQLLPLLRQKNLEPVIRFDEEAADFILNADEDKIRIVLLNL
                     LENAAKYGVRDTPITCKVERSATAGELTVEFTNVTSAEKVNTENLQVAFYRSDLIQKG
                     AGLGLWLCNEIIRLHGGRTEISSVMHRFTVRISLSIMEPVAREEVGPL"
BASE COUNT          732 a          928 c          705 g          632 t
ORIGIN      
        1 agcccgctcc gcagccagct cctcctgaac cccgacgcga gctttccctt caacacctgt
       61 atcggctgaa aagaggatag gtagaaggcc gggtaactcc ccgcgatcac ccccaccagg
      121 aataccagca gcacaaggat cggcagaaat cgcccggaga atagcacacc cgcatgcaac
      181 tccttacccg ccaggtggtt gaacactggc aacagtaata acgcgatgcc cagcgccagc
      241 accagcgaaa aaaagctgag cagggtagac tccatcagga actggatgat aagatgcccc
      301 ttcgtactcc ccgccacctt ccggatcccc acctccctgg cccggttcgc actcctcgcc
      361 gtactcaggt tcataaaatt cacacaggcg atcaacagga tcaggatcgc gaccatcgaa
      421 aaaatataga cgaaattgat attgccgttg gcctcgaact cataagattt attcgaatgc
      481 agatggatat ccgtcaacgg catcagctca tatttgaaat ggttgcccgc cttcttcagg
      541 tcctgggtgg aagtatgcag gaggtccagc agttgcttgc caagataatg gtccaccgtc
      601 gcatcgatcc ggctctgcac gaaatccctg ctgacgccct ggcgcaacag gatatagctc
      661 gtataattgt tgctcagcca gtcgttctca tccccacgat taaagaagtc ccgcagcggc
      721 cggataaaac tgaaatggaa atgggacgcc gacggcatat ccctgatcac ccccgtcacc
      781 ttgcaaacgg tatgatcctc cagctccagc gtcttgccga cgacatccgt gctgttgaaa
      841 taccgccttg ctgcgctctc atcgatcacg atcgtattgg gctcgttcag cgccgtcatc
      901 gggttgccca tgatcatcgg gatggtaaac acctggaaca aggtcgagtc cgcaaacacc
      961 aggcgatggt cctggataaa acccgccccc ttcttcacca tcacatcccc ctggtaattc
     1021 gtccggacca tctgctccac ctgcggatat tccccgacca gggtccgggc cagcggccgc
     1081 ggcgtcgcaa ccgcatagaa ctgagtatta ttgaaataga tatccgcatc gatccggtag
     1141 atccggtccg ccttcacatt gtaccggtca tagcttagct cgtcgatgac atacaacaca
     1201 attagcagac acacaccgag ccccgccgcc agaccaatta tattgatcgc ggaaaaaccc
     1261 ttgttcttcc aaaggttacg cagtgccacg gtaaaatagt ttctaagcat ggggaaattt
     1321 ctctacaaat tgaatatcaa catcttgtta ataactctaa tgggtttccg gctgcgaaat
     1381 taatttttct tcatatagat gccggcgcga cttggaaggg gactcagcgc ataaatttac
     1441 gcccaaataa agctcggcat gcctgatccc cggaaaatct tactgattga cgatgacctg
     1501 gaccatttat cgatttgtaa gttgattttg acgaaccggg gatataacat attggtactc
     1561 tggaacacgg acgtgttgtt ggaagtgaca aaggatttta tgcccgatct catcttcatg
     1621 gatcaccgaa tgccccatcg cgacgggatc caggccacca agctactgaa agccaacgag
     1681 gccacaaaac atatcccggt ggtctacttc tccgggatgg aaaaggccga acaattcgca
     1741 aaagaagcag gggccgacgg cttcctgcca aagcccttca acgtcgatac actcctcgcc
     1801 gccatcaaca aatatcttcc ctgatcgccc catccgtcaa tctcccgcta cagggggccc
     1861 acctcctccc gggcaacggg ctccatgatc gacaggctta ttcttaccgt gaaccggtgc
     1921 ataacgctgc tgatctcggt cctgccccca tgcaaccgga tgatctcgtt gcatagccat
     1981 aatccgagcc cggcgccctt ctggataaga tcgctgcggt aaaaagccac ttgcaggttc
     2041 tccgtgttca ccttctcggc gctggtgaca ttcgtgaact cgaccgtcag ctctccggcc
     2101 gttgccgatc gctccacctt acaggtgatt ggcgtatccc gcaccccata tttggccgcg
     2161 ttctccaaca ggttcagcag aacgatcctg atcttgtcct cgtcggcgtt aaggataaaa
     2221 tcggcggcct cctcgtcaaa ccggatcacg ggttccaggt tcttctgccg cagcaatggc
     2281 agcaattgat tatacacctt caccaccagc accgacagat cgaaaggccg gcggtaaata
     2341 gaaggtgacc cgacacgcag ctgactgacc accaggaact cattgactat ttcctgcagc
     2401 cggctgatct cctccaattg attgctcagc aaatgccgga caccttcctc gagacccggc
     2461 ttacgcaacc caacctccag ctccgtccgt aaaatggcca gtggcgtctt cagctcatgg
     2521 gaagcgctgc cgaaaaaatt ctgctgttgc tgcagggact cgtcgatgcg gtgcatcata
     2581 tcattgatcg tgacgctaag ttcatgcagt tcatccctcg tcccgttgac gggtatcgga
     2641 tcccgcatct tattggtatt gatattccgg gcggcatgga tgatccgctg caacggcagc
     2701 atcagtatcc gggcaaggat ataggacaca agccccgaga taagcacact aaatacactg
     2761 ctcaccaaca gcatgctgag cagcagccgc aggttggact gcaactgacc gttgctgacc
     2821 gccagcacga tctcggcggg aggatccccc gacgcgccgc ggacatacgc gacccgcagc
     2881 cccagcgtat cgttcactcc cggccggaca ggcgtggcca cctgccccag gatacccggc
     2941 gactcgaaca acacccgcac cttcccctcc tcccggtaaa gcacccggat aaaacta
//
LOCUS       DATGFG010000933         5480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1035346, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000933 DATGFG010000000
VERSION     DATGFG010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035346"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1007
                     /locus_tag="VKQ52_17620"
     CDS             189..1007
                     /locus_tag="VKQ52_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLZ89081.1"
                     /translation="MKSIVVPVNYTPNSANAARYAADLALSIGADIHLFHTFQVPMSI
                     AEVPMPEPVFDELQNNGLDLLKNLSAELVNRGSGRIRVSTEMQIGTVESALEQFCKEK
                     EPLLVVMGATGGGLENALLGSNTVRAVRHLPYPILVVPEDAHFQPIKNVVVACDREDL
                     FLGLPVATPVLRELSGLPDVRLEVVHVITGEESASEFTEAYNRWKGQVKGMQPELHFV
                     RGAGVEEGLTEHLRGRPADLVLVFPKRHGLLEFHRSHARRLVLHCAVPVMSLHE"
     gene            1035..1877
                     /locus_tag="VKQ52_17625"
     CDS             1035..1877
                     /locus_tag="VKQ52_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLZ89082.1"
                     /translation="MKKILIATDFSVAAQNATDYGVRLAQAVGATVILASAFEEVPVP
                     VTDTPLIAVPEDLRGLVQRQLELEADSFPRERAVSVEVLPCEGPASTAILGAARSMNA
                     ELIIAGMKGSGKSLRKIFGSTVTGLARRTSIPLLIIPEGVSFAPLKAIAMASDVAHET
                     DYGIPVAVRRLADQFHSKIFVIRLFNKQAGEVIEILHQSANLNRTIGAFAPLCEIPAD
                     ATVAEALKDYIAASPIDLLVMKPHPKTLPERWFLRSNTRQMIFQTDVPLLILPGEPHK
                     NEVL"
     gene            1874..2431
                     /locus_tag="VKQ52_17630"
     CDS             1874..2431
                     /locus_tag="VKQ52_17630"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HLZ89083.1"
                     /translation="MSIISDVRTVVRYWWLFLLLGCLLIFFGILVFNSPSAAYAQLSI
                     YFVIAFVINGLFEVGFALGNRLSVRGWGWHLAGGLFDLLAAGVLYFTPVVAATSLPFF
                     AGFWLLFRSIAIIGRCFDLPVVPLERAAMLTVGAAGLAFSFLVLYYPVTGSYMLIFWT
                     GCALIAIGLFYVFLGMHLKRWSNTR"
     gene            complement(2364..3056)
                     /locus_tag="VKQ52_17635"
     CDS             complement(2364..3056)
                     /locus_tag="VKQ52_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ89084.1"
                     /translation="MPTYKQEIHIPAGTRMVPADLVIPGNADCIVIFSHAGANSRLNR
                     LNQAVAEQLQKAGIGTLMPDLLSEDELARSREFDIGLLTQRLSTVTKWLKDRDLFGHY
                     RLGYYATTTGAASALQAAAGLDTTIGAIVCRGGRPDLVREALPEIGSPVLFIVGSLDR
                     YVLQLNREALETLSGEKKLSVVQGATHFFDEEKIKEVAGHAIVWFKAHLTGCCSTSSG
                     ASQGIHRTTRSQ"
     gene            3220..3699
                     /locus_tag="VKQ52_17640"
     CDS             3220..3699
                     /locus_tag="VKQ52_17640"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLZ89085.1"
                     /translation="MIGKLTPPEIEKLLSKEVVGRLGCSDGEMVYVVPISFHYDGEYV
                     YCHTHEGLKIDIMRKHPVVCFEVDRMHNMANWQSVVAHGRFEELTDPILRSQALQRLH
                     ERVLPMVSSETTHLSPDWPFTPLELNKIVGVTFRIRLEEKTGRYEKSHSVAQTFFSI"
     gene            3718..4998
                     /locus_tag="VKQ52_17645"
     CDS             3718..4998
                     /locus_tag="VKQ52_17645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ89086.1"
                     /translation="MNRIGIRAVAALLWAGVFLPAAGQQPERLTIWEAYRVARENYPS
                     SKQRGLIEKTREYTLQNAARGYLPQLSFAGQATMQSDVTNLPIKIPVAGFSMPQYSKD
                     QYKIYGEVDQAVYDGGMIHNQQEAARVAAAIQEKSLDVELHTLFDRVDQLYFGVLLLD
                     AQLRQNDLLRQDIRNGLEKMQAMVDNGTAYKSGVDELQAQLLQAGETQTEENATRKGY
                     LNMLLMLLGKTPGAMVSLTEPPELSLHTTISRPELLLFEFQQRNYDLQDEAAAALIRP
                     RFSLFVQGGYSRPGLNMLSNNFSFYALGGIRMSWNLGGWYTLKRQRALSEIGRRGLDL
                     QRETFLLNTGMQQEQSILEVARYTALLQEDGPIIELRTAVKEAALAQLRQGTLSAHDY
                     LTQVNAEDQSRETRVLHRIQLLRSEYAYQTLIGH"
     gene            5011..>5480
                     /locus_tag="VKQ52_17650"
     CDS             5011..>5480
                     /locus_tag="VKQ52_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ89087.1"
                     /translation="MVNVSAIEKTYVTRKAKTKALDGISFSVDKGEVFGIIGPDGAGK
                     TSLFRILTTLLLPDAGTAMVNGLDTVKDYKEIRKCVGYMPGRFSLYQDLSVEENLEFF
                     AAIFDVDIQQNYALIGDIYRQLEPFKGRKAGQLSGGMKQKLALCCALVHQPLVL"
BASE COUNT         1109 a         1354 c         1775 g         1242 t
ORIGIN      
        1 caatctccgg gatatgctgc gggaggcgat gcactatttg catgtagtct atgaagagcc
       61 aagccattca tagcctttgc cgggcggggg ttatcccggg cgccggctga ccggagtcaa
      121 tatgcggggt gatgggcgtc atggatggag ggtggtggcg ggtgtagctt agcctaaaca
      181 ttaagaccat gaaatccatt gtagtccccg tcaattatac gcctaattct gccaatgccg
      241 cgcgttatgc ggctgatctg gccctgagca tcggggcgga tatccatcta ttccatactt
      301 tccaggtgcc gatgagcatt gcggaggttc ccatgccgga gccggtgttc gatgagttgc
      361 agaacaacgg tctcgatctg ttgaagaacc tttcggccga gctggtcaac cgggggtccg
      421 ggaggatccg ggtgagcacg gagatgcaga tcgggacggt cgagagcgcc ctggagcagt
      481 tttgcaagga gaaggaaccg ttgctggtcg tgatgggcgc cacgggcggt ggcctggaga
      541 atgccctgct cggcagcaac acggtacggg cggtacggca tctaccctac ccgatactgg
      601 tggttccgga ggatgcccat ttccaaccaa taaagaatgt tgttgtcgcc tgtgaccggg
      661 aggacctttt cctcgggctg ccggttgcga cgccggtgtt gcgggaattg agcggcttgc
      721 cggatgtgcg gctggaggtg gtgcatgtca tcaccggaga ggagagcgcg tcggagttta
      781 cagaagcgta taatcgatgg aaagggcagg tgaagggtat gcagcccgag ttgcatttcg
      841 ttcgcggtgc gggtgtggaa gaagggttga cggagcattt acgtggacgc ccggccgacc
      901 ttgtgcttgt atttcccaag agacacggtt tgctggaatt tcaccggagc catgcccggc
      961 ggcttgtcct gcactgtgcg gtgccggtga tgagcctgca tgaatagtct tcccggatgg
     1021 taaaatgaat gatcatgaaa aaaatactta ttgccacgga cttttccgtg gctgcgcaga
     1081 atgcgactga ttatggagtg cggctggcgc aggcagtggg ggcaacggtg atccttgcca
     1141 gtgcgtttga agaggttccg gtaccggtta ccgatacgcc gctcatcgcg gttccggagg
     1201 atctccgggg cctggtgcag cggcagctgg aactggaagc cgactctttt ccccgggaga
     1261 gagcggtgag cgtcgaggtg ctgccctgcg aggggcctgc gtccacggcg atcctggggg
     1321 cggcccggtc gatgaatgcc gagcttatca tcgcggggat gaagggtagt gggaagagtt
     1381 tgcggaagat cttcggcagt acggtcacgg gattggcgag gaggacttcg atcccgctgc
     1441 tgatcattcc cgagggtgtt tcttttgcgc cgctgaaggc cattgccatg gccagtgatg
     1501 tcgcccacga gacagactat gggatcccgg tagccgtccg gcggctggcc gaccagtttc
     1561 attcgaagat cttcgtgatc cggctattca ataaacaggc gggcgaagtg atcgagatcc
     1621 ttcatcagag cgccaatctg aaccggacga tcggggcatt cgcccctctc tgcgagatac
     1681 cggcagacgc gacggttgcc gaggccctga aagactatat cgcggcgagt cctatcgatc
     1741 tgcttgtcat gaaaccgcat ccaaagacat taccggagcg gtggttcctg cgaagcaaca
     1801 cccggcagat gatctttcaa acggatgttc ccctgttgat cctgcccggg gaaccccata
     1861 aaaatgaagt gttatgagca ttatctccga tgtccggacg gtggtccggt actggtggct
     1921 attcctgctg ctgggatgcc tcctgatctt cttcggcatc cttgtattca atagcccctc
     1981 ggcagcgtat gcccaactca gtatctattt cgttatcgct tttgtgatca acgggctgtt
     2041 cgaggtcggt tttgcccttg gcaaccgtct gtcggtgcgg ggctggggct ggcacctggc
     2101 gggcgggttg ttcgatctgc tggccgcggg cgtcttgtac ttcacgcccg tggtggcagc
     2161 gacgtccctg ccgttctttg ccgggttctg gctgttattc cggagtattg ccatcatcgg
     2221 gcgatgtttc gacctgccgg tagtcccgtt ggagcgggcg gcgatgctga ccgttggagc
     2281 agcgggcctg gctttttcct ttctcgtgct ttattatcct gttaccgggt cttatatgct
     2341 gatcttctgg acgggatgcg cgcttattgc gatcgggttg ttctatgtat tccttgggat
     2401 gcacctgaag aggtggagca acacccggtg aggtgggctt tgaaccagac aatggcatgc
     2461 ccggcgactt cttttatctt ttcttcgtca aaaaaatgag tggcgccctg cacgacggaa
     2521 agcttttttt cgccggaaag ggtttccagg gcttccctgt tgagttgcag gacgtagcgg
     2581 tccaggctgc ctacgatgaa caggacgggg gacccgatct cgggcaacgc ttcgcggacc
     2641 aggtcggggc ggccaccgcg gcaaacgatc gccccgatgg tagtatccag gccggcggcg
     2701 gcctgcaggg cggatgccgc acccgtggtg gtagcgtaat atcccaggcg ataatggccg
     2761 aaaagatcgc ggtccttcaa ccatttggtc actgttgaca gccgttgggt caggagaccg
     2821 atatcgaatt cgcgggaccg cgccagttcg tcttcactca gcaggtcggg catgagtgtg
     2881 ccgatgcctg ccttctgcaa ttgctccgcc accgcctggt tgagacggtt gagccggcta
     2941 ttcgcgccgg cgtgggagaa gataacgata caatcggcgt tgcccgggat caccaggtcg
     3001 gccggcacca tccgggttcc ggcggggata tgtatttcct gtttataggt gggcattttt
     3061 tttcttcaaa gatatcggcc gtcagcgggg aacctgctga gggtggtcag cgggccggat
     3121 gacaatcgtc agggttgaag tttatcatcc ggcgggttga cgccggtcat gcccgtggcg
     3181 ggatgaaggt tttactttcg gtacataaaa tgtaaagcca tgataggaaa attaactcca
     3241 ccggagatcg agaagctgtt gagcaaggag gtcgtggggc gcctggggtg cagcgatgga
     3301 gagatggtct atgtagtgcc gatcagcttt cactatgacg gtgaatacgt gtactgccat
     3361 acgcatgagg ggttgaaaat cgacatcatg cgaaaacatc cagttgtttg cttcgaggtg
     3421 gaccggatgc acaatatggc caactggcag agtgtcgttg cgcatgggcg cttcgaggaa
     3481 ttgaccgatc ccatcctgag gagccaggcc ctgcagcggc tgcatgaacg ggtgctcccg
     3541 atggtaagca gcgagacgac gcacctgtcg ccggattggc cttttacacc actggaattg
     3601 aataaaatag tgggcgtgac cttccgcatc cgcctggagg aaaagacggg ccggtatgag
     3661 aaatcccatt ctgtggctca gaccttcttt agtatctgaa ccataaaatc tacgaccatg
     3721 aacagaatag ggatccgggc agtagccgca ttgttgtggg cgggtgtctt tttgcctgcc
     3781 gccgggcaac aaccggagcg gttgaccatc tgggaagcgt accgggtggc gagggagaac
     3841 tatccttcct cgaagcagcg ggggctgatc gagaaaacga gagaatacac cctgcagaat
     3901 gcggcccgcg gttacctgcc gcaattgagt tttgcggggc aggcgaccat gcagtcggat
     3961 gtcacgaatc tgcccatcaa gatcccggtg gccggttttt ccatgccgca gtacagcaag
     4021 gatcagtata agatctatgg cgaggtggac caggcagtat atgatggcgg gatgatccac
     4081 aaccagcagg aggctgcgcg ggtggccgcc gccatccagg aaaagagtct cgatgtggag
     4141 ttgcataccc ttttcgaccg ggtggaccaa ttgtatttcg gggttttgct gctggatgcg
     4201 caactaagac agaacgacct gctgcgccag gatatccgca atggattgga gaagatgcag
     4261 gcgatggtgg acaatgggac ggcgtacaag agcggggtgg atgagttgca ggcgcagttg
     4321 ttacaggcgg gtgagacaca aacggaggaa aatgctaccc gcaagggtta tctgaatatg
     4381 ttattgatgc tgctcggaaa aacgcccggc gcgatggtgt cgctgacgga gccgccggag
     4441 ctatcgttgc ataccaccat cagccggcct gagctgcttt tgttcgagtt tcagcaacgg
     4501 aactatgatc tgcaggatga ggcggcggcg gcgcttatcc ggccgcggtt cagtcttttc
     4561 gtccagggcg gttattcgcg gcccgggctg aatatgctga gcaataattt ttccttttat
     4621 gcgctcggcg ggatccggat gagctggaat cttggcggtt ggtatacgtt gaagcggcag
     4681 cgcgccttat cggagatcgg gcggcgcggg ttagacctgc aaagggagac ctttttgctg
     4741 aacacgggta tgcagcagga gcaatccatc ctcgaggtgg ccaggtatac tgctttgttg
     4801 caggaggatg ggccgatcat cgaattgcga acggcggtga aagaagcggc gctggcgcaa
     4861 ttgaggcagg gtacgctcag tgcgcatgac tacctgacgc aggtgaatgc cgaggaccag
     4921 tcgcgtgaga ccagggtatt gcatcgcatt caactgctgc gaagtgaata tgcttatcaa
     4981 acccttatcg ggcattaaat cagctttttt atggtgaacg tttcagcgat agaaaagacc
     5041 tacgtgaccc gcaaggcgaa gacgaaggcc ttggacggca tttccttttc ggtggacaag
     5101 ggcgaagtgt ttgggatcat cgggccggat ggcgccggta agacctcgct tttccgtatc
     5161 cttaccaccc tgctcctgcc cgacgccggc accgcgatgg tgaacggcct ggacacggta
     5221 aaagattata aggagatcag gaagtgtgtg ggctatatgc cgggcaggtt ctcgctgtac
     5281 caggacctgt cggtggagga gaacctggag ttctttgccg ccatcttcga tgtggatatc
     5341 caacagaatt atgcgttgat cggggatatc taccggcaat tggagccatt caagggccgg
     5401 aaggcggggc aattatcggg tgggatgaag caaaagctgg ccttgtgctg tgcgctggtg
     5461 catcaaccgc tggtgttgtt
//
LOCUS       DATGFG010000934         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_50567, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000934 DATGFG010000000
VERSION     DATGFG010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50567"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1138
                     /locus_tag="VKQ52_17655"
     CDS             230..1138
                     /locus_tag="VKQ52_17655"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLZ89088.1"
                     /translation="MRYLFAGFIGLLGFCKGVVAQAPAFDSGLVVAEGAIRPQLVSRQ
                     FSFTEGASVDRAGNIFFTDQPNNKIWEYTLGGELVLFMDSAGRSNGMYFDRGGRLVTC
                     ADEEEQLWSIGPDKRVKILLRDIDGHRLNGPNDLWIDAKGGIYFTDPYYQRPYWARTH
                     PDLDGERVYYLPRGRKQPVVVAADLKKPNGIVGTPDGRMLYISDLGDGKTYVYSIGAD
                     GNLSGRRLLCLQGSDGMTLDERGNVYLTGDGVTVYDPGGRKISHIPVPEKWTANLCFG
                     GKDRKTLFITASEGVYLLRMRVRGVE"
     gene            complement(1171..1566)
                     /locus_tag="VKQ52_17660"
     CDS             complement(1171..1566)
                     /locus_tag="VKQ52_17660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ89089.1"
                     /translation="MLKYKKILLVDDDADDRQIFEEILRDIDPEAVVVTAENGLEMVS
                     LLDKMPDDDMPDMIILDQNMPKMTGKESLIFLKESPRYTHISTIVYSTYQVKDFYQEC
                     LELGAQDVVAKPDTMQAYREMIEQFLVAH"
     gene            complement(1717..>2597)
                     /locus_tag="VKQ52_17665"
     CDS             complement(1717..>2597)
                     /locus_tag="VKQ52_17665"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HLZ89090.1"
                     /translation="GGNFLSATPDTDYTAEALRRCRLTVHVSTKLNRSHLVHGEEALI
                     LPCLARSDRDYTSDREQFVSCENSMGVVQSSKGVLTPISTQLLSEPAIVCRLAMATLG
                     ERTKVDWKACLKDYDAIRDAIERTIPGFEEYNKRVREPGGFYLPNAPREGKFNTPDKK
                     ACFNIASPTTIPLEAGELMMMTIRSHDQFNTTIYGLNDRYRGIFNERRVILLNEADMR
                     AQNLAPNDIVDIYNDHGGVRRIAHRFVVVPYPIPRDCAATYFPETNVLIPIDSVAKKS
                     NTPVAKLVVIRLQRSS"
BASE COUNT          511 a          556 c          874 g          656 t
ORIGIN      
        1 gatccgggtt gtggggcgcc tggctgctgg gcgaggaccc aggtggcgaa gccggggatg
       61 tctccctgcg ggtttgtgag gatatatttc tcccactctg ataataaggg gattacagaa
      121 gtagcgatgc cgctcatttg caacaatttt ggtccaaatt acaaaatgaa taaacaaaag
      181 tgttttattg gcgaatgata ggatgaatta tcttgctcaa aaatcgtcta tgcggtattt
      241 gtttgccggg tttatcggtt tgctggggtt ttgtaagggg gtggtggcgc aggcgcctgc
      301 ctttgattcg gggcttgtcg ttgccgaggg cgccatccgg ccgcagctgg tgtcgcgtca
      361 attttccttt acggaggggg cgtcggtgga ccgggccggg aatatctttt ttacggatca
      421 gcccaataac aagatctggg aatatacgct tgggggggag ctggtgcttt ttatggatag
      481 cgccggcagg tcgaacggga tgtattttga ccgcgggggc cggcttgtca cctgtgccga
      541 cgaggaggag caattatggt cgattggtcc ggacaagcgg gtgaagatac tgttgcggga
      601 tatcgacggg caccggctga acgggcccaa cgatctatgg atcgatgcga agggggggat
      661 ctattttacg gacccctatt atcagcgacc ctactgggcg cggacgcacc cggaccttga
      721 cggcgagagg gtgtattatc tgccgcgggg gaggaagcaa cccgtggttg tggcggcgga
      781 tctgaagaag cctaacggga tcgtgggtac gccggatggc aggatgctgt atatatcgga
      841 tctgggggat gggaagacct atgtatattc tatcggtgcg gatgggaatt tatcggggag
      901 gcggttgtta tgcctgcagg ggtccgatgg gatgacgttg gatgagcggg ggaatgtgta
      961 tctgacgggg gatggggtga cggtgtatga cccgggggga aggaagatca gccatattcc
     1021 ggtgccggaa aaatggacgg ccaatctgtg ttttggcgga aaggacagaa agaccctgtt
     1081 tattaccgca tcggaaggag tgtatctcct gcggatgcgg gtcaggggtg tggagtagtt
     1141 accgggtttt atgccggcgt gtaccgggct ttaatgggct acgaggaatt gttcgatcat
     1201 ttcgcgatag gcctgcatgg tatcgggttt cgcgacgacg tcctgggcgc cgagctcgag
     1261 gcattcctgg tagaagtcct tcacctggta ggtagaatag acgatggtag agatatgggt
     1321 gtagcgtggg ctctccttta agaagatcag gctttcctta ccggtcatct ttggcatatt
     1381 ctggtcgagg atgatcatat ccggcatatc gtcgtcgggc attttgtcta acagggacac
     1441 catttccagg ccattctctg ctgtgacgac cacggcttcg gggtctatgt cgcgcaggat
     1501 ctcttcgaag atctggcggt catcggcgtc atcgtctacc aggagtattt ttttgtactt
     1561 cagcatactt ttcacaatag gtactttacc attttatgtg tggaaggaat ggggaattaa
     1621 ttccccattc ggctccattt atcaggtaac acgacgcttt tccttcaatg gagtagcaaa
     1681 ttatccatgg gaagtgaaca aaaacgcggc cattttttat gaggatcgtt gcaggcggat
     1741 gacgaccagt tttgcgacgg gggtattgct tttttttgcg acgctgtcga taggtatgag
     1801 cacgtttgtc tccgggaagt aggtagcggc gcaatcccgg gggatggggt agggaacgac
     1861 gacgaagcga tgggcgatgc ggcggacccc gccgtgatcg ttgtagatat cgacgatgtc
     1921 gtttggtgca aggttttgcg cgcgcatatc cgcttcattg aggaggatga cacgtcgttc
     1981 gttgaatatg ccgcggtagc gatcgttcag cccgtagatg gtggtgttga actggtcgtg
     2041 gctgcggatg gtcatcatca tcagctctcc cgcttctagg gggatcgtcg taggggacgc
     2101 gatattgaag cacgcttttt tgtcgggggt gttgaatttg ccttcccggg gtgcgtttgg
     2161 caggtagaaa ccaccgggct cgcgtacgcg cttgttatac tcttcgaaac cggggatggt
     2221 gcgctcgatg gcgtcacgga tggcatcata gtctttcagg caggctttcc agtcgacctt
     2281 tgttctttca ccgagggtcg ccatggccag ccggcaaacg atggcgggct cactgagcag
     2341 ttgggtggag atgggtgtca gtaccccctt tgaagactgg acgacaccca tcgagttctc
     2401 gcaggagacg aactgctccc tgtcgctggt gtagtcgcgg tcgctgcggg cgaggcaggg
     2461 gaggatgagg gcttcttcac catggacgag atggcttcgg ttgagttttg tcgagacatg
     2521 tacggtgagg cggcagcggc ggagggcctc cgcggtgtaa tcggtatcgg gggtggcgga
     2581 gaggaagttg ccgccca
//
LOCUS       DATGFG010000935         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1190926, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000935 DATGFG010000000
VERSION     DATGFG010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190926"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VKQ52_17670"
     CDS             complement(<1..478)
                     /locus_tag="VKQ52_17670"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HLZ89091.1"
                     /translation="MADPFSSYSIFPLGDSAITIDVGNCIDEQHNIKALAVHDWLQAH
                     RFPGVLDIIMAYSSVSVFYDPAVVAASGVNGPIGVFNWLEGLLERAWKEAGAAAGCGE
                     GGDEESPAGAGGGADGTGDDARLIRIPVCYEGEYAPDLGWVAAQKGLSPEAVVDLHA"
     gene            702..>2348
                     /gene="topA"
                     /locus_tag="VKQ52_17675"
     CDS             702..>2348
                     /gene="topA"
                     /locus_tag="VKQ52_17675"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HLZ89092.1"
                     /translation="MAKNLLIVESPAKAKTIEKILGEDFEVKSCYGHIRDLEKDDMGI
                     DIKNNYRPRYIVPVDKEKVVKELKSIAKKSGEVWLATDEDREGEAISWHLCEVLGLDP
                     GTTKRIVFHEITNTAIRSAVTKPRTVDMNLVNAQQARRILDRIVGFELSPVLWRKMSM
                     KNNLSAGRVQSVAVRIIAEREREINAFTAVSSFKIEALFTAKDVNGKAVTFSAEGKKQ
                     SSAENAEKFLQSCFNADYTVTDIQVKPGKKTPAAPFTTSTLQQEASRKLGYSVSRTMI
                     LAQKLYESGKITYMRTDSVNLSDLAMDATANQIKSQYGNKYHQARKFKNKNESAQEAH
                     EAIRPTYMDAMTVEDADCRRLYELIWKRTMASQMADAELEKTIAKIQISTNKEELTAS
                     GEVLKFDGFLKVYNEGRDEDDTAEGEEEGQEGMLPPLTVKQVLPLKEMRAVERFTRAL
                     PRYTEASLVKKLEELGIGRPSTYAPTISTILKRGYVEKRDKEGVKRDFRILLLKNDHI
                     EKLVEQENTGAEKSKLFPTDLGLVVTDFLKQYFDDIMDYHF"
BASE COUNT          651 a          716 c          559 g          422 t
ORIGIN      
        1 acgcatgcag atcgaccacc gcctccggcg acaacccctt ctgagccgcc acccacccga
       61 gatccggcgc atactccccc tcataacata ccgggatacg gatcaaacgc gcgtcatccc
      121 ctgtcccatc cgcccctccg ccagccccgg ccggcgactc ttcatcgcca ccctccccac
      181 atcccgcggc ggccccggcc tccttccagg ctcgttccag caacccctcc agccaattga
      241 acaccccaat ggggccattt acgccgctgg ccgctacaac cgccggatca taaaataccg
      301 acaccgaact atacgccata attatgtcca gcaccccggg aaatcggtgg gcctgcaacc
      361 agtcatgaac cgccagcgcc tttatattat gctgttcgtc aatacaattg cctacatcga
      421 tggtaatagc cgaatcgccc agggggaaaa tggaataaga agaaaatggg tcagccacgg
      481 cgtcagattt agtcaacaaa atacgccatt ttgaacaacc ggggctgccg atgtgttcat
      541 aaacttataa cttccatttc ttataggttt gtaaatgatt gattatcaat tatactgcaa
      601 ctgcctcaga cccctcccac ctgcccggcg cggccggctg attttttaat ttgatggtta
      661 tactgtaaaa ttgcaagcca acaggcatta ttaattacta catggcaaag aacttattaa
      721 ttgtggagtc gccggcgaaa gccaagacca tagagaagat actgggtgag gattttgaag
      781 taaagagctg ttatggccac atccgcgatc tggagaagga tgacatgggt atcgatatca
      841 agaacaacta ccgcccccga tatatcgtcc ccgttgataa agaaaaggtt gtcaaagagt
      901 taaaatccat cgccaagaaa tcgggcgagg tatggctcgc gactgatgag gaccgcgaag
      961 gggaagccat ttcctggcat ctgtgcgaag tcctcggcct cgaccccgga acgaccaagc
     1021 gcatcgtctt tcacgagatc accaacaccg ccatccgtag tgccgtcaca aagccccgga
     1081 cggtggatat gaatctggtc aatgcccagc aggcccgccg gatattggac cggatcgttg
     1141 gcttcgagct cagtcccgta ctctggcgca agatgagcat gaagaacaac ctcagcgccg
     1201 ggcgggtgca aagcgtcgcc gtgaggatca tcgccgaacg cgaaagagag atcaatgcct
     1261 tcaccgccgt gagctcattt aagatcgagg ccctcttcac ggcaaaggac gtcaacggca
     1321 aagccgttac cttctccgct gaaggaaaaa aacagtcctc cgcggagaat gccgagaagt
     1381 ttctccaaag ctgcttcaat gccgattata cggtaacgga catccaggta aaacccggca
     1441 aaaagacccc cgcggcccca ttcacgacct ccacgctcca gcaggaggcc agccgtaaac
     1501 tgggctattc cgtcagccgg accatgatcc tggcccagaa actgtacgaa agcggtaaga
     1561 tcacctacat gcgtaccgac agtgtcaacc tcagcgacct cgccatggat gccaccgcca
     1621 accagatcaa aagccagtac ggcaataaat accaccaggc ccggaaattc aagaacaaga
     1681 acgaatccgc ccaggaggcc cacgaagcca tccggccaac ctatatggat gccatgaccg
     1741 tcgaggatgc cgactgccgc cgcctctacg aactcatctg gaaaaggaca atggccagcc
     1801 agatggcgga cgccgaactg gaaaagacga tcgccaaaat tcagatctcc accaataaag
     1861 aagaactcac cgccagcggc gaagtcctga aattcgacgg cttcctgaaa gtctataacg
     1921 aaggccgtga tgaggacgat accgccgaag gagaagagga gggccaggaa ggcatgctcc
     1981 cgcccctaac ggtaaagcaa gtgctcccgc tgaaggaaat gcgggcagtc gaacgcttta
     2041 cccgcgcact gccccggtac accgaggcct ccctcgtaaa aaaactggaa gagctcggca
     2101 tcggccgccc ctccacctac gcccccacca tctccaccat cctcaaaaga ggatatgtcg
     2161 aaaaacggga taaggaaggc gtaaaaagag atttccgcat cctcctgcta aagaacgacc
     2221 atatcgagaa actggtcgaa caggagaaca ccggcgccga aaagtccaaa ttattcccca
     2281 ccgacctcgg tctggtggtg acggacttcc tcaaacaata cttcgacgac atcatggact
     2341 atcatttc
//
LOCUS       DATGFG010000936         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1557056, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000936 DATGFG010000000
VERSION     DATGFG010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557056"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VKQ52_17680"
     CDS             <1..799
                     /locus_tag="VKQ52_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89093.1"
                     /translation="PWSLLLYAAIVRKCRSRRTVPGDLICLGAALASFIVFSLSRFQL
                     PHYLNILFPFFSILTAAYLYGGVRRRWAQKTIRVMQDTIAVLLPILLLALSWFYRFDH
                     LPSLMGTILLVAILPFILFRGASLSIPFARSFWMALLVFSFVNFVLYPAILQYQAGTV
                     AGRYTATRPEPVSRPVYLLREAPVDYSFEFDCPHPVERIPIDSLADDALVFAPAAFAD
                     TLARHGYQASFVRTFPNFHVSQLTGKFINRDTRAGVLTPWSLMRVRR"
     gene            complement(853..1452)
                     /locus_tag="VKQ52_17685"
     CDS             complement(853..1452)
                     /locus_tag="VKQ52_17685"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ89094.1"
                     /translation="MSEKAEIREFQAGELLMKTGQNIRSTMLITKGLVKIFRVDEEGN
                     EFFMYYLQPGQACALSMICATKRETSEITARAVTQTESIIIPLEYMDKWMTQYKSWYH
                     FVLESYRSRFEELLLTVDHIAFRNMDQRLLFFLQKQMETLRTNKFTISITEIASDLNS
                     SREVISRLLKKLSDRGLIHLDRNLLEIVDLDMQDGARKK"
     gene            1637..2983
                     /locus_tag="VKQ52_17690"
     CDS             1637..2983
                     /locus_tag="VKQ52_17690"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ89095.1"
                     /translation="MLLNAQIGMAGRRGIVFLLLLVFSGASAQAPPVRLTAGEAITAA
                     LAGNQTAKIAAWDEKIAAARYKEMEAVYLPQAGVAYTAMTTNNPLNAFGFKLQQQSIV
                     SADFNPDQLNHPGGTPDFMARLDVQQPLLNIDQLYLRKGAAKEVEVYRLKAQRTREGL
                     VFEVRKAYMQLQLAEGSVSVLEEALATAGALFRYTSDRVEQGMLNKADALNARVQVTT
                     TESRLAEARSQVKNASDYLSLLMGRPLGMVYTADSSSAEVSETTPADKGVPAGRADLA
                     ALQKAVEAADLGIRSRKMSAFPRLNAFGSYQFNDSRMFGFGANAYLGGIQLSWDIFRG
                     NSIRNKVATQTFERNKLAEELSLRSAQSNLELNTARRQLADAGFRIQQQRTAVAGAAE
                     SFRILKDRYEQGLAGSTDVLLAQTQLSQQQLALVNALFDHRVAIAYIEFLTASATN"
     gene            2994..>3430
                     /locus_tag="VKQ52_17695"
     CDS             2994..>3430
                     /locus_tag="VKQ52_17695"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HLZ89096.1"
                     /translation="MKNILLTAVVAAPLLLAGCSGGRQPEAGETKDSPVTVTVTRPSS
                     AGAGEVMASGQVEAVQTAAISTRVMGTITRIFVKVGDRVNKGQLLATISSDDLAAKRA
                     QADAQIAGAQADLDNARKDYDRYTALFSRQSATASELDNATLRL"
BASE COUNT          718 a          959 c         1007 g          746 t
ORIGIN      
        1 gccctggagc ctgttgttgt atgccgccat cgtccggaaa tgcaggagcc gccggaccgt
       61 cccgggggac ctgatctgcc ttggcgcggc cctggccagc tttatcgttt tctccctgtc
      121 gcggtttcag ctgccgcact acctgaatat tcttttcccg ttcttctcca tcctcacggc
      181 cgcctatctc tatggcggcg tccgccgccg gtgggcacaa aagacgatcc gggtcatgca
      241 ggataccatt gcggtgctgc tgcccatcct cctcctggcc ttgtcctggt tctatcgttt
      301 tgatcatttg ccgtcgctga tggggacgat cctgcttgtt gccatcctgc cctttatcct
      361 tttcaggggc gcctcgttat ccatcccctt tgcgcgtagt ttctggatgg cgctgcttgt
      421 cttttccttt gtcaattttg tcctgtatcc cgccatcctg caataccagg cgggtacggt
      481 cgccggccgt tatacggcga cccggccgga accggtctcc cgtcctgttt acctgctgcg
      541 ggaagccccg gtggattatt cctttgaatt cgattgcccg catccggtgg agcggatccc
      601 cattgacagc cttgcagacg acgcccttgt ctttgccccg gcggcttttg ccgacaccct
      661 ggcccggcat ggttaccagg cgagtttcgt gcgaactttt cccaactttc atgtcagcca
      721 gctgacgggg aaattcatca accgcgacac gcgggccggt gtactgacac cctggtcgct
      781 gatgcgggta aggcggtgat gcgggtgagg cgcagatgcc ggtaagacgg tgatgcgggt
      841 aaggcgctga tcctatttct tcctggcgcc gtcctgcata tcaagatcga cgatctccag
      901 gaggttgcgg tcgaggtgga tcagccccct gtcggacaat tttttaagga ggcgggagat
      961 gacttcgcgg gaggaattga gatcggaggc tatttctgtt atggagatgg tgaatttgtt
     1021 ggtcctgagc gtttccatct gcttttgcag gaaaaaaagc agccgttggt ccatatttcg
     1081 aaaggctata tggtccactg tcagcaacaa ctcttcaaag cgggaccggt atgattccag
     1141 gacaaaatgg taccaactct tgtactgggt catccatttg tccatgtatt ccagcggaat
     1201 gatgatgctt tccgtttgcg tcaccgccct ggccgtgatc tcggacgtct cccgtttggt
     1261 agcgcagatc atcgacagtg cgcaagcctg tccgggctgc agataataca tgaagaattc
     1321 attgccctct tcgtctaccc ggaagatctt gacgaggccc tttgtaatga gcatcgtcga
     1381 ccggatgttc tgaccggtct tcatcaaaag ctcgcctgcc tggaattccc gtatttcggc
     1441 tttctcgctc atactaccga ccaaatccct ttcaaaggaa gggaacaggg tgcttatttg
     1501 tgcgtcttcg accatgggga tcactttagt cagcgacaaa tgtaatgaca acccgtaata
     1561 aatcaatcag tattgtcgtg accaatgtca cggttagtga ccgtgcacaa aaatagcttt
     1621 gcatcaaatt ataaacatgc tattgaatgc gcagataggg atggcaggaa gaagagggat
     1681 tgttttcctg ctgttgctgg tgttctccgg tgcttcagcc caggccccgc cggtcaggct
     1741 gacggccggg gaggcgatca ccgccgcgct tgccggtaac cagacagcaa aaatagctgc
     1801 ctgggacgaa aagatcgccg cagcccggta taaagagatg gaggcggtat acctgcccca
     1861 ggccggggtc gcctacaccg ccatgacgac gaataatccc ctgaatgcat ttgggttcaa
     1921 attgcagcag caatccatcg tgtcggcgga tttcaacccg gaccaactga atcaccccgg
     1981 tggaacgccg gatttcatgg caaggttgga cgtacagcaa ccgctgctga atatcgatca
     2041 gctctacctg cgcaaaggag ccgcgaagga ggtggaggtt taccggttga aggctcagcg
     2101 cacaagggaa ggattggttt tcgaggtcag gaaagcctat atgcagctgc aattggctga
     2161 gggatccgtc agcgtgctgg aagaagccct ggcgacggcg ggagcgcttt tccgctacac
     2221 cagcgacagg gtagagcagg gaatgctcaa taaggcggat gccctgaatg ccagggttca
     2281 ggtgacgact accgagagcc ggctggcgga agcgaggagc caggtgaaga acgcgtcgga
     2341 ttacctgagc ctgctgatgg gaaggcccct tgggatggtc tatactgccg acagcagcag
     2401 tgcggaggtc agcgaaacaa cccccgccga caagggcgtg ccggccggcc gggctgatct
     2461 tgccgcgttg caaaaggcgg tggaagccgc cgacctgggc atccgttcgc gtaaaatgag
     2521 cgcctttccc cggctcaatg cattcggctc ttaccagttc aacgacagcc gaatgttcgg
     2581 ttttggcgct aatgcctacc tgggcggtat ccagctgtcc tgggacatct tcaggggaaa
     2641 cagcatccgg aataaggtgg cgacgcaaac cttcgaacgc aataagctgg cggaggaact
     2701 gtccctgcgt tcggcgcaaa gcaacctgga gctgaacacg gcccggcggc agttggctga
     2761 tgcgggattc aggatccaac agcagcggac agccgtcgca ggcgcagccg agtcttttcg
     2821 catcctaaag gaccgctacg aacagggatt ggcgggcagc acggatgtcc tgctcgcgca
     2881 gacacagcta tcacaacagc agctggcgct ggtgaacgcg ctattcgatc accgggtggc
     2941 cattgcctat atagaattct taaccgcttc ggcaacaaat taaaaatagt caaatgaaaa
     3001 atattcttct tacagctgtt gttgcggctc cccttttgct ggcaggctgc tccggcggcc
     3061 gccagccgga agccggagag acaaaggaca gccccgtgac ggtaaccgtc accaggccct
     3121 cgagcgccgg cgccggggag gtgatggcca gcgggcaggt ggaagccgta caaaccgccg
     3181 cgatcagtac ccgggtcatg gggacgatca cccgcatctt cgtaaaagtg ggcgacaggg
     3241 taaacaaagg gcagctgctg gctactatca gcagtgacga cctggcggcg aagcgggcgc
     3301 aggccgacgc gcagatcgcg ggtgcgcagg ccgacctgga caatgcccga aaggactatg
     3361 accggtatac agcgctcttc agccggcaaa gcgctacggc cagcgaactg gacaatgcca
     3421 cgctgcggct
//
LOCUS       DATGFG010000937         6449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_963828, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000937 DATGFG010000000
VERSION     DATGFG010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963828"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VKQ52_17700"
     CDS             <1..742
                     /locus_tag="VKQ52_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89097.1"
                     /translation="MLMGTYGITRKLNVIAGAPYVWTHATAGTLHGQHGIQDLSAWVK
                     WETLEQNVGKGVLSVYLLGGGSIPLTNYIADFLPLSIGLQSKTLSGRLTVDYQVKNFF
                     ATASGTYTGRSNITIDRNSYYTTSLHLTNQVDMPDMASWDVRAGYRSDWLIAEAVFSD
                     MITTGGFDIRKNDMPFPSNKMNSTMAGVDFKYTFRKLNALSVVGGGMYTLSGRNVGQS
                     TSWDLGVFYVLDFSPKKSKTHTSAAAKK"
     gene            787..2328
                     /locus_tag="VKQ52_17705"
     CDS             787..2328
                     /locus_tag="VKQ52_17705"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLZ89098.1"
                     /translation="MNRTIFLLSVGTLFLLAACNKSVSDRTAGLPPLAPTNEDLTAGS
                     WKPILLSRPDTFAVAAPVATSNALYTADLNEIKGYQAHLSSDQRGQIAYWSAGGVLRW
                     NEIMRDLMAKYNLPPYQNADGSYPIPSSANPFAYPFFPFSNPPYAARAYAYVSAAQYD
                     ALVACWHYKTLYKRPAPYLVDSTIKAMAGKTSLPSYPSEGAVLAGVTAEMMQLLFPDE
                     IANIQQKLQQQESALIASGAAARSDINAGEALGRQVADVFIARARGDNAGKAVGTPAV
                     WAGLVTTAQAANQTPWLSLEAPARPPMLPLFGKTKGFLCDSATVVALRPGPPPSTGSD
                     VMKAQTQAALNTIKNATRDQMRIVQFWADGVGTYTPPGHWNAIAAEDFVTQNYSEVRW
                     ARNMALLNMAEMDAAISCWDIKYYYFNPRPTQMDPTIKTLTGIPNFPSYVSGHSMFSW
                     AAATILGHILPSRASAYSAMAQEAANSRIYAGIHYSIDCTTGMTVGQNVGNYAIKRAQ
                     GDGAE"
     gene            2449..4032
                     /gene="treF"
                     /locus_tag="VKQ52_17710"
     CDS             2449..4032
                     /gene="treF"
                     /locus_tag="VKQ52_17710"
                     /inference="COORDINATES: protein motif:HMM:NF009774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HLZ89099.1"
                     /translation="MKRLAGFLLLVVTSFLSFGQPIVTTPPDKIYGKLFVDVQMNRVF
                     PDGKTFVDCVPRRKPAEILADYEAQKETAGFDLKKFVLDNFDLPANPADTYKTNSSED
                     VVTHIRNLWSVLKRTPDKVVEGSSLLPLPYPYIVPGGRFREVYYWDSYFTMLGLKESG
                     EIEMIDNMVKDFAYLMENYGHIPNGTRTYYLGRSQPPFFSVMVELLAEAKGDSVFQEF
                     LPAMEKEYAFWMDGAEKLKPGEAYRRVVRLKGGDILNRYWDDALVPRQESWREDVLTA
                     QRSGRDKLEMYQHLRAAAESGIDFSSRWFADGRNLVTIRTTDFIPPDLNALLYHLEWA
                     ISKARLMNKDSTAAEFRRKAIRRGELLDKYCWNKTANFYTDYDFKTGKQSTHITPGGL
                     YPFCFINEHPDYMSFLGKKVATVIRGKLLQPGGIVTTTFDTGEQWDSPNGWAPLEWMT
                     IWGLDRCGQKELAADIAARWVKLVEKVYKETGKLMEKYNVVDLNKDAGGGEYPGQDGF
                     GWTNGVLLKLIRKYDLPKE"
     gene            4078..4920
                     /locus_tag="VKQ52_17715"
     CDS             4078..4920
                     /locus_tag="VKQ52_17715"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLZ89100.1"
                     /translation="MKLFSALLFSMSLFVTACSTTNVSVDDSLQRYFDSAGVRGDFGL
                     FDNGQGHFTIYNLKRFRDSAYSPGATFDILQSLIAVQTGIVKDDKAVLPAADSATRDS
                     VTGTATEVKAPLTLARAFQTGTITNDVVFQDLAKRIGKDTLKKWIDSLHYGNKDMGGF
                     PDGAFWKDDRLKITSDEQLGLVKKLYFDQLPFFQRTQKIVRGMFQVEANSNYRLAYKT
                     SQLALAGNHALGWVVGWVEENKHPYFFVLSVESTDARMDVSAVGLQLVKKILGPMGFF
                     AGNK"
     gene            4941..>6449
                     /locus_tag="VKQ52_17720"
     CDS             4941..>6449
                     /locus_tag="VKQ52_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLZ89101.1"
                     /translation="MYRLEHSEYLIGLGALPLLLAALFFLLSWKKRTAERMGDPALIR
                     QLIRNFSPLRFMIKVSVALLAFFIIVVAAANPQKPGAMENVQRKGVDVMLVLDVSKSM
                     LARDIKPSRLDKAKQLLLLLTEKLDNDRVGLILFAGRAYLQMPLTTDHGAARMYIQNA
                     SPDAVPTQGTVIGDALRMANSSFNSKERKYKTIVLVSDGEDHDPDALKAAKDLAQNGV
                     MINTIGIGSPEGSPIVDPATGELKKDEQGNTVISKLNEAELQQLSDATNGEYIRLDNV
                     DDALITMTQRIDNAEKKSMSDSEFIDYVSYFQWLVGAALLLLVMEFLLSERRGERAGQ
                     RAEKAERRVETAGGAIRTAVVLLVILAASAPAAAQTGNAQIRSGNRYYKKKQLDQSLQ
                     QYQSAVQQAPDNPTANYNLGNAQFRKNNFDEAAHSYDATVKNSQDKTMQEKGYYNKGV
                     AMVKQKKLQESIDAWKQALKLDATDADARENLEKALIQLKQQQQQQKNQNNQK"
BASE COUNT         1451 a         1787 c         1858 g         1353 t
ORIGIN      
        1 gatgctgatg ggaacctatg gcatcacccg gaagctgaac gtgatcgccg gcgcgcccta
       61 tgtctggacc catgccacgg ccggcaccct gcatggacaa catggtatcc aggacctttc
      121 ggcctgggtg aaatgggaaa cgctggagca gaatgtgggg aagggcgttc tttccgtcta
      181 cctgcttggc ggcggctcga tcccgctgac gaactatatc gcggacttcc tgccactgtc
      241 catcgggctg cagagcaaga ccctgtcggg ccggctgacg gtcgactacc aggtgaagaa
      301 tttctttgcc acggcgtcgg gaacgtatac cggtcgcagt aatatcacca tcgaccgtaa
      361 ctcctattac accacctcgc tccacctgac caatcaggtg gatatgcccg atatggcatc
      421 ctgggatgtc cgggcgggct atcgcagcga ctggcttatc gccgaagcgg ttttctctga
      481 tatgatcacg accggcggat tcgatatccg gaagaacgat atgccgtttc ccagcaacaa
      541 aatgaattcg accatggccg gggtggattt caagtatacc ttccggaagc tgaatgcgct
      601 gtcggtcgtg ggcggcggga tgtataccct gtccggccgg aatgtcgggc aatccacctc
      661 ctgggatctc ggggtcttct atgtcctgga tttctcgccg aaaaaatcca aaacccatac
      721 ttccgccgct gcaaaaaaat gatcgcctgc cggacgcgat ccggccacac aacaaaacaa
      781 aaagtcatga accgcactat atttcttctt tccgttggta cccttttcct gctggccgcc
      841 tgtaacaaaa gcgtttccga cagaacggcc ggtctgccgc cgctggcgcc caccaacgaa
      901 gacctcactg ccggcagctg gaagcccatt cttctttccc ggcccgacac ttttgccgta
      961 gcggctcccg ttgcgacgag caatgcattg tatacggcag acctaaacga aataaagggg
     1021 taccaggccc atctttccag tgaccagcgg ggccagatcg cgtattggag cgcgggtggc
     1081 gtgctgcgct ggaacgagat catgcgcgac ctgatggcca aatacaatct cccaccctac
     1141 cagaacgccg acgggagtta tcccattccc agcagcgcca accctttcgc gtatcccttc
     1201 tttcctttct ccaacccgcc ttatgccgcc cgcgcttatg cgtatgtaag cgcggcccaa
     1261 tatgatgcgc tggtagcctg ctggcattat aagacattgt acaaacgccc cgccccttat
     1321 cttgtcgatt ccactatcaa ggcgatggcc ggaaagacga gcctgccctc ctatccttcc
     1381 gagggcgccg tgctggcggg ggtcaccgcg gagatgatgc aattgctctt ccccgacgag
     1441 atcgcgaata tccagcaaaa gctgcagcaa caggagtcgg cgctcatcgc ctccggtgcg
     1501 gcggcccgta gcgatatcaa tgccggcgag gccctgggcc gccaggtggc cgatgtgttc
     1561 attgcccgcg cccgtggcga caacgccggt aaagcggtgg gcacgccggc cgtctgggca
     1621 ggtctcgtca cgacggcgca ggctgccaac caaactccct ggctcagcct cgaggctccg
     1681 gcaagaccgc cgatgctgcc gttgtttggg aagacaaagg ggtttctgtg tgactccgcc
     1741 accgtcgtgg cgctgcgccc tggtccgccc ccttccacgg gcagcgacgt gatgaaggcg
     1801 caaacccagg ccgctttgaa tacgataaag aatgccaccc gcgatcagat gcggatagtc
     1861 cagttctggg cggatggggt cggaacctac accccgcccg ggcactggaa tgccatcgcc
     1921 gccgaagact ttgtgaccca gaattacagc gaggtgcgat gggcgcggaa tatggccctg
     1981 ctgaatatgg cggagatgga tgccgccatc agctgctggg atatcaaata ttattatttc
     2041 aacccccggc ccacccagat ggacccaacg atcaagaccc tgacggggat cccgaacttc
     2101 ccctcctatg tctcggggca ctcgatgttc agctgggctg ccgcaaccat tctcggccat
     2161 atccttcctt cccgggcatc ggcctattcg gccatggcgc aggaagcggc caactcccgg
     2221 atctatgcgg gcatccatta cagcatcgac tgtacgacgg ggatgacggt gggtcaaaac
     2281 gtgggtaatt atgcgataaa acgagcccag ggcgatgggg ccgaataact attttttatg
     2341 gtttataagc aaaagcaagc aaaacgccgc gattcttcgc ggtgttttta ttttgggcct
     2401 ccccggtttg ggccggattc gctattttta gtatctataa cagcatatat gaaaagactg
     2461 gccggcttct tactgcttgt agtaacctcc tttctttctt tcggacagcc aatcgtcacc
     2521 accccacccg acaagatcta tgggaaatta tttgtggacg tgcagatgaa ccgggtcttc
     2581 ccggacggca aaacctttgt ggactgtgtc cccaggcgga agcccgcgga gatcctggcg
     2641 gattatgagg cgcagaagga gacagccggc ttcgatctga aaaaattcgt gctggataat
     2701 ttcgatctgc ccgccaatcc cgccgatacg tataagacca acagttccga agacgtggtc
     2761 acgcatatcc gtaatctctg gtcggtgctg aaaaggacac ccgataaggt ggtggaaggc
     2821 agttccctgc ttccgttgcc ctatccctat atcgtcccgg gcggccgttt ccgggaggta
     2881 tattattggg attcctattt taccatgctc gggctgaaag agagcggaga gatagagatg
     2941 atcgataata tggtaaagga tttcgcctat ctgatggaga attacggcca tatcccgaac
     3001 gggacccgaa cctattatct cggccgcagt cagccgccct tcttctccgt catggtggaa
     3061 ttgctggcag aggcaaaagg cgatagcgtt ttccaggaat ttctgccggc gatggagaag
     3121 gagtatgcct tctggatgga tggcgccgag aagctaaagc ccggggaagc gtatcgccgc
     3181 gtcgtcaggt tgaaaggcgg ggatatcctc aatcgttact gggacgacgc cctcgttccc
     3241 cgccaggaaa gctggcgcga ggacgtgctg acggcgcagc gttcgggccg ggataaactg
     3301 gaaatgtacc agcatctgcg ggccgcggcg gagagtggta tcgatttcag cagccggtgg
     3361 tttgcggacg gtaggaacct ggtgacgatt cgcacgactg atttcatccc acccgatctc
     3421 aatgcgctgc tctatcatct tgaatgggcc atctccaagg cgaggctgat gaataaggac
     3481 agcacggccg cggaattccg ccgcaaggcg atccggcggg gcgaactcct cgataaatat
     3541 tgctggaaca agaccgcgaa tttttacacc gattatgatt tcaagaccgg caaacagagc
     3601 acgcatatca ctccgggcgg gctctaccct ttttgcttta taaacgagca ccccgactat
     3661 atgagctttc ttggcaagaa agtggctacc gtcatccgcg ggaagctgtt gcaaccgggt
     3721 ggcatcgtca ccaccacctt cgataccggt gaacaatggg actcgccaaa cggctgggcc
     3781 ccgctggagt ggatgaccat ctgggggctc gaccgctgtg gccagaagga gctggccgcg
     3841 gatattgccg cgcgctgggt caaactggtc gagaaagtat acaaggagac ggggaagctc
     3901 atggagaaat acaatgtcgt cgacctcaat aaggatgcgg gtggcgggga gtatcccggg
     3961 caggacggtt ttggctggac caatggcgtg ttgctgaagc tgatcaggaa gtatgatctc
     4021 cctaaagaat agcttcaccg ggatttttgg gtggagtata tttgcgcaaa ctattgcatg
     4081 aaactgtttt ccgcccttct tttctcaatg tcccttttcg ttaccgcctg ttccacgacg
     4141 aatgtgtctg tggacgatag cctccagcgc tattttgact cggccggggt gcggggtgat
     4201 tttggacttt tcgacaatgg ccaggggcat tttacgatct acaatctaaa acggtttcgg
     4261 gacagcgcct attcccccgg cgccaccttc gatatcctgc aatcgctgat cgctgttcag
     4321 acgggtatcg ttaaggatga caaggccgtc ctgccggcag ccgattcggc aacgcgggat
     4381 tcggtgaccg gcaccgccac agaggtaaag gctccgttga ccctggccag ggcctttcag
     4441 acggggacga tcaccaacga tgtcgtattc caggacctgg cgaagcgcat cggcaaggac
     4501 acactgaaga aatggatcga ttcccttcat tatggcaata aggatatggg tgggttcccc
     4561 gacggcgctt tttggaagga tgaccggctg aagatcactt ccgatgagca gctgggtctt
     4621 gttaaaaagc tctacttcga ccagcttccc tttttccagc gcacccaaaa gatcgtgcgg
     4681 gggatgttcc aggtggaggc gaactccaat taccgcctcg cttataagac cagccagctc
     4741 gcccttgcgg ggaatcatgc cctgggctgg gtggtgggtt gggtggagga gaacaaacac
     4801 ccctatttct ttgtcctcag tgtggagtcg accgatgccc ggatggacgt ctcagccgtt
     4861 gggcttcaac ttgttaaaaa aatcttagga cccatggggt tctttgctgg gaataaatag
     4921 tatggcgtat cttgcaaatc gtgtatcgtc tcgaacatag cgaatacctg atcggcctgg
     4981 gcgccctccc cctcctgttg gcggcgttgt tcttcctgtt gagctggaag aagagaacgg
     5041 ctgagcggat gggtgatccc gcgttgatcc ggcagctgat ccggaatttc tcgcccctgc
     5101 ggtttatgat aaaggtcagc gtggcccttc tcgccttttt tatcatcgtc gtggcggcag
     5161 ccaatccaca gaagcccggc gcgatggaga atgtccagcg gaaaggggtg gatgtgatgc
     5221 tggtgctgga tgtgagcaag agcatgctgg cccgggatat aaagccgagc cggctggaca
     5281 aggcgaagca gttgctgttg ctgctgaccg aaaagctcga caatgaccgg gtggggttga
     5341 tcctctttgc cggtcgcgct tatctccaga tgccgcttac gaccgatcat ggcgcggccc
     5401 ggatgtatat ccagaatgcc agcccggatg cggtacccac gcagggtacg gtcatcggtg
     5461 atgctttgcg gatggccaat tcatcgttca acagcaagga acggaaatac aaaaccatcg
     5521 tcctcgtctc ggatggcgaa gaccatgacc ccgatgcgtt gaaggcggcg aaggacctgg
     5581 cgcagaacgg cgttatgatc aataccatcg gcatcggctc gcccgaaggc tcgccgatag
     5641 tagatcctgc caccggcgaa ctgaaaaagg atgagcaggg caacaccgtt atctccaagc
     5701 tgaacgaagc cgagctacag caattgtccg atgccaccaa tggcgaatat atccggctcg
     5761 acaatgtcga cgatgcgctg atcacgatga cccagcggat cgacaacgcc gaaaaaaaat
     5821 cgatgagcga ctcggaattc atcgactatg taagctattt ccagtggctg gttggggcgg
     5881 ccctgctcct gctggtgatg gaatttcttc tttccgaacg gcgcggggaa cgggccgggc
     5941 aacgggcgga aaaggctgaa cgccgcgtgg agacggcagg tggcgccatc cgcaccgcgg
     6001 tggtgttgct ggtcattctt gcggcttcgg cgccggcagc ggcacagacg ggcaatgccc
     6061 agatccgcag cgggaaccgc tattataaaa agaagcagct cgatcagtcc ctgcagcaat
     6121 atcaatccgc cgttcagcag gcgcccgaca atcccacggc caattacaat ttgggtaatg
     6181 cccagttcag gaagaataat ttcgacgagg ccgcgcactc ctatgacgcc acggtgaaga
     6241 acagccagga caagaccatg caggaaaagg gctattataa caagggcgtg gcgatggtca
     6301 agcagaagaa attgcaggag agcatcgatg cgtggaagca ggcgctcaaa ctggatgcaa
     6361 cggatgccga tgcaagagag aacctggaga aggcgctgat acagctgaaa cagcaacagc
     6421 agcaacagaa gaaccagaac aaccagaag
//
LOCUS       DATGFG010000938         5603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_509096, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000938 DATGFG010000000
VERSION     DATGFG010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509096"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="VKQ52_17725"
     CDS             complement(<1..2000)
                     /locus_tag="VKQ52_17725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89102.1"
                     /translation="MKSWRTAGLFCLLPFCALSLIAQEVPVTIRGTVHTATGVPLAGV
                     SIMIKGTQRGTTTSAEGSFTLSAPRNSTLVLSYTGYATKEVKIGAGEQQLTLQLEESR
                     NDMDQVIVVGYGTRRKSEVTGSIASVSEKAIQDVPSANLSQAIQGQAAGIDIEKSKGN
                     SHPGQSPSILIRGTRSVKAGNDPLFVVDGIPFQGSIDDINQDDVASVEVLKDASSTAI
                     YGSRGANGVILVTTKRGRAGMKPRVTYSGYAGFTKWLGEYPMMNGPQYLQLKKWATYL
                     GNPGVYTGPEDPNLINGSLIALAPEERQGIASGRSTDWQKLVYRTGISTNHQVGVSGG
                     TDNTQYALGAGYYDEKGVYPGQEFIRYSLKLSVDQRLGDYIRIGVSSLNTFTITRGES
                     VNPMGQALRASPLVTPYDSTGKLINNFLPGSANQVWNPLADFLPGNSVENRNRLGTFS
                     TVYVEFSPIKDLKYRFNGGAEVRNDVYGNFYGRYSTNNLGVNSTAENATILSTSYTLE
                     NLLIYDKTIARLHKINFTGLYSFQKYSIQNNTFDYTSVLSDQAQYYNPALGANFQATN
                     STTDPNKNNLMPWALISYMGRLNYTFNDRYLLTVTMRSDGSSKLAPGSKYHIFPSAAV
                     AWNAIKEKFLMSQTALTNLRVRGSFGEVGNPAINPYQTLGSLS"
     gene            complement(2192..3607)
                     /locus_tag="VKQ52_17730"
     CDS             complement(2192..3607)
                     /locus_tag="VKQ52_17730"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLZ89103.1"
                     /translation="MKKLLIIGCFVMVAAVYGSAQPLVKEMTGAGNAVSGRDTLTRDE
                     RMGWWREARFGMFIHWGVYAELAGEYDGHTIGKAAEWIMNRGKIPVATYQQVARRFNP
                     VKYDADAWVRLAREAGMKYIVITAKHHDGFALFGSKASKWNVVDATPYGKDLLKPLVA
                     ACRKYGIKLGFYYSQAQDWNNPGGAAARKVTSEGWPNPDSAKIDAYTAAHSGHWDPVQ
                     TSDSMEDYIDRVAVPQVKELLTGYGDVAVLWWDTPTGMTDEYAEKLHALLALQPKIIT
                     NDRLKRPDFPGDYKTPEQKIPNLSDLDGKDWETCMTMNGTWGFKASDHNWKSTATLIH
                     NLVDIASKGGNYLLNVGPTAEGEIPQPSIDGLKAIGAWMKVNGEAIYGTTASPLQPLA
                     WGRCTKKVSGASTTLYLSVFEWPANGKLVVPGVKGEVKKASLLAGGSKLSTENGADGL
                     VISVPATAPDAIASVIKVELK"
     gene            complement(3701..>5603)
                     /locus_tag="VKQ52_17735"
     CDS             complement(3701..>5603)
                     /locus_tag="VKQ52_17735"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HLZ89104.1"
                     /translation="FTRYLQWLTTHPYGREEMNTLNNHATCWVMQVAAFARFTGNDTL
                     MDLCRQRFKTVLLPGQMGADGSFPLELKRTKPYGYSLFNLDAMATICQLLSTPGDDLW
                     TYTTEDGRNMEKGIGFLYPYIADKSKWPFAHDVMYWDQWPVAQPALVFGAEAFGRRDW
                     LETWVRLNHEPENGEVIRNLPVRHPLIWMRAASRQGRSGVGSPVDLPVVMAEVERQTE
                     VMLKAIDSAKDRGAEGGGAGSIRANGVPGGATGSSAAGGAGGAGEPGSLPFSPRTIEN
                     GKLKLVTSRDWTSGYFPGELWFLYNFTGSGEWKEQAKVFTAAMEREKTNGTTHDVGLK
                     INCSVGAGYRYTDDPKYKQVMIDAARVLSTRFNPKIGCIRSWDHHRELWQYPVIIDNM
                     MNLELLFEATQLTGDSSFYRIAVSHANTTMKNHYRKDYSSWHVVEYDTVTGEVRRKMT
                     WQGYSDSSAWSRGQAWGLYGYTMCYRYTHDPRYLQQAEHIAAFILHHPRLPVDKIPYW
                     DFDAPGIPRTGQPVGGMGALSATAAAKDTVPRDASAAAVLASGLYELSHYSRMGKRYR
                     STADTILVNLTKYYRSPVGANDGFILLHSTGTRRTNTEVDEPLCYADYYYLEALSRSS
                     YTAGPGVDR"
BASE COUNT         1200 a         1897 c         1274 g         1232 t
ORIGIN      
        1 gacagcgagc ccagggtctg gtaaggattg atggcgggat ttcctacctc cccaaagctt
       61 ccccgcacgc gaagattggt caacgccgtc tgactcatca aaaatttttc ctttatcgca
      121 ttccaggcaa cggcagcgga cggaaaaata tggtatttgc tccccggcgc caatttggag
      181 gaaccgtcag aacgcatggt caccgtcaac aggtacctgt cattgaaggt gtaattcagc
      241 cggcccatgt aagagatgag cgcccagggc atcaggttgt tcttgttggg atcggtggtg
      301 ctgttggtag cctgaaaatt ggcgcccagg gccgggttgt aatactgcgc ctggtccgaa
      361 agcacgctgg tatagtcaaa ggtattgttc tgtatgctgt atttctgaaa gctgtacaac
      421 ccggtgaaat tgatcttgtg cagcctggcg atggtcttat catagatcaa caggttctcc
      481 agcgtgtagc tcgtcgatag gatggtcgca ttctccgccg tggaattgac accgagatta
      541 ttggtggaat atcgcccgta gaaattccca tacacgtcgt tcctgacctc ggcgcccccg
      601 ttgaaccggt atttcaggtc tttgatcggg ctgaattcga catataccgt ggagaaagtg
      661 cccagccggt tccggttctc aaccgaattg cccggcagga aatccgccag cgggttccac
      721 acctggttcg cactccccgg caggaaattg ttgatcagtt tccccgtgct gtcatatggc
      781 gttaccagcg ggctggcccg taaggcctgc cccatcgggt tgacactctc accccgggtg
      841 atcgtaaacg tgttcaacga actaacgccg atcctgatat agtcacccaa ccgttggtcg
      901 acgctaagct tcagggaata tcgaataaac tcctggcccg gataaacccc cttctcgtca
      961 taatacccgg cgccaagcgc atactgggta ttatccgttc cgcctgacac cccaacctga
     1021 tggttggtgc tgatccccgt cctgtagacc agcttctgcc agtccgtgct gcgcccgctg
     1081 gcaataccct gccgctcctc gggcgccagc gcgatcagcg agccgttgat caggttggga
     1141 tcctcgggtc cggtatagac acccggattc ccgagatagg tggcccattt ctttaattgc
     1201 aggtactgcg gtccgttcat catcgggtat tccccaagcc atttggtaaa accggcatac
     1261 ccgctgtacg tgaccctcgg tttcatcccc gcccggcccc gtttggtggt gaccaggatc
     1321 accccgttgg cgcccctcga tccatagatg gcagtggagg aagcatcctt caacacttcc
     1381 accgacgcga catcgtcctg gttgatatcg tcaatactac cctggaaggg gataccgtcc
     1441 acgacaaaca gcgggtcatt gcctgcctta accgaccgcg tgccgcgaat aagaatggag
     1501 ggcgactgac cggggtggct attgcccttg ctcttctcga tatcgatccc tgccgcctgg
     1561 ccctgtatcg cctggctcag gttggcggaa ggcacatcct ggatggcctt ctcactcacc
     1621 gaagcgatgg aacccgtaac ctcggacttg cgccgggtcc cataccccac gacgatcacc
     1681 tggtccatat cattcctgga ctcctcgagc tgcagcgtca gctgctgctc acccgcacca
     1741 atttttactt ccttcgtcgc ataaccggta tacgacagga caagcgtgct gttacgcggc
     1801 gcggaaagcg taaaagaccc ctccgcactc gtcgtcgtcc cacgttgcgt acccttgatc
     1861 atgatcgata cccccgccag cggcacgccg gtggcggtat gaacagttcc ccgaatagtc
     1921 accggaacct cctgcgcgat aagcgaaagc gcacaaaacg gcaagagaca aaataggccg
     1981 gcagtcctcc aacttttcat atgagcagtt ttagttaata aaaaaaagca atttgtaact
     2041 actgcaacta cgctatattt tagcctcgct gctttcgctc aaattgcttt ttattcaact
     2101 atattgtcaa ccctgctctt ttaccggacc attccctgca tctctacttc aattcggcca
     2161 ctctccccta ctgcaattcg accacactcc cctacttcaa ttcgaccttt ataaccgaag
     2221 cgatcgcatc cggcgccgta gccggaacac tgatcaccaa cccatccgcc ccattctccg
     2281 tcgacagctt actgccgccc gcaaggaggc tcgccttctt tacctccccc ttcacacccg
     2341 gcacaacaag cttcccattg gccggccatt cgaatacgga taaataaagc gtcgtcgaag
     2401 ccccactcac tttcttcgta caccgtcccc aggccagcgg ctgcaacggg ctcgccgtcg
     2461 ttccgtaaat agcttcccca ttcaccttca tccaggcgcc gatcgccttc aacccatcga
     2521 tactcggctg cgggatctcc ccctccgccg taggccctac attcaacagg taattgccac
     2581 ccttggacgc aatatccacc aggttgtgga tcagcgtggc cgtcgacttc cagttatgat
     2641 cggaggcctt gaagccccag gttccattca tcgtcataca ggtctcccaa tccttcccat
     2701 cgaggtcgct gagattgggg atcttctgct cgggggtctt atagtcgccg gggaaatccg
     2761 ggcgcttcaa ccggtcgttg gtaatgatct ttggctgtag cgccagcaag gcatgcagtt
     2821 tctccgcata ttcatccgtc atcccggtcg gcgtatccca ccacaacacc gcaacatcgc
     2881 catacccggt gagcagttcc ttcacctgag gaacggccac cctgtcgata taatcctcca
     2941 tcgaatccga ggtctgaaca gggtcccagt gaccactgtg cgcagccgta taggcatcga
     3001 tcttcgcgga atcgggattc ggccagccct ccgacgttac cttccgcgca gccgcaccgc
     3061 ctggattgtt ccagtcctgc gcctgcgaat aataaaatcc cagtttgatc ccatacttgc
     3121 gacaggccgc gaccaggggc ttgagcaggt ccttgccata cggcgtggca tccacgacat
     3181 tccatttgct ggccttgctg ccaaacagcg caaacccatc gtgatgcttg gcggtgataa
     3241 cgatatactt catccccgcc tccctggcca gccggaccca ggcatcggca tcgtatttca
     3301 ccgggttgaa ccgccgcgcc acctgctgat aggtcgcaac agggatcttc ccccggttca
     3361 tgatccactc cgccgccttc ccgatcgtat gcccgtcata ctctcccgca agctccgcat
     3421 acacccccca atggataaac atcccgaacc gcgcctcccg ccaccagccc atccgctcat
     3481 cccgggtcag ggtatcccgg ccggataccg cattcccggc gcccgtcatc tccttcacca
     3541 gcggctgtgc agatccgtac accgccgcca ccataacaaa acatccaata atcaaaagct
     3601 tcttcatatg aaaaacaatt tattgcaact aacaaaacat tccctcactc ccgttacccg
     3661 gaaatcacca atcattcaac tatcttggca tatcgccccc tcaccggtcc actcccggcc
     3721 cagccgtata cgaactccgg gacaatgcct ccaggtaata ataatccgca taacacagcg
     3781 gttcatccac ctcggtattc gtgcgcctcg tccccgtact atgcaaaagg ataaacccat
     3841 cgttggcgcc caccgggctc cggtaatact tcgtcagatt caccagtatc gtatccgccg
     3901 tcgatcggta cctctttccc atccggctat aatggctcag ttcatataac cccgaggcca
     3961 acaccgccgc cgccgacgca tcccgcggaa cagtatcctt tgccgcggca gttgcggaca
     4021 acgcccccat cccgccaacc ggctggcccg tccgcggaat acccggcgca tcaaaatccc
     4081 aataaggtat tttatccacc ggtagccttg gatgatgcaa aataaaggcc gcgatatgct
     4141 cggcctgctg caaataccgg ggatcatgcg tatagcggta acacatcgta tagccataca
     4201 aaccccaggc ctgcccacgc gaccaggccg acgaatcgct atacccctgc caggtcatct
     4261 tccgccgcac ctcccccgtc accgtatcat attccacgac atgccagctg ctgtagtcct
     4321 tccggtaatg gttcttcatc gtcgtattgg cgtgactcac cgcgatccgg tagaacgaac
     4381 tgtcccccgt aagctgcgtc gcctcgaaca acaactccag gttcatcata ttgtcgatga
     4441 tcacaggata ctgccaaagc tcccggtgat gatcccagct ccggatacac ccgatcttcg
     4501 ggttgaaccg cgtcgacaag acccgcgccg catcgatcat cacctgttta tattttggat
     4561 catccgtata ccgataccct gcgccaacgc tacagttgat cttcagccct acatcatgcg
     4621 tcgtgccgtt ggtcttctcc cgttccatcg ccgcggtaaa caccttcgcc tgctccttcc
     4681 attccccgct ccccgtaaaa ttataaagga accaaagctc ccccgggaaa tacccgctcg
     4741 tccagtcccg cgaagtcacg agcttcaatt tgccgttctc gatcgtcctc ggcgaaaaag
     4801 gcagactccc cggctcaccc gccccacccg caccgccagc cgccgaactc ccggtcgccc
     4861 cacccggcac cccattcgcc cggatactcc ccgcaccgcc accctccgca ccccggtctt
     4921 tcgccgaatc gatcgctttc agcatcacct ccgtctgcct ctccacttcc gccatcacga
     4981 ccggcagatc caccggcgat ccaacgccac tccttccctg ccggctggcc gccctcatcc
     5041 agatcaacgg atgtcttacc ggtaaattcc ggatcacctc cccattctcc ggctcatggt
     5101 tcagccgcac ccaggtctcc agccaatccc tccgtccaaa cgcttccgct ccaaaaacga
     5161 gcgccggctg cgccaccggc cattgatccc agtacatgac atcatgtgca aacggccatt
     5221 tgcttttatc ggcaatatag ggatacaaaa aaccgatccc cttttccata tttcgcccat
     5281 cctccgtcgt atacgtccag agatcatccc ccggagtgga caacagctga cagatcgtag
     5341 ccatcgcgtc gaggttgaaa agcgaatacc catagggctt cgtccgcttc agctccagtg
     5401 ggaaactccc gtccgccccc atctgcccgg gcaacaacac cgtcttgaaa cgttgccggc
     5461 agagatccat cagcgtgtca ttcccggtaa accgcgcaaa agcggccacc tgcatcaccc
     5521 agcaggtagc atggttattc agcgtattca tctcctcccg gccataagga tgcgtggtca
     5581 gccattgcag gtatcgcgta aac
//
LOCUS       DATGFG010000939         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_164429, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000939 DATGFG010000000
VERSION     DATGFG010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164429"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..687
                     /locus_tag="VKQ52_17740"
     CDS             211..687
                     /locus_tag="VKQ52_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89105.1"
                     /translation="MRKTLFVLSIVSFLFTTRGSAQAEGGSYQTAVGVKFWPGALTIK
                     HFISDDRALEGLLSFWDNGFRLTGLYEIHGDINGAPGLKWYVGPGAHIGWYNGAVYHN
                     YYYNSGALSIGVDGILGLEYKFEGAPIAISADINPYIEFNHPYFDIFGGLGVKYTW"
     gene            complement(706..1674)
                     /gene="ftsY"
                     /locus_tag="VKQ52_17745"
     CDS             complement(706..1674)
                     /gene="ftsY"
                     /locus_tag="VKQ52_17745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HLZ89106.1"
                     /translation="MGLFDKLFGKKEKESLNQGLQKTKEGFLTRLTKAVAGKSTVDEE
                     VLDNLEDALVSADVGIDTTVQIIKQIEKRVARDKYINTSELNGLLQEEIENILVEPKE
                     QSYRDFSTPSGSKPYVILVVGVNGVGKTTTIGKLAYNFKKAGKSVLLGAADTFRAAAV
                     DQLTIWSERVGVPIIKQGMGADPGAVAFDTVQSGVARGADVVIIDTAGRLHNKAYLME
                     ELSKISRVVKKIMPDAPHEVLLVLDGSTGQNALEQARQFTAATEVTSLAITKLDGTAK
                     GGVVLAIANQFRIPVKFIGVGEKMEDLLVFDKHEFVDSLFNLEGPR"
     gene            complement(1769..1942)
                     /locus_tag="VKQ52_17750"
     CDS             complement(1769..1942)
                     /locus_tag="VKQ52_17750"
                     /inference="COORDINATES: protein motif:HMM:NF025493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HLZ89107.1"
                     /translation="MAKAAKTAIKTKDAKAAAEAKNWTKVIKAVRSPKTGAYTFKEAI
                     VHKEKIKEYLAQK"
     gene            complement(1945..2127)
                     /gene="rpmG"
                     /locus_tag="VKQ52_17755"
     CDS             complement(1945..2127)
                     /gene="rpmG"
                     /locus_tag="VKQ52_17755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HLZ89108.1"
                     /translation="MAKKGSRVQVILECTEHKTSGKPGTSRYITTKNKKNTPERLELK
                     KFNPILKKVTVHKEIK"
     gene            complement(2152..2388)
                     /gene="rpmB"
                     /locus_tag="VKQ52_17760"
     CDS             complement(2152..2388)
                     /gene="rpmB"
                     /locus_tag="VKQ52_17760"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HLZ89109.1"
                     /translation="MARVCQITGKVPVSGNSVSHSNIKTKRRFLPNLQTKRFFLAEED
                     KWITLKVSSEGLRTINKNGLYSVVKKLRANGEKI"
     gene            complement(2527..>2728)
                     /locus_tag="VKQ52_17765"
     CDS             complement(2527..>2728)
                     /locus_tag="VKQ52_17765"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89110.1"
                     /translation="GSLDGHFRAFDRRTGAMLWEYALPAGGQATPMTYSLDGKQYVVI
                     AAGGHGKLKTKQGDYVIAFEVK"
BASE COUNT          586 a          822 c          584 g          736 t
ORIGIN      
        1 tgcccgctca ggacttcgct gtccaccata ttcttgctgc acccaagggt gatgatattg
       61 accttatccc gttgtatact tcttgtcttc ataaaaaaat tctcccggcg aacctccggt
      121 ccgtttgggg ttgcaaattt acaaacaatt gatcatctta ataatggcat ggttttttat
      181 atatttgcct gcacaaaaag tacatcgact atgagaaaaa ccctatttgt cctcagcatc
      241 gtctccttcc tctttaccac ccgtggatct gcccaggcgg aaggcggcag ctatcagacc
      301 gccgtcgggg tcaaattctg gccaggagca ttgactatca agcatttcat atctgacgac
      361 cgcgccctgg aagggctcct gagcttctgg gacaatggtt tccggctcac cggtctctat
      421 gagatccacg gcgatatcaa cggagcaccc ggtctgaaat ggtatgtcgg ccccggcgcg
      481 cacatcggct ggtacaacgg agccgtttac cacaactact actacaacag cggtgcactc
      541 tccattggtg tggacgggat actgggcctc gagtataaat tcgaaggcgc ccccatcgcg
      601 atcagcgcgg atatcaatcc ctacatcgag ttcaaccacc cctatttcga catctttggc
      661 ggactgggcg taaagtacac ctggtaaatt atcacagaag gcccgctagc gcgggccttc
      721 taaattaaat aagctatcga caaattcatg cttgtcaaaa acaagcagat cctccatctt
      781 ctcgcccacc ccgatgaact tcaccgggat cctgaactga ttggcgatgg ccagcacgac
      841 cccgcccttc gccgtcccat ccaacttggt aatagccagc gacgtcacct ccgtggcggc
      901 agtaaattgc cgggcctgct ccagggcatt ctgccccgtc gatccatcca gcaccagcaa
      961 cacctcatgc ggcgcatccg gcatgatctt cttcaccacc cggctgatct tgctcaactc
     1021 ttccatcagg taggccttgt tgtgcagccg gcccgccgta tcgatgatca cgacatcggc
     1081 gccccgcgca accccgctct gtaccgtatc aaaggccacg gcgccgggat cggcccccat
     1141 accctgcttg atgatcggta ccccaacccg ctcgctccag atggtcaatt ggtccaccgc
     1201 cgccgcccgg aacgtatccg ccgcccccag caataccgat ttccccgcct tcttgaaatt
     1261 atacgcgagc ttcccgatgg tcgtcgtctt cccgacccca ttcaccccaa ccaccaggat
     1321 cacatagggc ttgctccccg acggcgtact gaaatcccgg tacgactgct ccttgggctc
     1381 taccaggatg ttctcgatct cttcttgcag taaaccattt aactcactgg tattaatata
     1441 tttatctctt gctacccgct tttcgatctg tttgatgatc tgtacggtcg tatcgatccc
     1501 gacatccgcg ctgaccaggg cgtcctccag gttgtccagc acttcttcat cgactgtgct
     1561 cttcccggca accgccttcg taagccgggt aaggaaacct tccttggtct tctgcaaccc
     1621 ctggttcagg ctttcctttt ccttttttcc aaaaagttta tcaaatagtc ccatagaatg
     1681 aatttcggca aaaaattgct ttcggcaaca tatgcaaaaa gctgtctcgc agagacagct
     1741 tttccattat cttactaaac cctatcaatt acttttgagc cagatactcc ttgatcttct
     1801 ccttgtgcac gatcgcttcc ttgaaggtat aagcgccggt cttggggctg cgaacagcct
     1861 tgatcacttt tgtccagttc ttggcctccg cggcagcctt ggcatccttg gttttaatag
     1921 cggtttttgc tgcttttgcc atggttattt aatttcttta tgaacagtta ctttctttaa
     1981 aatgggattg aacttcttca gctccagacg ttccggagtg ttcttcttgt tcttggtagt
     2041 gatataccgg ctggtccccg gctttccgct ggtcttatgc tccgtgcatt ccagtatcac
     2101 ttgaaccctg cttcctttct ttgccataat cgggtgaggt tttaaaaatg attagatctt
     2161 ttcgccgttc gctcttaatt tcttgacaac gctgtacaga ccgtttttgt tgatcgtccg
     2221 cagcccttcc gaggagacct tcagtgtgat ccacttgtct tcctcagcca ggaaaaagcg
     2281 tttggtctgc aaattgggta agaacctgcg ctttgtcttg atattagaat gcgatacgct
     2341 atttccggaa accggaacct tacccgttat ctgacaaact ctagccatga ctgaaaattt
     2401 attaggagtg caaaggtcgg taaatccggg caattatcaa aaagaaatcc cttttttttc
     2461 aacgacttgt ccacccccgt ggaaaaccat tccttggcag tttccggccg ccggcatggg
     2521 caggcctcat ttcacttcga aggctatcac ataatcccct tgtttggttt tcaacttccc
     2581 atgccccccg gcggcgatca ccacatattg tttaccatca agcgaatagg tcatcggcgt
     2641 cgcctgtccc cccgccggca gcgcatactc ccaaagcatc gcacccgtcc ggcggtcgaa
     2701 tgcccggaaa tgcccatcca ggcttccc
//
LOCUS       DATGFG010000940         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1308680, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000940 DATGFG010000000
VERSION     DATGFG010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308680"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VKQ52_17770"
     CDS             <1..1035
                     /locus_tag="VKQ52_17770"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ89111.1"
                     /translation="LTFAFHARVYLPDAPAGITFATALQASLFELGCEMALTYGTVYW
                     LFPLYFERKKYVRFALGSGALLVLIFLLTSPNWMDFHFPGYSHDGFSSFWNTLMNFIR
                     MSFTTWLLFIAWRMFKQHFQRLNERATLSRETADVEFQLLKAQVHPHFLFNTLNNIYS
                     FALDGSPRAGLLLSQLTGMMRYMIYDCEADRMALDRELRLLEDYIGLEKARYGPRLDL
                     QVSIEGETHRRQIAPLLMIPFIENCFKHGASQVLDRAWVRLQIRARDNQLDFRLSNNK
                     PPAGAALNGRSGIGLKNIRQRLELLYPHRYRLDIESAGDTFSVRLVVPLEEDASGLHK
                     NVQETRPPKM"
     gene            1211..2074
                     /locus_tag="VKQ52_17775"
     CDS             1211..2074
                     /locus_tag="VKQ52_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ89112.1"
                     /translation="MANERKITCLVVDDEPPAIDILRKYIGAVATLDLAGTCNNAVEA
                     LNVLQARNIDLLFLDIQMPQLLGTDLIRTLKKPPRVIFTTAHRKFAIEGYELDAVDYL
                     LKPISFERFLKAVNKVLDGDAPPAPMGPANRSTEPDASTRAVPGSPVHHSPGSPAHHS
                     SGTPAHLSPGSSTHHSPGSPARNDAFLHLRADRKTVQIALDDILYIESIKDYIKVVTR
                     SKTIVSKQSLSTLEDTLPRESFIRIHRSYIVAISKIESYTSELVEIARQELPISRMYR
                     LDVEKILQRRP"
     gene            2640..>2930
                     /locus_tag="VKQ52_17780"
     CDS             2640..>2930
                     /locus_tag="VKQ52_17780"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLZ89113.1"
                     /translation="MKITNILGIDVSKSTLDCHLYLQHQQLDSIPNSVQGFKQLNKWL
                     KKQCKKELRELLVVMEHTGIYTYGLEQYLHQQGIAYVKRPALDIKRSLGMIRG"
BASE COUNT          665 a          909 c          752 g          604 t
ORIGIN      
        1 ctcacctttg cgtttcacgc ccgggtctac ctgcccgacg ccccggccgg gatcaccttt
       61 gccaccgcgc tgcaagcatc gctgttcgag ctgggctgcg agatggcgct cacctatggc
      121 accgtttatt ggctgttccc cctgtacttc gagcgaaaga aatatgtccg cttcgccctg
      181 ggcagcggcg cgctgctggt cctcatcttc ctcctcacct ccccgaactg gatggatttc
      241 catttcccgg gctatagcca cgacggcttt tcttcgttct ggaacaccct catgaacttt
      301 atccggatga gttttaccac gtggttgctg ttcattgcct ggcgcatgtt caaacagcat
      361 ttccagcggc tgaacgagcg ggctaccctc agcagggaaa ccgccgacgt cgaattccag
      421 ctgctgaagg cgcaggtgca cccgcatttc ctgttcaata ccctgaacaa tatctactcc
      481 tttgccctcg acgggtcgcc ccgggccggc ctcctcctgt cccagctcac ggggatgatg
      541 cggtatatga tctatgattg cgaggccgac cggatggcgc tggaccgcga gctgcggctg
      601 ctcgaagact atataggatt ggaaaaggcc agatacgggc cccgcctgga tttgcaggtc
      661 agcatcgaag gcgaaacgca tcgccggcag atcgcccccc tgctgatgat cccgtttatc
      721 gagaactgct tcaagcatgg ggccagccag gtgctcgacc gggcctgggt gcggctacag
      781 atccgcgcca gggataacca gctggatttc aggctgagca ataacaaacc acccgccggc
      841 gcggccctca acggaaggag cggcatcggg ctgaagaata tccggcagcg cctggaactc
      901 ctgtatcccc accgctaccg gctggatatc gaatccgcgg gtgatacttt ttccgttcgc
      961 ctggtcgtac ctttggaaga agatgcctcc gggctccaca agaatgtgca ggaaacgcgc
     1021 ccaccgaaaa tgtagcgcat ttcccgcaaa ccgctaatgt ctgtagaact tcgcccccca
     1081 acactgttgg ggcgctcatt cgcggccttc gcaatgctcg gccgtggaca agatgcctcc
     1141 ggcatcttaa cgtgcaggaa acgcgcccac cgaaaatgta ggaaacgcgc tcttcgaaaa
     1201 tttgatgccc atggccaatg aaagaaaaat aacctgcctg gtagtcgatg acgagccgcc
     1261 ggcgatcgac atcctccgga aatatatcgg ggccgtagct accctcgatc tggcgggcac
     1321 ctgcaacaac gccgtcgagg cgctcaatgt cctgcaggca aggaatatcg atctcctctt
     1381 cctcgacatc cagatgccgc agctgctcgg caccgacctc atccgcacgc tcaagaaacc
     1441 gccccgggtc atcttcacca ccgcgcaccg caaattcgcg atcgaaggtt acgaactcga
     1501 cgccgtggat tatctgttga aaccgatctc cttcgagcga ttcttaaagg ccgtgaacaa
     1561 ggtgttggac ggtgacgccc ctcctgctcc aatgggaccc gcgaaccggt ccacggaacc
     1621 cgatgcatcg acacgcgccg ttcccggatc gcccgttcac cactcccccg gttcaccggc
     1681 tcaccactcg tccggaacac cggcccatct ctctcctggc tcatccactc accacagccc
     1741 cggatcgccc gcccgcaacg acgccttcct ccatctgcga gccgaccgca agaccgtcca
     1801 gatcgccctc gacgacatcc tctacatcga aagcatcaag gactacatca aggtcgtcac
     1861 gcgcagcaag accatcgtca gcaaacaatc cctctccacg ctcgaggaca ccctgccgcg
     1921 ggaatccttt atccgcatcc accgcagcta tatcgtggcg atcagcaaga tcgaatccta
     1981 taccagcgag ctcgtcgaga tcgcccgcca ggaactcccc atcagccgca tgtaccggct
     2041 cgatgtcgag aagatcctgc aacggcgacc ctgaccgagc acagcgaggt cggccgaagg
     2101 cccatcatag gcccttgcag atacccgagc ggcagcgaag ggtacgaacg aggagaagcg
     2161 tgtcgaagca gcttcgacga agttctaagg catcggtacg gacagggcgg aagtcagcgg
     2221 agcgggggga ccggcggcgg cctgaacggt ggtcattcgg caaccagcat ccccaacctc
     2281 gaactacggg cattcggcaa ccagcctccc caacccgatt tcacttcgtt gcgaccttta
     2341 gtttcgcact ataaacattg cctgaaaaaa gactggaagg tgaactatcg tttgcgaccg
     2401 gaggatcggc tccctggagc gtattagacc ttcttttttt accctaatgg tttgaggata
     2461 aatacatttg aaaagaagag aggtgaacta agagtgcgac tggaattaac ggttccgcgt
     2521 tgcgtattag acctctcttc ctttctggtt gcctcgcgca agttagaata tcagccgtgg
     2581 tgggctatta aaggtcttag cagcagggca acactttttt tattcctaaa agtaaagtta
     2641 tgaagatcac caacatcctg ggtattgatg tgtcaaaaag caccttagat tgtcatttgt
     2701 accttcagca tcaacaactt gattccatac ccaactccgt ccaggggttt aaacagctca
     2761 acaaatggct gaaaaagcaa tgtaaaaaag agttacggga acttctggtg gtcatggagc
     2821 atactggcat ctatacctat gggttggagc agtatttaca ccagcagggt attgcctatg
     2881 tcaagcgacc tgctctcgac atcaaacgct ccctggggat gatccgggga
//
LOCUS       DATGFG010000941         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_619154, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000941 DATGFG010000000
VERSION     DATGFG010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619154"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..>3492
                     /locus_tag="VKQ52_17785"
     CDS             179..>3492
                     /locus_tag="VKQ52_17785"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLZ89114.1"
                     /translation="MESSTLIKLLSDRRQLAGVGITFIEGSDQERFLSYDQLYHSALK
                     VLSVLQRKGMQDGDELVIQIQDNRTFIIAFWACLLGGIVPVPLSVGQNDNHREKVYTI
                     WPVLKNPHLITAEDNFTRLGAFARSNGFERTFSGIADKTVKEPEIWLSSSEGKVFDAK
                     PDDIAFVQFSSGSTGNPRGVMLTHRNLLTNIGAIERAARYTKSDSTISWMPLTHDMGL
                     IGFHLNPLFSKINQFLIPTANFIRRPALWLDKVNEHRVTILCSPNFGYKYLLKHIQPD
                     TGKNWDLSCVRVLYNGAEPISGKLCREFLARMAVHGLPGNAMCPVYGLAEASLAVTVS
                     GLDDEVMSINADRNKLNAGDRITSVWEEDKNAVAIVNVGRVVADCILRIADDDDKPVE
                     KDVIGHIQIKGENVTAGYYNNKAETRKIITGDGWLRTGDLGFIKEGSLYITGRAKDIF
                     FVNGQNFYPHDIEQLAQEVEGIELNKIVVAGLPADDLQTEEVIAFILHRGSLADLLPL
                     MASLKEHINSKAGIELDRIIPVKDIPRTTSGKLQRYKLIEQLRKGDFADIGKKVQALT
                     EAAGQIAPAPSAAAAGNEQILLQIWQRVLGTGNIGVTQRFFEIGGNSLKAAEVAMILS
                     KEFQTELPMELLYEKQTIRELASEIGFPGKGGYSPIPEGPRNECHPLSWAQKRIYYFA
                     ELNKTSIAYNVPVALRIRGHVNVGRLENNINELIARHDVLRMSFPIAGEPVFRVNEDI
                     KVRVDRIACDEDRIDEELRAQVRPFNLAIAPLFSVRLLELKGKEEYILFLDFHHIIAD
                     GMSVYQFIDELFGSYSGNRLPALSVDYKDYVYWEKDSEQSKEIGRQQAFWLGELQGDL
                     QLLDMPADFSRGAILSTEGAKLAFQINGETVTRLKQVAAANTCTLHVLLFSLYAVLLS
                     KLTGQREVIIGVPVAGRRHPDLQKMIGMFVNNLAVKSSIDGDETLVQLLARQKEKMLA
                     AFANQEYPFEQLIQDLGPTPDSSRNPVFDSMFIYQNMGFPDISTANLGVSPYFFDPGI
                     SKFDISMEVFERGESISYYIEYPTKLFKRETILRFAEYFDNLIGHAAGNSREKLADWS
                     LMNERTYFRCVTAFNAT"
BASE COUNT          938 a          778 c          937 g          839 t
ORIGIN      
        1 ctccggcgga gtgtgatggc tggaagagac ataccaggca gctttgtacg ctgtattatt
       61 ttgaaggagg tcattttttc ctccatgatg aaacggaaca gatggtgaag atcatcaata
      121 ctacccttgc caacgtgaat acagctgcgg ccgtaaagct cgcaacaagc tattgatcat
      181 ggaaagttcg actcttataa agctattgtc cgacagaagg cagctggccg gcgtcgggat
      241 tacttttata gaaggaagtg accaggagcg tttcctttcc tatgaccaat tgtaccattc
      301 ggcgctgaaa gtgttatcgg tacttcagcg taagggaatg caggacggag atgaactggt
      361 gatccagatc caggataacc ggacctttat catcgctttt tgggcatgtc tgttgggcgg
      421 catcgttccc gttcccttgt cggtaggcca gaatgacaat catcgggaaa aagtatatac
      481 tatctggccg gtgttgaaaa accctcacct gatcactgcc gaggataatt ttacccggct
      541 gggtgcattt gcccggtcaa acggctttga aagaaccttt tccgggatag ccgataagac
      601 cgtcaaagag ccggaaatat ggctctcctc atcagaaggg aaggtgttcg atgcaaagcc
      661 ggacgatatt gcctttgtcc agttctcatc gggttctacg ggaaatccca ggggagtcat
      721 gcttacgcac aggaacctgc tgacgaatat cggggcaatc gaacgggcag cccgttacac
      781 gaagagtgac agcaccatta gctggatgcc gttaacccat gatatggggt tgataggttt
      841 ccatctgaac cccttattca gtaagataaa tcagttcctg atacccacgg ctaattttat
      901 ccgccgcccc gccctttggc tggacaaggt gaatgaacac agggttacca ttttatgctc
      961 tcccaatttc gggtataaat atctcttaaa acatattcag ccggacaccg gcaagaactg
     1021 ggatttgtcg tgtgtaaggg tgctttataa tggagccgaa cccatatccg ggaaattatg
     1081 ccgggaattc cttgcccgga tggccgttca tgggttgcca gggaatgcga tgtgcccggt
     1141 ttacgggtta gccgaggcca gcctggccgt aacggtctcg ggcctcgatg acgaggtgat
     1201 gtccataaat gccgatagga acaagttaaa tgccggcgac aggataacat ccgtatggga
     1261 ggaggataaa aatgccgttg cgatcgtcaa tgtagggcgg gtggttgccg attgtattct
     1321 aagaattgcc gacgatgacg acaagccggt tgaaaaggat gtaatcggcc atatccagat
     1381 aaaaggagag aatgtgaccg ccgggtacta taataacaag gcggaaacaa gaaaaataat
     1441 caccggggat ggctggttac gaacgggaga cctgggcttt ataaaggaag gatctttgta
     1501 tattaccggc agggccaagg acatattttt tgttaatgga cagaattttt acccgcatga
     1561 tatcgagcaa ttggcccagg aagtagaagg tatagagctg aacaagatag tggtagccgg
     1621 tttgcctgcc gatgatctgc agacagagga agtgatcgct tttattcttc acaggggaag
     1681 ccttgccgat cttttaccgc ttatggcctc tttaaaggaa catatcaata gtaaggccgg
     1741 catcgagctc gacaggatca ttcctgtaaa agatataccg agaactacga gcggcaagtt
     1801 acagcgatat aaattgatcg aacagctcag gaagggagat ttcgccgaca tcgggaaaaa
     1861 agtacaggca ttgacggaag ccgccggaca gattgcaccg gcaccgtccg cagctgcagc
     1921 gggcaatgaa caaatcttat tacaaatatg gcagcgggtg ctgggaacgg ggaatatcgg
     1981 cgttacccag cgtttctttg aaatcggcgg gaattcctta aaggccgcgg aggtggcgat
     2041 gatcctctcg aaagaatttc agaccgagct gccgatggag ttgttatatg agaaacaaac
     2101 catcagggag cttgcttctg agatcgggtt tcctggaaaa ggaggttaca gccccattcc
     2161 tgagggcccg cggaacgagt gtcatcccct gtcgtgggcg caaaaaagga tctattattt
     2221 cgcagaactg aataaaacat cgatagccta taatgtgcct gttgccttgc gtatccgggg
     2281 gcacgtaaac gtcggcaggc ttgagaacaa tatcaacgaa ttgatcgccc gtcatgacgt
     2341 attacgaatg tcgttcccga tagcagggga gccggtattc cgggtaaatg aggatataaa
     2401 ggtccgggtg gatcgcatcg catgtgatga agacaggatc gacgaggagt tgcgagccca
     2461 ggtacgacca ttcaaccttg cgatcgcgcc gcttttcagc gttcggctgc tggagctgaa
     2521 agggaaggaa gaatacatat tgttccttga ttttcatcat ataatcgccg atggaatgtc
     2581 ggtttatcag tttatcgatg agctgttcgg gtcttattcc gggaaccggc tcccagcctt
     2641 atctgtcgac tacaaggact atgtttactg ggaaaaagat agcgaacaaa gtaaagagat
     2701 cggccggcaa caggcctttt ggcttggtga acttcagggg gacctgcaat tactggatat
     2761 gcccgccgac ttctcccgcg gtgcgatatt gagtaccgaa ggggcgaagc tggcttttca
     2821 gataaacgga gaaaccgtta caagactaaa acaggtggca gcggctaata cctgcaccct
     2881 tcatgtattg ctctttagcc tctatgcggt gcttctttcc aagctcacag ggcagcggga
     2941 agttattatc ggtgttccgg tggccgggcg aaggcacccc gacctgcaga aaatgatcgg
     3001 catgtttgtc aacaatcttg cggtaaagag cagtattgat ggtgatgaaa cgcttgtcca
     3061 actgctggca agacaaaaag agaaaatgct ggcagcattt gccaaccagg agtatccttt
     3121 tgaacaactc atacaggatc ttggaccgac ccctgacagc agcaggaacc cggtatttga
     3181 ttccatgttc atctaccaga acatgggttt cccggatatc agcaccgcaa accttggcgt
     3241 ttcaccttat tttttcgatc ccggtatttc caaattcgat atttctatgg aagtattcga
     3301 acggggggaa tcgatatcgt attatataga atacccgacg aagctcttca aaagagaaac
     3361 catactgagg ttcgcggaat actttgacaa tcttatcggt catgctgccg gcaacagccg
     3421 ggaaaagctg gccgactggt cattgatgaa tgaaagaacc tatttccggt gtgtaacggc
     3481 atttaatgca ac
//
LOCUS       DATGFG010000942         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1305137, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000942 DATGFG010000000
VERSION     DATGFG010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305137"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VKQ52_17790"
     CDS             <1..691
                     /locus_tag="VKQ52_17790"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HLZ89115.1"
                     /translation="RPLLLRNIHEARLSADEKHYLNVLRNWDLSNDPNEEGPVIFQYW
                     WSHLTHDIYDDEFSKSSLNMMRPFESTLLEALLRDSAYSFIDNVNTPEKENLGQVVTT
                     AFLQAIPDLTAAAGEDRLTWAKNKDTWVRHLLRIPELSRTHLPIGGGVHCINAAKKLH
                     GPSWRMIVQLTTPTEAYGIYPGGQSGNPGSPYYDNFVDKWAAGKYNALWVMAPEEAKD
                     RRVKWALHFSN"
     gene            704..1054
                     /locus_tag="VKQ52_17795"
     CDS             704..1054
                     /locus_tag="VKQ52_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89116.1"
                     /translation="MIKFFVSVLLIALLSFICGLFLPWWTIAVAAFVVSALIPQRPWV
                     SFFAGFIALFLLWGGLAWWIDYANNGILSARIAGLLKLQGSSVLLVLVTAFIGALAGG
                     GGSLTASYLYAGKK"
     gene            complement(1213..>2746)
                     /locus_tag="VKQ52_17800"
     CDS             complement(1213..>2746)
                     /locus_tag="VKQ52_17800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HLZ89117.1"
                     /translation="IGIVYMDMKDYPRAVDYLRRALNIARHFPDKSGIAIGYINLSEA
                     FAANNQLDTAIAYSKAALVIAETINDTISLSASLDDLGEYSTRNGHPREGLAWLQRAL
                     LVSQNAHDDNGVASVHLSMAAAWSALQEYRQSIALAGTGLQEARKLHANEYSKTAYHE
                     LYTAYAGLKEFDKALDYRNREIALNDSIYNLEKEKQVRGLQVTYELEKKQHQLDLSNR
                     DRLLQQEEMSRDRIRHYVLAAGIVLLTLWAILLIRSNTRRKQLNRLLESRNKEIEKKN
                     HQLEDLNAVKNKLLSIIGHDLRSPVATLRGFVDLLRQSALTPDQIHYFSQKMGESLES
                     TSRLLENLLFWAKSQMEGMEVNAKPFDILPVIEQNGRLVRDRATEKKINLVIGGPDKP
                     TSPLMVFADEVMVDMVIRNLVENALKFSRADDTVAITATAAGNRVAITIRDTGQGIPL
                     ENQEKIFRTITYTTTGTSREKGSGLGLSLCKELVEKNGGKIGFASEPGNGTAFTFTLP
                     IP"
BASE COUNT          479 a          658 c          917 g          692 t
ORIGIN      
        1 gcggccgttg ttgctccgga atatccacga ggcgcggctt agcgcggatg agaagcatta
       61 cctgaatgtg ctccgcaact gggatctcag caatgacccg aatgaggagg ggccggtgat
      121 cttccagtac tggtggagcc atcttaccca tgatatttac gacgacgagt tctcgaagtc
      181 gagcctgaat atgatgcggc ctttcgagag cacgctgctg gaggccctgt tgcgggactc
      241 cgcgtattcc tttatcgaca acgtgaatac ccccgagaag gagaatctgg ggcaggtggt
      301 caccacggcc tttttgcagg ccattcccga cctgacagcc gccgccgggg aggacaggct
      361 cacctgggcg aagaataagg atacctgggt ccgtcatctg ttgcgaattc cggagctgag
      421 ccggactcat ctgccgattg gcgggggcgt ccattgtatc aatgccgcga agaaattgca
      481 tggtccgagc tggcggatga tcgtgcagct cacgaccccc accgaggcct acgggatcta
      541 tcccggcggg cagagcggga atcccggtag tccttattat gataattttg tcgacaagtg
      601 ggcggcgggc aagtataatg ccctttgggt gatggcgccg gaagaggcga aggaccggcg
      661 ggtgaagtgg gccctgcatt tttccaatta aacataggtg atcatgataa aattctttgt
      721 gtcggtgttg cttatcgccc tgcttagctt tatctgcggg ctttttcttc cctggtggac
      781 gatcgccgtg gcggcctttg tcgtcagcgc actgatcccg cagcggccct gggtttcttt
      841 ttttgccggg tttatcgcgc ttttccttct ttggggcggg ctggcgtggt ggatcgacta
      901 tgccaataac ggtatcctgt cggcccggat cgcgggtctt ctcaagttgc aggggtcatc
      961 ggtgttgctg gtgctggtga cggcttttat aggggcgctg gcgggtgggg gtggatcgct
     1021 tacggcgagt tatttgtatg caggcaaaaa atgagggaaa aatagttttt tgagctccgt
     1081 tcaggggatg ggtgagtgag gctggttcag gggatgagta gctgaggccg gtgcagggga
     1141 tggataagtg agggccagat caggggatgg gcaaatgaga ccccgtccag ggatggataa
     1201 gtgagggttt ggtcagggga tgggcagcgt gaaggtgaag gccgtgccat tgccgggttc
     1261 actggcgaaa cctatttttc cgccattctt ttccaccaat tccttgcaga gggacaggcc
     1321 cagtccgctg cctttttccc gtgatgttcc cgtggttgta taggtgatcg tccggaagat
     1381 cttttcctgg ttttccagcg gaatcccctg gccggtatcc cggatggtga tggctaccct
     1441 gttgccggcg gcggtggcgg tgatggcgac ggtatcgtcg gcgcgggaga atttgagtgc
     1501 gttctcgacg aggttgcgga tgaccatatc gaccatcact tcgtcggcga agaccatcag
     1561 cggggaagtt ggtttatcgg ggccgccgat gacgagattg atcttctttt ctgtggcgcg
     1621 gtccctcacc aggcggccgt tctgctcgat aacggggagg atatcgaaag gttttgcgtt
     1681 cacctccatt ccttccatct ggcttttagc ccagaacagg aggttttcca gcagccggga
     1741 ggtgctttcg aggctttccc ccattttctg gctgaagtag tggatctggt cgggggtgag
     1801 ggcggattgg cggaggaggt cgacaaagcc tctgagggtg gccacgggac tccgcagatc
     1861 gtgaccgatg atggagagca gcttattctt gaccgcgttg aggtcttcca gctggtggtt
     1921 ctttttctct atttccttat tgcggctttc gagtaaccgg ttcaactgtt tgcgccgggt
     1981 gttgcttctt atcaggagga tggcccacag ggtgagcagg acgatgccgg cggcgaggac
     2041 gtaatggcgt atcctgtcgc gggacatctc ttcctgctgg aggagccgat ctctgttgct
     2101 gaggtcgagc tgatgttgtt tcttttccag ctcgtaggtg acctggaggc ccctgacctg
     2161 cttctctttt tcgaggttgt agatactatc gttgagggcg atctcgcggt tgcggtagtc
     2221 gagggccttg tcgaattcct ttagtccggc gtaggcggtg tataattcat ggtaggccgt
     2281 tttgctatat tcgtttgcgt ggagtttgcg cgcttcctgc aggcctgtcc cggccagggc
     2341 gatgctctgc cggtattcct gcagggcgga ccaggcggcg gccatggaaa gatgtaccga
     2401 ggcaaccccg ttgtcgtcgt gtgcattttg agagacaagg agtgcgcgtt ggagccaggc
     2461 caggccttcg cggggatggc cgttgcgggt ggagtactcg ccgagatcgt caaggctggc
     2521 ggaaagggag atcgtgtcgt tgatcgtctc tgcgatgacc agggcggctt tgctataggc
     2581 gatggccgtg tcgagctggt tgttggcggc gaaggcctcg ctgagattga tatagccgat
     2641 ggctatgcca gacttgtcgg ggaaatggcg ggctatgttc agggcgcggc gcaggtaatc
     2701 cactgccctg ggatagtcct tcatatccat gtagacgatg ccgatg
//
LOCUS       DATGFG010000943         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1076848, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000943 DATGFG010000000
VERSION     DATGFG010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076848"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VKQ52_17805"
     CDS             complement(<1..1089)
                     /locus_tag="VKQ52_17805"
                     /inference="COORDINATES: protein motif:HMM:NF025651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YWFCY domain-containing protein"
                     /protein_id="HLZ89118.1"
                     /translation="MSGSTGSDENSLHRILDTIRIAGIGVLLLHFYLHTHPRDPWPAD
                     AIGQRLTDAVANLPVVGDFLAGKSLAFLLLAVSLIGANGKKSPGHTVRSGIAFIFAGV
                     VLFFGSGLFAGAALPYILFCSAGWLLTLYGGNYMSRVIWRKAEEDIFNRLHESFPQER
                     RLLQNSRSVNLPAHYQYNGAVLESWVNFLAPQRGTLILGSPGSGKTAYVLQHFIRQGV
                     QQGHAMVIHDFKYDDLSRLAYNCFLRHKKRCPGNLAFYNIQFDDLDRSNRCNLLHPST
                     LNDISDAEESARALLLGLNMDWIAKQGDFFVESAVTFVKMLIWYLRLYKDGIYCTWPH
                     VIELAQTPSKKLFTLLRVEPQLQTHVAPF"
     gene            complement(1086..2204)
                     /locus_tag="VKQ52_17810"
     CDS             complement(1086..2204)
                     /locus_tag="VKQ52_17810"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HLZ89119.1"
                     /translation="MLARTKPFAKVATIMYYNEQKVKAGQAQCIYAGNFLPDAADLTP
                     GDKRRRFSDVLQLNQRVVNTAMHIVLQFPPGEDLDNDRLIAITASYMQRIGYGDQPYL
                     VYRHSDTASPHLHIVTITIRPDGSHIRTPFLGPRISEPARKAVEIEFGLTKASGHKRK
                     AIERPGPAQKIQYGKLPSEEAIANTLSYILETFRYRSIAELNAILRLYNLTAKTGRPG
                     SRLHTFGGLLYQILDEKGQGRGAPVKASSLPFKPTLSWLETKFAEHRLLPPATVNRTR
                     FAFDSVLRENPADGAAFRDALRRNQLAIAGEHPSDGSPTGLLLVNLAEKTVIPASELG
                     AAYDLPALRQKLSFDPLQISITETRKQGRQRKQQKIHL"
     gene            complement(2171..2614)
                     /locus_tag="VKQ52_17815"
     CDS             complement(2171..2614)
                     /locus_tag="VKQ52_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89120.1"
                     /translation="MGIGSEEEEKIMKRRKELRNKTLAVYVTATELAALKEEFKSTTH
                     RVFAEFIRARLREEPLVRKTRNQSLDNLHHGLIGIKAELETARRSFTEAVNRFKSLSP
                     DLVDQQCLEFLLAEEIDLRETIKAVQSTLIQLYERCSQEQSPLQK"
     gene            2901..>3326
                     /locus_tag="VKQ52_17820"
     CDS             2901..>3326
                     /locus_tag="VKQ52_17820"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLZ89121.1"
                     /translation="MLHEETVATGTLELIRKLSQDSMLQDFVLVGGTALALQLGHRKS
                     VDIDLFASRDIEAGAIAKHLEKSYNAKIDNVTKNGVFGWIDGVKTSLIAHHHDMIAPL
                     VTTGDVRMASLDDIGAMKMHAITNSGQRAKDFIDMYYLLE"
BASE COUNT          731 a          804 c         1036 g          755 t
ORIGIN      
        1 aaagggcgcg acatgggtct gtaactgggg ttcgacccgg agaagggtaa agagtttctt
       61 gctcggggtc tgggccagct cgataacatg gggccaggtg cagtagatgc cgtccttgta
      121 cagccgtaga taccagatca gcatcttgac aaaggtgacg gccgattcca cgaagaaatc
      181 gccctgtttg gcgatccaat ccatgttgag gccaagcagg agcgccctgg cggactcctc
      241 ggcgtcgctg atgtcgttga gcgtcgaagg atggagcagg ttgcagcggt tagaacgatc
      301 gagatcgtcg aactggatgt tgtagaaggc gagattgccg ggacaccgct ttttgtgacg
      361 gaggaaacaa ttgtaggcca gccgcgagag atcatcgtat ttgaagtcgt ggatcaccat
      421 tgcatgaccc tgttgaacgc cctgccggat gaaatgctgc aggacatagg ccgtcttccc
      481 cgatccgggt gagcccagga taagtgttcc gcgctggggg gcgaggaagt tcacccagga
      541 ttccaggaca gcgccattgt attgataatg ggcaggcaga ttgacggacc gcgaattttg
      601 gagaagacgc cgttcctggg ggaaggattc gtggagccgg ttgaagatat cttcttcggc
      661 tttccgccag atgacccggc tcatgtagtt accgccatag agggtgagga gccaaccggc
      721 cgaacaaaac aggatatagg gtagggcggc gccggcgaag agtccgcttc caaagaagag
      781 tacgacacca gcaaaaatga aagctatgcc gctacgcacc gtatggccgg gtgatttttt
      841 accattggcg ccaatcaggg aaacggccag cagcaggaat gcgagggact tacccgcaag
      901 gaagtcgccc acgacgggaa ggttggctac tgcgtcggta agccgttgcc cgatagcgtc
      961 ggcaggccag ggatcccggg gatgggtgtg caaatagaaa tgcaggagca ggacgccgat
     1021 gcccgcgatg cgaatggtgt cgagaatgcg gtgcaggctg ttctcatcgc taccggtgga
     1081 gccgctcata aatggatctt ttgctgttta cgttgccggc cctgcttccg ggtttcggtg
     1141 atgctgatct gaagcggatc gaaggaaagc ttttgacgaa gggcgggaag gtcataggca
     1201 gcgccaagct ccgatgccgg tatgaccgtt ttttcggcaa ggttgaccag caggagaccg
     1261 gtgggtgacc catcggaagg gtgctccccg gcgatggcga gctggttccg gcgaagggca
     1321 tcccggaagg cggccccgtc cgcggggttt tcgcggagga cgctgtcgaa tgcgaagcgg
     1381 gttcggttga ccgttgccgg cgggagaagc cggtgttccg cgaattttgt ctccagccaa
     1441 ctcagggtag gcttgaaggg aagggaactg gcttttacgg gagcgcccct gccttgccct
     1501 ttctcatcca ggatctggta gagtaatcca ccgaaggtat ggagccggct gccgggacgc
     1561 ccggtcttgg ccgtcaggtt atacaggcgg aggatagcat tcaactcggc gatagaacgg
     1621 tagcggaacg tttccaggat ataagaaagc gtgttggcga tggcctcctc ggagggaagc
     1681 tttccatatt ggatcttttg ggcggggccc ggacgctcga tggctttccg tttgtggccg
     1741 ctcgcttttg tcagcccgaa ttctatttcc acggccttgc gggccggttc ggatatccgg
     1801 ggtcccagga acggggtgcg gatatgactt ccgtccggcc ggatcgtgat ggtaacaata
     1861 tgaagatgcg ggctggcggt atccgaatgc cgatagacca ggtagggctg gtcgccatag
     1921 ccgatccgct gcatatacga tgcggtgatg gcgatcaacc ggtcattgtc caggtcttcg
     1981 ccgggtggga actgaagcac gatgtgcatg gcggtgttga ctacgcgctg gttgagctga
     2041 agcacatcgc tgaagcgccg gcgtttgtcg ccgggggtca gatcggcggc atcgggcagg
     2101 aaattaccgg cgtagatgca ttgcgcctgc ccggccttga ccttttgttc gttgtagtac
     2161 atgatcgtgg ctacttttgc aaagggcttt gttcttgcga gcatctttcg taaagttgaa
     2221 tgagagtgga ttgtacagcc tttatcgtct cgcggagatc tatttcttcc gccagcagga
     2281 actcgaggca ctgctgatcg accaggtccg gcgacagact tttgaaccgg ttcacggctt
     2341 ccgtgaagct gcgccgggcg gtttccagtt ctgccttgat gccgatcagg ccatgatgaa
     2401 gattgtcgag ggactggttg cgggtcttgc gtacaagagg ctcttcgcgg aggcgggccc
     2461 ggatgaattc ggcgaacacc cggtgggtgg tcgacttgaa ttcctctttc agggccgcga
     2521 gttccgtcgc agtaacatag acggccaggg tcttatttct caattccttc cttcgcttca
     2581 tgatcttctc ctcctcctcg gaacctatac ccacaccccc aagggcaatg gaaatccctt
     2641 attttgatct taaagttagc ataacggcat cgaatatcgc ctgatgagtt tttttagctg
     2701 ttcaggccgg ggaggtggtt tcgcgaatga tcgtttttca ccttgtatag gagcccagtt
     2761 tgctctttca agcgagttga tccggccctg gtagaagttt atcagccgga agatcccgaa
     2821 ttaatcgaca taacttatac ataagttctg agatcagcgc ctttccgtca tgcaaatttg
     2881 cctgataaac aaggaagaaa atgttacacg aggagaccgt agccactgga acgcttgaat
     2941 tgataagaaa gttatcgcag gacagcatgt tacaggattt tgttttggtc ggcgggacag
     3001 cgctggcgct gcagctcggg catagaaagt cagtagatat agatctattc gcctcgagag
     3061 atatcgaggc cggagcgata gccaaacacc tggagaaatc ctataacgca aagatcgata
     3121 atgttacgaa gaatggtgta ttcgggtgga tcgatggtgt caaaacaagc ctgatcgctc
     3181 accatcacga catgatcgcg cctctcgtta cgaccgggga cgtcagaatg gcatctttag
     3241 atgatattgg ggccatgaaa atgcacgcaa tcacgaatag cggccagcgt gcaaaagact
     3301 ttatagacat gtattacctt ttggag
//
LOCUS       DATGFG010000944         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_637930, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000944 DATGFG010000000
VERSION     DATGFG010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637930"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VKQ52_17825"
     CDS             complement(<1..1543)
                     /locus_tag="VKQ52_17825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLZ89122.1"
                     /translation="MRFNKSTTILLIAVFAALLFLPFLGRVHLFDWDEINFAECSREM
                     MKLRDYTRVYVDFKPFWEKPPMFFWMQSTAMHLFGVNEFAARFPNALCGIVTLIVVFI
                     CGSRIYDQKFGLLWALAYGGSLFPNMYFKSGIIDPWFNLFTFLSLYNFILYHWKRNGF
                     DKPGAQKSQFYYVVWSGIFMGLAVLTKGQVALMVFMLALGVYFLYNRFRFFFSWWHAL
                     LFLLVAFLVTFTWYGYETIKRGPWFINEFLKYQWRLFTTHDADQAGFFGYHYIVLLVG
                     CFPASILAIPSFFRAKYNGRWDRDFKTWMQILFWVVTVLFTIVQSRIIHYSSMAWFPV
                     TFLAAYSLYKWDRKELDYKKYVGIILGILGGLISLLILGVAVIGLNVHRLAPYVEDTF
                     ARGNMEADVQWSGFEGAIGLLMVVALIMGIRYLSQRRYNRAAAALFVGTAVVVFFASA
                     IIVPKVERYSQGADIDFFIARQGEDCYVHTLGFKSYGDLFYTQKEKPTNLNSYDQNWL
                     LTGPID"
     gene            complement(1559..2308)
                     /locus_tag="VKQ52_17830"
     CDS             complement(1559..2308)
                     /locus_tag="VKQ52_17830"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ89123.1"
                     /translation="MIEGKKLVIVLPAYNAELTLRKTYAEIPFDIVDEVVLVDDNSSD
                     RTVAVGRELGIRHIIKHDHNRGYGGNQKSCYNKALELGGDIVIMLHPDYQYTPKLIPS
                     MAYLIANNVYQVVLGARTLGKGALRGGMPLYKYIFNRGLTFFENVMLRQKLAEYHTGY
                     RAFSREILQRIDYEINSEDFVFDNEMLSQIIYLGYEIAEITCPTKYFEEASSINFRRS
                     VTYGLGVLRVSIVHRFCKWGLMKREMYKPRN"
     gene            complement(2405..3043)
                     /locus_tag="VKQ52_17835"
     CDS             complement(2405..3043)
                     /locus_tag="VKQ52_17835"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLZ89124.1"
                     /translation="MKTLKAIITGNRLFFALWGLFFIVGLLFLLSVGKAASFVDLNPY
                     HQTVLDKVFMWITFMGDGLFSVIVVVLFLVRRRWSRATQVLAAFLLSALVAQIFKNAF
                     SMPRPKQFFAPGSYPYFIDGVTHLGFASFPSGHTTSIFALATILALFTKDQRAKIACL
                     LGAVVVGYSRIYLGQHFLGDVLMGSCIGTVVAVLVHWLFVVKLQSLPVFARG"
BASE COUNT          824 a          858 c          872 g          602 t
ORIGIN      
        1 tatcgatcgg ccccgtgagc agccagttct ggtcgtagga gttgagattg gtgggtttct
       61 ccttttgcgt ataaaagaga tcgccatagc ttttaaagcc gagggtgtgg acatagcagt
      121 cctctccctg gcgggcgatg aagaaatcga tgtccgcgcc ctggctgtag cgttccacct
      181 tcggaacgat gatcgccgag gcaaagaaga cgacgacggc ggtccccaca aaaagggcgg
      241 ccgcggcccg gttgtaccgc cgctgcgaca gatagcggat ccccatgatc agcgccacca
      301 ccatcagcag gccgatagcg ccttcgaacc ccgaccactg cacatcggct tccatattcc
      361 cgcgggcgaa agtgtcctcc acataggggg ccaggcggtg gacattcagc ccgatgacgg
      421 cgacgcccag gatcagcagg gagatcagcc cgccgagaat gccgaggatg ataccgacat
      481 attttttgta gtccagctct ttccggtccc acttgtagag ggagtaggca gccaggaagg
      541 taacggggaa ccaggccatg gaggaatagt ggatgatccg cgactgcacg atcgtaaaca
      601 acaccgtcac cacccaaaac aggatctgca tccatgtctt gaaatcccgg tcccaacggc
      661 cgttatactt ggcccggaag aaggagggaa tggccagtat cgaggcgggg aagcagccca
      721 ccagcaacac gatatagtga tagccaaaga acccggcctg atcggcatcg tgcgtggtaa
      781 agagccgcca ctggtatttg aggaattcat tgatgaacca ggggccccgc ttgatcgtct
      841 catagccgta ccaggtgaag gtaaccagga aggcgaccag gaggaagagc aaggcatgcc
      901 accagctgaa aaagaagcgg aaccggttat agagaaaata gacccccaac gccagcataa
      961 agaccatcag cgccacctgg cccttggtca ggacggccag tcccataaag atacccgacc
     1021 ataccacata atagaactgg ctcttttgtg cgcccggttt atcaaagcca ttccgtttcc
     1081 aatggtacag gatgaaatta tacagcgaga gaaaagtgaa aagattgaac caggggtcga
     1141 tgatccccga cttgaaatac atgttgggaa agagcgatcc gccataggcc agggcccaga
     1201 gaaggccgaa tttctggtca tagatccggc tgccgcagat aaagacgacg atcagggtga
     1261 cgatcccgca aagggcattc gggaagcggg cggcgaattc gttgacgcca aaaagatgca
     1321 tggccgtgct ttgcatccag aagaacatcg gcggtttctc ccagaagggc ttgaagtcga
     1381 catacacccg ggtataatca cgcagcttca tcatctcccg ggagcactcg gcgaaattga
     1441 tctcatccca gtcgaagagg tggacgcggc ccaggaaggg aaggaaaagc agggccgcga
     1501 acacggcgat cagcaggata gtggtggatt tattgaagcg catgcgaatt cggtggtttt
     1561 agtttcgcgg tttatacatt tctcttttca tcagccccca cttgcagaac ctgtgaacga
     1621 tggagacccg taataccccg agcccatagg tgacgctgcg gcggaaattg atactcgacg
     1681 cttcctcgaa atattttgtg ggacaggtga tctccgcgat ctcatagccc aggtaaatga
     1741 tctgggagag catctcgtta tcgaagacaa aatcttccga attgatctcg tagtcgatcc
     1801 gctggaggat ctcgcgggag aaagcccggt acccggtgtg gtattcggcc agcttttgcc
     1861 ggagcatgac attttcgaag aacgtgagcc cgcggttgaa gatatactta tagaggggca
     1921 tgccgccgcg aagcgcgcct tttccgagcg tccgggcgcc cagcaccacc tggtacacgt
     1981 tattggcgat gaggtatgcc atcgaaggaa tgagcttggg cgtatactgg tagtcgggat
     2041 ggagcatgat cacgatgtcg ccacccagct ccagcgcctt gttatagcaa ctcttctggt
     2101 tgccgccata gccccggtta tgatcgtgtt tgatgatatg ccggatacct aattcccggc
     2161 ccacggctac ggtccggtcg ctgctgttat catccaccag aaccacttcg tccacgatat
     2221 caaaagggat ctccgcatat gtcttccgga gggtcagttc cgcattgtac gcggggagta
     2281 cgatcaccag ttttttgcct tcgatcatgt aaagtcttga atagtaatat tcctatattc
     2341 ccattatggg aatgaagggt aaaaaaatcg cggtaagata acgtgttttt ggcttaaaac
     2401 cctgttatcc ccgcgcaaaa acgggcagcg actgcaattt gacgacaaag agccaatgca
     2461 caagcaccgc cacgaccgtt ccgatacagg agcccatcag cacatcgcca agaaaatgct
     2521 gccccaggta aatgcgggaa tagccgacca ccacggcccc aagcaggcag gcgatcttcg
     2581 cccgctggtc ctttgtaaac agcgccagta tcgtagccag cgcaaaaata gaggtcgtat
     2641 gtcccgaggg aaagctcgcg aagcccaggt gagtcacccc atcgataaaa taaggatagg
     2701 agccgggtgc gaagaactgt ttaggccggg gcatggagaa ggcgttcttg aagatctggg
     2761 ccaccagcgc cgagagcaga aaggcggcca gcacctgcgt tgcccgcgac caccggcgtc
     2821 gcaccaggaa caggacaacc acgatcaccg aaaagagccc atctcccata aaggtgatcc
     2881 acataaagac tttatccagc accgtctgat gatagggatt caggtcgaca aaggaggccg
     2941 ctttcccgac agagagcagg aacaacaggc caacgataaa aaagagcccc cacagggcaa
     3001 aaaaaaggcg gttgccggtg atgatcgcct taagcgtctt catccggcga aattacaata
     3061 aagatttgaa gctcactccc ccatgctcca atgtatccgg acgccggcac acgtcccgcc
     3121 ccgggccgat aggtcgctca cagccccgcc accaac
//
LOCUS       DATGFG010000945         4354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1174430, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000945 DATGFG010000000
VERSION     DATGFG010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174430"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="VKQ52_17840"
     CDS             <1..1369
                     /locus_tag="VKQ52_17840"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLZ89125.1"
                     /translation="QGGTFTQGATQEDVMRDWNNVPRRVTVNSFYIDQTEVANIHYRE
                     YLYWVNLVFDADEYKAVREAALPDTLCWRSELAYNEPMVEYYFRHPSYNFYPVVGVTW
                     RQAHDFCLWRSDRVNEKLLMDRGYINQNAVKNLQGLGQENFNTKAYLMGEFQATPDKR
                     ARTKSNPLKNPNGTPRTQVTFEDGILLPNYRLPTEAEWEYAALGYINQNPMPAKKDGQ
                     HGEELIANKQVYSWSNNVNGLRDTRHGSWQGTFMANFKRGAGDNMGVAGGLNDNAVYT
                     APVKSFFPNGFGLYNMSGNVSEWVGDVYRPMTGQDADDVSPYRGNKFQTVDLGKEGKA
                     ERDSLGRIKMRNVTDSESQARRNYQKGDVINYLDGDSTISGIAYGYGKTTLISNKSRV
                     IKGGSWNDRAYWLSPGTRRFLEEDQANSTVGFRCAMDRVGSPAGNGRQTGVNYKQRRQ
                     NTRKQ"
     gene            1450..2136
                     /locus_tag="VKQ52_17845"
     CDS             1450..2136
                     /locus_tag="VKQ52_17845"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HLZ89126.1"
                     /translation="MSKTAKPIQVILIVLLIIIADQTLKIWVKTHMPLSHSADAFSTR
                     VSPYDNGIHVLGSKAQIYFVENEGMAMGMSFGGSWGKMALTLFRLLAVIFGVYYIGSL
                     IKKKYHPGLIASAALIFAGALGNLIDSLFYGLLFEQSSYDHVARMFTGHGYAGFLHGE
                     VVDMLYFPLLKTQYPSWFPFMGGKEFEFFADIFNIADASITVGVLSILVFQKRFFVKA
                     NPAVLNHSAG"
     gene            complement(2117..>4354)
                     /locus_tag="VKQ52_17850"
     CDS             complement(2117..>4354)
                     /locus_tag="VKQ52_17850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ89127.1"
                     /translation="HWHYRGKDETGVAQEPPMAQALRNDLPQIAATGRLMPYALFEGA
                     GSNEVMPQGKTQDSYEEGFAYMDQSLLDMFQLPMVYGDRAHALAEPNTILISKRKADK
                     YFPGVDPVGKLLYLNDDKEHPHKIGGVMADLPANCHLHYDFFLTLTGKELWKDEQATW
                     MANNYDTYVLLKPGVDPVQMRAKMDRVLQTYWRPAMVRSGVPNVQELLDAEHLDLQPV
                     QNIHLDYTVDDGQSHGDTRFIWLFGAVAIFILALACINFINLSTARSANRAKEVGLRK
                     VVGSLRSGLIRQFLLESLVLSYFAFLLALGLAVVLLPLFNRLAATSLQIPWTQWWLLP
                     VLALGATLIGVLAGLYPSFYLSRFRPVEVLKGQLSRGSRHSGLRSVLVVFQFTTSVIL
                     IIATLVIYHQMQYIMNKKMGFDKDQVMLIQGTGTLGKQIKPFKNELLKLQGIKTASIS
                     DFLPISGGKRNQNQTFIAGREKIDPSVGAQTWWVDPDYVTTFGMKLVAGRMFSYDIAS
                     DSSAVVVNETLVRKLGLKDPIGKEIYTWYKQRIIGVVADFNFESVKQEIGPVVLHRGD
                     GATVIAVKTNGADMASLIKQVSAVWKSFLPQQEIRYTFLDESFARMYADVKRMGDIFT
                     SFALLAIVIACLGLFGLSAFMAEQRAKELGIRKVLGATVGQLAGLLSKDFLRLVLVSI
                     FIASPIAWWGMHKWLEDFVYRDDIGLWVFAVAGAVVILIALLTVSFQSVKAALTNPVT
                     NLRND"
BASE COUNT         1012 a         1300 c         1141 g          901 t
ORIGIN      
        1 ccagggtggc acattcacac agggcgccac ccaagaagat gtgatgagag actggaataa
       61 cgtacctaga cgcgtgactg tcaactcttt ctacatcgac cagacagaag ttgccaacat
      121 ccactaccgc gaatatctct actgggtcaa cctcgtcttt gacgccgatg aatacaaggc
      181 cgttcgtgaa gccgccctgc ccgacaccct gtgctggcgc agcgaactgg cgtataacga
      241 gccgatggta gaatactact tccgccatcc ctcttataac ttctatccgg tggttggcgt
      301 tacctggaga caggcccacg acttctgcct gtggcgcagc gatcgggtga atgaaaagct
      361 ccttatggat cgcggctata tcaaccaaaa cgccgttaag aaccttcagg gtctcggcca
      421 ggaaaacttc aacaccaagg cttacctgat gggtgagttc caggctactc ccgacaaacg
      481 tgcaaggacc aagagcaatc cccttaagaa ccccaacggt actcctcgta cccaggttac
      541 gttcgaagac gggatcctcc tccctaacta tcgcctcccg acagaagccg agtgggaata
      601 tgccgctctt ggctatatca accagaaccc gatgcccgcc aagaaagacg ggcagcatgg
      661 ggaagagctg atcgccaaca agcaggtcta ctcctggagc aataacgtca acggcctccg
      721 cgacacccgt cacggcagct ggcagggtac cttcatggcc aacttcaaac gtggcgccgg
      781 tgataatatg ggtgtggctg gtggtctgaa cgacaacgcc gtatacactg ctcccgtgaa
      841 atctttcttc cccaacggtt tcggtctgta taacatgtcc ggtaacgtga gcgagtgggt
      901 aggtgacgtc tatcgcccga tgacgggcca ggacgccgac gacgtttctc cctatcgtgg
      961 taacaaattc cagaccgtcg accttggaaa ggaaggaaaa gccgaacgcg acagcctggg
     1021 ccgtatcaag atgcgcaatg tcaccgactc cgaaagccag gctcgccgca actaccagaa
     1081 aggcgatgtt atcaactatc ttgacggtga ctccaccatc tccggaatcg cttatggtta
     1141 tggcaagact acccttatca gcaacaaatc ccgcgtgatc aagggtggta gctggaacga
     1201 tcgcgcctat tggctcagcc ccggtacccg ccgcttcctc gaagaagacc aggctaacag
     1261 tacggttggt ttccgttgcg ccatggaccg cgtaggcagc ccggcaggta atggccgcca
     1321 aaccggtgtc aactacaaac aaagacgcca gaatacccgc aagcaataaa aagattataa
     1381 ccagcgaaaa aagggtggca ccagcaacgg tggcaccctt tttctttaca tttgttcata
     1441 ttataatata tgtccaaaac cgcgaagcca atacaggtga tcctgatcgt gctgctcatc
     1501 atcatcgccg accagacact caagatctgg gtcaagacac atatgcccct tagccactcc
     1561 gccgacgctt tttctacccg cgtatccccc tacgataacg ggatccacgt gttgggcagc
     1621 aaagcacaga tctatttcgt cgagaacgag gggatggcca tggggatgag ctttggcggc
     1681 agttggggca agatggccct gaccctgttc cggctcctgg cggtgatctt cggcgtgtat
     1741 tatatcggca gccttatcaa aaagaaatac caccccgggc tcatcgcatc cgcggccctg
     1801 atcttcgccg gcgcgctcgg caatcttatc gatagcctgt tctatggact gctattcgag
     1861 cagagcagct atgatcacgt tgccaggatg ttcaccggtc acggctatgc cggcttcctc
     1921 catggagaag tagtggacat gctgtatttc cccctgctaa agacacaata cccgtcctgg
     1981 ttccccttca tgggcgggaa agagttcgaa ttcttcgccg acatcttcaa tatcgccgat
     2041 gcctccatca ccgtcggcgt cctcagcata ttagtcttcc agaaacggtt cttcgtaaag
     2101 gccaaccctg ctgtgctcaa tcattccgca ggttagtcac cgggttggtg agcgccgctt
     2161 tcaccgactg gaagctcacc gtcaaaaggg cgatgaggat gaccactgct cccgccacgg
     2221 caaagaccca taacccgata tcgtcgcgat agacaaaatc ctccaaccat ttgtgcattc
     2281 cccaccaggc gatcggcgag gcgataaaga tggagacaag caccagccgc aggaaatcct
     2341 tcgacaacaa tcccgccagc tggcccaccg tcgcacccaa taccttacgg atacccagct
     2401 cctttgcccg ctgctcggcc ataaaggcgg agagcccaaa gagccccaga caggcgatga
     2461 cgatggccag caaagcaaag ctcgtaaaga tgtcccccat gcgtttcaca tcggcgtaca
     2521 tacgcgcaaa actctcgtcg aggaaggtat agcgtatctc ctgctgcggc aggaagctct
     2581 tccacaccgc actcacctgt ttgatcaggg aggccatatc cgccccattt gtcttcacgg
     2641 cgatgaccgt agccccgtca ccgcggtgca ggacgacagg gccgatctcc tgctttaccg
     2701 attcgaaatt gaaatcggcc acaactccga tgatgcgttg tttataccag gtgtagatct
     2761 ctttcccgat gggatccttc aatcccagct tcctgaccag ggtctcgttg accaccacgg
     2821 ccgaggaatc ggaagcgata tcgtaggaga acatccgtcc cgcgaccagt ttcatcccaa
     2881 aagtggtgac ataatcgggg tccacccacc aggtctgggc gccgacggat ggatcgatct
     2941 tttcccggcc cgcgataaag gtttgattct gattccgttt gccgccgctt atcggcagga
     3001 aatcgcttat cgaggcggtt tttatgccct gtagtttcag cagctcgttc ttgaacggct
     3061 ttatctgctt ccccagggtc ccggtaccct ggatcagcat cacctggtcc ttatcaaagc
     3121 ccatcttctt attcatgatg tattgcatct ggtgatagat caccagcgtg gcgatgatga
     3181 ggatcaccga tgtcgtgaac tggaagacca cgagcacgct ccgcagcccg gaatggcggc
     3241 tgccacggct caattgtccc ttcagcacct ccacgggacg gaaacgtgac aggtagaagc
     3301 ttgggtaaag ccccgccagc acgccgatga gcgtggcacc cagggccaac accggcagca
     3361 gccaccactg ggtccagggg atctgcaggg aagtggccgc cagccggttg aacaggggta
     3421 acaggacgac cgccaggccc agcgccagca ggaaggcaaa atagctcagc acgagtgact
     3481 cgagcaggaa ctgccggatc agcccactgc gcagggaacc gacgaccttc cgcaggccta
     3541 cctcctttgc gcggttagcg ctgcgggccg tcgacaaatt gataaaattg atacaggcca
     3601 gcgccaggat aaaaatggcg acggcgccga acagccagat aaagcgggta tcgccgtggg
     3661 actggccatc atcgacggta tagtcgaggt ggatattttg caccggctgc agatccaaat
     3721 gttcggcatc cagcaattcc tgcacgtttg gcaccccact ccggaccatc gccgggcgcc
     3781 agtaggtctg tagcacccgg tccattttcg ccctcatctg caccgggtcg acaccgggtt
     3841 tcagcaggac atatgtgtcg tagttattgg ccatccaggt ggcttgctcg tctttccaca
     3901 gctccttgcc cgtcagcgtc aggaagaaat catagtgaag gtggcagttg gccggcaggt
     3961 ccgccatgac cccaccgatc ttatgaggat gctctttgtc gtcgttcaga taaagcagct
     4021 taccaacggg gtcgacaccg ggaaaatatt tatccgcctt gcgtttggag atgaggatgg
     4081 tattgggttc ggcaagcgca tgtgcgcggt cgccatagac catcggaagt tggaacatgt
     4141 ccagcagcga ctggtccata taggcaaagc cctcttcata gctgtcctgg gtcttgccct
     4201 gcggcatcac ctcattgctg cccgcgcctt cgaacagcgc atagggcatg agccggcccg
     4261 tcgccgctat ctgcggcagg tcgttcctga gggcctgcgc catgggtggc tcctgtgcaa
     4321 cgcccgtttc gtccttaccc cgatagtgcc agtg
//
LOCUS       DATGFG010000946         6054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1649948, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000946 DATGFG010000000
VERSION     DATGFG010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649948"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /gene="prfA"
                     /locus_tag="VKQ52_17855"
     CDS             <1..1020
                     /gene="prfA"
                     /locus_tag="VKQ52_17855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HLZ89128.1"
                     /translation="GVALSNPEVVSDNRRFSAMSKEYRSIEKIVRAHDEYKNLLDTID
                     FNKEALNGDDAELRELAKAEAPGLEERKTQLEAHIRQLLIPKDPQDEKNAILEIRAGT
                     GGDEASLFAGNLLRMYIKYCERRGWKTAILSESEGTVGGYKEVQVEVVGDDVYGTLKF
                     ESGVHRVQRVPDTETSGRVHTSAATVAVMPEAEEVDFELRESDVKMETARSGGAGGQN
                     VNKVETKVFLTHLPTGVTVVCQTERSQLGNREKAMQMLRTKLYEEQVRKQEEEIARHR
                     KSLVSTGDRSAKIRTYNFPQGRVTDHRIGLTVYNLDDVLNGEIQEFLDALQFAENAEK
                     MARQN"
     gene            complement(1602..2012)
                     /locus_tag="VKQ52_17860"
     CDS             complement(1602..2012)
                     /locus_tag="VKQ52_17860"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HLZ89129.1"
                     /translation="MLGFIKKYFSALYVPVIWTLLVALAMCIPGRYIPDESGFALPNF
                     DKFVHLVLFGGFVFLWSLYLEKRGIPFKKLVFGFFCFYMLSNGFGIAMELVQKYWIPG
                     RDYDPGDIIADMFGAGFGYGFSNLLLLPGKQKND"
     gene            complement(2114..2884)
                     /locus_tag="VKQ52_17865"
     CDS             complement(2114..2884)
                     /locus_tag="VKQ52_17865"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HLZ89130.1"
                     /translation="MGLASESERAEFEQYCAQYPELVAARRKFEEKLEAYASENAVPP
                     PPEVKVKVLEAVGMESRAASSIPPKIINMENAKTPVRSSDMLTFIAAASVVLLIGMAY
                     LYFQTRQENKDLTGANDRLRESLDTTRNILNRIVQEQKVVGDPNTTVVNMVGTTVAPK
                     SSANVYWDSASSSVYLVVKNMPKLPSDQQYQLWALIDGKQAPKDLGVFDATDDKVILK
                     MKNTQKAQAFAITIEKKGGSPSPTLQKMQSLGKMMQGQ"
     gene            complement(2929..3477)
                     /locus_tag="VKQ52_17870"
     CDS             complement(2929..3477)
                     /locus_tag="VKQ52_17870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ89131.1"
                     /translation="MGIPTTYTEQELVLALKERNNQAFGFLYDNYAGALYSIIRQIIT
                     DNSELASDVLQEVFINIWRKIETYDQTKGRLFTWMLNIARNASIDTLRSKSYQNSQKN
                     QELPDNVYKGVASQTTQQNVDNIGLKKVLETLRPEHRVLVELAYFKGFTHEEIADMLS
                     IPLGTVKTRIRNALLQLREYLK"
     gene            complement(3519..3899)
                     /gene="gcvH"
                     /locus_tag="VKQ52_17875"
     CDS             complement(3519..3899)
                     /gene="gcvH"
                     /locus_tag="VKQ52_17875"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HLZ89132.1"
                     /translation="MNFPDNLRYTKDHEWIRLEKDNVAIIGITEFAQKELGDIVYVEV
                     ETVGKALAAETVFGTVEAVKTVSDLYLPVAGTILELNPELGSAPDLVNSDPYGKGWMI
                     RMTVKDPADLDKLMDATAYANIVH"
     gene            complement(3994..4707)
                     /locus_tag="VKQ52_17880"
     CDS             complement(3994..4707)
                     /locus_tag="VKQ52_17880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLZ89133.1"
                     /translation="MTIRRLLPAAVLLLLVSACNPKLSNGLRKNDLHRDVAVITTKGT
                     LVIRLSDSTPLHRDNFLLLVKKGYYDSLLFHRVIRNFMIQSGDPDSREGHPGKPLSQG
                     GSGGPGYTIPAEFRPGLFHHRGALGAARTSDDVNPTRASSGSQFYIVQGKRFTDGGLD
                     SLEVTRLKGRKIPPAQREVYREIGGAPHLDQAYTVFGEVIRGMDVVDSIAAVATSGPP
                     LDRPKTEVRILKMRLIRRK"
     gene            complement(4704..5072)
                     /locus_tag="VKQ52_17885"
     CDS             complement(4704..5072)
                     /locus_tag="VKQ52_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89134.1"
                     /translation="MQQLQDDLQARWWQLEARLLERFGKKPDMETLLFLIGVQEFGDI
                     RKKFTKEEKQDLMHIAVCTLFAQSGYYRLDRVDEQGWPHFIQLKALPVMEVVQQENFM
                     KDHILLYFDNAEIGEEPDNL"
     gene            complement(5163..5495)
                     /locus_tag="VKQ52_17890"
     CDS             complement(5163..5495)
                     /locus_tag="VKQ52_17890"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HLZ89135.1"
                     /translation="MIDPTMITTSTALEGHRLVRNLGVVRGITVRSRSVLGNLAGGLQ
                     TLFGGQISIYVELCEKTREEAFQYMMQHAAERGANAIINMRYDANEVMNGVTEVLAYG
                     TAVVVERV"
     gene            5555..>6054
                     /locus_tag="VKQ52_17895"
     CDS             5555..>6054
                     /locus_tag="VKQ52_17895"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89136.1"
                     /translation="MLQVAFIRQNVEYVKERLGVRNFTETGLVDTIVALDDQRRKLQL
                     ETDTTQSKINAHSKEIGALMAKGQKQEAEQKKQEVAALKTSLNPIADQLAAVEEELNN
                     TLVRLPNLPAAAVPRGRTPEDNITIRESGAKPTLPAGAVPHWDLAKKYNLIDFELGNK
                     ITGSGF"
BASE COUNT         1560 a         1745 c         1433 g         1316 t
ORIGIN      
        1 ggagtagccc tgagcaaccc ggaagtcgtc agcgacaacc ggagattcag cgccatgagc
       61 aaggaatacc gtagtatcga gaagatcgtg agagcccacg atgaatataa gaacctgctg
      121 gacaccatcg atttcaataa agaagccctc aacggcgacg acgcggaatt gcgcgaactg
      181 gccaaggccg aagccccggg gttggaagaa cgtaaaacgc aactggaagc ccatatccgg
      241 caactgctga tacccaaaga cccgcaggat gagaagaacg ccatcctcga gatccgggcg
      301 ggaacaggcg gcgacgaggc cagcctcttc gccggcaatc tgctgcggat gtatatcaaa
      361 tactgcgaac gccgcggctg gaagaccgcc atcctgagcg aaagcgaagg caccgtaggc
      421 ggctataagg aagtacaggt ggaagtggtc ggcgacgatg tctatggaac actaaaattc
      481 gagagcggcg tccaccgcgt ccaacgggtc cccgacaccg aaacaagcgg ccgcgtacat
      541 accagcgccg ccaccgtggc cgtcatgccc gaagccgaag aagtcgattt cgaattgagg
      601 gaaagcgacg tcaagatgga aaccgcccga agcgggggcg ccggcggcca gaacgtcaac
      661 aaagtggaaa caaaagtctt ccttacccat ctccccaccg gggtaaccgt cgtctgccag
      721 accgaacgca gccaattggg caaccgcgaa aaagccatgc agatgctgcg gacaaaactc
      781 tatgaagaac aggttcgcaa acaggaggaa gagatcgccc gccaccgcaa aagcctcgtc
      841 agcaccggcg accgcagcgc caagatccgg acctacaact tcccgcaggg ccgggtcacc
      901 gaccatcgca tcggcctcac cgtctataac ctggacgacg tcctcaatgg cgagatccag
      961 gaattcctcg acgccctgca gtttgccgag aacgccgaaa aaatggcccg acagaactag
     1021 accacaggca taccccatat accacaaact gggtatgatc cgccgccagc ctgatttcca
     1081 cgcgattaca cggacggccc cggcggtaaa aaggtcgccc tgacattaaa acagaattaa
     1141 aagcccccgt aaaagcaaca gaaagcggct cctttcacgt tattttaacg gcagatgatt
     1201 aaactttgcc gctacaacac tgtctttata taggcagaca gccgttagtt ctttaaaaaa
     1261 tcccgctaaa acttgctata gagcaggaat ttgtataact taataagggg taatgctaaa
     1321 taaatgttat tactcccaat tagggaaacg aaaaaacgat ttccatcgta attttgggta
     1381 gtgaagatcg aaagcaggaa agcgggtgat gatcgaagtt ctttaacact gacgaaacac
     1441 aacattggca cctgttttgc gttatactag gtagttaatt cagtcttttc tatttgactc
     1501 acataaagca gtcaattttc tcataagcag ttagttttgg ttacgacccc tgtttctaca
     1561 ggggtttatt tttttataac cggtcaccct aacagtaacg cctaatcgtt cttctgcttc
     1621 ccgggcaaca gcaaaagatt actgaaccca tacccgaatc ccgccccgaa catatcggca
     1681 atgatatctc cggggtcata gtcccgcccc gggatccagt atttctgaac cagttccatc
     1741 gcgataccga acccattgga aagcatataa aaacagaaga aaccaaacac cagtttttta
     1801 aacgggatcc cgcgcttctc caggtaaaga ctccagagga acacaaatcc gccaaacagc
     1861 acaagatgta cgaatttgtc gaaattgggg agggcaaatc cactctcatc cggaatatac
     1921 ctgcccggga tacacatcgc aagggcgacc agcagggtcc aaatgacggg aacatacagc
     1981 gcggaaaagt atttctttat aaaaccaagc ataatacggg aataggccgg aaattaaaaa
     2041 aagtccccga gagtgagact ttttttctat agctgtagaa tttctttcct accggcttcc
     2101 ccggctcttc ggcctattga ccctgcatca tcttaccaag cgactgcatt ttctgcaggg
     2161 taggggaagg gctgccgccc tttttctcga tcgtgatggc aaaggcctgc gccttctggg
     2221 tattcttcat cttcaggatg accttgtcat ccgtggcgtc gaatactccc agatccttgg
     2281 gcgcctgctt accgtcgatc agcgcccaca actggtactg ctggtccgaa ggcagcttcg
     2341 gcatgttctt gaccaccagg taaacgctcg acgaagccga atcccaatag acattggccg
     2401 aagacttcgg ggctaccgtc gtcccgacca tattgaccac cgtggtgttc gggtcgccta
     2461 cgaccttctg ttcctgtacg atccggttca ggatattcct ggtggtgtcc agggactccc
     2521 ggagacggtc attggcgccg gtaagatcct tattctcctg ccgggtctgg aagtagagat
     2581 aggccatacc gataagtaat acgacagagg cggcagctat gaaggtaagc atgtcggacg
     2641 agcggactgg agtctttgca ttttccatgt tgatgatttt aggtggaatg gaagaagccg
     2701 cccgtgattc catgccgaca gcttccaaaa ctttcacctt aacctctgga ggggggggca
     2761 ccgcattctc ggatgcatat gcctcaagct tttcttcaaa cttccttcgt gctgctacca
     2821 actcaggata ctgagcacag tactgctcaa attcagctct ttccgactca ctcgccagac
     2881 ccatcacata gctctcgatg atgccgcttt ctatgtattc ttgtacgttc actttagata
     2941 ttctcttaat tgtagtaacg cattcctgat ccgggtcttc acggtaccaa ggggaataga
     3001 tagcatatcc gctatctcct cgtgcgtaaa gcccttgaag taagccagtt ctaccaaaac
     3061 ccggtgctct ggccgcaacg tctccagcac cttcttcagg ccgatattat cgacgttctg
     3121 ctgcgtggtc tggctggcca cgcccttata tacgttgtcc ggtagctcct ggtttttctg
     3181 ggagttctga taacttttcg atctgagggt atcaatggaa gcattcctgg caatattcaa
     3241 catccaggta aagagacgtc ccttggtctg gtcgtaggtt tctatcttcc gccagatatt
     3301 gataaacacc tcctgcaata catcgctcgc taactcggaa ttatcggtaa tgatctgtct
     3361 gataatgcta tagagcgccc cagcataatt gtcatacaga aaaccgaagg cctggttgtt
     3421 cctttctttc agcgccagca caagctcctg ctctgtatag gtagtaggta ttcccaaggt
     3481 ttatgtaaat agatacgcta aagtaaacaa taaaaagatc agtgcacgat gttggcatag
     3541 gccgtggcat ccatcagctt atccaggtcg gcaggatctt tcaccgtcat cctgatcatc
     3601 catccttttc catacggatc gctattgaca agatccggcg ccgaacccaa ttcgggattc
     3661 aattccagga tcgtacccgc caccggcaga taaaggtcag acaccgtctt taccgcctcg
     3721 acagtcccaa agacggtctc ggcggcaagc gctttcccta ccgtttccac ctccacataa
     3781 acgatatccc ccaattcctt ttgtgcaaac tccgtaatgc cgatgatggc gacattatcc
     3841 ttttccagcc ggatccattc atggtctttc gtataacgta aattatcagg gaagttcatg
     3901 gtagctaaat ttttatgctg taagaattat aaaattatta tggtgcaaga ttacaaaaag
     3961 cccccgaaat aagcggagaa agaggataaa actttatttg cggcggatga gcctcatttt
     4021 caatatcctt acctcggttt taggccggtc cagcggcgga ccgctggtgg caacggccgc
     4081 aatggaatcc accacatcca tcccccggat cacctccccg aataccgtat aagcctgatc
     4141 gagatgtggc gcccccccga tctccctgta cacctcccgc tgcgccggcg ggatcttcct
     4201 gcccttcaac cgcgtgacct cgagggaatc cagcccgcca tcggtgaacc gcttcccctg
     4261 gacgatatag aactggctgc cgctactggc cctggtggga ttgacatcgt cactcgtcct
     4321 cgccgcgcca agcgccccac ggtgatggaa cagacccggc cggaactctg ccggtatggt
     4381 atacccgggg cccccgctgc caccctggct cagcggcttg ccgggatgac cttccctgct
     4441 gtccggatcc ccggactgga tcataaaatt ccggatcacc cggtgaaaga gcaggctgtc
     4501 ataataccct ttcttcacca gcaacaggaa attatcccgg tgaaggggag tcgaatcgga
     4561 cagccggatc accagggtac ccttcgtggt aatgacggct acatcccggt gaaggtcgtt
     4621 cttccggagg ccgttggata atttgggatt acaggcagag accagcagca ggaggaccgc
     4681 cgccggcaac agtcttcgta tcgtcataag ttgtcaggtt cttcacctat ctccgcattg
     4741 tcgaaataca acaggatatg atccttcatg aaattctcct gctgcaccac ctccatcacc
     4801 gggagagcct tcagttggat aaaatgcggc cagccctgct cgtccacccg gtcgagacga
     4861 taatatccgc tctgcgcgaa aagcgtgcag accgcaatat gcatgaggtc ctgtttttct
     4921 tccttggtaa acttcttccg gatatctcca aattcctgca cgccgataag gaataaaagg
     4981 gtctccatat cgggcttctt gccaaaccgc tcgagcagcc gcgcctccag ctgccaccac
     5041 cgcgcctgca gatcgtcctg taactgttgc atggatgtaa agatacgaca cttccaatac
     5101 ttccggccaa caccgtcttc cccggccaac accgccgccc tgtaccatcc cggccactgc
     5161 ccctataccc tctccaccac taccgccgtc ccgtaggcaa ggacttccgt caccccattc
     5221 atcacctcgt tggcgtcata gcgcatattg atgatcgcat tcgccccacg ctcagcggca
     5281 tgctgcatca tgtactggaa agcttcctcc ctcgtcttct cacaaagctc cacataaata
     5341 gagatctgtc cgccaaaaag ggtttgcaat ccgccggcaa ggttgccgag tacgctgcgg
     5401 ctccggacag tgatgccacg tacgacaccc aggttgcgga caagccggtg tccctccaat
     5461 gcagtgctcg tggtgatcat ggtagggtct atcatataaa aggttttctc caatatacac
     5521 caatccggta ttacctttgc ccaaaaaaca gaaaatgtta caagtggcat ttatccgtca
     5581 gaacgttgag tatgtaaagg agagactggg agtaaggaat ttcacggaga ccgggctggt
     5641 cgacaccatc gtagccctcg acgatcagcg cagaaaattg caactcgaaa ccgacaccac
     5701 ccagtcgaag ataaatgccc attccaaaga gatcggcgcc ctcatggcaa agggacaaaa
     5761 acaggaggcg gaacaaaaaa aacaggaagt cgcggcgctg aaaacatcgc tcaaccccat
     5821 cgctgaccaa cttgccgccg tcgaggaaga actgaacaat accctcgtcc gcctgcccaa
     5881 cctccccgca gccgccgtcc cccgcggccg cacgcccgaa gacaacatca ccattcgcga
     5941 aagtggcgcc aaacccaccc tgccggccgg cgccgtcccg cactgggacc tcgcaaaaaa
     6001 atacaacctc atcgacttcg aactggggaa caagatcacg ggcagcggct tcca
//
LOCUS       DATGFG010000947         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_88844, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000947 DATGFG010000000
VERSION     DATGFG010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88844"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VKQ52_17900"
     CDS             <1..906
                     /locus_tag="VKQ52_17900"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLZ89137.1"
                     /translation="VVLVVLAIIFSCVVTVNQGYIYVVTMFGKYRRIFRPGFNLKLPF
                     LEAVYKKISIQNRSVELEFQAVTADQANVYFKSMLLYAVQNAEEETIKRVAFKFIADR
                     DLMQALTRTIEGSIRSFVATKRQAEILGLRKEIVEFVKGQIDHSLEEWGYHLMDLQIN
                     DITFDKAIIESMSKVVASNNLKAAAENEGQALLITKTKAAEAEGNAIKISAEAEKEAA
                     RLRGQGVALFRQEVAHGMSEAAEEMKRANLDTNVILFSMWTEAVKNFAELGKGNVIFL
                     DGSAQGMENTMKQIQALMMKPAGTK"
     gene            1101..1805
                     /locus_tag="VKQ52_17905"
     CDS             1101..1805
                     /locus_tag="VKQ52_17905"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HLZ89138.1"
                     /translation="MNLFFLQVILDSAKIKDSLARVAQMAAQQKESLYDILLKGGPLM
                     IPLAIILVVAIFVFFERYLAIKKAAKMDENFMSIIRDHIINGNIQAARSLTKNTNTAV
                     ARMIDKGIQRVGKPIEAIERSMENVGKLELYKMEKNLSVLSVVGYIAPLFGFLGTIAG
                     MFQLFYSIANTGEYSISNIAGGIYVKMISSASGLIIGIIGYLAYRFLNSQIDKATNRM
                     EVASADFIDILQEPTR"
     gene            1821..2225
                     /locus_tag="VKQ52_17910"
     CDS             1821..2225
                     /locus_tag="VKQ52_17910"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HLZ89139.1"
                     /translation="MNLRRRHKDDAEVHTGPLNDILFILLLFFLIVSTLANPNVVKLS
                     QPKSKSDTKAKQTVVVSIDANKQFYVGTTRVTIEELKAKLQPFLAKETDQPSIVINAD
                     KSVPVDDIVQVMRIARDLGARTVLAVDKNGVQ"
     gene            complement(2228..3112)
                     /gene="prmC"
                     /locus_tag="VKQ52_17915"
     CDS             complement(2228..3112)
                     /gene="prmC"
                     /locus_tag="VKQ52_17915"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HLZ89140.1"
                     /translation="MDWSYAQKELSGCLLPLYGDREASIIADWVMEALSGMRRLDRLM
                     VRATPLSDAELAGYERYKAQLLAHRPVQYVLGESWFAGMRFFVDERVLIPRPETEELV
                     EWVISEVAGAGAADAGPERRGGEGRLLDVGTGSGCIAVTLARRLAGVGVVACDISEGA
                     LAVARKNAAELGAEVGFFELDFLDRDAWERLPSLRWLVSNPPYIPVGERLAMARHVID
                     HEPASALFVPDGDALVFYRALGEFARERLGPGGSLFAEIHEDLGPAVCDLLTALGARE
                     VHLRKDLQGKDRMIKAGW"
     gene            3151..4191
                     /gene="ribD"
                     /locus_tag="VKQ52_17920"
     CDS             3151..4191
                     /gene="ribD"
                     /locus_tag="VKQ52_17920"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HLZ89141.1"
                     /translation="MTTPETYMHRCLELARLGAGHVAPNPMVGAVLVYEDRIIGEGYH
                     RLYGKAHAEVNCIDSVSEADSGFIDKSTIYVSLEPCAHHGKTPPCADLIIAKNIPRVV
                     IGCRDPFPQVDGKGIGKLKAAGIEVTLGVLEKECIDCNRRFFTFNTRHRPYIILKWAQ
                     SANGFIARAGERTPISNDYTNRLVHKWRSEEASILVGPRTALIDNPALTARLWTGPDP
                     VRLVIDKNLELPPDLRLFDKKTRTIVFNTIKHEETRDNLEYYQVAGDSSLIDQLLIAL
                     HHMKIQSVLVEGGARLLQSFIDADAWDEARVITNNELALPAGLPAPRLTNAHLRSHES
                     LLSDTIRYYHHD"
     gene            4184..4795
                     /locus_tag="VKQ52_17925"
     CDS             4184..4795
                     /locus_tag="VKQ52_17925"
                     /inference="COORDINATES: protein motif:HMM:NF013378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HLZ89142.1"
                     /translation="MTEQDFYYTIAKSGKAEFKDRGSKFIGYVYPVSSVEEFKERLND
                     TKKEHPKATHHCFAYRLGLDGNTHRVSDDGEPSGTAGRPILGQIDSKQLVNVLVVVVR
                     YFGGTLLGVPGLINAYKSAAALALQTTLQVQKPVEKEFVVQFDYTQVNEIMTLVKQYN
                     CRVVRQEMQLFCNMTLAIPRNRVPEVTYKLQELRNVELSPAIG"
     gene            4845..>5688
                     /locus_tag="VKQ52_17930"
     CDS             4845..>5688
                     /locus_tag="VKQ52_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ89143.1"
                     /translation="MKKPPVSLALALLFLAAGFIACQKYASSSNSSTSTNPFPAEVTV
                     TASVQGRVVDENDIPVEGATVTSGTASTSTDINGIFTFKNISLSSRFGFVKASKTGYF
                     TGSRSVATDGGESNFVSIQLIPRNETGNFAAASGGKIMVFSGDTVSFSPSSVVNAVTN
                     AAYTGTVHVFATYLNPTNDNLTKYMPGDLRGISTSGKEVALQTYGMMSVELQDDAGNK
                     LQLASGQQATITMAIPDLLQSTAPATIPLWYFNDSTGRWIQQGSATRQGNNYVGSVGH
                     FTWWN"
BASE COUNT         1376 a         1829 c         1408 g         1075 t
ORIGIN      
        1 gtggtcctcg tcgtcctggc cattattttc agctgtgtcg tcaccgtcaa tcagggatat
       61 atttacgtag ttaccatgtt tgggaagtac cggcgcatct tccggcccgg attcaacctg
      121 aaactgccct ttctcgaagc ggtgtacaag aagatctcca tccagaaccg gtcggtggaa
      181 ttggaattcc aggccgtcac cgccgatcag gccaacgtct attttaaaag catgctcctc
      241 tacgccgtac aaaatgccga agaggagacg atcaaaaggg tcgccttcaa gttcatcgcc
      301 gaccgcgatc tgatgcaggc cctcacccgt accatcgaag gcagcatccg ttccttcgtc
      361 gccaccaagc gccaggcgga gatcctcggt cttcggaagg agatcgtcga attcgtaaaa
      421 ggccagatcg accattcgct ggaagaatgg ggctaccacc tgatggacct ccagataaac
      481 gatatcacct tcgacaaggc catcatcgag tcgatgagca aggtcgtggc cagcaataac
      541 ctcaaggccg ccgcggagaa cgaaggccag gccctcctca tcacaaagac aaaggccgcg
      601 gaagccgagg gcaatgccat caagatctcc gccgaggcag agaaagaagc cgcccgtctc
      661 cgtggtcagg gtgtcgccct cttccggcag gaagtcgccc acgggatgag cgaggccgcc
      721 gaagagatga aacgggccaa cctggatacc aacgtcatcc tcttcagcat gtggaccgaa
      781 gcggtcaaga acttcgccga actggggaag ggaaacgtca tcttcctcga tggcagcgcc
      841 cagggcatgg agaacacgat gaagcagatc caggcgctga tgatgaagcc ggccggtacc
      901 aaatagctgc ctgtcgccgc tattcggcac aacagggcgg accacgggtc cgccggccga
      961 aggcccgata ttagccaaat tgtgccgaat ggctaatttt cgacggagcc attcggcaca
     1021 attttagcat ttcgttagaa aattcaggaa aatacaatgg cccacaggcc gttatattcg
     1081 caaatcttta catttaccac atgaatctat tcttcctgca ggtgatcctc gactcggcaa
     1141 agatcaaaga cagcctggcc agagtggcac agatggcagc ccagcaaaaa gaaagtttat
     1201 atgatatcct gctgaaaggc ggccccctca tgatccccct ggccatcatc ctggtggtag
     1261 ccatcttcgt tttttttgaa cgctacctgg ctatcaaaaa ggccgccaaa atggacgaaa
     1321 atttcatgag catcatccgc gaccatatca tcaacgggaa tatccaggcc gcccgcagcc
     1381 tgacaaagaa taccaacacc gccgtagccc ggatgatcga taaaggcatt cagcgcgtgg
     1441 gaaaacccat cgaagccatc gaaagaagca tggagaacgt ggggaaactg gaattgtata
     1501 aaatggagaa aaacctgagc gtcctctccg tggtcggcta tatcgcaccc cttttcggct
     1561 tcctcggcac catcgcgggg atgttccagt tattctacag tatcgccaac accggggaat
     1621 acagcatctc caacatcgcc ggcggcatct atgtaaagat gatctcctcg gcttcggggc
     1681 tgatcatcgg gatcatcggc tacctggcct accgtttcct gaattcccag atcgataagg
     1741 ccaccaaccg gatggaagtg gcaagcgccg atttcatcga tatcctgcag gagccgaccc
     1801 gctaacagaa aaccagcaaa atgaacctac gaagaagaca taaggacgat gcggaagtac
     1861 ataccgggcc gttgaacgac atcctgttca tcctgctgct gttcttcctc atcgtttcca
     1921 ccctcgccaa cccgaatgtg gtaaaactat cccagccaaa gtcaaaaagc gatacaaagg
     1981 ccaaacagac cgtcgtcgtc tccatcgatg cgaataaaca gttctatgtc ggcaccaccc
     2041 gcgtcacgat agaggagcta aaggcaaaac tgcaaccctt tctggcaaaa gagaccgacc
     2101 agccttcgat cgtcatcaac gcggataagt ccgtccccgt cgatgatatc gtccaggtca
     2161 tgcgcatagc ccgcgatctc ggcgcccgta ccgttcttgc ggtagacaag aacggcgtcc
     2221 agtaatatta ccatcccgcc tttatcatcc ggtcctttcc ctgtaagtct ttgcgcaagt
     2281 gcacctcccg ggcgcccagc gcggtcagca gatcgcacac ggccggaccc aggtcctcat
     2341 ggatctcggc aaaaaggcta ccccccggcc cgagccgctc ccgggcgaac tcgccaaggg
     2401 cccggtagaa gacaagcgcg tcgccatcgg ggacaaaaag cgccgaggcc ggctcatgat
     2461 cgataacgtg acgggccatc gcaagccttt cgcccacagg gatatacggc ggattactaa
     2521 ccagccagcg tagggacgga agccgctccc acgcatcccg atcaaggaaa tccagttcga
     2581 agaaccccac ttccgctccc agttcagccg catttttccg cgccaccgcc aacgctcctt
     2641 cgctgatatc gcaggccacc actcctacac ccgccagccg ccgtgccagc gtaacggcga
     2701 tacacccgct acccgttccc acatccagca gccggccctc accgccccgc ctctccggcc
     2761 cggcatcggc agctcccgct ccggcaactt cggagatcac ccactccacc agttcttcgg
     2821 tctccggccg ggggatcagg acccgctcat ccacaaagaa ccgcattccc gcgaaccagc
     2881 tctcgcccaa tacatattgc acgggacggt gcgccagcaa ctgcgcctta tatcgctcat
     2941 agccggccag ctccgcatcc gacaacggag tagcacgtac catcagccgg tcgagcctcc
     3001 gcatcccgct aagcgcctcc atcacccagt cggcaatgat cgaggcctcc cgatcgccat
     3061 acagcggtag caggcaaccg ctcaattctt tttgcgcata agaccaatcc atccggtaaa
     3121 gttaccccca attgggttaa atttgcgccc ttgaccactc ccgaaacata tatgcaccgc
     3181 tgcctcgaac tggctcgtct cggcgccggc catgtcgctc ccaatccgat ggtgggcgcc
     3241 gtgctggtat acgaagaccg catcatcggc gaggggtatc atcgactcta cggcaaggcg
     3301 catgctgaag tgaattgcat cgactccgta agcgaagcgg acagcgggtt tatcgataag
     3361 tccaccatct atgtttcact cgaaccctgt gcccatcatg gtaagacccc gccctgcgcc
     3421 gacctcatca tcgcaaagaa catcccccgc gtggtcatcg gctgccgcga cccctttccg
     3481 caggtcgatg ggaagggcat cgggaaacta aaagccgcag gcatcgaagt caccctcggg
     3541 gtcctggaaa aggaatgtat cgattgcaac cggcggttct tcaccttcaa cacccggcac
     3601 cggccctata tcatcctgaa atgggcacaa agcgccaacg ggttcatcgc ccgcgccggc
     3661 gaacgaaccc ccatttccaa tgactatacc aaccggctcg tccataaatg gcgttccgaa
     3721 gaggcctcca ttctcgtcgg cccccgcacc gcgctgatcg acaacccggc gctgacggcc
     3781 cggctctgga ccggccccga tcctgtccgg ctggtcatcg acaagaacct cgaattacct
     3841 ccggatctcc ggctcttcga taaaaagaca aggaccatcg tcttcaatac gatcaaacac
     3901 gaagagaccc gggataacct ggagtattac caggtcgccg gggatagcag cctcatcgat
     3961 cagctcctca tcgccctcca ccacatgaag atccaaagcg tattggtgga aggcggcgcc
     4021 cggctcctgc agtcctttat cgacgcggac gcctgggatg aagcaagggt catcaccaat
     4081 aacgaacttg ccctgcccgc cggcctgccg gccccccgtt tgacaaacgc gcacctccga
     4141 tcccatgaaa gcctattgtc ggacactatt cgttattacc atcatgactg agcaggattt
     4201 ctattacacc atcgccaaaa gcggaaaggc cgagttcaag gacaggggca gcaaattcat
     4261 cggctatgtc tacccggtat cctccgtgga ggagttcaag gaaaggctca acgacaccaa
     4321 aaaagagcac ccaaaggcca cccaccactg cttcgcctat cgcctcggcc tcgacggcaa
     4381 tactcaccgt gtgagcgatg acggagaacc ctcgggcacc gccggccggc ccatcctggg
     4441 acagatcgac agcaaacagc tggtcaatgt cctcgtggtc gtcgtccggt atttcggggg
     4501 gaccctgctc ggcgtgccgg gcctgatcaa cgcctataaa tccgccgccg ccctcgccct
     4561 gcaaacgacg ctgcaggtcc aaaaaccggt ggagaaggag ttcgtggttc agttcgacta
     4621 tacccaggtg aacgagatca tgacccttgt caaacaatac aattgccggg tcgtccgcca
     4681 ggaaatgcag cttttttgta atatgaccct cgccatcccc aggaacaggg tacccgaagt
     4741 cacttataag ctacaggaac tcaggaatgt agaactttct cccgccattg gataatttcg
     4801 ggctgcggtt atccgtttaa taggccaacc caatctgagc ccccgtgaaa aaaccacccg
     4861 tatcccttgc ccttgccctg ctgttcctgg ctgccggctt catcgcctgt cagaaatatg
     4921 cctcctcaag caattcctcc acctctacca acccattccc ggccgaagtc acggtaaccg
     4981 ccagcgttca gggccgcgtc gtcgatgaga atgacatccc ggtggaaggc gcgacagtca
     5041 ccagcggcac ggccagtacc tccacggata tcaacgggat cttcaccttt aagaatatca
     5101 gcctgtccag tcgcttcggc tttgtgaaag caagcaagac cggctatttc accggcagcc
     5161 gaagtgtcgc taccgacggc ggcgaatcca acttcgtttc catccaactg atcccccgca
     5221 atgaaacggg caatttcgcg gctgcttccg gtggcaaaat aatggtcttt tccggcgaca
     5281 ccgtatcctt tagcccttcc tccgtggtga acgcagttac caacgcggcc tatacgggca
     5341 ccgtacacgt cttcgccacc tatctcaacc caactaatga caaccttacc aaatatatgc
     5401 ccggcgacct tcgcggcatc agcaccagcg ggaaagaggt cgccctgcaa acctatggaa
     5461 tgatgagcgt cgaactgcag gacgacgccg gcaacaaact gcaactggct tccggtcagc
     5521 aggccacgat cacgatggcc atccccgacc tgctgcaaag cacggccccg gctaccattc
     5581 ccctctggta tttcaacgac agcaccggcc gctggatcca gcagggctcc gcgacccgcc
     5641 agggcaataa ctacgtggga agcgtgggcc atttcacctg gtggaact
//
LOCUS       DATGFG010000948         5061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_404723, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000948 DATGFG010000000
VERSION     DATGFG010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404723"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..413)
                     /locus_tag="VKQ52_17935"
     CDS             complement(45..413)
                     /locus_tag="VKQ52_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89144.1"
                     /translation="MKNLFVASRPSKAPFLPFEPTASRIKVSLHQLINDLLTGLQPLA
                     MKRNNVVLNGVPLGLCFVAEENLLAYALWNFLTGIITNKQNECIHVQTLVDDERTMIC
                     VREAGAYQVALSLSNHRMAV"
     gene            598..3288
                     /locus_tag="VKQ52_17940"
     CDS             598..3288
                     /locus_tag="VKQ52_17940"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HLZ89145.1"
                     /translation="MQKRALFFLFSFLSVITLSAQKAGSYRTGYDDSTLEQQSSFTLM
                     PFNRWIQSAGRVVTFGDPRLENHALDFAILPDSQHIAVEDRYGIAILDVRSQQIQQRW
                     KFQDDPAVRGLMSTYSGIKAFRYEGSSFIAWGAGSGRQSAVMIAGWDSSGIRDVRHIP
                     VDPIAPAGAALPNDIAVQVEGGVPYLYVVLNGNNQLLKIRFTDRQVVWAVPTGVAPFG
                     LRIVDNKVYVTNWAGPLVTDSSLEHAGTPWGSAYTNPVTGSTARGSLSVIDAQSGALL
                     SEVALGLHPNAIVSSPDSLYLYISNGNSDYVSVFDVRRGVVADSIPVGLFSGSITYYG
                     SSPNALQTDRAGTVLYVANGFDNAVCVIRLGSDAATNGRGRSVVRGYIPTEAYPGGVA
                     LLNNDLYIANIEAKGSRVLSPVSGIRGDDAASGGPVSAYTIHQELASLSIVPVPTEKE
                     LKAYTKKVREQSLFFRLAMTNRPARRHVTPVPVPERIGEPSVFKHVVYIIKENKTYDQ
                     VFGDIPQGRGMPQLCIYGDSVTPNQHRLAGAFSLMDNYYAAGKCSAEGHQWTDGAMVS
                     DYIEKSVRAWFRSYPHRQDDALVYNKNGFIWNNALDHGKKVRVYGEACTTKYDHALKW
                     NDIYQHYLNHEDQGFVNGTTIGRIRPIISPAYPDCDNLVFTDQLRADIFIDDWKRAEQ
                     GVGDSLPDLLVLSLPDDHTAGTSPNFPVPQAMVADNDLAVGRIVDAITHSRFWDSTVV
                     FITEDDSQSGWDHISSYRTTGLVISPYSVMGRAIHTNYNQTGMVRTIEQILGIPPMNV
                     IDATALPLFDCFSNQRSGYVFTHVPNVIPLDRMNKPLAALQGKAAYYARMSANQAFKD
                     VDGGDDELMNKILWFDARGEERYPDMGVVGRISGTGGPAK"
     gene            3327..4778
                     /locus_tag="VKQ52_17945"
     CDS             3327..4778
                     /locus_tag="VKQ52_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89146.1"
                     /translation="MKKNHLILAVFLLFGACKKNHDQQSAGGSNVPPYFEDILINETL
                     ANGSNAPFQRALGYFPLASDPGTQIMVASRGSEELNDARGFRIASINKSKGTVNWVRS
                     FDLPDQYFIQIATCAAIDANDNIWIGGHSFQGITPANLFLVKLDRSGNLIWSKSFSNY
                     QGFRAYSLAALSNGDIALFGKSGGAMVCHRLTADGNPVWSTQLVYTWWGTIDDDYYNK
                     PNNLSPENHGLVEGSDGSIYFATSSNSGTANVGGTDRLVRLDANGKILFANVYSLANN
                     TAVVRPVQLTAAGNGQLLLADQMVSYNGWEGCPYFTMLSGDGSVVASRGRPYDPNNVL
                     GITINEVNFYHDSVYYSTCGDNQFNTYILDAGLNLKSAVKTAATSDIGTDRGGISLYD
                     ATDRALYYVFNFGGNPGESNGFEVTRNGPTGKPCVTTYTIPPATLLLESTTVSVKADT
                     AVASSGYGPSPVFTSLTWRQYTVTTSPMNVCGQ"
     gene            complement(4814..>5061)
                     /locus_tag="VKQ52_17950"
     CDS             complement(4814..>5061)
                     /locus_tag="VKQ52_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89147.1"
                     /translation="GAELPGPDKSVSQLSVKNGRYYFFATQNNILKQTSDFATYTVIS
                     PPLSGPGDNAGIIKYFVTNDNHFILSTGLRGLYYQTP"
BASE COUNT         1068 a         1201 c         1606 g         1186 t
ORIGIN      
        1 catttgaata gcgaggggta tgcggtgttt gtggctttgc tgccttagac cgccatccgg
       61 tggttcgaaa ggctgagcgc aacctggtag gcgccggctt cccttacaca gatcattgtg
      121 cgctcatcgt cgaccagggt ctgtacgtga atgcattcgt tctgtttgtt ggtgatgatg
      181 ccggtgagga agttccagag tgcgtaggcg agcaggttct cctctgcgac gaagcacaat
      241 cccaggggga cgccgttgag gacaacgttg ttgcgtttca tggcgagggg ttggaggcct
      301 gtcagcagat cgttgatgag ctgatgaagg gagaccttga tgcgggaggc ggtgggttca
      361 aagggtaaaa agggggcctt ggaaggacgg gaagctacaa aaaggttttt cataaaataa
      421 cttgtgatgg tgaaagtggt tagcaaataa acaacgggtt tgcccttata cgatcaaatg
      481 ggggagggtt gttttatcat ttaagtataa acgacagtac cgataaatat ttctatgtaa
      541 agggaggcgc aattgggaat tgattttcag cttacatttg gcccttaaag tcaactaatg
      601 caaaagaggg cgcttttttt tcttttttcc ttcctttccg tcatcaccct gtccgcgcaa
      661 aaggccggat cgtatcgcac tggttatgac gacagtacgc tggagcagca aagctcgttt
      721 accctgatgc ctttcaacag atggatacag tccgcgggaa gggtggtaac ctttggcgat
      781 ccccgcctgg agaaccatgc cctggacttt gccattttgc cggacagtca gcatatcgcc
      841 gtcgaggacc ggtatggcat cgccatcctg gatgtccgga gccagcagat ccagcaacga
      901 tggaagttcc aggatgaccc ggcggtgcga gggctgatga gcacctattc ggggataaag
      961 gcattccggt atgaggggag ctcttttatc gcctggggcg ccggaagcgg ccggcaatcc
     1021 gccgtgatga tcgccgggtg ggattcctcc ggcatccggg atgtcaggca tatccctgtg
     1081 gatcccatcg cacccgcggg agccgccctg ccgaatgata ttgccgttca ggtggaaggc
     1141 ggcgttcctt acctctatgt ggtgctgaat ggcaacaacc agctgctgaa gatccggttc
     1201 acggacaggc aggtggtctg ggccgtgcct acgggtgtgg cgccgttcgg gctgcggatc
     1261 gtcgataata aagtctatgt gacgaattgg gccggaccgc tcgttaccga cagcagcctg
     1321 gaacatgcgg gtactccctg gggcagtgcg tataccaacc ctgtgacggg cagcacggcg
     1381 agggggagcc tatccgttat cgatgcgcag agcggggcct tgcttagtga ggttgcgctt
     1441 gggctgcacc ccaatgccat cgtcagcagt cccgatagtc tttacctgta tatcagcaat
     1501 ggcaacagcg attatgtcag cgtgttcgat gtgcggcggg gggtggtggc cgattccatc
     1561 ccggtggggc tgttctcggg cagtattacc tattatggca gcagtcccaa tgccctgcaa
     1621 acggataggg cggggacggt gttgtatgtc gccaatggat ttgataatgc ggtttgtgtg
     1681 atccggctgg gttcggatgc cgcgaccaat ggcaggggca ggagcgtggt gagaggatat
     1741 atcccaacgg aggcctatcc cggcggtgtc gccttgctga ataatgacct gtatatcgcc
     1801 aatatcgagg cgaagggttc gcgtgttctt tcgcccgtga gtgggatacg cggagatgat
     1861 gcggcctccg ggggaccggt gagcgcgtat accatccacc aggagttggc gtcccttagc
     1921 atcgtacccg tgccgaccga aaaggaattg aaggcctata cgaagaaggt gcgggagcag
     1981 agcctgttct tccggctggc catgaccaac cggcctgcgc ggcggcatgt gaccccggtg
     2041 ccggttcccg aaaggatcgg cgagccttcg gtctttaaac atgtggtcta tatcatcaag
     2101 gagaacaaga cctatgacca ggtgtttggc gatatcccgc agggaagggg gatgccgcag
     2161 ctgtgtatct atggcgacag tgtcacgccg aaccagcacc gccttgcggg cgccttttcg
     2221 ctgatggata attattatgc cgccgggaag tgttccgcgg aggggcatca gtggacggat
     2281 ggcgcgatgg tgtccgacta tatcgagaag agtgtgcgtg cctggttcag aagctatccg
     2341 caccggcagg atgatgcgct ggtctataac aagaatgggt ttatctggaa taacgcgctc
     2401 gatcatggca agaaggtgcg ggtatatgga gaagcctgta cgacgaagta tgaccatgcg
     2461 ttgaaatgga atgatatcta tcagcactat ctgaaccacg aggaccaggg gtttgtgaac
     2521 ggcacgacga tcgggaggat caggcccatt atttcgccgg cctatcccga ctgtgataac
     2581 ctggtgttta cggaccagtt gcgggccgat atttttatcg atgattggaa acgggccgag
     2641 cagggtgttg gggatagtct gcccgatctc ctggtgcttt cgctgcccga tgatcatacg
     2701 gccgggacgt cgccgaactt ccctgttccg caggcgatgg tggcggacaa tgatcttgcc
     2761 gttggcagga tcgtggatgc catcacgcac agtcgtttct gggacagcac ggtggtcttt
     2821 attacggagg atgattccca gtcgggctgg gaccatatct cttcttaccg gacgacgggg
     2881 cttgtcatca gtccgtattc ggtgatgggg cgggcgatac ataccaacta taaccagacg
     2941 ggtatggtgc ggacgatcga gcagatcctg gggataccgc cgatgaatgt catcgacgct
     3001 accgccctgc cgctattcga ttgtttctct aaccagcgat cggggtatgt gtttacccat
     3061 gtgccgaatg tcattccgct ggaccggatg aacaagccgt tggctgcgtt gcagggtaag
     3121 gcggcgtatt atgcccggat gtccgcgaac caggcgttca aggatgtcga tgggggtgat
     3181 gatgagctca tgaataagat cttatggttc gatgccaggg gtgaggagcg gtatccggat
     3241 atgggtgttg tgggtagaat ttccgggacc gggggtccgg cgaagtagga ggaatgggaa
     3301 ccgctttgta tattgtggaa accgttatga aaaaaaacca cctgatcctt gccgtattcc
     3361 tgctctttgg cgcctgcaaa aagaatcacg atcaacagag tgccggcggc agtaatgttc
     3421 ccccctactt cgaagacatc ctcatcaatg agaccctggc caatggcagc aatgcgcctt
     3481 ttcagcgggc gcttggttat ttcccgctgg cctcggaccc cggcacccag atcatggttg
     3541 cgagccgcgg atcggaggag ttaaacgatg ccaggggttt ccggatcgcg tccatcaata
     3601 agtcgaaggg gacggtgaac tgggtcagga gtttcgatct gccggatcag tattttattc
     3661 agatcgcgac ctgtgccgct atcgatgcaa acgataatat ctggatcggg gggcatagct
     3721 ttcaggggat cacgccggcg aatctatttc ttgtcaaact ggacagatcg gggaacctga
     3781 tctggagtaa gagtttcagc aattaccagg gtttccgggc ttattcgctt gccgcgctca
     3841 gtaacgggga tatcgccttg tttgggaaga gtgggggagc aatggtgtgt catcggttga
     3901 cggcggatgg gaaccctgtc tggtcgacgc agctggtcta tacctggtgg ggtaccatcg
     3961 acgatgatta ttacaacaag ccgaataacc tcagtcccga aaatcatggg ttggtggaag
     4021 gcagtgacgg cagtatttat tttgccacga gcagtaactc cgggactgcc aatgtcgggg
     4081 ggacggaccg gcttgtccgg ctggatgcca atgggaaaat tctttttgcg aatgtctatt
     4141 ccttagccaa taacaccgcg gtggtacggc cggtgcagtt gacggcggcg ggtaatggcc
     4201 agttgctgct ggcggaccag atggtcagct ataatgggtg ggagggttgt ccttatttta
     4261 cgatgttgtc gggggatggg agcgtggtgg cgtcgcgggg ccgtccctat gatccgaata
     4321 acgttctggg gatcaccatc aacgaggtga acttttatca tgacagtgtt tattattcga
     4381 cctgcgggga taatcaattc aatacctata tcctcgatgc cgggctcaat ctgaagagcg
     4441 ccgtcaagac cgccgccacg agtgatatag ggacggaccg gggcgggatc tcgctttatg
     4501 atgcgacgga ccgggccctt tactatgtgt tcaattttgg cggtaatccc ggcgagagca
     4561 atgggttcga ggtgacgcgc aacggtccca cgggcaagcc ctgtgttacc acctatacca
     4621 ttccgcccgc tacgctgctg ctggaaagta ccacggtgag tgtaaaggcc gatacggccg
     4681 tcgccagttc cggttatggg ccgtcgcctg tgtttacgtc cctgacctgg cggcagtata
     4741 cggtgacgac atcgcccatg aatgtttgcg ggcagtaggg agtgccggat tggccggtat
     4801 cgacgggcag gagttagggt gtctgataat agagtccccg tagtcctgtc gacaggatga
     4861 aatggttgtc gttggtgaca aagtatttaa tgatgccggc attgtctccg gggccggaca
     4921 aggggggact aatcacagta tacgttgcga aatcgcttgt ctgcttcagg atgttgttct
     4981 gggtagcaaa gaaataatag cggccgttct ttacggacag ctgggatacc gatttgtcgg
     5041 ggcccgggag ttcggcgccg g
//
LOCUS       DATGFG010000949         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_178576, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000949 DATGFG010000000
VERSION     DATGFG010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178576"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VKQ52_17955"
     CDS             complement(<1..1086)
                     /locus_tag="VKQ52_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HLZ89148.1"
                     /translation="MAEVILMPRLSDTMTEGVIAAWHKKVGDSVKKGELLAEVETDKA
                     TMDLESYKDGTLLYTGTDKGGKIQVNDLLAIIGAPGEDISALVKGGGAAAPAAPAASQ
                     APAPAAPAASPAPAAAPAASTTTAPAGGGIDLSKQEEVILMPRLSDTMTEGVIADWHK
                     KVGDTVKKGDILADIETDKATMELESYKEGKLLYIGAQKGEKIAVNDLLAVIGDEKKV
                     NLDQLISASKNKGVAGPVAAPAAQQAATAAAPAETAHSGLSVSADGRIKASPLAKKLA
                     AEKGIDISKVAGSGDNGRIIKKDIDSYTPSKAAPAAAAQPAATGKPTAPAVQGQESFE
                     EVPVSQMRKVIARRLAESKFSAPHFYVT"
     gene            complement(1167..>3192)
                     /locus_tag="VKQ52_17960"
     CDS             complement(1167..>3192)
                     /locus_tag="VKQ52_17960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ89149.1"
                     /translation="MLISEESYDSLPSRFVARKYGTSDSMKLVLTFDDGPDPDYTPKI
                     LDILSKYHVPAAFFVVGINAENNIPIVKREFKEGHEIGNHTFTHPNIAKVSTRRAILE
                     MESTRLLIECITGRSTIMFRAPYNADFEPEKWEELIPVAIARTKNYLDIGESIDPLDW
                     EPGTPADSIYERVIRRKDEMTKAGLSGNIILLHDAGGEGRESTVEALPRIIEYFQRRG
                     FRFTTVADILGKTKDEMMPPVPKGGGYYLLQINYYFAEIGYWGSHILFSAFVVFMLLS
                     VSRVLFIAFIASREHFREKKCGFRPFWSPDGKDAPLVSIIVPAYNEEVNAVGSVQSLL
                     NGDYPNYEIIFVNDGSNDQTYQRVSEAFADDPRVKVYTKPNGGKASALNFGISRSDAE
                     FVICIDADTKLKPNAVSLMMEHFGERTGRRSRQQGLVGAVAGNVKVGNQVNLLTRWQA
                     IEYISSQNFDRKAFSYLNAITVVPGAIGAFRKKAIEDAGGFTIDTLAEDCDLTIRILR
                     CGYVIENDNRAVAMTEAPETLKQFFKQRFRWSYGVMQTFWKNRDALMNWKYRWLGWAA
                     LPNILIFQYIIPFVIPLADFFMIVGLLTGNASKIGGYYLVFMLVDLAVALLAFSFERE
                     NLTRLVWLIPQRLIWRWLTWLVLFRAVRRALKGELQHWGVLKRTGNVKVV"
BASE COUNT          635 a          976 c          852 g          729 t
ORIGIN      
        1 cgttacatag aaatgcggcg cggagaactt gctttcggcc agccggcgcg cgatcacctt
       61 tcgcatctgc gagaccggta cctcttcgaa gctttcctgg ccttgtacgg cgggagccgt
      121 gggtttgccg gtcgccgccg gttgtgcggc tgccgccggc gccgccttgg agggagtata
      181 gctgtcgata tccttcttta tgatccgtcc gttgtcgccg ctgcctgcca ctttggagat
      241 gtcgatcccc ttttccgcgg ccagcttctt cgccagcggc gaggccttga tgcggccatc
      301 ggcactcacc gaaagcccgg agtgggcggt ttcggccgga gctgcagcgg tagcagcctg
      361 ttgagccgcg ggagcggcca cgggaccagc gacaccctta ttcttcgaag cggatatcaa
      421 ttggtccagg ttcaccttct tctcatcacc gatcacggcc agcagatcgt tgacggcgat
      481 cttctctccc ttctgcgcac ctatatataa cagtttgcct tctttatagc tttccagctc
      541 catcgtcgcc ttgtccgtct cgatatccgc caggatatcc cctttcttta ctgtatctcc
      601 caccttttta tgccagtcgg cgatcacgcc ttcggtcatg gtgtcgctca accggggcat
      661 caggatcact tcctcctgtt tggagagatc gatgccgccg ccggcaggag ccgttgtggt
      721 cgaagccgcg ggcgccgccg ccggagccgg gctggccgcg ggcgctgcag gggccggagc
      781 ctgacttgcc gcaggcgctg ccggggccgc agccccaccc cccttgacaa gcgcggaaat
      841 atcttccccg ggtgcaccga taatcgccaa caggtcattc acctggatct tgccaccctt
      901 gtccgtgccc gtatataata atgtaccgtc tttataactt tccaggtcca tcgtcgcctt
      961 atccgtctct acttccgcca gcagctcgcc ctttttgacg gaatcgccca ctttcttgtg
     1021 ccatgccgcg atcactcctt ctgtcatcgt gtcgctcagc cggggcatta aaataacttc
     1081 agccataaac aatttcatta tttggagccg tgaaattaag acaattttgc tattccacgc
     1141 cggtggaggg gggaattcgc taccggttaa accactttta cgttgccggt gcgcttcagc
     1201 actccccagt gttgcagctc tcctttcagg gcccggcgta ccgctctgaa cagcaccagc
     1261 caggtcagcc agcgccagat cagccgctgc gggatcagcc acaccagccg ggtaaggttc
     1321 tcccgttcga aggaaaaggc cagcagggcc accgcgaggt ccaccagcat aaagacgaga
     1381 tagtaacccc cgatcttcga agcattaccc gtcagcagac cgacgatcat gaagaaatcg
     1441 gccagcggga tgacaaaagg aatgatatat tgaaagatga ggatgtttgg caatgcggcc
     1501 cagcccagcc accggtattt ccagttcatc agggcatccc ggttcttcca gaaggtctgc
     1561 atgaccccat agctccagcg gaaccgctgt ttgaagaatt gcttcagggt ctcgggggcc
     1621 tccgtcatgg ccaccgcccg gttgtcattc tcgatgacat aaccacagcg caggatgcgg
     1681 atcgtgagat cacagtcctc ggccagcgta tcgatggtaa aacccccggc atcttcgatc
     1741 gcctttttcc ggaaagcgcc gatggcgccc ggaacaaccg taatggcgtt gagataggag
     1801 aaggcctttc ggtcaaaatt ctgactgctg atatattcga tggcctgcca gcgggtcagg
     1861 aggttcacct ggttaccgac cttcacgttt ccggcaacgg cgccgaccaa accctgctgg
     1921 cggctccggc gaccggtacg ttcgccaaaa tgttccatca tcagggaaac ggcattgggt
     1981 tttagcttgg tatccgcgtc gatgcagatg acaaattccg catcgctccg gctgatcccg
     2041 aaattcagtg cggaagcctt cccgccattc ggcttggtat agaccttgac ccggggatcg
     2101 tctgcaaagg cttcactcac ccgctgatag gtctggtcat tgctgccgtc attgacaaag
     2161 atgatctcgt aattgggata gtcgccattc agcaggcttt gcaccgaacc cacggcattc
     2221 acctcttcgt tataggcggg cacgatgata ctcaccagcg gcgcatcctt gccatccggc
     2281 gaccaaaaag gccggaaccc gcatttcttc tcgcggaaat gttccctgct ggcgataaaa
     2341 gcgatgaaca ggacccggct caccgacagg agcataaaaa cgacgaaggc ggagaacagg
     2401 atatggcttc cccaataacc gatctccgca aagtaataat tgatctgcaa aagataataa
     2461 ccgcctcctt ttggtacggg cggcatcatc tcatcctttg tcttgccgag gatgtccgcc
     2521 accgtcgtga accggaaacc ccttcgctga aaatattcga tgatacgggg taaggcctct
     2581 accgtcgatt cccggccttc accacccgcg tcgtgcagga ggatgatatt gccgctcagc
     2641 cccgcctttg tcatctcgtc cttgcgccgg atcacccgtt catagatgct gtccgcgggc
     2701 gtacccggct cccagtccag cggatcgatg gactcgccga tgtccaggta gttcttcgtc
     2761 cgggcaatgg ccacggggat cagctcctcc catttctccg gctcgaaatc cgcattatag
     2821 ggcgcgcgga acatgatggt gctccgcccc gtgatacatt cgatcagcag ccgcgtcgac
     2881 tccatctcca ggatggccct gcgtgtgctc accttggcga tattcggatg ggtaaaagta
     2941 tggttgccga tctcatgtcc ctccttgaat tcccttttta cgatcgggat attgttctcc
     3001 gcgttgatcc cgacgacgaa gaaggcggcc ggaacatggt acttgctgag aatatcgagg
     3061 atcttggggg tatagtcggg gtccggaccg tcgtcaaacg tgagtaccag cttcatggaa
     3121 tcacttgtcc catatttccg ggcgacgaac cgcgagggca gactgtcata gctttcctcg
     3181 ctgatgagca tc
//
LOCUS       DATGFG010000950         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1514063, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000950 DATGFG010000000
VERSION     DATGFG010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514063"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..2018
                     /locus_tag="VKQ52_17965"
     CDS             162..2018
                     /locus_tag="VKQ52_17965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ89150.1"
                     /translation="MKNARTPLPDGPITWKQRLMALSNLPRFFRLVWQTSPGIMLVNG
                     ILRITRSAIPVAILYVGKLIIDQVVLLLRSGQLTAAGVQGGGLSFLWKLVALEFGLAL
                     LSDGLMRAITLMDSLLGERFANFTSIKIMEHAASLDLDQFEDTTFYDKLERARQQTNG
                     RTVLLSQVLGQVQDLISMGFLAVGLMAFNPWLILLLLVAVVPAFLGESYFNDRSYALM
                     RAQTAERRELDYLRYLGASDETAKEVKLFNLSRFLIDRFQTLSDKFYRDARDLAVRRS
                     SWGVFFSMLGSLGYYSAYVFIILRAVHGRISIGDLTFLAGSFRQLRTLLEGILSRFTS
                     VSQGAIYLQDLFEFFEITPRIVRVAHPRPFPRPIRQGFTFEDVGFRYLHSERWANRHL
                     SFTLRPGERLALVGENGAGKTTLIKLLTRLYDPTEGRILLDGVDLREYDPDELRQQMG
                     VIFQDYLRYQMTMAQNIAVGNIVHKEDRTLIEAAAQQSLANVLAERLPGRYDQELGRR
                     FRQGVELSGGEWQKVALARAYMRDAQLLILDEPTAALDARAEYEVFQRFSELTKGRTA
                     VLISHRFSTVRMADRILVLDKGQLLEIGSHEELLRKGGRYAELFHLQAQGYR"
BASE COUNT          399 a          574 c          644 g          464 t
ORIGIN      
        1 tggtcaccgt atcgccgacg ctgatctggc cgagcgttct tacaccggct gccaatgtca
       61 ggaaaagaag gataatgatc gggagggctc ttttcatggt gtggttaaat gtaccgctta
      121 tttggagaac cgaaggccgg agattttgtt aacttacctg tatgaagaat gcaaggacgc
      181 ccctgccgga tggcccgatc acctggaaac aacgcctgat ggccctttcc aacctgcccc
      241 gcttcttccg gctggtatgg cagaccagtc ccgggatcat gcttgtcaac ggcatattgc
      301 ggatcacccg ttcggccatc ccggtggcga tcctctatgt cggcaaactg atcatcgacc
      361 aggtggtgct gctgctccgc agcgggcagc tcacggcggc cggtgtacag ggcggcggac
      421 tatcgttttt atggaagctc gtggccctgg aatttggcct ggcgcttttg tcggatggat
      481 tgatgagggc tattaccctg atggatagcc ttttgggaga gcgattcgcc aattttacct
      541 cgattaagat aatggaacat gcggccagcc tggacctcga tcaattcgag gatacgactt
      601 tttatgacaa actggagcgg gcgcggcagc agaccaatgg ccggacggta ttgctctccc
      661 aggttcttgg ccaggtgcag gatcttatca gcatgggttt tctcgcggtg gggctgatgg
      721 cattcaatcc ctggctgatc ctgttgctcc tggtggccgt cgtcccggct tttttagggg
      781 aatcttattt caacgaccgg agctatgccc tgatgcgggc gcagacggct gaaagacggg
      841 aactcgatta tctgcgttac ctgggagcca gtgacgaaac ggccaaggag gtaaagctgt
      901 tcaacctgtc ccgtttcctg atcgaccgtt ttcagaccct gtcggataaa ttttaccggg
      961 atgcccgtga cctggccgtg cgccgcagct cctggggtgt ctttttctcc atgctgggca
     1021 gcctgggcta ttacagcgcc tatgtcttta tcatcctgcg ggccgtccat ggccgcatct
     1081 ccatcggcga ccttaccttt ctcgccggct ccttccggca gctgcgaacc ctgctggagg
     1141 gcatcctcag ccggtttacc agcgtttcgc agggggctat ctatctgcag gacctcttcg
     1201 aattcttcga gatcacaccc cggatcgtgc gtgtggcgca cccccggccc tttccccgac
     1261 ccatccggca agggtttact ttcgaagacg tcgggttccg ataccttcat tccgaacgat
     1321 gggcaaaccg gcatctcagc tttacgctgc ggcccgggga aaggctggcg ctggtggggg
     1381 agaacggcgc cggcaagacc acgctgataa agctgctcac gcgattgtat gaccccaccg
     1441 aagggcgtat cctgttagat ggcgtcgatc tgcgggagta tgaccccgac gaattgcgtc
     1501 agcagatggg cgttatcttc caggactacc tccggtacca gatgacgatg gcgcagaata
     1561 tcgcggtggg caatatcgtg cacaaggagg accggaccct tatcgaggct gcggcgcagc
     1621 agagtcttgc caatgtgctg gcggagcggt tacccggccg ctatgaccag gagctggggc
     1681 ggcggttccg gcagggtgtc gagctgtccg gcggcgaatg gcaaaaagtg gcgctggccc
     1741 gggcctatat gcgggatgcg cagctgctga tcctcgacga gcctacggcg gccctcgatg
     1801 caagggcgga atacgaggtg ttccagcgat tctccgaatt gacgaaggga cggactgcgg
     1861 tactgatctc gcatcgcttc agtacggtgc ggatggcgga ccgtatcctg gtgctcgaca
     1921 aagggcagct gctggaaata gggagtcacg aagagctgct gcgcaagggc gggcgatatg
     1981 cggagctgtt ccatttgcag gcgcagggat accggtagca ggcgaaaggc cggccggctc
     2041 ttcaaggttg cttcaaaatc cttcgcgaat atagcagccg g
//
LOCUS       DATGFG010000951         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1400269, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000951 DATGFG010000000
VERSION     DATGFG010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400269"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VKQ52_17970"
     CDS             complement(<1..1207)
                     /locus_tag="VKQ52_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89151.1"
                     /translation="MKSFLLIVLLSGPAVLARSQSVGDVVKQQAGEGVKTGAAVATEQ
                     TANNVSNRLLNKLFNKKSKNAKADSVKNAGAAVPVAAVPGAGNGAGAAVASGSGVSGS
                     GVSGAGGVGGSGSGAAGGAGSAGLTTYSKFDFVPGDKILVVEDFGQDAIGDFPDKWNT
                     NSTGEVQGIQGKEGKWMSITKRGLFLPEFINSLPDNFTLQFDLLCSDNIVYGSGNFGV
                     SFDVIPNASKQFTQLGHAVPRNEAAVWFQPNAMGHGLTLFALFSDDVQTMNNEVTDMD
                     WVAVDAGKRAVKISFWRQRQRLRVYMNEEKVWDLPRAFETGKNYNTILFRLADDVLQN
                     GHYLFTNVRLAVGAPDTRTKLLTEGRFSTTGILFDVNSAVVRGESYGALKDIADVLKD
                     NGTLQVKIVG"
     gene            1447..>3002
                     /locus_tag="VKQ52_17975"
     CDS             1447..>3002
                     /locus_tag="VKQ52_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ89152.1"
                     /translation="MGKATAFILFLHLGLFLHPGLSALAQPFPALRWTQLTEKEGLSC
                     DKTTSVAQDGDGIIWVSTSNGLNRFDGYGFAKYFSKLDDTTSLLDNDIESIYADRRNN
                     LWLTTAVGVCRFNTITHKTTRFPSDTTAPPTFRSFDNPRVWFDEKEDPFIVSPSGLYH
                     FTDDAHYATLEEGFAPQLFQERLFTHYAALVRDNRGGLWSFQQNRLYKIDPVTKRVSR
                     SITAPGHFTIYDVVFDNHNRAWISTWSHGVYRFDPADDSWLLIPSDHGENVVKRGVEW
                     QLDGRRFIVFATNRPDLLFIDEETGKCRTYVPTGSGEYGIPFVDRQNILWIPTTKGLF
                     CVNSYSRLFEILPQHSPGLPDADSINWATPYNMREEKSGYWVASRYRGGMFWYDHNWR
                     LLHYWPMVVDSVGLPLQEEVATLQEAFDFKQLEDDMYITTEWGMMVMNLKTLRRKLIR
                     CPWTRPVMRLRTIAGDGKGKWWVRSFDQGVFVFDPMTQQFTRHYSLIKCSGCELPQVN
                     YLLRDHQGRV"
BASE COUNT          618 a          929 c          821 g          634 t
ORIGIN      
        1 gcccgacgat cttcacctgc agcgtgccat tatccttcaa cacatcggcg atatccttta
       61 gcgcgccata cgattccccc ctgacgacgg cggagttcac atcgaagaga atgccggtgg
      121 tcgagaacct gccttcggtg agcagcttgg tccgcgtgtc gggagcgcct accgcaagcc
      181 ggacattggt gaaaaggtag tgaccgtttt gcaggacatc atcggcgagc cggaagagga
      241 tcgtgttata gttcttcccg gtctcgaagg cgcggggaag gtcccatacc ttttcctcgt
      301 tcatatagac cctgagccgc tgacgctggc gccagaaaga gatcttcacc gcccgcttgc
      361 ccgcatcgac ggcgacccag tccatatcgg tgacctcatt gttcatcgtt tggacatcgt
      421 cggagaagag cgcgaacagg gtgagcccat gccccatggc attgggctgg aaccacacgg
      481 ccgcttcgtt gcgtgggacg gcatggccca attgcgtgaa ctgcttcgat gcattgggga
      541 tgacgtcgaa gctgacgccg aaattaccag agccataaac aatattgtcg ctgcaaagca
      601 gatcgaattg caacgtgaaa ttatccggca ggctgttgat gaattccggc aggaacaggc
      661 ctctttttgt tatcgacatc cacttgccct ccttcccctg gatgccctgc acctcccccg
      721 tgctgttggt gttccattta tccgggaaat cgccgatggc gtcctggccg aagtcctcga
      781 cgacgaggat cttgtcaccc ggcacaaagt cgaatttgct gtacgtggtg aggccggccg
      841 aacccgcgcc gcccgccgcc ccgctccccg aaccgccaac gccccctgcc cccgaaacgc
      901 cgctacccga aaccccgctc cccgaagcaa cagcggcccc ggcgccattg cctgcaccgg
      961 gtacggcggc caccgggacc gctgcgcctg cattttttac cgaatccgcc ttcgcattct
     1021 tgctcttctt attaaataac ttatttaaca accggttaga cacattattg gccgtctgct
     1081 ccgtggctac ggcggcgccc gtcttcacgc cttccccagc ctgttgcttt accacatcgc
     1141 cgaccgactg gcttcgggcc aggacggcag gtcccgacag aaggacgatg agaagaaatg
     1201 atttcatacg ctgtgttttt tcatggaaga cgaaaatacc gccccggcag gcaccatcgg
     1261 ggcggactgc gtatccggcg gaggggaata tatttccggc ggcattcggg cggaagatgg
     1321 tggggcggat tcgggaggag ctgccgtggc caaatatgga gccgccccca ccaattatgt
     1381 attgtgcctt tgtacagggg ttgcaaggcc gcggcctttc gcgtaatttg gagtacaatc
     1441 cagcgcatgg gaaaggcgac ggcattcata ctgtttctgc atctcggact ttttctgcac
     1501 ccggggcttt ccgcccttgc acagcccttc cccgccctgc gatggaccca gctcaccgaa
     1561 aaggaaggcc tctcctgcga caagaccacc tccgtcgcgc aggacggcga cggcatcatc
     1621 tgggttagca ccagcaatgg actcaaccgg ttcgacggct atggctttgc caaatacttc
     1681 tccaaactcg atgacacaac cagcctgctg gacaatgaca tcgagtccat ctacgccgat
     1741 cgacggaata atttgtggct gacgacggct gtcggggtct gccggttcaa taccatcacc
     1801 cataagacca cgcgtttccc gtcggacaca accgcccctc ccacctttcg cagcttcgac
     1861 aacccccgcg tctggttcga tgagaaagaa gaccccttta tcgtttcccc ctcgggactc
     1921 tatcatttta cggacgatgc ccactatgcg acactggagg aagggtttgc cccgcaactc
     1981 ttccaggaac ggctcttcac gcattatgcc gcactggtac gcgacaatcg cggcgggctc
     2041 tggtccttcc agcagaacag gctgtataag atcgaccccg tcaccaaaag ggtgtcgcga
     2101 tccattacag cccctggtca tttcaccatt tacgacgtcg tattcgacaa ccataaccgc
     2161 gcctggatca gcacctggag ccatggcgtc tatcgcttcg acccggcgga cgacagctgg
     2221 ctcctcatcc cttccgatca cggggagaat gtggtcaaac ggggcgtgga gtggcagctc
     2281 gatggccggc gctttattgt attcgccacc aacagacccg acctgctctt tatcgacgag
     2341 gagaccggca aatgccggac ctatgttccg acaggctcgg gtgaatacgg catccccttc
     2401 gtcgaccgcc agaatatcct ctggataccc acgacaaagg gcttgttttg tgtgaattcc
     2461 tatagccgcc tgttcgagat cctgccgcag cattcgccgg gcctgccgga tgccgatagc
     2521 atcaactggg ccacccccta caatatgcgg gaagagaaga gcgggtattg ggtggcttcc
     2581 cgctaccgtg gcgggatgtt ctggtatgat cataactggc gattgctcca ttactggccg
     2641 atggtggtcg acagcgtagg gctgcccctg caggaagagg tggcgacact acaggaagcc
     2701 ttcgatttca aacagctgga ggacgacatg tatatcacca ccgaatgggg gatgatggtc
     2761 atgaacctga agacgctccg gcgtaagctg atccgctgcc cctggacaag acccgtgatg
     2821 cggctgcgta ctatcgccgg ggacggcaag ggcaagtggt gggtccgctc tttcgaccag
     2881 ggcgtgtttg tcttcgaccc catgacacag cagtttaccc ggcactattc cctgatcaaa
     2941 tgctccggct gcgaattgcc gcaggtgaac tacctgctca gggaccatca gggccgcgtc
     3001 tt
//
LOCUS       DATGFG010000952         6101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_51816, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000952 DATGFG010000000
VERSION     DATGFG010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51816"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VKQ52_17980"
     CDS             complement(<1..813)
                     /locus_tag="VKQ52_17980"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ89153.1"
                     /translation="MKKIYTILWLAVVAATASSCHKFLDQQPNSEASDQTTWKSDGDA
                     NASVAACYSLIRSALNTSITHYAYGDLPTDEINNVVGGDPAFLDIMNVNWGIGIPSAN
                     TYDPRIKTRLYTNFYTGIAQSNRCLYFIANMPLAAFTGSDNASQQARKNHLLGEAYFT
                     RAFNYFYMARVWGDVPLVTAYIADASVAPQLPRSPQAKVLAQAISDLNLAAQYLDWKD
                     ESSVDRVVRADKGAVFALLAHIYAWQADYGNCDKACDSVINSGGYTLLNGGNY"
     gene            complement(843..4064)
                     /locus_tag="VKQ52_17985"
     CDS             complement(843..4064)
                     /locus_tag="VKQ52_17985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89154.1"
                     /translation="MNCLQRITLVLCLLTLIFHRAQAQAVQAGGIVRNETGQPIEGVT
                     VQEKGLPGNGTVTKADGSFSLTLRGSSHTIVISYIGYENQEWKANGKELDIRLKESVR
                     SGQDAVVIGYQSVKRRNLTAAVSSISGKDIQDIPEASFDQMLQGRLAGVSVLSSSGEL
                     GAKVSVVIRGATNVDYGNANGGNTGPLYVIDGVIYDVNTIIPAYNSQNAITGAVTTTN
                     PLSLINPNEIESIDVLKDASAAAIYGARAGNGVIIVKTKRARRGKPQVTASAYLGATT
                     HPAFRQVYTGDAERALKLDLLNAQLPYTSLQLGTLPIALTDSLNPAFNNDVDWQGLMI
                     RNNALVNNEDIGVAGFSGSTSYRLSLNHYNEQGALNGFSVERFAPHLALGINPVKNLN
                     IRADLLISSEKRRHGTGGSSGTLFSSWNFPSSFVQLSPAQQATFKGQANPFDDNGIFA
                     MNASLGLTDTIAHNLLFNSTYSASSYSDRWDYFSPAVLNGVLNTAYDVNSSNPSWTFE
                     NYLTYMRSFNKSHLVLVGGVSAYDVKNYFTNASAAGIAVSGITTLQTVPPGTNLSVTT
                     SRQEKTTVSYYGRLSYDYDGKYLATASLRKDASSIYSSSYQWGTFPSFSAGWIASGED
                     FFEPVKDVVNFFKLRASYGITGNDPGTFYAKYQSLSPDASYFGSTTGALGTGTNPSLA
                     GVPSTYNGTTTLSPYPYQTGFNNVGVNSSKSVRWEKFPQWDVAADMEFFNSRINLSVD
                     LYQKDALDKYFYNIPAQVTTGYDFYSGNFVNVRNQGIEIALATRNLSPKSAFQWNTTF
                     NIAFNRNFVTKLPNGNRDFLFGPPWFQQSLTIGQPLFNYKVWEKNGTYATDADVPVDP
                     ITGKKMTFQGVPLQAGDARYVDINGDYNIDYSDKVIAGNPNPKATGGFGNTFTYKGIS
                     LNIFASFVLGRKIFNGYLSDALNGSNAYQGAWTANAGPASIPSLLGQFWTKPGDNTRF
                     PRLVYPNGTAQDPWNIASSFFVEDGSFLKVKQVTLAYNLPEKWASRLHMRFINVYGMA
                     ENLLMLKKSKMIPDPELVDPTTGTANVVYPSALKLTAGFRVEF"
     gene            complement(4181..>6101)
                     /locus_tag="VKQ52_17990"
     CDS             complement(4181..>6101)
                     /locus_tag="VKQ52_17990"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HLZ89155.1"
                     /translation="DWMKYTCTLIAGHSDPRAQFVLSFGGAGSLWLDFVSLFPEKTFK
                     GRPNGLRADLAQYIADLHPSFVRWPGGCFVEGINIENAPDWKHTLGPIEKRPGTYSPW
                     GYWSSDGFGYHEYLQFCEDIGAAALYVFNAGVSCEFRSGTFTPDDSLGPVIQNALDAI
                     EYAVGPTNSRWGRVRASNGHPAPFPLKYVEVGNEQHGPRYARRYNRFYTAIREKYPGI
                     SIIASMGIADVNRNTLDSMQQVDITDEHAYKGAGWSFTHYDHFDKYKRGKWDMYVGEY
                     ATNGGVGKGNMAAALGDAVYLMAMERNSDLVKMSSYAPLLVNVNDESWPVNLINFDAG
                     NSFARISYYAIRMFNENKPDINLETTIQMQPAADTAKTYCFSGGIGLATWDTQSEYKD
                     MEVQQNGQVVYKADLINHPADWQSIRGDWTIGDSAVKQSAAGAQLLALLKDRTFDTYT
                     LTLKARKIDGYNAFIIPFAVKDSNDCLRAHIGSYVNSHCVFEKVTGGYEVTDLTDQRR
                     MKQPIEKNRWYAIRLEVQPDKVDCYLDGELLMTYKQPDRLFAISGRDEKTGDIIVKMV
                     NTGGTPVTTAIQIAGNSRTDNKAILTTLAAERENAENSFEYPTAHIPVQSEDNSNALQ
                     LSLKPYSVNVLRIKK"
BASE COUNT         1306 a         1538 c         1708 g         1549 t
ORIGIN      
        1 gtaattacca ccgttcagca gggtataacc accggaattg atgacggaat cgcaggcctt
       61 gtcgcaattg ccgtaatcgg cctgccaggc gtaaatatgg gctaataggg cgaatacggc
      121 tcctttatcg gcgcgtacca ccctgtcgac ggaggattcg tccttccagt ccaggtattg
      181 ggccgccagg ttcagatcgc tgatcgcctg tgccagcact tttgcctgcg gcgatctcgg
      241 caattgcggc gctacggaag catcggcgat atacgcggtc accaggggaa cgtctcccca
      301 aacccttgcc atataaaaat aattgaaggc acgggtaaaa taggcttccc ccagcaagtg
      361 gttcttgcgg gcctgctggc tggcgttgtc gctgccggta aatgcggcca gcggcatatt
      421 ggcgataaaa tagagacagc ggttactctg agcgatgccc gtatagaaat tcgtatacag
      481 ccgcgttttg atccttgggt cataggtatt ggcggaggga atgccaatgc cccagttaac
      541 gttcataata tccaggaagg ccggatcccc gcctactaca ttattgatct catccgtggg
      601 caaatctccg tatgcataat gggtgatcga cgtattcaat gcagaccgga tgagggagta
      661 acaggcggct accgatgcat tagcgtcccc gtcggatttc caggttgtct ggtcgctggc
      721 ctcgctgttg ggctgctggt ctaaaaattt gtggcaggag gaggctgttg ccgccacgac
      781 cgccaaccac agtatcgtat atatcttttt catgtaattg acttttgtta tttggcaaat
      841 gatcagaatt ccacccgaaa tcccgcggtg agcttcagtg cagaaggata aaccacgttg
      901 gccgttccgg tcgtgggatc gaccagttcc ggatccggta tcattttact tttcttgagc
      961 atcagcaggt tctccgccat tccgtagaca ttgataaatc gcatgtgcag gcggctggcc
     1021 catttttcgg ggaggttgta ggcaagggtc acctgtttga ctttcaggaa gctaccgtct
     1081 tctacgaaaa aggagctggc gatattccag ggatcctgcg cggtgccgtt agggtatacc
     1141 agccggggaa acctggtgtt gtctcccggc tttgtccaga attgccccag caggctggga
     1201 atggaggcgg ggccggcgtt ggcggtccag gcgccctgat aggcgttgct tccgttcagc
     1261 gcatcggaaa gatacccatt gaatattttc cttcccagca caaacgaggc gaaaatatta
     1321 aggctgatgc ccttataagt aaacgtgttg ccaaaaccgc ctgtggcctt cgggttaggg
     1381 ttgccggcga tgaccttatc gctgtaatcg atattatagt cgccgtttat atcgacatat
     1441 ctggcatcgc ctgcctgtag gggcacgccc tgaaaagtca ttttcttacc ggtgatcggg
     1501 tcgaccggca catcggcatc cgtcgcgtag gtgccgtttt tctcccacac tttataattg
     1561 aacagcggct ggcctatcgt gagcgattgc tggaaccatg gcggtccgaa aaggaaatcc
     1621 cggttgccat tcggcaattt ggtgacaaaa ttcctgttga aggctatatt gaaagtcgta
     1681 ttccattgaa aggcagattt ggggctcagg ttccttgtgg cgagcgctat ctcgatgccc
     1741 tggttcctta cgttgacgaa gttgcccgaa tagaaatcgt accctgtggt aacctgcgcc
     1801 ggtatgttat aaaaatattt atcgagggcg tccttctggt acaggtccac tgaaaggttg
     1861 atccggctgt tgaagaattc catgtccgcg gctacatccc attgtggaaa tttctcccag
     1921 cggacgctct tgctggaatt gacgccgacg ttattaaaac cggtttgata ggggtacggg
     1981 gatagggtgg tcgttccgtt gtaggtggat ggaacaccgg ctaagctggg atttgtaccg
     2041 gtgcccaggg cgcctgtcgt ggacccaaaa tagctggcat ccggggagag gctctggtat
     2101 ttggcataga aagtaccggg gtcattcccc gtgatcccgt aactggccct cagcttgaag
     2161 aagttgacca cgtctttgac aggctcgaag aaatcttctc cggaagcgat ccagcccgcg
     2221 ctgaaggaag gaaaagtacc ccattggtag gaggaactgt agatcgagct ggcgtctttc
     2281 cggagggatg ccgttgccag gtatttgccg tcgtagtcat aagacagccg cccgtaatag
     2341 gatacggtcg tcttttcctg ccgggaagtc gttaccgata aattggtccc cggcggcacg
     2401 gtctggaggg tggtgatgcc cgatacggca ataccggccg cggaggcatt ggtgaaatag
     2461 ttctttacgt cataggcaga gaccccgccg accagcacca ggtgactctt attaaacgat
     2521 ctcatatagg tgaggtaatt ctcaaatgtc caggaagggt tggacgaatt gacatcatag
     2581 gccgtattca ggacaccatt caggacggca ggagaaaaat agtcccagcg gtcggaatag
     2641 ctactggccg aataggtgct attgaaaagc aggttatgcg cgatggtatc ggtcagtccc
     2701 agggaggcat tcatggcgaa gatgccgttg tcatcaaagg gattggcctg gcctttgaag
     2761 gtagcctgtt gtgccgggct cagctgaacg aaagaactgg gaaaattcca ggaggaaaaa
     2821 agcgtaccgg aagaccctcc cgtgccatgt cgtctttttt cagaactgat caggaggtcg
     2881 gcccgtatgt taaggttctt aaccgggttg atacccaacg ccaggtgagg ggcaaatcgc
     2941 tccacggaaa agccgttcag ggctccctgt tcgttataat ggttgaggga caaccggtag
     3001 ctggtggaac ccgaaaatcc ggctacgccg atgtcctcgt tgttcaccag ggcattattc
     3061 cggatcatca ggccctgcca gtccacgtca ttattgaaag cggggttcag gctatcggtg
     3121 agggcgatcg gaagtgtacc caactgtagg gaagtatagg gcagttgcgc attcagcagg
     3181 tccagcttca gcgctcgttc cgcgtcgccg gtatacacct gccggaaggc gggatgggtc
     3241 gtagcgccga gataggcaga cgcggtgacc tggggtttgc ctcgccgggc cctttttgtc
     3301 ttaacgataa taactccgtt gcctgccctt gcgccgtaga tggcggcagc ggaggcatct
     3361 ttcaggacgt cgatggactc tatctcattg ggattgatca ggctcagcgg gtttgtggtc
     3421 gtcaccgcgc cggtgatggc attttgggag ttgtaggccg ggatgatggt atttacgtca
     3481 tagatgactc cgtctatgac atagagggga cccgtattgc ctccgttggc attgccatag
     3541 tccacattgg tagcccccct gatgacgacg ctaaccttgg cccccagttc accggagctg
     3601 gatagcacgc tgacgccggc cagccgtccc tggagcatct ggtcgaagct ggcttcgggg
     3661 atatcctgta tgtctttacc gctgatggag gatacggctg cagtcaggtt gcgccgcttc
     3721 acactttgat acccgatgac cacggcgtcc tgtcctgacc ggacactttc ctttaaccgg
     3781 atgtccaact cttttccgtt ggctttccat tcctggttct catacccgat gtaggagatg
     3841 acgatggtat gagaagatcc ccggagtgta agggaaaagg agccatcggc cttcgtgacg
     3901 gtgccatttc ccggcagtcc tttttcctgt acggtgactc cctctatggg ctggccggtt
     3961 tcgttcctga caatgccccc cgcctggacg gcctgggctt gcgcccggtg aaagatcagc
     4021 gtaagcaggc ataatacgag ggtaattcgt tgtaagcagt tcataagact agttttggtg
     4081 aaacaagatt attacttgtc atttagacat ttaaaactaa tcatatagaa agtctcccca
     4141 ttctcgtatt ttaagaattt cttatctgat tttaacccgt ttattttttt atccgaagga
     4201 cgtttactga atagggcttc agtgacagtt ggagtgcgtt ggagttatcc tccgattgga
     4261 cgggaatatg ggcggtgggg tattcaaaag aattttctgc gttttcgcgc tctgcggcca
     4321 gggtggtcag tatggccttg ttgtccgtgc ggctgttccc tgctatttgt attgcagtcg
     4381 tgacgggcgt tcctcctgta tttaccattt taacaatgat atcgccggtc ttctcgtccc
     4441 ggccggaaat agcaaaaagc ctatccggct gtttgtatgt cattaacagc tcaccgtcca
     4501 ggtagcagtc cactttatcc ggctgtactt ccaggcgaat ggcgtaccag cggtttttct
     4561 cgatgggttg cttcatccgc cgctgatcgg tcaggtccgt tacctcgtag cctccggtga
     4621 ctttctcaaa aacacaatgg ctgttaacat aggatccgat atgcgcccgc aggcagtcat
     4681 tgctgtcctt tacggcgaag ggtatgatga atgcgttgta accatcgatc ttacgggctt
     4741 tcagcgtaag cgtgtatgta tcgaatgtcc tgtctttcag cagggccagt aactgcgctc
     4801 ctgccgcaga ttgtttaacg gcgctatccc ctatcgtcca gtctccccgt atggattgcc
     4861 aatcggcagg atggttgata agatcggcct tgtacacgac ctgtccgttc tgttgcacct
     4921 ccatgtcttt gtattcgctt tgcgtatccc aggtggccag tccgatcccc ccggagaagc
     4981 agtaggtttt ggccgtatcg gcggcgggtt gcatctgtat cgtcgtttcc aggttaatat
     5041 ccggtttgtt ctcattgaac atccggatag cgtagtagga aatacgggcg aagctattgc
     5101 cggcatcgaa gttgatcagg ttgaccggcc aggactcatc gttcacattg accagcaggg
     5161 gtgcatagct gctcattttg accaggtcgg aattcctttc catcgccatc aggtagacgg
     5221 catcgccgag tgcggcggcc atattacctt ttcccacgcc gccattggtg gcatattcgc
     5281 caacatacat atcccatttg cctcttttat atttatcgaa atgatcataa tgggtaaaag
     5341 accagccggc gcctttatag gcatgttcgt ccgtaatgtc cacctgctgc atactatcca
     5401 gggtattccg gttgacgtcc gcgatgccca tgctggcgat gatgctgata ccggggtatt
     5461 tttcccggat cgcggtatag aaccggttat accgccgtgc ataccgggga ccatgctgtt
     5521 cgttgcccac ttcgacatat tttaacggga atggggcggg gtgcccgttg gatgcccgta
     5581 ccctacccca tcgggagttg gtggggccga cggcatattc aatggcatcc agtgcgtttt
     5641 gaatgacggg tcccaggcta tcatccggtg taaaggttcc gcttcggaat tcgcaggaga
     5701 cgccggcatt gaatacatag agtgccgcgg cgccgatgtc ttcacaaaat tgcaggtatt
     5761 catgatagcc gaatccgtca ctggaccagt agccccaggg gctgtacgtg cccggtcttt
     5821 tctctatggg ccccagggtg tgtttccaat ccggggcgtt ctctatattt atcccttcca
     5881 caaaacaacc tcccggccag cggacaaagg aaggatgaag atcggcgata tattgggcca
     5941 ggtcggcccg caggccatta ggccggcctt tgaaggtctt ttcggggaaa agggagacaa
     6001 aatcgagcca tagggaacca gcaccgccga acgacaatac aaactgcgcc cgggggtccg
     6061 agtggccggc aattaaagtg caggtatatt tcatccagtc t
//
LOCUS       DATGFG010000953         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1544430, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000953 DATGFG010000000
VERSION     DATGFG010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544430"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            596..1549
                     /locus_tag="VKQ52_17995"
     CDS             596..1549
                     /locus_tag="VKQ52_17995"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HLZ89156.1"
                     /translation="MQHLAKIFSGTGSQYLAEKIAQQFGHPSGKITIQHFSDGEIQPI
                     FNESIRGDMVFLVQSTCAPAENLLELLLMIDAARRASAYKVVAVIPYYGYARQDRKDK
                     PRVAIGSKLIANMLVSAGADRVITMDLHAPQIQGYFDIPVDHLDSSAIFIPYIEQLPI
                     QNLTFAAPDVGSANRIREIASYFNAEMVICDKHRKRANEIASMVVIGDVTDKDIVLID
                     DICDTGGTLAKAAGLLKEKGARTVRALCTHPVLSGKAYENIENSVLEELVVCDTIPLK
                     RQSPKIKVLSVAELFAVAIRNAHENRSITGLFIHSQLKEKR"
     gene            1592..2248
                     /locus_tag="VKQ52_18000"
     CDS             1592..2248
                     /locus_tag="VKQ52_18000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HLZ89157.1"
                     /translation="MKTITIEGQLRTEIGKQATRQLRSEEKVPGVIYGGAKEVNFSAP
                     LTSFKTLVYTPDFQLAEIKVNGQSYKCILKDLQFDKVSDTLIHADFLELVEDKKVIAT
                     IPIKFTGAAKGVKDGGKLITKMKTLKVKTYPKYLRENIEVDLTELELNGNVRVEDVKA
                     ENYEILNSPRIPIASIVLTRQLKQEEAAATTPAAGAKPAAAAPAAAAPAAAKPAAAKK
                     "
BASE COUNT          614 a          592 c          552 g          528 t
ORIGIN      
        1 cactcaaggc agcggccccc ttgttgatca acttttccct cggtctgtcg tccggcgccc
       61 aatactttat cggtgttttt ttcccttcca taaaatcccg tattcccttg caaatatcga
      121 aaattagcat tattttgtga ttctaacggc gggatgaaat aaccctttcg ttcctccgtt
      181 ttatgatccg gtacagacag ttgttcgcaa ctgtatcctt ttgaaaacta atctaaaatc
      241 caatgaatcg aactaacttt aacgcaatcg gcggctggca gtccttcttt ttctgtgttg
      301 gcatgtacgt agtcgcactc ttcttctcca tcttcgtttg ctcctccatt ttttacgctt
      361 ttaatgccaa gtctcaggcg aaagcagccg agaagaccgc tatgaccagt ccggcaacac
      421 aacagctggc gtcggttagc cactaacgaa ggatagctca aaatgcattg aggccggctc
      481 cgttcaacag aacggaggcg gccttttttt ggtcttactc taaaggtttg accttatttt
      541 tttgccttcc tgtattaata ggttacagta tcttcgccca tattttgtca catctatgca
      601 gcatctagcc aagatatttt ccggcacggg ttcccaatac ctggccgaga aaattgcaca
      661 gcaattcgga catccatcgg gaaagatcac cattcagcac ttcagcgacg gcgagatcca
      721 acctatcttt aatgaaagca tccgcggcga catggttttc ctggtacaaa gcacctgcgc
      781 cccggccgag aacctgctcg aactgctcct gatgatcgat gccgcccgcc gggcctcggc
      841 ctataaagtg gtcgccgtca tcccctatta tggctatgcc cgccaggaca gaaaggataa
      901 accccgggtg gcaattggta gtaagttgat tgccaatatg ttagtgtccg ccggcgccga
      961 ccgggtcatc accatggacc tccacgctcc ccagatacag ggatatttcg atatccccgt
     1021 ggatcacctg gacagcagcg ctatatttat tccctatatt gagcaacttc ccatacaaaa
     1081 tcttaccttt gcggcccctg acgtgggaag tgccaaccgc atccgggaga tcgccagcta
     1141 cttcaatgcc gagatggtta tttgtgacaa gcaccggaaa cgggccaacg aaatagccag
     1201 catggtggtc atcggggatg taacggacaa ggatatcgtc ctgatcgatg atatctgcga
     1261 cacgggcgga accctggcaa aagccgccgg tttgttgaag gagaagggcg cgcgtacggt
     1321 aagggccttg tgtacacacc cggtgctcag tggtaaagca tatgagaaca tcgagaacag
     1381 cgtgctggag gaactggtgg tttgcgatac gataccgctg aagcggcaaa gcccgaaaat
     1441 aaaggtgcta agcgtggcgg aactattcgc ggtagccatt cgcaacgcac acgagaaccg
     1501 gagtataacg ggcctgttca ttcactcgca actgaaggaa aagcgttgag cggggcggaa
     1561 aaaagagcat aattcttaaa caataataaa aatgaagaca attacaatcg aaggacaact
     1621 caggaccgaa attggcaaac aagccacccg ccagcttcgc tctgaggaga aagtgcccgg
     1681 tgtaatttac gggggtgcaa aagaggtgaa tttttcagct cctttgacgt cgtttaaaac
     1741 actggtgtac acacctgatt tccaattggc cgagatcaaa gtaaatggcc aatcctacaa
     1801 atgcatcctg aaggacctgc agtttgacaa ggtgtccgac accttgatcc acgcggactt
     1861 cctggagctg gtggaagaca agaaggtgat cgcgaccatt ccgatcaaat tcaccggtgc
     1921 ggcaaaaggc gtaaaggatg gcggtaagct catcaccaag atgaaaacac taaaggtaaa
     1981 gacttacccc aagtacctta gggagaatat cgaagtcgat cttactgaac tggaattgaa
     2041 cggcaacgta cgcgtcgaag atgtaaaggc ggagaattat gagatcctca actctccccg
     2101 gatccccatc gcatcgatcg tcctgacccg tcagttgaag caggaagaag cagctgcaac
     2161 aacccctgcg gcaggagcta agccagccgc cgcggcgcct gcagcagccg ctcccgcagc
     2221 agcaaaacct gcggcagcga aaaaataaat tattttatta gtaggaaccc ggttaaagtt
     2281 taaccg
//
LOCUS       DATGFG010000954         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_280949, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000954 DATGFG010000000
VERSION     DATGFG010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280949"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VKQ52_18005"
     CDS             <1..619
                     /locus_tag="VKQ52_18005"
                     /inference="COORDINATES: protein motif:HMM:NF020340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1793 domain-containing protein"
                     /protein_id="HLZ89158.1"
                     /translation="LEVHGFDPVNQLCTDDFAGHLARNANLSVKAIVALGAYGWMAGQ
                     LGQEAEATRYRQLAAEMAKKWMVLADAGDHYSLTFDRKDSWSQKYNLVWDKLLGLKLF
                     PPEVYDKEVAWYLSHQEPFGLPLDSRKTYTKSDWVCWTATLASRPEDFSALTDPLYRY
                     ITETPTRVPLSDWHETTNGRQVGFQARSVVGGYFIRLLDYQWNNR"
     gene            648..2939
                     /locus_tag="VKQ52_18010"
     CDS             648..2939
                     /locus_tag="VKQ52_18010"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HLZ89159.1"
                     /translation="MRKLFSFWAMALPLVMAAQAQDTASLVQYVHPMIGTQRMGHVYP
                     GATSPFGAVQLSPDTDTIPYEVNGKYNPDVYKYCAGYQYDDKTIVGFSHTHFSGTGHS
                     DLGDFLVMPTIGELKLNPGVAAVPHSGFRSAFSHATEVAEADYYRVKLEDYNILAELT
                     TTTRVGFHQYTFPATDQAHIVLDLMHGIYNYDDKNVWTYVRVVNDTLVTGYRQTSGWA
                     RTRTVYFAMVFSKPFYQYGARKYDRRQAYHGFWGHFDQNHDFPEEAGRQLRMYFDFRT
                     LAEGEKVKVKFALSPVSMDGALLNLRTEIPGWDFEAVKRQGQEAWNRELSKVRIDAGK
                     AEKENFYTSMYHAFINPTVYMDVDGGYRGLDQQNHVADGFINYTTFSLWDTHRALHPL
                     FNILQPARNADMIRSMLVHYQQSAEHMLPIWSHYGNENWCMSGYHSVSVICDAIVKGN
                     APFDANKALDACIATSTRRSFEGLGVYIDKGYIPAGQDGVSVSSTLEYAYDDWCIAQA
                     AKKLGRMDVYATYSKRAENYKNVWDPSIGYMRPRLADGSFRKDFDVLSTNGQGFIEGN
                     AWNYSLYVPHDPRALINLYGGNKSFVRHLDSLFTMELPDRFFAETEDITRDGIIGNYV
                     HGNEPSHHVAYLYNWTDQPWKTQERVRMILKKQYAPAPDGLGGNDDCGQMSAWYIFTA
                     LGFYPVAPGSDVYSLGSPAVNGAVIRLDNGKTFTIEADNQGDKNVYVKKVTLNGQPLT
                     GHTITHEEIMNGGKLVFFMSAKH"
     gene            complement(3217..3702)
                     /locus_tag="VKQ52_18015"
     CDS             complement(3217..3702)
                     /locus_tag="VKQ52_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89160.1"
                     /translation="MMRYAVLALLTSLLLTNCSKNNSSTNSQGTLSATVGSSAFTANQ
                     TFGAYYSSLSTMTVIGLVPQGKDSTILQLSLPYPLTVDTTRSSDNTSFGLSYYTRSGS
                     KSYDAFAGNGHAIFNISLFDTVNHKLSGTFSGTLLNDLNPSTDSVVITNGKFTTAYSV
                     Q"
     gene            complement(3838..4329)
                     /locus_tag="VKQ52_18020"
     CDS             complement(3838..4329)
                     /locus_tag="VKQ52_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89161.1"
                     /translation="MQIKHLARLSLSAVTLFVLITSCKKSNNTPSNSLSATIGTTGVN
                     TSGTTAWYSTDSSIYEIGGYTMSAHDTTTLALIIAPPFTVNTVVSGNSSVSIDYYDFA
                     TNKDYFAGYGVGHSALTVTSLDTVNHKIAGNFTATLYNGISGTDSVLVTNGKFNTSYT
                     VQP"
BASE COUNT         1019 a         1058 c         1368 g         1057 t
ORIGIN      
        1 tctcgaggtg cacggtttcg atcccgtcaa tcaattgtgt acggatgact ttgcgggcca
       61 tctggcgcgc aacgccaatc tttcggtaaa ggcgatcgtc gcgcttggcg cttatggctg
      121 gatggccggt caactggggc aggaggcgga ggcgacgagg taccggcagc tggccgcgga
      181 gatggcgaag aagtggatgg tgcttgccga tgcgggagat cactattcgc tgaccttcga
      241 ccggaaggac agctggagcc agaaatacaa tcttgtatgg gacaagctgc tggggttgaa
      301 actatttccg cccgaggtct atgacaaaga ggtcgcctgg tatctttcac accaggagcc
      361 ctttggcctg ccgctcgaca gccgcaagac ctatacgaaa tcggactggg tctgctggac
      421 ggcgaccctt gcctccaggc cggaggattt cagcgccctg accgatcccc tgtatcgcta
      481 tattaccgag acccccacgc gggtgccgct cagcgactgg catgagacga ccaatggccg
      541 gcaggttggg ttccaggccc gcagcgtagt gggcggctac tttatccggc tgctggatta
      601 tcaatggaac aacagataga taacttgtca aattttcttt gaatagtatg cgcaaactct
      661 tttctttttg ggccatggcc ctgccgctgg ttatggcggc gcaggcccag gatacggcca
      721 gcctcgttca gtatgtacac ccgatgatcg gaacgcagcg gatggggcat gtgtatcccg
      781 gcgccacttc gccttttggg gcggtgcagt tgagcccgga caccgacacg atcccttacg
      841 aggtgaacgg caaatacaat cccgatgttt ataaatactg cgccgggtat caatatgatg
      901 acaaaacgat cgtcggcttc agccataccc atttcagcgg taccggtcac tccgatctgg
      961 gcgatttcct ggtcatgccg acgatagggg aactaaagct gaatccgggt gtcgctgccg
     1021 tgccgcacag cggtttccgt tcggccttta gccatgccac ggaagtggcc gaagccgact
     1081 actaccgggt gaagctcgaa gattataata tccttgccga actgacgaca acgacgcggg
     1141 tgggttttca tcagtatacc ttccctgcca cggaccaggc gcatatcgtc ctcgacctca
     1201 tgcatgggat ctataactat gacgataaga atgtatggac ctatgtgcgg gtcgtgaacg
     1261 atacccttgt aacggggtat cggcagacga gcggctgggc gcgtacccgg acggtttatt
     1321 tcgccatggt gttctcgaaa cccttttacc aatacggtgc gcggaagtat gacagaaggc
     1381 aggcttatca cggcttttgg ggacatttcg accagaacca tgatttcccc gaggaggccg
     1441 gccggcagct gcgaatgtat ttcgacttcc ggacgttggc ggagggagag aaggtgaagg
     1501 taaaattcgc cctttcgcct gtcagtatgg atggagcatt gctgaatctt cgcacggaga
     1561 tcccgggctg ggatttcgag gccgtgaagc ggcaggggca ggaggcctgg aacagggaat
     1621 tgagcaaggt gcgcatcgat gcgggcaagg cggagaagga gaatttttat acttcgatgt
     1681 atcatgcctt tatcaacccc acggtgtata tggatgtcga cggcggctac cgcgggttgg
     1741 accagcagaa ccatgttgct gacgggttta tcaactacac gacgttttcc ctttgggaca
     1801 cccatcgggc cctgcatcca ctgttcaata tcctgcagcc cgcgcggaat gcggatatga
     1861 tccggagcat gctggtccat tatcagcaga gcgccgagca tatgctgccg atctggtcgc
     1921 attatggcaa tgagaactgg tgtatgtccg ggtaccattc ggtgtcggtg atctgcgatg
     1981 cgatcgtcaa gggcaatgcg cctttcgatg ccaacaaggc gctggatgcc tgtatcgcaa
     2041 cgtcgacgcg gcgcagcttc gaggggcttg gtgtgtatat cgacaaaggc tatatcccgg
     2101 ccggacagga tggcgtatcg gtgtcttcga cgctggaata cgcctatgac gactggtgta
     2161 tcgcccaggc cgcgaagaag ctgggacgga tggatgtcta tgccacctac agcaagcggg
     2221 cggagaacta taagaatgta tgggaccctt ctatcgggta tatgcgaccg aggctggccg
     2281 acggcagttt ccggaaggac ttcgatgtcc tcagcaccaa tggccagggt tttatcgagg
     2341 gcaatgcctg gaattacagc ctctatgtgc cccatgatcc ccgggcattg ataaaccttt
     2401 acggagggaa caagtctttt gtgcggcatc tggactcgct ttttaccatg gagctgccgg
     2461 acaggttctt tgccgagacg gaggatatca cgagggatgg cattatcggc aattatgtgc
     2521 atggcaacga gccatcgcac catgtggcct acctgtataa ctggacggac cagccctgga
     2581 agacgcagga gcgggtgcgt atgatcctga agaagcagta cgcgccggcg cccgatggac
     2641 tgggtggtaa cgacgattgc ggccagatga gtgcctggta tatttttacg gcgctgggat
     2701 tttaccccgt tgccccgggc tcggatgtct attcgcttgg cagcccggcg gtaaatgggg
     2761 cggtgatccg gttggacaat ggcaagacct ttaccataga ggctgataat cagggagata
     2821 aaaatgtcta tgtaaaaaaa gtaacgctaa atggtcagcc cctgaccggg catacgatta
     2881 cccatgagga gattatgaat gggggaaaac tggtattctt tatgagtgca aagcattaga
     2941 ccgggataga tgtctttttg ggcttgcaga catcgatttt ttttcatata ttagatatga
     3001 ggaagttgat ttttgtcaaa ttaaaatccc gtcatatgaa aaaaaccacc cactcgatgc
     3061 atgttggcga agccgttaca accattctgc tggctgtcgc cttccttggt ctggttatgt
     3121 ttttatctta tttgttcatt accggcaaca gctaagggcc gatcctgacc catacattac
     3181 gatgccggct cgcaagggcc ggcattttca atttatttac tgtacggaat aggccgtggt
     3241 gaattttccg ttggtgatga ccacgctgtc ggtggaggga ttgagatcgt tgagcagggt
     3301 gccgctgaaa gtcccgctga gtttgtgatt gacggtatcg aagaggctga tgttgaagat
     3361 ggcatggcca ttgccggcaa aggcgtcata ggacttgctg ccgctgcggg tatagtagga
     3421 caggccgaag ctggtgttat ccgaggaacg ggtagtatcg acggtaagcg gatagggtaa
     3481 gctcaactgc aggatggtcg agtcctttcc ctggggaacg aggccgatga cggtcatggt
     3541 ggagagggag gaataatagg cgccgaaggt ttggttggcc gtgaaagccg aagatccgac
     3601 ggtggcggac agggtcccct gggagttggt gctgctgttg ttcttactgc agttggtgag
     3661 gaggagggaa gtaagcaggg cgaggacggc gtatcgcatc atttttgggg gtgaagttaa
     3721 aaaaaaagag gccgtcgtac tgttaaaaat agacggcctc ttttttcatt atgtccggta
     3781 agcgcagctt accgggggcc ggctgacgcc ggcttaggaa agagatttgt gaaattctta
     3841 gggctgaacg gtatacgagg tgttgaactt accattggta accaggacgc tgtcagtgcc
     3901 gctgatccca ttgtagaggg tcgcggtgaa gttgcccgcg atcttgtggt tcacggtatc
     3961 cagcgaagta accgtcaggg ccgagtggcc aacaccgtag ccggcgaaat aatccttgtt
     4021 ggtagcaaaa tcataataat cgatgctaac cgaggaattg ccggacacca ccgtgttgac
     4081 ggtaaatggc ggggcgataa taagggccag tgtggtcgtg tcgtgcgcgc tcatggtata
     4141 gccgccaatt tcataaatgg aactgtcggt ggaataccag gcggtagtgc cgctggtatt
     4201 gacgccggtc gtaccgatgg tggcagaaag gctgttcgac ggagtgttgt tgcttttttt
     4261 acaactggtg atcaggacaa aaagggttac tgcggaaagg gaaagacgtg caaggtgttt
     4321 gatttgcata gtcaggtttt ttggtttttt atataggaat aaaagatacc cgcagaaaac
     4381 gcggaggaga aggctttatt gtcgcggcgg caaccggaat ttgaccgata tttgccgctc
     4441 ccatgctatc catcggaaag gtcatattac ccgattctcc cttgttgctg gcgcccatgg
     4501 aa
//
LOCUS       DATGFG010000955         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_965374, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000955 DATGFG010000000
VERSION     DATGFG010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965374"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VKQ52_18025"
     CDS             complement(<1..590)
                     /locus_tag="VKQ52_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89162.1"
                     /translation="MKIVSLFILLTVLLLGRTLAQGEIKEQLVVPLSEPGKPFKVVAN
                     VMHGSLKVIGYEGKDVVIDVTGEPGRKHESSGGSGGMRRIGGGESGDLTAEEDKNTVT
                     VNAGMSHFSLIEIKIPQNSATINLGAINDGNIVVDNVSGQLEVTNVNGAVTLSNISGS
                     VVANTVNGDVVVGFKSVDPKAPMAFSTLNGKIDVTFP"
     gene            complement(611..1489)
                     /locus_tag="VKQ52_18030"
     CDS             complement(611..1489)
                     /locus_tag="VKQ52_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89163.1"
                     /translation="MKKIIRPLLTGLAMLCLVSLAPAGARAQEFKEHINKEFAAPKPS
                     TEVLAIYNLNGPIKVEGYAGDKVVVDIDKTISAEGKDDLELGKRELQLGYDQHGDSVI
                     IYIAEPFDTRPHEWHRDQRWDRQDHNYDFNLSFVVRVPFGMNLHVSTVNHGEIVVQDV
                     TGDLSVHNVNGPVSIKNAKGATVARTINGNLTVNYVSAPRGPSEYYTLNGTLEVTYPA
                     DLSAVCQFKSMNGSFYTDFPDAEALPVTVTKNEQHRTSGTVYRLNVAKRVQIGSGGSL
                     FKFETMNGNIYVKKQS"
     gene            complement(1502..>2363)
                     /locus_tag="VKQ52_18035"
     CDS             complement(1502..>2363)
                     /locus_tag="VKQ52_18035"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HLZ89164.1"
                     /translation="LTRFLHEELSGEEREQVEAHLTECAECRREWESLRRIWGMLDEV
                     PVPEPAAGAQLRFDAMLDGYKESLGQSAGMSAGASAGMEGRGSLIGMEGRGGGSLWAL
                     LRRLFAGHPAFALGYSLLMVVAGLGGGYLLYRSPSQTAEPGDKKELVALTAQVAEMRE
                     MMMKTLLQNPSASERMRGVSYTSEIQAVNKDVIEALLSTLNNDPNTNVRLMTLEALTH
                     YANNPLVREGLVQSILQQESPLVQAALADVMLRLQEKRAIRPLKKLLQHKDLNEMVRT
                     RIEETISRLS"
BASE COUNT          491 a          746 c          587 g          539 t
ORIGIN      
        1 gggaaggtaa catcgatctt cccgttcaac gtagagaagg ccatcggcgc ctttggatcg
       61 acactcttga aacccaccac cacatcacca ttcaccgtat tggccaccac cgacccggag
      121 atgttggaaa gcgtgaccgc accatttaca ttggtgacct ccagttgacc actgacattg
      181 tcgacgacga tattgccgtc attgatagcg ccgagattga tagtagcact gttctgcgga
      241 atttttatct cgatcagcga aaaatggctc atcccggcgt tgaccgtaac ggtgttcttg
      301 tcctcttccg ccgtgagatc cccgctctcg ccgccgccga tccgccgcat cccgccactg
      361 ccgcccgaag attcatgttt ccggcccggc tcacccgtca catcgatgac gacatccttg
      421 ccttcatatc cgatcacctt caacgaacca tgcatcacat tcgcaacgac cttgaaaggc
      481 ttccccggct cactcagggg aacgaccaac tgctccttta tttccccctg cgcgagggtc
      541 cggccgagga gaagaaccgt caataagata aacagggaaa ctattttcat tgtcttcata
      601 ttttaatgat ttaagattgc ttctttacgt aaatattgcc gttcatggtt tcgaacttga
      661 aaagactgcc gccgctcccg atctgcaccc gcttggcgac attcagccgg tataccgtcc
      721 cggatgtgcg gtgttgctca tttttcgtga ccgtcaccgg cagagcttcg gcatcgggga
      781 agtcggtata gaaggagcca ttcatgcttt tgaattgaca caccgcagaa agatccgccg
      841 ggtacgtgac ctcgagcgtc ccattcagcg tgtaatattc cgaaggaccc cggggcgccg
      901 aaacatagtt gacggtgagg ttgccattga tcgtccgggc caccgtagcc cccttcgcat
      961 ttttgatcga caccggcccg ttcacattgt ggacgctcag gtcgcccgtc acatcctgta
     1021 ccacgatctc tccatgattg acggtggaaa catgcaggtt catcccaaac ggcacccgaa
     1081 cgacaaagga tagattgaag tcatagttat ggtcctgccg gtcccaccgt tggtcccggt
     1141 gccactcgtg cggccgggtg tcgaagggtt cggcgatata aataattacg ctgtcgccat
     1201 gctggtcgta ccccagctgt aattcccgct tgccaagctc caggtcatcc ttgccctccg
     1261 cggagatggt tttatcgatg tcgacaacca ccttgtcacc ggcgtagccc tctactttta
     1321 tcggtccgtt aagattatag atcgccagca cttccgtcga aggttttggg gccgcgaact
     1381 ccttgttgat atgctccttg aattcctggg cccgggcgcc ggccggggca agagaaacaa
     1441 gacaaagcat cgccagaccc gtcagcaacg ggcgaatgat ctttttcata tttgctaggt
     1501 tttaacttaa tcgggaaatc gtctcttcga tcctggtccg caccatctcg ttgaggtctt
     1561 tgtgttgtaa taatttcttc aacggccgga tggctctttt ctcctgcagc cggagcatga
     1621 catcggccag cgccgcctgc accagcggcg attcctgctg gaggatcgat tgcacaaggc
     1681 cctccctgac aaggggatta ttggcataat gggtcagggc ctcgagcgtc atgagccgga
     1741 cattggtatt cgggtcattg ttaagcgtgc tgagcaaggc ctcgatcacg tctttgttca
     1801 ctgcctggat ctcgctggta tagctgaccc cgcgcatccg ttccgaagcg gaggggttct
     1861 gcaaaagggt cttcatcatc atctcccgca tttccgccac ctgtgccgtc agcgccacca
     1921 actccttctt atcgccaggt tccgcggtct gcgatggcga ccggtaaagc aggtagccac
     1981 cccccaaccc cgccaccacc atcaggaggc tataccccag cgcaaaggcg gggtgaccgg
     2041 caaaaagccg ccgcaacaac gcccaaaggc tgccaccgcc acgcccctcc atcccgatca
     2101 aactaccgcg tccttccatt ccggctgatg cgccggccga catcccggcc gactgcccga
     2161 gcgattcttt atagccgtcc agcatggcgt cgaatcgcag ctgtgcgcct gcggccggct
     2221 cgggaacagg aacctcatcc agcatacccc agatccgccg aagggactcc cactcgcgcc
     2281 ggcattccgc acattcggtc aaatgcgctt ccacctgctc ccgttcctcc ccggacagct
     2341 cctcatgcag gaaccgggtc aac
//
LOCUS       DATGFG010000956         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_755878, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000956 DATGFG010000000
VERSION     DATGFG010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755878"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1142
                     /locus_tag="VKQ52_18040"
     CDS             132..1142
                     /locus_tag="VKQ52_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLZ89165.1"
                     /translation="MKTVFGEIRKLESVPSFSKHDRLVQGVINAIDEKLLSPDDLLPS
                     LNEMIRELKFSRDTVVKGYQELINRGLVGSTRGRGYYVTNGNTGQTLLVALMMYNMDT
                     FEEQFYRNFRAQLGPNVHLNVFFHHGNIDVFDTILNQIKGKYGMYVIAPIPHPRTKAL
                     LEAIPRHRFLMVDRYEPIQGEFNHVTQEFARSSYRIFMELAPVIRTFDEMIFYHSEDS
                     LDPKEIVGAFRKFLKDSKIKGRILPEFKPGTMEKGKVYFTLDNYALFAMLKECKAKKL
                     VPGKDLGILSHNDEPAKELIGITTYSADFSLMGKMAGEFVMRREMVRETMPMVLCRRN
                     TL"
     gene            1143..>2085
                     /locus_tag="VKQ52_18045"
     CDS             1143..>2085
                     /locus_tag="VKQ52_18045"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89166.1"
                     /translation="MRNSVTIGSFLLFTGIIAFVAWRQARKTRIETLSALFLAGRSLG
                     FLVVGGGLLFANINTATIVGENELVYTNNMSVMAWGVTSVLAMLVVSEFFMPVYLRTG
                     IATTPEYLARRYDRATGKLVSIIFLVSYVVNLLPTVLYSGAVAFNGLFHIADHWGIGY
                     WPTIWILVWIMGATGCLYSVLGGLRAIAVSDTLLGMGIFAGGILLPFFALRYLGHGDL
                     LTGLHTVLANHREHLNSIGSPADPIPFPTLFTGMLLVNLYYWGTEQYIVQQVLASRDL
                     ATCQQGIAVACLGKLVSPLLLNIPGLIAVHVFTRLHNT"
BASE COUNT          447 a          560 c          603 g          475 t
ORIGIN      
        1 gtccgagctg caggccgacc cgcagctgac ccaaaatccg ggctacaatt gatcctgcca
       61 tggccgcccg tttttggaaa cccgggcggc catgcttatc ttgatgtctc ttggttcaaa
      121 ctaacaggaa tatgaaaacg gtttttggtg aaataagaaa gctggagtcg gtgccttctt
      181 tttcaaagca tgatcgcctc gtgcagggcg tcatcaatgc catcgatgaa aagctcctgt
      241 cgccggatga tctgttgccg tcactgaacg aaatgatccg ggagctgaag ttctcacgcg
      301 atacggtggt gaagggctac caggagctca taaaccgggg gttggtaggc tccacccgcg
      361 gaagggggta ttacgtcacc aacggcaaca ccgggcagac cctgctggtg gcgttgatga
      421 tgtacaatat ggataccttc gaggaacaat tctaccgaaa tttccgtgcc cagctggggc
      481 caaacgtcca cctgaatgtc ttctttcacc atggcaatat cgatgtgttc gacacgatcc
      541 tgaaccagat caaggggaag tacggtatgt acgtcatcgc acccatcccg catccgcgga
      601 caaaagcgct gctggaagcg atcccgcgcc accggttcct gatggtcgac cgctatgagc
      661 ccatccaagg ggaatttaac cacgtcacac aggagtttgc ccgctcgtcc taccgcatat
      721 tcatggagct ggcgcccgtg atccggacat tcgacgaaat gatcttttac cattcggagg
      781 actcgttgga tccgaaagag atcgtgggcg cattcaggaa attcctgaag gatagcaaaa
      841 taaaaggcag gatactcccg gagttcaaac ccggaacgat ggagaaagga aaggtttatt
      901 tcaccttgga caactatgcg ctgtttgcga tgctcaagga atgcaaggca aagaaattag
      961 tgccgggcaa agacctcggg atcctgtcgc ataacgatga gccggccaag gagctgatcg
     1021 gcatcaccac ctattccgcc gacttttcgc tgatggggaa gatggcgggt gaattcgtga
     1081 tgcgccggga gatggtccgg gagacgatgc ccatggtgct gtgtcggaga aatacattat
     1141 aggtgagaaa tagcgttacg ataggcagct ttcttttgtt cacggggatc atcgcctttg
     1201 tggcctggcg ccaggcgcgg aagacccgga tcgaaacatt gagtgcgctt tttctggcgg
     1261 ggcgaagcct gggtttcctg gtcgtcggcg gcggattgct tttcgctaat atcaacacgg
     1321 cgaccatcgt cggggagaac gagcttgtct acacgaataa catgagcgtg atggcctggg
     1381 gagtgacctc ggtcctggcg atgctggtcg tctcggaatt ctttatgccg gtctatctgc
     1441 ggacaggcat cgcaaccacg ccggaatacc tggccagacg ctacgaccgt gccacgggca
     1501 aactggtttc gatcattttc ctggtgagct atgtcgtgaa cctgctcccc accgtactgt
     1561 atagcggtgc ggtagccttc aatgggttgt tccatatcgc ggaccactgg ggcatcggat
     1621 attggccgac gatctggatc ctcgtctgga tcatgggcgc cacgggttgt ttgtatagcg
     1681 tgctgggagg actgcgggcc atcgccgtct ccgacaccct gctggggatg ggcatcttcg
     1741 cgggcggtat cctgcttccc ttttttgcgc ttcgttacct ggggcacggc gacctgttga
     1801 cgggattgca taccgtactg gccaatcacc gggagcatct gaatagcatc ggctcaccgg
     1861 ctgacccgat ccccttcccg acacttttta cggggatgct cctggtgaac ctctattatt
     1921 ggggaaccga acaatatatc gtgcaacagg tgctggcctc ccgcgacctg gccacctgtc
     1981 agcaggggat tgccgtggcc tgcctgggta agctggtgtc ccccctgctg ctcaatatcc
     2041 ccgggttgat cgccgtgcat gtcttcacgc gcctgcacaa cacgg
//
LOCUS       DATGFG010000957         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_215650, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000957 DATGFG010000000
VERSION     DATGFG010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215650"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1311)
                     /locus_tag="VKQ52_18050"
     CDS             complement(28..1311)
                     /locus_tag="VKQ52_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89167.1"
                     /translation="MKRITFILTILLVKNAAANNTPVHNAFTTNSHNTRASRGVGARP
                     LGDPGIPGLSATAGVGMPSNFVRAASPRSSSFLTFAGSVTPRMVNFNHPKSPARESAA
                     QKGTFRIGQPSRQQTIPDASIIDTTWPSADLSSGPLHARFYLPNAATGYYRSTRFDWS
                     GVMPSLEYKGHTFFGQWFPKYDPTLNDAIMGPVESFTPIGYDQAQPGGPFLQLGVGIL
                     TRPDTSRYTPFKYYPIQNPGYWNITKIAGAIAFRQDLNAAGYSYVYIKTITFTPGQPQ
                     MVITHTLKNTGSQPLESDVYDHNFFVIDTQTVAPGRVLKLPWTPTASDARGLGELADI
                     RGDSIVFLQTFAPRQSVYAILHGYGDKPADYDIRIEEHRTGAGVRIRCDRPLAKLAFW
                     ASIKTACPEPFIHIKAAPGETITWTLTYDFYVLTP"
     gene            complement(1316..2566)
                     /locus_tag="VKQ52_18055"
     CDS             complement(1316..2566)
                     /locus_tag="VKQ52_18055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HLZ89168.1"
                     /translation="MSIRFRLTVMNFLEFFVWGAWLISLGGYMFNVLHFNGVQVGSIY
                     GTMGIASIFTPALFGIVADRWLNAERVLGLCHFIGAVMLLWASTLHDYGTFYIAMLLN
                     SFFFMPTIALNNTVSYIILERKNFNIIRDFPPIRVWGTVGFVCAMWLVDLTHCSLSPL
                     QLYISAGAGIVLGLYAFTMPPCTPVPNTTKRSFASSLGLDAFVLFRRKKMLIFFIYAM
                     LLGFALQISNAFASGFLNDFSGDPRYADSFGVRHSTLLISISQISETLFILTIPFFLR
                     RFGIKQVIIMSIFAWVFRFGLFGVGNPGSGLPLLVLSMVVYGMAFDFFNISGSLFVER
                     EADIKIRASAQGLFMLMTNGFGAWIGTYISGRVVDYFTVNGVKDWHNIWLSFSAYALV
                     LGITFPLVFRYKHDPTSVTPALQH"
     gene            2669..3844
                     /gene="mqnE"
                     /locus_tag="VKQ52_18060"
     CDS             2669..3844
                     /gene="mqnE"
                     /locus_tag="VKQ52_18060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HLZ89169.1"
                     /translation="MNTMTQGSDILLKTADKDLQRIGEKINAGERISPEEGLLLFEKG
                     SLPFLGSLANGVRERLHGNKTYFNRNFHIEPTNVCVFTCNFCSYSRVYAHRDEGWELS
                     QQQMLDMVKKYDGKPVTEVHIVGGVHPKMNMEFFMELLQKIKVHRPELHIKGFTAVEL
                     DYMFRKAKVSVEEGMRRMHAAGLDSMPGGGAEIFHPDVRSVICADKVDADGWLAIHAA
                     AHREGMHTNATMLYGHIETYAHRIDHMNRLRELQDQTRGFNTFIPLKFRNKGNDMSTI
                     PESSVVEDMKLYAVARLYMDNFPHLKAYWPMLGRQNAQLTLAFGVNDLDGTIDDTTKI
                     YSMAGSEEQTPSLSTTQLVSLIRQVGREPVERDTLYNEIRNYKDVDLDKMMQDPTLN"
     gene            complement(3871..>4483)
                     /locus_tag="VKQ52_18065"
     CDS             complement(3871..>4483)
                     /locus_tag="VKQ52_18065"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLZ89170.1"
                     /translation="DGWAYDIGYGGLDHVLSTGENVNIFVMDTEVYSNTGGQKSKSTP
                     IGASAKFSVNGKTTGKKDLALQAIAHGTAYVAQIAMGANDSHTLRTIIEAENFPGPSL
                     IIAYAHCIAHGIEMSEGAEHQDLAVRTGYWPLFHYNPLKPQGQRFVIDSKEPSLPLAD
                     FLYRENRFKVVVAKDPALAAKFLTEAEDATKERWSRLKTLTGL"
BASE COUNT          988 a          956 c         1554 g          985 t
ORIGIN      
        1 tatgctaata tatcgaattt tccggggcta tggtgttaac acatagaaat cgtaagtgag
       61 ggtccaggtg attgtctcgc cgggagcggc ttttatgtgg atgaagggtt cggggcaggc
      121 ggttttgatg gaggcccaga aggcgagttt tgccagtggc cggtcgcagc ggatgcggac
      181 gccggcgccg gtgcggtgtt cttcgatgcg gatatcgtag tcggcgggtt tgtcaccata
      241 tccgtggagg atggcataga cggattgtct tggggcgaag gtttggagga agacgatgga
      301 gtcgccgcgg atgtcggcga gctcgccgag gcctcttgcg tccgaggccg tgggggtcca
      361 ggggagtttg aggacgcggc cgggggcgac ggtttgggtg tcgatgacga agaagttgtg
      421 gtcatatacg tccgattcga ggggctggct accggtgttc ttcagcgtat gggtgatgac
      481 catctggggt tggccggggg tgaaggtgat ggtctttata tagacatagg agtagccggc
      541 ggcgttgagg tcctgacgga aggcgatggc gccggcaatt tttgtgatat tccagtagcc
      601 ggggttttgg atcgggtagt atttgaaggg ggtgtagcgg gaggtgtcgg gccgggttag
      661 gatgccgacg cccagttgga gaaaggggcc gccgggttgg gcctggtcat agccgatggg
      721 ggtgaaggat tcgacgggcc ccatgatggc gtcgttgagg gtggggtcgt attttgggaa
      781 ccattgcccg aagaaggtat ggcccttgta ttcaagggag ggcatgacgc cggaccagtc
      841 gaagcgggtg gagcggtagt agccggtggc ggcgttgggg aggtagaagc gggcgtggag
      901 ggggccggag gagaggtcgg cggaaggcca ggtggtgtct attattgagg cgtcaggtat
      961 ggtttgctgc ctgctcggct ggccgatgcg gaatgtcccc ttctgcgctg cggattccct
     1021 cgctgggctt tttggatggt tgaagttaac catccgaggg gtaaccgagc ctgcgaaggt
     1081 taggaacgag gaagagcgtg gcgaagcagc tctgacgaag ttcgagggca tcccgacacc
     1141 ggccgtcgcg gagaggccag ggataccggg atcgccaaga gggcgcgcac ctacgccacg
     1201 actggccctg gtgttgtggg agtttgttgt gaaggcgttg tggacaggtg tgttgtttgc
     1261 ggctgcgttt ttgacaagga ggatggtgag gatgaaggtt attcttttca tatcgctagt
     1321 gttgtagtgc cggcgtgacg gacgtggggt cgtgcttata gcggaaaacc agggggaagg
     1381 tgatccccag gaccagggcg taagcagaga aggaaagcca gatgttgtgc cagtccttta
     1441 ccccgttgac cgtgaaataa tcgacgacgc ggccgctgat ataggtgccg atccaagctc
     1501 cgaatccatt tgtcatgagc atgaagagcc cctgggcgct ggccctgatc tttatatcgg
     1561 cttcgcgttc cacgaagagg gagccggaga tattgaaaaa gtcgaaggcc ataccataga
     1621 cgaccatgga aaggacgagg aggggcaggc cgctgccggg gttgccgacg ccgaagaggc
     1681 cgaagcggaa gacccaggcg aagatgctca taatgatgac ctgcttgatg ccgaagcgac
     1741 ggaggaagaa gggaatggtc aggataaaca gggtttcgga tatctgggag atggaaatga
     1801 gcagggtgga gtggcggacg ccgaaggagt cggcgtagcg ggggtcgccg gagaagtcgt
     1861 tgaggaagcc gctggcgaag gcgttgctga tctggagggc gaatccgagg agcatagcgt
     1921 aaataaagaa tatgagcatt tttttccggc ggaagagcac gaaggcgtcg aggccgaggg
     1981 aggaggcgaa gctgcgtttc gtcgtattgg gtaccggggt gcagggcggc atggtgaagg
     2041 catagaggcc caggacgatg cccgctccgg cactgatata gagctgtagg ggactgaggc
     2101 tgcaatgcgt gagatcgacc agccacatgg cgcagacgaa acccaccgtt ccccagacgc
     2161 ggatgggggg aaagtcccta atgatgttga aattctttct ttcgaggata atataggaaa
     2221 cggtgttgtt gagcgcaatg gtgggcatga agaagaagga gttgagcaac atcgcgatat
     2281 agaatgtccc atagtcgtga agggtggaag cccagaggag cataacggcg ccgatgaagt
     2341 gacagaggcc gaggacgcgt tcggcgttga gccagcggtc ggcgacgata ccgaagagcg
     2401 cgggagtgaa gatggaggcg atacccatcg ttccatagat gctacccacc tgcaccccat
     2461 tgaaatggag gacgttgaac atatagccgc ccagagagat gagccaggcg ccccagacga
     2521 agaattcgag gaagttcatt acagtgagcc ggaatcttat actcatgcgt tattttggtg
     2581 atatttggtt ctgatagtac aaacagggaa gatacggcat ttatggatgc tgtacaatcc
     2641 ttaattttgc cttttaaata ccggaattat gaataccatg acacaaggat cggatatttt
     2701 gttgaagacg gcggataagg atttgcagcg catcggggag aagataaatg cgggtgagcg
     2761 gatcagcccg gaggagggtc ttttgctttt tgagaagggg tccctaccct ttcttggttc
     2821 gctggccaat ggagtgcggg agcggctgca cggcaacaag acctatttca accggaattt
     2881 ccatatcgag ccgaccaatg tgtgtgtgtt cacctgtaat ttctgctctt attcaagggt
     2941 gtatgcgcac cgcgatgaag gctgggaact gtcgcagcag cagatgctgg atatggttaa
     3001 gaaatacgac ggcaagccgg tgacggaagt ccatatcgtc ggcggggtgc atcctaagat
     3061 gaatatggag ttctttatgg agttgctgca gaagatcaag gtgcatcgtc cggagttgca
     3121 tatcaagggt tttacggcgg tggagctgga ttatatgttc cggaaggcga aggtgtcggt
     3181 ggaggaaggg atgcggcgga tgcatgcggc gggcctggat tcgatgccgg gcggcggagc
     3241 ggagatcttc cacccggatg tgcggagcgt gatctgtgcc gataaggtgg atgccgacgg
     3301 ctggctggcc attcatgccg ccgcgcatcg cgaggggatg catacgaatg cgacgatgtt
     3361 gtatgggcat atcgagacgt atgcgcatcg catcgatcat atgaaccggc ttcgggaatt
     3421 gcaggaccag accaggggtt ttaacacgtt catcccgctg aagtttcgca ataagggtaa
     3481 tgatatgagt actattccgg agtcgtcggt ggtggaggat atgaaattat atgcggtggc
     3541 gcggttgtat atggataatt tcccgcatct gaaggcgtac tggccgatgc tggggcggca
     3601 gaatgcgcag ctgacgctgg cgtttggcgt gaatgacctg gatgggacga tcgatgatac
     3661 gacgaagatc tattcgatgg cgggcagcga ggagcagacg ccgagtttgt cgacgacgca
     3721 gctggtgtcg ttgatccggc aggtgggcag ggagccggtg gagagggaca cgttgtacaa
     3781 tgagatacgg aattataagg atgtggatct ggataagatg atgcaggatc ctacactgaa
     3841 ttaaacgacc tgttaaatgg cggggactgc ctacaagccc gtcagggtct tcagtcttga
     3901 ccatctttct tttgttgcgt cctctgcctc ggtgaggaat ttcgcagcca acgcggggtc
     3961 ctttgccacc acaaccttga aacggttttc ccggtacaag aagtcggcaa gcggaagcga
     4021 aggttctttt gagtcgatca caaaccgttg tccctgaggt ttcaggggat tatagtggaa
     4081 caagggccaa tacccggtcc tgacggccag gtcctggtgc tccgcaccct cactcatttc
     4141 gatgccatgg gctatacaat gtgcataggc gataatgagc gaggggcccg ggaaattttc
     4201 cgcctcgatg atggttcgaa gggtatggga gtcgttggcc cccatcgcga tctgggctac
     4261 ataggcagtc ccatgagcta tggcctgcag ggcgaggtct ttcttgccgg ttgtcttccc
     4321 gttcacggag aattttgcac ttgccccaat gggcgtggat ttcgattttt gaccgcccgt
     4381 gttggaatag acctccgtat ccatgacgaa tatattcaca ttttcgcccg ttgaaagcac
     4441 gtggtccaac cccccgtaac cgatatcata agcccagccg tcg
//
LOCUS       DATGFG010000958         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_520807, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000958 DATGFG010000000
VERSION     DATGFG010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520807"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VKQ52_18070"
     CDS             <1..492
                     /locus_tag="VKQ52_18070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLZ89171.1"
                     /translation="FLRELSRQMIDLPAPSFTLQDLDGHTVSLDSLRGKTVVVDFWAT
                     WCGPCKASFPAMQMAVNKYRDSNDVRFLFIHTWEHDDTATRAARQFIVAKQYPFQVLM
                     DLKDKTTGSNRVVEDYKVSGIPTKFVIDRNGRIRFRLTGFEGGNDAAVDELTAMITLA
                     GGK"
     gene            complement(521..1414)
                     /locus_tag="VKQ52_18075"
     CDS             complement(521..1414)
                     /locus_tag="VKQ52_18075"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ89172.1"
                     /translation="MQPIRHRLFLLASAALSLTGCVSAGAYKSLQQQAQKSDSLYTWS
                     QQSLKTCQDANDALARKTASLQNETNDMKLQLTAAQENNGQLRKQVQDMAAISSAQAE
                     SIKRSIDNIGAKDQYIRDLRSAIGHRDSVNFAVLMDMKSMMGGFGDQDVNIRLEKGIL
                     YVDLSEKLLFGGDSSSYMVNDKAKGLLARLAKVMTAEPDVEFTVEAHTESVVHPQDIL
                     VDDWDLSAKRATSVVRILQNEYNISPTRMTACGRSEYTGMAPSDTPEGRAANRRTRIT
                     ILPQLDRLLRLLERGQGSTDM"
     gene            complement(1462..2013)
                     /locus_tag="VKQ52_18080"
     CDS             complement(1462..2013)
                     /locus_tag="VKQ52_18080"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLZ89173.1"
                     /translation="MRKLVASAWITLDNVFDSSLMPQWFFPFDSKSRQQAIRDSVMGS
                     DVILMGRSTYEMLMPYWSKLHDGEMDGVAGKLNHSPKYVVSANLSAAPWENSTIIRDN
                     VAETVARLKEEPGSEIRIEGSGMLANSLMKAGLVDEYRLLIHPCVVGSGKRYFDAGMP
                     PKGLRLASCAPLDHGVLRVVYGV"
BASE COUNT          455 a          678 c          533 g          446 t
ORIGIN      
        1 ttcctcaggg aactgtcaag gcagatgatc gatctgcccg ccccctcctt taccctgcag
       61 gacctcgatg gccatacggt cagcctcgat tctttgcggg gaaagaccgt ggtggtcgat
      121 ttctgggcca cctggtgcgg cccctgcaag gcctcctttc cggcgatgca gatggctgtc
      181 aacaaataca gggatagcaa tgatgtgaga ttcctgttca tccacacctg ggagcatgat
      241 gacaccgcca cccgggcggc ccggcaattc atcgtcgcga aacaatatcc cttccaggtg
      301 ctcatggacc taaaggataa gacgacaggc agcaataggg tagtggagga ctacaaggtc
      361 tccggtatcc ccaccaaatt cgtcatcgac aggaatggcc gcatccgctt ccggctcact
      421 ggcttcgaag gtggtaatga tgcggcagtc gatgaactca cggcaatgat cacgctggca
      481 ggagggaaat aaccccctgc cggctccggc tcacgtctgc ttacatatca gtcgatccct
      541 gcccgcgttc cagcagcctc aacagtctgt ccagttgagg aaggatcgtg atcctcgtcc
      601 ggcgattggc cgccctgcct tccggggtat cgctgggcgc catccctgta tactcgctgc
      661 ggccacaggc cgtcatccgg gtaggggaga tattgtattc gttttgcagg atgcggacca
      721 ccgaggtcgc gcgcttggcg ctgagatccc agtcatctac caatatatcc tgcggatgca
      781 ccacgctctc cgtatgcgcc tcgaccgtaa actccacatc gggctcggcg gtcatcacct
      841 tcgccagcct ggccagcagc ccctttgcct tatcattcac catatagctg ctgctgtcgc
      901 cgccgaacaa cagcttctcg gaaagatcga catacaggat acctttctcg agcctgatat
      961 tgacatcctg gtccccaaaa ccacccatca tgctcttcat gtccatcagc acggcaaagt
     1021 tcaccgaatc ccggtgcccg atcgccgaac gcaggtcccg gatatactga tccttggccc
     1081 cgatattatc gatcgatcgc ttgatagact ccgcttgcgc ggaactgatc gccgccatat
     1141 cctgcacctg cttccgcaac tgcccattat tctcctgggc cgcggtcaat tgcagcttca
     1201 tgtcattggt ctcattctgc aacgacgccg tcttcctggc cagggcatcg ttggcatcct
     1261 ggcaggtttt cagcgattgc tgtgaccacg tataaaggga atcgcttttc tgcgcctgct
     1321 gctgcaggga tttataggcg ccggcgctga cacagccggt tagggatagc gcggcggaag
     1381 cgagaaggaa aagccggtga cggatgggtt gcataacaga ggggtttacc atgccctcaa
     1441 agataataaa tcccccggca ttcacacccc atacaccacc cgcagcaccc catggtccag
     1501 cggcgcacaa ctcgcgagcc gaaggccctt cggcggcatc cccgcatcga aataccgctt
     1561 cccgcttccg accacacagg gatggatcag caaccggtac tcatcgacaa gccccgcctt
     1621 catcagggaa ttggccagca tgccgctgcc ctcgatccgt atctcgctgc ccggctcctc
     1681 tttcaaccgc gcaaccgtct ctgcaacatt atcccggatg atcgtcgaat tctcccacgg
     1741 cgccgcggaa agattggccg acaccacata tttcggcgaa tggttcagct ttccggccac
     1801 tccgtccatt tccccgtcgt gcagcttcga ccagtagggc atcagcatct cataggtgct
     1861 gcggcccatc aggatgacat cggagcccat cacggagtcg cgtatcgcct gctgccggct
     1921 cttgctgtca aaaggaaaga accactgcgg catcagggaa gaatcaaaga cattatctaa
     1981 tgtgatccag gcagaggcta cgagtttacg catgttgtat cgtttttgta tcggcaagat
     2041 acacagcccc cccagccgaa aaatagaaca aacccgacat tctcacaccc cctccaaaaa
     2101 cgccgccgtc cg
//
LOCUS       DATGFG010000959         4938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1192117, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000959 DATGFG010000000
VERSION     DATGFG010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192117"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VKQ52_18085"
     CDS             complement(<1..955)
                     /locus_tag="VKQ52_18085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89174.1"
                     /translation="MLQHYWRITWRILTRNKIYTFINILGLALGICGCLVLFLITRYE
                     FSFDRDHPDQGLIYRVVGEKQSPEETEFLNSPYDDLAAFETQIPGFSATAGVHGCRGR
                     ISVPQTGRPPKSFDNSIPGSYMPTAMFTTPGYFDILRYVWLAGSAGSLSRPNNVVLTK
                     SRARLYFGDLPAEEMLGKLIIYDDSLFVHVSGIVKDWEGNTDLGYTDILSVTTATTSF
                     LKKDIPTADWSSLQPHRSMAFVKLSPGVRPEQVNARFAEYIRRHIQLRQAGTSLRMYL
                     QSLKDMHFTHEFHRGDDGDGWRKPYLPTLYVMMGVAVFILLI"
     gene            1098..3578
                     /locus_tag="VKQ52_18090"
     CDS             1098..3578
                     /locus_tag="VKQ52_18090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ89175.1"
                     /translation="MNKLYALLLTLASTASLFAQEKNPANDNNGRITGRIIDSLSHQA
                     LQYATITLFKQKEAKPAGGVMTGNKGTFNIDGLAPGSYTITVELIGYAPYTLGPVTID
                     KKSVNLGDITVGKKATDLQAVTVTAPRGLIENKIDKMVYNVEKDVTSLGGVATDVLKK
                     VPMVSVDVDGNVDIMGNSNILFLINGRPSSIFGNSLADALQTIPASQIKSIEVITSPG
                     AKYDAEGTGGIINIILKDNKLNGINGNISVTGGSRLQNGSLNLNARRGNFGMNAFFSG
                     NAQIPSTTHTSSLRRSFDSTGSDVLTQDGSSRFLRNGFESGLGFEWTPDKRNTFSANA
                     GYNNFGNHNQGSYQQDEQRFLPGANTPWFDLPSLIHSTSQFRAHSLDWNLRYKRTFAQ
                     EDRELDLSLDGSEGRNNSSYSQAQSKPTGDSTFAGSQANSRGQDHEMNVRLDYVQPFG
                     EKIRLETGGRLQIRRITSNSPVYTYNQALADYPYDSSQSNALTYDRHVYAGYASLSFP
                     LFNFLEVKGGLRYERTETDAGFSKAPNTTIPGYNTFAPTIIFSHSFSNDQTLKIGYAR
                     RIQRPDYRSLNPYVNASDPKNLTRGNPFLQPEIADNFDLTYSKSFDKGTAINVVLFYR
                     RSGHDIQPFIQYYSSFTLGDSTYTDVSVSTPMNVGSENNTGVNIYGSVPLGKKLNLRS
                     NISVFNRYITTGTLGGNAINSFNYRINLNASYQLSSTFVLEFFGNFNSARNEIQGRYP
                     SFTTYNFAARKQFWNKKASIAFTTTNPFNFYVNQATAVSGSNFTLNSFRRVPFQSFGL
                     NFTYKFGKLEFKKDKEENKEPAQPDIAP"
     gene            complement(3594..>4938)
                     /locus_tag="VKQ52_18095"
     CDS             complement(3594..>4938)
                     /locus_tag="VKQ52_18095"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HLZ89176.1"
                     /translation="NKGALVLNMLRVYLGDSAFFKGLELYLRVNAFRSAEVHQLRLAM
                     EEVSGRDLNWFFDQWFFGAGHPKVNIDYRYDEAGEVGVVISQGPGWSSGTAQGSGAAQ
                     GPGAAQGPGAAQGPGAAQGPVFTFPLVIDVYDSGGKTSYPVWVRKEKDTFTFPVAGRP
                     ELVNVDAEKMMIWEKTDHKTLVNFAFQYDHAGNFVDRREALDACFSQPDDPLALEVIR
                     KGLRDGYAGIRQYALLGLARGKTPGKGGVESAAGGVSTGKGGVESMVAGMAQGDPNSI
                     VRRYALRLLPDYGLKYRAVLLAELDDSSYSAAGEALNALGRIDSVAAYTAARAQFGQK
                     SRGTLAEEIVGTLAQYGTEEDFDSIMSRFAQLSFKPRISRKVAAYLGRVQSTARVEKG
                     VDLLVGLRRKLPEFYGPVINDNLRVVLRAKEEAGSTEQADYIRSRLPAGDGARGH"
BASE COUNT         1212 a         1709 c         1067 g          950 t
ORIGIN      
        1 caatgagcag gatgaaaacg gcgaccccca tcatcacata cagcgtcggc agataaggct
       61 ttcgccagcc atccccatca tcgccacggt gaaattcatg ggtaaaatgc atgtctttca
      121 gcgattgcag atacatcctc agggaagtac ccgcctgccg caactggata tgccgccgga
      181 tatattccgc gaaccgcgca ttcacctgct ccggcctcac cccgggggaa agttttacaa
      241 aagccatgct ccggtgcggt tgcaggcttg accagtccgc ggtcgggata tccttcttca
      301 aaaagctggt cgttgcggtt gtcaccgaca ggatatccgt atacccaaga tcggtattcc
      361 cctcccagtc cttcacgatc cccgatacat ggacaaaaag cgaatcgtca taaatgatca
      421 gctttccaag catctcctcc gcgggcagat ctccgaaata caatctggcc ctgctcttcg
      481 tcaacacgac attgttcggc cggctaagcg atcccgcaga acccgccagc cagacataac
      541 gcaggatgtc aaaataaccc ggcgtagtga acatggccgt aggcatataa ctccccggaa
      601 tactgttgtc aaaggacttc ggcggccggc cggtctgcgg aacactgatc ctgccgcgac
      661 aaccatgcac tccggccgta gcgctaaacc cgggtatctg cgtctcgaag gccgccagat
      721 catcataggg actgttcagg aattcggtct cctccgggga ctgcttctcg ccgacgacac
      781 gatagatcag accctgatcg gggtggtccc ggtcgaagga gaattcataa cgcgtgatca
      841 ggaaaagcac aagacaccca caaatgccaa gcgccagccc aaggatgtta ataaaagtgt
      901 aaatcttgtt gcgcgtgagg atacgccacg tgatccgcca gtaatgttga agcataagga
      961 aggtttttgc aataataaaa gacccggtac cgcccggtat taaaatgtga tgaaaacgcc
     1021 ggaataagtg tcgaacccgc tgccagcaca gattttgttc agaaagccgg cctaccttgc
     1081 tctcttttat tgtgaacatg aataaactgt acgctctcct gctaaccctg gcctctactg
     1141 cttctctctt cgcgcaagaa aagaaccctg caaacgataa caacggtcgc atcaccggcc
     1201 ggatcatcga ttccctttcg catcaggcgc tgcaatacgc gacgatcacc ctcttcaaac
     1261 aaaaagaagc aaaacccgcc ggtggtgtca tgaccggcaa caagggtacg ttcaatatcg
     1321 atggccttgc cccgggatcc tatacgatta ccgtcgagct catcggctat gccccctata
     1381 ctctcggccc ggttaccatt gacaaaaaga gcgtcaatct cggcgacatc accgtcggca
     1441 aaaaagccac cgacctccag gccgtcaccg tcacggcccc acgcggcctg atcgagaaca
     1501 agatcgacaa gatggtgtac aacgtcgaga aggacgtcac ctccctcggc ggcgtggcca
     1561 ccgatgtcct gaaaaaagtc ccgatggtct cggtcgatgt cgatgggaat gtcgacatca
     1621 tgggcaacag caatattcta tttctcatta acggccggcc ctccagtatc ttcggcaaca
     1681 gcctcgccga tgccctccag accatccccg ccagccagat aaaaagcatc gaggtcatca
     1741 ccagccccgg cgccaaatac gatgccgaag gcacgggcgg gatcatcaat attatcctga
     1801 aggacaacaa actcaacggt atcaacggca atatcagcgt caccggcggc tcccggctgc
     1861 agaacggctc cctgaacctc aacgcccgcc ggggtaactt cggcatgaat gccttcttca
     1921 gcggcaatgc ccagataccc tcgactacac atacctcctc cctgcgcagg tccttcgact
     1981 ccaccggcag cgatgtcctt acacaggacg gcagcagccg cttcctccgt aacggcttcg
     2041 aatccggcct cggattcgaa tggacccccg acaaacgcaa taccttctcc gcaaacgccg
     2101 gctataacaa cttcggtaac cataaccagg gctcctatca gcaggatgaa caacgattcc
     2161 tccccggcgc caataccccc tggttcgatc tgcccagcct catacactcg acaagccagt
     2221 tcagggctca ttccctcgac tggaacctgc gttataaaag aaccttcgcc caggaagatc
     2281 gggaactcga tctatccctc gacggcagcg agggccgcaa caacagttcc tattcccagg
     2341 cccagagcaa acccaccggt gactccacct tcgccggctc ccaggccaac agcaggggac
     2401 aggatcacga aatgaacgtc cggctcgact acgtccagcc ctttggcgaa aagatccgcc
     2461 tcgaaaccgg cggtcgcctc cagatccgcc gcatcacaag caattcgccg gtctacacct
     2521 ataaccaggc gctggccgac tatccctatg acagcagcca gtccaacgcc ctcacctacg
     2581 atcgccacgt ctatgccggc tatgcctcgc tctcctttcc actgtttaac ttccttgagg
     2641 taaagggcgg gctcagatat gaacgcactg aaacagacgc cggcttctca aaagcaccca
     2701 ataccacgat ccccggctat aatacctttg caccgacgat tatcttttca cactctttct
     2761 caaacgacca gacgctcaag atcggctacg cccgccgcat ccaacggccc gactaccgct
     2821 ccctgaaccc ctatgtgaac gccagcgatc cgaagaacct cacccgcggc aaccccttcc
     2881 tccaacccga gatcgccgac aacttcgatc tcacctacag caaatccttc gacaagggca
     2941 ccgccatcaa cgtggtactc ttctaccggc gctcgggtca cgatatccaa ccctttattc
     3001 aatattattc cagcttcacc ctcggcgact ccacctacac cgatgtctcc gtcagcactc
     3061 ccatgaacgt tggatcggag aacaacaccg gcgtcaatat ctacggttcc gttccgctgg
     3121 gaaaaaagct taatctgcgc agtaacatct ccgtattcaa ccgctatatc accaccggca
     3181 cgctgggcgg caatgcgata aacagcttta actatcgtat caacctcaac gcctcctatc
     3241 aattgagctc caccttcgtg ctggaattct tcggcaactt caactccgcc cgcaacgaga
     3301 tccagggccg ctatccttcg tttaccacgt acaacttcgc ggcccgcaaa caattctgga
     3361 ataaaaaggc aagcatcgcc ttcaccacta ccaacccctt caatttctat gtcaaccagg
     3421 ccacggcggt cagcggatcg aacttcacgc tcaacagctt ccgccgcgtg ccgttccaat
     3481 ccttcggcct gaactttacc tataaattcg ggaaactgga attcaaaaaa gacaaggaag
     3541 agaacaaaga gcccgcacaa cctgatatcg ccccctaaca acccatatgg cccctaatgg
     3601 ccccgggcgc catcgccggc cggcaaccgg ctccggatgt aatccgcctg ctccgtcgac
     3661 cccgcctcct cctttgccct cagcacgaca cgcaggttgt cgttgataac aggtccatag
     3721 aactccggta atttccggcg cagaccaaca agtagatcga cgcctttctc gaccctcgcc
     3781 gtactctgca cccgccccag gtaagcggca accttccgcg agatcctggg cttgaaagat
     3841 aattgggcaa accggctcat gatactatcg aaatcctcct ccgtcccata ctgcgccagc
     3901 gtcccaacga tctcttctgc cagcgttccc cggctcttct gcccaaactg cgcgcgggcc
     3961 gccgtatacg ccgcaacgga atcgatccgg ccaagcgcat tcaaagcctc acccgccgcg
     4021 ctatacgaag aatcatccag ctccgccagg agcaccgccc tatatttcaa accatagtcc
     4081 ggcaacaacc gcaatgcata ccggcggaca atggaattgg gatcaccctg cgccatcccg
     4141 gcaaccattg actccacacc tcccttcccc gttgacacgc cccccgccgc agactccaca
     4201 ccacccttcc ccggcgtctt ccccctcgcc agacccagca acgcatactg ccggatgccc
     4261 gcatacccat ccctcaaccc ttttcggatc acctccaacg caagcgggtc atcgggctga
     4321 ctaaaacaag catcgagcgc ctcccgccgg tccacaaaat ttccggcatg atcatattga
     4381 aaagcaaaat tgacaagcgt cttatgatcg gtcttctccc agatcatcat cttctccgca
     4441 tccacattca ccagctccgg ccttcctgca accgggaacg taaaggtatc cttctccttc
     4501 cgcacccaca cggggtaact cgtcttcccg ccgctgtcat acacatcgat gacaagcgga
     4561 aaagtaaaaa ccggtccctg cgctgctccc ggtccctgcg ctgctcccgg gccctgcgcc
     4621 gcccccggtc cctgcgccgc ccccgagccc tgcgccgtcc ccgaactcca ccccggcccc
     4681 tgcgaaataa ccacaccaac ctcccccgcc tcatcatatc gataatcgat attcaccttc
     4741 gggtgccccg cgccaaagaa ccactggtca aagaaccagt tcaggtcccg tccacttacc
     4801 tcctccatcg ccagccgcag ctgatggact tcggcggacc taaaagcatt gacccgcaaa
     4861 taaagctcca accccttgaa aaacgcgctg tccccaagat agacccgcag catattcagc
     4921 accaacgccc ccttgtta
//
LOCUS       DATGFG010000960         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_142982, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000960 DATGFG010000000
VERSION     DATGFG010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142982"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VKQ52_18100"
     CDS             <1..1109
                     /locus_tag="VKQ52_18100"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HLZ89177.1"
                     /translation="NQPSVKVSDVTQTRQAQTSPFRFYIDLSVPSQQTVTVGYSTSDG
                     TAKAGKDYTAASGTVMIPAGQTEVYVDVSVAGDSLRQTNLQFYLQLSNPVNSVLGTAK
                     AVGTILNTDLLYLPTDTTGYTTPTSYPGYHLVWSDEFNGNALSTQDWNYEQGNNNGWG
                     NHELENYTNRTQNVFVSAGHLVIEARQESFGGNNYTSGRLTTQNKQAVTYGRIDIRAK
                     LPVAKGMWPALWMLGSNITTVPWPGCGETDIMELVGSSPNQVTGSLHWQQGGGSEGTY
                     NNNYDLASGQDFSQQFHVFSLIWQNNSVQFLVDDHVYVSGGIANVTSGTYPFNSPFFF
                     IFNVAVGGDWPGAPDGTTSFPQRMFVDYVRVFQQ"
     gene            1127..2590
                     /locus_tag="VKQ52_18105"
     CDS             1127..2590
                     /locus_tag="VKQ52_18105"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HLZ89178.1"
                     /translation="MKTSKTVWFGILILSLGGLTACSKKSSNPAPPVTPPADTTKTPV
                     NSQVAFWLTNPDKTALFQQQNIALNFGSGLAVEPVITVDSTQAYQTIDGFGYALTGGS
                     AQLMYSLPAAQRVALEQELFGTDSTHIGVSYLRVSIGASDMSARVFSYDDNASPAQPD
                     TSLASFDLSDDKIYLVPLLKEILTINPTIKILGCPWSAPVWMKDNNNSKAGSLLSQYY
                     GVYANYFVKYVLAMKANGIAIDAVTPQNEPLNANNNPSMVMQDTAEERFVRDWLGPAF
                     KVAGIGTKIIVWDHNCDVPSYPLTILADGAAAQYVDGSAFHLYAGDISALSTVHNAYP
                     NKNVYFTEQYVGGPGNFASDLDWAIKNLIIGAPRNWSRNVLEWNLAADASYGPHTSGG
                     CGVCQGAFTIDGTTVGRNTSYYIIAHASKFVRPGSVRISSNLTGNLQNVAFLTPGGKK
                     VLIVLNDNAAAQTFTVQFNGKNFSTTLNGNAVGTYVW"
     gene            2636..3808
                     /locus_tag="VKQ52_18110"
     CDS             2636..3808
                     /locus_tag="VKQ52_18110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HLZ89179.1"
                     /translation="MTKVYLKKKISRRVENGHPWIFGNEVGTVEGDSAGGSIVDVFTH
                     DKKFLGRGYINLQSQISVRLLTRDKAETIDDAFFYSRLSEAWDYRRRLGYTENCRLVF
                     GEADYLPALIIDKFNDYFVLQTLALGMDRWKPAIVDALQRIFQPKGIYERNDVPVREL
                     EGLPQQKGFLSAPFDTQIVIRENGLKLHVDIENGQKTGYFLDQHDNRRAIQHIVKGAD
                     VLGAFCYTGSFETHAGFYGAKSVLGLDISENAVVQARRNAELNGLGEVCRYEAVNAFD
                     ALKQWSKDGRQYDVVMLDPPAFTKSRETIQKAITGYKEINLRGMKLVRKGGFLVTSSC
                     TNLVQPELFLETIQLAAKDARRRLRQVTFQAQASDHPIIGGWENTNYLKFLIVQVL"
     gene            complement(3813..4844)
                     /locus_tag="VKQ52_18115"
     CDS             complement(3813..4844)
                     /locus_tag="VKQ52_18115"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HLZ89180.1"
                     /translation="MKRNLQHIDEIRFLVIHLVLINHWNINLYLANAPYDHTIFDLWF
                     DLTSPCLAVLSGYLFFYRTQEDFHYLKKLKTRFHSLVVPYLFWTLSFFVVYIVVKDVY
                     TRLFHSTFWYAPEESLTFKNVLLNIINPPLVNFWYLQNLVLILPFNFIIYYLLKNKYV
                     FFAFFLLVLACYSFNLADLYFQSRFLPYYLMGCYFGYNEKFLPRIPVSKLATLILIPV
                     LTWVALSTSNWKDNLFPVLEIKLCVVFFFLLSVFNILDSNQNSLVFKYLNKYKPYSFF
                     LFAINMFLFSFVQRGLLKLGAERYLHYHAFLFLFLAGSVVVVIILAFAIAFLLKRYCT
                     KFYYTITGR"
BASE COUNT         1155 a         1049 c         1556 g         1114 t
ORIGIN      
        1 gtaatcagcc ttcggtgaag gtgagtgatg tgacgcagac gcggcaggcg cagacctcgc
       61 cgttccggtt ctatatcgat ctcagcgtcc ccagtcagca gactgttacg gttggctatt
      121 cgacttccga cgggacggca aaggcgggga aggattatac ggccgcttct ggtacggtga
      181 tgatacctgc cggtcagacc gaggtatatg tcgatgtgtc cgtcgcggga gacagccttc
      241 ggcagaccaa cctgcaattc tatctgcaat tgagtaatcc tgtgaatagt gtgctgggga
      301 cggccaaggc ggtggggacg atattgaata cggacctgtt gtatctgccg acggatacga
      361 cgggctatac caccccgacg agttaccccg ggtatcatct tgtgtggagt gatgaattta
      421 acggcaatgc actcagtacg caggattgga attacgagca gggcaataac aatggctggg
      481 ggaatcatga gttggagaac tataccaacc ggacgcagaa tgtctttgta tcggccggtc
      541 acctggtaat cgaggcccgg caggaatcct ttggcggcaa taactatacc tcgggccggc
      601 tgacgacgca gaacaagcag gccgttacct atgggcggat cgatatccgg gccaaactac
      661 cggtggcgaa ggggatgtgg ccggcattgt ggatgttggg ttccaacata acgacggtgc
      721 cctggcccgg ctgcggggag acggatatca tggaactggt gggcagttcg cccaaccagg
      781 tgacgggatc gttacactgg caacaggggg gtggcagcga gggtacgtac aacaacaatt
      841 atgatcttgc gtccgggcag gatttctcgc agcaattcca tgtttttagc ctgatctggc
      901 agaacaacag tgtgcagttc ctggtggatg accatgttta tgtgagtgga ggaatcgcca
      961 atgtcaccag tggcacctat ccttttaaca gccctttttt ctttatattc aatgtggcgg
     1021 tgggtggcga ctggcccggg gcgcccgatg ggacgacgag ctttccgcag cggatgttcg
     1081 tggactatgt acgggtattc cagcaatagg gcataaatgc gtttatatga aaaccagcaa
     1141 gacggtatgg tttgggatac ttatcctgtc ccttggcggg ttgaccgcct gcagcaagaa
     1201 aagcagtaac ccggcccctc cggtaacgcc gcctgcggat accacgaaga cgcctgtgaa
     1261 ttcccaggtg gcgttttggt tgacgaaccc tgacaagacc gcgctgttcc agcaacagaa
     1321 tatcgcgttg aactttggca gcgggcttgc cgtggagccg gtgatcacgg tggactcgac
     1381 ccaggcttat cagaccatcg atggttttgg gtatgcactg acgggtggaa gtgcgcagct
     1441 tatgtatagt ttgccggcgg cgcagcgggt ggccctggaa caggagttgt ttggaacgga
     1501 cagtacgcat atcggggtaa gctatctgcg ggtaagcatc ggggcttcgg atatgagcgc
     1561 ccgggtcttt tcctatgatg acaatgccag tcctgcacag cccgatacga gccttgccag
     1621 tttcgatctt tcggatgaca agatctatct ggttccgttg ttgaaggaga tactgaccat
     1681 caatcctacc atcaagatac tcggttgtcc ctggagtgcg ccggtctgga tgaaggacaa
     1741 taacaacagc aaggccgggt cgctgctatc gcagtattat ggggtgtatg cgaattattt
     1801 tgtgaagtat gttcttgcga tgaaggccaa cggtatcgcg atcgatgccg tgacgcctca
     1861 gaatgagccg ttgaatgcga acaacaatcc cagcatggta atgcaggata ccgccgaaga
     1921 gcggtttgtc cgggattggc tggggccggc gttcaaggtg gcgggcatcg ggacgaagat
     1981 cattgtatgg gatcataact gtgatgtgcc ttcttatccg ctgacgatcc tggcggatgg
     2041 ggctgcggcg caatatgtgg atggcagcgc ttttcacctg tatgcagggg atatttcggc
     2101 gctttccacc gttcataatg cctatccgaa caagaacgtt tattttacgg aacaatatgt
     2161 cgggggtccg gggaattttg cgtccgacct ggattgggcc atcaagaatc ttatcatcgg
     2221 ggctccgcgt aactggagcc ggaatgtgct cgagtggaac ctggccgccg atgccagcta
     2281 tgggccgcat accagtggag gatgcggggt ctgccagggg gcgttcacca tcgacggaac
     2341 gacggttggc cggaatactt cctattatat cattgcgcat gcgtccaagt ttgtgcggcc
     2401 gggttcggtg cggatcagtt cgaatcttac cggtaacctg caaaatgtgg cgtttctgac
     2461 accggggggg aagaaggtgc tgatcgtgtt gaatgataat gccgctgcgc agacgtttac
     2521 ggtgcaattc aacgggaaga atttttcgac gacattgaat gggaatgcgg tgggtacgta
     2581 tgtatggtag gagtgggggg atggagggga agaggatttg tgtacctttg cgcccatgac
     2641 gaaagtatat ctgaagaaaa agatcagccg gcgggtggag aacgggcatc cgtggatatt
     2701 tggaaatgag gtgggtacgg tggaggggga cagtgccggc ggcagtattg tggatgtttt
     2761 tacgcatgac aagaaattcc tgggcagggg gtatatcaac ctgcaatccc agatatcggt
     2821 gcggcttctg acgcgggaca aggccgagac catcgatgac gctttttttt attcccggtt
     2881 aagcgaggcc tgggactatc ggcggcggtt ggggtacacg gagaactgcc ggcttgtgtt
     2941 tggagaggcg gattacctgc ccgcgctgat catcgataaa ttcaacgact attttgtttt
     3001 acagaccctt gcgttgggga tggatcgctg gaagccggct atcgtcgatg cgttgcagcg
     3061 gatctttcag ccgaaaggga tttacgagcg gaatgatgtg ccggttcggg aactggaggg
     3121 gctgccccag cagaagggat ttttgtctgc gccgttcgac acgcagatcg tcatccggga
     3181 aaatgggttg aagctgcatg tcgatatcga gaatgggcag aagacggggt atttcctgga
     3241 ccagcatgac aaccgaaggg ctatccagca tatcgtgaag ggcgcggatg tgcttggggc
     3301 tttttgctat acgggcagtt ttgagacgca tgccggcttt tatggtgcga aatcggtttt
     3361 ggggctggat atttcggaga atgcggtagt gcaggcccgg cgcaatgcgg agctgaacgg
     3421 gcttggcgag gtgtgccgtt atgaggctgt aaatgcgttc gacgccctga aacagtggag
     3481 caaggatggg cgtcagtatg atgtcgtcat gctcgacccg cctgcattca cgaagagccg
     3541 ggagacgatc cagaaggcca tcaccggata taaggagatc aacctgcggg ggatgaagct
     3601 ggtcaggaag ggtgggttcc tggtaacgtc ttcctgtacg aatctggtcc aaccggaatt
     3661 attcctggaa accatccagc ttgccgcaaa ggatgcccgg cgcaggttgc gtcaggtgac
     3721 gtttcaggcg caggccagcg accaccccat catcgggggt tgggagaaca ccaattacct
     3781 gaaattcctt atcgtgcagg tgctctaggt ctttatcttc ctgttatggt gtaatagaat
     3841 ttcgtgcaat accgcttcag caggaaggcg atcgcgaagg cgaggatgat gaccacgacg
     3901 acggagccgg ccaggaacag gaagaggaag gcatgatagt gcaggtatct ttccgcgcca
     3961 agcttcaaca gcccccgttg cacgaagcta aacaggaaca tattaatggc gaacaggaag
     4021 aaggaatagg gcttgtattt attaaggtat ttgaatacaa ggctattctg gttactatcc
     4081 aggatgttaa agacggagag caggaagaag aatacgacac acagctttat ttcgaggacg
     4141 gggaacaggt tgtccttcca gttggaagtg gacagggcga cccaggtgag cacggggatc
     4201 aggataaggg ttgcgagttt gctgaccggt atcctgggca ggaatttctc gttgtagccg
     4261 aagtagcagc ccatgaggta ataggggagg aaccgggatt gaaaatagag gtctgccagg
     4321 ttaaaagagt agcaggcgag gaccaggaga aagaatgcga agaagacata cttgttcttt
     4381 agcaggtagt aaatgatgaa attgaagggg agtatcagta ccaggttttg gaggtaccag
     4441 aaattgacca ggggtggatt gatgatgttc aggagtacgt ttttgaaggt caggctttct
     4501 tcgggagcgt accagaaggt ggaatggaag aggcgggtgt acacatcttt taccacgatg
     4561 tagacgacga agaaggacag tgtccagaac agatagggga cgacgaggct atggaagcgg
     4621 gtctttagct ttttcaggta atggaagtct tcctgtgtcc ggtagaagaa gaggtagccg
     4681 gagagaacgg ccaggcaggg tgaggtcaga tcgaaccaaa ggtcgaagat cgtgtggtcg
     4741 tagggtgcgt tggcgaggta aagattgatg ttccagtgat tgatcagaac aagatgtatg
     4801 acgagaaaac gaatctcatc aatgtgctgc aaattgcgtt tcatgtacga tggcttaggg
     4861 gtatagaata aaac
//
LOCUS       DATGFG010000961         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_3285, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000961 DATGFG010000000
VERSION     DATGFG010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3285"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VKQ52_18120"
     CDS             complement(<1..312)
                     /locus_tag="VKQ52_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89181.1"
                     /translation="MTKPSFRLAIVSTHPIQYNAPWFRELASKIELMVFYTWSQSKEG
                     AKYDPGFGRVITWDIPLLEGYPYTFVENISPEPGTHHFRGIVNPSLNETIGEWQPDAI
                     LI"
     gene            complement(309..1859)
                     /locus_tag="VKQ52_18125"
     CDS             complement(309..1859)
                     /locus_tag="VKQ52_18125"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLZ89182.1"
                     /translation="MPFIDYNTTPFGFDPAAYDVIIVGAGAAGILLAVELTRKHTRVL
                     LVEAGHFTEDQDRQVLNEVEQTGKTVTTAVWGRKRAIGGTTIAWGGQSLPFGPIDFEK
                     RSWVANSGWPLAYEELEPYYVAANRFMNIDELDYEDDILRLLKMKRPGFDEKELHHHF
                     SKWAPEPDFRKMYEGYLLANVTVLYNAVLTRLHDDGQSMITGLTITNFRQEQMRIHAP
                     TVILATGGIEANRTLLNQRQLAGDGPARFSEWLGKCFMEHPCIEVGDVLTDHPWRLQK
                     NFNTHIRNHRKYSIRFSLAAEAQRQRRLLNGSASILFQYDNEKHDPYLEVKQFLRQGR
                     KISLKRLPVHHLKALGLGAASLALHHFIYKHKSHPKLIMMMEQEPIEDSYIGLSAEKD
                     RFGLPRAAMHWKITPNTWRTVVHLSRMAKTEIEKGGFGKVALYDHIDEQEPAWANYLS
                     DVNHHMGGTRMSTTAAEGIVDTDLRVWGFSNLFLCSTSVFPTGSHSNPTLTLLALAQR
                     LANKLSTT"
     gene            complement(1861..2796)
                     /locus_tag="VKQ52_18130"
     CDS             complement(1861..2796)
                     /locus_tag="VKQ52_18130"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLZ89183.1"
                     /translation="MPESSINYLGFGTAGITSLPSRRAAIRLLRTAFDAGIRHFDTAP
                     LYGKGYAEQLLGDFAVGRRPAITITTKFGLGESGALPLPAFLALPLNYYRKKWKGAAA
                     PGTGEPAGSGQSVQGAPGQGSHAGPGQGAGPEAHPLPYRKIDRASIEKSLAASLQRLR
                     TDYIDYYFLHEGLPGFLDPDALEFLLDRKQKGVIRRLGVATDGFNLGSLGPRDLDGWD
                     VLQYEAGEWQAPLRKRYPDKLHFVHSVLKHFDRQAAEPGVAGGTGGAGVAAEASAGRM
                     LAAQKKTGLADKLLFSTRQRPRLQQNIAGFIIGSC"
BASE COUNT          574 a          920 c          792 g          595 t
ORIGIN      
        1 gatcaggatc gcatccggct gccattcccc gatggtctcg ttcagcgagg ggttgacgat
       61 cccgcggaaa tgatgtgtgc ccggctccgg ggaaatgttc tccacaaagg tatagggata
      121 cccttccagc aacgggatat cccaggtgat gaccctgccg aaacccgggt cgtatttggc
      181 gccttccttc gattgcgacc aggtatagaa taccatcagc tctatcttcg aagcaagctc
      241 cctgaaccag ggcgcgttgt actggatagg gtgagtggac acgatggcca gtcgaaagga
      301 cggttttgtc atgtagtact gagcttattt gccagccgct gggcaagcgc cagcagcgtc
      361 aatgtcggat tggaatggga accggtggga aagacggagg tactgcaaag aaacaggttg
      421 ctgaatcccc agacccgcaa atcggtatcg acgatcccct ccgccgccgt cgtactcatc
      481 cgggttcccc ccatatgatg attcacatcg gaaagataat tggcccaggc cggctcctgt
      541 tcatcgatat gatcgtagag cgccaccttc ccaaatcctc ccttctcgat ctccgtcttc
      601 gccatgcggg acaggtggac cacggtgcgc caggtattgg gcgtgatctt ccagtgcatg
      661 gccgctctcg ggaggccaaa gcggtccttc tcagccgaca atccgatata gctgtcctcg
      721 atgggctcct gctccatcat catgatcagt ttcggatggc tcttgtgttt gtagataaaa
      781 tgatgcaggg ccagcgacgc cgcgccaagc cccagggcct tcagatgatg caccggcaat
      841 cgcttcaggc tgatcttcct cccctgtctc aaaaattgtt tcacctccag atacgggtca
      901 tgcttctcat tatcatactg gaaaaggatg ctggccgatc cgttgagcag ccggcgctgc
      961 cgctgggcct ccgcggccag gctgaaccgg atggaatatt tccggtggtt ccggatatgc
     1021 gtgttgaaat tcttctgcaa tcgccacggg tgatcggtca gcacatcccc gacttcgata
     1081 cagggatgct ccataaaaca tttccccagc cattccgaaa agcgggccgg accgtcgccg
     1141 gccagctgcc gctggttgag cagcgtacgg ttcgcttcga taccgcccgt cgccaggatc
     1201 accgtcggtg catgtatccg catctgttcc tgccggaaat tcgtgatggt caacccggtg
     1261 atcatcgact ggccgtcgtc atgcagccgc gtcagcaccg cgttgtagag cacggttacg
     1321 ttcgcaagga gatacccctc gtacatcttc ctgaaatccg gctcgggcgc ccacttggag
     1381 aaatgatgat ggagctcctt ttcatcaaac cccggccgct tcatcttcag cagccggagg
     1441 atatcatcct cgtaatccag ctcgtcgata ttcatgaacc ggttcgcggc gacataatag
     1501 ggctcgagct cctcataggc gagcggccag ccgctgttgg cgacccagga gcgtttctcg
     1561 aagtcgatcg gcccgaaggg aagggattga ccaccccagg cgatcgtcgt gccgccgatg
     1621 gcccgctttc ttccccagac cgccgtggtc accgtcttgc ccgtctgctc cacctcgttc
     1681 agcacctgcc ggtcctggtc ctccgtgaaa tgcccggctt ccaccagcaa cacgcgcgta
     1741 tgcttccgcg ttagctccac ggccagcagg atgcccgcgg cgcccgcgcc gacgatgatc
     1801 acgtcgtagg ccgccgggtc gaagccgaaa ggggtggtat tataatcgat gaatggcatg
     1861 ttaacaggat ccgatgataa agcccgcgat attctgttgc agccggggcc gctgccttgt
     1921 gctgaagagc agcttatccg ccaggcccgt ctttttctgc gccgccagca tcctgcccgc
     1981 gctcgcctcc gcagccacac ccgccccacc cgtccccccg gcaacacccg gttccgcggc
     2041 ctgccggtcg aaatgcttca gcacactatg gacaaaatgc agcttgtccg ggtatctctt
     2101 tcttagcggc gcctgccatt cccccgcctc gtattgcaac acatcccagc catcgagatc
     2161 ccggggacca agactgccga gattgaagcc gtccgtggcc acccccagcc gccggatgac
     2221 gcccttttgc ttccggtcca aaaggaattc cagcgcatcg ggatcgagaa aaccgggcag
     2281 gccctcatgc aggaaataat agtcgatata gtccgtcctc agccgctgca gactcgccgc
     2341 caggcttttt tcaatactgg cccgatcgat cttccggtaa ggcaacgggt gagcctccgg
     2401 ccctgcaccc tgcccgggac ctgcgtgcga accctgcccg ggagctccct gcacactctg
     2461 ccctgaacct gccggctcac ccgtcccggg agcagccgct cccttccatt tcttccggta
     2521 atagttcaac ggcagcgcca ggaaggccgg caacggaagc gcgccgcttt ccccaagccc
     2581 gaacttggtg gtaatagtga tggccggccg gcgacccacg gcaaaatcgc ccagcaactg
     2641 ctcggcatac cccttcccat aaagcggggc cgtatcaaaa tgccggatac cggcatcgaa
     2701 ggcggtgcgc agcaaacgga tagcggcccg gcgggaaggc aggctggtga tacccgcggt
     2761 cccaaagccc agatagttga tggaggattc cggcatagtt taacgttgca gggtttgatc
     2821 gagcaggccg gccagtttct ccgtcacctt gtaagcacta tattccgaga aaagttccct
     2881 g
//
LOCUS       DATGFG010000962         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_282590, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000962 DATGFG010000000
VERSION     DATGFG010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282590"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VKQ52_18135"
     CDS             <1..839
                     /locus_tag="VKQ52_18135"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89184.1"
                     /translation="LLFAFAAAGASGVAIRVRDLKSKPYIDLTLQVMKHFGWEIENRE
                     YAEFRFPVERGASGATGDQTIYTVEGDWSGGAFLLVAGAIAGPVHVRGLDEWSTQADK
                     AILQALCDCGASLEMREEEMVIGPAGGSDGDGAAGAGVQKAAGAGLTAFQFDATDCPD
                     LFPPLVALAAYCSGVTVIRGVSRLAHKESNRGLTLQEEFGKMGVRVDLDGDVMRVHGG
                     GAVSGATVHSRHDHRIAMSCAVAALKAAGPVRIEDAEAIDKSYPDFYRDIAALGGKVS
                     GI"
     gene            870..1865
                     /locus_tag="VKQ52_18140"
     CDS             870..1865
                     /locus_tag="VKQ52_18140"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HLZ89185.1"
                     /translation="MNTFGRFFRISIFGESHGESVGVTIDGCPAGLPLPLEDFLPDLE
                     RRKGGMQKGTTPRKEDDLPIFKSGIFNQVSTGSPITILFENNNTRSGDYEKQRAIPRP
                     GHADFVAHTKFGGFEDYRGGGPFSARLTVGLVAAGVIAKKILGGVTVTSTILEIGGEA
                     DLDKGLQKAIDAKDSIGGIVECRVSGLPIGLGEPFWDSVESLLAHAAFAIPAVRGVEF
                     GTGFAAARMFGSQHNDAIEDMQGHTRTNHAGGVVGGITNGNELVFRIAIKPTSSTPKE
                     QQTLNWETGQQEAFSVKGRHDLCVALRAPVILEAVTAIVLADLWLLEQRAPRVVK"
     gene            1925..>2966
                     /locus_tag="VKQ52_18145"
     CDS             1925..>2966
                     /locus_tag="VKQ52_18145"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HLZ89186.1"
                     /translation="MNYRKRSVLFLFPMISMMAITACHEQSKHPGVDKDEVKTPAQVD
                     EHAGTDLKSMLQYAADNGDKLNDSTVLVYRKLADSVYGSNGYTPIWSDKQRWLPAGDS
                     LLSLIEGCKVYGLFPSDYHYASLSFFRRVIGEDTLARKNAATWNRADLLLTDAFFMLV
                     KHLRQGRLDYDSVTLRRDSVLPDSFFTRTLATARQFGVRGALDSLEPRHPGYDSLKAY
                     LGAFLAGAHFRKLTWLDYPYKDSAVFYTALTRRLQEVGYIDSSVTLADTAMLASALRK
                     YQETNKLKVTGKASQETVTMMDNSDWEKFKRIAINLDRYKLLPDTLPRTYVWVDLPAF
                     SLQVRDADTVALE"
BASE COUNT          599 a          681 c         1039 g          647 t
ORIGIN      
        1 ggctgctgtt tgcgtttgcg gcggcgggtg cgtcgggtgt tgcgatccgg gtccgtgacc
       61 tgaagagcaa accatatatc gatcttacgc tgcaggtgat gaagcatttt ggctgggaga
      121 tcgagaaccg ggagtatgcc gagttccggt ttcctgtgga gaggggcgcc agcggagcga
      181 caggggatca aactatttat accgtggaag gagactggag tggaggggct tttctgctgg
      241 tggcgggagc cattgccgga ccggtgcatg ttcgtggtct ggacgagtgg tcgacccagg
      301 cggataaggc catattgcag gcgctttgcg attgtggggc cagcctggaa atgcgggaag
      361 aggaaatggt tattgggccc gcgggtggaa gtgacggcga cggtgcggcc ggtgcaggag
      421 tgcagaaggc tgcgggtgcg ggcttgactg cttttcaatt cgatgcgacc gactgtcccg
      481 acctgttccc gccgctggtg gcgctggctg cgtattgttc cggggtgacg gtgatcaggg
      541 gggtgagccg gctggcgcat aaggaaagca atcgtgggct gaccctgcag gaggagtttg
      601 ggaagatggg ggtgcgcgtc gacctggacg gagatgtgat gcgggtgcat ggaggcggcg
      661 ccgttagcgg ggcgactgtc cattcccgtc atgatcaccg gatcgccatg tcctgtgcgg
      721 tcgcggcgtt gaaggccgcg gggccggtga ggatcgaaga tgcggaggct atcgataagt
      781 cctatcccga tttttaccgc gatattgccg cactgggcgg aaaagtgagc ggcatataga
      841 cgattgggtg ggaaagtaaa aatacagtta tgaatacctt tggccggttt ttccggatca
      901 gcatttttgg cgaatcccat ggggagagtg taggggttac tatcgacggt tgtccggcgg
      961 gattgccgct gccgttggag gattttttgc ccgacctgga gcgccggaag ggcgggatgc
     1021 agaaggggac gaccccccgg aaggaggatg acctgccgat cttcaagagc gggatcttca
     1081 accaggtgag tacggggtcg ccgatcacga ttctttttga gaacaacaat acccggagcg
     1141 gggattacga aaagcagcgg gcgataccgc ggccggggca tgccgatttt gtcgcgcata
     1201 ccaagtttgg cgggtttgag gattatcgtg gcggcggtcc gttctccgca aggctgacag
     1261 tagggctggt ggcggcgggg gtgatcgcca agaaaatact ggggggagtg accgtgacct
     1321 cgacgatcct ggagatcggg ggggaggccg acctcgacaa ggggttgcaa aaggccatcg
     1381 atgcgaagga ttccatcggc gggatcgttg aatgcagggt cagtggattg cccatcgggc
     1441 tgggggaacc tttctgggat tcggtggagt cactgctggc gcatgcggct tttgccattc
     1501 cggctgtcag gggtgtggag tttggtaccg gttttgcggc cgcccggatg tttggcagcc
     1561 agcacaatga tgccatcgag gacatgcagg gccatacccg gacgaatcat gcgggcgggg
     1621 tagtgggggg gatcaccaac gggaatgagc tggttttccg gatcgcgata aagccgacct
     1681 cttcgacgcc caaggagcag cagacgctga actgggagac ggggcagcag gaggcgttct
     1741 cggtgaaagg gcgtcatgat ctttgtgtgg cgttgcgggc cccggtcatc ctggaggcgg
     1801 tgacggccat tgtgttagcg gatctctggt tgctggagca acgcgcgccg agagtggtga
     1861 aataaccaaa aaatagcggt ttacactcag aaatattggc gcgttgtatt atattgggtc
     1921 aaatatgaac tatcgaaagc gaagcgtcct tttccttttt ccgatgattt cgatgatggc
     1981 gataacggcc tgccatgaac agagtaaaca tccgggagtc gataaggacg aggtgaaaac
     2041 cccggctcag gtggatgaac atgcaggaac cgatctgaag tcaatgctcc aatatgcggc
     2101 ggacaacggg gacaagttaa acgattctac tgttttggtt taccgcaagt tagcagacag
     2161 tgtttatgga agcaacgggt atacccccat ctggagcgat aagcaacgat ggttgccggc
     2221 gggggatagt ctcttgtcgt tgatagaagg ctgtaaggtt tacggtctgt ttccctcgga
     2281 ttatcattat gcctctttgt cttttttccg gcgggtcatc ggggaggata cgctggcgcg
     2341 gaagaatgcc gccacctgga acagggccga tctgctgctg accgatgcct tttttatgct
     2401 ggtgaagcat ctcaggcagg gccggctgga ttatgacagc gtcacgctgc ggcgggattc
     2461 ggtgctgccg gatagttttt ttacgcggac gctagcgacg gcacggcaat ttggggtcag
     2521 gggcgctctt gacagcctcg agccccggca tcccggctat gactcgttaa aggcttacct
     2581 gggggctttc ctggcgggtg cgcatttccg gaagctgacc tggctggact atccgtataa
     2641 ggattcggcg gtgttctaca ccgccctgac gaggcgtttg caggaggtgg ggtatatcga
     2701 cagttcggtg accctggccg atacggccat gcttgcaagc gcgttgcgca aataccagga
     2761 gaccaataaa ttaaaagtga cgggcaaggc gtcacaggag acggtgacga tgatggataa
     2821 ctccgactgg gagaaattca agcggattgc gatcaacctc gaccggtata agctgctgcc
     2881 ggatacgctg cccaggacct atgtctgggt ggatctgccg gccttttcct tacaggtgcg
     2941 ggatgcggac acggtggccc tcgagt
//
LOCUS       DATGFG010000963         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_551556, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000963 DATGFG010000000
VERSION     DATGFG010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551556"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VKQ52_18150"
     CDS             <1..854
                     /locus_tag="VKQ52_18150"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLZ89187.1"
                     /translation="ARRQHQEANLDEYTVSSRQPIASCSKWLSAALVMTFVDEGRLSL
                     DDTVGKYLPVLAAHGKGGITVRQCLSHLTGIKEPPMRESLSEMRKITSMDQAMEMIAE
                     MPMEGEPGKVFHYSNAGLQIAGAVLEKISGQSFETLFAGRIARPLGMANTDFGKGPVA
                     MPAGGAFSTPEDYIHFLEMILHRGMYHGTRILTERSVQEMQVNRLGPDVRIAYTPAEN
                     GGAGVGYGFGEWLMGEAVSSPGLFGSFPWVVNGRGYCAFLLTVNLNNKGRQDREMELV
                     RLVDESL"
     gene            880..2211
                     /locus_tag="VKQ52_18155"
     CDS             880..2211
                     /locus_tag="VKQ52_18155"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ89188.1"
                     /translation="MKILLLVLLWVSGLVMVRAQMPAMAQDKPASYLIKAGKLFDSET
                     GEFKTGMVILVKDKKIEAVRSEKEVSEAEKEGRQVVDLSAYAVLPGLIDCHTHLLYRE
                     VLHPGNTVESMDLVKELTTEGDAYRAIYGTARAKAYLEAGITSVQDLGNSGQYADMAL
                     RRAINEGLVPGPRMRCAGRGLSTEGGQLPGVIYKHQNLVADEYRVVKGPDDAVQAVRE
                     NITQGADVIKIYSNNTPNNTMLSVEEIRAIVGEAHRYGLRVTAHATDNRAVYNAVIGG
                     VDGIEHGYSVDDTTFQLMARKGVIFVPTDGDSLIFTRYAQLANPDDKSGGARVMGFRR
                     YLGLRLRAALRAGVTVAAGSDDYVDVKLPFADLSKHTIIGYVESGVPVVRALQFATIN
                     ASKQLNWVGEVGVLKRGAFADIIAVDGDLDMDIHALMKVHFVMKGGAVVVQ"
     gene            complement(2205..>3582)
                     /locus_tag="VKQ52_18160"
     CDS             complement(2205..>3582)
                     /locus_tag="VKQ52_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89189.1"
                     /translation="VYGLANEALGHYTDAKDGFDIATLDPATRSAAYTCLARLSLKQD
                     DPARAKGYAEKAMVNNALNMEALQLQAVCARLSDRNAPPPVNSNRNVLPPVNPNRNAL
                     PPVNPNRPGNKDILALIDSLDPLNHFSRFERYLADPGPSNKQAFLSLIRNELPHETFL
                     ELGIWYYNCGLTNEALQVFRLSPPNAEVSLWIARLTGTPVDWSAIDPVRAFPFRSETG
                     ALLEERLKTDNHWFLKYQLAYIYRDRNRLDDCKRLLLSCADTPGYAPFYVFRAQTMPE
                     DSQCLHDLLHARSMDPGWRYQKLLTEYYIRNKDYPRALAVIEAYCRSHPDHNTMGLLY
                     ARTLVLNHQWQEADRLLAQLQVLPAEGSTSAHDIYREVKLMEAVGKIKRKEYKKARAL
                     IADARQWPENLGSGAPYPADQDTRLEDYLGSLCREIAASPASAPAPPEPATPIEHRVL
                     QALTGAGY"
BASE COUNT          670 a          728 c         1392 g          792 t
ORIGIN      
        1 ttgcgcggcg gcagcaccag gaagcgaatc ttgatgagta tacggtgtcg agccggcagc
       61 cgatcgcgag ttgcagcaag tggcttagcg cggcgctggt gatgaccttt gtggacgagg
      121 ggcgattgag cctcgatgat acggtgggaa agtatttgcc ggtgcttgcg gcgcatggaa
      181 aaggtgggat cacggtgcgg cagtgtctgt cgcacctcac gggcatcaag gaaccgccta
      241 tgcgggaaag tttgagtgag atgcggaaga tcacgagcat ggaccaggcg atggagatga
      301 tcgcggagat gccgatggag ggggagccgg gaaaagtatt ccattacagt aacgccggtt
      361 tacagattgc gggggcggtg ctggagaaga tcagcggcca atctttcgag actttgtttg
      421 ccgggcggat agcccgaccg ctgggaatgg ccaatacgga ttttggcaag gggccggtgg
      481 cgatgcccgc ggggggcgct ttcagtacgc cggaggatta tatccatttt ctcgagatga
      541 tcctccaccg gggtatgtat catggaacgc ggatccttac cgagcggagt gtgcaggaga
      601 tgcaggtgaa ccggcttggg ccggatgtgc ggatcgcgta tacgccggcg gagaacggcg
      661 gtgcgggtgt cggctatggc tttggggagt ggctgatggg tgaggcggtg agcagtccgg
      721 ggctttttgg gagtttcccg tgggtggtga atgggcgggg ctattgtgcc tttctcttaa
      781 cggtgaattt gaataataag gggcgtcagg accgggagat ggaattggtg cgattggtgg
      841 acgaatcgtt gtaaattggg gggcaaaaac ctcctgttta tgaaaatatt gcttcttgta
      901 ttgctttggg tgtccgggct ggtgatggtg cgggcgcaga tgccggcgat ggcgcaggat
      961 aagcctgctt cctacctgat aaaggccggt aaacttttcg acagtgagac gggggagttc
     1021 aagaccggga tggtcattct tgtaaaggac aagaagattg aggccgtcag gtccgagaag
     1081 gaagtgagcg aggcggagaa ggaggggcgg caggtggtgg atctttccgc ttatgccgtg
     1141 ttgccggggc ttatcgattg tcatacgcat ttgctgtacc gggaggtgct tcatccgggg
     1201 aataccgtag agtcgatgga tttggtgaag gaattgacga cggaggggga tgcctatcgg
     1261 gcgatctatg ggacggcgcg ggcaaaggcc tacctggaag cggggatcac atcagtgcag
     1321 gatcttggga attcgggtca gtatgcagat atggcgttgc gccgggcgat caatgagggg
     1381 cttgtaccgg ggccgcggat gcggtgtgcc ggtcgcgggc tttctacgga aggcggccag
     1441 ctgccgggag tgatctataa gcaccagaac ctggtggcgg atgagtaccg ggtggtgaag
     1501 gggccggatg atgccgttca ggcggtgagg gagaatatca cgcagggggc ggatgtgata
     1561 aagatctatt cgaacaatac gccgaacaac acgatgctga gtgtggagga gataagggcg
     1621 atcgtcgggg aagcgcaccg gtatgggctt cgggtgacgg cgcatgccac ggataaccgt
     1681 gcggtgtaca atgccgtgat cgggggtgtg gatgggatcg agcatgggta tagtgtcgac
     1741 gacacgactt ttcagctgat ggccaggaag ggagtgatct ttgtaccgac ggatggagac
     1801 agcctgatct ttacgaggta tgcacagctg gcgaatccgg atgacaagag tgggggcgcc
     1861 agggtcatgg gtttccggcg atatcttggg ctgcggttaa gggctgcgct gcgggcgggg
     1921 gtgacggtgg cggcaggttc ggatgactat gtggatgtca agctcccatt tgcggacctc
     1981 tcgaagcata cgatcatcgg gtatgtcgaa tcgggggtgc ctgtggtgag agcattgcag
     2041 tttgcgacca tcaatgcgag caagcaattg aattgggtcg gggaggttgg tgttttgaag
     2101 cggggggctt ttgcggatat tattgccgtg gatggggatc tggatatgga tatccatgcc
     2161 cttatgaagg tgcattttgt gatgaagggg ggtgcggtgg tggttcagta gccggcgccg
     2221 gtgagggcct ggagcacgcg gtgttcgatg ggagtggcgg gttccggtgg cgccggcgcg
     2281 gaggcaggag aagcggcgat ctcccggcag agtgagccaa ggtagtcttc gaggcgggtg
     2341 tcctgatcgg cggggtaggg ggcgccggag ccgaggtttt cgggccattg tcttgcatcg
     2401 gcgatgaggg cccgggcttt tttatattct tttcttttta ttttccctac agcttccatg
     2461 agttttactt cacgatagat gtcgtgggcc gaggtagaac cttcggcggg gaggacctgc
     2521 agttgggcga gcaggcggtc tgcttcctgc cactgatggt tcaggaccag ggttcgggca
     2581 tagagcagcc ccatggtgtt gtggtcggga tgggaccggc aataggcttc gatgacggcg
     2641 agtgcccggg gatagtcctt attccgaata tagtattccg ttagtaattt ctggtagcgc
     2701 cagcccgggt ccatcgagcg ggcgtggagg aggtcgtgga ggcattggct gtcttcgggc
     2761 atcgtctgtg cgcggaagac gtagaagggg gcgtagccgg gggtatcggc gcaggatagc
     2821 aggaggcgtt tgcaatcgtc cagccggttg cgatcccggt agatataggc caattggtat
     2881 ttaaggaacc agtggttgtc ggtctttagc cgttcttcga gcagggcgcc ggtttcggaa
     2941 cgaaagggga aggcgcgaac ggggtcgatg gcggaccagt cgacgggtgt gccggtgagc
     3001 ctggcgatcc agaggctgac ctctgcgttt ggaggggaga ggcggaagac ctgcagggct
     3061 tcgtttgtaa ggccgcagtt gtagtaccag ataccgagtt caaggaaggt ttcgtggggg
     3121 agttcattac ggatcaggga gaggaaggct tgtttgttcg agggacccgg gtcggcgaga
     3181 tagcgttcga agcgggagaa gtggtttagc gggtcgaggc tgtcgatcag ggccaggatg
     3241 tctttgttac cggggcggtt gggattgacg gggggcaggg cgttccggtt gggattgacg
     3301 gggggtagga cgttccggtt ggaattgacg gggggcgggg cgttgcggtc ggagaggcgg
     3361 gcgcagacgg cctggagttg gagggcttcc atgttgaggg cgttgttgac catcgctttt
     3421 tcggcatagc cttttgcgcg ggcggggtca tcctgcttca ggctgaggcg tgccaggcag
     3481 gtgtaggcgg cgctgcgggt tgcggggtcg agggtggcga tgtcgaatcc gtctttggcg
     3541 tcggtgtagt ggccaagggc ttcgttggcg aggccgtaga cg
//
LOCUS       DATGFG010000964         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1537977, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000964 DATGFG010000000
VERSION     DATGFG010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537977"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VKQ52_18165"
     CDS             <1..326
                     /locus_tag="VKQ52_18165"
                     /inference="COORDINATES: protein motif:HMM:NF016139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HLZ89190.1"
                     /translation="RSPAEALVTNLHRITIGHFIRIQCFLVTLGIIECTIGILWLVPK
                     FTKWVIVVFFSQMFTTFLPLFIMRDQTWSDFAVLSLSGQYILKNIVLVACALTIYKDY
                     RLRSS"
     gene            complement(441..2906)
                     /locus_tag="VKQ52_18170"
     CDS             complement(441..2906)
                     /locus_tag="VKQ52_18170"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLZ89191.1"
                     /translation="MAPDLKKVLLILCFTVGVVVGYTQDKGFVEGRVRAGPGQPIPAA
                     TVTLVNVDDPTEKKTEQTDTAGRFLFEGIPYGQFTCRITHAGYLPVTRDSIVITPISG
                     TINLGDLTLIPSSELLTEAIVSAKNPTLRAGQEKKVFSVNQSLVSTGGSAIDLLQNVP
                     TLQVDASGNVSLRGATNLKVLVDGKRSLIGDGSIPQVLQSLPASAIDRVEIITNPSAK
                     YDAEGQALINIVLKKNSIAGSSGSVAVTEGTRDNYNAAVTMGYQNSRINAYGNYSYQR
                     RNTYSNGIQNMTFLSSPGDAYYSDETFPSTTIVLLHSAKAGFEYSATARDVVTVSGSY
                     NASSRNRSERLTVDNLTADRAPVVLSTRQNGTTGHADSYEAALDLTHKFRQPQQELTI
                     DLDYSRGITHYRQLYATDVYDLYGQPADSTASLQDSKESHTRNYNIQLDFTSPAGKSG
                     KWEGGYRSQISIEDNSQWDANFNATSGDYAPDYTLINRFTSNSQVHAAYLTYRRQIHS
                     FSFQVGLRGEIGRFHANLSDFDSTGKPAVTPIAVNTQGLYPSLLLKEQIGEGRQLQVS
                     YSRRVNRPTPQELNPFSDVSDPVNYDEGNPRLLPEDLHSIELVFSRTWPKASLTSGLY
                     YNLANHVIKHIQTGPVNDVVITIAENLRRAVNTGIELIGTVRPVKGWDFTTNVNLFDR
                     INDGDSAFGIRATQGFSWNINLTNNFSLTRDLAIQLRADYKAPELIIQDRYRAAWGID
                     GGARYELWHHKGSLAFNARDIFNTRRPAFLRVSDDLLLDWHRITYSARASLTFTWRFG
                     HNDAGGQNRKGTEDQPVRRIENR"
     gene            complement(2912..3427)
                     /locus_tag="VKQ52_18175"
     CDS             complement(2912..3427)
                     /locus_tag="VKQ52_18175"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HLZ89192.1"
                     /translation="MKHIQQLLFTGLTLLCTLTGSAQTAKPRVSPAQSVSGTVNGATI
                     TIHYSSPSVKGRTIWGTLVPYDSVWRAGANEATTFETDKDIKVAGRPLPAGKYGFFLL
                     PRADGKWTAIFNTVPNQWGAFKYDQSKDQLRVEVSAKTINPAIEALNYQITQKGFALN
                     WEKISVPVSIK"
     gene            3595..>4032
                     /locus_tag="VKQ52_18180"
     CDS             3595..>4032
                     /locus_tag="VKQ52_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89193.1"
                     /translation="MRKRMSFTGKSTLTGLQYLVWAAILLIGFFAMVSSDGVKRSAAF
                     AVNNTCFYALIVYGNIRFLYPRLYQKKRYVPYVAGSVLLLMLAGAGRGYCSLIVRNEY
                     SLASSWWRDPATWLTFFLSGITVFILSFIFRLAIAYFLVKQASE"
BASE COUNT          786 a         1144 c         1108 g          994 t
ORIGIN      
        1 caagatctcc ggccgaggca ttggtaacca atcttcacag aattaccatc ggccacttta
       61 tcaggatcca atgtttcctg gttacactgg gcatcataga atgcaccatc ggaatattat
      121 ggttggtgcc taaattcaca aaatgggtaa ttgtagtctt cttctcccag atgttcacca
      181 ccttcctgcc tctgttcatc atgcgtgatc aaacctggag cgatttcgcc gtcctttcct
      241 tatccgggca gtatatcctg aagaatatcg ttctggtggc atgcgctttg accatttaca
      301 aagactatcg gctcaggagc agctgacagg actcactcca ccgccgtcac ctcacacatc
      361 cgtcccgcca ccgttacctc aatcgtctgc cccgccaccg tcacctcgca catctacccc
      421 cttctttgct tgaccacccc tcacctgttc tcgatccgcc gtaccggttg gtcttccgtt
      481 cctttcctgt tctgccctcc ggcatcgttg tgaccgaacc gccaggtaaa ggtcaggctt
      541 gccctggcgc tgtaggtgat acggtgccaa tccagcagga gatcgtcgct cacccgcaaa
      601 aaggccggcc ttcgggtatt gaagatatcc ctggcgttga acgccaggct tcccttgtga
      661 tgccacagct cgtaccgggc gcccccatcg atcccccagg cagcccgata cctgtcctgg
      721 atgatcagtt cgggcgcctt gtaatccgcc cgaagctgga tggccagatc cctggtgagg
      781 gagaagttat tggtgagatt gatattccag ctgaaccctt gcgtagcgcg gatgccgaac
      841 gccgaatcac cgtcattgat ccggtcgaac aggtttacat tggtcgtgaa gtcccagccc
      901 ttcacagggc gaacagtgcc gatcagctcg atgccggtat tgaccgcccg ccggaggttc
      961 tccgcaatgg tgatcaccac gtcattcacc ggaccggttt ggatatgctt gatgacatgg
     1021 ttcgccaggt tgtagtacaa tcccgatgtc agcgatgcct ttggccaggt ccggctaaag
     1081 acgagttcta tcgaatgaag atcctccggc agcagccggg gattgccttc atcgtagttc
     1141 acggggtcgg agacatcgga gaaaggattc agttcctgcg gtgtgggccg gttcactcgt
     1201 cgggagtagc tcacctgcaa ttgccggcct tcgccgatct gttctttcaa caacaggctg
     1261 gggtagaggc cctgggtatt taccgctatc ggcgtcactg ccggcttgcc ggttgaatca
     1321 aagtcgctga ggttggcatg aaaccgcccg atctctcccc gcagtcccac ctggaagcta
     1381 aagctatgga tctgccgccg gtaggtaaga taggccgcat gtacctggct gttgctggta
     1441 aagcggttga tcagggtata atcaggagca tagtcgccgc tggtggcgtt gaaattggca
     1501 tcccactggc tgttgtcctc aatgctgatc tggctgcgat agccgccctc ccatttcccg
     1561 ctcttgccgg ccggcgacgt aaagtcgagt tggatattat aattccgggt atggctttct
     1621 ttgctgtctt gcagactggc cgtcgagtcc gccggctggc catataagtc ataaacatcc
     1681 gtagcatata gctgacggta atgggtaatg ccccgggaat agtccaggtc gatggtcaac
     1741 tcctgctgcg gctgccggaa tttatgggta aggtccagcg ccgcctcgta gctgtcggcg
     1801 tgtcccgtag tgccgttctg ccgcgtgcta agtaccacgg gggccctgtc ggccgtaagg
     1861 ttatcgacgg tcaatcgctc gctgcggttc ctggaggacg cgttgtagct gccggaaaca
     1921 gtaacgacat ccctggccgt tgccgaatac tcgaaccccg ccttggcgga gtgcagcagc
     1981 acgatggtgg tggatgggaa ggtctcgtcg gaatagtagg cgtccccggg cgacgaaaga
     2041 aaggtcatgt tctggattcc attgctgtac gtgttccggc gttggtaact gtagttccca
     2101 tacgcgttga tccggctgtt ctggtagccc atggtcaccg ccgcgttata attgtcccgg
     2161 gtaccctcgg tcactgccac cgatccgctg cttccggcaa tgctgttctt cttcaacacg
     2221 atattgatga gtgcctgccc ctccgcatca tatttggctg aaggattggt gatgatctcg
     2281 acccggtcga tggcgctcgc cggcagcgat tgcaacacct gtggtatcga cccatcgccg
     2341 atgagcgatc gcttcccgtc gaccaacacc ttcaggttgg tcgccccccg caggctgaca
     2401 ttgccactgg catccacctg cagggtggga acattctgca ggagatcgat tgccgaaccg
     2461 ccggtgctca ccaggctctg gttgacggaa aagacctttt tctcctggcc ggcccgcagc
     2521 gtcggattct tcgccgaaac gatcgcctcg gtcagcagct cgctggaggg gataagcgta
     2581 agatcgccaa gattgatcgt cccgctgatc ggggttatga cgatgctgtc cctggtgacg
     2641 ggcagataac ccgcatgggt gatcctgcag gtgaattggc cgtagggaat gccctcgaac
     2701 aggaaacggc ccgcggtatc cgtttgttcc gtttttttct cggtcggatc atcgacgtta
     2761 accagcgtga cggtcgccgc gggaatgggc tggcccgggc cggctctgac ccttccctcg
     2821 acaaagcctt tatcctgggt atacccgacg accacgccga cagtgaagca taggatcaac
     2881 aggacttttt tcagatctgg ggccataggg tttattttat ggataccgga accgagatct
     2941 tttcccagtt gagagcaaat cccttttggg taatttgata attcagggcc tcgatagctg
     3001 ggttgatggt tttggcgctt acctccacac gcagctgatc ctttgattgg tcatatttga
     3061 aggcgcccca ctggttcggc acggtgttga agatcgccgt ccatttccca tcagcccggg
     3121 ggagaagaaa aaaaccgtat ttacccgccg gcaatggcct gcctgccacc ttgatatctt
     3181 tatcggtctc gaacgtagtc gcttcattgg ccccggcgcg ccagaccgag tcatagggta
     3241 ccagcgttcc ccagatggtc cgacccttta cggatgggct gctgtaatga atggttatcg
     3301 tggcgccatt gacggtaccc gataccgact gggccgggct tacccgtggc tttgcggttt
     3361 gggctgatcc ggtgagggtg cataagaggg tgagccccgt aaacaataat tgttgaatgt
     3421 gtttcatgtt tttttttaca caaaacaaca ccggatggac ccctccaaaa aatagattca
     3481 acctacgccg taaaaagcta atcaatcccc ctgccggccg gccattgagc gaaacagggt
     3541 gtttgttgaa cccatccggg tggtaagccg gaaaacaggt attttgcggc tgcaatgcgt
     3601 aaaaggatgt cgtttacggg caagtccact ctcaccgggc tgcaatatct cgtgtgggcg
     3661 gcaatactgc tgatcggttt tttcgccatg gtatccagcg atggcgtaaa gcgatccgcg
     3721 gcattcgccg ttaacaatac ctgtttttac gcgctgatcg tctatggcaa tatccgcttc
     3781 ctctaccccc ggttgtacca gaagaaacgc tatgtgccgt atgtcgcggg gtcggtgctc
     3841 ttgttaatgc tggccggcgc tggcaggggg tattgttcgt tgatcgtgcg gaacgaatat
     3901 tccctcgctt cgtcgtggtg gcgggacccg gccacctggc tcaccttctt cctgtcgggc
     3961 ataaccgtct ttatactcag ctttatcttc cggctggcca tcgcgtattt tttggtcaaa
     4021 caggcctccg ag
//
LOCUS       DATGFG010000965         4719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1360712, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000965 DATGFG010000000
VERSION     DATGFG010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360712"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..2468)
                     /locus_tag="VKQ52_18185"
     CDS             complement(27..2468)
                     /locus_tag="VKQ52_18185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89194.1"
                     /translation="MLKTYFKVAFRSLVRNKVFTIINIFGLAIGLTTCLLILLYIVSE
                     SGYDRQNKDADLIYRVVQTANTASGNLEKGWAGTPAPLAGTLKTDFPEVAVTTRLLKS
                     PITDKYLLTTTNGKEKKQFYETNGYYADSTFFQVFTYDFRYGDASTALFRPNSIVLSE
                     ELAKKLFGDENPVGRLITLGLSFGNFDYMVGGVFLDKDRKSHIPAHFFLSMNNNDFGK
                     WVPTETNWASSSIFYTYIRLKATPDLAAFQRKVNTDIDKYAAADLKAFGVSRRLFLQP
                     VTSIYLHSHVDYEVATVGNATTLYILGSIAVFVLLIACINFMNLSTARSAKRAKEVGV
                     RKVLGAEKHSLVVQFLGESILMSLMALLLAIGFTRLLLPAFGNLVQKQLHLFDEPILW
                     VGIGALTLATGIIAGLYPAFYLSAFRPIAVLKGKLLNNFSALTIRKGLIVFQFTVSIC
                     LVSGAIIIARQLDFLDNRPLGFKKDQQLIVPLQNKDAGDHFTALKDELTKLPAVRAVT
                     GGSSYPGLANINGMLFYGEGKTVQDVIDISLATPEEDFLKTLGLTLLQGREFTKASTA
                     DSNSIILNETALRQLNYEPKTAIGRKISYDLQGQHGTLQIVGVIKDFNFESLYRPIKA
                     FGLAYVFGGARRSYLIASLSAGNYAPVLRDVAAAWKRINPGSPFTYSFLDKDFQANYE
                     KDQQASRIVGYFTIVTILIACLGLFGLSVFSAEQRAREIGIRKVLGASVTDITLLLSR
                     DFLGLVCIAILISSPLAWYGMNRWLQGFAYRIPIGWWLFPVAGLIAVGIAWLTVSFQA
                     IKTALTNPVKALRSE"
     gene            2784..3554
                     /locus_tag="VKQ52_18190"
     CDS             2784..3554
                     /locus_tag="VKQ52_18190"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLZ89195.1"
                     /translation="MLKSKVPFVCSLAFVLALPSAANPGRPALDKPKAVVVPASFTDE
                     KTVSFALPGQDEMAFVNAYIRENDESLLAVKHRSKRPFKIIEAVLQKFGLPAELKYLA
                     VIESDLKTTAVSRVGAKGPWQLMASTAQDLGLKVDSVDDERTNIYKSTKAAALYLRDL
                     HRAFKDWRLVLAAYNAGPLSVYRAMRRSNSTDFRVLERYLPRESRQHVKRFMATAYYF
                     DGEVSQNERVTQIAKTAQISQGAKTAQVSPSVPSSRYS"
     gene            complement(3520..4221)
                     /locus_tag="VKQ52_18195"
     CDS             complement(3520..4221)
                     /locus_tag="VKQ52_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ89196.1"
                     /translation="MDDEPLAREVIGRFIARVPTLQLMAECENAIQAMTVIQQQDIEL
                     IFVDIQMPELLGTEFIKILQHPPKMILTTAYPEYAVESYDLDVVDYLLKPIQFERFLK
                     AVNKATLQIGGTGQAQTQLQPSAQKEEPPESYLYFRTDRKMVKVMLDEILYIEGMKNY
                     IKIITGHGTIITKNSMAAVEAMLPEAAFIRTHRSYIVSRTKIRSFTGESIELGNVEIP
                     IGKLFKNSVMKALTG"
     gene            complement(4250..>4719)
                     /locus_tag="VKQ52_18200"
     CDS             complement(4250..>4719)
                     /locus_tag="VKQ52_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89197.1"
                     /translation="GLLRYILYDCDRPLVKLSQEFRLIGDYLDLEKVRYTNLDLRTQL
                     PADGDRYTIAPLLLLPLVENCFKHGTSRMIEQPWISIDADLKQNWLWVKLINGRPAVE
                     TPDNFTEGIGLSNVRKRLQLLYPDKHELNIISEQDVFIVNFKIELQEDRTQNG"
BASE COUNT         1018 a         1123 c         1461 g         1117 t
ORIGIN      
        1 ctcaatgggg atctgtacag gtaagtttat tcgctacgca aggcttttac ggggttggtg
       61 agggctgttt tgatcgcctg gaagctgacg gtcagccacg caatgcccac cgcgatgagg
      121 cccgcgacgg ggaacagcca ccaaccgatc gggatacgat aggcaaagcc ctggagccac
      181 cggttcatcc cataccaggc caacggcgaa gaaatgagaa tggcgatgca aacgaggccg
      241 aggaagtcac gggagagcag cagggtgata tcggtcaccg aagcgcccag tactttgcgg
      301 atgccgatct ccctcgcccg ttgttcggcg gaaaagacgg agaggccgaa gaggccgagg
      361 caggcgatga ggatggtgac gatcgtaaaa tagccgacga tgcgggaggc ctgctggtct
      421 ttttcataat tggcctggaa gtccttatcg aggaaggagt aggtaaatgg ggagccgggg
      481 ttgatgcgtt tccatgcggc tgccacgtct cgcaggacgg gggcgtaatt gcccgcgctg
      541 aggctggcga tgagatagct gcgccgtgcg ccgccgaaga cgtaggcgag gccgaaggct
      601 tttatcggcc ggtagaggct ttcgaagttg aagtctttga tcaccccgac gatctgcagg
      661 gtgccatgtt gtccctgaag atcgtagctg atctttctgc cgatggcagt tttcggttcg
      721 tagttcaatt gtctcagcgc tgtctcgttg aggatgatgc tgttggaatc cgccgtggat
      781 gcttttgtga attcgcggcc ctggagcagg gtcagaccca gggtctttaa aaagtcctct
      841 tcgggggtcg ccaggctgat atcgatcacg tcttggacgg tcttgccttc gccatagaaa
      901 agcatgccgt tgatattggc gaggccggga taggatgatc cccccgtgac ggcgcggacg
      961 gcggggagct tggtgagttc gtccttcaat gccgtaaagt ggtctccggc gtccttgttt
     1021 tgcagaggga cgatcaactg ctggtctttt ttgaagccga gggggcggtt gtccaggaag
     1081 tcgagctgcc gggcgatgat gatcgcgccc gagaccagac agatggagac cgtaaactgg
     1141 aagacgatga ggcctttgcg gatggtcagg gcggagaaat tattgagcag ctttcctttc
     1201 agtacggcga tgggccggaa ggcggaaaga tagaaggcag ggtagagccc ggcgatgatg
     1261 ccggtggcga gggtaagcgc cccgataccc acccagagga tgggttcatc aaagaggtgc
     1321 agttgttttt gtaccaggtt gccgaaggca ggcaggagca ggcgggtgaa gccgatcgcg
     1381 agcaggaggg ccatcaggct catcaggatc gattcgccga ggaattggac gacgagcgaa
     1441 tgtttttccg cgccaaggac cttgcggaca cctacttctt ttgccctttt ggcggaccgg
     1501 gccgtcgaga ggttcataaa gttgatacag gcgataagca acacgaagac ggcgatgcta
     1561 cccaggatat agagcgtggt ggcattgccg acagtcgcca cttcgtaatc gacgtgcgaa
     1621 tgcagataga tgcttgtgac aggctggaga aagaggcggc gcgagacccc gaaggccttc
     1681 aggtcggccg cggcatattt gtcgatgtcg gtgttgacct tgcgttggaa ggcggcgaga
     1741 tcgggggttg ctttcagccg gatataggta tagaagatac ttgaggaggc ccagttggtt
     1801 tccgtcggca cccatttgcc aaaatcgttg ttgttcatgg aaaggaagaa atgagcgggg
     1861 atatgggatt tgcgatcctt gtcgagaaaa acgcccccta ccatatagtc gaagttgccg
     1921 aaggaaaggc cgagggtgat caggcggccg accgggttct catctccaaa gagttttttg
     1981 gccagttctt ccgaaagaac gatgctgttt ggccggaaga gggcggttga tgcatccccg
     2041 tagcggaagt cataggtgaa gacctggaag aaggtagagt cggcgtagta gccgtttgtt
     2101 tcatagaact gcttcttttc cttcccgttc gtggttgtga gcaggtactt atcggtgatg
     2161 ggagatttca gcaggcgggt agtgacggcc acttcgggaa aatcggtctt cagggttccg
     2221 gcaagaggtg cgggcgtacc ggcccagcct ttttccaggt tacctgaggc agtgttggcg
     2281 gtctggacga cgcggtagat aagatcggcg tctttgtttt gccggtcata accggattcg
     2341 ctgacgatat agaggaggat caacaggcag gtggtgaggc cgatggccag gccaaagata
     2401 ttgataatag taaaaacctt gttgcggaca aggcttcgga aggcgacctt gaagtaagtt
     2461 tttagcatga taagcgagag accaaaaata tgccattggt ataattgact ggcgatcagt
     2521 tccttgatat ttgggtgtgt ctgacaggcg tccggatgtg atacagcgat tgtccgatgt
     2581 cgtggtttta ccacgtaaaa gcacctatga ggcgcggcga atggatatta tatttgtctt
     2641 tcagttatcg ccaccgtgaa tgaaaaaccc attcaccgta cccacggcgg gcaatcaact
     2701 cctccactat tctattctta accctcacac gcaatcagta atccatacct gggaccggca
     2761 ttgttttact caaacttaaa acgatgttga agtctaaagt accctttgtt tgcagcttag
     2821 cctttgtgct ggcccttcct tcggctgcaa accccggtcg acccgctctt gataaaccga
     2881 aagccgtggt tgtccctgct tcatttaccg atgaaaagac agtttctttt gccctgccag
     2941 gtcaggatga aatggcgttt gtgaatgcct atatccggga gaacgatgag tcgctcctgg
     3001 cggtgaagca taggagtaaa cgacctttta agatcatcga agccgtattg cagaagttcg
     3061 gcctgccggc ggagttgaag tatctcgccg tgatcgagtc tgacctgaaa acgactgccg
     3121 tctcgagggt cggggcgaaa ggtccctggc aactgatggc gtccaccgcc caggatctgg
     3181 ggctgaaggt cgatagcgtg gacgacgagc ggacgaatat ttataagagc accaaggcgg
     3241 ccgctttata cctgcgggac ctccaccggg cattcaagga ctggcggctt gtgctggcag
     3301 cttataatgc cgggccgtta tcggtgtacc gggcgatgcg ccgcagcaac agtaccgatt
     3361 tccgggtgct ggagcgctac ctgccaagag aatcgagaca gcatgtgaag cggttcatgg
     3421 ccacggccta ttattttgat ggggaagtga gccagaacga gcgagtgacc cagatcgcga
     3481 agactgcgca gattagccag ggtgcaaaga cggcgcaggt tagcccgtca gtgccttcat
     3541 cacgctattc ttaaacagtt tgccgatggg gatctcgaca ttgccgagtt cgatggattc
     3601 gccggtgaag gacctgatct ttgtccttga cacgatataa gagcgatggg tccggatgaa
     3661 ggcggcctcg ggcagcatgg cctctaccgc cgccatcgag ttctttgtga tgatggtccc
     3721 gtgacccgtg atgatcttga tatagttctt catcccttcg atgtacagga tctcatcgag
     3781 catgaccttt accattttcc ggtcggttcg aaagtagaga taggactccg gtggttcttc
     3841 cttttgggcg gatggctgga gttgggtttg ggcctgcccg gttcccccga tctgcagggt
     3901 ggccttgttc accgccttta gaaaccgttc gaactggatg ggctttagca gatagtccac
     3961 gacatccagg tcatagctct cgaccgcgta ttccgggtag gccgtggtga ggatcatctt
     4021 tggcggatgc tgcaggatct ttatgaattc cgtgcccagg agctccggca tctggatatc
     4081 cacaaatatc agctcgatgt cctgttgctg gatgacggtc atcgcctgga tggcgttctc
     4141 gcattccgcc atcagctgca gggttggcac ccgggcgata aagcggccaa tgacctctct
     4201 tgccaggggt tcgtcgtcca cgaccatgca ttttatcttt tttatcgtcc tatccattct
     4261 gcgttctatc ctcctgtaat tctatcttaa aatttacgat aaaaacgtct tgttccgaaa
     4321 tgatgttcag ctcatgtttg tccgggtaga gtagttgcag ccgctttctg acattgctca
     4381 ggccgatgcc ttcggtaaag ttatccggcg tttcgacggc gggcctgccg ttgatgagtt
     4441 ttacccatag ccagttctgt ttcagatcgg cgtcgatgct gatccagggt tgttcgatca
     4501 tccggctggt gccgtgtttg aaacagttct ccacgagggg gagcagtaac aggggggcga
     4561 tcgtatagcg atcgccgtcg gcgggcaatt gggtgcggag atcgagattc gtatagcgga
     4621 ccttttcgag atcgaggtaa tctccgatga ggcggaactc ctgggatagc tttacgaggg
     4681 gccggtcgca gtcgtagagg atgtaacgca ggagtccgg
//
LOCUS       DATGFG010000966         4851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1193235, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000966 DATGFG010000000
VERSION     DATGFG010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193235"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VKQ52_18205"
     CDS             complement(<1..206)
                     /locus_tag="VKQ52_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89198.1"
                     /translation="MRSIKRWWMSMVYAGAQDLPSHDPQRRVIVGINLMSLSIGILNL
                     SIGTFVGIIIGKTDILAGVAVETL"
     gene            complement(440..1621)
                     /locus_tag="VKQ52_18210"
     CDS             complement(440..1621)
                     /locus_tag="VKQ52_18210"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLZ89199.1"
                     /translation="MKFRLFIAVCLILTCSCQSKKKGIFSVSGTFRNADKLSAVEGPI
                     SKVYLMEVAYGKDQPPVVLDSAKLPAGNGSFSLSGITHNQEIFELVFGNNAIAVPLIN
                     DATDVRVNVDLGKKDDFYDVSGSDASSQLKELITIFGKKNFEVDRSMSELDSLKTVNA
                     PDSLLITATNKKNNAIQDLNTYLKQFLNSNTNATICALALSWSSRSFSKSEFEAALGD
                     LLRKYPDNTVLNGLKQNYDQQLAQMAEQERKDHENSWVGKPAPDLALPDASGRIISLA
                     SYKGKYLLVDFWASWCAPCRAENPNVVKAYNEFKGKNFAILGVSLDKEKDSWQQAIRA
                     DRLDWTHVSDLKYWNSKAVETFKFNGIPYNVLIDPQGKVIAESLRGEDLESKLREVLT
                     N"
     gene            complement(1627..3078)
                     /gene="gatB"
                     /locus_tag="VKQ52_18215"
     CDS             complement(1627..3078)
                     /gene="gatB"
                     /locus_tag="VKQ52_18215"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLZ89200.1"
                     /translation="MAINYDAYETVIGLEVHAQLLTQTKLFCGDSAAFGGEPNTHVSP
                     ITLALPGSLPRLNRKAVEYAIKMGLTCHCEIEKNNYFARKNYFYPDLPKGYQTSQHTT
                     PICKGGWVRIRTAAGERDIQLNRIHLEEDAGKSIHDAVPDATALDLNRAGVPLIEIVT
                     EPDLRSGDEAYAYLTELRKTLRYLQICDGNMEEGSMRCDANVSVRLKGETTLGTKVEV
                     KNLNSIRNVRRAIEIESRRLIELVATGQPVVQETRSFDATDGSTFPLRTKEEANDYRY
                     FTDPDLPPFQVTDAFLESIKASIPPLPEELIQKYTRELQLPEYDARLLCDDRDSAAYF
                     EQLISHTANCKAAANWLLGPVRSGLNDKGLDWKDLPVRPESLARLVRLVEEGRLSFSI
                     AAERIFPVLMAEPQRDPQEIATSLNLVQSSDTSEITAWIEQALAGMPDKVSEYKKGKK
                     GLIGLFVGEVKKLSKGKADPKLTNKLLLEKLEN"
     gene            complement(3176..4243)
                     /locus_tag="VKQ52_18220"
     CDS             complement(3176..4243)
                     /locus_tag="VKQ52_18220"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLZ89201.1"
                     /translation="MNKQLLKIAGLAALVLSLHSQGFSQNAGSSESEDNDTTPVRSRS
                     WDEITIRHKGDKDARVTVEIKNGEIFINGKPASEYKDDNLTITKRKFKTVGDDDVLFS
                     DGDGAMTITPFRNNGGGWNYDRRITPRKGVSGAFLGVTSLKPENGPDGAKVGEVSEGS
                     AAEKAGLKEGDLITRVDDKTIDGPESLSKTIRGYKPGDKVTITFKRDNKEQKVTAELG
                     KSRTDNIELYSFDGRGMNFDAPEFRMPKMYMPRNFNFSYGDKQLRLGIHAQDTEDGKG
                     VKVLDVDDESAASKAGIKEGDIITKFDGQEVNSASKLAELARVSREDKSKPAVKVSLL
                     RGGKPLEVEVKTPRKLRTADL"
     gene            complement(4361..4597)
                     /locus_tag="VKQ52_18225"
     CDS             complement(4361..4597)
                     /locus_tag="VKQ52_18225"
                     /inference="COORDINATES: protein motif:HMM:NF024251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HLZ89202.1"
                     /translation="MMNELPPQPDPELLTALVTMKMPFGKYKDTVLCHLPVSYLEWFH
                     REGWPKGKLGMLLATIYEIKINGLEKLLDPLKKR"
     gene            complement(4602..>4851)
                     /locus_tag="VKQ52_18230"
     CDS             complement(4602..>4851)
                     /locus_tag="VKQ52_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89203.1"
                     /translation="GTLPFLAVLAANIEGKAAVALLVDEQLVAARGWQAPAIIKERVA
                     PLIKGGGGGQKTLATAGGQDVGHLGEVIGKVKDLLTGN"
BASE COUNT          957 a         1337 c         1304 g         1253 t
ORIGIN      
        1 atcagggtct ctactgcgac acccgcgagg atatctgttt ttccgatgat gatccctaca
       61 aatgttccga tgctgagatt gaggatgcct atcgagagcg acatcaggtt tattccaacg
      121 atcactcgcc gctgtggatc atggctgggc aggtcctggg caccggcata gaccatcgac
      181 atccaccaac gttttatact tctcatagac cgcatttact atgagcttaa tacgttaaaa
      241 atacagtttc taaccaagaa aggataaaac tggataaaac ccgggggaaa acccttacgt
      301 agagaaatct ctacaaaggg agatggtttt tggtcctaat tttcaaacct aataggataa
      361 ggtttaatgg ttctggtatt tgattagtaa aaaaaagacc cgccaaatgg cgggtccttt
      421 ttattttgtt taccatcaat tagttagtaa gaacttccct gagcttgctc tcgaggtcct
      481 ctccccggag gctctctgca atgacctttc cctgcggatc gatcagtaca ttatagggga
      541 tcccattgaa cttaaaggtc tcgacggcct tgctgttcca gtatttgagg tcgctgacat
      601 gcgtccaatc cagccggtcc gcccggatcg cctgctgcca ggaatccttt tccttgtcaa
      661 gggatacgcc caggatggcg aaattcttgc ctttgaattc attatatgcc ttgacgacat
      721 tggggttctc ggcccggcag ggagcgcacc agcttgccca gaaatcgacg agcaaatatt
      781 ttcccttata ggaagcaagg gaaatgatcc tgccgctggc atcgggaaga gcaagatccg
      841 gcgccggttt cccaacccaa ctgttctcgt ggtccttacg ttcctgctcg gccatctgcg
      901 ccagttgctg atcgtagttt tgttttaagc cgttcagcac cgtattgtcc gggtatttcc
      961 gcagcagatc gccaagggcg gcttcgaatt ccgatttgct gaagctgcgg gaagaccagc
     1021 tcagcgcaag agcgcagatc gttgcgttgg tgttgctgtt gaggaattgt ttcaaatagg
     1081 tgttgaggtc ctggatggca ttgttctttt tattcgtggc ggtgatgagc aggctgtcgg
     1141 gcgcattgac ggtcttgagg ctgtcgagtt cgctcatcga cctgtccacc tcgaaattct
     1201 ttttgccaaa gatggtgatc agttctttca gctggctgct ggcatcggag ccgctcacat
     1261 cataaaaatc gtccttcttg ccaagatcga cattgacccg cacgtcggtg gcgtcgttga
     1321 tcagcgggac agcgatggcg ttgttgccaa agacgagttc gaatatctcc tggttatggg
     1381 tgatccccga taggctaaaa ctgccattgc cggcgggcag cttcgcggaa tcgagcacca
     1441 ccggtggctg gtctttccca taggcgactt ccatcaggta gaccttggag atcgggcctt
     1501 cgacggcgga aagcttatcc gcattcctga aggtcccgga tacggagaaa attcccttct
     1561 tctttgactg acagctacag gtcaggatca ggcagaccgc gataaataat ctaaacttca
     1621 tatcccttaa ttttccagtt tttcgagtaa taatttattg gtgagtttag ggtccgcttt
     1681 ccccttcgac aacttcttta cttcgcccac aaagagcccg atgagcccct tctttccctt
     1741 cttatattcc gatactttgt cgggcatccc ggccagggcc tgttctatcc acgccgtgat
     1801 ctccgatgtg tcggaactct gtaccaggtt gagggaagtg gcgatctcct gcggatcgcg
     1861 ctggggttcg gccatcagga ccgggaagat cctttcggcc gcgatggaaa agctgagcct
     1921 tccctcctcc acgagccgga caagcctcgc cagcgattcc ggccggacgg gaaggtcctt
     1981 ccagtccagc cccttgtcgt tgagtcccga tcggacagga cccaacagcc agttggcggc
     2041 ggccttgcaa ttggcggtat ggctgatcaa ctgttcaaaa taggcggcgc tgtcccggtc
     2101 atcacaaagc aggcgggcgt cgtattccgg cagttgtaat tcgcgggtat atttttggat
     2161 cagctcctcc ggcaacggag ggatcgaggc ctttatcgat tcgaggaagg catccgttac
     2221 ctggaagggc gggaggtccg gatcggtgaa gtaccggtaa tcgttggctt cttcctttgt
     2281 gcgcaggggg aacgtgctgc cgtcggtggc gtcaaagctc ctggtctcct gcactaccgg
     2341 ctgacccgtc gccaccagtt cgataagccg ccgggattcg atctcgatgg cgcgccggac
     2401 attccggatg gagttcaggt tctttacttc gaccttggta ccgagcgtcg tctctccttt
     2461 cagccggacg gagacattgg cgtcgcaccg catgctgcct tcttccatat tcccgtcaca
     2521 gatctgcagg tagcgtaggg tcttgcgcag ttcggtaaga taggcatagg cctcgtcccc
     2581 gctgcggaga tcgggttcgg tcacgatctc gatcagcggt acgcccgccc ggttaagatc
     2641 cagggccgtc gcatcgggga cggcatcgtg gatgcttttg ccggcatctt cttcgaggtg
     2701 gatgcggttg agctggatat cgcgctcgcc cgcggcggtc cgtatccgga cccagcctcc
     2761 tttacagatc ggggtcgtat gctgtgaggt ctggtagccc tttggcagat cgggatagaa
     2821 ataattctta cgggcaaaat agttgttctt ctcgatctca cagtgacagg tcagccccat
     2881 cttgatcgca tattccactg ccttccggtt caacctgggc agggagcccg gcagggcgag
     2941 cgtgatcggg ctgacatggg tgttgggctc gccgccaaat gccgcggagt cgccgcaaaa
     3001 taatttggtt tgggtaagca gttgcgcgtg cacttccagc ccgattaccg tctcgtatgc
     3061 atcataattg attgccatgt gaatatgtcg gccacctggg ccatcgacag gcatcaaaag
     3121 tacgaaaaaa aggcgtcccg ccggggacgc cctgtatagg aagttcatgc atcgatcaaa
     3181 gatcggccgt ccggagtttt ctcggtgtct ttacctcaac ctcgaggggt ttgccgcccc
     3241 ggagcaggct tacctttacc gcgggtttgg atttatcctc ccggcttacc cgggccagct
     3301 cggccagttt tgaggcgctg ttcacctcct gcccgtcgaa tttggtgatg atatcccctt
     3361 ccttgatacc cgccttcgat gcagcagatt cgtcatcgac gtccagcacc ttcacccctt
     3421 tgccgtcttc ggtgtcctgg gcatggatcc ccagccgtag ctgtttatcg ccatagctga
     3481 aattaaagtt cctgggcata tacattttcg gcatccggaa ctcgggggcg tcgaaattca
     3541 ttcccctgcc gtcaaaactg taaagctcga tgttatcggt ccgggacttc cccaattctg
     3601 ccgtcacctt ctgttctttg ttgtcccttt tgaaggtgat ggtcacctta tccccgggct
     3661 tatagccacg gatggtcttc gagagggact cgggtccgtc gatcgtcttg tcgtcgaccc
     3721 tggtgatcag gtcgccttct ttcagcccgg ccttttcggc tgcgctgccc tcggatacct
     3781 cgcccacctt ggcgccatcg ggaccattct ccggttttag cgaggtcacc ccgagaaacg
     3841 cgccactaac gcccttgcgc ggggttattc tccgatcata gttccagccg ccgccattgt
     3901 tgcgaaacgg cgtgatggtc atcgctccat cgccgtcgga aaataaaacg tcgtcatcgc
     3961 ccacggtctt gaacttccgt ttggtgatcg tgaggttatc atctttgtat tccgaggccg
     4021 gtttgccatt gatgaatatc tcaccgttct ttatctctac cgtcaccctg gcatccttgt
     4081 cccccttgtg ccggatggtg atctcgtccc aggaacggct gcgcaccggg gtagtgtcat
     4141 tgtcctccga ctcggaagag ccggcgttct gggaaaaccc ctggctatgc agcgacagga
     4201 ccagggcagc gagtcccgcg atctttagga gctgtttgtt catcacatca gttttttgaa
     4261 attaaggaat aaatttatct acgtcgtttt tcgtagatca aatatagggg gatttcccga
     4321 aatacgaaag ttttcggaaa tgttaaagtt agcggcgcgc tcagcgcttt tttaaggggt
     4381 cgaggagttt ttccagtccg ttgatcttga tctcgtaaat ggtggccagc agcatcccca
     4441 atttcccctt gggccagccc tcgcggtgaa accattccag gtaggatacg gggagatggc
     4501 aaaggacggt atccttgtat ttgccaaagg gcattttcat cgtcaccaat gccgtgagca
     4561 gttcggggtc gggttgtggc ggaagctcgt tcatcatctt attaattgcc tgtcaataag
     4621 tcttttactt tcccgatcac ttcccccaaa tgccccacat cctgcccccc ggccgtggcc
     4681 agcgtcttct gaccgccgcc gccgcccttg atgaggggag ctacccgttc cttgatgatc
     4741 gcaggcgcct gccatccgcg ggcggcaacc aattgttcgt ccaccagcag ggccacggcg
     4801 gccttgcctt cgatattggc ggcaagcacc gcgaggaagg ggagcgtgcc g
//
LOCUS       DATGFG010000967         4257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_395637, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000967 DATGFG010000000
VERSION     DATGFG010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395637"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1893
                     /locus_tag="VKQ52_18235"
     CDS             175..1893
                     /locus_tag="VKQ52_18235"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HLZ89204.1"
                     /translation="MDDMHSVDLATDDQHSVGPTTDGKHSVGLTTHDNHRVDQTTDDM
                     TTLRTELERLETRLHSAETVNREVYASLHPGQREAARNLFGYLCLRSEDLRDMQVRLH
                     NAGLSSLASSESHILRQVESILERLGKVYPPDKLSIRNYAFSRESIGRRSALLFGAKQ
                     DAVIPYLMITFDAAFAEDHAFISTLLLHGMNVARINCAHDDAATWKKMIGSIRQATEA
                     TGLTCNIYMDLAGPKIRSVIRGKGKSHGKEKDHEKKNHEMKNHENEKNDETERNHEKV
                     KNHGKMKIAEGEELILAEEGADLHRKGLPKKDLHKKEKVIGCSLAGIVRHLEKGHRVL
                     IDDGVIEAVVEEPGEERARLRITRVSAEKHTLKAEKGMNFPDTVFVVPALTDYDRECL
                     PFIREYADIVGYSFVEDEAALAELQGLLSAGEGREPFIVLKIERAAAVKNLPGLLLQG
                     MRKEAFGIMIARGDLAVEIGFERLSEIQEEILWLSEAAHVPVIWATQVLETLNKTGLA
                     TRSEVTDAAYAAQSECVMINKGEHLLKVIDVLKDILLRSGGHHLKKRYTFRPLSIARN
                     FMHPDE"
     gene            complement(2175..3425)
                     /locus_tag="VKQ52_18240"
     CDS             complement(2175..3425)
                     /locus_tag="VKQ52_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89205.1"
                     /translation="MKPALTAVATLALFALACNKHNSNPPQPSPSDSLRTYNLAFSYP
                     DSSRTPAADWELIVTEPGGKQLLDTIVPVNTPITATIHTTHSLVNLGSVYRAPTDTSA
                     TVRIITGIDPATITSPVPNFVTSPQLLGPTVNRAIYYQNIPANDINHRIYYSQDILPV
                     NFDINSFGHMPRRVGNYYLINYASKGLYHFYPWRDGSATDTFDLALLTDTSTQLNLTT
                     HAGYGRPLCWVVGIMDTTDYRKSVFLYNYIYNIYQTVSADIVYPTKNVQKFELRGTYI
                     GNDSTEVTIYTYGNTVTADHPFMDASIFTLLSRQQDSFAVKFSTTQLSYYGTYWKIPG
                     LDYYTYSSPDSPVSHPLALINSLKSRKLQSQSLSPLTHLTPGWLLMQTTPGLNYADYM
                     SYVCNVPLWKARRVASATQLVRKF"
     gene            complement(3457..>4257)
                     /locus_tag="VKQ52_18245"
     CDS             complement(3457..>4257)
                     /locus_tag="VKQ52_18245"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLZ89206.1"
                     /translation="NAKIRRGPGIDDKPVPLKKQNTTMEGNILAIDIGGSHIKGTILN
                     TKGEQQTDYKKFITPAHVTAAGVIDIIQQLAKDMPGYEKVSVGFPGYVRNGVVYTAPN
                     LFTASWKEVPLGQEIADALKRPVRLVNDADQLGLGVVSGKGFELAVTLGTGFGTAVLI
                     DGYLLPHLELAHHPISKGRDYDVYIGAKALEEEGKKKWNQRMTKVLKILKVVFNYDRL
                     YIGGGSVGKLDVAPDPNIHIFSNLDGIKGGAKLWELEDRYHITTNHPS"
BASE COUNT          944 a          927 c         1451 g          935 t
ORIGIN      
        1 cgtggcggcg cgggggcagg tgaggtgcag agcgggcgag atgcgaagcg gatgatgcgg
       61 ccggaaaacc attcggggct gagccagcga aaattaatgg catgataata tcacgcaata
      121 gtctttgtca taaattgagc cgacgctgaa gacataaaca gcgttgccct gacaatggac
      181 gacatgcaca gcgtcgacct ggcgacggat gaccagcaca gcgttggccc gacgacggat
      241 ggcaagcaca gcgttggcct gacgacacac gacaaccatc gcgttgacca gacaacagac
      301 gatatgacaa cgctacggac agagctcgag cggttggaaa cccggctgca ttccgccgaa
      361 acggtcaaca gggaggtgta tgcatccctt catccgggcc aacgggaggc tgcgcgaaac
      421 ctgttcgggt atctttgttt gcgaagcgag gacctgcggg atatgcaggt aaggttacat
      481 aatgccgggc tttcgtccct tgccagcagc gagagccata tccttcggca ggtggaatcc
      541 atcctggaac ggttaggaaa ggtttaccca ccggataaac tcagtatccg caactatgct
      601 ttcagccgtg aatccatcgg caggaggtcg gcgctgctct tcggagcgaa gcaggatgcc
      661 gtcatcccct acttaatgat caccttcgat gctgctttcg cggaggatca cgccttcata
      721 tccacattgc tattgcacgg aatgaatgtg gcgaggataa attgcgcgca cgatgatgcc
      781 gccacctgga aaaagatgat cggatcgatt cgtcaggcta ccgaggccac gggtctgacc
      841 tgtaatatct atatggatct cgcgggtccc aagatcagat cggtcatccg gggaaaaggg
      901 aagagccatg ggaaggagaa ggaccatgag aagaagaatc atgagatgaa gaaccatgag
      961 aacgagaaga acgatgagac ggagagaaac catgagaagg tgaagaacca tggaaagatg
     1021 aagatagccg aaggggagga actgatcctg gcggaagagg gggcggacct tcataggaag
     1081 ggccttccta aaaaggatct tcataaaaag gaaaaggtca ttggttgttc gctggccggg
     1141 attgtgcgtc acctggaaaa ggggcatcgt gtcctgatcg atgacggggt gatcgaagcc
     1201 gttgtcgaag agccgggaga ggaaagggcc agactgcgca ttacgcgggt atcggccgaa
     1261 aaacatacgc taaaggcgga aaagggaatg aacttccccg acacggtatt tgttgtcccg
     1321 gcattgacgg actacgaccg cgagtgtctg cccttcatcc gcgaatatgc tgatattgtc
     1381 ggctattcgt ttgtagaaga tgaggctgcg ctggcagagc tccagggctt gctgagcgcc
     1441 ggcgaaggcc gtgagccctt tatcgtcctg aagatcgaga gggccgcggc ggtgaagaac
     1501 ctgcccggcc tgcttttgca gggcatgcgg aaagaggcgt tcggcatcat gatcgcgcgg
     1561 ggtgatcttg cggtggagat cggttttgaa cggctcagcg agatacaaga agagatactc
     1621 tggttgtcgg aggcggcgca tgtgcccgtg atctgggcaa cgcaggtgtt ggagaccctc
     1681 aataaaaccg gtctggccac cagatcagag gtgactgacg cggcttatgc cgcccaaagc
     1741 gagtgcgtga tgatcaacaa gggcgagcac ctcctaaagg taatagacgt gttgaaagat
     1801 attttgctgc gcagcggcgg tcatcattta aagaaacgct atactttccg gccactgagt
     1861 atcgcccgga attttatgca cccggatgaa tagtgtgggg gcagggtgtt acccggaggc
     1921 aaagtgttgc ccgtgggcaa agtgttaccc gggggcaggg tgttgcccgg ggacaaagtg
     1981 ttacccggag gcaaagtgtt gcccgtgggc aaagtgttgc ccggggggca gggtgttgcc
     2041 cggaggcagg gtgttagtac ccgtgcggat aggcggtagc gagagtgttt ttgccaggcc
     2101 gggggccgtg gggagatcgg cgggggccgt ggggagatcg ggtgggtgtc ggtgggagga
     2161 tcgggcgggg gccgtcaaaa ttttcggacc agttgagtgg cggaggctac ccgtcttgct
     2221 ttccacagcg gaacattaca gacataagac atatagtccg cataattcaa cccaggagtg
     2281 gtctgcatca ggagccagcc cggtgtcaga tgtgtcagcg ggctcagcga ctggctctgc
     2341 agtttccggc tcttcaggga attgatcagg gccaggggat ggcttaccgg tgaatcgggc
     2401 gaggaatagg tataataatc caggccgggg atcttccagt atgttccgta ataacttaat
     2461 tgcgtggtgg aaaatttcac cgcgaagctg tcctgttgac ggctgagaag ggtgaagatg
     2521 gatgcatcca taaagggatg atccgctgtg accgtgttac cataggtgta gatggtgacc
     2581 tcggtggagt cattgccgat ataggtgccg cgcaattcga atttctgtac atttttcgtt
     2641 gggtagacaa tgtcggcgga gacggtctga tagatattgt aaatataatt atagagaaag
     2701 acgctttttc tgtagtccgt ggtgtccatg atccccacca cccagcaaag gggtcgcccg
     2761 tatccggcat gcgtagtgag attcagttgc gtggaggtgt cggtgagcag ggcgagatcg
     2821 aaggtatccg tggcggagcc gtcgcgccag ggatagaagt gatagaggcc tttcgaggcg
     2881 tagttgatca ggtaatagtt gccgacccgc cggggcatat ggccaaagct gttgatgtca
     2941 aagttgaccg gaaggatatc ctggctgtaa taaatacgat ggttgatatc gttggccggg
     3001 atattctggt aatagatggc acgattgacg gtggggccga gaagttgtgg tgaagtgacg
     3061 aaattcggca cgggggaggt gatggtggca gggtcgatac cggtgatgat cctgacggtt
     3121 gcggaggtgt cggtgggtgc gcggtataca ctgccgagat tgaccaggct gtgggtggta
     3181 tggatcgtgg cggtgatggg ggtattcacg gggacgatgg tgtcgagcag ttgtttgccg
     3241 ccgggttcgg tgacgatgag ttcccaatcg gcggccggag tgcggctgct gtcgggatac
     3301 gaaaaggcca gattatacgt gcgcagggaa tcggatgggg agggctgcgg agggttggag
     3361 ttgtgcttgt tacacgcaag ggcgaagaga gccagggtcg caacggcggt gagggcgggt
     3421 ttcatactga caggtttgag taggcaaata tagggatcag gaggggtggt tggtagtgat
     3481 atgatatcta tcctcgagtt cccagagttt tgcgccgcct tttatcccgt cgagattgga
     3541 gaagatatga atgttggggt cgggggcgac gtcgagtttg ccgacactgc cgccgccgat
     3601 atagaggcga tcgtaattga atacgacttt gaggatcttc aggacctttg tcatccgttg
     3661 attccatttt ttctttcctt cttcctcgag ggcttttgcg ccgatgtaga cgtcgtagtc
     3721 gcggcccttg ctgatggggt gatgggcgag ttcgaggtgc ggaagcaggt agccatcgat
     3781 gaggacggcg gtgccgaagc cggtgccgag ggtgacggcg agttcgaagc ctttgccgct
     3841 caccacgccg aggccgagtt ggtcggcgtc attgacgagg cggacgggtc ttttgagggc
     3901 gtcggcgatc tcctgcccca gggggacttc tttccagctg gcggtgaaga ggttgggggc
     3961 ggtgtatacg acgccgttgc ggacgtagcc ggggaagccg acggagacct tctcatagcc
     4021 gggcatgtct ttggcaagtt gttggatgat gtcgatgacg ccggcggcgg tgacatgggc
     4081 gggggtgatg aatttcttat agtctgtttg ttgttcgcct tttgtgttga ggatggtgcc
     4141 tttgatatga gatccgccga tgtcgatagc caggatgttg ccctccatag tggtgttttg
     4201 tttttttaaa ggtacgggtt tgtcgtcgat cccggggccg cggcggattt ttgcgtt
//
LOCUS       DATGFG010000968         6270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1319689, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000968 DATGFG010000000
VERSION     DATGFG010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319689"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2928)
                     /locus_tag="VKQ52_18250"
     CDS             complement(<1..2928)
                     /locus_tag="VKQ52_18250"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ89207.1"
                     /translation="MRSIVYFIFLSLAGVVARAGDDPRVKCLGIEQGLSNNALTCVYQ
                     DARGFMWFGTYDGLNRYDGYGFTVYRTIIGDSTSIPFNNIATICGDGHGNLWVGGQKG
                     LGVFDPVAAKFSVPMFLPFNGGRIVAVRENVHHLHSLGEDLVLAGTHSNGLLVFERGS
                     QVGRQIALVDPASRQAVGHYDVPCISYDSVRGYIWLFIQKRGLYRYDRRARRLLLVND
                     QLRDGLGLQVDTRGRVWLGTDNGLFLLDADGRGYSGNWMPQKCRVVGIHVNRKNELCI
                     GTDGAGVWKLCGDATVATPLVSSAGTPLVNSNSAYAIYEDAEGRKWIGTLRGGINVIE
                     PRESSFKTVVYDPPGERSIVNNFILSFCEDARHNVWIGTDGAGLRYWDRKKNFFTEYV
                     NDPRDEGSISSNFVTNILRDEQDRLWVATWFGGVNRMDKATGKFVHYSCVNTRTNSEE
                     KHVWQLFEDRAHRLWASATNDGCLYLFNRAADRFELFDDAIQSLQSLTEDRAGVMWGG
                     NYTMLIRIDRERRRHMFYPIGYPVRCIHEDRSGQFWVGTQEGGLLLFDRKTGQYKRFT
                     TAEGLPGNTILRILEDAHGNLWLSTYNGISEFQRVEGKFRNFSISDGLQSTQFSFNAG
                     VALSSGEFLFGGIKGFNLFYPDSVLQVRKTPRVYLTGVRVQNEAIEANSRFVTNRETE
                     QIRELTLPFDQAMVSLDFTALEYDGTDKINYAYSLAGWDKGWNYSNGSRTANYSSVRE
                     GAYVFRVRVSHADGTWSREYALLRLVVLPPWHRTWWAYGLYAFCFAGLIWGYVWYTRG
                     RERLRYEVKLAQLETEKEKELVARKVAFFTHITHEFRGPLTLIINPVRELLADRDGKE
                     SLPFVYRNAQRLLGLVDQLLLFQKSESGSDVIKPAPVNIVELAREVWLCFVEGARMKK
                     IGYRFEAPPAAEICLYVDREKVEIALYNLLSNALKYTPEGGEVVFALEDREDGVEIVV
                     RDTG"
     gene            2969..4408
                     /locus_tag="VKQ52_18255"
     CDS             2969..4408
                     /locus_tag="VKQ52_18255"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HLZ89208.1"
                     /translation="MSELQLQRFRKLVWLALIVIAAQSKAQHLIPVAPAWAKNSINTV
                     VFRKNSLTSFNDIQYIAFYDSTSHLVLGRRKLSANGKLTNNKLATNNKLSANSNSNST
                     WQLRQTPYLANTRDAHRSISIIVDGAGYLHCCFDQHDSRLRYARSTAPGSLELGPELP
                     MTNTGETRVTYPEFYRLPTGDLLFLYRDGASGNGNLILNHYDTKTKTWTRLQDKLIDG
                     EGKRNAYWQATLDTKGTLHLSWTWRESPDVASNHDIAYARSRDGGHNWETSDGKSYTL
                     PITAATAEYAAHIPQHSGLINQTSMCADDNGHPYIATYWRPDTSPIPQYQVVYKNNSG
                     WHTIQASNRKTPFLLGGAGTKRIPMSRPQILTAAGKLYLLYRDEEQGDAITLATCAHL
                     PTDPNLPSNSHLPTDPALPATTNSPTGPWTIQSLATGNYGNWEPTFDTQRWAAKHELD
                     LFVQRVGQGDGETLENLPAQPISVLEWRP"
     gene            4460..5746
                     /locus_tag="VKQ52_18260"
     CDS             4460..5746
                     /locus_tag="VKQ52_18260"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLZ89209.1"
                     /translation="MRKLLLGIALLTTAATTHAQLTAPEKKAIDYIDRHMTDAMQLLI
                     NSVNINSGTLNIAGVRKVGELYAAELRKLGFTVEWVSEPDSLHRAGHLVATHTGKKGK
                     KLFLIGHLDTVFEPDMPAGPYTVLNDSTATGQGVNDMKGGDVVMITALQALEAAGQLK
                     DMNIIAYFTGDEERSGSPHSVARKDFIERAKTCDIALAFESAMGLNTVAAARRGGSGW
                     TLDVTAKTGHSATVFTDSAGYGAIYEAARILNTFREQLSTEKYLTFNPGLIVGGSSVT
                     LDPQDARAEALGKTNIISPTAHVTGDLRFLTEAQKEAAREKMRTIVANSLPGTHATIR
                     FSDILPAMEPTPGNLRLVAQLDKVTTDLGFGPTVAGDPGARGAGDISDIAKYLDCLDG
                     LGASGKGAHKAGETINLKEYPLLIHRAAVFLYRLSR"
     gene            complement(5703..>6270)
                     /locus_tag="VKQ52_18265"
     CDS             complement(5703..>6270)
                     /locus_tag="VKQ52_18265"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HLZ89210.1"
                     /translation="NTMVFGDTAVKTWLFNTGAAAGTGKALVVGSNAANGNGAYLTTG
                     GVWTNASDRAKKDHFVVVDRDEILDKIRQLPIARWNYKGLEEQHIGPVAQDFYRLFQV
                     GTDDKTISTIDPSGIALVGVQGLSEKLERQQQEIAQLEGRLAAGQDAILRQQSVIEGL
                     VKQLRVMQQAQAGGSTGKGDKGRRRRGG"
BASE COUNT         1437 a         2403 c         1324 g         1106 t
ORIGIN      
        1 ccccgtatcc cgaacgacta tttccactcc atcctcccga tcctccaacg caaaaaccac
       61 ctccccgccc tccggggtat acttcaacgc attcgaaagc aggttataca acgcgatctc
      121 caccttctcc cggtccacat acaaacagat ctccgccgcc ggcggcgcct cgaagcggta
      181 cccgatcttc ttcatcctgg ccccctccac aaaacaaagc caaacctccc gcgccagctc
      241 cacgatattc acgggcgccg gcttgatcac atccgacccg ctctccgact tctggaacaa
      301 caacaactga tccaccaatc ccagcaaccg ctgcgcgttc cggtacacga atggcaggct
      361 ctcttttcca tcgcggtccg ccaacaactc ccttacaggg ttgatgataa gcgtaagcgg
      421 cccgcgaaac tcatgcgtaa tatgcgtaaa gaacgccacc ttcctcgcca ccagctcctt
      481 ctccttctcc gtctccagct gcgccagctt cacctcgtat cttaaccgtt cccggccccg
      541 cgtataccac acataccccc agatcaatcc cgcaaaacaa aacgcataca acccgtacgc
      601 ccaccaggtc cggtgccagg gcggcaacac cacgagccgc agcaaggcat actccctgct
      661 ccacgtccca tccgcatgac ttaccctaac gcggaaaaca taagccccct cccgaacact
      721 cgaatagttc gccgtccggc ttccattcga atagttccac cccttatccc accccgccag
      781 gctataagca taattgatct tatccgttcc gtcatactcg agggccgtga aatccagcga
      841 caccatcgcc tggtcgaagg gcagggtcag ctcccggatc tgttccgtct cccggttcgt
      901 gacaaaccgc gagtttgctt ctatcgcctc gttctgcacc ctcacccccg tcaggtacac
      961 cctcggggtc ttcctcacct gcaacacgct gtccgggtaa aacaggttga accccttgat
     1021 cccgccaaac agaaactccc ccgacgagag cgcaaccccc gcgttaaaac taaactgcgt
     1081 gctctgcaat ccgtcggaga tcgagaaatt ccggaacttc ccctccaccc gctgaaactc
     1141 cgagatgccg ttatacgtac tcaaccacaa attgccatgc gcatcttcca atatccgcag
     1201 gatcgtattg cccggcaacc cctccgccgt ggtaaatcgt ttgtattgcc ccgtcttccg
     1261 gtcgaacaac aacagccctc cttcctgtgt ccccacccag aactgtccgc tcctgtcctc
     1321 gtgtatacac cgcacaggat acccgatcgg gtaaaacata tgcctcctgc gctcccggtc
     1381 gatccggatc agcatcgtat agttaccgcc ccacatcacc cccgcacggt cctccgtcag
     1441 cgattgcaga ctctggatcg cgtcgtcgaa aagttcgaag cggtccgccg cgcggttgaa
     1501 caggtacaaa cacccgtcgt tggtcgcact cgcccaaagc cggtgtgccc ggtcctcgaa
     1561 caattgccag acatgtttct cctccgaatt cgtccgcgtg ttaacgcagg aataatgcac
     1621 aaacttcccc gtggccttgt ccatgcggtt gaccccgccg aaccaggtcg ccacccaaag
     1681 ccggtcctgt tcatcccgta aaatattcgt aacaaaattg ctgctgatcg atccttcatc
     1741 ccgcggatca ttgacatact cggtaaagaa gttcttcttc cggtcccaat accgcaaccc
     1801 cgccccgtcg gtcccgatcc atacattatg ccgcgcatcc tcgcaaaaag aaaggataaa
     1861 attgttgacg atgcttctct cgcccggcgg atcatacacc actgtcttaa aactgctttc
     1921 ccgcggttcg atcacattga tcccgccgcg cagcgtaccg atccacttgc ggccttccgc
     1981 gtcttcatag atcgcatagg ccgaattgct gttcaccaac ggcgtcccgg cactcgacac
     2041 aagcggcgtc gccaccgtcg cgtcgccaca taatttccac acccccgcgc catccgtccc
     2101 gatgcataat tcgttcttcc ggttgacatg gatccccacc acccggcatt tctgcggcat
     2161 ccagttcccc gaatatccgc gcccatccgc atccagcagg aacaagccgt tatccgtccc
     2221 cagccacacc cgcccccggg tatccacctg caagcccagc ccatcccgca actggtcatt
     2281 caccagcaac aaccgccggg cccgccggtc atagcggtac agccccctct tctggatgaa
     2341 caaccagata tacccgcgaa cactgtcata actgatacag ggaacatcat aatgacccac
     2401 cgcctgacgc gacgccgggt ccaccagcgc gatctgccgc cccacctggc tcccccgctc
     2461 gaacacgaga aggccattgg aatgcgtgcc cgccagcaca aggtcttcgc caagggaatg
     2521 caggtgatga acgttctccc tgacagcgac aatcctaccc ccattaaaag gaaggaacat
     2581 cggcaccgag aacttcgcgg cgacgggatc gaacacccca aggcctttct ggccgccaac
     2641 ccaaaggttg ccgtggccgt cgccgcagat cgtcgcaata ttgttgaaag gaatcgaggt
     2701 cgaatccccg atgatcgtcc ggtaaacggt aaacccgtat ccgtcatagc ggttgagccc
     2761 gtcataggtg ccgaaccaca taaaacccct ggcgtcctgg tagacgcagg tcagcgcgtt
     2821 gtttgaaagg ccctgctcga tgccaagaca tttcacccgc ggatcatccc ccgcccgcgc
     2881 caccactccg gcaagggaca aaaaaataaa gtagacaatt gatctcataa gcagcaatcc
     2941 aatgtaatct acctaaattc ggaaatatat gtcagaatta cagctacaac gttttcgtaa
     3001 actggtttgg ctggccctaa tagttatcgc cgcccaatca aaagcgcaac acctcatacc
     3061 cgtcgccccc gcctgggcaa agaactccat caacaccgtc gtcttccgca aaaactccct
     3121 cacatccttc aacgacatcc aatacatcgc cttctacgac agcaccagcc acctcgtcct
     3181 cggccgccgc aaactcagcg ccaacggaaa actcaccaac aacaaactcg ccaccaacaa
     3241 caaactcagc gccaacagca actccaactc cacctggcaa ctccggcaaa ccccatacct
     3301 cgccaacacc cgcgacgccc accgctccat cagcatcatc gtcgacggcg caggctacct
     3361 tcactgctgc ttcgaccagc acgacagccg cctccgctac gcccgctcca cagccccggg
     3421 ttccctcgag ctcggccccg aactccccat gaccaacacc ggcgagaccc gcgtcaccta
     3481 ccccgaattc tatcgccttc ctaccggcga cctcctcttc ctctatcgcg acggcgcctc
     3541 cggcaacggc aacctcattc ttaatcacta cgacacaaaa acaaaaacct ggacccgcct
     3601 gcaggacaaa ctcatcgacg gcgaaggcaa gcgcaacgcc tactggcagg ccaccctcga
     3661 tacaaagggg accctccacc tctcctggac ctggcgcgaa tcccccgacg tggcaagcaa
     3721 ccacgacatc gcctacgccc gctcccgcga cggcggccac aactgggaga cctccgacgg
     3781 caaatcctat accctcccca tcaccgccgc caccgccgaa tacgccgccc acatcccaca
     3841 acacagcgga ctcatcaacc aaacgtcgat gtgtgccgat gacaacggac atccctatat
     3901 cgccacctac tggcgccctg atacttcccc catccctcaa taccaggtcg tctataaaaa
     3961 caacagcggc tggcatacca tccaggcctc caaccgcaag acccccttcc tcctcggcgg
     4021 cgccggcaca aaacgcatcc cgatgtcccg cccccagatc ctcaccgccg cgggcaaact
     4081 gtatctcctc taccgcgacg aagaacaggg cgacgccatc accctcgcca cttgcgccca
     4141 cctcccaacg gaccccaacc tcccaagcaa ctcccacctc ccaacggacc ccgccctccc
     4201 cgccaccacc aactcaccca ccggcccctg gaccatccaa tccctcgcca ccggcaacta
     4261 cgggaattgg gaacccacct tcgacaccca acgctgggcc gcaaagcacg aactcgatct
     4321 cttcgtccag cgcgtcggcc agggcgacgg cgaaaccctc gaaaacctcc cggctcaacc
     4381 catctccgtc ctcgaatggc gcccctaaaa tccaccccac cgccacgcgg acaaccataa
     4441 aattcctatc ttagctaaca tgagaaaact actgctcggc atcgccctcc tcacgacggc
     4501 cgcaaccaca cacgcccaac tcaccgcccc ggaaaaaaag gccatagatt acatcgatcg
     4561 ccatatgacg gacgccatgc agcttctcat caactccgtc aacatcaaca gcggcacact
     4621 caacatcgcc ggcgtaagaa aggtcggcga gctctacgcc gccgaactcc gcaaactcgg
     4681 cttcaccgtc gaatgggtaa gcgaacccga ctccctccac cgcgccggtc acctcgtcgc
     4741 cacccacacc ggcaagaagg gcaaaaaact cttcctcatc ggccacctcg acaccgtctt
     4801 cgaacccgat atgcccgcgg ggccgtacac ggtcctgaac gactccaccg ccaccggcca
     4861 gggcgtcaac gacatgaaag gcggcgacgt cgtcatgatc accgccctcc aggcactcga
     4921 agccgccggg cagttgaagg acatgaacat catcgcgtat tttacaggag atgaagagcg
     4981 gtcgggctcg ccgcactccg tggcaagaaa ggacttcatc gaaagagcga agacctgcga
     5041 catcgccctc gccttcgaat cggcgatggg cctcaacacc gtcgccgccg cccgtcgcgg
     5101 cggaagcggc tggacactcg acgtcaccgc aaagaccggc cactcggcca ccgtcttcac
     5161 cgactccgcc ggctacggcg ccatatatga agccgcccgc atcctcaata ccttccgcga
     5221 gcagctcagc accgaaaaat atctgacgtt caaccccggc ctcatcgtcg gcggctcctc
     5281 ggtcaccctc gacccgcagg acgcccgcgc cgaagccctg ggcaaaacaa atatcatctc
     5341 ccccaccgcc catgttaccg gcgatcttcg ctttctcacg gaagcacaga aagaagccgc
     5401 ccgcgagaaa atgcgcacca ttgtggccaa ttctcttccc ggcacccacg ccaccatccg
     5461 cttcagcgac atcctgcccg cgatggaacc cacccccggc aacctccgcc tcgtcgcaca
     5521 actcgacaaa gtcaccaccg atctcggctt cggccccacc gtcgcaggcg accccggcgc
     5581 ccgcggcgcg ggcgatatct ccgacatcgc aaaatacctg gattgtctcg acggtctcgg
     5641 cgcctccggc aagggcgccc acaaagcagg tgaaaccatc aacctgaaag aatatcccct
     5701 cctcatccac cgcgccgccg tcttccttta tcgcctttcc cggtagaccc gcccgcctgc
     5761 gcctgctgca ttaccctcaa ttgctttacc aggccttcga tcacggattg ctgccgcagg
     5821 atcgcgtcct gcccggccgc cagccggccc tccagctgcg ctatttcctg ctgctgccgt
     5881 tccagttttt cagatagtcc ctgtacaccc accaacgcaa tgcccgatgg gtcgatggtg
     5941 gagatggtct tatcatccgt ccccacctgg aacagccggt agaaatcctg cgccacggga
     6001 ccgatatgct gctcctccaa ccccttgtaa ttccaccggg cgatcggcaa ttgcctgatc
     6061 ttgtccagta tctcgtcccg atccaccacg acaaaatgat ccttcttcgc ccggtcggaa
     6121 gcgtttgtcc ataccccgcc cgtcgtaaga taggcgccat tgccattcgc agcgttcgag
     6181 cccaccacca gcgccttgcc cgtccctgcc gccgcccccg tattgaacag ccaggtcttc
     6241 accgccgtat cgccaaagac catggtattc
//
LOCUS       DATGFG010000969         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_597561, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000969 DATGFG010000000
VERSION     DATGFG010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597561"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VKQ52_18270"
     CDS             <1..619
                     /locus_tag="VKQ52_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89211.1"
                     /translation="PTMERYDCNMRLGSYTINKGLLENLAQFFTETVAHTISPELAGV
                     KIEEHTVITLLLPGERIRFNNISTFAEYGFNNHIEGVTIELEKLVRTPTYQKAFVFQL
                     SFGKEVADNYLHIALQDSDAGRKLVLICQQCFAKLEPFRNNHSRFYRSEALPTAFFVA
                     GAICGTLAFAVPVPLVGVLLAVIATVGLSLFAYSVVKGYSSFELA"
     gene            complement(676..981)
                     /locus_tag="VKQ52_18275"
     CDS             complement(676..981)
                     /locus_tag="VKQ52_18275"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLZ89212.1"
                     /translation="MAVSYQLHAEAHREFIEAFEWYEFRQNGLGQRFMDQVEKRLQQI
                     NLHPEYYGKHHLNFRQVKVEEFPYMIVYEYFPRKKIIYILAIYHQRRNPKARYRRMR"
     gene            complement(981..1226)
                     /locus_tag="VKQ52_18280"
     CDS             complement(981..1226)
                     /locus_tag="VKQ52_18280"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HLZ89213.1"
                     /translation="MTTAAIRKKLTDYLKVADEQKVKAIYTMVKDEIDTDTNDWSPAF
                     IKELDRRRKEVVNGSAQTYSWEETKKAALGRVKSKKK"
     gene            complement(1358..>2321)
                     /locus_tag="VKQ52_18285"
     CDS             complement(1358..>2321)
                     /locus_tag="VKQ52_18285"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HLZ89214.1"
                     /translation="EQRNATTLWSSDIECTWHSFQDQVPQGINACASGIPYWTSDIGG
                     YHYHWQAPDWSRPEMRELFTRWFQFGTFCPIFRIHGKGERALYSRNWDETTRSVLLTY
                     DNLRYRLLPYIYSMAGKVTQEQYTMMRALPFDFREDTAVYSIKDEYMFGPAFLVNPVS
                     VPGATSRSVYLPNAAWYDFWTGKRLAGGVRMDADAPVATMPLYVRAGSIVPMGPEEEY
                     ATQKPADTLELRVYPGADGQFTLYEDENEGYGYEQGAFATTTIVWKDKTRTLSIGAAK
                     GDFPGRLKKRVFNVVVVGEGKGVGEAPAKGKVVKWVGVPVSAVL"
BASE COUNT          486 a          776 c          542 g          517 t
ORIGIN      
        1 accaaccatg gaaagatacg attgcaacat gcggctgggc agctacacca tcaacaaggg
       61 attgcttgaa aatctcgccc aattcttcac cgaaacagta gcgcacacca tctcccccga
      121 actcgcaggc gtcaaaatag aggagcacac cgtcatcacc ctcctcctgc cgggcgagcg
      181 catccggttc aacaacatca gcaccttcgc cgaatacggc ttcaacaacc acattgaagg
      241 agtcactatc gaactggaaa agcttgtccg cactcccacc tatcaaaagg ccttcgtctt
      301 tcaactctcg ttcggcaagg aggtggccga caactatctc cacatcgcgt tgcaggatag
      361 cgatgccggg cggaagctgg tcctcatctg ccagcagtgt ttcgcaaaac tggaaccctt
      421 taggaataat cacagcagat tctatcgcag cgaggcatta cccaccgcct tcttcgtcgc
      481 cggcgcgatt tgcggcaccc tggccttcgc cgttcccgtg ccgctggtgg gagtgttgct
      541 ggccgtcatc gcgacggtcg ggctatcctt attcgcgtat agcgtggtca agggttattc
      601 gagcttcgaa ctggcctgag tcggccgtgg gcgttccccg ccgccgcgcc ggcttccatc
      661 ctaccgtgtc gcggcctacc gcatgcgccg gtatctcgct tttggattcc gccgttgatg
      721 gtaaatggct aaaatgtaaa ttatcttttt cctggggaaa tattcgtaga cgatcatgta
      781 ggggaactct tctaccttca cctgcctgaa atttaaatgg tgtttgccgt aatattcggg
      841 atggagattg atttgctgca gccgcttctc cacctggtcc ataaagcgct ggcccaatcc
      901 gttctgccga aattcatacc attcgaaagc ctcgataaac tcccgatggg cttccgcgtg
      961 aagttggtac gaaacggcca ttatttcttc tttgatttca ccctgcccaa ggccgccttc
     1021 ttcgtctcct cccacgaata ggtttgagcc gatccattta caacttcctt gcggcgccgg
     1081 tcgagctcct tgataaaagc gggagaccaa tcgttcgtgt ccgtatcgat ctcatctttt
     1141 accatggtat aaatggcttt gaccttttgt tcatcggcaa ccttcaaata gtccgtcaat
     1201 ttttttctta tcgctgccgt cgtcataaaa atagtttact gatttgctga cataccaaat
     1261 atacaactta ttactgcttc ttcccagccg caccgccctc ctttggcaac ctcggtttcg
     1321 cgccagccct accttctcca ccgccgcggc tgcgcaccta caacaccgcg ctcaccggca
     1381 cccccaccca cttcacgacc ttccccttcg ccggcgcctc acccacaccc ttgccctcgc
     1441 caaccaccac cacattgaac acccgcttct tcagccggcc ggggaaatcc cccttcgccg
     1501 cgccgatgga caaggtcctc gtcttatcct tccaaacaat agtcgtcgtc gcaaacgccc
     1561 cctgctcata cccatacccc tcgttctcat cctcgtacag ggtaaactgc ccgtccgcgc
     1621 cgggatagac ccgcagctcg agcgtatccg ccggcttctg cgtggcatac tcctcctcgg
     1681 gccccatcgg gacaatggac cccgcacgca catacaaggg catcgtcgcc accggcgcat
     1741 ccgcatccat cctaaccccg ccggccaacc gcttgcccgt ccagaaatca taccaggccg
     1801 cattcggcag gtagacgctg cggctcgtcg cgccgggcac cgagacgggg ttcaccagga
     1861 acgccggccc aaacatatac tcgtctttta tggaatacac cgccgtgtcc tcgcggaagt
     1921 cgaagggcag cgcccgcatc atcgtatact gctcctgcgt caccttccct gccatggaat
     1981 agatataggg caacaaccgg tagcgcaggt tatcataggt cagcaacacg ctccgcgtcg
     2041 tctcatccca gttgcgcgaa tacagcgccc gctcgccctt accgtggatg cgaaagatcg
     2101 ggcaaaaagt accaaactgg aaccatcgcg taaacagttc ccgcatctcc ggccgcgacc
     2161 agtccggcgc ctgccagtgg taatggtagc cgccgatatc cgaggtccag tacgggatcc
     2221 ccgacgcaca tgcgttgatc ccctgcggca cctggtcctg gaacgaatgc caggtgcact
     2281 cgatatccga ggaccagagc gtcgtcgcgt tgcgctgctc g
//
LOCUS       DATGFG010000970         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_167097, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000970 DATGFG010000000
VERSION     DATGFG010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167097"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VKQ52_18290"
     CDS             complement(<1..538)
                     /locus_tag="VKQ52_18290"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLZ89215.1"
                     /translation="MKGIILAGGSGTRLHPITYAISKQIMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPHDLPLFRKLFGDGSQLGLSFSYAEQPLPNGLAQAFVIGKPFVGTDPV
                     ALVLGDNIFYGAGLGKQLEKNTRPDGGIVYAYQVSDPERYGVVEFDSANKAISIEEKP
                     AKPKSNYAVPGLYFYDNSV"
     gene            complement(621..1658)
                     /locus_tag="VKQ52_18295"
     CDS             complement(621..1658)
                     /locus_tag="VKQ52_18295"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HLZ89216.1"
                     /translation="MPFLVVIIILALVFDYINGFHDAANSIATIVSTKVLTPFQAVLW
                     AAVFNFAAFFIARYILKEFKIGNTIANSVNRDFINLDVIFSGLIAAICWNLLTWWFGI
                     PSSSSHTLLGGFMGAALAHAGALSRNGVDVINYAKVVPTFLFIFVAPFIGMVVAFIIT
                     LIILNVCRHANPYRADNWFRRLQLLSSGMLSLGHGSNDAQKVMGIIGAAMIWYEKSRG
                     HEMSFKQFIDSNMWVPLASFTAIALGTLSGGWKIVKTMGTRITKVTPLEGVSAETAGA
                     ITLFLSDKLGIPVSTTHTITGSIIGVGATKRLSAVRWGVTINLLWAWVLTIPVSALLA
                     ALVYFIVSFFR"
     gene            complement(1664..2311)
                     /locus_tag="VKQ52_18300"
     CDS             complement(1664..2311)
                     /locus_tag="VKQ52_18300"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HLZ89217.1"
                     /translation="MAFQSFLQLFTPKDRVFYSLFEEVADSVAKMGKLMKEAVAEPDA
                     DKRAAISSQVEDQEHVNDDLTHRIFTELGRNFITPFDREDIHYLASSLDDICDYIYAA
                     VKKINFYKVNPNDTGIQKLAELIDQGTVQIQIAVKELRNMRDMRKITDALVKVNSIEN
                     QADDIFDLSIERLFESEPDAKEVIKKREIYQVMEIVTDKCEDAANVIESIIIKYA"
     gene            complement(2337..>3387)
                     /locus_tag="VKQ52_18305"
     CDS             complement(2337..>3387)
                     /locus_tag="VKQ52_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89218.1"
                     /translation="PVARTKHLFIVTIDGFRWQEVFTGADPLLVANPDYVLDTALTRG
                     MYWDSTSELRRQKLLPFFWGTLAAKGRLYGNRLYGNKVNVKNFYKISYPGYNEIFTGH
                     TDAISSPNIPINNKHVNVLEYLNASPDYQGKVAVFTSWNVFPFILNEDRSGLPVNSGY
                     HRLGEESDEVAGLIDSVQATMHQSKTRHDYLTFCSAREYMRLHHPSVLFLGFGETDEC
                     AHAGRYDLYLQQAADIDRMIADLWYMIQTDPYYKDSTTLLITTDHGRGWKPNKWTTHG
                     FWAEGSGDIWMAVLGPDIQAEGEIRKRGQVYQKQMAATMATLLGNPVAPGHPPGRAIL
                     FPAVRPVADLAKLVE"
BASE COUNT          732 a          893 c          993 g          769 t
ORIGIN      
        1 cgacggaatt atcatagaaa tagagacccg gaacagcgta gttgctcttg ggctttgccg
       61 gtttctcttc tatggaaatc gccttgttgg cggaatcgaa ctccacgacc ccgtaccgct
      121 ccgggtcgct gacctggtag gcatagacga tcccaccgtc cgggcgtgta ttcttctcca
      181 gttgcttgcc cagtcccgcg ccataaaaaa tattatcgcc cagcaccagc gccaccggat
      241 cggtaccgac aaagggcttg ccgatgacaa aggcctgtgc gagtccgttt ggcaggggtt
      301 gttcggcata cgagaaggac aggccgagct gcgagccgtc gccaaagagt ttcctgaata
      361 aaggcagatc gtgtggcgtg gaaatgatca ggatatcctt gatccccgcc agcatcaggg
      421 tagacagggg atagtagatc atcggtttgt catagaccgg catgatctgt ttgctgatgg
      481 cataggttat cggatgtaga cgtgtaccag accctcctgc gagaataatt cctttcatag
      541 ttctaaaacg ggttttgaaa atcttcgaaa gataataaag ttattgcaac tggcaatgca
      601 tacagcggat cccgggggga ttaccggaag aaactaacga taaaataaac cagcgccgca
      661 agaagggcgc ttaccgggat cgtcagcacc caggcccaca gcaggttgat ggtcacaccc
      721 catctcaccg cggagaggcg tttggtggcg cccaccccga tgatcgaccc ggtgatggta
      781 tgggtggtgc tcaccgggat ccccagtttg tcggagagaa aaagggtgat ggcgccggcg
      841 gtctctgcgc tgaccccttc caggggcgtc actttcgtga tcctcgtgcc catggtcttt
      901 acgatcttcc agcccccgct cagggttccc agcgcgatgg cggtaaagct ggccagcggc
      961 acccacatat tggaatcgat aaactgtttg aatgacattt catgtccccg ggatttttca
     1021 taccagatca tggccgcgcc gatgatgccc atcactttct gtgcatcgtt gcttccgtgc
     1081 ccgaggctca gcatgccgga ggaaagcagt tgcagccgcc ggaaccagtt atccgcccga
     1141 taggggttgg cgtggcggca tacgttcagg atgatcaggg taatgataaa ggcaaccacc
     1201 atcccgataa agggcgccac gaagatgaag aggaaggtgg gcaccacctt ggcatagttg
     1261 atgacatcga cgccgttgcg gctcagcgcc ccggcgtgcg ccagcgccgc ccccatgaag
     1321 ccgcccagca gcgtatggga agaactggac gggatgccga accaccaggt gagcaggttc
     1381 cagcaaatgg ccgcgatcag tcccgagaaa atgacatcca ggttgatgaa gtccctgttg
     1441 acactgttgg cgatggtatt gccgatcttg aattccttca ggatataccg ggcgataaaa
     1501 aaagcggcga aattgaagac ggcggcccag agcaccgcct ggaagggcgt cagcaccttg
     1561 gtggagacga tggtggcgat ggagttggcc gcgtcgtgaa aaccgtttat gtaatcgaat
     1621 acgagggcca gtatgataat gactaccagg aagggcatgg ggctcaggcg tattttatga
     1681 tgatggattc gatgacattc gcggcgtcct cgcatttgtc agtgacgatc tccatcacct
     1741 ggtagatctc gcgcttcttg atcacctcct tggcgtccgg ctcggactcg aaaaggcgtt
     1801 cgatgcttag atcaaagata tcatccgcct gattctcaat actgtttact ttaacaaggg
     1861 catcggttat cttccgcatg tcccgcatat tccgcagttc cttcactgcg atctggatct
     1921 ggaccgtccc ctggtcgatc agttcggcca gtttctggat accggtgtcg tttgggttta
     1981 ccttgtagaa attgatcttt ttcacggccg cgtagatata gtcgcagata tcgtccaggc
     2041 tgctggccag gtagtggatg tcctcgcggt cgaagggcgt gataaagttg cggcccagtt
     2101 cggtgaagat gcgatgggtg aggtcatcat tgacatgttc ctggtcttcc acctgggagg
     2161 agatggccgc ccgtttgtcg gcatccggct ccgccacggc ctctttcatc agtttaccca
     2221 tttttgcgac gctgtcggcc acttcttcga acagggaata aaatacgcgg tcttttggag
     2281 taaaaagctg gagaaaggac tggaaagcca tgcggaaaat ttgcgcaaag gtagtgttat
     2341 tcgaccaatt tcgccaggtc tgccacgggc cggacggccg gaaagagaat agcccggccg
     2401 ggcggatgac cgggagcgac cgggttaccc agcagggtgg ccatggtggc cgccatttgt
     2461 ttctgataga cctggcctct tttacggatc tcgccttctg cctggatatc aggccctaat
     2521 acggccatcc agatatcccc cgagccttcg gcccagaaac cgtgggtggt ccatttgttg
     2581 ggtttccagc cccggccatg atcggtggtg atgagcaggg ttgtactgtc tttataataa
     2641 ggatcggtct ggatcatata ccagagatcg gcgatcatcc ggtcgatgtc ggccgcctgt
     2701 tggaggtaaa gatcatagcg gccggcatgt gcgcattcgt ccgtctcgcc aaacccaaga
     2761 aagaggacgg aaggatggtg aagccgcatg tattcgcggg cgctgcagaa ggtgaggtaa
     2821 tcatgccggg tcttgctctg gtgcatcgtt gcctgcacgc tgtcgatcag ccccgccacc
     2881 tcatcgcttt cctcccccag ccggtggtac ccgctgttga cgggcaggcc gcttcggtct
     2941 tcgttgagga taaaggggaa cacgttccag gaggtgaaaa cggccacttt cccctgatag
     3001 tccggactgg cgttgagata ttccaacaca ttgacgtgct tgttattgat ggggatattg
     3061 ggactgctga tcgcgtcggt atgacccgta aagatctcgt tgtaacccgg gtaggagatc
     3121 ttgtagaaat tcttcacgtt caccttgttc ccgtagagcc ggttgccata cagccggccc
     3181 tttgcggcga gcgtgcccca gaagaagggc agcagcttct gccggcgcag ttcggaggtg
     3241 ctgtcccagt acatcccccg cgtgagcgcc gtatccagga catagtcggg gttggctacc
     3301 agcagcgggt ctgcgccggt aaagacttcc tgccagcgga atccgtcgat ggtgacgata
     3361 aagaggtgtt ttgtccgggc aacaggc
//
LOCUS       DATGFG010000971         5013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1433635, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000971 DATGFG010000000
VERSION     DATGFG010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433635"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VKQ52_18310"
     CDS             <1..1507
                     /locus_tag="VKQ52_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89219.1"
                     /translation="IRIFGEQQTILRTSLYPASGGSNAMFFFLGAYVHCIIDNIFFQN
                     THPLPSGNPSPIGQTNALYLFGTGSVANKGFTVRGCHFEGFSTAITLNGTEDTRIEQC
                     SFSAPYGRDNAQGTNQQPAVYIWMVSNSNGEVINPGIIDCYANGYSGTSPIATGTASK
                     MPMDGFIYGTPEGGLFSGNVLKNMGQELIFVSPYTDSPSTRPVMISNNIVDCAIPAGS
                     VSISNPSVPATANYGIRSEAKNSVITGNTITNVTQGILQYAYTLYNYQFKRWQIQDNV
                     IQFTTNSAMAPQYGIYVQGYNSSTTTRVQYPNIVNNTLIADSLTLNGSDLNVLNVNDA
                     DSALVTGNTLILGKVTRAGHVINFSNMTSCYNASYKDNVIRGVVDATYVIGSSSPAST
                     YTDWDNFSSVTVRQVTANTTLTAADATLIGNAASGSFVITLPSPSDLAVTARGQGKKF
                     VFRNITSGSSNTMTVSTPSGLIIGTSSGSSLVIGSGTTVTLETDGTNWYVL"
     gene            1692..2225
                     /locus_tag="VKQ52_18315"
     CDS             1692..2225
                     /locus_tag="VKQ52_18315"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLZ89220.1"
                     /translation="MATATRTSKNTQSKLREFFIEQLQDIYWAEQKLVKTLPKLANAA
                     HSADLREAFNNHLEETRNHVTRIEQAFRMINEEPDSQECQAIKGIIKEGEDIIDETED
                     ETAQRDVGLIFAGQKAEHYEIATYGGLVQLAKTMGQSDVASLLQQTLMEEKMADQKLT
                     EIAETSSNEAAAREKAD"
     gene            complement(2312..2767)
                     /locus_tag="VKQ52_18320"
     CDS             complement(2312..2767)
                     /locus_tag="VKQ52_18320"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HLZ89221.1"
                     /translation="MIRQTRTLMVSSPEFTQGGIIPVKYTADGEGINPPLEFGDIPED
                     TKSLALIMEDTDAPKGAFTHWILWDIISQDSIGENSNPGVSGVNSGGKMGYLPPRPPS
                     GHHRYFFHIYALDSTLNLRPGANRDALESAMNGHILVRGTLMGRYGTSS"
     gene            complement(2840..3550)
                     /locus_tag="VKQ52_18325"
     CDS             complement(2840..3550)
                     /locus_tag="VKQ52_18325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ89222.1"
                     /translation="MIRCLAIDDETPALDILEDNIRQVPFLQMIGKCKNAYAAMERMQ
                     LQPVDLLFLDIEMPGISGLSFLKSLSNKPMVIFITAYRNYAVEGFDLDVLDYLVKPVS
                     FERFLKAVNKALEYQGFKQKEESPNNNYPDYLFIHTEYQLTKVFIHEITYIEGLRNYI
                     RIHLTGSVKPLLSKLSLKAIEEKLPPGKSARVHKSFIIMLDKISSVRNDIIRIGNRDI
                     PLSRSCREEFFRKINSGT"
     gene            complement(3547..4593)
                     /locus_tag="VKQ52_18330"
     CDS             complement(3547..4593)
                     /locus_tag="VKQ52_18330"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ89223.1"
                     /translation="MPRLKRKYAVFYHIGSWSVLFLCIMLWRSWNHGPREPHLPGAAI
                     VMIGLPYIALYYGHAYWLMPAYLFRQKRTAYILLVLASLLTVVLIAGLIFYEYGLPPA
                     GVTYFQSVSKRFFPGLLILLVSASVGTFRENARLQKIRKDNETEHLRTELSFLRSQVN
                     PHFMLNVLNSMVLLARRKSDLLEPVLLELARLMNYMLYDAGNQKISLEEEISYLRAYI
                     DLQMLRFRDDVKVHFNTPEPIPAKCIEPMLFIPLVENAFKHGIGLVREPVINIDIRKD
                     GENRIKMTVENKYNPLVRLQETRAQGIGLSNLRKRLDLVYPGEYGFEAVDNHEFDTLA
                     KENRFVTTLNIPLQ"
     gene            complement(4607..>5013)
                     /locus_tag="VKQ52_18335"
     CDS             complement(4607..>5013)
                     /locus_tag="VKQ52_18335"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HLZ89224.1"
                     /translation="FTTSSRFRKNQITPIANFQDPPGIPNYYRFEEHINGALFTKDLF
                     VFDDRLSDGRYIQYNLRTDSSYLTQGDLLQVDMYCVDKNDYTYFFQLNRSTSTGTFNT
                     DASPANPSTNISNGAYGYFSAHTVRSRTVAVY"
BASE COUNT         1122 a         1183 c         1549 g         1159 t
ORIGIN      
        1 catccggatc tttggcgagc agcagaccat tctgcgaacc tcgctatacc cggcgtcggg
       61 tggaagcaat gccatgttct tttttcttgg cgcttatgtt cactgtatca tcgacaatat
      121 ctttttccag aacacgcatc ccctaccctc cggcaacccc agccccatcg ggcagacgaa
      181 cgcgctttat ctttttggca ccgggtcagt agccaacaaa gggtttacgg tcaggggttg
      241 tcatttcgaa gggttctcca cggccataac gcttaatggc accgaggata cacggatcga
      301 acagtgtagt ttcagcgccc cctatggcag ggacaatgca caagggacca atcagcagcc
      361 ggcggtgtat atctggatgg tgagcaattc caacggggag gtgatcaacc ccggcatcat
      421 cgattgttat gccaacggat attccggcac atcgccgatc gcgaccggca cggccagcaa
      481 gatgccgatg gacggtttta tctatggcac gccggaaggg gggctttttt cggggaatgt
      541 gctcaagaat atggggcagg agctgatctt tgtcagcccc tatacggatt caccgtcgac
      601 gcgaccggtg atgatctcca acaatatcgt ggactgcgcc attcccgcgg gttcggtctc
      661 gataagcaat ccgtcggttc ccgccacggc caattatggc atccggagcg aagcaaagaa
      721 ctcggtgatc acgggcaata cgatcacgaa tgtgacgcag ggcatattgc aatatgccta
      781 taccctttat aattaccagt tcaagcggtg gcagatacag gacaatgtga tccagttcac
      841 taccaattcc gcgatggcgc cgcaatacgg catttatgtc caggggtata acagttccac
      901 caccacgagg gtccagtatc cgaatatcgt caacaatacg cttatcgccg attcgctgac
      961 gcttaacggg agtgatctga atgtactgaa tgtgaatgac gccgacagcg ccctggtgac
     1021 gggcaatacg ctgatcctcg ggaaggtcac gagggcgggg catgtcatca atttcagcaa
     1081 tatgaccagt tgctacaatg cgagttataa ggacaatgtc atcaggggtg tcgtcgatgc
     1141 gacgtatgtt atcgggtctt cgtctccggc cagcacctat acggactggg acaatttttc
     1201 gagtgtgacg gtgaggcagg tgacggcgaa tacgacgctg acggccgcag atgccaccct
     1261 catcgggaat gcggcatccg gcagcttcgt gatcaccctg ccatcgccat ccgatctggc
     1321 cgtgactgcc agggggcagg gcaagaagtt cgttttccgg aatatcactt ccggatcttc
     1381 caatacgatg acagtatcga cgccaagcgg gctgatcatc gggacgagtt cggggtccag
     1441 tcttgttatc ggctcgggca cgacggtaac gctggaaacg gatgggacga attggtatgt
     1501 gttgtaggct atgcgaggga ggatgcgctc atctcttcgc tgttttgggg cggtcctaac
     1561 ggggattttt ttgcagacag tagaaccgat tccacgggtc aatgcggggg atggaaggcg
     1621 agatcccgcg ggggccaggc gctatgacgg ggtggcgtga tttctgcggc accacccaaa
     1681 acgatataac tatggcaaca gcaacacgta cttcaaaaaa cacccaatcc aaattgcggg
     1741 agttctttat cgagcagctt caggacatct actgggccga gcagaagctg gtaaagacct
     1801 tgccaaaatt ggccaatgca gcgcattccg ccgatctgag ggaggccttt aacaaccatc
     1861 tggaagagac ccggaatcat gtcacgcgga tcgagcaggc tttccggatg atcaacgagg
     1921 agcccgattc acaggaatgc caggccatca aggggatcat caaggaagga gaagacatca
     1981 tcgatgaaac ggaggatgaa acggcacaac gggatgtggg tttgatcttc gccggtcaaa
     2041 aggcagagca ttatgagatc gccacctatg gcggacttgt gcaattagcg aagacgatgg
     2101 gtcagtcgga cgtcgccagc ctgttacaac agacgctgat ggaggagaag atggccgacc
     2161 agaagctgac cgagattgcg gagacgagca gtaatgaagc agcagcaagg gaaaaagccg
     2221 attagcaccg gtggtcgagg ggtcgggagg tggtccaggg gtcaggaggt ggtcgatggg
     2281 tcggcgatca ggagggccgg tcatccggcg atcaggacga agtgccgtat cggcccatca
     2341 gggtgccgcg gacgaggata tggccgttca tggctgactc gagggcatcc cggtttgcac
     2401 ccgggcgtag gttgagcgtg ctgtcgagcg cgtatatgtg gaagaaataa cggtgatggc
     2461 ccgatggcgg ccttggtggc agatagccca ttttgccgcc tgagttgacg ccgcttacgc
     2521 ccgggttgct attctcgcca atgctatcct gagagatgat atcccatagg atccaatggg
     2581 tgaaggcgcc ttttggcgcg tcggtgtcct ccatgatgag ggcgaggctt tttgtgtcct
     2641 ccgggatatc gccgaattcc agcggagggt ttatcccttc accgtcggca gtgtatttta
     2701 ccgggataat gccgccctgg gtgaattcgg ggctggaaac catcagtgtt cttgtttggc
     2761 gtatcataac ttttttggat taggaacaga aaatgttgtg ccggagtcgg gacgttgtgc
     2821 cgatatccgc cggttgagcc tatgttccgg aatttatttt cctgaagaat tcctcccggc
     2881 aggagcggct cagcgggatg tcccggttgc cgatacggat gatatcgttg cgaacggagc
     2941 tgatcttatc cagcataatg ataaaggatt tgtgtacgcg ggcagatttg cccggcggga
     3001 gcttttcttc gatggctttg aggcttagct tggaaaggag gggtttgacg gagccggtga
     3061 ggtggatgcg gatataattg cgcagcccct cgatataggt gatctcgtgg ataaagacct
     3121 ttgtgagttg gtattccgtg tgaatgaaga ggtagtcggg ataattgttg ttcggactct
     3181 cttccttttg tttgaagccc tggtattcga gggccttgtt cacggctttc aggaagcgct
     3241 cgaaggagac cggttttacg aggtagtcca ggacgtcgag gtcgaagcct tccaccgcgt
     3301 agtttcgata ggcggtgatg aaaatgacca tgggtttgtt ggaaaggctt ttcaggaagc
     3361 tcagcccgct gatgccgggc atttcgatat cgaggaagag gaggtcgacc ggttgcagtt
     3421 gcatcctttc catcgccgcg taggcatttt tgcatttccc gatcatctga agaaagggca
     3481 cctgccggat attgtcttcg aggatatcca gcgcgggggt ttcgtcgtct atggcgaggc
     3541 atctgatcat tggagcggga tatttagcgt ggtgacaaag cggttctctt ttgccagcgt
     3601 gtcgaattca tggttatcga cggcttcgaa gccatattcg cccgggtaga ccaggtccag
     3661 ccgtttgcgg aggttgctga gcccgatgcc ctgagcgcgg gtttcctgga gccggacaag
     3721 gggattgtat ttattctcca cggtcatttt tatcctgttc tcgccgtcct tgcggatgtc
     3781 gatgttgata acgggttccc tgaccaggcc gataccgtgt ttaaaggcat tttccacgag
     3841 gggaatgaag agcatgggtt cgatgcattt cgcggggatg ggttccgggg tgttgaaatg
     3901 aacctttaca tcgtccctga agcggagcat ctggagatcg atataggcgc gcaggtagct
     3961 gatctcctct tcgaggctga tcttttggtt gccggcgtcg tataacatat aattcatcag
     4021 gcgggccagt tcgagcagga cgggttccag cagatcggat ttacgccggg cgagcaagac
     4081 catgctgttc aggacattca gcatgaaatg ggggttcacc tgggagcgga ggaaggacag
     4141 ttcggtgcga aggtgttcgg tttcattgtc cttccggatc ttttgcagcc gggcattttc
     4201 gcggaaggtg ccaacgctgg cgcttacgag gaggatgaga agacccggga agaacctttt
     4261 gctcaccgat tgaaaatagg taacccctgc gggcggcagt ccgtattcat agaaaatgag
     4321 gccggcgatc agcacgacgg tgagcaggga tgccaggacc agcaggatgt aagctgtccg
     4381 tttttgacgg aacaggtagg cgggcatcag ccaataggca tgcccatagt agagggcgat
     4441 gtatggaagg ccgatcatga ctatggctgc gccgggcaga tggggctcgc gagggccgtg
     4501 gttccaggac cgccagagca tgatgcatag gaacaatacc gaccagctgc cgatatgata
     4561 gaaaaccgcg tatttccgct ttagtctggg cataggttaa aattagctaa taaaccgcaa
     4621 ccgttcttga ccggacggta tgggcgctga aatagccgta tgctccgttg ctgatattgg
     4681 tggaggggtt ggccggggat gcgtccgtgt tgaaggtacc ggtggaggtg cttcggttca
     4741 attggaagaa ataggtgtag tcattcttgt cgacacagta catgtccacc tgcaggagat
     4801 cgccctgggt cagataggag ctgtccgtgc gcaggttata ctgaatatac cggccgtcgg
     4861 agaggcggtc gtcgaagaca aagaggtcct ttgtaaagag ggcgccattg atatgctcct
     4921 cgaagcgata gtaattaggg atgccgggcg ggtcctggaa attcgctatc ggggtgatct
     4981 ggtttttcct gaaccggctg gatgtagtga agg
//
LOCUS       DATGFG010000972         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_396066, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000972 DATGFG010000000
VERSION     DATGFG010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396066"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VKQ52_18340"
     CDS             <1..476
                     /locus_tag="VKQ52_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89225.1"
                     /translation="LTYTTDIAGSGSALFGSIDNYNKTLYLLGEEADPTFTTGGGRYS
                     PPKTYTARTLDVSYSQRELALLPRVSIANNPFRGYGRWELSIGYNFPLVDRGGIFLKQ
                     VGDGSSKGISGAVNINNSDISASYDGKPIRSAPYRFGGLYAGVLLSLGKYHEESR"
     gene            complement(458..1051)
                     /locus_tag="VKQ52_18345"
     CDS             complement(458..1051)
                     /locus_tag="VKQ52_18345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ89226.1"
                     /translation="MPVSPSNQGSDATPPTPGSPASGIIPAGPADIPALVSLVNSAYR
                     GEEGRQGWTHEAHLISGSRTNAAGIEELLNTPGGVILKYPDATGDLLGCVYLQKEGDK
                     LYLGLLSVQPAQQNTGIGKQLLAAADDYARRQHCDRIHITVISARPELLAWYERHGYR
                     RTGEIEPFHAGEKFGIQKQSLELVVLEKSLSFHRDSS"
     gene            complement(1163..2278)
                     /locus_tag="VKQ52_18350"
     CDS             complement(1163..2278)
                     /locus_tag="VKQ52_18350"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLZ89227.1"
                     /translation="MPQPFATPEQNDQSKRNLMDHVLETPSYGWRDQENNLIIPTSRQ
                     IVREFLFRLNIFRSKKNWLAFFGWARVFLLLPCFVWFFVHYFNFYLLGIAFLYGMVIM
                     GTHGTIWYHRYCTHGAYTFKNNFWRFITRNLTINMVPEEIYVISHHVHHAKSDEPGDP
                     YNASAGFLYCFLADVNHQPISRHLSEADYARVVKLMDHTGIHPNTYRQYQKWGSIANP
                     VRTIAGWLLNWAFWGVVFYWLGGLPLLFTVFGAAGFWAIGVRTFNYEGHGKGKERHRD
                     GTDFNRKDLSINQIWPGYVAGEWHNNHHLFPCSARSGFLNYQVDLAWCYIKLLSRIGA
                     VSAYHDSKQAFLSQYHQPYLESKKAGSLAVAIPESKR"
     gene            2493..3137
                     /locus_tag="VKQ52_18355"
     CDS             2493..3137
                     /locus_tag="VKQ52_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89228.1"
                     /translation="MSHPAFLTFQRFNDPEFASAIAAELKKHDIESRVIKGEAPFDRL
                     LIQNTAGYFVELQLAPADFTRANGVMEEFYAAQLKDVDPDYYLYSFTDAELVEIVAKP
                     DEWGHFDYVFAKKLLADRGNPITEETAELLKEKRLEELEQPEIPPDGVGPMPVPNING
                     VISSHIIATHKKTLPDGRQVYSYAPAQRKRARIFFYIALFILLVLLIEYLRQLP"
     gene            complement(3207..>3412)
                     /locus_tag="VKQ52_18360"
     CDS             complement(3207..>3412)
                     /locus_tag="VKQ52_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89229.1"
                     /translation="WVVLYKEQGYSGRSKDRATYLLKLLSWMIHDGQKFSNDLNYAPL
                     STAAVKVGDNLLKSVTYNGKPVL"
BASE COUNT          754 a          839 c         1043 g          776 t
ORIGIN      
        1 gcctgaccta caccaccgat atcgccggct cgggcagcgc cttgttcggg agtatcgaca
       61 attataacaa aacgctctac ctgcttggcg aggaggcgga ccctaccttt accaccggtg
      121 gcggcaggta cagccctccg aaaacctata ctgcccgaac gctggatgtc tcctattccc
      181 aacgggaact ggccctgttg ccgagggtaa gcatcgcgaa caatcccttt cggggttatg
      241 gccgctggga actgtccatc gggtataatt tccctctcgt tgaccggggc gggatctttc
      301 taaagcaggt gggtgacggt tcttcgaaag ggatctcggg cgccgtgaat atcaataaca
      361 gtgacatttc ggccagttat gacggcaagc cgatacgttc ggcgccctat cgattcggcg
      421 ggttgtatgc gggggtgttg ttgagtttgg ggaagtatca cgaggagtcc cgatgaaaag
      481 agagggattt ttcaaggacc acgagttcca gcgattgttt ttgaataccg aatttttcgc
      541 cggcgtggaa gggctcgatc tcgccggtgc ggcggtagcc gtggcgttcg taccaggcga
      601 ggagttcggg gcgggcggag atgacggtga tgtggatgcg atcgcagtgc tgccggcggg
      661 cgtagtcgtc ggccgcggcc aggagttgtt tgccgatgcc ggtgttttgt tgtgccggtt
      721 gcacgctgag aaggccgaga taaagcttgt cgccttcctt ttgcaggtaa acgcagccga
      781 ggagatcgcc ggttgcatcg gggtatttga ggatgacccc gccgggtgtg ttcaggagtt
      841 cttcgatgcc ggcggcgttg gtgcgggagc cggagatgag atgggcttcg tgggtccagc
      901 cctgccggcc ttcctcgccg cgataggcgc tgttgacgag ggagacaagg gccggaatgt
      961 cggcggggcc cgcggggatg atgccagagg cgggggagcc cggagtggga ggtgtagcgt
     1021 cggaaccttg attggagggg ctgacgggca tgcgtgctgt tttggcaaat atagaatgtc
     1081 gccgcttatt ttactgcttt atatacgaac caatgctcat cgatctgccg gtgcaaccag
     1141 agcccataat cgagatcgcc ttctaccgct tggactctgg aatagcaacc gccagggacc
     1201 cggccttttt gctctcgaga tagggctggt ggtattgtga caggaaggcc tgtttcgaat
     1261 catgataggc gctaacggcg ccgatccggc tgaggagttt tatataacac caggcgaggt
     1321 ccacctggta gttgagaaat ccgctgcggg cgctgcaggg gaacagatga tggttgttat
     1381 gccattcccc ggccacataa cccggccaga tctggttgat ggaaaggtcc ttccggttaa
     1441 aatcggtgcc gtcccgatgc cgttccttgc ccttgccatg cccctcataa ttaaaggtcc
     1501 gcacgccgat ggcccagaag ccggcggcgc cgaataccgt aaagagcagg ggcaggccgc
     1561 ccagccaata gaacaccacg ccccagaaag cccaattcaa cagccagccg gcgatcgtac
     1621 ggacggggtt ggcgatcgac ccccattttt ggtattgccg gtaggtattg ggatgaatac
     1681 cggtgtggtc catcaatttt accacccggg catagtcggc ttcggagaga tgccgggaaa
     1741 taggctgatg gttcacatcc gcaaggaaac aatataaaaa tccggcggaa gcattatagg
     1801 gatcgccggg ttcgtccgat ttggcatggt gcacatggtg cgagatgaca tagatctctt
     1861 ccggaaccat attgatggtg aggttgcggg tgatgaagcg ccagaaatta tttttgaacg
     1921 tatatgcccc atgggtgcaa tagcgatgat accagatggt gccatgtgtg cccatgatca
     1981 ccatcccgta taagaaggcg atgcccagca gatagaagtt gaaataatgc acgaagaacc
     2041 atacaaaaca ggggagcagc aggaataccc gcgcccagcc aaagaaggcg agccagttct
     2101 ttttcgaccg gaagatgttc aggcgaaaca ggaattccct gactatttga cgggaagtcg
     2161 ggatgatgag gttgttctcc tgatctctcc atccatacga aggtgtttca aggacatggt
     2221 ccatcaggtt tcgctttgac tggtcgttct gttcgggggt ggcaaagggc tgaggcatgc
     2281 aattcagggt tattccgcaa tataacggga ttgctgcgat taacgcgcgt agggcgatcg
     2341 aatattttcg cacagtctga atactttctg tatacaattg atcggaggcg gggtgatggg
     2401 gtggatcgga ggtctgctgt tcgggtgttg gatcggggat tggaaaacaa gcggagtttt
     2461 cttattttac cgtacccaaa tccatatcgg ctatgtccca tcctgccttt cttacctttc
     2521 agcgatttaa cgatcccgag tttgcgtcgg ccattgcggc ggaactaaaa aagcatgaca
     2581 tcgaaagccg ggtcatcaag ggagaggcgc cgtttgaccg cctcctgatc cagaataccg
     2641 ccggatattt tgtcgagcta cagttggccc cggccgattt cacccgggcg aacggcgtta
     2701 tggaggagtt ttacgccgcg cagttgaagg atgtcgaccc tgactactat ctgtattcct
     2761 ttaccgacgc ggaattggtg gaaattgtcg caaaaccgga tgagtggggg catttcgact
     2821 atgtattcgc caaaaagttg ttggcggata gaggaaaccc gatcaccgag gaaacggcgg
     2881 agctgctaaa ggaaaagcgg ctggaagagc tggaacaacc ggagatcccg cccgatgggg
     2941 tcgggcccat gccggtgccg aatatcaacg gggtgatctc gagtcatatc atcgccaccc
     3001 ataaaaaaac gcttcccgat ggaagacagg tgtacagcta tgcgccggcc caacgtaaaa
     3061 gggcgaggat cttcttttat atcgccctgt tcatcctgct ggtgctgctg atagaatacc
     3121 tacggcaact tccctaggcc cgatggactc aaatgaataa ggggagatgt cgccatctcc
     3181 cctcgtcgta ttgtataccc cgccttttag aggacgggtt tcccgttgta ggttacagac
     3241 ttgagcaaat tatcaccaac ctttactgcg gcggtggaaa gcggcgcgta gttgagatcg
     3301 ttgctgaact tctggccgtc gtggatcatc caggacagga gtttgagcag gtaggtggcg
     3361 cgatcctttg aacggccgct gtagccctgc tccttataga gtaccaccca gg
//
LOCUS       DATGFG010000973         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_512127, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000973 DATGFG010000000
VERSION     DATGFG010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512127"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VKQ52_18365"
     CDS             complement(<1..915)
                     /locus_tag="VKQ52_18365"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLZ89230.1"
                     /translation="MNSRKRATIYDIAKELAISPSYVSKALNNHPSVGEDIRRRVKDK
                     AEQLNYKHNSHAANLRRGQSRTIGVVVPHINQSFFSDAISGIEEICFAHNHSLVICQS
                     HESFEKEGKAIMTLIHQNVDCILISNAAETTSADHLRQATKNKIPVIQFDRCLDEFDS
                     FKVVNDNRTVSYEAVRSLINEGYRNIAFLGGPVRVKAFQDRKDGFIAAIAEANLPIPY
                     NFIVDNVLTRERGQQAALPLLRLPNRPDAIFTVSDHQSLGVMDAAASVGITIPSGLGV
                     FGFADETFAQLVTPSLSTIDQHSVELGRK"
     gene            977..2035
                     /locus_tag="VKQ52_18370"
     CDS             977..2035
                     /locus_tag="VKQ52_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLZ89231.1"
                     /translation="MEIKLPPVIFGTSGLGNLFVALDEESKMKVVEACILLSGGQVVF
                     DSAGKYGAGLALEVLGRVLRRLNIAPGDVVISNKLGWVRSPLQGEEPTFEPGVWKDLR
                     HDAVQKIGYDGIIECFDQGNELLGGYTPRMVSVHDPDEYLAGAGGRRGLQDQRYEDIL
                     DGYEALFDLKRLGRVSAVGVGAKDWRVIKRIVRDVPLDWVMFANSMTLKSHPWELAYL
                     IKELAERGVTVINSAVFHSGFLVGSDFFDYRRISRGDPVTDPLFRWREELFAVCEEFN
                     VTPAAACVQFALGVPGVSSIALNSSNPDRVKENLELAVRRLPDEFWEVLRERELISFD
                     PLAAVRPGDLINKYTIFR"
     gene            2032..>3538
                     /locus_tag="VKQ52_18375"
     CDS             2032..>3538
                     /locus_tag="VKQ52_18375"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HLZ89232.1"
                     /translation="MKEIICAALLGMSTIAYAQDKNDLKLWYRQPAGGRWENALPIGN
                     GRLGAMVYGNVELENIQLNEHTLWSGSPNRNDYPEMLTALPEARRLIFEGKPAEAQKF
                     LEGSVKFTKSSGQMFEPVGSLRLAFDGQENFSDYRRELDISTGVTTTTYTVGGVGFTR
                     QVLASLPDRVIVVRLTASRPGSLSFRAYYTSPEKNAERKTRAGRNADRNTMAGKELVI
                     SGTTIDHEGVKGMVRYEGIAEIKAEGGTVNDTDTSLVVSGANAATIYISIATNFVNYH
                     DLGGDAHGRAEGWLGKAWPKSWAEIGKAHEAAFRRYFDRVRLDLGSAENDSLPTDERL
                     KNFRNVNDPSFVTLYYQYGRYLLISSSQPGGQPANLQGIWNDKLFPPWDSKYTLNINA
                     EMNYWPAEKTNLTEMHEPFLQMVRELSVTGRETARVMYGVRGWMAHHNTDIWRSTGPV
                     DGIFWGIWSDGGAWTSQHLWEHYLYDGDKNYLASVYPIMKGAAMFYVDDLVE"
BASE COUNT          758 a          861 c         1175 g          744 t
ORIGIN      
        1 cttccgcccg agttccacgc tgtgctggtc gatggtggaa agggagggtg tcacgagttg
       61 tgcgaaggtc tcatcggcaa agccgaaaac gcccagcccg gatgggatcg tgatgccgac
      121 ggaggcagcg gcatccatga cgccgagaga ctggtggtca gagaccgtaa agatagcgtc
      181 gggcctgttg ggcagcctga gtaacggcag cgccgcctgc tggccccgct cgcgggtgag
      241 gacattgtcg acgatgaaat tgtaggggat ggggaggttg gcttccgcga tggctgcaat
      301 gaaaccgtcc ttgcggtcct ggaaggcctt tacgcggacg gggccgccca gaaaggcgat
      361 gttccggtag ccctcgttga taaggcttct gacggcttca taggaaacag tcctgttgtc
      421 gttgacgacc ttgaaactgt cgaattcgtc caggcagcgg tcgaactgga tgacggggat
      481 cttgttcttc gtggcctggc ggagatgatc cgcgctggtc gtctccgctg cattggagat
      541 aaggatacag tctacgttct gatggatgag ggtcatgatg gccttgccct ctttttcaaa
      601 agactcgtgg gactggcaga tgacgaggct gtggttgtgg gcaaagcaga tctcttcgat
      661 gccggagatg gcgtcggaga aaaagctctg gttgatatgg gggaccacca cgccgatggt
      721 ccttgactgc ccccggcgca ggttggccgc atgggaattg tgcttatagt tcaactgttc
      781 cgccttgtcc tttacgcgcc gccggatgtc ttcgccgacc gacgggtggt tattgagcgc
      841 tttggacaca taggagggcg agatggccag ttcttttgca atgtcgtaaa tggtcgctct
      901 ttttcgacta ttcatttcaa catattcaac aatgaaataa agttgaattt acgcaataaa
      961 ttatgaacca aaggcaatgg agattaaatt accgcccgtg atattcggga ccagcggact
     1021 gggaaacctg tttgtcgccc tggacgaaga atcaaaaatg aaggtcgtgg aggcctgtat
     1081 cctgttgtcc ggcggacagg tggtattcga ttccgcgggt aagtatggcg ccgggctggc
     1141 gctggaagtg ctgggccgcg ttctgcgccg gctgaacatc gcccccgggg atgtcgtcat
     1201 cagcaataag ctgggctggg tacgcagtcc gctgcagggc gaggaaccga ccttcgagcc
     1261 gggtgtctgg aaggacctgc ggcatgatgc ggtgcagaag atcggctacg acgggatcat
     1321 agaatgtttt gaccagggta atgaattgct gggcggctat acgcccagga tggtgtcggt
     1381 gcatgatccg gacgagtacc tggcgggcgc cgggggccgg cggggactgc aagatcagcg
     1441 ttacgaggat atcctggatg gctatgaggc tttgttcgat ctgaaacgtc tgggccgcgt
     1501 atcggccgta ggcgtggggg ccaaggactg gcgggtgatc aaaaggatcg tccgggacgt
     1561 gccgctggat tgggtcatgt tcgccaacag catgacgctg aaaagtcatc catgggagtt
     1621 ggcctatctg ataaaggaac tggctgagag gggtgtgacg gtgatcaatt cggcggtgtt
     1681 ccattccggt ttcctggtag gctctgattt cttcgactac cggcggatat cgaggggtga
     1741 tcccgtcacc gatccgctgt ttcgctggcg ggaggagctc tttgccgtat gcgaagagtt
     1801 caatgtgaca ccggcggctg cctgtgtaca atttgcgctg ggggtgccgg gcgtcagcag
     1861 tattgcgctc aattcttcca atcccgaccg ggtaaaggaa aacctggagc tggctgtacg
     1921 gcggctgccg gacgagttct gggaggtgtt gagggaaagg gaactgatca gcttcgatcc
     1981 attggcggcg gtacggccgg gagacttaat taacaagtat actatttttc gatgaaggaa
     2041 atcatttgcg cagcattgct gggtatgtcg acgatcgcct atgcacaaga caagaacgat
     2101 ctgaagcttt ggtaccggca gccggcgggc gggcgctggg aaaatgccct accgatcggg
     2161 aacggccgtc tcggcgccat ggtctatggg aacgtggagt tggagaacat ccagctcaat
     2221 gaacatactc tatggagcgg aagccctaac cggaatgact acccggaaat gcttacggca
     2281 ttgccggagg cgaggaggct cattttcgag ggtaagcccg ccgaagctca gaaatttttg
     2341 gaaggatcgg tgaagttcac taaatcaagc gggcagatgt tcgagcccgt gggcagtctg
     2401 cggctggcat tcgacggcca ggaaaatttt tcggattacc ggcgggagct tgacatcagc
     2461 accggggtga caactactac ctatacggtg ggcggcgtcg ggttcacccg acaggtgctc
     2521 gcctccctgc ccgaccgggt gatcgtcgtc aggctgaccg caagcaggcc gggcagcctg
     2581 tctttccggg cctattatac gtcgcccgaa aagaatgcgg agcgcaagac gagggccgga
     2641 aggaatgcag atcggaatac gatggcgggt aaagaattgg tgatcagcgg aacgacgatc
     2701 gaccatgaag gtgtgaaagg aatggtccgg tatgaaggga tcgcggagat caaggcggaa
     2761 ggcgggaccg tgaacgatac agatacgtca ctggtggtca gcggggccaa tgcggccacg
     2821 atctatatat cgatcgctac caatttcgtc aattaccatg acctgggcgg tgacgcgcat
     2881 ggccgtgcgg agggatggct tggcaaggcc tggccgaaaa gctgggcgga gatcgggaag
     2941 gcgcatgaag cggctttcag gcggtatttc gaccgggtgc ggctggacct gggcagcgcg
     3001 gagaacgaca gcctgcccac ggacgagcgg ctcaagaatt tccgcaatgt caacgaccca
     3061 tcttttgtca ccctgtatta tcagtatgga cgctacctgc tcatttcttc ctctcagccg
     3121 ggcgggcagc cggccaacct gcagggtatt tggaacgaca agctctttcc gccgtgggat
     3181 agcaagtaca ccctgaatat caatgccgag atgaactact ggcccgcgga aaagaccaat
     3241 ctaacggaaa tgcatgagcc attcctgcag atggtgcggg aactgtcggt gacgggccgg
     3301 gagacggccc gcgtcatgta cggcgtccgg gggtggatgg cgcatcacaa tacggacatc
     3361 tggcggagca cgggaccggt ggacggcatc ttctggggca tctggagcga cggcggcgcc
     3421 tggacgagcc agcatctctg ggagcattac ctgtatgatg gcgataaaaa ttatctcgcg
     3481 tccgtgtatc ccatcatgaa gggagcggcg atgttctacg tggacgacct cgtggagg
//
LOCUS       DATGFG010000974         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_54396, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000974 DATGFG010000000
VERSION     DATGFG010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54396"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /locus_tag="VKQ52_18380"
     CDS             <1..1775
                     /locus_tag="VKQ52_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ89233.1"
                     /translation="YSRNFTGHPERFPNDGNGNMIFVPGIMHFSGPASLSYTNPLNIN
                     SASDIFYNPIEDVHITDASLALGFKGTLGDDWDWDLSNTAGGNNFHYFGDGTFNASLG
                     AAGATRNHFNDGGFYFFQNTTNLDLSRHYKDVAQGMTLAFGAEFRYEQYKIYKGEEAS
                     YSNYSTQTVAYPNGDTAYPATGAQGFPGFQPADEHKATRSNVAGYVDVAIDITKAWLV
                     DGAVRLENYSDFGFVNTYKLATRYKLPGNVNLRGSVSTGFRAPSLQQINFSNTESNVV
                     AGVPSLVKIAPNYGPITRAAGIPDLKQEKSVNASLGASWKPVRNFTFTVDGYWVKITN
                     RVVLSGTFQATDNTLDPGLTSTLQGLKVNSAQFFANAVNTSNIGLDVVADWNQTWGRN
                     RLSILFAGNVQHMNIDKINVPEKLNDTYLHQQTFFSGREQYFVKASAPPAKLSLNLQY
                     DMNKFTVGTHFTYFGQIKLEGYGYSGDNSLAGTGKQGDPDLIGTGITPVVNLGADQSG
                     PLVPEIFVYHGKVVSDLFFSYRFSKKVSLFWGIDNIFNVHPDLGIVPGANAQAFDGET
                     GGPWDAVQMGYNGMRPFAKLAVSL"
     gene            1892..2350
                     /locus_tag="VKQ52_18385"
     CDS             1892..2350
                     /locus_tag="VKQ52_18385"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLZ89234.1"
                     /translation="MKPSILLFSIFITLTASAQMPPANADHMKKYITDSTAIAGVLTD
                     IYFKGIYEGDVKLLESAYYDGTLLFGDVKDQPYYKTLAQYLDGVKNRQSPKDSGKPFS
                     GDIISIEIINSIAVAKVQVKMYDFNYHELLSFHRFGNKWLIVNKMISDVQ"
     gene            2340..>2697
                     /locus_tag="VKQ52_18390"
     CDS             2340..>2697
                     /locus_tag="VKQ52_18390"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ89235.1"
                     /translation="MSSNKVWYVTGASQGLGLTLVRQLLSAGYRVAATSRSATDLQKA
                     VGAIDRDRFLPLAVDLTSADAIRDSIEQTVANFGTLDVIVNNAGYGMEGTVEEVEEEK
                     VRAIFGINVFATINVTK"
BASE COUNT          631 a          841 c          676 g          549 t
ORIGIN      
        1 cctactcccg gaatttcacc gggcacccgg agcgtttccc caatgacggc aacggcaaca
       61 tgatcttcgt accgggtatc atgcattttt ccggtcccgc ttctttgtcc tacacaaacc
      121 cgctgaacat caattcggcc agcgatatct tttacaaccc catcgaggac gttcacatca
      181 cggacgcctc actggccctc ggcttcaaag gaacccttgg cgatgactgg gactgggatc
      241 tgagtaacac cgccggcggc aataacttcc actatttcgg cgacggcacc ttcaacgcct
      301 ccctgggagc ggccggtgcg acccggaacc atttcaatga cggcggcttt tatttcttcc
      361 agaatacgac caacctggat ctctcccggc actacaagga cgtggcgcag ggcatgaccc
      421 tagcctttgg cgcggaattc cgctatgagc aatacaagat ctacaaggga gaggaagcct
      481 cttattcgaa ttacagcacc caaaccgttg cctatcccaa tggcgacaca gcctatcccg
      541 ctaccggcgc ccagggattc cccggcttcc agccggcgga tgagcacaag gccacccgct
      601 ccaacgtagc cggctatgtg gatgtggcga tcgacattac aaaggcctgg ctggtggacg
      661 gcgctgtccg gctcgagaac tattccgact ttggattcgt caacacctat aaattggcca
      721 cccgctacaa actccccggt aatgtcaacc tcaggggttc cgtaagcacg ggcttccgcg
      781 ccccttccct gcaacagatc aatttcagca ataccgaaag caatgtggtg gcgggagtcc
      841 cttccctggt gaagatcgcg cccaactacg gccccatcac ccgggccgcc ggcatccccg
      901 acctgaaaca ggagaaatcg gtgaacgcca gcctgggcgc ttcctggaag ccggtgagga
      961 atttcacctt caccgtggac ggctactggg tgaagatcac caatagggta gtgctctccg
     1021 gcaccttcca ggccaccgac aataccctcg atccgggtct gaccagcacg ctccagggtc
     1081 tgaaggtcaa cagcgcgcag ttcttcgcca atgccgtcaa cacctctaac atcgggctgg
     1141 acgtggtcgc cgactggaac cagacctggg gccggaacag gctcagcatc ctgttcgccg
     1201 gcaatgtcca gcatatgaat atcgacaaga tcaatgtccc ggagaaactg aacgatacct
     1261 acctccacca gcagaccttc ttcagcggaa gggaacagta ttttgtaaaa gcgtcggctc
     1321 cccccgcaaa gctcagcctt aacctccagt acgatatgaa caagtttacc gtcggtacgc
     1381 atttcaccta cttcgggcag atcaaactgg aaggctatgg atattcaggc gacaattcac
     1441 tggccggcac cgggaagcag ggcgatcccg acctcatcgg tacagggatc acgccggtcg
     1501 tcaacctcgg cgcggaccaa tccgggccgc tggtgcccga gatcttcgtc tatcacggca
     1561 aggtcgtatc cgacctgttc ttctcctacc gcttctcgaa aaaggtgagc ctcttctggg
     1621 gaatagacaa catcttcaat gtacaccccg atctgggtat cgtacccggc gccaacgccc
     1681 aggccttcga cggcgaaaca ggcggcccct gggatgcggt gcagatgggc tacaacggca
     1741 tgcggccctt cgcaaaactg gcggtgagtc tctagatcca tcgccggctg atcctttgat
     1801 gacaaaatgt gatccaggtc acgtaattcc ctttcctttc cgtcgcatct ttgtgtggtc
     1861 gcagatctca aacaggtaaa atcacatggc catgaaacca tccatcctgc tattctcgat
     1921 ctttatcacc ctgacggcat ccgcacagat gccgccggca aacgcagatc acatgaaaaa
     1981 gtacatcacg gattccaccg ccatcgcggg agtcctcact gacatttatt tcaagggcat
     2041 ttatgaggga gatgtcaaac tcctggaaag cgcttactat gacggcaccc tgctcttcgg
     2101 ggacgtcaag gaccaacctt attacaagac cctggctcag tacctcgacg gcgtaaagaa
     2161 tcgccagagc ccgaaggact cgggcaaacc cttcagcggg gacattatct cgatcgagat
     2221 catcaactcc atcgccgtgg ccaaagtgca ggtaaagatg tacgatttca actaccacga
     2281 gctgctgtcc tttcaccgct tcgggaacaa atggcttatc gtaaataaaa tgatatccga
     2341 tgtccagtaa taaggtttgg tatgtcaccg gggcctccca gggattggga ctcacccttg
     2401 tcaggcagct gctttccgcc ggctaccgcg tagccgccac atcgcggtcc gccaccgacc
     2461 tgcaaaaagc cgtcggcgcc atcgatcgcg accggtttct tcccctcgcg gtggacctca
     2521 ccagtgcgga cgctatccgc gactccatcg aacaaaccgt tgccaacttc gggaccctcg
     2581 atgtcatcgt caacaatgcc ggttatggga tggaaggcac ggtcgaggag gtggaagaag
     2641 aaaaggtccg cgccatcttc gggatcaacg tcttcgctac gatcaacgtc acaaaat
//
LOCUS       DATGFG010000975         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1424097, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000975 DATGFG010000000
VERSION     DATGFG010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424097"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VKQ52_18395"
     CDS             <1..404
                     /locus_tag="VKQ52_18395"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLZ89236.1"
                     /translation="TLLAPAGFLLKKTDAGRAWIGGSARTFSEKEGLIIEANTLRGTP
                     LYDAGLDIDDKILAIDNQDTRTLGDLNAVLGKLHPGAAVGIRYLHRGEEKKGTITAQE
                     NPAVSVITFEQAGQPVTPAIQQFRKNWLGAK"
     gene            412..837
                     /locus_tag="VKQ52_18400"
     CDS             412..837
                     /locus_tag="VKQ52_18400"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ89237.1"
                     /translation="MSIRLAKNTDAPDLSRLLTQLDYPGTETFIAAKLGKILADPAER
                     LLVWAEGEHVLGFLSLHFHNQLGTEGDFAIISYFAVDEAAQSKGIGAQLEAAATRLAQ
                     EHGCDRIVVHCHTRRTRAHAFYYRQGYEESPKYLIKRLS"
     gene            896..1363
                     /locus_tag="VKQ52_18405"
     CDS             896..1363
                     /locus_tag="VKQ52_18405"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HLZ89238.1"
                     /translation="MTTLQQIRDWGATHHPRWLVIVRVGLGLFLFAKGITFMQNATLL
                     DQLIYGRASLAEDQTHWLPILITWANLLTGLLMIIGLWTRLMALLQVPILIGAIIFII
                     AQKGGSVPESEMLLAILTLLLMIFFLVEGSGPLSLDTYLEKNRGSGSQGRNLP"
     gene            complement(1370..>2368)
                     /locus_tag="VKQ52_18410"
     CDS             complement(1370..>2368)
                     /locus_tag="VKQ52_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89239.1"
                     /translation="SDAGKKPDSSKVAAKAAVATDTVPAPPPAEAAGPLDTALYNKLM
                     HHLANRDSSGRWPAKTVYPNPGAVLPFKRIIAYYGNLYSKNMGILGEYPPDEMLRRLK
                     IELKKWQEADTSIPVVPALHYIAVTAQGSPGKDGKYRARMPFKQIDSVLHIAARINAL
                     VFLDVQVGFSNVEAEIPLLEKYLKMPNVHLGIDPEFSMKSGKRPGTVIGTMDASDINF
                     CSDYLTRLAKENNLPPKILVVHRFTKNMVTNTQKVHVSKEVQVVLDMDGWGPVSLKTD
                     SYRMWVAAYPIQFTGFKLFYKNDVRKPRGVNMKETLMMTPAQVLAQKPSPIYIQYQ"
BASE COUNT          527 a          730 c          608 g          503 t
ORIGIN      
        1 ataccctcct cgcacccgcg ggcttcctcc tcaaaaagac cgacgccggc cgggcctgga
       61 tcggcggcag tgcccgcacc ttctcggaaa aagaagggct catcatcgag gccaatacgc
      121 tccggggtac ccccctctat gacgccggcc tcgacatcga cgacaagatc ctggccatcg
      181 acaaccagga tacccgaacg cttggcgatc tgaatgccgt cctcggtaag ctccacccgg
      241 gcgcggccgt cggcatccgc tacctgcatc gcggcgagga aaagaaaggg accatcaccg
      301 cccaggaaaa tcccgccgta tcggtcatca ctttcgagca ggccggccag ccggtaaccc
      361 cggccatcca gcagttccgt aagaactggc tcggcgctaa ataaaccgac catgagtatc
      421 cgcctggcca aaaacactga cgccccggac ctctcccgcc tgcttaccca gctggactat
      481 cccgggacgg agacgttcat agccgcaaaa ctggggaaaa tactggccga cccggccgaa
      541 cgattgctgg tatgggccga gggagagcac gtactcggct tcctctccct gcatttccat
      601 aaccagctgg ggacggaagg cgacttcgcc atcatcagct actttgccgt ggacgaggcc
      661 gcccagagca agggcatcgg cgcgcaactc gaagcagcag ccacccggct ggcacaggaa
      721 catggctgcg accggatcgt ggtccactgc cacacccgcc gcacgcgggc ccacgcgttt
      781 tactaccgcc agggctatga agaatctcct aaatacttga ttaaaaggtt gtcatgaagg
      841 catagctatt gcaacgccta gtagtagtaa ctttactgtc taaaaaacaa ttactatgac
      901 tacgttgcag caaatacgtg actggggagc cacccatcat ccgcgatggt tggtcattgt
      961 ccgcgtcgga ctgggcttat ttctttttgc aaagggaatt acttttatgc agaatgctac
     1021 cctgctggac cagctcatat acggccgtgc atccctggct gaggaccaga cccactggct
     1081 gcccatcctt atcacctggg ccaatctcct gacaggactc ctgatgatca tcgggctctg
     1141 gacccggctg atggccctgc ttcaggtgcc gatcctcatc ggcgcgatca tcttcattat
     1201 tgcacaaaaa ggcggatcgg taccggaatc cgaaatgctc ctggccatcc tcaccctgtt
     1261 actgatgatt tttttcctgg tagagggcag tggaccactc tcactcgaca cctacttgga
     1321 aaagaatcgc ggtagcggct cccagggacg caacctgcca taatcccccc tattgatatt
     1381 gtatgtagat cgggctcggc ttctgcgcca acacctgggc gggagtcatc atcagggtct
     1441 ctttcatatt gaccccacgc ggcttgcgca catcgttctt gtaaaacagc ttaaacccgg
     1501 tgaactggat tggataagct gccacccaca tccgatagct gtcggtcttt aggctgacag
     1561 gaccccagcc atccatatcc agcaccacct gcacctcctt gctcacatgc accttttggg
     1621 tattggtgac catattcttg gtgaagcggt gcacgaccaa tatcttgggc gggagattat
     1681 tttcctttgc cagcctggtc aggtaatccg aacaaaaatt gatgtcgctg gcatccatcg
     1741 tgccgataac cgtacccgga cgttttcccg acttcatgga gaactcggga tcgatgccca
     1801 ggtgaacatt gggcatcttg agatatttct ccagcaaagg gatctcagcc tcgacattgc
     1861 tgaaaccgac ctgtacgtcc aggaatacca gcgcattgat ccgcgcggca atatggagga
     1921 ccgaatcaat ctgcttgaaa ggcattcggg cccggtattt tccgtccttg cccgggctcc
     1981 cctgcgccgt tacggcgata taatgaagcg ccggcactac cggaatgctg gtgtctgctt
     2041 cctgccattt cttcaattct attttcagcc ggcgcaacat ctcgtccggc ggatactcac
     2101 cgaggatgcc catattcttc gaatacaggt taccataata cgcgattatg cgcttaaagg
     2161 gcaggacggc ccccgggttg ggatataccg tcttcgccgg ccaacgaccc gaagaatcac
     2221 ggttggccaa atgatgcatc agcttattgt ataaagccgt atccagcggc cccgcagcct
     2281 cggccggcgg cggcgcggga acggtatcgg ttgcaacagc agccttcgcg gccaccttgg
     2341 aggaatcagg cttcttaccg gcatcgga
//
LOCUS       DATGFG010000976         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_670382, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000976 DATGFG010000000
VERSION     DATGFG010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670382"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VKQ52_18415"
     CDS             complement(<1..911)
                     /locus_tag="VKQ52_18415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLZ89240.1"
                     /translation="MMSIIPGLDNGQPSPARHRLFLLKQLEFNMEANLLAKYNQPIPR
                     YTSYPTVPFWKEDADTGCWENDFIGAYNGHNGKEGISLYLHLPFCESLCIYCGCNKKI
                     TTNHKVEEEYLGAILKEWSMYRKMMEHPPVIREIHLGGGTPTFFSPENLHRLLSTILA
                     GAVIHPDHAFSIEGHPNNTTRGHLDTLYALGFRRISYGVQDLNPEVQRAIRRWQPFEK
                     LSEATETARAAGFSAVNFDLIYGLPLQTPERLRETIRQSVSLRPDRIAFYSYAHTPWA
                     NCSQRLIDMAQLPSPSDKLYLHQLGKML"
     gene            961..1731
                     /locus_tag="VKQ52_18420"
     CDS             961..1731
                     /locus_tag="VKQ52_18420"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89241.1"
                     /translation="MLLPTQFRQFLEETGAIARFSGRYFSQGTRPRWEFSELIRQCYI
                     IGYKSFPLVGLTGFIMGLVLTMQLRPSLVNYGVESQLPVMVGIAIVREIGPVITALIF
                     AGKIASSMGAELGSMKVTEQIDAMEVSGTNPFKYLVVTRVAASTLMLPVLVLLGDGIS
                     LLGAWIGVNIRSVTSFDLFFRQVVDSFSFGDVVPAFIKTYFFGFAVGVIGCYKGYTSS
                     KGTEGVGKAANSAVVLASILIFLIDLVVVQVTDVLGLN"
     gene            1737..2513
                     /locus_tag="VKQ52_18425"
     CDS             1737..2513
                     /locus_tag="VKQ52_18425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ89242.1"
                     /translation="MEKSYPDIETKEPVAAQDPVIVIEHLCKRFGDNIVLNDFNLRVG
                     REENVVVLGKSGVGKSVLIKCIIGLLIPDSGSIRVFGQDVTKLGHNELDRVRAKVGFL
                     FQSNALYDSMTVRKNLEFPLRRHWIRISQEEVDAMVMEVLENVGLADTVDKLPEELSG
                     GMRKRIALARTLILKPEIILYDEPTTGLDPVTGREISHLIVEMQKKYHTASIIISHDM
                     HCVRLTADRVALLMDGRCYAEGSLDDLAHSTDKNILQFFE"
     gene            2523..3494
                     /locus_tag="VKQ52_18430"
     CDS             2523..3494
                     /locus_tag="VKQ52_18430"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLZ89243.1"
                     /translation="MGSGSKNIRLGLLVLAGMTLLISTLYVIGKNQNLFGSHFALRAR
                     FRNINGLMKGNNIRFSGIQAGTVSDVRVLNDTAIEVVLLVDQSMKPFIHRNALAAIGT
                     EGLIGNKVVNIIPVNDPAPLAEDGDILPIQKAVSTDEMLLTLDKTNRNIAEITDGLKV
                     TVARVNGSVALWGLLNDRRMAGDVRASLEHIREASVEAEEMTKGLRGLVEQVGSGKGS
                     MGRLLVDTVFAAELSGAVEKIRQAGDRLGTAGQQAGELAASAKNLVEDSALQARLQAS
                     MENIRKGTEAFSQDMEALKHNFLLRGYFRRQEKKNRQLQKQGRDTTK"
     gene            3566..4369
                     /locus_tag="VKQ52_18435"
     CDS             3566..4369
                     /locus_tag="VKQ52_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89244.1"
                     /translation="MKIAVWGLTLSCLSAFYLLSCTKHSDSAAGASAGSHTGARTGTL
                     VFSGSKVQKGQPLLVSLSGGTSTAIRWSVAANPGNVHVVAAKDQAMILFARPGTYRVT
                     ASSLGADSSATDSSWGTISVTDTVYQPVPPPAYDTTTLAGDQITITPSVDSNANLFLL
                     AQTKNSYGCASTLIYEMSAGGTGGGGIGLTFYEVVSNGTGPCNGVQNPASAFLFPGAT
                     STWPIGTYPVTARLNGITYTGTLTITNTTYTFSWNYSSGVLISPLQLNK"
     gene            4435..>4846
                     /locus_tag="VKQ52_18440"
     CDS             4435..>4846
                     /locus_tag="VKQ52_18440"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLZ89245.1"
                     /translation="MVETEALQQLISGCARNDRASQEKLYKQFYAPLFSLCRRFFQND
                     HEAIESVNDGMLKVFEKIGSYREGKGRFFNWMYTIVRNTAVDKFRASVPIPVIGLQEP
                     EEAIPEPDIGGNPGAWLEQKELALLFDRLSPAPRG"
BASE COUNT         1044 a         1169 c         1537 g         1096 t
ORIGIN      
        1 agcatttttc ccagttgatg aaggtagagt ttatcgctgg gggagggcag ttgcgccatg
       61 tcgatgaggc gttgcgagca gttggcccag ggagtgtggg cgtagctata gaaggcgatg
      121 cgatcggggc gcaggcttac cgattgccgg atcgtttccc gtaacctttc cggggtctgc
      181 aggggcaggc catagatgag atcgaaattg acggccgaga aaccggcggc ccgggccgtt
      241 tctgtcgctt cgctgagctt ttcaaagggt tgccagcgac ggatcgcccg ttgtacctcg
      301 gggttcagat cctgtacacc gtagctgata cggcggaagc cgagggcgta cagggtgtcc
      361 aggtgcccgc gggtagtatt gttgggatgg ccttcgatgc tgaacgcatg atccgggtga
      421 atgaccgccc cggcgaggat cgtcgacagc aggcgatgga gattttccgg tgagaagaag
      481 gttggagtac cgcctcccag gtggatctcg cggatcacag ggggatgctc catcattttt
      541 ctatacatac tccattcttt tagaatggcg ccgaggtatt cctcttccac tttatgattg
      601 gtcgtgatct tcttgttaca gccgcaatag atacaaaggg attcgcagaa aggaaggtgc
      661 aggtagaggc tgatgccttc cttcccgtta tggccgttgt atgcaccgat gaaatcattt
      721 tcccagcagc cggtgtcggc gtcctctttc cagaagggga cggtgggata gctggtatag
      781 cgggggatcg gctggttgta tttagccagt aaatttgctt ccatattgaa ttcgagctgt
      841 ttcaacaaaa ataaccggtg gcgggccggg gacggctgac cgttgtcaag gccggggatg
      901 atgctcatca tggacgggag gcttttgccg gcgtagtttc atccccgtaa ttcaaaaaag
      961 atgcttttac ctacgcaatt ccggcagttt ttggaggaga caggggcgat cgcccggttt
     1021 tcgggaaggt atttctcgca gggcacccgt ccccggtggg aattcagtga actgatccgc
     1081 caatgttata tcatcgggta caagtccttt ccccttgtcg ggctgacggg ttttattatg
     1141 gggctggtgc ttaccatgca gttgcggcct tcgcttgtca attatggcgt tgaatcgcaa
     1201 ttgccggtaa tggtagggat cgccatcgtg cgggagatcg ggccggtgat cacggcgttg
     1261 atctttgccg ggaagatcgc cagcagcatg ggggccgaac tggggtccat gaaggtgacc
     1321 gagcagatcg atgcgatgga agtaagcggt acgaatccct ttaaatacct ggtggtgacg
     1381 cgggtggctg cgtcgacgct gatgctcccg gtgcttgtgt tgctggggga tggcatttct
     1441 cttttggggg cctggatcgg cgtgaatatc cgcagcgtga ccagctttga cctgtttttc
     1501 cggcaggtgg tggacagttt cagctttggc gatgtggttc cggcctttat caagacctat
     1561 ttctttggtt ttgccgtagg ggtgatcgga tgttacaagg gatatacgtc gagcaagggc
     1621 accgagggtg tcggcaaggc cgccaattcc gccgtggtgc tggcttcgat cctgatcttc
     1681 ctcatcgacc tggtcgtggt gcaggtgacg gatgtgctgg gattaaattg actatcatgg
     1741 aaaagagtta ccccgatatc gaaacgaagg aaccggttgc cgcgcaggac ccggtgatcg
     1801 tcatcgagca cctgtgcaag cggttcgggg ataatatcgt gctgaatgat ttcaacctcc
     1861 gggtgggaag ggaagagaat gtggtagtgc ttgggaaatc cggggtcggc aagtcggtgc
     1921 tgatcaaatg cattatcggg ttgctgatac cggacagcgg cagcatccgc gtgtttgggc
     1981 aggatgtgac gaagcttggt cataacgagc tcgaccgggt gcgggcaaag gtgggttttc
     2041 ttttccagag caatgccctc tatgattcga tgacggtgcg caagaacctc gagttccccc
     2101 tgcggcggca ctggatcagg atctcccagg aggaggtgga tgcgatggtg atggaggtgc
     2161 tggagaatgt gggcctggcc gacacggtcg acaagctgcc ggaggaattg tccggtggga
     2221 tgcggaagcg gatcgcgctt gcccggaccc tgatcctgaa gccggagatc atcctgtacg
     2281 atgagccaac cacggggctg gacccggtga cgggccggga gatatcccac ctgatcgtcg
     2341 agatgcagaa aaaatatcac accgcgtcga tcatcatttc tcatgatatg cattgtgtgc
     2401 ggctgaccgc ggaccgggtg gctttgttga tggatggacg atgttatgcg gagggttcgc
     2461 tcgacgacct ggcgcattcg actgacaaaa acattttaca gtttttcgaa taaatctctt
     2521 gtatgggatc tggcagtaaa aatatacgat tggggttgct ggtgctggcg gggatgaccc
     2581 tgctcatctc caccctctat gtcatcggca agaaccagaa cctcttcggt tcgcattttg
     2641 cgttgcgggc gcggttccgt aatatcaatg gcctgatgaa gggcaataat atccgtttct
     2701 ctggtatcca ggccggtacg gtcagtgatg tccgggtgct gaacgacacc gccatcgaag
     2761 tggtgctgct ggtcgatcag tcgatgaagc cgttcatcca ccggaatgcg ttggcggcca
     2821 tcggaacgga aggccttatc ggcaacaagg tggtcaatat catcccggtg aatgacccgg
     2881 cgccattggc ggaggacggg gatattcttc ctattcagaa ggcggtgagc accgacgaga
     2941 tgctgctgac cctggacaag acgaacagga atatagcgga gatcaccgac gggttgaaag
     3001 tgacggtggc gcgggtcaac ggcagtgtgg ccctttgggg gctgttgaac gaccgccgga
     3061 tggcggggga tgtgcgggct tcgctggaac atatccggga agcgagtgtc gaagccgagg
     3121 aaatgacgaa aggactgcgg ggactggtag aacaggtggg cagcggcaag gggtcgatgg
     3181 gcaggctgct ggtggatacg gtatttgccg cggaattatc gggtgcggtg gaaaagatcc
     3241 ggcaggcggg agaccggctg gggaccgcgg ggcagcaggc gggggaattg gcggcaagcg
     3301 caaagaacct tgtggaagac agcgcgcttc aggcaaggct gcaggcgagc atggagaata
     3361 tccggaaggg tacggaggcg tttagccagg acatggaagc gttgaagcat aattttttac
     3421 taagaggata tttccgtcgc caggagaaaa agaaccggca gttgcaaaag caggggaggg
     3481 acaccactaa ataattttta tttttatttt accccaacat tcgggatgcc cggctacgta
     3541 tagtagctga accaacacct cttttatgaa aatagctgta tggggactca ccctctcctg
     3601 cctatctgcc ttttatcttc tttcctgcac caagcatagc gattcggccg cgggcgcgtc
     3661 tgccggcagt cataccggag ccaggacagg aacactggtc ttttcgggaa gcaaagtaca
     3721 aaaaggtcag ccgctgcttg tttcgttgtc cggcggcact tccaccgcga tacgctggtc
     3781 ggttgcggcg aaccccggca atgtacatgt tgtagcggca aaggaccagg ccatgatcct
     3841 ttttgcgcgg ccgggtacct accgggtgac cgcctcctcg ctaggagccg acagttcggc
     3901 aacggatagc tcctggggga cgatctccgt aacggatacg gtgtatcagc cggtgccacc
     3961 cccggcgtat gataccacga cgcttgccgg cgaccagatc acgatcacac cctcggtgga
     4021 ctccaatgcc aacctgttcc tgctggcaca aacaaagaac tcctacggct gtgcgtcgac
     4081 gttgatctac gagatgtccg ccggcggtac cggagggggt ggcatcggcc ttacgttcta
     4141 cgaagtggtc tccaacggaa ccgggccgtg caatggggtg cagaacccgg caagcgcctt
     4201 tctgttcccc ggcgctactt cgacctggcc catcggcacg tatccggtga cagcacggtt
     4261 gaatggtatt acctatacgg ggacgctgac gatcacgaat accacgtata ctttcagttg
     4321 gaattatagt tccggggtac ttatttcgcc cttgcaacta aataaataat gagatcaata
     4381 acgccgaatc aggatcccgg ggcaaccgcc ccgggaattt ggcgtatata accgatggta
     4441 gaaacggaag cgctgcaaca gttgatcagc ggttgcgccc ggaacgacag ggcaagccag
     4501 gaaaaattgt acaaacaatt ttatgcgccc cttttttccc tatgccgcag gttctttcaa
     4561 aacgatcatg aggcgataga gtccgtcaac gacgggatgt taaaggtatt cgagaagatc
     4621 gggagttata gggaaggcaa gggtaggttc tttaactgga tgtatacgat cgtgcgaaat
     4681 acggcggtgg acaaattcag ggcttctgtc ccgatacccg ttatcggcct gcaggaaccg
     4741 gaagaagcca ttcccgagcc ggatatcggc ggcaatcccg gcgcctggct ggagcaaaag
     4801 gagctcgctc ttcttttcga caggcttagc ccggcgccga gaggca
//
LOCUS       DATGFG010000977         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1653006, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000977 DATGFG010000000
VERSION     DATGFG010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653006"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VKQ52_18445"
     CDS             complement(<1..349)
                     /locus_tag="VKQ52_18445"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLZ89246.1"
                     /translation="MSEEKEITIYDIARLLGISASTVSRGLQNHPAVNIKTRKRIADT
                     AAKLGYRSNTFATSLRKQKTRTIGAIIHELHSEFVISALAGIEKITKAAGYDLLIGHS
                     SETMAKEVANAHNL"
     gene            756..3758
                     /locus_tag="VKQ52_18450"
     CDS             756..3758
                     /locus_tag="VKQ52_18450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89247.1"
                     /translation="MHLLSRMVRRMAVCLAALAFFYAGTASAQGSLKVSGKVTDNNAT
                     PLPGVTVTIKGTTTSVVTGNDGVFTITVPGASSVLAFSYVGYQQQEAPVGSRTAFDIR
                     LAPLNSNLNEVVVVGYGTQKKREITSAITRVDAEQFNKGNISDVSQLLQGKVAGLSIS
                     RPGGDPNAGFTMRLRGLSTLGANTSPLIVIDGQIGADINSVDPNDIASMDVLKDGSSA
                     AIYGTRGSAGVIIITTKSGSRSGPQVSYNGSVTAEKGARFTKHMTAAEFRALLQTSGA
                     GTDYGASTDWNKAITRTGITQDHNLSLSGGSQHTSYNVSLNYRSGEGIAITTGFNQLN
                     GRLNLTHKAMNDKLVFNVQASSTHRNSQVGFSNAFEYATIYNPTSPIHVPVGSANDLA
                     GGGWFEVQATDYSNPVAMLVQNSNNVELKRQNIAASAEYEFLPGLKFLVRYAQQTTSS
                     HTDKYSPITAFANRGFDAALDNGYGRHGYKILQDDESVNQLYENTLSYQHKVSDFDLG
                     AVAGYSYQDFTNNGSYIGAGNFVTDAASGNIAAALDIPYGLATISSYKNGSKLVAFFG
                     RVTANYKNMAFLAASLRREGSTQFGPNNKWGNFPAVSGGVDFSHIWEIPSVNSLKLRA
                     SYGQTGSLPPYSYLSLPTLTVSGSYYAGNDQYGFTYAPNQNPNPNLKWETKAETDIGL
                     DFSVLNNRLSGTVDYFRRTTSNLIFNATVPVPPNLVSTTWENIGTLQSSGFEFLINYD
                     MLRSRRFSWSTSVNFARAHVVLKSLDPTLAGSYVGASNLGSPGQEATQITRAMQGQPI
                     GLFYQAVYRGVDKNGKYLFDDGKGNAVADNNNYRTIIGNGLPKFEMGWTNTFRYDKWD
                     LNVFFRGAFGHDLINTYRAFYENPTVSSVYNVVKTKYYNPNVKDAQLFSSLFVEKGTF
                     VKLDNATIAYNWLLKKDGGPSGGFRSLRVYVTGYNLFLITGYTGADPEVRYQDQSTTP
                     PNILAPGIDRRENWVLTRSFTLGANLGF"
     gene            3784..>3985
                     /locus_tag="VKQ52_18455"
     CDS             3784..>3985
                     /locus_tag="VKQ52_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89248.1"
                     /translation="MKISLIIRLSVLVMLITVGSCTKLDETKYLYDTVNADGFYQTDA
                     ELSSAVGAAYANLTSVAGNNHYL"
BASE COUNT          872 a          967 c         1164 g          982 t
ORIGIN      
        1 agaggttgtg ggcgttggcg acttcttttg ccattgtttc ggaggagtgg ccgatgagga
       61 ggtcgtatcc tgcggccttg gttattttct cgatgccggc gagtgcggag atgacgaatt
      121 cgctatggag ttcgtggatg atggcgccga tggtgcgggt cttttgtttc cggaggctgg
      181 tggcgaaggt attgctgcga tagcccagtt tggcggcggt gtcggcgatg cgtttgcggg
      241 tttttatatt aacagcggga tggttctgca agccacggct tacggtagag gctgagatgc
      301 ccagcagcct ggcgatgtcg tagatggtta tttctttttc ctccgacacg gatataacct
      361 tttaattacc aaaagataag gcgtttggcg gaaggagggg aaaaaattta tttttgcaac
      421 cgattgtttt ttcgctaaaa ctttctttac ttttaacgta caattaacta tttgctgcta
      481 gttggcgtgc ccgggcgaat aaatgtcgtt ttaacatgat tccccggcgg ccggttggcg
      541 gatgatacgc ttgcttttta ttgttcaacc gtaacgattg ctttatgagc catttaaaac
      601 atcgattcgt tttactcccg ctttttttcc catttcctac tactttttcg agttcctgat
      661 cccggggttt ctctccgcgg aaggaggtct ttttttgcta aaaaatgcaa ccgattgtat
      721 tattaaattc tgattaatca accaaactta agcttatgca cctgctctca cgaatggtta
      781 ggaggatggc ggtctgttta gccgccctgg ctttttttta tgccggcacg gcgtcggctc
      841 aggggtctct caaggtgtcg ggaaaggtga ccgacaataa tgcgactccg ctgcccggcg
      901 taacggtgac gatcaagggg acgacgactt ccgttgttac gggtaatgac ggagtattta
      961 cgatcacggt acccggggcg tcctcggtac ttgccttcag ctacgtcggc tatcagcaac
     1021 aggaggctcc tgtggggagc cggaccgctt ttgatatccg gctggccccg ctgaattcca
     1081 atttgaatga ggtggtggta gtcggttatg gcacccagaa aaagcgggag atcaccagtg
     1141 cgatcacgcg ggtggatgcc gagcagttca ataaaggaaa catcagtgat gtctcgcagt
     1201 tgctgcaggg taaggtagcg gggttgtcga tctcgcggcc tggtggtgat cccaacgccg
     1261 ggtttacgat gcggcttcgg gggttgtcca ccctgggggc gaatacttcg ccgctgatcg
     1321 tgatcgacgg gcagattggc gcggacatca attcggtgga tccgaacgat atcgcgagca
     1381 tggatgtgct gaaggatggt tcttcggcgg cgatctatgg tacgcgtggt tcggcggggg
     1441 tgatcatcat cacgaccaag tcgggatcgc gttcgggtcc gcaggtgtcg tataacggga
     1501 gtgtgacggc ggagaagggg gccaggttca ccaagcatat gacggctgcg gaattccgag
     1561 ccctgttgca aacttcgggg gcgggtacgg actatggcgc aagtacggac tggaacaagg
     1621 ccattacgcg gacgggcata acgcaggatc acaacctctc gctttcgggt ggcagccaac
     1681 atacctctta taatgtctcg ctcaactacc gcagcggtga gggaatcgcc attaccacgg
     1741 gtttcaacca gttgaacggc aggctgaacc ttacccacaa ggcgatgaat gataagttgg
     1801 tcttcaatgt gcaggcgagc agcacgcacc gcaattccca ggtaggcttt tccaacgctt
     1861 ttgagtatgc gacgatctac aatcctacat cgccgataca tgtgccggtg ggcagcgcca
     1921 acgatcttgc cggcggcggc tggttcgaag tgcaggccac ggattattcc aacccggtgg
     1981 cgatgctggt gcagaacagc aataatgtcg aattgaagcg gcagaatatt gcggcttcgg
     2041 cggaatatga attcctgccg gggctgaagt tcctggtgcg gtatgcacag cagacgacca
     2101 gcagccatac cgataaatat tcgccgatca ccgcatttgc caaccgcggc tttgacgccg
     2161 cgctggacaa tggctatggc cggcatggct ataagatcct gcaggatgat gaatccgtga
     2221 accaattgta tgaaaatacg ctgtcttacc agcataaagt gagcgacttc gatcttgggg
     2281 ccgttgccgg gtattcttac caggatttta cgaacaatgg cagctatatt ggtgcgggca
     2341 actttgtcac cgatgccgcc agcgggaaca ttgccgccgc gcttgatatc ccttatggac
     2401 tggctacaat cagcagctac aagaacggca gtaagctggt cgcgttcttc ggccgggtga
     2461 ctgccaacta taagaatatg gcgtttttgg ccgccagtct tcgccgggag gggtcgacgc
     2521 agtttggtcc caacaacaag tggggtaatt tccctgcggt gagcggcggg gtggatttca
     2581 gtcatatctg ggagatcccg tcggtgaaca gtctgaagct gcgggcaagt tatggacaaa
     2641 cgggatcctt gccgccttac tcttatctgt ccctcccgac cctgacggtg tccggctcct
     2701 attatgcggg gaatgaccag tatggtttca cctatgcacc gaaccagaac cccaacccga
     2761 acctgaaatg ggaaaccaag gcggaaacgg atatcgggtt ggatttttca gtactcaaca
     2821 accggctgag cggtacggtg gattatttcc ggcggacgac ctccaacctg atcttcaatg
     2881 ccacggttcc cgtaccgccg aacctggttt ccacgacctg ggagaatatc ggaacgctgc
     2941 agagcagcgg ttttgagttc ctcatcaatt atgatatgtt gcggagccgg agattctcct
     3001 ggagcaccag cgtcaatttt gcccgggcgc atgtggtgtt gaaatcgctg gatcccaccc
     3061 tggcggggag ttatgtgggg gcgtccaacc tgggtagtcc cgggcaggag gccacacaga
     3121 tcacgagggc catgcagggg cagccgatcg ggctcttcta ccaggctgtt taccggggtg
     3181 tcgataagaa tggcaaatac ctgtttgatg atgggaaggg gaatgctgtt gcggacaaca
     3241 acaactacag gacgatcatc gggaacggcc tgccgaaatt cgagatgggt tggaccaata
     3301 ccttccgtta tgacaaatgg gatctgaacg ttttcttccg cggggctttt ggtcatgacc
     3361 tgatcaatac ctaccgggct ttctatgaga acccgacggt gtcttccgta tacaatgtgg
     3421 tcaagacgaa gtattacaat cccaatgtca aggatgccca gctgttcagc agcctgttcg
     3481 tggagaaggg aacctttgtg aagctggata acgcgaccat cgcctacaac tggctgttga
     3541 agaaggatgg tggtccgtcg gggggcttcc gcagcctgcg ggtatatgtc accggttata
     3601 acctcttcct tatcaccggg tataccggtg ccgacccgga ggtgcggtat caggatcaga
     3661 gtacgacgcc gccaaatatc ctcgctccgg gtatcgaccg gcgggagaac tgggtgttga
     3721 cgcggagttt cacgctgggc gctaacttag gattttaatt ttttatacta atacatgcat
     3781 aatatgaaaa ttagtttaat catccgactg tccgtcctcg tcatgctgat cacggtgggc
     3841 tcctgtacca agctggatga gacaaaatat ctatacgata cggtgaatgc cgatggattt
     3901 tatcaaaccg acgccgaact gtcttccgcg gtgggggccg cctatgccaa cctgaccagt
     3961 gtggcgggta acaaccatta tctca
//
LOCUS       DATGFG010000978         7103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_396855, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000978 DATGFG010000000
VERSION     DATGFG010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396855"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VKQ52_18460"
     CDS             complement(<1..558)
                     /locus_tag="VKQ52_18460"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HLZ89249.1"
                     /translation="MFNLILFGPPGSGKGTQSERLISKYGLKHLSTGDLLRSEISGQT
                     PLGLAAKSYMDKGQLVPDEVVIEMISSALDNNPLAHGFLFDGFPRTTAQAEALDKLLN
                     QKGTSIVVVLALQVGENELIKRLLNRGLTSGRPDDVNEEIIRNRIAEYEKKTAAVADH
                     YRKYDKVVYIQGEGDVDEIFVSLSKE"
     gene            623..1072
                     /gene="msrB"
                     /locus_tag="VKQ52_18465"
     CDS             623..1072
                     /gene="msrB"
                     /locus_tag="VKQ52_18465"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLZ89250.1"
                     /translation="MDTKDPAGKENNPVYSHTDASAVNLTDAQWKTILPGDVYAVARD
                     KGTEQPWTSPFEGSMEIGTYYCAACGNPLFRSDTKFESGCGWPSFYEPVGRGSILYAA
                     DHSHGMDRTEVLCGRCKSHLGHVFDDGPPPRGLRYCINGVVLDFEKK"
     gene            1129..1201
                     /locus_tag="VKQ52_18470"
     tRNA            1129..1201
                     /locus_tag="VKQ52_18470"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1162..1164,aa:Thr,seq:cgt)
     gene            1206..2468
                     /locus_tag="VKQ52_18475"
     CDS             1206..2468
                     /locus_tag="VKQ52_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ89251.1"
                     /translation="MALKLSYLAETLIGSEIVKLGGEIREKIRKGEKIYNFTVGDFDP
                     GIFPIPRELEELIIDAYRRHFTNYPAAEGNLDLRESISAFLEEREALTYGTNEILVAS
                     GGRPLIYSLFRTIVDKGDKVIYAVPSWNNNHYTHLNQGEHVVIEATAENNFMPTAEAL
                     RPFIREAALVCLCSPQNPTGTTISKKDLSAICELVLEENQRRGAGEKKLYLLYDQMYW
                     QLTYGEIRHYNPVSLYPAMRDYTIFIDAISKAFAATGVRVGWAFGPAEIISKMNAIMS
                     HIGSWAPMAEQKATAVFLTKKEAIDTYLTGFRAAVSDRLRQIHDGFVLLKKEGFAVDV
                     LAPEAAIYLTIKIDLKGKKTDAGVVLNDQMAVTSYLLNEAGLAVVPFSAFGAAKSSPW
                     YRLSVGTCKLQDIPEMIGKLREAMRRLG"
     gene            complement(2486..2734)
                     /locus_tag="VKQ52_18480"
     CDS             complement(2486..2734)
                     /locus_tag="VKQ52_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89252.1"
                     /translation="MKTCNRDLLVLVKDEHMNEKSMERELEQLNDLLFHFETIETFCT
                     AHEVFDINRHKTLHKRDSIQRIVRQKELKPFFFICNKN"
     gene            3224..4075
                     /locus_tag="VKQ52_18485"
     CDS             3224..4075
                     /locus_tag="VKQ52_18485"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ89253.1"
                     /translation="MIRIKQERAWLRGFAAREELDLVISDNRYGLIVPGVFCVFLTHQ
                     LLIRTPFGRPADLLLQRMNYRLIRRFSRCWVPDVAGAGALAGVLSNPVRMPPIATRYI
                     GWLSRMGSGVGPVAEEHDLLVLLSGPEPQRTMLERLVLKQAADLPCRLVLVRGLPGGG
                     PVLKGVPATVTVHDHLPAAALERVMAGARLVVARSGYSTVMDLARMGKRVALVPTPGQ
                     TEQEYLGRYLAEKGWAVCVPQRDLVLRDVLRAPVPGPGWPPADGGQRLREEVLSVLRQ
                     SRPSALG"
     gene            complement(4048..4548)
                     /locus_tag="VKQ52_18490"
     CDS             complement(4048..4548)
                     /locus_tag="VKQ52_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLZ89254.1"
                     /translation="MKTLLIIRHAKSSWDDASVNDIDRPLNDRGKRDAPAMAHRLIKA
                     GITIDSFVSSPAKRARQTAEAFMHAFGKKEKHLRLITDLYHAAPSTFEKVVDGLDDED
                     DTVAIFSHNPGITSFVNTLTSIRLDNMPTCAIFAAKCDTRHWKDFRSAGPQFWFLDYP
                     KAEGRD"
     gene            complement(4570..5211)
                     /locus_tag="VKQ52_18495"
     CDS             complement(4570..5211)
                     /locus_tag="VKQ52_18495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ89255.1"
                     /translation="MKKVSIMIVDDHTLIRETWSFLLGKNENFDVVAECGDGERAIEL
                     ARDKRPDVVLLDINMAPMSGFDVLKMIRKYSPGSKIIGVSMHSQPAYAKKMLRLGAKG
                     YVTKNSPRQEMLEAIAEVSNNHIYICQEVKNILSDQLLNNDQVNPDINNLSDREMQIV
                     RALKEGLSSKEIASELSISLKTVEVHRHNILKKLKLKNTVSLINFINSQAFDI"
     gene            complement(5346..>7103)
                     /locus_tag="VKQ52_18500"
     CDS             complement(5346..>7103)
                     /locus_tag="VKQ52_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89256.1"
                     /translation="LYGLNKLYLLMEKQHNRGQDGAGVASVKLNIEPGHPFLHRIRSA
                     NHQPIADIFSKIMGEVKDLEQYQPDITQHPGLMKGHISHLGELLLGHLRYGTQGRNLV
                     EFCHPFIKRNTIPAHNLALAGNFNLVNTDELFDLIGIDPGEFQKQSDLAAMMEVIHHF
                     LVRADEASPGSLDLAGLLKKAVPLFDGGFTVGGLIGNGDSFVFRDAHGIRPAYYYVSE
                     DVVVAASERAAIRTVFNVGENEVMELMPGKGLIVKADGAVSVETILEPKERRACSFER
                     IYFSRGSDEKIYRERIALGYNLSEQILTAINHDLKNTIFSFIPNTAEVAFYGLWKGMD
                     DYLEKIKVQRILSWGKDISEEKLREMLSRKIRVEKVAIKDVKMRTFITADSSRNEMVQ
                     HVYDVTYGTVQRDTDTLVVIDDSIVRGTTLKESIIRMLARLHPKKIIVVSSAPQIRYP
                     DCYGIDMSKLGDFIAFRAAVELMKDRGREDCLQELYDRCKELARTGQLHTENVVQRVY
                     KPFTAEEIAARIAQLITPPEINTPVQVIFQTIESLHRSCPTNTGDWYFTGNYPTPGGN
                     RVVNTAFMNYMEGKNIRGY"
BASE COUNT         1466 a         1702 c         2152 g         1783 t
ORIGIN      
        1 ttccttactc agggagacga agatctcgtc cacgtcgcct tcgccctgga tatacactac
       61 cttgtcgtat ttccggtaat ggtcggctac ggcggccgtc tttttctcgt attcggcgat
      121 acggttgcgg atgatctctt cgttgacgtc atcgggccgg ccggaggtaa ggccgcggtt
      181 gagcagtcgt tttatgagtt cgttctcgcc gacctggagg gccaggacga cgacgatgga
      241 ggtacccttc tggttgagca gtttgtcaag ggcttcggcc tgggcggtgg tgcgggggaa
      301 gccgtcgaaa aggaagccgt gcgccagggg attattgtcg agggcggagc tgatcatctc
      361 gatgaccacc tcgtccggaa cgagttgtcc tttatccata tagctctttg ctgccagccc
      421 gaggggcgtt tgaccggaga tctcgcttcg aagcagatcc ccggtgctga ggtgcttcag
      481 gccatatttg gagatcaatc gttcagactg tgtacccttc ccgctaccgg gagggccgaa
      541 taaaattaaa ttgaacataa tacgtactac ttacccttgt gagttgacaa atataatata
      601 caagcctcaa aaatcaagca atatggatac aaaagaccct gcgggtaagg aaaacaaccc
      661 tgtttattcg cataccgacg cttcggcggt aaatctgact gatgcccaat ggaaaacgat
      721 cctgcccggg gatgtctatg cggttgcgcg ggacaagggt acagagcagc cctggaccag
      781 tcctttcgag ggttcgatgg agattgggac atattactgc gccgcctgtg gcaatccatt
      841 gttccgcagc gataccaagt ttgaaagcgg ttgtggctgg ccgagtttct atgagcctgt
      901 cggcagggga agcatccttt atgctgccga ccattcccat ggcatggacc ggacggaggt
      961 gttgtgcggg cgctgcaagt cgcacctggg gcatgttttt gacgatgggc cgccgcccag
     1021 ggggctgcgg tattgcatca atggggtggt tttggatttc gaaaagaagt aagatttacc
     1081 tatttttgca ccccgatcgc ccaggggaac catcctggtg gcgcatcagc caacttagct
     1141 cagctggtag agcatttcat tcgtaatgaa agggtcgtcg gttcgattcc gatagttggc
     1201 tcatcatggc tttaaagctg tcttatcttg ccgaaaccct tatcgggtcg gagatcgtca
     1261 aattaggtgg cgagatccgt gaaaagatcc gaaagggaga aaagatctac aattttaccg
     1321 taggcgattt cgatccgggt atttttccca ttccccggga gctggaggag ctgatcatcg
     1381 acgcttaccg ccggcatttc accaactatc ccgcagcaga aggcaatctc gatctccgtg
     1441 agtcgatctc cgcattcctg gaagagcggg aggcgttgac ctacggcacc aatgagatcc
     1501 tggtggccag tggcggccgg ccgcttatct attcgctttt ccggacgatc gtcgacaagg
     1561 gggataaggt gatctatgct gttccttcgt ggaataacaa tcattatacg catctgaacc
     1621 agggtgagca tgtcgttatc gaggcgacgg cggagaacaa tttcatgccg acggcggaag
     1681 cgctgcgacc gttcatccgg gaggcggcgc tggtctgtct ttgcagcccg cagaatccca
     1741 cgggtactac gatctccaag aaagacctct ccgcgatctg tgaattggtg ctggaggaga
     1801 atcagcggcg gggcgcgggg gagaagaagt tgtacctgtt gtatgatcag atgtactggc
     1861 agctgaccta tggggagatc aggcattaca atcccgtttc cctgtatcct gccatgcggg
     1921 actatacgat ctttatcgat gccatcagca aggcttttgc cgcgacgggc gtgcgggtcg
     1981 gctgggcatt cggtccggcg gagatcatca gcaagatgaa tgcgatcatg agccatatcg
     2041 ggtcctgggc gccgatggcg gagcagaagg caacggctgt ctttcttacg aagaaagagg
     2101 cgatcgatac gtatctgacc gggtttcgcg cggcggtatc ggaccggctg cggcagatcc
     2161 acgatgggtt tgtattgttg aagaaggaag ggttcgctgt ggatgtgctt gcgccggagg
     2221 cggccatcta cctgacgata aagatcgatc tgaaagggaa aaagacggat gcgggtgtcg
     2281 tgctgaatga ccagatggct gtcacctctt atcttctcaa cgaggcaggt cttgccgtgg
     2341 tgccgttctc ggcatttggc gcggcgaaga gctctccctg gtaccggctg agcgtgggaa
     2401 cgtgtaagtt gcaagatatt cctgaaatga tcgggaagct gcgggaagcg atgagaaggc
     2461 tcggctgatc cggccggctg gggggctagt tcttattaca gataaagaag aagggtttca
     2521 attctttttg gcgaacgata cgttggatgc tgtcgcgttt gtgcagggtc ttatgcctgt
     2581 tgatatcgaa gacttcgtga gcagtacaaa acgtttcgat ggtttcgaaa tggaacaaca
     2641 ggtcatttaa ttgttccaac tcacgctcca tcgatttttc attcatgtgt tcatctttca
     2701 ccagaacaag caaatccctg ttacaagttt tcattctatt agattttaat aaccagacat
     2761 acccttgtta attccgcagc cacatttttt actgctgccg ccaacccccc ggcccgaact
     2821 gcagccgaag aatacaatgc tactaaataa taataacaga atcaatatct gttttgtggt
     2881 tttcataatc tttaatccta ctaatttaac tattttgtat cgaaaatagt attctccctt
     2941 gccaggtcct gtaaaaatta acaatcctac ttacgaatct ctaaagattc tcgtggctcc
     3001 gctggattgg ggactagggc acgcaaccag atgtatccca ataattaagg aattactaaa
     3061 tcaaagatgt gaggtgatcg tcgccgctac cggcgcacaa cgagcgctcc tgcaggagga
     3121 atttgcgtct ttgaccttcg tagaattacc cgggtatcgc gtaaaatacg gcaaaaatcg
     3181 agccattaca atgctgcggg taatttgttc gatcccgaaa atcttgatcc gtatcaagca
     3241 ggaaagggcc tggctgcggg gttttgcggc gcgggaggag cttgacctgg tgatctcgga
     3301 taaccgttac gggcttatcg tccccggggt cttttgtgtt tttcttacgc atcaactgtt
     3361 gatccggacg ccttttggcc ggccggcgga ccttttgctg cagcggatga actacaggct
     3421 tatccggcgt ttctcgcggt gctgggtacc ggatgtggcg ggagcggggg cgttggcggg
     3481 tgtgttgtcg aaccctgtgc ggatgccgcc tattgctacg cgttatatcg ggtggttgtc
     3541 gcggatgggt agcggtgtgg gtcctgtcgc ggaagagcat gatctgcttg tgcttttgtc
     3601 gggtccggag ccgcagcgca cgatgctgga gcggctggtt ctgaagcagg cggcggatct
     3661 tccctgccgg cttgtgctgg tgcgggggtt acccgggggt ggaccggtgc tgaagggtgt
     3721 cccggcgacg gtgacggtgc atgatcatct gcccgcggcc gcgctggagc gggtgatggc
     3781 gggggccagg ctggtggttg cccgttcggg ttatagtacg gtgatggacc tggcgaggat
     3841 gggtaaacgg gttgcgctgg tgcctacgcc cgggcagacc gagcaggagt atcttggccg
     3901 gtacctggcg gagaagggct gggcggtctg tgtgccgcaa cgggacctgg tgctgaggga
     3961 tgtcctgcgg gcgccggtac cggggccggg ctggcctccg gccgatgggg gtcaacggtt
     4021 acgggaagag gtattgtcgg tgttgcgtca gtcgcggcct tcggccttgg ggtagtctaa
     4081 aaaccagaat tgcggaccgg ccgaccgaaa atccttccag tgccgggtat cgcattttgc
     4141 ggcgaaaatg gcgcaggtgg gcatattatc gaggcggatg gaggtcaggg tgttgacgaa
     4201 ggaggtgatc ccggggttgt gggagaagat cgcgacggtg tcgtcctcat cgtcgagtcc
     4261 gtcgaccact ttttcgaagg tggatggggc ggcgtgatag aggtcggtga tgagccggag
     4321 atgtttttct tttttgccga aggcgtgcat aaaggcttcc gccgtctgcc gggctcgttt
     4381 ggcggggctg gaaacaaagc tgtcgatggt gatgccggcc tttatgagcc ggtgggccat
     4441 ggcgggtgcg tcccgcttcc cccggtcgtt gagggggcga tcgatgtcgt ttacggaggc
     4501 atcgtcccaa ctgctttttg catgccggat gatcagcagg gtcttcatcc ggctaaacta
     4561 taaaaaatat caaatatcga acgcctgtga gttgataaaa ttgataaggg agacggtatt
     4621 cttgagtttt agctttttga ggatgttgtg ccggtgaacc tccaccgtct tcagggagat
     4681 gctgagttcg gaggcgatct ccttggagga gaggccttcc ttcagggccc ggacgatctg
     4741 catctcgcgg tcggagaggt tattgatatc cgggttgacc tggtcgttat tgagcaactg
     4801 gtccgagagg atgttcttta cttcctggca gatatagata tggttgttgc ttacctcggc
     4861 aatggcttcg agcatttcct ggcggggaga gttcttggtg acgtagccct tggcgccgag
     4921 ccggagcatt tttttggcgt aggcgggttg ggagtgcatg gataccccga tgatcttgga
     4981 gccgggtgag tatttgcgga tcatcttgag aacgtcgaac ccgctcatcg gagccatatt
     5041 gatatcgagg agcacgacat cggggcgttt gtcccgggcc agttcgatcg cccgttcccc
     5101 atccccacat tctgccacga catcaaaatt ctcatttttt cccagtagaa aactccatgt
     5161 ttcacggatt aaggtatgat catcgacgat cataatagaa actttcttca taacgcttgt
     5221 tttttctaag ccagaaaaca gtcaaacatt actaatgcag tgcattaacc agacagaagt
     5281 tactttaagg ggatgggtac aaatagctat ataatattaa ggattttctc tgataacttc
     5341 aaaatttaat accctcttat gttctttcct tccatataat tcataaaggc cgtattgacg
     5401 accctgttgc cgcccggtgt cgggtagtta ccggtgaagt accagtcacc agtattggta
     5461 ggacaggagc gatggaggga ttcgatggtc tggaagatga cctggacggg ggtattgatc
     5521 tcgggagggg tgatcagctg ggcgatcctt gcggcaatct cttcggcggt gaagggtttg
     5581 tagacgcgtt ggacgacgtt ttccgtgtga agctggccgg tacgggccag ttctttacag
     5641 cgatcgtaca gttcctggag gcagtcttcc ctgccgcgat ccttcatcag ctccaccgct
     5701 gcgcggaagg cgatgaagtc gcccagttta ctcatatcga tgccatagca atcggggtaa
     5761 cggatctggg gggcggagga caccacgatg atcttctttg ggtggagacg ggcgagcatc
     5821 cggatgatgc tttcctttag ggtcgtaccc cgaacgatcg agtcatcgat cacgacgagg
     5881 gtatcggtgt ctctttgcac tgttccatag gtgacgtcat agacgtgctg gaccatctcg
     5941 ttgcggctgg aatccgccgt tatgaaggtc ctcatcttca cgtcctttat ggctactttt
     6001 tccacccgta tcttgcggct gagcatttcc cgcagcttct cctcggaaat atccttcccc
     6061 caggagagga tgcgctgaac cttgatcttt tccaggtagt cgtccattcc tttccagagt
     6121 ccgtaaaagg ccacctcggc cgtattgggg ataaaggaaa agatagtatt cttgaggtcg
     6181 tggttgatgg cggtgaggat ctgttcgctc aggttatagc caagggcgat gcgttcgcgg
     6241 tagatcttct cgtcgctgcc acgggaaaaa tagatgcgtt cgaagctgca ggcccgtctt
     6301 tcttttggtt ccaggatggt ttccacgctg acggcgccat cggcctttac gatcaggcct
     6361 ttgccgggca tcagctccat gacctcgttc tcgcccacgt tgaatacggt acggatggcc
     6421 gcgcgttcgg aggcggctac gaccacatcc tcgctgacat agtaatatgc cgggcggatg
     6481 ccatgggcgt cgcggaagac gaaactgtcg ccgttgccga taaggccgcc gacggtaaag
     6541 ccgccgtcga agagtggaac cgcttttttc aggagaccgg ccaggtcgag gctgcccggg
     6601 gaggcttcgt cggcccggac gaggaaatga tggatcactt ccatcatggc ggccagatcg
     6661 ctttgtttct ggaattctcc cggatcgatc ccgatgaggt cgaacagttc gtcggtattg
     6721 accaaattaa aattccccgc cagggcaagg ttgtgggcgg ggatcgtatt cctttttatg
     6781 aaggggtggc agaactcgac caaatttcgt ccctgggtgc catagcggag atggcccagg
     6841 agcagctcgc caagatgcga gatatgtcct ttcataagcc ccgggtgctg ggtaatatcg
     6901 ggttggtatt gttcgaggtc tttgacctcg cccattattt tggagaagat gtcggcgatg
     6961 ggctggtggt tggcgcttcg gatgcgatgc aggaaggggt ggcccggttc gatgttcagt
     7021 ttgacggagg cgactcccgc gccatcctgt ccccggttat gctgtttttc catcaggagg
     7081 tacagcttgt tcaagccgta cag
//
LOCUS       DATGFG010000979         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_784925, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000979 DATGFG010000000
VERSION     DATGFG010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784925"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VKQ52_18505"
     CDS             complement(<1..560)
                     /locus_tag="VKQ52_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89257.1"
                     /translation="MLTFLFFNWVGYWLFISWFESRETARWEVRLDNDQYDAGQLTLF
                     KVSAAEIPYSNASARFERADGELEVGNIHYRYVRKRLYNDSLEFLCIPDGEAGHLRQA
                     KSAIIHLVTGLPGDNEHGKSLPTGKVSPDQLKAFYEDHPAFFICHFPARREKEFSGQS
                     PTPCPGYAREDRQPPRQPARPGLIRP"
     gene            618..1799
                     /locus_tag="VKQ52_18510"
     CDS             618..1799
                     /locus_tag="VKQ52_18510"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HLZ89258.1"
                     /translation="MDTGNKNILAVNGGSSSIKFSLYTCDAPPAKWLSGKITRIGLKD
                     TELTFTNNRNNKKERLPVQAAGLPEAAGFLSGWLQQQEGATPLAAVGHRVVHGLQHRQ
                     AAVIDEALLDDLQRISGYDPDHLPGEIELIRLFRSHYPGTPQIACFDTAFHSTLPRIA
                     RLLPIPRRYEAAGLQRYGFHGLSYAYILEELTRIAGPAAARGSVILAHLGNGASMAAV
                     KDGSSVDTTMGFTPVGGIVMGTRPGDVDPGVWWWLLDRERLSAEQFSHLINNECGLLG
                     VSETSPDMQDLLEKESSDTRAAEAVGLFCYQVKKTIGAYSAVLGGLDSLVFTGGIGER
                     SAAIRSRICSGLGYLGIELNQRANAHDELFLATEEARVGVYAIPTDEEWMIARTVKHF
                     I"
     gene            1796..>4042
                     /locus_tag="VKQ52_18515"
     CDS             1796..>4042
                     /locus_tag="VKQ52_18515"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HLZ89259.1"
                     /translation="MTTSTMIQPLETLAPETLRRMDAYWRAANYLSVGQIYLRDNPLL
                     RRPLAPEHIKHLLLGHWGTTPGQNFIYVHLNRLIRQYDLDMIYIAGPGHGGPALVANT
                     YLEGTYTEVYPNISRDEEGLQRLFKQFSFPGGIPSHVSPECPGSIHEGGELGYSLSHA
                     FGAVFDNPGLTVACVIGDGEAETGALATAWHSNKFLNPVTDGTVLPILHLNGYKIANP
                     TILARIPKVELGQLLTGYGWEPYFVEGDDPEVMHRVMAATLDTIIENIRIIREMAAHN
                     KDQVERPRWPMLVLRTPKGWTGPKTIDGLPVEGSFRSHQVPISDPATHPAHLRQLEDW
                     LKSYRPEELFDEKGALHPELAGLAPEGSRRMGANPHANGGLLLRDLRMPDFRQYAVQL
                     DEHGSVKASDTTILGGFLADVIRYNAEERNFRIFGPDETLSNKLDAVFKATNRQWGAE
                     MIEQDQYLARDGRVMEVLSEHQCEGWLEGYLLTGRHGLFNSYEAFIHIVDSMFNQHAK
                     WLKVTRELPWRRKIASLNYLLASHVWRQDHNGFTHQDPGFIDHVINKKAEIVRVYLPP
                     DANCLLSTMDHCLRSRHYVNVVVAGKHPAPQWLTMEEAVLHCTKGVGIWGWASNDQDT
                     EPDVVMAAAGDVPTLEILAAISILRQKLPELRIRMVNVVDLMKLQPQSEHPHGLSDFD
                     FDLLFTRNKPVIFAYHGYPWLIHRLTYRRTNHGNIHVRGYKEEGTISTPFDMTVLNDM
                     DRFHLVMDV"
BASE COUNT          857 a         1111 c         1246 g          828 t
ORIGIN      
        1 aaagggcgga taaggcctgg ccttgccggc tgtcgtggcg gctgccggtc ctcgcgggca
       61 taacccggac atggggtagg tgactgcccg gagaactctt tttccctgcg ggccggaaaa
      121 tggcagataa agaaagcagg atggtcctca taaaaggctt tgagctggtc gggtgaaacc
      181 tttccagtgg ggagggattt gccgtgttcg ttatcgcccg gaagcccggt cacgaggtgg
      241 atgatcgcgc tctttgcctg gcgcaggtgg ccggcctcgc cgtcggggat acaaagaaat
      301 tcaagcgagt cgttgtacaa ccttttccga acatatctgt aatggatgtt gccgacttcc
      361 agctcaccgt cggcccgctc gaacctggca gaagcattgg agtagggaat ttcggcggcg
      421 gatactttga aaagggtcaa ttggccggcg tcatactggt cattgtcgag ccgcacttcc
      481 caacgggccg tttcccggct ttcgaaccag gagatgaaaa gccagtaacc tacccaattg
      541 aaaaatagaa atgttaacaa taatatggtc gcaatctttc ttaacaatgg tacaaggtag
      601 ctaactttca gtaaataatg gatacgggaa ataaaaatat tcttgctgtc aacggcggat
      661 catccagtat caaattttcc ctgtatacct gcgatgcgcc tcccgcgaaa tggctttccg
      721 gcaagatcac ccgcatcggg ctaaaggata cggagctaac gtttaccaat aatcgaaata
      781 ataaaaagga gagattgccg gtgcaggctg ccgggcttcc cgaggccgcc ggctttctgt
      841 ccggctggct gcagcagcag gagggggcga cgccgctggc ggcggttggc caccgggtag
      901 tgcatggtct tcagcaccgg caggcggcgg tgatcgacga ggcgctgctc gacgacctgc
      961 aacggatcag cggctatgat cccgatcatc tgcccggcga gatcgagctt atccggctct
     1021 ttcgcagcca ttatccgggg acgccgcaga tcgcctgttt cgacacggcc tttcacagca
     1081 cgctgccgcg catagccaga ctgctgccga tcccccggcg atatgaggcg gcggggttgc
     1141 aacgatacgg ctttcatggg ttgtcctacg catatatcct cgaagagctg acccggatcg
     1201 ccggaccggc tgcggcccgg ggctcggtga tcctggcgca cctgggaaat ggggccagca
     1261 tggcggcggt gaaggatggg agcagcgtgg atacgacgat gggctttacg ccggtgggcg
     1321 gtatcgtcat gggcacccgg ccgggagatg tagatcccgg cgtgtggtgg tggctgctgg
     1381 acagggagcg gctcagcgcg gagcaattca gccatcttat caataacgaa tgcgggctgt
     1441 tgggggtgtc ggagacaagc ccggacatgc aggacctgct ggagaaggaa agcagcgata
     1501 cccgggccgc ggaggcggtg gggctttttt gttaccaggt gaagaagaca atcggcgcct
     1561 attccgccgt attgggcggt ctcgactccc tggtattcac ggggggcatc ggtgaacgat
     1621 ccgcggcgat ccggagccgg atctgttcgg gtttgggtta tcttggtatc gaactcaacc
     1681 agcgggccaa cgcccatgat gaactcttcc ttgccacgga ggaggcccgg gtgggggtat
     1741 atgccattcc cacggatgag gaatggatga tcgcaagaac cgttaaacac ttcatatgac
     1801 aacatcaacc atgatccaac cgctggaaac cctggcgccg gagacattgc gccgcatgga
     1861 tgcctattgg cgggccgcga actacctctc ggtggggcag atctacctgc gggataatcc
     1921 cctgttgcgc cggccgctgg ccccggagca tatcaagcat ctcctgctgg gacattgggg
     1981 tactacccca gggcagaatt tcatctatgt gcatctcaac aggcttatcc ggcaatacga
     2041 tctggatatg atctatatcg ccggaccggg gcatggtgga cccgcgctcg tggccaatac
     2101 ctacctggaa gggacttata cggaagtgta tcccaatatc agccgggacg aggagggctt
     2161 gcagcgtttg ttcaaacagt tctcctttcc cggcgggatc cccagccatg tatcacccga
     2221 atgtccgggg tcgatccatg aaggggggga attagggtat tccctcagcc atgcctttgg
     2281 cgctgtcttt gacaacccgg ggcttacggt tgcctgtgtc atcggggatg gggaagcgga
     2341 gacgggtgcg ctggcgacgg cctggcattc caacaaattc ctgaatcccg ttaccgatgg
     2401 aacggtatta cccatcctgc atctcaacgg ctataagatc gccaatccca cgatcctggc
     2461 gcggataccg aaagtggagc tgggacagtt gctcaccggt tatggctggg agccctattt
     2521 cgtggaaggg gatgatccgg aggtgatgca ccgggtcatg gcggcaaccc tcgataccat
     2581 cattgagaat atccgcatca tccgggagat ggcggcgcat aataaggacc aggtggaacg
     2641 accccggtgg ccgatgctgg tcctgcggac gccaaaaggg tggacggggc caaagacgat
     2701 cgacggcctt cccgtggagg gttcattccg tagccaccag gtgccgatct ccgatcccgc
     2761 tacccatccc gcgcacctgc ggcagctgga ggactggttg aagagctacc gcccggagga
     2821 attatttgat gaaaagggcg cgctgcatcc ggaactggcc gggttggcgc cggagggatc
     2881 gaggcggatg ggcgcaaatc ctcatgccaa cggcggcctg ctgctcaggg atctccggat
     2941 gcccgatttc cggcagtatg ccgtacaact ggatgagcat gggtctgtaa aggcttctga
     3001 taccacgatc cttggcgggt tcctggcgga cgtcatccgc tacaacgccg aagagcggaa
     3061 cttccgcatc ttcggacccg acgagaccct gtcgaacaaa ctcgacgccg tcttcaaggc
     3121 caccaaccgg cagtgggggg cggagatgat cgaacaggac cagtatctcg cacgcgacgg
     3181 ccgggtaatg gaagtgttga gcgagcacca gtgtgaaggc tggctggaag gctatctgct
     3241 caccgggcgg catggattgt tcaacagcta tgaggccttt atccatatcg tcgacagtat
     3301 gttcaaccag cacgccaaat ggctgaaggt gacgcgggag ctgccctggc gccggaagat
     3361 cgcttccctc aactacctgc tggcttccca tgtctggcgc caggaccata acggcttcac
     3421 ccaccaggac cccggattta tcgatcatgt catcaataag aaggcggaga ttgtgcgggt
     3481 ctacctgccg cccgatgcca attgtctcct ctcgacgatg gatcattgtc tgcgcagccg
     3541 gcattatgtc aatgtcgtcg ttgccggcaa acatcccgca ccgcaatggc tgacgatgga
     3601 agaggcggtc ctgcattgca cgaagggcgt cggcatctgg ggctgggcca gcaatgacca
     3661 ggacacggag cccgatgtcg tgatggctgc cgcgggggat gttcctacgc ttgaaattct
     3721 ggccgccatt tccattctcc ggcagaagct gccggagctg cggatccgga tggtcaatgt
     3781 ggtcgatctg atgaagctgc agccgcaaag cgaacacccg catgggctga gcgatttcga
     3841 tttcgatctg ctgttcaccc gtaacaagcc ggtgatcttt gcctaccatg ggtacccctg
     3901 gcttattcac cggctcacct accgccgcac gaaccatggc aatatccatg tgcggggata
     3961 taaggaggaa gggaccatct cgacaccttt cgatatgacc gtcctcaacg atatggatcg
     4021 ttttcacctc gtgatggatg tc
//
LOCUS       DATGFG010000980         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1424468, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000980 DATGFG010000000
VERSION     DATGFG010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424468"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VKQ52_18520"
     CDS             <1..917
                     /locus_tag="VKQ52_18520"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ89260.1"
                     /translation="DSTKIEVVAGVSKTFYGLLSTLQQISVLKEDTARLAKNVSDAYH
                     QYVGGIVDETDYDEAVITLNNSRFQLKQATENVSPQYALLKQVMGFPPRSQFNVIYDT
                     LQMMTDIAFDTTQELQFEKRIEFQVIQTQKQLQLANVNYYRKAWLPTVGGFFNYDYEF
                     QNNSFGNLFSKAYPYSYMGLTVTMPVFTGFSRLNNIKRARLQGDLLDWSEVSLKSQIW
                     SEYTTALANYKSNLYNLTVSKDNVNLAKRTYDIVALQYQQGVVPYLNVITAESNLITS
                     EISYQNALFQVLSSKIDLEKSMGIIVVH"
     gene            957..2189
                     /locus_tag="VKQ52_18525"
     CDS             957..2189
                     /locus_tag="VKQ52_18525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ89261.1"
                     /translation="MNRLASMLIFLSGLALFSCQQKPQAPANPATPVNLATVKSQPVY
                     YFDDYPATTQALSQVDLHAQVTGYVTAILFTEGTHVHKGQRLYEIDERLFKASYDQAV
                     ANVKVAQGNQEQAQQDADRYQYLIQHNAVAKQTLDHAVIALQNAKNETAAAEQALKTA
                     ATNLTYSVISAPFDGTIGFSQVKLGNLVTASSTILNTVSTDNPMAVDFLINEAQLNHY
                     QELQRDKQHKLDSLFTIVLPDHSMYPHTGKISVIDRAVDPQTGTIRVRLEFNNPKLDL
                     RAGMSCVVRVHNLEQAPQVVIPARAVIEQMGEYFVYIAKDTVIHNGADSTAKDGAGKA
                     AADPAGKPAGDPANAPQLRAVQVKVETGQTIGSNIVIRSGLHDGDRLVVDGVQALHDG
                     SPITTANTMGPGQAGRRR"
     gene            2199..>3310
                     /locus_tag="VKQ52_18530"
     CDS             2199..>3310
                     /locus_tag="VKQ52_18530"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ89262.1"
                     /translation="MIANTFIKRPVTAIVISIVLVLTGAICIVILPVDQYPDITPPIV
                     QVNGQFTGADAQTVEQTVATPIEQQVNGTPGMEYMQSNSTNNGLMSINVTFNIGTNID
                     VAALDVQNRVSIATPLLPAVASRLGITVRAVNPSMLMMVAIYAPKGTHNITFLDNYAN
                     IFVQDALLRVPGVGTIQRFTDDFSMRVWMNPQKMAAYSLTPQDVINALNAQNVQVAAG
                     SAGVPPQNKSQTFEIGILVNGRLSKPKEFENIIVKTLPATGELVYLKDVARVELGKFT
                     FASNSFVDGKRASYLQIYQAPGSNALRTAEGIYTELAQLKQFFPADVEYSVPFESVTV
                     VKVSMADVVDTLLLTLGLVALVVYLFLQNFRSTLIPV"
BASE COUNT          781 a         1095 c          832 g          602 t
ORIGIN      
        1 ccgacagcac caaaatagag gtcgtggccg gggtaagcaa gaccttctac ggcctcctgt
       61 cgacgctgca acagatcagc gtcctgaaag aggataccgc ccggctggcc aagaacgtga
      121 gcgacgctta tcaccagtac gtcggcggca tcgtcgacga aacggactac gacgaagccg
      181 tcatcaccct gaataattcc agattccagc tgaaacaggc aacggagaac gtcagccctc
      241 aatacgccct gctgaaacag gtgatgggct tccccccgag gtcgcagttc aatgtcatct
      301 atgatacgct gcagatgatg accgatatcg cctttgacac cacccaggaa ctgcagttcg
      361 agaaacgcat cgaattccag gtcatacaga cccagaaaca actgcagctg gccaatgtca
      421 actactatcg caaggcatgg ctgccgacgg tgggcggttt cttcaactat gattacgaat
      481 tccagaacaa tagctttggc aacctctttt ccaaagccta cccctacagc tatatgggcc
      541 tcacggtgac catgcctgtc ttcaccgggt tcagccggct caataacatc aaacgggcgc
      601 gcctgcaggg cgacctgctc gactggagcg aagtgtccct gaagtcgcag atctggtcgg
      661 agtacaccac cgccctcgcc aattataaga gcaacctgta caacctgacc gtttcaaaag
      721 acaatgtcaa cctggcgaaa cggacctatg atatcgtagc gctgcaatac cagcagggcg
      781 tcgtccccta tctcaatgtg atcacggccg agtccaatct gatcacctcg gagatcagct
      841 accagaatgc cctgttccag gtgctgtcga gcaagatcga cctggaaaaa tccatgggca
      901 tcatcgtcgt ccattaaccc gaaccaatgt acatcattaa gaaaaatatc acttgtatga
      961 acagactggc atcgatgctt attttcctca gcggcctggc actcttctcc tgccagcaga
     1021 agccccaggc ccccgctaat cccgctaccc ccgtcaacct cgccacggtg aagtcgcaac
     1081 ccgtctacta cttcgacgac tatcccgcta ccacccaggc gctgagccag gtggacctgc
     1141 acgcccaggt caccggttat gtgacggcca ttctgttcac cgagggaacc catgtgcaca
     1201 agggccagcg attatacgag atagacgaac ggctgttcaa ggcctcctat gaccaggccg
     1261 tcgccaacgt aaaagtggcg cagggcaacc aggaacaggc ccagcaggat gccgatcgct
     1321 atcagtacct catccagcac aacgccgtag ccaaacaaac cctcgaccac gcggtcatcg
     1381 ccctccagaa tgcaaaaaat gaaacggccg ccgccgaaca ggccctgaag acggccgcca
     1441 ccaacctcac ctactcggtg atcagcgccc ccttcgacgg aaccatcggc ttcagccagg
     1501 tgaagctggg taacctcgtc acggcgagct ccaccatcct caacactgta tccaccgata
     1561 accccatggc agtggacttc ctcatcaacg aggcacagct caaccattac caggagctcc
     1621 agcgcgataa acagcataaa ctggattccc ttttcaccat cgtcctcccc gaccactcca
     1681 tgtatccgca taccggcaag atctcggtca tcgatcgggc cgtcgacccg caaacgggta
     1741 ctatccgtgt ccgtctggag ttcaacaacc ccaagctgga tctgcgtgcg gggatgtcct
     1801 gcgtggtgcg ggtgcataac ctcgaacagg cgccgcaggt cgtcatcccc gcccgggccg
     1861 tcatcgaaca gatgggcgaa tacttcgtct acatcgcaaa ggacaccgtc atacacaacg
     1921 gcgcggactc caccgccaaa gacggagccg gcaaggctgc cgccgatccc gcggggaaac
     1981 ccgccggcga cccggccaat gccccccaac tgcgggccgt ccaggtgaag gtcgagacgg
     2041 gtcagaccat cggctccaat atcgtcatcc ggtcgggact ccatgacgga gaccgcctcg
     2101 tcgttgacgg cgtgcaggcc ctgcacgacg gctcgccgat caccacggcc aacaccatgg
     2161 gacccggcca ggccggccgt cgccgctaaa ctactatcat gatcgccaat accttcataa
     2221 agagaccggt caccgccatc gtcatctcca tcgtgctggt cctcacgggt gccatctgca
     2281 tcgttatcct gcccgtcgac caatacccgg atatcactcc gcccatcgtc caggtgaacg
     2341 gccagttcac cggtgcggac gcgcagacgg tggaacaaac cgtcgccaca cccatcgaac
     2401 aacaggtgaa cggtaccccg ggaatggagt atatgcagag caacagcacc aacaacggcc
     2461 tgatgtcgat caacgtgacc tttaacatcg gcaccaatat cgatgtggcg gcgcttgacg
     2521 tgcagaatcg ggtcagtatc gcaactcccc tgctgcccgc ggtggcgagc cgcctcggga
     2581 tcacggtccg cgcggtaaac cccagcatgc tgatgatggt ggccatttat gccccgaagg
     2641 ggacgcataa catcaccttc ctggataact acgcgaatat cttcgtacag gacgcgctgc
     2701 tgcgcgttcc cggcgtcggc accatccagc gattcacaga cgacttctcg atgcgtgtct
     2761 ggatgaaccc gcagaagatg gcggcctaca gcctcacccc gcaggatgtg atcaacgccc
     2821 tcaacgccca gaacgtccag gtagcggcgg gctccgcggg cgtgccgccg cagaacaaat
     2881 cccagacctt cgagatcggc atcctggtca acggccgcct cagcaagcca aaagaattcg
     2941 agaacatcat cgtcaagacc cttcccgcca ccggcgaact ggtctatctc aaggatgtcg
     3001 cccgcgtgga attaggcaag ttcaccttcg ccagcaattc cttcgtcgac gggaaacgcg
     3061 cttcctatct ccagatctac caggcccccg gcagcaacgc gctccggact gccgaaggga
     3121 tctataccga actggcgcag ctgaagcagt tcttccccgc cgacgtcgaa tattccgttc
     3181 ccttcgagtc ggtgaccgtg gtgaaagtat cgatggccga cgttgtcgat accctcctgc
     3241 tgaccctggg ccttgtagca ctggtggtgt atctcttttt gcagaatttc cgctccacgc
     3301 tgatccccgt
//
LOCUS       DATGFG010000981         6106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_852805, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000981 DATGFG010000000
VERSION     DATGFG010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852805"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VKQ52_18535"
     CDS             <1..1221
                     /locus_tag="VKQ52_18535"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ89263.1"
                     /translation="YPAFILSGYQPVIVLRGLFKHSTQGIGLRKALIVGQFTASILLI
                     GGTIIVYQQVDYMRNQQLGIDIHQTLVLDGASTPGDSTYRVAYQSFRNELLQLNGVKN
                     VTASSNVMGQEILFGIGTARESAKGKEQYMMYYLAVDYDFMPAYGLRQLAGRNFSPNF
                     RLDKKAVFINEAAVRTLGFSSPEKALHEYIEGYSDSLQIIGVVSDYHHQGLQKAIDPM
                     VFILQPDVRDFYSLKLEATGGRLPQTVDAISAIWTRHFPNDPFNYFFLDDFFNRQYKA
                     DQEFGKVFGLFATLAILIACFGLLGLSAYNVVQRTKEIGIRKVLGATVRQLLYLLSRD
                     FVTLVLVAFVIAVPLTWWLMDHWLRDFAYRIHIGWWVFAVAGVLAVLIAVLTVVFQAW
                     RTATANPVRSLRSE"
     gene            complement(1232..1888)
                     /locus_tag="VKQ52_18540"
     CDS             complement(1232..1888)
                     /locus_tag="VKQ52_18540"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HLZ89264.1"
                     /translation="MVNKQKKTPPPVTHGPLVRLDSSFYDRPGVVPVAKELLGKVLVT
                     EFDGARTSGRIVEVEAYNGVVDRASHAWSGRRTQRTEVMFGPGGVAYVYLIYGIHHLF
                     NVVTNRREVPHAVLVRALEPLEGIPVMLQRTGKKKPDKTLTRGPGNLSKALGLHVTHT
                     GTSLVGDAIYIADDGWRPRRSEIVATPRIGVDYAGEDAALPYRFFIRGNPYVSGPKGG
                     "
     gene            1954..2715
                     /locus_tag="VKQ52_18545"
     CDS             1954..2715
                     /locus_tag="VKQ52_18545"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HLZ89265.1"
                     /translation="MLKEIIIAFQSYVKAHQFITKHKLWKWIIIPGVFYTILFCIGMY
                     FFWDSSGTAVAWLSRVIGIDHFLHHRQSAALSFLFLMGGIMVHLILLFFYFSLFKYLF
                     LIIGSPLFAYLSQQTEALITGKQFQFQPNHQLWLDLRRGIRVALRNSLWQTVYTISIL
                     ILSFIPLVGWITPVVSTFVECYYYGFSMCDYSCQRHNLSPTASIAYISQHKGLAIGNG
                     MVFYGMHFVPIIGWVLAPAYAVIAATISLYHQNLE"
     gene            2992..3981
                     /locus_tag="VKQ52_18550"
     CDS             2992..3981
                     /locus_tag="VKQ52_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89266.1"
                     /translation="MTSYDKILHLFEQSNELTVKEIVDKLGISKQMTHLAVNRLRQEN
                     RLEKLGSPPKTVYRYLSPDHQVEEPPADYDATDKDQQYLNANFTVVTDTGNLLDGIAA
                     FEYWCRQRKLPVAKTLKEYIDTKKKYAPYYDEEGMVNGLEKLKNTKGYDHIWLDDLCY
                     LDFYAIERFGKTRLGTILHYAKQGQNKFLMRLMMELIGPRLKTFLQAQGADAVGFIPP
                     TIRREVQLMKVIQAQLNIALPVVEIKKISGLIPVPQKSLSKLDERIRNAENTFVVTDR
                     RQFRHVVLIDDAVGSGSTLNQVAAKMKNKGIAQTITGVAIVGSFKGFDVITDI"
     gene            3992..4849
                     /locus_tag="VKQ52_18555"
     CDS             3992..4849
                     /locus_tag="VKQ52_18555"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLZ89267.1"
                     /translation="MTNTVPTNTIPAATNAATLYLIPCWLEEQIITPLPVYILDAVKQ
                     CTVFFVENERSARRYLKAIWKEMVIDDYEWYPIHKAEEEVKATFRQKLKEGRTIGLIS
                     EAGCPGIADPGQILTEAAHQAGATVRPLVGPSSILLALMASGLNGQQFRFTGYLPVDN
                     SGRAKALKDLETESQRLHCTMLFIETPYRNNQLLETLLATCRPTTRLCIAANLTAPTE
                     YIRTLPVAEWRKLPKPAAASNPGTPSTAAPGPTGPHSPAHSAPGNPTAHQATLPDLHK
                     QPAIFALLA"
     gene            4990..>6106
                     /locus_tag="VKQ52_18560"
     CDS             4990..>6106
                     /locus_tag="VKQ52_18560"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLZ89268.1"
                     /translation="MFNRQVIEQLEDWKHQSNRKPLILRGARQVGKTTAVNAFGRRFP
                     QYIYFNLEDAPDREIFAENADIHRLVQTIFLRKSKSLARRSETLIFIDEIQQFPQALN
                     MLRYFYEQYPEIAVVAAGSLLETIFAKGMSFPVGRVDFMVIRPVGFPEFLGAIDQEQV
                     LEQLLHVPMNDFAHSTLLQLFHTYTLVGGMPEIVDEYARTKDLTALSAIYERLITAYV
                     DDVEKYATNDHFVQVIRHCIRASFLDAGTRIKFQHFGRSNYNSREVGEALRTLEKTYL
                     LSLLYPTTDAVLPVRQDLKRSPRLHVLDTGMMNYFLGLRTEVLGTADLNSIYQGVIVE
                     HLVGQELLATQFNVLSRLDFWVREKAGSSAEVDYVYPF"
BASE COUNT         1398 a         2090 c         1360 g         1258 t
ORIGIN      
        1 tatcccgcgt ttattttatc gggctaccaa cccgtcatcg tcctccgggg gctcttcaaa
       61 cacagcacac agggcatagg actccgcaag gccctcatcg tcggccagtt caccgccagc
      121 atcttactca tcggcggcac catcatcgtc tatcaacagg tggactatat gcgcaatcag
      181 cagctgggca tcgatatcca ccagaccctc gtactcgacg gcgcatccac gccaggcgac
      241 tccacctacc gcgtcgctta tcagtcattc aggaatgagt tacttcaact caacggcgtg
      301 aagaacgtca ccgcctccag caacgtcatg ggccaggaga tcctgttcgg catcggcaca
      361 gcccgggaat ccgccaaggg aaaggaacaa tatatgatgt actatctggc cgtcgactat
      421 gatttcatgc cggcctatgg cctgcgtcaa ctggcaggtc gcaatttctc gccgaacttt
      481 cgccttgaca aaaaggctgt cttcatcaac gaggccgccg tacgcaccct gggcttctcc
      541 agccccgaaa aagcgctgca cgaatacatc gagggctata gcgattccct gcagatcatc
      601 ggcgtcgtct cggactatca ccaccagggc ctgcagaaag ccatcgaccc catggtcttc
      661 atcctgcagc ccgatgtccg cgacttctat tccctcaagc tcgaggccac cggcggccgc
      721 ctcccccaga ccgtcgacgc catcagtgcg atctggaccc gccatttccc caacgacccg
      781 ttcaactatt tcttcctcga tgatttcttc aatcgccaat acaaggccga ccaggaattc
      841 ggcaaggtct tcggcctctt cgccaccctg gccatcctca tcgcctgctt cggcctgctg
      901 ggcctgtcgg cctataacgt ggtacaacgc acgaaggaga tcggtattcg caaggtcctc
      961 ggcgccaccg tccggcaact gctctacctc ctctcccgcg acttcgtcac cctcgtgctc
     1021 gtggcttttg tcattgccgt gcccctcacc tggtggctca tggatcactg gctccgggac
     1081 ttcgcctatc gcatccacat cggctggtgg gtatttgccg tcgcaggcgt tctggctgtg
     1141 ctcatcgccg tcctcaccgt cgtcttccag gcctggcgaa ccgccaccgc caaccccgta
     1201 cggtccttgc gtagcgagtg agccgccggc cttacccgcc cttgggccca ctcacatacg
     1261 gattcccacg gataaaaaat cgatacggca gcgctgcatc ctcccccgca tagtcgaccc
     1321 cgatacgcgg cgtcgccacg atctcactcc gccgcggccg ccacccatca tcggcaatat
     1381 agatggcatc gcccaccagc gaagtccccg tatgcgtcac atgcaaccca agggccttcg
     1441 acagattgcc gggaccacgc gtaagcgtct tgtcaggttt cttcttgccg gtgcgttgca
     1501 gcatcaccgg tataccctcc agcggctcca gcgcccgaac aagcaccgca tgcggcacct
     1561 cccgccggtt ggtcacgaca ttgaacaggt gatgaatgcc atagatcagg tagacatagg
     1621 cgacgccgcc gggcccaaac atcacctccg tacgctgcgt ccgccgcccc gaccacgcat
     1681 gactcgcgcg atccaccacc ccgttatacg cctccacttc cacgatccgc cccgaggtcc
     1741 gcgctccatc gaactccgta accaacacct tccccaacaa ctccttcgcc acgggcacca
     1801 cacccggccg atcgtagaat gaactgtcca gccggacaag ggggccatgg gtcaccggcg
     1861 gcggagtctt cttttgttta ttgaccattt atacttaggt ttggcagatg gttatctgct
     1921 ctgcccgaac aaataaacaa aaagaaaagc accttgctta aagaaattat catagccttc
     1981 cagtcttatg taaaagctca ccagttcatc accaaacata agctgtggaa gtggatcatc
     2041 atcccgggtg tcttctatac gatcctgttc tgcatcggca tgtatttctt ttgggactcc
     2101 tccggcaccg ccgtagcctg gctcagccgc gtcatcggca tcgatcattt cctccatcac
     2161 agacaaagtg ccgcactcag cttcctcttc ctcatgggcg gcatcatggt ccatctcata
     2221 ctgctcttct tctacttctc gctattcaaa tatcttttcc tgatcatcgg ctcgccgctc
     2281 ttcgcctatc tcagccagca gaccgaagcc ctgatcaccg gcaaacaatt ccagtttcaa
     2341 cccaaccatc agctctggct cgatctgcgc cgggggatcc gcgtcgccct tcgcaactcc
     2401 ctctggcaga ccgtctacac catctcgatc ctcatcctct ccttcatccc cctcgtcggc
     2461 tggatcaccc ccgtcgtctc caccttcgtg gaatgttatt actacggatt ctccatgtgc
     2521 gactatagct gccagcggca taacctctcg cccaccgcca gcatcgctta catcagccag
     2581 cacaagggcc tcgccatcgg caacggcatg gtcttctatg gcatgcattt cgtccccatc
     2641 atcggttggg tcctcgcccc cgcctatgcc gtcatcgccg ccaccatcag cctctaccac
     2701 caaaatctcg aatagccgca cccggcccgc cccgcgccca ccagcacccg accatcgcgc
     2761 ccctgcccaa ccagcccgtc taattcccgc ccggtctcct cgacccctac accgtctggg
     2821 ccctctgctc ggcgccccgc gcccctactc atcgtcccac gccggccaca gccgccccct
     2881 cgatcttcga catcgaccca tctattagac tattgacagt aaacagtaaa aactcaaccc
     2941 aaattcaccc aaatccctta cctttgacta ttgacagtaa acactcaacc tatgacctcc
     3001 tatgataaga tcctccatct cttcgagcag tccaacgagc tgaccgtcaa agaaatagtg
     3061 gataaactcg gcatctccaa acagatgacc cacctcgccg tcaaccgcct ccgccaggag
     3121 aaccggctgg aaaaactagg cagcccgccc aagacggtct accgctacct ttcccccgac
     3181 caccaggtag aagagccccc ggccgactac gacgccaccg acaaagacca acaatatctc
     3241 aacgccaatt tcaccgtcgt cacagatacc ggcaatctcc tcgatggcat cgccgccttc
     3301 gaatactggt gccgtcagcg gaaactgccc gtcgccaaaa ccctgaagga atacatcgat
     3361 accaaaaaga aatacgcccc ttactacgac gaggagggca tggtcaacgg cctcgaaaaa
     3421 ctaaagaaca caaagggata cgaccacatc tggctggacg atctctgcta tctcgatttc
     3481 tacgccatcg aacgtttcgg caagacccgg ctcggcacca tcctgcacta cgccaaacag
     3541 ggccagaaca aattcctgat gcggctcatg atggaactca tcggcccccg gctaaaaacc
     3601 tttctccagg cacagggcgc ggacgccgtc gggtttattc ctccgacgat tcgccgcgaa
     3661 gtgcaattga tgaaagtcat ccaggcgcaa ctcaacatcg cgctgccggt ggtggagata
     3721 aagaagatca gcggactcat ccccgtgccg caaaaatccc tgtccaaact cgatgagcgt
     3781 attcgcaacg cggagaatac ctttgtcgtc accgatcgcc ggcagttccg tcatgtcgtc
     3841 ctcatcgacg acgccgtggg ttcgggctcg acgctaaacc aggtggccgc caaaatgaag
     3901 aacaagggca tcgcgcaaac gatcaccggt gtcgccatcg tcggcagctt taaaggattt
     3961 gacgtaatta cagacattta aacccatctc catgaccaac accgtcccga ccaataccat
     4021 ccccgccgcc acgaacgcag ctactctcta tctcataccc tgctggctgg aagaacagat
     4081 catcactccc ctccccgtct atatcctgga cgcggtgaaa caatgcaccg ttttcttcgt
     4141 ggagaacgaa cgcagcgccc gccgctatct gaaggcgatc tggaaagaga tggtcatcga
     4201 cgactatgaa tggtatccga tccacaaggc ggaggaagaa gtaaaggcca ccttccgcca
     4261 aaagctaaag gaaggcagga ccatcgggct catcagcgaa gccggctgtc cgggcatcgc
     4321 cgatccgggc cagatcctca cagaagccgc ccaccaggcc ggcgccaccg ttcgtcccct
     4381 cgtcgggccc agcagcatcc tcctcgccct gatggccagc ggcctcaacg gccagcaatt
     4441 ccgcttcacc ggctacctcc ccgtcgacaa cagcggccgc gccaaggccc tcaaggatct
     4501 cgagaccgaa tcacagcgcc tgcactgcac catgctcttt atcgagaccc cgtatcgcaa
     4561 caaccaactc ctcgaaaccc tcctcgccac ctgccgcccc accacccggt tatgcatagc
     4621 cgccaacctc accgcgccga ccgaatatat ccgcaccctg cccgtcgccg aatggaggaa
     4681 gctgccaaaa cccgccgccg catccaaccc gggcactccc tccaccgccg cgcccggccc
     4741 caccggcccg cacagccccg cccactccgc gcccggcaac ccgactgcgc atcaggccac
     4801 cctccccgac ctccacaaac aacccgctat tttcgcgtta ttagcatagt cggcgccaaa
     4861 ctaccctcat ttcgtgcgaa aaaatcacaa aacaaccgtc cttttatgta caaaatatcc
     4921 aaaaactacc cccattttgt gccacaaaat cgataaaaca accgtacttt tgtgcacata
     4981 ctcacataaa tgtttaatcg tcaagtcatt gaacaactgg aagattggaa gcaccaatcc
     5041 aatcgcaaac ccttaatcct tagaggcgca cgacaagtgg gaaaaacaac ggcggttaac
     5101 gcatttggcc ggcgatttcc tcagtacatc tatttcaacc tggaagacgc ccccgatagg
     5161 gaaatattcg ctgaaaatgc ggacatccat cgactggtcc aaactatatt cttgcggaaa
     5221 agcaagtcac tcgccaggcg gagcgaaacg ctcatcttta tagatgagat tcagcaattt
     5281 cctcaagccc tcaatatgct ccggtatttt tatgaacaat atcccgaaat tgccgtcgtc
     5341 gctgccggat cgttactgga aactatcttt gccaaaggaa tgagcttccc ggtcggccgg
     5401 gttgatttta tggtgatcag gccggtcggt ttccccgaat tcctgggtgc catcgaccag
     5461 gagcaagttc ttgaacagtt gctgcacgtc ccgatgaatg atttcgccca ttctacgctg
     5521 ttacaactat tccatacata cacgctcgtc ggtggcatgc cagagatcgt ggacgagtat
     5581 gcacggacaa aggatcttac ggctctgtcc gccatttatg aacggttaat caccgcctat
     5641 gtggacgacg ttgaaaaata tgcgacgaac gatcatttcg ttcaggttat caggcactgc
     5701 atccgggcga gtttcctgga cgcaggtacc cgcatcaaat ttcagcattt cggccgctcg
     5761 aactacaatt cccgggaagt cggtgaggca ttgcgcaccc ttgagaaaac ctacctcctg
     5821 tccctcctct atccaacaac agatgccgtc ctgccggtac gtcaggattt aaaaaggtct
     5881 ccccgattac atgtcctgga cactggcatg atgaactatt ttcttggcct cagaacggag
     5941 gtgttgggca cagccgatct caacagcatt taccagggtg tcatcgttga gcacctggtc
     6001 ggccaggagc tgctggcaac ccagtttaat gtccttagcc ggttagattt ttgggtgagg
     6061 gaaaaagcgg gctcttccgc cgaggtcgat tatgtctatc cattcg
//
LOCUS       DATGFG010000982         4086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_900425, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000982 DATGFG010000000
VERSION     DATGFG010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900425"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VKQ52_18565"
     CDS             complement(<1..321)
                     /locus_tag="VKQ52_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89269.1"
                     /translation="MYLEQRIDLLVRLGEYCLSPAPAWEAARQRAHALNGWFIPEFID
                     EAVRQIVTQYLQRDKLEAWVRDYALPPSRQVPRTVGVVMAGNIPLVGFHDLLSVFLSG
                     HRQLI"
     gene            408..755
                     /locus_tag="VKQ52_18570"
     CDS             408..755
                     /locus_tag="VKQ52_18570"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLZ89270.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEGGVEWAIADGSSVKGSFT
                     LLDGTVIDANHRNPPISVDNYYIVPNKCTECQGFHEEPQCAAVCPVDCCVPDEMYRET
                     VEELLAKKDRLHL"
     gene            984..1979
                     /locus_tag="VKQ52_18575"
     CDS             984..1979
                     /locus_tag="VKQ52_18575"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HLZ89271.1"
                     /translation="MKPIIAFVTGGYSGESVISYKSAITIQNNLDHDRYTVYRIDITP
                     ESWFHETADGNKIEVDKNDFSLTIAAKKIRFDAVLIGIHGTPGEDGKLQGYFDLMGLP
                     YTSCDAAVSALTFNKRFTVAVAAFNGINVARSALLIKGRIDSPEEVTRHLQFPVFVKP
                     NNGGSSIGMSKVNNPSEELGPAVEKAFREDNQVLVEEFIAGREFTIGVFRAKGEIITL
                     PFTEVKSKKEFFDFEAKYTAGMNEETTPAIVDEAVAAKVRAAAKKIYQVFNCSGIIRI
                     DFIYNEAKGEPYMLEINTVPGQSEASIVPQQLKVFGWTLKEFYSALVEEALGRRQ"
     gene            2026..2850
                     /locus_tag="VKQ52_18580"
     CDS             2026..2850
                     /locus_tag="VKQ52_18580"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HLZ89272.1"
                     /translation="MFKFLTSKPLWVNVLAGVVLLLLLLLIFLGSLALLTQHGKTMKI
                     PSVTGLSLDEAKKSLQSQGFEIQIQDSVYSDTMRPLQVVKQFPEADNLVKINRTIYLT
                     INRAQAPFIQMPNLVSMSFRNAEMILRQYGLKLGDTIFKPDFARNSVLEQQYKGEAIK
                     PGTQIQQGSSITLILGNGIGGGQGFIVPDLFGLTYLQAKARLDSMGLSFGAVVPGKDV
                     RDSAEAYIFWQDPPQMNQDGLPNRIHPGQSIDIKLQAQRPFRGDSAQQSPPPGGDN"
     gene            2880..3188
                     /locus_tag="VKQ52_18585"
     CDS             2880..3188
                     /locus_tag="VKQ52_18585"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLZ89273.1"
                     /translation="MQAISVEEVKARLDAGETLHLVDVREPAENAEFNIGGVLLPLGR
                     VRMMDTDDIDKWKDEEVICYCRSGNRSGQACMILETAGFTNIKNLTGGMLAWMDRIGS
                     "
     gene            complement(3215..>4086)
                     /locus_tag="VKQ52_18590"
     CDS             complement(3215..>4086)
                     /locus_tag="VKQ52_18590"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLZ89274.1"
                     /translation="RLTRHTEAFLAPLALATAEGFQSKSADGTMVSGILYRPAGSVGA
                     TGAAGQPGATQSGAGQSAAGKMPLILYIHGGPVSQDVFDFDLSRQMLAGGGFAVAAVN
                     YRGSNGRGLAYSKIISADWGNKEVIDLHGAVDWLVKQGIADADHLGVAGWSYGGILTD
                     YLIASDNRFKAASSGAGTGFTLSVYGVDQYINQYNNEIGAPWKAIDKYLKISYPLLKA
                     DRIRTPTLFMSGEKDFNVPTAGSEQMYQALRTLGVPTELIVYPGQFHGITVPSYQRDR
                     LERWLAWFGKYVK"
BASE COUNT          982 a         1275 c         1007 g          822 t
ORIGIN      
        1 gatcagttgc cggtgcccgg ataggaatac ggatagcaga tcgtggaaac ctaccagggg
       61 gatattcccg gccatgacca cccccaccgt ccggggcacc tgccgggagg gcgggagagc
      121 atagtcccgt acccaggctt ccagtttgtc ccgctgaagg tattgggtaa cgatctgccg
      181 caccgcctcg tcgatgaact ccgggatgaa ccatccgttg agggcatgcg cccgctgccg
      241 ggcagcctcc caggccggag ccggcgaaag acagtattcc cccaacctta ccaataaatc
      301 tattcgttgt tctaaataca taaatccata ttatatttgc gaggcggacc ataatccgca
      361 atcagatcgc aagttatatt tttaatcact ccctaattct caaaaaaatg gctattaaga
      421 taacagaaga atgcatcaat tgcggcgcct gtgagccgga gtgccccaat aatgctatct
      481 acgaaggcgg agtcgaatgg gcaatcgccg acggcagcag cgtaaaagga tccttcaccc
      541 tgctcgatgg tacggtcatc gacgccaacc accgcaaccc gcccatcagc gtcgacaact
      601 attatatcgt tcccaacaag tgcaccgagt gccagggctt ccacgaagaa ccgcaatgcg
      661 cggccgtctg tcccgtcgac tgctgcgtcc ccgacgagat gtaccgcgaa accgtggaag
      721 agctgctcgc taaaaaggat cgtttgcatc tgtagttttg tggataaaat gcggccggat
      781 aatttgaatt gtgatcggaa ttccctaact taggattagt aataagtgtt tgttaaagta
      841 gccctcgtgc tacttaaaaa taggcgggtg atatttcccg tcgtaaaagg gtgaaggaga
      901 ttgaagaaac cttcaccttt cttatttgta tcatatacca ttctcctcgc cttttcgtta
      961 ttttgccaaa attacttcca ttcatgaagc ccatcatcgc tttcgttacc ggcgggtatt
     1021 ccggcgaatc ggtcatctct tataaaagcg ccatcactat ccagaacaac ctcgaccatg
     1081 accgctacac cgtctaccgt atcgatatca cgcccgaaag ctggtttcat gaaacggccg
     1141 atggaaacaa gatcgaagtc gacaagaacg atttctccct caccatcgcc gccaaaaaga
     1201 tccgcttcga tgccgtcctc atcggcatcc acggcacccc cggcgaagac gggaaactgc
     1261 aagggtattt cgatctcatg ggcctgccct ataccagctg cgatgccgcc gtatccgccc
     1321 tcaccttcaa caaacgcttc acggtggcgg tagccgcctt caacggcatc aacgtcgccc
     1381 gatcggcatt actcataaaa ggccgcatag acagtcccga agaggtgacc cgtcacctgc
     1441 aattccccgt cttcgtcaaa cccaacaacg ggggcagcag catcggcatg tccaaggtca
     1501 acaacccctc cgaagagctc ggccccgccg tcgaaaaagc cttccgcgaa gacaaccagg
     1561 tgctggtaga ggaatttatc gcgggccgcg aattcaccat cggcgtcttc cgcgccaaag
     1621 gtgagatcat caccctcccc ttcacggagg tcaaaagcaa aaaggaattc ttcgatttcg
     1681 aagcaaaata cacggccggc atgaacgagg agaccacccc cgccattgta gacgaggccg
     1741 tcgcggccaa ggtaagggct gccgccaaaa agatttacca ggtctttaac tgtagcggca
     1801 tcatccgcat cgattttatc tacaacgagg cgaagggtga accttacatg ctggagatca
     1861 ataccgtccc cggccagagc gaggctagca tcgtgcccca gcaactaaag gtctttggct
     1921 ggacattaaa ggagttctat tccgctctgg tagaagaggc gctgggccgg cgccaatgat
     1981 cagccgacac ggccagaaac gtaaaaaaac gtattttaca gcactatgtt taagttcctt
     2041 acatccaaac cgctctgggt caatgtgttg gcaggtgtcg tgctcctctt gctcctcctg
     2101 ctgatcttcc tgggctccct cgccctcctg acccagcacg gtaaaacgat gaagatacca
     2161 tccgtcaccg ggctctccct tgatgaagca aaaaaatcgc ttcaatccca ggggttcgag
     2221 atccagatcc aggactcggt ctattccgat acgatgcgcc ccctgcaagt ggtgaagcaa
     2281 ttcccggaag ccgataacct ggtaaagatc aaccgcacca tctacctgac gatcaaccgc
     2341 gcccaggcgc ccttcatcca gatgcccaac ctggtgagca tgagcttccg caatgcggaa
     2401 atgatcctcc ggcaatacgg cctcaaactc ggggatacca ttttcaaacc cgacttcgcg
     2461 cgcaactccg tcctcgaaca acaatacaaa ggggaggcga tcaaacccgg tacccagatc
     2521 cagcagggta gttcgatcac cctcatcctg gggaacggca tcggcggcgg ccaggggttt
     2581 atcgttcccg atctctttgg gctcacctac ctccaggcga aggccaggct cgactcgatg
     2641 ggactttcct tcggcgccgt tgtgcccggt aaggatgtcc gcgactcggc cgaagcctat
     2701 atcttctggc aggacccgcc ccagatgaac caggacgggc tgcccaaccg tatccacccc
     2761 ggccaaagca tcgacatcaa gctccaggcg cagcggccgt ttcgtggcga ctcggcccaa
     2821 caatctcccc ctcccggcgg tgataattaa taacaaacga atcagtaaaa aataaggtta
     2881 tgcaagcgat ctctgttgag gaagtaaagg cccggctcga cgccggcgaa acgctccacc
     2941 tggtggatgt acgcgaaccc gccgaaaacg ccgaattcaa tatcggcggt gtcctgctcc
     3001 ccctgggccg ggtccgcatg atggatacgg acgatatcga taaatggaag gatgaagagg
     3061 tcatctgcta ttgtcgcagc ggcaaccgca gcggtcaggc atgcatgatc ctcgagaccg
     3121 cggggttcac caacatcaaa aacctcaccg gcggcatgct cgcgtggatg gataggatcg
     3181 gttcctgata atggcccgga gtgccatcgg ctccctattt tacatatttt ccaaaccacg
     3241 ccaaccatct ttccagcctg tcccgctggt acgacgggac cgtgatccca tggaactggc
     3301 cgggataaac gatcagttcg gtcggcacac ccagcgtccg caacgcctgg tacatctgct
     3361 cgctgcccgc cgtcggcacg ttaaaatcct tttccccgct cataaagagg gtaggggtcc
     3421 tgatcctgtc cgccttcagc aaaggataag agatcttcaa atacttgtcg atcgccttcc
     3481 acggcgcacc gatctcgtta ttatactgat tgatatactg atcgaccccg tacacggaaa
     3541 gcgtaaaacc cgtcccggcg ccgctgctgg ccgccttgaa ccggttgtcc gacgcgataa
     3601 gatagtctgt cagtatcccg ccatagctcc agcctgctac cccgagatga tccgcatcgg
     3661 cgatcccctg cttcaccagc cagtccaccg caccatgcag gtcgatcact tccttattgc
     3721 cccagtccgc ggagatgatc ttgctatagg caagcccccg gccattgctg ccccggtagt
     3781 tcacggccgc gacggcaaaa ccgccacccg ccagcatctg acgcgacaga tcaaagtcga
     3841 agacgtcctg gctcaccggg ccgccatgga tatacaggat aagcggcatt ttgcccgccg
     3901 ccgactgacc agccccagac tgagttgccc caggctgacc cgcggcaccc gtcgccccca
     3961 cactccccgc cggccgatac aggatacccg agaccatcgt tccatccgcg ctcttcgact
     4021 ggaagccctc cgccgtcgcc aacgccagcg gcgccagaaa agcctccgta tgccgcgtaa
     4081 gccgcc
//
LOCUS       DATGFG010000983         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1433758, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000983 DATGFG010000000
VERSION     DATGFG010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433758"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VKQ52_18595"
     CDS             complement(<1..1113)
                     /locus_tag="VKQ52_18595"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLZ89275.1"
                     /translation="MHFERKNCSNEELVRLYKSILLPRMIEEKMLVLLRQGRISKWFS
                     GIGQEAIAVGATLAMDAEEWILPLHRNLGVFTSRGMPLSKLFLQWQGSPDGYSKGRER
                     SFHFGSREHAICGMISHLGPQLGVADGIALAYKLQKRNRAALVFSGEGGTSEGDFHEA
                     LNVASVWDLPVIFLIENNGYGLSTPVSEQYRCESLVYKAEGYGMEGVRIDGNNILTVH
                     DTLRGIREYCIKYQRPYLVECMTFRMRGHEEASGIKYVPQELLEDWGKKDPVANFEGF
                     LREQGVLDDQAMERMREEIRHTIESELSRVSGPVTLVPDTEEELEDIYAPAPVVSGEP
                     AGERPERRLIDAISEGLDQSMEQHENLVLMGQDIAEY"
     gene            complement(1127..1480)
                     /locus_tag="VKQ52_18600"
     CDS             complement(1127..1480)
                     /locus_tag="VKQ52_18600"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLZ89276.1"
                     /translation="MNFLLRIVITAVVAFALSSVLPGIHIDTFWTAIVLSLVLAILNF
                     FLKPILIILTLPITILTLGLFLFVINALIVWFASDLVRGFHVDGFGWALLFSLLLSLL
                     TSLLYKDRDREERRR"
     gene            complement(1530..>2034)
                     /locus_tag="VKQ52_18605"
     CDS             complement(1530..>2034)
                     /locus_tag="VKQ52_18605"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HLZ89277.1"
                     /translation="IRIARNFPNRHHPDFQRVQVLNNVFGGFFGSRLMANIREDKGYT
                     YGIYSYLVNNIQESALVISTEAGKEVCAPAIAEVYKEMELLREELIDEEELQMARNFT
                     IGSILGDLDGPFQVAGRWKNILLNGLDGNYFNEGVRIIKTIGPEELRELARKYLNPEA
                     FFELVVV"
BASE COUNT          487 a          644 c          457 g          446 t
ORIGIN      
        1 atattccgcg atatcctggc ccatcagcac gaggttctca tgctgctcca tcgactggtc
       61 caacccctcg ctgatggcat cgatcaatct cctctccggc ctctcccccg caggttcccc
      121 gctgacaacc ggcgccggcg cgtaaatatc ctccagttcc tcttccgtat cgggaacaag
      181 cgtaacaggc cccgacacac ggctcaattc cgattcgatc gtatgccgga tctcctcccg
      241 catccgttcc atcgcctgat catccagcac cccctgctcc cgcaggaaac cctcgaaatt
      301 agccaccggg tccttcttgc cccagtcctc gagtagctcc tgcggcacat attttatccc
      361 gctggcctcc tcatggccac gcatccggaa ggtcatgcac tccacgagat agggccgctg
      421 gtatttgata caatactccc ggatcccccg cagcgtatca tgaacggtga ggatattatt
      481 gccgtcgatg cggaccccct ccatcccata cccctcggcc ttatagacca ggctctcgca
      541 acgatactgc tcggagacag gggtactcag cccatagcca ttattctcga taagaaagat
      601 cacgggcagg tcccatacgg aagccacatt cagggcctcg tggaaatcgc cttcgctcgt
      661 accgccctca ccgctgaata ccagcgccgc ccggttccgc ttctgtaact tataggcaag
      721 ggcgatcccg tcggcgacac ccagctgcgg tcccaggtga gagatcatcc cgcagatggc
      781 gtgctccctg ctcccaaaat gaaagcttcg ctcccggccc ttgctatacc cgtccggact
      841 tccctgccac tgtagaaata attttgataa gggcatcccc cggctggtga ataccccgag
      901 attgcggtga agcggaagta tccattcttc cgcatccatc gccagcgtcg cacccacggc
      961 gatagcctcc tggccgatgc cgctaaacca tttgctgatc cgtccctgcc gcaataagac
     1021 cagcatcttc tcttcgatca tccggggcaa taaaatgctt ttatagaggc ggaccagttc
     1081 ttcgttgcta cagttttttc tttcaaagtg catcctggat gattttttat ctccttctct
     1141 cctccctgtc ccgatcctta tacaaaagcg aggtcagcag ggacaggagc aggctgaaca
     1201 aaagcgccca cccgaacccg tccacatgga atccgcgtac aagatcgctg gcaaaccaga
     1261 cgataagggc attgatgaca aaaaggaata gacctaaggt aagaatagtg atcggcaggg
     1321 taagaatgat caggatcggc ttcagaaaaa aattcaatat cgccaacacc agcgaaagca
     1381 cgatagccgt ccagaaagta tcaatatgaa tgcccggcag aacagaagat aatgcaaaag
     1441 caactaccgc tgtgataaca atgcggagca ggaaattcat gcgattcttt tgctgaaatt
     1501 acggtataaa taaactacca ccaactcaat tacaccacta ccaactcgaa gaaagcttcg
     1561 gggttgaggt atttccgggc cagctcccgc aactcttccg gcccgatggt tttgatgatc
     1621 cggacccctt cgttaaaata attcccatcc aggccgttga gcaggatatt cttccaccgg
     1681 ccggctacct ggaacggacc gtccaggtct ccgagaatgc tgccgatggt aaaattccgg
     1741 gccatctgca actcctcttc atcgatcagc tcttcccgta acagctccat ctccttgtag
     1801 acctcggcga tcgccggcgc acagacctct tttcccgcct ccgtactgat caccagcgca
     1861 ctttcctgga tattgttcac gagatagctg tagatcccat aggtataccc cttatcctca
     1921 cggatattgg ccatcagccg ggaaccaaaa aaaccgccaa acacattatt cagcacctgc
     1981 acccgctgaa aatccgggtg gtgccggttg ggaaaattgc gggcgatccg gatg
//
LOCUS       DATGFG010000984         5150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_855698, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000984 DATGFG010000000
VERSION     DATGFG010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855698"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VKQ52_18610"
     CDS             complement(<1..495)
                     /locus_tag="VKQ52_18610"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ89278.1"
                     /translation="MNRKLRSFLFLLFCFFCYHSLLAGKPAEYGTITGTITDKAGKPI
                     AGATITIPDLKTGTVSDENGKFTLTLGSRGIHLVEVSYVGYSTFYHNVDFSKTAQLDV
                     QLTLSTIETEEVVVTGVSRATEMKRAPIPIIAVGKEYLEQRSGATNIIDAVANLPGVS
                     AVTTG"
     gene            complement(581..931)
                     /locus_tag="VKQ52_18615"
     CDS             complement(581..931)
                     /locus_tag="VKQ52_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89279.1"
                     /translation="MHILYHCIVMLRRRKKIIAVLLLLVFSQKAGLRLWMHNWLHAGK
                     IASSCGGAASEKIQLACDCFNEAMLPLEETAVFTVSAPIQKSTELGYPPPSLVPDTEK
                     VFCSLKGPPALPSF"
     gene            complement(1045..1404)
                     /locus_tag="VKQ52_18620"
     CDS             complement(1045..1404)
                     /locus_tag="VKQ52_18620"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HLZ89280.1"
                     /translation="MKKTQILLALLFGLTTATIAQTKPQLTAVIKTPTVQCDQCKNRI
                     ERYMSHEEGIVKVNVDYKKKTTTVTYLTDRTNVENIKALIANVGYDADDVTAEPDAYK
                     KLPTCCKKPEDGGGKKQ"
     gene            complement(1538..2200)
                     /gene="pyrE"
                     /locus_tag="VKQ52_18625"
     CDS             complement(1538..2200)
                     /gene="pyrE"
                     /locus_tag="VKQ52_18625"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HLZ89281.1"
                     /translation="MAEKIITTNERAVAERLLQVQAIRLSVQQPFTWSSGWKSPIYCD
                     NRKVLGFPYIRDFIKSELCNVIFAQFPEAGLLAGVATAGIAWGAMAADQLKLPYVYVR
                     SKPKEHGLGQQIEGYYEKNQPVVVIEDLISTGKSSLQVVDILRNEGLEVIGLVSIFNY
                     GFTAAREAFEKAGVPYTSLSNYPTLISLALEKGLVSPKEEDILLQWSANPADWQGPKA
                     NH"
     gene            2255..2878
                     /locus_tag="VKQ52_18630"
     CDS             2255..2878
                     /locus_tag="VKQ52_18630"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLZ89282.1"
                     /translation="MYIKIYFNDKPLFLCDEMTAEIREFAHHDDAVLIDEFSHPAVNS
                     MIHEMRQSKVHAGIFIHKDLEELRKAFWKKFLLVKAGGGLIRNGEEKYLFMLRRGVWD
                     LPKGKLDPGETIEQCAIREVMEETGIGGVILDGALLVTYHTYDESGKHILKETHWFRM
                     LAPAPGELVPQTEEQITELRWVDRDGMKELLRRTFPSIRDVIRAVGL"
     gene            complement(2939..3370)
                     /locus_tag="VKQ52_18635"
     CDS             complement(2939..3370)
                     /locus_tag="VKQ52_18635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HLZ89283.1"
                     /translation="MSIWFDPTVKPEQLHPLGHNTMGEHIGIVFTEIGDDYLKATMPV
                     DNRTRQPYGLLHGGASVALAETIGSVGCALTIDQSKFICVGIEINANHLRGVREGLVT
                     GIAKPLHIGKTTHVWEIKLYDDREKLVCVSRLTVAILKKEA"
     gene            complement(3382..3897)
                     /gene="coaD"
                     /locus_tag="VKQ52_18640"
     CDS             complement(3382..3897)
                     /gene="coaD"
                     /locus_tag="VKQ52_18640"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HLZ89284.1"
                     /translation="MQRIGLFPGTFDPITIGHQDIINRSLPLFDKLFIGIGRNANKVA
                     MFSEEQRLGWIREIYKDNPKVDAVIYEGLTIRCCQQVGARFILRGIRYVNDFEYEKAI
                     ADMNRSLDTNIETIFLTCLPQYTSVASTLVRDVLKNGGDVMQFLPEVVQRSLSGKNFS
                     DKSATPHRRTY"
     gene            complement(3918..4844)
                     /locus_tag="VKQ52_18645"
     CDS             complement(3918..4844)
                     /locus_tag="VKQ52_18645"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HLZ89285.1"
                     /translation="MQLSTKHLLGIRDLQPGDIQLILDTATQFKEVLQRPIKKVPTLR
                     DVTIVNLFYENSTRTRISFELAEKRLSADTINFTASGSSAAKGETLLDTVNNILSMKV
                     DMVVMRHSASGAPHFLAKHIPAAIVNAGDGINEHPTQALLDAFSIREKLGGLAGKKIA
                     IVGDIMHSRVALSNIYLLKKMGAEVTIAGPPTLIPVYIREAFDVRVEYNLRKALEWCD
                     VANILRIQLERQNQPLFSSLREYSLAYGVNKRLLDSLSKEIVIMHPGPINRGVELDSD
                     AADSKQSIILHQVENGVAVRMAVLYLLAGGTQ"
     gene            complement(4894..>5150)
                     /locus_tag="VKQ52_18650"
     CDS             complement(4894..>5150)
                     /locus_tag="VKQ52_18650"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HLZ89286.1"
                     /translation="IENKEVVLIDDVLWTGRTIRAALDALLDFGRPAKVELCVLIDRR
                     FSRQLPIQADYVGRPIDSIITQKVKVYWTEKDGKDEVALL"
BASE COUNT          994 a         1105 c         1744 g         1307 t
ORIGIN      
        1 tccggtggtc accgcactga cgcccggaag gttggccacg gcgtcgatga tattggtcgc
       61 gcccgagcgc tgctccaggt attccttgcc aacggcgatg atgggaatgg gcgccctttt
      121 catttctgtg gccctgctga cgcccgtgac caccacttct tccgtctcga tggtggataa
      181 ggtcaattgt acatcgagtt gggcggtttt actaaagtct acgttgtgat aaaatgtact
      241 atagcctaca taactaactt ccaccaggtg aatgcccctg gagccgaggg taagggtaaa
      301 ttttccgttc tcgtcggaca ccgtacctgt tttcaggtcg gggatcgtaa tcgtggcgcc
      361 ggcgatgggt ttgccggctt tgtccgtaat ggtacctgtt atcgttccgt attcagcagg
      421 cttccctgcc aggagggaat ggtagcaaaa aaagcaaaat agcaggaaga ggaatgagcg
      481 taacttccgg ttcataatcc gatgtgatta aacgatagaa aattgagatg tgcttcagac
      541 cggtcaggtc tgagagccac gggtgtgaaa aggtcaggag ttagaaggag ggaagggcgg
      601 gtggaccttt tagcgaacag aacacctttt cggtgtcagg aaccagtgat ggtggaggat
      661 atcccagctc agtagatttt tgtatggggg cagagacggt gaaaacagcg gtttcctcca
      721 gcggtaacat ggcttcatta aagcagtcgc aggccaactg gatcttttcc gaagctgcgc
      781 cgccacagga ggaagcgatc ttgcccgcgt gaagccaatt gtgcatccaa aggcgcagtc
      841 ctgctttctg gctgaatacc agtaagagta atacggctat aatttttttc cggcgcctga
      901 gcatgacgat gcaatgatac aaaatatgca acgatagtgc aaatttttta aagggctacg
      961 gccggccccg gatcccgaca gggattcgga cataaatgaa aagcccggct acggccgggc
     1021 ttttgctata catgggaata ggtgttattg ttttttgcca ccgccgtctt cgggcttttt
     1081 gcagcaggtg ggtaattttt tgtaggcgtc gggttcggcg gtgacgtcgt cggcgtcata
     1141 gcctacgttg gcgatgaggg cctttatatt ttcgacgttg gtgcgatcgg tgagataagt
     1201 gacggtggtt gttttctttt tgtagtcgac atttaccttg acgatccctt cttcgtggct
     1261 catgtagcgc tcgatgcggt tcttacattg gtcgcattga acggtggggg tttttatgac
     1321 ggcggtgagt tggggttttg tctgggctat cgtggcggtg gtgagaccga agaggagggc
     1381 aagaaggatc tgcgtttttt tcatacgtaa tgggattgta tattaaatat acggattttt
     1441 ttggtccgaa gttgccttaa ttatatgcct ttcctgtcgg cagggtggtt tgcaccctgg
     1501 ggcgaaagaa gaaatatcgc cccggcgcaa accacggcta atgattggcc ttcggcccct
     1561 gccagtcggc aggattggcg ctccattgga ggaggatgtc ttcttctttg gggctgacca
     1621 ggcctttttc gagcgcgagg ctgatgaggg tggggtagtt ggagagggag gtgtagggga
     1681 cgccggcttt ttcgaaggct tcccgggctg ctgtgaagcc gtagttaaag atggatacga
     1741 ggccgatgac ttcgaggcct tcgttgcgga ggatgtctac cacttgcagg ctgcttttgc
     1801 cggtggagat caggtcttcg atgacgacga cgggctggtt cttttcgtag tatccttcga
     1861 tctgttgccc gaggccgtgt tcttttggtt tactccggac gtagacgtag gggagtttta
     1921 gctggtcggc ggccatggcg ccccaggcga tgccggctgt ggcgacgccg gcgaggaggc
     1981 ctgcttcggg gaactgtgcg aagatgacgt tgcagagttc ggattttatg aagtcgcgga
     2041 tataggggaa gcccaggact ttgcggttat cgcagtagat ggggcttttc cagccggagg
     2101 accaggtgaa gggttgttgg acgctgaggc ggatggcctg tacctgcagg agccgttcgg
     2161 cgacggcccg ttcgttcgtg gtgatgatct tttctgccat gacgcgaaag tagtaaattg
     2221 tgctgaaccg gatggaaccg gccgaaaatt ccatatgtat atcaagatct actttaacga
     2281 caagcccctg ttcctgtgtg atgagatgac ggcggagata agagagttcg cccatcatga
     2341 cgatgcggtg ctgatcgatg agttctcgca tccggcggtg aattcgatga tccatgagat
     2401 gcggcagtcc aaggttcatg cggggatctt tatccataag gatcttgagg agttgcggaa
     2461 ggctttctgg aagaaattcc tgctggtgaa ggccggtggc gggcttatcc ggaatggcga
     2521 ggagaaatat ttattcatgt tacggcgggg ggtatgggat ctgccgaagg gcaagcttga
     2581 tccgggggag acgatcgagc agtgtgcgat ccgggaggtg atggaggaga cgggtatcgg
     2641 gggggtgatc ctggatgggg cgttgctggt gacgtatcac acgtatgatg agagtgggaa
     2701 gcatattttg aaggagacgc attggttccg gatgctggcg ccggcgccgg gggagctggt
     2761 gccgcagacg gaggagcaga tcacggaatt gcggtgggtg gacagggatg ggatgaagga
     2821 gttgttacgg aggacgttcc catcgatcag ggatgtgatc cgggccgtgg ggctctgatc
     2881 ggggtgatgg gcttttgatc cggacggtgg gtttttggtc cgggccgtgg gactttgatc
     2941 aggcttcttt ttttaagatc gcgacggtga ggcggcttac gcacacgagt ttttcgcgat
     3001 cgtcgtagag ttttatctcc cagacgtggg tggttttccc gatgtgtaat ggttttgcga
     3061 tcccggtgac gaggccttcg cggacgccgc ggaggtgatt ggcgtttatt tcgatgccga
     3121 cgcaaatgaa tttcgactgg tcgatcgtga gggcgcagcc gacgctgccg atggtttcgg
     3181 ccagggctac cgatgcgccg ccgtgcagga ggccgtaggg ttgtcttgtg cggttatcga
     3241 cgggcattgt tgccttcagg tagtcgtcgc ctatttcggt gaagacgatg ccgatgtgct
     3301 cgcccatggt attgtggccg agggggtgga gctgttccgg ctttacggtg ggatcgaacc
     3361 agatggacat aggtgttgtg gttagtaggt tctgcgatgg ggggtggcgg atttgtcgct
     3421 aaagttcttg cctgagaggg atcgttggac gacttcgggg aggaactgca tgacgtcgcc
     3481 gccgttcttc aatacgtcgc ggacgagggt ggaggcgacc gaggtgtatt gcggcaggca
     3541 ggtgaggaag atggtctcga tgttggtgtc gaggctgcgg ttcatgtcgg cgatggcttt
     3601 ttcgtattcg aagtcgttta cgtagcggat gccgcggagg atgaatcggg cgccgacctg
     3661 ctggcagcag cggatggtga ggccttcgta gatgacggcg tccacttttg ggttatcctt
     3721 atagatctcg cgtatccagc cgaggcgttg ttcttcggag aacatggcga ctttgttggc
     3781 gttgcggccg atgccgatga ataatttgtc gaacaggggc aggctccggt tgatgatatc
     3841 ctggtgcccg atggtgatcg ggtcgaaggt acccgggaag aggccgatac gttgcatagg
     3901 caagtagatt ggtatgttta ttgggttccg cctgccagga gatagaggac ggccatgcga
     3961 acggctaccc cgttttccac ctggtggagg atgatggact gtttgctgtc cgctgcgtcg
     4021 ctgtcgagct cgacgccccg gttgattggg ccggggtgca tgatcacgat ctccttgctt
     4081 aagctgtcga gtaaccgctt attgacccca taggcgaggc tgtactcgcg cagggaggag
     4141 aagagcggtt ggttttgccg ttcgagctgg atgcggagga tattggcgac gtcgcaccat
     4201 tcgagggctt ttcgcaggtt gtattccacc cggacgtcga atgcctcgcg gatatagacg
     4261 gggatgaggg taggggggcc ggcgatggtc acttcggcgc ccatcttttt cagcagatag
     4321 atattgctca gggccacccg ggagtgcatg atatcgccga cgatggctat ttttttcccg
     4381 gcgaggccgc ccagtttttc cctgatgcta aaggcgtcga ggagggcctg ggtggggtgt
     4441 tcgtttattc cgtcaccggc gttgacgatg gcggcgggga tatgttttgc caggaaatgc
     4501 ggggcgccgc ttgcgctatg gcgcatgacc accatatcga ccttcatgga gaggatattg
     4561 ttcacggtgt cgagcagggt ctcgcctttg gcggcggagg agccggatgc cgtgaagttg
     4621 atggtgtctg cggagaggcg tttttccgcc agttcgaagg agatgcgggt ccgggtggag
     4681 ttctcgtaga aaaggttgac gatggtcaca tcccggaggg tgggcacctt ctttatgggt
     4741 cgctgtaaaa cttccttgaa ttgggtggct gtatctaaaa tgagttggat atcacccggt
     4801 tggagatctc taatgccaag aagatgtttg gtgctcagtt gcattagaac gcaaaattaa
     4861 aggaaaattt acaatagggg ccggggattt ttatcagagg agggcgacct cgtccttacc
     4921 gtctttttcg gtccagtata ccttaacctt ctgggtgatg atggagtcga tggggcggcc
     4981 gacgtaatct gcctggatgg gcagttggcg gctgaagcgg cggtcgatga ggacgcagag
     5041 ttcgactttt gcggggcggc cgaagtcgag gagggcgtcg agggcggcgc ggatggtgcg
     5101 gcctgtccag aggacatcgt cgatgaggac gacttctttg ttctcgatgg
//
LOCUS       DATGFG010000985         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_854485, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000985 DATGFG010000000
VERSION     DATGFG010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854485"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VKQ52_18655"
     CDS             complement(<1..748)
                     /locus_tag="VKQ52_18655"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HLZ89287.1"
                     /translation="MQSASAGSPTAGGPSTPAASAPSSTYDFRGLNWADIHDNYSDSV
                     VVPSGLSSSDDYATVRSKAEIILTAFAQRGANTIRLPVNPFTVSGNWWNGYSGAIDQA
                     LANGMRVVLAYWEGTAAKDGLIDDTTRFWSMWQTVTAKYAGNANVWFEVFNEPFGYSA
                     TDLKTLYAQWLSRYPQIPAGRVLLDGVGYATGVNDIGADSRFGACLLSYHMYTWFDKS
                     KVTESDWEQAIGSLVYPDRTIVTEFGIPMTT"
     gene            complement(830..2491)
                     /locus_tag="VKQ52_18660"
     CDS             complement(830..2491)
                     /locus_tag="VKQ52_18660"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ89288.1"
                     /translation="MRTKYFILPVFLAVIFTTSCKKNYLNIVPTDRLSDVSILADSSL
                     FEDYVINRYLGVTLQNKEGEGTPPGFGRGFEYGMWSSLTDESIYNNDDNTWLIQRGQL
                     APENTGIAGTIWGRSYRSIRECNYALNNIASVQMSTLLRQRLAGELRFIRAFRYQDLI
                     RNYGGIILMGNKVLNLDSNMQDASLFKRSALKDCIDYASAELDTAASLLPQDNDASWV
                     LGRATKGAALALKSRLLLYAASPLYNVGTWADAAAAAKAVMNLGKYSLDASYSSLFLN
                     SSSNEIIFERLYAIGARHVCLEIANAPNGYGGWAGNTPLQNFVDAYQMKNGKDITDAS
                     SGYDSTNPYVNRDPRFYSTVLYNGASYRGSTVQVYTPGGKDSKDGPSNWNTTKTGYYL
                     RKYMDDNNPIDNPWNVAGLQPWIYFRYAEILLNYAEAQNEAVGPDGTVYSAINVIRER
                     AGMPDLAAGLSQSDMRTAIRHERQIELAFEEHRFYDVRRWKIADATENVPAYGVSVTL
                     SGGIYSYSRIVALSGRLFTSKEYWLPIPLSEIQASNNQLQQNTGY"
     gene            complement(2509..>3888)
                     /locus_tag="VKQ52_18665"
     CDS             complement(2509..>3888)
                     /locus_tag="VKQ52_18665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89289.1"
                     /translation="NSGSSYNFTRKSYISRIAYNFQEKYLLELQGRIDGSSTFPQGKQ
                     YGFFPSVSGGWRISKERWFDVPAISDLKLRGSYGTLGNDNVGLFQYFDNYSFNNQYVI
                     GGSIHPGIDVTKLANPNITWERARKLDIGLNATFLKNFTLEFIYFQQKRSDILTQRNA
                     SIPQVSGIVNPYSGDPLTPSENIGKVNSSGVEATLTYSNRAGDFTYGIGGNFTFAKSK
                     IIFIDEAAGVLSYQRQTGRPLNTYLLYQATGIFRSAADLAKYPHLTGAQVGDLIYKDV
                     DGDGQITAKDQLRSKYGNIPEITYGAVLNGGYKSWDLAVIFAGQADVSQYVLPESGTV
                     GNFYSSWADNRWSPTHLNGSYPRVDDRASSSINGGLYNSTFWMNNSAFIRLKSIQLGY
                     TVDNKLLSRAKIASLRLYVSAFNLFTITKVKDYDPEGTNGQTNGGLGATGQFYPQQRI
                     INAGVNVRF"
BASE COUNT          838 a         1071 c         1111 g          868 t
ORIGIN      
        1 ccgtggtcat cggtatcccg aattcggtaa cgatcgtgcg atcggggtag accagggagc
       61 cgatcgcctg ctcccagtca ctctccgtca ccttgctctt gtcgaaccag gtgtacatat
      121 ggtaagataa caggcaggcg ccaaagcggc tgtcggcccc gatatcgttg acccccgtcg
      181 catacccgac gccatcgagc aatacccggc cggcagggat ctgcggatac cggctcagcc
      241 actgcgcgta gagggtcttg agatccgtcg cgctataccc gaacggttca ttgaacactt
      301 cgaaccaaac gttcgcgttg ccggcgtatt ttgcagtaac ggtttgccac atgctccaga
      361 acctggttgt atcgtcgatc agtccatcct tcgccgcggt gccttcccaa taggccaaca
      421 ccacccgcat cccgttggca agcgcctgat cgatggcgcc gctatagccg ttccaccagt
      481 taccggacac ggtaaagggg ttcaccggca ggcgaatggt attcgcgccg cgctgggcaa
      541 aagcggtcag gataatttcc gcctttgacc gcaccgtggc atagtcatcc gacgagctaa
      601 gcccgctggg tacgacaacg gagtcagaat agttatcatg gatgtcggcc cagttcaatc
      661 ctctgaaatc atatgtactg ctgggggcag aagctgcggg tgtggacggt ccccccgccg
      721 tgggggagcc ggcggatgca gattgcaccc gccaactctt tgcacaggca gataaggtaa
      781 aaacccacac taaaaaaaaa catcttttca tcggtatcga tacccgggac taatatccgg
      841 tgttttgttg taattgatta ttggatgcct ggatctccga aagcggaatg ggcagccagt
      901 attccttcga cgtgaacaac cggccggaca acgcgacgat ccgcgaatag gaataaattc
      961 cgccggaaag cgtcacgctc accccatagg ccggcacgtt ctctgttgcg tccgcgatct
     1021 tccagcggcg tacgtcatag aaccggtgtt cttcgaaggc cagttcgatc tggcgctcgt
     1081 ggcggatagc cgtacgcata tcggactggg agaggcccgc ggccagatca ggcatccccg
     1141 cccgttcgcg gatcacattg atggcgctgt atacggttcc atccgggcct accgcctcgt
     1201 tctgcgcctc ggcatagttc aacaggatct ctgcataccg gaaatagatc cagggctgaa
     1261 gccctgcgac attccagggg ttgtcgatcg ggttgttgtc atccatgtat ttcctcaggt
     1321 aatagcctgt ttttgtcgta ttccagttgg aagggccgtc cttgctgtcc ttcccgccgg
     1381 gcgtatacac ctgtaccgtg ctgccgcggt agctcgcgcc attgtagagc accgttgaat
     1441 agaaacgggg atcgcggttg acataggggt tcgtggaatc atacccggaa gaagcatcgg
     1501 tgatatcctt gccgttcttc atctggtaag catccacaaa attctgcagg ggtgtattgc
     1561 ctgcccagcc gccatagccg ttgggcgcgt tggcgatctc gaggcagaca tgccgggcgc
     1621 cgatggcgta cagcctttca aagataatct cgttgctcga ggaattcagg aaaagtgacg
     1681 aatagctggc gtcgaggcta tattttccca ggttcatcac cgccttggcc gcggccgccg
     1741 catccgccca ggtgcccaca ttgtacaggg ggctggcggc atagaggagc agccgggatt
     1801 tcagagccag cgcggcgccc ttcgtggccc ggccgagcac ccaggaagca tcgttatcct
     1861 gcggcagcaa cgaggccgcc gtatcgagct ccgcggacgc atagtcgatg caatccttga
     1921 gcgccgaacg cttgaagagc gaggcatcct gcatattgct gtcgaggttg agcaccttgt
     1981 tccccatcag gatgataccg ccatagttgc ggatcaggtc ctggtaacga aaggcgcgga
     2041 taaaccgcag ctcaccggct aggcgctggc gaagcagcgt gctcatctgc accgatgcga
     2101 tattgttcag tgcgtagtta cactcccgga tgctgcggta gcttcttccc cagatggtgc
     2161 cggcgatgcc ggtgttctcg ggcgccagct gtccgcgctg gatgagccag gtgttatcgt
     2221 cattattata gatggactca tccgtcaggg aactccacat gccatattcg aagccgcggc
     2281 caaagcccgg gggagtcccc tccccttcct tgttctgcag ggtgacgcct aaataccggt
     2341 tgatgacata atcctcgaaa agcgaggaat cggcgaggat ggatacgtcg gacaggcgat
     2401 ctgtaggaac gatattgagg taattctttt tacaggacgt cgtaaagata acggccagga
     2461 aaacaggtaa aataaaatat tttgttctca tcttgtcttg attgaaaatt aaaaacgaac
     2521 attcacaccg gcgttgataa tgcgctgttg cgggtagaac tggccggtgg cgcccaatcc
     2581 tccattcgtc tggccattgg tgccttccgg atcatagtcc ttcactttcg tgatcgtaaa
     2641 caggttgaaa gcgctgacat agagccgcag ggaagcgatc ttcgcccggg acagcaattt
     2701 gttgtccacg gtatagccaa gctgtatgct cttcagccgg atgaaggcgg agttgttcat
     2761 ccagaacgtg ctgttgtaaa gcccgccgtt gatgctcgaa gaggcccggt catccacccg
     2821 gggatagctg ccgttgaggt gcgtggggct ccagcgattg tccgcccagc tgctgtagaa
     2881 gttgccgaca gtaccggact cgggaagaac gtactggctg acatcggcct gaccggcgaa
     2941 aatgaccgcc agatcccagc tcttgtatcc gccgttgagc accgcaccat aggtgatctc
     3001 ggggatattg ccgtacttgg agcgaagctg atccttggcg gtgatctgtc cgtcgccgtc
     3061 gacatcctta tagatcaggt cgcccacctg tgcacccgta aggtgcggat atttggcgag
     3121 atcggcagcc gagcggaaga taccggtcgc ctggtacaac aggtaagtat tcaaaggccg
     3181 gccggtctga cgctgatagg aaagaacacc ggctgcctcg tcgatgaaga tgatcttgct
     3241 cttggcaaag gtgaaattgc cgccgatgcc ataggtgaaa tccccggcgc ggttggaata
     3301 agtaagtgtc gcttccacac cgctgctgtt caccttaccg atattctcgg aaggcgtgag
     3361 cggatcgccg ctatacgggt tcacgatacc ggacacctgg ggaatggagg cattccgctg
     3421 cgtcaggata tcactgcgtt tctgctgaaa atagatgaac tccaacgtaa agttcttgag
     3481 gaaggtggca ttcagaccga tgtccagttt ccgcgcccgt tcccaggtga tgttcggatt
     3541 ggccagtttg gtcacatcga taccgggatg gatactcccg ccgatgacgt attggttgtt
     3601 gaaggagtag ttgtcgaaat attggaacag acccacattg tcgttcccca gtgttccata
     3661 ggaaccccgc agtttcaggt cgctgatcgc aggtacatcg aaccaccgtt ccttcgagat
     3721 ccgccagccg cccgacaccg aagggaagaa gccatattgt ttgccctgcg ggaaagtaga
     3781 ggagccgtcg atacgtccct ggagttcgag gaggtatttc tcctggaagt tatacgcgat
     3841 gcggctgata tagctcttac gcgtgaaatt gtaactgctt ccgctgtt
//
LOCUS       DATGFG010000986         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1080202, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000986 DATGFG010000000
VERSION     DATGFG010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080202"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VKQ52_18670"
     CDS             <1..481
                     /locus_tag="VKQ52_18670"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HLZ89290.1"
                     /translation="MIPFCRTVKELHLVQDTMREFGLVRGNEGLQLFLMAELPSNILL
                     ASAFAKHIDGFSIGSNDLTLLTLGLDRDSSLVAHIYDERDEAIKTMLRMLIRTAKEAG
                     VKVGICGQGPSDFPDFAQFLVEEGIDSISVTPDSIIKTVRAIHEVETAIKNIEYATT"
     gene            468..1415
                     /locus_tag="VKQ52_18675"
     CDS             468..1415
                     /locus_tag="VKQ52_18675"
                     /EC_number="4.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF002748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine decarboxylase"
                     /protein_id="HLZ89291.1"
                     /translation="MPPPDFFFVEVLTFFADLFKAPAGNWWGYVTNGGSEGNLYGLYL
                     ARELYPDGLVFYSEATHYSVQKNVQLLNLRSIVIRTNEGGEMDYHDLADMVQMHREKP
                     VIILANIGTTMTEAKDNLGEIKRILRRYAIKSHYIHCDAALAGTYSALLDLEPGFDFS
                     YGADSIAISGHKFIGSPLPCGAILVKKNYKDRIGKAIPYIGTMDTTISGSRNGHTPLF
                     MWYAIKRFGIEGLRTRAINCLSLAEYTVSKLQSIGIPAWRNPSAITVVFPAPSAEIRQ
                     KWQIATENGLSHIICMPGLRKELIDEFVEELVEQPVGVE"
     gene            1639..1965
                     /locus_tag="VKQ52_18680"
     CDS             1639..1965
                     /locus_tag="VKQ52_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89292.1"
                     /translation="MKTQYAQMEVLLITGTTFLSRDLWEKDESRQNSLSSENEQLEEA
                     CWNGLLQTLLPEIFAEPIGNSRLYLWEVKTAASFLQLDMGEAPTAARSPFSIVPNSFL
                     STQSYN"
     gene            complement(2198..>3223)
                     /locus_tag="VKQ52_18685"
     CDS             complement(2198..>3223)
                     /locus_tag="VKQ52_18685"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLZ89293.1"
                     /translation="KGLPPGIPVRCIRPADGQQDSALSVTDGFSMTVATADGTGEEYL
                     IQLGRKAEVYKSMSVYLDKGAVTIDAPGPDFKDAVITGGPAITDYNAYCDYFRNSPAV
                     AGQRELVKKANALYNAHDSLGYAALRPKLQQGDSIRQAVTIQWIQQHPGSPVSAYLLT
                     KELKGLDLDEKAAILAKLTPEAKNNGPARRLGNLIEANTLTAIGKPAPDFSQDDTAGN
                     PVSLRDFRGKYVLVDFWASWCGPCRAENPNVVAAFNKYKLRNFAVLSVSLDGGFTKKD
                     NWLRAIRKDGMPWTHVSDLQGWHNAVAQKYGVQAIPTNFLVDPDGIIVARNLRGEELD
                     KKLQEVF"
BASE COUNT          715 a          852 c          909 g          747 t
ORIGIN      
        1 gatgatcccg ttctgccgaa cggtgaagga attacatctt gtacaggaca ccatgcggga
       61 attcgggctt gtacgcggta acgagggact gcagcttttc ctgatggcgg agctgccgtc
      121 gaatatcctg ctggcgtcag cattcgcaaa acatatcgac ggcttttcca tcggctcgaa
      181 cgacctaacc ctgctgaccc tgggattgga cagggattca tcgctggtgg cgcatatcta
      241 cgacgaacgg gatgaggcga taaagacgat gctgcggatg ctcatccgaa ccgcaaaaga
      301 ggcgggtgta aaagtcggca tctgtggcca ggggccgtcg gatttcccgg attttgcaca
      361 attccttgtc gaagaaggta tcgattctat ttcggtgacg ccggattcaa taataaaaac
      421 agtaagagcc atccatgaag tggaaacagc cataaaaaat atcgagtatg ccaccacctg
      481 attttttctt tgtcgaagtg ctcaccttct ttgcggacct cttcaaggcc cctgccggca
      541 actggtgggg ctatgtcacc aacggaggct ccgaaggcaa cctatatggc ttatacctcg
      601 cgcgggagct ttaccccgac gggctggtct tttattcgga ggccacgcat tacagcgttc
      661 aaaagaacgt ccagttattg aatctccgaa gtatcgttat ccggacaaac gaaggtggag
      721 aaatggatta ccatgacctg gccgatatgg ttcagatgca ccgggagaag cccgttatca
      781 tcctggccaa tatcggcacg acgatgaccg aagcaaagga caatctcggg gagataaaac
      841 gtatcctccg ccggtacgcc ataaaaagcc attatatcca ttgtgacgcc gcactggcag
      901 gcacctacag cgcgctcctg gacctcgaac ccggttttga ttttagctat ggcgcggata
      961 gtatcgccat cagcggtcac aagttcatcg gctctcccct cccctgcggg gcgatcctcg
     1021 taaagaagaa ttacaaagac cggatcggca aggccattcc ctatatcggc acgatggata
     1081 ccacgatctc cggcagccgg aatgggcaca cccccttgtt tatgtggtat gcgatcaagc
     1141 gatttggcat agaagggctg agaacacgcg cgatcaattg tctttccctc gcggaatata
     1201 cggttagtaa gctacagtcg atcgggatcc ccgcctggcg gaatccctcg gctattacgg
     1261 tggtgtttcc tgcgccttcg gcggagatcc gccaaaaatg gcagatcgct accgagaacg
     1321 ggctgagtca tatcatttgt atgccgggcc tccggaagga attgatagat gaatttgtcg
     1381 aggaactggt cgagcagccg gttggggtgg agtgacatta ataacggcag agggacccgc
     1441 gaaagagcgg ttaaccggat ggttgccggg ggagtttctt cgggtacaga caaccattta
     1501 gggctaaggg gttcgcccgg gtttttgtag gatttataag aacattgctg gatttggctt
     1561 ccaaaaattg ggaaatcgcc cgtggcaggg gccatctttg ctttagcaaa cagttttaga
     1621 aacttcaaaa tcccggttat gaaaacccaa tatgcacaaa tggaagtcct tctgatcacc
     1681 ggcaccacct ttttatcccg ggacctgtgg gaaaaagacg aatcccgcca gaatagtctt
     1741 tcttcggaaa atgagcagtt ggaagaagcg tgctggaatg gcttgctgca aacactgctt
     1801 ccggagatct ttgcggaacc tatcgggaat agccgcctat acctttggga agtgaaaacc
     1861 gctgcctcct tcctgcagct cgacatgggc gaagcaccta ccgccgccag gagccccttt
     1921 tccattgtcc ccaattcttt tctttctacg cagtcttata actaacgctt atcgtggatg
     1981 tcgtggcgga tttttggcgg ataccggcca ggaaggcgat ggcaaaggtg atcatccgga
     2041 tgaataaaaa gcctccgcct gacgggcttt ggagaagccc cggcggaggc gaacatgaga
     2101 aaaactgaca cacggctttt attatccgcc accgatgccg gggtccacgt gggacgaagg
     2161 gcgtatcgag gtggggcatt gaacagtttg tatgcggtta gaatacctcc tgtaatttct
     2221 tatccagttc ttccccgcgc aggttgcggg cgacgatgat gccgtcgggg tcaaccagga
     2281 agttggtcgg gatggcctga acgccgtatt tctgggctac cgcattgtgc cagccctgaa
     2341 gatcggacac gtgggtccag ggcataccgt ctttgcgaat ggccctgagc cagttgtcct
     2401 ttttggtaaa gcctccgtcg agggatacgc tgaggacggc gaagttccgg agcttatact
     2461 tgttgaaagc cgccacgaca ttggggtttt ccgcccggca gggtccgcac caactggccc
     2521 agaagtcgac gaggacatac ttccccctga aatcccgcag ggataccggg ttgcccgcgg
     2581 tatcgtcctg gctgaagtcc ggggccggtt taccgatcgc cgtgagcgta ttcgcctcga
     2641 tgaggttccc gaggcggcgg gcaggcccgt tattttttgc ttcgggagtc agcttcgcca
     2701 aaatggccgc tttttcgtcg aggtcgagcc ctttcaactc ctttgtcagc aggtaggcgc
     2761 tgacgggaga gccgggatgc tgttgtatcc attggatggt cacggcctgc ctgatggagt
     2821 ctccctgctg caatttcggt ctcagggctg catagcccag cgagtcgtgt gcattgtaca
     2881 gcgcattggc cttcttcacc aattcccgct gcccggccac tgcggggcta ttcctgaaat
     2941 agtcgcagta agcgttgtag tctgtgatgg ccggccctcc ggtgatgacc gcgtccttaa
     3001 agtcgggacc gggagcatcg atcgtgaccg cgcctttgtc gagatagacc gacatcgatt
     3061 tatagacctc cgccttccgc cccaattgga taagatactc ctcccccgtg ccatccgctg
     3121 tcgcgacggt catactgaat ccgtcggtga cgcttagcgc cgagtcttgt tgcccgtcgg
     3181 cgggtcgaat gcagcgcacc ggtattccgg gcggtaaacc ttt
//
LOCUS       DATGFG010000987         3775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1194747, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000987 DATGFG010000000
VERSION     DATGFG010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194747"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VKQ52_18690"
     CDS             <1..292
                     /locus_tag="VKQ52_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89294.1"
                     /translation="VRKYGVGFTIAKTATGDKGYITLGQNGTAITYTWEYDFATDLWA
                     EKTPFEGPPRSGAVGFTLMNRGFVGTGVSQGNQSAYQDLREFFPNQIYNQFD"
     gene            292..738
                     /locus_tag="VKQ52_18695"
     CDS             292..738
                     /locus_tag="VKQ52_18695"
                     /inference="COORDINATES: protein motif:HMM:NF027575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HLZ89295.1"
                     /translation="MALSSQKTFRETFLGKWGLSILMLMVIICTFGFGFYKRWKWRHE
                     HVFLELKAIPVKNGWGYDILQNGLPWCHQDYIPAVTGYRVFQSKEDALAAGRIVYDKA
                     VAGQLPDITEQEMRSKGIFIPPLDSTARPPEDQMVRLKTKDTAGSK"
     gene            805..3402
                     /locus_tag="VKQ52_18700"
     CDS             805..3402
                     /locus_tag="VKQ52_18700"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HLZ89296.1"
                     /translation="MRIPVITSLLALCGGGLLAQPASTSKFDQHKAFDPLFYPSGSTV
                     FRSAGGAPGEKYWTNRADYTIHALLDTGGHALSGDVTVTYTNNSPDALPFLWLQLDQN
                     IYRQDSRGERTNPVTGGRWSNKKFTEGDVIRGVVLLVNGQELKADWLVSDTRMQIRLP
                     QALKAGGGVIRFRINYSFSVPEYGTDRMGRQLVRDGWVYEIAQWYPRMEVYDDVSGWN
                     VLPYLGQGEFYLDYGNIDYTVTAPSDMVVVGSGELLNPTEVLTPAQQSRMARARASDK
                     TVFIRTAGEVGDPKARLPRASLTWHFRCNQTRDVAWSASRAFIWDAARINLPEGRKAL
                     AQSVYPAESSDAAAWGRSTEFVKGCIELYSEEWFPFTYPVATNVAGIVAGMEYPGIVF
                     CGGNERGGGLWEVTNHEFGHNWFPMIVGSNERKYAWMDEGFNTFINGVDTRVFNKGEF
                     AHKADAQKGAQYMFNERSEAILTIPDALQAYNLGAAAYSKPAMALDLLRKYVLGEKRF
                     DYAFRTYVRRWAFKHPTPWDFFHCMENAGGEDLGWFWRGWILNNWKLDQGVKEVKYVE
                     SDPAKGAVITIENLEEMAMPVVLAIQQENGRTDTLTLPVEVWQHGPTKAIAFPSTSKI
                     KSIVIDPAHDFPDINPTNNTWTGQPAERPVPAGVSAASVMDKYFAAVGGKDKLMAVKD
                     VVIVATANVQGQQVIETRKFLAPDASVLDIELPDMKMHAIHLVVNTDSVSLTQMGQSP
                     KLDEATKKEIRENSTAFPELNFSNEGYTTQLTSIKNIDGTDAYELKVTTPSGHVNLYY
                     YAVGSGLRIREVRSTSQGSATTEYGDYRDVNGIKFPWHIDDNQGEVELDMKVQSVKVN
                     TGLTPGDLK"
     gene            complement(3455..>3775)
                     /locus_tag="VKQ52_18705"
     CDS             complement(3455..>3775)
                     /locus_tag="VKQ52_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89297.1"
                     /translation="YDGSDYYITQGSSRYKLARTLTGQLTTNFGGGSLSAGNAVTTTL
                     SVPGVAAGDVVTVNANSGAVNPPYILITAYSTTAGTVTIQAYNAGGAAVVLASDSYKV
                     RVIK"
BASE COUNT          809 a          997 c         1182 g          787 t
ORIGIN      
        1 cgtccggaag tatggcgtag gattcacgat tgccaagaca gccaccgggg acaaggggta
       61 tatcaccctg ggtcagaacg gtacggctat tacgtatacc tgggagtatg atttcgccac
      121 cgatctgtgg gcggagaaaa cgccgttcga aggtccgccc cgcagcggcg cggttggctt
      181 tacccttatg aaccgcggat ttgtcggcac cggggtaagc caggggaatc agtccgctta
      241 ccaggattta agggaattct tccccaacca gatctataac cagttcgact aatggcgttg
      301 tcgtcgcaaa agacttttcg ggagactttt ctgggcaaat ggggactttc gatcctcatg
      361 ctgatggtca tcatctgtac tttcggcttc gggttctaca aacggtggaa atggcgtcat
      421 gagcatgtct ttctcgaact caaggccatc cctgtcaaaa acggctgggg gtatgatatc
      481 ctccagaacg ggttgccctg gtgccaccag gattatattc ccgctgtgac cggataccgg
      541 gtctttcaat cgaaggagga tgcgctggcg gcgggcagga tcgtctatga caaggctgtt
      601 gcgggtcagc tgccggatat aacggagcag gagatgcgga gcaagggtat ctttatcccc
      661 ccgttggatt cgacggcaag gccgccggaa gatcagatgg tgcgactgaa aacgaaagac
      721 acggctggat ccaaatagtt ttttttgata tcatttatgg tggggaaccc cgcgcggtcg
      781 cgcggggttc tttatcttta ggatatgaga atcccagtaa taacgtccct gcttgccctt
      841 tgtggcggtg gcctgttggc ccagccggcg tcaacctcga aattcgatca gcataaagca
      901 ttcgatcccc tcttctatcc gtcgggtagt acggttttcc ggagtgccgg cggggcgccg
      961 ggcgagaaat attggaccaa ccgtgccgac tatacgatcc atgccctgct ggataccggc
     1021 ggtcacgcgc ttagcgggga tgtcacggtc acctatacca acaacagccc cgatgcattg
     1081 ccgtttctct ggctgcagct ggaccagaat atctaccggc aggattcgcg cggagagcgc
     1141 accaacccgg tgacgggcgg ccgctggtcg aataagaagt ttacggaagg cgatgtgatc
     1201 cggggggtgg tgctgctggt caatggccag gagctaaagg ccgactggct cgtcagtgat
     1261 acgcggatgc agatccggct gccgcaggcg ctgaaggccg gcggcggtgt catccggttc
     1321 cggatcaatt actctttttc cgtcccggaa tatggtacgg acaggatggg ccggcagttg
     1381 gtgcgggacg gctgggtcta tgagatcgcg caatggtatc cccgcatgga ggtctatgat
     1441 gatgtcagcg gctggaatgt gctgccctat ctcggtcagg gggaattcta cctggactac
     1501 ggcaatatcg attataccgt aacggcgccc tccgatatgg tggtggtggg ctccggtgaa
     1561 ctgttgaatc caaccgaggt gttgacgccg gcgcagcaaa gccggatggc gcgggcgcgt
     1621 gccagtgata agacggtgtt tatccgcacg gccggagagg tgggcgatcc aaaggcccgg
     1681 ctgccaaggg cctccctcac ctggcatttc cggtgtaacc agacccgcga cgtggcatgg
     1741 tccgcttcgc gcgcctttat ctgggatgcc gcccggatca atcttccgga aggcaggaag
     1801 gccctggccc agtccgtcta tccggcggag tcttcggatg cggctgcctg gggacggagc
     1861 acggaattcg tcaagggatg tatcgaactc tattccgaag aatggtttcc ctttacctat
     1921 cccgttgcta ccaatgtcgc cgggatcgtt gcggggatgg aataccccgg tatcgtcttt
     1981 tgcggcggta acgaacgtgg cggcgggtta tgggaagtga ccaaccacga attcggtcat
     2041 aactggttcc cgatgatcgt cgggtcgaac gaacgcaaat atgcctggat ggacgaaggg
     2101 ttcaacacct ttatcaacgg ggtggacacc cgggtcttca acaagggtga attcgcgcat
     2161 aaggcggatg cgcagaaggg ggcgcagtat atgttcaacg aacggagcga ggccatcctg
     2221 acgatccccg atgcactaca ggcttataat cttggcgccg cggcctattc gaaaccggcc
     2281 atggcgctgg acctgttgcg aaaatatgtg cttggcgaga agcgcttcga ctatgccttc
     2341 cgcacctatg tgcggcgttg ggcgttcaaa catcctactc catgggattt cttccattgt
     2401 atggagaatg ccggcggaga agacctcggg tggttctggc gcggctggat cttgaataac
     2461 tggaaattgg accagggcgt gaaggaggtg aaatatgtgg agagcgaccc cgccaagggg
     2521 gcggtgatca ctattgaaaa tttggaggaa atggcgatgc cggtggtgct ggccatccag
     2581 caggagaacg gccggaccga taccctgacc ctgccggtgg aagtctggca acacgggccg
     2641 acaaaggcca tcgcctttcc gtcgacctcg aagatcaaga gcatcgttat cgacccggcc
     2701 catgatttcc ccgatatcaa tcccaccaat aatacctgga ccggtcagcc cgcggaacga
     2761 ccggtccccg ccggcgtgtc ggccgcttcg gtgatggaca aatacttcgc cgccgtcggc
     2821 ggcaaggaca agctgatggc ggtgaaagac gtggtcatcg tcgccacggc aaatgtgcag
     2881 gggcagcagg tgatcgagac ccggaaattc cttgcgccgg atgcctccgt gctcgatatc
     2941 gagctgccgg atatgaagat gcatgcgatt cacctggtgg tcaatacgga tagcgtgtcg
     3001 ctgacgcaga tggggcagtc cccgaagctg gatgaggcga caaagaaaga gatcagggag
     3061 aattcgacgg cttttccgga gctcaacttt tccaacgaag gctataccac ccagctcacg
     3121 agcataaaga acatcgatgg gacggatgcg tatgagttga aggtgacgac gccatccggg
     3181 cacgtcaatc tttattatta tgccgtcggg agcgggctgc ggatccgtga agtaaggagc
     3241 accagccagg ggtccgccac taccgaatac ggggattacc gcgatgtgaa cgggatcaaa
     3301 ttcccctggc atatcgatga caaccaggga gaagtggagc tggatatgaa ggtacagtca
     3361 gtgaaggtga acacagggtt gacacccggg gacctgaagt agcaggccgc tcccggggcc
     3421 ttgccgaaaa cggggccttg ccgtaacagg ctccttattt gatcaccctg accttatagc
     3481 tgtccgacgc gaggacgacg gcggcgcctc ctgcattgta tgcctggatg gtaacggtgc
     3541 cggcggtggt cgaataggcg gtgatcagga tgtaaggcgg gttcaccgcc ccggaattgg
     3601 cattcaccgt tacgacgtct cccgcggcca cgccgggcac agacagggta gtcgtcacgg
     3661 cgttgccggc gctgagcgaa ccgccgccga aattcgtggt cagctgccct gtcagggtcc
     3721 tggccaattt ataccggctg ctcccctgcg ttatataata gtcggaaccg tcgta
//
LOCUS       DATGFG010000988         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_739261, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000988 DATGFG010000000
VERSION     DATGFG010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739261"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="VKQ52_18710"
     CDS             <1..1564
                     /locus_tag="VKQ52_18710"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLZ89298.1"
                     /translation="LYVANADNNCLAVFDVHEPGSAGSKGFIPVGWYPTAVKVIGKKI
                     WVANGKGLTSLANPNGPSPIGRRQEVNYQQGDLKKTQPVQYIAGLFKGAMSIIPEPDA
                     KTLGDYSAQVYQNTPYTKNGELTAAGEAGNPIPMKVGDTSPIKYVFYVIKENRTYDQV
                     LGDMREGNGDTSLVLFGERVTPNQHALAREFVLLDNFYVDAEVSMDGHSWSMGGYAND
                     YLEKNWVTSYGGRGGSYDGEGNRAIANNKGGFIWDHCQRAGVSYRTYGEFADDALKPN
                     IPVLNGHTCPYYKGWDLGFYDTARVTQWKRDFDSLVSAGALPHFNTVRLGNDHTEGLR
                     RGRLTPFAHVADNDLAVGQFIEYLSKSPVWKESVVFILEDDAQNGPDHVDAHRSPVYV
                     AGGMVKRHFVDHTAYSTTSVLRTLELILGIAPMSQYDAAARPMWRCFTTTADLTPFGS
                     LPANIDLQEKNTAVNEWSRKSSTIDLTKEDRVPDRLFNEILWKGIKGDNAALPAPSRA
                     AFVRGSTVKDTD"
     gene            complement(1613..1894)
                     /locus_tag="VKQ52_18715"
     CDS             complement(1613..1894)
                     /locus_tag="VKQ52_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89299.1"
                     /translation="MKTKLFVLAIIIAGSLAVQSASAQVHVTITDRDYPGYTYYTYPA
                     WRGHYRDRMYYEHYHHRFEREHHVYFHGRDFDHDRFERENHWHGDHDRH"
     gene            complement(1987..3132)
                     /locus_tag="VKQ52_18720"
     CDS             complement(1987..3132)
                     /locus_tag="VKQ52_18720"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HLZ89300.1"
                     /translation="MKNLKLELVRGLAALMVFFCHCGMNCQPLFFNPIYHSLTNWALE
                     AVIIFFVLSGVVIHISQLNRPKSPNQFLLNRLKRLYPQYLVGLLLGFALYGWDMKLFL
                     GHAFFLATLKGYIVQVIPTNPAVWSLTFEMFFYLLYYLYLRSGSNKGVMRVWIAASII
                     CVPLYYLRLHGIAGHLIAMFAFSSLWLLGYFLVKYIGLIPKPTLPQVFFSVGILMLSA
                     RIRYTSEYYCIIKCFIFSLVAIPLFTYCLSDRSRNKKQLKWYYLIGAYLLFVGLIQVY
                     SRSMGSSKFIYLFCPPAFYLCAVLLDRAAKIKALLLNIGGFLGNISYSLYIIHFPLLY
                     IIGTIIGITRPVYFLPVALVTVIPLAWLLESITQRLFMSRGKFSLTH"
     gene            complement(3254..3745)
                     /locus_tag="VKQ52_18725"
     CDS             complement(3254..3745)
                     /locus_tag="VKQ52_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PXPV repeat protein"
                     /protein_id="HLZ89301.1"
                     /translation="MKKVIILSAVIASSLIATKGYSQVFVSAHVGFRVPGPRVYVAPA
                     PVYAEPAPVYEQAPAYDGGYYAAPPVCEAEFPGYAYYDYPAWDGHYRDRFYFEHYRPY
                     FERDHRAYFYGRRFDHERWEHERGEYGHDRGWDHDRGWGHDRGWDHDRGWGHDREWHR
                     GRW"
BASE COUNT          973 a          859 c         1172 g          831 t
ORIGIN      
        1 cctgtatgtg gccaatgcgg acaataattg cctcgccgtg ttcgacgtgc atgaaccggg
       61 gtcggcgggc tcgaagggtt ttatccccgt gggttggtat cccacggcgg tgaaggtgat
      121 cggaaagaag atctgggtcg ccaatggcaa ggggcttact tcgttggcca atcctaacgg
      181 gcccagtccg atcggccggc ggcaggaagt gaactaccag cagggcgatc tgaaaaagac
      241 acaaccggtg cagtatatcg ccggtctttt caagggagcg atgagcatta tcccggagcc
      301 ggatgcgaag acgcttgggg actattcggc gcaggtctat cagaatacgc cctatacgaa
      361 gaacggggag ctgacggcgg cgggtgaggc cgggaatccc attcccatga aggtgggcga
      421 tacctccccg ataaaatatg tcttttacgt gatcaaggag aaccgcacct atgaccaggt
      481 gctgggcgat atgagggagg gcaacgggga cacgagcctc gtgttgtttg gggagcgggt
      541 taccccgaac cagcatgcat tggcaagaga atttgtgttg ctggataatt tttatgtcga
      601 tgcggaggtc agcatggacg gacatagctg gagcatgggt gggtatgcca atgattacct
      661 ggaaaagaac tgggttacga gttatggcgg gcgtggagga tcctatgacg gtgaaggcaa
      721 ccgtgccatt gcgaataaca aaggcgggtt tatctgggat cattgccagc gggcaggggt
      781 ctcgtaccgt acgtatggcg aatttgccga cgatgccctg aagccgaata tccccgtcct
      841 caacgggcat acctgtccgt actacaaggg ctgggatctg ggcttttatg atacggcgag
      901 ggtgacccag tggaaacggg atttcgattc gctggtgtcg gcgggggcct taccgcattt
      961 caatacggtg cgcctgggca atgatcacac cgaggggttg agacggggaa gattgacccc
     1021 atttgcccat gtggcggaca atgacctggc cgtggggcaa ttcatcgaat acctgagcaa
     1081 gagcccggta tggaaggaga gtgtggtgtt tatcctggaa gacgatgcgc agaatggacc
     1141 ggatcatgtg gatgcgcacc ggagcccggt gtatgtcgcc ggggggatgg taaagcggca
     1201 ttttgtggat catacggcct attcgacgac ttcggtatta cggacgctgg aactgatact
     1261 cgggatcgca ccgatgtccc aatacgatgc ggcggcccgg ccgatgtggc ggtgctttac
     1321 gacgacggcc gatctgacac cgttcggctc gttgcctgcg aatatcgatc tgcaggagaa
     1381 gaatacggcg gtgaatgaat ggtcgaggaa atcgtccact atcgatctta caaaagagga
     1441 ccgggttccg gaccggcttt tcaacgagat cctgtggaag gggatcaagg gggataatgc
     1501 cgcgctgccg gcgccgagcc gggcggcctt tgtgagggga tcgacagtaa aggatacgga
     1561 ttaaaataaa aaggcggcgc agggttccct gcgccgcttg ttttagttat gatcaatgac
     1621 gatcatgatc accgtgccag tggttttccc gttcgaagcg atcgtggtcg aaatcccgac
     1681 catggaaata gacatgatgt tccctttcga aacgatggtg gtagtgttca taatacatgc
     1741 ggtcgcggta atggccgcgc caggcgggat aggtataata cgtatatccc gggtaatccc
     1801 tgtccgtgat ggtgacgtgc acctgggcgc tggccgattg aactgccagt gagccggcga
     1861 tgattatagc gagaacaaac aacttcgttt tcataaaaca acattttacg ttagaagatt
     1921 ttatattacc tataggcatg caataaggat gcccggcggg taaaaaaacc cgtaaagttg
     1981 tttttatcaa tgtgttagcg agaactttcc cctgctcatg aacagtcgct gggtaatgga
     2041 ctccaggagc caggcgagag gaatgaccgt aacaagagca acggggagaa aatagacggg
     2101 cctggtgatg ccgatgatgg tgccgataat atataaaagg gggaaatgaa taatatacag
     2161 gctataggaa atattcccaa ggaacccgcc gatattcaat agcagcgcct tgatctttgc
     2221 ggcccggtcg aggaggacgg cgcagagata aaaggcgggg gggcagaaaa ggtaaatgaa
     2281 cttcgagctt cccatgctgc gcgaatacac ctgtatcagc ccgacaaaca acagataggc
     2341 gccgatgaga tagtaccatt tcaattgttt tttgttccgg ctcctgtcac tcaggcaata
     2401 ggtaaacagg ggaatagcga caagggaaaa gataaagcat ttgatgatac aatagtactc
     2461 cgaagtatat cgtatccggg cggaaagcat gaggatgcct acggaaaaga acacctgtgg
     2521 cagggtgggt tttgggatga gaccgatgta tttgacgagg aaatatccca gcagccagag
     2581 ggaggaaaag gcgaacatgg cgatcaggtg gccggcgata ccatgcaggc gcagatagta
     2641 aagcgggacg cagatgatac ttgccgcgat ccatacgcgc atcacccctt tgttactgcc
     2701 ggatcggaga tagaggtaat acagcaggta gaagaacatt tcgaaggtca gggaccatac
     2761 ggccgggttt gttggaatga cctgcacgat atagcctttg agggtggcaa gaaaaaaggc
     2821 gtgcccgaga aacagcttca tgtcccatcc gtagagcgca aatcccagga gtagcccgac
     2881 aaggtattgc ggatatagtc tttttaaccg gttgagaagg aactggttcg gcgatttcgg
     2941 ccggttgagc tgcgagatat ggatcacgac gccggataag acgaagaata tgatgaccgc
     3001 ttccagggcc cagttggtaa gggagtgata gatagggtta aagaacaggg gctggcagtt
     3061 catcccgcaa tgacaaaaaa ataccatgag ggccgcaagg ccccgtacca gctccagttt
     3121 taaatttttc atgttgtcgg gggtagtaat tcaatacaga attatccatg aaaataggta
     3181 tttaaaagtc tccggcaaaa aaaggccgcc cggctaaaag cgggcggcct gaatttctta
     3241 ttgccttggc ggattaccat ctgcctctgt gccattcacg gtcgtggccc cagccgcggt
     3301 catggtccca tccacggtcg tggccccagc cacggtcgtg atcccagcca cggtcgtgac
     3361 cgtactcgcc acgttcatgt tcccaacgtt cgtggtcgaa acgacggcca tagaaatagg
     3421 ccctgtggtc cctttcgaag taaggacgat agtgttcaaa gtagaaccgg tcgcggtagt
     3481 ggccgtccca ggccgggtag tcatagtagg cgtagccggg gaattcggct tcacaaacgg
     3541 ggggggctgc atagtaaccg ccatcgtatg cgggagcttg ttcatagacg ggtgcgggtt
     3601 cggcgtaaac aggggccggt gcaacataca cccggggacc gggaacacgg aaacctacat
     3661 gcgcacttac gaatacctgg gaataacctt tggtagcgat gaggctggag gctataacgg
     3721 ctgaaagaat aattaccttt ttcatatctg tagattttaa gttagcggat gtattgttgc
     3781 tttcttgttt acatccttgt atgtatagaa aggtaatgag cgcctggatt taatg
//
LOCUS       DATGFG010000989         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_432763, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000989 DATGFG010000000
VERSION     DATGFG010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432763"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VKQ52_18730"
     CDS             <1..351
                     /locus_tag="VKQ52_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89302.1"
                     /translation="KELYPELKCTIIFQPHLFTRTRDFADGFAASLDRADEVILLPIY
                     PAREQPIAGVTSELIAGKMKKAKITIADKKEVLAMIKETAERRDQAQLLITAGAGDID
                     MLIEPLRQLLESPL"
     gene            458..1465
                     /locus_tag="VKQ52_18735"
     CDS             458..1465
                     /locus_tag="VKQ52_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89303.1"
                     /translation="MAVKLNIRKVVSAVFWMVAGTGVLVLLIAAIRYRNSNVCKGFKI
                     GITGGSSTGDHALFIDKKGISDILTAAGAGKGQNKPIQSFDLRRLEAALGRNVWIKEA
                     QLFFDNNGILQVRVVEREPVARVFTRDGNSCYIDSSGVQLPLPDKLTARLPVFTGYPG
                     QKIMLHGGDSVLTMEILHLSDFIRRDSFWTAQIAQVAILPDKGFELEPEAGDQRIGFG
                     DGRDIDEKFHRLSLFYKEVLSRTGPGKYDRIDISYAGQVVATRRGTAHDRYDSTQGMN
                     NIRQMIRSAQQLQPDTIRQQNIRPLEHAIMTEQTPADLDLVPDAGDSSTQNRKKTTKK
                     K"
     gene            1474..2856
                     /gene="ftsA"
                     /locus_tag="VKQ52_18740"
     CDS             1474..2856
                     /gene="ftsA"
                     /locus_tag="VKQ52_18740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HLZ89304.1"
                     /translation="MNQEQPIIVGLDIGTTKIAAIAGRKNEYGKLEILGFGRANSNGV
                     KHGQVLNIDETIKAIQMALDNCYASNPNLEISEVYVGIAGHHIKSLQTRGDIVRQNTD
                     EEITRREIDQLVADQYKTYIPAGDQIIDVIPQEFTVDNFQNIPNPIGYGGVKVGANFH
                     IITGDKNAIRNINRAVEKSNLHTKDLVLQPLASAAAVMGQEDLEAGVAIVDIGGGTTD
                     LAVFYEGILKHTAVIPFGGENITNDIKTGLGVLKTQAEQMKVQFGSALSNEAKANAYI
                     TIPGLRGMPAKEISVKNLANIIQARMSEIMDFVTYHLKQVGLDNRMLNGGIVLTGGGS
                     QLKHLIQLTEYITGLNARIGYPTEHLAAGHIEELAKPTYSTCIGLILKGYSDYEHNRK
                     EFEKEYRKVDVPDNLRKAEAQPLTRPAHPTEEQLQQERVRNRKGVKDFWGKFKDGIID
                     LFKEEEDRTL"
     gene            2910..>3483
                     /locus_tag="VKQ52_18745"
     CDS             2910..>3483
                     /locus_tag="VKQ52_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HLZ89305.1"
                     /translation="MIHFDLPKEKSSIIKVIGVGGGGSNAVNHMFSQNIEGVNFIICN
                     TDAQAIAQSRVPNKIQLGPHLTQGLGAGANPDIGRQATQESLEEIKGILEVNTKMAFI
                     TAGMGGGTGTGGAPIISKICKDLGILTVGIITTPFSYEGKKRQLQAEEGIRVMKQYVD
                     TLLVISNDKLRHQFGNLKMKEAFAKADNVLA"
BASE COUNT          909 a          953 c          976 g          645 t
ORIGIN      
        1 aaggaattgt acccggagtt gaaatgcacg atcatcttcc agccgcatct ctttacgcgg
       61 acccgtgatt tcgcggatgg ctttgccgcc agtctcgaca gggccgatga ggtgatcctg
      121 ctgccgatct atcccgcacg ggaacaaccc atcgccggtg tgaccagtga actgatcgcc
      181 gggaagatga aaaaagcgaa aataacgatc gcggataaga aggaagtatt ggcgatgata
      241 aaggaaacgg ctgaacggcg cgaccaggcg caactgctga tcacggccgg cgcaggcgat
      301 attgatatgt tgatcgaacc cctccggcaa ctgctggaaa gcccattgtg aacgggaatt
      361 tcgtctaccc gggcactcgt tgtacccgtc tgacggtccg ctgggccgtt gggcgggtct
      421 caaaaaaaag accggaccgt gaggtctgta acaatatatg gccgtcaaat taaatatcag
      481 gaaagtagta tcagccgtct tctggatggt ggcgggcacg ggtgtgctgg tattactcat
      541 cgcggccata cgttaccgga acagcaatgt ctgcaagggc tttaagatcg ggatcaccgg
      601 cgggtcgtcc accggggacc acgcgctgtt catcgataaa aagggcattt ccgatatcct
      661 gaccgcggcc ggtgcgggaa agggccagaa caaacccatc cagtccttcg accttcgccg
      721 cctggaagcg gcgttgggaa ggaatgtatg gataaaggaa gcgcaattgt tctttgacaa
      781 taacggtatc ctgcaggtaa gggtcgtcga acgggagccg gtggcgcgcg tcttcacccg
      841 ggatgggaac agttgctata tcgacagcag cggggttcag ctgccgctgc cggacaagct
      901 cacggcgcgg ctgcccgtat tcaccgggta ccccggacag aagatcatgc tgcatggcgg
      961 cgacagtgtg ttgacgatgg agatcctgca tctcagtgat tttatccgcc gggatagttt
     1021 ctggacggcc cagatcgccc aggtggccat cctgcccgat aagggcttcg agctcgagcc
     1081 cgaggcggga gaccagcgca tcggctttgg cgacggccgc gacatcgacg aaaaattcca
     1141 ccggctctcg ctcttctata aagaagtcct gagccggacc ggccccggca aatacgatcg
     1201 tatcgacatc tcctatgccg ggcaggtcgt ggccacgagg cggggcactg ctcacgaccg
     1261 ttatgactcg acccagggaa tgaacaatat ccggcagatg atcaggtcgg cacagcaatt
     1321 gcaacccgac accatccgcc aacaaaatat ccgcccgctg gaacatgcta taatgacgga
     1381 gcaaaccccg gccgatctcg accttgtacc cgatgcagga gacagctcaa cccaaaaccg
     1441 taaaaaaaca actaaaaaaa aataaactaa actatgaatc aggaacaacc gattattgtc
     1501 ggactcgata tcgggacaac aaagatcgct gccatagccg gccgtaagaa cgagtacggc
     1561 aaactggaaa tactgggatt cggccgggcc aactccaatg gggtcaaaca cggccaggtg
     1621 ctgaacatcg acgagacgat aaaggccatc cagatggccc ttgacaattg ctatgcctcg
     1681 aaccccaatc tcgagatcag cgaggtctat gtcggcatcg ccggtcacca tataaagagc
     1741 cttcagaccc gcggtgatat cgtccggcag aacaccgacg aagagatcac ccgccgcgag
     1801 atcgatcagc ttgtcgccga ccagtacaag acctatatcc ccgccggcga ccagatcatc
     1861 gatgtgatcc cccaggagtt caccgtcgac aatttccaga acatccccaa ccccatcggg
     1921 tatggcgggg tgaaggtggg cgccaatttc catatcatca ccggtgacaa gaacgccatc
     1981 cgcaatatca accgggccgt ggagaaaagc aacctgcata ccaaggacct cgtactgcag
     2041 ccgcttgcct cggcggccgc cgtcatgggc caggaagacc tcgaagcagg cgtggccatc
     2101 gtcgacatcg ggggtggcac caccgatctt gccgtgttct atgaagggat attaaaacat
     2161 acggccgtta ttccttttgg cggggagaac atcaccaacg atataaagac ggggctgggt
     2221 gttctcaaga cccaggccga acagatgaag gtacagttcg ggtcggcctt gtccaatgag
     2281 gcaaaggcca acgcctatat caccatcccc gggctgcggg gcatgccggc aaaggagatc
     2341 agcgtgaaga acctggccaa tatcatccag gcccggatga gcgagatcat ggattttgtg
     2401 acctaccacc tcaaacaggt gggcctcgac aaccgcatgc tcaacggagg tatcgtcctc
     2461 accggcgggg gttcccagct gaaacacctg atccaattga cggaatacat caccgggctg
     2521 aatgcgcgta tcggctaccc gacggaacac ctggccgcgg gtcatatcga agagctggcc
     2581 aagcccacct attccacctg tatcggcctt atcctgaaag gatacagcga ctatgagcac
     2641 aaccgcaagg aattcgaaaa agaatatcgc aaggtcgacg ttcccgacaa tctcagaaaa
     2701 gccgaggcgc agcctctcac ccgcccggcc cacccgaccg aagaacagct ccagcaggag
     2761 cgggtccgca accggaaagg ggtaaaggat ttctggggaa aattcaagga cgggatcatc
     2821 gacctgttca aggaggaaga agaccgtacg ttataaatga atccgtgaag acccagtgaa
     2881 gagctatttc atttattaaa ttttttgtta tgatccattt tgatctaccg aaggaaaagt
     2941 catccattat caaggtcatt ggtgtgggtg gcggtggaag caatgcagta aaccatatgt
     3001 tcagtcagaa catagaaggt gtgaatttca tcatttgtaa taccgatgcc caggcgatag
     3061 cccagagccg tgtgcccaac aagatccagt tggggccgca tctgacccag ggtctgggag
     3121 ccggcgccaa cccggatatc ggcagacaag cgacgcagga gagtctggag gagatcaaag
     3181 gcatcctcga ggtgaacacg aaaatggcct ttatcacggc gggtatgggt ggcggcaccg
     3241 gcaccggcgg cgcgcccatc atctccaaga tctgtaaaga cctgggcatc cttaccgtgg
     3301 gtattatcac cactcctttc tcctatgaag gaaagaaacg gcagttgcag gccgaggaag
     3361 gcatccgggt gatgaagcag tatgtcgata ccctgctggt gatcagcaat gacaagctgc
     3421 ggcatcagtt cggcaacctg aaaatgaagg aagccttcgc caaggcggac aatgtactgg
     3481 cga
//
LOCUS       DATGFG010000990         6802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_118081, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000990 DATGFG010000000
VERSION     DATGFG010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118081"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..499
                     /locus_tag="VKQ52_18750"
     CDS             83..499
                     /locus_tag="VKQ52_18750"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF011116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HLZ89306.1"
                     /translation="MSNRTFTMIKPDAFAKGHSGAIIDKILKSGFRIVALKLTRLSKE
                     KAGEFYAVHKARPFYGELVDFMSSGPIIAAILEKDNAVADFRKLIGATDPAKADEGTI
                     RKLYAASVGENAIHGSDSDENAAIEGDFFFNAFERI"
     gene            complement(506..1414)
                     /locus_tag="VKQ52_18755"
     CDS             complement(506..1414)
                     /locus_tag="VKQ52_18755"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HLZ89307.1"
                     /translation="MMRKTLLLAAGMLTASLAFSQTPHTLLWRISGKTISHPSYLFGT
                     MHVLCAKDAALSDSLKGAIARCDEIYFEINLSDMAGMLNSMKYMRMNDSKKLSDLLKP
                     EEYARVKDYFARHASMLPFSLLERFKPMLISGLVEEQALDCETTDGMELMIMKEAKRG
                     KPVNGLETAEFQAGLFDSIPYETQAKELVNYIDSSEENKQMTRQLVDLYTHQDLDGID
                     ELSRKEDGGMSGYMDLLLYARNRKWAKNLDSLLGKRSLLIAVGAAHLPGTGGVIDLLR
                     KEGYTVEPVKNEVSNGQQQTAARSPL"
     gene            complement(1411..3339)
                     /locus_tag="VKQ52_18760"
     CDS             complement(1411..3339)
                     /locus_tag="VKQ52_18760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HLZ89308.1"
                     /translation="MAYRNQQEFIQALEKAGELVRIRGFVDPKLEIAEITDRISKSGD
                     GGKALLFENTGTDFPVLMNAYGSEKRMCMALGVAHLDDVTREIEQLFKLLSAPKEGLL
                     DKLKLLPKLGQFASWMPVVKSGRGECQEVVMSSPDIGRLPVITCWPKDGGPFITLPII
                     HTKDPNTGARNVGMYRMQIFGPDLTGMHWHKHKVSARHFAEYKKTGRRMPVAVALGGD
                     PAYAYSATAPLPENVDEYMLAGFLRKKKVELVKCITQPEIEIPADADFVIEGYVDPHE
                     EPIWEGPFGDHTGYYSLPDWYPRFHITAITHKKNPVYPATIVGIPPQEDAWLGKATER
                     IFLAPIKMTLVPEIVDMEMPVEGVFHNLVIAQIRKEYAGQGQKVMNAMWGAGQMMFNK
                     ILVIADEHTAIRDYERLARDVFRNLNPATDVYFSQGPMDVLDHSCSKLGFGGKMCIDG
                     TSKSEEETDSDMYQLQATAYEGLTEELLKSSRPEITDVNTTLLKRQIPCLVVAVRKER
                     PGQVRELHEALCGLDALEGVKMILYVEHTVDPGDLPVALWRFCNNLDPRRDHILTERP
                     SGTDAQRKFACLGLDGTRKTKELDNFQRDWPNIIVADDATIRAVDAKWNELGLGAFLP
                     SPSLKFKDQVYGEEAVVS"
     gene            complement(3429..4277)
                     /locus_tag="VKQ52_18765"
     CDS             complement(3429..4277)
                     /locus_tag="VKQ52_18765"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HLZ89309.1"
                     /translation="MKKILLVGMLSPLFTQAQWNVNLFGGFSNYIGDLQSKAYTTQQS
                     NGSFGAGLQYDVTGHFSLLSNLTYGRISAADGYAQKADLKARNLNFESVIGEWNLLAE
                     YNILDLQAHRLTPYVFVGLAVFHFNPYAHDTTGKKVYLRPLSTEGEGLAQYPGRKPYS
                     LTQLSIPFGGGLKFRISDRVTLAYEIGLRRTSTDYLDDVSSTYVSQAALLAAKGPEAV
                     EMAYRGNELKGGAGYPADGTVRGNPKRKDWYYMSGLRVTIAMNGLRMAEHRRHNGVLD
                     CPKKVY"
     gene            4594..4667
                     /locus_tag="VKQ52_18770"
     tRNA            4594..4667
                     /locus_tag="VKQ52_18770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4628..4630,aa:Pro,seq:ggg)
     gene            complement(6301..6453)
                     /locus_tag="VKQ52_18775"
     CDS             complement(6301..6453)
                     /locus_tag="VKQ52_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89310.1"
                     /translation="MEKLQPEKAAEMLHKKGMEVSVEQAALMLALLRMLAGIIVATYL
                     EQGRYP"
     gene            6522..>6802
                     /locus_tag="VKQ52_18780"
     CDS             6522..>6802
                     /locus_tag="VKQ52_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89311.1"
                     /translation="MFGKTNISSKLSVNCLNFTPLNDFVLICACLVGGAGKYSEEPYQ
                     NSSKLIKMDEKKTYQVTYKIYHNERIKKVSFHGKLVYPLYIRLTFDRRST"
BASE COUNT         1494 a         1873 c         1698 g         1737 t
ORIGIN      
        1 cttttcctta aatggccaaa accgccggct tccggtttgt gtcgaaatgc ctatcttgcg
       61 cggaaaattt agaaaggaat atatgagcaa tcgaaccttc accatgatca agccggatgc
      121 ttttgcaaaa gggcattcgg gcgccatcat cgacaagatc ctgaaaagcg gctttcgtat
      181 cgtggcactg aaactgaccc gcctgtccaa agaaaaggcc ggggaattct atgccgttca
      241 taaggcaaga cccttttatg gggaactcgt cgatttcatg agcagcggcc cgatcatcgc
      301 ggccatcctg gaaaaggaca atgccgttgc cgatttccgg aaactgatcg gcgccaccga
      361 ccccgccaaa gcggatgaag gcaccatccg gaagctctat gcggcctctg taggggagaa
      421 cgccatccat ggcagcgata gcgacgagaa tgccgccatc gagggggact tcttcttcaa
      481 tgccttcgag cgcatctgac ttttattata ggggtgaacg ggcagcggtc tgctgctgtc
      541 cgttcgatac ctcgttcttt accggttcca ccgtatatcc ttccttacgc agcaggtcga
      601 tgaccccgcc ggttccgggc aggtgcgccg cccccactgc tatcagcaac gatctcttcc
      661 cgagcaggct gtccagattc ttcgcccact tccggttgcg ggcatacaac aacaggtcca
      721 tatagccgga cattccccca tcctccttcc tgcttagttc atcgatgcca tccaggtcct
      781 ggtgggtgta aaggtccacg agttgccggg tcatctgttt gttctcctcg ctgctgtcga
      841 tatagttgac cagttccttt gcctgtgtct cgtaggggat gctgtcgaag agtccggcct
      901 ggaactccgc agtctcgagg ccgttcaccg gcttgccgcg tttggcctcc ttcatgatca
      961 tcagctccat cccgtccgtc gtctcgcagt ccagcgcctg ttcctcgaca agcccgctga
     1021 tcagcatggg cttgaaccgt tcgagcaggc tgaagggaag catcgacgca tgccgggcga
     1081 aataatcctt cacccgcgca tattcctcgg gcttcaagag gtcggacagc ttcttgctgt
     1141 cattcatccg catgtacttc atggaattga gcatacccgc catatcgcta aggttgatct
     1201 cgaaatagat ctcgtcgcac cgggcgatcg cgcccttcag gctgtcgctc agggcggcgt
     1261 ccttggcaca gaggacatgc atcgtgccaa acagatagga tggatgggaa atggtcttcc
     1321 ccgagatccg ccataacagc gtgtggggtg tttgggaaaa ggcaagggaa gccgtcagca
     1381 tccctgccgc aagcaaaagc gtttttctca tcatgacaca acggcctcct cgccgtatac
     1441 ctggtccttg aatttcaacg aaggggacgg aagaaaggct cccagtccca gctcattcca
     1501 tttggcgtcg acggcccgga tggtcgcatc gtcggcgacg atgatattgg gccagtcacg
     1561 ctgaaagtta tccaattcct ttgtcttacg cgtgccgtcg aggcccaggc aggcaaattt
     1621 tctttgtgcg tcggtcccgg agggccgctc cgtcaggata tggtcgcgcc gcggatcgag
     1681 attattacaa aaccgccata gcgccaccgg cagatcgcca gggtcgacgg tatgctccac
     1741 atacaggatc attttgacgc cttccagggc atccaggccg cacaatgcct cgtgcagctc
     1801 ccggacctgt cccggccgtt ccttccggac cgccacgaca agacagggta tctgccgctt
     1861 cagcagcgtg gtattgacat ccgtgatctc cggccggctg ctcttcagca gctcttccgt
     1921 gagcccctca taggccgtag cctgcaattg atacatgtcg ctgtccgtct cctcctccga
     1981 cttggaggtg ccgtcgatgc acatctttcc cccaaagccc aatttgctgc agctgtggtc
     2041 cagtacatcc atcggcccct gcgaaaaata tacgtccgtg gccgggttga ggttgcggaa
     2101 gacatcccgc gccagccgtt catagtcccg gatggccgtg tgctcgtcgg cgatgaccag
     2161 gatcttgttg aacatcatct gacccgcacc ccacatcgcg ttcatcacct tctgcccctg
     2221 tccggcatat tcctttcgga tctgcgcgat caccaggttg tgaaagaccc cttccacggg
     2281 catctccatg tccacgatct cgggaacaag cgtcattttt atcggggcga gaaagatccg
     2341 ttcggtcgcc ttgcccagcc aggcgtcttc ctgcggggga atgcccacga tcgtagcggg
     2401 atagacggga ttcttcttat gggtgatggc ggtgatatga aaacgggggt accagtcggg
     2461 cagcgaataa tagccggtat gatcgccaaa aggaccttcc cagatgggct cctcgtgcgg
     2521 gtcgacatag ccttcgatga caaaatcggc atcggcgggg atctcgatct cgggctgggt
     2581 gatacacttc accagttcga cctttttctt gcggagaaaa ccggctaaca tgtattcgtc
     2641 gacattctcc ggcagcggcg ccgtcgcgct gtaggcatag gccgggtcgc ccccgagggc
     2701 taccgccacc ggcatgcgcc gccccgtctt tttatactcg gcaaaatggc gggcgctgac
     2761 cttgtgcttg tgccagtgca tacctgtcag gtcaggcccg aagatctgca tccggtacat
     2821 ccccacattg cgggcgccgg tgttgggatc cttggtgtgg atgatgggga gggtgataaa
     2881 cgggccaccg tcctttggcc agcaggtgat caccggcagc cggccgatgt cggggctgct
     2941 catcacgacc tcctggcact cgcccctgcc gctcttgacg acgggcatcc aactcgcgaa
     3001 ctgtcctaat ttgggaagaa gttttaattt atccagcagg ccttccttgg gggcggaaag
     3061 cagcttgaac agctgctcga tctcccgggt aacatcatcg agatgggcta ccccaagggc
     3121 catacacatc cgtttctcgc tgccataggc gttcatcaga accgggaagt cggtgccggt
     3181 gttctcgaac aggagcgcct taccgccatc gccgcttttg ctgatccggt cggtgatctc
     3241 ggcgatctcg agcttcgggt cgacaaagcc gcggatacgc accaattccc ctgctttctc
     3301 caatgcctga atgaattcct gctggtttct gtatgccatg cagcaaaagt aaagcacaaa
     3361 ccgacctccg ccaaaacggt ccttgcaata ccacatggcc gggattggcc gggcttcacc
     3421 ccgcgccgtt aatacacttt cttcgggcag tccagcaccc cgttgtgccg gcgatgctca
     3481 gccatccgga ggccgttcat cgcgatcgta acgcgcaaac cggacatata ataccaatcc
     3541 ttacgcttgg ggttgcccct gacggtcccg tcggccgggt agcccgcccc acccttcagc
     3601 tcgttccccc ggtaggccat ttcgacggct tccggaccct tcgcggccag cagcgcggcc
     3661 tggctcacat aggtgctgct gacatcatcc aggtaatcgg tggacgtcct gcgcaacccg
     3721 atctcatagg ccagggtcac cctgtcggat atgcggaatt tgaggccacc gccaaaggga
     3781 atggacagct gtgtcagtga atacggcttt ctgccgggat actgcgcaag cccctcccct
     3841 tccgtgctca gcggccgcag gtatactttt ttgcccgtgg tatcatgagc atagggattg
     3901 aaatggaaca cggccagccc gacaaaaaca tacggcgtaa gccggtgcgc ctgcaggtcg
     3961 aggatattgt attcggcaag cagattccat tcgccgatga cactctcgaa attcaggttt
     4021 cgcgccttta gatccgcttt ttgggcatag ccgtccgccg cggagatgcg gccataagta
     4081 aggttggaaa gtagagagaa atgaccggtc acatcgtatt gcaggccggc cccgaaggag
     4141 ccattcgact gctgcgtcgt gtaggccttg gattgcaggt ctccgatgta attggagaag
     4201 ccgccgaata ggttcacgtt ccattgggct tgggtaaata gcggagataa catccccacc
     4261 agaaggattt tcttcattct agtttagaat ttgttgcgaa aaaggtattg ccgatggctt
     4321 aacgcagggt aagtggatta gatgggatta cgataaaggg gaaggagagg tttaacgatt
     4381 gtccgaagat tttccttggt agaagttttt acacagatga gatttgaagt tgataccgat
     4441 aacgcaattt ttaagccaaa tatttgcaaa cgaactgcca aaaaaaagtt aaagagaatt
     4501 ctgccggatt cgccgctttt ttccttacct ttgcaccccc accggaagtt gtccccgccc
     4561 aaaagcggct ggactccaac cggttgagaa tttcgggacg tagcgcagcc cggtagcgca
     4621 cttgcatggg gtgcaagggg tcgcaagttc gaatcttgtc gtcccgactt gacaggacaa
     4681 ataggaatta ttcccgtttg tcctgttttt attttcgctc aaacccttat ccagactgta
     4741 tattagccgg gccgcttcat tgacacgggt aattcgaagc cgatctccgt caaaagtcaa
     4801 tttttccggg tacatcgaac taattacctc ccttcttttc tctgtatctc cggttccata
     4861 gatgctatcc agccggaaca agttcttcaa ccccatattt aacaagtcgc taaagtctac
     4921 atgatcgttg ttgcatcccg ctaatttagc ttccaacttt tccagtttac cgttataatc
     4981 ggacttcatt tccctaaaat ctgccgggtc aatctgttta gaggctacca gatcccgcgc
     5041 gttcgataaa cggctttcca gttccctgat ctgctccaat atttgcacac ggtcgtcctg
     5101 tacatggctg gtctggtcgt accatgcctc ggttagagtt attttatata agtcctccac
     5161 ctccgggcgt gggatgaatt tcttcaattc atacacaaat tctttgttca cagtatcggc
     5221 attaaaccgg caggtacagc ccgtagtaca gtgataatag gtgtaatatt tagtatggcc
     5281 tttggaggta ctgccggtta atagcttgcc acatttcggg catatcagga atccacgtaa
     5341 cggtagattc tcattagtga ggattttagg tttgtaggtt gatcgcttac ggccatctaa
     5401 tacgtcctgc acctggtagt ataaaacttc ggatataatt ggctcatgta cccctttcac
     5461 gaattggcgt tcttcctctt tgtacttggg aatgaatatt tttccgcaat acaccgggtt
     5521 acggatggca aaccaaaaaa ggcttttggt ggcagtaaat cccttttctt ttgccatttt
     5581 atagacctgt tcggtattat ataccccctc cgctatctgc tcgaaagccc atcgtaaaac
     5641 tctggcctgt ggttcctggg gagcaatgta ttttttgccg gattcgtctg ttttattgat
     5701 ataaccgatg ggagccagtc ccatatatcg gccttccttc ctagcccgcc gcatcccgtg
     5761 gatcacattt aaggcccgcc ggtcgttctc tacttcagga gcagctaagt aaaacgcaag
     5821 catcattttg ttttccggaa tggaaagatc aaggggctgc tcgatggctt gcggttccac
     5881 ccccaatttt cgaagggtgt taatcatttg gtaggcatcc ccggcgttgc ggctgaaacg
     5941 atcccattta aggaaaagaa catgatctgt ttttcctttg tgtcggcgca gctctattaa
     6001 atactttttc cattccggcc ggttgaagct cttggcagaa tggtcctcat agatcacatg
     6061 gcggattgaa ataccgttaa tatcgcaata ccggcgcagc agttcttcct ggctgcgttg
     6121 ggagtaccct ttctcggcct gttcatccgt ggataccctc acatacaagt cagctatttt
     6181 cataatccag cattttaggc tttcaaaaat tagttatact tcaatttact aaaatcgggc
     6241 taaaaaacag cctgaaatac gaatgtttca ggctgttttg ttcgggagat gctccacttc
     6301 tcacggatac cggccttgtt ccagataagt ggcaacaatt ataccggcca acatccgcaa
     6361 gagcgccaac attagcgccg cctgttccac tgatacttcc attcccttct tgtggagcat
     6421 ttccgctgcc ttctccggtt gcaatttctc catttctcac agtttatctt tttcacaatt
     6481 gcgccctgat gaaagggcat tccctacaaa tataataata aatgtttggt aaaacaaata
     6541 tttcttccaa acttagtgtt aattgcctta actttactcc gttgaacgat tttgtactta
     6601 tttgtgcttg tttggtaggt ggtgccggaa aatattctga ggaaccatat caaaactctt
     6661 caaaattgat caaaatggat gagaaaaaaa cttatcaggt tacatacaag atctatcaca
     6721 atgagcggat caaaaaggtt tcttttcatg gtaaacttgt gtatccctta tatataaggc
     6781 tgacttttga ccggcggtca ac
//
LOCUS       DATGFG010000991         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_968641, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000991 DATGFG010000000
VERSION     DATGFG010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968641"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2506
                     /locus_tag="VKQ52_18785"
     CDS             <1..2506
                     /locus_tag="VKQ52_18785"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLZ89312.1"
                     /translation="MLDTAIMHTSQYTMGVDVADANNDGFPEIISMDMLPEDPYILKR
                     SEGEDAWDTYTQKLSYGYNHQYTRNNLQLNRRNGHFSEIGLYSGVAATDWSWSPLWVD
                     FDNDGLKDLFISNGIPRRLNDIDYINFIFDRELQQKTKGQRLDDNDISEADKFPQIKL
                     PSKFFKNEGKMVFSDQNARIQGGRNLYSNGAVYADLDNDGDLDIIVNNIDDKATIYRN
                     TANDHRRQAFLDIKLRGPAQNRNALGAKLILFSGRTIRTYEKYPVRGFLSSMELPLHV
                     GLQNTKVDSLFLVWPDNSFQRLTEIKDTGTLTLTYQEGLPVFDYSTITAFWKNPSAPM
                     EDITAQTNLRYDHKENDFHEFDREPLLPHMLSTEGPPLAVADIDKDGSQDVFIGSSKW
                     KKSAVFLQHHTGRFQRLDEPALDADSTYEDTDASWADINNDGKPDLLVASGGNEFYGN
                     DTMLTPRAYLNDGKGHLTRSPHAFPNIFVNASCIVPCDFNHDGFIDLFIGGRSVPWDY
                     GQVPRSYLLQNDGTGKFTDVTEQYAKGLAHIGFVTNAVWVDLDKDGRQDLLLSLEWGG
                     ILAFMHTPQGFTKKVITDKKGWWNFILPVDLNGDGNLDIIAGNLGLNSRLKASAEQPV
                     RLFYYDFDANGKKEQILTYYVHGQQIPFANKEELEKQMPGLKKRYLYAEDFAKATLED
                     LFTKDGLQKADTLSADYFSNAILINRGDGSFNLRAMPWEAQLAPFRDAVIVDANNDSL
                     PDILLVGNYYENNIQMGRYDADYGTLLLNEGRDSFSAVPINGLAIKGQTRHVREIGIN
                     GEKAYILARNDDSVKVIRFAPANKQANSQPNRKANK"
     gene            complement(2496..>2909)
                     /locus_tag="VKQ52_18790"
     CDS             complement(2496..>2909)
                     /locus_tag="VKQ52_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HLZ89313.1"
                     /translation="QTSLLVKEYPGTAVVYKAIEGLEGYIARHGGRRTGSPMVNITGV
                     AGGQYQLRVAIPTDTTLPESGDIRFRQLIRGKYLITDVRGGQAAVNSALDRINDYVSD
                     YRRTAMAIPFFSFVTDRSREADTTKWMTRIYYPIY"
BASE COUNT          757 a          919 c          680 g          553 t
ORIGIN      
        1 catgctcgat accgccatca tgcataccag ccagtacact atgggcgtcg atgtcgccga
       61 tgccaacaac gatggtttcc cggagatcat ttccatggac atgctccccg aagacccgta
      121 tatcctcaag cgaagcgaag gagaggacgc ctgggacacc tatacgcaga agctgagtta
      181 cggctataac catcaatata cccgtaataa cctccagctc aaccggcgca acggacattt
      241 cagcgagatc ggcctgtatt ccggcgtggc cgccacagac tggtcctggt cccctctgtg
      301 ggtcgatttt gacaatgatg gactcaaaga cctgttcatc tcgaacggca tccccaggag
      361 actgaacgac atcgactata tcaatttcat cttcgaccgg gaactgcaac aaaaaacaaa
      421 gggccagcgc ctcgacgata acgacatctc ggaagcagac aaattcccgc agatcaaatt
      481 gcccagtaag ttctttaaaa acgagggaaa gatggtcttc tccgatcaga acgcccgcat
      541 ccagggcggc cggaacctct attccaacgg cgccgtctat gccgacctcg ataacgacgg
      601 cgatctcgac atcatcgtga acaatatcga cgacaaggcc accatctaca ggaatacggc
      661 caacgaccac cggcgccagg ccttcctcga catcaaactc cggggacccg cccaaaaccg
      721 gaacgccctc ggagcaaaac tgatcctctt ttccggcagg accatcagaa cctacgaaaa
      781 atacccggtc agagggttcc tctccagcat ggagcttccc ctgcacgtcg gcctgcaaaa
      841 cacaaaggta gactccctgt tcctggtctg gcccgacaac agctttcagc gactgacaga
      901 aataaaagac accgggaccc tgacccttac ctaccaagag ggattaccgg tcttcgatta
      961 ctcgaccatc accgcgttct ggaagaaccc ttccgccccc atggaggata tcaccgccca
     1021 aaccaatctt cgatacgatc ataaggagaa cgattttcac gaattcgacc gcgaaccact
     1081 cctcccgcac atgctgtcta cggaagggcc tccactcgcc gtagccgata tcgataagga
     1141 tggatcacag gacgtgttca tcgggtcttc aaaatggaag aagagcgcgg tcttccttca
     1201 acaccatacc gggaggttcc aacgcctcga tgaacccgca ctggatgccg actccaccta
     1261 cgaagacacg gacgccagct gggccgatat aaataatgac ggcaaacccg acctgctggt
     1321 ggccagcggc ggcaatgagt tctatggcaa cgataccatg ctcacccccc gcgcttatct
     1381 caacgatggc aaaggacacc tcacccgatc gccccatgcc ttcccgaata tatttgtcaa
     1441 cgcatcctgc atcgtgccat gcgactttaa tcacgacggc tttatcgacc tgttcatcgg
     1501 tggcagatca gtaccctggg actatggtca ggtacccaga tcctacctcc tgcaaaatga
     1561 cggcaccggg aaattcaccg atgtgacgga acaatatgca aaaggtctcg cacacatcgg
     1621 cttcgtcacc aacgcagttt gggtcgatct ggataaggac ggccgccagg acctgctcct
     1681 ttccctcgaa tggggcggca tcctcgcgtt tatgcacacc ccgcaaggct ttaccaaaaa
     1741 ggtcatcact gacaaaaaag gctggtggaa tttcatcctc cccgtcgacc tgaacggaga
     1801 cggcaatctc gatatcatcg ccggcaatct cggcctgaac agccggctca aagcctccgc
     1861 cgaacaaccc gtccggctct tctactatga cttcgacgcc aatggcaaga aggaacagat
     1921 cctcacctat tatgtccacg gccagcagat ccccttcgcc aacaaggaag aactggaaaa
     1981 acaaatgccg ggcctcaaaa aaagatacct ctacgccgaa gacttcgcaa aagctacact
     2041 ggaagacctg ttcacaaagg acggcctgca aaaggccgat acgctcagcg ccgattattt
     2101 ctccaacgcg atcctgatca accgcggcga tggctccttc aacctccggg ccatgccctg
     2161 ggaagcacaa ctggccccct tccgcgatgc cgtcatcgtg gatgccaaca acgactccct
     2221 gccggacatc ctgctcgtcg gtaattacta tgagaacaat atccagatgg gaagatacga
     2281 tgccgactac ggcaccctgc tgctcaacga aggacgcgac tcgttttccg ccgtcccgat
     2341 caacggactc gccataaaag gacagaccag gcacgtgcgg gagatcggca tcaacggcga
     2401 aaaggcctac atcctggcca ggaacgatga cagcgtaaag gttatccggt tcgcccccgc
     2461 gaataaacag gcgaatagcc agccaaacag aaaggctaat aaataggata atagatccgc
     2521 gtcatccact tcgtcgtgtc cgcctccctg ctccggtccg tcacgaagga aaagaaaggt
     2581 atcgccatcg cagtccgccg gtaatcgctg acataatcgt ttattctgtc caacgccgaa
     2641 ttgacggccg cctgtcctcc ccgcacatcc gtgatcaggt actttcccct gatcaattga
     2701 cggaaacgga tatccccgga ctcaggcagg gtagtgtcgg tgggaatagc tacccgcagc
     2761 tgatactgcc cgccggcaac cccggtaata tttaccatcg ggctgcctgt acgccgccct
     2821 ccatgccggg cgatatagcc ttccagccct tcgatcgcct tatatactac agccgtaccg
     2881 ggatattcct tcaccagcag gctggtttg
//
LOCUS       DATGFG010000992         5901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1239140, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000992 DATGFG010000000
VERSION     DATGFG010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239140"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VKQ52_18795"
     CDS             <1..452
                     /locus_tag="VKQ52_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89314.1"
                     /translation="VPEPLAFFIHFGIKLLCSSAMIALFIVHVITGQLLRLLGYGFLS
                     FLLLVLCVAAPFYLLHLKGTLILAATIGTVLHLVVLLVLYHYRKREGRSEEKAFVVLS
                     LPYVLFYYLIIIGKEIDNAVFYVTATCCLPFFIRWYWAYSFRPRAIN"
     gene            467..2161
                     /locus_tag="VKQ52_18800"
     CDS             467..2161
                     /locus_tag="VKQ52_18800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ89315.1"
                     /translation="MIKGIICYSSKDAAIKAELLSHLRGLERIMDFRIWVDNELHAGD
                     RFEPEILSRILEAQVIILLISAYFNESDFCYTKEMKEAFRQADQKRLIIIPVLLREVN
                     TKGHPYEHLESLPKNPRNLSDWPSLDAGCVNVSAGIQRSIEHFVDELVASRKAEVWQL
                     ALEGHLERACMRLMDFAKEISRDPADIQQANSLLSAFNSEVRERKIAKDDFSLTTAVS
                     QGIFTLLNKLAGDSLPPEPPDRVSAPKAAFSKVYSHTREDGLIRDTMILGRNIGKRYR
                     RSNFELTIDHFQVRAGTITGIVGKNGSGKTTLLKIMAGVLAQDTGSLTYPFLDGGKPD
                     WAGIKRRIGYLPQELEKVRGKLLDSIWLNAILHGLNEKRIGQDIQYFLPRLGIDHLRE
                     ARWSELSGGFRLKFALASILICKPRVILLDEPLANLDIDSQQILLDDLRAFSTSLENP
                     IAIVLSSQQLREVQAVATHMVVLSKGNIAFSGMTSELGGDLGSNSFELGTPLSETQLR
                     KALTGLAVIRIEKRVSAYIITTPREITLRFITKYLVDNEVPFDLVKDESESSKRYF"
     gene            2167..3345
                     /locus_tag="VKQ52_18805"
     CDS             2167..3345
                     /locus_tag="VKQ52_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89316.1"
                     /translation="MPKIFPPAGSKLLRRYPHIWLTRLHVVLPATALLLVLSFFLGRR
                     LPLLTSHTERIWQSLLVLSAPCVFWIRWQYLHYQPYHRARDLYRFFLLNTMAGLLAGA
                     LLISTDWVMSRRIMDPHSRTAIYTDFRNLRIFAYYEDSLFAQGTRHPGARDPAGRPPI
                     YQDFTAKALAEDSSLHNHWPSYASVREQALKDTAKYDLPLYRPGRDSTMLRYYLNITL
                     FDRESLVGDIVPILTSYVEPSLLLYISPMFGLLLLHFVAGRSYRAFVSPLIAMWFLIL
                     LLLDLGGGPFGEGFTYMKTIDLLLYGLSLAGLFLLANGRRSSPGMRELSIQVFAYGAF
                     LLWNAVPFLLSDMDVKVSAGYVNGWSIAPALFYLSAFWLLFYYIRWYLSFTKRPRRLG
                     "
     gene            3429..3995
                     /locus_tag="VKQ52_18810"
     CDS             3429..3995
                     /locus_tag="VKQ52_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89317.1"
                     /translation="MGADEHMIEGAKALYEKLELFGNGSRAHELSAVVKQHALLGVGV
                     AAIPVPGLDIVALGVNTWTMYVRISEIVGVSFGEHALKAIASGVIANLASVIPGLALG
                     LGAEGLLKMIPGVGTLGGMAVGAVVNVGIMYASGKVYVKALEKLVNKGLPLTEENIAK
                     EAHDASKEKSFVTEAYKEGKTLAKNQKK"
     gene            complement(4005..4928)
                     /locus_tag="VKQ52_18815"
     CDS             complement(4005..4928)
                     /locus_tag="VKQ52_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89318.1"
                     /translation="MNWIEIKALHDLYIYGEVRLNETLSKSGELGFLADSYKVLHYSS
                     KTIQRLEGFTELYVKRYANNYAQYLAFLQERGFLKPQIRWEERDIQVLMKIKAGLDSG
                     ELTGLRDQIIAYEESQRGVSLMFFKNEKYLDTRPSLVEALKRLLKVSQFSNEKDQQYI
                     YRLECLQPRAIVLCENLDFLTKPTKPRAAGIELWYAGGKNVDKLSYADTRGLPVYYSC
                     DWDYDGLFIIYPLVKIKIPSIHLLNPDGPPRSIEKTEHNSRWNAARREDATFDHLFST
                     EQLDLIGQLVEANEWIIEESNDLLRMLAGTI"
     gene            complement(4897..>5901)
                     /locus_tag="VKQ52_18820"
     CDS             complement(4897..>5901)
                     /locus_tag="VKQ52_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89319.1"
                     /translation="KVQEMKDRHVADFNAYIRKYTIAIQQFEETSKGTSVGVNMEVSS
                     GRYAFDPLEEALLGTRIKTTDDIANALHDANQARLSMAEDIKSSMVRVFDTTLARYKK
                     FKDTVQKMNNFFKGKKISDQFFFKIEFAESKTLRIEFVEEIGEKMRRSASRGELAFDR
                     PVDEFIQEFFRKVAQLTEHIRIDKLLSPNTYFELSVSLTDENNNEISGSTGETYSAIA
                     LLGIARLSIAQSDKENRKGLRFIILEELGSLDNTNFNTFPSIAKEFDYQIVTMAPRPY
                     MSGLSTEWYAHHLIKGKVDEGINFHPSASYFQTKDHSGDLQTYLTRKNNELDRNKGAA
                     "
BASE COUNT         1350 a         1526 c         1450 g         1575 t
ORIGIN      
        1 ccgtacccga accacttgcc ttttttatcc actttgggat caagctcctt tgttcttcgg
       61 cgatgatcgc ccttttcatc gtgcatgtca tcaccggcca gttgcttcgt ctgctgggct
      121 atggatttct ttccttcctt ctgttggtcc tgtgcgtggc cgcaccgttc taccttcttc
      181 acctcaaagg aaccctaatc cttgctgcaa caatcggtac tgtcctacac ctcgttgttt
      241 tgctggttct ataccattac agaaaaaggg agggccggtc agaagaaaaa gcctttgtcg
      301 tcctgtcgct gccctatgtc ctgttctact acctgatcat aattgggaaa gagatcgata
      361 atgcggtgtt ctatgtcacc gccacatgct gtttaccttt ctttatccgg tggtactggg
      421 cctattcctt tcgaccacgg gcaatcaact gaaaatactt ttcaaattga taaagggaat
      481 tatttgttac agcagcaaag acgctgccat caaggcagag ctcctgagcc atcttcgcgg
      541 gctggaaagg atcatggatt tccgcatctg ggttgacaat gaattacatg ccggcgaccg
      601 cttcgaaccg gaaatccttt ccaggatatt ggaagcgcag gtgattattc ttttgatctc
      661 cgcttatttc aatgaatccg atttttgcta tacgaaggag atgaaggagg ccttcaggca
      721 ggccgatcaa aaacgcctca tcatcatacc ggtgctcctg cgggaagtga atacgaaggg
      781 gcatccttac gagcatcttg aaagcttgcc caaaaacccc cggaatctct cggactggcc
      841 ttccctggat gcggggtgcg tcaatgtctc cgccggcatc cagcgcagta tcgaacactt
      901 tgttgatgag ctggtcgctt cgaggaaagc ggaagtatgg caacttgccc tggaaggtca
      961 cctggagaga gcatgcatga gattgatgga tttcgcaaag gaaatctcga gggatccggc
     1021 ggatatacaa caagccaaca gccttctgtc ggccttcaat tccgaagtca gggaacgaaa
     1081 gatcgccaaa gacgatttct cccttacaac ggcggtaagc cagggtatct ttacgctttt
     1141 gaataaattg gccggtgatt ctcttcctcc ggagcctccc gacagggttt ccgcgccgaa
     1201 ggccgccttc agcaaggtct attcccatac ccgcgaagac ggcctcatcc gggatacgat
     1261 gatactgggc aggaatatcg gcaaacggta caggcgttcg aatttcgagt tgacgatcga
     1321 tcacttccag gtccgggcgg gaacgataac gggcatcgtg ggcaagaatg gcagtgggaa
     1381 gacgacgctg ctgaagatca tggcaggggt tttggcgcag gacaccggga gcctgaccta
     1441 tccgttcctc gatggcggca agcccgactg ggcgggtatc aaacgcagga tcggctatct
     1501 gccgcaggag ctggagaagg tgcgcggcaa actcctggat agtatatggc taaacgctat
     1561 tctgcatggg ttgaacgaaa aaaggatcgg gcaggatatc caatatttcc ttccccgtct
     1621 cggcatcgat catttgcgcg aggcgcgctg gtcggagctg tccggcggtt tccggctgaa
     1681 attcgccctt gcctccattt tgatctgcaa acccagggtt attttattgg acgagcccct
     1741 ggccaacctg gatatcgatt cacagcagat attgctcgac gatctcaggg ctttttccac
     1801 cagtctcgaa aaccccatcg ccatcgtatt gagcagccag cagcttaggg aagtacaggc
     1861 cgtagccacc catatggtgg tgctgtcgaa ggggaatatc gctttttccg ggatgacctc
     1921 cgagttgggc ggagacctgg gttccaactc cttcgaactg gggactccgc tttcggaaac
     1981 ccaattgagg aaggcgctca ccggcctggc agtcatcagg attgaaaaaa gggtatctgc
     2041 ctatattatc acgactccca gggagatcac cctccggttt atcacgaagt acctggtgga
     2101 taatgaggtt cccttcgatc tggtaaagga tgaaagcgag tcgtccaaac gctattttta
     2161 gaatctatgc caaagatatt ccctccggcc ggctcaaagc tcctgcgtcg atatccccat
     2221 atctggctca cccgcctgca cgtggtattg ccggccactg ccttattgct tgtcctgtcc
     2281 ttctttctgg gccggcgcct ccccctgctg acaagccata cggagcggat ttggcaatcc
     2341 ctgctggtcc tgtcggcgcc ctgtgttttc tggatcagat ggcagtattt gcattatcaa
     2401 ccctatcacc gggctcgtga tctataccgc ttttttctgt tgaacacaat ggcaggcctt
     2461 ctggccgggg cgttactgat ctccaccgat tgggtgatga gccgcaggat catggacccg
     2521 catagcagaa cggccattta taccgacttt cgcaacctca ggatcttcgc ctattatgaa
     2581 gactcccttt ttgcccaggg aacaagacat cccggtgcaa gagatccggc cggccggcca
     2641 cccatctatc aggactttac cgcgaaagcg ctggcagaag acagcagcct tcacaaccat
     2701 tggcccagct atgcatctgt cagggagcag gcgttaaagg acactgcaaa atacgatctc
     2761 cccttgtaca ggcccggcag ggacagcact atgctgcggt actatttaaa cataacttta
     2821 tttgaccggg agtctctcgt gggagatatc gtccccattt tgaccagcta tgttgaacct
     2881 tcccttcttc tctatatttc cccgatgttt ggattgttac tccttcattt cgttgccgga
     2941 cgcagttacc gggccttcgt atccccgctt atcgcgatgt ggtttttgat cctgctcttg
     3001 cttgacctgg ggggcggccc tttcggcgag ggttttacct atatgaaaac gatcgacctc
     3061 ctcctctatg ggctgagcct ggccggactc ttcctgctcg caaacgggcg ccgaagctcc
     3121 cccgggatgc gggagctgtc tatccaggta ttcgcttatg gggctttcct gctttggaac
     3181 gcggtccctt tccttttatc agatatggac gttaaagtct ctgccgggta tgtcaacggg
     3241 tggagtatag ccccggcgtt attttatctt tccgcgttct ggctactctt ctattatatc
     3301 cggtggtatt tgtcctttac caagaggccg cgccgcctcg gctaaaaaca ggtgttttcc
     3361 ccgttgcgca cctctttgcg tagccttatc ttggtgataa atttttaaac cagtaaaact
     3421 tatccaacat gggggcagat gaacatatga tcgaaggcgc caaggcgtta tatgagaagc
     3481 tggaattgtt tggcaatggt tcgagggcgc atgaattgtc ggccgtggtc aaacaacatg
     3541 cgctgctggg tgtgggtgtt gcggcgattc cggtacctgg cctcgacatt gtcgcgcttg
     3601 gcgtcaatac ctggacgatg tatgtacgca tcagtgaaat tgtaggggtt tcatttgggg
     3661 agcatgcgct caaggcaatc gccagtgggg tcatcgccaa cctggcctcc gtgattcccg
     3721 gattagccct gggattgggc gccgagggtc ttctcaagat gattcccggc gtgggcaccc
     3781 ttggcggcat ggcggtgggg gccgtcgtaa atgtagggat aatgtatgcc tccggaaagg
     3841 tatatgtaaa ggctttggag aaattggtca acaaggggct gccgttaacg gaagagaaca
     3901 ttgcaaaaga ggcccatgat gcgtcgaagg aaaagtcctt tgtgacggaa gcctacaaag
     3961 aaggaaaaac gctggcaaag aatcaaaaga agtgaacagg cgcttcagat cgtccctgcg
     4021 agcattctca ataaatcgtt ggattcttcg atgatccatt cattggcctc taccagttgc
     4081 cctataaggt ctagttgctc agtagagaac aaatggtcga atgtcgcgtc ctctcgtctt
     4141 gcagcattcc aacggctgtt gtgctccgtt ttttcaatac tcctgggggg accatccggg
     4201 tttaacaggt gaatggaagg gatctttatt ttaaccaacg ggtaaattat aaacagccca
     4261 tcataatccc agtcacaaga gtaatagacc ggtaatccgc gcgtatcagc ataagatagt
     4321 ttgtctacgt tctttccgcc ggcgtaccac aactcgatcc ccgctgccct tggtttggtt
     4381 ggcttggtaa gaaagtccag gttttcacac agaacaatcg ccctgggctg aagacattcc
     4441 aacctgtaaa tatattgctg gtccttttca ttggaaaact gagacacttt cagcagccgc
     4501 ttaagcgctt ctacaaggga cggcctggta tcgaggtatt tctcattttt gaagaacatc
     4561 aacgagacgc cgcgttggga ttcctcatag gcgataatct gatcccgcaa tccggtcagt
     4621 tcgccggaat ccagtccagc ctttatcttc attaaaacct ggatatcccg ctcctcccac
     4681 cggatctgcg gcttaaggaa gccccgctcc tgcaaaaatg ccaaatactg cgcatagttg
     4741 tttgcatacc tctttacata caattccgta aaaccttcca gtcgctgaat cgtcttgctc
     4801 gaataatgaa gtactttata agagtccgcc aaaaagccca attcaccgct ttttgaaagc
     4861 gtttcattca gacgaacctc accatagata tacaggtcat gcagcgcctt tatttctatc
     4921 caattcattg ttctttctgg ttaaataggt ttgcagatct ccgctatgat cctttgtctg
     4981 aaaataggac gccgatgggt gaaaattgat gccttcatct actttcccct ttatcaaatg
     5041 atgtgcatac cattctgtgg aaaggccact catatatgga cgtggcgcca tggtaacgat
     5101 ctgataatca aactcctttg caatagaggg gaaggtattg aaattggtgt tatcaaggct
     5161 gcccagttct tccagaataa taaagcgaag cccctttcta ttctctttat ccgattgggc
     5221 aatggaaagc cgggcaatcc cgagcaaagc gatggcagaa taggtttcac ccgtacttcc
     5281 ggagatctca ttattgttct catccgtcag gcttacgcta agttcaaaat aagtattcgg
     5341 gctcaacaat ttgtcgatcc tgatgtgttc ggtgagctga gccacctttc taaaaaactc
     5401 ctggatgaat tcatccacgg gtctgtcaaa agcgagctcg ccccggctcg ccgaccggcg
     5461 catcttttcg ccaatttctt cgacaaactc tatcctgagc gtcttacttt cggcaaactc
     5521 tattttgaag aagaattgat cgctgatctt cttcccttta aagaaattat tcattttttg
     5581 caccgtatcc ttgaatttct tgtatctcgc gagcgtggta tcaaaaaccc gcaccatgct
     5641 ggatttgata tcttcagcca tgctcaatcg ggcttgattg gcatcgtgca gggcattggc
     5701 aatgtcatcc gtggttttga tccttgttcc taataaggcc tcttccaaag gatcaaaggc
     5761 atagcgacca ctgctaacct ccatatttac cccaacgctg gtgcccttgc tggtttcctc
     5821 gaactgttgg atggcgatag tatattttcg gatatatgca ttaaagtcgg ccacatgtct
     5881 atctttcatt tcctggacct t
//
LOCUS       DATGFG010000993         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_412458, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000993 DATGFG010000000
VERSION     DATGFG010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412458"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(498..1853)
                     /locus_tag="VKQ52_18825"
     CDS             complement(498..1853)
                     /locus_tag="VKQ52_18825"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLZ89320.1"
                     /translation="MSTGIVLIIDDEEKLRTLLARIIRLEGLTVLEAETIKAGNRLLD
                     KEDIDVVLCDVKLPDGNGVDFVRDLKKRHPSVETILLTAYGNIADGVAAMKNGAFDYI
                     TKGDDNDKIIPLLSRALEKVQLQKRVDQLEQQVGKRYTFDGILGHSPLIMEAIALARK
                     VAPTEATVLLLGETGTGKEVFAQAIHHGSKRATKPFIALNCSSFSKELLESELFGHKA
                     GSFTSASRDKKGLIEEANLGTLFLDEIGEMHIDLQAKLLRVLETSEFIKVGDTRPTRV
                     NVRIIAATNRELLQEVTDGGFRDDLYYRLNVFTIFLPSLRDRKKDIPLIANFYLRFFS
                     GKGNKRIDSMSKEFLDRLQQHEWKGNIRELKNIIERAVILSDGPQLTVDNLPSELQVS
                     GLRSSGVLSAFDLASVEKLHIQRVLNHTRGNKTEAARLLNIGLTTLYRKIEEYRLSTD
                     A"
     gene            2007..2093
                     /locus_tag="VKQ52_18830"
     tRNA            2007..2093
                     /locus_tag="VKQ52_18830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2041..2043,aa:Ser,seq:cga)
BASE COUNT          497 a          597 c          668 g          538 t
ORIGIN      
        1 aatttctccg gttttaccag tacatagcag aggtaaatga ataccaggat ggaaacgatg
       61 aataataagg tcataccagg atggacggga agggttttgc cggagtggcc cgtctcccgt
      121 cgccaatttt atgacgggcc ccggccggcg gacctgtgga ggccctaaat cttgtcaaaa
      181 aagtcgatcg ctttataaaa aagggcaaag cagataagcc ctgccagcac aagaagcacg
      241 gtcagcatgt ttttttgcgg gtgtaaggcc aaaacgaatg cctaaatgcc gggatcttcc
      301 ggcttaccag tcaaacacca cacaggcttg ggtttcagcg cgtccccgat ccggccctgg
      361 ccgtttggcg gcagcgcccc cctttcaaaa tggaagactt ttagccgaaa tgggtggccg
      421 gattggaggg gggccaggac agggagagcg gcccgcaagg gacatgccgg taggggcggc
      481 cggcaggaca tcggcggcta ggcgtcggtg ctgaggcgat actcctcgat cttccggtag
      541 agtgtcgtca gtccgatatt gaggaggcgg gcggcttcgg tcttgttgcc gcgggtatgg
      601 ttcagtacac gctggatatg cagtttctct acactggcga ggtcgaaagc ggaaagcacc
      661 ccggaggacc ggagaccgga gacctgcaat tcgctgggca gattgtcgac ggtgagctgg
      721 ggcccgtcgg agagaatgac ggccctttcg ataatattct tcagctcccg gatattcccc
      781 ttccattcgt gttgctggag acgatcgagg aattccttgc tcatgctgtc gatacgcttg
      841 ttccccttgc cggagaagaa gcggagatag aaattggcaa tgagggggat gtctttcttg
      901 cgatcgcgca gggaaggcag gaagatggtg aatacgttga ggcggtagta caggtcgtcg
      961 cgaaacccgc cgtccgtgac ttcctgcagg agctcgcggt tggtagcggc gatgatccga
     1021 acattgaccc gggtggggcg ggtatcgcca actttgatga attcgctggt ttccagtacg
     1081 cgcagaagct ttgcctggag atcgatatgc atctcgccga tctcatccag gaaaagcgta
     1141 cccaggttgg cttcttcgat aagtcctttt ttgtcgcggc tggcgctggt aaaagagcct
     1201 gctttgtggc caaagagttc gctttcgagc agttccttgc tgaaggagct gcagttaagc
     1261 gcgataaagg gtttggtcgc ccgcttgctg ccatgatgga tggcctgggc aaagacctct
     1321 tttcctgtac cggtctctcc cagcagcagg acggtcgctt cggtgggggc caccttccgg
     1381 gccagggcga tggcttccat tatcaggggt gaatggccca ggatgccatc gaaggtgtat
     1441 cgcttcccga cctgttgttc cagctggtcc acccgtttct gcaactgcac cttctcgagg
     1501 gcccgggata aaagggggat gatcttatcg ttatcgtcgc ccttggtgat atagtcgaag
     1561 gccccgttct tcatggccgc taccccgtcg gcgatattgc cataggccgt cagcaggatg
     1621 gtctcgacgg aggggtggcg tttcttcaga tcccggacaa agtcgacccc attgccatcc
     1681 ggcagcttga catcgcagag cacaacatcg atatcctcct tgtccagcag cctgttgccc
     1741 gcctttattg tttccgcctc caggacggta agcccttcca gccggatgat cctggccagc
     1801 agggtacgca atttttcctc gtcgtcgatg atcagaacaa tacctgttga catttccatg
     1861 cgggattgag gggcaaaact aaggattgcg gatgaaccga ccgccggccg gccgggtttt
     1921 ccaccaaacc cttcacaaat gcaaaaaatt gctccaaatc cattattttt gcaccccgac
     1981 cgacaatcct cattgtcaat cccttcggag aggtggcaga gcggtcgatt gtggcggtct
     2041 cgaaaaccgt tgtgcgggca accgtaccga gggttcgaat ccctccctct ccgctaatga
     2101 cgttttaaac gatgttgaaa aagctcacaa gatattgatt ctgtgggctt ttttcatttc
     2161 ggaatacaaa tcgattcaaa agaattcaaa gctttaggtg gcattttagg tagcccaggt
     2221 tttcgacaaa ttttgggaca cctgaacggc cgcctccgcc acgacaagga ttctttgcct
     2281 ggaatttttg gtgacgaaca
//
LOCUS       DATGFG010000994         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_623343, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000994 DATGFG010000000
VERSION     DATGFG010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623343"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VKQ52_18835"
     CDS             <1..664
                     /locus_tag="VKQ52_18835"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HLZ89321.1"
                     /translation="ANHPEMEYVFGKAHSETVEYEHKIRIGKRSGTVRLTFSNTASRA
                     PSYKEGMEALKAGDTNLLNVISGNAERTTFGGHKTGFGLSADQRLTDDIGAFVRAGWN
                     DGKYVTWAFTEIDQTLSGGLSFKGTHWKRKDDVIGIAGVINGLSAAHRDFLKAGGYGF
                     IIGDGNLNYGHEAIVETYYNARLFPNVWATFDYQFVHNPGYNKDRSGPVHVFAVRGHI
                     EF"
     gene            680..1171
                     /locus_tag="VKQ52_18840"
     CDS             680..1171
                     /locus_tag="VKQ52_18840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ89322.1"
                     /translation="MRITPLFPLHWPAVKAIYEEGLATGNASFQTGAPSWAEWDNAHL
                     AHSRMVALDGDTVIGWAALTPVSGRCVYAGVAEVSVYIASGSRGQGVGKALLRALISE
                     SEANGIWTLQAGIFPENTASICLHEGAGFRQIGRRERIGRMGDRWRDTVLLERRSQVV
                     GVD"
     gene            1227..1820
                     /locus_tag="VKQ52_18845"
     CDS             1227..1820
                     /locus_tag="VKQ52_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLZ89323.1"
                     /translation="MDIHLSVLERRVGTFVIKAYIAEDLPQYPYHNLVHTQSVVTRVR
                     EIAGYYGLGSTDHFVLVAAAWFHDIGHLCGEPEGYEDRSVRIMEQQLADIPEGIRSGI
                     AGCIIASRSPGYPLTTNEKILCDANTYHYGTPLFTDVDGLMRREMEMRGGMTIPDWHR
                     RRVELLQRHRFFTEYCQQLLEPGKQRNIAWLEKKGGI"
     gene            1885..2217
                     /locus_tag="VKQ52_18850"
     CDS             1885..2217
                     /locus_tag="VKQ52_18850"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ89324.1"
                     /translation="MGLTKSEIFTDKQNKLAAMMKALAHPARIAIIQYLIKANACING
                     DLVEELGLAQPTISQHLKELKNAGLIQGTIEGTSVCYCIEPKTWALYQKEIGALFAAY
                     KGADNCCG"
     gene            2291..>2687
                     /locus_tag="VKQ52_18855"
     CDS             2291..>2687
                     /locus_tag="VKQ52_18855"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLZ89325.1"
                     /translation="METTEQIKDMVREKYSAIALQDKEDNAASCCGSGCCSIETANIM
                     TDDYSTLEGYNPDADLGLGCGLPTQFARIKKGDTVIDLGSGAGNDCFIARKETGETGR
                     VIGIDFTPAMIEKARANVDKLGYNNVEFRQ"
BASE COUNT          629 a          700 c          807 g          551 t
ORIGIN      
        1 tgccaatcat cccgagatgg aatatgtttt tggcaaggcg cattcggaga ccgtggaata
       61 cgagcacaag atcaggatcg gcaaaaggtc ggggacggtc cggctgacgt tcagcaatac
      121 cgcttcccgg gctccttcgt acaaggaggg tatggaggcg ctgaaggcgg gggacacaaa
      181 cctgttgaac gtgatctccg gcaacgcgga aaggacgacg tttggcgggc ataagacggg
      241 gtttggcctt agcgccgacc agcgacttac cgatgatatc ggcgcctttg tccgggccgg
      301 gtggaacgac ggtaaatatg ttacctgggc ttttacagag atcgatcaga cgttgagcgg
      361 cgggctatct tttaagggca cgcattggaa aagaaaggat gatgtgattg ggatcgccgg
      421 ggtgatcaat gggctctccg ctgcccaccg ggacttcctc aaggccggcg gttatggctt
      481 tatcatcggc gacggcaacc tgaactatgg gcacgaggcc atcgtcgaga cctactacaa
      541 cgcgaggcta tttcccaatg tctgggccac cttcgactac cagttcgttc ataatcccgg
      601 ctataataaa gaccggagcg gacccgtgca tgtgtttgcc gtccgggggc atatcgaatt
      661 ttgatcgtat cttcgacggg tgaggatcac cccattattt cccttgcatt ggccggcggt
      721 gaaggccatc tacgaagagg gtctggcgac cgggaatgcc agttttcaga ccggcgcccc
      781 ttcctgggcg gagtgggaca acgctcattt agcacatagc cggatggtcg cactcgatgg
      841 cgacacggtc atcgggtggg ccgcactcac gcccgtgtcg ggacgttgcg tatatgcggg
      901 ggttgccgag gtgagtgtct atatcgcgtc agggagccgt gggcagggag tgggaaaggc
      961 gctgttacgg gcattgataa gcgaaagtga ggccaatggc atctggacgc tgcaggccgg
     1021 catcttcccc gaaaacaccg ccagcatttg cctgcatgaa ggcgccgggt tccgccagat
     1081 cggaagacgg gaaaggatcg gcaggatggg agaccgctgg cgcgatacgg tgttgctgga
     1141 aagaagaagc caggtggtgg gggtggattg agaaatccag aatatcttca gtttaccggc
     1201 gcatattgat gccttagcag caacccatgg atatacacct ctccgtctta gaacggcgtg
     1261 tcggaacctt cgtcatcaag gcgtatatcg ccgaggatct tccgcaatac ccgtatcaca
     1321 atctcgtcca tacgcagagt gtcgtcaccc gggtgcggga gatagccggc tattacgggc
     1381 tgggaagcac cgaccatttt gtgctggtgg cggcggcctg gttccatgat atcggtcatc
     1441 tctgtggcga accggagggt tatgaagacc ggagtgtgcg gatcatggag cagcagcttg
     1501 cggatatccc ggaggggata cgatcgggga tcgccggctg tatcatcgcg agccggtcac
     1561 cgggctaccc gcttaccacc aacgagaaga tcctttgtga tgccaatacc taccattatg
     1621 gtacgccgct ttttaccgat gtcgatggcc tgatgcggcg tgagatggag atgcgtggcg
     1681 gaatgaccat cccggactgg caccggcgcc gcgtggaact gttgcagcgg catcgctttt
     1741 ttacggaata ctgccagcag ctgctggagc cgggcaaaca acggaatata gcatggctgg
     1801 aaaagaaagg ggggatataa tttttttgca gaaagatgct tttttccgca ttttatcgta
     1861 atattgcaat gaaacgatca atgaatgggg ctgactaaat ccgaaatatt caccgacaag
     1921 cagaacaagc ttgccgccat gatgaaggcc ctggcccatc ctgcgcggat cgccattatt
     1981 caatacctga taaaggccaa tgcctgtatc aacggcgacc tggtcgaaga gttggggctg
     2041 gcgcagccaa ccatatccca gcatctgaag gagctgaaga atgccggcct gatccagggg
     2101 accatcgaag gcactagtgt ttgctattgc atcgagccga agacctgggc cctgtatcaa
     2161 aaagagatcg gcgccctgtt cgccgcctac aaaggcgcag acaactgctg cggataaccg
     2221 gctgcggata atcccgtcgc attttttttg atccaaccca tcgtaatatc gcaatattaa
     2281 aatatatagt atggaaacga cagaacaaat aaaagacatg gtgcgcgaaa aatacagcgc
     2341 catcgccttg caggacaagg aagacaatgc cgcctcctgc tgcggctccg gctgctgctc
     2401 catcgaaacg gccaatatca tgaccgacga ctacagcacc ctcgaaggct ataatcccga
     2461 cgccgacctg ggcctgggtt gcggccttcc cacccaattc gcgcggatca aaaagggcga
     2521 caccgttatc gacctgggct ccggcgcggg gaatgactgc tttatcgccc ggaaggagac
     2581 cggcgagacg ggccgcgtca tcggcatcga cttcactccc gcgatgatcg agaaggcacg
     2641 cgccaacgtc gataaacttg gctataataa cgtcgagttc cgccagg
//
LOCUS       DATGFG010000995         5231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1043063, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000995 DATGFG010000000
VERSION     DATGFG010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043063"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VKQ52_18860"
     CDS             complement(<1..338)
                     /locus_tag="VKQ52_18860"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ89326.1"
                     /translation="MKALVTGSAGHLGEAMVRWLQAANHPVLGLDILDSPFTHRVGTI
                     TDRDFVRGCLEGVDTVYHTATLHKPHVATHTPQQFIDTNISGTLNLLQEAVAAGVKRF
                     IFTSTTSVFG"
     gene            512..1312
                     /locus_tag="VKQ52_18865"
     CDS             512..1312
                     /locus_tag="VKQ52_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89327.1"
                     /translation="MKKLTEMGPLFYAIAVAGFGVNQLVTQNFLTGLLPVPASLPLRA
                     FWMVLSSVVFLIAAVGMVFLGRKYLAAALAGTQFFLFFLLLHLPKLLGDIYNPVVWTP
                     PFETLMLGSGGFMIAAYQINTGAGDPKWDKPLNIMGLISQYLFAVGLVVFAVLHIKYN
                     DYIQTLIPAWMPGHLFLSYVVIAAFLLSALSFFTGLKLPLASGLLGIMFLLWVILLHA
                     PRAIGKVNVEPEWTSLFVALAVCGSAFTICRQTQVWERRRVQRGALAN"
     gene            complement(1401..2255)
                     /locus_tag="VKQ52_18870"
     CDS             complement(1401..2255)
                     /locus_tag="VKQ52_18870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ89328.1"
                     /translation="MNNTTIGWIGLGNMGTPLSQRLLGAGYSVTVYNRSKEKEAAFRA
                     AGAGIAPTPAHLIKATDILFLMVSDDQAIRDVFTGDNGLLSAKTSGKLLINMSTVSPA
                     ISREFAAACAQQGNEYLDAPVSGSVKQAQEGQLVIMAGGSGTAYEKANPILQHLGKLV
                     LHLGGPGSGNTAKLAINILLGFHAQGLAEAAIFAEHHGVKTADFMAILNNSALGNVFG
                     KIKGDAIISDNYKAAFALKLIAKDLRLARAEGWDTPMAEVTWQSFQQAEARFGEEDII
                     AIKKQLDH"
     gene            2429..2809
                     /locus_tag="VKQ52_18875"
     CDS             2429..2809
                     /locus_tag="VKQ52_18875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ89329.1"
                     /translation="MFRTNVKKPVTRVLVIEDEGDMCLLLELLLDGEGMVVDHVRSLS
                     GAREFLEKEQPSLIVLDNRLPDGLGIDFIGYLKAQYPAIKILMISGVDIEAKDAALEI
                     GADTFLSKPFTQSQLTSSVSSLLN"
     gene            3128..3562
                     /locus_tag="VKQ52_18880"
     CDS             3128..3562
                     /locus_tag="VKQ52_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89330.1"
                     /translation="MKPTKSSISKIEVIIEKNDGVLWGRVENMGDHLLTPYGESTNEV
                     IKNLKELVRDYLAHEGKKDKFWNRVDINKVDFEFSYDLQAFFAEHDYLKISSVAEYAG
                     LNAGLLRQYASGVKFPSQDQAKKIEDAIHYIGEELREISIYA"
     gene            complement(3659..>5231)
                     /locus_tag="VKQ52_18885"
     CDS             complement(3659..>5231)
                     /locus_tag="VKQ52_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89331.1"
                     /translation="VNGPNFGFAHSAGDSFGAVLCDATSHAPDRYVTFPWVNIGRRHD
                     RAVAAGWGWGGVMDTGGYNSEQILCTTHFRIYRAIGGDSTEVAMRKFAGRYVPYLILR
                     TVGMLTQPTNPPNSMAYATDLMSAELGNWTTADQVGGVYWKVIRWAFEKQGLFQPLGA
                     PTPVWTEGAPPPVDVFIEDGRHGEYQYGPANQYPYLQKWWETTEIWNRHAADGHAEHQ
                     TPIVGKINHAYVRIRNRGTQAASGVVVHGYHCRPGAGLVWPDDFQPMKTTSFTVPGVI
                     PPGGEVLAGPFYWKPKYKGHDGMYMSVSAFGDRANDDALTLLPAAAGPSPLWRLVPCD
                     NNIGLRAVIPVPGGGHRRALVAAFRKRRFWASNPTAKTGKVEVRAILPAFLATRGWAI
                     QLANPGSGSFTLGPRDSRMIRPRLIGGQNFTAAEVAAAGEVAIRLVVLVEGLVVGGLT
                     YVLDPALKEPALEWEHEIKEEFEEIEEIEEEEEEEEHEHHGHHEHHEFHGHHDHDDHD
                     HDKRPRRIRLDIDLD"
BASE COUNT         1036 a         1226 c         1732 g         1237 t
ORIGIN      
        1 tcgccgaaga cgctggtggt gctggtgaag atgaagcgtt tgacacctgc ggcgactgcc
       61 tcttgcagga ggttgagcgt accggaaata ttggtgtcga tgaattgctg tggggtatgg
      121 gtcgccacat ggggtttgtg gagggtggcg gtatggtaga cggtgtccac gccttcgagg
      181 catccgcgga cgaagtcgcg gtcggtgatg gtgccgacgc ggtgcgtgaa gggagagtcg
      241 aggatatcga gcccgaggac gggatggtta gcagcttgca gccatcggac catggcctca
      301 ccgagatggc cggcactgcc ggtaacgaga gctttcatcg attggtgttc tttgttcctg
      361 agcgatgaag ataagaatat ttcgaggaag atgggggcat agagggttgt acgatcttgt
      421 acggagatgt acaagaggtg attcgggggt ttgtgctttt tggctgaatt ttatcctgcc
      481 ccgcgttgtt gcgtggtata aagacaggat tatgaagaaa ctaactgaaa tgggacccct
      541 cttttatgcg atcgcggtcg ccggattcgg tgtcaatcaa ttggtgacgc agaatttttt
      601 gacggggttg ctccccgtgc cggcgagctt gccattgcgg gccttctgga tggtcctgag
      661 cagtgtagta ttcctgatcg cggcggtggg aatggtgttc ctggggcgga agtacctggc
      721 ggcggcgctg gcgggcacgc aattctttct tttctttctg cttctccatt tgccgaagtt
      781 gctgggggat atttacaacc ccgttgtctg gacgccgccg tttgagacgt tgatgctggg
      841 gagcgggggt tttatgatcg cagcctacca gatcaacacc ggggcggggg atcccaagtg
      901 ggacaagccg ttgaatatta tggggcttat cagccagtac ctctttgcgg tggggcttgt
      961 cgtttttgcg gtgcttcata taaaatacaa tgattatata cagacgctga tcccagcctg
     1021 gatgccgggg catttgtttt tgtcctatgt cgtgatcgcg gcctttctat tgtcagcttt
     1081 gagtttcttc accgggctga agcttccact ggcgtcgggg ttgctgggaa ttatgttcct
     1141 gctgtgggta atactactgc atgcaccccg cgccatcggg aaggtgaatg tcgagccgga
     1201 atggacgagt ctttttgtcg cgttggcggt atgcggatcg gcatttacta tttgccggca
     1261 aacacaggta tgggagaggc gtcgggtgca gcgcggggcc ttggcgaatt gagggggcgg
     1321 accgccttgg aggaggcggt ccgcatgggc tgcgcttacc tgtccggatg ttttgtcccg
     1381 gtggagcggt tggggacggg ttagtgatcc aattgttttt ttattgcgat gatatcttcc
     1441 tctccgaagc gggcttcggc ttgctggaag gattgccagg ttacctcggc catcggggtg
     1501 tcccagccct cggcccgggc aaggcgcagg tcttttgcga tgagttttaa cgcgaaggcc
     1561 gccttatagt tatcgctgat gatggcgtcc ccttttattt tcccgaagac atttcccagc
     1621 gcactgttgt tcaggatggc catgaaatcc gcggttttga ctccgtggtg ctcggcgaag
     1681 atggccgctt cggcaagtcc ctgggcgtgg aagcccagca ggatgttgat ggccagtttg
     1741 gcggtattgc ccgagccggg gccgccgagg tgaagaacca gtttgccgag gtgttgaagg
     1801 atggggtttg ccttttcata ggccgtcccg gagccgcctg ccatgatcac cagttgtcct
     1861 tcctgtgcct gtttgacgct gccgcttacc ggggcgtcga ggtattcatt tccctgctgc
     1921 gcgcaagccg cggcgaattc cctgctgatc gcgggcgaga cggtgctcat gttgatgagg
     1981 agtttaccgg aggtctttgc gctcagcagg ccattgtcgc cggtgaacac gtcgcggata
     2041 gcctggtcgt cggagaccat gaggaagagg atatcggtgg cctttatcag atgggcgggc
     2101 gtgggggcaa tgccggcgcc ggcggcccgg aaggcggctt ctttttcctt gctgcgatta
     2161 tagacggtga cggagtaccc ggcgccgagg aggcgctggg ataagggggt gcccatgttg
     2221 ccgagaccga tccagccgat ggttgtgttg ttcattccgc aaagttgggg gaatcgggcc
     2281 ggttggccgt taccctttgg taaaaatgtc cgggttcggg ggggatggcg cggcggggta
     2341 gcgcggcgga atgttgtgtg atcgttaagt gcgtggcccg ggagtgacat ggtataggaa
     2401 ttgcaattgt cctccattaa aacaacccat gttccgcacc aatgtaaaaa aaccggtcac
     2461 cagggtgttg gtgatcgaag acgaaggaga tatgtgtttg ctgctcgaat tattactgga
     2521 tggcgaagga atggtcgtcg accatgtgcg ctcgctgtcg ggtgcgcggg agttcctcga
     2581 aaaagagcag ccttccctga tagtgttgga caaccggctg ccggatggat tggggatcga
     2641 ttttatcggt tacctgaaag cgcagtatcc ggcgataaag atcctgatga tctccggggt
     2701 ggatatcgag gcgaaagatg cggccctgga gatcggggcc gatacttttt tgtccaagcc
     2761 gtttacgcag tcgcagttga cgagcagtgt gagtagtttg ttgaattagc ggtgctgaag
     2821 gtccgggaaa taaaaaaagc ggtgatattt tttatttaag tagcaaattt gctacttttg
     2881 ttttattaaa ccggaccgat gaaaagttca gagctagtga ggaagtttat gcgtgcgggg
     2941 tggatagaga taagacagac cggaagtcac aaaatattca agaaccccaa aacaggcgat
     3001 acgatcccga taccttacca tgcgtcaaag gaagtgggca ttggcttagc cagcaagatc
     3061 cttaagaggg ccgggctcaa ataaatttca aaggccttat catttgttga aatataacag
     3121 gaaagatatg aagccaacca aaagcagcat ttcaaaaatc gaagtgatca ttgaaaagaa
     3181 tgacggcgtg ttgtggggaa gggttgaaaa tatgggggat catttgctga cgccttatgg
     3241 tgagagcacc aatgaggtga taaagaattt aaaagagttg gtgagggatt atcttgccca
     3301 tgaggggaag aaggataaat tttggaacag ggtagatatc aacaaggttg attttgagtt
     3361 cagctatgat ctgcaagcct tttttgccga gcacgattat ttgaagataa gttcggtggc
     3421 ggagtatgcc gggttgaacg cggggttgtt gaggcaatat gcttccgggg taaagttccc
     3481 gtcgcaggac caggccaaga agatagagga tgctatccat tatattggtg aggagttgcg
     3541 ggaaatatcg atctatgcat aggggccgac ggcgcgggag gctgttggcg gcgggtaaga
     3601 taaaaaaagc cgtaccggcg caaaccgata cggctgggga gaagtgtggg ccgggacatc
     3661 aatccagatc gatgtccagc cttatgcggc ggggccgttt gtcatgatcg tggtcgtcat
     3721 gatcgtgatg tccatggaat tcatggtgtt catgatgccc gtggtgttca tgttcttctt
     3781 cttcctcttc ctcctctatt tcttctattt cttcgaactc ttcctttatc tcgtgttccc
     3841 attccagggc cggctctttt agcgccgggt cgaggacata ggtgagtccg ccgacgacga
     3901 ggccttcgac gagaacgacg agccggatgg caacctcacc cgctgcggct acttcggcgg
     3961 cggtgaagtt ctggccaccg atcaggcgtg gccggatcat gcgggagtcg cgggggccaa
     4021 gggtgaatga tccgctgccc gggttggcga gttggatcgc ccagccacgg gtcgccagga
     4081 aggcgggaag gatggcccgg acttccacct taccggtctt tgccgtcggg ttgctggccc
     4141 agaaccggcg tttgcggaag gcggctacca gcgcgcgacg atggccaccg cccggaacgg
     4201 gtatgacggc gcgcagcccg atattattgt cgcagggcac caggcgccag agcggggatg
     4261 gaccggcggc agccggcagg agggtgaggg cgtcgtcatt ggcccggtcg ccaaaggcgc
     4321 ttacactcat gtacataccg tcgtgtccct tatattttgg tttccagtag aacggtcccg
     4381 ccagcacctc gccgccggga ggaatgacgc ccggaacggt aaaggaggtg gtcttcatcg
     4441 gctggaaatc atcgggccac acgaggccgg cgccgggccg gcagtgatag ccgtgtacga
     4501 ctacgccgct ggcggcctgg gtgccgcggt tgcggatgcg gacataggca tggtttatct
     4561 tcccgacaat gggggtttgg tgttcggcgt gaccgtcggc cgcatggcgg ttccatatct
     4621 cggtcgtctc ccaccatttc tgtaaatacg ggtattgatt ggccgggcca tattggtatt
     4681 cgccgtgtct gccgtcctcg atgaatacat caacgggcgg gggtgcgcct tccgtccaga
     4741 cgggggtcgg agcgcccagc ggctggaaga gtccctgttt ctcaaaggcc cagcggataa
     4801 ccttccagta gacgccgcca acctggtcgg cggtagtcca gttgccgagt tcggcactca
     4861 tcagatcggt ggcgtaggcc atggagttcg gcgggttggt gggctgcgtc agcatgccga
     4921 cggtacgcag gatcagatac ggtacatagc gaccggcgaa tttgcgcatc gccacctcgg
     4981 tggagtcgcc gccgatggcg cggtagatcc gaaaatgcgt tgtacacaag atctgttcgg
     5041 agttgtagcc gccggtgtcc attacgccgc cccagcccca gccggccgca acggcccggt
     5101 cgtggcgacg gccgatgttg acccagggga aggtgacata acgatcgggg gcgtgggagg
     5161 tggcatcgca gagaacggcg ccaaagctgt cgccggcgct gtgggcgaag ccgaagttgg
     5221 gcccgttgac a
//
LOCUS       DATGFG010000996         4385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_532482, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000996 DATGFG010000000
VERSION     DATGFG010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532482"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VKQ52_18890"
     CDS             <1..639
                     /locus_tag="VKQ52_18890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HLZ89332.1"
                     /translation="FVFFAYVLSLGKFFLIIAALDTGSSFEGMGASREALYSLLAEPA
                     FFILMGSFALLTGHTSFREIFGSLHFGTSISYAVGLLAAFVITIIALIENSRMPIDDP
                     RTHLELTMIHEVMILDNSGFDLGLLQYAVNLKFAMYGALIANFFLTPGSELYFSIPIF
                     IVTQLLFAVVIGIIESFMARFRMNHNPQYIFTLTSISLLIFLGVLLMLGKIV"
     gene            655..1284
                     /locus_tag="VKQ52_18895"
     CDS             655..1284
                     /locus_tag="VKQ52_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89333.1"
                     /translation="MTNILLIAFVISLVQMGLSKRLNTYTMVLALQGILLFGVAFIEL
                     IEINVVNLLFVLMETIGFKTIAIPYFLNYVIKKNKITRESDPFLPDFLSVVIITTIIL
                     GSFLLSNAIQDAHIRKIFFVTALSALFTGLYFIISRKKIITHVMGFLVIENGVFVLSL
                     AVGVEMPMLVNIGILLDIFASVLILGVFVNKIGDILKEQDVDQLRNLKD"
     gene            1306..2733
                     /locus_tag="VKQ52_18900"
     CDS             1306..2733
                     /locus_tag="VKQ52_18900"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HLZ89334.1"
                     /translation="MIGSYLIAVILISLALFLNRKKTLNYLFVALFVLLQWGLTVYEY
                     RHRDVQELGYFTPDALAVIFLLILSIISVPAFLHSFIYFNTEKDIPRQRSIYFAAMVV
                     LLAALSAAYLSDNIAITWIFVELTTLSASALIYHRRNVRTLEGTWKYVFVCSISITLV
                     FIGILFLTVAAQEVGVRNLNYKVLEAGAGTFNIFWLKLAFLFIFTGFTSKAGLVPMYT
                     VGIDAKDKAPSPAGALFGSVLMNVGFLGIFRFYNIISHTSIFPWAGKILLISGVLSIF
                     VATVYMLKVKNFKRMFAYSSVEHMGLIVLGLAAGGIGYYAAILHLILHSFAKSALFFQ
                     VGQVYRIYKSKSIYDAGDYFKYNIAGALVLLAGFFIVTAMPPSGMFISEFLIFRSLFE
                     SGHLWLLILVLILLTMIIWSFGKNIFKILFIKPVGFDETTVEKIRPVESLSQYIFLAL
                     VVYLGLNPPAEFVKLIQSAVAHLPK"
     gene            2735..4240
                     /locus_tag="VKQ52_18905"
     CDS             2735..4240
                     /locus_tag="VKQ52_18905"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89335.1"
                     /translation="MNTYSTIFNGQAVPLQTIPVLAYESFLELNTELVRSQECHCVNY
                     YGFAIGERIKLICCIADDAKHTIYVSSAEIARVGSGPIASLTARHLSFHIFERELQEN
                     FGIGYLDHPWLKPVRYAFNKYNPEDRIENFPFYRIHSEELHEVGVGPIHAGIIEPGHF
                     RFICNGEQILHLEIQLGYQHRGVEALFLEKKKLLQRITLAESISGDTVVGHTSAFVNI
                     WESLCGFRPEEELLFDRTLALELERLAIHTGDLSAICTDIGYQLGSSVFGRLRTPIIN
                     FMQEWCGNRLSKGLIRAGKTNFPFTPQIARRLSEVLDAFEPDFGEMYDEMKTLPTVLA
                     RLEKTGVVTAGQAGSIGAVGMTARASGIARDIRSSHPFALFGQLDHVPVVKRHGDVYS
                     RTQIRKDEILQSIGYIRKWLQNIPQGGERMSELSMPEPGLFVVSLIEGWRGEICHCAV
                     TGHQGELLHYKIKDPSLHNWLAVALAVRNNEISDFPICNKSFDLSYCGHDL"
BASE COUNT          990 a         1086 c         1073 g         1236 t
ORIGIN      
        1 tttgtttttt ttgcctatgt cctgtccctg gggaaattct tcctgatcat cgccgccctt
       61 gacacgggga gcagttttga aggcatgggc gccagccggg aggcattata ttccctgctg
      121 gccgaacccg ccttttttat cctcatgggg tcttttgcct tactcaccgg gcacacttct
      181 ttccgggaaa tattcgggtc tctgcatttt ggaacatcga tctcctatgc cgtcggcctg
      241 ttggctgcgt tcgttatcac cattatcgcc ctaattgaaa acagccggat gcccatcgac
      301 gaccccagga cacacctgga gctgacgatg atacatgagg tgatgatatt ggacaacagt
      361 ggtttcgatc tgggcctgtt gcaatatgcc gtcaacctga agttcgccat gtatggcgcc
      421 cttatcgcca atttcttcct taccccgggc tctgaattgt atttttccat acctattttt
      481 attgtgaccc agctcttatt tgcggtagtc atcggcatca tcgaatcgtt catggcaagg
      541 ttcaggatga accataatcc gcaatacatc tttaccctca cctcgatttc tttgctgatc
      601 ttcctgggcg tgctgctgat gcttgggaaa atcgtttaaa taaaaggaga aagaatgacc
      661 aatattttgc ttattgcttt tgtcatcagc ctggtccaga tggggttgtc aaaaagattg
      721 aatacctata cgatggtact cgccttgcag ggcattctgc tgtttggcgt agcttttatc
      781 gaactcatcg agatcaacgt ggtcaacctt ttattcgtgc tgatggaaac catcggtttt
      841 aaaactattg ccatccctta tttcctgaac tatgtgatca aaaagaacaa gatcacccgg
      901 gagtcggacc cttttctccc ggattttctt tcggtggtga tcattaccac gatcatcctt
      961 ggatctttcc tgctttccaa tgccatccag gatgcgcata tccggaagat atttttcgtg
     1021 accgctttgt ccgccttgtt taccggtctt tacttcatta tttccaggaa aaagatcatt
     1081 acccatgtaa tgggattcct ggtcatcgag aacggcgttt ttgtgctgtc cctggccgtg
     1141 ggcgtggaaa tgcccatgct ggtgaatatc ggcatcctgc tcgacatctt tgccagcgta
     1201 ctgatattgg gagtgtttgt caataagatc ggggacattc tcaaagagca ggatgtagac
     1261 caattaagaa atctgaaaga ttaaataaat aagcgcggaa taaccatgat cggaagctac
     1321 cttatcgccg taatattgat cagcctggcc ttgttcctta acaggaagaa gacactgaat
     1381 tatttattcg ttgccctttt cgttcttctc cagtggggtc tcaccgttta tgagtaccgg
     1441 catcgggacg tgcaggaatt gggctacttt actcccgatg cattagcggt aattttcctg
     1501 ctaattttga gtattatcag cgtgccggcc tttctacaca gttttattta cttcaatacc
     1561 gagaaagata ttccccggca gcgcagcatc tattttgcgg cgatggtcgt cttgctcgcc
     1621 gccctgagcg ccgcctacct gtccgataat attgccatta cctggatctt tgtcgagcta
     1681 acgacattga gcgcgtcggc gctgatctac caccggcgga acgttcgtac gctggaaggc
     1741 acctggaaat acgtatttgt ctgttccatc agcatcaccc tggtttttat cggcatcctc
     1801 tttcttaccg tggctgcgca ggaggtaggt gtcaggaatc ttaattacaa ggtattagag
     1861 gccggtgcag gtaccttcaa catcttctgg ctgaagctgg cattcctgtt catcttcacc
     1921 ggcttcacgt cgaaagcagg cttagtaccc atgtataccg taggcatcga tgcaaaggac
     1981 aaggctccct cgccggccgg agctttattc ggcagtgtgc tcatgaatgt gggcttcctc
     2041 gggatcttcc gtttctacaa catcatcagt cacacctcaa ttttcccctg ggccggcaaa
     2101 atattgctta tttcaggggt attgtccatt ttcgtcgcca cggtatatat gcttaaagtc
     2161 aagaatttca aacgcatgtt cgcgtactcc agcgtagagc atatgggttt gatcgtactg
     2221 gggctggcgg caggcggcat cgggtattat gccgccatcc tgcacctcat cctgcattcc
     2281 tttgccaagt ccgccctttt cttccaggta ggccaggtgt accgcattta taaaagcaaa
     2341 agcatctatg atgcgggaga ctatttcaaa tacaatatcg ccggtgcctt ggtattgtta
     2401 gccggatttt ttattgtgac tgccatgcct ccatcgggta tgttcataag cgagttcctg
     2461 atattccgtt cgctgttcga aagcggtcat ctctggttgc ttatccttgt ccttatcctg
     2521 ctgacgatga tcatctggtc gtttggaaaa aatatcttca agatcctttt tattaaacca
     2581 gtggggtttg atgagacgac ggtggaaaag atccggccgg ttgaatccct gagccagtat
     2641 attttcctgg cattggttgt ttacctgggg ttaaatcccc cggccgaatt tgtaaaattg
     2701 atccaatcag ccgttgcgca tttaccaaaa taacatgaat acctattcta ccatattcaa
     2761 tggacaagct gttcccctgc aaaccattcc cgtcctggcg tacgaatcgt ttctggagtt
     2821 gaatacggaa cttgtccgaa gccaggaatg ccattgtgtc aactattacg ggtttgcaat
     2881 aggggagcgt ataaagttga tctgctgtat tgccgatgac gcaaaacaca ctatttacgt
     2941 ttcctccgct gagatcgcca gggtgggctc agggccgata gcttccttga cagcccggca
     3001 tctttctttt catattttcg aacgcgagtt acaggagaat tttggcatcg ggtacctcga
     3061 ccatccctgg ttaaaaccgg tgcggtatgc ttttaacaaa tacaatcctg aagacaggat
     3121 cgaaaatttt cctttttacc gcatccacag tgaagaacta catgaagtag gggtgggacc
     3181 cattcatgca ggtatcatcg agcccgggca tttccggttc atctgcaatg gcgagcagat
     3241 ccttcacctg gagatccagc tgggctacca gcaccgcggc gtcgaggcgc ttttcctgga
     3301 gaagaagaaa ttgcttcagc gcattaccct ggcggagtcc atctccggcg acaccgtagt
     3361 cgggcatact tccgcctttg tgaacatatg ggaatccctc tgcggtttcc ggccggaaga
     3421 agagctgtta ttcgacagga cccttgcttt ggaactggaa cgtctggcta ttcacaccgg
     3481 tgatctcagc gcgatctgta ccgatatcgg ctatcaatta gggagttctg tcttcggcag
     3541 gctgcgcacc ccgatcatca attttatgca ggagtggtgc ggcaaccgcc tgtcaaaagg
     3601 cttgataagg gccgggaaga cgaattttcc tttcactccg cagattgccc ggaggttgag
     3661 tgaagtgttg gacgcctttg aaccggattt cggcgagatg tacgatgaga tgaaaaccct
     3721 gccgaccgtt ctggcaaggc tggaaaaaac aggagtggtc actgccgggc aggccggatc
     3781 tataggggcg gttggcatga cggcccgtgc atcgggcatt gcccgggaca tccggagctc
     3841 ccatcctttt gccttgttcg gacaattgga ccacgtcccg gttgtcaagc gtcatgggga
     3901 tgtctattcc cgcacgcaga tacggaaaga tgaaattctg caatccatcg gttatatccg
     3961 caaatggctt caaaatatcc cacagggagg cgaaaggatg tctgaacttt cgatgccgga
     4021 accgggttta ttcgtggttt ccctgataga aggctggcgg ggcgaaattt gtcattgcgc
     4081 agttaccggc catcagggcg aattgctgca ctataagata aaggaccctt cgttgcataa
     4141 ttggctggcg gtggcgctgg cggtgcggaa caatgagatc tcggatttcc ccatttgtaa
     4201 taaaagcttt gatctgagct attgcgggca tgatctgtaa caatataaac ccatcgacga
     4261 tgatcgacaa tcttaaaata ctccttcacc agggcaagca atatatcccc gatctcacaa
     4321 aggccggcgt accgggtatt ttcagggggc gcccggtgat cagcgctgcg gccgtcgaag
     4381 atcgg
//
LOCUS       DATGFG010000997         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_853663, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000997 DATGFG010000000
VERSION     DATGFG010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853663"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VKQ52_18910"
     CDS             complement(<1..747)
                     /locus_tag="VKQ52_18910"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ89336.1"
                     /translation="MKPIIRYSLIIAAIALIQSCKKDFLTRPPQDATTSGNFYTTDAE
                     VMAGTAPLYGIVWFDYNDKAFLSFGEAHGGNLNCDDADRKGYLSFSVPQTDDILPSGY
                     EAFWKIVAQSNLVITNITNASTTADAATKKMGIAECRFMRGLSYMFLVSNWGPVPILY
                     NNVTQLTDSTIKRNDLTSDWKLIIMDLVYAANHLPSTPYQQGRLTKWSAEGMLARAYL
                     YRSGLNQSLGSRSQSDLDSAKYYAADVISNS"
     gene            complement(772..>3370)
                     /locus_tag="VKQ52_18915"
     CDS             complement(772..>3370)
                     /locus_tag="VKQ52_18915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89337.1"
                     /translation="GGAAGSYNAPASYASPLAGINPDDIETINVLQGPSATAIFGAVG
                     GNGVVMITTKHGRAGESKITFNTMLTQQDLPKFAPVMNLPMYATFRNELAAAGGASTE
                     ADFADPSILGTGSNWQAALFRRTLQQKHDLAISGGSDKTTYYLSGEYFDQQGIAQGSG
                     FTRGSVRLNVESNARKWLKVGTNITGHITKERVVTSNNSLIETAIDISPAIPIKNADG
                     TWGGPPAGTPYANNFINPIALARLNTNYNKGFGGLGGVYVDISPIKGLVWHTEANGQY
                     DYLTNYQFNPAYTFGITVKSQITGSKQVSSNYWYGFHTRLQYDIKIHDHSISAMVGHE
                     AQVNGYETLSGYKDGFVNNTVQTLNAGASDLTQSNSSTMGSQSKESYFGRVVYIYKDR
                     YILQATERFDGSSAFGANNRWGHFPAVSAAWRISQEPFMKTITAIDDLKLRVEYGEVG
                     NAGSNGLAQYAVLSSYPTAFGTGFLPSNFPNPALQWEVDKTKNVGIDLHMFNSRLEVI
                     ADAYIKDLTKLLTYSTYPTYLGGGTSQGGLAWPETNVGSMQNKGFGVTVNTVNYQSHD
                     FLWKTGLNFSLDRNKVTQLTNTINAIYTTNTNSTQAQFLTKVGQPVGMITGYVAQGLF
                     QNYKDIVGHANQTSNTQVTVDPNTGTWVGDIKFKDVNGDGVINQKDRVVLGNPWPKYT
                     FGFNNFVSYKGFDLNIFLQGSVGNDIVNMQRYENILPGSKGAFENYYAAVINFARPTS
                     YNVADALTATLQNPNTTVPRVYTSTANGNERLTQWNVESGTYVRVKNISLSYNFPSKW
                     ITHMAMRSLRVGVNVQNAFTITKYKGYDPEVGPFNYWNSGNPIIINGLDNGRYPNVRM
                     YTANVIADF"
BASE COUNT          629 a          778 c         1136 g          827 t
ORIGIN      
        1 gctgttgctg atgacatcgg cagcatagta tttggcgctg tcgagatcgg attgcgaccg
       61 ggatcccagg gactggttga gcccgctacg atacaggtag gccctggcca gcatgccttc
      121 ggccgaccat ttggtgagac ggccctgctg gtatggcgtg ctgggaagat gattggcggc
      181 atagacgagg tccatgatga tcagcttcca gtcgctggtc agatcgttgc gcttgatggt
      241 cgagtccgtg agctgggtaa cgttgttgta gaggatgggt acgggtcccc agttgctgac
      301 gaggaacata taggacaggc cgcgcatgaa gcggcattcg gcgatgccca ttttcttcgt
      361 ggctgcgtca gcggtggtgc tggcgttggt gatattggtg atgaccaggt tgctctgggc
      421 cactatcttc cagaaggcct cataccccga aggcaggata tcatcggtct gggggacgga
      481 gaaggagaga taacccttac ggtcggcgtc gtcacagttg aggttgccgc cgtgggcctc
      541 gccgaagctg aggaaggcct tatcgttgta gtcgaaccag acgatgccgt agaggggggc
      601 ggtgcccgcc attacctcgg cgtccgtagt atagaagtta cccgaggtgg tggcatcctg
      661 agggggacgg gtgaggaaat cctttttaca gctttggatc agggctatcg ctgctatgat
      721 gagggaatat cttatgatcg gtttcattat caacaagctt ttagatttga attaaaaatc
      781 ggcaatgaca ttggcggtgt acatccggac gttgggatag cggccgttgt cgaggccatt
      841 gatgatgatg gggttgccgc tgttccagta gttgaacggt ccgacttcgg gatcatatcc
      901 tttatatttg gtaatggtga acgcgttctg tacgtttacg cctacccgca gactccgcat
      961 ggccatatgt gttatccatt ttgacggaaa attgtaggaa agggagatgt tctttacccg
     1021 tacgtatgtc ccgctttcca cattccactg ggtgaggcgt tcgttaccgt tggcggtgct
     1081 ggtatagacc cggggcacgg tggtgttcgg gttttgcagg gttgccgtca gggcatcggc
     1141 cacattatag ctggtgggcc gggcgaaatt gataacagcg gcatagtagt tctcgaaggc
     1201 gcctttgctg ccgggcagga tattctcgta tcgctgcata ttgacgatgt cattgcccac
     1261 ggagccctgg aggaagatgt tgaggtcgaa acccttatag ctgacgaagt tgttgaaccc
     1321 gaaagtatat ttcggccagg gattgccgag gacgacgcgg tctttctggt tgatgacgcc
     1381 atcgccgttg acgtccttga atttgatgtc acccacccat gttccggtgt tggggtcgac
     1441 ggtgacctgg gtattcgagg tctggttggc gtggccgacg atgtccttgt agttctggaa
     1501 gagaccctgg gcgacgtagc cggtgatcat gcctacgggc tggcccactt tggtaaggaa
     1561 ctgggcctgt gtactattgg tgttggtggt atagatggcg ttgatggtat tggtcaattg
     1621 ggtgacctta ttgcgatcaa gggagaaatt gaggcctgtt ttccagagga agtcgtggct
     1681 ttggtagttg acggtgttga ccgttacacc gaagcctttg ttctgcatgc tacccacgtt
     1741 ggtctcgggc caggccagtc cgccctggct tgtaccgccg cccaggtagg tggggtaggt
     1801 ggaatacgtg agcagcttgg tgaggtcttt gatataagcg tcggcgatga cttcgaggcg
     1861 gctgttgaac atatggagat cgatgccgac attcttggtt ttatccactt cccattggag
     1921 ggcggggttg ggaaagttgg agggaaggaa gcccgtgccg aaggcggtag gatagctgct
     1981 caggacggcg tattgggcca ggccgttgga accggcattg ccgacctcgc catattccac
     2041 gcgcagcttg aggtcatcga tggccgttat ggtcttcatg aaaggttcct gcgagatgcg
     2101 ccaggcggcg gataccgcgg ggaaatggcc ccagcggtta ttggctccga aagcggaaga
     2161 accgtcgaag cgttcagtag cctggaggat atagcggtct ttatagatat agaccacgcg
     2221 accgaaataa gactctttgg attgggagcc catggtgctg ctgttgctct gggtgagatc
     2281 ggaagcgccg gcattgagcg tctgaacggt gttgttgacg aaaccgtcct tatagccgga
     2341 caatgtctca tagccattca cctgcgcctc gtgacctacc atcgccgaga tactgtggtc
     2401 gtgaatcttg atatcgtatt gcagacgggt gtggaaaccg taccagtagt tggagctcac
     2461 ctgtttggag ccggtgatct ggctcttcac cgtgatgccg aaggtatagg cggggttgaa
     2521 ttggtagttg gtgaggtaat cgtattggcc gttggcctcc gtatgccaga ccaggccttt
     2581 tatggggctg atgtctacgt atacgccacc gagaccgccg aagcccttgt tataattggt
     2641 gttcagccgg gcgagggcga tggggttgat gaagttgttg gcatagggag tacccgcggg
     2701 agggccgccc catgtgccgt cggcgttctt aatgggaatg gccgggctga tgtcgatggc
     2761 ggtctcgatg agggagttat tggaagttac gaccctttcc ttggtgatat ggccggtgat
     2821 gttggtgccc accttcagcc atttgcgggc attgctctcg acgttgagcc ggacggagcc
     2881 gcgggtgaag ccggagccct gggcgatgcc ctgctggtcg aaatattcgc cggagagata
     2941 gtaggtggtc ttatcggagc caccgctgat agccaggtcg tgtttctgtt ggagggtacg
     3001 acggaagagg gctgcctgcc agttggagcc ggtccccagg atggatgggt cggcgaagtc
     3061 ggcttcggtg ctggcgccgc cggcggccgc cagctcgttg cggaaggtgg catacatggg
     3121 caggttcatc acgggggcga acttcggcag atcctgctgg gtgagcatgg tgttgaaggt
     3181 gatcttcgat tcaccggcgc gcccatgttt ggtggtgatc atgacgaccc cgttgccgcc
     3241 gacggcgccg aagatggcgg tggccgatgg cccctggagg acgttgatcg tttcgatatc
     3301 gtcggggttg atgccggcca gtgggctggc ataggatgcc ggggcgttgt aggagccggc
     3361 cgcgccgccc
//
LOCUS       DATGFG010000998         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1540490, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000998 DATGFG010000000
VERSION     DATGFG010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540490"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VKQ52_18920"
     CDS             complement(<1..995)
                     /locus_tag="VKQ52_18920"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HLZ89338.1"
                     /translation="MHILIAPNAFKNSLNATDAATAIERGLRQSRLNFTAECFPVGDG
                     GDGTGDLLIKKLGATLIGAPAHDPLGRLRQATFGFITKTNTAIIEMASASGIRLLKKE
                     ELDPLRTRSTGTGDLIRAALDHDARHIIIGMGGSATVDGGVGILTALGARFPAGPTNT
                     AGLTDTTGPTNTTDSTNTPNAIDTSGLDPRLATVKITVLCDVNNPLLGPRGAAAVFGP
                     QKGATPQAVQQLESTLQNLAGLIHKTTGKDIATLPSGGTAGGAAAGLYGILDAELVNG
                     IDYFLHLTGFDQALENTSLVITGEGSIDEQTLQGKAPFGVAVHAKKKGLFVIGMAG"
     gene            complement(1037..2002)
                     /locus_tag="VKQ52_18925"
     CDS             complement(1037..2002)
                     /locus_tag="VKQ52_18925"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HLZ89339.1"
                     /translation="MKPKIIAIDGYTLNPGDLSWQQWQPYGNLTVYDRTPYETTAIAG
                     RCAEAVAVLTNKTPFNAATLAQLPHLKYIGVTATGYNMIDIAAAKKQDIIVTNVPGYG
                     TVSVVQMTFALLLELTQRVQRHSDAVMEGKWSRSPDFSFWDYPLVELTGKTIGIIGMG
                     HIGSKVADIAAAFGMKIIGNSRTRTDQSHRPGFTWASIPELLAQSDVVSIHCPLFPET
                     LGLINSQTLSLMKPTAFLLNTSRGPIIVEQDLADALNTGRIAGAGLDVLSTEPPPAGN
                     PLFKAKNCIITPHISWATKEARTRLMAATLANFTAWQSGHPINVL"
     gene            complement(1999..>2527)
                     /locus_tag="VKQ52_18930"
     CDS             complement(1999..>2527)
                     /locus_tag="VKQ52_18930"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HLZ89340.1"
                     /translation="PLSGLTPKQGARVFIDTLRQLRFPILTIATVLAFAYLMNDSGIT
                     QTIAGALANTGPLFPFFAPILGWLGVFITGSDTSSNALFGKLQAATATSIGVDPVVTV
                     GANVSGGVIGKMISPQSIAVAAAAGHLVGKESDLFRFTVRHSFILLCIICCIVLAQAY
                     LFKWMIPAYTHLHHL"
BASE COUNT          427 a          597 c          963 g          540 t
ORIGIN      
        1 ccggccatgc cgataacgaa caggcctttc ttttttgcgt ggacggctac gccgaagggg
       61 gctttgccct gtagggtctg ttcgtcgatg ctgccttcgc cggtgatgac gagactggtg
      121 ttttccaggg cctggtcgaa gccggtgagg tggaggaaat agtcgatccc gtttacgagt
      181 tcggcgtcga ggatgccgta taagccggcg gctgcgccgc cggcggtgcc gcccgagggg
      241 agggtggcga tatcttttcc tgttgtttta tggatgaggc cggccaggtt ttgcagggtc
      301 gactcgagct gctgaacggc ctgtggggtg gcgccttttt gcgggccgaa gacggcggct
      361 gcgccgcggg gtccgaggag ggggttgttc acgtcgcaga ggacggttat tttgacggtg
      421 gcgagccggg ggtcgaggcc gctggtgtcg atggcgttgg gggtgttggt ggagtcggtc
      481 gtgttggtgg ggccggttgt gtcggtgagg ccggctgtat tggtggggcc ggctgggaag
      541 cgggcgccca atgccgttag gatgcctacg cctccgtcga cggttgccga gccgcccatg
      601 ccgatgatga tgtggcgggc gtcgtggtcg agggccgcgc ggatgagatc gccggtaccg
      661 gtggagcggg tgcgaagggg gtccagctct tcttttttca gcaggcggat accggaggcg
      721 ctggccattt cgatgatggc ggtgttggtt ttggttatga agccgaaggt ggcctgccgc
      781 agccggccga ggggatcgtg ggccggcgcc ccgatcaggg tggcgccgag ttttttgatg
      841 agcaggtcgc ccgtaccgtc gccgccatcg ccgacgggga agcattcggc ggtgaaattc
      901 agccggcttt gccgcagccc tctttcgata gccgttgcgg cgtcggtggc gttgaggcta
      961 tttttgaagg catttggggc gatgaggata tgcatggcgg agagatgagg atttagctgt
     1021 cagacattga tgaagtttat aggacgttga tggggtgacc ggattgccag gcggtgaaat
     1081 tggccagggt ggccgccatc aggcgggtcc gggcttcctt tgtggcccag gagatatgcg
     1141 gggtgatgat acagttcttt gccttgaaaa gcgggttacc ggctggtggc ggttcggtgg
     1201 aaagcacgtc caggcccgcg cctgcgatgc gtccggtgtt cagtgcgtcg gcgaggtcct
     1261 gttccacgat gatgggtccg cgggaggtgt tcaggagaaa ggcggtgggt ttcatcaggg
     1321 atagggtctg actgttgatc agcccgaggg tttcggggaa gagcgggcaa tggatgctta
     1381 ccacgtcgga ctgggcgagc agttccggga tggaggccca ggtgaaaccg gggcgatggg
     1441 actggtctgt ccgggtacgg ctgttgccga tgatcttcat gccgaaggcg gcagcgatat
     1501 ccgccacctt gctgccgatg tggcccatgc cgatgatgcc gatggtcttg ccggttagtt
     1561 cgacgagggg atagtcccag aagctgaagt ccggggagcg cgaccatttt ccttccatca
     1621 cggcgtcact gtggcgctgg acgcgctggg tcagctcgag gaggagggca aaggtcatct
     1681 ggactaccga gacggtgcca tagccgggga cattggtgac gatgatgtcc tgcttttttg
     1741 cggctgcgat atcgatcatg ttgtagccgg tggcggtgac cccgatatat ttcaggtggg
     1801 gaagttgcgc cagggtagcc gcgttgaacg gggtcttgtt cgtgagtacc gcgacagcct
     1861 ccgcgcagcg tccggcgatg gccgttgttt cgtaaggggt ccggtcatag accgtgaggt
     1921 tgccgtatgg ctgccattgt tgccaggaga ggtcaccggg gttcagggta tagccgtcga
     1981 tggcgatgat ctttggtttc ataagtgatg taaatgtgta taggccggta tcatccactt
     2041 gaacaggtag gcctgggcca ggacgataca gcagatgatg cacaacagga taaagctatg
     2101 gcggaccgtg aaccggaaga gatcggattc ttttccgacc aggtgaccgg ccgccgccgc
     2161 cacggcgatg gactgcgggg agatcatctt tccgatgacc ccgccggaga cattggcgcc
     2221 cacggtgacg accgggtcga cgccgatgga ggttgccgta gcggcttgca atttaccgaa
     2281 aagcgcattc gatgaggtat ctgaaccggt gataaagacg ccgagccagc caaggatggg
     2341 agcaaagaag gggaagaggg ggccggtgtt ggccagggcg ccggcgatgg tctgggtaat
     2401 gccggagtcg ttcatcaggt aggcgaaggc cagcacggtg gcgatggtga ggatgggaaa
     2461 acgcagttgg cggagggtgt cgatgaagac ccgggcgccc tgctttgggg tgaggccgga
     2521 caagggc
//
LOCUS       DATGFG010000999         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1195733, whole genome
            shotgun sequence.
ACCESSION   DATGFG010000999 DATGFG010000000
VERSION     DATGFG010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195733"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VKQ52_18935"
     CDS             complement(<1..1171)
                     /locus_tag="VKQ52_18935"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HLZ89341.1"
                     /translation="MWESIARLVLKFRLFWLVALLSVTGYMAFRASRVKLSYDFNSAI
                     PTDNPKYKAYQEFRKTFGEDGNLLVIGVQSGKLFQLPVFTDYIAMTEALKRVPGVDDV
                     LGVSSAVSLVRDSVEEKMKAVPIFPAHPASQAEIDSGKAMLLGLPFYEGLLYNSQTGT
                     WLMAVHVNKAVMNSAQRIVTVNGITRLVDSFGVRNGLDMHYSGLPLIRTRMAIKVASE
                     TTWFLLGSVVMLSMILLLFFRSISTMLLSLAVVIIGVVFSFGTLDLLGYKITLLNALT
                     PTLVVVIGIPNCIYFMNKYHTAYRDTGDKQEALVVMIGRMGVVTLFCNLTAAIGFGVF
                     ALTRSAILNEFGVVAGINIMLLFFISFILIPVVLSYLPAPRSKHMLYLQNRWLAAV"
     gene            1213..1953
                     /gene="recO"
                     /locus_tag="VKQ52_18940"
     CDS             1213..1953
                     /gene="recO"
                     /locus_tag="VKQ52_18940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HLZ89342.1"
                     /translation="MQLHKTKGIVLRGVKYGETSLVVTLYTELFGIQSYLVNGVRTSS
                     RKGPGKANLFQPAAILDLIVYHNELKNLQRIREFKWAVLYQHIFFDVIKNAVAVFMVE
                     LLQKCIKQPEPNPDLFYFIEDAFLHLDAAESRVLANFSLFFMLHLAGFFGFKIEDTYS
                     ERNPFLDLVEGQFVAHHPQHPQALEGPLSYITAQLLRVQQPAELRELALNQETRRNLL
                     QAYKAFYALHVPDFGEMRTLNVLQTVLS"
     gene            complement(1950..3122)
                     /locus_tag="VKQ52_18945"
     CDS             complement(1950..3122)
                     /locus_tag="VKQ52_18945"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ89343.1"
                     /translation="MTYLKFRADDLFTGEGFAGPDSVLVTSGEGVVQAVVPAAAAGED
                     VVYYPGVLSPGFVNCHCHLELSGMRGVIPEGTGLVDFLSAVVRGRGSARPGDTGDQGD
                     AIAAGEQEMLDNGIVAVGDICNGADTLIQKRKGRLRYSNFIETMGFIEGGAKMRFDHS
                     LRVLEELRELGAGVIVPHAPYSVSPALFRMIAGHAGSGRVLSIHNQETEAENVFLSSG
                     EGDFLRLYAALGLDVSFFRGTGKRSLASWLGYIDKAQPMIVVHNVATREEDLGAAEGY
                     DLHFCLCPNANLYIGGQLPDVGLLVRKGCSLVVGTDSLASNHALSILGELKTLQRAFP
                     ELGTPQLLRWATLNGARALGMEGELGSFGAGKRPGVVLLEGMREGRLEGARVKRLV"
BASE COUNT          793 a         1136 c          710 g          588 t
ORIGIN      
        1 acacggccgc cagccagcgg ttctgcaggt acaacatatg cttcgacctg ggcgccggca
       61 gatagctgag caccacgggg atcaggataa aagaaataaa gaacaacaac atgatattga
      121 tcccggccac aaccccaaac tcattcagga tagcgctccg cgtcagcgca aacaccccaa
      181 aaccgatggc cgccgtcagg ttacagaaca gcgtaacgac acccattctg ccgatcatca
      241 ccacaagggc ctcctgctta tcacccgtat cccggtaagc cgtatggtat ttattcatga
      301 aatagataca attgggtatc ccgatgacga cgacaagggt gggggtgagg gcattcagta
      361 acgtgatctt atatcccagc agatccaagg tcccaaaact gaaaacgacc ccgatgatca
      421 ccaccgccag cgacaacagc atcgtactga tgctccggaa gaaaagcaaa aggatcatcg
      481 acaacatcac caccgacccc agcaggaacc acgtggtctc ggaagcaacc ttgatggcca
      541 tcctggtccg gataagcggc aaaccactgt aatgcatatc cagcccgtta cgcaccccga
      601 aactgtccac cagccgggtg atgccgttca ccgtcacgat ccgctgcgcc gagttcatca
      661 cggctttgtt gacatgcacc gccatcagcc aggtccccgt ctggctgtta tacaaaagac
      721 cctcgtaaaa aggcagaccc agcaacatcg ccttgccact gtcgatctcc gcctgcgaag
      781 cggggtgcgc gggaaagatc ggcacggcct tcatcttctc ctccaccgaa tccctcacca
      841 ggctcacagc cgaagacaca cccagtacat cgtcgacccc cgggacccgc ttcaacgcct
      901 ccgtcatcgc gatatagtcc gtaaagacag gcaactgaaa aagcttcccg gactgtacac
      961 cgatgaccag caggttgccg tcttccccaa acgtcttccg gaattcctgg taggccttgt
     1021 atttgggatt atccgtgggg atcgcgctgt tgaaatcata actcagtttc acccgggagg
     1081 cccggaaagc catataacca gtgacggata acaatgctac aagccagaac aaccggaatt
     1141 tgaggaccag acgggcaatg gattcccaca tgtatgaaaa tttttactct gcaaataaac
     1201 gtatttttac aagtgcaatt acataagacc aaaggaatcg tcctcagggg ggtgaaatac
     1261 ggcgaaacaa gcctcgtcgt caccctctac accgaactct tcggcatcca gtcctacctc
     1321 gtcaacggcg tccgcacaag ctcccgcaag ggcccgggta aggccaacct cttccagccc
     1381 gcggccatcc tcgatctcat cgtctatcac aacgagctga aaaacctgca acgcatccgc
     1441 gaattcaaat gggccgtcct ctaccagcac atcttcttcg atgtgataaa gaatgccgtc
     1501 gccgtcttca tggtggaact cctccagaaa tgcatcaaac aaccggaacc caacccggac
     1561 ctgttctact tcatcgaaga cgccttcctc cacctcgacg ccgccgaaag ccgcgtcctg
     1621 gcgaatttct cgctgttctt catgctccac ctcgccggct tcttcggttt taagatcgaa
     1681 gacacctaca gcgaacgcaa ccccttcctc gacctggtgg aaggccagtt cgtcgcccat
     1741 cacccccagc atccacaggc gctcgaaggc ccgcttagct atatcacggc ccagctgctt
     1801 cgcgtacaac aacccgccga actccgggaa ctcgctctca accaggagac ccgccgcaac
     1861 ctcctccagg cctacaaggc cttctatgcc ctccacgtcc ccgacttcgg cgagatgcgc
     1921 accctcaacg tgctgcaaac cgtcctttcc tagaccaatc tcttcaccct ggcgccttcc
     1981 agccttccct ccctcatccc ctccagcaac accacacccg ggcgcttgcc tgccccaaaa
     2041 cttcccaact ccccctccat ccccagcgca cgcgccccat tcagcgtcgc ccaccgcagg
     2101 agctgcgggg tccccagttc cgggaaagcc cgttgcaagg tcttcaactc cccgaggata
     2161 ctcaaggcat ggttcgacgc caggctgtcc gtaccaacga caagggagca gcccttgcgt
     2221 accagcaacc ccacatccgg cagctgcccg ccgatataca ggttggcatt cgggcacaaa
     2281 caaaaatgca ggtcatatcc ttccgcagcc cccagatcct cttcccgcgt agccacattg
     2341 tgtacgacga tcatcggctg cgccttgtca atatagccca gccagctggc caggcttcgt
     2401 ttacccgtgc cccggaaaaa ggaaacatcc agccccagcg cggcatacag ccgcaggaaa
     2461 tccccttccc ccgaagacag gaatacattc tcggcttcgg tctcctggtt gtggatcgac
     2521 aagacccgcc cgctccccgc atgcccggcg atcatccgga aaagcgccgg cgacaccgaa
     2581 tagggcgcat gcggaacgat gacaccggcc cccagctccc gcagctcctc cagcacccgc
     2641 aggctatggt caaaccgcat cttcgcccca ccctcgataa accccatcgt ctcgatgaaa
     2701 ttactatagc gtaaccgccc cttccgcttc tgaataagcg tatcggcccc attgcagata
     2761 tcccccaccg ccacgatgcc attgtccagc atctcctgct cacccgccgc gatcgcatcc
     2821 ccctgatcgc ctgtatcacc gggacgggcc gaccctcgcc ccctgaccac cgcggaaaga
     2881 aaatccacca gccccgttcc ctccgggatc acgccccgca tccccgacag ctcgagatga
     2941 caatgacaat tgacaaaacc cgggctcaaa acccccgggt aataaaccac atcttcaccc
     3001 gccgccgcag ccgggaccac agcctgcacc accccctcac ccgaggttac cagcacgctg
     3061 tccggccctg cgaaaccctc ccccgtaaat agatcatcgg ccctgaattt taggtaagtc
     3121 attcgtaaac aatttatctt tgcgtttcgc aagcagcgac ccaaatatat gctagataaa
     3181 ctggaagcca taaaagcccg attcgaagac ctgggagtag ccctgag
//
LOCUS       DATGFG010001000         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_404421, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001000 DATGFG010000000
VERSION     DATGFG010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404421"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VKQ52_18950"
     CDS             complement(<1..761)
                     /locus_tag="VKQ52_18950"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HLZ89344.1"
                     /translation="MDAIAAKALLQRYLEGNASQEEMIIVEQWYQQLVDVGEWTWAEG
                     EREQLQAAMEARLLQRIASPRIGAKWLILRRLSAAAILLLAVASVWVLITRRTQQPLA
                     SMSQRYRNDVRPGRNAAVLTLAGGKTIVLDDSAPKTIGAQGNTVILNDSGQLVYEASS
                     RPVPSGKASEIFYNTLTTQKGNQYHLTLPDGTNVWLNAVSSITYPTAFTGNQRRVTIS
                     GEAYFEVMKNQQQPFIVQQGDITVQVLGTSFDVNG"
     gene            complement(864..1457)
                     /locus_tag="VKQ52_18955"
     CDS             complement(864..1457)
                     /locus_tag="VKQ52_18955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HLZ89345.1"
                     /translation="MKDTVLDSDEALLDRMADDDQAAFTALYRRHWETLFITTVRVIG
                     NREDAEDIIQEVFASLWRRRKALNLTGPLAGYLQVSVKYKAISYIDRNITRRHYLVAL
                     GKAAESAVPASPEVLLRVKEVQQLIQTVIENMPPKMREVFQLSRREHLTHREIAARLG
                     ISEETVKKHIQNALQLLRSAMGKAPASVPALLFYLLS"
     gene            complement(1514..2272)
                     /locus_tag="VKQ52_18960"
     CDS             complement(1514..2272)
                     /locus_tag="VKQ52_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ89346.1"
                     /translation="MNIVIIEDEMVSSEDLAEMIRQIQEDVTLRAILHSVKEAVAFFK
                     KEPDVDLIFSDIQLGDGLSFEIFRQMTIDAPIIFCTAYNDYAIEAFKTNSIDFILKPF
                     NSDSVAAAIAKYHQLKKHFSPRQFDYDLLIRQLTGNKKGVSSILIHYKEKVVPIGLED
                     IALFYIERDVTRMLCFDGRTFLADQTLDQLERICDDQFFRVNRQVLVNRKAVKDASHF
                     FPRKYIANISVPFKDPVTVSKNRTTAFLEWLQKK"
     gene            complement(2269..>3259)
                     /locus_tag="VKQ52_18965"
     CDS             complement(2269..>3259)
                     /locus_tag="VKQ52_18965"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ89347.1"
                     /translation="AVGVINALLLALCVVLILSLGLGRKVNDVVLRKLVAVFIGNLLC
                     WAINIVNYIFLSPVLLKRGFAGKALYYSASYVPSVLAIALLAGPVFTMLGLSRLRDPY
                     APYAWGAGINSVALLAIELIVSRYERISMRLENAELRMMTLQAQHEKLKNQLHPHFLF
                     NSLNVAKALIRRNPVLAENYIIKLSDFLRFSISHNEHNIVPLKEELKFSLYYLEMQKV
                     RFRDSLIYEVDESLDTMDGAMLPVFSLQLLLENAIKHNTLTTESPLVIRIGHAAPDWL
                     VVTNNRSRRLNLEEGTGVGLKNLADRYKLLSGEEMKIDIDNGSFRVYIKLLSK"
BASE COUNT          701 a          876 c          908 g          774 t
ORIGIN      
        1 tacccgttga catcgaagct cgtgcctaat acctgaacgg tgatatcgcc ctgctgcacg
       61 ataaagggct gctgctggtt tttcattacc tcgaaatagg cttcgccgct gatggtcacc
      121 cgacgttggt tgccggtgaa tgccgtagga taggtgatgg aggagacggc attcagccag
      181 acgttggtgc cgtccggtaa ggtcagatgg tactgatttc ctttttgcgt ggtcaacgta
      241 ttataaaaga tctccgacgc tttccctgac ggcaccggcc gggatgatgc ctcgtacacc
      301 agctgcccgc tgtcgttgag gatgacggtg ttgccctggg cgccgatggt ttttggggcg
      361 ctgtcatcga gcacgatcgt cttgccgcct gccagtgtca gcacggcagc attccgaccc
      421 ggccttacgt cattccggta ccgttggctc atcgaggcca gcggttgttg cgttctccgg
      481 gtaatgagta cccagacgct tgccactgcc agcagcaaga tggctgctgc gcttagtctt
      541 cgcaggatga gccatttagc gccgatgcgg ggagaagcga tgcgctggag caggcgtgct
      601 tccatggctg cttgcagttg ttcccgttct ccctcggccc aagtccattc cccgacatcg
      661 accaactgtt ggtaccattg ttctactatg atcatctctt cctgactcgc attgccctca
      721 aggtatcgct gcagcagggc tttggcggct attgcatcca tagataagtt gttacgtata
      781 tatagtccgg tgaagaaagc cgaaggggta ataaaaagat aataattttt ttacccaggg
      841 tttatgatcc taaggagagg tctttacgat agcaggtaga aaagaagcgc gggaacggag
      901 gcgggggcct tgcccatggc cgaccggagc aattgcaggg cgttctggat atgctttttg
      961 accgtttcct cggagatgcc gagccgggca gcgatctccc tgtgggtcag atgctcccgg
     1021 cggcttagct ggaacacttc ccgcattttg ggcggcatgt tctcaatgac ggtttggatc
     1081 agttgctgga cctccttcac ccgcagcagc acctcgggcg atgccggaac cgcgctttcc
     1141 gcggctttgc cgagtgctac caggtagtgg cgccgggtga tattcctgtc gatatagctg
     1201 atcgccttat acttgacgct gacctgcagg tagccggcca gcgggccggt gaggttcagc
     1261 gccttacgcc ggcgccaaag cgatgcaaag acttcctgga tgatgtcttc cgcatcttcc
     1321 ctgttcccaa ttacccggac ggtggtgata aagagggttt cccagtgccg ccggtagagc
     1381 gccgtgaagg ctgcctgatc atcgtccgcc atcctgtcca ggagggcttc atcgctgtcc
     1441 aaaaccgtat ccttcaataa attctacatt ttggataaaa gtaataaaaa tcggagggtt
     1501 ccccgcatac gccctatttt ttctgcagcc attccaggaa ggccgtcgtc cgattcttgc
     1561 tgaccgtgac cgggtctttg aagggcacgg aaatgttcgc gatgtatttc ctcggaaaga
     1621 aatgggaagc atcttttacc gccttccggt tgaccaggac ctgccggttg acccgaaaga
     1681 actggtcatc acagatcctt tccagctgat cgagggtttg gtcggccaga aaagtccgcc
     1741 catcgaagca aagcatccgc gtgacatcgc gttcgatata aaaaagggcg atatcctcca
     1801 acccgatggg caccaccttt tccttgtaat ggatcaggat ggagcttacc ccctttttat
     1861 tgcccgtaag ttggcggatc agcaggtcgt agtcgaattg ccgcggggag aaatgctttt
     1921 tcagctgatg gtacttggca atggccgcgg ccaccgagtc gctgttgaac ggtttgagaa
     1981 taaaatcgat gctattggtc ttgaacgcct cgatcgcata gtcgttatag gcggtgcaga
     2041 aaataatcgg cgcgtcgatg gtcatctgcc tgaagatctc gaagctgagc ccatccccca
     2101 attggatgtc cgaaaagata aggtcgacat ccggttcctt tttgaaaaag gcgaccgcct
     2161 cctttacgga atggagaatg gcccggagag tcacgtcttc ctggatctgc cggatcattt
     2221 ccgccagatc ttcactgctg accatctcat cctcgatgat gacgatattc attttgaaag
     2281 gagtttaatg tacacccgga aagacccgtt atcgatatct atcttcattt cttcgccgga
     2341 caagagctta tagcggtcgg ccaggttctt cagccccacc ccggtgcctt cctccaggtt
     2401 gagccgccgg gatctgttgt tcgttacgac caaccagtcg ggcgccgcat gaccgatgcg
     2461 gatgacaagc ggggattcgg tggtgagggt attgtgtttg atcgcgttct ccagcagcag
     2521 ctgcagggag aacacgggaa gcatggcccc atccatcgtg tcgagggatt cgtcgacctc
     2581 gtagatcagg ctgtcccgga accggacctt ttgcatctcg aggtagtaca gggagaattt
     2641 cagctcttcc ttcaacggga cgatattgtg ttcattatgc gaaatggaga agcgcaggaa
     2701 atcggagagc ttgatgatat aattctccgc cagcaccggg ttcctgcgga tcagcgcctt
     2761 ggctacattg agagaattga agaggaagtg cgggtgcagc tggttcttca gcttctcatg
     2821 ctgcgcctgg agggtcatca tgcgtagctc tgcgttctcc agccgcatgc ttatccgttc
     2881 gtacctcgag acgatgagtt caatggccag gagagccacc gaatttatcc cggctcccca
     2941 tgcatacggc gcgtaggggt cgcgaagcct gctgaggccc agcatcgtaa agacgggacc
     3001 ggccagcagg gcgatggcca gcaccgacgg aacataactg gccgaatagt acagggcctt
     3061 acctgcgaaa ccccttttca acagtacagg cgacaaaaag atataattga cgatattgat
     3121 cgcccagcac agcaggttgc caatgaacac ggcgaccagt ttccgcaata ctacgtcatt
     3181 caccttccgg cccaatccca gcgataggat cagcacgaca caaagggcga ggagcaaggc
     3241 gttgatcaca ccgaccgcc
//
LOCUS       DATGFG010001001        10349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1540698, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001001 DATGFG010000000
VERSION     DATGFG010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540698"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VKQ52_18970"
     CDS             complement(<1..211)
                     /locus_tag="VKQ52_18970"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLZ89348.1"
                     /translation="MNIPSCTYRIQLNDRFTLRDLQALLPYLHELGISTIYASPLTTA
                     LKGSQHGYDVTDSLVLSPDIGTEEQW"
     gene            complement(264..1235)
                     /locus_tag="VKQ52_18975"
     CDS             complement(264..1235)
                     /locus_tag="VKQ52_18975"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLZ89349.1"
                     /translation="MNEVAISKRLAVGVFITLAVLILIAAIFTIGGQHKAFVRSVRVH
                     ILFDDVQGLQPGNNVWLSGMKIGTVRSIDFYHKSQVAVTLNIEKKAQPHIADDAKARI
                     STDGLVGNKIVVISGGSDNARPIVDNDSLLSEHLAGTQEMFATLQASNANLLEMTGNL
                     KTISKKLNEGQGTLGKLISDPSIADHLRNSIDHLRTAAANSENLIANLEDFSARLKQP
                     DGLASQLATDTTVFNRIKGAVARLNEAATQATDFTISLRDAGAGLNDKNSPAGTLLHD
                     EDAGADLQRTLKNLRVSSKELSDDLEALQHNFLLRGFFRKREKEQKE"
     gene            complement(1232..1993)
                     /locus_tag="VKQ52_18980"
     CDS             complement(1232..1993)
                     /locus_tag="VKQ52_18980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ89350.1"
                     /translation="MTDNNDPVILLRNIRKSFGSLEVLKGVDLEVRKGENVVVLGRSG
                     SGKSVLIKIIAGLLSPDTGTVRVLGNDIGQLDTRQLQALRTRIGFSFQSSALYDGMNV
                     RANLEFPLVRNKRHLGRKEIDEAVAQVLDDVGLSRTIGQMPSELSGGQKKRIGIARTL
                     ILQPEIMLYDEPTAGLDPVTSVEINDLINEVQQRYNTTSVIITHDLTCARATGHRIIM
                     LAEGCFLRQGSFDEVFDTDDPRIKKFYNYNFINTA"
     gene            complement(1995..2744)
                     /locus_tag="VKQ52_18985"
     CDS             complement(1995..2744)
                     /locus_tag="VKQ52_18985"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89351.1"
                     /translation="MKAFFLRIYEIYRFTLRFFREAFVPPYEYKEVLNQFFEVGIRSL
                     SIISLTGFITGLVFTKQSRSSLAEFGATSWLPSLMAIAIVRALAPLVTALIIAGKVGS
                     NIGAELGSMKVTEQIEAMEVSSTNPFKFLVVSRILATTCIVPALATYMGFVGMLGAFL
                     NVYRNEQTSFTSFFQAAFVRITFLDIYVLVIKSAIYGFTIGLTGCFNGYYADQGTVGV
                     GRAANTSVVVSMFLIFIEEMIIVQLVNNYLK"
     gene            3254..4048
                     /locus_tag="VKQ52_18990"
     CDS             3254..4048
                     /locus_tag="VKQ52_18990"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HLZ89352.1"
                     /translation="MKMCNPYPTTTAKLFLALALYLASSFRLHASTVYDGVTAIRTTM
                     HYKLSAAAAEARAKRAEMRMIIATAASIYEEMDLEDSGLSRKAFEAAWTGYYKLKKKG
                     LLRRTNILSICDFSQSSSNQRMYVIDVRNRRMLYRTFVAHGINSGEEYANSFSNKMES
                     CKSSLGFYITTGTYSGVNGLSLRINGVDKGFNDNALRRAIVIHGAGYVSQRILRKYGV
                     MGTTFGCPAIPTEMTTQIIPVVKNGSCFFIYYPSKKYLAQSSVLNS"
     gene            4213..5427
                     /locus_tag="VKQ52_18995"
     CDS             4213..5427
                     /locus_tag="VKQ52_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89353.1"
                     /translation="MSISIPSPLPSSPSAARSRVLTGRRAIPLSIYAVSLASLLTMVG
                     ILWDISWHRSIGRDKFLSPPHILVYLGAIFAGLFSGIQVLYTSFFRKEAAKATEVRVW
                     GIFYSPLGSLFCIWGAIAMLTSAPFDDWWHNAYGLDVTILSPPHTLLAMGMIFLQFGA
                     CVGICKYLNADAAEPGAPGGRRSILQTLFVISALSLLTMVYTLFTDYLHIRGMRSGPF
                     YIIATIVSLFLLPAFGRASRMKWGMTAVTGGYFLLVALSNWILQLFPAEPKLGPILTH
                     VTHFQPANFPALVIVPAMAMDLVLQRSKAGDWVRALWLSIAFVGLLVAVQYPLSGFLL
                     ESPNSRNWFFTGNTYYFGLSPNWPWRYRFRPGDIAPLPKLLQGLAVAVLLGWVCARIS
                     LRWGRWMQSIQR"
     gene            5438..7153
                     /locus_tag="VKQ52_19000"
     CDS             5438..7153
                     /locus_tag="VKQ52_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89354.1"
                     /translation="MKKIVWLLALLAGGAGSLSAHVGSPDVSMEGVAGPYRLLVSVRP
                     PDVIPGTAQVTVFIQNGGSVTVSAQPIFFYSGRNGAPSPDLLPAVAGQPGQYKGIVWM
                     MNDGSSSVLLHVSGSLGGGDLVVPIVAVSTAQKKLPAATGYVLAGLGVLLFVLMLTII
                     GSSVADGLARKGEALTAGRRRSKRVAIVVAAVFSSLIVYGGNAWWQSWAARYRRFMYR
                     PMHAAYTLRSDSGVNALTIRINTIDAQRKGWLPYIIPDHGKLMHLFVVRIPGMDAFAH
                     LHPVRLDSATFRTVLPPLPKGRYLAFADIVNLSGFAETLKDTFAIGQDLVDSLHHTDP
                     DDAYAFASPAGAADAGGGGAADAVPGRAADAAGTSREHREMILCGQPGKGVRMKDGST
                     MAAEGAAEGDLEAGQLYDLRFAVLGADGKPAVLEPYLGMMAHAAIIRDDGSTYVHLHP
                     VGTYSVAAQEGLMQRIGEPENEYRLPDARAFSDSIDRLVSRLRGMPEKERDEWLMRKM
                     KMPETATVDTGGSATGPAGMTGMKMSNMVSFPYTFPQPGVYRIWVQVRRNGEVLTAAF
                     DRVVR"
     gene            complement(7165..7896)
                     /locus_tag="VKQ52_19005"
     CDS             complement(7165..7896)
                     /locus_tag="VKQ52_19005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ89355.1"
                     /translation="MINCVIIDDEPLAREGMTSYVREVDFLQLAGVCENPVELTKLLD
                     QHPVDLIFLDIQMPKMNGLDFLKMVQNPPIVIITTAYPTYALDGFQLNVLDYLLKPIT
                     FDRFFKSVNKARDYYQLITRSPQPAADAAADYFFIRCGSKYEKIFFNQILYIEGMQNY
                     ITIYTEKGRYVTLLYLKNLEGNLDSQSFIRVHKSYIVSISKIDAIEGNEIFIQSHRIP
                     ISRNHREEVIQRVVANKLWDKIKFS"
     gene            complement(7911..9020)
                     /locus_tag="VKQ52_19010"
     CDS             complement(7911..9020)
                     /locus_tag="VKQ52_19010"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ89356.1"
                     /translation="MTAGHDTRNWLIRYKLYHLPFWGVYNYFSWVVTVGNPFRATHDL
                     LYLPFTVKYLFYVIVQTLAVYFNLYFLFPRYLEKSKFGVYTILTLLTCVCAAFLIIPG
                     YFASAALAHSTIQQLFGEKACLFNFFSNAFLSTLAAMTLAMSIKLSKNWLESRRRQQL
                     LEKEKLATELNYLRYQFNPHFLFNTINSIFFLIHKNPDMASASLAKFSELLRYQLYEC
                     NDEQIPLSNEITYLQNFIGLEKLRQNNTLGLTIDIQREDTGQVTIAPFLLMTFVENAF
                     KHVSRHTDEANWIKIRLRLTHQQLELSVVNSCSLFESNDILHYSGIGLKNVQRRLDLL
                     YPNQYELSIKNDGSCFEVRLRLQLAAVGLPMQRTA"
     gene            9240..>10349
                     /locus_tag="VKQ52_19015"
     CDS             9240..>10349
                     /locus_tag="VKQ52_19015"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ89357.1"
                     /translation="MKHFLLLLSLLSALSRNGAGQVRGQFVTAGGAPVALADVVVVQL
                     VDSAPVKAGSTDSTGHYRIGPLPPGRYVLRGSNVGYRAWVSPVFVLDQRTRDVDLGRI
                     VLQEEKRELGEVVVRAVKPPVQQSIEGTVVNVQNSLFSKGNSVLEVLERSPGVLLDHR
                     NNSISLNGKSGVTVMIDGKLLHMPEDEILAMLTGMSADNIDKIELLTAPPSRYDASGS
                     AGVINIVLKKNKHQGTTGSFSVNGGYGWGEKGGASSSIDHNTGKTDWYGSYSFLHDHS
                     YSGFVAGGTGLNPALGESGFTFRDSTSWMSNNHNALAGFETKLPRGFTLGASLGYNNS
                     TTSTVTGNLGNYTLPPDSVLVFNGAIHGRSHWDNVI"
BASE COUNT         2068 a         2476 c         3307 g         2498 t
ORIGIN      
        1 gccattgttc ttccgtgccg atatcggggc tgagcaccag ggagtcggtg acatcgtatc
       61 cgtgctggct gcctttcaag gcggtggtga ggggagaggc gtagatcgtg gagataccca
      121 gttcatgaag gtagggcagg agggcttgca ggtcacgaag ggtaaagcga tcattgagtt
      181 ggatccgata ggtacaggaa gggatattca tgtggagcga tttatttttt catttcggcc
      241 gatgagcaaa atattcccca gcgttattcc ttttgttcct tttctctttt gcggaagaat
      301 ccccgcagga ggaagttgtg ttggagggct tcgaggtcgt cactcagctc cttgctgctt
      361 acccgaagat tcttcagggt gcgttgcaga tctgcgcccg cgtcttcgtc atggagcagt
      421 gtgccggcgg gggagttctt gtcgttcagg cccgcgcctg catcgcgaag ggagatagtg
      481 aagtccgtgg cctgggttgc ggcttcattg agccgggcga cggctccctt tatccggttg
      541 aagacggtag tgtcggtggc gagttggctg gcgagaccgt ccggttgttt gagccgggcg
      601 gagaagtcct cgagattggc gatcaggttc tcgctattgg cggccgcggt gcggagatga
      661 tcgatcgaat tgcggaggtg gtcggcgatc gatggatcgc tgatcaattt gccgagggtg
      721 ccctgtccct cgttcagttt tttgctgatg gtcttcaggt tgccggtcat ctccagcaga
      781 ttggcattac ttgcctggag ggtggcgaac atttcctgtg taccggcaag gtgttcgctc
      841 aggaggctgt cattatcgac gatgggtctt gcgttatcgc tgccgccgct gatgacgacg
      901 atcttgttac cgacgagtcc gtcggtgctg atccgggctt tcgcgtcatc ggcgatatgg
      961 ggctgggctt ttttttcgat gttgagggta acggccacct gggacttgtg gtagaagtcg
     1021 atcgatctca ccgtaccgat cttcatcccg gagagccaga cattattccc cggctgcaat
     1081 ccctggacgt catcgaaaag gatgtgtacg cgcaccgatc gtacgaaggc cttgtgctgg
     1141 ccgccgatgg tgaaaatggc tgctatgagg atcaggaccg cgagggtgat gaagacgcct
     1201 acggcgagtc tttttgaaat agctacttcg ttcatgcggt gttgataaag ttataattat
     1261 aaaatttttt tatccgtggg tcgtccgtat cgaacacctc atcgaaactg ccctggcgca
     1321 ggaaacagcc ttcggccagc atgatgatcc gatggccggt ggcccttgcg caggtgaggt
     1381 catgggtaat gatgacggag gtggtgttgt agcgttgctg tacttcattg atgagatcgt
     1441 ttatctcgac gcttgtaacg gggtcgaggc ccgcggtggg ttcgtcatag agcatgatct
     1501 ccggctggag gatcagggta cgggcgatgc cgatgcgttt cttctggccg ccggataact
     1561 cggaaggcat ctggccgatc gtgcgggaga gcccgacatc gtcgaggacc tgggcgacgg
     1621 cttcgtcgat ctccttcctg cccaggtggc gtttgttgcg gacgagcggg aattcgaggt
     1681 tggcgcggac attcattcca tcatagagcg cgctgctctg gaaggaaaag ccgatccggg
     1741 tacgcaacgc ctgcagctgc cgggtatcga gttggccgat atcattgccc aggacgcgga
     1801 cggtgcccgt atcgggtgac aggagaccgg cgatgatctt gatcagtacg gatttgccgc
     1861 tgccgctgcg gccgaggacc acgacattct ctcctttgcg gacttcgagg tcgacgcctt
     1921 tcagcacttc aagggagccg aaggacttcc ggatatttcg caggaggatg acgggatcgt
     1981 tattgtcggt catattattt caaatagttg ttcaccaatt ggacgatgat catctcttcg
     2041 atgaatatca ggaacatgga gaccaccacg gaggtgttgg cggcgcggcc gacgccgacg
     2101 gtgccctggt ctgcatagta gccgttgaaa caacccgtga gcccgatggt aaagccgtag
     2161 atggccgatt ttatcacgag cacgtagatg tcgaggaaag tgatccggac gaaggcggcc
     2221 tggaagaacg aggtgaagct ggtctgttcg ttccggtaaa cgttcaggaa ggcccccagc
     2281 atgccgacga agcccatgta ggtagccagt gccgggacga tacaggtggt ggccaggata
     2341 cggcttacca cgaggaattt gaaggggttg gtggaagata cttccattgc ctcgatctgt
     2401 tcggtcactt tcatcgaacc cagttccgcg ccgatgttgg accctacctt gccggcgatg
     2461 atcagggccg tcacgagggg cgccagggcg cggacgatgg cgatggccat cagggagggg
     2521 agccaggagg tggcgccaaa ctcggccagg gacgaccggg attgttttgt gaacacgagg
     2581 ccggtgataa agccggtaag ggagatgatg gacagtgacc ggatgcctac ttcaaagaac
     2641 tggtttagta cttctttgta ttcatagggt ggaacgaagg cttcccggaa gaagcgaagc
     2701 gtgaatcgat agatctcgta gatgcgtaaa aagaaagcct tcattctttt acagggtaaa
     2761 attatatgcc agtgcggccg ggggcgggat gggaaggaaa ttccccagga cgagtggacg
     2821 gccgcgggaa gggatgccct ggagatgccg gagggacgac cggaccggag ccaggtacga
     2881 gcagtccgag gccgggaata aaaaaagttg ggcgcgggaa atccaaagcg cgccgcttgt
     2941 cgtacattga ctacaattgc gtagttcaaa tgctgaatat cgggcacggg gtgcctttca
     3001 atagtgttat agtcttcata ggcagttagg tttggtaatt cagggggagt cttctccctg
     3061 atttttgttt ttggcacccg ggtaagcgtt tctccttgtc ttttgggtag ttttttggct
     3121 ctaaccgtcc agaaaagaca aaaaagttcc cattccggga caaattctga aattggtacc
     3181 gttttaccga attgaaaaac aatggattgt cttctggtat gggtgttgga cctattattt
     3241 taaatccgtg attatgaaaa tgtgtaaccc ctacccaacg acaaccgcca agctcttcct
     3301 ggctttggcc ctttatcttg cttcttcctt ccggttacac gccagtaccg tttatgatgg
     3361 ggtgacggcc atcaggacca cgatgcatta taagctgtcc gcggctgccg ccgaagcccg
     3421 ggctaagaga gccgagatgc ggatgatcat tgccacggcg gcgagcatct atgaagaaat
     3481 ggacctcgag gattcggggt tgagccggaa ggcgttcgag gctgcctgga ccggttatta
     3541 caagctgaag aaaaaaggcc tcctgcgtag gacgaatatc ctctcgatct gtgatttcag
     3601 ccagtcttcg agcaatcagc ggatgtatgt gatcgatgtc cgcaaccgca ggatgttata
     3661 tcgaactttt gtcgcccatg gcatcaattc cggtgaagag tatgccaact ctttttccaa
     3721 taagatggaa tcctgcaaga gcagcctggg attttatatt acgacgggaa cgtatagcgg
     3781 tgtgaacgga ctttccctgc gcatcaacgg cgtcgataag ggttttaatg acaatgccct
     3841 gaggcgcgcg atcgtgatcc acggggcggg ttatgtaagc cagcgtatcc tgcggaaata
     3901 tggcgtgatg gggaccactt ttggttgccc ggccattccc acggagatga cgacgcagat
     3961 catcccggtg gtgaagaacg gaagttgttt ctttatctac tacccttcga agaagtatct
     4021 ggcccagtct tcggtgttga acagttagcc tttttacccc cggaacatag accgtagccg
     4081 ccctacagag aggggcggcc ccccggcaaa gaatattctt tgccggattt tattatgaaa
     4141 aagccgacgg ctactttcca gcccaaagcc ggcggctttt tttgttatat ttgagtaacc
     4201 gctatcttat ttatgtctat ttccatccct tcaccactgc cttcttcgcc ttcagccgcc
     4261 cggtcgcggg tcctgaccgg caggagagcg atcccgctgt cgatctatgc tgtttctctg
     4321 gcgtcactgt tgacgatggt cggcatcctg tgggatattt cctggcacag aagcatcggc
     4381 cgcgataaat ttctttcgcc gccgcatatc cttgtctacc tgggggccat ttttgccggt
     4441 ttgttctcgg gcatccaggt tttgtatact tcttttttcc ggaaggaggc ggcaaaggcg
     4501 acggaggtcc gggtatgggg tattttctat agtccgctgg gcagtctttt ctgtatctgg
     4561 ggggcgatcg cgatgctgac ctcggcgcct tttgacgact ggtggcataa tgcctatggc
     4621 cttgacgtga ccattctttc gccgccgcat accctgctgg cgatggggat gatctttttg
     4681 cagtttggcg cctgtgtggg catctgcaaa tacctcaatg ccgatgcggc tgagcccggg
     4741 gcgccgggcg ggcggcggtc gatcctgcag accctgtttg tgatcagtgc gctgagcctg
     4801 ctgacgatgg tgtataccct gtttaccgat tacctgcata tccgggggat gcggagcggt
     4861 cctttttata tcattgcgac tatcgtgagc ctgttcctgt tacccgcatt tggccgggcg
     4921 tcgcggatga aatgggggat gacggcggtg acgggcggtt attttctgct ggtggccctt
     4981 tccaactgga tcctgcagct ttttcccgcc gagcccaagc tagggccgat ccttacgcat
     5041 gtcacgcatt ttcaaccggc caattttccc gcgctggtga tcgttccggc gatggctatg
     5101 gacctggtcc tgcagcggag caaggccggc gactgggtca gggcgttgtg gttgagtatc
     5161 gcctttgtgg ggctgctggt ggctgtgcaa tacccgctca gcggcttttt gctggagtca
     5221 ccgaactccc gtaactggtt ctttacgggc aatacctatt attttggcct atcccccaat
     5281 tggccgtggc ggtacaggtt caggccgggg gatatcgcgc cactgcccaa actgttgcag
     5341 ggtctggcgg tggctgtcct gctgggatgg gtctgtgccc ggatcagtct tcgctgggga
     5401 agatggatgc aatctattca acgttaatct tcgacaaatg aaaaaaatag tctggctgct
     5461 ggcattgctg gcgggtggcg ccgggtcatt atccgcgcat gtcggcagcc ccgacgtgtc
     5521 catggaaggg gttgcgggac cctaccgctt gcttgtcagc gtcaggccgc cggatgtgat
     5581 ccccggcacg gcgcaggtga cggtctttat tcagaatggt gggtcggtga cggtgtctgc
     5641 gcaaccgatc tttttttatt ctgggcgcaa cggggcgccg tcgcccgatc tgctgccggc
     5701 ggtggccggg cagcccggtc aatacaaggg tatcgtctgg atgatgaatg acggttcgtc
     5761 cagcgtgctg ctgcatgtca gcggcagtct ggggggtggg gatctcgtcg tgccgatagt
     5821 ggcggtctcg acggcgcaga aaaagctgcc cgcggcgacg ggatatgttt tggcggggct
     5881 tggggtcctt ctctttgtat tgatgctgac gatcatcggg tcgagtgtag ccgatgggct
     5941 tgcccggaaa ggggaggcgc ttacggcggg gcggcggcgg tcgaagcggg tggcgatcgt
     6001 tgtggcggct gttttttctt cgcttatcgt ttatggcggc aatgcctggt ggcaaagctg
     6061 ggcggcgcgg tatcgtcgtt ttatgtaccg gcccatgcat gccgcttata ccctgcggtc
     6121 ggacagcggg gtgaatgcgc tgacgatccg gatcaacacg atcgatgcgc agcggaaggg
     6181 gtggttgccg tatatcatac ccgatcatgg taagctgatg catctttttg tcgtccggat
     6241 cccggggatg gatgcttttg cccatctgca cccggtgaga ctcgactccg ctactttccg
     6301 gaccgtgctg ccgcccttgc caaaggggcg gtatctggca tttgccgata ttgtcaatct
     6361 ctcgggtttt gccgagaccc tgaaggacac gtttgcaatc ggccaggacc tcgtcgacag
     6421 cctgcatcat acggaccccg atgatgccta tgcctttgcg tcgcccgccg gcgcggctga
     6481 tgcgggtggg ggcggcgcgg ctgatgcggt tcctgggcgc gcggctgatg cggccggcac
     6541 gtcgcgggag caccgggaga tgatcctgtg tggacagccc ggcaaggggg taagaatgaa
     6601 ggatgggtcg acgatggcgg cggaaggcgc cgcggaggga gacctggaag cgggtcaact
     6661 atatgatctg cggtttgcgg tgctgggtgc cgatgggaaa cccgccgttc tcgagcctta
     6721 cctggggatg atggcgcatg ccgcgatcat cagggacgat ggcagcacgt atgtgcatct
     6781 gcacccggtg ggcacatact cggtggcggc gcaggagggg cttatgcagc ggatcggcga
     6841 gccggagaat gaataccggc tgcccgacgc cagggccttt agcgatagta tcgacaggct
     6901 tgtttcgcgg ctgcggggga tgccggagaa ggagagggat gagtggctga tgcggaagat
     6961 gaagatgccg gagactgcga cggtcgatac gggcgggtcg gctacgggtc ccgcgggcat
     7021 gacggggatg aagatgagca atatggtcag tttcccgtat accttcccgc agcccggtgt
     7081 ttatcgcata tgggtgcagg tgaggaggaa cggcgaggta ttgaccgccg ccttcgacag
     7141 ggtcgtgaga taggctgacg cgcgtcagga gaatttgatc ttgtcccaga gtttgttggc
     7201 gacgacgcgt tggatgacct cttcccggtg gtttcggctg atagggatgc gatgggattg
     7261 gataaagatc tcattgcctt cgatggcgtc gatcttactg atagaaacga tataggattt
     7321 gtgtacgcgg ataaaggatt ggctgtcgag gttcccttca aggtttttga gatagagcag
     7381 ggtgacatac ctgccctttt cggtgtagat cgtgatatag ttctgcatcc cttcgatata
     7441 gaggatctgg ttgaaaaaga tcttctcgta tttgctgccg cagcggataa agaaataatc
     7501 ggcggcggca tcggcggctg gctgggggct gcgggtaatg agctggtaat agtccctggc
     7561 tttgttgacg gatttgaaaa accggtcgaa ggtgatgggt ttcagcagat agtcgagtac
     7621 gttcagctgg aatccatcga gcgcgtaggt aggataggcg gtagtgatga tgacgatggg
     7681 cgggttttgc accatcttga gaaagtccag gccgttcatt ttcggcatct ggatgtcgag
     7741 gaagatcagg tcgacgggat gctggtccag cagttttgtc agttcgaccg ggttctcgca
     7801 aacgccggcg agctgcagga aatccacttc gcgcacatag cttgtcattc cctcccgggc
     7861 cagtggttca tcatcgataa tcacgcaatt gatcattctt ttcattttta tcaggcggtt
     7921 ctttgcatgg gcagacccac tgcggccagt tgcagccgga gcctgacctc gaagcagctg
     7981 ccgtcatttt ttatagacag ttcgtattga ttcgggtata gcaggtccag tcttcgctgg
     8041 acgttcttca acccgatgcc gctgtaatga aggatgtcat tggattcgaa cagggagcag
     8101 ctgttgacga cggacagttc cagttgctga tgggtcaggc gcagccggat ctttatccag
     8161 ttggcctcat cggtgtgacg ggagacatgt ttaaaggcgt tctcgacgaa ggtcatgagc
     8221 aggaaggggg cgatcgtcac ctgcccggta tcctcgcgct ggatatcgat ggtcaggccc
     8281 aatgtgttgt tttggcggag tttttccaga ccgataaaat tctgcagata agtgatctcg
     8341 ttgctcagcg ggatctgctc gtcattgcat tcgtataact ggtagcgcaa cagttcggag
     8401 aatttggcca gcgatgcgga ggccatatcg gggttcttat ggatcaggaa gaagatggaa
     8461 ttgatggtat tgaacaggaa atgcgggttg aactggtagc ggagatagtt cagctccgtc
     8521 gccagtttct ctttttcgag cagttgctgg cgccgccggg attcgagcca gttctttgag
     8581 agcttgatgc tcatggccag ggtcatggcg gcgagtgtcg agagaaaggc gttgctgaag
     8641 aaattgaaga gacaggcttt ttcgccaaac agctgctgaa tggtcgaatg ggccagcgcg
     8701 gcgctggcaa aatagcccgg gatgataagg aaggcggcgc agacgcaggt gagcagggtt
     8761 aagatagtgt ataccccgaa tttactcttc tcgaggtagc gggggaagag gaaatagagg
     8821 ttgaaataga cggcgagggt ttgtacgatg acgtaaaaga gatacttgac ggtgaacggg
     8881 aggtagagca gatcatgtgt cgccctaaag gggttgccga cggttaccac ccaggaaaaa
     8941 tagttataga caccccagaa gggaaggtgg tacagcttat agcggataag ccagttgcgg
     9001 gtgtcgtgac cagccgtcat gcaactaaga taccccgaaa tccaggaatt tttccggcta
     9061 ctatatgaat gacaccaaat aaatgacgag ctgcagcccc gtctccataa ccggaggatc
     9121 gggaggtttt tttctgtttt atcgggaatc ccggtaattt tttgcgcttc gtcaatttat
     9181 gatcgccggt ggtcatacgc attttttccc gccttccgcc gcgccttatt ttgtcctcca
     9241 tgaagcattt ccttttactc ttatccctgc tgtccgccct gtcccggaat ggcgccgggc
     9301 aggtgcgcgg gcagtttgtg acggcgggtg gcgcaccggt ggcgcttgcc gatgtggtgg
     9361 tagtacagtt ggtggactcg gcgcctgtga aagccgggtc gacggattcc accgggcatt
     9421 accggatcgg gccgttgccg ccggggcggt atgtcctgcg aggcagcaat gtcggctacc
     9481 gggcatgggt atcgcctgtg ttcgtactgg accaacggac acgggatgtc gatctgggca
     9541 ggatcgtcct gcaggaggaa aagcgggagt tgggcgaggt tgttgtgcgc gccgtgaagc
     9601 cacccgtgca acaaagtatc gagggaactg tggtcaatgt gcagaacagt ctcttcagca
     9661 agggcaattc ggtgctggag gtgctggagc gcagtcccgg ggtgttgctg gatcaccgga
     9721 acaacagcat cagtctgaac ggcaagtcgg gggtgacggt tatgatcgac gggaaactgc
     9781 tgcatatgcc ggaggacgag atcctggcca tgctcaccgg gatgagtgca gacaatatcg
     9841 acaagatcga attgctgacg gcgccgccat cgcggtatga tgcgtcgggt agcgccgggg
     9901 tgatcaatat cgtgttaaag aagaacaagc atcaggggac gacgggttcg ttctctgtga
     9961 acggtggtta tggctggggg gagaagggtg gggcaagcag tagtatcgat cataacaccg
    10021 gcaagaccga ttggtatggg tcatattcgt tcctgcacga tcatagttat agcggtttcg
    10081 tggccggggg gacgggcctc aacccggcat tgggggaatc cgggtttact ttccgggact
    10141 ccacgtcctg gatgagcaac aatcacaatg cgctcgcggg ttttgaaacc aaattgccgc
    10201 gggggttcac gcttggcgcc agtcttgggt acaacaacag tacgacaagc accgtcacgg
    10261 gcaacctggg aaattatacc cttcccccag acagcgtgct tgtctttaac ggggcgatcc
    10321 atggcaggag tcattgggat aatgtgatc
//
LOCUS       DATGFG010001002         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_409979, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001002 DATGFG010000000
VERSION     DATGFG010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409979"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VKQ52_19020"
     CDS             <1..483
                     /locus_tag="VKQ52_19020"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HLZ89358.1"
                     /translation="DFWNEEKQAFMQFPGSETVDAASLLLPLFRFISPKDPRWLSTLK
                     RIEDELVTDSLVYRYRPGIAAPDGFISKEGTFSMCSFWYVECLSRSGQLDKARFFFEK
                     MLGYANHLGLFSEQLGFEGEHLGNFPQAFTHLGLISAALNLDQQFNDNRNKNINLDEI
                     "
     gene            487..1791
                     /locus_tag="VKQ52_19025"
     CDS             487..1791
                     /locus_tag="VKQ52_19025"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HLZ89359.1"
                     /translation="MASTTRPARKTRAAKDRNPSRLQRLLKTLGPGIVTGASDDDPSG
                     IVTYSQAGSQFGLATLWTALLTFPLMAAMQGMAARIGLVTSHGLTGTLRMYYPKWLLF
                     FVLLFCFPAVILNIGADIQSMGAVTNLLIPAVPAFAASIFFTALLLFVIIRYPYQKMA
                     GILKWLCLGLLLYLIVPFLVRQNWREVAHRTFVPEIRLDKDFLGILVAILGTTISPYL
                     FFWQATMEVEDQQHDTRKRLVMVDKKALDDMRSDVNLGMFFSNLVMFFVILTAGSVLF
                     PAGIKNIDTVEQAAQALKPLAGNLSYVLFAVGVIGTGLLAIPVLAGSLSYMLAETLQF
                     SMGLDKKFNEARRFYAVIIVSLIAGLCMQFSGISPVKALLYTAILYGLTSPVLIAVIL
                     HIGNNKRIMGEHTNTRFSNLLGFLTLILMTTAGVALVWFTFA"
     gene            1839..>2078
                     /locus_tag="VKQ52_19030"
     CDS             1839..>2078
                     /locus_tag="VKQ52_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89360.1"
                     /translation="MEKITTPDTMTTSSQMLEKLRIAHMDNEFRWTPNGFTTTNGKTY
                     RPGDLEIVRTLRFEGESDPSDMEILYIIKTNDGLIG"
BASE COUNT          459 a          646 c          505 g          468 t
ORIGIN      
        1 gatttctgga acgaagagaa acaggccttc atgcaattcc cgggttcgga gaccgttgac
       61 gcagctagcc tgctgctgcc cctgtttcgc ttcatcagtc caaaagaccc ccgctggtta
      121 tcgacactga agaggatcga ggacgaactg gtaaccgact cgctggtcta tcgttatcgc
      181 cccgggatcg ccgccccgga tggctttatc agcaaagaag gcactttctc gatgtgcagc
      241 ttctggtacg tggagtgctt gtcccgatcg ggtcagttgg ataaagcccg gttcttcttt
      301 gaaaagatgc tgggatatgc caatcacctg ggcctctttt ccgaacaact cggtttcgag
      361 ggtgaacacc tggggaattt cccccaggcc tttacacatc tggggttgat cagcgccgcg
      421 ctgaacctcg atcaacagtt caatgataat cgcaataaga acattaacct ggacgaaatc
      481 taattaatgg catccaccac cagacccgcc cggaagaccc gcgccgcaaa agacaggaac
      541 ccttccaggc tccaacgact gctaaaaacc ctgggacccg gtattgtcac cggcgccagc
      601 gatgacgacc cttccggcat cgtcacctat tcccaggccg gttcacagtt tggtttggcg
      661 acactctgga cggccctgct caccttcccc ctgatggccg cgatgcaggg catggcagcc
      721 aggatcggac tggtcacctc ccacggcctc acaggaaccc tgcggatgta ctatccaaaa
      781 tggctgctct tcttcgtgct ccttttttgc ttcccggccg tgatcctcaa catcggcgcc
      841 gatatccaaa gcatgggcgc cgtaaccaac ctgctgatcc ccgccgtacc cgcattcgcg
      901 gcaagcatct tcttcaccgc cctgctcctg ttcgtgatca tccgctatcc ctaccaaaag
      961 atggccggta ttctcaaatg gctctgtctc ggcctgttgc tctatcttat cgtccctttt
     1021 ctcgttcgtc agaactggag agaagtcgcg caccgcacct tcgtgccgga gatccgtctc
     1081 gacaaagact tccttggcat cctggtcgcc atcctcggaa ccaccatctc cccctacctc
     1141 tttttctggc aggcgacgat ggaggtagag gaccagcagc acgatacccg gaaaaggctg
     1201 gtgatggtag acaaaaaagc gctcgatgac atgcgcagcg acgtgaacct gggtatgttc
     1261 ttctccaacc tggtcatgtt cttcgtgatc ctgacggccg ggagcgtatt gttccccgcc
     1321 gggataaaga atatcgatac ggtcgaacag gccgctcagg cgttaaaacc cctggccggg
     1381 aatctaagct atgtgctgtt cgccgtagga gtgatcggca ccggcctcct cgccatcccc
     1441 gtgctcgccg gcagcctttc ctatatgctg gcggaaaccc ttcaattctc catgggattg
     1501 gacaagaaat tcaacgaggc ccggcgtttc tatgcggtca tcatcgtaag cctcattgcc
     1561 ggtctctgca tgcagttctc cgggatctcg ccggtgaaag cgctcctgta taccgcgatc
     1621 ctctatggtc ttacttcccc ggtgctgatc gcggtgatcc tgcacatcgg caacaacaaa
     1681 aggatcatgg gggaacatac caatacccgg ttctccaacc tcctcggctt cctgaccctc
     1741 atcctgatga ccactgcggg tgtcgcgctc gtctggttca ctttcgcctg atccgccggg
     1801 tacgcaattt gaaaacgaac gtttaaatac tcatcatcat ggaaaaaata acgacccccg
     1861 acaccatgac cacttcctcc cagatgctgg aaaaacttcg catcgcccat atggacaatg
     1921 aattccgctg gacacccaac gggtttacga caaccaacgg caaaacctat cggcccgggg
     1981 acctcgagat cgtccgtacc ctccgcttcg aaggggaaag cgacccttca gatatggaaa
     2041 tattatatat cataaagacc aacgacgggc tcatcggc
//
LOCUS       DATGFG010001003         4725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1704285, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001003 DATGFG010000000
VERSION     DATGFG010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704285"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..843)
                     /locus_tag="VKQ52_19035"
     CDS             complement(154..843)
                     /locus_tag="VKQ52_19035"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLZ89361.1"
                     /translation="MLQTVTSAFFLGLLSSLHCVGMCGPLLLALPNQRLGPAGRAFSS
                     FFYHGGRILTYTLGGLVFGLAGHHLYLAGWQQGLSIALGAAILMLYIIRRLPAAPSLK
                     LPPRLMRRIGGFWQSPTLPGFFLLGVANGLLPCGMVYLAIAAALLQHTVTQAILFMAF
                     FGAGTLPLLLGTQYLGRKITANWRIQLRRTLPIVTVLMAVLLILRGLNLGIPFISPVL
                     AAAPKHPISCH"
     gene            complement(856..1293)
                     /locus_tag="VKQ52_19040"
     CDS             complement(856..1293)
                     /locus_tag="VKQ52_19040"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HLZ89362.1"
                     /translation="MNWGNKLLLVFVVFGGGISYMVFRCMQTPVDLVSKDYYRDELSY
                     QQVIDGASKANALSEDIRIGNTPTGIRLELPAEMKHRSVTGSIRFYCAADAGRDREIT
                     LDPDGEGGQDIPRSALIPGRYTVSISWRSAGVGYFSQQPYVIH"
     gene            complement(1312..2733)
                     /gene="ccoG"
                     /locus_tag="VKQ52_19045"
     CDS             complement(1312..2733)
                     /gene="ccoG"
                     /locus_tag="VKQ52_19045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HLZ89363.1"
                     /translation="MDTESPTLLTADQSFRDHLATVDAKGRRKWVYAQKPHGKWYNLR
                     TYISWGFFLLFFSLPFVYVNGRPLFLFNIPNARFILFGKVFWPQDFFIFGLTMVTFII
                     FVILFTAAFGRLFCGWVCPQTIFMEMLFRKVEYAILGDAAAQRLARSRPRTVNWMLRS
                     SAKHLVFFLLSFIIANTFLSYIIGSRELGRIITEPLSSHLGTLSALLIFSGVFYAVYA
                     FFREQACTVVCPYGRLQSVLLDRNSMIVAYDYKRGEPRARFAKQKDSSKGDCLDCNQC
                     VKVCPTGIDIRNGTQMECVGCTACIDACNVMMEKTGRPPDLIRYASENGISNHQPLQY
                     TLRMKLYTGLLTVLVMILAGLLLSRKEVDATIMRTPGMLYQERGTDSVSNLYTIKVAN
                     KTVRRVPLQLRLEGETGAIQVIGGNEIPIKKEEEGSGNFFVVLPRRDILSRKTSIRIG
                     LYDNNKKIDEIRTNFLGPQPESN"
     gene            complement(2735..3757)
                     /locus_tag="VKQ52_19050"
     CDS             complement(2735..3757)
                     /locus_tag="VKQ52_19050"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HLZ89364.1"
                     /translation="MAQSTDNPFVVIMLIIIIILGIAITLLANVVLVAAQFRAAEPAT
                     DDAEPSPDTATAPSPAPTSTRNDERKPPVLAGAGAALAIPAVNGISDTAFYLIVSVVA
                     LEIFIIMALLYNLRVIIGRTKAAAVKQNSQAVPATPRISWWDRLNRFRPITQEADLDL
                     GHDYDGIRELDNRLPPWWLYGFYCTILFACVYLWRYHVSHTAPLPAEEYRIAVLNAEA
                     QKADYLKKAANNVDENTVKLLSTPTDLAAGRTVFETTCFACHGKHGEGGVGPNLTDDY
                     WLHGGSVNDVFKTIKYGYPDKGMKSWKDDYSPVQIAQIASYVRSLRGSKPDNPKAPQG
                     NLYKEQ"
     gene            complement(3790..3948)
                     /locus_tag="VKQ52_19055"
     CDS             complement(3790..3948)
                     /locus_tag="VKQ52_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89365.1"
                     /translation="MKFINYLEKISGVSIYGLASLVLFGTIFLVMLFWTIKADKKMIE
                     EIRQLPLN"
     gene            complement(3961..>4725)
                     /gene="ccoO"
                     /locus_tag="VKQ52_19060"
     CDS             complement(3961..>4725)
                     /gene="ccoO"
                     /locus_tag="VKQ52_19060"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HLZ89366.1"
                     /translation="RRRARRASAGGVSVMSIWARHQALEKNSILLVIGILLVIAIGGI
                     VEVAPLFYLKSTVEKVEGMRPYTPLELAGRNIYVREGCYLCHSQMVRPFRDEVARYGE
                     YSKAGEFVYDHPFQWGSKRTGPDLARVGGKYPDSWHYNHMLDPSSMSPGSIMPSYVWL
                     FDHEIDTVHTYKMIKAMQTLGVPYPDGYADQANKDLTAQARSIRASLKTDKLSIGENK
                     EIVALIAYLQRLGKDIKAAPKNQTAGVGDGRAIMGN"
BASE COUNT          931 a         1134 c         1631 g         1029 t
ORIGIN      
        1 ttcgcgatcg ggatgttctt ttgttcgccg gtgttcgtgg cgtggatggc ctatgtggtc
       61 atccggtttg ggaagcacgg tgaaaaggaa ttggcggagg gggaggagtt cggttatggg
      121 gaccgcagcc gcgagacgct gggtgtctgg tgatcaatga caggagatgg ggtgtttcgg
      181 ggcggcggcg agtacggggc tgatgaaagg gatgccgagg ttgagaccac ggaggatcag
      241 caggacggcc atgaggacgg tgacgatcgg gagggtgcgg cggagttgga tgcgccaatt
      301 ggccgtgatc ttcctgccca ggtattgtgt tcccagcagg aggggcaggg tgcccgcgcc
      361 aaagaaggcc atgaacagga tcgcctgggt aacggtatgc tgcaagaggg cggcggcgat
      421 ggccaggtag accattccac aggggagcag gccgttggcg acacccagca ggaagaagcc
      481 gggcagcgtg ggcgattgcc agaagccacc gatccggcgc atcagccgcg gtgggagttt
      541 gagtgaagga gctgcgggga gacggcggat gatgtagagc atcaggatcg ccgcacccag
      601 ggcaatggat agcccttgtt gccagccggc gaggtagagg tgatggccgg cgagtccaaa
      661 gaccaggccg cccagggtat aggtgaggat acgaccgccg tgatagaaga aggaggagaa
      721 ggcgcgaccc gcgggaccga gccgttggtt gggaagggcg agcaggaggg gaccgcacat
      781 gcccacgcag tgaaggctgc tcagcaggcc gagaaaaaac gcggatgtga cggtttgcag
      841 catgggatcc gggtatcagt ggatgacata gggttgctgg gagaaataac cgacccccgc
      901 cgagcgccaa ctgatgctga cggtataacg gccggggatg agtgccgacc ttgggatatc
      961 ctggccgcct tccccgtcgg ggtccagggt gatctcccgg tcacggccgg cgtcggcggc
     1021 gcaatagaac cggatgctgc cggtgacgga ccggtgtttc atttccgcgg gcagttccag
     1081 ccggatgccc gtgggcgtgt tcccgatgcg gatgtcctct gaaagggcat tggccttact
     1141 ggcgccgtcg atcacctgct ggtaggacag ttcatcgcga taataatctt tcgataccag
     1201 gtcgaccggg gtctgcatac agcggaagac catataggag atgccgccgc cgaagacgac
     1261 gaagacgagt aagagtttat ttccccaatt catatgtatt gcttttgatg gttaattgga
     1321 ttccggctgc gggccgagaa aattcgttcg gatctcgtcg atctttttat tgttatcata
     1381 gagcccgatg cggatggagg tcttgcggga aaggatgtcg cggcgcggca ggacgacgaa
     1441 gaaattaccc gaaccttcct cttctttttt gatggggatc tcgttgccgc cgatgacctg
     1501 gatcgcgccg gtctcgcctt cgagccgcaa ttggagcggt acccggcgga cggtcttgtt
     1561 ggcgaccttg atggtataga ggttggacac gctgtcggtt ccgcgctcct ggtagagcat
     1621 accgggggtg cgcatgatgg tggcatccac ttccttgcgg ctgagcagca gaccagcgag
     1681 gatcattacc aggacggtga gcaggccggt gtacagtttc atccgcaggg tatactgcag
     1741 gggctgatgg ttgctgatgc cgttctcgga tgcgtagcgg atgaggtcgg gcggccggcc
     1801 ggtcttttcc atcatgacgt tacaggcatc gatacaggcg gtgcagccga cgcattccat
     1861 ctgggtgcca ttgcggatat cgataccggt ggggcagacc tttacgcatt ggttgcagtc
     1921 caggcagtct cctttggagg agtctttctg ttttgcgaat ctggcgcgtg gttcccctct
     1981 cttatagtca tatgcgacga tcatcgagtt gcggtcgagc aggacgcttt gcagccggcc
     2041 gtaggggcag acgacggtac aggcctgctc gcgaaagaag gcatagacgg cgtagaagac
     2101 gccgctgaag atcagcaacg ccgacagggt gcccaggtgg ctgctcaggg gttcggtgat
     2161 gatccggccc aattcccggg agccgatgat ataggacagg aaggtattgg cgatgatgaa
     2221 ggaaagcagg aagaagacca ggtgtttggc cgatgaccgg agcatccagt ttaccgttcg
     2281 gggccggctg cgggcgaggc gttgggcggc ggcgtcacct aggatggcat attccacttt
     2341 gcggaagagc atttccatga aaatggtttg cgggcatacc cagccgcaga agaggcggcc
     2401 gaaggcggcg gtgaacagga tgacaaagat gatgaaggtg accatcgtga ggccgaagat
     2461 gaagaaatcc tgtggccaga acaccttgcc gaaaaggata aagcgggcgt tgggaatatt
     2521 gaacaggaac aggggcctgc cgttgacata gacgaagggc agggaaaaga agaggagaaa
     2581 gaagccccag cttatatagg tgcggaggtt gtaccatttc ccatggggtt tttgtgcgta
     2641 tacccatttc cgacggcctt ttgcgtcgac ggtggccagg tgatcgcgaa aagactggtc
     2701 ggcagtaagg agtgtagggc tttcggtatc catgtcattg ttctttgtag aggttccctt
     2761 gcggggcctt cgggttatcg ggtttgctgc cgcgcaggga tcttacgtag gaggctatct
     2821 gggcgatctg cacgggcgaa tagtcgtcct tccagctttt cattcccttg tcggggtagc
     2881 cgtatttgat ggtcttgaat acgtcgttga cgctgccgcc atgcagccag tagtcatcgg
     2941 tgaggttggg tcctaccccg ccctcgccgt gtttgccgtg gcaggcgaag caggtggttt
     3001 cgaagaccgt gcggcctgcg gcgagatcgg tgggagtgga taggagtttt accgtgttct
     3061 cgtcgacatt gttcgcggcc tttttcaggt aatcggcctt ttgggcttcc gcattgagta
     3121 cggcgatgcg gtattcctcg gcgggtagcg gcgcggtatg ggaaacatga tagcgccaga
     3181 gatatacgca ggcaaagagg atggtgcaat agaatccgta gagccaccag ggcgggaggc
     3241 ggttatccag ttcgcggatg ccgtcgtagt catggccaag gtcgaggtcg gcctcctggg
     3301 tgatcggccg gaagcggttg agcctgtccc accaggagat gcgcggggtc gcgggtacgg
     3361 cctggctatt ttgtttcacg gcggcggctt ttgtcctgcc gatgatgacg cggaggttat
     3421 acaaaagggc catgatgatg aagatctcga gggcgacaac gcttacaata aggtagaagg
     3481 cggtgtcgga gatgccgttg acggcgggta tggccagggc tgcgccggcg cctgccaata
     3541 ccggaggttt gcgttcatcg tttcttgttg acgtgggtgc gggtgacggt gcggttgccg
     3601 tatcgggcga gggttccgca tcgtcggtcg ccggttcggc tgcccggaat tgtgcagcaa
     3661 cgagtacgac gttggccaac agggtgatgg caatccccag gatgatgatg atgatcagca
     3721 tgatgacgac gaaggggttg tcggtggact gtgccataaa ttcgattttt atgatagttt
     3781 ttgggtttgt tagttaaggg gcaattgccg gatctcttcg atcatttttt tgtccgcctt
     3841 tatggtccag aacagcatga ccaggaagat cgttccgaag agtaccaggg aggcgaggcc
     3901 gtagatgctg acgccgctga tcttttcgag gtagttgatg aatttcatgt gttgtttttt
     3961 ttagtttccc ataatggccc ggccgtcgcc cacgccggcg gtttggttct ttggcgctgc
     4021 cttgatgtcc ttacccagcc gttggagata cgcgatgagg gcgacgatct ccttgttctc
     4081 gccgatggat agcttatctg tcttcaggga ggcccttatg gaccgtgcct gcgcggtgag
     4141 gtctttattc gcctggtcgg catatccatc gggatagggt acgccgaggg tttgcatcgc
     4201 ctttatcatc ttataggtat ggacggtatc gatctcgtgg tcgaagagcc agacatagga
     4261 cggcatgatc gagccgggtg acatggagga ggggtccagc atgtggttat agtgccagct
     4321 gtcgggatat ttccccccta ccctggccag atcgggcccg gtccttttgg agccccactg
     4381 gaaggggtgg tcgtagacga attcgccggc cttggaatat tccccgtagc gggctacttc
     4441 gtcgcggaag gggcggacca tctgggaatg gcagagatag cagccttcgc ggacatagat
     4501 gttgcgtccg gcgagctcga gcggcgtata ggggcgcatg ccctcgacct tctcgaccgt
     4561 gctcttgagg tagaacagcg gcgccacctc tacgatgccg ccgatggcaa tgaccaggag
     4621 aatgccgatc acgagcagga tcgagttctt ctcaagagct tggtgccttg cccatatcga
     4681 catgacgctt actccgccgg ctgaagcacg acgggcgcgg cggcg
//
LOCUS       DATGFG010001004         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_398092, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001004 DATGFG010000000
VERSION     DATGFG010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398092"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VKQ52_19065"
     CDS             complement(<1..518)
                     /locus_tag="VKQ52_19065"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLZ89367.1"
                     /translation="MDIAPDGVILSTCARFAALLKRRNIHDFLGSSLLDIFPRLGKTD
                     PVLTPDLLENGLPPYIDLSVKGQSSKPVTIRWIPTPKYALDFQGWQLTGPRLYADPFP
                     ESGDRRRTTSEDMPVPANLIKQVSDIIITTDLEDRIVYWNIAAEKFYDIPAPLAIGQS
                     LRELLHYDYLNTT"
     gene            complement(690..2069)
                     /locus_tag="VKQ52_19070"
     CDS             complement(690..2069)
                     /locus_tag="VKQ52_19070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLZ89368.1"
                     /translation="MLRKLVLPILLLSVALSGFAQEKVVADKIAGIVGDKIILRSELV
                     TAIQDMIRNNGGQEVKGVDECTILDNMLIQKALVLQAEKDYLPVTDEEVEAEVDQKIR
                     YFINLYGGKDAFEQIAQRTVYQAKMDFMAPIREQRLADAMRKKVVQDITVTPTEVKEY
                     FDKIPDDKRIFYESEMQLDQIVIYPKASRDIEKLTIDELNEYKQQVESGQRKFDFLAR
                     LYSQDPGTKEKGGIIELSRADSKTFDPVFFSAAFRLKEGQVSPVIKSKYGYHIIQMVS
                     RNGDDAVVRHILRIPEITQAEIEETTKQLDTIRTALVNGTATFGEAVARFGDETQKST
                     AGQVLNPRTGSTFLTISDLDKEMVLLLKDANLKPGEISKPSAFADERGKKGVRIVKML
                     SKSEPHRENLRDDYDRIAARALDQKRTSALEKWFVTKIPTFYIMIDGEYRNCANLAKW
                     QISSATAGN"
     gene            complement(2131..>2800)
                     /locus_tag="VKQ52_19075"
     CDS             complement(2131..>2800)
                     /locus_tag="VKQ52_19075"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89369.1"
                     /translation="TLDEIGRRLALGNFKELNLIIKGDVDGSVEALSDSLQKLSTEEI
                     AVRVVHKAVGAITESDVLLATASDAVIVGFNVRPSSQASRLAENEGVEIKTYSIIYNA
                     IEEVKSAMEGMLEPKIQEKIVANVEVRNVFKFDKATVAGCYVLDGKIFRNSKIRLVRD
                     GIVIYPVGEGATAELASLKRFKDDVKEVPSSMECGLTIKNYNDIRVGDIVEAYEEEEV
                     KRTL"
BASE COUNT          557 a          646 c          832 g          765 t
ORIGIN      
        1 gtcgtattta aataatcgta atgtaggagt tcgcggaggg attggccgat ggccaggggc
       61 gccgggatat cgtagaactt ctctgccgcg atattccagt agacgatgcg gtcctcgagg
      121 tccgtggtaa tgatgatatc cgaaacctgt ttgatcaggt tggccgggac gggcatgtct
      181 tcggaggtgg tacgccggcg gtcgccggat tcggggaagg gatcggcgta gagtcttggg
      241 cctgtcagtt gccagccctg aaaatcgagc gcatattttg gtgtggggat ccagcggatg
      301 gtaacgggtt tggaactctg ccctttcacg gaaagatcga tatagggggg aaggccgttc
      361 tccagcaggt cgggtgtgag gacggggtcg gtttttccca ggcggggaaa gatgtccagg
      421 aggcttgatc ctaaaaaatc atggatattt cgtcttttga ggagggctgc aaagcgggcg
      481 caagtggata aaatgactcc atcgggagct atatccataa aataaggttg tctaaactgc
      541 tttgtcgtag gcacggactt ttgggcgcta cttagcatac aaaggaaaag aattgagcta
      601 tcaaattact aaagaagccc gtatggccta tgaaataata tcttatttcg ggtccactaa
      661 gtgtaaatat ctaaaaatat tagcggctgt taattaccgg ctgttgcaga gctgatctgc
      721 catttggcga gattggcgca gttgcgatat tccccgtcga tcatgatgta gaaggtgggg
      781 atctttgtca caaaccattt ctccaatgcc gaggtacgtt tttgatcgag ggcccttgcg
      841 gcgatgcggt catagtcgtc ccggagattt tcgcggtgtg gttctgattt ggagagcatt
      901 tttacgatgc ggacgccttt ctttccgcgt tcatctgcga aggcggaggg tttggagatc
      961 tcaccgggtt tcaggttggc atctttcagc aggaggacca tctccttgtc gaggtcggaa
     1021 atggtgagga aggtgctgcc tgtccttggg ttcagtacct ggcccgcggt ggacttctgt
     1081 gtctcgtcgc cgaaacgggc aacggcttcg ccaaaggtgg cggttccatt gacgagggct
     1141 gtgcggatgg tatcgagttg tttggtggtc tcctcgatct cggcctgggt gatctcgggt
     1201 attcggagga tatgtcttac gacggcgtcg tcaccgttgc ggcttaccat ctggatgatg
     1261 tggtagccat acttgctctt tatgacagga gatacctggc cttctttcag gcggaaggcg
     1321 gcggagaaga atacggggtc gaaggtcttg ctgtcggcgc ggctcaattc gatgatgccg
     1381 cctttttctt tggttccggg gtcctgcgaa tacaagcggg cgaggaagtc gaatttcctc
     1441 tggccggatt ccacctgttg tttgtattcg ttgagttcgt ctatagtaag tttctcgata
     1501 tcgcggcttg cttttggata gatgacgatc tgatccaact gcatttcgga ttcgtagaag
     1561 atgcgtttat catcggggat cttgtcgaag tattctttta cttcggtagg cgtgaccgtg
     1621 atatcctgga ccactttttt gcgcatggcg tcggcgagcc gttgttcgcg gatgggggcc
     1681 atgaagtcca tcttggcctg gtataccgta cgctgggcga tctgttcgaa ggcatcctta
     1741 cctccataga ggttgataaa ataccggatc ttctggtcta cttccgcttc tacttcttcg
     1801 tcggtgacgg gcaggtagtc tttttccgcc tgcaatacca gcgctttttg gatcagcatg
     1861 ttatccagaa tagtgcattc gtcgacccct tttacttcct gtccgccgtt attccttatc
     1921 atgtcctgga tggcggtgac cagctccgat ctgaggatga tcttatcgcc aacgataccg
     1981 gcgatcttat ctgcgactac cttctcctgg gcaaagccgg ataaagcgac agaaagcagt
     2041 agtatgggga ggacgagttt gcgtaacatg catcaaattt aaaatggata aaatgttaaa
     2101 tacctccgaa aggaggcatt taacaaaata ttacagcgtg cgctttactt cttcttcttc
     2161 gtatgcttcg acgatgtcgc ctacgcggat gtcgttatag ttcttgatgg tgaggccgca
     2221 ttccatgctt gacgggactt ctttcacgtc gtccttgaag cgtttgagtg aagccagttc
     2281 ggcggtggcg ccttccccta cggggtagat cacgatgccg tcgcgtacga ggcggatctt
     2341 ggagttgcgg aagatctttc catcgagtac atagcaaccg gcgacggttg ccttgtcgaa
     2401 tttgaagacg ttgcggactt ccacgttggc cacgatcttc tcctggatct ttggttccag
     2461 catcccttcc atggcgctct tgacctcttc gatcgcgttg tagatgatgg agtaggtctt
     2521 gatctccacg ccttcgttct ccgcgaggcg ggaagcctgg gaagaggggc ggacgttgaa
     2581 gcccacgata acggcgtcgg aggcggttgc cagcaggaca tcgctttcgg tgatggcgcc
     2641 cacggccttg tgtacgacgc ggacggcgat ctcttccgtc gacagtttct gcagcgagtc
     2701 gctgagggct tccacggagc cgtccacgtc acccttgatg atgaggttga gctctttgaa
     2761 gttacccagt gcgaggcggc ggccgatctc gtcgagggtg
//
LOCUS       DATGFG010001005         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_398169, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001005 DATGFG010000000
VERSION     DATGFG010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398169"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VKQ52_19080"
     CDS             <1..1447
                     /locus_tag="VKQ52_19080"
                     /inference="COORDINATES: protein motif:HMM:NF019130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89370.1"
                     /translation="LRVYLSVRFSSPIDIGGLKTADPLDPAVRDWWRQKADDIYRRIP
                     DFGGFLVKANSEGQPGPQNYGRTHADGANMLADALAPHQGIVIWRAFVYSNETPEDRA
                     RQAYTEFQPLDGRFHGNVLVQVKNGPIDFQPREPFHPLFGAMPNTPLMLEVQLTQEYL
                     GASTHLVYLAPLFEECLDADTHQPYPSQRPDPSGAAPLPTTVPGSAGTPVSATISGMA
                     GVANIGNDRNWCGHIFAQANWYAFGRLAWETGKATPCSARIATEWIRQTFSNKPSVVD
                     SIKQLMMASREITVDYMTPLGLHHIMAYEHHYGPGPWLANASRPDWNPVYYHKADSAG
                     IGFDRTATGSNALGQYAPGARLQWQNLDDCADQWLLWWHHAAWNHRMHTGRTLWAELC
                     HKYYSGVDSVRWMQHCWAALRQSIDPERWQQVNMRLTIQEQNAVEWRNACLLYFQTFS
                     HQPLPAGAEQPDHTLDYYEQHKLYYVPGTNL"
     gene            1425..2198
                     /locus_tag="VKQ52_19085"
     CDS             1425..2198
                     /locus_tag="VKQ52_19085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ89371.1"
                     /translation="MSQEQTFSSTTAPSRIAVITGGGSGIGLAIAEKFVEANILTIIV
                     GRDTGKLAAAKGRLGPLCHAITQDLNDLGAIPGLIRRITTEFGPVDILVNNAGINMKK
                     EFADVTDEDFESILHTNVRSVFAMCREVVRTMLPRGKGNIINISSMASQYGIPKVIAY
                     TASKSAIEGMTKAMAVELSPMGILVNCIAPGFIATDMSSKALNGDAERKQKVFSRTPL
                     GRLGEPADIGDAALFLASDQAKYITGVVLPIDGGNSVGF"
BASE COUNT          513 a          825 c          572 g          396 t
ORIGIN      
        1 cctccgggtc tatctctccg tccgcttcag cagcccgatc gatatcggcg gcctaaaaac
       61 ggccgacccg ctcgaccccg ccgtccgtga ctggtggcgt caaaaagccg atgacatcta
      121 tcgccgcatc cccgacttcg gcggcttcct cgtcaaggcc aactccgaag gccagcccgg
      181 tccgcagaac tacggccgca cccacgccga cggcgccaat atgctcgccg acgccctcgc
      241 cccacaccag gggatcgtca tctggcgggc cttcgtatat agtaacgaga cccccgaaga
      301 ccgggcccgg caggcctata ccgaattcca acccctcgac ggccgcttcc atggcaacgt
      361 actggtacag gtaaagaacg gccccatcga cttccagccc cgcgaaccct tccaccccct
      421 gttcggggcc atgcccaata cccccctgat gctggaagtc cagctcaccc aggaatacct
      481 cggcgcatcc acacacctcg tctacctcgc ccccctcttc gaagaatgcc tcgacgcgga
      541 cacccatcag ccctacccat cacaacgccc cgatccatcc ggcgccgccc cgctcccgac
      601 gaccgtccct ggctcagcgg gcacccccgt ctcggcaacc atcagcggca tggccggcgt
      661 agccaacatc ggcaacgacc gcaactggtg cggccatatc ttcgcccagg ccaactggta
      721 cgccttcggc cggctcgcct gggaaaccgg caaagccacc ccctgttcgg cccggatcgc
      781 caccgaatgg atccgtcaaa ccttcagcaa caaaccatcc gtcgtcgaca gcatcaaaca
      841 actgatgatg gcatcaagag agatcaccgt cgattatatg accccgttag gcctgcatca
      901 catcatggca tacgaacatc attatggacc tggtccctgg ctcgccaatg cttcccgccc
      961 ggactggaac cccgtatact accataaggc cgactccgcc ggcatcgggt tcgaccgcac
     1021 cgccacaggc agtaacgccc tcgggcaata tgcccccggc gcccggctcc aatggcagaa
     1081 cctggacgac tgcgccgacc aatggctgct ctggtggcac cacgccgcct ggaaccatcg
     1141 catgcacacc ggccgcacgc tctgggccga gctctgtcat aaatactaca gcggcgtgga
     1201 ctccgtccgc tggatgcaac attgctgggc tgctttacga caaagtatcg atccggaaag
     1261 atggcagcag gtgaacatgc ggctgacgat ccaggaacag aacgcagtgg agtggcgcaa
     1321 cgcctgcctc ctctacttcc agacattctc gcaccaaccc ctgccggccg gcgccgaaca
     1381 acccgaccat acccttgact attatgagca acataaactt tattatgtcc caggaacaaa
     1441 cctttagtag cacaacagca ccatcccgta tcgcggtcat caccggcggg ggctcaggca
     1501 tcggcctggc catcgcggaa aaattcgtag aggcaaacat cctcaccatc atcgtcggcc
     1561 gtgacaccgg gaaactggcg gcagccaaag gccgattggg tcccctttgc cacgccatca
     1621 cccaggacct caacgacctc ggcgccatcc ccggccttat ccggcgcata accaccgaat
     1681 tcggaccggt cgatatcctc gtgaacaacg ccgggatcaa catgaaaaag gagttcgccg
     1741 acgtgacgga cgaggatttc gaaagcatac tccacaccaa tgtccgctcc gtcttcgcca
     1801 tgtgccggga agtggtaagg accatgctgc cccgcggaaa aggcaatatc atcaacatca
     1861 gttccatggc ctcccaatac ggtatcccca aggtcatcgc gtatacggcc tccaaatccg
     1921 ccatcgaagg gatgacaaag gcaatggccg tagaactctc gccgatgggt atcctcgtca
     1981 actgcatcgc ccccggcttt atcgccaccg atatgtcttc aaaggcgctg aacggcgacg
     2041 cggaaagaaa acaaaaggtc ttctcccgta cccccctggg caggctgggc gagcccgccg
     2101 atatcggtga cgccgccctc ttcctggcct ccgatcaggc caaatatata acgggtgtcg
     2161 tgctgcccat cgacggcggc aactccgtcg gtttttaaaa ccttcgatat gaaaaaatgt
     2221 atcgtatgcc tgctgtccgc cattatggcg ctgacggcag ccgcgcagga ttttcccctt
     2281 gtctcgcaac aagagggggt cgcgca
//
LOCUS       DATGFG010001006         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_50950, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001006 DATGFG010000000
VERSION     DATGFG010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50950"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VKQ52_19090"
     CDS             complement(<1..182)
                     /locus_tag="VKQ52_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89372.1"
                     /translation="MEPTMQSNLFELQVDQTAMSYLKDAARWARFLAIAGFIFCGLFV
                     VVAIIFATVMANLFNT"
     gene            complement(345..1820)
                     /gene="proS"
                     /locus_tag="VKQ52_19095"
     CDS             complement(345..1820)
                     /gene="proS"
                     /locus_tag="VKQ52_19095"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HLZ89373.1"
                     /translation="MSKEITSRAQDYSQWYNDLVIKGGLADHSEVRGCMVIKPYGFAL
                     WENMRDQLDRMFKDTGHVNAYFPLFIPKSFLSREAAHVEGFAKECAVVTHYRLMNDPN
                     GGGVVVDPAAKLEEELIIRPTSETIIWNTYRDWIRSYRDLPLLINQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATADEAVAETRQMLDVYASFAEEYMALPVIKGIKTESERFA
                     GAVDTYCIEALMQDGKALQAGTSHFLGQNFAKAFEVKFSDRNNQLDYVWATSWGVSTR
                     LIGGLVMAHSDDDGLILPPRIAPLQVVIVPIYKGDERKQALDAKGRELAASLKAAGIR
                     VKYDDNDNNRPGFKFAEYEMKGVPVRIAMGLRDLDNNTVEVARRDTKEKSSVPMEGLA
                     AHIGRLLENIQQSMYDKAKAYRDEHITRADTMAEFERLLDGKGGFVLAHWDGTAATEE
                     AIKEQTKATIRCIPLNNPAEEGKCILTGKPSKQRVLFARAY"
     gene            1969..2778
                     /locus_tag="VKQ52_19100"
     CDS             1969..2778
                     /locus_tag="VKQ52_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89374.1"
                     /translation="MKRIYTLIAVATVVVFGLSSCSSSRNAQTTDDVYYSSGSQRTAG
                     SNYSSGGASQGDYYSTAPSDNYVRMKAEDPARWSYFDDYGAYDSYYAPAGVSMSYGYG
                     WGYPAYGMGYSPFGFGYGMGFGYGLGFCDPYMAWNSYFLWNSWYNPYFYNPYYGGGGF
                     VVGHSTPTAIYSNLRPFNSVAYRNGLSHNVGRTGTGSGRYYRPGMSTTSGYNSGLNGQ
                     GRQVNNSFYRPSNTNNNNGGYRPAFSQPTRSFSPSFGGGGGGGGFSRPGRH"
     gene            2863..>3432
                     /locus_tag="VKQ52_19105"
     CDS             2863..>3432
                     /locus_tag="VKQ52_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89375.1"
                     /translation="MHKHLFIIGIFLCSQVKAQLPEDALRASWTTPGGTAREQAIGGA
                     MGSLGGDITAAYVNPAGLGMYKTSEIVFSPGWRLLLDKGSYLGQNHTAPSMNKFAFGA
                     SGVVLGYEGVLPGVNNAFVISVNRSADFSSHVNYQGVNTYSSFSEQYVEEFASSGLDI
                     NGGIFSPSLSYGTRMALYTSLIDTATINGT"
BASE COUNT          699 a         1021 c          899 g          813 t
ORIGIN      
        1 atggtattga agagattggc catcaccgtc gcgaaaataa tggccaccac cacaaagagc
       61 ccgcaaaaaa taaagcccgc gatagccagg aacctggccc agcgggcagc gtctttgagg
      121 taggacatgg cggtctgatc aacctgcaat tcgaaaagat tgctttgcat cgtaggttcc
      181 ataacggggt gtagttttgc tacgccctcg tgccggccgg aaagatgtgc actcgggctt
      241 aagcggttat ttaattggcc ttcggccgat ggacctccgg tccattgcct aagataaaca
      301 aaatcctgat acagcgccgc agtccgtcaa accgataccc gggcttaata cgcccgggcg
      361 aacaataccc gctgtttgct gggtttaccc gtcaggatgc atttcccttc ctcggcgggg
      421 ttgttcagcg ggatacagcg gatggtagcc tttgtctgtt cctttatcgc ctcttcggta
      481 gcagccgtgc cgtcccagtg ggccaggaca aagccaccct tcccgtccag cagccgttcg
      541 aactccgcca tggtatccgc acgggtgata tgttcatcgc gataggcctt cgccttgtcg
      601 tacatgcttt gctggatatt ctccagcaac cggccgatat gcgccgccag cccctccatc
      661 gggactgagc ttttttcctt cgtatcccgg cgggccactt cgacggtgtt attgtcgagg
      721 tcacgaagtc ccatggctat ccgcaccggg acgcccttca tctcgtattc ggcaaatttg
      781 aaaccgggcc ggttgttgtc gttgtcatca tacttcacgc ggatgccggc agcctttagg
      841 gaggcggcga gttcccggcc ttttgcatca agggcctgct ttcgctcatc gcctttatag
      901 atgggcacga tcactacctg caggggagcg atcctcggtg gcaggatgag cccgtcatcg
      961 tcgctatgcg ccatgaccag gccgccgatc agcctcgtgc tgacgcccca gctcgtagcc
     1021 cagacataat ccaattggtt gttcctgtcc gagaatttca cttcaaaggc cttggcaaag
     1081 ttttgtccga ggaagtgcga ggttccggcc tgcaatgcct tgccgtcctg catcagggcc
     1141 tcgatacaat aggtgtccac ggcgccggcg aagcgctcac tctccgtctt tatcccctta
     1201 atgacgggca gggccatata ctcctcggca aaactggcat agacatccag catctggcgg
     1261 gtttcggcta cggcttcgtc ggccgtcgca tgggccgtgt gcccttcctg ccagaggaat
     1321 tccgccgtcc gcaggaaaag ccgggtgcgc atctcccagc gcaccacatt ggcccactgg
     1381 ttgatgagca ggggcagatc gcggtagctc cggatccagt cccggtaggt gttccagatg
     1441 atggtctcgc tggtgggccg gatgatgagc tcttcttcca gctttgccgc ggggtccacc
     1501 actaccccac ccccgttggg gtcgttcatg agccggtagt gcgtgaccac ggcgcattcc
     1561 ttggcaaaac cttccacatg cgcggcttcc ctgctgagaa agctcttggg aatgaacagg
     1621 ggaaaatagg cattgacgtg accggtatcc ttgaacatcc ggtccagctg gtcgcgcata
     1681 ttctcccaga gcgcaaagcc atagggcttg atgaccatac agccccggac ttcggaatgg
     1741 tcggcaagac cacccttgat caccagatcg ttataccatt gcgaataatc ctgggcccgg
     1801 cttgtaattt ctttactcat ttgctatcct tgtatataaa ttattaatca gcttttaaca
     1861 agaaaatgtt aagatttccg gttaaggcac attatttatt gtaactttac cttaacacga
     1921 agggttgcaa aaatagcaac ttcgacgtta aaataaattg atattgccat gaaacgcata
     1981 tataccctaa tagccgtagc cacggtcgtc gtgtttgggt tgagcagctg ctcttcctca
     2041 cgcaatgcgc agaccaccga cgacgtctat tattcttccg gctcgcaacg tactgcaggc
     2101 tccaattatt cttccggcgg agccagccag ggagactact acagcaccgc gccaagcgac
     2161 aactatgttc gcatgaaagc ggaggatcct gcccgctggt cctatttcga tgactacggc
     2221 gcttacgatt cttactatgc gccggcgggc gtctccatga gttatggtta tggctggggt
     2281 tatcccgctt atgggatggg ctattccccc tttggcttcg gctatggcat gggctttggc
     2341 tacggcctgg gtttctgcga tccctacatg gcctggaaca gttatttcct ctggaatagc
     2401 tggtacaatc cttatttcta caatccttac tatggcggtg gggggtttgt cgtaggccat
     2461 tcgactccga cggcgatcta ttcgaacctg cgtcccttca attcggttgc ctatcgcaac
     2521 ggcctttccc acaacgtggg caggaccggc accggctccg gacgctacta ccgccccgga
     2581 atgtctacca cttccggtta taattccggt ctcaatggtc agggcagaca ggtgaacaat
     2641 tccttctacc ggccttccaa caccaacaac aataacggcg gataccggcc ggccttcagc
     2701 cagcccaccc gcagtttctc gccctccttt ggcggcggcg gtggtggtgg cgggttcagc
     2761 aggcccggac gtcactaatt tacggatcac gccatccctt tcctttccct gatgctttct
     2821 gcctcaagag gcagtgtgcg aggctaacct aacaccaatc acatgcataa gcacctgttt
     2881 atcattggga tatttctatg ctcacaagtg aaggcgcagc tccccgaaga tgcccttcgc
     2941 gcctcctgga cgacacccgg cggaactgcc cgtgaacaag ccatcggcgg ggcgatgggc
     3001 tccctcggcg gcgatatcac cgccgcctat gtcaatccgg cgggactggg gatgtacaag
     3061 accagcgaga tcgtcttcag ccccggctgg cgcctgcttt tggacaaggg cagttatctc
     3121 ggtcagaacc ataccgctcc ttcgatgaac aaattcgcct tcggcgccag cggcgttgtc
     3181 ctcggctatg aaggcgtcct gccgggcgtc aacaacgcct ttgtcatctc cgtcaaccgc
     3241 agcgccgatt tcagcagcca tgtcaattac cagggggtca acacctatag ctccttttcc
     3301 gaacagtatg tggaggagtt cgcctcctcg ggcctcgata tcaacggcgg gatcttcagc
     3361 cccagcctct cttacggcac ccggatggcg ctgtatacat ccctgataga tacggctacg
     3421 atcaacggaa cg
//
LOCUS       DATGFG010001007         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_740829, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001007 DATGFG010000000
VERSION     DATGFG010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740829"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VKQ52_19110"
     CDS             <1..363
                     /locus_tag="VKQ52_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89376.1"
                     /translation="QMNLQNTVPDTLDPVEVGTNKPKEAESGFVDLVGQISLKTLEKA
                     DRKKKHGHHKHHSQHSSGRGGNPENSGGRPPGAEGGKDSGNRGGKDGRGGNQGNANNS
                     REPKKTGPPPPPDRSKKR"
     gene            563..1333
                     /locus_tag="VKQ52_19115"
     CDS             563..1333
                     /locus_tag="VKQ52_19115"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HLZ89377.1"
                     /translation="MALFESGNPTLSEKIFNRSVGGSQTESMTVKGSIQKFGFLMLLV
                     FAGAAYTWKLFYEFKVPTMMTLMWVGLIGGLVSAIIISFKPTLARYLAPAYGLLEGLA
                     LGALSAVINESVKGKYPNIVMQAVLLTFTVAFAMFLLYNFRIIRPTQKFKAIILSASL
                     GIGLFYVVWIVLSLFGVHFSFMSWNDASPLGIGINLFVIAIAALSLILDFERLELGEN
                     AGAPKYMEWYGAFGLLVTMVWLYIQILKLLSRLASSRN"
     gene            complement(1384..>2433)
                     /locus_tag="VKQ52_19120"
     CDS             complement(1384..>2433)
                     /locus_tag="VKQ52_19120"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ89378.1"
                     /translation="IRNLAVQRKNLGDLLNMNITFRDIAGGGAIKNVPISSLVHIDYT
                     STLGSVKRKNQKRVITLKSNVLNGFTPTAVNQQLARQIADFKKKPDGVTITQTGEGQQ
                     QAETGAFLLKALVIALATILGILVLQFNSVSKSVIILNEILFSVIGVILGFAITKMEV
                     SVIMTGLGIVGLAGIVVKNGILVIEFTDELRGRGLKTRAAAIEAGKTRIIPVLLTAMA
                     AILALIPLAVGFNINFVTLFSDLNPHIYFGGDNAVFWKPLSWTIIFGLAFAFFMTLFM
                     VPSMYLLAERLKRPMRRHWGGKWISFLGIPPFTIIFLLLVLITIFRHSYEVARRRRRL
                     QGRPGASETWIRSWF"
BASE COUNT          507 a          573 c          776 g          577 t
ORIGIN      
        1 caaatgaatc tccagaatac ggtgccggat acgctagacc cggtggaagt cggtacgaac
       61 aagcccaagg aggcggagag cggttttgtc gacctggttg ggcagatcag tctgaagacc
      121 ctggaaaagg ccgaccgcaa gaaaaagcat ggtcatcaca agcatcattc ccagcatagt
      181 tccggcaggg gtggcaaccc ggagaattcc ggcgggcgcc ctcctggcgc cgaaggcggc
      241 aaggatagtg gtaaccgggg tggcaaggat ggcaggggcg gcaaccaggg gaatgccaat
      301 aacagccggg agccgaagaa gaccgggcct cctcccccgc ctgacaggag caagaagcgc
      361 tgatccctct tttggattgt taaagttgta ctgacagttt agcaggctgg gggttccgtt
      421 gttatttttg tatacaattt gtacattgtt gcttacaatg cggaccacgg gtccgcaggc
      481 cgaaggccca tcattagccg tgcaccgggg gatgggttaa attcggatca aacccatccc
      541 cggcgcacaa aaactcaata ctatggcact ttttgaatcc ggtaatccta ccctgtcgga
      601 gaagatcttt aacagaagcg ttggcggttc gcagacggag tcgatgaccg tgaagggctc
      661 gatccagaaa ttcggttttc tgatgctgct tgtgttcgcg ggcgctgcgt atacctggaa
      721 attgttctat gaattcaagg ttcccacgat gatgaccctg atgtgggtgg ggcttatcgg
      781 cgggcttgtt tccgccatca tcatttcttt caagccgacg cttgcgcgct atcttgctcc
      841 cgcctacggg ttgttggagg ggcttgcttt gggtgcgttg tcggcggtga taaatgagtc
      901 ggtgaagggt aagtatccca atattgtgat gcaggcggtg ttgctgacct ttacggtggc
      961 cttcgccatg ttccttttgt ataatttccg gatcatccgg ccgacgcaaa aattcaaggc
     1021 tatcattctt agtgcgagcc tgggtatcgg gcttttctac gtggtgtgga tcgtgctcag
     1081 tctcttcggg gtacatttta gctttatgag ctggaatgac gccagtccgt tgggtatcgg
     1141 catcaatctt tttgtgatcg cgatcgcggc gctgagcctg atcctcgatt ttgagcggct
     1201 cgaactgggc gagaatgccg gcgctcccaa gtatatggaa tggtatggtg catttggttt
     1261 gctggtgacg atggtttggt tgtatatcca gatcctgaag ttgctgagcc gtttagcgag
     1321 cagcaggaat taaaagaatt cgaataaaaa aaggcccgtc ggagacgggc ctttttttat
     1381 tatttagaac caggacctga tccaggtttc gcttgctccg gggcggcctt gcaaccggcg
     1441 acggcggcgg gctacctcgt aagagtggcg gaagatggta atgagaacca ggagcaggaa
     1501 gatgatcgtg aacggcggga tgccgaggaa ggagatccat tttccgcccc agtgacggcg
     1561 catcggccgc tttagtcttt ccgccagcag gtacatgctt ggcaccatga acagggtcat
     1621 gaagaaggcg aaggccaggc cgaagatgat ggtccaggac aggggtttcc agaagactgc
     1681 gttgtccccg ccgaagtaga tatgcggatt caggtcggag aacagggtaa cgaaattgat
     1741 attgaagccg acggccaggg ggatcagcgc caggatggcg gccatggcgg tgagcagtac
     1801 ggggatgata cgggtcttcc ccgcttcgat ggctgctgcc cttgtcttga ggccgcggcc
     1861 gcgcagttcg tcggtgaatt cgatcacgag gatgccgttc ttgacgacga tacccgcgag
     1921 accgacgata cccaggccgg tcatgatgac ggatacctcc atttttgtaa tggcgaagcc
     1981 gaggatgacg ccgatgacgc tgaagagtat ctcgttcagg atgatgaccg atttgcttac
     2041 ggagttgaac tggagtacca ggatgcccag gatggtcgcg agggcgatga cgagggcttt
     2101 gagcaggaag gcgccggtct cggcctgttg ctggccttcc cctgtctggg tgatggtgac
     2161 gccatcgggt ttctttttga aatcggcgat ctgccgggcc agttgctggt tgacggcggt
     2221 gggggtaaag ccgttgagca cgttcgattt cagggtgatc acgcgtttct ggttcttgcg
     2281 ttttacgctg cccagcgtgc tggtgtagtc gatgtgcacc agggagctga tgggtacatt
     2341 tttgatggcc ccgccgccgg cgatatcccg gaaggtgatg ttcatgttga ggaggtcgcc
     2401 gaggttcttg cgctgaacgg ccaggttgcg gat
//
LOCUS       DATGFG010001008         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_56945, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001008 DATGFG010000000
VERSION     DATGFG010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56945"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="VKQ52_19125"
     CDS             <1..1617
                     /locus_tag="VKQ52_19125"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HLZ89379.1"
                     /translation="NFKLASLLVLVNGSLLFLAGICLSARRPAQKNAQSITIDYTKQT
                     HPQSHFWAFFGYDEPNYTYMRDGKKLLTELSALSPTPVYVRCHNLLTTGDGTAALKWG
                     STNAYTWDANGNPIYDWHIVDSIFDTYVQRGMKPLAEIGFMPEALSTHPEPYRHHWKP
                     GVRYDSIYTGWAWPPTDYTKWATLIEQWVRHCIQRYGEKEVATWLWEVWNEPNISYWK
                     GTEDEYLKLYDYSAKAVKKALPGARVGGPATTGPGGNNAARWLRDFLDHCADTKTPLD
                     FISFHAKGSPRIVDGHVRMNMEPQLKDVSNGFAIVKASSYSDLPIYITECDPEGCAAC
                     GMATNPENAYRNGTMYSSYTAASFARIATLADSCQVNLAAAMSWSFEFENQKWFDGFR
                     DLATNGVDKPVLNVFRMYGLMHGNRIPVDNAFVTADKHSLSVMVWNYTADDLPGPAET
                     RTLSITNLPAQKIKLTHYRIDDSHSNAYEVWKKMGSPQNPTPAQYRELEAAGQLQQLD
                     KPQTLPVKDGVLTIPVTLPRQAVSLIKLDF"
     gene            complement(1646..2062)
                     /locus_tag="VKQ52_19130"
     CDS             complement(1646..2062)
                     /locus_tag="VKQ52_19130"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ89380.1"
                     /translation="MKNNSGKIGIASERNGGGGWRDEEILTAENCPVRQVLDRIGDKW
                     STLTVLMLGKHGVMRFNELSQAIGDVSQKMLTVTLRSLEADGLVSRTMYAEIPPRVEY
                     ELTGMGRSLLPLITALKGWAEENIGEIKRNRKKFAR"
BASE COUNT          526 a          789 c          445 g          429 t
ORIGIN      
        1 aattttaagc tggcatcact gctggttttg gttaatggat ccctgctatt cttagcgggg
       61 atttgtttgt cggcccggcg tcctgcccaa aagaacgccc aatccatcac catcgactat
      121 acaaaacaaa cccatcccca atcgcatttc tgggccttct tcggctacga cgaacccaac
      181 tacacctata tgcgggacgg caaaaaattg ctcaccgaac tctccgccct cagccccaca
      241 cccgtctacg tccgctgcca caacctcctc accaccggcg acggcacagc cgccctgaaa
      301 tggggctcca cgaatgccta tacctgggac gccaacggca accccatcta tgactggcac
      361 atcgtcgaca gcatcttcga cacctatgta caacgcggca tgaaacccct cgccgagatc
      421 gggttcatgc ccgaagccct ctccacccat cccgaaccct atcgccatca ctggaaaccc
      481 ggcgtgcgct atgacagcat ctacaccggc tgggcatggc cgcctacgga ctatacaaaa
      541 tgggcaaccc tcatcgaaca atgggtgcgg cattgcatcc aacgctacgg cgaaaaagag
      601 gtggccacct ggctctggga agtctggaac gaaccgaata tcagttactg gaaaggcacc
      661 gaagacgaat acctgaagct ctatgactat tcggcaaaag ccgtcaaaaa agcccttccc
      721 ggcgcccgcg tcggcggccc cgccaccacc gggcccggcg gcaacaacgc cgcccgctgg
      781 cttcgcgact tcctcgacca ctgcgccgac acaaaaactc ccctcgactt catcagcttc
      841 cacgcaaaag gcagccccag gatcgtcgac ggtcacgtac gaatgaatat ggaaccccag
      901 ctaaaagacg tcagcaacgg cttcgccatc gtcaaagcct catcgtatag cgatctcccc
      961 atctatatca ccgaatgcga ccccgaaggt tgcgccgcct gcgggatggc taccaacccc
     1021 gagaacgcct atcgcaacgg aacaatgtat tccagctata ccgccgcctc cttcgcccgt
     1081 atcgccactc tcgccgatag ttgccaggtc aacctcgccg ccgccatgag ctggtccttc
     1141 gagttcgaga accaaaaatg gttcgatggc ttccgcgacc tcgcaaccaa tggtgtcgac
     1201 aaacccgttc tgaatgtctt ccgcatgtac ggtctcatgc acggcaaccg catccccgtc
     1261 gacaatgcct ttgtcaccgc cgataaacat tccctatccg tcatggtatg gaactacacc
     1321 gccgacgacc tcccgggccc cgccgaaaca agaaccctct cgatcaccaa cctccccgca
     1381 caaaaaataa agctcaccca ctatcgcatc gacgatagcc acagcaatgc ttatgaagta
     1441 tggaaaaaaa tgggctcccc gcaaaaccca acgcccgccc aataccgcga acttgaagca
     1501 gccggccaac tccaacaact cgataaaccc caaaccctcc cggtaaagga cggcgtcctg
     1561 accatccccg tcaccctccc ccggcaggca gtatcgctga taaaactgga cttttaagcc
     1621 gccgagtcgg cacctccggc ctggactaac gcgcaaactt cttcctgttc ctttttatct
     1681 ccccgatatt ctcctccgcc caacccttca acgccgtgat cagcggcagc aaactcctgc
     1741 ccattcccgt cagctcatat tccacccgcg gcgggatctc cgcatacatc gttcttgaca
     1801 ccaacccatc tgcctccagg ctccgcaacg tcaccgtcag catcttctgc gacacatccc
     1861 cgatcgcctg cgaaagctca ttgaatcgca tcaccccgtg cttccccagc atcaggacgg
     1921 tcagcgtcga ccacttgtcg ccgatgcgat ccaacacctg ccgcaccggg caattctccg
     1981 ccgtcaaaat ttcttcatct ctccaccccc caccaccatt ccgttcactc gcgatcccga
     2041 tttttcccga attatttttc attctattcc attgatgttc aataatagtt tccccggcgt
     2101 aactccgttc cccaggagtg ccttcttggc cggaacccgc gaagcgccta cctttacttt
     2161 ctaatagtaa ctaaatcact atacaaaag
//
LOCUS       DATGFG010001009        11565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1655477, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001009 DATGFG010000000
VERSION     DATGFG010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655477"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..810)
                     /locus_tag="VKQ52_19135"
     CDS             complement(265..810)
                     /locus_tag="VKQ52_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89381.1"
                     /translation="MKGSLIVRPVCKDTDRTLVRELFRQEFYGDLPQLYPDEGLWEIY
                     DSMETHDAFGAYLVFYHDRLLFLMEVHPPVQMDLAADYLSRPGTIGIYSFYASRIEPM
                     NLPALRACIDALLGHPTIERIVTRVSFVTPRDPRVDLLEKAGFRRLAKSTDKSAIYYC
                     TQDTFPLLGRNTNSRLHTVVE"
     gene            complement(1126..1911)
                     /locus_tag="VKQ52_19140"
     CDS             complement(1126..1911)
                     /locus_tag="VKQ52_19140"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HLZ89382.1"
                     /translation="MKKIIIAICLLTTLHISATPIPAAPKQEFYSIRIYQLKNTEQET
                     RVDSYLQTALLPALHRQGINDIGVFKPVGNDTAAIRRIYVLIPLKSLEQYTALSATLQ
                     KDSRYLADGKDYLDAPHDNPPYIRIESILLQAFPDMPHHAAPGSLHGTPAEKVYELRS
                     YEGPTEKYFDNKVQMFNAGGEIPLFDRLGFHAVFYASVLAGAHMPNLMYMTSFENMTS
                     REQHWKDFSADPVWKKLVAEPQYQHNVSHVDIVFLHPAPYSDL"
     gene            2055..2774
                     /locus_tag="VKQ52_19145"
     CDS             2055..2774
                     /locus_tag="VKQ52_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89383.1"
                     /translation="MRNITILLLLLCLVACLQGSGQQFLTGKIYKVNSTEFLISVSIH
                     NITQQRYDLSEEDGSYRIQAAPGDHISFSSVGYKADTITVSASLLTASCPIYLDVRAQ
                     TLQTVRVGQFSNYQLDSMDRRKEYAWVYEHENTPRMARDRQGADGVGVTLNIFRNTGS
                     AAKQRIHLEKRLEKEEQEYYVDSRYNKDYVSKITRLKGDSLSQFMRKYRPSYDYCRRA
                     ANVDILVYISDSYKQFMKGED"
     gene            2809..4527
                     /locus_tag="VKQ52_19150"
     CDS             2809..4527
                     /locus_tag="VKQ52_19150"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HLZ89384.1"
                     /translation="MKELRSRNWFGKTDKMGFIYRSWMKNQGFPEDLFDGRPVIGICN
                     TWSELTPCNAHLRDIAEVVKRGVYEAGGFPLEFPVMSLGETLLKPTAMLFRNLASMDA
                     EESIRGNPIDGVVLLTGCDKTTPSTLMGAASVDLPTLVVPGGPMLNGKFRGTDIGSGT
                     HVWKFDEERKKGQLSDADYLYAESCMSRSAGHCMTMGTASTMACMVESLGLTLPGAAA
                     IPAVDSRKKVLAHLSGRRIVEMVKEDLRLSRILTRRAFENAIRVNAAVGGSTNFIIHL
                     TAIAGRIGVPLELADFDRLGSGVPLLLNLMPSGKFLMEDFYYAGGLPVILNELRDLLD
                     LDAPTVNGRTHRENVAGREVCYNREVIAAYADPLIDHAGIAVLKGNLAVNGAVIKPSA
                     ATPALMQHRGRAVVFEDIEDYHRRIDDPGLDIDASCVMVLKNVGPVGYPGMPEVGNMA
                     LPKKLLAEGVTDMVRISDGRMSGTAYGTVVLHVSPEAAVGGVLALVKNGDWIELDVEG
                     RRLHLDVPEAELEKRRKAWAPPAVPGTGRGYVQLYRNHVQQAHEGADLDFLRGGSGAE
                     VSRDSH"
     gene            4596..5918
                     /locus_tag="VKQ52_19155"
     CDS             4596..5918
                     /locus_tag="VKQ52_19155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HLZ89385.1"
                     /translation="MHLLLVLLGILLLVVLISWVKLNAFLSFLVVAILTGLFLGIPFD
                     AITKSVYKGIGDTLGQLAIVIVLGAMLGKIVAESGAAQRIATTMMNAFGEKYIQWALM
                     ITGFIIGIPLFYNVGFVLMVPLIFSVVYKYKLPVIYIGLPMLASLSVTHGFLPPHPAP
                     AALVGLFHADMGKTLLYGMLLAIPAIILAGLLYSRTLKHLVARPLEGLVAAEKPEDQL
                     PGIGTSLFCSLLPVGLLMLTTVLSQRMSLSGQGKAVLSFFNDASVILLLAIGAATWLL
                     GIRRGMNIPKIMVIYGEAVKDIAMILLIVAGAGALKEVFTDSGVSREIATVLQGWPVH
                     PLVLGWLIAAIIRVCLGSATVAGLTTAGIILPLMNRLHVDPNLMVLSIGAGSLMFSHV
                     NDGGFWMFKEYFNLSIKDTIRSWSVMETIVSVVGLAGVLLINYVMVFI"
     gene            5972..6475
                     /locus_tag="VKQ52_19160"
     CDS             5972..6475
                     /locus_tag="VKQ52_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89386.1"
                     /translation="MEKTITKDILKSELYAGQRPAVELLYTRYSGMLFSYILQFVPDR
                     TEAGTLLVDIFSRLTPQLQEAFDSNLSIYCWLQVESRKILLEYIQSKGKTDAGEPVAP
                     SIGGNTRAYYFSLLEDAPPEHQWVFRELFLYGREKEELALHSGKDLAYISRVLRECFG
                     VIRKNLG"
     gene            6472..6963
                     /locus_tag="VKQ52_19165"
     CDS             6472..6963
                     /locus_tag="VKQ52_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89387.1"
                     /translation="MNIELYMQSGIIESYALGLATPEEAGEFEQLLPHYPELKDALSD
                     FEYHLELFSIDHEIPPPPDVREKIEARLRGVPVLPHAGGGQYRQQHGQLRSEYVQAKE
                     TSQHIRVHKVWRPLFIAVFILSKIFLVLAIYFFLEYRHARSQVQRLSEPAGRAQGAGS
                     EGK"
     gene            7181..8038
                     /gene="hisG"
                     /locus_tag="VKQ52_19170"
     CDS             7181..8038
                     /gene="hisG"
                     /locus_tag="VKQ52_19170"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HLZ89388.1"
                     /translation="MTRLKIAIQKSGRLYDDSVKLLKDCGIDLKNGVNKLKAECDNFP
                     MEIYFLRDDDIPQYVEDAVADIGIVGENVFYEKNKRAEIVEQLGFGKCRLSVAVGRSE
                     QYDGVSFLQGKKIATTYPELLRQFLDRNGVDAEIHEISGSVEIAPGIGLADAIVDLVS
                     SGSTLFMNGLKEVETVLKSQAVLIRNERLGVTQQQLLEKFLFRIRAVKKARNNKYILL
                     NAPNDKLKEIIRLLPGMKSPTVLPLAEAGWSSVHSVLSENDFWDIIEQLKDAGAQGIL
                     VVPIEKMVM"
     gene            8040..9338
                     /gene="hisD"
                     /locus_tag="VKQ52_19175"
     CDS             8040..9338
                     /gene="hisD"
                     /locus_tag="VKQ52_19175"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HLZ89389.1"
                     /translation="MKVIDIREKSVEAARELLKRPVIDVARLEETVGAILAEVRAGGD
                     AAVRKFSLQFDKVAPERPELSAEEITAGAAGLDEELKAAIRMAKENIFSFHQRQERQE
                     QVIETMPGVRCWRRGVAIEKVGLYIPGGTAPLFSTLLMLAIPARIAGCREIILCSPPD
                     AQGRLHPALLYAAGIVGVTRIFRVGGVQAIGAMAYGTESMPAVYKLFGPGNQYVTCAK
                     QLVQREGIAIDMPAGPSEVAVYADETADAGFVAADLLSQAEHGVDSQVVLVSPSSAVV
                     ERVLAAVDRQVALLPRAEIARKALVNSRAFIVKDAGQAMELLNDYAPEHLILATDDAE
                     RMAEGVINAGSVFLGHYSPESVGDYASGTNHVLPTNGAARAFSGVSLDSFVKKITFQQ
                     LSREGLGAIGGTVWQMARAEGLDAHARAVSIRIAGEAADK"
     gene            9254..10393
                     /gene="hisC"
                     /locus_tag="VKQ52_19180"
     CDS             9254..10393
                     /gene="hisC"
                     /locus_tag="VKQ52_19180"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HLZ89390.1"
                     /translation="MADGEGRGAGCACPGGEYTNSRRGGRQIAIIFNLDTLLRDNIRR
                     LVPYSSARDEFKGEASVFIDANENSLGSPLTKWYNRYPDPHQHAVKVAISKIKGIPPQ
                     HIFLGNGSDECIDLLFRAFCVPGKDNVIINPPTYGMYEVSAHINDIEVRRAVLLDDFQ
                     LDLVHLENLVDEHTKLIWICSPNNPTANSIDRQDIEVVLNNFSGLVVIDEAYINFSRH
                     KSFIQELGDYPNLVVLQTLSKAWGLAGLRLGMAFASEAIIDVYNKVKPPYNISQATQE
                     LALKALEEVGQVNDMIRDLVTMREQLSKDLAELSVVQRVYPSDANFLLVKVENARSAY
                     EHLLRAGIVVRDRSKVELCEGCLRITVGTEQENAELLKSLKTWQV"
     gene            10487..>11565
                     /locus_tag="VKQ52_19185"
     CDS             10487..>11565
                     /locus_tag="VKQ52_19185"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HLZ89391.1"
                     /translation="MMRKFLHCLTILSLLWPRDAASQLKLPTGFTAITVAEGLGRARH
                     LAVTPNGVVFVKLERLKDGKGIYRLEDSNGDGKADKITAFGNYGGTGMAIRDGYLYAS
                     SDEEVFRYRLDAANNVVAPDQPEKIVTGLLSRHEHEAKAITLDNSGNLYVNIGAYSNA
                     CQLKDRTKGSPGQMPCPILDSAGGIWQFKADQPNQTYGTGVRYATGLRNVVGLDWNTA
                     TNTLFVMMHGRDQLSSLFPQYWTDKQSAELPAECMYELHKGSNCGWPYIFYDQIQHKQ
                     ILGPEYGGGGKKGPEKEYQDPVVAFPGHLAPDGLLFYTGTQFPEKYRNGAFVAFHGSW
                     NRAPEPQAGFFVVFVPFSDGKPSGN"
BASE COUNT         2450 a         2681 c         3774 g         2660 t
ORIGIN      
        1 gagggtaccc ctgataatgg cggctatggc cgggttatcc gcgggttcga tggtgcggat
       61 acgggtcctg atgagttcca tggcgcgaag ctacggcttt agctcaacag tgcgatgact
      121 ccatagagga cggctatgga gacgatgagg atgcctataa aaggcctgtc tgcgttcttc
      181 atgacgatcg aatttttcac gggtaaaatt gggtgattat tggtctaacg cagcagatag
      241 ggcaaatagt atgccctgtg gaaattattc gacgacggtg tggaggcggc tgttggtatt
      301 ccgtccgagg agggggaagg tatcctgggt gcagtaatag atcgcggatt tatcggtgct
      361 ttttgccagg cgccggaagc ctgctttttc cagcaggtcg accctgggat cccgcggggt
      421 tacgaagctg acgcgggtga cgatacgttc gatggttgga tggccgagga gggcatcgat
      481 acaggcccga agggccggga ggttcatcgg ttctatgcgg gaggcataga agctatagat
      541 gccgatggtg ccgggccggg aaagatagtc ggcggcgagg tccatctgga cgggtggatg
      601 gacttccatg aggaagagaa gacggtcgtg gtagaaaacg aggtaggcgc caaaagcgtc
      661 gtgggtttcc atactgtcat agatctccca tagaccttcg tcggggtaga gctggggaag
      721 atcgccatag aactcctggc ggaacagttc ccggacaagg gttcggtccg tatccttaca
      781 gacaggccga acaataaggg accccttcat gggcgatgag gttattgcta acatggcgtc
      841 ggaatggttt gaacagcagc catttgtttt gtttcgaaaa tggcttgaca tactaccgat
      901 cgggccttac aattcttacg atgtcttaac caggagtcgg agtcttaacc agttggtccg
      961 tgtgaagtct taaccagaag agtgtggagt aataggctgc gaagatgggt ataaatttga
     1021 aataacggaa atataacagg agctaaaacg cgcttctttt taattatttg taagaaacgg
     1081 taagggttaa cccgtaaatg gatgtcatgg atgatgtcaa ggccatcaga ggtcggagta
     1141 aggcgccggg tgcaggaaga cgatatcgac gtgcgaaacg ttgtgctggt attggggttc
     1201 ggccaccagc ttcttccaga cggggtcggc gctgaagtcc ttccagtgct gttcccggga
     1261 ggtcatattt tcgaaggagg tcatgtacat gaggttgggc atgtgggcgc cggccaggac
     1321 gctggcgtaa aaaacggcgt ggaagcccag gcggtcgaag agggggatct cgccgccggc
     1381 gttgaacatc tgtactttgt tatcgaaata tttttcggtg gggccttcgt agctgcgcag
     1441 ctcgtacact ttttcggcgg gtgtgccgtg cagcgagccg ggtgcggcgt ggtgcggcat
     1501 atcggggaag gcctggagca ggatggattc gatgcggata tagggcggat tgtcatgggg
     1561 ggcgtcgagg taatccttgc cgtcggcgag atagcggcta tccttttgca gtgtggcgga
     1621 gagtgcggtg tattgttcga ggctttttag cgggatcagc acatagatgc ggcggatggc
     1681 ggccgtatca tttcccacgg gtttgaatac cccgatgtcg ttgatgccct ggcggtggag
     1741 ggccgggagg agggctgtct gcaggtagct gtccactctt gtctcctgtt ccgtgttctt
     1801 tagctggtag atgcggatgg agtagaattc ctgtttgggg gcagcgggga tgggggtagc
     1861 ggaaatatgg agagtggtca ataagcagat ggcaattata atctttttca ttatgcaaat
     1921 gttgaatatg ttaggcgcca gccaattagt cctgataaat gtagaatagt attgccgttt
     1981 taacaaaaac ttggaggagg ccggcccgcc ggataccgga aataattcta tcttggcggg
     2041 ttcctgacta atctatgcga aatattacta ttctgttgct attgttgtgt ttggtggcct
     2101 gtctgcaggg aagcgggcag cagttcctta cgggaaagat ctataaagtc aacagtaccg
     2161 aattcctgat ctctgtaagt atccacaaca tcacgcaaca acggtatgac ctctccgaag
     2221 aggacggcag ttaccggatc caggccgcgc cgggggatca tatttctttt tcgtccgtag
     2281 ggtataaggc ggatacgatc accgtgagcg cctctctgct gacggcgtcc tgtcccatct
     2341 acctggatgt gcgggcgcaa accctgcaga ccgtgcgggt agggcagttc agcaattacc
     2401 agttggattc gatggaccgc cggaaggaat atgcctgggt ctatgagcac gagaacacgc
     2461 cgcggatggc gagggaccgc cagggcgccg atggggttgg ggtgacgctg aacatctttc
     2521 gcaatacggg ctccgccgcc aaacagcgga tccacctgga gaagcgcctc gagaaggagg
     2581 agcaggagta ttatgtcgat tcccgctaca acaaggatta tgtctccaag atcacccggt
     2641 tgaagggtga ttcgctttcg cagttcatgc gcaaataccg tccttcctac gactattgcc
     2701 gtcgcgcggc gaatgtggat attctggtat atatcagcga tagctataaa cagttcatga
     2761 aaggggaaga ttagttcatg aaggtataag attaggtata tttatcccat gaaagaactt
     2821 agaagccgga attggtttgg gaaaaccgat aagatgggtt tcatctaccg aagctggatg
     2881 aagaaccagg ggtttcccga agaccttttc gatggccggc cggtgatcgg catttgcaat
     2941 acctggtctg agctgactcc ctgcaacgca catttacggg acatcgccga agtcgtcaag
     3001 cgtggtgttt atgaggccgg cgggttcccg ctggagttcc ctgttatgtc gctcggggag
     3061 accctgctca agcccacggc gatgttgttc cggaacctgg cgagcatgga tgccgaggaa
     3121 tccatccggg gcaaccccat cgacggggtg gtattgctga cgggctgtga caagaccacg
     3181 ccatccacgc tgatgggggc ggcgagtgtg gacctgccta cgcttgtcgt gccggggggg
     3241 ccgatgctga acggaaaatt ccggggaacg gacatcggtt cgggtacgca tgtctggaag
     3301 ttcgatgagg agcggaagaa ggggcagtta tccgatgccg attacctgta tgcggagagt
     3361 tgtatgtcgc gaagtgcggg acattgtatg acgatgggca ccgcctctac gatggcctgt
     3421 atggtggagt cgctggggct gaccctgccg ggcgccgcgg cgatacctgc cgtggacagc
     3481 cggaagaagg tgctggcgca tctttcgggc cggcggatcg tcgagatggt gaaggaggac
     3541 ctgcggctga gcaggatatt gacaagacgg gccttcgaga atgccatcag ggtaaatgcc
     3601 gccgtcggcg gaagcaccaa ttttatcatc catcttacgg cgatagcggg gcgcatcggg
     3661 gtaccgctgg aactggcgga tttcgaccgt cttggcagtg gtgtgccgtt actgctcaat
     3721 ctcatgccat cgggaaaatt cctgatggag gacttttatt atgccggtgg attgccggtg
     3781 atcctgaacg agctgcggga cctgctcgac ctggatgcgc ctacggtgaa cggccgtacg
     3841 caccgggaaa atgtggcggg gcgggaggtc tgttataaca gggaggtcat tgccgcctat
     3901 gccgaccccc tgatcgatca tgcgggtatc gcggtattga agggtaacct ggccgtcaac
     3961 ggcgcggtga tcaagccttc cgctgctacg cccgcgctga tgcagcatcg tggccgggcg
     4021 gtcgtgttcg aggatatcga agactatcac cggaggatcg acgacccggg gctggatatc
     4081 gatgcttcct gtgtgatggt attgaagaat gtgggccctg tcggttaccc ggggatgccg
     4141 gaagtgggca atatggcgtt gccgaagaaa ctgctggcgg agggcgtgac ggatatggtg
     4201 cggatctcgg atggccggat gagcggcacg gcctatggta cggtggtgtt gcatgtctcg
     4261 ccggaggctg ctgtcggcgg ggtgctggcg cttgtaaaga acggggattg gatcgagctc
     4321 gatgtggagg ggcggcggtt gcacctggat gtaccggagg cggaactgga gaaaagaagg
     4381 aaggcatggg cgccgcctgc agtaccgggg acggggaggg gatatgttca actctatcgc
     4441 aaccatgtac agcaggctca tgaaggagcc gacctggatt ttctacgggg tggttccggc
     4501 gcggaggtga gccgggattc gcattaaccg ggattcgctt tattcggatt cgccataccc
     4561 ggattcgcta tacttaatac atcgatcaat cgcttatgca cttactgctt gttcttctgg
     4621 gcatcctgtt actggttgtc cttatttcct gggtaaagtt gaatgctttt ctgagttttc
     4681 ttgtcgtcgc tattcttacc ggcttgtttt tgggtattcc tttcgatgcg atcaccaaat
     4741 cggtgtataa gggtatcggg gatacgcttg gtcaattggc gatcgtgatc gtccttggcg
     4801 cgatgctggg gaagatagtg gcggagagcg gcgccgcgca gcgtatcgcc accacgatga
     4861 tgaatgcctt tggcgagaaa tatatccaat gggcgctgat gatcacgggt tttatcatcg
     4921 gtatcccgct tttctacaat gtgggattcg tgctgatggt gccgctgatc ttttcggttg
     4981 tgtacaagta caagctaccg gttatttata tcgggttgcc gatgctggca tccctgtcgg
     5041 tgacgcatgg tttcctgccg ccccatcccg cgccggcggc gcttgtgggg ctgttccatg
     5101 ccgatatggg caagaccctg ttgtatggga tgctgctcgc catcccggcg atcatcctgg
     5161 cggggctgct ttattcgcgt acgctgaagc accttgttgc ccggccgctg gaggggttgg
     5221 tggccgcgga gaagcccgag gatcaattac cgggcatcgg caccagcctt ttttgcagtc
     5281 tgctgccggt ggggctgctg atgctgacca cggtcctgtc ccagcggatg agcctgtccg
     5341 ggcagggcaa ggccgtgctg agttttttta acgatgcgtc ggtgatcctt ttgctggcta
     5401 tcggcgcggc cacctggctg ctggggatcc gccgggggat gaacatcccg aagatcatgg
     5461 tgatctatgg ggaggcggtg aaggacatcg cgatgatcct gctcatcgtg gcgggggcgg
     5521 gggccctgaa ggaggtgttc acggatagtg gcgtcagcag ggagatcgcg acggttctgc
     5581 agggctggcc ggtccatccg ctggtgctgg ggtggctgat cgcggcgatc atccgggttt
     5641 gcctgggttc ggcgacggtg gcggggctga cgacggcggg gatcatcctc ccgctgatga
     5701 accggttgca tgtcgacccc aacctgatgg tcctgtccat cggggcgggc agcctgatgt
     5761 tctcgcatgt caatgacggt ggcttctgga tgttcaagga gtacttcaat ctgtcgataa
     5821 aggacacgat ccggagctgg tcggtgatgg aaaccatcgt ttcggtggtt gggcttgcag
     5881 gtgttttact gataaattac gtaatggttt tcatttagta ttagctaatt ttagcacgct
     5941 ttcaccctgt ggaggtcaaa acctgtctat tttggagaaa acgattacga aggatatact
     6001 aaaaagcgag ttgtatgccg gtcagcggcc tgccgtggag cttttgtaca cacggtatag
     6061 cgggatgctg tttagctata ttctacaatt cgtacccgat agaacggaag ccgggaccct
     6121 tttggtggat attttctccc gccttacacc tcaactgcag gaagccttcg attccaatct
     6181 cagcatttac tgctggctgc aggtcgagtc aagaaagata ctcctggagt atattcaaag
     6241 caagggtaaa acggatgcag gcgaaccggt ggccccttct atcgggggca ataccagggc
     6301 ttattatttt tccctgttgg aagatgcgcc tcccgagcat caatgggtgt tccgggagct
     6361 gtttctttac ggacgggaaa aggaggagct ggcgcttcac tctggtaagg atcttgcata
     6421 tatcagcagg gttctgcgcg aatgtttcgg cgtcatcaga aaaaatttgg ggtgaatata
     6481 gagctgtata tgcaaagcgg cattatcgaa agttatgccc tgggtttggc cactccggag
     6541 gaggcgggcg aattcgagca gttgttgccg cattatcccg agttgaagga tgcgttgtcg
     6601 gatttcgaat atcatctcga gttgttctcc atcgatcatg agatcccgcc gccgccggat
     6661 gtgcgggaga agatagaggc gcgtttgcgg ggggtaccgg tgcttccgca tgcaggcggc
     6721 ggacagtatc gtcagcagca tggtcagctg cggagcgagt atgtccaggc gaaggagacg
     6781 tcgcagcata tccgggtgca taaggtctgg cggccgttgt ttatcgccgt gttcatcctg
     6841 tctaaaatat tccttgtcct tgcgatctat tttttcctgg agtaccggca tgcgcggagc
     6901 caggtccagc ggttgtcgga gccggcggga agggcgcagg gggcgggttc cgaagggaaa
     6961 taaaattaaa ttttggccat tgggaattcg ccattaattt tgggccgcaa aagggccggg
     7021 tttggccagg ccggagaaaa tatgatcaac aataaacaac atcatcacca tcattctttc
     7081 ttaccttagg taggccggtg atgttttgtc taaaacatat aattacaaag ggcttaccgc
     7141 aaggtaggcc ctcattattt ttaagaattt accatttact atgaccagat taaagatcgc
     7201 tattcagaag tcaggccggt tgtatgacga tagcgtaaaa ctgttaaaag actgcggcat
     7261 cgacctgaag aacggggtca acaagctaaa ggcggaatgc gacaattttc cgatggagat
     7321 ctattttctg cgtgatgacg atattccgca gtatgtggag gatgcggtgg ccgatatcgg
     7381 aatcgtgggg gagaatgttt tttatgagaa gaacaagagg gccgagatcg tggagcaact
     7441 ggggtttggg aagtgccggt tgtctgtggc tgtcggccgg agcgagcaat atgatggggt
     7501 aagctttctt caggggaaga agatcgcgac gacttacccg gagttgttgc ggcagttcct
     7561 ggataggaat ggggtcgatg ccgagatcca tgagatcagc ggttcggtgg agatcgcgcc
     7621 gggtatcggg ctggcggatg ccattgtcga cctggtcagc agcggcagca cgttgttcat
     7681 gaatggcctg aaggaggtgg agacggtgtt gaagagccag gcggtgctga tccggaacga
     7741 acggcttggt gtcacccagc agcagttgct ggagaaattc ctgttccgga tccgggcggt
     7801 gaagaaggcg cggaacaaca agtatatcct gttgaatgca ccaaacgaca agctgaagga
     7861 gatcatccgg ctgttgccgg ggatgaagag tccgacggtg ctgccgcttg cggaagccgg
     7921 ctggagcagt gtgcacagtg tgcttagcga aaatgatttc tgggatatca tcgaacaact
     7981 gaaggatgcg ggtgcacagg gtatccttgt cgtgccgatc gagaaaatgg tcatgtaata
     8041 tgaaagttat agacatacgg gagaagagcg ttgaggcggc gagggagctg ctgaagcggc
     8101 cggtgatcga tgttgcgagg ctcgaagaga ctgtcggggc gatattggcc gaggtgcggg
     8161 cggggggtga tgccgccgtg cggaagttct cgctgcaatt cgacaaggtg gccccggagc
     8221 ggcccgagct tagtgcggag gagatcaccg cgggggcggc cggcctggat gaagagttga
     8281 aggcggcgat ccggatggcg aaggagaata tcttttcctt tcatcagcgg caggagcggc
     8341 aggagcaggt gatcgagacg atgcccggtg tccggtgctg gcggcggggg gtggcgatcg
     8401 agaaggtggg gttgtatatc cccggcggta cggcgccctt gttctcgacc ctgttgatgc
     8461 tggccattcc cgcgcggatc gcgggttgcc gcgagatcat cctctgttct ccgcccgatg
     8521 cccagggcag gctgcacccg gctcttttgt atgccgccgg gatcgtcggg gtgacgcgga
     8581 tcttccgggt cggcggggtg caggccatcg gtgcgatggc gtatggaacg gagtcgatgc
     8641 ctgccgtcta taaactattc ggacccggca accagtatgt cacctgtgcc aagcaactcg
     8701 tacagcgcga gggtatcgcg atcgacatgc cggccggacc cagtgaagtg gcggtatatg
     8761 cggatgaaac ggcggacgcg ggattcgtag cggctgatct tttgtcccag gcggagcatg
     8821 gcgtggacag ccaggtcgtg ctggtgagcc cttcttccgc ggtggtggaa cgggtgcttg
     8881 ccgcggtgga ccggcaggtg gccttgctgc cgagggcgga gatcgcaagg aaggcattag
     8941 tgaacagccg ggcctttatc gtgaaggatg ccgggcaggc gatggagctg ttgaatgact
     9001 atgcgccgga gcatcttatc cttgccaccg acgatgcgga gcggatggcg gagggggtga
     9061 tcaatgcggg atcggtcttt ctcgggcatt attcaccgga gagcgtgggg gattatgcca
     9121 gcggcaccaa tcacgtattg cccaccaatg gcgccgcgcg ggcattcagc ggtgtgagtc
     9181 tcgacagttt tgtaaagaag atcacttttc agcaattgag ccgggagggg ctgggcgcca
     9241 tcgggggcac ggtgtggcag atggcgaggg ccgaggggct ggatgcgcat gcccgggcgg
     9301 tgagtatacg aatagccggc gaggcggcag acaaatagct attatattca acctagatac
     9361 acttttacgc gacaacatcc gtcggttggt gccgtattct tcggcccggg atgaattcaa
     9421 gggagaggcg agtgtcttta tcgacgccaa tgagaacagc ctcggcagtc cgcttacgaa
     9481 atggtataac cgctaccccg atccgcatca gcatgccgtg aaggtggcga tcagcaagat
     9541 caagggtatc ccgccgcagc atatctttct cggcaatggg agcgacgaat gtatcgacct
     9601 gcttttccgg gcgttttgtg tgccggggaa ggacaatgtg atcatcaacc cgcccaccta
     9661 tgggatgtac gaggtgagcg cacacatcaa tgatatcgaa gtccggcgag ccgttttgct
     9721 ggatgatttt cagctggacc tggtgcacct cgaaaacctg gtggacgagc ataccaagct
     9781 gatctggatc tgcagcccca acaatcctac ggccaattcc atcgaccggc aggatatcga
     9841 ggtcgtgctg aataactttt ccggcctggt tgtcatcgac gaggcctata tcaatttctc
     9901 gcggcacaag tcttttatcc aggagcttgg cgactatccc aatcttgtcg ttctgcagac
     9961 gttgagcaag gcctgggggc tggccgggct gcggctcggt atggcctttg catccgaagc
    10021 cataatcgat gtctacaaca aggtgaagcc gccctataat atcagccagg cgacgcagga
    10081 gctggcgcta aaggcattgg aggaagtggg acaggtgaac gacatgatcc gcgacctcgt
    10141 tacgatgcgg gagcagctga gcaaggacct ggcggagctg tccgttgtgc agcgggtgta
    10201 tcccagcgat gccaatttcc tgctggtgaa agtggagaat gcacggagtg cttacgagca
    10261 tctgttgcgt gcggggatcg tcgttcgcga ccgcagcaag gtggagcttt gcgagggttg
    10321 tttgcggatc acggtaggga ccgaacagga gaatgccgaa ttgctaaaat cgctgaagac
    10381 ctggcaagtc taagataaaa attagtgatg ccggggcaga tttttcgact aaattacggc
    10441 ttcttaacaa gcatattcta tgcaaccaat caaccaatcg aaaacgatga tgcgtaaatt
    10501 tttgcattgc ctgactattt tatccctgct ctggcccagg gatgcggcga gccagttgaa
    10561 attgccgacg ggtttcacgg ccatcacggt ggcggagggc ctgggcaggg cccgtcatct
    10621 ggcggtgacg cccaatggag tagtttttgt caaactggag cggctgaagg atgggaaggg
    10681 gatctaccgg ctggaggatt cgaacgggga tggcaaggcc gacaagatca cggcgtttgg
    10741 caattatggg ggtacgggca tggctatccg tgacgggtat ctctatgctt cttccgatga
    10801 ggaggttttt cgttaccggt tggatgctgc taacaatgtc gtcgctccgg atcagccgga
    10861 gaagatcgtg acggggctgc ttagccggca tgagcatgag gcgaaggcga tcacgctgga
    10921 taatagcggt aacctatatg tcaatatcgg cgcttattcc aatgcctgcc agttaaagga
    10981 ccggaccaag gggtcacccg ggcagatgcc ctgcccgata ctcgattcgg caggggggat
    11041 ctggcagttc aaggcggatc agccgaacca gacctatggc accggggtcc gctatgcgac
    11101 gggattgcgg aatgtggtcg gcctggactg gaacacggcg accaataccc tctttgtgat
    11161 gatgcatggc cgcgaccagc tgagctcgct attcccccag tactggaccg ataagcagag
    11221 cgccgagctg ccggccgagt gtatgtatga attgcacaag ggctccaact gcggctggcc
    11281 ctatattttt tacgatcaga tccaacacaa gcagatactt ggaccggaat atggcggtgg
    11341 tggcaagaaa gggcctgaaa aggaatacca ggacccggtg gtggcttttc ccggtcatct
    11401 cgcacctgac ggcctgttgt tctacaccgg cacacagttt cccgagaagt acaggaacgg
    11461 ggcctttgtc gccttccatg ggtcctggaa ccgggcgccg gaaccgcagg cgggcttttt
    11521 cgttgtgttc gtacctttta gcgatggcaa gcccagtggt aactg
//
LOCUS       DATGFG010001010         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1261742, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001010 DATGFG010000000
VERSION     DATGFG010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261742"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /gene="asnB"
                     /locus_tag="VKQ52_19190"
     CDS             complement(<1..1776)
                     /gene="asnB"
                     /locus_tag="VKQ52_19190"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HLZ89392.1"
                     /translation="MCGIAGILSADAAKITPQRLKNMTAAIAHRGPDGEGIWLSRSGL
                     AGLGHRRLSIIDLSPAGDQPMHYLDRYTIIHNGELYNYLELRSVLQSKGYAFTSRTDT
                     EVIVAAFDHYGVDCLQYFDGMFAFAIWDETKQQLFLARDRFGEKPLFFYRDTDQFLFA
                     SEAKALWAAGIKKEPNAKMLFNFLTIGYTQNPGNSFETFFEGISKLPARSFLIYQPAT
                     GHLETAVYWDIEAGQTAPIKEETAVDTFCQLLHASVHRRLRSDVRVGTSLSGGLDSSS
                     VVACILRQPDAPVDLPTFSALFPGFEKDESAYIGLLTRHFSLSNYPTTPTATDLVRDF
                     ERLVHHQEEPFLSSSIYAQFNVYGLAARHSTKVLLDGQGADELLAGYHKYYNWYWQEL
                     YRSDKKAFALELEAARESGVSDRWTWKNRLAAHLPVYAGLFLKKKRTAQQRRSSDLSR
                     DFIREFGESYYDIPHIDKLSGILYYNTFMNGMEELLRYADRNSMAHGIEIRLPFLDHN
                     LVEFVFSLPSHFKIREGWTKWLLRRSMEDLLPAEIVYRRDKTGFEPPQEDWMQDPTLQ
                     EYIHEARRNLVKAGILSPRVLHKKIQ"
     gene            complement(1834..2403)
                     /locus_tag="VKQ52_19195"
     CDS             complement(1834..2403)
                     /locus_tag="VKQ52_19195"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HLZ89393.1"
                     /translation="MKKLVLFVSMILGITLAKAQPPAAPSNLDSSPLDMSYYPVDYPV
                     LKIQDKVAEPLVARVVYSRPQKRGRKLFGDLIGYGQVWRLGANEATEIEFFRDVRIDD
                     KPVKKGRYTLYALVNEDKWILILNKETDTWGAFRYDSSKDVLRTAVTVEKQSDIAEAF
                     SIYFQKAGKGAADLRICWDDVLVKLPIRW"
     gene            complement(2413..3018)
                     /locus_tag="VKQ52_19200"
     CDS             complement(2413..3018)
                     /locus_tag="VKQ52_19200"
                     /inference="COORDINATES: protein motif:HMM:NF016239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HLZ89394.1"
                     /translation="MNYLAHAWLSFGIPEITVGNLISDFVKGKQKESYPVKIRQGIML
                     HRAIDGFTDIHPVTRQAKSWFRSAYGLYSGPLTDIVYDHFLANDPLIFPPGDAGSPLK
                     AFAQKTYQQVDEWQAILPERFARLYRYMRTEDWLGNYGHKELIFKSFEGLARRAKYMP
                     GAEEAKRLFEAHYSDLEACYVDFFPELKDFTLRTLEQVQSI"
BASE COUNT          780 a          853 c          963 g          839 t
ORIGIN      
        1 ctgaattttt ttgtgtagta cacggggtga gaggatgccg gccttcacca ggttgcgtct
       61 tgcctcgtgg atatattcct gcagggtcgg gtcctgcatc cagtcctcct gcggcggctc
      121 aaagccggtt ttatcccttc gataaacgat ctccgccgga aggagatctt ccatgcttct
      181 gcggagtaac cattttgtcc agccctccct gatcttgaaa tgcgagggga gggagaaaac
      241 gaattcaact aaattatgat ccaggaaagg aagtcttatt tcgatgccat gggccatgga
      301 attgcggtca gcatagcgta aaagctcttc cattccgttc ataaacgtgt tgtaatagag
      361 aattccactc aatttatcga tatgtgggat atcataatac gactcgccga actcccggat
      421 gaaatcgcgg gacagatcgc tgctccggcg ttgctgcgcc gttcgtttct tcttcaggaa
      481 gaggcccgcg tagaccggca ggtgggctgc caggcggttc ttccatgtcc agcgatcgct
      541 gactcccgat tcgcgggcgg cctcgagctc gagggcgaag gcctttttat cgctgcggta
      601 tagctcctgc cagtaccagt tatagtattt atgatagccc gcaagtaatt cgtctgcccc
      661 ctgcccgtcc agcagcactt ttgtggaatg ccgggctgcc aggccgtata cgttgaactg
      721 ggcatagata ctcgaggaga gaaagggttc ttcctggtgg tggacgagcc gttcaaaatc
      781 gcggaccagg tcggttgccg tgggtgttgt cggatagttg gacagggaga aatgccgggt
      841 gagcaggccg atataggcgg actcgtcttt ttcaaaaccg gggaagaggg cggagaacgt
      901 gggcagatcg accggggcat ccggctggcg cagaatacag gccacaaccg aagagctgtc
      961 caatccgccg ctaaggctcg ttcccacgcg gacatcgctg cggagccgcc ggtggaccga
     1021 ggcgtggagc agctggcaaa aggtgtcgac ggcggtctct tcttttatcg gcgccgtttg
     1081 tcctgcctcg atatcccagt atacggcggt ttcgagatgg cccgtcgcgg gttgatagat
     1141 aagaaaggac cgggccggga gtttggaaat gccctcgaaa aaggtctcaa aactattgcc
     1201 ggggttttgg gtatagccga tggtcaggaa gttaaagagc atcttcgcat ttggctcttt
     1261 ctttatcccg gcggcccaaa gggcctttgc ttcgctcgcg aaaagaaatt ggtcggtatc
     1321 cctgtaaaag aacaggggtt tctcgccgaa gcggtcacgg gcaagaaaaa gctgttgttt
     1381 ggtttcatcc cagatggcga aagcaaacat cccgtcgaag tactggagac agtcgacgcc
     1441 atagtgatcg aaggcggcga cgatgacctc ggtatccgtg cgggaggtga aggcatatcc
     1501 tttcgattgc agtacgctac gcagttcgag gtaattatac aactcaccgt tgtggatgat
     1561 cgtatagcgg tcgaggtaat gcatcggctg gtcgccggcc gggctgagat cgatgatcga
     1621 gagccgccgg tggccgaggc cagcgaggcc ggagcggctg agccagatgc cttccccgtc
     1681 cgggccgcga tgggcgatag cggccgtcat attcttcagc cgctgtggag ttattttcgc
     1741 cgcatcggca gatagaatgc ctgcaatacc gcacataggt tgtgatttag ccgatctggc
     1801 cgatcgtggc cataccgggg cgttttcggc gccttaccag cgtatcggga gttttacgag
     1861 cacatcgtcc cagcagatcc gcaggtcggc ggcgcctttt cctgcttttt gaaagtagat
     1921 ggagaaggct tctgcaatat cggattgttt ttctaccgtg acggcggtgc gcaaaacatc
     1981 tttggaagaa tcgtagcgga aggcgcccca ggtgtccgtt tctttattca ggatcaggat
     2041 ccatttatcc tcgttgacaa gcgcatacag ggtataccgg ccctttttga ccggtttgtc
     2101 gtcgatcctg acatccctga agaactcgat ctccgtggct tcgttggcgc cgagccgcca
     2161 tacctgcccg tagccgatca gatcgccgaa cagcttcctg cccctttttt gcggccggct
     2221 gtagaccacc cgggcgacaa ggggttcggc gaccttatcc tggatcttca gaacgggata
     2281 gtctaccggg taataactca tatcgagtgg ggaactgtcc agattggaag gtgcggccgg
     2341 tggttgggcc tttgccaggg tgatacccag gatcatactc acgaatagaa caagcttctt
     2401 cataggccaa aattaaatac tttgaacctg ttccaacgtc cgcagggtaa aatctttcaa
     2461 ctccggaaaa aaatccacgt aacaggcttc caggtcgcta taatgggctt cgaaaagccg
     2521 ctttgcctcc tccgcgccgg gcatgtattt tgcccgcctg gccagtcctt caaagctctt
     2581 aaaaataagt tccttatgcc catagttgcc aagccagtcc tctgtccgca tatagcggta
     2641 taaccgggcg aaccgttcgg gcaggatggc ctgccattcg tccacctgct ggtaggtctt
     2701 ttgcgcaaag gcttttaggg gagagccggc atcgccggga gggaagataa gcgggtcgtt
     2761 ggcgaggaag tggtcgtaaa cgatatcggt gagcgggccc gaatagagcc cgtaggccga
     2821 tctgaaccag gattttgcct gccgggtgac ggggtggata tcggtgaacc cgtcgatcgc
     2881 gcggtgcagc atgatcccct gtctgatctt caccggatag gattccttct gctttccttt
     2941 tacaaaatca ctgatcaggt tgcctaccgt tatttccggt attccaaacg aaagccaggc
     3001 atgtgcgagg tagttcatct ctacaattta tcgattttct accgaagaat gaatttaaca
     3061 ggatgaaaga cgattttaaa catttattaa gaaatcgttg caagtgtacg cggggcgcgg
     3121 atttgagcga aaacataaaa accggtatcc ttaccgtgga acctttttgt ttaccattcg
     3181 ataacatggc gcgccattgg tccatattgg cttgcgtgtc gatgtaacgt atgatagctt
     3241 tgctgcgtct tagtgatagt tctttaacaa cgccacgaaa aaaaaatttt tgatcagctg
     3301 caacatggtt caaaaacctg cgtcttatta atagagcaaa atagagctcg caaacagaaa
     3361 agactttaaa aacttataaa aacgttagtt atgaaaaaga tcgtcttatc catcgctacc
     3421 atgcttatga tgggt
//
LOCUS       DATGFG010001011         3850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1081688, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001011 DATGFG010000000
VERSION     DATGFG010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081688"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VKQ52_19205"
     CDS             complement(<1..671)
                     /locus_tag="VKQ52_19205"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ89395.1"
                     /translation="MRQPDLVAFAVLLATLLLFARPLSAQPSGAHRISGKITSQTTGS
                     PVIGATITVKGTKNAVQASDNGDFVIMAAAGERLIITNVGFLPREVVVTNAAEIDIAL
                     RQDYNNLNDVVVVGYGKMKKTDLSSSQVTVTSEELHRTVNSTIEQALQGKAANVYVAS
                     SNAQPGAAPSVVIRGISSLSQGTQPLYVIDGVQIKPSDPQGGAAGSYNAPMSYASPLA
                     GLNPD"
     gene            894..>3850
                     /locus_tag="VKQ52_19210"
     CDS             894..>3850
                     /locus_tag="VKQ52_19210"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ89396.1"
                     /translation="MRPRKTLLSLLCCFCCLLYGRLWAQPQDYLFSHLTVTAGLSNNH
                     ITGIYKDNRGFMWFGTVAGLNRYDGYQFRQYRHDPRDPHTIADNYIEQIFEGPGGKMW
                     VESRTGRFNIYDFDQDRFDVDFGAYLRKLGLPTYQLVKITPSGKGYWFVYRDSGVYHY
                     LPDGKIVAFQPAPTPVTAVQEDGNGDCWVIHQDGLLEKIDGRQGQITFRTIALQKELG
                     NTPIPYTVFIDGQNDLWFFSNGVFKGTYFFHPSTGEWKHLAVDAGSRSLNSNMVLAAL
                     QDRNGRIWLATDHGGVDIVDKRDFSVKFISHVDDDAKSIAANSVTSLFRDNMGTVWLG
                     TFKNGLSYYHQNDLQFPQYRHEPGKAATLPYDDVNSFAEDASGNVWIGGNGGGLIYFD
                     RKKNSFRQWVHDPNNDNSLCSNIIVSLLLDREGKLWIGTYFGGLDCFDGHRFIHYRHR
                     EDDPASLADDRVMCLCEDFDRQIWVGTLAGGLDRLDRHSKRFFHYKSTLPNSISNDYI
                     STIITDGEGNVWVGTGYGLEFIERNTGNIRHFAPEPSRLSSNNVNWLFMDSRNRLWAG
                     TREGLDVLNPDGKTFRSFTTEDGLPDNTVRYIAEDSLRRLWISTANGLSRITVVESQN
                     RSALTLRCRNFHEQDGLQGREFNERAGLATKDGHLLFGGPNGFNIFRPQDFAPERRTP
                     PIVLTGLEVFDKSVHVGDTMNGHVILQKSLSESGDITLKYKENVFSIEFASLEYVPLA
                     TNRYAYMLDGFSHNWLITDGKIRKATYTNLDAGSYVFRVKASDADGEWYDQQATLRIV
                     VLPPWWKTPLAYVVYVLLLVGSLVFARHMVLQRARMRFALENERRETRRMHEMDLMKI
                     RFFTNMSHELRTPLSLILAPVDKLLDRGPQQDPRRSYEMIRRNAKRLLHLVNQLLDFR
                     KMEVNELKLHLREGDVVRFIKETAHSFVDLAEKKNISFVFDDGGCGMLVTHFDHDKLE
                     RILFNLLSNAFKFT"
BASE COUNT          802 a          944 c         1230 g          874 t
ORIGIN      
        1 tcgtcggggt tgaggccggc cagggggctg gcataagaca tcggggcgtt ataggagccg
       61 gccgcaccgc cctgggggtc ggagggtttt atctggaccc cgtcgatgac atataggggt
      121 tgagtgccct ggctcaggga actgatcccg cggatgacga cgcttggggc ggcgccgggc
      181 tgggcgttac tggaggcgac atagacattc gccgcctttc cctggagggc ctgttcgatg
      241 gtggagttca cggtgcgatg gagctcttcc gaggtgacgg tgacctggct gctgctcagg
      301 tcggtcttct tcatcttacc gtaccccacg accacgacgt cattcagatt gttataatcc
      361 tggcggagcg cgatatcgat ctcggccgca ttggtgacca cgacttcccg gggtaagaaa
      421 cctacattag tgataatcag tcgttcaccc gccgccgcca taatgacgaa gtccccgtta
      481 tccgaggctt gcacggcatt tttggtgcct tttacggtga tggtggcgcc gatgacggga
      541 gagccggtcg tttgggaggt gatcttaccg ctgatgcggt gagcgcccga aggctgggca
      601 gagagcgggc gggcgaagag caggagggta gccagcaata ctgcgaaagc caccaggtct
      661 ggttgtctca attttttcat acgcaatgat gcaatcggtt tatattacgg ggtaaaacta
      721 gtaacaatgc tgctggtgca ggggtatggt ttcggtcaaa tagggggtca aaatgtttaa
      781 aattgggggg ttttaagtcg aggcaaccca ttttattccc cttcgtattg tctatatttg
      841 taatagaacg aagacgactg cttttgaacg ttttgccatg tgaccattat tatatgagac
      901 ccaggaagac ccttctttct cttttgtgtt gtttttgctg tttactgtat ggacggcttt
      961 gggctcagcc ccaggactat cttttttccc acctgaccgt taccgcgggt ttgtcgaata
     1021 accatattac gggtatctat aaggacaacc gggggtttat gtggtttgga acggtggccg
     1081 ggttgaaccg gtatgacggg taccagttcc ggcagtaccg gcacgatccc cgtgatccgc
     1141 ataccattgc cgacaattac atagaacaga tcttcgaagg ccccgggggg aagatgtggg
     1201 tggagtcccg gacggggcgt tttaatatct atgacttcga ccaggaccgg tttgacgtcg
     1261 attttggcgc ctacctgcgg aagctggggc tgccgactta ccagttggtg aagatcacac
     1321 cctccgggaa gggttactgg ttcgtttacc gggacagcgg ggtttatcac tatttgccgg
     1381 acggaaagat cgtcgccttc cagccggccc cgaccccggt gacggccgtc caggaggacg
     1441 gaaatgggga ctgctgggtg atccaccagg acgggctgct ggagaagatc gatggacggc
     1501 agggccagat caccttcaga accattgcct tgcaaaaaga gcttgggaat acccccatcc
     1561 cgtacaccgt ctttatcgac ggccagaacg atctgtggtt cttctccaat ggggtcttta
     1621 agggaacgta tttcttccat ccgtctaccg gggaatggaa gcacctcgcc gtcgacgccg
     1681 gcagtcgcag cctgaacagc aatatggtcc ttgcggctct gcaggaccgc aacgggcgga
     1741 tctggctggc tacggaccat ggcggggtcg acattgtaga caagcgggat ttctcggtga
     1801 aattcatcag ccatgtggac gacgatgcca agagcattgc ggcgaacagt gttacttccc
     1861 tgttcaggga taatatgggt actgtgtggc tgggtacctt caagaacggg ctcagctatt
     1921 atcaccagaa tgatcttcaa tttccgcaat accgccacga gcccgggaag gcggctaccc
     1981 tcccctatga tgatgtcaac agttttgccg aggatgcgtc ggggaatgtc tggatcgggg
     2041 gtaatggcgg ggggctgatc tatttcgacc ggaagaagaa cagcttccgg cagtgggtgc
     2101 atgatcccaa caatgacaac agtttatgta gtaatatcat cgttagcctc ctgctggaca
     2161 gggaggggaa gctatggatc ggcacctatt ttggcggact cgactgtttt gacggccacc
     2221 ggttcattca ttaccggcat cgcgaggatg atcctgccag cctggccgac gaccgggtga
     2281 tgtgcctttg cgaggacttc gaccggcaga tctgggtggg gacccttgcc ggcgggctgg
     2341 accggctgga ccggcacagc aagcggttct tccattataa gagtacgctc cccaattcga
     2401 tcagcaacga ctatatttcg acgatcatca ccgatggaga ggggaatgtc tgggtgggga
     2461 cggggtatgg gttggaattc atcgaacgga acacgggcaa tatccgccat ttcgcgccgg
     2521 agccgagccg cctgagcagc aataatgtta actggctttt catggacagc cggaaccggc
     2581 tctgggcggg tacgcgggag gggctggatg tgctcaaccc ggatgggaag accttccgga
     2641 gctttaccac cgaggatggg ttgccggaca atacggtgcg gtatatcgcc gaggacagtt
     2701 tacggcggct ttggatcagc accgccaacg ggctttcgcg gataacggtg gtggagtccc
     2761 agaaccggtc ggcgctgacg cttcgctgcc ggaatttcca tgagcaggat ggattgcagg
     2821 gacgggagtt caatgagcgg gcggggctgg cgacaaagga tggtcacctg ctgtttgggg
     2881 ggcccaacgg cttcaatatc ttccggccgc aggatttcgc gccggagcgg cggacgccgc
     2941 cgatcgtgct gacgggcctg gaggtgttcg ataagagcgt gcatgtgggt gatacgatga
     3001 acggccatgt catcctgcaa aagtcgcttt cggagtcggg cgatatcacc ctgaaatata
     3061 aggagaatgt tttttcgatc gagtttgcct cgctggagta tgttccacta gcgacgaacc
     3121 ggtatgccta tatgctggat gggtttagcc acaactggct gatcacggac ggaaagatcc
     3181 ggaaggcgac gtataccaac ctggacgcgg ggagctatgt gttccgggta aaggcctcgg
     3241 acgcggatgg ggaatggtat gaccagcagg cgacgctgcg gatcgtggtg ttgccgccgt
     3301 ggtggaagac gccccttgcg tatgtcgtgt atgtgctgtt gctggtggga agtcttgtat
     3361 tcgcccggca tatggtgttg cagcgggcgc ggatgcggtt tgccctggag aacgaacggc
     3421 gggagacaag gcggatgcac gagatggacc tgatgaagat ccggttcttc accaatatga
     3481 gccacgagtt gcggacgccc ctgtcgctga tcctggctcc cgtggacaaa ttactggatc
     3541 gcggtccgca gcaggacccg cggcggagtt atgagatgat ccggcgaaat gccaagcggc
     3601 tgctccacct ggtgaaccag ttgctggact tccggaagat ggaggtcaac gaactgaagc
     3661 tgcacctgcg ggaaggggat gtggtgcggt tcataaagga gacggcccac agctttgtcg
     3721 atctggcgga gaaaaagaat atcagctttg tctttgatga tggaggctgc gggatgctgg
     3781 tgacccattt cgatcatgac aaactggagc ggatactgtt caacctgttg tcgaatgcgt
     3841 tcaagttcac
//
LOCUS       DATGFG010001012         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_286496, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001012 DATGFG010000000
VERSION     DATGFG010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286496"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /locus_tag="VKQ52_19215"
     CDS             <1..1942
                     /locus_tag="VKQ52_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89397.1"
                     /translation="APIEFRQSWTRLLGELPPLPTGTNEAGEPILLPYTGPQTAKSSN
                     LENPELYAVYPYRLYDLQKPELELARNTFLHRRFRDKGCWEQDPIQAALLGFTDIARE
                     YTAFNFSRKDPALKFPAFWATGHDYLPDEDNGGNGENALQQMLLQSEGRKILLLPAWP
                     DNWDADFQLHAPFQTTIRGRVVRGKLTQLVVTPASRQADVIVKQDPVPFADNKTSWRD
                     GYDRFDYLMDEQTAEITPILATSEEGFGARPAEKGKIRCIVVAPKKAAPGNPWSWRGC
                     YWDHEPQADVELLRRGFYIGFIMCDPDRHWDAWYAFLTERHGLAKKPAFSGMSRGGIN
                     EFAWATTHPDKVACIYADNPALRPESFARLDALARHDVPLLHICGSYDFLLREHTLKV
                     EDYYHQLGGRISVLIKEGAAHHPHCLRDPKLIADWVENNMRPDESKPPAIPGANWDKS
                     YYYSYADTLLLLHPEELYATGRGPLFTVCYDRYDEDRGSNLDITGMTIVLPKKPAPGK
                     PWVFRAQRIWRWAEPVDLALLARGFTIVAAPVTAQAGPLTEQWDEVYRQMTMLGYSPK
                     PVLEGAGAAGGEAYYWAIANPDKLSCIYAQNPLLRSLRAKAPLTDSLAPLTKAGIPQL
                     NTRGALPADVTPIVDFILANTK"
     gene            1949..>2150
                     /locus_tag="VKQ52_19220"
     CDS             1949..>2150
                     /locus_tag="VKQ52_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89398.1"
                     /translation="MRHYLIPLLIAITGSLSTQAQMLAGTAKINITPATVEPIHDSVF
                     ARALVLESRGVKIAIVSVDLAVF"
BASE COUNT          444 a          665 c          625 g          416 t
ORIGIN      
        1 ggcccccatc gaattccggc aaagctggac gaggctgctg ggcgagttgc cgccgctgcc
       61 caccgggacg aacgaagccg gcgagcccat tctcttacct tataccgggc cgcagacggc
      121 caaatcctcc aacctggaga atcccgagct gtacgccgtc tatccctacc gtctgtatga
      181 tctgcaaaag ccggagctgg agcttgcccg caatactttc cttcaccgcc ggttcaggga
      241 caaggggtgc tgggagcagg accccatcca ggcggctctg ctcgggttca cggatattgc
      301 cagggaatac acggccttca acttttcgcg caaggacccg gctctgaaat tccctgcctt
      361 ctgggctacc ggtcacgatt atctgccgga tgaagacaat ggcggcaacg gggagaatgc
      421 gctgcagcaa atgttgctcc aatccgaagg gcgcaagatc ctgctgttac cggcgtggcc
      481 ggataactgg gatgccgact tccaattgca tgcgcccttc caaaccacga tacggggcag
      541 ggtcgtcagg ggcaagctta cccagcttgt ggtgacgcct gcttcgcgcc aggccgatgt
      601 catcgtaaag caggaccccg tcccctttgc ggataacaag accagctggc gcgatggata
      661 tgatcgtttc gactacctca tggacgaaca aacggcggag atcactccca tccttgcaac
      721 atccgaggaa ggattcggcg cccggccggc cgaaaaggga aagatccgct gtatcgtcgt
      781 cgcgccgaaa aaggcggctc cgggcaatcc ctggtcctgg cggggatgct attgggatca
      841 cgagccgcag gcggatgtgg agctgctgcg tcgcggcttt tacatcggct tcattatgtg
      901 cgaccccgac cggcattggg atgcctggta tgcctttctc accgaaaggc acggtctggc
      961 gaaaaagccc gctttcagcg gcatgagcag gggcggcatc aacgagttcg cctgggccac
     1021 cacccatccg gataaggtgg cctgtatcta tgccgacaac ccggcgctcc ggccggagag
     1081 ctttgcccgg ctggatgcgc ttgcgcgtca tgacgttccc ttattgcata tctgcggcag
     1141 ctatgatttc ctgctgcggg agcacacgct gaaggtggaa gactactatc atcagttggg
     1201 aggccgcatc tccgtgctca tcaaggaagg ggcggcccat catccgcatt gcctgcgcga
     1261 tccgaagctg atcgccgact gggtggagaa caatatgcgg cctgatgaaa gcaaaccgcc
     1321 cgccatcccg ggagccaatt gggacaagtc ctattattac agctatgccg acacgctgct
     1381 cctccttcat cccgaggaat tgtatgccac cggccggggt ccgctattta cggtgtgcta
     1441 tgaccgttat gatgaagacc ggggttccaa cctggacatc accggcatga cgatcgtcct
     1501 tccgaagaag cccgcacccg gcaaaccctg ggtgttccgg gcgcaacgga tctggcgatg
     1561 ggcggagccg gtggacctgg cgctcctggc caggggattt accatcgtgg cggctcccgt
     1621 gaccgcacag gccggtccgc tgaccgaaca atgggatgag gtataccggc aaatgacgat
     1681 gttgggctat tccccgaagc ctgtactcga aggagcgggc gccgccggcg gagaagccta
     1741 ctactgggcg atcgccaacc ccgataaatt gtcctgcatc tatgcgcaga accccttgct
     1801 ccgcagcctc agggcaaaag cgccactcac cgatagcctg gcgccattga cgaaggcggg
     1861 catcccgcag ttgaataccc gtggcgccct gcccgcggac gtcaccccga tagtcgattt
     1921 tatcctggcc aatacaaaat gatcttctat gcgtcactat cttatccccc tgctgatagc
     1981 cataaccggc agtctctcca cacaggcaca aatgctcgcc ggcacggcaa aaatcaatat
     2041 tacgccggcc acggtggaac cgattcatga ctctgtcttc gcccgcgcgt tggtgctgga
     2101 gagccgggga gtaaagatcg ccatcgtctc cgtagacctc gccgtgttca
//
LOCUS       DATGFG010001013         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_514842, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001013 DATGFG010000000
VERSION     DATGFG010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514842"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2528)
                     /locus_tag="VKQ52_19225"
     CDS             complement(<1..2528)
                     /locus_tag="VKQ52_19225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ89399.1"
                     /translation="MKNFFVSYKNPLTVTLVLVILGGMFAYSRLQTSLFPDVTFPKIK
                     VIADAGLQPVSKMMVTVTKPLENAIKRVPDLQYVRSTTSRGSCEISAFMDWKADIDLS
                     QQRIESRINEIKNDLPAETQITVEKMNPSILPVMGYTLESHDRSAIELKLLANYTVKP
                     FLSQVAGVSEVRIIGGRTKEYWVTLDAQKMSTLSITPDMLNTTIGQTGFVKSNGYLSD
                     YHFLYLTVTDATVHSKEDIGNIVVRNTGKRVVLLKDIAAVDIREAVEYTRINANGKDA
                     VLIAVIKQPTANLIDMSGSMEAKVADLQKVLPRGVTIRPYYIQADFVNTSVKSVTDSL
                     WIGLALAIIVAIIFLRSLKASATILITIPLTLCLTLIVLYAIGYTFNIMTLGAIAAAI
                     GLIIDDAIVVVEQIHRTHEEHPDESSVTLVQKAIHYLFPAMLGSSISTIVIFVPFVLM
                     SGVAGAYFKVLTNTMIITLVCSFLVTWIGLPVVYLLLTREGKQKRQVKPHNVKNQRWV
                     AFFIRRPWLSVIIMGALAWLIWFIVPQLETGFLPEMDEGSIVLDYNSPPGTTLQETDR
                     MLREVEKIIVATPEVQAYSRRTGTEMGFFITEPNRGDYLIQLKGDRDKSTEDVISDLR
                     KKIEATQPGLRIDFGQVIGDMLGDLMTSVQPIELKIFGDDQAKLHDLSKQVADLVSKV
                     EGTADVFNGIVIAGPSVSIEPNYARLAQFGLTPAALQYQLQTSLEGNVVGTLPEKEQL
                     SNIRMVYPGSRTISVADIGRLQIFLPDGKLKPINDLATVTIKAGDAEIQRENLQSMGV
                     VTGGLEGRDLGSVMKDIQQEVGSKIVLPPGYHITYGGAYAGQQQS"
     gene            complement(2525..3526)
                     /locus_tag="VKQ52_19230"
     CDS             complement(2525..3526)
                     /locus_tag="VKQ52_19230"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ89400.1"
                     /translation="MNTRLNGRRWVSIALAATILTACGALGKDGAGKADDKKDSAASE
                     KPAAAAADSVDAGTPVTVATVDRGGLDDYIELNAVSTFLQKSYVKAIANGYLQSADVY
                     PGKYVDAGQVLFTMKTKEAQTIGNAIEKLDTTLRFSGTSSIRANQHGYITQLNHQSGD
                     YVQDGEQLAVISDRSSFVFLLDLPYELRPFVLGKKNVELSLPDGTKLNGTLGPVMPTV
                     DSASQTQSVVIRIASSSAIPENLIARVRIVKSAKVNTQSLPKAALLTDETESDFWVMK
                     LIDSATAVKVPVKKGIETKDRVEILSPVFAKEDKILVTGNYGLPDTAKVKVETGGNP"
     gene            complement(3523..>4536)
                     /locus_tag="VKQ52_19235"
     CDS             complement(3523..>4536)
                     /locus_tag="VKQ52_19235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ89401.1"
                     /translation="VNGWGYDQAISNGGNFTTVVAVNQTLVGQKHLNAQYETVGLQNQ
                     GVRNTAKVSEQDLKKSVTAQYITTYGDLQQLAFNREVYALLQKEEVLLKDLTQKNVYR
                     QTDYLTFLVTLKQEGLLLRQLEIQFRNDHGMLNYLCGIVDTSATMLDDPHIELQALPE
                     ISNSVFFRQFHLDSLKLRNSRSLIDFGYRPKASLYADGGYSSTFAYQGYKNFGMSFGV
                     TVTMPIYDGHQRKMQYSKLEIAERTRTGYKDFFTRQYSQQIAQLRQQLEATEDLIGQI
                     NDQVKYSQALIDVNGKLLGTGDAKIADYIIALSNYLNAKNLLTQNNITRLQIINQINY
                     WNR"
BASE COUNT          936 a         1421 c         1152 g         1027 t
ORIGIN      
        1 gattgctgct gaccggcata agctccccca tacgttatat gataaccagg cggcaatacg
       61 atcttcgacc caacctcctg ctggatatcc ttcatcacac tacccaggtc gcgcccttcg
      121 agcccgcccg tgacgacacc catggattgc aggttctccc gctggatctc cgcatccccg
      181 gccttgatgg tcacggtggc aagatcgttg atgggcttca gcttcccatc cggcaggaag
      241 atctgcagcc ggccgatgtc ggccacactg atcgtccggc tcccggggta taccatccgg
      301 atattggaca gctgctcctt ttccggaagc gtgcccacga cattgccctc gagagaggtc
      361 tgcaactggt attgcagggc cgccggcgtc agcccgaact gcgccagccg ggcataattc
      421 ggctcgatgc tcacggacgg accggcaatg acgatgccgt tgaagacatc ggccgtgccc
      481 tccaccttcg agaccaggtc ggctacctgt ttggaaagat cgtgcagttt ggcctggtca
      541 tcgccgaaga tctttagttc gatcggctga accgaagtca tcagatcccc cagcatatcc
      601 ccgatcacct gcccgaaatc gatccgcaac cccggctgcg tggcctcgat cttcttacgc
      661 agatcgctga tcacatcctc cgtcgattta tcccggtcgc ctttcagctg tatcaggtag
      721 tccccccggt tgggctccgt aataaagaaa cccatctccg tcccggtgcg ccgcgaatag
      781 gcctgaacct cgggcgtcgc cacgatgatc ttctccactt cccgcagcat cctgtccgtc
      841 tcctgcagcg tggtgccggg aggggaatta taatccaata caatactgcc ctcatccatc
      901 tccgggagga aacccgtctc cagctgcggc acgataaacc agatcagcca ggccagggcg
      961 cccatgatga tgacgctcag ccagggacgc cggatgaaaa aagcgaccca gcgctggttc
     1021 ttcacattat gcggcttgac ctggcgtttc tgtttccctt ccctggttag cagcaggtac
     1081 accacgggca gaccgatcca ggtcacgagg aaggaacaca ccagcgtgat gatcatcgta
     1141 ttggtaagca ccttgaaata agcgcctgcc accccgctca tcagcacgaa cgggacaaag
     1201 atgacgatcg tgctgatcga agaacccagc atggccggga acaggtaatg gatcgccttt
     1261 tgcaccagcg tcaccgaaga ctcgtcagga tgctcctcgt gggtacggtg gatctgctcc
     1321 accaccacga tggcatcgtc gatgatcaac ccgatcgccg cggcaatggc gccaagcgtc
     1381 atgatattga acgtataccc gatggcatac agcacgatca gcgtcaggca aagcgtcagg
     1441 gggatcgtga tcaggatcgt tgcgctggcc ttgagactgc gtaaaaagat gatcgccaca
     1501 ataatggcaa gcgccagacc gatccaaaga ctgtcggtca cgctcttcac cgaagtattg
     1561 acaaaatccg cctggatata gtacggccgg atggtaaccc ccctgggtaa taccttctgc
     1621 agatccgcca ccttcgcctc catcgatccc gacatgtcga tcaggttggc ggtcggctgc
     1681 ttgataacgg cgataaggac ggcgtccttc ccgttggcgt tgatccgcgt atactccacc
     1741 gcctccctga tatccaccgc ggcgatgtcc tttaacaaca ccacccgctt ccccgtattg
     1801 cgaacgacaa tattgccgat atcctccttc gagtgaaccg ttgcatccgt taccgtcagg
     1861 tataaaaaat gatagtcgga gagatacccg ttggatttta cgaacccggt ctgcccgatc
     1921 gtcgtattga gcatatccgg cgtgatcgag agcgtgctca tcttctgggc atccagcgtg
     1981 acccagtatt ccttcgtcct gccgccgatg atcctgacct cggaaacccc ggccacctgc
     2041 gacaggaacg gcttgacggt ataattcgcc agcagcttga gctctatcgc cgaccggtca
     2101 tggctttcca gggtataccc catcaccggc agaatggaag gattcatttt ctcaaccgtg
     2161 atctgggtct ccgccggcag atcgttcttt atctcgttga tcctcgactc gatgcgctgc
     2221 tgactgagat cgatgtccgc cttccagtcc ataaaagcgg agatctcgca gctcccgcgg
     2281 ctggtggtgc tccggacata ctgcaggtcg ggtacccgct tgatcgcgtt ctccagcggc
     2341 ttggtgaccg tcaccatcat cttgctcacc ggctgaagac cggcatccgc gatcaccttt
     2401 atcttcggga aagtaacatc cgggaacagg gaggtctgca gcctgctgta ggcgaacatt
     2461 ccgcccagga tgaccagcac gagcgtaaca gtgagcgggt tcttgtagga aacaaagaaa
     2521 ttcttcatgg attacctccc gtctcaactt tcaccttcgc cgtatccggc aacccgtagt
     2581 tgcccgtcac caggatctta tcctccttcg caaacaccgg cgacaggatc tccaccctgt
     2641 ccttcgtctc gatacccttc tttaccggta ccttcaccgc ggtggcactg tcgataagct
     2701 tcatcaccca aaaatcactc tccgtctcat cggtgagcag ggccgccttt ggcagcgatt
     2761 gcgtgttgac cttcgccgac ttcacgatcc tcaccctggc gataagattc tccgggatgg
     2821 cggatgaaga agcgatccgg atcaccacac tctgcgtctg cgaagccgaa tccaccgtgg
     2881 gcatcaccgg cccgagcgtg ccgttcagtt tcgtcccgtc gggtaacgat agctccacat
     2941 tctttttccc cagcacaaac ggccgaagtt catagggaag gtccagcagg aatacaaagc
     3001 tgcttcgatc gctgatgacg gccagctgct ctccatcctg tacatagtcc ccggactggt
     3061 ggttgagctg cgtgatatac ccatgctggt tggcgcggat actgctcgtc cccgagaacc
     3121 gcagggtagt gtccagtttt tctatggcat tgccgatggt ctgcgcctcc tttgtcttca
     3181 tcgtgaacag cacctgcccc gcatcgacat atttgccggg gtaaacatcc gcgctctgca
     3241 aatagccatt ggcaatggcc tttacataac tcttctgcaa gaaggtggag acggcattca
     3301 gctcgatata atcatcgaga ccgccgcggt cgacggtggc gaccgtaacg ggcgtgcccg
     3361 catccacgga gtccgcggcc gctgccgccg gcttctccga ggcggccgaa tcttttttgt
     3421 catcggcctt gccggcgccg tccttcccca gcgcgccaca agccgtcaaa atcgttgccg
     3481 caagcgcgat acttacccaa cgccgtccat ttaaacgagt attcatcgat tccaataatt
     3541 gatctggtta atgatctgta gcctggtgat attgttctgc gtgagcaggt tcttcgcgtt
     3601 gagatagttg ctgagcgcga tgatataatc cgcaatcttc gcgtcacccg tccccagcag
     3661 cttcccgttc acatcgatca gcgcctgcga atatttcacc tggtcgttga tctgcccgat
     3721 caggtcctcc gtcgcctcca gctgctgccg cagctgcgcg atctgctggc tgtactgccg
     3781 ggtgaaaaaa tccttatacc ccgtccgcgt ccgctcggcg atctcgagtt tgctgtactg
     3841 catcttccgc tgatgaccgt catagatcgg catcgtcacc gtgacgccaa agctcatccc
     3901 aaaattctta tacccctggt aggcgaaagt ggatgaatag ccgccgtcag catacaggct
     3961 ggccttcggc cggtaaccga agtcgatgag cgaccggctg ttccgcagtt tcaggctgtc
     4021 caggtgaaat tgccggaaga acaccgaatt gctgatttcc ggcaacgcct gcaattcaat
     4081 gtgcggatca tccagcatcg tcgccgaggt atccacgatc ccgcagaggt agttcagcat
     4141 cccgtgatcg ttgcgaaact ggatctccaa ctgccgcaac aacagcccct cctgtttcag
     4201 ggtcacgagg aaggtgagat agtcggtctg ccggtagacg ttcttctgcg tcaggtcctt
     4261 taataacact tcctccttct gcagcagcgc atacacctcc cggttaaagg ccagctgctg
     4321 cagatcccca tacgtcgtta tatactgcgc ggtaacactc tttttgaggt cctgctcgga
     4381 aaccttggcc gtattccgca ctccctggtt ttgcaagccc actgtctcat actgcgcatt
     4441 cagatgcttt tgtcccacca gggtctggtt cacggccacc acggtcgtga aattgccccc
     4501 attggagatc gcttggtcat acccccatcc gttgac
//
LOCUS       DATGFG010001014         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1656066, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001014 DATGFG010000000
VERSION     DATGFG010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656066"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VKQ52_19240"
     CDS             <1..434
                     /locus_tag="VKQ52_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89402.1"
                     /translation="DSYDSFKGLVSEDIDWCKANGVDYVPLAFPGFSWKNMNGPQSTV
                     IPRNRGHFFWKQVAGAREAGAGMLYIAMFDEMNEGTSIFKCNTVDRLPLNDGGGFVGI
                     DTDLGSDYYLWLAGQAAAWWHGKGGYSSEMPRRPKSELPVP"
     gene            590..2464
                     /locus_tag="VKQ52_19245"
     CDS             590..2464
                     /locus_tag="VKQ52_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89403.1"
                     /translation="MLLAIDISSMIALNIILLLVYLILLATVLAILLFRKRNRLIRMI
                     VFSVTCILFSKTISDGIILPIHGAVKNIEYLTSLDADKISDKDAKDKWTPIYVMLEKF
                     PGANLLIAIAAGLSAFYITKLISEKVRSGSINLIAFLLSAALTIIFFRQAFQAAIITP
                     VSILAAREEVTLQNGESGGSLQGASTTIGEPSHSFPLSYVLEALGFGILCYLLLNYLF
                     KRKPLFSAPADGKAASVRLINGGILFILAFSVFLVVSVFVAIPILNEINRPSVYTPAK
                     LDSAFSLIANSRDSLIVRLNAPKKSDVSPLSDTSLNADFTKLLLHQRGTIYNQIQSVN
                     LEWGSIIRRRNEIIDRLNNYGQTYKSREATFRQGLVNSFDVSANNSIVNKGNLYEFAV
                     NRFISYRQTIRTIFENTDNDIALNDIFNENALRDIKLRLESTLSYMDSTNYQPIPVQT
                     YFVPYSTAFDQIEGQLAGDFSLQAENRSGREWGFLGVMSDYLIKTHSSELVLLIGMLG
                     FGLLGASLLSFAPRDENSNFIESLSVKPLINNFGGVLARGFGAALIVYLATKGGLALF
                     TLGGAASDPNGYILLLACFIGAVFSERVWTRISNAFGAKDAAEQGEKDKVVANQDRHS
                     "
     gene            complement(2482..>3362)
                     /locus_tag="VKQ52_19250"
     CDS             complement(2482..>3362)
                     /locus_tag="VKQ52_19250"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ89404.1"
                     /translation="TNIVNYTLRNNGAEAWRWMFGLGFIPSALFFLGALWLPESPRWL
                     IKAGRTAAADHVLRKIGGHAFATESLVSIQRSLGSGGRFNYRAVFQRAVLPAVLVGIG
                     LAVFQQLCGINVVFNYTALIFSSIGIPRDGQLLQTVFIGGVNLVFTLLAMVLVDRLGR
                     KPLMLFGAGGLCILYIIVVRMLGSGSPAVSAFLLTAIGVYALSLAPVTWVLISEIFPN
                     KVRGAATSVAVLSLWVAYFILIFTFPMLYEKWKDSTFYIYAVVCGLGFLFVWLSVKET
                     KGKTLEELEEVMVSHH"
BASE COUNT          826 a          852 c          900 g          784 t
ORIGIN      
        1 aggacagcta tgattcgttc aaggggttgg tttcggaaga tatcgactgg tgcaaggcta
       61 acggggtaga ctatgttccc ctggccttcc cggggttctc ctggaagaac atgaacggac
      121 cgcaatcgac cgtcattcca aggaaccggg gccatttctt ctggaagcag gtggcgggcg
      181 cgcgggaggc cggtgcgggc atgctgtata tcgccatgtt cgatgagatg aacgaaggga
      241 catcgatctt caagtgcaac acggtggacc ggcttccgct gaatgatggg ggtggttttg
      301 tcggcatcga taccgacctg ggcagtgact attatctctg gcttgccggg caggcggccg
      361 cgtggtggca tgggaaggga gggtatagca gcgagatgcc gcgccggccg aaatccgaac
      421 tacccgttcc gtaagctttt tgtggggttg gccactgccg tgattgttgc atctccggta
      481 gctggatcag tatggacagg tgtttgcgcg aaataaaggt gttgttacgc tagctaatcc
      541 aggtgaaaga tcgtagcttg ctcgtaaggc ttcgttaatg ccgtttgcca tgctgctagc
      601 tattgacatc agttcgatga tcgccctaaa catcatttta cttctggtat atctgatatt
      661 gctggcgact gtgctggcaa tactcctttt ccggaaaaga aacaggttga tacgaatgat
      721 cgtattttcc gtcacctgca ttctgttcag caaaactatt tcagatggga tcatcctgcc
      781 gatacatggg gcggttaaaa atatagaata tcttacatcg ctggatgccg ataaaatatc
      841 cgacaaagac gcaaaagata agtggacccc catttacgta atgctcgaaa agtttcccgg
      901 agccaattta ctgatagcga tcgcggccgg gctgtccgcg ttttacatta ccaaattgat
      961 aagcgaaaag gtgagatccg gttcgatcaa cctgatcgct tttctgctat ccgccgcact
     1021 gaccattatt tttttccggc aggcttttca ggcagccatc atcacccccg tctccattct
     1081 tgccgccagg gaggaagtta cgcttcaaaa cggggaaagc ggcggaagtc tccaaggagc
     1141 ttcgaccact atcggcgagc cgtcgcacag tttcccatta tcgtatgtgt tggaggcgtt
     1201 gggctttgga attctttgtt acctgttact aaattacctg tttaagagga agccgctctt
     1261 ctcagccccc gctgacggga aggcagcaag tgtacggctc atcaatgggg gtatactgtt
     1321 cattctggcc ttcagcgtgt tcctggtagt tagcgttttt gtagcgatac cgatactgaa
     1381 cgaaataaac agaccgtccg tttatacgcc ggccaaactg gactctgcgt tttcccttat
     1441 agccaatagc agggatagtt taatcgtcag gcttaacgca ccgaaaaaga gcgatgtatc
     1501 tcctctcagc gacacttccc tcaatgcaga tttcacaaaa cttttattgc atcagcgagg
     1561 caccatatac aatcagatac aatccgtgaa tttggaatgg ggaagcataa tccggcgcag
     1621 aaatgaaata atcgacaggc ttaacaatta cggacagacg tacaagagca gggaagctac
     1681 ctttcggcag gggctggtga acagctttga cgtcagtgcg aataactcga tcgtcaataa
     1741 aggaaacctc tatgaatttg cggtaaatcg ctttatctcc tatcggcaaa cgataagaac
     1801 gatatttgaa aacacggaca atgacattgc cctgaatgat atatttaacg agaacgcatt
     1861 acgcgacata aaacttcggc tggaatccac ccttagctat atggattcaa ccaactatca
     1921 acctatccct gtccaaacct attttgtacc gtactctacg gcgttcgatc aaatagaagg
     1981 gcaacttgcg ggcgatttca gcctgcaagc tgaaaacagg agcggaaggg aatggggatt
     2041 tcttggcgtg atgagcgatt atctgatcaa aacgcattcg tctgaattgg tattgctgat
     2101 cggcatgctt gggttcggct tgctgggcgc ctctttgctg tcgtttgcac ccagggatga
     2161 aaacagtaat tttatcgagt cgttgtctgt taaacccttg ataaacaact tcggcggcgt
     2221 gctggcacgc ggctttggcg cagcgctcat cgtatatctg gctaccaaag ggggacttgc
     2281 ccttttcacc ctggggggag ccgcgtccga tcctaatggc tatatccttc tattggcttg
     2341 ttttatcggc gccgtattca gcgaacgggt atggacacgt atcagtaacg cattcggcgc
     2401 aaaggatgca gcggagcagg gagagaaaga caaggtagtc gccaatcagg atcggcactc
     2461 ctgatgcctt agggtgcgca tttagtgatg acttaccatc acttcctcca actcctccag
     2521 ggtcttgcct tttgtttcct ttacgctcaa ccaaacgaac aggaatccaa ggccgcatac
     2581 aacggcgtag atgtaaaagg tgctgtcttt ccatttctca tatagcatcg ggaaggtaaa
     2641 gatcaggatg aaataagcca cccagaggct cagaacggcg actgaggtgg cggcgccacg
     2701 cactttattc gggaagatct cggagatcag cacccaggta accggcgcca acgacagcgc
     2761 atagacgccg atggccgtca gcaagaacgc cgacacggcg ggggagccgc tgcccagcat
     2821 ccggacgacg atgatgtaga ggatacaaag cccgccggcg ccgaacaaca tcaacggctt
     2881 ccggcccagc cggtctacca gtaccatggc cagcagggta aacaccaggt tgaccccgcc
     2941 gatgaacacg gtttgcagca gctgtccgtc ccggggtatt ccgatgctgc tgaagataag
     3001 cgctgtatag ttgaagacga cattgatgcc gcacaactgt tgaaacacgg ccagcccgat
     3061 gccgaccagc accgccggga gcacagcccg ctgaaatacc gccctgtagt tgaaccggcc
     3121 gcccgagccg agtgaccgct gaatgctgac cagcgactcg gtggcgaagg catgtccgcc
     3181 gatcttccgg agcacatggt ccgcggcggc tgtccgcccc gcttttatca accagcgtgg
     3241 gctctccggc agccagagcg ctcccaggaa aaacagcgcc gaaggaataa acccgaggcc
     3301 gaacatccag cgccaggctt cagcgccgtt gttgcgaagg gtatagttga cgatattggt
     3361 ga
//
LOCUS       DATGFG010001015         4929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1427171, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001015 DATGFG010000000
VERSION     DATGFG010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427171"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2711)
                     /gene="sprA"
                     /locus_tag="VKQ52_19255"
     CDS             complement(<1..2711)
                     /gene="sprA"
                     /locus_tag="VKQ52_19255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HLZ89405.1"
                     /translation="MTRHFFLLILATVVLTAASVVCCTAVSAHPFQQQRDTTAPGRRD
                     TIPHRTDTVGLADYYMSQMRRAADSIRRQRDSIRRLQGNVPAAAGNDTTQRPDSIRRQ
                     PAVRDTARPGDSLRFPLYDRRGDRFSYPNHNPFDLKDPPNLHDSIVYDPATNQYYILE
                     KIGNQYYRKPTYLTFEEMMKMSGDSSENDYFRKRADALDALNRRLLRPKLSVSDNLFN
                     RIFGSGPINIKPQGNVDITAGYMGQNIQNPTLPESARRTGGFDFNENANIQVVGNIGD
                     KLKLPISYNTLANFDFENQLKLDYTGGTDEIIKKVEAGNVSFTTPSTLMTGAQSLFGI
                     KTKMQFGKLSITAVLANQRSQKQTVSSQGGAAVTTFQFKADDYDENRHFLLAQYFRNN
                     FNKAMSSIPVVQSQVQILRVEVWVTNRNGIDTGSRQVVGLMDLGEASPYSNRIGRGTG
                     NGLPDNDANTEYRSIINDPTSRSPSTAANKLNALGLTQVQDFEIVYARKLNATDYYFN
                     PQVGFISVNQTLQANDVLAVAYQYSYNGHIYQVGEFSTDVPPDTTAGTGGGASKVLYL
                     KLLKATSQRTNLPLWNLMMKNVYSLKTATGSYLANIQQTGFQFNVNYDEPSKGTKRYL
                     PEGPKANIPLLTVLGLDRLNAHNDPGADGIFDYIEGLTVISNQGRIIFPVLEPFGRDL
                     DTLAFSGAPQSLKNKYVFYQLYDTIKAVAQTYANVDRYVLNGTAKGQSTTSLSLNAYN
                     VPPGSVVVTAGGQTLKENVDYVVDYNLGQVQIINQAIVNSGVPVNVSFENNANFGLQQ
                     RSFMGVRLDYLAKNTATESLNVGATFERLNERPFFSKTDYGEDPIRNTMFGTDVNYRG
                     QLPQLTRLLDKLPFYSTKEMSTINAYGEGAILRPGHPPQIGSGSSGAV"
     gene            complement(2754..3341)
                     /gene="ruvA"
                     /locus_tag="VKQ52_19260"
     CDS             complement(2754..3341)
                     /gene="ruvA"
                     /locus_tag="VKQ52_19260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HLZ89406.1"
                     /translation="MIAFLRGTFTFKSPALVHMDVAGVGYELQISLNTYSRIEPLESG
                     LLLTWLHIREDAHVLYGFFEKAEKDLFIQLLSVSGVGASTARMMLSSMKPEEITRAIL
                     QGNAKQLESIKGIGKKSAERIILELRDKLGKSREENINISSLTSNTLEQDALNALITL
                     GIARPAAEHAIKRVMSEGPGADKIEDIIKKALKIL"
     gene            3469..4461
                     /locus_tag="VKQ52_19265"
     CDS             3469..4461
                     /locus_tag="VKQ52_19265"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HLZ89407.1"
                     /translation="MSKKITAAITAIGGYVPEDRLTNADLEKMVDTNDEWIRTRTGIE
                     ERRILKGAGKATSDLCVPVVEEICRKRGIGPEEIDCVIVATVTPDMLFPSTANIVCDK
                     VGAKNAWGFDMSAACSGFLYALTSGAMYIESGRCRKVIVLGADKMSSIVDYTDRTTCI
                     IFGDGAGGVLLEPNTEGYGVLDSILRSDGSGRTHLHMKAGGSLRPSSFETVAAKEHYI
                     YQEGQAVFKFAVKGMADVSAELMERNKLTADDISWLVPHQANKRIIDATAHRMGLSEE
                     KVMINIQRYGNTTAGTIPLCIWDWEDKLNKGDNLVLAAFGGGFTWGATLIKWGH"
     gene            4483..4929
                     /locus_tag="VKQ52_19270"
     CDS             4483..4929
                     /locus_tag="VKQ52_19270"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ89408.1"
                     /translation="MENIAIRRAVRGDCPRLLELIHELAEYEKAPQEVTVTLEHFTES
                     GFGPQPVWWAFVATVDDRVAGFALYYIRYSTWKGQRLYLEDLIVTGEMRGKGLGKLLF
                     DRLIEEVREKNFTGMVWQVLDWNEPAINFYKKYNTQFDAGWINCSI"
BASE COUNT         1065 a         1228 c         1453 g         1183 t
ORIGIN      
        1 acggcgccgc tgctgcccga gcctatctgc ggcggatgac cgggccggag gatcgcgcct
       61 tcgccgtagg cgttgatcgt gctcatttcc tttgtcgagt agaacggcag tttgtccagc
      121 agacgggtaa gctggggaag ctggccgcga taattgacat ccgtgccgaa catcgtgttc
      181 cggatgggat cttcgccata gtcggtcttg ctgaagaagg ggcgttcgtt gaggcgttcg
      241 aatgtggcgc cgacgttgag ggattccgtt gccgtattct tggcgaggta atcgaggcga
      301 acccccataa agctcctctg ctgtaatcca aaattggcat tgttctcaaa ggatacattg
      361 acgggtacac ccgaattgac gatggcctgg ttgatgatct gcacctggcc cagattgtag
      421 tccaccacat agtctacatt ctccttcagg gtctggcccc ccgcggtgac cactaccgat
      481 cccggaggca cattgtaggc gttgagggaa aggctggtag tggattgtcc cttggcagtg
      541 ccattgagca cataccggtc gacgttggca taggtttgcg ccacggcctt tatcgtatca
      601 tacagctggt aaaagacata cttattcttt agtgactgtg gcgcacccga aaaagccagc
      661 gtgtcgaggt cgcggccaaa gggttccagc accgggaaga tgatgcgccc ctggttggaa
      721 atgacggtga gcccttcgat atagtcgaag atcccgtctg ccccggggtc gttatgggca
      781 ttcaaccggt cgaggcccag gacggtcagc agggggatat tggccttggg cccctccggc
      841 aggtatcgct tcgtaccctt gcttggttca tcgtagttga cattgaactg gaagcccgtc
      901 tgctggatat tcgccaggta gctgccggta gccgtcttca gggagtagac gttcttcatc
      961 atgagattcc agagcggcag gttggtccgc tgggaagtcg cctttaacaa cttaagatac
     1021 aacaccttgg atgctccgcc cccggtcccg gcggtggtat ccggcggcac atcggtagag
     1081 aactcaccca cctggtaaat atgaccgtta tagctgtatt gataagcgac ggccagtaca
     1141 tcgtttgcct gcagtgtctg attcacggaa ataaagccaa cctgggggtt gaaataataa
     1201 tcggtggcgt tcagcttccg ggcatacacg atctcaaaat cctgtacctg ggtcaggccc
     1261 agggcattga gtttatttgc cgccgttgag gggctccggc tggtggggtc gttgatgatg
     1321 ctccggtatt cggtgttggc gtcgttatca ggaaggccgt taccggtgcc ccggccgatc
     1381 cggttgctat agggggaggc ttcgccgagg tccatcaacc ctactacctg gcgggagccg
     1441 gtgtcgatcc cgttgcggtt ggttacccat acctcgacac gcaggatctg gacctgggac
     1501 tggacgacgg ggatgctcga catcgccttg ttaaaattat ttctaaagta ctgggccagc
     1561 aggaagtgcc ggttctcgtc gtagtcatcc gccttgaact gaaaggtggt gacggcggcc
     1621 ccaccctggg aggagacggt ctgtttctgc gagcgctggt tggcgagaac ggcagtgatc
     1681 gacagcttgc cgaactgcat cttggtcttg atcccgaaaa gcgactgggc tcccgtcatc
     1741 agggtgctgg gggtcgtgaa ggagacatta ccggcctcca cctttttgat gatctcgtcc
     1801 gtcccgcccg tataatccag ttttagttga ttttcaaagt caaagtttgc taaagtatta
     1861 tagctgatgg ggagtttcag tttgtccccg atgttaccga ctacctggat gttggcattc
     1921 tcgttaaaat caaagccacc ggtgcgccgg gcgctttccg ggagagtcgg gttctggata
     1981 ttctggccca tatagcccgc ggtgatatcg acgttgccct ggggtttgat atttatcggc
     2041 ccgctaccga aaatgcggtt gaaaaggttg tcgcttacgg agagctttgg tcggagaagc
     2101 cgccggttaa gcgcatcgag tgcatcggcc cgtttccgga aataatcatt ctcggacgag
     2161 tcccccgaca ttttcatcat ctcctcgaag gtgaggtagg ttggttttct ataatactga
     2221 ttaccaatct tttcgaggat ataatactga tttgtcgcgg gatcgtagac gatggagtca
     2281 tgcaggttgg gcgggtcttt caggtcgaag ggattatggt tggggtaaga aaagcggtcg
     2341 ccgcgccggt catagagggg aaagcgaaga ctgtcgccgg gccgggccgt gtccctgact
     2401 gcgggctggc gccggatact gtcgggacgc tgggtcgtgt cgtttccggc ggcggccggg
     2461 acattgcctt gcaaacggcg gatggaatcc cgctgccggc ggatggaatc ggcagccctt
     2521 cgcatctggc tcatgtagta atcggccagg cctaccgtgt ctgtcctgtg tgggatggta
     2581 tcccgccggc ccggggccgt tgtgtcccgt tgttgctgga atgggtgcgc ggaaacagct
     2641 gtacaacaca caactgaagc agccgtcaac actaccgttg ccaggatcaa cagaaagaaa
     2701 tggcgggtca aactttcctc aagaattagt taggtagagt tccagcgata ggcttacaag
     2761 atttttaaag ctttcttaat aatatcctcg attttatcag ctccgggccc ttcactcatg
     2821 actcttttga tggcatgttc tgcggcgggt ctggctatgc ccagcgtaat cagtgcattt
     2881 aacgcgtcct gttctaaggt attgcttgtc agcgatgaaa tatttatgtt ttcttccctt
     2941 gattttccca gcttgtcgcg tagttcgagg atgatccgtt cggccgattt tttgccgatg
     3001 cccttgatac tttccagctg ttttgcgttc ccctgcagga tggccctggt gatctcttcc
     3061 ggtttcatcg aggacaacat catccgggcg gtggacgcgc cgacgcccga tacgctgagg
     3121 agttggataa agaggtcttt ctccgctttc tcaaagaatc catagagaac gtgcgcgtct
     3181 tcacggatat gcaaccaggt aagcagcaac ccgctctcca gtggctcgat cctggaatag
     3241 gtgttgaggc ttatctgcag ctcatacccc acgccggcaa catccatgtg tacgagggcg
     3301 gggcttttga aggtgaaagt accacgtaaa aaggcgatca tggtcacgaa aataggctaa
     3361 attatgcgcc ggcgatgcct ctgcggcgac ttttatgtgc cgggccgcat ttgaatattt
     3421 ttccccacct ttgcaaaaat cttttcttaa cctgattgga atttaataat gagcaagaaa
     3481 atcactgccg ctatcaccgc gataggagga tacgtacccg aagaccgtct taccaatgcc
     3541 gacctggaaa aaatggtgga taccaacgat gaatggatac gtacacgcac ggggatagag
     3601 gaaagaagga tactgaaagg cgcaggtaaa gcaacttccg atctgtgtgt gccggtggtg
     3661 gaagagatct gccgcaaacg gggcatcggg ccggaagaga tcgattgtgt catcgtggcg
     3721 acggttaccc cggacatgtt attcccctct acagccaata tcgtctgtga caaggtgggt
     3781 gcgaagaatg cctggggctt tgatatgagc gccgcctgtt cgggattttt gtatgctctc
     3841 accagcggcg cgatgtatat cgagagcggc cgttgcagga aggtcattgt gcttggcgcc
     3901 gataagatga gcagcattgt cgattatacc gatcggacca cctgtatcat ttttggcgat
     3961 ggcgccggcg gggtattgct ggagcccaat acggagggtt atggagtgct ggacagcatc
     4021 ctcaggagtg atggcagcgg ccggacccat ctgcatatga aggccggcgg ctcgttacgg
     4081 ccatcctctt tcgaaaccgt tgccgcaaaa gagcactata tataccagga gggccaggcc
     4141 gtcttcaagt tcgcagtgaa agggatggcc gatgtaagtg ccgaactgat ggagcggaac
     4201 aaactgaccg ccgacgatat cagttggctc gtccctcacc aggccaacaa acgcatcatc
     4261 gacgcaacgg cgcaccgcat ggggctttcg gaagagaaag tgatgatcaa catccagcgg
     4321 tatggaaata ctaccgccgg aaccatcccg ctttgcatct gggattggga ggacaagctc
     4381 aacaaggggg acaacctcgt tctggcagcc tttggcggcg gtttcacctg gggcgccacc
     4441 ttgatcaaat ggggacactg aaccggacgg aaattcattc acatggaaaa tattgcgatc
     4501 agaagggcag tccgtgggga ctgcccacgt ttattggaat tgatccacga gttggcggaa
     4561 tatgagaaag ctccgcagga ggtgacagta accctggaac attttaccga aagcggtttc
     4621 gggcctcaac cggtctggtg ggcctttgtc gccaccgtcg acgaccgtgt cgccggattt
     4681 gccctgtatt atatccgtta cagcacctgg aaggggcaac ggctttacct ggaagatctt
     4741 atcgtcacgg gggaaatgcg cggcaagggg cttgggaagt tactgttcga tcgccttatc
     4801 gaagaggtgc gggaaaagaa tttcaccggc atggtatggc aggtgctgga ttggaatgaa
     4861 ccggcgatca atttctataa aaaatataac acgcaattcg atgcgggctg gatcaattgc
     4921 tccatctga
//
LOCUS       DATGFG010001016         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1443491, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001016 DATGFG010000000
VERSION     DATGFG010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443491"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..494)
                     /locus_tag="VKQ52_19275"
     CDS             complement(135..494)
                     /locus_tag="VKQ52_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89409.1"
                     /translation="MKKFLGITVVLIVVAVAIWFYFRFERPFGEGVKAGTLNYIVYKG
                     YLWKTYEGEVILAGFQNKPALQSNEFTFSIDNEAVAKRLELASGHEVQVHYLEYLGAL
                     PWRGYSKFIVDSVVSIR"
     gene            complement(506..1981)
                     /locus_tag="VKQ52_19280"
     CDS             complement(506..1981)
                     /locus_tag="VKQ52_19280"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLZ89410.1"
                     /translation="MVVGLAGGGRAFGQAAKEMAGETTGVTAGQKTGVTAGVTAGQTA
                     EDSALIRRLADTILTDGKAYGDLRELTKTVGARLSGSAGYYKAEKWGQAALTGAMADK
                     VWLQECMVPHWVRGGKDSARWNAGAPKTGTYNAHYVMTGGLDILALGNSVGTGPAGIT
                     APLVLIHNFDELEQRKDELKGKIVFYNYKFNPLFVETFQAYGDAVRYRGAGASRAAKY
                     GALAVLVRSMSSSVDNNPHTGALIYADSTQKIPAAAVGLRDADRLAALLEGPPRGVSV
                     YLRTNAQMLPDTLAHNVIGELRGSEFPDQYITVGGHLDSWDPAEGAMDDGAGCVQSIE
                     VLRALKAIGYKPRHTIRVVLFANEENGGRGGRAYAAAAKAAGEKHLLGLESDNGGFTP
                     RGFTLSLSEEKLAAMTPWLKLLKPYGVYAFEKGGGGSDVNPLGPQLGAVVGELRPDSQ
                     RYFDYHHARNDVLENVNKRELELGATNMAALVYLVDKYGLN"
     gene            2228..3355
                     /gene="bshA"
                     /locus_tag="VKQ52_19285"
     CDS             2228..3355
                     /gene="bshA"
                     /locus_tag="VKQ52_19285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HLZ89411.1"
                     /translation="MRIGIVCYPTFGGSGVLATELGKALADKGHQVHFITYQQPVRLN
                     EFNANIFYHEVRVTTYPLFDYPPYETALSSAMVDVINNHQLDLLHVHYAIPHASAAYM
                     AKKIVEAQGRYIPVITTLHGTDITLVGRDKTFAPVVAFSINQSDAITAVSQNLRDETF
                     RTFNIEKEIRVIHNFVDTRRFHRKPIDAFRRVIAPNGERILMHASNFRKIKRVQDIVR
                     IFEIVRQKIPSKLLFVGDGPERSEAEALCRELDIIDDTRFVGKQEQMEEILAVADLFL
                     LTSEYESFGLAALEAMAAGVPVVSTNAGGLPEIAIQGETGYLDDIGDIHGMSEHAIRI
                     LQDDETLKRFKANAAAHAKNFDIHRIVPQYEALYNRFLALA"
BASE COUNT          730 a         1239 c          820 g          687 t
ORIGIN      
        1 gagaattgcg cctgactaat gttaccacgt cttccggcaa gacctatcgg cttcttaacc
       61 taccctacta tctggcttcc caggtaaagg agtacattgc ctggctgaag tcagggacgc
      121 cgcgccggta aaacttatcg aatcgacacc acactatcta ctataaactt actatacccc
      181 ctccacggca aggccccgag gtattccagg taatgcacct gcacctcatg cccgctggcc
      241 agttcgagcc gctttgccac ggcctcgtta tcgatactaa aggtaaattc attgctttgc
      301 agggcaggct tgttctggaa gcccgcgagg atcacctccc cttcataggt cttccacaga
      361 tagcccttat agacgatata gttgagggtc cccgccttta ccccttcccc aaagggtcgc
      421 tcaaatcgga aatagaacca tatcgctaca gccaccacga tcaacaccac ggtgatgccc
      481 aggaattttt tcatactatg ccggtttagt tgagaccata cttatcgacg agatacacca
      541 gcgccgccat atttgtcgcg cccagctcca actcgcgctt gttcacattt tcgagcacgt
      601 cgtttcgcgc gtggtgatag tcgaagtagc gctgcgagtc cggccgcaac tcacccacca
      661 ccgcacccaa ctgcggcccc agcgggttca catcgcttcc cccgccgcct ttttcaaagg
      721 catacacgcc atagggtttc agcaatttca gccagggagt catcgccgcc agcttctcct
      781 cggacagcga gagggtaaag ccgcgcggcg taaacccgcc attatcgctt tccagcccca
      841 acaggtgctt ctctcccgcc gcctttgccg ccgccgcata ggccctgcct ccccggccgc
      901 cattttcttc gttggcaaac agcaccacgc ggatcgtatg ccggggttta tacccgatcg
      961 ccttcagcgc ccgcaacacc tcgatcgact gcacgcaacc cgcaccgtca tccatcgccc
     1021 cttccgccgg gtcccaggag tccaggtggc cccccaccgt gatatactga tccggaaact
     1081 ccgatccccg caactccccg atcacattat gcgccagcgt atcgggcaac atctgcgcat
     1141 tcgtacgcag ataaacgctc accccccgtg gcggaccctc cagcagcgcc gccagccggt
     1201 ccgcatcccg aagccccacc gccgccgcgg ggatcttctg cgttgaatcc gcatagatca
     1261 gcgccccggt atgcggatta ttatcgacgg aggaactcat gctccgcacc agcaccgcca
     1321 acgccccata cttcgccgcc cggctggcgc ctgctccccg ataccgcacc gcatcgccat
     1381 aggcctggaa ggtctcgaca aacagcgggt taaatttata gttgtagaaa acgatcttcc
     1441 ctttcagctc gtccttgcgc tgttccagct cgtcgaaatt gtggatcagc accaacggcg
     1501 ccgtgatacc cgcgggtccc gttcccacgg aatttcccag ggcgaggatg tccagccccc
     1561 ccgtcatcac atagtgtgcg ttatacgtcc cggttttagg agcgccggca ttccacctcg
     1621 ccgaatcctt cccaccccgc acccaatgcg gaaccatgca ctcctgcagc cataccttat
     1681 cggccatggc cccggtgagc gccgcctgtc cccatttctc tgctttataa tagccggcgc
     1741 tccccgacaa ccgggcgccc actgtcttcg tcagctcccg caagtcgcca taagccttcc
     1801 catccgtcaa tatcgtatcc gccagccggc ggatcagcgc ggaatcctcc gccgtttgcc
     1861 cagctgtcac tccagccgtc actccggtct tctggccagc cgtcactccg gtcgtctccc
     1921 cggccatttc cttagccgcc tgcccaaacg cccttcctcc cccggccaac cccacaacca
     1981 caaacaaaaa gattagtaaa ctgcgactca acaatttagg gtgcatcgat gtttattttc
     2041 gcactaaggt aaggcgtcgc aggcggacaa aaaaaattcc gcctttgtcg tcgcgggctg
     2101 aaaccgaaaa aataagcccg ctccgccgca ccccggaccc ctcatcaaca aatcgtccgg
     2161 gcgccggcaa attcctttac cttagcaacc gcaaatttcc gcccccggcg gatctaaacg
     2221 ttaaaccatg cgcataggaa tcgtctgtta tcctactttc ggcggcagcg gcgtactcgc
     2281 cactgaactg ggaaaagccc tggccgacaa aggccaccag gttcacttta tcacctacca
     2341 gcaaccggtg cgtctgaacg agttcaacgc gaacatcttt taccacgagg tgcgcgtcac
     2401 cacctacccg ctcttcgact atccccccta cgagacggcc ctgtccagcg ccatggtcga
     2461 cgtcatcaac aaccaccagc tcgacctcct gcatgttcac tacgccatcc cccatgcctc
     2521 cgcggcctat atggccaaga agatcgtcga ggcgcaggga cgctatatcc cggtgatcac
     2581 gaccctgcac ggtacggaca tcaccctcgt gggccgcgac aagaccttcg ccccggtcgt
     2641 cgccttctcc atcaaccaga gcgacgccat cacggccgtc tcgcagaatc tgcgcgatga
     2701 aaccttccgc accttcaaca ttgaaaaaga gatccgcgtc attcataatt ttgtcgacac
     2761 ccgccggttt caccgcaaac cgatcgacgc tttccgccgg gtgatcgccc ccaacggcga
     2821 acgcatcctg atgcatgcct ccaacttccg caagatcaaa cgcgtccagg atatcgtccg
     2881 catcttcgag atcgtccgcc aaaagatccc cagcaagctg ctcttcgtcg gcgacggacc
     2941 cgaacgctcc gaggccgaag ccctctgccg cgagctcgac atcatcgacg acacccgatt
     3001 cgtcggcaag caggagcaga tggaggagat cctcgccgtg gccgacctct tcctgctcac
     3061 ctccgaatat gaaagctttg gcctcgccgc gctcgaagcg atggcggccg gcgtgccggt
     3121 ggtctcgaca aatgcggggg gactcccgga gatcgccatc cagggcgaga ccgggtattt
     3181 ggatgatatc ggggatattc atgggatgag cgagcacgcg atccgcatcc tgcaggacga
     3241 cgagacgctg aaacgcttca aggccaacgc cgccgcccat gcgaagaact tcgacatcca
     3301 tcgcatcgtg ccgcaatacg aggccctcta caaccgattt ttagcgctgg cttagagcgg
     3361 gtccgcgggc cgggaaagcg gggcgcccct taacgggccg ggcagacctt cgcgtcgtcc
     3421 attccctctc attaatggct cccgcaacat catccagcca tcggtctccc gactcc
//
LOCUS       DATGFG010001017         5545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1206240, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001017 DATGFG010000000
VERSION     DATGFG010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206240"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..508)
                     /locus_tag="VKQ52_19290"
     CDS             complement(110..508)
                     /locus_tag="VKQ52_19290"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ89412.1"
                     /translation="MQKITNLLMFDGRAEEAINFYISVFPNSGLLHITRYGPNEAGPE
                     GSVVHASFVLYGQVFTCIDSYTKHAFTFTPSLTLCVNCETEDEVDDIFDKLTKGGKVL
                     MPMGPYPFNKKFGWATDKFGVSWQVSLQPS"
     gene            595..2820
                     /locus_tag="VKQ52_19295"
     CDS             595..2820
                     /locus_tag="VKQ52_19295"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HLZ89413.1"
                     /translation="MNKVLLTASWLAIAVAARAQSNRVITTEEYAHAESMLNYNTEPL
                     IDRATVQPNWLPGGGLFWYRTLTATGSEYVLVDAGKKSRVSYGDREKLAQAISTATGK
                     PFDVSMLAEGRRGRGGRGGGNEVLSPDGKRAAFIRDYNLWVREVPSGEEVQLTTDGIK
                     DFGYATDNAGWKSSEGPILRWSADGKKIATFRDDQRHVSDMYLVTTNVGKPVLHAWKY
                     PLPGDSVIPMIQRVIIDVDARKLVVLNVAPDPHRATLSDDIASSGAFDDVDWTDDDAQ
                     LAFVSTSRDHKQEKFRIADAATGAVREVFGEDVPTQFESGRGAINWRYLARSNEIIWY
                     SERDNWGHLYLYDAATGKVKNQITKGEWLVARLLRVDEKKRELYFLGAGREPGCPYFN
                     YLYKTGFDGKKLQLLTPESGNHTVNWSANGEYFVDSYSRPDAAPVSVLRNAEGKLITP
                     LEQADISRLVAAGWKPPTPITVKANDGKTDLYGLLFTPTHLDSTKKYPVVDYIYPGPQ
                     GGSVGNWNFVASRNDNQALAELGFIVVEIEGTSNPYRSKGFHDMSYGNMAENTLPDQV
                     TGITQLAKRYSFIDIDRVGIWGHSGGGFATAGAMFRYPDFFKVGISESGNHDNRNYED
                     DWGERYHGLLVKNADGTDNYTAQANQGLAKNLKGKLMLAHGMMDNNVPPYNTLLVVEA
                     LEKANKDYDLVIFPNSPHGYGPNGPYMMRRRWDYFVKNLMGAEPPHEYQMKPRADPRN
                     M"
     gene            2996..4261
                     /locus_tag="VKQ52_19300"
     CDS             2996..4261
                     /locus_tag="VKQ52_19300"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLZ89414.1"
                     /translation="MEELLGMDKEIGAMVAGRKRRRLLVMICAGAVLLAGATGLVRRT
                     FRSTIKRSQITIATVERGDVDNTITATGEVLPEFEEILTSPINATIRRALTDAGDPVT
                     AGQSVLELDKGASQLDYENLNFQLESKRNSIHKLKLELDQSFFDLRSNNDIKQLKINS
                     LQAAVENAKRLLQAGGGTKEDVEQAELNLQVAQLEKQQLENEIRSKQQTMQADMRESE
                     IAARVQENDLLVLKRKLELANLVASRPGIVTWINKNIGATVKEGESLARIADLTGFKV
                     SGSVSDNYINELHRGMPVIVRVNDVSLAGRMVNIYPAVQNGIVTFDVQLNERNDPHLR
                     PNMKVDVHLVTASRSKVLRVANGSAFRGPGDLDVFVVDKGEAVRRRVHVGVSNFDWVE
                     LKDNVAVGDKIIVTDLSDYKNTDVIQIVQ"
     gene            4239..>5545
                     /locus_tag="VKQ52_19305"
     CDS             4239..>5545
                     /locus_tag="VKQ52_19305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ89415.1"
                     /translation="MLYRLFNRCGRRGWRAAFCGRGNCFGGWIVAGLLLAASKRGFAE
                     DTLRISLQQVVELAKERSIAARQAATVKETKYWQWRVYRSNYQPQLALNGTFPAYSKT
                     FTQVLQPDGTILFQPIYNNNSNLNLSFSQTIAATGGTIFGTTQLQRFDDFDRHNTLYN
                     AVPFGIGYSQPLWQFNQLKWDNRIEPLKYDESRQAFVESMEQIAVTVTGYFFDLLLAQ
                     VNLQIAETNLGNTRSILRIADEKLKLGKISKNELLQLQLERLKAMKAAGIARRDVEIA
                     TLTLRSNTGLPGAGAVLLELPPAGIAMTVSAAKVLEEAYANRSDAIAFVRRLREASRD
                     IALARGQSGLNASLTARLGYSNSATTFSKVYHDPQDQQLLEVDFSIPILDWGRSKSRI
                     RTAQANEQFTRYSVEQDKQVFTQQIVTQVTLFEMMRDQVSLTA"
BASE COUNT         1179 a         1242 c         1960 g         1164 t
ORIGIN      
        1 aggtgttcgt gggtgtggaa gggttggcgg tagtcgtgtt ccagcatcca gaagcactgg
       61 atgtagggtt ggaggtgtgg tggcggcgga tattcgtggt agaacatggt caggaaggtt
      121 gaaggctgac ctgccaggag acgccgaatt tgtcggtggc ccagccgaat tttttgttga
      181 agggataggg gcccatgggc atcaggacct tgccgccctt tgtgagtttg tcgaatatgt
      241 cgtcgacctc gtcctcggtt tcgcagttga cgcagagggt gagggagggg gtgaaggtga
      301 aggcgtgttt tgtgtagctg tcgatgcagg tgaagacctg gccgtagagg acgaagctgg
      361 catggacgac ggagccttcg gggccggctt cgttggggcc gtaccgggtg atgtggagga
      421 gaccggaatt tgggaagacc gagatataaa aattgatcgc ctcttcggcg cggccgtcga
      481 acatgaggag gttggtgatc ttttgcacgg tcagtcgctt ttcgagtatg aatttaagac
      541 attttcgaat gggggcgaag gggaaaatga tgtatttact gtatattcgg cggtatgaat
      601 aaagttttat tgacggcctc ctggcttgca atagccgtgg ccgcccgggc ccaatccaac
      661 cgtgtcatta ccactgaaga gtatgcgcat gcagaaagta tgctgaatta taataccgag
      721 ccgctgatcg accgggcgac ggtgcagccg aactggctgc cgggcggcgg tttgttctgg
      781 taccggacgc tgacggcaac ggggagtgag tatgtactgg tggatgcggg gaagaagagc
      841 agggtgtctt acggcgatcg cgagaaactg gcgcaggcca tttcgactgc gactgggaag
      901 cccttcgacg tgtcgatgct ggccgagggc aggcggggcc ggggcggcag ggggggtggc
      961 aatgaagtat tgtcgccgga tggtaagcgc gcagccttta tccgcgatta taatttatgg
     1021 gtgcgggagg tgccttcggg cgaggaggtg cagcttacca ccgatgggat aaaagatttt
     1081 ggttatgcga ccgataatgc cggatggaag agtagcgagg ggccgatcct gcgctggagc
     1141 gccgacggca aaaagatcgc gacgttcagg gacgatcaac ggcatgtcag cgatatgtat
     1201 ctcgtgacga cgaacgtcgg caagcccgtg ctgcacgcat ggaaataccc gttgccggga
     1261 gattcggtga tcccgatgat ccagcgggtg atcatcgacg tggacgcccg gaagctcgtg
     1321 gtgctgaatg tagcgccgga cccgcaccgc gctacgctga gtgatgatat cgccagcagc
     1381 ggggcctttg acgatgtcga ctggaccgat gacgatgcgc agttagcctt tgtctccacg
     1441 tcccgggatc ataagcagga gaagtttcgc atcgcggatg cggcgacggg cgctgttcgc
     1501 gaagtattcg gggaagatgt gcccacgcag ttcgagtcgg gccggggagc gatcaattgg
     1561 cggtacctgg ccaggtcaaa tgagatcatc tggtactcgg agcgggataa ctgggggcat
     1621 ctttacctgt atgatgcggc gacgggcaag gtgaagaacc agatcacgaa gggcgagtgg
     1681 ctggtcgcgc ggctgctgcg cgtggatgag aagaagcggg agctgtattt ccttggtgcg
     1741 ggcagggagc cgggttgtcc ttatttcaat tatctataca agaccggttt cgatggtaag
     1801 aagttgcagt tgctgacccc cgaatcgggc aatcatacgg tgaactggtc ggccaacggc
     1861 gaatattttg tcgatagtta ttcccggccg gatgcggcgc cggtgagtgt gttgcggaat
     1921 gccgagggga agctgatcac accgctggaa caggccgata tctcccggct ggtggcagcg
     1981 ggctggaaac cgccgacgcc gataacggtg aaggcgaatg acgggaagac ggacctgtac
     2041 ggattgttgt ttacgccgac ccatctcgac agtacgaaga agtacccggt agtggattat
     2101 atctatcccg gtccgcaagg aggcagtgtc ggcaactgga attttgtcgc gtcgcggaac
     2161 gataaccagg cgcttgcgga gcttgggttt atcgtggtgg agatcgaggg gacgagtaac
     2221 ccgtaccgca gcaagggttt tcatgatatg agttatggga atatggcgga gaatacgttg
     2281 ccggaccagg tgacggggat cacgcagttg gcgaagcggt attcgttcat cgatattgac
     2341 cgtgtgggga tctggggtca ttccggcggc gggtttgcga cggcgggtgc gatgttccgg
     2401 tatcccgatt tcttcaaggt ggggatctcg gaatcgggca atcatgataa ccgcaactat
     2461 gaggatgact ggggcgagcg gtatcacggg ttgctggtga agaatgcgga tggaacggat
     2521 aattataccg cccaggccaa ccagggcctt gcgaagaatt taaaggggaa gctgatgctg
     2581 gcgcatggga tgatggataa taatgtgccg ccctataaca ccctgctggt ggtggaggcg
     2641 ttggagaagg ccaataagga ttatgacctt gtgatcttcc ccaatagtcc gcatggatat
     2701 gggccgaacg ggccgtacat gatgcgccgg cggtgggatt attttgtaaa gaatttgatg
     2761 ggggcggagc cgccgcatga ataccagatg aaaccaaggg ccgacccgag gaatatgtga
     2821 taggcgtgga ggaccagggg gcgacgccgg cgagacggcg gtgggtgttg tccgatagta
     2881 aaacgagttg tccggaatcg gacttttcag acggctggag ttatcgtaaa cagatgattt
     2941 ccagccgttt ttctttggca cccggcttgt atccttttcc tgcaaatact acctgatgga
     3001 ggagcttttg ggaatggaca aggagatcgg cgcgatggtg gccggccgta agcggcgcag
     3061 gctgctggtg atgatctgtg cgggggccgt gctgctggcg ggggcgacgg ggctggtgcg
     3121 gcggaccttc cggagtacga tcaagcggtc gcagatcacg atcgcgacgg tggagagggg
     3181 cgatgtggac aatacgatca cggctaccgg ggaagtgctc cccgagtttg aggagatcct
     3241 gacgagcccg atcaatgcca ctatccggag ggcgttgacg gacgccggtg acccggtaac
     3301 ggcggggcag tcggtgctcg aactggacaa gggggcgtcc caactggact atgagaacct
     3361 gaacttccag ctcgagtcca agcgcaacag tatccataag ctgaaactgg agctggacca
     3421 gagctttttc gatttgcgat cgaataacga tatcaagcaa cttaagatca acagtctgca
     3481 ggccgcggtg gagaatgcga agcggttgtt gcaggcgggg ggcgggacga aggaggatgt
     3541 ggagcaagcc gagctgaacc tgcaggtcgc gcagctggag aaacagcagt tggagaatga
     3601 gatcaggagc aagcagcaga cgatgcaggc cgatatgcgg gagtcggaga tcgcggcgcg
     3661 ggtgcaggag aacgacctgc tcgtgctgaa acgcaaactg gagcttgcca acctggttgc
     3721 cagccggccg gggatcgtga cctggatcaa caagaatatc ggcgccacgg tgaaggaggg
     3781 agagtcgctg gcgcggatcg ctgatcttac cggcttcaag gtgagcggca gtgtttccga
     3841 caattatatc aatgagcttc accgggggat gccggtgatc gtgcgggtga atgatgtgtc
     3901 gctggcaggg cggatggtca atatctaccc ggcggtgcag aatgggatcg tcaccttcga
     3961 tgtgcagctg aatgagcgga acgacccgca tttacggccc aatatgaagg tggatgttca
     4021 cctggtgacc gcctcgcgga gcaaagtgtt gcgggtggcg aacgggtcgg cgttccgcgg
     4081 gccgggtgac ctggatgttt ttgtcgtgga caagggggag gcggtgcggc ggcgtgtgca
     4141 tgtcggggtg agtaatttcg attgggtgga gttgaaggat aatgtggcgg tgggggacaa
     4201 aattattgtt acggatctaa gcgattataa gaatacagat gttatacaga ttgttcaata
     4261 gatgcggccg ccgcgggtgg agggctgctt tctgtggccg ggggaattgc tttggcgggt
     4321 ggattgttgc cgggctgttg ctggcggctt cgaagagggg gtttgcggaa gatacgttac
     4381 ggatctcgct gcagcaggtg gtggagcttg cgaaggagcg gtccattgcc gcgaggcagg
     4441 cggcaacggt gaaggagacg aagtactggc agtggcgggt ctaccggtcg aattatcagc
     4501 cgcagctggc gctgaacggc acttttcctg cctacagcaa gacctttacg caggtgttgc
     4561 agccggacgg tacgatcctt tttcagccta tttacaataa caattccaac ctcaaccttt
     4621 cctttagcca gacgatcgcg gccaccggcg ggacgatctt cgggactacg cagttgcagc
     4681 gcttcgatga ttttgaccgg cacaatacgt tgtataatgc cgtgcccttt ggtatcgggt
     4741 atagccagcc gctgtggcag ttcaatcagc tgaaatggga caaccggatc gaaccgttga
     4801 aatatgacga gagccggcag gcctttgtcg agtcgatgga gcagatagcg gtgacggtga
     4861 cggggtattt tttcgatctg ttgctggcgc aagtgaattt acagatcgcg gagaccaatc
     4921 ttggcaatac ccggtcgatc ctgcggatcg ctgatgagaa actgaagctg gggaagatct
     4981 cgaagaatga attgttgcag ttgcagctgg aacggttgaa ggcgatgaag gcggcgggga
     5041 tcgcgcggcg ggatgtggag atcgcgacgc tgaccttgcg gagtaatacc gggttgccgg
     5101 gtgcgggcgc cgtgttgctg gagctgccgc cggcggggat cgcgatgacg gtgagcgccg
     5161 ccaaggtact cgaggaggcc tatgccaacc ggagtgatgc gatcgccttt gttcgccggc
     5221 tccgtgaggc cagccgggat attgccctgg cgagggggca gagcgggttg aatgcctcgc
     5281 tgacggcgcg gctgggctat tcgaacagcg ccactacttt ttccaaggtg tatcatgatc
     5341 cgcaggatca gcagctgttg gaagtggatt tttcgatccc gatcctggac tggggccgga
     5401 gcaagtcgcg gatccggacg gcgcaggcga atgagcagtt cacgcggtac tcggtggagc
     5461 aggacaagca ggtgttcacg cagcagatcg tgacccaggt gacgcttttc gagatgatgc
     5521 gggaccaggt gtcgcttacc gcgga
//
LOCUS       DATGFG010001018         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1151397, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001018 DATGFG010000000
VERSION     DATGFG010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151397"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VKQ52_19310"
     CDS             <1..773
                     /locus_tag="VKQ52_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89416.1"
                     /translation="ACTDVLIRDCTIRSSANAIKFGTVSRGGFRHIRILHNNVYDTYR
                     SALALEAVDGAAIRDVVADSLTAVNTGNALFIRVGARSGSRQSSLADVSIRHLSVEIA
                     AAKADSGYPYEGPVEDQPRNISPIVLTSLPGALLQDIRLEDVTVRYPGGADPFIAHGN
                     PRGGNPFIASRAPADSVPELPANYPEFSMFKELPAWGLYIRHARGIQVKNLRLYCAAK
                     DYRTAIVLDDVAGDTWEATVVKEPGNKKDRLHIIPFSH"
     gene            complement(817..1569)
                     /locus_tag="VKQ52_19315"
     CDS             complement(817..1569)
                     /locus_tag="VKQ52_19315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ89417.1"
                     /translation="MDRISAIIIDDEKKSRMSLLQKLQSYCPTVEIVGEAANGPAGKE
                     LIESLKPDLVFLDIVMPRMNGFEMIASLSHRNFEIIFTTAYSQYAIQAIRYSAFDYLL
                     KPVDIEDLRNAIQKIGLKIAASDKEERLMVLLQNLSNEKERSKRIVIPTAEGLLFISV
                     DDIIHLEASSNYTFVYFLHEPRLVACRTLKEFEEMLPDSIFFRIHHSHIININAIKKI
                     TRDEGNRVELTNGNVVDISRRRKDEFLKMLGQ"
     gene            complement(1575..>2413)
                     /locus_tag="VKQ52_19320"
     CDS             complement(1575..>2413)
                     /locus_tag="VKQ52_19320"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ89418.1"
                     /translation="PGEYTFLVKTKKQFGDWGPVNDSLSISVAPAFWQTALFRLLCLV
                     LVAACTWWLLRRRIGRVRQESELKRQIAETEMRALRAQMNPHFIFNCISGIDSLIQCN
                     DKYNATTYLNKFAKLIRNVLESSKNETIPLWKDIETLKLYVELEAMRNDNEFHFDFDI
                     DPILLRGDYHVPSLIVQPYIENAIHHGIRNRQDDQGRLEITIRAEESFLIYRITDNGV
                     GRKAAAEWSKSMHQSYGLAVSQERINLFNRRKTKSVIITDLYDNNDEPLGTQVIVKLS
                     LN"
BASE COUNT          523 a          633 c          629 g          628 t
ORIGIN      
        1 cggcctgtac cgacgtgctg atccgggact gtacgatcag gtccagcgcc aatgcgataa
       61 aattcggaac agtatcgcgc ggtgggttcc ggcatatccg catcttgcac aacaacgtgt
      121 acgataccta ccggtcggcg cttgcgctgg aggcggtcga tggggcggcg atccgggacg
      181 tggtcgcgga tagtctgacg gcggtgaaca cgggcaatgc cctcttcatc cgcgtcgggg
      241 cgaggagcgg ttctcgccaa agtagtctgg cggatgtttc catccggcat ctgtccgtcg
      301 aaatagccgc cgccaaggcg gattccggct atccctatga ggggccggtc gaagaccagc
      361 caagaaatat ctctcccatc gtgctgacca gccttcccgg agcgctcctg caggatattc
      421 gattggaaga cgtgaccgtt cgctatcccg gcggcgcgga tccgttcatc gcgcatggca
      481 accccagggg cgggaatccg ttcatcgcgt cgcgcgcccc ggcggacagc gtgccggaat
      541 tacccgccaa ctatcccgaa ttctcgatgt tcaaagaatt gcctgcatgg gggctgtata
      601 tccggcatgc acgcgggatc caggtcaaaa acctccgtct ttactgtgcg gcgaaagatt
      661 accgcacggc gattgtactg gatgatgtgg caggggacac ctgggaggca acggtggtca
      721 aagaacccgg gaacaagaag gaccggctac acatcattcc tttttctcac taacctcata
      781 atcctcactc cgttctcagg ctctccaccg gattcgctat tggcccagca tcttcagaaa
      841 ctcgtccttc cttctccggg aaatgtcgac cacattaccg ttggtaagct caacccggtt
      901 gccttcgtcc cttgtgatct ttttgatggc atttatattg atgatatgcg aatgatgtat
      961 tctgaaaaag atggaatcgg gcagcatttc ttcaaattcc tttaatgtcc tgcaggcgac
     1021 aagcctgggc tcgtgcagaa aatagacgaa ggtatagtta ctgctggctt ccaggtggat
     1081 gatatcatcg acagagatga acaggaggcc ttccgcggta gggatcacga tgcgtttgct
     1141 gcgttccttt tcattggaaa ggttttgcag cagcaccatc agtctttctt ccttgtcgga
     1201 ggccgcgatc ttaaggccta ttttttgtat ggcatttctc aggtcttcaa tatccacggg
     1261 cttcaataaa tagtcgaatg cgctgtagcg gatggcctgg atggcatatt gactatatgc
     1321 cgtggtgaag atgatctcga aattcctgtg tgaaagggat gcgatcattt cgaagccgtt
     1381 catccggggc attacaatgt ccaggaagac caggtccggc ttgagcgatt cgataagctc
     1441 ctttccggcc ggcccgtttg cggcctcgcc gacgatttcc accgtcgggc aatagctctg
     1501 taatttttgc agcagggaca tccggctttt tttctcatca tcgattatta tggcggatat
     1561 cctgtccatc ggggttagtt tagtgaaagc tttacgatta cttgtgttcc aaggggctca
     1621 tcgttattat cgtacaggtc cgtaatgatg acactttttg ttttcctccg gttaaagagg
     1681 ttgatgcgtt cctggctcac cgcaaggcca tacgattgat gcatggattt actccactcg
     1741 gcggccgctt tcctgcccac tccgttgtca gtgatccggt atatcagaaa agactcttca
     1801 gcccggatcg ttatttccag ccttccctga tcatcctgcc ggttgcggat cccgtgatgt
     1861 atggcgtttt caatataggg ctgaacgatc agggaaggaa catggtaatc cccccgcaat
     1921 aagattggat cgatatcgaa atcaaaatgg aattcgttgt cattacgcat cgcctcgagc
     1981 tccacatata gctttagggt ttcaatgtct ttccacaaag gaatggtttc atttttggaa
     2041 ctctccagta cattccgtat cagtttggcg aatttattca ggtaggtcgt ggcattgtat
     2101 ttatcattgc actggataag gctgtctatc ccgctgatgc aattgaaaat aaagtgcggg
     2161 ttcatttgcg ctcgtaatgc ccgcatttcc gtctcggcga tctgccgctt cagttctgat
     2221 tcctggcgca cacggccgat ccgccgtctc aacagccacc aggtgcatgc ggctaccaat
     2281 accaggcata acaatctgaa cagtgccgtt tgccagaacg caggcgcaac ggagatggaa
     2341 agactgtcat tcaccgggcc ccaatcccca aactgctttt tcgtttttac aaggaaggta
     2401 tattctcccg ggg
//
LOCUS       DATGFG010001019         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1656340, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001019 DATGFG010000000
VERSION     DATGFG010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656340"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VKQ52_19325"
     CDS             complement(<1..529)
                     /locus_tag="VKQ52_19325"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HLZ89419.1"
                     /translation="MEEKQQHFRKIDFLRGIAILTVYQAHFIWYYFPGYAGMVGVDSG
                     HPASKVLLLNLLPRTIGWGGVTIFILVSGFLLHLGYLRDPERFTLRGFYSRRFWRVYP
                     PYLLVLLVFSLFLEKDLFGSRFGWLTFFLHVFSLHNLFTRTYFSVNPTFWCLALEVQL
                     YVLYAVFLYGRKRWGV"
     gene            789..1289
                     /locus_tag="VKQ52_19330"
     CDS             789..1289
                     /locus_tag="VKQ52_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89420.1"
                     /translation="MKQLFSLLLFLGLASVGMGQLKAKVKCPDFYVDVLDGTVNGLKP
                     SYTQLEIKEKFPCFTSAQDESQEAKCGGGIFFKDKDIFFYTKRKYVEVGPKFIGKTSI
                     PLLGTKRGSLFTKLGNPKIKDDLWDAYDTQYGCLVLHYDVAGAAGKVKYFQFSTLGTD
                     AINLCE"
     gene            complement(1273..1809)
                     /gene="idi"
                     /locus_tag="VKQ52_19335"
     CDS             complement(1273..1809)
                     /gene="idi"
                     /locus_tag="VKQ52_19335"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HLZ89421.1"
                     /translation="MEREEVILVNQEDTPLGTMEKMEAHRKALLHRAFSVFIFNSRGE
                     MLLQRRAPGKYHSPGLWTNACCSHPRPGEETRAAACRRLREELGFTTELEKLFDFTYR
                     SEFDNGLTEFEFDHVFVGHYDKEDIRPNAAEVSDYGYRSLEEIKEGLVAHPEFFTSWF
                     HLAFPLVMTKMAGAIRKG"
     gene            complement(1822..2322)
                     /locus_tag="VKQ52_19340"
     CDS             complement(1822..2322)
                     /locus_tag="VKQ52_19340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLZ89422.1"
                     /translation="MATVEFNQLLVNNAEFLKPFAITLTRDSEAAKDLFQETLFRALA
                     NKDKYNVGTNIKAWLYTIMRNIFINNYRRKAKQNTIFDSTPNEFLLNSAQMAVPGSVE
                     SNLRVKDIQAAIHKLPEIFRNPFLLYFDGFKYHEIAEMLQEPLGTIKSRIHFARKLLK
                     NQIQRF"
     gene            complement(2618..3493)
                     /locus_tag="VKQ52_19345"
     CDS             complement(2618..3493)
                     /locus_tag="VKQ52_19345"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLZ89423.1"
                     /translation="MNVFTIKDLENLSGIKAHTIRIWEQRYNFLKPCRTETNIRYYCN
                     QELKTVLNISLLNRYGYKISHINKMSEQELRSKIISLTDRDAQQERLVNELIAHMIDL
                     DIEQFETVLDDFILARGIDKAIPQVIFPFLHRIGILWLTNHIHPAQEHLVTQVIRRKI
                     LVGIDGCLPCKRRGKLVLLFLPEGEYHELGLLYVYYLLKTNGVRVLYLGADLPLKDVE
                     FVCRAKNPDYLYTHLTGIAGSFNFEKYLSLVTERIAIPLVISGQLARTKLRKTPPGVN
                     FRRSIPEVLEFIATL"
     gene            3586..4242
                     /locus_tag="VKQ52_19350"
     CDS             3586..4242
                     /locus_tag="VKQ52_19350"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLZ89424.1"
                     /translation="MSISDISLKRPITSYSKVVKFLSDLKRGRKLFIRKKRIKDKVLL
                     NVGCGPLSREEFINLDYEWTPTIDIVWDFTKKPIPLPSESLEGIYTEHCLEHITLDAC
                     LGTLKDFHRLLKPGGNVRIIVPDAQIYLDLYQARKTDPSIVLPYGEKEATAAISINRI
                     MRLHGHLFIYDAETMGYLLEKAGFRNIKKESFSHGRDKRLLIDLEARKVESLYMEATK
                     "
BASE COUNT         1126 a         1130 c         1148 g         1019 t
ORIGIN      
        1 ttacccccca ccgcttccgg ccatacaaaa aaaccgcata cagcacatac agctgcacct
       61 cgagggcgag acaccagaag gtgggattca cggaaaaata ggtcctggta aacaggttat
      121 gcagcgagaa cacatgcagg aagaaggtca gccatccgaa gcggctgcca aacaggtctt
      181 tctccaaaaa aagactaaag acaagcagga ccagcaggta gggcggatag acccgccaga
      241 aacgccggct gtaaaaccca cggagagtaa accgctccgg atcacgcaga taacccagat
      301 gtaaaaggaa tccgctgaca agtatgaaga tcgtcacccc accccagccg atcgtccggg
      361 gaaggaggtt cagcaacagc accttcgatg ccggatgccc gctgtccaca ccgaccatcc
      421 cggcatagcc gggaaaataa taccagatga agtgggcctg gtaaacggta aggatggcga
      481 tgccccgcaa aaaatcgatc ttccggaagt gctgctgttt ctcctccata ggcggcatgc
      541 ggtattcggt gcgtggtaag ataagcaatt tcggcccgga ctccatcccg gcgcggggaa
      601 tttttcccgg ggagagaaaa ttttcggaaa gagcaatcta gcaaggattt tgctgtagga
      661 attgcggcaa aaaatgaacg aaaaataatt ttttgccgca acatcaataa tatctgtaca
      721 cttcggctga agcctcgtgt acggaccgga ttccatccgg tcgaatccgt ctaaaatcaa
      781 tatagcttat gaagcaactc ttttccctgc tgttgttcct tggccttgcg tccgttggta
      841 tgggacaact aaaagccaag gtaaaatgcc ccgactttta tgtagatgtg ctcgacggca
      901 cggtcaatgg tctcaagccg agttataccc aactggagat caaagaaaaa tttccctgtt
      961 tcaccagcgc ccaggacgag tcccaggaag ccaaatgcgg tggcggcatc ttcttcaagg
     1021 ataaagatat tttcttttat acgaagagga aatatgtcga ggtcggcccg aaattcatcg
     1081 gcaagaccag catcccgctg ctgggcacga aacgcggttc cctgttcacc aaactcggta
     1141 acccgaagat caaggacgat ctctgggatg cttacgacac gcaatacgga tgtctcgtcc
     1201 tgcactatga tgtggccgga gcggccggga aggtaaagta tttccagttc agcacactgg
     1261 gcaccgacgc catcaacctt tgcgaatagc gcccgccatc ttcgtcatta ctaacgggaa
     1321 ggccaggtgg aaccagctgg taaaaaattc cggatgggcc acaaggcctt cttttatttc
     1381 ttccagggaa cgatacccgt aatcactgac ctccgccgcg ttgggccgga tatcctcctt
     1441 gtcatagtga cccacaaaga catggtcgaa ctcgaactcc gtaagaccgt tgtcaaattc
     1501 cgaacgataa gtaaagtcga agagtttttc cagctcggtg gtaaagccca gctcctcccg
     1561 gagcctgcgg caggccgccg cacgcgtttc ctcccccggg cgcggatgac tgcaacaggc
     1621 gtttgtccac aaaccgggag aatgatactt gcccggcgcg cggcgctgca ataacatctc
     1681 acccctggaa ttgaagataa aaacgctaaa agccctgtgt aaaagggcct tgcggtgtgc
     1741 ttccattttc tccatcgttc ccagcggagt atcctcctgg ttcaccagga tcacttcttc
     1801 ccgttccata gcccaagata atcaaaatcg ctggatctgg ttcttcagca gcttgcgggc
     1861 aaaatggatg cgactcttaa tggtacccag gggctcctgt aacatctcgg ctatctcgtg
     1921 atacttgaag ccatcgaaat acaacaggaa aggattgcgg aatatctccg gcagcttatg
     1981 gatcgccgcc tggatatcct tcacccggag attgctttcg acagaacccg gcaccgccat
     2041 ttgtgcggag ttgagcagga actcattcgg cgtactgtca aaaatggtat tttgtttggc
     2101 tttacggcgg taattgttga taaagatatt ccgcatgatc gtgtaaagcc atgctttgat
     2161 attagtacct acattgtatt tatccttatt ggctaaggct ctgaataagg tttcctggaa
     2221 aaggtctttt gctgcttcag aatcacgggt aagtgtgata gcgaacggct ttaagaactc
     2281 cgcattgttg accaaaagct ggttgaattc tactgtagcc atgagttaaa gttgtttaat
     2341 gatcttctat tcaaatttaa acattaaagg acaacgtctt tggattatgt gacctacggt
     2401 ttcgttaaaa aacaataggt ggataatcaa tcggttgtac tttttcgtct gttgattcgg
     2461 ttgccatgct gttattcttt cctttttact atgcaaaaaa ggggcctgta ggttcggggt
     2521 gccagaaaga cagcgaaagg tacaagagaa ttggcagtct ggacaaaaaa ttggcagaaa
     2581 ctcggtgggg aatcggcaag cggagcaggc caggttgcta caacgtagcg ataaactcca
     2641 aaacttcggg gatcgaccgg cgaaaattca cgccgggagg cgtctttcgc agtttggtcc
     2701 tggccagctg gccggagatg acaaggggga tggcaatacg ttcggtcacc agggagaggt
     2761 atttctcgaa gttgaagctc ccggcgatgc ccgtcaggtg ggtataaaga tagtccgggt
     2821 tctttgcccg gcagacgaac tcgacatcct ttaacggcag gtcggcgccc aggtataata
     2881 ccctgacacc gtttgtttta agaagataat acacatacag gagccccagc tcatggtatt
     2941 caccctccgg gaggaagagc aggaccagct tcccccttcg cttgcagggc aggcacccgt
     3001 cgatgccgac gagtattttc cggcggatga cctgggtaac caggtgttcc tgggcgggat
     3061 ggatatgatt ggtcagccaa aggatcccga tccggtggag aaaggggaaa atgacctggg
     3121 ggatggcctt gtcgatcccc ctggcgagga taaagtcgtc gagcaccgtt tcaaactgct
     3181 cgatatccag gtcgatcata tgggcaataa gctcgttgac cagccgctcc tgctgcgcat
     3241 ccctgtccgt cagggagatg atcttgctcc gcagctcctg ctcgctcatt ttgttgatat
     3301 gggagatctt gtacccatac cggttgagca gggagatatt cagcaccgtc tttaattcct
     3361 ggttgcaata gtaccggata ttggtttctg tcctgcacgg tttcaggaag ttatagcgct
     3421 gctcccagat ccggatggta tgggccttga tccctgaaag gttctccagg tcttttatgg
     3481 taaagacgtt cactatatgg tttacaaccc cccggtcctt tttgttttat attttgctat
     3541 tcaaaaagtt agccgttatt tagcatcgct gtaaagaacc cacccatgtc aatatccgat
     3601 atcagcctga aaaggcccat tacttcctat tcgaaagtgg ttaaattcct ttccgatctc
     3661 aaaaggggca ggaagctctt catcaggaaa aagcgtatta aggacaaagt attgctcaac
     3721 gtaggctgcg gccctctctc ccgcgaggag ttcatcaatc tcgactacga atggacgccg
     3781 acgatcgata tcgtctggga tttcacgaaa aagccgatcc ccctgccttc ggagtccctg
     3841 gagggcattt ataccgagca ttgcctggag catatcaccc tcgacgcctg cctcggcaca
     3901 ttaaaggatt tccaccgcct gctaaagccc ggcggcaacg tgcggatcat cgtcccggac
     3961 gcccagatct acctcgatct ctaccaggcg cggaagacgg atccttccat cgtcctgccc
     4021 tatggcgaaa aggaagcgac ggccgccatc agcatcaacc gcatcatgcg gctgcacggt
     4081 catctcttca tctatgacgc cgaaacaatg ggttacctgc tggaaaaagc cgggttcagg
     4141 aatataaaaa aggagagttt cagccacggc cgggataagc ggctgctcat cgacctggag
     4201 gcaagaaagg tggagagttt gtatatggag gcgacgaagt aaacgggtcc accgggggac
     4261 cgccggcccc gcatgcaagg gtggggctac acgggctggg gctacaactg cgggttgagg
     4321 agcttccccg catccagcag ggcattcacc ttatggaata tttttggcga atccagcaat
     4381 tgcaggtgct gggcgcgatg gagatcggtc cccagcaggt cca
//
LOCUS       DATGFG010001020         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_968558, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001020 DATGFG010000000
VERSION     DATGFG010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968558"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VKQ52_19355"
     CDS             <1..210
                     /locus_tag="VKQ52_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89425.1"
                     /translation="TSDQLPINLLTSLKKEYGDYWISDLFEIASDDQTNYYVTLETAD
                     KKIVLKSQGTEFWNEYKKEKKTTTL"
     gene            363..1337
                     /locus_tag="VKQ52_19360"
     CDS             363..1337
                     /locus_tag="VKQ52_19360"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HLZ89426.1"
                     /translation="MSKGPISQFIEHHYRHFNAAALVDAAKGYETHLLEGGKMMITLA
                     GAMSTAELGISLAEMIRQGKVDIISCTGANLEEDVMNLVAHSHYKRVPNYRDLTPQDE
                     WNLLENHFNRVTDTCIPEEEAFRRLQKHLVKVWRDAEAKGERYFPHEFLYKVVLSGDL
                     EQYYEIDPKNSWIVAAAKKNIPIVVPGWEDSTTGNIFASYVIKNELKASTVKSGIEYM
                     TWLAGWYRENSGGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSFGSYSGAVPNEKITWGKLDIHTPKFIVESDATIVAPLIFAWVLGW"
     gene            1362..1877
                     /locus_tag="VKQ52_19365"
     CDS             1362..1877
                     /locus_tag="VKQ52_19365"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89427.1"
                     /translation="MNANSEELYLEAEADIKNNNYAEAFKKYESILYEEPDSAPAHNS
                     MGWLYKTQFDDYAKAENHFLTAIKCDPLYPHSYFHIAALLMDMERFDELRQHLDRCLR
                     VRTIDKSWVYGRYALSEEQRRNYEKAVFYFEKAILVSQNDEKIKDYRQDIERCQMKTD
                     IVTRHKEQEEK"
     gene            complement(1893..2750)
                     /gene="tatC"
                     /locus_tag="VKQ52_19370"
     CDS             complement(1893..2750)
                     /gene="tatC"
                     /locus_tag="VKQ52_19370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HLZ89428.1"
                     /translation="MALNLFNRNQGEDEMSFVDHLEALRWHIVRSAFAIVIIAIIIFV
                     KMNWIFDKIIRGPIRKDFVSYTGLCSLGRKLHMGDALCLDSVAGLQLQTTQFSSQFIS
                     SINIAIVGGFIVAFPYVFWELWRFVKPALSPKELKSSRGAIFWVSFFFLLGVAFGYFL
                     LAPFTFSFLFNYTLGGAGAIKTIPTLDDYVENLVDIIIGSGLAFQLPLVSYVLTAIGL
                     ITPKFLRTYRKYAYVAILFIAAIITPSPDWMSQTIVALPLILLYELSIRVSVRVSKRQ
                     EDRDKEEWS"
BASE COUNT          798 a          714 c          812 g          672 t
ORIGIN      
        1 acatccgatc agcttccgat caacctgctt acttcgctca agaaggagta tggcgactat
       61 tggatctccg atcttttcga gatcgcatct gatgatcaga cgaactatta tgtgacgctc
      121 gagaccgccg acaaaaagat cgtgctgaaa tcccagggca ccgagttctg gaatgaatac
      181 aagaaagaga aaaagacaac cacattataa accagttgta gatttttcga ggttagtttt
      241 ttagttggca aatggccgcc ctttcgggtg gccattttgt tggggcctgt accgacgaaa
      301 cggcggcccg ggtttgcggg aaaatatata tttttgcgcc tcaatcttca aacgaaccta
      361 ccatgagcaa gggtccaatc tcgcaattca ttgagcatca ctacaggcat ttcaacgcag
      421 ccgcacttgt ggacgcagcc aaagggtatg aaacgcattt actggaaggc gggaagatga
      481 tgatcactct cgcgggcgcg atgagtacgg ccgaactcgg catctccctg gccgaaatga
      541 tccgccaggg gaaggtggat atcatcagtt gcacgggcgc caacctcgaa gaagatgtga
      601 tgaacctggt ggcgcacagc cattataaac gggtacccaa ttaccgggac ctgaccccgc
      661 aggacgaatg gaatttactg gagaatcatt tcaaccgggt tacggatacc tgtatccccg
      721 aagaagaggc cttccgtcgg ttgcagaaac atctcgtcaa ggtttggcgg gatgcagaag
      781 caaagggcga acggtatttc cctcatgaat tcctttataa ggtagtcctc agcggcgacc
      841 tggagcaata ttatgagatc gatccgaaga acagctggat cgtagccgcg gcgaaaaaga
      901 acatccccat tgtcgttccg ggttgggaag acagcacgac ggggaatatt tttgccagct
      961 atgtgattaa gaatgagctg aaagcaagta cggtaaaaag cggtatcgaa tacatgacct
     1021 ggctggccgg ctggtaccgg gagaacagcg gcggcaaggg ggtcggtttt ttccagatcg
     1081 gcggcggcat tgccggcgac ttcccgatct gtgtggtccc catgatgtac caggacctgg
     1141 aatggcatga tgttccgttc tggagctatt tctgccagat ctcggactcc accacttctt
     1201 ttggctccta ttccggcgcc gtgccgaacg agaagatcac ctgggggaaa ctggatatcc
     1261 ataccccgaa attcattgtc gaaagtgatg ccaccatcgt agccccgctg atctttgcct
     1321 gggtgctggg gtggtaaatg tgaaatgtaa gagaataatt tatgaacgca aattcagaag
     1381 agctgtacct ggaggcggag gccgatatca agaacaacaa ttatgcggag gccttcaaga
     1441 aatatgagag catcctgtat gaggagcccg atagtgcgcc ggcgcacaac tccatgggct
     1501 ggctgtataa gacgcaattc gacgattacg caaaggcgga gaaccatttt cttaccgcta
     1561 tcaaatgcga cccgttgtac ccgcattcct attttcatat tgccgccctg ttgatggata
     1621 tggagcgctt tgacgagctg cggcagcatc tcgacaggtg tcttcgcgtc cggaccatcg
     1681 acaaatcctg ggtctatgga cgatatgccc tttccgaaga gcagcggcgg aactatgaga
     1741 aagccgtctt ctactttgag aaggcgatcc tcgtcagtca gaatgatgag aagatcaagg
     1801 attaccggca ggatatcgag aggtgccaga tgaagacgga catcgtcacc cggcacaagg
     1861 aacaagaaga aaaatagacc taccgctttg cgttaactcc attcttcctt atcccggtct
     1921 tcctgccttt tggatacgcg aacggatacg cggatgctga gttcatatag caggatcagc
     1981 ggcaatgcca cgatggtctg gctcatccag tccggtgaag gcgttatgat cgccgcgatg
     2041 aacaggatgg ccacataggc atatttacgg taggtgcgca ggaatttcgg agtgatgagc
     2101 ccgatcgccg tcaggacata gctgacgagg ggcaattgaa aggccaggcc cgagccgatg
     2161 atgatatcga caaggttttc gacatagtcg tcgagggttg gaatggtctt gatcgccccg
     2221 gcgcccccca gcgtatagtt gaaaaggaag ctgaatgtaa acggcgccag cagaaaatac
     2281 ccaaaggcca cacccagcaa gaagaagaag ctgacccaga agatcgcccc gcggctgctt
     2341 ttcagctctt ttggggaaag ggccggtttt acaaaacgcc agagttccca gaagacatag
     2401 gggaaggcca cgataaaccc gcctacaatg gcgatattga tgctggagat gaattgagag
     2461 ctgaattgcg tggtctggag ctgcaggccg gcaacggaat ccagacaaag ggcatccccc
     2521 atgtgtagtt tgcggcccag gctgcataag cctgtatagc tgacgaaatc cttgcggatg
     2581 ggtccccgga tgatcttatc gaagatccag ttcatcttta cgaagatgat aatggctata
     2641 ataacgatcg caaaggccga gcggacgata tgccagcgca gcgcctccag gtggtctaca
     2701 aaagacatct catcctcgcc ctgattcctg ttgaacagat ttaaagccat tgttttctaa
     2761 aaaagctccg cgaagttaac gggattgggc caaaaaatag cctcaaatat gttttttgcc
     2821 aattttggcc ggtttccggg gcgggagggg tataaaaaaa acaagcactc cattacagag
     2881 tgcattgttt actcctaact gacctatgaa aatcattgcc tccgggtgga ggttaaatat
     2941 tttttagcgt aagaccgtcg gttaggttgc tgtcttacag gacgaagatg cttaaa
//
LOCUS       DATGFG010001021         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1542430, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001021 DATGFG010000000
VERSION     DATGFG010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542430"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2872)
                     /gene="nifJ"
                     /locus_tag="VKQ52_19375"
     CDS             complement(<1..2872)
                     /gene="nifJ"
                     /locus_tag="VKQ52_19375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HLZ89429.1"
                     /translation="MGINPESTDSNLPKTKKADLAIMDGNEAAAYIAYKTNEVCAIYP
                     ITPSSTMGEWADEWSAQGLENIYGQVPKVIEMQSEAGAAGAVHGALQGGSLSTTFTAS
                     QGLLLMIPNMYKIAGELIPTVFHIAARSLATHALSIFGDHSDVMAVRSTGFSMLFGNS
                     SQEAMDMALIAQSATLSSRIPFLNIFDGFRTSHELTEVEKIPDATISAMIDMDEVNEH
                     RSRALSPDNPFIRGTAQNPDVFFQNREACNPYHNRVADIVKQKFNRFYELTGRKYMPF
                     DYYGHEEADRLIVIMGSGAGAVKETVDYLNGQGNKVGVLIVRLFRPFSIADFLAVIPT
                     SVKKVAALDRCKEPNSIGEPLYMDVVNAFSNARIRPYPLVIGGRYGLSSKEFTPAMVI
                     AVFEELDNPDPKQHFTIGINDDVTQLSLPYDHTFSLDKQHLFRGQFFGLGSDGTVSAN
                     KNSIKIIGTKTDNYIQGYFVYDSKKSGSLTVSHLRFGKHPIQSTYLVDSANFIACHHF
                     NYLNKYDILKDAVEGAVFLLNAPYPKEELWEKLPRPIQEEIIRKKLKFYAINAHAVAK
                     ESDMGGRINTILQTCFFAISNILPREEAIAKIKTAIFDTYGKKGEAVLQKNYEAVDKA
                     LANLYEVDYHAAYPGDTPLSNGVSDSAPAFVKDVLGKIIAGGGDELPVSAFPADGSYP
                     SGTTKWEKRNIADAVPVWDTGLCTQCNKCVLICPHAAIRAKVYDKALLADAPADFKHM
                     DPIGKEWNKEKEAYTLQVSTEDCTGCTLCMEYCPITSKTDPDHKAVNMVDKTGIQETE
                     RLNWEFFLHLPEVDRERINRNTVKGSQFFQPLFEFSGACAGCGETPYLKLLTQLYGER
                     MIVANATGCSSIFGGNLPTTPWSKNSEGRGPAWANSLFEDNAEFGLGIKLANEKKREA
                     ATSMLYQLKDKVGEELVEALVHTPETTEAEIQARTKLIRELKV"
     gene            complement(2988..>4011)
                     /locus_tag="VKQ52_19380"
     CDS             complement(2988..>4011)
                     /locus_tag="VKQ52_19380"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ89430.1"
                     /translation="DRPAGYDANRLVLIDGSVDLIRNYPALRNDLLATRLVRSATKSS
                     SPVNELWSWTGIKAWSGQLPSETLGLAEVDIADDYFKTLDMQFVAGHNFAGNLAADSL
                     NVILNEAAVKRMRYKNPIGQTITLYNLDKKIKVIGVVKDALMLSPFQPAEPTMFVYDP
                     NALNIAFRLAPGIDIHEAMQKFAPLFNRYNPAYPFLFHFADDSYAQKFQLEVLVGSLA
                     GLFAGLAIFISCLGLFGLAAYMAEQRTREIGIRKVLGASVSRLWLMLTKDFIILVAIS
                     CVVATPLAFYFMQNWLHQYPYRITIGPGVFLMAGVLALLITIITISFQAIRGATANPT
                     RSLRSE"
BASE COUNT          850 a          980 c         1153 g         1028 t
ORIGIN      
        1 gaaccttgag ttcccggatc agcttagtcc tggcttgtat ttcggcctct gttgtttccg
       61 gggtatgaac aagggcttcg acaagctctt cccctacttt atcctttagt tgatacagca
      121 tagaggttgc ggcctcccgc ttcttttcat tggcgagctt aatgccgagt ccgaattccg
      181 cgttgtcttc gaagagtgaa tttgcccagg ccggcccccg gccttcgcta ttctttgacc
      241 agggggtagt cggaagattg ccgccgaata tggaagaaca ccctgtggca ttggccacga
      301 tcatcctttc tccatatagt tgcgtcaaca attttaaata gggcgtttcg ccgcacccgg
      361 cgcaggctcc cgagaactcg aacaatggct gaaaaaattg ggatcccttt acggtattcc
      421 ggtttattcg ctctctgtcg acttcgggaa ggtgtaaaaa gaattcccag ttcagccgtt
      481 cggtttcctg gatgccggtc ttgtcaacca tattgacggc cttgtggtcc gggtcggtct
      541 tgctggtgat cgggcagtac tccatgcaaa gggtacaacc ggtgcagtct tccgtggaga
      601 cctgcagcgt ataggcttct ttttccttat tccattcttt gccgatgggg tccatgtgtt
      661 tgaagtccgc gggtgcgtca gccaataagg ctttgtcgta gaccttggcg cggatggcgg
      721 catgtgggca gatgagtacg catttattgc attgagtgca taaaccggtg tcccatacgg
      781 ggacggcgtc ggcaatattt cgtttctccc attttgttgt gccggacgga tagctgccgt
      841 ccgccggaaa agcgcttacg ggcagttcgt cgccgccgcc ggcaatgatc ttgcccagga
      901 cgtcctttac gaacgccggg gctgaatccg aaacgccatt gctcaagggc gtatcgccgg
      961 ggtaagccgc gtggtaatcg acttcgtata aattagccag ggccttgtct accgcttcat
     1021 aattcttttg caggacggct tcccccttct tgccataggt gtcaaagatc gctgttttta
     1081 tcttcgctat ggcctcttcc cgcggaagga tgttgctgat cgcaaagaag catgtttgca
     1141 gaatggtgtt gatacgcccc cccatatccg actcctttgc gaccgcatga gcgtttatgg
     1201 cgtagaactt cagtttctta cgtatgatct cctcctggat cgggcgcggc aatttttccc
     1261 acagctcctc tttgggatat ggtgcgttca gaaggaagac ggcgccctcc accgcatctt
     1321 tcaaaatatc atatttgttc aggtaattga aatgatggca ggcaatgaag ttggccgaat
     1381 cgaccaggta ggtggattgg atgggatgct ttccaaagcg gaggtgcgaa acagtcaatg
     1441 acccggattt cttcgagtca tatacaaagt atccctggat atagttatcc gtctttgtcc
     1501 cgatgatctt tatggagttt ttgttcgcac ttaccgtccc atccgagcca agaccgaaaa
     1561 actggcctct gaaaaggtgt tgtttatcca atgaaaacgt atggtcatag ggaaggctca
     1621 attgcgttac gtcatcgttg atgccgatgg taaaatgttg tttggggtcg gggttgtcca
     1681 attcctcaaa gacggctatc accatggcag gggtgaattc cttcgatgaa aggccatatc
     1741 ggccgccgat gaccagggga tagggacgga tcctggcgtt gctgaaagcg tttacgacat
     1801 ccatgtataa gggttcgccg atgctgtttg gttctttgca acggtcgagc gccgccactt
     1861 tttttaccga tgttggtatg acagccagaa aatcggcaat ggagaagggt ctgaacaggc
     1921 ggacgatcaa cacaccgacc ttgttcccct gcccgttgag atagtcaacg gtctctttca
     1981 cagcgcctgc tcccgagccc atgatgacga tcagccggtc ggcttcttca tggccataat
     2041 agtcaaacgg catatacttc ctgcccgtca attcgtagaa cctgttaaat ttctgtttta
     2101 cgatgtcggc caccctgtta tgatacggat tgcaagcctc cctgttttga aagaaaacgt
     2161 ccgggttctg cgcagtcccg cggatgaacg gattatcggg cgataatgcc ctgctcctgt
     2221 gttcattcac ttcgtccatg tcgatcatcg cgcttatcgt tgcgtccggg atcttttcga
     2281 cttctgtcag ctcatgcgag gtacgaaagc catcgaaaat attgagaaat gggatgcgcg
     2341 aactgagggt agccgactgt gctatcagcg ccatatccat tgcttcctga gacgaattac
     2401 caaacagcat gctgaagccg gtggaacgaa ccgccatcac atcgctgtga tcaccgaaga
     2461 tggaaagcgc atgggtggcc agagacctgg ctgcaatatg aaatacagtg ggtatcaatt
     2521 ccccggcgat cttatacata ttggggatca tcaggagcaa tccctgcgat gccgtgaagg
     2581 tggtagacag gctcccaccc tgcaatgctc cgtgtaccgc tccggcggct cccgcttcac
     2641 tttgcatttc gatgactttt ggcacctggc cgtagatgtt ctctaatccc tgtgcactcc
     2701 attcatccgc ccattcaccc atcgtggaag agggtgtaat gggatagatg gcgcagacct
     2761 cattggtctt ataggctatg taagccgcag cctcgttgcc atccattatg gcaagatctg
     2821 cttttttcgt ttttggcagg ttgctgtcgg tggactccgg attgatgccc atattcattt
     2881 gcttttatgg caataaatat aaacccggaa gtacggacag catatgaccg gggtcaggga
     2941 ttgggatgat tagtagcagg ttgcgggagg aggatggatg gcgccgacta ttccgaccgg
     3001 agacttcggg tcgggttggc ggtggcgccg cggatggcct ggaagctgat ggtgataatg
     3061 gtgatgagaa gggcgaggac gcctgccatg aggaagacgc cggggccgat ggtgatgcgg
     3121 tatggatatt gatgcagcca gttttgcatg aaatagaagg caaggggtgt agccacgacg
     3181 cagctgatcg ccacgaggat gataaagtct tttgtcaaca tgagccagag gcgggacacc
     3241 gaggctccca gtacctttcg gatgccgatc tcgcgggtcc tttgttcggc catataggct
     3301 gccaggccga ataatcccag acaggaaatg aagatggcca gaccggcgaa gaggccggcg
     3361 aggctcccca ccagtacttc cagttggaat ttctgcgcgt agctgtcgtc ggcgaaatga
     3421 aagaggaagg ggtatgccgg attgtaccgg ttgaacagcg gtgcgaattt ttgcatcgct
     3481 tcgtggatgt cgatgcccgg ggcaaggcgg aaggcgatgt tcaaagcgtt ggggtcgtag
     3541 acgaacatgg tgggttcggc aggttggaag ggagagagca tgagagcatc cttcactacg
     3601 ccgatgacct ttatcttttt gtcgaggttg tacaacgtga tcgtttggcc gatcggattt
     3661 ttgtagcgca tgcgttttac cgcggcttcg ttgaggatga cgttgaggct gtccgcggcg
     3721 aggttaccgg caaagttgtg gccggcgaca aactgcatgt cgagtgtttt gaaataatcg
     3781 tcggcgatat cgacttcggc gaggccgagg gtttcggagg ggagctgacc gctccaggct
     3841 tttatgcctg tccacgacca gagctcattg accgggcttg acgattttgt cgcggaccgg
     3901 acgagacggg tggcgagaag gtcattccgg agtgcggggt agttacgtat gaggtcgacg
     3961 ctgccgtcga tgagcacgag gcggttggca tcgtaaccgg cggggcgatc c
//
LOCUS       DATGFG010001022         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_869989, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001022 DATGFG010000000
VERSION     DATGFG010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869989"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VKQ52_19385"
     CDS             complement(<1..684)
                     /locus_tag="VKQ52_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89431.1"
                     /translation="MQKPAVFILSCLLLNTILRAQDTLPRFSATARGPGKILISWHNK
                     YRDVSQISIQRSADSLRNFTTLVTVPDPRLPENGATDNKAPHPNFYYRLFIVLDNGKY
                     LFTPSRRPHSQSEEPAVVKKEEEAEETIARAADTRMLVISPASEKSKIRTPTTIHNLP
                     ELSLSNTVFVRKGDTLLGQLSGTRVQAFRDSLLQRTKDTLVFIDGDTLLIKPFVAKEV
                     YRVSPYVFTG"
     gene            complement(713..1585)
                     /locus_tag="VKQ52_19390"
     CDS             complement(713..1585)
                     /locus_tag="VKQ52_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLZ89432.1"
                     /translation="MPEPTRQQIQVDVMRLDQLHPVISGNKWFKLQHHLSLATPGHPI
                     LTFGGAWSNHLVATACAAKQAGLAAIGIIRGERPAALSATLEETISYGMQLEFISRQQ
                     YKQKEDPAFLQDLSRRFPGAYIIPEGGGGLPGIKGSEDILRLTDTATYTHICCAIGTG
                     TTFLGLLRAAGSAQTVIGVPVLKGPESIEAIDTASLLTKEQSTRGRLLSGYHFGGYAR
                     HPQPLLDFMNRFYRDTSIPTDIVYTGKLFYAIEDSISRQYFPTHSRLLIIHSGGLQGN
                     RSLSPGKLPFGIGF"
     gene            1663..2562
                     /locus_tag="VKQ52_19395"
     CDS             1663..2562
                     /locus_tag="VKQ52_19395"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLZ89433.1"
                     /translation="MQPTLLILAAGMASRYGSMKQIQGFGPSGETIMDYSIYDAIRAG
                     FTKVVFIIRKDFADDFKSIFEPKLKGKVAIDYVYQDLHSFTGDFTVPAERTKPWGTAH
                     AVLCAKDAIKEPFAVINADDFYGRDAFEKAYKFLTGPVNGKTWCIIGYELAKTLSDNG
                     TVSRGVCQVDASGNLVSIAERTKIYEEGGKITYEEGDKKFEVPFDSKVSMNFWGFDAS
                     VFAYTEKLFKEFLKEQGTNPKAEFFIPIIGDKFIKEGKGDIKVIPTGAQWFGVTYKED
                     APEVQASLNALIAKGEYPDNLWK"
     gene            complement(2609..>2910)
                     /locus_tag="VKQ52_19400"
     CDS             complement(2609..>2910)
                     /locus_tag="VKQ52_19400"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HLZ89434.1"
                     /translation="FQLTIAANPGNSGTPVLNNDGEVIGIVTSSQQNAQGMVFAVRSK
                     NIFNALDAMKADSTLQKTDSTLSLLKIPVNSSLKGVDRKQQIKRIRDYIFIVKSN"
BASE COUNT          631 a          605 c          966 g          708 t
ORIGIN      
        1 gccggtgaac acatagggtg agacccggta tacttctttg gcgacaaagg gttttatgag
       61 cagggtatcg ccgtcgataa aaacgagggt gtcttttgtc cgttgcagca gggaatcccg
      121 gaaggcttgt acccgggtgc cggataattg accgaggagg gtatctccct tgcggacgaa
      181 gaccgtgttg gacagggaca gttcgggaag gttatgaatg gttgtgggtg ttctgatctt
      241 cgatttttcg gatgcgggtg agatgacgag catccgggta tcggcggccc gggcgatggt
      301 ctcttccgct tcttcttctt tttttactac ggcgggttct tcggactggg agtgcggacg
      361 tctggaaggc gtgaagaggt atttgccgtt gtcgagtacg atgaagagcc ggtaataaaa
      421 attgggatgg ggtgctttat tatcggtggc gccgttctcg gggagccggg gatccggtac
      481 ggtcaccagg gtggtaaagt tcctgaggct gtcggcggag cgctggatac tgatctggga
      541 aacatcccgg tatttgttat gccagctgat gaggatcttg ccgggaccgc gggccgtggc
      601 ggagaagcgg ggtagggtat cctgggcgcg gaggatggta ttgagaagaa ggcagctaag
      661 gataaatact gcgggttttt gcataagatg catgaaatta caaaatccgg gcctaaaaac
      721 cgatgccgaa agggagtttt cccggggaaa gggaccggtt gccttgtaaa ccaccgctat
      781 ggatgatcaa tagacgggaa tgggtgggga agtattgccg gctgatgcta tcctctatgg
      841 cgtagaacag tttgccggtg tagacgatat cggtgggtat actggtatcc ctgtagaacc
      901 ggttcataaa atcgagcagg ggttgcgggt gacgggcata gccgccgaaa tgataccccg
      961 aaagaagacg gccgcgggtg gactgttctt ttgtcaacag ggaggctgtg tcgatggctt
     1021 cgatgctttc cggtcctttt agaacgggta cgccgatgac cgtctgcgct gaaccggcgg
     1081 ctcgaaggag gcccaggaag gtagtgccgg tgccgatggc gcaacagata tgggtgtagg
     1141 tagccgtatc cgtgagtcgg aggatgtctt cgctgccctt gatgcccggc aggccgccgc
     1201 cgccttcggg aatgatatag gcgccgggga agcggcggga cagatcctgt aaaaaagcag
     1261 ggtcttcttt ttgtttgtac tgctgccggg agatgaattc cagttgcatg ccgtaggaga
     1321 tcgtctcttc gagcgtagcc gaaagggcgg cgggtctttc tccccggatg ataccgatgg
     1381 cggcgagacc ggcttgtttt gcggcgcagg cggtagcgac gagatggttc gaccacgcgc
     1441 caccgaaggt gagaatggga tgacctggtg tggcgaggga caggtgatgt tgcagtttga
     1501 accatttatt cccggatatg acggggtgga gttggtccag tcgcatgaca tccacctgga
     1561 tctgttgccg ggtcggttcc ggcaagtgca aacgttggca cagggatgat ggatgattca
     1621 ccttaatgct ttattttcgt gtccaaatat aaaaatagtc taatgcaacc cacgcttttg
     1681 atcttagccg ccggcatggc ctcccgctat ggaagtatga aacagataca gggatttggc
     1741 cccagcggag aaacgataat ggattattcc atctacgatg ccatccgtgc cggattcaca
     1801 aaggtagtgt tcattatccg gaaagatttt gccgatgatt tcaagagtat cttcgagccc
     1861 aaattaaagg ggaaagtggc gatcgattat gtctaccagg acctccattc gtttaccggt
     1921 gactttaccg ttccggccga gaggacgaaa ccctggggca cggcccatgc cgttctgtgt
     1981 gcgaaggatg cgataaagga gccttttgcg gtgatcaatg cggatgattt ttatggccgt
     2041 gacgcttttg agaaggcgta caagttcctg acgggaccgg tgaatggcaa aacctggtgt
     2101 atcatcgggt atgagctggc caagacgctg tcggataacg gcacggtgag ccgtggggtt
     2161 tgccaggtgg acgcgtcggg gaatcttgtt tcgatagcgg agcgcacgaa gatctatgag
     2221 gagggtggta agatcaccta tgaggaggga gacaagaagt tcgaagttcc gttcgattcg
     2281 aaggtgtcga tgaatttctg gggttttgac gcttcggtct ttgcgtatac ggagaaatta
     2341 ttcaaggagt ttttgaagga gcaggggacg aatcccaagg cggagttctt tatcccgatc
     2401 attggtgaca agtttatcaa ggaggggaag ggggatatca aggtgatccc caccggggcg
     2461 caatggtttg gggtgacgta taaggaggat gcgccggagg tacaggcgag tctgaacgcg
     2521 ctgatcgcga agggcgagta tccggataat ttgtggaagt aagtgatctt ttaaatagaa
     2581 gagcccgcgt tagcgggctc ttctattttt agttggattt gacgatgaag atataatcgc
     2641 ggatccgttt gatctgttgt ttgcggtcga ctccctttaa ggaggaattg accgggatct
     2701 tcagcaagct gagggtgctg tcggtctttt gcagcgtact gtcggctttc attgcgtcga
     2761 gggcgttgaa gatgttcttc gaacggacgg cgaagaccat tccctgggcg ttctgctggc
     2821 tcgaggtgac gatgccgatg acctcgccat cattgttcag gacgggggtt ccgctattgc
     2881 cgggattggc ggcgatggtg agctggaagg
//
LOCUS       DATGFG010001023         9779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_741635, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001023 DATGFG010000000
VERSION     DATGFG010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741635"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VKQ52_19405"
     CDS             <1..609
                     /locus_tag="VKQ52_19405"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HLZ89435.1"
                     /translation="GIFFEFIPASELGKENPQRLSLKDVTTGENYALVVSTNAGLWGY
                     SIGDMVRFTSVNPYRLVVSGRTKHFISAFGEHVIGEEVEYSLMKAAEEAGLHITEFTV
                     APMIRQEKGKSYHEWFVEFGNRPGDLAAFADKVDHNLREKNIYYDDLISGNILQPLKI
                     RPVKKNGFIDYMKSVGKLGGQNKVPRLSNDRKLAAELEKYTE"
     gene            624..1793
                     /locus_tag="VKQ52_19410"
     CDS             624..1793
                     /locus_tag="VKQ52_19410"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLZ89436.1"
                     /translation="MSNASVKKRIAIFGSTGSIGKQALEVIDANPGAFSVEVLTAQSN
                     DDLLIKQALHFKPNIVVIGDDKRYAHVKTALGSHPIKVFTGEKALEEVAAMDVYDIML
                     AAIVGFAGLRPTLKAIGNGKAIALANKETLVVAGDIVMRKALEKKAPIIPVDSEHSAI
                     FQCLIGETRNRIEKVILTASGGPFLGKKPNFLVNVKRDHALQHPNWNMGAKITIDSAT
                     LMNKGLEMIEARWLFDLKPDQVQVVVHPQSIIHSMIMFEDGSIKSQMGLPDMKLPIQY
                     ALGFPRRMPSSFPRYDFRKPNTLTFEEPDTKTFRNLLLATEALNKGGNLPCVLNAANE
                     IAVFAFLRNRIGFLDMTDLVERTMQEMPFIEHPTLEEYFESDGEARNFAASLIKL"
     gene            1871..3235
                     /gene="rseP"
                     /locus_tag="VKQ52_19415"
     CDS             1871..3235
                     /gene="rseP"
                     /locus_tag="VKQ52_19415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HLZ89437.1"
                     /translation="MYLLAIDWSAVGIKVLQFILSFSILVVLHELGHFLPARWFKCRV
                     EKFYLFFNPWFSLFKKKIGETEYGLGWIPFGGYVKISGMIDESMDKEQMQQAPQPWEF
                     RSKPAWQRLIIMLGGVTVNVLLAFAIFIMLMWIYGDNYVPVANLRYGLYADTLGRKMG
                     LQDGDNIIAVAGMPVERQGKVGSEMIMNEASTVTITRNGRQMELSVPPGLIGQLNKNR
                     LQGLAYARYPLVVESLLSNYKPLKGDVRKGDTLIGLEGHPVQFNVDLGKDSTFAFLKD
                     KKTRALTLRRGNDTVQALVAFDEKKGTGVHYKRMDSLLGSVHIPYTFWQAIPAGIHKS
                     GETLGDYIRNLKLLFTSREVKAQDSLGSVISIAGVFPPEWDWESFWTLTGIFSILLAF
                     MNVLPIPALDGGHALFTLVEIVSGRKPSDKFIEYAQMVGMVLLFSLMAYALGLDIFHA
                     FKFK"
     gene            3341..3713
                     /gene="ssrA"
                     /locus_tag="VKQ52_19420"
     tmRNA           3341..3713
                     /gene="ssrA"
                     /locus_tag="VKQ52_19420"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3828..5015)
                     /locus_tag="VKQ52_19425"
     CDS             complement(3828..5015)
                     /locus_tag="VKQ52_19425"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ89438.1"
                     /translation="MKKVFFLLFSSFLTYIHLLAQGTVLSGSVRDSATGKPMPGVSVF
                     LNSTSKGTITRADGTFTLGGISPGRYEVIISAVGYQTFNLAISTRNLPTDLKVALHVK
                     ASELTAIVVEPFMKDGWKKYGKFFIDNFIGTTENASSCKLKNKEVLRFHYYLKSRKLS
                     VTATEPLIIENKALGYDLEYRLERFECDFSTNIISYFGYPLFREMTTDYPQTRLEWEQ
                     RRRYAYLGSEMHFLRILYKGQLHQEGFIVEHEIEVPNIEKQRVKNIYKPNITKTDSIP
                     IDSLHLYWEVLRQPDFFIQKIKSTGDLLTIHPDQTRTMYFFGDCTVIYGNGRLGIAYQ
                     QSAINLLEPVSIEIGENGSYYPPTILSKGNWSKTETIANLLPRDYELVAPGTPSTTPP
                     PVR"
     gene            5675..5956
                     /locus_tag="VKQ52_19430"
     CDS             5675..5956
                     /locus_tag="VKQ52_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89439.1"
                     /translation="MEILLALFSFLWVNCVDIYQETDDNLRCYFYGNRFEVPPGCRVR
                     RVPFASVRSGLKFYSVGFISFRNETGNRRLKFFILADRDWHLIKERELS"
     gene            complement(6202..7449)
                     /locus_tag="VKQ52_19435"
     CDS             complement(6202..7449)
                     /locus_tag="VKQ52_19435"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HLZ89440.1"
                     /translation="MQCYGCYKPDVEGYCPACRKQLFDGKRVHTVLPFDAPTADNLEM
                     FQEKTKRLSISGVQLKYSLKLEGKELELTETGGQYILKPIPPSTQLVAREQAPENEHL
                     TMQIAAQLFGISTVANGLIYFKDGIPAYVTRRFDVRPTGGKYQQEDMAQLSGRSRNTH
                     GEHFKYDGTYEEIGRLIKKYVAAHLPALEQFFRLVIFNYLFSNGDAHLKNFSLIETDM
                     GDYTLSPAYDLMSTVLHTPLESDTALDLYKDSMESPFYSVFGYYGQANFRILADRIGI
                     IPLRRDRILTQLLSATELVSQMVEHSLLSEEAKKKYIDAYQDKVIRMGMTKDMIARAM
                     NPQYPGVYSVTDKPTKLTFLDRKVLTGFFDFTAQSDVLEKENKYTFIEMNNSERYRDT
                     RDMRYVTIVEGDMLQKVEYPAKG"
     gene            complement(7452..7778)
                     /locus_tag="VKQ52_19440"
     CDS             complement(7452..7778)
                     /locus_tag="VKQ52_19440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HLZ89441.1"
                     /translation="MAKAAIYNNGVLAGYLEKKGPEDYLFIYENNYWSDVSLPSISLT
                     LPKTHQTWRSPTLFPFFSGLLAEGVNKDIQCRLLKIDERDDFTRLLKTAGEDTIGAIT
                     VKEIQE"
     gene            complement(7778..7987)
                     /locus_tag="VKQ52_19445"
     CDS             complement(7778..7987)
                     /locus_tag="VKQ52_19445"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ89442.1"
                     /translation="MTVEELGKSIKFRREFLNLRQEDLAEMSGIAIKTIHLIESGSGN
                     PSVDTLGKIATVLGLELLLQIKKMN"
     gene            8485..9345
                     /locus_tag="VKQ52_19450"
     CDS             8485..9345
                     /locus_tag="VKQ52_19450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ89443.1"
                     /translation="MRKRMDLKNFNLFQYLKEQQSHGAQMPYFLANQDTYRKASIRFP
                     FRTFAYGIGLTYSGPGGVFRVGCTDYHTRSGSLITIGPGIVSQWMGNYTSIHDTIYFT
                     DELFKNNLRSSFLKSLSFFQPGGTHVIDLEEEYIRRIRAVFDLLRQCKEDSEVTSGLV
                     HSLISLTIRCHGMQKVKATLSVQEKIVSDFKILLSKFFLEKKDVAFYAQRLHITPKHL
                     SGVLQEETGKSAKTLINDHIFLEAKSLLRQTSMPVKEICHWLGFSDAAYFVRAFKRRE
                     GTTPQAYRKL"
     gene            9463..>9779
                     /locus_tag="VKQ52_19455"
     CDS             9463..>9779
                     /locus_tag="VKQ52_19455"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HLZ89444.1"
                     /translation="MAIQVSPFKIEVSETVLEDLATRLKHTRWTDEPADAGWRYGTNP
                     TYLKELVDYWQSRYDWRRQESMLNRLPQFRTAIDGVGIHFIHVRGKGKNPRPLLLTHG
                     WPDS"
BASE COUNT         2368 a         2441 c         2456 g         2514 t
ORIGIN      
        1 ggcatctttt ttgaattcat ccccgcatcg gaattgggca aggagaaccc gcagcggctt
       61 tccctcaaag acgtgacgac gggcgagaac tacgccctcg tcgtcagcac caatgccggg
      121 ctctggggct atagcatcgg cgacatggta cgcttcacct cggtcaatcc ctaccggttg
      181 gtcgtatcgg ggcggacaaa acacttcatc tccgcttttg gtgaacacgt cataggcgaa
      241 gaggtagagt acagtctgat gaaagcggct gaggaagccg gactgcacat cacggaattc
      301 accgtcgcgc cgatgatccg gcaggagaag ggcaagagct atcatgaatg gttcgtggag
      361 tttgggaacc ggcccggtga ccttgccgcc tttgccgata aggtcgacca caaccttcgc
      421 gaaaagaata tctattacga tgacctgatc tcgggcaata tcctccagcc gttaaagatc
      481 cggccggtaa aaaagaacgg cttcatcgat tacatgaaat cggtaggtaa attgggcggc
      541 cagaacaagg ttccccggtt gagcaacgat cggaaactgg ctgccgaact ggaaaaatac
      601 accgagtaac aaagagaatc actatgtcaa acgccagcgt aaagaaacgc attgccatct
      661 tcggctctac cggttccatc ggcaaacagg cgctggaggt catcgacgcc aatccgggcg
      721 cctttagcgt ggaggtcctg acggcccaaa gcaacgacga tctcctcatc aaacaggccc
      781 ttcacttcaa acccaatatc gtcgtcatcg gcgacgacaa gcgatacgcc catgtaaaga
      841 ccgccctcgg cagtcatccc ataaaggtct tcaccgggga gaaggcattg gaagaggtgg
      901 ccgcgatgga tgtatatgat atcatgctgg ccgccatcgt cggcttcgcc ggcctccgcc
      961 cgacactaaa ggccatcggc aacgggaaag ccattgcgct cgccaacaag gaaacgctgg
     1021 tggtcgcggg agacatcgtc atgcggaagg cgctggagaa aaaagcgccc atcatcccgg
     1081 tggactcgga acacagcgcc atctttcaat gtctcattgg cgagacccgc aaccgcatcg
     1141 aaaaggtcat cctcacggct tccggcggcc ccttcctcgg gaaaaagccc aacttcctcg
     1201 tcaatgtcaa acgggaccat gccctgcagc acccgaactg gaacatgggc gccaagatca
     1261 ccatcgactc ggccacgctg atgaacaagg gactggaaat gatcgaagcc cgctggctct
     1321 ttgacctcaa gcccgatcag gttcaggtag tggttcatcc tcagagcatt atacacagca
     1381 tgatcatgtt cgaggatggg agcatcaagt cgcagatggg actgcccgat atgaaactgc
     1441 ccatccagta tgcgctcggc ttcccgcggc ggatgcccag cagctttccc cgatacgact
     1501 tccggaaacc caatacgctc accttcgaag aacccgatac aaagaccttt cgcaacctgc
     1561 tcctggctac cgaggccctc aataaggggg gcaacctccc ctgtgtgctc aatgcggcaa
     1621 acgagatcgc cgtctttgcc ttcctgcgca accgcatcgg ttttctcgac atgaccgacc
     1681 tggtcgaacg taccatgcag gagatgccgt tcatcgagca tcccacgctg gaagaatatt
     1741 ttgaaagcga tggggaggcc cgtaattttg ccgcgtcgct aataaaactt tagcccgacc
     1801 gcaatttttt gccgaaattc gacatttaat aaaaattatc caccttgccg gtggctatcg
     1861 aaaaacaaat atgtacctac tagcaatcga ctggtccgcc gtcgggatca aggtccttca
     1921 gttcatctta tccttctcta tcctggtggt gctccatgaa ctgggtcact ttttgccggc
     1981 gcgctggttc aaatgtcgcg tggagaaatt ctatttgttt ttcaacccct ggttctccct
     2041 cttcaaaaag aagatcggcg agacggaata cggtctgggc tggatcccct ttggcggcta
     2101 tgtaaagatc tcggggatga tcgacgagag catggacaag gaacagatgc agcaggcgcc
     2161 ccaaccctgg gaattccgca gcaaaccggc ctggcagcgg ctgatcatca tgctgggcgg
     2221 cgtcacggtc aacgtactgc tggcctttgc catcttcatc atgctcatgt ggatctacgg
     2281 agataattat gtgcccgtcg ccaatctgcg atacgggttg tatgcggaca ctctcggccg
     2341 caaaatgggc ctgcaggatg gggataatat cattgccgtg gccggcatgc ccgtggaaag
     2401 acagggcaag gtcggttccg agatgatcat gaacgaggcc tcgaccgtta ccatcacgcg
     2461 caatggccgg cagatggagc tttccgttcc ccccggcctg atcggtcagc tgaacaagaa
     2521 caggctccag ggcctcgcct atgcccgcta tccgctggtc gtcgaatccc tcctgtccaa
     2581 ttacaagccc ctgaaggggg atgtccggaa aggcgatacc ctcatcggtc tcgaaggtca
     2641 tcccgtccag ttcaatgtcg atctggggaa ggacagcacc ttcgcgttcc tgaaggacaa
     2701 gaagacgcgt gcgctcaccc tgcggcgggg taacgacacc gttcaggcgc tggtggcctt
     2761 tgacgaaaag aaggggacag gcgttcacta caagcggatg gacagcctcc tcggttcggt
     2821 tcatatcccg tatactttct ggcaggcgat ccccgcgggc atccataaaa gcggcgagac
     2881 cctcggcgac tatattcgca atctcaagct gctcttcacc tcccgcgagg tcaaggccca
     2941 ggactcgctg ggcagcgtca tcagcatcgc gggcgtcttc ccccccgaat gggactggga
     3001 aagcttctgg accctcaccg gcatctttag tatcctgctt gcgttcatga atgtcctgcc
     3061 catcccggcc ctggacggcg gccacgccct cttcaccctg gtggagatcg tctccggccg
     3121 taagcccagc gacaaattca tcgaatacgc gcagatggta gggatggtgc tgctgttcag
     3181 cctgatggcc tacgccctcg gtctcgacat cttccacgcc ttcaaattca aataaacagg
     3241 cccgccctct gccggctcgc cgatggccgg gtcccaactt ttttgtatat ttgccgttcc
     3301 tcccgccgcc tcttacccgg cccgcgcggg gagattaact gggggtgcct ggttttgaca
     3361 gcatagatct ttgaaggtgt aagcatgtcg tgcgttggat aattagcacg ttaatctgaa
     3421 tattcaaact tcaaatggca atactcagta tgccatggct gcctaatcag tgattagata
     3481 gtcatttggg ccgtgctggc cggttcgccc gttaccacgc tgcaccgccg actcaaaaca
     3541 aacacgggat gctgcaacag taggctgtgc ctgttgctta agcccatcca gctaaggaag
     3601 cgattggttc gtccgtcccc ggccgcctcc cgacaatcaa tgacggaata aacatgtaga
     3661 aagctttcgg cgattatgtt tggacagggg ttcgagtccc ctcacctcca ctcctttttc
     3721 aagccgctga ttttcagcgg cttcttattc ttggttgatg agcaatcgaa ctccgtcagg
     3781 aaccgtccgt tttatgcctg ccctcagaaa ttggtacctc aagctgttta gcggacgggc
     3841 ggtggcgtag tggatggggt gccgggggcg accagttcgt aatcgcgggg gaggaggttg
     3901 gcgatggttt ctgttttgct ccagttgcct ttggagagga tggtgggtgg atagtagctg
     3961 ccgttctcgc cgatctcgat ggagacgggt tccaacaggt tgatagcgga ttgctgatag
     4021 gcgatgccta gtcttccatt tccatagatc accgtgcagt ctccaaaaaa atacattgtc
     4081 cttgtctgat caggatggat ggtgagcaga tcgccggtgc tctttatttt ctgaataaag
     4141 aagtcgggtt gtcttagtac ctcccaataa aggtggagac tatcgatggg gatgctgtct
     4201 gtcttggtga tgttgggctt atagatgttc tttacccttt gcttttcgat gttgggcact
     4261 tctatctcgt gttcgacgat gaacccttct tgatggagct gtcctttgta caggatgcgc
     4321 aggaagtgca tctcggaacc gaggtaggcg tatctgcggc gttgttccca ttccagcctg
     4381 gtttggggat aatcggtggt catttctcta aagagaggat aaccgaaata ggagatgata
     4441 ttggtgctga agtcacattc gaatctttcg aggcgatact cgaggtcata gccaagggct
     4501 ttgttctcga tgatgagagg ctcggtggcg gtgacggata gcttcctgct tttgaggtaa
     4561 taatgaaacc gaaggacttc cttattcttt agtttacaag aggaggcgtt ttcggtggtg
     4621 ccgatgaagt tatcgataaa gaatttcccg tatttcttcc acccgtcttt cataaatggc
     4681 tctacgacga tggcagttag ttcggaggct tttacgtgga gggcaacttt tagatcggta
     4741 ggcaggttgc gggtgctgat ggcgagatta aaggtttggt aaccgacggc ggaaataatg
     4801 acctcgtatc tgcctgggga aatgccgccg agggtaaagg tgccgtcggc gcgggtgatg
     4861 gtgcctttgg aggtactgtt gaggaatacg ctgacgccgg gcatgggttt gccggtggcg
     4921 gaatcccgca cagagccgga caggacggtg ccttgggcca gaagatgtat ataggtcagg
     4981 aaggaggaga acaggaggaa aaaaaccttt ttcatggagg attttagacc cgcaaaataa
     5041 gatataaagc gatcttgcta aaaagtcgct ttaatgataa acgttatccc aatggagata
     5101 gctgccccta tgatcatgca acaaaataag tacatccgcc agatagcgct gacgctttta
     5161 ctgggaggca ttccaaaaat cttgaaaata gacttcctgc ctaccaggac aataaggagg
     5221 gtggcaatga cccagaggat ggcaaatatg gggtagtcca tgaccgtaat cgtttgaata
     5281 aaggtacaat ttattttttg acagggtcaa aaccagcaaa aagccggtaa ggtgttcgaa
     5341 tagaaccctc gtcacctcca ccgctcttca agccgctgat tttcaccggc tttttttgaa
     5401 tgctggcggg gaatcaaacg ttgccctgtt gcatacaatg ctgctacggc cctgggagag
     5461 ccgggcgata aagtgatgaa aaatttcgtc gaaagaaggg ctaatagaaa atattttcta
     5521 atttgtttgc tggatgaaac ctctccctgc catgcgaatt acttcccgga tttacatgtt
     5581 atttgcgctc gtgtgtctta ttgctgtcct tgcgctccca atagcgtttt ttcaggactc
     5641 aaaggacgat cgatcggttt ggctatttat cggcgtggaa atccttcttg cgcttttctc
     5701 ctttctttgg gtgaattgcg tcgacatcta tcaggagacg gatgacaacc tacgctgcta
     5761 tttttatggc aaccggttcg aagttcctcc cggctgtcgt gtccgcaggg tgccttttgc
     5821 gagtgttcgc tccggtctca agttctatag cgtgggcttt atttcatttc gaaacgaaac
     5881 ggggaatcgg cggcttaaat tcttcatcct cgccgaccgc gactggcacc tgatcaagga
     5941 aagggagctg tcatagaaag aggagcggat gggcttgtac agcgacccga aatctcctat
     6001 acaagagccc tggatctatg atccctgtag atattcaaat ctaatattta attcattgta
     6061 taattgtaaa cagcacgatc aggggttcga tccccacccc ctcacctcca cctgcatttc
     6121 aaagccgctg attgtcagtg gcttttgttt tgggaaccgt cactagatca aactcaattg
     6181 cctgccgggt cttatcatta tttacccctt tgctggatat tccacctttt gcagcatatc
     6241 accttctaca atcgtcacat atcgcatatc tcgcgtgtcg cgataccgtt cggaattatt
     6301 catttcaata aatgtatact tgttttcttt ttccaggacg tccgattgag cagtgaagtc
     6361 aaaaaatcct gtcaatacct tcctgtccag gaaagttaat ttcgttggtt tatcggttac
     6421 cgaatatact cccgggtatt gtgggttcat cgcccgggca atcatatctt ttgtcatgcc
     6481 catcctgatt actttgtcct ggtaagcgtc tatgtacttt ttttttgctt cttcacttaa
     6541 taaggagtgt tcgaccatct gcgaaaccag ctctgttgcg gataatagtt gagttaagat
     6601 cctatcccgt cttaagggta ttattccgat tctgtctgcc agtatacgga aattggcctg
     6661 tccataatat ccgaatacac tgtagaatgg actctccata gagtctttgt ataagtccag
     6721 ggccgtgtct gattccagcg gcgtgtggag gaccgtggac atcaggtcat acgctggcga
     6781 taatgtgtaa tcgcccatgt cagtctctat cagcgagaaa ttcttaagat gcgcatcccc
     6841 atttgaaaaa aggtagttga atatgactaa tctgaaaaat tgctccaagg cgggtagatg
     6901 ggctgcgaca tattttttga tcaatcgacc aatttcttca tatgttccat catacttaaa
     6961 atgctcgcca tgtgtattcc gactgcggcc gctaagctgg gccatgtctt cctgttggta
     7021 tttgccgccg gtaggcctta catcaaaacg ccgtgtcacg tatgccggta tgccgtcttt
     7081 aaaatagatc aggccgttag cgactgttga gatgccaaag agttgagccg cgatttgcat
     7141 ggtcaggtgt tcattctccg gagcctgttc ccgggcaaca agctgggtag aagggggaat
     7201 tggctttaat atatattggc cgcctgtttc ggtcaattca agctccttcc cttccagttt
     7261 gagggaatac ttcaattgaa caccagaaat ggagaggcgt tttgtttttt cctggaacat
     7321 ttccagattg tctgctgttg gtgcatcaaa gggaaggaca gtatgtaccc gcttgccgtc
     7381 aaaaagttgt ttgcggcaag cggggcaata gccctcgaca tccggtttat agcatccgta
     7441 acattgcatg gttattcctg tatctcttta acagttattg cacctatggt gtcttcgccg
     7501 gctgttttca gcaggcgggt aaaatcatcc cgttcatcta ttttcagcaa gcgacactga
     7561 atatctttat ttactccttc tgccaacagg ccggaaaaaa aaggaaacaa tgtagggctt
     7621 cgccatgttt gatgtgtttt gggaagcgtc aggctgatcg atgggagaga gacgtctgac
     7681 caataattat tctcatagat gaataggtag tcctccggtc cctttttttc caggtacccc
     7741 gccaatacgc cattattata gatcgctgcc tttgccatca attcatcttc ttaatttgca
     7801 gtaacaactc caacccaaga accgttgcaa tttttcccaa agtatccact gatgggttac
     7861 cagagcccga ttcaatgaga tgaatcgttt ttatggcgat cccgctcatc tcagccaaat
     7921 cctcctgccg gaggtttagg aattcccttc ggaatttaat tgatttacct aattcttcca
     7981 ctgtcatcgg taatatattg ctggtttcta gcaaaagtaa tataaatatt tccgaaaaag
     8041 ccgataatcg gcaatatttt actgatttgt tgaagggtac ctcagatatc cttttctttc
     8101 tggctatatc agtaatatat ttccgaatag ataggccatt tagccgggtt cattgaactc
     8161 ccctcacctc cacgaaaatt tcggccctgc aaatcaatga cttgcagggc cattttgttg
     8221 atagttcaag ggaatatcga acttctgcat aaattcgacc caaagaactt taactgtcct
     8281 tcataggcat atctgccagg aatatgcgac tgtacttgct tgaaacccgg cgtggatctc
     8341 gggtgcttca tgccctcagg tttttttcaa aaagacacgc taaattcaaa atgaacctga
     8401 tcgccatttc atcgcacatg tgccgttgaa ctggccgatg cgtttaccca aaagcatata
     8461 gatattgttt ataagaagtt agttatgaga aagagaatgg atttgaagaa ttttaacctc
     8521 tttcagtatc tgaaagagca gcaatcacat ggcgcgcaga tgccttattt cctggcaaac
     8581 caggacacct atagaaaagc atcgatccgg ttccccttcc gaacctttgc ctatggcatt
     8641 gggctcactt attccgggcc gggtggtgtg ttcagggttg gatgcaccga ctatcacaca
     8701 cgatcgggca gccttatcac cattggccca ggcattgttt cgcaatggat gggtaattac
     8761 acctccatcc atgataccat atatttcacc gacgagcttt ttaaaaacaa cctgcgatcc
     8821 tcttttctca aatcattatc ttttttccaa cctggcggta cgcatgtgat cgatctggaa
     8881 gaagagtata tcagaagaat aagagcagtt tttgacctgc tcagacaatg taaggaagat
     8941 tcggaggtga ccagcggcct ggtccattcc ctgatttccc ttactattag gtgtcacggg
     9001 atgcaaaagg taaaagcgac actatccgta caagaaaaaa tcgtaagcga ttttaagatc
     9061 cttttgtcga aattcttcct ggaaaagaaa gatgttgctt tctatgccca acggttgcat
     9121 attacgccga aacaccttag cggggttttg caggaggaga cgggtaaatc agcgaaaacg
     9181 ttgatcaacg atcatatctt tttggaggca aaaagcctgc ttagacagac aagtatgccg
     9241 gtgaaagaga tatgtcactg gctgggattt tctgacgcgg cttattttgt cagagcattt
     9301 aagagaagag agggcactac gccgcaagcg tatcgtaaac tatgagctac ctcgttacag
     9361 ggagcgcccg gtgtgcaaaa agtaccattt ttcccgactt tcagacaatc ccccggggcg
     9421 cctccagcgt atttttgtga aactacaact caaatcgctc ctatggctat tcaggtatct
     9481 ccctttaaaa ttgaagtgtc ggaaacggta ttggaagacc tcgcgacacg attaaaacac
     9541 acccggtgga cggacgagcc tgctgatgcc gggtggcgct acgggacaaa cccgacatat
     9601 ttgaaggaat tggtagacta ttggcaaagt cggtatgact ggcgcaggca ggaatctatg
     9661 ctgaaccggc ttccgcagtt caggacggcg atagacggag ttggcattca ctttatccat
     9721 gtcaggggaa aggggaaaaa ccccagacca ttgctgctca cccacggctg gcccgattc
//
LOCUS       DATGFG010001024         4923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1311215, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001024 DATGFG010000000
VERSION     DATGFG010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311215"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VKQ52_19460"
     CDS             complement(<1..662)
                     /locus_tag="VKQ52_19460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HLZ89445.1"
                     /translation="MRVHVLQHVLFEGPGSIADWCVAGGHALSVTPLYSAGGVLPDVE
                     VVDLLVIMGGPMSVYDDEVHPWLATEKEFIRAFLETEKPVLGICLGAQLLAVCCGARV
                     GRAIHTEIGWFPVSPTPAAAAFPWLDSLLASRPVLFHWHGDRFEIPAGAENLVSTEAN
                     DNQAFVLLGGRVVGLQFHPEMTPVLLEQMLAEGRHELRVSDFVQTEDSMITGAEYRDA
                     GGV"
     gene            complement(659..1240)
                     /locus_tag="VKQ52_19465"
     CDS             complement(659..1240)
                     /locus_tag="VKQ52_19465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLZ89446.1"
                     /translation="MKRIVVFCGSSSGTDAVYEARAYETGAVLAKSGIGIVYGGARVG
                     LMGAVADGALAHGGEVIGVLPRFLSGKEIGHNGLTDLILVDTMHERKTKMHELADGVI
                     TLPGGFGTMEELFEMLTWGQLGLHMKPVGILNISGFYNELIDLLQTMVDKGFLKPENQ
                     RMLLFADTPEELLAKMRRYQAPAAPKWITKETT"
     gene            complement(1599..2888)
                     /locus_tag="VKQ52_19470"
     CDS             complement(1599..2888)
                     /locus_tag="VKQ52_19470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HLZ89447.1"
                     /translation="MTATKNQNAIYIIGALFFIYGFITWLNSTLIPYLKLVCELKNYV
                     QAFFVTFAFYMSYLVLALPASYVLKITGFKKGMALGLAILGIGALLFIPAARQRSFPL
                     FLFGLFVQGGGLSLLQTASNPYVSILGPMESAARRISIMGVCNKSGGILSPLILGTLL
                     LQGTDAIRNQVEKTIDAATRERLLMDLSGRVVAPYLVMGIALIVLAFLLLRSGLPEIE
                     TEQSVVGEVSGVAETKTSIFDFPQLWLGVICLFVYVGVEVMAGDAIAVYGQSEGMTIS
                     TTKYFSSFTLGSMLAGYLVGIVCIPKMFSQQMGLRVSALLGLVFTTLAYFTAGYSAIT
                     FIALLGLANALMWPAIWPLAIEGLGRHIKTGAALLVMGIAGGAVIPLLYASLKDHPGI
                     GNKLAFFYCVFPCYVYIFYYAVWGYKRRKQRLRAALA"
     gene            3066..3914
                     /locus_tag="VKQ52_19475"
     CDS             3066..3914
                     /locus_tag="VKQ52_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89448.1"
                     /translation="MSPKLALGFLLAASATLAKGQNNPPDSIRDSYHIFFPAMGKDVL
                     AAHIGDHSYKIVNLYNKPSEVYVDNKLIGEGELSKYNDIIQRIKASVKDDDEAREMRE
                     MERDRRQAERDQRQAERDREQEAKDREQHQRDRAQEQIDREQSRRDQEQSRRDHEQSR
                     RDREQSQRDRERIDQDREQSRQGDDGCDDCDDQAEQQQREQSAEDRAMLKKGIQVLVD
                     EHIINNPQSLRTLVLTDTDISVNGIKQSPNVYQEIRAKLGDWARSGLSYGAEGPADNY
                     SLTIND"
     gene            4186..>4923
                     /locus_tag="VKQ52_19480"
     CDS             4186..>4923
                     /locus_tag="VKQ52_19480"
                     /inference="COORDINATES: protein motif:HMM:NF036995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HLZ89449.1"
                     /translation="MGDSTRGHQVLNYDGLLQKISSSYINAQASAVSAVNVAVLKAYW
                     EIGKYIVEYEQEGSLKAAYGKKLLKHLSKDLSLLHGKGFSHSNLQRMRQLYALYPIYA
                     KPSHKLSWSHYVELLKIDNPLERNFYEKQAILEHWSVPELKRQKQASLFLRLAASKDK
                     KGIKELSEKGQIVQHPTDLFREPYILDFLKIPEPAQLSESDLETSLLNNLQNFLLELG
                     KGFAFIGRQYRITLGNRHHRVDLVFYHR"
BASE COUNT         1323 a         1508 c         1100 g          992 t
ORIGIN      
        1 acaccccccg catcccggta ttccgcgcct gtaatcatcg aatcttccgt ttgtacaaaa
       61 tcgcttaccc ggagttcatg cctgccctcc gccagcatct gctccaaaag caccggcgtc
      121 atctccggat ggaactgcaa cccgacgacg cgcccgccca aaagcacgaa ggcctggtta
      181 tcattggcct cggtcgacac caggttctcc gcgcccgccg gtatttcaaa cctgtcccca
      241 tgccagtgaa aaagtacggg ccggctcgcc agtagcgaat ccaaccacgg gaaagccgcc
      301 gccgccggag taggcgacac ggggaaccat ccgatctcgg tatgaatggc cctgccaacc
      361 ctcgctccgc aacaaaccgc cagcaattgc gccccaagac agatcccaag cacgggcttc
      421 tctgtctcca aaaaagcccg gataaattct ttttccgttg ccagccaggg atgaacttcg
      481 tcgtcataaa cactcatcgg gccgcccatg atcaccaaca gatcgaccac ctcgacatcc
      541 ggcaacacgc ccccggccga gtaaagcggc gtaacactca gtgcatgccc accggcgacg
      601 caccaatcgg caatcgaccc cggcccttca aaaaggacat gctgtaatac atggaccctc
      661 atgtggtctc ttttgtgatc catttgggcg ctgccggcgc ctgataccgc cgcatcttcg
      721 caagcaactc ttcgggcgta tcggcaaaaa gcagcatccg ctggttctcc ggtttaagaa
      781 accccttgtc caccatcgtc tgtaagagat cgatcagctc attataaaat ccggaaatat
      841 tcaggatgcc gacgggcttc atatgcaagc ccaattgtcc ccaggtcaac atctcaaaaa
      901 gctcttccat tgtcccaaaa ccaccgggta gcgtgatcac tccatccgca agttcatgca
      961 tcttcgtctt gcgctcatgc atggtatcga caaggatgag atcggtaagc ccgttatgcc
     1021 cgatctcctt cccactcaga aaacgcggca ataccccgat cacctctccg ccatgggcca
     1081 atgccccatc ggccacggca cccatcaaac ccacccgcgc gccgccatag acaataccaa
     1141 ttcctgattt ggccaaaacc gccccggtct cataggcccg cgcctcatac acggcgtccg
     1201 ttccggaact cgatccgcaa aaaacaacga ttcgtttcat atggctaaag ttcggccttc
     1261 ttcgccagaa aacacttctc ccatcactca tctgcctgcg ctaatcgcag aacattgatt
     1321 gtcaatgcat aaaaaagcat gaaaaaaatc agggatttgt agttacaaaa ctacaatcgg
     1381 ctagtactaa ttctatataa agcaatagat aattcaggtt tcatcttcat aagcgcacat
     1441 tcggattttg ggttttttgg atttcggttt tcggttttcg ttgcgggcca tcacttacat
     1501 gggtaagggt ggtctttttt tatgccgcgt cccgggatca ctctcctcct cagataacat
     1561 ccaccatggg cgcacccgct ttatctgacg ccggcggact aggccaaagc cgcacgtagc
     1621 cgctgcttcc tccgcttgta cccccagacc gcgtagtaaa agatatagac atagcagggg
     1681 aacacgcagt aaaagaatgc cagcttattc ccgatacccg gatggtcctt cagcgaagca
     1741 tatagcagcg ggatcaccgc cccaccggca atccccatca ccagcagcgc cgctcccgtc
     1801 ttgatatgcc gtcccaaccc ttcaatcgcc aggggccaga tcgccggcca catcagcgca
     1861 ttggcaagac ccagcagcgc gataaaggtg atcgccgaat accctgccgt aaaataagcc
     1921 agcgtcgtga acaccagccc cagcaatgcc gagacccgta ggcccatctg ttggctaaac
     1981 atcttcggga tacaaacgat cccaaccagg tagcccgcaa gcatcgaccc caatgtaaag
     2041 cttgaaaaat atttggtcgt ggagatcgtc atcccctccg actgaccata gaccgcgatc
     2101 gcatctcccg ccatcacctc tactccgaca tatacaaaca aacaaatgac cccgagccaa
     2161 agctggggaa agtcgaaaat actcgtcttc gtttccgcca ccccggaaac ctctcccaca
     2221 acggattgct ccgtctctat ctccggtaat ccgctccgca acagcagaaa cgccagcacg
     2281 ataagcgcaa tacccatcac aagataaggg gcgaccaccc tgcccgaaag gtccatcagc
     2341 aatcgctccc gggtcgccgc gtcgattgtc ttttccacct ggttgcggat cgcatcggtc
     2401 ccctgcaaga gcagggttcc caggataagc ggactaagga tcccgccgct cttattacag
     2461 acacccatga tactgatccg cctggcagcg ctctccatcg gccccaggat actcacatag
     2521 ggattggacg ccgtctgtaa cagcgaaaga cccccgccct gtacaaaaag gccgaaaagg
     2581 aacaagggaa agctgcgttg ccgggcagcc gggataaaca acagtgcccc gatccccaga
     2641 atggccaacc ccagcgccat ccccttttta aagcccgtga tcttcaggac ataggaggcc
     2701 ggcaaggcca gcacaaggta ggacatgtaa aaagcgaagg tcacaaaaaa ggcctggaca
     2761 tagttcttca gctcacacac aagtttcaga taggggataa gcgtcgaatt gagccaggta
     2821 ataaagccgt atatgaagaa cagggccccg ataatataga tcgcgttttg gttcttggtt
     2881 gcagtcatag actacaaaat actccctttt taccggcttt ttcaacgcaa acgattgtcc
     2941 ctgtccggcc aacgcaaaca attgtcccgg tccgcactgc cgtcgccccc cgcaaaataa
     3001 ttctccccct tcctgtaacc tttggccatt tcggaagtat cctatatagc aaaaagctaa
     3061 aaaccatgag cccgaaactt gccctcggat tcctcctggc cgcatcggcc accctcgcca
     3121 aaggccagaa caatcccccc gactccatcc gggatagcta tcatatcttc ttcccggcca
     3181 tgggaaagga tgtcctcgcc gcgcatatcg gcgatcatag ctataagatc gtcaatcttt
     3241 acaacaagcc atcggaagtt tatgtcgaca ataagctcat cggggaaggg gaactctcaa
     3301 agtacaacga tattatccag aggataaaag cttcggtcaa agacgacgac gaggcccgcg
     3361 aaatgcggga gatggaacgc gacagaagac aggccgaacg cgaccagcga caagccgaac
     3421 gggatcgcga gcaggaggca aaggatcgcg aacaacatca gcgcgatagg gctcaggaac
     3481 agatcgaccg cgaacagtcc cgccgcgatc aggagcagtc ccgtcgcgac cacgaacaat
     3541 cccgccgtga ccgcgaacaa tcccaacgcg atcgggaacg aatcgaccag gaccgggaac
     3601 aatcccgcca aggcgacgac ggctgcgacg actgcgacga ccaggccgaa cagcaacaac
     3661 gcgaacagtc cgccgaagac cgtgccatgc tgaaaaaagg catccaggtc ctcgtcgacg
     3721 aacatatcat caacaatccc caaagtctca ggaccttggt gctcaccgac accgacatct
     3781 ccgtcaacgg catcaaacaa tccccaaacg tttaccagga gatccgcgcc aaactcggtg
     3841 actgggcccg cagcggcctc tcctacggcg ccgaaggccc cgccgacaac tactccctta
     3901 ccataaacga ttaatacact atcggccttc ccactcccga aggcccccgc tcatccaaaa
     3961 cgctgccacg cacaactgca gccacccacg ccggatactc caacatatcc gaagttttat
     4021 ttcgtccttt caccgggata acaaaacttt cctaaatttg catacctcct ctgctaagtt
     4081 tccctttgtg tttttcaact agtacttaac ggcaggacca gatcggtcct ggtggcctcc
     4141 aaaaggcaag taacgggtgg gttaaacatc ttaaaggtta cttccatggg agacagcaca
     4201 cggggtcatc aggtcctcaa ttacgacgga ctcttacaaa aaatctcatc cagctacatc
     4261 aatgcacagg caagcgctgt gagcgctgtc aatgtcgcag tactcaaagc ctattgggaa
     4321 atagggaaat atattgttga atatgaacaa gaaggaagct taaaggcagc atacggtaag
     4381 aagctcctga aacatctttc caaagacctt tccctgctgc atgggaaagg gtttagtcac
     4441 agtaatttgc agagaatgag acagttgtat gcgttgtacc caatttatgc gaagccttcg
     4501 cacaaattga gctggtctca ctatgtcgag ctcctgaaaa ttgacaaccc attggaacgg
     4561 aacttttacg aaaaacaagc cattctggaa cactggtcgg ttccggaact aaaaaggcaa
     4621 aagcaggcgt ccctctttct tcgattggct gcctcgaagg ataagaaagg cattaaggaa
     4681 ttgtccgaaa aaggtcagat cgtgcaacat ccaaccgatc tttttaggga gccctatatt
     4741 cttgattttt tgaaaattcc cgagccggct caattgtcgg aaagcgatct cgaaaccagc
     4801 cttttgaata acttgcaaaa cttcctgctt gaacttggaa aaggctttgc ttttatcggg
     4861 cgacaatatc gcataacatt gggaaatcgg caccatcggg ttgaccttgt cttttatcac
     4921 cgg
//
LOCUS       DATGFG010001025         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1079724, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001025 DATGFG010000000
VERSION     DATGFG010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079724"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VKQ52_19485"
     CDS             complement(<1..488)
                     /locus_tag="VKQ52_19485"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89450.1"
                     /translation="MDISQHLNIASTEEDIEPFLEVAPEIVQKRRSQGRIIFDRFIRH
                     RPAIVGACFLILLFLFCFAGPIVTTHNQPDLIHVTNVSQTLAGPSWQFPFGTDDTGRD
                     MMARAMAGGRVSLLVALTSMLVALVLGVGIGAFAGFYGGIVDTLLMRFTDVILSVPLY
                     LLL"
     gene            complement(525..1487)
                     /locus_tag="VKQ52_19490"
     CDS             complement(525..1487)
                     /locus_tag="VKQ52_19490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89451.1"
                     /translation="MGRYIIRRLLQALLLLALLSVGMFILIHALPGGPEQVIFNPRLS
                     AAGRAAIRARFGLDQPLYVQYLKWLGNAFVGNFGFSFVTNQPVSELLAERFPATLQLF
                     SLALLLALILAIGIGVISAVRQGSVTDYSLTTLSYFGIAMPVFLLGLFAQDIFGVWLH
                     LLPVSGTATLGYTFDPFNAFLDHLEHLVLPMLVLAVAFIAGWSRYMRASMIEVTKQDY
                     MRTARAKGVAPVSMLLRHALRNAVIPLITVVALDFGQVAGGATITEGVFAWPGMGLLF
                     LDSLTNRDYPVLLAMLMISATFVIGFNLIADLLYAVMDPRIRYS"
     gene            complement(1707..>2889)
                     /locus_tag="VKQ52_19495"
     CDS             complement(1707..>2889)
                     /locus_tag="VKQ52_19495"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLZ89452.1"
                     /translation="SQPFVAFNTVWADALFAPLPAHHFSSMSPDAILKSPDNLNPSVT
                     DGPFMMSESKPGDHFTVVRNPKYYRASEGLPYLDKIVFRLIPDQNTILKDFQAGSITS
                     AWFLDVTKVPAYKALANYHLVSNPNSSNFEAMYFNLDKPALKDVTVRKAISMAIDYNA
                     LIQTARRGEAVPLCTDHGKAYVPGYQANITCPKFDPAAANALLDQAGYTKGADGVRTK
                     NGLRLEFKYSTTANNAWRAADETILQQDLQAIGIKLDIQNYSADTYFGTVLPQGNFDI
                     GEFEDSWTYDADDSSLFACNQIPSAANSFGGGNYSRYCNKTLDGLLQQELSSGDPNVR
                     QQVFNQEHQIYLTDFPFITLYGPTDIAVVKNTTNNYLPGPEGASETIGVWNWWCTNGQ
                     C"
BASE COUNT          706 a          748 c          878 g          557 t
ORIGIN      
        1 aggagcaggt acagcggcac cgagaggatg acatcggtaa aacgcatgag cagggtatcc
       61 acgatgccgc cgtagaagcc ggcgaaagct ccgatgccga ctccaaggac cagcgccacg
      121 agcatcgagg tcaaggccac cagcagcgag acgcggccgc cagccatggc gcgggccatc
      181 atatctcggc cggtatcgtc ggtgccaaag gggaactgcc agcttggtcc ggccagggtt
      241 tgagagacgt tggtcacatg aatgaggtcg ggctggttat gggtggtcac gatggggccg
      301 gcgaaacaaa acaggaagag gaggatgagg aagcaggcgc ccacaatggc gggccgatgg
      361 cggatgaaac ggtcaaagat gatgcgtccc tggctgcgcc gcttctggac gatctctggg
      421 gccacttcca gaaatggttc gatgtcttcc tcggtcgaag cgatgttgag atgttgggat
      481 atatccactg gtttctcctt ctttcagaag tagtagatat cacactatga gtaacgaatg
      541 cgcgggtcca tcacggcata gagcaagtcg gcgatcaggt tgaagccaat cacaaacgta
      601 gcactaatca ttaacatggc taacaggacc ggatagtcac gattggtcag tgagtccaga
      661 aaaagcaagc ccatgccagg ccaggcgaaa acaccctcgg taatcgtcgc gccgccggcc
      721 acctggccaa aatccagagc cacgacggta atcagcggga tgacggcgtt gcgtaaggca
      781 tggcgaagga gcatggagac cggcgcgacg cccttggccc gggcggtgcg catgtagtcc
      841 tgcttggtca cctcgatcat actggcacgc atatagcgac tccagccggc aatgaaggcc
      901 acggccagca ccagcatcgg taaaaccagg tgctccaggt gatcaagaaa cgcattgaag
      961 ggatcgaagg tatagcccag ggtggcggtt cctgacactg ggagcaggtg taaccagacg
     1021 ccaaagatat cctgcgcgaa gagaccaagc agaaagaccg gcatggcaat cccaaagtag
     1081 gacagcgtcg tcaagctata atcggtcacc gacccctggc gcaccgcgga gatcactccg
     1141 atgccgatag ccaggatcaa cgccagcagc agtgccaggc taaacaactg cagagtcgcg
     1201 ggaaaacgct cggccagaag ttctgacact ggttggttgg taacaaacga gaagccaaaa
     1261 ttaccaacga aggcattgcc cagccatttc aagtattgaa cgtagagcgg ctgatccaga
     1321 ccaaaacggg cgcggatggc cgcacggccg gctgcgctga ggcgagggtt aaagatcacc
     1381 tgttcgggtc cgccgggtaa ggcgtggatt aagataaaca tgcccacgga taacaaggcc
     1441 agcaacagga gagcctggag caagcgtctg ataatatatc gtcccatttt tacctccgac
     1501 gagcatcaag ttaaggaaga ggacggctga cgttggatac agtgtaccat acttctatac
     1561 atttttacta gcgctttgcg catacaggca aggttttatt agaacggtac aagaaggcct
     1621 ctgactggac acccttctcc tcaagaaacg aatccaccca gaggccactt caccacccct
     1681 aactccgtag cacaggctac tttacgctaa cattgaccgt tagtacacca ccagttccag
     1741 acgccgatgg tctcggaagc tccctcgggt cctggcagat agttgtttgt ggtattttta
     1801 actaccgcga tatctgtggg tccgtagagg gtgatgaacg ggaaatcggt caggtaaatc
     1861 tggtgttcct ggttgaagac ctgttggcgg acattgggat ccccactgga gagctcttgc
     1921 tggagcagcc cgtccagcgt cttgttgcag tagcggctat agttgccgcc cccgaagctg
     1981 ttggccgccg aggggatctg gttacaggca aacaaggaag agtcgtcggc gtcgtaggtc
     2041 cacgaatctt caaactcgcc aatgtcaaaa ttgccttgag gcaacaccgt cccgaagtag
     2101 gtgtcggccg aatagttctg gatatccagt ttgatgccaa tggcctgcag gtcttgctgc
     2161 aagatggtct catcggcagc acgccaggca ttgttggcgg tggtcgagta cttgaactcc
     2221 agccgcaagc cgttcttggt gcgcacccca tcggcgccct tggtatagcc ggcttgatcc
     2281 agcaaggcat tggcggcggc cggatcaaac ttggggcagg tgatattggc ttgatagcca
     2341 ggcacataag ctttgccgtg atcggtacac agcggcaccg cctcgcccct tcgcgccgtc
     2401 tggatcaagg cgttgtagtc gatagccatg gagatggctt tgcgcaccgt cacatccttg
     2461 agggcaggtt tatccaggtt gaagtacatg gcctcgaagt tggaggagtt aggattggag
     2521 accaggtgat agttggcgag cgccttgtag gcgggcacct tggtcacatc caggaaccag
     2581 gccgaagtga tcgagcccgc ctggaaatcc ttgaggatgg tgttctgatc gggaatgagg
     2641 cggaaaacga tcttatcgag gtagggcaga ccctccgaag cgcgataata cttgggattg
     2701 cggaccacgg tgaaatggtc gccgggtttg ctctcgctca tcatgaaggg gccgtcggtg
     2761 accgaaggat tgagattgtc gggcgacttc aaaatggcat caggcgacat actgctgaaa
     2821 tggtgggccg gcagcggcgc gaagagtgca tcggcccaga cagtattgaa ggccacaaag
     2881 ggctggctg
//
LOCUS       DATGFG010001026         5455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1311219, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001026 DATGFG010000000
VERSION     DATGFG010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311219"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VKQ52_19500"
     CDS             complement(<1..524)
                     /locus_tag="VKQ52_19500"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HLZ89453.1"
                     /translation="MKKIVVIALLMGAARLCHAQTTVLRDPEIESMIREVSADSLQSY
                     IKTMVSFGTRSTVSSVTDRKKGIGAARNWVLGKFEQWAAGSGGRLTAFVDTTTYAADG
                     RRVKEPINLGNTVATLKGTDPNDNRIFVISGHLDSRRTDVMDGTNDSPGANDDGSGSA
                     AVMECARVMSRHSFP"
     gene            683..3814
                     /locus_tag="VKQ52_19505"
     CDS             683..3814
                     /locus_tag="VKQ52_19505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89454.1"
                     /translation="MRRLLFVAVAILFACAQGMAQTVMTVNGKVTDEKGASIAGATIT
                     VKGTRNATTTKEDGTWSIRVKPKTKLLISYVGYELAEVEAKENLVISLNPDTKALSDV
                     VVTGIGVATSKKKVPIDVATVSSKDFAASTTTNVQQALDGQIAGARIQQTSGTPGAAF
                     NITLRGINSLDGTNPLIMVDGVEMDNLNNIDPSMVDHVEVVKGPAGGMLYGAQGANGV
                     IQIFTKKGALNSRMNISFNSKVSIDNILKGPHPILTTHHHFVTDAGNNILDQSGKPVA
                     RDATGIWTDAQVPTPTTNPFLTNDKTYNLPVYDHLKQGFRQALTFTNSISITGGTASA
                     EYALTASQLNQQDVLSNAYSRSNLSFNLGLHPFKGFTFRTITQGVVGYDNLLNGNRFN
                     MLTAYNYIDFTWKDSTGHYPFKTNASNNGYNTLSENQWHHRHDQTLEIFQNFDFNYKF
                     DRFVELDVKYGLDLNTFDLTNYYQNQSAVLQANQYWGPSRAGSLTDNYHRFFNQNGLY
                     SAFFRTDFEKDFHSRLPIKTTTQVAYDYRKYNMREYFAQGIGLPTYPPANINSASTKT
                     AGDSSLVSTTFGILVNQTIEYGNLFGISAGVRSDYGSAFGAAYQAATFPRGTIFFRPS
                     ELMTAQQSWLRDWKLRAAYGAAGIQPNAYDRQITLTATTLGTGVAVALPSQNTNDSLR
                     LAKNYELEVGTDISFTPFSGSWLSRIDLSGSYWHRESKDVYQNAVVAPSTGYATRLDN
                     LSTIVSHGVDLSLDAVMYNSPGFSWNISARWGSAKAIVTKIAGGQDIVNGGFAIKQGQ
                     VLGLFYVQTPLHSVTQTGADGKTPIIAPANQGNYTVTSTGVVVARSTNYAQYTPTNNL
                     SVAGHAYPDFTSSLINRFILFRTLTFSFQFDWIQGNSIYDITKQWLYRPTGGTGGQGG
                     ASKDLDKKLTIAGQTGSFVNYYNSLYNLGLPSSPFIEKGSYIRLRDLSIGYDFTQFVT
                     NTRVIKRISLTASGRNLLTFTKYSGLDPENTGAYDVQGNDLSHNRTGAFSGVDYFGTP
                     NLRSYQVSLNVGF"
     gene            3838..>5455
                     /locus_tag="VKQ52_19510"
     CDS             3838..>5455
                     /locus_tag="VKQ52_19510"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ89455.1"
                     /translation="MKCQLYRLRSSKQRLLPFLLFVLLLGACKKTILQLPNPDSPTPG
                     ASLITEGGIDAFAQGMFYKWIAYETGDGNLNFFDIAWLMESNMGDEDFSPYSNFGGRY
                     PMNISTITLPAPYNTVVHNPSGFTTSLDILRAENTRTAGDGNSIQYEWDCFYYVNAQA
                     NTLLLALDNPSLQFTGDAATKKKLLQAWAYYWKGYCYSKLGSLYLAAIIDDSPDSTAK
                     GLTSNVYVSHTDVIAAANRNFDQAASLFGSITENADYDATFKAIIPSFNLPNQVITPA
                     MWLHQIHTFEARNYLANHKVATMASSDWTTVLNLATSGMAKNDYTLQWGMSPTGINDL
                     TSFGFFFHPYARHTIAGGALSFVSERLIQDFRPGDQRFVKWFGPYPTGTVVNVLSRGI
                     QFGTRYGVIDIENGGKYASENHLGTVPIAPTWEENALMIAEADIYLGQTDNGAQLIDQ
                     VRAAQGAALPALAGTGISMDSAIAHLHSERRIGLYLHNTSWYDARRWGVTAPAAQGGG
                     RANANVVIPGTLINKTAATVLPCFIDYSFVD"
BASE COUNT         1289 a         1879 c         1258 g         1029 t
ORIGIN      
        1 ggaaaagaat gccggctcat cacccgcgca cactccatga cggccgcact cccactgcca
       61 tcatcattgg cgccggggga gtcgttcgtc ccatccatga catccgtccg ccggctgtcg
      121 agatgaccgc tgatgacaaa aatacggttg tcattggggt ccgtaccctt cagcgtcgcc
      181 acggtgttgc caaggttgat cggttccttt accctcctgc cgtcggccgc ataagtcgtc
      241 gtgtcgacaa aagccgttaa ccggcccccc gagcccgccg cccattgctc gaatttgcca
      301 agcacccagt tgcgcgccgc accgataccc ttcttcctgt ccgtaaccga actgacggta
      361 ctcctcgtac caaaagaaac catcgtcttg atataggatt gcagactgtc cgcactcact
      421 tccctgatca tggactcgat ctcgggatcg cggagaaccg tggtctgcgc gtgacacagg
      481 cgggcagcac ccattaacaa tgcaataaca acaatcttct tcatggtaaa taagcagttt
      541 tgacaaatat cttctttttc tccaactacg tcatttatta gtgagtccct ttgctaaatt
      601 ttagttgcta ctgcagaagc ggatcaccca tcaaccgtta cctttccgta gagccaccat
      661 atcaactaac caaaccaagt ttatgagacg actgctattt gtagccgtgg ctatcctttt
      721 tgcctgcgcg caagggatgg ctcaaacggt catgaccgtc aacggaaaag tcaccgacga
      781 aaaaggcgcc tccatcgccg gcgccaccat cacggtaaaa ggcacccgaa acgccactac
      841 caccaaagaa gacggaacct ggtccatccg cgtaaagccc aaaaccaaac tcttgatcag
      901 ctatgtcggc tacgagctgg ccgaggtcga agcaaaagaa aatcttgtca tctccctgaa
      961 ccccgacaca aaagccctct ccgacgtcgt cgtcacgggc atcggtgtgg ccaccagcaa
     1021 aaagaaggta cccatcgacg tggccaccgt ctcatcaaag gatttcgccg ccagcacgac
     1081 gaccaacgta caacaggccc tggacggaca gatcgccggc gccaggatcc agcaaacaag
     1141 cggaacgccc ggcgccgcgt tcaatatcac gcttcgcggg atcaacagcc tcgacggcac
     1201 caacccgctc atcatggtcg acggagtgga aatggataac ctcaataaca tcgatccctc
     1261 catggtcgac catgtggaag tggtaaaagg cccggcgggc ggcatgctct acggcgccca
     1321 gggcgccaac ggggtcatcc agatctttac aaaaaaaggc gcgctcaaca gccggatgaa
     1381 catcagcttc aattccaagg taagcatcga caacatcctc aaaggccccc atcccatcct
     1441 gaccacgcat caccactttg tcaccgatgc cggcaacaac atcctcgatc agagcgggaa
     1501 gcccgtagcc agggacgcaa ccggcatctg gacggatgca caggtgccca cccctaccac
     1561 caaccccttc ctgacaaatg acaagaccta caacctgccg gtctacgatc acctgaagca
     1621 gggattccgg caggctctca ccttcacgaa ttccatcagt attaccggcg gtaccgccag
     1681 cgccgagtac gccctcacgg cctcccagct caatcaacag gacgtactca gcaatgccta
     1741 cagccgctcc aacctgtcgt tcaacctggg tctccacccc ttcaaagggt tcaccttccg
     1801 caccatcacc cagggtgtcg tcggctatga caacctgctc aatggcaacc gcttcaacat
     1861 gctcacggca tacaactata tcgacttcac ctggaaggat tcgaccggac actacccttt
     1921 caaaacaaat gcttccaaca acggatataa cacgctgtcc gagaaccaat ggcaccatcg
     1981 ccacgatcag acgctggaga tcttccagaa cttcgacttc aactataagt ttgatcgctt
     2041 cgtcgaactg gacgttaaat acggcctcga tctaaacacc ttcgacctca ccaactacta
     2101 ccagaaccaa agcgctgtgc tccaggccaa ccaatattgg gggcccagcc gcgcagggtc
     2161 gctcaccgat aactatcacc gtttcttcaa ccagaacggc ctgtattcgg cgttcttccg
     2221 cacagacttc gaaaaggatt ttcactcccg cctgcccatc aaaaccacca cacaggtggc
     2281 ctatgattat cgcaagtaca acatgcggga atattttgcc cagggcatcg gcctgcccac
     2341 ctatccgccg gccaacatca actccgcctc taccaaaacc gccggtgact cctccctcgt
     2401 gtctaccacc ttcgggatcc tggtcaacca gaccatcgaa tatgggaatc tcttcggcat
     2461 ctcggcaggg gtccgctccg actacggcag cgccttcggc gcagcctacc aggccgctac
     2521 cttcccgcgc ggaaccatct tcttccggcc ttccgagctg atgacggcgc aacagtcctg
     2581 gctgcgtgac tggaagctcc gggccgccta cggcgccgca ggtatccagc ccaacgccta
     2641 cgaccgccag atcacgctca ccgctaccac cctgggcacc ggcgtagccg tggcacttcc
     2701 ctcgcagaac acaaacgata gcctccgcct cgccaagaat tacgagctcg aggtcggtac
     2761 cgacatcagc ttcacgccct tcagcggcag ctggctctcc cgcatcgatc tcagcggcag
     2821 ctactggcat cgcgaatcca aagacgttta tcaaaatgcg gtcgtggcgc cctccaccgg
     2881 ttacgccacc cggctcgaca acctgagtac catcgtctcc cacggcgtgg acctctccct
     2941 cgacgcggtg atgtacaaca gcccgggctt cagctggaat atcagcgccc gctggggatc
     3001 cgccaaggcc atcgtgacaa agatcgccgg cggtcaggat atcgtcaacg ggggcttcgc
     3061 catcaagcag ggccaggttc tcggcctgtt ctacgtacag acacccctgc actcggtaac
     3121 ccaaacgggc gccgatggca aaacacccat tatcgccccc gccaaccagg gcaattacac
     3181 ggtcaccagc accggtgtag tagtcgccag gtccaccaac tatgcccaat acacgccgac
     3241 gaacaatctg tccgtcgctg gacatgccta cccggacttc acctcctccc tcatcaaccg
     3301 cttcatcctg ttccggacac tgaccttctc cttccagttc gactggatac aagggaacag
     3361 tatctacgac atcacaaaac agtggttgta ccgccccaca ggcggaacag gcggccaggg
     3421 cggagcatcc aaagatctcg acaagaagct gaccatcgca gggcagacgg gctccttcgt
     3481 caactattat aatagcctct acaatctcgg cctgcccagc tcccccttca tcgagaaggg
     3541 ctcctacatc cggctccgcg atctgtccat cggctatgac ttcacccagt tcgtaaccaa
     3601 caccagggtc atcaagcgga tcagcctcac ggcatcgggc cgcaacctgc tcacgttcac
     3661 aaaatacagt ggactggacc ccgagaacac aggcgcctac gacgtccagg gcaacgacct
     3721 ctcccataac aggacaggcg cattctccgg tgtcgactat ttcggtacgc ccaacctccg
     3781 gtcttatcag gtctccctca acgtagggtt ctaatcgatc atcttaatct tactaaaatg
     3841 aaatgtcaac tatatcgact ccggtcttca aagcaacgcc tcctgccctt cctcctgttc
     3901 gtactgctgc tgggcgcatg caaaaaaacg atccttcagc tgccgaaccc ggactctccg
     3961 acccccggcg cttccctcat caccgaaggc ggcatcgacg ccttcgcgca aggcatgttc
     4021 tataaatgga tcgcctacga aaccggcgac ggcaacctca atttcttcga catcgcctgg
     4081 ctcatggaaa gcaatatggg cgacgaagac ttttcaccct attccaactt cggcggccgt
     4141 tatccgatga acatcagcac catcacgctc ccggcgccct acaacacggt cgtccataat
     4201 ccctccggct ttactacctc gctggatatc ctccgggccg agaatacccg cacggccggc
     4261 gacggcaact ccatccaata cgagtgggac tgcttttact atgtcaacgc acaggcaaac
     4321 acgctgctcc tggcgctgga taatcccagc cttcagttca ccggcgacgc cgccacgaaa
     4381 aagaagctgc tccaggcctg ggcctattac tggaaagggt attgctattc caaactgggc
     4441 tcgctctacc tggcagccat catcgacgac tcccccgaca gcaccgcgaa aggactcacc
     4501 agcaatgtat acgtaagtca taccgacgtc atcgccgcgg ccaatcggaa tttcgatcag
     4561 gccgcttccc tgtttggcag catcaccgag aacgcggact atgatgccac cttcaaggcg
     4621 atcatcccca gcttcaacct gcccaaccag gtcatcacac cggccatgtg gttacaccag
     4681 atccatactt tcgaggcccg taactacctg gcgaatcaca aagtggccac gatggcgtcc
     4741 tccgactgga cgacggtcct caacctcgcc acctccggca tggccaaaaa cgactatacc
     4801 ctgcagtggg gcatgtcgcc gaccggcatc aacgacctca cttccttcgg gttcttcttc
     4861 catccttacg cccgccatac catcgccggc ggagcactct ccttcgtcag cgaacggctg
     4921 atccaggact tccggccggg cgaccagcgc ttcgtgaaat ggttcggacc ctaccccacc
     4981 ggaacggtcg tcaacgtgct tagccggggc atccagttcg gcacacgcta cggcgtcatc
     5041 gatatcgaga acggcgggaa atatgcctcc gagaaccatc tcggaacagt acccatcgca
     5101 cccacctggg aagagaatgc actcatgatc gccgaagcgg atatctatct cggtcagacc
     5161 gacaatggcg cccaactcat cgaccaggtc cgggccgccc agggcgcagc cctgccggcc
     5221 ctcgccggca caggcatttc catggactcg gccatcgccc acctgcacag cgaacgacgt
     5281 atcggcctct atctccacaa cacatcctgg tacgatgccc gccgctgggg cgtcacggcg
     5341 cccgccgccc agggcggcgg aagagccaac gcaaacgtag tgatcccggg caccctgatc
     5401 aacaaaaccg cagccaccgt gctgccctgc ttcatcgact acagcttcgt cgatt
//
LOCUS       DATGFG010001027         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1624696, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001027 DATGFG010000000
VERSION     DATGFG010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624696"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VKQ52_19515"
     CDS             <1..716
                     /locus_tag="VKQ52_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89456.1"
                     /translation="INFDLVYNGNYYDAAYDITLTAIVTYQLIDVSPWVTIMDKFVTP
                     TETFTAEIPPSLSQLLDLLTALKSQVDAAQNNGNLYGNTVFGNLVTAYNYAMSIYTHL
                     IDRGSVGKLAGLSSYVYQLQTILNNGVTPTYVNTNTPIPAYSWGGGSGGTTWGSITGK
                     PTTGKIEWTVGQGGFPAAGANSITSTKLAGVPIAQIMMFRGGIYYPAISKSSDASNQL
                     NWTDSLANQEPILILIIAL"
     gene            722..2359
                     /locus_tag="VKQ52_19520"
     CDS             722..2359
                     /locus_tag="VKQ52_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89457.1"
                     /translation="MKRIIFLLGLFASALTGFAQAPLGCPTCTTEVKGILKADSLFLL
                     PLRNDTLVNPGRPGALVNAYGQTWLYTGTQWAKLTGVAWGSIPGNITAQTDLMALLNA
                     KQNQISNGYGWKLTGAVGSIDTAVLRKVDSVWKLNDSTIQYTINGRPPTSIVIKGSAV
                     GTIVTAISFSAPSGIFNTPVTLANSSGSWTGTLSLITQGANTIFAGPATGSAAAPGFR
                     PLVTADLPTSIPNSNLANNFIGFGIDNTGSAPGWSSTSPPLGSATVLHLPVVGPSTTG
                     IVNPPLFAYWNAKIDSTTMSNDSVYEWRNGIRYSRYMIAAGSGLLSLNGQTGSTQSFQ
                     NGTAGTAPNISSAGNVHTFNNPLVNGADTGIVTPGLYNTWNAKEPAIAAPNTTNRYWN
                     GYKNFVPLNTDSIAQGTSNKFFVTGDTAGLSGKEPGITAPNTTNKYWNGYKQFVPLNT
                     DSIAQGTTNKFFVTGDTAGLSAKMTNFGGAPGQLEGTFAGIPAATLYATGTTYIAQDS
                     GFQYVDTGSGGSRGWKKISGGSGGSGGSGSVNTFRAN"
     gene            2588..>3323
                     /locus_tag="VKQ52_19525"
     CDS             2588..>3323
                     /locus_tag="VKQ52_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89458.1"
                     /translation="MGASTGIKAGAFLSRPTSTNSQSYYWSTDSVFWSYDGPTGWKDL
                     KPGSGGGGTVTTDTTLVGNGSGTPLGLNSNLVTSANYYNRPYPLSNSSLGFGTSIYAG
                     YGITAGLAGAPMALVQSYLNNTVVNNGAVASTAVRTAYNYISANMPQYFFSTLSGESG
                     FNNIRTPISGDTLHRFGHVKAAYRALAALLFSNQSNIQFYFIGTGTLNPNITTSLTCS
                     GCTAPAIDTLKDFSSRTVYYRANVPGF"
BASE COUNT          825 a          852 c          855 g          791 t
ORIGIN      
        1 ggataaattt tgacctcgta tacaacggca attattacga tgccgcgtat gatataaccc
       61 tcaccgccat cgtgacgtac cagttgatcg acgtcagccc atgggtgacg attatggaca
      121 agttcgtaac gccgaccgaa acattcacgg cggagattcc gccgagtctt tctcagttgt
      181 tggatcttct cacggccctg aaatcacagg tggacgccgc ccaaaacaat ggcaacctgt
      241 atggcaatac cgtcttcggt aacctcgtca cggcgtacaa ctatgccatg tcgatataca
      301 cgcaccttat cgaccggggt tcggttggca aactggcggg gctttcttcc tacgtctacc
      361 aattgcagac gatcctcaac aacggagtta ctccgaccta tgtgaacact aacactccaa
      421 ttccggccta tagttggggc gggggaagcg gcggtacgac gtggggcagt attaccggca
      481 aaccaaccac tggaaagatt gaatggactg tcggccaggg tggatttccg gccgccggcg
      541 caaactcaat tacgtctaca aaattggcgg gtgtgccgat cgctcaaatt atgatgttcc
      601 ggggaggaat ttactatccg gctatttcga agtcgtcgga tgcgtccaat cagttgaact
      661 ggacagattc tctggccaac caggaaccta tcctcatttt aataatcgca ctctgataat
      721 catgaaacgt attatattct tactgggtct ttttgcatct gcgctgactg gcttcgctca
      781 ggctcctttg ggatgtccaa cctgtactac tgaagtaaag ggtatattga aagctgacag
      841 tctcttcctg ctgccgttgc gaaacgatac cctggtcaac ccgggccgac ctggtgcact
      901 ggtgaatgca tatgggcaaa catggctgta taccgggact cagtgggcga agctgacggg
      961 cgtggcatgg ggatcgatac cgggcaacat tacggcgcag acggacctta tggcgctgct
     1021 caatgccaag cagaaccaga tatcgaatgg ctatggatgg aaattgaccg gggccgtagg
     1081 tagtatagat actgctgtct tgcgcaaggt ggattcagta tggaagctaa acgactctac
     1141 catccagtat acgatcaatg gcagacctcc gacgagtatc gtgatcaagg ggtcggctgt
     1201 cgggacgatc gtgacagcga tttcctttag tgccccctcc ggtattttca atacacccgt
     1261 cacgctggct aattctagtg gctcgtggac cggtacattg tcgctgatca cgcaaggagc
     1321 gaacaccatc ttcgctggtc ccgcaacggg atcggcggcg gcgccgggat tccggccgct
     1381 cgtcactgct gacctgccaa cgagcattcc aaactccaat ttagcgaaca actttatagg
     1441 ctttggcatc gacaacaccg gatcggctcc tggctggtcg agcacttcgc caccactggg
     1501 ttcggcaact gtcttacatc tcccggtagt ggggccgtcc actactggca ttgtcaaccc
     1561 accactattc gcctattgga atgcaaagat cgacagcacg acgatgagca acgactctgt
     1621 atatgaatgg cggaacggta tccgatattc cagatatatg atcgctgccg gtagcgggtt
     1681 gctaagcctg aatgggcaga cgggaagtac acagtcattt cagaatggaa cggcgggtac
     1741 cgcaccgaat atatcaagcg caggcaatgt gcatacgttc aacaatccgc ttgttaacgg
     1801 cgccgacacc ggtattgtga cacccggact atataataca tggaacgcaa aagagccggc
     1861 tattgcagca cctaacacaa cgaataggta ttggaatggt tacaaaaatt ttgttcccct
     1921 gaatacggac tcaatagccc agggtaccag caacaagttt tttgtcaccg gtgacacagc
     1981 tgggctatcg ggcaaggagc ccggaatcac ggcgcccaat acgaccaata agtattggaa
     2041 tggttataag caattcgtgc cattgaacac cgactctatt gctcagggaa cgacaaacaa
     2101 attctttgtc acgggagata ctgcgggact atcggccaag atgacgaatt tcggcggagc
     2161 tcccggtcag ttggagggta cttttgccgg tatacctgct gccactttgt acgccaccgg
     2221 tacgacctat attgcgcagg acagcggatt tcaatacgtc gatactggat cgggtggttc
     2281 gagaggatgg aagaagatca gcggcggatc gggaggctca gggggatctg gatcagtgaa
     2341 tacttttcgc gcgaattgac agccagggga tacctcgtac gtttccggaa atgggacgaa
     2401 taatattatc aaggtcgccg cacgtcggga cagcttggac catcacacag taatcaatcc
     2461 cgatagctcg aggacttttt atgttcaagc gcatgattcg acatcggcta atgcgttaca
     2521 gacgaaatat cggtcggata ctagcagaac aaatatctat accgcattag cccaaaaggt
     2581 cactaacttg ggagcctcta caggaataaa ggccggagcc tttttatcgc gtccaacttc
     2641 caccaattca cagtcatatt attggtccac cgactcggtt ttttggtctt acgacggtcc
     2701 caccggctgg aaggatttga agccgggaag cggaggaggg gggactgtga ccacagatac
     2761 aacactagtc ggaaacggct ccgggacacc tttgggactc aattccaacc tggtcacttc
     2821 ggcgaattac tataaccgac cctacccgct ttcgaattct tcgcttggtt ttggtacttc
     2881 gatctatgct ggttatggta tcaccgcggg attggcgggg gcgccgatgg ctcttgtgca
     2941 aagttatctg aacaatacgg tggtcaacaa cggggccgtg gcctctactg ctgttcgcac
     3001 cgcctacaac tacatttctg ccaacatgcc gcaatatttt ttcagcacac tgtctggcga
     3061 atcagggttt aacaatatcc ggactcctat ttcgggcgat acactccata ggtttggtca
     3121 tgtaaaagct gcctatagag cgctggcagc gttattgttt tctaaccagt cgaatattca
     3181 attttacttc atcggcacgg gtactctcaa tcccaatatc accactagcc ttacctgtag
     3241 cggatgtacg gcgccggcca ttgatacact gaaggacttc agctccagaa cagtgtatta
     3301 tcgggctaat gtaccgggct tta
//
LOCUS       DATGFG010001028         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_400267, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001028 DATGFG010000000
VERSION     DATGFG010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400267"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VKQ52_19530"
     CDS             complement(<1..524)
                     /locus_tag="VKQ52_19530"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLZ89459.1"
                     /translation="MVHNFNAGPSILPKEVFEQASRAVLDYNGTGLSILEIGHRTPLF
                     QEVVDEAMATARELMKLDSDHEVLFLHGGASTQFFQVPMNLLNEQDVAAYLDCGVWGK
                     KAIKEARLFGKVEVVASSKDRNYTYIPKGYEIPADAAYFHYTTNNTIEGTQMHAIPET
                     SVPLVADMSSDILS"
     gene            complement(557..1183)
                     /locus_tag="VKQ52_19535"
     CDS             complement(557..1183)
                     /locus_tag="VKQ52_19535"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HLZ89460.1"
                     /translation="MPARQIPRKLFRLFLQGLLILAPISITIWILVTAFYWIDGILPN
                     LVYSISPKLIGTDSVGLPRRIPGLGFIVFIAIALIVGYISPSWIVSRIVEFAESVLEK
                     TPGIKLIYSTIKDFFEAFAGNKRKFTKAVLVSVGAPDVWQVGFITQEELHEFDLQEYV
                     AVYLPQSYAFAGHLYFVKRDRVRVLTDISSADAMKFAISGGVAEVDEQ"
     gene            1391..2038
                     /locus_tag="VKQ52_19540"
     CDS             1391..2038
                     /locus_tag="VKQ52_19540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ89461.1"
                     /translation="MEVRTDVKERILTKAAELFMRYGIRSITMDEIAAQLGISKKTIY
                     QFFTDKDDMVFAVIEQEIGKNEAECLQYRYDSADPVHEIFLAVEDLEEMLRYTNPMML
                     YDLEKYHPRAFQKIREYKYQFLFDAILENLRKGIEEGVYRSDIQRDIVAKNRIESAFL
                     VFNPDLFPHSRYKMSEVNFELAMLFLYGIVTDKGRKLIEKYTEERTKKLSHEQNA"
     gene            2022..3353
                     /locus_tag="VKQ52_19545"
     CDS             2022..3353
                     /locus_tag="VKQ52_19545"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ89462.1"
                     /translation="MNKTLSRLLGIPFLLVVLNLRAQQVHDFSLPQAIDYASKNSVLV
                     KNALLDYELQVQSNRATTSQALPQVTGNLGITDNLQIATVLVPGTFASPPVQGFIPLK
                     FGTQYNNTAGVTLKQILFDGQVFVGLQARQTSLNFYKKKQEVTEQLVRANIYKIYYQL
                     LIGKWQIDQIDANIASQQALLHNSTEMFKNGFAEQLDIDKATVQLTNLESERIQVDYS
                     ISNGYLGLKVLMGMSVKDSLHLTDTLTYEMVRDATLGDNYKYSDRRDFQLLQINRQLN
                     EFDIKRYRKMYIPTVSLTGNYSQNQYDNQFNLGKKNSWFPSSYLGLNVSVPLFDGFYK
                     AANIQQARLRLEQTQNDLDSLKNRIDNDVKETQLRFTAALATLDFQKKNLELSEKVYA
                     QTRKKYEQGLGSNLEITTAFADERTAQANYFNALYNAIAARVDYLNAIGKL"
     gene            3381..>4133
                     /locus_tag="VKQ52_19550"
     CDS             3381..>4133
                     /locus_tag="VKQ52_19550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ89463.1"
                     /translation="MHKILTVVVASSLLLAACGSGSVQKDDSLQGKKARLAALKDQQE
                     SLSKQIASLEDDIAKSDSTAGTKEKAKLVALTALTPTSFIHYIDLQGNVDAENYSNIA
                     PQGNGGIVREVFVKQGDHVNKGQLLLRLDDAVQRQQLTNAETQLAYAKDIYNRRKNLW
                     DQKIGTEVDLINAKNQVDQAGTQLKIYQEQLDWTNVYADIPGTVNTLTIKVGEAFLSA
                     VQSQSPQLQIINTDQLKAVVQVPEIYQERIHVG"
BASE COUNT         1143 a         1196 c          948 g          846 t
ORIGIN      
        1 ctgctcagga tatcactgct catatcggcc accagcggaa cggaggtctc cgggatggca
       61 tgcatctgcg tgccctcgat cgtattgttc gtcgtataat ggaaataagc ggcatcggcg
      121 gggatctcgt accccttcgg aatataagtg tagttccggt ccttcgaact ggcgacgact
      181 tctaccttcc caaataacct tgcttccttg atggccttct taccccagac gccacagtcg
      241 agataagccg ccacatcctg ctcgttcagc aggttcatcg gtacctggaa gaactgggtg
      301 gacgccccgc catgcaaaaa aaggacctca tgatcactgt ccagcttcat cagctcccgc
      361 gcagtcgcca tcgcctcgtc aaccacctcc tggaacaaag gagtccggtg gccgatctcc
      421 aggatcgaca accccgtacc attataatcc agcacggctc ggctggcctg ctcaaaaact
      481 tccttgggta agatactggg accggcattg aaattatgaa ccacgattcg aatttttttt
      541 gaaggtcaaa gataagctat tgctcgtcaa cttctgcgac cccacccgag atggcgaact
      601 tcatcgcatc ggcggaactg atatccgtca gcacccgcac cctgtcccgc ttgacaaaat
      661 ataaatgacc cgcaaaagca taggactgcg gcagatatac cgcaacatat tcctggagat
      721 cgaactcatg cagttcctcc tgggtgataa aacccacctg ccacacatcg ggcgcgccga
      781 cactcaccag cacggccttc gtgaacttcc gcttgttacc ggcaaaggcc tcgaagaaat
      841 ccttgatcgt cgaatagatc agcttgatac ccggagtctt ctccagcacg gactcggcaa
      901 attccacaat tcgactcacg atccatgaag gcgaaatata ccctacgatc agcgcgatag
      961 cgataaagac gatgaatcca agtccgggta tcctcctcgg cagcccgacg gagtccgtcc
     1021 cgatcagctt cggagagatc gaatacacaa gattcggcag gatgccgtcg atccaataaa
     1081 aagcggtgac cagtatccag atggtgatcg aaatgggagc gaggatcagc agcccctgca
     1141 gaaaaagccg gaatagcttg cggggaattt ggcgtgcggg catgggcagt ttttagagca
     1201 taaaagtact catttctacc ccgatccccg ctttatggcc cgtcaataac cccatacagc
     1261 ccgttaattt accattcacc gatgaaatcg gccattcgcc ggcaaataac ccactatccg
     1321 ccatcggaaa ctttggtaac attaaaagtt tccctagttt tgcccctgca aaagcacagg
     1381 agaacaagaa atggaagtaa gaacagatgt aaaggaaagg atcctgacca aagccgctga
     1441 actatttatg cggtacggca tccgctccat caccatggac gagatcgccg cacagctggg
     1501 catttcgaaa aagactatct atcaattctt tacagacaaa gacgacatgg tattcgccgt
     1561 catagaacag gagatcggca aaaatgaggc cgaatgtctc caataccgct atgactccgc
     1621 cgaccccgtc cacgagatct tcctggccgt ggaagacctg gaagaaatgc tccggtatac
     1681 caaccccatg atgctctacg atctggaaaa gtaccacccc cgcgccttcc agaagatcag
     1741 ggaatataaa taccagttcc tcttcgacgc catcctcgaa aaccttcgca aaggcatcga
     1801 agaaggcgtg tatcgtagtg atatccaacg tgatatcgtt gccaagaacc gtatcgagtc
     1861 cgccttcctg gtcttcaacc ccgacctctt cccccatagc cggtataaga tgtcggaagt
     1921 caatttcgaa ctcgcgatgc tcttcctcta tggcatcgtc accgataaag gcagaaagct
     1981 gatcgaaaaa tacaccgaag aacgcaccaa aaaactatct catgaacaaa acgcttagcc
     2041 gacttttagg gatccccttt ttgctggtgg ttttgaacct ccgtgcccag caagtgcatg
     2101 atttctccct tccacaggcg atcgactatg catcgaagaa cagcgtgctg gtaaagaacg
     2161 ccctgctgga ttacgagttg caggtacaaa gcaaccgcgc cacgacctcc caggccctgc
     2221 cgcaagtgac cggcaacctg ggaattaccg ataatctcca gatcgctacc gttctggtgc
     2281 cgggcacttt tgccagccct cccgtacagg gctttattcc cctcaagttt ggcacacaat
     2341 ataataatac ggcgggggta acattaaagc agatcttgtt cgacggacag gtatttgttg
     2401 gcctgcaggc gcgccagacc tccctgaact tctacaagaa gaaacaggag gtcactgaac
     2461 aactggtacg cgccaatatc tataagatct actaccagct gctcatcggc aaatggcaga
     2521 tcgaccagat agatgccaac atcgccagcc agcaggccct gttgcataat tcaacggaga
     2581 tgtttaagaa tggctttgcc gaacaactgg acatagacaa agctaccgtc caactgacca
     2641 acctcgagtc ggaaaggatc caggtggatt acagtatctc caacggctac ctcggactca
     2701 aggtcctcat gggcatgtcg gtgaaggact ccttacatct caccgacacc ctcacttacg
     2761 agatggtccg ggatgctacg ttgggagata attacaagta tagtgatcgc cgggatttcc
     2821 agttgctaca gatcaacagg caattgaatg agtttgatat caagcggtat agaaaaatgt
     2881 atattcccac ggtctcgctg acaggcaatt attcgcagaa ccaatacgat aaccaattca
     2941 acctggggaa aaaaaatagc tggttccctt cttcctattt gggtctgaac gtttcggtgc
     3001 cccttttcga tggtttttac aaagccgcga atattcaaca ggccagactc cggctggaac
     3061 agacgcagaa cgatttggac tcactgaaga accgcatcga caacgacgtg aaggaaacac
     3121 agctccggtt cactgctgcc ctcgctaccc tggatttcca gaaaaagaat ctcgaactct
     3181 cggaaaaggt atatgcccaa acccggaaaa aatacgaaca gggcctcggc tccaacctcg
     3241 aaataacaac ggcctttgcc gacgaacgga cagcacaggc caattatttc aacgccctgt
     3301 ataacgccat cgccgcccgg gtggattacc tcaacgccat cggaaaactc taaccaacca
     3361 aacattaaaa atcgatagat atgcataaga tactgactgt tgtcgtcgcc tcttccctgt
     3421 tgctggctgc ctgcggcagc ggctccgttc agaaggacga ttccttgcaa gggaaaaagg
     3481 cccggctcgc cgccctgaaa gaccaacagg aaagcctgtc caaacagatc gccagcctcg
     3541 aggacgatat cgctaaatcc gattccaccg ccggaacaaa ggaaaaagcg aagctcgtgg
     3601 cgctgacagc cctgaccccg acatccttca tccactatat cgacctccag ggtaacgtcg
     3661 acgcggagaa ctactccaac atcgcacccc agggcaacgg cggtatcgtc cgggaagtct
     3721 ttgtcaaaca gggcgatcac gtcaacaagg ggcaactgct cctccgcctc gacgacgccg
     3781 tacagcgcca gcagctcaca aatgccgaaa cccagttggc ctatgcaaaa gacatctaca
     3841 atcgcagaaa gaacctctgg gatcaaaaga tcggcacgga ggttgacctc atcaacgcaa
     3901 agaaccaggt cgaccaggcc gggacccagc taaagatcta ccaggaacag ctggattgga
     3961 caaacgtcta tgccgatatc cccggtacgg taaatacgct taccataaag gtcggcgaag
     4021 ccttcttatc cgccgtccag tcccagtccc cccaactcca gatcatcaac accgaccagc
     4081 tgaaagcggt cgtacaggtg cccgagatct accaggaacg catccacgtc ggc
//
LOCUS       DATGFG010001029         5730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_287961, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001029 DATGFG010000000
VERSION     DATGFG010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287961"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VKQ52_19555"
     CDS             <1..337
                     /locus_tag="VKQ52_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89464.1"
                     /translation="VYPEGQFVLLYVRVSNRAQKDRKRPAGLRVEVLDRTGRHWEYSL
                     RGQRALEESSGSQTPLDSYLDAHSSFETELVFDVPKDAKGLRARVDMGPGLLRSLLLP
                     REREVVVLP"
     gene            complement(346..1293)
                     /locus_tag="VKQ52_19560"
     CDS             complement(346..1293)
                     /locus_tag="VKQ52_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89465.1"
                     /translation="MKRHPLTALLLAATLFLATRTAAQDTSSSSKPSIQLGESQWKAV
                     VGVFQSPQNADMKVRFSFRENKLLAELLWNGGALHLQPESATAFTSIEKDDGEAIHIT
                     FRNDSAGRYTTVDVAKNGTWTRVNDYKPAPAKQAMTHTPDQLKRFEGVYHMPDREYMF
                     ISLLIKDNNLVLKQHWDNSELSFQPDTDSTFFMVDRPVFTLDFKKDAGGNITGFTAFK
                     RDNWKRTTKPTLTTAQLAALAGKYQSNDDPDNLIELSARDNHLIVKQLWDKKQITLDA
                     LAEGYFYNGTESYSLQQTKDRDGKPAILLLGMDFFERVK"
     gene            complement(1305..1907)
                     /locus_tag="VKQ52_19565"
     CDS             complement(1305..1907)
                     /locus_tag="VKQ52_19565"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HLZ89466.1"
                     /translation="METNRLKRKTAMIVRWLHIYLSMISFAVVFFFAVTGLTLNHAEK
                     FGDETQTHQEKGTIDPGWINNKDTTKIRRLDIVEHLRHSNNIKGECSDFRIDDDQVTV
                     SFKGPGYAADAFINRATGGYDLTVTTAGLVGVINDLHKGRDTGHGWSVFIDISALFLT
                     LVSLSGLLLLLFLKKRRLSGLLVAAGGLFIAWLVYMIWIK"
     gene            complement(1897..3498)
                     /locus_tag="VKQ52_19570"
     CDS             complement(1897..3498)
                     /locus_tag="VKQ52_19570"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HLZ89467.1"
                     /translation="MKRIPLLLSVILLPTLAAILLPIPAAWATPPPAKTRFFVSHYEN
                     VLGTSMELKVAVPDQQTSDKAEHAVLAEIDRLSKILSGYDPNSEFSRWQKTTGTPVQI
                     SPELFEVLSLFDQWRQRTGGALDASAETITRVWKQAAKEQRLPATTELEAAVEKVRQP
                     HWRLDATTRSAIHLDNAPLMLNSFAKSYIIQHAADAGRAASGADAIIVNIGGDLVVSG
                     HTKETILVSDPTADAENDNPIDRLRVGNAAIATSGNYRRGENIGGHWYSHIVDPRTGQ
                     PADQILSATVVAPGATDAGALATSLNVMSPAESIQLARQVPGVEYLIITRSGERIQSP
                     GWKALQLPATDTRPAANTPATDNTRPSAKTSPTAISPATPLTPSIGKDFELAVNLEIN
                     LQNQGSVKRPYVAVWVEDENHAPVRTITLWHGPDRYLPELKSWFLKYRGLYSADRNFS
                     ASVTSATRSAGKYTVKWDGRDDKGNDVPPGKYIVKIEISREHGTYQLMRQEIDCDDTP
                     KSFTLPGNIEISAASLDYRKKTNGN"
     gene            3683..4135
                     /locus_tag="VKQ52_19575"
     CDS             3683..4135
                     /locus_tag="VKQ52_19575"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HLZ89468.1"
                     /translation="MNAFRKRKEELAGEGKTDGEKSGKSVDGGGVRPLTKAEEQVMKV
                     VWQLGNGLLMEIVESMPKPAPHKNTVATILKTLVDKGFVRVENIGRIHRYHPLVSKEA
                     YSRDTLSSVAKGYFEGSFTNVVSFLVDEKKLSVKDLELLLQELKKNKK"
     gene            4137..5237
                     /locus_tag="VKQ52_19580"
     CDS             4137..5237
                     /locus_tag="VKQ52_19580"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HLZ89469.1"
                     /translation="MERLFTFLFQSVLISGMLTAYYWVVLRNRKLHGYNRWFLLGTVG
                     LSLLLPLVRIRWSPFAFAGEAALPVAAGRAQAAGNAAGGSFPWMAVAGWTGIGVSLVL
                     LGMLLLRVVQVYRLKSRLSYSRMGGYDLIETDDPRAPFSFLNNLFWRRGADPEDPVNR
                     RILYHELAHIGGRHSWDGLFVQSVSCVFWMNPFFWLIRRELSVVHEFIADAATGMEGD
                     TEGFARMLLQSMDAGRFLEPAQGFFQSPIKRRLFMMSNNRRSRFCLLRKALVLPVVMV
                     VTVFVSCTKERAPLVADRVDEIKLNKQKKDEKMMKFMIVGKGSYNVKLSRPLVFVRGS
                     GTDEIKIKQATELMLQKLEMDEKLMKIRFDQK"
     gene            5234..>5730
                     /locus_tag="VKQ52_19585"
     CDS             5234..>5730
                     /locus_tag="VKQ52_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89470.1"
                     /translation="MRREILASMFVGAAVFANAQGIYNGSATRINDLVHTRLEVRFDY
                     AHSYVYGKVELTLRPHFYPTDSLMLDAKQMSISAVALVEPDSGRGSGGSVDAFGRALR
                     KGSGQVSARQLGNGSGQALGRVLARPLGFDYDGWRLRIRLPRQYRRTEQYVVRVVYIA
                     KPAEA"
BASE COUNT         1115 a         1266 c         1939 g         1410 t
ORIGIN      
        1 ggtgtacccg gaagggcagt ttgtgctatt gtatgtccgg gtgtcgaatc gtgcgcagaa
       61 ggataggaag cggccggcgg gattgcgggt tgaggtgctg gatcggaccg ggaggcactg
      121 ggagtattcc ctgcggggac agcgggcgct ggaggagtcg tcggggtcgc agacgccgct
      181 ggacagttac cttgatgcgc attcctcctt cgagacggag ttggtttttg atgtaccgaa
      241 ggacgcaaag ggattgcgag ccagggtcga tatgggccca gggctgctgc ggagtttgtt
      301 gttaccgcgg gaaagggagg ttgtggtgtt gccgtagtat cttttttatt ttactctttc
      361 gaagaaatcc attcccagca ggaggatggc gggtttgccg tccctgtcct ttgtttgttg
      421 cagggaatag gattctgtgc cattatagaa atacccttcg gcgagggcgt cgagcgtgat
      481 ctgcttcttg tcccagagtt gttttacgat gaggtggttg tcgcgggcgg agagttcgat
      541 caggttgtcg gggtcgtcat tggactggta tttgccggcg agggccgcga gttgtgcggt
      601 ggtgagcgtt ggttttgtcg ttcttttcca gttatcgcgt ttgaaagcgg tgaatcccgt
      661 gatgttgccg ccggcgtcct ttttaaaatc tagggtaaat acgggccggt cgaccatgaa
      721 gaaggtggaa tcggtgtcgg gctgaaagga cagttcgctg ttatcccagt gttgtttgag
      781 gacaaggtta ttgtctttga tgagcagcga gatgaacatg tattccctat ccggcatgtg
      841 gtaaacgcct tcgaagcgct ttagttgatc cggggtgtgg gtcatggcct gtttggccgg
      901 cgcgggttta tagtcgttca cacgtgtcca ggtgccgttt ttagccacat cgaccgtggt
      961 gtagcggccg gcggagtcat tacggaaagt gatgtggatg gcttcgccat cgtccttttc
     1021 gatgctggtg aaggcggtgg cggattcggg ctgcagatgc agggcgcccc cgttccagag
     1081 caactcggcc aggagtttgt tctcgcggaa agagaaccgg accttcatat cggcgttttg
     1141 cggggactgg aagactccta ccacggcctt ccattgactt tcgcccagtt ggatggatgg
     1201 ttttgaggag gaagatgtat cctgcgccgc ggtccgggtt gcgagaaaca gggttgcggc
     1261 gaggagcagg gcagtaagcg gatgtctttt catatgtata aagtttattt gatccagatc
     1321 atatagacaa gccaggcgat gaacaggccg cccgcggcga cgagcaggcc gcttagccgg
     1381 cgcttcttca aaaagagcag gagcagcagg ccgctcaggg aaacgagggt aaggaagagg
     1441 gcggagatgt cgatgaacac cgaccagccg tggccggtat cgcggccctt atgcagatcg
     1501 tttatcactc ctacgaggcc ggccgtggtg acggtgagat catatccgcc ggtggcccgg
     1561 tttatgaagg catcggctgc gtagccgggg cctttgaagg aaacggttac ctggtcatcg
     1621 tcgatccgaa aatcgctgca ttcccctttt atattgttgc tgtgccggag atgctctacg
     1681 atgtcgaggc ggcggatctt cgtggtatcc ttattgttga tccagccggg gtcgatggtc
     1741 cctttttcct ggtgggtttg tgtctcgtcg ccgaattttt ccgcgtggtt cagtgtcagt
     1801 cctgtaacgg cgaagaagaa gacaaccgcg aagctgatca tcgacaaata gatgtgcagc
     1861 cagcggacga tcatggccgt ctttcgtttc agccggttag tttccattcg tttttttccg
     1921 atagtcaagg gatgccgcgg atatttcgat atttccgggg agggtgaagg atttgggagt
     1981 atcgtcacag tctatctcct gtcgcatgag ctggtaggtg ccgtgctcgc gggatatctc
     2041 gatcttgacg atatacttac ccgggggtac gtcattgccc ttgtcatccc tgccatccca
     2101 ttttacggtg tatttgccgg cggaacgggt ggcgctggtg accgaggcgc tgaaattcct
     2161 gtcggcgcta tagaggccgc ggtatttgag gaaccaggat ttcagttcgg gcagatagcg
     2221 atccgggcca tgccagaggg tgatggtacg aaccggggca tggttctcgt cttccaccca
     2281 gaccgcgaca taggggcgtt tcaccgagcc ctggttctgg aggttgatct cgagattgac
     2341 cgcgagttcg aagtctttgc cgatggatgg ggtgagaggg gtggcgggtg agattgcggt
     2401 gggtgaggtt tttgccgacg gccgggtgtt gtccgtggcc ggggtgtttg ctgcgggtcg
     2461 ggtgtcggtg gccgggagtt ggagggcttt ccaacccggg ctttggatcc tttcgcccga
     2521 tcgggtgatg atcaggtatt cgacgccggg gacctgccgg gccagttgga tgctttcggc
     2581 ggggctcata acgttaaggg acgtggccag tgcgcccgcg tctgtggcgc ctggggccac
     2641 aacggtggcg ctgaggatct ggtcggccgg ttgtcctgtc cggggatcca cgatatggga
     2701 gtaccagtgg ccgccgatgt tctcaccccg ccggtaattg ccgcttgtcg ctatcgcggc
     2761 gttgccgacc cgcaggcggt cgatggggtt atcattttcc gcgtctgcgg ttggatcgct
     2821 taccaggatg gtctcctttg tatgacccga tacgacgagg tcgccgccga tgttgacgat
     2881 gatggcgtcc gcgccgctgg cggcacgtcc cgcgtctgcc gcgtgttgga tgatatagct
     2941 ttttgcaaag gaattcagca tcaggggtgc attgtcgaga tggatagccg accgggtcgt
     3001 ggcgtcgagg cgccagtggg gctgcctgac cttctcaacg gcggcttcca gttcggtggt
     3061 ggcgggtagc cgttgttctt ttgcggcctg tttccatacc cttgtgatgg tttcggccga
     3121 tgcgtcgagg gcgccgccgg tgcgttggcg ccattggtcg aagagggaga gaacttcgaa
     3181 caactccggg gagatttgca ccggggtccc cgtggttttt tgccagcggc tgaattcgct
     3241 gtttgggtca tagccgctaa ggatcttcga gaggcggtcg atctcggcca ggaccgcgtg
     3301 ttcggcttta tcggaggtct gttgatccgg cacagcaacc tttagctcca tggaagtacc
     3361 caggacattc tcataatgtg agacgaagaa gcgggttttc gccggaggag gggttgccca
     3421 tgcggccggg atcgggagca ggatagcggc cagcgtcggg agcaggataa cggagaggag
     3481 caacgggatg cgtttcatgc aagaaaggtt ttgtttgtgt aaactataaa ataccggtga
     3541 tcgaatttgt taaaagatgt caaatgagca aaaaaaggga cgaattgccg gcatggcaaa
     3601 aaaatttttt gaaaaaagat gccggggtct ttgcaaaact ataaaaatag ttttagattt
     3661 gaactataaa tatagtttaa gtatgaatgc attcaggaaa agaaaggagg aactcgccgg
     3721 agaagggaag acggatggcg agaaaagcgg gaagtctgtc gacggcggcg gggtgaggcc
     3781 gcttaccaag gcggaagagc aggtgatgaa ggtggtctgg caactgggta atggtttgct
     3841 gatggaaatt gtcgagagca tgccgaagcc tgcgccgcat aagaatacgg ttgcgacaat
     3901 attgaagacg cttgtggaca agggttttgt ccgggtggag aatatcggcc gtatccaccg
     3961 gtatcacccg cttgtatcga aggaggctta ttcgcgggac acgctctctt ccgttgcaaa
     4021 ggggtatttc gaggggtcct ttaccaacgt cgtatccttt cttgtcgatg agaagaagct
     4081 gtcggtgaag gacctggaat tactattaca ggaattaaag aaaaacaaga aataatatgg
     4141 aacggctatt cacctttttg tttcagtcgg tgctgatcag cgggatgctg acggcctatt
     4201 attgggttgt attacgcaac cggaagctgc atggctataa ccgatggttc ctgctgggga
     4261 ccgtgggact aagccttttg ttaccgcttg tgcggatcag gtggtcgcct ttcgcctttg
     4321 cgggtgaggc agcgctccca gtggcggcag gccgggcgca ggcggcgggt aatgcagcag
     4381 gtgggagttt tccatggatg gcggttgcgg gttggaccgg catcggggta agcctggtgt
     4441 tgctggggat gctattgctg cgggtagtgc aggtgtaccg gctcaaatcc cggttgtcct
     4501 attcccggat gggtggctat gatctcatcg agacggatga tccgcgggca ccgttctcct
     4561 ttctgaataa tttgttctgg cgtcgcggcg ccgacccgga ggacccggtg aacagaagga
     4621 tactttacca cgagttggcg catatcggcg gcaggcatag ctgggacggt ttgttcgtcc
     4681 agtcggtgtc ctgcgtgttc tggatgaatc cttttttctg gctgatccgc cgggagcttt
     4741 ccgtggtgca tgagtttatc gccgacgcgg caacgggaat ggagggcgac accgaaggtt
     4801 ttgcccggat gcttctgcaa tcgatggatg cagggcgatt cctggaaccc gcacagggct
     4861 tttttcaatc acccatcaaa agaagacttt ttatgatgag caacaatcga cggagccgct
     4921 tttgcctgtt gcgcaaggcg ctcgtattac cggtggtcat ggtcgtgacc gtgtttgttt
     4981 cgtgtacgaa ggagcgggca ccgcttgtgg cggaccgtgt ggatgagatc aaattgaata
     5041 agcaaaagaa ggacgaaaag atgatgaagt ttatgattgt gggcaagggc tcctataacg
     5101 tgaagctatc gaggcctttg gtctttgtaa ggggcagcgg aacggacgaa attaagatca
     5161 agcaggcgac ggagctgatg ctgcagaagt tggaaatgga tgagaaactg atgaagataa
     5221 gattcgatca gaaatgagaa gggagatatt ggcatcgatg tttgtgggtg cggcagtttt
     5281 tgcgaatgcg caaggcattt ataacggcag tgcgacccgg atcaacgacc tggtgcatac
     5341 gaggctggag gtgcggttcg actatgcgca ttcctatgta tacgggaagg tggagctgac
     5401 cttgcggccc cacttctacc ctaccgattc gctgatgctg gatgcgaagc agatgagcat
     5461 ttctgccgtg gcgttggtgg agccggattc ggggcgtggt tcgggtggtt cggtggatgc
     5521 ttttgggcgg gctttgagga agggttcggg gcaggtatcg gcgcggcagt tggggaacgg
     5581 ttcggggcaa gcgttggggc gggtgttggc gcggccgttg gggtttgatt atgacggctg
     5641 gcggttgcgg atccggcttc cgcggcagta ccggcgaacg gagcagtatg ttgtccgggt
     5701 ggtgtatatc gccaaacctg cggaagcgaa
//
LOCUS       DATGFG010001030         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1545008, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001030 DATGFG010000000
VERSION     DATGFG010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545008"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1490)
                     /locus_tag="VKQ52_19590"
     CDS             complement(84..1490)
                     /locus_tag="VKQ52_19590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLZ89471.1"
                     /translation="MFIQQLYTGCLSEAAYYIDSEGEAAIIDPLRDIDAYLRLARERN
                     STIKYIFETHFHADFVSGHLDLAKATGAPIIYGPGTETNFPIHLAKDGEQFRLGNATI
                     EVLHTPGHTVESSCYLVRNADGTPHALFTGDTLFVGDVGRPDLSSGNLGQEELAGMLY
                     DSLRARIMSLPDGVIVYPAHGPGSSCGKNLGPHTHSTLKEEKETNYALRDQTKEEFIK
                     AVTDGLLAPPQYFSINARINKEGYGPLDEVLEAGLQPLDIPSFRKWVREDAAIILDTR
                     RSEVFVAGFVPGSISIGLDGRFAEWAGSLLPFDKPIVLVTSPGEEKESLIRLARVGFD
                     KVKGYLDGGFPAWQEAGEPIDLIIDVEPDELAMDIPFDPHLVVVDVRRETEFADGHVK
                     DAINIPLQDLTDPGNMANLEEDYNLYIHCGGGYRSIIAASLLKRQGIHNLRNVTGGWS
                     RIKDQKGIEVVKEKSVLN"
     gene            complement(1587..1943)
                     /locus_tag="VKQ52_19595"
     CDS             complement(1587..1943)
                     /locus_tag="VKQ52_19595"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ89472.1"
                     /translation="MSLTMNNNSYWMVVNAEGSDGAPVKVDFLNLKKGALILRALNHK
                     LRQQIIRLLDENKKLTVTEIYVHLRLEQSVASQHLAILRRAGIVKTQRDGKFIYYIIN
                     HDRLSEIMKCVDELID"
     gene            complement(2061..2732)
                     /gene="tsaB"
                     /locus_tag="VKQ52_19600"
     CDS             complement(2061..2732)
                     /gene="tsaB"
                     /locus_tag="VKQ52_19600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HLZ89473.1"
                     /translation="MALILNIDTAMAKAGICLAKDGEALAIAENVAQKEHASWLHPAI
                     EQMMLDTGYRLRDLQAVAVTGGPGSYTGLRVGMAAAKGFCFALGIPLIAEDTLRTMAF
                     AAKEQLSDVQLLCPMIDARRMEVFTALYRNDLTPLLPSTAMVVDENSLSGYLEGHRVS
                     FFGDGSDKCKPIILSPHAAFVEINYHAGYLGKLSFLRYLQQEFTGLAYSEPAYTKEFY
                     THTRK"
     gene            complement(2759..>2981)
                     /locus_tag="VKQ52_19605"
     CDS             complement(2759..>2981)
                     /locus_tag="VKQ52_19605"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HLZ89474.1"
                     /translation="NEAYFTWEEEVSTKEEIDTAMRLGTNYPLGPFEWSERIGPGQIA
                     GLLWSLSRTNPAYVPSKALQRAAEALKYD"
BASE COUNT          690 a          822 c          742 g          727 t
ORIGIN      
        1 atgttcgcgg tgaagaatgg gatcgtatct ctcgagtgaa ggagaacctg tcttcccggg
       61 tgagccgggg cctcgatgcc cggctaattc aacacactct tctctttcac cacctcgatc
      121 cctttctggt ccttgatccg gctccagcca ccggtgacat tccggaggtt gtggataccc
      181 tgacgtttga ggagggatgc ggcaataatg ctccggtatc cgccgccaca atggatgtag
      241 agattgtaat cctcttcgag gttggccata ttacccggat cggtcaggtc ctgtaaaggg
      301 atattgatcg catcctttac atgcccgtcc gcgaactcgg tctcgcgccg cacatctacg
      361 acgacgagat gggggtcgaa aggaatatcc atggccagct catcgggttc cacatcgatg
      421 atcagatcga tcggctcccc tgcttcctgc caggcgggaa aacctccgtc gagatacccc
      481 ttcaccttat cgaaaccaac cctcgccagg cggatcaggc tttccttttc ttcgcccggc
      541 gaggtcacca ggacgatggg cttatcaaag ggcaacaggc tgccggccca ttcggcaaac
      601 cggccgtcaa gcccgatgct tatggagccg ggaacaaaac ccgccacaaa aacttccgac
      661 cggcgggtgt ccaggatgat cgcggcgtct tccctgaccc acttccggaa gctgggaatg
      721 tcgagcggtt gtaaccccgc ttccaatacc tcatcgagcg gtccatatcc ctctttattg
      781 atccgcgcat tgatcgaaaa atattgcggc ggcgccaaca ggccgtccgt cacagccttt
      841 atgaactctt ccttcgtctg atcccgcaaa gcataattgg tttccttttc ttccttcagc
      901 gtgctgtgcg tgtgcggacc gagattcttg ccacagctgc tgccggggcc atgcgcggga
      961 taaacgatca ccccgtcagg cagcgacatg atcctggccc ggagggaatc gtacagcata
     1021 cccgccagct cttcctggcc caggttgcca ctactgagat cgggcctccc tacatctccg
     1081 acaaacaacg tgtcgcccgt gaagagggca tgcggagtac catccgcatt acgtacgagg
     1141 taacaggagg actcaaccgt atgcccgggg gtgtgtaaca cctcgatcgt cgcgttccca
     1201 agccggaact gttcgccgtc cttcgccaga tggatcggga aattcgtctc cgtccccggg
     1261 ccatatataa tgggcgcgcc cgtagccttg gccaggtcga ggtggccgct tacaaaatcg
     1321 gcgtgaaaat gggtctcgaa gatatatttg atcgtggaat tccgttcccg ggcaagacgg
     1381 agataggcat cgatatcgcg cagtgggtcg ataatagccg cttctccctc gctatcaatg
     1441 tagtaggccg cttcactaag acagccggta tacaattgct gaatgaacat aaacggaaaa
     1501 tttcggtgaa gttagcaaat ttgccgttcc cgcaatgtcg gccagcttac ggtaaaagag
     1561 ggtgtgccag atcggtcagt cgatggtcag tcgatcaatt catccacaca tttcatgatc
     1621 tcgctcagcc ggtcatgatt gatgatgtag taaataaatt tcccgtccct ttgtgttttt
     1681 acgatacccg cacggcggag aatagcaaga tgctgggatg caacagattg ttcgagacga
     1741 aggtggacat agatctcggt gacggtgagt tttttatttt catccagcag gcggatgatt
     1801 tgttggcgca atttgtgatt caatgctctt aagatcaggg ctcctttctt caggttcaaa
     1861 aaatcgactt ttacaggagc gccatcggag ccctctgcat ttactaccat ccaatagcta
     1921 ttattgttca tcgttaagct catgagcgat taccgtttta aggatttcaa gaagatacgt
     1981 gacaggtaca caggtacggg caaatttatg atcaactctt tgaaaaaaaa tgaccaaatt
     2041 ttgctaatat gataatatag tcattttctt gtatgagtgt agaattcttt ggtataagcg
     2101 ggctcactgt aagccaggcc ggtgaattcc tgttgcaaat atcgtaaaaa agacaatttg
     2161 cccagatacc cggcatggta gttgatctct acgaacgccg catgagggct taaaattata
     2221 ggtttacact tatcgctccc atcaccgaaa aacgacaccc ggtggccctc caggtagccg
     2281 ctgagcgagt tttcgtcgac taccattgcc gtcgacggta ataggggggt cagatcattc
     2341 cggtagaggg cggtgaatac ttccattcgt ctcgcatcga tcatcgggca taaaagttgc
     2401 acatcggaga gttgctcctt tgcggcaaag gccatagttc tcaaagtatc ttccgcgatc
     2461 agcgggatcc cgagggcgaa gcaaaaccct ttggcagcgg ccattccaac acgcaggccc
     2521 gtataagaac cgggcccccc ggtgaccgcc acggcctgaa gatcacgtaa cctataaccg
     2581 gtatccaaca tcatttgttc gattgcagga tgcaaccagg aagcatgttc tttctgtgca
     2641 acattctccg cgattgccag ggcttcccca tccttcgcca ggcagatacc ggcctttgcc
     2701 atcgccgtat caatattcag gatcaacgcc ataagtatcc ctttcgttac ctttcttttt
     2761 aatcatattt taatgcttcc gccgcccgtt gcagcgcctt cgatgggaca taggcagggt
     2821 ttgtccggct caatgaccat aacagaccgg caatctgacc cgggccgatg cgctcactcc
     2881 actcgaaagg acccagcgga taattggtgc caagccgcat cgccgtatcg atctcctcct
     2941 ttgtactcac ctcttcttcc cacgtgaaat acgcttcgtt g
//
LOCUS       DATGFG010001031         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1257585, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001031 DATGFG010000000
VERSION     DATGFG010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257585"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="VKQ52_19610"
     CDS             complement(<1..1320)
                     /locus_tag="VKQ52_19610"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLZ89475.1"
                     /translation="MKKYLLLAALLYFPTLLPFAQTVGYSPSPQNEQAREWFTNAKFG
                     LFIHWGPFSVPGDGEWVMNNRNITVKNYTRLEKFFDPMDFDAHAWVSLVKAAGMKYIT
                     LITRHHDGFSMWDTKYSDFNIMHTPYKKDIVRQMADECHKQGIKLFLYYSLLDWRRDD
                     YSWWTGRTGKGTGRTVHGNWGDYIQFMKNQLTELLTNYGEIGGIWFDGYWDQMAEESK
                     DRKDNDVKVDWHMREIYDLIHRLQPQALIGNNHHMTPLPGEDFQMFEQDVPGENTSGL
                     SFQKISALPLETCATINNSWGFNITDTTYKTPAQIDALLVKSAGNGGNLLLNIGPMPN
                     GEIQPEFIDRLHWMGRWLAVYGESIYQTKGGYLRPQKWGCLTQHGDKLYVHVLQQPNG
                     GVIRLEQFPHGGILKAYRLKDKAAVAVSLKDGVAEIPVSMPTAEEPDP"
     gene            1465..2517
                     /locus_tag="VKQ52_19615"
     CDS             1465..2517
                     /locus_tag="VKQ52_19615"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HLZ89476.1"
                     /translation="MQKHLSLLCLCLLIASLFAKLPAQNIPLKDWTPLSEDNKNTPPI
                     DTVFQGKPCVKLDGHAVAAIWNKKASLKNFRLELDIAGSVMSGVGFHVSDEQNYQFLY
                     FRPGYGGTIEAIQYIPIYNGALSWVFYGAYQASADIHKGQWFHAAIEVRGDNLKVFTN
                     GNTRPDMEIRMLRSEADRGSVLLRTMFGEAYFANLTWRDLPETITAWEISEQLPVPRT
                     YGYEQVKTVKQWRAVNEPGDDYVNLSRYFKTLEGTVIARHIIHADSAGNKLLSFDFTG
                     TLHVFLNGKEIYTYDKYKLDRVEADTYRIRLPLQKGDNELVFITQGDGFIFGKGYNSL
                     GRIQHQNWGFIAAINN"
BASE COUNT          592 a          716 c          633 g          591 t
ORIGIN      
        1 ggggtcgggc tcctccgcgg tcggcatgga tacgggaatt tccgccacgc catcctttag
       61 cgacaccgcc acggcagcct tgtctttcaa ccggtatgcc ttcaggatac ctccatgagg
      121 gaattgctcc aaccgtatga cacccccatt cggctgttgc aggacatgta catacaattt
      181 gtcgccatgc tgggtgagac atccccattt ttgcggccgg agatagccgc cctttgtttg
      241 atagatactc tcgccataaa cagccagcca cctacccatc caatgcaggc gatcgataaa
      301 ttccggctgg atctcgccat tgggcatggg accgatattc aataacagat ttccaccgtt
      361 gccggccgat ttgaccagca gggcgtcgat ctgcgcgggc gtcttataag tggtgtcggt
      421 gatattgaac ccccaggagt tgttgatggt ggcacaggtc tccagcggca gagccgatat
      481 cttctggaag ctgagcccac tcgtgttctc accggggaca tcctgctcga acatctggaa
      541 atcctccccc ggcagcggcg tcatatgatg attgttcccg atcagcgcct gcggctgcaa
      601 ccggtggatc aggtcgtaga tctcccgcat atgccagtcg accttcacgt cgttgtcctt
      661 ccggtccttg ctttcctccg ccatctggtc ccagtagccg tcgaaccaga tgccgccaat
      721 ctccccatag ttcgtcagca actcggtcaa ttggtttttc atgaactgga tatagtcgcc
      781 ccagttgcca tgcaccgtac ggccggtccc cttgcccgtt cgccctgtcc accaggaata
      841 gtcgtcgcgg cgccagtcga gcagggaata ataaaggaac aatttgatgc cctgtttatg
      901 acactcatcg gccatctggc ggacaatgtc ctttttatag ggcgtatgca tgatattgaa
      961 atccgaatat ttcgtatccc acatgctgaa gccgtcatga tggcgggtga tgagggtaat
     1021 gtatttcatc ccggcggcct ttacaagaga tacccaggca tgcgcgtcga aatccatcgg
     1081 gtcgaagaat ttttcgagcc gggtatagtt cttcaccgtg atattgcgat tgttcattac
     1141 ccattccccg tcgccgggaa cgctgaacgg cccccaatga atgaacaacc cgaatttggc
     1201 gttggtaaac cattcccgcg cctgttcatt ttgaggcgaa ggcgaatagc cgaccgtttg
     1261 ggcaaagggc aggagggtgg ggaagtagag caacgcggcg agtaacaggt attttttcat
     1321 aggataaaat taccgaaaac aattctattt tcattgatac gtaatgcctt atcatcttga
     1381 acacacttga cagaacttga acaggctttg aaacacccct ccctgccccc gccgctagct
     1441 tcgtgcgcat caaattggtt gtatatgcaa aaacacctgt cgttgctctg cctttgcctg
     1501 ctgatcgcat ccctgttcgc aaaactgccg gcgcaaaata tcccgctcaa agactggacg
     1561 cccctgtctg aggataacaa aaataccccg cccatcgata ccgttttcca gggtaagccc
     1621 tgcgtaaaac tcgatggcca tgccgtcgcg gccatctgga acaaaaaggc cagtttaaag
     1681 aattttcggc tggaactcga tatcgccggg tccgttatgt cgggtgtcgg tttccacgtc
     1741 agcgatgaac agaactacca gttcctgtat ttccggccgg ggtatggcgg tacgatcgaa
     1801 gccattcaat atatacccat ctacaatggg gcgctcagct gggtcttcta tggcgcctac
     1861 caggcatctg ccgatatcca caaagggcaa tggtttcacg cggccatcga agtgaggggc
     1921 gacaacctga aggtatttac caacggcaac acccgtcccg atatggagat ccggatgctg
     1981 cgcagcgagg cggacagggg ctcggtcctg ttgcggacca tgttcgggga agcctatttc
     2041 gccaacctca cctggcgcga tcttcccgag accatcaccg catgggagat atccgaacaa
     2101 ctgcccgtac ctcgtaccta cggctacgaa caggtaaaaa cagtcaaaca atggagagcg
     2161 gtcaacgaac cgggcgacga ctacgtgaat cttagccgct attttaagac cctcgagggg
     2221 accgttatcg ccaggcatat catccacgcc gacagcgccg gcaacaaact cctgtctttt
     2281 gattttacgg gtaccctgca cgtctttctc aacgggaaag aaatatatac ctatgacaaa
     2341 tacaagttgg accgcgtcga ggccgatacc taccgcatcc gtctacccct gcaaaaagga
     2401 gataatgagc tcgtctttat cacccaggga gacggattta ttttcggcaa agggtataat
     2461 tcactgggcc ggatccagca ccagaactgg ggatttattg ccgcgataaa caactgatat
     2521 atcccgcatc tc
//
LOCUS       DATGFG010001032         4666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_187310, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001032 DATGFG010000000
VERSION     DATGFG010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187310"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VKQ52_19620"
     CDS             complement(<1..1086)
                     /locus_tag="VKQ52_19620"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HLZ89477.1"
                     /translation="MKEQTNIPTGKVERASRFVATGLKVGTNYLKHYTKKLIDPSTSR
                     DELHADNAEDIYDTLSGLKGSALKVAQMLSMDKGMLPKAYTERFAMSQYSAPPLSGPL
                     VVNTFVKTLGKTPSQLYDKFEMQASNAASIGQVHKAVKWGKELAVKIQYPGVANSVKS
                     DLRIVKPFAIRIVGMNEVDMDKYFDEIEGKLLEETDYKLELRRSMEVSAQCAHIPNLV
                     FPVYYPELSSDRIITMDWLQGYHLKEFLQRNPSQEARDAIGQALWDFYQFQVHVLRKV
                     HADPHPGNFLMREDGTVGVFDFGCIKEIPEDFYVNYFLLVDKEVLKDEARRREIYTNL
                     EMIHPSDTEKEITFFSGLFQKMIDLLTL"
     gene            complement(1163..1792)
                     /locus_tag="VKQ52_19625"
     CDS             complement(1163..1792)
                     /locus_tag="VKQ52_19625"
                     /inference="COORDINATES: protein motif:HMM:NF036524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HLZ89478.1"
                     /translation="MDKKIIRDAYIKYWLENGKKPVSVYTFCQQLNTSEPEFYQIYAS
                     FDAVEKDIWRNFFEETQEKLKEDETYRNYSAREKLLSFYFLWVQQLRENRSYILLQKE
                     KYLAPPLHLDKLEAFRAAFYDYIRDLIKEGYQTGEVKERKFVSDQYVHGFWLQALFVL
                     QYWIKDDSDRFEMTDAAIEKAVNLSFQLISSSTLDSILDFGKFVFARKG"
     gene            complement(1962..2873)
                     /locus_tag="VKQ52_19630"
     CDS             complement(1962..2873)
                     /locus_tag="VKQ52_19630"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLZ89479.1"
                     /translation="MRQPLLFLLVITAGLLHGLECGAQTRIIDMHIHSYTEATFQTPA
                     LDFYGNSGSSSAELHRRQTFAEFDRYHIVKAMVCGSPESVDEWVAKDSSHRIIRGITM
                     DSSTDNGMDSAKFEAMIKAGKIQVFGEVGAYYGGTTLNDPVWQPYLRLCQKYDIPVAV
                     HTDGGEPGETYTATPKARLRLGDPFLIEDVLVHYPRLRIYLMHSGEQDYEHVLRLMAH
                     YPQLYSDLGAILWVEPLDQRYATEFLKAAKQEGYLNRVMYGTDQMRWPQAIGKSLRFF
                     GKLPFLTPQDKEDILYNNAARFLRLAP"
     gene            complement(2957..4159)
                     /locus_tag="VKQ52_19635"
     CDS             complement(2957..4159)
                     /locus_tag="VKQ52_19635"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HLZ89480.1"
                     /translation="MRKRSVIICHFIAAIATAALWGCQAPDNSKAGAPGADSLAAVSD
                     STGWALLSFTKVDSVNPVLQPGTGSFIDPIRQRQVAWEEKDVFNPAIVVKDNKVYMLY
                     RAQDKIGKPGGTSRIGLAVSSDGLHFTRAAAPVLYPAEDAEKRYEWEGGCEDPRVVQD
                     GQGVYYMTYTAFDGDKARLMVATSPDLVHWTKRGHVFAKADGGKYVDKWTKSGSIVSR
                     YEADGRVVATKIDGKYWMYWGDQFIWAATSDDLVNWTPVEKRPGEADPIALKGQALAM
                     PDLKIVVPTRSKRFDSDLVESGPPAMLTDKGILLIYNSRNVPAIGDTALPEGTYAASQ
                     VLLDGKDPIKVVHRLDSYFMRPDKPYEITGQVNEVCFLEGLVRFHGQWLLYYGTADSK
                     IAVASRAE"
     gene            complement(4199..>4666)
                     /locus_tag="VKQ52_19640"
     CDS             complement(4199..>4666)
                     /locus_tag="VKQ52_19640"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HLZ89481.1"
                     /translation="LWLRRDGTNSNNYMVSFNRWNGYKFQLQGGNLPFLTVQTSTGYH
                     DVDDGGTSVDGPNVWIHVAVSFTPGTEKFYIQGKLVRTVAVTGTPVPVPSAIDLSIGN
                     ELPKAVYNLTDTNNPNYFWGGDYFMGALDDIRLYNTALTDAQILSIYTDESSL"
BASE COUNT          982 a         1415 c         1164 g         1105 t
ORIGIN      
        1 aagggtcagc agatcgatca tcttctggaa aagacccgaa aagaacgtta tctctttttc
       61 cgtatcgctc ggatggatca tttcgaggtt ggtataaatc tcccgccgtc tggcctcatc
      121 cttcagcact tccttgtcca ccagcaggaa ataattgacg taaaagtcct ccgggatctc
      181 cttgatacag ccaaaatcga acaccccgac ggtgccgtcc tcccgcataa ggaaattccc
      241 gggatgcggg tccgcatgca cctttctcag gacatgcacc tggaactgat agaaatccca
      301 aagcgcctga ccgatggcat cacgggcttc ctgggatggg ttccgttgca ggaattcctt
      361 caggtggtaa ccctgcagcc agtccatcgt gatgatccgg tcgcttgaca gctcgggata
      421 atacaccggg aatacgagat tggggatatg cgcgcactgg gccgagacct ccatgcttcg
      481 ccgcagctcc aatttatagt ccgtctcttc gagcagcttt ccctctatct catcaaaata
      541 cttgtccatg tccacttcgt tcatcccgac gatccggatc gcaaaaggct tcacgatgcg
      601 caggtctgat ttgacgctat tcgccacccc agggtactgg atcttcactg ccagttcctt
      661 gccccatttt acggccttgt gcacctggcc gatggacgcc gcgttcgaag cctgcatctc
      721 gaacttgtca tacaattgcg aaggtgtttt cccgagggtc ttgacaaagg tattcaccac
      781 cagcggccct gagagaggcg gggcgctgta ttgggacatc gcaaaccgtt cggtataggc
      841 cttgggaagc atgcctttat ccatgctaag catctgcgcg accttcagcg cacttccttt
      901 cagaccgctg agcgtatcat agatatcttc cgcattgtcg gcatggagct cgtccctgct
      961 ggtagaggga tcgatgagct ttttcgtata gtgcttgaga taattggtgc ccaccttcaa
     1021 tcccgtggcg acaaaccggc tcgcccgttc aactttcccg gtggggatat ttgtctgttc
     1081 tttcattatc ttgatattac cgctgggtca tccaccggct atcggtgatc cctggcatca
     1141 tctaccggca atcccttcct ccctatcctt tcctggcaaa aacgaatttc ccgaaatcca
     1201 aaatactgtc gagggtactg ctgctgatca gctggaagct gagattcacg gctttttcga
     1261 tggcggcatc cgtcatctcg aagcggtcgc tatcgtcctt gatccagtat tggagcacga
     1321 agagcgcctg tagccagaaa ccgtggacat attgatcgga gacaaatttt cgttccttga
     1381 cctcgccggt ctggtatcct tccttgatca ggtcgcggat ataatcataa aaggcggcac
     1441 ggaacgcctc cagcttgtcc agatgcaggg gcggagccag gtatttctcc ttttgcagca
     1501 ggatatagct ccggttctcg cgtagctgct gtacccaaag gaagtagaag gaaagcagct
     1561 tttcccgggc ggaatagttc cggtatgtct cgtcttcctt taacttttcc tgcgtttctt
     1621 cgaaaaaatt gcgccagatg tctttctcca ccgcatcgaa agaggcataa atttgataaa
     1681 actccggctc ggaggtgttg agttgctggc agaaggtgta taccgaaacc ggctttttac
     1741 cattttccaa ccagtatttg atatacgcgt cgcggatgat ctttttatcc atggttgagg
     1801 atttcgtgtg tcactataac acgcgcccca gccgtcttgt ttgccggcga ttcaaaaatc
     1861 cggcaaaaaa tgcgcgcccg gcccctcagg gcgattggat ccgcgtaccc gggaagataa
     1921 tcccgcaaaa atcatcctat tgcggcaatc gcagaaagcc cctacggcgc caatcgcaga
     1981 aagcgggcgg cattgttata cagaatatct tccttatcct gcggcgtaag aaagggcagt
     2041 ttcccaaaga agcggaggga tttcccgatg gcctgcggcc agcgcatctg gtccgtacca
     2101 tacatcacac ggttcaggta cccttcctgt ttcgccgctt tcaggaattc ggtggcatag
     2161 cgttggtcga ggggctcgac ccataggatc gcgccgaggt cggaatatag ctgtgggtag
     2221 tgggccatca gccgcaggac gtgttcgtag tcctgctcgc cggaatgcat gagatagatg
     2281 cggagccgcg ggtagtggac cagcacatct tctatcagga aggggtctcc cagccgcagc
     2341 cttgccttcg gtgtcgcggt ataggtctcg ccgggctcgc cgccgtcggt gtgtacggcc
     2401 accgggatat catatttctg gcaaagccgc agatagggct gccagacggg gtcgttcaga
     2461 gtcgtgccgc cgtagtaggc gcctacttca ccaaacacct ggatcttccc cgcttttatc
     2521 atggcttcga acttcgcact gtccatccca ttgtccgtcg aagaatccat cgtgatcccc
     2581 cggatgatcc ggtgactgct gtctttggct acccactcat ccacgctctc cggtgaaccg
     2641 caaaccatcg cttttacgat atggtatctg tcgaactcgg cgaaggtctg ccgcctgtgc
     2701 agctccgcgc tggaggaccc gctgttgccg taaaaatcca gcgccggcgt ttggaacgtg
     2761 gcctccgtgt aggaatggat atgcatgtcg atgatgcggg tctgggcgcc gcactcgagg
     2821 ccgtgcagta agcccgccgt gatcaccaac agaaaaagca ggggttgtct catgatggga
     2881 agtctggttc ctgcaattta acccaaattt cgaaggttcc cggccggacc ccgccccccc
     2941 cgctcaatct cccttctcac tctgccctgc tcgccaccgc gatcttcgaa tccgcggtcc
     3001 cataatacag cagccactgc ccatgaaacc tcaccagccc ctccaaaaaa cacacttcgt
     3061 tcacctggcc ggtaatttca tacggcttat cgggccgcat aaaataacta tccagccggt
     3121 gcaccacctt tatcgggtcc ttcccatcca gcaggacctg cgaggcggcg taagttccct
     3181 cgggcaacgc cgtatcgccg atggccggca cattgcggct gttatagatc aggaggatgc
     3241 ccttgtccgt cagcatggcc ggcggccccg attccaccag gtcgctgtca aaccgcttgc
     3301 tgcgcgtggg caccacgatc ttcagatcgg gcatggcaag cgcctggccc ttcagggcga
     3361 taggatccgc ttccccagga cgcttttcca ccggcgtcca gttcaccaga tcgtccgaag
     3421 tagccgccca gataaactga tcaccccaat acatccaata cttcccgtcg atctttgtcg
     3481 ccaccaccct tccgtcagcc tcgtatctcg atacgatgga gcccgacttc gtccacttat
     3541 cgacatattt cccgccatcg gcctttgcaa acacatgccc ccgcttcgtc cagtggacaa
     3601 gatcgggtga ggttgccacc atcagccgcg ccttatcgcc gtcaaatgcc gtatacgtca
     3661 tataatagac accctgcccg tcctgcacga cccgcggatc ctcgcagcct ccctcccatt
     3721 catagcgctt ctccgcatct tccgccggat acaacaccgg cgccgcggct cgggtaaaat
     3781 gcagaccgtc gctgctcacc gccagtccga tgcgcgaggt tcctcccggc tttccgatct
     3841 tatcctgcgc ccggtacaac atatagacct tgttatcctt caccacgatc gccggattaa
     3901 agacatcctt ttcctcccag gccacctgcc gctgccggat ggggtcgata aagctcccgg
     3961 tcccgggctg caacacgggg ttcacgctat ccaccttggt gaacgacagg agcgcccagc
     4021 cggtggagtc actcaccgcc gcaaggctgt ccgcccccgg tgcacctgcc ttcgaattat
     4081 ccggcgcctg acagccccaa agggccgcgg tggcgatggc cgcgatgaaa tgacaaatga
     4141 taactgatct tttcctcatt gacatagtgt tttaaatatt cgcttagcgg ccttcgatct
     4201 acaaactgct ttcgtccgtg taaatagata agatctgtgc gtcggtgagc gccgtattat
     4261 acaatcgaat atcgtccaac gcgcccataa aataatcccc accccaaaaa taattcggat
     4321 tattggtatc cgtcaggtta tagacggcct tcggcaactc gttgccgatg ctgagatcga
     4381 tcgccgaagg caccggcacg ggtgttcccg tcaccgcaac ggtccgcacc agctttccct
     4441 ggatatagaa cttttccgtt ccgggtgtaa agctaacggc cacatgtatc cacacattgg
     4501 gtccgtcgac cgatgtcccg ccgtcatcca catcatgata gcccgtgctc gtctgtaccg
     4561 taaggaaggg caggttgccg ccctgcagct ggaatttata cccgttccac cggttgaacg
     4621 agaccatata attattggag ttggtgccgt cccttctcaa ccaaag
//
LOCUS       DATGFG010001033         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1082900, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001033 DATGFG010000000
VERSION     DATGFG010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082900"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /gene="trpB"
                     /locus_tag="VKQ52_19645"
     CDS             <1..1215
                     /gene="trpB"
                     /locus_tag="VKQ52_19645"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HLZ89482.1"
                     /translation="LFFMIKERISKSPDERGYYGKFGGAFIPEMLYPNVEELRTRYLE
                     IMHEAAFQKEYQGLLRDYVGRPSPLYLAGRLSAKYNAPIYLKREDLNHTGAHKINNTI
                     GQILLAQRLGKRRIIAETGAGQHGVATATVCALKGIECIVYMGEKDIERQAPNVARMK
                     MLGAKVVPATSGSKTLKDATNEAIRDWINNPVDTHYIIGSVVGPHPYPDMVARFQSVI
                     SEEIRVQLKEKTGSELPTHVIACVGGGSNAAGAFYHFLEEGEVELIAVEAAGHGIHSG
                     LSAATTQLGKPGILHGSRSYVMQTEDGQVVEPHSISAGLDYPGIGPLHAYLFDSGRGT
                     FLSATDEEAVAAAFELTRLEGIIPALESAHAVAALDQLELNEKDTVVLCLSGRGDKDM
                     ATYMRAMEGGTI"
     gene            1212..2015
                     /gene="trpA"
                     /locus_tag="VKQ52_19650"
     CDS             1212..2015
                     /gene="trpA"
                     /locus_tag="VKQ52_19650"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HLZ89483.1"
                     /translation="MSRITELFKRKRNRVLNVYCTAGFPRLDSTLEVMKALQENGADL
                     IELGMPYSDPLADGPVIQASSTVALSNGMTLAKLFGQLAEMRESIRVPVLLMGYMNPV
                     LQYGFERFCEAAAGAGVDGLILPDLPMYEFETEYGPVIRRYGLDFVFLVTPETSEARI
                     RQVDALSTGFLYAVSSSSTTGKDKDMGEQEAYFTRLQEMKLRNPVLIGFGIRDRGSFD
                     AACRHANGAIIGTAYIRALEGAAGIGAATKKFLSGILEPTAGVAGEGKK"
BASE COUNT          433 a          444 c          737 g          415 t
ORIGIN      
        1 ctatttttta tgataaagga aagaatcagt aaatcgcccg atgagcgagg gtattatgga
       61 aagttcggcg gggcgttcat cccggagatg ctttacccca atgtggagga actgcgtacg
      121 cgctacctgg agatcatgca tgaggcggcg tttcagaagg aataccaggg tttgttgcgg
      181 gattatgtag gccggccttc gccgttgtac ctggcggggc ggctgagtgc gaagtacaat
      241 gcgccgatct acctcaaacg cgaggacctc aatcataccg gggcgcataa gatcaacaat
      301 acgatcggcc agatcctgct ggcgcagcgg cttggcaagc ggcggatcat cgccgagacg
      361 ggggcgggcc agcatggggt ggctacggct acggtgtgcg ccctgaaggg gatcgaatgt
      421 atcgtgtata tgggagagaa ggatatcgag cgtcaggcgc cgaatgtggc ccggatgaag
      481 atgctggggg cgaaggtggt acccgcgacc agcgggagca agacgctgaa ggatgcgacg
      541 aatgaggcga tccgcgactg gatcaacaac ccggtggata cgcattatat cattggaagc
      601 gtggtggggc cgcatcctta cccggatatg gtggcgcggt tccagagtgt tataagcgag
      661 gagatccggg tgcagctaaa ggaaaagacc gggagtgaat taccgactca tgtaattgcc
      721 tgtgtcggcg gcggcagcaa tgcggcgggc gctttttatc atttcctcga ggaaggggaa
      781 gtggaattga tcgccgttga agcggcgggg catggtatcc atagtgggct gagcgcggcc
      841 acgacgcagt tgggaaaacc cgggatcctg catggaagcc gcagctatgt gatgcagacg
      901 gaagacgggc aggtggtgga accgcatagt atctcggcgg gattggatta ccccggtatc
      961 ggcccgcttc atgcctattt gttcgacagc ggccggggaa cgttcttaag cgccacggat
     1021 gaagaggcgg tggcggcggc cttcgagctg acaaggctgg aagggatcat cccggcgctg
     1081 gagtcggcgc atgcggtggc ggcgcttgat cagctggaat taaatgaaaa ggatacggtt
     1141 gtgctctgtc ttagcgggcg gggcgataaa gacatggcga cgtatatgcg ggccatggaa
     1201 ggaggtacta tatgagcagg ataacggaat tgttcaaacg gaagcgtaac cgggtgttga
     1261 atgtctattg tacggcgggt tttccgcggc tggacagtac gctggaagtg atgaaggccc
     1321 tgcaggagaa tggggccgat ctgatcgagc tggggatgcc ttacagcgat ccgctcgcgg
     1381 acggcccggt gatccaggcc agcagcaccg ttgctttgtc gaatgggatg acccttgcga
     1441 agctgttcgg gcagttggcg gagatgcggg agtcgatccg ggtgccggtg ctgttgatgg
     1501 gatatatgaa ccctgtgttg caatatggat ttgagcggtt ttgcgaagcc gcggcggggg
     1561 ccggggtgga tggactgata ttgccggacc tgccgatgta tgagttcgag acggaatacg
     1621 ggccggtgat ccggcgatac gggctggact ttgtctttct cgtgacgcct gagacatcgg
     1681 aggcgcggat ccggcaggtg gatgcgttga gcacgggttt tttgtatgcg gtgtcctcct
     1741 cttccacgac ggggaaggac aaggatatgg gggagcagga ggcatatttt acacggttgc
     1801 aggagatgaa gctgcgcaac ccggtgctga tcggttttgg tatccgggac agggggagtt
     1861 ttgatgcggc gtgccgtcat gcgaatggag cgatcatcgg gacggcgtat atccgggcgc
     1921 tggagggggc agccggtatc ggggcggcca cgaaaaaatt cttatcgggg attttggagc
     1981 cgacggccgg cgttgccggg gagggtaaga aatgaaactg gtgatcata
//
LOCUS       DATGFG010001034         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1657323, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001034 DATGFG010000000
VERSION     DATGFG010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657323"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1289
                     /locus_tag="VKQ52_19655"
     CDS             102..1289
                     /locus_tag="VKQ52_19655"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ89484.1"
                     /translation="MHSWQLIFYISCGLVFYNYAGYAAVVYLLNKLTGSRQSADEDPD
                     FRPTVAFIVAAFNEQDVIKQKIVNSLEQDYPTDKIEYIFVTDGSSDHTAEIVTQSPGI
                     RLLHRPERGGKSAALNRAVAKATQDIIIASDANTLLNREAVKNIVRHYRSEKTGGVAG
                     EKKVLAAASGAEDVGAGEGLYWKYESALKKLDADFYSVVGAAGELFSLRRELYEPLPD
                     NVILDDFVLSLRVTQKGYRVRYEPDAYAMELPSFSMKDEQKRKIRIAAGGFQAIGMLP
                     HLLAFWKYPRLTFLYVSHRVLRWTLSPLCLMLALVANAFLLPAGPFYQLLFTAQLLFY
                     GVSFIAWRSPAIRDKVKLAKLAYYFTFMNLSVILGFFRFLRGGQSATWEKAKRTLEPI
                     SGD"
     gene            1436..>2549
                     /locus_tag="VKQ52_19660"
     CDS             1436..>2549
                     /locus_tag="VKQ52_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89485.1"
                     /translation="MDLLYYIRVVLKRKWIIIGVAIIAGVVAWYFMRNEPKSYKSVTQ
                     ISTGFTISDEIRLGENFSLYDADVKFNNAIATFTSPTVINLLSYTLILHDLGNPNPFR
                     VPSPEKRKLPLYQAVNQEQARKDFSDKLETITILSSFNPDEKKLLEFLGLYGYDYRTL
                     NKALTITRLQGTDYIQIEFLSENPELSAFVVNTVFQEFLRYYNTTRGHRSQEGIDTLR
                     SLMDKRKQELDAKNALLRGEAPVGVAADSRKLADLTNLERALTDEKARQINLYSNLRK
                     VNQRLANMGVDAQATSNTAVTPPDNTNSEVLMLRKAMNDAYMAYINSGSTDKNLLNRY
                     NQLKTEYQNKIINSNANVYKSPDGTKTDDPTVKKADL"
BASE COUNT          663 a          816 c          554 g          516 t
ORIGIN      
        1 taaaagagat aggcgatttt ttctaaaaat gacacttact tatataattt tatgcgcgta
       61 acgcgttctg acggctcaaa tgtaatacaa aataatagga catgcattcg tggcaattga
      121 ttttctacat ctcctgtgga ctggtcttct acaactacgc aggatatgcg gctgtcgtct
      181 atcttcttaa caaactcacc ggcagcagac agtcagcaga cgaagatccg gacttccgcc
      241 ccaccgtcgc cttcatcgtc gcggccttta acgagcagga tgttatcaaa caaaagatag
      301 tcaactcatt agaacaggac tacccaacgg ataagatcga atatatcttc gtcaccgacg
      361 gctcctccga ccacaccgcg gagatcgtta cccagtcacc cggcatccgc ctcctgcacc
      421 gccccgaaag aggaggtaaa tccgccgcac tcaaccgtgc cgtggcaaaa gcgacacagg
      481 acatcatcat cgccagcgac gccaacaccc tgctcaacag ggaagccgta aaaaatatcg
      541 tccgccacta tcgctcggaa aagaccggcg gcgtagccgg cgaaaaaaag gtcctcgccg
      601 ccgccagcgg cgcggaagac gtaggcgccg gtgaaggcct ctactggaaa tacgagtcgg
      661 ccctcaaaaa gctggacgcg gatttctatt ccgtggtcgg tgccgccggc gaactcttct
      721 ccctgcggcg ggaactctac gaaccactcc cggataacgt gatcctcgac gacttcgtcc
      781 tctccctccg ggttacgcaa aaaggctatc gcgtccgcta cgaacccgac gcctatgcaa
      841 tggaactccc ctccttctcc atgaaggatg aacaaaaaag aaagatccgc atcgccgccg
      901 gcggcttcca ggccatcggc atgctgcccc acctcctcgc cttttggaaa tatccccgcc
      961 tcaccttcct gtacgtctcc caccgcgtcc tccgctggac cctgagcccc ctatgcctga
     1021 tgctcgcgct cgtcgccaac gccttcctcc tgcccgccgg cccgttttac cagctcctgt
     1081 tcaccgcaca gctgcttttt tacggggttt ccttcatcgc ctggcgctcc cccgcgatca
     1141 gggacaaggt caaactggca aaattggcat actattttac gtttatgaac ctgtcggtca
     1201 tcctcggttt cttccgcttc ctccgcggag gacaatccgc cacctgggag aaagccaaac
     1261 gtaccctcga acccatatcc ggggattaaa tagccccccg cacaagggca aaaacttgca
     1321 ccaaaacaaa tcgagtagta tatttacaac tacattcgta cagatatgca gcattttccc
     1381 atccaagttc ctatctggct agcattcaat cacttacaat agcattttaa agttaatgga
     1441 tttactgtat tatatccggg tcgtactcaa gagaaaatgg attatcatcg gggttgccat
     1501 catcgccggc gtggtcgcct ggtacttcat gcgcaacgag ccaaagagct ataaatcggt
     1561 tacccagatc tccaccggct ttaccatctc cgatgagatc aggctcggcg agaacttctc
     1621 cctctatgac gcagacgtca agttcaacaa cgccatcgct accttcacat cgccgaccgt
     1681 tatcaacctt ctttcctata ccctgatcct ccacgatctg gggaatccca accccttccg
     1741 tgtccccagc ccggaaaagc ggaaactacc gctctaccag gcggtcaacc aggagcaggc
     1801 ccgcaaggat ttcagcgata agctcgagac gatcaccatc ctcagttcct ttaacccgga
     1861 cgaaaaaaag ctgctcgaat tcctggggct ctacggctac gactaccgca ccctcaataa
     1921 ggccctgacc atcacacgcc ttcagggcac ggactatatc cagatcgaat tcctgtcgga
     1981 aaaccccgaa ctctccgcct ttgtcgtcaa taccgtcttc caggaattcc tgcgctatta
     2041 caatactacc cgcggtcacc ggtcccagga agggatcgac acccttcgca gcctgatgga
     2101 taaaagaaaa caggaactgg atgccaagaa cgccttgctg agaggggaag ccccggtcgg
     2161 cgtagcggcg gactcccgga aactggccga cctcaccaac ctcgaacgcg ccctcaccga
     2221 cgaaaaagcc cggcagataa acctctattc caacctgcgg aaagtgaatc aacgcctggc
     2281 caatatgggc gtcgacgcac aggcgacatc caacaccgcc gttactcccc cggacaatac
     2341 caacagcgaa gtgctgatgt tgcgcaaggc catgaatgac gcctatatgg cctatatcaa
     2401 cagcggatcc accgacaaga acctgctgaa ccgctataac cagctcaaaa cggaatacca
     2461 gaacaagatc attaattcga atgcaaacgt ctataaatcc cccgacggca caaagaccga
     2521 cgacccaacg gtaaaaaaag ccgacctcc
//
LOCUS       DATGFG010001035         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_743231, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001035 DATGFG010000000
VERSION     DATGFG010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743231"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="VKQ52_19665"
     CDS             <1..1778
                     /locus_tag="VKQ52_19665"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HLZ89486.1"
                     /translation="APTMTSDGMLHYKTADIPMGEFWLRSPTHDKPNDMLDAISGAHI
                     YGKPVIQAEAFTELREAWDESPAMLKTLQDRNYALGINRMVFHVFTHNPWMDRKPGMT
                     LGGVGTFLQRDQTWWPMAKAWIDYTQRCQWLLQQGRPVADIAVFTGEELPRRAVLPER
                     LVSILPGLFGPDRVETETARLSNTGEPTTKFPNGVVHSANMADPTDWLDPLHGYAFDS
                     FNPDALIRLARVHDGRIELPGGGSYALLVLPGATPMTPDPGAMSIAIAKKLSQLVGDG
                     ATILLDPNIHYHDSTVRAIMNRLLKNPRVLKGPYTAHTLTVPPDLIVGVHAPVFQMSP
                     DPTGGNPIGITYTHRTAPGMDIYFVSNQSDYPRSVNLSFRVTGRQPECWDPVTGAIDT
                     TLAWQVADGRTLLHLGMAVHQSLFIVFRHKATAAEPVSRPTETNATTLAGPWTIRFDT
                     AYGGPAQPIRSDTLPDWSRNPDTTIRYYSGSARYTTTFTARRTGTTTLIDLGKLADIA
                     TVAVNGIDCGTVWTNNKLDITKALHPGTNEVRITVANTWANRLTGDQRLPEDRRRTWT
                     STPWRSDGHLLPAGLLGPVTLWQQP"
     gene            complement(1775..>2293)
                     /locus_tag="VKQ52_19670"
     CDS             complement(1775..>2293)
                     /locus_tag="VKQ52_19670"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLZ89487.1"
                     /translation="AVSNLIGLLIQSHDATKGLLSLSVKHLLGREDAAALASRDMHFF
                     LSFVTEVLRYDSPVHNTRRLLTEDVTIGGQVMPKGSTVLVVVAAGNRDPEVFARPEEF
                     DRQRVNNMSMLSFGHGIHGCIAGAYCTELAAETMCYLFHTYKNLRLEESSPEYEPLVN
                     VRMVKSMHLVLS"
BASE COUNT          510 a          857 c          543 g          383 t
ORIGIN      
        1 tggcgcctac catgacaagc gacgggatgc tgcattacaa aacagcggat atccccatgg
       61 gtgagttctg gctgcgcagc cccacccatg acaaaccaaa cgacatgctc gacgccatct
      121 ccggtgcaca tatctatggt aagcccgtca tccaggcgga agcctttacc gaactacgcg
      181 aggcctggga tgaatccccc gcgatgttga aaaccctcca ggatcgtaac tatgcgttgg
      241 gcatcaaccg gatggtcttc catgtcttta cacacaaccc ctggatggac cgcaaacccg
      301 ggatgacgct cggcggggtg gggaccttcc tgcaacgcga ccagacctgg tggcccatgg
      361 cgaaagcctg gatcgactat acccagcgct gccagtggct cctgcaacag ggccggcccg
      421 tggcggacat cgccgtcttc accggcgaag aacttccccg ccgcgccgtg ttacccgaac
      481 gtctcgtctc catcctcccc gggctcttcg gccccgaccg cgtagaaaca gaaaccgcga
      541 ggctgagcaa taccggcgaa ccgaccacaa aatttcccaa cggcgtcgtt cattccgcaa
      601 acatggcgga ccccacggac tggctcgatc cgctccacgg ctatgccttc gattccttta
      661 accccgacgc actgatccgg ctggcccggg tgcacgacgg ccggatcgaa ctgcccggcg
      721 gcggaagcta tgccctcctc gttcttcccg gcgccacccc gatgaccccc gaccccggcg
      781 cgatgagcat cgccattgca aaaaagctgt cacaactcgt cggggacggc gcgaccatcc
      841 tcctcgatcc caacattcac taccacgaca gcacggtccg cgccataatg aaccggctcc
      901 tcaaaaaccc ccgcgtcctc aaaggcccat acaccgccca cacactaacc gtcccaccgg
      961 acctcatcgt cggcgtccat gccccggtct tccagatgag ccctgatcca accggtggca
     1021 accctatcgg catcacctat acgcaccgga ccgcacccgg catggacatc tattttgtct
     1081 ctaatcaatc cgactacccc cgcagcgtaa atctcagctt tcgcgtcacc gggcgtcaac
     1141 ccgaatgctg ggaccccgtc accggcgcca tcgatacaac actcgcctgg caggtcgccg
     1201 atggccgcac cctcctccat cttggcatgg ccgtccatca atcgcttttc atcgtcttcc
     1261 gccacaaagc caccgccgcc gagccggtaa gccgccctac tgaaaccaac gcaaccaccc
     1321 tcgccggtcc ctggaccatc cgcttcgata ccgcctacgg cggccccgcg caacctatcc
     1381 gctccgacac cctgccggac tggagcagga accccgacac taccatccgc tattactcag
     1441 gcagcgcacg ctataccacc accttcaccg cccgccggac aggcacaacc acgctcatcg
     1501 atctcggcaa actggccgat atcgccaccg ttgcggtcaa tggcatcgac tgtggcaccg
     1561 tctggacaaa taacaaactg gatatcacaa aagccttgca ccccggaaca aatgaagtac
     1621 gcatcaccgt cgccaacacc tgggccaacc gcctcaccgg cgaccaacgc ctgcccgaag
     1681 accggcgtcg cacctggaca agcaccccct ggcgttccga cggacacctg ctgcccgccg
     1741 gcctcctcgg gcccgtcacc ctctggcaac agccctagct taaaaccaaa tgcatcgatt
     1801 tcaccatccg cacattgaca agcggctcat actcaggtga cgattcctcc aggcggaggt
     1861 tcttataggt atgaaacaaa taacacatcg tctcggccgc cagctccgta caataagccc
     1921 ccgcaataca gccatggatc ccatgtccaa aggacagcat ggacatattg ttgacacgct
     1981 gccggtcaaa ctcctccggc cgcgcaaata cctccgggtc ccggttcccc gccgctacaa
     2041 cgaccagcac ggtcgacccc ttgggcatca cctgcccgcc gatggtcaca tcctccgtca
     2101 acagccgccg cgtattgtgc accggcgagt cataccgcaa cacctccgta acaaaagaga
     2161 gaaagaaatg catatcccgc gaagcaagcg cggcggcatc ctcccggccg agcaggtgct
     2221 tcaccgaaag gctcagcaaa ccctttgttg catcatggct ctggatcagc agcccgatca
     2281 ggttgctcac ggc
//
LOCUS       DATGFG010001036         6200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_180853, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001036 DATGFG010000000
VERSION     DATGFG010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180853"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VKQ52_19675"
     CDS             <1..367
                     /locus_tag="VKQ52_19675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ89488.1"
                     /translation="YPVFVAEEEGRVVGFSSYGPFRAWPAYKYTIENSVYVSEEQRGK
                     GIGKMLIRPLIESAKAKGYHAIIAGIDSMNDGSIRLHRSFGFVEVAHFKQVGYKFGRW
                     LDLSFMELLLETPEHPVEG"
     gene            complement(401..859)
                     /locus_tag="VKQ52_19680"
     CDS             complement(401..859)
                     /locus_tag="VKQ52_19680"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLZ89489.1"
                     /translation="MFNLRVYGILLGDNKEVLVSDEYIRGNYYTKFPGGGLEFGEGTR
                     DCLKREFKEEMDLEVEVTDHLYTTDFFQMSAFNPEHQIISIYYFVRALEPIKAPLRTK
                     PFDFDERELKIYAERKECETFRFIPRKLFGEESVTLPIDKVVAHLLKTKY"
     gene            complement(861..1799)
                     /locus_tag="VKQ52_19685"
     CDS             complement(861..1799)
                     /locus_tag="VKQ52_19685"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HLZ89490.1"
                     /translation="MIQYFKNVNHQTVEVDHAEDGAWVNILPPLKQQEFTGLSEELDI
                     PLDFLTDSLDIDERARFDDKDNVRLIVIKTPTENNSFNESDAYYITIPICIILTHNHI
                     VTVNSFENGAIKKFLNTFQNRHPDKRNMMVLKVFEKITQTYMEFLKEINQRRNLMEQK
                     LYASNRNEELLELMKIQKSMVYFVTALRSNEMLLLKLARTNFLGLNEEEKEFLDDLAV
                     DMSQALEMANIYTNILSSTLDAFASIINNNMNSVLKRLTSITIILSLPALVTGIYGMN
                     VPIPYADSHYAFYIPILLSVGISIIISWYFMKKKWF"
     gene            complement(1817..2602)
                     /gene="dapF"
                     /locus_tag="VKQ52_19690"
     CDS             complement(1817..2602)
                     /gene="dapF"
                     /locus_tag="VKQ52_19690"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HLZ89491.1"
                     /translation="MNQMHFFKYQGTGNDFIILDNRNWSYTALTTERIRFLCDRRFGI
                     GADGLMLLNPHTGYDFEMKYYNSDGRESSMCGNGGRCLVKFAYDIGIKKTEYRFIAVD
                     GPHLAEIDDDGVVSLKMKDVQDIDDFHGDFILDTGSPHYVKMVTDVMELDVYHKGMDI
                     RYSSDFAKDGINVNFVEQRRPDEIVVRTYERGVEDETLSCGTGVTASALACYHNEAGY
                     NYVKVITKGGKLTVRFDRTAANTYENIWLCGPAVKVFEGSIDN"
     gene            complement(2730..2879)
                     /locus_tag="VKQ52_19695"
     CDS             complement(2730..2879)
                     /locus_tag="VKQ52_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89492.1"
                     /translation="MKKGINAFKKTAKHKRRKAKTQGDVVSHRSKKDLPRSHPYSEAD
                     LSPSR"
     gene            complement(2905..3780)
                     /locus_tag="VKQ52_19700"
     CDS             complement(2905..3780)
                     /locus_tag="VKQ52_19700"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HLZ89493.1"
                     /translation="MQPIPESELFINSRGAVYHLNLRPDELATTVITVGDPDRVTKVS
                     QHFDTIEHRLQHREFVSHTGRIGRKHLTVVSTGIGTDNIDIVLNELDALVNIDLATRT
                     VKKTHTPLTIIRVGTSGSLQRDIPADSFVASTHGLGLDNLLNFYRYDAGEEENQLLHA
                     FATQTQLHHRLAQPYLGSASAALLRHFTTGFHQGITATCPGFYGPQGRVLRLGLAQPD
                     LIDRLTTFSYGPHRITNFEMETAGIYGLGKLLGHSCLSLSAIVANRISKEFSKDGDAT
                     VTRLIRETLRIVEAL"
     gene            3880..3950
                     /locus_tag="VKQ52_19705"
     tRNA            3880..3950
                     /locus_tag="VKQ52_19705"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3912..3914,aa:Cys,seq:gca)
     gene            complement(4446..5195)
                     /locus_tag="VKQ52_19710"
     CDS             complement(4446..5195)
                     /locus_tag="VKQ52_19710"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLZ89494.1"
                     /translation="MKQLANMVAVVTGASKGIGAGIAKGLAVAGAHVVVNYASSKTNA
                     DKVVADIAAAGGKATAVKANVTNEAELTALFEQTEKAFGKVDILVNNAGVWRFEPLER
                     VTAEDFHYMFDTNVLGIFLSCREALKHFNADGGSIINIGSIAGTLAPPGYSVYCAAKG
                     AIDIITKSLSKELAGRNIRVNSINPGMVDTEGNRENGLIGTPIEQHILNSTLLGRIGQ
                     PDDIADVAVFLASEQSRFMTGEILRVTGGMA"
     gene            complement(5223..6032)
                     /locus_tag="VKQ52_19715"
     CDS             complement(5223..6032)
                     /locus_tag="VKQ52_19715"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HLZ89495.1"
                     /translation="MNKIALVTGASSGIGEATGKLLAKNGYKVYGAARRIEKMEDLKS
                     FGVNILPMDVSQDESMVKAVDRIIAAEGTIDVLINNAGFGSFGAIEDVSMKDARYQLE
                     VNVIGLARLCQLVLPYMRKQRSGKIVNITSIGGKMATPLGGWYHASKFAVEGLSDSLR
                     LEVKQFGVDVIIIEPGGVRTEWTGIAVDHAKNTSSKTAYKVLAQKVAQMEAASQNYAE
                     PIEIAEVIRKAIEARNPKARYAKGSSAGVVLSAKRWLSDSRFDKMIMSQYK"
BASE COUNT         1223 a         1411 c         1909 g         1657 t
ORIGIN      
        1 ttatcccgtc tttgttgccg aggaggaggg ccgggtggtc ggatttagtt cctatggtcc
       61 gttccgggcc tggccggcct ataagtatac catcgagaat tccgtctatg tgagcgagga
      121 gcagcggggt aaggggatcg gaaaaatgct tatccggccg ctgatcgagt ccgcgaaagc
      181 gaagggctac catgcgatca tcgcggggat cgattcgatg aatgacggaa gtatccggtt
      241 gcacaggtca tttggattcg tggaggtggc ccattttaag caggtgggat ataagtttgg
      301 ccggtggctt gacctttcgt ttatggagtt gttgctggag acgccggagc atccggtgga
      361 gggttaggga tccgggcggg gccgaagggg ttagggtggg tcagtatttt gttttcagca
      421 gatgtgccac caccttgtcg atgggcaggg tgacgctttc ttcgccgaag agtttccgtg
      481 ggataaagcg gaaggtttcg cattcctttc gttctgcata gatctttagt tcccgttcgt
      541 cgaagtcgaa gggctttgtc cttaacggcg ctttgatggg ttcgagggcg cggacgaaat
      601 aatagatact gatgatctgg tgttcggggt tgaaggcgga catctggaag aagtcggtcg
      661 tgtaaagatg gtcggtgacc tcgacctcga ggtccatctc ttccttaaac tctcttttca
      721 ggcaatctct tgtgccttcg ccgaattcga ggcccccgcc ggggaatttc gtataataat
      781 tcccgcggat gtattcatcg cttacgagga cttccttgtt atcgcccagc aggatgccat
      841 atactctcag gttgaacata tcaaaaccat ttctttttca tgaaatacca gctgatgatg
      901 atggagatac ccaccgacag caggatcggg atatagaatg cgtagtgtga gtcggcatag
      961 ggaatgggta cgttcatgcc gtagatgccg gtgacgaggg cgggcaggga caggatgatc
     1021 gtaatggagg tgagccgctt cagcacgctg ttcatattgt tgttgatgat actggcgaag
     1081 gcgtcgagtg tgctgctgag gatattggta tagatgttcg ccatttccag cgcctgggac
     1141 atgtcgacag cgaggtcatc gaggaattcc ttttcctctt cgttgagtcc gaggaagttg
     1201 gtccgggcca gtttgagaag gagcatctcg ttgctgcgca gggccgtgac gaaatagacc
     1261 atacttttct ggatcttcat cagctccagg agttcttcgt tgcggttgga ggcgtagagt
     1321 ttttgctcca tgaggttgcg gcgctggttg atctccttga gaaactccat gtaggtttgc
     1381 gtgatctttt cgaagacctt cagcaccatc atattccttt tgtcggggtg acgattctgg
     1441 aaggtgttga ggaatttctt tatggcgccg ttctcgaagg agttgacggt gacgatatgg
     1501 ttgtgggtga ggatgatgca gatcgggata gtgatataat aggcatcgct ttcgttaaag
     1561 gagttgtttt cggtgggggt cttgatcacg atcagcctga cgttgtcctt gtcatcgaag
     1621 cgggcccgtt cgtcgatatc gagggagtcg gtgaggaaat cgagggggat gtccagttcc
     1681 tccgagaggc cggtgaactc ctgctgtttg aggggcggga ggatattgac ccaggcgccg
     1741 tcctcggcgt ggtcgacctc tacggtttga tggttgacat tcttgaagta ctggatcatg
     1801 ggttgttggg gtggggctag ttgtcgatac tgccttcgaa gacttttacc gcgggtccgc
     1861 agagccagat attctcatag gtgttggcgg cggtgcgatc gaagcgaacg gtgagtttgc
     1921 cgccttttgt gatgaccttt acgtagttgt agccggcctc gttgtgatag caggcgaggg
     1981 cgctggcggt gacgccggtg ccgcaggaca gggtctcgtc ttcgacgcct ctttcgtagg
     2041 tgcggacgac gatctcgtcg gggcggcgtt gttctacgaa gttgacattg atgccatcct
     2101 ttgcgaagtc gctgctatag cggatgtcca tacctttatg gtagacgtcg agttccatga
     2161 cgtcggtgac catttttaca tagtgggggg agccggtatc gaggataaag tcgccgtgga
     2221 agtcgtcgat atcctggacg tctttcattt tcaggctaac cacgccgtcg tcgtcgatct
     2281 cggcgaggtg gggaccgtcg acggcgatga aacggtattc ggttttttta atccctatgt
     2341 cgtaggcaaa tttgaccagg cagcggcctc cgttgccgca catgctgctc tcgcggccgt
     2401 cggagttata gtatttcatt tcgaagtcgt agccggtatg ggggttgagg agcatgaggc
     2461 cgtcggcgcc gatgccaaag cgtcgatcgc agaggaatcg gatgcgttcg gtggtcaggg
     2521 cggtgtagct ccagttacgg ttgtccagga tgatgaagtc gttgccggtg ccctggtatt
     2581 tgaaaaagtg catttggttc atagcagcgg cgaagatagc tattccgggg gagtttgcct
     2641 agcgggccgg cgcacggcgc cggcgtccga aggaccgaca ttggccctgc cagattgcga
     2701 agggcatagg aaaaggttgc gactcgggct tagcggctgg gggagaggtc ggcctcggag
     2761 taggggtggc tgcggggtag atcctttttc gatcggtggg agacgacgtc gccctgggtt
     2821 tttgcctttc tgcgtttgtg tttggcggtt tttttgaatg cgttgatccc ctttttcata
     2881 ggatgaatgt acgaaaaaag ggggttacag ggcttcgacg atgcgtaggg tttcccggat
     2941 gaggcgggtg acggtggcgt cgccgtcctt tgagaattcc tttgagatgc ggttggctac
     3001 gatggcgctg aggctcaggc aggaatggcc gagcagtttg ccgagaccgt agatgccggc
     3061 tgtctccatt tcgaagttgg tgatgcggtg ggggccgtag gagaaggtcg tgaggcgatc
     3121 gatgagatct ggctgggcga ggcccaggcg gaggacgcgg ccctgggggc cgtagaagcc
     3181 cgggcaggtg gcggtgatgc cctggtggaa gccggtggtg aagtggcgga ggagggcggc
     3241 gctggcgctg ccgagatagg gttgggcgag gcggtggtgc agttgggtct gggtagcgaa
     3301 ggcatggagg agctggttct cttcttcgcc ggcgtcgtac cggtagaaat tgaggaggtt
     3361 atcgaggccg agtccgtggg tggaggcgac gaagctgtct gcgggtatat cccgctgtaa
     3421 ggagccgctg gtgccaacgc ggatgatggt gaggggggta tgggtttttt tcaccgtccg
     3481 ggtggcgagg tcgatgttga ccagggcgtc gagttcgttg aggacgatat cgatattgtc
     3541 ggtgccgatg ccggtggaga cgacggtgag gtgtttacgg cctatccggc cggtatggga
     3601 gacgaattcg cggtgttgga ggcggtgttc gatggtatcg aaatgttggc ttaccttcgt
     3661 tacgcggtcg gggtcgccta cggttataac ggtcgtggcg agttcatcgg ggcggaggtt
     3721 gaggtggtag acggcgccgc ggctgttgat aaagagttcg ctttcaggga ttggctgcat
     3781 atataatggg agatattttt gagtttgttg cgaaattacc ttaattttgc agccactcaa
     3841 aaaattcgct ttttgcgatg attttttggg ttggattttg gtctcgtggc cgagtggcta
     3901 ggcagagctc tgcaaaagct tctacagcag ttcgaatctg ctcgagacct cgcgaaaacg
     3961 cgaacagcgc aaaaaaccgc cgataggcgg ttttttcgtt tgggccgaag tcggcgcgag
     4021 cttcgctcgt ggccgataag gcacaaatga aaccggccgg acgaaggccg gccaggtttg
     4081 cgtgatcgag gctttcaagc ccccccaaga cggcagatca ccggcggcgc ctgagcgtta
     4141 gcgaaggggc cgcgggagga gggccgctgt taagcggatc cgacgaaacg cgggcgcgac
     4201 gaatgtcggc gccgccggta atctgctcga gacctcgaaa tgtggcacat tgccaaaaag
     4261 accgccggaa ggcggtcttt ttatttgccg aaatttgggt tcatcctggg ttttaatgaa
     4321 tagaggtctt caagttgggc agttcgaata tgttagggaa gtttttcgcc attgagggta
     4381 agaggatgtt ttttaggcgt ttgcggcagt tcgatgtctg ctatgggtat tttccatctt
     4441 cctgtttacg ccataccacc cgtgacacgt aatatttcac cggtcatgaa ccgggactgt
     4501 tcagaagcaa ggaatactgc tacgtcggca atatcatcgg gctgaccgat gcgtcccagt
     4561 aacgtcgagt tcagaatatg ttgttctatt ggagtgccta tcagaccatt ttcccgattt
     4621 ccttcagtgt ccaccatacc gggattgatg gagttgacgc gtatgtttct gccggccagt
     4681 tctttggaca atgatttcgt gatgatatcg attgcgcctt tggctgcgca ataaacggag
     4741 tatccgggag gcgccagcgt gcctgctatc gaaccgatgt taataatact accgccatcg
     4801 gcgttaaaat gctttagtgc ttctctacag gacaggaata tgcccaacac gttggtgtca
     4861 aacatgtagt gaaaatcttc ggcggtgacc cgctcgaggg gttcaaatct ccaaaccccc
     4921 gcgttattaa ccaggatgtc gaccttgccg aatgcttttt ctgtttgttc gaataaagct
     4981 gtgagctctg cttcgtttgt tacgttggcc tttaccgctg tggccttacc tccagcggcg
     5041 gcgatgtcgg ctactacttt atcggcattt gtcttcgacg acgcgtagtt tacgactacg
     5101 tgtgcgcccg cgacggcaag gcccttggcg attcctgcgc cgataccttt tgaggcgccg
     5161 gtgacaaccg caaccatatt tgctaattgt ttcatttatg tggcaattta tttttaaggc
     5221 aactatttat actggctcat gatcatttta tcgaagcggc tgtcgctgag ccacctcttt
     5281 gcagacagga ccacgccggc agagcttcct ttagcgtaac gggctttggg attccttgcc
     5341 tcgatagcct tcctgatcac ttctgcaatt tctattggct cagcgtaatt ttgcgatgca
     5401 gcctccattt gggcaacttt ttgcgccaac actttataag ctgttttcga ggaggtgttc
     5461 ttcgcgtgat cgactgcgat acctgtccat tcggttctaa ccccgcccgg ctcgataata
     5521 atgacgtcta ctccgaactg tttaacttcc agccgaaggc tgtcgctcag gccctccacg
     5581 gcaaacttgc tggcgtgata ccagcctccc agcggggtgg ccatttttcc gccgatggag
     5641 gtgatgttta caattttgcc cgaacgctgt tttcgcatgt atggcaacac cagctgacag
     5701 aggcgagcca gacctatcac atttacttcc agctgatatc gggcatcttt catagatacg
     5761 tcctctattg ctccaaaaga gccgaacccg gcattgttga tcaatacgtc gatggtgcct
     5821 tcggcagcaa taatccgatc gactgctttt accatcgatt catcctgcga aacgtccatg
     5881 ggaaggatat tgacgccaaa acttttcagg tcttccattt tctcaattct tcgggccgct
     5941 ccataaactt tatatccatt ttttgccaga agcttgccgg tcgcttctcc aatgcctgag
     6001 gatgcaccgg ttaccagagc tattttattc atatcattta tttatcagat aattctcgcc
     6061 agcgacccaa tcttcactca aatgtcccat ccattggtgg tacatttcat caccgctgtg
     6121 ctgtgtgaat tcaatgttca gctgatttac ttccaagcct agaatgtcgt tgcaaagttc
     6181 tatgagcgcc ttggacaaaa
//
LOCUS       DATGFG010001037         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_136836, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001037 DATGFG010000000
VERSION     DATGFG010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136836"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..677)
                     /locus_tag="VKQ52_19720"
     CDS             complement(171..677)
                     /locus_tag="VKQ52_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89496.1"
                     /translation="MRKYWIESLPGMPPLPPCPKGYSRPDRVTASFIEEGYLVEYFEF
                     PFDRDRTVALFFGPGEFLIRSHPVFSTVQFLDRGCLADFSYGDVLRTLRNFPESKLHY
                     LEMKRRYEQKVADRLTMSALGGDLERLQLLQQTQPWVLQKAPDSLVAGYLGVTPARLQ
                     EMKQICKP"
     gene            complement(704..1528)
                     /locus_tag="VKQ52_19725"
     CDS             complement(704..1528)
                     /locus_tag="VKQ52_19725"
                     /inference="COORDINATES: protein motif:HMM:NF020914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RteC domain-containing protein"
                     /protein_id="HLZ89497.1"
                     /translation="MNRNFGRLQEETGEALQEALKETHPISRYRGCIRILESAGHQLK
                     EMVVTNPFATREDEIHHFKHEAPEIYSQLFYYLELNRLETDRDYCDPKAFLAVLRRKK
                     IAIDEYFQEHDAICRYYSQGSAFQDDYLFIRRPTGQFLGDEIGTFIPPDFTIGTHWFS
                     FIKANMRLRDWIREELAKGEAVWEGESAKRKPKLRWTGTRADLILLLCALSLTGCFNN
                     GELTLKQIMEWGEEEFAFKLGQYHVALGEMSIRKEPVKFLHRLIDLVLRKFDSMTA"
     gene            complement(1596..>2101)
                     /locus_tag="VKQ52_19730"
     CDS             complement(1596..>2101)
                     /locus_tag="VKQ52_19730"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLZ89498.1"
                     /translation="QIIFKEGVIASRILYLEEGLIRSFSIVNGKKASNYFMRQGDIII
                     SVESFFQQIPAYDTIVALEDCVCWGITYDELEETYQKFTKFNTNGRRIVQVYYCKSEA
                     RHRTRQLKKPELMYKEIMEADPDLLSRVRDNYVASYLNVSKATYSGIKRRYVEMLRIS
                     RSKNVQF"
BASE COUNT          496 a          548 c          511 g          546 t
ORIGIN      
        1 tccaggaata ctgggtgaac atgacaggaa tttacaccaa ctgcgtaaat tgggttggcc
       61 atcctccatg aggggcagca tgcggtcaag aatggcttcg gggtcaatct tatgcctcag
      121 taaatcagcg tctactacta aatgtgcgat ctttgacata gttacaggtt ttatggtttg
      181 caaatttgtt tcatctcctg cagtctcgcc ggggtgacac ccaggtatcc tgccaccagg
      241 ctgtcaggcg ccttctgcaa cacccagggc tgtgtctgtt gaagcaattg aaggcgctcc
      301 aggtcaccgc caagggccga catcgtcaac cggtccgcta ccttctgttc ataccgtctc
      361 ttcatttcga gataatgcag ctttgattcg ggaaaattgc gcaatgtccg gagtacatcg
      421 ccgtatgaga aatccgcgag acatccccgg tccaggaatt gaaccgtcga aaagaccggg
      481 tgactcctga tcaggaactc cccgggtcca aagaacaagg ccacagtgcg atccctgtcg
      541 aagggaaact cgaaatactc taccagatat ccttcctcta taaaagaggc ggtcacccgg
      601 tcgggccggg aatacccttt gggacagggc ggcagggggg gcatccccgg caggctttcg
      661 atccaatact tgcgcatatg gcgggtttta atgatttgcg atatcaagcg gtcatcgagt
      721 cgaatttgcg caggacgagg tcaatgagcc ggtgcagaaa ttttaccggc tctttcctga
      781 tcgacatctc acccagcgct acgtgatatt gacccagttt aaaggcaaac tcctcttcgc
      841 cccattccat tatctgtttt agggtcaact ctccgttgtt gaagcagcct gtcagcgaaa
      901 gggcgcataa aagaagaatg agatcggccc gggtgcccgt ccagcgtagt tttggcttcc
      961 ttttcgccga ctcgccttcc catacagcct cgcccttggc caattcctcc ctgatccaat
     1021 cccgcaagcg catattggcc tttataaagg aaaaccaatg tgttccgatg gtgaaatccg
     1081 gcggaataaa ggtcccgatc tcatctccca gaaactgacc ggtgggcctc ctgataaaaa
     1141 gatagtcgtc ctggaacgcc gagccctggc tgtagtaacg gcatatcgcg tcatgctcct
     1201 gaaaatattc gtcgatggca attttctttc gccgcaacac tgccaaaaat gccttcggat
     1261 cgcaatagtc ccggtcggtc tcgagccggt tcagttccag gtaatagaaa agctgactat
     1321 agatctccgg agcctcatgc ttgaaatgat ggatctcatc ctccctggtg gcgaacgggt
     1381 tggtgacgac catctctttc aactgatggc cggcggactc cagtatcctg atacacccgc
     1441 gatagcggct gataggatgg gtttctttta gtgcctcctg cagtgcttca cccgtttctt
     1501 cctgaaggcg gccgaaattc cgattcatgt ggtttcatta atgaatcgaa attagggtaa
     1561 tgataaacga taatcaatac cctaaggcag aaggtctaaa attgaacatt tttactgcgc
     1621 gatatccgca gcatttcaac gtagcggcgt ttgatgccgg agtaggtggc cttgcttaca
     1681 ttgaggtaac tggctacata attatctctt acccggctga gcagatcagg gtccgcctcc
     1741 attatttctt tatacatcag ttccggcttt tttagttgcc tggtacggtg cctggcctcc
     1801 gacttgcaat agtatacctg tactattctc ctgccattag tgttaaattt tgtgaacttc
     1861 tggtaggtct cttccagctc gtcataagtg attccccaac agacacagtc ctccagcgcc
     1921 acaatggtat cgtaggccgg gatttgttgg aagaaactct cgacggaaat gatgatatcc
     1981 ccctgccgca taaaatagtt ggaggctttt ttgccattta ctatcgaaaa gctgcggatc
     2041 aggccctctt ccaggtatag tatgcgggag gctataaccc cttctttgaa aatgatctgg
     2101 c
//
LOCUS       DATGFG010001038         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1199376, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001038 DATGFG010000000
VERSION     DATGFG010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199376"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VKQ52_19735"
     CDS             <1..442
                     /locus_tag="VKQ52_19735"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HLZ89499.1"
                     /translation="AREGIPALLIEKEPFFRGDAYVKTLREKQIDLVVLAGFLWKIPA
                     ALIHAYPGKIVNIHPALLPKFGGKGMYGRFVHEAVIAASEKETGITIHYVDEHYDHGQ
                     PIFQARVLVEKDDTPETIAQKVHWLEYEHFPRVIEEVLNVQNHR"
     gene            509..1252
                     /locus_tag="VKQ52_19740"
     CDS             509..1252
                     /locus_tag="VKQ52_19740"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ89500.1"
                     /translation="MAFSTHKFLLLPLSFFITFLFCGKVRGQEVTIHGTVYNMYRTKP
                     LDGVSVISVLGRGTATDSNGNYAITVSIKDSLYFSYLGRATAKFPVMNINSLNGFDIA
                     LHVDPVELKEVRVMPRNYHYDSLQNRQDYARIFNYRKPGFHPDITGMGVGLDLNQLIE
                     MFNRAKTRRTLAFQRRLVDEEKDGYVDFRFNRSIVLKVTHLEGDELDSFMMRYRPSYD
                     FCRRATDYDLLDYIKLAFHEYQKDRKDRP"
     gene            1272..1826
                     /locus_tag="VKQ52_19745"
     CDS             1272..1826
                     /locus_tag="VKQ52_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89501.1"
                     /translation="MDQLTLGIGTRLQHTQYGPGVIVGVKYAVYLITFIHHGLKEIEK
                     NDPNLDEIIPANVTAEVETQSETERSLLGILRLWGGISEVVPLGDKWKGGQLILQAGD
                     RSIKSKEIPIDIFFHKIVMARDRLRVLEQQINGHKGLSDEEKVNLQQYITRIYGSFTT
                     FNVLFKNKEQWFIGESASQGSQEK"
BASE COUNT          587 a          586 c          490 g          474 t
ORIGIN      
        1 cgcaagggaa ggcatccccg ccttactgat agaaaaagaa cccttcttcc ggggtgacgc
       61 ctatgtaaag acgctccgcg aaaaacagat cgatctcgtc gtgctggcag ggtttttatg
      121 gaagatcccg gcggccctca tccacgccta ccctgggaag atcgtcaaca tccacccggc
      181 gctgctacca aaattcggcg gcaaagggat gtacggccgt tttgtacacg aggccgtgat
      241 cgccgcatcg gaaaaagaga cgggcatcac tattcattat gtggatgaac actatgacca
      301 cggacagccg atcttccagg ccagggtcct cgtcgaaaaa gatgacaccc ccgagaccat
      361 cgcacaaaag gtgcattggc tggaatatga gcacttcccg cgggtcatcg aagaggtgct
      421 aaatgtgcaa aatcatcgtt aaaaatacag ccccgtccgc tggtatatcc ggttttggta
      481 gtaatatcac ctttaaatac tacagtcctt ggcattttct acccataaat tcttactgct
      541 gcccttatcc ttttttatta cattcctttt ttgcggcaaa gtaagggggc aggaagtaac
      601 tatccatgga acggtatata atatgtaccg cacaaaaccc ctggatgggg tcagcgtcat
      661 cagcgtcctg ggaaggggaa ccgccaccga ctccaacggc aactacgcca tcacggtaag
      721 tataaaagac tccctctact tctcctatct cggccgggcc accgccaaat tccccgtgat
      781 gaatatcaac tcccttaacg gcttcgatat cgccctccat gtcgaccccg tcgaattaaa
      841 agaagtgagg gtcatgcccc gaaactacca ctacgactcc ctgcagaacc ggcaggacta
      901 tgccaggatc tttaattacc gtaaaccggg cttccatccc gatataaccg gtatgggcgt
      961 gggcctcgat ctcaaccagc tgatcgaaat gttcaaccgc gccaagacaa gacggaccct
     1021 cgcctttcag cgaagactgg tggatgaaga gaaagacggg tatgtcgact ttcgtttcaa
     1081 ccgcagcatc gtcctgaaag tgacccatct cgagggtgac gaactcgaca gcttcatgat
     1141 gcgctatcgt ccctcctacg acttctgcag acgggccacc gattacgatc tgctggatta
     1201 tatcaaactg gccttccacg aatatcagaa agaccggaag gaccggcctt agtaaaacca
     1261 aaactctcaa tatggaccaa ctgacgctgg gcatcgggac ccggttgcaa cacacccaat
     1321 acggacccgg ggtgatcgta ggcgtgaaat atgccgtcta tctcatcacc tttattcacc
     1381 atggcctgaa agagatcgaa aagaacgatc ccaacctcga cgagatcatc cccgcgaacg
     1441 tcaccgcaga agtagagacc caatcggaaa cagagcgatc cctgctgggt atcctccgcc
     1501 tctggggagg gatctcggaa gtggttccgc ttggcgacaa atggaaggga ggacaactca
     1561 tcctccaggc cggcgacaga tccatcaaat caaaggagat ccccatcgat atcttcttcc
     1621 acaaaattgt gatggcccgc gaccgcctgc gcgtactgga acaacagatc aatggacata
     1681 agggactaag cgatgaagag aaggtcaatc tgcaacagta tataacccgc atctatggtt
     1741 ccttcaccac cttcaatgta ttattcaaaa acaaggagca gtggtttatc ggcgagtctg
     1801 cttcgcaagg ttcacaagaa aaataacctt gcttgcagac tatctaatga ttgaccttcg
     1861 gtcggcagac ctccggtctg ccctcggaag cggggagtag tgccgaacga taaggctgag
     1921 taaagaaaaa cctccctaat ggggaggtcg ccctaatcag ttccataacc attaaacctt
     1981 atcctatttg ttataaaggt aagaattctt ctttttattt tccaagcttt gtgcatgaat
     2041 agtgcaaaac ttgcccgaac tagtattcca acaacttttg tacatattca tccagccttg
     2101 cactggcaag gaaattgccc tccagcatcc cgttaaa
//
LOCUS       DATGFG010001039         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_172654, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001039 DATGFG010000000
VERSION     DATGFG010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172654"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VKQ52_19750"
     CDS             <1..216
                     /locus_tag="VKQ52_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89502.1"
                     /translation="ALSVIVVVMMRIYSSNFEFGKLGFMRTAARAITYTYWYPIPKEA
                     RQIAGSACESDIYCLQTEKTRLLSSTE"
     gene            complement(202..2217)
                     /gene="mutL"
                     /locus_tag="VKQ52_19755"
     CDS             complement(202..2217)
                     /gene="mutL"
                     /locus_tag="VKQ52_19755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HLZ89503.1"
                     /translation="MPDIIQLLPDNIANQIAAGEVIQRPASAVKELMENAVDAGATDI
                     RLFVQEAGKALIQVVDNGQGMSETDARMAFERHATSKIRKIDDLFHIRTMGFRGEALA
                     SIAAVAQVELKTRRPEDEAGTMIEIENSRVVRQEPTALPAGTSIAMKNLFFNVPARRN
                     FLKSNSVEMKHILDEFIRVAISFPSIFFSLTGNGQELMHLEKGSLKQRVVQVLGNSYA
                     SKLVSVQENTDYLNIYGFVGKPDTAKKTRGDQYFFVNNRYIRSAYLHHAVNGAFAEMI
                     PRESFPLYVLFIDLDPAQLDINVHPTKQEIKFEDEKIVYAFVQAAVKHALAQFSITPT
                     LDFDLDPDIQRLDAVSKPVSEEKKASVASSSLYQTFTQKNQAHFIEPTNKSELRHWKD
                     FYEAPGNRQQPAETGSPSPETGPHPTTESGPHSPAESQKQAAGQAAPTAAAPGTTATS
                     GGATGTPAPGNPTATAATPGDWAPTAPTRITPLENAPLQQLLNTYIVTPTNRSFILVH
                     QQAAHERILYERYKTAAAGQQIPSQKSLFPATLTLSAPDAVLLTELLPDLQILGYTIE
                     PFGKDSFIIQGTPADTGQGNEKGALEQLLEQYKNFVSEIRFSKREKLIRTLARQNAVR
                     PGTSLTEKEMRTLVDDLFACAQHNVTPGGDPTYIEFKKDYVERLFGR"
     gene            2288..>3171
                     /locus_tag="VKQ52_19760"
     CDS             2288..>3171
                     /locus_tag="VKQ52_19760"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HLZ89504.1"
                     /translation="MRKFLLVLTLLAGMIRGLGQSSTTLSADAWVDSVFRTLTPDEKI
                     TQLMVIRLSAIDPATHRAIFYDKQVEDAVRRYNVGGICLFQGDPLSQADHINFYQGIT
                     RTPILFCIDAENGLGMRMDSVEGLPRQMMLGATGNPSLIYRYGRLVGGQCRRIGIQVN
                     YAPVVDINNNPDNPVINDRSFGEDKWKVADYGVQYMRGLQDEGVMACAKHFPGHGDVS
                     VDSHFDLPVIGKDRRQLDSLELFPFRALFKAGVGSVMIAHLYIPAIDNEPNRATSLSY
                     NNVTKLLRKQLHYQGISFT"
BASE COUNT          560 a          823 c         1013 g          775 t
ORIGIN      
        1 gcgctgtccg ttatcgtggt tgtcatgatg cgcatctatt ccagcaattt cgagtttggg
       61 aagctggggt tcatgcggac ggcggcgagg gcgatcacct atacctactg gtatcccatc
      121 ccgaaggagg ccaggcagat agccggcagc gcctgcgagt ccgatattta ttgtctgcaa
      181 acggagaaga caaggctcct ttcatctacc gaatagccgt tcgacataat cctttttgaa
      241 ttcgatgtag gtggggtcgc cgcccggcgt gacattatgc tgggcgcagg cgaagagatc
      301 gtcgacaagg gtgcgcattt ccttttcggt gaggcttgtt cccggcctga cggcgttctg
      361 ccgggccagg gtgcggatca gtttttcgcg tttggagaaa cggatctcgc tgacgaaatt
      421 cttgtattgc tccagcagtt gttccagcgc gcctttctcg tttccctggc cggtgtcggc
      481 cggtgtcccc tggatgataa agctgtcttt tccaaaaggc tcgatggtat agcccaggat
      541 ctggaggtcg ggtaagagct ccgtcagcag gacggcgtcc ggtgcggaga gcgtcagggt
      601 tgcggggaac aggcttttct ggctggggat ctgctgaccg gcggcggccg tcttgtagcg
      661 ttcgtacagg atcctttcgt gggcggcctg ctgatggacg aggatgaagc tgcggttggt
      721 gggggtgacg atataggtgt tgagcagctg ttgcagcggg gcgttctcga ggggggtgat
      781 gcgtgtcggg gcggtggggg cccagtcgcc gggagtggcg gcggtggcgg tggggttgcc
      841 cggggccggg gttcctgttg ccccgccgga ggttgcggtt gtccctggag cggcggcggt
      901 gggggcggct tggcctgcgg cctgtttttg gctttccgcg ggggagtgtg ggccggattc
      961 cgtggtgggg tgtggaccgg tttcggggga cggggaaccg gtttcggcgg gttgctggcg
     1021 gttgccgggc gcctcataga aatccttcca gtgacggagc tcgcttttat tcgtgggctc
     1081 gatgaaatgg gcctggttct tttgggtaaa ggtctggtaa agggaggaag aggctacgga
     1141 ggcctttttt tcttccgata ccggcttgct cacagcatcc agccgctgga tatccgggtc
     1201 gagatcgaag tccagggtag gcgtgatgct gaactgcgcc agggcatgtt tcacggcagc
     1261 ctgaacaaag gcatagacga tcttttcatc ttcgaatttg atctcctgtt ttgtcggatg
     1321 cacatttata tccagctgtg cgggatcgag atcgatgaac agcacataga gcgggaagct
     1381 ctccctgggg atcatctccg cgaaggcgcc gttcacggca tgatgcaggt aggcgctgcg
     1441 gatgtaccgg ttgttgacaa agaaatactg atcgcctctt gttttttttg ccgtatccgg
     1501 cttgcctacg aacccataga tattgaggta gtcggtgttc tcctgcaccg acaccagttt
     1561 ggaggcatag gaattcccga gcacctgcac gacgcgttgc ttgaggcttc ctttttcgag
     1621 gtgcatcagc tcctggccat tgccggtgag ggaaaagaag atcgagggga aggagatggc
     1681 gacgcggatg aattcgtcga ggatatgttt catctcgacg gaattgcttt ttagaaaatt
     1741 gcgccgggcg ggcacattga agaaaaggtt cttcatggcg atgctggtgc cggcggggag
     1801 ggccgtgggt tcctggcgga cgacacggct gttctcgatc tcgatcatcg ttcccgcctc
     1861 gtcttccggc cttctcgtct tcagctccac ctgtgcgacg gcggcgatgg aggccagggc
     1921 ctctccgcgg aagcccatcg tccggatatg aaaaagatcg tctatcttcc tgatcttcga
     1981 tgtagcgtgt ctttcaaacg ccatgcgggc gtccgtctca ctcataccct gcccgttgtc
     2041 gaccacctgg atcaacgctt tccctgcctc ctgtacaaag agccggatat ccgttgctcc
     2101 ggcgtccacg gcattctcca tcaactcctt cacggcgctg gccggtcgct ggatgacttc
     2161 tccggcggcg atctggttgg cgatgttgtc gggcaataat tggatgatgt ctggcaattc
     2221 tatcttattt tcgttgaggt tagttccttt attacctatg caatttacgt cgctttacgt
     2281 ctccgatatg agaaaattcc tcctcgtcct gaccctgctg gccggcatga tccgcggcct
     2341 tggccaatct tccactacgc tttccgccga tgcctgggta gacagtgtct tccggacgct
     2401 cactcccgat gaaaagatca cccagctgat ggttatccgg ctttcggcga ttgacccggc
     2461 cacgcatcgt gcgatctttt atgacaagca ggtcgaggat gcggttcgac ggtataatgt
     2521 cggcgggatc tgtctcttcc agggcgaccc gctcagtcag gccgaccata tcaattttta
     2581 ccaggggatc acccgcacgc ccatcctctt ttgcatcgat gcggagaatg ggttgggtat
     2641 gcggatggac agcgtcgaag ggctgccgcg gcagatgatg ctgggggcga cagggaaccc
     2701 atcgcttatc tatcgctatg gccggcttgt cggcgggcag tgccggcgca tcggtatcca
     2761 ggtgaactat gcgcccgtgg tggatatcaa caacaacccg gacaatcccg tcatcaacga
     2821 ccggtcgttt ggggaagaca agtggaaggt ggcggattac ggggtgcagt atatgcgggg
     2881 gctgcaggat gaaggggtca tggcctgcgc caaacacttt cccgggcacg gtgacgtctc
     2941 ggtcgattcc catttcgacc tgccggtgat cgggaaggat cggcgtcagc tggattcgct
     3001 ggagctgttc cctttccgcg cgctgttcaa ggcgggtgtc ggcagtgtca tgatcgccca
     3061 tttgtatatc ccggcaatcg ataatgagcc aaacagggcc acttccctct cctacaacaa
     3121 tgtgaccaaa ctgctgcgca agcagctgca ttaccagggg atcagcttta c
//
LOCUS       DATGFG010001040         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1544506, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001040 DATGFG010000000
VERSION     DATGFG010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544506"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1392
                     /locus_tag="VKQ52_19765"
     CDS             115..1392
                     /locus_tag="VKQ52_19765"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HLZ89505.1"
                     /translation="MILSEWLQFAPQPRPLKDQEKWHVFLSYRSIDRPWVLNLYDVLV
                     DLGHKVFLDQYVLKPGDHLIRNLQDALDASQAAVLIWSAATKGSVWVNDEYEYLQQRA
                     NKDRDFIFVPVTMDGSELPAFARNRIYVDFSGYPEGPNGGDLLRLLYALANKPLSEEA
                     IQFASDQDEAAAVANALLNNAKRHNKPDRLLQLFHQGGLVWKTSAALGCKTAECLTSL
                     GYNDEAIAVLAEVEKSFPKAIRPIQLHGLALAKRGSSEDLEQAQEILGLLLEKNNADS
                     ETLGIYGRTWMDRYKRTQDETCLRQSRRYYAEAFERQPYDYYTGINSAAKSVFLGEMD
                     KAMEYLQRVETIVGDKPARDDYWKTATVAEVLLIKKQYPAAAAMYRQAVDMAPGQKGS
                     ILSTWGQAALLLESLGAGEKEVAQIRTAFKDYV"
     gene            1385..>2713
                     /locus_tag="VKQ52_19770"
     CDS             1385..>2713
                     /locus_tag="VKQ52_19770"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HLZ89506.1"
                     /translation="MYNDTMEFDLDTFKKRLDSALALFDKEAVNHLCDEFIHYLYGST
                     EPAPARTLELLLQALRNKRMFVPMQKLGDCFIALGLQTCKIRRQYAQSLIDQGIYAAP
                     LAILQELRSETKSDPDPSAANEYAEATGLIGRIYKQQYVNAAGSQNPRRTRLLEQAIA
                     SYQEVYFSNPDLYLWHGINLVALLYRAQSDPIGQAGLPEPAPIALEILSRIEDLDMDR
                     KATAWDYAIACEACIALDRPDESLYHLDKYINCSVADAFEIGSTLRQLQEAWGLTMDS
                     PFGQQLLPLLSAALLRREGATLTISTADLEKHKSSADAHSKDFEQLLSGDSFRTYQWY
                     VKGLKACSAVARIGVDTTLPKGTGFLLDGALLAEEHRGKTLLLTNAHVVSDDSIKNNG
                     SLYVNDAVATLEVVDKDLELKFDGIIRTSNIFELDTTILSFDESAQEKLRS"
BASE COUNT          644 a          824 c          751 g          494 t
ORIGIN      
        1 tcttaccctc taaagtatgg ctcccggatg acgtatgcac cggggaaatc acccattttg
       61 gaaaaagggt gttattcccg tacccgggta gaccaaggat ggctaaatta gggcatgata
      121 ctttccgaat ggttgcaatt cgcccctcag ccaaggccgc tgaaagacca ggaaaaatgg
      181 catgttttcc tgtcctaccg ttccatcgac cggccatggg tattgaactt atacgatgtc
      241 ctggtcgacc tgggccataa ggtcttcctc gaccaatatg tgctgaaacc cggggaccat
      301 ctcatccgga acctgcagga cgccctggac gcaagccagg ccgccgtcct catatggtct
      361 gccgcgacaa aagggtcggt gtgggtaaac gatgaatatg agtacctcca gcaacgggcg
      421 aacaaggaca gggatttcat tttcgtaccg gtgaccatgg atgggtcgga acttcccgcc
      481 tttgcccgca accgcatcta tgtcgacttc tccggctatc ccgaaggccc caacgggggc
      541 gatctgttga gactcttata tgcactggcc aacaagccgc tcagcgaaga agctattcaa
      601 ttcgcctccg accaggacga ggcggcggcc gtcgccaatg ccctcctgaa taacgccaaa
      661 cggcacaaca aacccgaccg cctgctgcaa ctgttccacc agggcggcct ggtgtggaag
      721 acgtccgcgg ccctcggctg caaaaccgcc gaatgcctca ccagcctggg gtataacgac
      781 gaagcgatag ccgttctcgc ggaagtggag aaatcctttc cgaaggccat ccggcctata
      841 cagctgcacg gactcgccct cgccaagcgg ggcagcagcg aggatctgga acaggcgcag
      901 gagatccttg gcctcctgct ggagaagaac aatgccgact ccgaaaccct gggcatctat
      961 ggacgcacct ggatggacag atataaaagg acacaagacg agacctgcct gcggcagtcc
     1021 cgccgctatt acgccgaggc cttcgaacgc cagccatacg attactacac ggggatcaac
     1081 tccgccgcta agagcgtttt tttgggcgag atggacaagg ccatggaata cctgcagcgc
     1141 gtggagacga tcgtcggcga caagccggcc agggacgact actggaaaac agccaccgtg
     1201 gccgaagtgc tgctgataaa aaaacaatat cccgcggcgg cagccatgta ccggcaggca
     1261 gtcgacatgg cgccgggcca gaaaggcagc atcctgtcca cctggggcca ggccgcactc
     1321 ctgctcgaaa gcctgggcgc cggcgaaaaa gaggtggcgc agatcaggac cgcattcaaa
     1381 gattatgtat aacgatacca tggaattcga cctcgacacg ttcaaaaaac ggctcgattc
     1441 ggcattggcc ctttttgata aagaagccgt caaccacctc tgtgatgaat tcattcacta
     1501 tctctacggc tcgacggaac cggcgcccgc caggaccctg gagcttctcc ttcaggcctt
     1561 gcggaacaag cggatgtttg tccctatgca aaagttgggg gactgtttta tcgcgctggg
     1621 tctgcagacc tgcaagatcc gcagacaata tgcccagtcc ctcatcgacc agggcatcta
     1681 tgccgcgccg ctggccatcc tgcaggaact caggagcgaa acgaagtccg accccgaccc
     1741 atcggccgca aacgagtacg cggaggctac cgggctcatc gggcgtatct acaaacaaca
     1801 atatgtcaat gccgccgggt cgcaaaaccc ccggcggacc cgactgctgg agcaggcaat
     1861 agcgtcctac caggaagtct attttagcaa cccggacctc tatctctggc acggcatcaa
     1921 cctggtggcc ctgttgtatc gcgcgcagtc ggaccccatc gggcaggcag gattgcccga
     1981 gcccgcaccc atcgccctgg agatcctgtc gcgcatcgaa gacctggaca tggaccgtaa
     2041 agccacggcc tgggactacg ccatcgcctg tgaagcctgt atcgccctgg accggccgga
     2101 tgaatcgctg taccacctgg acaagtatat caattgctcc gtcgcggacg ccttcgaaat
     2161 tgggagcacc ctgcgacagt tgcaggaagc ctggggactg accatggaca gccctttcgg
     2221 ccagcaactc ctgccgctgc tgagtgccgc cctgctcagg cgggaaggcg caacactgac
     2281 gatcagcacg gccgacctgg agaaacataa atcctcggcc gacgcgcata gcaaagactt
     2341 cgagcagctg ttgagcggcg attccttccg cacctatcaa tggtatgtaa aagggctgaa
     2401 agcctgttcg gcggtggccc gcatcggggt ggataccacc ctacccaagg gcacgggttt
     2461 cctcctggac ggcgccctcc tggcggaaga gcatcgggga aagacgctgc tgctcaccaa
     2521 tgcgcatgtg gtcagcgacg actccatcaa aaataacggc tccctgtacg taaacgatgc
     2581 ggtggctacg ctcgaggtag tggacaaaga ccttgaattg aaattcgacg ggatcatccg
     2641 cacctccaat atcttcgagc tggatacaac catcctgagc ttcgacgaat ccgcccagga
     2701 aaaattgcga tcg
//
LOCUS       DATGFG010001041         4461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_56712, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001041 DATGFG010000000
VERSION     DATGFG010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56712"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /locus_tag="VKQ52_19775"
     CDS             <1..1931
                     /locus_tag="VKQ52_19775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ89507.1"
                     /translation="NFREWWEMNVDIQDLKNAYSRTHQNTTWNWKNVQDETNGLVPIY
                     WNNPYYDRYESYETDGRSRYFGNVYLNYKPTEYLNFLGRVSLDNYSELQQERYAVGSV
                     GVPYYSRFNHTFQEYNYDFLANFDKNISNDFNLKALIGTNLRQDLDNSIYGITNGGLV
                     LPHLFTLSNSINTPNAPYERYTNIQVGGVFAGATLSWRKMLTLDGTVRRDQSSTLPAG
                     HNSYYYPAVSGSFVFSELLKSQNWLSYGKIRANYATVGHSANAYGVYDTYNLNPPFGT
                     NAVASATATKNNIGLVPEKNRSYEFGVEASVLQSRLGFDVTYYHALTYNQIMPVQISD
                     ATGYDARWLNAGSIQNQGFEVSVNGTPVKTHNFSWDITVNWTRNRNKVVALYDTATNL
                     QIAAFQGNVTLNATKGQPYGTIRGNDFIYTNGQRTVSNDPNNNPGYYETTSTSNNVIG
                     NANPDWLGSITNTFRYKNWTLNFLIDARKGGQLFSLDMYYGLATGLYPETAGKNDLGN
                     PVRNSIANGGGIIYKGVTPDGKANTQRVDISDLFGAYGYLRNPAKAFVYDAGFVKLRE
                     AALSYSLPAKVVGKLGQNVFKGIDVSVIGRNLWIIHKNLPYADPEEIVTSGNAALGYQ
                     VGAYPTARTFAFNLKLRF"
     gene            1943..3367
                     /locus_tag="VKQ52_19780"
     CDS             1943..3367
                     /locus_tag="VKQ52_19780"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ89508.1"
                     /translation="MKRVIYFSLSIFVLASCQKNMTSLNINPEAPSVVPSSTLVTQAE
                     FNMASTASSSNVNLNIFRLIQQYWTETTYTDESNYNLATRSIPDNWWNAWYRDVLNNL
                     QQARVFARKDITDAGTLANDTAIIDILQCYAYFNLVTTFGNIPYSQALNPENTFPKYD
                     DAKTVYYDIISRLGKDAAALNPSAGSWGNADILYNGSPAAWAKFAYSLQLKMGALIGD
                     SDPTESKSVMEAAAPHVFASNADNATFSFQSSPPYTNPIWVDLVQSGRTDFVATTTVI
                     GIMTPLNDPRIPNYFTTDANNSYSGGIPGKGNNYPAFSHAGTAIVAPTFPGDLLDYAE
                     TRFYLAEGAARGFNMGAATAEQNYDSAITASIEFWGGTASDAATYLAQPTVAYTTATG
                     TWQQKIGTQLYIALYNRGWDSWTEYRQLNYPTLVAPPTALSVFPQRFLYPSKEENVNT
                     ANYNAASAAIGGDKVGTKLWFDKN"
     gene            3445..3759
                     /locus_tag="VKQ52_19785"
     CDS             3445..3759
                     /locus_tag="VKQ52_19785"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HLZ89509.1"
                     /translation="MADQQTGNNQLNIEISEEVAEGQYANLVIITHSHAEFVIDFVNV
                     MPGTPKSKVKSRMILTPQHAKRFMKALTENIARFEAGNGKIQDLEDVQLPMNFGGPSA
                     QA"
     gene            complement(3887..>4461)
                     /gene="radC"
                     /locus_tag="VKQ52_19790"
     CDS             complement(3887..>4461)
                     /gene="radC"
                     /locus_tag="VKQ52_19790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HLZ89510.1"
                     /translation="NAETNAIDIGREILRVCQDSILELGRRSVKDLMKIKGIGQAKAV
                     TLVAALELGRRWYAREAVHYPIVKDSREVERHLRPLLMHYTYEVFGVLFLAQSGRIIK
                     EFEILSQGGITSTTVDPRLIFRRALELGAVSIVVSHNHPSGSVRPSKADLALTQKLQQ
                     GAKYMDIKLLDHIIIGRDGYYSFADEGLLA"
BASE COUNT         1081 a         1420 c         1056 g          904 t
ORIGIN      
        1 ccaacttccg cgaatggtgg gaaatgaacg tcgacatcca ggacctgaag aacgcgtact
       61 cccggacaca ccagaacacc acctggaact ggaagaacgt gcaggacgag accaacggac
      121 tggttcccat ctattggaac aacccctact acgatcgcta cgaaagctat gagaccgacg
      181 gtcgcagccg gtattttggg aatgtctacc tcaattacaa acccaccgag tacctgaact
      241 tcctcggccg cgtctcgttg gacaactaca gtgaattaca gcaggaacgc tatgcggtcg
      301 gtagcgtcgg ggttccctat tactcccggt tcaaccatac cttccaggag tacaactatg
      361 acttcctggc caatttcgac aagaatatca gcaatgactt caacctgaag gccctgatcg
      421 gtacgaacct ccgccaggac ctcgacaatt ccatatatgg cattaccaat ggcggcctgg
      481 tgctcccgca tctctttacc ctgagcaact cgatcaatac gccgaatgct ccttacgagc
      541 gatacaccaa tatccaggtg ggcggcgtct ttgccggtgc aacgctgagc tggcgcaaaa
      601 tgctcaccct ggacgggacc gttcgtcgcg accagtcctc gaccctgcct gccggccaca
      661 attcctacta ctatcctgcc gtgtcgggca gctttgtctt ctccgaactg ctgaagagcc
      721 agaactggct gtcctacggt aaaatccggg ccaactacgc taccgtgggt catagcgcca
      781 atgcctatgg ggtgtatgat acgtacaacc tgaacccgcc cttcggcacc aacgccgtag
      841 cttccgccac cgctacgaaa aataacatcg gccttgttcc ggaaaagaac agaagctatg
      901 agttcggggt ggaagcatcc gtcctgcaaa gccgcctggg cttcgatgtg acctactatc
      961 acgcccttac gtacaaccag atcatgcctg tacagatctc cgatgccacc ggttatgacg
     1021 cccgctggct gaacgccggt tccatccaga accagggttt tgaagtgtcg gtcaacggca
     1081 cacccgtcaa gacccataat ttctcctggg atatcacggt taactggacc cgtaacagaa
     1141 acaaggtggt tgccctgtac gacacggcta ccaacctgca gatcgcggcc ttccagggta
     1201 acgtgaccct taacgccacc aagggccagc cctatggtac catccgcggc aatgacttca
     1261 tctacaccaa cggccagcgg acggtaagca acgacccgaa caacaatccg ggttattacg
     1321 agacgacctc aacttccaac aacgtgatcg gcaatgccaa ccccgattgg ctgggctcga
     1381 tcacgaatac cttccggtat aagaactgga cgcttaattt cctgatcgat gcacgcaaag
     1441 gcggtcagct gttctcactt gacatgtact acggtctcgc caccggtctg tatcccgaaa
     1501 cagcagggaa gaacgacctg ggcaaccccg tcaggaactc catcgccaac ggtggcggta
     1561 tcatctacaa gggtgtgaca ccggatggca aggcgaatac gcaaagagtg gacatcagcg
     1621 acctcttcgg cgcctatggc tacctgcgga acccggccaa ggcctttgtc tacgacgccg
     1681 gtttcgtaaa gctccgcgag gctgccctca gctattccct gcctgcaaag gtggtcggca
     1741 aactgggaca gaacgtcttc aagggtatcg acgtctcggt catcggccgc aacctctgga
     1801 tcatccacaa gaacctgcct tacgccgatc ccgaagagat cgtcacctcg ggtaacgccg
     1861 cattgggcta ccaggtaggc gcttatccga cggcccgcac ctttgccttc aacctgaaat
     1921 taagattcta aacttattga atatgaaaag agtaatctat ttttcccttt caatattcgt
     1981 actggcctcc tgtcagaaga acatgaccag cctgaatata aatcctgagg cgccgtccgt
     2041 tgtgccctct tccacgctcg tgacgcaagc tgaattcaat atggccagca cggcatccag
     2101 ctccaacgtc aacctcaata tcttccggct gatccagcaa tactggacgg agactacgta
     2161 caccgatgag agcaactata atctggccac ccgcagcatt cccgataact ggtggaatgc
     2221 ctggtaccgc gacgtgctga ataatctgca acaggcaagg gtattcgccc gcaaggatat
     2281 caccgatgcc gggaccctcg ccaatgatac ggccatcatc gatatcctgc aatgctatgc
     2341 ttattttaac ctggtgacca cctttgggaa tatcccgtac agccaggcgc tgaatccgga
     2401 gaacaccttc ccgaagtacg acgatgcaaa gacggtgtac tatgatatca tctcccgcct
     2461 gggcaaggat gccgcagcgc tcaatccctc tgcagggtcc tggggcaatg ccgatatcct
     2521 ctacaatggt tctcccgcgg cctgggcaaa attcgcctac agcctgcaac tgaaaatggg
     2581 agcgctcatc ggcgattcag atcccaccga gtcaaaatcc gtcatggaag cagcggctcc
     2641 ccatgtcttc gcctccaacg ccgataacgc cacgttctcc ttccagagct cgcctcccta
     2701 caccaaccct atctgggttg acctggtgca aagcggccgg accgacttcg ttgcaacgac
     2761 gaccgtcata ggcatcatga ctccgcttaa cgatccccgg atccccaact actttactac
     2821 ggatgccaac aattcctatt ccggcggcat cccgggtaaa gggaacaact accccgcctt
     2881 ctcccacgcc ggcaccgcga ttgtcgcacc gaccttcccc ggtgacctgc tggattatgc
     2941 cgagacccgc ttctatctcg ccgaaggcgc cgccagagga tttaacatgg gcgccgccac
     3001 cgcggaacaa aattatgata gcgctatcac ggcctccatt gaattctggg gtgggactgc
     3061 atccgacgcc gcaacctatc ttgcccagcc caccgtggct tacacgacgg ctaccggcac
     3121 ctggcaacag aagatcggca cgcagctcta catagcgttg tacaaccggg gctgggattc
     3181 ctggaccgaa taccgccagc tgaactatcc gacactggta gctccgccga cggccctctc
     3241 ggtgttcccg caacggtttc tctatccctc caaggaagag aatgtcaaca ccgcgaatta
     3301 caatgccgca tctgccgcca tcggtggcga taaggtgggc accaagctgt ggttcgataa
     3361 gaattaaggg attgcccgaa caggatattt acgcaaaagg acgctcattg agcgtccttt
     3421 tgtattttta ccaaaatata tcgcatggca gaccagcaaa cagggaataa tcaactcaat
     3481 atcgagatca gtgaagaagt agccgaaggt caatatgcca acctggtgat cattactcat
     3541 tcacacgccg aattcgtgat cgatttcgtc aacgtcatgc cgggaacccc caaaagcaag
     3601 gtaaaatccc ggatgatcct cactccccaa cacgccaaac gctttatgaa agccctgacg
     3661 gaaaatatag cccggttcga agccggcaac gggaagatcc aggacctgga agatgtccag
     3721 ctgcccatga actttggcgg cccatccgcc caggcataga cacgctccgc ccgccgcccc
     3781 gccaaaccac ccccagcgcc gcgccaaacc acccggcgaa accaccggcc gccgccccgc
     3841 caaaccaccc aggcttaggc tcacctcgcc aaaccacccc agcgccctag gccagcaacc
     3901 cttcatccgc aaaactgtaa taaccgtccc ggccgatgat gatatggtcc aatagtttga
     3961 tatccatgta tttagccccc tgttggagct tttgggtaag cgccaggtcc gccttactcg
     4021 gacggacgct gccggaagga tgattgtggc tgacgacaat gctcacggcc cccagctcca
     4081 gggcgcgacg aaaaataagc ctcgggtcca ccgtggtgct ggtgatgccc ccctggctga
     4141 ggatctcgaa ttcctttatt atcctccccg attgcgccag aaaaagcacg ccgaaaacct
     4201 cataggtata gtgcatcagc agcggccgca gatgacgttc cacttcccgg ctgtctttaa
     4261 ctatcgggta atgaacagcc tccctcgcat accatctccg ccccagctcc agcgcagcca
     4321 ccagcgtgac ggcctttgcc tgcccgatgc cctttatctt catcaggtct ttcaccgacc
     4381 gccgccccag ttccagtata ctatcctgac agacccgtaa tatttccctg ccgatgtcga
     4441 tggcattggt ttccgcatta c
//
LOCUS       DATGFG010001042         5714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_857468, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001042 DATGFG010000000
VERSION     DATGFG010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857468"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VKQ52_19795"
     CDS             complement(<1..310)
                     /locus_tag="VKQ52_19795"
                     /inference="COORDINATES: protein motif:HMM:NF025048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89511.1"
                     /translation="MNTAVSREPFPASRKIYVPGKAHALQVAMREIQTGDGPVTVYDT
                     SGPYTDPAVGIDLERGLPRVREAWVKERRGGGNVTQMHYARKGIITPEMEYIAIRENQ
                     R"
     gene            complement(344..550)
                     /gene="thiS"
                     /locus_tag="VKQ52_19800"
     CDS             complement(344..550)
                     /gene="thiS"
                     /locus_tag="VKQ52_19800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HLZ89512.1"
                     /translation="MPEVSINQQKFQLPEDGRLADVLPLLQIGQADGIAMAVNETVIP
                     RSEWAGYRLQEADRVFVIRATQGG"
     regulatory      complement(591..686)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(718..2067)
                     /locus_tag="VKQ52_19805"
     CDS             complement(718..2067)
                     /locus_tag="VKQ52_19805"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HLZ89513.1"
                     /translation="MRYFPKWLLLLLVVTGVRAQVAVKPPMGWNSYNCFGSAVHEDEV
                     KANADYMAQNLARYGWQYIVVDFLWSYDNPPGSLIGNPFQLRLQDGSYVPWLAMDEWG
                     RLQPHVDKFPSAFGGKGFKPLADYVHSLGLKFGIHVMRGIPRQAVWAKSRVMGASGVT
                     ADAIADTLSKCPWMNHMYGLNMQKPGAQEYLNSLLSLYADWGVDLIKVDDISRPYSAA
                     EIEGYRKAITACGRPIVLSLSPGETPLADSGHVKTFGNMWRMADDFWDSWPEMLKMFD
                     YARKWEGVGGAGHWPDCDMLQIGKVSKRGPVGPERYSRFTEDELLTHLTFWCIYRSPL
                     MLGGNLPENRDIEKRLFTNGEVMGVNQEGTNPRQVIKSEGKMVWVSDAGGGAMYVGLF
                     NISDAAQAVAVDLAGLGIRRAVAVRDLWQQQDIGIYKKQYQRMLPAHGCALLKIVPR"
     gene            complement(2077..3597)
                     /locus_tag="VKQ52_19810"
     CDS             complement(2077..3597)
                     /locus_tag="VKQ52_19810"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HLZ89514.1"
                     /translation="MNATRTLLSLTLVLFSFSLFAQNGGGLTIRDTSGPVISRNLYGH
                     FSEDLGRCIYDGFWTGDHIRMDVVDALKKIRIPVLRWPGGCYADQYHWRDAIGPRDQR
                     KHTVNTTWGMVDEDNSFGTHEFLQLCDLLGCQPYIAGNVGTGTPEEMENWLEYLNYNG
                     KSTLANLRRQNGREQPWNVSFWGVGNESWGCGGSMTPEYYSGEYNRYSEFCKNYPGTK
                     LKLIASGANSDDYNWTETVMKNIDRGRTWGLSMHYYTLTGSWEHKGSATQFTEDEYFR
                     AMVSCLRMETIVSRHAAIMDKYDPRKRMALVVDEWGIWTDAEPGTNPAFLYQQNSLRD
                     ALIAATTLNIFNNHADRVKMANLAQTVNVLQALILTDKDRMLLTPTYHVFDLYKVHQD
                     ARSLVMHLWSPEYVHGAEKIPAVNASASRDSSGVVHISLVNLDPAKKVAVRANLPGIT
                     FKTVQGQVLTSARFTDVNTFDDPNRVKPAAFAGAKKDGADLVVELPAMSVVVLELK"
     gene            complement(3703..4953)
                     /locus_tag="VKQ52_19815"
     CDS             complement(3703..4953)
                     /locus_tag="VKQ52_19815"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HLZ89515.1"
                     /translation="MSTKSIQPLIRKTHRAAVDLRRASDRQVKTALQLLAKSLEEESA
                     ALLRANARDLSRQEPDNPRNDRLLLNEKRIKAIAQSIRKVGRLPDPSGRLLESRTLDN
                     GLKVEKRAVPLGVVGAIYESRPNVTFDIAALCLRSRNACLLKGSQEAAETNRAAMVLI
                     KKALAAAGIPPDCVTLLPPDREVVQELFTATRWVDVLIPRGSDALIKFVRANSLVPVI
                     ETGAGVCHVYVEKEADLDKAAAIVVNAKVSRPSVCNAMDTLLVDRVVAPGLLKRLRAP
                     LEKHGVELFADREAAGLLAGYPYLQRAKPEDYAREFLSLKCAVKVVKDIDEALEHIDR
                     YSTKHSEAIVSRNKQQCERFLAEVDAAAVYANASTRFTDGEALGLGAEIGISTQKLHA
                     RGPFALEKLVTEKWVIRGTGQIRE"
     gene            complement(4955..>5714)
                     /locus_tag="VKQ52_19820"
     CDS             complement(4955..>5714)
                     /locus_tag="VKQ52_19820"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HLZ89516.1"
                     /translation="PVAQALCERQHFSSRPRFLQLKETFAEFWKNGILPVMNENDLVS
                     NVELKFSDNDELATLTAIGFDAEALLLCTSVGGFMDADRKIIPVVEKVDARIMGFVQE
                     GKSSLGLGGMLSKLTFTRLATSLGIRVVICGLGGNNPFADALAGKAGTSFMTRVSNLR
                     ARQKWLASGSITLGSLYLDKGAIRALEQRHSLLSVGIRRVQGKFAAGEVVQLKDEEEQ
                     IVGVAKVKLGVSEMTARLMNKNTIAAHADDIVLF"
BASE COUNT         1267 a         1952 c         1320 g         1175 t
ORIGIN      
        1 cccgctgatt ctcgcggata gcgatatact ccatttccgg ggtaatgatc cccttccgag
       61 cataatgcat ctgcgtcaca ttcccgccac ccctcctttc tttcacccat gcctcccgca
      121 cccgcgggag gccccgttcc agatcgatcc cgacagcggg atcggtatag ggtccgctcg
      181 tatcatacac cgtcaccggc ccgtcaccgg tctgtatctc ccgcatggcc acctgtaagg
      241 catgtgcctt cccgggaaca tagatcttcc gcgacgcggg aaagggctcc cggcttactg
      301 ctgtattcat atagataatt ttttcaccca cgccggggca gccttaccca ccctgcgtcg
      361 ccctgatcac aaaaacccgg tccgcttcct gcagccgata ccccgcccac tcggaacgcg
      421 gtatcaccgt ctcattaacg gccatggcta tcccatccgc ctgcccgatc tgcaaaagcg
      481 gtaacacatc ggccaaccgg ccgtcctcgg gcaactgaaa tttttgttga ttgatcgaca
      541 cctccggcat aacgtataca ataaaaaaag aggtgctgcg tcgcgcaaaa agagaaagtc
      601 cgtcttttcc cttcgccggt attacccgga tcaggttttg agggtatcat ctcagcccgt
      661 gtgaacgagc acccctaaag tacaggccaa aggtagggaa ataaaataca ccgctcgcta
      721 acgcggaaca atttttaaaa gcgcgcatcc atgcgccggc aacatccgct ggtactgctt
      781 cttgtatatg ccgatatcct gctgctgcca aagatcacgc accgcaacgg cacgccggat
      841 acccaaaccg gcaagatcga cagccaccgc ctgcgcggcg tcgctgatat tgaacaaccc
      901 cacatacatc gcgccgccac cggcatccga cacccagacc atcttgcctt ccgacttgat
      961 cacctggcgg ggattcgtcc cttcctggtt gacacccatc acttccccat tggtgaacaa
     1021 ccgcttctcg atatcccggt tctccggcag attacccccg agcatgagcg gcgaccggta
     1081 gatacaccag aaggtcaagt gcgtgagcaa ctcgtcttcg gtgaaccggc tgtatcgctc
     1141 cggaccgacc ggtcctcgtt tggagacctt cccgatctgc aacatatcac aatccggcca
     1201 gtgacccgca ccgccgacac cttcccactt ccgggcatag tcgaacatct tcagcatctc
     1261 cggccagcta tcccagaaat catccgccat ccgccacata ttcccaaagg tctttacatg
     1321 cccggagtcg gctaacggcg tctctcccgg agaaagacta agaacgatcg gccttccaca
     1381 ggcggtgatc gccttccggt aaccctcgat ctccgccgca ctgtacggcc gggaaatatc
     1441 atccaccttg ataagatcca caccccaatc cgcatagagc gaaagaaggg agttgagata
     1501 ttcctgcgcc ccgggctttt gcatattcaa cccatacata tgattcatcc agggacattt
     1561 cgataaagta tcggcaatgg catcggcggt aaccccactc gcccccatca ccctgctctt
     1621 cgcccacacc gcctgacggg gaataccccg catgacatgg atgccgaatt tcaaccccag
     1681 cgaatgaaca taatccgcaa gcggcttaaa ccccttgccg ccaaacgccg aggggaattt
     1741 atcgacatgc ggctgcagcc gtccccattc atccatcgcc agccagggaa catagctccc
     1801 gtcctgcaac cgcagctgaa aaggattccc gatcaggctg ccgggcgggt tgtcatatga
     1861 ccagagaaaa tccacgacga tatactgcca gccatagcgg gccaggttct gcgccatata
     1921 atcggcattg gccttcacct catcctcatg caccgccgag ccaaaacagt tatagctgtt
     1981 ccagcccatc ggcggcttca ccgccacctg cgcacgcacc ccggtcacaa ccagcaataa
     2041 caacagccat ttcggaaaat atcgcattgg gtctccttat ttcaattcca gcacaaccac
     2101 cgacatggcc ggcaattcca caacaagatc ggcgccatcc ttcttcgcac cggcaaaagc
     2161 agccggcttc acccggttgg gatcgtcaaa cgtattgaca tcggtgaatc gcgccgaggt
     2221 aagcacctgt ccctgcaccg tcttgaaagt gatacccggc agattggcgc ggacagcgac
     2281 ctttttcgcc gggtccaggt tgacaaggga aatatgcacc accccgctgc tgtctcttga
     2341 agccgaagca ttgacggccg ggatcttctc cgccccatgc acatactccg gactccacaa
     2401 atgcatcacc agcgaacggg catcctggtg aaccttgtac agatcgaata catgataggt
     2461 aggcgtaagc aacatccggt ccttatccgt aaggatcagc gcctgcagga cattgaccgt
     2521 ctgcgccagg ttggccatct tcacccggtc cgcatggtta ttgaaaatat tcagggtcgt
     2581 ggcggcgata agcgcatcac gcaggctgtt ctgctgataa aggaaagcgg gattggtgcc
     2641 cggctccgcg tccgtccaga taccccattc atctacaaca agcgccatcc gcttgcgggg
     2701 atcgtacttg tccataatgg ccgcatggcg gctcacgatc gtttccatcc gcaaacagga
     2761 aaccatcgcc cggaaatatt cgtcctccgt gaactgcgtg gcagagccct tgtgctccca
     2821 actccctgtg agtgtataat agtgcatcga gagaccccag gtgcgaccac gatcgatatt
     2881 cttcatcacc gtctcggtcc agttgtagtc gtcgctgttc gccccactgg cgataagctt
     2941 caacttagta ccggggtagt ttttgcaaaa ctccgaatag cggttgtact cgccggaata
     3001 atactccggc gtcatgctgc cgccacaacc ccagctttca ttacccaccc cccagaagga
     3061 aacattccag ggctgctccc ggccattctg ccggcgcagg ttcgccagcg tgctcttgcc
     3121 gttatagttc aggtattcca gccagttctc catctcctcg ggcgtacccg tacccacatt
     3181 ccccgcgata tacggctggc agcccagcag atcgcagagc tgtagaaatt catgcgtgcc
     3241 aaaactgtta tcctcatcga ccatccccca ggtggtgttc accgtatgct ttcgctggtc
     3301 ccgcggtccg atggcgtccc gccagtgata ttgatccgca tagcaacccc ccggccagcg
     3361 cagtaccggg atacggatct tcttaagcgc atccaccaca tccatccgga tatgatcacc
     3421 cgtccagaaa ccatcataga tacatcgccc gagatcctcg gaaaaatgcc catacaggtt
     3481 acggctgatc accgggcccg aggtatctcg tatcgtcagg ccgccgccgt tttgcgcgaa
     3541 cagggaaaaa gaaaagagaa caagagtcag agagagaagg gtcctggtag cattcatatg
     3601 ctttaatcgt tttaggagcc ctaaattaag cgttttcttg acaattaata aaaattattc
     3661 ggccgccgcg cctccggcca ggagccaccc cgggacaaaa tcctactccc ggatctgccc
     3721 cgtcccccgg atcacccatt tctccgtcac cagcttctcc aatgcaaagg gcccgcgggc
     3781 atgcagcttc tgcgtggata tcccgatctc cgccccaagc ccaagcgcct ccccatcggt
     3841 aaaccgggta gaagcattcg catacaccgc cgccgcatcc acttccgcga gaaaccgctc
     3901 acactgctgt ttattccgcg agacaatagc ctccgaatgt ttcgtcgaat agcgatcgat
     3961 atgctccagc gcctcgtcga tgtccttcac cacctttacc gcacacttca ggctaaggaa
     4021 ctcccgggca taatcctccg gctttgcccg ctgcaaatac ggataccccg ccagcagccc
     4081 ggccgcctcc ctgtccgcaa acaactcaac gccatgcttt tcaagcggcg cccgcaaccg
     4141 cttcagtaaa cctggcgcca ccaccctgtc cacaagcagc gtgtccattg cattgcagac
     4201 cgacggcctc gataccttgg cattgacgac gatcgccgcc gccttatcca ggtcggcctc
     4261 cttctccaca taaacatgac agacccccgc ccccgtttcg atcacaggca caagactatt
     4321 ggccctcaca aactttatca gcgcatcgga accccgcggg atcagcacat cgacccagcg
     4381 cgtggcggtg aacagttcct gcaccacctc ccggtccggc ggcaaaaggg tcacacagtc
     4441 gggagggata cccgccgccg ccagggcctt tttgatcagc accatcgccg cccggttggt
     4501 ctcggccgcc tcctggctgc ccttgagcag acaggcatta cggcttcgca gacaaagcgc
     4561 cgcaatatcg aaggtgacat tgggcctcga ctcatagatc gctcccacca cccccagcgg
     4621 caccgcccgc ttctccacct ttagcccgtt atccagcgtc ctgctttcca gcaaccgccc
     4681 cgaggggtca ggcaacctgc ccaccttacg gatactctgc gcaatggcct ttattcgttt
     4741 ctcattcaac aacagccggt cattacgcgg gttgtccggc tcctgccgcg aaaggtccct
     4801 ggcattggcg cgtaaaagcg cggcgctttc ttcttccagg ctcttcgcca gcaactgcaa
     4861 cgccgtcttc acctgccggt cactggcccg tctcagatcc accgccgcac gatgcgtctt
     4921 ccggatcagc ggctgtatac tctttgtgct catattaaaa tagtacgata tcgtccgcat
     4981 gcgcggcgat cgtgttcttg ttcatcaatc tggcggtcat ctccgacacc cccagcttca
     5041 ccttcgccac ccccacgatc tgctcctcct cgtctttcag ctgtaccacc tcgcccgccg
     5101 caaactttcc ctgcacccgc cggataccca cactaagcag gctatgccgc tgttccaggg
     5161 cccggatggc ccccttgtcc aggtagaggc tccccagggt gatcgatcca ctggccagcc
     5221 atttctgccg ggccctgaga ttggaaaccc tggtcataaa actcgttccc gccttccccg
     5281 ccagcgcatc cgcaaaagga ttattgccgc caagcccaca gatcactacc cggatcccaa
     5341 gggaagtggc cagccgcgta aaggtcagct tcgataacat cccccccaat cccaggctgc
     5401 tcttcccctc ctggacaaaa cccatgatcc gggcatccac cttctcaacg acagggatga
     5461 tcttccggtc ggcatccata aacccaccca ccgaggtgca aagcaaaagc gcctccgcat
     5521 cgaatccgat ggcggtaagc gtagccaatt catcattgtc ggaaaatttc agctctacat
     5581 tgctgacaag atcgttctcg ttcataaccg gcagaatgcc gttcttccag aactcggcaa
     5641 aggtctcctt cagttgcagg aagcgcggcc ggctcgaaaa atgctgccgc tcgcacaacg
     5701 cctgcgccac cggt
//
LOCUS       DATGFG010001043         7316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_580238, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001043 DATGFG010000000
VERSION     DATGFG010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580238"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VKQ52_19825"
     CDS             complement(<1..736)
                     /locus_tag="VKQ52_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89517.1"
                     /translation="MKGVAQNPGFRIPLQLSDTTRKYDDSTLRIRNINPYFTVHVDSV
                     LSYQFEVNRDPSRYYFFLRNAPVGLKIRENGLLTFKADKSYFLSGRLKYDYEYHVTVG
                     VQNLNDPKDRIDTSFIISFFTTEIIPSHVKPTVSNVLYMEEGDTISFRVQCETGTYPI
                     ESINFYSNIPIKTNSVIKGCNDDFTWSPPYDFVKDNDSAKQKLLLLYFVGTNKVFMRD
                     TATIRIYVRDALNYPYMVEQYNSAVKN"
     gene            complement(1053..1484)
                     /locus_tag="VKQ52_19830"
     CDS             complement(1053..1484)
                     /locus_tag="VKQ52_19830"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLZ89518.1"
                     /translation="MTLVKVNNNGHRSLSNFVDEFFQGLPSGLGREESVGFPPVNIHE
                     TADAYHLELSAPGRSKEDFKLSVDNGQLTVGFEKKEETKTEEYKTIRKEFSFKSFKRS
                     FNLDDRIDSNAIQAKYENGVLKLFLPKKEQVKESTKQITIQ"
     gene            1709..2122
                     /locus_tag="VKQ52_19835"
     CDS             1709..2122
                     /locus_tag="VKQ52_19835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HLZ89519.1"
                     /translation="MYPREIVLPMKEELTDNGFHELLSSGEVDSQLQKAGTTLVMVNS
                     VCGCSAGSARPGVLMAVANSSKKPDFLATSFAGFDLEAVNKIREHLLPYPPSSPAIAL
                     FKDGKLVHMIERHQIEGRPAQVIAHNLIAAFDEYC"
     gene            2602..3912
                     /locus_tag="VKQ52_19840"
     CDS             2602..3912
                     /locus_tag="VKQ52_19840"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HLZ89520.1"
                     /translation="MYPNLYFALKDLFGISLPFLRFINSFGFFVALSFNAAAVTLTRE
                     LMRKEGEGLLQYREELVWIGKPASPTELTLNFILGFLLGFKLVGLFTADSALTANVQD
                     YLLSLQGNLPAGLAGGLLFAGLKFYEKNKSKLPQPQEKKVRIWPHDRVGDLTIYAFIF
                     GFAGAKVFNSLETWNDFVRDPIGSLFSFSGLTFYGGLICAALAIWYYARKNGIGFWHL
                     NDAAAPGLMLAYAIGRIGCQVSGDGDWGILNSAYITSSTGKPVLATHDMVLTTLRQNS
                     QVYLPQFGSLDKVPQLSVKAPSFLPDWLFAYSYPHNVIGEGAHIMDCNTQYCSQLPIP
                     VFPTPLYETLTCLCLFFILWRLRKRLHIPGTLFAVYLILNGIERFLVEHIRVNSTYSI
                     FGFHPTQAELISAGLVIGGALLWFNLKRRGAPPAPPATAATSAG"
     gene            4263..6731
                     /locus_tag="VKQ52_19845"
     CDS             4263..6731
                     /locus_tag="VKQ52_19845"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ89521.1"
                     /translation="MRNTFALLTAFVLLFSLPVLAQNTGKVAGAARDAKGQPLESATI
                     TLLHAGDSAKLRVTATDKTGQFRFDHVPAGNYLVAASSVGFSQVYSPTFNLSNEKPSQ
                     NLPPLMLTAAAASLKEFAVVAKRPFLEQKADRMVVNVDASPSNAGSTAMDVLEKSPGV
                     TLDKDDNISLKGKQGVTIMIDGKPTYLTASQLASYLRSLPASAIDQIELMTNPSAKYD
                     AAGNSGIINIKTKKNKLKGFNGNLNLTHTQGVYPKPSGSLNLNYRTGKANFFLNAGYA
                     HWEGFQHLSITRKYLDADKQTINSIFSQETEMHFVNPEMNLKLGMDYYLTPKTTIGFV
                     VSGFRNKEENRSGSNIRLLDPHYALDSVVYSPSTNTTTWKNGSVNLNFRHTYDSSGRE
                     LTADLDYVRYSAISDQYFDNLTYASNMTKLGETVLTGNLPSAINIYTFKTDYTRPLAK
                     GYKLETGIKLSYVNTDNTANYFNVLNNVPEVDTTKTNHFLYKENINAVYINMTKQYKK
                     LGVQLGLRAENTNYSGHQLGNGLSVINRDSSFSRSYINLFPTAYLSYTINDKNQLSLN
                     YGRRIDRPAYQDLNPFLFFLDQYTYQAGNPYLQPQYTNNVELSHTWRHMLTTTLNYSY
                     TSDFFSETFEQSGQATIVRNGNIGSRQNAGVAVSAQLSIAKWWTSILYTNLNYSKFSG
                     MLYGENISVDATTLLLNVNNQFTFPGGWSGEVSGFYRTKGVEGQVVIYPLGQASAAVS
                     KKILHDKASVKLAMRDIFYTNHPHGYINFQQTEATFSNQRDSRQVACTFTWNFGKPFK
                     GVGSRRNSGAGEEENRVKSGGNGN"
     gene            6823..7314
                     /locus_tag="VKQ52_19850"
     CDS             6823..7314
                     /locus_tag="VKQ52_19850"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HLZ89522.1"
                     /translation="MPSFDIVSKVDQQVLDNAVNVTKKEITNRFDFKDSPVKIELNKK
                     DMTIDLEAESEMKIKQIVDVLISRSMKQGLDPLAFDLEKEPYQSGKVTRQEVPVRNGL
                     KQEDAKKVTKLIKDSGLKVQSQIMDDLIRVTGKKIDDLQAVIKLCSGANLGIALQYIN
                     MRS"
BASE COUNT         1767 a         2038 c         1787 g         1724 t
ORIGIN      
        1 cattcttgac ggcggagttg tactgctcca ccatataggg atagttaagc gcatccctga
       61 catagatgcg gatggtggcg gtatcccgca taaatacctt gttggtgccc acaaaataga
      121 gcaggagcag cttttgtttg gcggaatcgt tgtcttttac gaaatcgtag ggcgggctcc
      181 aggtgaaatc gtcgttacat ccttttatga ccgaattcgt cttgatgggg atattcgagt
      241 agaaattgat gctttcgata gggtaggtgc cggtctcgca ttgcacccgg aagctgatgg
      301 tatcgccttc ttccatatac aggacgttac taacggtggg ctttacatgg gaggggatga
      361 tctcggtggt aaagaagctg atgataaagc tggtatcgat ccggtccttg ggatcgttca
      421 gattctggac acctaccgtg acatggtact cataatcgta cttcagcctg ccggagagga
      481 aataggactt gtccgccttg aaggtcagca ggccgttctc cctgatcttc aggccgacgg
      541 gggcattccg taaaaagaag taataccggc tgggatcccg gttgacctcg aactggtagc
      601 tgaggacgga atcgacatgc accgtgaaat aagggtttat gttcctgatc cgtaaggtgg
      661 aatcatcata cttcctggtg gtgtcggaca attgcagtgg aatacgaaat cccggattct
      721 gggcaacccc tttcatacaa aacaacatcc cgaccgatac gaatagccaa agcctaaaga
      781 cgcgcgaagg tttaatcata ttcttggata aaatgggtta tttatgtgcg cagatagttc
      841 tcccggcaaa aatagcccta tccgcgcaaa gagctaaagt ctgaccttcg gtcgggccgc
      901 tgaagcggct ccgggaagcc cgaaattaaa acaaaccatc aaaaaaagcg caaaaaagaa
      961 aggaaacaaa aagcccggcc aaaaggccag gctcttgtta ttcaaccaaa accaaccaat
     1021 tatgaaaaaa acgccctatc ctaattactt tattactgga tcgtgatctg tttggtgctt
     1081 tctttcacct gttccttctt gggcaggaag agtttcagca ccccgttctc gtactttgcc
     1141 tggatggcat tgctgtcgat ccggtcgtcg aggttgaagc tgcgtttgaa gctcttgaag
     1201 gaaaattctt tgcggatggt tttgtactct tctgttttgg tttcttcctt cttttcgaaa
     1261 ccgaccgtca gctgtccatt gtccacagac agtttgaagt cttccttgct gcgaccggga
     1321 gcgctaagct caagatgata ggcgtcagcc gtttcatgga tgttcaccgg gggaaaacca
     1381 acgctttctt cacggccgag accggaggga agaccctgga agaactcatc tacaaaattt
     1441 gaaaggcttc tgtgtccgtt gttgtttact tttacgagtg tcatggctaa tccgtattta
     1501 agtgatgtaa ttttgtttta cgggaaggat ggatcaaacg acataccatc cggccaaaac
     1561 cgctctttaa ccgaagtttt ggcggtgtcg gggtccgatc aaatgacaga atttcatctc
     1621 cgttcccttt ttaatgacat tatttcattg aactttttac ttatttttgt gttttcatag
     1681 ttatcatctt ttaaatattc atagtacaat gtatccaagg gaaatagtat taccgatgaa
     1741 ggaggaactg acggacaatg gattccatga gctgctcagt tccggcgagg tcgattctca
     1801 gttacagaag gccggcacca ccctggtgat ggtcaattcc gtttgcggct gttcggccgg
     1861 ttccgcccgc cccggggtcc tgatggccgt tgccaacagt tccaagaaac ccgattttct
     1921 ggctaccagc ttcgcagggt tcgatctgga ggctgtgaac aagatccggg agcatttgct
     1981 gccgtatcct ccttcttctc ccgctatcgc cctgttcaag gacggaaagc tggtgcatat
     2041 gatcgagcgt caccagatcg aaggccggcc ggcgcaagtg atcgcgcaca acctgatcgc
     2101 cgcttttgac gagtactgtt gatccgatct tattcttaat ggtgtgaccc ttcccggatg
     2161 cggggagggt tttttgtttt tcggtgggga taaacctcac tatttacaat tctattcaca
     2221 tctgtggata agctgcgaaa tgcagatttg gtgggaattt ggcgggaaaa gtgttccccg
     2281 tggaacattg atgtggaacg gcaatcaaca acctttagta tctgtggaaa aaatattctt
     2341 ggagaaaaga tggaaagccg ctataactcg cttaggctcc gggattttga ttgggggaaa
     2401 gaacccgcga tgccgggggc atcggtccga aggacggaca ttagatcgtt ttttgcctga
     2461 ggcgcgctaa atttttccga gcctattgac ggcaaaaaaa attcggtcac attgccgcgg
     2521 caaaaccgta ggtttgtgcg cggagcggat catccccgct gcacaaagca tacaatagct
     2581 gcacataagc atacaataac tatgtatccc aatctttatt ttgctcttaa ggatctgttt
     2641 ggaatttcac tgccttttct tcgttttatc aactctttcg gcttctttgt cgccctttcc
     2701 tttaacgccg ccgccgtcac ccttacccgg gaactaatgc gcaaggaggg ggaggggttg
     2761 ttacaatacc gggaagagct ggtctggatc gggaaacccg ccagccctac cgagctcacc
     2821 ctcaatttta tccttggatt tctgctgggc ttcaagctgg tagggctttt tacggcggac
     2881 agcgccctca ccgccaatgt gcaggattac ctcttatccc tccaggggaa cctccctgcc
     2941 gggcttgccg ggggacttct ttttgccggg ctgaaattct atgagaagaa taaaagtaaa
     3001 cttccccagc cgcaggagaa gaaggtgcgc atctggcccc atgaccgggt tggcgacctg
     3061 accatctatg cctttatctt tggttttgcg ggcgccaagg tctttaacag cctggaaacc
     3121 tggaacgatt tcgtccggga tcccatcggt tcgctctttt ccttcagtgg cctcaccttt
     3181 tatggcggcc tgatctgcgc tgccctcgcc atctggtact atgcccggaa gaatggtatc
     3241 ggcttctggc atctgaacga tgccgcggct cccggcctca tgctggccta tgccatcggc
     3301 cgtatcggct gccaggtctc gggggacggg gattggggga tcctcaatag cgcctatatc
     3361 acttcatcaa ccgggaaacc cgttctggcc acccatgaca tggtgctgac gacccttcgc
     3421 cagaacagcc aggtctatct gccgcagttc ggcagcctgg acaaggtgcc gcagttgtct
     3481 gtgaaagccc cttccttcct gcccgactgg ctttttgcct attcctatcc ccataacgtg
     3541 atcggggagg gggcgcatat catggattgc aatacccagt attgcagcca gttgcccatc
     3601 cccgtcttcc ctacaccgct gtatgagacc ctcacctgtc tctgcctctt ttttatcctc
     3661 tggcgcctcc gcaaacgtct ccatatcccc gggacgctct ttgccgtcta cctgatcctc
     3721 aacggtatcg aacggttcct ggtcgagcat atccgcgtca actccactta tagcatcttt
     3781 ggttttcatc ccacccaggc ggaactcatc tccgcgggac tggtcatcgg cggcgccctc
     3841 ctttggttca acctgaaacg ccgcggcgcg ccacccgccc cgccggcgac ggcagccacg
     3901 tccgccggat aatggtgttc gccatccttc cgtaaatccc tccattcatc actatggcgg
     3961 taacatctgt aacggctgtc cgtctgacag cgtcatagac ctgtccatcg gttcacgatc
     4021 gtccgatggt caccgaaaac gggtcccagg tcaagaaata ttcctgatct cctggcctcc
     4081 gggtagtttt gccctgtaac aggtcgcttt cccgaatccc acatccctta tcattacgaa
     4141 cccattgcca attatcaccg gaatcggcct cccaacacaa aacaagacac catgcaatac
     4201 ataatactaa tctttgtatt gtaatgttta aaatacagta ctaaatatac aacgcttcat
     4261 ttatgagaaa cactttcgcc cttctgactg cctttgttct cttgttctcg ctgcccgtcc
     4321 tggcgcagaa cacaggaaaa gtagccggcg ccgcccgcga cgccaaaggg caaccccttg
     4381 agtctgctac gattacctta ttacatgctg gggactccgc aaaattgcgg gtaacagcta
     4441 ccgataagac cggtcaattc aggtttgatc atgtacccgc cgggaactac ctggtcgccg
     4501 cctcctcggt cggctttagc caggtctatt cccccacctt caacctctcg aatgagaagc
     4561 cgagccaaaa cctgcctccg ctgatgctca ccgccgctgc tgccagcctc aaggaatttg
     4621 ccgtggtcgc caaacggccc tttctggagc aaaaggccga ccggatggtc gtcaacgtag
     4681 acgcttctcc ttccaacgca ggttccaccg ccatggacgt gctggagaaa tcgcccggcg
     4741 tcaccctgga caaggacgat aatatcagcc tgaaaggcaa acagggggtt accattatga
     4801 tcgatggcaa gcccacctat cttacggcca gccaactggc gagctatctc cggagcctgc
     4861 ccgcctccgc gatcgaccag atcgaactca tgaccaaccc ctccgccaaa tacgacgccg
     4921 ccggcaattc cgggatcatc aatatcaaga caaaaaagaa caagctgaag gggttcaatg
     4981 gtaacctcaa cctgacccat acccagggcg tctaccccaa acccagcggc agcctgaacc
     5041 tcaactatcg caccggtaag gccaacttct tcctgaacgc cggttatgcc cactgggaag
     5101 ggttccagca tctgtccatc acccgtaaat acctggatgc ggataaacaa acgatcaact
     5161 cgatcttttc acaggagacc gagatgcatt tcgtcaatcc cgagatgaat ttgaaactgg
     5221 ggatggatta ttacctgaca cccaaaacga ccatcggttt tgtcgtaagc gggttccgca
     5281 ataaggaaga gaaccgaagc ggcagcaata tccggttgct cgacccgcac tatgccctgg
     5341 attcggtggt ctattcgccc agcacgaaca ccaccacctg gaagaacggc agcgtaaacc
     5401 tgaacttccg ccatacctat gactcctccg gccgggaact gacggccgat ctggactatg
     5461 tccgctatag cgccatcagc gatcagtatt tcgataacct cacctatgct tccaatatga
     5521 caaaactggg tgagacggtg ctcaccggca acctgcccag cgcgatcaat atctacacgt
     5581 tcaaaacgga ttatacccgc cccctggcaa aaggatataa actggagacc ggcatcaagc
     5641 tcagctatgt caacacggac aataccgcca attacttcaa cgtgctgaac aatgtccccg
     5701 aagtggatac caccaagacc aaccacttcc tttacaagga gaatatcaat gcggtctata
     5761 tcaatatgac caaacaatac aagaagctgg gcgtacagct gggcctgcgc gcggagaata
     5821 ccaactattc cggacatcag ctgggcaacg gtctgtcggt gatcaaccgg gactcctcct
     5881 tctcccgcag ctatatcaac ctgttcccga ccgcctacct cagctatacc atcaatgata
     5941 agaaccagct gagcctgaat tacggccgcc ggatcgaccg gcccgcttac caggacctga
     6001 accccttcct gttctttctc gaccagtata cctaccaggc cgggaacccc tatctccagc
     6061 cccagtatac caacaatgtc gagctctccc acacctggcg ccatatgctg acgacgaccc
     6121 tgaactatag ctatacctcg gatttcttct cggaaacctt tgaacagtcg ggccaggcca
     6181 ccatcgtccg caacgggaat atcggcagcc gccagaacgc cggggttgcg gtgagcgccc
     6241 agctctccat cgcgaaatgg tggacgtcga tcctatatac caacctgaat tacagtaagt
     6301 tcagcggtat gctgtacggt gagaacatca gcgtggacgc cacgacgctg ctgctcaacg
     6361 taaacaacca gtttaccttc cccggcggct ggagcggcga ggtctccggc ttctaccgga
     6421 caaagggagt ggaaggacag gtggtgatct atccccttgg ccaggcttct gccgcggtgt
     6481 ccaaaaagat cctgcatgat aaggcctcgg tcaagctggc catgcgcgat atcttctata
     6541 ccaatcaccc ccatggctat atcaatttcc agcaaacaga ggctaccttc agcaaccagc
     6601 gggatagccg ccaggtggcc tgcaccttca cctggaattt tggcaagccg ttcaaaggcg
     6661 tcggcagcag aaggaatagc ggcgccggcg aggaggagaa ccgggtcaag tcgggcggca
     6721 acggcaattg atcgctgatg cccttatcgc cgatgcccgg aaaaaagggt gggcctccgg
     6781 tccacccttt ttttaatata tttgccgctc aactccatcg ttatgccatc ctttgatatc
     6841 gtaagcaaag tcgatcagca agtgcttgat aatgcggtca acgtcacgaa aaaagagatc
     6901 accaaccggt ttgatttcaa ggactctccc gtgaagatcg aattgaacaa aaaggatatg
     6961 accatcgacc tcgaagcgga gagcgagatg aaaatcaaac agatagttga cgtcctgatc
     7021 agccggagca tgaaacaggg actcgacccg ctggccttcg acctggaaaa ggaaccctat
     7081 cagagcggga aggtcacccg gcaggaggtg cccgtccgca acggcctcaa acaggaagac
     7141 gcgaagaaag tgacaaagct catcaaggac tcgggactca aggttcaatc ccagatcatg
     7201 gacgatctga tcagggtcac cggcaaaaag atcgacgacc tccaggccgt catcaagctc
     7261 tgcagcggcg ccaacctcgg catcgccctg cagtatatca atatgcggag ttagag
//
LOCUS       DATGFG010001044         4864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1099585, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001044 DATGFG010000000
VERSION     DATGFG010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099585"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VKQ52_19855"
     CDS             <1..182
                     /locus_tag="VKQ52_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89523.1"
                     /translation="ATVTATTASSKKITGTFSFSARVITIDTTSSGRIDYKVDTVAVT
                     SGKFQNIPYSYIHRR"
     gene            complement(307..2424)
                     /locus_tag="VKQ52_19860"
     CDS             complement(307..2424)
                     /locus_tag="VKQ52_19860"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS2/Smr-associated SH3 domain-containing
                     protein"
                     /protein_id="HLZ89524.1"
                     /translation="MKIFPESALLQLEFDKVKTLLAEHCNTDYARSKAEDLRIHTRRE
                     FIELELRQSHEFKLLLQNGQYFPNDHTLNLARELKLLGIPGASLSGEQFLQIRRLAES
                     TDSIFRWFDAERRIAYPALARVIGNTHYEKAIVKAIDEVLDESGTVRDNASPELADIR
                     LSLYRKRNELRRVFDRIVSKLGKAGYVADIEESFLNGRRVVAIFAEHKRQVKGILHGE
                     SDTRRTSFVEPEETIELNNEVFSLEHAESKEVNRILRELTARLSGYAPLLQVWHDVLG
                     EFDFIRAKARLAGDMNGNFPLLLDKSHVGLRQAYHPLLLLYNRKNNKPTIPTNISLNE
                     KDRLLVISGPNAGGKTVTLKTVGLLQLMLQSGLLVPVHPDSEMGIFKQLMIHIGDTQS
                     IEFELSTYSSHLKNMKYFMENANGRTLFFIDELGSGSDPNLGGAFAEVILEELVRKHA
                     FGIVTTHYLNLKVMAGKTPGIINGAMAFDETNLLPLYKLTVGKPGSSYTFSIAERIGL
                     EPRLIQRARALVDEDHFRLDKLLNATEQDLRLLEKKEKDLHRLLKENEKLKKEMEALI
                     HRERHEQQVEILKHQNKITEERIAYLKEMERKLRQIIFDWRKTEDKNEVIKQMQALLF
                     KQKEKQVNEKVRKKFDAKYTEVGGNVELGNKVMMKKNHQVGTVKEIRGKKAIVQVGLI
                     PITVDLTDLTVVQDKPQEAIDGE"
     gene            complement(2517..3098)
                     /locus_tag="VKQ52_19865"
     CDS             complement(2517..3098)
                     /locus_tag="VKQ52_19865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HLZ89525.1"
                     /translation="MEKNSQALTTALLIMDIQPFTMGILGEGAPAFSTTLNKAIAAAR
                     NNDILVLFVVVGFRKGFPEITDRTSNKNLLVLRGTGMPGFENPAPDPSIDRAPDDIVI
                     AKRRVSAFTGSDLEVILRGHRIDHLVLTGLSTSGVVLSTLREAADKDYRLSVLSDACG
                     DRDPEVHNVLLNKVFPRQAEVLTTDQWIGSLPR"
     gene            complement(3074..4390)
                     /locus_tag="VKQ52_19870"
     CDS             complement(3074..4390)
                     /locus_tag="VKQ52_19870"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ89526.1"
                     /translation="MNKLTVFRAMQSRNYRLYFYGQTVSLIGTWMQRTAVSWVVYTLT
                     HSAFMLGLSLFCTQFPSFLLSTAGGVVSDRYHRFRVLLFTQIASGVQSLLLAILIFTG
                     HYNVWTILSLGIVLGAINAFDVPARQAMVYDMVDDKENLPNALALNSSMVNLARLVGP
                     AIAGFILHKLGDGPCFALNAASFIAVIISLLRMKLPPYVRKEHKKNALGELKEGWLYL
                     RNTPSIGRVILMLAAMSLLVIPYATLLPIYAKVIFHGNAATFGVIDSFIGLGAISGAL
                     YLATRTKNTEASRKKILRTNTIIFGVGLICFSYTTNFPLAMVFAVVAGFGMMAQTTIT
                     NTIIQTTVAPEMRGRVISYFAMSYFGMMPLGSLLVGAVSQYAGAPNTILAEGLVALVV
                     VAVYWKYLTTASTTRAATTPPTADANTSATVKQINTDTSWKKTARL"
     gene            complement(4383..>4864)
                     /locus_tag="VKQ52_19875"
     CDS             complement(4383..>4864)
                     /locus_tag="VKQ52_19875"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLZ89527.1"
                     /translation="SPKTALTPIHPAPQSTPDTRDAELASTFRTTIHRLVKLLRRQTR
                     NDAQLSLTERSTLGLLYPDSQLAPSDIARTEKVTTQSMSQVINHLAELDYIHRTPSVD
                     DKRKVLLSLTPAGRAYIEQIRHDKQEWLAHAIHERLTPAEKDTLAAAMNILTKLIDE"
BASE COUNT          840 a         1062 c         1818 g         1144 t
ORIGIN      
        1 aggcgacggt gacggctacc acggcttcca gcaagaaaat taccggtact ttttccttta
       61 gcgccagggt catcacgatc gatacgacga gttccggcag gatcgactat aaggtggata
      121 cggtggctgt cacgagcggg aagttccaga acattccgta tagttatatt caccggaggt
      181 gaggtgagtg aagcggcgtg ccggagaggt gttgatgggc gatggtggcg cgtcgatgag
      241 agcgggtgag gtgttgggcc gggaacgggt gaggtgcctg tgaatgcgcg tgaggcgttg
      301 ggcgggttac tcgccgtcga tggcttcctg gggtttgtcc tggacgacgg tgaggtcggt
      361 gaggtcgacg gtgatgggga tgaggccgac ctggacgatc gcttttttgc cgcggatctc
      421 ctttacggtg ccgacctggt ggttcttttt catcatgacc ttgttgccga gttcgacgtt
      481 gccgcctacc tcggtgtatt tggcgtcgaa tttcttccgg accttttcgt tgacctgttt
      541 ttccttttgt ttgaagagga gggcctgcat ttgcttgatg acctcattct tgtcctcggt
      601 cttgcgccag tcgaagatga tctgccggag cttgcgttcc atctccttga ggtaggcgat
      661 gcgttcttcg gtgatcttgt tctggtgctt caggatctcg acttgttgct cgtggcgttc
      721 gcgatggatg agggcctcca tttccttttt cagcttttcg ttttccttca ggaggcggtg
      781 gaggtccttt tctttctttt ccagaaggcg aaggtcttgt tcggtggcgt tcagcagttt
      841 gtcgagccgg aaatggtctt cgtcgacgag ggcgcgggcg cgctggatga gccggggttc
      901 gaggccgatg cgttcggcga tggagaaagt atagctgctg ccgggtttac cgacggtgag
      961 cttgtagagc gggaggaggt ttgtttcgtc gaaggccatc gccccgttga taatgccggg
     1021 ggtcttgccc gccatgacct tgaggttcag atagtgggtg gtgacgatgc caaaggcgtg
     1081 tttgcggacg agttcttcga ggatcacctc ggcaaaggcg ccgccgaggt tggggtcgga
     1141 gccgcttccc agttcatcga tgaagaagag ggtcctgccg ttggcgttct ccatgaagta
     1201 tttcatgttc ttcaggtgag aactgtatgt gctgagttcg aattcgatgc tttgggtgtc
     1261 cccgatgtgg atcatgagct gtttgaagat gcccatttcg ctgtcggggt ggacgggcac
     1321 gaggaggccg ctctggagca tgagctgcag gaggcctacg gtctttagcg tgacggtctt
     1381 accaccggcg tttggcccgg agatgacgag gagccggtct ttttcgttga gcgaaatatt
     1441 cgtcgggatc gtcggcttat tgttcttccg gttgtacagc aacaggagcg gatgataggc
     1501 ctgtcgcagg ccgacatggg atttgtccag cagtagcggg aagttgccgt tcatgtcgcc
     1561 ggcgaggcgg gcctttgcgc ggatgaagtc gaattcgccg aggacatcgt gccacacctg
     1621 caggagcggg gcgtagccgg agagacgcgc ggtgagttcg cggaggatgc gattgacctc
     1681 cttgctttca gcgtgttcga gggagaagac ctcgttgttc agttcgatcg tttcttcggg
     1741 ttcgacaaag ctggtgcggc gggtgtcgct ttcgccatgg aggatgccct ttacctggcg
     1801 tttgtgttcg gcgaagatgg cgacgacgcg gcggccgttg aggaaggact cttcgatgtc
     1861 ggcgacatag ccggccttgc cgagtttgct cacgatacgg tcgaagacgc ggcggagctc
     1921 gttgcgtttg cgatagaggc tgaggcggat gtcggcgagt tcgggggagg cgttgtcgcg
     1981 gacggtaccg ctttcatcca ggacctcgtc gatcgctttt acgatggctt tttcgtagtg
     2041 ggtgttcccg atgaccctgg cgagggcggg ataagctatc cggcgttcgg cgtcgaacca
     2101 gcggaagatg gagtcggtgc tttcggcgag ccggcggatc tggaggaatt gttcaccgga
     2161 gagagaggcg ccggggatgc cgagcagttt tagttcgcgg gcgaggttga gggtgtggtc
     2221 gtttgggaag tactggccgt tctggaggag gagtttgaat tcatgggatt ggcggagttc
     2281 cagctcgatg aattcgcggc gggtatggat acggaggtct tcggccttcg accgggcata
     2341 atctgtgttg caatgttcag ccaggagtgt cttcactttg tcgaactcca gttgcagtaa
     2401 ggctgattcg ggaaaaatct tcattcaatc ttttgggtgg acccgggccg gggcccgggt
     2461 cgattttgga gagcaaaggt acaaatttgt ctgctttgtc aatggcattt tgcggttcag
     2521 cgagggaggc tgccgatcca ctggtcggtg gtgaggactt cggcctggcg ggggaagacc
     2581 ttgttgagca ggacgttatg gacttcgggg tcgcgatcgc cgcaggcgtc ggatagtacg
     2641 gataaccggt agtccttgtc agcggcctcg cggagggtgg agaggacgac gccgctggtg
     2701 gagagcccgg tgaggacgag gtggtcgatg cggtggccgc gcaggatgac ttcgaggtcg
     2761 ctgccggtga aggcgctgac gcggcgtttg gcgatgacga tatcgtcggg ggcccggtcg
     2821 atggaggggt cgggcgcggg gttctcgaaa ccgggcattc cggtcccccg gaggacgagg
     2881 aggtttttgt tgctggtacg gtcggtgatc tccgggaagc ctttgcggaa gccgacaacg
     2941 acgaagagga cgaggatgtc gttgttgcgg gcggcggcga tggctttgtt cagggtggtg
     3001 gagaaagcgg gggcgccttc accgaggatg cccatcgtga aaggctggat gtccatgatc
     3061 aatagtgctg tggtcagagc ctggctgttt ttttccatga tgtatcggtg ttgatttgtt
     3121 tgacagttgc ggaggtgttt gcgtcggcgg ttggaggggt agttgcggcc cgggtggtgg
     3181 aggccgtggt gaggtatttc cagtagacgg cgacgacgac gagggctacg aggccttcgg
     3241 cgaggatggt gttcggtgcg ccggcatatt gggataccgc ccctacgagc aggctgccga
     3301 ggggcatcat gccgaagtag gacatggcga agtagctgat gacgcgaccg cgcatttcgg
     3361 gggcgacggt ggtttgaatg atcgtattgg tgatcgtcgt ctgggccatc atgccgaagc
     3421 cggcgacgac ggcgaagacc atggcgaggg gaaagtttgt cgtataggag aagcagataa
     3481 gccccacgcc gaagatgata gtgttggtac gcaggatctt tttgcggctt gcttccgtgt
     3541 tctttgtgcg ggtggcgagg tagagggccc cgctgatggc gccgagaccg atgaagctgt
     3601 cgatcacccc gaaggtggcg gcattcccgt ggaagatgac ctttgcgtag atgggcagga
     3661 gggtcgcata gggaatgacg aggaggctca tcgcggcgag catgaggatg acgcggccga
     3721 tggagggggt gttgcggaga tagagccagc cctctttcaa ttcaccaagg gcgttctttt
     3781 tatgttcttt acggacatag ggcggcagtt tcatacgcag cagggagatg atgacggcga
     3841 taaagctggc ggcgttgagt gcgaagcagg gcccgtcgcc gagtttgtgc aggatgaaac
     3901 cggcgatggc ggggccgacg aggcgggcga ggttgaccat cgaggagttg agggcgaggg
     3961 cgttcggcag gttctccttg tcatcgacca tatcgtagac catggcctgg cgggcgggga
     4021 cgtcgaaggc gttgatggcg ccgaggacga tgcccaggct gaggatcgtc cagacgttgt
     4081 agtgcccggt gaagatgagg atggcgagga ggagggactg gacgccggag gcgatctggg
     4141 tgaagaggag gacgcggaac cgatgatacc ggtcggatac cactccaccg gcggtggaca
     4201 ggaggaacga cggaaattgc gtacagaata aggagagccc cagcatgaag gcggagtggg
     4261 tgagtgtgta gaccacccag ctcacggcgg tgcgttgcat ccaggtgccg atgagcgaga
     4321 cggtctggcc atagaagtaa agccgatagt tacggctttg catggcgcgg aaaacggtta
     4381 atttattcat cgataagttt tgtcaggata ttcatggcgg cggcgagggt gtccttttcg
     4441 gccggggtta acctttcgtg gatggcgtgg gcgagccatt cctgtttgtc gtgccggatc
     4501 tgttcgatgt aggctctgcc ggccggggtg agggagagca ggactttgcg tttatcgtcg
     4561 acggaggggg tccggtggat atagtcgagc tcggcgaggt ggttgatgac ttgtgacatg
     4621 gattgggtgg tgactttctc cgttcgggcg atgtctgagg gcgccagttg gctatcggga
     4681 tagaggaggc cgagggtgga gcgttcggtg agggagagtt gggcgtcgtt gcgggtttgg
     4741 cggcggagga gtttgacgag ccggtggatg gtggtgcgga aggtggaggc tagttcggcg
     4801 tcccgggtgt cgggggtgct ttggggcgcg gggtgaatgg gggtgagcgc agttttgggg
     4861 gacg
//
LOCUS       DATGFG010001045         5000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1351432, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001045 DATGFG010000000
VERSION     DATGFG010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351432"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VKQ52_19880"
     CDS             <1..499
                     /locus_tag="VKQ52_19880"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HLZ89528.1"
                     /translation="WLAALLGIGYYFIKYYSWKVNIWVVTNFRVIDETGLLSHYAKES
                     PLDKINNISYDQTLLGRLFNYGHVEIQTAAEVGATDYYNVNHPKRLKDTITAAQADYK
                     NWQFSNQATQMAAALDSRNGNSVGPRAEAPQVAVELEKLFELKMKGAITEDEYLRAKD
                     RLLGR"
     gene            complement(496..1527)
                     /locus_tag="VKQ52_19885"
     CDS             complement(496..1527)
                     /locus_tag="VKQ52_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89529.1"
                     /translation="MRINQLTCNNTLVFLLTVCCLTGLSLPAGAQHLSAPQHLSGEAL
                     QEFAKREDSLQKFSYNIVNAEQATDRFRADSQFVRSLVRTLKMPNSFYYPFDSLQTVS
                     HLYAPDSSFRIFTWQFKKDEYIYLQEGAIQVNQPDGSLKLFPLFDASMFTERPLDSVR
                     TRRNWIGAIYYRIIEKTWQGRNYYTLLGFDDFSESSNKKWMEVLTFSETGEPRFGGPY
                     FSFVQDSIHKPIQYRFNIEYKKEAGTRFNYDSALDMVVYDHLVPEGDQPQKKDTYIPD
                     GDFEAFQWKDGRWIHVEKQLFSYKLKDGQFPMEAKMLDDQGNSNEAQLQEQSEKNLKK
                     KPVPVKKPG"
     gene            complement(1595..2152)
                     /locus_tag="VKQ52_19890"
     CDS             complement(1595..2152)
                     /locus_tag="VKQ52_19890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HLZ89530.1"
                     /translation="MIPFQQIDASWTLFLDRDGVINIEKENSYILHYGEFVFYEGVTE
                     ALRRCAAVFGHIVVVTNQRGVGKGMMTAADLQDIHEKMIAEIVLAGGRISRIYFADSL
                     DNAHPLRKPQPGMAHAAQRDLPGIDLSRSIMIGNNISDMEFGRNAGMYTVFLGTTNPE
                     QALPHPAIDMAFNSLADFAKHLRLA"
     gene            complement(2162..3307)
                     /locus_tag="VKQ52_19895"
     CDS             complement(2162..3307)
                     /locus_tag="VKQ52_19895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ89531.1"
                     /translation="MKGSVVIIGPAYPLRGGLATFDQRLAREFLAGGYDCLIYSFSLQ
                     YPGFLFPGTTQYSTDPPPDGLTILSRINSINPFNWWSVGEELRRRQPDLIVVRYWLPF
                     MGPALGTILRRVRKNGHTRVIAITDNVIPHEKRPGDEPFTRYFLKACDGFITMSEKVM
                     NDLRRFQPLKPAQQVAHPLYDNFGSSIATAAARQLLREKGVAIGDDDRVLLFFGFIRK
                     YKGLDIVLEAMADKRIGDLGIKLLVAGEFYEDAAPYQELITRLGIGHRLLLRTDFIPD
                     SEVRDYLCAADAVIQPYRNATQSGVTPLAYHFEKPMIVSNVGGLPALVPHEKVGLVAD
                     PEPTAMADAILRFFVLGGSYFLPALRSEKEKYSWSRLVETILSLSKS"
     gene            complement(3315..4262)
                     /locus_tag="VKQ52_19900"
     CDS             complement(3315..4262)
                     /locus_tag="VKQ52_19900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ89532.1"
                     /translation="MELSIVIPVYNEDESLPELCSWIERVMRENGFSYEVILVDDGST
                     DRSWEVIQHLRNSDPNIKGIKFQRNYGKSAALNEGFKAARGEVVITMDADMQDSPDEI
                     PELRRMILEGRYDMISGWKKVRHDPISKTLPSKFFNWTTAKVSKIPLHDFNCGLKAYR
                     NKVVKSIEVYGEMHRYIPVIAKWSGFKKIGEKVVQHRPRKYGHTKFGGLGRGLKGLLD
                     LASITFVGKYGKRPMHFFGPWGVICGLIGFGETISLLIQKLNNMSTFTLTNKPSFYIA
                     LTCMVLGTQLFLTGFVAELISRNSPGRNSYLIEQKLGLE"
     gene            complement(4272..4814)
                     /locus_tag="VKQ52_19905"
     CDS             complement(4272..4814)
                     /locus_tag="VKQ52_19905"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HLZ89533.1"
                     /translation="MAATKKIPVPVLYGAIAALGMILVTLATYYGGVDMFIGNVAYLM
                     YLFPIALAVVGALVQKKVNGGILGFQDALKACFGVIVLALAVQTLFTWILVRFIDPRF
                     GQALPAAVLAKTEATFRRFGVPEDEISKTLAEEKGKDPFSLGRMLTGLARNYIVGFPV
                     AVLLAAIIGQRLVAGRGPKA"
     gene            complement(4817..>5000)
                     /locus_tag="VKQ52_19910"
     CDS             complement(4817..>5000)
                     /locus_tag="VKQ52_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89534.1"
                     /translation="QPSIQEGQVAALSFFDPTGKTTLRTSVSKSKNTPFLGRTLQGQV
                     LGILNGEQLHLNQPEQ"
BASE COUNT         1082 a         1384 c         1427 g         1107 t
ORIGIN      
        1 ctggctggct gcgctgctgg gcatcgggta ttatttcatc aaatattaca gctggaaggt
       61 gaatatctgg gtggtgacca atttccgggt catcgacgag accggtctgc tcagtcacta
      121 tgccaaggaa agcccgctgg ataagatcaa taatatctcc tatgaccaga ccctgcttgg
      181 ccggctgttt aactacggcc atgtggagat ccagacggcg gcggaagtag gggccaccga
      241 ctattacaac gtcaaccatc ccaaacgcct gaaagatacc atcacggccg cccaggccga
      301 ttataagaac tggcagttca gcaaccaggc cacgcagatg gcggcggccc tggactcccg
      361 caacggcaat agcgtggggc caagagcgga agccccacag gtggcggtcg aactggaaaa
      421 gttatttgaa ctgaaaatga agggcgccat caccgaagac gaatacctcc gcgccaagga
      481 tcggctcctg ggccgttaac ccggcttttt caccgggacc ggttttttct tgagattctt
      541 ctccgactgt tcctgcaact gcgcctcatt ggaatttccc tgatcatcga gcatcttcgc
      601 ttccatcggg aactgtccat ccttcagctt gtaggagaag agctgtttct ccacatggat
      661 ccacctgcca tccttccatt ggaaggcctc gaagtcaccg tcggggatat acgtatcctt
      721 tttctgcggc tgatctcctt ccgggacaag atgatcatag accaccatat ccagtgcaga
      781 gtcgtagttg aaccgggtcc cggcctcctt cttgtattcg atattgaagc ggtattggat
      841 gggtttgtgg atggagtcct gtacaaaaga gaaatatggt ccgccgaacc ggggttcgcc
      901 ggtttcgcta aaggtgagca cctccatcca tttcttgttg ctcgattcgc tgaagtcgtc
      961 aaaacccagc agtgtgtaat agttgcggcc ctgccaggtc ttctcgatga tgcggtagta
     1021 gatcgcgccg atccagttcc ttcgcgtccg caccgaatcc agcggtcttt cggtgaacat
     1081 ggaggcatcg aacagcggaa ataatttcag cgagccgtcg ggttggttca cctggatggc
     1141 cccttcctgc agatagatgt actcgtcctt cttgaattgc caggtaaaaa tgcggaagct
     1201 gctgtcggga gcgtagagat gggacaccgt ctgcagggaa tcgaaggggt aataaaaaga
     1261 gttgggcatt ttcagcgtcc ggaccagcga ccgtacgaac tggctgtccg ccctgaaccg
     1321 gtctgtcgcc tgctccgcgt tgacgatatt gtaggagaac ttttgaaggg aatcttcccg
     1381 cttcgcgaat tcctgcagcg cctccccgga caaatgctgc ggcgcggaca aatgctgcgc
     1441 cccggcgggg agggaaagac ccgtgagaca gcacaccgtc aggaggaaaa caagggtgtt
     1501 gttacaggta agctggttga ttcgcatggt gatgtaaagt tacaagtgaa atgacggatc
     1561 ccgacaggcc aaacggtaaa tccgggtgaa aattttatgc caatcgcaaa tgttttgcaa
     1621 aatcggccag cgaattgaat gccatatcga tagcagggtg gggaagggct tgttcggggt
     1681 tggtggtgcc caggaaaacg gtatacattc ccgcattccg cccaaattcc atatcgctga
     1741 tattattccc tatcatgatg cttcgcgaga ggtcgatccc ggggaggtcg cgttgcgccg
     1801 catgggccat cccgggctgg ggcttgcgca ggggatgggc attatccagc gagtcggcga
     1861 aatagatacg ggagatccgt ccccccgcca gtacgatctc cgcgatcatc ttctcatgga
     1921 tatcctgcag atcggccgcc gtcatcatcc ctttcccgac gccccgctgg ttggtcacga
     1981 ccacgatgtg cccgaagacg gcggcacagc gccgcagggc ttccgtgaca ccctcgtaga
     2041 atacgaattc tccatagtgc aggatataac tgttctcctt ttctatgttg atgaccccgt
     2101 cacgatcgag gaagagggtc caggaagcgt ctatttgctg aaatgggatc atgggctaag
     2161 actaagattt gctaaggcta agaatggttt ccaccagccg gctccaggaa tatttttcct
     2221 tctccgaccg gagggcggga agaaaataac taccccccaa aacaaaaaaa cgcaggatcg
     2281 catcggccat tgccgttggt tcggggtccg ccaccagccc cactttctca tgcggcacaa
     2341 gcgccggcag accgccgaca ttcgatacga tcatcggttt ctcgaaatga taggcgaggg
     2401 gcgtgacgcc gctttgcgtg gcgttacgat agggttgtat cacggcatcg gcggcacaga
     2461 gatagtcccg tacctcgctg tcggggatga aatcggtgcg cagcagcagc cggtggccga
     2521 tccccagccg ggtgatcagt tcctggtagg gcgcggcgtc ctcgtagaat tccccggcta
     2581 ccagcagctt gatgcccaga tcgccgatcc gcttgtcggc catggcctcc agtacgatat
     2641 cgagcccttt gtatttgcgg ataaaaccaa agaagagcag cacccggtcg tcgtcgccga
     2701 tggccactcc cttttcccgc agcaactgcc gggcggcggc ggttgcgatg gaggagccaa
     2761 aattatcgta cagcgggtgc gccacctgct gggccggctt caggggttga aaacgccgca
     2821 gatcgttcat cactttttcg ctcatcgtga taaaaccatc gcaggccttc aggaaatacc
     2881 gggtaaaggg ttcgtcgccc ggccgtttct catgcgggat gacattgtct gtgatggcga
     2941 tgacccgggt atgcccgttc ttcctcaccc gccgcaggat ggtcccgagg gcgggtccca
     3001 taaagggcag ccagtaccgg acaacgataa gatccggttg ccgccggcgc aattcttctc
     3061 ccaccgacca ccagttgaag ggattgatcg aattgatccg ggacaggatg gtgagtccgt
     3121 ccggtggggg atcggtgctg tattgcgtgg tgccgggaaa aaggaacccg ggatattgca
     3181 gggaaaagga atagatgagg cagtcgtatc ccccggccag gaactcacgg gcaagccgct
     3241 ggtcgaaggt tgccaggccc ccccgaagcg ggtaagccgg accgataatg acgacagaac
     3301 ctttcatatc tattctattc gagtcccagt ttttgttcga tgagatagga attgcggccc
     3361 ggggaattcc gggagatgag ttcggcgaca aagccggtga gaaaaagctg ggtccccagc
     3421 accatgcagg tgagcgcgat gtagaaagag ggcttgttcg tcagcgtgaa cgtggacata
     3481 ttgttcagct tctggatcag cagcgagatc gtctcaccaa aaccgatcag cccgcagatg
     3541 accccccagg ggccaaagaa gtgcatcggc cgtttgccat acttgccgac aaaggtgatc
     3601 gacgccagat cgagcaggcc cttcagcccc cggcccaggc cgccaaactt ggtgtggcca
     3661 tattttcttg gccggtgctg gactactttc tccccgatct ttttgaagcc cgaccatttc
     3721 gcgatcacgg ggatataccg gtgcatctct ccatagacct cgatgctttt taccactttg
     3781 ttgcgatagg cctttaaccc gcagttgaag tcgtgcaagg ggatcttgct gacctttgcg
     3841 gtcgtccagt tgaagaattt ggaaggaagg gtcttggaga tgggatcatg acgaaccttc
     3901 ttccagccgc tgatcatatc gtaacggcct tcgaggatca tccggcgtaa ttcggggatc
     3961 tcgtcgggac tgtcctgcat gtcggcgtcc atcgtgatca cgacctctcc gcgggcggcc
     4021 ttgaaccctt cattgagggc ggcggacttg ccgtaattgc gttggaattt gatccccttg
     4081 atattgggat cggagttgcg caggtgctgg atcacctccc agctgcgatc ggtgctgcca
     4141 tcgtccacca ggatcacttc ataggagaat ccattctccc gcatgaccct ttcgatccag
     4201 ctgcacagct cgggcaggga ttcatcctcg ttgtagaccg gtatgacgat tgacaattcc
     4261 attccagttg tttatgcttt tgggccccgg cccgctacca ggcgctgacc gatgatggca
     4321 gccagcagca ccgcgacggg gaatccaaca atataatttc gtgccagacc ggtgagcatc
     4381 cgccccaggc taaaggggtc cttccccttc tcctcggcga gggtcttgct gatctcgtct
     4441 tccgggacgc cgaagcgccg gaaggtggcc tctgtcttgg ccagcaccgc ggccggcagc
     4501 gcctggccaa agcggggatc gataaaccgc accaggatcc aggtgaaaag ggtctgtacg
     4561 gcgagggcca ggacgatcac gccaaaacat gcctttagtg cgtcctggaa acccagtatc
     4621 cccccgttca ctttcttttg gaccagggcc cctacgacag ccagggcaat agggaaaaga
     4681 tacatcaggt aagcgacatt cccgatgaac atgtccaccc cgccataata ggtggccagc
     4741 gtcaccagga tcatacccag cgcggcgatg gctccataca acacgggtac cgggatcttt
     4801 tttgttgcgg ccatcgttat tgttccggtt gatttaggtg tagttgctct ccgttcagta
     4861 tgcccagcac ctgcccctgc agggtcctgc cgagaaaagg agtattcttc gacttggata
     4921 cgcttgtcct cagggtcgtt tttccggtgg ggtcgaagaa gctgagtgcc gccacctggc
     4981 cttcctggat ggaaggctgc
//
LOCUS       DATGFG010001046         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_932854, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001046 DATGFG010000000
VERSION     DATGFG010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932854"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VKQ52_19915"
     CDS             <1..848
                     /locus_tag="VKQ52_19915"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HLZ89535.1"
                     /translation="IDAAHITFFLLPFFLLAFLLYRLFLRVRTEKHNRIYEARHADFD
                     GLLSRYNPYYRSLSRAGRDRFLHRVLLFMEAKKFEYIDVEPDESMPLLVSAAAVQLTF
                     GLEHYRLDYFRAIHILKDKYRFGLYTTPFEGHVSEDGIYLSWNHFIREFTDYSDGQNV
                     GLHEMAHALTYVNFTVHDGRDNTFHDRFTEFSAVARPIFERMQAGESTLLDSYAATNY
                     QEFWAVCIETFFERPTPFRRQLPELYFSLCSLLNQDPLTPNKVLNINSIDSDEINGPL
                     APTGS"
     gene            1057..1929
                     /locus_tag="VKQ52_19920"
     CDS             1057..1929
                     /locus_tag="VKQ52_19920"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HLZ89536.1"
                     /translation="MEIIVIFSLVLGILLLNRPVTLLFQRLYTKHQFKRFVAKETFYH
                     SVVSRHFRYYNRLGLEDQRRFLFRTWLFQHSKNFHYIEVQQSAEMPILVSAAAVQLTF
                     GLDKFKLNYFDDIFILRDDYHYGFYSRPFMGHVDQTGIYLSWDNFMRGITGQTANCNV
                     GLHEMAHALAYVNFITQTEEDKHFKKEFPNFSKVARPIFTSMQGEGGTRNLLGDYAST
                     NYHEFWAVAVEVFFESPVQFRHELPDLYAAMSRVLNQDPLACLSGSTTIIMRPEPLTQ
                     KTLVGAEVAVAHAH"
     gene            complement(1933..3054)
                     /locus_tag="VKQ52_19925"
     CDS             complement(1933..3054)
                     /locus_tag="VKQ52_19925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLZ89537.1"
                     /translation="MGQRVEFLIIGQGICGSFLQLGLERAGLSHIVIDEPQPFSASRV
                     AAGLINPVTGRRLVTTWMIDELLSFAREAYQGLTERLGASFFSPVMVADLFPTAQMRL
                     AFLKRLEEDGQYLRLPEDEHDLLDRFHYELGYGLIHSCYLVDLPGLLSAVRRRMAGQG
                     ILREERFHQQELVVEDTHVLYRDIEARRILFCDGAAGFDNPYFSRLPFAPNKGEALIV
                     EIPGLEDAAQWKNDAHPSGERLVVGERMVFKKGISLVPWREGLYWAGSSYEWSFEHAQ
                     PTELFRLRTEAALREWLKPPFRVVEHVAAVRPATLERRPFVGFHPVYPAVGILNGMGT
                     KGCSLAPYFAHELVQQVLTGSPIRADADVKRFTKVLSRK"
     gene            3104..3610
                     /locus_tag="VKQ52_19930"
     CDS             3104..3610
                     /locus_tag="VKQ52_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89538.1"
                     /translation="MEGNRLTQRLAFFGFRKIFDSINQHGIQKGVDRMGIDGFIEHCA
                     RLAASTGAITGMGGAITMIVGIPADLFNNFTQQFRVTLGVIYARRGSYTVSFEELMSV
                     VGVSLGLEAGLILTREILENVAEKMLIRMGAKAGGRLIPFVGAVVGGTTNYLFIKSVG
                     ASVKKLPL"
     gene            complement(3669..>4188)
                     /locus_tag="VKQ52_19935"
     CDS             complement(3669..>4188)
                     /locus_tag="VKQ52_19935"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89539.1"
                     /translation="KGNGSPAWGAVYWQYFDMLDRITPPGQGKAPLRIEKRLFLSRNT
                     DRGPVLEPIADNGALKPGSKVVVRIVIVADRDLEYVHMKDMRGACFEPVNVISQYKWQ
                     DGLGYYESTKDASTDFFFGWLPKGTHVFEYPMFTGQAGNFSNGVTSIECMYAPEFASH
                     TEGIRVNVEAAP"
BASE COUNT          944 a         1324 c          993 g          927 t
ORIGIN      
        1 gcatcgatgc tgcccatata accttcttcc tcctgccatt cttcctgctg gccttcctcc
       61 tctaccggct cttcctccgc gtccgcacgg aaaaacataa ccgcatttac gaagccaggc
      121 acgcagactt cgacggcctg ctctcgcgct ataaccctta ctaccgcagc ctgagcaggg
      181 ccggacgcga ccggttcctg caccgtgtgc tgctttttat ggaagcaaaa aaatttgaat
      241 atatcgacgt ggagcccgat gagagcatgc ccctgctcgt cagcgcggct gccgtgcagc
      301 tcacctttgg cctcgaacat taccggctcg attatttcag ggccatccat atcctgaagg
      361 acaaataccg cttcgggctc tataccactc ctttcgaagg acatgtgagc gaagacggca
      421 tctatctctc ctggaaccat tttatcaggg aattcaccga ttattccgat ggccagaatg
      481 taggtcttca cgagatggct catgcgctca cctatgtcaa tttcacggtc catgacggcc
      541 gcgataatac cttccacgac cggttcacgg agttttccgc tgtcgcccgg cccatcttcg
      601 aacggatgca ggccggcgag agcaccctcc tggactccta tgcggccacc aactaccagg
      661 aattctgggc cgtctgcatc gaaaccttct tcgaacgccc cacccccttc aggcggcagc
      721 tgcccgaact ctacttttcg ctttgctccc tgcttaacca ggatccgtta actcccaata
      781 aagtgctgaa tatcaattca atagactctg atgaaataaa cggcccgctg gcgccgaccg
      841 gttcctgatc ctaaaatgta aaggaaaaat ttccccaggg tttttctttt tcaggaattc
      901 tttttatttt gtctcccatc cgtacgattc attcctcttg attaaaccca cctcaggttt
      961 tccactctaa tctggtagcc tttgttccgt atccatagcc aatgtaagac ctggtttatt
     1021 ttattatttc tttagactac aaagaaaatg aaccttatgg aaatcattgt tatcttttca
     1081 ttggtgctgg ggattcttct tctcaatcgt ccggtcacat tgctttttca acgactctac
     1141 acaaaacacc agttcaaacg gttcgttgca aaggagactt tctatcattc ggtggtttcg
     1201 cgtcatttcc ggtattataa ccgcctggga ctggaggatc agcggagatt cctctttcgc
     1261 acctggctct tccagcactc caagaacttc cattatatag aagtacaaca gtcggccgaa
     1321 atgccgatcc tggtcagcgc cgccgccgta caactgacct ttggcctgga taagttcaag
     1381 ctcaactact tcgacgatat cttcatcctc cgggatgact atcactacgg attctattcc
     1441 cgccccttta tgggtcatgt ggaccagacg gggatctatc tctcctggga taattttatg
     1501 cgcggcatca ccggccagac ggccaactgc aacgtcgggc tccatgaaat ggcccacgcc
     1561 ctggcctatg tcaattttat cacccagacc gaggaagaca aacacttcaa aaaggaattc
     1621 cccaacttct ccaaggttgc ccgccccatc ttcacctcca tgcaggggga gggggggaca
     1681 aggaacctgc tcggcgacta cgcctctacc aactaccacg agttctgggc ggtggccgtc
     1741 gaagtgttct tcgagagccc cgtgcagttc cggcatgaac tccccgatct ctatgcggcc
     1801 atgtcccgcg tcctcaacca ggaccccctg gcttgtctca gcggcagcac gaccatcatc
     1861 atgcggcccg aacccctcac gcaaaaaacg cttgtggggg ccgaagtcgc cgttgctcac
     1921 gcccattaga tctcatttcc tgcttaaaac ctttgtaaat cttttcacat cggcgtctgc
     1981 ccgtatggga ctgccggtca aaacctgttg taccaactca tgcgcaaaat aaggagccag
     2041 cgaacatccc ttcgtaccca tgccattcag tatgcctaca gccggataaa caggatgaaa
     2101 tcctacaaac ggtcttcgct ccagcgtcgc cggtcgcacc gcagccacat gctccacaac
     2161 gcggaagggc ggcttcagcc attcccgcaa cgccgcttcc gtccgcagcc ggaaaagttc
     2221 cgtcggctgc gcatgttcaa acgaccattc ataagaagaa cctgcccagt atagtccctc
     2281 ccgccagggt acaaggctga tccccttctt aaagaccatc cgctccccaa cgaccagccg
     2341 ctccccggat gggtgggcgt cattcttcca ttgggcagca tcttccagtc ccgggatctc
     2401 cacgatcagg gcctctcctt tattcggcgc aaagggcagc cgcgaaaaat aaggattgtc
     2461 aaaaccggcg gccccgtcgc aaaagaggat gcgccgggct tcgatgtccc ggtacagcac
     2521 gtgcgtgtcc tccaccacca gctcctgctg gtggaaccgc tcctcccgga gaatcccctg
     2581 gccagccatc cgccggcgga cggccgaaag caggcccgga agatcgacga gataacagga
     2641 atggatcaat ccgtacccca gctcgtaatg gaaccggtca aggagatcgt gttcgtcctc
     2701 cggcagccgg agatactgcc cgtcctcctc caaccgtttc agaaaggcca atcgcatctg
     2761 cgccgtgggg aaaagatcgg ccaccatcac cggcgaaaaa aaagaagcgc ccaggcgttc
     2821 ggtcagtccc tggtaggcct cgcgcgcaaa agacagcagc tcatcgatca tccaggtggt
     2881 gacgagacgc cgccccgtca ccgggttgat aagccccgcc gcaacccgcg aggcactaaa
     2941 cggctgcggc tcatcgatca cgatatggga caaaccggcc ctttccaacc cgagctgaag
     3001 aaaacttcca caaataccct ggccgatgat caagaattct acccgttgtc ccatccttgg
     3061 tttacaattt gcaggaaaga taattattaa atcggagggg aatatggaag ggaaccgcct
     3121 cacgcaacgc ctcgctttct tcggcttcag gaagatcttc gacagcatca accagcacgg
     3181 catccagaag ggggtcgacc ggatggggat cgacggcttt atcgaacatt gcgcgcggct
     3241 ggccgcctcc acgggcgcca tcaccgggat gggcggcgcc ataacgatga tcgtgggcat
     3301 ccccgccgat ctgttcaaca attttacgca gcagttccga gttacgctgg gggtcatcta
     3361 cgcccgccgc ggcagctata ccgttagctt tgaagagctg atgtcggttg tcggcgtttc
     3421 ccttgggctc gaagcgggcc tgatcctgac ccgcgagatc ctcgaaaatg tcgccgaaaa
     3481 gatgctgatc cgcatgggcg caaaagccgg cggccgcctc atccctttcg tcggcgccgt
     3541 ggtcggcgga acgacaaact atctctttat aaaaagcgtc ggcgcatccg tcaaaaagct
     3601 cccgctttaa ggcgccggag gggcttgtcc ggacatctaa gacaccagag gcagcttgcc
     3661 agcacatttt acggcgcggc ctccacgttc acgcggatcc cctcggtatg agaagcgaat
     3721 tcgggggcat acatacattc gatactcgta accccattgc tgaaatttcc cgcctggccc
     3781 gtaaacatcg ggtattcgaa aacatgggtt cccttcggca gccagccaaa aaagaaatcg
     3841 gtgctcgcat ccttcgtgct ctcataatag ccgagcccat cctgccactt atactggctg
     3901 atcacgttca ccggctcaaa acatgcgccc cgcatatcct tcatatgcac atattccagg
     3961 tcacggtcgg caacgatcac gatccgcacc accaccttac ttccgggctt cagcgccccg
     4021 ttatcggcga taggctccag caccggcccc cgatcggtat tccgcgaaag gaataatctc
     4081 ttctcgatcc gcagtggcgc cttgccctgc cccggcggcg tgatccggtc cagcatgtcg
     4141 aaatactgcc agtagaccgc accccacgca ggagaaccat ttcccttc
//
LOCUS       DATGFG010001047         4419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_289373, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001047 DATGFG010000000
VERSION     DATGFG010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289373"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..2226
                     /locus_tag="VKQ52_19940"
     CDS             235..2226
                     /locus_tag="VKQ52_19940"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HLZ89540.1"
                     /translation="MSYCLSFPITVAAFLCISLTLRAQEPVRVTLHLEEPGAAVSPGL
                     YGLMTEEINFSYEGGLYGELIRNRSFKDSAKVAAFWSLMPGTSGKATMSLDKQNPVSP
                     ALPVSLRLDVENAGDGVGIANEGYWGMAVRPNTTYKGSFFVRSSAGGGQSLRVALESR
                     DGKTVFASSEVAAPGADWKRAGFSLTTTAGIVPTADARFVIRAGATGSYWFSLVSLFP
                     PTYKDRRNGNRQDIMQLLADMKPAFLRLPGGNYVEGNTFSQRWDWKKTIGPIEQRPGH
                     LSPWHYRSTDGLGLLEFLEWCEDLDMEPLVAVFAGYVLNRDYLEAGPFLQPFVDDALD
                     EIEYITGDVNTKWGAQRAKDGHPQPFPLHYIEIGNEDGFDASGSYEGRYAQFHDAIKA
                     RYPQLHIISTVGGKDGLGGRFPAPAGRTEIVDEHYYRNAWQMEENAGQYDSYDRNGPK
                     VFVGEWATREGDPTTNFNAALGDAAWMTGMERNSDLVIMSCYAPLFVNVNPGGMQWKS
                     DLIGYNALTSFGSPAYHAQKMFSTHLGDRVIKMTMENIPSQLQKLGARDSAAGVQPKK
                     IDALFFSATHDTKKGLIYLKVVNTQAVAQAIAINLAGVGKVAKEGTAWTLAAAGPTET
                     NSITEPRKIVPVESVVKGLGKAFVRTFPPYSITVLQIKD"
     gene            2236..2889
                     /locus_tag="VKQ52_19945"
     CDS             2236..2889
                     /locus_tag="VKQ52_19945"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89541.1"
                     /translation="MLQTFSHHSFQGLCDGLAAEDADLAAVIKAHGYPPMWNRANSFE
                     TLVHIILEQQVSLASALAALNKLREFIGEVTPEKALVLTDEEWRACYFSRQKAEYVKY
                     LAAELLIGDLDLPGMETLPDEVVRERLTELKGIGNWTVDVYLIFVLQRADIFPMGDLA
                     AVNALKRLKGIPKEAHRDSLLPVIERWRPYRTIATMLLWHFYLSSPVRKQPEMQLNG"
     gene            2958..3728
                     /locus_tag="VKQ52_19950"
     CDS             2958..3728
                     /locus_tag="VKQ52_19950"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89542.1"
                     /translation="MKKIRLFFSLCFLLSGLVNTTTAQIHISINILPAEPFVERPPQP
                     TTNHVWMEGEWVAQNNGYVHQPGHWVVPPKDQIWVKGEWIHNPDGSNYWQPGYWKPIP
                     HMGVPMWIPEPNYLRPPRPSGRHLWVNGEWIWEGNHYMYQTGYWTVPEPNKVFIKGHW
                     AQWPNGEWYWVMGYWRRIPDNVFVSACPAEPGFNRPPAPSSDHVWIEGEWIWQNNGYV
                     WKPGYWFQRDPHRLWVRGYWHQRQDGGWYWVEGYWEWV"
     gene            3824..4330
                     /locus_tag="VKQ52_19955"
     CDS             3824..4330
                     /locus_tag="VKQ52_19955"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ89543.1"
                     /translation="MSNRKWFEREFDLSFGQDEYTAIYERLRTAPEKLRQVVAGLPEE
                     VLLQKPDGKWSVKEHAGHLSVLEPLWRIRFQDIREGKPVLTTADLNNTATSNAGFNDY
                     PVAALVEQFAKERSATLSLLNSIDVLDEEHTSLHPRLQQPMRIIDLAYFVAEHDEHHF
                     AAMREMSR"
BASE COUNT          965 a         1013 c         1428 g         1013 t
ORIGIN      
        1 ttatgttggt tcggtgctga aggatgaaaa ggggaatcag gtgacctatg tgcttcagca
       61 tctcgatacc ggcaaaacga tggacgaggg gatgttcctt atcccggacg ggtatgagaa
      121 gatgacgcat gcccagttca aggagagact gagggcaaaa ttgcgggggg gcaaataatc
      181 ctgttttgtt ggtaagatgt tatatttaca tttattccaa caaaatcgat aacgatgtcg
      241 tattgccttt ctttccccat taccgttgct gcatttcttt gtatttcgct gacgctgcgg
      301 gcgcaggagc ccgtccgtgt aaccttacac ctggaggagc ccggtgcggc cgtgagtccc
      361 ggtttatatg gattgatgac ggaggagatc aatttctcct acgaaggcgg cctgtatggc
      421 gagctgatcc ggaaccggag ctttaaagac tccgcgaagg tggccgcttt ctggtcgctg
      481 atgccgggta catcggggaa ggcaacgatg agccttgaca aacagaaccc cgtgagcccc
      541 gcgctacccg tttcgctccg gctggatgtg gagaatgccg gcgacggtgt cgggattgcc
      601 aatgaaggtt actgggggat ggcggtgcgg ccaaatacaa cctataaagg aagtttcttt
      661 gtacggtcgt cggccggggg cggccagtcc ctgcgggtgg cgttggagag cagggatggg
      721 aagacggtgt ttgcctcctc ggaggttgcg gcgccgggtg cggactggaa gcgtgcgggt
      781 tttagcctta caacgaccgc gggtattgtc cctacggcgg atgcgcgttt tgtgatcagg
      841 gccggggcga cgggcagcta ttggtttagc ctggtgtcat tgttcccgcc aacctacaag
      901 gatcgccgca acggcaaccg acaggatatc atgcagctgt tggcggatat gaagccggcc
      961 tttttgcggt tacccggtgg caactatgtg gagggaaaca ccttttcgca gcgctgggac
     1021 tggaaaaaaa cgatcgggcc gatcgagcaa cgaccgggtc atctgagccc ctggcattac
     1081 cggagtacgg atgggttggg gttgctggaa ttcctggagt ggtgtgaaga cctggatatg
     1141 gagccgttgg tggccgtttt tgcggggtat gtgctaaaca gggactatct cgaagcgggg
     1201 cccttcctcc aaccctttgt ggacgacgcc ctcgatgaga tagaatatat aacaggagat
     1261 gtgaatacga agtggggcgc gcagcgggcg aaggatgggc atccgcagcc ttttccgctg
     1321 cactatatcg agatcgggaa cgaggacggg ttcgatgcgt cgggcagtta cgaggggcgt
     1381 tatgcgcaat tccatgatgc gataaaggca aggtatccgc agttgcatat catttccacg
     1441 gtgggtggca aagatggact gggagggcgg ttccccgcgc cggcgggaag gacggagatc
     1501 gtggatgagc attattaccg gaacgcctgg cagatggagg agaacgcggg tcaatatgat
     1561 agctatgacc ggaacgggcc gaaggtattt gtcggggagt gggcgacgag ggaaggcgat
     1621 cccaccacga attttaatgc tgcgcttggg gatgctgcct ggatgacggg gatggagcgg
     1681 aattccgacc ttgtgatcat gtcctgttat gccccgctgt ttgtgaatgt caacccaggt
     1741 gggatgcaat ggaagagcga tcttatcggt tacaatgcgc ttacttcttt tggttcgccg
     1801 gcctatcatg cgcagaaaat gttcagcacg catctgggtg accgggtcat caaaatgacg
     1861 atggagaata tcccgtcgca gctgcagaag ctgggggcaa gggactcggc ggcgggtgtg
     1921 cagccaaaga agatcgatgc gctgttcttt tcggctactc acgatacaaa gaaagggctt
     1981 atctacctga aggtggtgaa tacccaggcg gtggcgcagg cgatagcgat caacctggcg
     2041 ggtgtgggga aggtggcgaa ggagggaacg gcctggacgc ttgcggccgc gggtccgacg
     2101 gaaaccaatt cgatcacgga gccgcggaag atcgtcccgg tggaatccgt tgtaaagggt
     2161 ttgggtaagg cctttgtccg gactttcccg ccttattcta tcacggtact gcaaataaag
     2221 gactaaggtt ttcccatgtt gcaaactttt tctcatcata gctttcaggg cctttgcgac
     2281 gggcttgccg cggaagatgc cgatctggcc gcggtgataa aggcccatgg ctatccgccg
     2341 atgtggaacc gggccaatag tttcgaaacg ctggtgcata tcatcctgga gcagcaggtt
     2401 tcgcttgctt cggcgttggc ggcgctgaac aagttgcggg agttcatcgg cgaggtcacg
     2461 ccggagaagg cgcttgtgct gacggatgag gagtggcggg cctgttattt cagccggcag
     2521 aaggcggagt atgtaaaata cctggccgcg gagctgctga tcggggatct cgatctgccg
     2581 gggatggaga cgctgcccga tgaggtggtc cgggagcggc tcaccgagtt gaaaggcatc
     2641 ggcaactgga ccgtcgatgt ctacctgatc tttgtgttac aacgggccga catctttccg
     2701 atgggtgatc ttgcggccgt gaacgccctg aaacgcctga agggtatccc caaggaggcg
     2761 catcgcgatt ccctgttgcc cgtgatcgag cgatggaggc cgtaccggac gatcgcgacg
     2821 atgttgttat ggcattttta tctttcgtca ccagtgagaa agcaacccga gatgcaattg
     2881 aatggttaat tgtcctgaaa aagcggcaac tggttcaaca tttgagtagg gttcatgact
     2941 taaaaatcta ctacgtcatg aaaaaaatcc ggttattttt ttcgttgtgt ttccttttgt
     3001 ccgggctggt gaacacaacg actgcacaga ttcacatttc catcaacatt cttcccgcag
     3061 agccttttgt cgagcgaccg ccgcagccga ccaccaatca tgtctggatg gaaggcgagt
     3121 gggttgccca gaacaacggt tacgtgcatc agcccggcca ttgggtggtg ccgccgaagg
     3181 accagatctg ggtaaaaggg gaatggatcc acaatcctga cggcagcaac tattggcagc
     3241 cgggatattg gaaacccatt ccgcatatgg gggtgccgat gtggataccc gagcccaatt
     3301 atctccgtcc cccccgccct tccggtcgtc acctgtgggt gaacggcgaa tggatctggg
     3361 agggcaatca ctatatgtac cagacgggtt actggacggt ccccgagccc aataaggtct
     3421 ttatcaaggg gcactgggcg cagtggccga acggcgagtg gtattgggtg atgggttact
     3481 ggcggcggat acccgataat gtttttgtca gcgcctgtcc tgccgagccc ggctttaacc
     3541 ggccgcctgc gccttcatcc gatcatgtat ggatcgaggg ggaatggatc tggcagaaca
     3601 atgggtatgt ctggaagccg ggttattggt tccagcgcga tccgcacagg ctttgggtac
     3661 ggggatactg gcatcagcgg caggatggag ggtggtattg ggtagagggg tattgggagt
     3721 gggtataatg tcagtatata ctatcagtat gagcggcccc cgctttgcgg gggccgcttc
     3781 gtttaaattg ctttatattt atatctatag aaaattataa cgaatgagca accgcaaatg
     3841 gttcgaaagg gaattcgatc tcagttttgg tcaggacgag tatactgcta tttatgagcg
     3901 gctaagaacg gcaccggaaa agttgaggca ggtggtggcc ggtttgccgg aggaggtgct
     3961 tttgcaaaag cctgatggca agtggtcggt taaggagcat gcggggcatc tttccgtgct
     4021 cgagccattg tggcgtatcc ggttccagga catccgggag gggaagccgg tgctgaccac
     4081 ggccgaccta aacaacactg cgacatcgaa tgccgggttt aatgactacc cggttgcagc
     4141 attagtggag caatttgcga aggagcggag tgccacatta tccctgctga acagcatcga
     4201 tgtgctcgat gaggagcata cgagtctgca tcccaggttg caacaaccga tgcggatcat
     4261 cgacctggcc tattttgtcg cggagcatga tgagcatcat tttgcggcga tgagggagat
     4321 gagccggtag tttgcgggtg gtggtgcggc gtgggaggag atttaagaat gcgtaccttt
     4381 ataaaaaaaa gttatggtag catttttagg catggggct
//
LOCUS       DATGFG010001048         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_333411, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001048 DATGFG010000000
VERSION     DATGFG010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333411"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VKQ52_19960"
     CDS             <1..847
                     /locus_tag="VKQ52_19960"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ89544.1"
                     /translation="CLPRDQRQTLDALNKIYVNSATGASVPLSNVADIGFLSSPTSIR
                     HYDKDRYTTVTAFVRSGYNTTRLTEGILKKLDSMRFPAGATYKAAGEVESKEESFGGL
                     GPIILITVFGILGILILEFKTFRGTLIVLSVVPLGIIGAVLILLATGNTFSFVAVIGI
                     IALVGIEVKNSILLVDYTDQLRKGGMGLDEAIQEAGETRFVPIILTTLTAIGGLIPLV
                     AENNPLYSPLALVIIGGLLSSTILTRVVTPVLYKLLAPRISQDPGPETAGGASLLPVP
                     VVASA"
     gene            complement(954..2123)
                     /locus_tag="VKQ52_19965"
     CDS             complement(954..2123)
                     /locus_tag="VKQ52_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89545.1"
                     /translation="MQLIEVIHPDTAKEFIQVNVELNRSIPGYIRPIDKEINEVFDKS
                     INKAFRHGEVTRWILKDDNGRLIGRIAAFTNKKYRNKGDEVPVGGVGFFDCIDNQDAA
                     DMLLDVARHWLGQRGMEAMDGPINFGERDKWWGLLVEGFHEPLWSMNFNPPYYKELLE
                     NYGFKPFFHQLCFGMDPMAPLSPKIAQRHDALAVDPLYNARSIEKKNLEKYAADFVEV
                     YNAAYAGHGGLKEMRKDQAVQLFKKMKPLLDEDIAWFAYYGERPIAMFLNIPELNQYY
                     KHFNGKVGLLQKLQFLWLRSRGACRHFTGIIFALIPEFQGKGVDAYIIQKLANAVQPA
                     RFYVHYEMQWIGDFNPKMINVAKSLGDSHVSRKLTTYRYLFDRTREFKRHPMVGA"
BASE COUNT          500 a          664 c          613 g          545 t
ORIGIN      
        1 ctgtctgccc cgcgaccagc ggcagaccct cgatgcgctg aataagatct atgtcaattc
       61 ggccaccggc gcatccgttc cactgagtaa tgtcgccgat atcgggttcc tgagcagtcc
      121 caccagcatc cggcactatg acaaggaccg ctatacgacg gtcaccgcct ttgttcgcag
      181 cgggtataat acgacccggc tcaccgaagg tatcctgaag aaactggact ccatgcgttt
      241 ccccgccggg gcgacctata aggcggcagg cgaagtggag agcaaggagg aaagctttgg
      301 cggtctcggc cccatcatcc tgataactgt ttttggtatc ctggggatcc tgatcctcga
      361 attcaaaacc ttccgcggaa cgcttatcgt gctgtccgtc gtgccgctgg gcatcatcgg
      421 ggcggtgctg atcctgctgg ccaccggcaa taccttctcc ttcgtggccg tcatcgggat
      481 catcgccctt gtcggcatcg aggtcaagaa ctcgattttg ctggtggatt ataccgatca
      541 gttacgcaag ggcggtatgg gtctcgacga ggccatccag gaggcgggcg aaacgcgttt
      601 cgtccccatc attctcacga ctcttacggc gatcggcgga ctcattccgc tggtggcgga
      661 gaataatccc ctgtattcgc cgctggcgct cgtcatcatt ggcggattgc tcagctcgac
      721 cattctcacc cgggtggtga cgccggtgct gtataaactg cttgccccgc gcatcagcca
      781 ggatcccggg ccggagacgg ccggaggcgc aagcctgttg ccggtgcctg tggtggcgtc
      841 ggcgtaatag aaacgctttt agccgaaaaa gagacgcaaa agagttggaa tgagcgagaa
      901 cggataggat ttaatcactg acaaaggccg gcgcaagccg gcctttacgg ttcttatgcc
      961 cccaccatcg gatgccgctt gaactccctg gtccggtcga acaaataccg gtaggtcgtc
     1021 agcttccggc tgacatggct gtcgccgagg gacttcgcca cattgatcat cttggggttg
     1081 aaatccccga tccattgcat ctcgtaatgg acatagaacc tggcgggctg cactgcattg
     1141 gccagcttct ggatgatata ggcatcgacc cccttcccct ggaactcggg gatcagcgcg
     1201 aagatgatcc cggtgaagtg ccggcaggcg ccccggctcc ggagccagag gaactgcagc
     1261 ttttgcagca gaccgacctt cccgttaaaa tgcttatagt attggttgag ttcggggata
     1321 ttcaggaaca tcgcgatcgg ccgttcgcca taataggcga accaggcaat atcctcgtcg
     1381 agcagcggct tcatcttttt gaacaattgc acggcctggt ccttccgcat ctccttcagg
     1441 cccccatggc cggcataggc ggcattgtag acttctacga agtcggcggc atatttttcg
     1501 aggttcttct tttcaatgct ccgggcgtta tagagcggat cgacggcaag ggcatcgtga
     1561 cgctgcgcga tcttcggact cagcggcgcc atggggtcca tgccaaaaca caactggtgg
     1621 aaaaagggtt tgaagccata gttctccaac agctccttat aataaggagg gttgaagttc
     1681 atggaccaaa gcggttcgtg aaagccttcg accagcaatc cccaccactt atcccgttcg
     1741 ccgaaattga tcggtccatc catcgcctcc attccccgct gtccgagcca atgcctggcc
     1801 acgtccagca gcatatccgc agcatcctga ttatcaatgc aatcaaaaaa accgaccccg
     1861 ccaaccggca cctcgtctcc cttattccgg tattttttat tggtaaaagc ggcgatccgg
     1921 ccgatgagcc ggccgttgtc atcttttagt atccagcggg tcacttcccc gtggcggaag
     1981 gccttgttga tgctcttatc gaatacctcg ttgatttcct tgtcgatagg gcgaatatag
     2041 cccggaatac tccggttgag ttctacattc acctgtataa attcttttgc cgtgtcggga
     2101 tggataactt ctatcagttg cataacctat cggattgtat cagggccccc gtgtacaacc
     2161 ggactccgtc cggttaggac aaatatagta tttttaactt tccccctgtc cccttacctt
     2221 tgcgccaaaa ttgacaaact aatcaataat atggcaagta acggtaagat caaacagatc
     2281 atcggcgcgg ttatcgatgt gcagtttgaa ggcaaactcc ct
//
LOCUS       DATGFG010001049         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_60327, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001049 DATGFG010000000
VERSION     DATGFG010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60327"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2866
                     /locus_tag="VKQ52_19970"
     CDS             <1..>2866
                     /locus_tag="VKQ52_19970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLZ89546.1"
                     /translation="LEGDLLREKLYYWKQRLEGVEPLQLPADYTRPAVQSTRGAGLQF
                     KIDAALAQSLQRLSLQEGASLYMMLLAVFQVLLHRYSGQEDIAVGSPIAGRQQQETEG
                     LIGFFINTLVLRSRVSDGLSFRELLGQVKEATLGAFAHQEVPFEKVVEVLSVERDRSR
                     GPLVDVKFALHNMPPSGELDLGPKVNLKIEEHGAVTAQVAISVDIMESAEGLWMGITY
                     CTDLYKGETMRRMAGHYIELLHSVVADAGESIGALRMLGDDEERQLREGFNATDRTWG
                     GGRTVLELFAGHVRQHPGRIAVRSEVRDRVELWPSVAEFFVYDGLLYRAMLQDHRRNR
                     WYAEALSERVHDKVVVEIGPGPEAILSRLSIEAGARKVYAIELLEDAYRKAKRFVEEA
                     GLADRIMLIHGDAMKVELPELADVCISELVGPIGGSEGAAVILNNARRLLKQGGGMIP
                     GRNLTRIAPVSLPDYFAARPGFSETGGVYVDKVFDYLGHPADIRLRVRNFPHRYLLSE
                     PGVFEDLDFRQGLVAEQSVHRISFDIGRDDLLQGFLVWLTLESDGTVLDTLEHEYCWL
                     PVFVPVFYPGVAVAQGDRLEVEVERRLSANGINPDFHIRGYLRQAGGRLDFDYSLPHE
                     PERFREHAFYQQVFDEHGHGVVNGKEKVYETMSYAELHRQSDRLSGWLRQQGMAGGVV
                     AVWQDSSIWTVSTLLAILKASALYLPIDPQWPMERIASIWEESKPAGCITARSYQEQV
                     EALAPAGAVLMSRERWEHYGEEGAEAIIAEPPGEAQGAYIIYTSGSTGRPKGVVIAHR
                     GLLNLTQAQIEGFGIGIGDRVLQFASLSFDASISEIFTTLCAGAELVVYDRQTFSAEE
                     LFGVLRDQNIGIVTLPPSVLRILPSEGLTALHTVVSAGEACDGEIVKQWRPGRRFINA
                     YGPTEYTVCTTMNEVGWPEPLPVSIGRPIANTRLWVLGGHG"
BASE COUNT          566 a          667 c         1027 g          606 t
ORIGIN      
        1 ctggagggcg atctgctgcg ggagaaatta tactattgga agcagcggct ggagggagtg
       61 gagccattgc agctgccggc cgactatacg cgtcctgcgg tgcagtctac gcggggcgcc
      121 ggcctgcaat tcaagatcga cgcggcgttg gcccagtcgc tgcagcgatt atccctgcag
      181 gagggtgcga gtctgtacat gatgttgctg gcggtgttcc aggtgctgtt acaccggtac
      241 agcggacagg aagatatcgc tgtgggaagc ccgatcgccg gccggcagca gcaggagaca
      301 gagggattga tcggtttttt tatcaataca ctggtgctgc ggagccgggt aagcgatggg
      361 ctgtcgtttc gggaattgct gggccaggtg aaagaagcga cgttgggtgc ttttgcgcat
      421 caggaggtgc cgttcgagaa ggtggtggag gtattgtcag ttgagcggga caggagccgg
      481 ggcccactgg ttgatgtaaa attcgcatta cacaatatgc ctccatcggg tgagctggat
      541 ctgggaccga aggtgaatct gaagattgag gagcatgggg cggtgacagc acaggtggcc
      601 atcagtgtgg atatcatgga atcagcggaa gggttgtgga tggggatcac ctattgcacc
      661 gatctgtata aaggggagac gatgcgccgg atggcgggtc actatataga gctgttgcat
      721 tcggtggtgg ctgatgccgg tgagtccatc ggcgcactgc ggatgctggg agatgacgaa
      781 gagcgtcagc tcagggaagg cttcaatgcc actgaccgta catggggggg aggacggacg
      841 gtgctggaat tgttcgccgg gcatgtcagg caacatcccg ggcggatcgc ggtgcggagt
      901 gaagtgcgcg atcgcgtgga gctttggcct tcggtggctg aattcttcgt atatgatggg
      961 ctgttatacc gggctatgct tcaggatcat cgtcgaaacc ggtggtatgc ggaggcgttg
     1021 agcgagcggg tacatgacaa ggtggtagtg gagatcgggc cgggaccgga ggccattctt
     1081 tcgcggctat cgatagaggc cggcgcccgg aaagtctacg ccatcgaact gttggaagat
     1141 gcctaccgga aggcgaaacg gtttgtagaa gaggccgggc tggccgacag gatcatgctg
     1201 atccatggcg atgctatgaa ggtagagttg cccgagctgg ctgacgtgtg tatatcagag
     1261 ctggtgggcc cgataggggg aagcgaaggg gctgcggtga tcctgaacaa cgcgcggcga
     1321 ctcctgaaac aaggcggcgg gatgatcccc gggcggaacc tgacgcggat cgcgccggtg
     1381 agcctgccgg attattttgc tgcgcgcccc gggttctccg aaacgggagg cgtttatgtc
     1441 gacaaggtct tcgattacct gggtcatcca gccgatatcc ggctacgcgt aaggaatttc
     1501 ccgcaccgct atctattgtc ggagccgggt gttttcgaag acctggattt ccgccagggg
     1561 ttggtggccg agcagagcgt ccaccggata agctttgaca ttgggcggga tgatcttttg
     1621 caggggttcc tggtctggtt gacgctggag tcggatggga cggtgttgga cacgctggag
     1681 catgagtatt gctggctgcc cgtgttcgtg ccggtattct accccggggt ggctgtagca
     1741 cagggcgatc gcctggaagt tgaggtagag cggcgattaa gcgccaacgg catcaaccct
     1801 gatttccata ttcgtggcta cctgcggcag gcgggaggcc gattggattt cgactattcg
     1861 ctgccgcatg aaccggagcg attccgggag catgcctttt atcaacaggt attcgatgag
     1921 catggccacg gggttgtaaa tggcaaggag aaggtctatg agacgatgag ctatgcggag
     1981 cttcaccggc aatcagatcg tctgtcgggg tggttgcggc aacaggggat ggccggcggg
     2041 gtcgttgcgg tatggcagga cagctcaata tggacggtca gcactttgct ggcgattttg
     2101 aaagcctcgg cactgtattt acctatagat ccgcagtggc cgatggaacg gatcgcatcc
     2161 atctgggaag agagcaagcc ggcaggctgc atcactgccc ggagctacca ggagcaggtg
     2221 gaggcgctgg cgcctgcagg ggccgtgctg atgagccgtg agcgttggga acattatggg
     2281 gaggaaggag cggaagcgat catagcggag cctccggggg aagcgcaggg agcctatatc
     2341 atctacacgt cgggatcgac agggcggccc aagggggtgg tgatcgctca ccgcgggctg
     2401 ctgaacctta cgcaggcaca gatagaaggg tttggcatcg gcatcggcga ccgggttttg
     2461 caatttgctt cgctgagctt cgatgcttcg atctcggaga tcttcaccac cttgtgtgcc
     2521 ggggcggagc tggtcgtcta cgaccggcag acattttccg ccgaagagct gtttggagtg
     2581 ttgcgtgacc aaaacatcgg gattgtgact ttgccgccct cggtgctacg gatcttgcca
     2641 tccgaggggc taacggcttt gcatacggtc gtctcggccg gggaagcctg cgacggggag
     2701 atcgtgaagc aatggcgacc gggacgccgc tttattaatg catatggtcc tacggaatat
     2761 actgtttgta cgacgatgaa cgaggtgggg tggcccgaac cgttgccggt gtcgatcggc
     2821 aggccgatcg ccaatacccg gctgtgggtg ttgggcggcc atggag
//
LOCUS       DATGFG010001050         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_289867, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001050 DATGFG010000000
VERSION     DATGFG010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289867"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VKQ52_19975"
     CDS             complement(<1..1308)
                     /locus_tag="VKQ52_19975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89547.1"
                     /translation="MFRNYFTVAWRNLIKSKWYSLINTLGLSIGMAVALLIGLWIWDE
                     VSYDHYYPNHARIAQLMTTQTFNGHTGTGRAVSIPAGHELQGKYGADLPRVALASWNS
                     LHVLAVGDKKITDGGMYVEPQFTEIVPVKLLSGQRDALKDPAAILLGQSMARNLFGKE
                     DVVGQVVKLDNKAPLKVAGVFQDPPQNSSFFDTKYLLSWGQYELQNEWIKRNETQWGN
                     HSWQCFVLLSPHTDAARVTGKIKDLPKAHNKDGNESLVLQPMDNFHLYSDFTDGKVSG
                     GRIRFVWLFGIIGAFVLLLACINFMNLSTARSEKRAKEVGIRKTVGSLRVQLIGQFLC
                     ESVVVALLALVLAILWVVISLPFFNSLSNKDMHLPWAQPLFWLIVIGFTLVTGLLAGS
                     YPAFYLSGFEPIKVLKGSFRAGRYASLPRKVLVVIQFTVSVALI"
     gene            1400..>2471
                     /locus_tag="VKQ52_19980"
     CDS             1400..>2471
                     /locus_tag="VKQ52_19980"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89548.1"
                     /translation="MLRNFLKISFRHLIRKKAFFAINIFGLTAGLVSCILIGLYVSDE
                     LSFDRFHANACRIVRMTMEFQKGNSSTPNHVATCGTKPGPQFKRLFPIVENYVRTMEF
                     PVLVSAGTETFTENRFLYADPSFFSIFSFPLLKGDPAALSVKSNIVITASAAKKYLGT
                     TDALGKTLRVNDRKDFRVSAVAQDPPGNSQLHFDFIVPFNNLDASKSEIWWTANYITY
                     LLLTRPDQAAPLEKQIAVYMRTPDIRKEAELEGSDYLTYHLEPLTSVHLHSPLPGFEP
                     NGNIVYVYVLILIALLILVIACFNYTNLAIALSADRTGEIGIRKVLGAGKRQLFTQFA
                     GESVLITALALILAVGISIVLLP"
BASE COUNT          576 a          822 c          575 g          498 t
ORIGIN      
        1 gatcaacgcc accgacaccg taaactggat caccaccagc accttccgcg gcagcgaagc
       61 ataccggccc gcccggaatg accccttcag caccttgatc ggctcaaaac ccgaaagata
      121 gaacgcggga taactccccg ccagcagccc cgtaacaagg gtaaacccaa taacgatcaa
      181 ccagaacagt ggctgcgccc agggtagatg catatcctta ttggaaagcg aattgaagaa
      241 gggcaacgag atcaccaccc agaggatcgc caatacaagc gccagcaacg ccaccaccac
      301 cgactcgcaa aggaattgcc cgataagctg cacccgcagc gacccgaccg tcttccggat
      361 ccccacctcc ttggcccgct tctcgctccg cgccgtcgac aggttcatga aattgataca
      421 ggccagcaac aggacaaacg ccccgatgat cccgaaaagc cagacaaacc ggatccggcc
      481 gccggatacc ttgccgtccg taaaatcact gtagagatgg aaattatcca tgggctgcag
      541 caccagcgac tcattcccat ccttattatg cgccttggga agatccttga ttttccccgt
      601 cacccgcgcg gcatcggtat gcggcgacaa tagcacgaaa cattgccagc tgtggttccc
      661 ccattgcgtc tcgttccgct tgatccactc attctgcagc tcatattgcc cccaggaaag
      721 gaggtatttc gtatcgaaaa aagaactgtt ctgcggcggg tcctggaaca cccccgccac
      781 cttcaacggc gccttgttat ccagcttgac cacctgcccc accacatcct ccttcccaaa
      841 caggttcctc gccatcgact gacccagcag gatcgcggcc ggatccttca acgcatcccg
      901 ctgcccgctc agtaacttca ccggcacgat ctccgtaaat tgaggctcca catacatccc
      961 cccgtccgtg atcttcttgt ctccaacagc gaggacatgc agggaattcc agctcgcaag
     1021 ggccaccctc ggcagatcgg ccccatactt gccctgcagc tcatgacccg ccgggatact
     1081 caccgccctg ccggtgcccg tatgcccgtt aaaggtctgg gtcgtcatca gttgggcgat
     1141 gcgcgcatga ttgggatagt agtggtcata ggatacctcg tcccagatcc acagcccgat
     1201 cagcagggct acggccatcc cgatggagag cccaagggta ttgatcaggg aataccactt
     1261 acttttaata aggttacgcc aggcaacggt gaaatagtta cgaaacatac gacgggtttg
     1321 ccgctaagga cggatccgcc ggcaaaaatg ttgcatcgta gaaaccctgt atatttgaaa
     1381 cataccaaaa acactcctta tgctccggaa tttcctcaaa atatccttcc gccacctcat
     1441 ccggaaaaag gccttttttg ccatcaatat cttcgggctc accgccggcc tggtgagttg
     1501 tatcctcatc ggtctctatg tctcggacga actgagcttc gaccgctttc acgccaatgc
     1561 ctgccgcatt gtccggatga cgatggagtt ccagaaaggc aacagttcca ctcccaacca
     1621 tgtcgccacc tgcgggacca aacccgggcc ccagttcaaa cggctcttcc ccatcgtgga
     1681 gaactatgtc cggacgatgg agttccccgt cctcgtcagc gcgggaacgg aaacatttac
     1741 agagaaccgg ttcctctatg ccgacccttc cttcttttcg atcttctcct tccccttgct
     1801 gaaaggtgac ccggccgccc tctccgtcaa gagcaatatc gtcatcaccg cctcggccgc
     1861 taaaaaatac ctcggtacga cggacgccct tggaaaaacc ctaagggtca acgatagaaa
     1921 ggatttccgg gtcagcgccg tagcccagga cccgcccggc aactcccaac tgcatttcga
     1981 cttcattgtc cctttcaaca acctggacgc ttcgaagtcg gaaatatggt ggacggccaa
     2041 ctatatcacc tacctcctgt tgacccgtcc cgaccaggcc gccccgctgg aaaagcagat
     2101 cgccgtctat atgcggacgc cggatatccg caaggaagcc gaactcgaag gaagtgatta
     2161 cctcacctac cacctcgagc cgctgaccag cgtccacctg cactctcccc tgccggggtt
     2221 cgagccgaac ggcaatatcg tctatgtcta cgtgctgatc ctcatcgccc tgctgatcct
     2281 cgtcatcgcc tgcttcaact ataccaatct cgccatcgcc ctgtcggcgg atcgtacggg
     2341 ggagatcggc attcggaaag tgttgggagc agggaaaaga caattgttca cccaattcgc
     2401 cggcgaatcc gtccttatca cggccctggc cctgatcctc gcggtgggta tcagcatcgt
     2461 ccttttgccc c
//
LOCUS       DATGFG010001051         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_638204, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001051 DATGFG010000000
VERSION     DATGFG010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638204"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VKQ52_19985"
     CDS             complement(<1..289)
                     /locus_tag="VKQ52_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89549.1"
                     /translation="MTRKLFFLERLLYGDGRAPFHAVFPIRISGAPALQNLRRGLEKV
                     QSKHPLLQSRIVQDERGVPHFVADGQAAPIGIRIVDRKADDDWIHESMQEWV"
     gene            complement(359..1567)
                     /locus_tag="VKQ52_19990"
     CDS             complement(359..1567)
                     /locus_tag="VKQ52_19990"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HLZ89550.1"
                     /translation="MAKFMFVVPPFFGHISPTLSVGADLLARGHSVLWVGLRELPAKH
                     LPAGGRWIVPPATSALHGEEILRILKRQDDGVNLSGMEVLKLAVEETYLPFCRFLMEG
                     LPEVTDQFEPDVIISDCITFAGGICAFKKGIPFVTSTPVAPDVMKSAMDMPRVLQWHR
                     NLVMGLQREFGVLTDEAVVLSNELNIVFTSREFAGVPAPPPQMKFVGPVKGRPDTTPF
                     DWEKFRKLPSPRIFISLGTLLRDIRPVYFQRMVEAFGHQPLGVIAATDPGILPEWPDN
                     FLVQDYVPQSLLLSKVNAVICHGGFNTVNDAIFNQLPILITPITYDHFHNAALVVAAG
                     CGIQLRYKRLRTDDLKSAVWSLLENEKYAIAARKHKNFFLSAGGNAKAVEYLEEFAGA
                     RRVMVMGAVG"
     gene            complement(1579..>2179)
                     /locus_tag="VKQ52_19995"
     CDS             complement(1579..>2179)
                     /locus_tag="VKQ52_19995"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HLZ89551.1"
                     /translation="ALRDEPLSVIVVSDPALFATWPPNFMVRSRVPQLELLPYLEAVV
                     CHAGHNTVCESLIHGLPLVVVPIAYDQSHVASRVTSTESGIRLNFKRFKAAHLKQAVR
                     DVLGDARYRDAARRIQASFAKAGGAERAAGLLEKLAAGAGMKGIGVREAGIERGMRED
                     GMKEAEVREAGIERGMCEAGMKEDGMEGVGMREAGMQTI"
BASE COUNT          501 a          701 c          519 g          458 t
ORIGIN      
        1 tgacccactc ctgcatcgat tcatgtatcc agtcatcgtc cgctttccgg tcgacgatgc
       61 ggatgccaat cggggcagcc tgaccatcag caacgaaatg aggtaccccc cgttcatcct
      121 gcacgatacg ggattgcagc aaaggatgtt tggactgcac cttttcaagc ccgcggcgga
      181 gattttgaag ggccggggct ccgctgatcc tgatcgggaa tacggcatga aaaggagcac
      241 gaccgtcgcc gtataaaagt ctttcgagga aaaataattt tcttgtcata aacacaggtt
      301 tatcctttat ccctgccact cgcatgcgcg gatcatcgtc atccgacgcc acccatcatc
      361 atcccacggc gcccatcacc atcacccgcc gggccccggc aaattcttcc aggtattcaa
      421 cggctttcgc attcccgccc gccgaaagga aaaaattctt gtgcttccgc gcagcgatcg
      481 catatttctc attttcaagc aggctccata cagccgattt aagatcgtcc gtccgcagcc
      541 gtttatatct caactggatg ccacagcccg ctgccacgac gagggcggca ttgtggaaat
      601 gatcgtaggt gatgggcgtt atcaggatag gcagctggtt aaaaatagcg tcattgacgg
      661 tattgaaccc gccatgacag atgacggcat tcaccttcga caggagcagg gattgaggga
      721 catagtcctg gacgagaaag ttatcaggcc actcgggcag tatcccgggg tcggtcgccg
      781 ctataacacc cagcggctga tggccaaacg cctcgaccat cctttgaaag tagaccggcc
      841 ggatatctct cagtaaggtg cccaaagaaa taaagatccg gggactcggc aatttcctaa
      901 atttctccca atcgaaagga gtagtatccg gccggccttt caccggaccg acaaatttca
      961 tttgcggcgg cggcgccgga actccggcga attccctgga cgtaaaaaca atgttcaact
     1021 cgttcgatag gaccaccgcc tcatcggtaa gaaccccgaa ttcccgctga agccccatca
     1081 ccaggtttcg atgccactgg agaacccttg gcatatccat cgcgcttttc atgacatcgg
     1141 gcgccaccgg cgtgctggtg acaaaaggga tccccttttt gaacgcgcag atcccaccgg
     1201 cgaaggtgat gcaatcgctg atgatgacgt cgggctcaaa ctggtccgtt acttccggaa
     1261 gcccttccat caaaaaccgg cagaagggca gataggtctc ctcaacagcc agtttcaaca
     1321 cttccatccc ggacaagttg accccatcat cctgcctttt aaggatgcgc agtatctcct
     1381 ctccatgcag ggcggatgtt gcaggaggca cgatccatct gccgccggcg ggaagatgtt
     1441 tggcaggcag ctcccgcagc cctacccata agaccgaatg ccctctcgcc aacaggtccg
     1501 cccccacgct gagtgtcggg ctgatatgcc cgaaaaacgg tggaacaacg aacatgaatt
     1561 ttgccatata ttcctttttt aaatggtttg catcccagcc tcacgcatcc caaccccttc
     1621 catcccgtcc tctttcatcc cagcctcaca catcccgcgc tctatcccgg cttcgcgcac
     1681 ctcagcctcc ttcatcccgt cctcacgcat cccccgttct atcccggctt cgcgcacccc
     1741 aatccctttc atcccggccc cggcagccag tttttccaac aaccccgcag ccctctccgc
     1801 cccgccggct tttgcaaagg acgcctggat acgcctggcc gcatccctgt accgggcatc
     1861 gcccagcaca tccctcaccg cctgtttcag atgggcggcc ttaaagcgtt taaaattgag
     1921 tcgtatcccg ctttccgtac tggtaacccg gctcgccaca tgggactgat cataggcaat
     1981 ggggacaacc accaacggca ggccgtggat caggctctca cacaccgtat tatgtcccgc
     2041 atgacagacc acagcctcca ggtacggcag caattccaat tgcggaaccc ggctgcgaac
     2101 cataaaattt ggcggccagg tggcaaaaag agccggatcg gaaaccacta tgacggacaa
     2161 cggctcatcc ctcagcgct
//
LOCUS       DATGFG010001052         7121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_518205, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001052 DATGFG010000000
VERSION     DATGFG010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518205"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VKQ52_20000"
     CDS             complement(<1..315)
                     /locus_tag="VKQ52_20000"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HLZ89552.1"
                     /translation="MKYIDLIKRLDNMSNYKRREIITNWLEDHQVKYWKHEYATGVNL
                     VVDLGTSSRKIGVSSHFDRVKGSPGANDNSSAMAVCLDMIRKHQENGNMNIGLRIFFF
                     DEE"
     gene            complement(432..656)
                     /locus_tag="VKQ52_20005"
     CDS             complement(432..656)
                     /locus_tag="VKQ52_20005"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HLZ89553.1"
                     /translation="MENNQTPGKFITRTRAVNADGTRIAIQTALLCSLCRLIPFNAFS
                     ALGDPSYPWHDRLSRTLVIDEKVTQLPPWS"
     gene            complement(686..2050)
                     /locus_tag="VKQ52_20010"
     CDS             complement(686..2050)
                     /locus_tag="VKQ52_20010"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HLZ89554.1"
                     /translation="MSTWKEYQEKNKDRFLNELLELLRIPSVSARSEHRPDMLKCAEA
                     VKQRLLDAGVDKAEIYPTEGHPVVYGEKIIDKSKPTVLVYGHYDVQPADPLDLWHSGP
                     FEPVIKDGKIYARGSADDKGQFYMHVKALETMIQTKTLPTNVKFIIEGEEEIGSPNLA
                     KFVSTHTDLLKADVILISDTAMLSLDTPSIDIGVRGLSYIEVEVTGPNRDLHSGVYGG
                     AVANPITMLAKMIASCHDGNNHITIPGFYDGVVEATPAERKLMAAAPYDEAEYKKDLG
                     VTELWGEKGYTTNERTGIRPTLELNGIWGGYTGEGAKTVLPSKAYAKISARLVPNQSA
                     KVITEKLLHYFRQIAPPGVTVKAEEHHGGEPYMTPIDSKAYKAAAKAIEATFGKAPIP
                     VRGGGSIPICSLFEKELGIKIVFMGFGLDSDNLHSPNEKFELANFYKGIETIPYFHQF
                     FAEG"
     gene            complement(2134..2580)
                     /locus_tag="VKQ52_20015"
     CDS             complement(2134..2580)
                     /locus_tag="VKQ52_20015"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HLZ89555.1"
                     /translation="MNNYTVSATQQHILDDLGVYLVRASAGQRLANLLIDLICFCLFW
                     AIPGLLSPEFWTLFFIPLLPAVAFGIYMSLMEIAMKGKTIGKLVTGTRAVQVDGSPIN
                     STIAWQRGFSRIVPFEAFSALGGDTHPWHDGWTGTVVVVEKDSRGI"
     gene            complement(2737..3558)
                     /locus_tag="VKQ52_20020"
     CDS             complement(2737..3558)
                     /locus_tag="VKQ52_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89556.1"
                     /translation="MSDFSSVLGLSGATLQRLGDAYEEYAAALGVQIRQQRERQEPDM
                     LLDEKELLSGSCYVLAAMYRVILDPGEAIPLFGFAADIYRRQNNTFWKLLAICGLNRE
                     LLFGQEVSKVEGNPQEFFYELLRQYYLFNSGDENALGRMLEYASSSPALPAQPVGNPP
                     VALREFISFIRGDIEKAYYADEPGENKDWTVSLRVLLQRTAEQLQLLQSHRLPPTNET
                     VLLPFEPDILATLLGFCVRNRNRADLRAFAEQESRHGSPAVFLVGMALEMIDASL"
     gene            3771..4427
                     /locus_tag="VKQ52_20025"
     CDS             3771..4427
                     /locus_tag="VKQ52_20025"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HLZ89557.1"
                     /translation="MTKDRVGALSLLLTFIAGYTDVTTFVAADKLFSAHITGNFIVLA
                     YDLIRGADRSAYMKLLAFPVFVIAAMAAAWYDRAGGRPGRLLRLEGALLLLAAGLSGP
                     LHSNFAAAMLIVAAMAVQNAFWRLYPALSWGMTTVMTGNVTTATVSFVQGFWARPRNT
                     EKLTQSRRILAMAGLFLSGCLCGGFLAARFGLVVVAGPGIIVLLASRLTGPAHSLKKP
                     "
     gene            4469..5362
                     /locus_tag="VKQ52_20030"
     CDS             4469..5362
                     /locus_tag="VKQ52_20030"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ89558.1"
                     /translation="MAKQKIPVYNIANLAARPLDPDDCMIEPFGTYLGRHSPHLHNPH
                     RHSFYHLVCFTSGKGSHSIDFTRFPVKAGQVYFMTPGQVHSWNFEGQPGGYVINFSEG
                     FLQSFLLNPQYLDRFSFFSGNAADSVVQLVSPIREQVAALLEQMVGQYRDRVHTDADL
                     LRAELLQLFLLVQSGIGARRPAASAALTQKQQLLTTLRKLMDQHYKTLRRPSEYAGLL
                     YITPNHLNTLCQDLLGRSAGEVIRDRVLLEAKRLLTNAGMTATEIAYELNFQDNSYFN
                     RFFKKYEGVTPEEFRRKNRVK"
     gene            complement(5437..6702)
                     /locus_tag="VKQ52_20035"
     CDS             complement(5437..6702)
                     /locus_tag="VKQ52_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HLZ89559.1"
                     /translation="MYRILHSLLLFSAIALSVHLASAQDQAQPVKGVRFEHELSWSAV
                     QARAMTGNKYIFVDCFTTWCGPCRYMRTTIFPQEEAGNFFNDKFVSVEVQLDTTAKDN
                     DHVKGWYADAHQIMTQYGIHAFPTYLIFAPNGRIVHRIVGGRETAREFIGVAREAFDT
                     SRQYYTQLEQFKAGRGDSAFLHRLADQSLGLYDFQDGTPVFKAWLATQHSMYTPTAMH
                     LMENYTQHSSDIGFPVFLHHAAEADKILGAGKAERLVINTLYREYARPQLQSAGADGP
                     DWKAIQTAVAAKYPAQAGEVTARSKVLYYESKKDWPHFQTVIVAYMQKYGAHATPEEL
                     NSYAWDVFSNCPDMSCVADALDWSKRSFKSEANPTFMDTYANILYKMGKKDDAIAWEE
                     KALNLSSGNDRANYQSTIDKMRKGEKTWN"
     gene            complement(6820..>7121)
                     /locus_tag="VKQ52_20040"
     CDS             complement(6820..>7121)
                     /locus_tag="VKQ52_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89560.1"
                     /translation="HLTVPWYLNVLEAHIEVDALTNLIRSRFGESMEFFVHTDACFDF
                     SCRICTKEECNVRKHAFEERVEWTLANVVANKRHVSPRVSKDQLHLEFPEATTQA"
BASE COUNT         1427 a         1983 c         2137 g         1574 t
ORIGIN      
        1 ctcctcatcg aaaaagaata ttcgtaaccc gatgttcata tttccatttt cctgatgctt
       61 cctgatcata tcgaggcaaa cagccatcgc ggagctgttg tcattggcgc cgggggagcc
      121 cttaaccctg tcgaaatgtg agcttacccc gattttcctg ctgcttgtcc ccagatcaac
      181 caccaggttc acgccggttg catattcgtg cttccaatac ttcacctggt ggtcctctaa
      241 ccagttcgtg atgatctccc ttcgtttgta atttgacatg ttgtcaagcc gtttgataag
      301 atcgatatat ttcatgccgt gattttttag caattatagg attgtgcaga cgagaggccc
      361 gcttcttagg aagctgatag tagtgtggat ggagcttaga aaggagggac aaatgtaggt
      421 atttttttga ctcaactcca cgggggcaac tgcgtcacct tctcatcaat caccagcgtc
      481 ctcgacagcc ggtcatgcca gggataactc ggatctccca gcgcggagaa ggcattgaag
      541 gggatgaggc ggcagaggct gcagagcagc gccgtctgga tggcgatccg ggtgccgtcg
      601 gcattgacgg cccgggtgcg ggtgatgaat ttccccgggg tttggttgtt ttccatgatt
      661 cgggtctacc tgtccggata agggattacc cctccgcgaa gaattgatga aaatacggga
      721 tcgtctcgat ccccttatag aaattcgcga gctcgaactt ctcgtttggc gagtgcaggt
      781 tatcgctgtc cagcccaaaa cccataaaga cgatcttgat acccaactct ttttcaaaaa
      841 gagagcagat ggggatactg ccgccgccgc gaacgggaat gggagccttg ccaaaagtgg
      901 cttcgatggc ctttgccgcc gccttgtaag ccttgctgtc gatcggcgtc atatagggct
      961 cgccgccatg atgctcttcg gccttgaccg tgacgccggg cggcgcgatc tgccggaaat
     1021 aatgcagcag tttttcagtg atgaccttcg ccgattgatt gggaaccagc cgggccgaga
     1081 tcttggcgta tgccttgctt ggcaacaccg tcttcgcccc ctcgccggta tagccgcccc
     1141 agatgccgtt cagctcgagc gtaggccgga tgccggtacg ctcattggtg gtatatcctt
     1201 tttcgcccca caattccgtt accccgaggt ccttcttgta ttccgcctca tcgtacggtg
     1261 cggcagccat tagcttccgc tcggccggcg tcgcctcgac gaccccgtca tagaaaccgg
     1321 gaatcgtaat atgattgttc ccgtcatggc aggaggcgat catcttcgcc agcatcgtta
     1381 tcgggttcgc cacggcgccg ccataaaccc cgctgtgcag gtcgcggttg gggccggtga
     1441 cctccacctc gatatagctg agaccccgca ccccgatgtc gatagacggc gtgtcgaggc
     1501 tgagcatggc cgtatcgctg atcaggatga cgtcggcctt gaggaggtcc gtatgtgtgc
     1561 tcacgaattt cgccaggttg gggctgccga tctcctcttc gccttcgatg atgaatttga
     1621 cgttggtggg cagggtcttt gtctggatca tcgtctcgag ggcctttaca tgcatataga
     1681 actggccctt gtcatcggcg gaaccccggg catagatctt cccgtctttt atcacgggct
     1741 cgaagggacc actatgccac agatcgagcg ggtctgcggg ctggacatca taatgcccat
     1801 acaccagtac ggtcggttta cttttatcga tgatcttctc cccatacacg acagggtggc
     1861 cctccgtggg atagatctcc gctttgtcaa caccggcatc cagcaggcgc tgttttaccg
     1921 cttcggcgca tttcagcata tccggccggt gttcgctccg cgcgctgacg gagggaatgc
     1981 ggagcaactc gagcagttcg ttcagaaaac gatccttatt tttttcctgg tattctttcc
     2041 atgtcgacat gacgcaaata tagggaattt gccggccgga cgctccgaag gggccgtggg
     2101 ctgacgggtg gccggagatg gtagatttcg cggctagatc ccccgggaat ccttttcgac
     2161 gacgacaacg gtgccggtcc acccatcatg ccagggatgg gtatccccgc caagcgccga
     2221 gaaggcttcg aagggcacga tccggctgaa cccgcgctgc caggcgatgg tggagttgat
     2281 ggggctgccg tcgacttgta cggcccttgt gccggtgacc agctttccga tggtcttccc
     2341 cttcatggcg atctccatca gcgacatata gatgccaaag gcgacggcgg gtaagagggg
     2401 aataaagaac agtgtccaaa actccgggga cagcaggccg gggatggccc agaagaggca
     2461 gaaacagata agatcgataa gaagatttgc caggcgttga ccggcgctgg cgcgtacaag
     2521 atacacgcca agatcgtcca ggatatgctg ctgggtcgca gatacggtat aattattcaa
     2581 aggtgtcagt ttattcgagt gcccgcaaaa taaggaaaga ttttcggatg gacaaacggg
     2641 tgggttacgg gaacgtcgat gggtgactca cgggaacgtc gatggatggc tcacggggaa
     2701 cgtcaatgga tggatacggg aatgtcgatg gttggctcac agggatgcat cgatcatctc
     2761 aagcgccatc cctacaagga agaccgcggg actgccatga cgcgactcct gttccgcgaa
     2821 ggcccgcaga tcggccctgt ttcggttgcg gacacaaaaa ccaagaaggg tggcgaggat
     2881 atcgggctca aaaggcaata acacggtctc atttgtcggg gggagccggt ggctttgcaa
     2941 caattgcaat tgttcggctg tccgttgcag taagacccgc aggctgaccg tccagtcttt
     3001 gttctcgccg ggctcgtcgg cataataggc cttctcgatg tctccgcgga taaaggagat
     3061 gaactcccgc agggcgacag ggggattgcc cacgggttgg gccggcaggg cgggagagga
     3121 tgaggcatat tccagcatcc ggcccagcgc gttctcgtcc ccgctgttga aaagataata
     3181 ttgtcgaagc agctcgtaga agaattcctg gggattaccc tccaccttgc tgacctcctg
     3241 gccaaaaagc aattcccggt tgagcccgca gatggccagc agtttccaga aagtgttgtt
     3301 ctgccggcga tagatgtccg cggcaaagcc gaaaagcggg atggcttcac cgggatccag
     3361 gatgacccgg tacatcgccg ccagcacata gcaactgccg ctgagcagct ctttttcatc
     3421 gaggagcatg tccggctcct ggcgttcgcg ttgttgtcgt atctgcaccc ccaatgccgc
     3481 ggcatactcc tcataggcat cgccaagccg ttgcagcgtt gcacccgaaa gacccagtac
     3541 ggaggagaaa tcactcatga tcacaaggtt tcgcgggcaa aaataacgtg gttggcaaga
     3601 accatccggg ggtaaaaaca cccatttttg ggtaggcgac cggcggataa tgccccggcg
     3661 gggcgaaggg gcgaaggggc gaacacgcga gcaggcgaag ggacgagcag gcgaaggggc
     3721 gagaggcatg cggcgtcgga ggtaaagcag tatcttgggt aaaaagtagt atgacgaaag
     3781 atcgggttgg cgctcttagc ctcctcctga cgtttattgc cgggtatacg gatgtgacaa
     3841 cctttgtcgc cgcggacaag ctcttctctg cccatataac aggtaatttt attgtcctgg
     3901 cctacgatct gatccgtggc gccgaccgta gcgcgtatat gaaattgctg gccttccctg
     3961 tttttgtgat cgcggcgatg gcggccgcgt ggtatgaccg tgccggcggg cggccgggcc
     4021 ggctgctgcg gctggaagga gcgctgcttt tgctagcggc cgggctgagt ggtccgttgc
     4081 attcgaattt tgcggcggcg atgctgatcg ttgcggccat ggcggtgcag aacgcgtttt
     4141 ggcgattgta tccggcgctc tcctggggta tgacgacggt gatgaccggc aatgtcacta
     4201 cggcgaccgt ttcctttgtg caggggtttt gggcaagacc ccggaatacg gaaaagctaa
     4261 cccaaagcag gcggatcctc gccatggcgg ggttgttcct ttccggctgt ctctgcggcg
     4321 gcttcctggc ggcgcggttt gggttggtcg tggtcgcggg ccccgggata atcgtgctgc
     4381 tggcatcccg gcttaccggc cccgcccatt cgctgaaaaa gccataattt gcccggcgaa
     4441 ccggccgaac aaccgggtcg gaccaaccat ggccaagcag aagatcccgg tgtacaatat
     4501 tgccaacctg gcggcacggc cgctcgaccc ggatgattgc atgatcgaac cctttggcac
     4561 ctatctcggc cgtcattccc cgcatctgca taacccgcac cgccacagct tctatcatct
     4621 cgtctgcttc accagcggca agggatcgca cagcatcgat tttacccggt tcccggtcaa
     4681 ggcaggccag gtctatttta tgacccccgg ccaggtacac agctggaatt tcgaggggca
     4741 gcccggcggc tatgtcatca atttttcgga agggttcctc caaagctttt tgctcaaccc
     4801 ccagtacctc gaccgctttt ctttcttcag cggcaatgcc gccgattccg tcgtacaact
     4861 ggtttcgcca attcgggagc aggtggcggc cttgctggaa cagatggtcg ggcaataccg
     4921 cgatcgtgtc cataccgatg ccgacctgct gcgcgctgag ctgctacagc tattcctcct
     4981 cgtccagtcg ggcatcgggg cccggcggcc cgccgcttcg gccgcgctca cacaaaaaca
     5041 acagctgctg accaccctgc gcaaactgat ggaccagcat tacaagaccc ttcgccggcc
     5101 cagcgaatat gccggcctgc tctatatcac tcccaaccac ctcaacaccc tctgccagga
     5161 cctgcttggc cgctccgccg gcgaggtcat ccgcgacagg gtgctgctcg aggccaaacg
     5221 gctgctcacc aatgcgggca tgacggcgac ggagatcgcc tatgagttga atttccagga
     5281 taacagttat ttcaaccgct tctttaagaa gtatgaggga gtgacgcccg aggagttccg
     5341 aagaaagaat agggtgaagt gacccctgtc gctccgaggg tggtaaccct ccgccgctca
     5401 cgccggcccc gccccgccaa agagatggct cacgcctcaa ttccaggtct tctcgccctt
     5461 ccgcatcttg tcgatcgtgg attggtaatt ggcgcggtcg tttccggagc tgaggttgag
     5521 ggccttctct tcccaggcga tggcgtcgtc cttcttgccc atcttgtaga ggatgttggc
     5581 ataggtgtcc atgaacgtgg gattagcttc gctcttgaac gaccgcttgc tccagtcgag
     5641 ggcgtcggcg acacaactca tgtcggggca gttggaaaaa acatcccagg catagctgtt
     5701 cagttcctct ggcgtggcat gtgcgccgta tttctgcata taggcgacga tgacggtctg
     5761 gaaatgcggc cagtccttct tgctctcata atacaacacc ttgctgcggg ccgtcacttc
     5821 cccggcctgg gcgggatatt tggcggcgac ggcggtttga atcgccttcc agtcggggcc
     5881 atcggcgcca gccgactgaa gttgcggccg ggcatattcg cggtacaggg tattgatgac
     5941 cagccgctcc gccttgccgg cgccgaggat tttgtccgct tccgccgcat ggtgcaggaa
     6001 gaccgggaag ccgatatcgc tgctgtgctg ggtataattt tccatcagat gcattgccgt
     6061 tggggtgtac atgctgtgtt gcgtggccag ccaggctttg aagacggggg tcccgtcctg
     6121 gaaatcgtag aggccaagcg actggtcggc gaggcggtgc aggaaggcgc tgtcgccgcg
     6181 gccggccttg aactgttcca gctgcgtgta atattggcgg ctggtgtcga aggcttcgcg
     6241 ggccacgccg atgaattccc ttgcggtctc gcggccgccg acgatgcgat gcacgattct
     6301 gccgttgggc gcgaagatga gataggtggg gaaggcgtgg atgccgtatt gggtcatgat
     6361 ctggtgggca tcggcatacc agcccttgac gtggtcgtta tcctttgccg tcgtgtcgag
     6421 ctgcacttcg acgctgacga atttgtcgtt gaagaaattc cccgcctctt cctgggggaa
     6481 gatggtggtg cgcatgtacc ggcagggacc gcaccaggtg gtgaagcagt ccacgaaaat
     6541 atacttgttc ccggtcatgg ccctggcctg aacggccgac caggaaagct cgtgctcgaa
     6601 gcggacccct ttcaccggtt gcgcctggtc ctgtgcagag gccaggtgga cagacaaggc
     6661 aatcgccgaa aagagcagta gggagtggag aatgcggtac atggcgcttt tttttacaag
     6721 gtaggaagcg ccgccaaaaa aggcaaaaaa ttgggatgag cggggccgcg ggaatgggat
     6781 ccgcccggcc ggcgaagggc tgctgcggca gagcgccgtt cacgcctgcg tcgtcgcttc
     6841 agggaactcc aggtgcagct gatccttcga caccctcgga ctcacatgcc gcttattggc
     6901 gaccacattc gcgagggtcc attccacccg ctcttcgaag gcatgcttgc ggacattgca
     6961 ttcctccttg gtgcagatgc ggcaggagaa gtcgaaacag gcgtcggtgt ggacgaagaa
     7021 ttccatggat tcaccaaagc ggctgcgtat caggttagta agggcatcca cctcgatatg
     7081 ggcctccagc acgttgagat accagggcac ggtgagatga c
//
LOCUS       DATGFG010001053         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_917363, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001053 DATGFG010000000
VERSION     DATGFG010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917363"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VKQ52_20045"
     CDS             <1..492
                     /locus_tag="VKQ52_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89561.1"
                     /translation="LQSAVEQLGKLEWGIRVKGEDGEKKDEDKDKKEDAYSLLHRYGN
                     RQREEVINSVPGSIYKVELDNSHPLAFGYPDHYYTLKEDDKVYEFLKDGGWNVGIVRK
                     DGYVSGFTGVRAKEKIKDGLVFGVQGMGRGTVIYMADDPLFRSFWENGKLLFCNAVFL
                     VGQ"
     gene            506..>2312
                     /locus_tag="VKQ52_20050"
     CDS             506..>2312
                     /locus_tag="VKQ52_20050"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HLZ89562.1"
                     /translation="MKRVFVFLFAAACGVFGGAAFGQAPETVTSAEIFLRLNKLNVLG
                     TVLYMAAHPDDENSRLIAYMAKERLFRTAYLSMTRGDGGQNLIGDEQGVELGLIRTQE
                     MLAARRVDGAEQFFSRAFDFGFSKSTDEALRTWDKEKVLSDAVWVIRKFQPDVIITRF
                     PPDSRAGHGHHSASALLAHEAFDAAADPTKFPEQFKYGVKPWQAKRVLWNTFNFGGAN
                     TTSEDQLKIDVGGFNPILGKSYGELAAISRTNHKSQGAALTPARGQSTEYFTLVAGEG
                     AHSDPMDGIETSWRRVEGGERIEGMVKQLIQDFSLAHPERSVPGLAAVYKAISELKDG
                     YWKTQKLREVRQLIGDCSGLWLEAVTRAPYAVQGDSVAVTVTINNRLHASLSLVDVAL
                     VGADNGFMVAMKQDLEGDKNYTFSRSIYVSPDKPVSQPYWLAEEMSPGSFNVKDQRQI
                     GDPQGPPAYEVAFRLSAGGQEIVLTRPVQFKYTDAVKGELYEPLTVLPQATAQFDPEL
                     VLFADGEEKGFRVEATDRTGRMGLPKMSLTPTPGVTVGPPSGLSWLAKPVGSGSSGTV
                     TASLLFDHGGKRDTALQLKTIAYEHIPRIDYFRQAK"
BASE COUNT          494 a          539 c          780 g          499 t
ORIGIN      
        1 ttgcagagcg cggtggagca attgggcaag ctggaatggg ggatccgggt gaaaggcgag
       61 gatggcgaaa agaaggacga ggacaaggac aagaaggagg acgcttattc gctcctgcac
      121 cggtacggca accgtcagcg ggaggaggtg atcaacagcg ttcccggttc tatttataaa
      181 gtggagctgg acaatagcca tccgcttgcc tttggctatc ccgatcatta ttataccctg
      241 aaggaggatg ataaggtgta tgagttcctg aaagacggtg gctggaatgt ggggatcgtc
      301 aggaaggacg gttatgtcag cggttttacg ggtgtcaggg ccaaggagaa gataaaggat
      361 gggcttgtct ttggggtgca ggggatgggc agggggacgg tcatttatat ggccgatgat
      421 ccgttgttcc gcagtttctg ggagaatggg aagctgttat tttgcaatgc agtttttttg
      481 gttggtcaat aaaagatact tgtacatgaa gcgcgtattt gtttttttgt ttgcggcggc
      541 atgcggggtt tttggcgggg cggcctttgg ccaggcgccg gagacggtga cctcggcgga
      601 gatctttctg cgattgaata aattgaatgt cctgggcacc gtgttgtata tggctgctca
      661 ccccgatgat gagaactcgc ggttgatcgc ctatatggcg aaggagcgat tgttccggac
      721 ggcgtacctg tcgatgacca ggggcgatgg cgggcagaat ctcatcgggg atgagcaggg
      781 agtcgagctg gggctgatcc gcacccagga gatgctggcc gcccgcaggg tggatggggc
      841 cgagcagttc ttttcgcggg cctttgattt tggattctcc aaatcgacgg atgaggcgct
      901 caggacctgg gataaggaaa aggttcttag cgatgccgtc tgggtgatcc gcaaatttca
      961 accggatgtg atcatcacac ggttcccacc ggacagcagg gccgggcatg gtcatcattc
     1021 ggcctcggcg ctactggccc acgaggcctt cgacgcggcg gctgacccga ccaaattccc
     1081 ggagcaattc aaatatggcg taaaaccctg gcaggcgaag cgggttttat ggaacacctt
     1141 caattttggc ggcgccaata ctaccagcga ggaccagctc aagatcgatg tcggtgggtt
     1201 caaccctatt ttgggaaaga gctatggtga actggctgcg ataagccgga ccaatcataa
     1261 gagccagggt gcggcgttga cgccggcccg tggacaatcg acggaatatt ttaccctcgt
     1321 ggccggtgag ggtgcgcata gcgacccgat ggatgggata gagaccagtt ggcgccgggt
     1381 ggagggagga gaaaggatcg aagggatggt gaagcagctt atccaggatt tttcccttgc
     1441 gcaccccgag agatcggtgc cggggcttgc cgccgtctac aaggcgatca gtgagctgaa
     1501 ggatggatat tggaagacgc aaaagctaag agaggtacgg cagcttatcg gcgattgcag
     1561 cggtctgtgg ctagaggcgg tgacgcgcgc tccttatgcc gtacaggggg attcggttgc
     1621 ggtgaccgtt acgatcaaca accggctgca cgcatccctt tcccttgtcg atgttgcgct
     1681 ggtgggcgcc gacaatggat ttatggttgc gatgaagcag gaccttgaag gagataagaa
     1741 ctataccttt tcgcggagta tctatgtatc acccgacaaa ccggtgagcc agccctactg
     1801 gcttgcggaa gagatgtcgc cggggtcttt taatgtaaag gatcagcggc agatcggcga
     1861 cccgcagggg cctcctgcgt acgaggttgc cttccggctg tcagccgggg gccaggagat
     1921 cgttcttacg cgaccggtgc agtttaaata taccgatgcc gtaaaaggcg aactatacga
     1981 accgttgacc gtgctgccgc aggctacggc gcaattcgac ccggagctgg tgctttttgc
     2041 ggatggcgag gagaagggct tccgggtcga ggcgaccgac aggacgggac gcatggggtt
     2101 gccgaagatg agcctgacac cgacgccggg agtgaccgtc gggcctcctt cgggtctttc
     2161 ctggctggcg aagccggtcg gctccggctc cagcggaacg gtgacggcca gcctgctttt
     2221 tgatcatggc ggcaagcggg ataccgcgct gcagttgaag accatcgcct atgagcatat
     2281 tccccggatc gattatttcc ggcaggcgaa gg
//
LOCUS       DATGFG010001054         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1666890, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001054 DATGFG010000000
VERSION     DATGFG010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666890"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VKQ52_20055"
     CDS             complement(<1..436)
                     /locus_tag="VKQ52_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89563.1"
                     /translation="MKRALLPLFLLLFIAVELFAQSKRVNGTADYFPPRGEWVHRSPG
                     ALGLDSGVLAAAIRFARDNESKAPRDQWLAQAESFGRAEPFDEPMGPMKPRGEPTGLI
                     LYKGYIVAEWGEPARVDMTHSVTKSFLSSVIGLAVDQGLIRSV"
     gene            complement(474..1082)
                     /locus_tag="VKQ52_20060"
     CDS             complement(474..1082)
                     /locus_tag="VKQ52_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89564.1"
                     /translation="MALVSCFSVAAQQNTPVEEEHAQLLTGKKRCLIAYAGKDHSFTL
                     ETPVHSAKPSDVPGFITIDKQIVQATVVPVDKGIDLHTLSAAREREVLLKYMEYELDY
                     YRKKLKQHYSHLQTEWVTLQGKQFLVWYFDMPENYKMVSRQVYISTLFFDQVMDLNAP
                     VFKSKDSDWGRARGILIRLAGTMKTFDKRLDLEELGRKLNKG"
     gene            1271..>2218
                     /locus_tag="VKQ52_20065"
     CDS             1271..>2218
                     /locus_tag="VKQ52_20065"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HLZ89565.1"
                     /translation="MKRIKNAIWMAALAAMTTSPALAQRRNHPTLPAATDTSKPKPPV
                     FPGFPMPKQGPKPYKEVITDKAISHFGLFTVHKVDERWYFEIPDSLLGREFMAITRFS
                     KTAAGGGIYGGELANQQTLEWDKGPAHSLFLRVITLVSMADTTSKIFKAVKNSSVDPI
                     AAAFEIKAYGKDSSSVVIDVTDYFKGDNLIVSIPQGIKSRMRLGGLASDRSFIEHINT
                     YPINTEVRSVKTFSSGGGMGSPFGISFSSPTDAAGAVTLELNTSFILLPAVPMAKRLS
                     DTRVGYFADDYVVYSDEQQKIENQEFIVRWRLEPRAEDRE"
BASE COUNT          517 a          706 c          543 g          452 t
ORIGIN      
        1 gcacactccg gatcagcccc tggtcgacgg ccagcccgat caccgacgag agaaaactct
       61 tggtaacact gtgcgtcata tccacccgcg cgggctcacc ccactcggca acgatatacc
      121 ccttgtagag gatcagcccc gtcggctccc cacgcggctt catcgggccc atgggctcgt
      181 cgaagggctc ggcccgccca aaactctccg cctgcgccag ccactggtcc cgcggcgcct
      241 tcgattcatt gtcccgggca aaccggatcg ccgccgccag gacaccggaa tccagcccca
      301 gcgcgcccgg agaacgatgc acccactccc cgcgcggcgg gaaatagtcg gcagtcccat
      361 taactctttt ggattgtgca aaaagctcaa cagcgataaa aagcagtaag aatagcggta
      421 gaagggctcg tttcatagcc cttccaatat acgaaagatt ggatcatccg gtcctacccc
      481 ttattcaatt ttcttcccaa ctcctccagg tccagccgct tgtcgaacgt cttcatcgtc
      541 cccgcaagcc ggatcaggat cccccgcgcc cttccccagt cagaatcttt agacttgaat
      601 accggcgcgt tcaggtccat cacctggtca aagaacaaag tggaaatata cacctgccgg
      661 ctcaccattt tataattctc cggcatatcg aaataccaaa ccaggaattg cttcccctgc
      721 agggtcaccc actccgtttg cagatgactg taatgttgtt tgagcttttt ccggtaatag
      781 tccaattcat actccatata tttcagcaac acctcccgtt cccgggccgc cgatagggta
      841 tggagatcga tacccttgtc caccggcacg accgtagcct ggacgatctg cttgtcgatc
      901 gtgataaatc cggggacatc cgaaggcttg gccgagtgca ccggcgtctc caacgtaaag
      961 gaatgatcct tgccggcata ggcgatcagg catcgttttt taccggttag gagctgggca
     1021 tgttcctctt cgacaggggt attttgctgg gcggctacgg aaaaacacga gacaagggcc
     1081 aaggccggaa ggattccggc tggccgaagg ccaattttta gcgctggagc gcggaaaggc
     1141 caaatttttg tccaggcctg gaagcgctcc aaagccgaaa gtagtccggc cggcagggtg
     1201 ggggctagtt tcaatttttt tgcctattta acgaattaac agggaaaaat cgtatagttt
     1261 tacgcccctc atgaagcgaa taaagaatgc catttggatg gcggcgctgg ccgcaatgac
     1321 gacctcccct gctcttgccc aacgcagaaa tcatcccacc cttcccgccg ccaccgatac
     1381 ctcaaaaccg aagcccccgg tcttccccgg cttcccgatg ccaaaacagg gcccaaaacc
     1441 ctataaagag gtgatcaccg ataaggccat ctcccatttt ggcctgttca ccgtccataa
     1501 ggtggacgaa cgatggtatt tcgagatccc cgactccctc ctgggccggg aattcatggc
     1561 catcacccgc tttagcaaaa cggccgccgg cggcggcatt tatggcggcg agctcgccaa
     1621 ccagcaaacc ctcgaatggg ataaaggccc cgcacattcc ctgttcctcc gcgtcattac
     1681 cctcgtcagc atggccgaca ccaccagtaa gatcttcaag gccgtaaaaa attcaagcgt
     1741 agaccccatc gcggccgcct tcgagataaa agcttatggc aaggactcct caagtgtggt
     1801 catcgacgtg acggactatt ttaaaggcga caacctcatc gtcagcatcc cccagggcat
     1861 aaaaagccgg atgcgcctcg gcggcctggc ctccgacaga agcttcatcg aacacatcaa
     1921 cacctatccc atcaacaccg aagtaagaag cgtaaagacc ttctcttccg gcgggggcat
     1981 gggctcgccc ttcggtattt ctttctcttc cccgacggac gccgccggcg ccgtcacgct
     2041 ggaattgaac acctccttca tcctgctgcc cgccgtcccc atggcaaaac ggctctccga
     2101 cacgcgcgtc ggctattttg cggatgacta tgtcgtctat agcgacgaac aacagaagat
     2161 agagaaccag gagttcatcg ttcgctggcg cctcgagcca agggccgaag accgcgaa
//
LOCUS       DATGFG010001055         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_744647, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001055 DATGFG010000000
VERSION     DATGFG010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744647"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2272..>2645
                     /locus_tag="VKQ52_20070"
     CDS             2272..>2645
                     /locus_tag="VKQ52_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89566.1"
                     /translation="MKKKAIASQQSLFDDHPELTSAPALDQEEHDFVFDILDYLQSPI
                     IVYDPSWADMLPPRYIDLVPIQRLAAGIRREPLATEAEVILYIYTWSMVRPIHDHHWV
                     NIYTYLVGKTIPKGSEENLRET"
BASE COUNT          661 a          739 c          629 g          616 t
ORIGIN      
        1 ccggcagcgg gagaccagga cgtcggcaac gattcgacaa ttgctgaaaa gccggcgtct
       61 ttccaatgat aacctccatc ggccaactgt actcaacgtt taggcagtga gtgtgcccgg
      121 ctccttttac gcatacgaaa cagcggaggt gagcgcttcc aacaccccac ggcaaaccac
      181 ccccagcggg agtgattgga tcccggtgcc cggtccctgt gcattttccc ggggttggtt
      241 cctgtcggct ggtttatggg ggacggcgca agtgccttga tgggacggcc gtcgcatgct
      301 atggtatctc caaagaaaat ccgtaagatc cgagtgggat tgctacatag ggcagacagg
      361 gcaaaaatgc cagcctacag caagattcat gcataaaagt tagtatcttc ccttacgata
      421 attgtaaagc ctttcctaaa gctgctgcag atgactagcc acacctatcc tattaccatt
      481 atctatcggt tggacaactc aaaactgaca gttgccttga ctgccgatgt cgaagtacat
      541 gaaccggaaa catactatag tataaaaaat atccgaccca aggcggaata tagcaggtcc
      601 gtcttaccgg acatcgtcat aaaaaaagtc gacggcaggt ggattcaccg ggatagtgaa
      661 cgggaatcgc atttcagcat tcaagtaggg gaagccatcg atgcccacca accagccccc
      721 gatgcgggac cggtcatttc ttccgatagc caaagcgcca catcctgaat ctcattccga
      781 gatccttttc aaatgtgcag gcaacaagat tttcgccgtg aatcgactct ttccattcgc
      841 cacgaagggg ccggcctggg ttcgcgtgtg cgtgacgaag ggtttaccgt ctgaggcatt
      901 atcctttact tcgcctgcgg gcacttccat caacaccttg ccttcaaatg atccctgtag
      961 gtatagaaca atttcatgcg ccgcaacagg ccagtagaca gtttgtatgc tttgagttca
     1021 tccgcatgtc tccttgcaaa gaggctatcg aggcggcatc gagtttgtcg ctttttcccg
     1081 cttgatcgaa agcccgttgt taataacgcc tcccggggcc atgcaatact caattttctg
     1141 ctccgccggg tattcccaaa acggctggct aaactccggt atgcgccatg caaatcagcc
     1201 actctgattt tttatctttt taagccatta cgcccccctt cctgaaaccc attgttgtat
     1261 tctctatcct tgatgtagtc tccttttatt tacttccaat gaatgatctg ttaatccata
     1321 actcggacac atccacttgg gcgaatagtg aattcatgaa tgagaggggg gattctcaaa
     1381 aaaaggggta aatgcaccga cgcctttcct gggcttatag tcgcggcatt ctagttaagt
     1441 tgcatgaacc cttgtaaaaa ccaatggcag acgaatgcaa cggttagatc cttaatctgg
     1501 cgacacgcta agtttacagc cattgctatc gatgcccgcc tcgtatttca gcatacgatt
     1561 acgctgttgc aaaactgtcg tccttatttt aatgtcttcg tacacagata cttaaacacc
     1621 aagatttgct gtcttaaccc gctctttccg ttgacagggt aatggagcag gttttctttt
     1681 ttggagacca gcttttgcac tactagcaaa tgtagaggcg acgctccgtt catcgaccac
     1741 ctacagatgg accggtaggg gttacgcgag ggggggagcc gaaaaaaaga acccgctcgc
     1801 cgttcggccg gtaacacggt cgccgctcgc gcggcgaagg aagagcccca ggaaccccga
     1861 agagcctgtt aacaaatgga ttaacaatga agggcaaaag cagcacaacc tgaggcatcc
     1921 ctttctttcg ccggtcgcta aatggttcgg cacgggtcgt cagcggcaga tcctgcaccg
     1981 gcggtagtac cgtccgacgc ggaaagactg ctcgctccgc gtcgcacctt tccgcatcgg
     2041 accaagcagc cgcgccccac cgcattggcg ccggcgaaga taaagcggat gcctccaccg
     2101 gtgtgctttg cttttttgcc cttcatattg ttaatccgtt tgtgggggat gggggaagag
     2161 tggggaagaa caaggaagat caccggtcta ccccgaaaga caacgaacct accctttctc
     2221 ctcccccgca cggatacgcg aagggcaata catcctttaa atgaattcag catgaaaaag
     2281 aaagcaattg cctcccagca aagcctcttc gacgatcatc cggagctaac gagcgcgccg
     2341 gcactcgacc aggaagaaca cgattttgta tttgacatcc tcgactacct ccagtcgccg
     2401 attattgtct acgatccctc ctgggcagac atgctaccac cccgctacat agacctcgta
     2461 cccatccaac gactggcagc cggtatccgc agagagcccc tggccaccga agccgaagtc
     2521 atcctctaca tctacacctg gtccatggtc cggcccatcc acgatcacca ttgggtcaac
     2581 atttacacct acctcgtggg caaaaccatc ccgaaagggt ccgaagaaaa tctccgtgaa
     2641 acatt
//
LOCUS       DATGFG010001056         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1430055, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001056 DATGFG010000000
VERSION     DATGFG010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430055"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VKQ52_20075"
     CDS             complement(<1..1025)
                     /locus_tag="VKQ52_20075"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HLZ89567.1"
                     /translation="MKANPAFSAVFCLAFGFCLFACSKHDSKPTLSVSTNSLLLDSVI
                     GAVDSFSVSSNVSWKLTVSPAAATSWLQVSQTTGSNSAIIRLTITGRNTNVTGPLTIT
                     VAAVSGSLSPQTITLDQQGALQVSNTSLTLKGTPVTDTLAINTGLAWKATASAGWIHL
                     DTTQGAGSYRLKVSADTNRTGAPQSGTVTLTPLNNTSAAAITVNVTQSAYYAILSFSP
                     LSGAVGSTITLNGYFPSSFSVTMNNSSAAAIVSHSTTQIVCTVPGDATGGYLFVNIPS
                     VFPSIVSTQQFTVTSAWKSLSDNTAGLPGYNPGSLVYTYNGALYTGFGATGSQTIYRL
                     DTTTFHWV"
     gene            1242..1766
                     /locus_tag="VKQ52_20080"
     CDS             1242..1766
                     /locus_tag="VKQ52_20080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ89568.1"
                     /translation="MNPADLLRLIQDNKGILYKISSSYCRNAGDREDLMQEMIYQLWR
                     SAPRYDKNQKFSTWMYRIALNVAISFYRKTSRSGVTVELDAEAQFSDESGDEVLTERI
                     EVLQRFIRNLNELDKALMILYLEERPYREMAEILGVSETNVATKLSRIKGRLKQEFGL
                     GRTAPGPDNLNLMV"
     gene            1763..2410
                     /locus_tag="VKQ52_20085"
     CDS             1763..2410
                     /locus_tag="VKQ52_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89569.1"
                     /translation="MITDEFQQLWKAYDAKLERSLQLNQRLWEDMQKQKATSALRPLL
                     RTGVVGIITGVLWAALMGFLAYRLRTQPVMAISFGVFFICTVIGIGAYIRDITVIGGV
                     DYAGDIVYAQEKLARLRGSMIRTFRVIWLQLPFWSTFFVSNAMLREGMWQTLLIEVPV
                     FLLLTAVALFLYRNVTVENARRKKWVAAFLRGSGARSVARAISLLDEIETFKREG"
     gene            complement(2503..>3107)
                     /locus_tag="VKQ52_20090"
     CDS             complement(2503..>3107)
                     /locus_tag="VKQ52_20090"
                     /inference="COORDINATES: protein motif:HMM:NF024847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLZ89570.1"
                     /translation="LFKIGKTQDLRRRLASYRTHLPVVFRIVRQYPAAKMDTLEESLH
                     IVFQHRRVQGEWFRLTNDDLTICDNIARNFALVKLQRQVRTVPEINYSDNPLLQVMEA
                     NEKYLRDYSHIADDLKLGLSTEEIVQLHEGGISKTTIATVRRLLDYQTPNSEFLSQWF
                     DVVSELEAGATENELLKKYGGQVNRNTIKMIKRILRNQLY"
BASE COUNT          661 a          794 c          977 g          675 t
ORIGIN      
        1 acccagtgaa aggttgtcgt atccagccgg tagatcgttt gagaacccgt ggcgccaaag
       61 ccggtgtaaa gcgcgccgtt ataagtgtat acaagggagc cggggttgta accgggaagg
      121 cctgccgtat tgtcggacag ggacttccag gcgcttgtca ccgtaaattg ttgcgtggaa
      181 acaattgacg ggaaaacgct gggaatattg acgaacagat acccccctgt tgcatcgccc
      241 ggcaccgtgc agacgatctg tgtggtgcta tggctcacga tggcggcagc agagctgttg
      301 ttcatagtga cgctgaaact ggatgggaaa tagccattga gcgtgatagt agaacccacg
      361 gcgccactta gcggagaaaa actcaggatg gcatagtagg cgctctgcgt tacattgacc
      421 gtaatcgcag cggcgctggt gttgttgagc ggcgtcagcg taaccgtccc gctctggggg
      481 gcgcccgtac ggttggtgtc ggcgcttacc ttcagccggt agctccccgc gccctgagtc
      541 gtatccaggt ggatccagcc ggcggaagcg gtcgctttcc aggccagccc ggtgttgata
      601 gccagggtgt cggtaaccgg tgttcccttc agggtgagac tggtattgct cacctgcagc
      661 gccccctgct ggtcgagggt gatcgtttgc ggcgaaagac tgccgcttac ggccgccacg
      721 gtgatggtca acggaccagt gacgtttgta ttgcggccgg tgatcgtcag ccggataatg
      781 gcgctgttgg agccggtagt ctgactcacc tgtaaccagc tggtggccgc ggcgggagag
      841 accgtgagtt tccaggatac attggaagag acgctgaagc tatccacggc gccaatgaca
      901 ctgtccagca acaggctgtt ggtggaaacg gaaagggttg gtttggagtc gtgcttactg
      961 caggcgaaaa ggcaaaagcc gaaggcaaga caaaagacag cggagaaggc cggattcgct
     1021 ttcataacgt gattaggttt taggttggcg catctaagat agccgatttc ccattagaat
     1081 ggaagccccg cttcggcgtt gattgccaat aaaatgcggg ttgcacggct atgagcggat
     1141 aaacacgctc cggggccttt ccaacacgct gataaatgcc ggcgggggaa atttttttcc
     1201 cggaacggtg attttccaga ccttcctgcg aataatagag tatgaacccg gccgacctgc
     1261 tccgactgat acaggacaat aagggcatct tgtataagat cagctcctcc tattgccgca
     1321 atgccggcga tcgggaagac ctgatgcagg agatgatcta ccagttgtgg cggtcggcgc
     1381 cccgatatga caaaaaccaa aaattctcta cctggatgta ccggattgca ttaaacgtgg
     1441 cgatctcctt ctaccggaag acaagccgca gcggggtcac cgtcgaactg gatgcggaag
     1501 cgcagttctc cgatgaaagc ggcgacgagg tgctgactga gcggatcgag gtgttgcagc
     1561 ggttcatccg caacctcaat gagctggaca aggcgctgat gatcctctat ctcgaggagc
     1621 ggccttaccg ggagatggcg gagatcctcg gggtctcgga gacgaatgtg gctacgaagc
     1681 tgagccggat aaagggccgg ttgaaacagg aatttgggct gggccgaacg gcgcctggtc
     1741 cggacaattt aaatttaatg gtatgatcac agatgaattt cagcaactat ggaaggcgta
     1801 tgacgcgaaa ctggagcgct cgctgcaact gaaccagcgc ctttgggaag acatgcaaaa
     1861 acagaaggcg acgtcggcgc tccgcccctt gctgcgcacg ggtgtcgtcg gcatcatcac
     1921 gggggtccta tgggctgcgc tgatgggatt tcttgcttac cgccttcgga cccagccggt
     1981 gatggccatc tcgttcggcg tattctttat ctgtacggtc atcggcatcg gggcatatat
     2041 ccgggatatc accgtgatcg gcggggtcga ttatgccggc gatatcgtat atgcgcagga
     2101 gaaactcgcc cggctgcggg gctccatgat ccggaccttc agggtgatat ggctgcagct
     2161 gccgttctgg agtacgttct ttgtcagcaa tgcgatgctc cgggagggga tgtggcagac
     2221 cctgctgatc gaagtgccgg tatttctctt gctaacagcg gtggcgctct tcctctaccg
     2281 gaatgtcacc gtggagaatg cgcggcggaa gaaatgggtg gccgcgttcc ttcgcgggtc
     2341 gggcgccagg tcggtggcca gggcgatctc cctgctggac gagatcgaaa ccttcaaacg
     2401 ggaagggtag ggccctaacg gcagctccct caatacagcc cccggcagca gcggcccgac
     2461 aacacccccc cgcggctgcc cgacagcccc cccacggcgg cctcaataca actggtttcg
     2521 caggatccgt tttatcattt tgatcgtatt ccggttcacc tgcccaccat attttttcaa
     2581 aagctcattt tcggtggcgc cggcttccag ctcggacacg acgtcgaacc attggctgag
     2641 gaattcggaa ttgggggtct ggtagtcgag cagccggcga accgtggcaa tggtagtctt
     2701 gctgatcccg ccctcgtgga gctggacgat ctcttcggtg ctgaggccaa gtttgagatc
     2761 gtcggcgata tgcgaatagt cacgaagata tttttcattt gcctccatca cctgaaggag
     2821 cgggttgtcg ctatagttga tctccggaac agttcttacc tggcgttgta atttaacgag
     2881 tgcaaaattg cgggcgatat tgtcgcaaat ggtcaggtcg tcgttggtga ggcggaacca
     2941 ttcgccctgg acgcgccggt gctggaagac gatatgaaga ctttcttcga gggtgtccat
     3001 ctttgcggca gggtattgcc ggacgatgcg gaagacgacg gggagatggg tgcggtagga
     3061 ggcgagacgg cggcgaaggt cctgcgtctt gccgatcttg aacagat
//
LOCUS       DATGFG010001057         5212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1686179, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001057 DATGFG010000000
VERSION     DATGFG010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686179"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..625
                     /locus_tag="VKQ52_20095"
     CDS             233..625
                     /locus_tag="VKQ52_20095"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLZ89571.1"
                     /translation="MNNSNEEQIREALNAHWHASAIGDANAEHAIYHDDAICDYPQSG
                     ERILGRSNLQALRSHHPGKPSGFSVRRIVGKGDLWITEYTITYQGRPAYTVSIMEFRD
                     GKVAHETQYFADPFEAPAWRSQWVQRMP"
     gene            762..1322
                     /locus_tag="VKQ52_20100"
     CDS             762..1322
                     /locus_tag="VKQ52_20100"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLZ89572.1"
                     /translation="MRNLIFAINTTLDGCCDHTQFNPDEEMMDYFTRLTRDADTFLYG
                     RKTYQLMVPYWPDIAKDHSGQTKADIEFAQAFDAVDKIIVFSQTLDSPEGKNTRIVHT
                     ILPDEIRKLKQEQGKNILTGGVAIPTQLAGLGLIDEYRFVVHPIVAGEGRRLFEGISL
                     QEKLRLKLVDSTVFKSGCVALRYLKQ"
     gene            complement(1353..2771)
                     /locus_tag="VKQ52_20105"
     CDS             complement(1353..2771)
                     /locus_tag="VKQ52_20105"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ89573.1"
                     /translation="MYLLIRHLFIRPGRLVSIRYSRLVLALLIVPTAMAQAQFTIQNG
                     AVITTNGHAVITLQDIDLIIDGSISQQAGDGFWVFTGSGDNSISGSSSPLMDKLEIAK
                     TGVAGIILSQDITIAGGIEFNSGLIDLNGNHIFLQPGAMLNGENESSRIKSDNGGYVE
                     TTSRLNAPVIFNPGNLGAWITSSQDLGSTIIRRGHASQTSSGGVGNSILRYYDILPAN
                     NTALNATLRINYLDAELNGLDESALVLWKSTDTLHWTNIGYTARDATANYVEASGIAD
                     FSRWTLSTVNNPLPVRLTSFIVECITGLARINWKTAQEQNSSRFDVEKSMDGIQWQAI
                     GHLAAAGNSNGERSYSYTDGSTVSGTAFYRVVEFDLDGSPTYSTTASANCGMGDSDAH
                     FYPNPVRDMLWIALNTPGASVMKIEIVDAGGARLQTQIAALSAGNNKIGIDMKKLPSG
                     LYFLNLQWENGNRTRSMTIEKD"
     gene            complement(2803..4242)
                     /locus_tag="VKQ52_20110"
     CDS             complement(2803..4242)
                     /locus_tag="VKQ52_20110"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HLZ89574.1"
                     /translation="MNKIFRLLGALMVSGITQSATAQNVGIGTTAPSDKLTVQTGSFN
                     FGISHTDGDVTVGTYIGNGYGWLGTKSFHPLAFYTGGGAAQLTIQTNGNVGIGNIIPV
                     NHLQIGSTPAFSGNDLAIGNGTQGMSFTQTSAASTWYSNVNFALMPNGGNGYVGIGTQ
                     SPANQLQIGSVGATGYGGNHIAFGNGAQASGITQTASAAQWYSTTDIVLMPRGNGHGR
                     VGINTASPGYPLAVDDYVDAPGPGYAYFTNDNQGIHLNPATPMISIAASKDVIAQGFD
                     AVSDARIKDIVGLSDGTEDLETLGKIQVTNYTLKDKINNGNHLYKKVIAQQVETVYPQ
                     VVSTHVDFIPNVYRAAGRITKTDKGWLLSFDNAHHISKGAKMLKVLASGDHTMNYCGI
                     LAIPSENEVLIDAAQLNGDKVFVYGEEVDDFRAVDYEGLTTLNISATQELEKIVKRQQ
                     AAIRAQNKKIAALSEKLEALTWTMKNRKQ"
     gene            complement(4265..>5212)
                     /locus_tag="VKQ52_20115"
     CDS             complement(4265..>5212)
                     /locus_tag="VKQ52_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89575.1"
                     /translation="VTVTTADGTATGPVFTYIAVTDVYVVGTSIYGRSYWKNGVRTDL
                     PADCGAVRGIFVSGADVYVAGADNANTPKYWKNGVGISLPMTPGNNGGIASSIFVSGM
                     DIYVAGWDMNNGAYSVPKCWKNGVALPMTFSVLGVAHSVYVDGSDVYVAGSQGPANGN
                     FIATYWKNGTPTTLTDGTSVAEATGVVVAGGSSYVSGYIEGAVQRVYWKNGIPVPLAT
                     PNTFDCSQWGIYVSAAGDVYVAGVLQNLAKYWKNGVMYDLTNTVAGPTVYESAMAVAG
                     SGTDVYIAGNGVGLGVGYWKNGVFVSLPGADQVNGIFVK"
BASE COUNT         1199 a         1389 c         1359 g         1265 t
ORIGIN      
        1 ataatctccc ttataacggc ttactatcgt gtcaaaggct cgctgaggga gaaaggcgca
       61 tacctgcgaa aagacatatt tcccgccatt catataaact tactgatttt cagaaagtta
      121 tgaaattggc gatttcaaat cgtcacgcga ctaaattctc ctgaaattca ggcgtatcaa
      181 gactttcaaa gaactgctat ttttcttaac gagacactaa tgaattcatt ttatgaataa
      241 tagcaacgaa gaacagatac gcgaagccct gaatgcgcac tggcacgcgt cggcaattgg
      301 cgatgcaaac gcggaacatg ctatttacca tgacgatgcc atctgcgact atccccagtc
      361 aggtgagcgg atcctcggac gaagcaattt gcaggccttg cggagtcatc atcccggtaa
      421 gccttcaggt ttcagcgtca ggcgaattgt cggaaaaggc gatctctgga tcaccgaata
      481 cacgatcacc taccaggggc ggccagcata cactgtgagc attatggagt ttcgcgacgg
      541 caaggtcgca cacgagactc agtatttcgc agaccctttc gaggcgccgg cctggcgaag
      601 tcaatgggtt cagcggatgc cgtagcgcaa gcggctgaac tcgccttgtc gcaaccacac
      661 cctaacaatg ccggattctg cccttttatt caagcggagc gatctttatc tttataaaac
      721 ccttaaaaag gagcggaaaa tcaaaaaaca aaagcaatca aatgagaaac ctgatcttcg
      781 caatcaacac gaccttagat ggctgctgcg accataccca attcaatccc gatgaagaaa
      841 tgatggacta ttttacgcgc ctcacgcgag atgctgacac attcctctac gggcgtaaaa
      901 cgtatcaatt gatggttcct tactggcccg atatagcgaa agaccattcc gggcagacaa
      961 aagcagatat cgaatttgcc caggcatttg acgccgtcga taaaattatt gttttctcac
     1021 aaacattaga cagccctgaa ggaaagaaca cgagaattgt tcatacgatc cttcccgacg
     1081 aaatacgtaa attgaaacaa gaacaaggca aaaatatttt aaccggtggc gtagccatcc
     1141 ctacacaact tgccgggctt ggtctgatcg atgaatatcg ttttgtggtt cacccgatcg
     1201 ttgcaggaga agggagacgg ttgtttgaag gtatcagcct gcaagagaaa ttacgattaa
     1261 aacttgttga ctcaacggtt tttaagtcgg ggtgtgttgc gcttcggtac ttgaaacagt
     1321 gaaaaaaaat gggccacaga agcgataaaa cattaatcct tttcgatcgt catgcttctt
     1381 gtcctattcc cattttccca ttgaaggttt agaaaataca gaccggaggg caattttttc
     1441 atgtcgatgc cgatcttgtt gtttcccgcg gataaggcgg ctatttgcgt ctggagcctt
     1501 gcacccccgg catcaactat ttctatcttc atgacggatg ccccgggcgt attcagcgca
     1561 atccaaagca tgtctcttac agggttgggg taaaaatggg catcgctatc acccatccca
     1621 caattggcag aagcggtggt actataggtc ggcgagccgt cgagatcaaa ctccaccacg
     1681 cggtagaaag ctgtcccgga aacggtgctg ccgtccgtgt aagagtagct cctttccccg
     1741 ttgctgtttc cggcggcggc aagatgcccg atggcctgcc attggatgcc atccatgctt
     1801 ttttccacat caaaccggct gctgttctgc tcctgcgccg ttttccagtt tatacgagcc
     1861 aatccggtga tacattcgac tataaaggaa gtgagccgga ccggcagggg attgttcaca
     1921 gttgataagg tccatctcga aaagtcggca atacccgatg cctccacata attggccgtg
     1981 gcatccctgg cggtatagcc tatattggtc cagtgaagcg tatcggtact tttccatagc
     2041 accagggcgc tctcatccag gccgttcagt tcggcatcca ggtaattgat gcgcagggtg
     2101 gcattcaatg cggtgttatt agcaggcagg atatcataat aacgaaggat ggagttcccc
     2161 actccgccgg agctggtctg cgaagcatgc ccgcgccgga tgatcgtgct tcccaggtcc
     2221 tggctggagg tgatccaggc ccccaggtta ccgggattaa agatgacggg ggcgttcagc
     2281 ctactggtag tttctacata cccgccgttg tcgctcttta tccggctgga ctcgttctcg
     2341 ccgttgagca tggccccggg ttgtaggaaa atatggttgc cattgaggtc aatcagtccg
     2401 gaattgaact cgatgccgcc ggcaatggtt atgtcttgcg ataagatgat ccctgccacg
     2461 cctgttttcg ctatttccag tttatccata agagggctgc tgctgccgga aatgctatta
     2521 tcgccggaac ctgtaaagac ccaaaacccg tcgccggcct gctggctgat gctgccgtcg
     2581 atgatcaggt cgatgtcctg gagggtgatg acggcatgcc cattggtggt tatgacggcg
     2641 ccgttttgga tggtgaactg ggcctgcgcc atggcggtgg ggacgatcag cagggccagg
     2701 accagcctgc tataccggat ggataccagc cgcccaggcc ggatgaacag atgccggatg
     2761 agtagataca taatgcttcg tttttttgcg tgtggcgttg gtttattgct tccggttttt
     2821 catggtccag gtcagcgctt ccagcttttc gctgagggca gctatttttt tattctgcgc
     2881 ccggatggcc gcctgctgcc gcttgactat cttctccaat tcctgggtag cggaaatatt
     2941 cagcgtagtg agcccttcgt aatccactgc ccggaaatcg tccacctctt ctccatacac
     3001 gaagaccttg tcgccattca attgggcggc atcgatcaac acctcgtttt ccgatgggat
     3061 ggcgaggatc ccacaatagt tcatggtatg atcgcccgaa gccagtactt tcagcatctt
     3121 cgcacctttg ctgatgtggt gagcgttgtc gaaagacagc agccagccct tatctgtttt
     3181 ggtgatcctg ccggccgccc ggtatacatt gggaataaaa tccacatggg tggaaaccac
     3241 ttgcgggtag accgtttcca cctgctgggc gatgactttt ttatagaggt gattgccgtt
     3301 gttgatcttg tctttcaggg tatagttggt gacctggatc ttaccaaggg tctccaggtc
     3361 ctctgtccca tcgctgagac ccacgatgtc ctttattctt gcgtcggaga cggcatcgaa
     3421 tccctgggca atgacatctt tactggccgc gatcgagatc atcggtgtgg caggattgag
     3481 atgaataccc tgattgtcat tggtaaaata ggcgtacccg ggaccaggtg catcgacata
     3541 atcgtccacg gcgagcggat agccgggact ggccgtattg atgcctaccc ggccatggcc
     3601 gttacccctc ggcatcagca cgatatcggt ggtggaatac cattgagccg ccgaggcggt
     3661 ctgggtgatc ccggaagcct gggcgccatt gccaaaagcg atatggttgc cgccataccc
     3721 cgtagcgccc acgctgccga tctgcagctg gttggcgggt gactgggtgc cgatgccgac
     3781 atagccgttg ccgccattgg gcatcagggc aaaattgaca ttggaatacc aggtggaggc
     3841 tgcgctggtc tgggtgaagg acatgccctg ggtaccgttg ccgatggcca ggtcgttgcc
     3901 actgaatgcc ggcgtgctgc cgatctgcaa atggttgacc ggaattatat tgccgatacc
     3961 cacattcccg ttggtctgga tcgtcagctg ggcggcgccg ccaccggtat aaaacgccag
     4021 gggatgaaaa ctcttggtcc ccagccagcc gtagccattg ccgatatagg ttccgacggt
     4081 gacgtcgccg tcggtatggg agatgccgaa gttgaaactg ccggtctgca ccgtcagttt
     4141 atcgctgggc gcggtggtgc cgatgcccac gttttgtgca gttgctgatt gggtgatgcc
     4201 ggaaaccatc agtgctccca acaggcgaaa tatcttgttc atattgattg attatgcgat
     4261 gagtttattt tacaaaaatg ccgtttacct gatcggcccc tggcagtgac acaaacacgc
     4321 cgttcttcca ataacccact ccgaggccaa ccccattacc ggctatatag acatccgttc
     4381 cgctgcccgc tacggccatg gcggattcat aaaccgtcgg tccggccaca gtattggtca
     4441 gatcgtacat gaccccattt ttccagtatt tggccaggtt ctgcaatacg cccgctacat
     4501 acacgtcacc ggcggctgag acgtaaatac cccattgaga gcaatcaaat gtattggggg
     4561 tagccagggg cacgggaatg ccattcttcc aatagacgcg ttgcaccgca ccctctatat
     4621 acccggatac atagctcgaa ccacccgcga cgactactcc cgtggcttcg gcgacgctgg
     4681 ttccgtccgt cagcgttgtc ggtgttccgt tcttccaata ggtggcgatg aaattcccgt
     4741 ttgccggtcc ctgcgaacct gccacgtaga catcgctgcc gtcgacatac acggagtgcg
     4801 ccacccccag cacgctgaag gtcattggca gcgccacccc attcttccag catttgggga
     4861 cggaataagc accgttgttc atatcccagc cggctacgta tatatccata cccgagacaa
     4921 agatggagct ggcgataccg ccattattgc ccggcgtcat gggcaggctg atgccaacgc
     4981 cgttcttcca gtatttgggc gtattggcat tatccgctcc cgcgacgtat acatcggcgc
     5041 cggagacaaa gatgcctctt acggcgccac aatcggcagg cagatcggtt cgtaccccat
     5101 tcttccaata agatcgccca taaatggatg tccccaccac atagacatcc gtcaccgcta
     5161 tataggtaaa gacggggccg gtggctgtac cgtcagccgt ggtgaccgtt ac
//
LOCUS       DATGFG010001058         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_858523, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001058 DATGFG010000000
VERSION     DATGFG010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858523"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1377)
                     /locus_tag="VKQ52_20120"
     CDS             complement(22..1377)
                     /locus_tag="VKQ52_20120"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HLZ89576.1"
                     /translation="MTIISRFRIWGKRTFDRIRNEKLKHNLLQAIPFWVASLLTGLMA
                     VLYTRLFALAERGTGYLLHRGLWVFFLVTPVCFLVAWWLVRRFAPYARGSGIPQVMAS
                     IELATPKYNEKVNRLLSLRILFVKIVSSLIMAFGGGVIGREGPTIQVAGSIFRKVNEW
                     LPAWWPKVSKRNMIMTGAAAGLAAAFNTPLGGIVFAVEELTRTHISYFKTALFTAVII
                     AGLTAQAMLGPYLYLGYPDMNGLSGTIFLGVVLVAIPAGLAGAGMSKVMLRLFAWKAG
                     FRFTRQHVLYVVGCALILAATAFFVDARVLGSGKELMTTSLFTGDKYTKWYMPLLRIG
                     GPLVSFTTGASGGVFAPALSAGASVGATISGWLQLSAANTNLLVLSGMVAFLTGVTRT
                     PFTSAILVLEMTDRHNVIFHLMLAGMISSLVAILIDRHSFYDHLKVQYLREIIHEEAA
                     Q"
     gene            1548..2972
                     /locus_tag="VKQ52_20125"
     CDS             1548..2972
                     /locus_tag="VKQ52_20125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ89577.1"
                     /translation="MKILPQKPLFFHGRVKPAGLTADWQQKWQTAGDIAVPSLERIKA
                     IGSTGSMDDYEKRKLGIFNQLNFFQLITGIIIPIAGAITNRHFPLVAWSVASFPALIS
                     VLVLVLNAQRRYDLAQIAYFVLYPIATGIVYIWGINMGVELSFILYGILSVFFIQDIS
                     QMLFALGLSMVNYFVLAVICKNYTFQLATANFYFYLFNQLLAIVFIFYGLFLIKKENT
                     EYHNSILRQKEEIAANGQLLQQQTDELTLINAFKNRLFSIIAHDLKSPIYALRNLFRN
                     MQQFDLPAEEIKGMVPEVVSELTYTTSLMENLLHWARSQMQADTVKPQMIDVSGLITE
                     VGRLLRLQAEAKHIGVTLDADASIVAFADRDMINLILRNLLSNAIKYTPEKGYIEVGA
                     RRTDTGIAIYVKDSGMGIPPDALQKIQQSNFYTTKGTAGEAGTGLGLMLCKEFVARNG
                     GALHIESLPGKGSTFTFTLPIATE"
     gene            3002..>3390
                     /locus_tag="VKQ52_20130"
     CDS             3002..>3390
                     /locus_tag="VKQ52_20130"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HLZ89578.1"
                     /translation="MKIQLWSVGRENDPYVKPGIDDFTRRITRYFPAEWNLIPTPKNA
                     ATLSQPDLKKKEAAIILGQLTKDDYLVALDEKGKQFTSENLAQFIQTRANSSNKNLVF
                     LIGGVYGLDETVLTRADHRWSLSQLTLP"
BASE COUNT          852 a         1168 c          738 g          632 t
ORIGIN      
        1 tctctacctg aaaaaatgat cctactgcgc cgcctcctcg tggatgatct cccggagata
       61 ttgcaccttg agatgatcat aaaaggaatg ccggtcgata agtatcgcca caagactcga
      121 gatcatcccg gccagcatga ggtgaaagat gacattgtgc cggtcggtca tctccagcac
      181 gagaatggcc gaagtaaacg gcgtacgcgt cacccccgtc aggaacgcaa ccatgcccga
      241 taacaccagc agattcgtat tcgcagccga cagctgcagc cacccggaga tggtggcccc
      301 cacactcgcg cccgcgctga gcgcgggcgc gaacaccccg cccgaagccc ccgtggtaaa
      361 agaaaccagc ggccccccga tacgcagcaa cggcatatac catttcgtat acttatcacc
      421 ggtaaaaagc gaagtcgtca tcagctcctt ccccgaaccc aacacccgcg catctacaaa
      481 aaaagccgtc gccgccagga taagggcaca gcccaccaca tacagcacat gctgccgcgt
      541 aaaccggaac ccggccttcc acgcaaacaa ccggagcatc accttggaca tccccgcccc
      601 ggcaagaccg gcggggatcg ccaccaacac caccccgaga aagatcgtcc cggaaagccc
      661 gttcatatcg ggataaccca ggtagagata gggcccaagc atggcctgcg ccgtcaaacc
      721 cgcaatgatc accgccgtga acaacgccgt cttgaagtaa ctgatatgcg tccgcgtcag
      781 ctcctccacc gcaaacacga tccccccaag cggtgtatta aaagccgcgg caagcccggc
      841 cgccgccccc gtcatgatca tattgcgctt cgagaccttc ggccaccagg ccggcagcca
      901 ctcattcacc ttccggaaaa tagacccggc aacctggatc gtaggcccct cccggccgat
      961 caccccaccc ccaaacgcca tgataagact cgacacgatc ttgacaaaca ggatacgcag
     1021 actcaaaagc cggttcacct tctcattgta cttcggcgtg gcaagctcga tcgaagccat
     1081 cacctggggg atcccactcc cccgcgcata gggcgcaaac cgccggacca gccaccaggc
     1141 gacgagaaaa caaaccggcg taacaagaaa gaaaacccag agcccccggt gaagcaggta
     1201 ccccgtcccc ctttccgcaa gggcaaaaag ccgggtatac aggacggcca tcaaccccgt
     1261 cagtaggctc gccacccaga acgggatggc ttgcagcagg ttgtgcttta atttctcatt
     1321 gcggatccga tcgaacgtcc tcttccccca aatacgaaac ctgctgataa tggtcataca
     1381 atattgcgtt atgataaacg ttgcaaagat agttccctca ccggctccgt cccaggttct
     1441 aataggattc taatccctgc cctttttttt aaacaaccat cttgcctttc tcgatataaa
     1501 tacctacttt cgacaggcgc ttaccgactc taacacattc aacctgaatg aaaatcctac
     1561 cgcaaaaacc attgttcttt cacggcaggg tcaaacccgc aggcctgacc gctgactggc
     1621 agcaaaaatg gcaaaccgcc ggggacatcg cagttccttc cctcgaacgc ataaaagcca
     1681 tcgggtccac cggctccatg gacgattacg aaaaaagaaa actcggcatc ttcaaccagc
     1741 tgaatttttt ccagctgatc accggtatca tcatccccat cgccggcgcc atcaccaatc
     1801 gccattttcc cctcgtcgcc tggagcgttg cctccttccc ggccctgatc agcgtgctgg
     1861 tgctcgtcct caacgcccaa cgtcgctacg atctggccca gatcgcctat ttcgttctct
     1921 atcccatcgc caccggcatc gtctatatct ggggcatcaa tatgggcgtg gaactgtcct
     1981 tcatcctcta tggtatcctc tccgttttct ttatccagga catcagccag atgctcttcg
     2041 ccctcggact ctcgatggtc aactatttcg tcctggccgt catctgcaag aactatactt
     2101 tccagctggc aaccgccaac ttctacttct atctcttcaa ccagctgctc gccatcgtct
     2161 ttatctttta cggtctcttc cttataaaga aagagaacac cgaataccat aacagcatcc
     2221 tccggcaaaa agaagagatc gccgccaacg gccagctcct gcagcaacag acagatgaac
     2281 tgaccctgat caacgccttt aagaaccgcc tcttctccat catcgcccac gatctcaaat
     2341 ctcctatcta cgccctccgc aacctcttcc gcaacatgca gcaattcgac ctgccggccg
     2401 aagagataaa aggaatggtg cccgaggtcg tcagcgaact cacctacacc accagcctga
     2461 tggagaacct gctccactgg gcccgcagcc agatgcaggc cgacaccgtc aaaccacaga
     2521 tgatcgacgt ctcgggcctg ataaccgagg tcggccgcct cctccgcctc caggccgaag
     2581 ccaaacacat cggcgtcacc ctcgatgccg acgcctcgat agtcgccttc gccgacaggg
     2641 atatgatcaa cctgatcctc cgcaacctcc tctccaacgc catcaagtac acgcccgaga
     2701 agggttatat cgaagtcggc gcccgccgca ccgacaccgg tatagccatc tacgtaaaag
     2761 attcgggcat gggtatccca cccgacgccc tgcaaaagat ccagcaaagc aatttctata
     2821 cgacaaaagg gacggccggc gaagccggta ccggcctcgg cctgatgctc tgcaaggaat
     2881 tcgtcgcccg caacggcggc gccctccata tcgagagcct gcccggtaaa ggcagcacct
     2941 tcaccttcac actccccatc gccacggaat gatcgccatg gaataatcct acctttgccg
     3001 gatgaaaatt cagctttggt ccgttggcag ggagaatgac ccctacgtca aacccggcat
     3061 cgacgacttc acccggcgta tcacccgcta cttccccgcc gaatggaatc ttatccccac
     3121 accaaagaat gccgccaccc tctcccagcc cgatctgaaa aaaaaagagg ccgccatcat
     3181 cctcggtcag ctgacaaagg atgactacct cgtggccctc gacgaaaaag gaaaacaatt
     3241 cacctccgaa aaccttgcgc aattcatcca gacccgggcc aacagcagca ataagaacct
     3301 tgtctttctc atcggcggcg tttatggcct cgatgagacc gtcctcaccc gggccgacca
     3361 ccgctggtcc ctctcacaac tgaccctgcc
//
LOCUS       DATGFG010001059         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_586060, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001059 DATGFG010000000
VERSION     DATGFG010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586060"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1043)
                     /locus_tag="VKQ52_20135"
     CDS             complement(204..1043)
                     /locus_tag="VKQ52_20135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ89579.1"
                     /translation="MSTNNNTAAAGEQVKAIIIDDEPLARMIVQEYLQSYSEIVVLQE
                     CGDGFEGIKAIQQHHPDLIFLDIQMPKINGFEMLELVEEPPAVIFTTAFDEYAIKAFE
                     SHAVDYLLKPFNKERFDKALRKWLDQRVTRTSDRITHAPGAIAHGPAGAAPNAADTEA
                     LLETAAQSPAQQQRIVVKTGGKIKIIPLEDIHFLEAADDYVKIHTHNGAFLKNRTMSY
                     FEKTLDPAQFVRTHRSYILNVQQVTRIDPYEKDSHLCILQSGATVPVSKAGYVKLKGV
                     LGL"
     gene            complement(1040..2125)
                     /locus_tag="VKQ52_20140"
     CDS             complement(1040..2125)
                     /locus_tag="VKQ52_20140"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ89580.1"
                     /translation="MAKSPLSTYKFLLLFISWWAIWAFLQVSLLIGFGLPTAIALSDS
                     IVSNTFVALSCGFLSNNMQYYLPKKERYWYILFISLGLSGIILLACKAILVPLLSDRA
                     DGAGYAQFFSKSWAVRFDIVFLQVACMAILSVLWYSSAEEQENRQRRNDAERLAKEAE
                     LFKLRQQLAPHFLFNSLNSISALIGSRPEQARKMIQQLSDFLRGNLKKEEQQWVPLSE
                     ELQYLELYLDIEKVRFGHRLDTQIVCAEDCMGSKLPPMLLQPIVENAIKFGLYDTTEA
                     VTISIRARIEAKFVIIEVENPFDPETSRPKKGTGFGLSSIQRRLYLLFARADLLETRV
                     AGNLFTTIVRIPQTDNQAPAIENKQTT"
     gene            complement(2250..>2633)
                     /locus_tag="VKQ52_20145"
     CDS             complement(2250..>2633)
                     /locus_tag="VKQ52_20145"
                     /inference="COORDINATES: protein motif:HMM:NF025484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HLZ89581.1"
                     /translation="ANAYKLKLMNWYLSKIIFRIICGEGDHTPQFDEQLRLIAARDEE
                     EAFGKAVAIGRREQDAFYNHEQQLVQWKFVNVAELYRFSGLLDGAEMYSRIQETDDPD
                     AYIDQANRRAEHIQACTTHKMLRLL"
BASE COUNT          464 a          624 c          915 g          630 t
ORIGIN      
        1 ccttttgtgt gcaggtcgtt gagtgcggcc tggagggagg ggtagctgcc ggtggggcct
       61 actgagagcg atccgccaag tcctaaggga caggaggtct gggaacggac ggaaaggaca
      121 gtgaaggcca ggaaaagggc caggagagca gttcgtttca tggcggttgg ggttacccta
      181 aaggtacggt ccgccccgtg aacttacaac cccagtaccc cctttaattt cacatagccc
      241 gccttgctca ccggaacggt ggcgccggat tggaggatgc agagatggct gtccttctcg
      301 tagggatcga tgcgggtcac ctgctggacg ttgaggatat aactccggtg tgtccggacg
      361 aactgcgcgg ggtcgagggt tttctcgaag tagctcattg tccggttctt aaggaaggcg
      421 ccgttgtgcg tatggatctt gacgtagtca tcggcggctt cgaggaagtg gatgtcctcg
      481 agggggatga tcttgatctt gccgcccgtt tttacaacga tgcgttgttg ctgggccggg
      541 gactgggccg cggtttcgag gagggcctcg gtgtctgccg cgtttggcgc cgcgccggcg
      601 ggaccatggg cgatcgcacc cggggcgtgg gtgatgcggt cggatgttcg ggtcacgcgc
      661 tggtcgagcc atttgcggag ggccttgtcg aagcgttcct tattaaaggg ttttaacaaa
      721 tagtccacgg catggctctc gaaggccttg atggcgtatt cgtcgaaggc cgtggtaaag
      781 atgacggcgg ggggctcctc gaccagttcg agcatctcga agccgttgat cttgggcatc
      841 tgtatatcga ggaagatgag atcggggtgg tgttgctgga tggccttgat cccttcgaag
      901 ccgtcgccgc attcctgcag tacgacgatc tccgaatagg actgcagata ttcctgtacg
      961 atcatccggg cgagcggttc gtcgtcgatg atgatggctt ttacctgttc gccggctgcg
     1021 gcagtgttgt tgttggtgct catgttgttt gcttattttc gatggcgggg gcctggttgt
     1081 cggtttgcgg gatacggacg atggtggtga agagattgcc cgcgacgcgt gtctcgagaa
     1141 gatcggcgcg agcgaagagg aggtacagtc tacgctgtat tgaactcagc ccgaagccgg
     1201 tacctttttt cggcctcgat gtctccgggt cgaaggggtt ctccacctcg atgatcacga
     1261 attttgcttc gatgcgtgcg cgtatcgaga tagtgacggc ttctgtcgta tcatagagtc
     1321 cgaattttat ggcgttctcg acgattggct gcaggagcat cggcggcagc ttgctgccca
     1381 tgcagtcttc ggcgcagacg atctgcgtat cgaggcggtg gccgaagcgt accttttcga
     1441 tgtcgagata gagttcgagg tattgcagct cttcggagag cggcacccat tgttgttctt
     1501 cttttttcag gttgccgcgg aggaagtcgg agagctgctg gatcatttta cgcgcctgtt
     1561 cggggcggct gccgatgagg gcgctgatgg agttgaggct gttgaagaga aaatgcggcg
     1621 cgagctgctg gcgtagcttg aagagttcgg cttccttcgc caggcgttcg gcgtcgttgc
     1681 ggcgctggcg gttctcctgt tcttccgcgg aggaatacca gagaacgctg agtatcgcca
     1741 tacaggccac ctgtaaaaag acgatgtcga agcggaccgc ccagctcttg ctgaagaact
     1801 gcgcatagcc ggcgccgtcg gccctgtcgc tcagcagggg cacgaggatg gccttgcagg
     1861 cgaggaggat gatgccgctg aggccgaggc tgataaagag gatataccag tagcgttctt
     1921 ttttcggcag gtagtattgc atgttgttgc tgaggaagcc gcagctcagc gcgacgaagg
     1981 tgttggacac gatgctgtcg cttagggcga tggcggtcgg gaggccgaag ccgatcagca
     2041 gcgagacttg caggaaggcc cagatggccc accaggagat gaagaggagg aggaatttat
     2101 atgttgaaag cggtgatttg gccatggttt ttgccggtgc tttggttgct tgcgggtgca
     2161 gaggtagttt gggtcgccgg cgggtggtcc cggacaatgg gtcggttgcg ggttgcggct
     2221 tacaggactg ctgcctgcag gactggggct tacaggaggc gtaacatctt gtgggtcgtg
     2281 caggcctgga tatgttcggc gcggcggttg gcctggtcga tatacgcgtc ggggtcgtcg
     2341 gtctcctgga tgcgggagta catctcggcg ccgtcgagga gtccgctaaa ccggtagagc
     2401 tctgcgacat tgacgaattt ccactggacg agctgctgtt cgtggttgta gaaggcatcc
     2461 tgttcgcgcc ggccgatggc tacggccttg ccaaaggcct cctcttcgtc acgggccgct
     2521 atgaggcgca gctgttcgtc gaattgcggg gtgtggtcgc cttcgccaca gatgatccgg
     2581 aagatgatct tgctgagata ccagttcata agcttgagtt tatatgcgtt ggc
//
LOCUS       DATGFG010001060         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_861395, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001060 DATGFG010000000
VERSION     DATGFG010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861395"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VKQ52_20150"
     CDS             <1..240
                     /locus_tag="VKQ52_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89582.1"
                     /translation="PDAAVTSLDGKVYSKRIPRDTIDQYRAVYPIISTQLKAGGLHIR
                     ILADQNPGNGFQPSPPNLEDVYFSHIATKMDVLTI"
     gene            293..>2984
                     /locus_tag="VKQ52_20155"
     CDS             293..>2984
                     /locus_tag="VKQ52_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89583.1"
                     /translation="MFSQIFLFEIRYRLRRPAFYVYFAVVLGFGFLAFFQGNLPAVEK
                     EMINSPAILAEFCAAASIFLLLVSSAIMGTPLYRDLEHNTKEYYLSYPITKAGYFWGR
                     FLGSFSFVLVISLAVILGAWLGCLLGPVLGWQSPHRYGPNRLMYYLYPWLTFVVPTLF
                     FTSSLFFGLVAIFRNVKVIYSSGLFLFLGYILANFFLHNIHNDTVIYLADPFLQNGLR
                     IAVAGLSPEQLNTHIIPLTGLVLYNRLLWVSIGALVLAGTWWRFSFERFFSGRAAKDK
                     RDNRPAPSIPVGPGAIHTQLQGPYYRRTLLSLTRIEILNIIRDNYFWIILSGGYIFLS
                     FVFSHGPGRYDVHDYPRTVFFVDIFGDIFLFFIFLIIVFYTGETVHREKLTRFAFIND
                     ALPPPDWILVTAKLLSLCALAVFLSLTPFLIGFPIQLLKGYTAFNIPQYLSSTFVDIL
                     PRLITMVFFCYAVHLIVNNKFAAHGIAITIWVVMFILVTFNYFTYFLLLYSYTPQEWA
                     SDMDGIGHMVRSILWYQGYWTVAGLLLLLIGGLFFARGVPRSPGERLRLARQRFHGPA
                     RIGSWALFAVFLTLGAYIYYNVSYLNEFTTPWERNERKALVEKELKRYASLPLPRITS
                     MDMTVDLFPDKQREETNAMVTVMNKGNLPIDSLLIDGDGLDFELKYNGAVLPYTCPLY
                     FATGKFSLFRPRKEASDYRLYILPLVLRPGDSMQVQIHSVLSFRGFQNNLYAPNALHN
                     GMFTTGNLPGLGYDEGDEIGNNDIRKAHGLPEKKDVDIPWNDSAGRNSLFDGVNADLV
                     NYNLTVSTSADQWVQSSGRLEREWTEGNRNYFHFVQDHPGLYPPVAMVSARYNKWSDT
                     VQLDNGRTVQVEIDYHPVHSNNLRHYQAALKDGLRYFSRAF"
BASE COUNT          601 a         1050 c          644 g          689 t
ORIGIN      
        1 cccgacgccg ccgtgacatc cctggatggt aaggtctata gcaagcgcat cccccgggac
       61 accatcgacc aataccgggc cgtctacccg atcatctcca cccagctgaa agcaggcggg
      121 ctccatatcc gcatcctcgc cgaccaaaac cccggcaacg gcttccagcc cagccctccg
      181 aacctggaag acgtctactt cagccatatc gccacaaaaa tggacgtatt gaccatctga
      241 caaaccatca tactataacg acctggtata acctaccata aaacctgcaa ctatgttctc
      301 ccagatcttc ctcttcgaga tccggtaccg gctgcgccga ccggccttct atgtctattt
      361 cgccgttgtc ctcggctttg gcttcctcgc cttcttccag ggcaacctcc ccgccgtgga
      421 aaaggaaatg atcaactccc cggccatact cgccgagttc tgcgccgccg ccagcatctt
      481 cctcctgctg gtgagctcgg ccatcatggg cacccccctt tatcgcgacc tcgaacacaa
      541 cacaaaggaa tactatctct cctaccccat cacgaaggcc ggctatttct ggggccgctt
      601 cctcggctcc ttctccttcg tcctggtgat cagcctcgcc gtgatcctcg gcgcctggct
      661 cggttgcctg ctcgggcccg tcctcggctg gcagtccccc caccgctacg gccccaaccg
      721 ccttatgtac tatctgtacc cctggcttac cttcgtcgtc cctacccttt tctttacttc
      781 gtccttgttc ttcgggctcg tcgccatctt ccgcaacgta aaagtcattt acagcagcgg
      841 cctctttctc ttcctcgggt atatcctggc caatttcttc ctccacaata tccataacga
      901 cacggtcatc tacctggccg atccctttct ccagaacggc ctgcgcatcg ccgttgccgg
      961 cctttccccc gaacagctga acacccatat cattccccta accgggctgg tcctctacaa
     1021 tcgcctcctc tgggtctcga ttggcgccct cgtactcgcc ggcacctggt ggcgcttcag
     1081 cttcgaacgt ttcttcagcg gccgcgcagc aaaagacaaa cgcgacaacc ggcccgcccc
     1141 cagcatcccc gtcggccccg gcgccatcca tacccaatta cagggtcctt attaccgccg
     1201 caccctcctc agcctcaccc gcatcgagat cctcaacatc atccgggata attatttctg
     1261 gatcatcctc tccggcggct atatcttcct gagcttcgtt ttctcgcacg gcccgggtcg
     1321 ctatgacgtc cacgactatc ccagaacggt gttcttcgtc gatatcttcg gggatatctt
     1381 cctcttcttt atcttcctga tcatcgtatt ctacaccggc gagaccgtcc atcgcgaaaa
     1441 actgacccgc tttgccttta tcaacgacgc cctgccccca cccgactgga tcctcgtcac
     1501 cgcaaaattg ctcagtctct gcgccctggc cgttttcctc tcgctgaccc ccttcctcat
     1561 cggcttcccc atccaactgc tgaaagggta tacggcattc aatatcccgc aatatctctc
     1621 ttccaccttc gtcgatatcc tccccaggct gatcaccatg gtcttcttct gctatgcggt
     1681 ccacctcatc gtcaataaca agttcgccgc ccatggcatc gccatcacga tctgggtcgt
     1741 catgttcata ctcgtcacct tcaattactt cacctatttc ctgctgctct attcctatac
     1801 tccccaggag tgggcctccg atatggacgg catcggacac atggtaagat ccattctctg
     1861 gtaccagggg tactggacgg tggccggcct gctgctgttg ctcatcggcg gcctcttctt
     1921 tgcccgcggc gtgccccgct ccccgggcga acggctgcgc ctcgcccggc aacgcttcca
     1981 tggccccgca cggatcggct cctgggccct gttcgccgtc ttcctcaccc tcggcgccta
     2041 tatttattat aatgtcagct atctcaacga attcaccacc ccctgggaaa ggaacgaaag
     2101 aaaggccctg gtggaaaaag agctcaagcg gtacgccagc cttccccttc cccggatcac
     2161 cagcatggat atgaccgtcg atctcttccc cgataaacaa cgggaagaaa caaatgcaat
     2221 ggtaaccgtc atgaacaagg gaaacctgcc gatcgacagc ctccttatcg acggcgacgg
     2281 cctcgatttc gaactaaaat acaatggcgc ggtccttccc tacacctgcc ccctctattt
     2341 cgccaccggc aaattcagcc tcttccgccc ccgcaaagag gcttccgact accgcctcta
     2401 tatcctgccc ctcgtcctga ggccgggaga ctcgatgcag gtgcaaatac actcggtgtt
     2461 atccttccgc ggttttcaaa ataatctcta tgcccccaat gccctgcaca acgggatgtt
     2521 cactaccggt aacctgcccg gtcttggcta tgatgaggga gatgagatcg gtaacaacga
     2581 catccggaaa gcccatggtc tgccggaaaa gaaagatgtc gacatcccct ggaacgactc
     2641 cgccggcaga aatagtttgt tcgacggcgt caacgccgat ctggtgaact ataacctcac
     2701 tgtcagcacc agcgccgacc aatgggtaca gtcctccggc cgcctggaaa gggaatggac
     2761 cgaaggcaac cggaattact ttcattttgt ccaggaccat cccgggctct atccaccggt
     2821 agccatggtc tcggcacgat ataataaatg gagcgatacc gtacaactcg acaacggtcg
     2881 caccgtacag gtggagatcg attatcatcc ggtccattca aacaacctgc gtcattacca
     2941 ggcggcgcta aaggatggcc tgcgttattt ctcccgggca ttcg
//
LOCUS       DATGFG010001061         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_758512, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001061 DATGFG010000000
VERSION     DATGFG010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758512"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VKQ52_20160"
     CDS             complement(<1..213)
                     /locus_tag="VKQ52_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89584.1"
                     /translation="MDQKKIRVLLLFRQPGRFFSLERIFRQLTPILDEEVTVSRWEAP
                     YSRASLRDIWNNLRSARQCRADVYHVT"
     gene            complement(221..1627)
                     /locus_tag="VKQ52_20165"
     CDS             complement(221..1627)
                     /locus_tag="VKQ52_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89585.1"
                     /translation="MNITSRYKIQFVGITNVVIMGALVLVAFLSNGIYLLACLATIYL
                     VMMLLWRSHRPGILVFAFVMQWTQIAAYVVWMNVMGKDINFLSPHAGIAVLMSCLGVL
                     VMALVLSQRVNSLPIPSSEQLSAQAKLINEKKMLMLYLFSTFFLGGIGFILGPSSGFA
                     QILITLSSLKWAFFLVYAFVCWINKKNRVLLIVIILFEFTTSLYSYFSSFKEVILMTI
                     LLALTFVRRISFRQFMNGFLTALVLGFLLLTWTAIKNDYRQFLNGGTRKQVVGVSRSE
                     AFSKIEEKVGTLSVEDYQNAGMLFLYRLQYIYHLAKTMDRVPEVLPYEYGAVWWGNIT
                     FVLEPRLLFPDKPIYEATVKTNKYTGIRYAGFKLGSSFSLGYFADSYIDFGFVGMVIP
                     LALIGFFVSLIYRSLYRLKGLNLLIRYALINVALFEFGSFEADGLFLFGRLLLTILVF
                     YTLAKTVLPPLQRWLYKA"
     gene            complement(1624..2364)
                     /locus_tag="VKQ52_20170"
     CDS             complement(1624..2364)
                     /locus_tag="VKQ52_20170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLZ89586.1"
                     /translation="MSLVEEKYNDWHRNYADSISREDEIKLMLWHKNVLSVLKKEELK
                     GLRVLEIGCGIGDFSIYLAKNFDVQVTGTDFSPMAIEIAQKKQSIHAGRVDFRVADAQ
                     DLPFEDASFDLIISCECIEHVPNPQKMIDEMHRVLKDKGRFVLTTENYSNAFAYYFLY
                     LKLAKKSYDSGSGEQPIENFFVYWRILAKFRRAGFKQVRCFGNEFTFLLIPGTAPDKF
                     ALKDTRSPLLRRLLKPLGRRMTYYGVKE"
     gene            complement(2370..3476)
                     /locus_tag="VKQ52_20175"
     CDS             complement(2370..3476)
                     /locus_tag="VKQ52_20175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLZ89587.1"
                     /translation="MKKRILFFAGGAYISGLEIVTLHLIRKLKSEGHELKCVINGWND
                     GMFIRELEKVGVPYEEAKLGWLYFRKPLWTLDSLVHLPKAYFKSRRIIREFDPDVIHY
                     CNYATVILLYPLLRNRPGVYNLQEPHEPNRKNLTIYRLLNKRVNVFTGVSEYIGGVLT
                     RLGIPKQKISLVYNGVPPIPDPPVKPEEEKLVFGIIGQVASWKGHETLIEAIHLLQGA
                     SRPFVVKIFGNDKNAYAEELRKKIAEKKISAYFEWEGFVREQKAIYDKVDVVVVPSLS
                     QEPCSLTIIESMMYGKRLIVSDRGGNPELVEHRRTGLIFPAEDARQLMECLRLYLEKD
                     AGTASIPAQAHDRAMHCFTDDRMTAVYTEIYSKL"
     gene            complement(3473..>4159)
                     /locus_tag="VKQ52_20180"
     CDS             complement(3473..>4159)
                     /locus_tag="VKQ52_20180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLZ89588.1"
                     /translation="KHGVSSDRLVYLPHVIDNGRFEASAAEQERKAKEWRRELGIGEQ
                     DLVVLFAGKLEPKKNPLFLPRLATAVTSPEVKFLVVGNGVMEQELKELAREDKRFFFA
                     DFQNQSTMPVVYRAGDVFILPSQGPDETWGLAINEAMACSRPVLVSTRCGCAPDLVRE
                     EQNGIIFESHDITRCAKFLNSMVKDRASLKNMGNRSLQLIQEFTFHRITTTLLEFIGA
                     RLTLTPAKQK"
BASE COUNT          981 a         1193 c         1074 g          911 t
ORIGIN      
        1 cgtgacatga tagacatccg ccctgcattg gcgcgccgaa cgcaaattat tccagatatc
       61 ccgcaacgat gcccgggaat acggagcctc ccatcggctt accgtcacct cttcgtcgag
      121 aatgggcgtc aattgccgga agatccgttc caggctgaag aagcgccccg gttgacggaa
      181 gagaaggaga accctgatct ttttttgatc catactatta tcaggctttg tagagccatc
      241 gctggagagg gggcaacacg gtcttggcca acgtataaaa aaccagtatc gtcagcagca
      301 gacggccgaa aaggaacagg ccgtccgctt cgaaggagcc gaattcgaac agggccacat
      361 tgatcagggc gtaccggatg aggagattca accccttcag ccggtagaga ctgcgataga
      421 taagactcac aaaaaagccg ataagggcca gcgggatgac catgcccaca aacccgaagt
      481 cgatgtagga gtccgcaaaa tacccaaggc taaaggatga ccccagcttg aaaccggcat
      541 accggatacc cgtgtatttg ttcgtcttca ccgtcgcctc ataaatgggt ttgtccggga
      601 agagcagccg cggctccagt acgaaagtga tattgcccca ccagacggcg ccatactcgt
      661 agggcaggac ctccggcacc cggtccatgg tcttggctaa gtgatagata tactgcagcc
      721 ggtagaggaa caacatcccg gcgttttgat agtcctccac cgaaagcgtt cccacttttt
      781 cctcgatctt gctaaacgct tccgatctcg acacccctac cacctgtttg cgggtcccgc
      841 cgtttagaaa ctgccggtaa tcgttcttga tggccgtcca ggtgagcagg agaaagccca
      901 gcaccagcgc cgtcaggaaa ccattcatga actgccggaa actgatccgc cggacaaagg
      961 tcagcgccag caggatggtc atcaggatca cctccttgaa actcgaaaaa taggagtaca
     1021 gggaagtcgt gaactcgaac aggatgatca cgatcaggag cacccggttc tttttattga
     1081 tccagcagac aaacgcatag accaggaaga aggcccattt caggctggaa agcgtgatga
     1141 ggatctgggc aaagccgctc gacggaccga ggatgaaccc gataccgccg aggaaaaagg
     1201 tggaaaagag atacaacatc agcatcttct tttcattgat cagtttcgcc tgcgcgctca
     1261 actgttcgct gctgggaatc gggagcgagt tcacccgttg ggaaagcacc agcgccatga
     1321 caagcacccc caggcaggac atgaggacgg caattcccgc atggggactc aggaaattga
     1381 tgtccttccc catcacattc atccagacca cataggctgc gatctgcgtc cattgcatca
     1441 cgaacgcaaa gaccaggata cccggccggt gactgcgcca caacaacatc atgacaaggt
     1501 agatcgtcgc cagacaggca agcaggtaga tcccattgct caggaacgca accagcacca
     1561 gggcgcccat gatcacgaca ttggtgatcc cgacgaattg tatcttgtat cgagaggtaa
     1621 tattcattct ttcactccgt aataggtcat ccggcggccc agcggtttca acagccttct
     1681 cagcagaggc gaccgggtat ctttcagcgc aaatttgtcg ggtgcggtgc ccgggatcag
     1741 caaaaaggta aactcattgc caaaacacct cacctgtttg aatcccgctc ttctgaactt
     1801 cgccaggatc cgccagtaga caaagaaatt ttcgatcggc tgctccccgc tgcccgaatc
     1861 ataggacttc ttcgccagtt tcaggtagag gaaataatag gcgaaggcat tggaatagtt
     1921 ctcggtggtc agcacgaacc ttcccttgtc tttcaggacc cggtgcatct cgtcgatcat
     1981 cttttgcggg ttcggaacat gttcgataca ctcgcaggag atgatcaggt cgaacgaagc
     2041 gtcttcaaac ggcagatcct gcgcatcggc cacccggaag tccacccttc cggcatgaat
     2101 ggattgtttc ttctgggcga tctcgatagc catcggggaa aaatccgttc ccgtcacctg
     2161 cacatcgaaa ttttttgcca ggtagatcga gaaatcgccg atcccacagc ctatttccag
     2221 cacccggagg cctttcagct cctctttctt caaaacggaa agcacgtttt tatgccagag
     2281 catgagcttg atctcatcct ccctcgaaat ggagtcggca taattgcgat gccagtcatt
     2341 gtatttttcc tcaacaaggc tcatattggc taaagtttgg aataaatttc ggtataaacc
     2401 gcggtcatcc ggtcgtcggt aaagcagtgc atcgcccggt cgtgcgcctg ggcgggaatg
     2461 gacgccgtgc cggcatcctt ttccaggtaa agccggaggc attccatcag ttgccgcgcg
     2521 tcctccgccg gaaagatcaa ccccgttcgc cggtgttcga ccagctcggg attacccccg
     2581 cggtcggata cgatcaggcg cttcccgtac atcatcgatt cgatgatcgt aagagagcag
     2641 ggttcctgcg agaggctggg tacgacgacc acatccacct tgtcatagat cgccttctgc
     2701 tcccgcacaa agccttccca ttcaaagtac gcgcttattt ttttctccgc gatctttttt
     2761 ctcagctctt cggcataggc gttcttatcg tttccgaata tcttgacaac gaagggtcgc
     2821 gacgccccct gcaagaggtg gattgcttcg atcagggtct cgtgcccctt ccaggaagcc
     2881 acctggccga taataccaaa aacaagtttc tcctcctccg gctttaccgg cgggtcgggt
     2941 atcggcggaa cgccattata gaccaggctg atcttctgct tcggaatgcc gagcctcgtc
     3001 agcacaccac cgatgtattc cgaaacaccg gtaaagacat tcacccgttt gttcaacagc
     3061 cggtagatag taagattctt gcggttgggc tcgtgcggct cctgcaggtt atatacaccg
     3121 ggcctgttcc tcaacagcgg atacaaaagg atcacggtgg catagttaca ataatggatg
     3181 acatccgggt cgaactcccg gatgatccgc cgggatttga agtacgcttt cgggagatgt
     3241 acgaggctgt ccagggtcca gaggggtttg cggaaataaa gccatcccag cttcgcctct
     3301 tcataaggca cccccacctt ctccagttcg cggatgaaca tcccgtcatt ccagccgttg
     3361 atgacacatt tcagctcatg cccttccgac ttcagcttcc tgatcaggtg aagggtgacg
     3421 atctccagtc cggagatata ggcgccgccc gcaaagaaca agatcctttt tttcattttt
     3481 gtttggccgg ggttaacgta agccgggcgc cgataaactc gagaagggtg gtggttatcc
     3541 ggtgaaaagt aaattcctgg ataagctgca ggctgcggtt gcccatattt ttcagggatg
     3601 cccggtcctt caccatgctg ttgagaaatt tggcacagcg ggtaatatca tgggattcga
     3661 agatgatccc gttctgttct tccctcacca ggtcgggcgc gcagccacac cgggtggaca
     3721 ccagcacggg ccgggaacag gccatcgcct cgtttatcgc aagaccccat gtttcatcgg
     3781 gcccctgcga tggcaggata aagacatccc cggcacggta aaccaccggc atcgtcgatt
     3841 gattttgaaa gtcggcgaaa aagaaccgct tgtcctcccg ggccagttcc ttcagctcct
     3901 gctccatgac gccgttgccg accaccagga atttcacctc cgggctggtg accgccgtcg
     3961 ccagcctcgg caggaagagg gggttctttt tcggctccag cttgccggca aacagcacga
     4021 caagatcctg ttccccgatg cccagctccc ggcgccattc ttttgccttc cgctcctgct
     4081 ccgcggccga ggcctcgaag cggccattat cgatgacatg cggcaggtag accagccggt
     4141 cgctgctgac cccatgttt
//
LOCUS       DATGFG010001062         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_628679, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001062 DATGFG010000000
VERSION     DATGFG010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628679"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3094)
                     /locus_tag="VKQ52_20185"
     CDS             complement(<1..3094)
                     /locus_tag="VKQ52_20185"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLZ89589.1"
                     /translation="MVQEGMLYHYLKDENQNLYNVQLSFDIKGHLNVPVLQDAFDRVQ
                     SANQVLRSVFRWEEISKPVQIIFHRCAIPIAYTDVSQRDGKDRYGYVEDYLRRDWHER
                     FDLTKPPLRLHVVKLADRSFVLIITHHHILYDGWSTAILLKDLFYNYNQLTGGKEPVS
                     MQRPSYKDTCMVIRRNAVAANGDVFWKDYLQGYQPTAFFSKNYAPAAGAGGLEKEQLT
                     IGDINLDGFCSTYNVTKAALVYAAYAIFLQKWQDAPDVVFGTAVSTRSAAIKGQEHVI
                     GNFINTIPLRLADGENSSFYEVVTRLNKELLTRSQFADTSYFDIKRLFRLKPSDNLFD
                     SVVAIENYPIHQALTNGNDPDIQLRSVNENTGIPLMLTVFFGNGLQLEFAYDTGMVRG
                     SFIKALSDFFSCTLKEMLDNPNGKIKGFHLVREDTRQYLLHHLNNTLADYPRTETVIS
                     LFERQVQEYPDNIAVKFANGAFTYRELKAEMDQIASWLRDIKGIRPGDLVGVMLHREQ
                     LLIPSIFGILKAGAVYVPLDVTAPAERVASLIQDARLKAVITRGRYAPPLEAIAGLVV
                     DLDKVSDEIHAHRAVPGAVTLDEHDLAYVIFTSGSTGQPKGVMIEHHSVVNRLVWMQK
                     EYPLTSYDVLLQKTPLVFDVSIWELFWWAMTGASLYLLENGGEKDPRKIISTIRENGV
                     TTVHFVPSMLTGFLSVMDDAFDFSVLRSLRFVFTSGEALTPPQVDSFGKTINRHGGSR
                     LINLYGPTEATVDVSFHECAFHGEYVSVPIGKPIDNTRLYIVSRDSQLLPPGAVGELC
                     IAGAGLARGYLNREALTAEKFVACPFSQGGRMYKTGDLARWLPDGSIEFLGRLDHQVK
                     IRGVRIEPGEIEYQLLTHPEVKEAVVVAKEYDAQRFLVAYYVGGSPLEAHRLKAHLTA
                     RLPDYMIPAYFVQLSAIPLTGNGKLDRKLLPEPALGLDTGEEPLQGISEAERQLVNVW
                     AEVLGHDKIGVNRSFFDIGGDSLKALKVVNRISRQLRIGLSIREFFDNPTIRQLSQYL
                     E"
BASE COUNT          774 a          861 c          746 g          801 t
ORIGIN      
        1 tttccaggta ttggctcaat tgcctgatag tgggattgtc gaaaaattcc cggatcgata
       61 gcccgatacg cagctgcctg gaaatacggt tcaccacctt gagggccttc aatgaatcac
      121 cgccgatatc gaaaaagctc ctgttgaccc cgatcttatc atgccccagt acttcagccc
      181 agacgttcac cagctgtcgt tccgcctcgc ttattccttg caatggctcc tctccggtat
      241 cgaggccgag cgccggttcc ggcagtaatt tgcggtccag ctttccattg cctgtgagcg
      301 gaatggcaga cagttgcacg aaataggcag ggatcatata gtcggggagc ctggccgtca
      361 gatgagcttt tagccgatgt gcctccaaag gtgacccgcc aacataatag gcaacaagaa
      421 atcgctgagc atcatattct tttgcgacca ctacggcctc tttgacctcc ggatgggtga
      481 gcagctggta ttctatctct cctggttcga tccttacacc ccttattttt acctggtggt
      541 ccagccttcc aaggaattca atgctcccgt cgggcagcca tcttgcaagg tcacccgttt
      601 tatacattcg accgccttgc gaaaatggac aagccacaaa tttttctgcg gtaagcgcct
      661 ctctgttcag gtaacctctt gccagccctg ccccggcaat acacaattca cccactgcac
      721 ccggtggcaa tagctggctg tccctgctaa caatatacaa ccgggtatta tcaatgggtt
      781 ttccaatagg gacagataca tattccccat ggaatgcgca ttcatgaaac gatacgtcca
      841 ccgtggcttc tgtggggcca tacaggttta tcaaccggct gccgccgtgc ctgttgatcg
      901 tcttgccaaa actgtccacc tgcggaggtg tcaatgcctc cccgctcgta aacacaaatc
      961 gcaggcttcg caggactgaa aagtcaaatg catcatccat cactgaaaga aatccggtta
     1021 gcatggaggg cacaaaatgg acggtggtta cgccgttctc ccgtatcgtg ctgatgattt
     1081 ttcgcggatc tttttcgccg ccgttttcca gcaggtacag ggaagcgcct gtcatcgccc
     1141 accagaacag ctcccaaatg gaaacatcga atacaagcgg cgttttctgc agcagaacat
     1201 catatgaagt aagcggatat tccttttgca tccatactaa cctgttgacc accgaatggt
     1261 gctcgatcat cactcccttc ggttgaccgg tagaccccga agtaaagatc acataagcca
     1321 ggtcatgctc atccagcgtc accgcgccgg ggactgcccg gtgggcatga atttcatccg
     1381 acactttgtc caggtcgaca accagcccgg caatagcctc gaggggaggc gcatacctcc
     1441 cccgtgttat caccgccttt agtcttgcat cctgtataag cgaagcgacc ctttccgcgg
     1501 gagcagtcac gtcgagtgga acatagacgg ccccggcttt caggatccca aaaatggaag
     1561 gtatcagcag ctgttcccga tggagcataa cccccaccag gtcacccggt ctgatgccct
     1621 ttatatccct cagccaggag gctatctgat ccatctctgc cttcaactcc ctgtaggtaa
     1681 aggccccatt ggcaaatttt accgctatgt tatcgggata ttcctgaacc tgccgctcaa
     1741 aaagagagat aacggtttca gtgcggggat aatccgccag cgtgttattc aaatgatgca
     1801 gcaggtactg ccgggtatct tcccggacaa gatgaaaccc ttttatcttt ccgttgggat
     1861 tatccagcat ttcctttagg gtacaggaaa agaaatcgct taaagccttt ataaaggaac
     1921 ccctcaccat gcccgtatcg taggcaaatt caagctgcag gccgtttcca aagaacaccg
     1981 taagcatcaa cgggatcccc gtgttctcat tgacagaccg caattgaata tccgggtcat
     2041 taccgtttgt aagtgcctgg tggatgggat aattctcgat ggcgaccacc gaatcaaata
     2101 aattgtcgga gggttttaat cggaacaggc gcttaatgtc gaagtaggag gtgtcggcaa
     2161 actggctcct cgtcaataat tctttgttca gcctcgttac aacctcataa aaggaggaat
     2221 tttccccatc cgccagccga aggggaatgg tgttgataaa attgccgatg acatgctcct
     2281 gtccctttat ggcagcagac cgggtagata cagcggtacc aaaaacaaca tccggcgcat
     2341 cctgccattt ctgcaggaag atggcatagg ccgcatacac gagcgccgcc ttcgttacgt
     2401 tgtacgtact gcaaaaccca tccaggttga tatccccgat agtcagctgt tccttttcaa
     2461 gtcccccggc accggcggca ggtgcataat ttttggaaaa gaaggctgtc ggttgatatc
     2521 cctgtaaata gtctttccaa aatacatccc cattagcggc aacggcattt cttcttatta
     2581 ccatgcaggt atccttatag gatggccgtt gcatggatac cggttccttg ccgccggtaa
     2641 gctgattata gttgtaaaaa aggtctttta gtaaaatagc ggtactccac ccatcgtata
     2701 gtatatgatg gtgggttatg ataaggacaa aagatcgatc ggccagttta acaacgtgca
     2761 gtcgcagggg cggcttagtc aggtcaaacc gttcatgcca gtccctgcgc aggtagtcct
     2821 ccacataacc gtaccggtct tttccgtcac gttgtgaaac atcggtgtat gcgataggaa
     2881 ttgcacaccg gtggaagatg atctgcaccg gcttgcttat ttcttcccat ctgaaaacgg
     2941 atcgtagaac ctggttcgcg gattgcaccc tgtcgaacgc atcctgcaat accgggacat
     3001 tcaaatggcc ttttatatcg aaggacaact ggacattata caggttctgg ttctcatctt
     3061 tcaggtaatg gtacaacatc ccttcttgaa ccatgttcaa ttcaacaatg tcttcgacat
     3121 ttgttctatc gatcttttgt tgcatagcaa gttatttaac cggcccgagg cggcggccgg
     3181 aa
//
LOCUS       DATGFG010001063         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_553602, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001063 DATGFG010000000
VERSION     DATGFG010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553602"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VKQ52_20190"
     CDS             complement(<1..796)
                     /locus_tag="VKQ52_20190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HLZ89590.1"
                     /translation="MMHIALLELSNKTVAADETSPAGSALSSRPSGPSDAGHGRQTRP
                     ATRLRGWLHLIPALALTLFLGLPAAKAQDPGFSQFFASPLTLNPALTGKFNGIVRVAG
                     NYRNQWPSINNAFITSTISVDAPIMTNRLPENDTWGIGIMAMTDKTASGILNSNYVSL
                     STAYHKSLDVDGYHQIGVGFQGTYANQTLDGTRLTFEDGLQLDGTWLRSPTEAINSQY
                     VAVHYFDANLGVLYNASTNGNNNFYVGASAYHLNHPKVSFLGVDTIN"
     gene            complement(1085..1735)
                     /gene="upp"
                     /locus_tag="VKQ52_20195"
     CDS             complement(1085..1735)
                     /gene="upp"
                     /locus_tag="VKQ52_20195"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HLZ89591.1"
                     /translation="MVVNLSEQYSLLSNWISEIRDEEIQKDRMRFRRNLERIGEVAAY
                     EISKTLPFEEREVQTPLGTATCKMLKDQPVITTILRAGLPLHQGLLNFFDRADNGFIS
                     AYRKHQRDGSFEISLDYVSCPELEGRVVIVSDPMLATGASLVKTIQYLREEGHPSEIH
                     VVVAIACTVGIEFVLRSEPQCKVWCGAIDDELTAKGYIVPGLGDAGDLAFGTKVQM"
     gene            2151..>2456
                     /locus_tag="VKQ52_20200"
     CDS             2151..>2456
                     /locus_tag="VKQ52_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89592.1"
                     /translation="MKKQWSILVGTALTVVMGASASSADGQAGPPRLDSAFVRFFSGE
                     WKGEGAFSNGRPITATVVFRLSLDSAWLVCEHRDVPPNSYKATLYWGVDRQTGQFEAY
                     "
BASE COUNT          445 a          559 c          957 g          495 t
ORIGIN      
        1 tattgatcgt atcgacgccg aggaaggaca ccttgggatg attgagatga taggccgagg
       61 ccccgacata gaaattgttg ttcccgttgg tggaggcgtt gtacagcacc ccgaggttcg
      121 cgtcgaaata gtgtacggcg acgtattggg agttgatggc ttccgtcggc gagcggagcc
      181 aggtgccgtc gagctggagc ccgtcttcga acgtgaggcg ggtgccgtcg agggtttggt
      241 tagcgtatgt cccctggaag cctacaccga tctggtgata gccgtccacg tccagcgact
      301 tgtggtaggc cgtggagagg gagacatagt tgctgttgag gatgccgctg gccgttttgt
      361 cggtcatggc catgatgccg atgccccagg tgtcgttctc ggggaggcgg ttggtcatga
      421 tgggggcgtc gacggatatg gtggaggtga tgaaggcgtt gttgatggag ggccactggt
      481 tgcgatagtt gccagctacg cggacgatgc cgttgaactt gccggtgagg gcggggttca
      541 gcgtgagggg ggaggcgaag aattgggaga agccggggtc ctgcgctttg gccgcgggga
      601 ggcccaggaa gagggtgagg gcgagcgccg ggatcaggtg cagccatccg cggagacggg
      661 tggcgggcct ggtttggcgg ccgtggccgg cgtcggaggg accggaggga cgggaggata
      721 aggcagaacc ggccgggctt gtttcatctg cggcgacggt tttgttggac agttctagca
      781 acgctatatg catcataaac agttatgggt ctctcgttat caaaagccgg atgggtgatc
      841 attgccgggt cagacctccc atttgcagac cttgcgtact aagaatagac gggaaaagtc
      901 ttcacacggt tgggtgatgg gggacaaatc gacgaaaaaa tagtaaaaaa ggatgagaaa
      961 gtgaagttaa agttgcaatg cgcgcccagg gcggtgttgc gaccggttgg cataggggcg
     1021 gacgtgaggg cgaacgaagc gatgcacgca ttcggcgtca aggggatggc acgggcacgc
     1081 ggccttacat ctgcaccttt gttccgaagg cgaggtcgcc ggcgtcaccg agaccgggga
     1141 cgatatagcc cttggctgtc aattcgtcat cgatcgcgcc gcaccagacc ttgcattggg
     1201 gttcgctgcg gaggacgaat tcgatgccga ccgtgcaggc gatggccact acgacgtgga
     1261 tctcggaggg gtggccttct tcccgtagat actggatggt cttgacgagt gaggcgccgg
     1321 tggcgagcat ggggtcggag acgatgacga cgcggccttc cagttcgggg caggagacat
     1381 agtcgaggct gatctcgaag ctgccatcgc gctggtgttt gcggtaggcg gagatgaagc
     1441 cgttgtcggc cctgtcgaag aagttgagca ggccctggtg caggggcagg ccggcgcgga
     1501 ggatggtggt gatcacgggc tggtccttca gcatcttgca ggtggccgtg ccgagcgggg
     1561 tctggacttc ccgttcctcg aaggggaggg tcttgctgat ctcgtaggcc gctacttcgc
     1621 cgatgcgctc gaggttgcgg cggaagcgca tccggtcttt ctggatctct tcgtcacgga
     1681 tctcactgat ccagttggat agcagcgaat actgctcgct aagattgaca accataaacg
     1741 ggatttgcgg gcgaaactac attatttatc ggagaacgcc gcacggttat gcagggttgt
     1801 cggcgcggcg gagagggcgg ggccgggagt ggggtggggg ttgtcggcgc ggtggcgccg
     1861 cggggggcgt gcgtgggacg gatggctaca cggaggggcg gtggcggcgc ggcgagaggg
     1921 gtgcagagat gtgaggaggt ccgcgggtga ggcgcgtgag gcggtggtgg cggctggcgt
     1981 gggatggatg gctacacgga ggggcggtgg cggcgcggcg atgtgaggcg gtccgcgggc
     2041 tcggcgggtg agccgtatgg ccggatggcc gatagccccg gatattgaaa aaccggcggc
     2101 attggttgaa tgagatttgt tacgggtgcg ggtgcaatta tattgggcgg atgaaaaagc
     2161 aatggagcat actcgttggg acggccctga ccgtagtaat gggggcatcg gcgtcatcgg
     2221 cagatgggca ggcgggcccg ccgcggctgg atagcgcgtt tgtgcgattc ttttccggcg
     2281 agtggaaggg agagggcgcc ttttccaatg gccggccgat caccgcgacc gtggtatttc
     2341 gtctgtcgct ggatagtgcg tggttggtgt gtgagcaccg ggacgtgccg ccgaattcgt
     2401 ataaggcgac gctctattgg ggtgtcgacc ggcagacggg gcagttcgag gcctat
//
LOCUS       DATGFG010001064         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_824169, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001064 DATGFG010000000
VERSION     DATGFG010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824169"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2266
                     /locus_tag="VKQ52_20205"
     CDS             <1..>2266
                     /locus_tag="VKQ52_20205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLZ89593.1"
                     /translation="QVLIGELLTIYESLRSGGGLPQAGAQDKYEDYIRYIEGRDKERE
                     ELHWRGYLSGLTSSSLLPFVGSMADRNKGVGVYRNEPLLLDKELTASVDRYAQVHRLT
                     VNTVMQGVWSYLLSRYTRSSRVTYGVTVSGRPEELTDVESRIGLYINTVPLHARIEAG
                     QETGAWLAGLQQGQLECREYQYTGLSTIQRWIGVQGDLFDSLLIFENYPVNTAISSRI
                     WSLQMEGVQVKEHTNYPLSIIIGGSEEMSIHFSYNSRLLEESYVRWIKGHFEEVLRQM
                     VSGRCATVGELRLLTLPEEKQLVERFNGTARAYPKDRSVVDLFEEQAEAIPGDTAIVF
                     ESAAGRRELTYGELNERANQLGHYLKRLGVQTEELVPICVERGVDMLVGMLGILKAGG
                     AYVPIDPEYPQDRIGYMLDDTGARMVLGSAATRSRLPMRERCRLVNLDEDWPDIGREP
                     ATRLPGLVAPGQLAYVIYTSGSTGRPKGVMIEHRGVVNLVYSQAAPLSLRRGICVFQF
                     ASFGFDASCYEIFCTLLHGGRLVLADKQTLLDPSVFLPVLIRQKVELIVLPTSYQSQL
                     KGDLIHLKTIVSAGEPLNAEIGKEIQRRGIKLINAYGPTENTVCAVLSEQPLRENGSI
                     TIGKPIDNVQIYIVDDDGRLQAVGVTGEICIGGAGLARGYLRREELTAAKFVPNPYSR
                     QADARMYKTGDWGRWLPDGNIEYLGRMDEQVKVRGYRIELGEIESVLQQSGLVSQGVV
                     LARGDSSGNKRLIG"
BASE COUNT          501 a          510 c          789 g          466 t
ORIGIN      
        1 tacaggtact gatcggagag ctgttgacaa tctatgagtc cctgcggtcg ggaggcggct
       61 tgccgcaggc cggagcgcag gataaatatg aagactatat ccgctatata gagggccgcg
      121 acaaggagcg cgaggagctg cactggcggg gctacctgtc cgggctaacc tcgagcagcc
      181 tgctgccgtt tgtcgggtcg atggcggatc gaaataaagg agtgggcgtt tacaggaatg
      241 aaccgctgct attggataag gaactaacgg catcggtaga ccgatacgca caggttcacc
      301 ggctgacggt gaatacggtc atgcaggggg tatggagtta cctgctgtcg agatataccc
      361 gcagttcgcg ggtcacgtat ggggtgacgg tatcggggcg cccggaagaa ttgaccgatg
      421 tggaaagcag gatagggctg tatatcaata ccgtgccgct gcatgcccgt atcgaggccg
      481 ggcaggagac cggggcgtgg ctggccggct tgcagcaagg tcagctggaa tgccgggagt
      541 accagtatac gggtctaagc acgatccagc gctggatagg agtacagggt gatcttttcg
      601 acagcctgct cattttcgag aactaccccg tgaatacggc gatctcctcg cgcatctggt
      661 cgttgcagat ggaaggggtg caggtgaagg aacataccaa ttatccgtta agcatcatta
      721 tcggcggctc ggaggagatg agtatccact tcagttataa cagccgccta ctggaagaaa
      781 gctatgtgcg atggataaag ggtcattttg aagaggtgct gcggcagatg gtgtcgggca
      841 gatgcgctac ggttggagag ctgcggctgc tgactttgcc ggaggaaaag cagttggtgg
      901 aaaggttcaa tgggacggcg agggcttatc cgaaggacag gtcggtagtg gatcttttcg
      961 aggaacaggc ggaggcgata ccgggcgaca cggcgatcgt atttgaatcg gcggcgggac
     1021 ggcgggagct gacctatggc gaactaaacg aacgggccaa ccaattgggg cactatttaa
     1081 agaggcttgg ggtgcagacg gaggagctgg tgccgatctg tgtagagcgg ggtgtggaca
     1141 tgctggtcgg gatgctggga atcctgaagg cggggggagc gtatgtaccg atcgacccgg
     1201 agtacccgca ggacaggatc gggtacatgc tggatgatac aggggcgagg atggtgcttg
     1261 gcagtgccgc tacccggtcg cggctaccca tgcgcgaacg ctgccggctg gtgaacctgg
     1321 atgaggattg gccggatatc ggccgggagc cggcaacccg tttgcccggt ttggttgcgc
     1381 ccggacagct ggcatatgtg atctatacgt cgggatcgac ggggcggcca aagggggtga
     1441 tgatcgaaca ccggggtgtt gtcaacctgg tatatagcca ggcggcgccc ttgtcgttgc
     1501 gcaggggtat ttgtgttttt caatttgctt cgtttgggtt cgacgcttcc tgctatgaga
     1561 tattctgtac gttgctgcat ggcggccggc tcgtgctggc cgacaagcaa accctgctgg
     1621 acccatccgt ctttttacct gtgctcatcc ggcaaaaggt ggagctgatc gtgcttccaa
     1681 cgagctatca atcccaattg aagggcgacc ttatccactt aaagacgatc gtgtcggcgg
     1741 gagagccgct caatgccgaa atcggcaagg agatacaaag gcggggtatt aaactgatca
     1801 atgcgtatgg tccaacagaa aatacggtgt gtgctgtttt atcggaacag ccattacggg
     1861 agaatgggtc cataacgatc ggcaagccca tcgataatgt tcaaatttat atagtagacg
     1921 atgatggccg tctccaggcg gtgggagtaa cgggcgagat ctgtatcggc ggcgccggtc
     1981 tggcccgtgg ctatttgcgc cgggaggagc tgacggcggc gaaatttgta cccaacccct
     2041 atagccggca ggcggatgcg cggatgtata agacgggtga ctggggtcgc tggcttccgg
     2101 acggaaatat cgagtacctg ggccggatgg acgaacaggt gaaggtccgg ggctatcgca
     2161 tcgaattggg tgagatagag agtgtgttgc agcagagcgg actggtgagc cagggggtgg
     2221 tactggcccg gggagacagt agcgggaaca agcggctgat aggata
//
LOCUS       DATGFG010001065         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_746160, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001065 DATGFG010000000
VERSION     DATGFG010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746160"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VKQ52_20210"
     CDS             complement(<1..609)
                     /locus_tag="VKQ52_20210"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ89594.1"
                     /translation="MKKLLLPFLFFFPVFMATAQQEMPLYGTDPIPNSRPVANAETID
                     SSGHPVRYSYSKVSHPALTVWLPPAGKRTGTAVIVCPGGGYVHLAMTHEGTEVAAWLN
                     SLGITAFVLKYRLPSDETMVDKTIGPLQDAQRAIQLVRQRAAEWGVDPGKIGILGFSA
                     GGHLASTAGTHFAKPVIANPGGVSLRPDFMVLLYPVISFDDNI"
     gene            complement(687..1736)
                     /gene="queA"
                     /locus_tag="VKQ52_20215"
     CDS             complement(687..1736)
                     /gene="queA"
                     /locus_tag="VKQ52_20215"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HLZ89595.1"
                     /translation="MKLSQFKFDLPLNLIAQHPAKKREDSRMMVVHRKTGQIENKHFR
                     DILDYFDDKDVFVVNNTKVFPARMYGRKEKTGAKIEVFLLRELNKPNRLWDVIVDPAR
                     KIRVGNKLYFGDNDELVAEVIDNTTSRGRTIRFLWDGNDEEFRQVLEILGETPLPKYI
                     KRKPDEEDKERYQTVYAKHEGAVAAPTAGLHFSKELIKRLEIKGVRFSEITLHTGLGT
                     FRPIEVEDLSKHKMDAEYYRIEEDACKIVNKARIGGHRICSIGTTTMRAIESSFTAEK
                     LLKPSEGWTNTFIHPPYQFNIADALVTNFHLPKTSLLIMVCAFAGYDLAMESYKKAIK
                     DKYRFFSYGDAMLIL"
     gene            2267..>3557
                     /locus_tag="VKQ52_20220"
     CDS             2267..>3557
                     /locus_tag="VKQ52_20220"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HLZ89596.1"
                     /translation="MDPLPAPPRMDKKYPALLWEITGNGLKKPSYLFGTMHVSSKLVF
                     HLSDSFYLDIRNAEVVALELDPQLWQDQLFRFENLQSNLRFYTQGAPNDLINEKSFQL
                     ERYEDRLKSALSEEPTVINGLLYRTYRTQADFEEDTYLDLYIYQTGKKLGKQATGVEN
                     YFQTERLVMEATQDMLKDKKKKAPDTDGESAYDIERKTQEAYRKGDLDLLDSLERIME
                     PSESYMEKFLYRRNEIQAASIDSIVQRHSLFVGVGAAHLPGRRGVIELLRKKGYTLRP
                     VVMPDRDATRRDDIDRIKVPVTFSTFTSEDGDFSVQLPGKLYRRSDSRSPDSWQYADM
                     GNGAYYMIGRVKTHSSFLGQKEETVLRKADSLLYENIPGKILKKTPVTRNGYRGFDIT
                     ARTRRGDIQRYNILVTPGEVWVFKMSGTNNYVEGPEAE"
BASE COUNT          861 a         1088 c          867 g          741 t
ORIGIN      
        1 gatgttgtcg tcgaagctga tcacggggta caacaaaacc atgaagtccg gtcgcaacga
       61 aaccccgccg ggattggcga tcaccggctt cgcaaaatgt gttcccgccg tcgaggccag
      121 gtggccgccc gccgagaacc ccaatatccc tatcttaccc gggtcgacgc cccactcggc
      181 ggcccgctgc cgcaccagtt ggatcgcccg ctgagcgtcc tgtaatggcc cgatcgtctt
      241 gtcgaccatc gtttcgtcac tgggcaggcg gtatttgagg acaaaggccg tgatcccaag
      301 actgttgagc catgcggcca cctccgtccc ttcatgtgtc atcgccaggt gcacatagcc
      361 gccgccggga caaacgatca ccgccgtacc tgtccgctta cccgccggcg gcagccatac
      421 cgtcagggca gggtggctca ccttgctgta cgaataacgg acggggtgac ccgaagaatc
      481 gatggtctcg gcattggcta ccggcctcga attggggatc gggtccgtgc catataacgg
      541 catttcctgc tgtgctgtag ccataaaaac gggaaaaaag aaaagaaagg gaagaagaag
      601 ttttttcata tgtcccctaa atataaaaaa aaagcagccg tctggaaggg aaaagtagac
      661 ggctgccggg tttctcttag aagatcttat aatatcagca tcgcatcccc atagctaaag
      721 aaccggtact tatccttgat cgccttcttg tacgactcca tcgccaggtc ataccccgca
      781 aaggcgcaaa ccatgatcag cagactggtc ttgggaagat gaaaattcgt caccagcgca
      841 tcggcgatat tgaactggta gggcggatgg ataaacgtat tggtccagcc ttcggaaggc
      901 tttaataatt tctcggcagt gaacgaggac tctatcgccc gcatcgtcgt cgtcccgatg
      961 gagcagatcc ggtgcccgcc aatccgcgcc ttgttcacga tcttgcaggc atcttcctcg
     1021 atgcggtaat attccgcatc catcttgtgt ttgctcaggt cttccacctc gatgggccgg
     1081 aacgtgccaa gacccgtatg aagcgtgatc tcggagaagc gcactccttt gatctccaaa
     1141 cgcttgataa gctccttgct aaaatgcagc cccgccgtcg gcgcggcaac cgccccctca
     1201 tgcttggcgt acaccgtctg gtaacgctcc ttgtcctctt cgtcgggttt gcgcttgata
     1261 tacttcggca acggcgtctc tcccaggatc tccagcacct ggcggaactc ctcgtcattc
     1321 ccatcccaca ggaaacggat cgtccggccc cggctcgtgg tattatcgat cacttccgca
     1381 accagctcgt cattgtcccc aaaataaagc ttgttaccga cacggatctt ccgggccgga
     1441 tcaacgatca catcccaaag ccggttcggc ttgttcaatt cacggagcag gaaaacttcg
     1501 atcttggcgc cggtcttctc cttgcggcca tacatgcggg cgggaaaaac cttggtattg
     1561 ttgactacaa aaacatcttt gtcgtcgaag tagtcgagaa tgtcccggaa atgtttgttc
     1621 tctatctggc cggtcttgcg atgcacgacc atcatacggc tgtcttctct ctttttcgca
     1681 gggtgctggg caataaggtt cagagggaga tcaaacttga actgtgaaag cttcatgtgg
     1741 aatgttgatt ttttattata tcttctcgat caaatcatca gcgcatacga ttcacacatg
     1801 ataccagggg gaggtaaaaa tagataatct cgagccaatc cgcacaaacc aaaccaggca
     1861 aagggcgcct ccggcgacca agccgaaggc caaacataag ccccccggca cccgatctgc
     1921 catcatgtta cggcatgatt tttcaagcgg gcgaaggtat gacaaaaact aagcatttcc
     1981 aaatatagtt tttggcgcct ccccttaccc cacggcaacc cgcgtgcata caacaacaat
     2041 cccacccggc cgacggttgt aaaagtcccc gaaatttgct caccccccgg taaaataatt
     2101 ttcccgaatt gccgtctttt atgcaatttg tagcgtatcg gatagtaagt atataacctc
     2161 cctgataatg agatctaccc cgcttaggtt ctgtgtgccg gcaaccgtat ttttccttct
     2221 cctggcccta accggatgga agccggtttg cgcaaaatgc gccgacatgg atcccctccc
     2281 tgcgcccccc aggatggaca agaaataccc ggccctctta tgggagatca ccggcaacgg
     2341 cctgaaaaaa ccttcctatc tcttcggtac gatgcatgtc agcagcaaac tggtcttcca
     2401 cctcagcgat tccttctatc tcgatatccg caatgccgaa gtggtcgccc tcgaactcga
     2461 cccccagctc tggcaggatc agctcttccg gttcgagaac ctccagtcca acctccgttt
     2521 ctatacccag ggcgcgccaa acgacctgat aaacgagaag tccttccagc tcgaacgtta
     2581 tgaggaccgg ctcaaatccg ccctcagcga agaacccacc gttatcaacg gcctgctcta
     2641 caggacctac cgtacccagg ccgatttcga agaagatacc tatctcgacc tgtatatcta
     2701 ccagacgggt aagaagctag gcaaacaggc caccggcgtg gagaactact tccagaccga
     2761 acgcctcgtc atggaagcca cccaggacat gctaaaagac aaaaagaaaa aagcaccaga
     2821 tacggacggg gaatccgcct acgacatcga acgcaagacc caggaagcct accgcaaagg
     2881 cgacctcgat ctcctcgact ccctcgaaag gatcatggag ccctccgaat cctatatgga
     2941 aaaattcctc tatcgccgca acgagatcca ggccgcctcc atcgacagca tcgtccagcg
     3001 ccattcgctc ttcgtcggcg taggagcggc ccacctccct ggcagaagag gggtcatcga
     3061 gctgctccgc aaaaaaggct ataccctccg ccccgtcgtc atgcccgacc gcgacgccac
     3121 ccgccgggac gatatcgacc ggataaaggt gcccgtcacc ttctccacct tcacttccga
     3181 ggacggggat ttctccgtac agctgccggg caaactctac cgccgcagcg acagccgctc
     3241 acccgactcc tggcaatatg ccgatatggg caacggcgcc tactatatga tcggccgcgt
     3301 aaaaacccat agcagcttcc tgggccagaa ggaggagacg gtgctgagaa aagcagacag
     3361 cctgctgtac gagaacatcc cggggaagat ccttaaaaag actcccgtta cccgtaacgg
     3421 ctacagaggc ttcgacatca ccgcccgcac ccgtcgcggg gatatccagc gctacaatat
     3481 cctcgtgacc ccgggggagg tatgggtctt caagatgagc ggcaccaaca actatgtcga
     3541 aggcccggaa gccgaac
//
LOCUS       DATGFG010001066         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1591239, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001066 DATGFG010000000
VERSION     DATGFG010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591239"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..829)
                     /locus_tag="VKQ52_20225"
     CDS             complement(68..829)
                     /locus_tag="VKQ52_20225"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HLZ89597.1"
                     /translation="MYKYLLLLLGILPFVPDQRPTLYIIGDSTVMNGKGRGDGSLWGW
                     GSFLDQSFDTTKIAVHNHALGGRSSRTFITEGHWDQVLSQLKPGDYVIMQFGHNDGGP
                     LDDTARARGTIKGIGDDSSNIYNPIMKKNETVHTYGWYMRKYVADTKAKGATAIICSP
                     IPRNMFKDGKVLRASVDYGKWAQETAKATDAYFIDLNVIIADQYDQLGPDKVKDFFPG
                     DHTHTNEAGARLNANAVVTGLRRLPNLPLTNYLRP"
     gene            complement(840..>2179)
                     /locus_tag="VKQ52_20230"
     CDS             complement(840..>2179)
                     /locus_tag="VKQ52_20230"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HLZ89598.1"
                     /translation="PDWTTKVGARTLPASKTIFTVTGADNSGATLATKFIQSTIDACA
                     AKGGGIVTLAPGKYLTGSLFLKDGVTLRLDKDVELLGSQHFEDYPDIGTRIAGIGMQW
                     PAALLNIIGAKKAAITGEGTVNARGKFCWDKYWSMRKTYDPKGLRWIVDYDAKRVRTL
                     LVQDAEDITIQGLKFIDAGFWTVQVLYSKYVTVNGITVRNNENGHGPSTDGVDIDSSS
                     WVLVENCDIDCNDDDFCLKAGRDWDGLRVNRPTEYVVIRHCTARKGGGLLTIGSETSG
                     GIRHVLATDLTAKGTGNGFHIKSATTRGGTVEDIHIRNIHMDSVGNAILFTMNWNPAY
                     SYSTLPAGYNPDSIPAHWKTMLHKVEPAEKGIPHFKDIYVSNITVANAKKAISATGLE
                     QSLITGVHIDNTTINATTAGDISFAKDWQLEKLSVTTKDGSHIQVKNSTGINL"
BASE COUNT          371 a          542 c          743 g          523 t
ORIGIN      
        1 cggagggtat ccgccgcgtg tgtagtggcg cagaggacca ggaacgctat gactgcgaag
       61 aggcgcatca ggggcggagg tagttggtga gcgggaggtt gggaagccgg cggagaccgg
      121 taacgacggc attggcattc agcctggccc cggcctcgtt ggtatgggta tgatcgccgg
      181 ggaagaaatc cttcaccttg tcgggaccca gctgatcata ctgatccgcg atgatgacgt
      241 tgagatcgat gaagtatgcg tcggtggcct tcgcggtttc ctgcgcccac ttaccgtagt
      301 cgaccgaagc gcggagcacc ttaccgtcct tgaacatatt gcgcgggata ggtgagcaga
      361 tgatggccgt tgcgcccttc gcctttgtgt cggcgacgta cttgcgcata taccagccat
      421 aggtgtggac ggtttcgttc ttcttcatga tcgggttgta gatattgctg gaatcatcgc
      481 cgatgccttt tatcgtgccc cgcgcccggg cggtatcatc cagcggtccg ccgtcgttat
      541 gcccgaattg catgatgaca tagtcgccgg gttttagctg ggagagcacc tgatcccagt
      601 gtccttcggt gataaacgtg cggctgctgc gaccgccgag cgcgtggttg tggacggcga
      661 tcttcgtggt gtcgaaggac tggtcgagga aactgcccca gccccagagg ctgccgtcgc
      721 ccctaccctt tccgttcatg acggttgagt cgccgatgat ataaagtgtg ggccgttgat
      781 cggggacgaa ggggagaata ccgagcagga ggagaaggta cttgtacata ttgaagatat
      841 taaaggttta tgccggtgga atttttgacc tgtatgtggg agccatcctt tgtggtaacc
      901 gacagttttt ccaactgcca gtcttttgca aagctgatgt cgccagcggt ggtggcgttg
      961 atagtggtat tgtcgatgtg caccccggtg atcagcgatt gctccagtcc cgtggcggag
     1021 atggcttttt tcgcgttggc tacggtgata ttggagacat agatatcttt aaaatggggg
     1081 atgcctttct ccgcgggctc tactttgtgc agcatcgtct tccagtgggc ggggatcgag
     1141 tcggggttat aaccggcggg gagggtcgag tagctgtagg cggggttcca gttcatggtg
     1201 aacaggatgg cgttgccgac gctgtccata tggatattgc ggatatggat gtcttccacc
     1261 gttccgcccc gggtcgtcgc cgatttgata tggaagccgt tgccggtgcc tttcgcggtg
     1321 aggtcggtgg cgagtacatg acggatgccg ccggaggtct cgctgccgat ggtgagcagg
     1381 ccgccgccct tgcgggccgt gcagtgacgg atgacgacgt attcggtagg gcggttgacg
     1441 cggaggccgt cccagtcgcg gccggccttg agacagaagt catcgtcgtt gcagtcgata
     1501 tcgcagttct ccaccagcac ccaggaggag gagtcgatgt cgacgccgtc ggtgctgggg
     1561 ccatggccgt tctcgttgtt gcggaccgtg atgccgttga ccgtgacgta tttcgagtag
     1621 agcacctgta cggtccagaa gccggcgtcg atgaatttga gtccctggat ggtgatgtct
     1681 tccgcatcct ggacgaggag ggtgcggacg cgtttggcgt cgtagtcgac gatccagcgc
     1741 agccctttcg gatcataggt cttgcgcatg ctccagtatt tatcccagca gaatttgccg
     1801 cgggcgttta ccgttccttc gccggtgatg gcggcttttt ttgcgccgat gatgttgagg
     1861 agggcggcgg gccattgcat cccgatgccc gcgatgcggg tcccgatatc agggtagtct
     1921 tcgaagtgct ggctgccgag gagttcgacg tccttgtcga ggcgaagggt gacgccgtcc
     1981 ttcaggaaga gggagccggt taggtatttc ccgggagcga gggtgacgat gccgccgcct
     2041 tttgcggcac aggcgtcgat cgtggactgg atgaattttg ttgcgagggt tgcgccgctg
     2101 ttatccgccc cggtgacggt gaagatcgtt tttgacgcgg gcagcgtacg ggcgccgacc
     2161 tttgttgtcc agtcggggt
//
LOCUS       DATGFG010001067         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_530630, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001067 DATGFG010000000
VERSION     DATGFG010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530630"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..783
                     /locus_tag="VKQ52_20235"
     CDS             88..783
                     /locus_tag="VKQ52_20235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ89599.1"
                     /translation="MNTLIIDDNAIARSVLKKLASQVKEITIAGECATAMEAYNLLQE
                     QAVDLLLLDIEMPGMSGIQLVRDLGAHRPVIIFTTSKKDYASEAFDLNVADYIVKPIT
                     PARFHQAIGKAREIIESNQEEVKWTAEEFLFIRDSAIIRRLKVDDILFVEAMGDYVKL
                     HTPQKFYAVHTTLKVVEDRLPSTRFLRVHRSYLVALNKIDTIQEGVLIIYDKPIPVAD
                     AYRKSLNQRMNIL"
     gene            906..1109
                     /locus_tag="VKQ52_20240"
     CDS             906..1109
                     /locus_tag="VKQ52_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89600.1"
                     /translation="MEIIYALVFFLDLLAICGLGFLSFREIDNRGPAWDQALLVIALV
                     LAIVVMVLFIRHYLRRDTGPNDN"
     gene            1128..>2822
                     /locus_tag="VKQ52_20245"
     CDS             1128..>2822
                     /locus_tag="VKQ52_20245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ89601.1"
                     /translation="MASNRFIYIILAAFITGMLLLVFIQYNSSRSIDQLIEGNLLLLN
                     DMKLGNDLREMERDLLTVESRVRAAVATGDSSFLQGSDDRIADAASNLDTLRRMSQDT
                     ASTNDIDRLSVLAKERLDRKNFLVDSFFRMGRRPPPSIIANTRTREAANEINTIMRRL
                     YARRKERLAAISASVEKYGRVARTSGIILITGVFFSAGGLFWFIIDRIRKQRLLICKL
                     DASERKLQVAVRIKENFLANMSHEIRTPLNSIIGFTGLLNRQSLNEPSREFVTAIQSS
                     GENLLAIVNDILDLSKLEAGAMRVEHTPFSIRELIHSVDTLFRHRVREKGLAFEAVVG
                     AGVPDILLGDANRLTQILVNLIGNALKFTESGGITVHATAEDILPQSLRLSIEVSDTG
                     IGIQPQQQEAIFDRFSQAESSITRKYGGTGLGLAIAKELIDIQHGAIEVESQPGMGTT
                     FRLSIPYPIVAAAGSSPISPSQPAVPAGQHTGKAHILVADDNRMNQRLMEHLLAAQGI
                     TFDLVENGRQAIEYLRQKTYHLVLMDIRMPVMDGYRASRLIREELRSSVPIVAMTAH"
BASE COUNT          637 a          823 c          763 g          599 t
ORIGIN      
        1 ccgggggcaa tcgcccaaca ggcaaaaact gtcgtcggat ccaaaacgcc ggccggccaa
       61 cataaaaatt tattctacat ttaaggtatg aacaccctca tcatcgatga caacgccatt
      121 gcccggtcgg tcctcaaaaa actggcctcc caggtgaagg aaatcactat cgcaggtgaa
      181 tgcgccacgg cgatggaggc gtataacctc ctgcaggagc aggcagtcga tctcctcctg
      241 ctggatatcg aaatgcccgg tatgtcgggt atccagctgg ttagggatct cggcgcccat
      301 cgccccgtca tcatcttcac cacctcaaaa aaagattatg ccagcgaggc tttcgatctc
      361 aatgtcgccg attatatcgt caagcccata acgcccgccc ggtttcacca ggcgatcggc
      421 aaagcccggg agatcatcga aagcaaccag gaggaggtga aatggacagc cgaggaattc
      481 ctcttcatcc gcgacagcgc catcatccgg cgcctgaagg tggacgatat cctgttcgtg
      541 gaggccatgg gcgattatgt aaagctccat acgccccaaa aattctatgc tgttcatact
      601 accctgaagg tggtggaaga caggctgccg tccacccgct tcctccgggt ccatcgctct
      661 tatcttgtcg cactcaataa gatcgatacg atccaggaag gggtccttat catttatgac
      721 aagccgatcc ccgttgcgga tgcctatcgc aaatcgctca accagcggat gaatattttg
      781 tagacgccgt taccccgtac gacatgatca ccagcccgtc gatggctttt cctcttccgc
      841 cgacgattct tgccgatttg gcgattgcgt acccccgcaa gaggaggtgg gctgaccttt
      901 gtgccatgga gatcatttac gcccttgtct tttttctgga cttgctggcc atctgtggac
      961 tgggtttttt gtcgttccgg gagatagaca acagaggccc ggcctgggac caggccctac
     1021 tggtcatcgc cctcgttctg gccattgtgg tgatggtcct gttcattcgg cactatctgc
     1081 gccgcgatac ggggccgaac gataattgag taacttacac atccaatatg gccagcaacc
     1141 gtttcatcta tatcatctta gccgccttca tcaccgggat gctgctgctc gtgttcatcc
     1201 agtacaattc atcgcggagc atcgatcagc tgatcgaagg caacctgctg ttgctgaacg
     1261 atatgaaact gggcaacgat ctgcgcgaga tggagcgcga tctccttacc gtggaaagca
     1321 gggtccgggc ggcagtagcg acgggcgact cttccttcct gcaaggctcc gatgaccgga
     1381 tagccgatgc agcatccaac ctggatacgc tgcggcgaat gagccaggat acagcgtcca
     1441 ccaatgatat cgatcggcta tcggtgctgg caaaggagcg gctggacagg aagaacttcc
     1501 tggtcgacag cttttttcgc atggggagaa ggccgccacc atctatcatc gccaatacaa
     1561 ggacgagaga ggcggccaat gagatcaaca ccattatgcg gcggctgtat gccaggcgga
     1621 aagagcggct ggcggcaatc agcgcttcgg tggagaaata cggccgcgtc gcccgtacca
     1681 gcggcattat cctcataaca ggagtcttct tcagcgccgg cggcttgttc tggttcatca
     1741 tcgatcgcat ccggaaacag cgcctactca tctgcaagct tgatgcctct gaacggaaat
     1801 tacaggtagc ggtgcggatc aaggagaact tcctggccaa tatgagccat gagatccgga
     1861 ccccgctgaa ttctatcatc ggattcaccg gcctgctcaa caggcaatcg ctgaatgagc
     1921 catcccgcga attcgtcacc gccatccagt cgtcgggaga aaacctcctg gcgatcgtca
     1981 acgatatcct tgacctgtcc aagctcgaag cgggtgcgat gcgggtggag cataccccgt
     2041 tcagcatccg ggagctgatc cattccgtcg ataccctttt ccgccatcgg gtgcgggaaa
     2101 agggcctggc attcgaagcc gtcgtcgggg ccggagtgcc ggatatcctg ctgggcgacg
     2161 ccaaccggct gacgcagata ctggtcaacc tcatcggaaa tgcattgaaa ttcaccgaat
     2221 ccggtggcat caccgtgcat gccacggcag aggacatcct ccctcagagt cttcgattgt
     2281 ctatcgaagt cagcgacacc ggcatcggta tccaaccgca gcagcaggaa gctattttcg
     2341 accggttttc ccaggcggaa agctccatca cgcggaaata cgggggcacg ggactcgggc
     2401 tggccatcgc aaaagagctg atagatatcc agcacggagc catcgaagta gagagccaac
     2461 ccgggatggg gactactttc cggttgtcga tcccctatcc tatcgtggcg gcggccgggt
     2521 cttcgcccat ctccccgtcg cagcctgctg ttcctgccgg acaacacacc ggcaaggccc
     2581 atatccttgt cgccgatgac aaccggatga atcagcggct gatggaacat cttctggccg
     2641 cacagggcat caccttcgac ctcgttgaaa acggccggca ggcgatcgaa tatctccgac
     2701 aaaagaccta tcatttggtg ctgatggata tccgcatgcc ggtgatggat gggtatcggg
     2761 ccagccgcct cattcgtgaa gagctgcggt cttctgtgcc catcgtggct atgacagcgc
     2821 ac
//
LOCUS       DATGFG010001068         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1430460, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001068 DATGFG010000000
VERSION     DATGFG010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430460"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2090
                     /locus_tag="VKQ52_20250"
     CDS             <1..>2090
                     /locus_tag="VKQ52_20250"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HLZ89602.1"
                     /translation="DMVVNNINDEALLYRNTSRDRDTATTHFLQIRYKGEARNRNGLG
                     AVATIYYDHGKQQAYENNPYRGYLSSMETITHFGLGRIRLIDSLVIRWPNGMQQTLAG
                     VQADQAITVSIADAKTPYSLQQPTVAAGALFKEVTAEKGIHYRHHDYDLIDFNFQHLL
                     PHKLSEYCPALAAGDLDGNGLDDLVIGGNGVYHTHVLLQQPNGQFLQKDSLPGNNLPS
                     KLTKDEGILIFDANGDGKPDLYIASGGYEFPSGSPNYEDRLYINDGKGNFSRDSTALP
                     LNYTSKFCVRAMDYNHDGKPDLFVSGRVDPWNYPRPVSSFIFRNDTQNGHVKFTDVTA
                     EVAPDLQQMGMICDALFTDFDGDGQTDLIVVGEWSPVSFLRNVNGKFVNVTNTTGVGN
                     QPGWWNSIVAGDFRHVGRMDYIVGNTGLNSLYKASDQYPVYMTAKDFDNNGRYVGIMS
                     LFLPDQHQNGEKHEYPAQARDDITNQLSSMKRKFSNYKSYAVATMDDVLSPEQRKGAL
                     RLKATMLQSCYLRNEGGGKFTMIPLPHLAQVSVVNGMVVDDFDGDGKLDVLINGNDFG
                     TAIGIGRYDASYGLLLKGDGAGGFTPLSIDQSGICIPGNGKALVKLRGADGSYLVAAS
                     QNKDVLKLYQLHRPTRSIPPGTGDVSALLRFANGKQQKEEFYYGSSFLSQSSRFLSVD
                     STVVSVTITDNKGR"
BASE COUNT          493 a          620 c          572 g          405 t
ORIGIN      
        1 tcgatatggt ggtcaacaat atcaacgatg aagcccttct ctaccggaat accagcaggg
       61 acagggacac cgcgacgaca catttcctgc agatcaggta taaaggggaa gcccggaaca
      121 ggaacggcct gggtgccgtt gccaccatct attacgatca tgggaaacaa caggcctatg
      181 agaacaatcc ctaccgggga tacctgtcca gtatggaaac catcacccat ttcggcctcg
      241 gcaggatcag gctcatcgat tcactggtga tccggtggcc caatgggatg cagcagaccc
      301 ttgccggggt gcaggccgac caggcgatca ccgtcagcat cgccgatgca aagaccccct
      361 attcccttca gcagccgacg gtggccgccg gggcattatt taaagaagtt acggcggaaa
      421 aaggcatcca ctatcgccac cacgactacg accttatcga cttcaatttc cagcatctcc
      481 tgccgcataa actgtcggaa tactgccccg cactggccgc gggcgatctc gacggcaatg
      541 gtctcgatga cctggtcatc ggcggcaacg gcgtctatca tacgcatgtg ctgttacaac
      601 aacccaatgg tcaattcctg caaaaggatt ccctgccggg gaataacctg ccctccaaac
      661 tgacaaaaga cgaaggcatc ctgatcttcg acgcgaacgg cgacggaaag ccggacctgt
      721 atatcgccag cggcggatac gaattcccct ccgggagtcc caactacgag gaccggctct
      781 atatcaacga cggcaagggg aatttcagcc gggactccac ggccttgccc ctcaactata
      841 ccagcaaatt ctgcgtccgc gcgatggact ataaccacga tgggaaaccg gacctcttcg
      901 tctccggccg cgtggacccc tggaattatc cgcggcccgt cagcagtttt atcttccgca
      961 acgatacgca gaacggccat gtcaaattca ccgatgtcac ggcggaagtg gcgcccgatc
     1021 tgcagcagat gggcatgatc tgcgacgccc tgttcaccga tttcgacggc gatgggcaaa
     1081 cagacctgat cgtcgtcggc gaatggtcgc cggtcagttt tctgcggaat gtgaacggga
     1141 aattcgtcaa cgtcaccaac acaaccggtg tgggcaatca gccgggctgg tggaactcca
     1201 ttgtagcggg ggacttccgg catgttggcc gcatggatta catcgtgggg aacacggggc
     1261 tgaacagcct gtataaggcc agtgaccagt atccggtcta tatgaccgca aaggacttcg
     1321 acaacaatgg ccgctatgtc ggcatcatgt ccctgttcct acccgaccag catcagaatg
     1381 gagagaagca cgaatacccg gcccaggcca gggatgatat taccaaccag ctcagcagca
     1441 tgaaacggaa attttccaac tacaagtcct atgccgttgc caccatggac gatgttctgt
     1501 cgccggaaca gcggaagggc gcccttcggc tgaaggccac gatgctccaa tcctgttatc
     1561 tgcgaaacga gggcggcggg aaatttacca tgatcccact cccccatctc gcccaggtgt
     1621 cggtcgtcaa cggaatggta gtggatgatt tcgacggcga tggcaaactg gacgtgttga
     1681 tcaacggcaa tgattttggc acggcgatcg gcatcggccg gtatgacgcc tcctatggcc
     1741 ttttgttaaa aggcgatggc gccggcggtt ttactccctt gtccatcgac cagagcggca
     1801 tctgcatccc gggcaacggc aaggcgttgg tgaagctgcg cggcgcggac gggagctatc
     1861 tcgtagcggc cagccagaac aaagatgtct taaaactgta ccagttgcac cggccgacgc
     1921 gcagcatccc tcccggcacc ggcgatgtca gcgccctgct ccggtttgcc aatgggaagc
     1981 agcaaaaaga ggaattctat tatgggtcct ccttcctgtc gcagtcgtcc cggttccttt
     2041 ccgtggactc gacggtcgtc agcgtcacca tcaccgacaa caaaggcagg
//
LOCUS       DATGFG010001069         7936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1699587, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001069 DATGFG010000000
VERSION     DATGFG010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699587"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VKQ52_20255"
     CDS             <1..738
                     /locus_tag="VKQ52_20255"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ89603.1"
                     /translation="NAEIMFSGRYQIPNAYSPADYLYTYSSSFQPVSYLVNDYECTDG
                     LPITASPLYKAATPYNNRDPRLLATILTPGVLYKGGIPFVPAQIGASAGFLNKKGVDS
                     TRATVIGTQSDQDWVYLRYADVLLMYAEAENEVSGPAPSVYSAVNAVRARPSVNMPAL
                     PVGLSQDSMRTRIRHERRIELALEGQRYFDLKRWRLDRVIIPTIKDPSGAYRTFPLRD
                     TLWPVPQSEIDLSKTYNNPAFTQTPGY"
     gene            788..2137
                     /locus_tag="VKQ52_20260"
     CDS             788..2137
                     /locus_tag="VKQ52_20260"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLZ89604.1"
                     /translation="MESAQKKKLAGWGNYPVAESFTLCPRSEEDARGDLEKGKLVARG
                     LGRSYGDQAINDNNYVAICTKLDHFLNWDEKEGILECEAGVSLEEIISTFGPKGWLPM
                     ICPGTKFVTIGGAIANDIHGKAHHIDGSFVNCVLSFTILLADGTVLEASRTENADLFW
                     ANFGGLGLLGVILKARLKLRKIETTYFRQKSIRIKGLDHMLEALDHYDHEYNYSVAWI
                     DALAKGSKLGSGVLTLGNAAKLSDLPEKLKADALKLHATSKLAVPFFFPSFALNGLTV
                     RILNRVIAFVQNSPKEFVHYEKFFFPLDAINNWNKGYGKRGFIQYQFVIPEENGKKNL
                     TEILEMIAASGCTPFLNVFKRMGDGQGILSFPFKGYTLAIDFPVTKNLFTFTPKLDAK
                     VLAAGGRLYLGKDALLQESTFKAMYPQHEEWLAIKRKYDPGNRFSSNISRRLGLDLA"
     gene            complement(2181..2810)
                     /locus_tag="VKQ52_20265"
     CDS             complement(2181..2810)
                     /locus_tag="VKQ52_20265"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ89605.1"
                     /translation="MNQPEPQGSGKEHAPTPDITPLLTVLNYFHPLTSEMEAFLRTHV
                     TGLTVRKRKLLLKEGSFCEHVYFIVRGAVRGFTREGQKDITTWIVVENELVSSILSLN
                     SHEPSVENIQVLENSELLVISREDLERMYRELPEFNVVARKLLQHYYADAERRAFIAR
                     LTKAENKYRHFLLHHEPLSNRIPLKYIASYLGMTLETLSRVRKKFSSRI"
     gene            complement(2846..3475)
                     /gene="nth"
                     /locus_tag="VKQ52_20270"
     CDS             complement(2846..3475)
                     /gene="nth"
                     /locus_tag="VKQ52_20270"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HLZ89606.1"
                     /translation="MRAKIDWPVAIRPLLRKYKSKRHPLDYQNVYQLLVMVVLSARST
                     DDFVNAVAPKLFAAFPDMSSLAGVQPEALFPYIAGITNFAHKAKWLTAIAAEIKTDEQ
                     IPRSLEKLIQLPGIGRKSANVILREAGVPPEGVIVDVHVLRVAPRLGIAKGTDPKKVE
                     EHLMKILPPKEWDAGMAMSFLGREICTPKPKCDSCLMNKVCLYYSRLKK"
     gene            3568..5946
                     /locus_tag="VKQ52_20275"
     CDS             3568..5946
                     /locus_tag="VKQ52_20275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89607.1"
                     /translation="MIRNSLTIAWRSLSRNKRVAAINIFGLGLGITCSLLILLWVRDE
                     RKVDAFHANGSRLYQVYERSYFDGKADAGYATQGLLAEELKRVIPEIQYASGMDYVSA
                     PGTKATVAANNKVTKMTGFFAGKDFFTMFSYPLLQGSAAAALDEPGVIAISRDLAADF
                     FGSPENAIGKTILFNNEQSLKVTAVFENIPTHSSQQFDFLRSWADFVKANDWVNNWGN
                     ESPSSFIQLRAGADPVSVEGKIKDFVYRYMARSSGFRLELGLQSYPERYLYSSFKNGY
                     VDGGRIEYIRLFTLVAVFVLLIACINFMNLATAQSAKRAKEVGLRKVIGAARSSLIIQ
                     FIGEALLLTALSILLALALTALLLPAFNNLTGKQLTLPIAEPAFWMTIAGLLLITGLF
                     AGSYPALFLSSLNPVATLKGGLKFGWSALFFRKGLVVFQFTLSLILIVGMIIVNRQVT
                     YIRTKNLGYDRENLVYIPIEGELVKRYSLFKEEAGKLPGILNISKMRNSPTVIEHHTG
                     SIHWPGKDPNAAISFADGVVGYDFVKTMKLQLKEGRDFSTDFPSDSAGFLLNETAVNR
                     IGWQNSIGQTLDWGGHRGKVIGVLKDFHFNSLHQTIDPLIVRLDENWPWGTILVRTRA
                     GQTHEAIAALEKLCKDLNPEFPFTYQFSDLEFAHLYKSETTVGRLSDCFAFLAILISC
                     LGLFGLATFTAAQRTKEIGVRKVLGASTSNIALLLSGDFLKLITLAIGLAFPLAWLVM
                     HKWLQQFAYKIDIEWWVFALAGLATLGIALLTVSYQSIKAALANPVRALRSE"
     gene            6163..7386
                     /locus_tag="VKQ52_20280"
     CDS             6163..7386
                     /locus_tag="VKQ52_20280"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HLZ89608.1"
                     /translation="MKKGIVNVRVLPDSRRKKDGGKFPLKLRITHKGERRYYGTGFDA
                     NEEEWAAINSACPRGELQRIKAGVAEIEKKAEECAEKMVLFSFKQFEKDFFGGAIRFE
                     SLKSAFEAQIRQLEDNDQWGTASSYRTACNALHRFKAKITLEDVDQDFLQKFEKWVLK
                     EGMSITTVGIYLRALRTIINIAIDNGTFRKDAYPFGKRKYVIPTGRNIKKALAIDQIK
                     KIFEYPTPGGGFMEQAKDFWIFTYLCNGINMMDIARLKGKDIDAKAIRFVREKTKRTK
                     RGDPLIITAARNSHVNAILIKWGKKSLSAEEYVFSIIDNSDTPKIARAKIQQFTKLVN
                     KWMKRIGEDLGFDLTLTTYVARHSFATILLRSGAPMTFASQSLGHSNVITTQKYFAGF
                     DIEAQSEYIKALTNF"
     gene            7600..>7936
                     /locus_tag="VKQ52_20285"
     CDS             7600..>7936
                     /locus_tag="VKQ52_20285"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLZ89609.1"
                     /translation="MKQLLKKLESKPGFVASIEACEKEATPILQGFITNFPDYTDHSI
                     NHSKKVLAYSEFVLEDQLNKLNEDEVYILLMAGLLHDIGMCPTNKMKKKINESKEFLE
                     SNKSFETYLR"
BASE COUNT         2004 a         2109 c         2002 g         1821 t
ORIGIN      
        1 aacgcggaga tcatgttctc cggccgttac cagatcccca acgcctatag ccccgccgac
       61 tatctgtata cctatagctc cagctttcag ccggtaagct acctggtcaa cgactatgaa
      121 tgtaccgacg gcctgccgat caccgcttcg cccttataca aggcggccac gccctacaat
      181 aaccgcgatc cccgcctgct ggccaccatt ctgacgccag gggtgctata caagggcggt
      241 atacccttcg tccccgcaca gatcggcgcc tcggccggtt ttctgaacaa aaaaggcgtg
      301 gactccaccc gggccaccgt catcggcacc caaagcgacc aggactgggt ctatctgcgc
      361 tatgccgatg tcctgctgat gtatgcggag gcggagaatg aagtgagcgg accggctccc
      421 agtgtctaca gcgcggtaaa tgccgtccgc gcccggccga gcgtcaatat gcccgccctg
      481 cccgtcggat tatcacagga tagcatgcgg acccgcatcc ggcatgagcg gcgcatcgaa
      541 ctggccctgg aaggtcaacg ctatttcgat ctcaaaagat ggcggctcga ccgcgtcatc
      601 atcccgacga taaaagatcc gagcggcgct taccggacct tccccctgcg ggatacactg
      661 tggcccgttc cccagtcgga gatcgacctg tcaaagactt acaataaccc tgcctttacc
      721 cagacacccg gatattgata aactcgttga ctatgactaa tttcacggcg aattcaaacc
      781 gtttctcatg gagtccgccc agaaaaagaa gttagccgga tggggtaatt accctgtagc
      841 tgaatcgttt accctatgtc cccgcagcga agaagacgcc aggggcgacc tggagaaagg
      901 aaaactcgtc gcccggggat tgggcaggag ctatggcgac caggccataa acgacaacaa
      961 ttatgtcgcc atctgcacca aactcgatca tttccttaac tgggatgaaa aagagggcat
     1021 cctggagtgt gaagccggag taagcctgga agagatcatt tccacctttg gaccaaaagg
     1081 ctggctgccg atgatctgcc ccggaacaaa attcgtgact atcggcggcg ccatcgcaaa
     1141 cgatatccac ggaaaagcgc atcacatcga tggatcgttc gtgaattgcg tattgtcttt
     1201 taccatcctg ctggccgatg gaacagtact ggaagcctcc cgaacggaga atgccgatct
     1261 gttctgggcc aatttcggcg gactgggact cctgggggtg atcctcaagg cccggctaaa
     1321 gctgcgtaag atagagacga cctatttccg gcagaagtcc atcaggataa aagggctcga
     1381 ccatatgctg gaagccctgg atcactatga tcacgagtac aattattccg ttgcctggat
     1441 cgacgcgctg gccaagggca gcaaactggg aagcggcgta cttacccttg gtaatgccgc
     1501 caaactttcc gacctgcccg aaaagctcaa ggccgatgcg ctaaagctcc acgcaacgag
     1561 caaattggcg gtgcccttct tctttccctc cttcgcattg aacggcctta ccgtccgcat
     1621 cctgaaccgc gtcattgcct ttgtacagaa ctcgccaaag gaatttgtcc actacgagaa
     1681 attcttcttc cccctcgatg ccatcaacaa ctggaacaag ggctacggca aaagagggtt
     1741 tatccaatac cagttcgtga tcccggaaga gaatggcaag aagaacctca ccgagatact
     1801 cgaaatgatc gccgccagtg gctgcacccc cttcctgaat gtcttcaaac gcatgggtga
     1861 tggccagggc atcctgtcct ttccgttcaa gggatacacc ctggcgatag atttcccggt
     1921 gacaaagaac ctgttcacct tcacgcccaa gctggatgcg aaggttctgg cagccggcgg
     1981 ccgcctctac ctgggaaagg acgccctttt acaggaaagc accttcaagg ccatgtatcc
     2041 ccagcatgag gaatggctcg ccatcaaacg taaatacgat cccggcaacc ggttcagttc
     2101 gaacatctcc cggcggctgg gcctcgacct ggcataagcg gcatgaacgg atgcccgccc
     2161 gaatagggag cccccggggt ctatatcctg gaagagaatt tctttctcac ccggctcagg
     2221 gtctccagcg tcatcccgag ataagaagcg atatacttca gcgggatgcg attcgacagg
     2281 ggctcatgat gcaggagaaa atgccggtat ttgttttccg cctttgtaag cctggcgata
     2341 aaagcgcgcc gctccgcatc ggcataatag tgctggagta atttgcgggc gaccacgttg
     2401 aactccggta actccctgta catccgctcc aggtcttccc tgctgatgac aagcagctca
     2461 gaattctcca atacctggat attctccacg gatggctcat ggctgttcag actcaggata
     2521 gaagagacca gctcattctc cacaacgatc cacgtggtga tatccttttg tccttcccgc
     2581 gtaaaaccac gcaccgcacc ccttacgata aaatagacat gctcgcaaaa agagccctcc
     2641 tttaacagga gttttcgctt tctgacagta aggccggtga catgagttct caggaaggct
     2701 tccatctcgg aagttagcgg atggaaatag ttcagcacag ttagtaaggg ggtaatatca
     2761 ggggtaggcg catgctcctt gcccgaccct tgtggttcag gttgattcat tgttggggct
     2821 tggggtttag gtaaacaaat ataaactatt tcttcagccg ggagtaatag agacaaacct
     2881 tgttcatcag gcaggagtca cattttggct tgggtgtaca gatctctctt cccaggaagg
     2941 acatggccat gccggcatcc cattcttttg gcggaaggat cttcatgagg tgttcttcca
     3001 ccttctttgg atcggtcccc ttggcgatgc ccagcctcgg ggccacccgc aggacatgga
     3061 catccacgat caccccctcc ggcggcacac cggcttcccg tagaatgaca ttcgcggatt
     3121 ttctgccgat accgggtaac tggatgagct tttccaggga gcgggggatc tgttcatcgg
     3181 tctttatttc tgcggcgatg gccgtcagcc atttcgcctt atgcgcgaaa ttggtgatcc
     3241 cggcgatata ggggaaaagc gcttccggct gtaccccggc cagtgagctc atatccggga
     3301 aggcggcaaa aagctttggc gcgacggcat tgacaaagtc atcggtcgat cgcgcggaga
     3361 ggacgaccat gacaagcaat tgatagacat tctgataatc cagcggatgt cttttgctct
     3421 tatatttccg gagcaggggg cgtatggcaa ccggccaatc gatctttgcg cgcatgatgg
     3481 gataaaggtc caaaaaattc tccaggattc ggtgcaacct aatggacaaa atgggcgtca
     3541 tatcttccct gacaaacttc cccgcttatg atccgcaact ctctcaccat tgcctggcgc
     3601 agcctgagcc ggaataaaag ggtcgctgcc ataaacatct ttggcctcgg actcggcatt
     3661 acctgtagcc tgctgatcct tctttgggta cgggacgaac gtaaggtgga cgcgtttcac
     3721 gccaatggat cgcgattgta ccaggtgtac gaacgcagtt atttcgatgg gaaagcagac
     3781 gccggctatg ctacccaggg tttgctggcc gaagagctga aaagggtgat cccggaaatt
     3841 caatatgcca gcggcatgga ctacgtctcg gcgccaggga caaaggctac tgtcgcggca
     3901 aataacaagg tcacaaagat gaccggattt ttcgcgggga aagatttttt caccatgttc
     3961 agctatcccc ttcttcaggg gagcgcggcc gccgccctcg acgaacccgg cgtcatcgcg
     4021 atctcccggg atctggccgc tgattttttt ggcagccccg aaaatgccat cgggaaaacc
     4081 atccttttca acaacgagca gtcgctgaag gtaacggcgg tgtttgagaa tatccccacc
     4141 cattcttcgc agcaattcga ctttctgcgg agctgggccg attttgtaaa ggcgaacgat
     4201 tgggtgaata actgggggaa cgaaagcccc tcttctttta tccagttgcg tgcaggcgcc
     4261 gatccggtaa gtgtggaagg gaagatcaaa gactttgtgt accgttacat ggccagatcc
     4321 tccggcttcc ggctggaact gggcctgcag tcctacccgg aacgatatct ttattcttcc
     4381 tttaagaacg gctatgtcga cggcggccgg atcgaataca tccgcctctt tacccttgtc
     4441 gccgtctttg tcctcctcat cgcctgtatc aactttatga acctggcgac ggcgcaatcc
     4501 gctaaacgcg caaaagaggt aggtctccgg aaagtgatcg gcgccgcccg gtcctccctg
     4561 atcatccagt tcatcggcga ggcgctgctg ctaaccgccc tttccattct cctggccctt
     4621 gcactgactg ccctcctcct gccggcattc aacaacctga cgggaaaaca attgaccctg
     4681 ccgatagcgg aacccgcgtt ttggatgacg atcgcagggc tcttgctgat caccggtctc
     4741 ttcgccggca gctatcccgc cctcttcctt tcttcgctga atcccgttgc gacgttgaaa
     4801 ggcggcctga aattcggctg gtcagcgctc tttttccgga aaggcctggt ggtctttcag
     4861 tttaccctct ccctgatcct gatcgtggga atgatcatcg tcaatcgcca ggtgacatat
     4921 atccggacaa agaatttagg ttacgatcgc gaaaacctcg tgtatatccc gatagaaggc
     4981 gaactggtaa aaagatacag cctcttcaag gaagaggccg gcaagctccc cggcatcctg
     5041 aacatctcca aaatgcgcaa ctccccgacg gtgatcgaac atcataccgg aagtattcac
     5101 tggccgggga aagacccgaa cgccgccatc tcctttgccg atggggtagt gggctacgat
     5161 ttcgtaaaga ccatgaaact acagctaaaa gaaggccggg acttctcgac cgatttccct
     5221 tccgactccg ccggtttcct gctgaacgaa acggccgtca accgcatcgg gtggcaaaac
     5281 tccatcggcc agaccctcga ctggggcgga caccggggaa aggtgatcgg cgtcttaaaa
     5341 gactttcatt ttaattccct gcatcagacc atcgatccat tgatcgtccg gctcgatgag
     5401 aactggccct ggggcacgat cctcgttcgc acccgcgcgg ggcagaccca tgaggcgatc
     5461 gccgcactgg aaaaactttg taaggacctg aacccggaat ttccctttac ctatcagttc
     5521 tccgatctgg aatttgcgca tctgtataag agcgagacca ccgtcggccg cctttccgac
     5581 tgctttgcct tcctcgccat cctgatctcc tgcctgggcc tgttcggcct ggcgaccttt
     5641 accgccgccc agcgcacaaa ggagatcggc gtgcgaaagg tgctcggtgc ttcgacatcc
     5701 aacatcgccc tgttgctatc gggggatttt ttaaaactca tcaccctcgc catcgggctc
     5761 gcctttcccc tcgcctggct ggtcatgcac aagtggctgc agcagtttgc ctataagatc
     5821 gatatcgagt ggtgggtatt cgccctggca ggcctcgcca cgctgggcat cgccctgctg
     5881 acggtaagct atcaaagcat caaagccgcg ctcgccaacc cggtccgcgc tctacgcagc
     5941 gaataacgaa atgatttcct taagtgggcc ctgctgggca cgatccagcg accctctgat
     6001 tatgagaccg atttcccggc actttcagtc acttccagtt aggcccagtt gttatgtaaa
     6061 taaattattt ccattgactt aggggtcgga ttccgtaaat ttgttttgag tcatttttga
     6121 aaaacatgtc tcaaatcatg tatcaaatct aagccttatt ttatgaaaaa ggggatagta
     6181 aatgtaaggg tcctgccgga ctctaggagg aagaaggatg ggggtaaatt tccgctaaaa
     6241 cttcggatta cgcataaagg ggagcgacgg tattatggga cgggctttga tgccaacgag
     6301 gaagaatggg cggccatcaa tagcgcctgc ccgaggggcg aattgcagcg gatcaaggct
     6361 ggagtcgcgg aaattgagaa aaaggccgaa gagtgtgccg aaaaaatggt tctattttcc
     6421 tttaagcagt tcgaaaagga tttttttggc ggggcgatcc ggttcgagag cctgaaatcg
     6481 gcgtttgaag cccaaatacg gcagctggaa gacaatgacc aatggggtac ggcgtcttcc
     6541 tatcgcacag cctgcaacgc cctgcatcga tttaaggcca aaataacact ggaggatgtg
     6601 gatcaggact ttcttcaaaa gttcgagaaa tgggtactta aagaaggcat gtcgatcaca
     6661 acggtcggaa tctatttgcg ggccttaagg accatcatca atatcgccat agacaatggc
     6721 acattccgaa aggatgccta tcctttcgga aaaaggaagt acgtaatacc cacgggtagg
     6781 aacatcaaaa aagcactcgc aattgatcag ataaagaaga tatttgaata cccgaccccc
     6841 ggcggcggct tcatggaaca agccaaggat ttctggatct ttacctacct ctgcaatggg
     6901 atcaatatga tggatatcgc ccggctcaaa gggaaagata tagatgccaa ggccatccgg
     6961 tttgttaggg aaaagacaaa gagaacaaaa agaggtgatc ccctaatcat aaccgcggca
     7021 cggaattctc atgtaaacgc gatcctgata aaatggggaa agaagagtct ttctgcggag
     7081 gaatatgttt tcagcattat cgataatagt gacaccccta aaattgccag agctaagatt
     7141 cagcaattta caaagttggt taataagtgg atgaaaagaa ttggtgaaga tcttggcttt
     7201 gacttaacgt tgacgaccta tgtagcccga cattcctttg ccaccattct ccttcgaagt
     7261 ggggcgccaa tgacctttgc cagtcagagc ctaggacatt caaatgttat aacaacccaa
     7321 aagtatttcg caggattcga catagaagcg caatctgagt acataaaggc gttgactaat
     7381 ttttgatgct taaacgaatt gacataggct taattgtaag cgaagctttt tgcttgatga
     7441 gaaggccgca atcgataaca caactgtacc ctctgtcaag ggcaaaatag gtgttaatac
     7501 gggtgcaaaa tgtaataaaa tttattagta ttgagctaaa atttgtgatt ttagaaaacc
     7561 tgccgttcgc aaatcgcctg taactcctta aactaaacaa tgaaacaatt attaaaaaaa
     7621 ctggaaagca aacccggatt tgtagcgagt attgaagcat gcgaaaaaga agccacgcca
     7681 atattgcaag ggtttataac aaattttccc gactacacag atcactcgat aaatcattct
     7741 aaaaaggtac tggcttattc tgagtttgta ttagaggacc aactaaacaa attaaatgag
     7801 gatgaggttt acattttatt aatggccggg cttttacacg acattggaat gtgtccgaca
     7861 aataaaatga aaaagaagat caatgaaagt aaagaattcc ttgaaagcaa taaatctttt
     7921 gaaacatact tgcgag
//
LOCUS       DATGFG010001070         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1201448, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001070 DATGFG010000000
VERSION     DATGFG010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201448"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VKQ52_20290"
     CDS             complement(<1..288)
                     /locus_tag="VKQ52_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89610.1"
                     /translation="MAIVTNLDSDLQSGEESIEVSGARVHNLKNIDIRIPRNQLVVIT
                     GISGSGKSSLAFDTIYAEGQRRYMESFGAYARQFIGDMERPDVDKITGLSPV"
     gene            complement(407..1300)
                     /locus_tag="VKQ52_20295"
     CDS             complement(407..1300)
                     /locus_tag="VKQ52_20295"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HLZ89611.1"
                     /translation="MKTVTKWLAAATGATAMLFFFACHKENSSSSNPNIPKGQSQVSM
                     YMTDGPGEFFKVLIDIRQIAIEIDTATKQKDSDRDDQWDANFNGEHRDASHKSVLWDT
                     LSITPGVYDLLALRNGVDTLLATGMYPTGKILKIKVTIGSDNTVYIDSTTHFTLNFPG
                     SDTTHTFTVNVAREDVDDISDNEFKLWLDFNIDRSIFAGNGGFFLEPFFEVFNDVQRA
                     KVYGTVLPEGAGALVTGYMGTDTIYAVPFDDGNYKFRNVPTGTWSFNFKGRNGYKDTT
                     ISNIKVDSLASVKLPTITLHK"
     regulatory      1512..1648
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1817..3010
                     /locus_tag="VKQ52_20300"
     CDS             1817..3010
                     /locus_tag="VKQ52_20300"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HLZ89612.1"
                     /translation="MQATTQLIHSIPVDPATGAIAVPIYQTSTFVQEAPGVNKGYDYA
                     RTGNPTRATLESLVAQLEGGSTGAAFASGLAAIDAVVKLLEAGDEIIAVNDIYGGAYR
                     LFEQVYRKFGINTHYVDTSNPEAVFHALTPKTKLIWIETPTNPTLKITDIRAIAKIAR
                     AHRCLLCVDNTFASPALQQPLLLGADLVVHSGTKYLGGHSDLIAGLVVTADPELGQKI
                     KFLQNAGGAILGPFDSFLLIRGIETLHLRVRQHCASARAIAAFLEDHPAVNKVYYPGL
                     VKHPGHTVAAGQSKGFGGIVSFSLKEDTVAAATAFVTSTQLFKLAESLGGIKSLVSHP
                     AAMTHKTIPAERRKAAGVTDSLIRLSAGLEDTEDLIADLRQALDRLTVKAEVTGSRQK
                     LEKVI"
     gene            3031..>4218
                     /locus_tag="VKQ52_20305"
     CDS             3031..>4218
                     /locus_tag="VKQ52_20305"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HLZ89613.1"
                     /translation="MDTHKQLTIGLFGFGVVGEGLYKVLKQTPSLKASIRKVCIKHPG
                     KKREAPAELFTTDRDAILNDPEINVVVEVINESGPAFEIVSRALKSGKSVVSASKKMI
                     AEHLPELLALQKETSASFLYEAAACASIPVIRNLEEYYDNDLLHSIKAIVNGSTNFIL
                     TKMFEEKLDFQEALLLAQQLGFAESDPSLDVEGYDAVNKWTFLLTHAYGIIVEAKDLV
                     FNGIQHIQAGDAEVAREKRYDIKLVAQAKKLANGKVAAFVLPQFIRQDDHLAFVKNEF
                     NGVVIESGFADKQFFYGKGAGSFPTASAVLSDLSALRYEYKYEYKKLYHHTPHELTDD
                     FFVRVFLSFDDLKYIPKDRFQSIEEWHADNDRKYLVGVLPFRELKGHSWWRENGTSLV
                     LTPD"
BASE COUNT          974 a         1041 c         1239 g          964 t
ORIGIN      
        1 gacggggctg agaccggtga tcttatcgac atcgggacgt tccatatcgc cgatgaattg
       61 ccgggcgtag gcgccaaagc tttccatgta gcggcgctgg ccttcggcat agatggtatc
      121 aaaagcaagc gaggatttgc cgctgccgct gatgccggtg atcaccacca gttgattcct
      181 tgggatgcgg atgtcgatgt tcttcagatt atgtacccgg gccccggata cttcgatact
      241 ctcttcgccg gattgcaggt cggagtccaa attcgttacg atcgccatag tgtgctaagg
      301 taagaacaac caccggaaag aaaggtttac agcggggatt tttgctaaaa aatttgggcc
      361 ggggagaatc cccggcccaa gacaaggcta aacgattatg gttgaattac ttatgaagcg
      421 tgatggtcgg cagtttcacc gatgccaggg aatccacttt gatattggag atggtcgtgt
      481 ccttgtaccc gttgcgtcct ttgaaattga aggaccatgt acccgtcggc acgttgcgga
      541 acttataatt gccatcgtca aaaggtaccg catagatcgt gtctgtgccc atatagccgg
      601 tgacaagggc gccggcgcct tcggggagca ccgtaccgta gaccttggct ctctgcacat
      661 cattgaagac ctcgaagaaa ggttcgagga agaatccgcc attccccgcg aagatcgagc
      721 ggtcgatgtt gaagtcgagc cagagcttga attcgttgtc ggagatatca tcgacatcct
      781 cccgggccac gttgaccgtg aacgtgtggg tggtgtccga gccggggaaa ttcagcgtga
      841 aatgggtcgt gctgtcgatg tagacggtgt tgtcggagcc gatagtgacc ttgatcttca
      901 gtatcttacc cgtggggtac ataccagtgg caagaagcgt atccacgcca ttgcgcaggg
      961 ccagcagatc atagacgcca ggagtgatgc tgagcgtatc ccagaggacg gatttgtgac
     1021 tggcatcacg atgctcacca ttgaagttgg catcccactg atcgtcacgg tcggagtctt
     1081 tttgtttggt agcagtgtcg atctcaatgg cgatctgccg gatatcgatc aaaaccttaa
     1141 agaattcccc cggaccatcc gtcatataca tggagacctg ggattggccc ttgggaatgt
     1201 tgggattgga ggaagaagag ttttccttgt gacaagcaaa aaagaacagc atggcagtag
     1261 cgcctgtggc ggcagccagc catttggtga cggttttcat gtttgtgtag ttttataggt
     1321 agagatactc cattgtccac ctggaagttg ccaaaaaccg ttccaaaaac gcgttattga
     1381 accgttaacc ggttgtttat tcttgctatt gagatgttaa tcaatcaaaa agggtggttt
     1441 ggcaaaaaat tccggctgga tttggaaatg tcgcgtgcgg gtgactatct ttgcgccagt
     1501 tcttacaaca tctcttatca agaaaggctg agggaaatgg cccggtgaag ccttgacaac
     1561 ctgctgaagc ttggagtcct taaatgggtt ctgctgtttc aagtaaggtg ccaactccat
     1621 cccgatggaa tatcgggaca gataagtcag atcccggctt ggtaatatcg acaaaacata
     1681 ttcaaagctc ttctgacgta tcagaagagt ttttttttgc ccgtccggcc ggcggcggcg
     1741 aaagcggttc ttctgatcat ggccttggta agtggtgtcg ctgaacattt tttaatcatc
     1801 ttaaaatttg tttctcatgc aagcgacaac acaactcatt cacagcattc ctgtcgatcc
     1861 ggcaaccggc gctattgccg taccgatcta ccagacctcc acctttgtgc aggaggctcc
     1921 gggggtaaac aagggctacg attatgcgcg gaccggcaac cccacccgcg ccacgctgga
     1981 aagcctggta gcgcaactgg aggggggcag tacaggcgcc gcttttgcca gcgggctcgc
     2041 ggccatcgat gccgttgtaa agctcctgga ggccggcgat gagatcattg cagtgaatga
     2101 tatttatgga ggggcatacc ggcttttcga gcaggtgtac cggaaattcg ggatcaacac
     2161 gcactatgtc gatacctcca atccggaggc ggtgttccat gcgctgacgc caaagacaaa
     2221 gctcatctgg atcgagaccc cgaccaaccc cacgttgaag atcaccgata tccgggcgat
     2281 cgcaaagatt gcgagagcgc atcgctgcct gctttgcgtg gacaacacct ttgcttcgcc
     2341 cgcgttgcag cagcccttgc tgcttggcgc cgacctcgtg gtccattccg gaactaagta
     2401 cctggggggc catagcgacc tgatcgccgg gctggtggtg acggcggacc ccgagctggg
     2461 gcagaaaata aaattcctgc agaacgccgg cggggccatc ctcgggccgt ttgatagttt
     2521 cctgctgatc aggggcatcg agaccctgca tctgcgcgtc cggcagcatt gcgccagtgc
     2581 gagggcgatc gccgccttcc tggaagatca tccggcagtg aacaaagtgt actatccggg
     2641 gttagtaaag caccccgggc ataccgtcgc agcggggcag tcaaagggtt ttggcggcat
     2701 cgtctcgttc tcgctcaagg aagatacagt agcggcggct acggcctttg tgacctcgac
     2761 gcagctgttc aagctggccg agagtcttgg cgggatcaag agcctggtat cgcatccggc
     2821 ggcgatgacg cacaagacga tcccggcgga gaggcgcaag gctgcgggag tgacggacag
     2881 cctgatccgg ctgtctgcgg ggttggagga taccgaggat ctgatcgccg atctgcggca
     2941 ggctcttgac cggttgacgg tgaaggcgga agtgaccgga tcgcggcaaa aactggaaaa
     3001 agtaatctaa ataaaaaaca agcagtattc atggacacac ataagcagtt gacgatcggt
     3061 ctttttggct ttggagtggt aggagaaggc ctttacaagg tattgaaaca aaccccttcc
     3121 ctgaaggctt ccatcaggaa agtatgtatc aagcatcccg gtaagaagcg ggaagcgccg
     3181 gcggagcttt ttacgacgga ccgggatgcg atcctgaatg atcccgagat caatgtggtg
     3241 gtggaagtga tcaacgagtc ggggccggcc ttcgagatcg tctcgcgggc gttgaagagc
     3301 ggcaagtcgg tggtcagcgc cagcaagaag atgatcgccg agcatctgcc ggagctcctg
     3361 gcattgcaga aggagacgag cgcttccttt ctctatgagg cggcggcctg tgcatcgatc
     3421 ccggtgatcc gcaacctcga ggaatattat gacaacgacc tgttgcattc catcaaggcg
     3481 atcgtcaacg gcagtaccaa ctttatcctg acgaagatgt tcgaagaaaa actggatttc
     3541 caggaggcgc tgctgctggc gcaacagttg ggttttgcgg agagcgaccc gtcgctggat
     3601 gtggaaggct atgatgccgt caacaaatgg acctttctcc tcacgcatgc ctatggtatt
     3661 atcgtggagg cgaaggatct tgtgttcaat ggcatccagc atatacaggc cggggatgcg
     3721 gaggtagcgc gggagaaacg ctatgatatc aagctggttg cccaggccaa gaagctggcc
     3781 aacggcaagg tcgccgcttt tgtgctgccg cagttcatcc gccaggacga tcatcttgcc
     3841 tttgtgaaga atgagttcaa cggggtggtg atcgagagtg ggtttgcaga caagcagttc
     3901 ttttatggca agggggcggg ctccttccct accgcttcgg ccgtgctcag cgatctttcg
     3961 gccctgcggt acgaatacaa atacgaatac aagaaactct atcatcatac gccgcatgaa
     4021 ttgacggatg atttttttgt acgggtcttc ctcagttttg atgatctgaa atatatcccc
     4081 aaggacaggt tccagtccat cgaggagtgg catgccgaca acgatcgaaa atacctcgtg
     4141 ggcgtgctcc ctttccggga gttgaagggt cacagctggt ggcgggagaa tggtacgtcg
     4201 ctggtgctga cgcctgac
//
LOCUS       DATGFG010001071         5751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1314773, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001071 DATGFG010000000
VERSION     DATGFG010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314773"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VKQ52_20310"
     CDS             <1..641
                     /locus_tag="VKQ52_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89614.1"
                     /translation="GDAGIDAGGLQLNTAFGRSWRLQAGAWYQTGHDRDGLTLDAYMT
                     TLALTYQPHLVGATIGWDYLSGNNAFSTSATNHRFDPLYGTPHKFWGYMDYFYAGTGS
                     ATGGLNNPFLKGKYASRNSRLSVELCYHYFGLAANQKDTKGQAVTKRLGSEVDAIVNY
                     GLNKVTTLELGACALYGTHSMEYAKGITPNTARLNASWVYLQLNIRPDFLQK"
     gene            653..1948
                     /locus_tag="VKQ52_20315"
     CDS             653..1948
                     /locus_tag="VKQ52_20315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ89615.1"
                     /translation="MQKINKPLDKIRLFSLDTIQMRTFHITWLMFFVCFFGWFGLAPL
                     MPAIRADLGLTKAQVGNTIIASVSSTIIARLLIGKLCDVLGPRKTAVRLLLIGSIPVL
                     LVGLAKDYTTFLLFRLAIGIIGASFVVTQFHSSMMFASKIRGTANAITGGWGNLGGGV
                     TNMVMPLIFSAIVGFGYTAHQAWRYAMIVPAVMMWIVAFLYYRYTKDTPSGNYTEIGR
                     GPAVRSRTDWSVLADWRVAALTLAYAMCFGMEITFDNVAALHFVDNFHLSQHSAGFWA
                     GLFGFMNIFARALGGIFSDKVGSRWGMRGKGTLLATVLLLEGLGLIAFAHASTFVIAI
                     AFMLSFALFLKMANGAVYGIVPFVNENNIGLISGVVGAGGNLGGMIFGFLFRSESITY
                     AEAFTYIGLTVVTIACIVMVTRWRSTATEGAASTTPATA"
     gene            2005..5517
                     /locus_tag="VKQ52_20320"
     CDS             2005..5517
                     /locus_tag="VKQ52_20320"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLZ89616.1"
                     /translation="MAAVPHRHTERFFSTCCYCGVGCGVTVYKDRKGRITVEGSPEYP
                     VNKGMLCSKGLNLHYTVNDRQDRLLYPQMRQHRGMPLQRVSWDSALDRAAAVFRSLID
                     RYGPDSVGFYISGQCLTEEYYVVNKLMKGFIGSNNIDSNSRLCMSTAVAAHKLSLGED
                     SVPVSYADIELADCFFVTGANPAWCHPILWRRVEAHKTAHPDVKIIIVDPRRTDTAAI
                     ADLHLPLNPGTDTTLHYAIGRLLIEQGDMDPAFIREHTDGFEDYRQRVFARTTAEAAA
                     VCGLPEADIRQAAAWIGAAKGHLSLWTMGLNQSATGVNKVLSLIDLHLLTGKIGIPGS
                     GPFSLTGQPNAMGGRETGGLANLLPAHRDLANPEHRRAVQEYWGSGPLSPKPGLTATE
                     QFQALADGRMKAIWIMGTNPLVSLPDVRLAEAALKKARFVVVQEVSQRPETLAYADLI
                     LPAAAWTEKQGTMTNSERRISLLDKIVDPPGEALPDSVIFSRLAQKMGFPGFDYSSPA
                     EIFREHALLTAGTSIDISGLDYSILQTRRSVQWPYPAGHQETAPGGTERLFTDHHFHT
                     PSGKAVFHAAADELRSEIPDADYPLILTTGRIRDQWHTMTKTGKVNKLRQHIPQAFLE
                     MHPENARALDISEGDIALIESRRGEVRTPVRISDNVRMGTVFLPMHWGKILNSDLNRT
                     NNLTNNLLDPVSKEPDFKFCAVRVGRYKKKKERIVIVGAGAGSHGFVRSYRALNSEDE
                     IIVFNKEDQPFYNRVELPDYISGAKHWDHLVRMTDEEEGDVDILLHRGVSIEKIDRAA
                     RTVTDSTGRVTAYDTLILATGSRAALPKQVPVLEGIFTLRSRVDADRFRKTIPAGGRV
                     VVVGGGLLGLELAASLREMDIAVSIVHRSSRFLDRQLDALASQLLQDEIVDQGCTIYY
                     SDEVQLFYGQTKLTGIRLRSGRVLDCDALVFAIGTVPNIEIAKAAGLDHRRGLIVDGY
                     LRTSDPHIHAIGEIAEFEGNLYGFTAAAEQQAEALARFLNGDIASYYSGTVPMNIIKI
                     HGFDLCSIGLPDCPNTREYEEIIFIDKGRRYYKKCIIHQDRLVGAILVGDKDEFREFR
                     EWITGKIELSDRRLQLLRSGSKAAPVQGKLVCSCNNVGKGNLEQKIDEGCKDVATLCS
                     ATGAGTGCGSCRPEIKRILEAYVS"
     gene            5507..>5751
                     /locus_tag="VKQ52_20325"
     CDS             5507..>5751
                     /locus_tag="VKQ52_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89617.1"
                     /translation="MFRKNYTVKINLPGGIVGAGDLYAIVEAAGRARVEEMQLGTRQQ
                     LFCKVADKYGADLVKELERAGIFYETEKEAYPNIVSS"
BASE COUNT         1169 a         1768 c         1709 g         1105 t
ORIGIN      
        1 ccggtgacgc gggtattgac gccggcggac tgcaattgaa caccgctttc ggcaggtcct
       61 ggcggctgca ggcgggcgcc tggtaccaga ccggccacga ccgcgacggg ctcacgctgg
      121 atgcctatat gacgacgctc gcgctgacct accagccaca cctcgtcggc gccaccatcg
      181 gctgggacta cctctcgggc aacaacgcct tctccacttc cgctacgaac catcgcttcg
      241 accccctcta tggtacaccc cataagttct ggggctatat ggactatttc tacgccggca
      301 ccggttcggc caccggcgga ctgaacaatc ctttcctgaa agggaaatat gcctcgcgca
      361 acagccggtt gtcggtagag ctctgctatc actatttcgg gctggccgcc aaccagaagg
      421 atacgaaagg gcaggcagtt accaaacgcc tgggcagcga agtggatgcg atcgtcaact
      481 acggcctgaa caaggtcacg acgctggaac tgggcgcctg cgcgctctat gggacccaca
      541 gtatggaata cgcgaagggc atcaccccca acacggcccg gctgaatgcc tcctgggtct
      601 atctgcaact gaacatcaga cctgatttcc tccaaaaata aaacctgctg acatgcaaaa
      661 gatcaataaa ccattggaca agatacggct tttctcactg gatacgatcc agatgcggac
      721 ctttcacatc acctggctca tgttctttgt ctgcttcttc ggctggtttg gcctggcgcc
      781 gctgatgccg gccatccggg cggatctggg actgacgaag gcgcaggtgg gcaatacgat
      841 catcgcctcc gtctcgtcga ccatcatcgc gcggttgctg atcgggaaat tatgtgacgt
      901 gctcggtccc cgtaaaacgg ccgtccggct attgctgatc ggttcgatcc ccgtgctgct
      961 ggtgggactg gcaaaggatt ataccacctt tctcctgttc cgcctcgcca tcgggatcat
     1021 cggtgcaagt ttcgtggtca cccaattcca tagttcgatg atgttcgcct cgaagatccg
     1081 gggtacggcc aacgcgatca cgggcggctg gggtaatctc ggcggcggcg tgaccaatat
     1141 ggtgatgccc cttatcttct ccgccatcgt cgggtttggc tatacggcgc accaggcctg
     1201 gcgctatgcg atgatcgttc ctgccgtcat gatgtggatc gtagcctttc tttattatcg
     1261 ttatacaaag gatacccctt cgggaaacta tacggagatc ggccgggggc ctgccgttcg
     1321 cagtcgtacc gactggtccg ttctcgccga ctggcgggta gccgccctga ccctggccta
     1381 tgccatgtgt ttcggaatgg agatcacctt cgacaatgtc gctgccctgc attttgtcga
     1441 caacttccat ctctcccagc atagcgcggg tttctgggcc ggtctctttg gctttatgaa
     1501 catcttcgcc cgcgcgcttg gcggcatctt ctccgataag gtgggcagcc gctgggggat
     1561 gcgcggaaag ggcaccctgc tggcgacggt gctgctcctc gaaggcctgg gactcatcgc
     1621 ctttgcccac gcttccacct tcgttatcgc catcgccttt atgctctctt ttgcactctt
     1681 cctgaaaatg gccaatggcg cggtgtacgg tatcgtaccg tttgtcaacg aaaacaatat
     1741 cgggctgatc agcggggttg taggcgccgg cggtaatctc ggcggcatga tcttcgggtt
     1801 ccttttccgg agcgagtcga tcacgtatgc ggaagccttc acctatatcg ggctgacggt
     1861 agtcaccatc gcctgtatcg ttatggtcac ccgctggcgg tcgaccgcaa ccgagggcgc
     1921 cgcttcaaca acgcccgcaa cggcttaatt cacatcagcc ggagcctgat ccgcagacga
     1981 cgctccggcc ggaattcaat ttatatggct gctgtaccac accgtcacac cgaacgattc
     2041 ttttccacct gctgctattg cggcgtaggc tgcggcgtca ccgtatacaa ggacaggaag
     2101 ggacggataa cggtggaggg tagcccggaa tacccggtga acaagggtat gttgtgcagc
     2161 aagggcctca acctgcacta tacggtcaac gaccggcagg accgcctcct gtatccgcag
     2221 atgcggcagc atcggggcat gcccctgcaa cgcgtgagct gggactcggc cctggaccgg
     2281 gccgcggcgg tgttccggag cctgatcgac cggtatggcc cggatagcgt cggcttctat
     2341 atctcgggcc agtgtcttac cgaggagtac tatgtcgtca ataagttgat gaaggggttt
     2401 atcggcagca acaatatcga cagcaattcc cggctttgca tgagcacggc ggtcgccgcc
     2461 cacaagcttt cccttggcga ggacagtgtg ccggtgagct atgccgatat cgaactggcg
     2521 gattgtttct ttgtcaccgg cgccaacccc gcctggtgtc acccgatcct ttggcgccgg
     2581 gtggaagcgc acaagacggc ccatcccgat gtgaagatca tcatcgtcga tccgcggcgg
     2641 acggacacgg ccgccatcgc cgacctgcac ctgccgttga atcccggcac cgacaccacc
     2701 cttcactatg ccatcggccg gttgctgatc gaacagggcg atatggaccc cgcctttatc
     2761 cgggaacata ccgacggctt tgaggactac cggcaacggg tattcgcccg gacgaccgcg
     2821 gaggcggccg ctgtctgcgg tctgcccgag gccgatatcc ggcaggctgc cgcctggatc
     2881 ggcgccgcga aaggccatct gagtctctgg accatgggac tcaaccaaag cgccaccggg
     2941 gtgaacaagg ttctatcgct gatcgacctt cacctgctga cgggaaagat cgggataccc
     3001 ggctcgggac ccttttccct gacggggcag cccaatgcca tgggcggccg cgagacgggc
     3061 ggcctggcca acctcctgcc ggcccatcgc gatctcgcca accccgaaca ccgccgggcg
     3121 gtgcaggaat actggggctc cggccccctg tcgccaaaac cggggctcac cgcgacggag
     3181 cagttccagg ccctggccga cggccggatg aaggccatct ggatcatggg caccaacccg
     3241 ctggtcagcc tgccggacgt ccgcctggcg gaggcggcgc tgaaaaaggc gcgtttcgtg
     3301 gtcgtacagg aggtcagcca gcggccggag acccttgcct atgccgacct gatcctgccg
     3361 gcggcggcct ggacggagaa gcaggggacg atgaccaact ccgaacgccg gatcagcctg
     3421 ctggataaga tcgtcgaccc gcccggcgaa gccctcccgg actccgtcat cttcagccgg
     3481 ctggcgcaaa agatgggctt ccccggtttc gattactcca gcccggccga gatcttccgc
     3541 gaacacgcgc tgcttacagc cggcacctcc atcgatatca gcggactcga ttactccatc
     3601 ctgcagacac ggcgatcggt acagtggccc tacccggccg gacatcagga gaccgcaccc
     3661 ggcggcacag aaagattatt taccgatcac catttccata ccccatcggg gaaggccgtt
     3721 tttcatgccg cggccgacga gctgcgcagc gaaatacccg acgcggacta tcccctgatc
     3781 ctgaccacgg gccggatccg cgaccagtgg cataccatga cgaagacggg caaggtcaac
     3841 aaactccggc agcatatccc ccaggccttc ctggagatgc acccggagaa cgcccgggcg
     3901 ctggatatca gcgaaggcga tatcgccctt atcgaatccc ggcgcggcga ggtaaggacg
     3961 ccggtgcgca tatccgataa cgtaagaatg gggaccgtct tcctccccat gcactggggg
     4021 aagatcctga acagcgatct caaccggacc aataacctga ccaataacct gctcgacccg
     4081 gtgtccaagg agccggactt caagttctgc gcggtgcgtg tcgggcgata caagaaaaaa
     4141 aaggagcgca tcgtcatcgt cggcgcgggc gccgggtcgc atggctttgt acggagctac
     4201 cgcgcactca attcggaaga cgagatcatc gttttcaaca aggaggacca gcctttttac
     4261 aacagggtgg agctcccgga ctatatcagc ggcgcaaagc actgggacca cctcgtccgc
     4321 atgaccgatg aggaggaagg cgatgtcgat atcctgctgc accgcggtgt cagcatcgaa
     4381 aagatcgatc gcgccgccag gacggtgacg gactccaccg gcagggtcac cgcatacgac
     4441 acgctgatcc tcgctacggg gagccgggcg gccctgccca aacaggtgcc ggtgctggaa
     4501 gggatattca cccttcgcag ccgggtggat gccgaccgtt tccggaaaac catcccggcc
     4561 ggcggccggg tagtggtcgt cggcggcggg ctgctgggcc tggaactggc cgcctccctg
     4621 cgggagatgg atattgccgt ctccatcgtc caccggagca gccgctttct cgaccggcag
     4681 ctggatgccc tcgccagcca gctcctgcag gacgagatcg tcgatcaggg ctgcaccatc
     4741 tactattccg acgaagtaca gctcttctac ggccagacaa aactcacggg tatccggctt
     4801 cgaagcggtc gcgtcctgga ctgtgacgcc ctcgtattcg cgatcggcac ggtacccaat
     4861 atcgagatcg caaaggctgc cggtctcgat caccgccgcg gcctgatcgt cgacggctat
     4921 ctgcgtacca gcgatcccca tatccatgcc atcggggaga tcgcggaatt cgaaggcaat
     4981 ctctatggat ttacggccgc ggccgaacag caggccgagg cgctggcacg gttcctgaac
     5041 ggggatatcg ccagctatta cagcgggaca gtgccgatga acatcatcaa gatccacggc
     5101 ttcgaccttt gcagtatcgg gctgcccgac tgtcccaata cccgggaata cgaagagatc
     5161 atctttatcg ataagggacg ccggtattat aagaaatgta tcatccacca ggaccggctg
     5221 gtgggcgcca tcctggtcgg cgacaaggat gagttccggg agttccggga atggatcacg
     5281 gggaagatag agttgagcga ccggcggctg caattgctgc gtagcggcag caaggccgcg
     5341 cccgtacagg gaaaactggt ctgtagctgc aacaacgtcg gcaaaggcaa cctggaacag
     5401 aagatcgatg aaggatgtaa ggatgtggcg accctgtgtt cggccaccgg cgcgggtacc
     5461 ggttgcggaa gctgccgccc ggaaataaaa aggatattgg aagcctatgt ttcgtaagaa
     5521 ctataccgtt aagatcaacc ttcccggcgg catcgtcggc gcgggtgatc tgtatgccat
     5581 cgtggaggcc gcgggccggg ccagggtaga agagatgcaa ctgggcaccc ggcagcagct
     5641 gttctgcaag gtggccgata agtacggggc cgatcttgta aaggaactgg agcgggccgg
     5701 catcttctat gaaacggaaa aagaggccta tccgaatatc gtgagctcgt a
//
LOCUS       DATGFG010001072         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_175048, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001072 DATGFG010000000
VERSION     DATGFG010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175048"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VKQ52_20330"
     CDS             <1..342
                     /locus_tag="VKQ52_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89618.1"
                     /translation="VCLDIVFESMRRLKAAGDEWLKDCWLWLYKGAWQEWDISEIEMA
                     IPMSPDQMLKKRFGIFIHQSQKDMVPFQGSDSREFWQRAEDRNANTANLYAQLGLTKY
                     AAMEAFVRWHY"
     gene            451..1089
                     /locus_tag="VKQ52_20335"
     CDS             451..1089
                     /locus_tag="VKQ52_20335"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HLZ89619.1"
                     /translation="MPQTTFIEVRQEWQEAWLVTAFRRRVMTGLVILAAMLSILPRFF
                     QAIERRHGIVLNDPVLRMLPPHNVSMPLFIVIWAFSAYCIYRAAQMPRMFLTFLWSFI
                     FLCAFRMLTITLVPLDPPAGLIGLVDPLSNFFYGGDKFVTKDLFFSGHTSAVFLLYLT
                     VPGKTDKKLGLVVTAVVGFLLLVQHVHYTLDVLGGLVFCWLSYQIATRTVLR"
     gene            complement(1144..1935)
                     /locus_tag="VKQ52_20340"
     CDS             complement(1144..1935)
                     /locus_tag="VKQ52_20340"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLZ89620.1"
                     /translation="MNEQLSYSSFPDNFWGLGKRAPDTSKESYKFKQYYIYLHPQCLI
                     FKSLYLGTLYEYQRVFDITYPDGGLFDRQNVPGRFGYHVSGLGLSFTYDTRNNAFSPD
                     KGVMLQFYFDHFSPLFGSDYQYTNYVVDLRRFLRIYKQQVLAIQAFGSFNSGEVPLRS
                     LAYLGGANTMRGYYAGRYRDKNAAVIQAEYRVPLFWRIGAVGFGDIGNVGPEVKDIDF
                     HHFKYSYGGGLRVALNQKEKLNLRLDYGIAKGSSQGFYLQIGEAF"
     gene            2484..3299
                     /locus_tag="VKQ52_20345"
     CDS             2484..3299
                     /locus_tag="VKQ52_20345"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLZ89621.1"
                     /translation="MKKFECPDFVLGDKLTEEQFNFFDKYGVIIFRNFLNSEIVQLFI
                     LELNRIEKQWLDEGREKVNGIPLKFGKDQDGNKMIQRMCFLSLYSEPLHEMLQDPRIK
                     ALVGLLYPYEGRLSENEKDGLILNHYVRMPESNFTQMGWHTDSPRDLFLGQKIMPMLN
                     VGIHLDDTPFENGGLRVLPGTHKQGMLRLLFGKKYFIDNNPDRREIGFDLFAGDLTVH
                     DGRIWHRAQQSPLYGEASRRRVMYVPIITGKYMPKDANSKTPFYHRLASKVQN"
     gene            3403..>3940
                     /locus_tag="VKQ52_20350"
     CDS             3403..>3940
                     /locus_tag="VKQ52_20350"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ89622.1"
                     /translation="MMEYALITGASKGIGRAIARELAARGYNVLLVARSEDLLQQLAT
                     EIGSRHKVKTDFLALDLSMTEAPQTVYDWCRARGYDVSILVNNAGYGLSGPFEKYSLQ
                     EHLNMMQLNMSTLVGMTRLFLPDLHRRKKGYILNIASSAAYQAVPKLSLYAATKSFVL
                     AFSRGLHQELHKSTVSVTC"
BASE COUNT          902 a          963 c         1099 g          976 t
ORIGIN      
        1 gtttgtctcg acatcgtctt tgaatccatg cgccggttga aggctgccgg cgatgagtgg
       61 ttgaaggact gctggctctg gctgtacaag ggtgcatggc aggagtggga tatcagcgag
      121 atcgagatgg ctatacccat gagccccgac cagatgctga agaagcgctt tggcatcttc
      181 atccaccagt cgcagaagga tatggttccc ttccagggta gcgatagccg tgaattctgg
      241 cagcgcgcgg aggaccgcaa cgccaataca gcgaatctat atgcgcagtt ggggttaacc
      301 aagtatgctg ctatggaggc gttcgttaga tggcactatt agacggcctc cggccgtttc
      361 cgtctcgcat tgcggcccgg ttggtccaca atgttataat cgcattaaat ttgcgttaac
      421 taattctgga ttgtattttt gtggcttcac atgccacaaa caaccttcat tgaagtaagg
      481 caggaatggc aggaagcctg gcttgtcacc gcttttcgcc gtagggtgat gaccggcctc
      541 gtcattcttg ccgccatgct gtcgattctt ccccggttct tccaggcgat cgagcgtcgg
      601 catgggatcg tgttgaacga tcctgtgctg aggatgctgc cgccccacaa tgtatcgatg
      661 ccccttttta tcgtgatctg ggctttttcc gcgtattgta tctatcgtgc cgcgcaaatg
      721 ccccggatgt tcctcacctt tctgtggtcg ttcatcttcc tttgcgcctt tcgtatgctg
      781 acgataacgc tggtgccgct ggacccgccg gcagggttga tcgggcttgt cgatccgctg
      841 agtaatttct tttatggcgg ggataagttc gttacgaagg atctcttctt ctccggtcac
      901 acctctgcgg ttttcctgct ctatctcacc gttcccggaa agaccgacaa gaagctggga
      961 ctcgtggtga cggcggtcgt tggctttctg ctgctggtgc agcatgtcca ttataccctc
     1021 gatgtgctgg ggggattggt cttctgctgg ctttcctacc agatcgctac ccggacggta
     1081 ttgcggtaag gggtacgatg tgctatgccg gcatctcatc cgcctcgccg gagtgcatcc
     1141 gccctagaac gcctcgccga tctgcaaata gaatccctgt gaggatcctt tggcgatgcc
     1201 ataatcgagc cggaggttga gtttctcctt ttgattcagt gctacccgaa gaccaccgcc
     1261 ataggagtat ttaaaatggt ggaaatcgat gtcttttact tccgggccga cattgccgat
     1321 gtcgccgaaa ccgacggcgc cgatccgcca gaagagggga acacggtatt ccgcctggat
     1381 aacggcggca ttcttgtccc ggtagcgccc ggcataatac ccgcgcatcg tattggcgcc
     1441 gccgagatag gccaggctgc gcaggggcac ctcgccggaa ttgaagctgc cgaacgcctg
     1501 gatggccagc acctgctgtt tgtagatgcg gaggaagcgg cggaggtcca caacgtagtt
     1561 agtatattga taatcagagc caaataaggg tgaaaagtga tcgaaataga actggagcat
     1621 taccccttta tccggtgaaa aggcattgtt ccgggtatca taggtgaagc tgaggccaag
     1681 gccggataca tgatagccaa agcggccggg gacattttgc cggtcgaaca ggccgccatc
     1741 gggataggtg atgtcgaaaa cccgctggta ttcgtagagg gtgcccagat aaagactctt
     1801 gaagatcagg cattgcgggt gcaggtatat ataatactgt ttaaatttat acgattcctt
     1861 gctggtgtcg ggggctcgtt tgccaaggcc ccagaaatta tcgggaaaag agctatagga
     1921 cagttgttcg ttcaaaatga aacgttcgcc ggggaagtaa atggaaccat ttaaagccac
     1981 tacaaactgc cggcgggtcg tataaagcgc taaggcctgg gcgttagagg ttcgtatagc
     2041 cgtgtcacgg cttttgaggt gaaaagtgga ggcgctggcg agaccgaaag accatcccgt
     2101 ctctatggat cgggcaatga ttggcagtac gagaataggg tgtatatttg ccgggctttt
     2161 tatcgttaac ggcagctttt caacagggac tgttttgagg ctgtcatttt gggcttctgc
     2221 catatggggc aaaagggcaa ggcagaaaag cccaagaaaa tagataaata ttgcttttcc
     2281 gaacgaataa gagagcaatg ttgtagtata tttatttacg tttgtcgata aaaaggaggc
     2341 aaattaacgc ggaaaatcta attttcttac atttgatgtt taatgaagat ttcacaattt
     2401 gttaaaattc aattaaccca atattttgaa gctctgaagc ggaaagggat gacccttcct
     2461 tttctaaaat aaagctagca tttatgaaaa aattcgaatg ccccgatttt gtcttgggag
     2521 ataagctaac ggaagaacaa tttaactttt tcgacaagta tggcgtgatc atttttagga
     2581 attttctgaa ttcggagatc gttcaattat tcatactaga gcttaacagg attgaaaaac
     2641 aatggctgga tgaaggccgg gaaaaggtca atggcatccc gttgaaattt ggcaaggatc
     2701 aggacgggaa caagatgatc caacggatgt gtttcctgtc cctgtacagc gagccgctgc
     2761 atgagatgct gcaggatcca cgcatcaagg cgcttgtggg attactgtat ccatacgagg
     2821 gccggctcag cgagaatgag aaggatggat tgatcctcaa tcactacgta cggatgccgg
     2881 aaagcaattt tacccagatg ggctggcata cggacagccc ccgggacctg ttcctggggc
     2941 agaagatcat gcccatgctg aacgtgggta tccacctgga cgacacccca ttcgagaatg
     3001 gcgggttgcg ggtattgccg ggtacgcata aacaggggat gttgcggctg ttgttcggta
     3061 agaagtattt catcgacaat aaccccgatc gccgggagat cggcttcgat ctgtttgccg
     3121 gggatcttac cgttcatgac ggccggatct ggcatcgcgc tcagcagtcc ccgctctatg
     3181 gggaagccag ccggcgccgg gtgatgtatg taccgatcat taccgggaaa tatatgccga
     3241 aggatgcgaa cagcaagacg ccgttctacc atcgccttgc ctcgaaagtg cagaattaac
     3301 gatatttact tgcccaggcg gcccgttccg atgaggatca ggccgtcttt ttttgcccct
     3361 gcggaatcgc ctcgtttcag gcggttcgcc tatctttggc gcatgatgga atatgcactt
     3421 attaccggag ccagcaaggg tattggccgg gcgatcgccc gcgagctggc ggcccgggga
     3481 tacaatgtcc tgcttgtcgc ccgttcggaa gacctgctgc agcaactggc caccgagatc
     3541 ggctcccgcc acaaagtaaa gaccgatttc ctcgccctcg atctctcgat gaccgaagcc
     3601 cctcaaactg tctatgactg gtgccgggcc agggggtatg acgtcagcat tctcgtcaac
     3661 aatgccggtt atggcctcag cgggcccttc gagaagtaca gcctgcagga acatttgaat
     3721 atgatgcagt tgaatatgtc gacgctggtg gggatgacgc ggctcttcct gcccgatctc
     3781 catcgccgga agaagggcta tattctcaat atcgccagct ccgccgccta ccaggccgtg
     3841 ccgaagctga gtctctatgc ggctacgaag tcctttgtac tggcctttag ccgcgggctg
     3901 caccaggaac tgcacaagag taccgtttcc gtcacctgtg
//
LOCUS       DATGFG010001073         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1660366, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001073 DATGFG010000000
VERSION     DATGFG010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660366"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VKQ52_20355"
     CDS             complement(<1..452)
                     /locus_tag="VKQ52_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89623.1"
                     /translation="MKQAALILAAILCFAACKTSVRDSGCITRYLGTGATSYLKPGQL
                     DTIRQLFRTNNISTAGLEFTYYSSDTATDSGVFLLNQGVSAVPTINSLPVFFVSDYWY
                     FRNGSFFPRYSGVVDYSYPGSDTSGHQPLAALRSLFFKEYDSVIYSSSA"
     gene            613..1185
                     /gene="nudK"
                     /locus_tag="VKQ52_20360"
     CDS             613..1185
                     /gene="nudK"
                     /locus_tag="VKQ52_20360"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HLZ89624.1"
                     /translation="MKNIEITNEQILSDNWYVLRKFTYRFQQHGEWVERQREAYDRGN
                     GAAILLYNRQQGTVILTRQFRLPTFVNGNASGMLIEACAGLLDRDHPEVAIRRETQEE
                     TGYQLGEVEKVFEAYMSPGSVTELLYFFIAEYIPSMKVDEGGGLEHEEEDIEVLELPF
                     ARALAMIDSGEIRDGKTIMLLQHVRLKGVL"
     gene            1198..2766
                     /locus_tag="VKQ52_20365"
     CDS             1198..2766
                     /locus_tag="VKQ52_20365"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLZ89625.1"
                     /translation="MRKLLSMLLWLGVVTAVRAQPLSSVAIDSLVERTLRTFDVPGIA
                     VCVIKDGKVIHSKGYGVRSLATRQPVDENTLFGIASNSKAFTTAALGMLVDEGKLHWD
                     DRVRKYIPEFKLYDPYVTEEFTIRDLLCHRSGLGLGAGDLMFFPDSSDFTIPDILHNL
                     QFLKPVTSFRSTYAYDNNLYIVAGEVVARVSGMSWEDFIEKRIMAPIGMTHSAASWDR
                     LRDTTDWIDGHARVEGVVRVIARSRSGVDHAAGGIYSSIADLSKWVQLHLAGGKGLFS
                     PAVLRERWAPQTILPIGGPGPYNTHFAAYGLGFGVSDVKGYKQVSHTGGLEGMVTQIT
                     MIPELQLGIIVLTNQEEGLAFSAVTNQIKDGYLGVPGTDRVAEYAGIRKRQLADEKRV
                     VDSIWNEVAAAQAARAPAGRKEAAGGGVKGVDMAIYAGTYRDVWFGEVVISVKNGKPW
                     FDSKRSPKLTGELLPYKGNTFIARWKDRSMGADAYVVFELDERGIGSGIKMKPVSPLT
                     DFSYDFQDLDLRRE"
     gene            2778..>3012
                     /locus_tag="VKQ52_20370"
     CDS             2778..>3012
                     /locus_tag="VKQ52_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89626.1"
                     /translation="MVKYFLIAAALMGGLRAGAQGNRGAQDYRVVHAKAIVVDTHNDV
                     LSSATIGQGLDFGMDQTGKTHSDLGRWKKGGVDV"
BASE COUNT          604 a          592 c         1145 g          671 t
ORIGIN      
        1 gcggaggatg aatagatgac cgagtcatat tccttgaaaa agaggctgcg cagggcagcg
       61 agcggctggt ggcccgaggt atcgctgccg ggatacgaat agtcgacaac cccgctatac
      121 cttggaaaga agctgccgtt gcggaagtac cagtagtccg acacgaagaa gacgggcagg
      181 ctgttgatgg tggggacggc cgatactccc tggttgagga ggaatacgcc ggagtcggtg
      241 gcggtgtcgc tggagtagta ggtgaattcg aggccggcgg tagagatatt gttggtcctg
      301 aagagttgcc tgatggtgtc gagctggccg ggtttgaggt aggatgtggc tccggtgccg
      361 aggtagcggg tgatgcagcc gctgtcgcgg acgcttgttt tgcaggcggc gaagcagagg
      421 atggcggcca ggataagggc ggcttgtttc attgcgggtg tttcgcctat gacaagggaa
      481 ttggcccaac cgttgcattg ccgtacaagg gtgcgacgca acggatgctg agaagagata
      541 tggcgccggc ctccgcccga agaccgatgt cgaaattatc gatatcttcc atttcttcaa
      601 tatcttcggc tgatgaagaa tatcgagata acgaatgagc agattctttc ggataattgg
      661 tatgtgttgc ggaagtttac ctatcggttt cagcagcatg gggaatgggt ggagcggcag
      721 cgggaggcgt atgaccgggg gaacggggcg gcgatcctgt tgtataaccg gcagcagggt
      781 acggtgatcc tgacgcggca gttcaggctg ccgacgtttg tgaacgggaa tgcgtcgggg
      841 atgcttatcg aggcttgtgc ggggttgttg gacagggatc acccggaggt ggcgatccgg
      901 cgggagacgc aggaggagac ggggtatcaa ttaggggagg tggagaaggt gttcgaggcg
      961 tatatgtcgc cggggtcggt tacggagctg ttgtattttt ttatcgcgga gtatatccct
     1021 tcgatgaagg tggatgaggg tggggggctg gaacatgagg aggaggatat cgaggtgttg
     1081 gaactgccgt ttgcgcgcgc gctggcgatg atcgatagtg gggagatcag ggatgggaag
     1141 acgatcatgc tcttgcagca tgtgcggcta aagggtgtgc tataaatttc gactgatatg
     1201 agaaaacttt tgtcaatgct gctctggctt ggcgtggtta ccgccgtgcg ggcgcagcct
     1261 ttatcttccg ttgctatcga cagcctggtg gagaggacgt tgcgcacctt cgatgtgccg
     1321 gggatcgccg tttgtgtgat caaggatggg aaggtgatcc actcgaaggg gtatggggtg
     1381 cggtcgctgg ccacgcgtca gccggtggat gagaatacgc tgtttgggat cgcgtccaac
     1441 agcaaggcgt tcaccaccgc ggcgctcggg atgctggtgg atgaggggaa gctgcattgg
     1501 gatgaccggg ttcggaagta tatacccgag ttcaagttgt acgatccgta tgtcaccgag
     1561 gaatttacga tccgggacct gctttgtcac cggagcgggt tggggttggg cgccggtgat
     1621 ctgatgttct ttcccgactc gagtgatttt acgatcccgg atattctgca taatctgcaa
     1681 tttttgaagc cggtgaccag tttccgatcg acgtatgctt atgataataa tctgtatatc
     1741 gtggcggggg aggttgttgc gcgggtgagt gggatgagct gggaggattt tatcgagaag
     1801 cggatcatgg cgccgatcgg gatgacgcat agtgcggcca gttgggaccg gctgagggat
     1861 acgacggatt ggatcgacgg gcatgcgcgg gtggagggtg tggtgcgggt gatcgcgcgg
     1921 agccggagcg gggtggatca tgcggcgggt ggtatctatt cgagtattgc cgatctgagc
     1981 aagtgggtgc agttgcacct ggccggcggg aagggacttt tttcaccggc ggtgttgcgg
     2041 gagcgctggg cgccgcagac cattttgcct atcggcgggc cggggcctta taacacgcat
     2101 tttgcggcgt atggccttgg gttcggggtg tcggatgtga agggctataa gcaggtgagc
     2161 cataccggtg gattggaagg gatggtgacg cagatcacga tgatcccgga attgcagctg
     2221 gggatcattg tgctgacgaa ccaggaggaa gggcttgcgt tcagtgcggt caccaaccag
     2281 ataaaggatg gttacctggg ggtgccgggt acggacaggg tggcggagta tgcggggata
     2341 cggaaaaggc agttggcgga tgagaagcgg gttgtcgatt cgatatggaa tgaggtggcg
     2401 gcggcgcagg cggcccgggc gccggcgggg aggaaagagg cggccggcgg gggtgtaaaa
     2461 ggggtggata tggccattta tgcgggcacg taccgggatg tgtggtttgg ggaggtggtg
     2521 atctcggtga agaacgggaa gccctggttc gattcgaagc ggagtccgaa gctgacgggg
     2581 gaattgttgc cctacaaggg gaatacgttt attgcgcggt ggaaggaccg gagtatgggc
     2641 gccgatgcct atgtggtatt cgagttggat gagcggggga tagggagtgg aataaagatg
     2701 aagccggtgt cgccgctgac ggatttcagt tatgatttcc aggacctgga tctgagaaga
     2761 gaataaatca attttttatg gtaaagtatt ttttgatcgc ggcggccctg atgggcgggc
     2821 tgcgggccgg cgcccagggg aatcgcggtg cgcaggatta tcgggtagtg catgcgaagg
     2881 caatcgttgt ggacacgcat aatgatgtgt tgtcttcggc cacgatcggg caggggctcg
     2941 atttcgggat ggaccagacc ggtaagacgc attccgacct ggggcggtgg aagaaggggg
     3001 gtgtggatgt gc
//
LOCUS       DATGFG010001074         8489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1309038, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001074 DATGFG010000000
VERSION     DATGFG010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309038"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VKQ52_20375"
     CDS             complement(<1..354)
                     /locus_tag="VKQ52_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89627.1"
                     /translation="MHRLIAVGIFVILAAGCSNRQQAAMDHALTVAGENRDQLQRVLD
                     HYSRPKDSLKLAAAKFLIANMPYHYGYSGAELKKYGEVFTLIDSLNYIRGGLTTADRS
                     RLGDQVVSRYGWPKAD"
     gene            complement(357..2351)
                     /locus_tag="VKQ52_20380"
     CDS             complement(357..2351)
                     /locus_tag="VKQ52_20380"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HLZ89628.1"
                     /translation="MSRLSRRPTGVFLFALFLTACNPAGQPRRGNYYQAALLQCATEY
                     GIRGLSFRLIREGRIWNEYHADDAAGSMPGRSSEIRNTFITPLLLDALEKDSVLRATD
                     SIAPGVTVADLVTTPADRYAVCSDHRGYERLALARMNRPDSKAGVTAFYKNRLSFSGR
                     DNVKDIFDDLQRLSAYMDENFPEFGVSDDSIPDLFPGRYTAGVAFFYGWRVLKFQGNT
                     ILWNCFQQGENTLLVIKSMEKKILFTACYRTQNIPTPFDHHRQVLLQSPLALAFIEAL
                     YIPQMAGFDYAQKATTLAPALGSLPANMQFLACNDLAAHAALYDRRGNSGQAGVLRRV
                     LSGLTGDSLLLRYRDDDVLAAIEYATNDLHAAVSFHLDKDTTLQLFTGGQSRVPVNYN
                     ENDYQYDNIQLFIEGGRQIHLFQFNYGCPLPRRSPVDAFRFGDATDTSYVLEASISRR
                     SLADGRWPFQRLYRANVLLGDCDLYEDYRESVLSWAVHQHQDFSDATKYGKIILAGQA
                     GSMDSTLLYALPSHPAPVIDGKVDKIWDRAPWSLVTRLYMGAPVAPFDLSGRFKVLYD
                     DRSLHFLFEITDDCRNRLGLVAPDKCWIEDASSGRIIWKMDADTTYGCGTFSLRRKIR
                     LPAGNYLLKYMSDGDNSYDHWFGKPPRNGVYGALLYISKP"
     gene            complement(2348..2593)
                     /locus_tag="VKQ52_20385"
     CDS             complement(2348..2593)
                     /locus_tag="VKQ52_20385"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ89629.1"
                     /translation="MPHYNFSRLEYLDVLIRHKSTGAPKALARKLNISLRAVHNYINM
                     LKDQGAPINYNKHKETYYYDEEGYFCFKFIRQEEKNV"
     gene            complement(2778..3521)
                     /locus_tag="VKQ52_20390"
     CDS             complement(2778..3521)
                     /locus_tag="VKQ52_20390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ89630.1"
                     /translation="MLKCIIVEDETLAQDVIKSHLARMDRMELVGVFRTGPEAMEALK
                     AQEVDVIFLDIRLPGMSGLHFLRGLVDPPLVVLTTAYAEYALESYEFNVIDYLLKPIS
                     FERFSKAVNKLLDGRLYPIPGGAAAGRGPGSGLGPEEPALPQGDHIFVKSNSKFFRVN
                     FSEILYVEGMKDYLKIHTPEYTLVTHQTMNELEKLLPSRMFIRVHRSYLVAMGHIRAI
                     YGNSIELGKVTIPIGVNYKEGVMGLIGRR"
     gene            complement(3515..4660)
                     /locus_tag="VKQ52_20395"
     CDS             complement(3515..4660)
                     /locus_tag="VKQ52_20395"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLZ89631.1"
                     /translation="MEYDNDRLRKRRPQVETFYWIMLVLLQPVSCWVLNPFRWLWPFL
                     LAGNLLVFPAYVIYSRLMGSALAIRGRRWFPILLSISAFLVIQAFLLCVYSLFEKMAS
                     APMKLYFSVNPAHLLRDGWWTAVNMAFSMAVFYIRKADDEKDQLVEMQKDNTFFRLRY
                     LRAQLNPHFLFNTLNSIYSLSMQKSDRTAEIVVRLADIMRYLIYECNEDKIPLDKEIE
                     FIRNYIEIEKLRHKADIRLTVEGQTAGLMIEPYLFISFIENGFKHAMDHSFGEPFIYI
                     TLRVGSEQLVLNVINSTDADLELQAKKLNGMGIAHSRSLLELLYPASYDLDIIQTEKD
                     ETRTSALRLRNARERLENLYPDSHTLDVILNKSTFTVSLILKKFAAC"
     gene            4819..5040
                     /locus_tag="VKQ52_20400"
     CDS             4819..5040
                     /locus_tag="VKQ52_20400"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HLZ89632.1"
                     /translation="MATTRITINSNGSLKLEGDFELVDRNGTPYGLGGREIVSLCRCG
                     RSQNKPFCDGSHKGHFEHDATAFDLPPKK"
     gene            5078..5635
                     /locus_tag="VKQ52_20405"
     CDS             5078..5635
                     /locus_tag="VKQ52_20405"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HLZ89633.1"
                     /translation="MIAYQDEQLLLLLKSDNEAAFTEIYHRYWQRLFIVAMHRLANTE
                     EARELVQDIFCNLWKRRHSLQIDYSLNTYLSAAVKYEVINRLAQKGRQQRFRSKLSRE
                     WLEASKDTENQLQFNELQDQLAALVQTLPEKCRIIFQLSREKGYSQKRIAAELGIAEK
                     TVEAHLANALRKIRTGLSHLFFFLF"
     gene            5701..6957
                     /locus_tag="VKQ52_20410"
     CDS             5701..6957
                     /locus_tag="VKQ52_20410"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ89634.1"
                     /translation="MTDLPYSNRILELADKWIKGSITESEKNEFIDWYRRFDDHELLL
                     TPEFKPLIKGLEADMLVNIRRRIAGDESPPIRTTPSEHKGRLVKIKTWQIAAAAILLL
                     TASMLPFLLARKTRRENAVALQKPAPAPPPRTDILPGSNKAILTLANGSTITLDSAHN
                     GNLTQQGNTLVVKSAGGSLRYNLLSKDQGKDQSNQQRSNAVAYNVLSTPRGGQYQLVL
                     PDGSRVWLNAASSIRYPTAFAGANREVEVTGEAYFEIAKNAAMPFRVLTVNHLGDTDP
                     MAVDVLGTDFNVNAYADESVARTTLVEGMVRVSKGNSKTLLQPGQQSELQKNGAITRV
                     ADADVEKAVAWRNGIFEFGDEELPVIMRQISRWYDVDVVYQGPVPTDRFTGRVTRNTS
                     LSGVLKILKLSDVQFTVADNKIIVHS"
     gene            7210..>8489
                     /locus_tag="VKQ52_20415"
     CDS             7210..>8489
                     /locus_tag="VKQ52_20415"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLZ89635.1"
                     /translation="MKITAFLLLAACIHVSATGFSQNISLSVHEAPLTKVFREIQRQS
                     GYSFWYKTRQLDKAVKVTLDLRNATLQEALTKAFENQPLDFVIVEKTVVIRPRDLAAA
                     PAPPPIIIKGRVSTASGKGLGGVSVTVKATKKGTVTDENGEYTISVDKGEVLVFSYVG
                     YDTREVRVGDQTSIAVSLVEANASLNEIAVIGYGRASRKSLASAINTVKSEDLNKGAI
                     TDVGQLLQGKVPGLNITASGDPNAPAAVILRGTSTLNSNQTPFYVIDGVPGADISLIA
                     PDDIQSIDVLKDAAATAIYGNRAAAGVIIVTTRKGKTGQMQVGYSGYVGAEEVSSRLK
                     VMNASQLRAFLSKNNEGFSPTDDKGANTDWQKAIERTGVSHNHNLSFSGGNEHGSYIA
                     SINYADKEGVLKNSDLRRLIARLSVEQYALNDKV"
BASE COUNT         2114 a         2484 c         2117 g         1774 t
ORIGIN      
        1 atcggctttc ggccagccat aacgggatac tacctggtca cccagccggc tccggtctgc
       61 cgtggtaagc ccgccgcgga tatagttcag cgaatcgatg agcgtaaaca cctcgccata
      121 tttctttagc tccgccccgg aataaccata atgatacggc atgttggcga taaggaattt
      181 cgctgccgca agctttaagc tgtcctttgg ccgggaatag tggtcgagca cccgttgcag
      241 ctgatcccgg ttctccccgg cgaccgtcag cgcatggtcc atcgctgcct gttgcctgtt
      301 gctacagccg gcggcaagaa tgacaaaaat gcccacagcg ataagtcggt gcatagtcat
      361 ggttttgaga tatacaatag ggcgccatac accccatttc gcggcggctt tccaaaccag
      421 tgatcgtagg aattgtcccc gtcggacata tatttcagca gatagttgcc ggctggcagt
      481 cttatctttc ggcgcaggct gaaggtgcca cacccatagg tggtatcggc atccatcttc
      541 cagatgatgc ggccgctgga cgcatcctcg atccagcatt tatcgggggc cacaaggccc
      601 agccgattgc ggcagtcatc ggtgatctcg aaaagaaagt gcagcgaacg gtcgtcgtaa
      661 agcaccttga accggcccga caggtcaaac ggggcgacgg gggcacccat gtaaagccgg
      721 gttaccagcg accagggggc gcgatcccag atcttgtcga cttttccatc gatgacaggg
      781 gcgggatggc tgggcaacgc atacagcagc gtgctatcca tacttccggc ttgcccggcc
      841 aaaatgatct ttccgtattt ggtcgcgtcg gaaaaatctt gatgctggtg cacggcccag
      901 gataatacgc tttcccggta gtcttcataa agatcacaat ccccgagcag cacattggcg
      961 cgatagagcc gttggaaggg ccatcggccg tctgccagtg atctccggct gatgctggct
     1021 tcgagaacgt agctggtgtc tgtcgcatcg ccgaaacgaa acgcatccac cgggctcctt
     1081 ctgggtaaag gacagccata attgaactgg aacaggtgta tctgccgccc accttctata
     1141 aataattgga tgttgtcgta ttgatagtca ttttcattgt aattcactgg aacccgggac
     1201 tgcccgcccg tgaaaagttg cagggtagtg tccttgtcga gatggaagga caccgctgca
     1261 tggagatcgt ttgtggcata ttcgatggcc gccagcacgt cgtcgtccct atagcgcaaa
     1321 agcaggctgt cgccggtcag cccggacaga acacggcgga ggacgccggc ttgtcccgag
     1381 ttgccgcggc gatcgtataa agctgcatgg gccgccaggt cgttgcaggc aaggaactgc
     1441 atattcgcag gtaacgatcc cagcgcgggc gccagcgtcg ttgccttttg agcataatcg
     1501 aacccggcca tctgcggaat atacaatgct tcgataaagg caagcgccaa aggcgattgg
     1561 agaaggactt gccgatggtg gtcgaagggt gtggggatgt tctgcgtccg gtagcaggca
     1621 gtgaacaata ttttcttttc catgctcttg atcaccagca aggtattctc cccctgttgg
     1681 aagcaattcc acaagatcgt gttgccttgg aattttaata cccgccatcc gtaaaagaat
     1741 gcaacacccg ccgtatacct tcccgggaac agatcgggta tagagtcatc cgacactccg
     1801 aactccggga aattttcatc catataggcc gacaggcgct ggagatcatc gaatatatcc
     1861 ttcacattgt ctctgccgga aaaggacagt ctgttcttat agaacgccgt cacaccggcc
     1921 ttgctgtccg gccgattcat ccttgctaaa gccagccgtt cgtacccgcg atgatccgaa
     1981 caaacggcat agcgatccgc cggcgtggtg accaggtcgg ctactgtcac ccctggcgca
     2041 atgctgtccg tcgcgcgaag tacagagtcc ttttccaggg catccaaaag cagcggtgtg
     2101 atgaaggtat tgcgaatctc cgacgagcgg ccgggcatgc tgccggcagc atcgtcagca
     2161 tgatattcat tccatatcct gccctcccgg ataagcctga aagacagccc gcggatgcca
     2221 tattccgtcg cacattgcag caatgcagcc tggtaatagt tgccccggcg cggttgcccg
     2281 gcgggattac atgctgtcag gaaaagggcg aacaagaaaa ctccggtagg ccgcctgctg
     2341 agcctgctca tacgtttttc tcttcttgcc ggatgaattt gaagcagaaa tacccttcct
     2401 catcgtaata ataggtctct ttgtgcttgt tgtagtttat cggcgccccc tggtctttca
     2461 acatattgat gtaattatgg acggcgcgga gagagatgtt caatttcctg gccagtgctt
     2521 tgggcgcacc tgtcgattta tggcggatta atacatcgag gtattccagc cgggagaaat
     2581 tgtaatgggg catggcaagg ttagcgataa aggttaatga ttaactgttc tatactatac
     2641 tatactactt gtacgaatat agcgcctttt agcaacatct gcccaagaat tgaataagtg
     2701 cgtattttag ggaataactg cgtcgatttc cggataaatc gcggggattc cggctaattg
     2761 ttcaacagga aaggtagtca tctccgcccg attaacccca tcaccccctc tttataatta
     2821 accccgatgg gtatcgtcac cttccccaac tcgatcgagt tcccatagat cgcccggata
     2881 tgccccatcg ccaccaggta actccggtgc acccggataa acatccgcga cggcaacaac
     2941 ttctccaact cattcatcgt ctgatgcgtc acaagcgtat attccggcgt atggatcttc
     3001 aggtaatcct tcatcccctc cacatatagg atctccgaaa aattcacccg gaagaacttg
     3061 ctgttcgact tgacaaaaat atgatccccc tgcggcaacg ccggctcctc cggacccaaa
     3121 ccggaacccg gacccctccc tgcggccgcc ccgccgggta tcggatacaa cctcccatcc
     3181 aacaacttgt tcaccgcctt cgaaaatcgt tcaaaggaaa taggcttcag caggtaatcg
     3241 atcacattga actcataact ctccagcgca tactccgcat acgcggtggt cagcaccacc
     3301 agcggcggat cgaccagccc gcggagaaaa tgcagcccac tcatcccggg caaccggata
     3361 tccaggaaaa taacatccac ctcctgcgcc ttcagcgcct ccatcgcctc gggaccggtc
     3421 cggaatactc ccaccagttc catccggtcc atccgcgcca gatgactctt gatcacatcc
     3481 tgggccaggg tctcatcttc tacgatgatg cattttaaca agcggcaaat ttttttagga
     3541 ttaacgacac cgtaaaagta ctcttattta gaattacatc taaggtatga gaatccgggt
     3601 agaggttctc gagcctttcc cgggcattgc gcaatctcag cgcactggtc cgggtttcgt
     3661 ccttttccgt ctggatgatg tccaggtcat aggaggcggg gtagagcagc tccagcaggc
     3721 tccggctgtg ggcgataccc atcccgttga gcttcttggc ctgcaactcg aggtcggcat
     3781 ccgtactgtt gatgacgttc agcacaagct gctcgctccc cacccgaagg gtgatataaa
     3841 taaagggctc gccaaagctg tgatccatgg catgcttgaa accgttctcg atgaacgaga
     3901 taaaaagata gggctcgatc atcagcccgg cggtctgtcc ctccaccgtc agccggatat
     3961 cggccttatg ccgcagcttc tcgatctcga tatagttccg gatgaattcg atctccttat
     4021 ccagcggtat cttgtcttcg ttgcactcat agatgagata acgcatgata tccgccagcc
     4081 gcaccacgat ctccgccgtc cggtctgact tctgcatgct aagggaatag atgctgttca
     4141 gcgtattgaa gaggaaatgc ggatttagct gtgcccggag ataacgaagc cggaaaaaag
     4201 tattgtcctt ctgcatctcc accaactggt ccttctcgtc atccgccttc cggatataaa
     4261 agacagccat cgaaaaagcc atattcacgg ccgtccacca gccatcccgc agcaggtgcg
     4321 ccgggttcac cgaaaaatag agcttcatcg gtgcactggc catcttttcg aacagggaat
     4381 agacacaaag caaaaaagcc tggatgacaa gaaaagcgga gatggaaagc aggatgggga
     4441 accagcgccg tccccgtata gccagggcag accccatgag ccgcgaatag atgacatagg
     4501 ctggaaagac aagcaggttg cccgcaagca gaaaaggcca aagccaacga aaggggttca
     4561 atacccagca gctcaccggt tgcagcagca ccagcatgat ccaataaaac gtctccacct
     4621 gggggcgacg ctttcgaagc cggtcattat catattccat gggggtatag agatttgtct
     4681 caaaactacc ccggccatcc cctgtaataa aatcttttcg actaattcaa cccaacgttc
     4741 gatgaattag ccctattttc gcgcgacaca aacgcaaaaa ccgaaaacaa accggccctc
     4801 gggtcgtagc acaaacacat ggccaccaca agaataacca tcaacagcaa cggctcactg
     4861 aaactggaag gggatttcga actcgtagac cgcaacggca ccccctacgg actgggtggc
     4921 cgcgagatcg tcagcctttg ccgctgcggc cgctcccaaa acaaaccctt ctgcgacggc
     4981 tcccacaaag gccatttcga acacgacgcg actgctttcg atcttcctcc aaaaaaataa
     5041 taagattttt atatctttat ggagaacctc tgctctgatg attgcatacc aggacgaaca
     5101 gttattgctc ttgctcaaaa gcgacaatga agccgcgttt accgagattt accaccggta
     5161 ctggcagaga ttgttcatcg tggcaatgca tcgcctggcc aatacggagg aagcccgcga
     5221 actcgtccag gacatcttct gcaatctttg gaaacgaaga cactccctgc agatcgacta
     5281 ctcgctcaat acctatcttt ccgccgccgt caaatacgag gtgatcaacc ggctcgccca
     5341 aaagggccgg caacagcgat tccggagcaa actctcccgg gaatggctcg aagcctccaa
     5401 agacacagag aatcagctgc aattcaatga attgcaggac caactggccg ccctcgtcca
     5461 gaccctgccg gaaaaatgcc gcatcatttt ccaactcagc cgcgaaaaag gctattccca
     5521 aaaaaggatc gccgctgaat tgggtatcgc cgaaaaaacg gtggaagccc atctggccaa
     5581 cgctctcagg aaaatacgca ccggcctcag ccacctcttc ttctttcttt tttaattatt
     5641 ttttaacata gactaaggga tcggttccct tcgattggct atagtccaaa atagcccgat
     5701 atgaccgatc tgccttattc caatcggatc ctcgaactgg ctgataaatg gatcaaaggt
     5761 tccattacag aatcagaaaa aaatgaattc atcgactggt accggcgatt cgatgatcat
     5821 gaactcctgt tgacgcctga attcaaaccc ctcataaaag gactggaagc agacatgctg
     5881 gtcaatatcc ggcgacgcat cgcgggtgat gaatccccac ccatccggac gaccccatcc
     5941 gaacataagg gacggttggt taagataaag acctggcaga ttgccgccgc ggccatcctt
     6001 cttctcacgg cttccatgct ccccttcctg ctcgccagga aaacccgccg ggagaacgcc
     6061 gtcgccctgc aaaagcccgc tccggcgcca ccgccacgga ccgatatcct gccgggtagc
     6121 aacaaggcca tcctcaccct ggccaacggc tctacgatca ccctcgacag cgcccacaac
     6181 ggcaacctca cgcaacaggg gaataccctg gtggtaaagt ccgcgggcgg ttccttacga
     6241 tataatctgc ttagcaaaga ccagggtaaa gatcaaagca accaacaacg atccaatgcc
     6301 gttgcgtata atgtcctttc taccccccgg ggtggccaat atcaattggt cctgcccgac
     6361 ggaagcaggg tctggctcaa tgccgcgtcc tctatccgct atcccacggc cttcgcaggc
     6421 gcaaaccggg aagtggaagt cacaggcgaa gcctatttcg agatcgcaaa gaatgccgcc
     6481 atgcccttcc gcgtcctaac cgtcaaccac ctgggcgaca ccgacccgat ggccgtcgat
     6541 gtgctgggga cggatttcaa cgtcaatgct tatgcggatg aatccgtggc acggacaacc
     6601 cttgtcgaag gaatggtcag ggtttcaaaa gggaacagca agaccctcct gcaacccggc
     6661 cagcaatctg aactacagaa gaacggcgcc atcacccggg tagcggatgc cgatgtcgaa
     6721 aaagccgtcg cctggcgcaa tgggatattt gagttcggcg acgaagagct gccggtcatc
     6781 atgcggcaga tctcccgctg gtatgacgtc gatgtcgttt accagggacc ggtcccgacc
     6841 gaccgtttca caggccgggt aacacggaac accagccttt cgggggtgct gaagatcctg
     6901 aaactcagcg acgtacagtt cacggtcgcg gacaacaaga tcatcgtgca ttcataaaac
     6961 aaggagtaaa acaaaaaaac gatccgaaaa ggataaagat aaagctgaca aaaagcaaaa
     7021 accgggaacg ttggaggcgt ccccggcgac ggctgagtca gttgttgtaa accagtattg
     7081 cgactgttta ttcattaact caaaccaatc aaatttatga ttccgactgc ttattgccaa
     7141 atcggaccgg gaaccccgcc cttatcctct aacgatgctc cccggccact aaaaaaaatc
     7201 tggcttgtga tgaaaattac ggcttttcta ctgcttgcag cctgtattca tgtctcggcg
     7261 acgggcttct cgcaaaatat ctccctgtcg gtgcatgaag ccccgctgac aaaggttttc
     7321 cgggagatcc agcgccaatc gggctactcc ttctggtaca agacgcgtca actggacaag
     7381 gcggtaaaag ttaccctcga tctccggaac gccaccctcc aggaagccct caccaaggcc
     7441 ttcgagaacc agccgctcga cttcgttatc gttgaaaaga cggtagtaat tcgtccccgc
     7501 gacctggcag cggcccccgc tccacccccc attatcatca aaggacgggt ctccacggcc
     7561 tccggtaaag gcctcggcgg cgtcagcgtc accgttaaag ccaccaaaaa gggtaccgtc
     7621 accgacgaaa atggcgaata caccatcagc gtcgataaag gtgaagtcct cgtcttctct
     7681 tacgtaggct acgacacccg cgaagtaagg gtaggggacc agacttccat cgccgtcagc
     7741 ctcgtcgagg ccaacgcctc gctaaacgag atcgccgtca tcggttacgg ccgcgccagc
     7801 cggaaaagcc tggccagtgc gatcaatacc gtgaagagcg aagatctgaa taagggcgcc
     7861 atcaccgacg tgggccaact cttgcagggt aaggttcccg gcctgaacat cacggccagt
     7921 ggcgacccga atgcccccgc cgccgtcatc ctccgcggaa cgtctacact caacagcaac
     7981 cagacgcctt tctatgtcat cgacggagtg ccgggcgccg atatctccct catcgccccc
     8041 gatgacatcc aatccatcga cgtgctgaag gatgctgccg ccacggccat ctatgggaac
     8101 cgtgccgccg ccggcgtcat catcgtcacc acccgcaagg gtaagaccgg ccagatgcag
     8161 gtcgggtaca gcggttatgt aggcgccgaa gaggtgtcca gccggctgaa agtgatgaac
     8221 gcctcccagc tgcgcgcctt cctgtcaaag aataacgaag gcttttcccc caccgacgac
     8281 aagggcgcca acaccgattg gcaaaaggcc atcgaacgga cgggcgtatc ccacaaccat
     8341 aacctgtcct tcagcggcgg caacgagcat ggcagctaca tcgccagcat caactacgcc
     8401 gacaaggaag gtgtcctgaa aaacagcgac ctccggcgcc tcatcgcccg cctgtcggtg
     8461 gaacaatatg ccctcaacga caaggtgaa
//
LOCUS       DATGFG010001075         7742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1327417, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001075 DATGFG010000000
VERSION     DATGFG010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327417"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VKQ52_20420"
     CDS             complement(<1..592)
                     /locus_tag="VKQ52_20420"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ89636.1"
                     /translation="MSSPTLVSKLPQVGTTIFTVMSSLAAELGAVNLGQGFPDFPMSE
                     ELVSLVDQAMREGHNQYAHMNGLASLREVLAAKIAGLYGAAVSPDTQITVTPGGTYAI
                     YTALTTVLQPCGEVIVFEPAYDSYIPNIVINGAIPVRIDLRFPDYRIDWETVRQKITP
                     RTRMILLNTPHNPTGSILRPEDLRQLREIVAGTRILI"
     gene            complement(599..1318)
                     /locus_tag="VKQ52_20425"
     CDS             complement(599..1318)
                     /locus_tag="VKQ52_20425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLZ89637.1"
                     /translation="MSPYPLHTILFLDIETVSQYPDFTGVPEDWQELWRHKAENLIRD
                     KAPVTPEELYGRAAIYAEFGKVICISCGVLSGPPADRKLSLKSFYGDDEKELLQQFYV
                     LLHKWGSEGQKYLCAHNGKDFDFPYLCRRMIIHGMKIPSVLNLSGKKPWEIPHIDTME
                     LWKFGEYKNFISLNLLAHSLGVPTPKDDIDGSQVGTVYWVERDLPRIVTYCQKDVVTV
                     AQVYLRIHGETLIRPENICIK"
     gene            complement(1350..2432)
                     /locus_tag="VKQ52_20430"
     CDS             complement(1350..2432)
                     /locus_tag="VKQ52_20430"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HLZ89638.1"
                     /translation="MNKHHYVVIMAGGIGSRFWPMSRQDYPKQFLDILNTGRTLIQGT
                     FDRFSTFIPLENIFVVTSQEYAGIVKEQLPHLPVENILGEPSRKNTAPCIAYISFKLQ
                     QSDPKASLIVAPSDHLILDRTAFTKVCLEALSFVNKHNAFITLGIKPTYPNTGYGYIQ
                     YEQQSVSDNVYKVKTFTEKPNLELAKTFLASGDFLWNAGIFVWQVKNIIQAFEKYLPE
                     MYDVFVAEKDKFNTPEEREALQAIYPLCTNISIDFGIMEKADNVYLIPSSFGWSDLGT
                     WNSAYENLGKDYLENAVAGDNVIVIDATRCMVHAPNEKLVLLQGLDNVIVVDTPDVLL
                     VCKREKEQEIKEYVAEVKRNKGDQYL"
     gene            complement(2441..3418)
                     /locus_tag="VKQ52_20435"
     CDS             complement(2441..3418)
                     /locus_tag="VKQ52_20435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HLZ89639.1"
                     /translation="MSRATNILGIGRHIVRMEAAAVAALEQQLNGQFEKAVELIFSCK
                     GRVVISGIGKSAIIAQKIVATLNSTGTPAIFLHAADAIHGDLGMVQEQDVVILLSKSG
                     ESPEIKMLLSFIRGFGNPLIAMVGNLQSLLARQADIVLNTTIEQEAGTNGLQSLAPTS
                     STTVQLVMGDALAITLMEMRGFGSDDFARFHPGGTLGKRLYLRVQDVYVHNEQPRVTP
                     DTALREVIIEITSRRLGATAVTDEAGLLLGIITDGDLRRMLEKEGMRTGLRARDIMTT
                     SPKTVEPEALAIEALDLMRVHDITRLVVTSGNRYLGFIDLNNLIREGII"
     gene            3800..6079
                     /gene="recQ"
                     /locus_tag="VKQ52_20440"
     CDS             3800..6079
                     /gene="recQ"
                     /locus_tag="VKQ52_20440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HLZ89640.1"
                     /translation="MATTETKKTTARKTRTVSSKTTVKEKAKPSSPLLAQLQEHFGFD
                     SFKDNQDTIIQSLLSGKDTFVIMPTGGGKSLCYQLPAIISEGVAIIVSPLIALMKNQV
                     DLVRSYSSKDSVAHFLNSTLSKKQIKEVHEDLLNGHTKMLYVAPETLTKQENMEFFSD
                     LKISFFAVDEAHCISEWGHDFRPEYRRLREMMDQINPGIPVIALTATATPKVKSDIVK
                     NLGLRDPNIFISSFNRPNLYYEIQPKIKKDQTIKSIVRYIVQQKGKSGIIYTLNRKTT
                     EELADMLVANGIKAVAYHAGLDSKLRAERQDLFLNEDVQVIVATIAFGMGIDKPDIRF
                     VIHFNIPKSIENYYQETGRAGRDGLEGNCILYYSHKDVAKLEHLMKDKPLSEREVGAQ
                     LINEMVGYAESGVCRRKVLMSYFGEGYETENCGSCDNCLHPKEHVEARDHAVKVLKTI
                     KALDERFATGYVVNILTGKLTPQIQMFRHEGIAEFGIGKEDPEHLWNSLIRQLLLEDL
                     LKKDIEEYGVLKLTKKGEDYLRKPRSFRIVLNNLFEEANADDEEENTEASGSTSTDEK
                     LFELLKELRQKEAKKKALPPFVIFLETSLQDMATLYPTTTQELEKCQGVSKGKALRYG
                     KPFIELIARYVEENDIQKPDDFVMKSVVNKSVNKVYIIQQVDKKIPLETIAKNKDLRL
                     DALLEDMETIAASGTKLNLDYAVDEMLDEYEQDEIIDYFKSCETSSLQVAQQELSEGN
                     FTWEQLKIMRIKFLSEYGM"
     gene            6319..6391
                     /locus_tag="VKQ52_20445"
     tRNA            6319..6391
                     /locus_tag="VKQ52_20445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6352..6354,aa:Phe,seq:gaa)
     gene            6470..6838
                     /locus_tag="VKQ52_20450"
     CDS             6470..6838
                     /locus_tag="VKQ52_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89641.1"
                     /translation="MRNAFLLLACMGSVVLAHAQANSPVTPENRITITVSNNSLSVSC
                     KSKLVTIHSNPELDNCLQTIIPGLDHPSILLDVPSDMDREKLRTIGVTLEKFHCPVMS
                     FRREDSIKPAASRWLDSTGH"
     gene            complement(6955..7344)
                     /locus_tag="VKQ52_20455"
     CDS             complement(6955..7344)
                     /locus_tag="VKQ52_20455"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ89642.1"
                     /translation="MANLTYVSPCFIVSSLKDSVSFYVDKLGFEIRYIGPPDDPFFAI
                     VGRETISIMLKAIAPDIHPVPNHTRHEWARLDAFIYAADPDAIFEEYRSRGLAFHQSI
                     KDDGDGLRGFEIADADGYILFFGRPIS"
     gene            7393..>7742
                     /locus_tag="VKQ52_20460"
     CDS             7393..>7742
                     /locus_tag="VKQ52_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89643.1"
                     /translation="MQYLKQPLSTLLLATSLLLAALSSTAQQPTTQQPATQKLYVPNF
                     DSSWVREQEPFRIAGNLYYVGSYDLTSYLITTPKGHILINTGIPGSDSMIRKHVEALG
                     FKFSDIKILLATHA"
BASE COUNT         1884 a         2150 c         2008 g         1700 t
ORIGIN      
        1 gaataaggat cctggttccc gcaacgatct cccgcaactg acgcaggtct tccggtctta
       61 aaatagaccc cgtggggtta tgcggtgtat tgagcaggat catccttgtc cgtggcgtta
      121 tcttttgtct caccgtctcc cagtcgatcc ggtaatccgg gaagcgcagg tcgatccgca
      181 ccgggatcgc accgttgatg acgatattgg gaatgtaact gtcgtaggcc ggctcgaaga
      241 cgatcacctc gccgcaaggc tggagaacgg tggtaagcgc ggtatagatc gcataggttc
      301 ccccgggagt gaccgtgatc tgggtatcgg gacttacggc cgcaccatag agaccggcga
      361 tcttcgcggc gagcacctcc cgcagggacg ccagcccgtt catatgcgca tattgattat
      421 gcccctcgcg catggcctgg tcgacaagcg acaccagctc ctcgctcatc gggaagtccg
      481 ggaagccctg gccgaggttc accgcaccga gttccgcggc aagcgaagac atgaccgtga
      541 aaatagtcgt tcctacctgg ggaagtttgc tgacaagggt gggagaactc atggaggctt
      601 atttgataca aatgttttcc ggtcggataa gggtctcgcc atggatccgc aggtagacct
      661 gcgctaccgt gaccacgtcc ttctggcaat aggtcacgat ccgtggcaga tcacgctcga
      721 cccagtagac cgtgcccacc tggctgccgt cgatatcatc cttgggggtt ggtacgccaa
      781 gcgaatgggc aagcaggttg agggaaataa agttcttgta ttctccgaat ttccagagct
      841 ccatcgtatc gatatgcggg atctcccagg gttttttccc ggaaagattg agcaccgagg
      901 ggatcttcat tccatggatg atcatccggc ggcagagata ggggaaatcg aagtccttgc
      961 cattatgggc gcagaggtat ttctggcctt ccgatcccca tttgtgcagc agcacgtaga
     1021 attgctgcaa aagctctttt tcgtcgtcac cgtaaaagga tttgagggaa agcttccggt
     1081 cggcgggcgg tccggacaac acgccgcaac tgatacagat cacctttcca aactccgcgt
     1141 agatagcggc ccggccatat agttcttcgg gggtgacggg cgccttgtcc cggatgaggt
     1201 tctccgcctt gtgacgccac aattcctgcc agtcttccgg tacgccggta aagtcggggt
     1261 attgcgacac ggtttcgatg tccaggaaaa gaatggtatg cagtggatag ggagacatgt
     1321 cggttgagac gaacggttct gtccgtccct taaagatact gatctccttt attcctcttt
     1381 acttctgcga catattcttt gatctcctgc tctttctcgc gtttgcagac aagcagcaca
     1441 tcgggggtat cgaccacgat gacattgtcc aggccctgga ggaggaccag tttttcgttg
     1501 ggggcgtgca ccatacaccg ggtggcatcg atcacgatga cgttgtcgcc ggcgacggca
     1561 ttctccaggt agtccttgcc gaggttctcg taggcgctgt tccaggtgcc caggtcgctc
     1621 cagccaaagg aggaggggat gagatagaca ttatccgcct tctccataat gccgaagtcg
     1681 atggagatat tggtgcaaag ggggtagatc gcctgaagcg cctccctttc ctcgggggtg
     1741 ttgaacttgt ccttttccgc gacaaagaca tcgtacatct cgggcagata tttttcaaag
     1801 gcctggatga tattcttcac ctgccagaca aagatccccg cgttccacag gaaatcgccg
     1861 ctggccagaa aagtcttggc cagttcgaga ttgggtttct cggtaaaggt cttcaccttg
     1921 tacacattat ccgaaacgga ttgctgttca tactggatat acccatagcc ggtattgggg
     1981 taggtgggct ttatccccag cgtgataaaa gcattatgct tgttgacaaa gctgagcgct
     2041 tccaggcaga ctttcgtgaa ggccgttctg tcgaggatca ggtgatctga cggggctacg
     2101 ataagggaag ccttgggatc cgactgctgc agcttgaaag agatataggc gatacagggg
     2161 gcggtattct ttctcgaggg ttcgccgagg atattttcca cgggcagatg gggcagctgc
     2221 tcttttacga tcccggcata ctcctgggaa gtgacgacaa agatattctc aagcgggata
     2281 aaggtggaaa agcgatcgaa ggtcccctgg ataagtgtcc gtccggtgtt caggatatcg
     2341 aggaactgct ttggatagtc ctggcggctc atcggccaga aacggctgcc gatgcccccg
     2401 gccatgataa cgacataatg atgtttgttc ataaagtcct ttaaattatt ccctctcgta
     2461 taagattgtt aaggtctata aagcccaggt atcggttacc gctggtgacg accagccggg
     2521 tgatgtcgtg aacacgcatg agatcgagcg cttcgatggc cagggcctcc ggctccactg
     2581 tcttggggct ggtagtcatg atatccctgg ccctgagccc ggtacgcatg ccttcctttt
     2641 ccagcatccg gcgaaggtcg ccatcggtta tgatgcctaa cagcagtcct gcttcgtcgg
     2701 tgacggccgt agcgcccaac cgccgcgacg tgatctcgat gatcacctcc cgcagggcgg
     2761 tatccggggt cacccggggc tgttcattgt ggacatacac atcctgtacc cgcaggtaga
     2821 gccttttgcc aagggtaccc ccgggatgga agcgggcgaa gtcatcgctc ccaaatccgc
     2881 gcatttccat aagggtgatg gccagggcat cgcccatcac cagttgtacc gtcgtactgc
     2941 tggtgggcgc gagactctgc agaccattcg tcccggcctc ctgctcgata gtggtgttga
     3001 ggacgatatc cgcctggcgg gctaacaggg attgcaggtt gccgaccatc gcgatcagcg
     3061 ggttaccaaa cccgcggata aaggagagca gcatttttat ttcggggctt tccccgctct
     3121 tgctcagcag gatgaccaca tcctgctcct ggaccatccc caggtcgcca tggatggcgt
     3181 cggcggcatg gaggaatatg gcaggggtac cggtggaatt gagggtggcg acgatcttct
     3241 gcgcgatgat cgcactcttg ccgatcccgc tgatgacgac ccggccctta caggaaaaga
     3301 tcagctccac tgccttttcg aattgcccat tcagctgctg ctccagggcc gcgaccgcag
     3361 ccgcctccat ccggacgata tgccttccta tcccaagaat attcgtagcc ctgctcataa
     3421 ataagttgca aacctaaaat aagtcgcaaa cctacggtaa aatgcgtaaa tcgcctcctt
     3481 cacgccgccc cttagcaatt ttatagaatt ttggggtagt cgaattcgca atcggctgaa
     3541 aaacaaaaat atacctattt tatgcgtcaa tcttacatag taacattctg ccaatccctg
     3601 tcttttgcgc gccaatttta acacaatccg ctagctcctt accccctttt tttgtaactt
     3661 cgccatccct taaattagtt caccctatta atccgaactc atgggtaaac tatattccgg
     3721 ctaccttaca ttattcttgc caggagaaca tcaagggctc tcaagatcag ataacaagtg
     3781 aggaagaaaa tttttgagca tggcaacaac cgaaacaaaa aaaaccaccg ccagaaaaac
     3841 caggaccgtt agtagcaaaa cgactgtcaa agaaaaggcc aaaccatcct cccccctgct
     3901 ggcccaactg caggaacatt ttggcttcga ctcctttaaa gataaccagg acacgatcat
     3961 ccaaagctta ttatccggga aggatacatt tgtgattatg cccaccgggg gcggtaagag
     4021 tctctgctac cagctgcccg ccatcatcag cgaaggcgtg gccatcatcg tcagccccct
     4081 catcgcgctg atgaagaacc aggtagacct cgtccgcagc tatagcagca aggacagcgt
     4141 ggcccacttt ctcaattcta cgctttcgaa gaaacagata aaggaagtac atgaagacct
     4201 gctcaacggg cataccaaaa tgctgtatgt agccccggag acgcttacca agcaggagaa
     4261 catggaattc ttcagcgatc tgaagatctc cttctttgcc gtggatgaag cccactgtat
     4321 ctcggagtgg ggtcatgatt tccgcccgga ataccgccgg ctgcgggaaa tgatggacca
     4381 gataaacccc ggcatcccgg tgatcgcgct gacggccacc gccacgccaa aggtaaaaag
     4441 cgatatcgta aagaacctcg gcctgcgcga ccccaatatt ttcatttctt cttttaaccg
     4501 gcccaatctc tactacgaga tccagcccaa gataaagaag gaccagacca taaagagtat
     4561 cgtccgctat attgtccagc aaaagggcaa gagcggcatc atctatacgc tcaaccgaaa
     4621 gaccaccgaa gagctcgccg acatgctcgt ggccaacggg ataaaggccg ttgcctatca
     4681 cgcgggtctc gacagcaagc tccgcgccga aagacaggac ctcttcctca acgaggacgt
     4741 ccaggtcatc gtcgccacca tcgccttcgg gatgggcatc gacaagccgg atatccggtt
     4801 cgtcatccac ttcaatattc ccaaatccat cgagaactac taccaggaga ccggacgcgc
     4861 cggtcgggat ggtctcgagg gtaactgtat cctgtattac tcccacaaag acgtcgccaa
     4921 gctggaacac ctgatgaagg acaagccgct cagcgaacgc gaagtgggcg cccagctcat
     4981 caacgagatg gtcggctatg ccgaaagcgg cgtctgccgc cggaaggtgt tgatgagtta
     5041 ttttggtgaa gggtacgaaa cggagaactg cggcagctgt gacaactgtc tgcatccgaa
     5101 ggagcatgta gaagcccgcg accatgccgt caaggtgctg aagacgataa aggccctcga
     5161 cgagcgcttt gcaacgggat atgtcgtcaa cattctcacc gggaaactga cgccccagat
     5221 ccagatgttc cgtcacgagg gcattgcgga attcggcatc ggcaaggagg accctgaaca
     5281 tctctggaac tcccttatcc ggcaattgct gctggaagac ctgctgaaga aggacatcga
     5341 ggaatacggc gtgctgaagc tgacgaagaa gggcgaggac tacctcagga agccccgctc
     5401 gttcaggatc gtgctgaaca atctcttcga agaggccaac gccgatgacg aagaggagaa
     5461 caccgaagcg tcgggctcca cgtccaccga cgagaagctg ttcgaactgt taaaggagct
     5521 ccggcaaaaa gaagccaaaa agaaggctct tccgcccttc gtcatcttcc tggagacctc
     5581 gttgcaggat atggctacgc tctatcccac cacgacccag gagctggaaa aatgccaggg
     5641 cgtcagcaag ggcaaggccc tgcgctatgg caagcccttt atcgaactga tcgcccgcta
     5701 tgtcgaagag aacgacatcc agaagcccga tgattttgtg atgaagagtg tcgtcaacaa
     5761 aagcgtgaac aaggtctata tcatccagca ggtggacaaa aagatcccgc tggagaccat
     5821 tgccaagaat aaggatctgc gtctcgacgc cctgctggag gacatggaaa ccatcgccgc
     5881 cagcggcacc aagctcaacc tcgactatgc cgtcgacgag atgctcgacg agtatgagca
     5941 ggacgagatc atcgattact tcaaaagctg cgaaaccagc agcctccagg tcgcccagca
     6001 agagctgtcc gaaggcaatt tcacctggga gcaattgaag atcatgcgga tcaagttcct
     6061 cagcgaatac gggatgtaag ccggccccgg catcgcggca tcgagcccct ggcgtcacct
     6121 ctcgggcgca ccccggccgt cgctcgcccc ggcacgccat cggacctgac cctcaaaatt
     6181 ttatggaatt tccgcggctt ttatttcgaa atttccgcgg gaaaacctat ttttgccacc
     6241 cgatcgaaaa ttcggccttc ccggatccaa ctcccggttc gcgcccggcg aaggctcacc
     6301 gatgatcgat caaaggatgg tgcggtagct cagtcggtag agcaaaggac tgaaaatcct
     6361 tgtgtcggcg gttcgatccc gccccacacc acagctttaa aggtcgcagc aatgcggcct
     6421 ttttttatgt aatctccggc cggccggcat cctcctgcta aactccaata tgagaaatgc
     6481 tttcctgctc ctcgcatgca tgggatctgt cgttcttgcc cacgcgcaag ccaatagccc
     6541 tgttaccccg gagaaccgta taaccattac tgtatcaaac aacagcctgt ctgtctcgtg
     6601 caaaagcaag ttggtcacca ttcacagtaa cccggaattg gacaactgcc tgcaaacgat
     6661 catcccgggc ctggatcacc catctatcct gctggatgtt ccgagcgata tggatcggga
     6721 aaaacttcgc accatcggcg tcaccctgga aaagttccat tgtcccgtca tgagctttcg
     6781 gcgggaagac tctattaagc ctgcggcaag ccgatggttg gattcaaccg gccattagaa
     6841 gcgccctctc atactggagg ttccggggtc cgcacaaaaa tgaagcgatt atgagacaat
     6901 tggcttcaaa atataggatc atgagccgat tagccgcctt aatcggctca tatcttacga
     6961 aatcggccgc ccgaaaaaca ggatataccc atcagcgtct gctatttcaa atccgcgtaa
     7021 cccatcgcca tcatccttga tcgattgatg aaacgccagg ccgcgggaac gatattcttc
     7081 aaagatggca tcgggatccg ccgcataaat aaatgcatcc aggcgcgccc attcatgtct
     7141 tgtatgattg ggcaccgggt gaatatccgg ggcgattgcc ttaagcataa tagaaatcgt
     7201 ctcgcgccct acgatcgcaa aaaaaggatc atcgggcggc ccgatatacc ggatctcgaa
     7261 tccaagctta tcgacataga aggataccga atccttcagg cttgaaacta taaagcaggg
     7321 ggagacatag gtcaggttgg ccataaatgg tatttcccga atgtacgttt tttattatct
     7381 tccggctctc ttatgcaata cctaaaacaa ccactgtcga ccttgctatt ggcaaccagt
     7441 cttctcctcg ccgccctgag ttccaccgct caacagccaa cgacccagca accggcaacc
     7501 caaaaactat acgttcccaa cttcgactcc agctgggtca gggagcagga gccctttcgg
     7561 attgccggca acctatatta tgttggctcc tacgatctga caagttacct catcacgacg
     7621 ccaaaggggc atattctcat caacacgggg ataccgggat cggactctat gatccgcaaa
     7681 cacgtcgaag cattgggatt caaattctcc gatatcaaaa tactcctcgc aacgcatgct
     7741 ca
//
LOCUS       DATGFG010001076         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_62617, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001076 DATGFG010000000
VERSION     DATGFG010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62617"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1910)
                     /locus_tag="VKQ52_20465"
     CDS             complement(135..1910)
                     /locus_tag="VKQ52_20465"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ89644.1"
                     /translation="MRTLRLIWSISKKWTTLTLVAIVVESGLYLLLLYIFKHLINEVT
                     RATPFDTVKVHRVEWYLIIITAGTIIYVIVKALSTLTTEIQAQRVKESIDEKIHECAV
                     NLDLAFYESPAYFDTMKRARDAGPEAPNNVVLNLADIAKNGLTLVAIGSILISISWLL
                     LPLLALFVAPTLLVRIRYAEQMYQWRRRQTPVERKAAYLSSLITEDTSAKEIRGYGLG
                     SHIRQLYTGIRMGLMRERLAMTRRNTFKELLTNIMATLGLFACIAYLCFSMIRGSISV
                     GDMSLFLIVFPQSYGILQLMSQGISGLYQNNIFINNLFELFDLRATLKEPEHPLPIPE
                     SEAFDLEVENMSFTYPHAKEPALRNVSLRVPPGKIIAIVGLNGAGKTTLIKLLCRLYD
                     PTDGCIRMDGVDIRAFRSAEYRKHISAVFQDFGRFNITAAENIRWGDIDGQRPENEIE
                     EAARKSGAHEYIRRFPEGYGTMMGRIFENGREVSIGQWQKLAIARSFYSNSRFVIFDE
                     ATSALDAVSEEELFQSFRQRIGNRAALIISHRLSAVRHADHIYVMGEGRVQQHGTHEE
                     LVAMGGTYSKLFLQKPVESDGAEKS"
BASE COUNT          462 a          610 c          504 g          443 t
ORIGIN      
        1 cccggttgat ccggtcctta tacacaacga ccccgagccc ggcctcctgc aacaggggaa
       61 tgctccgggc ccatagctct tcgctgccgc cccattcctc gatacaggta gatatgatcg
      121 caattgttgg catattagct cttttccgcg ccatccgact ccaccggttt ttgcaggaat
      181 aatttggaat aagttccccc catcgctaca agctcctcat gggtcccatg ctgctgtacc
      241 cgtccttcac ccatcacata gatatgatcc gcatgccgca ccgcggacaa gcggtgactg
      301 atgatcaacg ccgcccggtt gccgatccgc tgccggaacg actggaacag ctcctcctcc
      361 gacaccgcat cgagtgcgct cgtcgcctcg tcgaagatga caaaccggct attgctgtaa
      421 aaagaccggg ctatcgccag tttctgccac tgaccgatac tcacttcgcg cccgttctcg
      481 aagatgcgcc ccatcatcgt cccataaccc tcggggaatc gccggatata ctcatgcgcc
      541 cccgacttcc gcgccgcctc ttcgatctca ttctccggcc gttgaccatc gatatcgccc
      601 cagcggatat tctcggccgc ggtgatattg aaccgcccga aatcctggaa aacggcgctg
      661 atatgcttcc ggtactccgc actgcgaaag gcgcggatgt ccaccccatc cattcggatg
      721 cacccatcgg tcgggtcata aagccggcat aagagcttga tcaacgtcgt tttgccggcg
      781 ccgttcaacc cgacgatcgc gatgatcttc cccgggggca cccgcaagga tacattccgc
      841 agggccggct cctttgcatg cgggtaggta aaactcatat tctccacttc caggtcaaaa
      901 gcctcggatt cgggtatggg taagggatgt tcgggttcct ttagcgtcgc ccgcagatcg
      961 aacagctcga agaggttatt gataaagatg ttgttctggt agagcccgga gatgccttgc
     1021 gacatcagct ggaggatgcc gtaggactga gggaaaacga tgaggaacag ggacatatcc
     1081 cccacgctga tactacccct gatcatgctg aaacagaggt acgcgataca ggcaaaaagt
     1141 cccagggtcg ccatgatatt cgtcagcagc tccttgaagg tattgcgacg ggtcatcgcc
     1201 agccgctccc gcatcaaccc catccggata ccggtataca actgacggat atggctaccc
     1261 agcccatatc cccggatctc cttggccgag gtatcctcgg tgatcaggct gctgagatag
     1321 gccgccttcc gctccacggg tgtctgccgc cgccgccact gatacatctg ctctgcatac
     1381 ctgatccgca ccagcagggt aggcgccaca aaaagcgcca gcaggggcaa caataaccag
     1441 cttattgaaa taagaataga cccgatggcc accagggtca gaccgttctt ggcgatatcg
     1501 gcaaggttca ggacgacatt gttcggcgcc tcggggcctg cgtccctggc acgtttcatc
     1561 gtgtcgaaat aggccgggct ctcatagaag gcaagatcga ggttcaccgc gcattcatgt
     1621 atcttttcat cgatgctttc ctttacccgc tgtgcctgga tctcggttgt aagggtcgat
     1681 aacgccttca caatgacata gataatggta cccgccgtga tgatgataag ataccattcc
     1741 acccgatgca ccttcaccgt gtcaaacggc gtcgcccggg tcacttcatt gatgagatgc
     1801 ttgaaaatat ataataacaa caggtacaac ccactttcaa caacgatcgc caccagggtc
     1861 agcgtagtcc atttcttcga gatcgaccag atgagacgca gtgtcctcat caaatgaagg
     1921 ttggttttaa tctgctggac ccgatgacgg attgacccga tcatgaatgc cggaatgttt
     1981 atagggttat ataatagaag cggagaaccc gcccaaaat
//
LOCUS       DATGFG010001077         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1331066, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001077 DATGFG010000000
VERSION     DATGFG010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331066"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VKQ52_20470"
     CDS             <1..773
                     /locus_tag="VKQ52_20470"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89645.1"
                     /translation="MHTGRIGHYDNLPEGAQLIGASGIKAGGRIFTDKSGMQEHSTPT
                     ALTSDGVAAVIEGFVQAARNGVAAGFDGIELHGASGYLIEQFLHPHVNTRTDEYGGSI
                     EGRARLAIRLAKDIADAIGREKVGIRFSPYSQLGDLQPYADQEVHETYTYLAAQLEKI
                     GIAWLHIAANVPVPQKTFDGIRSAFSGTIILCGRLTPATAEAALHAGFADLVAFGRSF
                     LSNPDLDKRIAANAPLNEPDLTTFYTPGAKGYTDYPTL"
     gene            complement(781..2241)
                     /locus_tag="VKQ52_20475"
     CDS             complement(781..2241)
                     /locus_tag="VKQ52_20475"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLZ89646.1"
                     /translation="MNKVCFWIVLLLLARVGVAQSYSPEVEKKIKEVETHLGTGIQIT
                     GHPWYTLQERMAYYHVHGVSIAVVHNHRIEWARGYGWADTAEKRPVTVHTLFQAASIS
                     KSLNAVGLLRLTQEKKIDLYADINTYLRSWKFPYDSLSHGKTISVANLLSHTGGLTVH
                     GFPGYERGVAIPTVVQVLDGAPPANTKAVRSMYAPGIKSEYSGGGTTISQLIVMDVTQ
                     EPYDRWMADHVLRPMGMTESFYTQPAPADKAGVLATAYHEDGSAVPGKYHIYPEEAPA
                     GLWTNPTDLCSYIIETQLSYAGRSAKVLDQSFTRLRLTPYVDSTAALGVFISKAGDVR
                     YFQHGGANEGFRSQYYGSEDGGEGVVVMVNSDNGAVMSEIIAGVATVYGWTGFYHPVV
                     KTIYPMKPEQLAAYEGRYRYRGDRPLELQVVAKGDHLVVTALWEKHDDDFYPESETSW
                     FRMNGNFVIQMEKGADGKVKTISGGGKDFFDRVGEK"
     gene            complement(2287..>2583)
                     /locus_tag="VKQ52_20480"
     CDS             complement(2287..>2583)
                     /locus_tag="VKQ52_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89647.1"
                     /translation="AFYVLAYVRLGAISRHYDPLYPADYLDHRETHRSAGLNVGATMT
                     GDKHWGLDINAGIFVKKKSIAETYLHGPEMAKESVEPGFRLSVNICYWFKRAKV"
BASE COUNT          549 a          895 c          620 g          519 t
ORIGIN      
        1 tgatgcacac ggggcgcatc gggcactatg ataatttgcc ggaaggcgct caattgatcg
       61 gcgcatcggg gataaaagca ggcggccgga tcttcaccga caaatccggt atgcaggagc
      121 attcgacccc caccgccctt acctccgacg gcgtcgccgc ggtcatcgaa ggctttgtcc
      181 aggccgccag gaacggcgtc gcggcaggct tcgacggcat cgagctgcac ggggccagcg
      241 gctaccttat cgagcaattc ctgcatcccc atgtcaatac aaggacggat gaatacggtg
      301 gaagcatcga aggaagggcc aggctggcca tccgtctggc aaaggacatt gccgacgcca
      361 tcggcaggga gaaagtaggt atccgctttt cgccctatag ccaactcggc gatcttcagc
      421 cctacgcgga tcaggaggtc cacgaaacct acacctatct tgccgcccaa ctggaaaaga
      481 tcggcattgc ctggctccat attgccgcta acgtgccggt cccccaaaag accttcgacg
      541 gcatccggtc cgctttttcc ggcaccatca ttttgtgcgg ccggctgacg cccgccaccg
      601 cggaagcggc cctgcacgcc ggttttgccg acctggtcgc ctttggccgg tcctttctct
      661 ccaaccccga cctcgacaaa cggatagccg caaacgcccc tctgaacgag cccgatctta
      721 ccacttttta tactcccggg gcaaaaggct acaccgatta cccgaccctg taacccgccc
      781 tcacttctcc ccaaccctgt caaaaaaatc ctttccccca cccgatatcg tcttcacctt
      841 cccgtcggcg cccttctcca tctggatcac aaaattccca ttcatcctga accagctcgt
      901 ctccgactcg ggatagaaat catcatcgtg cttttcccac aaggccgtca ccaccaggtg
      961 atcccccttc gccaccacct gtagttccag cggccggtct ccgcgatagc ggtatcgccc
     1021 ctcataggcc gccaactgct ccggcttcat cgggtagatc gtcttcacca ccggatgata
     1081 aaaccccgtc cacccataca ccgttgccac cccagcaatg atctcgctca tgacggcccc
     1141 gttatcactg ttcaccatca ccacgacacc ctcgccgcca tcctccgatc cgtaatattg
     1201 tgaacgaaac ccttcattcg cgcccccatg ctgaaaatac cggacatcgc ccgccttact
     1261 gataaacact cccagtgcag cagtgctatc cacatacggc gtcagccgca gccgcgtgaa
     1321 cgattggtcc agcacctttg ccgaccggcc ggcataggaa agctgcgtct cgataatata
     1381 gctgcacagg tctgtcggat tcgtccagag gcccgccggc gcctcctcgg gatagatatg
     1441 gtacttcccc ggtacggccg acccatcctc atgatacgcg gttgccagca cacccgcctt
     1501 gtccgccggc gccggctggg tatagaagct ctccgtcatc cccatcggcc gcaacacatg
     1561 gtcggccatc cagcgatcat agggctcctg cgtcacatcc atcacgataa gctgcgagat
     1621 cgtggtaccc ccaccggaat actccgactt gatgcccggg gcatacatac tccggacagc
     1681 cttcgtattc gccggcggcg ccccatccaa cacctgcacc accgtcggta tcgccacacc
     1741 ccgctcgtac cccgggaaac catgcaccgt aaggcctcct gtatgactca ggagattggc
     1801 gacactgatc gtcttgccat gcgaaaggga atcataggga aacttccatg agcgcagata
     1861 ggtgttgatg tcggcataca gatcgatctt cttctcctgc gtcagccgaa gcaacccgac
     1921 cgcgttcagc gatttactga tcgacgcggc ctggaacagc gtatgcaccg tcaccggccg
     1981 cttctccgcc gtatccgccc agccataccc ccgtgcccac tcgatccggt ggttatgcac
     2041 aacggcgata ctcaccccat ggacatgata atacgccatc cgctcctgta acgtgtacca
     2101 gggatggccc gtgatctgga tccccgtacc cagatgggtc tccacttctt ttatcttctt
     2161 ctccacctcg ggcgaatacg actgggcaac ccccacccgc gccaggagta aaaggacgat
     2221 ccaaaagcaa accttattca tagaaaatga tttatccagg ttaagacgcg cccccgccgc
     2281 gccgggttac accttcgccc gcttgaacca ataacaaata tttacggata gccggaaccc
     2341 tggttccacg gactctttcg ccatttcggg gccgtgcagg taagtctccg cgatgctctt
     2401 cttttttaca aaaatgccgg cgttgatgtc gagcccccag tgcttatcac ccgtcatggt
     2461 cgctcctaca ttgagccccg ccgatcgatg cgtctcccgg tgatcgagat agtccgcggg
     2521 gtacagggga tcatagtgcc tcgagatcgc gcccagccgg acataggcca ggacgtaaaa
     2581 agc
//
LOCUS       DATGFG010001078         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1438672, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001078 DATGFG010000000
VERSION     DATGFG010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438672"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VKQ52_20485"
     CDS             <1..1137
                     /locus_tag="VKQ52_20485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HLZ89648.1"
                     /translation="AFFAQTFAHHFLLPRPYFWPLKHRHMLHAVLDAYGFPADCSVTP
                     HGSGLINRTWIVKNGKDRFILQRINQDVFPAPALIAENIEMVGDYLSTHYPDAVFPRP
                     VPATTGDTLVSTGPDGFFRLYPFIEDSITIDVVDQPQQAFEAARQFGAFSRMMAGFPA
                     ERLHETLPHFHDLSLRYRSFREALQKGRPERLRKAGELIGYLEAQNHLVTKYQQILTT
                     PAFRLRVTHHDTKISNVLFNRSGKGICVIDLDTVMPGYFISDVGDMFRTYLSPVTEEE
                     KDITLIRIRLEYFEAIVQGYLEEMCEVLTPDEKKAFVYAGEFMLYMQALRFITDYLLG
                     DTYYGSKYEGHNYVRALNQATLLQRFSEQSTSLKNIASRVLTAL"
     gene            1354..>2883
                     /locus_tag="VKQ52_20490"
     CDS             1354..>2883
                     /locus_tag="VKQ52_20490"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ89649.1"
                     /translation="MTKKAALLCSFLLVCCCLVFGQTQTITGKITDESTKQPVSGATI
                     SVKGAKRSTTADANGAFSLPVPAGKVTLVISSIGFATKEILADAGSSLDIQMSQDTRQ
                     LGEVVVTALGITRQSKTLVYAAQTVAPGQLTGVRSTDNFLGSLSGKAANVQFTQGSGG
                     LGSGAVIVLRGNRSIAPAYGGNNANPNNALIVVDGVPINNTTFLTGNNDFGSIQSSDG
                     ASDINPDDIESMTVLRGASAAALYGSQAGNGVIVISTKKGHKNRTDVTINSGIGFERP
                     FALPQFQDSYGQGNTGLIATDSSQSGASWGAKMTGQSYIDYLYKRNTYSPQPDNVKKF
                     FNTGLSLNNSVGVSIGGDKTQSYFSYTNNYAKGITPSNDMNRHIFDYRITSDINKKFS
                     VDAKATYILQIINGRPRTGEENSPVFDVYQQPRSISYDEAKNNFQTFDAFGVAKPANW
                     AGTNSSIYQSPYWMTNNTSISENRERAIGFAALKYNITPWLNIRGSANIDRTSDEIQQ
                     KY"
BASE COUNT          721 a          997 c          625 g          540 t
ORIGIN      
        1 gccttttttg cgcaaacgtt tgcgcaccac ttcctcctcc cccggccgta cttttggccg
       61 ctcaaacacc gtcacatgct gcatgccgtt ttagacgctt atggattccc cgccgattgc
      121 tccgtcaccc cccacggcag cggtctcatc aaccggacat ggatcgtcaa aaacggtaaa
      181 gacaggttca tcctgcaacg tatcaaccag gacgtcttcc ccgcaccagc cctcatcgct
      241 gagaacatcg aaatggtagg cgattacctc tcaacccact accccgacgc cgtcttcccc
      301 cggccggtgc ccgcaaccac aggcgatacc ctggtgagca ccggccccga cggcttcttc
      361 cgcctctacc cgtttatcga agactccatc accatcgacg tcgtcgacca gccgcaacaa
      421 gccttcgaag cagcaagaca gttcggggcc ttcagccgaa tgatggccgg cttccccgcc
      481 gaacgactcc acgaaaccct gccgcatttt cacgacctct ccctccgcta tcgctccttc
      541 cgcgaagccc tgcaaaaagg ccgccccgag cgccttcgca aagcaggaga gctgatcggc
      601 tacctcgaag cacaaaatca tctcgtcaca aaataccagc agatcctcac aacccccgcc
      661 ttccggctcc gcgtcaccca ccacgacaca aagatcagca acgtcctctt caaccggtcg
      721 gggaaaggca tctgcgtcat cgatctcgat accgtaatgc ccggctattt tatcagcgac
      781 gtaggcgaca tgttccggac ctacctctct cccgtaaccg aggaagaaaa agatatcacc
      841 ctcatccgta tccgcctgga atacttcgaa gccatcgtcc agggctacct cgaagaaatg
      901 tgcgaagtcc tcacccccga tgagaaaaag gccttcgtct acgccgggga attcatgctc
      961 tatatgcagg ccctgcgctt cataaccgat tacctcctcg gcgacaccta ctatggcagc
     1021 aaatacgaag gacacaacta tgttcgcgcc ctcaaccagg ctaccctgct gcaacgcttc
     1081 agcgaacaat ccacctcatt gaaaaacatc gcctcccggg tcctcaccgc actctgaccg
     1141 cagtcataag ccccacacaa caacatccgt tcccgctcct ctcgtgagcg cattctgcta
     1201 aactgtagcc atattgcagt ttagcatcat attcaacgca gcaattccgc agttacccac
     1261 acactaagcg ctccgcaaac gtttgcataa tgcaccgttt ccttatagaa taatcctagc
     1321 tttacggcta taaccaatct ctaaattttg cttatgacaa aaaaagcagc gctattatgc
     1381 tccttcctgc tggtttgttg ttgcctcgtc ttcggccaga cacaaactat cacagggaag
     1441 atcacggacg agtctaccaa acaaccggta tcgggagcga ccatctccgt gaaaggtgca
     1501 aaaaggtcaa ccaccgccga cgcaaacggc gccttttccc tgcccgttcc ggcaggcaaa
     1561 gtgaccctcg tcatctcctc catcggattt gccaccaaag agatcctcgc ggacgcgggc
     1621 tcctccctgg acatccagat gtcccaggac acccggcaac tcggtgaagt agtcgtcacc
     1681 gccctcggca tcacccgcca gtccaaaacc ctggtatatg ccgcgcaaac ggtggcgccc
     1741 ggtcaactga caggggtcag atccacggat aacttcctcg gctccctctc ggggaaagca
     1801 gccaacgtcc agtttaccca gggctccggc ggcctcggca gcggcgccgt catcgtcctc
     1861 cgcggtaacc gctccatcgc accggcgtac ggaggaaaca acgctaaccc caacaacgcc
     1921 ctgatcgtcg tcgatggggt ccccatcaac aacacgacgt tcctcacagg taacaacgac
     1981 ttcggctcca tccaaagctc cgacggcgcc tcagacatca acccggatga catcgagtcg
     2041 atgaccgtcc tccgcggagc ctcggcggca gccctctatg gcagccaggc cggtaatggc
     2101 gtgatcgtca tctccacaaa aaaaggccac aagaatcgca ccgacgttac catcaactcc
     2161 ggcatcggct tcgaacgccc cttcgccctg ccgcaattcc aggatagcta tggccagggc
     2221 aacacagggc tcatcgctac cgactcctcg caaagcggag ccagctgggg agccaaaatg
     2281 accggccaga gttatattga ctacctgtat aaaaggaata cctattcccc ccaaccggac
     2341 aacgtcaaaa agttcttcaa tacagggctt agcctgaaca actcggtagg ggtctccatt
     2401 ggcggcgata aaacccagag ctacttctcc tatacgaaca actacgccaa aggcattacc
     2461 ccaagcaacg acatgaaccg gcatatcttc gactatcgca tcaccagcga tatcaacaag
     2521 aagttctccg tcgatgccaa ggcgacctat atcctccaga tcatcaacgg ccgtccccgt
     2581 acgggcgaag agaacagccc cgtattcgac gtataccagc agccccgcag tatcagctac
     2641 gacgaggcaa agaacaactt ccagaccttc gacgcctttg gagtggccaa acccgccaac
     2701 tgggccggta ccaacagctc catctaccaa agcccctatt ggatgaccaa caacacgtcc
     2761 atcagcgaga accgcgaaag ggcgatcggc ttcgcagcat tgaaatataa tatcaccccc
     2821 tggctgaaca tccgcggctc cgctaacatc gaccgcacct cggacgagat ccagcagaaa
     2881 tat
//
LOCUS       DATGFG010001079         4699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1202262, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001079 DATGFG010000000
VERSION     DATGFG010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202262"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VKQ52_20495"
     CDS             complement(<1..1191)
                     /locus_tag="VKQ52_20495"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HLZ89650.1"
                     /translation="MKQLPGSPLGLDQYTGDWDTTHVVHLLKRTLLGATVQDIGYFKN
                     LGMSAAVDQILQPTPVPTTKPLNNYGSDPTGVAPWQTWIGSGLTYVDTTMNTNRLRSL
                     QDWWIGQLLNCGRSIHEKMTLFWHNHFAPNALPHLDIPCELWYNQYLTLRRYALGNFP
                     QLMKAITLDPAMLLMLNGSTNTKTSPNENFGRELQELYTIGKGPDGTGSGYVQNDVQY
                     AARVLTGHTVDLNYNYLFQAGDHDDTNKSFSAFYGNQIITGYSGAAGAGELDTLLSML
                     FSTQESAKFLCRELYNFFIYYVIDDTIETNIITPLAEVLRQSGYNITTTLSVLFKSRH
                     FYDLVNSGACVIKSPLDMMIGLCREYQVAIPADAAGQYNAWRMLAQNASDLQQEILCI
                     PVVAG"
     gene            complement(1196..1672)
                     /locus_tag="VKQ52_20500"
     CDS             complement(1196..1672)
                     /locus_tag="VKQ52_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89651.1"
                     /translation="MKSHSLLTCLLSFCCILASCGKSSNGPTVAPRSAITFSANDSLI
                     SYTINLVYIQDVDTTHTTLISGEYADTSVRKGSLAIHLPGDTTGRFSGASVLVTYTDG
                     KGAVYNNTADSTNYVQVDKYPKSYNGVVSGSFSFVVTGSAGSITFSNGSFIAIYQK"
     gene            1788..2624
                     /locus_tag="VKQ52_20505"
     CDS             1788..2624
                     /locus_tag="VKQ52_20505"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HLZ89652.1"
                     /translation="MNIFDRFSNGWNIAMNSWAVLKENRRLILFPILSGISLILVILS
                     FVLIAFASVGWDPDALRDVREQPTVVNYLFVFGYYLVNYFIIVFFNTALIHCTHLYFN
                     GEKPTIRDGLQFSLSRIGVLLSWAAFAATVGTALRILQDNLGSIGKFITGLIGIVWSV
                     ATFFVVPVIAYENLGPIAAFKRSASLMKEKWGESLAASFSFGLIQFLGIIVLAGVGFL
                     LGWAIHPIVGIVVFTLGVFAVMVLVSAVRTIFISAVYHNLNGDPVKHFNQQFADHLFM
                     EK"
     gene            2750..4171
                     /gene="miaB"
                     /locus_tag="VKQ52_20510"
     CDS             2750..4171
                     /gene="miaB"
                     /locus_tag="VKQ52_20510"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HLZ89653.1"
                     /translation="MLESLSSKVHDEGRQGEAYAPVAAGTREFRKKFYIESYGCQMNF
                     ADSEIVASILNDSGFGATGNYAEADLVLLNTCSIREKAEQTVRNRLHLFRQLKESRPG
                     MLIGVLGCMAERLKGKFLEEEKLVDIVVGPDAYRTLPGLVEEAESGQKAVNVLLSRDE
                     TYADISPVRLDSNGVTGFVSIMRGCNNMCSFCVVPFTRGRERSRDAVSIVRECTELFD
                     KGYREVTLLGQNVDSYYWMDEVADQAVTFAQLLEKVALIDPSLRVRFSTSHPKDITDE
                     VLQTMARHENICRYIHLPVQSGSTRVLQLMNRTYTREWYLAKIDRVLSVLPDCGLSTD
                     IIAGFCTETEEDHRDTLALMEYARYDMSYMFFYSERPGTLAARRYADDVPEEVKRRRL
                     DEIVKLQNRLSLEKNKADVGKTFTVLIEGDSKKSAADWRGRNSQNKVVVFPKAGAHLR
                     KGDYARVKIEDCTGATLIGRVTE"
     gene            4179..>4699
                     /locus_tag="VKQ52_20515"
     CDS             4179..>4699
                     /locus_tag="VKQ52_20515"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HLZ89654.1"
                     /translation="MEIQSIKNRFGIIGNSPGLNFALQVAVQVAATDLTVLIAGESGV
                     GKEAFSMIIHALSGRKHNPFIAVNCGAIPEGTIDSELFGHEKGSFTGAVDSRKGYFET
                     VNGGTIFLDEIGEMPLGTQARLLRVLEAGEFIRVGSSKVQKTDVRVIAATNKDLLELT
                     EKNKFREDLYYRL"
BASE COUNT          996 a         1180 c         1420 g         1103 t
ORIGIN      
        1 ccccgccact acggggatac agaggatctc ctgttgcagg tcggatgcgt tttgggcgag
       61 catgcgccag gcattgtact ggcccgctgc atcggccggg atcgccacct ggtattcccg
      121 gcaaagcccg atcatcatgt cgagggggct tttgatcaca caggcgcctg aattgacgag
      181 gtcatagaaa tgccggctct tgaagaggac cgacagagtg gtggtgatgt tatagccgct
      241 ctgccgcagg acttcggcca gcggggtaat gatattggtc tcgatcgtgt cgtcgatgac
      301 ataatagatg aagaaattat acagctcccg gcaaaggaat tttgcggatt cctgggtgga
      361 aaaaagcatg gaaagcaggg tatccagttc gccggcgccg gccgccccgg aatagccggt
      421 gatgatctgg ttgccgtaga aggcggagaa ggacttgttg gtgtcgtcat ggtctcccgc
      481 ctggaaaaga taattgtagt tcaggtccac cgtatgtccc gtcaataccc gggccgcgta
      541 ctgcacgtca ttctggacat agccggaccc ggttccatcc gggcctttgc cgatggtgta
      601 gagttcctgc agttcgcgac cgaaattctc attcggcgag gtcttggtat tggtgctgcc
      661 gttcagcatc agcagcatgg cggggtccag cgttatggcc ttcatcagct gcgggaaatt
      721 gccaagggca tacctgcgca gggtaaggta ttggttatac cagagttcgc aggggatatc
      781 gagatgggga agcgcgtttg gcgcaaaatg attgtgccag aacagggtca tcttttcatg
      841 gatcgaccgg ccacagttga gcagttgccc gatccaccag tcctgaagcg aacgaagccg
      901 gttggtgttc atcgtcgtgt ccacatacgt gagcccggaa ccgatccagg tctgccaggg
      961 cgctacgccg gtggggtctg acccgtaatt gttcaatggt ttggtggtgg ggaccggcgt
     1021 gggctgcagg atctggtcca ccgccgcgga catgcccaga tttttgaaat agccgatgtc
     1081 ctgcacggtg gcgcccaaaa gcgttctttt cagcaggtgg accacatggg tagtgtccca
     1141 gtcgccggta tattgatcca gccccagcgg gctgcccgga agttgcttca tggttttatt
     1201 tttgatagat ggcgataaaa ctgccgttcg aaaatgttat gctgccggca ctcccggtta
     1261 ccacgaacga gaaactcccg ctcaccactc cgttatagga tttgggatac ttatccacct
     1321 gcacataatt agttgaatcc gcggtgttat tatacaccgc tccctttcca tccgtatagg
     1381 tgaccagcac gctggcgccg gagaaccggc ccgtcgtatc gccgggcagg tggatggcga
     1441 ggcttccttt tctgacggaa gtgtccgcat actcacccga gataagcgtc gtgtgggtgg
     1501 tgtcgacatc ctggatatag acgaggttga tggtatagct gatgagggaa tcgttggcgc
     1561 tgaaggtgat ggctgatctt ggcgcgaccg tgggtccatt gctgctttta ccgcaggagg
     1621 ccagtataca acagaaggac aacaggcagg ttagcaggga gtggcttttc atagtgttct
     1681 gttttggctg gtccccaccc gttatgcaat tttcaggcca aagggtttat gcaatactgg
     1741 tttttatgct tagattagta atctaattcc tcaccaaacc tctttttatg aacattttcg
     1801 accgtttttc gaatggctgg aacatcgcga tgaacagctg ggccgtgtta aaggagaacc
     1861 gccggctgat cctcttcccg atcctttcag gcatttcgct tatcctggta atcctttctt
     1921 ttgtgctgat cgcctttgct tcggtcggct gggaccccga tgcgctccgg gatgtccggg
     1981 agcagcccac ggtagtgaac tacctgttcg tattcgggta ttacctggtg aactatttta
     2041 tcatcgtctt cttcaatacc gccctgatcc attgtaccca tctctatttt aacggcgaaa
     2101 agcccacgat ccgggatggc ttgcagttca gcctgagccg cattggtgtc ctgttgtcct
     2161 gggctgcttt tgcggctacc gtcggaaccg ccctgcgcat cctgcaggat aacctgggtt
     2221 ccatcggcaa gttcattacg gggctgatcg ggatcgtatg gagcgtggcc accttcttcg
     2281 tcgtaccggt gatcgcctac gagaacctcg ggccgattgc cgcgttcaaa cgttccgcca
     2341 gcctgatgaa agaaaaatgg ggcgaaagcc tggcggccag tttcagcttt gggcttatcc
     2401 agttcctggg catcatcgtg ctggcggggg tcgggttcct gctgggctgg gccatccacc
     2461 ccatcgtggg tatcgtcgta tttaccctcg gggtgttcgc ggtaatggtg ctggtcagcg
     2521 ccgtccggac gatcttcatc agcgccgtgt accataatct gaatggcgac ccggtgaaac
     2581 acttcaacca gcagtttgcc gatcatcttt ttatggagaa ataaattcct ttccggttgc
     2641 aaaacccggg gccgtcgcca tggcgacggc tttttttcgc ccccatctgc caacttgtca
     2701 ttgctaaaag gtttctacgt atctttgccg tctaaatcac aaaagaccca tgcttgaatc
     2761 gttgagtagc aaggttcacg atgaggggcg gcagggtgaa gcttacgctc ccgtggctgc
     2821 cggtacgcgg gagttccgga agaaattcta tatagaaagt tatggttgtc agatgaattt
     2881 cgcggacagc gagatcgttg cctccatttt gaacgacagt ggatttgggg ctacgggcaa
     2941 ctatgccgag gccgacctgg tattgttgaa cacctgttcc atccgcgaaa aggcggagca
     3001 gacggtgcgc aaccggctcc acctgttccg ccagctgaag gagagccggc cggggatgct
     3061 gatcggggta ttgggttgta tggccgagcg gttgaaggga aaattcctgg aagaagagaa
     3121 gctggtggat atcgtggtcg ggccggatgc ctaccgtacc cttccgggcc tggtcgagga
     3181 agccgagtcg gggcaaaagg ccgtcaatgt ccttttgagc cgtgacgaaa cctatgcaga
     3241 catttcccct gtgcggctgg acagcaatgg ggtgacgggg ttcgtgagca tcatgcgcgg
     3301 ctgcaacaat atgtgcagtt tctgtgtggt tccctttacg cggggccggg aacgcagccg
     3361 ggacgcggtg tcgatcgtcc gggaatgtac cgaactgttt gataaaggat atagggaagt
     3421 gacccttttg ggtcagaacg tggatagcta ctactggatg gacgaggtcg ccgatcaggc
     3481 cgtgaccttt gcccagctgc tggaaaaagt agcccttatc gatccctcgc tgcgggtgcg
     3541 tttttcaacc tcccatccca aggacattac cgatgaggta ttgcagacca tggcgcgcca
     3601 tgagaatatc tgccggtata tccatttgcc ggtgcagagc ggcagcaccc gggtcctgca
     3661 attgatgaac cggacctata caagggaatg gtatctggcg aagatcgaca gggtcctgtc
     3721 ggtactcccc gactgtgggc tgagcacgga catcattgcc ggcttttgta ccgagacgga
     3781 ggaggatcat cgcgatacgc tcgccctgat ggaatatgcg cgttatgata tgagctatat
     3841 gttcttttat agcgagcggc ccggaacgct ggcggcacgt cgctatgcgg atgatgtgcc
     3901 cgaggaggtg aagaggcgaa ggctggatga gatcgtgaag ctgcaaaacc ggctctcgct
     3961 cgagaagaac aaggcggatg tgggaaagac cttcaccgtg cttatcgagg gagactcgaa
     4021 gaagagcgcc gccgactggc ggggacgcaa cagtcagaac aaggtggttg tattccccaa
     4081 ggccggcgcg catctccgga agggcgatta cgcacgggtg aaaattgaag attgtaccgg
     4141 ggcgacgctg atcggccggg taactgaata aaatttgtat ggaaattcaa tcaataaaga
     4201 accggtttgg catcatcggg aattcgcccg ggttgaactt tgcattgcag gtagcggtgc
     4261 aggtggcggc gacggatctc acggtgctta tcgccggaga aagtggggtt gggaaagagg
     4321 cattttcgat gatcatacac gccttgtccg gccgtaaaca taatcccttc attgccgtca
     4381 actgcggggc cattcccgaa ggcacgatcg attccgaact cttcgggcac gaaaagggct
     4441 cctttacggg tgcggtggat tcgcgcaaag gatatttcga aacggtgaac ggcgggacca
     4501 tcttcctgga cgagatcggg gagatgcccc tgggaactca ggcaaggctg ttgcgggtac
     4561 tcgaggccgg ggaattcatc cgggtgggct cgtccaaggt acaaaagacg gatgtccggg
     4621 tgatcgccgc aacgaacaag gacctgctcg aactgacgga gaagaacaag tttcgcgaag
     4681 acctttatta ccgcctgag
//
LOCUS       DATGFG010001080         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_404042, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001080 DATGFG010000000
VERSION     DATGFG010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404042"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1501)
                     /locus_tag="VKQ52_20520"
     CDS             complement(20..1501)
                     /locus_tag="VKQ52_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89655.1"
                     /translation="MFYCAFGTPPVFCDVNRIADDGFGKTYKAMKYTILILLLVSATM
                     ATARAQFLKKIVSKALSGGGNKDSANHPQHHGKDNPLDNILNRTGSSGRDRDSSLILA
                     KDDDVKATHGDLSRFNARPYYTLLEGEKFTFYEACLLMQADQLNPRILVVKDRKPWLI
                     GANGEHIAVDPRKVSACPLNSRRTNMALLASLNGDQSEPYARQPSIFAVRSPGGGAVA
                     NELGFDTAALAGMQKSLDAGDSAAAFRQAMTMQKPVANEAMAFKPRTTIVFRGKTFGP
                     YMEVRSYVFDRGAGKFLAVVDLRRPNTDITTIKGDFSRLVEPWLIGSDGTKVKLPDGE
                     ARIVADENLAFVRIYIVKRASGDGGVVHASLFDPVAHLNTPVFTNEDGSGNPGITQNI
                     DIPTGNLVTKQLDGQRIKVYINDKCVTTLDGESDIPFPNVFLNKDASRWAIWGTRALY
                     FSNGETVDGVIAVRVADRSGRTSLNWIALGSDNKTLQICHCEL"
     gene            1561..2172
                     /locus_tag="VKQ52_20525"
     CDS             1561..2172
                     /locus_tag="VKQ52_20525"
                     /inference="COORDINATES: protein motif:HMM:NF013972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HLZ89656.1"
                     /translation="MRVIPICCLFLRLVFFDSGANDWRLKDQKDGISVYSRRSEFSKF
                     NDIEVEMDLPGTVAQLTSILLDVEKYPQWAYCTKSSTLIKKIGSNEVIYYSEISAPWP
                     ISNRDFYADIVLCCDSSKHSSCLVSSGLKDFLPEKRGLVRIPRSKAVWNISQKSDKTI
                     HLQYILQVDPGGCLPAWVANMFASKGPLETFASLKKKMEALNP"
     gene            complement(2206..2847)
                     /locus_tag="VKQ52_20530"
     CDS             complement(2206..2847)
                     /locus_tag="VKQ52_20530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89657.1"
                     /translation="MERPDLIFVTGCNAAGKTSLIRSHLSEFPDYEIIMTDVYKGRSR
                     EVFREAVRTNQNILLETPFNDEGFRDLIDLAKLAHYQSSLVVLFLKSPAQSIERVANR
                     TVLENGLHISSGNVEHNFMENLKNVAKYFLYFDESHFYYTGERGHNRLIMRYEKDKLA
                     EYVKNDFAYVQHFANLAYRFERLATEDKNIIVANKDYRVGVRNSPVGKGHKLH"
     gene            complement(2847..3101)
                     /locus_tag="VKQ52_20535"
     CDS             complement(2847..3101)
                     /locus_tag="VKQ52_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89658.1"
                     /translation="MNPRVQQLLTNMDVLPELGRQRCFELGNPFYYQDAVDAMEPDNE
                     SANYWRKELPTGELFLVTLQVSEVEGKTYSIKDTVIRQLR"
BASE COUNT          759 a          794 c          849 g          849 t
ORIGIN      
        1 aaggacgaag aggagccgtc tatagttcac aatgacagat ctggagggtc ttattatcgc
       61 ttcccagggc gatccagtta agcgaggtcc tgccgcttcg atccgcgacc cggaccgcga
      121 tgacgccgtc caccgtttcg ccatttgaaa aatacagggc cctggttccc cagatggccc
      181 atcgggaagc gtccttgttt aaaaacacat tggggaaagg aatatccgat tctccgtcga
      241 gggtcgtcac gcatttatcg ttgatgtata ctttgatgcg ctgaccgtcg agctgtttcg
      301 tgacaaggtt gcccgtcggg atatcgatgt tctgcgttat tcccggattg cccgacccat
      361 cctcattggt aaagacgggg gtatttagat gcgccacggg atcgaacagg gacgcgtgaa
      421 caaccccacc atcgccggag gctcttttca cgatatagat gcgtacgaat gcaaggtttt
      481 catcggcgac gatgcgggcc tcgccatcgg gcaatttcac cttcgtgccg tccgaaccga
      541 tgagccaggg ttctaccagc ctgctaaaat caccctttat cgtggtaatg tccgtattgg
      601 gccgccgtag gtccacgaca gcgagaaatt tgccggctcc cctgtcgaag acgtaagatc
      661 gaacttccat ataggggccg aaggtctttc cccggaagac gatggtagtc ctgggcttga
      721 aggccatggc ctcattggcg accggtttct gcatggtcat tgcctgccgg aaggccgcgg
      781 cggagtcgcc ggcatcgagg gatttttgca tgccggccag cgcagccgta tcaaaaccga
      841 gctcattcgc caccgccccg ccgccgggag accgcacggc gaagatggac ggctgcctgg
      901 cgtagggctc ggactggtct ccgttgaggc tggcaaggag ggccatattg gtgcggcggc
      961 tgttgagcgg acaggcgctc acctttcggg gatccaccgc gatgtgttca ccattggcgc
     1021 cgatgagcca gggcttgcga tcttttacca ccaggatccg ggggttcagt tggtcggcct
     1081 gcatgagcag acaggcttca taaaaagtga atttttctcc ctccaacagg gtataataag
     1141 ggcgggcgtt gaatctcgag aggtcgccat gcgtcgcttt cacgtcgtcg tccttggcaa
     1201 gtataaggga agaatccctg tcccttccac tgctgcctgt cctgttcagg atattatcca
     1261 atggattgtc tttgccgtgg tgctggggat ggttggcgct gtctttgttg ccgccgccgg
     1321 aaagcgcctt tgaaactatc tttttcagga actgggccct tgcggttgcc atggtagcgg
     1381 aaacaagcag gaggataagt atggtgtatt tcatcgcctt ataggttttg ccgaagccat
     1441 cgtcggctat tcggttgacg tcgcaaaata caggcggcgt cccgaaggcg caatagaaca
     1501 ttcgttaggg cgttatattc gttatattgt ggtgattagt gttgattagt actgattagt
     1561 atgagagtga tcccgatctg ttgtttattt ttaaggcttg tcttctttga ttccggcgcc
     1621 aacgactggc gactaaaaga tcagaaggac ggcatatccg tctacagccg ccggtcagaa
     1681 ttctccaaat tcaatgatat tgaagtagaa atggatcttc cgggaacagt agcccagctc
     1741 acttccattt tgcttgatgt cgaaaaatat ccgcaatggg cttattgcac caagtcctcg
     1801 accctgataa agaagatcgg cagcaacgaa gtcatttatt attccgagat cagcgctccc
     1861 tggccaataa gcaaccggga tttttacgcc gatatagtgc tgtgttgtga cagcagtaag
     1921 cattcctcgt gcctggtttc gagtggattg aaagattttc tcccggaaaa aaggggcctg
     1981 gtgcggatcc cacgctcgaa agcggtctgg aacatctcgc aaaaatccga caagaccatc
     2041 catttacaat atatcctgca agtagacccc gggggctgcc tccccgcatg ggtcgccaat
     2101 atgtttgctt caaaaggccc gctggaaaca ttcgccagcc tgaaaaaaaa gatggaagca
     2161 ctgaatccct aatcccattg gctgataggc gggtttttta ttgaatcagt gcagtttgtg
     2221 ccccttgccg accggggaat tgcgaacgcc aactctatag tccttgttgg caactataat
     2281 attcttatct tccgtggcaa gcctttcaaa gcgataggcc agatttgcaa aatgctggac
     2341 ataggcaaag tcatttttga catactctgc tagtttgtct ttttcatacc gcatgatgag
     2401 acgattgtgt ccccgctccc ccgtgtaata aaaatgggat tcgtcgaagt agaggaaata
     2461 tttggcgacg ttttttagat tctccatgaa attgtgctct acgtttcccg aggaaatgtg
     2521 aagaccgttt tcgaggactg ttctgttagc aacgcgctca attgattgtg ccgggctttt
     2581 gagaaaaagt accaccaggg aggattgata gtgggccaat tttgccaaat caattaaatc
     2641 tctaaatccc tcgtcattaa agggcgtttc aaggagaata ttttgatttg tccggacagc
     2701 ttcacggaag acctcgcgcg atctgccttt gtaaacatcg gtcatgatga tctcgtagtc
     2761 gggaaactcg ctcaaatggc ttcttatcag gcttgttttg ccagctgcgt tgcaccccgt
     2821 aacaaaaatg agatcgggtc tttccattat ctaagctgtc tgattacggt atccttgata
     2881 gaataagttt tcccttccac ttcactgacc tgcaaggtca ccaaaaatag ttctccggtg
     2941 gggagttcct ttcgccaata gttggccgac tcgttgtccg gctccatggc atcaacggcg
     3001 tcctggtaat agaagggatt tcccagttcg aagcatcgtt gcctgcccaa ttcgggcagc
     3061 acatccatat tggttaaaag ctgttgaacg cgaggattca tatgctaaag ataccgtttt
     3121 ttcacccata tcgtttagtt aagcaaaaaa aggtgatttc acggtgcagg atcgacgaat
     3181 tattttgaat cgcatagtta gaaggggtca ttcgtccgta aaaaggcaaa ccaggattat
     3241 cgggtcaaat t
//
LOCUS       DATGFG010001081         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1086441, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001081 DATGFG010000000
VERSION     DATGFG010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086441"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VKQ52_20540"
     CDS             complement(<1..619)
                     /locus_tag="VKQ52_20540"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HLZ89659.1"
                     /translation="MQLQPVDTVDSISADDFKEHYYTPMKPLVIRGLSRHWPALEKWD
                     WDYFKSIVGEERVGVYNNVKSDAYTPINTADDYMKFGEYIDMVRKGPAEWRIFLFNIF
                     DHAPQLVRDFEWPEQLMKGFVKRYPMLFVGGRGSVTHMHFDIDLSHIVHTQFIGRKRV
                     LLFPFEEQHKIYRKPWEVLSFVNFEKYFDAEENKLELEKYPAVRLA"
     gene            720..1421
                     /locus_tag="VKQ52_20545"
     CDS             720..1421
                     /locus_tag="VKQ52_20545"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HLZ89660.1"
                     /translation="MLLHLHPVNPQPRNIKMVIECLLDGGVIIYPTDTIYGLGCDIFQ
                     HKAVERICRIKQVDPAKAQLSFICYDLSDLSNYTKSISTPLYRLLKTYLPGPYTFILP
                     ASKLVPKILKSRKDTIGLRIPDNIIARSLIRELQHPILSSSLPGPFAAHGPDENIAHG
                     RKGSNDGPSAAPPAPWVEEYTDPEMIREKFENQVDIIIDGGIGGMVPSTVVDCTGDEP
                     VLIRKGLGEWEAVNP"
     gene            complement(1411..2034)
                     /locus_tag="VKQ52_20550"
     CDS             complement(1411..2034)
                     /locus_tag="VKQ52_20550"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HLZ89661.1"
                     /translation="MENPLRSHYQEELTEAGCDEAGRGCYAGPVFAAAVILPPGFFHP
                     LLNDSKQVTPENRNELRVFIEANALAWAVAKVDHDEIDRINILKASFKAMHLALDQLK
                     PRPRLLLIDGNRFTKYKRTPHRCIIKGDSIFASIAAASILAKTWRDEYMQQLHREYPH
                     YSWDSNKGYGTKEHRDAIERFGLCKYHRKSFNISPAADPQEELEFMD"
     gene            2105..3232
                     /locus_tag="VKQ52_20555"
     CDS             2105..3232
                     /locus_tag="VKQ52_20555"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HLZ89662.1"
                     /translation="MQSSSRSSSRPVAIWVYTGVIMLLIQVVLGGITRLTGSGLSITE
                     WKVVTGSLPPLNAAQWQDAFDKYRQTPQFRLINPDFTLADFKFIFFWEWFHRFWARLV
                     GVVFLVGFGWLLWKKKLQPAMIRPLIILFLLGALQGAIGWIMVASGLTGDAIYVAPTR
                     LGLHFVFALGLIVYAFWFALQLSVGTEVRLLRRRAGSSRPAEILLLQVTTLRRWTCVI
                     LGLLFFQLLYGALMAGHKAAAVAPTWPTINGSWVPDGLFSQHPLLQDLAGNTITVQFI
                     HRMLAYILIIAILVWTALALRIQGAPDSFRRLRRLPLLVVAIQVLLGICSLLTSPGII
                     AHQWVAFDWLALFHQMTGLLFLLTMVGMLYLVIPVESSVTV"
     gene            3229..>3717
                     /locus_tag="VKQ52_20560"
     CDS             3229..>3717
                     /locus_tag="VKQ52_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89663.1"
                     /translation="MMRVLKTIYYFFPVQLLLLHVKKFQVLLIFWFILFSTLNGGFMR
                     TYGAAALFLSPEYLGDVSPISTAIVGVATGIFIMSWNITTFIIFCRHFRFLATTTKPF
                     LKYCINNAILPIIFLIFYFIKAFQFSVDRELLPIRSIILLALGFLAGFSFLIIISLYY
                     FFR"
BASE COUNT          725 a         1025 c          984 g          983 t
ORIGIN      
        1 tggcgagccg cacggcggga tatttttcca gctccagctt attctcttcg gcatcgaagt
       61 atttttcgaa attgacgaag ctcagcactt cccagggttt gcgatagatc ttgtgttgtt
      121 cttcgaaagg gaagagcaat acccttttgc ggccgatgaa ctgggtatgg acgatatgcg
      181 agagatcgat gtcgaaatgc atgtgggtca cggagcccct cccgcccacg aacagcatgg
      241 gatagcgttt gacaaagcct ttcatgagct gttcgggcca ttcgaaatcc cggaccagct
      301 gcggggcgtg atcgaagata ttgaacagga agattcgcca ttccgcggga cccttgcgta
      361 ccatatcgat gtattcgccg aacttcatat agtcatcggc ggtattgatc ggcgtatagg
      421 catcgctttt gacattgttg tataccccga ccctttcttc ccccacgatg gatttgaaat
      481 aatcccagtc ccacttttcc aatgcaggcc aatgtctgga caggccccgg atcaccaacg
      541 gcttcatggg agtatagtag tgctccttaa aatcgtcggc acttatactg tccacggtat
      601 ctaccggttg taattgcatg ctagacatta ttttcgcaaa tttaaaaata attctatgtt
      661 aaagaattgt taggaaaaaa ccaccggtag ctttcccgcg atttttccta aattacccta
      721 tgttattgca tttgcacccg gtgaatcccc agcccaggaa tataaagatg gtgatcgaat
      781 gtttgcttga tggcggggtg atcatttatc ctaccgatac gatctatggc ttggggtgtg
      841 atattttcca gcataaggcc gtcgaacgca tctgccggat caaacaggta gaccccgcca
      901 aggctcaact ctcgttcata tgttatgatc tcagcgatct cagcaattat acaaaaagca
      961 tttcaacccc cctgtaccgc ttgctaaaaa cctacctgcc cgggccttat acttttatcc
     1021 ttccggcgag caaactggtt cccaagatac tgaaaagccg gaaagacacc atcggtcttc
     1081 gcatccccga taatattatt gcccggagcc tgatcaggga actacaacac ccgatcctga
     1141 gctcctccct tcccggaccc tttgctgccc atgggcccga tgaaaatata gcccatggcc
     1201 gcaaaggatc taatgatggg ccttcggccg cacctccggc gccttgggtc gaagagtata
     1261 ccgatcctga aatgatccgg gaaaaattcg agaaccaggt ggacatcatc atcgatgggg
     1321 gtatcggggg aatggtccct tcaacggtgg tggattgtac gggtgacgag cccgtcctga
     1381 tcaggaaggg attgggggag tgggaggccg tcaatccatg aattccagct cttcctgggg
     1441 gtctgcggcg ggggagatat taaagctttt gcggtggtat ttgcaaagcc cgaatctttc
     1501 gatggcgtcc cggtgctcct ttgttccata gcccttgttg ctatcccagc tgtaatgggg
     1561 gtattcccga tgtagttgtt gcatgtattc atcgcgccag gtcttggcga ggatgctggc
     1621 cgcggcgatg gaggcgaaga tggagtcgcc ctttatgatg cagcggtggg gcgtgcgttt
     1681 atactttgta aagcggttgc cgtcgatgag caggaggcgg ggcctgggtt tcagctggtc
     1741 gagggcaagg tgcatcgcct tgaaggaggc tttcaggata ttgatccggt cgatctcgtc
     1801 atgatccact ttcgctacgg cccaggccag ggcattggcc tcgataaaga cgcgcagttc
     1861 attacggttc tccggcgtca cctgtttgga gtcgtttagc aggggatgga agaaccccgg
     1921 ggggaggatg accgctgccg cgaataccgg tccggcatag caaccgcgcc cggcttcatc
     1981 acagccggct tcggtcagct cttcctggta atggcttctc agcggattct ccatttgcca
     2041 aaaatacgat ccttgacggt tgcctgtccc gatctggata aaaatcgaga actttgcacc
     2101 cgctatgcaa tcctcttccc gttcttcctc ccgcccggta gctatctggg tctataccgg
     2161 ggtaatcatg ttgctcatcc aggtcgttct tggcggtatc acccggctta ccggatcggg
     2221 gctttccatc accgaatgga aggtggtcac cggcagcctg ccgccgttga acgccgcgca
     2281 gtggcaggat gcctttgaca aataccggca gaccccacag tttcggctga tcaacccgga
     2341 ttttaccctt gccgacttca aattcatttt tttctgggag tggttccacc gtttctgggc
     2401 ccgccttgtc ggggtagtgt tcctggtcgg gttcgggtgg cttttatgga agaagaagct
     2461 gcaaccggcg atgatccggc ccctgatcat cctgttcctg ctgggcgcct tacagggggc
     2521 catcggctgg atcatggtgg ccagcgggct caccggtgat gcgatctatg tggcgcctac
     2581 ccggctgggt cttcactttg tctttgccct cgggctcatc gtttatgcct tttggtttgc
     2641 gcttcaattg tccgttggca ccgaagtccg ccttctccgc cggcgtgcgg ggtcttcccg
     2701 gcccgcggag atcctgctgc tgcaggttac cactctccgg cgctggacat gcgttatcct
     2761 cggcctgctt tttttccagt tgttgtatgg ggccctgatg gccggacaca aggccgcggc
     2821 cgtggcgccc acctggccca cgatcaacgg cagctgggtg cccgacggac tgttcagcca
     2881 gcatccgctg ctccaggacc ttgccggcaa cacgatcacg gtgcagttca tccaccgcat
     2941 gcttgcctat atcctcatta tcgccatcct ggtctggacg gcgcttgcgc tgcggatcca
     3001 gggggcgccg gactcttttc gccggctgcg ccggctgccc ctgctggtcg ttgccatcca
     3061 ggtcctcctg ggcatttgca gcctgcttac cagtcccggg atcatcgccc accagtgggt
     3121 ggcctttgac tggctggcgc tgttccacca gatgacgggg ctcttattcc tgctgacaat
     3181 ggtggggatg ttgtaccttg tgatacctgt agaaagttct gtaaccgtat gatgcgagtc
     3241 cttaaaacca tttactattt ctttcccgta cagcttcttt tgttgcatgt aaaaaagttc
     3301 caggtactgc tgatattctg gttcatcctt ttcagcaccc tgaacggggg atttatgcgg
     3361 acttatgggg cggcggcgct ctttctttcg ccggaatatc tgggggatgt cagcccgata
     3421 agcactgcca tcgtgggggt ggcgaccggc atctttatca tgagctggaa catcacgacc
     3481 tttatcattt tctgccggca tttcagattc ctggcgacga cgacgaagcc ctttctcaaa
     3541 tactgtatca acaacgccat cctccccatc attttcctta tcttctattt tataaaggcc
     3601 tttcagttca gcgtcgaccg cgaactcttg cccatccgct ccatcatcct gctcgcttta
     3661 ggtttcctcg cgggtttcag tttcctgatc attatatccc tctactattt ctttcgc
//
LOCUS       DATGFG010001082         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_404280, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001082 DATGFG010000000
VERSION     DATGFG010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404280"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VKQ52_20565"
     CDS             <1..1270
                     /locus_tag="VKQ52_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89664.1"
                     /translation="AAPLKGTIKGTMLAGKTYTIGGDVFINQGDSLIIQEGVTINVTG
                     KYGIGVNGSLICLGTQAKPNRITFPGLTKTDQEGADPNKDSAFIGKWIGIIGGINCPM
                     MIFKWTHIEYGGGAIPATSGISQIFASPYPLFFQNPAGIFVLEDSWVYGSTDDPFRIL
                     GGKIAVMRNTFEKCGYTGGEAMNAKAGTIGDFAYNLCVGMATNGPKLSNSGAAVGVPA
                     SNVRMYNNTIINCGYRRAASGRGGSIDFEQGAGGMAYNNIIVNCKFGLRVVQNPIADT
                     ANLRYGNNWTYADSLSVANNIYPSMAVSVCITKAISTDIPTPSALLPAGWQPGNSYTA
                     PASVLGANNPQFINGPVPMPAGLNLRDISAVGNYNFRLQSGSPCIGKGYTGFSPRGDV
                     PVDPRYGATEITPPGKDLGAYQYNGTGNQH"
     gene            1272..>2413
                     /locus_tag="VKQ52_20570"
     CDS             1272..>2413
                     /locus_tag="VKQ52_20570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLZ89665.1"
                     /translation="MKIIFAFLALLLTLGGHAQDANRRTQDANGRTRDANFIAPPYLQ
                     IGRTPSPGSLDLLWQTKDAPGEWKVEWKSATGGKWQTLPPATVTRVAPAGLAPRLLYK
                     TSFTGLTPGALFDYRVRRDGRIIFTAAAHALKAADQSFRLIVSGDIGAATPDAKLLAQ
                     QAWLAKPDLFVIPGDIIYENGRVSEYDTNFWPVYNADSSTPSGAPLMRSIAFVGAPGN
                     HDLDNRSLDQFPDGLAYFYFWDQPLNGPEGREGNAFVPPLKASDSNRAAFIRAAGDAY
                     PRMANYSFDYGNTHWTVIDSDPYVDWTDKGLCDWVAKDLAAATQATWRFVVFHHPGFN
                     SSREHYEQQQMRLLSPIFEKGSVDIVFCGHVHNYQRSYPLTFLPDR"
BASE COUNT          523 a          802 c          652 g          436 t
ORIGIN      
        1 tgcggccccg ctgaaaggca ccatcaaggg gacaatgctg gccggcaaga cctataccat
       61 cggcggcgac gtcttcatca accagggaga ctcgctcatc atccaggaag gagtgaccat
      121 caacgttaca ggcaaatatg gcatcggcgt gaacggctcc ctgatctgcc tgggcaccca
      181 ggccaaacca aacaggatca cctttcccgg gctgacaaag acggaccagg aaggcgccga
      241 tccgaataag gactccgcct ttatcgggaa atggatcggc atcatcggcg gcatcaactg
      301 cccgatgatg atcttcaaat ggacacacat cgaatatggc ggcggcgcca tccccgccac
      361 cagcggcatc agccagatct ttgccagccc ctatcccctc ttcttccaga accccgcggg
      421 catcttcgta ctggaggata gctgggtcta cggcagcacc gacgatccct tccgcatcct
      481 cggcggcaag atcgccgtca tgcggaacac attcgagaaa tgtggctaca ccggtggaga
      541 agccatgaat gccaaggccg gcaccatcgg cgactttgcc tataacctct gtgtaggcat
      601 ggcgacaaac ggtccaaaac tctccaactc cggcgcggcc gtaggagtac cggcctcgaa
      661 cgtgcggatg tataacaaca ccatcatcaa ctgcggttac cgcagggccg catcgggcag
      721 gggcggctcc atcgatttcg aacagggcgc cggcggcatg gcctataaca atatcatcgt
      781 caactgtaag tttgggctcc gcgtcgtcca gaaccccatc gcggataccg ccaatctacg
      841 gtacgggaat aactggacct atgcagactc cctctccgta gccaataaca tctatccctc
      901 catggccgtg agcgtctgca tcacaaaggc tatatcgacg gatatcccca ccccttcggc
      961 gctgctgccc gccggctggc aacccgggaa ctcatataca gcgcctgctt ccgttctcgg
     1021 cgccaataac ccgcaattca tcaacggccc cgtgccgatg ccggcgggtc tcaatctgcg
     1081 ggatatctcc gccgtgggca attataactt ccgcctgcaa agcggttctc cctgtatcgg
     1141 caagggctat accggctttt ctccaagggg cgacgtaccg gtggacccaa gatatggtgc
     1201 tacggagatc accccgcccg gcaaggatct gggtgcgtat cagtataacg gaacaggaaa
     1261 tcaacattga catgaaaata atttttgcct ttttagccct cttgctcacc ctcggcggcc
     1321 acgcgcagga cgccaaccgc cggacccagg atgccaatgg ccggacccgg gacgccaact
     1381 tcatcgcccc tccttatctg cagataggca ggacgccttc ccccggcagc ctcgatctgc
     1441 tctggcagac aaaagacgcg ccgggggagt ggaaggtgga gtggaaaagc gccaccggtg
     1501 gaaaatggca gacccttccg ccggcaacgg tgacccgcgt ggctccggcg ggcctggcgc
     1561 cccggttgct ctacaagacc agcttcacgg ggttgactcc cggcgccctc ttcgactacc
     1621 gggttcgccg ggacggccgc atcatcttta ccgccgcggc acacgccctg aaggccgcgg
     1681 accagtcctt ccgattgatc gtctccggcg atatcggggc cgcaaccccc gatgccaaac
     1741 tgctggcgca acaggcctgg ctggcaaagc ccgatctgtt cgtcatcccc ggggatatca
     1801 tctacgagaa cggcagggtc tcggaatacg ataccaattt ctggccggtg tataacgccg
     1861 acagctccac gccgtcgggc gcccccctga tgcgcagcat cgcctttgtc ggcgccccgg
     1921 ggaaccacga cctcgacaac cgaagcctcg atcaattccc cgatggcctg gcctatttct
     1981 atttctggga ccagccgctg aacggccccg agggcaggga gggcaacgcc tttgtcccgc
     2041 cgctgaaagc atcggatagc aaccgcgccg ccttcatcag ggccgccggc gatgcctatc
     2101 cccgcatggc caactattcc ttcgactatg gcaatacaca ctggacggtg atcgactcag
     2161 atccctatgt ggattggacc gacaaaggac tctgcgattg ggtggcaaaa gacctggccg
     2221 cggccacaca ggccacctgg cgctttgtcg tcttccacca ccccggcttc aacagcagcc
     2281 gggaacacta tgaacaacaa caaatgcggc tgctctcccc catcttcgag aaggggagcg
     2341 tcgacatcgt gttctgcggg catgtgcaca attaccagcg cagctatccg ctcaccttcc
     2401 tgcccgacag gaa
//
LOCUS       DATGFG010001083         3271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_520837, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001083 DATGFG010000000
VERSION     DATGFG010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520837"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VKQ52_20575"
     CDS             complement(<1..427)
                     /locus_tag="VKQ52_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89666.1"
                     /translation="MYSIKVVLWCLAILTTLSGTLPPRLTTSSAPDLPARPFPQHTVY
                     QVGTILPSHISRQVMDDSVRSFYRVWKKHYILPGCTEGEAYVWFEGTRGSNICVSEGQ
                     GYGMMIVALMAGFDSTAQETYDALYRFYKSHPSQSSPHLM"
     gene            716..1744
                     /locus_tag="VKQ52_20580"
     CDS             716..1744
                     /locus_tag="VKQ52_20580"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HLZ89667.1"
                     /translation="MKTSAFFVLPGLFLYSLISCTHTGLPATQLGNWLASAPIGDPPR
                     GYASVFVIGTDAYVGCGYNQSVGGTGRLKDFWRFNVDSGWAQVADFAGAPRSEAAAFS
                     VGSYGYVGTGYDLVNVYSDFYQYDPVQNVWTRKADFPGTPRYDAVGFGVRGKGYIGTG
                     YNINWFNDFYEYDPAQDKWSVTPGTSGDFQKRQGAVAFVYKDQAYIVTGVNSGGMVRD
                     FWRFDPAQSPAWHQLANITNTDQGTFDDGYTDIERSFATAFVNGDQAYLTTGANVSNI
                     NSTWAYDFASDRWSRRTPWQRASRSGAISFTVSARSFVGTGNSGTNGTFDEFDEFQPT
                     QAFNSNDY"
     gene            complement(1751..>3271)
                     /locus_tag="VKQ52_20585"
     CDS             complement(1751..>3271)
                     /locus_tag="VKQ52_20585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ89668.1"
                     /translation="VYKTLSKKFILPLILLLAANGRISAQACATAQGNQTTYGTNNVW
                     IGYVYTGMNFNNYQGYVNEGSASSPNFDESFGGNQVNYPTNGCSVYTDGFSVRYKLTQ
                     ALSGNYTITVGGDDGYRLSLDGGSTWAINNWNDHGYTTTSYTVTLSGSTNLVLEYYEN
                     GGANRVTFSIVQNCTGTGDPTVYGTGTTWIGYLYQGMNFGTYKGQVTEGSASGPNFDE
                     NFNNPGGSNTNTYNTNSCSVQTYQFSARYRLTQNLAAGSYVFTVGGDDGYRFSLDGGN
                     TWAINNWNDHSYTTSTYSAALSGTTNMVLEYYQNGGYDRISFVQTSTTLPVTLTSWSG
                     SARDNNQALLKWTATNAVDFDHFVVQRSTDGSSFEDIHTIPANDTTEQSYSYTDQDTY
                     DGKLYYRLEMVDRDGKTNYSTIISISLKEAQGIRIYPTRVESGSFFIESSASIRQARL
                     ELFDINGRRLQENEWAALEGRQQVTVNNNASLPAGAYIVRLSANQTTLAKQIIIIK"
BASE COUNT          654 a          797 c         1025 g          795 t
ORIGIN      
        1 ccatgaggtg ggggctggac tggctgggat gggatttata aaagcggtag agggcgtcgt
       61 aggtctcctg cgcggtggag tcgaatccgg ccatgagcgc cacgatcatc atcccatagc
      121 cctgaccctc agatacacag atattggagc ccctggttcc ctcgaaccag acataggcct
      181 ccccttcggt gcagcccgga aggatatagt gctttttcca gacccggtaa aaggaccgga
      241 cgctgtcgtc cattacctgc cggctgatgt ggctggggag aatggtcccc acctggtaaa
      301 cggtatgttg cggaaagggc cgggcgggaa ggtccggcgc ggaggacgta gtcaatcggg
      361 gaggcagggt accggacaac gtggtcagta tagccagaca ccacaaaaca acttttatac
      421 tgtacatggg aaatggaatt gtcaatcatt ccttaaacaa ttaatgtgcc gaagaattcc
      481 cgggggtgaa ggatcagcac ggatgcgtaa tcaacgattc gttaacaatt catataggaa
      541 acattaacag cggcggtgca tcgggatgga cggcccggtg ctcttaccat cggatcttcc
      601 ggaggatatc cctttttttg tgtcttgtat ataacgttta tggatatgcg ctcataatat
      661 gcgcgcaggg atccctatgc ttccactcat gcctcaaaca cttaaaacct ataacatgaa
      721 aacttctgct ttttttgtat tgcccggact gttcctgtat tctcttatct cctgtacgca
      781 caccggtctg ccggctacgc agctggggaa ctggctggcg tcggcgccga tcggtgatcc
      841 tccgcgcggc tatgcttctg tttttgtgat cgggacggat gcctatgtcg ggtgtggtta
      901 taatcaatcc gtcggtggta cgggccgtct gaaggatttc tggcggttca atgtcgacag
      961 cggctgggcg caggtagccg attttgccgg tgcacccagg agcgaggccg cggcctttag
     1021 tgtggggagc tatggctatg tcggcaccgg ttatgacctc gtcaatgtgt acagtgattt
     1081 ttaccagtat gacccggtgc agaatgtctg gacgcggaag gcggattttc ccggtacgcc
     1141 gcggtatgac gcggtcggct ttggcgtacg gggcaagggg tatatcggca ccggctacaa
     1201 catcaactgg tttaatgatt tttacgagta cgacccggct caggacaaat ggtcggtaac
     1261 gcccggcacc agcggtgact tccagaagcg ccagggcgcg gtagcctttg tttataagga
     1321 tcaggcctat atcgtcacgg gggtcaacag cggggggatg gtccgggatt tctggcgctt
     1381 tgatcctgcg caaagtccgg cctggcatca attggccaat atcaccaaca cggaccaggg
     1441 tacattcgat gatgggtata cggacatcga acggtcgttt gccacggcct tcgtcaatgg
     1501 ggaccaggct tatcttacta cgggggccaa cgtcagcaat atcaattcca cctgggccta
     1561 tgattttgcc agtgatcgct ggagccggcg gacgccgtgg cagcgggctt cgagaagcgg
     1621 cgccatctcc ttcacggtga gcgcaaggag ttttgtgggc accggcaaca gcggcaccaa
     1681 cggcaccttt gatgagttcg atgagttcca gccgacacag gccttcaatt cgaatgacta
     1741 ttagggtgat ctatttaatg ataatgatct gtttggccag ggtggtctga ttggccgaca
     1801 gccggacgat ataggctcct gcgggcaggg aggcgttgtt gttgacggtc acctgctggc
     1861 ggccttcgag tgcggcccac tcgttctcct gtaatctgcg cccgttgata tcgaacaatt
     1921 cgagccttgc ctggcggatg gaagcggatg attcgataaa gaagctaccg ctttccaccc
     1981 gggtggggta gatccggatg ccctgcgctt ccttcaggga gatgctgatg atcgtggagt
     2041 aattcgtttt cccatcgcgg tctaccatct cgaggcgata gtagagtttg ccgtcgtagg
     2101 tatcctggtc ggtgtaggaa tagctttgtt ctgtcgtatc gttggcgggg atggtatgaa
     2161 tgtcttcgaa ggaggacccg tcggtgctgc gctggaccac aaagtgatcg aagtcgacgg
     2221 cgttggtggc cgtccatttc aacagtgcct ggttgttgtc gcgtgcgctg ccggaccagc
     2281 tggtcagggt tacgggcagg gtggtagagg tttggacaaa gctgatgcga tcgtaaccgc
     2341 cattctggta atattccagc accatgttgg tagttccgct gagggctgcg ctataggtgg
     2401 aggtggtgta gctgtggtcg ttccagttat tgatcgccca ggtgttgccg ccgtcgaggc
     2461 tgaagcggta tccatcgtcg ccgccgacgg tgaagacgta tgacccggcg gccaggttct
     2521 gtgtgagccg gtagcgggcg gaaaactggt aggtctggac gctgcaactg ttggtattat
     2581 aggtgttcgt gttgctgccg ccggggttgt tgaagttctc gtcgaagttg gggccggagg
     2641 cgctgccttc ggtgacctgg cctttatacg taccgaaatt cataccctgg tagagatagc
     2701 cgatccaggt ggttccggtg ccgtagacgg tgggatctcc cgtgccggtg caattctgca
     2761 cgatggaaaa cgtgacgcgg ttggccccgc cgttctcgta gtattcgagc accaggttgg
     2821 tgctgccgct gagggtaacc gtatagctgg tggtggtata gccatggtcg ttccagttat
     2881 tgatcgccca cgtactgccg ccgtcgaggc tcagccgata gccgtcgtca ccgccgacgg
     2941 tgatggtata gttgccggag agggcctggg taagtttgta acgaacgcta aagccgtcgg
     3001 tatagaccga gcagccgttg gtgggatagt tcacctggtt gccgccaaag ctctcgtcga
     3061 agttggggct ggaggcagaa ccctcgttta catatccctg ataattattg aagttcatgc
     3121 cggtatagac atagccgatc cagacattat tggtaccgta cgtggtttga tttccctggg
     3181 cggtggcgca ggcttgagcg gagatccggc cgttggctgc cagaagaagg atcaggggca
     3241 gaatgaattt tttcgacagg gttttgtaaa c
//
LOCUS       DATGFG010001084         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_630759, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001084 DATGFG010000000
VERSION     DATGFG010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630759"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..718)
                     /locus_tag="VKQ52_20590"
     CDS             complement(194..718)
                     /locus_tag="VKQ52_20590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ89669.1"
                     /translation="MKDNSIQEIKKGDRNAFYTVFHEYHARLYQYIYNRTGSAWYAEE
                     TVQLSFIRLWEKREGLSEQYSLSIQLFRIAKSTLIDLTRKEKLRDTQELSDTFISGPT
                     ESERLLYKEQLEKVLAAMDELPARSKEVFQLSRLEDLSHKEISSQLSISPKTVEVHIT
                     KALKHLRKTFFFFF"
     gene            complement(886..2853)
                     /locus_tag="VKQ52_20595"
     CDS             complement(886..2853)
                     /locus_tag="VKQ52_20595"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HLZ89670.1"
                     /translation="MRSIAIATIGLILAGYSFFACTQPATPPEKLIAQKLLTQVDSFA
                     LICHAMEGALDSAGGASETTSRPPAAPTTAPGAGNKPQRPQRIQQLFLDARLAYKRFE
                     WAAEYFDPTAAHAVNGPPVPEAEMSGLVVPPTGLQVIETFLFPIVDTAKKAELRRQTR
                     ILLAACEKYKSHFANIDIFDWQVFDAAKLEVFRVMTLGITGFDDPLTLHSTAESAAAL
                     QSLQAVLAHYPDKTATTQGPAPQAATAKTPDNSSSASGTPDNSASGTPDNSVAGKISA
                     ATHYLSAHPDFNSFDRATFITNYANPLTTAISDQETALAIHVIRYNRLLNQDAKTLFD
                     TTAFNVNAYSPDPASFITPAKITLGERLFTDASLSSTGTRSCQSCHQPAKAFTDGLVK
                     NTVLGRNDPLPRNTPTLLNAALQPAQFYDMRVGTLEEQSLSVVQNNAEMHGSMKLSVQ
                     RLWQDTTYRRLFTTAFPRKDRTGIDTLEVMNAIGSYVRSLVALDSRFDRYMRGDHGAL
                     TTAEVNGFNLFMGKARCGTCHYMPIFNGAFPPRFVKTDAEVIGVPATQGAHAPVDADR
                     GRYAILPAPSFQHAFKTPSVRNAARTAPYMHNGVFTTLEQVIDFYDKGGGAGLGLHLD
                     NQTLPFDKLGLTPPEKSDLIAFIKSLDSRQR"
     gene            complement(2850..>3288)
                     /locus_tag="VKQ52_20600"
     CDS             complement(2850..>3288)
                     /locus_tag="VKQ52_20600"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLZ89671.1"
                     /translation="AKAGTLPAVSFVKPLGINNEHPGYSDVANGENHTVELINDVLNG
                     PNGKDALIIVTYDENGGFWDHVAPPVIDKKWGPGTRVPTILAGPFVKRGYVDHTQYET
                     VSILTLIEKRWKLDPLASRDRDALPLNNVFDFDLDQYGGGKYR"
BASE COUNT          574 a          732 c         1218 g          764 t
ORIGIN      
        1 ggggtcgggt tgaaaattct cccagctttg ttcgtccagg tcgtccgaat tgtacataca
       61 ctatattcgt ttggggagga aatatcccct agtcgcgggg aaaaaaattt gttcgggggg
      121 gtgcgctgga aaatagacag ggggcgtgcg ggaagggatg cgggtgacgc gctggaaagt
      181 atgcgggggg cgcctagaaa aagaagaaaa atgttttgcg cagatgtttc agcgcctttg
      241 tgatgtggac ctccactgtt ttgggggaga tggagagttg ggagctgatc tctttatggg
      301 aaagatcctc gagccggctc aattgaaaga cttccttgct gcgggcgggc agctcgtcca
      361 tcgcggccag cactttttcg agttgctcct tatataggag ccgttcgctt tcggtgggac
      421 ccgagataaa ggtgtcggat aattcctggg tgtcgcgtag tttttccttt cgggtcagat
      481 cgatcaacgt gcttttggcg atgcggaaga gttggatgga gagcgaatac tgttcggaga
      541 gtccctcgcg cttttcccat agccggataa agctgagttg gacggtttcc tcggcgtacc
      601 aggcgctgcc ggtgcggttg tagatatatt ggtaaagcct ggcgtgatac tcgtggaaga
      661 cggtatagaa ggcgttgcgg tcgcctttct ttatctcttg tatggagttg tccttcactg
      721 gttggctttg gctaattaca ggaacgcaaa ggtgtggtca aaaatacaaa acccggccga
      781 tgatggatta ttttaatatt aacactaatg cagccgattg cagtggcgcc tgatcgttgg
      841 cccaagacat aggggagtag tcggtcggct ccttttcatg ccgcctcagc gttggcgact
      901 gtcgaggctt ttgatgaagg cgatgagatc gctcttttcc ggtggggtga gaccgagttt
      961 gtcgaagggc agggtttggt tgtcgagatg taatccgagc ccggcgccgc cgcctttgtc
     1021 gtagaaatcg atcacctgtt cgagggtggt gaagacgccg ttgtgcatat agggcgcggt
     1081 tcgcgcggcg ttgcgtacgc tgggtgtttt aaaggcgtgt tggaaggagg gggcggggag
     1141 gatggcgtat ctgccccgat cggcatcgac tggtgcatgt gcgccttgtg tggcggggac
     1201 tccgatgact tccgcgtctg tttttacgaa gcggggcggg aaggcgccgt tgaagatcgg
     1261 catatagtgg caggtgccgc agcgggcctt gcccatgaag agattgaaac cgttgacctc
     1321 ggctgtggtg agggcgccgt ggtcaccccg catatacctg tcgaagcggc tgtcgagggc
     1381 gacgaggctg cggacatagg agccgatggc attcattact tcgagggtgt cgatgccggt
     1441 gcgatccttg cgcgggaagg ccgtggtgaa gaggcgacga taggtggtgt cctgccagag
     1501 gcgttggacg gaaagtttca tcgagccgtg catctccgcg ttgttttgta cgacgctgag
     1561 ggactgttct tcgagggtgc ccacgcgcat gtcgtagaac tgggcgggtt gcagcgcggc
     1621 gttcagtagt gtcggggtgt tgcggggcag aggatcgttc ctgcccagca cggtgttttt
     1681 gacgaggccg tcggtgaaag ccttggcggg ttggtggcag gactggcagc ttcgggtgcc
     1741 cgtggaggaa agggaggcat cggtgaagag acgttcgccg agggtgatct tggccggggt
     1801 gatgaaggag gccgggtcgg gtgagtaggc attgacgtta aaggcggtgg tgtcgaagag
     1861 cgtctttgcg tcctggttga ggaggcggtt gtagcggatg acgtggatgg cgagggccgt
     1921 ctcctggtcg ctgatggcgg tggtgagggg gttggcgtag ttagtgatga aggtggcgcg
     1981 gtcgaaggag ttgaagtcgg ggtgagcgga gaggtagtgg gtggcggcgg agatcttgcc
     2041 ggcgacggag ttgtccgggg ttccggaggc ggagttgtcc ggggttccgg aggcggacga
     2101 ggagttgtcc ggcgtctttg cggttgccgc ctgcggggcc gggccctggg tggtggccgt
     2161 tttgtcggga tagtgggcga gcacggcttg caggctttgg agggccgcgg cggattcggc
     2221 agtgctgtgg agggtgaggg ggtcgtcgaa gccggtgatc ccgagggtca tgacgcggaa
     2281 gacttccagc ttggcggcgt cgaagacctg ccagtcgaag atgtcgatat tggcgaaatg
     2341 cgatttgtat ttctcgcagg cggcgaggag gatccgggtc tggcggcgga gttcggcttt
     2401 ttttgccgtg tcgacgatgg ggaagaggaa tgtttcgatc acctgcaggc ccgtgggtgg
     2461 gacgacgagg ccggacatct ccgcttcggg gacgggcggg ccgttgacgg cgtgggcggc
     2521 ggtagggtca aagtattccg cggcccattc gaaacgctta tacgcgaggc gggcgtcgag
     2581 gaagagttgt tggattcgct ggggccgctg cggcttgttg cccgcgcctg gtgccgttgt
     2641 tggggcagcc ggcggcctgc tcgttgtctc ggacgcccct ccggcggaat ccagggcccc
     2701 ctccatggca tggcaaataa gcgcaaagct gtcgacctgg gtcaacagct tttgcgcaat
     2761 gagcttttcg ggtggcgtgg ccggttgtgt acaggcgaag aagctataac cggcgaggat
     2821 cagccctatc gtggcgatgg caatgctcct catctatatt ttccaccccc gtattgatcg
     2881 agatcgaaat cgaatacatt gttcagcggc agtgcatcgc gatcgcggga agccagcgga
     2941 tcgagtttcc agcgtttttc gatcagcgtc aggatgctga cggtctcgta ctgggtgtgg
     3001 tcgacatagc cgcgtttgac gaaggggccg gcgaggatcg tggggacgcg ggtgccgggg
     3061 ccccatttct tgtcgatgac gggcggggcg acgtgatccc agaaaccacc gttctcgtca
     3121 taggtgacga tgatgagggc atccttgcca ttgggtccgt taagaacatc gttgatgagt
     3181 tccacggtgt ggttctcgcc gttggccaca tcggaataac cggggtgttc gttgttgatc
     3241 ccgaggggtt ttacgaagga gacggcgggc agggtgccgg ccttggcc
//
LOCUS       DATGFG010001085         3852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_53444, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001085 DATGFG010000000
VERSION     DATGFG010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53444"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VKQ52_20605"
     CDS             complement(<1..629)
                     /locus_tag="VKQ52_20605"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HLZ89672.1"
                     /translation="MNTAPTFIPYDKLLGFTIDIFKTIGCPEKDAHTAAVALLSADLR
                     GIDSHGVARLIGYVRLQEAGRANTRPKVAIIHETPSTAVVDGDSGLGLVVAPFAMQVA
                     IEKAAVAGTGWVSVQNSNHFGIAGYHALQAVKKDMIGIAMTNASALVAPTFSLERLLG
                     TNPICVAVPAGEEPPFVADLATTTAANGKLEILQRKSEEAPLGWIQDSKG"
     gene            complement(802..1698)
                     /locus_tag="VKQ52_20610"
     CDS             complement(802..1698)
                     /locus_tag="VKQ52_20610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLZ89673.1"
                     /translation="MNKFLIGCLLCVSASFGLSGCGSSSGNGDTTKKDSTAAADSSSA
                     DVTKDWKIGVQMWTFHVAGPFVKALDKVDSAGVKYIEAFPGQPLGGDMKDTFGIRMSA
                     ASRAELKQLLQSKGIQIVAMGVIRPNTIAEWKQYFDLAKEFGLSYITAEPIKSQWDGV
                     DSLAGIYGINVAIHDHPRPNEYWHPDSVLAAVKGHSHIGSCADVGHWVRNGLDPVECL
                     KKLEGHVYGVHLKDVDSAGNTKANDRVVGTGVIHFTPIFQELKRQHFKGMFSIEREDN
                     WYNNVPDVITTIKFYNDQISKL"
     gene            1859..>3852
                     /locus_tag="VKQ52_20615"
     CDS             1859..>3852
                     /locus_tag="VKQ52_20615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89674.1"
                     /translation="MRRFLFFLCCFALLVGQVTAQNHTITGTVTDGNGAPIAGATVAV
                     KGTNRGTSTNGDGTFTVAVPANAKTLIISAVNFTAQEIDITGRTEVGTVALKPGNQSL
                     NEVVVVAYGTQKKTNLTGSVVMVNAAALADKPFTSVDKSLQGAVAGVQVSSVSGAPGS
                     STDIRIRGIGSINASSSPTWVIDGVIATTGDQSSNTTTANVLATLNPDDIESISVLKD
                     AASNAIYGSRGANGVILVTTKKGRAGKTRVALTAEVGQNSQAFNPDNKPMNSLQYRTI
                     LRQGLINAGYASDNTGADALITSSRGLGYPSNYTSINTNWRDVVQRNGNQGQYNLSVS
                     GGTDKTQVYFSMGAFNQIGTALASDFKRYNGTLSVSQKINDKITLSSTLMAGYSKQNT
                     PFNGGAFGNPILESFLLLPWYTPYNPNGSFRFGANDSLGEFPLAPSVFNPVVEAAYNF
                     NTYRQTSLRGNVQGEYKILNNLSFTSRYSGEYADISEDQYWNPFYGDGYATTVSAAGQ
                     GYSVYTRIYDWTWSNFFDFRQYLNADKDIYFDVKPGYEAFEYNKYNLQTGAKGFPQTL
                     DLKYLTSASTPTTSAVLPTSNSTSSEFVLGDFNYKDRYILSGSFRRDGSSVFGADHKY
                     GNFYSVGGSWNLNEENFMKGLPAFSLLKLRASYGATGNALG"
BASE COUNT          842 a         1028 c         1117 g          865 t
ORIGIN      
        1 cccttgctat cctggatcca gccgagaggc gcttcttcgc ttttgcgctg gaggatctcc
       61 agtttgccgt tggcggcggt ggtagtggcg agatcggcga caaagggggg ctcctcgccg
      121 gccgggaccg ccacgcagat cgggttggtg ccgaggaggc gttcgagcga gaaggtgggg
      181 gccacaaggg cgctggcgtt tgtcatggcg atgccgatca tatccttttt cacggcctgg
      241 agggcatggt aaccggcgat gccaaagtga ttcgagtttt gtacgctgac ccagccggtg
      301 ccggccaccg ccgctttttc gatggcgacc tgcatggcga agggggctac cacgagtccg
      361 aggccgctgt ccccgtccac caccgcggta gagggtgttt cgtggatgat ggcgaccttg
      421 gggcgggtgt tggccctgcc ggcctcctgg aggcggacat aaccgatgag ccgggcgacg
      481 ccatgagagt cgatcccgcg caggtcggcg ctgagcaggg cgacggcggc ggtgtgtgcg
      541 tccttttcgg gacagccgat cgttttgaag atatcgatgg tgaaccctaa aagcttgtca
      601 taggggatga aagtgggcgc tgtgttcatg cggtaaagat caccattttt atgaaaagac
      661 cttcatcacc ggcgggcaag gtattcctgg cggcgggggg gatgtcaggc aggtattcgt
      721 cgcggcaggc ttttccggca gcggatatgg gtatatttcc ggtggagagc agcaaagagg
      781 cccctcccgg ctgtctattg tttagagttt gctgatctgg tcgttataga attttatcgt
      841 cgtgataacg tccggaacat tgttatacca gttgtcttcc cgttcgatag agaacatacc
      901 cttgaaatgc tggcgtttca gctcctggaa aatgggagtg aagtggatga cgccggtgcc
      961 tactacccta tcgttagcct ttgtgttccc ggcggagtcg acgtctttga ggtggacgcc
     1021 atagacatgg ccttccagct ttttgagaca ttcgacggga tcgaggccgt tgcgtaccca
     1081 gtgccctaca tctgcgcatg agccaatatg agagtggccc ttcacggccg ccagaacgga
     1141 gtcggggtgc cagtattcat ttggacgggg atggtcatgg atggcgacgt tgatgccata
     1201 gatgccggcg aggctgtcca caccatccca ctggcttttt attggctcgg cggtaatgta
     1261 gctgaggccg aactcttttg ccagatcgaa gtattgcttc cattcagcga tggtattggg
     1321 ccggataaca cccatcgcta ctatctgtat ccccttggat tgcaggagct gtttcagctc
     1381 cgccctgctg gccgccgaca tccggatgcc gaaagtgtct ttcatgtcac caccgagggg
     1441 ctggccggga aatgcttcta tatatttcac tcccgcgctg tcgactttat caagcgcctt
     1501 tacgaatggg ccggcgacgt ggaaagtcca catctgtacg ccgatcttcc agtcctttgt
     1561 aacgtctgcg gaggaggagt cggcggcggc ggtggagtct ttttttgtcg tatcaccgtt
     1621 accggaagag ctaccgcagc cggatagccc aaaagaggcg gaaacacaga gcaggcaacc
     1681 gatcagaaat ttgttcatgt tacattgttt tgtatgttca gaaaaataaa gtactgaaat
     1741 taagcgtttt tacgacccaa tgtttgctaa actccggaca accctgtagc agttatatcc
     1801 gtttatcatt aaatttgtca atatatagtc aacatttatt cactacaaaa ctactcttat
     1861 gagaaggttt ctcttcttcc tatgctgttt tgccttgctt gtcgggcaag tgacagcaca
     1921 aaatcacacc attacaggga cggtaacgga tggcaacggg gctccgatag caggtgctac
     1981 cgtagcggtc aaggggacca atcgtgggac gagcaccaat ggtgacggga cgttcaccgt
     2041 cgcggtgccg gcgaatgcga agaccctgat catatcggcc gtgaatttca ctgcccagga
     2101 gatagacatc accggcagga ccgaggtggg gacggtagcc ttaaagccgg gcaatcagag
     2161 ccttaacgaa gtggtggtcg ttgcttacgg tacgcagaaa aagaccaacc tcaccggatc
     2221 ggtggtgatg gtgaatgcag cggccctggc ggacaaaccc tttacttcgg tggacaaatc
     2281 cctgcagggc gccgtcgccg gcgtacaagt aagctccgtt tccggcgcgc cgggttcttc
     2341 cactgatatc cgcatcaggg ggatcgggtc catcaacgca agttccagcc ccacgtgggt
     2401 catcgacggg gtgatcgcca ccacgggcga ccagtccagt aacacgacca cggcgaatgt
     2461 actggccacc cttaacccgg atgatatcga gagtatttcc gtgctgaagg atgccgcctc
     2521 taacgcgatc tatggttcga gaggtgcgaa cggggtcatc ctggtgacga cgaagaaggg
     2581 ccgggccggc aaaaccaggg tcgctctcac cgccgaggta ggccagaaca gccaggcctt
     2641 caacccggat aacaagccga tgaacagcct gcagtaccgg accatcctcc ggcaggggct
     2701 gatcaacgcc ggctatgcca gcgacaatac gggggccgat gcgctgatca ccagttcacg
     2761 cggactcggg tatccctcca actatacgag catcaatacc aattggcggg atgtggtcca
     2821 gcgcaacggc aaccagggtc aatacaacct gagcgtcagc ggcggtaccg ataagacaca
     2881 ggtctatttt tcgatgggcg cattcaacca gatcgggacg gcccttgcct cggatttcaa
     2941 acggtataat ggtacgcttt ccgtcagcca gaaaataaat gacaagatca cgttgagttc
     3001 tacgctgatg gccgggtata gcaagcagaa tacgccattc aacggcgggg cctttggaaa
     3061 cccgatactg gagtccttcc tcctgcttcc ctggtacacg ccttacaacc ccaacggcag
     3121 cttccgtttc ggagccaatg attccctcgg ggaattcccg ctggcgccga gtgtgttcaa
     3181 ccccgtagtg gaagcggctt acaacttcaa tacctaccgg cagaccagtc tgcggggaaa
     3241 tgtacagggc gaatacaaga tcctgaacaa tctttccttt acaagccggt attcggggga
     3301 atatgccgac atctccgagg atcaatactg gaaccccttc tatggcgacg ggtatgcgac
     3361 taccgtctcg gcggcgggcc agggttactc cgtttatacc cggatctatg actggacctg
     3421 gagcaacttt ttcgatttcc ggcaatacct gaatgcggac aaggatatct atttcgatgt
     3481 gaagccgggt tatgaagcct tcgagtataa taaatataat ctccagaccg gcgcgaaggg
     3541 atttccgcag acgctcgatc tgaaatacct gacatcagcg tccacgccga ccacctctgc
     3601 cgtgttgccg acatcgaatt ccacgtcttc ggagttcgtg ctgggcgatt tcaactacaa
     3661 agacaggtac atcctctccg gcagtttccg ccgggatgga tcttcggtct tcggggccga
     3721 ccataaatac ggtaattttt attccgtagg cggcagctgg aacctgaatg aagagaattt
     3781 catgaagggt cttcccgcct tcagcctgct gaaactccgc gccagctacg gcgcgacggg
     3841 taatgccctg gg
//
LOCUS       DATGFG010001086         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1317188, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001086 DATGFG010000000
VERSION     DATGFG010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317188"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /locus_tag="VKQ52_20620"
     CDS             <1..1884
                     /locus_tag="VKQ52_20620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89675.1"
                     /translation="LPVSPVKNSDGSYFEDFKTSNYYNPVAMMNHSQLNTKYNNLMAD
                     FTTQVKLPFGLTYDLNLAYLNLSTLVGEYLDSYFTSNYSGMYNNPDPGLSGHTQQTFG
                     TNGQATRQSYTTTNKILETYFTWNRKFGYHSINAVLGYSYQGNVIGNGFSATTYNFPV
                     NNIGYNNLAISNPYAYTTQINLGSDQYQDTRLISDFARVNYNFNDKYLIQGSIRRDGS
                     SVFGANNRWGWFPSVGAAWRITQEGFMRNQQTFTDLKLRGSYGVTGNSSGFSALTAQS
                     ISGLVGTYYYNGSTLAAYGPTQAQNVNLRWEKTSTTDIGVDFAILNNRVTGSIDWYNK
                     NTDGMIWSYGVDPILVPTGSIVANGGSMNNKGVELSLTAAVVKNSDFSWTSTLNLAHN
                     TNRITNLKNPLFSGGDSVRAGDPEGGGQSGSTLELLKTGHPLGQFFSLQYAGKNSSGI
                     SQYVSGSGQGYTTAPTIGTDYHYLGDAQPRLLAGWANTFKYRNWDLNIFLRGTFGNKI
                     FNATRADLFRPATAQYGNILVDAQNESIGDINSYKYSSRFIESGSYLRFDNATLGYNF
                     KNVGPYIKRLRAYASVNNLFVITGYKGVDPEVNQGGIAPGIDYNNFYPKTRTFLAGVN
                     VSF"
     gene            1905..>3172
                     /locus_tag="VKQ52_20625"
     CDS             1905..>3172
                     /locus_tag="VKQ52_20625"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ89676.1"
                     /translation="MKKIVQYILTPVLLLAAFSACHKVEVPVTTELTPDVYPSDSASF
                     ITASGPVYVVLRGNYGVEFYMAQTLSTDEAIMPARGGNWYDGAQNQQMHYHTYTKDNG
                     YVNGNWYWLSTIIGVANQTLSILQKTETAGASKNQNLAEIKMIRALAYFWMMDNYGNV
                     PIDTAYGDFTPHTNVARAKVFSFIESEVNAALPYLSSANNTSTYGRANKWTAYALLAK
                     MYLNAEYYTGTARYNDCITACDQIINSGLFSICPLASYLQMFYPTNGPTAAGSKNEFI
                     FAIPFDPNFNNGSWPFRGVNNQSRYDVPRSMGKVAAGGGYNFFNLTWTPGAPESTIPS
                     FYSYFYDANDVRNGQWLTGLQYQHDGVTPITSTTTYAGYDAITYAGNNNPYTYQLNLT
                     PNIILRQSTTSFDCGNDEVAWNMGYRDIKF"
BASE COUNT          772 a         1023 c          727 g          650 t
ORIGIN      
        1 cttcccgttt ctcccgtaaa gaacagcgac ggctcctatt tcgaggattt caaaacctcg
       61 aactattata accctgtcgc catgatgaac cacagccagc tgaacacgaa gtacaacaac
      121 ctcatggccg atttcaccac ccaggtgaaa ctgcccttcg gcttgacata cgacctcaac
      181 ctggcctatt tgaacctgtc cacgctcgtg ggtgagtacc tcgatagtta ttttacgagt
      241 aactacagcg gcatgtacaa caaccccgac ccgggtcttt caggccacac ccaacagacc
      301 ttcggcacca acggccaggc aacccgtcaa tcgtatacca ctaccaataa gatcctcgaa
      361 acctatttca cctggaaccg gaaattcggg tatcacagca tcaatgccgt actgggttat
      421 tcctaccagg gcaatgtcat cgggaatggg ttctctgcaa ctacgtacaa cttccccgtg
      481 aataacatcg gctacaacaa cctggcgata agcaaccctt atgcttatac aacgcagatc
      541 aacctcggtt ccgaccaata ccaggatacc cggctcatct ccgatttcgc ccgggtgaac
      601 tacaatttca acgataaata ccttatccag ggctccatcc ggcgcgacgg cagttcggta
      661 ttcggcgcca ataaccgttg gggctggttc ccctccgtcg gcgcagcctg gcgcatcacc
      721 caggaaggct ttatgcgcaa ccagcagacc tttaccgacc tgaagctccg cggcagctat
      781 ggcgtcaccg gcaattccag cggcttcagc gccctcacgg cccagtccat ctccggcctt
      841 gtaggtacct actactacaa cggctcgacc ctcgcggcct atggacctac ccaggcgcag
      901 aatgtcaacc tccgctggga aaagacctcc accaccgaca tcggcgtcga ctttgccatc
      961 ctcaacaacc gggtcaccgg ctccatcgac tggtacaata agaacaccga tggcatgatc
     1021 tggagctacg gggtcgaccc gatcctggta cccaccggct ccatcgttgc caacggcggc
     1081 agcatgaaca acaagggcgt cgagctgagc cttaccgcag ccgtggtaaa gaatagcgac
     1141 ttctcctgga ccagcaccct caacctcgcg cacaacacca acaggatcac caacctcaag
     1201 aaccccctct tctccggcgg cgactcggta cgcgcaggcg atccggaagg tggagggcag
     1261 tccggcagca ccctcgaatt gctgaagacg ggtcacccgc tgggccagtt cttctcgctg
     1321 caatatgcag gcaagaattc cagcggcatc tcccagtatg tttccggttc cggccagggc
     1381 tacaccactg cgcccaccat cggcaccgat taccactacc tgggcgatgc acagccccgg
     1441 ctcctggcag gttgggccaa caccttcaag tatcgcaact gggatctgaa catcttcctg
     1501 cgcggaacat tcggtaacaa gatcttcaat gctacccgcg ccgacctgtt caggcccgca
     1561 accgcccaat acggcaatat cctcgtggat gcacagaatg aatcgattgg cgatatcaat
     1621 tcgtacaaat actcttcccg tttcatcgag agcggcagct acctgcggtt cgacaatgcc
     1681 accctgggct acaatttcaa aaatgtcggc ccctatatca aacggctcag ggcctacgcc
     1741 tcggtgaaca acctcttcgt gatcaccggc tataagggag tcgaccccga ggtcaaccag
     1801 ggcggcatcg ctccgggtat cgattacaac aacttctatc cgaagacccg cacgttcctg
     1861 gcaggtgtaa acgtatcctt ctaaacattt aaaaaaagag tatcatgaaa aagatcgttc
     1921 aatatatatt aacaccggtg cttctcctgg cggcgttctc ggcctgccat aaggtggagg
     1981 tgcctgtaac taccgaactc acacccgatg tctatccttc ggactcggct tcctttatca
     2041 cagcttccgg tcccgtctat gtcgttctac gcggcaacta tggggtggag ttctacatgg
     2101 cgcaaaccct cagcaccgac gaggccatca tgcccgccag gggcggcaac tggtacgacg
     2161 gcgcccagaa ccagcagatg cattatcaca cctacaccaa ggacaacggc tatgtcaacg
     2221 gcaactggta ctggctttcc accatcatcg gggtcgccaa ccagaccctt tccatcctgc
     2281 aaaagaccga aacggccggt gcgtcgaaaa atcaaaacct ggccgaaata aaaatgatcc
     2341 gcgccctggc ttatttctgg atgatggaca attatggcaa cgtacccatc gacaccgctt
     2401 acggggattt tactccacac accaatgtgg cgcgggccaa ggtgttcagt ttcatcgaaa
     2461 gcgaggtcaa tgccgcactg ccgtatctga gcagcgccaa caatacctcc acctatggcc
     2521 gggccaataa atggacagcc tatgccctgc tggccaagat gtacctcaat gcggaatact
     2581 acaccggcac ggcccgttat aatgactgta tcaccgcctg tgaccagatc atcaattcag
     2641 gtctgttctc gatttgcccg ctggccagct atctccagat gttctaccct accaatggtc
     2701 ccacggctgc gggcagcaag aacgaattta tcttcgccat ccccttcgat cccaatttca
     2761 acaatggctc ctggcccttc cgtggtgtga ataaccaatc gcgttacgat gtaccccgca
     2821 gcatgggcaa ggtggccgcc ggcggtgggt acaatttctt caatctcacc tggacgcccg
     2881 gcgcgcccga gagcaccatc ccttccttct attcctattt ctatgacgcg aacgatgtcc
     2941 gcaacgggca atggctcacc ggtttgcaat accagcatga cggcgtcacc ccgatcacca
     3001 gcacgaccac ctatgcaggg tatgatgcca tcacctatgc cggcaacaac aacccgtaca
     3061 cctatcagct caacctgacg ccgaatatca tcctgcgcca gagtaccact tccttcgact
     3121 gcggcaacga cgaagtggcc tggaacatgg ggtaccggga tatcaaattc ta
//
LOCUS       DATGFG010001087         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_748459, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001087 DATGFG010000000
VERSION     DATGFG010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748459"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1197
                     /locus_tag="VKQ52_20630"
     CDS             37..1197
                     /locus_tag="VKQ52_20630"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLZ89677.1"
                     /translation="MKLFDVYPINDITIVKAKGSYVWDQEGQQYLDLYGGHAVISIGH
                     THPHYVQRITDQLHKVGFYSNSIHIPLQEELANKLGKVSGRPEYQLFLCNSGAEANEN
                     ALKLASFHTGRKKVVAFRKSFHGRTSLAVAVTDNPAIVAPVNQTENVLFLPFNDEAAL
                     EACFAEQGKEIAAVIIEGIQGVGGINEAAASFLQAISRLCKKYGAVFIADSVQCGYGR
                     SGKFFSQDHAGVDADIYSMAKGMGNGFPVAGIIIAPHLQARHGMLGTTFGGNHLACAA
                     AVAVLEVIEKENLIANAAEVGNYLMAELKKIPQLQRVRGKGLMIGFDVPEALKDLRKN
                     LLWRQHIFTGEAKPNTIRLLPSLALRRKDADAFLEAIQEEIHAMEGEGVKVN"
     gene            1205..2164
                     /locus_tag="VKQ52_20635"
     CDS             1205..2164
                     /locus_tag="VKQ52_20635"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89678.1"
                     /translation="MRNFVSAADVTDIDSLVKAALAYKADPWKDKTLGANKRVGMLFL
                     NPSMRTRLSTQIAAQNLGAEAIIFNVDKDGWALEFEEEAIMSGNTVEHVKDAAPIMGH
                     YFDILCIRTFPSLKNKVDDYSELYINQFIKYAGIPVVSLESATLHPLQSLTDIITITE
                     VLGKTARKPRIVLSWAPHVKALPQCVGNSFAQWVNAWGKADFVITHPEDYELDTRFTK
                     GATITHNQDEALKDADFVYVKNWSTYTDYGKIYNNDPEWMLTNEKMKATNNARVMHCL
                     PVRRNVELSDEILDGPSSIVTQEASNRVWAAQAVLAEILRSKK"
     gene            2168..>2416
                     /locus_tag="VKQ52_20640"
     CDS             2168..>2416
                     /locus_tag="VKQ52_20640"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89679.1"
                     /translation="MQLSIIKIGGNIIDDEAKLSAFLDVFAAVPGKKILVHGGGKLAT
                     KVAEGLGIRQQLVDGRRITDAETLRVVTMVYAGTINKGI"
BASE COUNT          562 a          536 c          782 g          536 t
ORIGIN      
        1 tgcggttgaa agcaaatgca ttttaaaaag aagtatatga aattattcga cgtttatcct
       61 atcaatgaca tcaccatcgt aaaggcaaag gggtcttatg tttgggacca ggaagggcag
      121 cagtatctcg acctttatgg cggccatgcc gtgatctcta tcgggcatac gcatccgcat
      181 tatgtacagc gcattacgga tcagctccat aaagtgggtt tttattccaa ttcgatacat
      241 atccccctgc aggaggagct ggcgaacaag ctggggaagg tgtccggccg gccggaatac
      301 cagttgttcc tttgcaattc cggggcggag gccaatgaga atgccctgaa gctggcgagt
      361 tttcataccg gcagaaagaa ggtggtggct ttccgcaagt cctttcatgg ccggaccagc
      421 ctggccgttg ccgttacgga taacccggcg atcgtagccc cggtgaatca gacggagaat
      481 gtcctcttcc tgccttttaa tgacgaggcg gcgctggagg cgtgttttgc ggagcagggt
      541 aaggagatag cggcggtgat catcgaaggc atccagggtg tcggcgggat caatgaggcg
      601 gctgcgtcct ttctacaggc gatcagccgg ctttgcaaga aatatggcgc ggtgtttatc
      661 gccgacagtg ttcagtgtgg ttatgggcgt agcgggaagt tcttttcgca ggatcatgcc
      721 ggggtggatg cggatatcta tagcatggcc aagggcatgg ggaatggttt cccggtggcg
      781 gggatcatca ttgcgccgca tctgcaggcc aggcatggca tgctgggcac tacgtttggc
      841 ggcaatcatc ttgcctgtgc cgcggccgtg gccgtgctgg aagtcatcga aaaggagaat
      901 cttatcgcca atgccgcgga ggtagggaat tacctgatgg cagagctgaa aaagataccg
      961 cagttgcagc gggtgcgtgg gaaggggctg atgatcggct tcgatgtgcc ggaggcgtta
     1021 aaggatctgc ggaagaacct gctctggcgt cagcacatct tcacggggga agccaagccg
     1081 aatacgatcc ggctgctgcc ttcgcttgct ttgcgcagga aggatgcgga tgcctttttg
     1141 gaggccatac aggaggagat ccatgcaatg gaaggcgaag gggttaaagt aaactagcat
     1201 aaaaatgaga aattttgttt ccgccgccga tgtcaccgac atcgacagcc tggtaaaggc
     1261 ggccctggcc tataaggccg atccgtggaa ggacaagacg cttggcgcga ataagcgtgt
     1321 aggcatgctt tttctgaatc ccagtatgcg tacccggctg tcgacgcaga tcgcggccca
     1381 gaacctcggg gcggaggcga tcattttcaa cgtcgacaag gatggctggg cgctggagtt
     1441 cgaggaggaa gcgatcatga gtggaaatac ggtggagcat gtaaaggatg cggcgccgat
     1501 catgggtcat tatttcgaca tcctttgtat ccggaccttt ccgtcgctga agaataaggt
     1561 ggatgattat agcgagctgt atataaatca gttcatcaaa tatgcgggca tcccggtggt
     1621 gagtctcgag agcgcgacgc tgcatccgct gcagagtctt accgatatca tcaccatcac
     1681 ggaggtattg gggaagacgg cgagaaaacc caggatcgtg cttagctggg cgccgcatgt
     1741 aaaggcgttg ccgcagtgtg tggggaacag ttttgcgcag tgggtgaatg cctggggcaa
     1801 ggcggacttt gtcattacac atccggaaga ttatgagttg gacacgcggt tcacgaaggg
     1861 cgccaccatc acgcataacc aggatgaggc gttgaaggat gcggattttg tgtatgtcaa
     1921 gaactggagt acgtatacgg attatgggaa gatctacaat aacgacccgg agtggatgct
     1981 caccaatgag aagatgaagg cgacgaataa tgccagggtg atgcattgtc tgccggtgcg
     2041 gcggaatgtg gagctcagcg atgagatcct ggacggtcct tcgagcatcg tcacgcagga
     2101 ggcttcgaac cgggtgtggg cggcgcaggc cgtgttggcg gagatcctgc gcagcaaaaa
     2161 atagtgtatg caactttcca ttatcaagat aggcgggaat atcatcgatg acgaggcaaa
     2221 gctttctgct tttctcgatg tatttgccgc tgttccgggg aagaagatcc tggtacatgg
     2281 gggtggtaaa ttggccacga aggtggcgga ggggcttggc atccggcagc aattggtgga
     2341 tggcaggcgg atcaccgatg ccgagacctt gcgggtggtg acgatggtct atgcgggtac
     2401 gatcaacaag ggtatc
//
LOCUS       DATGFG010001088         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1570948, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001088 DATGFG010000000
VERSION     DATGFG010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570948"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VKQ52_20645"
     CDS             complement(<1..1646)
                     /locus_tag="VKQ52_20645"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLZ89680.1"
                     /translation="MNIKKTGLLPVLILVALTSARAQGSVRYLGIEDGLSNNAVTQIY
                     QDNRGFMWFGTYDGLNRYDGYKFKVFRNRIGDSSSLVDNNINRIAGASDCRLWVGGRK
                     GLSVFNPVTETFIMPRYETDYDKTAHPLRDNILSIKAIAGDRVLVGTEHLGLFEFDAR
                     SGVGKQIPLIGSPSYEANAVAADPVGRRYWIFVQHEGLCRFDTAAHRLQLVSVFTPKT
                     NSITADRQGNIWLGTDNGLFKYDVTAGRISGNYLPAASKVMNLYLDRRGVLWIACDGS
                     GILLLDPGESKAQPLTFHDRRQGLSSNSVYAIYEDKENRKWIGTLRGGVNIIDPSKNP
                     FELHTFPTDNAFGLTNNFIFSFSEAADGNIWVGTDGAGLRYWNRRTNASTVFTANAGA
                     RGSISSNFITSILQDSRQDTWVGTWFGGINRYIPSDRSFRHYTCFNPLTRSEENKVWI
                     VYEDKDRRLWASTSNNGTLYLYDPAGDHFEAVDPAGISNLQCLAEDKRGGFWGGTYTA
                     LVKIDRVNKKHLVYPIGYPVRCIREDAHGNFWVGTDGGGL"
     gene            1778..>2114
                     /locus_tag="VKQ52_20650"
     CDS             1778..>2114
                     /locus_tag="VKQ52_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89681.1"
                     /translation="MVARLPIKSLVRLNWLLSLVSSAIVFLFLIQNEPERDAFLYTAM
                     TAVIMGIIGYANIGILVYLFRRYTFRSKVFKITRYAATYLVSEAAYFGIWPLFSQFSA
                     GGHSYRERGV"
BASE COUNT          452 a          620 c          533 g          509 t
ORIGIN      
        1 aaaagcccac cgccgtcggt gccgacccag aaattcccgt gcgcatcttc ccggatacag
       61 cgaaccgggt agccaatggg gtacaccaga tgttttttat tgacccggtc tatctttacc
      121 agcgcggtat aggtgccgcc ccaaaaacct ccccgcttat cttccgccag acactgcaga
      181 ttgctgatcc ccgcagggtc caccgcttca aaatggtcgc cggcaggatc atagagatac
      241 agcgtaccat tattactggt gctggcccat aaccgacggt ccttgtcctc atacacgatc
      301 cacaccttgt tctcctccga tcgtgtaagt ggattaaagc aggtatagtg ccggaacgat
      361 ctgtccgatg ggatataccg gttgataccg ccaaaccatg ttcctaccca ggtatcctgc
      421 cggctatcct gcaggatgga ggtgatgaaa ttactgctga tcgatcccct ggcgccggca
      481 ttcgccgtaa agacggtgga agcattcgtc ctgcggttcc aataccgcag acctgcgcca
      541 tcggtgccca cccatatatt cccgtcggcc gcttcactga acgagaaaat aaaattattg
      601 gtcaggccaa aggcattatc cgttggaaag gtatgcagct cgaacgggtt cttcgaaggg
      661 tcgatgatat tgacgccacc gcgaagggtg ccgatccatt tccggttttc tttatcctca
      721 tagatggcat acaccgaatt gctgctcaat ccctgtcgac ggtcatgaaa ggtgagtggc
      781 tgtgccttgc tttcgcccgg atccaataaa agaatgccgc ttccatcgca ggcgatccaa
      841 aggacacccc gtctgtccag atagaggttc attaccttac ttgccgcggg caggtagtta
      901 cccgagatcc tccctgccgt cacatcgtat ttaaaaaggc cgttgtcggt tcccagccag
      961 atattcccct ggcgatctgc cgtgatgctg ttcgtcttcg gggtgaacac ggaaaccagc
     1021 tgtagccggt gagcggcggt atcgaagcgg caaagccctt catgctgtac gaagatccaa
     1081 tatcttctac cgacgggatc ggcggcaacg gcattcgctt cataagaagg agaaccgatg
     1141 agcgggatct gttttcccac accgctcctc gcgtcgaact caaaaagccc caggtgttcg
     1201 gtaccgacca gcacccggtc tcccgcgatg gccttgatgg aaaggatatt gtcccggagt
     1261 ggatgggccg tcttgtcgta atccgtctca tatcggggca tgatgaacgt ctccgtcacc
     1321 gggttgaaaa cgctgaggcc cttccggcca cccacccaca aacgacagtc cgaggcgccc
     1381 gcgatccggt tgatattatt gtccaccagg gagctgctgt ccccgatcct gttcctgaat
     1441 accttgaatt tatacccgtc ataccgattg agcccgtcat aggtgccaaa ccacatgaat
     1501 ccccggttgt cctggtagat ctgtgtcacc gcgttattcg acaggccgtc ctcgatgccc
     1561 aggtagcgca ccgatccctg tgcacgggcg ctggtcaggg cgacgaggat caacacggga
     1621 agaagacccg tttttttaat gttcatatga cacttatcag ccatcggccg ataaaaataa
     1681 ggtaattgtc cgtacttatc cgcttcaccc ccgattttgt ccgcttcgag cggttcccac
     1741 ccccgttact ggacataaat cgcctacttt tgtttgtatg gtcgcccgtt tgcccatcaa
     1801 aagcctggtt cggttgaact ggttgttgtc cctggtctcc agcgccatcg tattcttatt
     1861 ccttattcag aatgagccgg aaagggatgc attcctgtac acggcgatga ccgccgtgat
     1921 catgggcatc atcggctacg ccaacatcgg catattggtg tatctcttcc gtcgctatac
     1981 gttcaggtcg aaggtcttta agatcacccg ctacgccgcc acctacctgg tcagtgaggc
     2041 cgcctatttc ggtatatggc cgcttttttc gcaattttcc gccggcgggc actcttaccg
     2101 ggaacggggc gtgg
//
LOCUS       DATGFG010001089         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_863614, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001089 DATGFG010000000
VERSION     DATGFG010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863614"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1506
                     /locus_tag="VKQ52_20655"
     CDS             265..1506
                     /locus_tag="VKQ52_20655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HLZ89682.1"
                     /translation="MDNTLMIPRKTKSSFWQDIVLRLMTRMHQGRLDLELPNGEQLVI
                     GNGIEPIRACIRIHDNAFFKHCILYGDIGLGEAYVEGLWDTDDIAAVISWVLLNLDKA
                     SGIPGNRTPALLVNLLNWWNRWKHIRRVDTPAGSRRNNAAHDHPNNDFFRLFLDPTMT
                     YSAAYFRRDGMTLEQAQFAKYERLCSQLHLRATDHVLEIGSGWGGSAIYMARQYGCRI
                     TTLTISAEQYQLSTQRIKAAGLESRITVLLRDYRNFSGRFDKIVSVEMSEAVGHRYLD
                     TYFKKCNEWLKRDGILAFQVITCPDSRYDSLRKSVDWIQKYISPGPLPSVAAINAAVN
                     RSGEMTLVDLKDLGLHYAATLKVWFMQFNSRLTEVKALGFDNHFIRKWNYYLCYCEAA
                     FRMRNIHVMQLVYTRPNNLSR"
     gene            complement(1530..>2576)
                     /locus_tag="VKQ52_20660"
     CDS             complement(1530..>2576)
                     /locus_tag="VKQ52_20660"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLZ89683.1"
                     /translation="AILPAALFCALTFHPLVKAESMRNAWWLAGTMILFFVSATTYII
                     PYNALLPELAETPGEKVRLSSYQQVGFVLGIIVSALVNNIADAVQIGFGITDRDIALQ
                     ITIAGLCVLAAVILAIPVLVLDGKRYSDSKPSHLSLWPAVRQTFASPNFRYYLISDFS
                     YYMALSIISSGLLYFVTVLLRLPESESGILTATMVLVSLVFYPLINFLAARWGKKPVV
                     LVSFAALSLIFIMIYFLGRLPASPKTQIFTLVICAAFPLASLGILPNAILAEIAQQDA
                     VRTGENREGMFFAVKYLFVKLGQTLGIALFAFLTVYGKDPGHDQGLRLNGVCGMVLCL
                     LAFIFFSRFRETGR"
BASE COUNT          653 a          861 c          608 g          454 t
ORIGIN      
        1 gatgggcgaa ctcttccaga acaaccatca caaaagcccc aacgatgcca acttcgcccg
       61 gaaatggttc gagctcgatc ccacctggcc cgttatccgg ctgctgcacg cactccggat
      121 catccggttg agaaaggtat aaccctgcga taggaagcag gccattcccg aaattatttg
      181 gccaatcgct tcaacaaggg aaccagacca tgttaatcct cgtatatcca acagacaggc
      241 gccgtcatca taagagtaac agtcatggat aatacgctca tgattccccg aaaaacaaaa
      301 agcagcttct ggcaggatat cgtcctgcgc ctgatgaccc gcatgcacca gggccggctc
      361 gacctggaac tgcccaatgg cgaacagctc gtcatcggca acggcatcga acccatccgc
      421 gcctgcatcc gcatccacga caatgccttc ttcaaacatt gtatccttta cggtgacatc
      481 ggcctggggg aagcctatgt ggaaggcctc tgggataccg acgacatcgc cgccgtcatc
      541 tcctgggtgc tgctgaacct cgacaaggct tccggtatcc cgggtaacag gacccccgcc
      601 ctcctggtca acctcctgaa ctggtggaac cggtggaaac atatccggcg cgtcgatacg
      661 ccggccggct cccgccgcaa caacgcagcc cacgaccatc cgaacaacga cttcttccgc
      721 ctcttcctcg atccgaccat gacctattcc gccgcttact tccgccgcga cggcatgacg
      781 ctcgaacaag cgcagtttgc caaatacgaa cgcctctgtt ctcaactgca tctccgcgcc
      841 accgaccacg tgctcgagat cggcagcggc tggggcggca gcgccatcta tatggcccgg
      901 caatatggct gccgcatcac caccctcacc atctccgcgg agcaatacca gctatcgacc
      961 caacggataa aagcggccgg cctcgaatcc cggatcaccg tcctcctccg ggactaccgc
     1021 aacttttcgg gccgcttcga caagatcgtc tccgtcgaaa tgtccgaagc cgtcggtcac
     1081 cgctatctcg acacctattt caaaaaatgc aacgaatggc tcaaacgcga cggcatcctc
     1141 gccttccagg tcatcacctg ccctgactcc cgctatgata gcctgcgaaa aagcgtcgac
     1201 tggatacaaa aatatatctc cccgggcccc ctgccctccg tcgccgccat caacgcagcc
     1261 gtgaaccgat cgggtgagat gaccctcgtc gacctcaaag acctcggcct ccactacgcc
     1321 gccacgttaa aagtctggtt catgcaattc aacagccggc tcaccgaagt aaaagccctc
     1381 ggcttcgaca atcacttcat ccgtaaatgg aattattatc tctgctactg tgaggccgcc
     1441 ttccgcatgc gcaatatcca cgtcatgcag ctcgtctata caagaccgaa caacctgtcc
     1501 cggtgacgga ggacgcccca ccccgggcct caacgccccg tctcccgaaa cctgctgaag
     1561 aagataaacg ccagcaggca caacaccatc ccgcagacgc cgttcagccg caacccctgg
     1621 tcgtgaccgg ggtcctttcc atataccgtc aggaacgcaa acaaagcgat ccccagcgtc
     1681 tgacccaact tcacaaaaag atacttcacc gcaaagaaca tcccctcacg gttctccccc
     1741 gtccgcaccg catcctgctg cgcaatctcc gcgagaatgg cattcggcag gatccccagc
     1801 gacgccagcg gaaatgccgc acagatcacc agggtgaata tctgcgtctt cggcgatgcg
     1861 ggaagcctgc caagaaaata gatcatgata aagatcaggc tcagcgccgc aaacgaaacc
     1921 agcaccaccg gcttctttcc ccaccgcgcg gcaaggaaat tgatcagcgg atagaaaaca
     1981 agcgatacca gcaccatcgt cgccgtcagg atcccactct cggattcggg tagccgcaac
     2041 aatacggtga caaaatacaa caatccgctg gagatgatac tcaacgccat ataataggag
     2101 aaatccgaga tcaggtaata ccggaagttg ggactggcaa aggtctgcct gaccgcgggc
     2161 cacaacgaca gatgtgacgg cttgctgtcc gaatagcgtt tcccatccag cacgaggacg
     2221 ggtatcgcca ggatcaccgc cgcgagaaca caaagccccg caatggtgat ctgtaacgca
     2281 atatcccggt cggtgatacc aaacccgatt tgcaccgcat ccgcaatatt attcaccagc
     2341 gccgagacga tgatccccag cacaaatccc acctgctgat aggaggaaag ccgcaccttc
     2401 tccccgggcg tctccgccag ttcgggcaac aaagcattat aaggaatgat ataggtggtc
     2461 gcggaaacaa agaacaggat catcgtcccg gccagccacc aggcattgcg catactctcc
     2521 gccttcacaa ggggatgaaa ggtcagcgcg cagaaaagcg cggccggcag aatggc
//
LOCUS       DATGFG010001090         6609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1662947, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001090 DATGFG010000000
VERSION     DATGFG010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662947"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VKQ52_20665"
     CDS             <1..1303
                     /locus_tag="VKQ52_20665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HLZ89684.1"
                     /translation="LLSLSPAVLPLAQNSLLTWAYSRFHRSRELSPVHYFSDEEAARL
                     APTSHQLSSTVRDTTKKNVVLFILESFSRCYLVPGDPRKANTPFLDSLLRKSLFFPHA
                     FSNGFTSNQGIVSILGGLPALMDEPFFYSEYANTPLQSVGNILRGKGYNTNFLMGAGK
                     DHFGFGTFAHMAGLDHTYWQNDFNDDRFYDGNWGIYDEPFLQYGARVLSEKKEPFLGV
                     FFTISAHPPYAIPPDLRSRFALPGKTPAQQSIGYVDYALREFFNTCRDKPWFRHTIFV
                     FSADHWLDTEDGKTPFSCLNSCTIPIFVYDPSADTADWRPTVAGQVDIAPTILDKLGY
                     RGSYTGFGRSLLDTTVADSDRYVINRIGETCQIITDKYILGFDILRNKSSYLYHYPTD
                     SACRKDLLKEGDGGPARRKLETLIRANIQSYRAALTKRKLD"
     gene            complement(1329..1574)
                     /locus_tag="VKQ52_20670"
     CDS             complement(1329..1574)
                     /locus_tag="VKQ52_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89685.1"
                     /translation="MFLPAGKPVFPLFPVKPALPSALPPFRISAVGGPLGAVGGDYYV
                     NHLGFFCKQELAIQKTLRLPVSFRLGTLEYCNKLEGK"
     gene            1787..3265
                     /gene="rpoN"
                     /locus_tag="VKQ52_20675"
     CDS             1787..3265
                     /gene="rpoN"
                     /locus_tag="VKQ52_20675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HLZ89686.1"
                     /translation="MSLSQSLQQKLLQKLSPQQIQLMKLLQVPTANLEERIKEELEEN
                     PALEVTEDHEDGEETKDEFDSAEEFEEADGSEDEYDNIDISEYVNDSDDEIADYKLRD
                     DNYPEADEDRVMPHRVETSFHELLLDQLGMLVVDDRTRKIAEQIVGSIDDDGYLRRET
                     ASIADDLAFRQNIETSEEEIESLIKKIQQFDPPGVAARDLQECLLNQLRRKKNGNRSV
                     DTAIDVLTYYFDEFTKKHYDKIQRGLNLSDDQLKEVINQIIKLNPKPGGDVTEVNKAE
                     SYVIPDFFITNSAGKLELSLNSKNAPDLRISEGYKEMLKEYDRGSKKDKRQKEAVLFI
                     KQKIDAAKWFIDAIKQRQQTLYNTMHTIMDYQHDFFLTGDETTLRPMILKDIAERTAL
                     DISTVSRVANSKFVQTEFGTYRLKFFFSESLSTDSGEEVSTREVKKILSDLIEGESKK
                     KPLSDEKLTELLQEKGYNIARRTVAKYREQLNIPVARLRKEL"
     gene            3262..3894
                     /locus_tag="VKQ52_20680"
     CDS             3262..3894
                     /locus_tag="VKQ52_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89687.1"
                     /translation="MIKKALAQFFSIVFHPLFISAYVMAFIIFVHPYAFAGFEHRQKV
                     MRLLTVVLCNTALPLFAVFLLWRLQLIKSPMLRNEKERIGPYLIAMIFYFWTAYVFKR
                     LPDTPPLAVHFLGGAFLAVCAAWVCNIYFKISMHAIAMGGATMFFCLFGLTDEFGSGL
                     YIAAALLLTGLVCTSRLLLSAHSSFEVWAGIFIGLFTQFAAWQWPWLLFF"
     gene            complement(3987..4709)
                     /locus_tag="VKQ52_20685"
     CDS             complement(3987..4709)
                     /locus_tag="VKQ52_20685"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HLZ89688.1"
                     /translation="MRRSLFHPGFWCVLVAFVLPVSVLRAGGPVKTKSSARAAAFAAE
                     VTNLYEEFDLKEVGLTKKAFEYALKGYYYLLDHHWLNKTNILSICDLSQSSRSKRLYV
                     LDLDQRKVLVNTYVAHGRNSGQEFARSFSNNPSSHKSSLGFYVTQGTYFGNNGLSLKI
                     RGMERGFNDRAGGRNIVVHGSQYVGPDFLEMNQFCGRSYGCPAVPADESETIIDLIKE
                     GSCLFIYHPTKKYTARSKILNS"
     gene            complement(4907..6313)
                     /locus_tag="VKQ52_20690"
     CDS             complement(4907..6313)
                     /locus_tag="VKQ52_20690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLZ89689.1"
                     /translation="MSTSQPNQKGNTFSTIALVLRQSLSGQHLDYTKGSIRRAVILLS
                     IPMILEMCMESVFAIVDIFFVGRLGQDAVATVGLTESVLTLVYSVAIGLSMAATAMVA
                     RRIGEKNPEEASHTAAQSIVISLLLTVVISVAGAFLAPHILGIMGASAATVAIGSAYT
                     RLVFAGSLVIMLLYLINGIFRGAGNAAMAMWSLWIANGCNIILCPILIHFYGLAGAAM
                     ATIIGRGVGVCYQLFHLLRGSGIIRLVRKQFLPDGAIIRGLSKIAWTGATQFLIGSAS
                     WIVMARIIASFNDTAAIAGYQVSIRILLFFLLPAWGMSNAAATLVGQNLGAGSPERAE
                     QSVRTASIYNAAFMLFVSLLFLFVAGPIVSFINRDPAVEEVAIRALRIISIGYVFYGV
                     GMVLTNAFNGAGDTRTPTIIGLFCFWAFQIPLAWALAIALGLGPKGVFLAILITETSI
                     TIVSFVVFRRGRWKRVKV"
BASE COUNT         1691 a         1922 c         1653 g         1343 t
ORIGIN      
        1 cctgctctcc ctctctccgg cagtcttacc cctcgcgcaa aacagcctgc tcacctgggc
       61 ctatagccgc tttcatcgca gccgcgaatt gtcacccgta cattatttct ccgacgaaga
      121 agccgcccgg ctggcgccca ccagccatca attgtcttcg acggtgcgcg acacgacgaa
      181 aaagaacgtc gtcctcttta tcctcgaaag cttctcgcgc tgttacctgg tcccgggaga
      241 cccccgtaag gccaacactc cttttctcga ctcgctgttg cgcaagagcc tgttctttcc
      301 ccacgccttt tccaacgggt ttacctccaa ccaggggatc gtctccattc tcggcggtct
      361 cccggcactg atggacgaac ccttcttcta ttcggaatat gccaatactc ccttgcaaag
      421 cgtcggaaat atcctccgcg ggaaaggcta taacaccaat tttctcatgg gcgcggggaa
      481 ggaccatttt gggttcggaa ccttcgccca tatggcgggc ctggatcaca cgtattggca
      541 aaacgacttc aacgacgatc gcttctatga cggcaactgg ggcatctatg acgaaccctt
      601 cctgcaatat ggcgcccggg tgctgtcgga aaaaaaagaa cccttcctcg gcgtcttctt
      661 taccatttcg gcgcatcccc cctatgcgat accgccggac ctccggtccc gctttgccct
      721 gcccgggaag acccccgcgc aacaaagcat cgggtatgtg gactatgcgc ttcgggagtt
      781 cttcaacacc tgccgggata aaccctggtt tcgccatacg atctttgtct tcagcgccga
      841 tcactggctg gataccgaag acggcaaaac ccccttctcc tgtctcaata gctgcaccat
      901 ccccattttt gtctatgacc cctccgcgga caccgccgac tggcgcccaa ccgtcgccgg
      961 ccaggtcgat atcgccccga ccatcctgga caaattgggc tatcggggaa gctataccgg
     1021 cttcggtcgc agcctgctcg atacaacagt ggcggattcc gaccggtatg tcatcaaccg
     1081 catcggcgaa acctgccaga tcatcaccga taaatacatc ctcggcttcg atatcctgcg
     1141 gaataaaagc agctatctct atcattaccc aaccgacagc gcatgcagga aggacctcct
     1201 gaaggagggg gatggcggac ccgcacggag aaaactggag acgctgatcc gcgccaatat
     1261 ccagagctac cgcgcggcac tcacaaaacg aaaactggac taagggaagc cggacgggag
     1321 aaagacgcct atttaccttc cagtttattg caatactcga gggtgcccag ccggaagctg
     1381 accggaagcc ggagcgtttt ctggatagcc agctcctgct tgcagaagaa acccaggtga
     1441 ttcacataat agtccccgcc tacggcgccc agggggccac ccacggcgct gatccggaag
     1501 ggcggtaacg ccgacggtaa agcaggttta acaggaaaca acgggaagac cggcttgccg
     1561 gcaggtaaaa acaccggcga aaaaataatt ttttccaccg ggaggggcgc ttttgactgg
     1621 gcgcttgcca ctccggtaag tattgaaata catacaacaa gaaaagtttt ttttcgttgc
     1681 acggttggtc cctccttttt ccttagattt acggcctgat ttttgtggtt cgcccgcctt
     1741 tttcccggta acaggagaaa ggggtagagc aaaacccctg atatacatgt cgttaagtca
     1801 atcattacag caaaaattac tccaaaaact gtctcctcag cagattcagt taatgaaatt
     1861 gctgcaggta ccaacggcca acctcgaaga acggatcaag gaggagctcg aagagaaccc
     1921 ggctcttgaa gtgacggaag accacgaaga tggggaagaa acaaaggatg agttcgattc
     1981 ggcggaggaa tttgaggagg cggatggcag cgaggacgag tatgacaaca tcgacatcag
     2041 cgaatatgtc aacgatagcg atgacgagat cgcagattac aagctccgcg acgacaatta
     2101 cccggaagcc gacgaagatc gggtaatgcc ccaccgggtc gaaacatcgt tccatgagct
     2161 cctgctggat cagctgggca tgctggtggt cgacgaccgc acccggaaga tcgccgaaca
     2221 gatcgtcgga agcatcgacg acgatggcta cctgcgccgc gaaacggcat ccatcgccga
     2281 tgacctcgcc ttccgccaga atatcgaaac atccgaagaa gagatagagt cgctcatcaa
     2341 aaagatccag cagttcgatc cccccggcgt cgctgcccgc gatctgcagg aatgcctgct
     2401 gaaccagctg cgccggaaga agaacgggaa caggagcgtc gatacggcga tcgacgtact
     2461 cacctattac ttcgacgaat tcacaaagaa acactatgac aagatccagc ggggactcaa
     2521 tctcagcgac gaccagctga aggaggttat caaccagatc atcaagctca accccaagcc
     2581 cggcggcgac gtcaccgagg tgaacaaggc ggaaagctat gtgatccccg acttctttat
     2641 cacgaacagc gccggcaaac tggagctctc cctgaactcg aagaacgccc ccgacctgcg
     2701 catcagcgaa ggatacaagg agatgctgaa ggaatacgac cggggcagta aaaaggacaa
     2761 acgccagaag gaggcggtgc tctttatcaa acagaagata gacgcggcaa aatggttcat
     2821 agatgcgatc aaacagcggc agcagaccct gtacaacacg atgcatacga tcatggacta
     2881 ccagcacgac ttcttcctca caggggatga aacgaccctc cgcccgatga tcctcaaaga
     2941 catcgccgaa cggacggccc tggatatctc caccgtcagc cgggtcgcca acagcaaatt
     3001 cgtccagacg gagttcggaa cctaccggct gaagttcttc tttagcgaat ccctgagcac
     3061 cgacagcggg gaagaagtat ccacccggga agtaaagaag atcctcagcg atctcatcga
     3121 aggagaaagc aaaaagaaac ccctgagtga cgagaaactg accgaactgc tgcaggagaa
     3181 agggtataac atcgcccgcc gcaccgtagc caaataccgc gaacaactaa acattcctgt
     3241 cgcaagactc agaaaggaat tatgatcaaa aaagcgttag cgcagttttt ttcgattgtc
     3301 tttcacccgc tgttcatcag cgcctatgtg atggccttta tcatttttgt ccatccctat
     3361 gcctttgccg gcttcgagca caggcagaag gtgatgcggt tgctcaccgt cgtcctctgt
     3421 aatacggcgc tgcccctgtt cgccgtcttc ctgctctggc gcctgcagtt gataaaatcc
     3481 ccgatgctgc ggaacgaaaa ggaaaggatc gggccctacc tgatcgccat gatcttttat
     3541 ttttggacgg cctatgtgtt caaacggctc cccgatacgc cgcccctcgc cgtccacttc
     3601 ctgggcggag ccttcctggc ggtatgcgcg gcctgggtct gcaatatcta tttcaagatc
     3661 agcatgcacg cgatcgccat gggcggagca acgatgttct tttgcttatt cggccttacc
     3721 gacgagtttg gctccgggct ctacatcgcc gcagccctcc tgctcaccgg cctcgtctgc
     3781 acctcccggc tgctcctttc cgcacacagc tccttcgagg tctgggccgg gatctttatc
     3841 ggcctgttca cccagttcgc ggcctggcaa tggccctggc tattgttctt ttaagagaaa
     3901 ggataataga aaaagaaaag cccaaccata gggtacgaac ctatattgag ctttttcacc
     3961 ttcaaaccat ctgaagtgcg tccatgctag ctgttaagta tcttcgatcg ggcggtgtat
     4021 tttttggttg ggtgataaat aaataaacag gaaccttctt ttataagatc gataattgtc
     4081 tccgattcat ctgccggtac ggccgggcaa ccataacttc ttccacagaa ctggttcatc
     4141 tccaaaaaat ccggtcctac atactggctg ccatgcacta cgatattcct tcctccggcc
     4201 ctgtcattga acccccgctc catccccctg atcttcaggg acagcccgtt gttcccaaaa
     4261 tacgttccct gggtgacata aaacccaaga ctgctcttgt gcgaagaagg gttgttggag
     4321 aacgaccggg caaactcctg accagaattc cggccatgcg caacatacgt attcaccagt
     4381 actttccgct gatcgaggtc cagtacatac aaccgcttgc tgcgcgagga ctgactcaga
     4441 tcacagatgg acaaaatatt cgtcttattc aaccagtgat gatccaatag ataataatac
     4501 cctttcagcg catactcgaa tgccttcttc gtcagcccga cttccttcag atcgaactcc
     4561 tcataaagat tggtgacctc cgcggcaaaa gcggcggccc gcgctgaact cttggttttg
     4621 accggaccac cggcccggag cacacttacc ggtaaaacaa atgcgaccaa aacgcaccag
     4681 aatcccggat gaaatagact gcgacgcatg tttcaaaggt ttttttatca gaagccaaaa
     4741 gggtctttta gtcccctccg ccgataaccc cggggtccgg ggtacggacg aagttattaa
     4801 cggaggggaa actaaaatat tatctatttt ctggttgtgc aagaatctat tatgaattat
     4861 ttttattgtg ccccggtcga cttttccaca tattaaatgc ttccacctac acctttaccc
     4921 gcttccagcg gccccggcga aaaacgacaa aactgacaat ggtaatactc gtctccgtga
     4981 taaggatcgc cagaaagacc ccctttggcc ccaatccgag cgcaatagcc agggcccagg
     5041 caaggggaat ttgaaaggcc caaaagcaaa aaagcccaat aatggtaggc gtacgcgtat
     5101 ctccggcccc gttaaaggca ttggtgagca ccatgcctac cccataaaac acatatccaa
     5161 tactgataat gcgtaaggcc ctgatcgcaa cctcctcgac cgccgggtcc cggttgataa
     5221 aagacacgat cggccccgcc acaaagagga aaagcagcga cacgaataac atgaacgccg
     5281 cattgtagat cgacgccgtc cggacggact gctccgcccg ctcaggggaa cccgccccca
     5341 ggttttgacc cacaagcgta gccgcggcat tgctcatccc ccacgccggc agcaggaaga
     5401 aaagcaggat acggatcgac acctggtaac ccgcgatcgc cgccgtatca ttgaagctgg
     5461 caatgatcct cgccatgacg atccacgagg ccgacccgat caggaattgg gtcgctcccg
     5521 tccaggcgat cttgctaagc ccgcggatga tagcgccatc cggcaggaat tgcttccgca
     5581 ccagccggat gatcccactc ccccgcaaca ggtggaacag ctgataacaa accccgacac
     5641 ccctgccgat gatcgtcgcc atcgcggcgc cggcgaggcc gtagaagtgg ataagtatcg
     5701 gacaaaggat gatattgcaa ccattggcga tccagagcga ccacatcgcc atcgccgcat
     5761 tgcccgctcc ccggaagatg ccgttgatca ggtagagcag catgatgacg agggaccccg
     5821 caaaaacaag ccgggtatag gccgaaccaa tggccacggt cgcggcggaa gcgcccatga
     5881 tcccaaggat atgcggagcg agaaaggcgc cggccacact gatcacgacg gtcaggagaa
     5941 gcgagatgac gatggactgg gcggcagtat gcgaagcctc ctccgggttc ttctcgccga
     6001 tacgccgcgc gaccatcgcc gttgccgcca tgctgagccc gatagcgacg gagtacacca
     6061 gcgtcaggac cgactccgtc agccccacgg tggctaccgc atcctggcca agacgaccca
     6121 caaaaaaaat atcgacgatc gcaaacaccg actccataca catctcgagg atcatcggta
     6181 tcgacagaag gatcaccgca cgccggatgc tgcccttggt ataatccaga tgctgtcctg
     6241 aaagcgactg acgcaggaca agcgcaatag tagaaaaggt attccctttt tgattaggtt
     6301 gtgacgttga cataaagccg ttttagtaaa gctgaaataa taaagattgc cctttgagca
     6361 ggagctcagg aatgcgctgg agggagatct gcaatggaga cagatcgaag cttagcaggt
     6421 cataagcgga gacattagag gataaagatg agaaagtttt ttaataaaat aaaatattcg
     6481 gaccggtttt cgccgggaaa ggggtgtact tttgagccat gagatcggct gcgctgcgta
     6541 tatcactaag agtcctgaag atcgccggca tcacggccgg agggctggtc ctgctgcttt
     6601 tcctcttac
//
LOCUS       DATGFG010001091         5977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_359765, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001091 DATGFG010000000
VERSION     DATGFG010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359765"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VKQ52_20695"
     CDS             <1..242
                     /locus_tag="VKQ52_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89690.1"
                     /translation="HLLLQYDTETRNVYRGLDGKLPEPGQVQNIRLQPLDQLDPATFT
                     LLQGRQDTRLEQLKKAVRLLNQPLEDRPAHHPKLK"
     gene            244..579
                     /locus_tag="VKQ52_20700"
     CDS             244..579
                     /locus_tag="VKQ52_20700"
                     /inference="COORDINATES: protein motif:HMM:NF024960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4133 domain-containing protein"
                     /protein_id="HLZ89691.1"
                     /translation="MATVYSVNRGVNRSLEFKGIKAQYVIYLSLGLVLLLLLFAVFHL
                     AHVNLYVGITIILVSGGVLVLAIKRLSDRYGEHGLIKHGAARRLPASVQSRSRKIFLQ
                     LKPPTSEKA"
     gene            566..3034
                     /locus_tag="VKQ52_20705"
     CDS             566..3034
                     /locus_tag="VKQ52_20705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032991 - protein-containing complex
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraG family conjugative transposon ATPase"
                     /protein_id="HLZ89692.1"
                     /translation="MKKHKTLEKLLPIFKVEQDCILSRLGDITIAYELTLPEIFTLSA
                     DDFEAIHQAWLKAIRVLPNHTVVHKQDWFMENHYQADFSNPDQSFLARSSERFFHERP
                     YLDHHCYLYLCQKPEGRRPSTALFSTLIRPTLVPVSVQDTKRWDLFLDKAGQFARILQ
                     ESGYIQLRRLTNDELASTTDRPGLLERYTYLLHPQEPPVIRDLQLSPDFRIGDLSCQL
                     FTLADVEDLPPLCGPRVNYDKYSTDRTKFSVSFAAPLGQLLSCNHLYNQYIFIEDSRE
                     TLKKMEARRLRLQSLSAYSRENAISRDATNDFLNEAVGNQRLPVKAHFNLLTWSADPV
                     QAKEMKNIVSAALAQIDAHTRQETVSAPQLFWAGMPGNAGELPMHETFDTFAEQAACF
                     LNIETNYRSSISPVGLRLGERLNGRPIHVDISDEPMARGICTNRNKFILGPSGSGKSF
                     FTNLFVRSYVEQGAHAVIIDVGHSYRGLCELLEGYYFTYSESDPIRFNPFFLGEEQLD
                     TEKKESIKTLLLALWKKDDESFSRSEYVSLSNSLQAYFDFLKQHPDIFPCFDSFYEFL
                     RDQFLVTLRQSGIKDKDFDIDNFLYVLRPYYTGGEYDYLLNARQNLDLLAQPFCVFEI
                     DAIKDHPILFPVTTIIVMQLFIDKMRKLKGIRKIILIEEAWKAIMRQGMAEYVKYLFK
                     TVRKFYGEAIVVTQEVEDIISSPIVKQAIINNSDCKILLDQSKYQNKFDQIQEMLGLT
                     EKEKALVLSVNKSNDPKRKYKEVFISLGGMVSKVYRTEVSLEEYLAYTTEEKEKMHVQ
                     AYSKKYGSIRRGIAMLARDMRATS"
     gene            3179..3583
                     /locus_tag="VKQ52_20710"
     CDS             3179..3583
                     /locus_tag="VKQ52_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89693.1"
                     /translation="MKLIILLSIITVLFFSCDGRSKAVPTRNSQPVPAFFVSGIYTSF
                     EKSSYCRTWDTLVVTRDRRQINVYRITRLTSFQRNLEDEYDTIERDSSSWTGSYDPTK
                     AIMHVLDQGQPLIFQPASYTAYLDNHVFTKVE"
     gene            3599..4261
                     /locus_tag="VKQ52_20715"
     CDS             3599..4261
                     /locus_tag="VKQ52_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89694.1"
                     /translation="MKRIRAILVAPLLLLLSCIPATSKAQFTEIIEAVIEAIDVGVQK
                     AQNAVIDLQNAEKEIENDLSQTQLGQIANWTQQQKDLYSNYFTELWKVKTVITDFRKI
                     TSIIANQAQLVSEYKAAYAQVKRDPHFTPSEVTYINNVLTGIINESIQNIDQITKLLE
                     SFTVQMADADRMRLIQHASDNIQREIVNLRAFNNQNAALSLQRATDQNDLNTIKQLYG
                     LP"
     gene            4271..4735
                     /locus_tag="VKQ52_20720"
     CDS             4271..4735
                     /locus_tag="VKQ52_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89695.1"
                     /translation="MSLFADILDVHTNWLTRKGFDQQGLGLIGTNQDLLNSLEDALFH
                     TVCDKNNFWARKPLDIDLKITGHLGRLNKELVNFHFHYVYYPKRPKLNLLALQAEWKG
                     ITNTWLITGDRQHLLPTAEHAYNELYYKANSHRINNAIHISPRTENFTARLH"
     gene            4784..5425
                     /locus_tag="VKQ52_20725"
     CDS             4784..5425
                     /locus_tag="VKQ52_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89696.1"
                     /translation="MLTLKIHMPRIRNIAILMFTFVLAAHPSKAQMDRDEMLAQIAKL
                     QVFISQAEKGYQLVREGLTTIGDIKKGDFDLHQLFFTSLQLVSPEIRSYVKIADILAM
                     HANMLSAYHSGYNQVKTAGLFSASDITYINSLYQGILGKSSDDIQELTGILTDGTWQM
                     NDAQRISRIDHLYESVTAQYQSLIGFSDRLQLLALQKRQSLQDLQNITQLIRP"
     gene            5422..>5977
                     /locus_tag="VKQ52_20730"
     CDS             5422..>5977
                     /locus_tag="VKQ52_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89697.1"
                     /translation="MKKCFLLPLIFLIVTFAPKRASCQAAQLEQLALDIEKLAQFKSI
                     LADMKKGYQILTNGYNTVRNLAQGSFDLHQAFLNSLLTVSPTIKNYVRIADIISTQAA
                     IVSEYKHAINQFKASGMLSPSDLNYLAGVYSNLFDHSLDNLDVLVSILTDGQLRMSDA
                     ERLASIDAIDADMHSQLNFQRSFLG"
BASE COUNT         1606 a         1738 c         1207 g         1426 t
ORIGIN      
        1 atcacctctt actccagtac gacacggaga cccgtaatgt ctatcgcggc ctagatggga
       61 aactacccga accgggccag gtgcaaaata tcagacttca gccgctcgat caactcgatc
      121 ccgccacttt tactctgcta caaggccggc aagacacccg gctcgaacag ctcaaaaagg
      181 ccgtccgtct gttaaaccag ccactagaag ataggcccgc ccaccatccc aaactcaaat
      241 aacatggcca ccgtttattc tgtcaaccga ggcgttaatc gcagcttaga gttcaaggga
      301 atcaaagcac aatatgtcat atacctttcc ctcggtcttg ttctcctgct tctccttttt
      361 gctgtctttc atcttgctca tgtcaatctc tatgtcggca tcaccatcat tttagtctcc
      421 ggtggagtac tcgtactcgc gatcaagcgg ctcagcgacc gatatggtga gcatggatta
      481 atcaaacacg gcgccgcccg ccgccttccc gcctctgtcc agtctcgttc caggaagata
      541 ttcctccagc tcaaaccgcc gaccagtgaa aaagcataaa acgctcgaaa agctcctgcc
      601 gatctttaag gtcgagcagg attgtatcct ctcccgcctt ggcgacatta ccatagctta
      661 cgagctaaca ctgccggaga tattcactct atccgcggac gatttcgaag ccatccacca
      721 ggcctggctc aaggccatcc gcgtgctccc gaatcatacc gtggtgcaca agcaggactg
      781 gttcatggaa aaccattacc aggccgactt ttccaacccg gaccagtcct ttttggcccg
      841 tagcagcgaa cgcttcttcc atgaacggcc atacctcgac catcactgct acctttacct
      901 ctgccaaaag cccgaagggc ggcgtccatc caccgccctt ttttctacgc tgatccggcc
      961 caccctagtc ccggtctccg ttcaggatac taagcgctgg gacctctttc tcgacaaagc
     1021 cggacagttc gcccgcatcc tccaagaatc cggatatatt caattgcgcc ggctgaccaa
     1081 cgacgaacta gccagcacca ccgatcgacc tggattactg gaacgctata cctatctcct
     1141 ccatccccag gaacctcccg tcatccgcga ccttcaactc agtcctgact tccggatcgg
     1201 cgatctcagc tgccaactat ttacccttgc tgatgtcgag gatcttccgc ccctctgcgg
     1261 cccgcgggtc aattacgaca agtactcgac cgaccgcaca aaatttagtg tcagctttgc
     1321 cgcacctctc ggtcagttgc tttcttgcaa ccacctgtac aaccaatata ttttcatcga
     1381 ggattcccgc gagaccctca aaaagatgga agctcgacgc ctccgcctcc aatccttatc
     1441 tgcctactcg cgcgaaaatg ctattagccg cgatgctacc aatgatttcc ttaacgaagc
     1501 cgttggtaac caacgtctgc ccgtcaaagc acactttaat ctgctcacct ggtcggctga
     1561 tcctgtacag gccaaagaaa tgaaaaatat tgtctccgct gcccttgctc aaatagatgc
     1621 ccatacccgg caagagactg ttagcgcgcc acagctcttc tgggctggca tgcctggtaa
     1681 cgctggcgaa ctacccatgc atgagacctt cgatactttt gctgaacagg cggcttgttt
     1741 cttgaatatc gagactaact atcgcagctc catctctcct gtgggtcttc gcctcgggga
     1801 acgccttaac ggaagaccga tccatgttga tatttctgac gaacctatgg cgcggggcat
     1861 ttgcactaac cgaaacaaat ttattctcgg gcccagcggc agtggaaaaa gctttttcac
     1921 caatttattt gtccgaagct acgttgagca gggtgctcac gccgtcataa tcgacgtcgg
     1981 gcacagctat cgaggcctat gcgaattact cgaaggctac tactttacct actcagaatc
     2041 cgatcccatc cgtttcaacc cctttttcct aggggaagaa cagctcgaca cagaaaaaaa
     2101 agaatcaatt aaaacgctgc tgctcgcact atggaaaaaa gatgatgaaa gcttcagcag
     2161 gtccgaatac gtctccctct ccaattcgct ccaggcatat ttcgatttct taaagcaaca
     2221 cccggacatt tttccatgtt tcgatagctt ctatgagttc ctccgagatc aattcctggt
     2281 cacccttcgt cagtccggca taaaagacaa agacttcgat attgataact tcctctatgt
     2341 tcttcgacca tactataccg gaggcgaata cgactattta cttaatgccc gccagaatct
     2401 cgaccttctc gctcaacctt tctgcgtatt cgaaattgac gcaatcaaag atcatccgat
     2461 tttgtttcca gtaacaacca tcatcgtcat gcagctcttt attgacaaga tgcggaagtt
     2521 aaagggtatc cgaaaaatta tcttgataga agaggcgtgg aaggcaatca tgcgccaagg
     2581 catggccgaa tatgtgaaat atctatttaa aacggtccga aaattctacg gggaggccat
     2641 cgttgtaacg caagaggttg aagatataat ctccagtccc atcgtcaagc aagcgatcat
     2701 aaataatagt gattgcaaga tcctgctcga tcaatctaaa taccaaaata aatttgatca
     2761 gatacaggag atgcttggac tcaccgagaa ggaaaaggct ctagtccttt cagtcaataa
     2821 atccaacgat ccaaaacgaa aatataaaga ggtgtttatt tcccttggag gcatggttag
     2881 taaggtctat cggaccgagg tctcactgga ggaataccta gcatatacca ctgaagaaaa
     2941 ggaaaaaatg catgtccagg cctattcgaa aaagtacggt agcatccgtc gcggcattgc
     3001 gatgcttgct cgcgacatgc gtgccaccag ttgatccaaa ttcttttggt cggccttacc
     3061 ggaatgaatg attttttcac ccaaccacac ctccatactg aaaacccgga tttacagcga
     3121 ccgatctttg ctcaattgag atgacccagt gcctttctaa ccgccaaaat aaattatcat
     3181 gaaactgatt attctccttt ccatcatcac ggtgctgttc ttctcctgcg acggccggtc
     3241 caaagctgtg ccaacccgca actctcagcc cgttcccgcg ttttttgtgt ccggcatata
     3301 cacgagcttt gaaaaatcca gctattgccg cacatgggat acgctcgtag tcaccaggga
     3361 ccgccgccaa atcaacgtct accgcattac tcggctcacc tcattccagc gtaacctgga
     3421 ggacgaatac gacacgatcg aacgggacag ctcgtcctgg accggatcct atgatccgac
     3481 gaaagccatc atgcacgtcc tggaccaagg gcagcccctg atcttccagc ccgcatctta
     3541 taccgcatat ctcgacaatc atgtctttac taaggttgaa taaatcaata cctacccaat
     3601 gaaacgtatc cgagccattc ttgttgcgcc gctgctgctt ctcctttcct gcatcccggc
     3661 caccagcaag gcccaattta ccgaaatcat cgaagctgtt atcgaagcta tcgacgtcgg
     3721 agttcaaaaa gcccagaacg ccgttatcga cctacaaaat gcagaaaagg agatcgagaa
     3781 cgacctgtca cagacccaac ttggccagat tgctaattgg acccaacagc aaaaggatct
     3841 ttattcaaac tacttcactg aactctggaa ggtgaagacc gttattacgg atttccggaa
     3901 aatcacctcc atcattgcaa accaagccca gcttgtatcg gaatacaaag ctgcttatgc
     3961 tcaagtgaag cgggatcctc attttacccc aagcgaggtg acctacatca ataacgtgct
     4021 tacaggcatc attaacgaaa gtattcaaaa cattgaccaa atcactaagc tccttgagtc
     4081 attcacagtc caaatggccg acgcggatcg aatgcgcctt attcagcatg cctccgacaa
     4141 tattcaacgg gaaatcgtca atctgcgagc atttaacaat caaaatgctg ccctaagcct
     4201 ccagcgggcg accgatcaaa acgacctcaa cactattaag cagctctacg gtctccccta
     4261 aacatcagtt atgagtctct ttgctgacat actagacgtg catacaaact ggcttactcg
     4321 gaaaggcttc gaccaacagg ggctcggact tattggtacg aaccaagact tgctgaatag
     4381 tcttgaagac gccctatttc acactgtttg cgataagaat aatttctggg cgagaaagcc
     4441 attggatatc gacctgaaga ttaccgggca cttaggtcgg cttaataagg aactagtaaa
     4501 tttccacttc cactacgttt actaccctaa acgtccgaaa ctgaacttgc ttgccctgca
     4561 ggccgagtgg aaaggaatta cgaatacctg gctgatcacc ggcgatcgcc aacatctctt
     4621 gcccacagca gagcacgcct acaacgagct ttattacaaa gccaacagtc atcgaatcaa
     4681 caacgcaatc catatcagtc cacggactga gaacttcacg gcccgcttac actaagttct
     4741 acgttaggct gccctgcgcc gcccttaata aatccgttcc gctatgctca ctcttaaaat
     4801 acacatgcct cgaatccgga acatcgccat tctcatgttc acctttgttc tggccgctca
     4861 tccgagcaag gcccaaatgg accgagatga aatgctcgcc cagatagcga aactccaggt
     4921 ctttatttcc caggctgaaa agggctacca acttgtccga gagggcctga ctaccattgg
     4981 cgatataaaa aagggagatt tcgacctcca ccagttgttt ttcacttctc ttcagctggt
     5041 gagccctgag attcgcagct atgtcaaaat agccgatatc ctagcgatgc acgccaacat
     5101 gctctcagcc tatcactccg gatacaacca agtcaagact gctggactat ttagcgcatc
     5161 cgacatcacc tatatcaatt ccctgtatca aggtattctc ggaaaaagca gcgatgatat
     5221 ccaggaactc acaggtatcc tcaccgacgg cacctggcaa atgaacgacg cccagcgcat
     5281 tagccgaatc gaccacctct acgaaagtgt cactgcccaa taccagtcac taataggatt
     5341 cagtgatcgt cttcaactcc tagcgctaca aaagagacaa agcctccagg atctacaaaa
     5401 tattactcaa ctcattcgcc catgaaaaag tgctttttac ttccgctcat ttttctcatc
     5461 gtcacgttcg ctccgaagcg agcctcctgt caagctgccc agctggaaca acttgccttg
     5521 gacattgaga agcttgccca attcaaaagc atccttgctg atatgaaaaa gggctatcag
     5581 atcctgacta acggatacaa cacggtccgg aacctcgctc aaggcagctt tgatctgcat
     5641 caagccttcc tcaacagcct cctcacggtc agcccaacga tcaaaaatta tgttcggatc
     5701 gctgatatca tcagcaccca agctgctatc gtctcggaat acaaacacgc tattaatcag
     5761 ttcaaggctt cggggatgct cagcccctcc gatctcaatt acctcgccgg ggtctatagc
     5821 aatcttttcg atcactcact cgacaatctc gacgttctgg tgagcatcct gacagacggc
     5881 cagctccgca tgagcgacgc cgaacgccta gcttctatag atgcgatcga cgctgacatg
     5941 catagccagc tcaacttcca acgcagcttc ttgggcc
//
LOCUS       DATGFG010001092         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1663027, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001092 DATGFG010000000
VERSION     DATGFG010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663027"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VKQ52_20735"
     CDS             <1..419
                     /locus_tag="VKQ52_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89698.1"
                     /translation="ELLNLQQLLNSSELEAPSLRFTRNVMEEIARYHVAPATKSYVNK
                     NVIRGIGAFFLVMILGMMTWLFSQFKWTSGSGSGSGGSSFSLPIDKLGLNRLDNETVS
                     KAFNGTTITLFMLVTVVLGFILLDMYLQQKKQSRIS"
     gene            584..>2491
                     /locus_tag="VKQ52_20740"
     CDS             584..>2491
                     /locus_tag="VKQ52_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89699.1"
                     /translation="MRYLLRAAFFCLSLLSAAFALHAQSLEPFSNCPGVSVAITRPGL
                     NAGLPPFQIYLIDSAGGIKPSGNPIDLQINGFGLDNTDGFLYGIHESSNVANPFLARV
                     DKNGNFQNVGTLLPPAAAPFHVGIINTAAGTSDDKDNYYFLALVANLQNTLEPPVLFV
                     GRIANVSKLSPTDKPLDVKYTPINPGTCLPELLAALANPLSGILQDIAYDPNNGNIYS
                     YIRAPGNDPTPGLLAWFNPADSPRFTCMMPAQANIPTHDLAGLYFGSDSSLFILTTDG
                     KYYRGNVNTGMISLVTQTSLPLMNGNLRGDMASCAGKKPLVAFDNCPGVSVAVTRTGI
                     NSTNSPYQIFTIDKSGNIQPTGNPIPLQINAFGLNNKDGFLYGLHETDSVNDPWFTRV
                     DKFGSLVNIGRLTPPPANGRNVSIINTAAATMDGEDNYYFTAVTTDTPLSPLSIPRLF
                     LGTIHKVSGLKEGDSIPIQYKEVFIGTCVVEILTGLLNRSEGLLQDIAFNPVNHNIYT
                     TFPTKGTSPAPARIGHFNPFAKFPTLNCITPEHPNVPMGDMCGLFSGDGGNLFILTTD
                     GKFYKGNVSNGEIRLITQTALPLLGNNLRGDMASCVQKSDRDDDDHDGQNDNDGSRTE
                     DLTIAPNPIASG"
BASE COUNT          592 a          828 c          553 g          518 t
ORIGIN      
        1 gggaactgct gaacctgcaa cagctcctca acagctcgga actggaagcc ccctccctgc
       61 gctttaccag gaatgtcatg gaagagatcg cccgttacca cgtagcgccg gccaccaaat
      121 cctatgtcaa taaaaatgtg atccgtggca tcggcgcctt cttcctcgta atgatcctcg
      181 gcatgatgac ctggctgttt tcccaattca aatggacatc cggttccggc tcgggcagcg
      241 gcggcagcag cttcagcctg cccatcgaca aactgggcct caaccgcctg gacaatgaaa
      301 ccgtctcaaa agccttcaac ggaacaacca tcaccctctt catgctcgtc accgtagtac
      361 tgggctttat actgctggat atgtacctgc aacaaaaaaa acagtcgcgt atttcgtagc
      421 ttctagtccg atttttgcgc aaacaggatt aggcaaccgg cggggccatc ccgccgttct
      481 ctattgctga gccccgcctg cccaatgtcg cacttctctc cctaaagcca cgccgtagat
      541 ccggtctgcc cggattatct cctgctaatc aaacgtttgc tatatgagat atcttctacg
      601 cgccgccttt ttctgtctat cactattgtc agccgcattc gccttgcacg cccaatcatt
      661 ggaacccttt tccaactgcc cgggagtgtc cgtcgccatc acccggcccg gcttgaatgc
      721 aggcctcccg cccttccaga tctacctgat cgactccgca ggaggcataa agccttccgg
      781 caatcccatc gatcttcaga tcaatggatt cggactggac aacaccgatg gcttcctcta
      841 tgggatccac gagtcctcga atgtcgcaaa ccccttcctg gcaagagtgg ataagaacgg
      901 gaattttcag aacgtaggca cgctcttacc ccctgccgcg gcacccttcc acgtgggcat
      961 catcaatacg gccgccggta ccagcgacga caaggataat tattattttc tcgcgctggt
     1021 cgccaatctg caaaacacac tggagccccc cgtcctgttc gtaggcagga tcgccaacgt
     1081 ttcaaagctt tccccaaccg acaaaccact cgatgtaaaa tataccccca taaatcccgg
     1141 cacctgcctg cccgaactcc tcgccgccct ggctaatccg ctgtccggaa tcttacagga
     1201 catcgcctac gatcccaata acggcaatat atacagctat atccgcgcac ccggcaacga
     1261 ccctaccccc ggactgctcg cctggttcaa tcccgccgat agcccccgct tcacctgcat
     1321 gatgcccgca caggccaata tccccaccca tgacctggcg ggactttact tcggcagcga
     1381 tagctccctg ttcatcctta ccaccgacgg aaagtattac aggggcaacg taaacaccgg
     1441 catgatcagt ctcgtaaccc aaacctcatt acccctcatg aacggcaatc tgcggggaga
     1501 catggcctcc tgcgcaggta aaaaacccct ggtcgccttc gataactgcc ccggcgtatc
     1561 ggtggccgtc acccgaacgg gcatcaacag caccaatagc ccctaccaga tctttaccat
     1621 cgacaaatcc gggaacatac agccgacagg caaccccata ccactgcaga tcaacgcctt
     1681 tggcctgaac aacaaagacg ggttcctcta cgggctccac gagaccgata gcgtgaacga
     1741 tccctggttc acccgcgtag acaaattcgg cagccttgtg aacatcggca ggctcacacc
     1801 tcctcccgcc aacggccgca acgtatcgat catcaatacc gccgccgcca ccatggatgg
     1861 tgaggataac tattatttca ccgccgttac caccgatacc cccctgagtc cgctgtcgat
     1921 cccccggctc ttcctgggta cgatccacaa agtatcgggt ctgaaagaag gggattccat
     1981 acccatccaa tacaaagagg tcttcatcgg cacctgcgta gtggagatcc tcacaggctt
     2041 gctgaacagg tccgaaggcc tcctgcagga tattgccttc aaccccgtca accacaatat
     2101 ctacaccacc ttcccgacaa aaggaacatc gcccgctccg gcccgcatcg gccatttcaa
     2161 tcccttcgcc aaattcccca ccctgaattg tatcacgccg gaacatccga atgttcccat
     2221 gggggatatg tgtggcctct tctccggtga tgggggcaat ctcttcatcc tcaccaccga
     2281 cggaaaattc tataagggga atgtcagcaa tggagagatc aggctgatca cccagacggc
     2341 cctgccgctc ctggggaaca acctgcgtgg cgatatggct tcctgtgttc agaaaagcga
     2401 cagggatgac gatgatcatg acggacaaaa cgataacgat gggagtcgca cggaagatct
     2461 gaccatcgcc ccgaatccga tcgcttcggg t
//
LOCUS       DATGFG010001093         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_396203, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001093 DATGFG010000000
VERSION     DATGFG010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396203"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VKQ52_20745"
     CDS             <1..492
                     /locus_tag="VKQ52_20745"
                     /inference="COORDINATES: protein motif:HMM:NF027591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4919 domain-containing protein"
                     /protein_id="HLZ89700.1"
                     /translation="LYYGFVFQKEYSPYGDDRKGAILGFMRDKKYPVVIAVCDSILER
                     TPVSLVGNYYRGLAMYLEGKDNPAVVDSAYKGFRDRFVGLQKAILSTGDGLSCKTAFK
                     TIFVSDEYEIVSRWFELDAPSGQSLVYPCDKLAVRPNAYFKSNAIYFDTSETFIAMKN
                     KFK"
     gene            complement(567..752)
                     /locus_tag="VKQ52_20750"
     CDS             complement(567..752)
                     /locus_tag="VKQ52_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89701.1"
                     /translation="MQHIIKNLLSAALFIGVTAFALSGCYETHYYHHYHHHTHEWYDR
                     HHTPPPAGVDFNVDIHN"
     gene            complement(1030..1779)
                     /locus_tag="VKQ52_20755"
     CDS             complement(1030..1779)
                     /locus_tag="VKQ52_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89702.1"
                     /translation="MFGLFKKDAAPESPLKDHRRQWVEEAFQQLTRTFHRQSILVRKV
                     LTPHYSDFPIRYNGDPQTARDTLDIVVAQMEIDPTEIELSFYDDTINQVSTGSPSGGK
                     LTMSPADGEQPSDEPIYTKKEEGKYPVTVRTSRLQSPEAMVATLARELAYIKLLGESH
                     ITEPDAALIDLTTVIFGLGIFNANAAFTSQNLRITGKNARLTQMEWGYGLALFAHLRK
                     EKKPAWIDHLVRNIKSDFLKSEQYIAYHNPQ"
     gene            complement(1846..>2477)
                     /locus_tag="VKQ52_20760"
     CDS             complement(1846..>2477)
                     /locus_tag="VKQ52_20760"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HLZ89703.1"
                     /translation="PYTDIAVDYLLDQQRPDGTFKAEGGRPPHETGEAHLAAMCIYAI
                     QLYASPAKKARVDKAISRTKQWLAGYQPNNQQELVFQILGLHWTGASIAGKEKIAARL
                     IAMQNSDGSWSQLPTMHGDAYATGEALYALGESGVTRPEEECIQKSISWLLKHQDPSG
                     AWIVETRAYPLQSFFNCGFPPYDENQFISAAATSWAGLALLTSLPDDLK"
BASE COUNT          507 a          545 c          764 g          661 t
ORIGIN      
        1 ctttactatg gatttgtgtt ccaaaaagaa tatagccctt atggggatga tcgtaaaggc
       61 gcgatcctcg ggtttatgcg ggataagaaa tacccggtgg tgatcgccgt gtgtgacagt
      121 attttggagc ggacgccggt gagtcttgtg ggcaattatt accgggggtt ggcgatgtac
      181 ctcgagggga aggataatcc ggcggttgtg gattcggctt ataagggatt ccgggaccgg
      241 tttgtgggat tgcagaaggc gatcctgtcc acgggggatg ggcttagctg taagaccgct
      301 ttcaagacca tctttgtgtc ggatgagtat gagatcgtgt cccgatggtt cgagctggac
      361 gcgcccagtg ggcaaagcct ggtctatccc tgtgataaat tagctgtcag gcccaatgcg
      421 tatttcaaat ccaacgccat ctattttgat acttcggaga cttttattgc gatgaagaat
      481 aaatttaaat agggcaaaaa agcgacccgg gagcgttgcc cccgggtcta accatgaacc
      541 gtcccgatga ataagattga gctcgtttag ttgtggatat cgacgttgaa gtcgacgccg
      601 gcgggtggcg gggtatggtg gcgatcgtac cattcgtgtg tgtggtgatg gtagtgatga
      661 taataatggg tttcgtaaca gcccgacagc gcaaaggcgg tcacgccgat aaagagggct
      721 gcactgagaa ggttttttat aatatgttgc atgacgcgct gtttccatta tagtatcgaa
      781 aaaccttgcc agccttggta aaggggggaa aagcggcttt ttttcctgca agaagggaag
      841 gactgtagaa aaaattccac caaagtggta tttcacgggt ggaccccggg ggctattttt
      901 gtgtaccgtt gctgacctat caaaatgaat tacggacggt tttcactttc agccatcggc
      961 ggatcctctt ttatagggga ccgcctttct ctttttaggg gggattgggt agaataggag
     1021 gtatttgagt tattgagggt tgtggtaggc gatgtattgc tcgcttttca ggaaatccga
     1081 ttttatattc ctgaccaggt ggtctatcca ggctggtttt ttctccttgc ggagatgggc
     1141 aaagagcgcg aggccataac cccattccat ttgggtgagg cgggcattct taccggtaat
     1201 ccggaggttt tggcttgtga aggcggcgtt ggcgttgaag atgccgagcc cgaaaatcac
     1261 ggtagtaaga tcgatgaggg ccgcgtcggg ctccgtgatg tggctctcgc ccaatagttt
     1321 tatatacgcc agctcgcggg cgagcgttgc caccatggct tcggggctct gtaacctgga
     1381 tgttctgacc gtgaccggat atttgccttc ttccttcttt gtatatattg gttcgtcgct
     1441 tggctgttcg ccatcggctg ggctcatggt cagcttgccg cctgaggggg acccggtgga
     1501 aacctggttt atcgtatcat catagaagct tagttcgatc tctgtggggt cgatctccat
     1561 ttgcgccact actatatcga gtgtgtctct tgccgtttga gggtcgccgt tgtaccggat
     1621 agggaaatcc gagtaatggg gtgtcaggac cttgcggaca aggatggatt gccggtgaaa
     1681 tgtccgggtg agctgctgga atgcttcttc aacccactga cggcggtggt cctttagcgg
     1741 gctttccggt gcggcgtctt ttttgaatag accaaacata gcgtgacaag ataggatttg
     1801 ccgcggaagc gtaaacgcag ggtgggaact tagcggcgat ggcttttact tcaggtcatc
     1861 cggcaggctt gtcagcaggg cgagaccggc ccagctggta gccgcggcgg agatgaactg
     1921 attctcatcg tagggtggaa aaccgcagtt aaagaaggat tgaaggggat aggcgcgggt
     1981 ctccacgatc catgcgccgc tggggtcctg atgtttcagc aaccaggaga tgcttttttg
     2041 aatgcactcc tcttcgggcc gggttacgcc gctttctccc aatgcatata gggcttcacc
     2101 agtggcgtat gcatcgccgt gcatcgtggg cagctgagac cagctgccgt ctgaattttg
     2161 cattgctatc agccgggcgg ctattttttc tttcccggct atcgaagcgc cggtccaatg
     2221 gaggcccagg atctggaaga ccagttcctg ctggttattg ggttgatagc cggcaagcca
     2281 ttgttttgtg cggctgatgg ccttgtccac gcgggctttt tttgccgggg atgcatagag
     2341 ctggatggcg tatatacaca tcgccgccag atgggcttcg ccggtctcat gcgggggccg
     2401 gccgccctcg gccttgaagg tgccatcagg ccgttgttga tcgaggagat agtctacggc
     2461 aatatcggta taaggat
//
LOCUS       DATGFG010001094         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1318002, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001094 DATGFG010000000
VERSION     DATGFG010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318002"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..>3654
                     /locus_tag="VKQ52_20765"
     CDS             391..>3654
                     /locus_tag="VKQ52_20765"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HLZ89704.1"
                     /translation="MATKRVVFFSALFLLVFIVTGNAQPNPDKYTAKWRSIDSLISRK
                     GLIESALAEVNTLGALARQEKNKPQEIKALVYRLSLGQARTDSGTLFAIGHLEKELAA
                     QDRQPARAILQCMLAGLYQRYIGENRYAFMGRTNTSGFVKTDVATWSTDDFVKKIHGL
                     YQEALADEDLLWRTPVDQYSPILIKGNTPALRPTLFDLLAHTALEYFTMGESIIQPSV
                     TPTDSLSSHKQALLLFQRLIRRHLTDKTPDALIDIDLQRIGFIHQYSVGVDKEEEYMR
                     MLEAVTDKYGTLPAAAEAWYLQAEHYALQANPSLDQRDSTGNRKAVAICEKVLTQKDS
                     SEGRAHCERLLYNLRQQDLDLTMEKVNIPNRPFRVLVNWRNFPRLYLRLIRVDDPMSE
                     PAYDAYDSMFWKKMLRRPVYRAWGQDLPETKDYTLHRVEVAIGSLPPGSYILLASTNA
                     NWELGGATMGIREIAVSNISYISQENDYYVLNRETGAPLADATVQLWEERGGNRTSWK
                     KGDSFTTDGQGHFLLKRKNTNDDRQVRLEIMTPGDHLYPTDEFYYYYRGGRGKEMVLT
                     GEKYEARQAHSYLFTDRSIYRPGQTVYFKGIELTKDADSHKDKPRAGVTIRVTLVNAN
                     GQKVDSLQLTTNEFGSYNGTFRLPEQQLNGSFSIQEDSSRGSTSFQVEEYKRPKFYVE
                     YGKQQGSYRVGDSIRVSGFAKGYSGNGIDGAKVVYHITRRAHFRYPWLIWRRPMPAGE
                     AEIGHGEVLTDAQGQFKLRFYALPDQQISPATKPEYDYEISADVTDINGETRNGKTTL
                     VAGYTAIRLTIDPAGGDHLPVDSLRDIAVSATNLSGEPVGADVGLLIYPLQAPQRLIR
                     QRLWEAPDRYILPETDFLDSFPHDEYREESKKESWEKGKPLWEKSWPIGDSGKARVVL
                     PAGLLTTGWYLIEVAGTDKYGQRVTDRRYVNLVDGKTGRPANPEYEWTSDGDVRAEPG
                     GKVAVTGGSSAGDVFVIRTVERTEGNEYGHFTLNREGKNTEWTITEADRGGFRVRDAF
                     VKDNRLYTHYTTVHVPWTNKELSIHYGTYRDKTLPGSGEKWQMTISGSKGEQVSAEVL
                     "
BASE COUNT          872 a          918 c         1135 g          729 t
ORIGIN      
        1 gatcacgacg atcgtgataa agatcgcggc gatcgtaatt ccggtcgtac cggagatcgg
       61 cacggtcatg gcggagatcg gcgcggtcgt gacgaacctc catccggtca tgatttatat
      121 cccgacgatc ctgagcctgg gtgcttacgg cgaagaaaag gccggcggcg atgagtaggg
      181 tcaatttcgt tttcatacat gtaaatttta agttttgtcg agctcaatag cgaactcata
      241 tataagatgc cggacaaaga gttgtgttta agggagatac gttatagtcg tgttaaagca
      301 ggcatttttt tgtgtaatcg gctcacccac cacatcttat acaaggacag taaacggtta
      361 agaaacgtaa ctttaaaaaa tttctctcct atggccacca aacgggtagt atttttctcc
      421 gcgcttttcc ttctcgtctt tatcgttacc ggcaacgccc aaccgaaccc cgacaagtat
      481 acggcgaagt ggagatccat cgattcattg ataagcagga agggactgat cgaatcggcg
      541 ctggcggaag taaataccct tggcgctctt gcgcggcagg aaaagaataa accccaggag
      601 atcaaggcgc tggtctaccg gcttagtctc gggcaggcac gaacagatag cgggacgttg
      661 ttcgcgatcg gccacctgga gaaggaactg gccgcgcagg accggcagcc ggcgcgggcg
      721 atcctgcaat gcatgcttgc ggggctttac cagcgctata tcggggagaa ccggtatgca
      781 ttcatgggca ggacgaacac cagcggtttt gtcaagacgg atgtcgccac ctggtcgaca
      841 gacgacttcg ttaaaaagat ccatgggtta taccaggagg cgctggcgga tgaagacctg
      901 ctttggcgga caccggtgga ccaatactcc ccgatcctta taaaagggaa tactcctgcc
      961 cttcggccga ccctgttcga tctgttggcg catacggcat tggagtattt cacgatgggg
     1021 gagtcgatca tccaaccgtc ggtgacgcca accgactcgc tctcatcgca taagcaggcc
     1081 ctgctcctgt ttcaacggct tatccggcgg catctcaccg ataagacacc cgacgcgctg
     1141 atcgatatcg atctgcaacg gatcgggttt atccatcaat atagcgtcgg ggttgacaag
     1201 gaagaggagt atatgcggat gctggaagcg gtgaccgata aatacgggac cctgcctgcc
     1261 gccgcggagg cctggtacct gcaggcggag cactatgccc ttcaggccaa tccatccctc
     1321 gatcagcgtg acagcaccgg taatagaaag gcggtggcca tctgtgaaaa ggtgcttaca
     1381 caaaaagaca gcagtgaggg ccgggcgcat tgtgaacgcc tgctctataa cctgcggcag
     1441 caagacctgg atctgacgat ggaaaaggta aatattccca accggccgtt ccgggtgctg
     1501 gtgaactggc ggaatttccc acggctttac ctgcggctta tccgggtgga tgatcccatg
     1561 tcggagccgg catacgatgc ctatgacagc atgttctgga agaaaatgct gcgccggccg
     1621 gtctatcggg cctgggggca ggacctgccg gagacaaaag attatacgct tcaccgcgtg
     1681 gaggtcgcca tcggatccct gccgccgggg agctatatcc ttctcgcttc cacgaatgcc
     1741 aattgggaac tgggcggggc aacgatgggt atccgggaga tcgccgtttc caatatcagt
     1801 tatatcagcc aggagaacga ttattatgtg ttgaaccggg agaccggtgc gccgctggcg
     1861 gacgctaccg tacagctctg ggaagagagg ggcggcaacc ggacatcctg gaaaaaaggc
     1921 gattccttta ccaccgacgg gcaggggcat ttcctgctga agcgaaagaa tacaaacgac
     1981 gatcggcagg tccggctgga gatcatgacc cccggggatc atttgtaccc aacggacgag
     2041 ttctattact attatcgtgg cgggagggga aaggagatgg tgctcacagg ggaaaagtac
     2101 gaggcccggc aagctcattc ctatcttttt acagaccggt ccatctaccg gccgggccag
     2161 accgtttatt tcaagggtat cgagctgaca aaggatgccg acagccacaa ggataagccc
     2221 cgggccggcg taacgatcag ggtgacgctg gtgaatgcca acgggcaaaa agtggacagt
     2281 ctgcaactga cgacgaatga atttgggtcg tacaatggga cgttccggct gccggagcaa
     2341 caactcaacg gctcgttcag catccaggag gacagcagcc ggggttccac gtccttccag
     2401 gtggaggaat acaagcggcc caaattctat gtggaatacg ggaagcagca gggcagctac
     2461 cgggtgggtg acagtatccg ggtctcgggt ttcgcaaaag ggtattcggg gaatgggatc
     2521 gatggggcga aggtggttta tcacatcacc cggcgggctc atttccgtta tccctggctg
     2581 atctggcggc gcccgatgcc tgccggcgag gcggagatcg ggcatggcga ggtcctgacg
     2641 gatgcacagg ggcaattcaa actccggttt tacgccttgc cggatcaaca gatcagtccc
     2701 gcgacgaagc cggaatacga ttacgagata agcgccgatg tgacggatat caacggggaa
     2761 acgcggaatg gaaaaacgac gcttgtcgcc ggctataccg cgatccggct gactatcgat
     2821 cccgccgggg gcgatcatct gccggtggac agcttgcggg acatcgcggt gagcgctacc
     2881 aatctttccg gggagccggt aggcgccgat gtgggtctgc tgatctatcc gctccaggcc
     2941 ccgcagcggt tgatccgtca gcggctttgg gaagcccccg accgatatat attgccagag
     3001 accgatttcc tggattcctt tccgcatgat gaataccggg aggaaagcaa aaaggagagc
     3061 tgggagaagg ggaaaccgct ttgggaaaag tcctggccga tcggtgactc cggtaaagcc
     3121 agggttgtcc tccccgccgg gctgctgacg acgggttggt atcttatcga ggtagcggga
     3181 accgataaat atggtcagcg ggtgaccgac cggcgctatg taaacctggt cgatggaaaa
     3241 acggggagac cggccaaccc ggaatatgag tggacctccg acggagatgt acgggcggag
     3301 ccgggtggga aggtagctgt caccggcggg tcctcggcgg gtgatgtctt tgtcatccgg
     3361 acagtagagc ggacggaagg gaatgaatat ggtcacttta ccctgaacag ggaagggaag
     3421 aacacggagt ggacgatcac ggaagccgat cgcggcgggt ttagggtgcg ggatgccttt
     3481 gtaaaggaca accggttata tacccattat accacagtgc atgtgccatg gacgaataag
     3541 gagctgagta ttcattatgg gacgtacagg gacaagaccc tgcccggcag tggcgagaag
     3601 tggcagatga cgatcagcgg ttcgaaggga gagcaggtgt cggcggaggt attg
//
LOCUS       DATGFG010001095         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1205260, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001095 DATGFG010000000
VERSION     DATGFG010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205260"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..>2623
                     /locus_tag="VKQ52_20770"
     CDS             290..>2623
                     /locus_tag="VKQ52_20770"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLZ89705.1"
                     /translation="MDTTGMRREIMAVFSRQPQASFAVAFRDLSTGVQFFINEHERFH
                     AASTMKTPVLIETFKLISEHRLSLNDPIPIHIDFTSIADSSRFILDSASDSEKGLYGL
                     AGTKLPLKDLLYKMITESSNLATNLVIELVGAQRVMATMREMGARDIQVLRGVEDTRA
                     FEKGMNNTTTAYDLLLLFDQIATGKAVDPASCEAMIAILKDQHFKESIGGKLPGDVQV
                     ASKSGWITGVCHDSGIVFLPDGRKYVVVLLSRGIGEEGVAQEVLATVSRIIYDHVVGQ
                     SGGVSVVGQVGGLGVAGKAGGLDVTGPVQEPDLRAAIPAIDKMYKAYAERNHYPGMVY
                     GIVADGKLVYSNGIGWSDWQKRIPAGVESDFRIASMTKSFTTVAILQLRDQGKLRLDD
                     PASLYIPELKGQRGTASDAPEITIRNLLTHSAGFPEDNPWGDRQLQATDEELMAMVAQ
                     GLSFSNSPGMYYEYSNLGFTLLGHIIRKVSGLSYEDYITEKVLKPLEMGHTYWEYTKV
                     PADRLAHGYRWLNGEWVEQPMLHDGAYGAMGGLITTMEDFAKYTAWQLAAWPSRDGDE
                     DRVLRRSSRREMQQPWMFNNLNSNFSYNGVDVCPMVSMYAYGIRWSKDCKGRTMVGHT
                     GGLPGFGSNWMILPDYGVGVICFANLTYAGAATINMQVLDSMITLAHLKPRTVPVSAI
                     LAQRQKELVALLPDWSKARESGIFAVNFWQDYFVDSLRKEAEALYAQAGKIVRVGEMH
                     PENRMRGWFLLQGEKADIEVRFTLTPEHSPKIQEYHMR"
BASE COUNT          527 a          564 c          929 g          603 t
ORIGIN      
        1 ggatgaatgg tcagccgctg ccgtccggaa cgtatgtgtg gatggtgcag gggacgacgt
       61 attccaaaaa ggtggtcttc cataagggaa ccatggtttt agtgagataa tgtgtggggg
      121 gcgccggaag ttggcggatg cagggcggac gtttgggcgg ggggagaatg gcgaaaaaag
      181 gttaactttt cggcaaaacc aacgcctgca tgaggaagct gcttttttac ctgtttgttt
      241 atcgatctat tttccgcgag ttgtttttgg cggcgctttg cgatccggtg tggatacgac
      301 cgggatgcgg cgggagatca tggctgtctt ttcccggcag ccacaggcca gctttgcggt
      361 ggccttccgg gacctttcca ccggggtaca attctttatc aacgaacatg agcggtttca
      421 cgcggccagc acgatgaaga cgcccgtgct gatcgagacc tttaagctga tctccgagca
      481 tcggctatcg cttaatgatc ctatcccgat tcatattgac tttaccagta ttgcagacag
      541 cagccgcttt atcctcgact cggcgagcga cagcgagaag gggctttatg gtcttgcggg
      601 aacgaagctg ccgttgaagg acctgctgta caagatgatc acggagagca gtaacctggc
      661 taccaatctc gttatcgagc tggtgggtgc gcagcgtgtc atggccacga tgcgggagat
      721 gggcgccagg gatatccagg tgttgcgggg agtggaggat accagggcct ttgaaaaggg
      781 gatgaacaat acgacgacgg cgtatgacct gctgttgttg ttcgaccaga tcgcgacggg
      841 gaaggcggtg gacccggcgt cctgtgaggc gatgatcgcc attctcaagg accagcattt
      901 caaggaatcg atcgggggga aattgccggg tgatgtgcag gtggccagca agagcggctg
      961 gatcacggga gtctgtcatg attcagggat cgttttcctg ccggatgggc gcaaatatgt
     1021 cgtggtgttg ttgtcgcgcg ggatcgggga ggagggtgtt gcgcaggagg tgctggcgac
     1081 ggtgtcgagg atcatttatg atcatgtggt ggggcagtcc ggtggggtaa gtgttgttgg
     1141 gcaggtggga gggttgggtg tggcggggaa ggccggaggg ttggatgtga ccgggccggt
     1201 gcaggagcct gatctccggg cggcgatccc ggcgatcgac aaaatgtata aagcgtatgc
     1261 cgaacgaaac cattatcccg ggatggtgta tgggattgtt gccgatggga agcttgttta
     1321 cagcaatggt attgggtgga gcgattggca gaagaggatc cccgcgggcg tggagtccga
     1381 tttccggatc gcttccatga ccaagagttt tacgacggtg gcgatcctgc agttgcggga
     1441 ccagggaaag ttgcggctgg atgatccggc ttccctttat atcccggagt tgaagggtca
     1501 gcgggggacg gcttcggatg cgccggagat caccattcgg aacctgctta cgcattccgc
     1561 cggctttccc gaggataatc cctgggggga ccggcagctg caggcgacgg atgaagaact
     1621 gatggccatg gttgcgcagg ggctctcttt ttccaacagc ccggggatgt attatgaata
     1681 cagcaatctt ggatttacgc tgcttgggca tatcatcagg aaggtgagcg ggctttccta
     1741 tgaggactat atcacggaga aggtgttgaa gccgctggag atggggcaca cctactggga
     1801 atacacgaag gttccggcgg accggcttgc gcatggttat cgctggctga acggggaatg
     1861 ggtggagcag ccgatgttgc atgacggggc ttatggggcg atgggcgggc tgatcaccac
     1921 gatggaggac tttgccaaat ataccgcgtg gcagctggcg gcctggcctt cgcgtgatgg
     1981 ggatgaggat cgggtgctga ggcgcagttc gcggcgggag atgcagcagc cctggatgtt
     2041 caacaacctg aattccaatt ttagttataa cggtgtcgat gtctgtccca tggtgtcgat
     2101 gtatgcgtat ggcatccgct ggtcgaagga ttgtaagggg aggacgatgg tcgggcatac
     2161 cggtgggttg ccgggttttg gcagtaactg gatgatcctg cccgattatg gtgtcggggt
     2221 catttgtttt gccaatctta cgtatgcggg tgcggctacg atcaatatgc aggtgttgga
     2281 ctcgatgatc acgctggcgc atctgaagcc ccgaaccgta ccggtgtcgg cgatcctggc
     2341 gcagcggcag aaggagctgg ttgccttgtt acccgactgg agcaaggcaa gggagagcgg
     2401 tatctttgcg gttaatttct ggcaggatta ttttgtggat tcgttgcgta aggaggcgga
     2461 ggcgttgtat gcgcaggcgg gaaagatcgt gcgggtcggg gagatgcatc ccgagaaccg
     2521 gatgcgggga tggtttttgc tgcagggtga gaaggccgat atcgaggtgc ggtttacgtt
     2581 gacgccggag cactcgccga agatacagga gtatcatatg cga
//
LOCUS       DATGFG010001096         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_54253, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001096 DATGFG010000000
VERSION     DATGFG010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54253"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>2163
                     /locus_tag="VKQ52_20775"
     CDS             23..>2163
                     /locus_tag="VKQ52_20775"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLZ89706.1"
                     /translation="MDAAQNVWLASIGAGVLLLRPGSIHSGHIRFERLPPPFEPFLHS
                     EFRAIVGDKEKNIWMAGVNSGLFKYNPYSGVFSHITTDQGLASNTVFCLYCDKEDNLW
                     IGTNSGLQKLVHKDVLFYSSRQGLPADLVLDLLPLPGNSAITCGYSGVGYQHGYGERI
                     RPWKPPLEDEYFLKLTGLKKEYFGLSLRKLISLRAGSTGISARKIFPLPMHFRSMISL
                     GDEGLLLGGDSCILLFRNEKLSTLTRDHVRWISCMALDSSGMLWTGGLDNDIVGYSMQ
                     GSLARPAAQLAFRYKGRATGPQDNVKCMTIDCRQRIVYGMTQSGIYVMARDHGTLKKL
                     FWIGTASGLSNNHVESLLWHDDTTLLAGTGYGLDKIIFPANKDSFYVQNISDFYNFST
                     TIYSIKKDERGNILLGAESGLIKIPSVDIERRALRNLPIVISSIQLLSGKNVTPGAEK
                     TIELPYNNNGLNIYYSSPSFINERTTEYTYQLTGSTQHQWSQPSFSNHVTLLNLSPGN
                     YHFAVRPVNIYGQASSQTAGLEIVIRPPVWQRWWFPFLLLGLVAALAFLVVRRRISAI
                     RRESAMKNKIAETEMMALRAQMNPHFIFNCMNIIDGLITNNRKAEALNFLQKFSKLIR
                     LVLENSQYQEVPLNQDLQALRLYIELEVVRSNNHFSYIFDIDRELQEQNYKIPPLLLQ
                     PYIENAIVHGLRNKESGDGILRVDIKKAGDS"
BASE COUNT          545 a          565 c          553 g          500 t
ORIGIN      
        1 gttatttgta cgacatattt acgtggatgc ggcgcaaaac gtctggcttg cgtccattgg
       61 agcaggggta ttgcttttgc gccccggatc gatccattcc ggccatatca ggttcgaaag
      121 actgcctccc ccattcgagc cttttcttca ttccgagttc agggccattg tcggggacaa
      181 ggaaaagaac atctggatgg cgggtgtcaa tagcgggttg tttaagtata acccgtacag
      241 cggggtattc tcccatataa caaccgatca gggtcttgcc agtaacaccg tcttttgcct
      301 gtattgcgat aaggaggaca atctctggat cggcacaaac agcgggctac agaaactggt
      361 gcacaaagat gtccttttct attcctccag gcaggggtta ccggcagacc tggtgcttga
      421 tctgctgcca ttgccgggca atagcgccat tacatgcggt tattcaggcg ttgggtatca
      481 gcatggttat ggcgaaagga tccgaccgtg gaagccgccc ctggaagacg aatactttct
      541 gaaacttacc ggcctgaaga aggaatattt cgggctttca ctcagaaaac tgatttccct
      601 gcgggcaggc agtaccggca tttcagcccg gaaaatattt cccctgccca tgcatttcag
      661 gagcatgatc tccctcgggg acgaagggtt attgctgggc ggagacagct gtatcctctt
      721 gttccggaat gaaaaactaa gcacactgac gagggatcac gttcgttgga tcagctgcat
      781 ggccctggat tcgtccggca tgctgtggac cggaggcctg gacaatgata tcgttggata
      841 ttcgatgcag ggtagcttag cccggccggc cgcacagctc gccttccgct acaagggcag
      901 ggccaccggc ccacaagaca atgtaaaatg catgaccatc gactgccgcc agagaatagt
      961 ctatggcatg acgcaaagcg ggatctatgt aatggcccgg gatcatggga cgttgaaaaa
     1021 actattttgg atcggtacgg cttcgggatt gagcaataac catgtcgaat cgctcctgtg
     1081 gcatgacgac actaccttgc tggccggcac cggttatgga ctggataaga tcatcttccc
     1141 ggcaaataaa gacagctttt atgttcaaaa tatcagcgat ttctacaatt tctccaccac
     1201 gatatacagc atcaagaagg acgaacgggg caatatcctg ttaggcgcag aatcgggcct
     1261 tattaaaata ccttcggtcg atatcgaacg ccgtgccctc aggaacctgc ccatcgtcat
     1321 ttcatccatt cagctgctaa gcggaaagaa cgtgactccc ggcgccgaaa agaccatcga
     1381 gctgccctat aacaacaacg gattgaacat ctattactcg tcgccgtcct ttatcaatga
     1441 acggaccacc gaatatactt accagctgac cggcagcacc cagcatcagt ggagtcagcc
     1501 ttccttttcc aaccatgtca cattgctcaa cctgtccccg ggaaattatc actttgcggt
     1561 aaggcccgta aacatctatg gccaggcatc aagccaaacc gccgggttgg aaattgttat
     1621 ccgccctcct gtatggcaac ggtggtggtt tccattcctg ctgttgggat tggttgccgc
     1681 gttggctttt ctggtcgtaa gaaggcggat cagcgccatc cggcgggaat ccgcgatgaa
     1741 gaacaagatc gccgaaacgg aaatgatggc gctaagggct cagatgaacc cgcatttcat
     1801 cttcaattgc atgaatatca tcgacggcct catcaccaat aatagaaagg cggaggccct
     1861 gaacttcctc cagaagttct ctaagcttat ccggctggtg ctggagaact cccagtacca
     1921 ggaagttccg ttgaaccagg acctgcaggc cctccgattg tatatcgagc tggaagtcgt
     1981 acgcagcaat aaccatttca gctatatatt cgatattgac cgcgagctac aggaacagaa
     2041 ttataagatc ccacccctcc tgctccagcc gtatatcgag aacgccatcg ttcatggact
     2101 gaggaacaag gaaagcgggg atgggatact gcgcgtcgac atcaaaaaag caggggactc
     2161 cat
//
LOCUS       DATGFG010001097         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_178242, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001097 DATGFG010000000
VERSION     DATGFG010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178242"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VKQ52_20780"
     CDS             <1..953
                     /locus_tag="VKQ52_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89707.1"
                     /translation="SDERVLWMMGDSYINHYDTLAGTTGCLFQVRNCALLQPLNRWGW
                     TETSTLIGHGPGVPSLFRNDTSSHDLLWPTGGYQYGDTVYVYNTSQKDTAGGLGFTRG
                     GNDVLAKMLLPGLQVAGYDPLRSFGTASFGLGFDADEPGDYVYTWGIQPAYINCHIVV
                     ARFPRNNPRAPWAFWNGKAWDTAVAHMASIATGASNGSYVAKVRNKYVLVSTEFSVSC
                     DAGTRIYVATSDRITGAFTDNRVLYTIPDNVLGHTPFFYGPVLHPEYINSKNEILITY
                     DINGYSNCEPNCIGNGFNPDYYRPRGLRVPLGLIISSKIY"
     gene            1005..2336
                     /locus_tag="VKQ52_20785"
     CDS             1005..2336
                     /locus_tag="VKQ52_20785"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLZ89708.1"
                     /translation="MRKVESPPYVPADWDFGELLDELEAKQKYVEAREIDDSSYPYWE
                     KWKYLAREWRLDPRRVWAAIKKRRGISRRTVNFDIGGRFKPQINTPSIMQQQLHELDL
                     SLGGNLDASDIIPSEEKQRYLISSLMEEAIASSQLEGAATTRKLAKQMLESNRKPRTS
                     GEQMIVNNYSAMQWIVQNKKTPITEESILHLHRLITNDTLANKDEEGAFRTTDDVRIV
                     DVQTGEVLHTPPKPEGIPQLIQAFCLFMNDEQKEDFFLHPISKAIIVHFLIGYIHPFA
                     DGNGRTARALFYWYLIRKGYWLIEYMSVSRIILGSKAQYSRAYQHTEKDENDLTYFVL
                     YNLHCIQRSLEELKKYIERKNREKKSILKLLRSTDYNDRQIVLIEEILNEKKAVFTVT
                     EVQHKFGVANQTARTDLEYLVSKGILEARKSGNKVRFLLAEDGEKKIMGKG"
     gene            complement(2353..2868)
                     /locus_tag="VKQ52_20790"
     CDS             complement(2353..2868)
                     /locus_tag="VKQ52_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89709.1"
                     /translation="MKKKMICILLASLWVMAANAQKETKKFSMGFGLEAGVPSGNLGN
                     LYTTAIGLTIRASWHAGPGFATLTSGLLGYVPKKIIGVPSKAGLQIPVRAGYKYIIQH
                     HLFLMGEVGYSDFNTYYGYKGSLVSTSSGALLVAPSIGFQANAFELGIRYDMFTNSTG
                     SVAAVRLGFNF"
     gene            3017..>4296
                     /locus_tag="VKQ52_20795"
     CDS             3017..>4296
                     /locus_tag="VKQ52_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89710.1"
                     /translation="MVIILWLGTCAQQGVPIYAKRCPVKAINYEQGLMDNSITAVITD
                     TEGFTWVSTSSGLQRYNGYSLQQVMPVVAGDTIPIDYPVFFIKGKDKALLIGYREGLL
                     KYSTETNSFQKIIFRAAPEGSHLHSLMPLKETTEGIWCFDETKGVVLYRHNGAGLERT
                     PSIINATIINLIRTEEYFITRRLVACNENYIFIRLSPNRILEINAGTHQSKRLDYPDS
                     LITGLECNGDKLFVSSSESISVQEIGTGVITKRFPIRWLSNDPKVTRSSVGLSSDGHL
                     LVSEEGRLFEFDTSGDCRKEIISLNRDPLLKTGYIQIVYEDPFRRIWLLTNSDIKRIE
                     NVETPFSYLTYPNARSHFIRSLYYDSAKKTLLAGEFLGPFELYDSAGNPMWQEPLTDS
                     RCASPVAIDKLGDDRYLVITAGKGWFLLNSATRR"
BASE COUNT         1078 a         1064 c         1163 g          991 t
ORIGIN      
        1 tgtccgatga acgggtgctt tggatgatgg gcgacagcta tatcaatcat tacgatacgc
       61 tggcgggtac gacgggatgt cttttccagg tgcgcaattg tgcgctgctg cagcccttga
      121 atcgctgggg ctggaccgag acgtccacgc tgatcgggca cgggccgggt gtgcccagtt
      181 tgttcaggaa cgataccagt agtcacgatc tgctatggcc tacgggcggg tatcaatacg
      241 gcgatacggt ctatgtatac aacaccagtc agaaagatac ggccggcggt ttggggttca
      301 cccgtggcgg gaacgacgtg ctggcgaaaa tgctgctacc cggattgcag gtggccggct
      361 atgacccgtt gcgcagcttt ggaaccgcgt cgtttggttt gggattcgac gccgatgaac
      421 cgggcgacta cgtctatacc tggggaatac agccggccta catcaactgt catatcgtgg
      481 tggcgcgttt tccccggaac aacccgcggg cgccatgggc tttctggaat gggaaagcct
      541 gggatacggc agtcgctcac atggcaagca ttgccaccgg cgcctccaat gggtcgtatg
      601 tggcgaaggt gcggaataaa tatgttttgg taagcacgga attcagtgta tcctgcgatg
      661 ccggaacgcg gatctatgtg gcgaccagtg acaggattac cggtgcgttc accgacaaca
      721 gagttcttta cacgataccc gacaatgtat tggggcatac gcctttcttt tatggtcctg
      781 tccttcatcc cgaatatatc aattcgaaaa atgagatcct gattacgtat gatatcaatg
      841 ggtatagcaa ttgtgagccg aattgtatcg ggaatgggtt caacccggac tattaccggc
      901 cgcgcggatt gcgggttccg ctggggttga taatttcttc taaaatctat taaattagga
      961 taaaaataag aaaaaactcc taattttatg aaaactagga gtaaatgaga aaggttgaat
     1021 ccccacccta tgtccctgcc gattgggatt ttggggagct tttggatgaa ttggaggcca
     1081 aacagaaata cgtggaagcc agggagatag acgactcgtc gtatccatat tgggaaaaat
     1141 ggaagtatct ggcccgtgaa tggcgactcg atccccggcg ggtttgggca gcgataaaga
     1201 aaagaagggg tatttcccgc cgtacggtga attttgatat tggcggccgg ttcaagcccc
     1261 aaatcaacac gccgtcgatt atgcagcagc aattgcatga gttggattta agccttggcg
     1321 gcaacctgga tgccagtgac atcatcccat ccgaggagaa acaacggtac ctgatcagtt
     1381 cgctgatgga agaggccatc gcatccagcc aactggaggg cgcagcgacg acgcggaaat
     1441 tggccaagca gatgctcgaa agtaatcgca agccgcggac ttccggcgaa caaatgattg
     1501 taaacaatta ttccgccatg caatggattg tgcagaacaa gaagacgccc attaccgaag
     1561 aaagtattct tcatctccat cgccttatca ccaacgacac tttggcgaac aaggacgaag
     1621 agggcgcttt tcggaccact gacgatgtac ggatcgtcga tgtgcagacc ggcgaggtat
     1681 tgcatacacc accgaaaccg gaaggcatcc cacaactgat acaggcgttt tgcctcttta
     1741 tgaacgatga gcaaaaagag gatttctttt tgcacccgat ctcaaaggca atcatcgttc
     1801 atttcctgat cggatatatc catccgtttg ccgacggcaa cggccggacc gcacgtgcat
     1861 tgttctattg gtacctgatc cggaagggct attggttgat cgaatatatg tccgtatccc
     1921 gaatcatcct gggatccaag gcacagtatt cgcgtgctta ccagcataca gaaaaggacg
     1981 agaacgatct cacctatttc gttctctata atctccactg tattcaacgt tcgctcgaag
     2041 aactaaaaaa atacatcgag cggaagaacc gggagaagaa aagtatcttg aaattgctca
     2101 ggagtaccga ctataatgat cgtcagatcg tgctcatcga ggagatcctc aacgagaaaa
     2161 aggcagtctt taccgtcacg gaggtgcagc ataagttcgg agtggccaat caaaccgccc
     2221 ggacggattt ggaatatctg gtatcgaaag gcattctgga agcccgcaag agtggcaata
     2281 aggtgcggtt cctgctggct gaggacggcg agaagaagat catgggaaaa ggatagcgcc
     2341 ggtttggggc tactagaaat tgaagccgag cctgacggcc gctacgctgc cggtgctatt
     2401 cgtgaacata tcataccgga tgccgagctc gaacgcattc gcctggaagc cgatggaagg
     2461 cgctacgaga agggctccgc tggaggtaga gacgagactg cctttgtagc cgtaataggt
     2521 gttgaaatcc gaatagccta cttcacccat gaggaacagg tggtgttgga taatatattt
     2581 gtagcctgcg cgtacgggga tctgcagccc ggcctttgaa ggcacgccta taattttctt
     2641 cggaacatag ccgagtaacc cgcttgtcag cgtggcaaag cccggcccgg cgtgccagga
     2701 agcgcggatc gtcaggccga tggcggtggt atacaaatta cccagattcc ccgatggcac
     2761 gcccgcttca aggccgaatc ccatactgaa tttctttgtt tctttctgag cattggcagc
     2821 cattacccat agcgatgcca gtaaaatgca gatcattttc tttttcatac acttgctttt
     2881 agttggttgt gaaatatgta tcgggaagga ctatgatgca agactaccac ttcgcgcggc
     2941 ggaaatcaat aacacatatg tgttatttta ttttgtaact tgaagaatgg ccaaaacttg
     3001 ctgtatacta tccccgatgg ttatcatcct ttggctgggg acatgtgcac agcagggggt
     3061 cccgatatat gccaagcgat gtcccgtgaa ggcgatcaac tatgagcagg ggctcatgga
     3121 caattccatc accgccgtaa taaccgacac cgaagggttc acctgggttt ccacgtccag
     3181 cggtcttcag cgatataacg ggtatagcct gcaacaggtg atgccggtgg tggccggaga
     3241 cacgatcccc atcgactacc cggtattctt tataaaggga aaggataaag cgctgctgat
     3301 cggttatcgg gagggattgt tgaaatactc caccgagacg aattctttcc aaaagattat
     3361 tttcagggcc gccccggagg gcagccacct gcactcgttg atgccgctga aggaaacgac
     3421 ggaagggatc tggtgtttcg acgagacgaa gggagttgtc ctctatcggc ataacggcgc
     3481 cggtctcgaa cggacccctt ccatcatcaa cgcgaccatc atcaacctca tccggaccga
     3541 agaatatttt atcacccgca ggcttgtcgc ctgtaatgag aactatatct ttattcgcct
     3601 gtccccgaac aggatactgg agatcaatgc gggcactcat caatcgaaac ggctcgatta
     3661 tcccgactcg ctcatcaccg gtctcgaatg caatggcgat aagctcttcg tatcctcctc
     3721 cgagagcatc tccgttcagg agatcggcac cggcgtcata acaaagcgat ttcccatcag
     3781 gtggctcagc aatgacccta aagtgacgcg gagcagcgtg ggattatctt cggacggtca
     3841 cctcctggtc agcgaagagg gccggctttt tgagttcgat acttccggcg actgccgtaa
     3901 agagatcatt tcgctgaaca gggacccttt gctaaaaacc ggctatatcc agatcgttta
     3961 tgaagaccct ttccgccgta tctggctgct gacgaacagc gatatcaagc ggatcgagaa
     4021 tgtggagacg cccttttcct acctgaccta tccgaatgcg cggagtcatt ttatccggag
     4081 cctgtattat gacagcgcga aaaaaacgct cctggcgggg gagttcctcg gaccattcga
     4141 gctctacgat tccgccggca acccgatgtg gcaggagcca ttgaccgata gccgatgtgc
     4201 cagccccgtg gccatcgata agcttgggga tgatcgctac ctggtcatca ccgcgggtaa
     4261 aggctggttt ctgctgaatt cggccacccg ccggtt
//
LOCUS       DATGFG010001098         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_178352, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001098 DATGFG010000000
VERSION     DATGFG010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178352"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VKQ52_20800"
     CDS             <1..702
                     /locus_tag="VKQ52_20800"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ89711.1"
                     /translation="LGVPNKPTPGDTRKTKLPENFVIGPLTKELHLYSDGITGFIAVR
                     FHPWGFSAFSAQPATALVDAILPAARALYADIRPLEQQLLNNPSREKKLQVLTAWFTN
                     TPPRQSPDLPAIEAIATALRTQHGTQKITDLARQFNINPRKLERHFLQHIGLPAKRFA
                     RILRFNHAKNLIQKNPDIPLAELAYETGYADQAHFSKNFRQLFDCSPAQFKTRIKKFL
                     ADTSGLDKDVVFIQD"
     gene            complement(712..1605)
                     /locus_tag="VKQ52_20805"
     CDS             complement(712..1605)
                     /locus_tag="VKQ52_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89712.1"
                     /translation="MKYLYSLGFVWLCAGCSLLSSKKKVFDYCYVNSKGLYAVAYGKE
                     PTRVYVNGTDPQLSPDGRKIAYTDVGARDHQRRIGVFDLEAGKVTILDTSCQNCYGPV
                     WSPDGNYIVYNAFTGKDWGIKYVDKDNQHPVVLATPADSLLVFSSPTWSADSRKIVVG
                     ASGRTGPQAVYIYGLDGRVLQSISFEQLDTSLAFSSASTFVLNRGEDKLVYWASVSQE
                     SKDGESPTAVFVHDLAAGRSVRISPKGYVCWQPVLKGDTVFCNGKHSGSGKENIYRMD
                     LDGGHFALAYKNRADVSFASR"
     gene            complement(1671..>2990)
                     /locus_tag="VKQ52_20810"
     CDS             complement(1671..>2990)
                     /locus_tag="VKQ52_20810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ89713.1"
                     /translation="ALVEPVSEAVNHRHLVMNILVTFVLVCVFFYFVLRLLDFKEKRN
                     KRDIAALKLVEVELLQAKAKAEKAAATKARFMSNMSHELRTPLNAIIGTMHLLIQDQE
                     SLHDSDHFNVLKDSSEHMLQLVNAVLDFSKLDEGKLEFVQESFDLAATLQQATDSFIP
                     VVQQKNIELRLETDVLPAGKRVVGDEMRLKQVLLNLLSNAVKFTESGSVTLRSQIRNT
                     TDSGVEIRFAVEDTGIGIPTEKLPIIFESFTQADAETTRKYGGSGLGLSICRELVKKM
                     GSELKVSSEPGKGSVFFFDVLLPFQQDVMIVPKEKLRGLQKLTGIRILLVEDNAVNMR
                     IARRFLHSWGASINTAENGQVAWDLFQQEPYDLLLVDLEMPLMDGKALLTQVRNVNKD
                     VPAIAFTAAVYENMYDDLQRHGFNGYLHKPFRPDEMHRKILRHIVKK"
BASE COUNT          715 a         1083 c          599 g          593 t
ORIGIN      
        1 ctcggcgtcc ccaacaaacc cacccccggc gacacccgca aaaccaaact ccccgaaaac
       61 ttcgtcatcg gccccctcac aaaagagctg cacctctatt ccgacggcat caccggcttc
      121 atcgccgtcc gcttccaccc atggggcttc tcggccttct cggcccaacc ggcaacagcc
      181 ctcgtcgacg cgatcctccc cgccgccagg gccctctacg cggacatccg gccactcgaa
      241 caacaactgc tcaataaccc gtcccgcgaa aaaaaactcc aggtcctcac cgcctggttc
      301 actaacacgc cgccaagaca gtcgccggac cttccggcca tcgaggccat cgccacggcg
      361 ctccggacac agcacggcac ccaaaagatc accgacctcg cccggcagtt caacatcaat
      421 ccgcgcaagc tcgaacggca tttcctccag cacatcggcc tgcccgcaaa acgcttcgcc
      481 cgcatcctcc gcttcaacca cgccaaaaac ctcatccaaa agaaccccga catcccgctc
      541 gccgaactgg catacgaaac cggctacgcc gaccaggcgc atttcagcaa gaacttccgg
      601 cagctcttcg actgctcacc ggcccaattc aaaacgcgga taaaaaaatt cctggcggat
      661 accagcggcc tcgacaagga tgtcgttttt atacaagact gaccccatcg cctaacgcga
      721 cgcaaaactc acgtccgccc tattcttata cgcgagcgca aaatgccccc catccagatc
      781 catccggtaa atattctcct tcccggaccc actatgcttc ccattgcaaa aaaccgtatc
      841 ccccttcaac accggctgcc agcacacata ccccttcggc gagatccgca cactcctgcc
      901 cgccgccagg tcatgcacaa acaccgcagt cggcgactcc ccatccttcg actcctggct
      961 caccgacgcc caatacacca gcttatcctc cccccggttg agtacaaagg tcgaagcact
     1021 cgaaaacgca aggctcgtat ccaactgctc gaaggagata ctctgcagca cccgcccatc
     1081 cagcccgtag atatacaccg cctgcggacc cgtcctgccg gaagctccca ccacgatctt
     1141 ccggctgtcg gcgctccacg tgggggaaga aaaaacaagc aggctatcgg caggcgtagc
     1201 caacaccacc ggatgctggt tgtctttgtc gacatatttg atgccccaat ccttgcctgt
     1261 aaaggcattg tagacaatat aattgccgtc gggcgaccaa accggcccat aacagttctg
     1321 acaggaagtg tccagtatcg tcaccttccc cgcttcaaga tcgaacacgc cgatccgccg
     1381 ctgatgatcc cgcgccccca catccgtata ggcgatcttc cggccatcag gcgaaagctg
     1441 cgggtccgtg ccgttcacat acacccgcgt gggctccttg ccataggcaa cggcataaag
     1501 ccccttgctg ttgacataac aataatcgaa aaccttcttt ttactggaaa gcaaactaca
     1561 tcccgcgcag agccatacga accccaaaga gtacagatat ttcataaata cggattttat
     1621 taacaatatg cgccgccctt ctcctttgtc tgccaatggc gggcggcgcc tcatttcttg
     1681 actatatgcc gcaatatctt ccggtgcatc tcatcgggcc tgaacggctt atgcaaatac
     1741 ccattgaacc catgccgttg cagatcatca tacatattct catacaccgc cgccgtaaac
     1801 gcgatcgccg gcacatcctt attcacattc ctcacctgcg tcagcaacgc cttcccatcc
     1861 atcaacggca tctccaggtc caccagcaac agatcatacg gctcctgctg aaaaagatcc
     1921 caggccacct gcccgttctc cgccgtattg atactcgccc cccagctgtg caggaatcgc
     1981 cgcgcgatcc gcatattcac cgcattatct tcaaccaaaa ggatacgtat ccccgttagc
     2041 ttctgcaatc cccgcaactt ctccttcggt acgatcatca catcctgctg aaagggtaac
     2101 agcacatcga agaaaaagac actccccttt cccggttcgc tgctcacctt caactcactg
     2161 cccattttct tcaccaattc cctgcaaata ctgagcccaa gtcccgaccc cccgtatttc
     2221 cgcgtagtct ccgcgtcagc ctgcgtaaaa ctctcaaaga tgatcggtaa tttctctgtg
     2281 gggatcccga tacccgtgtc ctccaccgca aaccggatct ccactccgct gtccgtggta
     2341 ttcctgatct gcgaccgcag ggtcaccgac cccgactcgg taaacttcac cgcattcgaa
     2401 agcaggttga gcaacacctg cttcagccgc atctcatccc ccaccacacg cttccctgcg
     2461 ggaagcacat ccgtctcgag ccggagctcg atattcttct gctgcaccac cgggataaac
     2521 gaatccgtcg cctgctgcaa ggtcgcggca agatcgaacg actcctgcac aaactccagc
     2581 ttcccctcgt ccagcttgct gaaatcaagc accgcattca ccaactgtaa catatgctcc
     2641 gaagaatctt tcaatacgtt gaaatgatcg ctgtcatgca acgactcctg gtcctgtatc
     2701 agcaggtgca tcgtgccgat aatggcattc aacggcgtcc gcagctcgtg actcatattg
     2761 ctcatgaacc tcgccttggt cgccgccgcc ttctccgcct tggccttcgc ctgcagcaac
     2821 tccacctcca ccagcttcag cgcggcaatg tcgcgtttgt ttcgcttctc tttaaaatcg
     2881 agcagccgca acacaaaata gaagaacaca cacaccagca caaacgtgac aaggatattc
     2941 atcacgagat gccggtgatt cactgcctcg gacaccggct ccaccagcgc
//
LOCUS       DATGFG010001099         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1012511, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001099 DATGFG010000000
VERSION     DATGFG010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012511"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VKQ52_20815"
     CDS             complement(<1..185)
                     /locus_tag="VKQ52_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89714.1"
                     /translation="MNYIDIRSISELHDFFRYEKPVHPQISVVDLAKVDRSHREPGAS
                     YRIDLYSVACKKIEGFFG"
     gene            complement(268..1041)
                     /locus_tag="VKQ52_20820"
     CDS             complement(268..1041)
                     /locus_tag="VKQ52_20820"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ89715.1"
                     /translation="MSTSTTTQQSRIALVTGGSRGIGKSIAINAAKRGIGIILTYNSH
                     PGQGEAVAKEINNSGGKAVALKLDTANTSSFDDFVSQVAHVLKREWRRDSVDYLVNNA
                     GIAQRTLIKDTTEEIFDQLVDVNFKGVFFLTQKLMPLMADDGQIINISSGLARFAFPG
                     VAVYGALKSALEGLTRYFAKEYAERRIRVNTVAPGAIDTEFGGGKGDESHRQQIANTT
                     ALGRLGEADDVGLFVASLLSDDSRFVNAQRIEVGGGIYI"
     gene            complement(1119..1988)
                     /locus_tag="VKQ52_20825"
     CDS             complement(1119..1988)
                     /locus_tag="VKQ52_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89716.1"
                     /translation="MRYWLTIVFATITTFGLQAQPAARVLHFSSPHTCFPDTARDHGH
                     LDGDNILQPRAGHYDDSSVLLVVPPAFRAGKTIDLVVWFHGWHNNIDTALRFYGLADQ
                     FAASGRNAVLVLPEAARNAADSYGGKMGQQGMFQLLVGDVIDELKRKGIAPQDAIPGH
                     IVLAGHSGAYGVIADILASGRQPVDEVFLFDALYSRVPIFTHWAEEKGHHFVHWFTNT
                     GYGPDKMSDTMMLRLQQAQVPYGLCEERSVTGDILNDNRILFIHSPRQHNVIINNPDD
                     FALLLKNSFYLHQ"
     gene            2103..2426
                     /locus_tag="VKQ52_20830"
     CDS             2103..2426
                     /locus_tag="VKQ52_20830"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLZ89717.1"
                     /translation="MIIRRDVFQAIADPTRRAILLLVAAHAMTAGAIASNFDTARPTV
                     SKHLQILTECELLEQKQNGREIYYYLNAKKMKEVADFIEPFRAMWDDRFNKLEAIMKN
                     YKPKK"
     gene            2432..2899
                     /locus_tag="VKQ52_20835"
     CDS             2432..2899
                     /locus_tag="VKQ52_20835"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLZ89718.1"
                     /translation="MEQKTKVHAEDGKHDMTITREFDLPVELLFKAHADAEILEQWMT
                     SKVVVLESKKNGSYLLEKTDDKGNVVFQARGVIHEFNPNRKIIRTFEMGNTPFGVQLE
                     IYEFEKLTDETSKLRMHVIYESVEQRDQVLQLPFTQGLNWAHNRIEEILSKVK"
     gene            2906..3298
                     /locus_tag="VKQ52_20840"
     CDS             2906..3298
                     /locus_tag="VKQ52_20840"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLZ89719.1"
                     /translation="MEKRNKIIYWIATIWLSLGMFSTGLVQLFKARSGPSGADSLAHL
                     GYPGYFLLLIGVWKILGVVAVLVPHFPRLKEWAYAGFFFAMSGAVISHLALGDHFAET
                     FPSILLLVLTSVSWYFRPASRKLILTVQ"
     gene            3306..3887
                     /locus_tag="VKQ52_20845"
     CDS             3306..3887
                     /locus_tag="VKQ52_20845"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HLZ89720.1"
                     /translation="MNPRVDFFFDKASKWQEEYEKLRMLLLDCGLNEELKWGCPCYTF
                     QKSNIVLIHGFKDYCALLFFKGALLRDPDGILIQQTENVQVARQIRFTSVKEIGKMAT
                     ILKAYVYEAIEVDKAGVQVKLKKTAEFSVPEEFKRKLDKTPALKKAFNALTPGRQRGY
                     LLHFSAPKLAKTRESRIEKCMPLILKGKGLDDL"
BASE COUNT          971 a          944 c         1053 g          933 t
ORIGIN      
        1 ccgaagaatc cctcgatctt tttacaggcg acggaataca gatcgatccg gtaggaggct
       61 cccggctccc ggtgagagcg atcgaccttt gccagatcga cgacagagat ctgggggtgc
      121 accggctttt cataccgaaa aaagtcatgc aattcagaaa tcgatctgat gtctatatag
      181 ttcatgaatg gtattatttg tcggtctatg gaaatagtcc atcgatatcg tccatgcaat
      241 ctaattctct ttccggagtt gccgaaatca gatataaatc cctccgccta cctcgatgcg
      301 ttgggcattt acgaagcgac tgtcgtccga aaggagggat gccacaaaaa gaccgacgtc
      361 gtcggcctcg ccgagccggc ccagggccgt cgtattggca atttgctgac ggtgactctc
      421 gtcgcctttt ccgccgccaa actcggtgtc gatggctccc ggcgcgacgg tattgacgcg
      481 aatcctccgc tcagcatatt cttttgcgaa ataccttgtc agtccttcca gcgcggattt
      541 gagtgcccca tagacggcga ccccgggaaa tgcgaagcgg gcaagcccgg aggagatgtt
      601 aatgatctgg ccgtcatctg ccattaacgg catcagcttt tgggtgagaa aaaatacgcc
      661 tttgaagttg acatccacga gttggtcgaa gatctcttcg gtggtgtctt ttatcagcgt
      721 tcgctgggcg atgccggcat tattgacaag ataatcgacg ctatcccggc gccattctct
      781 tttcaggaca tgggcgacct ggctgacgaa atcatcgaaa gaggaggtat ttgccgtgtc
      841 gagctttaaa gcgacggcct tgccaccgct gttattgatc tcctttgcca cggcttctcc
      901 ctggccgggg tgactattgt aggtgaggat gatgcctatc ccgcgttttg ccgcattgat
      961 cgcgatgctt ttcccgatac cccggctccc cccggtgacc agggcgatcc ttgattgttg
     1021 cgttgttgtg cttgtgctca taaaatgaaa tttatgacac aaagttccta catgctatcg
     1081 cagtgggctt atacatttaa ggccgggact tatacttttc actgatggag gtaaaagcta
     1141 ttttttagca acaaggcgaa atcatccgga ttgttgatga tcacattatg ttgtcgtggg
     1201 ctatggatga atagtatccg gttgtcgttc aggatgtcac cggtgaccga tctttcctcg
     1261 cataatccat atggcacctg tgcctgctgc agccgaagca tcatcgtatc gctcatcttg
     1321 tccggtccat agccggtatt ggtgaaccaa tgcacaaaat gatggccttt ttcctccgcc
     1381 caatgggtaa agatgggcac acggctatag agcgcatcga ataagaacac ctcgtcaacg
     1441 ggctggcgcc cggatgccag gatatcggcg atcaccccgt atgcaccgct atgtcctgcg
     1501 agcacaatat gtccggggat cgcatcctgc ggggcgatgc cctttcgttt taattcgtcg
     1561 atcacatcgc ctaccagcaa ttggaacatc ccctgttggc ccatttttcc tccatagcta
     1621 tcggccgcgt tcctggccgc ctccggcagc acgagcacgg cgttccgccc ggaagcagcg
     1681 aactggtccg ccagtccata aaatctcaac gccgtatcga tattattgtg ccagccatgg
     1741 aaccagacca ccaggtcgat ggttttcccc gcccggaatg ccggcgggac caccaatagt
     1801 accgagctat cgtcataatg tcccgcccgg ggttggagga tgttgtcgcc atccaggtgt
     1861 ccatggtccc ttgccgtatc gggaaaacaa gtatgcgggg aggaaaaatg cagtacccgg
     1921 gcggcgggct gtgcctgaag cccaaaggtg gtgatagtcg caaaaactat tgttaaccaa
     1981 tatctcatag gctatgaata tacactttgc cggggggaaa gcgggccggt gagaaaaaaa
     2041 tccgaaaaaa aatttgcgta gtcagatagc tacacatata tttgtagcca aatagctaca
     2101 taatgatcat cagaagagac gtgttccagg ccatcgccga cccgacaaga agggcgatcc
     2161 tgttgttggt ggcggcgcac gccatgacgg caggggctat agcgtcgaat ttcgacacgg
     2221 caaggccgac ggtttcaaag catctgcaga tacttaccga gtgtgaattg cttgaacaaa
     2281 agcagaacgg cagggaaatc tattattatc taaatgccaa aaaaatgaaa gaggtagccg
     2341 actttatcga gccgttccgc gcgatgtggg atgaccgatt taacaaactg gaagcgataa
     2401 tgaagaatta caaacctaaa aaataaatac catggaacag aaaacaaagg tccatgccga
     2461 ggacggcaag catgacatga cgatcacgag ggaattcgat ctgccggtcg aactgctttt
     2521 caaggcgcat gcggatgcgg agatcctcga acagtggatg acatcaaaag tggtggtgct
     2581 ggaaagtaag aagaacggca gttacctgct tgaaaaaacg gatgataagg gcaatgtggt
     2641 gttccaggcc cgtggggtga tccatgagtt caacccgaac cggaagatca tccggacgtt
     2701 cgaaatgggg aatacaccct ttggcgttca gctcgagatc tatgagtttg aaaagctgac
     2761 ggatgagacc agcaagctcc ggatgcatgt gatctatgaa tcggtggagc aacgggacca
     2821 ggtgttgcag ttgcccttta cccagggact caactgggct cataaccgaa tcgaggaaat
     2881 tttaagtaaa gtaaaataaa ctaccatgga aaagagaaat aagatcatct attggatcgc
     2941 caccatctgg ctttccttag gaatgttttc gaccgggtta gtacagctat tcaaggcgag
     3001 atccgggcca tcgggggcag acagcctggc acatttaggg tatcccggct attttctcct
     3061 gctgataggt gtctggaaga tccttggggt ggtggccgtg cttgtccccc acttcccgcg
     3121 gctgaaggaa tgggcttatg cgggcttctt ctttgccatg tcaggggcgg tgatctccca
     3181 tctcgcgttg ggcgatcatt ttgcagaaac attcccttcg atactgctcc tggtgctgac
     3241 gtcggtgtcg tggtatttcc ggcctgcgag tagaaaacta atcctaactg ttcaataatc
     3301 atcatatgaa tcccagggtt gatttctttt ttgataaggc cagcaagtgg caggaagaat
     3361 atgagaaatt gagaatgctc ctgcttgact gtgggctgaa cgaagaattg aagtggggtt
     3421 gtccctgtta tacgttccag aaaagcaata tcgttctcat acatggattt aaagattact
     3481 gtgcgcttct cttcttcaag ggcgccttgt tacgggaccc cgacggtatc ctgatccaac
     3541 aaacggagaa tgtgcaggtg gcccgccaga tccggttcac cagtgtcaag gaaataggca
     3601 agatggcgac catcctgaaa gcgtatgttt atgaagcgat cgaggtggac aaggcggggg
     3661 tgcaggtgaa gctaaagaag actgccgaat tctccgtgcc ggaggaattt aagcggaaac
     3721 tggataagac gccggccttg aaaaaggcat ttaatgccct gacgccgggg cggcaaagag
     3781 gttacctgct gcatttttcc gctcccaaac tggccaagac ccgggagtcg aggattgaaa
     3841 aatgcatgcc gctaatcctt aagggaaagg ggttggatga tctgtagggg cggcagttgt
     3901 t
//
LOCUS       DATGFG010001100         7258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1695764, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001100 DATGFG010000000
VERSION     DATGFG010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695764"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..2730
                     /locus_tag="VKQ52_20850"
     CDS             304..2730
                     /locus_tag="VKQ52_20850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89721.1"
                     /translation="MFTNYLKTAFRNLLKNKGFTALNVLGLTLGVATCLLIVFYVLDE
                     VSYDRYNEKADRIYRINNMIKFGGNENTYAGSPAPAAAALKNDFPEIGQVVRFIKSGG
                     IQVKKGGQYIQEDHAIFADSSLFAVFTLSMIDGKPATALRDPHSAVITEQTAKKYFNR
                     TDVVGQTLLIGDTDAYKITGVIRDIPAESHFRCNFFFSLSTLAYSREDAWLDNNLITY
                     VLLEKGADPARLDAKFPAFIRKYMGPQLQGALHLTFDDFEHGGNYYRFKLFPLTSIHL
                     RSNCIDDLAANGNIDYVYIFSAIALFILLIACINFMNLSTARSANRAREVGIRKVLGS
                     PRKYLMAQFLTESVIVTMAGAVLALFLAWGLVPLFNTLSGKELTVTYHLVGRLLPVLL
                     AFVLVIGCLAGSYPAFFLSSFQPIDVLKGKLSTGFKGGRLRSFLVVFQFAISLFLITS
                     TLVVYRQIKYIQSRDIGFNRDHVLVVYNTGYLGSQATSFKQEIKQLSRVDNATLTSAL
                     PTANYGDYTSLFEDRSLDQKRAVHSQRWSVDEDYLNTLGMQLISGRNFSPQMPTDSSA
                     VIINEAAARMLTYANPLNQPLYAPADDFLKKISTYHIIGVIRDFNFQSLRQNVTPLIL
                     QLQPDPGALSIRIHAGADIPAVLAQVREKWKMLSPNRGFAYSFMEEDFDGLYRAEQRI
                     GQIFISFSSLAILIACLGLFGLVAYAAEQRTREIGIRKVLGAGIPTIVNMLSRDFIKL
                     VLLAILIACPLAWLAMHKWLQGFAYRVNISLWIFIFAALIALLIAVLTVSIQTIRAAT
                     ANPVKSLKTE"
     gene            complement(2765..3277)
                     /locus_tag="VKQ52_20855"
     CDS             complement(2765..3277)
                     /locus_tag="VKQ52_20855"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLZ89722.1"
                     /translation="MKSKVNIIPCLTSFGIILAATGCITGNHHKNEHQMSTTAQTAIF
                     PKGNPQPPEYFTGAAFLYPLLSKDKNNEFALGSVTFEPGARTHWHTHPKGQVLIVIEG
                     EGIYQEKDKPARHLKKGDVVNIPENVEHWHGAAAHSKFVHIAITNYKGEQNVVWLQPV
                     SEAEYQAANK"
     gene            complement(3287..4048)
                     /locus_tag="VKQ52_20860"
     CDS             complement(3287..4048)
                     /locus_tag="VKQ52_20860"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLZ89723.1"
                     /translation="MKIFEGKVALVTGAASGLGLATAKAFVEAGAAVALADWNDKAVQ
                     SAAQQLAEAGYTTLAIACDVSNDKQVEEMVSKTVSTFGRLDAAYNNAGVQNELAKAED
                     QTREDFDRVMGVNYRGVWSCMKYELKQMSKQGSGAIVNCSSIGGILGGARRGTYHGAK
                     HGVIGLTKSAALEYAAQGIRINSICPGLFQTSMSEQMIAAGQQEALDEMLKQVPAGRL
                     GRSEEIASAVLWLCSDGASMVVGHTLVVDGGYSIQ"
     gene            4200..5096
                     /locus_tag="VKQ52_20865"
     CDS             4200..5096
                     /locus_tag="VKQ52_20865"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ89724.1"
                     /translation="MEKVLRIDAVSEHDKFYHQENLHPLVSVIDFTGKTPEIYASKMN
                     FGFYAVYLKDVQCGDIKYGRNTYDYQDRTLVFVAPGQIINVNINKDYKPQGYALLFHP
                     DLIHGTPLGRHMDDYTFFSYESREALHLSEKERKIVLDCFSKIKYELEQGTDKHSKML
                     IASNIELFLNYCIRFYDRQFITRTDANRGNLEKFEHLLSDYFHSDKPQTIGLPTVSYC
                     AEQLHLSSNYFGDMVKKATGKTALEYIQLKVIEVAKEKVLDAGRSISEIAYELGFKYP
                     QHFTRVFKNGTGYTPNEYRHLN"
     gene            5120..5506
                     /locus_tag="VKQ52_20870"
     CDS             5120..5506
                     /locus_tag="VKQ52_20870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HLZ89725.1"
                     /translation="MKRYLYRMALAFSLFTVKGASAQEKNMIVQIARLQIDSAHLDEY
                     RAMLKEGVETAIRVEPGVLTLYAVYEKEHPTHVTVFEVYADQAAYEAHITSAHFLKYK
                     TGTQSMVKSLELVRVSPIALGSKEKQ"
     gene            5625..>7258
                     /locus_tag="VKQ52_20875"
     CDS             5625..>7258
                     /locus_tag="VKQ52_20875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89726.1"
                     /translation="MLKSYWTLAWRTLVRNKLYTLVNVLGLALGICACLVIYLIASYE
                     FSFDTFHPDRDRIFCVDAGMRGGHWNCVPGPMPAAMRREITGFQTVAAFQTYAASVTI
                     PGGPNGKPKSFGDDASIAIAEPQYFDIFRYRWLSGNAATSLTIPNSVVLTAEKARKYF
                     GDLDPDSIIGRTIYYRDSLVVRVTGVVADWTGNSDFALTQWISYATIPVSFLKNDIEL
                     DAWNKFNRSSQVMIRLANGVRPSQIDAQFPAFAKRHYGPDPAVAARLQPLSGIHFHQE
                     YGGAGRKASLPVLYTLMAVAGFILLLAIINFVNLSTAQSMQRAKEIGVRKVMGGSRSG
                     IALQFFTETLLVTVAAVGLAALMVRPVFSLFADLIPAGVHFRLDGATTLFLLLVIAAT
                     TLLAGFYPAKVLGNYQPTVTLKGITAVPGGGKGTLRRGLIVFQFTISLLFIIASLVIG
                     SQLRYALHADLGFKTDAIITLSPPRDNRSDMVAFQSDKNRVRRQATVETGNGAVLTDK
                     IRQLAGVAQVIREWRPPMGQMHSSFGGGGLKNSNEQP"
BASE COUNT         1663 a         1842 c         1883 g         1870 t
ORIGIN      
        1 gcaagggacg gccgctggtg tacggacaag ataaaatcca gtaccttagt cctgaacggc
       61 aactatcccc ttaaaaaggc acctgtatag ccgttctcag gatttattct ttcacctgta
      121 tagctatttt aggacgagac attttcgata aaaccgaaca catagttgta ttgaaatcgg
      181 acggtccggg gtgccagaat tgatgtaatc gattgatagc tagcttaaaa gtagtatggc
      241 actatcttgg cgcgaaacaa ctggggctta cagtcacccg tcaccaaaac aaaattcccg
      301 gccgtgttta caaactatct caaaaccgcc ttccgcaatc tcctgaaaaa taaaggcttc
      361 accgccctca atgtgctggg gctcacgctc ggtgtcgcta cctgcctgct gatcgtcttt
      421 tatgtgctgg atgaagtgag ttacgatcgg tacaatgaaa aggcagaccg gatctacagg
      481 atcaataaca tgatcaaatt cgggggtaat gagaacacct acgccggctc gcctgcaccc
      541 gctgccgccg ccctgaaaaa cgatttcccg gagatcgggc aggtcgtccg gttcatcaaa
      601 tccggcggga tccaggtgaa gaaaggcggt cagtatatcc aggaagacca tgccatcttc
      661 gcggattcca gccttttcgc ggtgtttacc ctgtctatga tcgacggcaa accggccact
      721 gctttgaggg atcctcattc ggcggtgatc acggaacaga cagccaaaaa atattttaac
      781 cggaccgacg tggtcggaca aacactcctg atcggtgata ccgatgccta taagatcacc
      841 ggcgtgatca gggatatccc ggctgaatcc catttccgat gcaatttttt cttctcgttg
      901 tctaccctgg cgtatagccg cgaagacgcc tggctggata ataatcttat tacctatgtc
      961 ctgttggaaa agggcgccga ccccgccagg ctggatgcta aattccccgc tttcatccgg
     1021 aagtacatgg ggccgcagct tcaaggtgcg ctgcacttaa ccttcgacga tttcgagcac
     1081 ggcgggaatt attaccggtt taagctcttt ccgttgacca gcatccatct ccggtcaaac
     1141 tgcatcgatg acctggctgc caacggcaac atagactatg tctacatctt ttcggctata
     1201 gccctattca tcctgctgat cgcctgtatc aatttcatga acctgtctac ggcccggtcg
     1261 gctaaccgcg cccgggaggt cggtatcagg aaagtgctgg gctcgccgcg gaaatacctg
     1321 atggcccaat tcctgacgga gtctgtcatc gtcacgatgg ccggcgctgt tctggcgctg
     1381 ttcctggcct ggggcctcgt gccgctgttc aatacccttt ccggcaaaga gttgacggtc
     1441 acctatcacc tggtaggcag gctgctgccc gtattgctgg cttttgtcct ggtcatcggt
     1501 tgcctggccg gctcatatcc cgccttcttt ctttcgtcct ttcaaccgat cgatgtgctg
     1561 aaaggaaagc tgtcgaccgg ctttaaaggc gggcgcctgc gaagcttcct ggtggtattt
     1621 cagttcgcta tttccctgtt cctgatcacc agcaccctgg tggtttaccg gcagatcaaa
     1681 tacatccagt ccagggacat cgggtttaac cgcgatcatg tgctggtggt atacaacaca
     1741 ggatacctgg gtagccaggc cacgtccttc aaacaggaga tcaaacagct gtcccgggta
     1801 gacaatgcca ccctgaccag cgccctgccg acagctaatt atggtgacta tacgtccttg
     1861 tttgaagacc ggtctctcga tcagaaacgg gccgtccatt cacagcggtg gagtgtcgat
     1921 gaagactacc ttaacaccct gggtatgcag ctaatatcgg gaaggaattt ctccccgcag
     1981 atgccgacgg attccagcgc ggttattatc aacgaggccg ccgcaaggat gttgacctat
     2041 gccaatccct taaaccagcc attgtatgcc cctgccgatg atttcctgaa aaagatcagt
     2101 acctaccata ttatcggggt gatcagggat tttaatttcc agtcccttcg ccaaaatgtg
     2161 acaccgctga tcctccaact gcagccagac cccggtgcac tcagcattcg catccatgcg
     2221 ggcgccgata tcccggccgt gctggcacag gtcagggaaa aatggaagat gctttcgccc
     2281 aaccgcgggt ttgcctattc ctttatggag gaagatttcg acggtcttta tcgtgcggag
     2341 caacgaatcg gtcagatctt tatctcgttt agctcgctcg ccatcctcat cgcctgcctg
     2401 ggtcttttcg gactggtggc gtatgccgcc gaacagcgga ccagggagat cggtatccgg
     2461 aaagtgctgg gggcggggat acccaccatt gtaaacatgc tgtccaggga ctttatcaaa
     2521 ctggtattgc ttgccatcct catcgcctgc ccgctggcct ggctggccat gcataaatgg
     2581 ctgcaaggct ttgcctaccg ggtgaatatt tctttgtgga tttttatctt tgccgctttg
     2641 attgccttgc tgatcgccgt gctgactgta agcatccaga cgatcagggc tgcaacggcg
     2701 aatccggtga agagtttgaa gacggagtaa ggtcaaagaa acgagtatcc ttccttatta
     2761 tttattactt atttgcggcc tgatactccg cttctgagac gggttgcagc cacacgacat
     2821 tttgctctcc tttgtagttt gtgatggcga tatggacgaa tttgctatgt gcagccgcgc
     2881 cgtgccagtg ctccacattt tccggtatat tgacgacatc cccctttttc aggtgccggg
     2941 caggtttatc tttctcctgg taaatccctt caccttcgat gacgatgagc acctgtccct
     3001 tggggtgtgt atgccagtgg gttcttgcgc caggctcgaa ggttacgctg ccgagcgcaa
     3061 attcgttgtt cttgtctttg gacaataaag gatagaggaa ggcagcaccg gtaaagtatt
     3121 ccggcggctg tgggttgcct ttcgggaata tggctgtttg cgctgttgtt gacatttgat
     3181 gttcgttttt atgatgatta cctgtgatac atccggtagc agctaatatt atgccgaatg
     3241 aggtcagaca gggaatgatg ttaacttttg acttcatacc gtctttttac tgtatgctat
     3301 accccccgtc tacgaccaag gtatgaccga ccaccatgct ggctccgtcg ctgcaaagcc
     3361 acaagacggc gcttgcgatt tcctcggacc ggccaagtcg gcctgccgga acttgtttca
     3421 gcatttcgtc aagcgcttct tgttgcccgg ccgctatcat ttgctccgac atcgaggttt
     3481 ggaagagacc ggggcaaatg ctgttgatcc gaataccctg agcggcatat tccaatgcgg
     3541 cgctctttgt taaaccaatg acgccgtgtt tggcaccgtg gtaggttccg cgccgggcac
     3601 cgcctaaaat accgccaatc gaagaacagt tgacgatggc gccgctgcct tgcttgctca
     3661 tttgcttaag ttcatacttc atacagctcc agactccccg gtagttgaca cccataaccc
     3721 ggtcgaaatc ctcccgcgtc tggtcctcgg cctttgccag ctcgttttgc acaccggcat
     3781 tgttataggc ggcgtccaat cggccaaacg tcgaaaccgt tttgctcacc atttcttcaa
     3841 cttgcttgtc atttgaaacg tcgcatgcaa tggccagtgt cgtgtatccc gcctctgcca
     3901 gctgctgagc tgcggactgt acagctttgt cattccagtc tgccaatgca accgctgcgc
     3961 cagcttctac gaaggctttt gccgtcgcga ggccgaggcc tgatgctgcg ccggttacca
     4021 gcgctacttt cccttcgaat attttcatga tctgtttttt ttacgctaca aaggtcggtc
     4081 gaccgggagg aacgacaggt atacaggata cagatttgcc taccattttt acaggttggg
     4141 ctggcggagt ggtacaagga gaagaataac tgctaaattt ggtttataaa atggataata
     4201 tggaaaaggt cttgagaatc gatgccgtga gcgagcatga taaattctat caccaggaaa
     4261 accttcatcc tctggttagt gtaattgact ttacggggaa gacaccggag atctatgcat
     4321 cgaaaatgaa ctttgggttt tatgcggtct atttgaaaga cgtacaatgc ggggatatta
     4381 aatatggtcg taatacctat gactatcagg accggacgct ggtatttgtt gcgccgggac
     4441 agattatcaa cgtgaatatc aataaggact ataagccgca gggatacgcg ctgcttttcc
     4501 atcccgatct gatccatggc accccgctgg gcaggcatat ggatgactat actttttttt
     4561 cttacgaatc cagggaggcg ctacaccttt cggaaaagga aagaaagatc gtcctggact
     4621 gtttttcaaa gataaagtat gaattggagc agggaacgga taagcatagc aaaatgctga
     4681 tcgcttccaa tattgaattg tttctcaatt attgtatccg gttttatgac cggcagttca
     4741 tcacccgcac tgacgcgaac cgtgggaatc tggagaaatt tgagcattta ttgagtgact
     4801 attttcattc ggataagcca caaacgatcg gtttgccaac ggtgagttat tgtgcagagc
     4861 agttgcatct ttcttccaat tattttggag acatggtgaa gaaagctacc ggtaagacgg
     4921 ccctggaata tatccagcta aaagttattg aagtagccaa ggaaaaggtt ttggatgcag
     4981 gacggtcgat cagtgaaatt gcgtatgagt tggggtttaa atacccacag cattttacac
     5041 gggtattcaa gaatggtacc ggctatacac cgaatgaata tcggcatttg aactgaagcg
     5101 tgtttaaatc tatttcgata tgaaaagata cctatatagg atggctcttg cattttcttt
     5161 atttactgta aaaggggcat ccgcccagga gaagaatatg attgtacaaa ttgccagatt
     5221 acaaatcgat tcggcccatt tggacgagta cagggccatg ctcaaagaag gtgttgagac
     5281 ggccattagg gtcgaaccgg gtgtactgac tttatatgcg gtatatgaaa aagagcatcc
     5341 tacgcacgta accgtttttg aggtgtatgc tgaccaggcg gcttatgagg cgcatattac
     5401 atccgcgcat ttcctgaagt acaagaccgg tacccaaagt atggtaaagt cgcttgagct
     5461 ggttagagtc agtcctatag cgcttgggtc gaaggaaaag cagtaaaaag cccgggatct
     5521 gcccgtagtc attccgcaag ttccaaaaaa tagcatatat taatatggaa tttgcgggca
     5581 tcttactcat agagtcacgc aaacctcctt ttaaaacctt aactatgctt aaaagctatt
     5641 ggacgctggc ttggcgaacc ctggttcgca acaagctata cacgctggtc aatgtcctgg
     5701 gattggcgtt gggtatttgt gcctgcctgg tcatttattt gatcgccagc tacgaattca
     5761 gttttgatac ctttcatccg gaccgtgacc gcatcttttg tgtggacgcc gggatgcgtg
     5821 gtggtcattg gaattgcgta cctggtccta tgccggccgc tatgcgccgg gaaatcaccg
     5881 gctttcagac cgtagccgcc tttcagacct atgccgccag cgtgacgata cccggcggtc
     5941 ccaatggaaa accgaagagc ttcggcgacg acgccagcat cgctatcgcg gagccacaat
     6001 acttcgacat ctttcgctac cggtggttaa gcggaaatgc cgcgacttcc ctgaccatac
     6061 ccaactcggt cgtgcttacc gcggaaaaag ccagaaaata ctttggcgat ctcgacccgg
     6121 acagcatcat cggccggacc atctattatc gggattcgct ggtcgtcagg gtaacagggg
     6181 tcgtcgccga ttggacaggc aacagcgatt ttgctcttac ccaatggatc tcttatgcta
     6241 ccatccccgt aagttttctg aagaacgaca tagagctgga tgcctggaac aagtttaacc
     6301 gttccagcca ggtcatgatc cgactggcaa acggcgtcag gccttcgcag atcgatgcgc
     6361 aattccccgc atttgccaaa cggcactatg gcccggaccc ggcggtcgcc gcccgtttgc
     6421 aaccgctttc gggcatccat tttcatcagg aatatggtgg tgcgggcaga aaggccagcc
     6481 tgccggtctt gtataccctc atggccgtgg caggctttat cctgcttctt gccattatca
     6541 attttgtcaa tttgtccacc gcgcaatcca tgcaaagagc gaaagagatc ggtgtccgaa
     6601 aggtgatggg cggcagcagg tcgggcattg cgctgcagtt tttcaccgag accctcctcg
     6661 taacggtagc ggcagtgggt cttgcggcgt tgatggtaag gccggtgttc agcctgtttg
     6721 ccgatttgat cccagcggga gttcatttcc ggttggatgg tgctacgact ttatttttgt
     6781 tgttggtaat cgcggcgacc accctccttg cggggtttta tccggcgaag gtgctgggga
     6841 attatcagcc caccgtgacc ctgaaaggga tcactgccgt gccaggcggc ggaaaaggga
     6901 cgctgcgcag agggcttatc gtttttcaat ttaccatatc gctgttgttc attatagcgt
     6961 ctcttgttat tggttcgcag ctgcggtacg cgctgcatgc ggacctgggt ttcaagaccg
     7021 atgcgatcat cacgctcagc cctccgcggg acaacaggtc ggatatggtg gctttccagt
     7081 cggataagaa tagggtccgg cgacaggcta ctgtggagac gggtaatgga gcggtgctga
     7141 cggataagat ccggcaactg gctggcgtag cgcaggtgat cagagagtgg aggccgccga
     7201 tgggccagat gcattcgtcg ttcggcggcg gcgggttgaa aaacagcaat gagcagcc
//
LOCUS       DATGFG010001101         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_621553, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001101 DATGFG010000000
VERSION     DATGFG010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621553"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /locus_tag="VKQ52_20880"
     CDS             complement(<1..1905)
                     /locus_tag="VKQ52_20880"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HLZ89727.1"
                     /translation="MVRITPVLTGLLLLVRATAQPTAQQPVDWVNPLMGTDSKPSLSN
                     GNTYPAIALPWGMNFWTPQTGVMGDGWQYTYGADKIRGFKQTHQPSPWMNDYGQFSIM
                     PVTGHLHLSEEDRASWFSHKTETATPYYYKVYLADPDVTAELTPTERTAQFRFTFPQS
                     DSSSIVVDAFDKGSWIRVIPEERKIIGYSTRQAGAPLTNFHNYFVIYVDRPIGFARTY
                     SDTVQQTGLETKAGHALAVIGFATTKGEQVHLRVASSFISTEQAELSLRREQGNEGFE
                     AIKEKARSTWNRTLGRIAVEGGSPAQQQTFYSCLYRMLFFPNKLYELDSQGRVMHWSP
                     YNGEVLPGYMFAGTGFWDTFRALYPFLNLVYPSVSREMQQGLVNDYKEGGWLPEWSSP
                     GYSDVMVGNNSASVVAEAWLKGLRGYDIETLYQALLHDANGEGPRAAGRRGAEYYNTL
                     GYVPYDVHINENAARTIEYAYDDFAIWQLGRALGKPASEIGVYRRRATNYRNLFDPST
                     GLMRGRNRDGSFESPFNPFKWGDAFTEGNSWHYTWGVFHDVRGLINLMGGERSFVAKL
                     DSVFTLPPVFDDSYYGFVIHEIREMQIAGMGQYAHGNQPIQHMIYLYDYAGEPWKTQY
                     WARETMNRLYK"
     gene            complement(1899..>2750)
                     /locus_tag="VKQ52_20885"
     CDS             complement(1899..>2750)
                     /locus_tag="VKQ52_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89728.1"
                     /translation="IADWVRKGGILIYCGSDDDPYQSVMEWWNSKGNHYTAPSQHLFQ
                     LLGISAATSGKLQPDPSTAGQQPDPSTAGQQTDPTTGSRQYKVGRGTVYLIRRDPKEF
                     VLQAAGDTAYFNLLKRAYETGAAGKLVTKNNLYLERGPYDIISVLDENPDTRPYVVPG
                     PVIDLFDPQLPVLPAKTVNPGEQSLLYDLRRVGDRTHPRVLASASRISDSTAKDKRFS
                     FLAKSPAKTSNSVRVLLPAQPTRTTVTDHDGQPLTDVQSSWDASSNTLYLGFPNSPDG
                     IKVDLQW"
BASE COUNT          485 a          722 c          926 g          617 t
ORIGIN      
        1 cttgtagagc cggttcatgg tctcgcgcgc ccagtattgg gtcttccagg gctcgccggc
       61 atagtcatac aaatagatca tatgctggat cggctggtta ccgtgggcat attgtcccat
      121 gccggcgatc tgcatctcgc ggatctcgtg gatcacgaag ccgtaatagc tgtcgtcgaa
      181 gacgggtggc agggtaaaga cggaatccag tttggcgacg aaggagcgtt ccccgcccat
      241 caggttgatg agcccccgga catcgtgaaa gaccccccag gtgtagtgcc agctattgcc
      301 ttctgtgaag gcatcgcccc atttgaacgg gttgaagggc gattcgaagg acccatccct
      361 gttgcgtccc cgcatcagcc cggtagaggg gtcgaagagg ttgcggtagt tcgtggcgcg
      421 gcggcggtat accccgatct cggaagcggg tttgcccagg gccctgccca gctgccagat
      481 ggcgaagtcg tcgtaggcat attcgatagt gcgagcggca ttctcgttga tgtggacatc
      541 gtagggtaca tagccaagcg tgttgtaata ctccgctccg cggcggccgg cggcgcgcgg
      601 gccttcccca ttcgcgtcgt ggagcagcgc ctggtagagg gtttcgatat cataaccccg
      661 caggcccttc agccaggcct ccgccaccac cgaagccgag ttattcccta ccatgacatc
      721 ggagtagccg ggactcgacc attccggcag ccagccgcct tccttataat cgttgaccag
      781 tccctgctgc atctccctgc tgacggaagg atagaccagg ttgaggaagg gatagagggc
      841 cctgaaggta tcccagaagc cggtgccggc gaacatatac ccgggcagga cctcgccgtt
      901 gtacgggctc cagtgcatga cccggccctg gctgtccagt tcatagagct tgttggggaa
      961 gaacagcatc cggtacaaac aggaatagaa tgtttgctgc tgtgcgggtg agccgccttc
     1021 cactgcgatg cggccgagtg tcctgttcca ggtggacctc gccttttctt ttattgcttc
     1081 gaaaccttcg ttaccctgtt cgcgccgcag gctgagttcc gcctgttccg tgctgataaa
     1141 ggaagaggcc acccgcaggt gtacctgctc gccctttgtc gtggcaaacc cgatgacggc
     1201 gagggcatga ccggcttttg tttcgagccc ggtctgttgt acggtgtcgc tgtaggtcct
     1261 ggcgaagccg atggggcggt cgacatagat gacaaaatag ttgtggaagt tggtaagcgg
     1321 cgcgccggcc tgtcttgtcg agtagccgat gatcttccgc tcttcgggga tgaccctgat
     1381 ccaggagcct ttatcgaagg catcgaccac gatggaggag ctgtcgcttt gcggaaaggt
     1441 gaaacggaac tgcgcggtgc gttccgtcgg tgtgagctcc gcggtgacgt cggggtcggc
     1501 gaggtagact ttatagtagt acggcgtcgc cgtctccgtt ttgtgcgaga accaggaggc
     1561 gcggtcttcc tccgacaggt gcagatgccc cgtcaccggc atgatgctga attgcccgta
     1621 gtcgttcatc cagggggaag gctggtgggt ttgtttgaag ccgcggatct tgtcggcgcc
     1681 ataggtgtat tgccagccgt cgcccatgac gccggtttgg ggtgtccaga aattcatgcc
     1741 ccagggcagg gcgatggcgg gataggtgtt gccgttggac agggaaggtt tggagtcggt
     1801 gcccatgagg gggtttaccc aatcgacggg ttgttgggca gttggttgcg cggtggcccg
     1861 gacaagcagc agcagaccgg ttagcacagg cgtgattctt accattgcag atcgactttt
     1921 atcccatcgg ggctgttcgg aaaaccaaga tagagggtgt tggaggaggc gtcccaggag
     1981 gactgtacat cggtgagcgg ttgcccgtcg tgatcggtga cggtcgttct tgtcggttgg
     2041 gcgggcagga ggacgcggac gctgttcgat gttttcgcag ggctcttcgc gaggaaggag
     2101 aagcgcttgt cttttgcggt gctgtcggag atgcgggagg ccgaggccag tacccgggga
     2161 tgggtcctgt cccctaccct gcgcagatcg tacaggaggg attgctcgcc ggggttgacc
     2221 gtttttgccg ggaggacggg gagctgtgga tcgaagaggt ctattaccgg tcccggtaca
     2281 acataaggcc gggtatcggg attctcgtcc aggaccgaga tgatatcata gggaccgcgt
     2341 tcgaggtaga ggttgttttt tgttaccagc tttccggcgg cgcccgtttc gtacgccctt
     2401 ttcagcaggt tgaaataggc agtgtcgccg gcggcctgga gcacgaattc cttcgggtcg
     2461 cggcggatga gatagacggt tcccctgccg accttgtatt gccggctgcc ggtggtggga
     2521 tcggtttgtt gtccggcggt ggagggatcg ggttgttgtc cggcggtgga gggatcgggt
     2581 tgtagtttgc cggacgtcgc cgcggagatc cccaggagtt ggaaaaggtg ctgggaggga
     2641 gcggtatagt ggttgccctt cgagttccac cactccatga ccgactggta cggatcgtcg
     2701 tcgctgccgc aatagatcaa gatcccgcct tttcgcaccc agtcggcgat
//
LOCUS       DATGFG010001102         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_295674, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001102 DATGFG010000000
VERSION     DATGFG010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295674"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VKQ52_20890"
     CDS             <1..1299
                     /locus_tag="VKQ52_20890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLZ89729.1"
                     /translation="GKTIQALSFLQHFRDEHGRLSALVVCPTTLMFNWENEIRKFTPA
                     LTYHIHHGGDRTRNKEILSQYNIIITTYGTLRSDIKLLLEMNFDYAVLDESQAIKNPG
                     SKVTKAACLLQARHRLCMSGTPLQNNTFDIFAQMNFLNPGMLGSLEFFRQEFAVPIDK
                     FGEQDRKEHLRKLLYPFILRRTKEQVAKDLPEKTETILFCEMEDEQRKVYEAYRNDYR
                     DKILGTIETQGIQRSQLTILQGLMKLRQICDSPAILNETDKYPNHSIKLDELAREITE
                     NIGDHKALIFSQFLGMLALIRERLKELDVKFEYFDGSTSAVERERAIQSFQNDDTCRV
                     FLISLKAGGVGLNLTAADYVYIVDPWWNPAVEQQAIDRTHRIGQTKNIFAYRMICKDT
                     IEDKILQLQEKKRILAKDLIADDDGFVKSLTRADVEYLFS"
     gene            1451..>2438
                     /locus_tag="VKQ52_20895"
     CDS             1451..>2438
                     /locus_tag="VKQ52_20895"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HLZ89730.1"
                     /translation="MKKALLFLLILSLAFATHSFSQSNTRGSVKGVLVDTAGGRQPLV
                     NATISITPLGGDSTDAEYTVSGKGGVFQIKGIRVGQYKLLITYEGYQHIDRRVNISDS
                     TPVVDLATLFMNRADNMLAEVVIQRPPLGIKKDTVEYNAGSFATKPNAVAEDLLKKLP
                     GVTVDNSGTVTAHGETVQRILVDGKRFFSDDPKLATRNLPPDIIDKIQVFDDLSDQSK
                     FTGFDDGNRVKTINIVTKKNSRKGYFGRVVGAGGTNEDYDESVNMHRFDGNQQLSLLG
                     QGNDINKQNFTPQDIFGNNGGGGGGGRRGGNGGGGGGFGAATSPQSTGVTTVW"
BASE COUNT          583 a          637 c          721 g          497 t
ORIGIN      
        1 ggtaagacca tccaggccct gtccttcctg cagcatttca gggatgagca tggccggctc
       61 agcgcccttg tggtctgtcc caccacgctg atgttcaact gggagaatga gatccggaag
      121 tttactccgg cattgaccta tcatatccac catggcggcg accggacacg gaacaaggag
      181 atcctttccc aatacaatat catcatcacc acttatggga ccctgcgcag cgatatcaag
      241 ctcctgctgg agatgaactt cgattatgcc gtgctggatg agtcgcaggc gatcaagaac
      301 ccgggcagca aggtgaccaa ggccgcctgt ctgctccagg cccgtcaccg gctttgtatg
      361 agcggtacgc ccctgcagaa caataccttt gacatctttg cccagatgaa cttcctcaac
      421 ccgggtatgc tggggagcct cgagttcttc cgccaggagt ttgccgtgcc gatcgacaaa
      481 ttcggcgagc aggaccgcaa ggagcatctg cggaagctgt tgtatccgtt catcctccgg
      541 cggacaaagg aacaggtggc caaggacctg cccgaaaaga cggagaccat cctcttttgc
      601 gagatggaag acgaacagcg gaaggtgtat gaggcgtaca ggaatgacta ccgggataag
      661 atcctgggga cgatcgagac acagggcatc cagcggagcc aattgaccat cctgcagggc
      721 ctgatgaagc tgcggcagat ctgtgactcg ccggccatcc tgaacgaaac ggataaatat
      781 ccgaatcatt cgatcaagct ggacgagctg gcccgggaga taaccgagaa tatcggggat
      841 cacaaggcgc tgatcttttc ccagttcctg gggatgctgg ccctgatccg ggaaagactg
      901 aaagaactgg atgtcaaatt cgaatatttt gacggaagca cttccgccgt ggaacgcgag
      961 cgggccatcc aaagcttcca gaatgacgat acctgccggg ttttcctcat ctccctgaag
     1021 gcgggtgggg tgggtctgaa cctgaccgcc gccgactacg tctatattgt cgacccctgg
     1081 tggaatcccg ccgtggaaca acaagccatc gaccggaccc atcggatcgg ccagaccaag
     1141 aacatttttg cctaccggat gatctgtaag gatacgatcg aggacaagat cctccaattg
     1201 caggagaaga agcgcatcct ggccaaggat ctcatcgccg atgacgatgg attcgtcaag
     1261 agcctgaccc gcgccgatgt agaatacctg tttagttaga gtatttaccg ataagcctga
     1321 aaatatgtcc ccggggttga ccctattgtc gccttaattt taccactggt acaggcggct
     1381 atatgtgcgc tgcgtgccgg ttcttacttt agcagactat ttgcaaccag caatatatca
     1441 aagaccggct atgaaaaagg cattactttt tttgttgata ctctccctcg catttgcaac
     1501 gcattcattt tcacagtcta atacccgggg ttcggtaaaa ggcgtgttgg tggacactgc
     1561 gggcgggcga cagccgcttg tcaatgccac gatctcgata acaccgctgg gcggggactc
     1621 caccgacgcg gaatatacgg tctccggcaa gggcggggtc ttccagatca agggcatccg
     1681 ggtcgggcag tataaactgc tgatcactta tgaggggtat cagcatatcg acagaagggt
     1741 caatatttcc gatagtacgc cggtggtgga cctggccacg ctgtttatga accgggccga
     1801 caacatgctc gccgaggtgg tgatccagcg tccaccgctg ggcatcaaga aggacacggt
     1861 agagtataat gcggggtcgt ttgcaaccaa gccgaatgcg gttgcggagg atctgttgaa
     1921 gaaactgccg ggtgtgacgg tcgacaacag cggtacggtg acggcccatg gcgagacggt
     1981 gcagcggata ctggtcgacg gaaaacggtt ctttagcgac gatcccaaat tggcgacgcg
     2041 gaacctgccg ccggatatca tcgacaagat ccaggtgttt gacgacctga gcgatcagag
     2101 caagttcacc gggttcgacg atggcaaccg ggtgaagacc atcaatatcg ttacgaagaa
     2161 gaacagccgg aagggatatt tcgggagggt ggtcggtgcg gggggcacga acgaggacta
     2221 tgatgaaagc gtcaatatgc accggttcga cggcaaccag cagctctcgc tccttggcca
     2281 gggaaatgat atcaacaagc agaattttac gccccaggat atctttggca acaatggcgg
     2341 cggtggtggt ggtggacgcc ggggcggcaa cgggggcggc ggcggtggtt ttggcgctgc
     2401 cacgagcccg cagtccacgg gtgtgacgac ggtctggg
//
LOCUS       DATGFG010001103         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_519732, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001103 DATGFG010000000
VERSION     DATGFG010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519732"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VKQ52_20900"
     CDS             complement(<1..388)
                     /locus_tag="VKQ52_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89731.1"
                     /translation="MVLRSSILKVTTYFDLFNYPLTPEDICRFLDIEASQEAVREEVE
                     ALAQEGRLFRTGPFYSLQNDPTLAAKRLRGEVHADELLRIANRGARLLYQFPFVRGVC
                     ISGSLSKRCADEKADIDYFIITRANRL"
     gene            472..978
                     /locus_tag="VKQ52_20905"
                     /pseudo
     CDS             472..978
                     /locus_tag="VKQ52_20905"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            complement(1028..1900)
                     /locus_tag="VKQ52_20910"
     CDS             complement(1028..1900)
                     /locus_tag="VKQ52_20910"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HLZ89732.1"
                     /translation="MRKYLILFASMAVGHALFCQDGPITVQKGATVHGPESGSLLIIG
                     GNVGSTASIWNKFTELAGGRERAHIVVITAASGDSAAYDTASVNTVKRQTGIADVVLL
                     HTHDLKTANSESFIAPLKRATGVYFVGGRQWRIADCYLNTLTHQAFFDVLRRGGVIAG
                     SSAGASIQGSFLWRGDTKGPEVLIGDHTQGLGFLKNSVIDQHVLRRNRQFDLVNLIER
                     APQLIGIGLDEATAVLVQRDTLQVIGRSYVAIYDHGGSVPGPFFFLSEGQKYDLKERK
                     VIRAPRQAAAEEED"
     gene            complement(1897..2940)
                     /locus_tag="VKQ52_20915"
     CDS             complement(1897..2940)
                     /locus_tag="VKQ52_20915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLZ89733.1"
                     /translation="MRYGYWMPVFGGWLRNVEDEHMQPTWDYVKRLAIRSEEIGFDLS
                     LIAELNLNDIKGEQAPSLDAWSTAAALAAVTHKQELMVAVRPTFHNPALLAKQAANID
                     HISNGRLSLNVVSSWWRDEATKYGVGFEQHDDRYARTAEWLTVLDGVWQQDGFSFSGK
                     YYRVDNNTLQPKPISYVSRPRPAIYAGGESETAKDLISSTCDGYVMHGDEPATIGKRI
                     RDMKERRERKGLPPMQYGVAAYSIIRDNDAAVKKELERITNVQPGSAGYNNYQQWLAG
                     TQLEQKVSLEDYSVSNRGLRSGLIGTPAEVEDRIGAFAAEGVDFFLLQCSPQLEEMER
                     FADTIIRKEYISA"
     gene            complement(2937..3929)
                     /locus_tag="VKQ52_20920"
     CDS             complement(2937..3929)
                     /locus_tag="VKQ52_20920"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89734.1"
                     /translation="MSPIAIYHEHPDWFKPLFEELNNRGIPYVRLNPAAHQFAIEAPA
                     PDYSLFFNRMSPSAYLRDGIQGIFFTLNYLRHLEYAGIPIVNGYKAFVNETSKALQLE
                     LMQSLGIKYPRARVVNHPSQLVAAAEGLRFPVVVKANIGGSGAGITKYHTLEDLRAAA
                     EGGQMDFGVDHTALLQEFIPARGGYITRVETLGGKFLYAIRVHLTGDTFNLCPADICQ
                     TSAGVELVRNACAIEAPKSGLKVEGYTPPPAVIKAVETMVQQSGIDVGGIEYIIDDRD
                     GELLYYDVNALSNFVADAVNVIGFNPHQRLVDYLAERAGAQQGKKAKNLQSAAL"
     gene            complement(4022..4873)
                     /locus_tag="VKQ52_20925"
     CDS             complement(4022..4873)
                     /locus_tag="VKQ52_20925"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLZ89735.1"
                     /translation="MRTFLFLCGFFLFVSGVRAQIPAGDGEGYFCGICGGDCDNLVFT
                     KPGVCPHCGMPLLRQTYAQRREIMKNYHNQMTICFYLQDGVEVLDFAGPMEVFSYAGF
                     KVFTVSRKKEPIMTQGILKIMPDYGIDDAPPADILAFFGGNAGPASVDPAVMAWIHQR
                     QATAPYFFSVCTGAFILGRAGILDSLSATTFHSSIESLRQALPKTKVLANVRFVDNGR
                     VITTAGISAGIDGALHLVSRLKGEEVAREVAAYMEYDKWVPNQGLVVKKNHLANDGPS
                     DSEPPVR"
     gene            complement(4939..>5173)
                     /locus_tag="VKQ52_20930"
     CDS             complement(4939..>5173)
                     /locus_tag="VKQ52_20930"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ89736.1"
                     /translation="ALIHTSPRNLTRLFKAATGVTVGQYLEKLRVEKAVHLLRQRGKI
                     AAIPRQCGLQSANQLRLLVKKHTGRLPSELSLS"
BASE COUNT         1168 a         1600 c         1292 g         1113 t
ORIGIN      
        1 aaagccggtt ggcccgggtg atgataaaat aatcgatatc ggccttctca tcggcgcaac
       61 gcttggacaa agaaccggat atacataccc cccggacaaa cgggaattga tagagcagcc
      121 ttgcgccgcg gttggcgatg cgcaaaagct catccgcatg gacctccccc cggagccgtt
      181 tcgccgcaag ggtgggatcg ttttgcaagg aataaaaagg ccccgtccgg aatagccttc
      241 cctcctgcgc cagcgcctcc acctcctccc tgacagcctc ctgcgaggcc tcgatatcga
      301 ggaaccggca aatgtcttcg ggcgtcaggg ggtagttgaa aaggtcgaaa tacgtggtca
      361 cctttaatat gctcgatcgt agtaccataa aaaaaagaac gcgccccgga aggcatcggt
      421 tgcttgacta tccaggcaac ctttccgatc ccggctgcgt ttgccattac gatggctata
      481 ttgaaaagag cattcgacct cctggtctcc accctcgtga tcctgagcgt gctgagttgg
      541 tttctgccgc tgctggctct tatgataaga cgggattctc ccggtccggt attcttcgtg
      601 cagaagaggg tagggaagga cggtaaacta tttcgctgtt ttaagttgcg gagtatggtt
      661 gttaatccac atgccgacca gcacccggtg gcaggagacg atggccgcat cactccactg
      721 ggtagctggc tccggctatc gcacctggat gaacttccgc aattttttaa tgtgtttctg
      781 ggttcgatga gtctcgtagg gcccagaccc tatatgccgg ccgactgccg gcgatttgcc
      841 acgatcatcc ccgaccaggc cttccgggaa aaggtaaaac ccggcatcac cggtatggcc
      901 caggcaaaag gcctgcatgg catcgtatgc gaccggaaca cgctcctgca gcgctatcaa
      961 tgggacgcct ggtatgtcga caataaaagc ttcggcctgg atatccgtat cctgtggacc
     1021 acgttcctca atcttcttcc tctgcagcgg cctgccgcgg cgcccggatc accttccgct
     1081 ccttcagatc atatttctgt ccttcactta gaaaaaagaa aggccccggt acactacctc
     1141 cgtgatcgta aatagcgaca taggatctgc cgatcacctg cagggtatcc ctttgcacca
     1201 gcaccgcggt agcctcatcc agcccgatgc cgataagctg cggcgccctc tcgataagat
     1261 tgacaagatc gaattgccgg ttgcgccgga gcacatgctg atcgataacg gagttcttca
     1321 ggaacccaag cccctgggtg tgatcgccga taaggacctc cggtcccttc gtatctcccc
     1381 gccacagaaa agagccctgt atcgaggcgc ctgccgagct gcccgcaatg accccacccc
     1441 gcctcagcac atcgaaaaat gcctggtggg tcagcgtatt cagatagcaa tctgctatcc
     1501 gccattgcct gccgccgaca aaataaaccc ccgtggcacg tttcagcggc gcgataaacg
     1561 attcgctgtt ggccgtctta aggtcatggg tatgcagcag caccacatcg gcaatccccg
     1621 tctgtctctt cacggtattg acagaggccg tatcataagc cgcgctgtcc cccgaagccg
     1681 ccgtgatcac cacgatatgg gccctctctc tgcccccggc caattcggtg aacttgttcc
     1741 agatggacgc cgtcgacccg acattcccac cgatgatcag cagcgaaccg ctctccggcc
     1801 catgaacggt cgctcctttc tgcaccgtga taggaccatc ctgacaaaac aaagcatggc
     1861 cgacggccat gctcgcaaat aagatcagat attttctcat gctgatatat attcttttct
     1921 aattatagta tccgcgaacc gctccatctc ctccaattgc ggactgcatt gcaacaggaa
     1981 gaaatccacc ccttccgccg caaatgcccc gatacggtcc tccacctccg ccggcgtacc
     2041 gataagcccc gaacgaagcc cgcggttgga aacggaatag tcctcaagag agaccttctg
     2101 ttccagctgc gtcccggcca gccattgctg gtagttgtta tagccggcac tgcccggctg
     2161 cacattggtg atccgctcca attctttctt cacggcggca tcgttgtccc ggatgatgct
     2221 gtaggcggcg accccatact gcatgggcgg caaccccttc ctttcccttc tttccttcat
     2281 atccctgatc cgtttgccga tggtggccgg ctcgtcgcca tgcatcacat aaccatcaca
     2341 ggtagacgat atgagatcct ttgccgtctc cgactcgccg ccggcataga tggcaggcct
     2401 cggccgggaa acgtaagaga taggctttgg ctgcaaagta ttattatcca cccggtaata
     2461 tttcccgcta aaactaaatc cgtcctgctg ccagacgccg tccagcaccg tcagccactc
     2521 ggccgtccgg gcataacggt catcatgctg ctcgaacccg accccgtact tggtggcctc
     2581 atcccgccac cacgaagaga ccacattgag ggaaagcctc ccgttgctga tatgatcgat
     2641 atttgccgcc tgcttggcca gcagggcggg attgtgaaag gtgggccgca cggcaaccat
     2701 cagctcctgc ttgtgcgtaa cggccgccag cgcagccgcc gtcgaccagg catccagcga
     2761 aggcgcctgc tcccccttga tatcattcag gttgagttcg gcgatcagcg aaagatcgaa
     2821 cccgatctcc tcacttcgta tcgccagcct ctttacatag tcccaggtgg gctgcatgtg
     2881 ctcgtcttcc acattgcgca gccagccgcc aaaaacgggc atccagtatc catatctcat
     2941 aatgctgcgc tttgtaggtt tttcgctttt ttaccttgtt gggctcctgc acgctccgcc
     3001 aggtaatcca ccagtcgctg gtggggattg aagccgatca cattcaccgc atccgcgaca
     3061 aaattcgaga gggcattcac atcataatat aacaactccc catcgcggtc gtcaatgatg
     3121 tattcgatgc cgcccacatc gatcccggac tgctgcacca tcgtctccac ggccttgatg
     3181 acggccggcg gcggcgtata cccctccacc ttcagaccgc tcttcggcgc ttcgatggcg
     3241 caggcattcc gcaccagctc tacgccggcg ctggtctgac agatatcggc gggacaaaga
     3301 ttgaaggtgt ccccggtgag atggacgcgg atggcataaa ggaattttcc acccagcgtt
     3361 tcgacccggg tgatataccc gccgcgcgcc gggatgaact cctgcaacaa cgccgtatgg
     3421 tcgacgccaa aatccatctg gcccccttcg gcggcggccc gcaggtcttc taacgtatga
     3481 tacttggtga tcccggcgcc actgccgccg atattggctt ttacgacgac gggaaaacgc
     3541 agtccttccg cagccgcaac cagttgcgac ggatggttca cgacacgggc cctgggatat
     3601 tttatcccca gcgattgcat cagctccagt tgcagtgcct tcgatgtctc atttacaaac
     3661 gccttgtatc cgttcactat cgggataccg gcatactcca ggtgccgtag ataattcagg
     3721 gtaaaaaaaa tgccctgtat cccatcccgc agataggccg aagggctcat ccggttaaag
     3781 aaaagggaat aatcgggcgc cggcgcctcg atggcgaatt gatgggccgc cgggttcagc
     3841 cggacatacg gtatccccct gttgttcaat tcttcaaaaa gcggtttgaa ccagtcggga
     3901 tgttcatgat aaatggcgat gggtgacata gggcaaatat agcccaaaat aaagtccttg
     3961 aacggcgcta atttaattat tagactccct ggcaacgacc aatccttgac ccggaatcca
     4021 cctagcggac cggaggttcg ctgtccgaag gaccatcatt agccaaatgg ttctttttta
     4081 ctaccaatcc ttgatttgga acccatttat catattccat atacgccgcc acttcccggg
     4141 ccacctcttc cccttttaac cggctcacaa gatgcaatgc cccgtctatc cccgcggaaa
     4201 taccggccgt agtgattacg cgcccattat ccacgaaccg gacattcgcc aacaccttcg
     4261 tcttcggtaa tgcctgccgc agcgactcga tactggaatg aaacgtggtc gcactcagac
     4321 tgtcgaggat acccgccctc cccaggataa aggcccccgt acaaaccgag aagaaatagg
     4381 gcgccgtcgc ctgccgctgg tggatccatg ccatcaccgc aggatcgacc gaagcgggac
     4441 ccgcattgcc gccaaaaaaa gcaaggatat ccgccggcgg cgcatcgtcg ataccatagt
     4501 ccggcatgat cttcaaaatg ccctgggtca tgatgggctc cttcttccgc gaaacggtga
     4561 agaccttgaa accggcatag gagaatactt ccatcggtcc ggcaaaatcg agtacctcga
     4621 ccccgtcctg taaataaaag cagatagtca tttgattatg gtagtttttc atgatctccc
     4681 tccgctgcgc ataggtctgc ctgagcagcg gcatcccgca atgcggacat acccccggct
     4741 tggtgaatac caggttatca caatccccac cacagatgcc acagaaataa ccctcgccgt
     4801 cgccggccgg gatctgagca cgcaccccac tgacaaaaag gaaaaagccg cataggaaaa
     4861 gaaaggtccg catagaaaag aagtttccgc aaaactactc ttacagcccg gataaaagga
     4921 ggacatccag gcgtaggatc aggacaaact cagttcgctc ggcaaccggc ccgtatgttt
     4981 cttcaccagc agccgcaact ggttcgcgct ttgcaacccg cattgccgcg gaatagccgc
     5041 gatcttccca cgctgacgca acagatgaac ggccttctct acgcgcagct tttccaggta
     5101 ctgcccgaca gtaacgccgg tggcggcctt gaagagccgg gtaagattac gcgggctggt
     5161 gtggatcaac gcc
//
LOCUS       DATGFG010001104         3578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1435271, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001104 DATGFG010000000
VERSION     DATGFG010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435271"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VKQ52_20935"
     CDS             <1..443
                     /locus_tag="VKQ52_20935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ89737.1"
                     /translation="LSARYQFTSWLYANFDINFSKARDEEAPKGSDYLPLSVPLSGTG
                     GLSFKLPNGINGGLSYRYMKDRPANADNSLVAKGYFLTDLTANYTKRKYEIGLEIQNL
                     FNSKWNEAAFEVTSRMRNEPAPVDDVSFTPGTPFFARLKLAVFF"
     gene            complement(513..1220)
                     /locus_tag="VKQ52_20940"
     CDS             complement(513..1220)
                     /locus_tag="VKQ52_20940"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ89738.1"
                     /translation="MRQIAFFLVFALPGFTGFAQQFLTGKVLKKETKETLSSVSIYNR
                     TQRAHKLSDEEGNYRIIAQPGDIVIFSCVGYHADTISVSEKILGAEFHILLDLRPVSL
                     QPVTVGSLSNYQLDSLERRQVYSWIYEQEPQPVVERQRQGDGVGVELNVIPHGSSEVR
                     ERLRLKKRILREEQQYYVSFRFSPDYISRLTHLGGDSLQQFMVRYRPTYDFCRKAASV
                     DILVYINDNFKKFQKRE"
     gene            1374..3344
                     /locus_tag="VKQ52_20945"
     CDS             1374..3344
                     /locus_tag="VKQ52_20945"
                     /inference="COORDINATES: protein motif:HMM:NF039200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HLZ89739.1"
                     /translation="MKRRSARLMYLFAFLLFHSTQAQHQQRLNGAFSILPLLDSCNVS
                     WDEPGPGSAQSMPIGNGDIGLNLWVEKNGDLVFYISKTDAWGAETRPEWDSWMKEGGV
                     LMKLGEVRISLTPSPFNGNTPFHQVLRLHDGEISIKEEEASFRVWVDANNPVIRVEAN
                     SPRPAGIKVSLNDWRLGEGDSIIPAEKDRLTWYHENRATTDSHLAGLVFGATIKAKGL
                     LRKDATTLQSGPANGSKLISIYPLTKAPVSTAALSARHPGHAAGSSPGVPAVSLVPSW
                     HSQLERQINTIDRLALEQTRTAHRTWWDQFWHRSWVFVHGDQQADATTKGYVLQRYIT
                     ACAGRGAFPIKFNGSIFVVDNPAWRSGGHNVPMSADFRAWGGQYWFQNTRAMYWPRLM
                     AGDFDLMLPLFNMYAKMLPDNTALVKKYYNHDGAYFQETTPFWGGLPYMGPEVEALYT
                     HHYFTPILELSMMMLDYYDYTGDTSFARNTLLPIASAGTTFFDKHFGRDIMGKLLLDP
                     DNSIEMFWKVRDPAPDIAALHAILPRLIHLPGTLVDAAQRDAWESSSPNCLPCPSAPA
                     LITSPSCCPIPVRKPLSHLTRRIPSSTPFIPIGSTDFTDPISKWPVPLFSIESSAIGD
                     AGSRTPSRRLCWGSPIWPGNILLSISPVKTLL"
     gene            3341..>3578
                     /locus_tag="VKQ52_20950"
     CDS             3341..>3578
                     /locus_tag="VKQ52_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89740.1"
                     /translation="MKFPAFWAKGNDYMPDEDNGGNGENGLQQMLLQTEGKKILLLPA
                     WPKDWDVDFKLNAPYRTTVAGRVVNGRLTDLVVTP"
BASE COUNT          830 a         1004 c          904 g          840 t
ORIGIN      
        1 atctgtccgc ccgttaccag ttcaccagtt ggctttatgc caatttcgat attaatttct
       61 ccaaagcccg ggatgaagag gctccgaaag gaagtgacta tcttccttta tctgttccct
      121 tgtccggcac cggtggcctg agcttcaagc tacccaatgg catcaatggg ggcctgagct
      181 accggtatat gaaggaccgc cccgccaacg cagacaactc cctggtagcc aaaggatatt
      241 tcttaaccga cctgacggcc aattacacga aacgtaaata tgagatcggc ctggagatcc
      301 agaacctttt caattcgaaa tggaatgagg cggcatttga agtaacatcg agaatgagaa
      361 atgagccggc gccagtggat gatgtgagct ttacaccagg gactcccttt tttgccaggc
      421 tgaagctggc agtatttttt taggccattt tacaacatat tgctgcagag gacaagctgg
      481 ctgagagggc tcccggaggg gtagggggct gtttattccc tcttctgaaa ttttttgaaa
      541 ttgtcgttga tatagaccag gatatccacg ctggctgcct ttcggcaaaa atcgtaggta
      601 ggacggtagc gcaccatgaa ttgctgcagc gagtcgcccc cgagatgcgt gagacgggaa
      661 atatagtcag gggaaaagcg gaagctcaca tagtattgct gctcctcccg caaaatgcgt
      721 tttttcagcc tcaggcgttc gcggacttcg gaggagccat gcgggataac attcagctcg
      781 acgcccacac catcgccctg acgctggcgc tcgaccaccg gctggggctc ctgctcgtag
      841 atccagctat atacctgtct gcgctccagg ctgtccagtt ggtaattgct caggctgcct
      901 accgttacag gttgcagcga aacaggccgc aaatccagca ggatgtgaaa ttccgcgccc
      961 aggatcttct cagagaccga aatggtgtcc gcatggtagc cgacacagga aaatatcacg
     1021 atatcacccg gctgcgctat gatcctgtaa ttgccctcct catccgaaag tttgtgcgcc
     1081 cgctgcgtcc ggttatatat acttacggaa ctcagggtct ccttggtttc tttcttaagg
     1141 acctttccgg taaggaattg ttgggcaaaa cccgtaaacc cgggcagggc gaaaactaaa
     1201 aaaaacgcga tttgtctcat attagaatgc taagttagga attaaccccg cgattatgat
     1261 tttattaatc attcccttaa cttcaccggg ctatttttgt aacagacagt gatgcccctt
     1321 cgggcgtaga aggctttgcc ttcctatatt gtttgccaac actccctcct tatatgaaga
     1381 gaagatcagc ccggttaatg tatttattcg cttttttact atttcactcc acccaggccc
     1441 agcatcaaca acggctgaac ggagcttttt ccatcctgcc cctgctggat tcctgtaatg
     1501 tttcctggga tgagcccggc cccggatcgg cgcagtccat gcccatcggc aatggcgaca
     1561 taggactgaa cctgtgggtg gaaaaaaatg gcgacctggt attttatatc agtaagactg
     1621 atgcctgggg cgcagagaca agacccgaat gggacagttg gatgaaagaa ggcggcgtgc
     1681 ttatgaaatt aggggaagtg cgtatttcac tgactccctc cccgtttaat ggaaatacac
     1741 ccttccacca ggtcctccgt ttgcacgacg gggaaatatc gatcaaagaa gaagaggcct
     1801 cattccgcgt ctgggtcgat gccaataacc ccgtgatcag ggtcgaagcc aacagccccc
     1861 ggcccgcagg cataaaagtc agtctgaatg attggcgttt aggcgagggt gattccatta
     1921 ttccggcaga gaaagatcgg ctcacctggt accatgagaa ccgcgcgacc accgactccc
     1981 acctggccgg ccttgtcttc ggcgctacta tcaaggcaaa aggtctctta cggaaggatg
     2041 caacgaccct ccaatcagga ccggctaatg gatcgaagct catttctatc tacccgctga
     2101 caaaagcacc ggtctccact gcggctcttt cagcccgaca tcctgggcac gccgccggat
     2161 cttctcccgg cgtgccggct gtttccctcg ttccgtcctg gcacagccag ctcgaacggc
     2221 agatcaacac cattgacagg ttagccctcg agcaaacccg taccgcccat cgcacctggt
     2281 gggaccagtt ctggcatcgc agctgggtct ttgtccatgg cgatcagcag gctgatgcta
     2341 ccacaaaggg ctacgttctt caacgctata tcacggcctg cgccggccgg ggtgcctttc
     2401 ctattaagtt caacggctct atctttgtcg tcgataaccc cgcatggcgg tcaggcggcc
     2461 ataacgttcc catgagcgcc gacttccgcg cctggggcgg acagtactgg ttccagaata
     2521 cccgcgccat gtattggccc cgcctcatgg ccggtgattt cgatctgatg ctgccgctgt
     2581 tcaatatgta tgcaaagatg ctccccgata atactgcgct ggtaaagaag tattataacc
     2641 atgacggcgc ttatttccag gagaccaccc ccttctgggg agggctccct tatatgggac
     2701 ccgaggtaga ggccttgtat acccatcact atttcacacc catcctggag ctcagcatga
     2761 tgatgctgga ctattacgat tatacgggcg acacctcatt cgcaaggaat acgctattgc
     2821 ctatcgcttc cgcgggcaca acctttttcg ataagcattt tggaagggat atcatgggga
     2881 aattgttact ggaccccgat aattctatcg agatgttctg gaaggtccgc gatccggcac
     2941 cagatatcgc cgcattacat gcaatccttc cccggttgat ccacctgccg ggcactctgg
     3001 tcgatgccgc tcagcgcgat gcctgggaaa gctcctcgcc gaattgcctc ccctgcccat
     3061 cggcgccagc cctgataaca agcccatcct gctgccctat accggtccgc aaaccgctaa
     3121 gtcatttaac tcggagaatc ccgagctcta cgccatttat ccctatcgga tctacggact
     3181 tcaccgaccc gatctcgaaa tggcccgtac cacttttctc catcgaaagt tcagcgatcg
     3241 gggatgctgg gtccaggacc ccatccaggc ggctatgctg gggttcgccg atctggccag
     3301 ggaatatact gctttcaatt tcacccgtaa agacccttct ttgaaattcc cggctttctg
     3361 ggccaaaggg aatgactata tgcccgatga agataacggc ggcaacggtg aaaatggctt
     3421 gcagcaaatg ctcctgcaga cggaaggaaa gaagatcctg ctgttgccgg catggcccaa
     3481 ggactgggat gtcgacttca agctcaatgc tccgtacagg accaccgtag ccggcagggt
     3541 agtgaatggc aggctgactg acctggtggt aacccccg
//
LOCUS       DATGFG010001105         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1435318, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001105 DATGFG010000000
VERSION     DATGFG010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435318"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VKQ52_20955"
     CDS             complement(<1..268)
                     /locus_tag="VKQ52_20955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ89741.1"
                     /translation="MKVRLVSVLVKDQEKALRFYTEVLGFVKKKEVPMGEHKWLTVVS
                     GEEPDGVEVVLEPLGFPPAVVFQRELFAAGIPWTAFYAEDVEAEY"
     gene            617..>2091
                     /locus_tag="VKQ52_20960"
     CDS             617..>2091
                     /locus_tag="VKQ52_20960"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLZ89742.1"
                     /translation="MTQYDAIVIGSGISGGWAAKELCEQGLTTLLLERGRPVEHLKDY
                     PTATKNPWDFPHRGQLPLKVLDDNPIVARCYAYGEATQHFFVKDKEHPYVQEKPFDWI
                     RGYQVGGKSLLWARQTQRWSPFEFEGPARDGIAIPWPIGYNDLAPWYSHVEKFAGISG
                     NKDGLPQLPDGEFLPPWEMNCVEQHIKEKILQRYPDRPVIIGRCAHLTKPQPIHLEQG
                     RGQCQARSLCERGCPFGGYFNSNSTTIPWAQRTGKLTLRPYSVVHSIIYDEAKGKAAG
                     VRVIDTHSKSVTEYTARIIFVNAACLNTNLILLNSRSRRFPQGIGNDNGLLGHYIAFH
                     NYRGSVHAEFEGFPDSYYYGRRPTEIFIPSFRNLRKHEGLGFQRGYMSYFSASRAGWW
                     QGEEKPGIGGEWKDAITEPGPWSVYMGMQGETIPKFDNHVRLSPDKKDEWGIPQLITS
                     VSYDDNDDRMAADFITQGSEMLEAAGCKNIGGGNNHQNPGL"
BASE COUNT          513 a          763 c          463 g          352 t
ORIGIN      
        1 ggtactcggc ctcgacgtct tcggcataaa aggccgtcca cgggatccct gcggcaaaca
       61 attcccgctg aaacactacc gcaggcggaa aacccagggg ctccaacacc acctccaccc
      121 catcgggttc ctcccccgaa acaaccgtaa gccatttatg ctcccccatc gggacctcct
      181 tcttcttcac aaaacccagc acctccgtat aaaaacgaag cgccttttcc tggtccttta
      241 ccaggacgct gaccagtctt actttcatgg taatcttttt agggcaaata tacccgcccc
      301 gccttatcgg aaattgcgca tttcgcccgg cgcacgaccc gtcatccgcc ggaaaagatg
      361 agagaaagaa ggcacgctgc taaacccgac ccgccagcaa acgctatgca cactcgcccc
      421 cgcacgaagc cactccttcg ctaaagcgat ccgccgctca gtcagatacc ggtgcggcgt
      481 ctgcccatag caacggctaa actgccggat aaaatgaaat ttcgatacaa agaccccccg
      541 ggcaattcat gatcaaatta cccatccccg acaattgctt cttttttact aaattactac
      601 actcaacgct tcccatatga cccaatacga cgccatcgtc atcggctccg gcatcagcgg
      661 cggctgggcc gcaaaagaac tctgcgaaca aggcctgaca accctcctgc tcgaacgcgg
      721 ccgccccgtg gaacacctca aggactatcc caccgccaca aaaaatccct gggactttcc
      781 ccaccgcggc caactccccc tgaaagtcct cgacgacaac cccatcgtgg cccgctgcta
      841 cgcctacggc gaagccaccc agcacttctt cgtaaaagac aaggaacatc cctatgtcca
      901 ggaaaaacct ttcgactgga tcaggggcta ccaggtaggc ggcaaatccc tgctctgggc
      961 ccgccagacc caacgctgga gccccttcga gttcgaaggc cccgcccgcg acggcatcgc
     1021 catcccctgg cccatcggct acaacgacct cgccccctgg tatagtcacg tagagaaatt
     1081 cgccggcatc agcggcaaca aagacggcct cccccaactg cccgacggag aattcctgcc
     1141 gccctgggaa atgaattgcg tagaacaaca cataaaggaa aagatcctgc aacgataccc
     1201 cgaccgcccg gtgatcatcg gccgctgcgc ccacctcaca aaaccccagc ccatccacct
     1261 cgaacagggc cgcggccaat gccaggcccg tagcctctgc gaacgcggct gccccttcgg
     1321 cggctacttc aactccaatt ccaccaccat cccctgggcc caacgcaccg gcaaactcac
     1381 gctccgcccc tacagcgtcg ttcactccat catctacgac gaagcaaaag gcaaggccgc
     1441 cggcgtccgc gtcatcgaca cccattcaaa atccgtcacc gaatataccg cccgcatcat
     1501 tttcgtaaac gccgcctgcc tcaacaccaa cctcatcctc ctcaactccc gatcccggcg
     1561 cttcccgcaa ggcatcggca acgacaacgg cctgctcggc cactatatcg ccttccacaa
     1621 ctatcggggt tccgtacacg ccgaattcga aggcttcccc gacagctatt actacggccg
     1681 ccgccccacc gaaatattta tcccctcctt ccgcaacctc cgcaaacacg aaggactcgg
     1741 ttttcaacgc gggtatatga gctatttctc ggcatcccgc gccggctggt ggcagggtga
     1801 agaaaaaccc ggcatcggcg gggaatggaa ggacgcgatc accgaacccg gcccctggtc
     1861 cgtctatatg ggcatgcagg gcgagacgat cccgaaattc gacaaccatg tccggctcag
     1921 ccccgataaa aaggacgaat ggggcatccc ccaactcatc acctccgtct cctacgacga
     1981 caacgatgac aggatggcag ccgatttcat cacccaggga tcggaaatgc tggaagcggc
     2041 cggctgtaag aacatcggcg gcggcaacaa ccaccagaac ccgggtctcg a
//
LOCUS       DATGFG010001106         4902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_407866, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001106 DATGFG010000000
VERSION     DATGFG010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407866"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /gene="ligD"
                     /locus_tag="VKQ52_20965"
     CDS             <1..1154
                     /gene="ligD"
                     /locus_tag="VKQ52_20965"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HLZ89743.1"
                     /translation="FRPDPPDAKATWLKPTLVCEVSFREMTVDGVMRHPSFEGMREDK
                     GAKEVVRETPVEINKVVKDAILKPVKKGERRTLLNPTDESQVRTINGHDLSFTHLSKV
                     FWPKEKYTKRDLLNYYYQVAPYILPYMKDRPQSLNRHPNGINGPSFYQKNVSGKVPDW
                     IETFPYHSEGDNEDKEFLVCTDEASLLYIISLGCIEVNPWSSRVKAPDNPDWCIIDLD
                     PDKKTPFDKVIEAAQVTKRVLDAAGIDSYCKTSGSTGIHIYFPLGAKYSYEQSKEFAR
                     VVVQRVNAEIPAFTTIERTVSARKGRMYLDFLQNRPQATIAAPYAVRPKPGATVSAPL
                     HWEEVKKGLSMQDFTIRTMGDRLKEMGDLFQPVLGKGIDMQKALKKLTK"
     gene            complement(1147..1575)
                     /locus_tag="VKQ52_20970"
     CDS             complement(1147..1575)
                     /locus_tag="VKQ52_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89744.1"
                     /translation="MKNDQYSAFFKALMTGLFIGIIDTLICLSYNIGYRSETGYIPSE
                     IINVSSLIFAVNLLLTIVGILYFFFHRFIRRGDVVYSIVFLLLTIWLASKTFSSVRFG
                     DARLDSGWHGLMGGIVLIVGISATCLPLLYRSEKFQDAVI"
     gene            complement(1582..3258)
                     /locus_tag="VKQ52_20975"
     CDS             complement(1582..3258)
                     /locus_tag="VKQ52_20975"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HLZ89745.1"
                     /translation="MDNSIPVSTSLGKLSPPNSSKRNSPVNRRVFYLSVQVIANAIVI
                     GVVAKALVYLIDLITNLSFYGRFSFEATSPAGNTLGWLVIIVPIIGAVLVGLMARFGS
                     KAIGGHGIPEAMEKIILADSRIPPAITFLKPLSAAISIGTGGPFGAEGPIIATGGAFG
                     SLTGQWMHISASERKIVLAAGACAGMAAIFGSPVAAVLLAVELLLFEFSPRSVIPVAL
                     ACVTGAGMHFLLFGHEPVFAMPSIPAASNGALAIYVLLGAFVGVVAAGVSRSIYWIED
                     RFEKLPIHWMWWPALGAIAIGVIGYFAPHTMGVGYDNIQHLLTGATPLKIAFSLCVLK
                     YLSWSISLGSGTSGGTLAPLFTIGGALGALLGTLILTWFPYAGINIPTAALIGMAAMF
                     AGASRAWLTSIVFALETTGQLNGLLPLLGGCVAAYFVSFFLMKGSIMTEKIQRRGVKT
                     PDAYEPDILRSITVRQLVIPVKPETVSKYAIAASEDAGLAAELMGKYNVDTLLVKDDK
                     DNEKVIGIVTSRSLLTFYSRQRQKEQHYQSPASTRRIMAQGRKLLRTNHK"
     gene            3343..4035
                     /locus_tag="VKQ52_20980"
     CDS             3343..4035
                     /locus_tag="VKQ52_20980"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLZ89746.1"
                     /translation="MNGISCDLTRCFLCRHSMPEWKELIALKKKTLFIKKGKTVFREG
                     EKVEGMFFLYSGAVKIHAPWVDGREMIVRFARGGEIVGHRGYGGGGPDSDVVYPVSAT
                     ALEDTTACFITNDFLEASLRANPSLTYTLMLFYATELQKAEKRMRDLTHMDVKGRIAN
                     ALLDIEAIFGRNGDGVIALALTRQDIAAYASTTYETVFKFFTELTAAGIICTAGKEIR
                     IQQADRLRVLAG"
     gene            complement(4024..>4902)
                     /locus_tag="VKQ52_20985"
     CDS             complement(4024..>4902)
                     /locus_tag="VKQ52_20985"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HLZ89747.1"
                     /translation="YKQQIHRVMLSRKQQQKYLAKRIGEWQTHLQEFTNSHNPETLHQ
                     LRVALKKLKALARFSKACSGSNAVKDANGLKKMFKQAGIIRDAGNHLHLLEQFHPVAE
                     PSSQPSSQPSSPSPSSAQPSSLPSSVSSSQQYKEQQEQVQTKATAKFIRHIKQYRRQG
                     KRAGKRLLADLDSIPVDCIRDWFALQLVTTGILLTSSGDRLHKARKQIKDLLYIQKLL
                     PAPAADVLHLDTEYLDKLQDAIGQWHDAAVIVAAWAGQDSEDSPAMTRKCREKEAAVR
                     HLAKDFYLRAHRTSPR"
BASE COUNT         1062 a         1127 c         1632 g         1081 t
ORIGIN      
        1 gtttccggcc cgatccgccg gatgcaaagg ccacgtggtt aaagccaacg cttgtctgcg
       61 aggtgtcgtt ccgggagatg acggtggatg gggtgatgcg gcatccctcg ttcgagggga
      121 tgcgggagga taaaggggcg aaggaggtgg tgcgcgagac gccggtggaa ataaataaag
      181 tggtgaaaga cgcgatccta aagccggtga agaaggggga acggaggacc ctgctcaatc
      241 cgacggatga gtcgcaggtg cggacgatca atgggcatga cctgagcttt acgcatctca
      301 gcaaggtgtt ctggccaaag gagaaatata ccaagcgcga tctgttgaat tactattacc
      361 aggtggcgcc ttatatcctg ccgtatatga aggacaggcc gcagtccctg aaccggcatc
      421 caaacggtat caacgggccc agcttttatc agaagaatgt gagcggcaag gtgccggact
      481 ggatcgagac ctttccctat cattccgagg gggacaatga ggacaaggaa tttctcgtct
      541 gtacggatga ggccagtcta ttatatatta tctcccttgg gtgtatcgag gtgaatccct
      601 ggagcagccg ggtgaaggcg ccggataatc ccgactggtg tatcatcgat ctcgatcccg
      661 acaagaagac gccttttgac aaggtgatcg aggccgcgca ggtgacgaag cgggtgctcg
      721 atgcggcggg gatcgattct tattgcaaga cctcgggatc gacggggatc catatctatt
      781 tcccgctggg agcgaagtac agttatgagc agtcgaagga atttgcccgg gtggtcgttc
      841 agcgggtgaa tgccgagatc cctgctttta ctacgatcga acgaacggta tcggcgcgca
      901 agggccggat gtacctggat tttttacaga accggccgca ggcgacgatc gcggcgcctt
      961 atgcggtgcg gcccaagccg ggggctacgg tatcggcgcc cctgcattgg gaggaggtga
     1021 agaaggggtt gagcatgcag gattttacca tcaggaccat gggcgaccgg ctgaaagaaa
     1081 tgggggatct ctttcagccg gtgcttggga agggtatcga tatgcaaaag gccctgaaaa
     1141 agcttactaa atgacggcgt cctggaattt ctcactccgg tagaggagag gcaggcaggt
     1201 agcgctgatg ccgacgatga ggacgatgcc gcccatcaat ccatgccagc cgctgtcgag
     1261 gcgtgcgtca ccgaaacgga cggagctgaa ggtcttcgag gcgagccaga tggtcagtag
     1321 caggaagacg atggagtaga cgacatctcc acggcggatg aagcgatgga aaaagaaata
     1381 aaggatgccg acgatggtaa gcagcagatt gacagcaaaa atcagcgaag acacattgat
     1441 gatttccgag gggatataac ccgtctcact gcggtagccg atgttatagg agaggcagat
     1501 gagggtgtcg atgatgccga tgaagaggcc ggtcatcagg gccttgaaga aggcggagta
     1561 ttgatcgttt ttcatacttt actatttatg atttgtgcgt aatagttttc ttccctgtgc
     1621 catgatccgc cgggtgcttg ccggggactg gtaatgttgt tctttttgcc gctgccggct
     1681 gtagaaggtg agcaggcttc gcgaggtgac gatgccgatc accttttcgt tgtccttgtc
     1741 atctttgacc aggagggtat cgacgttgta cttccccatg agttccgcgg cgaggccggc
     1801 gtcttcggag gcggcgatag catattttga aacagtttcc ggctttacgg ggatgaccag
     1861 ttgccggacg gttatgcttc tcaggatatc gggttcatag gcatccgggg tttttacgcc
     1921 gcggcgctgg atcttctcgg tcatgatgct tcccttcatc aggaagaagg agacgaagta
     1981 tgccgccacg cagccgccca gcaggggcag caggccattc agttgcccgg tggtttcgag
     2041 tgcgaatacg atggatgtaa gccaggcccg ggaggcgccg gcgaacatgg cggccatgcc
     2101 gatgagcgcc gccgtgggga tattgatgcc cgcgtaaggg aaccaggtga ggatcagggt
     2161 acccaggagg gcgccgagtg cgccgccgat ggtgaacagg ggggcgaggg taccacccga
     2221 ggttccgctg cccaggctga tcgaccagga caggtatttc aacacacaga gggaaaaggc
     2281 gatcttcaac ggggttgctc ctgtgaggag gtgttggata ttgtcatagc caacacccat
     2341 ggtgtgcgga gcgaaataac cgatgacgcc gatggcgatc gcgcccaggg cgggccacca
     2401 catccagtgg atgggcagct tttcgaacct gtcctcgatc cagtagatgg agcgggatac
     2461 gcctgcggcg acgaccccga cgaaggcgcc gagcaggaca tagatggcga gggcgccatt
     2521 cgaagctgcc gggatggagg gcatggcaaa aacgggctca tgaccgaaga gcaggaagtg
     2581 catacctgcg cccgtgacgc aggcgagggc cacggggatg accgaccgcg gggagaattc
     2641 gaataacagg agttctacgg ccagcaatac ggcggcgacc gggctgccga agatggccgc
     2701 cataccggcg caggctcccg cggcgaggac gatcttgcgc tcggaagcgg agatgtgcat
     2761 ccattgaccg gtgagcgagc caaaggcgcc gcctgttgcg atgatggggc cttcggcgcc
     2821 gaaggggccg ccggtgccga tcgagatagc cgcggacagg ggtttcagaa aggtgatcgc
     2881 cggtgggatg cggctatcgg ccaggatgat cttttccatt gcttcgggga tgccatggcc
     2941 tccgatggct ttcgagccaa agcgggccat caggccaact aatactgcgc cgatgatggg
     3001 gacgatgatg accagccagc cgagggtgtt gccggcgggg gaggtcgctt caaaggaaaa
     3061 gcggccgtaa aaggagagat tcgttatcag atcgatgaga taaacaaggg ctttggccac
     3121 gacaccgatg acgatagcgt tggcgatgac ctgaacgctc aggtagaaga cacgacggtt
     3181 tacaggggaa tttcttttgg atgaattggg ggggcttaac ttgccaaggg atgtagacac
     3241 gggtatactg ttgtccataa ttctgcaaat atattgtata aacaatatta tttggctatt
     3301 tttgatgttt aatttttaat aaagtattgt ataaacaata ttatgaatgg aatctcatgc
     3361 gacctgaccc gctgtttttt atgtcggcac agcatgccgg aatggaagga actgatcgcg
     3421 ctgaagaaaa agaccctttt tataaagaag gggaaaacgg tgtttcggga aggtgaaaag
     3481 gtggagggga tgttctttct ctattccggg gccgtaaaga tccatgcgcc ctgggtggat
     3541 gggcgggaga tgatcgtccg gtttgcccgg ggtggggaga tcgtggggca tcgggggtat
     3601 ggcggcggcg ggccggatag cgacgtggtc taccctgttt ccgccacggc gctggaggac
     3661 acgacggcct gttttatcac caatgatttt ctcgaggcga gtctcagggc caatccttcg
     3721 ctgacctata ccctgatgct tttttatgcc acggaattgc agaaggcgga gaagcggatg
     3781 cgcgatctga cgcatatgga cgtaaagggg cgcatcgcca atgcgctgct cgatatcgaa
     3841 gccatctttg gccgtaatgg ggatggcgtc attgcccttg cgcttacgcg gcaggatatc
     3901 gcggcttatg cgagcacgac gtatgagacg gtgttcaaat tctttaccga gctgaccgcg
     3961 gcggggatca tttgtaccgc gggaaaggag atccggatcc agcaggccga ccggctgcgg
     4021 gtgttagcgg ggtgaggtgc ggtgggcacg tagataaaag tctttggcga ggtggcggac
     4081 ggcggcttct ttttcccggc atttacgggt catggcgggg ctgtcttcgg agtcttgtcc
     4141 tgcccaggcg gcgacgatga cggcggcatc gtgccactgg ccgatggcgt cctgtagttt
     4201 gtcgaggtat tcggtatcga ggtgcagaac gtcggctgcc ggggccggca ggagtttttg
     4261 tatatacagc aggtccttta tctgtttccg cgctttgtga agccggtcgc ccgacgaggt
     4321 aaggaggatg cccgtggtta cgagttgcag ggcaaaccag tcacggatgc aatcgacggg
     4381 gatggaatcg aggtcggcga gcaggcgctt gccggcacgt tttccctggc ggcggtattg
     4441 ttttatatgt cggatgaatt ttgccgttgc ctttgtctgc acttgctcct gttgttcctt
     4501 gtattgttgt gatgatgaga ccgaggaggg gagcgaggat ggttgtgctg atgagggcga
     4561 aggcgaggag ggctgtgatg atggttgcga tgatggctct gctaccggat ggaattgttc
     4621 gagcaggtgg agatgattac ccgcatcgcg gatgatgccg gcctgtttga acattttttt
     4681 cagcccgttg gcgtctttta cggcgttgct gccggagcag gcttttgaga agcgggcgag
     4741 cgcttttagt tttttcaggg cgacgcgcag ttgatggagg gtttcggggt tatgcgaatt
     4801 agtaaactcc tgcaggtgtg tctgccattc cccaatcctt tttgcgagat atttttgctg
     4861 ttgctttctg gaaagcatca ccctgtgtat ttgctgttta ta
//
LOCUS       DATGFG010001107         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1550453, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001107 DATGFG010000000
VERSION     DATGFG010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550453"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..697
                     /locus_tag="VKQ52_20990"
     CDS             146..697
                     /locus_tag="VKQ52_20990"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ89748.1"
                     /translation="MRKRLLTAIIFLTVISSALAPITAGAQQKEPTLRSILLEQFRNT
                     WNKQDWFVPVAQSVEGLTAKQAMWKPADSSHSVGELAYHIMYWNREQLDKFYDRKPAP
                     FSGNNNETFTAFTEATWATTVEQLNKVMADWEKAIETADEAKLKKWASAIDHVNTHTA
                     YHTGQILYIRKLAGNWDPNKGVK"
     gene            complement(706..2406)
                     /locus_tag="VKQ52_20995"
     CDS             complement(706..2406)
                     /locus_tag="VKQ52_20995"
                     /inference="COORDINATES: protein motif:HMM:NF008183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HLZ89749.1"
                     /translation="MKRSLPFVSTLLLFVFCMNTSYGQWWKEAIVYQIYPRSFKDSDG
                     DGVGDLKGIISKLDYIKSLGVTAVWLNPIYSSPNDDNGYDVSDYRNIMKEFGTMSDFD
                     ALLKGLHARGIKLVMDLVVNHSSDEHEWFKQSRSSRTSPYRDYYHWWNAERGVPPYRY
                     SLFDVNHDAWRYDSLTNAYYLHYFSRKQPDLNWENPALRREVYNIMRFWADKGIDGFR
                     LDAFQFVAKDTTWPVWPKGWEEHFMKYYAMGPHLHDYLREMNGEVLSRYSVMSVAEGA
                     GNSFSDAHDLVDAHRKELNMAYAFDAVDIAKSSGYSLVHLKEVFSRWDSAFAENGWLA
                     VFLANHDQARLVSRFGNDRPAFRAVSSKMLSTFLLTMRGTPFYYNGDELGMSNPGFTK
                     IGDYRDVATLNEYRHLENTGGDLGAYLKEIAFGSRDNSRTPMQWDAGIHAGFTRGKPW
                     IGLNPNYSSVNVAAEDRDVNSCLNYFRKLTKLRKDNPVLVYGKYTLLDKGNPDVYAYT
                     REWQGVKWLVVLNFSTRTVTMPAGVSTEGANVMMDNYAVAGKEGELRAYEAIVYALPR
                     "
     gene            2626..>3700
                     /locus_tag="VKQ52_21000"
     CDS             2626..>3700
                     /locus_tag="VKQ52_21000"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ89750.1"
                     /translation="MRKLVAFLILSCLFSSLAMAQAPGQGAPGAKPGAPGARPGGPGG
                     APPAIGRVFGKVTDSTGQPIAATAVVLKSVTDPATKKKKLVLIKGMDTKANGEFNFED
                     LPIVQPLVVRLSATGYKNQDVSFTIVPATAQASPAPVGQGNSNPFGSMPSFEKDLGAV
                     KLATDITQLATATVTAKTPPMKLELDKKVFNVSQNIVSAGGTGVDVMRNVPSVNVDID
                     GNLTLRGSTPTIFIDGRPTTLTLDEIPADAIESVEVMTNPSAKYDASGGGAGIVNIVL
                     KKNRKQGYNGSISAGMDSHGQPNALVGLSLRQNKVNFTLNGMYNAMDPKTVGNTDRSS
                     NLNDTISRIQQSDLQRNKGHFMFG"
BASE COUNT          934 a         1164 c          827 g          775 t
ORIGIN      
        1 accctgtgac cataaagatt gttgaaagac cagcagcagg cagacccatg ccgccctgca
       61 caggaaggag gtgttaaaca taattgacgg ggttgatgtc ctataaagat agataaacta
      121 tatttgaaca aacaactgct aagccatgcg taaacgcctc ctcactgcca tcatcttcct
      181 cactgtcatc agtagcgcgc tcgcccctat caccgccggc gcccagcaaa aagaacccac
      241 cctgcggtcg atccttctcg aacagtttcg caatacctgg aacaaacagg actggttcgt
      301 tcccgtcgcc caatccgtgg aaggcctcac cgccaaacag gccatgtgga agccggccga
      361 ctccagccac tccgtcggtg aactggccta tcacatcatg tattggaacc gcgaacaact
      421 cgataaattc tacgaccgta aacccgcccc ctttagcggc aacaacaacg aaaccttcac
      481 cgccttcacg gaggccacct gggcgacgac ggtcgagcag ctgaacaagg tgatggccga
      541 ttgggaaaag gccatcgaaa cggcagacga agcaaaactg aaaaaatggg cctcggccat
      601 cgaccacgtc aacacccata cagcctacca tacggggcag atactttata tccgcaaact
      661 cgcgggcaac tgggatccga acaaaggagt aaaatagggc aatccctaac gcggtaacgc
      721 gtagactatc gcctcgtacg ccctcaactc cccttcctta cccgccaccg cataattatc
      781 catcatcacg ttcgcgccct ccgtcgaaac ccccgccggc atcgtcaccg tgcgcgttga
      841 aaaattcaac accaccagcc acttcacccc ctgccactcg cgcgtatacg catacacatc
      901 cgggttaccc ttgtccagca aggtatactt cccatacacc agcaccgggt tatccttcct
      961 caatttcgtc agcttccgga aataattcaa acagctgttc acatcccgat cctctgccgc
     1021 cacattcacc gacgaataat tgggattcag cccgatccat ggcttacccc gtgtaaaacc
     1081 cgcatggata cccgcatccc attgcatcgg tgtccgcgaa ttatcccgcg agccgaaggc
     1141 tatttctttc agataagccc ccagatcccc acccgtattc tccaggtgcc ggtattcgtt
     1201 caatgtcgcc acatcccggt aatcgccgat cttcgtaaaa cccgggttac tcatccccag
     1261 ctcatcgcca ttgtaataga acggcgtacc ccgcatcgtc agcaaaaagg tggacaacat
     1321 cttcgacgac accgcacgaa aggccggccg gtcattccca aaccggctca ccagccgcgc
     1381 ctggtcatgg ttggcaagaa agaccgccag ccagccgttc tccgcaaaag cgctgtccca
     1441 ccggctgaaa acctctttca aatgaacgag actataaccc gaagacttcg cgatatccac
     1501 cgcatcgaac gcatacgcca tattcaactc tttccggtga gcatccacaa ggtcatgcgc
     1561 gtcggaaaaa gaattacccg ctccctccgc aacactcatt acggaataac ggctaagcac
     1621 ttccccgttc atctcccgca gatagtcgtg cagatgcggc cccatcgcat agtatttcat
     1681 aaaatgctcc tcccagccct tcggccagac aggccatgtc gtatccttcg ctacaaactg
     1741 aaaggcatcc agccggaacc cgtcgatccc cttgtccgcc cagaaccgca taatattgta
     1801 tacctcccgg cgaagcgcgg gattctccca gttgagatcg ggctgcttac gcgaaaaata
     1861 atggagataa taggcattcg tcagcgaatc atatcgccag gcgtcgtggt tgacatcaaa
     1921 aagactataa cgatagggtg gcacaccccg ctccgcattc caccagtgat agtagtcacg
     1981 gtaaggactg gtccgcgaac tccggctttg tttgaaccac tcatgctcgt cgctgctgtg
     2041 attcacgaca agatccatca ccagcttgat cccccgcgca tgcagtccct tcagcagcgc
     2101 atcgaagtca ctcatcgtac caaattcctt cataatattc cggtaatcgc tcacgtcata
     2161 gccgttgtcg tcgtttggac tgctgtaaat ggggttaagc cataccgccg tcaccccaag
     2221 actcttgata taatccagct tcgagatgat ccctttcaga tcaccgacgc catcgccatc
     2281 actatcctta aagctccggg ggtagatctg gtaaacaatc gcctccttcc accattgacc
     2341 gtaactggta ttcatgcaaa aaacaaagag tagcagggtt gaaacgaatg gcaaggatct
     2401 tttcataaag ggctatttgt cggggtaact ttcaatttgt tacaaatata aaatattatt
     2461 tgttcgcgct ttatttgcaa aaaatgcacc cgttttatgc gcatgggcgc cgcttttcgt
     2521 ttgccgacag atttatgccg tttaccgcgt tttttcctcc gtttgcagta ttgctctcga
     2581 cggaaccaaa catcgaaatt acctttgtga tctaaaataa ctatcgtgag aaaattagtt
     2641 gctttcctta tcttatcctg cctcttcagc agcctggcga tggctcaggc tcccgggcaa
     2701 ggcgcccccg gcgcaaaacc gggcgcaccc ggtgcaagac ccggtggccc cggcggggct
     2761 cctcccgcca tcggtcgcgt cttcggaaag gtaacggatt cgacaggcca gcccatcgcg
     2821 gcgacggccg tcgtgttgaa gtcggtgacc gatcccgcaa caaaaaagaa aaagctcgtg
     2881 ctcataaagg gaatggacac aaaagccaat ggcgaattca acttcgaaga tctgccgatc
     2941 gtccagccgc ttgtcgttcg gctctccgcc accggttata agaaccagga cgtgagcttc
     3001 accatcgtcc ccgcaaccgc ccaggcatcc cccgcccccg tgggacaggg gaatagcaat
     3061 ccgtttggca gcatgccttc cttcgaaaag gatctcggcg ccgttaaact cgccaccgac
     3121 atcacgcaac tggcgacggc caccgttacc gccaagaccc cgccgatgaa actggagctc
     3181 gataaaaagg tcttcaacgt ttcgcagaat atcgtcagtg cgggcgggac aggcgtggat
     3241 gttatgcgca atgttccttc cgtcaacgtc gacatcgatg gtaatcttac ccttcgcggt
     3301 tcgactccaa ccatctttat cgacggccgc cccaccacgc ttacactcga tgagatcccc
     3361 gccgatgcta tcgaaagcgt cgaggtcatg acaaaccctt ccgccaaata tgacgcctcc
     3421 ggcggtggcg cgggtatcgt caatatcgtc ctgaagaaga accggaagca aggttataac
     3481 ggcagcatct ccgcgggcat ggactcccat ggacaaccga atgccctcgt cggcctcagc
     3541 cttcgtcaga acaaggtcaa cttcaccctc aatgggatgt acaacgcgat ggatcccaaa
     3601 accgttggca acaccgaccg gtcgtccaac ctcaatgaca cgatcagtcg tatccagcag
     3661 tcggacctgc aacgcaacaa ggggcatttt atgttcgggc
//
LOCUS       DATGFG010001108         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1550561, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001108 DATGFG010000000
VERSION     DATGFG010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550561"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VKQ52_21005"
     CDS             <1..526
                     /locus_tag="VKQ52_21005"
                     /inference="COORDINATES: protein motif:HMM:NF012527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89751.1"
                     /translation="SAYIPTNVISITDGQIFLEGNLFNSGIRPAINVGISVSRVGGNA
                     QIKSMKKVAGTLKLDQALYREMEAFSKFGGDLDAATKLIIDKGARNVEILKQPQYSPF
                     PVEKQVAIVYLGTQGLLRSVKVSKVREFEDHFLMEMENKLPDVLAEFKKGNLPDDGIK
                     KMVELANGLIPQYK"
     gene            1164..3437
                     /locus_tag="VKQ52_21010"
     CDS             1164..3437
                     /locus_tag="VKQ52_21010"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ89752.1"
                     /translation="MKAFALFMTLLLLAGWLPAQTRVSGTVKDGKGHPIHGASITIKD
                     SYDGATTDSLGNFHFQAGDKGNFTLITTNIGYNSVEQPVTLAGTPLELHIVMKEQLSE
                     LKAVTITAGSFTAGDAKRGAVLSSLDIATTAGSNADITAALKTLPGAQQVGEQEGLFV
                     RGGAGYEAKQYIDGTLVNNPYYTSVPDIAQRGRFSPFLFKGTVFSTGGYSALYGEALS
                     SVVLLESIDLPEKSEIDATISPLVVGLGTQQLAKNKQSSYGFSYDYVNAYAYFHAVPQ
                     DVDYFRMPQFHNADANFRIRTKGGGMIKYYTTFAYNDLGLRRQDWDSLNMKDAFGLTN
                     HNWYNNLSYREYLNNGWKMDLGASYSTNLDNLGQKVQNAMNQPQTFPDTVFWMNSKNF
                     TIDQRQDLSQVRAVFDKKLGTLSAIRFGSEYWYSYNYQKYRSPGYYYTIDTMYKFKDN
                     FNSLFAESDIYLTNALAAKVGLRYEHSTVINKSDLAPRVSLAYRTGPEAQVSLAYGIF
                     YQKPENSQLFASQNVGFTKATHYIINYQKMSKDRIFRVEAYYKKYEDLIKTVPLSNFN
                     TFFTTYNNSGSGYAKGIELFWRDKKSIKDFDYWISYSYLDTKRNYLNYTGQLTPNFAA
                     THTASIVMKRFFTKIKSGVNVTYSYATGRPYYNFILNGLKYNLAEQGKTKDYNSLNLS
                     AEYVPSIGNTKAKTFVVLFASVSNVLGYNSVYGYNFSYSGLYKQPITPPAKRFYFIGC
                     FLSWGVDRSQDAINNNL"
     gene            3473..>3986
                     /locus_tag="VKQ52_21015"
     CDS             3473..>3986
                     /locus_tag="VKQ52_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89753.1"
                     /translation="MRKTVLFLAMLVSLAASAQNDKYTQVMQKNISLLDSAKTIDELT
                     GLAATFDRIGDAEKNKWLPYYYAALAQTWIGWNPATKDKDANSAKINAYLAKAEALEK
                     NAELYAVENMSATQQMLVDPSTRWATNGKDANTALQKGLAADPNNPRLYYLQAMSLFG
                     TPPQFGGGKDK"
BASE COUNT         1006 a         1138 c          959 g          883 t
ORIGIN      
        1 gtcggcctac atcccgacaa acgtgatctc gatcaccgac ggacagatct tcctcgaagg
       61 caacctgttc aactccggta tccgtccggc gatcaacgta ggtatctccg ttagccgggt
      121 gggtggtaat gcgcagatca agtcgatgaa gaaagtggcc ggtacgctga agctggacca
      181 ggcgctttac cgcgagatgg aagccttctc caagttcggc ggcgacctcg atgcagcgac
      241 gaagctgatc atcgacaagg gcgcgcgtaa cgtggagatc ctcaagcagc cgcagtattc
      301 gcccttccct gttgaaaagc aggtggcgat cgtctatctg ggtacccagg gtctgctgcg
      361 gagcgttaag gtgagcaagg tgagggagtt cgaagaccat ttccttatgg agatggagaa
      421 caaactgccg gatgtactcg cagaattcaa gaagggcaat ctgcctgatg atgggatcaa
      481 gaaaatggtg gagctggcta acggtcttat cccgcagtat aagtaaaaac ttagtttgat
      541 aattcgcaaa acgctcccac ttgggagcgt ttttctttca aaaaaatagg tataaaggct
      601 tgcagaattc gaaacctttt acaatcttta cagcgtcgtc accgacaacc cctccaagtt
      661 cccgaaaaac aattcgatac ttttatttcc aatcctttat tgcagttcat ccgttcacga
      721 acgaaaaaat gaccgggctt ttttatcgcc gggggatccg taccaccatc gactcctctt
      781 ttgcggttaa tcccgtattc atctgattct catagtagcg acactcgtcc tcggccaatg
      841 gccaaaagga gaggatagct atccaaaata gtggcaacac caacttttta gttttaaagg
      901 gtacgatcaa caaccgccgg gcttttctaa gcctggcttt tttttatgac gccggaggcg
      961 tctttttgct cgtccagccg accgcagccg ccattcgcct cgctgtttcc caggacggtt
     1021 ccgccgctga gcgcccgtgt tcgccgaccg attgacgccg ttggccgccg attttccgcc
     1081 ggcaaccgag ttttttaaac aattttataa tttagtttgg tttataaaat aaaccagttt
     1141 accttagatg aaaattatga gaaatgaaag cttttgcctt attcatgacc ttactcctcc
     1201 tcgccggctg gcttccggcc caaaccaggg tctccggcac cgttaaagac ggcaaaggac
     1261 atcccatcca cggcgccagc atcaccatca aagactctta cgacggcgcc accaccgatt
     1321 ccctgggcaa tttccacttc caggccgggg ataaaggcaa ttttaccctt atcaccacca
     1381 atatcggcta taattccgtc gagcagcctg tcaccctcgc ggggacgccg ctggaactcc
     1441 atatagtaat gaaggaacag ctcagtgaac taaaggccgt cacgatcacc gccggctcct
     1501 ttacggcagg tgacgccaag cgcggggccg tcctcagctc cctggacatc gccaccactg
     1561 ccggctccaa cgccgacata acggcggcgc tcaagaccct ccccggcgcc cagcaggtgg
     1621 gggagcagga aggtcttttt gtccgcggcg gcgccgggta tgaggcaaaa caatatatcg
     1681 acggtaccct ggtgaacaat ccttattata ccagcgtacc cgatatcgcc cagcgcggcc
     1741 gtttttcgcc cttcctgttc aagggcaccg tcttcagcac cggcggctat tccgcgctgt
     1801 atggcgaggc gctttcctcc gtggtcttgc tcgaatccat cgatctgccc gagaaatcgg
     1861 agatcgatgc caccatctcc ccgctggtcg tgggcctggg cacccagcaa ctggcgaaga
     1921 acaaacaatc ttcttatgga ttcagctacg attatgtcaa tgcctatgcg tatttccatg
     1981 ccgtgcccca ggacgtagac tatttccgga tgccgcagtt ccacaacgcc gatgccaatt
     2041 tccgtatccg gaccaagggc ggcggaatga tcaaatacta caccacgttc gcttataacg
     2101 atttggggct ccgtcgccag gactgggaca gcctgaatat gaaggacgct ttcgggttga
     2161 ccaatcacaa ctggtacaat aacctgagct accgggaata cctcaacaac ggttggaaga
     2221 tggatcttgg cgccagctat agcaccaacc tggacaacct ggggcagaag gtgcagaacg
     2281 ccatgaacca gccacaaact ttcccggaca ccgtcttctg gatgaacagc aagaacttca
     2341 ctatcgacca gcgacaggat ctctcgcagg tgagggccgt cttcgacaag aagctgggga
     2401 cccttagcgc catccggttt ggctcggaat actggtattc ctacaactac cagaaatatc
     2461 gctcgcccgg ctattattat actatcgata cgatgtacaa gttcaaggac aacttcaatt
     2521 cgctgtttgc ggaatccgac atctatctga ccaatgcgct ggccgcgaag gtggggcttc
     2581 gttatgaaca ttccaccgtg atcaacaagt ccgatctcgc tccgcgggtg tcccttgctt
     2641 accggacagg tccggaagca caggtatccc tggcctatgg catcttctac caaaaaccgg
     2701 agaacagcca gttgttcgct tcacaaaatg tcgggtttac caaggctacc cactacatca
     2761 tcaattacca gaagatgtcc aaagaccgca tcttccgcgt ggaagcctat tataagaagt
     2821 atgaagacct gatcaagacc gtgccgttgt ccaatttcaa tactttcttt acgacttaca
     2881 acaacagcgg cagcgggtat gcgaaaggca tcgaactgtt ctggcgcgac aagaagtcga
     2941 tcaaggattt cgactactgg atcagctatt cctatctcga taccaagcgc aactacctga
     3001 actataccgg gcagcttacg ccaaatttcg cggccaccca cacggcctcc atcgtcatga
     3061 agcgcttctt caccaagatc aagtccggcg tcaacgtgac ctatagctat gccacgggtc
     3121 gcccctacta caactttatc ctcaacggcc tgaagtacaa tcttgccgaa cagggcaaga
     3181 caaaggacta caacagtctt aacctgagcg ccgaatacgt gcccagcatc ggcaatacca
     3241 aggcgaagac ctttgtggtg cttttcgcga gtgtgagcaa tgtattggga tacaattcag
     3301 tttacggata taatttctcg tatagcggat tgtacaaaca acccatcacc ccgccggcca
     3361 aacgcttcta ctttatcggc tgtttcctca gctggggggt cgaccgttcg caggacgcta
     3421 tcaacaacaa cttgtaaacc actgatcatt taatactata aaaactacaa ctatgcgtaa
     3481 aacagtcctt ttcctggcca tgctggtctc cctggcggca agtgcacaga atgacaagta
     3541 cacccaggta atgcaaaaga acatcagcct gctcgattct gccaagacga tcgacgaact
     3601 gacaggcctg gccgccactt tcgaccggat cggcgatgcg gagaaaaaca aatggctgcc
     3661 gtattactat gccgccctgg cccagacctg gatcggctgg aaccccgcca ccaaggacaa
     3721 ggatgccaac tccgccaaga tcaacgccta tctggccaag gccgaagccc tcgagaagaa
     3781 tgccgaactc tatgcagtgg agaatatgtc cgccacccag cagatgctgg tcgatccttc
     3841 cacgcgctgg gcaacaaacg gcaaggacgc caatacggct ttgcagaaag gactcgccgc
     3901 ggacccgaac aatccccggc tatactatct gcaagcgatg agtcttttcg gcactccgcc
     3961 gcaatttggt ggagggaagg ataagg
//
LOCUS       DATGFG010001109         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_697503, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001109 DATGFG010000000
VERSION     DATGFG010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697503"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VKQ52_21020"
     CDS             complement(<1..475)
                     /locus_tag="VKQ52_21020"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLZ89754.1"
                     /translation="MKHPNIFDWIRVDEYAATPKYLQLTNSILKAIENGQIRKNDLMP
                     SINELSYELEISRDTAEKGYKYLKKMGVLGSVPGKGYFIQNTDFHQTHRIFLLFNKLS
                     AHKKILYDALVASLGEYTVIDFYIYNNDFGLFKKLLTDRKEDYSHFVIIPHFLEGG"
     gene            complement(574..1638)
                     /locus_tag="VKQ52_21025"
     CDS             complement(574..1638)
                     /locus_tag="VKQ52_21025"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C1-like protein"
                     /protein_id="HLZ89755.1"
                     /translation="MKLILVATLALLLATNIDSGFDDLPINRVQVIGSHNSYKQPIDT
                     ALFSSFKAKNPQAAMGLEYSHVSLGEQLDLGLRNLEIDVYADEKGGKYAHPKGLEWEA
                     SANPPAYDPEGEMNKPGFKVFHVQDLDFRSNCPTFAGCLHQLKAWSDAHPDHYPIYIT
                     MNAKDAEIKAPGFTIPEKFTTGVFDHLDREILDNLGPAYLVTPDDIRGSYPSLEQAVL
                     ANHWPAMKTARGKFFFILDEGGEHRAAYIAGHPSLKGRVLFTDSPAGTPEAAFMIIND
                     AKRDLEKIKEMVKKGYMVRTRADSDTKEARANDRSSFTAACASGAQIITTDYYYKSKF
                     FPSDYIVSFADGSYIRKNQR"
     gene            1849..>2133
                     /locus_tag="VKQ52_21030"
     CDS             1849..>2133
                     /locus_tag="VKQ52_21030"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ89756.1"
                     /translation="MKLITRLFLPCFLFLSIAAQAFQQGPLPLKGVVTDENNIPLAGV
                     NVLVKGTQKAATTDAEGKYSIAVDGPGAVLQVSYVGFTPKEIRVGKEKDIN"
BASE COUNT          460 a          495 c          611 g          567 t
ORIGIN      
        1 ctccgccttc gaggaagtgc gggatgatga cgaagtgcga gtagtcctct tttctgtcgg
       61 tgagcagctt tttgaacaaa ccaaagtcgt tgttatagat gtagaagtcg atgacggtgt
      121 attcgcccag ggaggcgacc agggcgtcat agaggatctt tttgtgggcg ctgagtttgt
      181 tgaagagaag gaagatgcgg tgggtctggt ggaagtcggt attctggatg aaatagccct
      241 tcccggggac ggagcccagg acccccattt ttttcaggta tttatagcct ttttccgcgg
      301 tgtcgcggga gatctcgagc tcgtagctga gttcgttgat ggagggcatc aggtcgttct
      361 tcctgatctg gccgttctct atcgccttga ggatcgagtt ggttaactgc aggtattttg
      421 gggtggcggc gtattcatcc acccggatcc agtcgaaaat attggggtgc ttcatgcaga
      481 gcggtaatat aagaaaaaga gcccgataac cggaaaaatt tatcggcctc tttttcgcat
      541 catgtccggc aaatgaaatt tgccgggaac cagctatcgc tggttttttc ttatatagga
      601 accatcggcg aagctgacga tatagtcgga ggggaagaat ttgcttttgt aataataatc
      661 ggtggtgatg atctgggcgc cggatgcgca ggcggcggtg aaactggacc ggtcgttggc
      721 ccgggcttcc tttgtgtcgg agtcggcgcg ggtgcggacc atatagcctt ttttcaccat
      781 ctcttttatc ttctccaggt cgcgtttggc gtcgttgatg atcataaacg ccgcttcggg
      841 cgtgcctgcc ggcgaatcgg tgaacagtac ccggcctttt agcgagggat gaccggcgat
      901 gtacgcagcg cggtgttcac cgccttcatc caggataaag aagaatttgc ctcttgccgt
      961 cttcatggcc ggccagtggt ttgccaatac ggcctgctcc agcgaggggt agcttccccg
     1021 gatgtcgtcg ggtgtgacga ggtaggcggg gccgagattg tccaggatct ccctgtcgag
     1081 atgatcgaac accccggtag taaatttttc cgggatggtg aagccgggcg cttttatctc
     1141 cgcatctttt gcattcatcg tgatatagat gggatagtga tcgggatgcg cgtcggacca
     1201 ggccttcaac tgatgcagac agccggcgaa ggtggggcag ttgctccgga aatcgaggtc
     1261 ctgcacatgg aagaccttaa aaccgggttt gttcatttca ccttccggat cgtatgccgg
     1321 agggttggcg gaggcctccc attcgagtcc ttttggatgg gcatatttcc cacccttttc
     1381 atccgcatat acgtcgatct ccaggttgcg caaaccgaga tcgagttgct cgcccaggct
     1441 gacgtggctg tattccaatc ccatggcggc ctgcgggttc tttgccttga atgaggagaa
     1501 aagcgcggta tcgataggct gtttgtaact gttatgcgag ccgatgacct gcaccctgtt
     1561 gatggggaga tcgtcgaaac cgctgtcgat gtttgttgcg aggaggaggg cgagtgttgc
     1621 tacgagaatc agtttcatat tacaggttgt ttgtgcaaaa taaaggttgt ttatgaaaaa
     1681 acagccaaca ctttttttat ccaccgcgta gacctatgta ctacgctttt tattagcaat
     1741 tgtgaatccg cttaggagtc aataagataa tattccattt acaattgcat aacatgcctt
     1801 ttttctattt gccttccgca tgcttaacca aaccaaaaca ctttcatcat gaagttaatt
     1861 acaaggctct ttttaccatg ctttttattc ttgtccatcg ccgctcaggc ttttcagcag
     1921 gggcccctgc cgctgaaagg agtggtgacc gatgagaaca acattccatt ggccggggtc
     1981 aacgtcctgg tcaaaggcac acagaaagcc gcgacgaccg acgcagaggg gaagtattcc
     2041 atcgccgtgg acggtccggg cgcggtgctc caggtctcct atgtcgggtt cactccgaag
     2101 gagatcaggg tcggtaaaga aaaagacatc aac
//
LOCUS       DATGFG010001110        10508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1090408, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001110 DATGFG010000000
VERSION     DATGFG010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090408"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..679)
                     /locus_tag="VKQ52_21035"
     CDS             complement(35..679)
                     /locus_tag="VKQ52_21035"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLZ89757.1"
                     /translation="MIRILLILNGIAFFAQQAIPDLTQWGALHYWKSPLFKPHQVITH
                     MFLHGNFPHILFNMLALWMFGSILENYWGAKRFLNFYLICGIGAAIVQMLMIPFEARQ
                     AIGPHPEVSPEVLQQAIDQYVDIYSMIGASGAIMGVMAAFAYLFPNTEMYIFFVPMPI
                     KAKYVIPVYVLIDLFGGFSRAAGDNVGHFAHLGGAAVGLLIVMVSNRTNRRNFY"
     gene            complement(763..2172)
                     /gene="rlmD"
                     /locus_tag="VKQ52_21040"
     CDS             complement(763..2172)
                     /gene="rlmD"
                     /locus_tag="VKQ52_21040"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HLZ89758.1"
                     /translation="MRKRNKNIELSDILVEGYAAEGKSIARVDGKVIFIENAVPGDVV
                     NLRLGKNKKDWAEGFVTAFRQYSPQRVDPFCSHFGVCGGCRWQMLPYPLQLQYKQQQV
                     EDVLRRIGKVALPAITPILGAPETTHYRNKIEYTFSNRRFLLPRELNDPSVDPRQNVA
                     GFHAKGLFDKVVDIDTCHLQEEPTNRLRKAVRDFGLGRDLPFYDIKEHRGWLRNLQLR
                     ICTTGEIMANLVLGYEDEEQTRELAALLLQQFPELTTLLVTINTKWNDSIWDLEPRVV
                     HGKGYVIEQLEDFRFKIGPKSFFQTNTRQGERLYAVTRDYAELTGKETVYDLYCGTGS
                     IGIFVSRQAARIVGVEVVPEAVSDARENAALNNIPHARFFAGDVVDICTDEFFSREGR
                     PDVIITDPPRAGMHEGLVKKILEMAAPTVVYVSCNPATQARDLHLLDAKYAVTRVQPV
                     DMFPHTHHIENIVQLKLRG"
     gene            2266..3663
                     /locus_tag="VKQ52_21045"
     CDS             2266..3663
                     /locus_tag="VKQ52_21045"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HLZ89759.1"
                     /translation="MKAYPQGGYSIHLHLKPFTGGKVYLGYYYGKIRALADSAQLDAN
                     SDGVFAGKERLPGGVYFIVSPGRAVLFELLLDSLQHFSVSADTASLPGSVVFTGSPDN
                     TVFQTYTRYTATKGKEITAAQKELAAAHGKADSARLLEKLKRANTEIQDYRQTLAGKY
                     PNTLLATLFRLLKEPVVPPASGQPGGRYDSLFAYHFFKEHYWDGVSFSDERLSRTPVF
                     EPRLDKYFRDLVPPEADSIEREADRMLLEARVSKAMFQYLLVYFVQKYVNPEYMGQDA
                     VFVHLFERYINTGQAEFFTEKYRKFLDNRAYSLMANLIGEPAANLEMVDSAGTQRNLY
                     DVTAPFVVICFWDPTCSHCKEIVPRVDSIFHAKWKQEGVKLYGVMVDGGREAWLQFIK
                     DHNLTDWIHVYETREHQDAGEKAGQPNYRQLYDVYQTPILYLLDKDKHIVAKKLSYQQ
                     IDEVINLKMRQKKSN"
     gene            3665..5608
                     /locus_tag="VKQ52_21050"
     CDS             3665..5608
                     /locus_tag="VKQ52_21050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLZ89760.1"
                     /translation="MSITVKTLFLIPALFLFFLSGLSAQTLFTIDHNPVGKEEFLKAY
                     NKNNTGQKPTEKSYRDYLELYIRYKLKVRAAYDAELDTLPGQRMELQNFRSQVAETYL
                     KDELSLDRLIREVFHRGQKDLHLSHILITLPKNPAPADTLRAYEKAMTAYAALKKGKK
                     FGEVAIQYSDDPGAKGNAGSLGYITVFTLPYQLETLAYSLAPGQFSRPYRTGGGYHIF
                     RNDGERKAIGRIKAAQILLAFPPSATETMKVAIKQKADSIYAQLQQGGDFAALARANS
                     GDNLSYQQGGELPEFGVGRYDSTFEATAFALDGDGAISKPLLSAYGYHIIKRLGRKSF
                     PTQLDEPAITQLKQQVLNDPRIEVSRKALLDRILQQAEFRRADYSDLDLWAFTDSTLR
                     NMGLTNFRGLSYSTVLFSFARQSYTLKGWLDYARTARGQRLGDPQSDKAVFDHYIERS
                     AQDYYRNHLEEYNKDFAFQLMEFKEGNLLFEIMQRKIWDKASTDSAGLRKYYDAHKEK
                     YWWEASADALLFTCNNERTAQDLKARLQVHPVGMWRQMADSGASAVQADSGRYELTQI
                     PNPTKADLSPGSYTPFSTNAADNSVSFAYIQNVYRDRAQRNFRDARGFVINDYQTYLE
                     DQWIAELKKKYAVKVDEAVLKGL"
     gene            complement(5627..6580)
                     /locus_tag="VKQ52_21055"
     CDS             complement(5627..6580)
                     /locus_tag="VKQ52_21055"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HLZ89761.1"
                     /translation="MKVIDHIRNGKDTLVSFEILPPLKGKTISSIYEHLDPLMEFKPS
                     FINVTYHRSETMFKKKTDGTFEKVEVRKRPGTVGICAAIMNHYKVDAVPHLICGGFAK
                     RETEDALIDLHFLGIDNVLVLRGDAAKNESSFEPEPDGHRYAIDLLKQVACLNNGVYL
                     EEDIKNGGKTNFCIGIAGYPEKHFEAPNLETDLMYLKAKVDAGAEYITTQMFFDNQKY
                     FEFVKTLRDRDITIPVIPGLKPITTKKQLSMLPKVFHVDLPTDLSSAILQCKTDAEVE
                     QVGTEWLIQQSRELKKAGVPVLHYYTLGKPKVIEAVVKATL"
     gene            6785..7567
                     /gene="lptB"
                     /locus_tag="VKQ52_21060"
     CDS             6785..7567
                     /gene="lptB"
                     /locus_tag="VKQ52_21060"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HLZ89762.1"
                     /translation="MSITIHSQDLVKTYHNRTVVNHVSVKLEQGEIVGLLGPNGAGKT
                     TTFYMVVGLIKPDEGDVFLNDVNITRQPMYKRAQMGIGYLPQEASVFRKLSVEDNIRA
                     VLEMTKLSRAQQKEKLEALLDEFHLHHVRKNNGDSLSGGERRRTEIARALAVDPKFIL
                     LDEPFAGVDPIAVEDIQSVVARLKFRNIGILITDHNVNETLSICDRAYLLIDGKIFKH
                     GTAEELAEDEQVRRLYLGRNFELKRKDYLHEEARQQEEKNTP"
     gene            7631..9163
                     /locus_tag="VKQ52_21065"
     CDS             7631..9163
                     /locus_tag="VKQ52_21065"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HLZ89763.1"
                     /translation="MKLLSPAISRLARLRQERIEQWMKHPVAVQREVLQHLVTSAQYT
                     EIGRKYNFSRIFSLREFKQAVPIHEYDDLKPYIQRIMNGEQNILWNTPINWFAKSSGT
                     TSDKSKFIPVSEESLQDCHYKGAKDVLTMYYNFNPESDLLTGKGLVIGGSHTINQVNE
                     DVHYGDLSAVLLQNSPFWGHWIRTPELSIALLDEWEDKIERLARNTIIENVTSISGVP
                     TWTMVLIRRILEITGKSTLAEVWPSLELYIHGGVAFTPYREQFRRLIGKDIYYLDMYN
                     ASEGFFAAQGNPHDDGMLLFLDHGIFMEFMPVEEYGKAHPETIGLPDVEIGHNYALVI
                     NTNGGLWRYLLGDTIQFTSRDPFRIKVSGRLKHFINAFGEEVIVDNSDKAIAIAAQKT
                     GAIVNDYTAAPVYFGETGNGAHEWLVEFEKQPDDLAQFVTELDKALQSINSDYEAKRH
                     KDIALGMPRLHVLEKGVFTSWLKRKGKLGGQHKVPRLSNDRKYIEEIQAMLSSGTAAS
                     AQ"
     gene            complement(9126..10379)
                     /locus_tag="VKQ52_21070"
     CDS             complement(9126..10379)
                     /locus_tag="VKQ52_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89764.1"
                     /translation="MNHPKNLPVKLLLLFTVETLSVTWCLRIQGWIPFFSLLYFASGI
                     AIAVLLTRCPALQLPAPGKRAWALPLNHYRLVLVGLMTLATWSWCVYWFEEMPIDITN
                     ADMLPIIKVMGERFIAGQHSHIYDTIPWIWHGTQPIYLPAMWLPYVPAIFIGIDMRWI
                     AVAGLLFAFTVFIFLYRPIPSSGETRADSRTRGYAPFFLGVVAFLLFWWLVADNTPGI
                     VSVAEEGMVIGYYTLLVLALVSGKPWLTGIAISLCMLSRYALVGWIPAYILCLALEKK
                     WRNLSIITLTGMLCFVLLFLLPVGLNTFVRLAKLPADYVAFAGRVWKDSPDTFSTAPG
                     LAWFFGPKRVGILHRLLVILSFLAPPLFILAGHRLKGANLPLAALKLALVIFFCFIDV
                     PYLYLFYTSSFVSLIALTLLFRTTA"
BASE COUNT         2414 a         2667 c         3115 g         2312 t
ORIGIN      
        1 gaaaacgcgg ataaacaaag gatgttcgcg ccggctagta aaaattccgc cggttggtcc
       61 ggttcgagac catgacgatg agcaggccca ccgccgcgcc cccaagatgc gcaaaatgcc
      121 cgacattatc cccggccgca cggctaaagc cgccgaagag gtcgatcagg acgtatacgg
      181 ggatcacgta cttggccttg atgggcatcg gcacgaagaa aatatacatt tccgtattgg
      241 ggaacaggta ggcgaaggcg gccatgacgc ccatgatggc gccggaggcg ccgatcatgg
      301 agtatatatc gacatattga tcgatggcct gttgcaagac ctccggcgat acttcaggat
      361 gtggaccaat cgcctgtctg gcctcaaagg ggatcatgag catctggacg atagccgcgc
      421 cgatgccgca gatgagatag aaattcagga agcgtttggc tccccagtaa ttttccagga
      481 tgctgccgaa catccaaagg gcaagcatat tgaagaggat atgcgggaag ttgccatgaa
      541 ggaacatgtg ggtgatcacc tggtggggtt tgaacagggg tgatttccag tagtgcaacg
      601 ccccccattg ggtcagatcg ggtatcgcct gttgggcgaa aaaagctata ccgttgagaa
      661 tgagtaatat cctgatcact gtcggaaaac tcggcgaacc tgttctaaag ctgggcatat
      721 tttttcttgt tctattaaat tctattagcg cacaatttac gattaacccc tcaatttcaa
      781 ttgcacgata ttttcaatat gatgggtatg cgggaacata tccaccggtt gaacgcgggt
      841 gacggcatat tttgcatcca gcaggtgaag atcccttgcc tgggtggccg gattgcagct
      901 tacgtagacc acggtgggcg ccgccatttc caggatcttt ttcaccagtc cttcgtgcat
      961 accggcgcgc ggcgggtcgg taatgatcac atcgggccgg ccttcccggg aaaagaactc
     1021 atccgtacaa atgtcgacga catccccggc aaagaaccgg gcatgcggga tgttgttgag
     1081 cgccgcattt tcgcgggcgt cggagacggc ttcggggacg acttccacac ccacgatccg
     1141 ggcggcctgg cggctgacga agatgccgat gctgccggtg ccgcagtaca ggtcgtagac
     1201 cgtttctttc cccgtcagtt ccgcgtagtc gcgtgtcacc gcgtagagcc gttcgccctg
     1261 gcgggtgttt gtctggaaaa aggatttggg accgatcttg aagcggaagt cttccagctg
     1321 ttcgatcacg tatcccttcc catggacgac ccggggttcc aggtcccaga tgctatcgtt
     1381 ccacttggtg ttgatcgtca ccaaaagagt ggtgagttcg gggaattgtt gcagcaggag
     1441 ggcggccagt tcccgggttt gttcttcgtc ctcgtatccc agcacgagat tggccatgat
     1501 ctctcctgtc gtgcagatgc ggagttgcag gttgcgcagc cagccgcggt gttccttgat
     1561 gtcatagaag ggcaggtcgc gcccgaggcc aaaatccctg acggccttac ggaggcggtt
     1621 ggtcggttct tcctgcaggt gacaggtatc gatgtctacc actttgtcga agaggccttt
     1681 tgcgtgaaag ccggcgacgt tctgccgggg gtcgacggat ggatcgttca gttcccgcgg
     1741 cagcaggaag cggcggttgg agaaagtgta ttcgatcttg ttccggtagt gggtggtttc
     1801 cggggcgccg aggatcgggg ttatcgcagg gagcgctact ttcccgatgc gccgcaggac
     1861 atcttccacc tgttgctgtt tatactgcaa ttgcagcgga tagggcagca tctgccagcg
     1921 gcagccgccg cagacgccga agtgggagca aaaagggtcc acgcgttggg gggagtattg
     1981 gcgaaaggcg gtgacaaagc cttcggccca gtcttttttg ttcttgccca gtctgaggtt
     2041 gacgacatcc cccggtacgg cattctcgat aaaaatgacc tttccatcca cgcgggcgat
     2101 ggacttccct tccgccgcgt atccttctac caggatatcg gataactcaa tgtttttatt
     2161 tctttttctc acggtgtaaa agtagtgggt ttttcccgcg actagtgcta tttttacctg
     2221 tgtatgagga agatcctttt atccgtgggc acattgttgt gcctcttgaa agcgtaccca
     2281 cagggcggat actccatcca cctccacctg aagcctttta ccggcgggaa ggtttacctg
     2341 ggttattatt atgggaagat cagggccctt gcggattccg cgcagctgga cgccaacagt
     2401 gacggcgttt ttgcgggcaa ggaacggttg cccggagggg tttattttat cgtttccccg
     2461 ggcagggcgg tgcttttcga gctgttgctg gacagcctgc aacatttctc ggtgtcggcc
     2521 gatacggcca gtctgcccgg cagtgtcgtg ttcaccggtt cgccggacaa tacggtcttc
     2581 cagacctata cccgttatac ggccacgaag ggtaaggaga ttaccgcggc gcaaaaggaa
     2641 ctggcggctg cgcatggcaa ggcggattcg gcccggttgc tggaaaaact gaagagggcc
     2701 aatacggaga tccaggatta ccggcagacc cttgccggca aatatcccaa tacgctttta
     2761 gcgacccttt tccggctgct gaaggaaccg gttgtgccgc ccgcctccgg ccagccgggt
     2821 ggaaggtatg acagcctttt cgcctatcat tttttcaagg agcattactg ggacggggtc
     2881 tctttctcgg atgagcggct tagccggacg cccgtgttcg agccccggct ggacaaatat
     2941 ttccgcgacc tggtgccccc ggaggcggat tccatcgagc gggaggcgga ccggatgctg
     3001 ctcgaggccc gggtcagcaa ggccatgttc caatacctcc tcgtctattt tgtgcagaaa
     3061 tacgtcaatc ccgagtacat gggacaggat gcggtgtttg ttcacctctt cgaacgatac
     3121 atcaataccg gccaggcgga gttctttacg gaaaagtacc ggaagtttct tgacaaccgg
     3181 gcttacagcc tgatggccaa cctgataggc gagccggcgg ccaacctgga aatggtggac
     3241 agcgcgggga cgcagcgaaa cctctatgat gtgaccgcgc ccttcgtcgt catttgcttc
     3301 tgggacccca cctgcagtca ttgcaaggag atcgtgccga gggtggactc cattttccac
     3361 gccaagtgga aacaggaagg cgtaaagctc tatggggtga tggtggacgg agggcgggag
     3421 gcctggttgc aattcattaa ggatcacaat ttaaccgatt ggatacacgt ttatgagacc
     3481 agggaacacc aggacgccgg cgagaaggcg gggcaaccca attaccgcca gctgtatgac
     3541 gtgtaccaga cccccatcct ttatttactg gataaagaca aacatatcgt cgccaaaaag
     3601 ctatcgtatc aacagatcga tgaggtcata aacctgaaaa tgcgtcaaaa aaaatccaat
     3661 taagatgagc atcacggtta aaacactctt ccttatccct gcccttttcc tgttctttct
     3721 ttccggtctg tccgcgcaga cgctcttcac gatcgatcat aaccctgtgg gcaaggagga
     3781 attcctgaag gcctacaata agaacaacac cgggcaaaaa cctaccgaga aatcctatcg
     3841 cgactacctg gaattatata tccgctataa attaaaagta cgggccgcct atgatgccga
     3901 gctggacacc cttccggggc agcggatgga attacagaac ttccgctcgc aggtggcgga
     3961 gacgtatctg aaagacgaac tgagtcttga ccggcttatc agggaagtct ttcatcgggg
     4021 tcagaaagat ctccatctgt cgcatattct tatcacgctg ccgaagaacc cggctccggc
     4081 cgatacgctc cgggcttatg aaaaggcgat gacggcctat gcggcgttga aaaagggcaa
     4141 aaaattcggg gaggtggcca tacaatattc ggatgatccc ggggcaaagg gcaatgccgg
     4201 gagtcttggt tatatcacgg tattcacgct tccctatcaa ttggaaaccc tggcctattc
     4261 gcttgctccc gggcagttct cccggcctta ccggaccggc ggcggatatc atatcttccg
     4321 gaatgatggg gagcggaagg ccatcggccg gataaaggct gcccagatcc tgctggcctt
     4381 cccgccatcc gcgacggaga cgatgaaggt ggcgatcaaa cagaaagccg actcgatcta
     4441 tgcgcagttg cagcagggcg gggattttgc cgcgctggcc cgcgccaaca gcggggacaa
     4501 tctgtcctat cagcaggggg gtgagttacc cgaattcggc gtcggccggt atgattccac
     4561 ctttgaagcg acggcctttg cgcttgacgg cgatggcgcc atcagcaagc ccctgctcag
     4621 cgcgtatggt tatcatatca tcaaacgtct tggccgtaaa tctttcccca cgcagctgga
     4681 tgaaccggcg atcacccagc tgaagcagca ggtgttgaac gatccccgga tcgaggtatc
     4741 gcgcaaggcc ctgctcgacc ggatcctgca gcaggccgag ttccgccggg cggactattc
     4801 cgatctggat ctctgggcct ttacggacag cactttgcgc aatatggggc ttaccaactt
     4861 cagggggctt agctattcca ccgttctatt ctcctttgcg cgtcaatcct ataccctgaa
     4921 ggggtggctg gactatgccc gaacggcaag ggggcagcgg ctgggggatc cccagtcgga
     4981 taaggccgtg ttcgatcatt atatcgagcg gagcgcccag gactattatc gcaaccatct
     5041 cgaagaatac aacaaggact ttgcttttca gctgatggaa ttcaaggagg gcaacctgtt
     5101 gttcgagatc atgcaacgga agatctggga taaggcgtcc accgacagcg cgggattaag
     5161 aaaatactat gatgcccata aggagaaata ctggtgggag gcgagtgcgg atgcgctgtt
     5221 gttcacctgc aataacgaac ggacggcgca ggacctgaag gcgcggttac aggtgcaccc
     5281 ggtggggatg tggaggcaaa tggccgatag cggcgcatcg gcggtgcagg ctgattccgg
     5341 tcgctacgag ctgacccaga taccgaatcc gacgaaggcg gacctgagcc cgggatcgta
     5401 cacgcccttc tcgacgaatg cggcggacaa ttcggtgagt tttgcatata tccagaatgt
     5461 ctatcgcgac cgggcccagc gcaatttccg ggatgcgaga ggatttgtga tcaacgacta
     5521 ccaaacctac ctggaagatc agtggatcgc cgagctgaaa aagaaatacg ctgtaaaagt
     5581 ggatgaggcg gtgttgaagg gcctttagtc ttttattcaa acccctttac aaggtggcct
     5641 tgacgacggc ttcgatcacc tttggcttac ccagggtata atagtgcagg acgggcaccc
     5701 cggccttttt caattccctg ctttgctgga tcagccattc ggtgccgacc tgttccacct
     5761 ccgcatcggt cttgcattgc aggatcgcgc tggagagatc ggtggggagg tcgacatgga
     5821 acaccttcgg cagcatgcta agctgtttct tggttgttat cggctttagc cccgggatga
     5881 cggggatagt gatgtcgcgg tcgcgaaggg tctttacaaa ttcaaaatat ttctggttgt
     5941 cgaagaacat ctgggtggtg atgtactccg cgccggcgtc gacctttgct ttcaggtaca
     6001 tcaggtcggt ttcgaggttg ggggcctcga aatgtttttc cgggtagcct gcgatcccga
     6061 tgcagaaatt ggtctttccg ccattcttga tatcctcttc gagatagaca ccgttgttga
     6121 ggcaggccac ctgtttcagg aggtcgatgg catagcgatg cccatcgggt tcgggttcga
     6181 aggagctttc gttctttgcc gcatcgcccc gcaggacgag cacattgtcg atgccgagga
     6241 aatgcagatc gatgagggcg tcttccgttt cccgtttggc gaagccgccg cagatgagat
     6301 ggggaacggc atccacctta tagtggttca tgatggcggc gcagatgccg accgtgccgg
     6361 gacgtttgcg gacttcgacc ttctcgaatg tcccgtcggt ctttttcttg aacatcgttt
     6421 cgctgcgatg ataggtaacg ttgataaagg agggtttgaa ctccatcagg ggatcgaggt
     6481 gttcgtatat agagctgatg gtcttgcctt ttaggggagg gaggatctcg aaggaaacaa
     6541 gtgtgtcttt tccgttcctg atatggtcta taaccttcat atgccgtatt gctacatttt
     6601 aagtgtgcaa acttaacgga ttacggcaaa aaataagcta aatataattt tttgccgcaa
     6661 ccgtgacaat gtcgggaccg gattccatcc ggtgggcgat aagtcttaaa acacctataa
     6721 atttaaggcc cggagcttgc ggcatggctt tcggggctta tttttgcggg caaaacagca
     6781 aaacttgagc attaccattc attcccaaga ccttgtaaag acctaccaca acaggaccgt
     6841 ggtgaatcat gtgtccgtga agctggagca gggggagatc gtcgggttgc tgggacccaa
     6901 tggcgccggc aagacgacga ccttttacat ggtggtcggg ctgatcaagc cggatgaagg
     6961 ggatgttttt ctcaatgacg tcaatattac ccgtcagcct atgtacaagc gggcgcagat
     7021 ggggatcggc tatctgccgc aggaggcttc cgtcttccgg aagctgagtg tagaggacaa
     7081 tatcagggcc gtgttggaga tgaccaagct cagccgggcg cagcaaaagg agaagctgga
     7141 agcgttgctg gacgagttcc acctgcatca tgtgcggaag aacaatgggg attcgctcag
     7201 tggtggggaa cggcggcgta ccgagatcgc cagggccctt gccgtggacc ccaaattcat
     7261 cctgttggac gaaccctttg cgggggtcga tccgattgcg gtggaagaca tccaatccgt
     7321 ggtagcccgg ctcaaattca ggaatatcgg tatcctgatc accgatcata atgtaaacga
     7381 gacgctttcc atctgcgacc gggcctacct gctcatcgac gggaagatct tcaagcatgg
     7441 aacagccgag gagctggctg aggacgagca ggtaagacgg ctgtacctgg gccggaattt
     7501 cgagttgaaa agaaaggatt atctccacga agaagcccgg cagcaggaag aaaaaaatac
     7561 gccttaaatg tcccggcgat taattacatt gccatagatg agaggagcgg ctcctccgtc
     7621 ggcttaatgt atgaaactac tgagtccggc catatcgaga ttggcgaggt tgcgccagga
     7681 acgcatcgaa caatggatga agcatcctgt ggccgttcag cgggaagtat tgcaacatct
     7741 tgtcacctca gcgcagtata cggagattgg ccgcaaatac aatttctcca ggatcttttc
     7801 cctccgtgaa ttcaaacagg cggttcccat ccacgaatat gacgatctga agccctatat
     7861 ccagcggatc atgaacgggg agcagaatat cctctggaat acgccgatca actggtttgc
     7921 caagagcagc gggaccacca gcgacaagag caagttcatc cccgtcagtg aggagagtct
     7981 gcaggactgt cattacaagg gtgccaagga cgtcctgacg atgtattata atttcaatcc
     8041 cgaatccgac ttgctgaccg gcaaggggct tgtcatcggc ggcagccata ccatcaacca
     8101 ggtgaacgag gatgtgcact atggcgacct gagtgcggtg ttgttgcaga attcgccttt
     8161 ctgggggcat tggatccgga cgccggaatt gagtatcgcc ctgctcgatg agtgggagga
     8221 taagatcgaa cgcctggccc ggaatacgat catcgagaat gtcacttcta tttccggtgt
     8281 ccctacctgg acgatggtgc tgatccgccg gatcctcgag atcacgggga agagcaccct
     8341 tgccgaggtg tggccttccc tcgaattata tatccatggc ggggtggcct ttactccgta
     8401 ccgggagcag tttcgccgcc tgatcgggaa agacatttac tatctcgata tgtataatgc
     8461 cagtgagggg ttctttgccg cccagggcaa tccgcatgac gacgggatgc tgctctttct
     8521 cgatcatggc atctttatgg agttcatgcc ggtggaggaa tacggtaagg cgcatccgga
     8581 gaccatcggg cttcccgatg tggagatcgg gcacaactat gcgcttgtta tcaacacgaa
     8641 tgggggcctt tggcgttatt tgctgggaga taccattcaa tttacttccc gggacccctt
     8701 ccggatcaag gtgagcggcc ggttaaagca ttttatcaat gcttttggcg aggaagtgat
     8761 cgtcgacaat tcggataagg ccattgccat cgccgcgcag aagacggggg ctatcgtcaa
     8821 tgactatact gccgcgccgg tctatttcgg ggaaacgggc aacggggcgc atgagtggct
     8881 ggtcgagttc gagaaacagc cggatgacct ggcgcagttt gtcaccgaac tggataaggc
     8941 gttgcaaagc atcaacagtg attatgaggc caagcggcat aaggatatcg cccttgggat
     9001 gccgcggctc catgtcctgg aaaaaggggt ctttacttcc tggctcaagc gaaagggtaa
     9061 gcttggcggg cagcataagg ttccccggct ttccaatgac cggaagtata tcgaggagat
     9121 ccaggctatg ctgtcgtccg gaacagcagc gtcagcgcaa tgaggctgac gaaggaagag
     9181 gtatagaaca ggtacagata ggggacatcg ataaaacaga aaaagatcac cagcgccagc
     9241 tttagtgccg caagcggcag gttggcccct ttcagccggt ggccggcgag tatgaagagc
     9301 ggcggcgcga gaaaggagag tatgacgagc agccggtgca atattcccac tctttttgga
     9361 ccaaagaacc aggcaagacc gggcgcagtc gagaaggtgt ccgggctgtc cttccacacc
     9421 cgtcctgcga aggccacgta gtccgcgggc agtttcgcca ggcggacaaa ggtattcagg
     9481 cccaccggca acaggaacag caatacaaaa cacagcatcc ctgtaagggt tatgatcgaa
     9541 aggttgcgcc atttcttttc cagggcgagg cagaggatat aggccggtat ccagcctacg
     9601 agggcatagc ggctcagcat gcatagggat atggcgatac ccgttagcca gggtttgccg
     9661 gagaccagcg ccaggaccaa cagggtataa tacccgatga ccatcccttc ttcggccacg
     9721 ctgacgatac ccggggtgtt gtcggctacc agccaccaga acaggaggaa ggcgacgacg
     9781 ccaagaaaga aaggggcata cccccgggtg cgggaatcgg cccgggtctc gccggaggag
     9841 gggatgggcc ggtaaaggaa gatgaagacc gtaaaagcga agagcaggcc cgccacggcg
     9901 atccagcgca tatcgatacc gatgaagatg gcgggcacat agggcagcca catcgccggc
     9961 aggtagatcg gttgggtgcc gtgccagatc caggggatgg tgtcatagat atggctgtgt
    10021 tgaccggcga taaaccgttc gcccattact tttatgatgg gcagcatatc ggcgttggtg
    10081 atatcgatgg gcatttcctc gaaccagtag acgcaccagg accaggtggc gagtgtcatc
    10141 agccccacga ggaccagcct gtaatggttg agtggaaggg cccaggccct tttaccggga
    10201 gcgggcagtt gcaaggcggg gcagcgcgtc aggagaacgg cgatggcgat gccggaggca
    10261 aaatagagga gggaaaagaa gggtatccag ccctggatcc tgaggcacca ggtgacgctg
    10321 agggtttcaa cggtaaagag cagcaggagt tttaccggca aatttttcgg atgattcatc
    10381 gatcctaaag atatatattt ggcccatgaa attactcgaa aacaaagtag cgatcgtcac
    10441 cggcgccagc cgtggcatcg gtgaggccgt ggctctgaag ttagccgagc acggtgcgca
    10501 tgtggctt
//
LOCUS       DATGFG010001111         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1215224, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001111 DATGFG010000000
VERSION     DATGFG010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215224"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VKQ52_21075"
     CDS             <1..972
                     /locus_tag="VKQ52_21075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLZ89765.1"
                     /translation="IRTQSPSTNSHLVMGPWYHGEWASNDGTHLGNVQWGANTSFWYQ
                     NTIEIPFFNYYLKGKGTEPKLPAATVFFSGANEWHQFDQWPPAASQEQSIYLQPDGSL
                     AWQAPAAKNSFSEYISDPAHPVPYTEDVHFSRTREYMTDDQRFASRRTDVLTFQTPVL
                     QEDLTLAGPVIADLKVSLSSTDADFVVKVIDVFPDDFSYTPVGPLPVRGHDNPPSYPM
                     GGYQMLVHGEIMRGRFRESFETPKPFTPGKVETVKFSIPDVAHTFRKGHRLMIQVQSS
                     WFPLVDRNPQKFVDIYTANDSDFQKETIRVFHDGDHSSRIILPVLKQ"
     gene            974..>2088
                     /locus_tag="VKQ52_21080"
     CDS             974..>2088
                     /locus_tag="VKQ52_21080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLZ89766.1"
                     /translation="MLRPYLFSPWLLLLLPLLSTCQDRRPSEPNYAKESYDKTEVSIP
                     MRDGKRLFTVIYSPKDHSHSWPFLMERTPYSAGPYGDTLYKGSLGPSGRLMRGGYIFV
                     YQDVRGRYLSEGDFQEMTPARDHVNRSKQTDESTDTWDTIEWLLKHVQNNNGRVGLWG
                     VSYPGFYASASLPDAHPAIKAVSPQAPVTDEFVGDDANHNGAFFLLDNFDFDNFFDIP
                     RPAPVKNYGRTLFRAEIRDAYQFFLNLGPLKNSNNAEYFNHQGKIWDEYLGNSTYDAY
                     WQARNIRTHLKNIRPAVLVVGGWFDAEDMFGSLRTYEAIEKQSFPNDNHLVMGPWTHG
                     AWGGSTWTKFGALDFQQNVNEYYHEIESEFFNHYLKDS"
BASE COUNT          474 a          649 c          540 g          425 t
ORIGIN      
        1 atcaggaccc agtctccgtc gaccaacagc catctcgtga tgggtccctg gtatcatggc
       61 gaatgggcat cgaatgacgg aacccatctc ggcaatgttc agtggggcgc caatacctcg
      121 ttctggtacc agaataccat cgagatccct ttctttaatt attacctgaa gggcaaaggc
      181 acggagccaa aactccccgc cgctaccgtc ttcttcagcg gcgcgaacga atggcaccag
      241 ttcgaccaat ggcccccggc cgcatcgcag gaacaatcca tctacctgca accggacggc
      301 agcctggcct ggcaggcgcc ggccgcgaag aacagcttta gcgaatacat cagcgatccg
      361 gcccatcccg taccctatac ggaggacgtc catttctccc gtacacggga atatatgacc
      421 gacgaccagc gcttcgcttc ccgccgcacc gatgtcctca ccttccagac gccggtatta
      481 caggaagacc tcacccttgc cggccccgtc atcgccgacc tgaaggtaag cctcagcagc
      541 accgatgcgg atttcgtcgt aaaggtgatc gatgtgttcc cggatgattt cagctatacg
      601 cccgtgggcc cgctgcccgt gcgcggtcat gacaacccgc cctcctaccc catgggcggc
      661 taccagatgc ttgtccatgg cgagatcatg cgcggccgct tccgggaaag ttttgaaacg
      721 ccaaaaccct ttactcccgg caaggtggag acagtgaagt tctccatccc cgatgtagcg
      781 catacgttcc ggaaggggca ccggctgatg atccaggtgc agagttcctg gttcccgctt
      841 gtcgaccgga atccgcaaaa atttgtggac atttacacag ccaacgacag cgatttccag
      901 aaggagacga tccgcgtatt ccatgacggc gatcattctt cgcggatcat cctgccggtg
      961 ctaaaacaat gatatgctca gaccttacct attctctccc tggctgctgc tcctgcttcc
     1021 cctgctgagc acctgccagg accgcaggcc ttcggagccc aactatgcaa aagaatctta
     1081 cgacaagacc gaggtctcta tccccatgcg cgacgggaag cggctcttca ccgtgatcta
     1141 ttcgccaaag gatcatagtc attcctggcc cttcctcatg gaaaggaccc cctattcggc
     1201 gggcccctat ggggatacgc tttacaaagg gtcgctgggc cccagcggca ggctgatgcg
     1261 cggcggctat atcttcgtct accaggatgt acggggccgc tatctcagtg aaggcgattt
     1321 ccaggagatg acgcccgcca gggatcatgt caaccgatcc aaacagacgg atgagagcac
     1381 cgacacctgg gatacgatcg agtggctgct aaagcatgtg caaaacaaca acggaagggt
     1441 cggcctctgg ggtgtctcct atcccggctt ctatgcctcc gcctcgttgc ccgatgctca
     1501 tccggccatc aaagcggtct ctccccaggc gcccgtcacg gatgaattcg tcggcgacga
     1561 cgccaatcac aacggcgcct tcttcctgct ggacaatttc gacttcgata atttcttcga
     1621 catcccgagg ccggcgcccg taaagaacta cggccggacc ctgttccgtg ccgagatccg
     1681 ggacgcctac caattctttc tcaacctggg gccgctaaag aacagcaaca atgcggaata
     1741 tttcaatcat caggggaaga tatgggatga gtatcttggt aactccacct acgatgctta
     1801 ctggcaggcg cgaaatatcc ggacgcattt gaagaacatc cggcccgccg ttcttgtggt
     1861 aggcggctgg ttcgacgcgg aggatatgtt tggctcgctg cgaacctacg aggcgataga
     1921 aaaacagtca ttccctaacg acaatcacct ggtgatgggg ccctggaccc atggggcctg
     1981 gggcggcagt acctggacaa aattcggcgc cctcgacttc caacaaaacg tcaacgaata
     2041 ctaccacgag atcgaaagcg aattcttcaa ccattacctg aaggattc
//
LOCUS       DATGFG010001112         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_67365, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001112 DATGFG010000000
VERSION     DATGFG010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67365"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VKQ52_21085"
     CDS             <1..272
                     /locus_tag="VKQ52_21085"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89767.1"
                     /translation="SAGLLFNVFSEYDPHNLLIRQAYNEVMDQQMEERRLRNMLERIS
                     RGRIVLRWPTKLTPFCFPLKVDSMREDLSSEKLEDRVRRMQAQLR"
     gene            complement(303..800)
                     /locus_tag="VKQ52_21090"
     CDS             complement(303..800)
                     /locus_tag="VKQ52_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89768.1"
                     /translation="MADFNRPVEPQSFLEEPRKIPEMLNVLTILTFIGSGLGVIGALW
                     GFVKAKSSYDTLAAMNLDQMPGFAKRMMGSDPLETARKAYENRLPILLLGLIGCALCI
                     YGAVQMRQLKKNGFTLYAVGEVLPIITSLIFLGSGATGVTYIIGYAIYVLFIVLYSTQ
                     MKYLR"
     gene            900..1688
                     /gene="pdeM"
                     /locus_tag="VKQ52_21095"
     CDS             900..1688
                     /gene="pdeM"
                     /locus_tag="VKQ52_21095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HLZ89769.1"
                     /translation="MQPAFPYRIRGQQLWLSADRCIFWEEEQSLIVSDLHFGKTGHFR
                     KAGIAVPQSVYREDLLRLLCQIQYFRPRQLLVVGDLFHSRENKELELFLKWREDFPHL
                     GIRLIEGNHDILHKDWYERAAIEVNPGVLTMGPFSFVHDIAEAAGGDTYYFSGHLHPG
                     IRITGMGKQHLQFPCFYFGAGYAVLPAFGRWTGTVSIDPAPEDHVFAILPPPGATPGG
                     GMPIGRRPMGGVRATRRDGGERNTVTTRPRSGGWIGPGTIFQIQ"
     gene            complement(1730..2482)
                     /locus_tag="VKQ52_21100"
     CDS             complement(1730..2482)
                     /locus_tag="VKQ52_21100"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HLZ89770.1"
                     /translation="MSIAGQNLKYLRKLRGWTQEEFAAKLGIKRSLLGAYEEERAEPR
                     LEVLETVSDMFKLTLDELLRKELGDSKGGSYLSKRRAQKLIAASTDIQLVPVKAAAGY
                     LAGYADPEFLDELNTFTLPMLAPGTYRAFEIVGDSMLPTPSGSVIVGEKVDDLEEIKS
                     NQTYVVVSRNEGIVYKRVMKNNKSKNKLTLISDNTAYQPYSVNSDDVIEVWKAQMIIS
                     KANTQQRWDVNQLANLVTNLQEQVTTLKKKMN"
BASE COUNT          595 a          671 c          842 g          691 t
ORIGIN      
        1 cttcggcggg gcttttgttc aacgtgttta gtgaatacga tccgcacaac ctgttgatcc
       61 gccaggccta caatgaggtg atggatcagc agatggagga gcggcgactg cggaatatgc
      121 tggagcgcat cagcaggggc cgtatcgtcc tgcgctggcc gacgaagctg acgcctttct
      181 gtttcccgtt gaaggtggac agtatgcggg aggacctgtc atccgagaag ctggaggaca
      241 gggttcgccg gatgcaggca cagttacgat aggggcccgt tggcgcttat gcgggccggt
      301 tgtcagcgca gatatttcat ctgggtggag tacagtacga taaagaggac gtagatggca
      361 taaccgatga tataggtgac cccggtggcg ccggatccca ggaagatcag gctcgttatg
      421 atgggcagca cctcgccgac ggcatagagg gtgaagccgt tcttcttcaa ctgccgcatc
      481 tgtacggcgc catagatgca aagggcgcag ccgatgagtc ccaggagcag gatcggtaag
      541 cggttctcgt aggctttgcg ggccgtttcg agcggatcac tgcccatcat ccttttggcg
      601 aagccgggca tctggtccag gttcattgcc gccagggtgt cataggagga ctttgccttg
      661 acaaagcccc agagggctcc gatgaccccg aggccgctgc cgataaaggt taggatggtc
      721 agtacgttga gcatctccgg tatcttgcgg ggctcttcga ggaaggattg gggctctacc
      781 gggcgattga agtcggccat gatggttaag tttagtctgt ctaagtaaat acaaatttca
      841 ggaatgacca agcaaatggg cagttttcag ttacatttgt aaacataggg gacagatcta
      901 tgcaacctgc atttccatac cgtatcaggg gacagcaatt atggctttcg gccgatcggt
      961 gtattttttg ggaggaggaa caatcactta tcgtttcaga cctgcatttt ggcaagaccg
     1021 ggcatttccg caaggcgggg atcgcggtgc cacagtcggt ataccgggag gatctgttgc
     1081 gtctgctttg ccagatccag tatttccggc cgcggcagtt gctggtggtg ggcgatctct
     1141 ttcacagccg ggaaaataag gagctggagt tgttcttgaa atggcgggag gatttcccgc
     1201 atttagggat ccggctgatc gagggtaatc atgatattct gcacaaggac tggtatgagc
     1261 gggccgcgat cgaggtgaat ccgggtgttt tgacgatggg gccgttctcc ttcgtgcatg
     1321 atatagcgga ggccgccggt ggcgacacct attatttttc gggccatctt catcccggca
     1381 tccgcatcac ggggatgggt aagcagcatc ttcagtttcc ctgtttctat ttcggggcgg
     1441 ggtatgcggt gttgccggcc tttgggcgat ggaccggtac ggtgtcgatc gatccggcgc
     1501 cggaagacca tgtttttgcg atcctgccgc ctcccggtgc gacacccgga ggcgggatgc
     1561 ccatcggcag gagacccatg ggcggggtac gtgctacgag gagggatggg ggtgagagga
     1621 atacggtgac gacgaggccc cgtagcggcg gctggatagg gccgggcacg atattccaga
     1681 tacaatagat gccagaatac ccctggttca ttcagacgcc agaataccct tagttcattt
     1741 tctttttcag ggtagtgacc tgttcctgca ggttagtgac caggttggcc aactgattga
     1801 catcccaacg ttgctgggtg ttggccttgc tgatgatcat ctgggccttc cacacttcaa
     1861 tgacatcatc ggagttgaca ctatagggct gatacgccgt attgtcacta ataagcgtga
     1921 gcttgttctt ggatttgttg tttttcatca ccctcttata gacgatccct tcgttgcggg
     1981 atacgacgac ataggtctgg ttgcttttga tctcttccag atcgtccact ttttcgccga
     2041 cgatgaccga accgcttggg gtcgggagca tggaatcccc gacgatctcg aaggcgcgat
     2101 acgtgcccgg agcaagcatg gggagggtga aggtgttgag ttcatcgagg aattcaggat
     2161 cggcataccc ggcgagataa ccggccgcgg cctttacggg aaccaactga atgtcggtgc
     2221 tggcggcgat gagcttctgg gccctgcgct tggagagata actgccgccc ttgctgtccc
     2281 ccaattcctt tcgtaagagt tcatcgaggg taagcttgaa catatccgac accgtctcga
     2341 gcacttccag acgtggttct gccctttctt cctcgtaagc gcccagcaaa gagcgcttga
     2401 tcccgagttt ggcggcaaat tcttcctgtg tccagccccg cagcttgcgg agatatttga
     2461 gattttgtcc tgcgattgac atgggctaat atatttagca ctaagatact aatatattta
     2521 acaatgcaaa aaaaatattt tttggtccgg ggacccgcag tggggatgcg gggggaggaa
     2581 atgccccttc aaaggggggt ggatagctgc ttttttgtac cttcgccttc cctgtcaatg
     2641 taaatcctgc ttccctgtca atttgacctt ccggcaagga tagcatcaaa tttgaccttg
     2701 aaccggggct cccgggcctg ccggaaaggg cagatggggg taagaaaact ataaaatcat
     2761 taatacttaa ggaattatgt taggtattct atccaaaat
//
LOCUS       DATGFG010001113         4079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1036667, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001113 DATGFG010000000
VERSION     DATGFG010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036667"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2110)
                     /locus_tag="VKQ52_21105"
     CDS             complement(<1..2110)
                     /locus_tag="VKQ52_21105"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89771.1"
                     /translation="MPSKMACFVGRKGMIQNYFKVAFRTLFRNKGYTLLNGAGLSVGI
                     GACLMIFLLVRFELSYDNFHPDRDRIYRLVSAPYRPGSPLGMGAGAPLPAAQALRLEY
                     PQLEKVAAIFGRDGQVTVLDDKKEAGKKFNEEGDVYFAESAFFDIFRFHWLAGDPRTA
                     LTAPNTVALSRETAVKYFGDWRTGVGRVIKFNNKEIYQVTGILQDVPANTDFPLKVVF
                     SYSSLTNVDLTDWQGIYGRGYTFIRLPRGLAASRMNAELRDLVNRHTAAGRERRGIEL
                     QPLADMHFDGRFGNYSGRTFSRELIASLCLMAAFLLIIACVNFVNLATAQAVRRSQEV
                     GIRKVLGSTRGRLIRQFMGEAGLITLLAVAAALCLTATALPLLNDLLKTDITLQLSDP
                     PLLGFLSLVTMAATLMAGLYPALFLSRCSPVTSLKNKLTTNTPGGISLRRGLVVLQFC
                     IAQVMIICVLVVSGQTAYFRHASLGFDKASIVNVPIPNDSLSQLKMNTVRQELLRIPG
                     VQSVSFSTFSPLDNDIWSNQFKFDHSKTKTDFQAYFKWADADFFQTYQPVLVAGRLYG
                     PSDTLREFVVNETLVKKLGLRQPKDILGKEINFWDERRAPVVGVVRDFHTNSMQDPIR
                     PVVMGVWKETYGMAGIKLRPANVGQSLAAIEKVWNAAYPDAVYSYQFLDEKIDNYYKE
                     EGKLSVLYRVFAGIAILISCL"
     gene            complement(2190..2582)
                     /locus_tag="VKQ52_21110"
     CDS             complement(2190..2582)
                     /locus_tag="VKQ52_21110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLZ89772.1"
                     /translation="MKNAISWFELPATDLDRAQKFYETILDIRMIPLESPHIRMRMFP
                     IEDPMTGIGGALALAPDIYKPSATDGPIVYLNANPDVQRVLDKVEPAGGKIIVPKTEI
                     SPEYGHMALFIDTEGNRVALHSIPPPRG"
     gene            2722..>4079
                     /locus_tag="VKQ52_21115"
     CDS             2722..>4079
                     /locus_tag="VKQ52_21115"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HLZ89773.1"
                     /translation="MIATFTRILTVLTVLLLSLQGKTLATTFYVNSSNPTPGSGLTWA
                     TAFNDLNAALAAAESSAAFTNTIWVAKGTYKPTTTTDRTATFLLKGPLWVYGGFIGTE
                     TALGQQDPVANPTILSGDIGVPGDPSDNSYHVVTFENYNSGTWMNGFTIEYGNANAGY
                     PASTTPQADNTGGGVLLLTNPNDNNVGWMQYCTITNNFAVYGGGYGAYGDGTGTTGGN
                     TFSYYKVNNCTFTNNSAWMGGAFGVVAANQVWFSGTIQNCIFTNNSSTGGQGSVVASV
                     ANNAYSNVTNNFNNSVLYNNPQPIAYNQSTSSATPAGKSYIWITNDIVWQSGAPYAGP
                     LNTGPNSTDIDFSNCDLDLVTPLGSNLDADPQFINAAGGDFHVAHCTSPVIDQGWPVG
                     NMVNYDMDGNPRVQGTAIDIGAYEQSHPLAPTVPNPTVSYCPNATAAPLTATAAAGNT
                     LL"
BASE COUNT          940 a         1152 c         1073 g          914 t
ORIGIN      
        1 cgagacagga gatgaggatg gcaatgcccg caaataccct gtacagtacc gacaattttc
       61 cttcttcttt gtagtaatta tcgatcttct catcgaggaa ttgatatgaa tacaccgcat
      121 caggataggc cgcattccag accttttcaa ttgccgccag ggactgccca acattcgccg
      181 gccttagctt gatgccggcc atgccatagg tttctttcca tacgcccatg accaccggcc
      241 tgatggggtc ctgcatggag ttggtatgaa aatccctgac aacgcccacc acgggcgccc
      301 gccgttcgtc ccagaaattg atctctttcc ccaggatatc cttcggctgg cggagcccca
      361 gttttttgac cagggtctcg ttgacgacga attcccgcag cgtgtcactc ggcccataaa
      421 gcctgccggc aaccagcaca ggctggtagg tttggaagaa atcggcgtcg gcccatttga
      481 aataagcctg gaagtctgtt ttggttttgg agtggtcgaa tttgaattgg ttgctccata
      541 tatcattatc caaagggctg aaggtgctga aactcacgga ctggacaccc ggaatgcgca
      601 ggagttcctg acggacggtg ttcatcttca actggctcag gctgtcattg gggatgggta
      661 cattcacgat ggatgctttg tcaaaaccca gggaggcatg cctgaaatac gctgtttgac
      721 ccgatacgac cagcacacag atgatcatca cctgggcgat gcagaactgc aacaccacca
      781 gcccccgccg cagggagatg ccgcccgggg tattcgtcgt gagcttgttc ttcagcgatg
      841 tgaccgggct gcatcgggaa aggaataggg cagggtagag cccggccatc aacgttgccg
      901 ccatcgtgac cagcgagagg aatcccagta agggtggatc gcttagttgc agggtgatat
      961 cagtcttcag caggtcgttc aacaacggca gcgccgtcgc cgtcagacaa agggccgccg
     1021 ccaccgctaa aagcgtgatc aggccggcct cgcccatgaa ttgccggatc aaccggccgc
     1081 gggtactgcc gagtaccttc cggataccta cctcctgcga acgcctgacg gcctgggccg
     1141 tggccaggtt gacaaaattc acacaggcga tgatcaaaag gaaagccgcc atcaggcata
     1201 aagaagcgat cagttccctg ctgaaagtcc ggccgctgta attgccgaat ctgccgtcga
     1261 aatgcatatc tgccaacggc tgcaactcga taccccgccg ctcgcgcccc gccgccgtat
     1321 gccggttgac gagatcccgc agctccgcat tcatgcgtga agcggcaaga ccccgcggaa
     1381 gccggataaa tgtgtatccc ctgccataaa tcccctgcca atcggtgagg tccacattgg
     1441 tcagcgagga ataggagaac acaaccttta gcgggaaatc ggtattggcc gggacatcct
     1501 gcaggatgcc cgtcacctgg tagatctcct tgttgttgaa tttgatcacc ctcccgacac
     1561 ccgtcctcca gtccccgaag tatttgacgg ctgtctcccg gctaagggca accgtatttg
     1621 gtgcggtcag ggccgtacga gggtctccgg cgagccagtg gaagcggaag atgtcgaaga
     1681 aggcggattc ggcgaaataa acatcccctt cttcattgaa ttttttgccg gcctccttct
     1741 tatcatccaa tacagtcacc tgcccatccc tgccgaatat ggccgccact ttttccaact
     1801 gcggatattc caatcgaagg gcttgcgctg ccggcagcgg ggcgccggct cccatgccta
     1861 acggcgatcc cggtcgatag ggggcactca ccagccggta gatacgatcc cggtcaggat
     1921 ggaaattatc atagctaagc tcgaatcgta cgagaaggaa gatcataaga cacgctccaa
     1981 tgccgacaga cagaccggcc ccgttcagga gggtgtaacc cttattccgg aaaagcgtcc
     2041 ggaacgctac cttgaaatag ttttggatca ttcccttacg gcccacaaaa catgccattt
     2101 tggaaggcat tgtggcttaa atatttgtga ctgtctttcc cccggtctgt ccgcttctga
     2161 tacaatcgat gcccgcttgc gccgggcctt tacccgcggg gtggtgggat actgtgcagc
     2221 gctaccctgt taccctcggt atcgatgaat agggccatat gcccgtattc cggcgagatc
     2281 tccgttttgg ggacgattat ctttcctccg gccggttcaa ccttatcaag cacccgctgc
     2341 acatcgggat tggcgttcag ataaacgatg ggcccgtccg tcgcggaagg tttatagata
     2401 tccggagcca gggccagggc gccgccgatg ccggtcatgg gatcctcgat ggggaacatc
     2461 cgcatccgga tatgtgggct ctccagcgga atcatccgga tatccaggat agtttcgtag
     2521 aatttctgtg cacggtcgag atcggtggcc ggcaactcga accaactgat ggcattcttc
     2581 atagtcattt gcaaacgttt taaccgaaga tagatcaaac ttccgaaatt gagccattgt
     2641 actacacaat gcaataattc acttaggggt ggcgtttgca tcgttcgaag ctaacgctga
     2701 cacctaattg caaccgctac tatgatcgcc acctttaccc gaattctcac tgttcttact
     2761 gtcctcctct tatccttgca aggcaaaact ttagcaacca ccttctacgt caatagtagc
     2821 aaccccacgc ccgggtcagg gcttacctgg gccacggcct ttaacgatct gaatgccgca
     2881 ctggctgccg ctgaatcttc ggcggccttt accaatacca tctgggtagc taagggaaca
     2941 tataaaccca ccacgactac cgatcggacg gccaccttcc tgctaaaagg cccgctttgg
     3001 gtctatgggg gcttcatcgg tacggaaacg gccctgggcc agcaggatcc ggtggccaac
     3061 cctaccattc taagcggaga tatcggcgta ccgggagatc cctcggacaa ctcctatcat
     3121 gtagtcacct ttgaaaacta taatagcggc acatggatga atgggttcac gatcgagtat
     3181 ggaaatgcca atgccggtta ccccgccagt acgacccccc aggcagataa caccggaggt
     3241 ggagtactgc tgctgacaaa tccgaatgat aacaatgtcg gatggatgca gtattgcacc
     3301 atcaccaaca attttgccgt ttatgggggc gggtatggcg cctatgggga tgggacgggt
     3361 accaccggcg gcaatacatt tagctactat aaggtaaata actgcacgtt caccaataac
     3421 agcgcgtgga tgggcggcgc ctttggggtg gtggccgcga accaggtctg gttttcgggg
     3481 acgatccaaa actgtatttt caccaataac agcagcactg gcggacaagg ttcggttgtc
     3541 gcctcggtgg cgaataatgc ctattccaat gtgaccaata attttaataa tagtgtcctc
     3601 tataataatc ctcagccgat tgcttataat cagagcacca gttccgctac tccggccggt
     3661 aaaagttata tctggatcac caatgatatt gtctggcaat ccggcgcccc ttatgcaggg
     3721 ccattaaaca cgggacccaa tagtaccgat atcgatttct ccaactgcga cctcgatctg
     3781 gtgacgccat tgggcagcaa cctggatgcg gatccccagt tcatcaatgc ggcgggcggc
     3841 gattttcatg tggcgcattg taccagcccg gtgatcgatc agggctggcc ggtaggaaac
     3901 atggtaaatt acgatatgga tggaaatccg cgcgtccagg gtactgccat cgatatcgga
     3961 gcctatgagc aatcccatcc cctggcgccc acagtaccta atcccactgt ctcctattgt
     4021 ccgaacgcca cggcggcccc cctgacggct acggcggctg ccggtaatac gttgttgtg
//
LOCUS       DATGFG010001114         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1326291, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001114 DATGFG010000000
VERSION     DATGFG010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326291"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VKQ52_21120"
     CDS             complement(<1..734)
                     /locus_tag="VKQ52_21120"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLZ89774.1"
                     /translation="MKEEVVLQVAAKTSVGRIRELNEDNFIVTNNISGADWFLPQDAY
                     ANGVMGTVMVVADGMGGTNAGEVASKIAVDSVQKYFNALSSSPLKDGRAGAIVRDSIL
                     YAHRAIVNHAMTHRETEGMGTTLVLCWILGGRAYVGWSGDSRCYVWHPVRGLRQLSKD
                     HSYVQSLVDEGKITNEEAFIHPQNNIVLQSLGDGTIEPEPDVTGVPIGKDDLLLLCSD
                     GLSGMLTDAELEEILRKTQGDVGLAV"
     gene            complement(786..>2097)
                     /locus_tag="VKQ52_21125"
     CDS             complement(786..>2097)
                     /locus_tag="VKQ52_21125"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLZ89775.1"
                     /translation="YIELGRGSGFAVSYGEDCRTVSRPHAAIVRRGDIWVLKPLSKNN
                     PTLLNKSPVRDEAPLRNGDEIQLSYEGPKLLFLLPPNNLVGTLGMTARMRAVMNEGVR
                     PYRKAITLIILLFLVVTGGLGWYIYHQGEAYKVDIARVREEARHNLDAIRKESADTIA
                     AMKKALLSATQLRTDRTTPNHHSPSADEGAAPSALKDLYPGVYYLRTAKVVMDVDGKQ
                     MEAKYAISGTGFLLNDGRFVTARHVVQPWFFLESGASDLEKALNIVASNGGKVTHYFT
                     AYSTDGQQIELNGDEFTVNNEEDETEKVTLESGEELNSRVAHPSDKDWAVARLSSSMH
                     TGLVCDREMSRNLHASSQVFALGFPFGIGANGQGDIHPSYGPCTVSMDGLNKGVIMVS
                     GRSFDHGDSGGPVFAFADNKYYVVGIVSAGAGAQGIIVPVSALP"
BASE COUNT          466 a          757 c          445 g          429 t
ORIGIN      
        1 ctcaccgcaa gacccacatc cccctgcgtc tttcgcagga tctcctccaa ctccgcatcc
       61 gtgagcatcc cgctaagccc gtcactgcaa agcaacagca ggtcatcctt cccgatcggt
      121 accccggtaa catcgggctc cggctcgata gtcccatccc caagactctg caggacaata
      181 ttgttctgtg gatgaataaa agcctcctcg ttcgtgatct taccctcatc gacaagcgac
      241 tgcacatagg aatgatcctt gctcagctgc cgcaacccac gcaccggatg ccagacataa
      301 cagcggctgt cgccactcca ccccacatag gccctcccgc ccaggatcca gcaaagcacc
      361 agcgtcgtac ccataccctc cgtctcccgg tgcgtcatcg catgattcac gatcgcccgg
      421 tgcgcataca ggatcgagtc ccttacgatc gcacccgccc gcccatcctt caaaggcgaa
      481 gaactcaacg cattgaaata tttctggaca ctgtctaccg cgatcttcga agccacctcc
      541 cccgcattcg tcccccccat cccatcggca acaaccatca ccgtacccat caccccgttc
      601 gcatacgcgt cctgcggcag aaaccagtcc gccccgctga tattattggt gacaataaaa
      661 ttatcctcgt tcaattccct tatccgcccc acgctggtct tcgcagccac ctgtaacacg
      721 acttcttcct tcatatacgc atggttttat ttgttgttct ataaggctaa ccctccggat
      781 caaactcagg gaagggcgga caccgggacg atgatcccct gcgcacccgc accggccgac
      841 acgataccga ccacataata tttgttatct gcaaacgcaa acaccggccc gcccgaatcc
      901 ccatggtcaa aacttctgcc cgataccatg atcactccct tgttaaggcc atccatggag
      961 accgtacagg gaccatacga aggatggata tccccctgcc cgttcgcgcc gatcccaaaa
     1021 ggaaaaccca gcgcaaacac ctgcgagctc gcatgcaggt tccgtgacat ctcccggtca
     1081 cacaccagcc ccgtatgcat cgacgagctc aagcgcgcca ccgcccagtc cttatcactg
     1141 ggatgcgcca cccggctgtt cagctcctcc ccgctctcca gcgtcacctt ctccgtctca
     1201 tcctcctcgt tattgaccgt aaactcatcc ccattcagtt ctatctgctg cccgtctgtg
     1261 gaataggccg taaaataatg cgtcaccttc ccgccattgc tcgcaacgat attcaacgcc
     1321 ttctccaggt cgctggcgcc cgactcgaga aagaaccagg gctgcaccac atgccgcgcc
     1381 gtgacaaacc gcccgtcgtt aagaagaaaa cccgtacccg aaatggcata cttggcctcc
     1441 atctgcttgc catccacatc catgaccacc ttggccgtcc gcaggtaata taccccggga
     1501 tacaggtcct tcagcgccga aggcgccgca ccctcatccg ccgaaggcga atgatgattg
     1561 ggcgtcgtcc ggtccgttct cagctgcgta gccgacaaaa gcgccttctt catcgccgcg
     1621 atcgtatccg cactctcctt ccggatggca tcgagattat gcctggcctc ctcgcggacc
     1681 ctggcaatat ccaccttata ggcctccccc tggtgataga tataccagcc caggccgccg
     1741 gtcacgacaa gaaaaagcag gatgataagg gtgatggcct tcctgtacgg cctcacaccc
     1801 tcgttcatca cagcccgcat ccgggccgtc atccccagcg taccgacaag attattgggt
     1861 ggtaataaaa aaagcagctt cggcccctca tacgacaatt gtatctcatc gccattccgc
     1921 agcggcgcct cgtcccgtac cgggctcttg ttaaggaggg tgggattatt cttcgacaag
     1981 ggctttaata cccagatatc tccccgccgc acgatcgccg catgcggccg gctcaccgtc
     2041 cggcagtctt ccccataact tacggcaaaa ccactgcccc tccccagctc gatatag
//
LOCUS       DATGFG010001115         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_297162, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001115 DATGFG010000000
VERSION     DATGFG010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297162"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VKQ52_21130"
     CDS             complement(<1..984)
                     /locus_tag="VKQ52_21130"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HLZ89776.1"
                     /translation="MRKLYLEFIRGAASVCVLLYHLPETWFAGGGYHRFSLAAVGSDA
                     VAIFFILSGCVINISYTRKRVSKKEFISSRLLRIYPQFFLGVLFALIVTVSFRRPWPP
                     PGQLLGNILMLSTLQGFIVQSLRGNSVVWSLTFEMFFYLVFMLSIGRFRKEILRGWFV
                     LALLMIPCCYFLPGIGLIDHFIAMFAFSAIWLVGYYVYEHRDLFYVDGYTAALSLGTL
                     PIVSRLQFAFQWPGVDALKHFLFALIAIPFFRYCLQTGPSGKKLPRLVPLLVYLGMSI
                     LLFRETSLSTATRAACAVAPLAMMSLYWLIHRAGFREKAVSVVNRVGSILGK"
     gene            complement(1097..2371)
                     /locus_tag="VKQ52_21135"
     CDS             complement(1097..2371)
                     /locus_tag="VKQ52_21135"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HLZ89777.1"
                     /translation="MSLKRREFLRLSAGKATLGSALLYGLPAWMKKTDNPLDTLTPMT
                     GDVVPISVAERQARIQKAQRLMTENRIQAIVLDSGTSMVYFTGMSWGGSERPMVAIIP
                     AQGDVKYVCPAFEAERLRELITIGTEVRVWEEHENPYKVIAGILRDYGIRTGNVGMEE
                     RLRFFIFDGVRKEASQLNYVSADPVTATCRMIKSPAEIALMQKANDITVSAYKASIAL
                     LHEGMTPADFSSIAEDAFSRLGVRGGLSANFGQASAFPHGSIQTQHLKKGDVVLMDDG
                     CKVQGYCSDISRAIVFGAEPTKRQREIWELEKKAQAAGFAAAKVGAPCENVDAAARKV
                     ITDAGFGPGYKIPGLPHRTGHGIGMDIHEWGNMVMGNKTPLQPGMCFSVEPMVAIYGE
                     FGIRLEDCAYMTEDGPRWFSEPSPSIDKPFAS"
     gene            2432..>3040
                     /locus_tag="VKQ52_21140"
     CDS             2432..>3040
                     /locus_tag="VKQ52_21140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89778.1"
                     /translation="MIKSLLTVALRNFKRDKWYSLLNILGLMIGITFSLFLIFYIKDE
                     LSFDRYNDKADRIYRISSNIKEPEKDTMRWVSTQFPLGPTLKKDYPEVEEAVRFIAED
                     KKMYRKGELRIYEDKIFYADSNVFRVFSYPFLQGDPNTALVEPNSLVLTQSVAEKFFG
                     RSAGVVGQTLQDAKGDIFKVTGVIKDIPYNSHLRFNLLISRTT"
BASE COUNT          732 a          904 c          786 g          618 t
ORIGIN      
        1 cttcccgagg atgcttccta cccggttgac aacgctgacg gccttttccc tgaaaccggc
       61 acggtggatc agccaataca gtgacatcat cgcgagggga gcaactgcgc aggcagccct
      121 ggtcgccgtc gaaagactgg tctcgcgaaa caacagtatg ctcatgccca ggtagaccag
      181 gagaggaaca agccgcggca atttttttcc cgatggcccg gtttgcaggc aataccggaa
      241 aaaagggatc gcaataaggg caaacaaaaa atgcttcaat gcatccaccc cgggccattg
      301 aaaggcaaac tgtagccgcg agacgatggg caatgtgccg agactcaacg cggccgtgta
      361 cccgtcgaca tagaacagat cccggtgttc atagacataa tatcccacca gccagatggc
      421 ggagaaagcg aacatggcga taaaatggtc gatcaatccg atgcccggga gaaaataaca
      481 acaagggatc atcaataagg cgagcacgaa ccagccccgc aggatctctt tacggaaacg
      541 gccgatggag agcatgaata ccaggtagaa aaacatttcg aaggtgaggc tccataccac
      601 ggagttgccg cgcagcgatt gaacgataaa tccctgcagg gtgctcagca tgaggatatt
      661 gcccaacaac tgtcccggag gaggccaggg ccgcctgaag gacacggtca cgatcagggc
      721 gaacaggacc ccaagaaaaa attgcggata tatccgcaaa agccggctgg atataaactc
      781 cttttttgac acccgcttcc gggtatagga gatattgatg acgcaacccg aaagaataaa
      841 gaatatggcc accgcatccg aaccgacggc ggccagggaa aaacgatgat atcctccccc
      901 cgcgaaccag gtctcgggca agtgatagag caaaacgcaa acggatgccg caccccttat
      961 aaattcaaga tagagctttc gcatttagtt tcctctgaaa ctaaagtaga acactgcagt
     1021 catactgcac ctgatcttcc tcaccctctc tgatgaacct ggtcatgcgg ctccggtccg
     1081 cggtctttcc agatcactat gacgcgaacg gcttgtcgat ggacgggctg ggctccgaga
     1141 accatcgcgg cccatcctcc gtcatatagg cacagtcctc gagacggatg ccgaattctc
     1201 cgtagatcgc gaccatcggc tctacgctaa aacacatccc cggctgaagc ggcgtcttgt
     1261 tgcccatgac catatttccc cattcatgga tatccatccc gatgccatgt cccgtccggt
     1321 gcggcagacc cgggatcttg tagccggggc caaaaccggc atcggtgatc accttccgcg
     1381 ccgcggcatc gacattctca cagggggcgc cgacctttgc cgccgcaaac cccgcggcct
     1441 gggccttttt ctcgagttcc cagatctcgc gctggcgttt ggtcggttcg gcgccaaaaa
     1501 cgatggcccg gctgatatcg gagcaatagc cctgtacctt gcacccatcg tccatcagga
     1561 cgacatcccc ctttttcagg tgctgggtct ggatgctgcc atgggggaaa gccgaagcct
     1621 ggccaaagtt ggcgctcaac ccaccccgga ccccaagacg ggagaaggca tcttcggcga
     1681 tcgatgagaa atccgcgggt gtcatgcctt catgcaggag ggcgatcgac gccttatacg
     1741 cggaaacagt gatgtcattg gctttttgca tcagggcgat ctcggccggc gacttgatca
     1801 tccggcaggt agccgtcaca gggtcggcgc tcacataatt gagctgcgat gcctcctttc
     1861 ttaccccgtc gaaaatgaag aaacgcaatc tttcctccat gccgacattc ccggtacgga
     1921 tgccatagtc cctgagtatc ccggcgatca ccttgtacgg gttctcatgc tcctcccaca
     1981 cccttacctc cgtgccgatg gtgatcagtt cccgcaaccg ctcggcttcg aaggccgggc
     2041 atacatattt aacatccccc tgcgccggga taatggctac catcggtcgc tcactccctc
     2101 cccagctcat tcccgtgaaa tagaccatgg acgtccccga gtccagcact atcgcctgga
     2161 tcctgttctc cgtcatcagc cgctgggcct tctggatccg ggcctgccgc tcggcaacgg
     2221 aaatggggac cacgtcccct gtcatcgggg tcagcgtatc caggggatta tctgtttttt
     2281 tcatccaggc gggcagccca taaagaaggg cgctgcccag cgtagccttc ccggcggaca
     2341 gccggaggaa ttcgcggcgt ttcagcgaca taaattcaag gtttgggacg gaaggtaggg
     2401 atttgttccc ttttttttgc atatttaaac catgataaag agcctcctca ccgtcgcact
     2461 gcgcaacttc aaacgggata aatggtatag cctgctcaat atcctgggcc tgatgatcgg
     2521 catcaccttt agcctgttcc tcatcttcta tatcaaggac gaactcagct tcgaccggta
     2581 caacgacaaa gccgaccgga tctatcgtat cagctcgaat atcaaagagc cggaaaagga
     2641 cacgatgcgc tgggtctcca cccaattccc gctggggccg acgcttaaaa aggactaccc
     2701 cgaagtggag gaggccgtgc ggttcatagc cgaagacaag aagatgtacc ggaaaggaga
     2761 gcttcgcatc tacgaggaca agatcttcta cgccgacagc aatgtcttcc gggtcttcag
     2821 ctaccccttt ctccagggtg accccaacac cgcgcttgta gagcccaatt cccttgtcct
     2881 gacccagtcc gttgccgaaa agttctttgg caggagcgcc ggtgttgtcg gccagacgct
     2941 gcaggatgcc aagggcgata tcttcaaagt taccggcgtc ataaaggata ttccctacaa
     3001 ttcccatctt cggttcaacc tcctcatttc gagaaccacg
//
LOCUS       DATGFG010001116        10325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1151229, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001116 DATGFG010000000
VERSION     DATGFG010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151229"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..414
                     /locus_tag="VKQ52_21145"
     CDS             46..414
                     /locus_tag="VKQ52_21145"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HLZ89779.1"
                     /translation="MQHPTIDIKRVYAAREKADGIRILVDRLWPRGIKKEEAHIEEWA
                     KDIAPSAELREWYGHDPDRWPEFQKKYKAELKHNESLDAFIEKYQHEKHLTLIYATKD
                     EQHTHALVLKDYLDHEFSHR"
     gene            complement(450..3881)
                     /gene="treS"
                     /locus_tag="VKQ52_21150"
     CDS             complement(450..3881)
                     /gene="treS"
                     /locus_tag="VKQ52_21150"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HLZ89780.1"
                     /translation="MAGSNVLDDALHWYKDAIIYELHIKAFCDGDADGVGDFQGLLQK
                     LDYLQDLGVTAIWLLPFYPSPLRDDGYDIADYYTINPSYGDVSRFRHFLREAHRRNLK
                     VITELVINHTSDAHVWFQRARQAPKGSKERSFYVWTDDPDQYKDVRIIFQDFENSNWT
                     WDPVAEQYYWHRFFHHQPDLNYDNPAVQEEVFRILDHWCRMGVDGFRLDAVPYLFERE
                     GTNGENLPETHVFLKRLRRHLDEHFPGTVLLAEANMWPEDSASYFGNGDECQMNYHFP
                     LMPRMFMALQMEDHYPITDIFDQTPEIPLTCQWAIFLRNHDELTLEMVTDEERDYMYK
                     VYARDPKARINLGIRHRLAPLLDNNRRKIELLNSLLFSLPGTPVIYYGDEIGMGDNFY
                     LGDRDGVRTPMQWSPDRNAGFSAANPQKLYLPLILDPEYHYESVNVETQRANTSSLFW
                     YMKRLIAVRKKFKTFGRGNLKFVNAENPKVLAFTRTYEEETLLIVVNLSKFSQAAEVD
                     LVPYKGFVPVDVFSRNRFPVIREDGQYFFTLSPYSFQWFVLEKAHPEMDEEKPLPLLL
                     LRQWAGLMEGTARRQLENTILPRYLVRAKWYIPRERNTYQVTVADYTVVEAGGRQVFW
                     LLLEIMGDDGVPGMYQLPVWVVDVEAGRRLGEVYPEALIATVRSEEGEGMLVDALFSS
                     EWQMALLQRLAAGHKGHSAGPVVFEGSVRVKEYLKGVSGAAPKIHTSDLYNTSIAYDG
                     VFFLKFYRKVDRVVNSDQELTRFLSKEAHFPYLPAFIGSIEWKDKRGCIVLGLLQEMI
                     ENHGDGYGFFLERVNNYIERIMAAEKSVPLPQERLGTLARPVAFEELPVELQALLGTH
                     AAEEVRLIGMRTAEMHLALASGTGKDFKPEPFSLHYQRSLFSYMQSLVRETYQNLGRN
                     VKSLPKDMRERLDRIVAYRPELLATLKKIYAKKLDTQKIRIHGNYHLGQILLTGKDIA
                     ISDYSGDPSLSYSERRLRRSPAVDLASMILSIHEVAFEGVFQGRQLHEGDVERMLPMA
                     GLWAHYVSGWFVRAYKERAGTLLPAREVDFETLLQYFLVQKAIGVFNGYLKRDPKRLV
                     IPQTILRSVLRPQPEAVTVVPQAEVVAPKAAELGPKPVGDAGEQVAAVTAGRGKG"
     gene            complement(3945..4472)
                     /locus_tag="VKQ52_21155"
     CDS             complement(3945..4472)
                     /locus_tag="VKQ52_21155"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HLZ89781.1"
                     /translation="MNLSIPVNRRRVPTALLIYSGITVAWVLLMVTQDFGPQYGFLFG
                     ISLVMLLVRVALLLVDYIKTLFDGRARLVISAEGIDDRRSIFSCGMIPWSDITAIELY
                     RRRKMKVLVVRLINAEAVLAAQPFWKRVFQREMSKRFGSPVLVSQSSVHYDLLLLHDE
                     LRTFVTYSPDHLLQK"
     gene            4822..6780
                     /locus_tag="VKQ52_21160"
     CDS             4822..6780
                     /locus_tag="VKQ52_21160"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HLZ89782.1"
                     /translation="MANGISFLKPSGLLLLAAFFVVLVPANGSPLTTPILAGQRLSLQ
                     PWLTVLEDSAGSATAGDLLRRPNAFVPLPSLQKGDPTSYYWLRADIRTNDLYDPGRIL
                     SFSHLTFVDVYLYDDTGLIVHKVAGAFRPRSNLADGDGRQYVMLPLEKNKTYTLLIRV
                     HHTKHYQPVFDFEMMPGRQYFNTLGTKEAFDAALMGAVTLFFVYTLLSWIVSRFRPYL
                     WLLLSIGGIGLYVIGMNGYWIDWFSPEHPAAGWLLNVHFLHLGMLGLYLLVTDFWHLK
                     QDFPGLYAWGRWIPLLLGVSSLACFGIDYFTGNYSLASNLTCAEHPLILAFILASLFI
                     CGPRLSAAQRYLAYGIALCSIAGVFVTLNAVINHERSLPSVSLVGDCIILTVFLLFST
                     GLKEEMRHHELAKQVALEELNRLQQHQNTILEKKVEERTDALRISNKRLLNQKHLLAE
                     RNTKIELLINELNHRVKNNLQLLYSLLSLQLPLVRDGVSRDILQGNIGKIRAMMLVNQ
                     KLFNFEKGRGIGLCEFITELAVHLQKIYDTKEKTRIVQNIPAGLRLSDKHTLSFGLIL
                     SELFTNTFKHAFRDQPDPCICVEAVAVTDQLLEFHYSDNGVGIPAGEQPEKFTMGIPL
                     IKDLTRQMNGQMTVRGDKGLSYSFTIPV"
     gene            6787..7392
                     /locus_tag="VKQ52_21165"
     CDS             6787..7392
                     /locus_tag="VKQ52_21165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HLZ89783.1"
                     /translation="MVRILIIEDEIIIARFIEHQLRANFACETRIAVSGKEARTAMTE
                     MLPHLLLCDIQLEDELSGIDLVTTLRRGYAFEVIYITSYQTHSIIEKAAATHPANYII
                     KPIDETQLFAGVQLVITRLDTGNQTGKPLLQADSLFTETELRILRLIREKKTTLEIAD
                     ALHLSRYTIKNHRHNICRKLELADENNALLKWVVQNGHLLK"
     gene            complement(7397..8248)
                     /locus_tag="VKQ52_21170"
     CDS             complement(7397..8248)
                     /locus_tag="VKQ52_21170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLZ89784.1"
                     /translation="MRNFFSFPQWLLGGFLLFSGACTNEPRQTGTTAPATAADSSGIR
                     EEHISFRADSSNLHAFIVYNDSVKGKRPAVIVIPEWWGLNDYPKMRARMLAGLGYVAM
                     ALDIYGDGKVADNPDSAGAYSRPFYMNPMKAKIRIDSAIAQLRRYDNVDPANIGAIGY
                     CFGGGVLLNSVRLGDDLKGAVSFHGSLIGTPARKDLLRTKILVCHGNDDKFVSPADVA
                     KFRKQMDSIGADYHFIGYDSATHAFTNPASTEAGKKFHLPIAYNAKADTASWAAMKQF
                     FAGVFGR"
     gene            complement(8290..8631)
                     /locus_tag="VKQ52_21175"
     CDS             complement(8290..8631)
                     /locus_tag="VKQ52_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89785.1"
                     /translation="MRKVYIPLVVLLLSIPACTTTLPTASLEDSYLMHVECRNDVGIR
                     QSVLSLLRQARARDIKEDNSTGILVIEAAYYRSETPVDLLERIGDDLRRMGMVTDVRL
                     EENHSVIRQAP"
     gene            complement(8749..>10325)
                     /locus_tag="VKQ52_21180"
     CDS             complement(8749..>10325)
                     /locus_tag="VKQ52_21180"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLZ89786.1"
                     /translation="RWIFHTIPYPGEAGYESWEDTAAYRHIGGANGWSGFTMDTGTGV
                     LFAPIGSASYDFYGGRRRGNDLFANCVVALDANTGKRLWHFQTVHHDIWDRDPPTPPV
                     LVTVKKDGKEIPAVAQVTKSGFVFLLDRKTGQPLSPVEERAAPTDSALPGEELSPTQP
                     IPTGMPAFARQSLTEADLNRLVPDSSFADLRKRLAGYRTGRLFIAPSRQGTVIFPGFD
                     GGAEWGGPAYDPQTGLLYVNANEMPWVLTMVDAKKAVAAVGGKAGAGAVQGKEATPVT
                     EAGGGRGRGVKVTVGTAAKALYATNCMVCHGANRQGGGNFPTLLGMDKKYNTEQFVTL
                     ISSGRRMMPAFRQLSDDEKQALAAFVLDQPAALGKRFVNASAKPIDPYFELPYTATGY
                     NKFLTREGYPAVAPPWGTLTAIDLSKGSIAWKDTLGDYPEFKAKGIHTGTENYGGAVV
                     TAGGVLFIAATSDAKIRAFNKRTGRLLWEADLPAAGFATPSVYAVDGREYVVIACGGG
                     KLHKKSGDSYVAFALP"
BASE COUNT         2445 a         3631 c         2246 g         2003 t
ORIGIN      
        1 ctctgggatg tcgtcctctg ggactggaca taaatcaaaa aaacgatgca acacccaacc
       61 atcgacatca aacgcgtata cgccgcccgc gaaaaagcag acggcatccg catcctcgtc
      121 gaccgcctct ggccgcgtgg gataaaaaag gaagaagccc acatcgaaga atgggcaaaa
      181 gacatcgccc catccgccga actccgcgaa tggtacggcc acgaccccga tcgctggccc
      241 gaattccaga aaaaatacaa ggctgaacta aaacacaacg aatccctgga cgccttcatc
      301 gaaaaatacc agcacgaaaa acacctcacc ctcatctacg ccacaaaaga cgaacaacac
      361 acccacgccc tcgtgctaaa ggactacctc gaccacgaat tctctcaccg ttgagccggc
      421 cccaccccaa caccgcgaac ccggccccgc taacccttcc ccctacccgc cgtcaccgca
      481 gccacctgct cccccgcatc acccaccggc ttcggcccca gctccgctgc cttcggcgcc
      541 acaacctccg cctgtggcac aacggtaacc gcctccggct gcggccgcaa cacactccgc
      601 aggatcgtct gcgggatcac cagccgcttc gggtcccgct tcaaataccc attgaacacc
      661 cctatcgcct tctgcaccag gaaatattgt aacaaggtct cgaaatccac ctccctcgca
      721 ggcaacagcg tcccggcccg ctccttatac gcccgcacaa accacccact cacataatgc
      781 gcccaaagcc ccgccatcgg taacatccgc tccacatccc cctcatgcaa ctgccgaccc
      841 tggaacaccc cctcaaacgc cacctcgtgg atactcagga tcatcgacgc caggtccacc
      901 gccggcgatc gccgcaaccg ccgctcacta tacgaaagcg acgggtcccc gctataatcg
      961 ctgatggcaa tatccttccc ggtcaacagt atctgcccaa gatgataatt cccatgtatc
     1021 ctgatcttct gcgtatccag cttcttcgca tagatcttct tcagcgtcgc cagcaactcg
     1081 ggccggtagg caacgatccg gtccagccgc tcccgcatat ccttcggcag cgacttcaca
     1141 ttccgcccaa ggttctggta ggtctcccgc acaagcgact gcatatagga aaaaagactg
     1201 cgctggtaat gcaacgagaa cggctccggc ttgaagtcct tgcccgtccc cgaagcaagc
     1261 gccagatgca tctccgccgt ccgcatcccg atcagccgca cctcctcggc cgcatgcgtc
     1321 cccagcaggg cctgcaattc gaccggcagc tcttcgaatg ccaccggccg ggccagcgtc
     1381 cccaaccgct cctgcggcaa cggcacactc ttttccgccg ccatgatccg ctcgatataa
     1441 ttattcaccc gctccaggaa gaacccatac ccgtccccat gattttcgat catctcctgc
     1501 aacaacccga gcacaataca tccccgctta tccttccact cgatactccc gataaaagca
     1561 ggcagatacg ggaaatgcgc ttccttcgac aggaaccgcg tcaactcctg gtcggaattg
     1621 accacccggt ccaccttgcg gtaaaacttc agaaagaaca ccccatcata cgcgatcgaa
     1681 gtattgtaca ggtcggaggt atggatcttc ggcgccgccc cggaaacacc tttcaaatat
     1741 tccttcaccc gcacactccc ctcaaaaacc accggtcccg ccgaatgccc cttatgcccc
     1801 gccgccaggc gctgcagcaa cgccatctgc cactcggaac taaacaacgc atccaccagc
     1861 atcccctcac cttcctccga ccgcacggtg gcgatcaatg cctcggggta cacctccccc
     1921 agcctcctcc ccgcctccac atccaccacc cataccggaa gctggtacat ccccggaacg
     1981 ccatcatcac ccatgatctc caacaggagc cagaacacct gccggccccc ggcctccaca
     2041 accgtatagt ccgccaccgt cacctggtag gtattccgct cccggggaat ataccacttc
     2101 gcccgcacca ggtagcgcgg caatatcgta ttctccaact gccgcctcgc cgtcccctcc
     2161 atcagccccg cccactgacg aagcagtaaa aggggtaaag gcttctcctc atccatctcc
     2221 ggatgcgcct tctccagcac aaaccactga aacgaatacg gcgataacgt aaagaaatat
     2281 tgcccatcct cccggatcac cgggaagcgg ttcctgctga acacatccac gggcacaaaa
     2341 cccttgtagg gaacaaggtc tacctccgcc gcctgcgaga acttcgacag gttgactaca
     2401 ataagcaggg tctcctcttc ataggtgcgc gtaaacgcca gaaccttggg gttctcggca
     2461 ttcacaaact tcaaattccc ccggccgaag gtcttgaact tctttcgcac ggcgatcagc
     2521 cgtttcatat accagaacag cgacgaggta ttcgcccgct gcgtctccac attcacggac
     2581 tcatagtgat actccgggtc gaggatcagc ggcagataca atttttgcgg attcgcggcg
     2641 gaaaaccccg cattccggtc gggcgaccac tgcatcggcg tccgcacccc atcccggtca
     2701 ccgagataga aattgtcgcc catcccgatc tcatcgccgt aataaataac aggagtcccg
     2761 ggcagggaaa aaagcaggga gttgagtagc tctatctttc gccggttatt atccagcagc
     2821 ggcgccagcc ggtgccggat gccaagattg atccgcgcct tcgggtcccg ggcatacacc
     2881 ttgtacatat agtcccgctc ctcatccgtc accatctcca gcgtcagctc atcatggtta
     2941 cgcaaaaaaa tagcccactg acaggtcaac gggatctccg gcgtctggtc aaagatatcg
     3001 gtgatggggt agtgatcctc catctgcaac gccatgaaca tcctcggcat caaagggaaa
     3061 tgataattca tctggcactc gtcgccattc ccgaaatacg aagccgaatc ctccggccac
     3121 atattcgcct ccgccaataa taccgtaccg ggaaaatgct cgtccaggtg ccgccgcagc
     3181 ctcttcaaaa aaacatgcgt ctccggcagg ttctctccat tcgtcccctc ccgctcgaat
     3241 agatacggca ccgcatccag ccggaaaccg tccacaccca tccggcacca gtgatcgagg
     3301 atccggaaca cctcttcctg caccgccggg ttgtcatagt tgagatcggg ctgatggtga
     3361 aaaaatcggt gccagtaata ctgctccgcc accgggtccc aggtccagtt ggaattctcg
     3421 aaatcctgga agatgatccg cacatccttg tattgatcgg gatcatccgt ccacacatag
     3481 aaactccgct ccttcgaccc ctttggcgcc tgtcgcgccc gctggaacca gacatgcgca
     3541 tcggatgtgt gattgatcac cagctcagtg atcaccttca aattgcgccg gtgcgcctcc
     3601 cgtaaaaaat gacggaatct cgaaacatcc ccgtaagacg ggttgatcgt ataatagtcg
     3661 gctatatcat agccatcatc ccgcaaaggc gaaggataaa aaggtaacag ccagatcgcc
     3721 gtcaccccaa ggtcctgcaa gtagtccaat ttctgtaaaa gcccctggaa atccccgaca
     3781 ccatccgcat ctccatcaca gaaagccttt atatgcaatt cataaatgat cgcatcctta
     3841 taccagtgta acgcatcgtc caatacattt gagccggcca tacccaaaca acggcaaatc
     3901 ccatgcacac ccccacgggg aaaaaattcc tcaccccccg atccttattt ctgtagtaaa
     3961 tgatccggcg aataagtgac aaatgtgcgt aattcatcat gcaataaaag cagatcatag
     4021 tgcaccgaag actgtgacac aagcacgggg ctgccaaaac gcttcgacat ctcccgctga
     4081 aagacccgct tccagaaagg ctgcgccgcc aacaccgcct ccgcatttat cagccgcacc
     4141 accaatacct tcatcttcct cctgcggtaa agctcgatgg cggtgatatc cgaccagggg
     4201 atcatcccac aggaaaaaat actccgccgg tcatcgatcc cttcggcgga aatgacaagc
     4261 cgcgcccgcc catcgaacaa ggtcttgata taatccacga gcaacaacgc cacccgcacc
     4321 agcaacatca ccagggaaat gccaaacaga aagccatact gggggccgaa atcctgcgtc
     4381 accatcaaca acacccacgc aacggtgatc cccgaataga taagcaatgc ggtggggacc
     4441 ctccgccgat tgaccggaat agaaagattc ataggccgta aagataacat tctctcccga
     4501 tccctcctcc cggccaggac acgcccgcct tgcaccgcca ctctttctcc cccctcctct
     4561 ccccgcaccc tcttctctcc cccctctccc gcaccctctt ctcttcccca ccctcctccc
     4621 gccggccgtt cacgccgtcc aataccgcac tttagccgca ttcaaagaaa ttttaatcca
     4681 ataaaaatat ttacacatat gtttctatct ggttaaaaac cacctcactc tgacgtctcc
     4741 gcgcgcactc aagaatacta tctaggcttt ttacgtacaa ccacttgtta atgaggatga
     4801 aatttttgta tttttatccg gatggcgaac ggaatatcct ttttaaagcc gtcaggtcta
     4861 ctcctcctgg cagccttttt tgttgttctc gtcccggcta acggcagccc cctcactact
     4921 cccatactgg ccggccagcg gttgtcgctg caaccctggc tcaccgtcct cgaagattcc
     4981 gccggttccg caaccgccgg cgacctcctc cgtcgtccga atgccttcgt accactcccc
     5041 tccctgcaaa agggtgaccc tacaagctat tactggctcc gcgccgacat ccgcacaaac
     5101 gatctctacg atccgggccg gatcctctcc ttcagccatc tcaccttcgt cgacgtatac
     5161 ctgtatgacg acaccggtct catcgttcat aaagtagccg gcgccttccg cccgcgcagc
     5221 aacctcgccg acggcgacgg caggcaatac gtcatgctgc ccctcgaaaa gaacaagacc
     5281 tacaccctcc tcatccgggt ccaccacaca aaacactacc agcccgtatt cgatttcgaa
     5341 atgatgccgg gccggcaata tttcaacacc ctcggcacaa aggaagcctt cgatgccgcc
     5401 ctcatgggcg ccgtcaccct ctttttcgtc tatacccttc tgtcatggat cgtcagccgc
     5461 ttccggccct atctctggct gctgctttcc atcggcggca tcgggttgta tgtgatcggc
     5521 atgaacggct attggatcga ctggttctcc cccgaacatc ccgcggcagg ctggctgctg
     5581 aacgtccact tcctccacct cggcatgctc ggcctctatt tactggtgac cgacttctgg
     5641 cacttaaaac aggatttccc gggcctctac gcctggggcc ggtggatacc cctcctcctt
     5701 ggcgtctcct ccctcgcctg tttcggcatc gactatttca ccggcaacta tagcctcgca
     5761 agcaacctca cctgcgcgga acatccgctg atactcgcct tcattctggc atccctcttt
     5821 atctgcggcc cacggctcag cgccgcccag cgctacctgg cctatggtat cgccctttgc
     5881 agcatcgccg gtgtcttcgt caccctaaac gcagtcatca accacgaacg gtcgctcccc
     5941 agcgtgagcc tcgtaggcga ctgcatcatc ctcaccgtct tcctcctctt ttccaccggg
     6001 ctaaaagaag agatgcgcca ccacgaactg gccaaacagg ttgccctgga agagctgaac
     6061 cgcctccagc aacaccagaa taccatcctc gaaaagaaag tggaagaacg caccgacgcg
     6121 ctgcggatat ccaacaaacg gctcctgaac cagaaacacc tgctcgcaga gagaaataca
     6181 aagatcgaac tcctgataaa tgagttgaac cacagggtaa agaacaacct ccagctcctc
     6241 tacagcctgc tcagcctgca actgcccctc gtcagggacg gcgtctcccg cgatatcctc
     6301 cagggcaata tcgggaagat acgggccatg atgctcgtga accaaaaact gttcaacttc
     6361 gaaaaaggcc gcggcatcgg cctctgcgag ttcatcaccg aactggccgt acacctgcaa
     6421 aagatctatg acacaaaaga aaagacccgg atcgtgcaga acatccccgc cggtctccgg
     6481 ttaagcgata aacacacact ctcctttggc ctcatactgt ccgagctgtt caccaacacc
     6541 ttcaaacacg cgttcaggga ccaacctgat ccttgtatct gcgtggaggc ggtagcggtg
     6601 accgaccagt tgctggaatt tcactactcc gacaacggcg ttggcatccc cgcaggcgaa
     6661 caacccgaaa agtttacgat gggcatcccc ctcatcaaag acctcacccg tcagatgaac
     6721 ggccagatga ccgtccgcgg ggacaaagga ctgagctatt ctttcactat tccagtttga
     6781 gtacctatgg taaggatact gatcattgaa gacgagatca tcatcgctcg ttttatcgaa
     6841 caccagctcc gggccaactt cgcctgcgag acccgcatcg ccgtctcggg aaaggaagcc
     6901 cgcacggcca tgaccgagat gcttccccat ctcctgttat gcgacatcca gctggaggac
     6961 gaactctccg gcatcgacct cgttactacc cttcgccggg gctatgcctt cgaggtcatc
     7021 tatatcacct cctaccagac ccactccatc atcgaaaaag ccgccgccac ccaccccgcc
     7081 aactatatca taaaacccat cgacgagacc cagctgttcg ccggggtaca actggtcatc
     7141 acccgcctcg acaccggtaa ccagaccggt aaacccctcc tccaggccga cagcctcttc
     7201 acagaaacag agctgcgcat ccttcgcctc atccgcgaga aaaaaaccac actggagatc
     7261 gccgacgccc tccacctcag ccgctatacc ataaaaaatc accgccataa catctgccgc
     7321 aaactcgaac tggccgatga gaacaatgcc ctcctcaaat gggtcgtcca gaacggacat
     7381 ctgctgaaat gatccgctat cggccaaaaa cacccgcgaa aaattgtttc attgcagccc
     7441 agctcgccgt atccgccttt gcattatacg ctatcggcag atggaacttc ttcccggcct
     7501 ccgtcgacgc cgggttcgta aacgcatgcg tggcgctatc ataaccgatg aaatgataat
     7561 cggccccgat ggaatccatc tgcttcctga atttggccac atcggcgggc gaaacgaact
     7621 tatcatcatt cccatggcag accaggatct tcgtccgcag caggtccttc cgcgccggcg
     7681 tcccgatcag gctcccgtga aaactaaccg cccccttcag atcatcgcca agccgcaccg
     7741 aattcaacaa caccccgccc ccaaaacaat acccgatggc cccgatattc gccgggtcga
     7801 cattgtcata tctccgcaat tgggcaatcg ctgaatcgat ccgtatcttg gccttcatcg
     7861 ggttcatata gaacggccgg gaatacgcgc ccgcactgtc gggattgtcg gccaccttgc
     7921 catccccata gatatccagc gccatcgcca catacccaag ccccgccagc atccgcgccc
     7981 gcatcttggg ataatcgtta agcccccacc actcggggat cacgatcacc gcgggccgct
     8041 tccccttcac cgagtcattg tacacgataa atgcatggag attggaggaa tccgcccgga
     8101 aagaaatatg ctcttccctg atacccgaag agtcggccgc cgtcgccggg gcagtggtcc
     8161 ccgtctgcct gggttcgtta gtacaggcgc ctgaaaacaa cagaaatccg cccaaaagcc
     8221 attgtggaaa ggaaaagaag ttacgcatgt gtagaatttt ggtcaaaggt acgcgaagcc
     8281 cgtggaatcc tatggggcct gacggatgac ggaatgattt tcctcaagac gaacatcggt
     8341 caccattccc atccgccgaa gatcatcccc gatcctctcc aggaggtcca ccggtgtctc
     8401 cgatcggtaa taagccgcct ctatcaccag gatcccggtg gaattgtctt ctttgatatc
     8461 cctggccctg gcctgccgca gaagggacag caccgattgc cggataccga catcattgcg
     8521 gcattccaca tgcatcaggt aagaatcttc gagagaggcg gtcggcaggg tcgtcgtaca
     8581 ggccgggata ctgagcagca gcactacgag gggtatatat acttttctca tcagcacggg
     8641 tttttatgtg gtaaagacca cacccgcaaa aaaattgtaa catccacccc accggaaacc
     8701 tcaaaaaagc ttctcaaacc ggtaaatcga cgacgcgaag ccgcctcact acggcaaggc
     8761 aaacgccacg taggaatccc ccgacttctt atgcagcttg ccgcccccac aggcgatgac
     8821 cacatactcc cgcccatcca ccgcatacac cgatggcgtc gcaaaacccg ccgcgggcag
     8881 atcggcctcc cataacaacc gccccgtccg cttattgaag gccctgatct tcgcatcact
     8941 cgtcgcggcg ataaacagga ctccgcccgc cgtcaccacc gcgccgccat agttctcggt
     9001 acccgtatgg atccccttcg ccttgaactc cgggtagtcg ccaagcgtat ccttccaggc
     9061 aatactcccc ttacttagat cgatggccgt aagcgtcccc cacggcggcg ccaccgccgg
     9121 gtatccctcc ctggtcaaaa acttattata acccgtagct gtatacggca attcaaagta
     9181 ggggtcgatg ggcttcgccg acgcattgac aaaacgtttc cccaacgccg cgggctgatc
     9241 cagcacaaag gcggccaacg cctgcttctc atcatcacta agctgcctga aggccggcat
     9301 catccgcctg cccgaagaga taagggtcac aaactgctcc gtgttatact tcttatccat
     9361 ccccagtaat gtcggaaaat ttccgccgcc ctgccggttg gccccatgac aaaccatgca
     9421 attcgtcgca tacaacgcct tcgccgccgt gcctaccgtc acttttactc cgcgccccct
     9481 gcctccaccc gcctccgtca ccggcgtcgc ttccttcccc tgcaccgctc ccgcaccggc
     9541 cttgccccca accgccgcca ccgccttctt cgcatccacc atcgtcaata cccacggcat
     9601 ctcattggca ttcacataca acaaccccgt ctgcggatcg tatgccggcc caccccactc
     9661 cgcaccgccg tcaaacccgg gaaagatcac cgttccctgt ctcgaaggcg cgataaaaag
     9721 ccggcccgtc cggtaacccg ccagccgctt ccgcaggtcg gcaaaagatg aatccggtac
     9781 cagcctgttc agatcggctt cggtcaggga ttgccgtgca aacgccggca tccccgtcgg
     9841 gatcggctgc gtgggcgaca actcctcacc cggcaacgca ctatccgtcg gcgccgcccg
     9901 ctcctccacc ggcgagagcg gctgccccgt cttccggtcc agcaggaata caaacccgct
     9961 cttggtcacc tgcgccacgg ccgggatctc cttcccgtct ttcttcaccg tcaccaacac
    10021 cggcggcgtc ggcgggtccc ggtcccagat atcatgatgc acggtctgga aatgccagag
    10081 ccgctttccc gtattcgcat ccagcgccac cacgcaattg gcaaaaagat cattgcccct
    10141 gcgcctgccg ccataaaaat cataggaagc cgacccgatc ggcgcaaaca atacccccgt
    10201 cccggtatcc atcgtaaaac ccgaccagcc attggcccca ccgatatgcc tgtacgcagc
    10261 cgtatcctcc cagctctcat accccgcctc ccccggatag ggaatggtat ggaagatcca
    10321 gcgca
//
LOCUS       DATGFG010001117         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1090983, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001117 DATGFG010000000
VERSION     DATGFG010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090983"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /locus_tag="VKQ52_21185"
     CDS             complement(<1..2158)
                     /locus_tag="VKQ52_21185"
                     /inference="COORDINATES: protein motif:HMM:NF040048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HLZ89787.1"
                     /translation="MRLLRIGFCFAACFLSLGSDAQKKTKPPEPPPPVAVKGGHLVYA
                     ADEKGNRIPDFSYCGYKGGDTSIPDVHIPIRVTVPQKDGDATFRLQAALDYVASLPAD
                     KNGIRGAVLLEKGVYEIDGALLLRHSGVVLRGSGMGEDGTILRAAGRDRRTLIRIAGI
                     NDRHTEKELTIADAYVPVNARHFRLAGANPFHPGDHILVHRPSTESWIKQLGMETFGG
                     GISALGWKAGQRDVYWDRTVTSIDGDGVTIDAPITTALDTAVGGGKVAGYQWPGRIEE
                     VGVENLRCVSDYDPQNPKDEAHSWMAITIENARDAWVRQVIVTHFAGSAVNILPTASR
                     VTVQDCKYLHPVSEIGGQRRNAFITDGGQTLFQRLYSERAFHDFAVGFCAPGPNAFVQ
                     CQSYYPYNISGAIDSWASGILFDVVNIDGQTLSYANREQDAQGAGWCAANSLFWNCTA
                     ARINCYRPPGAQNWSYGSWAQFGGDGFWSESNATLQPRSFFYAQLADRLGPEAAKRGD
                     IFPVENEASSSPTVEQAAALTAWSVKPRTQMTEWIDQAAQRDPIPVNAANVPAIDNLN
                     IKPPTPPQKAAPLQVVNGWLVRGNAILTGRRYYEPWWMGTVRPYGVEEAKPAITRWVP
                     GRTGTGLTDDLDALTDTMRDKHIVALDHNYGLWYDRRRDDHERIRRADGDVWPPFYEL
                     PFKRSGLDTAWDGLSKYDLTKYNPWYWDRLKRFADLA"
     gene            complement(2168..>3137)
                     /locus_tag="VKQ52_21190"
     CDS             complement(2168..>3137)
                     /locus_tag="VKQ52_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HLZ89788.1"
                     /translation="RHMFDPGDGSKEEKLGTVNITIQNCISAESLDTWNHAFGTTMGG
                     ENCMLVRNLWADNTGRNPSIGWNGVFNFINNVVFNWHHRSMDGGDYTSLFNIINNYFK
                     PGPVTPKEENVGHRILKPETGRSKLKDLHFGRAYVNGNIMDGYPEITKDNWNGGVQVQ
                     EEKDAGIYKDYMKVDKPFPMPPVTIMPAEEAYRFVLANVGATLPRRDAVDSRVVEQVR
                     TGKITYKEVNTDTLPQFHVRKLPKDSYKQGIVTEVFEVGGYPNYQGTPYKDSDNDGLP
                     DSYEISHGLNPKDPNDSAKPARDGSGYSNIEVWLNTLALKGEKPVK"
BASE COUNT          566 a          841 c          996 g          734 t
ORIGIN      
        1 cggcgagatc ggcgaagcgt ttgagacgat cccagtacca gggattgtac tttgtcagat
       61 cgtatttact aaggccgtcc catgcggtgt ccaggccgct acgtttgaat ggcaattcat
      121 aaaagggggg ccatacgtcg ccgtcggcgc ggcggatgcg ttcgtggtcg tcgcggcgcc
      181 ggtcgtacca gaggccgtaa ttatggtcca gggcgacgat gtgtttgtcg cgcatggtat
      241 cggtgagggc gtcgagatca tccgtcaatc ctgttccggt gcggccgggc acccagcggg
      301 tgatggcggg ctttgcctct tcgaccccgt aggggcgtac cgtacccatc caccagggtt
      361 cataatagcg gcggccggtg aggatggcgt tgccgcggac cagccaacca ttgaccacct
      421 gcaacggggc cgctttttgt ggaggtgttg gcggttttat gttcaggtta tcgatggcag
      481 gaacattagc ggcatttacc gggataggat cccgttgcgc tgcctggtcg atccactccg
      541 tcatctgggt gcggggtttt accgaccatg cggtgagggc ggcggcctgt tcgacggtag
      601 ggctgctcga cgcttcgttc tctaccggga agatatcgcc tctttttgcg gcttccggtc
      661 cgagacggtc agccagctgg gcgtagaaga aactgcgggg ctgtagggtg gcgttggatt
      721 cggaccaaaa gccgtcgccg ccgaattggg cccatgatcc ataggaccag ttctgtgctc
      781 ccggggggcg gtagcaattt atcctggccg cggtgcagtt ccagaacagg ctgttggcgg
      841 cgcaccagcc ggcgccctgg gcatcctgtt cgcggttggc gtagctcagc gtctggccgt
      901 cgatgttgac cacgtcgaag agaattccgg aggcccagct atcgatggct ccgcttatat
      961 tgtaggggta gtaagactga cattgtacga aggcatttgg tccgggcgcg caaaagccta
     1021 ccgcgaaatc atgaaaggcc cgttcggaat agagccgttg gaagagggtc tggccgccgt
     1081 ccgtgataaa ggcattgcga cgctgtcccc cgatctccga cacggggtgg agatatttac
     1141 aatcctgtac ggtgacacgg ctggccgtcg ggaggatgtt gaccgcggag ccggcgaagt
     1201 gggtcacgat gacctggcgt acccaggcgt cgcgggcatt ctctatcgtg atggccatcc
     1261 agctatgggc ttcgtctttt gggttctgcg ggtcgtagtc ggagacgcaa cgaaggtttt
     1321 ccacgcccac ctcttcgatg cggccgggcc actgatagcc cgctactttt ccgccgccaa
     1381 cggcggtatc caatgcggtg gtgatgggtg cgtcgatggt gacgccgtcg ccgtcgatgg
     1441 aggtgaccgt ccggtcccag taaacatccc gctggcctgc tttccagcca agggcggaga
     1501 tgccgccgcc gaaggtctcc atgcccagtt gttttatcca ggattccgta gacgggcggt
     1561 gtacaaggat gtggtcaccg gggtggaagg ggttcgctcc ggcgagccga aaatgccggg
     1621 cgttaacggg cacataggcg tcggcgatgg tcagctcttt ttcggtgtgc cgatcgttga
     1681 tgcccgcgat ccgtatcagc gtccggcgat cgcgaccggc cgcacggagg atggtaccgt
     1741 cctcgcccat gccgctgccc cggaggacaa cgccgctgtg gcgaaggagg agggcgccgt
     1801 cgatctcgta gacgcctttt tccaggagga cggcgccgcg gatgccgttc ttatcggctg
     1861 gcaggcttgc gacgtagtcc agtgcggcct gtagccgaaa cgtcgcgtca ccgtcctttt
     1921 gaggaacggt gacgcgtatg gggatatgaa catcaggaat ggacgtatca ccacctttgt
     1981 atccacaata agagaaatcc ggtatcctgt tgcccttttc atcagcggcg tagaccaggt
     2041 ggcccccctt taccgccacg ggtggcggcg gctcgggtgg ttttgtcttt ttttgtgcgt
     2101 ccgagccgag ggagagaaaa catgccgcga aacaaaaacc tattctgagt aaacgcattt
     2161 ccgaatctta ttttacgggc ttttcacctt ttagcgccag ggtattgagc cagacctcga
     2221 tattggaata gccgctgccg tccctggccg gtttggcgga atcgttgggg tctttcggat
     2281 tcaggccgtg actgatctca taggagtcgg gtaagccgtc gttatcggag tccttatagg
     2341 gcgtaccctg gtagttgggg tagccgccta cctcgaacac ctcggtaacg atgccctgct
     2401 tataagaatc tttcggcaat ttacgaacat ggaattgagg aagcgtatcc gtattaactt
     2461 ctttgtatgt tattttaccc gttcgtacct gttcgaccac gcgggaatcg acggcatcgc
     2521 ggcgcggcag ggttgcgcct acgttggcga gcacgaaccg gtaggcctct tcggccggca
     2581 tgatcgtcac cgggggcatg gggaagggct tgtccacctt catatagtcc ttgtatatac
     2641 cggcgtcctt ttcttcctgt acctggacgc cgccgttcca gttatccttt gtgatctcgg
     2701 gatagccgtc cataatatta ccgttgacat aagcgcggcc aaagtgaagg tcctttagtt
     2761 tactgcgacc ggtctcgggt ttcaggatac ggtggcctac gttctcctct ttgggtgtaa
     2821 cggggccggg tttgaaatag ttgttgatga tgttgaagag gctggtatag tcgccgccgt
     2881 ccatgctgcg atggtgccag ttgaacacga cattattgat aaaattgaag actccgttcc
     2941 agccgatgga ggggttacgg cccgtattgt ccgcccagag gttacgcacc agcatacagt
     3001 tctcgccgcc catcgtcgtg ccaaaggcat ggttccaggt gtcgagcgac tcggcggaga
     3061 tacagttctg gatggtgata ttcacggtac ccagtttctc ttcctttgag ccgtcgccgg
     3121 ggtcgaacat atggcgg
//
LOCUS       DATGFG010001118         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_409079, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001118 DATGFG010000000
VERSION     DATGFG010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409079"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VKQ52_21195"
     CDS             <1..1100
                     /locus_tag="VKQ52_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89789.1"
                     /translation="LFFSGPIPFMKGRADSDVPGTEERKGSGKWLERLEKKMTDLEER
                     NKMEVDKLLDLFKKNPDEALKYAIPLDSDGTSRGRGGDGVFSLVTRWLDLSPFGRSNP
                     AGGGGTVRMGDEFLRLNQQYQETARKLVKEGDYSKAAFIYLRLLKNYTLAAQTLEEGG
                     LYGEAASVYLRYNRDKTKAAECYEKGGMVQPAIELYKELGKEEKVGDLYRSIDRREEA
                     NHYFEKVAGDYTARGQYMKASVVVRNKMEDDVRAQALLMDGWRNKKDAVNCLTGYFSH
                     LRDEAALMDEMRRIYEKETDKDNRVAYLQVMKHTYQSREAVAGEARSIAYEIVAGNVA
                     KDPSIVAELQSFNRQDKNIISDILKFKHSRR"
     gene            1376..2062
                     /locus_tag="VKQ52_21200"
     CDS             1376..2062
                     /locus_tag="VKQ52_21200"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLZ89790.1"
                     /translation="MKPFHSYTNPALAVDLVVFGYSSNELSVLLLNRKEEPFKGGWTL
                     PGAFLQMDERFPDTCSRILRTKLGMDKLYLEQLYSFDDPERDPRGRAISIAYYALVNP
                     AKLKIAAGTMANDVQWINCRKLPALGFDHRQIFKTALQRLKGKILYFPVGFQLLDEQF
                     TMTELHELYECILDIPLDRRNFRRKILASEYILPTGTKREGLQNRHPELYVFNKKLKQ
                     DNFQLHIPLL"
     gene            2059..2721
                     /locus_tag="VKQ52_21205"
     CDS             2059..2721
                     /locus_tag="VKQ52_21205"
                     /inference="COORDINATES: protein motif:HMM:NF025489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HLZ89791.1"
                     /translation="MRERRWTKVKIESYPESQREIPAAGQYVIGHQTSDEIVVYQAYK
                     PSIAAYAVRHQFFGGDEYGYGRMSWIKPNFLWMMYRCGWAGKVNQERVLALWIKKSVL
                     ADILSDAVLSSFDPACYPDQQTWKRELEEKEVRLQWDPDHDPYGAKLTRRAIQLGLKG
                     RVLENFGKQQLLGIEDITDFVLEQKKQVDARRLDLLQVPVERVWDIPDVELKKKIGVS
                     EK"
     gene            2825..>3056
                     /locus_tag="VKQ52_21210"
     CDS             2825..>3056
                     /locus_tag="VKQ52_21210"
                     /inference="COORDINATES: protein motif:HMM:NF020302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HLZ89792.1"
                     /translation="MTYNKQWLIQKFPDAGVIPFLFFWGHQPSKDGSITKSCFSQWWV
                     APFEVEGVRYPTAEHWMMAGKAKLFGDEAALPK"
BASE COUNT          767 a          680 c          929 g          680 t
ORIGIN      
        1 gcctcttttt ctccggaccc atcccgttta tgaaagggcg tgccgatagt gacgtgccgg
       61 ggacggagga gaggaaaggg tcagggaagt ggctggagag gctggaaaag aagatgaccg
      121 acctggagga gcggaataag atggaggtgg acaaactgct ggacctgttc aaaaagaacc
      181 cggatgaggc gttgaaatac gccatcccgc tcgatagcga cggcaccagc cgggggcgtg
      241 gtggcgacgg tgtatttagc ctcgttacgc ggtggctcga cctgtccccg tttggccgtt
      301 ccaacccggc cggaggcggg ggaaccgtcc ggatgggcga tgagttcctg cggttgaacc
      361 agcaatacca ggaaacggcc aggaaactgg taaaggaagg agactatagc aaggcggctt
      421 ttatttatct ccggctattg aagaactata cgctcgccgc gcagacattg gaggagggag
      481 ggctctatgg cgaggcggct tccgtctatc tccgctataa tcgcgacaag acgaaggcgg
      541 cggaatgcta tgaaaagggt gggatggttc agccggcgat cgaattatac aaggagttgg
      601 gtaaggagga aaaagtgggg gatctttacc ggagtatcga ccggcgggag gaagccaatc
      661 attattttga gaaagttgcc ggtgactata ccgccagggg gcagtacatg aaggcatcgg
      721 tcgtcgtccg taacaagatg gaggacgatg tccgtgcgca ggccctcctg atggacggat
      781 ggcgaaataa aaaagacgcc gtcaattgcc tgacgggcta tttttcccat ctccgggacg
      841 aagcggccct gatggatgag atgcgccgca tctacgagaa ggaaacggat aaggacaacc
      901 gggtcgctta cctccaggtg atgaagcata cctatcaatc cagggaggca gtagccgggg
      961 aggcccggtc catcgcctac gagatcgttg ccgggaatgt ggcaaaggat ccttccatcg
     1021 tggccgaatt acagtcgttc aacaggcagg ataagaatat catcagcgat atcctgaaat
     1081 tcaaacattc caggcgctag atagacttag gatgtactaa gagttaattt gggttggggg
     1141 aaagttcccg gcgattcttc ctgtttatgg tcgcggatgg cgtattttcg tgcgactctg
     1201 attataatct tcttttcatt cttcagtcaa acaaacgaga agcgacccgg cctgacgccg
     1261 ggtcgctcat ttttaccgaa agacatcgaa atatcgccgc gggtagtttc ctgacggaaa
     1321 atttgctgat cccggttttt tgtcgttagt ttgcgtcaga ataacgcaaa ctaaaatgaa
     1381 accctttcat agctatacga atccggctct tgccgtcgac ctggtggtgt ttgggtatag
     1441 ttccaatgaa ctgtcggtat tgctgctcaa ccgtaaagag gagccattta aaggcggctg
     1501 gaccctgccg ggagcctttt tgcagatgga cgaacggttc ccggacacct gttcgcgtat
     1561 cctgcgcacc aagctcggaa tggacaaact ctacctcgag caattatatt cctttgacga
     1621 tccggagcgg gacccgcgcg gcagggcgat atcgatagcc tactatgcgc tggtgaaccc
     1681 ggcgaagctg aagatcgcgg ccggtacgat ggccaatgac gtacaatgga tcaattgccg
     1741 gaagctgccg gcgctgggtt tcgatcaccg gcagatcttt aaaacggcgt tgcagcggct
     1801 gaaggggaag atactctatt ttccggtggg cttccaactg ctggatgagc aattcacgat
     1861 gacggagctg catgaactgt acgagtgtat cctggatatt ccgctcgacc ggcggaactt
     1921 ccggcgcaag atcctggctt cggagtatat ccttcccacc ggcaccaagc gggaggggct
     1981 gcagaacagg catccggagt tatatgtgtt caataagaaa ctaaaacaag ataattttca
     2041 acttcatatc cctttattat gagagagcgg aggtggacga aggtcaaaat agaaagctat
     2101 cccgaatcgc aaagggagat ccccgcggcg gggcagtatg tgatcggtca tcagaccagc
     2161 gatgaaatag tggtatacca ggcgtacaag ccttcgatcg cggcgtatgc ggtgaggcat
     2221 cagttctttg gcggagatga atatgggtat ggacggatgt cctggatcaa gcccaatttt
     2281 ttgtggatga tgtaccgttg cggatgggcc gggaaagtga accaggaacg ggtattggcc
     2341 ttatggataa agaaatccgt attggcggat attttgtcgg acgcggtatt gtcgtccttt
     2401 gatcctgcct gttatccgga tcagcagacc tggaaacgcg aactggagga aaaggaggtc
     2461 cggctgcaat gggatcccga tcatgatccc tatggcgcga aactgacccg ccgggccatt
     2521 caattggggc tcaaaggaag ggtgctggaa aatttcggca agcagcaatt gctgggaatc
     2581 gaggatataa ccgattttgt gctggaacag aaaaagcagg tggatgcgcg gcggttggat
     2641 cttttgcagg tgcccgtcga acgggtatgg gatattccgg atgtggagct taaaaagaaa
     2701 attggggtgt cggaaaaatg aacacggtaa aattataagt ggttactgcg ttctttggtg
     2761 atgagttcac tatgccggcg aggcgagagt tgatcgaagt attattaaga ttcaaatgag
     2821 atctatgacc tacaacaaac aatggctgat ccaaaaattc cccgatgcgg gcgtcatccc
     2881 ctttcttttc ttttgggggc atcagccttc gaaggacgga agcattacca aaagttgctt
     2941 tagccagtgg tgggtggcac ctttcgaggt ggagggcgtc aggtatccaa cagcggagca
     3001 ctggatgatg gccgggaagg caaaattgtt tggcgatgag gcggcgctgc caaaga
//
LOCUS       DATGFG010001119         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_409262, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001119 DATGFG010000000
VERSION     DATGFG010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409262"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2045
                     /locus_tag="VKQ52_21215"
     CDS             <1..>2045
                     /locus_tag="VKQ52_21215"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HLZ89793.1"
                     /translation="ERTADMGIASIEWMSPAWVELLRFTLDEAASLGMTCDLIVGSGW
                     PFGAPWLAADERSRVRVVSAIRLEGPLDYEAVVADIIKDAYPAISAPFAGRVSKLISL
                     SLVPTPLNNLEDVKDIPVGDARKAYPISVPPGSHMLYGLVEFDGFSEVINGAPGADGP
                     VLNHYHKTAVEKYLHRMSDAIGPLTSHIRCFFTDSLELEGSNWCEDMEEEFLRRRGYA
                     LKPYLPFILYRTGSMGNNIDPEYGAKHGEVFRQLAGRVRFDFDLTKTELLRERFVEPF
                     LAWCRENKVLSRMQAYGRGYDPLDGSFGVDIPECESWIKPGLGTEMSEEDYRLGRAYT
                     MINKYISSAAHLQGKKIVSCEELTNTDRVFNETLELLKVGSDQSIISGVTHAVFHGFN
                     YSPREAAFPGWVRYGTYFNERNPWWPWLSHFTTYRARLTAIFQEMTMYAGIALLPPIA
                     DTWNKYGAQNTPFPTLIHPSYQPLIWEAIHKNGDGCDYVSEKTIQAGVLKKYHTLFLI
                     EVESLSPGTAEILYQFVAGGGRIFCIEAIPLTAESLLAPDAEVRDWVIRMEHFTDRFI
                     LLKKPARDYIGWYRNIQQDHSLKPYVVIRRPNPFVNQVRYRSKHAEAIFFINSNMEEG
                     YSLELSFAPEITAGKWPWLWDAVTGERRRLELSGDKLELHLGPADSKLIVFDKKKRG"
BASE COUNT          469 a          557 c          594 g          425 t
ORIGIN      
        1 ccgaaagaac cgcggacatg ggtatcgcct ccatcgaatg gatgagcccc gcgtgggtgg
       61 agctgctgcg gtttacgctg gacgaggccg cgtccctcgg gatgacctgc gatctgatcg
      121 tcggctcggg ttggccattc ggcgcgccct ggctggccgc tgacgagaga agtcgcgtga
      181 gggtcgtttc agcgatcagg ctggaaggtc cgctggacta cgaagcggtt gtggccgata
      241 tcataaagga cgcctatccc gctatttcgg cgccctttgc gggacgggtg tcgaaactga
      301 tttccctgtc tctcgtcccc acgcctttga acaacctgga agacgtaaaa gacattccgg
      361 tcggggacgc gcgaaaagcc taccctatat ccgttccccc cggcagccac atgttatacg
      421 gactcgtgga attcgatggc tttagcgagg tcatcaacgg ggcgccgggc gccgacgggc
      481 cggtattgaa ccattatcat aagacagcgg tcgaaaaata cctgcatcga atgtcggacg
      541 ccatcggccc gctgaccagc catatccgct gtttcttcac cgatagcctc gaactggagg
      601 ggtctaactg gtgcgaggac atggaagagg agttccttcg ccgaagagga tatgcactga
      661 agccgtattt accgtttatc ctgtacagaa caggcagtat ggggaataat atcgaccccg
      721 aatacggcgc aaaacacggc gaggtcttca ggcaattggc cggcagggtg cgttttgatt
      781 tcgacctgac aaaaaccgag cttttgcgcg aacgatttgt cgagcccttc ctcgcctggt
      841 gcagggaaaa caaggtactt tcccgcatgc aggcatatgg ccgcggctat gacccgctgg
      901 acggcagctt cggcgtcgat atccccgaat gcgagagctg gatcaaacct gggctcggaa
      961 cagagatgag cgaggaagac taccgcctgg gcagggccta tacgatgatc aacaaataca
     1021 tctcgtccgc ggctcattta caaggcaaga agatcgtttc ctgcgaggaa ttgaccaata
     1081 ccgaccgggt cttcaatgag accctcgagc tcctgaaagt gggatcggac cagagcataa
     1141 tatccggtgt cacgcacgcg gtttttcatg ggttcaatta ctctccgcgg gaagccgcat
     1201 tcccgggctg ggtcaggtac ggcacttatt ttaacgagcg aaatccctgg tggccctggc
     1261 tgtctcactt taccacctat cgggcaagac tgacggccat cttccaggaa atgaccatgt
     1321 acgccggtat cgcgttgctg ccgcccatcg cggacacctg gaacaagtat ggcgcacaga
     1381 acaccccgtt tcccacgctg atccatccca gctatcaacc gctcatctgg gaagcgatcc
     1441 acaaaaatgg ggatggttgt gattacgtct cggaaaaaac gatccaggcg ggtgttttaa
     1501 aaaaatacca cacgctgttc ctgatcgaag tggagagcct gagcccgggc acggcggaaa
     1561 tattatatca atttgtagcg ggcggaggcc gcatcttctg tatcgaagcc atcccattga
     1621 cggccgagag cctgctggct ccggatgccg aagtgcggga ttgggtgatc aggatggagc
     1681 attttacgga tcgcttcatc ttgctgaaaa agccggccag ggactatatc ggctggtacc
     1741 gtaacatcca gcaggaccat tcgctcaagc catatgttgt gatccgccgg cccaatccat
     1801 tcgtcaacca ggtccgctac aggtcgaaac acgcggaggc gatcttcttt atcaattcga
     1861 atatggagga ggggtatagc ctggaacttt ctttcgcgcc ggagatcaca gcgggcaaat
     1921 ggccctggct ctgggatgcg gtcacgggcg aacggcgccg gctggagcta tcgggggaca
     1981 aactggaact gcatctcggc cctgcggatt cgaaactgat cgtctttgac aaaaagaaaa
     2041 gaggg
//
LOCUS       DATGFG010001120         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_663066, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001120 DATGFG010000000
VERSION     DATGFG010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663066"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VKQ52_21220"
     CDS             <1..960
                     /locus_tag="VKQ52_21220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ89794.1"
                     /translation="IALSTCPPDLLLTPDKPDTLCQGADDSIKFKISSYVNNYTNWMM
                     QMSSDNGVTWTTPGLDTLGRPASGTVTPVYDGVSGLYLDTVTRYYRVPATSSITIYRL
                     IVASTLANLTTTGCFYTTNQPKYVYGVNCMIALPTTILSFRGQMHDGLGNLQWTTSNE
                     VENLSYSVMRSDDGVHFTAIATLPATGGSGGGAAYGFADPKPVGVQTYYRIDMVVSSA
                     RRNSGLVLLSNSNLPFEVRSVMNPFTDHINLELTAPGDGVATISLVDMYGRYLRRVRQ
                     SLTQGLNSFTLYDLAALPAGTYALQIQCNDQLVSKKLVKVINQ"
     gene            complement(1010..1711)
                     /locus_tag="VKQ52_21225"
     CDS             complement(1010..1711)
                     /locus_tag="VKQ52_21225"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HLZ89795.1"
                     /translation="MKARTIFLIVLLIIVADQSLKIWVKTHMPLSHPADFYRDPVSPY
                     DNGIKLLGDKAQIYFVENEGMAWGWKFGGSWGKMALTLFRLVAVVFGIYYIQTIIRKK
                     YHRGFIICAGLIFAGALGNLIDSMFYGLIFEHSTYDHVAKIFPTHGYTSFLHGRVVDM
                     LYFPIIKTHYPGWFPFVGGEEFEFFSPVFNLADASISVGVIAILLFQKRFFHKRPGAG
                     AHSLDGQSKVDAVQA"
     gene            complement(1771..>2353)
                     /gene="alr"
                     /locus_tag="VKQ52_21230"
     CDS             complement(1771..>2353)
                     /gene="alr"
                     /locus_tag="VKQ52_21230"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLZ89796.1"
                     /translation="TTRQAALYKSTAHRLEQTLGYSFLRHIANSAGIIRHPELQLDMV
                     RLGIGLYGIDPAGSDRLELQEVSTLKTTIAQIKYLHAGDTIGYNRRGIAAAGTVTATV
                     RIGYADGYSRSLGNGVGKMWINGRLAPTIGIVSMDMTMIDITGIPDVKEGDEVIVFGK
                     ELSVKQLAEWSKTIPYEILTSVSQRVKRIYFEE"
BASE COUNT          503 a          671 c          640 g          539 t
ORIGIN      
        1 atcgcgttgt ccacctgtcc gcccgatctg ttgctgacgc ccgataagcc cgacacgctt
       61 tgccagggcg ccgacgactc catcaaattc aagatctctt cctacgtcaa taactatacg
      121 aactggatga tgcaaatgag cagcgataac ggcgtcacct ggactacgcc cggtctcgac
      181 acgcttggcc gccccgcgag cgggaccgtg accccggtgt atgacggagt ttcggggctc
      241 tatctcgata cggtgacccg ctactaccgg gttccggcga ccagcagtat tacgatctac
      301 cggctcattg tagccagtac cctggcgaac ctgacgacca cgggctgctt ctatacgact
      361 aaccaaccca aatatgtata tggcgtcaac tgtatgatcg ccctgcccac taccatcctg
      421 tccttccgag ggcagatgca tgacggcctt ggcaatctgc aatggacgac gtcgaacgaa
      481 gtggagaatc tttcctattc ggtgatgcgc agtgatgacg gcgtgcactt cacggcgata
      541 gctacccttc ccgcaacggg gggtagtggt ggtggggccg cttatggctt cgccgacccc
      601 aagccggtgg gtgttcaaac ctattaccgc atcgacatgg tcgtttcatc ggcccgtcgt
      661 aacagtggtc ttgtactcct tagcaacagc aacctcccat tcgaggtgcg atcggtgatg
      721 aatcctttca ccgaccatat caacctcgag ctgaccgcgc ctggtgatgg tgttgcaacc
      781 atcagcctgg tcgacatgta cgggcggtat ctccgccgcg tgcgccagtc gctgacgcag
      841 ggcctgaaca gctttacgct ctatgatctt gcggcgttgc ccgcgggaac ctatgccctg
      901 cagatccagt gcaatgatca gttggtaagt aagaaactcg ttaaggtgat taaccagtaa
      961 aacgcgcagt aattatcctt tgtgaagggc cgctccggtg gccctttttt tatgcctgta
     1021 ccgcgtctac cttggactgc ccgtcgagcg aatgagcgcc ggcgccgggg cgtttgtgga
     1081 aaaagcgttt ttggaagaga aggatggcga tcacgcccac cgagatggag gcatccgcca
     1141 gattgaagac ggggctgaag aactcgaact cctcgccgcc gacaaagggg aaccaacccg
     1201 ggtagtgcgt ttttatgatg gggaagtaga gcatgtccac cacccttcca tggaggaaac
     1261 tggtgtagcc gtgcgtcggg aagatcttcg ccacatggtc gtaggtacta tgctcgaaga
     1321 tgaggccata gaacatgcta tcgatgagat tgccgagcgc gcccgcgaag atgagtcccg
     1381 cgcaaatgat aaagccgcga tgatatttct tccggatgat cgtctggata tagtagatgc
     1441 cgaagacgac ggcgacgagc cggaaaagcg tcagcgccat cttaccccag ctgccgccaa
     1501 atttccagcc ccaggccatt ccttcgttct ccacgaaata gatctgcgct ttgtcgccca
     1561 gtaatttgat gccattatcg taaggggaaa ccgggtctcg gtagaaatcc gccggatggc
     1621 tcagcggcat atgtgtcttt acccagatct tcagcgactg atccgccaca atgatcaaca
     1681 atacaattaa aaagatggtt ctggccttca caatacaata aattagctcc aaatataatc
     1741 aatcctaccc attgaattcc ccctctcatc ctactcttca aaatagatcc ttttgacccg
     1801 ctgtgatacg ctcgtgagga tctcgtaggg tatggttttt gaccattcgg ctaactgctt
     1861 gacagatagc tctttgccga atacgatcac ttcatcgcct tcttttacgt cggggatgcc
     1921 ggtgatatcg atcatcgtca tgtccatgct cacgatgccg atggtgggtg cgagccgtcc
     1981 gttgatccac atcttcccca cgccgttccc gaggctccgg gagtagccat ccgcgtagcc
     2041 gatgcgtacg gtggcggtga ctgttcccgc cgccgctatc cctctgcggt tatacccgat
     2101 cgtgtccccg gcgtgcaaat actttatctg ggcgatggtc gtctttagtg tcgacacttc
     2161 ctgaagttcg aggcggtcgg agcccgcagg atcgatgccg tagagcccga tgcccaggcg
     2221 caccatatcc agttgcagct ccgggtgccg gatgatgccc gccgagttgg cgatatgccg
     2281 gaggaaggaa taccccaggg tctgttcgag gcggtgggcg gttgacttat acagcgctgc
     2341 ctgccgggtg gtg
//
LOCUS       DATGFG010001121         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1552171, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001121 DATGFG010000000
VERSION     DATGFG010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552171"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VKQ52_21235"
     CDS             complement(<1..429)
                     /locus_tag="VKQ52_21235"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HLZ89797.1"
                     /translation="MKKGNSPLILLLLVAMAAGIAACKKVISVDLNNAAPQIVIEGEI
                     TNARGPYQVKISKTVNFSASNVYPPVTNATVTITDSTSGISEQLIGSDSGIYKTRNIV
                     GAARHTYALNVMVDGKQYSAVSTMPVQVLLDSVTFALNFDV"
     gene            complement(433..2766)
                     /locus_tag="VKQ52_21240"
     CDS             complement(433..2766)
                     /locus_tag="VKQ52_21240"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLZ89798.1"
                     /translation="MKIRFRRLILLLMLTLAVTAATAQSRYTVNGTVRDKNSGEVLIG
                     ATVSLLEIPRSGIVSNAYGFYSIGASPGHYNLLVTFSGYQSDTLAIDLDRNLLLPVEL
                     TTASSQLTAVVVSARKKNDNVTRPLMGVQKLTTSEIKNIPVLFGEKDVMKTLQLLPGI
                     QFAADGNSGFYVRGGGADQNLILLDEATVYNPSHLLGFFSTFNSDAIKDITIYKGGMP
                     AEYGGRLSSVVDIKMNDGSNKDYHYGGGIGLISTRLNAEGPIVKDRGSFSISGRRTYA
                     DLFLKLSSDSSVSNNSLYFYDLNIKANYTFDEKNRVYLSGYFGRDNLSFGNTFGIDYG
                     NATGTARWNHVFNSRLFSNTSLIYSKYSYKIRINSNNNNIGFSSNIKDINLKEDLQYY
                     IDADNKINFGLSAIHHTIAPGIISASQSSSFNSLTLQNKYSLENAIYVSHEWSPTDHL
                     HFNYGLRASSFMALGPGNFYTYDQNGNPRDTIQYNSGSIVKTYTNLEPRLSLSVQLNE
                     AASVKLSYNRNTQVLHLLSNSTSANPTDLWIPSSNNVKPEISDQESIGFFTNSRDNRY
                     EFSVETYYKTMQNQIDYKNGAQLIANENVESQLVFGKGRAYGLELFGKKKVGRLTGWI
                     SYTLSRTELKISGINQDNWYPAKQDRTHDISVVGIYQAGPKWTFSATWVYYTGNAVTF
                     PSGKYQVDGQTAFYYTERNGYRMPAYHRMDVAATLLGKKKKKFESSWTFSIYNLYGRE
                     NAYSIIFQTDPNDPAKTQALQYALFRFVPAVTYNFKF"
     gene            complement(2793..>3242)
                     /locus_tag="VKQ52_21245"
     CDS             complement(2793..>3242)
                     /locus_tag="VKQ52_21245"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HLZ89799.1"
                     /translation="GISMKKEKNYQAAFKPHLQLIKGLPALLEKTEKQGIKMAIGSAA
                     IPFNIDFVLDNLHLRHYFNAIVSADEVAISKPHPETFLLAAQLLNTAPNDCLVFEDAP
                     KGVEAALRAEMPCIVLTTMHEKEEFRRYPNVLRYIADYTDPSLDELF"
BASE COUNT          652 a          727 c         1033 g          830 t
ORIGIN      
        1 aacgtcgaag ttgagggcaa aggtcaccga atcgagcaag acctggacgg gcattgtcga
       61 gacggcgctg tattgttttc cgtccaccat tacgttcagg gcataggtat gccgcgcggc
      121 tccgacgatg ttccgggtct tgtaaattcc cgaatccgac ccgatgagtt gttccgatat
      181 cccggaggta ctatcggtga ttgttacggt ggcattggtg acgggcgggt agacgttgct
      241 ggccgagaaa ttcaccgtct tgcttatctt aacctggtag ggtcccctgg cgttggtaat
      301 ctccccttcg atgacgatct gcggcgcggc gttgttgaga tcgacgctga tcaccttctt
      361 gcaggcggcg atcccggcgg ccatagcgac caggaggaga aggataagag ggctgtttcc
      421 ttttttcatc gatcaaaatt taaagttata ggtcaccgcg ggtacgaatc ggaacagggc
      481 gtattggagg gcctgtgtct ttgccggatc gttgggatcg gtctggaaga tgatgctata
      541 ggcgttctcc cggccataga ggttatagat ggagaaggtc cagctgcttt cgaatttctt
      601 tttctttttt cccagcaggg tggcggccac gtccatccgg tgataggcgg gcatccggta
      661 gccgttgcgt tcggtataat aaaaggccgt ttgtccatcg acctggtatt tgccgctggg
      721 gaaggtgacg gcgttgccgg tgtagtagac ccaggttgcc gagaaggtcc atttggggcc
      781 ggcctggtag atacccacga cagagatatc atgggtccgg tcctgtttgg cgggatacca
      841 gttgtcctgg ttgatgccgg agatcttcag ttcggtgcgg gataaggtat agctgatcca
      901 gccggtaagc cggccgacct ttttctttcc gaagagctca aggccgtagg cccggccctt
      961 gccgaagacg agctgcgatt cgacgttctc attggcgatg agttgggcgc cgttcttata
     1021 gtcgatctgg ttctgcatcg ttttatagta ggtctcgacg gagaactcgt agcggttgtc
     1081 gcgggagttc gtaaagaacc cgatcgactc ctgatcggat atctctggtt ttacgttgtt
     1141 gctgctggga atccagagat cggtagggtt ggccgaggtg gagttggaca ggaggtgcaa
     1201 cacctgggta ttccggttgt aggaaagttt taccgaggcc gcttcattca gctgaacgct
     1261 caacgacagc cgtggttcca gattggtgta tgtctttacg atgctgccgg agttgtactg
     1321 tatcgtatcc ctggggttac cattttgatc ataggtgtag aagttgcccg ggcccagcgc
     1381 catgaaggag gatgcccgca gtccataatt gaagtggagg tgatccgtgg gggaccattc
     1441 gtgtgagaca tagatcgcat tttcgaggct gtatttgttt tgcagcgtca gcgaattgaa
     1501 actggaggac tgggacgcgg atatgatgcc gggggcgatc gtatggtgga tcgcgctcag
     1561 gccaaagttg atcttgttgt cggcgtcgat atagtactgc agatcttcct tcaggttgat
     1621 gtccttgata ttggagctga atccgatgtt attgttattg gaattgatcc tgatcttata
     1681 gctgtacttg ctatagatga gcgaggtgtt ggagaagagg cggctgttga agacatgatt
     1741 ccagcgcgcc gtgccggtgg cattaccata gtcgatacca aaggtgttgc caaagctgag
     1801 gttgtcgcgg ccgaaatacc cggagaggta gacccggttc ttctcgtcga atgtatagtt
     1861 tgcttttatg ttgaggtcat agaaatagag gctgttgttg ctgacggaag agtcggagga
     1921 cagcttgagg aagaggtcgg cataggtacg gcggccgctg atcgagaacg agccccggtc
     1981 ttttacgatc gggccttccg cgttgagccg cgtggagatc aggccgatac cgccgccata
     2041 gtggtagtcc ttgttactcc cgtcattcat ttttatatct acgacggagg aaagccggcc
     2101 gccgtattcc gccggcatgc cgcccttgta tatggtgata tcctttatgg cgtcggagtt
     2161 gaaggttgaa aaaaagccca gcaggtggga ggggttatag acagtggctt cgtcgaggag
     2221 gatgaggttc tggtccgctc ccccaccccg gacgtaaaaa ccgctgttac cgtcggcggc
     2281 aaactggatg cccggcagca gctggagggt cttcatgaca tccttttcac caaacagcac
     2341 ggggatgttc tttatctcgc tggtggtgag tttctgaaca cccatcaggg gcctggtgac
     2401 gttatcgttc tttttccggg cgctcaccac caccgccgtc aactggctgg aagcggtggt
     2461 aagctcaacg ggaagcagga ggttcctgtc gagatcgatg gccagggtat cggactggta
     2521 gccggaaaaa gtcaccagga ggttgtagtg accgggggag gcgccgatgg aatagaagcc
     2581 gtaggcattg ctgacgatgc cggagcgggg tatttcgagc aggctgacgg tagcgccgat
     2641 gaggacttcc ccggaattct tatcccggac ggtgccgttg acggtataac gggattgagc
     2701 cgttgcggcc gttaccgcga gcgtcaacat aagcagtaag atgagcctgc ggaaacggat
     2761 tttcatggat agatctggtt gtctgtttcg ctttagaata attcgtcaag tgacggatcc
     2821 gtatagtctg caatatagcg caggacgttg ggataacggc ggaattcctc cttttcgtgc
     2881 atggtagtca ggacgatgca gggcatttcc gcgcgcaagg cggcttcgac gccttttgga
     2941 gcgtcttcga agacgaggca gtcgttgggg gcggtgttca ggagttgggc cgccagcagg
     3001 aaggtttccg ggtggggttt gctgatggcg acctcatccg cgctgacgat ggcgttgaaa
     3061 taatggcgga ggtggaggtt atcgagtacg aaatcgatat taaaggggat ggctgccgag
     3121 ccgatggcca ttttgatacc ttgcttttcg gtcttttcca gcagcgcggg gagacctttt
     3181 atgagttgga gatgaggttt gaaggccgcc tggtagtttt tttctttttt catggagatc
     3241 cc
//
LOCUS       DATGFG010001122         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1109652, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001122 DATGFG010000000
VERSION     DATGFG010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109652"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VKQ52_21250"
     CDS             <1..763
                     /locus_tag="VKQ52_21250"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89800.1"
                     /translation="QSFFEADLQWMNRAHDAGQAIACIRLAQTAGFDNISIDLIYGGP
                     TLPDSHWKENVARAIGMGIPHLSCYALTVEPRTALDTFIRRHQVADVSQDDQARQFLL
                     LMDWMAAAGYEHYEISNFSLPGYRSRHNSAYWQGKKYLGLGASAHSYNGVSREWNVAN
                     NARYIAAPGLDTEKEMLTPVQQVNEYIMIALRTLEGIDLSVLAGRFGAGRAQELRGRA
                     EKYLRETKLSLVAERLQLTTEGKLLADGIAADLFF"
     gene            complement(953..1999)
                     /locus_tag="VKQ52_21255"
     CDS             complement(953..1999)
                     /locus_tag="VKQ52_21255"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HLZ89801.1"
                     /translation="MGMRFGVIGSGSWATALVKILTDNRQPVGWWIRNESSIRHILAR
                     HHNPQYLPSVYFDTDLLTMSDRPAEIVAAADCLVMAVPSAYTAATLAGLDRKVLHGKK
                     VLSAIKGILPEQNLLLNEWLSREFDFPIADYYTVMGPCHAEEVAAEKLSYLTFSGLDE
                     TSTRELASYFTTDYLNTVINNDVYGVQYAAVLKNIYALGAGIAHGLEYGDNFLSVLIA
                     NCADEMAGFLQKVGIRHSGVVGVHEEEDPVSHRRSPNYASSVYLGDLLVTCYSLYSRN
                     RTFGNMIGKGYSVQSAQLELNMVAEGYNASKCIYLINKDIKAEMPIAGMIYRILWENV
                     KAGSGFKQIEPYLV"
     gene            complement(2003..3394)
                     /locus_tag="VKQ52_21260"
     CDS             complement(2003..3394)
                     /locus_tag="VKQ52_21260"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ89802.1"
                     /translation="MNKQALRIIIILVAVIVLLVVLKQAGAFGKDEGVRVSSEKVIRR
                     NITEVVPASGKIYPEKEVKISPDVSGEVVELGVSQEGDSVHKGQVLARVYADILTNQR
                     DQAAAQMSQQQALVSNIQEQLPGLKASMETSKKALDREKQLLDEKVVSLAEYETAENT
                     YRTAQANYQAALQSVRGNQAGATSARYNLDVAAKNLSRSTVVSPIEGVVSLLSIKKGE
                     RVVGNSMMAGTEMMRVADMSRIEAIVDVGENDIPKVRLGDSALITVDAYNNRKFKGLV
                     TQIASSVTNAAGIATTTVSTNDVTNYKVHIRLSPDSYKDLIDPAHPRNFPFRPGMSAS
                     ADIQTNTHFNALSVPINAVAVREKNTDNAVVGNTDESGSDNKPDAKAAVSGAGSQDLD
                     EVVFLRQPGDTVRKVKVRTDIQDINYIEVVNGVKEGDEVITGPYSVVSKTMKGGTRVK
                     VVPKEKLFEEKKN"
     gene            complement(3399..>4040)
                     /locus_tag="VKQ52_21265"
     CDS             complement(3399..>4040)
                     /locus_tag="VKQ52_21265"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ89803.1"
                     /translation="RKTIESNRGAMYPTLSVVGSLGAGWISNFPEPDLSKPLKYTGTD
                     TLYTLRAVGANSVFVQDNYAYSTKQKPFFPQLHQTFNQFIGVNLNIPILNNGTLRINY
                     AKSKLTLRNQELQRDQDNLTLKQNIYQAYTAAITALQKFEANKITVTATQKSFDYAQK
                     RYNIGMLNTIDLLTNQNNFFNARINLLSSQFDYVFKMKVLEYYKGLGIKLTKE"
BASE COUNT          841 a         1176 c         1078 g          945 t
ORIGIN      
        1 acagtctttt ttcgaagccg acctgcaatg gatgaaccgg gcccacgacg cgggacaggc
       61 aatagcatgt atccgtctgg cgcagacggc tggctttgac aatatcagca tcgatctcat
      121 ctatggcggc ccaaccctcc ccgatagcca ctggaaggag aacgtagcgc gcgctatcgg
      181 tatgggcatc ccacacctgt cctgctatgc gctgaccgtg gagccgcgca cggccctcga
      241 cacctttatc cgtcgccacc aggtggccga tgtcagccag gatgaccagg cccggcaatt
      301 cctgttgctg atggactgga tggccgccgc cggctacgaa cattacgaga tctccaattt
      361 ctccctgccc ggataccgga gccggcacaa ctccgcctac tggcagggca aaaaatacct
      421 tggcctcggt gcatccgcac actcttacaa cggagtgtcg agggaatgga acgtcgccaa
      481 caacgcccgc tatatcgccg cgcccgggct cgacaccgaa aaagagatgc ttaccccggt
      541 acagcaggtg aatgaataca tcatgatcgc gctcaggacc ctggaaggta tcgatctttc
      601 ggtcctggcc ggacgctttg gggccgggcg cgcacaggaa ttgcgcggtc gcgcggaaaa
      661 atatctccgg gagacaaagc tcagcctcgt ggcggagcgg ctgcaattga caacggaagg
      721 taaattattg gcggatggca tcgccgcgga tctgttcttc taaccggatg acatccggct
      781 tcagacaacg cagatcaatg atgccggtgg cgggacttca aatagtcgat cccctgggct
      841 gccgacagga tgagcaccat cgcgatgatg gccatagcac caaaaccctg aaaggagaaa
      901 ggcatatatt tatcgtttta tggaacccgg aattccccgg atccctttct tcttagacaa
      961 gatacggctc aatttgttta aatccactac cggctttcac attttcccat aaaatccggt
     1021 agatcatccc cgctatcggc atttcggcct ttatatcctt gttgatcaaa tagatgcatt
     1081 tacttgcatt atatccctcc gcgaccatgt tcaactccag ctgggccgac tgtaccgaat
     1141 atcccttccc gatcatattc ccgaaggtgc ggttgcggct atagagcgaa tagcaggtaa
     1201 ccagcaggtc cccaagataa accgaggagg catagttggg gcttcgccgg tgggaaacag
     1261 ggtcttcctc ctcatgaacc cccacgaccc cgctatgcct gatccccacc ttttgcagaa
     1321 agcccgccat ttcatccgca cagttggcga tgagcacgct gagaaaatta tcgccatatt
     1381 ccagtccatg cgcgataccc gccccaaggg catagatatt cttcaggacg gcggcatact
     1441 gcaccccgta tacatcgtta ttgatcactg tattcaggta atccgtcgta aaataggacg
     1501 ccagttcgcg ggtggaagtt tcatccagac ccgaaaaagt aagataggag agcttttccg
     1561 ccgccacctc ttcggcatgg cagggaccca tcacggtgta atagtcggcg atggggaaat
     1621 cgaattcgcg ggaaagccat tcattcagca gcaggttctg ctccggcagt atgcccttta
     1681 tcgccgatag gaccttcttc ccgtgtaaga ctttccggtc cagaccggcc agcgtggccg
     1741 cggtataagc cgaaggaacg gccatgacaa gacagtcggc tgcagccacg atctccgccg
     1801 gcctgtcact catcgtcagc aggtccgtat cgaaatagac ggagggaaga tattgcggat
     1861 tatgatgtct ggccaggata tgacggatgc tcgactcgtt cctgatccac cagcccaccg
     1921 gctgccggtt atccgtaagg atctttacca gcgccgtagc ccagcttccg ctacctatca
     1981 ccccgaaacg catacccatc ggttaatttt tcttttcttc gaacagtttt tctttcggca
     2041 ctactttcac cctcgtccca cccttcatgg tcttgctcac gacgctgtac ggacccgtga
     2101 tcacctcgtc tccttcctta accccgttca ccacttcgat gtaattgata tcctggatat
     2161 ccgtacgcac cttcactttc cggacggtat cgcccggctg ccgcaggaag accacttcgt
     2221 cgaggtcctg cgagccggcg ccggagacgg cggccttggc atcgggcttg ttgtcgctgc
     2281 ccgactcatc cgtattgccc accacggcat tgtccgtatt cttttcccgg acggcgacgg
     2341 cattgatcgg cacggagagc gcgttgaaat gggtgttggt ctggatatcc gcgctggcgc
     2401 tcatccccgg ccggaacggg aagttccggg gatgcgcagg atcgataagg tccttatagg
     2461 aatccggcga cagccggata tgaaccttgt agttggtgac atcgttggtc gacacggtgg
     2521 tcgtggctat gccggcggca ttggtaacgc tgctggctat ctgtgtgacg aggcccttga
     2581 acttccggtt attgtaggcg tccaccgtga taagcgccga atcgcccagc ctcaccttgg
     2641 gaatatcgtt ctcgccgaca tcgacaatgg cctcgatcct gctcatatcg gctacccgca
     2701 tcatttcggt gcccgccatc atcgagttgc cgaccacccg ctcgcctttt ttgatgctca
     2761 gcaaggagac gacgccctcg atgggggaga caaccgtact ccggctcagg ttcttggcgg
     2821 cgacatcgag gttgtaccgg gcactggttg cccccgcctg gttcccgcga acactttgca
     2881 gcgccgcctg gtagttggcc tgcgccgtac gataggtgtt ctcggcggtc tcatattcgg
     2941 caagcgagac cactttctca tcgagcagct gcttctcgcg gtcgagggcc ttcttcgacg
     3001 tctccatcga ggccttcaga ccgggcaact gctcctgtat attggaaaca agcgcctgtt
     3061 gctggctcat ctgcgccgcg gcctggtccc gctggttggt aaggatatcc gcgtacaccc
     3121 tggccagcac ctgccccttg tgcacgctgt ccccttcctg cgaaacaccc agttccacca
     3181 cttccccgga aacatcgggg ctgatcttta cctccttctc gggatagatc ttgccgctgg
     3241 caggaacgac ctccgtgata ttgcggcgga tgaccttttc ggaagacacc ctgacccctt
     3301 cgtcttttcc aaacgctccg gcctgtttca gcacgacgag cagcacgatc actgcgacaa
     3361 gaatgataat gatacgcagg gcttgtttat tcatgaggtt attcttttgt cagttttatt
     3421 cccagtccct tgtaatattc cagcaccttc atcttgaaca catagtcgaa ctgactgcta
     3481 aggagattga tccgggcgtt gaaaaaattg ttttgattgg tcagcaggtc gatggtattc
     3541 aacatgccga tattgtagcg cttttgcgca tagtcaaagc tcttctgggt ggcggtgacg
     3601 gtgatcttgt tggcctcgaa tttctgcagc gccgtgatgg cggccgtata ggcctggtag
     3661 atattctgct tgagcgtaag gttatcctgg tcacgctgca gctcctggtt gcgcagtgtc
     3721 agtttcgact tcgcgtaatt gatccgcagg gtgccgttgt tgaggatggg gatattgagg
     3781 ttgaccccga tgaactggtt gaaggtttga tgcagttggg gaaagaaggg cttctgcttc
     3841 gtggaataag catagttatc ctgtacgaaa acagaattgg cgcccaccgc ccgcagggta
     3901 tacaaggtgt ccgtgccggt atacttgagc ggtttcgaaa gatccggctc ggggaaattg
     3961 ctgatccacc cggcgccaag ggacccgacg accgaaaggg tcgggtacat cgcccccctg
     4021 ttcgattcga tggtcttgcg
//
LOCUS       DATGFG010001123         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_68657, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001123 DATGFG010000000
VERSION     DATGFG010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68657"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2255
                     /locus_tag="VKQ52_21270"
     CDS             <1..2255
                     /locus_tag="VKQ52_21270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLZ89804.1"
                     /translation="FGTSLLNIPTQVDLGNFSQALPLTPEGIQLIRVNGKWIAIITGG
                     NTYGANNESRVIKMDFGNSLANTPTATNWGNIGALNFPDDIFIANDGGTYIGYAINVL
                     DNTLTRWNFGTDFSGVPTGTNLGNPGGTVDWPVGSGYFNYNGKWYGFIANRNSNSLTR
                     LDFGTSLANTPTAVNIGNPGGLLNYPRDFAIFATCGEIYGFVPNEGTNALVKLDFGSD
                     PTNPSPTATSLGNIGNLDYPHSITNLFRAGNDVYAFICNAYSNTLSRMRFAGCQDIPG
                     STAAAPAPVTYHTPGTYTISLLVDVGLPTQTSYCQKITVLASPLGTIQGDTVCFGSSP
                     ALNFAGTGTGPFDISFSDGNQTYTQGGLPVQSSIPLPNTLTAPGSAEFVLQKVSDGTG
                     CSTSLNQSTNILIRPAPQSGITGTTICGSDSALISLQAGAGVPPFEVQISNGSTSFVV
                     NGVKPGAAFPVAFPGMNAAANFSLMEVKDSVGCPEETGFAPGVTNVVPLPAPAVVFPS
                     LGAVCYDKEPFVLGTASETTGLAGTGVYSGSGIATGGTFSPAAAGPGAHEIKYTYVAA
                     DGCTAVDSSVITVNALPKASTVPLITACEGVPVRLTASGGNTYLWSPAGDLSDPTSQT
                     PVATVDTTTMFIVEVTDSNGCSATDSILVKASVTAQTAFVVPSAFTPNGDGHNDCFGI
                     QHWGDVTIEQLDVFNRQGMMVFTTKNPAECWDGRFKGQMQPTGAYVYAIRARTACGEI
                     TRTGVIMLIR"
     gene            2359..3099
                     /locus_tag="VKQ52_21275"
     CDS             2359..3099
                     /locus_tag="VKQ52_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89805.1"
                     /translation="MTIRKRIVLARLALITITLLSMGLFGFFKKQTDPKQTTAAMPGG
                     PYKDSTTNLIYNLLFCDNPDLYKANTKPPYSYPFDILFSEKSTVADLQKIIDDKNTDV
                     RVRVLAYNRQYAKGHKPNKKELLAVIVEVGLDGGLDVLASFANGTARYINQTGKILVW
                     EKTTDVKANALTNDLFAKSRQIVDQIGRWDKPRKPNPTSGNTRITFLVSDGLYFGEGP
                     TDDLFSDQLAGPALTSATQLMQYLTSRQ"
     gene            complement(3174..>3525)
                     /locus_tag="VKQ52_21280"
     CDS             complement(3174..>3525)
                     /locus_tag="VKQ52_21280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLZ89806.1"
                     /translation="DGSMHSLQACTAGQQTTRTAVSGMANPFENTDNIASILYPNPAH
                     DLVTIHITGEKQGTVVVHVYSAAGGLIQTRAAVKGASMLDVPVYLSGLAAGVYIVEIE
                     IGGAVWQRQKLLKL"
BASE COUNT          878 a          909 c          920 g          818 t
ORIGIN      
        1 attttggaac cagccttctc aatattccga cgcaggtgga tcttggcaac ttttcgcagg
       61 ccctgccgct gacaccagag gggatccagt taatacgagt gaacggcaaa tggatcgcca
      121 ttatcacagg cggcaacaca tacggagcca ataacgagtc aagggtgatc aaaatggatt
      181 tcggcaacag tcttgccaat actcccactg ctaccaattg gggcaatatc ggcgcgttaa
      241 attttcccga tgatatcttt atcgcaaacg atgggggtac ctatatcggc tatgcgatca
      301 atgtccttga caatacgttg acacggtgga atttcggcac cgatttttca ggagtaccga
      361 caggaaccaa ccttggcaac ccgggtggaa ccgtcgattg gcctgtgggc tcagggtatt
      421 tcaattacaa cggcaagtgg tatggtttta tagccaaccg gaattccaat tcgctgacgc
      481 gcctggattt cggtacttcc ctggcgaata cacccacggc tgtaaacatt ggtaacccgg
      541 gtgggctatt gaattatccc agggattttg ccatattcgc tacctgtggt gaaatttatg
      601 gatttgtgcc caatgaggga acgaatgcgc ttgtcaagct ggatttcgga tcggatccca
      661 ccaatcccag cccaacggca acttcgttgg gcaacatcgg taatctggac tatccgcatt
      721 cgataaccaa tcttttccgg gcggggaacg atgtttatgc cttcatatgc aacgcgtaca
      781 gcaatacgct ctcccgcatg cgatttgcgg ggtgtcagga tattcccggg tcgacggcag
      841 cagcacctgc gcccgttacc tatcacaccc ccgggaccta tacgatcagc ctgttggtgg
      901 atgttgggct tccgacgcag acttcctatt gccagaagat caccgtgctt gcttcgcccc
      961 tgggcacgat ccagggggac accgtctgct ttggcagcag ccctgccctg aactttgccg
     1021 gaacgggtac gggaccattc gacatcagct tcagtgatgg gaaccagacc tatacgcagg
     1081 gagggctccc ggtgcaatct tccattccct tgccaaatac actgacggct ccaggcagcg
     1141 ccgaatttgt tctgcaaaaa gtgtctgatg gaacagggtg ttcgacatcg ttgaatcaat
     1201 ccaccaatat tcttattcgc ccggctccgc agtcgggtat tacagggaca accatctgtg
     1261 gatccgattc tgcgctgatc tccctccagg ccggtgcagg cgtccccccg ttcgaagtgc
     1321 agatcagcaa tggaagtact tcatttgtgg tgaatggagt gaaacccgga gcagcttttc
     1381 ccgtggcgtt cccaggtatg aatgctgcgg cgaatttttc cctgatggaa gtgaaagata
     1441 gcgtgggctg cccggaggag acaggttttg ctccaggagt gacaaatgtg gttccgctac
     1501 ccgctccggc agttgtcttt ccatctttgg gcgcggtgtg ttatgataag gagccttttg
     1561 tccttggcac ggcaagcgag acgacgggcc ttgccggaac cggcgtctat agcggttcgg
     1621 gtattgctac cggcggcacc ttttcaccgg cggcggcagg cccgggagcg catgagataa
     1681 aatatactta tgtcgcagcc gacggttgta cggcagtcga cagcagcgtt attactgtca
     1741 acgcgttgcc taaagcaagc acggttcccc tgatcactgc ctgcgaaggt gttcccgtgc
     1801 gactgacggc cagcggaggc aatacctatc tgtggtcgcc ggctggcgat ctgagcgacc
     1861 cgacaagtca gacaccggtg gccactgtgg atacaacgac gatgttcata gtggaagtga
     1921 cggacagtaa tggatgttcc gcgaccgatt ccatattggt aaaggcctcg gtgaccgcac
     1981 aaacagcctt cgttgttccc agtgccttta cacccaacgg agatgggcat aacgactgtt
     2041 ttggcatcca acattggggt gatgtgacta tcgagcagtt ggatgtattc aatcggcaag
     2101 ggatgatggt ctttacaaca aaaaatcctg ccgagtgctg ggatggccgt tttaaggggc
     2161 aaatgcagcc gaccggggcg tatgtttatg cgataagagc gcggacagcg tgcggcgaga
     2221 tcacgcggac cggggtgatc atgcttatcc gatgaaccaa ttcatcgctg actcgaatga
     2281 aacgatagtc gatttcgggt ccttttctgg aagaaaacct atttcggtca aactatttgg
     2341 ggtttgacat ccataattat gacaatccga aaacgaatag tattagcaag actggccctg
     2401 ataacaataa ctcttttatc tatgggacta tttggctttt tcaaaaagca aactgatcca
     2461 aaacagacaa ccgcagctat gccaggcggc ccttataaag acagtacgac aaacctgatt
     2521 tacaacctgc ttttttgcga caatcctgat ctgtacaaag ccaataccaa accaccttat
     2581 tcctatccat tcgacatttt gttctcggaa aagagtacag tagcggattt gcagaagatt
     2641 atcgacgaca aaaacacaga cgtaagagta agggtccttg catacaaccg ccaatatgca
     2701 aagggacaca agccaaacaa gaaggaactt cttgcagtaa tagtcgaggt aggattagat
     2761 ggagggctcg atgttttagc ttcttttgca aatgggactg cccgttacat taatcagacg
     2821 ggaaagatcc tggtttggga aaagacaacc gacgtcaaag cgaatgcact gacaaatgat
     2881 ctgttcgcaa agagccggca aatcgtagat cagatcgggc ggtgggataa accaagaaaa
     2941 ccgaatccaa caagcggcaa cacaaggatc acctttcttg tctcggatgg cctttatttt
     3001 ggtgaaggac caacggacga tttatttagt gatcaattag ccggccccgc attaacgagc
     3061 gcaacacagc taatgcaata tctgacttct cgccaataga tacccgggcg acgtcgaagg
     3121 cgacaaaaag acgcggcccg gcgaagccgg gccgcgtcca tacaacaccc atcttataat
     3181 ttcaatagct tctgcctctg ccataccgca ccgccgatct cgatctccac aatataaacc
     3241 ccggcggcca ggcctgaaag ataaaccggc acatcgagca tcgatgcccc ttttacggcg
     3301 gcccgcgtct gtatcaatcc gcccgcggct gaatagacat gcaccaccac cgtaccctgt
     3361 ttttcgccgg tgatatggat ggtgacaagg tcatgcgcgg gattgggata gagaatggac
     3421 gcgatgttat cggtattttc gaatgggttg gccatgcccg acaccgccgt ccgcgtggtc
     3481 tgttgaccag cggtgcaggc ctgcaaagaa tgcatgctgc catcc
//
LOCUS       DATGFG010001124         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1145128, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001124 DATGFG010000000
VERSION     DATGFG010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145128"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VKQ52_21285"
     CDS             <1..559
                     /locus_tag="VKQ52_21285"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLZ89807.1"
                     /translation="LIESELFGHEKGSFTSAVKQRIGKFEQANGGTLFLDEIGDMSLT
                     AQAKVLRALQEGKITRVGGDKDISVDVRVIAATNKDLLKEVEEKSFRLDLYHRLSVII
                     IHVPSLNERRDDIPLLVEKFLVDICGDYGIARKAIEDEAIKALQQHNWTGNIRELRNV
                     VERLIILSGKTITEIDVESYVLPKK"
     gene            566..1216
                     /locus_tag="VKQ52_21290"
     CDS             566..1216
                     /locus_tag="VKQ52_21290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLZ89808.1"
                     /translation="MDCYCISGLGADHRIFSRLDLPGVDLRPLPWLTPVPYEPIAEYA
                     DRMRAGIDGGQPVLLGVSFGGMMAIEIARKLPGAVVIIVSSVRDHQQMPFWIKAGGRV
                     YPHWLIPRLRRPRWRPGFLEDYFLGVETDADRQLVREFRNTVDQAYLDWSVWTIARWR
                     NLWTPPSFYHIHGGRDRMFPLRKVQATQVIPDGGHLMIHNRAGEVSRALRDILRLC"
     gene            1305..>2253
                     /gene="lepB"
                     /locus_tag="VKQ52_21295"
     CDS             1305..>2253
                     /gene="lepB"
                     /locus_tag="VKQ52_21295"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLZ89809.1"
                     /translation="MSVHQLLVLVLIQVIILLLPSVGAARLFEKAGVSGLTAYIPFYN
                     TWVMLELAERPKHWVFWQLIPIVGWFISLGIFVEFVKIFGKFRLWEHALVVLLPLIYF
                     PLIGYDPRVRFIGAGPVRQHRKGTAREWVDAGVFAVIAATLIRTFVFEAYTIPTGSME
                     KTLLVNDFLFVSKFSYGPRIPNTPLSIPFVHNTLPYSNANSYVKWIKIPYVRWFASPV
                     KRGDVVVFNFPAGDTVINRDEYQSLHPYYSVCRALGGGNIDSGRQIVLGNPDQYPLVI
                     RPVDKEENYIKRCVAIAGDTLQIKDQVVYINGKAQPFPPE"
BASE COUNT          454 a          552 c          719 g          528 t
ORIGIN      
        1 actgatcgag tccgagcttt tcgggcatga gaaaggttcc tttacctctg ccgtcaagca
       61 gcggatcggg aaattcgaac aggcgaatgg cggcacgctc ttcctcgacg agattggcga
      121 tatgagtctt acggcgcagg ccaaggtgct gcgggcattg caggaaggca agatcacgcg
      181 ggttggcggg gataaagaca tcagtgtgga tgtgcgcgtg atcgcggcta ccaataagga
      241 cctgctgaag gaggtggagg agaaaagttt ccggcttgac ctctatcacc ggttgagcgt
      301 catcatcatt catgttcctt cgctcaatga gcggagggat gatatcccgc tgctggtgga
      361 gaaattcctg gtcgatatct gcggggatta tgggattgcc cggaaggcga tcgaggatga
      421 ggcgatcaag gcgttgcagc agcataactg gaccgggaat atccgggagt tgcgcaatgt
      481 ggtggagcgg ctgatcatcc tgtcggggaa gacgatcacg gagatcgatg tggagagtta
      541 tgtgttaccg aaaaaataag cgttattgga ctgctactgc ataagcgggc tgggggcgga
      601 tcaccggatc ttttcccggc tggacctgcc cggggtcgat ctgcggcccc tgccctggct
      661 gacgccggtg ccttatgagc cgatcgcgga atatgccgac cggatgaggg cgggcatcga
      721 tggcgggcag cccgtactgt taggggtctc ctttggcggg atgatggcga tcgagatcgc
      781 gcggaagctg cccggcgccg ttgtgatcat cgtatcgagt gtccgtgatc atcagcagat
      841 gccgttctgg ataaaggccg gtgggcgggt gtatccgcac tggttgatcc cgcgcctgcg
      901 ccggccgaga tggaggccgg gttttctcga ggattatttt ctgggggtag agaccgatgc
      961 cgaccggcag ttggtcaggg agttccggaa tacagtggat caggcctacc tggactggtc
     1021 cgtctggaca attgcgcgct ggcggaatct atggacccct ccctcttttt atcatatcca
     1081 tggcggcagg gaccggatgt tccctttgcg gaaggtacag gctacgcagg tgatacccga
     1141 tgggggtcat cttatgatcc acaaccgggc gggagaggtg agccgggcat tgagagatat
     1201 cctgcgattg tgctgaggcg ggtcagcagc cgcttgtgaa ggcgcatcgg gtaaaaacta
     1261 ccagttttga cctacatttg ccggtattca acaaaaatcc ccgaatgtcc gttcatcagc
     1321 tgctggttct tgtgcttatc caggtgatca tcctcttact tccctccgtc ggtgcagcca
     1381 gactctttga aaaagccggg gtatccggtc tgacagccta tattcctttt tacaatacct
     1441 gggtcatgct ggaactggcg gagcggccga agcactgggt cttctggcag ttgatcccca
     1501 ttgtcgggtg gtttatttcg ctgggcattt ttgtggagtt tgtgaagatc ttcgggaagt
     1561 tcaggctttg ggagcatgcg cttgtggtct tgttaccgct gatctacttc ccgctcatcg
     1621 gatatgaccc cagggtcagg tttatagggg ccggtccggt gagacagcat cgtaagggaa
     1681 cggcgcggga atgggtcgat gcgggggtgt ttgccgttat agccgcgacg ctgatccgga
     1741 ccttcgtgtt cgaggcctat acgatcccga cggggtcgat ggagaagacc ctgctggtga
     1801 acgattttct ttttgtcagc aaattcagct atgggccgcg gatcccgaat acccctttgt
     1861 cgatcccctt tgtgcacaat accctgcctt atagcaatgc caactcttac gtaaaatgga
     1921 tcaagatccc ttatgtgcga tggtttgcca gcccggtgaa gaggggagat gtcgtcgttt
     1981 tcaatttccc ggccggggat accgtgatca accgggacga ataccagtca ctgcatcctt
     2041 actacagtgt gtgccgggca ttgggtgggg ggaatatcga cagcggccgc cagatcgtgc
     2101 tgggtaaccc cgaccagtat cccttggtga tccggccggt ggacaaagag gagaactata
     2161 tcaagcgttg tgtggcaatc gccggcgaca cgctgcagat aaaggaccag gtggtgtata
     2221 tcaacggcaa ggcgcagccg ttcccgcccg aat
//
LOCUS       DATGFG010001125         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_636772, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001125 DATGFG010000000
VERSION     DATGFG010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636772"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VKQ52_21300"
     CDS             complement(<1..758)
                     /locus_tag="VKQ52_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HLZ89810.1"
                     /translation="MNCKEVQAAMIDYLDQALPAASAARIKEHLDSCSECRREVEDLQ
                     VLLTTMKAGVQEMPSPRLRENFNTMLQSELNMEAMANILQDPTSGKRPHATIVTIFSS
                     VAGRAAAAVILLAGGIGIGMAIKSGPETNNQAEINTLKTEVAKMEVKMKEQDMLSLID
                     DESASQRIKAVGYAEDMANPDQQVIDALFNSLNHDKNVNVRLAALYSLARFADRHTVR
                     DSLVSSLSTQNEPIIQVVLINLLAEKRESKAIAP"
     gene            complement(770..1309)
                     /locus_tag="VKQ52_21305"
     CDS             complement(770..1309)
                     /locus_tag="VKQ52_21305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLZ89811.1"
                     /translation="MPELSDEKIMLLVKEGHLSELTALFDRYQVSLYNFFLRLTFDRA
                     ASEDLTQNLFYRVIRYRHSYEPSHGSFRSWVYRMARNVHFDFCKQEQKAPGRLTDPEE
                     VDERLADKITGYTEDQYQRLEEALAQLAPEQREILVLSRYQGLKYEEISRIKDISVTA
                     IKVQVYRALKQLRTLYFNK"
     gene            complement(1384..1920)
                     /locus_tag="VKQ52_21310"
     CDS             complement(1384..1920)
                     /locus_tag="VKQ52_21310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ89812.1"
                     /translation="MQDSVTIRLMRPSDLDTLGFICRESYSQNFGHHWEPGGLEEYLE
                     KVFGAEILSKELQDNDIQYYVAYHEATPIAFMKLHFRSNLPGLEPEKGIELDKLYILP
                     AYKGLTIGRRMMSLALGLATAANKEICWLIVIDTNRDAITFYEKYGFRFHSAGRVPYP
                     RFREELKGMWRMGLEIKK"
     gene            2019..3272
                     /locus_tag="VKQ52_21315"
     CDS             2019..3272
                     /locus_tag="VKQ52_21315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ89813.1"
                     /translation="MDHLFRHESGKLVSVLTRIFGVENIDLAGDVVQDSVAEALSQWQ
                     YKGIPDNPSGWLFRVAKNKALNLLNREKYRRKYSADTRHSLLYGEAVESSLDGFFSDE
                     EILDDQLRMIFTCCHPALSPDSQIALALKTLCGFSVPEIARSFLTTEENIKKRLTRAK
                     QAIREDAVPFDVPAGEELHRRTGVVLETIYLLFNEGYSAYTGDDIVRYDLCAEAIRLA
                     GIIEQHPAIRDKSGVCALLSLMYFNASRFTARQDREGRLLTMAEQDRSLWDRSLIAKG
                     IDWLRRSTLPGNGSVYFLLAMISAHHCMAPDYASTDWKSILECYDSLVQVEHSPVVLL
                     NRAIALSKVEGPEKALRELEPLQDDTALQTYPLFYSTKAELHIQLNRFPAASDCLEKA
                     IAFSTLEKERRLLQAKLIACREKIS"
BASE COUNT          710 a          896 c          923 g          807 t
ORIGIN      
        1 atcggggcga tggcctttga ctctcttttc tccgccagca ggttgataag gacgacctgg
       61 atgatgggtt cgttctgggt gctcagggaa ctcaccagcg aatcccggac ggtatgcctg
      121 tcggcaaagc gggcaaggga atacaaagcg gccaggcgga cattgacatt tttatcgtgg
      181 ttaagggagt tgaagagggc gtcgatcacc tgctgatcgg gatttgccat atcttccgca
      241 tagcctacgg ccttgatccg ctggctggcc gactcgtcat cgatgaggga caacatatcc
      301 tgttccttca tctttacttc cattttcgcc acctcggttt tgagcgtatt gatctcggcc
      361 tggttgttgg tttccgggcc cgatttgatg gccattccga tcccgatgcc cccggccagc
      421 aggatgaccg cggcggcagc cctgccggcc accgaagaga atattgtcac gatagttgcg
      481 tgtggtcgtt tgccggaggt gggatcctgc aggatattgg ccattgcctc catattgagt
      541 tccgactgca gcatggtgtt gaagttctcc cgcaggcggg gcgaaggcat ctcctgaacg
      601 ccggccttca tggtcgtcag caacacctgc aggtcctcca cttcccggcg gcattcgcta
      661 caggagtcca ggtgttcctt tatccgcgcg gcggatgcag caggtagcgc ctggtccagg
      721 taatcgatca tcgccgcttg tacttcctta caattcatgg tcttgctttc tatttgttaa
      781 aataaagggt cctgagctgt ttgagggccc ggtacacctg taccttaatc gccgttaccg
      841 agatatcctt gatccgggag atctcctcgt atttcaaacc ctggtagcgg cttagcacca
      901 ggatctcccg ctgttcgggc gccagttgcg ccagcgcttc ttccagccgc tgatactgat
      961 cttctgtata ccctgttatc ttgtccgcca ggcgttcgtc tacctcttcc gggtccgtca
     1021 ggcggcccgg ggccttttgc tcctgtttac agaagtcaaa atggacattg cgtgccatcc
     1081 ggtagaccca gctccggaaa ctgccgtgcg agggttcata actatgacgg taccggatga
     1141 cgcgatagaa aaggttttgc gtcaggtcct cgctggcggc ccggtcaaag gtcagccgga
     1201 ggaaaaaatt atacaggcta acctggtacc ggtcgaataa tgccgtcagt tcggacagat
     1261 gcccctcctt taccagcagc attatttttt catcagatag ttcaggcacc cgggataaca
     1321 tttaaggttt ctatagagat tactgcggaa gggcaaaagg gttacaggca aacgggaact
     1381 tttttatttc tttatttcca gccccatgcg ccacatccct ttcaactctt cccggaatct
     1441 gggatagggc accctgccgg cgctatggaa ccggaaccca tacttctcgt agaaggtgat
     1501 ggcgtcccgg ttggtgtcga tgacgatcag ccagcatatt tccttgtttg ccgccgttgc
     1561 gaggcccagg gccaggctca tcatccggcg cccgatggtt agccccttgt aggcggggag
     1621 gatatagagt ttgtccagtt cgatgccctt ttccggctcg aggccgggca ggttggaccg
     1681 gaaatgcagt ttcataaagg cgatgggagt cgcctcgtga tatgctacat agtattgaat
     1741 gtcattgtct tgcaattctt ttgacaagat ttccgctcca aacactttct ccaggtattc
     1801 ttccagcccg ccgggttccc aatgatgccc gaagttctgg ctgtaggatt cccggcagat
     1861 aaagccgagg gtgtcgaggt cggacggccg catcaggcgg atggtgacag agtcttgcat
     1921 gacagattga agggattggt tgacgaagat aggcacaggt ttcggtatat tggggcataa
     1981 aaagcaagct catagaacag ccagccatca accagcttgt ggaccacctt ttccggcatg
     2041 agtccgggaa actggtctcc gttctgacgc ggatcttcgg cgtggagaat atcgatctgg
     2101 ccggggatgt ggtgcaggat tccgttgccg aggccctcag ccaatggcaa tacaagggca
     2161 tcccggacaa tccttccggc tggcttttcc gggtggcgaa gaacaaggcg ctgaacctcc
     2221 tgaaccggga gaagtacagg cggaaatatt cagccgacac ccggcattct cttttatacg
     2281 gggaggcagt tgaatcctcc ctggatggct tcttttccga tgaggagata ctggacgatc
     2341 agttgcgcat gatctttacg tgttgccatc cggctctttc ccccgattcc cagatcgcgc
     2401 tggcgctaaa aacgctctgc gggttcagcg tccccgagat cgcaaggtct tttctcacca
     2461 cggaagagaa tataaaaaaa cgcctgacaa gagcgaaaca ggctatccgg gaggacgccg
     2521 ttccttttga tgttccggca ggagaggaac tgcaccggcg caccggagtc gttctggaaa
     2581 cgatctatct tctgttcaac gagggctata gcgcctatac cggtgacgat atcgttcggt
     2641 atgacctttg cgcggaggcg atccggctgg cagggatcat tgaacaacat cctgccatcc
     2701 gggataaatc gggcgtttgc gccctgttgt cgctgatgta tttcaatgcc tcgaggttta
     2761 ccgcgagaca ggatcgggaa ggccggttgc tgacgatggc ggagcaggac cgttctctct
     2821 gggatcgttc gcttatcgca aaaggcatcg actggttgcg tcgatcgacc ctcccgggca
     2881 atggctctgt atacttttta ctcgcaatga tctccgcgca tcattgcatg gccccggact
     2941 atgcgtccac cgattggaag agcattctcg aatgctatga tagcctggtg caggtggagc
     3001 actctcccgt ggtgctactg aacagggcca tcgcactgtc gaaggtggaa ggccccgaaa
     3061 aggcattgcg ggaactggaa cccttacagg atgataccgc ccttcagacc tacccgctct
     3121 tttattctac aaaggccgaa ctccatatac agttgaaccg gttcccggcc gcctcggact
     3181 gcctggaaaa agccatcgcc ttttcgaccc tggaaaaaga acggcgcctg ttacaggcga
     3241 aactcatcgc ctgcagggaa aaaataagtt gagggccatg tcccctgtgg gaaactccat
     3301 ttgtctccaa tcaaacaata aaaacagaaa aaatgg
//
LOCUS       DATGFG010001126         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_637109, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001126 DATGFG010000000
VERSION     DATGFG010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637109"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1110
                     /locus_tag="VKQ52_21320"
     CDS             85..1110
                     /locus_tag="VKQ52_21320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLZ89814.1"
                     /translation="MRKTLLFFLSISALQLYAQTSADSTPALRQLPGLYCIVLSVKDT
                     IRYDQHFNGTKTEDVFDDQSLTKSLGSLLVGIAIDKGLIASLDEKLVHWFPQLEKDTD
                     RRKQDITLRQVMNQASGLRHEDLEKMNGIADYLALSDPAGFVLDAPLLSTPGAVFHYN
                     NAATHLLSLIITKSSGMDVLSFARKNLLAPLGITAIEWTKMKDGYYDLAGLENIRLRT
                     ADWVKIGSLLLHEGRYGRQQVVPAKWIAQVLHPDVLYPTPWGFRPSQYGLCWYHFTYQ
                     GQEMIYGMGWGGQFLVVVPGLQMVLAINQSREDRNAIPHAGRFIESIFPRFLDAVSRH
                     QPLENSF"
     gene            complement(1124..2767)
                     /gene="pgm"
                     /locus_tag="VKQ52_21325"
     CDS             complement(1124..2767)
                     /gene="pgm"
                     /locus_tag="VKQ52_21325"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HLZ89815.1"
                     /translation="MPINPMAGKPADPQQLVDLTRLTTAYYTETPDPGVPAQRVAFGT
                     SGHRGSSFDAAFNEAHILAITQAICLYRQEQRIDGPLFLAVDTHALSWPAQISALEVL
                     AANGVEVMIADGTDYTPTPVLSHAILGYNQQRTTGLADGIVITPSHNPPRDGGFKYNP
                     PHGGPAEPAITDWVQKKANEFLGNGLQGVKRMIYSKALHAPTTHKYDYRKPYIDDLAN
                     SIDMDRIRDSKLHFGVDPLGGAGVHYWQPIADHYGLDLTVVSTTVDATFRFMTVDWDG
                     QIRMDPSSPYAMAKLVGLKDKFDIAFACDTDHDRHGIVTRSMGLMQPNHYLSAAIYYL
                     FQHRPKWAATTRIGKTLVSSQMIDRVATKLKRELYETPVGFKWFVDGLLAGTLGFVGE
                     ESAGASFLRSSGKVWTTDKDGMIAGLLAAEITAVTGKDPGQLYQELTRELGSPVYDRI
                     EAKATPEEKKKLSKLSPSMIKQKEMAGEKITGILTTAPGNDAPIGGLKVTTENGWFAA
                     RPSGTEDIYKIYAESFRGADHLKQIQEQAQAIVNEALKA"
     gene            2911..>3540
                     /locus_tag="VKQ52_21330"
     CDS             2911..>3540
                     /locus_tag="VKQ52_21330"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HLZ89816.1"
                     /translation="MNQQTLKTNQLFGFLPSEVEGLDTLLGLALDMRWSWNHAADELW
                     RQLDPAIWDLTHNPWVILTTLSREELQARLTDPSFREKVNTLEAEKEQSAASPAWFQK
                     KYPDSLLQNVAYFSMEYMLSEALPIYVGGLGNVAGDQLKSAADLGVPVTGVGLLYQQG
                     YFRQAIDRNGNQQAMFPYNDPMQLPITPVRLPNGEWLRIKVTLSGLPVWL"
BASE COUNT          744 a         1039 c          941 g          816 t
ORIGIN      
        1 actcaaaccg tgctcagaat tgtttttgaa gtgctctgcg gtgcagcggg aaattgtata
       61 attttagccc cttaagctat agctatgcga aaaacacttc tgttcttcct gtcgatcagt
      121 gctttacaat tgtatgctca aacctccgcc gatagcacac ccgcccttcg ccaactgccc
      181 ggtctgtatt gcatcgtcct ctcggtaaag gataccatcc ggtacgacca gcatttcaat
      241 gggacaaaga cagaagatgt cttcgacgat cagtccctga ccaagagcct cggaagcctc
      301 ctcgtcggta tcgccatcga taaggggctt attgcttccc ttgacgaaaa actcgtacac
      361 tggttcccgc agctggaaaa ggataccgac aggcgaaaac aggatatcac cctccggcag
      421 gtgatgaacc aggcttccgg tctgcgccac gaggatctgg agaagatgaa tggcatcgcc
      481 gactacctgg cgctgtccga tcccgcgggc tttgttctcg atgccccgct gctgtctacg
      541 cccggcgcgg tctttcacta taataatgcg gctacacacc tcctgtccct gatcattacg
      601 aagagcagcg gaatggatgt gctcagcttc gccagaaaga acctgctcgc ccctctcggc
      661 ataaccgcga tcgaatggac aaagatgaaa gacgggtact atgacctggc cggtctcgag
      721 aatatccggc tccgcacggc cgattgggta aagatcggct ccctgttatt gcacgagggg
      781 cgttatggcc ggcaacaggt ggtgcccgcg aagtggatcg cccaggtcct ccatcccgac
      841 gtcctctatc ctaccccctg gggtttccgg ccctcccaat acggcctctg ctggtatcat
      901 ttcacttacc agggacagga gatgatctat gggatgggct ggggtggaca gttcctcgtc
      961 gtcgtccccg gcctgcaaat ggtactggcc atcaaccaaa gccgggaaga ccggaatgcg
     1021 atcccacatg ccggaagatt catcgagtcc atcttccccc gcttcctcga cgccgtatcc
     1081 aggcaccaac ctctcgaaaa ttctttctaa tatggcaaac ctcctaggct ttcaacgcct
     1141 cgtttacgat cgcctgggcc tgttcctgta tttgtttcaa atgatctgct ccgcggaagc
     1201 tctccgcata gatcttgtag atatcctccg ttcccgatgg ccgtgcggca aaccacccgt
     1261 tctcggtggt cacctttagc ccgccgatcg gggcatcatt accgggagcg gtagtaagga
     1321 tgccggtgat cttttcaccg gccatttcct tttgtttgat catcgaaggg gagagtttgg
     1381 acagcttctt cttctcttcc ggtgtcgcct ttgcttcgat gcggtcatat acggggctgc
     1441 ccagttcccg ggtcagttcc tggtatagct gtcccggatc cttccccgtg acggccgtga
     1501 tctcggcagc aagcagcccg gctatcattc cgtctttatc cgtcgtccac accttcccgc
     1561 tgctgcggag aaaggaggcg cccgcgcttt cctcacccac aaagcccagg gttccggcaa
     1621 gcagtccatc cacgaaccac ttaaatccta cgggtgtttc atatagctcc cgtttcagtt
     1681 tcgtggcgac ccggtcgatc atctgactgc tgacaagcgt cttcccgatc ctcgtcgttg
     1741 ctgcccactt cggccgatgc tgaaacaaat agtagatggc cgcggaaagg taatgattgg
     1801 gttgcatcag ccccatcgat ctggtgacga tgccatgccg gtcatgatcg gtgtcgcagg
     1861 cgaaagcgat atcgaattta tccttcaatc caaccagctt tgccatcgca taaggggagg
     1921 acgggtccat ccggatctgc ccgtcccaat ccaccgtcat aaagcgaaaa gtggcgtcca
     1981 ccgtggtcga tacgacggtg aggtcaagcc catagtgatc cgctatcggc tgccagtaat
     2041 gaacgccggc gcccccgagc gggtcgaccc caaaatggag tttcgagtcc ctgatccggt
     2101 ccatatcgat actattcgcc aggtcgtcga tatagggttt ccggtaatca tacttgtgcg
     2161 tggtgggagc gtggagggct ttcgaataga tcattcgctt tactccctgc agcccgttcc
     2221 cgagaaattc attcgccttc ttctgcaccc agtcggtgat agccggttcc gcggggccgc
     2281 catgcggtgg attatatttg aagcccccgt cccggggagg gttatgggaa ggcgtgatca
     2341 cgataccatc ggcaagcccg gtggtccgct gctggttata gccaaggatc gcatgcgaga
     2401 gtaccggcgt cggcgtgtaa tcggtgccat ccgcgatcat cacttccacc ccgttggccg
     2461 ccaatacttc cagtgcggag atctgcgccg gccaggagag ggcgtgcgta tccaccgcaa
     2521 gaaataaagg accatcgatc cgttgctcct gccggtacag gcagatcgcc tgggtaatgg
     2581 caaggatatg cgcttcgtta aaggcggcgt cgaaggaact gccccgatgc cccgatgtcc
     2641 caaaagctac ccgttgcgcg ggaacaccgg gatccggcgt ctccgtgtaa taggcggtgg
     2701 tcaatcgcgt aaggtcgaca agttgctgcg gatcggcagg ctttcccgcc attggattga
     2761 ttggcatagt tgtatttttt acggcttgtt cgtaccggga tacaaaagta atacccgttt
     2821 gatttgcaca cttattattt cccggttaaa agcaaatccg ggatgaacat tcctgctaaa
     2881 tggctgtata ttcaggcaaa atcgatccga atgaatcagc aaacgctcaa aaccaatcaa
     2941 cttttcggtt ttttgccttc ggaagtggaa ggcctggata ccctccttgg attggccctt
     3001 gatatgcggt ggtcatggaa tcatgcggcc gacgagctct ggcggcaact cgaccccgcc
     3061 atatgggatc tcacgcataa cccctgggtc atcctgacga cgttgtcccg ggaagagcta
     3121 caggcccggc tgaccgaccc ttccttccgg gagaaggtca atactctcga agccgagaaa
     3181 gagcagtccg cggcgagccc cgcctggttc caaaaaaaat accccgactc gctgctacaa
     3241 aacgtggcct attttagcat ggaatatatg ctgagcgaag ccctgcccat ctatgtcggc
     3301 ggcctgggca acgtcgcggg cgatcagttg aagtccgcgg ccgatcttgg cgtgccggta
     3361 acaggggtcg ggctgctcta ccagcagggc tatttccgcc aggcgatcga tcgcaatggc
     3421 aaccaacagg cgatgtttcc ttataacgat cccatgcagt taccgatcac ccccgtacgg
     3481 ctgcccaacg gagaatggct gcggataaag gtcaccctgt ccggcctccc cgtgtggttg
//
LOCUS       DATGFG010001127         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1093256, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001127 DATGFG010000000
VERSION     DATGFG010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093256"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VKQ52_21335"
     CDS             <1..559
                     /locus_tag="VKQ52_21335"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HLZ89817.1"
                     /translation="IGVATSKEPAANFTVRGYIQGLESMIDPCVFVDDDGQAYLYYGG
                     GGKCRGGRLKDNMMEIDGAMQPMTGLEDFHEASWVHKRKGVYYLSYSDNHDSAGQHNR
                     MRYATSNSPLGPWKYQGIYMDPTDSYTNHGSIVEYKGQWYAFYHNSALSHFDWLRSIC
                     VDKLFYNADGTIQKVVQTKTGPGTR"
     gene            complement(774..1064)
                     /locus_tag="VKQ52_21340"
     CDS             complement(774..1064)
                     /locus_tag="VKQ52_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89818.1"
                     /translation="MKQIKGLILLPLLAAVLFFGSFVARPEKNTKANILNYAWYTTDG
                     QFVAWSTLANAEFVTGADTNPVNGTLVSLGYTGGDPGQPPSGTLVYRLYTHP"
     gene            complement(1196..2731)
                     /locus_tag="VKQ52_21345"
     CDS             complement(1196..2731)
                     /locus_tag="VKQ52_21345"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLZ89819.1"
                     /translation="MHIDTRTLPDNTVIEGDICIVGTGPAGLSIAMEWNNTPYRVILL
                     EGGGFDYDDQVQDLYAGRTTGQPYFPLKSSHLHYFGGTSNHWGGFCSTFDELDFEQRS
                     WVPHSGWPIKKADLDPFYARAHPILDLGPCEYDETYWQKKDPALKSLLPDNPAVRNKV
                     WQFSTPTRFGPKYGPAVKQSKNIHLYTYANVTDIEANDNVSVIESLTLRNFAGKRHTV
                     KAKRFVLACGAIQNARILLACNRQAPKGLGNDNDLVGRFFMDHLELKSAYLHLPTADS
                     LKLYMLEWGKTKMRCELAISREIQARHAILNGTASLKSINEANIDTPAMKSWTSDDPR
                     KNGHVGFVRDHLARSISLADRTFGVSKYMLYTRIEQAPNPDSRITLDGVSDPLGMPRA
                     ILHWELTPLERRSIRTIYELIGRQFGAAGRGRTQLMDYLEDPDDASWPDFASGGWHHI
                     GTTRMSDDPHTGVVDANCKVHGIGNLYIAGSSCFVTAGAPNPTLTIVALSLRLSDHLK
                     KLT"
     gene            complement(2740..3141)
                     /locus_tag="VKQ52_21350"
     CDS             complement(2740..3141)
                     /locus_tag="VKQ52_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89820.1"
                     /translation="MKRRTFIFLAGATALTIPFGVCRHRSSSLIQTLGQPDFLGRICD
                     ETTIADIGAAYCARTPDESQEEKLVSLLLANRDGNHDRSSSAIDSSRLVRQLNLAIQD
                     DYTAGRVVTVKGWVLSLTEARQCALYTLSHQ"
     gene            complement(3282..3731)
                     /locus_tag="VKQ52_21355"
     CDS             complement(3282..3731)
                     /locus_tag="VKQ52_21355"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HLZ89821.1"
                     /translation="MSETWNKKERERNKQQKRKEKQEKKQEKKQAGKSTNSLESMLAY
                     VDENGNLSSQPMDPRKKLVVNVEDIDISIPKQRDALPEDQVQVGIVTLFKTDKGYGFI
                     KNPQTQQSYFFHIKSLIDPVKENNKVSFETEKGPKGPSAINIRQFKA"
     gene            complement(3987..>4214)
                     /locus_tag="VKQ52_21360"
     CDS             complement(3987..>4214)
                     /locus_tag="VKQ52_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89822.1"
                     /translation="QPIEAKIFFKTRSTVEGAFIKSPDYLELKRKNFWRIVSRVNLAN
                     YRASGDINLSRIFNGSEFVKLSPNDSARSGS"
BASE COUNT          886 a          991 c         1282 g         1055 t
ORIGIN      
        1 gatcggtgtc gccacgagta aggagccggc agccaatttt accgtacggg gttatatcca
       61 gggactggaa tcgatgatcg acccctgtgt ctttgtggat gatgacggcc aggcctatct
      121 gtattatggg ggcggtggca aatgccgcgg cggcaggcta aaggacaaca tgatggagat
      181 agacggcgca atgcagccga tgacgggact ggaggatttc cacgaggcaa gctgggtaca
      241 caaacggaag ggtgtttatt atctttccta ttccgacaac catgattcgg ccggtcagca
      301 taaccggatg cgttatgcca ccagtaacag cccgctgggg ccatggaaat accagggcat
      361 ctatatggat ccgacggaca gttacaccaa tcatgggtcg attgtggagt acaaggggca
      421 gtggtatgcg ttttatcata acagcgcgct gtcgcatttt gactggcttc ggtcaatttg
      481 cgtggataag ctgttttaca atgccgatgg gacgatccaa aaggtcgtgc agacaaagac
      541 agggccgggc accaggtgat tcccggtaag ccaaattcca ggcctggatg gtgcgaaata
      601 ccgactggaa atgcaggagg cgatggcccg gtacaatgcc gggaaggcgg aagaaaagaa
      661 gatgaagaag ccctggtgga agatctgggt atagaccaat accaacataa accggccaga
      721 cgatagtctg gccggcattt tttgtagtat aatccgcaag gtggagccgg ttgctaggga
      781 tgcgtataaa gtctgtacac cagcgtgccg gaaggcggct gacccggatc accaccggta
      841 taccctaacg agacgagcgt cccgttgaca gggttggtat ctgctcccgt cacgaattcc
      901 gcgttcgcca gcgtgctcca ggcgacgaat tgtccatcag tcgtatacca ggcataattc
      961 aggatgttcg ctttggtgtt cttttccggc ctggcaacga atgagccgaa aaataatacc
     1021 gcagccagca agggtagcag gatgaggcct ttgatctgtt tcatttgaca tgtttaatga
     1081 ttacgaaaaa ggtgaacacc ggcaagatgc tttttttcca tgcaatgtag acgcagtctg
     1141 gggcgggaag gaagtccggt gtcagaaggg aaggaaggcc ggtgttatcg gggcatcagg
     1201 tcagcttttt caagtgatcc gagagccgaa ggctaagggc tacgatcgta agggtggggt
     1261 tgggggcccc ggcggtgacg aagcaactcg acccggcaat gtacagattg ccaatgccgt
     1321 gcactttgca attggcgtcg acgacgccgg tatggggatc gtcgctcatg cgggtggtac
     1381 cgatatggtg ccagccgccg ctggcaaaat cgggccagct ggcgtcgtcg gggtcctcga
     1441 gatagtccat cagttgggtg cggcccctgc cggccgcgcc gaactggcgg ccgatgagtt
     1501 cgtagatggt gcggatgctc cgtctttcca atggggtgag ctcccaatgg aggatggccc
     1561 gtggcatgcc gagggggtcg cttaccccgt cgagggtgat gcgcgagtcg gggttgggcg
     1621 cctgttcgat ccgggtatac aacatatact tgctgacgcc gaaggttctg tcggcgagcg
     1681 agatggaacg ggctaaatga tcacgcacaa agccgacatg gccatttttt cgcgggtcgt
     1741 cgctggtcca ggatttcatg gccggtgtat ctatgttcgc ctcgttgatg gactttaggg
     1801 aggcagtgcc gttgaggatg gcatgcctgg cctggatctc gcggctgatg gccagctcgc
     1861 agcgcatttt tgttttgccc cattccagca tatacagttt gagcgaatct gccgtgggga
     1921 ggtggagata ggcggacttc agctccaggt gatccataaa gaaacgtccc acgaggtcgt
     1981 tgtcgttgcc gagacctttt ggcgcctggc ggttgcaggc gagcaggatg cgggcgttct
     2041 ggatggcgcc gcaggcaagg acaaatcgtt ttgctttcac ggtatgtcgc ttgccggcga
     2101 aattccttaa cgtgagactt tcgatgaccg agacgttgtc attggcttcg atatccgtaa
     2161 catttgcgta ggtatagaga tggatattct tcgactgttt aacggccgga ccgtatttcg
     2221 gaccgaagcg ggtcggcgta ctgaattgcc agaccttgtt ccgcacggca ggattatcgg
     2281 ggagcaatga ttttagcgcg gggtcctttt tttgccagta tgtttcgtca tattcacagg
     2341 ggcccaggtc gaggatggga tgggctcttg cataaaatgg gtcgaggtcg gcttttttta
     2401 tgggccagcc gctatgcgga acccaggacc gctgctcaaa atccagttcg tcgaaggtgg
     2461 agcagaagcc cccccagtgg ttgctggtcc cgccgaagta atggaggtgg gaggatttga
     2521 gggggaagta gggttggccc gtggtgcggc cggcatagag gtcctgcacc tgatcgtcat
     2581 aatcgaaccc ccctccttcg aggaggatca ccctgtacgg cgtgttattc cattccatcg
     2641 cgatgctaag gccggcgggg cctgtgccga cgatgcagat atccccttca atcactgtgt
     2701 tatcgggtaa ggtacgggtg tctatgtgca tgggtcgttc tattgatggc tgagtgtgta
     2761 aagggcacat tgacgggctt cggtcaggga aaggacccag cccttgacgg tgacgaccct
     2821 gccagccgtg tagtcatcct ggatggccag gttcagctgc cggacgaggc gggagctgtc
     2881 aatcgcggag ctggatcgat cgtgattgcc gtcgcgattt gcgaggagga gagagaccag
     2941 tttttcttcc tgagattcat ccggcgtccg ggcgcagtag gcggcgccga tgtcggcgat
     3001 ggtcgtttca tcgcagatac gtcccaggaa atcgggttgc ccgagggtct ggatgagtga
     3061 actgctgcga tgacgacaaa ccccgaaagg gatggtcaaa gcagtggcgc cggccaggaa
     3121 tataaaagtc ctccttttca tatcgacggt aacagggtta gaacgaagag attgcgtttt
     3181 tattttccgc cgatgagggg aattgccagg agctccccat cggggaactc ctgcgggggt
     3241 gcaattatgt aaacgatggg gaaaatcgtg cgcggttact gtcaggcctt gaactgccgg
     3301 atgttgatgg cgcttgggcc cttggggcct ttttcggttt cgaagctgac cttgttgttc
     3361 tccttgaccg gatcgatcag ggatttgatg tggaagaaat agctctgttg ggtttggggg
     3421 tttttgatga aaccatagcc cttgtcggtt ttgaagagcg tgacgatgcc cacctgcacc
     3481 tggtcttcgg gtaaggcgtc tctttgcttg ggaatgctga tgtcgatgtc ctcgacattg
     3541 accaccagtt tctttctcgg atccatcggc tgggacgaga gattcccatt ttcgtcaaca
     3601 taagccagca tgctttccag ggaattcgtg cttttaccag cttgcttttt ttcctgcttt
     3661 ttttcctgtt tttctttccg tttctgctgc ttgtttcttt cgcgttcttt cttgttccat
     3721 gtttctgaca tttttttctc ctgtttttgt ttaagaattc ggaaaaccta cctcatcaat
     3781 gcaaaaagcc gacccgattt tgctggcggg atctccgttt ggcaacattc tggctcccaa
     3841 ttaattgtca atggtacggc tattttgtgg atttcccact atgacaaatc tggtataatt
     3901 ggccttttta tgagacaaga aggcagtatt gcccttatat actggcttaa tgacgggata
     3961 aagggcgaga tgcctgccgg cgcttgctaa ctgccggagc gagcgctgtc atttggggac
     4021 agctttacaa attccgaacc gttgaagatc ctggagaggt tgatgtcgcc tgatgcccgg
     4081 taattggcaa gatttacccg cgagacgatg cgccagaaat tcttcctttt caactcgagg
     4141 tagtccggag actttatgaa ggctccctct accgtactcc ttgtcttaaa aaagattttt
     4201 gcttctattg gctg
//
LOCUS       DATGFG010001128         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_70314, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001128 DATGFG010000000
VERSION     DATGFG010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70314"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VKQ52_21365"
     CDS             <1..290
                     /locus_tag="VKQ52_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89823.1"
                     /translation="QGLPPKQIQPPKRPQALHAASHPSTAGKNKKNPPPRQTVLYQRF
                     YRPGLARLREAATLRNLDQKNIFRQFFAAIQIPSAKTILTYLKTITPHLWT"
     gene            281..952
                     /gene="ung"
                     /locus_tag="VKQ52_21370"
     CDS             281..952
                     /gene="ung"
                     /locus_tag="VKQ52_21370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLZ89824.1"
                     /translation="MDVKIEESWKEVLKQEFTKPYFLQIVHHLKTERIAGKTIYPPGG
                     LIFNAFNTTPFEKVKIVLLGQDPYHGPGQAHGLSFSVPKGIPPPPSLVNIFKELHQDI
                     GMPIPNHGNLSHWAEQGVLLLNASLTVRANEPMSHAKIGWAEFTDSVIAKVSAQKEHT
                     VFLLWGKFAQEKQSLVDETKHLVLKAAHPSPFSVDKGFYGCKHFSKANAWLMKNGIDP
                     IDWML"
     gene            1146..2000
                     /locus_tag="VKQ52_21375"
     CDS             1146..2000
                     /locus_tag="VKQ52_21375"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLZ89825.1"
                     /translation="MNQPEGNTSTPTPGASAPTPGTPNSGANPAATRQSSPLWTILGP
                     LWAVWVILLFTITMLLFLPFFLLFSYFRPDPQKTTAFASMARIWMAVFLPLAGSPLRV
                     RGREKFAPGQTYIVVCNHNALLDVPISYPGIPGGNKTIAKIEMARIPLFGMIYRTGSV
                     LVDRHNEASRRESFSKMKEALDMGLHMCIYPEGTRNKTADPLKSFHDGAFRLALNTGK
                     PILPAIIFNTRKVMPPGKSLYFHPHRLYMRFLDPITPQPGDTVETLKQQVFKLMWENY
                     TAGPQTRP"
     gene            complement(1985..2122)
                     /locus_tag="VKQ52_21380"
     CDS             complement(1985..2122)
                     /locus_tag="VKQ52_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89826.1"
                     /translation="MISRSAAARFAAGELGAGEFAVAEFAVATVRFAAGEFVAGGQGR
                     V"
     gene            complement(2112..>3564)
                     /locus_tag="VKQ52_21385"
     CDS             complement(2112..>3564)
                     /locus_tag="VKQ52_21385"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HLZ89827.1"
                     /translation="KIVNVSLPNIAFTAPTIYPFARSTFVGTPKWYEKFGIGLSTTIA
                     GGASFYDSLFSLKRITDTFQWGAQNSIPLSLALPLKGPVQVSPGISLQNRLYSRKLFR
                     KYDPISNKVDTVGIQKGVFYANDMSFSLSLATAIFGTFQNFGRNSTLMGIRHVIRPTI
                     SISYKPDLSGPYYYNLTTPYDTTAGASKLSHVQRVSYFDGSTYGPFGEGRFGGISFGL
                     DNHIEIKTRNKSDTSTSASKKITLIDGFGFNGSYNYLADSFKLSPITFYFRSTLFNSI
                     NITGGATLDPYQTDSMGFDRNKYAWDAKKFSLGRITTGNVAISTSFKSKPKDQKLEQQ
                     KQQLMNSQNAMPMTMEEQQAQLNYMRNNSAQYADFNIPWSLNLSLAFNFTNAEKPDYS
                     GFQTTITSSLNWSGDFNLTDKWKIGLNGYYDLRYTKLQSLTMNISRDLHCWQMSINVT
                     PVGYSHYFNITINPKAGILQDLRINRTKYFYDQ"
BASE COUNT          827 a         1289 c          762 g          686 t
ORIGIN      
        1 accaggggct acccccaaaa caaatccagc cacccaagcg gccacaggcc ctccacgcgg
       61 ccagccaccc ctcaacggcc ggcaaaaata aaaaaaatcc ccctcctcgc caaaccgttt
      121 tgtatcaacg gttttacaga ccagggctcg cccggctccg cgaagcggcg acattgcgca
      181 acttggacca aaaaaatatt tttcgccaat tttttgccgc aattcaaata ccttcggcca
      241 aaaccattct cacctatcta aaaaccatta cccctcatct atggacgtaa agatcgaaga
      301 aagctggaaa gaagtgctga aacaggaatt cacaaaaccc tattttctcc agatcgtcca
      361 ccacctcaaa acggaacgca tcgccggcaa aaccatctac ccccccggcg gcctcatctt
      421 caacgccttc aacaccaccc ccttcgaaaa ggtaaagatc gtcctcctcg gccaggaccc
      481 ctaccacggc cccggccagg cccacggcct cagcttctcc gtccccaaag gcatcccccc
      541 gcccccatcc ctcgtcaaca tattcaagga gctccaccag gacatcggca tgcccatccc
      601 caaccacggc aacctcagcc actgggccga acagggcgtc ctcctcctca acgcctccct
      661 caccgtccgc gccaacgaac cgatgagcca cgccaagatc ggctgggccg aattcaccga
      721 ctccgtcatc gccaaagtct cggcgcaaaa agaacacacc gtcttcctcc tctgggggaa
      781 attcgcgcag gaaaaacaat ccctcgtcga cgaaacaaaa cacctcgtgc taaaagcggc
      841 ccacccctcc cccttttccg tcgacaaagg cttctatggc tgcaaacact tctcaaaggc
      901 aaacgcctgg ctcatgaaga acggcatcga ccccatcgac tggatgctct gacggcccgt
      961 tgaccgcaac atccctacac cggacatcgg acgttccccg gcaccgcaaa accgatcgcc
     1021 gcgcgccgcc gggtcgcgct ctccgttata gtcctcgcgg cccccgctat cgccggccgc
     1081 tgcgggctgc cacttcgatc gctaacgcaa tccaccagac accccggaaa aaactaaatt
     1141 gcagcatgaa ccaacccgaa ggcaacacct cgacaccaac acccggcgcc agcgcaccca
     1201 cccccggcac ccccaactcc ggtgcgaacc ccgctgccac ccgccaaagc agccccctct
     1261 ggaccatact cggccccctc tgggccgtat gggtcatcct cctgttcacc atcaccatgc
     1321 tgctcttcct ccccttcttc ctcctgttca gctacttccg gcccgacccg caaaaaacca
     1381 ccgccttcgc cagcatggcc cgcatatgga tggccgtctt cctccccctc gccggatccc
     1441 ccctccgcgt ccgcggccgc gaaaaattcg cccccggcca gacctatatc gtcgtctgca
     1501 accataacgc cctcctcgac gtccccatct cctaccccgg catccccggc ggcaacaaaa
     1561 ccatcgccaa gatcgaaatg gcccgcatcc ccctcttcgg catgatctat cgcacgggat
     1621 cagtccttgt cgatcgccat aacgaagcct cccgccgcga gtccttctcc aaaatgaaag
     1681 aagccctcga catggggctt catatgtgta tctatcccga aggcacccgc aacaaaaccg
     1741 cggatcccct caaatccttc cacgacggcg ccttccgcct cgccctcaac accggcaaac
     1801 ccatcctccc cgccatcatc ttcaacaccc gcaaggtcat gccccccggc aaatccctct
     1861 acttccaccc ccaccggctc tatatgcgct tcctcgaccc catcacccca caacccggag
     1921 acacggtcga aacccttaaa caacaggtat ttaaacttat gtgggaaaac tatacggcag
     1981 gtcctcagac ccggccctga cccccggcca caaactcccc ggccgcaaac cggaccgtcg
     2041 caactgcaaa ctccgcaacc gcaaactccc cggcccccaa ctccccggcg gcaaaccgcg
     2101 ccgcggcact cctactgatc ataaaaatac ttcgtccggt tgatccgcag gtcctgcaat
     2161 atccccgcct tcgggttgat cgtgatattg aaataatggc tgtaccccac cggcgtcaca
     2221 ttgatcgaca tctgccagca atgcaggtcc cgcgaaatat tcatcgtcag cgactgcagc
     2281 ttcgtatacc gcaggtcata gtacccgttc aacccgatct tccacttatc ggtgaggttg
     2341 aaatccccgc tccagttcag gctcgaggtg atcgtcgtct gaaacccgct gtaatccggc
     2401 ttctctgcat tcgtgaaatt gaacgccagc gacaggttca acgaccatgg gatattgaaa
     2461 tccgcatact gcgccgaatt gttccgcata tagttcaact gcgcctgctg ctcctccatc
     2521 gtcatcggca tcgcattctg gctgttcatc agctgctgct tctgctgctc cagcttctgg
     2581 tccttcggct tgcttttgaa actggtggag atcgccacat tccccgtcgt gatccgcccc
     2641 agcgagaact tcttcgcatc ccaggcatac ttgttcctgt caaaacccat cgaatcggtc
     2701 tgatacgggt ccagcgtagc ccccccggtg atattgatgc tgttgaataa tgtgctccgg
     2761 aagtagaagg taatcggcga taacttgaac gaatccgcca ggtagttata gcttccgttg
     2821 aacccaaagc catcgataag cgtgatcttc ttcgaagccg aagtgctcgt atccgacttg
     2881 ttccgcgtct tgatctcgat atggttatcc agcccgaagg agataccccc aaaccggcct
     2941 tccccaaaag gtccgtaggt cgatccgtcg aagtacgaca cccgctgcac atgcgatagc
     3001 ttcgacgccc ccgccgtcgt gtcataaggc gtcgtcaggt tataataata cggacccgac
     3061 aggtcgggct tataactgat actgatcgta ggccggatca catgccggat ccccatcagg
     3121 gtgctgttcc tgccaaaatt ctggaaggta ccaaagatag ccgtggcgag gctcaggctg
     3181 aacgacatat cgttcgcata aaataccccc ttctgaatgc cgacggtatc caccttgttg
     3241 gagatggggt catacttccg gaacagcttc cgcgaataca aacggttttg caggctgatc
     3301 cccggcgaca cctgcaccgg ccccttcaac ggcaacgcca ggctcagcgg gatactgttc
     3361 tgcgcccccc actggaaggt atccgttatc cgcttcaggc tgaaaagcga gtcataaaaa
     3421 ctcgccccgc ccgcgatagt ggtgctcagc ccgatgccga acttctcata ccatttcggg
     3481 gtcccgacaa aggtgctccg ggcaaacggg tagatcgtcg gcgcggtaaa agcgatattg
     3541 ggcaggctca cgttcacgat cttt
//
LOCUS       DATGFG010001129         3949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_622398, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001129 DATGFG010000000
VERSION     DATGFG010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622398"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VKQ52_21390"
     CDS             complement(<1..440)
                     /locus_tag="VKQ52_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89828.1"
                     /translation="MTTTTPTFSTRQGLSTRKKVLWLVGLLLALLCGPYIAVYLQYGG
                     IPDKFFNFPATTADGQKPAPTLFMTVGITIVFIAALLLLLFPRLFGFKKAPPVARTAK
                     RKARATLPPWFWVGLIASAGTLVFTGMHIRKPVWLNDWALLPLW"
     gene            complement(559..1683)
                     /locus_tag="VKQ52_21395"
     CDS             complement(559..1683)
                     /locus_tag="VKQ52_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89829.1"
                     /translation="MTNLDLVEPNTKSESLSLYQPLLIRHPEGGHDPKDVLPLSEFIA
                     LLKEEEDYWQGEQANLPVMITRLRKIFYDKWGWNKDLIRRAADIECRYLTTITDTPPT
                     DSSGQSHVRRTRHYKNNNEVEKYRLVTYRKDDRVFGDTRAGQIPFIYQKDHQEVLLPD
                     GTYCDIAHVLAGLDAYNNPQIVSPLPPWLEFLHDIVPHCDSNMDLVTWLGDIATSCEA
                     FVFDYLRHNKKPVSARAEQHYIDVNAPGSDMLGNIDSYAIARSYDLSGASGRRLTDIL
                     EEYYTGPAKSLFAQRRYTLFCDAVGLKWDGQKFANEEEWIKRYRPQLRDATTFMIFSL
                     TEENLQSVLLPLEVWLGGYSDVAKCEILLRLFLRALKALI"
     gene            1732..2400
                     /locus_tag="VKQ52_21400"
     CDS             1732..2400
                     /locus_tag="VKQ52_21400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ89830.1"
                     /translation="MDKRKPSVALVDDHVMLRAGLANIIRNFESHEVLFEADNGHQCL
                     DKLQAGLVPSIVLLDINMPGMDGYATALAIRNRFPEVKVLALSMYDSEDSIIRMLRNG
                     ARGYILKDSDPSELFAALQDIHEKGYYHSEMISSRLIRLVNDLDNPDGQLKQMVTLNE
                     REIEFLKLSCTELTYKEIADKMGVSPRTVDGYRDILFEKLPVKTRVGLVLYAIRSGLV
                     HVRE"
     gene            complement(2704..>3949)
                     /locus_tag="VKQ52_21405"
     CDS             complement(2704..>3949)
                     /locus_tag="VKQ52_21405"
                     /inference="COORDINATES: protein motif:HMM:NF027421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HLZ89831.1"
                     /translation="VKICMNNHITDPQMQEVLAGFLHDLGVIVRFKGLNLSNVYILQP
                     KWVSQAAYKIINSKKIADNHGVLLKDDLNVILRKEREGDFYFPLHFFSYILTLLERFE
                     ICFALSEDKYLLPELLDIQQPKLPIQHGECIRFYFEYESLLPSSIFTRFVVRMNQDIW
                     NNLCWRTGVVLAVDVFSSTAIIVVDPKERRIHISVDGEGRREHFAIIRKTFHDLHNSF
                     EKLKVVEMIALPVENKFIAYSELVAYNKHGKEEYFSGELERNFNVKEILDTIEPENTR
                     TRKYQWDVFLIHASADKRLISAITDDLKAKKITYWLDDEQLMPGDDLLEGIKQGLMNS
                     GVLIPCLSRNQKRSNWTLTEYSSILNRMISGYSDQRVAPLVLDNMPEEDIDIFLASRK
                     WIRYQDKDQYGKLLDFLTRKKW"
BASE COUNT          846 a          910 c         1194 g          999 t
ORIGIN      
        1 caccagaggg gcaggagggc ccaatcgttc agccagacgg gtttgcggat atgcatgccg
       61 gtgaagacga gggtcccggc ggaggcgatg aggccgaccc agaaccaggg tggcagggtg
      121 gcccgggcct ttctttttgc cgtcctggcg acggggggcg cttttttgaa tccaaagaga
      181 cgcgggaaga ggagaagcag tagggctgcg atgaacacga tcgtgatacc cacggtcatg
      241 aacagggtag gagcgggttt ctgcccgtcg gcggtggtag cgggaaagtt gaagaacttg
      301 tccgggatgc cgccgtattg gagatagacg gcgatatagg ggccgcagag cagggccagc
      361 agcaggccga cgagccaaag cacttttttc cgggtgctca gcccttgccg cgtgctgaag
      421 gttggggtgg tggtggtcat agttcgggtg gttatgggtg ttaggtgatg aagttcaggt
      481 aatggttttt cgggcggatg tggttattcg gacggatgct gtttttcggg tggatgctgt
      541 cgttcgggtg gaggccgttc aaatgagcgc cttcagggcc cggaggaaga ggcgcagcag
      601 gatctcacac ttggcgacat cgctatagcc gcccagccat acttctaggg gcaggaggac
      661 gctttgcagg ttctcctcgg tgaggctaaa gatcataaag gtggtggcat cgcgcagctg
      721 cggccggtaa cgtttgatcc attcttcttc gttggcgaat ttctggccgt cccatttcag
      781 gccgacggcg tcgcagaaca gggtatagcg tcgttgggcg aagagagatt ttgccggacc
      841 ggtatagtat tcctccagaa tgtcggtgag gcgtctgccg ctggcgccgg agaggtcgta
      901 gctccgggcg atggcgtagg agtcgatatt gcccagcata tccgaaccgg gggcgttgac
      961 gtcgatataa tgttgttccg ccctggctga caccggtttt ttattgtggc ggagatagtc
     1021 gaagacaaag gcttcgcaag aagtggcgat atcgcccagc caggtcacga ggtccatgtt
     1081 cgagtcgcag tgcggtacga tgtcgtggag gaattcgagc caggggggta ggggtgatac
     1141 gatctgcgga ttattatagg cgtcgagccc ggccaggaca tgagcgatgt cgcaataggt
     1201 tccgtcgggc agcagcactt cctggtgatc cttctggtag atgaagggta tctggccggc
     1261 gcgggtatcg ccgaagacgc ggtcgtcctt gcggtaggtg acgagccggt atttctcgac
     1321 ctcgttgtta tttttataat gtcttgtcct gcggacatgg ctttgcccgg aactgtccgt
     1381 gggcggggta tcggtgatgg tagtgaggta gcggcattcg atgtcggcgg cgcggcggat
     1441 gagatccttg ttccagcccc atttatcgta gaagatcttt cgcaggcggg tgatcatgac
     1501 cggcaggttg gcctgttcac cctgccagta gtcctcttct tcttttaaaa gagcgatgaa
     1561 ctcgctgagc gggaggacgt ctttgggatc gtggccccct tcgggatggc ggatcaggag
     1621 gggctggtaa agcgacaacg attcgctttt ggtgttcggt tcgacaaggt ccaggttggt
     1681 catactttat tttttcacgg ttattaatta actttcggga taacctatca tatggacaag
     1741 cgcaaaccct cggtagcatt agtagatgat catgtgatgc tccgggccgg attggccaat
     1801 atcatccgga attttgaaag ccacgaagtc cttttcgagg ctgacaacgg tcatcaatgc
     1861 ctggataaat tgcaggcagg gcttgttcct tccattgttc tgctcgatat caatatgccc
     1921 gggatggatg gctatgccac cgccctggcg atcaggaacc ggtttcccga ggtaaaagtg
     1981 cttgccttgt ccatgtatga ttccgaggat tcgatcatcc ggatgctgcg caacggagcc
     2041 agggggtata tcctgaagga cagcgatccg tcggagctgt ttgcggccct gcaggacatc
     2101 cacgagaagg gctattatca ttcggagatg atcagcagcc ggctgatccg cctggtgaac
     2161 gacctggaca atcccgatgg gcagctgaag caaatggtca ccctcaatga aagggagatc
     2221 gaattcctca agctctcgtg taccgaatta acctataagg agattgcgga caagatgggt
     2281 gtcagtcccc gcacggtgga tgggtaccgg gatattcttt ttgagaagct tccggtaaag
     2341 acaagggtag gactggtcct ctacgctatc cgtagcggac tggtgcatgt gcgcgagtag
     2401 acctgccgtc tccggcggct cctggcgggg cgatgaaggt gttcacgcgc atgttggttt
     2461 aaggatcagg ggatggaagg tttaatggca atggactctg ataagggcag ccccagacga
     2521 tcggcgggtt tggcggccgg ttggcagcgc ctgtcgatca cctgtttgta ttcggggagc
     2581 taagtaagga taattgattg gtttcccgta ccgtgtttta acggtgggaa tggggatttt
     2641 acacggtggg ggggggtgga tctgagagcg aaatataacc tgacgggata aaatacagtt
     2701 ttgctaccat ttctttcttg tcaagaaatc cagcagtttc ccatactggt ccttatcctg
     2761 ataccgtatc catttcctgg atgccaggaa aatgtcgata tcctcttccg gcatattgtc
     2821 gaggaccagt ggcgccactc gctggtcgga atagccgctg atcatgcggt tcaggatgct
     2881 ggaatattcg gtaagggtcc agttcgatct tttttggttt cggctgagac aaggaatgag
     2941 cacgccggaa ttcatcaatc cctgctttat gccttcgaga aggtcgtccc ctggcattaa
     3001 ttgttcatcg tccagccaat aggttatctt tttggcctta agatcatcgg ttatcgcgct
     3061 tatcaggcgc ttgtcggcag aagcgtgaat aaggaagaca tcccattgat atttccgggt
     3121 tcgggtattc tccggttcga tcgtgtccaa tatctctttg acattaaaat tcctttccaa
     3181 ttccccactg aagtattcct ccttcccatg cttgttgtac gccacgagtt cggaataggc
     3241 gatgaatttg ttctccaccg gaagggcgat catttccact acctttagtt tctcgaagct
     3301 gttgtgcaga tcgtgaaatg tttttcggat gatggcaaaa tgttcccgcc tgccctcccc
     3361 gtcgacactg atatggatgc gcctttcttt tgggtccacg acgatgatcg ccgtcgacga
     3421 aaatacgtct accgccagca ctactccagt gcgccagcag aggttattcc agatgtcctg
     3481 gttcattcgg acgacgaacc gggtaaaaat ggagctgggt aacaggctct catattcaaa
     3541 ataaaacctg atgcattcgc cgtgttgaat cggtagtttt ggctgttgga tatccaataa
     3601 ttccggaagc aggtacttat cttcggacaa ggcgaagcaa atttcgaatc tttccaggag
     3661 tgtgaggata tacgaaaaga aatgcaacgg aaagtaaaag tcaccctccc gctcttttct
     3721 caggatgaca ttcagatcat cctttaacag gaccccatga ttgtcggcga tctttttcga
     3781 attgatgatc ttataggctg cttgtgagac ccacttaggc tgcaggatat agacgttgga
     3841 aaggttgagg cctttgaagc ggacgatgac cccaaggtcg tgtagaaaac cggccagtac
     3901 ctcctgcatc tgagggtcgg tgatatggtt attcatacat atcttgaca
//
LOCUS       DATGFG010001130         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_411735, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001130 DATGFG010000000
VERSION     DATGFG010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411735"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VKQ52_21410"
     CDS             complement(<1..556)
                     /locus_tag="VKQ52_21410"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HLZ89832.1"
                     /translation="MGLSTTKEIKKKAKPTPRPTNGAAKTVSRVKTYESDALLDDKDL
                     LRILLQVRNGNFGVRMPIGEVGVKGRIYDTLNEIIILNEEMMREFTKAGNTIGKQGKL
                     TQRIEVPNAKGSWATGVDSLNALISDLVHPTIEIAHVISSVAKGNLSQNMPLEIGGHT
                     LQGEFSRIAREVNDMVKQLNLFSME"
     gene            693..1139
                     /locus_tag="VKQ52_21415"
     CDS             693..1139
                     /locus_tag="VKQ52_21415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLZ89833.1"
                     /translation="MKSNTRLIYIVDDDPDDRQIILDAFLEKSPQIDYVFIENAETLM
                     ETLYASEAELPALILLDLNMPGMLGLHALKEIRSNRQFSQIPVIVLTTSTLQQDRKTS
                     YELGASCFLRKPDSFTELVDITDSIVKLWLHEYQPQPQSSPPAGKF"
     gene            1256..1594
                     /locus_tag="VKQ52_21420"
     CDS             1256..1594
                     /locus_tag="VKQ52_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89834.1"
                     /translation="MKTSKIIVLPALLFFTLRGLGEGLNTVSRPVDLPSKEVIATMTE
                     EQKQALVQQMKDRVEYIKSMDKSALSKDERKALRTELKGMKKEGRVITGVYISVGALI
                     IIILLLIIII"
     gene            1779..>2818
                     /locus_tag="VKQ52_21425"
     CDS             1779..>2818
                     /locus_tag="VKQ52_21425"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HLZ89835.1"
                     /translation="MELAIPATANTAAAGAAITGTTPAPAGAAAITARLPFIDNLRWV
                     MIMLVLSMHAAVTYSGHGSWYYNEPTHLNRGEEMLFVTYQVFLQSFFMGLLFFVAGYF
                     VPGAFDKKGGRRFLRDRAWRLGMPSLLYIFVLHPITNYYAARNWAADDPHRSFLREYA
                     HYITRGHFPGGSGPLWFCLALLFFCCVYAAWRTLTGSASGNSALTPRKASTTPRSFPR
                     AAGIIAFIALIAAATFLVRIPWPNGTSFYNMQFCYFPQYIAFFIAGTLAYRHSWLTTL
                     PTAAGKRWGRIGLFGGLVLWIALIILGGALKGQAAAYGGGWHWQSLGMSILESMAGVG
                     LSLGCLSLFREK"
BASE COUNT          602 a          949 c          649 g          618 t
ORIGIN      
        1 cctccatgga aaagaggttc agctgcttca ccatatcatt cacctcccgc gcgatccgcg
       61 agaactcccc ctgcagggtg tgcccaccga tctccagcgg catattctgc gacaggttgc
      121 ccttcgccac cgaactgatc acatgcgcga tctcgatcgt gggatgcacg agatcggaga
      181 taagggcatt gagagaatcc acccccgtcg cccagctccc cttggcattg ggtacctcga
      241 tccgctgcgt aagtttcccc tgcttgccga tcgtatttcc cgccttcgta aattcccgca
      301 tcatctcttc attcaggata atgatctcgt tcagcgtgtc atagatgcgg cccttaacac
      361 ccacctcacc aatgggcatg cgcacgccga aattcccgtt ccgaacctgc aggagtatgc
      421 ggagcaggtc cttatcatcc aaaagcgcgt cagattcgta tgtttttacc cgggaaacgg
      481 tcttggcggc tccgttggtc ggacgagggg ttggcttggc tttctttttg atttccttag
      541 tggtggatag tcccatattg gaattttact ttacccaact accccacaat ccccgcgcca
      601 tatatttggc aaggttctgg tgtaaggata tccagcaccc ttaaagacac aacagcctgg
      661 ccttatcacc gcttagaacc acatttttaa ttatgaaaag taacactcga cttatctaca
      721 tcgtcgacga tgatccggat gataggcaga ttattttgga tgctttcctc gaaaaaagtc
      781 cgcagatcga ctacgtcttt atcgagaatg cggagacctt aatggaaacc ctgtatgcgt
      841 ccgaggccga gttgccagcc cttatcctgc tcgatctcaa catgccaggc atgctgggat
      901 tacatgcgct caaggagatc cgcagcaacc ggcaattcag ccagatcccg gtcatcgtgc
      961 tgaccacatc caccctgcaa caggaccgga aaacgtccta cgaattgggc gccagctgtt
     1021 ttcttcgaaa accggattct ttcaccgaat tggtggacat caccgattcc atcgtaaaac
     1081 tctggttaca cgagtaccaa ccacaacccc aatcttcccc gccggcagga aaattttagc
     1141 ataatcataa aaaagtgtgg agtttattcc acactttttg ttaatagcca gatgactggc
     1201 cgggaatggc atttgagcta aataaggcag gaagcacatc acaaaatctt ttaacatgaa
     1261 aacaagtaag atcatagtcc tgcccgccct gctcttcttt acgctgcgcg ggcttggcga
     1321 gggtctcaac acggtctcaa gaccggtgga cctgccctcc aaagaggtga tcgccaccat
     1381 gaccgaagaa caaaaacagg cgcttgttca acagatgaag gacagggtgg aatacataaa
     1441 gtcgatggac aaatcagcgc tgtcaaagga cgaacgaaag gccctgcgga cggagctgaa
     1501 gggcatgaaa aaagaaggta gggtcatcac cggggtctat atctccgtcg gcgccctgat
     1561 catcatcatc ctgctgctga tcatcatcat ctaatacctt tacacctctc ccccacaggc
     1621 cggttcccgc aaggggccgg ccattctttt gccgggacgc ccgtccaccc cgcaaatctc
     1681 cggacgccca tcttttcgcc accaatcccc gcccatacgt caaagaaact tgcgccccgc
     1741 cctcccgcac actagtttta tgccacaatc aaccaaacat ggaactagcc atccccgcca
     1801 ccgccaacac cgccgcagca ggcgcagcca tcaccggcac aacccccgcc cctgcgggcg
     1861 ccgccgccat caccgcccgc ctgcccttca tcgataacct ccgctgggtc atgatcatgc
     1921 tcgtcctcag catgcacgcc gccgtcacct acagcggtca cggcagctgg tattacaacg
     1981 agcctaccca cctcaaccgc ggcgaagaaa tgctcttcgt cacctaccag gtcttcctcc
     2041 agtccttttt catgggcctg ctcttcttcg tcgccggcta cttcgtaccc ggagccttcg
     2101 acaaaaaagg cggccgccgc ttccttcgcg accgcgcctg gcgcctgggc atgccctccc
     2161 tgttgtatat cttcgtcctc caccccatca ccaattacta tgcggcccgc aactgggccg
     2221 ccgatgaccc ccatcgcagc ttcctgcgcg aatacgcgca ttatattacc cggggccact
     2281 tccccggcgg ctccggcccg ctctggttct gcctggcgct gctcttcttc tgctgcgttt
     2341 acgccgcctg gcgcaccctc accgggtcag cttccggaaa ctccgccctc acccctcgta
     2401 aagcatccac caccccccgg tccttccccc gcgccgccgg tatcatcgcc tttatcgccc
     2461 tcatcgccgc cgccaccttc ctggtgcgca tcccctggcc aaacgggaca tcgttctaca
     2521 atatgcagtt ctgctatttc ccgcaataca tcgccttctt catcgccggc accctcgcgt
     2581 atcgccatag ctggctcacg accctgccga cagcggccgg caaacgctgg ggccgcatcg
     2641 ggctctttgg cggcctcgtc ctctggatcg ccctgatcat cctcggcggc gccctgaaag
     2701 gccaggcagc ggcctacggc ggcggctggc actggcaaag cctcgggatg tccatcctcg
     2761 aatcgatggc gggcgtcggc ctctccctcg gctgcctcag cctcttccgc gagaaatt
//
LOCUS       DATGFG010001131         5297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_868326, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001131 DATGFG010000000
VERSION     DATGFG010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868326"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VKQ52_21430"
     CDS             complement(<1..567)
                     /locus_tag="VKQ52_21430"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HLZ89836.1"
                     /translation="MSTFFLLLILLFGFLITFQIAKASEYVGVLRGEKKNFQQHNRIN
                     GFLMIVFLVLGLIGVYYCNELYRGKILGESASKHGEEIDRMLYITIAITGVVFVITQI
                     LLFWFAYKYQYSEKRKAHYFPHDNRLEVIWTVVPAIALTVLVGFGLFYWFRITGEAPQ
                     DALQIEVTGKQFGWIFRYPGHDGTFGKKY"
     gene            complement(584..1810)
                     /locus_tag="VKQ52_21435"
     CDS             complement(584..1810)
                     /locus_tag="VKQ52_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89837.1"
                     /translation="MSSIKEHFEIPGRYKKWSMGLMGVGVLSLIIGFALYGTHDQGTR
                     FWASLLQNSVYFLLVVNAAMFFFAATTLAMAGFQLTFRRVTEAIAASVIPIGIITFVI
                     LMCLVFGGKTTLYEWLDRDVVAKDAALNGKKGFLSVGFFTTWTILTIGLWTFLGWKLR
                     QISRSIDNTPLTVQEGKKYTFKNTVWAALFIVWFGLTVASVTPWLWLMSLNAHWFSTM
                     YSWYTFASTFVAGLALITIFVIYVKNQGYLEYVNEEHLHDLGKFMFAFSIFWTYLWFD
                     QFMLIWYANISEETVYFKPRFEGAYAGIFYLNLIINFLCPLLIFMSRGSKRNYATVTF
                     MSVLLIFGHWLDFYQMVFPAQHISPDHVPLNLFDFGVALGFVGLIMWQTGRVLSTYPL
                     LAKNHPFLKESIIHHT"
     gene            complement(1863..2513)
                     /locus_tag="VKQ52_21440"
     CDS             complement(1863..2513)
                     /locus_tag="VKQ52_21440"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLZ89838.1"
                     /translation="MKKLLILSFIVVTVTVLISCGGVQREPGRVYMPDMGYSRAYETY
                     SMTEEQKAALLKQGIHFSNTPVAGTIKRGELLPFLLTKDQPGDSTNYVASKVVKNPLT
                     SMDTVEAERLYLVNCGICHGPKLDGNGPLYKGGDGPFPSKPATLVGDPRYEAMPEGQM
                     YYSVTYGKNKMGSYASQLSTTRRWMVIAYIKSKQAAAKGGSAGSGSSAGADSTAKK"
     gene            complement(2536..3126)
                     /locus_tag="VKQ52_21445"
     CDS             complement(2536..3126)
                     /locus_tag="VKQ52_21445"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HLZ89839.1"
                     /translation="MAVKKFVVGIFTDEAVLFPAIKKVRSAGYKIHDVYTPMPIHGMD
                     HALGLRETSLHTAGFIYGITGTTTALSGIGWVFNSDWPVNIGGKSHFDLPAWIPITFE
                     LTVLFSAVGMFLTFLYICQMAPFVRKHHFNLRSTDDQFTMVIELTEKSNEESVKAFLQ
                     GAGATGINTQVAETGWWLGRYDKDKTPFEHKGATLA"
     gene            complement(3140..4591)
                     /gene="nrfD"
                     /locus_tag="VKQ52_21450"
     CDS             complement(3140..4591)
                     /gene="nrfD"
                     /locus_tag="VKQ52_21450"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HLZ89840.1"
                     /translation="MSSPLRYESQVRTALVEGQKDYHQVTEDIVRPIEAAPSRLWWIG
                     FLISVACLLFGVWSVTEQVIYGVGQWNLHRTIGWGWDITNFVWWVGIGHAGTLISAIL
                     LLFRQGWRTGVNRAAEAMTIFAVMCAGQFPIWHMGRVWLCFFIMPYPNTRGPLWPNFN
                     SPLLWDVFAISTYFTVSLLFWYSGLIPDIATVRDRARVKWRKLMYGVLSFGWTGSTKH
                     WQRHESLSLVLAGLSTPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAM
                     VQTLLLVSRKVLKLEQYITLEHIEVMNKVIVLTGSIVGIAYLTELFISWYGQNKFESW
                     AFAQNRVNIFSPYGWAYYLMMGCNVLSPQIFWFRKMRRNLTVTFFMSVIVNIGMWFER
                     FVIIVTSIYRDYLPSSWATYYAPSIWEVGFYVGTFGLFFTCYFLFSKFFPVIAIAEIK
                     HILKKSGESYKEKMDVEERMPVEEFAHQYAHAH"
     gene            complement(4626..>5297)
                     /locus_tag="VKQ52_21455"
     CDS             complement(4626..>5297)
                     /locus_tag="VKQ52_21455"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLZ89841.1"
                     /translation="DSPEDVQVVFQPMLCQHCDNAPCENVCPVNATNHSTEGLNQMAY
                     NRCIGTRYCANNCPYKVRRFNWADYTGADSFPNNQDQSVVGKLDPVIFQMNDDLTRMV
                     LNPDVVVRSRGVMEKCSFCVQRLQDGKLKAKKENRVLEDMVDVQTACQQACPTDAIVF
                     GNVNSSKTRISTTRAEHADRLYYVIEELHTLPNVSYLAKVRHTEAAFGAAEEKTEEAA
                     AEKHS"
BASE COUNT         1330 a         1420 c         1385 g         1162 t
ORIGIN      
        1 atattttttg ccgaaagttc catcatgacc cgggtaacgg aagatccagc cgaactgctt
       61 gcccgtgacc tctatctgca gcgcatcctg cggagcctca cccgtgatac ggaaccagta
      121 gaacaggcca aagcccacga ggacagtcag cgcgatcgcc ggaacgacgg tccagatcac
      181 ctcgagacgg ttgtcatgcg ggaagtaatg cgcctttctt ttctcggaat attggtactt
      241 ataggcaaac cagaagagca aaatctgcgt gatgacaaac acgacgcccg tgatggcgat
      301 ggtgatatac agcatcctgt cgatctcctc gccatgcttg gaagcggact cgccaaggat
      361 cttgcccctg tagagctcgt tgcaatagta gaccccgata aggcccagca cgaggaagac
      421 gatcatgagg aaaccgttga tacggttatg ctgctggaaa ttctttttct ctccccttag
      481 tacaccaaca tactcacttg cctttgcaat ctgaaaggtg atcaggaagc caaacaacag
      541 gatcagtaac agaaaaaaag ttgacatgat agttaaacaa gttttaagta tgatggataa
      601 tactttcttt caggaaagga tggttttttg ccagcagcgg atacgtgctc agcactcttc
      661 cggtctgcca catgatcaat cctacaaaac ccagcgcgac accgaagtcg aagagattca
      721 ggggcacatg gtcgggggag atatgttgtg ccgggaagac catctggtaa aaatccagcc
      781 agtgaccaaa gatcaaaagt acggacataa aggttacagt ggcatagttc cgcttggatc
      841 cgcggctcat aaaaatcagc agcgggcaca aaaagttgat tatcaaattc aaatagaaga
      901 tgccggcata cgctccttca aaacggggct tgaaataaac agtctcctcg gagatgttcg
      961 cataccagat gagcatgaac tggtcgaacc agagataggt ccagaagata gaaaaggcga
     1021 acatgaactt accaaggtcg tgcagatgct cctcgttgac atactccagg tagccctggt
     1081 tcttgacgta gatgacaaag atggtgatca gcgccaaccc ggcaacaaag gtactggcaa
     1141 aggtgtacca gctgtacatg gtagagaacc agtgggcatt gaggctcatc agccacagcc
     1201 agggagtcac ggatgcgacg gtgagaccga accacacgat aaacagcgcc gcccagaccg
     1261 tattcttgaa ggtatatttt ttcccttcct gcaccgtgag aggagtatta tcgatgctgc
     1321 gggagatttg gcgaagcttc cagcccagga aggtccacag gccgatggtc aggatcgtcc
     1381 aggtcgtgaa aaagccgacg ctgaggaaac cctttttccc gttcagggcg gcgtcctttg
     1441 cgacgacatc acggtccagc cattcgtaga gggtcgtctt gccgccgaag accaggcaca
     1501 tgaggatcac gaaggtgatg atcccgatcg ggatcaccga tgcagcgatg gcttcggtaa
     1561 cccggcggaa agtcagctga aagccggcca tcgccagggt agtggcggca aagaagaaca
     1621 tggccgcatt cactaccagc aggaagtaga cactgttctg cagcagggag gcccagaaac
     1681 gggtaccttg atcgtgggtg ccgtagagcg caaatccaat gatcagggag agtaccccaa
     1741 cgcccattaa tcccattgac catttcttat agcggccggg tatctcaaaa tgttctttaa
     1801 tggatgacat tcaacaatga tttaatgtat tcgttaatcc tttttcttct ctgctgcagg
     1861 ttttattttt tcgcggttga atcggctccg gcggaagacc cgctcccggc gctgccgccc
     1921 ttggccgctg cttgtttcga ttttatgtac gcgatcacca tccagcgtct tgtagtgctc
     1981 agctgtgatg cataactgcc cattttgttc ttgccatacg taaccgagta gtacatctgt
     2041 ccttcaggca tggcctcata ccgcggatca cctaccaggg tcgcgggctt cgaggggaag
     2101 ggaccgtcgc cgcccttgta aagcggaccg ttgccgtcca gcttggggcc atgacagatc
     2161 ccgcagttca ccaggtaaag ccgctcggcc tctaccgtgt ccatcgaggt caacgggttc
     2221 ttcaccacct tcgaggccac ataattggta ctgtcgcccg gctggtcctt ggtaagcagg
     2281 aagggcagga gctctccgcg cttgatcgta ccggcaaccg gcgtattgct gaaatggatc
     2341 ccctgcttca gaagcgccgc cttctgctct tcggtcatag aataggtctc atatgcccgg
     2401 ctatagccca tgtcaggcat atagactctt ccgggttcgc gctgaacgcc accacaactg
     2461 atgaggacgg tcaccgtaac aactataaac gataagatga gtagtttttt catgataata
     2521 ttcttagact gtcgcttagg ctaaagttgc tcctttgtgt tcaaatgggg ttttgtcctt
     2581 gtcatagcgg cccagccacc agcccgtctc ggccacctgg gtgttgatcc ccgtcgcacc
     2641 ggcgccctgc aggaaagcct ttaccgactc ctcattgctc ttctccgtca gctcgatcac
     2701 catcgtgaac tggtcatccg tagaccggag attgaaatga tgcttgcgga cgaacggcgc
     2761 catctggcag atataaagga aggtcaggaa catcccgacg gcagagaaca aaacagtcag
     2821 ttcgaacgta ataggaatcc acgccggcag gtcgaagtgc gatttgccgc cgatattgac
     2881 cggccaatcg ctattgaaga cccaaccgat gccgctcagc gcggtggtcg tacccgtgat
     2941 cccataaata aagccggccg tatggaggct ggtctccctc agacccaacg catggtccat
     3001 cccgtggatc ggcatgggcg tatagacgtc atggatctta tatcccgcgg aacgaacctt
     3061 cttgatcgca ggaaacagta ccgcttcgtc cgtaaaaatg ccaactacaa actttttaac
     3121 tgccatactt atttagcttt taatgcgcat gtgcgtattg gtgtgcaaat tcttcgacgg
     3181 gcatccgctc ttccacatcc atcttctcct tgtaggattc gccggacttc ttcaggatat
     3241 gcttgatctc ggcaatggcg atcaccggga agaatttaga gaacaggaaa taacaggtga
     3301 agaacaaccc gaacgtaccc acatagaacc cgacctccca aatgctcggc gcatagtaag
     3361 tggcccagct gctgggaaga tagtcccgat agatggaggt aacgatgatc acaaaccgtt
     3421 cgaaccacat cccgatattg acgatcacgg acatgaagaa agtgacggtc agattgcgcc
     3481 gcatcttgcg gaaccagaag atctggggcg acaacacgtt acaacccatc atcaggtaat
     3541 aggcccagcc ataggggcta aaaatattca cccggttttg ggcaaaggcc cagctttcga
     3601 acttattctg accgtaccag gagatgaaca attccgtcag gtaggcgata ccgacgatcg
     3661 aacccgtgag aacaattacc ttgttcatca cctcgatatg ttccagggta atatattgtt
     3721 ccagcttcag caccttgcgg ctgacaagca gcagggtctg caccatcgca aaaccggaga
     3781 agatggcgcc cgcaacgaag taaggaggga agatcgtggt atgccagccc gggataacgg
     3841 aggtggcgaa gtcaaaggac acgatcgtgt gcaccgaaag tacaagcggc gtactcaaac
     3901 cggccaacac cagggacagc gactcatgcc gttgccagtg cttggtcgaa cccgtccagc
     3961 caaaggacaa tacgccatac atcaatttcc gccatttcac tctcgcgcgg tcgcggaccg
     4021 tggcgatatc cgggatcaga cccgagtacc agaagagcaa cgatacggtg aaataagtag
     4081 agatcgcgaa cacgtcccag agaaggggag agttgaagtt cggccagaga ggaccccggg
     4141 tattgggata aggcatgata aagaagcaca accagacacg acccatatgc cagatcggga
     4201 actgcccggc gcacataacg gcaaagatgg tcatggcctc agccgccctg ttcacacccg
     4261 tacgccagcc ctgacgaaac agcagaagga tcgcggagat aagcgtcccg gcgtgaccga
     4321 taccgaccca ccagacgaag ttcgtgatat cccagcccca gccgatggtc ctgtgaaggt
     4381 tccactggcc gacaccatag atcacttgct cggtgacgga ccagacaccg aataaaagac
     4441 aggccaccga gatcaggaag ccgatccacc acagacggga gggagcggct tcgatcggcc
     4501 gaacaatatc ttccgtaacc tggtgataat ctttctgacc ttcaaccagc gctgttctta
     4561 cttgtgattc gtaccttaag ggagatgaca tagtatgttg agctttttgc tattgatatt
     4621 gttgactacg aatgcttttc tgctgctgct tcttccgttt tctcttcggc tgcgccaaaa
     4681 gcggcttccg tatgacgaac cttggccagg tagctgacat tgggtaacgt atgcaactct
     4741 tcgataacgt agtacagccg gtcggcatgc tcggcacggg tcgtactgat ccgtgtcttg
     4801 ctgctgttga cattgccgaa cacgatggcg tccgtggggc aagcctgctg gcaggccgtt
     4861 tgcacatcca ccatgtcttc gagcacccgg ttctctttct ttgcctttaa cttaccatcc
     4921 tgcagacgct gtacgcagaa actgcacttt tccatgacgc cacggctgcg gacaacgaca
     4981 tcggggttta gcaccatccg ggtcagatcg tcattcatct ggaagatgac cggatccaat
     5041 tttccgacga ctgactgatc ctggttgttc gggaagctgt cggcgcccgt gtagtcggcc
     5101 cagttgaagc gacggacttt gtacggacag ttgtttgcgc agtagcgggt accgatacac
     5161 cggttgtacg ccatctggtt cagcccttcc gtgctatggt tggtggcatt caccgggcaa
     5221 acgttctcac agggggcatt gtcgcagtgc tggcaaagca tcggctggaa cacgacctgt
     5281 acgtcctcgg ggctgtc
//
LOCUS       DATGFG010001132         5308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_14082, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001132 DATGFG010000000
VERSION     DATGFG010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14082"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2066)
                     /gene="ccsA"
                     /locus_tag="VKQ52_21460"
     CDS             complement(<1..2066)
                     /gene="ccsA"
                     /locus_tag="VKQ52_21460"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLZ89842.1"
                     /translation="MEYLGEHLWPGRLGHFFALLSFAASFVAMIAYFKATRAKAPEEQ
                     NSWRRMGRIAFCVDVFAVFAVLATIFYIVSGRLFEYNFAWEHSSRALRFQYVLACIWE
                     AQEGSFLLWNMWVCVLGLILVRTAGKWESPVMTVMSFTQFCLATMIMGIYLFGNRVGL
                     NPFILVRQTEMGLRAPIFSQPDYLSIPQMQDGQGLNALLQNYWMVIHPPVLFLGFSST
                     VVPFAYAIAGLWKKDFGGWTRVALPWTLFSACILGTGVMMGAAWAYEALSFGGYWAWD
                     PVENASMVPWLVIVAGLHTQVIYNATGHSLRATYFFLITQFILVLYSTFLTRSGILGD
                     LSVHAFVSAGMNVQLASFLLIFLIPAYSLFIARYKRIPNIAKEEATNSREFWMFIGAL
                     ILFLSAAYVIISTSLPVINLVREKKVSIDDAPFTYNRIEVFIAILIGIFTAFAQYLKY
                     KGTGKGVLIQKLWLPTVIALAISVPISIWGGINYDKYGVGFLAAIHLALFAAIYTVVA
                     NAAYIRTVLKGNMRSAGGSVAHIGFGLMLVGILISSARKQVLSYNNEGVRFANADPKQ
                     DPLENLTLVKGIRMEMKKFWVTYVGNDSLDDRSQTRYYHLHFQDKQTADQFDLYPNMM
                     KNTKGQEGGSPNPDKFHYWNRDIFTYITGFSPEQKDEDTAQFQPQAVKLKDTAFYSKG
                     YVILDSV"
     gene            complement(2077..2490)
                     /locus_tag="VKQ52_21465"
     CDS             complement(2077..2490)
                     /locus_tag="VKQ52_21465"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HLZ89843.1"
                     /translation="MKKLHIVILILIAVSIAVLVSFTGDLTTFETIASARQKSGKTVT
                     IIAHLDNSQPIEYNPARDPNYLSFHISDTLGNKAKVVYHFEKPYDLEKAERITLKGKM
                     NGDVFEITRKDGILLKCPSKYKDDPNNAKRALSQN"
     gene            complement(2841..4082)
                     /gene="fabF"
                     /locus_tag="VKQ52_21470"
     CDS             complement(2841..4082)
                     /gene="fabF"
                     /locus_tag="VKQ52_21470"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLZ89844.1"
                     /translation="MKRVVVTGIGAVTPLGNDPVTYWRQLVAGVSGAGPITHFDASQF
                     KTRFACEVRGFAPGALLDRNELKRTDLFTQYALVAADQAIKDCGIDFKAMDPFDTGVI
                     WGSGQGGMETFEEQVKEYAGGDGTPRFSPFFVPKLIANMASGMISIKYGFMGINYTTV
                     SACSTSNTAFMDALTYIRLGKAKVMVAGGSEAPITAASVGGFTAMKAMSTRNDDPQHA
                     SRPFDVDRDGFVMGEGGAALILEEYEHAKARGARIYGEFAGAAMTADAYHMTHTHPEG
                     AGAFRAMQDALKDAGINKEEVDYLNVHATSTPVGDLSEVQAVTRLFGTAPSRLVISAT
                     KSMTGHLLGAAGAIEAIACLMSIQDGVIPPTINTQHLDPAIPAGLNILLGEAKKQPVN
                     VAMSNTFGFGGHNGIVVFKKI"
     gene            complement(4109..4726)
                     /locus_tag="VKQ52_21475"
     CDS             complement(4109..4726)
                     /locus_tag="VKQ52_21475"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLZ89845.1"
                     /translation="MSATREHIIDLADKVIREKGFNAFPYADIAAVLEIRKAAIHYHF
                     PSKSQLGQAVMEEELSRLDLFRRLNARVGGDLQLKHLVGTFYHNARRQVVCLMGALTP
                     EFATFDAEMQATLRRLCLVIREWVGECLEEARVGERLRFEGPAMDRAALVVSTLLASL
                     LLSRIEGVEIFRQMVDRMLEDLGAHWRVGDLAEADPSVDRHYSFT"
BASE COUNT         1180 a         1811 c         1208 g         1109 t
ORIGIN      
        1 accgagtcga gaatgacata tcctttcgaa tagaacgcag tatcttttag cttcaccgcc
       61 tgtggttgga attgcgcggt atcctcgtcc ttctgttcgg gtgagaatcc ggtgatatag
      121 gtgaagatat ccctgttcca gtaatggaac ttatcagggt tcggcgaacc gccctcctgt
      181 cccttcgtat tcttcatcat gttggggtag agatcgaact ggtccgccgt ctgcttatcc
      241 tggaaatgca ggtggtagta gcgggtctga ctgcgatcat caagcgaatc attcccgaca
      301 taggtcaccc agaatttctt catctccatc cggatgccct tcaccagggt caggttctcc
      361 aacgggtcct gcttgggatc cgcattcgca aaacggacac cctcgttatt ataggaaagc
      421 acctgtttcc gcgccgaaga gataaggatc cccaccagca tcaacccaaa accgatatgt
      481 gcaaccgaac cgccagccga tcgcatattg cccttcagca ccgtacggat atacgccgca
      541 ttggccacca cagtatagat cgcggcaaaa agcgcgagat gtatcgccgc caggaaaccc
      601 acgccatact tgtcataatt tatcccgccc cagatgctga tcggcaccga gatcgccagc
      661 gcgatcacgg tcggcagcca caatttttga atcagcaccc ccttgcccgt tcccttatac
      721 ttcaggtatt gggcaaaagc cgtaaagatc ccgatcagaa tggcaataaa gacctcgatc
      781 ctgttatagg taaacggtgc atcgtcgata ctcaccttct tctcccggac gaggttgatc
      841 actggcaacg aagtggagat gatgacatac gccgccgaaa ggaagaggat cagtgcaccg
      901 atgaacatcc agaactcacg ggaattggtg gcctcctcct tcgcaatgtt gggtatccgc
      961 ttatatctgg cgataaacaa cgaataggca ggaataagaa agatcagcag gaaagaagcc
     1021 agttgcacat tcatcccagc actcacaaac gcatgcaccg agaggtcgcc aaggatcccg
     1081 ctccgcgtga ggaaggtaga gtataacacc aggatgaact gcgtgatcag gaagaaatag
     1141 gtcgcccgca gggaatgccc cgtggcgtta taaatcacct gggtatgcaa ccctgccaca
     1201 atcaccagcc aggggaccat cgaggcgttc tccaccgggt cccaggccca gtaaccgcca
     1261 aaactaagcg cttcataagc ccacgcggcc cccatcatca cgcccgttcc aagaatgcag
     1321 gcagaaaaca gcgtccaggg caacgcgacc cgcgtccagc cgccaaaatc ctttttccaa
     1381 agcccggcga tggcatacgc aaacggcacc accgtcgatg agaacccaag gaaaagcacc
     1441 ggcggatgga tcaccatcca ataattttgt aaaagggcat tgagcccctg gccgtcctgc
     1501 atctgcggaa tgctgagata gtccggctgg ctgaagatcg gcgcccgcag gcccatctcc
     1561 gtttgccgga caagaatgaa gggattgagc cccacccggt tgccgaaaag ataaatgccc
     1621 atgatcatcg tcgccaggca gaattgcgta aagctcatca ccgtcatcac cggtgactcc
     1681 catttgcccg ccgtacgcac gaggatcagc cccagcacac agacccacat attccacagc
     1741 aggaagctgc cttcctgcgc ttcccagatg caggccagca catattggaa tcgcaaagcc
     1801 cggctcgaat gctcccaggc aaaattgtat tcgaaaagcc ggcctgagac gatatagaag
     1861 atggttgcaa ggacagcaaa gacggcaaaa acatccacac aaaaagcaat acgacccatc
     1921 cgccgccaac tgttctgctc ttcgggggcc ttcgcccggg tcgccttgaa ataagcgatc
     1981 atggcgacaa aagaagctgc aaaggaaaga agggcaaaga aatgaccaag cctgccgggc
     2041 caaagatgtt cgccgaggta ttccatttcc ggggtgttaa ttttggctca gcgctcgctt
     2101 cgcattattg ggatcgtcct tgtacttgga aggacatttc agcaggatac catccttgcg
     2161 ggtgatctcg aagacatcac cgttcatctt tcccttcagc gtgatccgct ccgccttctc
     2221 caggtcgtag ggcttctcga aatgatagac caccttggcc ttgttgccaa gcgtatcgct
     2281 gatatgaaaa ctgagataat tcggatccct ggcaggattg tactctatag gctgggaatt
     2341 gtcgaggtgc gcaatgatcg taacggtctt gccgctcttc tgccgcgcgg aagcaatcgt
     2401 ctcaaaggtg gtcagatcac cggtgaaact caccaggacg gcaatcgaga cggcaatgag
     2461 gatcagtatg acgatatgta gtttcttcat aacagagtgc aaagttaggc caaaaagcag
     2521 ttcgccgtaa tagattctgg ttatcagccc atcgatcgtt ccaaaccccg cctttccatc
     2581 cgggccgccc gggtcttttc ctcctgtccc gctcatcccg tcccgctcct tcagcgctgc
     2641 ctatcctcaa caatcgtttc ctcctatcaa atccggctcg ccctggcctt tgccgcccct
     2701 ctccttcagc gctgcttatc ctgctcaatc ggttcctcct gtcaaatccg gcccgccccg
     2761 gcctttccgt cctgtccttc tcacccgtcc ctctcgcccg gttcttccca tccaacttct
     2821 tctccgcccg cccgggaaac ctagatcttc ttgaaaacaa cgatcccgtt gtgcccccca
     2881 aacccaaagg tattgctcat tgccacgttg accggctgtt tcttcgcctc cccgagcagg
     2941 atattcagcc cggcggggat cgccgggtcc agatgttggg tattgatcgt cggagggatc
     3001 accccatcct ggatgctcat cagacacgca atcgcctcga tggcgcccgc cgcacccagc
     3061 aaatgccccg tcatggactt tgttgcactg atgacaagcc gggacggcgc cgtcccaaaa
     3121 agccgggtca ccgcctgcac ctcgctaaga tcacccacgg gcgtcgacgt tgcatggaca
     3181 ttcaggtaat cgacctcttc tttattgata ccggcgtcct tcaacgcatc ctgcatggcc
     3241 cggaaagccc cggccccctc gggatgcgtg tgggtcatat gataggcgtc ggccgtcatc
     3301 gcggcgccgg caaactcgcc atagatccgc gcaccccttg ccttcgcatg ttcgtactct
     3361 tcaaggataa gcgccgcgcc gccctcgccc atcacaaaac catcccggtc tacatcgaaa
     3421 ggccgggaag catgctgggg atcgtcattg cgcgtcgaca tcgccttcat cgccgtaaat
     3481 ccacccaccg acgcggccgt gatcggcgct tccgatccac ccgcaaccat cactttagcc
     3541 ttccccaacc ggatataggt gagcgcatcc ataaaggccg tgttggacgt actacacgcc
     3601 gacacggtag tataattgat ccccataaaa ccatatttga tggagatcat ccccgacgcc
     3661 atattcgcga tcagcttggg tacgaaaaag gggctaaaac ggggtgtccc gtcaccaccg
     3721 gcatattcct tcacctgttc ttcgaaggtc tccatcccac cctggcccga accccagatc
     3781 acacccgtat cgaatgggtc catcgccttg aagtcgatgc cgcaatcctt gatggcctgg
     3841 tcggccgcga caagcgcgta ctgggtaaaa agatcggtcc gcttcagctc attgcggtcc
     3901 agcagggcgc cgggggcaaa gccacggacc tcgcaggcaa acctcgtctt gaactggctg
     3961 gcatcgaaat gggtgatcgg ccccgccccg ctcacgccgg caaccagctg ccgccaatac
     4021 gtaacaggat cgtttcccag cggcgtaacc gcaccaatac cggtaacaac tactcttttc
     4081 ataattactt tttgatcttt cctttcgatc aggtgaatga ataatgacga tccacactgg
     4141 gatcggcttc cgccagatcc ccaacccgcc aatgcgcacc aaggtcttcc agcatccggt
     4201 cgaccatctg ccggaaaatc tccactccct ctatccgtga taacaacaag gaggccaata
     4261 atgtcgacac caccaacgcc gcccggtcca tggccgggcc ctcgaaccgc agccgctccc
     4321 caaccctcgc ctcctccagg cattccccca cccattcccg gatgacaagg cacagcctcc
     4381 gcaacgtcgc ctgcatctcc gcatcaaagg tcgcaaattc cggggtaagc gcccccatca
     4441 ggcaaaccac ctggcggcgg gcattatgat agaaggtgcc gaccaggtgt ttcaactgca
     4501 aatctccgcc cacccgggca ttcagacgcc ggaacagatc cagccgcgaa agctcctctt
     4561 ccatcaccgc ctgcccaagc tgcgacttgc tgggaaaatg atagtggatg gccgccttgc
     4621 ggatctccag cacagcggca atatccgcat acggaaaggc attaaacccc ttctcccgga
     4681 tcaccttatc cgccaggtca ataatatgtt cacgcgtcgc actcatttcg catccctcgg
     4741 ccgccttccg ttccggtatc gctctcaacc tctcccaacc cctcccggct ttcccggccc
     4801 ggtgtttccg gctccccggt tctcccggct ttcccggccc ggtgtctccg gctccccggt
     4861 tctcccggcc tacccggccc ccgggtttct ctggctcccc ggttccccgc cctgcccggc
     4921 tcctcacggc tttccccgcc ctccggttcc cccggcgccc ccacaaatct acttacttac
     4981 tagtaagtaa gcaaatttca catcgccgtc cccccgcatc cgccgcctcc tcgtccacgc
     5041 cccctggttc cactccatcc acacgctacc catgccctct ccccctcccc cgatatctac
     5101 ctcaccccct tcacccctcc cgctccctcc aactcctcga actcccttct cccctcctac
     5161 tccctccaac tcctcgaact cccttcaccc ctcctactcc cctccaactc cgcggactcc
     5221 ctttccccct cccactcctc ctactccccc tccctcaaac ttcctcctac ttctccccac
     5281 tccctccaac ttccatcacc ccatccac
//
LOCUS       DATGFG010001133         4875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1555649, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001133 DATGFG010000000
VERSION     DATGFG010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555649"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VKQ52_21480"
     CDS             complement(<1..849)
                     /locus_tag="VKQ52_21480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89846.1"
                     /translation="MLSNYGKLALRNLRRNKVFSTINILGLSVGLAACLLITAWVRDE
                     ESYDRYAYRSKDIYRVNLGVNTATQSEYPMVDIAVGPGMAAAYPDIETFTRLGNLGDA
                     YIRYGTRNFKESHLVYVDSNFFEVFTVPFLEGDAHTALLRPNSLVVTRAFVTKYFGRE
                     PALGKTVDLSNYGACIITGIIDKIPDEAHFHFDAFLSWSSKHFSQYSWTNIGFYTYLR
                     LRPDADPAKLQARFPELVAKYCVPEISRDMGVPLAEARKAVNSFVFSLTPVADIHLHS
                     NTKYEIE"
     gene            1055..1414
                     /locus_tag="VKQ52_21485"
     CDS             1055..1414
                     /locus_tag="VKQ52_21485"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLZ89847.1"
                     /translation="MSTTEIANRLVEICSRGEFEAAQKELYADDAVSIEPHGTQDFAQ
                     ETKGLNAIIEKGKKWAEMVSEYHGMKVSQPLVGENTFAVTMWMDVTMKDRGRMAMSEL
                     CVYHVKDGKIVSEQFFM"
     gene            complement(1526..2710)
                     /locus_tag="VKQ52_21490"
     CDS             complement(1526..2710)
                     /locus_tag="VKQ52_21490"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HLZ89848.1"
                     /translation="MKRLLFISGVLLLFATEILRVYFIMPFPGSQHADTIGLAYFIDR
                     YRILLRTVGVLLVIFPLLSYWRRPKRWPRVWLTVLILFYGVIFYFFNFRFEADKMFYQ
                     PGTKTFATAAVNTLPPGRLVIGVALNGEAKAYPIQIIGYHHQVKDTIGNTPVIITYCT
                     VCRTGRVFSPLVNGKAETFRLVGMDHFNAMFEDATTQSWWQQATGVAIAGPLKDNHLT
                     EIPSAQSSLAQWLQQYPDSKILQPDTAFNKQYDALADYDKGTIKGSLERRDSGSWKAK
                     SWVVGILSGGKARAYDWNELVSHPLIEDTLGGLPIAVVLGADSVTFHGWSRRIGGQDL
                     SFEKLPGQGLLKDTNTGSTWNSNGLCIEGKLKDQQLAALPAYQEFWHSWSNFHPSTSR
                     YR"
     gene            complement(2767..3453)
                     /locus_tag="VKQ52_21495"
     CDS             complement(2767..3453)
                     /locus_tag="VKQ52_21495"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89849.1"
                     /translation="MLKHIPAIAIACFFTLSAQAQDCLHYFFMQKDKTVEMTIYNKKG
                     EPSGRQVYQVSDVASSGGTTTAKLNSEMFDKKGKSIAKSNSSIQCNGGALLIDMKLML
                     PQQTAEQTGGAETQANAQTSYIEYPSSMKVGDALHDASFAMDIKRGGMTQTVTMQITE
                     RKVLAQESVTTPAGTWDCFKISEKSRVSVKTGPIGIPFTVESTEWYAPSFGVVKSESK
                     NGGTAITSIK"
     gene            complement(3519..3959)
                     /locus_tag="VKQ52_21500"
     CDS             complement(3519..3959)
                     /locus_tag="VKQ52_21500"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLZ89850.1"
                     /translation="MRELDSYDKHLLRLLQQNSRLTADELSAAVNLSASAVQRRLKKL
                     REDKIIEAEIAIVNPEALGIGISCVVDIILQDGNSSAVEGFRSTMRKCPEVSHCYYVT
                     GTYDFVIIVHVKDMRQYEEFSKKRLMDNPIVKHFYTHVVIDRVS"
     gene            4079..>4875
                     /locus_tag="VKQ52_21505"
     CDS             4079..>4875
                     /locus_tag="VKQ52_21505"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLZ89851.1"
                     /translation="MKQEEIARYRADTRGCNNVIHLNNAGAGLMPDGVTRALQEHLQL
                     ESEIGGYEAAAMRADAVAQFYVQAGKLLNCRPENIAFGCSATDAYTRALSSIPFRPGD
                     IVLIANDDYISNQIQLLSCQKRLGIRLERIRNASIGGVDLQDLGEKLHRLRPRLLAIT
                     HIPTSSGLVQPVEAIADIYARYIAAHEGDTWYLLDACQSAGQLKLDVEALKCDFLSVT
                     ARKFLRGPRGAGFLYISDKALQHGLEPLFLDMRGAEWVEKDVYRQRPT"
BASE COUNT         1092 a         1299 c         1399 g         1085 t
ORIGIN      
        1 ctctatttcg tatttggtgt tcgaatgaag atggatatcc gccaccggcg tcagcgaaaa
       61 aacaaagctg ttgaccgcct tcctggcctc ggccagcggc acgcccatgt cgcgggagat
      121 ctcgggaaca cagtatttgg cgacgagctc ggggaaacgg gcctggagct tcgcggggtc
      181 ggcatcgggc cgaagccgga ggtaggtgta aaagcctata ttggtccagc tgtactggct
      241 gaaatgcttc gaggaccagc tgagaaaggc atcgaaatgg aaatgggcct catcggggat
      301 cttgtcgatg atcccggtga tgatacaggc gccgtaattg gagagatcga cggtcttgcc
      361 cagggcgggc tccctgccga agtatttagt gacaaaggcc cgggttacga cgaggctgtt
      421 gggccgcagg agcgcggtgt gggcgtcgcc ctcgagaaag gggacggtga acacctcgaa
      481 gaaattgctg tcgacataga caagatgcga ttccttgaaa ttccgggtcc cgtatcggat
      541 ataggcgtca cccaggttgc cgagccgggt gaaggtctcg atgtcggggt aggcggcggc
      601 catgcccggc ccgacggcaa tatccaccat cggatactcc gactgggtgg cggtattgac
      661 acccaggttg acccggtaga tatccttcga ccggtaagca taacggtcat aactttcctc
      721 gtccctgacc caggcggtga tcaggagaca ggcggcaagc ccgacggaaa gacctaggat
      781 attgatggtc gaaaatacct tgttgcgccg gagattgcgc agggcgagtt taccgtagtt
      841 gctgagcatg agggttcgtt tggcggtgta ccggtcaggg accaagaaaa atgccagttg
      901 tctaaagttc tgataatcaa atattttttc cgagatctcg cgacgggctg ttcgtttttg
      961 gtacgctcgg tgtgcgaaat gttaatatgg cggggcccgg aatttgcgct aactttccta
     1021 aaacactttt tttatgtcaa caacaactgc agtcatgtca acgacagaga tcgccaatcg
     1081 cctggtcgag atctgtagcc ggggcgaatt cgaggccgca cagaaggagc tttatgcaga
     1141 cgacgccgtc agcatcgagc ctcatggaac ccaggatttt gcacaggaaa caaaggggtt
     1201 gaacgccatc atcgaaaaag gaaagaaatg ggcggaaatg gtatcggagt atcacgggat
     1261 gaaagtatca cagccgctgg ttggggagaa caccttcgcg gtaaccatgt ggatggatgt
     1321 gacgatgaaa gatcgcggac gcatggcgat gtccgaactt tgcgtgtatc atgtcaagga
     1381 cggaaaaatt gtatcggagc aattctttat gtaacggaat gagcagatcg atgcgtcaat
     1441 cctggaactt accggcgatg ctcaaccgca tcgccttttt ctttgtctta cccggccgcg
     1501 tctattttga ccggcttatt cacccctacc ggtaccggct ggtcgaagga tggaaattgc
     1561 tccaggagtg ccagaattcc tggtaagccg gcagcgcagc caactgctga tcctttagct
     1621 tcccttcgat gcaaagccca ttgctgttcc aggtggaccc cgtgttggta tccttgagca
     1681 ggccctggcc gggaagcttc tcaaacgaca ggtcctgtcc ccctatccgg cggctccagc
     1741 cgtggaaggt gacggaatcc gcgcccagca cgacggcgat cggcaggcca ccaagcgtgt
     1801 cctcgatcag cggatggctg accaactcat tccagtcata ggcccgggcc ttacctcccg
     1861 agaggatccc cacgacccag gactttgcct tccatgaccc ggagtccctc ctttccaggc
     1921 ttcccttgat ggtgccttta tcatagtctg caagtgcgtc gtactgtttg ttgaatgcgg
     1981 tatcgggttg caggatcttc gagtcgggat attgctggag ccactgcgca agcgaagact
     2041 gcgccgaagg tatctcggta aggtggttat ccttcaacgg ccccgcaatg gccactcccg
     2101 tcgcctgctg ccaccagctt tgggtagtgg catcctcgaa catggcattg aaatggtcca
     2161 tgcccacgag ccggaaggtt tcagccttgc cgttgacaag cggactgaaa acccggccgg
     2221 tgcggcaaac ggtgcaatag gtgatgatca ccggggtatt gccgatagta tccttcacct
     2281 ggtggtgata accgatgatc tggatggggt aagccttcgc ctccccgttg agggctacgc
     2341 cgatgacgag ccggcccggc gggagggtat tgacggccgc cgtggcaaag gtcttggtgc
     2401 ccggctggta gaacatttta tcggcttcaa accggaaatt gaaaaaatag aagatgacgc
     2461 cataaaagag gatcagcacc gtaagccata ccctgggcca gcgtttgggg cgccgccagt
     2521 agcttagcag cggaaagatc accagcaata ccccgacggt ccgcaggagg atgcggtagc
     2581 ggtcgataaa ataggccagc ccgatggtat cggcatgctg gctgccgggg aaaggcatga
     2641 taaaatatac ccgcaggatc tcggtcgcga acaggagaag gacgccggag ataaagagca
     2701 gtcgtttcat tacactaata taatcatttg ggccggcaaa acttggggcc ggcaaaagcc
     2761 ccccgctcat tttatcgagg tgatcgccgt cccgccattc ttactctcgc tcttgacgac
     2821 gccaaaggag ggcgcatacc attccgtgct ctcgacggtg aacggtatac cgatggggcc
     2881 cgtcttcacc gagacacggc tcttttcgct gatcttgaaa caatcccagg tcccggcagg
     2941 cgttgtaacg gattcctggg ccagcacctt cctctccgtg atctgcatgg tcacggtctg
     3001 ggtcatccca ccccttttta tatccatcgc aaaactggca tcgtgcagcg catcgcccac
     3061 cttcatggaa gaggggtatt cgatatagct ggtctgcgcg ttcgcctggg tctcggcgcc
     3121 cccggtttgc tcggcggttt gctggggcag cataagcttc atgtcgatga gcagggcgcc
     3181 gccgttgcat tggatggagc tgttactttt cgcaatggac tttccctttt tgtcgaacat
     3241 ttcggagttg agcttggccg ttgtggttcc gccgctgctg gcgacgtcgg acacctggta
     3301 cacctgcctg ccgctgggct ctcccttctt gttgtagatc gtcatttcga cggtcttgtc
     3361 cttctgcatg aagaagtaat gcaggcagtc ctgggcttgg gcagaaaggg taaaaaagca
     3421 ggcaatggca atcgccggaa tgtgtttaag catggtagtg atggatttgc ggtaagatag
     3481 ggactatttc ggggccccgc aataccacaa accgatgatt aacttaccct atcaatgact
     3541 acatgggtat aaaaatgctt tacaatgggg ttgtccatca gccgtttctt ggaaaactcc
     3601 tcatattgac gcatgtcttt tacatggaca atgatcacga aatcataggt gcccgtaaca
     3661 taataacagt gcgacacctc ggggcatttt ctcatcgtgg atcggaatcc ctctacggcg
     3721 ctggagttgc catcctgtaa gatgatgtcg acaacacaac tgatcccgat gcccagcgct
     3781 tcagggttga cgatagcaat ctctgcttcg atgatcttat cttcccgaag ctttttcaac
     3841 cggcgttgta cggcggaggc gcttaggttg accgctgcgg acaattcatc ggcggtgagc
     3901 cggctatttt gctgtaacag cctgagcagg tgcttgtcat acgaatccaa ttcccgcata
     3961 atttacccgg ttttggggtt aaaactacct aaaaaacccg ctaatgactg ttctttgccg
     4021 aaaaaatgcg attcgtcgcg cctagttttg ccctgtaata ctaaaaagaa aaacgaccat
     4081 gaaacaagaa gagatcgccc ggtacagggc cgacacaagg ggctgtaaca atgtcatcca
     4141 tttgaacaat gccggcgccg gcctgatgcc ggatggcgtg acccgcgcct tgcaggaaca
     4201 cctgcaactg gagtcggaga tcggaggcta tgaggccgcg gcgatgaggg ccgacgccgt
     4261 ggcgcaattt tatgtacagg cgggcaaact gctgaactgc cgcccggaga atatcgcctt
     4321 cgggtgcagc gcaaccgatg cgtatacacg ggccctttcc tctatcccct tccggccggg
     4381 agatatcgta ctcatcgcca acgatgatta tatttccaat cagatccagc tcctttcctg
     4441 tcagaagcgg ttgggcatcc gcctcgaacg gatccggaac gcctccatcg ggggagtaga
     4501 cctgcaggac ctcggggaga aactccaccg cctgcgaccc cggctcctgg ctatcaccca
     4561 tatccccacc agttcgggac tggttcaacc ggtagaggcg atcgcggata tctatgcccg
     4621 gtatatcgcc gcacacgagg gcgatacatg gtatctcctc gatgcctgtc aatccgccgg
     4681 tcagctaaag ctggacgtgg aagctttgaa atgcgacttc ctgagcgtta cggcccggaa
     4741 attcttgcgg ggaccacggg gagcggggtt cctgtatatc tcggacaagg ccctgcagca
     4801 cgggctcgaa cccctcttcc tcgatatgcg tggcgccgaa tgggtggaga aggacgtata
     4861 ccggcaacga cctac
//
LOCUS       DATGFG010001134         5589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_868583, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001134 DATGFG010000000
VERSION     DATGFG010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868583"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VKQ52_21510"
     CDS             complement(<1..350)
                     /locus_tag="VKQ52_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89852.1"
                     /translation="MKKLLICRLRRYFPFFSVLLSLCSVSVAQTPDTALFGNLRWRMI
                     GPHRGGRTVGGCGIPQQPNVFYIGVNNGGVWKTTDFGRTWTPVFDSQPTGSIGDVAVA
                     PSDPNIVYVASGEG"
     gene            complement(444..1718)
                     /locus_tag="VKQ52_21515"
     CDS             complement(444..1718)
                     /locus_tag="VKQ52_21515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLZ89853.1"
                     /translation="MLLQDDASPGQLERAAAINHTTLFRQESVALGGNIITTTTGEFS
                     FTSGTVHSPSMIPFPELQPEEAGKQLDELVAFYLQHPPKGAGCWSLEPSLPADLGVRL
                     LARGFQPGWRPRWMGLDLHQVKTGHPAPKGLSIVADNTLSLDSIRELPYAQVIVPPPA
                     LAELPGQWMRFIARWKGKVVAHSVVFLSTGPYGAAGIYHVGVVPRARHMGIGKAVTLA
                     ACLYARERGYRYAVLNSTDVGRRTYEQLGFVTVGEGWTWWLLTDRLLSRPPTPKTVLL
                     AEAIGRGDREALDGMRPLFGPADLDRPLTNGMTLMQLAVHCRQEAGGEWLVENGAAYS
                     VLDAWDMDWKDRARELITSNPASVNQVYGEWDKTLLHFAAERNDEELARLALSAGPNL
                     QLKDKAYQGTALSWAQFFGRGAIVQMIEACRR"
     gene            complement(1784..2068)
                     /locus_tag="VKQ52_21520"
     CDS             complement(1784..2068)
                     /locus_tag="VKQ52_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89854.1"
                     /translation="MITRIEAEEEDLSILHLRRGFPEAFQVFVFEYYAELFSFSLLLV
                     NDRSSARKITSEAFFLLWARRTEFDTAKKIKAFLYLAVRNRCMLQVRSAD"
     gene            complement(2080..2460)
                     /locus_tag="VKQ52_21525"
     CDS             complement(2080..2460)
                     /locus_tag="VKQ52_21525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89855.1"
                     /translation="MSGFRLKLNRLKNIPYASSIQEHLPFEKTFEVEYFFDGRWSLVL
                     VHPILVNTVVIFLVSFPEGEAESMALVYNKKDIWADIEKESTGLTEAVGASIENYYSE
                     CYLPWSGSFGDGNTNPYWDSHLLN"
     gene            2770..3381
                     /locus_tag="VKQ52_21530"
     CDS             2770..3381
                     /locus_tag="VKQ52_21530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ89856.1"
                     /translation="MQPQILLADDHSMIRKGLKLLLQLHLGFTEVSEVVSCNLLMKEL
                     GRKKYSHLVLDIILSDGSTLEILPNIRSLYPELRIMVFSMQPAEVYGKALRQYGIEHY
                     LPKTTPEEETIRLLRRFLLNEQTVRDNPDVRYPNNPFSALAPRELEILHYVLKGLGTK
                     EIAETLNVKMNTVSTVKNRIFEKTLTTNLKELIELATLYNVNY"
     gene            3368..>5589
                     /locus_tag="VKQ52_21535"
     CDS             3368..>5589
                     /locus_tag="VKQ52_21535"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HLZ89857.1"
                     /translation="MSTTKITGLSCWLLGCCLSLAAQSNYHKRTGGPPAEGQTLAENG
                     RLANFQTAGLPFSIQHFSTDNGLPSNGIKGLQWDEKTGFLWIATEAGVARYNGADFQV
                     FNKASSPEMYSERMLFLLRTRDGRIYTSDETGDLFFVMQNKLQFVGNVKVDTRPSTFK
                     LTGRVASGRLFRQSSQQPPADFGFNWFQEQLVPLDESRLLLTHDDTLYDYRAGQPLPL
                     KIAPLEQHSRIFYLDRNLFVFHPAHGFYRISPDSPKKIPVELEGWEKIKEKPILAWDN
                     GMPAPLLVSGSNVWTLSYNGAKLQSRLICTALPRDALLAFLKYDSASGLLFVGTQSRG
                     ILIIRKNEVRSVRKTLPAPEQPTAYYSQLALPGGEFLSGKGDQLGGGPNSPAVLAMPG
                     TFNNFIFTDPDSNCWYSHGDTVYTFSYRTHRSRAFAAGSGSITDGFAWSEGHLYIANA
                     IGVGMLGGEGIDYQYRYPQANINSNVPFAMVEINPGAIAIATCNGLFRYNIAGHRLDT
                     LLRIPGICVRALWKYKGYLLIGTYGRGIWVYRDGAIRPIPPDKKGYLLYAHCFVPDGL
                     GYCWISTNRGLFRARPDDMTEAFDKDLREVYYHYYGREDGMDMTELNGGCTPCALPLN
                     DTILSFPSMDGLVWVNPQGPIPTLPEGSIYIDAFMADSQRVNITSLVKPLLPASTRDL
                     HFQLAFPAWVNKENLNIEYQLEPWSKDWENLDLTHGPELHFSNLPYGEYRLLVRKADG
                     P"
BASE COUNT         1284 a         1610 c         1521 g         1174 t
ORIGIN      
        1 atcccctcgc cgctcgccac atagacgatg ttcgggtcgg aaggcgcgac ggcgacatcc
       61 ccgatggacc ccgtgggttg ggaatcgaat accggcgtcc atgtccggcc gaaatcggtc
      121 gttttccata cgcctccgtt attgacgccg atgtaaaaga catttggttg ttgcggtatc
      181 ccgcaaccgc ccaccgtcct gcccccccgg tgcggaccga tcatccgcca gcgcaggttg
      241 ccgaacaggg cggtgtcggg agtctgcgcc accgaaacgg aacaaaggga aagcaatacg
      301 gaaaagaagg ggaaataccg gcgaagccgg cagataagca gttttttcat gtgtagcttt
      361 tgtagaagga gaaaaattac gacataatgg tttcttcccc ggaaccgtcc atcaactttt
      421 tttaatcggt ggatggaaat ttcctaccgc cggcaggcct cgatcatctg cacgatggcg
      481 ccgcggccaa aaaattgcgc ccagctcagg gccgtgccct ggtaggcctt gtctttcagc
      541 tggagattgg ggcccgcgga aagcgccagc cgggcaagtt cctcatcgtt gcgttcggcg
      601 gcaaaatgca ggagggtctt atcccattca ccgtatacct ggttcaccga tgccgggttg
      661 ctggtgatca gctcacgtgc acggtccttc cagtccatat cccaggcatc cagcaccgaa
      721 taagcggcgc cgttctctac cagccattct ccgcctgcct cctgccggca atgcacggcc
      781 agctgcatca gggtcatccc attggtaagc ggccggtcga gatcggcggg accaaacagc
      841 ggccgcatgc cgtcaagcgc ttcgcggtcg ccgcgaccga tggcctcggc cagcaatacc
      901 gtctttggcg tgggcgggcg cgacaggaga cggtcggtga gcagccacca ggtccatccc
      961 tcgccgacgg tgacaaaccc cagctgttcg taggttcgcc ggcccacatc ggtggagttc
     1021 aggacggcat accggtatcc cctttcccgg gcataaagac aggcggcaag ggtcaccgcc
     1081 ttgccgatcc ccatatgccg ggcgcgcggg acgaccccga catgatagat cccggcggcg
     1141 ccatagggac ccgtgctaag aaagacgacg ctgtgcgcga ccacctttcc cttccatctg
     1201 gcgataaagc gcatccactg ccccggtagt tccgccaatg ccggaggagg tacgatcacc
     1261 tgggcatagg gcagctccct gatgctatcg agggatagcg tgttatcggc gacgatactc
     1321 aggcctttcg gcgcgggatg cccggtcttc acctggtgga ggtcgagccc catccaacgg
     1381 gggcgccagc cgggttgaaa accccgggcc agcaaccgga cacccaggtc ggcgggaagg
     1441 gagggttcaa gcgaccagca acccgcaccc tttggcggat gttgcaggta aaaggccacc
     1501 agttcatcca attgcttccc ggcctcctcc ggctgcagct cggggaaagg gatcatcgaa
     1561 ggactgtgca ccgttccgct ggtgaaggaa aactcccccg ttgtggtggt aatgatattc
     1621 ccgccaagcg ccacggattc ctgccggaac agggtcgtgt gattaatggc tgccgctcgt
     1681 tctaattgac ctgggctggc gtcgtcttgc aatagcatgg gatgagtctt tcgaagcaat
     1741 ctcgctgacc ctgaaaataa ggaaaaaaag acaagttggg atatcaatcg gcagacctga
     1801 cctgcagcat gcaccggttg cgtacggcca gatagagaaa ggccttgatc tttttggcgg
     1861 tgtcgaactc cgtgcgtctg gcccagagga gaaaaaaggc ttccgacgtg atcttccggg
     1921 cggaagaacg atcgttcacc agcaacagcg agaaggaaaa aagctcggca taatattcaa
     1981 aaacgaagac ctggaacgct tccggaaatc cacggcgcaa atgcaggata ctaagatcct
     2041 cttcttctgc ttctatcctc gtgatcatat ggaagcggct taattcagga gatgactgtc
     2101 ccagtatggg ttggtattgc cgtcaccaaa gctgcccgac cagggaaggt aacattcgga
     2161 ataatagttc tcgatggagg cgcccacggc ttcggtcagt cccgtggatt ccttttcgat
     2221 atcggcccag atatccttct tattatatac aagcgccatg gactcggcct ccccttcggg
     2281 aaagctcacg agaaagatga ccaccgtatt gacaaggatc ggatggacga gcaccaggct
     2341 ccatctgccg tcgaagaaat attccacctc gaaggtcttt tcaaacggga ggtgctcctg
     2401 gatcgaagaa gcatatggaa tattttttaa cctgttgagt tttaagcgaa aacctgacat
     2461 agttataaat taaagtatga agccctttgt ttggtcgaaa caggactggc aggtcataaa
     2521 gtgatggtca aaaactttga gcttaacagg atactgcagg acacaaattg cgattgccgt
     2581 gtacaaactg cttgatctgg atcagcattt gattaatggt gatcaaatac gcttagcaaa
     2641 ttaatagctt tacgctatcg aaacgtagaa ttaattctac agggcattac tttttaatga
     2701 tgaatttagc attattgctc ctcttaggag aaaacactta ttgaatttac cggcctgtcc
     2761 catacaacta tgcaaccaca gatattactg gcagatgacc acagcatgat ccggaaaggc
     2821 ctgaaactat tgctgcaatt gcatcttggg tttacggagg tatccgaagt ggtcagctgc
     2881 aacctgctga tgaaggagct gggcaggaag aaatactccc acctggtttt ggacatcatt
     2941 ttgtcggatg ggagcacact cgagatcctg cccaatatcc ggagcctgta tccggagctt
     3001 cggatcatgg tcttctccat gcaacccgcg gaagtctacg gaaaggccct gcggcagtac
     3061 gggatcgagc attacctgcc gaagaccacg cccgaagaag agacgatccg cctgctgcgc
     3121 cgcttcctgc tgaatgaaca aaccgtccgc gacaacccgg acgtgcgtta tcccaacaat
     3181 cctttttcgg ccctggcacc ccgtgagctg gagatcctcc actatgtcct gaagggattg
     3241 gggacaaaag agatcgccga aacgctcaac gtaaagatga atacggtatc caccgtcaag
     3301 aatcgtatct tcgagaaaac cctcacgaca aacctgaaag aattgataga attggctacc
     3361 ttgtacaatg tcaactacta agataacagg tttatcttgc tggctgctcg gctgttgcct
     3421 ttcccttgcg gcgcagtcaa attatcacaa gcggaccgga ggtccgccgg ccgaaggcca
     3481 gacattagcg gaaaacggga gactcgctaa tttccagact gcgggtctcc cgttttcaat
     3541 tcaacatttc tccaccgaca acgggcttcc ttccaacggc atcaagggtt tgcaatggga
     3601 tgaaaagacg ggatttctct ggatcgccac ggaagccggc gtcgcccgtt acaatggcgc
     3661 cgatttccag gtctttaata aggccagctc cccggagatg tattcggaaa ggatgctctt
     3721 cctgctccgg acccgggatg gccggatcta tacctcggac gagaccggtg atcttttctt
     3781 tgtcatgcag aacaagctgc aattcgtcgg caacgtcaag gtcgacaccc gccccagcac
     3841 attcaagctg acggggcggg tcgcatccgg ccggctgttc cgccaatcct cgcagcagcc
     3901 gcccgccgat ttcgggttca actggttcca ggaacaactg gtaccactgg acgaaagtcg
     3961 gctcctgctg acgcatgacg ataccctgta cgattaccgg gcggggcaac cccttccctt
     4021 gaagatcgcg cccctggaac agcattcgag gatcttctat ctcgaccgta acctgtttgt
     4081 ctttcatccg gcccatggct tctacaggat ctccccggat tcccccaaaa agatacccgt
     4141 ggaactggaa gggtgggaaa aaataaaaga gaaaccgata ctcgcctggg ataacgggat
     4201 gcccgcaccg ttgctggtaa gcggcagcaa tgtatggacc ctttcctaca acggggcaaa
     4261 actgcagtcc cggctgatct gtaccgccct gccgcgggac gcccttctcg cttttctcaa
     4321 atacgacagc gccagcgggc tgctttttgt gggaacacaa tcaaggggga tcctcatcat
     4381 ccggaagaac gaggtacgat cggtgagaaa gacccttccg gcccccgagc agccgacggc
     4441 ctattacagc cagctggcgc tccccggagg cgaattcctt tccgggaagg gggatcaatt
     4501 gggcggtggc ccaaacagcc cggcggtgct ggcgatgccg ggaaccttca acaactttat
     4561 ctttaccgac cctgacagca attgctggta tagtcacggg gacacggtct atactttctc
     4621 ttaccggacg caccggtccc gggccttcgc ggcggggtcc ggctccatca ccgacggctt
     4681 tgcctggtcg gaaggccacc tctatatcgc caatgccatc ggtgtcggga tgctgggggg
     4741 ggagggcatc gattaccagt accggtatcc acaggccaac atcaacagca atgtgccctt
     4801 tgcgatggtg gagatcaacc cgggagccat cgcgatcgcc acctgcaacg gcctgttccg
     4861 gtacaatatc gccggtcatc gcctcgacac cctgttacgc atacccggca tctgtgtgcg
     4921 ggccctctgg aaatacaagg gctatctcct tatcggaacg tatggccggg gcatctgggt
     4981 ctaccgcgac ggcgccatcc ggcccatccc cccggacaaa aagggctacc tgctctatgc
     5041 ccattgtttt gttcccgacg gattgggcta ttgctggatc agcaccaacc gcgggctgtt
     5101 ccgggcgcgc ccggacgata tgaccgaggc attcgataag gatctccggg aggtgtatta
     5161 tcactattat ggccgtgagg acgggatgga catgaccgag ctcaacggcg gatgcacccc
     5221 ctgtgcactg cccttgaacg atacgatcct ttcctttccg tcgatggacg ggctggtctg
     5281 ggtaaacccc cagggtccca tccctaccct gcccgaaggc tcgatctata tcgacgcgtt
     5341 catggccgac agtcagcggg tgaatatcac cagcctcgta aagccgctgc ttcccgccag
     5401 tacccgcgat ctccatttcc agctggcctt tcccgcctgg gtcaacaaag aaaatctgaa
     5461 tatcgaatac caattggagc cctggtccaa ggactgggaa aacctcgatc tgacccatgg
     5521 accggaactg catttcagca acctgcccta tggggaatac cggctgctgg tccgcaaggc
     5581 cgatggacc
//
LOCUS       DATGFG010001135         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_754663, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001135 DATGFG010000000
VERSION     DATGFG010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754663"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VKQ52_21540"
     CDS             complement(<1..880)
                     /locus_tag="VKQ52_21540"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ89858.1"
                     /translation="MIPIKTAICSFGMSGRVFHAPFLHVNPRFEFYSVWEREKDLARS
                     IYPDVKVVRTLEDLLADPAVELVIVNTPNYTHFDYAKKALQAGKHVIVEKPFAVTEKE
                     GLELIALAKQVDKKISSYQSRRFDSDYRTVKRIVDSGVLGKLVEAEFHYDRFTPALSP
                     KVHKETPGPGRGLLYDLGSHMIDQALQLFGDPEAVFADIFIIRDNSLVDDYMEVLLFY
                     PGLRCRIKSSYLVREPLPSYSLFGHKGTFLKNRTDTQERHLLAGEMPVGDNWGVEPES
                     EQGLLHTEIDGKIIREH"
     gene            complement(886..>2516)
                     /locus_tag="VKQ52_21545"
     CDS             complement(886..>2516)
                     /locus_tag="VKQ52_21545"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HLZ89859.1"
                     /translation="IALLLIFNSTFAQEYSWSNLPVIHRPTFKKDTFSIKKYGAIADG
                     ITLNTKAINAAIADCSSKGGGVVLIPAGLWLTGPITLRSNVNLHVHRAAILQFTDDKD
                     QYPIVAGNWEGHPAARCQSPISGADLQNIAITGGGIIDGNGDSWRWIAKDRLTEAEWK
                     HKVASGGVVTEDGKTWFPSAKSLKGYRTKDAGWLKPGMSTADFEDIRDFLRPNLLVLT
                     NCKKVLLDGPSFQNSPAWCLHTLLCEDLSLDDVHVRNPWYAANGDGIDVESCKAVLIE
                     NSTFDAGDDGICVKSGRDAEGRKRGRPTEEMIVRNDIVYRAHGGFVVGSEMSGGARNI
                     FVSDCSFIGTDIGLRFKTVRGRGGMVEKIYVKNINMRNIVHQAVFLDMYYFAKPPSLA
                     DVYNGIAKIDLPPVSEATPQFRDIHIHNIICDGAEEGIFVRGLPEMNIRDIDLGDMVL
                     KADKGAEVIEAQNIGLKNITFVTSDSTSPVVYVENSANLHFDTIHYNAGAGLLFSING
                     NKCANIRVADTDRSKAVHDAAFNYGAAKDALEVK"
BASE COUNT          506 a          602 c          797 g          611 t
ORIGIN      
        1 catgttctct gatgatcttt ccatcgatct cggtgtggag caacccttgt tcggattcgg
       61 gttccacgcc ccagttgtcg ccgaccggca tttcgcccgc gaggaggtgt cgctcctggg
      121 tgtcggtgcg gttcttcagg aaggttcctt tatgtccaaa aagactataa gacggcaggg
      181 gttcgcgaac gaggtagctc gatttgatac ggcaccggag gccgggatag aaaaggagga
      241 cttccatgta atcgtcaacc agggaattat cgcgaatgat aaaaatatcg gcgaatactg
      301 cttcggggtc gccgaataat tgcagggcct ggtcgatcat atgggacccg aggtcgtaga
      361 gaagaccgcg accggggccg ggggtttcct tgtggacctt ggggctgagg gccggtgtaa
      421 agcgatcgta gtgaaactcc gcctcgacca gtttaccaag gacgccggag tcgacgatcc
      481 gtttcaccgt gcgatagtca ctgtcgaagc ggcggctttg ataggaggag atctttttat
      541 ccacctgttt tgccagggcg atgagctcaa ggccttcctt ttccgtcacg gcgaagggtt
      601 tttcgacgat gacatgtttg ccggcctgga gggctttttt tgcatagtcg aaatgggtgt
      661 aattgggggt gttgacgatc accagttcga cggcggggtc ggccaggagg tcttcgaggg
      721 tgcggacgac ttttacgtca ggatagatgg accgggcgag gtccttctcg cgttcccata
      781 cggaatagaa ttcgaagcgg gggtttacgt gcaggaaggg cgcgtgaaat acgcgtccgg
      841 acattccgaa agagcaaata gccgtcttga taggtatcat agtgatcatt ttacttctaa
      901 agcgtctttc gcggccccat aattaaaggc tgcgtcgtgg acggcttttg aacgatcggt
      961 atctgctacc cggatgtttg cgcatttatt cccgttgatg ctaaaaagca agccggcacc
     1021 cgcattgtaa tggatagtat caaaatgcag gttggcgctg ttttccacat agaccacagg
     1081 cgatgtcgag tcgctggtga cgaaggttat attcttcagg ccgatgtttt gcgcttcgat
     1141 aacctcggcg cccttatcgg cttttagcac catgtccccg aggtcgatat cccggatgtt
     1201 catttcgggc agtccacgca caaatatacc ttcttcggcc ccgtcacaaa taatattatg
     1261 gatatgaata tcacggaatt ggggggttgc ttcgctaacg ggagggaggt cgattttagc
     1321 aattccgttg tagacgtccg cgagggatgg gggttttgca aagtagtaca tatcgaggaa
     1381 gacggcctgg tgcacgatgt tgcgcatgtt gatgttcttc acatatattt tttcaaccat
     1441 accaccgcgg ccgcggaccg ttttgaagcg aaggccgatg tcggtgccga taaaggaaca
     1501 gtcgctgaca aagatgttgc gggcgccgcc ggacatctcg ctgccgacga cgaagccgcc
     1561 gtgggcgcgg tagacgatat cattgcgaac gatcatttcc tccgtgggac gaccccgttt
     1621 ccgaccctcg gcatcacgtc cggatttgac gcagatgccg tcgtcgccgg cgtcgaaggt
     1681 gctattctct atcaggactg ctttgcagga ttcgacgtcg atgccatcgc cgttggcggc
     1741 ataccagggg ttgcggacat ggacatcgtc gaggctgagg tcctcgcaga ggagggtatg
     1801 gaggcaccag gcgggggagt tctggaagct ggggccgtcg aggaggactt ttttgcagtt
     1861 ggtgagtacg aggagattgg ggcggaggaa gtcgcggata tcctcgaaat cggcggttga
     1921 catccccggt tttagccagc cggcgtcttt tgttctatag ccctttagtg atttggcgga
     1981 tgggaaccag gttttcccgt cttcggtgac gacgccgccg gaggcgacct tatgtttcca
     2041 ttctgcttcg gtgagccggt cttttgcgat ccagcgccag ctgtcgccgt tgccgtcgat
     2101 gataccaccg ccggtgatgg cgatattctg caggtcggcg ccggaaatgg gcgactggca
     2161 gcgggcggcg ggatggcctt cccagttgcc ggcgacgatg ggatactggt ctttatcatc
     2221 ggtaaactgg aggatggcgg cgcgatggac gtgcaggttg acattcgagc gaagggtgat
     2281 gggaccggtg agccagaggc cggccgggat gaggaccact cccccaccct tgctgctaca
     2341 gtccgcgatg gccgcgttga tggcttttgt gttcagggtg atgccgtcgg cgatggcgcc
     2401 gtattttttt atcgaaaagg tgtctttttt gaaggtgggg cgatgaatga cgggcagatt
     2461 ggaccagcta tattcctgcg cgaaggtgga gttgaagatc aacagcaagg caattg
//
LOCUS       DATGFG010001136         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1329074, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001136 DATGFG010000000
VERSION     DATGFG010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329074"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VKQ52_21550"
     CDS             <1..504
                     /locus_tag="VKQ52_21550"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89860.1"
                     /translation="TMFFTNHEDEMDVEDNAVYKFMKRLLPLTGNDGGGKMRVFIDGK
                     RRFTTLFVVVVLLATTDIVFAVDSIPAVFGITQERIVIYTSNIFAVLGLRSLFFLLRG
                     AVDQFSHLQQGIAVVLVFVGVKMLVEYFDIVIPVYVSLLVILVCIAGGILYSIWSNRR
                     NKINDGV"
     gene            494..>2291
                     /locus_tag="VKQ52_21555"
     CDS             494..>2291
                     /locus_tag="VKQ52_21555"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HLZ89861.1"
                     /translation="MGYSIRYIADAVGGRLAPGLPDAGAGPSGAGANENIEHLLLDSR
                     RLIFPATSLFFALKGPRRDGGAFINELCKRGVRNFVLESAADLPGANQVIVKDALAAL
                     QQLAATHRRQFTLPVIGITGSNGKTIIKEWLNQLLEEDYHIVRSPKSYNSQTGVPLSV
                     WQLGPQHQLAIFEAGISRRGEMARLEPVIRPTIGVFTNIGEAHSEGFSSLAEKAHEKL
                     KLFAHAETLVFCSDQPGTVKAVAASIPSHPTNPSGQAGTPGPSVPTLFSWGHNEDATL
                     RIRSIDKRSGWTVIGLAHNGTELSISIPFTDPASIENALHCVAVMLLLGRTAEQIKTR
                     LARLAPIAMRLELKSGINHCSVINDSYSADLNSLSIALDFLSQQQQHNRRTVILSDFL
                     ESGRDENDLYATIARTLGQKQVDRLVGIGPRISIHATAFEQWFPGEAVFYPTVEAFRN
                     DLSRLHFRDETILIKGARIFEFEEIDRLLAEQIHQTVMEVDLNAMAQNLRQFRQLLQP
                     ATRVMAMVKAFGYGSGSFEIANLLQFHGVDWLGVAYADEGVTLRKGGIRVPIMVMNTE
                     NSSFDLLVEYNLQPVIYSFSLLRTFDRWLKKEG"
BASE COUNT          496 a          730 c          627 g          438 t
ORIGIN      
        1 acgatgttct ttaccaacca cgaggacgag atggacgtgg aagataacgc ggtgtacaaa
       61 ttcatgaagc ggctgctgcc gctcaccggt aacgacggtg gcggaaaaat gcgcgtgttc
      121 atcgacggca aacgacggtt caccaccctc ttcgtcgtag tggtgctgct ggcgacgacg
      181 gatatcgtct ttgccgtcga cagcatcccg gccgtattcg gcatcaccca ggaacggatc
      241 gtcatctata catcgaatat ctttgcggtg ctgggactcc gctccctgtt cttcctgctc
      301 cgcggggcgg tggatcagtt cagccacctc cagcagggca tcgccgtcgt gctggtcttt
      361 gtcggggtca agatgctcgt cgaatatttc gatatcgtga tcccggtgta cgtatccctg
      421 ctggtgatcc tggtctgcat agcaggcggc atcctctatt ccatttggtc gaaccggcga
      481 aacaaaataa acgatggggt atagcatccg atatattgcc gatgccgtcg gcggccgcct
      541 ggcgccgggg cttcctgatg ccggtgccgg tccttccggc gcgggggcaa acgaaaacat
      601 cgaacacctg ctgctcgaca gccgccgcct gatctttccc gcaacctccc tgttctttgc
      661 cctcaaaggc ccaagacgcg acgggggggc cttcataaac gaactctgca aacggggcgt
      721 ccggaacttc gtgctggagt cggcggccga tcttcccggc gccaaccagg tcatcgtaaa
      781 ggatgcactg gccgccttgc aacaactcgc tgccacccac cgccggcaat tcaccctccc
      841 cgtcatcggc atcaccggca gcaatggcaa gaccatcatc aaggaatggc tgaaccagct
      901 gctcgaagaa gattaccaca tcgtacgcag ccccaagagc tataattccc aaaccggcgt
      961 accactaagc gtatggcagc tcggaccaca gcaccagctg gccatcttcg aggcggggat
     1021 ctcgcggcgc ggggaaatgg cgcggctcga accggtgatc cggcccacga tcggggtatt
     1081 caccaatatc ggcgaagccc atagcgaagg attctccagt ctcgccgaaa aagcacacga
     1141 aaaattaaag ctgttcgccc acgcggaaac ccttgtcttc tgcagcgacc agcccgggac
     1201 ggtaaaggcc gtcgccgcat ccatcccgtc acacccaacg aacccatccg ggcaagcggg
     1261 gacaccgggg ccatcggtcc caacgctttt ttcctggggc cataacgagg acgcaaccct
     1321 gcgcatccgc tccatcgaca aacgatccgg ctggaccgtc atcggcctcg cacataacgg
     1381 tacggaatta tccatctcca tcccctttac cgaccccgca tcgatcgaga acgcgctcca
     1441 ttgcgtcgcc gtaatgctcc tgctcggccg gacggcggaa cagataaaga cccggctggc
     1501 ccggctggcg ccgatcgcca tgcgcctgga attgaaaagc ggcatcaacc attgttcggt
     1561 gatcaacgac agttacagcg ccgacctgaa ctccctgtcc atcgcactgg acttcctctc
     1621 ccagcagcaa caacacaacc ggcgcaccgt catcctctcc gacttcctcg aaagcggccg
     1681 ggacgagaac gatctctatg ccaccatcgc ccggaccctg ggccagaaac aggtggaccg
     1741 gctggtcggt atcggtccgc ggatcagtat acacgctacg gcctttgaac aatggttccc
     1801 gggcgaagcc gtgttctatc ccacggtcga agcctttcgc aacgacctgt cgcggctgca
     1861 tttccgcgac gagacgatcc tgataaaagg agcacgcatc ttcgagttcg aagagatcga
     1921 ccggctcctg gcggaacaga tccaccaaac cgtcatggag gtggacctca acgcgatggc
     1981 gcagaacctg cggcagtttc gccagctgct ccaacccgcc acccgcgtga tggcgatggt
     2041 aaaggccttt ggctatggaa gcggcagttt cgagatcgcc aatctcctcc agttccatgg
     2101 cgtcgactgg ctcggcgtgg cctatgccga cgaaggggtg acgctgcgaa aaggcggtat
     2161 tcgggtgccc atcatggtga tgaacacgga gaacagcagt ttcgacctgc tggtggaata
     2221 caatttgcaa cccgtgatct attccttttc actgttgcgc accttcgacc gctggctcaa
     2281 aaaggaaggt a
//
LOCUS       DATGFG010001137         7042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1324404, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001137 DATGFG010000000
VERSION     DATGFG010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324404"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VKQ52_21560"
     CDS             <1..808
                     /locus_tag="VKQ52_21560"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HLZ89862.1"
                     /translation="IAVAEAARNIVCSGGQPLGVTNCLNFGNPYDPEVYYQFVHAIKG
                     MGDACRKFDTPVTGGNVSFYNQNPDGAVYPTPTIGMVGLLDSVRSKMTLDLKEEGDVL
                     FLMGESYDDINSSEYLHKVKKVEFSPAPHFDIEEEYRLQQKVAELIGKKLVRSAHDVS
                     EGGLFVTLAESAFPRGLGFDVVTTDAAIRKDAYWFGEAQSRVVVSVSASRVEEFRKAL
                     GGFPAAKLGVVTGGGFVVDGKDWGRVEEWKEKYDHSLENYMARLVDIDQL"
     gene            811..1821
                     /gene="rfaD"
                     /locus_tag="VKQ52_21565"
     CDS             811..1821
                     /gene="rfaD"
                     /locus_tag="VKQ52_21565"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HLZ89863.1"
                     /translation="MVDANKEYIVVTGAAGFIGSCLVGFLNQHGYTDLILVDEFGDPE
                     KEPNLAGKSFAEKVEREGFFDWLETRRPRLSFVFHIGARTDTTEFDYSIHEHLNVEYS
                     QKMWVYCTEHGIPLVYASSAATYGGGEKGYDDRHELAFELKPLNPYGVSKNEFDKWAL
                     QRAALDGGSGHEAPPFWAGLKFFNVYGPNEYHKARMASVIFHSFNQIKKDGFVKLFRS
                     HRPDFKDGQQLRDFVYVKDVIKVCYWLMEHRPASGLYNLGTGKARSFEDLVKATFAGL
                     DLPAKIEYIDMPEDIRDKYQYFTEANMGKLRAVGYKNAFHTLEEGVVDYVRNYLAKKH
                     YY"
     gene            1870..2667
                     /gene="proC"
                     /locus_tag="VKQ52_21570"
     CDS             1870..2667
                     /gene="proC"
                     /locus_tag="VKQ52_21570"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HLZ89864.1"
                     /translation="MNKKIAIIGGGNLGTAIAEGLIQSGFVAPKHILITKRNIHTLHD
                     LERKGVLVSDKNEEAVRYADLVILAVKPFQVNDILLSLKGEFRAEKQVLVSVVTGISI
                     EHIVGYLGNGVPVVRAMPNTAIAIQQSMTCIATRNVSEEHFQYVMDIFNQLGKAVRID
                     EKLMDAATVLGACGTAFAMRYIRANIQGGIEIGFDAATANLIAAQTVKGAAELLLQKG
                     THPEQEIDKVTTPKGCTIAGLNEMEHRGFSSSLIKGLVASYDKIAKD"
     gene            complement(2740..3669)
                     /locus_tag="VKQ52_21575"
     CDS             complement(2740..3669)
                     /locus_tag="VKQ52_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89865.1"
                     /translation="MAQQSPSLSALPSVIITLRDPGPVATGVFSRFEVLDERSDTSRI
                     GVHTFLPTIGHPRDRQLVFPSPAATAIAGYLNTHFTRPDAPYTALIVLRNLWLSDASY
                     LREELKKDPNKLYVRTHIRFKAEIYAVRDSQYMPILRFDTLQAYKRSNMYSSTSSFYD
                     LWDRDLTSILNEMADSASQLTTAKAGHTRLLHLDDIVEFNRSRFSAPISANTSLTPGV
                     YNSFEEFRNNTPSIRDFEIKTENNARVLYIKETGSSAWYYSHDAWGYCDGKSVFIMRN
                     GVLHRAWKEGKAFYFIGGPDKELAIDMDTGLVY"
     gene            complement(3790..4179)
                     /locus_tag="VKQ52_21580"
     CDS             complement(3790..4179)
                     /locus_tag="VKQ52_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89866.1"
                     /translation="MARETGAFGRVSVTSEGIRREISFVAVVSDLVIFTACRTEIETI
                     SITNTSPVRVFPFMVKLDLGLFYGIEINTLILVFRERNLRPLSLKRKIIPLYFPRKVE
                     IGQFLSDFYMSFFHFATLYNHFTIIYI"
     gene            4471..6129
                     /locus_tag="VKQ52_21585"
     CDS             4471..6129
                     /locus_tag="VKQ52_21585"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HLZ89867.1"
                     /translation="MAKYVFVTGGVTSSLGKGIIAASLAKLLQARGLRVTIQKFDPYI
                     NVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNIFTSQANNVTTGRIYQTVINKE
                     REGAYLGKTVQVIPHITDEIKRRMLLLGQGNKWDVIITELGGTVGDIESLPFIEAVRQ
                     LQWEMPEEDCVVVHLTLVPYLKAAKELKTKPTQHSVKLLSQEGVHPDIIVCRTEEPLS
                     PDIRKKIALFCNVKPEAVIEAADAPTIYEVPLTMMREKLDLICMKKLNITHYHEPELS
                     RWKEFLDKLKYPKSKVTIGLIGKYIELQDAYKSILESFVHAGAMNECKVQVVNVHSEF
                     ITPENVAEKLANLDGLLVAPGFGHRGVEGKIIAVRYARENKLPFFGICLGMQMAVIEY
                     ARNVLGLKDAHSTEMDVHTPHPVIDLMEEQKKVTAKGGTMRLGSYPCSLKEGSLASQV
                     YDGKLEIAERHRHRWEFNNAYLQQFEQAGLIPSGINPESGLIEIVELSANTHPFFFGV
                     QYHPELKSTVENPQPIFVHFIKAAKEFAEAKNAVKNPLLQSEMI"
     gene            6334..>7042
                     /gene="yidC"
                     /locus_tag="VKQ52_21590"
     CDS             6334..>7042
                     /gene="yidC"
                     /locus_tag="VKQ52_21590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HLZ89868.1"
                     /translation="MDRNTVIGFVLLGLMLFLYLYISTKNSHELEARTKHIADSVALV
                     KQVREAADAKEAATHQDTAAQQPVDTTGFAMAFKGMEQQLTVENDLIKVVFSNKGGQP
                     VAVELKKFKAYDSTAVKLVGPDNRISYPINTGAGAADIAQLYFRPGQVVRNADGSQTV
                     RFRLPTPAGESIEHEYTLAANDYTLDWNVNINNPEKLFSQGNFNLGWHIETQRAQKNI
                     SDERRLTSIGYYEDNRFD"
BASE COUNT         1627 a         1510 c         2236 g         1669 t
ORIGIN      
        1 gatcgcggtg gcggaagccg cgcggaatat cgtgtgcagt ggcggccagc cgctgggggt
       61 gacgaactgt ctcaattttg gcaatcctta tgatccggag gtgtattacc agtttgtcca
      121 tgcgatcaag gggatggggg atgcgtgccg gaagttcgat acgccggtta ccggcggaaa
      181 tgtcagcttt tacaaccaga accctgacgg cgccgtctat ccgacgccca ccatcgggat
      241 ggttgggttg ctggacagtg taaggagcaa gatgacgctg gacctgaagg aagaggggga
      301 tgtacttttc ctgatggggg aaagctatga cgatatcaat tcttcggagt acctgcataa
      361 ggtgaaaaag gtggagttca gtcccgcgcc gcatttcgat atcgaggagg aataccggtt
      421 gcagcagaag gtggcggagc tgatcgggaa gaagctggtc cgcagtgcgc atgatgtgag
      481 cgagggcggt ctttttgtga cccttgccga gtcggctttc ccgcgtgggc tcggattcga
      541 tgtcgttacc acggatgccg cgatccgcaa ggatgcgtac tggtttgggg aagcccagag
      601 cagggtagtg gtgagtgttt cggcatcgcg ggtggaggaa ttcaggaagg cgcttggcgg
      661 ttttcccgcg gcgaagctgg gcgtcgttac cggcggcggt tttgtcgtcg atgggaagga
      721 ttggggaagg gtagaagagt ggaaggaaaa atatgaccac agcctggaga attatatggc
      781 aagattggta gacatagatc agttataagt atggttgacg ctaataagga atatatcgtt
      841 gttaccggtg cggccgggtt tatcgggagt tgtctcgtcg gttttttgaa ccagcatgga
      901 tatacggatc tgatcctcgt ggacgagttt ggcgatccgg agaaggagcc caatcttgcg
      961 gggaagtcgt ttgcagaaaa ggttgagcgg gagggattct tcgactggct ggagacccgg
     1021 cggccgaggc tttcttttgt ctttcatatc ggtgcgcgga cggatacgac ggaattcgac
     1081 tattccattc atgagcatct gaatgtggag tattcccaga agatgtgggt gtattgcacg
     1141 gagcatggca ttccgctggt gtatgcttct tcggcggcta cgtatggcgg cggggagaag
     1201 ggatatgatg accggcatga gctggctttt gaactgaagc cgctgaatcc gtatggcgtt
     1261 tccaagaatg agttcgataa atgggcgttg cagcgggcgg cgcttgacgg cgggagcggg
     1321 cacgaggctc cgcctttttg ggccgggttg aagttcttca atgtctatgg tcctaacgag
     1381 tatcataagg cgcggatggc cagtgtgatc ttccattctt tcaaccagat caagaaggat
     1441 ggttttgtaa agctgttccg gagtcaccgg ccggatttta aggacggtca gcagttgcgg
     1501 gactttgtgt atgttaagga tgtgatcaag gtgtgttatt ggctgatgga gcaccggccc
     1561 gcatcggggt tgtataacct gggaacgggg aaggcgaggt cgttcgagga cctggtgaag
     1621 gcgacatttg ccgggcttga cctgccggcg aagatcgagt atatcgacat gccggaggat
     1681 atccgggaca agtaccagta ttttaccgag gcgaatatgg ggaagctgcg ggccgtgggg
     1741 tataagaatg cgtttcatac cctggaggag ggtgtggtcg actatgtaag gaattatctg
     1801 gcgaagaaac attactatta ggccgacgat gggagcttgt cggcaggaca gacattttaa
     1861 ctgatgttta tgaacaagaa gatcgcgatc atcggcggtg ggaatttagg aacggccatt
     1921 gccgagggat tgatccagag cggatttgtt gcgcccaagc atattctcat taccaagcgg
     1981 aatatccata cgcttcatga cctggagcgg aagggggtcc tggtcagtga taagaacgag
     2041 gaagctgtgc ggtatgccga cctggtgatc ctggcggtaa agcccttcca ggtgaatgat
     2101 atcctgcttt cgctgaaggg cgagttccgg gcggaaaagc aggtgctggt gtccgtggtc
     2161 accggtatca gtatcgagca tattgtgggt tatctcggga atggggtgcc cgtggtgcgg
     2221 gccatgccca atacggcgat cgccattcag caaagcatga cctgtatcgc cacgcgcaat
     2281 gtgagtgaag agcatttcca gtatgtaatg gatattttta atcaattggg taaggcggtg
     2341 cggatcgatg agaagctgat ggatgccgcc accgttcttg gcgcctgtgg taccgcgttt
     2401 gccatgcggt atatccgggc gaatatccag ggaggtatcg agatcgggtt cgatgcggcc
     2461 acggccaacc tgatcgcggc ccagacggtg aagggggcgg cggagctgtt gttgcagaag
     2521 gggacccatc ccgagcagga gatcgataag gtcactacgc ccaaggggtg tacgatcgcg
     2581 gggttgaatg agatggagca ccggggattc agctcgtcgc tgatcaaggg gctggtggcc
     2641 tcttatgata agatcgcgaa agattgatgc cgtgcggcgc cgggtgatgg ttggtggatg
     2701 atgctggcgg tgcggcagtt gggtgcggca gcggtgtggt taatagacca ggccggtgtc
     2761 catatcgatg gcgagttctt tgtcggggcc gccaataaag taaaaggctt ttccttcctt
     2821 ccaggcccgg tggaggacgc cattgcgcat tataaagacg gacttcccgt cgcagtatcc
     2881 ccaggcatcg tgtgaatagt accaggcaga gctgcctgtt tcctttatat acagcacccg
     2941 ggcattgttc tccgtcttta tttcaaaatc gcggatggag ggggtgttgt tccggaattc
     3001 ttcaaagctg ttatagacac ccggtgtcag ggaggtgttc gcgctgatgg gggcggagaa
     3061 ccgtgagcgg ttgaattcga cgatgtcgtc gaggtgcagg agacgggtgt gcccggcctt
     3121 cgcggtggtg agttgcgagg cgctgtccgc catttcgttc aggatggagg tgaggtcccg
     3181 gtcccagaga tcataaaatg aactggtgga ggagtacata ttactgcgtt tgtaggcctg
     3241 gagggtgtcg aaccggagga tgggcatgta ctggctatcc ctgacggcat agatctcggc
     3301 tttgaaacgg atatgggtcc ttacatagag tttgttggga tctttcttca gttcttcccg
     3361 gagatagctg gcgtcggaga gccagaggtt gcgcaggacg atcagggcgg tatagggggc
     3421 atccggccgg gtgaaatgag tgttaaggta gccagcgatg gccgtggctg cgggggaagg
     3481 gaagacgagc tgcctgtcgc ggggatggcc gatggtcggc aggaaggtat ggacgccgat
     3541 gcggctggta tcggatcgtt cgtcgaggac ctcgaaccgg ctgaagacgc ctgtcgcaac
     3601 ggggccggga tctctgaggg ttataatgac ggagggaaga gcggagagcg atggcgattg
     3661 ttgggccatt ccactgatgg ttaggcacag gcataaaggg cccatgatag atattgggta
     3721 tttcataacg ggtgacgaat catctacaaa atagcaaaaa atccggcttt tgttaaatac
     3781 tattttattt tatatgtaaa tgattgtgaa atgattataa agggtcgcaa aatggaaaaa
     3841 ggacatgtaa aaatccgata aaaactgccc gatttctacc ttgcggggaa aataaagggg
     3901 tattatcttg cgtttgagtg acagcgggcg taaattgcgc tcgcgaaaga caagaatcaa
     3961 agtgttaatc tctatcccgt agaagaggcc taaatccaat ttaaccatga aaggaaaaac
     4021 ccttaccgga ctcgtattgg taatcgagat cgtttctatt tccgtcctgc atgcagtaaa
     4081 gatcactaaa tcggaaacaa cagctacaaa ggaaatctcc cggcggatgc cttcggaggt
     4141 gacagaaacc cggccgaaag cgcctgtttc ccttgccatt ttgaaataaa atcgggtata
     4201 taaagagaag gggaccaacg atagttgatc ccctttttta ttaagttatc cacacgggtt
     4261 tttgcgggaa tataatttcc tgtaactttg catgacctcc ttgaatttcc cttcttatat
     4321 ggttttcagc aggtcatcag cggccaggat tcattcattt tattcatcga gtttccagct
     4381 gagcaggatt gtttgatgct aaggtattgt tttccgcctg tcgcccaaca acacaacaat
     4441 gcatttaata ataaattaat tgaccatact atggctaagt atgtttttgt tacaggtggt
     4501 gtgacttctt cgctgggcaa aggaattatt gcggcgtctc tggctaaact gttgcaggcc
     4561 aggggtctaa gggttaccat tcagaagttt gacccctata tcaacgtgga ccccggcacg
     4621 ctgaacccct atgagcatgg ggaatgttat gtaacggagg atggggcgga gaccgacctg
     4681 gatcttggcc attacgagcg ttttttgaac atttttacct cgcaggcgaa taatgtcact
     4741 acggggagga tctaccagac ggtgatcaac aaagagcggg aaggggccta cctgggtaag
     4801 acggtgcagg tgatcccgca tatcaccgat gagatcaagc ggcggatgtt gctgcttggg
     4861 caggggaaca agtgggatgt catcatcacc gagttgggtg gtacggtggg tgatatcgag
     4921 tcgttaccct ttatcgaggc cgtgcggcag ttacaatggg agatgcccga ggaggattgt
     4981 gtggtggtgc atcttacgct ggtgccctat ctgaaagcgg ccaaggagct gaaaaccaaa
     5041 cctacgcagc atagcgtgaa attgcttagc caggagggtg ttcatccgga catcatcgtg
     5101 tgccggacgg aggagccgtt atcgccggat atccggaaga agatcgcgct tttctgtaat
     5161 gtgaagcccg aggcggtgat cgaggcggcc gatgcgccca cgatctacga ggttccgttg
     5221 acgatgatgc gggagaagct ggatctcatc tgcatgaaga agttgaatat cacccattat
     5281 catgagcctg agctttcccg ctggaaggag ttcctggaca aattgaaata tcccaagagc
     5341 aaggtgacga tcgggcttat cggtaaatat atcgagttac aggatgcgta caagtcgatc
     5401 ctggagtctt ttgtccatgc cggtgcgatg aacgagtgca aggtgcaggt ggtgaatgtg
     5461 cacagtgaat tcattactcc cgagaatgtg gccgagaagc tggccaacct ggacgggctg
     5521 ctggtagcgc cggggtttgg tcatcgtggg gttgaaggga agatcattgc cgtccggtat
     5581 gcgcgggaga acaaattgcc attttttggg atctgtctcg ggatgcagat ggcggtgatc
     5641 gagtatgcgc gtaatgtgct tgggctgaag gatgcgcatt ccacggagat ggatgtgcat
     5701 acccctcatc ctgtgatcga cctgatggag gagcaaaaga aggtgaccgc caagggtgga
     5761 accatgcggt tggggtcgta cccctgttca cttaaagagg ggtcgcttgc cagccaggtc
     5821 tatgacggca agctggagat cgccgaacgg catcgtcatc gttgggaatt caataatgcc
     5881 tatctacaac aattcgagca ggccgggctg atcccgagcg gcatcaaccc ggagagcggg
     5941 ctgatcgaga tcgtcgaatt gtctgccaat acccatcctt ttttcttcgg ggttcagtat
     6001 catccggagc tgaagagcac ggtggagaat ccccagccga tctttgtgca ttttatcaag
     6061 gctgccaagg agtttgcgga agcgaagaat gcggtgaaga atcccctgct gcagagtgag
     6121 atgatctagg gtttaccgac gtctttggcg tccggcgggc cccgggaggg gttccggcgg
     6181 ataagactgt ggtctgtgat tgaaacgcca tatatttgca cttcctaaag ccgccctggc
     6241 ggcttgtccg ttaggacaga cattagcgct ttgcagggga cgcgctaatt ttctgatagt
     6301 gcatcccctg caaattaaaa aactatcatg ggaatggatc gcaatacggt tattggcttt
     6361 gtgttgttgg ggcttatgct atttctttac ctctatatct ctaccaagaa cagtcatgag
     6421 ctggaagcgc ggacgaagca tatcgcggac agcgtcgccc tggtgaagca ggtgcgtgag
     6481 gctgccgacg caaaagaggc tgctacccat caggataccg cggctcagca gccggtggat
     6541 acgacggggt tcgccatggc cttcaagggt atggaacaac agcttaccgt ggagaacgac
     6601 ctgataaagg tggtttttag caataagggc gggcagcctg tagccgtcga gctgaagaaa
     6661 ttcaaggcct atgacagtac ggcggtgaag ctggtgggac ccgataaccg gatcagctat
     6721 ccgatcaata cgggggcggg cgcggccgat attgcccaat tgtatttccg gcccgggcag
     6781 gtcgtcagga atgcggatgg cagccagacg gttcgtttcc ggcttccgac gcctgcgggt
     6841 gagtccatcg aacatgaata tacgctggcg gcgaatgatt atacgcttga ctggaatgtc
     6901 aatatcaaca acccggagaa gctgttcagc cagggtaatt tcaacctggg ctggcatatc
     6961 gagacgcaac gggcccagaa gaatatctcc gatgagcgcc ggttgaccag catcggttat
     7021 tatgaggata accggtttga ct
//
LOCUS       DATGFG010001138         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1211848, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001138 DATGFG010000000
VERSION     DATGFG010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211848"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VKQ52_21595"
     CDS             <1..920
                     /locus_tag="VKQ52_21595"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ89869.1"
                     /translation="ASFTMFVQPLPKSFNSNNEITPPVYSDHVLFFEQLQRPYECPEH
                     PANIGLLIAAKGSCHYYVNGARNQVEDNKVFFISRGSRLAIRNAGKDTAPALLFFHSR
                     LPDLVQHSLNYGGEVLLEKPFDSLPYDFSWLERIHVDPELYQTVFSLIDLGSSCSSFA
                     SLQADMIIRQLFERLLIKNQDAYRRSQNVQAVKASTRLEIFKRISTARDWMDAHYNTE
                     ISLEDIGAVAAMNSQHFLRMFKQVYAITPHQYLIDLKLKKAKELLESTELTINEICQV
                     IGFESLFSFSILFKGRFGLPPSQFRKTGT"
     gene            1070..1579
                     /locus_tag="VKQ52_21600"
     CDS             1070..1579
                     /locus_tag="VKQ52_21600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLZ89870.1"
                     /translation="MTQLPKADFSQFKLRVNIKTNVENAYRAWASPSGLASWFLGKVT
                     FTDASGAARTDDSLAQEGDEYVCYSNRRPDTVIIKGKVLKANGRNLYSFSFSKECPVT
                     ISMYMEHDETIVELIESNLPTDEETMRRHYVGDSKGWIFYLTNLKSVLEGGLDLRNKN
                     ADIVNVITH"
     gene            1607..2041
                     /locus_tag="VKQ52_21605"
     CDS             1607..2041
                     /locus_tag="VKQ52_21605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89871.1"
                     /translation="MKQQDYRCSIAADITPEEAMECISDVQAWWAKHFEGSSRLLHDV
                     FTVRFGTTYVTFQITELIPEQRVVWYVTDCYLPFISDKHEWTGTSILWEIEAAGDETR
                     IDMTHLGLIPEAECFDNCQRGWNRHIKESLLRYITEQVGMPS"
     gene            complement(2116..>2425)
                     /locus_tag="VKQ52_21610"
     CDS             complement(2116..>2425)
                     /locus_tag="VKQ52_21610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLZ89872.1"
                     /translation="WSALEALGRSLSVEYGQYGVRSVCLLTTAIPETPLIDEVFNIHG
                     KAHGTDYDQFRSFMEGMTHRKRLTSLGELAGAAVFAASGEGGAVTGTILNLTSGMIVN
                     "
BASE COUNT          598 a          679 c          618 g          530 t
ORIGIN      
        1 tagcaagttt tacaatgttc gttcaaccac tgccgaaatc cttcaacagt aacaatgaaa
       61 taaccccgcc ggtttattcc gatcatgtcc tgttcttcga acagcttcag cgcccatatg
      121 aatgcccgga gcatcccgcc aatatcggcc tgctgatagc ggcaaaaggg agttgccatt
      181 attatgtcaa cggcgccagg aaccaggtgg aagacaacaa ggtattcttt atctcccggg
      241 ggagccggct ggccatccgc aacgccggga aggatacggc tcccgcactc ctgttctttc
      301 attcgagatt acccgacctg gtacagcaca gcctcaatta tggcggcgag gtactcctgg
      361 aaaaaccctt cgatagtctg ccctatgatt tttcctggct cgaacgcatc catgtcgacc
      421 ctgagctata ccagaccgtt ttctcgctga tcgacctggg cagcagttgc agttcgttcg
      481 cctccctgca ggcggatatg atcatacgac aattgttcga gcgactgctg atcaagaacc
      541 aggatgccta taggcggtcg caaaatgtgc aggccgtaaa ggcctcgacc cggctcgaga
      601 tcttcaaacg catctcgacg gcgagggatt ggatggacgc ccattataac accgagatca
      661 gcctggagga tatcggcgcc gtcgccgcga tgaacagcca gcattttctc cggatgttca
      721 agcaggtgta tgctatcact ccccatcaat acctgatcga tttgaaattg aagaaggcaa
      781 aagagttgct ggaaagtacc gaattgacga tcaatgagat atgtcaggtc atcggttttg
      841 agagcctgtt ttccttcagt atcctcttca agggccgttt tggcctgccg ccctcccagt
      901 tccgcaagac cgggacctga gccgtctccc gcaagacggg agcttgagcc atcttttcgc
      961 aagaccgggg ctggtccgcc ggaacgcgtc ctgaaattgt ccattttcga taaacccttt
     1021 cgtcgccggg aggattagct ttgtcttatc aacaaaaaat aaaaagacaa tgacacaact
     1081 tccaaaggcg gatttcagcc agttcaaact gcgggtaaac atcaaaacaa atgtcgaaaa
     1141 tgcctaccgg gcctgggcaa gtccgtcggg cctcgcttcc tggttcctgg gaaaagtgac
     1201 ctttaccgat gcttccggcg ctgcgcgcac tgatgactcc cttgcgcagg agggcgatga
     1261 atacgtctgt tattcaaacc gccggcccga taccgtgatc ataaaaggga aggtgttaaa
     1321 agcaaatggc cggaacctct atagcttttc tttttccaaa gaatgccccg tcaccatctc
     1381 catgtatatg gaacacgacg agaccattgt cgaactgatc gaaagcaacc tgccgacgga
     1441 tgaagagacc atgcggcggc attatgtcgg cgattcgaaa ggctggatat tctacctgac
     1501 caatcttaaa tccgtccttg aaggcgggct cgacctgcgg aacaagaacg cggatatcgt
     1561 caacgtcatc acccattgat tttctaacaa ataaaaataa aacaaaatga aacagcagga
     1621 ttatcggtgc agcatcgctg ccgacatcac ccccgaagaa gccatggaat gcatcagcga
     1681 tgtccaggcc tggtgggcca aacatttcga agggagctcg cgcctattgc atgacgtttt
     1741 tacggtgcgt tttggcacca cctatgttac ctttcagatc accgaactca tccctgagca
     1801 gagggtagta tggtatgtca cggattgcta cctgcccttt atcagtgaca aacacgaatg
     1861 gacgggcacc agcatcctct gggagatcga agcggcaggc gatgagaccc gcatcgacat
     1921 gacgcatctc ggtcttatcc cggaagcgga atgttttgac aattgccagc ggggatggaa
     1981 ccggcatata aaggaaagcc tgctccggta tataacggag caggtgggga tgccgtcctg
     2041 aggaccccgc agcgccgtcc gggcggccgc gccctccgtt cgggggcgcg gccgcgttcc
     2101 ttagttcacc atcatctaat tgacgatcat ccccgaagta agattcagga tcgttcccgt
     2161 aacggcgccc ccttccccgg aagcggcgaa tacggctgca cccgccagct ccccgagcga
     2221 tgtcaggcgt tttcggtgcg tcataccttc cataaaggaa cggaactggt cataatcggt
     2281 gccatgcgcc ttgccatgga tattgaacac ctcgtcgatg agcggggtct ccggtatggc
     2341 cgtcgtcaat aagcacaccg aacgcacccc atactggcca tattccacgg acaatgaacg
     2401 gccgagcgcc tcgagagccg accag
//
LOCUS       DATGFG010001139         5399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_985302, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001139 DATGFG010000000
VERSION     DATGFG010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985302"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VKQ52_21615"
     CDS             <1..1441
                     /locus_tag="VKQ52_21615"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HLZ89873.1"
                     /translation="AAKVLLATVKGDVHDIGKNIVGVVLGCNGYDIIDLGVMVPADKI
                     LETAKREKVAIIGLSGLITPSLDEMVHVAREMKRQDFEVPLLIGGATTSRMHTAVKIA
                     PEFDKGVVHVLDASRSVTVVGSLLSAEQKPDFLRTIYSEYRKLKEDFTNKKSVKQYLS
                     FGQAQENKVPIDWNGFRPVAPGFVGTKAFDGYDLAEIVPYIDWGPFFIAWELHGKFPQ
                     ILQDEKVGTEATRLYNDARQLLDQIVREKWLKPRAVIGFWPANSDGKDTVVVKTGGGE
                     VRLECLRQQIKKAPGQPNISLADFIAPAETGKTDHIGGFVVSIEGIEPYIKRFEAAHD
                     DYNKITLQALADRLAEAFTELLHEKVRKEYWGYAKEEHLGNEQLIGEEYLGIRPAPGY
                     PACPDHTEKIKLFAMLDATKQTGTILTESLAMYPASSVSGWYFAHPQSKYFGVGKIER
                     DQVTEYAARKGMPLAEVEKWLRPVLEYDI"
     gene            complement(1444..1866)
                     /locus_tag="VKQ52_21620"
     CDS             complement(1444..1866)
                     /locus_tag="VKQ52_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89874.1"
                     /translation="MEDTALLTKQIKTAQILLFIVAAVTLISAILLLPDAFEREKAGG
                     PLLALALAAVYLALGIYAKKKPYTALRAGLGVILLIILVNLIINPSGIFGHWASRVLS
                     ILLLCLGLADGRDAERKMTGPPAKEQAKEQAPAPHTNA"
     gene            1980..2810
                     /locus_tag="VKQ52_21625"
     CDS             1980..2810
                     /locus_tag="VKQ52_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89875.1"
                     /translation="MMSSGARYRNLTLSLFTIALPFFAFSQQQPEKKKKKLIKEVYVS
                     WGYNGEWYTHSTIHISQPSLGNNYDFVHVKAHDHPGWNEGLFSKALSIPQYNYRLGFL
                     LDEEKGWGFEINFDHTKYIFADQDVRLKGTLGKRPIDTTIAFNQANGFYYYLNNGANF
                     LLFNVTKKWHLLKTDDGKVKVDFLGKFGVGPVIPHTQNSLFGHPNHEGFQFGGWNTGL
                     EGSLRATFFRYAYLEYSNKIDYARYSGLNIYEGKAHQAFGTYEMILSIGFVIPTTAKK
                     "
     gene            complement(2785..3552)
                     /locus_tag="VKQ52_21630"
     CDS             complement(2785..3552)
                     /locus_tag="VKQ52_21630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLZ89876.1"
                     /translation="MEQLNRVIPAKDVQFLDGPKSRPYEFSFAWRVFREFIKGFRTLH
                     FVGPCITVFGSARFKEDHPHYQSAREMGRQIATLGLTTMTGGGPGIMEAANRGAFEQG
                     GMSVGCNIKLPFEQAPNPYVQTSITFDHFFVRKVMLVKYSYAFVIMPGGFGTMDEFFE
                     TLTLVQTKTLVRFPIVLFGKEYYAPLFEYMESMAGQGTISKEDMSLVLLTDSVAEAIR
                     HIDTFIKKNYREGVRKRKWWLFEKKPATPVTSSQSLG"
     gene            3603..4382
                     /locus_tag="VKQ52_21635"
     CDS             3603..4382
                     /locus_tag="VKQ52_21635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HLZ89877.1"
                     /translation="MSSLRIVSYNVNGIRAAMNKGLVDWLKTDVADVFCVQETKAHRD
                     NVAHALFTDLGFHDYWFPAQKKGYSGVAVFTKVKPDNVVYGTGHAVSDEEGRVIQLDL
                     GDIRLINAYFPSGTSGEERQVYKYQWLDEMHAYLEELKKTHPRLVLCGDYNIAHREID
                     IHDPKGNKNNSGFLPEEKAWMDKFMGSGWIDSFRHFCADPHHYTWWSQRFPSVRLENK
                     GWRIDYISVTAPLKQRVLSAAIYPDVKHSDHCPVYLELAAK"
     gene            4464..>5399
                     /locus_tag="VKQ52_21640"
     CDS             4464..>5399
                     /locus_tag="VKQ52_21640"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HLZ89878.1"
                     /translation="MDIIAILLLCLLIILVLLLRNSLFDRLDRLEREVSRLREEKAAA
                     PPPEAVSVPVAKPPAAVPPSEVPPIAKPRETEPFRPMERPRPVEAPSPRPAAASTPRP
                     AERAAYAPIEPPHFQRMDEVKPPPAAGPEPGFFERHPDLEKFIGENLISKIGIAILVL
                     AIGFFVKYAIDNDWVGPVGRVGIGLLCGGILIGIAHWMRNQYKAFSSVLVGGGLAVLY
                     FTIALAYHQYQLFNQPTAFVIMLVVTAFSVLLSLLYDRQELAIIALVGGFITPFLVSN
                     GRGDYVVLFGYLLILNTGLLVIAYNKAWRLLNILSF"
BASE COUNT         1224 a         1336 c         1579 g         1260 t
ORIGIN      
        1 tgcagccaaa gtattgctgg ctaccgtgaa gggcgatgtg catgatatcg ggaagaacat
       61 cgtgggtgtc gtattgggtt gcaacggcta tgatatcatc gatcttgggg tgatggtgcc
      121 tgccgataag atactggaaa cagccaaaag agaaaaagtg gccatcatcg ggcttagcgg
      181 cctgatcaca ccctctctcg atgagatggt gcatgtagca agggaaatga agcggcagga
      241 cttcgaggtg ccgttgctta tcggcggggc gacgacgtcc cggatgcaca cggcagtgaa
      301 gatcgcgccc gaattcgaca agggggtggt gcatgtgctg gatgcttcgc ggagcgtgac
      361 ggttgtaggc tcgctgctga gcgccgagca aaagccggat ttcctgcgaa ccatttactc
      421 ggaataccgg aagctaaaag aagattttac caataaaaag agcgtcaagc aatacctgtc
      481 cttcgggcag gcgcaggaga acaaggttcc gatcgattgg aacggcttcc ggcctgtggc
      541 cccgggcttt gtcgggacaa aggcttttga tggatatgat ctcgccgaga tcgtgccgta
      601 tatcgattgg ggtcccttct ttatcgcctg ggagctgcac gggaaattcc cgcagatcct
      661 gcaggatgag aaggtgggca cggaggcgac gcggctctat aacgatgccc ggcagctgct
      721 ggaccagatc gttcgcgaga aatggctgaa gccgcgggcc gtcatcgggt tctggccggc
      781 caattcggat ggaaaggata cggtggtggt gaagacgggc gggggtgagg tacgattgga
      841 atgcctgcgg cagcagatca agaaggcgcc cggacaaccc aatatctcgc ttgccgactt
      901 tatcgcgccc gccgaaacag gcaagaccga tcatatcggt ggttttgtcg tcagcatcga
      961 aggcatcgaa ccgtatatca agcgcttcga ggccgcgcat gacgactata acaagatcac
     1021 gttacaggcg ctggcggacc ggttggcgga agcctttacc gagctgctgc atgaaaaggt
     1081 gcgtaaggaa tactggggct atgcgaagga agaacatctc ggcaatgagc aattgatcgg
     1141 ggaagaatac ctggggatcc ggcccgcgcc gggttatccc gcctgccccg atcatacgga
     1201 aaagataaaa ctgttcgcga tgctggatgc gacgaagcag accggcacta ttctgaccga
     1261 gtccctggcg atgtatcccg cgtcttcggt cagcgggtgg tattttgccc atccgcagag
     1321 caagtacttc ggggttggca agatcgaaag ggaccaggtg acggagtatg ccgcccgtaa
     1381 ggggatgccg cttgccgagg tggagaagtg gctgaggccg gtgttggaat acgatatata
     1441 gggctatgcg ttggtatggg gggcgggggc ttgttctttg gcttgctctt ttgccggcgg
     1501 accggtcatc tttctttccg cgtccctgcc gtcggcgagc ccaaggcaaa gcaggaggat
     1561 ggaaagcact ctcgatgccc agtgcccgaa gatgcctgaa gggttgatga tcaggtttac
     1621 caggataatc aggaggatca cgccaaggcc cgcgcgcagg gccgtatatg gttttttctt
     1681 tgcataaatc ccaagggcca ggtatacggc cgccagggca agggcaagga gggggccacc
     1741 cgctttttcg cgctcaaagg catcgggcaa gagcagaatg gcggaaatga gcgtcacggc
     1801 cgcaacgatg aaaagcagga tctgcgctgt ttttatctgt ttggtaagca gggcggtatc
     1861 ttccatgata gtaaagactg atatgtttag cggatttagc ggcgataagg taataataaa
     1921 attactaaat ttgcaccggc tttaaacaat atcttaatat atagctcaat agcaagtata
     1981 tgatgtcgag tggcgcgcgt tatcgcaatc ttaccctgag tctgtttacc atcgctcttc
     2041 cctttttcgc tttctcccag caacaaccgg aaaagaaaaa gaaaaaactc atcaaagaag
     2101 tatatgtctc gtggggatat aatggagagt ggtataccca cagcaccatt catatctcgc
     2161 agccttcgct ggggaacaac tacgattttg tccatgtaaa ggcgcatgat cacccgggct
     2221 ggaacgaagg gttgttcagc aaggccctgt ccattccgca atacaattac cggcttggtt
     2281 tcctgctgga tgaggagaag ggatggggtt tcgagatcaa tttcgatcat acgaagtata
     2341 ttttcgcaga tcaggatgtc aggctgaaag gcaccctggg caaacgtccc atcgacacga
     2401 ccatcgcttt taaccaggcc aatggctttt attactacct gaacaacggc gcgaatttcc
     2461 tcctcttcaa tgtgaccaag aaatggcatc tgctgaaaac ggatgatggg aaggtgaaag
     2521 tggatttcct tgggaagttt ggcgtggggc cggtgatccc gcatacgcag aacagcctgt
     2581 tcgggcatcc caaccatgaa ggcttccaat tcgggggctg gaacacgggt ctggagggtt
     2641 cgctgcgggc gaccttcttc cggtatgcct acctggaata ttccaacaag atcgactatg
     2701 cccgttactc cgggttaaat atttacgagg gcaaggcgca tcaggcgttt ggaacgtacg
     2761 agatgatcct gagcatcggg tttgttatcc caacgactgc gaagaagtaa ccggcgtagc
     2821 gggtttcttt tcgaacagcc accatttcct tttacggacg ccttcgcggt aattcttttt
     2881 tataaacgtg tcgatgtgcc ggatagcctc ggccacgctg tcggtaagca ggacgaggga
     2941 catgtcctcc ttcgatatcg ttccttggcc ggccatggat tccatatatt caaagagcgg
     3001 cgcgtagtat tctttcccaa acagcactat cggaaagcgg acgagggtct ttgtttgtac
     3061 cagcgtcaat gtttcgaaga actcgtccat ggtcccgaag ccacccggca tgatcacgaa
     3121 ggcataggag tattttacca gcatcacttt ccggacgaaa aaatggtcga aggtgatgga
     3181 tgtctgtaca taggggttgg gggcctgttc aaagggcagt tttatattac aaccgaccga
     3241 catcccgccc tgctcgaagg cgccacgatt ggcggcttcc attatacccg ggcctcctcc
     3301 tgtcatcgtc gtcaggccca gggtggctat ctggcggccc atttcccggg ccgattggta
     3361 gtgcggatgg tcctccttga agcgggccga tccaaagacg gtgatacagg ggcctacgaa
     3421 atgtagcgtt cgaaatcctt ttataaactc ccggaagact ctccaggcga agctgaactc
     3481 atatggccgg ctcttgggtc cgtcgagaaa ttgtacgtct ttggcgggta taacccggtt
     3541 taattgttcc atgcttaggc agatgtagta aatttgtgcg ctaatttaac gtaaacattc
     3601 atatgtcctc cttacgtatc gtcagctata atgttaacgg catccgggcc gccatgaaca
     3661 aggggttggt cgactggctt aagaccgacg tggccgatgt cttttgtgta caggagacaa
     3721 aggcccaccg ggacaatgtc gcgcatgccc tgttcaccga cctgggtttc catgattact
     3781 ggttccccgc gcagaagaaa ggatatagcg gggtggccgt gtttacgaag gtgaaacccg
     3841 acaatgtcgt ttatggtacg gggcatgccg tcagtgatga ggagggtcgc gtgatccagc
     3901 tggatctcgg cgatatccgg ctgatcaatg cgtattttcc atccggaact agcggggagg
     3961 aacggcaggt gtataaatac caatggctgg atgagatgca tgcctatctc gaggagctga
     4021 agaagaccca tccgcggctg gtgctttgtg gcgactataa tatcgcacac cgggagatcg
     4081 atatccatga tccaaagggc aataagaaca attccggttt tctgccggag gaaaaggcat
     4141 ggatggataa gttcatgggg agcggatgga tcgacagttt ccggcatttt tgtgcggacc
     4201 cgcatcatta tacctggtgg agtcagcgtt tcccttctgt ccgtcttgag aacaagggat
     4261 ggcggatcga ttatatcagt gttacggcgc cgttaaagca gcgggtgttg agcgcagcta
     4321 tctatcccga cgtaaagcat agcgatcact gccctgtgta cctggagctg gcggcaaaat
     4381 aaaggggcgg gcgcttttgg ataccgatct taccgattat ctttaatcgg ttatctctat
     4441 tggcgaaccc cgcaaatcca acaatggaca tcattgccat attactgctt tgtctcctga
     4501 tcatcctggt gttgctattg cgcaacagtt tgttcgatcg tctcgaccgg ctcgaaaggg
     4561 aggtctcccg attgagggag gagaaggcgg cagcgcctcc gccggaggct gtgtccgtgc
     4621 cggttgcgaa gccgcccgct gcggtgccac ccagtgaggt tcctcccatt gcgaagcccc
     4681 gggaaacgga accattccgg ccgatggagc ggccgcgacc cgtcgaggcg ccttctccaa
     4741 gaccggcagc ggcctcgacg ccgcgacccg cagaaagagc tgcgtatgcg ccgatcgagc
     4801 cgccgcattt tcagcgaatg gatgaggtga agccgccgcc ggccgcagga ccggagcccg
     4861 gctttttcga acgacatcct gacctggaaa aattcatcgg ggagaacctc atcagcaaga
     4921 tcgggattgc catccttgtg ctggcgatcg gcttttttgt aaaatatgcc atcgataacg
     4981 actgggtcgg cccggttggt cgtgttggta tcggattact ttgcgggggc atcctcatcg
     5041 ggatcgctca ttggatgcgc aaccaataca aggccttcag ctccgtgctt gtgggtggcg
     5101 gactggccgt cctttatttt accattgccc ttgcctatca ccaatatcaa ttgttcaatc
     5161 agcctacggc gtttgtgatc atgctcgtgg tgacggcttt ctccgtcctg ctttcccttt
     5221 tatacgaccg ccaggagctg gccattatcg cgctggtggg cggcttcatc actcctttcc
     5281 tcgtgagtaa cggaaggggc gattatgtcg tgctgttcgg ttacctgctt atcctgaata
     5341 cggggctgct ggtcatcgcc tataacaagg cctggcggct gttgaacatc ctttccttt
//
LOCUS       DATGFG010001140         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1009536, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001140 DATGFG010000000
VERSION     DATGFG010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009536"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VKQ52_21645"
     CDS             <1..330
                     /locus_tag="VKQ52_21645"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89879.1"
                     /translation="DKKTYQMDPANRLEAVKEAMMDVEEGADIVMVKPALPYLDIIRE
                     VKNAVHVPVSAYNISGEYALIKAAAKMGWINEEKAILETLIAIKRAGADLIATYFAKD
                     AVKLLNS"
     gene            334..1623
                     /gene="hemL"
                     /locus_tag="VKQ52_21650"
     CDS             334..1623
                     /gene="hemL"
                     /locus_tag="VKQ52_21650"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HLZ89880.1"
                     /translation="MYTRSKELFERAQQSIPGGVNSPVRAFRSVGGTPVFMQRAQGPY
                     MFDVDGNRYIDYIASWGPMILGHAWGPAVKAIQEYAALSTSYGAPTELEVEMAELIRS
                     MVPNLDLVRMVSSGTEACMSAVRLARGFTGRNRIIKFEGHYHGHADPFLVKAGSGVAT
                     FNIQTVPGVTPGVAQDTLTAAYNDLPGVERLIAAYPGAIAAIIVEPVAGNMGCILPEP
                     GFLEGLRAICDREGIVFIFDEVMTGFRLAPGGAQERLGIRADLLTFGKVIGGGMPVGA
                     FGGKKEIMQHIAPLGNVYQAGTLSGNPIAMIAGYTVLSALKANPTIYSELEEKTIYLR
                     ERLDRVFQAWGEPYRINQFGSMISIHFSDHPVRDFSSASGANNELFKLFFHSMLKRGV
                     YLPPSAFESWFLNNALTRADLDATVRAAEESLAELDG"
     gene            complement(1666..2622)
                     /locus_tag="VKQ52_21655"
     CDS             complement(1666..2622)
                     /locus_tag="VKQ52_21655"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ89881.1"
                     /translation="MATQVAYQAPDILMMAYKQPIMQDQLTLLQEREQQIPGSVQYTI
                     KRYRKHPQWSLDDMGMMVYHYKKEEATRNYLELRFCIAGNVYCQKKNIECDSCKLNAT
                     PGCSERIDTVDVLSFRFHPVQLSQFSRPRRVESTVSDDLLEFRHPGSFSKTLSLCGRT
                     RLVLETLLNHTYTGSLENIYINGQSQMLLLYSLECMVGEKEVEGFQCKFLANEADREK
                     IVQAREILLKHIGEPLTIKELSRKVAINECYLKKGFKEMFGTTIFDFYQGQRMEHARY
                     LLYEKGLSVTEVSVMLGYSSISHFSTAFKKHTGLKPCELLLH"
     gene            2843..4822
                     /gene="gyrB"
                     /locus_tag="VKQ52_21660"
     CDS             2843..4822
                     /gene="gyrB"
                     /locus_tag="VKQ52_21660"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HLZ89882.1"
                     /translation="MSETTQEVLTSAGNGYGADSIQVLEGLEAVRKRPAMYIGDIGVK
                     GLHHLVYEVVDNSIDEALAGYCKNILVTIFEDNSIEVEDDGRGIPTGINTKEQKSALE
                     MVMTILHAGGKFDKNTYKVSGGLHGVGVSCVNALSADLHVTVYREGKIFEQAYKIGVP
                     QYQVREIGNSDKTGTTVHFWPDSSIFITTEYNKDILEGRLRELSFLNRGVRITLRDLR
                     EKEEDGSTYSKTFYSEGGIVEFVDMLDKNAGRQPLIPKIIYVEGRDEVTNVAVEVALT
                     YNDSYSEHIYSYVNNINTIEGGTHVAGFRRALTRVFKAYGDREGHFEKAKVEIEGDDF
                     REGLSTIISVKVPEPQFEGQTKTKLGNNEVSGVVDSTVSKVLNTYLEENPREAKNIIN
                     KVILAAQARAAAKKARELVQRKSVLSGGGLSGKLADCSDRNPDRCELFLVEGDSAGGT
                     AKQGRDRSFQAILPLRGKILNVEKAMEHKIYENEEIRNMYTALGVTVGTPEDPKALNL
                     AKLRYHKLIIMTDADVDGSHIATLILTFIYRYMKEMVEQGYVYLAQPPLYLVKKGKEQ
                     SYAWNEDQRRAWVEKLANGKEDSVTVQRYKGLGEMNSDQLWDTTMNPETRTLKQVTIE
                     SAAEADRIFSMLMGDEVAPRRDFIESHAKYAKLDV"
     gene            4895..5098
                     /locus_tag="VKQ52_21665"
     CDS             4895..5098
                     /locus_tag="VKQ52_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89883.1"
                     /translation="MSTVTLTAYNVKTKEKNVPIQDAVITKTAKGAYMAQGHDGKGNK
                     LTTLLGEEKALAAIKEGVAKQGW"
BASE COUNT         1216 a         1213 c         1760 g         1138 t
ORIGIN      
        1 gacaagaaga cctaccagat ggacccggcc aaccggctgg aggccgtgaa ggaggcgatg
       61 atggatgtgg aggagggcgc ggatatcgtc atggtcaagc ctgcgcttcc ttacctggat
      121 attatccgcg aggtgaagaa tgcggtgcat gtgccggtga gcgcctacaa tatcagcggg
      181 gaatatgccc tgataaaggc ggcggcgaag atgggatgga tcaatgaaga aaaggctata
      241 ttggagaccc ttatcgcgat caagcgggcc ggggccgacc tgatcgcgac ctatttcgcg
      301 aaggatgcgg tgaaattgtt aaactcttag accatgtata cgcggagtaa agaacttttt
      361 gaaagggcgc agcagagtat tcccggtggg gtcaactccc ctgtccgggc gtttcgcagt
      421 gtcggcggga cgccggtgtt catgcagcgg gcgcagggac cgtacatgtt cgatgtggac
      481 ggcaaccggt atatcgatta tatcgcttcc tgggggccga tgatccttgg gcatgcctgg
      541 gggcctgccg taaaggcgat acaggaatat gcggcgcttt ccacgtccta tggcgcgcct
      601 acggagctgg aggtggagat ggcggagctg atccgcagca tggtccccaa cctcgacctg
      661 gtgaggatgg tcagcagcgg taccgaggcg tgtatgagtg ctgtccggct ggcgaggggt
      721 tttacggggc ggaacaggat catcaaattc gaggggcact atcacggtca tgccgatccg
      781 tttttggtaa aggcgggcag tggagtggct actttcaata tccagacggt gcccggggtt
      841 acgccggggg ttgcgcagga tacgctgacc gcggcttata acgatctgcc gggggtcgag
      901 cggctgatag cggcctatcc cggggccatt gcggccatta tcgtcgagcc cgtggcgggc
      961 aatatgggtt gtatcctgcc cgagccggga ttcctggagg ggttgcgggc gatctgcgac
     1021 cgggagggga tcgtattcat cttcgacgag gtgatgacgg gcttccggct ggcgccgggt
     1081 ggcgcgcagg agcggttggg tattcgcgcc gatctgctca cgtttgggaa ggtgatcggc
     1141 ggggggatgc cggtgggggc gtttggcgga aaaaaagaga tcatgcagca tatcgctccg
     1201 ctgggaaatg tctaccaggc gggaacgctg agtggcaacc cgatcgcgat gatcgcgggg
     1261 tataccgttc tctcggcgtt aaaggcgaac ccgactatct acagtgaact ggaggagaag
     1321 acgatctatc tgcgggagcg gcttgacagg gtgttccagg cctgggggga gccgtaccgg
     1381 atcaaccaat tcggatcgat gatcagtata catttcagcg accatcccgt gcgggatttc
     1441 agttcggcgt cgggtgcgaa taacgagttg ttcaagctgt tctttcattc gatgctgaag
     1501 cggggtgttt acttgccgcc ttcggcgttc gagagctggt ttttgaacaa tgcgctgacg
     1561 agggccgacc tggatgcgac ggtgcgggcg gcggaggaga gtcttgccga gctggatgga
     1621 tgatcgtgag cggggccggg tcgatgttcc tgcgccgggt gggaatcagt gcagcaatag
     1681 ttcacagggt ttgaggcccg tgtgtttctt gaaggcggtg gaaaaatggg agatggagga
     1741 gtagccgagc atgacggaga cctcggtgac gctcaggcct ttttcgtaga ggaggtagcg
     1801 ggcgtgttcc atgcgttgtc cctgatagaa gtcgaagatg gtggtgccga acatttcttt
     1861 aaagcccttt ttcagatagc attcgttgat ggccacttta cggctgagct ccttgatggt
     1921 gaggggttcg ccgatatgtt tgaggaggat ctcgcgggcc tggacgatct tttcccgatc
     1981 ggcttcgttt gccaggaatt tgcactggaa gccttctact tctttttccc ctaccatgca
     2041 ttcgagacta tagagcagga gcatctggct ctggccgttg atatagatat tctccaggct
     2101 gcccgtatag gtatggttca gcagggtctc gaggaccagc cgggtccttc cgcaaagaga
     2161 gagggtcttg gaaaaggagc cggggtgccg gaattcgagc aggtcatcgc tgacggtgct
     2221 ttcgacgcgc cggggccggc tgaactggga aagctggacg gggtgaaagc ggaagctgag
     2281 gacgtccacg gtgtcgatgc gttcggagca gcctggtgtg gcgttcagct tacaggaatc
     2341 gcattcgatg ttctttttct ggcaatagac gtttccggcg atgcagaaac ggagttcgag
     2401 ataattgcgg gtggcttctt cctttttgta gtgatagacc atcatcccca tgtcatcgag
     2461 tgaccattgg ggatgtttcc ggtagcgttt gatggtgtat tgaacggagc cggggatctg
     2521 ttgttccctt tcctggagga gggtcaactg atcctgcatg atgggttgtt tgtatgccat
     2581 catcaaaata tccggagcct ggtacgccac ctgtgttgcc attggtcaaa attagatcga
     2641 aaaaggcgaa taaattcagg ttggccggcg cggggggtca aaatgggggt ttttgacaga
     2701 attatgacgt taaaatcaag cgcgaaaatt atcacaaaac gggtcgaaat ggggatatta
     2761 aaacctcggg aaccccttat ttttcgtaca tttgcgaacc ttattaattt attgttaact
     2821 acactattcc agacgaaaaa atatgagtga aacaacacag gaagtcctga cctcggccgg
     2881 caacggatat ggggcagaca gcatccaggt actcgaaggt ttggaggcgg ttcgcaagcg
     2941 tccagccatg tatatcggtg atatcggcgt caaaggcctg catcacctgg tatacgaggt
     3001 ggtggacaac tccatcgacg aggctctggc gggatattgt aagaacatcc tggttaccat
     3061 ttttgaagac aattccatcg aggtggagga tgacggtcgt ggcattccca cggggatcaa
     3121 caccaaggag cagaagagtg cgctggagat ggtgatgacc attcttcatg ccgggggtaa
     3181 attcgacaag aacacgtata aggtgtccgg cgggcttcac ggggtagggg tcagttgtgt
     3241 gaatgcgttg agcgccgacc tgcatgtaac ggtgtaccgc gaaggcaaga tcttcgagca
     3301 ggcgtataag atcggggtac cgcagtacca ggtgcgggag atcggcaaca gtgataagac
     3361 gggcactacg gtgcatttct ggcccgattc ttctattttt atcacgacgg agtacaacaa
     3421 ggatatcctg gagggaaggc tccgggagct gtcttttttg aaccggggtg tacggatcac
     3481 gctgagggac ctgcgggaga aggaggagga tggcagcacg tacagcaaga ccttttacag
     3541 tgagggcggg atcgtcgagt ttgtggacat gctggacaag aatgcgggcc ggcagccgct
     3601 gatcccgaag atcatctatg tggagggccg ggatgaggtt acgaatgtgg cggtggaagt
     3661 ggcgcttacc tacaacgaca gctacagtga gcatatctat tcttacgtaa acaatatcaa
     3721 cacgatagaa ggcggcaccc acgtggccgg ctttcgccgg gcgctgaccc gggtgttcaa
     3781 ggcctatggg gaccgggaag ggcatttcga gaaggcgaag gtcgagatcg agggggatga
     3841 tttccgggag gggttgagca cgatcatatc ggtcaaggtt cccgagccgc agttcgaggg
     3901 tcagacgaag accaaactgg ggaacaacga ggtgagcggg gttgtggatt ccaccgtcag
     3961 caaggtactg aatacctacc tcgaggagaa tccccgggag gcgaagaata tcatcaacaa
     4021 ggtgatcctg gcggcccagg cgagagcggc ggcgaagaag gcgcgggagc tggtacagcg
     4081 gaagagtgtg ctcagcggcg gtggtctttc gggtaagctg gccgactgtt ccgatcgtaa
     4141 tcctgaccgt tgcgagttat tccttgtcga aggtgactcg gcgggtggta cggccaaaca
     4201 gggccgggac cgcagctttc aggccatcct cccgttgcgg ggtaagatcc tcaacgtaga
     4261 gaaggccatg gagcataaga tctatgagaa cgaggaaatc cgcaatatgt atacggccct
     4321 tggggtgacg gtgggtacgc cggaggatcc caaggcgttg aatcttgcca agttgcgtta
     4381 tcacaagctg atcatcatga ccgatgccga tgtggatgga agccatatcg cgacgctgat
     4441 cctgaccttt atctaccggt acatgaagga gatggtggag cagggctatg tttacctggc
     4501 ccagccaccg ctgtatctcg tgaagaaggg caaggagcag tcctatgcct ggaacgagga
     4561 ccaacggcgg gcctgggtag agaagcttgc caacggcaag gaagacagcg tcacggtaca
     4621 acggtataag ggtctggggg agatgaactc cgaccagctg tgggacacga cgatgaaccc
     4681 ggaaacgcgg acgttgaaac aggtgacgat cgagagtgct gccgaggccg atcgcatctt
     4741 cagcatgctg atgggggatg aggtggcgcc ccggcgggat ttcatcgaat cgcacgccaa
     4801 gtatgcgaaa ttggacgtgt agatgaaaaa ccttaatatt acattgtttt catactaacc
     4861 agacctgcca tagcaggcat taaatcaatg aattatgtca actgtaacac ttacggccta
     4921 caacgtcaag acgaaagaga agaatgtgcc tatccaggac gccgttatca cgaagaccgc
     4981 gaagggtgca tatatggcgc aggggcatga tggcaagggc aacaagctga cgaccctgct
     5041 gggcgaggag aaggccctgg ctgcgatcaa ggagggtgtt gccaagcaag gctggtagac
     5101 accggtcatt ttacaaaacc gtagacctta cagaacagaa attcccccat tcccatagta
     5161 aaaaaatgat gatcgctttc cgcggcagta acggatggcg ccggcgcgtt aacggatggc
     5221 gtcggcgccg aagtaggtgt gcagtgccgg tgggagcgcg ataaagccgt ccttttggtg
     5281 attctccagc aggcaggcca cgatgcgggg aagcgcgagg gaggacc
//
LOCUS       DATGFG010001141         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1506449, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001141 DATGFG010000000
VERSION     DATGFG010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506449"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VKQ52_21670"
     CDS             complement(<1..1541)
                     /locus_tag="VKQ52_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89884.1"
                     /translation="MVYISHRQQQERNREKPFFYPALLRLAACLRRTTLPCLAAFTLL
                     ASQTTVAQELIVQVNKPGAPIKPTMWGIFFEDINLAADGGIYAELVKNRSFEFTTPLM
                     GWTEHAATPGAGSILVLNRSAEKPDNPRFIRVTHYTDAAYTLTNEGFRGMGIRQGETY
                     HFSIWNRPLPGAGITLRLELQTATGAVIGSADLALTNTGDWKKDTISFTATTTEPKAH
                     LTIGFEGQGAVDLDMISLFPEHTWKQRPGGLRADLVQLLADMHPGFIRFPGGCIVEGR
                     DLTNRYQWKKTVGRVEDRKLIINRWNTEFAHRSTPDYFQSFGLGFFEYFQLAEDIGAE
                     PLPILNCGMACQFNTSEVVPIPDIDPYIQDALDLIEFANGDASTKWGGLRAAMGHPQP
                     FHLKMMGVGNEQWGPQYIERYHVFTKAIKARYPDIHLVNSTGPDPNGARFDFLNDTLR
                     KLHADILDEHYYRKPEWFLENATRYDHYDRNGPKIFAGEYAAQSDKTVSVDNHNNWQC
                     ALAEAA"
     gene            1566..1910
                     /locus_tag="VKQ52_21675"
     CDS             1566..1910
                     /locus_tag="VKQ52_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89885.1"
                     /translation="MDEKSTDQLLLFGQFIDFYYPSIYSAITKLTGLTDKKELEAITM
                     EVFDELWKTREQVLNELRPPAFIYKILIRHVFAYLRKQGHEDRIMALQNFLYSLPDDD
                     GPATDEGKPLHP"
     gene            complement(1854..>2359)
                     /locus_tag="VKQ52_21680"
     CDS             complement(1854..>2359)
                     /locus_tag="VKQ52_21680"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HLZ89886.1"
                     /translation="PWLDFGSFWDGIKLTKLTPDARSLAEPQQWYTIASRHRTGTIPD
                     TAAGDAAIEAPFICKKDNYYYLFVSFDYCCRGEKSTYNIRVGRAENIQGPYLDRDGTP
                     MTLGGGSLVLEGDRNWNGVGHCAVVEFPDGDWLVFHGYDANDRGRSKLRMERLAFISG
                     WPVIIRQ"
BASE COUNT          459 a          552 c          791 g          557 t
ORIGIN      
        1 gcggcttccg ccagggcgca ttgccagttg ttgtgattat ccaccgaaac cgtcttgtca
       61 ctttgcgcgg cgtattcgcc ggcgaagatc tttggaccgt tgcgatcgta atggtcataa
      121 cgggttgcat tctccaggaa ccattcaggt tttcgatagt aatgctcgtc gaggatgtcg
      181 gcgtgcagtt tacgcagcgt atcattcagg aaatcgaacc gggcgccatt ggggtcgggg
      241 cccgtgctgt tgacgagatg gatatcgggg taacgggctt ttatggcttt tgtgaagaca
      301 tggtagcgtt cgatgtattg cggtccccat tgttcgttgc ccacgcccat cattttgaga
      361 tgaaagggtt gggggtggcc catggctgcg cggaggccgc cccatttggt ggaggcgtcg
      421 ccgttcgcga actcgatgag atcgagggcg tcctggatat aggggtcgat atcggggatg
      481 ggcactactt cggaggtgtt gaactggcag gccatgccgc agttgaggat gggcaggggt
      541 tcggcgccga tgtcttcggc cagctggaaa tattcgaaga agccgaggcc gaaggactgg
      601 aagtagtcgg gggtggagcg gtgggcgaac tcggtattcc agcggttgat gatcagtttg
      661 cggtcctcta ccctaccgac cgttttcttc cactggtagc gattggtcag gtcgcggcct
      721 tcgacgatgc agccgccggg gaagcggata aagccggggt gcatgtcggc gagcagctgg
      781 accagatcgg cgcggaggcc gccgggtcgt tgcttccagg tatgttcggg gaagagggag
      841 atcatgtcga gatcgacggc gccctggcct tcgaagccga tggtgagatg ggcttttggt
      901 tcggtggtag tggccgtgaa ggagatggtg tcttttttcc agtcgccggt gttggtcagg
      961 gcgagatctg ccgaaccgat aacggcgccg gtggcggttt ggagttcgag gcggagggtg
     1021 atccccgcgc cggggagggg gcggttccag atggagaaat gataggtctc gccctggcgg
     1081 atgcccatgc cgcggaagcc ttcgttggtg agggtatagg cggcgtcggt gtaatgggtg
     1141 acgcggatga agcgggggtt atcgggtttc tccgccgagc ggttaaggac gaggatggag
     1201 ccggcgccgg gggtagcggc gtgttcggtc cagcccatga ggggggtggt gaactcgaag
     1261 gagcggttct ttaccagttc ggcatagatg ccgccgtcgg cggcgaggtt gatgtcttcg
     1321 aagaagatgc cccacatagt gggttttatg ggggcgccgg gtttattgac ctggactata
     1381 agctcctggg cgacagttgt ttggctagcc aggagggtga aggctgcgag gcatggcaag
     1441 gttgtcctgc ggaggcaggc ggccaggcgg agcaaggcgg gataaaaaaa tggcttttca
     1501 cggttacgtt cctgttgctg gcgatgacta atgtaaacca ttgtacctta ttaaaattta
     1561 gcgacatgga tgaaaagtcg acggatcaat tgctattgtt cggacaattc attgattttt
     1621 actatccttc tatttattcg gcgatcacca agcttaccgg tcttacggat aagaaagagc
     1681 tggaagcgat caccatggaa gtgtttgacg aattatggaa gacgagggag caggtgctga
     1741 acgagttgcg tcccccggct tttatctata agatcctgat ccggcatgtt tttgcttacc
     1801 tgcggaagca gggtcatgag gaccggatca tggctttaca gaatttcctg tattcactgc
     1861 ctgatgatga cgggccagcc actgatgaag gcaagccgct ccatccttag ttttgatcgt
     1921 cccctgtcat tggcatcgta gccgtgaaag acgagccagt cgccgtccgg gaattccacg
     1981 acggcgcagt gccctacccc attccaattc ctgtctcctt ccagcaccag cgaaccgccg
     2041 cccagtgtca tgggggtgcc gtcgcgatcc agataaggcc cctgtatatt ttctgcccgt
     2101 cctaccctga tattgtaggt actcttctcc ccacggcaac aatagtcgaa agatacgaat
     2161 agataataat aattgtcttt tttacaaata aaaggcgctt cgatggcggc atcgccggcc
     2221 gcggtgtcgg gaatggtccc ggtgcgatgg cgggaggcga tggtgtacca ttgctggggt
     2281 tcggcgaggg atcgtgcgtc gggggtcagc ttcgtcagtt ttatgccgtc ccaaaaggag
     2341 ccgaagtcca gccaggggg
//
LOCUS       DATGFG010001142         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_755338, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001142 DATGFG010000000
VERSION     DATGFG010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755338"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VKQ52_21685"
     CDS             <1..708
                     /locus_tag="VKQ52_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89887.1"
                     /translation="GKGGLITGNLRPGVRQYLVTYQDPGKDRQLRFWYWVREITTGRQ
                     NGQPVFFINQRWYGSDTGAYRTVYSVNDAGSFAPVYHRELVRGKLNAYNWQRDGIAGA
                     DSVEGNARNGFSLAFSEPNLNWNLDIETFEMLPLAAGKSFLINFYDAGLDPPQYVRYT
                     VRGSERLALQEGGQVDCWVLVTEGKDPKGATYTETYWISKKGHEFLKEVDHYNGTIRY
                     KILMPAATPDVRSGFTP"
     gene            944..2329
                     /locus_tag="VKQ52_21690"
     CDS             944..2329
                     /locus_tag="VKQ52_21690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HLZ89888.1"
                     /translation="MSKITLKRFLPFILLAIVAVAAIFIPSLTPSVDDGKYNSADVTW
                     LLVATALVFLMTPALAFFYGGMVNRKNVLSTMMKSVVAAGVVGVLWIVVGYSLCFGES
                     IHGVIGNPLTHLFYKGVTSGPVWGPAPTIPKALFSMFQLMFAIITPGLVVGAIAERMR
                     FSAYVLFTVLFSLLVYAPLAHWSWHPDGFLAKMGALDFAGGTVVHISAGCAALAGALV
                     LKRRKVHIERQETHPANIPYVLIGTGLLWFGWFGFNAGSALAANSLAVSAFSTTNTAA
                     AAAGLSWMFFDVMRGKKPSVVGFCVGAVVGLVAITPAAGYVAVPQSIFIGVFAAVVSN
                     MAVHFKATKTVLDDTLDVFPCHGLGGMVGMLLTGVFATLTVNKAGANGLLNGNAGFFF
                     TQLKAMLIAVGYSFAVSYGIFKFINFIVPLRVSQEEEEEGLDASQHDEKYTQGTLLVA
                     TPKGLEEHEIM"
     gene            2465..3010
                     /locus_tag="VKQ52_21695"
     CDS             2465..3010
                     /locus_tag="VKQ52_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89889.1"
                     /translation="MQNDWSSFRLRVNIDVLPAKVYAAWATRAGLESWFLRRGLATVG
                     EAGRSPRGEDEPTRGAGKLPRGDDEPLQKGDRYAWQWHGYPDEVEHSGTVMAANGTNQ
                     FAFTFTMGCLVNISIFRECEETIVELMESGLPVDEETLLKRYVEDYKGWIFYLVNLKS
                     VLEGGLDLRNRKQELTNVITA"
     gene            complement(3109..3966)
                     /locus_tag="VKQ52_21700"
     CDS             complement(3109..3966)
                     /locus_tag="VKQ52_21700"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLZ89890.1"
                     /translation="MIARDLPPPAIFSASGRPLVFADHLLVTGITDAPYSLPEHVPGM
                     GLLSMLAGNGACTINGRRESLHTSQYLLINRGSRISITLPHPGAQPLFLFFHTALADE
                     VEAGMGVDWRWLERVHPLPQQLRQRLEWLVSSGRNCSSFGALQADSMIRGVLDGLFHE
                     ARMAAALSQDLPVARQATRVELFKRLSLAREWIAANYASPVTVDDMARVAALNPQHFL
                     RMFRDCYGKTPHRFLTDTRLEAARQMLLHTGESIVAICRQTGFESPSTFSGLFRKRFG
                     RPPAIFRRK"
     gene            4017..>4328
                     /locus_tag="VKQ52_21705"
     CDS             4017..>4328
                     /locus_tag="VKQ52_21705"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HLZ89891.1"
                     /translation="MISQQVLVEARKLWDYHHLNHIPAPSDCILVLGSHDLRVADRGA
                     DLYLQGLAPILIFSGGLGNVTKGIWKDPEADRFARIALDKGVPAEAIFIENQSTNTGE
                     NI"
BASE COUNT          859 a         1093 c         1355 g         1021 t
ORIGIN      
        1 gggaaggggg gattgatcac cggcaatctc cggccgggcg tgcggcaata tctcgtcact
       61 taccaggacc cgggcaagga ccggcaactg cgtttttggt attgggtacg ggagatcacc
      121 accggccggc agaacgggca acccgtcttt tttatcaacc agcgctggta tggcagcgat
      181 accggggctt accggacggt ctactcggtg aatgacgcgg gcagttttgc gcctgtgtat
      241 catcgtgagt tggtgcgggg gaaattgaat gcctataact ggcagcggga cggcattgcc
      301 ggcgccgaca gcgtggaggg taatgcgcgt aatgggttct cccttgcgtt cagcgagccg
      361 aacctcaact ggaacctcga tatcgagacc tttgaaatgc taccgctggc cgccggcaag
      421 tcgttcctga tcaatttcta tgatgcgggt ctcgacccgc cgcaatatgt ccgctatacc
      481 gtgcggggca gtgagcgact ggccttgcag gagggcgggc aggtggattg ctgggtcctg
      541 gtgacggaag gaaaggaccc gaagggggcg acctataccg agacctattg gatcagcaag
      601 aaggggcatg aatttttgaa ggaggtggat cattataatg ggacgatccg gtacaagatc
      661 ctgatgccgg cggccacccc cgatgtgcgg agcgggttta ccccctaaga tgggatagag
      721 aaattcaaaa aacaggccct ttttttagtt aaattgtaaa atacttatga accagggagt
      781 tgcacagtgg gttttagccg ggtgattatt aggtggttat atcatggttt tataaacatt
      841 aattaaaatg gatatacgtt aacgcatatc cattttgctt ttttggtgta tctttattgc
      901 gtaacataat gtattaattt ttaattaata tgaaaacgaa actatgagca aaatcactct
      961 taaaagattc ttacccttca tcctgctggc tattgtagcg gtggcggcga tcttcattcc
     1021 cagtcttacc cccagcgtcg atgatggtaa gtataattca gcggatgtta cctggctgct
     1081 ggtagcgacg gccctggttt tcctgatgac tccggccctg gccttctttt atggaggcat
     1141 ggtgaaccgg aagaatgtcc tttccacgat gatgaagagt gtcgtagcgg ctggcgtggt
     1201 cggggttctg tggatcgtgg tcggttatag tctttgtttt ggcgagtcta tccatggcgt
     1261 tatcggcaac ccgctgacgc atttgtttta taaaggcgtg acctcgggtc cggtatgggg
     1321 accggcgcct accatcccca aagcgctgtt ctcgatgttc cagctcatgt tcgccatcat
     1381 cacccccggg ttggtggtgg gcgccatcgc cgagcggatg cggttctccg cctatgtcct
     1441 tttcaccgtg ttgttcagcc tgctggtcta tgcgccgttg gcgcactgga gctggcatcc
     1501 cgatggcttc cttgccaaga tgggggcgct tgacttcgcc ggtggtacgg tggtacatat
     1561 ctccgccggt tgtgcggctc tcgccggggc gctggtattg aagcgccgga aggtgcatat
     1621 cgaacgccag gagacgcatc cggccaacat tccctatgta ttgatcggta cggggttgct
     1681 gtggtttggc tggttcggct ttaacgccgg ttccgccctg gccgccaatt ctcttgccgt
     1741 atccgctttc tccaccacga acacggctgc tgccgccgcc ggtttgtcct ggatgttctt
     1801 cgatgtcatg aggggcaaga agccttcggt ggttggtttc tgtgtgggcg ccgtggttgg
     1861 tcttgtagcg atcacgcccg ctgccggcta tgtagccgtt ccgcaaagta tctttatcgg
     1921 cgtatttgcc gccgttgttt cgaatatggc cgttcatttc aaggctacca agaccgttct
     1981 ggacgatacg ctcgatgtgt tcccctgtca cgggcttggc gggatggtcg ggatgttgct
     2041 caccggagta ttcgccacgc tgacggtcaa caaggccggc gccaatggtc tgctcaacgg
     2101 taatgccggt ttcttcttca cccagctgaa ggccatgctg atcgccgtcg gctacagctt
     2161 cgcggtttct tacggcatct tcaagttcat caacttcatc gttcccctgc gggtcagcca
     2221 ggaagaagag gaagaagggc tggatgccag ccagcacgat gagaagtaca cgcagggtac
     2281 cctgctggtg gctacgccga aagggctgga ggagcacgag atcatgtaag atagctttct
     2341 catattagtg aagggccggc tttgccggcc ctttttttgt ttttccccat cccatcctca
     2401 ttttctcatt tttgaagaag ccggtttggc gccatcgccg taaccttgtc ggaaaatcac
     2461 ttttatgcaa aacgattgga gcagttttcg cctgcgggtg aatattgatg ttctgccggc
     2521 gaaggtctat gcggcctggg ccacgagggc ggggctggag tcctggtttc tgcgccgggg
     2581 tcttgcgact gtcggggagg ccggcaggtc gccccggggg gaagacgagc cgaccagggg
     2641 cgcgggaaaa ttgccgcggg gggacgacga gccgctgcaa aaaggcgacc gttatgcctg
     2701 gcaatggcat ggctatcccg acgaggtgga gcattccggg acggtcatgg ccgccaatgg
     2761 tacgaaccag tttgcgttta cttttacgat gggttgcctg gtgaacatca gcattttccg
     2821 ggaatgcgaa gaaacgatcg tggagttgat ggaaagcggg cttccggtgg acgaggagac
     2881 gctgttgaaa cggtatgtcg aggactacaa gggatggatc ttttatcttg tcaacctgaa
     2941 atcggtgctc gaagggggat tggatctgcg caaccggaaa caggagctga cgaatgtgat
     3001 cacggcctga acggatgggg tgcgggtggc tgagatgggt gcggatggcc gagatggggt
     3061 tcaggtggct gagatggagt gcggctggcc ggggcggcat gctgcggctt actttctccg
     3121 gaagattgcc ggcgggcgcc cgaatcgctt tcggaacagg ccgctaaagg tagacgggct
     3181 ttcgaagccg gtctggcggc agatggcgac gatactctcg ccggtgtgca gcagcatctg
     3241 ccgtgccgct tccagccttg tatcggtgag aaagcggtgt ggcgttttgc catagcaatc
     3301 gcggaacatc cgcaggaaat gctgggggtt gagtgcggcg acccgggcca tgtcgtcgac
     3361 ggtgaccggg gaggcataat tggcggctat ccattcccgc gcgagggaca gccgcttgaa
     3421 caactcgacc cgggtcgcct gccgggcaac gggtaggtcc tgcgagagcg cggccgccat
     3481 ccgggcctcg tggaaaaggc catcgaggac gccccggatc atgctatcgg cttgcagcgc
     3541 gccaaaggac gaacagttcc tgcccgatga taccagccat tcgagacgct gccgcagctg
     3601 ttgcggcagg ggatgtactc tctccagcca tcgccagtcg actcccatac cggcttccac
     3661 ttcatccgcc agggcggtgt ggaagaaaag gaacagcggt tgtgctccgg ggtgggggag
     3721 ggtgatggag atccggctgc ccctgttgat cagcaggtat tggctggtat gcagtgattc
     3781 gcgccggcca ttaatagtac aggcgccgtt gcccgccagc atgctcagca ggcccatgcc
     3841 gggtacatgt tcgggaaggg aatacggcgc atccgttatc ccggtcacca gaagatggtc
     3901 ggcaaaaacc agggggcgtc cgctggcgga gaagatagcc ggagggggca aatcccgggc
     3961 gatcatggga ggcttttttc aaatttatgt aaaaatgata tctttaggcc tcctatatga
     4021 tatcgcaaca agtacttgta gaagccagaa aattgtggga ctatcatcat ctgaatcata
     4081 ttccggcccc atcggactgc atccttgtat tgggcagcca tgatcttcgt gtggctgacc
     4141 gcggtgcgga cctctatctc cagggcctgg cgcccatcct tattttttcc ggcggattgg
     4201 gtaatgtcac gaaggggatc tggaaggacc ccgaggcgga tcgttttgca cgtatcgccc
     4261 tcgacaaagg ggtgccggcc gaggccatct ttatcgagaa ccaatccacc aatacaggtg
     4321 agaacatc
//
LOCUS       DATGFG010001143         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_640535, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001143 DATGFG010000000
VERSION     DATGFG010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640535"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VKQ52_21710"
     CDS             <1..216
                     /locus_tag="VKQ52_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89892.1"
                     /translation="DSWAIQLPKSNVLQFILADGSKISARPSGTEPKIKFYFSVHTEL
                     ASPGEFDKINGELSARIDGIINDMKLK"
     gene            complement(595..>2270)
                     /locus_tag="VKQ52_21715"
     CDS             complement(595..>2270)
                     /locus_tag="VKQ52_21715"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HLZ89893.1"
                     /translation="AKISQSAGGIGLSIHNIRATGSYIGGTNGTSNGIIPMLRVFNDT
                     ARYVDQGGGKRKGAFAIYLEPWHADVFEFLDLRKNHGKDELRARDLFYALWIPDLFMQ
                     RVEAGGDWSLFCPHEAPGLHETWGAEFEQLYTQYEAEGRARRTVKAQDLWFAVLDAQI
                     ETGTPYLLYKDSANRKSNQQNLGTIKSSNLCTEIIEYTSADEIAVCNLASLALPRFVI
                     NGQFDHQKLYEVTYEVTKNLNKIIDYNYYPVEEARNSNTRHRPIGLGVQGLADAFILL
                     RLAFESDKAKQLNKEIFETIYFAAMTASKDLAKIAGAYESFAGSPTSKGQFQFDLWGV
                     EPTLRWDWYRLKDEVIKYGVRNSLLVAPMPTASTSQILGNNECFEPYTSNIYVRRVLS
                     GEFVVVNKHLLKDLVDLNLWNEDMKNRIIFHNGSIQAIDEIPQEIKDIYKTVWEIKQR
                     NIIDMAADRGAYICQSQSLNLFVDTPSASKLTSMHFYGWKKGLKTGMYYLRTQAASQA
                     VQFTVERQGGKMIEPLANPAAIQVASADAGEDMIGATCTMQDGCVTCSA"
BASE COUNT          454 a          572 c          717 g          527 t
ORIGIN      
        1 gattcctggg ccattcaact acccaaatcg aatgtgctgc agttcatcct cgcggatggc
       61 agtaagatct ctgcccggcc ttccggcacg gaacccaaga tcaaattcta tttcagcgta
      121 cataccgaat tggcttcgcc gggtgagttc gacaagataa acggtgagct atccgcgcgg
      181 atagatggga tcatcaacga catgaaactg aaatagcgaa aggtaaacca gggggctccg
      241 aaaaggggcc ttttttctta gaaagccatc tgaataattg ctacgaggac gaagagactt
      301 gcgctgagga gattggtacg tgtaaacata acggtttttt ttatagggtt acagataata
      361 gctaagagct aaagtagcta caaagaaccg catttgcaat agtaaaaaca cttaggcccc
      421 gccgaaaaaa ttggaccggg cctttcccgg taaatttacg gcccggtcca atttggctaa
      481 tgatggccct tcgggcggcg gatctgtgat ccgccctgtt cgacggggcc tgttcgttgg
      541 agcctattcg atggcgcctg gtcgatggcg ccttatagta aatcattcac tgcgttatgc
      601 gctgcaggtg acacagccgt cctgcatggt gcaggtggcg ccgatcatgt cttcgccggc
      661 atcggcggaa gctacctgga tcgcagcggg gttggcaagc ggttcgatca tcttgccgcc
      721 ctggcgttcg acggtgaact gcacggcctg ggatgcggcc tgcgtacgca ggtagtacat
      781 gccggtcttc agcccctttt tccacccgta gaaatgcatc gacgtcagct tgctggcgga
      841 tggggtatcc acgaagaggt taagtgattg cgactggcag atataggcac cgcggtcggc
      901 agccatatcg atgatattgc gctgtttgat ctcccaaacg gttttgtaga tatcctttat
      961 ctcctgcggg atctcatcga tggcctggat ggagccgttg tggaagatga tccggttctt
     1021 catgtcctca ttccacaggt tgagatcgac caggtccttc agcaggtgtt tgttcacgac
     1081 cacgaattcc ccgctgagca cgcggcgtac atagatattg gacgtgtacg gctcgaagca
     1141 ttcgttatta cccaggatct gagaagtgga cgccgtgggc atgggcgcga ccagcagcga
     1201 attacgaacg ccgtatttga tgacctcatc cttcagccgg taccagtccc agcggagggt
     1261 cggttccaca ccccagaggt cgaactggaa ctgccccttt gaggtgggcg agccggcaaa
     1321 gctctcatag gcgccagcga tttttgccag gtctttcgat gcggtcatcg cggcgaaata
     1381 gatggtctcg aagatctcct tgttcagctg ttttgcctta tcgctttcga aggccaggcg
     1441 cagcaggatg aaggcgtcgg ccaggccctg gacaccgagg ccgatgggcc ggtggcgcgt
     1501 gttggagttg cgggcctctt cgacggggta gtagttgtaa tcgatgatct tgttgaggtt
     1561 cttggtcacc tcgtaggtga cctcgtataa tttctggtgg tcgaactggc cgttgatgac
     1621 gaagcggggc agcgccaggg acgcgaggtt acagaccgcg atctcgtcgg cggaggtgta
     1681 ttcgatgatc tcggtacaga ggttggagct cttgatggta ccgaggtttt gctggttgct
     1741 cttgcggttg gcggagtcct tatacagcag gtagggggtg ccggtctcga tctgggcgtc
     1801 gaggacggcg aaccacaggt cctgggcctt tacggtgcgg cgggcgcggc cttcggcctc
     1861 gtattgcgtg tagagttgtt cgaattcggc gccccaggtc tcgtggaggc ccggtgcctc
     1921 atgggggcag aacaggctcc agtcaccgcc ggcttcgacg cgttgcatga acaggtcggg
     1981 gatccagagg gcgtagaaga ggtcgcgggc gcgcagttcg tccttcccgt ggttcttccg
     2041 aaggtcgagg aattcgaaga cgtcggcatg ccagggttcg agatagatgg cgaaggcgcc
     2101 cttgcgctta ccgccgccct ggtcgacata gcgggcggtg tcgttgaaga cgcgcagcat
     2161 ggggatgatg ccgttgctgg tgccgttggt gccgccgata taggacccgg tggcgcggat
     2221 attatggatg gagaggccga tgcccccagc agactgggat attttcgcgg
//
LOCUS       DATGFG010001144         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1095966, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001144 DATGFG010000000
VERSION     DATGFG010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095966"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..372)
                     /locus_tag="VKQ52_21720"
     CDS             complement(154..372)
                     /locus_tag="VKQ52_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89894.1"
                     /translation="MKPNVVSVTTPTASSPLTVLPTAKIVRHPRSKKEPEAALLPPPD
                     EETLNRLNVLARQYPFCRLHKGSNSPLL"
     gene            complement(709..1854)
                     /locus_tag="VKQ52_21725"
     CDS             complement(709..1854)
                     /locus_tag="VKQ52_21725"
                     /inference="COORDINATES: protein motif:HMM:NF014926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase domain 4-containing protein"
                     /protein_id="HLZ89895.1"
                     /translation="MKKLLPYALSLFCGIPILSQAQAIRASLLPMQGALRVTNDSLRR
                     NYTNITLEWQLLVNGIPKQKGTVPGISLLPGQPRTIRLPLRPLTGGEEAFLKVGFRRP
                     PQPGPPIATQQLKWKTWGGDIAIPAAGDLSFTDSNDVFTVSSPTLRLSFDKQTGWIQQ
                     YEVNHIPFLADTNGLRPALQEPPHFQLFSTSTGSQMVIVRTEYTLPGISCLLHLSYTV
                     NAAGAMLVEQTLETDTTRQDSLPHTIARFGMDWVLPPGADSLAWYGIAPEDLPDTLLP
                     TLHHRYLPATFGQNPAAPIALLVRWWTLTDRAGRGFQLTADSNLLGHITATTPPIESR
                     LRQLYPTSTQLQIDDTAWSRVLPAAHFFHYHYAFRTTPITPAPPAHR"
     gene            1961..2989
                     /locus_tag="VKQ52_21730"
     CDS             1961..2989
                     /locus_tag="VKQ52_21730"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLZ89896.1"
                     /translation="MKKVSISDIARKAGVSVSTVSFVMNDKAVKMRISREVIEKVENV
                     AREMGYRPNQLARGLRTGKTKTIGLIVENISNAFFATLAKSIEDEAKRYDYKVVYCST
                     DNDEEKARELINMLSQRQVDGYIITPTLNLADDIRKLQAENKPVILIDRYFPQHEEIP
                     SVLVDNYEGVSRGMETLIAKGYRKIALVSIETEMAHMKDRLRAYCDVLKRHGISEEHR
                     LVKIIPYNSDHERALAEVESLLSGAGSDIDAVFFLTNYLGVLGIEVIKKLQIKVPEQL
                     AVICFDDNDIFRLYTPTISVIRQPIEEIGKRAMFALVERLRHSGEESFPVNIPVRLTA
                     DLVTRESI"
     gene            3072..>3679
                     /locus_tag="VKQ52_21735"
     CDS             3072..>3679
                     /locus_tag="VKQ52_21735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HLZ89897.1"
                     /translation="MIDEKALFRLVTEGDKEAFRQLFHHYNKMLQPFVVRLTRSPHAA
                     EEVLQEVFLKIWLHREKLAATENPKAYIIRIVSNESVNYLRAQARNGKLLAALQPLAG
                     EEAPSPEESLFYREMETQLNEAIERLPLACRKIYRMSREQYMRIPEIAHALNLSDSTV
                     KNQLVKALKNLRRVVLFPDPDHRIFPIPGTAILLNPALKQNPG"
BASE COUNT          879 a          832 c         1172 g          796 t
ORIGIN      
        1 cgaacaggca caataacctg gctaaatcgg ttggaagcaa aaaattaggg gagaggaggt
       61 catagaccct gccggatggc aggcgatcgg acagtgcaag aggggcaatt aataacggtc
      121 agggcaggga gaccggggct acggaggggt ttgttacaac aacgggctat tgctgccttt
      181 gtgcaatctg cagaacgggt actgcctggc aaggacattc aggcgattca gcgtctcttc
      241 atcgggaggg ggcagcaggg cagcctccgg ttcctttttt gagcggggat gtctgacaat
      301 cttggcggtt ggcaatacgg tcagcggcga gctggcggtt ggagttgtaa cggagacgac
      361 attgggcttc atctggtgag gtgttaacgt tgatgataaa attggtttat aacacacttc
      421 ctatgcaagc tattcttcag tagggaaaca gactggtcgg gggatgagaa ggcaagaaat
      481 cgtgggaagt aatcgctgac tcgaagatac ggagaaaagc ggtggattcg caatgaaaac
      541 gattcttttt tctaaagaaa aataaaattt tcaggtatac gggtgcgtaa atccgacgca
      601 aagatgcatg gactcgttca actgcgcaaa tgcagatggg gcgctggttt cgagacgatg
      661 agtaaaacgg tttagcagga tgcatgtgtt gtctgagcgt tgtcgggatc atcgatgagc
      721 gggtggtgcg ggtgttatcg gtgttgtcct gaaggcgtaa tgataatgga aaaaatgcgc
      781 ggccggcagg acccgggacc aggcagtatc gtcgatctgc aattgggtgc tggtgggata
      841 tagttggcgt aacctgcttt cgatgggtgg tgtggtggcg gttatatggc cgagcaggtt
      901 gctgtcggct gtgagttgga atcctctgcc ggcgcgatcg gtgagggtcc accaacggac
      961 gaggagggca atcggcgccg cggggttttg tccgaaggtc gcgggcagat accggtgatg
     1021 aagggtgggc aggagggtgt cgggcaggtc ttccggggcg atgccatacc aggcgaggga
     1081 gtcggcgccg ggggggagga cccagtccat gccgaagcgc gcgatagtat gcgggaggga
     1141 atcctgccgg gtggtgtcgg tctccagcgt ttgctcgacg agcatggcgc ccgcggcgtt
     1201 gacggtatag ctgaggtgga ggaggcagga gatcccgggc agggtgtatt ccgtccggac
     1261 gatgaccatc tggctcccgg tgctggtgga gaagagctgg aaatggggcg gctcctgcaa
     1321 ggccggtcga agcccgttcg tatcggccag gaaggggata tggttcactt catattgttg
     1381 gatccagccc gtttgtttgt cgaaggagag ccggagggtg ggggagctga cggtgaagac
     1441 gtcgttgctg tcggtgaagc tgagatcgcc ggcggcgggg atggcgatat cgccgcccca
     1501 ggtcttccat ttcaattgct gggtggcgat cggtggcccg ggttgcggcg gccggcggaa
     1561 gccgaccttt aggaaggcct cttccccgcc ggtgaggggg cggagaggga gccggatggt
     1621 cctgggctga ccgggaagca gggagatccc ggggacggtt cctttttgtt tggggatgcc
     1681 gttgacgagg agctgccatt cgagtgttat atttgtataa ttcctgcgga gggagtcgtt
     1741 ggtaacgcga agggcgccct gcatcggaag gaggcttgcg cggatggcct gggcctggga
     1801 aagaatgggg atcccgcaga aaagcgacag ggcgtagggc agtagtttct tcatacgcgg
     1861 gagaggttat aggggggaag attgtaataa taaagaaaaa aagagagaaa tcatgggcta
     1921 aaacgattta gttcatatct ttatagtcca atagcccaaa atgaagaaag tctctatcag
     1981 cgacatcgcc cgcaaagcag gagtgtcggt ctccacggtc tcctttgtca tgaatgacaa
     2041 ggcggtgaaa atgcgcataa gccgcgaggt catcgagaag gtggagaatg tcgccagaga
     2101 gatgggttat cgtcccaacc agcttgcacg cggattgcgg acaggcaaga ccaagaccat
     2161 cgggcttatc gtagaaaata tctccaacgc ctttttcgcc accctcgcaa aatccatcga
     2221 agacgaagca aagcgatacg attacaaagt agtttactgc agtacggata acgatgaaga
     2281 gaaagcgcgc gaactgatca atatgttatc ccagcggcag gtggatggat atatcatcac
     2341 accgacgctg aacctggccg acgacatccg caaactacag gcggagaata aaccggtgat
     2401 cctcatcgac cgttattttc cgcagcatga agagatcccc tcggtactgg tcgataatta
     2461 tgaaggcgtg tcacgcggca tggaaacact gatcgcgaag ggataccgta agatagcgct
     2521 tgtttccatc gaaacggaaa tggcgcatat gaaggaccgg cttcgcgcct actgcgatgt
     2581 cctgaaacgt catggcattt cggaagagca ccggctggtg aagatcatcc cctataacag
     2641 cgaccatgaa cgggcattgg cggaggtgga aagcctttta tcgggcgccg gctccgatat
     2701 cgacgcggtt ttctttctca cgaactacct cggcgtgctc gggatcgaag tgataaagaa
     2761 attgcagata aaggtccccg aacaattggc ggtgatctgt tttgacgata acgatatctt
     2821 ccggttatat accccaacca tctccgtgat ccgtcagccg atcgaagaga tcgggaagag
     2881 ggccatgttt gcgcttgtcg aaagacttcg acattcgggt gaggagtcct ttccggtgaa
     2941 catacctgtc cgcctgacag ccgatcttgt tacgcgtgaa tccatttagc gggatccagt
     3001 cgccgttatc tttatattaa ctgtagggca acggcaggag atgcctgttt tttgttttta
     3061 cttttggcct catgattgac gaaaaagcgc tcttccggtt ggtgaccgaa ggagataaag
     3121 aggctttccg gcagttgttc catcactata ataaaatgct gcaacctttt gtcgtccggt
     3181 tgacgaggtc tccacatgcg gcggaagaag tattacagga ggtttttctg aagatctggc
     3241 tgcaccggga gaaactggcg gcgacggaga atcccaaggc ctatatcatc cggatcgtga
     3301 gcaacgaatc ggtgaactat ctacgcgcgc aggcgaggaa cggcaaactg ttggccgcct
     3361 tacagcccct tgccggcgag gaagcgcctt cccctgaaga atcccttttt taccgggaga
     3421 tggaaacgca actgaacgaa gccatcgagc gactgcccct tgcctgccgg aagatctacc
     3481 ggatgagcag ggaacagtat atgcggatcc cggagatagc ccatgcccta aacctgtccg
     3541 acagtacggt aaagaaccaa ctcgtcaagg ccttgaaaaa tttgcgccgc gtagtcctgt
     3601 tcccggaccc ggatcaccgt attttcccga taccgggtac ggcgatatta ctaaatccgg
     3661 cgttaaaaca gaatccggg
//
LOCUS       DATGFG010001145         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_413959, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001145 DATGFG010000000
VERSION     DATGFG010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413959"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VKQ52_21740"
     CDS             <1..372
                     /locus_tag="VKQ52_21740"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLZ89898.1"
                     /translation="VLGTARAIEYCLAIGEERIEQRVKLLADRLRGQLAAINGVRVLD
                     KGPKLGALVTFTVAGGHPQELVNSLLQRGINVVPSFREFAVIDFDEKKVEWAIRASPH
                     YYNEEWEVDALVAALKELIPR"
     gene            complement(403..1803)
                     /gene="fumC"
                     /locus_tag="VKQ52_21745"
     CDS             complement(403..1803)
                     /gene="fumC"
                     /locus_tag="VKQ52_21745"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HLZ89899.1"
                     /translation="MEYRIEKDTMGEVKVPVDAYYGAQTQRSVINFPIAQDINKMPKE
                     IIRAFAYLKKAAAIVNLEAGVLPKEKSDLIGKVCDEIHSGSLDAYFPLVVWQTGSGTQ
                     SNMNVNEVVAYRGHVLNGGKLTDKEKVLHPNDDVNKSQSSNDTFPTAMHIAAYKILVE
                     TTIPGIEALRNTLADKSKQYMKVVKIGRTHFMDATPLTVGQELSGYVAQLDHGVRAIK
                     NTLAHLSELALGGTAVGTGINTPPGYSENVARKIAELTGLPFVTAANKFEALAAHDAI
                     VEAHGALKTVAVSLMKIANDIRMLSSGPRSGIGELHIPDNEPGSSIMPGKVNPTQCEA
                     LTMIAAQVLGNDVAINIGGATGHFELNVFKPVMIYNFLHSARLIGDGCNSFNEHCAKG
                     IEPIEANIKKHVDNSLMLVTALNTKIGYYKAAEIAQKAHKEGTTLKEMAVKLGYLTPE
                     QFDEWVRPENMVGKLV"
BASE COUNT          435 a          561 c          623 g          509 t
ORIGIN      
        1 gtcctgggta ccgccagggc tatcgaatac tgcctggcca tcggcgaaga gcggatcgaa
       61 cagcgggtga agctgctggc cgaccggctg cgcgggcaac tggcggccat aaacggtgtg
      121 cgggtgttgg acaagggacc aaagctgggc gccctggtca cctttaccgt ggccggcggc
      181 catccccagg aactggtgaa cagcctcctg cagcgcggga tcaacgtcgt acccagcttc
      241 cgggagttcg ccgtcatcga tttcgatgag aagaaagtgg aatgggcaat ccgggcctca
      301 ccgcactatt ataatgaaga atgggaagta gacgcgctgg tcgcggcact gaaagaacta
      361 atcccccgct gaccatcatc ttttttattc tcagctaagg gatcatacca gcttccccac
      421 catattctcg ggccgtaccc actcatcgaa ctgctccgga gtgaggtacc ccagttttac
      481 ggccatctcc tttaatgtcg ttccctcctt atgcgctttc tgggcgatct cggctgcctt
      541 gtagtaaccg atcttcgtgt tcaacgcggt caccagcatc agcgaattgt cgacatgctt
      601 cttgatattg gcttcgatgg gctcgatgcc ttttgcacag tgttcgttga agctgttaca
      661 gccgtcgccg atgagccggg cgctgtggag gaagttatag atcatgacgg gtttgaagac
      721 gttgagctcg aaatgacccg tggccccgcc gatattgatg gcgacatcat tgcccagtac
      781 ctgcgcggcg atcatcgtca gcgcctcgca ttgcgtgggg ttgaccttgc cgggcatgat
      841 ggaagagccg ggttcgttgt cggggatatg gagctccccg atcccgctgc gggggccgga
      901 ggaaagcatc cggatgtcat tggctatctt catcaggctg acggccactg tcttcagggc
      961 gccatgcgct tcgacaatag cgtcgtgtgc ggccagggcc tcgaatttat tggcggcggt
     1021 gacaaagggc agcccggtga gttcggcgat ctttctcgcc acattctccg aataacccgg
     1081 aggagtgttg atccccgtgc cgacggccgt gccgccaagc gccagttcgg aaagatgggc
     1141 aagcgtattc tttatcgccc ttacgccatg gtccaattga gcgacataac cgctgagctc
     1201 ctggccgacg gtgagcggag tggcatccat aaagtgggta cgcccgatct ttaccacctt
     1261 catatactgc ttgctcttgt cggcaagggt attgcgcagg gcctcgatac cggggatcgt
     1321 ggtctccaca aggatcttat aggcggcgat atgcatggcc gtgggaaagg tgtcgttgga
     1381 agactgggac ttgttgacat catcattggg atgcaatacc ttttctttat cggtgagctt
     1441 cccgccgttg agtacatggc cgcgataagc aaccacctcg ttgacgttca tattgctttg
     1501 ggtcccgctc ccggtctgcc agacgacaag ggggaaatag gcgtcgagac tgccgctgtg
     1561 gatctcgtca cagaccttgc cgataagatc ggatttttcc ttgggaagga cacccgcctc
     1621 gagattgacg atggcggcgg cctttttcag gtaggcaaag gcgcggatga tctcctttgg
     1681 cattttattg atgtcctggg cgatggggaa gtttatcaca ctacgttggg tttgagcgcc
     1741 gtagtaggca tcgacgggaa ccttgacctc gcccatcgtg tctttttcga tcctatattc
     1801 cataatactg ttttttgcgg ccccaaagtt aggggattga taccagcgaa tggccggttg
     1861 ggagggtaga aggggaaatt tacgaacggt ttgggcggaa tgatgaaaat ttgcttgttt
     1921 tgttgcgacc caggatcatg ggtccccaaa gctttatata tgacattaac cagattcttc
     1981 tttttattgc tggtcgccgg agccctgtct gccccgtcgg tcctacacgc acaagacgcc
     2041 gatgccaaag acaaatatgt atggaaagaa gcaagctcgg ggggctatac gtataagtat
     2101 gtgaccaacg acccgctgca gacccggc
//
LOCUS       DATGFG010001146         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_910487, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001146 DATGFG010000000
VERSION     DATGFG010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910487"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VKQ52_21750"
     CDS             <1..692
                     /locus_tag="VKQ52_21750"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ89900.1"
                     /translation="IYKGQSDESIFEIAQNSIAESSIIPTNISGVTLASPYIANVSVP
                     AWQLNSATMQTLYYDTSDLRYKQAFVNATGSATPYYECIKYSTIENVNSNTAYQVSLN
                     NILVFRLADIELLKAEALSAQPAPNYGAALTIVNTIRARAGLSTPLTGIAGPALLDTI
                     TAERGRELFLEGHRFYDLVRQARNTGHIQFPAINLAELNAGKNYWPVDPNLFLTNMNL
                     TQTPFWQGRMQ"
     gene            714..1373
                     /locus_tag="VKQ52_21755"
     CDS             714..1373
                     /locus_tag="VKQ52_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89901.1"
                     /translation="MKKFNKYIFFAAIGWMALSTLPSCKKAAYLKDGGISKAKSNLST
                     YDYLKGNEYHYFDTVIALIDHFNLKDSVNKAGTFFAFTDFSVNTLMTNVGATSLEEFY
                     QHVSSQLLTQYMFSDTALTLNNATLAPVQVPNWAGNAALSAIDKIQLAYTVYLTNSAP
                     GFDYYTLQYVQINGVLDGSANAPANDPPDTYISCQTTGIQTATGTTLHVLVNNAPLKI
                     L"
     gene            1384..2361
                     /locus_tag="VKQ52_21760"
     CDS             1384..2361
                     /locus_tag="VKQ52_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89902.1"
                     /translation="MLSNKYIKTLVLLGTVFAGGFNCQKIQTGFLSSNLYYLQNPLIA
                     PQGSITVSSVLQVDGSTAPMNVVMTKVVDSNGNRVDSLMAKKDSFPGFSGAVSYLDSN
                     LALLSKKIVFTSAPALSVNPLGGRIQLTPATNNIPKGMYTIDIKASNVRGAVSIPNAC
                     KIIITSAGSPDTIYSGAYAGTLDNTTGAYAGALGNPSINVQFIPSAVNKIVFKWVDMN
                     GKVYNAAAGGISARTGRWNFRNFDPYYPQVLTDTSVEYQYPTVPNEFPVFPNTGADGS
                     IPRGNFGCFFKIPAAANSTGQGIFTFSDIAFFSQGLYIVTISLSDIAFN"
     gene            2368..>2661
                     /locus_tag="VKQ52_21765"
     CDS             2368..>2661
                     /locus_tag="VKQ52_21765"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HLZ89903.1"
                     /translation="MMRSLFVLLLPALLCAGAGHAQSYVPEWGNKEMKVAPRVAVQAY
                     PFSIKDVRLLDGPFKEAMKADARYLQEIDPDRLLSEFRRHSGLEPKGEKYGGWE"
BASE COUNT          676 a          730 c          643 g          612 t
ORIGIN      
        1 ctatttataa aggacaatcg gacgaaagca tctttgagat tgcgcagaac agcattgccg
       61 agagcagtat catcccgacc aatatatccg gcgttaccct ggcgtcgccc tatatcgcga
      121 acgtaagcgt gcccgcctgg caactgaaca gcgccaccat gcaaacctta tactacgata
      181 cctccgatct ccggtacaag caggcttttg tcaatgctac cgggtccgcc accccctatt
      241 acgagtgcat taaatactcg actattgaga atgtcaactc caacacggcg taccaggtat
      301 cgctgaataa tatccttgtg ttccggctgg cggatataga attgctgaaa gcggaagcct
      361 tatccgccca accggcgccc aattacggag cagccttgac tatcgtgaat accatccggg
      421 cgcgggcggg actttctacc cccctgacag ggatcgcggg ccctgcttta ttggatacca
      481 ttacggccga acggggaagg gaattgttcc tggaaggaca ccgcttctat gatctggtaa
      541 gacaggcgag gaatacgggg catatacaat tcccggctat caacctggca gagctgaatg
      601 ccggtaagaa ttattggccg gtggacccca atcttttttt gaccaatatg aacctgacac
      661 aaaccccatt ctggcagggt agaatgcaat aactaatttt taaatagtac aaaatgaaaa
      721 agttcaataa atatatcttc ttcgcggcca tcggctggat ggcgctatcc accctgccct
      781 cctgtaaaaa ggcagcctac ctgaaggacg gaggcatcag caaagccaaa agcaacctgt
      841 ctacctacga ttacctgaaa gggaacgagt atcattattt cgataccgtt atcgcgctga
      901 tcgatcattt caacctgaag gattcggtga ataaggcggg gacctttttt gcgtttacgg
      961 atttcagcgt gaataccctg atgaccaatg taggagccac cagcctggaa gaattctatc
     1021 agcatgtgag ttcccaactg ttgacgcaat atatgttttc cgacaccgcc ctcacgctga
     1081 ataatgccac cctcgcgccg gtgcaggtac cgaactgggc cgggaatgcc gccctttccg
     1141 ccatcgataa gatacagttg gcgtatacgg tctatctgac gaactcggct ccgggattcg
     1201 actattacac attgcagtat gttcagatca acggggtatt ggatggctcg gccaacgcgc
     1261 cggcaaacga tccgccggat acctatatat cctgccagac aacgggcatc caaacggcta
     1321 ccgggacaac cctgcatgtg ttggtgaata acgccccatt aaaaatactc taaaacagca
     1381 aagatgttaa gcaataaata tataaagacc ctggtcctcc tcgggaccgt cttcgccggc
     1441 ggctttaact gccaaaagat acagacgggt ttcctgagct ctaacctgta ctatctccaa
     1501 aatccgctga tagctcccca gggctccatc acggttagct ccgtgctcca ggtagatggc
     1561 tccacggcgc ccatgaatgt cgtcatgacg aaagtggtgg attcaaatgg caacagggtg
     1621 gatagcctga tggccaaaaa agacagcttt cccggctttt ccggagccgt cagttatctt
     1681 gactccaacc tggccctgct gagcaaaaaa attgtcttta cgtcggcccc cgcgctgtcg
     1741 gtcaaccccc tcggcggccg catacagctg acccctgcca cgaacaatat accaaaaggc
     1801 atgtatacga tcgatataaa ggcttccaat gtcagggggg ccgtctccat accgaatgcc
     1861 tgtaagatca tcatcacttc cgccggttcc cccgacacca tctattccgg cgcttatgcg
     1921 ggaacattgg acaatacgac gggtgcctat gcgggcgccc ttggcaatcc ttccatcaat
     1981 gtgcaattca tcccgtcggc tgtcaataag atcgttttta aatgggtgga catgaatggg
     2041 aaggtctata acgccgcagc aggtggcatc agcgcccgga ccggaagatg gaacttccgg
     2101 aatttcgatc cctactatcc gcaggtgctc acggacacct cggtcgaata ccaatatccg
     2161 acggtgccga atgaatttcc cgttttcccg aatacaggcg ccgacggcag tatccccagg
     2221 ggaaattttg gctgcttctt caagataccg gcggcggcca atagcaccgg acaggggatt
     2281 tttaccttct cggatattgc tttcttctcg cagggactat atattgtaac catcagcttg
     2341 tcggatatag cgtttaatta actttgtatg atgagatctc tttttgtttt attattgccg
     2401 gcactgcttt gcgccggagc gggccatgca cagtcatatg ttcctgaatg gggtaacaaa
     2461 gaaatgaaag tggccccccg ggttgccgtg caggcttacc catttagtat aaaagacgtc
     2521 cgcctcctcg acggcccctt caaagaagcc atgaaggcgg acgctcgcta cctgcaggaa
     2581 atagaccccg atcgcctcct gtcggaattc cgtcgtcact ccggcctcga accaaaagga
     2641 gagaagtacg gcggctggga a
//
LOCUS       DATGFG010001147         6771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1504829, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001147 DATGFG010000000
VERSION     DATGFG010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504829"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VKQ52_21770"
     CDS             complement(<1..910)
                     /locus_tag="VKQ52_21770"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HLZ89904.1"
                     /translation="MTILHTDIYRFSIPMHPFTIATGTMHYAQNILIRIHTDAGYYGV
                     GECSAFPMIVGETQATCFEMAKDFARLWKGKDALQIETRMQDLHDFTAFNATIKSAFD
                     IALHDLAAKAAEQPLYRFLGGKKKAIETDLTIGIDTPENMAARAIQFKADGVRMIKIK
                     LGKKGSEDVERVRRIRAAVGEDIILRIDANQGWDFDTARTTLQQIGAFGVQFCEQPMR
                     HWDDHLLPELRRHSPVKIMADESVFDHHDARRLITAQACDYVNIKFAKSGGILEATKI
                     NRLCEEQNIPCMMGGMLESRVALTAFA"
     gene            complement(1007..2680)
                     /locus_tag="VKQ52_21775"
     CDS             complement(1007..2680)
                     /locus_tag="VKQ52_21775"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HLZ89905.1"
                     /translation="MRKHSPLWPLLCLFLSCHPAPHYDTIIRQGTIYDGSGNKPFTGD
                     IAIQGDTIAAIGDLSGAVATNTIDAKNKAVSPGFIDLQSQSMESLIQDGRSLGAIKQG
                     VTLEIFGEGFSEGPFTDTMKKIEQQQEGDIKYNVEWTTLKDYLEYLQKRGVSTNVASF
                     VGAATIRENFLGYANRAPDSSEQRRMNDLVRKAMEDGALGIGSALIYAPGVYAATPEL
                     IDLCKTAAPYGGVYISHIRSEGNRLLEAADELIRISRDAHIPAIFYHLKAAGRPNWHK
                     LDSLTAKIDSARKTGLDITACMYTYTAGATGFDAAMPPAVQEGGLDKWISRLKDPVIR
                     KKVIREMSTPTKDWENLYLGAGADNIICTGFKQDSLKYLTGKRLSEIAKMRHTTPEET
                     IIDLVIADHSRVEVIYFLMSEENVKKEISLPYMAFGSDEASMAPEGVFLRSSCHPRAY
                     GNFARLLGHYVRDEKLIPLEEAIHKLSGMPAGWLHLKKRGLLKTGNFADVVVFDPAAI
                     KDNSTYEKPHQLATGVQEVFVNGTQVLKAGTHTGAKPGRAVFGPGYKAG"
     gene            2739..3971
                     /locus_tag="VKQ52_21780"
     CDS             2739..3971
                     /locus_tag="VKQ52_21780"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HLZ89906.1"
                     /translation="MSIPPRAVSFENTANAFEYKTNKELKKAEFLFRSMGFSTLVKIG
                     TRVTPWMIRSGFPVRSIIRDTIFSQFVGGETLEETASVARKLGEYRVEVILDYGVEGG
                     EDGEKGFDHACKEFIRVIDYAATQPNIPFMSIKVTGFARFGLLEKLDASMTGPGGPLM
                     RRYAQALESLTQEERDEWQLVSRRMQLICRAAADRKIGVLVDAEETWIQDPVDVLTIL
                     MMEQFNRGRTVVYNTMQLYRTDRLQFLRDSFEAAEQRDFILGAKLVRGAYMEKERRRA
                     LERGVASPIQPDKESTDRDYNAGVEFCIGHLDRIAVIVASHNEHSNLLAVRLLQERGL
                     SLNHRHIHFSQLYGMSDNITFNLAKAGCAVSKYLPFGPIRDVIPYLMRRAQENSSVSG
                     QTGRELGLIRKELRRRGL"
     gene            4109..4182
                     /locus_tag="VKQ52_21785"
     tRNA            4109..4182
                     /locus_tag="VKQ52_21785"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4143..4145,aa:Val,seq:gac)
     gene            complement(4453..4899)
                     /locus_tag="VKQ52_21790"
     CDS             complement(4453..4899)
                     /locus_tag="VKQ52_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89907.1"
                     /translation="MEYPNTQDVLTLPPDYKLYKDRDISIATFLGGPPIAGYLAAENF
                     KRLGETRKARVAWIIAIVVTILIFASVFLIPGIERIPSYLIPLIYSTLARFLVQKYQG
                     AELKAHAFAGGQFYNVWRAVLISLIGLAVLLGLLFALIYLADTPPA"
     gene            5174..5833
                     /locus_tag="VKQ52_21795"
     CDS             5174..5833
                     /locus_tag="VKQ52_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89908.1"
                     /translation="MKRSSVFILIAVVSVLSVACHKNSSGPSAGYGPNSLFPLTAGDT
                     WYYRDSAFNDSGSMETYLDTMVATKATFTDPNSGTIFLGLQDGQRGWFDGAYIAVDPS
                     NYTVYEADSPAFQPYVFFQTVSQDGSIGTGGDYSNPACPISSVQYGYASALTVYGYQC
                     YKNAEVITDCHQNILEEVISYLSPGVGPVRMEDYRTDTTGGKNIFYEDFSATLVGKSL
                     H"
     gene            complement(5876..>6771)
                     /locus_tag="VKQ52_21800"
     CDS             complement(5876..>6771)
                     /locus_tag="VKQ52_21800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89909.1"
                     /translation="TFTQKNQILIPLQGNIWSKGNRYNFINDWRYELYPQDTYGLGGL
                     TKESDGYGIDFSYLRFYQTLLKTIAPDFYLGVGYNVDYFYKVREVDPPAGRVTDFEKY
                     GISPTAVSSGPTLNILYDGRRNAINPEPGYYASLVYRPNFTFLGSDANWQSLLVDARA
                     YKQFPASSKNIIAFWTFDMFTLKGNPPYLQLPATASDTYSNMGRGYIQGRFRGRDLVY
                     LESEYRFGILSNGLLGGVVFVNAQSFTEPGNNRFQTINPGWGAGIRVKLNKFSRTNVA
                     LDYGFGLNGSRGIFANLGEVF"
BASE COUNT         1387 a         1554 c         2199 g         1631 t
ORIGIN      
        1 gggcaaaggc ggtgagggcc acgcggcttt ccagcatgcc gcccatcata caggggatgt
       61 tctgttcttc gcagaggcgg ttgatctttg ttgcttcgag tatgccgcct gattttgcga
      121 atttaatatt gacatagtcg caggcctggg cggtgatgag gcgccgggcg tcgtggtggt
      181 cgaacacgct ttcgtcggcc atgatcttta ccggcgaatg ccggcgcagt tcgggcagca
      241 ggtgatcgtc ccagtgccgc atcggttgct cacagaactg gacaccgaag gcgccgatct
      301 gctgcagcgt ggtgcgggcg gtgtcgaagt cccagccctg gttggcgtcg atccgcagga
      361 tgatgtcttc gccgacggcg gcgcggatcc ggcggacgcg ttctacgtct tcggaacctt
      421 tcttacccag ttttattttg atcatccgga cgccgtccgc cttgaactga atggctcttg
      481 ccgccatatt ctccggggtg tcgatgccga tggtgaggtc ggtctcgatg gcctttttct
      541 ttcccccgag gaagcggtag aggggttgtt ccgccgcctt tgcggcaaga tcgtgcaatg
      601 ctatgtcgaa tgcgcttttg atggtcgcgt tgaaggcggt gaaatcgtgg agatcctgca
      661 tccgggtctc tatctgcagg gcgtccttac ctttccacag gcgtgcgaag tcttttgcca
      721 tctcgaagca ggtggcctgg gtctcgccga cgatcatcgg gaaggctgag cattcgccga
      781 cgccatagta gccggcatcg gtgtgtatgc ggatcaggat gttctgggca tagtgcatcg
      841 tacctgttgc aatggtgaaa ggatgcatgg ggatggaaaa cctgtagatg tcggtatgga
      901 ggatggtcat agttgtaata tggttggcgc aagtttgccg gttagcgcgg caaagatagg
      961 ggcttgcgcg ttatgatggg tgtggcgcaa tgcttgcggg atcgggtcag ccggccttat
     1021 atccgggacc gaagacggcc cggccgggtt ttgcgccggt gtgggtaccc gctttcagca
     1081 cctgtgtccc gttgacgaac acctcttgca cgccggtggc cagctggtgg ggtttttcgt
     1141 aagtggaatt gtctttgatg gcggcggggt cgaagaccac gacatcggca aagttgcctg
     1201 ttttgagcag gcctcttttc ttcaggtgta gccagccggc gggcataccg ctgagtttat
     1261 ggatggcttc ttccaggggg atcagctttt cgtcgcggac atagtgaccc agcaggcggg
     1321 cgaagttgcc ataggcgcgg gggtggcaac tgcttcgcag aaatacgcct tcgggcgcca
     1381 tgctggcttc gtcggacccg aaagccatat aggggagact tatctctttc tttacgttct
     1441 cctcactcat gagaaagtag atcacttcga cacgggaatg gtcggcgata acgagatcga
     1501 tgatggtctc ttcgggggtg gtatgccgca tcttggcgat ttcgctcaat cttttgccgg
     1561 tgaggtattt cagggagtcc tgtttgaagc cggtgcaaat gatgttatcg gcgccggcgc
     1621 ccaggtagag gttttcccaa tcctttgtcg gcgtactcat ctccctgatc acctttttgc
     1681 ggatgacggg gtctttgagg cggctgatcc atttgtccag cccgccttcc tgcactgcgg
     1741 gcggcattgc ggcgtcgaag ccggtggccc cggcggtgta ggtgtacata caggcggtga
     1801 tgtcgaggcc cgttttgcga gcgctgtcga tctttgcggt gagactgtcc agtttgtgcc
     1861 agttgggacg gccggcggct ttgaggtggt agaagatggc ggggatatgg gcgtcgcggg
     1921 agatgcggat cagctcatcg gcggcttcca ggaggcggtt gccttcgctg cggatgtggg
     1981 aaatatagac accgccatag ggcgccgcgg tcttgcataa gtcaatgagt tcgggtgtcg
     2041 cggcatagac cccgggggcg tagatgagcg cggagccgat gccaagggcg ccgtcttcca
     2101 ttgcttttcg gacgagatcg ttcatcctgc gttgttcgga gctatcgggg gcgcggttgg
     2161 catagccaag gaaattctcc cttatcgttg ccgcgccgac gaaggatgcg acgtttgtcg
     2221 atacgcccct tttctgtagg tattcgagat agtctttcag cgtggtccat tcgacgttgt
     2281 acttgatatc gccttcctgt tgctgctcga tctttttcat ggtgtcggtg aatggtcctt
     2341 cggagaatcc ttcaccgaag atctcgaggg tgacgccctg ctttatggcg ccaagggagc
     2401 ggccgtcctg gatgagggat tccatggact ggctctggag atcgatgaat cccgggctga
     2461 cggctttgtt ctttgcgtcg atggtgttgg tggcgactgc cccggaaagg tcaccgatcg
     2521 cggcgatggt atcgccctgg atggctatat cgccggtgaa aggtttgttg ccgctgccat
     2581 catagatggt tccctggcga atgatggtgt cgtaatgagg agcgggatgg caggaaagga
     2641 aaaggcagag cagcggccag agcggcgagt gctttctcat aagcgacaat ttaatcccaa
     2701 aatacggtat ttttgcggcc aaattaccgt ctgtaagtat gagtatcccc ccgagagctg
     2761 tttcttttga aaataccgcc aatgcttttg agtacaagac gaacaaggaa ctgaagaaag
     2821 cggaattcct tttccggagt atgggttttt ccacgctggt gaagatcgga acaagggtta
     2881 cgccctggat gatccgttcg ggttttccgg tgaggagtat catccgggat acgatctttt
     2941 cccagtttgt cgggggagag acgctggagg aaacggcttc tgttgcgcgg aagctgggtg
     3001 aatacagggt cgaggtgatc ctcgattatg gggtggaagg gggagaagac ggtgaaaagg
     3061 gatttgatca tgcgtgtaag gagtttatcc gggtgatcga ctatgccgcc acccagccca
     3121 atattccctt tatgagcatc aaggtgacgg gttttgcgcg gtttggcctg ctggagaaac
     3181 tggatgcgtc gatgaccggt cccggtgggc cgctgatgag acggtatgcg caggcgttgg
     3241 agtcgctgac gcaggaagaa cgggatgaat ggcagctggt gagcaggagg atgcaactga
     3301 tctgccgggc ggcggctgac cggaagatcg gcgtgctggt ggatgcggag gagacctgga
     3361 tacaggaccc ggtggatgtg ctgacgatcc tgatgatgga gcagttcaac aggggccgga
     3421 cggtggtgta taatacgatg cagttatatc gtaccgacag attgcaattc ttaagagatt
     3481 ccttcgaggc ggcggagcag cgggatttta tcctgggggc gaaactggta cggggcgcgt
     3541 atatggaaaa ggaacggagg cgggccctgg agaggggagt ggcgtcgccg atccagccgg
     3601 ataaggaatc gaccgaccgg gactacaatg ccggggtgga gttttgtatc gggcacctgg
     3661 accgcatcgc cgtgatcgtc gcttcgcata atgagcacag caatctcctt gcggtgaggt
     3721 tgttgcagga gcgggggttg tcattgaacc accggcatat tcatttttca cagttgtatg
     3781 ggatgagcga caatatcacc ttcaacctgg cgaaggccgg ttgtgcggtg agcaagtatc
     3841 tgccttttgg gccgatcagg gatgtgatcc cttatcttat gcgcagggcg caggagaaca
     3901 gctcggtctc gggccagacg gggcgggagc ttgggcttat tcgaaaagaa ctgaggcgta
     3961 gggggttgta atcgacgctg tgttcgatct cgttgtagtt ctccataaaa aaatcgggag
     4021 tatttgggtg gtggttttgg taaatagggg aatttaatta aaatttgcac tctgaattgc
     4081 ggctgatcga tagattggct ggttataagg gtcgttagct cagttggtta gagtactacc
     4141 ttgacatggt aggggtcacc ggttcgaatc ctgtacgacc cacaaggccc cgcggtgata
     4201 gccgcggggc ttttttcatg cggtagcgag gcgaatgaat tcgccggagc ggatgcgtga
     4261 aaaaagtcca tcccgcgaga gcgggataag gggccttgag taagggaacc gccggaggcc
     4321 agggagcagc gaagctaatc ctgtacgacc cacgaggccg cccagacgat agtctgggcg
     4381 gctttaatat ggcgagcgga tcaggtatat ttcgggcgga gaactcaaaa aggcggctgg
     4441 ccgctggttt gtttaagcag gaggggtgtc cgcgagatag atgagcgcga agaggagtcc
     4501 gagcaagacg gcgaggccga taaggcttat cagtacggcg cgccagacgt tgtagaattg
     4561 gcctccggcg aaggcatgcg ctttgagctc cgcgccctgg tatttttgta cgaggaatct
     4621 tgcgagcgtg gagtagatca gggggatgag gtaggagggg atgcgttcga taccggggat
     4681 gaggaagacg ctggcgaaaa taagaatggt gacaacgatg gcaatgatcc aggctactct
     4741 tgctttgcgt gtttcgccga ggcgtttgaa attctcggcc gccaggtagc cggcgatggg
     4801 tggtccgccg agaaaggtgg cgatgctgat atccctgtcc ttgtatagtt tgtagtccgg
     4861 tggaagggtg agtacgtcct gggtattggg gtattccata agtttggcgg ttgagatagc
     4921 caaaataata ctattcgacg gttatgaaaa aaaattgagg ggagggggaa aaagaaatga
     4981 gggagggggg aaggattaaa attgccttaa aacacaacat tggcggcaga cttttcgtat
     5041 agtctttagg atttcgttaa caaatcccgg ataaacttta ccattgtccg ggaatcgaac
     5101 cagccgaaat cccttgttat gacacgaggc gggagccgac tgcataacgg tgttgaaaaa
     5161 taatcgattc gttatgaaaa gatcttctgt atttatcctg atcgccgtcg tatccgtact
     5221 ttccgttgcc tgtcacaaga attcatccgg tccgagtgcg ggatacggcc ccaattccct
     5281 tttcccgctg accgcggggg acacctggta ttaccgggat tcagccttca atgattcggg
     5341 ctccatggag acctatctgg acacgatggt agccacgaag gccacgttta ccgatccgaa
     5401 ctcggggact atttttcttg gtttgcagga tggtcaaaga ggttggttcg atggcgccta
     5461 tattgcggtc gatccttcca attacaccgt ctatgaagcc gacagcccgg cgtttcagcc
     5521 ttatgttttt ttccagaccg tcagccagga tgggtcgata ggtacgggag gggattattc
     5581 caatcctgcc tgcccgatca gttccgttca gtatggctat gccagcgcgc ttacggtata
     5641 cggctatcaa tgttataaga atgccgaagt gatcaccgac tgtcaccaga acatactgga
     5701 ggaagtgatc tcctacctga gtcccggagt ggggcctgtg cgaatggagg actatcgaac
     5761 ggacacgacg ggtgggaaga atatcttcta tgaagatttt tccgcgacgt tggtcggaaa
     5821 gagtttgcat tagctgatga ccggccacag atcggccggc cgatctgtgg ccggcctaga
     5881 atacctcgcc gagattggcg aagatgccgc gcgagccgtt caggccaaag ccatagtcga
     5941 gcgcgacgtt cgttctcgag aatttattga gtttgacgcg gatgccggcg ccccagccgg
     6001 ggttgatggt ttggaaccgg ttgttgccgg gctccgtaaa ggattgtgcg ttgacgaaga
     6061 cgacaccgcc gaggaggccg ttcgacagga tgccaaagcg gtattcggat tcgaggtaga
     6121 ccagatccct gccgcggaac cttccctgga tgtatccccg gcccatgttg ctgtaggtat
     6181 cggatgccgt ggcaggaagc tggaggtagg gtggattccc tttcagggtg aacatatcga
     6241 aggtccagaa cgcgatgata ttttttgagc ttgcggggaa ttgcttgtat gcacgggcat
     6301 ctaccagcag gctttgccag ttggcgtcac tgccgaggaa ggtgaaattg ggccggtaga
     6361 caagactggc ataatagccg ggctcggggt taatggcgtt gcgccggcca tcgtagagga
     6421 tgttgagggt agggccggag gagacggcag taggtgaaat gccatatttt tcgaagtcgg
     6481 tgacccggcc ggccggcggg tcgacctctc ttaccttata gaaataatcc acattatagc
     6541 cgaccccgag ataaaagtcg ggggcgatgg tttttaagag cgtttgatag aaccgtaaat
     6601 aggagaagtc gatgccatag ccgtcgcttt cctttgtcaa tccaccgagc ccataggtgt
     6661 cctgcgggta gagctcgtat cgccagtcgt tgataaaatt gtacctgttc cccttgctcc
     6721 agatgtttcc ctgcaggggg atgaggatct ggttcttctg ggtaaaggtg t
//
LOCUS       DATGFG010001148         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_414032, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001148 DATGFG010000000
VERSION     DATGFG010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414032"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VKQ52_21805"
     CDS             <1..187
                     /locus_tag="VKQ52_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89910.1"
                     /translation="LRWQSAGRLFLLLDAAYAGANPDFHYNYSIKVNGNFFTSGEAEK
                     KLPTGTIQLRLGAGLRL"
     gene            complement(191..1246)
                     /locus_tag="VKQ52_21810"
     CDS             complement(191..1246)
                     /locus_tag="VKQ52_21810"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HLZ89911.1"
                     /translation="MKFEVGDKVVIQHSNDEGEVVEIINDKMVLVDVRGVKFPAYTDQ
                     LDFPYFKRFTEKKLVPPEKKPKKYVDQLPPEKPAPGRKKERIAQLADGVWLTFLPVFD
                     ADEFGDDVVESLKIYLVNNTPLGYGFSYHLNYFGKSEFDLDNQLPSGQDFYLHDIPFA
                     NLNDNPVFECEYTLLTPDKSRAEHYETSLKLRAKQVFSRIEEIRKKGEATFSFKLFDS
                     YPFREVEESVDTRGVLSGSGFRVYEASRARQHLEPARSELDLHAERLTDKAASLDAFE
                     ILTLQLQTFEKYFDLAVAHHLPSMIVIHGVGTGKLRDEIHEELRTKRNVKSFVNRYDP
                     RYGYGATEIFFEYGKKK"
     gene            complement(1256..>2129)
                     /locus_tag="VKQ52_21815"
     CDS             complement(1256..>2129)
                     /locus_tag="VKQ52_21815"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLZ89912.1"
                     /translation="LYILIALVLTGMVSYKELRVTDPLAYVFNRVHQDWIAYFISASA
                     VVATTSVLLVFQLGQPRIWMSMSRDGLLPKAFGKIHKKYHTPWFSTIVTGIIVSVPSL
                     FMGTELMQNLTSIGTLFAFVLVSWGVLVLPRNDGIKGQGFKLPYVNGRWILPVAYAAF
                     IVLFRTRLTGIFQELTAKGMHDFFFRPDEKGKIVFSNEALQAALFIVFTLLATAFSVA
                     TVVRKYSVIPVLGVLFCAYLLIEIPAVAWEWFFVWMGIGLLVYFFYGYRKSMLTAGAG
                     RQVGKVAGQSQAKL"
BASE COUNT          510 a          682 c          510 g          427 t
ORIGIN      
        1 cctccgctgg cagtccgcag gcaggctatt cctcttactg gacgcagcat acgcgggcgc
       61 caacccggat tttcactaca actactcgat aaaagtcaat ggaaatttct ttacctcggg
      121 cgaggcggaa aaaaagcttc ccaccggcac catccagctt cgcctgggcg ccggcctacg
      181 cctttgatga ttatttcttc ttcccatact cgaagaagat ctccgtcgct ccgtacccgt
      241 agcgcggatc ataccggttg acaaaagatt tcacattgcg cttcgtccgc agctcctcat
      301 ggatctcatc ccgcagcttc cccgtcccca ccccatggat cacgatcatc gatggcagat
      361 gatgcgcaac ggccagatcg aaatatttct cgaaggtctg caactgcagg gtcaggatct
      421 cgaaagcatc gagcgaagcc gccttgtcgg tcagcctctc cgcatgcaga tccagctcgc
      481 tgcgcgccgg ctcgagatgc tgccgcgccc gcgaagcctc atacacccga aaacccgagc
      541 ccgacaggac accccgcgta tccaccgact cttccacctc ccggaaggga taggaatcga
      601 agagcttgaa ggaaaaggtc gcctccccct tcttcctgat ctcctcgatc cggctgaata
      661 cctgcttggc ccgcagcttt agcgaggtct cataatgctc ggcccggctc ttgtccggcg
      721 tcagcagcgt atactcacac tcgaacacgg gattgtcatt caggttggca aagggaatat
      781 catgcaggta gaaatcctgc cccgaaggga gctggttgtc caggtcgaac tcactcttcc
      841 cgaaataatt caggtgatag gagaatccat accccagcgg cgtattgttg acaagataga
      901 tcttcaggct ctccaccaca tcatccccga actcatcggc atcgaaaaca ggcagaaagg
      961 tcagccagac cccatccgcc aactgcgcga tccgctcctt cttcctgccc ggcgccggct
     1021 tctccggcgg cagctgatcg acatatttct ttggcttctt ctccggcgga acaagcttct
     1081 tttcggtaaa tcgcttaaaa taagggaaat ccaactgatc ggtataagcc gggaatttca
     1141 ccccgcgcac gtctaccagg accatcttgt cattgatgat ctccaccacc tcgccctcat
     1201 cgttggaatg ctgaatgacg accttgtcgc ctacctcgaa cttcataccg caaagttaca
     1261 actttgcctg cgattgaccc gccaccttcc cgacctgccg gccggccccg gccgtcaaca
     1321 tgctcttccg gtacccatag aagaaataga ccagcagccc gatccccatc cagacaaaga
     1381 accattccca ggcaacggcg ggtatctcga tcagcagata ggcgcagaac agcaccccca
     1441 gcaccgggat gacggagtat ttccggacga cggttgccac cgaaaacgcc gtcgccagta
     1501 gggtgaatac gataaaaagc gcggcctgca aagcttcatt ggagaagacg atcttcccct
     1561 tctcatctgg ccggaaaaag aaatcatgca ttcccttcgc ggtcaactcc tggaagatcc
     1621 ccgtaagcct ggtccggaac aacacgataa acgccgcgta ggcaaccggg aggatccagc
     1681 ggccgttgac atagggcagc ttgaaacctt gtcccttgat gccatcgttt cgcggcaaca
     1741 ccagcacccc ccaggacacc agcacaaagg cgaacagcgt cccgatgctg gtcaggttct
     1801 gcatcagctc cgtccccata aagagcgagg gcaccgaaac gatgatcccg gtgacgatcg
     1861 tggagaacca gggcgtatga tactttttgt ggatctttcc aaaggctttg ggcaacaacc
     1921 cgtcccggct catgctcatc cagatccgcg gctgccccaa ttggaagacc agcagcacgc
     1981 tcgtcgtagc caccaccgcg ctggccgaaa tgaaataggc gatccaatcc tgatgcaccc
     2041 ggttgaacac ataggcaagg ggatcggtta ctctcagctc cttatagctg accataccgg
     2101 tcaacacaag cgcaataaga atatagagt
//
LOCUS       DATGFG010001149         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_328321, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001149 DATGFG010000000
VERSION     DATGFG010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328321"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VKQ52_21820"
     CDS             complement(<1..841)
                     /locus_tag="VKQ52_21820"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLZ89913.1"
                     /translation="MSETFRPVDWIYSSNIYEVNLRQYTSEGTLRAFINELPRLRDMG
                     IDVLWFMPITPISREKRLGSLGSYYACSDYISINPEYGTMDDFTALVKEAHTLGFRVI
                     LDIVANHTGWDHHWTHEHPDYYRRNQEGRFFDPHGWEDVIDLNYDNPELRKAMIDVMR
                     FWIEQGDVDGFRCDMAMLVPLDFWKQAREALDPLKPLFWLAECEEIAYHEVFDATYTW
                     KFLHKMESVWRKESPVSGLDEVLDYYDTMFPNDGLRVYFTTNHDENSHSGSEFGRMGD
                     MAKP"
     gene            908..2143
                     /locus_tag="VKQ52_21825"
     CDS             908..2143
                     /locus_tag="VKQ52_21825"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HLZ89914.1"
                     /translation="MKNFDIPIIYRSPLISAIKNKRKQQDKMKKDFTPTLLDLGGIQL
                     YLARHFGFCYGVENAIEISFRTIEENPDRRIFLLSEMIHNPQVNADLQSRGVRFLQDT
                     HGRQLMPFEELTAEDIVIIPAFGTTLEIERELQDIGIRTEKYNTTCPFVEKVWNRSSQ
                     IARKDYTIIVHGKPGHEETRATFSHAAAETPTVVVKDMSEARVLAQYVRGELPAESFY
                     TTFKGQYSTGFDVQRDLVRIGVVNQTTMLASDTQAIADYLKQTMAGQYGLTEADVDTH
                     FADTRDTLCYATLDNQSAVQGLLDVPADLAIVVGGYNSSNTSHLVELCEERMPTYFIS
                     SEEKILSPQEILHYDFHHKTEKRQAGWLPEGRPVRILISSGASCPDALVEAVIRKIAG
                     YFDAAETVESLVESFATPS"
     gene            complement(2130..3467)
                     /gene="accC"
                     /locus_tag="VKQ52_21830"
     CDS             complement(2130..3467)
                     /gene="accC"
                     /locus_tag="VKQ52_21830"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HLZ89915.1"
                     /translation="MFKKILIANRGEIALRVIRTCREMGIKTVAVYSTADKESLHVKF
                     ADEAVCIGKPASFDSYLNIPHIMAAAEITNADAIHPGYGFLAENARFAEICGEHEIKF
                     IGPTPSMINSMGDKITAKETMIKAGVPVVPGGEGLLESLDQAKGMAKEVGYPIILKAT
                     AGGGGKGMRVVWEEADMERAYNTAKAEAGAAFKNDGIYMEKFVEEPRHIEIQVAGDQY
                     GKVCHLSERDCSIQRRHQKLVEESPSPFMTPELRQRMGDAAIKAASAINYESVGTIEF
                     LVDKHRNFYFMEMNTRIQVEHCVTEEVVNFDLIKEQIKIAAGDTISGLNYEPQGHAIE
                     CRINAEDPYNDFRPSPGRINVLHQPGGHGIRVDSHAYAGYTIPPYYDSMIAKIIAVAR
                     TREEAINTMHRALSEYVIEGIKTTIPFHLQLMKDERFRAGDFNTKFLDSFKMV"
     gene            complement(3477..>3947)
                     /gene="accB"
                     /locus_tag="VKQ52_21835"
     CDS             complement(3477..>3947)
                     /gene="accB"
                     /locus_tag="VKQ52_21835"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HLZ89916.1"
                     /translation="QELIKMINKSNIGEVSIEEKGFKLTIKQKEEPVQQVIAAPVHPQ
                     PIAAMAAAPVAAPQPQATQAAEKSAKAAEAPAGNTVTIKSPMIGTFYRSPSPDKPSFI
                     NVGDEITPGKVVCIIEAMKLFNEIESEVSGKIVKVLVEDASPVEYDQPLFLVEP"
BASE COUNT          782 a         1011 c         1188 g          966 t
ORIGIN      
        1 agggtttggc catatcgccc atccgcccga actcgctgcc gctgtgggaa ttctcatcgt
       61 ggttggtggt aaaatagacg cggagcccgt cgtttgggaa catcgtatcg taataatcca
      121 gcacttcgtc gagcccggag acgggtgatt ccttccgcca taccgattcc atcttatgca
      181 ggaatttcca ggtataggtg gcgtcgaata cttcatgata ggcgatctct tcgcattcgg
      241 ccagccagaa gaggggtttg aggggatcga gggcttcgcg cgcctgtttc cagaaatcca
      301 ggggcacgag catggccata tcgcagcgga agccatcgac atcaccctgc tctatccaga
      361 accgcatcac gtcgatcatt gccttgcgca attccgggtt gtcatagttg agatcgatga
      421 cgtcttccca gccgtggggg tcgaagaacc gtccttcctg gttacggcgg taatagtcgg
      481 ggtgttcatg ggtccagtga tggtcccagc ccgtatggtt ggcaacgata tcgaggatga
      541 cccggaaccc gagggtatgc gcttctttta ccagcgcggt aaaatcgtcc atcgtaccat
      601 attcgggatt gatgctgata tagtcggagc aggcgtaata gctgcccagc gagcccagcc
      661 gtttctcccg gctgatgggg gtgatgggca tgaaccaaag aacatcgatg cccatatccc
      721 tcaaccgggg gagttcgttg ataaaggccc ggagggtgcc ttcggatgtg tactggcgca
      781 gattgacttc ataaatattg gatgaataga tccagtctac agggcgaaat gtctcactca
      841 tagctcttta acgcgtggtt tgctactaag gtatgccgaa aaaggctatt ttgtaccttt
      901 gtacgtcatg aagaacttcg atatcccgat catctatcgc agcccgttga tctccgccat
      961 caagaacaag cgcaaacagc aggacaaaat gaaaaaggat tttacgccta ccctgctgga
     1021 tctgggtggg atacagctgt atctggcgcg ccatttcgga ttttgttacg gggtagagaa
     1081 tgcgatcgag atctctttcc ggacgatcga ggagaatccc gaccggcgga tctttctgct
     1141 gagcgagatg atccacaacc cccaggtgaa tgccgatctg caatccaggg gtgtacggtt
     1201 cctgcaggat acccatgggc ggcaattaat gccctttgag gagctcacgg cggaggatat
     1261 cgtcatcatt cccgcctttg gcaccacgct ggagatcgag cgggaattgc aggacatcgg
     1321 catccggacg gaaaaataca ataccacctg cccctttgtg gagaaggtct ggaaccgcag
     1381 cagccagatc gcgcggaagg actataccat catcgttcat gggaagccgg ggcatgagga
     1441 aacgagggcc actttctcgc atgcggcggc ggagacgccg acggtggtgg tgaaggatat
     1501 gagcgaggcc agggtgctgg cccaatatgt caggggggag ttgccggcgg aaagctttta
     1561 caccaccttt aaagggcagt acagcacggg tttcgatgtt caacgcgatc ttgtgcggat
     1621 cggggttgtc aaccagacga cgatgctggc aagcgatacc caggccattg ccgactatct
     1681 gaaacaaacc atggccgggc aatacggcct gacggaggcg gatgtcgata cccattttgc
     1741 ggatacgcgg gacacgctgt gttacgccac gcttgacaat cagtctgccg ttcaggggtt
     1801 gctggatgtg cccgccgacc tggcgatcgt cgtgggtggg tataattcct ccaatacctc
     1861 gcatctcgtg gaattgtgcg aggaacggat gcctacctat tttatctcct cggaggagaa
     1921 gatactttcc ccgcaggaga tcctgcatta tgattttcac cacaagacgg agaagcggca
     1981 agcggggtgg ctgccggaag ggcggcccgt acgtatcctg atcagcagcg gggcctcctg
     2041 cccggacgcg ctggtagagg cggtgatccg gaagatcgcc ggttattttg acgctgcgga
     2101 aaccgtggag tcgctggtgg agagctttgc tacaccatct tgaacgaatc gaggaatttc
     2161 gtgttgaagt cccctgcccg gaagcgttcg tctttcatca attgcagatg gaaggggatg
     2221 gtcgttttga tcccttcgat gacatattcg ctcagcgctc ggtgcatggt attgatcgct
     2281 tcttcccggg tacgggcgac ggcgatgatc ttggcgatca tggagtcgta gtagggcggg
     2341 atggtatagc cggcgtaggc atgcgagtcg acgcggatgc cgtggccgcc gggctggtga
     2401 agcacgttga tgcggccggg tgagggtcgg aaatcgttgt aggggtcttc ggcgttgatc
     2461 ctgcactcga tggcatggcc ctggggctcg tagttgagtc cgctgatggt gtcgccggcg
     2521 gcgatcttga tctgttcttt tatgagatcg aaattgacca cttcttcagt gacgcaatgt
     2581 tctacctgga tacgggtgtt catctccatg aagtagaagt tgcggtgttt gtcgacgagg
     2641 aattctatcg tccccacgct ttcgtagttg atggcggagg cggcctttat agcggcgtcg
     2701 cccatgcgct ggcgcagttc gggcgtcata aagggggagg gggattcctc cacgagtttc
     2761 tggtggcgac gctggataga gcagtcgcgt tcgctgagat ggcagacctt gccgtattgg
     2821 tcgccggcca cctggatctc gatgtgccgg ggttcttcga caaatttctc catgtagatg
     2881 ccgtcgttct tgaacgcggc gcctgcctcg gccttggcgg tgttataggc tcgttccata
     2941 tccgcctctt cccagaccac ccgcatcccc ttgcccccgc cgccggcggt ggctttgagg
     3001 atgatgggat acccgacctc ttttgccatg ccttttgcct ggtcgaggct ttcgagcagg
     3061 ccttcgccgc ccgggacaac gggtacgccg gccttgatca tggtctcttt tgcggtgatc
     3121 ttatccccca tcgaattgat catggaggga gtggggccga tgaattttat ttcgtgttcg
     3181 ccgcagatct cggcgaaacg ggcattttct gcgaggaacc cgtaaccggg atggatggcg
     3241 tccgcgttgg tgatctcggc ggcagccatg atatgtggga tattgagata ggaatcgaag
     3301 ctggcgggct ttccaataca cacggcttca tcagcgaact tgacatgcag gctttcctta
     3361 tccgccgtgg agtagaccgc tacggtcttg atgcccatct cgcgacaggt acgaatgacc
     3421 ctgagggcaa tttctcctcg gttggcgatc agtatctttt taaacatgtt gtgattttag
     3481 ggttcaacca ggaacagggg ctgatcgtac tcaacggggg aggcgtcctc taccagcacc
     3541 tttactattt ttcccgatac ctcgctctcg atctcgttga agagtttcat ggcttcgatg
     3601 atgcagacca cctttccggg tgtgatctcg tcgcccacat ttataaacga gggtttatcc
     3661 ggcgacgggc tacggtagaa cgtgccgatc atgggactct ttatggtaac cgtattgccg
     3721 gccggggctt ccgcggcctt ggcggatttt tccgcggcct gagtggcctg tggttggggg
     3781 gcggctaccg gggccgcagc catggcggct atcggttgag ggtgtacggg ggccgcgatc
     3841 acctgttgca ccggttcttc tttttgcttg atcgtcagtt taaatccttt ctcttcgatg
     3901 cttacttctc cgatatttga tttgttgatc attttgatca actcctg
//
LOCUS       DATGFG010001150         4838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_302889, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001150 DATGFG010000000
VERSION     DATGFG010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302889"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /gene="bcp"
                     /locus_tag="VKQ52_21840"
     CDS             <1..502
                     /gene="bcp"
                     /locus_tag="VKQ52_21840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HLZ89917.1"
                     /translation="FTKKIATMTHLQEGDKAPVFTGKDAAGNKISLADHKGKKVALYF
                     YPQDDTPTCTVQACNLRDNFALLKKEGFSVIGVSPDDEKSHQKFGKKFGLPFPLVADA
                     DHKIIDKYGVWGEKQLYGRKYMGLHRTTFLIDEKGVIRKIFLKPRSKQHAEEIVAAWA
                     AVAPGK"
     gene            complement(597..1301)
                     /locus_tag="VKQ52_21845"
     CDS             complement(597..1301)
                     /locus_tag="VKQ52_21845"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HLZ89918.1"
                     /translation="MDSTNNYAMARATAGAANHGTLVFAHDQWAGKGQRGRVWTSTPG
                     ENIILSAVLEPVALPPTAVFALSVAVALACHDLFSHHAGADHTSIKWPNDLYWNDRKA
                     GGILIENHFKGERWLFAIAGMGININQVEFPSTARNPVSLRQITGKTFDSLQLARELG
                     VFLDKRYSALVAGGAADQLQQYNRRLYGRAREVRLKKDNAVFKTVVQGVSAQGELLTR
                     DVLDRQFSFGEVEWLI"
     gene            1419..1823
                     /gene="rsfS"
                     /locus_tag="VKQ52_21850"
     CDS             1419..1823
                     /gene="rsfS"
                     /locus_tag="VKQ52_21850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HLZ89919.1"
                     /translation="MEQLSILSTRKRSSVVRLTRNSKIFKSIIKAIQEKKGENIISLD
                     LRKIPEAVADFFIICEAASTTQVKAIADSVEVEVKNNCDELPYKHEGRQTYQWVLIDY
                     VNVVVHVMLPENRRFYKLEDMWSDAPQEEHQG"
     gene            1943..4000
                     /gene="ftsH"
                     /locus_tag="VKQ52_21855"
     CDS             1943..4000
                     /gene="ftsH"
                     /locus_tag="VKQ52_21855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HLZ89920.1"
                     /translation="MPQDDLKQNDKNNFPRFRPRGDDDNGQRKGPKFSIYWIWGIIAA
                     VLIGFNLFGSFSPDAHPADELDFRKTMLAQGDVEKIDLISNKNLVRVYIKKDSLNKPF
                     YSKYSKQNSWPGGMSKDGPQFEFRVTDIKDYEKILREYFATHPNQEVAINSKQEGEWF
                     GPLLNFLLPLLIIVLIWVMLMRKMGGQAGTGGPGGIFNIGKSKATLFDKGTKVTITFS
                     DVAGLDEAKVEVMEIVDFLKNPKKYTALGGKIPKGALLVGPPGTGKTLLAKAMAGEAQ
                     VPFFSMSGSDFVELFVGVGASRVRDLFKQAREKAPCVIFIDEIDAIGRARGKNAIMSN
                     DERESTLNQLLVEMDGFGGDSGIIVLAATNRPDVLDTALLRPGRFDRQISIDKPDLKG
                     REAIFKVHLKPIKISSKLDIHKLAEQTPGFAGADIANVCNEAALIAARKGKETVEMED
                     FQDAVDRVIGGLEKKNKIISPDEKRIIAYHEAGHAICGWYLEHAYPLLKVTIVPRGVA
                     ALGYAQYTPKEQYLYNTDQLFDQICMTLGGRASEELNFGKISTGAQNDLQQITKIAYS
                     MVTVYGMNEKVGNVSFYDPQAENTFTKPYSEETSRLIDEEVRKLIDKAYEATKHLLTD
                     KTREVRLLAEALLQREVLFQSDVEALIGKRPYEEKRALDVSEHDNGVPPAVVVPPATE
                     EVK"
     gene            4003..4641
                     /locus_tag="VKQ52_21860"
     CDS             4003..4641
                     /locus_tag="VKQ52_21860"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HLZ89921.1"
                     /translation="MKVSSAKENILKKIRQALSESTPIPFPQSEGSASVFHPSRQDLE
                     IEFAEQFAKLQGKFVFCVDHGELVAQLNALLTSQGLKKLYCREKKLRETLKTHGFDGF
                     SDVELGDCDAAVTTCEGLVARTGSIILSSAGASGRTVSVYAPVHICVAYTDQLVYDIR
                     DGLVGLKEKYQQQLPSLMTFATGPSRTADIEKTLVVGVHGPKQVYLFLVDRQ"
BASE COUNT         1208 a         1270 c         1340 g         1020 t
ORIGIN      
        1 gttcaccaaa aaaattgcaa cgatgacgca tttgcaggaa ggagacaagg ccccggtctt
       61 taccgggaag gatgccgccg gcaacaagat ctcgctggcc gatcataagg gaaagaaagt
      121 ggcgctttac ttctatcccc aggatgatac tcccacctgc acggtgcagg cctgcaacct
      181 ccgcgacaat ttcgccctgc tgaagaagga agggttctcg gtcatcggtg taagccccga
      241 cgatgagaag agccaccaga aattcgggaa gaaattcggg ctgccctttc cgcttgtcgc
      301 cgacgccgat cacaagatca tcgacaagta tggggtgtgg ggggagaagc aattgtatgg
      361 gcggaaatat atggggctgc accgcacgac ctttcttatc gacgagaagg gcgtgatccg
      421 gaagatcttc ctgaagccaa ggagcaagca gcacgcggaa gaaattgtgg ccgcgtgggc
      481 agcggttgcg cccggcaaat gagccgctcc ttcgagtaag ccattccgtc gaatgagcta
      541 ttccgtcgaa taagtcattc cgccgaatga ggcattccgt cgaatgagct attccatcaa
      601 atgagccact ccacttcgcc gaagctgaat tgccggtcga gcacatcccg ggtgagcagt
      661 tccccttgtg cggacacgcc ctgcaccacc gttttgaaga cggcattgtc ttttttcaac
      721 cggacttccc tggcgcggcc atacaagcgc cggttatact gttgaagctg gtcggcggcg
      781 ccgccggcga caagagcgga ataccgctta tccaaaaaaa cgcccaactc ccgggcgagt
      841 tgcaacgaat cgaaggtctt gcccgttatt tgtcgaaggg agacgggatt gcgggccgtt
      901 gacgggaatt ccacctggtt gatattgatc cccatccctg cgatggcaaa cagccagcgc
      961 tctcctttga aatggttctc gataaggatg ccccctgcct ttctgtcatt ccagtaaaga
     1021 tcgttgggcc atttgatcga ggtgtgatcc gcgccggcgt ggtgactgaa gaggtcgtgg
     1081 caggccagcg ccacggcgac gctgagggcg aagacagccg tgggaggcag cgcaactggc
     1141 tctaagacag cactaaggat gatgttctcg cccggtgtgc tggtccagac gcggccccgc
     1201 tggcccttgc cggcccactg gtcatgggcg aaaacaagtg taccgtgatt ggcggccccc
     1261 gcggtggccc gggccatggc atagttgttg gtgctatcca cggattctag ttcaataaaa
     1321 gcgtgaccga tcggcaaaag attcctattt ttggtatgat gtaaaattat gcccggtttc
     1381 tcataaaaat ttgattatca caaaaaacgt tttgattatt ggaacagctt tctatccttt
     1441 ctacccgtaa gcgcagcagc gtggttcgat taacgaggaa ttcgaaaatt tttaagagca
     1501 tcataaaagc gatccaggag aaaaagggcg aaaacatcat ttccctcgac ttacgaaaga
     1561 taccggaggc agtagcggac ttttttatca tttgtgaggc cgccagcaca acccaggtaa
     1621 aggcgatagc cgattcagtg gaagtagaag taaagaacaa ttgcgatgaa ctcccctata
     1681 aacacgaagg cagacaaacc tatcagtggg ttttgatcga ttatgtgaat gtcgtggtcc
     1741 acgtgatgct gccggaaaat cgccgttttt ataaattgga ggacatgtgg agtgatgctc
     1801 cccaggaaga gcatcaggga tagaaaacag ccaggaccga caatttaaca ggttattttg
     1861 ccgtttcatg gatttttttg aaccttttta cccgtataat ttttattatt aataccgcta
     1921 aacaacacgg atacttacaa aaatgccaca agacgatctg aaacaaaacg ataaaaacaa
     1981 ctttccgcgg ttcagaccca ggggagatga cgataacggg cagagaaagg ggcctaaatt
     2041 cagcatatat tggatatggg ggatcattgc agccgtactc atcggattta acttattcgg
     2101 gtctttctca cccgatgcgc acccggcgga tgaactggat tttcgcaaga ccatgctggc
     2161 ccagggtgat gtagaaaaga tcgatctcat ctccaacaag aacctggtcc gggtgtatat
     2221 aaagaaggac agtctcaaca agccctttta ttccaaatac agcaagcaga actcctggcc
     2281 cggcggaatg agcaaggatg gcccccagtt cgaattccgg gtcaccgaca taaaggacta
     2341 tgagaagata ttgcgggaat atttcgccac ccatcccaac caggaggttg cgatcaattc
     2401 caagcaggaa ggcgaatggt ttggcccgct gctgaatttc ctcctcccgc tgctgatcat
     2461 cgtcctcatc tgggtgatgc tgatgcggaa aatgggcggg caggccggca ccggcggtcc
     2521 gggagggatc ttcaatatcg gcaagtccaa ggccaccctc tttgacaagg gaacaaaggt
     2581 gacgatcacc ttcagcgatg tagcgggcct cgatgaggcc aaggtggaag taatggagat
     2641 cgtagacttt ttgaaaaatc ccaagaaata taccgccctc ggcggaaaga tccccaaggg
     2701 tgcgctgctg gtcggtcctc cggggaccgg taagacgctc ctggccaaag ccatggcggg
     2761 cgaagcgcag gttcctttct tttccatgag tggctccgac ttcgttgaac tctttgtcgg
     2821 ggtaggcgcc agccgcgtac gcgatctgtt caagcaggcc cgggagaagg ctccctgtgt
     2881 catcttcatc gacgagatcg acgccatcgg ccgcgcccgc ggtaagaatg ccatcatgag
     2941 caatgacgaa cgcgaaagca ccctgaacca gctgctcgtg gagatggatg gttttggcgg
     3001 tgacagcggc atcatcgtgc tggcagccac caaccgtccc gacgtactcg acaccgcgct
     3061 gctgcgtccc ggccgtttcg accggcagat ctccatcgac aaacccgatc tcaagggtcg
     3121 cgaggccatc ttcaaagtac atctcaagcc gatcaagatc tccagcaaac tggacatcca
     3181 taaattagcc gaacagacgc ccggttttgc cggcgccgat atcgccaatg tctgtaatga
     3241 agccgcgctg atcgcggcgc gtaagggaaa ggaaaccgta gagatggaag atttccagga
     3301 tgcggtggac agggtcatcg gcgggttgga gaagaagaac aagatcatct ctcccgacga
     3361 gaaacgcatc atcgcctatc atgaggccgg ccacgccatc tgcggctggt atctcgaaca
     3421 tgcctatccc cttctgaagg tgaccatcgt cccccgcgga gtcgcggcac ttggctatgc
     3481 ccagtataca ccaaaggaac agtatctcta taataccgat cagctgttcg accagatctg
     3541 tatgacgctt ggcggccgcg ccagcgaaga gctgaatttt gggaagatct ctacgggcgc
     3601 gcagaacgat ctgcagcaga tcaccaagat cgcctattcc atggtaaccg tctacgggat
     3661 gaacgaaaag gtggggaatg tcagcttcta tgatccccag gcggaaaata cgtttaccaa
     3721 gccctactcg gaagaaacct cccggctcat cgacgaagag gtgcggaagc tcatcgacaa
     3781 ggcttacgag gccaccaagc acctgttgac ggacaagacg cgggaagtaa ggctgctggc
     3841 ggaagccctc ctccagcggg aagtgctctt tcaaagtgac gtggaagccc ttatcggcaa
     3901 gcgtccctat gaagagaaac gggcgctgga tgtgtccgaa catgacaacg gggtaccacc
     3961 cgccgtggtt gttccccccg ctaccgaaga agtgaaataa ctatgaaggt atcctccgca
     4021 aaagagaata ttctgaaaaa gataaggcag gcgttgagtg aatcaacgcc tattcctttt
     4081 ccgcaaagtg aaggcagcgc ttccgtcttc catccttcgc ggcaggacct ggaaatagaa
     4141 ttcgccgaac agttcgccaa actgcagggg aaattcgtct tctgtgtgga tcatggcgaa
     4201 ctggtggccc agctcaacgc gcttctcacc agccagggat taaagaagct gtattgccgg
     4261 gagaagaaat tgcgggagac gctgaagacc catgggtttg acgggttctc cgatgtggaa
     4321 ctgggggatt gcgatgccgc cgtcaccacc tgtgaagggc ttgtcgcccg gacgggcagc
     4381 attatcctca gcagcgccgg cgccagcggc cgcacggtca gcgtatatgc gcctgtccat
     4441 atctgtgtag cttataccga tcaattggtc tatgatatcc gggatgggct ggtggggtta
     4501 aaggagaaat accagcagca gctgccctcg ctgatgacct ttgcgacggg gcccagccgg
     4561 accgccgata tcgaaaagac cctggtggtc ggcgtgcatg gaccgaaaca agtgtacctg
     4621 tttttggtgg acagacagta acccaggccg agcaacttat atttgccgca tgcagatccc
     4681 tcccggaaaa aaaatatatt ttctctccga ttttcatctt ggcgcacccg accatgccag
     4741 gagcctggtc cgggagcggc ggatcgtcga cttcctcgaa tcgatccgga aggacgcggc
     4801 cgtgatcttt atcgtcgggg acctcttcga cttctggt
//
LOCUS       DATGFG010001151         4306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1671821, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001151 DATGFG010000000
VERSION     DATGFG010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671821"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1301
                     /locus_tag="VKQ52_21865"
     CDS             102..1301
                     /locus_tag="VKQ52_21865"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HLZ89922.1"
                     /translation="MLSSIESAIEAIKQGKLVIVVDDEDRENEGDFITAARNVTPGII
                     NFMSKEGRGLICAPLVEDRCDELGLELMVNKNTALHETPFTVSVDLLGHGCTTGISAS
                     DRSKTVQALIDPATKPEDLGRPGHIFPLRAKRGGVLRRAGHTEATIDLARLAGFEPAG
                     VLVEIMNEDGTMARLPQLLEIAKKFDLRVISIKDLIEYRLKIDSLIEEVVRVDMPTKY
                     GDFKLIAFREKNTNNEHLALTKGSWQPGEPVLVRVHSSCFTGDILGSFRCDCGEQLHE
                     AMRLVEKEGQGVILYMNQEGRGIGLLNKLRAYKLQEEGMDTVEANIHLGFPMDKRDYG
                     VGAQILRYLGVTKLRLISNNPRKRAGLLGYGLEIVDTVPIEIHPNPHNEKYLQTKRDK
                     LGHEILK"
     gene            complement(1341..>4306)
                     /locus_tag="VKQ52_21870"
     CDS             complement(1341..>4306)
                     /locus_tag="VKQ52_21870"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HLZ89923.1"
                     /translation="TKLHFVNQEVEFDGKFKFNFTGDDIDNFLGHASIYDASLFKNGN
                     RVSFDSLTLESSIEGANKKIVVVSNEFDGAIAGEFSIKDLPASFQTFLNKYYPSYIKP
                     AKTEPANQNFTFVISTKKVDEYIDLFDKDLKGFNNAVIAGRIDTRDKHLLDLNADIPS
                     FGYKNIAFNNLVLKGTGNLDSLSMETTIGDVLLSDSLHFPGTVIHLRSSNDLSSLQVK
                     TSANQTLNSANISTQVRTMPTGVRLTFDPSTFDINSKTWTIDKDGELAFDNNVLSADG
                     MNIHSGDQVISITTHPSGEGNWNDIHVDLKKVNIGDFSPYVVRSERLEGLLTGSADIT
                     NPFGNHTLTFQGQAEQFRFDNDSIGRLDLSADYQKSTGTVHATVHSDNKDYHFDTKAL
                     INLSDSAAGTPIDITTDVAAIKVNMLEKYLGGIFTNLTGNATGKLQITGAPSHLYYLG
                     TVQLRDASLKVAYTQCTYKIPTATIRFKKDTIDFGTLQLKDRLGHTADLLHGRLMHHA
                     FDDLGFDFELNTNRLLLLDTKATDNGQFYGTVIGKAKMTLTGPEQNMIMNIKGEPTDS
                     SNIYLPPSLSRESGEADFIEWRVFGKEMKKSLADRKGSNILVNLDMYANNYANVYMIL
                     DPLSGDIIKTNGHGNLVMRVGTTEDLTLNGRYEIDRGNYNFTFQSFIHKPFILSEGVG
                     NYIQWTGNPYDATIGIAATFRAENVRFSDLGLNNTLGIQDANVLRYRGDIIVTANLTG
                     KLMSPNIAFQIDLPPNSPLKNDQDAQTLLARIQNDPNELNKQVSCLVVLNSFVPLSNS
                     TNTFDPTTAVGNVVVNSISGAISNAFSKQVSQRLQTMFKDKSIRVNFNTNFYNGNYLI
                     FTDASTLAYNRTNLNFSIVKSFMNEKLTMTVGSALDFGMTAQQTQAASFQFLPDINAE
                     YKITPDGRVALTFFYRDSYNYLSTGNHTQNSSGTSISYRREFDRIDELFKKKKKKPPV
                     APVPPPQPTVKNDSN"
BASE COUNT          866 a         1000 c         1374 g         1066 t
ORIGIN      
        1 aaaatggact tatccgcccg ctagtggttt ttgggtagag gcatcccttt ctaactttcc
       61 gccgttttta ttgttaattt tgtacataaa catttttttt gatgctgtcc agtatagaaa
      121 gtgctatcga agccataaaa caagggaaat tggttattgt cgtggatgat gaagaccgtg
      181 aaaatgaagg agatttcatc actgccgccc ggaatgtgac acccgggatc atcaatttta
      241 tgagcaagga aggccggggg ctgatctgtg ccccgctggt cgaggaccgt tgtgacgagc
      301 tcgggctgga gctgatggtc aataagaata ccgcgcttca cgagaccccc tttacggtga
      361 gtgtggacct gctcgggcac ggctgtacta ccggcatctc cgccagtgac cgttccaaga
      421 cggtgcaggc gctgatcgac ccggctacga agccggagga ccttggccgt ccggggcata
      481 tcttcccgtt gagggcaaag cggggtgggg tgctgcgtcg ggcgggtcat acggaagcca
      541 cgatcgacct ggcccggctg gcgggttttg aaccggccgg ggtgctggta gagatcatga
      601 acgaggatgg gacgatggcg cggctgccac agctgctgga gatcgccaaa aagttcgacc
      661 tccgggtcat ttccataaaa gaccttatcg agtatcggct gaagatcgac tcgcttatcg
      721 aggaggtggt ccgggtagat atgcccacca agtatgggga tttcaagctc atcgctttcc
      781 gcgaaaagaa taccaataac gagcatctgg ccctgacaaa gggcagctgg cagccgggcg
      841 agccggtgct ggtaagggtg cactcttcct gtttcacggg agatatcctg ggttctttcc
      901 gctgcgactg tggcgagcag ctccatgagg ccatgcggct ggtggagaaa gaggggcagg
      961 gggtcattct ttatatgaac caggaaggcc ggggcatcgg tctgctgaac aagctgaggg
     1021 cctacaagct gcaggaggag gggatggata cggtggaagc taatatccat ctgggtttcc
     1081 cgatggacaa gcgcgattat ggcgtcgggg cgcagatcct gcgttacctg ggggtcacca
     1141 agctccggct gatcagcaat aatccccgca agcgggccgg cctgctggga tatgggctcg
     1201 agatcgtcga cacggtaccg atcgagatcc atccgaaccc gcataatgag aagtacctgc
     1261 agaccaagag agataaactg ggacacgaga tattaaagta attaaaaacc ccgcttcggc
     1321 ggggttttta attagaatca ttaattggag tcatttttga cggtcggttg cgggggtggg
     1381 accggggcta ccggcggctt tttcttcttc tttttgaaca gctcatcgat gcgatcgaat
     1441 tcccggcggt aggagatgct ggtcccgctg ctgttctggg tatggttacc cgtcgacagg
     1501 tagttatagg agtcccggta aaagaaggtg agggccacgc ggccgtcggg ggtgatcttg
     1561 tattccgcgt tgatatcggg gaggaactgg aaggacgcgg cctgggtctg ttgggccgtc
     1621 atgccgaagt ccagggcgct gcccaccgtc atcgtcagct tctcgttcat aaagctcttg
     1681 acgatggaga agttcaggtt ggtgcggttg taggcaagag tgctcgcgtc cgtgaagatc
     1741 aggtaattcc cattataaaa attcgtgttg aagttgaccc ggatgctttt atccttgaac
     1801 atggtttgca gccgctggga gacctgtttg gaaaaagcgt tggagatggc gccggagatg
     1861 ctgttgacga cgacattacc tacggcggtg gtcgggtcga aggtattggt gctgttggac
     1921 aggggtacga agctattgag cacgaccagg caggagacct gtttgttgag ctcgttgggg
     1981 tcgttctgga tgcgcgcaag cagggtctgg gcatcctggt cgttcttgag cgggctgttg
     2041 gggggaagat cgatctggaa ggcgatgttc ggggacatca gtttgcccgt caggttggcc
     2101 gtgacgatga tatctccccg gtaccgcagg acgttggcgt cctggatacc cagggtattg
     2161 ttgagaccga ggtcgctgaa ccggacgttc tccgccctga aggttgcggc gatcccgatg
     2221 gtagcatcat aggggttacc ggtccactgg atatagttgc ccaccccttc gctcaggata
     2281 aagggtttgt gaataaagga ctggaaggtg aaattatagt tcccgcgatc gatctcatac
     2341 ctgccgttga gggtgaggtc ttccgtcgtc ccgacgcgca tgaccaggtt tccgtggccg
     2401 ttggtcttga tgatatcccc gctcaggggg tcgaggatca tatagacatt cgcatagttg
     2461 ttggcataca tgtccaggtt gaccaggatg ttggagcctt tccggtccgc caggctcttc
     2521 ttcatttctt tcccaaagac ccgccattcg atgaagtcgg cctccccgct ttccctgctg
     2581 agcgatgggg gaaggtagat gttcgagctg tcggtgggtt cccccttgat gttcatgatc
     2641 atattctgtt cggggccggt gagcgtcatt ttcgccttcc cgatgaccgt accatagaac
     2701 tggccgttgt cggtagcctt tgtatccagc aggagcaggc ggttggtatt cagttcgaag
     2761 tcgaagccca ggtcgtcgaa ggcgtgatgc atgagccggc cgtggagcag gtcggcggta
     2821 tggccgagcc tgtcctttaa ctgcagggtg ccgaagtcga tcgtatcctt cttaaagcgg
     2881 atggtcgccg tgggtatctt ataggtgcat tgggtatagg ccactttcag ggaggcgtcc
     2941 ctgagctgta ctgtcccgag atagtagaga tgggaaggtg cgccggtgat ctgtagttta
     3001 ccggtggcgt ttcccgtcag gttcgtgaag atgccgccca ggtatttttc cagcatattg
     3061 acctttatcg cagcgacatc cgttgtgata tcgatgggcg tgccggcggc gctgtccgag
     3121 agattgatca gcgcctttgt gtcgaagtgg taatccttgt tgtccgaatg tacggtcgcg
     3181 tgaaccgtcc cggtggactt ctggtagtca gccgagaggt cgagccggcc gatggaatcg
     3241 ttgtcgaagc ggaactgttc cgcctgtccc tggaaagtaa gggtgtggtt gccgaaggga
     3301 ttggtgatat cggcgctgcc cgtcagcaaa ccttcgagtc tttccgatct gacgacatag
     3361 gggctgaaat cgccgatatt cacctttttc agatcgacat ggatatcgtt ccagttgccc
     3421 tcgccggagg ggtgggtggt gatggagatg acctgatccc cgctgtggat attcatcccg
     3481 tcggcagaga gtacattatt gtcgaaggcc agttcgccgt ccttgtcgat ggtccaggtc
     3541 ttgctgttga tatcgaaagt ggaagggtcg aaggtcagtc tcacgcccgt aggcatggtg
     3601 cgcacctgtg tcgagatgtt ggcggagttg agcgtctggt tggcgctggt ctttacctgc
     3661 aggctggaga ggtcgttgct cgacctgaga tggatcacgg tgccgggaaa gtgcaggcta
     3721 tcgctgagaa ggacgtctcc gatggtggtc tccatggaga ggctgtcgag attgcccgtc
     3781 cctttcagga ccaggttatt aaaggcgatg ttcttatagc cgaaggaggg gatgtccgca
     3841 ttcaggtcga gcaggtgttt gtcccgggta tcgatacggc cggcgatgac ggcgttgtta
     3901 aagcccttca ggtctttgtc gaacaggtcg atatattcat ccaccttctt tgtggaaatg
     3961 acaaaggtga agttctggtt ggcgggttcc gtctttgcgg gttttatata actcgggtaa
     4021 tatttgttga gaaaggtctg gaaggaagcc gggaggtcct tgatggaaaa ttcgccggcg
     4081 atggccccgt cgaattcgtt gctgaccacg acgatctttt tgttggcgcc ttcgatgctg
     4141 gattcgaggg taagcgagtc gaaggacacg cggttgccgt tcttgaacag agaggcgtcg
     4201 tagatgctgg cgtggccaag gaagttgtcg atatcatccc cggtgaagtt aaatttgaat
     4261 ttcccgtcga attccacttc ctggttgacg aaatgcagtt tggtaa
//
LOCUS       DATGFG010001152         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_204185, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001152 DATGFG010000000
VERSION     DATGFG010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204185"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VKQ52_21875"
     CDS             complement(<1..366)
                     /locus_tag="VKQ52_21875"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89924.1"
                     /translation="MAEKKTTSYPKEKINILFLENISDAAVQHFQESGYASVRKLSGA
                     LPEDQLIKEIKDVHLLGIRSKTQITPKVLEAAKKLQAIGCFCIGVNQVDLKAATHSGV
                     VVFNAPYSNTRSVAELVIGL"
     gene            586..1845
                     /locus_tag="VKQ52_21880"
     CDS             586..1845
                     /locus_tag="VKQ52_21880"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLZ89925.1"
                     /translation="MAPAPVTTPALTGPETERYTALTAHFFDPMLAGHFNGSILVAKN
                     GVILFERYKGFRNPVHHQDSINEHTAFHLASVSKTFTAMSTLKLWQEGKLDIHDPVSK
                     YLAGFPYPAITIETLLNHRSGLHNYVHFMDKPGVDKHRLLHNQDVLQYIIDHPNTREI
                     FSGQANRHFEYSNTNYALLALIIEKVSGLPYPEYLAKTFFQPLQMQDTYVFTAPDSGR
                     SMDSYYQTGRPFRMEFLDQVYGDKNVYSTVRDMYKWDQALRSGQLFSKGVLDSAYAGY
                     SFEKPGQRNYGLGWRMLMIPNGKKLIYHNGWWHGNRTVFVRMLDEDATIIALSNNDYK
                     NVYSAKRLCDLFGDYRQGNERFDEGETDPADSTGGATAGEPVPVEKKLVVHTVTHPRL
                     HSHVVTRRATHIAHHKRTTKKKTVQAA"
     gene            1874..3385
                     /locus_tag="VKQ52_21885"
     CDS             1874..3385
                     /locus_tag="VKQ52_21885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HLZ89926.1"
                     /translation="MEILSAEQIRAWDEYTMQHEPIASIDLMERAAASCLAWLEKSGY
                     LDRSFSIYCGKGNNGGDGLALARMLSVLDCPVTVHILEFGNIGTEDFQGNLARIHQTP
                     VEVRFVQGPEHFHPVPPDDILIDALLGSGLNRKLEGVTAQLVDHLNASGNEIIAIDIP
                     TGLFVSRSARGNTVIRATHTLSFQAYKPAFLMPENEAWVGEVHILDIGLHPGFLRQTG
                     SEAELLDPVIIGDIFKPRKSFAHKGNFGHALLVAGSYGKMGAAVLAARACLRTGSGLL
                     TCDIPGCGYGILQTALPEAMLVMDPDDRIHTVVPDGLDRYSVVGVGPGLGQDERTVAF
                     LYTLLQRYRKPVVADADALNILASHPDLLPMLPPYSILTPHPKEFERLFGSSADDYAR
                     LEKAREEARKWQCIIVLKGHYTYIAMPGGKGYFNSTGNPGMAKGGSGDVLTGILTALL
                     GQSYSPGAAAVLGVYLHGLAGDLAADTHSQESMLPSDLTDHLGKAFQIIQKRE"
     gene            3462..3535
                     /locus_tag="VKQ52_21890"
     tRNA            3462..3535
                     /locus_tag="VKQ52_21890"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3496..3498,aa:Pro,seq:cgg)
BASE COUNT          825 a         1081 c          974 g          862 t
ORIGIN      
        1 caggccgatc accagttctg ccaccgaccg ggtattggaa taaggcgcat tgaagaccac
       61 caccccggaa tgggtggcgg ctttcaggtc tacctggttg actcctatgc aaaaacaacc
      121 gatagcctgt aatttttttg cagcttccag gactttgggc gtgatctggg tcttcgagcg
      181 gatgcccagc agatgcacat cctttatttc tttgatgagc tggtcttccg gtaaggcccc
      241 tgacagcttt cggacggatg catatcctga ctcctggaag tgttgaacgg cggcgtcgct
      301 gatattttct aaaaagagaa tgttaatctt ttctttggga taactcgtag ttttcttttc
      361 ggccataatt ttgcccgtct ttttgcgtta gtttccgaaa tggaaatgca aattaacacc
      421 ggattatttt aattttgccg tttccaaccc aaaaattcaa gacaatctta ttagtggaga
      481 agtatgaaag gtaaaggtat tggttattta gtgttgtccg taaccttggt tatcgtatcc
      541 tgtggccagg cgcaaacagc gaaaaatgtt ccagggacgg atacaatggc tcctgctccg
      601 gtcaccactc cggccctgac gggtcccgag acggaacgct acacggccct gacggcccat
      661 ttcttcgatc ccatgctggc cgggcatttc aacggaagta tcctggtagc caagaatggc
      721 gtcatccttt tcgaaagata caagggcttc cgcaatcctg tccatcatca ggactcgatc
      781 aacgaacata ccgcctttca cctggcttcc gtatcgaaga ccttcaccgc catgagcacc
      841 ctgaagctgt ggcaggaagg gaaactggat atccacgatc cggtgtcgaa atacctggcc
      901 ggcttcccct atcctgccat cacgatcgaa accctgctca accatcgcag cggtctgcac
      961 aactatgttc attttatgga caagccgggt gtagataagc atcggcttct tcacaaccag
     1021 gatgtattgc agtatatcat cgaccatccc aatacccggg agatctttag cggccaggcc
     1081 aaccgccatt tcgagtattc caataccaat tatgccctgc tggcactcat catcgagaag
     1141 gtttcgggtc ttccctatcc ggaatacctc gccaaaacct tttttcagcc gcttcagatg
     1201 caggatacct atgtctttac ggcccccgat tccggccgca gcatggactc ctactaccag
     1261 acggggcgtc ctttccggat ggagttcctg gaccaggtgt atggcgacaa gaatgtctat
     1321 agcacggtca gggatatgta caaatgggac caggccttac gctcggggca gttgttctcg
     1381 aagggcgtcc tggacagcgc ctatgccgga tacagctttg aaaagcccgg tcagcgcaac
     1441 tatggcctcg gctggcgcat gctgatgatc cccaatggga aaaaactgat ctatcacaac
     1501 ggttggtggc acggcaaccg cacggtcttt gtccggatgc tggatgaaga cgccacgatc
     1561 atcgccctca gcaataacga ttacaagaac gtgtactccg ccaaaaggct ttgcgatctc
     1621 tttggtgact accgccaggg taacgagcgt ttcgacgaag gcgagaccga tcctgccgat
     1681 agcaccggcg gcgccactgc cggcgaacct gtaccagtag agaaaaagct ggtggtccat
     1741 accgtgaccc atccccgcct ccactcccat gtggttacgc gaagggcaac acatatcgcg
     1801 caccacaagc ggacaacaaa gaaaaagacc gtacaggcgg cgtagttcct cctataattg
     1861 agtaaatttc cgcatggaaa tcctatcagc cgaacagatc cgggcctggg atgaatatac
     1921 catgcaacac gaaccgatcg cctccatcga cctgatggag cgggctgcgg caagttgcct
     1981 ggcctggctg gaaaaatcgg gatatctcga ccggtcgttc tccatctatt gtggtaaagg
     2041 caacaacggc ggcgacggcc tggccctcgc ccggatgctg tctgtcctgg attgcccggt
     2101 tacggtccat atcctcgagt tcggcaacat cggaacggag gatttccagg gtaacctcgc
     2161 ccggatccac cagacacccg tggaagtccg gttcgtccag gggcccgagc attttcatcc
     2221 cgtgccgccg gacgatatcc ttatcgacgc cctgctgggt tccgggctca accggaaact
     2281 ggaaggggtc accgctcaat tggtcgacca tctcaacgct tcgggcaacg agatcatcgc
     2341 catcgacata cccactggtc ttttcgtcag caggagcgcc aggggcaata ccgtcatccg
     2401 tgccacccat acactcagtt tccaggcata taagccggcc ttcctgatgc ccgagaacga
     2461 ggcctgggtg ggtgaggtgc atatcctcga tatcgggctg cacccgggat tccttcgtca
     2521 gaccggcagc gaggcggaac tgctggaccc ggtgatcatc ggggacattt tcaagccgcg
     2581 aaagagtttt gcccacaagg gtaatttcgg tcatgcctta ctggtggcgg gcagctatgg
     2641 taagatgggt gcggccgtgc tcgcggcgcg ggcctgtctg cggaccggca gcggcctgct
     2701 gacctgtgat atacccggtt gcggatatgg tatcctgcaa acggccctcc ccgaagccat
     2761 gctggtgatg gaccccgacg acaggatcca taccgttgtc ccggacggac tggacaggta
     2821 ttccgtcgtc ggcgtagggc ccggtctggg ccaggacgag cggaccgtcg cctttcttta
     2881 caccttgctg cagcgctacc ggaaacccgt tgttgccgat gccgatgcgc tgaatatcct
     2941 cgcctcccac cccgatcttc tcccgatgct ccccccctac tctattctta ccccccatcc
     3001 aaaggagttc gagcggcttt tcgggtcatc cgccgacgac tatgcccgtc tcgaaaaggc
     3061 ccgcgaggag gcgaggaaat ggcaatgcat catcgtatta aaggggcatt acacctatat
     3121 cgccatgccc ggcggcaagg gctacttcaa ttccaccggc aatcccggta tggccaaggg
     3181 cggcagcggc gatgtgctga ccggcatcct gaccgcgctg ctgggccagt cctactcccc
     3241 cggcgcagcc gccgtcctgg gcgtatacct ccatggcctg gccggtgacc tcgccgccga
     3301 cactcattcc caggagagta tgcttccatc cgaccttacc gaccatctcg gtaaagcctt
     3361 ccagatcatt cagaaaaggg aataatggat tttgccgggt gctcaaagga ccttattttt
     3421 gcgtcccgtt tggcggactt ccgccggccg gacatttccc tcgggaagta gcgcagcccg
     3481 gtagcgcact acgttcggga cgtaggggtc gcaggttcga atcctgtctt cccgactttt
     3541 taagttgatt tacaagtaat tagaagctgt tcggacatat ttcggacagc tttttgtttt
     3601 aatggtcttt tcctttacgc accggcttaa ctgcaaaccg aacttcccgc tgaaggatga
     3661 aatcttctcc atgtttcaaa aaatgaagtt tcatttttag tgaacctatc gaagtcaccc
     3721 gaataagggt catgccttgg at
//
LOCUS       DATGFG010001153         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1618956, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001153 DATGFG010000000
VERSION     DATGFG010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618956"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VKQ52_21895"
     CDS             complement(<1..751)
                     /locus_tag="VKQ52_21895"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HLZ89927.1"
                     /translation="MNYQPSQGLQQFKNLVGIKFQLYNSLFTSLPFHRIEKTGILLSL
                     LLTICEEGYKKKQSPEQILEEFFRKHTALVGEREKADMLFRFVQYVERQIVLFDALED
                     AAFRHVNDMSGAGTMKHLLSEVIQTNKEEELTEKLKDYSVRLVLTAHPTQFYPGAVLG
                     IINDLSRAVIENNAAEINLYLQQLGKTPFLKKQRPTPYDEAISLVWYLENVFYHASGR
                     IISALKDQFPHAVSKENALIKMGFWPGGDRDG"
     gene            1110..>2094
                     /locus_tag="VKQ52_21900"
     CDS             1110..>2094
                     /locus_tag="VKQ52_21900"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HLZ89928.1"
                     /translation="MQVLKFGGSSVANADNINRVVALVRQALTKDRTILVVSALGGVT
                     DMLIHAAQSAAAGDESYKDKLQAIEHRHLETVRSLLPLTGQSSVLSAVKTRCNEIEDI
                     CSGVFLLGELSARTQDKIVSYGELLSSLIISARFDAAGVPNTWKDSRELIRTDSRFGH
                     AAVDFVSTNTRIIQYSIDNPTVNLFILPGFIAADSNNITTTLGRGGSDYTAAIVAAAL
                     DAHSLEIWTDVSGMMTADPRLVSNPRLIPNISYQEAMELSHFGAKVIYPPTIQPVMKK
                     GIPVWIKNTFAPAEPGTVIEFDTVNNGGSIRGISSIPKVALLSLEGSGMVGI"
BASE COUNT          494 a          684 c          450 g          466 t
ORIGIN      
        1 tgccgtcacg gtcgccgccg ggccaaaaac ccatcttgat cagcgcattt tccttgctca
       61 ccgcatgcgg gaactggtct ttcaacgccg aaatgatccg cccgctcgcg tggtaaaaga
      121 cattctccaa ataccagaca aggctgatcg cctcgtcata aggcgtgggc cgctgtttct
      181 tcaaaaaagg cgttttcccc aactgctgca gatacagatt gatctccgcc gcattattct
      241 cgatcaccgc ccgtgaaaga tcgttgatga tacccaacac cgcgccggga tagaactgcg
      301 tcggatgcgc ggtcagcacc agccgcacgg aataatcctt cagcttctcc gtcagctcct
      361 cctccttatt ggtctggatc acctcggaca acaaatgctt catcgtgccc gcaccgctca
      421 tatcgttcac atggcgaaat gccgcatcct ccagcgcatc gaaaagcacg atctgccgct
      481 ccacgtattg tacaaaccgg aaaagcatat ccgccttctc ccgctcaccc accagcgccg
      541 tatgcttccg gaagaactcc tccaggatct gctccgggct ctgcttcttc ttatacccct
      601 cctcgcaaat ggtcagcaaa agcgacaaca ggatacccgt tttctcgatt cggtgaaaag
      661 gcagggaggt aaataaacta ttgtataatt ggaattttat acccaccagg ttcttgaact
      721 gctgcaaccc ctgggacggt tgataattca taacggaagt tttttgtctg tcctaacgga
      781 cgaaccgcct ccggcggttc tggtttaata ctattcaaag aatgcgccta atacacagcg
      841 aatgttcaaa accttcgcga aattacttta tttgcactaa gaaataggca tttttttgtt
      901 tttacattca tttctcccta ccttagcgtc gtgattctcc atcaccacca taccggctct
      961 tcctccaccg ttgttgaaat tacaactatt atctccatta caacccgtta agggttgtca
     1021 atttccatat tacccccggg gctttatagt agccatccct cagcaggacg tcattctcca
     1081 ttacttcaaa aagacaaatc aactagtgta tgcaggttct aaaattcggt ggttcttcgg
     1141 tagccaacgc ggataatatt aacagggtcg tcgccctggt cagacaggca ttgacaaagg
     1201 acaggaccat cctcgtcgtt tcagcgctgg gcggcgtcac ggacatgctg atccatgccg
     1261 cccaatccgc ggccgccggc gacgaatcct acaaagataa attacaggcc atagaacacc
     1321 gccacctcga aaccgtccgc tccctcctgc ccctcaccgg ccaaagcagc gtcctcagcg
     1381 cggtaaagac ccgttgcaac gagatcgaag acatctgcag cggcgtattc ctgctcggcg
     1441 aactctccgc ccgcacccag gacaagatcg tcagctatgg tgaattgttg tcctccctca
     1501 tcatctccgc ccgcttcgat gccgccggcg tccccaatac ctggaaggat tcccgcgaac
     1561 tgatccgcac agactcccgc ttcggccatg ccgccgtgga cttcgtcagc accaacaccc
     1621 ggatcataca atacagcatc gacaacccta ccgtcaacct cttcatcctg cccggcttca
     1681 tcgccgccga cagcaacaat attaccacta ccctcggccg cggcggctcc gattacaccg
     1741 ccgcaatagt ggccgccgcc ctcgacgcgc attcactcga gatctggacc gacgtctccg
     1801 ggatgatgac cgccgacccg cgtctcgtct ccaacccccg cctgatcccg aacatctcct
     1861 accaggaagc catggagctg tcccatttcg gcgcaaaggt catctatccg ccaaccatcc
     1921 agcccgtgat gaaaaaaggc atccccgtct ggataaagaa caccttcgcc cctgccgaac
     1981 cgggaaccgt catcgaattc gacaccgtga acaacggcgg cagcatcagg ggtatctcca
     2041 gtatcccaaa agttgccctg ctcagcctcg aaggcagcgg catggtcggt atcc
//
LOCUS       DATGFG010001154         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_698570, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001154 DATGFG010000000
VERSION     DATGFG010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698570"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VKQ52_21905"
     CDS             complement(<1..769)
                     /locus_tag="VKQ52_21905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLZ89929.1"
                     /translation="MNLPGLYDRALNFEFLDAEEGIFLFEQAPLTELMYVANELRKKQ
                     VPHGRVTWQIDRNVNTTNVCFANCKFCNFYRVPGHADAYITDIDTYKRKIEETFRWGG
                     DQLLLQGGHHPELGLSFYTTLFRQLKELYPTLKLHTLGPPEVAHICKKEKMSHHDVLS
                     ALKAAGMDSLPGAGAEILVDRVRRLISKGKCGAQEWLDIMHEAHKLHITSSATMMFGH
                     VETLRERMDHLISIREVQAKKPATAKGFLAFIPWTFQD"
     gene            892..>2029
                     /locus_tag="VKQ52_21910"
     CDS             892..>2029
                     /locus_tag="VKQ52_21910"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HLZ89930.1"
                     /translation="MLSYEKHLSLPYCIRPRLYRFALLLLTAFGSMTGGTLGAQETFV
                     QPAAKMIATFPFTVFTGGVILLKAKLNDFPDSLNFILDTGSGGISLDSGTCVRLRLTP
                     QPSDKTILGIAGVRQVKFLYNQVLHLPGLDVDSLNFHVNDYDILTSVYGEKVDGIIGY
                     SFFSRYIVQINYDSNRVSVWTRGNFRYPKGGYMMRPVIASLPILGAEVRDAQKIFARF
                     YFDTGAGLCALFSTDFVSDSNLLDSRKKVYYTSAQGLGGKAMMRLTTIRDLRLGPYRF
                     HNVPSYVFDDQYNVTSYPNLGGLVGNDILRRFNVTLNYDRRVIYLVPNSHYRDQFDYS
                     YTGLGLYWMNGEVRVGDVMKDSPAERAGLKEDDIVLSVGNNFSNN"
BASE COUNT          421 a          488 c          592 g          528 t
ORIGIN      
        1 cgtcctggaa ggtccagggg atgaaggcaa ggaagccttt ggcggtggcc ggtttttttg
       61 cctgtacctc cctgatgctg atgaggtgat ccattctttc ccggagggtc tccacatgac
      121 cgaacatcat ggtggcggag ctggtgatat gcagtttgtg ggcttcgtgc atgatgtcga
      181 gccattcctg cgccccgcat ttgcccttac tgatcaggcg gcgtacccgg tcgacgagga
      241 tctccgcgcc tgcgccgggc agggagtcca tacctgcggc ttttagcgcg gagaggacgt
      301 cgtgatggct catcttttct tttttgcaga tatgcgccac ttcgggtggc ccgagggtgt
      361 ggagcttcag ggtgggatac aattctttca gctgccggaa cagggttgta tagaagctga
      421 gtcccagttc ggggtggtgt ccgccctgca ggagcaactg gtcgccgccc cagcggaagg
      481 tctcttcgat ctttcgttta tacgtgtcga tatcggtgat ataggcatcg gcgtgaccgg
      541 ggacgcgata gaaattgcag aatttgcaat tggcgaaaca gacgttggtg gtattgacgt
      601 tgcggtcgat ctgccacgtg accctgccat gtggaacctg tttttttcgc agttcgtttg
      661 cgacgtacat caattccgtg agcggcgcct gttcgaaaag gaagatgcct tcttcggcgt
      721 cgaggaattc gaagttcaat gccctatcat ataagcctgg cagattcata actgtgcttt
      781 gtggggacaa aggtacgcaa ccgcggtcgc ttgaaaaaag cggatattct tcgatgaacg
      841 gtttgaaaac ctaacaaata gagattatat ttaatggacc aatgtctaac tatgcttagc
      901 tacgagaaac atctttcact gccctattgt atccgccccc ggttgtaccg tttcgcgttg
      961 ttgttactga ccgcatttgg ttccatgacc ggaggcacgc ttggcgccca ggaaaccttt
     1021 gtccagcccg ctgccaaaat gatcgccact ttccctttta cggtattcac cggtggcgtc
     1081 atcctgctga aggcaaagct gaatgatttc cccgacagtc tcaatttcat ccttgatacc
     1141 ggcagtggcg ggatctcgct ggattccggg acctgtgtgc ggctccggtt gacgccgcag
     1201 ccatcggata agaccatcct cgggatcgcg ggggtgcggc aggtgaagtt cctgtacaac
     1261 caggtccttc atctcccggg actcgatgtg gacagcctga attttcatgt caacgactat
     1321 gatatcctga ccagcgtata tggagaaaaa gtagacggga tcatcgggta cagcttcttt
     1381 tcgagatata tcgtccagat caattatgac agtaaccggg tttcggtctg gacccggggc
     1441 aatttccggt atccaaaagg cgggtatatg atgcggccgg tgatcgccag tctgcccatc
     1501 ctgggtgcgg aggtgaggga tgcgcagaag atatttgccc gcttctattt tgatacgggg
     1561 gcagggcttt gtgctttgtt ctcgactgat ttcgtcagcg acagcaatct tcttgacagc
     1621 cggaaaaaag tgtactatac gtcggcgcag gggcttggtg gaaaggccat gatgcggctg
     1681 acgacgataa gagatcttcg gctcgggccc taccggtttc acaatgttcc gagctatgtc
     1741 tttgacgatc agtataatgt cacgtcctat cccaatcttg gcgggttggt gggaaacgat
     1801 atcctccggc ggttcaatgt gacgctaaac tatgatcgcc gggtcattta tcttgtcccg
     1861 aattcgcatt atcgcgacca gttcgactat tcctataccg ggctggggct gtattggatg
     1921 aatggcgagg tccgggtcgg ggatgtaatg aaggattcgc ctgccgagag ggcggggctg
     1981 aaggaggatg atatcgtgtt gtcggtgggg aataatttca gcaataata
//
LOCUS       DATGFG010001155         5072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_619952, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001155 DATGFG010000000
VERSION     DATGFG010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619952"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4174)
                     /locus_tag="VKQ52_21915"
     CDS             complement(<1..4174)
                     /locus_tag="VKQ52_21915"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HLZ89931.1"
                     /translation="MAALVLLYQAAFSQVESPSTLDRPALAAFLQRVVKDKAGAFEVA
                     YLAPEGGKDVFEVESREGGMTGRGEVGKAGRGEVGAVGGGEGGAARKIILRGSNGVSI
                     ASALNYYLRHYCHYLITWNGSSKQLPAVLPVVKERVHRRTPYDYRYYLNYCTFNYSMA
                     WWNWDRWQREIDWMAMNGINMPLALTGEEAIWQEVYRGMGFTDAELDRFFTGPAYFSW
                     LWMGNIDAWGGPLPAHWKESHKALQKKILSAERAFGMKPVLPAFTGHVPPAFRERFPA
                     AKVKKTNWDAGFGDVYILDPGDPMFVEIGRRFLEVQTREFGTDHLYSADTFNENVPPT
                     TDSTYLDAMSKKVFASMAAADPSAVWVMQGWMFHYNAAYWQPVQIQALLKAVPDDHMI
                     LLDLYSESHPVWNRTAALGAAGSGSSEGQTSAVRRDRTTFPIKADAGAYYGKPWIWNM
                     LHDFGGNNPMWGRMSHAAADPSAALHDGASGKMVGIGLTPEGIEQNPALYQLMLENVW
                     REGPVDVGQWLKEYARERYSGGGVRGAGVSGQVGADRGGGVDERVWADVDEAWRLLEG
                     SVYRGGLGEGGPESIIQARPTFEKKIDRVLTGLDYDPAVLVKALGSFVRAAPVLGKSE
                     GFRYDLVDMTRQVLANYASPLQQAFVEAWKRGDSVAYHRDSRKFLELMDDMDRLLAAQ
                     KGFLLGKWIRDARACGVSPAEKDLYEKNARDLITLWGDKESGLREYSNRQWSGLISGF
                     YKRRWELLFARLDKGIGSGVMDWAAFDKEVKDWEWKWVNGREVYSDEPKGDAVAVAVG
                     LYKKYAALAAGNAGEGAGSAVEPVGSAVVGTVGAGEAAVSNDHIFPAAAVARPYIDFD
                     GKGFLVKGKRCFLVSAGMEYARVPHELWRDRLQRLQRAGFNCIEIYTFWNFHEPQEGK
                     FDFGGDHDLEAFLRLVGEMGMYAIVRVGPYYCAEWDNGGYPLWLRFKPGVRVREDNAE
                     FLGYVDRFFDRLLPIVCRQQIRFGGPVILVQLENEHNLGWGTVMPNGYFRHLREKALS
                     KGLEVPYFFSGLHHASDPAGDVGPAAGAAGDMGQTADAASTGGGDMGRRGDVMEAGGD
                     IGRKGDPARLDDPGRPNPWFSTEFWSVWYNGYGPGAADSAVYARRTWKIIAHGGGGYN
                     YYMAHGGSNFGYTNNDEDAASYDYGAAVGQAGDLRPMYYAFKRAALFARSFQGVLANG
                     VDATAQWGDILRDRSVRVTARHSGMGDVVFLDNPGERPVRTEVRGVPVVLAPGEILPV
                     VHRFVVSPLITLEWAPVRVVGITGAGAFRTMVVEGEEGSVARLELLIGGRRRKLDTIV
                     SAAPEEFFFRAGGETLRVLVVSRRMADQTWFVDSGYVICGADYVGDMRVRGRAVSVVT
                     ETRENGVPAGVLW"
     gene            complement(4309..>5072)
                     /locus_tag="VKQ52_21920"
     CDS             complement(4309..>5072)
                     /locus_tag="VKQ52_21920"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLZ89932.1"
                     /translation="VWNKNQEISNVPASAISTYFTPRAWQGWGFHHPTENFAEEFEPA
                     DSIRKKATLISVGDSIPNQTNMTTIGSGDAYQQFTGLTGASTGRMLPSMSTTGYCIKK
                     YTAFMPSGDGGVDYDLKQPLLRSSDVYLLVAEARIRLNGAGSGDAEINAVRKRAGLAP
                     VAGAGMNQLIHERRVELAGENIRWQDLLRWDKDKLINLDTIVGKAKTASPLAPYNGTT
                     IIPSRTFTRPKDYYMPIPQEIIDESKGVIKQNSNY"
BASE COUNT         1156 a         1944 c         1016 g          956 t
ORIGIN      
        1 accaaagcac acccgccggt accccattct cccgcgtctc ggtcactacc gaaaccgcac
       61 gtcctcgcac ccgcatatcc ccgacataat ccgccccaca aataacatat ccactatcga
      121 caaaccaggt ttgatccgcc atccgcctgc tcactaccaa cacccgcaag gtctctcctc
      181 cggcccgaaa gaaaaattcc tccggcgccg ccgacacaat cgtatccaat ttcctccgcc
      241 tacccccgat aagcaactcc agtcgcgcca cactcccttc ctccccttcg accaccatcg
      301 tccggaatgc cccggccccc gtgatcccca cgactctaac cggcgcccac tcaagggtga
      361 taagcggact cacgacaaaa cgatgcacca ccggcaatat ctctcccggc gccaaaacca
      421 ccggaacacc tctcacctcc gtccgcaccg gccgctcgcc ggggttatcc aaaaacacca
      481 catcccccat cccactatgc cgcgccgtca cccgcaccga cctgtcccga aggatgtccc
      541 cccattgcgc cgtcgcatcc accccattcg ccagcacccc ctgaaaactc cgggcaaaca
      601 acgccgcccg cttgaatgca taatacatcg gccggagatc accagcctgc ccaacagccg
      661 ccccataatc ataagaggcc gcatcctcgt cattattcgt atatccaaaa ttgcttcccc
      721 catgcgccat ataatagttg tacccgccac cgccatgcgc aatgatcttc caggtccggc
      781 gcgcatacac ggccgaatcg gctgctcccg gcccataccc gttataccat acactccaaa
      841 actccgtcga gaaccaggga ttgggcctgc ccggatcatc caacctcgcc ggatcgccct
      901 tccgcccgat atcaccaccc gcctccatca catcccccct tcgccccata tctcccccgc
      961 ccgtactcgc tgcgtcagcc gtctgcccca tatcaccagc cgcacccgcc gccggcccca
     1021 catcacccgc cggatcactg gcatggtgca gcccactaaa aaaataaggg acctcaagtc
     1081 ccttcgacaa cgctttctcc cgcaaatgcc ggaaatagcc gttcggcatc accgtccccc
     1141 accccaggtt gtgctcattc tccaactgca caaggatcac cggaccgcca aaccggatct
     1201 gctgccggca aacgattggt aacaaccggt caaagaaccg gtccacatag ccaaggaatt
     1261 ccgcattgtc ctcccgcacc cgcacccccg gcttgaaccg taaccataaa ggataaccgc
     1321 cgttatccca ttcggcgcaa tagtaaggcc ccacccgcac gatggcatac atccccatct
     1381 cacccaccag ccgcaaaaaa gcctccaggt catgatcccc gccgaaatcg aactttccct
     1441 cctgcggctc atgaaaattc cagaacgtat agatctcgat acaattaaaa ccagcccgct
     1501 gaagacgctg caaccggtcc cgccaaagtt catgcggcac ccgcgcatac tccatccccg
     1561 ccgacacaag aaaacaacgc ttgcccttca ccaaaaaacc cttcccgtca aaatcgatat
     1621 acggcctggc caccgccgcg gccggaaaaa tatgatcatt cgacaccgca gcctcacccg
     1681 cacccaccgt tcccacaaca gcgctcccca ccggctcaac agcgctcccc gctccctcac
     1741 cggcattccc cgctgccaat gccgcatact tcttatataa cccgaccgcc accgccacgg
     1801 catccccctt cggctcatcg ctgtacacct cacgcccatt cacccatttc cactcccaat
     1861 cctttacctc cttgtcaaaa gccgcccaat ccatcacccc gctgccgatc cccttgtcaa
     1921 gccgcgcaaa caataactcc catcgccgtt tataaaaacc gctgatcaaa cccgaccact
     1981 gccggttgga atattcccgc aacccgctct ccttatcgcc ccacaacgtg atgagatccc
     2041 gcgcattctt ttcatacagg tccttctcgg ccggactgac cccacaagcc cgggcatcgc
     2101 gtatccactt cccaagcaaa aaccccttct gcgcggccaa cagccggtcc atatcatcca
     2161 tcaactccaa aaacttccgg ctgtcccggt gataggccac actgtccccc cgcttccacg
     2221 cctccacaaa cgcctgctgc aatggactgg cgtaattggc cagcacctgc cgcgtcatat
     2281 ccacgagatc ataccggaac ccttcgctct tccccaacac cggcgcagcc cgtacaaacg
     2341 atccaagcgc cttcaccagc accgccggat cataatccag ccccgtcagc acccggtcga
     2401 tcttcttctc gaacgtcggc ctcgcctgga tgatcgattc cggcccgcct tctccgagtc
     2461 cgccccggta aacactccct tccagcaacc gccacgcttc atccacatcg gcccataccc
     2521 gctcatccac tcccccaccc cgatctgccc ccacctggcc actcacgccg gcaccgcgaa
     2581 cccctccccc actatatcgc tcccgcgcat actccttcaa ccattgcccc acatctaccg
     2641 gtccctcccg ccatacattc tccagcatca gctgataaag cgcgggattc tgctcgatcc
     2701 cttccggcgt cagcccaata cccaccatct tccccgaagc cccatcatgc aaggccgcgg
     2761 acgggtccgc agcagcatga ctcatccgcc cccacatggg attattgccg ccgaaatcgt
     2821 gcagcatatt ccagatccag ggcttcccat aataagcgcc ggcatcggcc ttaataggaa
     2881 aagtagtccg atcccggcgc accgctgatg tctgtccttc ggacgagccg cttccagcgg
     2941 ctcctaaagc cgccgtccgg ttccacacag gatggctctc gctgtagaga tccaggagta
     3001 tcatatgatc atccggcacc gccttcagca aggcctggat ctgcaccggc tgccagtacg
     3061 ccgcattata gtggaacatc caaccctgca taacccacac cgccgacgga tcggccgccg
     3121 ccatcgaggc aaacaccttc ttgctcatcg catcgagata ggtcgaatcc gtagtcggcg
     3181 gcacattctc attgaaagta tcggcggaat acaaatgatc cgtcccgaac tcccgcgtct
     3241 gaacctcgag gaaccggcga ccgatctcca cgaacatcgg atcacccggg tccaggatat
     3301 acacgtcccc gaatcccgca tcccaattcg tttttttcac cttcgcagcc gggaacctct
     3361 cgcgaaaagc cggcggcaca tgacccgtga acgcaggcaa caccggcttc atcccaaaag
     3421 cccgctccgc cgacaggatc ttcttctgca aagccttgtg actctccttc cagtgtgccg
     3481 gcaaaggacc gccccaggca tcgatattcc ccatccataa ccaggagaaa taagccggac
     3541 cggtaaagaa ccgatccagt tcggcgtcgg taaaacccat tccccggtac acctcctgcc
     3601 agatggcctc ctcacccgtc aacgccagcg gcatattgat cccattcatg gccatccagt
     3661 cgatctcccg ctgccagcga tcccaattcc accaggccat ggaataattg aacgtacaat
     3721 agttgagata atagcggtaa tcgtacggcg tcctccggtg aacccgctcc ttcaccaccg
     3781 gcaacaccgc cggcaactgc ttcgaactcc cattccacgt aataagataa tgacaataat
     3841 gccgtaggta ataattcaac gccgaggcaa tacttacccc attactccca cgaagaatga
     3901 tctttcgcgc cgccccgccc tcaccgcccc caaccgcccc aacttcaccg cgtccggcct
     3961 taccaacttc accgcgtccg gtcatcccgc cttcacggct ctccacctcg aacacatcct
     4021 ttcccccctc gggcgcaaga taagccacct cgaaggcccc ggccttatcc ttcaccaccc
     4081 gttgcaaaaa agcggcaagc gccggccggt ccaatgtcga cggactctct acttgtgaaa
     4141 aagcggcctg gtacaaaagc accagcgccg ccacaattgt gatgcgaaaa aaaattgatg
     4201 aaaaataatt ttttttcgca tcatgcacaa tgtctgtaca cttcggcccg gggcctcgtg
     4261 tactaactgg gctccgccca gttatggcca actttcgcat ttaatatatt aataatttga
     4321 gttttgcttg atcaccccct tgctctcgtc gatgatctcc tgcgggatcg gcatataata
     4381 atctttcggc cgtgtaaagg tacgggaagg aatgatagtc gtcccattat acggcgccag
     4441 cggagaagcc gtcttggcct tgccgacgat cgtgtccagg ttgatcagct tgtccttgtc
     4501 ccaacgcaac aggtcctgcc aacggatatt ctcccccgct aactccaccc ggcgctcatg
     4561 gatgagctga ttcatccccg cacccgccac cggagcgagc cccgcccgct tccggacggc
     4621 gttgatctcc gcatccccgc tccccgcacc attgagccgg atcctggcct ccgccacaag
     4681 caggtagaca tccgaactgc gcagtaaggg ctgcttcaga tcgtagtcga cacccccatc
     4741 cccgctcggc ataaaggccg tgtacttctt gatacaatag cctgtagtgg acatgctcgg
     4801 caacatccgg cccgtcgaag cacccgtcag ccccgtgaat tgctgatagg catcccccga
     4861 accgatcgtc gtcatattgg tctggttagg gatcgaatcc cccaccgaga tcagcgtcgc
     4921 cttcttccgg atcgaatcgg ccggctcgaa ctcctcggca aaattttccg tcggatggtg
     4981 aaaaccccaa ccctgccagg cccgcggcgt aaagtacgta ctgatcgccg atgcaggtac
     5041 attggatatc tcctggttct tattccacac cg
//
LOCUS       DATGFG010001156         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1672017, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001156 DATGFG010000000
VERSION     DATGFG010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672017"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VKQ52_21925"
     CDS             complement(<1..1010)
                     /locus_tag="VKQ52_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89933.1"
                     /translation="MKRFLPYLRESWIFLPAIPFFVIVLQNAPNIPIMDDYNAILNFL
                     TDWKKAGFMERLSLLFLQHNEHRILHSRMIFALYYTIFGNVNFRALIILADLQLLVIA
                     AVAAYFIRKVGGKYWRIMAFLWTLCIFDLNTYEAGSMAMTGMQNYGVIMLFFLSLFFY
                     DRSRRWLPLAAILQVLCIFSSGGGMIGAFFVVVYTLRLGDKWKKMVSISTAAVFIPLY
                     FVHYTFVSQPNKLPFSINTVVTYFIRMTGAPVNFDASLYFGLLILAGLIALFVIKPVK
                     APWAILCIFGYVLGSMATSALFRSCLKGAQFQTSRYLIYPQLLLAMACLLIWLKVETK
                     KF"
     gene            complement(1327..>2467)
                     /locus_tag="VKQ52_21930"
     CDS             complement(1327..>2467)
                     /locus_tag="VKQ52_21930"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HLZ89934.1"
                     /translation="KYNYQGDKYFIPASNTKLFSLYAGLRYLGDSLVGMRYRVTDTAL
                     FILPSGDPTLLHPSFPDQPVIRWLQNAHRNLYLTEGNWEDRPFGRGWAWDDYNDDYMP
                     ERSSFPVYGNLIRWLQDTISGSFFSIPDIDWPVRFSPDSSKQGFSVRRDYHDNSFVIT
                     EGKGRVHEQDVPFITDGMAGAAKLLKDTVGRAVGIRQRIPGTGTAGVVHSRPVDSLFR
                     PMMYNSDNFFAEQTLLMAGNELLGKMNDGRIIDTLLNGELKDLPQRPIWVDGSGLSRN
                     DLFTPQDFVWLLNKMSRDFGLDRMKGILPTGGKGTLSSYYKQDSGFIFAKTGSLSGVV
                     ALSGYLITKKNHLLLFSILINNYTGSGVTVRRQMEQWIHRIRDTY"
BASE COUNT          638 a          655 c          661 g          513 t
ORIGIN      
        1 tggaattttt ttgtctccac cttcagccag atcaacagac aggccatcgc cagcaatagc
       61 tgcggataaa taagataccg ggaggtctgg aactgcgcac ccttcaggca ggagcggaac
      121 aaagcggatg tcgccatcga accgagcacg taaccgaaga tacacagtat cgcccaggga
      181 gccttcaccg gttttatcac gaacagggca ataagccctg ccaggatcag caacccaaaa
      241 tacaggctgg cgtcgaaatt gaccggcgcc ccggtcatcc ggatgaaata ggtcaccacc
      301 gtgttgatac taaacggtaa tttgttgggc tgcgagacga aggtataatg cacgaagtac
      361 aggggaatga agacggctgc cgtcgatata ctcaccatct ttttccattt gtcccccagc
      421 cgcagcgtat aaacgacgac gaagaatgcg ccgatcatcc cgccgccgct ggaaaaaatg
      481 cacaatacct gcaggatggc ggccagcggc agccatctcc tgctccggtc gtaaaagaac
      541 aggcttaaaa agaaaagcat gatcacccca tagttctgca tgcccgtcat cgccatgctt
      601 cccgcttcgt acgtattcag atcgaatatg cacaaggtcc acagaaaggc catgatccgc
      661 cagtattttc cgcccacctt ccggataaaa taggcggcca ccgccgcgat caccagcagc
      721 tgcagatcgg ccaggatgat cagggcccgg aaattgacgt tgccgaagat cgtatagtac
      781 aaggcaaaga tcatccggga atgcaggatc cgatgttcgt tatgctggag gaaaagaagc
      841 gatagccttt ccataaaacc ggccttcttc cagtcggtca gaaagttcag gatcgcgttg
      901 taatcatcca tgatggggat gttcggcgca ttttgaagga caataacaaa aaaggggatc
      961 gcgggcagaa atatccagga ttccctgaga taaggcagaa agcgtttcat ggtcgtaagg
     1021 ttaggcggtg aataaggtat agagcagcat caaaccggat gaatacagct tcaaaatagc
     1081 gaaaggcgta tatacgtgct agtggctaaa aaacccgctt tttaccatcc ggcggggggc
     1141 cggcaaccgg ctatgtagat ggctgtgccg ttttagcgga attgcgtgtc gtctctgacg
     1201 acatccgtgt cgcccgaata gtagaaggcc caaaaaaaag cacgaaaaaa aacccaaaaa
     1261 tcgagggttt ttacgcgtgt tttatgtggt tttccgaaga tgtacggtgc aatcgcagta
     1321 atttttttaa tatgtatccc tgatccggtg gatccattgc tccatctgcc gccggacggt
     1381 gacgccgctg cccgtgtagt tattgatcag gatggagaat aaaagcaggt ggttcttttt
     1441 agtgataagg tatccgctaa gcgcaacgac ccccgacagg ctgccggtct tggcaaaaat
     1501 aaagccgctg tcctgtttat aataggagga tagggtgccc ttcccacccg tcggcaggat
     1561 acccttcatc cggtcgaggc caaaatccct gctcatcttg ttcagcagcc agacaaaatc
     1621 ctgcggggta aaaagatcgt tacggctgag cccgctgcca tcgacccaga tgggccgctg
     1681 cggcaggtcc ttcagttcgc cgttaagcag ggtatcgatg atccggccat cgttcatctt
     1741 ccccaacagc tcattgcccg ccatcagcag cgtctgctcc gcaaaaaaat tatcgctgtt
     1801 atacatcatc ggccggaaca gggaatccac cggtcgcgag tgaacgacgc cggcagtacc
     1861 ggtgccgggg atgcgctgac ggatgcccac cgccctgcca accgtatcct ttaacagctt
     1921 ggccgcgccg gccataccat ccgttataaa gggaacatcc tgctcgtgga cccggccctt
     1981 cccttcggtg atcacaaaac tgttgtcgtg gtagtcgcgc cggacggaaa agccctgttt
     2041 cgaggagtcg ggcgagaacc gtaccggcca gtcgatatcg ggaatggaaa aaaaggagcc
     2101 ggatatggtg tcctggagcc agcggataag attgccatac acggggaagc tactgcgctc
     2161 gggcatatag tcgtcgttat agtcatccca ggcccagccg cggccaaagg gccggtcctc
     2221 ccagttccct tccgtgagat agagattccg gtgcgcgttt tgcagccacc ggatgacggg
     2281 ctgatcggga aatgacggat gcaacagcgt aggatcgccc gaaggcagga tgaacaacgc
     2341 ggtatccgtt accctgtatc gcatacccac gagcgagtcc cccaggtatc gcaacccggc
     2401 atacagggaa aagagtttgg tattgctggc cgggataaaa tatttatccc cctggtaatt
     2461 gtattta
//
LOCUS       DATGFG010001157         3572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_873154, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001157 DATGFG010000000
VERSION     DATGFG010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873154"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VKQ52_21935"
     CDS             <1..235
                     /locus_tag="VKQ52_21935"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ89935.1"
                     /translation="VLQFFRPINTARLQYHLSDREKEVLNYLVKGYSYKMIAGYCHIS
                     LDTVRGHIRNIYSKLHVNCGREAVSKALRDNIL"
     gene            298..990
                     /locus_tag="VKQ52_21940"
     CDS             298..990
                     /locus_tag="VKQ52_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89936.1"
                     /translation="MQHPLSELSASPRRRFLGKIAAGAAALAVLDIPFKLSAAPSTPA
                     VGPSDPDAWFSQLKGKHRLILDVPRPNELFPFAWPRVFLLTNAATGTPEKENNVVVVL
                     RHEGIPFAFEDRIWAKYKFGEVFKVNDPKTKAPATRNPFWKPMPGDFTIPGIGKVDIG
                     IDQLQSSGVLFCVCDVAMTVYSAAIAGDSDPAEVKKDWMSGLLPGIQPVPSGIWAVGR
                     AQEHGCTYCYAG"
     gene            1080..1922
                     /locus_tag="VKQ52_21945"
     CDS             1080..1922
                     /locus_tag="VKQ52_21945"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLZ89937.1"
                     /translation="MWEAPDTARIPATDEGRLIRYGRDLIANTSHYLGPKGIKAAITN
                     GMNCQNCHLNAGTKPWGNNYSAVFSTYPKYRDRSASVEDIHRRVNDCIERSLNGHPLD
                     TNSLEMKAISAYIKWLGQNVPKGVKPPGAGIRDLPWLTRAADPEKGRTVYRQNCRRCH
                     GTEGGGSPNPDNSGYVYPPLWGKHSYNTGAGLYRISRLAGYVKDNMPLGCTHNTPLLT
                     DEQAWDVAAFILCQNRPEKAFPGDWPDLTRKPVDHPFGPYADSFTASQHKYGPFGPIR
                     QVHP"
     gene            2007..2606
                     /locus_tag="VKQ52_21950"
     CDS             2007..2606
                     /locus_tag="VKQ52_21950"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLZ89938.1"
                     /translation="MVWYENEVQRLEQERAALTYEPKLLFYGSSSIRLWESLNEDFAG
                     YQPVNLGFGGSTLAACDWFFDRLVAPFHPEQIIVYAGDNDLGDGRHPEEIFIFFQQLV
                     SRTRKQFGDIPLAFISIKPSITRWNIVDSIRYANKIIEDEIKRQANHLYFIDIYHRMT
                     DNSGYPKREFLDPDGLHINAKGYALWKEIILQYLSARTT"
     gene            2603..3331
                     /locus_tag="VKQ52_21955"
     CDS             2603..3331
                     /locus_tag="VKQ52_21955"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLZ89939.1"
                     /translation="MKREYYKWFSKPLGRDMELLVFGHAGDPVLFFPTRTARFYDYED
                     WQVIEALRKKIETGSIQVYCLDSVDKESFYAKDLHPAQRICRHLDYEKYVLNEVLDLI
                     QQKNPHPTVIAAGCSLGAYHAANLAFKYPWLFHKMVGLSGRYDLTTTMEYFDDLFEGY
                     VDENIYYNMPGRFIPNLQDQQLISLLKAMEIIFVVGEKDVFLENNKAISNALWEKGIW
                     NALHLWDGESHKARYWRHMVQLYL"
     gene            complement(3377..>3572)
                     /locus_tag="VKQ52_21960"
     CDS             complement(3377..>3572)
                     /locus_tag="VKQ52_21960"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HLZ89940.1"
                     /translation="LHGEQVIYQGEMPYAETFGAVAVGKPLAYLNSLLQLSFALNQGS
                     FAAKYMIASGGEWTVVVRKK"
BASE COUNT          899 a         1168 c          821 g          684 t
ORIGIN      
        1 agtattgcaa ttcttccgtc ccatcaatac ggcccgcctg caataccatc tttccgaccg
       61 cgaaaaagaa gtgctgaact acctcgtaaa aggctatagc tataaaatga tcgccggtta
      121 ctgtcatatc agcctcgaca ccgtaagagg acatatccgg aacatctact ccaagctcca
      181 cgtgaactgc gggcgcgaag ccgtgtccaa ggccctccgc gacaatatcc tgtaaacagc
      241 attatcatgc cattgacgct ccctgaccac ggggctaatt ttacgggaaa acgatctatg
      301 caacaccccc tctctgaatt gagtgcatca ccccggcgca gattcctggg gaagatagcc
      361 gcgggcgccg ccgccctcgc cgtcctcgac ataccattca aactttccgc ggcaccctcg
      421 acacccgccg tcggcccctc cgatcccgat gcctggttca gccagctcaa gggcaaacac
      481 cgccttatac tcgatgtacc ccgccccaat gagctctttc ccttcgcctg gccccgggtc
      541 ttcctgctga caaatgccgc caccggcacc cccgagaaag agaataacgt cgtcgtcgtc
      601 ctccgccacg aaggcatccc atttgccttc gaagatcgta tctgggcaaa atacaaattc
      661 ggcgaagtat tcaaggtcaa tgacccaaaa acaaaagcgc cggccacccg taacccattc
      721 tggaaaccaa tgcccggaga tttcaccatt cccggcatcg gcaaagtgga tatcggcatc
      781 gaccagttgc aaagcagcgg tgtcctgttc tgcgtctgcg acgtggccat gacggtctat
      841 agcgcggcaa tcgccggtga cagcgacccc gcggaagtaa aaaaagactg gatgtccggt
      901 ttgctgccgg gtatccaacc cgttccttcc ggcatctggg ccgtcggtcg cgcccaggaa
      961 catggatgca cctactgcta cgcgggctag ccatgcaacg ccggaaacta caagggaaac
     1021 catatctctt ccttctcgtt attgcctgcg cctgtagccg gggggacaaa aaagctcctg
     1081 tgtgggaagc gccagatacg gccaggatcc cggccacgga tgaaggcagg ctcatccgct
     1141 acggccggga tcttattgcc aacacctccc actacctcgg accaaaggga ataaaagccg
     1201 ctatcaccaa cggaatgaat tgccagaact gccacctgaa cgccggaaca aagccctggg
     1261 gaaataacta cagcgccgta ttttccactt acccgaaata ccgggatcgc agcgcatcgg
     1321 tagaagatat ccaccgccgc gtaaacgatt gcatcgaaag aagcctgaac ggccatcccc
     1381 tcgacacgaa cagcctggaa atgaaagcca tctccgccta tatcaaatgg ctgggccaga
     1441 acgtgccaaa aggagtaaaa cccccggggg ccgggatcag ggatctcccc tggcttacac
     1501 gggccgccga cccggaaaaa ggccgaacgg tctatagaca gaactgccgg cgctgtcacg
     1561 gcacggaagg cggtgggtca cccaaccccg acaactccgg gtacgtatat ccgcccttgt
     1621 ggggtaaaca tagttataac accggcgcag gcctgtaccg catcagccgc ctggcaggct
     1681 atgtaaagga taacatgccg ctgggctgca cccacaacac ccccctgctc accgatgaac
     1741 aggcctggga tgtagccgcc tttatccttt gtcagaaccg gcccgaaaaa gcctttcccg
     1801 gcgactggcc cgacctcacc cggaaaccgg ttgaccatcc cttcggcccc tatgccgaca
     1861 gcttcacagc cagtcaacac aaatacggtc ccttcggccc gatccgccag gtccacccat
     1921 agatccctgt tatcttaatt ttaccaatta ccccctggaa ggggttctcg gctctaaggc
     1981 ttatatttga taagcaaacc ccttctatgg tctggtacga aaatgaggtg caaagactgg
     2041 aacaggaaag agcagcgctt acctacgaac caaaactgct cttctacggc agctcgtcca
     2101 tccggctttg ggaaagcctg aacgaggact tcgccggcta tcagcccgtt aacctgggat
     2161 tcggaggctc gaccctggcc gcctgcgact ggttcttcga tcgcctcgtg gcgccattcc
     2221 accccgaaca gatcatcgtc tacgccggcg acaacgacct cggcgatgga cgccaccccg
     2281 aagagatctt catcttcttc cagcaactcg tcagccgcac ccgcaaacaa ttcggcgaca
     2341 tccccctcgc cttcatctcc atcaaaccca gcatcacccg ctggaacatc gtcgacagca
     2401 tccgctacgc caacaagatc atcgaagacg aaataaaaag acaggccaac cacctgtatt
     2461 ttattgatat ctatcaccgg atgaccgaca actccggcta tccgaaaaga gagttcctgg
     2521 accccgacgg gctccacatc aacgctaaag gctatgcttt atggaaagag atcattttgc
     2581 aatacctatc ggcgaggacc acatgaaaag agaatattac aaatggttca gcaaacccct
     2641 cggaagggac atggaactgc tggtcttcgg ccatgccggt gaccccgtcc tcttcttccc
     2701 aacccgcacc gcccggttct acgactacga ggactggcag gtgatcgaag ccctgcgcaa
     2761 aaagatcgaa accggatcga tccaggtcta ctgcctcgac agcgtcgaca aggaaagttt
     2821 ctacgcgaaa gacctgcatc ccgcccaacg catctgccgc cacctggatt atgaaaaata
     2881 tgtcctcaac gaggtcctcg acctcatcca gcagaaaaat ccccacccca ccgtcattgc
     2941 cgccggctgc agtctcggag cctatcacgc cgccaacctc gccttcaaat acccctggct
     3001 cttccataaa atggtcggcc tcagcggccg ctacgacctg accaccacca tggaatattt
     3061 tgacgatctc ttcgaaggct acgtcgacga aaatatctac tataacatgc ccggccgctt
     3121 catccccaac ctccaggacc agcaattgat cagtctgctg aaagccatgg agatcatctt
     3181 cgtggtcggg gaaaaagacg tcttcctcga aaacaacaaa gccatcagca acgccctctg
     3241 ggaaaaaggc atctggaacg cactgcacct ctgggacggc gagtcccaca aagcccgcta
     3301 ctggcgccat atggtacaac tatacctctg ataccaggcg tcgctgcgcc tccgacgata
     3361 gtcccctatc tctttactat ttcttccgca cgacaaccgt ccactcccca ccgctggcaa
     3421 tcatatattt agctgcaaaa ctcccctggt taagcgcaaa cgaaagctgc agcaggctat
     3481 tcagataagc caatggtttg cccaccgcaa cggcgccaaa cgtttctgca tacggcattt
     3541 ccccctggta gatcacctgc tctccatgca at
//
LOCUS       DATGFG010001158         9246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_757449, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001158 DATGFG010000000
VERSION     DATGFG010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757449"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /gene="nagB"
                     /locus_tag="VKQ52_21965"
     CDS             complement(<1..1378)
                     /gene="nagB"
                     /locus_tag="VKQ52_21965"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF002557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HLZ89941.1"
                     /translation="MSMVDSFEKIPVKIFGSLEEGSRFIGKEIATLIREKAAKGQKAI
                     LGLATGSSPKSLYTELVRLHREEKLSFRNVITFNLDEYYPIDNDALQSYNRFMKEQLF
                     DKVDIDPANCHIPNGQWKKEEIKQHCLQYEQMIEDAGGVDLQILGIGNNGHIGFNEPG
                     SSIYSRTRLVPLDNSTRIANSHDFQNISHVPRLAVTMGISTILKAKRIVLMAWGQMKA
                     PVIQKAVEGNVTEQIPASLLQQHNDCTFVIDEIAASELTRFKSPWLAGDTVWTPAMIR
                     KAVVNMALKLGKPILSLKNSDYNDNGLSDLLVEKGDAYEINLQVFYLLRDSITGWPGG
                     KPNVHLPTHPERSEPFPKRCLIFSPHPDDDIISMGGTFMRLHDQGHDVHVAYQTSGNI
                     AVTDEFVTRFLDFAVGFEELSGIDSRKSSQILDQATRYLKTKKASDIDTPEIRAIKGL
                     IRRCEAKAT"
     gene            complement(1519..2091)
                     /locus_tag="VKQ52_21970"
     CDS             complement(1519..2091)
                     /locus_tag="VKQ52_21970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLZ89942.1"
                     /translation="MNSANQDITLWESFRKGDKEAFATLFREHYETLFRFGSKFTSDT
                     ELLEDCIQELFIELWQAKSATPVLSIKAYLLKSLKYKLLRAFSKKGKMIPLTNDGSDI
                     AFEWSHEMVLISKQDSDEKKQRVLKAMGRLSNRQKEIIYLKYYQNLSYEEVSEIMNIN
                     YQVARNLLYQAIKSLKALLAGTMEIFLLFL"
     gene            2232..3278
                     /locus_tag="VKQ52_21975"
     CDS             2232..3278
                     /locus_tag="VKQ52_21975"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ89943.1"
                     /translation="MSANYTEPEDLLADESFLSWYFKSGAKDQKDWDRWMAEHPEREK
                     LVQQAVALLETVRVREAEIPVQQKQAAEKALLGKLAPLQSRQTPTAPLQSRESPTAEP
                     RSGIYSLYSYRRWMVAAAILLLLGAGVVVVAIRSASDRVVKTAYGQITSRDLPDGSEV
                     TMDANSRLRYSKGWKDGSSREVWIDGEAFFHVRKTPSGSRFIVHTDHFDIIVTGTRFN
                     VVNRNGVENVLLQEGSVILHDKNGKELTMTPGEFVSFDKQQLAKRAVQPDSVTAWKDR
                     KLVFDKTPLRELVGIVNDHYGVHIQLENASLGDSTISAILPNNNLDVLLQALEATSEF
                     TIIRKDDQITIAAR"
     gene            3311..6799
                     /locus_tag="VKQ52_21980"
     CDS             3311..6799
                     /locus_tag="VKQ52_21980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLZ89944.1"
                     /translation="MRKSSFYGCLLVFFLGLILTGIPGLRAQQAPADASRRPLIDVLI
                     ELNKVRGVYFLFSQQSLARTPVNQPVMAPGISVERILGQVLRNTGLRYKRVDDRTFVI
                     LDKRSASRNYAYDSTAAPDYPDTATGSAAPPRMELISGHVIARDGQRLQGVSVSIQHT
                     QKGTTTDLTGTFTLEAAKEDTLVFSFVGYKIRKEVAAGIGAEGILLDASDQPLTEVLV
                     TALGIQKQERSLGYSTEEMDGGIFTGAREVNLGNALVGQVAGLSVAENATGPYGSSRI
                     LIRGNASLSGNNQPLYVVDGIPYDNTTISNAGQYGGLDLGDGLSNLNPDNIESVLVLK
                     GVAASALYGYRGGNGAILIRTKSGSRTHGIGVQVNNNLTVNTVRDEQDYQYVYGQGLS
                     GIKPTTAQMALAAPYYSWGGRLDGSPAVNYLGGSYGYSAAKNNFAHFFRPGVMNQASV
                     ALTGANNKGHFRLGISDLHLAAIVPNSSMKQQGFNFNSTYYVTNRLQMDLTVDYVFEN
                     VNNRASLSDDAGNVIAPPMYLANSFDIRWMKTHTTHPDGTEWLPGTTDLYFENPYYIA
                     YNYQNTTARNRLTGGLTLKYHLLDWLYVQGQVSRDGYLFDVTNIVPSGVEYTRSDGIH
                     GGNLTQYEVDYHELNGSFMIGAHKKMGSDLNVNADFGANRQDNSSTISGIGAVPFSTN
                     RPAGPFLVAGNYNSANILDPIYSSHIRRYRVNSIYGSADLSFRNYLFLNVTARNDWFS
                     TLNINTDHYLYPSVSGSFLFSEVWRLPSWFSLGKLRASYAASSNGTEPYQNALVYGIQ
                     SYSIFGQPLGYIASAPLIPNPDLRPVSIGEKEIGLSAQFLHNRLGLDLTWYNKQTTDD
                     IVRTTVSTTSGYSQHIENIGRIRNAGIEALLTATPVKTPAFSWSLSFNYAVNHNKVLY
                     IGGLPGIVIDGAYPRWGSEVSISNMVGLPYGQIMGYAYRHDAKGNIIYSDGVSNPAPA
                     GEPEPTGVVPLGSTVYKQTGGITNEFHYRGLTLSALVDFKYGAKIYSGTNLLLYYYGL
                     QKATLAGRDGGYVGKGVLENGHPNTIVVPAQQYFQDISAGGTDHIAQEFVYDASFIKL
                     RSVTLTYTVAAAALKKRFIKGLSFSLAGRNLAILMKRTPNIDPESSINNTNGQGLELT
                     GYPTTRSWGFNMNVKF"
     gene            6804..8369
                     /locus_tag="VKQ52_21985"
     CDS             6804..8369
                     /locus_tag="VKQ52_21985"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLZ89945.1"
                     /translation="MKPTSMIYFFFCILLAGSGCKKDFVAINTDPTAITAGQNNFNYL
                     FTNAELVTAGNSDGNGYEDWRNNLIYSGCMIQHLSSTFSYWDGDKYLYNGNYNSAYWD
                     ANYPNSIANIVEVINHAQKDTGSFNLYQMARIFKAFMFQRLTDMYGDCPYFQAGLGYI
                     GGVTTPRYDRQQDIYMDLLHELQSAAAALDPGRPNTIGTADLLYNGDPVAWKGFAYSE
                     MVRVAMRLSKVDPATAAQWVHIAVQGGVITGNAGNAVIHHQNVTGTPDVNGNGLILLG
                     NDPNGYRLHAGFVQFLRSTADPRLSYLATVCANPGVPADKGDTTATRQLGQPGGYDPP
                     NSGGANDLTRAPGWPGDQNAYSVVNRYTFARLDAPTFFLTYGETSLLLAEAAQRGWIG
                     GDAAQYYTDGVRGAMQQLAAQAGAGPGDRQIDSWIAAHPYDPAQGFEQVNDQYWIAGF
                     MDENECFANWRRSGYPALTPVNYPGNVTGGTIPRRFTYPQSEASANGANYTDAVSRLT
                     GGDKMTSRVWWDR"
     gene            8481..>9246
                     /locus_tag="VKQ52_21990"
     CDS             8481..>9246
                     /locus_tag="VKQ52_21990"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLZ89946.1"
                     /translation="MSKNYIGVEDLLSDESFLSWYFKTDTRCIRQWESWMAADPLHRA
                     RAEQAVAFLQSIRYDEPELTVDRLTQAETRLLQQIREAENRSHKRQPLILSGRRWWMA
                     AAAMLFVAAGLYTIREVLSRPLPMVHTAYGEVKENLLPDGSRVVVNANSKLTFSSGWK
                     DGSDREVWLTGEAFFHVTKTPLKSRFIVHVNHFDIIVTGTQFDVVNRQDKANVTLKEG
                     SVILHTEEGKEVKMAPGDFVEYRNDDLRKKTVKPDSV"
BASE COUNT         2095 a         2592 c         2526 g         2033 t
ORIGIN      
        1 aggtggcctt ggcctcacag cggcggatca gccccttgat ggcgcggatc tccggcgtgt
       61 cgatatcgct tgccttcttt gtcttcagat aacgggtcgc ctggtcgagg atctgcgaag
      121 acttcctgct gtcgatgccc gaaagctctt cgaagccgac cgcgaaatcg agaaagcggg
      181 tgacgaactc atccgtcacg gcgatattcc ccgaggtctg gtaggcgaca tgcacgtcgt
      241 gaccctgatc gtgcagccgc ataaaggtcc cgcccatgga gatgatatcg tcatccggat
      301 ggggcgagaa gatcaggcag cgcttgggaa agggttccga ccgctcggga tgcgtcggca
      361 gatgaacatt cggtttgccg ccgggccagc ccgtgatgct gtcccgcagc aggtagaaca
      421 cctggagatt gatctcgtag gcgtctccct tttccaccaa cagatcactg agaccattgt
      481 cattataatc gctgttcttg agactcagga tgggtttgcc caatttgagg gccatgttga
      541 cgacggcctt gcggatcatc gccggtgtcc ataccgtatc ccccgcaagc cagggtgact
      601 tgaagcgcgt gagctcggaa gccgcgatct cgtcgatgac gaacgtacag tcattatgtt
      661 gttgcaggag agaggccggt atctgttcgg tgacattgcc ttccaccgcc ttctgaatga
      721 cgggcgcctt catttggccc cacgccatca ggacgatgcg cttggccttg agaatggtgc
      781 tgatgcccat ggtgacagcc aggcggggaa catgcgagat gttctgaaaa tcgtgggagt
      841 tggcgatacg cgtcgagttg tccagcggaa cgagacgggt gcgggaataa atgctgctgc
      901 cgggctcgtt aaaaccgata tggccgttgt tgccgatgcc gaggatctgc agatccacgc
      961 cgccggcatc ttcgatcatc tgttcgtatt gcagacagtg ttgtttgatc tcttccttct
     1021 tccattgccc gttcgggata tggcagttgg cagggtcgat gtccactttg tcgaacaatt
     1081 gctctttcat gaaccgatta tagctctgca gcgcgtcgtt gtcgatgggg tagtactcgt
     1141 cgaggttgaa cgtgatcacg ttcctgaaac tgagtttttc ttcgcggtgc agccttacca
     1201 gttcggtata caatgacttc ggcgaagaac ccgtggccag gcccaggatg gctttttgcc
     1261 cctttgccgc cttttcacgg ataagcgttg cgatctcctt tccaataaaa cgggagcctt
     1321 cttccaaaga accgaaaatt tttaccggga ttttctcgaa cgagtctacc atgctcatag
     1381 atcagaatgt tttgaataat ttataaaacc ggggcgaatt tacttccaat ttccgatgca
     1441 gaaaaaaaat caggctccgg atcgtacacc agagtgcctg ttacgaaaga aatgtaacca
     1501 tcgttgctgc aaaagctgct aaagaaacaa caaaaagatc tccatcgttc cggcaagaag
     1561 ggccttgagg gatttgatcg cctggtagag gagattacgc gccacctggt aattgatatt
     1621 catgatctcg ctcacctcct cgtaactgag gttctgatag tatttaagat agatgatctc
     1681 tttttgccgg ttggaaagac ggcccatcgc ttttaacacc cgctgttttt tctcatcgct
     1741 atcctgtttg ctgatgagca ccatctcatg actccattca aaggcgatat cgctgccatc
     1801 gttggtgagc gggatcatct tccccttctt gctaaaagct cttaagagct tgtattttag
     1861 tgatttaaga aggtatgcct ttatcgacaa gacgggtgtc gcggacttgg cctgccagag
     1921 ttcgataaac agttcctgga tgcaatcttc cagcagctcg gtatcggaag tgaatttgct
     1981 gccaaaccgg aacagggttt cataatgctc ccggaaaagc gttgcaaagg cttctttgtc
     2041 acctttgcga aaggattccc aaagcgttat gtcctggttt gcgctgttca agggtggttg
     2101 ttgagcgtta ttcaagcagg aaaatacgct aaacctattg tcggtgaaaa aatattttag
     2161 cggatttagg aaaaaagctc aaaaaagcgg gcgatcacca ttttgaaaat tatctttata
     2221 cctagattaa tatgtcagca aactacacag aacccgaaga tctcctggcg gacgaatctt
     2281 ttctatcctg gtattttaaa tcgggtgcaa aagatcaaaa ggactgggat cgctggatgg
     2341 cggagcatcc ggaaagggaa aaattggtac aacaggcggt agccctgctg gagacggtgc
     2401 gggtgcgtga ggcggagatc ccggtgcaac agaaacaggc cgcggagaag gcattgcttg
     2461 gcaaactggc gccgctccaa tcccggcaga ccccaactgc gccactccaa tcccgggaaa
     2521 gcccgactgc cgaaccccgc tcgggtattt acagccttta tagttatcgc cgctggatgg
     2581 tggccgccgc catactcctc cttctcggcg cgggtgtcgt cgttgtcgcc atccgttcgg
     2641 ccagtgacag ggtagtgaag acggcctatg ggcagataac gagtcgtgac ctgcccgacg
     2701 gtagcgaggt aacgatggat gccaacagcc ggctgcggta ttccaaagga tggaaggacg
     2761 gctccagccg ggaagtatgg atcgatggcg aagccttctt tcatgtccgg aagacccctt
     2821 ccggcagccg cttcattgtt cataccgatc atttcgatat catcgtaacc ggcacacgct
     2881 tcaatgtcgt caatcgcaat ggcgtagaga acgtgttgct tcaggaagga agcgttatcc
     2941 ttcacgataa gaacggtaag gagttgacaa tgacccccgg ggaatttgtg agcttcgata
     3001 aacagcaact ggcaaaaaga gccgtccagc ccgacagcgt caccgcctgg aaagacagga
     3061 agttggtgtt cgacaagact ccgctgcggg aactggtggg tatcgtcaac gaccattacg
     3121 gagtccatat acaactggag aatgcttctc tcggggatag caccatctcg gccattttgc
     3181 ctaacaacaa cctggatgtc ctgttgcagg ccctggaagc gacctcggag ttcaccatca
     3241 tccgcaagga cgatcagatc acgatcgccg cccgttgagg acggaactaa ttgataccaa
     3301 acactgcacc atgagaaaat caagctttta cggctgcctg cttgtatttt ttctgggcct
     3361 tatcctcacc ggtataccag ggctccgggc acagcaggca ccggccgacg cttcccgccg
     3421 tcccctgatc gatgtactga tcgaactgaa caaggtcagg ggcgtatatt ttcttttctc
     3481 ccagcaatcg cttgccagga cgccggtgaa ccaaccggta atggcgcctg gcatctccgt
     3541 cgaaaggatc cttggtcagg tgttaaggaa caccggtctt cgttacaaaa gggtggacga
     3601 ccgaaccttt gtcatccttg acaagcgctc ggcctcccga aactatgctt acgattcgac
     3661 ggcagcgccg gattacccgg atacggcaac gggctctgcc gccccgccac gcatggaact
     3721 gatctccggc catgtcatcg cccgcgacgg gcagcgtctc cagggggtga gcgtctccat
     3781 tcaacatacg caaaaaggga ccaccaccga ccttacaggg accttcacgc tggaagcggc
     3841 aaaagaggac accctcgttt tttcctttgt cggctataaa ataaggaagg aggtggcagc
     3901 cgggatcggc gccgaaggca tcctgctcga cgcctccgac caacccctga cggaggtgct
     3961 ggtcacggcc ctcggcattc aaaaacagga acgctcgctg gggtattcca ccgaggaaat
     4021 ggacggaggc atcttcaccg gggcaaggga ggtcaacctg gggaatgcac tcgtcggcca
     4081 ggttgccgga cttagcgtag cggagaacgc gacgggaccg tatggaagca gccgcatcct
     4141 tatccgcggg aatgcttctc tcagcggcaa caaccagccg ctctatgtcg tggacgggat
     4201 cccttatgac aacactacca tcagcaatgc cgggcagtat ggcggcctgg atcttggcga
     4261 cggcctgtcc aatctgaacc ctgacaatat cgaatccgtt ctcgtactga aaggcgtggc
     4321 ggcctctgcc ctgtatggtt atcgcggtgg taacggcgcc atccttatcc ggacaaaatc
     4381 cggttcccgc acgcatggta tcggggtgca ggtgaacaat aacctgacgg tgaacacggt
     4441 gagggacgaa caggactatc agtatgtcta tggtcaaggg ctctcgggga tcaagcccac
     4501 gaccgcccag atggcgctgg cggcgccata ttatagctgg ggcggccgcc tggatggcag
     4561 ccccgccgtg aactatcttg ggggcagtta tgggtacagc gcggcaaaga acaactttgc
     4621 gcacttcttc cggccggggg ttatgaacca ggcctcggtc gcgcttacgg gcgccaataa
     4681 taaaggccat ttccggttag ggatatccga tctgcatttg gctgccattg tgcccaattc
     4741 ctccatgaaa caacaggggt tcaatttcaa ttccacctat tatgtgacca accgcctgca
     4801 aatggatctg acggtggact atgtgttcga gaacgtgaac aaccgcgcct cgctttccga
     4861 cgatgccggc aatgtcatcg cgccgccgat gtacctggcc aattccttcg acatccgctg
     4921 gatgaagaca cataccaccc atccggatgg aacggaatgg ctgcccggca ctaccgacct
     4981 ttatttcgag aacccctatt atatcgccta taattaccag aacaccaccg cgcggaaccg
     5041 gctaacgggg gggcttacct taaaatacca cctgctggac tggctgtatg tccagggaca
     5101 ggtatcccgc gacggctacc tgttcgatgt gacgaatatt gttccatccg gagtggaata
     5161 tacccgcagt gacggcatcc acggcggcaa tcttacccag tacgaggtgg attatcatga
     5221 actgaacggg agttttatga tcggagcgca taagaagatg ggatccgacc tgaatgtcaa
     5281 tgccgatttt ggcgccaacc gccaggataa ttcctctacg atctccggta tcggcgccgt
     5341 ccccttttcg accaaccggc ccgcgggacc cttcctcgtc gcggggaatt acaattccgc
     5401 caatatcctc gaccccatct atagctccca tattcgccgt tatcgtgtga acagcatcta
     5461 tggcagcgcc gacctgagct ttcggaatta tctcttcctg aacgtcacgg cccgcaacga
     5521 ttggttctcg acgctgaaca tcaataccga tcactatctc tatcccagcg tctccggcag
     5581 cttccttttc tccgaagtct ggcggctgcc atcctggttc agtcttggga aactacgggc
     5641 ttcctacgca gcgtcttcca atggtacgga gccctatcag aatgccctgg tatatggcat
     5701 ccagagctat tctatctttg gtcagccgct cggctatatc gcctcggccc cgcttatccc
     5761 taaccccgat ctgcgcccgg tgagcatcgg ggagaaagag atcgggctaa gcgcgcagtt
     5821 ccttcacaac cggcttggcc tcgacctcac ctggtacaac aaacagacga ccgatgatat
     5881 cgtccgaacg acggtgagca cgacttccgg ctacagccag catatcgaga atatcggccg
     5941 catccggaat gccggcatcg aagcgctact caccgcaaca ccggtgaaga cgcctgcttt
     6001 cagctggagt ctctccttta attatgccgt caatcataac aaagtgttgt atatcggcgg
     6061 cctgccgggg atcgtgatcg atggcgctta tccccgctgg ggcagcgagg tcagcatcag
     6121 caatatggtc gggctgccct atggccagat catggggtat gcctatcgcc atgatgcaaa
     6181 gggcaatatt atttatagcg atggggtcag caatcccgcg cccgcgggcg agccggagcc
     6241 cacaggcgtg gtgccgctcg ggtccaccgt ttataaacag acgggcggga tcaccaacga
     6301 attccattac cgggggctca cgctttccgc gctggtggat ttcaaatatg gcgcgaagat
     6361 ctattccggc accaacctgc tcctttatta ttatggactg caaaaggcga cattagccgg
     6421 cagggatggc gggtatgtcg ggaaaggcgt gctggagaat ggccatccca atacgatcgt
     6481 cgtccctgcg cagcaatact tccaggatat ctccgccggc ggaacggacc atatcgccca
     6541 ggagttcgtc tatgatgcga gttttatcaa actgcggtcg gtgacgctca cctataccgt
     6601 ggcggccgca gcattgaaaa agcgatttat aaaagggttg agtttttcgc tggcgggccg
     6661 caacctggcc atcctgatga agcgcacccc aaatatcgac cccgagtcca gtatcaataa
     6721 taccaacgga caggggctgg agctgaccgg ctatcccacg acgaggagct ggggattcaa
     6781 tatgaatgta aaattttaac cggatgaagc cgacgagcat gatatacttt ttcttttgca
     6841 tcctgctggc cggcagcggg tgtaagaaag actttgtggc gatcaacacc gatcccacgg
     6901 cgatcaccgc cggccagaac aattttaatt atctcttcac caatgcggag ctggtcactg
     6961 ccgggaattc ggatggcaat gggtatgagg actggcggaa caatcttatc tactcggggt
     7021 gcatgatcca gcacctgtcc tctacttttt cttactggga cggcgacaaa tacctttata
     7081 acggaaatta caactccgcc tactgggatg ccaattaccc caacagcatc gccaatatcg
     7141 tcgaggtgat caaccacgcg cagaaggaca ccggttcttt taacctgtac cagatggcgc
     7201 gcatcttcaa ggcgtttatg tttcagcggc tgaccgatat gtatggcgac tgtccctatt
     7261 tccaggccgg acttgggtat atcggcggtg ttacgacccc gcgctacgac cggcagcagg
     7321 atatctatat ggacctcctg cacgagttgc agagtgcggc cgcggcgctc gatcccggca
     7381 ggcccaatac gatcggaacc gccgatctgt tatataatgg tgaccccgtc gcctggaaag
     7441 gctttgccta ttcggaaatg gtccgggtgg cgatgcggct ttccaaagtt gacccggcta
     7501 ccgccgctca gtgggtgcac atcgccgtgc agggtggggt gataacaggg aatgcgggta
     7561 acgccgttat ccatcatcag aatgtcaccg gtacgccgga tgtcaacggc aacgggctca
     7621 tcctgctcgg aaatgacccc aacggctacc gcctccatgc cggctttgtc caattcctgc
     7681 gatcgacggc cgacccccgg ctttcctacc tggcaaccgt atgcgcaaac cccggcgttc
     7741 cggccgacaa gggggatacc acggctaccc gtcaactggg tcagcccggg ggatatgacc
     7801 caccgaacag cggcggcgcc aacgatctga cccgcgcgcc cggctggccc ggcgatcaga
     7861 acgcctactc cgtcgtcaac cgctacacct ttgcccggct ggatgcgcct actttctttc
     7921 tcacctatgg tgagacttcc ctgttgctgg cggaagcggc ccagcggggc tggatcggcg
     7981 gcgatgcggc gcagtattat accgatgggg tacgcggcgc catgcagcaa ctcgccgcgc
     8041 aggccggggc cggaccgggc gaccgccaga tcgatagctg gatcgccgcg catccctatg
     8101 acccggcaca ggggttcgaa caggtaaatg accaatactg gatcgccggg ttcatggatg
     8161 agaacgaatg ttttgccaat tggcggcgaa gcggctaccc ggcgctgacg cccgtaaact
     8221 atcccgggaa tgtcaccggc ggcacgatcc cccgccgctt cacctacccg caaagcgagg
     8281 cctccgccaa tggcgccaac tacaccgatg ccgtctcccg gctgaccggt ggggataaga
     8341 tgacctcccg ggtctggtgg gaccgctaac ccaatcccgg tgggaccgct aacctgctcc
     8401 ggcggcctca aaaaaaattt acaaaaagat cattacaagg cgttggaccc tgacactctt
     8461 tcagtcaggg gacaactgtt atgtcaaaga actatatcgg ggtagaagat cttttatcgg
     8521 atgaatcctt tctttcctgg tattttaaaa cggatacccg ctgcatccgg cagtgggaat
     8581 cctggatggc tgcagaccct cttcaccgcg cgcgcgcgga gcaggccgtt gcttttctcc
     8641 aatccatccg ctatgatgaa ccggagttga ccgtggatcg gttgacacag gccgagacca
     8701 ggctattgca acagatccgc gaagcggaga accggagcca taaacgccaa cctcttattc
     8761 tatccggccg gcgctggtgg atggccgccg cggctatgct cttcgtggca gccggactct
     8821 atactatccg ggaggtcttg tcccgcccgc tgccgatggt gcataccgcc tatggcgaag
     8881 taaaagagaa ccttctcccc gacggcagcc gggtcgttgt caatgccaac agtaaactta
     8941 ccttctcttc cggctggaag gatggcagtg accgggaggt ctggctcacg ggtgaagcct
     9001 tctttcacgt caccaaaacg cccctgaaaa gccggttcat cgtacacgtc aatcacttcg
     9061 atattatcgt cacgggcacc cagttcgatg tcgtcaaccg gcaggacaag gccaatgtca
     9121 cgctcaagga aggcagtgtc atcctgcata cggaggaagg aaaggaagta aagatggcgc
     9181 caggcgattt tgtggaatac cgcaacgacg atctccggaa aaagaccgtt aagccggaca
     9241 gtgtca
//
LOCUS       DATGFG010001159         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_304755, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001159 DATGFG010000000
VERSION     DATGFG010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304755"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VKQ52_21995"
     CDS             complement(<1..800)
                     /locus_tag="VKQ52_21995"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLZ89947.1"
                     /translation="MVQKLIEWSLRNRLIVLVLAAALLGWGFYSVRQNPIDAIPDLSE
                     NQVIVFTEWPGRSPQIMEDQVTYPLVSNLQGIPKVKNVRGTSMFGMSFVYIIFDDAVD
                     VYWARSRVLERLSYAQRLLPADVVPTLGPDGTGVGHVFWYTLDAKGMDLGDQRALQDW
                     YVKLALQTVPGVAEVASFGGFEKKYQLVVHPVKLQYYQLSMMDVARAVKANNNDVGGR
                     KFDRGDMSYIIRGLGYVKSVDDLENIAVGADKGVPIRVKDIGSVQLGG"
     gene            complement(934..1350)
                     /locus_tag="VKQ52_22000"
     CDS             complement(934..1350)
                     /locus_tag="VKQ52_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89948.1"
                     /translation="MKKVLVFILAVIYLSTSIGATVHLHYCMGRLVSWGLADRSGKSC
                     EFCGMQKMGPSGESVVGMKNCCHEESRQIKNDKDQKTGQEFFQMIKAAPAIADLPQPV
                     WVDAAVVSPVLSQPVSHGPPPAGLFPLFLRNRNFRI"
     gene            complement(1441..>2090)
                     /locus_tag="VKQ52_22005"
     CDS             complement(1441..>2090)
                     /locus_tag="VKQ52_22005"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ89949.1"
                     /translation="AAFFRSFTDNEGIIRTNHAGTTALANFAMAAGVKRFVFTSTGNV
                     YGSGYRHPAREDDPVNSNDPRAYSSSKIAAEHELLSLYKTNGFDVRVLRLGFVYGDKD
                     PHIEEIIPLLKKMKRHSGSRMHMVHYLDVAQALMLLLHTDGLNGETFNLADDAPITLY
                     ELADSVGRARDAFDPEEGPLSDPFEGILDVSKLRNRTGFRPLVPCYYVARDLDIL"
BASE COUNT          516 a          643 c          474 g          457 t
ORIGIN      
        1 tcgccgccga gctgcacgga ccctatatcc tttacgcgaa tgggaacgcc cttgtcagcg
       61 ccaacggcga tattctccag atcgtccacg ctcttcacgt aacccaatcc ccggatgata
      121 taggacatat cgccccggtc gaacttcctt cctcctacgt cgttgttatt cgccttcact
      181 gcccgcgcaa cgtccatcat cgacagctgg tagtactgta gcttcaccgg gtggacgacc
      241 aactggtact tcttctcaaa accgccgaaa gaagccactt ccgccacgcc cggcaccgtc
      301 tgcaacgcca gcttcacata ccagtcctgc aaggcccgct gatcgcccag atccatcccc
      361 ttcgcgtcca gcgtgtacca aaagacatgc cccacccccg tcccatccgg gcccagcgtg
      421 ggcaccacat ccgcaggcaa cagccgctgc gcataactca gccgttcgag cacccggctc
      481 cgcgcccaat acacatccac ggcatcgtca aaaatgatat acacgaaact catcccgaac
      541 atcgaggttc cccgcacatt cttcaccttt ggaatgcctt gcaggttgct caccagcgga
      601 taggtcacct ggtcctccat tatctgcggg ctccggcccg gccattccgt aaacacgatc
      661 acctggttct ccgacagatc ggggatcgcg tcgattgggt tctgccggac ggagtaaaat
      721 ccccagccca gtagggccgc ggccagcacc agcacgatca gccggttgcg tagcgaccat
      781 tcgattaact tttgtaccat ggaattaatt gcgagcctgc cttttcgtca aagggaaaag
      841 cgctcaaatg gaaccattaa gaaatagtga gaacaagcgt tccgggtcac cggaaatgct
      901 aaaagcccaa caggacgtca ggcatgcaca gcatcaaata cggaaattac gattgcgcag
      961 gaatagcgga aacaaaccag ccggcggcgg accgtgcgaa acaggctgag acaaaacggg
     1021 tgaaaccacc gccgcatcca cccacaccgg ctgcggaaga tccgctatcg ctggcgccgc
     1081 ctttatcatc tggaaaaact cctgtcccgt cttttggtcc ttgtcgttct tgatctgcct
     1141 gctttcttcg tggcagcagt tcttcatccc aacgacactt tcccccgagg gccccatctt
     1201 ctgcatcccg caaaactcac aactcttccc cgagcgatcc gccagccccc aggacaccag
     1261 gcggcccatg caataatgca aatgaaccgt cgcccctatc gaagtggaga gatagataac
     1321 cgctaatatg aaaacgagga cttttttcat gggaatctgc cccaaagcta tgcactttcc
     1381 gcaaatacct gttatacaat ttccgtaatt gtttgtaaaa tattggggaa taggtatgta
     1441 ctacaaaatg tccagatccc gagccacata ataacatggc acaagaggcc tgaagccggt
     1501 cctgttcctg agtttcgaaa catccaggat gccttcgaac ggatcgctta gcggcccttc
     1561 ttccggatca aacgcatccc ttgcccggcc aacagaatcg gccaattcat acaatgtgat
     1621 tggcgcatca tctgccaggt tgaaggtctc accattcagc ccatcggtat gaagcagtaa
     1681 catcagcgcc tgggctacat ccaggtaatg gaccatatgc atccttgaac cggaatggcg
     1741 tttcattttt ttcagcagag ggatgatctc ttcgatatga gggtctttat ccccatacac
     1801 aaatcctaaa cgcaacaccc gcacatcaaa gccgttggtt ttataaaggg aaagaagttc
     1861 gtgctccgcg gcgatcttgc tggaggagta tgccctggga tcattactat ttaccggatc
     1921 gtcttctctg gcaggatgcc ggtaacccga tccatataca ttacctgtgc tggtgaaaac
     1981 gaatcgtttc acccctgctg ccatagcgaa attggccagc gccgtggttc ctgcatgatt
     2041 tgtcctgata atgccttcat tatcggtaaa actgcgaaag aaggcagcaa
//
LOCUS       DATGFG010001160         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_772331, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001160 DATGFG010000000
VERSION     DATGFG010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772331"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="VKQ52_22010"
     CDS             <1..1637
                     /locus_tag="VKQ52_22010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ89950.1"
                     /translation="MQQTGGSYGLFNSFHSIGGQLGKFNYYSFVQYQAMQGWRPNSDY
                     RQISAFGKLSYQASSRLRLGLEYTLFRNRIHMPGGFTDAQFAYKSDTSYRARNWLESP
                     WNIIAAHLDYSLSANTSLHITSAWLFSSRNLVWKNEDGGPGTPDSISTVTLQYVNREV
                     QREKMNSLTTEIRLLSNYRLAGMNNTLAAGIRVFAGKFKRQGGGLGTTGSDFDLSLLD
                     PHYEYDLDFHTTNVALFAENIFRVTPRFSVTPGVRYEWLRSAAKGYITDEGTEVTTDS
                     ARNRYIPLFGIGLQYKTTPTTNVYANISQAYKPITYDQVTPYAVASRIDPNLKDARGY
                     NADLGWRGSFGRYLNFDVNGFYLAYDNRIGLVQLTDANGNPYTYRTNVANSVHKGVEA
                     YLEFSPTRLAKTVPRFGNWSIFSSFAYVHATYTTGGYKGNFVEYAPQTILRSGITWCY
                     RPFSTTFLVSRTARSFGDANNTVSSSDAIVGIIPAYTVLDWSATLRLKGGWNVKAGMN
                     NLTDKRYFTLRTDEYPGPGIIPAIGRAAYLSFGVYF"
     gene            complement(1670..>3112)
                     /locus_tag="VKQ52_22015"
     CDS             complement(1670..>3112)
                     /locus_tag="VKQ52_22015"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HLZ89951.1"
                     /translation="SIDQLEQTQTNPGSIYADTNSINKTTSAFRQYSFDPSLNYKHGF
                     KNKDQQLEIGMDGSFAHNTTTAGNDQILQPEDSLIYGTRNNNPATESEYEVKLDYTQP
                     LYKDVSLGAGGKFSGHDISSTGDALVWNPYLSDYLYNSSLSNDLNYHQKVYAAYAELN
                     FPVTSSIDARIGGRYERTQVNSFYANAYQTIHNGYNTLIPSIFLMKKIDETQTFKVNF
                     TIRINRPEYSELNPFINTSDPKNISTGNPNLNPEIWDRFEAVYNKDLGKTGSFMITLF
                     YRQSNGDIQPFIVNYPSIQVGDTAYTNVAVTTRKNIGIEENAGTNLFFDLHVTEKLNV
                     RSNMTFFFRHTINQVDPGYNSNTTIYRFNINAAHQFSGNFAAEFFGNFNSRHHEAQGK
                     YPSFISYSLALRKLFWNKKGSLALTANNFFSKYVDQQTDLYGPGFVSSGLRRIIYRSV
                     GLNFTWKFGRLVVRKERSEDNTLDLNAAQP"
BASE COUNT          712 a          772 c          802 g          826 t
ORIGIN      
        1 ccatgcaaca gaccgggggt agttatggct tgttcaattc ctttcattcc ataggcggac
       61 agttggggaa attcaactat tacagctttg ttcaatacca ggcgatgcaa ggctggcgcc
      121 ctaattccga ctaccggcag atcagcgcct tcggcaagct gagctaccag gccagctccc
      181 gccttcggct tggattggaa tataccctgt tccgcaaccg gatccatatg cccggcggct
      241 tcaccgatgc gcaattcgct tacaagagcg acacgtccta ccgggcaagg aattggctgg
      301 agagcccctg gaacatcatt gcggcccacc tggactattc cctttcggcc aacacctccc
      361 tgcatattac gtcggcctgg ctgttcagct cccgtaacct cgtttggaag aacgaggatg
      421 gcgggcccgg cacgcccgat agcatcagta ctgtgacctt acaatacgtc aaccgggaag
      481 tgcagcggga gaaaatgaac agcctcacca ccgagatccg cctgctctcg aactatcgct
      541 tggcggggat gaataatacg ctggccgccg gtatccgggt ctttgccggc aagttcaaac
      601 ggcagggggg tggcctgggg accaccggca gcgatttcga ccttagcctt ctcgatccgc
      661 attatgaata tgacctcgac ttccacacca ccaacgtcgc gctttttgct gaaaatattt
      721 ttcgcgttac cccgcgtttc tccgtcactc cgggcgttcg gtacgagtgg ctgcgttccg
      781 ccgcgaaagg ttatatcacc gacgagggga cggaggtgac gacagacagc gcccgcaacc
      841 ggtacatccc tttattcggc attgggctgc agtacaagac cacacctacg actaacgtct
      901 atgccaatat ctcccaggca tacaaaccca ttacgtacga ccaggtcacc ccttatgccg
      961 ttgcttcgcg catcgatcct aacctgaaag atgcccgggg gtataacgcc gatctgggat
     1021 ggcgtggttc cttcggcagg tatctcaatt ttgatgttaa tggcttttat ctggcttatg
     1081 acaacagaat aggacttgtt caactcaccg atgcaaatgg caatccatac acctaccgta
     1141 cgaatgtggc caatagcgta cataaaggag tagaagccta tctcgagttc agtcctaccc
     1201 ggctggcaaa gaccgtaccc cgatttggaa attggagcat tttcagttcc ttcgcctatg
     1261 tccatgcgac ctataccacc ggcgggtata aaggcaattt tgtagaatat gcaccgcaaa
     1321 cgatccttcg ttcgggaatc acgtggtgct accggccgtt ttctaccacg tttctggtga
     1381 gtcgaacggc gcggtccttc ggggacgcca ataacaccgt ctccagcagc gatgccatcg
     1441 tcggaatcat tcccgcttat accgtattgg attggtcggc tactttgcga ttgaagggtg
     1501 gatggaatgt caaggccggg atgaacaatc tcacggataa acgatacttc acgctccgga
     1561 cagatgaata tccgggaccg ggtatcattc cggcgatcgg gagggctgct tacctatcct
     1621 ttggagtgta tttttgatgg tatttgtatc gtcatgacgt ttctcctggc tacggttggg
     1681 cagcattaag atcaagggta ttgtcctctg atctttcctt tctcactacc agtcttccaa
     1741 atttccaggt gaagttgagg ccgaccgacc ggtaaattat cctgcggaga ccactgctca
     1801 caaagccggg gccataaagg tcggtttgct gatccacata tttgctgaaa aagttattgg
     1861 ctgtgagggc aaggcttcct ttcttattcc aaaacaactt cctgagcgcc aaactatagg
     1921 aaataaagga gggatatttt ccttgcgctt cgtgatggcg ggaattgaaa tttccgaaga
     1981 attccgccgc aaaatttccc gaaaattgat gcgcggcgtt gatgttgaaa cgatagatag
     2041 tggtgttgga attgtatccg gggtctacct ggttgatggt atgcctgaag aagaaggtca
     2101 tgttcgaacg tacattgagt ttttcagtta catggagatc gaagaataag ttggtgccgg
     2161 cgttttcttc gattccgatg ttcttccggg ttgtcacggc tacgttggta taggcggtgt
     2221 caccgacctg gatggacggg taattgacga tgaaaggctg tatatcgccg ttgctttgtc
     2281 ggtaaaacag ggtgatcata aacgatcctg tcttgccaag gtccttgtta tatacggctt
     2341 caaaacgatc ccagatctcc ggattcagat tagggttacc cgttgagata tttttaggat
     2401 cactcgtgtt gataaagggg ttcagctccg agtattcggg acggttgatc cggatcgtga
     2461 aattcacctt gaatgtttgt gtctcatcga tctttttcat caaaaaaatg gaaggaatca
     2521 atgtgttata gccattatgg atggtctgat aagcgttggc gtaaaacgag ttgacctggg
     2581 tccgttcata ccgtccgccg attctggcat cgatcgagct ggtgacgggg aaattcaatt
     2641 cggcgtatgc cgcatacacc ttttgatggt agttcaagtc gttggatagg gaggaattgt
     2701 ataaataatc agaaaggtaa gggttccaga ccaacgcatc gccggtactg gaaatatcat
     2761 ggccactgaa ttttcctcct gctccgagac tgacgtcctt gtacagaggc tgcgtgtaat
     2821 ccagcttcac ctcatactcg ctttctgttg cggggttatt gtttctcgtg ccgtagatca
     2881 gagaatcttc cggctgtaag atttgatcat tgccggccgt ggttgtgtta tgcgcaaagc
     2941 ttccgtccat tccgatctcc agttgctgat ccttgttttt aaaaccgtgc ttataattga
     3001 gactcggatc gaaactgtat tgccggaagg cactggtcgt cttgttgatc gagttcgtat
     3061 ccgcatagat gctgcccgga ttggtttgcg tctgctccaa ttggtcgatg ct
//
LOCUS       DATGFG010001161         6237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1098058, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001161 DATGFG010000000
VERSION     DATGFG010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098058"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..>6237
                     /locus_tag="VKQ52_22020"
     CDS             164..>6237
                     /locus_tag="VKQ52_22020"
                     /inference="COORDINATES: protein motif:HMM:NF015501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase-like domain-containing protein"
                     /protein_id="HLZ89952.1"
                     /translation="MEKMTSPAASAPAVYKVYGTVRDQYQQHIVGALIGAFDKDIRSE
                     QDLGRAKTDQNGYYEIDYVGQQFALTDKNAADVFVRLYNANGGIRYESPITYNAPQQL
                     ELDIDLSGQAFEGPSDFEQMQAAIIPYTGDLDLSTLSENDKLHDITFLINKTGLPQDK
                     IEDFGMAWRFGSLTKVEPAIYYGLLRENIPGNTLSNSLSHLSSNVYEPQLNYILDGMM
                     HENIDVLMSGIKKAIADNIIAYRFTASLSRIREQLTAAMNAYAQSHPVTGDPSQIFTK
                     LQIAGLSKDDSDQFMTLYSQHLGTIDQLFTNLQANPTLGPSTDKVNAVFQLSGLTNGN
                     MDLTRHLVESQSISRPDDLKKLAGYDSHDWQSILEQIGQTGGGRTTPGERLEAGFTSK
                     FPTAAFTARLQKDTQSPITHAGHIAGFLNAHPEFDLLHTRVHQFANAHPEFFSQPSPF
                     THNTAAPGGTISPDGTPPAALPDQAALTDQLKKIQRVFKIAPTYQATAALMQDNIHSA
                     VQVYRMGKENFVRKYGNTVGQKAAKDIFQKATQAYSQSITLAANIKSLSEASHLNVFP
                     DYKKLLTPLTAEIPDLQTLFGNGDYCECDDCNAVDGAAAYLADILHYLDERPSKQVGI
                     SVKDWLLLRRPDISDIDLDCDNTNTEIPYIDIACELMEDYINSPTIQIAIPAPVKGKI
                     DPGTVTSIQTKFTAIGMKNISDSLTTNAIVSESYSSTWYNGTTKVTTTNWIIRDTLVV
                     LKVTDASGGKFNVKLLHQTLLSSDEVSANPEYINLPVYQDNAALSMKGILNNAQRPFS
                     LPFDLFETEGELYLLKLGINKYDLIKTFQKEHDISGPPSSNDLAYAYAWLDVTPAERT
                     LIFQADTVNQTHYWGSIASGTTVGIDDFENASGLAYSDILNLLGLTLVNPGQVIVIQH
                     DAPPSCDTTKQRLINMSPGAFDIMHRFLRLWRKTSLVMSDLDAIVRSTAVGAGKIDPA
                     FAVAFQPFLLLQQAWSLSTPQLLSFYQNIDTTGSDNLYLQTFQNKSVTNPVNPDFSIT
                     NVTAGAMAISPLHQAVITASTGISSTDLTTLIALTDGKLSLANLSYFYRVYLLSQALS
                     ISVADLLHAVDLFNINPFSGPALTASFRDKFTFLTGAGFSLDDLNYVLRQQDTPDNQY
                     IASNKQIAANLATLQTALLSATAATTPAPDPKGALLAKWLADPLLAWDPALVTKLFDI
                     LNTSDDGQYAQKITNDTGFLNDLRVQYNAKNVSANLSALPAFSPADLDIYQSYSTQIS
                     YDSVGLQLIWRGCMSTADQTALNGLASATTDFKNAVTNLFNQTQLTSSASANVFIHSA
                     ADITTLSAIPFTSVAQRFAFFLNAISPVYLTLMQQQAIASQITTWFSVARQITAQLMT
                     SIPGIFTDYTATPFITRQLDLTAVNYPNQFNRYLQLQKICFLAARLQLSVTDIQWQLA
                     NAAAAGSLNYLSLPLASLTGPVTTFPQFINLIDILKFSQHHPVVITDPTVTPPITLSV
                     YDILTDAINAKPIAQIEKDLVTLTGWNADNLKKLVEAPNYLNLTSPSDFTSPAILSRL
                     DACFSVLSLLNIGADDAIAWSKDSLSYDDAVKIVQTLKTGYSDADWVPVTQPLQDKLR
                     EKKRDALIAWLLANPGSQSWVTDTDLYSYFLLDVEMCACQPTSRIVQATNSVQLFVQR
                     CFLALELNIAVDTTVDSNWTQWQWMKFFRLWQANYEVFLWPENWTEPELLPVQSSYFT
                     DLQTALSQNEVTESNVEDAFMAYLQSLDGVARLEVKGMWYDDPNKQFYVVARTYGGDP
                     KIYYFRQLSIVDGIWTPWQKIDLDIKSDHIIPVVYNNRFYIFWAVFGEKSDTPTSLNV
                     PDIKESSFPVPQPTKYWQIQMAYSEYRNGKWLAKKISADDDTGIIQAQENDGTTPIYP
                     DKSAFLFTALDMPQIDLKTLLEQYKNLPATGGKKESFAQFLLDTVAQALSQNGDLIIN
                     CYYTKQESSGPYYEYKGSFDLDPCRGYPVVVTKAVQIHPWIFDNSALSSMLDDEDSTN
                     DLALSSVP"
BASE COUNT         1553 a         1973 c         1382 g         1329 t
ORIGIN      
        1 ctgagtgaaa tgctccattg cactgccgga caccatcgat tatttaaggt gaacacctga
       61 tcagcagcta ttcgtctgaa ccagtttaca cggtcaatga cctgactgaa tttcatgccc
      121 tgacgcgaac tcattttaaa gtgctcaatt aaaaactctc tccatggaaa aaatgacttc
      181 ccccgccgct tcagcacctg ccgtctataa agtgtatggt accgtaaggg accaatacca
      241 gcaacacatt gtcggtgcgc tcatcggcgc tttcgacaag gatatccgaa gcgaacagga
      301 cctgggacgg gcaaagaccg atcagaacgg atattatgag attgactatg taggtcagca
      361 attcgccctc accgataaaa atgccgcaga cgtattcgtc cgcttgtata atgcgaacgg
      421 cggcatccgg tatgagtcgc caatcactta taacgcaccg caacagcttg agctggatat
      481 cgatctgagt ggacaggctt tcgaaggccc ctccgatttc gaacagatgc aggctgccat
      541 cattccatac accggcgacc tggacctttc cacgctttct gaaaatgata agctgcacga
      601 tatcaccttc ctgatcaata agaccggcct tccccaggat aaaatagaag atttcggcat
      661 ggcctggcgc ttcggcagcc tgaccaaggt agagccggcc atatactatg ggctgcttcg
      721 cgaaaatata ccgggcaaca cattgagcaa ttctctctcc cacttgtcca gcaatgttta
      781 cgaaccccag ctcaactaca tcctggacgg catgatgcat gaaaatatcg atgtgttgat
      841 gagcggcatc aaaaaagcca ttgccgacaa tatcatcgcc taccggttta cggcatccct
      901 gagccggatc agggaacagc tcacggccgc tatgaatgcc tatgcccaat cccatcctgt
      961 cacaggcgat cccagccaga tcttcacgaa attgcagatc gcgggactca gtaaggatga
     1021 ttcagatcaa ttcatgacct tgtattccca gcaccttggc acgatcgatc aattattcac
     1081 taacctgcaa gccaacccaa ccctcggccc ttccaccgat aaggtgaatg ccgtctttca
     1141 actgagcgga cttacgaacg gtaacatgga cctgacccgg cacctggttg aatcgcagtc
     1201 gatctcccgg cccgatgacc tcaaaaaact tgcgggatac gacagtcatg actggcagtc
     1261 catcctggaa cagatcggcc agacaggcgg agggagaacc actccgggtg aaagactgga
     1321 ggccggattt accagcaaat ttccgacagc cgcctttacc gcccgcctgc agaaggacac
     1381 ccagtcgccc attacccacg ccggacatat cgcgggcttt ctgaatgcac acccggaatt
     1441 cgacctgctg cacacaaggg tccaccaatt cgccaacgca catcctgaat tcttttcaca
     1501 gccatccccc ttcactcaca atacggcagc acccggcggc acaatttctc cggacggcac
     1561 accgcccgct gctttacctg atcaggctgc ccttaccgac caattgaaga aaattcaaag
     1621 ggtattcaaa atcgcaccta cctaccaggc cacagccgcc ctcatgcagg ataatatcca
     1681 ttccgccgtc caggtctata ggatgggcaa ggagaatttt gtcaggaaat acgggaatac
     1741 agtaggacag aaggctgcaa aagatatttt ccagaaagct acccaggcat acagccaatc
     1801 gatcaccctg gccgccaaca tcaaatccct gtccgaagca tcgcatctca acgtgttccc
     1861 cgattacaag aagctgctaa ccccgctgac tgccgaaatc cccgacctgc agaccttatt
     1921 cggcaacgga gactactgcg agtgcgatga ctgcaacgcc gtagatggcg cggccgccta
     1981 cctcgccgac atcctgcact acctggatga acgcccctcc aaacaagtcg gtatttccgt
     2041 gaaggattgg ctcctcctgc gcaggccgga tatctcggac atcgacctgg attgcgataa
     2101 taccaatacc gagatacctt atatcgatat agcgtgcgaa ctgatggaag actatatcaa
     2161 ttcgcccacc atccagatcg ccataccggc tcccgtcaaa ggcaagatcg accccggcac
     2221 cgtcacttcc atccagacca aattcacggc catcgggatg aagaatatca gcgattccct
     2281 caccactaat gccatcgtat cggaaagcta ttccagtaca tggtataacg gtaccacgaa
     2341 ggttaccacc accaactgga tcattcggga tacgctggtc gtgctgaagg tcaccgacgc
     2401 ctccgggggt aaattcaatg tcaagctgct ccatcagacc ctcctcagct ccgatgaggt
     2461 cagcgccaat ccggaataca tcaacctgcc cgtctaccag gacaatgccg ccctatccat
     2521 gaaaggcatc ctgaacaatg cgcagcgtcc cttctccctg ccctttgacc tcttcgagac
     2581 cgaaggagaa ttgtacctcc tcaaactggg tatcaataaa tatgacctga tcaagacctt
     2641 ccagaaggaa cacgatattt ccggaccgcc ttccagcaac gacctcgctt atgcttatgc
     2701 gtggctagac gtcaccccgg cggaacgcac cctgatcttc caggcggata ccgtcaacca
     2761 aacccattac tggggcagca tcgcatccgg gacaacggtc ggtatcgatg atttcgaaaa
     2821 tgcctccgga ctggcctaca gcgatatcct caacctgctt ggcctgacgt tggtcaaccc
     2881 cggccaggtc atcgtaatac aacatgacgc tcccccctcc tgtgacacta ccaagcagcg
     2941 gcttatcaat atgtcgcccg gcgccttcga cattatgcac cggttcctcc gcctttggcg
     3001 gaagacatcc ctggtcatgt ccgacctgga tgccatcgta cggtccactg cagtgggcgc
     3061 aggaaagatc gacccggcat ttgcagtagc cttccagcca ttcctgctcc tgcagcaggc
     3121 atggtcgctc agcaccccgc agttgctctc cttctaccag aatatcgaca cgaccggcag
     3181 cgacaacctc tatctgcaga ccttccagaa caaatcggtg accaatcccg taaaccccga
     3241 cttctccatc accaacgtca ccgccggagc catggcaatc tctcccttgc atcaggccgt
     3301 catcacagca tcgacaggca tatcgtcaac cgacctgacc accctcatcg ccctcactga
     3361 cggaaaactg tccctcgcca acctttccta tttctatcgg gtctacctcc tgtcacaagc
     3421 cctgtccata tccgttgcag acctgctcca tgcggtcgac cttttcaata tcaacccctt
     3481 ctccggtccc gcattgacgg cttcattcag ggacaaattc accttcctta ccggggcggg
     3541 cttcagcctg gacgacttga attacgtcct ccgccagcag gatacccccg ataaccaata
     3601 tatcgcaagc aacaagcaga tcgccgccaa tctcgcaacc ctgcagaccg ctttactgtc
     3661 ggcgacagcc gcgaccacgc ccgcaccaga tccaaaaggc gccctgcttg ccaagtggct
     3721 cgcagatccc ctcttagcct gggacccggc cctcgtgacc aagctgttcg atatcctcaa
     3781 tacatcggat gatggccaat acgcccagaa gatcaccaac gatacaggct tcctgaatga
     3841 cctcagggtg cagtacaatg caaaaaatgt ctccgccaac ctttccgcac ttccggcttt
     3901 ctccccggca gacctggaca tataccaatc ctattccacc cagatcagct atgacagcgt
     3961 cggacttcag cttatctggc gagggtgcat gagcacagct gatcagaccg ccctgaatgg
     4021 cctggccagc gccaccacgg attttaagaa cgctgtaacg aaccttttca accagaccca
     4081 gctgaccagc agcgcctcag ccaatgtatt catacactcg gctgccgata ttacgaccct
     4141 gtcggccatc ccgttcacat ccgtggcgca gcgattcgcc ttcttcctga atgccatctc
     4201 gccggtctat ctgaccctca tgcagcaaca ggctattgcc tcccagatca ccacatggtt
     4261 cagcgtggca agacagatca ctgcgcaact gatgacatcg atacccggga tctttacaga
     4321 ctatactgcg acaccgttta ttaccaggca gcttgacctc accgcagtca attaccccaa
     4381 ccaattcaac cggtacctgc aactgcagaa gatctgtttc ctggcggcca ggctacagtt
     4441 gtccgtcacc gatatacaat ggcagcttgc caatgccgct gccgccggca gcctgaatta
     4501 tttatcgctg ccgcttgcat cgctgacggg accggtgacc accttcccgc aattcataaa
     4561 ccttatcgat atactgaagt tctcacagca tcatcccgtc gtcattacgg accccaccgt
     4621 tacgcctccg attaccctgt ccgtgtacga catcctcacc gatgccatta acgccaagcc
     4681 catcgcccag atcgaaaagg acctggtgac cctcaccggc tggaatgcgg ataatctcaa
     4741 aaaactggtc gaagcgccta attaccttaa cctcacttca ccctcggatt ttacctctcc
     4801 cgcgatcctg tcccggctgg acgcttgctt ctcagtcctg tccctgttga atatcggcgc
     4861 cgatgacgcg attgcctgga gcaaagactc attgtcatat gacgatgccg tcaagatcgt
     4921 acagaccttg aagacaggct actccgatgc cgattgggtc cccgtcacac aacccctgca
     4981 ggataaactg cgggagaaga agcgggatgc ccttatcgcc tggctgctgg ctaaccccgg
     5041 atcacagagc tgggtcaccg atacggacct ttatagctat ttcctcctgg acgtggagat
     5101 gtgcgcatgc caacccactt ccaggatcgt acaggccacc aattcagtgc agctttttgt
     5161 ccaacgctgc ttcctcgcac tggaattgaa tatagccgtc gatactacgg tcgattccaa
     5221 ttggacccaa tggcagtgga tgaaattctt ccgcctgtgg caggcgaatt atgaagtatt
     5281 cctctggccg gagaactgga ctgagcccga gctcctgcct gtccaatcat cttatttcac
     5341 cgacctgcag accgccctct cccagaatga ggtcacggag tctaacgtag aggatgcttt
     5401 tatggcctac ctccaaagcc tggacggtgt tgcgagactc gaggtcaaag gcatgtggta
     5461 cgacgacccc aataagcagt tctatgttgt tgcccgcact tacggcggtg atccgaaaat
     5521 atattacttc cggcagctgt ccatcgtcga cgggatctgg accccatggc agaagatcga
     5581 cctggatatc aagagcgacc atattatacc cgttgtctac aacaaccgtt tctatatctt
     5641 ctgggccgta ttcggcgaga aatccgatac cccgacatcc ctgaatgtcc ccgatatcaa
     5701 ggaaagcagc ttccccgttc cccagcctac caagtactgg cagatacaga tggcatacag
     5761 cgaatacagg aacggaaaat ggctcgcgaa gaaaatatct gccgacgacg atactggtat
     5821 catccaggcc caggagaacg atgggacgac tcctatatac ccggacaagt cggccttcct
     5881 gttcaccgcc ctggacatgc cgcagatcga tctgaaaacg cttctggagc aatacaagaa
     5941 cctgcccgct accggtggca agaaggagtc cttcgcacaa ttcctgctgg ataccgtagc
     6001 gcaagccctt tcccagaacg gcgatctcat cattaactgc tattacacca agcaggagtc
     6061 ttcagggcct tattacgaat acaagggctc attcgacctg gacccctgtc gcggttaccc
     6121 ggtagtagta accaaggcag tccagatcca cccctggatc ttcgataaca gtgccctctc
     6181 cagcatgctc gacgacgagg actccacgaa tgacctggca ctctccagtg tacctat
//
LOCUS       DATGFG010001162         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_937429, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001162 DATGFG010000000
VERSION     DATGFG010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937429"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VKQ52_22025"
     CDS             complement(<1..572)
                     /locus_tag="VKQ52_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89953.1"
                     /translation="MGNDTTAVGGGVTIGGQGVRERTFDAIVIGSGISGGWAAKELCE
                     QGLKTLVLERGRNVVHNRDYPTATMAPWEFPHRKEMPRKFVEENPLITTAAGFGEDTA
                     HFFIRDKDHPYVQEKPFDWIRGYQVGGKSLIWGRACPRWSHYEFTNPARFGYGIEWPI
                     SYEEIAPWYSHVERFSGICGNRDGLEAMPDG"
     gene            complement(562..1257)
                     /locus_tag="VKQ52_22030"
     CDS             complement(562..1257)
                     /locus_tag="VKQ52_22030"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HLZ89954.1"
                     /translation="MKYTTLYGIGVALLMAAAPRFSASPGAKVGEPDPAQHSKQLFNG
                     KDLSGWSHVGPGSMVVEGGLLHGVGGMGLLYYAGEKFADCTFHIVYRMKDENDNSGVF
                     IRIPIEPREEWMPVHYGYEVQIDNHPERSKEDDYHITGTLYSLTKPLAKPGKPGPQWN
                     TMDITLEGPRTVVSVNGVKVTDYREGEPVPARKFDFEPMRGRRPDNGYFGIQNHSNND
                     IVYFKEISVRYGK"
     gene            1359..1817
                     /locus_tag="VKQ52_22035"
     CDS             1359..1817
                     /locus_tag="VKQ52_22035"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLZ89955.1"
                     /translation="MATTKKTNGDAAIAGFVDAIICVRQAIKQLAQQKLRELHDREIT
                     YEMLQVLSVLWNKHQINQQEVANATQKNKASLTPLIDSLVRMGLITRSEDPDDRRNKI
                     ITLTPKGKEYRKKFSPMIEDIYEVVRGDISDAKLREITTLLLIVKENILL"
     gene            1814..2269
                     /locus_tag="VKQ52_22040"
     CDS             1814..2269
                     /locus_tag="VKQ52_22040"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HLZ89956.1"
                     /translation="MNIVKSINAWGDRHHPKLLDIIRMLLGLFLLSKGVIFLNNAGYL
                     RYLIIEKGAINQSPGMISAILYYVTYMHVLGGGLIFLGLYTRLWALLQIPIVFAAVFF
                     VNITAPYVNSELWLSILVLALLCLFLVIGSGPWSLDRLLSTSKTTGEEL"
     gene            2266..>2640
                     /locus_tag="VKQ52_22045"
     CDS             2266..>2640
                     /locus_tag="VKQ52_22045"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLZ89957.1"
                     /translation="MKKILVLVDFSAPSVNAVAYAADLAGDTGFGEIVLMANCYVPLF
                     EQIVPSPDLVQVGAADIARKQEKTLLQLMALKSEIEKKLPATVLVRVILGSQSLLRSV
                     LEQVAKEEPSVVIIGSNSRAPME"
BASE COUNT          585 a          833 c          667 g          555 t
ORIGIN      
        1 ccgtccggca tcgcctccag cccgtcgcgg ttgccgcaaa tgccggagaa gcgctcgaca
       61 tgcgaatacc agggcgcaat ctcctcatag ctgatgggcc actcgatgcc atacccaaaa
      121 cgcgcggggt tggtgaattc gtagtggctc caccgcggac aggccctgcc ccagatcagc
      181 gacttgccgc ccacctggta accccggatc cagtcgaagg gtttctcctg tacataggga
      241 tggtccttat cgcggataaa gaaatgcgcc gtgtcctccc caaaacccgc cgccgtggtg
      301 atcagcgggt tctcctcgac aaacttccgc ggcatctcct tgcggtgcgg gaactcccag
      361 ggcgccatcg tcgcggtggg gtagtcgcgg ttatggacca cattccggcc ccgctccagc
      421 accagcgtct tcagcccctg ctcgcacaac tccttcgcgg cccagccgcc gctgatcccg
      481 ctcccgatca cgatggcatc aaacgtccgc tcccgcaccc cctgcccgcc aatagtcact
      541 cccccgccaa cggctgtagt atcatttccc ataacgtacc gagatttcct tgaaataaac
      601 aatatcgtta ttgctgtgat tctggatgcc gaaatacccg ttgtccggcc ggcggccacg
      661 cattggttcg aaatcgaact tcctggcggg caccggctcc ccctcgcgat aatccgtcac
      721 cttcacccca ttcaccgaca cgacggtccg cggcccttcg agcgtgatgt ccatcgtatt
      781 ccactgcggc ccgggcttcc ccggcttggc cagcggcttt gtcagggaat aaagcgtccc
      841 ggtgatatgg tagtcatcct ccttcgaccg ctcgggatga ttgtcgatct gtacctcgta
      901 gccataatga acgggcatcc attcctcccg cggctcgatg ggaatgcgga taaagacacc
      961 cgagttgtca ttctcatcct tcatccggta gacgatatga aaggtgcagt ccgcgaactt
     1021 ctcgccggcg tagtaaagca gccccatccc gccgacaccg tgcaacagac cgccttcgac
     1081 gaccatactc cccggcccca catggctcca gccgctcaga tccttcccat tgaataattg
     1141 tttggaatgt tgcgccgggt ccggttcccc aaccttcgcc ccgggtgacg ccgagaatct
     1201 cggcgccgcc gccatcagca gggcgacacc gatcccatac agtgtagtat acttcataaa
     1261 gcaagcttag tccttctaag ataagcaatt ccacggttaa aaaaatagtt aaacgtttaa
     1321 ctattaaatc cctaacttta tatcggcaac accataccat ggcaacgaca aaaaagacca
     1381 acggggacgc cgccatcgcc ggtttcgtgg acgccatcat ctgcgtccgt caggcgataa
     1441 agcaactggc ccagcaaaag ctccgcgaac tgcacgaccg ggagatcacc tacgagatgc
     1501 tccaggtgct gagcgtgctc tggaacaaac accagatcaa tcagcaggaa gtagccaacg
     1561 ccacccaaaa gaacaaagcc agcctcaccc cgctgatcga cagcctcgtc cggatgggcc
     1621 tcatcacccg ctcggaagac cccgatgaca ggaggaacaa gatcattacc cttaccccga
     1681 aagggaagga gtatagaaag aaattctccc cgatgatcga ggacatctat gaagtggtca
     1741 ggggagatat ctccgacgcg aagctgcggg agatcaccac cctgttgctg atcgtaaagg
     1801 aaaatatatt gttatgaata tcgtcaaaag tatcaatgcc tggggcgaca ggcaccaccc
     1861 caaactcctg gacatcatac gaatgctgct tggtctcttc ctgctgtcca agggggtcat
     1921 cttcctgaac aacgccggct atctgcgcta cctgatcatt gaaaagggcg ccataaacca
     1981 gtcgccgggc atgatcagcg ccatcctcta ctatgtcacc tatatgcatg tattgggcgg
     2041 cggactgatc tttctcggat tgtatacgcg gttgtgggcc ctgctgcaga tcccgatcgt
     2101 ctttgcggcc gttttcttcg tcaatatcac ggccccctac gtcaattccg aactctggct
     2161 gtccatcctg gtactggcct tattatgcct tttcctcgtg atcggttccg ggccctggtc
     2221 gctcgaccgg cttttatcaa cctctaaaac aacaggggag gaattatgaa aaagatattg
     2281 gttctcgtcg atttttcggc gccctcggtc aacgcggtcg cctatgccgc cgatcttgcc
     2341 ggcgacaccg gtttcgggga gatcgttctg atggcgaatt gctatgttcc gttattcgaa
     2401 cagatcgtcc cctcacccga cctggtccag gtgggcgccg cggatatcgc ccggaagcag
     2461 gaaaagaccc tgctgcaatt gatggcgctg aagtcggaga tcgaaaagaa attgccggcg
     2521 acggtactgg tcagggtcat cctcggcagc cagtccctcc tgcgctcggt gctggaacag
     2581 gtggcaaagg aagaaccttc cgtcgtgatc atcggcagca acagccgtgc gcccatggaa
//
LOCUS       DATGFG010001163         4613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1443575, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001163 DATGFG010000000
VERSION     DATGFG010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443575"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VKQ52_22050"
     CDS             complement(<1..833)
                     /locus_tag="VKQ52_22050"
                     /inference="COORDINATES: protein motif:HMM:NF022218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HLZ89958.1"
                     /translation="MSLPKVSIDLSTGNLGLVGTNVDGIAALILIAATSGLTNINTPK
                     QLTKLADAGTQFGITAAANPFLYTQIQEFYNEAGDGSPLWTIIVPDTNTMTGVVDPTN
                     ATGAARLLLDTAAGAVKLLGIAKLPPAGYDQTTNDLDPDVYTAIPKMQALFNYYFDGN
                     TRFVPFRSLIEGRGYVTLDGVQDITTKASPAVGVVLGGSISGLKMASVGLVLGRASKI
                     PIHHNLGRVKDNKISVDTIYWNALQYSQVESLIGNLHDLGYITFRQFPGKNGYFITDD
                     NT"
     gene            complement(868..1098)
                     /locus_tag="VKQ52_22055"
     CDS             complement(868..1098)
                     /locus_tag="VKQ52_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89959.1"
                     /translation="MDKNKAIEHAKPYFNTWPDADAFHITSDGQAFFTDQDAQAHAAG
                     TRKDKIVHKVQRHETEDTAPGGDTEEPKGKTK"
     gene            complement(1101..1499)
                     /locus_tag="VKQ52_22060"
     CDS             complement(1101..1499)
                     /locus_tag="VKQ52_22060"
                     /inference="COORDINATES: protein motif:HMM:NF040141.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HLZ89960.1"
                     /translation="MELLIKRTYHEDGTNGELFIDGVQKCFTIELPWHQNDHGISCIP
                     EGRYHLAKRNTIKFGNHLLVENVPGRSFILIHPANDALKELLGCIAPVSVLTGPGRGD
                     KSRAQLDDIVQTTYAAMSRGEDVWLTLQKA"
     gene            complement(1499..2152)
                     /locus_tag="VKQ52_22065"
     CDS             complement(1499..2152)
                     /locus_tag="VKQ52_22065"
                     /inference="COORDINATES: protein motif:HMM:NF042453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6549 family protein"
                     /protein_id="HLZ89961.1"
                     /translation="MLGICCFLLLDRCGKQRTIAQLQNEKAFLTDTVVTYIDAGGLEH
                     AAIPERTLTPSQVKALLPDQVKSLTGKLNAKISDFNNLVTATTQTSQVIETPTIAHDT
                     VYAAGDFAPGFHYHDQWIDIEGTVLKDTVDLKYTIRDSLWFSTYWKRTGWFNKELLLD
                     GFSADPHTTVTGIKGISLDKERRPVRLSLGPVFSYSLINGAFKPTVGVGLQYNLIRF"
     gene            complement(2223..2480)
                     /locus_tag="VKQ52_22070"
     CDS             complement(2223..2480)
                     /locus_tag="VKQ52_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89962.1"
                     /translation="MKKFLETMNNFLSGIGKTQITNIIAVISVLGVFGLLFLLCFRSI
                     PKENSQLMYMAVSQVLGLGFGIVIGFYFGSSKTEADKRNNV"
     gene            complement(2477..2782)
                     /locus_tag="VKQ52_22075"
     CDS             complement(2477..2782)
                     /locus_tag="VKQ52_22075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89963.1"
                     /translation="MTNSTETKVKAMLTNALLTIIAGLITLFGTITFNVFKGISEKLD
                     TLATRQVRTEKDIETLKGNVSDQREETRRLTGDVNFLHTVQEAEADLLEKMSKLKKP"
     gene            complement(2793..3878)
                     /locus_tag="VKQ52_22080"
     CDS             complement(2793..3878)
                     /locus_tag="VKQ52_22080"
                     /inference="COORDINATES: protein motif:HMM:NF040375.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HLZ89964.1"
                     /translation="MRQWTKALGVIFSIGLLSSPAFTAMQTLSETTGLPFFILLAVTF
                     LLYRVIPKIRGVRTAGIQVEIWERDIIGNLFKNNDFLLQAYNADQYVLQGKVVHIPQA
                     GGLQNVVKNRNALPAAVTQRTDVDITYALDEYTSDPFLISNAETVELSYDKRMSLMTE
                     TIQALRQTVADTILINWAPSNGFQLLRTSGGAAGKTAAAYTPGATGVRKTLSVLDVIA
                     AQAAMDGQDIPDDDRYALLDAQMYSQLLAELSQTQYRDFASGQDLARGIIGEMYGFKF
                     YKRSSVLIYDGNPLPKAYGAAGAATDNAASLFWQKNSLERALGEVKMFERMNDPQYYG
                     DVYSVLLRMGGRIRRNDGKGLIAMAQQ"
     gene            complement(3919..>4613)
                     /locus_tag="VKQ52_22085"
     CDS             complement(3919..>4613)
                     /locus_tag="VKQ52_22085"
                     /inference="COORDINATES: protein motif:HMM:NF021627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HLZ89965.1"
                     /translation="PALMLAGQTGPTVTQWEVAEASIVDIPGCKNALAIREDGKLIKQ
                     TRANAAEFKLKLSKIFDPDMDFLKLAAKIVGLPETATQIEVENKIAELQNGLTAEAGR
                     TQDLQQKLTASQTEITNLKREQTDRKIADLVDGAVTARKITAKEAEHYRKLAAVDFVS
                     TQAILDNLKPYESVEKRLKEGKEGDELAELSKLSWDQLDQQNKLALVKEKYPDLYRTK
                     YKATFNKEPKNQ"
BASE COUNT          989 a         1183 c         1223 g         1218 t
ORIGIN      
        1 gtattgtcgt ccgtgatgaa ataaccgttt ttgccgggaa actgccggaa ggtgatatac
       61 cccaggtcgt ggaggttgcc gatcaatgat tcgacctgac tgtattggag agcgttccag
      121 tagatagtat ctacgctgat cttgttgtct tttacgcgtc ccaaattgtg atgaatgggt
      181 attttactgg ccctacccaa caccaaccct acagaagcca ttttcaaccc gctgatcgag
      241 ccgcccagga caacaccaac cgccggggac gctttggtgg taatatcctg caccccgtct
      301 agggtaacgt aaccccgtcc ttcgatgagc gaacgaaaag gaacaaatcg ggtattaccg
      361 tcaaaataat agttaaacag tgcttgcatc ttgggaatgg ccgtatatac atccgggtcg
      421 aggtcgttgg tcgtttgatc gtacccagcg ggcggcaatt tggcgatccc caggagtttg
      481 acggctccgg cagccgtatc gagcagcaag cgggcggccc cggtggcatt cgttgggtcc
      541 accaccccgg tcatggtatt ggtatccggt acgatgatcg tccaaagcgg gctgccgtcc
      601 cctgcctcat tatagaattc ctgtatctgc gtataaagga aagggttggc tgctgccgtg
      661 atgccgaact gtgtaccggc atcggcgagc ttggtgagct gcttgggggt gttaatgttc
      721 gtcagccccg aggtagcagc aatcaggatc agggcggcga tgccgtccac gttggtcccg
      781 acgaggccga gattgccggt cgaaaggtct atggaaactt tgggtaagga catgagtacg
      841 aattaaatca ttacaggaag ggatcactta tttggtcttt cccttcggtt cttccgtgtc
      901 cccgccggga gcagtgtctt ccgtttcatg gcgctggacc ttgtgaacga ttttatcttt
      961 ccgggtgcct gccgcatgcg cctgggcatc ctggtccgtg aagaatgcct gcccatcgga
     1021 cgtgatatga aaggcatctg cgtccggcca ggtgttgaaa taaggtttcg cgtgttcgat
     1081 agctttgttc ttatccatca ttatgctttt tgaagggtta accatacgtc ttcgccccgg
     1141 ctcatggcgg cataggtggt ctggacaata tcgtccagtt gggcgcggct cttatctccc
     1201 cggccgggtc cggtcagtac actgacggga gcgatacacc cgagcagctc tttgagcgcg
     1261 tcattagccg gatgaataag gataaaggag cggcccggta cgttctccac caggaggtgg
     1321 ttgccgaatt tgatcgtatt gcgtttggca agatgataac gaccttccgg gatacaactg
     1381 attccgtggt cgttctggtg ccacggcagt tcgatggtga agcacttctg cactccgtcg
     1441 ataaatagtt ccccgttggt gccatcttcg tggtaggtac gtttgatgag tagttccatt
     1501 agaaacgaat taaattgtat tgtaatccca cgcccacggt gggtttgaac gctccgttaa
     1561 tcaggctata actaaacaca gggccgagcg acagccttac ggggcgccgc tctttatcca
     1621 ggctgattcc cttaatccct gtgacggtgg tatgcggatc ggcggagaag ccgtccaata
     1681 aaagttcttt gttaaaccat ccggtacgct tccaataggt ggaaaaccaa agactgtccc
     1741 ggatggtata ttttagatcg acggtgtctt tcaatactgt cccctctatg tctatccatt
     1801 gatcatggta atgaaagccg ggagcaaaat cacccgctgc atataccgta tcatgggcaa
     1861 tggtgggggt ttcaatgacc tggctggttt gggtggtggc cgtgaccagg ttgttgaagt
     1921 cggagatctt ggcattcaac ttaccggtca ggcttttaac ctggtccggg agcagggcct
     1981 tcacctggga aggggtgagc gttctttccg gtatggcagc atgctccagt cccccggcat
     2041 cgatataggt tacgaccgta tctgtcagaa acgccttttc attctgtagc tgtgcaatgg
     2101 ttcgttgttt cccgcagcga tccaggagaa gaaagcagca gatacctagc aaagcaataa
     2161 taatacattg cggaatgctg atacggagct ttgaaaacat agggtaacgg attaaagacc
     2221 ttttaaacat tgtttctctt gtcggcttcc gtcttgctag agccgaaata aaagccgatg
     2281 acgataccaa agcccaggcc caacacctgg ctcacggcca tatacatgag ctggctgttc
     2341 tctttgggga tggaccgaaa gcaaagcagg aataacaatc caaatactcc caggacgctt
     2401 atcacggcga taatattggt gatttgtgtc ttgccgatgc cgctaagaaa attgttcatg
     2461 gtttcgagaa attttttcat ggtttcttga gtttgctcat tttctccagg aggtcagctt
     2521 cggcttcctg gaccgtatgt aagaagttga catcgccggt taaccggcgg gtttcctccc
     2581 gctgatcgct gacgttaccc ttcagggttt ctatatcctt ttcggtccgt acttgcctgg
     2641 tggccagggt atcgagcttt tcactaatgc ctttgaaaac attaaaagtg atcgttccga
     2701 aaagggtaat taacccggct atgatggtaa gcaaggcatt ggtcagcatc gcctttactt
     2761 tcgtttccgt actgttcgtc atggtaatgg ttttattgtt gcgccattgc gatcaatcct
     2821 ttgccatcat tacggcggat acggccaccc atccgcagga gcacactata cacgtccccg
     2881 taatactgcg gatcattcat ccgctcaaac atcttgacct cgccgagcgc ccgctccagg
     2941 gaatttttct gccagaacaa ggaggctgca ttgtcggtag ccgcacccgc cgcgccgtag
     3001 gctttgggca gaggattgcc gtcatagatc agcacggagc tacgcttgta aaacttgaag
     3061 ccgtacattt ctccgataat cccccttgcc aagtcctggc ccgaggcgaa gtcacggtat
     3121 tgggtttggc ttagttcggc caggagctgg ctgtacatct gtgcgtcgag cagagcatac
     3181 cggtcgtcat cgggaatgtc ctgtccgtcc attgcggcct gggctgcaat cacatccagg
     3241 acgctcaggg tcttgcggac accggtggct ccgggcgtat aagcggcagc cgttttcccg
     3301 gcagcccccc ccgaagtgcg aagaagctgg aagccgttgg acggagccca attgatcagg
     3361 atggtatcgg ccaccgtctg ccggagcgcc tggatcgttt cggtcattaa gctcatgcgt
     3421 ttgtcataac tcaactccac cgtttccgca ttggagatca aaaagggatc ggaggtatat
     3481 tcgtccaggg cataggtaat gtccacgtct gtacgctgtg tcacggcggc gggcagtgca
     3541 ttccggtttt tgacaacgtt ctgtaatccc ccggcctgtg gtatatgtac caccttgccc
     3601 tgcaaaacat attggtcggc gttgtacgcc tgcaagagaa aatcgttgtt cttaaacagg
     3661 ttgccgataa tatcgcgctc ccatatttcc acctggatac ccgccgtgcg cactccccga
     3721 attttgggga tcacccgata aagcaggaag gtgaccgcca aaaggataaa gaaaggaagg
     3781 ccggtcgttt cggatagggt ctgcatcgct gtaaaggcag gcgaggaaag caaaccgata
     3841 ctaaagatca ccccaagggc tttcgtccat tgtctcattc aaagggtatt taaaaagttt
     3901 ttaaagggta cggactaact attggttctt cggctctttg ttgaaagtgg ctttgtactt
     3961 ggtcctgtac aaatcaggat acttttcttt gaccagcgcc agtttgttct gctgatccag
     4021 ttggtcccag gatagcttgc tcagttcggc cagttcgtcg ccctccttgc cttctttcaa
     4081 ccgcttctcg acactttcgt atggcttcag gttatccagg atggcctggg tgctgacaaa
     4141 atccaccgcc gccagctttc ggtaatgctc cgcctccttt gccgtgatct tccgggcggt
     4201 aaccgccccg tccaccaggt cggcaatctt tcgatctgtc tgctcccttt tcagattggt
     4261 aatttccgtt tgggaggcag tgagcttttg ctgcaagtcc tgggtccggc ccgcttcagc
     4321 cgtgagaccg ttttgcagtt ccgcgatctt attctccact tctatttggg tggcggtttc
     4381 cggtaggccg actattttgg ccgcgagttt tagaaaatcc atatcgggat caaaaatttt
     4441 tgaaagttta agtttgaatt ccgcagcatt ggccctggtc tgtttaatga gcttcccatc
     4501 ttcccggatc gccagggcat ttttacaacc cggaatatct acgatggatg cctccgccac
     4561 ctcccactgc gttaccgtcg gtcctgtctg acccgccagc atgagagccg ggt
//
LOCUS       DATGFG010001164         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_872829, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001164 DATGFG010000000
VERSION     DATGFG010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872829"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VKQ52_22090"
     CDS             complement(<1..627)
                     /locus_tag="VKQ52_22090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ89966.1"
                     /translation="MKNKMRKSLLLVASVVGLCNIAFAQTVDQGKKFLYYQRYKSAND
                     VFDKILAGNPNNIDAIYWKGQTLLAQHDSTAAGDLYSKALQTNGNAPLLLAGMGGVEL
                     RMGKTADAKQRFETAISLTKGKDVNVLNAVADNNIDARNGDAQYAIEKLTQATQVKNF
                     NNSDTYVLMGDAYRKLIDGGNAVQSYQKALAMDPKDAEAKYKIGKIYET"
     gene            complement(745..1665)
                     /locus_tag="VKQ52_22095"
     CDS             complement(745..1665)
                     /locus_tag="VKQ52_22095"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLZ89967.1"
                     /translation="MGIGIGKGILTGIVICALLGGCTGKGARTAQETPTNGTIHISVD
                     ESFRPVIDSQIKVFESSFPNAHIVADYRPEAQCLRDLSTDSTRLILVTRGLTPEEGNF
                     YNDSFHLRPTFGVLAFDAIAVIVNKTSKDSIFEVKDLQDMLNGADTKHLPVMDGVSAT
                     STVRYAMDSILGGKPLGKNVTAARSSEEVINYVANNPRAVGFIGASWIADRNDPGDTS
                     FNKNVVVAAVRCKTCLGTTYVRPFQANIALNRYPLVRSLYYILKENFSGVGNNFVNFL
                     QYERGQLIFSKAYLWPAGMHFELRDVQLSK"
     gene            2128..2655
                     /locus_tag="VKQ52_22100"
     CDS             2128..2655
                     /locus_tag="VKQ52_22100"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HLZ89968.1"
                     /translation="MEKAPTTSGATATRPPASFLMVCLGNICRSPLAEGILQEKARAA
                     GLDWKIDSAGTNGYHVGEAPHHLSQKVARHNGIDIGSQRSRRFTAADFQRFDKIYAMA
                     GDVIDDMRRIAGRNFDPSKVELLMNEVHPGRDEDVPDPWYGPEPGYHEVYTMIDAAAN
                     RIIEKYGQPVPNRAS"
BASE COUNT          664 a          919 c          626 g          645 t
ORIGIN      
        1 cgtctcatag atcttcccga tcttatactt cgcctccgca tccttggggt ccatcgccag
       61 cgccttctga taagactgca ccgcattccc cccatcgatc agcttccggt aggcatcccc
      121 catcagcaca tacgtgtccg aattgttgaa gttcttcacc tgcgtagcct gggtcagctt
      181 ctcgatggca tattgcgcat ccccgttccg ggcatcgata ttgttgtcgg cgacggcatt
      241 caggacattg acgtccttgc ccttggtcag gctgatcgcc gtttcgaacc gctgcttggc
      301 atcggccgtc ttgcccatcc gcaactcgac accacccatc ccggccagca ataacggagc
      361 attcccattg gtctgcaacg ccttggaata caggtcaccc gcggcggtgg aatcatgctg
      421 cgccaacagg gtctgcccct tccagtagat cgcgtctata ttgtttggat tacccgccaa
      481 tattttgtca aacacatcgt tcgcgctctt ataacgctga taatagagga atttcttccc
      541 ctgatccacc gtctgagcga atgctatgtt gcagagacct acaacgcttg ccaccagtag
      601 taagcttttt cgcatcttgt tcttcattac ttaatcgttg tttagaagtg tgtaaattta
      661 ttgaaatctg gtttaaactt ttgtgaaaac agtattcctt tttttccgga gtgacggtcc
      721 tccggattct tagtgacaat ccgcctactt actaagctgc acgtcccgga gctcgaaatg
      781 catcccagcc ggccacaggt aggccttgct aaagatcaac tgtccgcgtt catactgcag
      841 aaaattgacg aaattgttcc ccaccccact gaaattctcc tttaatatat aatacaaact
      901 tcgaaccagt ggataccggt tcaaggcaat atttgcctga aaaggtctga cataggttgt
      961 tccgaggcag gtcttgcagc ggactgccgc cactaccacg ttcttgttga aactcgtatc
     1021 ccccggatcg ttccggtccg cgatccagct ggcgccgata aaacccaccg cccggggatt
     1081 attcgccaca taattgatca cctcctcgct gctccgggcc gcggtgacat tcttcccaag
     1141 cggcttcccc ccaaggatcg aatccatggc ataccgcacc gtactggtgg cagacacccc
     1201 atccatcact ggtaaatgct tcgtgtccgc cccgtttagc atatcctgaa ggtccttcac
     1261 ctcgaaaatg gagtccttcg acgtcttatt gacgatcacc gcaatggcat cgaacgccag
     1321 taccccgaag gtcggccgca ggtgaaatga atcattgtag aaattaccct cctccggggt
     1381 caacccccgg gtcaccagga tcagtcgcgt actgtccgtg gacagatcgc ggagacactg
     1441 cgcctccggc cggtagtcgg caacgatatg ggcattcgga aaagaagatt cgaaaacttt
     1501 gatctgggaa tcgatcacgg gccggaagga ttcatcgacg ctgatatgga tcgtgccgtt
     1561 cgttggcgtc tcctgggccg tcctggcccc cttgccggtg cagccgccca gcagcgcgca
     1621 aataacgatg cctgtcagaa tgcctttacc aataccaata cccatgatta attaaagtag
     1681 tttttttggc gcccgcgata cacacggaac accccataca gcaggcaaag acccgaaaag
     1741 atatatcgga aggtatcgtc gatccctata ggaacgccca gtttcggcgc caccaggaaa
     1801 aagatcccaa aacaaaaaat gatcaccccc attccataat ccaatatcga tctccttagc
     1861 tcgtacgact tcttacggct ctcctccata tacggcttcg atgccccgtc cttttcatca
     1921 gtttctggac cccgctttgg cacttccgaa tgctcgtttg ccatttttaa ttaatttttg
     1981 tggttacgcc cgcgcgctaa cccttggccg ggcaaattaa acaaataacc gtaaaaacaa
     2041 ccatctacac ataaataaga tgcccctgca acccctattc cataaatccc ctacccccct
     2101 tccccaaacc tccttacctt tgcggccatg gaaaaagcgc ccactacttc cggggccacc
     2161 gccacccggc cacctgcctc ctttctcatg gtctgtctcg gcaacatctg ccgcagcccc
     2221 ctggcggaag gcatcctcca ggaaaaagcc cgggccgccg gcctggactg gaagatcgac
     2281 agcgcgggaa caaatggcta ccacgtcggg gaagccccgc accacttgtc ccagaaagta
     2341 gcccggcaca acggcatcga catcggcagc caacgctccc gccgtttcac cgccgctgat
     2401 tttcaacgtt ttgataaaat atacgccatg gccggcgacg tcatcgacga tatgcgccgc
     2461 atcgccggcc gcaacttcga cccgtccaaa gtcgaactcc tcatgaacga ggtccacccc
     2521 ggccgcgacg aagatgtacc ggacccctgg tacggccccg aacccggcta ccacgaagtg
     2581 tatactatga tcgatgccgc cgccaaccgt atcatcgaaa aatacggcca gcccgttccc
     2641 aaccgcgcct cctgatccgc ccaaccaacc caaacaattc gcatgagcaa gaacaaaccc
     2701 agtctcccgc aaggcacccg cgactttgac ccggtcaccg tccgccagcg acgctatatc
     2761 ttcgatacga tccgtaccgt cttcgagctc tatggctacc agcccctcga aacccccgcc
     2821 atggaaaacc tcgacacgct catggggaaa tacg
//
LOCUS       DATGFG010001165         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1140717, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001165 DATGFG010000000
VERSION     DATGFG010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140717"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VKQ52_22105"
     CDS             <1..634
                     /locus_tag="VKQ52_22105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLZ89969.1"
                     /translation="VNPDTVISTEAVASLDSVVSIALRSRPEIRNGQLNIDISRYDLL
                     KARAGYLPILTASGTLSSSYYSRTADAYFKQLDNNFFQQVGVSLSVPIFTRRSVKTAV
                     ENAKIEVEQSKLSLLNTRTVLAQNIEQAYINVINAQGQYDAAVEGLRASAESFRIAGE
                     QLKVGASNLVDYLQQKTLYTQAFQVYVQAKYNAALSIRIYDFYMGIAIKL"
     gene            656..2002
                     /locus_tag="VKQ52_22110"
     CDS             656..2002
                     /locus_tag="VKQ52_22110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLZ89970.1"
                     /translation="MKRALIIIGILLLAGGAAWYFFFKKEEKPLVLQTERPRFGYISK
                     SVTATGTVQPVDTVTVGSQVSGTIKALYTDFNAKVKKGQLLAELDKSLFLAQVDQYKA
                     NLSLARANLAYQKSTFDRQSQLYTVGAISKADYENSQSQYQTAQASVESVQAQLNGSQ
                     KNLDYASIYSPVDGVVMTRNVSVGQTVAASFNTPTLFIIAKDISKMQVQAAVSEADIG
                     DVRIGLRSTFTVDAYPDITFTGTVNQIRLEPIVSANVVTYSTIITAPNADLKLRPGMT
                     ANIFVFTKEVDSAMLITAKALKYKPDESLAKKYKIIPDTIDEHEVQVEARRFANNNPL
                     SPPRHRHDSTARQVDSAPQTGTPAFVWVLFGDSLVEKKITTGLNNDTRVQVLSGLDAN
                     DEIVNGAEVATSATPKSGTVRSPFMPARRGGNTPRSGSSGGAGAGGSRGGAGGGGR"
     gene            2022..2816
                     /locus_tag="VKQ52_22115"
     CDS             2022..2816
                     /locus_tag="VKQ52_22115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ89971.1"
                     /translation="MSNQKILELHDIKREFRMGSEIVHALNGVSFDVVSGEFLTIMGS
                     SGSGKTTLLNTLGCLDKPTGGTYLLDGVDIRKLNRNELAKLRNGKIGFVFQSYNLLPR
                     TSALENVELPLLYNPSLSAKERKERAIKCLEAVNLADRLDHTPSQLSGGQQQRVAIAR
                     ALVNEPVMILADEATGNLDSRSSYSIMALFQELNSQGKTIVFVTHEPDIAAFSSRTIM
                     LRDGKVIKDSPNEDKKSAKEVLDSLPTSEDYLSSAAAEAATQTKPS"
     gene            2813..>3426
                     /locus_tag="VKQ52_22120"
     CDS             2813..>3426
                     /locus_tag="VKQ52_22120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ89972.1"
                     /translation="MSFANLLRIALKALQRNKLRAFLTMLGIIIGVASVIAMVAIGQG
                     SKQSIQQQLSGMGSNMIIIRPNSNVTAGARLDATNVQTLTLDDVKALQKQAPDLSAVS
                     PMANARGQAIYGNNNWPTTMQGVQPNYLSIIRQWPVQDGTSFTDRDVQTQSKVCLLGQ
                     TVIGNLFPNGGSPVGKYIRFGNVPLLVIGTLAPKGESAFGQDQD"
BASE COUNT          841 a         1140 c          873 g          572 t
ORIGIN      
        1 cgtcaacccc gacaccgtca tttccacgga agccgtcgcc tccctcgaca gcgtcgtctc
       61 catcgccctg cgctcccggc cggagatcag gaacgggcag ctcaacatcg atatttcgcg
      121 ctatgacctt ctgaaagcaa gggcgggtta tctgcccatc ctgaccgcat cgggaaccct
      181 ctcctccagc tattacagcc gcacggcgga tgcctatttc aaacagctcg acaataattt
      241 cttccagcag gtgggtgtca gcctctcggt ccccatcttc acccgcagga gcgtgaagac
      301 agccgtcgag aacgcaaaga tcgaggtcga acagtcgaag ctgagcctgc tcaacacccg
      361 caccgtgctc gcccagaaca tcgaacaggc ctatatcaac gtgatcaacg cccagggcca
      421 gtatgacgcc gccgtcgagg gcctgcgggc aagtgcggaa agcttccgca tcgccgggga
      481 acaactcaaa gtgggcgctt ccaacctcgt ggactacctg cagcaaaaga ccctgtacac
      541 ccaggccttc caggtctatg tccaggccaa atacaacgcg gcgctcagca tcaggatcta
      601 tgatttctat atgggaatag cgatcaaact ctaaatagca gttaaactct acataatgaa
      661 gcgagcactc atcatcattg gtatactcct gctggccggc ggcgctgcct ggtacttctt
      721 cttcaaaaag gaggaaaaac ccctcgtcct ccaaaccgaa agacccaggt tcggctatat
      781 ctccaaaagc gtaacggcca ccggtaccgt acaacccgtg gacaccgtta ccgtgggctc
      841 acaggtctcc ggcacgatca aggccctcta caccgatttc aatgcaaaag tgaaaaaagg
      901 acagctgctc gccgaactcg ataaatccct cttccttgcc caggtggatc aatacaaggc
      961 caatctctcc ctcgcaagag cgaacctggc ctatcaaaaa agtactttcg accggcaatc
     1021 ccagctctat accgtcggcg ccatcagcaa ggccgattat gagaactccc aaagccagta
     1081 ccagacggcc caggccagcg tcgaaagcgt ccaggcccag ctcaacgggt cacagaagaa
     1141 cctcgactac gcaagtatct attcccccgt ggacggggtc gtcatgaccc gtaacgtcag
     1201 tgtcggccag acggtggcgg caagcttcaa taccccgacc ctctttatca tcgcaaagga
     1261 catcagcaag atgcaggtac aggcggccgt atccgaggcg gatatcggcg acgtccgcat
     1321 cgggttgcgg tccaccttca ccgttgacgc ctatcccgac atcaccttta ccggcaccgt
     1381 caaccagatc cggctcgagc ccatcgtctc cgccaatgtc gtcacctatt ccacgatcat
     1441 caccgcgcca aacgccgatc tgaaactcag acccggcatg acggcgaata tcttcgtctt
     1501 tacaaaagag gtcgacagcg cgatgctgat cactgccaag gcgttgaaat ataagccgga
     1561 cgaaagcctc gcaaaaaaat acaagatcat ccccgatacc atcgatgagc acgaggtaca
     1621 ggtggaggca aggcgcttcg ccaacaacaa cccgctaagc ccgccccggc accggcatga
     1681 ctccaccgca agacaggtgg attcggcgcc ccagacgggt accccggcct ttgtatgggt
     1741 actgttcggc gacagcctgg tcgagaagaa gatcaccacc gggctcaaca atgacacccg
     1801 ggtgcaggtg ctgagcggcc tggacgccaa cgacgagatc gtcaacggcg ccgaggtcgc
     1861 caccagcgcc acaccaaaat ccggcacggt gagaagcccc ttcatgcccg cgcgccgggg
     1921 cggtaacacc ccccgcagcg ggagcagtgg aggagccgga gccggcggca gtcgtggcgg
     1981 agcaggaggc ggcggcagat agccgtccaa aagcaaaaaa catgagcaat cagaaaatac
     2041 tggaattgca tgatatcaaa cgggagttcc ggatgggctc ggagatcgta catgccctga
     2101 acggggtgtc cttcgacgtc gtatccggag agttcctgac catcatgggc agcagcggct
     2161 cgggcaagac caccctgctc aacaccctcg gctgcctcga caaacccacc ggcggaacct
     2221 acctgctgga cggcgtcgac atccgcaaac tcaaccgtaa cgagctggca aaacttcgca
     2281 acgggaagat cggcttcgtt ttccagtcct acaacctgct cccccggaca tcggccctcg
     2341 agaacgtcga gctgcccctc ctctacaacc cctccctaag cgcaaaggaa aggaaagaac
     2401 gcgccataaa atgtctcgag gccgtcaatc ttgccgatcg cctggaccat acccccagcc
     2461 agctctcggg cggtcagcag caacgcgtcg ccatcgcccg ggccctcgtc aacgaaccgg
     2521 tgatgatcct cgccgacgaa gcgacaggca acctcgactc ccgtagttcg tatagtatta
     2581 tggccctgtt ccaggaactc aattcccagg gcaagaccat cgtcttcgtc acccatgaac
     2641 ccgatatcgc cgccttcagc agccggacca tcatgctgcg ggacggaaag gtgataaagg
     2701 acagccccaa tgaagacaaa aagtcggcaa aggaagtact ggactccctg cctacatccg
     2761 aagattattt gtcatcggcc gcggccgaag ccgcgacgca aactaagcca tcatgagctt
     2821 cgccaacctc ctccggatcg ccctgaaggc gctgcaacga aacaaactgc gggccttcct
     2881 caccatgctg ggcatcatca tcggcgtggc ctcggtcatc gccatggtgg ccattggtca
     2941 gggctccaaa caaagcatcc agcaacaact ctccggcatg ggctccaata tgatcatcat
     3001 ccgcccgaac agcaatgtca ccgccggcgc ccgcctcgac gccaccaacg tccagaccct
     3061 taccctggat gacgtaaagg cgctgcagaa acaggcgccc gacctcagcg ccgtctcccc
     3121 catggccaat gccaggggcc aggccatcta cggcaacaac aactggccga cgaccatgca
     3181 gggcgtccag cccaactacc tgagtatcat tcgccaatgg cctgtccagg acgggacgtc
     3241 gttcaccgac cgcgacgtcc agacccaaag caaggtctgc ctgctcggcc agaccgtgat
     3301 cggaaacctg ttccccaacg gcggcagccc ggttggaaaa tatatccgct tcgggaatgt
     3361 tcccctgctg gtcatcggca ccctcgcccc caagggagaa agcgccttcg gccaggacca
     3421 ggacga
//
LOCUS       DATGFG010001166         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1673540, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001166 DATGFG010000000
VERSION     DATGFG010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673540"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VKQ52_22125"
     CDS             complement(<1..207)
                     /locus_tag="VKQ52_22125"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HLZ89973.1"
                     /translation="MKLKLNIEDMTGEFFEDTRLLGIVAPVKNYQFCWQLNNRLRFDF
                     RIDNDIEIQLTKRQRKYFFGVYEYH"
     gene            complement(220..1878)
                     /locus_tag="VKQ52_22130"
     CDS             complement(220..1878)
                     /locus_tag="VKQ52_22130"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLZ89974.1"
                     /translation="MISRIKFLTPILVLPCLITPASAQKLKKTDKTTLSNLQAHIRYL
                     ADPHLEGRRTGTPGEKAASDYISSALAQAGIQPRGDNNGWLQTFEIDQGREVSRDAFF
                     VVNDHPFLLEKEFFPLALSAATAVSGSPAIALQESGVPWFFDLRELLEANAGNPHFDL
                     PGAIRAKAAACTKKGATALILYNSSRIADNLAFDPHDKPEPVTIPVVYITREAKRKYL
                     KDEAASLDIRLKVGFTEKKRTGHNVVGYLNNGAPTTVVIGAHYDHLGHGEDGNTMYHG
                     KDSVTYTGADDNASGVAGMIELARMLAASRLKGNNYLFVAFSGEEQGLFGSKYLVNHS
                     PVDVKKLNYMINLDMIGRLNDSSHALTLGGFGTSPAWGEICNTIRERKEFTLRLDSSG
                     TGPSDHTSFYLKDVPVLFFFTGAHPDYHMPGDNADKINYPGELQVIKFIYDIVETANT
                     RGRFVFTKTRETSPDLPRFSVTLGILPDYTFSGSGVRADGVSQGRPAEKAGLKAGDVI
                     VQLGSYPVTTMENYMEALGKFKKGDKTTVRYKRGGDTMESPVEF"
BASE COUNT          427 a          563 c          660 g          538 t
ORIGIN      
        1 atggtattcg tagaccccga agaagtattt ccgctggcgt ttggtcagtt ggatctcgat
       61 atcattgtcg atgcgaaagt cgaaccgcag ccggttgttc aactgccagc agaactgata
      121 gttcttcacc ggggccacga tgcccagcag ccgggtgtcc tcgaagaact cgcccgtcat
      181 atcctctatg ttcaacttta gcttcatgtt cgtcggctct tagaactcta cgggcgactc
      241 catcgtgtcg ccgccccgtt tatagcggac cgttgttttg tcgcctttct taaattttcc
      301 cagcgcttcc atatagttct ccatggtcgt gacgggataa ctgcccaatt gcacgatgac
      361 gtcgcctgcc ttcagccccg ccttttccgc cggccggccc tggctgacgc cgtcggccct
      421 gacgccgctg ccggagaagg tatagtccgg caggataccg agggtcacgc tgaagcgcgg
      481 cagatccgga gaggtctccc ttgttttggt gaagacgaac cggccgcggg tgttggcggt
      541 ttcgacgatg tcatagataa attttatcac ctgcagttca ccggggtagt tgatcttatc
      601 ggcattgtcg ccgggcatat ggtaatccgg gtgagcgccc gtgaagaaaa agagtacggg
      661 gacatccttg agataaaagg aggtgtggtc gctgggaccc gtgccgctgc tgtccagccg
      721 aagggtgaat tccttgcgct ccctgatggt gttgcagatc tccccccagg cgggcgaggt
      781 gccgaagccg cccagggtca gggcgtggga gctgtcgttc agccggccga tcatgtccag
      841 gttaatcata tagttcagct ttttcacgtc gacgggcgaa tggttcacca ggtatttgga
      901 gccgaacaag ccctgctctt cccctgaaaa ggcgacgaag agatagttgt tgccctttaa
      961 ccgggaggcg gccagcatgc gcgccagctc gatcatcccg gcgaccccgc tcgcgttgtc
     1021 gtcggcgccg gtgtaggtga cgctgtcttt tccatggtac atcgtattgc cgtcctcgcc
     1081 atggccgagg tggtcgtaat gggctccgat gaccacggtg gtcggcgcgc cgttattcag
     1141 gtagcccacc acgttgtggc ctgtccgctt tttttcggtg aagccgacct tgagccggat
     1201 gtccagggag gcggcttcgt ccttcaggta ttttcgttta gcctcgcggg tgatatagac
     1261 caccgggatg gtaacgggtt cgggcttatc gtgtggatcg aaggccaggt tatcggcgat
     1321 gcgcgaagag ttgtagagga tgagggccgt ggcgcctttt ttggtgcagg cagccgcttt
     1381 ggcgcggatg gcgccgggca gatcgaaatg cgggttcccg gcattggcct ccagcagctc
     1441 gcgcagatcg aagaaccagg gtactccgct ctcctgtaag gcgatggccg gggagccgga
     1501 aacggccgtg gcggcgctga gggccagggg gaagaattct ttttccaaca ggaacggatg
     1561 atcgttgacg acaaagaagg catcgcggct cacttcccgt ccctgatcga tctcgaaggt
     1621 ctggagccag ccattattgt cgccccttgg ttgtatgcct gcctgggcca gtgcgctgct
     1681 gatatagtcg cttgccgcct tttctccagg ggtgcccgtt ctcctgcctt ccaaatgggg
     1741 gtcggcaaga tagcggatat gggcctgcag attggaaagg gtggttttgt cggtcttttt
     1801 tagcttttgt gcggaggcgg gggttatcag acagggaagg accaggatag gtgttaaaaa
     1861 tttaatacgg gagatcatat aagccggttt aacataacga tctgtggtac aaagggaaac
     1921 ttggttctgg gccttccaaa ggtaagatgg atttattttc tacactcacc ttttttcctt
     1981 tagttttgca gattggcctt tccacaacag accccgtaac cgggccgtgg aaaggggatt
     2041 tgttaaaaca gcatatggcg ttaccgcacc ttattaagta tgtatatacc aacgggaccg
     2101 atgaagtgat tcggcgggga aagaaaattc atgcaatcgg ttatgtagag ctgatcgaat
     2161 acgacgagtt attcgaatcc gtcacctt
//
LOCUS       DATGFG010001167         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_896986, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001167 DATGFG010000000
VERSION     DATGFG010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896986"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..501
                     /locus_tag="VKQ52_22135"
     CDS             118..501
                     /locus_tag="VKQ52_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89975.1"
                     /translation="MMNRKFLLVTLLVSGVVLYLAGCSKESEDKLAGTTTCDTTSVSY
                     SLQIVPILQNNCYACHGQGNTAGSGGINLSTYTALKVYAGNGYLVGNVTHAPGYIGMP
                     YGLPALPSCEQNTIVAWVHQGALNN"
     gene            525..1073
                     /locus_tag="VKQ52_22140"
     CDS             525..1073
                     /locus_tag="VKQ52_22140"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLZ89976.1"
                     /translation="MKFVLIASLFFSALIRPLAGHSQLYSTRTGFIGFYSKTSLEDII
                     AENNQVYAIIDGGKKNLAFSLLVKGFIFRKELMQEHFNENYVESDKYPKANFAGAYTG
                     DVPLNKDGSYNVTVKGNLTLHNVTKSVETPATIEVKNGHLLGQAEFKLKPEDFNINIP
                     SLVRDKIDKAITVKVNIDCTGK"
     gene            1092..2003
                     /locus_tag="VKQ52_22145"
     CDS             1092..2003
                     /locus_tag="VKQ52_22145"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HLZ89977.1"
                     /translation="MVTKKILFTLLSLAVINLAAVNRLNAQDSTLLHMLNDSMTANKG
                     KSYVTGTFKAEHIVNTQTVESPANGNLNFVIQHRFGQLNSGAYNFFGLDNATLRLGLD
                     YGLTDDLSVGIGRSSYLKTFDGYLKYKLLRQTDGPQMPVTVDILGTITNWTQKDDTKL
                     YLNANRRTSYSTELLIARKFSRNLSLQVTPTYLHYNLVPTVNDKNDVFALGLGGRMKI
                     TRRMSINAEYDIVPKNQVVSTTVHNSLSFGWDIETGGHVFQLVFSNSQSMLATQYLTQ
                     TTGTWGKGDIYFGFNISRNFNLKKTRH"
     gene            2000..>2821
                     /locus_tag="VKQ52_22150"
     CDS             2000..>2821
                     /locus_tag="VKQ52_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89978.1"
                     /translation="MKIIRKINAVLTTAERRRLNGLMILDILASIADIGFLALLLFVI
                     HLYADAPAGKTFSFLPGWFTDRSSLWPIGIFFLLFTLKNALSFRIYGAQARFRYGVAS
                     RISHRNLMLYLEGDYRQYAQTDSAKHVLKIAQQPIEFCQFVLAGYQQGVTEGTLIGMT
                     VLAVLLFNAKLFLLLLLLLVPPVVITALLTKRKLHAARTYIKSSRDIMWQHLQESIAS
                     YVESSLYDKKKFFSGRYGDAQTILNRHLAIVQSLQGAPSRLAEIFAVFGLLALIAI"
BASE COUNT          722 a          889 c          630 g          580 t
ORIGIN      
        1 gaaccaaaaa ccggcgataa ggtcaccctg aaaggaaggt gcaccggctt cctgatggat
       61 gtcaatctcg ccgattgcgt acccgataac taaacatctt acataaacat agcaacaatg
      121 atgaatcgta aatttctcct ggtaacgctg ttggtcagcg gcgtcgtact ctatctcgcc
      181 ggctgctcga aagaaagcga ggacaaactt gccggaacaa ccacctgcga tacgacaagc
      241 gtgagttact ccctgcagat cgtccccatc ctgcaaaata actgctacgc ctgccacggc
      301 cagggcaaca cagccggcag cggcggcatc aaccttagta cctatacagc gctgaaagtc
      361 tatgcaggca acggttacct cgtcggtaat gtcacgcacg ccccgggata catcggcatg
      421 ccctacggac tgcccgcgct accctcctgc gaacaaaata ctatcgttgc ctgggtacac
      481 cagggagcac tcaataattg atcatccatc gtaaaatgac cagtatgaaa ttcgtcctta
      541 tcgcctccct ttttttcagc gcccttatcc gtccgctcgc cggccatagc cagttgtatt
      601 ccacccgcac cggcttcatc ggcttctatt caaaaacatc cctcgaagat atcattgcag
      661 agaataacca ggtgtacgcc atcatcgacg gcggcaagaa gaacctcgcc ttctcgttgt
      721 tggtaaaggg attcattttc cggaaagaac tgatgcagga acacttcaat gagaactacg
      781 tcgaaagtga taaataccca aaagccaatt tcgccggcgc ctacaccggc gacgtccccc
      841 tgaataagga cggttcctat aatgtcaccg taaaaggaaa tctaaccctc cacaacgtga
      901 caaaatccgt cgaaaccccc gccaccatcg aggtaaagaa cggccacctg ttaggtcagg
      961 cggaattcaa gctgaaaccc gaagatttta atatcaacat cccctccctt gtaagggata
     1021 agatcgataa ggcaataacg gttaaagtaa acatcgactg caccggcaaa taacaaaaaa
     1081 actcactgat aatggttacg aaaaaaattc tttttaccct gctaagcctc gcggttatca
     1141 accttgcggc cgtcaaccgg ctcaacgccc aggacagcac cctgctccac atgctgaacg
     1201 attccatgac ggcgaataaa ggcaagagct atgtcaccgg aacattcaag gcagaacata
     1261 tcgtcaatac tcaaaccgtc gaatccccgg ccaacgggaa cctcaacttc gtgatccagc
     1321 accggttcgg acagttgaac agcggggcct acaatttttt cggcctcgac aacgccaccc
     1381 tccgcctggg cctcgactat ggcctcaccg acgacctgtc ggtaggcatc ggccgcagct
     1441 cctatctgaa gaccttcgac ggctacctga aatataaact gctcaggcaa acagacggcc
     1501 cacagatgcc cgtcaccgtg gacatcctcg gtaccatcac caactggacc cagaaagacg
     1561 acacaaaatt gtatctgaac gccaaccggc ggacgtccta ttcgaccgaa ctgctcatcg
     1621 cccgcaagtt cagccggaac ctgtccctgc aggtgacccc gacctacctg cattacaatc
     1681 tcgtaccaac cgtcaacgac aagaacgatg tcttcgccct tggcctcggc ggccggatga
     1741 agatcacccg gaggatgagc atcaacgccg aatatgacat cgttccgaag aaccaggtcg
     1801 tgtccaccac cgtccacaac tcgctttcct ttggctggga tatcgaaacc ggcggccacg
     1861 tcttccaact cgtattcagt aactcccaga gcatgctggc cacccagtac ctcacccaga
     1921 ccaccggcac atggggtaag ggggatatct actttggctt caacatctcg aggaatttca
     1981 accttaaaaa aaccaggcat tgaagatcat ccggaaaata aacgcagtcc tgaccaccgc
     2041 cgaaaggcgg cggttgaacg ggctcatgat cctggatatc ctcgccagta tcgccgacat
     2101 cggcttcctg gccctcctgt tattcgtcat ccacctttat gcagacgccc ctgcggggaa
     2161 aacattctcc ttcctgccgg gctggttcac cgatcgctcc tcgctctggc ccatcggtat
     2221 cttcttcctg ctgttcacgc tcaaaaatgc cctcagtttc cgcatctatg gcgcccaggc
     2281 ccgtttccgc tatggcgtgg cctcccgtat ctcgcaccgc aacctgatgc tgtatctcga
     2341 aggggattac cgccagtatg cccagacgga ctccgcaaaa cacgtcctga agatcgccca
     2401 gcaacccatc gagttctgcc agttcgtgct ggccggctac cagcagggag taaccgaagg
     2461 caccctcatc ggcatgacgg tactggccgt gctcctgttc aatgcaaagc tgttcctcct
     2521 gctcctgctg ctgctcgtgc cgcccgtggt gatcaccgcc ttgctgacaa aaagaaaact
     2581 ccacgccgcc agaacctaca taaaatccag cagggatatc atgtggcaac acctccagga
     2641 gtcgatcgca agctatgtcg agagcagcct gtatgacaaa aagaagttct tctccggccg
     2701 ctatggcgat gcccaaacca tcctcaaccg gcatctggca atcgtgcagt ccctgcaagg
     2761 cgcaccttcc cgtctcgcgg agatcttcgc cgtcttcggt ctgctggcgc ttatcgccat
     2821 c
//
LOCUS       DATGFG010001168         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_255476, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001168 DATGFG010000000
VERSION     DATGFG010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255476"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VKQ52_22155"
     CDS             complement(<1..488)
                     /locus_tag="VKQ52_22155"
                     /inference="COORDINATES: protein motif:HMM:NF014924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HLZ89979.1"
                     /translation="MRSRIIFLFLLVAPLLLRAQSARILINQVGYEQDKTKRAIIQTN
                     TRETIAAFQLIDDSTNKTVYAGKPVYSGAVANWKHWQFYTLDFTACKKPGVYRLQASI
                     GGRATTSYPFVIGKNVFEKATLSDILYYFKGQRSAGLIDQADHHLPLPAAAHNPAVPD
                     GQ"
     gene            complement(635..>2106)
                     /locus_tag="VKQ52_22160"
     CDS             complement(635..>2106)
                     /locus_tag="VKQ52_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89980.1"
                     /translation="AFGGFGGPQPMVETPVGGANILSGIGLSTSASNPRAVACKWQSG
                     EHSYLNDVKFIGGHGGIPRPNTADTARRQSERDWDTQYWSLWVTNGGGGTFKDIWTAN
                     TYATAGAYISHTSTPGRVYALSVEHHVRNEVRFNAVANWKVYALQLEEESRESTECQP
                     MEIQDCHDMTFANLYMFRVIRVNKPYPWSIRRWGGSNLEFLNVHNYSQIIYTTTNTIY
                     DVNTNTEVRPWEFARLFIGPENASPTGTAQLATGFEFAQSPCTDSKGNIYFCDSRLRR
                     ICRWSPATRSTTLIAEYPWQPLSLACDKKDNLLVVFKYVPKPGTGETFTNPPDAAGTS
                     FSGWGNSGFATWAYSIDPNNPDETIHLLPTVAMGSIDPVDHALYPAHRWRDYHDFNTI
                     AIARATQCFLAPDGVTIIPIVYDLARSTALIPAIPGKPATQPMSTINAPCVCRPTAGE
                     ISPISRTLHSGESSPPRRMQREMSILPMARSMSTTRRAT"
BASE COUNT          382 a          485 c          768 g          471 t
ORIGIN      
        1 tcctggccat ccgggacggc gggattatgc gcggcggcgg gcaggggaag gtgatggtcg
       61 gcctgatcga tgagccctgc cgaacgctgg cctttgaaat agtagagaat atccgagagg
      121 gtcgcttttt cgaagacatt cttcccgatg acaaagggat aggatgtcgt ggcacggcca
      181 ccgatagatg cctgtagccg gtagacaccg ggctttttgc aggccgtgaa gtcgagcgta
      241 tagaactgcc aatgcttcca attggcgacg gcgccgctgt agacgggctt gccggcgtag
      301 acggtcttgt tcgtgctgtc atcgataagt tggaaggcgg caatggtctc gcgggtattg
      361 gtctggatga tggcgcgttt ggtcttgtcc tgttcgtagc caacctggtt gatgaggatg
      421 cgggcggact gggcccgaag gagcaggggg gcgacgagca ggaagaggaa gatgatccgg
      481 gatcgcatag gttatttgtt ttgtagttgc agttttgggg tcgggagttg gagctcatag
      541 agggattggt caccggtaaa gtacaggatc ggttcgccgg cgctgccgcc gaagcagaga
      601 ccagtggggc gctcgggggt tttcagggtg tttatcaggt ggccctgcgg gtcgtagaca
      661 tagatctcgc catcggcaat atagacattt cccgctgcat ccgtcgcggt ggcgaactct
      721 ccccgctgtg caaagtacgt gagatcggag agatctcccc tgctgtcggt ctgcatacgc
      781 acggtgcgtt tatcgtactc atcggttgcg tggcgggttt gcccggtatg gcggggatga
      841 gtgcggtcga gcgggccagg tcgtatacga tggggatgat cgtcacgcca tccggggcca
      901 ggaagcattg tgtcgcgcga gcgatggcga tggtgttgaa gtcgtggtag tcccgccagc
      961 gatgggccgg gtagagtgcg tggtcgacgg gatcgatgct gcccatagcc actgtcggaa
     1021 ggagatggat ggtctcgtcg gggttgttgg gatcgatgga gtaggcccag gtggcgaagc
     1081 ccgagttacc ccagccgcta aaggaggtgc cggcggcgtc gggtgggttg gtaaaagttt
     1141 caccggtgcc gggtttgggg acgtatttaa agacgacgag gaggttgtct tttttgtcac
     1201 aggcaagcga gaggggttgc caggggtatt cggcgatgag cgtggtgctg cgggtggcgg
     1261 gtgaccagcg acagatgcgg cggaggcggg agtcgcagaa atagatattg cccttgctgt
     1321 cggtgcaggg gctttgggcg aattcgaaac cggtggcgag ttgtgctgtt ccggttgggc
     1381 tggcattttc cgggccgatg aagaggcggg cgaattccca ggggcggacc tcggtattcg
     1441 tattgacgtc gtagatcgta ttggtggtgg tgtagatgat ctggctataa ttatggacgt
     1501 tgaggaattc gaggttgctg ccaccccagc ggcggatgga ccaggggtag ggtttgttca
     1561 cgcggatgac gcggaacata tagagattgg caaaggtcat gtcgtggcag tcctggatct
     1621 ccatgggctg gcattcggtg gattcgcggc tttcttcttc cagctgcagc gcatagacct
     1681 tccagttggc gacggcattg aagcggactt cattccggac atggtgttcc accgaaagag
     1741 cgtagacccg accaggggtt gaagtatgag aaatataggc gcctgccgtc gcataggtgt
     1801 ttgccgtcca gatgtcttta aacgttccgc cgccgccgtt ggtgacccaa aggctccagt
     1861 attgggtatc ccagtcgcgc tcggactggc gacgcgccgt gtcggccgtg ttgggccggg
     1921 ggatgccgcc atggccgccg atgaatttta cgtcgttcag ataggaatgt tcgccggact
     1981 gccatttgca ggcgacggcg cgggggttac tggcgctggt agagaggccg atgccgctga
     2041 ggatattggc gccgcctaca ggagtctcca ccatgggttg gggtccgccg aagccgccaa
     2101 aggccg
//
LOCUS       DATGFG010001169         8659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_469006, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001169 DATGFG010000000
VERSION     DATGFG010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469006"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VKQ52_22165"
     CDS             <1..787
                     /locus_tag="VKQ52_22165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89981.1"
                     /translation="LFPLPLITDQDHALLISKSNPLSPNLADLSGSAFSGQLRDWLEK
                     AGTVSFYNIGNELSPYWKALKETGVFRFEQGDLYTLQSHLHHNTFLTHNQRTDQVVIE
                     PAGIIIRRNDEPGDTSAIGTTATPSNNPQPVIAKYITTPELSAPDHLLRLFAYRRIMQ
                     ELKGRLPGSYRDDDLDANTPQVQMAQEAGIVSPVSSLVVLEKQSDYDKFNIRQSRNSL
                     ENASLHDKGAVPEPGEWAILVTILGLLTFIRYHRLRRRSTTSQ"
     gene            790..2292
                     /gene="xrtN"
                     /locus_tag="VKQ52_22170"
     CDS             790..2292
                     /gene="xrtN"
                     /locus_tag="VKQ52_22170"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase N"
                     /protein_id="HLZ89982.1"
                     /translation="MNHTLLSSARIPATPAWRRWLRQPVGRGHWRRWPKFLALLYTLL
                     AFAGLYNYLTLVSATTAIGILALLYTPRLEKHPPETFRFGWVALFFIALFFLVPVTTT
                     VYLALGCAACLLLETFHRRIERTVPFVLLLLTPITHYFAGILSFPIRLQLTALAGRLL
                     SLSGLPIAVAGNSITFRDHAFSVDPACDGLHLLVVSAVAALLLMNYYQSHFRRRLRTG
                     SILLLLLLTAALNILANLLRIICLVALAILPGNPLHSALGLLFLLVYILLPLVPAIRW
                     TIRRWGTSTPEERTGYRPTRSRALLLGNLLIGTGVLLLVLLHFRMERTVSTGLATVDS
                     IPGYKIKEVEGKVLQLDNVRSLVYIKPIPGFYYTDHTPTICWQGSGYTFSSLQETTTE
                     NTRIFEGILEPQHPSTSAFGFVDGQDSARSGDHRQLYTAWWYDNGIRQTTGPIDWRWD
                     LIRGAPPYSVINITTATREELAAELALALKTHPFRRLLGRPSTQSRVKIW"
     gene            2506..2880
                     /locus_tag="VKQ52_22175"
     CDS             2506..2880
                     /locus_tag="VKQ52_22175"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLZ89983.1"
                     /translation="MTERKTSSTNYRNQSFLEEKCSLNELLDLVSKRWFSDVLFCIQE
                     GNNRFKLIKDDLKFISDTILSDRLKLLERYSLITRTDFDEIPPKVEYSLTDKGEELAG
                     LLEQMCEFSDILMSEVVARRAN"
     gene            complement(2877..3986)
                     /locus_tag="VKQ52_22180"
     CDS             complement(2877..3986)
                     /locus_tag="VKQ52_22180"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HLZ89984.1"
                     /translation="MTQPTPSQRLYSLDALRGFDMFWIAGGEEIFHSLAKITGNSFWQ
                     TLSDQFEHPAWNGFHFYDLIFPLFLFIAGVATPYSVGKELEKGTSRQQLLWRVVRRGL
                     ILVLLGIYYNNGAKIHPWADIRYGSVLGRIGLAYMFANIIYLYTKQRGQTIWFAGLLI
                     GYWLLLAFTSAPGFHHGDLTMEGNFASYIDRSIMPGRLYLKIHDPEGLMSTIPAIGTG
                     LLGILAGQLLKNGALSGGAKAGRLALAGVVSLVLALAWNMVFPINKNLWTSSFVLCVG
                     GISLLLLALFYYVIDVRGYKKWAFFFSVIGMNSIFIYLSGHFINWKYMTDGFFAWVGQ
                     LIGDPWNVFAMVWCLLAVKWLVLYLLYRKKIFLRV"
     gene            4030..4941
                     /gene="bla"
                     /locus_tag="VKQ52_22185"
     CDS             4030..4941
                     /gene="bla"
                     /locus_tag="VKQ52_22185"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HLZ89985.1"
                     /translation="MQRKEVLSKSFLPAAILLFLCLTTRGQSALQDSIRNIARQAKGM
                     VGIAWKISGDPSYEGINTSAHLPMQSVYKFHLALYFLDKADKGQYTPDQPIPIDPTDW
                     QPKIYSPLHEQYKTPPSSLPLQDLLAAIIINSDNIACDILFRAAGGPKPVDAYIKSLG
                     ITDISIAATEAEMHHSWPVQYTNWTTPAAMLTLLEKFDAGKILSPASTRLLLNWMSAS
                     PRLSNRIKGLLPTGTPVAHKPGTSDVSPDGIAAATNDVGIITLPNKKHLLIAAFVSDA
                     KAGEATREWVIARSAQLIFQAGTTATP"
     gene            5086..5916
                     /locus_tag="VKQ52_22190"
     CDS             5086..5916
                     /locus_tag="VKQ52_22190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLZ89986.1"
                     /translation="MVNIQNLHFAYRRRPVFKGLDLELQPGHIYGLFGRNGTGKSTLL
                     RNIAGFLFPQQGHIEALGFTPGKRQPAFLGKLFHIPEDFQLPDITLARWIQYMSPFYP
                     DFNPQQFEHYTREFAIPTFSGLRELSYGQQKKILISLALATNAPLLLMDEPTNGLDIH
                     SKSQFRKVIAGALDEEKCILISTHQIKDLENLIDNITILEEGRIIFNQPMNRIAETLL
                     FKLSFDAEETAQALYSESSLRGHAIILPNTNGEDSRPDLEMLYKAVLLNGKTINALFP
                     "
     gene            5913..6773
                     /locus_tag="VKQ52_22195"
     CDS             5913..6773
                     /locus_tag="VKQ52_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89987.1"
                     /translation="MTTAPTNQLFSFTRWRLLLAKHWIENRRRYILSLLAIGGLLTIW
                     CGFLIIMDAYAPLDVNMQFAAYFVGLFLTGALYSSMLFADLGTKKEALPWLSLPASHL
                     EKLLCALLFGTFLFFIAYTLVFYLVDIPMVEWANSILRNHPRNWPNTNIRIPPVSIYN
                     IFTGDGAPIPEKEDHLFLSFYFAVQAAFLLGSIYFPRYAFIKTVVAVVLFTLGFIVFQ
                     RGVIYPLLPTGWYNNFFRWSTDLYESGPPKSEVRLPLPIENLLTLLGQIGLPPFFWFV
                     TYIRLKEKEV"
     gene            6777..7157
                     /locus_tag="VKQ52_22200"
     CDS             6777..7157
                     /locus_tag="VKQ52_22200"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLZ89988.1"
                     /translation="MQFRETQSIYLQIADYVCEKVLLKEWPPGERIPSVRELAVQLEV
                     NPNTIMRTYEFLQQEKIIFNQRGIGFFTAGDAIEQAARYRRDLFIEKDLPHVFRSLFL
                     LGMDPEELKPLYQQFKKQMRNKNS"
     gene            7161..7964
                     /locus_tag="VKQ52_22205"
     CDS             7161..7964
                     /locus_tag="VKQ52_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89989.1"
                     /translation="MKTSNKLILAFILAAFLVAFTIHAVLYTEYRKGHFVTKEKMHTE
                     AFIRYNLPTPRVISIDGTIWVNLIPADHFALELPHKNEDPDAGMFQPTPAFRLKGAPP
                     SNQAVAFQQRGDTLFVFGNVSIPIHRPFSGFSYRRNLPQVNIYGSATGDLLLNHGQFY
                     LQGAPYPAHAHSARLTISNSTLWIGMQYENLHHPPAEFFDSLDIQAVNSFIVLNTPAM
                     IDHLTMELKDSSTINDRYSNLMHSIIRSTPDSRVEFPADNLKRNQIIIQ"
     gene            7975..>8659
                     /locus_tag="VKQ52_22210"
     CDS             7975..>8659
                     /locus_tag="VKQ52_22210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLZ89990.1"
                     /translation="MQLTIRNLSKTYPNGVRALHDVSLTLDNGMFGLLGPNGAGKSSL
                     MRTIATLQEADSGSITLDDLDVLQNKTRVRQLLGYLPQEFGVYPRISAEQMLDHVARL
                     KGVDRAGERKDLVDGLLNKVNLYKDKKKRLGTYSGGMKQRWGIAQALIGNPKLIIVDE
                     PTAGLDPAERNRFYNLLSHLGEQTIVILSTHIVEDVSTLCSRFAIICLGEVLYSGEPD
                     AAVTSLDGKV"
BASE COUNT         2067 a         3087 c         1832 g         1673 t
ORIGIN      
        1 tctcttcccg ctaccgctca tcaccgacca ggaccacgcc ctcctcatca gcaaaagcaa
       61 ccccctttca cccaacctgg ccgacctcag cggcagcgcg ttctccgggc aattacggga
      121 ctggctggaa aaagccggaa cggtaagctt ctacaacatc ggcaacgaac tgtcccccta
      181 ctggaaagca ttgaaggaaa ccggcgtctt ccgcttcgag caaggcgatc tctataccct
      241 gcaaagccac ctgcatcaca ataccttcct cacccataac cagcgaacag accaggttgt
      301 catcgaaccc gccggcatca tcatccgccg caacgacgaa ccaggggaca catcggcgat
      361 cggcacaacc gcgacaccct ccaacaaccc ccaacccgtc atcgccaaat acattaccac
      421 gccggaactc tccgcccccg accacctcct ccggctcttc gcctaccgcc ggatcatgca
      481 ggagctaaaa ggccggctcc ccggctccta tcgcgacgac gacctggacg ccaacacccc
      541 acaggtgcag atggcgcagg aagccgggat cgtctcgccc gtatccagcc tcgtcgtgct
      601 ggaaaaacaa tccgactacg acaagttcaa tatccgccaa agcaggaaca gcctggaaaa
      661 tgccagcctc catgacaaag gcgccgtacc ggaacccggc gaatgggcca tcctggtcac
      721 catcctcggc ctgctcacct ttatccggta tcatcgcctg cgtcgccgtt ccaccacaag
      781 ccaataacca tgaaccatac cctcctatcc tctgcccgga ttccggcgac gcctgcctgg
      841 cgccgctggc tccggcagcc ggtcggccgc ggccactggc gacgctggcc caaattcctc
      901 gccctcctct acaccctgct ggctttcgcc ggtttataca actatctcac cctcgtctcg
      961 gccacgacgg ccatcggcat cctcgccctc ctgtataccc cccggctcga aaaacatccc
     1021 cccgaaacct tccggtttgg ctgggtggcg ctgttcttca tcgccctgtt cttcctggtg
     1081 ccggtaacga caaccgtcta cctcgcactg ggctgcgccg cctgcctgct gctggagact
     1141 ttccaccggc gcatcgaacg caccgtcccc ttcgtcctgc tgctcctgac acccatcacg
     1201 cattatttcg ccggaatact cagcttcccc atccggctgc agctcaccgc cctcgccggc
     1261 cggctgctca gcctgtccgg cctgccgata gccgtggccg gcaattccat caccttccgc
     1321 gatcacgcct tctccgtaga ccccgcctgc gacggcctcc acctgctcgt ggtctccgcg
     1381 gtcgccgccc tgctgctgat gaactattat cagtcgcatt tccggcgacg cctgcgcacc
     1441 ggctccatcc tgctcctgct gttgctgacc gccgccctga atatcctcgc caacctgctg
     1501 cgcatcatct gcctcgtggc cctggccatc ctccccggca atccgctgca cagcgccctc
     1561 ggcctgctct tcctgctggt ctatatcctc ctccccctgg tacccgccat ccgctggacc
     1621 atccgccgct ggggtacatc cacaccggag gaacgcaccg gctaccgccc cacccgcagc
     1681 agggccctcc tgctgggcaa ccttctcatc ggcacaggcg tcctgctcct cgtcctgctg
     1741 cattttcgca tggaaagaac ggtatccacc ggcctggcga cggtcgacag cattcccggc
     1801 tataaaataa aggaagtgga aggcaaggtc ctgcaactgg ataacgtcag gtccctggtc
     1861 tatatcaagc cgatccccgg cttctattac accgaccata ccccaaccat ctgctggcag
     1921 ggcagcggct atacattcag ttccctgcag gaaactacta ctgaaaacac ccgcatcttc
     1981 gaaggcatcc tggaaccaca acatccgtcc acttcggcct tcggcttcgt ggacggacaa
     2041 gactccgccc ggtccgggga ccaccggcaa ctctacaccg cctggtggta tgacaacggt
     2101 atccgccaga ccaccggtcc catcgactgg cgctgggatc tgatccgcgg cgctcccccc
     2161 tattccgtca taaatatcac caccgccacc cgcgaagaac tggcggccga actggccctt
     2221 gccctgaaaa cccacccctt ccgccgcctg ctcggaagac cttcaaccca atccagggtt
     2281 aaaatatggt aatagttggc taatttccgg ccaaccatga gccaaaaaac tacgttcttt
     2341 gatgatggct aagtttaaac ttattaacac acttgctcaa aaattagtat agccggtcat
     2401 ttccaccctt tattggtgga atagtgtaga gctaaaaaat tatgtttgtg taatatgttt
     2461 gattgcctgc cagacccgac cgttcaacca taaaaatact gaacaatgac agagcgaaaa
     2521 acaagttcca ccaattatcg caaccaatcc ttcctggaag aaaaatgcag cctgaatgaa
     2581 ctactcgatc tcgtaagcaa gagatggttc agcgacgtcc tgttctgcat ccaggagggt
     2641 aacaatcgct tcaaactcat aaaggatgat ctgaagttca tcagcgatac catcctgtcc
     2701 gacaggctga aactcctcga acggtatagc ctgatcaccc gcaccgactt cgatgagatc
     2761 cccccgaaag tggaatattc cctcacggac aaaggagaag agctcgcagg gctgctcgag
     2821 cagatgtgcg agttcagcga catcctcatg tcggaagtcg tcgcccggag agcaaactaa
     2881 acccgcagga agatcttctt gcggtagagg agataaagga cgagccactt caccgccagc
     2941 agacaccaaa ccatggcaaa gacattccat ggatcaccga tcaattgccc aacccaggca
     3001 aaaaatccgt cggtcatata tttccagtta ataaaatgac ccgacaggta gataaagatg
     3061 gagttcatcc cgatcaccga gaagaaaaaa gcccatttct tatacccccg tacatcgatg
     3121 acataataaa agagcgccag taacaaaagg ctgatcccgc cgacacacaa cacaaaagag
     3181 ctcgtccaca ggttcttatt gatcgggaaa accatattcc acgccaacgc cagcaccagg
     3241 gaaacaaccc cggccagcgc cagccgcccc gccttggccc cgcccgacaa cgccccgttc
     3301 ttcagcaact gccccgccag aatacccaac agacccgttc cgatcgccgg gatggtggac
     3361 ataagcccct ccgggtcatg gatctttaaa tacaacctcc caggcatgat cgacctgtcg
     3421 atatacgatg caaaattccc ttccatcgtc aggtcaccat gatggaaacc cggcgcggag
     3481 gtaaacgcta acagtaacca ataaccgata agcaacccgg caaaccagat cgtctgcccc
     3541 cgctgcttcg tatacaggta gatgatattg gcgaacatat acgccaaccc gatccgcccc
     3601 agcacactcc cataccggat atccgcccag gggtggatct tcgccccgtt gttgtagtaa
     3661 atacccaaca acaccaggat caacccccga cgcacaaccc gccacaacag ttgctgccgc
     3721 gacgtcccct tctccagctc cttgccgacg gaatagggcg tcgccacacc cgcgataaaa
     3781 aggaagagcg gaaagatcag gtcatagaaa tggaaaccgt tccaggccgg atgctcgaac
     3841 tggtcggata atgtctgcca gaaagaatta ccggttattt tggcaaggct gtggaagatc
     3901 tcctctccgc ccgcgatcca gaacatatca aaaccccgga gggcatccag cgaatacaat
     3961 cgctgcgaag gtgtgggttg ggtcataata gccatgaata tagtaaaatt attgcctaca
     4021 tttaagccca tgcaacgaaa agaagtatta tccaaatcct ttctccccgc cgccatcctc
     4081 ctcttcctct gcctgaccac ccggggccag tccgccctgc aggattctat ccgcaacatc
     4141 gcccgccagg cgaagggaat ggtaggcatc gcctggaaaa tatccggcga cccatcctac
     4201 gaaggcatca acaccagcgc ccacctgccc atgcaaagcg tatacaaatt ccatctcgcc
     4261 ctttacttcc tggataaagc agacaaaggc cagtacaccc ccgaccaacc catccccatc
     4321 gatcccacgg actggcaacc gaaaatctat agcccactcc acgaacaata caagaccccg
     4381 ccctcctcgc ttccgttaca ggacctgctt gcggcaatca tcatcaacag cgataacata
     4441 gcctgcgata tcctcttccg cgccgccggc ggaccgaaac cagtagacgc ttatataaag
     4501 agcctcggca tcaccgatat ctccatcgcc gctaccgaag ccgaaatgca tcactcctgg
     4561 cccgtccagt acaccaactg gaccaccccc gccgccatgc tcaccctcct cgaaaaattc
     4621 gacgcgggca aaatactctc ccctgccagt acccgactcc tgctgaactg gatgagcgcg
     4681 tccccccgcc tctccaaccg gatcaaagga ctcctgccca ccggcacccc cgtagcccac
     4741 aaacccggca catcggacgt ttcccccgac ggcatcgccg cggccaccaa tgacgtcggc
     4801 atcatcacgc tgcccaataa aaaacacctc ctcatcgccg ccttcgtctc cgacgcaaaa
     4861 gccggcgagg ccacccgtga atgggtcatc gcccgtagcg cccagctgat cttccaggcc
     4921 gggaccaccg ccacgccata atatccccgc cggccgcttc cgcgcctcct ccaccaaatt
     4981 gtcccccgac gcgtaaacgc cggcccgaaa atttttcttc aaaaaaattt gcccccttca
     5041 aaaaatcttc acaacttagt gtactatttc actagtacac ctccaatggt caacatccaa
     5101 aaccttcact tcgcctaccg ccgccgcccc gtattcaagg gcctcgacct cgagctccaa
     5161 cccggtcaca tctatggcct gttcggccgc aacggcacag gcaaatccac ccttctccgc
     5221 aacatcgccg gcttcctctt tccccagcag ggccatatcg aagccctggg ttttaccccc
     5281 ggcaaacgcc agcccgcctt cctggggaaa ctctttcata tacccgaaga cttccaactg
     5341 cccgacatca ccctcgcccg ctggatacag tacatgtcac ccttctaccc ggacttcaac
     5401 ccgcaacaat tcgaacacta tacccgcgaa ttcgccatcc ccaccttctc cgggttgcgc
     5461 gaactgagct atggccagca aaaaaagatc ctcatcagcc tggccctcgc caccaacgcc
     5521 ccgctcctgc tgatggacga acccaccaac ggcctcgaca tccatagcaa gagccagttc
     5581 aggaaagtga tcgccggcgc cctcgacgaa gagaaatgca tcctgatcag cacccaccag
     5641 ataaaagatc tggagaatct tatcgataat atcaccatcc tcgaagaagg caggatcatc
     5701 ttcaaccagc ccatgaaccg catcgccgag accctgctgt tcaaactctc cttcgacgcc
     5761 gaagaaacgg cacaggccct gtacagcgaa tcatcactca gaggccacgc catcatcctt
     5821 cccaatacca acggggagga tagccgtccc gacctggaaa tgctctacaa agccgtcctc
     5881 ctcaacggca aaaccataaa cgccctgttc ccatgacaac cgcaccgaca aaccaactct
     5941 tcagctttac ccggtggcgc ctgctcttgg ccaaacactg gatcgagaac cggcgtcgtt
     6001 atatcctgtc cctgctcgcc atcggtggac tgctgaccat ctggtgcggc tttctcatca
     6061 tcatggatgc ctatgcccct ctcgatgtca acatgcagtt tgccgcctat ttcgtcggtc
     6121 tcttcctcac cggcgctctc tattccagta tgcttttcgc cgacctgggc acaaaaaagg
     6181 aagccctccc ctggctgtcc ctccccgcct cccacctgga aaaactgctc tgcgccctgc
     6241 tcttcggcac tttcctcttc ttcatcgcct ataccctcgt gttctacctg gtggacatcc
     6301 ccatggtcga atgggcgaac agcatcctcc gcaaccatcc ccgtaactgg cccaacacca
     6361 acatacgcat cccgcccgta tccatttaca acatcttcac cggcgatggc gcccccatcc
     6421 cggaaaagga agatcattta ttcctgagct tttatttcgc cgttcaggcc gccttcctgc
     6481 tcgggtccat ctatttcccc cgctatgcct tcataaagac cgtcgtcgcg gtcgtgctgt
     6541 tcacactggg ttttatcgtt tttcaacgtg gcgtcattta ccccctgttg cccaccggct
     6601 ggtacaacaa tttcttccgc tggtcaaccg atctgtatga atcgggacca cccaaaagcg
     6661 aggtccggct gcccctgccc attgaaaatc tgctcaccct cctgggccaa ataggactgc
     6721 cccccttctt ctggttcgtc acctatatcc ggttaaaaga aaaagaagtc taacccatgc
     6781 agttcagaga aacccagagc atctacctgc agatcgccga ttatgtctgc gaaaaggtcc
     6841 tcctgaagga atggccgccg ggcgaacgca tcccctccgt ccgcgaactc gccgtgcaac
     6901 tggaagtaaa ccccaatacg atcatgcgta catatgaatt cctgcaacag gaaaagatca
     6961 tcttcaatca acggggtatc ggcttcttca ccgccggcga cgcgatcgaa caggcggccc
     7021 gctaccgccg ggatctcttc atcgaaaaag acctgcccca tgtcttccgc agcctcttcc
     7081 tgctcggcat ggacccggaa gaactaaagc ccctgtacca gcagttcaaa aaacaaatgc
     7141 gtaacaaaaa cagctagacc atgaagacca gcaataaatt gatccttgcc tttatcctgg
     7201 cagccttcct ggtggcgttc accatccacg cagtgctgta taccgaatac cgcaagggcc
     7261 acttcgtgac aaaggagaaa atgcacacgg aagccttcat acgatataac ctgcccacac
     7321 cgcgggtcat ttccatcgac ggtaccatct gggtcaacct catcccagcc gaccattttg
     7381 ccctcgagct tcctcacaag aacgaggacc ccgatgccgg gatgttccaa cccactcctg
     7441 ccttcaggct aaaaggggct cccccctcaa accaggccgt cgcctttcag cagcgcggcg
     7501 acacgctttt cgtcttcggc aacgtctcca tccccattca ccggcccttc tccggctttt
     7561 cctaccgccg caacctccca caggtgaaca tctacggctc cgccaccggt gatctcctcc
     7621 tcaaccatgg acagttctac ctgcagggtg caccctatcc ggcccacgcc cacagcgccc
     7681 ggcttaccat cagcaattcc accctttgga tcggcatgca gtacgagaac cttcaccacc
     7741 cccccgccga attcttcgat agcctcgata tccaagcagt aaacagcttc atcgtgctga
     7801 atacacccgc catgatcgat cacctcacca tggagttaaa ggacagctcg accatcaacg
     7861 accggtactc caacctcatg cattccatta tccgctccac ccctgactcc cgggtcgaat
     7921 tccccgcgga taacctgaaa cggaaccaaa tcatcattca ataaaaagct tcccatgcaa
     7981 ctcaccatcc gcaatctgtc caagacctat cccaacgggg tccgcgcact ccacgatgtc
     8041 tccctcaccc tcgacaacgg tatgttcggc ctcctcgggc caaatggcgc aggcaaatcc
     8101 tccctgatgc gaaccatcgc taccctccag gaagccgaca gcggctccat caccctcgat
     8161 gatctcgacg tcctccaaaa caagacccgc gtccggcaat tactcggcta cctgccccag
     8221 gaattcggcg tctacccccg catctccgcc gaacaaatgc tcgaccacgt cgccaggctc
     8281 aaaggcgtcg accgcgccgg cgaaagaaaa gacctcgtcg acggcctgct gaacaaggtc
     8341 aacctctata aggacaagaa aaaacgactc ggaacctact ccggcggcat gaaacaacgc
     8401 tggggcatcg cccaggccct gatcggcaat ccaaaactca tcatcgtcga cgaacccacc
     8461 gccggcctcg accccgcgga acgcaaccgg ttctacaacc tcctcagcca cctcggtgaa
     8521 cagaccatcg tcatcctgtc cacccatatc gtcgaagacg tcagcaccct ctgctcccgg
     8581 ttcgccatca tctgcctcgg cgaagtcctc tacagcggtg aacccgacgc cgctgtgaca
     8641 tccctggatg gtaaggtct
//
LOCUS       DATGFG010001170         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_643334, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001170 DATGFG010000000
VERSION     DATGFG010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643334"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VKQ52_22215"
     CDS             <1..1038
                     /locus_tag="VKQ52_22215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89991.1"
                     /translation="HLQLISSWSNLSLELYSWYIRNGVARNEKDEALVQEFFEKNLPE
                     DLSQCEGFYERLYQYQSFCWYAFIRQDFLLYYRYTQKWVDLFEKEPFMIGIETSHYIK
                     GMHNLLGAHFDLLNDQKFAETLKQFEAFSESDIVRNNVNNRIQTFVYLYISKLNQHFI
                     YGTFTEGLSLVPYIEEKLKEYWIYLDRHRILVFYYKIACLYFGSGDYGKTIDYLNKII
                     NWKVDLRTDLQCYSRLLHLIAHYELGNFDLLEYLTKSVYRFMAKMENLSLVEEEIFRF
                     LRKSFNLSPGQLKPEFAKLLEKLKKYEKNRFETRAFVYLDIISWLESKLEEVPVQEII
                     RQKYVSGKRRG"
     gene            complement(1085..1513)
                     /locus_tag="VKQ52_22220"
     CDS             complement(1085..1513)
                     /locus_tag="VKQ52_22220"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLZ89992.1"
                     /translation="MKVFVDANILVATVNKEYPLFPYTSRIMTLTTWKDFTIYTTPVC
                     LAIAFYFAEKRYGTSSAKKRLQLLSFNVAIAPTTDQTVQSAFGDPAVMDFEDGLEYFA
                     ALEAGCTCIVTEDKDDFHFSRIEVLSSMEFVERYILSRRF"
     gene            complement(1510..1767)
                     /locus_tag="VKQ52_22225"
     CDS             complement(1510..1767)
                     /locus_tag="VKQ52_22225"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HLZ89993.1"
                     /translation="MDNKVTLSFDKEVIRLAKEYAASQNISLSRLVELLLRKTTMEQY
                     RSLEEIPIASWVREVSEGPMEYRARKRSRKDSKDEFFSSKK"
     gene            2069..>2806
                     /locus_tag="VKQ52_22230"
     CDS             2069..>2806
                     /locus_tag="VKQ52_22230"
                     /inference="COORDINATES: protein motif:HMM:NF016858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ89994.1"
                     /translation="MAIKKENRPRATFSKITIGLASPDSILERSYGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECACGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWYFKSLPNKIGYLLGMSSKKLESIIYYERYVVIQPGIRTDKGQNYGDLLT
                     EEEYLEILDTLPKDNQYLPDEDAAKFIAKMGAEAVHDLLERIELDQLSFDLRNAAANE
                     TSQQRKADALKRLSVVEAFRDANSRITN"
BASE COUNT          681 a          584 c          833 g          708 t
ORIGIN      
        1 catctgcaat tgatcagcag ctggtcgaac ctttcccttg agctatatag ttggtatatc
       61 cgcaatgggg tggcgcggaa tgagaaggac gaggcattgg tgcaggagtt cttcgagaag
      121 aacctgccgg aggacctcag ccagtgcgag gggttttatg agcggcttta ccaataccag
      181 agtttttgct ggtatgcgtt tatccgccag gatttcctgt tgtattaccg ctatacccag
      241 aaatgggtag acctttttga aaaggagccc tttatgatcg ggatcgagac ctcgcattat
      301 atcaagggga tgcacaacct gctgggggcg catttcgatc tgttgaacga ccagaagttt
      361 gcggagacgc tgaagcagtt tgaggcattt tccgaatcgg acattgtgcg aaacaacgtg
      421 aacaacagga tacagacctt tgtttacctg tatatctcca agcttaacca gcattttatt
      481 tacggtacgt tcacggaggg gttgtcgctt gtcccctaca tcgaagagaa gttgaaggag
      541 tactggatct atcttgaccg gcaccggata ttggttttct attacaagat agcctgcctg
      601 tatttcggga gcggggatta tgggaagacg atcgattacc tgaacaagat catcaattgg
      661 aaggtggacc tgcggaccga tctgcagtgt tattcgcggc ttttgcacct gatcgcccac
      721 tatgaactgg ggaatttcga cctgttggag tatttgacga agtcggtgta ccggttcatg
      781 gcgaagatgg agaacctgag tcttgtggag gaggagatct tccggttttt gcggaagtcg
      841 ttcaatcttt cgccggggca gctgaagcct gagtttgcga agctgctgga gaagctgaag
      901 aagtatgaga agaaccggtt cgagacgcgg gcctttgtgt acctggatat catttcgtgg
      961 ctggagagca agctggagga ggtgccggtg caggagatca tacgtcagaa gtatgtgagt
     1021 gggaaaagac gcgggtagca gctcggggcc gaaggccaga cgttggcgaa ggcgcgggta
     1081 ggggctagaa gcggcgtgaa agaatgtaac gttcgacgaa ttccattgat gaaagcactt
     1141 cgatccggga gaagtggaaa tcatccttgt cttccgtcac gatgcaggtg caccctgctt
     1201 cgagtgcggc gaaatattcg aggccatcct cgaaatccat gacggccgga tcgccgaagg
     1261 cggattgaac cgtctggtca gttgttgggg cgatggcgac attgaaagac agcaattgga
     1321 gtcttttttt ggccgaggac gttccatatc ttttttctgc aaaataaaag gcgatggcaa
     1381 ggcataccgg ggtggtataa atggtaaaat ctttccaggt ggtcagggtc ataatgcggg
     1441 atgtataagg aaagagcggg tattccttat tgacggtggc gaccaggata tttgcgtcga
     1501 cgaaaacttt cattttttgg aagagaagaa ttcgtctttg ctgtccttgc ggcttctttt
     1561 gcgggcgcgg tattccattg gcccttccga aacctcccgt acccagctcg caatgggaat
     1621 ctcttccagt gaacgatact gttccatggt tgttttacgg agaaggagct cgaccagccg
     1681 ggacaggctg atattttggc tggcggcgta ttccttggcg aggcggatga cctctttgtc
     1741 gaaactgagt gtcactttgt tgtccatata ggtaaagttt ttgggtgtgt gcgtgggtgt
     1801 tcgtgtgtgt gtgtgtgtgt aaaggtaagg ggaaaaaagt aactttgtac gtagggggat
     1861 ttgggattgt acgtataaaa gcatcggaat gcctgcaagg gcgtggaaaa tattattttt
     1921 tagtgtaaaa atcgtacctt tgccctcctt gatttggacc cacgagcata ttttgagttg
     1981 tttggatagc cgcccggagt aatactgggt agggtatctt tttgatatac aatgggttag
     2041 aacgagtcta aaaaaagcat attgcaatat ggctattaaa aaagaaaatc gccccagagc
     2101 cactttttca aaaatcacga tcggtcttgc atctcccgac agcatcctgg aaaggagcta
     2161 cggggaggta ttgaagccgg agaccatcaa ctatcgcacg tacaagcccg aaagggatgg
     2221 tttgttctgc gagcggattt ttggtcctgt aaaggattat gagtgtgcct gtggaaagta
     2281 caagcgtatc cgctacaagg gtatcgtttg tgatcgttgc ggggtcgaag tgacggagaa
     2341 gaaggtcagg cgtgagcgga tgggccacat caagctggtg gtgccggtgg ttcacatctg
     2401 gtatttcaaa tcccttccca ataagatcgg ttatttgctg gggatgagct ccaagaagct
     2461 ggagagcatc atctattatg aacgatatgt ggtcattcaa cccggtatcc ggacggacaa
     2521 gggccagaac tatggcgacc tgctgacgga ggaggaatac ctggagatcc tggacaccct
     2581 gcccaaggac aaccagtacc tgccggacga ggacgccgcc aagttcatcg cgaagatggg
     2641 cgccgaagcg gttcatgacc tgctggagcg gatcgagctg gaccagctgt ccttcgatct
     2701 ccgcaatgcg gccgcgaatg agacctcaca gcaacgtaag gccgatgcgc tgaagcgttt
     2761 gagcgtggtg gaggctttcc gtgacgccaa ttcccggatc accaac
//
LOCUS       DATGFG010001171         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1173617, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001171 DATGFG010000000
VERSION     DATGFG010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173617"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VKQ52_22235"
     CDS             <1..319
                     /locus_tag="VKQ52_22235"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLZ89995.1"
                     /translation="PTVGTNVLKVGDLVMNLDSKEVSREGRNIPLTAKEFQLLEYFIR
                     NKNKVVSRIDIAINVWDIDFDTKTNVIDVYVNFLRKKIEKDFSSKLIYTQVGMGYVLK
                     ETP"
     gene            366..1730
                     /locus_tag="VKQ52_22240"
     CDS             366..1730
                     /locus_tag="VKQ52_22240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLZ89996.1"
                     /translation="MLLFTLLVTAIISLLTSSVYYFAKLERQQVFEKRLKARATYNTQ
                     LYALMGDSGLHLLRRIDSVSLIGATVSRSIGMYTDQGKVLYQFDMPGTPRLAVTAELL
                     SEVQMKGEKAFFMDNREAVAIHRNAGKRDFIVVVAGHDDDGIERVETLNKILLISLIL
                     GVLLTALVSFLFARQLLRPISQIIREVKQISSFDLSQRVRAGTGQDEMSQLGNTFNDL
                     LARLQEAFAIQRRFISNASHELSTPLTSVSSQVEVTLQKDRSADEYKQVLYSVREDVQ
                     QMRQLTKSLLEIAKTGSQGGIELNEVRVDEVLMKVVGEVKKAGPDYSVELDFGEFPED
                     EKDFVVFGNSELLYIAVKNVVENGCKYSPDKRSEVDLSFAYHKVFVRIVNQGTVIPAE
                     EITQIFQPFYRGAGTGATRGFGLGLAMAERIIALHKGIIHVISDESSGTIFTIELPAI
                     KIFN"
     gene            1741..>2335
                     /locus_tag="VKQ52_22245"
     CDS             1741..>2335
                     /locus_tag="VKQ52_22245"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HLZ89997.1"
                     /translation="MPWTQVTDPCHNLLASAGLAAIPVLVIFWALVIRKMKGYQASLL
                     AIATAFLIAILIYRMPIHLSLLSIGNGALYGLFPICWIILTAVFLFNCTIRSGQFGII
                     RHFMASITADRRVQALLIAFSFGSFLEGTAGFGAPVAITAAMLTGLGFNPLYAAGLCL
                     IANTAPVAFGSIGIPVTVAAQVTGLPEQALSAMIGRTL"
BASE COUNT          525 a          776 c          568 g          466 t
ORIGIN      
        1 gcccaccgtc ggcaccaacg tcctcaaggt cggcgatctg gtcatgaatc tcgatagcaa
       61 agaggtctcc cgcgaaggca gaaatatccc cctgacggca aaagaatttc aactcctgga
      121 atattttatc cggaataaga acaaggtcgt atcccgtatc gacatcgcta tcaatgtctg
      181 ggacatcgac ttcgacacca aaacgaacgt catcgacgta tacgtgaact tcctccggaa
      241 aaagatagaa aaagactttt catcaaagct catctatacc caggtgggga tgggctatgt
      301 gttgaaagaa accccataaa gccgcctccg gccccacata taacgtgaag atccgctata
      361 agataatgtt gctttttaca ctgctggtca cggcgatcat ctccctgctc accagctcgg
      421 tgtattattt tgccaagctg gaacggcaac aggtctttga aaagcggcta aaggcccggg
      481 ccacctacaa cacccagctc tatgcgctca tgggcgacag cggccttcac ctcctgcgcc
      541 gcatcgactc ggtctccctc atcggcgcca cggtctcccg cagtatcggc atgtataccg
      601 atcagggaaa ggtgctctac cagttcgaca tgcccggcac gccccgcctc gccgtcaccg
      661 cggaattgct cagcgaagta cagatgaaag gggaaaaagc ctttttcatg gataaccgcg
      721 aggccgtggc catccaccgc aacgccggga aaagagactt tatcgtcgtc gtggcgggcc
      781 atgacgatga cggcattgaa agagtggaaa ccctgaacaa gatcctcctc atcagcctga
      841 tcctgggcgt gctcctgaca gcgctggtca gcttcctctt cgcccggcaa ttgctgcgcc
      901 ccatctccca gatcatccgc gaagtaaaac agatctcctc cttcgacctc tcccagcgcg
      961 tccgcgccgg cacaggccag gacgaaatga gccagctggg gaatacattt aacgatctgc
     1021 tggcccgcct gcaagaagcc tttgccatcc agcgccggtt tatttccaac gcctcccatg
     1081 aactctccac ccccctcaca tcggtctcca gccaggtgga ggtcaccctc cagaaagacc
     1141 gctccgccga tgaatacaaa caggtgctct actccgtccg cgaagacgtc cagcaaatgc
     1201 ggcaactgac aaaaagcctc ctggagatcg caaagacagg ctcccagggc ggcatcgaac
     1261 taaacgaggt acgcgtggac gaggtgctca tgaaagtggt cggcgaagta aaaaaagccg
     1321 gccccgacta cagcgtggaa ctggattttg gggaattccc ggaggatgaa aaggatttcg
     1381 tggtctttgg caacagcgag ctgctctaca tcgcggtgaa gaacgtggtg gagaacggct
     1441 gcaaatattc ccccgacaaa cggtcggagg tggatcttag ctttgcctat cacaaagtct
     1501 tcgtacgcat cgtcaaccag ggcaccgtta tccccgccga agagatcacc cagatcttcc
     1561 aacccttcta ccggggcgcc ggaacagggg ctacccgcgg cttcggcctg ggcctggcca
     1621 tggccgaacg catcatcgcc ctccacaaag ggatcatcca tgtcatctcc gatgagtcca
     1681 gcggcactat ctttaccatc gaacttcccg cgatcaagat atttaactga ggatccaaca
     1741 atgccctgga cacaagtaac agatccctgc cacaacctgc tggcctccgc cggcctggcc
     1801 gctatcccgg tgctggtcat cttttgggcc ctcgtcatcc ggaaaatgaa gggctaccag
     1861 gccagcctgc tcgccatcgc gaccgccttc ctcatcgcca tcctcatcta ccggatgccc
     1921 atccacctgt ccctgctctc catcggcaac ggcgccctct atggcctctt ccccatctgc
     1981 tggatcatcc tcaccgccgt cttcctcttc aattgcacca tccgcagcgg ccaattcggg
     2041 ataatccgcc acttcatggc ctccatcacc gccgaccggc gggtccaggc cctgctcatc
     2101 gccttttcct tcggctcctt tctcgaaggc acggcaggct tcggcgcccc ggtagccatc
     2161 accgccgcca tgctgacggg actgggcttt aaccccctct atgccgccgg tctctgtctc
     2221 atcgccaaca ccgcacccgt ggcctttggg tccatcggca tccccgtaac ggtagccgct
     2281 caggtgaccg gcctccccga acaagccctc tcggccatga tcggccgaac cctgc
//
LOCUS       DATGFG010001172         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_873947, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001172 DATGFG010000000
VERSION     DATGFG010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873947"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1800
                     /locus_tag="VKQ52_22250"
     CDS             238..1800
                     /locus_tag="VKQ52_22250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLZ89998.1"
                     /translation="MKRKTDNFSKFYNKKNNAAIKEAFRQEKKAAKRERKEAIERHFE
                     EKRKARGYQAPQPGERPGERPAQRGERPTQRPTERTTGKQPIQPPTERAAGKPIKKAP
                     AANASAAAKPPVAKPIPGNSGKATPFKPAPGKATPDAKQTPGAPAKATPGAKQAPGAH
                     HGEQLPLNKYIAHGGVCSRRDAAELIRQGKVTVNGQLVTEPGTKVLPTDTVKFNGKKV
                     TISRNFVYVLLNKPKDYITTTDDPQGRRTVLDLIRPATTERVFPVGRLDRNTSGVLLL
                     TNDGDLAQKLAHPSHEIKKIYHVVLDKPLTKADFEKIIGGSIMLEDGPALVDVLAYAD
                     PKDKTQIGLEIHSGRNRIVRRIFEHLGYDVRGLDRVMYAGLTKKNVQRGKWRLLTEKE
                     VRILKYLNSSFKGSLAPARTAAAAAASAATRTSGASDTAAASRTAAAGAAARTAAAAG
                     KPANTPPTDGRPAKPASPSEDRPDRSARSFGASTPDKRPARAIPPKGRKSAAPPKGRK
                     PYNPKSPRPESD"
     gene            1810..2526
                     /locus_tag="VKQ52_22255"
     CDS             1810..2526
                     /locus_tag="VKQ52_22255"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLZ89999.1"
                     /translation="MHKLDILFENDAFIAVNKPAGLLSIPDREGKQPSLKNILKERYG
                     NIYTVHRLDRDTSGVIVFAKDEATHKYLSGIFEDREVEKLYQGLVQGTLPQKQGSIDL
                     PIMEHPAKPGVMVFNKRGKASLTDYEVIEELSLYSLVQFRIHTGRTHQIRVHMQALGH
                     PIVCDEVYGDAHPILLSSIKRNFKLSKDEEQERPLLARLALHSYLLHFIDATGTAHTL
                     EAPLPKDMRALLQQLRKWKG"
BASE COUNT          702 a         1031 c          594 g          369 t
ORIGIN      
        1 aaggtctggt tccacgacat cttccgcccc gacggcaccc ccttcgatca aaaagagatc
       61 gatctcatcc gtagcctgac catcggcaac taaacccgca actaaaccgg cgcccatccg
      121 caaaaaaagc ctatctttgc cggctgacca cagcctcccg taaggagccg atggagccgc
      181 aggacgcagc tccgccgaag gtcacacatt actgttgtaa aacagcaaac cccgatcatg
      241 aagcgaaaaa cagacaactt cagcaagttc tataacaaaa agaacaacgc tgccatcaag
      301 gaagccttcc gccaggaaaa gaaagccgcg aagcgcgagc gcaaagaagc catcgaacgg
      361 catttcgaag aaaaacgcaa ggcccgcggc tatcaggcgc cacaacccgg cgagcggccc
      421 ggcgagcgac cagcccaacg cggcgagcga ccaacccaac gtcccaccga acgaacaacc
      481 ggcaaacagc ccatccagcc gcccaccgaa cgcgcagccg gcaaaccgat aaaaaaggcc
      541 cccgccgcca atgcatctgc cgcagccaaa ccacccgtcg caaaaccgat ccccggcaac
      601 tccggcaaag caaccccgtt caaacccgcc cccggcaagg caacacccga cgccaaacaa
      661 acacccggcg cacccgccaa agcaacaccc ggcgcaaaac aagcacctgg cgcccaccac
      721 ggcgaacaac ttcccctgaa taaatacatc gcccacggcg gcgtctgctc ccgtcgcgac
      781 gcagcggaac tcatccgcca gggcaaggtc accgtcaacg gccaattggt tacggaaccc
      841 ggcaccaagg tcctgcccac cgacaccgtc aaattcaacg ggaagaaggt caccatctcc
      901 cgcaacttcg tttacgtcct gctgaacaag ccaaaggact acatcaccac caccgatgat
      961 ccccagggcc gcaggaccgt cctcgatctc atccgccccg caaccaccga acgcgtgttc
     1021 cccgtcggcc ggctcgaccg caacacctcc ggcgtactcc tcctgacaaa cgacggcgat
     1081 ctggcccaga aactcgccca ccccagccac gagataaaaa agatctacca cgtcgttctc
     1141 gacaagcccc ttacaaaagc cgacttcgaa aagatcatcg gcggcagcat catgctggaa
     1201 gatggtcccg ccctggtcga tgtcctcgcc tacgccgatc caaaagacaa aacacagatc
     1261 ggcctcgaga tccatagcgg ccgcaaccgc atcgtccgcc gcatcttcga acacctcggc
     1321 tacgacgtcc gcggcctcga tcgcgtcatg tacgcgggcc tcaccaagaa gaacgtccaa
     1381 cgcggcaaat ggcgcctcct cacggaaaaa gaggtccgca tcctgaaata tctcaactcc
     1441 tcgttcaaag gctccctcgc cccggcccgc acagccgccg ccgccgccgc atccgccgca
     1501 actcgcacat ccggcgcatc cgacactgcc gcagcatctc ggacagccgc cgcaggcgca
     1561 gctgcccgaa cagccgccgc cgcgggcaaa ccggcaaaca cccctcccac cgacggccgc
     1621 ccggcaaaac cggcatcccc atccgaggac cgtcctgacc ggtccgcaag atccttcggc
     1681 gcgtctaccc ccgataaacg tcccgcacgc gcgatccccc caaagggccg aaaatccgcc
     1741 gccccaccaa aaggccgcaa accctataac cccaaatccc cccgccccga aagcgattaa
     1801 cgctccccta tgcataaact ggatatcctc ttcgagaacg acgccttcat tgccgtcaac
     1861 aaacccgccg gcctgctcag catccccgac cgcgagggca agcagccgtc cctaaagaac
     1921 atcctcaagg aacgatacgg caacatctac accgtccacc gcctcgaccg cgacaccagc
     1981 ggcgtcatcg tcttcgccaa ggacgaggcg acacataaat acctctccgg catcttcgaa
     2041 gaccgcgaag tggaaaagct ctaccagggc ctcgtccagg gcacgctgcc gcagaaacag
     2101 ggcagcatcg atctgcccat catggaacat cccgcaaaac ccggggtcat ggtcttcaac
     2161 aaacgcggca aggcctcgct caccgactac gaggtcatcg aagagctaag cctgtattcc
     2221 ctcgtgcaat tccgtatcca tacgggccgc acccaccaga tccgggtcca catgcaggca
     2281 ctcggccacc ccatcgtctg cgatgaggtc tatggcgacg cccaccccat cctgctttcc
     2341 tccatcaaac gcaacttcaa actctccaaa gacgaagagc aggaacgccc cctcctcgcc
     2401 cgcctggcgc ttcactccta cctcctccac ttcatcgacg ccaccggcac cgcccatacc
     2461 ctcgaagccc cactgcccaa ggatatgcgc gccctcctgc agcaactccg caaatggaag
     2521 ggctaggccg atagcccaca aacaaaaaaa gaagtaaatt taccagcaca gccgattgta
     2581 tgaacaagcc cctgcatcca tatttggcta tatttttact gagcatctcc ctttcgtcag
     2641 ttgcccaaag cgcaaaaccc tcctccattg attcgacgat ccgctcttct gagacg
//
LOCUS       DATGFG010001173         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_873966, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001173 DATGFG010000000
VERSION     DATGFG010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873966"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VKQ52_22260"
     CDS             complement(<1..704)
                     /locus_tag="VKQ52_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90000.1"
                     /translation="MKNMILCCILFISATMAARSQPTVSSPSPTSQTVCSGTSATFSV
                     SATGTPTLTYQWQVSINNGSTWTAIANNANYSGATTTSLTITSIPTSFNGYSYRCTVT
                     NSLGSSSSTAGVLTVNATPVAQVTGQSIVQCSSEGTGSALAVDPSSNYQWQLSTDAGS
                     TWSNISDGAVYTGTNTYDLTWNLSASMNGYLYRFIISDPSSGCSNTSVGVDTLYVIGS
                     PSVQTLSPVSANICPGT"
     gene            complement(1077..1601)
                     /locus_tag="VKQ52_22265"
     CDS             complement(1077..1601)
                     /locus_tag="VKQ52_22265"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HLZ90001.1"
                     /translation="MAQPIPDPSRPASGQNPTAFLRLIRNPLKARLYLLTKLPSAFFS
                     GLLIPQVDEDQCTVTVPYRWFTRNPFRSTYFACLSMAAEMSTGVLAMAQLYKRNPPVS
                     MLVVHTEAAYFKKATGLTRFVCTDGPAIKSAVEQAITTGEAITIKARSLGSNSAGDPV
                     AEFHITWSFKLKKS"
     gene            complement(1606..>3071)
                     /locus_tag="VKQ52_22270"
     CDS             complement(1606..>3071)
                     /locus_tag="VKQ52_22270"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HLZ90002.1"
                     /translation="VILIGGYFLLGRSGCSNLIGGGGAGGGGNNAVQQLFTGGMLDPK
                     QFEKANIYESLDSTKDPLPEAANLQKYAPSVGNQGHQGSCVAWSSAYAARTIEEAARS
                     GADPNSLRFSPSFLYNQIGLENCDGSYIERAMEFMTKSGSVPYSQFPYNDKDCSKQPD
                     QQLLDEARQYKMRGFNRLTPGDRNNVIDLHAIKENISQGAPVVIGMQVGGTYMQDMMG
                     KDLWEPTAEDRSMLGFGGHAQCVVGYDDRKYGGAFLIMNSWGPEWGNNGFTWVRYPDF
                     KRFVREAYGIEPMARTGAAANEPFACEIGLVQVHYEGTKTIPGDYIPLQVKNSNVFET
                     VAPVKMGTRFKMEVKNTTECYVYVFGKETDGTSYTLFPYPDPNNPQKTKYSPFCGITG
                     YRLFPKGKSMMPDSIGTRDQMAVVVSKQPLNWYALNNTLSQNPGQDYGSRLNAALAAQ
                     LVRNVQFKSSAKGNMQFTVSGDKDNSVVVTIVEISKH"
BASE COUNT          562 a          687 c         1097 g          725 t
ORIGIN      
        1 gtccccgggc agatattggc gctgacgggg gaaagggtct ggacggaagg cgatccgatg
       61 acatacaagg tgtcgacacc gacgctcgtg ttggagcagc cggaactggg gtcggaaatg
      121 ataaaccggt agagatatcc gttcatggag gcactgaggt tccaggtaag gtcgtaggtg
      181 tttgtgccgg tgtagacagc gccatcactg atattactcc aggtggaacc agcgtcggtg
      241 ctcaactgcc attggtagtt ggagctgggg tcgacggcaa gggccgagcc ggtgccctcg
      301 gaagagcatt gcacgatgga ctggcctgtg acctgcgcca ccggggtggc gtttaccgtc
      361 agcacgccgg cggtggaaga gctggatccc aggctgttgg tgacggtaca gcggtagctg
      421 tacccgttga aggaagtcgg gatgcttgtg atggtgaggc tggttgtcgt ggcgccgctg
      481 tagttggcgt tgttggcgat ggccgtccag gtgctgccgt tgttgatact gacctgccat
      541 tggtaggtaa gggtcggggt acctgtagcc gatacggaga aagtggcgga ggtgccggag
      601 caaaccgttt gacttgtcgg cgagggggaa gaaacggtgg gctgcgagcg ggccgccatg
      661 gttgcgctga tgaacaggat gcaacacaag atcatgtttt tcataaataa gggtcgggtt
      721 atgataagat aatggaatca gtcgggctaa aattattgcg atttttcggg taatagaata
      781 gcggcccgag cggcttgcgg cgctttggag aggaaccggc aggggcgcct aaaaaaggtt
      841 ggggaattgt cctggcgcca catagaccat atgcgcaatc gcatatgtat aacaggtaga
      901 tttatcggcg actacctccg gcgcggagcg tgcacggcgg gagtagcgtt ccgtcgctaa
      961 gaggaagtgc ctaagacggc ccggggagtg cggtcggtat tagttgacga cggcctagat
     1021 gcttttaatt agccggtaag caaaacccat tatacctgta tcgtagtcct ttaattttag
     1081 cttttcttta gtttgaacga ccaggtgatg tggaattctg ctacggggtc gccggcactg
     1141 ttggagccga gggaccgcgc ttttatcgtg atggcttcgc ccgtggttat ggcctgttcg
     1201 acggcggatt ttatcgccgg tccgtcggta cagacgaacc gggtgaggcc ggtggccttt
     1261 ttgaagtaag ccgcttccgt gtggacgacg agcatggaga cgggtgggtt tcgtttatag
     1321 agttgcgcca tggcgaggac gccggtgctc atttcggcgg ccatgctgag gcaggcgaaa
     1381 taggtggagc ggaaggggtt gcgggtgaac caacggtaag ggacggtaac ggtgcactga
     1441 tcttcgtcca cctgcggaat gagcaggccg gagaagaagg cgctggggag tttggtcagg
     1501 aggtagagcc gggctttcag cgggttgcgg atgagacgta agaaggcggt cgggttttgg
     1561 ccggaggcag ggcgcgaggg atctggtatc ggttgagcca tggcgctaat gtttacttat
     1621 ttcgacgata gtgacgacga cgctgttgtc cttgtcgccg ctgacggtga attgcatgtt
     1681 cccttttgca gaggatttga actggacgtt gcgcaccagc tgggcggcga gagcggcgtt
     1741 gaggcggctg ccatagtcct ggccggggtt ttggctgagg gtgttgttca gcgcgtacca
     1801 gttgagcggt tgtttgctga cgacgacggc catctggtct cttgtgccga tgctatcggg
     1861 catcatgctc ttgccttttg ggaagaggcg gtagccggtg ataccgcaga agggggagta
     1921 ttttgttttt tgcggattat tgggatccgg ataggggaag agggtatagc ttgttccgtc
     1981 ggtctctttg ccgaagacat agacatagca ttcggtggta ttctttacct ccattttgaa
     2041 gcgggtgccc atttttacgg gggcgacggt ctcgaacacg ttactgttct ttacttgcag
     2101 ggggatatag tcgccgggga tggtttttgt gccttcgtag tggacctgga cgaggccgat
     2161 ctcgcaggcg aagggttcgt ttgcggcggc gccggtgcgg gccatgggtt cgatgccgta
     2221 ggcttcgcgg acaaagcgtt tgaagtcggg gtagcggacc caggtgaagc cgttgttacc
     2281 ccactcgggg ccccagctgt tcatgatgag gaaggcgcca ccatatttgc gatcgtcgta
     2341 ccctacgacg cattgcgcgt gacctccgaa gccgagcatg ctgcggtctt cggcggtggg
     2401 ttcccagagg tctttgccca tcatgtcctg catataggtg ccaccgacct gcatgccgat
     2461 gacgacgggg gcgccctggg agatattctc ttttatagcg tggaggtcga tgacattgtt
     2521 gcggtcgccg ggggtgaggc ggttgaagcc gcgcatttta tattgccggg cttcatcgag
     2581 gagttgctgg tcgggttgtt tggagcagtc cttatcgttg tacgggaatt ggctataggg
     2641 gacggagccg gactttgtca tgaattccat ggcgcgttcg atatagctac cgtcgcagtt
     2701 ctcgaggccg atctggttat agaggaagga ggggctgaag cggaggctgt ttgggtcggc
     2761 gccgctgcgg gcggcttctt cgatcgttcg ggccgcgtag gcactggacc aggcgacgca
     2821 ggagccctgg tggccctggt tgccgacgct gggggcatat ttctggaggt tggcggcttc
     2881 ggggaggggg tcctttgtgg agtcgaggga ttcgtagata ttggcctttt cgaattgttt
     2941 tgggtcgagc atgccgccgg tgaagagttg ctggacggcg ttgtttccgc cgccgccggc
     3001 gccaccgccg ccgatgagat tgctgcagcc gctgcggccg aggaggaagt agccgccgat
     3061 gaggatgacg a
//
LOCUS       DATGFG010001174         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1561154, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001174 DATGFG010000000
VERSION     DATGFG010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561154"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..2216)
                     /locus_tag="VKQ52_22275"
     CDS             complement(201..2216)
                     /locus_tag="VKQ52_22275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HLZ90003.1"
                     /translation="MRLLLVVLLLLFCCFARAQKEGQALIDSLVSALPHAANDTIRVK
                     TYNKATIYYVDVNTDSALKYADLGMGLAGRMNWTRGIAAFHTAYANVFTNRGQLDSAL
                     NRHRRALDLFLQIKDSVNIATANNNLGTIAKAKGDFVAAARYFLNTLEIGKAIKDNYL
                     IGLGSENLGLVYEYQEDYPKGLDYARQSVSAYTLNENHDHLPGPLCVIGSIFLRLNQG
                     DSAIYYLRKALVLARTNGNKIQEATLLNYVAEYYSGRQDYSNAIKNGLEAKKIWDVTG
                     PAAEDAINNTGILGYYYLQLAKRGPADNREKLLLLASTYLEEAVQKSRTKSIRTAQSE
                     FQVSLAEADALAGNYKGAYFNYKSYQEIRDSVYSQESKNKIAAAMSKLELDKKSAEIA
                     LNQATISNQHREELFLTVGLFLVVIIGGLLYRQSMMRKKTNTTLLVLNNELDEANKVK
                     AKFFGILSHDLRSPIANLINFLQLQKRRPGLLSEAQIADRETKITDSASQLLETMEAM
                     LLWSKGQMEHFKPALSVVSVESLFTFLRKNFADTEQVVLYFSPAGNLDLVTDEHYLQT
                     ILYNLTANAMKALRQKTDGIITWKAWRERDGLFLSISDNGPGASDEQLKALYDEAAPS
                     GARTGLGLHIIRDLAKAIGCVVTYTAGNSRGAEFILCMPASRLPATA"
     gene            complement(2226..2933)
                     /locus_tag="VKQ52_22280"
     CDS             complement(2226..2933)
                     /locus_tag="VKQ52_22280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLZ90004.1"
                     /translation="MTPKISCLVVDDDEVDLHTIISFLEDYPYLEVTGRCDSAADGLA
                     AAQKKAPDALFLDIDMPGMNGMELRQQLLHIPACIFITSYPDYALESFEMAALDFLVK
                     PFSPERFAKTAVRLQDYFTIRRKAELLNHTLGADTIFIKDGTKQVKLQLHEIIYFEAL
                     NNYTSIVTTSRKYAVLSTLGNLLKEEAFGNFIRIHRSFAVQKHFIEKVGPGQVLAGNI
                     PLPVGRNYKDSLNSLKP"
     gene            3086..>3439
                     /locus_tag="VKQ52_22285"
     CDS             3086..>3439
                     /locus_tag="VKQ52_22285"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HLZ90005.1"
                     /translation="MKTPRLFPHWPLVMLLLLSAACKKTPPIATPIPAITSITPASGS
                     AGTSVTITGTDFSLVAADNTVKFNGTAASITSATAGTLVVVAPSGGSTGHVTVATPGG
                     TANGPVFTYVALPPPP"
BASE COUNT          777 a          912 c          888 g          862 t
ORIGIN      
        1 ggttttgttt ttccgggagc ggggaaagct tgctttccaa agcggacggg aaaacaaaac
       61 cgtcccgacg aagtcggggc gaggcttttt gggtaataag gaggggggaa ggatcagcga
      121 agctaatcct gtcttcccga caatggtcta aagctataag ctatcaaagc ctccgcaaac
      181 gcggaggttt ttgttgttag ctatgcagta gccgggaggc ggctggcagg catacataaa
      241 ataaattccg cccccctgct gttccccgcg gtataggtca ccacgcagcc gatggccttt
      301 gccagatccc tgataatatg caggccgaga ccggtccgcg ctccgctggg ggcagcttcg
      361 tcgtacaggg ctttcaattg ctcgtcgctg gcgccggggc cattgtccga aatggagagg
      421 aatagcccgt cccgttcccg ccaggctttc cacgtgataa tcccatccgt cttctgccgc
      481 agtgccttca tggcattggc cgtgagattg tataggatgg tctgcaggta gtgctcgtcg
      541 gtcacgaggt ccaggttgcc tgccggagaa aaatagagaa cgacctgctc cgtatccgcg
      601 aaatttttcc gcaggaaagt aaaaaggctt tccaccgaaa cgacggaaag cgccggttta
      661 aaatgttcca tctgaccctt gctccaaagc agcatggcct ccatcgtctc cagcagctgg
      721 ctggcagaat cggtgatctt tgtttcccgg tcggcaattt gcgcttcgct caggagtccg
      781 ggtcgcctct tttgcaactg gaggaaattg atgaggttgg cgatcgggct gcgcaggtca
      841 tggctgagga tgccgaagaa ttttgccttc accttattgg cttcgtcgag ctcattgttc
      901 agcaccagta aagtagtatt ggtcttttta cgcatcatgc tttgccggta cagcagtcct
      961 ccgatgatga ccaccaggaa taatcccacc gtcaggaata actcttcccg gtgctggttg
     1021 cttatcgtcg cctgattcaa cgctatttcg gcactcttct tatccagctc cagcttgctc
     1081 atggcggcgg cgatcttgtt cttactttcc tgagaataaa cggagtccct gatctcctgg
     1141 taactcttat aattgaagta agcgccctta taattccctg ccagcgcatc ggcttccgcc
     1201 agggagacct ggaattccga ttgggcggtc ctgatgcttt ttgtcctgct cttttggaca
     1261 gcttcctcca ggtaggtgga ggccagcagg agtagtttct cccggttatc agccgggccc
     1321 cttttggcaa gctgtaaata gtagtagcca aggatacccg tattgttgat ggcatcctca
     1381 gctgcaggtc ccgtgacatc ccatattttt ttagcttcca ggccattttt gatagcgttt
     1441 gaataatcct gccggccgga ataatattcc gccacgtagt ttaatagggt agcttcctgg
     1501 atcttgttgc cgttggtcct tgccaggacc agggccttgc gtaaataata gatggccgag
     1561 tcaccctggt tcagtcgaag gaaaatactc ccgataacgc acagtggccc ggggagatga
     1621 tcgtggtttt cattcagggt ataggcggaa acggactgcc tggcatagtc cagccctttt
     1681 ggataatctt cctggtattc atatacaagg cccaggttct cggagccaag gccgataaga
     1741 taattgtctt ttatcgcctt gcctatttcc agggtattca ggaagtaccg ggcagctgcg
     1801 acaaaatcgc ccttggcctt ggcaatcgtc cccaggttat tattggcggt ggcaatgtta
     1861 acgctatcct tgatctgtaa gaaaaggtcc agtgctctgc gatgcctgtt cagagcagaa
     1921 tccagctgtc ctctgttcgt aaaaacattg gcataggcgg tatggaaagc ggcaatgccc
     1981 ctggtccaat tcatccggcc cgccaagccc atgcccagat ccgcatattt caaagcggag
     2041 tcggtattga cgtccacgta atagatcgtg gccttattat aggtctttac cctgatcgtg
     2101 tcatttgccg catgaggcaa tgcggacact aaagaatcga tcaatgcctg tccttctttc
     2161 tgcgcccgcg caaaacagca gaaaagaaga agtagaacaa cgagtagtag ccgcatggta
     2221 agggatcagg gttttaagga gttaagcgaa tctttataat tccttcccac cggcaacggg
     2281 atatttcccg ccagcacctg gcccgggccg accttttcaa tgaagtgctt ttgcacggca
     2341 aagctccgat ggatgcggat gaaattaccg aatgcctctt ccttcaggag attgccaagc
     2401 gtggacagca ccgcatattt ccggctggtc gtcacgatgc tggtatagtt gttcaatgcc
     2461 tcgaagtaaa tgatctcatg gagctgcaac ttcacctgct tcgtcccgtc ttttataaag
     2521 atggtatcgg cgcccagcgt gtggttcagt aattccgcct tccgacggat ggtaaaatag
     2581 tcctgcagcc ggaccgccgt ttttgcaaac cgctccggcg aaaagggttt taccaggaaa
     2641 tccagtgcgg ccatttcaaa gctttccaac gcgtaatcgg gataggaagt aataaaaata
     2701 caggcgggga tatgtagcaa ttgctgtctt agctccatcc cattcatgcc gggcatgtcg
     2761 atatccagga agagggcgtc aggcgccttt ttttgtgcgg ccgccagccc atcggcagca
     2821 gaatcacacc tgcccgttac ttctaaataa ggatagtctt ccaggaaaga aatgatcgta
     2881 tgcaggtcca cctcatcgtc atccactacc aggcaggaaa ttttaggagt catcaggaga
     2941 agaattaggg ttgaggggtt ccttacaaat ataaataata atgcaaatat gtcgttgacc
     3001 aatttaaatt gtcgttgacc cattggctct tttcccggga attctttgaa ctaggtttgt
     3061 gtggtaacac aaaaacaagg tagaaatgaa aacaccccga ctatttccgc actggcctct
     3121 cgtaatgctg ttattgcttt cggcggcctg caaaaagact ccgccaattg ccacgccgat
     3181 ccccgccatc acctccatca ctcctgcctc cgggagcgcc ggcacctccg ttaccataac
     3241 gggcacggac tttagcctgg tcgccgcgga caatacggtc aaattcaatg gcaccgccgc
     3301 cagtattacc agcgctaccg ccggcaccct ggtagtggtg gcgccttccg gcggcagcac
     3361 gggacatgtg acggtcgcca cgccaggggg taccgctaat gggcccgttt tcacctatgt
     3421 ggccctgccg cccccaccc
//
LOCUS       DATGFG010001175         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1674795, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001175 DATGFG010000000
VERSION     DATGFG010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674795"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..2002
                     /locus_tag="VKQ52_22290"
     CDS             116..2002
                     /locus_tag="VKQ52_22290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90006.1"
                     /translation="MKCIITTGCLIAMLFTARAQLAPGAEIPASLNHLSYDGNGRLTV
                     KAGGQTYTDAARTDEYKLPGIMGVLTGTETGILLDLNRPGYNGTLAYGPLDETAEYPA
                     IAFLPRDVRIRDGKALLEMKKTFTRATDYFHLADAGKGILGYRIIDSVGRIIYEGRVA
                     FEGKGPYQVVPTITQGPMVNTVEPTSFVLAYETQVPLKTAVAIDGRTFSDDQETTRHE
                     LVITGLKPSTEYQYTITYGARAESHKMVTAPAEGSRQPFVFAFASANRATTGGGERDF
                     GGTNYQHTRAVMAAARMHKAVFLQCTGDFTTGANSSVGGHMMEYANFKRSLEPFWSRI
                     PVYVGCGDHETNKTTLRSEDPANRGYSVELFPYAEVSGEATFARAFVNPVNGPQSEDG
                     AAYDPNPNQSDFPTYKENVYYYTYGNIAMIVLNTEYWESKAPMITGGCPEGYIMDQQM
                     KWLKETMQHMENNPNIDHIFVNVHGAVFPNGDHLADAMYWNGDNTSRAVVAGVPLPKG
                     AIERRDEIIDVCVNKSKKFLAFLSGDEHNFSFLEVTPETPIYKEGYTGPKIRVSRPFY
                     NINNGGAGAAPYAMLKSPWSGSFKYFTPPPSVALITVKGKNVVLSAVRVETLEPICQD
                     IELR"
     gene            complement(2020..>2684)
                     /locus_tag="VKQ52_22295"
     CDS             complement(2020..>2684)
                     /locus_tag="VKQ52_22295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLZ90007.1"
                     /translation="KEYLILKEFTENASHEMQTPLAIIRSKLDLAIQDQHLSEVQSVT
                     LRSAYASVKKLTSLNRSLLLIAKISNNQYSNSTVINIKDKLYDKIIQFQELWKDKLTV
                     TYNLDDAGITANPDLVDILLNNLFSNASKHNIQQGEVMIELTGTMLEIKNTGRPAPLD
                     ETRMFQRFYKVSLEDENNGLGLSIVKQICDLSGISSSYSFSGNIHAFTFKWTRPDRQV
                     DA"
BASE COUNT          655 a          687 c          700 g          642 t
ORIGIN      
        1 acacaaaccg actatactcc cggattctat ttattgcaac ttgatcgggc tcaccggtgt
       61 taccagagca acgacagcgg aatgagcaat taatgactaa accaactatc taacgatgaa
      121 atgtatcatt acaacgggat gtttaatagc tatgctcttc acggcccggg cgcaacttgc
      181 accgggtgct gaaatacccg cttcgcttaa ccacctttca tatgatggca acggccggtt
      241 gactgtaaaa gccgggggcc aaacctatac cgatgcggcc aggacggacg agtataaatt
      301 acccgggata atgggcgtgt tgacgggcac ggaaaccggc atcttacttg atctcaaccg
      361 tccgggatat aatgggaccc ttgcctatgg ccccctcgac gagacggcgg aatatcccgc
      421 catcgccttt ttgccgcgcg acgtcaggat cagggacgga aaagccctgc tcgaaatgaa
      481 aaagaccttt accagggcca ccgactactt ccatctggca gatgcgggaa aagggatact
      541 cggttatcgc attatcgaca gtgtgggccg cattatttat gaaggccgtg tagcgttcga
      601 agggaaggga ccttaccagg tggtccccac cattacccag ggcccgatgg taaataccgt
      661 ggaacccacc agtttcgtac tggcttatga aacacaggtt ccgcttaaaa cagctgtcgc
      721 gatcgatggc cggaccttct ccgatgacca ggaaactacc cggcatgagc tggtgatcac
      781 cggcctaaaa ccctccaccg aataccagta tacgatcacc tacggcgcgc gagccgaaag
      841 ccataaaatg gtgacggcgc cggcagaagg cagccgtcag ccttttgttt ttgccttcgc
      901 cagtgccaac cgggctacca ccggaggcgg tgaaagggat ttcggcggca caaattacca
      961 gcatacccgt gccgtcatgg cggccgcgag gatgcacaag gccgtcttcc tccagtgcac
     1021 cggcgacttt acgaccggag ctaattccag tgtgggcggt catatgatgg aatacgccaa
     1081 ctttaagcgc tcgttggagc cgttctggtc caggatacct gtatacgtcg gctgtggcga
     1141 tcatgagacc aataagacta ccctgcgtag cgaagatccg gccaatagag gttactccgt
     1201 cgaattgttc ccatatgccg aagtttccgg cgaggcaact tttgccaggg cattcgtcaa
     1261 tcctgtcaac ggtccccaaa gcgaggatgg agcagcttat gatcccaatc ccaaccaatc
     1321 ggattttcct acctataagg aaaatgtata ctactacacc tatggcaata tcgccatgat
     1381 cgtcctgaat acggagtatt gggaaagcaa ggcgccgatg ataacaggtg gctgcccgga
     1441 aggttatatc atggatcagc agatgaaatg gctaaaggag acgatgcagc atatggagaa
     1501 caatcccaac atcgaccata tttttgtgaa tgtccacggg gcggttttcc ccaatggaga
     1561 ccacctggcc gatgcgatgt actggaatgg ggacaatact tcgagagcag tcgttgcagg
     1621 tgttccgttg ccgaaaggcg ccatagaaag acgggatgag atcatcgacg tctgcgtcaa
     1681 caaaagcaag aagtttcttg ccttcttatc cggtgatgaa cataatttct ccttcctgga
     1741 ggttacgccc gagacgccca tctataagga gggctataca ggcccgaaga tccgggtcag
     1801 ccgtcccttc tacaatatca acaatggcgg cgccggcgct gccccctatg ccatgctgaa
     1861 aagtccgtgg tcggggtcgt tcaaatattt tacccctccc ccgagcgtcg cgttgatcac
     1921 cgtaaaagga aagaacgtcg tgctgtctgc ggtgcgggtg gagaccctgg agccgatctg
     1981 ccaggacatt gagttgcgct aaagtgctta tttcgggtac taggcatcga cctgccggtc
     2041 aggcctggtc catttgaaag tgaacgcgtg tatgttcccg ctaaacgaat acgaagagct
     2101 gatccccgac agatcgcaga tctgttttac aatggataac ccaagtccgt tgttctcatc
     2161 ctccagggat actttgtaga atcgttggaa catccgggtt tcgtccaatg gggcaggcct
     2221 gccggtattt tttatctcca acatagtgcc ggtgagctcg atcattactt cgccctgctg
     2281 gatattgtgc ttgctggcgt tgctgaacaa attgttcagc aggatatcta ccaggtctgg
     2341 gttggccgtt atcccggcat cgtccaggtt gtaggtaacc gttaatttgt ctttccataa
     2401 ctcctgaaac tggatgatct tatcatacag tttgtccttt atattgataa ccgtgctgtt
     2461 gctgtattga ttattgctga tctttgcaat cagtagcagc gatcggttaa ggcttgtcaa
     2521 ctttttaacc gaggcatagg cggaccgtaa ggttacgctc tgtacttcgg aaaggtgttg
     2581 atcctgtatg gccaggtcca gtttggaccg gataatggca agcggcgttt gcatttcatg
     2641 ggatgcattt tcggtaaatt ccttaaggat aaggtactct ttat
//
LOCUS       DATGFG010001176         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_188231, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001176 DATGFG010000000
VERSION     DATGFG010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188231"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /locus_tag="VKQ52_22300"
     CDS             complement(<1..1792)
                     /locus_tag="VKQ52_22300"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLZ90008.1"
                     /translation="MRNINLQHRLLFFAFFLLFLQVRLSAQVTTSSVGGTVTDNGGKP
                     LSGATVEISFPDAGIKRHIVTGSGGSFFVPNLRVGGPYTITVSYTGFNPRTENNIMLE
                     LGQTSTVDFKMELAAANLSAVTVTGRSRVFNENRTGASVNISSQQIQRLPTISRSADD
                     YTRLTPSASPTYNGVSFAGRNGQYNNYSLDGAVFNNPFGLDAPTPGGQASAQPISLDA
                     IDQIQVNIAPYDVTQAGFTGAGINTVTKSGTNKTFGTIYGFFRNQALTGHKVEGNSFN
                     VPSLHDFQGGASLGGAIKKNKLFYFVNFETQQRSDANSAYVANDGTNAGQITTSRVLK
                     SDLDAVSALLKNNYGYVTGPYQNYTQNTVSYKWIAKLDWVINDRNTLTFTYNGLSAYQ
                     DKPAHPSAINRRGPDAITLQYQNSGYRMYNHLENFGLELKTNFNSTVSNNLRVINTIF
                     HDFRNPNSTPFPVINITKNNVPYIIAGEEPFSIHNILNQNALQLTDNVTVALDNHTLT
                     FGGSFESFRFENSFNLTGYGFDVFGSVDLSTFMQGGYPAAGDVTYAKNAAAADKWTWS
                     YFTVGQAALYAQDAWKISPTFKLTYGLRVDK"
     gene            1902..>2353
                     /locus_tag="VKQ52_22305"
     CDS             1902..>2353
                     /locus_tag="VKQ52_22305"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLZ90009.1"
                     /translation="MIKFILNNKLIQSDDPPGTLLLDCIRYREHLTGTKIGCREGDCG
                     ACTVLIGEWKGNKVVYRSATSCLTAIGNVHHQHVVTIEGLNGDKLNFLQQAICDEGGT
                     QCGFCTPGFIVSMAGFCLSDKAPTHAAAIAAVDGNICRCTGYKSIERAL"
BASE COUNT          548 a          673 c          584 g          548 t
ORIGIN      
        1 gcttgtctac ccgcaaccca taggtcagct taaacgtcgg ggagatcttc caggcatcct
       61 gtgcatacaa agcagcctga cccacggtga aatacgacca ggtccacttg tccgccgccg
      121 ccgcattctt cgcataggtg acatcaccgg cggccggata tccaccctgc ataaaagtgg
      181 acaggtccac cgacccaaat acatcgaacc cataacccgt caggttaaag ctgttctcaa
      241 accggaacga ctcgaaactt ccaccaaagg tcagcgtatg gttgtcaagc gccacggtga
      301 cattgtccgt cagctgcagc gcattctggt tcaaaatatt gtgaatgctg aaaggctctt
      361 ccccggcaat gatatagggc acattgttct tcgtgatatt gatcaccggg aagggcgtcg
      421 aattaggatt ccggaagtcg tggaaaatgg tattgatcac ccgcaaatta ttcgacaccg
      481 tactattgaa gttggtcttc aactccaacc cgaaattctc gagatggtta tacatccggt
      541 aacccgaatt ctgatattgc agcgtgatcg cgtccggacc ccgccggttg atcgcactcg
      601 ggtgcgccgg cttgtcctgg taggcgctca gcccgttata ggtaaaggtc agcgtgttcc
      661 ggtcattgat cacccagtcc aatttggcga tccatttata actgaccgta ttctgcgtat
      721 agttctggta cgggcccgtt acatacccat aattattctt caacagcgca ctcacggcat
      781 ccagatcgct cttcaacacc cgcgacgtcg tgatctgacc cgcattcgtt ccgtcattgg
      841 ccacatacgc gctgttggca tcgctccgtt gctgcgtttc gaaattcacg aaatagaaga
      901 gtttattctt tttgatcgcg ccgccaagcg atgccccgcc ctggaaatca tgcagcgacg
      961 gtacgttgaa ggaattgcct tccaccttat gccccgtcaa cgcctggttg cggaaaaaac
     1021 catagatggt gccaaaggtc ttgttcgtcc cgctcttggt aacggtattg atacccgcac
     1081 cggtaaagcc cgcctgcgtc acatcatatg gcgcgatgtt cacctgtatc tggtcaatcg
     1141 catccagcga gatcggctgt gcactcgcct ggccgccggg tgtcggcgca tccagcccaa
     1201 acgggttgtt gaagacggcg ccgtcaagtg agtagttatt gtattgtcca ttccgaccgg
     1261 caaaggacac cccgttatag gtaggggagg ccgaaggcgt cagccgcgta taatcatcgg
     1321 cactccggga gatcgtcggc aaccgctgga tctgctgcga actgatattc accgaagccc
     1381 cggtgcggtt ttcgttgaac accctgctgc gaccagtaac cgttacggcg ctcaggttgg
     1441 ccgccgccag ctccatcttg aaatccaccg tactggtctg acccagttca agcatgatat
     1501 tgttctccgt cctgggattg aagccggtat agctcaccgt tatcgtatat ggaccgccaa
     1561 cccgcaggtt gggcacaaag aaactgccac cactaccggt aacgatatgc cgcttgatac
     1621 ctgcatcggg aaagctgatc tccaccgtgg cccccgaaag tggcttcccg ccattatcag
     1681 taaccgtacc acccaccgag gaggtcgtca cctgggcact gagccgcacc tgtaaaaaaa
     1741 gaaggaagaa agcaaagaat aggagtctgt gctgaaggtt gatgtttctc attcagaata
     1801 gttttggttt gtatatgtag tgctctactc aatttaaccc aaaaatgcca gagttgttcg
     1861 ttttgcacgc aatattttgt aaattgtata tatggatttg catgataaaa ttcatcctaa
     1921 ataataaact catccagtcg gatgatcccc ctggaacgct cttactcgat tgtatccgtt
     1981 accgcgaaca cctcacgggc acgaagatcg gatgtcgcga aggggattgt ggcgcctgta
     2041 cggtcctcat cggggagtgg aaagggaata aggtcgtcta ccggtcggcc acctcttgcc
     2101 tgacggccat cggcaatgtc catcaccaac acgtagtcac catcgaaggc ctcaacggcg
     2161 acaaactcaa tttcctccaa caggccatct gcgacgaagg cggtacccag tgcggcttct
     2221 gtaccccggg ctttatcgtc tccatggccg gtttctgcct cagcgacaag gcgcccactc
     2281 acgcggcagc catcgcagcc gtcgacggta acatctgccg gtgcaccggc tacaaatcca
     2341 tcgagagggc cct
//
LOCUS       DATGFG010001177         5993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1561474, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001177 DATGFG010000000
VERSION     DATGFG010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561474"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /gene="recF"
                     /locus_tag="VKQ52_22310"
     CDS             complement(<1..729)
                     /gene="recF"
                     /locus_tag="VKQ52_22310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HLZ90010.1"
                     /translation="MLRIKQISLVGFRNYTQATFRFDKRIVGICGNNGVGKTNLLDAL
                     YYLCFTRSYFARGDQANVLHGAAGFRLEGHFDNAGQETKVVCILRENGKKEFLTDGQP
                     YEKFSRHIGRFPCVIIAPDDIQIITAGSEERRRFLDALLSQLDPVYLQNLIDYNKVLQ
                     QRNGFLKSLTEGRLKDKQLLEVYDEQLIATGTAVFQLRRDFLVQLLPMAGQFYTAIAG
                     AEEPVSLRYDSQLQHTPFPQLLRQG"
     gene            786..2342
                     /locus_tag="VKQ52_22315"
     CDS             786..2342
                     /locus_tag="VKQ52_22315"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLZ90011.1"
                     /translation="MNKLFGLLLTAGLLAPVLLPAQQEPIDTAMVKRIRQEGLGNSQI
                     PVIAHHLTDEAGPRLTNSPGFHRASDWIVGTLKSWGLSGAQAEPWGEFGYGWSAEKTS
                     LSMRTPYYSPLIAYATPWSGSTNGPVSAPVYVVEKMDSAWIAGHLGEMKGKVLVIQPQ
                     DSLLAPDFKPDATRFTDSALANIGDTYMISREMLRMYMPFFLKNMQMDKMLAGSGALA
                     VLHASEHRDGTVIVQTFGGWRKKDQPALPDLQVAKEDLLRLARLARGGTSVTVELQSD
                     TRLYDKDLTGHNVVAEIPGTDPTLKSEVVMLGGHLDSWSAGTGATDNGAGCIVALEAI
                     RILKDLGVKPRRTIRIALWDGEEQGLFGSFHYVKNHFGDPIDMKLKSEQAKISAYYNL
                     DNGSGKIRGIFAQGNDQAAAIFRQWLAPFEDLGATKVTLHNTGSTDHLSFDAVGIPGF
                     QFIQDELDYETRTHHTNEDNYDHLRMADLRQAATILASFVYFTAMRQQMIPRKPLPKP
                     EKFIFDDLFP"
     gene            2467..3462
                     /gene="pdhA"
                     /locus_tag="VKQ52_22320"
     CDS             2467..3462
                     /gene="pdhA"
                     /locus_tag="VKQ52_22320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HLZ90012.1"
                     /translation="MATKFSKETYLYWYELMQLIRQFELMAEEKYKMEGKIRGFFHAY
                     VGQEAIAAGCMTATKPDDPFITAYRDHGLALSKGVSANACMAELYGKATGAAKGKGGS
                     MHFFGVDVKFFGGHGIVGAQIGTGAGLAFAERYKGTDNVVLTFFGDGAARQGMLHETF
                     NLAMLWKLPVIFICENNNYAMGTSVERTSNVVDIYKLADAYEMPADSIDGMSPEAVHE
                     AVARAVKRARQGDGPTLLEIKTYRYKGHSISDPQKYRTKEEVDEYKQRDPIQTVLQTI
                     LTKKFATQEEIDAINKRVSDTVTESVKFAEQSPWPDDSEVLKDVYIDANYPFIVD"
     gene            3499..4179
                     /locus_tag="VKQ52_22325"
     CDS             3499..4179
                     /locus_tag="VKQ52_22325"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ90013.1"
                     /translation="MSEIKHPAHLEEEKNPVAELQNAWEKYGKAASYVLLAIVVIVGG
                     YFGYRDLISEPKEKQATEAMFRAEEYYRADSARLALNGDNINAGFLKVMSKYSGTKAA
                     NLAAFYAGSCYLKLGDFNNAVKYLKDFSTPVKQLQERAYGLLGDAYSEQGNKQEAADQ
                     YKKAGTYFEKDELFSPEYLFRSGYLYENMGKTQDAIAMYKLIKDKYPTSQRGADIDKY
                     LARLGVIQ"
     gene            4187..4684
                     /gene="ribH"
                     /locus_tag="VKQ52_22330"
     CDS             4187..4684
                     /gene="ribH"
                     /locus_tag="VKQ52_22330"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HLZ90014.1"
                     /translation="MAEVSNSNLLSTVAGIPDIGDACVVIVRTEWNAAIVGALEEGCK
                     AVLDKAGVAYRVITVPGAFEIPFGVRAYWDIHKYRDDRPQAFVALGCVLRGDTPHFEY
                     VCRAVTDGVLQLNLTLPIPTIFGILTVDNQQQADERIGGKHGHKGEEAAITALKMIAV
                     KKTFL"
     gene            4681..5397
                     /locus_tag="VKQ52_22335"
     CDS             4681..5397
                     /locus_tag="VKQ52_22335"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLZ90015.1"
                     /translation="MTVQLFIPCFVDQLYPQTAFNMVKVLEKAGVEVTYNTEQTCCGQ
                     PAFNAGFRDEAREVCTKFLKDFGGHDYVVAPSASCVGFVRNYYSALFNNSSLHNEVRD
                     IQKRIFEFSSFLVNVLGVEDVGAMLYGKATYHDSCAGLRECRIKEEPRKLLSRVKGLE
                     LLEMNDVETCCGFGGSFAVKFESISIAMADQKVNNALQTGADYIISTDVSCLMHLDGY
                     IRHKGHPIRTMHIADVLANF"
     gene            5642..>5993
                     /locus_tag="VKQ52_22340"
     CDS             5642..>5993
                     /locus_tag="VKQ52_22340"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HLZ90016.1"
                     /translation="MAYWLVKSEPSVYPWEQLEKDKETCWSGVRNYAARLHLRGMKKG
                     EEVLYYHSNEGMEIVAIAKVAREAYQDPTTDDERWVAVDLKPFKRLKKPVSLDQIKKD
                     KRLAGMALVRIGRLS"
BASE COUNT         1399 a         1544 c         1801 g         1249 t
ORIGIN      
        1 gccctgccgg agcaattgcg ggaagggcgt atgctggagc tggctgtcgt atcggaggga
       61 taccggttct tccgcgcccg cgatggcggt gtagaattgg ccggccatcg ggagcagctg
      121 tacgaggaag tcgcgccgga gctggaagac ggctgtcccg gtggcaatca actgctcgtc
      181 atagacttca agcaactgtt tgtctttcag ccggccttcg gtgagggatt tcaggaaacc
      241 gttacgctgc tgtaaaacct tgttgtagtc gatcaggttt tggagataga ccgggtccag
      301 ttgggagagc agggcgtcga ggaagcggcg gcgttcttcg ctgcccgcgg tgatgatctg
      361 gatatcgtcg ggggcgatga tcacgcaggg gaagcggccg atatggcggg agaacttctc
      421 atagggctga ccgtcagtaa ggaactcctt tttcccgttc tcgcgcagga tgcagacgac
      481 ctttgtctcc tggccggcgt tgtcgaaatg tccttcgagg cgaaagccgg cggcgccatg
      541 gaggacgttg gcctgatcgc cgcgggcgaa atagcttcgt gtgaagcaca aatagtacag
      601 tgcgtccagc aggttggtct tgccgacgcc gttgttgcca cagatgccga cgatcctttt
      661 atcgaagcgg aaagtagcct gtgtatagtt cctgaagccg actagcgata tctgttttat
      721 cctgagcaat aggtgaaatt cggctgtaaa atattacctt tcctgcaaaa ctaaacaagg
      781 ctgctatgaa taaacttttt ggattgcttc tcaccgccgg gcttttggcg ccggtgctgt
      841 taccggcgca acaggaaccg atcgatacgg caatggttaa acgaattcgc caggaagggc
      901 tgggcaacag ccagataccc gtcatcgcgc atcatcttac cgacgaggcg gggccgcggc
      961 ttaccaacag cccggggttt caccgggctt cggactggat cgtcgggacc ctgaaaagct
     1021 gggggctcag cggggcgcag gccgagccct ggggagagtt tggctatgga tggtcggccg
     1081 agaagacctc gctttcgatg cgtaccccgt actacagtcc gctgatcgcc tatgccactc
     1141 cctggagcgg cagtacgaac ggaccggtct ccgctcccgt ctatgtcgtg gagaagatgg
     1201 actcggcgtg gatcgccggc cacctggggg agatgaaggg caaggtcctc gtcatccagc
     1261 cgcaggacag cctgctggcg ccggatttca agcctgacgc cacgcggttc accgacagcg
     1321 cgctggccaa tatcggtgac acgtatatga tcagcaggga gatgctcagg atgtatatgc
     1381 ccttcttcct caaaaatatg caaatggaca agatgctggc cggcagcggc gccctggccg
     1441 tcctgcatgc ctccgagcac cgggatggaa cggtgatcgt acagaccttt ggcggatggc
     1501 ggaaaaagga tcagccggcc ctgcccgatc tccaggtggc caaggaggac ctgctccggc
     1561 tggcgcggct ggcgcgcggc ggtacgagcg tcacggtcga gttacagagc gatacccggt
     1621 tgtatgacaa ggatctcacc gggcataatg tcgtcgccga gatccccgga accgatccca
     1681 cgctgaagtc ggaagtggtc atgctgggtg gtcacctgga ttcctggtcg gcgggaacgg
     1741 gcgccacgga caatggcgcg gggtgtatcg tggcgctcga ggcgatccgt atcctgaaag
     1801 acctgggggt aaagcccagg cggacgatcc gcatcgcgct ctgggacggg gaagagcagg
     1861 ggttgttcgg gtctttccac tatgtgaaga atcattttgg cgacccgatc gatatgaagc
     1921 tgaagtccga gcaggcaaag atctccgctt actacaacct ggacaacggc tccgggaaga
     1981 tcaggggcat cttcgcgcag ggtaacgatc aggcggcggc gatcttcagg caatggctgg
     2041 cgcctttcga agaccttggg gccaccaaag tgacgctgca caataccggg tcgaccgatc
     2101 acctgagttt cgatgcggtg ggcatccccg gcttccagtt catccaggac gaactcgatt
     2161 acgagacgag gacccatcat accaacgaag acaactatga tcacctgcgg atggccgatc
     2221 tccggcaggc ggccaccatc ctggccagtt tcgtgtattt cacggccatg cggcagcaaa
     2281 tgatcccgcg gaagccgctg cccaagcccg aaaagttcat ttttgatgat cttttcccgt
     2341 aaaaggcgtt gagcggcagg gtcaaatgtg ggaaaaattg cattttaccc cgtatatttg
     2401 cgcctccgac ggcggacccg gcctccgggt cgtccgagcg gcgcaaatcc aaaatatttc
     2461 aaaaacgtgg ctaccaaatt ctcaaaagag acctacctgt attggtatga attaatgcag
     2521 ctcatccggc aatttgagct gatggcggaa gaaaaatata aaatggaggg taagatccgc
     2581 ggctttttcc atgcctatgt cgggcaggaa gcgatcgcgg cggggtgtat gacggctacc
     2641 aagcccgatg atccttttat cacggcctac cgggaccatg ggctggccct gtccaagggc
     2701 gtcagcgcca atgcctgtat ggccgaactg tatggtaagg cgacgggggc ggccaagggg
     2761 aaggggggta gcatgcactt tttcggggta gatgtaaaat tctttggcgg ccacgggatc
     2821 gtcggtgcgc agatcggcac cggagccggg ctggcatttg ccgagcgata caagggcacg
     2881 gacaatgtgg tgctgacctt ctttggcgac ggcgccgcgc ggcaggggat gctccacgag
     2941 acctttaacc tggcgatgtt gtggaagctg cccgtcatct ttatctgcga gaacaacaac
     3001 tatgcgatgg gtaccagcgt agagcgcacc tcgaatgtcg tcgatatcta taaattggcg
     3061 gatgcatacg agatgcctgc cgattccatc gatgggatga gtcccgaagc cgttcatgaa
     3121 gccgttgccc gggccgtcaa gcgcgcccgc cagggggatg gcccgacgct gctggagatc
     3181 aagacctacc gctataaagg gcactccatc tccgaccccc agaaataccg gacgaaggaa
     3241 gaggtggacg aatacaaaca acgcgacccg atacaaaccg tgctgcagac catcctcacc
     3301 aagaaattcg ccacccagga agagatcgac gcgatcaaca agcgggtgag cgataccgtg
     3361 actgaatcgg tgaaattcgc agaacagtcg ccctggccgg atgattcgga agtactgaag
     3421 gacgtctata tcgacgcgaa ctatcctttt atcgtcgact aaatccattg ttaactcaaa
     3481 ttttcattta catacgatat gtcagagatc aaacaccctg ctcatttaga agaagagaag
     3541 aacccggttg ccgaactcca gaatgcctgg gaaaagtatg ggaaagcggc gtcctacgta
     3601 ctattggcga tcgtcgtcat cgtaggcggc tatttcggtt atcgcgacct gatcagtgag
     3661 cccaaggaaa agcaggccac cgaggccatg ttccgcgcgg aagagtacta ccgggcagat
     3721 tcggcccggc tggcgctcaa cggcgacaat atcaatgccg gtttcctgaa agtgatgtcc
     3781 aaatacagcg ggacaaaggc ggccaatctc gcggccttct atgccggcag ctgctatctg
     3841 aaactgggcg acttcaacaa cgccgtcaaa tacctgaagg atttctccac gccggtgaag
     3901 cagctccagg agcgggctta cgggttgctc ggggatgcct attccgaaca ggggaataag
     3961 caggaagcgg cggaccagta taagaaagcg ggcacctatt tcgagaagga cgagcttttc
     4021 tcccctgaat atcttttccg cagcggctac ctgtatgaga atatggggaa gacgcaggat
     4081 gcgatcgcca tgtataaact catcaaggat aaatatccta cttcccagcg cggggcggat
     4141 atcgacaaat acctggcaag attgggcgtg attcaataat accgttatgg ctgaagtgag
     4201 caacagcaat ttattatcca ccgtcgcagg catcccggat atcggggatg cctgtgtcgt
     4261 tattgtccgt accgagtgga atgccgctat cgtgggtgcg ttggaagagg gatgcaaggc
     4321 cgtgctggat aaggccgggg ttgcctaccg cgtgatcacc gtacccggcg cctttgagat
     4381 cccctttggg gtccgcgcgt actgggatat ccacaaatac cgggacgacc ggccgcaggc
     4441 ctttgtggcc ctgggttgtg tgctgcgggg agacacgccg catttcgaat atgtgtgccg
     4501 cgccgttacc gacggggtgc tacagctgaa cctgaccctg ccgatcccaa ccatttttgg
     4561 cattctgacg gtagacaacc agcaacaggc cgacgaacgc atcggcggca aacacggcca
     4621 taaaggcgag gaggccgcta tcacggcgtt gaagatgatc gccgtcaaaa agaccttttt
     4681 gtgacggtac aattgttcat accatgtttt gtggaccagc tttatccgca gaccgccttt
     4741 aatatggtga aggtcctgga aaaggccggc gtggaagtaa cctataatac cgagcagacc
     4801 tgttgcggcc agcctgcctt caacgcaggg ttccgggacg aggcgcgcga ggtctgcacg
     4861 aaattcctga aggactttgg cgggcatgac tacgtcgtgg cgccaagcgc cagttgtgtt
     4921 ggattcgtcc ggaattacta ttccgcctta ttcaataatt cttccctgca caacgaggta
     4981 cgtgacattc aaaaacgcat ctttgaattc tcttcttttc ttgtcaacgt actgggggtg
     5041 gaagatgtgg gcgccatgct gtatgggaag gccacctatc acgatagttg cgcggggctg
     5101 cgggaatgcc ggataaaaga agaaccgcgt aaactgctgt cgcgggtgaa ggggttggag
     5161 ctgctcgaga tgaatgatgt ggagacctgt tgcgggtttg gcgggagctt tgccgtgaag
     5221 ttcgagtcca tctccatcgc catggcggac cagaaagtaa acaacgccct gcagacgggc
     5281 gccgactata tcatttccac cgatgtttcc tgcctgatgc acctggacgg gtatatccgc
     5341 cataaggggc atccgatccg cacgatgcat attgcggatg tactggcgaa tttttaggta
     5401 gatcaataac caccgggcaa aaaaataaga ccacccaaat tacttatgta accggatggt
     5461 cttgaccctt aaaacaacct gtcgcaagcg ttaaataggg ttaaaaccaa gcctcactta
     5521 tacatacgag acccgataat ttttggattt aagaaagggc actttttttg aaaaaagcgt
     5581 ctcctgggtg ataatcagcc gtttacaaga ttaatttacc gaagaatgat tatttttggg
     5641 catggcatac tggcttgtaa aatcggaacc ctcggtatat ccctgggagc aactggaaaa
     5701 agacaaggaa acctgctgga gcggagtccg gaactatgcg gcccggctgc atctgcgggg
     5761 gatgaagaag ggggaagagg tattgtatta tcacagcaat gaaggaatgg agatcgtcgc
     5821 catagcaaag gtggcaaggg aagcctacca ggatcctacc accgatgacg agcggtgggt
     5881 ggcggtggac ctgaagccgt tcaagcgttt gaagaaaccc gttagtcttg accagatcaa
     5941 gaaggacaag cggctggccg ggatggcgct ggtccggatc ggccggttgt cgg
//
LOCUS       DATGFG010001178         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1265436, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001178 DATGFG010000000
VERSION     DATGFG010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265436"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VKQ52_22345"
     CDS             complement(<1..602)
                     /locus_tag="VKQ52_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90017.1"
                     /translation="MITHLPLGKKTGLFLLTIFLLLHTSTHAQIITTVAGGNIGDGKT
                     ATTIGLASADALVADANENIYFLDRGHNCIRRIDAATNIITTVAGGGNNTLSEGAPAL
                     SVDLFLSNIQDGIALDSHNHLYFLCFSEIVQLDLGTGLIHIVAGKINSTPSGDGGPIA
                     NATINFPLSLAVDNQDNLYFADYNSIRKVTLATGIVNTIAG"
     gene            complement(738..1853)
                     /locus_tag="VKQ52_22350"
     CDS             complement(738..1853)
                     /locus_tag="VKQ52_22350"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HLZ90018.1"
                     /translation="MRTQLLAALIAFAFFLANPQATHAQRPLDSVMPVRGFCIDLPRP
                     NGLDSFVRFIHEELAPRKVNTLFLLVDYHYQFKTHPELIDSFALSNTEVKKIVKACAQ
                     HNIRIIPQINLLGHQGWEEHPGKLLQAYPEFDETPWVKNPEYYVWPNADNLYCRSYCP
                     LHPKLHEVLFAVIDELCDAFESSAFHAGMDEVFYLAEAKCPRCGGQDPAVLFANEVRT
                     IHDHLAQKGRECWIWGDRLIDGKTTGIGMWEASYNNTWRAIDLIPKDVVICDWHYERP
                     DKTAPWFAMKGFRVVTCPWRNSGTGITQVEDMVSFRDGSTPEMKPRFYGIVETTWSPT
                     RFFLNGFYGIGQTGTPRPNTAWKCFRDVFLRIDSLGD"
     gene            complement(1888..>2366)
                     /locus_tag="VKQ52_22355"
     CDS             complement(1888..>2366)
                     /locus_tag="VKQ52_22355"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ90019.1"
                     /translation="QTLYKATTNGLPFEYTFLDDDYRRLYLAETRVATLSQYAAALAI
                     VISCLGLFGLAAYTAQKRQKEIGIRKVIGAGVDNIVLLLSRDFLQLTGLALLIDFPTA
                     AWLLTRWLHGFAYRVPLKADSFLLAGGVTVLITILSISLQCIKAALLNPIKALHSE"
BASE COUNT          452 a          528 c          842 g          544 t
ORIGIN      
        1 ccggcgatag tgttgacgat accggtggcc aaggttactt ttcgtatgct gttgtagtcc
       61 gcgaagtaaa ggttgtcctg gttgtcgacg gccagggata gggggaaatt gatcgttgcg
      121 ttggcgatgg ggccgccgtc gccggagggg gtgctgttga tcttgccggc gacgatatgg
      181 atcaggccgg tgccgaggtc cagctgtact atttcggaga agcagagaaa atagagatgg
      241 ttgtggctgt cgagggcgat accgtcctgg atattcgata aaaagaggtc gacggatagg
      301 gccggcgctc cttccgatag cgtgttattc cctccgccgg cgacggtggt gatgatgttg
      361 gtggcagcgt cgatcctcct gatgcaattg tggccgcggt cgagaaaata gatattctcg
      421 ttggcgtcgg cgacgagtgc gtctgcggat gcaaggccga tggttgtggc ggtcttcccg
      481 tcaccgatgt tgccaccggc aacggtggtg atgatctggg cgtgggttga tgtgtggaga
      541 aggaggaata tggttagcag gaaaagaccg gttttttttc ccaggggcag gtgtgtgatc
      601 atatgcaggt ttaggaatac acagtaaaga taggccatcg gggactgggg cgcaaaggcc
      661 ggcctattaa tttttggcga attgtacagg cgtgccgggg cagggattag tcgtgtagcc
      721 agcgaccata ggtgtgatta gtcgcccagc gagtcgatac gcagaaagac atcgcggaag
      781 catttccagg cggtgttggg ccgtggggta ccggtctggc cgatgccgta gaagccattg
      841 aggaagaagc gggtagggct ccaggtggtt tctacgatac catagaagcg gggtttcatc
      901 tcgggtgtgg agccgtcgcg gaagctgacc atatcctcga cctgggtgat gcccgttccg
      961 gagttgcgcc aggggcaggt gaccacccgg aagcctttca tggcgaacca gggggctgtt
     1021 ttgtcggggc gttcgtagtg ccagtcgcag atgaccacgt cttttgggat gaggtcgatg
     1081 gcgcgccagg tgttattata gcttgcttcc cacatgccga tgccggtggt tttgccgtcg
     1141 atgaggcggt cgccccagat ccagcattcg cggccttttt gtgcgaggtg gtcatggatg
     1201 gtccggacct cgtttgcgaa gaggaccgcg gggtcctggc cgccgcagcg cgggcatttg
     1261 gcttcggcca ggtaaaagac ctcgtccatt ccggcgtgaa aggcgctgct ttcgaaggcg
     1321 tcgcagagtt catcgatcac ggcgaagagg acctcgtgca gttttggatg gagggggcag
     1381 taactgcggc agtagaggtt gtcggcgttt ggccagacat agtattcggg gttctttacc
     1441 cagggtgtct cgtcgaattc cgggtaggcc tggagtagtt tcccgggatg ttcttcccat
     1501 ccctggtggc ccaggagatt gatctggggg atgatgcgga tgttgtgctg cgcgcaggct
     1561 tttacgatct tttttacttc cgtgttggag agtgcgaagc tgtcgatcag ttccggatgg
     1621 gttttgaact ggtaatgata gtccaccagc aggaacaagg tgttgacctt acgtggcgcc
     1681 agctcttcgt gtataaagcg aacgaaggaa tcgaggccgt tggggcgggg caggtcgata
     1741 cagaaaccgc ggaccggcat cacgctgtcc aatgggcgct gggcatgcgt tgcctgcgga
     1801 tttgccagga aaaaggcgaa ggcgataagg gcggcaagca gttgggttct cataccgcca
     1861 aggtacagat ttgttgttat tctgtcttta ttccgagtgt aatgccttta tcgggttcaa
     1921 taatgccgcc tttatgcact gcaggctgat ggacaggatc gtgatgagga ccgtcacgcc
     1981 gcccgcgagc aggaagctat cggcctttag cggtacgcgg taggcgaagc cgtgcagcca
     2041 gcgggtcagg agccaggcgg ccgtggggaa gtcgatgagt agggcaagtc ccgtgagttg
     2101 gaggaagtca cgggagagga ggaggacgat gttgtctacg ccggcgccga tgaccttgcg
     2161 gataccgatc tccttttgtc ttttctgggc ggtgtaggcg gcgaggccga agagaccgag
     2221 gcaggagatg acgatggcca gtgcggcggc atattgggag agggtagcta cgcgggtctc
     2281 cgccaggtat agccggcggt agtcatcgtc gaggaaggtg tattcgaagg ggaggccgtt
     2341 ggtggtcgcc ttgtagaggg tttgca
//
LOCUS       DATGFG010001179         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_644669, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001179 DATGFG010000000
VERSION     DATGFG010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644669"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VKQ52_22360"
     CDS             <1..1456
                     /locus_tag="VKQ52_22360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HLZ90020.1"
                     /translation="SYTVYKMDVTKMTREALKDITMGTKEKDRAKNMFVLGFLYWMYN
                     RNMETTVRFLKEKFGKKPEILESNIRALQAGYNFGDTTETFTTTYRVEKAKMSPGVYR
                     SIMGNQALAYGLIAASQKSGLQLFLGSYPITPASDILHELSRYKSFGVRTFQAEDEIA
                     AITSAIGAAYGGSLGVTTTSGPGMALKAEAMGLAVMMEIPLLICDIQRGGPSTGLPTK
                     TEQSDLLQAYYGRNGECPMPVISASTPSDCFEAIYEAVRIAVQHMTPVIFLSDGYIAN
                     GAEPWKFPKSADLPPIEVKFKTQLGESEEQFQPYLRDEKLVRPWAIPGTPGLEHRIGG
                     LEKQNITGNVSYDPENHQLMVKIREEKINRIADHIPPQHLDSGPESGDLLVIGWGSTF
                     GAIKSAVAELQHEGYKVSHAHLRHLRPFPANLGEMIRRFKQVLIPEINNGQLIKIIRD
                     QFLVDAKGYNKIMGIPITKSELVLKLRELLGGHN"
     gene            complement(1499..>2429)
                     /locus_tag="VKQ52_22365"
     CDS             complement(1499..>2429)
                     /locus_tag="VKQ52_22365"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HLZ90021.1"
                     /translation="LTIIVIMKPVYSLTKRRNYERLMGTLAGAFVGLLILYFIKDTTA
                     LFVLMILFMIGTYVFIRTNYLVCVTLMTPYVLLLFHLLYPIDFRSILSDRVIDTVIGS
                     AISFVASLLIIPSWEHERITDFMTGALDANIAYFKDVAGAFLGRPVTVHQYKLSRKNA
                     FVALANLSDALSRMLSEPRRKQKSITEMHQFVVSNHMLTSHIATLAYYMDPFAARYAD
                     AAYQPVVDDIVCRLERSVDVLEDRITAEPRPPAKEELMILNERAKSEREFKPIVDQFN
                     FIAKVTTDIGKVSPPLHTALAAPGQEVGINPLF"
BASE COUNT          605 a          781 c          618 g          425 t
ORIGIN      
        1 cagctatacc gtctacaaaa tggacgtgac aaaaatgacc cgggaggccc tgaaggatat
       61 caccatgggc acaaaggaga aagatcgcgc caagaatatg ttcgtcctgg gattcctgta
      121 ctggatgtac aaccgaaaca tggagacgac cgtccgcttc ctgaaagaga aattcggcaa
      181 gaaaccggag atcctcgaga gtaacatccg cgcactccag gccggctata actttggcga
      241 tacgaccgaa acctttacca cgacctaccg cgtcgagaag gcaaaaatga gcccgggcgt
      301 ttatcgcagc atcatgggta accaggccct ggcctatggc cttatcgccg cctcgcaaaa
      361 aagcggcctc cagctgttcc tcggctccta tccgatcacc cccgcgagcg atatcctgca
      421 cgaactaagc cggtacaagt ccttcggggt tcgcaccttc caggcggaag atgagatcgc
      481 cgccatcacc tcggccatcg gcgctgccta tggcggctcc ctcggcgtca cgaccacctc
      541 cggacccggt atggccctca aagccgaagc catgggcctc gccgtcatga tggagatccc
      601 cctgctgatc tgcgatatcc agcgcggcgg cccctccacg ggcctgccga caaaaacaga
      661 acaaagcgat ctcctccagg cctactacgg tcgcaacggc gaatgcccca tgcccgttat
      721 ttcggcctcc acacccagcg attgcttcga agccatatat gaagcggttc gcatcgccgt
      781 acaacatatg acccccgtca tcttcctcag cgacggctat atcgccaacg gcgcggaacc
      841 atggaaattc ccgaagagcg ccgacctccc ccccatcgag gtaaagttca aaacacaact
      901 cggtgaaagc gaggaacagt tccaacccta tctgcgcgat gagaaactcg tccgcccctg
      961 ggccataccc ggcacccccg gactggaaca ccgcatcggc ggcctcgaaa aacaaaacat
     1021 caccggcaac gtcagctatg acccggaaaa ccaccagctg atggtaaaga tccgcgaaga
     1081 aaagatcaac cgtatcgccg atcatatccc tccgcaacac ctcgacagcg gtcccgaatc
     1141 cggcgacctg ctcgtcatcg gctggggcag caccttcggc gccataaaaa gcgccgtggc
     1201 cgaactgcaa cacgagggct acaaggtatc ccatgcccat ctccgccacc tgcgcccctt
     1261 ccccgccaac ctcggcgaaa tgatccggcg cttcaaacag gtgctcattc cggagatcaa
     1321 caacgggcaa ctgataaaga tcatccgcga ccagttcctg gtagacgcaa aaggctacaa
     1381 caagatcatg ggcatcccca tcacaaagtc cgaactggtg ctgaaattac gcgaactgct
     1441 cggcggccat aactaaagcc ggcccaacgg accacggccc ttcgcgcggc agccctcgtc
     1501 aaaacaacgg attgatcccc acctcctgcc cgggggcggc gagcgccgta tgcagcgggg
     1561 gactcacctt cccgatgtcc gtcgtcacct ttgcgataaa attaaactgg tcgacgatcg
     1621 gcttgaactc cctttccgac ttggcccgct cgttcaggat catcagctcc tccttggcag
     1681 gcggtcgggg ctcggccgtg atcctgtcct ccaggacgtc caccgaccgc tccagccggc
     1741 aaacaatatc gtcgaccacg ggctggtagg ctgcatccgc ataccgcgcg gcaaaagggt
     1801 ccatatagta ggccagcgtc gcgatatggg aggtcagcat atgattggac acgacgaact
     1861 ggtgcatctc cgttatactc ttctgcttcc tgcggggctc ggagagcatc cgcgacaagg
     1921 catccgaaag attggccagc gcgacaaatg cgttcttgcg cgagagctta tactgatgca
     1981 cggtcaccgg cctccccagg aaagcgccgg caacatcctt gaaataggcg atgttggcgt
     2041 ccagcgcccc cgtcatgaaa tcggtgatcc gctcatgctc ccagctgggg atgatcagca
     2101 ggctcgcaac aaaggagatg gccgagccga tgaccgtgtc gatcacccgg tcggaaagaa
     2161 tactccggaa atcgatggga tataaaaggt ggaacaacaa caatacatag ggcgtcatca
     2221 gcgtcacgca aacaagataa ttcgtgcgta tgaatacata cgtgccgatc atgaacagga
     2281 tcatcagcac aaagagcgcc gtcgtatcct tgatgaaata caggatcagc aacccgacaa
     2341 aggcccccgc aagcgttccc atcagccgtt cgtaattccg ccgcttggta aggctgtaaa
     2401 cgggcttcat gatcacgatt atcgtcagc
//
LOCUS       DATGFG010001180         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_341227, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001180 DATGFG010000000
VERSION     DATGFG010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341227"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            579..971
                     /gene="tssD"
                     /locus_tag="VKQ52_22370"
     CDS             579..971
                     /gene="tssD"
                     /locus_tag="VKQ52_22370"
                     /inference="COORDINATES: protein motif:HMM:NF037603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:1901998 - toxin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HLZ90022.1"
                     /translation="MSFKAKLNVGGKDVNVLNCNYALKQETDATGRPSSITRGGKINL
                     TVESTGDTTFFEWMTNNFERKDGTITFVKRDSDATMKQLKFTEAYVVDYRENFDATGD
                     NPVTETFTLSAREISVGSGKHTNEWVKM"
     gene            1025..1414
                     /gene="tssD"
                     /locus_tag="VKQ52_22375"
     CDS             1025..1414
                     /gene="tssD"
                     /locus_tag="VKQ52_22375"
                     /inference="COORDINATES: protein motif:HMM:NF037603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:1901998 - toxin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HLZ90023.1"
                     /translation="MSFLAKLAIDGEEANILYCNFHFTQVTDTTGRPSSVPQGGTVNL
                     TVESTGKVDLFDWMISPTQKKSGTITFYRRDTMSKLKTLEFTDAHCVDYNEVYQHNGE
                     FPMQIGVVLSAKELKLNGSNFKNNWPE"
     gene            1474..3291
                     /locus_tag="VKQ52_22380"
     CDS             1474..3291
                     /locus_tag="VKQ52_22380"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HLZ90024.1"
                     /translation="MAQLVNTTISIAGNPITQFSSFQLTQGIFSHHTFRLVCPAEAID
                     GNTGNVFNRSKGMMGANLVAQINSVGMSGQLRFSGIVTQVETSRFNSYNGDVIITGYS
                     PTIILDNGPHCKSWEKKAVKNIAQDVLKHFPQNLLNPKVSPQYPETLSYYVQYKETAW
                     QFLFRLCSTYGEWFFYDGRSLVIGAPPGGTSTALTFGSNLSRFTMALQVRPAALQMMA
                     YDYMNHQVYTSTPSGIEGRAGLNDLGKTALQASQTVYAATPKIWNNHFLTNKKQLDDV
                     VNIQSAMQSSNHVRFNGASGHPGVAIGGEISVQGTNVFSQSDETYGDYTVISVNHYAD
                     GQGNYNNDFIAIPASIRVPPVSTPMAPPCEAQSALVTDNHDPKGLGRVRVKFYWMNGA
                     EKTPWIRVTSPHGGGGKGMFFIPEVGEEVIAGFEGDSAVKPYVIGTVYHGQANNSFGN
                     SGNDVKALQTRSGNKVIMNDAAGSVFVEDKDGNSVLIDGAGNITVKANKTVLIEATDE
                     ITFKTKKITMLAENEIYMESKQLNGQLSETATLYGKNHVTVQSDSLISLESQNKVEAM
                     ANTEVNLSSQSKIGVNSTHIEVNGDAATNIRGGMVNLNT"
     gene            3297..>3836
                     /locus_tag="VKQ52_22385"
     CDS             3297..>3836
                     /locus_tag="VKQ52_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90025.1"
                     /translation="MIHEHNPVMQRLGEMAGRWRREVRPGHRLVRWLLRPEDSRMYEG
                     FCRLEASPHGNLDSLFVFFYTPFTEASAFSCSVLSDWLREYDENGEQRAALAAAGVKG
                     DWEVDVFRRAVAERDYAACDVLLPAMLHGYRLWLGMPDAVIVLALLPKQMTAPAAFSS
                     WLSEWLLRDMADGVQLLVFD"
BASE COUNT          890 a          967 c         1126 g          853 t
ORIGIN      
        1 gcggttcgag caggacccct tgcagaagga ccgcatccat ctcgatatcc acctgaagcc
       61 gtatttcccc gcgaagacct tcctgatcaa actggagggg cagaaggggg aggatgcgga
      121 gaagacggaa tgggcctcga agtacgaaca ggaatgattt ttgcatgacc gttagtccag
      181 cgaaatgctg cgtgagtgcg ggatgaatgc gcgcagatta tttttgcgtg attgtggata
      241 aaatcagatt gacaattatc gagctaaaat ttttaagtgt taacattgta gcacttcttc
      301 actatccaac cccttgcaag accagaccct ttaatattct cgtaattatc cccaagacgt
      361 agttcccctt tcgttctgat tggcacagta gcctgcatga gtcgttttga tttacgcgcc
      421 gtacgcggtg tgcaggattg tttttcttct cattcaccat cgcaggggca tcgtctcctg
      481 cgttgaccgg ttttgaatcg tcacaaagcc ggaacgggat cgtatttttt attttctggt
      541 ctttatacca ttttttaatc ttttaaaatt catgtgttat gtcattcaag gcaaaactca
      601 acgtgggcgg caaggatgtc aacgtactca actgcaacta tgcattgaag caggaaacgg
      661 atgctaccgg ccggccttct tcgatcaccc gtggcggtaa gatcaactta acggtagaat
      721 ccaccgggga caccaccttc ttcgagtgga tgaccaacaa cttcgagcgg aaggacggga
      781 cgatcacctt tgtcaaacgc gattccgatg cgacgatgaa gcagttgaaa ttcacggaag
      841 cttatgtggt ggattaccgg gagaacttcg atgccacggg tgacaacccc gtgacggaga
      901 ccttcacgct cagcgcgagg gagatctcgg tgggcagcgg caagcacaca aacgaatggg
      961 ttaaaatgta agtcattcct tcgggggcgg ggtctgcccg ccccttcatt taaatcatcc
     1021 ttttatgtca ttcctggcca agctagcgat cgacggggaa gaggcgaata tcctgtactg
     1081 caacttccat tttacccagg tgaccgatac gacgggccgt ccttcctccg ttccgcaggg
     1141 tggcaccgta aacctgaccg tggagagcac cggcaaggtc gatcttttcg actggatgat
     1201 cagtcccacc cagaagaaga gcgggacgat cactttttac cgtcgggata cgatgagcaa
     1261 gctgaagacc ctggagttca cggatgcgca ttgcgtggat tacaacgagg tgtatcagca
     1321 taatggggaa tttcctatgc agataggggt agtcctcagt gcaaaagaat taaaactcaa
     1381 tggatcaaat tttaaaaaca attggccgga gtaacgggtg cgccttcccg gcatcttttt
     1441 ttgcggcaaa ccataaacga cacaaccttt tttatggccc agttagtgaa cacaaccatc
     1501 agcatcgccg gcaatcccat aacccagttc tcttcctttc aactgaccca ggggatcttc
     1561 tcgcatcata ccttccggct ggtatgtccc gcagaggcga tagacgggaa caccgggaat
     1621 gtattcaacc gcagcaaggg gatgatgggc gccaacctgg tcgcccagat caattcggtg
     1681 gggatgagcg gtcagttgcg attcagcggg atcgtcaccc aggtggagac ctcgcgtttc
     1741 aacagttata acggggatgt catcatcacc ggctatagtc ccaccatcat cctggacaac
     1801 ggcccccatt gcaagagctg ggagaagaag gccgtgaaga atatcgccca ggacgtgctg
     1861 aagcattttc cacaaaactt attgaacccc aaggtgtcgc cgcaataccc ggagaccctc
     1921 tcctattatg tccagtataa ggagacggcc tggcaatttc ttttccggct gtgttcaacc
     1981 tatggggaat ggttctttta tgacgggagg tcgcttgtta tcggtgcgcc cccgggcggc
     2041 acgagcactg ccctcacgtt tggcagcaac ctcagccgat ttaccatggc cctgcaggtg
     2101 cgcccggctg cattgcagat gatggcttat gactatatga accaccaggt gtataccagc
     2161 accccgagtg ggatcgaggg ccgggcgggg ctgaacgatc tcggcaagac ggccctgcag
     2221 gccagccaga cggtgtatgc ggcgacgccc aagatctgga acaatcattt ccttacgaat
     2281 aagaagcagc tggatgatgt cgtcaatatc cagtcggcca tgcaaagcag caatcatgtg
     2341 cgtttcaacg gcgccagcgg tcacccgggt gtcgccatcg gcggcgagat cagcgtacag
     2401 ggaacgaatg tctttagcca gagcgatgag acctatggcg actatacggt gatttccgtc
     2461 aaccattatg cggatggcca ggggaattac aacaacgatt ttatcgccat acccgccagt
     2521 atccgggtac cgcccgtgtc cacgccgatg gctcctccct gtgaggcgca gagtgcgctg
     2581 gtgacggata accatgatcc caagggattg ggacgtgtgc gggtgaagtt ctactggatg
     2641 aatggcgccg agaagacccc gtggatccgg gtcaccagtc cgcatggggg cggcggcaag
     2701 gggatgttct ttatcccgga ggtgggggaa gaagtgatcg ccgggttcga aggcgacagc
     2761 gccgtgaagc cctatgtgat cggtaccgtc taccatgggc aggccaataa cagctttggt
     2821 aattcgggca acgatgtaaa ggccctgcag acccgcagtg ggaacaaggt gatcatgaat
     2881 gatgcggcgg gcagtgtctt tgtcgaggac aaggacggca acagcgtgct gatcgacggg
     2941 gccgggaata tcaccgtgaa ggccaataag acggtgctga tcgaagccac ggacgagatc
     3001 acgttcaaga cgaagaagat cacgatgctg gccgagaacg agatctatat ggagtcgaag
     3061 cagctgaacg ggcagttgtc cgagacggcg accctttatg gcaagaacca tgtgacggtg
     3121 cagtcggatt cgctgatcag cctggagtcg cagaacaagg tggaggcgat ggccaacacc
     3181 gaagtgaacc tgagcagcca gagcaagatc ggggtcaact ccacgcatat agaggtgaac
     3241 ggcgatgccg ccaccaatat ccgcggcggg atggtcaatc ttaatacctg agaattatga
     3301 tacacgagca taaccccgtg atgcagcgtc ttggcgagat ggccgggcgc tggcggcggg
     3361 aggttcggcc gggacaccgg ctggtccggt ggctgttgcg gccggaagac agccggatgt
     3421 acgaagggtt ttgccggctc gaggcttctc cgcatgggaa cctggacagt ctttttgtat
     3481 tcttttatac gccttttacg gaagcttcgg cattcagctg ttcggtgctg agcgattggc
     3541 ttcgcgagta tgatgagaat ggcgagcagc gggcggcgct ggccgcggcg ggggtgaagg
     3601 gggactggga ggtagatgtt tttcgccgcg cggtcgccga acgggactat gcggcctgtg
     3661 atgtgctgtt acccgcgatg ttacatggct atcgtctttg gctggggatg cccgacgccg
     3721 ttatcgtgct ggccttgctg ccaaagcaga tgactgcccc ggcggccttt tcttcctggc
     3781 tgtcggagtg gttgttgcgg gatatggcgg acggtgtgca gttgctggtg ttcgat
//
LOCUS       DATGFG010001181         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1015796, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001181 DATGFG010000000
VERSION     DATGFG010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015796"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..929
                     /locus_tag="VKQ52_22390"
     CDS             27..929
                     /locus_tag="VKQ52_22390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLZ90026.1"
                     /translation="MLKDRPLISIIALNWNTTAITCDFLVSIREQNSYPNIEVIVVDN
                     ASNEDPTSAFLQVYPEAKVVRNPRNLGFSAGNNAGIRKAAGDYLFIVNNDTEFTPGLL
                     EGLLEVYREHPDAGIVCPKFHYFFDKGTIEYAGYYRVNIFTGRNSMIGCREKDHGQYD
                     EMKETHYAHGGAMMVPRRVIDEVGVMPEEFFLYYEEFDWSEQIKRHGFKVYYQPKSLI
                     YHKESMTTGKSSPLKTFYLTRNRILFMRRNTPPLAFSLFALYFVFFTIPKNTLSYLVR
                     GQKEHLTSFWKGILWQFNRTITFN"
     gene            937..2838
                     /gene="asnB"
                     /locus_tag="VKQ52_22395"
     CDS             937..2838
                     /gene="asnB"
                     /locus_tag="VKQ52_22395"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HLZ90027.1"
                     /translation="MCGITGFVDFKKTTGIDVLRKMSDALLHRGPDDGGCEVYEAPGA
                     AIGFGQRRLSILDLSPLGHQPMHFGDLIVNFNGEIYNFKEIRKELEAKGYTFNSWSDT
                     EVILKGYDCWGIDVVQKFIGMFAFVLYDKRKQQIILLRDRAGVKPLYYYWNEEVLLFG
                     SELKALYAHPAFEKKIDVNSVALFLQFSYIPAPHSIFHRTYKLLPGHFLTIDLASRRQ
                     QLTKYWDVFDHYNKPKLHLSDQEAIEQTDALLKNSYEYRMVADVPVGLFLSGGYDSSS
                     VAALLQTGRAEKLKTFTIGFNENEFNEAPEAKKIAEYLGTDHTEWYLTAKEAGSVLDR
                     LPEIYDEPFADNSTVPTVLVSVLARKQVKVALSADGGDEIFGGYNKFNQAERYTSQFP
                     PFVQSLISGTMSLINPEYIPFFNSKYNFSTRYEKMRLIWKSHDPVSALKYISQYITEK
                     EAESFIGRPFERYHTFFEDGKLLEHNDGVVNRLLAIDYKTFLVDNNLVKMDRATMSVS
                     LEGREPMLDHRLIEFLAQLPSSLKIRNGVNKWLLKEVVHRYIPKELMERPKRPFIAPL
                     MVWFREDLKEQLRYYLSEKCLSATGVFAHGPIVQLRDEYLAGKKVNYQKLWQVLMFQL
                     WYDRWIEKL"
     gene            2852..>3376
                     /locus_tag="VKQ52_22400"
     CDS             2852..>3376
                     /locus_tag="VKQ52_22400"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLZ90028.1"
                     /translation="MGKKIRVLECIRQGKIGGGESLLLNLVEYLDKDRFEPVVLSFTQ
                     GPMIERLQQMGVGTDVIYTEKPFDLTKWGRVKDLLKEKEVDLIHAHGTRAASNVLWAA
                     RSLGIPVVYTIHGWSFHQDQRTLLKRMRIAGETYITARTAVNISVSEANQASGKENIR
                     GFESVLVNYGIDQKK"
BASE COUNT          784 a          739 c         1009 g          844 t
ORIGIN      
        1 taccttgcaa aaaaagaaga tcaactatgt tgaaggatcg tcctttaata tccattattg
       61 cgttgaattg gaacactacc gcaataacgt gtgattttct tgtttccatc cgggagcaga
      121 acagctatcc caatatcgag gtgatcgttg tggacaatgc gtccaacgag gacccgacgt
      181 ctgcatttct ccaggtctat cccgaagcga aggtcgttcg caatccccgt aatcttgggt
      241 tctcggcggg taataacgca ggtatccgga aggctgccgg cgattatctt tttatcgtca
      301 acaacgatac ggagtttacg cccgggctgc tggaggggtt gctggaggtg taccgggagc
      361 atccggatgc gggtatcgtc tgtcccaaat tccattattt ttttgacaag ggaacgatcg
      421 aatatgccgg gtattaccgg gtgaatattt ttaccggtcg caacagcatg atcgggtgcc
      481 gggagaagga tcacggccag tatgatgaga tgaaggagac gcattatgcc catggcggcg
      541 ccatgatggt gcccaggcgg gttatcgatg aggtcggggt gatgccggag gagttctttc
      601 tttattatga agagttcgac tggtcggagc agatcaagcg gcatgggttc aaggtctatt
      661 atcagcccaa gtcgctgatc tatcataagg aatcgatgac gacgggcaag tcgagcccgt
      721 tgaagacttt ttacctgacg cgtaaccgta tcctgtttat gcgccggaat acgcccccgc
      781 ttgccttctc gttgtttgcc ctctattttg ttttttttac catacccaag aataccctga
      841 gctatctggt caggggccag aaggagcatc tgacctcttt ctggaaaggc attttatggc
      901 aattcaatcg taccattact tttaactgat ctatttatgt gtggaatcac cggctttgtg
      961 gattttaaaa agactaccgg catcgatgtg ctgcgtaaga tgtcggatgc cctgcttcac
     1021 cgtgggccgg atgacggcgg ttgtgaagtg tatgaggcgc cgggcgcggc gatcggtttt
     1081 ggccagcgcc ggctgtcgat attggatctg tctccattag ggcatcagcc gatgcatttt
     1141 ggcgatctta tcgtcaattt caacggggag atctacaatt tcaaggagat aaggaaggag
     1201 cttgaggcta agggatacac cttcaattcc tggtcggaca cggaagtgat cctgaaagga
     1261 tatgactgct ggggtatcga tgtggtgcag aagttcatcg gcatgtttgc ctttgtcctt
     1321 tatgacaagc ggaagcagca gatcatcctg ttgcgtgacc gggcgggggt aaagcctttg
     1381 tattattatt ggaacgagga ggtgctgtta tttggctccg agttgaaggc gttgtatgcc
     1441 cacccggctt ttgaaaagaa gatcgatgtg aacagcgtgg cgcttttcct gcagttcagc
     1501 tatatccctg cgccacatag tatcttccac cggacctaca agctgttgcc cgggcatttt
     1561 ctgacgatcg acctggcgag ccggcggcag caactgacga aatactggga tgttttcgat
     1621 cattataaca agcccaagct tcatttgtca gaccaggagg cgatcgagca gacggatgcc
     1681 ctgttgaaga actcgtatga gtaccggatg gtggccgatg tgccggtggg gctttttctc
     1741 agcggcggct atgacagcag cagtgtggct gcgttgttgc agacggggcg ggccgagaag
     1801 ttgaagactt ttaccatcgg gttcaacgag aatgagttca atgaggcgcc ggaggcgaag
     1861 aagattgccg aatatcttgg gacggatcat acggaatggt atctgacggc gaaggaagcg
     1921 ggaagtgtgc tggaccggct gccggagata tatgatgaac cttttgcgga caactccact
     1981 gttcccaccg tactggtgag tgtgcttgcg cgtaagcagg tgaaggtggc gcttagcgcg
     2041 gacggcgggg acgagatctt tggcggctat aacaagttca accaggcgga aaggtatacc
     2101 agccagttcc cgccttttgt ccagagtctt atcagcggga cgatgtcact gatcaacccg
     2161 gagtatatcc cgttcttcaa cagcaagtat aatttttcga cgcggtatga gaagatgcgg
     2221 ttgatctgga agagtcatga tcccgttagc gcgttgaaat atatcagcca gtacattacg
     2281 gagaaagagg ccgagtcttt tattggaagg ccgtttgaaa gatatcatac cttcttcgaa
     2341 gacggaaagc tgctggagca taatgacggt gtcgtgaacc ggctgctggc catcgactat
     2401 aagacctttc tcgtcgacaa taacctggtg aagatggacc gggcgactat gtcggtgagt
     2461 ctcgaggggc gtgagccgat gctggatcac cggctgatag aatttttggc gcagctgcct
     2521 tcttccttaa agatcaggaa tggggtgaac aagtggttgt tgaaggaggt ggtgcaccgg
     2581 tatattccaa aggagctgat ggagcggccc aagcggccct ttatcgcgcc gctcatggtc
     2641 tggttccggg aagatctcaa ggagcaattg cgttactatc tgagtgagaa atgtctttcg
     2701 gcgacgggcg tgtttgcgca cggccccatc gttcaattac gggacgaata cctggccggc
     2761 aagaaagtga attaccagaa actatggcag gtgctgatgt tccagctttg gtatgaccgc
     2821 tggatcgaaa aattatagtt gcatacatat tatgggaaag aaaatcaggg tcctggagtg
     2881 tatccggcag ggtaagatcg gcggcgggga gtctcttttg ctgaatcttg tggagtatct
     2941 tgacaaggac cgtttcgagc cggtggtgct gagctttacg cagggcccca tgatcgaacg
     3001 gttgcagcag atgggggtcg ggacagatgt tatttatacg gaaaagcctt tcgacctgac
     3061 caagtggggg cgggtgaagg acctgttgaa ggaaaaggag gtggatctta ttcatgcgca
     3121 tggtacgcgt gccgccagca atgtgctttg ggcggcgcgg tcacttggga tccctgttgt
     3181 ctatacgatc catggctggt cttttcacca ggatcagcgg accctgctga agcggatgcg
     3241 gatcgccggg gagacctaca taacggcaag gacggcggtg aacatatctg tgtccgaggc
     3301 aaaccaggct tcggggaagg aaaatatccg gggctttgaa tcggtgcttg tgaattatgg
     3361 catcgaccag aagaag
//
LOCUS       DATGFG010001182         4654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1675196, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001182 DATGFG010000000
VERSION     DATGFG010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675196"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VKQ52_22405"
     CDS             complement(<1..921)
                     /locus_tag="VKQ52_22405"
                     /inference="COORDINATES: protein motif:HMM:NF027499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4874 domain-containing protein"
                     /protein_id="HLZ90029.1"
                     /translation="MVKASFGFAFLTLFITTQIHAQRPPAATTAIHYKEDTTDFPNPE
                     RGFYIPIGTSAGRFKPLDTETLIAIFTPEHKHGNARYTFHSTLLMREYTLDTFKAQPL
                     SKAFLDLIDQDLSIIRKASLKVILRFAYIDKTSTGNCPDPYKICPPYGDAPKAIVLQH
                     ITQLTPLLQKNADIIAVLQEGFIGIWGENFYTDYFGDPGRNGPGRIMDSSWDDRKEVL
                     DALLTALPADRMIQVRTPQIKQKAIYGAGAGIGSPPLTAANAFTGEPISRIGFHNDCF
                     LSSPDDYGTYYDYGSSNQPQKEANIPLRQYL"
     gene            complement(914..1612)
                     /locus_tag="VKQ52_22410"
     CDS             complement(914..1612)
                     /locus_tag="VKQ52_22410"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLZ90030.1"
                     /translation="MKDTPTGRRTFIRTATAFTGAMAGLAILPSAAMSSPFSRRVPPP
                     GPRAAADKDINIIGPKEGYSPQIGTLVSMMAWLRPQVIGSVKALTVEQLDFLLDDKAN
                     TIGALLYHLAATDAFYHEHTFKGIEWGKWDKSIADKFNPAMELGDIGRKQIKGNNVDF
                     YLNLLQETRESTLAEFRKRDDKWLMTVDEKWYWGPTNNYCKWFHVCEHESHHLGQINL
                     LKSRLPGAKPANNG"
     gene            complement(1701..2708)
                     /locus_tag="VKQ52_22415"
     CDS             complement(1701..2708)
                     /locus_tag="VKQ52_22415"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HLZ90031.1"
                     /translation="MPKLIPTTAWNSLSLAIARGDANRVQEIIEDQQLDVNACVDSSS
                     WMPVLMEALLSYGFPSEEERLPLLRYLLEKGANPNIVCVRGYNCLHIAAQQERYINAL
                     DLFLDFNADVNIGDADGSNIVYWAVQGWLLRREGADRTTYLRVLEKILHLGADLDQQN
                     RFGMNTRRWLEVASADVQELVQGWEAGRPAIHGVTTVQPVFPTNLRYPELVREIWDQF
                     VPTTGAPANTVQGELLRAVENLRDEGRRQSNGNSHPNTRPQITAHHRKLNKKQAIFVR
                     DTLLGSGLFNKIESERIRTETQRLIKLSHPWPADEVYDDLVDKICVFYTRHPQPIPLA
                     T"
     gene            complement(2752..3303)
                     /locus_tag="VKQ52_22420"
     CDS             complement(2752..3303)
                     /locus_tag="VKQ52_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90032.1"
                     /translation="MRKDTTNTTVHIRSILRLKDELKKNRANGLRFNYKYYAGDTHGS
                     VPLISEYDGLRFIFDFFNPPYSSMLFDDKSGADAIISAVKAHYRLVSDKMGYTILPPE
                     EWVNSAGYFYLQGGKFDRSAAFFKMNTENYPKSGNTFDSLGDLYSTTGDHQKAVEAYT
                     KALTLYDNADTKKKLEEQKSKMH"
     gene            complement(3335..3991)
                     /locus_tag="VKQ52_22425"
     CDS             complement(3335..3991)
                     /locus_tag="VKQ52_22425"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HLZ90033.1"
                     /translation="MKLLLPLLCLLTLWAKAQSNGDIVIGKTDTVYSHILNEKRRILV
                     RLPHDGTSPFFRGQRYPVIYLLDGDSHFPSVMGLIQQLTEVNSNSLFPDMILVGIPNT
                     NRQRDLTPTLAKTSPYGDSNSVRGTGGGENFTAFIEKELIPHIDSLYPTAPYRILIGH
                     SLGGLMVINTLINHPNLFSAYLAIDPSMWWDDRKLLKQAGTVLARQKIPRQDPLSRYR
                     "
     gene            complement(4094..>4654)
                     /locus_tag="VKQ52_22430"
     CDS             complement(4094..>4654)
                     /locus_tag="VKQ52_22430"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLZ90034.1"
                     /translation="IQGLQTGYPVIHYRLNPAAGIADDLAKAEKVFKQYNPQYPFEYY
                     FVDEYYNNKFRAEQREGTLGTFFASLAIFISCLGLFGLATHMAENRRKEIGIRKVLGA
                     SVTGIATLISGDFIKLVLVAVVIATPIAWYAMSGWLQGFNYRIAITGWIFLVSGLLAV
                     TIALLTVGYQAVRAALANPAKSLRSE"
BASE COUNT          900 a         1071 c         1570 g         1113 t
ORIGIN      
        1 caggtattgc cggagcggga tgttggcttc tttttgcggc tggtttgaag agccatagtc
       61 gtagtaggtg ccatagtcat cggggctgga gaggaagcag tcgttatgaa agccgatgcg
      121 ggagatgggt tcacctgtga atgcgttggc ggcggtgagg ggtggggatc cgatcccggc
      181 cccggcgcca tagatggctt tttgtttgat ctgtggggtg cggacttgta tcatgcgatc
      241 ggcggggagg gccgtgagaa gggcgtcgag gacttccttc ctatcgtccc agctgctgtc
      301 catgatccgg ccggggccat tcctgccggg atccccaaaa tagtcggtat agaaattctc
      361 gccccagatg ccgatgaatc cctcttgtag tacggcgatg atgtcggcat ttttttggag
      421 gagcggcgtc agctgggtga tatgttgcag gacgatcgcc tttggggcat cgccgtatgg
      481 ggggcatatc ttgtacggat cggggcagtt gccggtgctg gtcttgtcga tataggcgaa
      541 gcggaggatc actttgagag aggctttgcg gatgatcgaa agatcctggt cgatgagatc
      601 gaggaaggcc ttgctcagcg gctgtgcttt gaaggtgtcg agggtatact cccgcatgag
      661 gagggtggag tggaaagtgt agcgcgcgtt gccgtgctta tgttcggggg tgaagatcgc
      721 gatgagcgtt tccgtgtcga ggggtttgaa acggccggcg cttgtgccaa tggggatata
      781 gaagccccgt tcggggttgg ggaagtcggt ggtgtcttcc ttgtagtgga tggcagtggt
      841 ggcggcgggt ggacgctggg cgtgtatttg cgtggttata aagagggtga ggaaggcaaa
      901 accaaaactc gccttaacca ttgttggcgg gttttgcgcc gggcagccgg cttttcaaca
      961 aattgatctg accgaggtga tgggattcgt gttcacagac atggaaccat ttgcagtagt
     1021 tgtttgtcgg tccccagtac catttctcgt cgacggtcat cagccatttg tcgtcgcgtt
     1081 tcctgaattc cgcaagcgtg ctttcgcggg tctcctgcag gaggttgaga taaaaatcga
     1141 cattattgcc ttttatctgt ttgcggccaa tatcgccgag ttccatcgcc gggttgaatt
     1201 tatctgctat cgatttgtcc catttccccc actcaatacc tttgaaggta tgttcgtgat
     1261 agaaggcgtc cgtggcggcc aggtgataga gcagggcgcc gatggtattg gctttgtcgt
     1321 cgagaaggaa gtcgagttgt tcgacggtga gcgctttgac ggagccgatc acctgtggcc
     1381 ggagccaggc catcatcgat acaagggtgc cgatctgtgg gctgtagcct tcttttggcc
     1441 cgataatgtt aatgtctttg tccgcggcag cgcgtgggcc gggcgggggt acgcggcggc
     1501 tgaaggggga ggacatggcg gctgaaggca ggatggcgag tccggccatg gcgccggtga
     1561 atgcggtggc tgttcttata aaggtgcgcc ggccggtggg tgtgtctttc atggtgtaat
     1621 gtttaatgat tgccagaaag atacggctat tttgggaaaa cgggcggcgc tggataggga
     1681 tttgagaaga agtcgcgggg tcaggtggcg agagggatag gttgagggtg acgggtatag
     1741 aagacgcaga tcttgtcgac gagatcgtcg tagacttcgt cggcgggcca ggggtgcgag
     1801 agttttatga ggcgctgggt ttcggtgcgg atgcgctcgc tttcgatctt gttgaagagg
     1861 ccggagccga ggagggtgtc gcgaacgaag atagcctgct tcttattcag tttgcggtga
     1921 tgcgcggtaa tttggggccg ggtattgggg tgggagttgc cgttggattg gcggcggccc
     1981 tcatcgcgga ggttttcgac ggcgcggagg agttccccct gcaccgtatt ggcgggcgcg
     2041 ccggtggtgg gcacgaactg gtcccagatc tccctcacca gttcgggata gcgtaggttg
     2101 gtggggaata cgggctgcac ggtggtgacg ccatggatcg cgggccggcc ggcctcccag
     2161 ccttgcacca gttcctggac atcggccgat gccacctcga gccagcggcg ggtattcatg
     2221 ccaaagcggt tttgctgatc gagatcggcg ccaaggtgga ggatcttctc cagcacgcgc
     2281 agataggtgg tgcgatcggc tccttcgcgg cgcagcagcc agccctgcac cgcccagtag
     2341 acgatgttgg agccatcggc atcgccgatg ttgacgtctg cattaaagtc gagaaagaga
     2401 tcgagggcgt tgatgtagcg ctcctgctgg gccgcaatgt gcaggcagtt atagccgcgg
     2461 acgcagacaa tattggggtt cgcgcctttt tccaacagat accgcagaag gggaagccgt
     2521 tcttcttcgg agggaaaacc gtaggatagc agcgcttcca tcaggacggg catccaggac
     2581 gagctgtcga cgcaggcgtt gacgtcgagt tgttggtctt caattatttc ctgtacccgg
     2641 ttggcgtctc cgcgtgcaat agcaagggag agcgaattcc acgcagtggt gggaatcagt
     2701 tttggcaaag caatcataat accaaaagat acaaaaaata gccgagtcct cctagtgcat
     2761 ttttgacttc tgctcttcca gcttcttttt tgtgtcggcg ttgtcgtaca gggtcagggc
     2821 ctttgtatag gcctcgacgg ctttttgatg gtcgccggta gtactgtaga ggtcgccaag
     2881 gctgtcgaag gtgttgccgc ttttcgggta gttttccgtg ttcattttga aaaaggcggc
     2941 cgaccggtcg aatttcccgc cctgcaggta gaaatagccc gccgaattga cccattcttc
     3001 gggtggcagg atggtatacc ccattttgtc ggagacgagg cggtagtgtg cctttacggc
     3061 gctgatgatg gcgtccgcgc cggacttgtc atcaaacagc atcgagctat agggcgggtt
     3121 gaaaaagtcg aaaatgaagc gaagtccgtc gtattcactg atgaggggca ccgaaccgtg
     3181 ggtatcaccc gcataatatt tatagttgaa gcgcaagccg ttggccctgt tctttttcag
     3241 ctcgtccttc agccggagga tgctgcggat gtggacggtg gtattggttg tgtcctttct
     3301 cacccttgcg gtatccattc ccgggggcat tgtgttagcg atagcgagat agagggtctt
     3361 gcctgggtat tttttgccgg gccagaacgg taccggcctg tttcaggagt ttccggtcat
     3421 cccaccacat gcttgggtcg atggccaggt aggcgctaaa gaggttgggg tggttgatca
     3481 gggtattgat gaccatgagg ccgccgaggg aatgaccgat caggatgcga tagggcgcgg
     3541 tgggatagag cgagtcgata tgcgggatga gttccttttc gatgaaggcg gtgaagttct
     3601 cgccaccgcc ggtgccgcgg acgctattgc tgtcgccata ggggcttgtc ttcgccaggg
     3661 tgggcgtcag atcgcgctgg cggttggtat tggggatgcc gacgaggatc atgtcgggga
     3721 agagggagtt gctattcacc tcggtcaatt gctggatcag tcccatgacg gaaggaaagt
     3781 gggagtcgcc gtcgagcagg tagatgacag gatagcgctg cccgcggaag aaggggctgg
     3841 ttccgtcgtg cggcaggcgg accaggatgc gccgcttctc gttgaggata tgcgaataga
     3901 cggtgtccgt tttgccgatg acgatgtctc cattggactg ggctttcgcc caaagggtaa
     3961 gtagacagag aaggggcaac aatagtttca tgaccttttt gtttgaacag taatatacat
     4021 aaacccgcta gccggccggg ggctttgtga tgaatgtatg caactgtggg acgaattacc
     4081 cgcctgctaa ggatcattca ctgcggaggc ttttagcggg attggcaagc gccgcccgca
     4141 ccgcctggta gccgaccgtc agtaacgcga tcgtcacggc cagcagcccg gagacgagga
     4201 atatccagcc ggtgatggcg atgcggtaat tgaacccctg cagccagcca gacatggcat
     4261 accacgcgat gggagtggcg atgaccacgg ccacgagcac cagttttata aaatccccgg
     4321 agatgagggt ggcgataccc gtcaccgatg ctcccagtac cttccggatg ccgatctcct
     4381 tgcgccggtt ctcggccata tgggtcgcga ggccgaagag cccgagacag gaaatgaata
     4441 tggcaagcga cgcgaagaag gtgccgagcg tgccttcccg ttgttcggcc ctgaacttgt
     4501 tgttataata ttcatcgacg aagtaatact cgaagggata ttggggatta tattgcttga
     4561 ataccttttc ggcctttgcg aggtcgtcgg caatgccggc cgcgggattg agccggtagt
     4621 gaataactgg gtagcctgtc tggagtccct gtat
//
LOCUS       DATGFG010001183         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_491822, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001183 DATGFG010000000
VERSION     DATGFG010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491822"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..2595
                     /locus_tag="VKQ52_22435"
     CDS             220..2595
                     /locus_tag="VKQ52_22435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLZ90035.1"
                     /translation="MLRNYFKTTFRGLWKNKTYGFLNIAGLAIGITSAAFIFLWIEDE
                     TTFNHNFAKRNLLYHIMQNEKSDRGVFTNGSTPGPLAAALKAEIPGVRNSGRLSWAMD
                     ELAVVGDKSIKQNGIYADPSVLSMYTLLFVRGDAASAFQQVQSVIISESMARRFFGEE
                     NPIGKTITMTANQGYSVDGLYTVTGVFKDLPANCYYHFQWVSPYEAWENANPWVKPWD
                     NAMAETIVELDPTASPVAIDKQLTHYLGTKLAGAKNALFLFSMNDWHLRANFENGVQT
                     GGTIRYVRLFSLIAIAILLIACINFMNLATARSDQRAKEVGVLKVMGAGRGRLIGKFI
                     SEALVLAFLAVGLAIGLLYLLLPAYNALVNKQLSIHLFQFPHWSALLAIAVASGLVAG
                     SYPAFYLSSFNPVFVLKGLKIKTSGNAVFIRKGLVIMQFTASVILIICTVIVYQQVEH
                     IRNRNLGYNMNKLVYMDLQGNMKKDFEVIKNKLLATGYVENAATSLHDALHVYSYGNG
                     FGWQGKDPNMKVPVHSNVVSPEYLSTMHMQLIDGRDFYPTPGVDSTNWIINESMAKLM
                     GKEGKVGAILTSGQYQGHVVGIIKDFVYNDIYGSGAPLVLFPGLYGATVMALRFKENA
                     PLSQALAATQSLITGENPGHPFEYKFADENFNALFSTETLIGKLAGVFATLAVFISCL
                     GLFGLAAYTAERRAKEIGIRKVLGASTQGLAGLLSREFLALVTLSCLIAFPLAWWAMR
                     GWLQGYEYRTTIHWWIFAVAGALALVVALVTVSFQAVRAALANPIKALRSE"
     gene            complement(2844..3176)
                     /locus_tag="VKQ52_22440"
     CDS             complement(2844..3176)
                     /locus_tag="VKQ52_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90036.1"
                     /translation="MKKLLFVLLCLGFSAAVFAQTDQTKKAEMKDLRSDVRAHKAATH
                     KVNHDLAHVRVSKAMQDHKAVHRLNKDENSDAKRLKEQGVSHPVTKAKRQVKVQEDNR
                     KDHTGPGR"
BASE COUNT          712 a         1179 c          764 g          650 t
ORIGIN      
        1 cacccccagg cagcatcctc cagctaaccc gcagctcctc aactgccggc cccggcaaca
       61 ccgcccgacc gcaaaccggc ctgccccctc aactgccggc cccggcaact gccggtcccg
      121 caaacacctc cagcctccga aaaataatcc cccgatcccg gtaaccaaaa cccatctccg
      181 aagtattcca aacaagccat tcaccgcaaa acccgccata tgctccgcaa ctacttcaaa
      241 acaaccttcc gcggcctctg gaaaaacaag acctacggct tcctcaacat cgccggcctg
      301 gccatcggca tcacctccgc cgccttcatc ttcctgtgga tcgaagatga aaccaccttc
      361 aaccacaatt tcgccaaaag gaatctgctc tatcacatca tgcagaacga aaaaagtgac
      421 cgcggcgtct tcaccaacgg ctccaccccc ggccccctgg ccgcggctct gaaggccgag
      481 atccccggcg tcaggaacag cggccgcctc agctgggcca tggacgagct cgccgtggta
      541 ggcgacaaat cgataaagca gaacggcatc tatgccgacc cctccgtcct ctccatgtac
      601 accctcctct ttgtccgggg cgacgcggct tccgcgttcc aacaggtgca gtccgtgatc
      661 atcagcgaat cgatggcccg gagattcttt ggcgaagaga accctatcgg caaaacgatc
      721 accatgaccg ccaaccaggg ctactcggtc gatggcctat acaccgtcac cggcgtcttc
      781 aaagacctcc ccgccaactg ctactatcat tttcaatggg tctcgcccta cgaagcctgg
      841 gagaacgcca acccctgggt aaaaccctgg gataacgcca tggccgagac catcgtagag
      901 ctggacccca ccgcttcccc ggtcgccatc gacaaacagc tcacccacta cctcggtaca
      961 aagctcgccg gcgcgaagaa cgccctcttt cttttcagca tgaacgactg gcacctccgc
     1021 gccaacttcg agaatggcgt gcaaaccggc ggcaccatcc gctatgtgcg cctgttctcg
     1081 ctgatcgcca tcgccatcct gctcatcgcc tgtatcaatt ttatgaacct cgccacggcc
     1141 cgctcggatc agcgcgccaa agaggtgggc gtactaaaag taatgggcgc cggccgcggc
     1201 cggttgatcg gaaaatttat cagcgaagcc ctcgtactcg ctttcctggc cgtcgggctg
     1261 gccatcggcc tgctgtacct cctcctgccg gcttataatg cgctggtcaa caaacagctc
     1321 tccatccatc tgttccaatt cccgcactgg tccgccctgc tggccattgc ggtagcatcg
     1381 ggcctcgtcg cgggcagtta ccccgccttc tacctctcct ccttcaaccc cgtcttcgtg
     1441 ctaaagggcc tgaaaataaa gaccagcggc aatgccgtct tcatcagaaa gggcttggtc
     1501 attatgcagt tcaccgcgtc ggtgatcctg atcatctgca ccgtcatcgt ctatcagcag
     1561 gtggaacaca tcaggaaccg caacctcggg tacaacatga acaagctcgt ctatatggac
     1621 cttcagggga atatgaagaa ggacttcgag gtcattaaaa acaagctcct ggccacgggc
     1681 tatgtggaaa atgccgctac cagcctgcac gacgccctgc atgtatatag ttatgggaat
     1741 gggtttggct ggcaggggaa ggaccccaac atgaaggtcc ccgtccactc caatgtcgtc
     1801 agcccggaat acctttccac gatgcatatg caactcatcg acggcaggga tttttatcca
     1861 acccccggtg tggacagcac gaattggatc atcaacgaaa gcatggcgaa actcatggga
     1921 aaagagggta aggtcggcgc catcctcacc agtggccaat atcagggcca cgtggtaggg
     1981 atcataaaag acttcgtgta taacgacatc tatggctccg gcgccccgct ggtcctcttt
     2041 cccggcctct atggcgccac tgtaatggcc ctgcggttca aagaaaacgc ccccctctca
     2101 caagcgctcg ccgccaccca atccctgatc accggcgaaa atcccggtca tccctttgag
     2161 tacaaatttg ccgatgagaa ttttaatgcc ctcttctcca ccgagaccct catcggcaag
     2221 ctggccggcg tctttgccac cctcgccgtg tttatctcct gcctcggctt attcggcctc
     2281 gccgcctata ccgccgagcg ccgcgcaaaa gagatcggca tccgcaaagt actgggcgcc
     2341 agtacccagg gtctcgccgg ccttctctcc cgcgaattcc tcgccctcgt caccctctcc
     2401 tgtcttatcg ccttccccct cgcctggtgg gccatgcggg gctggctgca agggtatgaa
     2461 taccggacga ccatccactg gtggatcttc gccgtcgccg gtgcgctggc cctcgtcgtc
     2521 gccctcgtca ccgtcagttt ccaggcggtg cgggcggctc tcgccaatcc gataaaggca
     2581 ttgagaagcg aatagggcaa gccgaaagcc gccacccgct aacgccggcc ccggcaacac
     2641 cgcccgaccg caaaccggcc tgtcccctca actgcccgcc cccgctaaca ccgccctgca
     2701 aaccgcccgg ccgcatcctc caccagcccg cggcccctcc caacatgccc aaccacccca
     2761 ccggcatgca cacccacaaa aacgccgggc agactcaaca tccacccggc gcaccttctt
     2821 tcgccaaaaa ctcccgaccc ctcctaccgg cccggaccgg tatggtcctt ccggttatcc
     2881 tcctgcacct ttacctgccg cttagccttc gtcacggggt gactcacacc ctgctccttc
     2941 agccttttgg catcggaatt ctcatcctta ttgagccggt gcaccgcctt atgatcctgc
     3001 atagccttgc tgacccggac atgcgccaga tcatgattca ctttgtgggt ggcagccttg
     3061 tgcgcccgca catcactgcg cagatccttc atctcggctt tctttgtctg gtcggtctgg
     3121 gcaaatacag cggcgctgaa acccaagcac agcaatacaa ataatagctt tttcatgttt
     3181 tccattttaa tgagcaaatc gcacatcgac cccggtcagc ttcagcccag gcaagcccgc
     3241 ctccggccaa actccatggc cccgtgcaca ctgattacaa tgaacatagt gctatttcca
     3301 tgcca
//
LOCUS       DATGFG010001184         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1271944, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001184 DATGFG010000000
VERSION     DATGFG010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271944"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..571)
                     /locus_tag="VKQ52_22445"
     CDS             complement(146..571)
                     /locus_tag="VKQ52_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90037.1"
                     /translation="MFTKWTILIFTAGVFVTGCNDRSSSTKDTEVLTGSPWKYEKVGF
                     DDEEGTFDALDPRIAGNERDNVIIFCKDGTGYSEFTHRSKAGNPDSLPFIWAFQNDST
                     IYFQDQYYRVRVLTQQRLELYADEKLGGTNTRYTIILKR"
     gene            749..1750
                     /locus_tag="VKQ52_22450"
     CDS             749..1750
                     /locus_tag="VKQ52_22450"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLZ90038.1"
                     /translation="MLYTAEDIRQLNNKIQYQAAFIDRLRDEVGRVIVGQHQMLDRLL
                     IGLLSNGHVLLEGVPGLAKTLAIKSLSQAIHAKFSRIQFTPDLLPADVIGTMIYNQQK
                     HEFVVRRGPIFANFVLADEINRAPAKVQSALLEAMQERQVTLGDTSYKLEEPFLVLAT
                     QNPLEQEGTYPLPEAQQDRFIMKVLVDYPTKQEEQLIIRQNVQGLQSTQVEQVVTMQE
                     IMQAKDLSRQIYMDEKVEHYILDIVFATRSPEKYKLEKLKPLIAYGGSPRASINLALA
                     AKANAFLNKRGYVIPEDVRSISKDVLRHRIGLTYEAEAESVNVENVIDEVLKVIPIP"
     gene            1769..2686
                     /locus_tag="VKQ52_22455"
     CDS             1769..2686
                     /locus_tag="VKQ52_22455"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLZ90039.1"
                     /translation="MLTTTEILKKVRELEIKSKKLTRHLFTGEYHSAFKGRGMSFREV
                     REYAAGDDIRFIDWNVSARFNHPFSKLFEEERELTVMLLVDVSASSVFGTTHARKKDI
                     ITEVCAVLAFSAVNNNDKVGAIFFSDKVEGYIPPKKGREHVLYIVRQLLTIEPKRKGT
                     NLNEAIRFLNRSARQKAIVFFLSDFLDGAGEQRLTTTRPEPGFEDALKVAGKKHDMIG
                     IKVYDRMDMQLPAIGMVEAEDSETGGRYWVDTNDYLVRTNYQQYFFNHTEQCKSIFQK
                     AGCDLLHLRTDEDYVKILQRFFVGRHRPG"
     gene            2690..>3378
                     /locus_tag="VKQ52_22460"
     CDS             2690..>3378
                     /locus_tag="VKQ52_22460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90040.1"
                     /translation="MRPIRSYTIVALLWLPAMHLHAQRKPAQAPPPAIVKTTIDKDSI
                     LIGQPIQLMLEATVPDNIPFAWPATDSLPHFEWLEKGKIDTTVRPGQRYYRQYFTITS
                     FDSGTWAIPRLTFLAGTKKALTDSVRITVGYSKIDPAKDFHDIKDIVEIPNPFARWIP
                     WIIAAVTVLSLVLVFWLIRKRKIIKQLVIRQLPRLSPFEEAMQQLDELAKQQLPVNGA
                     IKTYYSRLDEI"
BASE COUNT          913 a         1037 c          751 g          677 t
ORIGIN      
        1 gagaggaacg ttaggcggcg gatcagattt ttcatggttt taattacgag gttatgcaaa
       61 ggtcagacca aggtaagacg atttgagcag gattactata gtagttttac tataagtgtt
      121 tactcttgaa ggagatagcg atcgtttatc gtttcaggat gatcgtatag cgcgtgttcg
      181 tccctcccaa cttctcgtcc gcataaagct ccagccgctg ttgcgtcagc accctgaccc
      241 gataatactg atcctggaaa taaatggtgc tgtcattctg aaaagcccag ataaaaggaa
      301 gactgtcggg attgccggcc ttgctccgat gggtaaactc ggaatacccc gttccgtcct
      361 tgcaaaatat gatcacatta tcccgctcat tgcccgcaat acgtggatca agcgcatcga
      421 aagtcccctc ctcatcgtca aaacctacct tctcatactt ccacggactc cccgtcaata
      481 cctccgtgtc cttggtcgaa gaactccgat cattacaacc cgtgacaaaa acacccgcgg
      541 taaagatcag tatggtccat ttcgtaaaca tcaggcgcat cattttccat tagaaggtgc
      601 ggtattactc aagtttaaaa ctcgaaaccc ctgcaactta ttgttaacat atgtaataat
      661 ttccccatga attatccaca accggcgaat aagtgcatct cccgtacata tttcgtattt
      721 tcgggccccc aaagtaatat ttcatcatat gctttataca gcagaggata tcagacagtt
      781 gaataataag atccaatacc aggcagcctt tatcgatcgc ctgagggacg aagtaggaag
      841 ggtcatcgtc ggtcagcacc agatgctcga ccgcctcctc atcggcctgc tcagcaacgg
      901 ccacgtcctg ctggaaggcg tccccggcct cgccaaaaca ttggctatta agtctttgtc
      961 gcaagccatc cacgccaagt tcagccgcat ccagttcacg cccgacctgc tcccggccga
     1021 cgtcatcgga acgatgatct acaatcagca aaaacacgaa ttcgtcgtcc gcagaggacc
     1081 catattcgcc aatttcgtcc tcgctgacga gatcaaccgc gcccccgcta aagtacaaag
     1141 cgcgctgctc gaagcaatgc aggaaagaca ggtcaccctc ggggatacca gctataaact
     1201 ggaagaaccc ttcctcgtcc tggcgaccca aaaccccctc gaacaggaag gaacataccc
     1261 cctccccgaa gcgcagcagg atcgcttcat catgaaagtg ctcgtcgact atcccacaaa
     1321 acaggaagaa cagctgatca tccgccagaa cgtccagggc cttcagtcca cgcaggtgga
     1381 acaggtggtg accatgcagg agatcatgca ggcaaaggac ctgtcccgcc agatctacat
     1441 ggacgaaaag gtcgaacact atatcctcga tatcgtcttc gcaacccgct cacccgaaaa
     1501 atataagctg gaaaagctaa aaccgctcat cgcttacggc ggaagcccaa gggccagcat
     1561 caacctcgcc ctggccgcaa aagcaaacgc ttttctgaac aaacgcgggt atgtcatccc
     1621 cgaggacgta cgctccatct cgaaagacgt gctgcgccat cgcatcggcc tcacctacga
     1681 agccgaagcc gaaagcgtga acgtcgagaa cgtcatcgac gaagtgctga aagtcattcc
     1741 cataccataa tagcctatct tcgttcgcat gctcacaacc accgaaatat taaagaaggt
     1801 ccgggagctg gaaataaaaa gtaaaaagct cacccgccat cttttcaccg gggaatacca
     1861 cagcgccttt aaaggccgtg gtatgagctt ccgcgaggtc cgcgagtatg cggcgggcga
     1921 tgatatccgc ttcatcgact ggaacgtatc ggcccgcttc aaccatccct tcagcaaact
     1981 cttcgaggaa gaacgggaac tcaccgtcat gctgctcgtg gatgtcagcg catcctccgt
     2041 cttcgggacc acacacgccc ggaagaaaga catcatcacc gaggtctgcg ccgtactggc
     2101 cttctccgcc gtcaacaaca acgacaaggt gggcgccatt ttcttcagcg acaaggtcga
     2161 aggctatatt cctccaaaaa agggtaggga acacgtcctc tacatcgtcc ggcaactgct
     2221 gaccatcgag ccaaaacgaa aaggcaccaa cctcaacgaa gctatccgct tcctcaaccg
     2281 cagcgcccgg caaaaagcca tcgtcttctt cttaagcgac ttcctcgacg gcgccggcga
     2341 acaacgcctg acgacgaccc ggcccgaacc cggcttcgag gacgccctga aagtggccgg
     2401 caaaaaacac gacatgatcg ggataaaggt ctacgacagg atggatatgc aactgcccgc
     2461 catcggcatg gtcgaggcgg aagactccga aacaggcggc cgctattggg ttgacacaaa
     2521 cgactatctg gtgcgcacca actaccaaca atattttttt aaccacaccg aacagtgtaa
     2581 aagcattttt caaaaagccg gttgcgatct cctgcacctg cggacggacg aagactacgt
     2641 aaagatcctt cagcgattct tcgtcggccg ccaccggccc ggatagtaca tgagacccat
     2701 aagatcatat accattgtcg ctctcctgtg gctgccggcg atgcatctcc acgcacagcg
     2761 caaacccgca caggcgcccc cacccgcgat cgtaaaaaca acgatcgaca aggatagcat
     2821 cctcatcggc cagcccatcc aactgatgct cgaagccacc gtccccgata atatcccctt
     2881 tgcatggccc gccaccgaca gcctcccgca tttcgaatgg ctggaaaaag gaaagataga
     2941 taccaccgtc cgccccggcc agcgctacta ccggcaatat ttcaccatca ccagcttcga
     3001 ttccggaaca tgggccatcc cccgcctcac ctttctcgcg ggcacaaaaa aagcactcac
     3061 cgactccgtc cgcatcaccg taggatattc gaagatcgac cccgcaaagg attttcatga
     3121 tataaaggat atcgtcgaga tacccaaccc cttcgcccgc tggataccct ggatcatagc
     3181 agccgtcacc gtcctctccc tggtcctcgt cttctggctc atccgcaaac gaaagatcat
     3241 aaaacagctc gtcatccgcc agcttccccg gctctccccc ttcgaagaag ccatgcagca
     3301 actcgacgaa ctggcaaaac aacagctgcc ggtgaatgga gccataaaaa cctactacag
     3361 ccggctggac gagatctt
//
LOCUS       DATGFG010001185         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_874912, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001185 DATGFG010000000
VERSION     DATGFG010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874912"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..853)
                     /locus_tag="VKQ52_22465"
     CDS             complement(65..853)
                     /locus_tag="VKQ52_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90041.1"
                     /translation="MPKFTLQIVAFIDILGFSNMLPVFEQEALENDVIGEDGFHESET
                     LNKLVDIFKSAVKLVKNANCKSYQFSDNLCITINFINDGTENPELFVEMLRLISMLMY
                     EFVKEGYFLRGGVDAGWFLDADDIAVGMPLLTAYQLESKKAIYPRVLLSEEFLKILDE
                     FNRNHKLTDMAAFLAEYYIVREGTLGYLNPFFYITNFEEKESKIGYLKKYSEIIKEKL
                     ERFNHDAKIGQRYYWLATEFNEFIRRYITDEAYLELDDCKFWSC"
     gene            complement(1002..2285)
                     /locus_tag="VKQ52_22470"
     CDS             complement(1002..2285)
                     /locus_tag="VKQ52_22470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLZ90042.1"
                     /translation="MNNTRTTMHRGGFINILLIILAINFLLDGYVLSGLKTLTKGWRS
                     QRGRQLVKWGFLFISLGLTLTLLLGMGSFRTAKGMTPFHEWILSLFLALFFTKLFFCI
                     ILLLGDLGRVLAGLVNTLNKRHTGPALPSRRRFISQGAALIAAVPFGAFLYGMIKGKY
                     DYRVHRETLYFDDLPAAFDGFTITQLSDIHSGSFDNAAAVQRGIDLAKAQNSDLFVFT
                     GDLVNNAAWEIEPWLTRFAGIKAPYGQYSILGNHDYGDYIEWPDKQEKAANLQRLKAH
                     HKTLDYRLLLDEHVDIEKDGQKITLVGVQNWGRGFIQLGDLDKALEGADPHGFKICLS
                     HDPTHWEEKIRFHPTTIHLTLAGHTHGAQFGVESDKWRWSPVEYRYLDWAGLAQEKDR
                     YLYVNRGFGFLAFSGRLGIWPEITVITLKKKVRLA"
     gene            2517..>3433
                     /locus_tag="VKQ52_22475"
     CDS             2517..>3433
                     /locus_tag="VKQ52_22475"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLZ90043.1"
                     /translation="MGLFDKIRNEFIDIIEWVDTTSDTIVWKFPRYQNEIKMNSKLTV
                     RESQWAVFLNEGAIADVFQPGMYTLSTQNMPILTTLKGWKYGFNSPFKADVFFVSSRQ
                     FINQKWGTRNPIMLRDAEFGPIRLRAFGSFAFRVKDPAVFLKEVAATNPEFSVEAINE
                     QLRNLAVSRGMDAIAEAKIPVLDLAANYDEVSAKITDKIKPEFTELGLELTKFLIENI
                     SLPPEVEAALDKRSSMGIVGNLGAYAQFQAANAMEEAAKNPGGGGLAGVGIGAGLGMA
                     VGGQMGNVFANNSVQGNDGPPPLPGVTPF"
BASE COUNT          840 a          792 c          944 g          857 t
ORIGIN      
        1 cttgcatcaa gctcttactc ttattgcctg cttcgcaggt gggtcagcat cccagaatgg
       61 tgggtcaaca tgaccagaat ttacaatcat ccaattcaag ataagcttca tcagtaatat
      121 atctacgaat aaattcatta aactcggttg ctagccaata atatctctgg ccaatcttgg
      181 catcatggtt aaaccgctcc aatttttcct taattatctc tgaatatttt tttagatacc
      241 cgattttact ttccttttcc tcaaaattcg ttatataaaa aaatggattc aaataaccca
      301 atgtaccctc tcttacgata taatattccg caagaaaagc agccatatca gtaagcttat
      361 ggttccgatt gaattcatct aaaattttta aaaactcctc tgaaagaagc actcgcggat
      421 aaatcgcctt tttgctttcc aactgataag cagtaagtaa cggcatacca acagctatat
      481 catccgcatc taagaaccag ccagcatcaa cacctcctcg caaaaagtag ccttccttta
      541 caaactcata cataagcata ctgataagcc taagcatttc cacaaacaat tcgggattct
      601 ccgttccatc attaataaag ttgatagtaa tgcacagatt atcactaaat tgataggatt
      661 tacaattggc attctttacc aattttacag cgcttttaaa gatatccact agtttattca
      721 atgtttcgga ctcgtggaat ccgtcttctc ctattacatc gttctccaat gcttcctgct
      781 caaatacagg tagcatgtta gaaaaaccca aaatatcaat aaacgcaaca atttgaaggg
      841 taaatttagg catctttaag tattcaaaat catttgatta agattcatac ctagtaattg
      901 ttatccaatt ttttgcatat caaatttacc aatttttttg gccaaaggaa gaataataag
      961 cgataaaata tggctgtctg ctccacagcg ggtagaccga atcaagccaa tcgaaccttt
     1021 ttctttaatg taatgacggt gatctccggc cagatgccga ggcggcccga gaaggccagg
     1081 aagccgaagc cgcggttgac atagagatag cgatcctttt cctgcgcgag accggcccag
     1141 tccaggtagc ggtattctac ggggctccat cgccatttgt cggattccac accgaattgc
     1201 gccccgtggg tatgaccggc cagggtaagg tggatggttg tcgggtggaa gcggatcttt
     1261 tcctcccaat gcgtcgggtc gtgggagagg catattttga agccgtgggg gtcggcgccc
     1321 tctagcgctt tgtcgaggtc tcccagctgg atgaacccgc ggccccagtt ctgcacgccg
     1381 acgagtgtta tcttctggcc gtccttttcg atgtcgacgt gttcgtcgag gaggaggcga
     1441 taatccagtg ttttatggtg ggctttcagg cgttggaggt tggctgcctt ctcctgttta
     1501 tccggccatt cgatatagtc gccatagtcg tgattgccga ggatggaata ttggccgtag
     1561 ggcgctttta tgccggcgaa gcgggtgagc cagggttcga tctcccaggc ggcgttattg
     1621 accagatcgc cggtgaacac gaagagatcg ctgttctggg cttttgccag atcgatgccg
     1681 cgttgtacgg cggcggcgtt gtcaaagctg ccggaatgga tatcggagag ctgcgtgatc
     1741 gtgaagccgt cgaaggcggc agggaggtcg tcgaagtaga gcgtctccct gtggacgcgg
     1801 tagtcgtatt tgcctttgat catgccgtag aggaaggcgc cgaagggaac cgcggcgatg
     1861 agggcagctc cctggctgat gaagcggcgg cgggaaggga gggcgggacc ggtgtggcgt
     1921 ttgttgaggg tgttgacgag accggcgagg acgcggccca ggtcgcccag caggaggatg
     1981 atgcagaaga acagttttgt gaagaacagg gccaggaaga ggctgaggat ccattcgtga
     2041 aaaggggtca tgccttttgc cgtgcgaaag ctgcccatgc ccagcaggag ggtgagggtt
     2101 aatccgaggg aaatgaacag gaagccccat ttgacgagtt gccggcctcg ttgggagcgc
     2161 cagccttttg taagggtttt gaggccggag aggacgtagc cgtccaggag gaagttgatt
     2221 gccaggatga tcaacaggat atttataaag ccgccgcggt gcatggttgt tcgggtatta
     2281 ttcaatgatc catacaattt actatatgcc aggcaaaaaa cgcgttccgc accttctttt
     2341 tacgacgaat gcgtggaatg tgacgacgac ggcggaggcg ggcacagtga attggcggct
     2401 gcttttcaat aaaggatgcc gccgattgaa tatgaatcag ttagaatatt tttgatgact
     2461 ggtgttcccc atctagtccc tttcaattaa tttcacccag cttataaaat tgaaatatgg
     2521 gactttttga taagatcagg aacgagttta tcgatattat cgagtgggtc gatacgacca
     2581 gcgacaccat tgtctggaaa ttcccgcggt atcagaatga gatcaagatg aacagcaagc
     2641 tcaccgtgcg ggagtcgcag tgggcggtgt tcctgaacga gggggcgatc gcggatgttt
     2701 tccagccggg gatgtatacg ctgtcgacgc agaatatgcc gatccttacc acgctgaagg
     2761 ggtggaaata tggttttaac agccccttca aggcggatgt attctttgtc agcagccggc
     2821 agttcatcaa ccagaagtgg gggacgcgga acccgatcat gctgcgggat gcggagttcg
     2881 ggccgatacg gctgcgggcc tttggcagtt ttgccttccg ggtgaaggat ccggctgttt
     2941 ttttgaagga ggtggcggct accaaccccg agttctcggt ggaggccatc aacgagcagt
     3001 tgcgaaacct ggccgtttcc cggggcatgg atgcgatcgc cgaggcgaag atccccgtgc
     3061 tcgatcttgc cgccaattac gatgaggtga gtgcgaagat caccgataag ataaaacccg
     3121 agtttaccga actggggctg gaattgacta aattcctgat cgagaatatc tcgctgccgc
     3181 ccgaagtgga agcggcgctg gacaaacgca gcagcatggg gatcgtcggc aacctgggcg
     3241 cctatgcgca gttccaggcg gccaatgcca tggaagaggc ggcgaagaac cctggcggcg
     3301 gcggtcttgc cggcgtgggg atcggtgcgg ggctgggaat ggccgtcggt ggacagatgg
     3361 ggaatgtgtt tgccaacaat tccgttcagg gtaacgacgg gccgccaccg ctgcccgggg
     3421 tgacgccttt ttt
//
LOCUS       DATGFG010001186         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_418794, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001186 DATGFG010000000
VERSION     DATGFG010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418794"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VKQ52_22480"
     CDS             <1..446
                     /locus_tag="VKQ52_22480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLZ90044.1"
                     /translation="PGDTIYAARRGVVSAMDVSNTENDAGANTTTNWNYIEIFHADGS
                     FAEYGIIKKDGALVRPGQTVEAGAPIGIVGGDQYGRGAEIRFSVSYYPGVPNTQIPLQ
                     FWTKGNGRGQLKQGGFYTSEFTKALLTVETTKKPAGKAPAKKAVH"
     gene            569..1474
                     /locus_tag="VKQ52_22485"
     CDS             569..1474
                     /locus_tag="VKQ52_22485"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLZ90045.1"
                     /translation="MEHTQSPKDTHGEKPDWLPANLKKEMGHFNVFRLSDVLGKPHGP
                     MPYRRIDYFKISLMMGNNRLEYADKILEINSPSLVFSNPLIPYKWERLSPNPTGFFCI
                     FTEAFFRHFGHLRDYPLFQPGTIPVFTLSDEQTAAISHLYERMLTEIASDYAYKYDVI
                     RTLVFELIHHALKMQPANTSFYADSNAALRISSLFMELLERQFPIESPSQQVALRAPS
                     EYASQMAIHVNHLNRALRETSGKTTTKLIAARIVQEAKSLLRHTTWNIGEVAWCLGFE
                     ERSHFINFFKKNTRLTPGAWRESAI"
     gene            1627..>2104
                     /locus_tag="VKQ52_22490"
     CDS             1627..>2104
                     /locus_tag="VKQ52_22490"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLZ90046.1"
                     /translation="MEKKVWYITGASKGLGLALVKRLLADGHQVAATSRSLEALEEAA
                     GYPPADQFLPLEVDLSSEASVAGSLLTTHEVFGRIDVVVNNAGYGIGGALEELTIEEI
                     RDAFEVNIFAPIIVIQKVMPYLRTQRSGHIINIASIAGFAGVLGWSVYAATKQALIG"
BASE COUNT          470 a          802 c          457 g          375 t
ORIGIN      
        1 aacccgggga taccatctat gccgcccgtc gcggcgtcgt atccgcgatg gatgtaagca
       61 acaccgaaaa tgacgccggc gccaacacca ccaccaactg gaactatatc gagatcttcc
      121 acgccgatgg ctcctttgcg gaatacggca tcataaaaaa agacggcgcc ctcgtcaggc
      181 cgggccaaac ggtggaagcc ggcgccccca tcggtatcgt cggcggcgac cagtacggcc
      241 ggggcgccga gatccgcttc agcgtcagct actaccccgg cgtgcccaac acccagatcc
      301 ccctccaatt ctggacaaag ggcaacggca ggggacagct aaaacagggc ggcttctata
      361 ccagcgaatt cacaaaagcc ctcctcaccg tcgaaacgac caaaaaaccc gcaggaaagg
      421 cccccgccaa aaaagccgtc cactgatcgc cgacgccgcc atcctccagc ccgagccccg
      481 ccggccactg acagccgaca ccacccaagc cctgccggcg actgacaaca ctctgcgcgg
      541 ggatatttga ttttcgtatc tttgccccat ggaacacacg caaagcccga aagatacgca
      601 tggggagaaa ccggactggc tgcccgccaa cctcaaaaaa gagatgggcc atttcaacgt
      661 cttccgcctc agcgacgtcc tcggcaaacc ccatggcccg atgccctacc gccgcatcga
      721 ctacttcaag atctccctga tgatgggcaa caatcgcctc gaatacgccg acaagatcct
      781 cgagatcaac agcccctccc tcgtcttctc caaccccctt atcccctaca aatgggaacg
      841 actctcgcca aatcctaccg gcttcttctg catcttcacc gaagccttct tccggcattt
      901 cggccacctt cgcgactacc ccctcttcca gcccggcacc atccccgtct tcaccctttc
      961 cgacgaacag accgccgcca tcagccatct ctacgaacgg atgctgaccg agatcgcctc
     1021 cgactacgct tataaatacg atgtcatccg caccctcgtc ttcgaactga tccaccacgc
     1081 cctgaaaatg cagccggcca atacttcctt ctacgccgac tccaacgccg ccttacggat
     1141 ctcctcgctg ttcatggagt tattggaacg ccagttcccc atcgaatccc cctcccagca
     1201 ggtggcgctc cgcgccccct ccgaatacgc ctcccagatg gctatccacg tcaaccacct
     1261 caaccgcgcc ctccgcgaaa catccggcaa gacaaccacc aaactcatcg ccgcacggat
     1321 cgtccaggaa gccaaatccc tcctccgcca caccacctgg aacatcggcg aggtcgcctg
     1381 gtgcctcggc ttcgaagaac gctcccactt catcaacttc tttaaaaaga atacccggct
     1441 cacccccggc gcctggcgcg agagcgccat ctgatccccc cgcctacact tgccccggca
     1501 tctccgcccc ctccgcccaa tctcccctca atcgacattg tttgattttt gcacttattg
     1561 gtttgattct cgcatccgcc cgccctcccc gccgccctat ctttgccctt gcaaaaacga
     1621 ttaactatgg aaaagaaagt ttggtacatc accggcgcct cgaagggatt aggactcgcg
     1681 ctcgtcaaaa gactgctcgc cgatggccac caggtagccg ctacctcccg ctcactggaa
     1741 gccctggaag aagccgccgg ctacccgccg gcagaccagt tcctgccgct cgaggtcgac
     1801 ctctccagcg aagcctccgt cgccggctcc ctcctcacta cccatgaggt cttcggccgc
     1861 atcgacgttg tcgtcaacaa cgccggctat ggtatcggcg gcgccctcga agaactcacc
     1921 atcgaagaga tccgggacgc cttcgaagta aatatcttcg cccccatcat cgtcatccag
     1981 aaagtaatgc cctatctccg gacacaacgc tccggccata tcatcaacat cgcctccatc
     2041 gcaggcttcg ccggcgtcct cggctggtcc gtctacgccg cgaccaaaca ggcactcatc
     2101 ggcc
//
LOCUS       DATGFG010001187         4014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1033422, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001187 DATGFG010000000
VERSION     DATGFG010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033422"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VKQ52_22495"
     CDS             complement(<1..650)
                     /locus_tag="VKQ52_22495"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLZ90047.1"
                     /translation="MPKIAMLGSGFIGRFYADSILGQRSRDSIVSIYSRRKESAEKFA
                     KDYRCKHWTTEMEEAIAHPEADLVCIALPNNLHEIAVLLCCKHKKAVMTTKPLGRNAT
                     EARRMLEAVEDAGILNAYLEDLVYTPKFLKAHQSVAAGALGRILWAKSRETHPGPHSE
                     WFWDLEQAGGGCILDLGCHCVEIARSFIGKDIRPVEVMCWADTQVKPIEAEDHAIGL"
     gene            complement(694..1887)
                     /locus_tag="VKQ52_22500"
     CDS             complement(694..1887)
                     /locus_tag="VKQ52_22500"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HLZ90048.1"
                     /translation="MKFKTGSQLAFMMFVEYFIWGAWYVTMGSYMPANLHSSGLQIGA
                     AYSALAIATMISPFFVGLVADRYFPAQKIMGFMHLLGGVLLFLVPMISNNSVFYWTIL
                     LYSLLYMPTIALSNSVAFYQMTDPGKQFPWIRVFGTAGWIVAGIIIGKFELEKTATTF
                     QIAAGASVLLGLVSFALPNTPPKGAESRAPGTEAFVLFNNKPYLIFFIAAVLVCIPLS
                     FYYGFANNFLIELGMTNSAAKMTLGQFSEGLFILAIPFLFNRIGVKNMLLMGMTAWIL
                     RYICFAYGGLGANSWMLYAGIILHGVCYDFFFVTGYMYTEKKAGERIKNAAQQLFTFA
                     TYGLGMFIGTWFSGYIDDVYAISPNVHNWRGIWLVPAYIAAAVLIYFILFFREKKVLP
                     QTQNK"
     gene            1945..2601
                     /locus_tag="VKQ52_22505"
     CDS             1945..2601
                     /locus_tag="VKQ52_22505"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HLZ90049.1"
                     /translation="MRTFEDSYRHKGLRRQLVEVIRKKGITDERVLDAILHIPRHFFM
                     DSAFDKIAYEDRAFPIAEGQTISQPYTVAYQTQLLEVKPFDKVLEIGTGSAYQACVLA
                     EMRAQVYTIERQKKLFDGNKQFPFLKKYPALKFFYGDGYEGLPTYGPFDKVIVTAAAP
                     FIPPKLVEQLKPGGLMVIPVGEGAVQRMLRLTKQADGSATQEVYDNFSFVPMIEGKNS
                     "
     gene            complement(2697..3545)
                     /locus_tag="VKQ52_22510"
     CDS             complement(2697..3545)
                     /locus_tag="VKQ52_22510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLZ90050.1"
                     /translation="MKISQKLALRYARARINILSLASPRKAAIKAFKLFCTPQQRVSK
                     KGSAFFANGEPLAFRHAGHTVRGHRWLPAELPRKKVLIAHGFESASRNFETYIAALVA
                     KGYEVVAFDAPAHGASGGRRILLTDYVSLLRTIEQNYGPFHSYMGHSLGGLALALFLE
                     NNPHNKVTRLALIAPAVDTTLAVGVFARLLQLSPEVVREMDDYVQETSGHHFAWYSLR
                     RALHHISANILYLQDEEDRITPIKEALLVQQDKHPNVRFVFTKGLGHRKIYKDQETME
                     RIVGFL"
     gene            3565..>4014
                     /locus_tag="VKQ52_22515"
     CDS             3565..>4014
                     /locus_tag="VKQ52_22515"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLZ90051.1"
                     /translation="MKTVIIGSGNVATVLGGRIAAAGHTILQVVARREAAALSLATEW
                     ECGYTTRWEETDPGADIYIVALSDRVIPELGRVLSLPGRLVVHTAGAVPRSVLQEISE
                     RTGVLYPLQSLRKEIRPFPEFPLLIDAGRPDDLAVIDAFARTLARQVE"
BASE COUNT          887 a          987 c         1299 g          841 t
ORIGIN      
        1 aggccgatgg cgtggtcttc cgcttcgatg ggttttacct gggtgtcggc ccagcacatg
       61 acctcgacgg ggcggatatc cttaccgata aagctcctgg cgatctccac gcaatgacag
      121 ccgaggtcga ggatacagcc gccgcccgcc tgttccaggt cccagaacca ttcgctgtgc
      181 gggccggggt gggtctcgcg ggatttggcc cataggatcc ggccgagggc gccggccgcc
      241 acgctttgat gcgccttcag gaatttcggt gtatagacga ggtcttcgag ataggcgttg
      301 agtatgccgg cgtcctctac tgcttccagc atgcggcggg cctcggttgc gttacggccg
      361 aggggttttg tggtcatcac ggcctttttg tgtttgcagc agagcaggac ggcgatctcg
      421 tggagattat tgggaagggc aatgcagacc aggtcagcct ccgggtgggc gatggcctct
      481 tccatttccg tggtccagtg cttgcaacgg tagtctttgg cgaatttctc cgcgctttct
      541 ttccggcggg agtagatact gacgatggaa tcgcggctcc gctgacccag gatcgagtct
      601 gcgtaaaagc ggccgataaa accggagcct aacatggcaa tctttggcat aaaatgagat
      661 ttttacagat tatcctttta aaaaattgtc gatttatttg ttttgtgttt ggggcagcac
      721 ctttttctcc cggaagaaga ggatgaaata aataagcacg gcggcggcga tataggccgg
      781 tacgagccag atgccgcgcc agttgtgcac attcgggctg atcgcatata cgtcgtctat
      841 atagcccgag aaccaggtgc cgatgaacat cccaagaccg taagtggcaa aggtgaagag
      901 ctgctgggcc gcattcttta ttctttcacc agccttcttc tcggtgtaca tatagccggt
      961 gacgaagaag aagtcgtagc agacgccgtg gaggatgata ccggcataga gcatccagct
     1021 gttggcgccg aggccgccat aggcaaagca gatatagcga aggatccagg cggtcatacc
     1081 catgagcagc atgttcttta cgccgatgcg gttgaacagg aaggggatgg ccaggatgaa
     1141 gaggccctcg gagaactggc caagggtcat cttggcagcg gaattggtca tacccagttc
     1201 gatgaggaag ttattggcga agccgtaata gaaggacagg gggatacaga cgaggacggc
     1261 ggcaataaag aagatgagat agggtttatt gttgaacagg acgaaggctt ctgtgccggg
     1321 ggcccgggat tccgcgcctt ttggcggggt attgggaaga gcgaagctga cgagacccag
     1381 caggacggag gcgccggcgg cgatctgaaa ggtggtggcc gtcttctcga gttcgaattt
     1441 gccgatgatg atccccgcta cgatccagcc cgccgtaccg aagacccgta tccaggggaa
     1501 ttgttttccc gggtccgtca tctggtagaa ggcgacgctg ttggagagtg cgatggtggg
     1561 catgtagagc agggaataca ggaggatggt ccagtaaaag accgaattgt tgctgatcat
     1621 gggtactaaa aacagcagga caccacccag caggtgcatg aaacccatga tcttctgcgc
     1681 cggaaaatac cggtcggcga ccaggccgac aaagaagggg gagatcatgg tggcgatggc
     1741 cagcgcgctg taggccgcgc cgatctgcag gccggacgaa tgcaagttgg caggcatata
     1801 ggagcccatc gtcacatacc aggcgcccca gatgaaatat tcgacgaaca tcatgaacgc
     1861 caactggcta ccagttttaa atttcatttt agttttagtt gtggattagg tggttcgaat
     1921 ataggcatta aattgcaccc gtttatgaga acattcgaag acagttaccg ccataagggt
     1981 ctgcgcaggc agctggtgga ggttatccgg aagaaaggca ttacggatga gcgggtattg
     2041 gacgccattc tccatatccc gaggcatttc tttatggatt ctgcatttga caagatcgct
     2101 tacgaggacc gggccttccc gatagccgag gggcagacca tctcacaacc ttatacggtg
     2161 gcctaccaga cgcagttgct cgaggtgaag ccctttgaca aggtgttgga gatcgggacg
     2221 ggcagcgcct accaggcttg tgtactcgcg gagatgcggg cgcaggtgta tacgatcgag
     2281 cggcagaaga aattattcga cggcaacaaa caatttcctt ttctcaaaaa atatcccgca
     2341 ttgaaattct tctatggcga cggctatgag gggttaccca cctatggacc tttcgacaag
     2401 gtgatcgtca ccgccgccgc gccgttcatc ccgccgaagc tggtggagca gctgaagccc
     2461 ggcgggctga tggtgatccc ggtcggggag ggcgccgttc agcggatgct gcggctgacg
     2521 aagcaggccg atgggagcgc gacgcaggaa gtgtatgata atttctcctt tgtgcccatg
     2581 atcgaaggga agaacagctg aggcggcgcc ccttcgccaa aagcaggtat ggtcggcgaa
     2641 agccgatgat gcgagccggt atggtcaaag gaatccgacg atgcgttcca gtacggtcag
     2701 aggaagccga ctatgcgttc cattgtttcc tggtccttat agatcttgcg atgccccagt
     2761 ccctttgtaa agacgaagcg gacattgggg tgtttgtcct gctggacgag cagggcttct
     2821 tttatcggcg tgatcctgtc ctcttcgtcc tgcaggtaga ggatattcgc agagatatgg
     2881 tgaagggccc ggcggaggga ataccaggca aaatgatggc cgcttgtctc ctggacatag
     2941 tcatccattt cccggaccac ttcgggggag agctggagca ggcgggcgaa gactcccacg
     3001 gccagggtgg tatcgacggc aggtgcgatc agggccagcc tggtcacttt gttatgcggg
     3061 ttgttctcga ggaaaagagc gagagcaagg ccaccaaggg aatgccccat ataggaatgg
     3121 aaggggccat agttctgctc gatggtgcga aggaggctga cgtaatcggt gagcaggatg
     3181 cggcggccgc cggaggcccc gtgcgccggg gcgtcgaagg caaccacctc atagcctttg
     3241 gccacgaggg cggcgatata ggtctcgaaa ttccgggagg ccgattcgaa gccatgggcg
     3301 atgagcactt tcttgcgggg tagttcggcg ggaagccagc ggtgaccgcg tacggtgtgg
     3361 ccggcatgac ggaaagcgag gggctcgccg ttcgcgaaga aagcggaccc tttttttgat
     3421 acccgctgct gcggggtaca gaaaagctta aaggccttta tggcggcctt gcggggggaa
     3481 gcgagggaaa gaatgtttat cctggcacgt gcataacgga gtgccagttt ttgcgatatc
     3541 ttcatgatgc cgtaaaatta cagaatgaaa acagtaatca tcggatcggg aaatgtggcc
     3601 accgtactgg gaggaaggat cgccgccgcg ggacatacca tcctgcaggt ggtggcaaga
     3661 cgggaagcgg cagcgttgtc actggcaacg gaatgggaat gcggatatac gacccggtgg
     3721 gaggagaccg atcccggggc ggatatctat atcgtcgcgc tcagcgaccg ggtgataccg
     3781 gaactgggcc gggtgttgtc gctgccgggc cggcttgtcg tgcacacggc gggggcggtt
     3841 ccccgttcgg tcctgcagga gatcagcgag cgtacggggg tgctttatcc cctgcaaagc
     3901 ctgcgaaagg agatccggcc tttcccggag ttccccctgt tgatcgatgc cggcaggccg
     3961 gacgacctgg ccgttatcga cgcgtttgcg cgaacccttg cccggcaggt ggag
//
LOCUS       DATGFG010001188         5690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_127101, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001188 DATGFG010000000
VERSION     DATGFG010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127101"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="VKQ52_22520"
     CDS             complement(<1..1767)
                     /locus_tag="VKQ52_22520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90052.1"
                     /translation="MKYPDLRSLTRIDTALIYRTVTNKMISDFYYDPTDVTTADDSAM
                     TIVTLAGKRLKRYTQGVLYPRWFGAAADGITDDSYAIQKMFNYITLTNATHYWKVYFE
                     GLTYNIGDGIDLPMLLADGSTSLPHIDIDGGGAVIKTSHPKAMFRRVPGNDAMAQSLI
                     SSWIGEIHNFEFRGTQASGQVALDLGACYSWHIHNNHFMTLDTAIKARFFLQDIIAHN
                     RFTNNRSICIWMKWGDWTGGTTAGDANNADIIESNRFYNWDSAYAALYMLAGNKNTIR
                     NNVSEGNKPVYEFFYDYSGSTNVVSTEIHDWYSETVPGKIAVNTLFNIRMSGTFDIDN
                     VTSIYPCVLLNLDNSGTGSILNVDNFNFYEVADTPFSARQAGLYGLNIGKTGKALFNL
                     VNAASHWSGNVIPAIGIMYVGANQDGSTIYTAGAGAAQINGRLVVGAETDLNGYLVAN
                     STLYANGYTYHNYNVFMNNAPIVFQNSSGSGSSIGARLTDSSFYIGSQNGWFSKQGKN
                     GNWLLNTKNIDAPTPVALSVLGTDAIKIPLGNTAQRPTGDSAYYRFNRDSLTNELHDG
                     GSWQAIATRNWVRANSSANVFKK"
     gene            2285..2440
                     /locus_tag="VKQ52_22525"
     CDS             2285..2440
                     /locus_tag="VKQ52_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90053.1"
                     /translation="MKLIIQIKEKNYFPYENKIKMAAAGESLYSVKRERMGRWAKGKN
                     NDVDAGD"
     gene            complement(2453..3541)
                     /locus_tag="VKQ52_22530"
     CDS             complement(2453..3541)
                     /locus_tag="VKQ52_22530"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HLZ90054.1"
                     /translation="MLISSAARRSPALLLLILAFNSPAQNITTPRTPSPAATVLQTIG
                     ISTVSVNYSRPSVKGRIIWGNLVPYGYNKQGFGAGNEAPWRAGANENTVIRLSHPGKI
                     EGHPVPAGEYGLFFVVNQDNTGEVILSKDSRSWGSFWYVPDHDLFRAKIALREIPMTE
                     MLTYDFQSLTKTSAELELNWEKKQFPVRIEFDVDEIVMANAVEELKGPVGFNWQGYAS
                     AANYALANKVNLPQATLWIDRAVQISPNFNTLSTKAKLLKATGNSGQYDSLMREALAV
                     GSENDLNNFGYQLLNDKSYDRAIEVFILNTQKHPKSANTFDSLGEAYATKGDKKNAIL
                     CFKKSLSLNPADATRQNSEKYLKQLGAL"
     gene            complement(3720..>5690)
                     /locus_tag="VKQ52_22535"
     CDS             complement(3720..>5690)
                     /locus_tag="VKQ52_22535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLZ90055.1"
                     /translation="NRIYKNVAPYVAANQQWNTLSGYPVLQEDNNTPFMAIGTDLNNP
                     GDNTNATNILAVTSGYLFYYDGQHLKAFNKATGATAGTPVSFPWTALMQQGIYADECS
                     NVYIGAADGTIKVFRFNGTLFDDNAVADIPIPGYAGKAVYSLAYDQTHNLLYAGGDGF
                     VSAFDLSGYCAAPTPPPATYTIVVSPGATSVSASITPAPPAGAVVTYTLLQGTTVIGT
                     NATGAFTNLDPQADYTMRADIVLGCANAQAIANFKLKLDVELQATDACGTTGGTVTAT
                     ASGGAIPYSYSKNGIDFQQSNVFPHLGAGTYTIVVMDYSGDMASQTVTITANDLVVRT
                     GNDTVICEGTTAILSGSTNGTNFAWSPSAGLSSTSVTNPVAAPVETTKYYFSATRGDC
                     TLTDSTTVMVLQAPVANAGNDTSICYGTIGILQGSGGVSYSWTPSTFLNNDTIANPVV
                     SGALHSVVYTLQVTDGSGCRSIRDDTVVLRVVPPAKVYAGKDTIVAIGEPLQLNAIDL
                     NHSGFDHYTWSPPDGLNDALSQDPVAVVENSVTYEVRAVTPEGCAAENTIKITAYKGP
                     TIYVPNSFTPNGDGHNDVLRLVMPGMATLRYFIIFNRWGQEVFHTTNPSMGWDGRLNG
                     AGQAAGVFVWTVEAVDYTGKVIQKKGTVLLIR"
BASE COUNT         1330 a         1358 c         1550 g         1452 t
ORIGIN      
        1 tttcttaaac acattggcac tggaattggc cctgacccag ttgcgcgtag cgatggcctg
       61 ccaggagcca ccgtcgtgca gctcattggt cagcgaatct ctattgaacc ggtaataagc
      121 gctgtcgccc gttggccgtt gggcggtgtt cccgaggggg attttaatcg cgtcagtgcc
      181 aagcacgctg agggccacgg gcgtaggggc atctatattt ttggtgttca ggagccagtt
      241 cccgtttttg ccttgcttcg aaaaccatcc gttttggcta ccgatataga aggaagagtc
      301 tgtcagtctt gctccgattg aacttcccga acctgacgag ttttggaaaa caatcggggc
      361 attattcata aaaacattat agttatgata agtatagcca tttgcataaa gggttgaatt
      421 ggcaaccagg tatccattga ggtctgtttc tgcacctact accagtctgc cgttaatctg
      481 ggccgccccg gcgccggccg tgtatatggt gctcccgtcc tggttggcgc ctacatacat
      541 aataccaatg gcgggaatga cattgccgga ccaatgactc gccgcgttta ccaggttaaa
      601 caatgctttg cctgttttgc cgatatttaa tccatacaaa cctgcctgcc gggccgaaaa
      661 cggggtatca gccacctcgt aaaagttaaa attatcgacg ttcaggatgg aaccggtacc
      721 actgttatcg aggttgagca aaacacatgg ataaatggag gtaacattgt ctatatcgaa
      781 tgtacccgac atccggatgt taaaaagggt gtttacggcg atcttgccgg gaacggtttc
      841 ggaataccag tcatggatct ccgtggatac aacattggtg ctgccgctat agtcatagaa
      901 gaactcatat accggcttgt tcccttcaga gacgttgttt cttatggtgt tcttattgcc
      961 cgccagcata tacaaggcgg cataggcgct gtcccaatta taaaacctgt tgctttcaat
     1021 tatatctgca ttgtttgcat caccggccgt ggttccaccg gtccagtcgc cccatttcat
     1081 ccagatgcag atggatctgt tgttggtaaa gcggttgtgg gcgataatat cttgtaaaaa
     1141 gaacctggct tttatcgccg tgtccagggt catgaagtgg ttgttatgga tatgccagga
     1201 gtaacaggcg cccaggtcca gtgccacctg cccgctggcc tgtgtgccgc ggaactcgaa
     1261 gttgtggatt tctccaatcc agctgctgat caatgactgg gccatggcat cgtttccggg
     1321 cacccgtctg aacatggctt tcggatggct tgttttgata accgctccgc cgccgtcaat
     1381 atcgatgtgc ggcaggctgg tgctaccatc cgcgagcagc atcggcaggt caatgccatc
     1441 cccgatatta taggtaagcc cttcaaaata gactttccag taatgggtgg cattggtcaa
     1501 tgtgatatag ttgaacattt tttgaatggc atagctgtca tccgtgatgc catctgccgc
     1561 agccccgaac caccggggat aaagcacgcc ttgtgtatac cgctttagcc ttttcccagc
     1621 cagggtaaca atggtcatgg cgctatcgtc tgccgtggtg acatcggtag ggtcataata
     1681 aaaatccgag atcattttat tggtgacggt tctatagatc agtgcggtat ctatcctggt
     1741 gaggctgcgt agatccgggt atttcatgac aacaaattcc ttggtcgcga tcttattggt
     1801 tccgtcgttt acaatggtga tattatctat agcccaggat tgcactttta atgtgtccgg
     1861 gccgatcagg aaaatgctgt cgtttatttt cgccagtact cttttaaata tggttcgccc
     1921 gttacccttg ttaaagagga atccgggaac gttttgcgtg tgattttcaa taattagctc
     1981 ggctgagtca caattagtta ttttgacgct gtcagctttg atagtataga cgttttgagc
     2041 aaaaaggctc cggtgcagaa ggcacactat caccagtaaa agaattcttt tcatttataa
     2101 ggttttattg attgacacga agagctggtc acgcatcatc gtttggatgc cgcgggaaat
     2161 gaaattcggg aagttaacgg aggaaaaccc agttcggggg ggacgggcaa aaacatggct
     2221 ctgccacatt caaaaaatgg ttcatagtta ggttgtaaag gttaatgaaa gtctataggg
     2281 caggatgaaa cttattatcc aaattaagga aaaaaactat ttcccatatg aaaataaaat
     2341 caagatggca gcggcaggcg aaagcctata ttccgtcaaa agggaaagaa tgggccgttg
     2401 ggcaaaaggc aagaacaacg atgtggacgc gggtgattga gatgagtgcc ggttacaggg
     2461 cgcccagttg tttcaggtat ttctctgaat tctgccgggt cgcatcggcg ggattcaggc
     2521 taagtgactt tttgaaacag agaatggcat tcttcttgtc gccttttgtg gcataggctt
     2581 cgccgaggct gtcgaacgtg ttggcgcttt ttggatgctt ttgggtattc agtataaaga
     2641 cttcgatggc gcggtcgtag gatttgtcat tcagcaattg atagccgaaa ttgttcaggt
     2701 cattctcgga gccaacggca agcgcctccc gcatcaggct gtcgtattgc ccggagttgc
     2761 cggtggcctt cagcaatttt gccttggttg agagcgtatt gaagttgggg ctgatctgga
     2821 cggcccggtc gatccacaac gtggcctgag gaaggttcac cttgtttgcc aatgcataat
     2881 tcgcggcgct tgcatagcct tgccagttga atccaaccgg tcccttcaat tcttcgacgg
     2941 cgtttgccat cacgatctca tcgacatcga attcaattct caccggaaat tgtttctttt
     3001 cccagtttaa ttccagctcc gccgaggtct ttgtcaggga ttgaaagtca taggtgagca
     3061 tttccgtcat cgggatctcc ctcagtgcga tctttgcgcg gaagaggtcg tgatcgggca
     3121 cataccagaa gcttccccag gagcggctgt ctttcgacag tataacctcc ccggtattgt
     3181 cttgattgac gacgaagaag agcccgtact cgccggccgg aacgggatgc ccttcgatct
     3241 taccggggtg tgaaaggcgg atcactgtat tctcattggc cccggcgcgc caaggtgctt
     3301 cgttgcccgc gccgaaaccc tgcttattgt agccataggg aaccaggttg ccccatatta
     3361 tgcgaccctt gacggaaggc cgggaatagt taacactgac ggtggagatg ccaatggtct
     3421 gtaagacggt tgcggcgggg cttggtgtcc ggggggtggt gatattctgt gcgggggagt
     3481 tgaatgcgag gatgagcagg agcagggcgg ggctgcgcct ggcggcggat gatattagca
     3541 tgatagttgg tttatcttaa gatacgaatt accggatgcc aatgaaggtt tggcgaaccg
     3601 aagcgggatt ttcgatgacc gaaggaggag gatgattgtc cgggggagga tgattgtccg
     3661 aggggaggat aattgtccgg ggccgggctg accgacgcgg gcggctggct ccgggaacct
     3721 taccggataa gcaatacggt accttttttc tgaatcactt tacccgtata atccaccgct
     3781 tcgaccgtcc acacaaaaac ccccgcagcc tgcccggcgc cattcaggcg accgtcccat
     3841 cccattgacg ggtttgtcgt atgaaatact tcctgccccc accggttaaa aataataaag
     3901 tagcgcagtg tcgccatgcc cggcattacc aatcgaagaa cgtcattgtg gccgtcccca
     3961 tttggagtga aagaattggg tacatagatg gtcgggcctt tatacgctgt gatcttaata
     4021 gtattttctg cagcacagcc ctctggtgtc actgcccgta cttcataggt gacgctgttt
     4081 tcgacgactg caaccggatc ttgcgataac gcatcattca aaccatccgg cggagaccag
     4141 gtgtagtgat caaacccact atgattcaag tcaatggcat ttaattgcag cggctcgccg
     4201 atggctacga tcgtgtcttt gccggcgtaa actttggccg gagggacgac tctcaggact
     4261 accgtatcat cgcgaatgga gcggcaacct gatccatcag tgacctgcag ggtatagacg
     4321 acagaatgca aggcaccgga aacgacagga ttcgcaatcg tgtcattgtt caggaaagtt
     4381 gaaggcgtcc aggaatagga gacgccacca ctgccctgca ggataccaat tgtcccataa
     4441 caaatgctgg tatcattccc tgcgttggca acgggtgcct gcagaaccat gacggtcgtg
     4501 gagtcggtca aggtacaatc gcccctggtg gctgaaaaat aatatttagt ggtctccacc
     4561 ggcgccgcta ccgggtttgt gacgcttgta ctgctgagac cggcggaagg gctccaggcg
     4621 aaatttgtcc cattggtgct cccggaaagt atagcagttg tcccttcaca tatgaccgtg
     4681 tcatttcctg tcctgaccac caggtcgttg gcagtgatag taacggtttg ggaagccata
     4741 tctccggaat agtccatcac cacaatcgta taggttcccg ctcccagatg ggggaataca
     4801 ttgctttgct ggaaatcgat gccgttctta ctgtaggaat atggaatagc accaccggaa
     4861 gctgtcgcgg tgacggttcc gcccgttgtt ccgcaagcat cggtggcctg tagttcgacg
     4921 tcgagcttca gcttgaaatt ggcgatggcc tgcgcgttgg cgcaacccaa aacgatatcg
     4981 gcgcgcatag tgtagtcagc ctgcgggtca aggttcgtaa acgcaccagt ggcattggtg
     5041 ccgataacgg tcgttccctg cagcagcgtg taggtaacga cagcgcctgc gggaggagcc
     5101 ggggtgatgg aagccgaaac ggaagtagca ccaggggaaa cgacaatggt atacgtggca
     5161 ggcggcggag tcggcgccgc gcaataaccc gacaggtcga atgccgagac aaatccatcg
     5221 ccaccggcat atagaaggtt gtgggtctga tcatatgcca gggagtacac tgctttgccc
     5281 gcataaccgg gaataggaat atcggccacc gcattgtcgt cgaacagcgt tccgttaaaa
     5341 cgaaatacct ttatcgttcc atccgcggcg ccaatgtaca cattgctaca ttcgtccgca
     5401 taaataccct gttgcatcag ggccgtccat gggaaggaaa ccggtgtgcc ggccgtagct
     5461 cccgtcgctt tattaaatgc tttcagatgt tggccatcat aatagaacaa atagccggaa
     5521 gtaacggcca gtatattggt ggcattggta ttgtcgccgg gattattgag atcggtgcca
     5581 attgccatga agggcgtgtt attgtcctcc tgtaatacgg gatagcctga caacgtattc
     5641 cattgctgat tggcggcgac ataaggagct acattcttgt atatacggtt
//
LOCUS       DATGFG010001189         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_645082, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001189 DATGFG010000000
VERSION     DATGFG010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645082"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VKQ52_22540"
     CDS             complement(<1..688)
                     /locus_tag="VKQ52_22540"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HLZ90056.1"
                     /translation="MIKTIGVCGAGTMGSGIAQVAAMGGYRTLLYDVDAQMVQKGRIA
                     IEKSLQTLVEKKKLGPEEQQAILHRLSWSADLRDCVADLLIEAIIEQPDAKTRLFQQL
                     AQRNPDHTILASNTSSLSLDTLATAIPHPHRFIGMHFFNPAPLMKLVEIVNTKHTDAH
                     TTTAIMEVARQMGKTAVLCKDAPGFIVNHVARPYYLEALRLVEAGIGDIETIDTLMEA
                     SGFRMGPFRLM"
     gene            complement(710..1741)
                     /gene="pheS"
                     /locus_tag="VKQ52_22545"
     CDS             complement(710..1741)
                     /gene="pheS"
                     /locus_tag="VKQ52_22545"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLZ90057.1"
                     /translation="MEQLLQQIEQYRQEIAAIVPNGEESLEAYRIRFLGTKGIVKNLF
                     TEMKNVPGDKKREFGQILNEFKQLAESTYENWKSRLDTGAAGTARSIDLSLPGDALPL
                     GTHHPITLVRDRIVHIFGRLGFSVAEGPEIEDDWHNFTALNLPENHPARDMQDTFYIH
                     QNPDWLLRTHTSSVQARTMERQKPPIRIICPGRVYRNETISARAHCFFHQTEGLYVDE
                     NVSFADLKQTLYFFVQELFGKDVRIRFRPSYFPFTEPSAEMDINCRICGGSGCSVCKH
                     TGWVEILGSGMVHPKMLDNFGIDSNKYTGFAFGMGIERICQLQYRVNDLRLYSQNDVR
                     FLKQFTGAV"
     gene            1793..2929
                     /locus_tag="VKQ52_22550"
     CDS             1793..2929
                     /locus_tag="VKQ52_22550"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HLZ90058.1"
                     /translation="MSPNLHISDFLDPISLAQLSQDQGYRDGQIGKLIDVYDETFPDV
                     YLADIVLVGCEEERGIGRDRREPGGPDTIREQFYQLYYWHPDIKLADVGNIRTGASLN
                     DTYAALKTVLRELTAIGKTAVILGGSHDLTLAQYYTYSDKKHIIEATGVDALIDLDIH
                     SRNRSENFLMEMLTGEPNYIHHYNHIGFQSYYVHPHMLETMDKLRFDCFRLGSVKDNI
                     EEMEPVIRNSHLFTFDISAIAHSFAPANLLSPNGFNGEEACILMRYAGLSHNVNTVGI
                     YGYMAGKDHDALTARQISHMLWYLIDGKSRGRREAALSDRDFFVEYRTAFAEVETTFL
                     QSKKTQRWWMQLPDNKYIACSYKDYLLASSNEIPERWLRAQERG"
BASE COUNT          657 a          812 c          948 g          723 t
ORIGIN      
        1 ccatcaggcg gaaggggccc atccggaagc ccgatgcctc catgagggtg tcgatcgttt
       61 cgatatcgcc aatgccggct tccacgagac gcagggcttc gagatagtag gggcgggcca
      121 catgattgac gataaagccg ggcgcgtcct tacaaagaac ggcggtcttg cccatttgcc
      181 gggcgacctc catgatggcc gttgtcgtat gcgcgtcggt gtgttttgta ttgacgatct
      241 ccaccagttt catcagcgga gcggggttga agaagtgcat gccgataaac cggtggggat
      301 gggggatcgc ggtggccaat gtatcgaggg ataaggaaga tgtattggat gcgaggatcg
      361 tgtggtcggg attccgttgg gccaattgct gaaagagccg ggtcttggca tcgggttgtt
      421 cgatgatggc ttctatgagc agatcggcga cgcagtcccg gagatcggcc gaccaggaga
      481 gccggtgtag gatggcctgt tgttcctccg gcccgagttt tttcttttcc acgagggtct
      541 gcaggctttt ttcgatggcg atcctgcctt tctgcaccat ctgcgcgtcg acgtcgtaca
      601 ggagggtccg gtagccaccc atcgccgcta cctgggcgat gccgctgccc atggtgcctg
      661 ctccacaaac gccgattgtt tttatcatat ctggatttta agaagccggc tacaccgccc
      721 cggtaaactg tttcaggaac cggacatcat tctgggaata gaggcgcagg tcgttgacgc
      781 ggtattgcaa ctggcagatg cgttcgatgc ccatcccgaa ggcgaagccg gtgtatttgt
      841 tgctgtcgat gccgaagttg tccagcattt tggggtggac cattccgctt cccaggatct
      901 ctacccagcc ggtgtgtttg cagacggaac aaccgctgcc gccgcagata cggcagttga
      961 tatccatctc ggcgctgggc tccgtgaagg ggaagtaaga gggacggaaa cgaattctta
     1021 catccttgcc aaacagttcc tggacgaaga agtaaagggt ttgtttgaga tcggcgaaag
     1081 atacgttctc gtcgacgtac aggccttccg tctggtggaa gaagcagtgg gcgcgggcgc
     1141 tgatcgtctc gttacgatag acccggcccg ggcagatgat gcgtatcggg ggtttctgcc
     1201 gctccatggt gcgggcctgg acgctgcttg tatgggtgcg aagcagccag tcggggttct
     1261 gatggatata gaacgtgtcc tgcatgtccc gggcgggatg attctcgggc aggttgaggg
     1321 cggtgaaatt atgccagtca tcctctattt cggggccttc cgccacggaa aaccccagtc
     1381 ttccgaagat gtggacgata cgatcgcgta caagggtaat gggatgatgg gtgcccaggg
     1441 gcagcgcgtc accgggcagg ctgagatcga tgctccgtgc cgtgccggcg gctcccgtat
     1501 cgaggcggct tttccagttc tcgtaggtgg actcggccaa ttgtttgaac tcattcagga
     1561 tctggccaaa ctcccttttt ttgtcaccgg gaacattctt catttcggtg aacaggtttt
     1621 tcacgatacc ttttgttccc aggaaccgga tacggtaggc ctccagcgac tcctccccgt
     1681 tggggacgat ggcggcgatc tcctgcctgt actgttcaat ttgttgtaac aattgctcca
     1741 tggcgcaaag ataatttatt tttaatgtag cttgcctccc aaaacatcat ccatgtcgcc
     1801 caacctgcat atcagtgatt tccttgatcc tataagcctg gctcaactgt cgcaggacca
     1861 ggggtatagg gacgggcaga tcgggaagct gatcgatgtc tacgacgaga ccttccccga
     1921 tgtctatcta gccgatatcg tcctcgtcgg gtgcgaagaa gagagaggta tcggccgcga
     1981 ccggcgggaa cccggcggcc ccgacaccat ccgggagcag ttctatcagc tgtattactg
     2041 gcatcccgat atcaagctgg ccgatgtggg gaatatccgc accggcgctt ccctcaacga
     2101 cacctatgcc gcgctgaaaa cggtgctgcg ggaactgacg gccatcggca agacggcggt
     2161 tatcctgggg gggagtcatg atctcacgct cgcccagtat tatacttatt ccgataagaa
     2221 acatatcatc gaagccaccg gggtggatgc acttatcgac ctggatatcc attcccgtaa
     2281 ccggagtgag aattttctca tggagatgct tacgggggag cccaattata tccatcacta
     2341 caaccatatc ggttttcaga gctattatgt ccacccgcat atgctggaga cgatggataa
     2401 attacggttc gactgtttcc ggctggggag tgtaaaagat aatatcgagg agatggagcc
     2461 ggtgatccgg aattcgcatt tgttcacctt cgatatttcg gcgatagcgc attcctttgc
     2521 gccggccaac ctgctttcgc caaacggctt taacggggaa gaggcctgta tcctgatgcg
     2581 ctatgcgggg ctgagccata acgtcaatac ggtgggtatt tacgggtata tggcggggaa
     2641 ggaccatgac gcgctcacgg caaggcagat cagtcatatg ctctggtatc ttatcgacgg
     2701 gaagagcagg ggcaggcggg aggcggcgct ttccgaccgg gatttttttg tcgaataccg
     2761 cacggccttt gcggaggtgg agaccacctt tctgcagagc aagaagacgc agcggtggtg
     2821 gatgcagctg ccagataata aatacatcgc ctgctcctac aaggattacc tgttggccag
     2881 cagcaatgag atacccgagc ggtggttgcg ggcgcaggaa agaggctaga acccctggaa
     2941 ggggttcgcc cgaagggcag acattggctt cctgcgcgaa tagaatattc gtgcccaaaa
     3001 catatatcat gaaaaaatac tcaaccgtgt tacttctggc cgccgcctgc tggtggcagg
     3061 cctgtacgac gccgcatgcc gcaaccggcg cgccttccgc cgcagctgcc gcgggaccga
     3121 agttttcttc gaaggagccg
//
LOCUS       DATGFG010001190         4234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9722 k141_1217567, whole genome
            shotgun sequence.
ACCESSION   DATGFG010001190 DATGFG010000000
VERSION     DATGFG010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965291
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:40:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,492
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217567"
                     /isolate="SMAG_U9722"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1930
                     /locus_tag="VKQ52_22555"
     CDS             <1..1930
                     /locus_tag="VKQ52_22555"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLZ90059.1"
                     /translation="VYATSARHRVYAISAMTGQLRWSFDPFDGAEGGGVSRGVTFWQD
                     GADRRILFTGGDNLFALDANTGKPIPSFGNNGRVSMNIGIRDDPNTISIKPTSPGIVY
                     HDLLIIGNEVSELYGAQPGYVRAYAIRTGKLVWTFHTLPHPGEPGYETWPPDAWKYAG
                     GANDWAGMSLDERTGTVFLALGSPSYDFYGADRKGQNLYGNCIVALDAATGKHRWHFQ
                     TIHHDLWDYDLPAPPNLVTVTKNGKPIDAVAQTSKVGFLYILDRETGTPLYPIEEHSV
                     PASDVPGEEAWPTQPFPQKPAPYARQSMTKDDLADFSPAAHDSLVNSFRSLRYEGLFT
                     PPSTRGSLNLPGTIGGSEWGGAAYDPATATLYFKSNNSPEIDLLKKIAPETGTTTTTT
                     PWHTGRAVYMTYCTGCHKEDRSGDEPLYPSLIGLEKRRTRQQVIDKIRQGGGKMPPFA
                     ATIKGKEEALATYLLSANDRKLIHQESDLMEIRHNRSSLAETNPQKLITDTASAYLNV
                     LAYAPFRGSGNYPAIKPPWGELNAVNLNTGDYLWKIPVGNDSILAQKNGRPTGLTGSP
                     GPIVTAGGLVFIAGGKDRKLRAFDKDNGKLLWETTLPGMGSSTPCTYLCNGKQYIVLS
                     VAGDPQHPAGTLMAFALP"
     gene            2012..2836
                     /locus_tag="VKQ52_22560"
     CDS             2012..2836
                     /locus_tag="VKQ52_22560"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLZ90060.1"
                     /translation="MKNHLASYSLALSILVFSQCKSGLVTMSNNCYDLINQGQAQNKS
                     GNYSAALDNFNKVLQKCDAYDAKEKGYAGKAAALNGLQQYSDALDAANKGLAIDKTSL
                     DNLFEKASAETGLGNYTGARADLNTISDLTSKNRNTAQRATLFSKMAEVDTRQQQYDQ
                     ALQDMQQAIAIDNTNLRFYMQTGDINVAAGNYPAAIGNYNDAIAKGKNDAEAWKAKTT
                     TIIKMYQQKYKTQDAGTLSRKISSADRQTLCTSIEASKQAGLQDMGIDLVQQSICK"
     gene            2871..3533
                     /locus_tag="VKQ52_22565"
     CDS             2871..3533
                     /locus_tag="VKQ52_22565"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLZ90061.1"
                     /translation="MKHTLVFVPALIAVLLTDCKSMNNTQKATATGMVVGGAAGALIG
                     HGKNSVVDALIGTVVGGGAGYLIGRHMDKQAEEIKQAVPDAQVTRVGEGINMTFNSGL
                     LFKINSSLLSEEARTNLEKVAGIFVKYPETNILIEGHTDNTGTPEYNMELSKKRAYAV
                     SDYLASKGVAAGRMDIKWYGETQPKYPNDNDANRALNRRVEVGISANDQMKQKAQQGT
                     LN"
     gene            3675..3962
                     /locus_tag="VKQ52_22570"
     CDS             3675..3962
                     /locus_tag="VKQ52_22570"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLZ90062.1"
                     /translation="MNMYVSNLGFQVTNEELASLFEKFGEVTSAKVITDRETGRSRGF
                     AFVEMEDKAAELAMKGLDGTELDGRAISVTKAKPKTENKNSSFSRDRGNRW"
     gene            complement(4027..>4234)
                     /locus_tag="VKQ52_22575"
     CDS             complement(4027..>4234)
                     /locus_tag="VKQ52_22575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLZ90063.1"
                     /translation="FADFFEKEKPRWPSEKRDYLRNHHQSFEQFSSASISWEKLKVNG
                     LPDDILQEVRAAYDASQRGEEYGG"
BASE COUNT         1135 a         1347 c          990 g          762 t
ORIGIN      
        1 cgtatacgcc acatccgcaa gacaccgcgt atacgctatc tccgccatga caggccaact
       61 gcgctggtcc ttcgacccct tcgacggcgc cgaaggcggc ggcgtaagcc gcggcgtcac
      121 cttctggcag gatggcgccg atcgccgcat actcttcacc ggtggcgaca atcttttcgc
      181 cctcgacgcc aacaccggca aaccgatccc atccttcggg aacaacggca gagtgagcat
      241 gaacataggc atccgtgacg accccaatac catttctata aaacccacct ctcccggcat
      301 cgtctatcac gatctgctca tcatcggcaa cgaagtatcg gaactgtatg gcgcacaacc
      361 cggctacgtc cgcgcctatg ctattcgcac cgggaagctg gtatggacct tccacaccct
      421 cccccacccc ggagaacccg gctatgagac ctggcccccc gacgcctgga aatatgccgg
      481 cggcgcaaac gactgggccg gcatgagcct cgacgaaaga accggcaccg tcttcctcgc
      541 cctgggctcc ccctcctatg atttttatgg cgccgaccgc aaaggacaaa acctctacgg
      601 caattgcatc gtcgccctcg atgccgcgac aggaaaacat cgctggcatt ttcaaaccat
      661 acaccacgat ctctgggact atgacctccc cgccccgccc aacctggtca ctgtcacaaa
      721 aaatggtaaa cccatcgatg ccgtcgcgca gacctccaag gtcgggttcc tctatatcct
      781 cgaccgcgaa acgggaaccc cgctctatcc catcgaagaa cacagcgttc ccgcctccga
      841 cgtacccggc gaagaagcct ggcctacaca acccttcccg caaaagccgg cgccctatgc
      901 ccgccaatcc atgacaaagg acgatctcgc cgacttctcg ccggcagcac atgattccct
      961 ggtaaacagc ttccgctccc tgcgctatga aggcctgttc acgccccctt ccacccgggg
     1021 cagcctcaat ctcccgggaa ccatcggcgg gtccgaatgg ggcggcgcag cctatgaccc
     1081 cgccacggca accctctatt tcaaatccaa caactccccc gagatcgatc tgctgaagaa
     1141 gatcgcaccc gaaaccggta caacaacaac tacaacaccc tggcatacgg gccgcgcagt
     1201 ttatatgaca tactgcaccg gctgccacaa ggaagacaga agcggagacg aaccactcta
     1261 tccttcactc atcgggctgg aaaaaagacg gacccggcaa caggtgatcg acaagatcag
     1321 gcagggtgga ggaaagatgc cacccttcgc cgcaacgata aaaggaaagg aagaagccct
     1381 cgccacctac ctgctctccg ccaacgaccg gaaactgatc caccaggaat ccgatctgat
     1441 ggagatccgg cacaaccggt catccctggc ggagacaaat ccccaaaaac tgatcaccga
     1501 cacggcctcc gcatacctca acgtattggc ctatgcgccg ttcaggggct ccggcaacta
     1561 tcccgccatc aagccgccct ggggcgaact gaatgccgtc aacctcaaca cgggtgacta
     1621 tctgtggaag atccccgtcg gtaacgacag catcctcgct cagaagaacg gccggcccac
     1681 gggactcacc ggctcaccgg ggcccatcgt caccgccggc ggactcgtat tcatcgcagg
     1741 cgggaaagac aggaaactaa gggccttcga caaggacaac ggtaaactat tatgggaaac
     1801 aacattgccg ggcatgggct cctctacacc ctgcacctac ctctgcaacg gcaaacaata
     1861 tatcgtcctc tccgtcgccg gcgatccgca gcatcccgcc ggtaccctca tggccttcgc
     1921 actgccataa gcaggtccac acctcttccc gtttttttca ttttcgactt tgacaatttt
     1981 acccatatat ttgcagcgct aatcgaataa catgaaaaat cacctggctt cctacagtct
     2041 ggccctgtct attttagtct tctctcaatg caaaagcggg ctggttacta tgtccaacaa
     2101 ctgctatgac ctcatcaacc agggtcaggc acaaaacaag tccggcaact actccgccgc
     2161 actcgacaac ttcaacaaag tccttcaaaa atgtgacgcc tacgatgcaa aggagaaagg
     2221 ctatgccgga aaagcagcag ccctcaacgg actgcaacaa tactccgacg cgctcgacgc
     2281 cgcaaacaaa ggcctcgcca tcgacaagac aagcctcgac aacctctttg aaaaggcaag
     2341 cgccgaaaca ggcctgggca actacaccgg agctcgcgcc gatctgaaca cgatctccga
     2401 tctcaccagc aagaaccgca acaccgcgca acgcgccacc ctcttctcga aaatggcgga
     2461 ggtcgacacc cgccagcagc agtacgacca ggccctgcag gatatgcaac aggccatcgc
     2521 catagacaat accaacctcc gcttctatat gcagacgggc gacatcaacg tcgcggccgg
     2581 gaactacccc gcagccatcg gcaactacaa cgatgccatc gcaaaaggca agaacgacgc
     2641 cgaagcctgg aaggctaaaa caacgaccat cataaagatg taccagcaga aatacaaaac
     2701 ccaggatgcc ggaacgctgt ccaggaagat cagcagcgcc gaccggcaaa ccctgtgtac
     2761 cagcatcgaa gcatcgaaac aggccggcct ccaggacatg ggtatcgatc tggtccagca
     2821 atccatctgt aaatgagcaa atgatcctga cacttcaaat tagctaacac atgaaacaca
     2881 ccctcgtatt tgtacctgca ttgatcgcag tgctgctgac agactgcaaa agcatgaata
     2941 acacccaaaa agcaaccgcc accggtatgg tagtgggtgg cgccgccgga gccctcatcg
     3001 gtcatggtaa gaattccgtc gtggacgccc tcatcggcac cgtcgtcggc ggcggcgctg
     3061 gctacctcat cggccgccat atggacaaac aggccgaaga gatcaaacag gcagtccccg
     3121 atgcccaggt cacaagagtg ggcgaaggga taaacatgac cttcaattcc ggtctgctgt
     3181 tcaagatcaa ctccagcctg ctcagcgaag aagcccgcac caatctggaa aaagtagccg
     3241 gcatcttcgt aaaatatccc gagaccaata ttctcatcga aggacatacc gacaacaccg
     3301 gtacccccga gtacaatatg gaactctcca aaaaaagagc ctatgccgta agcgactatc
     3361 tcgcaagtaa gggtgttgcc gccggccgga tggacatcaa atggtatggc gagacccagc
     3421 caaaataccc caacgacaac gacgccaacc gcgccctcaa ccgccgcgtc gaagtcggta
     3481 tcagcgccaa cgaccagatg aaacaaaaag cccagcaggg aaccctcaac taaaagtgat
     3541 ggccggggcg cccaacgccc ccgctcaccc cattaatatt attattaggc cacccaggta
     3601 taaaagctta tctttatcgt ttgctctgct ttttattgca tggcaaagta ggttaacact
     3661 tttaatttga atttatgaac atgtacgtct cgaacctggg cttccaggtg accaatgaag
     3721 aattagcgtc cttatttgaa aagtttgggg aagtcacttc cgcaaaagtg atcaccgacc
     3781 gggaaaccgg aagatccagg ggatttgcct ttgtggaaat ggaggataag gctgccgaat
     3841 tggcaatgaa gggattggat ggaaccgagc tcgacggaag ggccatatcg gtcaccaagg
     3901 caaaacccaa gaccgagaat aagaacagct ctttttcacg cgaccgggga aaccgttggt
     3961 aaaaccctcg ggtgacaacc aaaagcctcc tgatccctgg tcaggaggct tttagagtta
     4021 tcggaactat ccgccatact cctcaccccg ttgggatgca tcgtaagcag cgcgaacctc
     4081 ttgcaagata tcgtcaggca atccatttac ttttaatttc tcccaggaga tagaagcaga
     4141 ggagaattgt tcaaaactct ggtgatgatt tcgcaggtag tcgcgcttct cgcttggcca
     4201 acgcggtttt tctttttcga agaaatctgc gaat
//
